LOCUS       DAOQUC010000001        11857 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_6089_length_11857_cov_3.601762, whole genome shotgun sequence.
ACCESSION   DAOQUC010000001 DAOQUC010000000
VERSION     DAOQUC010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 11857)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11857)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11857
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6089_length_11857_cov_3.601762"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..657
                     /locus_tag="PKH07_00005"
     CDS             <1..657
                     /locus_tag="PKH07_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33365.1"
                     /translation="VYKSSNKGVSWGTVRYLGTAIYADSRCTDIAVAPSNGSIVYALG
                     IENRGPRIYRSSDGGSNWSDVTGNLRDFHSGTSHYGYAVHVAANAPDVVIVGTSLGAF
                     ISTNGGSSWSATMLSQPVKAFAADPRQEMLFAATLYQGVYVTIDGGYSWEKMNNGLSS
                     LNCIDIECDPMNRLLFVGTSGGGVFRVSTEMIPTPTMTPQNTPTPQTSGVAGGWRDYD
                     "
     gene            790..990
                     /locus_tag="PKH07_00010"
                     /pseudo
     CDS             790..990
                     /locus_tag="PKH07_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020249000.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
     gene            complement(1635..3881)
                     /locus_tag="PKH07_00015"
     CDS             complement(1635..3881)
                     /locus_tag="PKH07_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33366.1"
                     /translation="MFSHILLSLAAIVSLPEGSAPSPVICPHFPNALYAFVWRNWQLV
                     PVDRMASVVGASPEQILQIGHGMGLNGPPEITPEQQRRSCITVIRRNWHILPYEQLLD
                     LLGWTESEMAYTLREDDFLFHKLGNLKPKCAPIRYAEPDAETRLEQERIAKVVERFFP
                     GGVGLLNEPLFDFIQDLSAPLNPSAAFSSVRQSEPRFCYSYFALYGDPLLDPSLDPYP
                     DGYLARLAQLGVNGVWLQGVLYKLSEFPWDPSLSEGFETRLENLRQLVARTKQKGIGV
                     YLYLNEPRAMPESFHQKNPGLKGVSEGDYSTLCSSVPEVQAYLTHAVESICRAVPDLA
                     GFFTITASENLTNCWSHYQGAHCPRCSSRPAGDVIAEINQLIQAGIERAGSSTRLIAW
                     DWGWQDDWAIQAIEKLPQAVSLMSVSEWSTPIQRGGVNCSVGEYSISTIGPGPRATRH
                     WEAAHKSGLKTLAKVQVGNTWELSSVPYIPALENVAKHAANLRDVGVDGLMLGWTLGG
                     YPSPNLEVFSEIGRDPNISTDDALIRVAERRFGTAVAPAIVQAWRSFSTAFSEFPFHG
                     SLVYLAPLQMGPANPLWEQPTGYRATMVGFPYDDLDSWRASYPPEVYTGQFRKIADGF
                     DDALEVLHSSIKKDVAVITQAQRMNLERELVVAQAAAIHFRSVLNQAEFVVARKAYNE
                     SPSDETRQTLERCLISEIDLAQRLYALQASDSRLGFEASNHYFYVPMDLAEKVLNCQD
                     LLNRWLSR"
     gene            4117..5085
                     /locus_tag="PKH07_00020"
     CDS             4117..5085
                     /locus_tag="PKH07_00020"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HNT33367.1"
                     /translation="MSSPSVRDRNASENKVHKGEPSCGCCCCDATSSGTKTPRLVFRS
                     PGIGTWQPRPLDHIRWNLTRKGRILRKADAIVVSVPKCGRTWLKVFLKAYFSAVENRD
                     FSLLFHGSDRTMDVRFAHDLWEHLVRASLRQRLLGKHLVPASARCTSKILLLVRDPRD
                     VIVSLYFHLTKRSRIFKGSLSEMIRHRRFGIESVIWMLNTWMKEWHDYDNIHIFRYED
                     FQTDPRSTFQSVLAFLGFPTIDVEKFAGALKTASFDSMREMEATGKADHKCLRPADAQ
                     DPDSFKVRRGIVGGYKDYLDEGDLSYIEEAMKLLDPRLGYTVESRS"
     gene            5158..5592
                     /locus_tag="PKH07_00025"
     CDS             5158..5592
                     /locus_tag="PKH07_00025"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNT33368.1"
                     /translation="MKSRILIVDDDRNVIHMLVLFLVDLGYEVIPARDGLSAVTLAVK
                     YLPDLIILDVMMPKMSGYQTCEFIRRKEGLQNLPIVFLTAKGQPHDEEYGKKIGGSAY
                     LTKPINFPVLEETIATLIHEHPMDFPNRPAVDLTDPGIQWVG"
     gene            complement(5598..6821)
                     /locus_tag="PKH07_00030"
     CDS             complement(5598..6821)
                     /locus_tag="PKH07_00030"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HNT33369.1"
                     /translation="MKRNSTTGWVLLITLVLVSQTSASWAQQTGALQPPALPPAAGTL
                     ERTVQSASPEAAGLTVLGTPEIFCPEPTKSFGVLYVSEEWKHSFVVTNKGTADLIINN
                     VTKDCGCTSASADAKQLTPGQSTNINVAFNSGQHPTKTKKKVTVLSNDPVNPKLALEF
                     VGEVTSAFRFSPPTFPPFGAMTKGASSERSIDIFVAVKEPVELTGVEGPSPLVQKSLI
                     KIADEPERHYRLTVRLEIPEDFPERFVRDDIIVKTTFEREKELRFAIYAGIEPEVSLT
                     PSFMNFHSVQKSQHPSRTVYIQSRRNKDFQITGIEHSLAGMEIEAASESLGPEGGTRY
                     KITAKMTDEFSQEGFFRDQVLLRTNLKSQPEIQLFVYGSLLAQQGVGMQPPLPQAVAP
                     PPVQNTVEQPEQHAE"
     gene            complement(6978..7799)
                     /locus_tag="PKH07_00035"
     CDS             complement(6978..7799)
                     /locus_tag="PKH07_00035"
                     /inference="COORDINATES: protein motif:HMM:NF024860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HNT33370.1"
                     /translation="MGKARIEAMESTTLITPRSEGPDFVGIGVQKCGTTWLGDILSQH
                     PDVLVEKKEISFYTHHFHKGYNWYHNWFKEKNGRIAGEITVSYLFLPRPDSFHKEFYP
                     KRNPKRTLLFWRKQPSARDQLLEHYPGLRVFAIFRNPADRAWSHYWFWRNRRERMGKR
                     IVPFEDFFAADARWVQTHGLYADHLAYWREAFPDMGVYLFDDLKNDPDTLIKDLYRFV
                     GVRDDFTPVIGEKVNAGRYEKMPKEVRTQLIAHYRDQIERFSVMIGRDLTPWLQR"
     gene            8174..9217
                     /locus_tag="PKH07_00040"
     CDS             8174..9217
                     /locus_tag="PKH07_00040"
                     /inference="COORDINATES: protein motif:HMM:NF021285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HNT33371.1"
                     /translation="MRSEVFAKFFAESLDIAVIWSLTRFFLSRSHHHTEPSELEQLLH
                     LYQSADPDHVFSDPCPTDEFAIQSAHHLFGHSTEEFVFPSAVQTPIERNNTVHARRIR
                     SGSLEGKPVLILVPGWIIGHLDPLLWYFGRPLLRMGISSVVIEPPYQMRRTPSGSWTG
                     EYTISGNMVRTFDSIRQAVLDVRRLIAILRQQGASRIGVMGMSMGGWITSLVSVVETD
                     LALSAIVVPPVDLTHILKTSPLLRTIRNDIIQDGLLDQDVSRVSFLLSPLSYPLRVPL
                     DRVRLFQARHDRALPPKGVEDLWNHWGRPLLRKYSAGHISIVLSPGFLRDFRRDCRQL
                     LLDGVPGDSRRTS"
     gene            9266..10621
                     /locus_tag="PKH07_00045"
     CDS             9266..10621
                     /locus_tag="PKH07_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012097168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNT33372.1"
                     /translation="MHSVLFVDDEERLGRILTKAVETMSGWTAESVTSAEDALKRLEE
                     FPFDVIVSDIRMPEMNGIELLDRVKKIRPQTEVVMMTAYGTVENAVSAMKLGAADFLV
                     KPVKTHDLKRLLNRLFALASEGEPAAVTMEEEQSEFSFDSVIAKSASMRRVLEMARKV
                     AAKNATVLLRGESGTGKDLLAQVIHYNSPRASHPFVKAVCAAIPEGLLESDLFGHIKG
                     AFTGATEAKPGRFQLADTGTIFLDEIGDMTLNTQVKLLRVLQDRQFEPVGSTKTIGVD
                     VRVIAATNRDLEEAIEESQFREDLYYRLNVVTLVLPPLRERPEDIPPLIDRLLSDSAR
                     REGRDLCRLTEGARSLLLRHPWPGNVRELQNALEHAEIMTGNDEITENELPLYLQARN
                     PAPSPEAPSALLDGMTLEQMERRLIQKALEDSGGNATKAADMLGITRRTLGYRREKYQ
                     L"
     gene            complement(10612..11733)
                     /locus_tag="PKH07_00050"
     CDS             complement(10612..11733)
                     /locus_tag="PKH07_00050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33373.1"
                     /translation="MDRLKNPLTFTTEPSLKRIGFYLVFAFSVSLAFLILWSETTLGF
                     IPVLLFLIASLVLWIVILRWQIRADEKGIAVRKLLSWDWWRWEDFENGRIYRSVEPDG
                     FIRFTPDLRIQHLSFAGFRYEDRELLMSLCLKLWKPPAGTDIPKEISVWFPRFLPKFL
                     QTHLCLSPEGLRLTHRGRNRDYLWNDVQAVMIGRSTHNHQNFRYLLVALPDRVIRLSS
                     GSGMGSLWSGSVPEWIVEFLSRHVPEEKRESFVFHEPPINRQELEGYLHYRAQTLAST
                     QRDFPYLMIFFVLPVLSFPLLIGLSESMRHDTKIPIAGIVCWLSLLALAALILFLFVP
                     WQHRKLLARERARLENLLLWINPEGVKEASSERPETLQS"
BASE COUNT         2758 a         3132 c         3204 g         2763 t
ORIGIN      
        1 gtctacaaga gttcgaataa gggtgtctct tggggcacag tacggtatct gggtacagca
       61 atctatgctg atagcagatg cacggatatc gctgtcgctc cttcgaatgg atccattgtc
      121 tatgccttgg gcatagagaa tagaggtccg agaatctacc gttcgtcgga tggtggatcc
      181 aactggagtg acgtgacagg aaacctccga gacttccact ctggaacgag ccactacggc
      241 tatgcggttc acgttgcagc aaacgcacct gacgttgtca tcgtcggaac cagcctcggg
      301 gcgtttatct caaccaatgg tggatcttct tggagcgcga cgatgctcag ccagcccgtc
      361 aaggcctttg cagccgaccc gaggcaggaa atgctgtttg cggccacact ttatcaggga
      421 gtctatgtca ccattgacgg cggctattcc tgggagaaga tgaacaacgg gctaagttct
      481 ctgaactgca tagacattga atgcgatccg atgaatcgtc tgctgttcgt gggaacttcg
      541 ggagggggag tcttccgagt ttcgacagag atgataccga caccgaccat gacaccacag
      601 aacaccccga caccacagac cagtggagtt gcgggtggct ggagagacta tgactaaagt
      661 agtgccgtgc ttctttccgc agaagactgt gtaggaagga agcctccagg acgagggctt
      721 tccgcctgaa gcctttccgt caaagcacaa taggcgtttg ggaatgtaca gaattggagg
      781 atgaaagata tgaagaacga gagaactttg ttgaggagac tgctgcctat tcagtctgcg
      841 tgtcggcgac agtaccagtc aggcttcacg ctcatcgaat tgctcatcgt ggttgcgatc
      901 atcggcatac ttgctgccat agcaattccc aatttcctcg ccgcccagac tcgagccaag
      961 tgtgccaggg cgaaggcgga cttgcaggca ttctccggcg caattgaact cgtcttcaca
     1021 gatggcggag actatccccc caatgatgga cagacgggag gcgcacctct tcagatcaca
     1081 acacccatag cgtatctgca acaaggggcg ctcatagatc cgttctcgac agcaaaagac
     1141 gatcccgtgc tcggatcccg accaaggttc tacaaataca acaaggtctt gagtcagacg
     1201 caactgagcg atttagtctc ggcaggcgct cctcttccgt ctccactggc aattgatgca
     1261 ccgggttaca acgtgggtgc tctcatcaag tacggaaagt ggcgtatcgc ttcccttggt
     1321 cccgacggac tctatgcgga tcccgcttcc acagatctga tcctgtttgg agccgacacg
     1381 ccctacgatc cgaccaatgg agtaacgagc aagggaaaca tcgtgagaac ccagaagagc
     1441 agcacaggca agtatcaagg ttgcgtgact tgttggtaga agggacttgt cctatggcct
     1501 gaggactctt gtcgctgatt ggaacgactt cttcatggag acgtcggaac gtccatggtc
     1561 ggaaagcatt tcctgagcgc ggccgaaagg acttcctcat ccgctacaat gagggcaaac
     1621 acgcaggtgc cttcctacct gctgagccat cggttcagga gatcctggca gttcaacacc
     1681 ttctcggcga gatccattgg tacatagaag taatggtttg acgcctcaaa cccgaggcgg
     1741 gagtcgcttg cctgaagcgc atacaggcgt tgcgccagat cgatctcact gatcaaacag
     1801 cgttccagag tctgccgcgt ttcatcagag gggctctcgt tatacgcctt ccgtgccacc
     1861 acgaactcgg cttgattgag aacacttcga aagtggattg ctgcggcttg cgctacgacc
     1921 aattcccgtt ccaggttcat tcgctgtgct tgagtgatga ccgcgacgtc cttcttgatc
     1981 gaagagtgca ggacttcgag agcatcgtca aacccgtctg cgatctttct gaactgtcca
     2041 gtgtaaacct ccggtggata ggaggctctc cagctatcca gatcgtcata gggaaagccg
     2101 accatcgttg cgcgatatcc cgtcggctgc tcccaaaggg gatttgctgg ccccatttga
     2161 aggggtgcga gatagaccaa tgaaccgtga aaaggaaact cgctgaaggc cgtgctgaaa
     2221 ctccgccatg cctgcacaat ggcaggcgca acggccgtgc cgaatcgtcg ttccgctacc
     2281 cgaatcagag cgtcatcggt agagatgttc gggtcgcgcc cgatttcgga aaacacttcc
     2341 agattcggcg aaggataccc acccagcgtc cagcccagca tcagaccgtc cactcccaca
     2401 tcgcgaagat tcgcagcgtg ctttgccaca ttctccaatg ctggaatata gggcaccgag
     2461 gacagttccc aagtgttgcc cacttgaacc ttggcaaggg tcttcagccc gctcttgtgc
     2521 gcggcttccc agtgccgcgt tgcacgaggc cctggcccga tggtcgaaat ggagtattcc
     2581 cccacagagc aattcacgcc gccgcgctgg attggggtac tccattcgct cacgctcatc
     2641 agggaaacgg cttgaggcag cttctcgatg gcttgaatcg cccagtcgtc ctgccaaccc
     2701 cagtcccagg caatcaagcg tgtgctggaa ccagctcgtt caatacccgc ctgaatgagt
     2761 tggtttattt ccgcgatcac atcccccgcg ggacgacttg agcaacgtgg acagtgtgcc
     2821 ccctgatagt gtgaccagca gtttgtcaga ttctccgagg cggtgatagt aaaaaagcct
     2881 gccagatcag gaactgcacg acagatgctc tcgaccgcat gggtcagata cgcctgaact
     2941 tctggaacgc tggaacacaa cgtggaatag tcaccctcgc tgactccctt cagtccgggg
     3001 ttcttctgat gaaaggattc gggcatcgcc cggggttcat tgaggtacag gtagacgccg
     3061 attcccttct gctttgtcct cgccaccagt tgtcgaaggt tctctagacg agtttcaaag
     3121 ccttcactca aacttggatc ccatggaaac tcagaaagct tgtacaacac tccctggagc
     3181 cacacaccat tcacacccaa ctgagccagt cgagccaggt atccatcggg atagggatcg
     3241 agagacggat ccagcagcgg atccccgtac aacgcgaagt aggaataaca gaatctcggc
     3301 tcagattgcc gcacagatga gaacgccgcg gaaggattca gcggcgctga cagatcttga
     3361 atgaaatcga acagtggctc attcagaaga ccgacgccac cagggaagaa ccgctcgacg
     3421 accttggcaa tgcgttcctg ttcgaggcgt gtctcagcat ccggctcggc gtagcggatt
     3481 ggagcgcatt tgggtttcag attgccgagt ttgtgaaaga ggaagtcgtc ttctcgaagc
     3541 gtgtaggcca tttccgattc cgtccatccg agaagatcga gcagctgctc atagggaagg
     3601 atgtgccaat tgcgtcggat cactgtgatg caagagcgtc tctgctgctc cggtgtgatc
     3661 tcaggcgggc cgttcagccc catgccatgg ccaatctgga gaatctgttc cggcgatgct
     3721 cccacgacgg atgccatccg atcaacggga accagctgcc agtttcgcca cacaaaggcg
     3781 tagagggcgt tggggaaatg tggacagatt acgggggaag gtgccgatcc ttcgggaaga
     3841 gagacgatcg cagccagaga gagcagaatg tgggagaaca tgagagcctc ctttggtaat
     3901 gaaatcctgg caccaaccta atacgcgacg tgaggtgatg caaccacgtc catcttcttg
     3961 taatgcttcg gccatacctg cgcggaacaa ccaaaccctg tgtatccgaa ccgatacaaa
     4021 cactatctat cttgtcttgc gaaaatcatg catctcactt acagtcccaa ctatcgagag
     4081 gcagccggcc tgcctcgtga atagggagga gtggagatga gttctccttc tgttagggac
     4141 aggaatgctt ccgagaacaa agttcataag ggagagcctt cctgcggctg ttgttgctgc
     4201 gatgcaacgt cgtctggaac aaagacacca cgtctggtgt ttcgtagtcc gggcatcggg
     4261 acgtggcagc ctcgaccact ggatcatatt cgctggaacc tgactcgaaa ggggcgaatc
     4321 cttcgcaagg ccgacgccat tgtggtgtct gttcccaagt gtggaaggac gtggctcaag
     4381 gtattcctga aagcttattt ctccgctgtt gagaatcgtg atttctcgct cctgttccat
     4441 ggctctgatc gaacgatgga cgttcgcttt gctcacgatc tctgggagca tcttgtgcgc
     4501 gccagtcttc gtcaaaggtt gctaggcaaa catctcgtgc ccgcctcggc acgatgcacc
     4561 agcaaaattc tgctcttggt tcgggatcct cgggacgtca ttgtctccct gtacttccat
     4621 ctaaccaaga ggagccgcat cttcaaggga tctttgtcgg agatgatccg ccaccgcaga
     4681 ttcgggattg aatctgtgat ctggatgttg aacacctgga tgaaggaatg gcacgactac
     4741 gacaatatcc acattttccg ttacgaggat tttcagacag atcctcgaag cacattccag
     4801 tcggttcttg ccttcttggg ctttcccacc attgatgtgg agaaatttgc tggggcgctc
     4861 aagaccgcgt ccttcgactc gatgcgtgaa atggaagcca cgggaaaggc agatcacaaa
     4921 tgtcttcgac ccgcggacgc tcaggatccc gactccttta aggttcgtcg cggtattgtc
     4981 gggggctata aggactatct tgacgagggg gatctttcct atatcgaaga agcgatgaaa
     5041 cttctggatc cccgcttggg atacactgtc gaaagcagat cgtgaaggat ctggaacaga
     5101 taggtaaact ggctctggtt ggcgtgggca gagtttttct ttcaggaggt gccagggatg
     5161 aagagtcgta ttctcattgt ggacgatgac aggaatgtca tccacatgct ggtgctgttt
     5221 ctcgtggatc tcggctatga ggtgattcct gccagggacg gtctcagtgc ggtgacactg
     5281 gctgtgaagt acttgcccga tctgatcatc ctggatgtca tgatgcccaa gatgagtgga
     5341 taccaaacat gtgaattcat ccgacgcaag gaagggctgc agaacctgcc cattgtgttc
     5401 ctcactgcga aggggcaacc acacgacgag gagtatggga agaagatcgg aggctcagcc
     5461 tatctgacca aaccgatcaa cttccccgtt ctagaggaaa ccatcgcgac actcattcac
     5521 gaacacccga tggactttcc caatcggccg gctgttgatc tgacagatcc tggcatccag
     5581 tgggtcggct gagtacccta ctctgcatgc tgttcgggtt gctcaacagt gttttgtaca
     5641 gggggaggcg caaccgcttg cggtaacgga ggctgcatgc ccactccctg ctgcgccaac
     5701 aggcttccgt agacaaagag ttgaatctcc ggttgcgact tgaggttggt tcgaagcaga
     5761 acctgatcgc ggaagaatcc ttcctggctg aattcatcgg tcatcttggc ggtgatctta
     5821 tatcgagtac caccttcggg accaagagac tccgaagccg cttcaatctc catgcctgcg
     5881 agactgtgct cgatgccagt gatctgaaaa tccttgtttc ggcgactctg aatgtacacg
     5941 gttcgagagg ggtgctggct cttctgaacc gagtggaaat tcatgaaact tggggtgagg
     6001 gaaacttcag gctcaatgcc tgcgtagatg gcaaaacgaa gttccttctc cctctcaaaa
     6061 gtggtcttga cgattatgtc atcgcgaaca aagcgttcag gaaaatcctc tggaatctct
     6121 agtctgacag tcaaacggta gtggcgttcg ggctcgtcgg ctatcttgat gagcgatttc
     6181 tgcaccagag gtgaggggcc ttccacgcca gtcagttcga cgggctcctt taccgcgacg
     6241 aaaatgtcta tggatctctc actggaggca cctttcgtca tagcgccaaa cggcggaaac
     6301 gtcggaggag agaatcggaa cgcagacgtg acttcaccaa caaactccag agccagtttg
     6361 gggttaaccg gatcgttgga gaggacggta accttcttct tcgtcttggt cgggtgttga
     6421 cctgagttga aagccacatt gatatttgtg gactgtccgg gcgtgagctg tttggcgtca
     6481 gccgacgcgg acgtgcagcc acagtccttg gtcacgttgt tgataatcag atcggcggtt
     6541 cccttgttgg tgacaacaaa cgagtgtttc cattcctcgc tgacatagag aacgccgaag
     6601 ctcttggtcg gctctgggca gaatatctcg ggcgttccta gaacagtcag tcctgccgct
     6661 tcgggtgatg ccgattgaac ggttctttcc agggtgcctg ccgccggggg aagagcaggc
     6721 ggctgaaggg ctccggtttg ttgcgcccag gaagcggacg tctgactgac aagaaccaaa
     6781 gttatcaaaa gcacccaacc tgttgtggaa ttcctcttca ctgctgaatc tccttcatgt
     6841 tgttcgtcaa cgtcaggaat tatcatcccg cgagactcag gctgtcaacc tgcattgttg
     6901 cgaatgccct atccgcagct cagagagact tgttctgctg gttttccgga cgggtagtgc
     6961 cagtctcgca cggcgagtca tctttggagc cagggagtga gatcgcggcc tatcattacc
     7021 gaaaagcgtt cgatctgatc tctatagtgc gcaatcaact gagtgcgcac ctctttgggc
     7081 attttctcat agcgacctgc gttgactttc tctccgatta ccggagtgaa atcatcacga
     7141 acaccgacaa aacggtagag atccttgatc aacgtgtcag gatcattctt cagatcgtca
     7201 aaaagatata cacccatgtc aggaaaagcc tcacgccagt acgccagatg gtctgcatac
     7261 aggccatggg tttgcaccca acgtgcatcc gccgcgaaga aatcctcgaa tgggacgatt
     7321 ctcttgccca tgcgctccct gcggtttcgc cagaaccagt aatgcgacca ggcacggtct
     7381 gctggatttc gaaagatggc aaacacgcga agtccaggat agtgctccag aagttgatcg
     7441 cgggctgaag gttgctttcg ccagaaaagt agtgttctct tgggatttcg cttggggtag
     7501 aactccttgt gaaaggaatc aggccttggg agaaagagat aactgacagt gatctcgcct
     7561 gcgattcggc cgttcttctc tttgaaccaa ttgtgatacc agttgtagcc cttatggaaa
     7621 tggtgtgtgt agaaactgat ttctttcttc tcaaccaaga cgtccggatg ctggctcaga
     7681 atgtcaccga gccaggtcgt tccgcatttc tgaactccga taccgacaaa gtcggggcct
     7741 tcacttctgg gagtgattag agttgtactt tccatcgcct cgattcgcgc ctttcccaag
     7801 atctcctttg tatatcactc atcgttcgca ggcgctggcc tgctcaggag cacgacaggg
     7861 agagtctaag gcgttcagat gctttgtcaa gttcaggtgg atcacagaaa ggtcgaatcc
     7921 acccgatgag gcaagaagga aagccctttt cgtggtcctt catctgtggt tgtcgagtga
     7981 ctaatccgcg gtttgacaga ccctcgtgat tgctgttgaa tccttctgca aaggcatttc
     8041 gcagactgta cggcgctctg cggagtccgt tgcatcgctt gcccgcctat ggatgaggtt
     8101 cgaatagggt gcgcggtgcc agttgatatt ccgatcaagc gtccgggatt tgagtgcctt
     8161 cgttctcttg gacatgaggt cagaagtgtt cgccaagttc ttcgctgaaa gcctggacat
     8221 cgcagtcatc tggtcgctga ctcgcttctt tctgagtcgc tctcaccacc atacggagcc
     8281 gagtgagctc gagcaactcc tgcacctcta tcagtctgcc gatccggatc atgttttctc
     8341 tgatccctgt ccaacagatg aatttgcgat tcagtctgca caccacctgt ttggccacag
     8401 cactgaggaa tttgtctttc cttcagcagt gcaaacacca attgagagaa acaacaccgt
     8461 ccacgcgcga aggattaggt ctggttccct cgaaggaaag cctgtgctca ttcttgttcc
     8521 aggttggatt atcggccatt tggatccgct gttgtggtat ttcggtcgtc cccttcttcg
     8581 gatgggcatt tcttccgtcg tgatcgagcc accctatcaa atgcgaagga ctccttctgg
     8641 ctcctggaca ggggaataca ccatttcagg aaacatggtc agaactttcg acagcatacg
     8701 acaggccgtt ctggacgtgc gacgcctcat cgcgatcctg agacagcagg gagcatcccg
     8761 catcggggtc atgggcatga gtatgggtgg ctggattacc tccctggtca gtgttgttga
     8821 aaccgatttg gctttgtctg ccattgtcgt tccgcccgtt gatttaacgc acattctcaa
     8881 gacctctcca ctgcttcgga cgattcggaa cgatatcatc caggatggtc tcttggatca
     8941 ggatgtgtct cgagtctcgt tccttctgtc acctttgtcc tatcctctgc gagtgcctct
     9001 ggatcgggtc agactttttc aggctcgcca tgatcgagcc ttgcccccaa aaggagtcga
     9061 agatctttgg aatcattggg gaaggcctct tctcagaaag tacagtgcgg gacatatcag
     9121 catcgtcctt tcacccggat tcctcaggga ttttcgcagg gactgtcggc agttgctttt
     9181 ggatggtgtt cccggagatt cccgtcgaac atcgtgaacg aatgcgatac gatgatcccg
     9241 atgtggagta tcaggagtga ccgttatgca cagtgttctc tttgtggacg atgaggaaag
     9301 gctcggacgc atcctgacga aggctgtgga aaccatgtcg ggctggactg ctgagtcggt
     9361 cacatcggcg gaagacgctc tcaaacgcct tgaagagttt ccctttgatg tgatcgtatc
     9421 ggatattcgg atgcctgaga tgaacggcat cgaactgctt gatcgcgtca agaagatccg
     9481 tcctcaaact gaagtcgtca tgatgacagc ctacggaact gttgaaaatg cggtttcagc
     9541 gatgaaactg ggcgccgcag acttccttgt aaaacccgtc aagacgcacg atctgaagcg
     9601 ccttctgaac cggctgttcg ctcttgcctc ggaaggagag cctgctgccg tcactatgga
     9661 agaggagcaa tcggagtttt cgttcgactc tgtgatcgcg aaatcggctt cgatgcgccg
     9721 agtgttggag atggcacgca aggtcgctgc caagaacgcg acagtgttgc ttcgtggaga
     9781 gtccggaacc ggcaaggatt tgttggcgca ggtgattcat tacaattcac cgcgcgcttc
     9841 acatcccttt gtgaaggccg tctgtgcggc cattccggaa ggacttcttg agagcgatct
     9901 tttcggtcac atcaagggag cgttcaccgg agcgacggaa gccaagcctg gccgtttcca
     9961 gttggcggac acaggcacca tattccttga cgagattggc gacatgactc tcaacacgca
    10021 ggtcaaactc cttcgagtgc ttcaggatcg acagtttgaa ccggttggaa gcaccaaaac
    10081 aatcggtgta gatgttcggg tcattgcggc aaccaatcgc gatcttgagg aagccatcga
    10141 ggaatcgcaa ttccgagaag acctctatta ccgtctcaac gttgtgacac ttgttctgcc
    10201 accacttcgc gagagaccgg aagacattcc gccgcttatt gaccggcttc tgagcgactc
    10261 agcgcgccgg gaaggcaggg atctctgtcg tctcaccgaa ggtgcccgct ctctcctgct
    10321 cagacatcca tggcctggaa acgttcggga actccagaat gctttggagc atgcggagat
    10381 tatgacaggc aacgatgaga tcaccgaaaa tgaactgccc ttgtatcttc aagcaaggaa
    10441 tccagccccc tcgcccgaag caccatccgc attgctggac gggatgacgc tggaacaaat
    10501 ggaacgcagg ctgatccaga aagcgctcga agattctgga ggcaacgcca ccaaggcggc
    10561 ggacatgctt ggtattactc gacgcacgct gggatatcgt cgtgagaagt atcagctctg
    10621 aagtgtttct ggtctttcag acgatgcttc cttgactcct tccgggttta tccacagcag
    10681 aagattctcc aaccgtgctc gctctcgtgc gaggagcttt ctatgctgcc aaggcacgaa
    10741 caggaacagg atcaatgcag cgagcgccag gagtgacaac caacagacaa tccctgcgat
    10801 tgggattttc gtatcgtgtc gcatagactc agagagacct atcaggagag gaaacgacag
    10861 aactggcaga acaaaaaaga tcatcagata agggaaatct ctctgcgtgg aagcgagagt
    10921 ctgtgctctg tagtgcagat aaccttccaa ctcctgacgg tttatgggag gctcatgaaa
    10981 tacaaaagac tctcttttct cctcaggaac atgtctgctc aggaattcga cgatccattc
    11041 gggaacggaa ccactccaga gcgaacccat tccggaaccc gacgatagcc ggatgacccg
    11101 atccggcagc gcgaccaaaa gatagcggaa gttttgatga ttatgggtgc tgcgaccgat
    11161 catcacagcc tgaacatcgt tccacaaata gtctcgattc ctcccacgat gggtcaagcg
    11221 aagaccttcc ggggacaggc acagatgcgt ctgaaggaat ttcggcaaga accgggggaa
    11281 ccaaacagaa atctccttgg ggatatctgt tcccgcgggt ggtttccaga gtttgagaca
    11341 gagtgacatc agcaattccc tgtcctcata gcggaagccg gcaaaggaga gatgctggat
    11401 gcgcaaatca ggagtgaatc ggatgaaccc atcaggctcc acagagcggt agatgcgtcc
    11461 attttcaaag tcttcccacc gccaccaatc ccatgacagc agttttcgca cagcgatacc
    11521 tttttcatcc gccctgatct gccagcgcag gatgacgatc cacaacacaa gcgatgcgat
    11581 gagaaacaga agcactggga tgaatcccag agtggtctcc gaccaaagaa tcaggaatgc
    11641 caggctgacg gagaaggcaa aaaccagata gaaccctatt cttttcaggg acggctcggt
    11701 cgtaaacgtc agcggattct tcagtctgtc catttcctaa gtagtttccc acactggatg
    11761 tcacttttca actccaactc ctttttcgtg tggtaaagca cggatacaat ctgaagatag
    11821 tcagttgatc acaacccatc ttccccaatt tgacaag
//
LOCUS       DAOQUC010000002        10707 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_6926_length_10707_cov_2.876361, whole genome shotgun sequence.
ACCESSION   DAOQUC010000002 DAOQUC010000000
VERSION     DAOQUC010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 10707)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10707)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10707
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6926_length_10707_cov_2.876361"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..951)
                     /locus_tag="PKH07_00055"
     CDS             complement(<1..951)
                     /locus_tag="PKH07_00055"
                     /inference="COORDINATES: protein motif:HMM:NF014852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 20 zincin-like fold
                     domain-containing protein"
                     /protein_id="HNT33374.1"
                     /translation="MKRRSASIRLMQVIVSLTLCVVSVDALGDDISALMPCPRETIRL
                     EKVVEIGPRTQVVLYPNPRTADRQAAVRIQRLLSQHLGVSVPVVNCNETGYDSHSVCI
                     GQGLSNAHLTRLLAKHGLTPSVNREGFTLILSRQGIGLWGNDDDGVRHGSFVLERILG
                     TEVGVSGIPEVILKDYSVGHLRGVWVKPERSNLKALRTLVGGVFPRCRVNLMLLDLSH
                     VSWDPAVLFVDSKAVSLEEVSDLARYAQQKGISIVPCVDFLGDKRWVSAKSSDSNNAP
                     DEDSPRYLFHLKDLGVISRILESYAEIHRAIASGFFHIGFR"
     gene            complement(1263..1338)
                     /locus_tag="PKH07_00060"
     tRNA            complement(1263..1338)
                     /locus_tag="PKH07_00060"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1303..1305),aa:Ala,seq:cgc)
     gene            1744..3033
                     /gene="nusA"
                     /locus_tag="PKH07_00065"
     CDS             1744..3033
                     /gene="nusA"
                     /locus_tag="PKH07_00065"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HNT33375.1"
                     /translation="MAQMSKEVPEIIERIANERNISSKVLFEAVEAAVASAAKKMISG
                     NLQVTRVALDRDTGDFTVFAMKEVTDSITDPNLQISLQDAKEKGFNASAGEQIELDVT
                     PADFGRIAAQSAKQVVLQRLREAERDMVFDKFKDKTGDLIVGTVIRYDHGNVILDLGV
                     AEGILPRDEQPSGEHYRYGERVRAIVKDIRRSTKEPQVVLSRRSNEFLLKLFEQEVPE
                     IQDKTVTIRLVAREPGRRSKIAVMSTDSNVDAVGACVGMKGSRVQMIVQELHGERIDI
                     VEYSDDRKRLVSNSLKPAEIESVSLSEDGTRAFLVVRNDQYPLAIGKGGLNARLASRL
                     TNTEIEIISQSQIDAKDEAVKVLLLQLEGVDEQMVDRLMERMIFSYEDILRAKVDGLL
                     QVEGMTEELANSIVEQARNNILGGFSKNAQLPTEAPE"
     gene            3194..5920
                     /gene="infB"
                     /locus_tag="PKH07_00070"
     CDS             3194..5920
                     /gene="infB"
                     /locus_tag="PKH07_00070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HNT33376.1"
                     /translation="MRVYELAKKLNKETKDVLDALEAVGVSGKTHSSSITQDAVTKVE
                     AYVKAPPPPKQPAPVNVPPTTAIQEPKPGRRGPIILKPAPPPKPAPVQPPAPKPQPVE
                     EPAKIQEVSPPKVEAFKPQAPKPTPRPPEAAELPARSGTSAAIDVKKPESKKPGQKPP
                     AGRGNIKSRPLTDRLTIDVVPPVDTGKPTRQEARKSVKKKRPEDADVVDGIKKERLPS
                     RVPKEVKQKAGKVDLTAEILEEDVVLEPEKVDAQDVIIKRELQKRKKAKREAEKKEAP
                     KAPAAPQQPQISRPTPGTKKPKSRDRIRREKRDRFERAVEEERQEMERAMRTLRITEL
                     TTVSDVAENLGIPLSELIGKLMGMGLMVTKNQRLDSDTIHIIAAEYEFEVEEVDLVED
                     GFLQDYIDHSPGEERLRPPVVTVMGHVDHGKTKLLDAIRQTNVVAGESGGITQHIGAY
                     HVTLPSGQIVFLDTPGHETFTAMRAHGAMVTDIVVLVVSATEGVMPQTVEAIHHARAA
                     DVSIIVAVNKVDLPDANPDRIKQQLSEHGLLSDDWGGKTPFISISALKKTGIENLLEA
                     ILLEAEILEKKACPSRLAMGTVIESRVDRGQGSVATVLIQDGTLRTGDPFICGLYPGK
                     VRALLDDQGRAIDEAGPSMPVEILGLGGVPNSGDLFFVVEDEKIGRQLAIRLQAAQRE
                     KDIQKARPITLENLYSRVLEGQLKRLSLIVKGDVQGSVIALCDGLEKLSSEKVRLQVI
                     HSAVGPVGVADVMLAAASEALIIGFNVDMNPKVAEAAERENVEVRLYKIIYDAINDIR
                     NQMISMLDKQYAEDVIGTFEVKQVFKASKDSSVFGGVVTSGKFVRQTPIKIYRGEELV
                     FSGSLTNLKRFKEDVKEVPSGMECGMSIDGLRDIQVGDQVQCIERREVQQTL"
     gene            6088..7863
                     /locus_tag="PKH07_00075"
     CDS             6088..7863
                     /locus_tag="PKH07_00075"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HNT33377.1"
                     /translation="MRQGCLLFCLFAFLCSVCWGQVIFYQESRDSSGATNPDFYREFG
                     SGWYASGSTAKSKAEGLQGTGSRFSNAATIGDATYRFYPQYSPLFDASKMWNIYVTVP
                     GGYDSIQAENAVWTISENGINRNTAGTVPLVGFGPPDGKGPAGDKWLLVAEGVQFASE
                     EGYVEFREYPPQTNRFYADAIKYEENLTMTPNPEKTTTNTPTPTPTAAQPTITNTPLS
                     VTSECRALWVTRGNYRSESDVDNIVRNAAEHNFNVLLFQIRGNGTAFYQSQWEPWAYE
                     LTGTDPSTLGQDPGWNPLTYACLRAHEAGLQLHAYVNVFPAWQRTVPPPEGVNQLWNT
                     HRDWFMQDVNGSVMWPQGSWDYWYTFVDPGVPGVKQHLRNVILDIVQNYDVDGIHYDY
                     IRYPHEVGDWSYNQISVNRFKDLHGKDPSELPLQWNHWRRDQISDIVSSVYQDVCSVD
                     KSAVLSAAVMKSWSTAYDHAFQNYREWMSNGSLDAGILMLYIQDLNQFRGYVSEALAN
                     CSGRWIVPGLGAHNTDTNTLLDLINISRELGTQGTALFAYGNLFPGHVPNDTALALLK
                     GPFRDRATVPDMPWKQSGLGYLMTK"
     gene            complement(7882..8199)
                     /locus_tag="PKH07_00080"
     CDS             complement(7882..8199)
                     /locus_tag="PKH07_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008196523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33378.1"
                     /translation="MAKRPRCPECDSIMMEEDDGELSCPECGRVYEQEDTEEYEDDHA
                     ADDLEEEDDLDEFGDDDCEDDEDLEDLDDLEDIDDLEKDDDYEDDEEDDSSDADFEDE
                     DDR"
     gene            complement(8374..9258)
                     /locus_tag="PKH07_00085"
     CDS             complement(8374..9258)
                     /locus_tag="PKH07_00085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33379.1"
                     /translation="MMLRVNTMLSKMLSSHFGLVCAIFGIALFSVRLPCAQESEGSSG
                     EYYGADSDFFKGKDLYLRSQTTPARSHLEKYLKDNPQGRFMLEARFLLAVTETDIERA
                     KDGFWQLYRDRPEDVLGRLAYFQYGCLLLLSGQYADTSEVFEHLTLAGVGSDDGLVQR
                     RDFLPLNVMDLADFGVDGLYAASLVLAESLPAAENAVQQFASKTSSPTASERWLLLSG
                     VLADKQGRFSECQNLLSRLVATSPNSSACAAACYYALAVMRSGTGAQDGRIFADHLAK
                     AFSRSPEYRIFQRSRQAE"
     gene            complement(9248..10651)
                     /gene="miaB"
                     /locus_tag="PKH07_00090"
     CDS             complement(9248..10651)
                     /gene="miaB"
                     /locus_tag="PKH07_00090"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HNT33380.1"
                     /translation="MKFHILTYGCQMNFAESDILSRRLERAGYTPTELLEQAEIILIN
                     TCCVRATAETRAYGRLRSLRPLKESGSLRVLGVCGCLAQQDAETMLERIPHLDLVVGT
                     RALDRLADHVAKLVSEKGRISDIGDGPALTLPSEEISPASVEHSVTNAEHYPVFVSIT
                     RGCSNRCAYCVVPGVRGALESLNSSEILRQVRHLVDSGFKEVTLIGQNVNAYREGNLN
                     FSHILRKVHEVEGLKRIRFVTSHPKDLSDDIIDAVAELPKVCEYFHMPLQSGSERILK
                     LMRRGYTVDHYRALVEKIRERVVCPAGRGKVAISTDLIVGFPGETEEDFLATLQCVRE
                     IRWDGAYTFMYSARKGIEAELLPNKIPHETKLARLQILIDIQKEISHGINQERVGKIE
                     EVLVEASCPEHQSQISNASVTKEGLWRTRTRNNKIVSVQKSSLEKYLSVGDIVAVQIV
                     EARAYTLLGEVVSANDA"
BASE COUNT         2717 a         2811 c         2761 g         2418 t
ORIGIN      
        1 cctgaatcca atatggaaaa atcctgaggc aatggctcta tgaatctctg cgtaggattc
       61 aagtatcctg gagatgaccc ccagatcttt gagatggaac aaatatcgcg gggaatcttc
      121 atcgggtgca ttattgctgt cgctggactt cgcagagacc caacgcttgt caccaaggaa
      181 atccacacac ggaacaatgc ttataccctt ctgctgagcg tatcgagcta gatcagagac
      241 ttcctccaga gacactgctt tagagtcaac aaacagtact gccggatccc agctcacatg
      301 cgacaaatcc agaagcatca gattgacccg acaacgtggg aaaaccccgc cgacgagtgt
      361 tcgcaaggcc ttgagattgc tcctctctgg tttcacccag acaccccgga gatggccaac
      421 actgtaatct ttcaggatca cttcaggaat acccgaaaca ccaacttcag tgcccaggat
      481 tctctccaga acaaaagaac catggcgtac tccatcgtca tcatttcccc agagaccgat
      541 tccctgccga gaaagaatga gtgtaaaccc ttctctgttc accgagggag ttagcccgtg
      601 cttcgcaaga agccgagtca ggtgtgcatt ggatagccct tgaccaatac agaccgagtg
      661 ggaatcatat cccgtctcgt tgcagttcac gactggcaca gacaccccta gatgctgaga
      721 gagcaacctc tggattcgga cggcagcctg ccggtccgcc gttctcggat tgggatagag
      781 aacaacctgc gttctggggc caatctcgac gactttctca agacggatcg tctccctcgg
      841 gcaaggcatc agagcagaga tatcatctcc caaagcatct actgaaacaa cgcagagagt
      901 cagagaaaca atgacctgca tcagacggat gcttgctgat ctgcgcttca aaagtgcttc
      961 accacacatc atattgcgcc ttggctgaga cctttgctgt ctttctgaat caagcccttc
     1021 tccggagaaa tacttccgga agccgttgaa ccgatagata cgaacacact atatgcgcca
     1081 agatcaactc taacccagca ggtgaatcaa agcaacgttt ggaagagtca actgcagaga
     1141 ttggctgaaa tatcccgagc aggccagtgg ctgcacgtgc gtgggcaatg cctgctctca
     1201 caaccgtttg gtcattggaa gaaacagact tcttcaaagt ctggtttcct gagagatgga
     1261 gatggtggag gtgaggggga tcgaacccct ggcctgctga ttgcgaacca gccgctctcc
     1321 cagctgagct acacccccgt tgaaacgcag aagccgttct acaggtcaag agaaagatag
     1381 ccgtctcgag caactcatgt caagaacgaa aatcatcgag aggcatccgc tcccatgaat
     1441 cctataggcc ctttggaagt tctggacttc atcgagcaac agaggcaaca cgcgcttaca
     1501 atcgctacga ggcagagacg gaagcttgtg tacagccaac cggatattgt ctcatcttcc
     1561 aatagatgtg ctacttgggg gtcagaatcc gctctgctga gttttcactt gaaaatgaaa
     1621 tcgagaatat gctatagaca gagtactggt gggttttgcc cacttttttg tttgattgcg
     1681 tttatgttcc ggctgtggca aaggccttgc cggacaactg atgtgtccga agaggttatg
     1741 agtatggcac agatgagcaa ggaagtccct gaaatcattg agcgaatagc caatgaaagg
     1801 aacatctcgt ccaaggtgct ctttgaagcg gttgaggcag cagttgcctc ggcggcgaag
     1861 aagatgatca gtggcaatct tcaggtgacc cgtgtcgctc tcgaccgtga cacaggtgac
     1921 ttcacagtct tcgccatgaa ggaagtgacc gatagcatca cggatccgaa cctgcagatc
     1981 tcccttcagg atgccaagga aaagggattc aatgcctctg ccggagagca gattgaactg
     2041 gatgtgactc ctgcggattt tgggaggatc gccgcccagt ccgcaaaaca ggttgttctc
     2101 caaaggctcc gtgaagcgga gcgggatatg gttttcgaca aattcaagga caaaactggc
     2161 gatctgatcg ttggaacggt cattcgctat gatcatggaa atgtaattct tgaccttggt
     2221 gtagccgaag gaattctgcc tcgtgatgaa cagccatccg gggaacacta ccgctacggc
     2281 gaacgtgttc gagcaattgt gaaagatatt cggcgctcga ccaaagagcc tcaggtggtt
     2341 ctttctcgtc gcagcaatga gttcctgttg aaactcttcg agcaggaagt cccggagatt
     2401 caggacaaga ccgtcaccat ccggctcgtc gcacgtgaac ccggcagacg ttcgaaaatc
     2461 gcggtcatgt ccacggacag caacgtggat gccgtgggtg cttgtgtcgg catgaaaggt
     2521 tcgcgtgttc agatgatcgt gcaggaactg cacggcgagc gcatagacat tgttgagtac
     2581 agtgatgatc gcaagaggct tgtgtccaac tcactcaagc ctgccgagat cgaaagcgtt
     2641 tcgctctccg aggatggcac acgagccttt cttgttgtgc gtaacgatca gtatccactc
     2701 gcaataggaa agggtggcct gaacgctcgc ctggcatcac gtctgacaaa cacagagatc
     2761 gagatcatct cccagtctca aattgacgcc aaggacgaag cggtaaaagt gcttcttctt
     2821 cagcttgagg gcgtggacga acagatggtg gatcgcctca tggaacgtat gatcttctcc
     2881 tatgaagata tactccgtgc caaggttgac ggactccttc aggttgaagg catgacagag
     2941 gaacttgcca attccattgt ggagcaggca agaaacaata ttctcggtgg cttctccaaa
     3001 aacgcgcaac tcccaactga agcaccggag taggcttgga ccgagtgaag attcgtcttg
     3061 cattgatgag ttgccgagtg ttaatctgtc ctgagtgtct tgaaagacgg cttactgcag
     3121 caagaatttt ggggacacag gtaagagtag actgtagagc agtcatgaca agagaaaaaa
     3181 cggaggtaat cgagtgaggg tttatgagct ggccaagaag ctcaacaagg agaccaagga
     3241 tgtcctagat gcccttgagg cggtcggggt atccgggaag acccattcgt cctccatcac
     3301 ccaggatgca gtcacaaagg tcgaggctta tgtcaaggct ccgcctcccc cgaagcagcc
     3361 tgctcctgtt aacgtgcctc ccactactgc gattcaggaa ccaaaaccgg ggcgaagagg
     3421 ccctatcatc ctgaaaccag cgcctcctcc caaacctgct ccggttcagc ctcctgctcc
     3481 gaagccacag ccggttgagg aaccagccaa gattcaagag gtttctcctc cgaaagtgga
     3541 agcgttcaag cctcaggcac cgaagccaac gccgaggcca cctgaagctg ctgaacttcc
     3601 ggcaaggtct ggaacttctg ctgccataga tgtcaagaag cctgaaagca agaaaccggg
     3661 gcagaaacct cctgctggtc ggggcaacat aaagtcacgt cccttgacag atcgcctcac
     3721 tattgatgtt gtgccaccgg ttgatactgg aaaacccact cgccaagagg cgagaaagtc
     3781 tgtcaagaaa aagcgccctg aagatgcaga tgtcgttgac gggataaaga aggaacgcct
     3841 tcccagtcgt gttccaaaag aagtcaaaca gaaagcaggc aaggtagatc tgacagccga
     3901 gattcttgag gaagatgtcg tactcgaacc tgagaaggtg gatgcgcagg acgtcatcat
     3961 caagagagag ctccagaaac gcaaaaaggc caagcgtgaa gccgagaaga aggaagcacc
     4021 aaaggctcct gcggcaccgc aacagcctca gatcagccgt cccactcctg ggaccaagaa
     4081 acccaagagc cgtgatcgaa tccgacgcga gaagagagac cgttttgagc gtgccgtgga
     4141 agaggaacgt caagagatgg agcgcgccat gcgaacactt cggattacag agctcactac
     4201 tgtgtctgat gtcgctgaga accttggcat tcccctgagc gagcttatcg gcaagctgat
     4261 gggtatgggt ctgatggtga ccaagaatca gcgtttggat tccgacacga tccatatcat
     4321 cgctgcagag tatgaattcg aggtcgagga agtagacctt gtcgaggacg gcttccttca
     4381 ggactacata gatcattcgc cgggcgagga gagacttcgt cctcctgtgg ttactgttat
     4441 ggggcatgtg gaccatggaa agactaagtt gctggacgcc atacggcaga cgaacgttgt
     4501 ggccggagag tccggtggta tcacccagca tattggcgcc taccacgtta cccttccctc
     4561 tggacagatt gtatttctgg acacaccggg gcatgaaacg tttactgcga tgcgtgccca
     4621 tggtgccatg gtgacggaca ttgttgtgct tgttgtctct gccaccgagg gcgtcatgcc
     4681 tcagacggtg gaagcgattc accacgcccg agcggccgac gtttccatca ttgtcgcagt
     4741 caataaggtg gatctgcctg atgccaatcc ggacagaatc aagcagcagt tgtcagaaca
     4801 cggtcttctg tccgatgact ggggcggaaa gactcctttc atcagcatct cagctctaaa
     4861 gaagaccggt atcgaaaacc ttctggaagc aattctgctc gaagcggaaa tacttgagaa
     4921 gaaagcctgc ccttcacgac tcgcgatggg aacagtcatt gagtccaggg tggatcgcgg
     4981 acagggttct gtcgcgacgg tgcttatcca agacggaact ctgcgaacgg gagatccgtt
     5041 tatctgtggc ctgtacccag gcaaggtgcg tgcccttctg gatgaccaag gccgagcgat
     5101 tgacgaggca ggaccctcta tgcctgttga aatccttggt ttgggaggcg tgccgaactc
     5161 aggcgatctt ttcttcgttg ttgaagatga gaagatcggt cgccagttgg caatcagact
     5221 tcaggcagcc cagcgtgaga aagacattca aaaagcacgc ccaatcactc ttgagaatct
     5281 ttattcgaga gtgctcgaag gacagcttaa gcgcttgtcc ttgattgtca aaggtgatgt
     5341 tcagggttca gtgattgctc tctgcgatgg cttggagaag ctgagttcgg agaaagtccg
     5401 gctccaggtt atccacagcg cagtgggtcc cgtgggagtg gctgatgtca tgcttgccgc
     5461 agcctcggaa gcattgatca tcgggttcaa cgttgatatg aacccaaaag ttgctgaagc
     5521 ggcagaacgc gagaatgttg aggttcgcct ttacaagatt atctacgatg ccatcaacga
     5581 tattcgaaat cagatgattt cgatgttgga caaacagtac gcggaagatg ttattggaac
     5641 ctttgaagta aagcaggtct tcaaggcttc caaggatagc tcggttttcg gtggtgttgt
     5701 aacatcgggc aagttcgtcc gccagacacc gattaagatt taccgtggcg aagaattggt
     5761 cttttccggt tcgttgacga acctgaagcg tttcaaagag gacgtcaaag aggtgccatc
     5821 cggaatggag tgtggcatgt ccattgacgg tctgcgagac attcaggtgg gcgaccaagt
     5881 ccagtgcatc gaaagacgcg aagtccagca gaccctttag ggacaacaaa cccaacatca
     5941 gcgaacgctt cgcagactcg ctctgtctga tgcactgcac ggacggagta tgcgtccttc
     6001 acttttctat ccaatcagac tctatcgtgg tcggaggaat cgtctgatca ccagcaaaca
     6061 gggtcatgaa gaataggagt taccagtatg cgtcaaggct gtttgttgtt ctgcctgttt
     6121 gcttttcttt gctcggtctg ctggggacaa gtgatcttct atcaagagtc cagggactcg
     6181 agcggtgcca caaatcccga tttctacagg gaattcggaa gtggctggta tgcctcagga
     6241 tcaaccgcga agagcaaagc cgaagggctt cagggaaccg gctctcgctt ttcgaacgct
     6301 gcaaccatcg gtgatgccac ttaccggttc tacccgcaat acagtcctct atttgatgcg
     6361 tcgaagatgt ggaacatcta tgtcacggtg ccaggaggat atgactcgat ccaggccgaa
     6421 aatgctgtct ggactatcag tgaaaacgga atcaaccgaa acaccgctgg gacggtgccc
     6481 ttggtcggct tcggaccacc cgacggcaag gggcctgccg gcgacaaatg gctcctggtc
     6541 gctgagggcg tgcagttcgc ctccgaggaa ggatatgttg agttcagaga gtatccgccg
     6601 cagacaaatc gcttctatgc agacgcgatc aagtatgagg agaatctgac catgaccccc
     6661 aaccctgaga aaaccaccac aaacactccg accccgacac caacagcggc gcaacctaca
     6721 ataacaaaca ccccgctcag tgttacgagt gagtgtcgtg cgctttgggt cacccgaggc
     6781 aactaccgtt ccgagtccga cgttgacaat atcgtcagaa acgcggccga acacaatttc
     6841 aatgtactcc tctttcagat ccgagggaac ggcacggcat tttaccagtc gcagtgggaa
     6901 ccctgggctt acgagttgac cggcaccgat ccttccacgc tgggtcagga tccgggatgg
     6961 aatcccctca cgtatgcatg tttgagagcc cacgaagcag gcctacagtt gcacgcctat
     7021 gtgaatgtct tccctgcgtg gcagcggact gtgcccccgc cagaaggagt gaaccaactc
     7081 tggaacactc atcgagattg gttcatgcaa gacgtcaatg gctctgtcat gtggccacaa
     7141 gggtcatggg attactggta cacctttgtc gatcccgggg tacccggtgt aaagcagcat
     7201 cttcgaaatg tcatcttgga catcgttcag aactatgacg tggatggcat ccactatgac
     7261 tacattcgat atccccatga ggtcggagac tggagttaca atcagatatc cgtaaacaga
     7321 ttcaaagatc ttcacggaaa ggatccttcg gaactaccac ttcagtggaa tcattggcgc
     7381 cgcgatcaaa tatcagacat tgtttcttcc gtctatcagg acgtatgcag tgttgacaag
     7441 agtgcagtct tgtccgccgc ggtgatgaag agttggtcaa ccgcctatga ccacgctttt
     7501 cagaactatc gagagtggat gagcaacggc tcattggatg ctgggattct catgctctac
     7561 attcaggatc tgaatcagtt ccgaggctac gtcagtgagg ctctcgcaaa ttgctctgga
     7621 cgttggattg taccgggtct gggagcacac aatacggaca ccaatacgct actggattta
     7681 atcaacatca gccgagaact cggaacacaa gggactgcac tctttgccta cggaaatctg
     7741 ttcccgggtc atgttcccaa cgatacggct ctggctttgc tgaagggtcc gtttagagat
     7801 cgtgcgactg tacctgatat gccatggaag cagtccggac tgggttatct gatgacaaag
     7861 taggcattga gcccttctct ctcagcgatc atcctcgtct tcgaagtccg catcggagga
     7921 atcgtcctcc tcgtcgtctt cgtagtcgtc gtccttctcc agatcgtcaa tatcttctag
     7981 atcgtcgaga tcttcgaggt cttcatcgtc ctcacagtcg tcatcaccaa actcatccaa
     8041 atcgtcttct tcctcaagat cgtctgcggc gtgatcgtct tcgtattcct cagtatcttc
     8101 ttgttcgtaa acgcgaccac actcaggaca ggagagttcg ccatcatcct cttccatcat
     8161 aattgaatca cattctggac aacgaggtct cttagccatt gtcaattcac ctcccagtga
     8221 agggtgcgga gtaccatccc tggttttgtg ttaagtcagt cttagtcgtc tcgcatcaaa
     8281 ttggttccgg cgggtatata ccccaaaaaa accttcgaat gcaagtcttt ttttctcatc
     8341 tccctgaccc gcaacgatga tgggaagacc ttgtcattct gcctgtcgag atcgctgaaa
     8401 gattcgatac tcagggcttc tggaaaacgc ttttgcaaga tggtctgcaa agattcgccc
     8461 gtcctgtgcg cctgttcctg agcgcatcac ggcaagggcg tagtagcaag ccgcagcaca
     8521 cgcacttgag ttcggacttg tggcaacaag ccgactcaga agattctggc actcggaaaa
     8581 acggccttgc ttgtcggcga gcacacctga gaggagaagc caacgctcag aagcagtcgg
     8641 actcgatgtc ttggaggcaa actgttgtac tgcattctcg gctgccggaa ggctctcggc
     8701 aagaaccaat gacgccgcat ataaaccgtc aactccaaaa tccgcaagat ccatcacatt
     8761 caaaggaagg aaatccctcc gctgaaccag cccatcatcc gatccgactc ccgccaatgt
     8821 cagatgctcg aaaacctctg acgtgtccgc atactgccca ctcagcagca gaagacaacc
     8881 atactggaaa taggccagtc gcccaaggac atcttcaggc cgatcccggt acaattgcca
     8941 aaaaccatcc ttcgcacgct caatgtcggt ctccgttaca gccagcagga atctggcttc
     9001 aagcatgaaa cgtccctgcg gattgtcctt gagatacttc tcgaggtgtg agcgggctgg
     9061 cgttgtttga gagcgcaggt acagatcctt gcctttgaaa aagtctgaat cggcgccata
     9121 gtactctccc gaagaccctt ccgattcctg agcacaaggc agcctcactg aaaacaaagc
     9181 gatcccaaag atcgcacaga cgagtccgaa gtgactcgag agcatcttac tcaacatcgt
     9241 gttcacccta agcatcattg gccgatacca cctctccaag gagagtatac gctcgcgcct
     9301 caacaatctg aactgcaacg atatctccaa cactcagata tttctcaaga gaagatttct
     9361 gaacagagac gattttgtta ttgcgcgttc gcgttcgcca gagtccctct ttcgtcacag
     9421 acgcgttaga gatttgtgac tggtgctccg gacaggaggc ctcgaccaga acttcttcga
     9481 tcttgcccac acgttcctgg ttgatcccat ggctgatctc tttctgaata tcgatcaata
     9541 tctgcaagcg ggcaagtttg gtctcgtgtg ggatcttgtt cggaagcagt tcagcctcta
     9601 tccccttgcg cgctgaatac atgaacgtgt aagcaccgtc ccagcgaatc tcccgaacac
     9661 actgcagagt ggccagaaag tcctcttccg tctcgcctgg aaacccaacg atcagatcgg
     9721 tgctgatggc cactttgcct ctgcctgcag ggcagacaac gcgttcacga attttctcca
     9781 caagcgccct atagtggtca accgtatagc cacggcgcat cagtttgaga atccgttccg
     9841 aaccggactg aagaggcatg tgaaagtatt cgcacacttt cggcagttcg gccacggcat
     9901 caatgatatc atcggataag tccttggggt gtgaagtgac aaatcgaatt cgcttcaaac
     9961 cctcaacttc atgtactttt ctaagtatgt gcgagaagtt gagattccct tccctgtagg
    10021 cattcacatt ctgcccgatc aaagtgactt ccttgaaacc cgaatcaacg agatgtcgaa
    10081 cttgtcggag aatctcgctt gaattgaggc tctccagtgc tccccgaacc cccggcacga
    10141 cacaataggc acaacggtta ctgcagcctc ttgttataga aacaaagaca ggatagtgtt
    10201 ccgcattggt cacagagtgt tccacagacg caggactgat ctcttctgat ggaagggtca
    10261 gagctggccc atcaccaata tcggaaattc ggcctttctc cgaaaccaac ttggcaacgt
    10321 ggtctgccag gcgatccaga gcgcgtgtcc ccacaacaag atccagatgg ggaattcgtt
    10381 ccaacatcgt ctcagcatcc tgttgagcca gacaaccaca gacccccaat actctgagag
    10441 accctgattc cttgagaggc ctgagcgatc gaagtctgcc ataggcacga gtctctgctg
    10501 tcgctcgaac acagcaggtg ttgatgagga taatttcagc ctgctccagc aactctgttg
    10561 gagtgtatcc agctcgttca aggcgtcgtg aaaggatatc agattcggca aagttcatct
    10621 gacatccata ggtgagtatg tggaacttca tcagatcagc atcccatcaa tatcgcggag
    10681 gttgccccat ggctcgcatc acgactg
//
LOCUS       DAOQUC010000003         9618 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_7929_length_9618_cov_3.510928, whole genome shotgun sequence.
ACCESSION   DAOQUC010000003 DAOQUC010000000
VERSION     DAOQUC010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9618
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7929_length_9618_cov_3.510928"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(121..1143)
                     /locus_tag="PKH07_00095"
     CDS             complement(121..1143)
                     /locus_tag="PKH07_00095"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HNT33381.1"
                     /translation="MNRIAVLGLGSWGLTLARLLHNKGFEVAGWEFDPQAARQLQECR
                     ELKGKLPGVQLPEGVQVSTDMSDIVSGTDLIVFAVPSQTMRATCETLKRTEAAQRQIL
                     VSVSKGLEQGTLKTMTEVIAEATQLKQPERLVALSGPSHAEEVSRDIPTSVVVAGCSE
                     LVTRQVQETFMTERFRIYTNDDLSGVELAAALKNVIAIAAGISDGLGFGDNAKAALIA
                     RGLSEMMRLGIRMGAKPYTFTGLAGLGDLVATCTSRHSRNRNFGELIAKGLSKEQALQ
                     RIGMVVEGLETARSILLLQERCGVEMPISVEVYRVLLQGKAPGDAVRDLMLRDAKPEM
                     EVQLYQ"
     gene            1537..2289
                     /locus_tag="PKH07_00100"
     CDS             1537..2289
                     /locus_tag="PKH07_00100"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HNT33382.1"
                     /translation="MRYAVISDIHANLEALSVVLEDIRSQNCDATLCLGDVVGYGANP
                     NECVQIVFEQCRPILEVGLPIVKGNHDEAVTSGNARDFNDAAAAAARWTRETLECENA
                     KRIADLPMTVMLDDVFLVHASPFEPERWHYVLSVVSAFEAIDHFPESTRVCFIGHSHC
                     PSVMCQNERGDVLRVSGSLVSLEDQKRYLINVGSVGQPRDSDPRASYAVYDTESKTVE
                     IRRLQYDIPTAQRKIEDAGLPGSLARRLAFGR"
     gene            2389..3687
                     /locus_tag="PKH07_00105"
     CDS             2389..3687
                     /locus_tag="PKH07_00105"
                     /inference="COORDINATES: protein motif:HMM:NF005560.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNT33383.1"
                     /translation="MKIAVVGGGISGLVAGFDLAKAGHEITVFEKDHVLGGLCACFRL
                     EGTYLEKYYHHFFTSDQAIIDLFEQLGLSPLVEWRTSLTGVYYDGHAYPFGGPVDLLR
                     FKPLPMADRLRLGFAIMRLRKIDDWRPLEEITAKDFIVREMGEKVYRVLWEPLLRSKF
                     GDKAESVAAVWFWGKIKLRGSTRTKSGSKELLGYLRGGLGLAIDELAKQITERGGRVL
                     TSSPVERIAVDGDKIQVTSLQGVDHFDRLVFTGAPSLFASLCDDLSDDYRQRLQSVHY
                     QGTTCLVLKTKQSLSNIYWLNINDLNMPFVGIIEHTNYRSPQEYGGYRVIYVTRYLDP
                     NNRLYSLDADELFEEYKPHIKRIFPHFDPAAVQERFIFKDAFTQPVIRLQYSRQIPDF
                     TTPLPGVCLASMAQIYPEDRGSNYGVVMGHKASEWIKKSL"
     gene            3802..5043
                     /locus_tag="PKH07_00110"
     CDS             3802..5043
                     /locus_tag="PKH07_00110"
                     /inference="COORDINATES: protein motif:HMM:NF025690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HNT33384.1"
                     /translation="MNAPALQGNLSTFSLEDVLKFVEEKGHAGVLEITQDTGNLLVYF
                     EQGQVVCFSGLLDGSKIGRLLFRRAVIGKQDYERLSQVQNADFFKILSEVFTRVDPSL
                     KGILTECLRLQEREETVVLFSLKEGNFQFTPQQVDCPKALKCRTPIPQFLETGRQLSA
                     QWKQVQTILSNQNLYPQIPDLDQVALEECKPGLDLWLVLSVIDGRRTVAEISKVSPWG
                     RFETLLGLVRFMQKKLVVLSDKPVPKPALVSRSTVSVPQETGGFLASFRGKEQHSYQP
                     RTKAGVAALVVEGFLESLNSRKEIQLDATAVKSAWAEALVLFPMADLLNVSGEGADVS
                     LLEDLLDTWADSESTRDVVESSAEALKRFSESLLRIASTQMGDKKAQSLYAKVCSGLA
                     KRTDLPITAEEMRALGLDKQA"
     gene            5056..5955
                     /locus_tag="PKH07_00115"
     CDS             5056..5955
                     /locus_tag="PKH07_00115"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNT33385.1"
                     /translation="MPGETVLLIDDSEARLDMMSRLVRRNGFEPLQAQNGLAALTLPE
                     DKKVALIIINTGLMQPDGEELAERLRRSSRAWYLPILLLVPSEKQHSGEQQPLFGADS
                     FINFPCSAADLARKMDEVLRDKKARDFAEERLVEITRQHISDAVDRVAEQELSTKSAE
                     VISDLSAGIVDLVSIEAKKTMDQKVQELVAGAFAEQVRAIVEDVARTVLAEESENIIT
                     TLVASVVDQQVGQILGKLETDELPNIARRAVEKVVEVAVKEKVAQITMETHAETVQKL
                     VSQISEVVERTARSIIPKILGKQ"
     gene            6106..7629
                     /locus_tag="PKH07_00120"
     CDS             6106..7629
                     /locus_tag="PKH07_00120"
                     /inference="COORDINATES: protein motif:HMM:NF023802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HNT33386.1"
                     /translation="MLVLALLGLSQTAEADKVLDVPYMRQPDGSTCMPTSLLMTLEYF
                     GKDKLSEQRIFELHRRTRVNRLNIPAILEEYGMHGDASYSPYWTFEDIKKDIDDGLPV
                     ILGTDISDAGHIIVAVGYTDDGRVIVNDPGYQKTGKVVKTLEEVIFDGGGVRFREVPK
                     GPYQGEWVAHRRIGWVSANTRHEIWFEYRNRGTETWPAGKVELRPTGSLPSDTFYDEE
                     TWVSPTCVVRLDRDVKPGETHRFTFSVRIPDVTMQKEFCQPFHLFLTPDTPFSYPRDQ
                     DISIEIRVWPKYTLPLRDRFPASGPELPWIARFQPPQMVQRKDDPLRDGAVLRVQDTL
                     EGGYDSIRIGSLEVGDYRVRALVYCAYRPDIEDGTGFERVGLFARDRYRGEFEKSKIG
                     NCYLMTHDSNNGRIICGKVQAGEITDFLEKPVLLVKTGWHEFRMDCQGNRIRYFLDRK
                     LIADVQDDFRNEGQCGIGYHEFFADNAHAHGALVARFSMEPMRKRGLSRLLFWRKAK"
     gene            7626..>9618
                     /locus_tag="PKH07_00125"
     CDS             7626..>9618
                     /locus_tag="PKH07_00125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33387.1"
                     /translation="MKIKRRYLVLGVLFLLLAVIITAATLSVERIAKKAVSSALASKN
                     LDGGFETLDIGLLRGVCAVGGLDLNTKDGQPLLELKRVQANVNLFSLLRKKISVDGLV
                     VEGMKVNVQIDQQGKINWLNLLEKAESVEEATKEPSSASGSVAVRNVTLQDIVLEIED
                     LRPDTMLKSVSVALGNLDARLSRGKISLRNLKIESSDPQPYFGVDSIEMMGKFLVDSG
                     VSIRVDSVTVAGVKGRNRLQDNGKFVVQQWAEDIASLNGLMSDKPVQPKPASGEKSAE
                     MPTRIQLADLDYALVYAGSGNKPLVERIQIASLALALKDGKFDLEGLDWSQSLDKGVG
                     DTVVHLGSLSLTAELLKSPLTVEGAQIKDLSLSLRKGVDAPFDIQQRLTTLQQKFPQK
                     PQEKKETAPTEKAPFSVRGSINLDSVRLRLVAQETEKILVNQIVQLGSMTFDPAKGFF
                     GLNGLVVSDDKGNMETPSLRISSVEVKGNLDVPLPEKLQINQVSVNAPSTSLACSSDG
                     KIDLLERMDVLTRTAPKPKTPPAAKKSESSLNLELKQLAVSDLSFSLVHQVKSEQQVT
                     FRMHQGVVNLTDLVLPKGGSGTTRLSLKGAFVQPSAGSVDLSSEIPQQSFADDFVAKF
                     LMSLSDITPLKPYYEESMPFELKAGGFAFQTEGQCKARNL"
BASE COUNT         2272 a         2437 c         2599 g         2310 t
ORIGIN      
        1 gtgcggctcc acgcccgtat actcgcttac ctcgctgatc gaatagtaga tcttgcgcgg
       61 gatctccgac aaaggaatcg ttctcggtgt tgacatgatt tcgttcgttc catttcctct
      121 ctattgatat agctgcactt ccatctccgg tttcgcgtcc cgaagcatca ggtcgcgaac
      181 cgcgtcccca ggagccttcc cctgcaacaa tacccggtac acctcgaccg agattggcat
      241 ctccactcca caacgctcct gaagcaataa aatggatcgg gcggtttcaa gcccctcaac
      301 caccattccg atccgctgga gtgcttgttc tttcgagaga cccttggcaa taagctctcc
      361 aaagtttcgg tttcgactgt gccgactggt acaggtcgcc accagatcgc ccaaacctgc
      421 cagacctgtg aacgtgtacg gcttggctcc catccgaatc ccaaggcgca tcatctccga
      481 tagcccccgc gcgatgagtg ccgccttggc gttgtcgcca aatccaagac cgtctgagat
      541 tccggcagca atagcgatca cgttcttcag tgcagcagcc agttcaaccc ctgaaagatc
      601 atcattggta taaatccgaa agcgttccgt catgaaggtc tcctgaacct gtcgggtgac
      661 caattccgaa caccctgcca ccacaacgga tgtcggaatg tctctggaaa cctcctccgc
      721 atgacttgga cccgagagtg ctaccagtcg ctccggctgc ttcagttgtg ttgcctctgc
      781 tatcacctcc gtcatggttt tgagtgtccc ttgctccaat cccttcgaga cactcaccag
      841 aatctgccgc tgtgcggctt ctgttcgctt caacgtttca caagtagccc gcatcgtctg
      901 tgacggcacc gcaaagacga tcaaatcggt gcccgacacg atatcggaca tatccgtgct
      961 gacctgaact ccctcgggaa gctgcactcc aggcaattta cccttcagct cccgacactc
     1021 ctggagttgt cgagccgcct gcggatcaaa ctcccagccg gcaacctcga agcccttgtt
     1081 atgtagtagt ctcgccagag tcaggcccca gctgcctaac ccaagcacgg ctattcggtt
     1141 cacttgctcc aacctatccc atttcttcta cataggtcta ttatagaaat ctcgtgcttg
     1201 agatcaaggg atttttctat agtaaaaaca ataagttaga ctattacgca atatcttgag
     1261 tccccagaac acagaatatc aatatgtttg aatcaatctg aagtcttcga attcaactct
     1321 tttgaaaaca aatgaaaaac accacaggga catgttcaat gcctgcgcca tgcatgggct
     1381 aagagtcttg ggttgtcttg ggttgttgag gcaagacgga tggcttcgct acataactga
     1441 gccggtactg cgatgcagta cttgtctttt ctgccagtct atgagattgt ttgaattggc
     1501 aacaaggacg gaatccgcct gtgatgggaa ttggaaatgc gctatgcggt gatctccgac
     1561 attcacgcga atctcgaagc actttctgtg gtgcttgagg acatccgaag ccaaaactgt
     1621 gacgcaactc tttgccttgg ggatgttgtt ggctatggtg ctaatcccaa tgaatgtgtt
     1681 caaattgtct tcgagcaatg tcgcccgatt ttggaagttg gtctgccgat cgtaaaaggg
     1741 aaccatgacg aggcggtaac atcaggcaac gctagagatt tcaatgacgc cgccgcggcg
     1801 gctgcccgct ggacaagaga gactctggag tgtgaaaatg ccaaacggat cgccgatctt
     1861 ccaatgactg tgatgctgga tgatgtgttt ctggtgcacg cgagcccctt cgagcccgaa
     1921 agatggcatt atgtcctcag cgttgtcagc gcttttgagg ccattgacca ctttccagaa
     1981 agcacgagag tctgtttcat cggacacagc cactgcccct ctgtcatgtg ccagaatgag
     2041 cgcggcgatg tactgcgtgt ttccggttcg ttggtaagtc tcgaagatca gaagcggtat
     2101 ctaatcaatg tgggaagtgt cggtcagcct cgcgactcgg atccgagagc cagctatgcg
     2161 gtttacgata cggagagcaa gacagtcgag attcgtcggc tgcaatatga cataccgact
     2221 gctcaacgca aaatcgagga cgcgggactt cccgggagcc tggcaagacg acttgccttt
     2281 ggccgctgag attctggatt tgacgatgcg gggaaagaaa ggcaacatgg aagacttgga
     2341 tgcgttgcaa tgacaagcac gtgaaatctg gcagtgagag gtgagaatat gaagattgcc
     2401 gtcgtcggtg gtggaatctc cggtctggtt gcaggctttg acctggccaa ggcaggtcac
     2461 gagatcacgg tcttcgaaaa ggatcatgta ttgggtggat tgtgcgcctg ttttcggctt
     2521 gagggcactt atctcgagaa atactatcat cacttcttta caagcgatca ggcgataata
     2581 gatctcttcg agcagctcgg attgagccct ttggtcgaat ggcgaacatc cctgacagga
     2641 gtctactatg acggtcatgc ctatcctttc ggagggccgg tagatctgct gcgattcaaa
     2701 ccgctgccga tggcagaccg attgcgtttg ggtttcgcca tcatgcggct tcgcaagatt
     2761 gatgactgga gacctctgga agagatcacg gcaaaggact ttatcgttcg cgagatgggc
     2821 gaaaaggttt accgggttct ctgggagccc ctgcttcgct ccaaattcgg cgacaaggca
     2881 gaatcggtcg cggcggtttg gttctggggc aagatcaaac tgcgtggcag cacgagaacc
     2941 aagtctggca gtaaggaact tctgggctat cttcgcggag gacttgggct tgccattgac
     3001 gagttggcaa aacagattac tgagcgaggc ggaagagtcc tgacttcgag tcctgtcgaa
     3061 cgcatcgctg tggacggaga caagattcag gtcacgtcgc ttcagggcgt ggatcatttt
     3121 gaccgattgg tttttaccgg tgctccttct ctgtttgcca gtctgtgtga cgatctttcc
     3181 gacgattacc gacaacggct tcagagtgtt cattaccagg gaacaacctg tctggttctg
     3241 aagacaaaac agagtctctc caatatctac tggctgaaca tcaatgatct gaacatgcct
     3301 ttcgtcggaa tcattgaaca caccaactac cgttctccgc aagagtatgg tggctatcga
     3361 gtgatctatg tgactcggta cctggatccc aacaatcgcc tttattccct tgatgccgat
     3421 gaactgtttg aggaatacaa gcctcacatc aagcgcatct ttccgcattt cgatcctgct
     3481 gccgtccagg agcggttcat cttcaaggat gccttcacac aaccggtcat caggctgcaa
     3541 tactcaaggc agattccaga tttcactacc ccacttccag gcgtatgcct cgcttcgatg
     3601 gcgcagatct atcctgaaga tcgaggctcc aactatggag tggtcatggg acacaaggcc
     3661 tccgaatgga tcaagaagag tctgtgagca cagagttggc aggccatcct ctgttgacaa
     3721 tgggagcgaa acggacttaa gatacttctc aatgtctgcc aacttaactg tcgtccctag
     3781 actgtgacgg ggtttttgaa gatgaatgca cctgcactac aaggcaatct gagtaccttc
     3841 tcgcttgagg atgtactcaa attcgttgag gaaaaaggcc atgccggagt cttggagatc
     3901 actcaagaca ctggaaatct gcttgtctat ttcgaacagg gacaggtcgt gtgctttagt
     3961 gggctgttgg atggatccaa gataggacgt ttgctttttc gtcgcgcagt gatcggcaag
     4021 caggactatg agcggttgtc ccaggttcag aatgccgatt ttttcaagat tctctcagag
     4081 gtcttcacac gcgttgatcc ttcgctgaag ggcatcctta ccgagtgcct tcggctgcag
     4141 gaacgggaag agacggtcgt gctcttctcc ctcaaagaag gcaattttca attcacaccg
     4201 cagcaggttg actgtcccaa ggctctcaaa tgcaggactc ccattcccca gtttcttgaa
     4261 acaggcagac aactgagcgc gcaatggaag caggttcaga ccatcctctc caatcaaaat
     4321 ctgtatcctc agattcccga tctggatcag gtcgccttgg aggaatgcaa accgggattg
     4381 gatctgtggc ttgtcctttc ggtcattgac ggccgtcgaa cggttgcaga aatttcgaag
     4441 gtttcccctt ggggacgctt cgagaccctt ctggggttgg ttcgattcat gcaaaagaaa
     4501 ctcgtcgtgc tcagcgacaa gcccgtgccg aaaccggcgc tggtcagccg ctcgactgta
     4561 tcggtgcctc aggaaaccgg aggatttctc gcttcgtttc gtggcaagga acaacattcc
     4621 tatcagccgc gcaccaaggc aggcgtggca gcgcttgtag tcgaaggttt tctggagagt
     4681 ttgaactcgc gcaaagaaat acaactggac gccactgcag tgaagtcagc ttgggcagag
     4741 gcattggtgc ttttcccaat ggctgatctg ttgaacgtct ccggcgaagg ggcagatgtc
     4801 tctttgctgg aagacctgct cgacacctgg gcggattcgg aatcaacacg cgatgttgtg
     4861 gagagttctg cagaagcact caagcgtttc agtgagagcc tgcttcgaat tgcttccacg
     4921 cagatgggtg acaagaaggc acagtccctt tacgccaagg tctgctccgg acttgccaag
     4981 aggacggatc tccccattac ggcggaggag atgcgggctt tgggactgga caaacaagca
     5041 taaaggaata attctatgcc tggtgaaacg gtactgctca ttgatgacag cgaagcacgt
     5101 ttggatatga tgagtcgcct tgtgcgacgt aacggttttg aacctctgca ggcacaaaac
     5161 ggtcttgccg ctctcactct ccctgaagac aagaaggtcg ccctgatcat catcaacaca
     5221 ggtttgatgc aacccgatgg tgaggagttg gcggaacgat tgcgccggag ttcaagagcc
     5281 tggtatcttc ccattctgct ccttgtgcct tcggagaaac agcactcggg agaacaacag
     5341 cccctttttg gcgcagacag cttcatcaac tttccctgct ccgcggcaga cttggctcgc
     5401 aaaatggatg aagtcttgcg ggacaagaag gctcgggatt ttgccgaaga gcgactggta
     5461 gagattaccc gccaacatat cagcgatgca gttgaccgtg tggcggagca ggaacttagt
     5521 accaagtccg ctgaggtgat ctccgatcta tccgccggta ttgttgattt ggtcagcatc
     5581 gaagccaaga agacgatgga tcaaaaagtg caggaactgg tggcgggcgc ttttgcggaa
     5641 caggttcgtg ccatcgttga ggatgtcgcc cgaacggtgc ttgcagagga atccgagaac
     5701 atcatcacga cactcgtcgc ttccgtggtg gatcagcagg tcggtcagat tcttggcaaa
     5761 ttggaaaccg atgaactgcc taacatcgcg cgcagagcgg ttgagaaggt ggtcgaagtg
     5821 gctgtcaagg agaaggtggc tcagatcacg atggagactc acgcggagac agtccagaaa
     5881 ctcgtcagcc agatttctga agtggttgaa agaaccgcac gaagcattat tcccaagata
     5941 cttggaaaac agtgatctgt ccgcatctgc atatctctct gtcgaaatga atgagcggac
     6001 gacaatcgta ttggacgttc aggatagcaa tcgttaggga ggttatctga tttgatacac
     6061 tgggactcta cttctactca gacaaaaggt ctttggatct gcttggtgct tgttctcgct
     6121 cttctgggtc tttcgcagac ggccgaagca gacaaggtac tggatgtccc ttacatgaga
     6181 cagcctgacg gctccacctg tatgccgacg tcattgctga tgacactgga gtacttcggc
     6241 aaggacaaac tcagtgagca acggatattc gaactgcatc ggcgcacccg cgtcaaccga
     6301 ctgaacattc ccgcaatcct tgaggaatac gggatgcacg gggacgccag ttactctccc
     6361 tattggacgt ttgaagacat caagaaggac attgatgatg ggcttccggt cattctgggc
     6421 acagacatct ccgacgcggg gcacatcatt gtcgcggtgg gttataccga tgacggtcgt
     6481 gtcatcgtga acgatcccgg atatcagaaa actggaaaag ttgtcaagac cttagaggaa
     6541 gtgatttttg atggaggcgg tgttcgcttt cgcgaagtcc caaagggacc gtatcaaggt
     6601 gagtgggtgg cgcatcgcag gatcggttgg gtctccgcca acacacgcca tgaaatctgg
     6661 tttgaatacc gaaatcgcgg gacggagacc tggcctgccg gaaaggtcga actgcgtcca
     6721 acgggttcac tgccgagcga caccttctac gacgaggaga catgggtttc tccgacatgt
     6781 gtggtgcgac tggatcgtga tgtgaagccg ggagagacac atcgcttcac gttctccgtc
     6841 cgtattccag atgtcacaat gcaaaaggag ttctgccagc cttttcacct ctttttgacg
     6901 cccgatactc ccttcagcta tccaagggat caggacatca gcatcgagat cagagtctgg
     6961 ccaaaataca ctctgccgct ccgagatcgc ttccccgcat ccggtcccga gctgccgtgg
     7021 attgcccgat ttcaaccacc acagatggtg cagcgcaaag atgatccgct gcgcgacggt
     7081 gccgtccttc gcgttcaaga cacactggag ggtggatacg actccatccg cattgggagt
     7141 ctggaagtgg gagattaccg agtgcgcgcc ctggtgtact gtgcctatcg tccggacatt
     7201 gaggacggaa cgggatttga acgagtaggt ctatttgccc gggatcggta ccggggagaa
     7261 tttgagaaaa gcaagattgg caactgttat ctgatgacgc atgattccaa caatggaaga
     7321 atcatctgtg gcaaggttca ggctggagag atcactgatt tccttgaaaa acccgtgttg
     7381 cttgtaaaga caggctggca tgagtttcga atggactgtc aaggaaaccg gattcgatac
     7441 tttcttgacc ggaaactcat cgcggatgtg caagatgatt tcagaaacga agggcagtgc
     7501 ggcatcggtt accatgagtt ctttgctgac aatgcccatg cccatggcgc actggtggct
     7561 cgcttctcga tggagccaat gaggaagagg ggtctctcaa gacttctctt ctggagaaaa
     7621 gcaaaatgaa gatcaagcgt cgctatctag tccttggggt tctgttcctg ttgctcgccg
     7681 tcatcatcac tgctgccacc ctatcggtag aacgcattgc aaagaaagcc gtttcttcag
     7741 ccttggcaag caaaaatctc gatgggggct ttgaaaccct tgatatcggt cttctgcggg
     7801 gtgtctgtgc cgtcggcgga ctcgatctca acacaaagga cggacagcct cttcttgaat
     7861 tgaagcgggt acaggcgaat gtcaacctct tcagccttct tcgtaagaag atctccgtag
     7921 acgggcttgt tgtcgaaggt atgaaagtca acgtccagat cgatcaacag ggaaagatca
     7981 actggctgaa tctgttggaa aaggcggaat cggttgagga ggcaaccaag gagccgtctt
     8041 cggcctctgg ctcggtcgcg gtcaggaatg tgactttgca ggatattgtg cttgagattg
     8101 aggacttgcg tccggacacg atgttgaagt ctgtctcggt cgcactcggc aatttggacg
     8161 ctcgtctttc gcgtggcaag atctccttga ggaatttgaa gatcgaatca tccgatccgc
     8221 aaccatactt tggagttgac tccattgaga tgatggggaa gttcctggtg gattcaggtg
     8281 tatcgatccg agttgattct gtgaccgtcg ccggcgtcaa agggagaaac cgactgcagg
     8341 acaatggaaa gttcgttgtc cagcaatggg ctgaggatat agcatccttg aacggtctga
     8401 tgtcggacaa gccggtgcaa cctaagcctg catcggggga aaaatctgca gaaatgccaa
     8461 cgagaatcca gttggctgat ttggactatg ccttggtcta tgcgggcagt ggcaacaagc
     8521 ctctcgtcga gagaatccag atcgcgtcgc ttgccctggc gctcaaggac ggcaaattcg
     8581 atctggaggg gctggactgg agtcagagtc tggacaaggg cgttggggat accgtcgtgc
     8641 atcttggtag tctttcgttg acagctgagc tcctcaagtc gcctttgacc gttgaggggg
     8701 cgcagatcaa ggacttgagt ctctctcttc gcaagggagt ggatgctccc tttgacattc
     8761 aacagcgcct gaccacattg cagcagaaat tcccacagaa accacaggag aaaaaagaaa
     8821 ctgctccaac ggagaaggct ccattcagcg tcaggggttc gatcaatctg gactcggttc
     8881 gccttcgcct tgtcgcacag gagacagaaa agatccttgt caatcagatc gtccaacttg
     8941 gttccatgac ctttgatcca gcgaagggat ttttcggact caacggactt gttgtttcag
     9001 atgacaaagg caacatggag actccctctc tgagaatctc gtctgttgag gtgaaaggta
     9061 atctggacgt tccgttgccg gagaagctgc agattaacca ggtgtctgtc aacgcaccaa
     9121 gcaccagtct ggcgtgttcc agcgatggaa agattgatct tcttgagcgg atggacgttc
     9181 tcactcgaac agcacccaag ccaaagaccc ctccagcagc gaagaagtct gagtcctctc
     9241 tgaatctcga actcaagcag ctggccgtca gtgatctttc tttttctctt gtccatcaag
     9301 tcaagtccga acagcaggta accttccgga tgcaccaggg agtagtgaac ctgacggatc
     9361 ttgtccttcc caagggagga tcgggcacga cccgactttc gctgaaaggg gcttttgtac
     9421 aaccatctgc gggcagtgtg gatctttcct cagagattcc ccagcagtct tttgccgacg
     9481 atttcgtcgc aaagtttctc atgagtttgt ctgacatcac tccgctgaag ccctactacg
     9541 aggagtccat gccgtttgag ctgaaggccg gtggttttgc ctttcagact gagggacaat
     9601 gcaaggccag gaatctgg
//
LOCUS       DAOQUC010000004         9028 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_8579_length_9028_cov_3.380475, whole genome shotgun sequence.
ACCESSION   DAOQUC010000004 DAOQUC010000000
VERSION     DAOQUC010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9028
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8579_length_9028_cov_3.380475"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..764
                     /locus_tag="PKH07_00130"
     CDS             <1..764
                     /locus_tag="PKH07_00130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33388.1"
                     /translation="DEQWPLIGQYIAKPNQLHHAQPGAFLHQSYWSRNWTTILPAAVA
                     LVMAIAIGAPPVILLTLVFVLLSVRAIREGYRDEWVRFVKYEGFVEAAFPVQAVNYIE
                     FYKPDPPMFSPINFGGYLIWRLSPEQYKVFTDSRFDIHGTQAAMEVLGVEDVDRRPAV
                     EAAWHRGLDEEVPEEYRDTPFWQYVLDKHGINFLITASDSRLALYLEAENRGWIEVFR
                     EKQKVLDGRFYTGYSVYVRDCPKNAALIQGARKHW"
     gene            800..1945
                     /locus_tag="PKH07_00135"
     CDS             800..1945
                     /locus_tag="PKH07_00135"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HNT33389.1"
                     /translation="MSRFHFDCRFFRNDRPCLPHKATGVRCENCVSYSPVAERILIVK
                     LAARGDVLRTTSILEPLKRRYPKGEITWLVDSVSSPLLENIPEIDRIWIKDIESLAKL
                     QVEQFDLVCNLDLSPDSCALASIPKTLERRGYILNSHGRVAPVDPVAERLMALSLRDD
                     LKRANTDTYQKMILELLNLPDPPGKIIVNLTDRERDFARQFADRIGLLADRKPVFGIN
                     VGAGGRWQNKTWKREHLLRFIQALHQRYQAPILLLGGSEEAQQISWLREHARVPLFSS
                     GTDNTLRQFMAIMSLCDAVVTGDTMALHIALGLGCKAVAFFGPTSSAEIEMYEQGLKL
                     SVPMDCLCCYQERCSKQPDCMDLLTPEEVLSAVGRLLGVQERERRDQ"
     gene            1942..2916
                     /locus_tag="PKH07_00140"
     CDS             1942..2916
                     /locus_tag="PKH07_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009144994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HNT33390.1"
                     /translation="MNKIHHDLERAQFVLRTEAAAVSAIADRLDDSFLKAIELIESCK
                     GRVVVAGVGKSGLVGRKNSATLASTGTPALFLHPAEGAHGDLGMLVNQDVVILLSNSG
                     ETREIIDLLPWMKRIGARIISMIGRANSTLGKFSDVVLDVSVEREACPHNLAPTASTA
                     AQLALGDALAIVLLQRRNFQPEDFAVLHPGGSLARQLLRVEDVMHSGAENPVVQAGAG
                     MADAIGEISSKAMGAVSVVDGKGVLLGIITDGDLRRSLSRMPDILSRKVEEVMTQNPI
                     TISVGKMAAEAIAIMENRPSQIAVLPVVDENKHAVGMVRLHDLVRAGL"
     gene            complement(3146..3529)
                     /locus_tag="PKH07_00145"
     CDS             complement(3146..3529)
                     /locus_tag="PKH07_00145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33391.1"
                     /translation="MKKVCVGFLGVLILGGLLFSQQALSNSRTEDPIGVAVAPQLLIL
                     DTVQSGEVTVHTAIPYRSVLKGSVFLNGIAASSTFADSRGQLVAKFKESLVEAIVEPP
                     SAFLTLTGLYLDGSAFSGSETIQVR"
     gene            3847..5313
                     /locus_tag="PKH07_00150"
     CDS             3847..5313
                     /locus_tag="PKH07_00150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33392.1"
                     /translation="MKSLSFLAVALVLIASPAMGETIFYLNFDEAADVIAASGTIYVP
                     GSTEIVNPAAAGLGSATIFFVDSGAPDDVNEIAADGASIGTPTGVTGHAQGGKALLTD
                     SGGCDEGLRIQVDNTMLNQSFTMEAIWFTKNVAGTGNNSGIQTIMGDNWPQLNILYEP
                     DPLRSQFQFNCRNAGQSEYYGDQSAGFSLYGESVNASNVWTHDAMVFEYNAADKSKCK
                     LSAYRNGLLVGQKIYDASTAGEEVGLFGKTVLNGNSLTIGMINSFEYRINDHRRGLNG
                     GVDAIAISNEALVPANFVLPFESPAVPEVRTGNHAVKINNNNLVWTGDMGLDIDSNRI
                     PVVAGHQVQLVFWARNGHPETSSTYSRVSYGRFSSTGFMGDTNYFANQHVPSFWVQKK
                     STVLTLAANTAFLNVAFRPGADSSIVIDDVQLIDITAGNVDLMQNGGMESWTSQGGTL
                     VPYLWRFFAVSGAVGTVEMVGDLPTPSTSIMPLLWEGF"
     gene            complement(5421..7364)
                     /locus_tag="PKH07_00155"
     CDS             complement(5421..7364)
                     /locus_tag="PKH07_00155"
                     /inference="COORDINATES: protein motif:HMM:NF027452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HNT33393.1"
                     /translation="MSSTTVSVVKDGMTSWRLFVPRSAGQVERFAADELIHYVNMISG
                     ATLQRSDRRRNTKLIIVGVRSDLDQKSLPKPKLGFDGYTISVSPETIVIAGDNPRGVL
                     YGVYDLLERLGCRWYHPQLDPKDPEVVPKSASLALSMGEWSESARIEDRYYWMSSLAF
                     TIVPERVVAQLDWAAKNRYNGVSWQCVWEKIDEHLNLMHFSGIFVQMQKRGLQLDGPG
                     HSMPYFLKTQDYFDEHPDWFGLKNAQRQPHGGPWPATNYCTSNKEANETFMANVVDFV
                     RKNPEIRQLDLIPADGGRPCECLLCQQRHATDQTVELFNELSERLEKVNPDVILTTVP
                     GYCPIELPPERSVPNGKWQGIYAHWGRNHKCSYSDADYPRRQNLLVWASYFKRFQVCS
                     YYAANSHQPFFGPPFLHALKADTEFYIEQGITGAFVLEYPFGFWWNNSFNVRMGGLYP
                     YYCPDRDPVSEIRDYAIHYYGAEAGRVLAQYFLMLGDNRNLERSYRASRGEADAGDMD
                     FFREMRTMIKRAEQLAEPNPIHSYRVSKLAMGMDFLLQLAPTRSRITAIQKVAEECIA
                     GRSDVVELRTMIADARAMIALLEAEVERLAAAENGVISEEWLKGWILKRTYSDPLLLV
                     EKKLDGLQENTPEKPNHIVDTNL"
     gene            complement(7606..>9028)
                     /locus_tag="PKH07_00160"
     CDS             complement(7606..>9028)
                     /locus_tag="PKH07_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HNT33394.1"
                     /translation="IDITDGEYLRENYQKRSWEWIDSKFFVCRERSLSLDGDRLVSRE
                     VITHVEKGVVADQFYAERLYSKESIGRLLVQAGFGEVAFPERLLTQSQRAQDLGMMER
                     RIIVTSKVRKEWTPRKQKQDEKHIVVLLGDPNKQDPLKPKGIFDDDDFYTIDQMKSAL
                     RELKGYRFTYLSNHDTLIQDLMKYAGKVDLVMNLCDEGYLNDPRKELHVPALLESLGI
                     PYTGGGPQCLAFCYDKSLVRGIAQEMGIPVPKALFVKPEDLTFELSFDFPVIVKPDLG
                     DSSFGITARSVATSAEELLNAILEIRQKFGYERPLLVEEFLTGKDISVGIIGTPPNSY
                     IVLPITEEDYSEVPDDLPKICGYEAKWCPDSPYWKIRSIPSNLPVETEKDVIKWCVEL
                     SERLECRDYVRLDWRLDEDGNPKLLEVNPNPGWCWDGHLAKMSAHMGTSYSEMLYAII
                     RAAEERLGTDNEEVRRVPRAAGE"
BASE COUNT         2079 a         2424 c         2412 g         2113 t
ORIGIN      
        1 ttgacgagca gtggccgctc atcggtcagt acatcgccaa accgaatcag ttgcaccacg
       61 cgcagcccgg agcgtttctg catcaaagct actggtcgcg aaattggacc acgattctgc
      121 cggcagccgt cgcgctggtg atggcgatcg caatcggagc tccgcccgtc attttgctga
      181 cgcttgtctt tgtgctgctc agtgttcggg cgattcggga gggatatcgc gacgagtggg
      241 taaggttcgt caagtatgaa gggtttgtcg aggcggcttt tcctgttcag gcggtgaact
      301 atatcgagtt ctacaagccc gatcctccca tgttcagccc gatcaacttc gggggatacc
      361 tgatctggcg tttgtcaccc gagcagtaca aggtctttac cgacagccgt tttgatatcc
      421 acggcaccca ggctgccatg gaagtgcttg gagtcgaaga cgtcgatcgg cgtcccgcgg
      481 tcgaggccgc ttggcatcga ggtttggacg aggaagtgcc cgaggagtat cgcgacacac
      541 ccttctggca atacgttctg gacaagcacg gcatcaactt cctcatcaca gcatccgact
      601 cccgactcgc tctgtatctc gaggccgaga acagaggttg gatcgaggtt tttcgcgaga
      661 aacagaaggt ccttgatggt cgtttctaca caggttattc ggtgtatgtt cgggactgtc
      721 cgaaaaatgc agccctgatt cagggtgcac ggaagcattg gtagaaaggc tctgccagat
      781 gaaactgtaa ggattcagaa tgagccgttt tcactttgat tgtcgttttt ttcgaaatga
      841 ccgtccgtgc cttccccaca aggcgacggg tgtgcgctgt gagaactgtg tttcgtacag
      901 ccctgttgca gaacgaatcc tcatcgtcaa actcgccgcg cgtggcgatg ttcttcgaac
      961 aacttccatt ctggagccac tcaagcgccg atatccgaaa ggtgaaatca cgtggttggt
     1021 tgattctgtg tcttcgcctc tgttggagaa catccctgag attgatcgca tctggatcaa
     1081 ggacattgaa tcccttgcca aactgcaggt tgagcagttc gacctggtct gcaatctgga
     1141 tctctctccc gacagttgtg ctctggcatc cattccaaag acacttgaac gtcgaggtta
     1201 catactcaac tcacatggaa gagtagcacc ggttgacccg gttgcagaac gactgatggc
     1261 gttgtcgctt cgcgacgatc tcaagagagc aaacacggat acctatcaga agatgatcct
     1321 cgaactgctg aatcttcctg atccgcctgg caagattatc gtcaacttga ccgatcggga
     1381 gcgagatttt gcccgccagt ttgccgaccg catcggattg ttggccgatc gtaagcccgt
     1441 ctttgggatc aatgtcggag caggcggacg ttggcagaac aagacctgga aacgcgagca
     1501 ccttttgcgg tttattcagg cactgcacca acgctatcag gcaccgattc tgctgcttgg
     1561 tggctcggaa gaagcacaac agatctcctg gctcagagag cacgctcgcg ttccactgtt
     1621 ttcgtctgga acggacaaca cgctacgcca gttcatggcc atcatgagcc tgtgcgatgc
     1681 agttgtaacc ggtgatacga tggcgttgca cattgctttg ggactcggct gcaaagccgt
     1741 tgcttttttt gggccaacgt cttcagctga gatagagatg tacgagcaag gactgaaact
     1801 ttctgtcccg atggactgcc tgtgttgcta ccaggagcgg tgcagcaagc agcccgactg
     1861 catggatcta ctgacacctg aagaagtgct ttcagcggtt ggaaggcttc tcggagttca
     1921 ggaaagggaa cgaagagacc aatgaacaaa atccatcacg atctcgaacg cgcacaattc
     1981 gtcttgagaa cagaggcggc ggcagtctct gccatcgcag accgtctcga cgactccttt
     2041 ctgaaggcca ttgaactgat cgaaagctgc aaagggcgag ttgtcgttgc cggtgtcgga
     2101 aagtccggtt tggtcggacg gaaaaattcc gcgacacttg ccagcacggg cacccctgcg
     2161 ctgtttctgc atccggcgga aggggcgcat ggcgacttgg gtatgttggt caatcaggac
     2221 gttgtcatcc tcctgtcaaa tagcggtgag actcgggaga tcattgatct tctgccttgg
     2281 atgaaacgca taggcgctag gatcatctca atgatcggca gggcgaattc cacccttggc
     2341 aagttcagcg acgtggttct ggacgtttcg gtcgagcgcg aagcctgtcc ccacaacctg
     2401 gctcccacgg caagcaccgc agcgcaactg gcacttgggg acgctctggc gattgtgctg
     2461 ttgcagcgac ggaatttcca gcctgaagac tttgccgtgc ttcaccctgg tggatcgttg
     2521 gcaaggcaac tgcttcgagt tgaagatgtg atgcactcgg gagcagagaa tcccgttgtt
     2581 caagccggtg ccggcatggc agatgccatt ggggaaatca gttcaaaggc catgggcgct
     2641 gtcagcgtgg tggatggcaa gggagttctc ttggggatca tcacggacgg tgacttacgc
     2701 agatctctct ccaggatgcc ggacatcctc tccagaaaag tggaagaggt catgactcaa
     2761 aacccgatca ccatctcagt tggtaagatg gccgcagaag ccattgcgat catggagaat
     2821 cgtcccagcc agattgctgt gctgcctgtc gtggatgaaa acaaacacgc cgttggaatg
     2881 gttcgtctgc acgatctcgt ccgcgccgga ctgtagggtt cctccggaca ggccagcgct
     2941 cgcgcgcgcg tgggctatgc ccactctctc ggacgcttac tcaatagatg ccacactttt
     3001 ctcgactctg tgtcacgaag aactatttcc gtggcttcgg caaaacaaga agaagcagcc
     3061 cacgtttgtc gaaggctgct tcgaggagaa tacggatact ctgtacctgg cagacggcca
     3121 gggagcgcat cagtctcgtt cgtgtctatc gcacttgtat tgtttctgag ccagagaaag
     3181 cactcccgtc gagataaagg cctgtgagcg tcaaaaacgc ggaaggcggt tcgacgatcg
     3241 cttccaccaa ggattcctta aacttggcaa ccaactgtcc acgtgagtcg gcaaaagtgg
     3301 agctggccgc gatgccattg aggaaaacgc tgcccttaag cacgctccga taggggatgg
     3361 ccgtgtgaac agtcacttcg ccgctctgaa ccgtgtccag aattagcagt tgcggtgcca
     3421 cggcgacccc gatgggatct tccgtccggc tgttcgagag tgcctgctgc gaaaacagca
     3481 atccgccgag aatcagaacg ccaagaaaac ctacgcatac cttcttcatc ttaccctcct
     3541 gtgtcatgcc atgtaatcct ctgcctcgtg aagcgtcaag aggctccgac aaagattgtc
     3601 acactctact taaccttaac gtcatttcac aagaggcgtg ttcagtattt cagagaaata
     3661 tcgcacgaaa tgcaaactgc ctcatgagac ttccgactga agacccgtgg gaacatacgt
     3721 tgacacgaca gggtggacat tggtcttttc tgttgacatg caatgcttca gaagagtagt
     3781 ttttgtctat ctagaaggct gttccatcca gcagggcaat cggatttgat gaaaggagta
     3841 tcaggaatga aatcattgtc ttttttggca gtcgctttgg tgttgatcgc ctccccggca
     3901 atgggagaga caatcttcta tctgaacttt gacgaggccg cggatgtgat cgcggcaagc
     3961 ggaacgattt atgttccagg cagtacggag atcgtgaatc cggcggcggc aggacttggt
     4021 tcggcaacaa tcttctttgt tgacagcggc gcccccgacg atgtcaatga gatcgctgca
     4081 gatggtgcat ccattggaac gccgaccggt gttaccgggc atgcgcaagg tggaaaagct
     4141 cttttgacag actccggtgg ttgtgacgag ggtcttcgaa ttcaggtgga caacacgatg
     4201 ctgaaccaga gtttcaccat ggaagccatc tggttcacca agaacgtggc tggcaccggg
     4261 aacaattcgg gtatccagac gatcatggga gataactggc ctcagttaaa catactgtat
     4321 gagccagatc cacttcggag tcagttccaa ttcaactgtc gaaatgcggg tcagagtgag
     4381 tactatggtg atcagtctgc aggcttttcc ctttacggcg agagtgtgaa cgccagcaat
     4441 gtctggactc acgatgcgat ggtgttcgag tacaacgcgg cggacaagtc caagtgcaag
     4501 ctcagcgcgt accgcaatgg actcctggtg ggtcagaaga tttatgacgc atcgacagcg
     4561 ggtgaagaag taggtctttt cggaaagact gtcctcaacg gaaacagcct gactatcggc
     4621 atgatcaact cattcgagta ccgcatcaac gatcaccgcc gaggcctgaa tggcggcgtt
     4681 gatgcaatcg ccatctccaa cgaagcattg gttcccgcca acttcgttct gccttttgaa
     4741 tcacccgcgg ttccagaagt gcgcacagga aaccacgcag tcaagatcaa caacaacaat
     4801 ctggtctgga cgggcgacat ggggctcgac attgacagca atcgaattcc cgtcgtcgca
     4861 ggccatcaag tgcagttggt attctgggcg cgcaacggcc atccggaaac aagcagtacc
     4921 tacagccgcg tttcctacgg ccgcttctca agcacgggct ttatgggtga caccaactac
     4981 ttcgccaacc agcatgtacc ttccttctgg gtacagaaga aatcaaccgt cctgacgctt
     5041 gccgcaaaca cggcgttctt gaatgtagcg ttccggcccg gggcagacag ttccatcgtc
     5101 attgatgatg tgcaactcat tgatatcacc gccggcaacg ttgatttgat gcagaatggc
     5161 ggaatggagt cctggacaag tcagggtgga actctcgttc cttatctctg gcgtttcttc
     5221 gccgtgagtg gcgctgttgg aacagtcgag atggtgggtg atctgcccac gccctcgacg
     5281 agtatcatgc ctctcctttg ggaaggcttc tgagcaggtc tgcacccgtc gcggacaggt
     5341 gggctgtgcc cactcgtgtg tgagttaaaa gcaaccaccc tcttcccgag ggtggttgct
     5401 ttttttcctg cagcgagaga ctagaggttc gtgtctacga tatgattggg cttctcgggt
     5461 gtgttctcct ggagtccgtc gagtttcttt tctaccaaca gcagaggatc agaataggtt
     5521 cgcttgagaa tccacccttt gagccactcc tcagagatca caccgttctc agccgcggcc
     5581 agacgctcca cttccgcttc gagcagggcg atcatcgcgc gcgcgtcggc gatcatcgtg
     5641 cgcaattcca caacgtcact acgacctgca atacattcct cagccacttt ctgtatagca
     5701 gtgattcgag agcgagtcgg tgccaactgc agcaggaaat ccatccccat cgccagtttt
     5761 gaaacacggt aggaatggat cggattcggt tccgccaact gttctgcccg cttgatcatc
     5821 gtccgcatct ctcgaaagaa gtccatgtcg cccgcgtccg cttctccgcg ggaagcacga
     5881 tacgaacgtt ccagatttcg attgtcgccg agcatcagga aatactgcgc aagaacacgt
     5941 ccggcctcag caccgtagta atgaatggcg tagtctcgaa tttctgagac tggatctcga
     6001 tcgggacaat agtagggata cagtccgccc attcggacgt tgaaggagtt gttccaccag
     6061 aatccgaaag gatactccag aacaaatgca cctgtgatcc cctgctcgat atagaactcc
     6121 gtgtctgctt tcagcgcatg cagaaacgga ggcccgaaaa acggctgatg ggaattggcc
     6181 gcgtagtagg aacagacttg aaaccgcttg aagtaggacg cccacaccag aagattctgc
     6241 cgccgcggat agtctgcgtc gctgtaactg catttgtggt tccgacccca gtgcgcatag
     6301 atgccctgcc acttgccatt tggaacagaa cgttcagggg gcagctcgat aggacagtaa
     6361 ccaggcaccg tcgtcaggat gacatctgga ttgaccttct ccagtctttc ggacagctcg
     6421 ttgaacagtt ctaccgtctg atcggttgca tgacgttgct gacacagcag gcactcacag
     6481 ggacgtcccc catcggctgg aatcagatcc aactgcctga tctcagggtt ctttctgacg
     6541 aaatccacga cattggccat gaaggtctca ttggcttctt tgttgcttgt gcagtagttg
     6601 gttgcaggcc atggaccgcc atgcggttgc ctctgagcat tcttcaaacc aaaccagtcg
     6661 gggtgctcat caaaatagtc ctgtgttttc aggaaatacg gcatggagtg cccagggccg
     6721 tccagttgca gcccccgctt ctgcatctgt acgaagatgc cgctgaagtg catcagattc
     6781 agatgctcgt ctatcttctc ccagacacac tgccagctga ctccgttgta acggttcttg
     6841 gcagcccagt ccaactgagc aacgactcgt tcgggaacga tggtgaacgc cagactgctc
     6901 atccagtagt agcgatcttc gatccgagcg gactcagacc actctcccat ggagagagcg
     6961 agactggcgc tcttgggaac gacctcagga tctttgggat caagctgtgg gtgataccaa
     7021 cgacacccaa gccgttcgag cagatcatag acaccataga gcacaccacg aggattgtcc
     7081 cccgcaatga cgatggtttc gggagagacg gaaatcgtgt aaccgtcaaa tccaagtttc
     7141 ggcttcggaa ggctcttctg atccaaatcg gaacgaacac cgacaatgat gagctttgtg
     7201 tttctgcgtc tgtcagatct ttggagagtc gctccagaga tcatgttgac atagtggatg
     7261 agttcatccg ccgcaaaacg ttcgacttgt ccggcagacc gtggaacaaa gagtctccag
     7321 ctggtcattc catccttgac aacggatacg gttgttgatg acatgcaagt tgcctccagt
     7381 cgttgtgtca agtcctggtt cagcgcattg tatggatgtc tctatctgaa ttcccagatt
     7441 cgaaatccaa agtcacactc aaagagactg cggctcacaa accttgcatg aatgccgcac
     7501 gacacagcat cattgagtag aggccacccc gtggtgcata cgatctcatc gtgctcatcc
     7561 agaactcaaa gcacatacga gacatgttac actggacaga gccgtctact ccccagcggc
     7621 tcttggaact cgacgcacct cttcattgtc tgtgccaagc cgttcttcag ctgcccgaat
     7681 gatggcatag agcatttccg aataggacgt gcccatgtgt gccgacatct tggcaagatg
     7741 cccgtcccag caccaacccg gattcggatt gacctcaagg agtttgggat tgccgtcttc
     7801 atccaaacgc cagtcgagtc gaacgtagtc ccgacattcc agacgctcgg aaagctccac
     7861 gcaccacttg atgacatcct tttccgtttc aacaggaaga ttgcttggaa tagaacgaat
     7921 cttccagtaa ggggaatcgg gacaccattt tgcctcatat ccgcaaatct tgggaagatc
     7981 atcagggact tcggagtagt cttcctcggt aatcggcagc acaatatatg agtttggtgg
     8041 tgttccaatg ataccgacgc tgatgtcttt gcccgtcagg aattcctcaa ccagcaacgg
     8101 ccgctcgtat ccaaacttct ggcggatttc cagaatcgcg ttgagcagtt cttccgcgga
     8161 tgttgccaca ctgcgggcag tgatgccaaa gctggaatca cccaagtccg gcttgacaat
     8221 caccggaaaa tcgaaggaca actcaaaagt caggtcctct ggtttgacaa ataaagcttt
     8281 tggtacagga attcccatct cctgggcaat tcctcgaacc agagacttgt cgtaacagaa
     8341 cgccaggcat tggggtccac ctcccgtgta cggaattccg agggactcca agagcgccgg
     8401 cacgtgcagt tccttgcgcg gatcattgag atagccttcg tcgcagaggt tcatcaccaa
     8461 atccaccttc ccggcatatt tcatcaagtc ctggatcagg gtgtcgtgat tgctgagata
     8521 ggtgaaacgg taacccttca gttcccgaag cgccgatttc atctggtcaa tggtgtaaaa
     8581 atcgtcgtcg tcgaaaatcc ccttgggctt cagtggatcc tgtttgttgg gatctccgag
     8641 cagcacgaca atatgctttt cgtcttgttt ctgtttgcgg ggcgtccatt cttttcggac
     8701 cttcgatgtg acgatgatgc gtcgttccat catacccaaa tcctgggcac gctgggactg
     8761 tgtgagcagt ctttccggaa aggccacctc gccaaaaccc gcctgaacca agagcctgcc
     8821 aatgctctcc tttgaataga gacgttcggc atagaactga tccgccacca cgcctttctc
     8881 cacatgggtg atgacttcac gagagaccag ccggtcgccg tccagtgaaa gagaccgttc
     8941 gcggcagacg aagaacttgc tgtcaatcca ttcccatgag cgcttttggt aattctcccg
     9001 aagatattcc ccatcggtga tgtctatg
//
LOCUS       DAOQUC010000005         8670 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_9012_length_8670_cov_3.337203, whole genome shotgun sequence.
ACCESSION   DAOQUC010000005 DAOQUC010000000
VERSION     DAOQUC010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8670
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9012_length_8670_cov_3.337203"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2868)
                     /locus_tag="PKH07_00165"
     CDS             complement(<1..2868)
                     /locus_tag="PKH07_00165"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNT33395.1"
                     /translation="MYKSRFTVTLIVIGAMVLLGGSLSVQAADWPMWRCDAGRRAESP
                     EDLPAELSLQWTHVYTSREMVWDDPLNQDLMPYDRVFEPVVIGKMLFIGFNDSDKVVA
                     LNTETGREQWRFYVDAPVRLPVAAWKDRIFFTSDDGCLYCLSAKNGQMLWRFQGAPDK
                     RRILGNKRLISTWPARGGAVVEDGVVYFAASIWPFMGTFIYALDAMTGKVVWENSGNG
                     STYMLQPHQSPAFAGVAPQGVLVIANKRVLVPGGRSVAACFEKETGKYLYYRLSDNGK
                     SGGSFVCAIGDLLFNHHREKQVSLFDAEKGDSLIGMIGKQPVLTSNVWYFMGEKIRAF
                     DPAKTLADRKKWREGMLWELDVNASGDLIKAGSRLYAAGDGRITAIDVRELSSPKVVW
                     SKVVEGTVERLIAADGKLFAVTLDGRILAFGHSKKPSQAVTIQEPLAAVEPTLESNER
                     ARHLLDQTGVRAGYCLFYSPGNEEILAALLSNSDLHIVAVEPDAAKVGMLRKKLDALQ
                     LYGKRAAVIQGEPTTFDAPPYLSSMTILGEGVALDVQTLQRLYQSLRPYGGTLVSLGL
                     TSERFAALAGQAQLENAEVSSDGVFLVRKGALKGAGQWTNQYGSMDNSAKSDDALVAL
                     PLGVLWFGGSSNLDVLPRHGHGPPEKVVGGRLFIQGLDNLSARDVYTGRVLWKTPLQD
                     LDTYNVYFDGSYKDTPTSTRYNQEHIPGANIRGTNFVATADRVYVVQGSQCDVLNAES
                     GQLEESILLPPIDPKAEVPKRRQWAFIGVEGDTLFGGNGFVSFSDHTKLAKDEYSIWT
                     DFDSGASRELIAFDRLSGSIRWRFDDVKHGLLHNAIAAGNGKVFVLDKLPPHIEKFLG
                     RRGQQIPSNYSLTALSLDTGNILWKQSAPALPSGTGDKQKGGDIFGSFLCYSVQHDVL
                     IQSTRPSRDMVKDEEGKRMMALRGSDGTLIWDTTHEYATFPI"
     gene            complement(2900..4135)
                     /gene="rodA"
                     /locus_tag="PKH07_00170"
     CDS             complement(2900..4135)
                     /gene="rodA"
                     /locus_tag="PKH07_00170"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HNT33396.1"
                     /translation="MQDGRSIERFDFYLFCLLCALLGMSLLIIFGATSSSGVMRSKAY
                     TQLAAVVIGIALVIMMQLVDYRTILDNAYTVYWSCVGLLLGVLVFGLVAKGSKSWFVV
                     PVIGFRFQPSELMKVGLIIGLARCLSKRDRSLDGLKEMLIPLILIGIPFLLVLAQHDA
                     GTALVFPLVAMAMFWAAGVRKIYLFLLLSPALGIMGIRGGFVSLFIFAVLLFILFRIA
                     IKVGVPWLDWCIFLAVNVVSYFAAPMAWEFLKPHQRQRLMVFLEPNVDPMGISWNLIQ
                     SKVALGSGGFWGKGWQQGTQIRGQFLPAFHTDFAFSVLGEEWGFVGCLILMVIFFLFL
                     IHGLEIAQSTRYLPGALVVVGVVALFFIHILVNVGMCMGLMPIIGIPLCFISYGGSAL
                     VANALAASFLFNVQIHRFK"
     gene            complement(4104..5951)
                     /gene="mrdA"
                     /locus_tag="PKH07_00175"
     CDS             complement(4104..5951)
                     /gene="mrdA"
                     /locus_tag="PKH07_00175"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HNT33397.1"
                     /translation="MLTTMPKNVERSLGDFPKQFLILITVCFLVLILRLWALQVLEAD
                     YYRQRAERNYMREVSVPALRGRVFDAQGTLMADNLPSYEMIMNCRAISSSAKERNIEM
                     LSGILGLPQEMLSERVQTRPADRFGDKILLRDMEFADVVAVAENLPSLSGVQIRSRPK
                     RIYPLGHNACHVLGYIGEVSQRELTIHKEEEDYQPGDDIGKMGLERQYEKYLRGSKGI
                     RRVQVSSKGLWEEDLREIKPNLPGGDLHLTLDAELQAFTDKLIESTPGAIVLLDPRNG
                     GILAMSSSPTFDPNFFCGPIDPEAWRTLQSDETHPLQNRAIASMYPPGSVFKLILAWA
                     GLNERIVNNQTSFSCTGVFHLGSWPFRCHQRYGHGSMNVRDAIRYSCDVYFYELGLKL
                     GIERIGDYSYRLGCGTTLGIDLPQERAGLIPDPKWKKQVKSEPWYPGDTVNSSIGQGF
                     VMTTPLQIACCYAVLANGGTLYRPHVLRRVVDYMGRTVLENSSGEIIHKVAISNADWR
                     TLMDAFSSVVNDNGTGRRTKLPHVEICGKTGTAQAGTTREPHAWFVGFAPRENPSVVA
                     VVFVEHGGHGGEQAAPIVKDLFLKLFPKPGKQSLTEVTEDAGRSEHRAL"
     gene            complement(5962..6492)
                     /gene="mreD"
                     /locus_tag="PKH07_00180"
     CDS             complement(5962..6492)
                     /gene="mreD"
                     /locus_tag="PKH07_00180"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HNT33398.1"
                     /translation="MRIWRILLFAVIVLFLQVWIGPAFRNLGAEPNFVLLLVIFVSCS
                     HDPLHGMLLGFTLGMAEDVLRMQCFGLSALTYVLVGYLPRVLGNLLFLENRVAQFIYL
                     ILAHLLKIVLEGTVESALTHSLPEHNVFLLQRLLSLVLDAILLPLLFFVWFRTIAAKE
                     NPTQQTRTWRSVAPRR"
     gene            complement(6544..7458)
                     /gene="mreC"
                     /locus_tag="PKH07_00185"
     CDS             complement(6544..7458)
                     /gene="mreC"
                     /locus_tag="PKH07_00185"
                     /inference="COORDINATES: protein motif:HMM:NF016011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005618 - cell wall [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HNT33399.1"
                     /translation="MKLSYRSSSSVVRQRRTGFHREVVLAILCLLCFISIARWGRDAS
                     EGYPRLLGLLLEPFRITVSSLTAIEGEIQDFFGSFLELRTQTEAAKDEIEHLKQELIA
                     ANQREEQLRTLLPLAQVFEPTQFDTVRATVVGQGGTDYRTLILDQGSNNGVGPNMPVF
                     VGSGLVGRLDRVLPNASRVVLVQDRNSAVGAEVRSETGEVLVEGLVYGTGSSLDDTLR
                     LEVAGVTTPLEGAWVYTSSLSVYYPSGLQIGRVERTVPGIAQVFERYVVRPAVDVRSV
                     RQVFILTNLNRGDSLTLITEEGASERLR"
     gene            complement(7692..>8670)
                     /locus_tag="PKH07_00190"
     CDS             complement(7692..>8670)
                     /locus_tag="PKH07_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012524198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HNT33400.1"
                     /translation="ANTLVYVKGQGIVLCEPSVVAIVKGSNEVKAVGSEAKKMLGRTP
                     GNIVAIRPMKDGVIADFDVTEAMLRYFIRKVHNRTRMFRPTIIVGVPSGITSVEKRAV
                     RDSALHAGAVDVFLIEEPMAAAIGAGMPVAEPSGSMVVDIGGGTTEVAVISLGGIVVS
                     NSIRIAGDELDEAIIAHVKRKYNLLIGERTAEEIKIRIGSAYPMDPECTMQIKGRDLL
                     TGLPNTIEVTSEEVRDAIAEPVNAIVDTVKATLEKTPPELAADIMERGLVLAGGGALL
                     RGLDTLINRETGLPVCLADDPLTAVVLGTGKALDEIETLRDVLQGCEDF"
BASE COUNT         2171 a         2529 c         2157 g         1813 t
ORIGIN      
        1 gattggaaac gtggcgtatt cgtgcgtcgt gtcccagatc agcgtgccgt ccgatccccg
       61 aagcgccatc atccgcttgc cctcttcatc tttgaccata tcccgtgatg gccgtgttga
      121 ctgaatgaga acgtcatgct gaacagagta acaaaggaag gaaccgaaga tgtcaccgcc
      181 cttctgcttg tcccctgttc cgctgggcag cgcaggagcg ctctgcttcc aaaggatatt
      241 gcctgtgtcc agactcaagg ccgtcagact gtaatttgag ggtatctgct gcccgcgtct
      301 gccgagaaac ttctcaatgt gcggtggcag tttgtccagg acaaaaacct tgccattgcc
      361 cgcagcgatg gcgttgtgca gcaagccgtg tttgacgtca tcaaagcgcc agcgaatcga
      421 accggacagc cgatcgaatg cgatgagttc tctgctcgct cccgaatcga aatcagtcca
      481 gatggagtac tcgtccttgg caagcttggt gtgatcagag aatgagacaa atccatttcc
      541 gccgaacagt gtatccccct caacaccgat aaatgcccac tgtcggcgct ttggcacttc
      601 tgccttggga tcaatgggtg gaagaagaat gctctcttcg agttggccac tttccgcatt
      661 gagcacatca cattgacttc cctgaacaac gtagacccga tcggccgtag cgacgaaatt
      721 ggtgcctcgg atattggctc cgggaatgtg ttcctggttg tagcgtgtgc ttgttggcgt
      781 gtctttgtag gaaccatcaa agtagacgtt gtaggtgtcc aaatcctgaa gcggggtttt
      841 ccacagcact cgacctgtat agacatccct tgcactcaga ttgtcgagac cttggatgaa
      901 cagccttccc ccaaccactt tttcaggcgg cccatgtcca tgtcgcggaa gcacatccag
      961 attggaactg cccccaaacc agagaacccc cagcggaagc gcaaccaagg catcgtcaga
     1021 cttggcggaa ttgtccatgc tgccgtattg gtttgtccat tgacctgcgc ctttcagagc
     1081 acccttgcgc acaagaaaga cgccgtcgga agaaacctca gcgttctcca gttgcgcctg
     1141 cccggcaagt gccgcaaaac gctctgaagt cagtccaaga gaaacaagcg ttccaccata
     1201 gggacgaagc gactggtaaa gccgctgcaa cgtctgaacg tccagtgcaa caccttcacc
     1261 cagaatcgtc atcgaagaaa gatacggcgg tgcatcgaaa gtcgtcggtt ctccctgaat
     1321 gaccgcggca cgcttgccat agagttgaag cgcatcgagt ttctttctta acatgccaac
     1381 cttcgcagca tcaggttcaa cggcgacaat atgaaggtcg gagttgctca aaagtgccgc
     1441 aagaatctct tcattgcctg gactgtagaa caggcagtaa ccggcacgca caccggtttg
     1501 atcaagcagg tgtcttgcac gctcattgct ttcgagtgtc ggttcgacag cagcaagcgg
     1561 ttcctgaatg gtcacggcct gagagggttt cttggagtgg ccaaaagcga gaattcgacc
     1621 gtccagcgtc acggcaaaca gttttccatc agccgcgatc agtcgttcaa cagttccctc
     1681 aaccaccttc gaccagacca ccttgggtga agacaattct ctgacatcaa tcgcggtgat
     1741 tcttccatca cccgcagcat agagacgact tcccgctttg atcagatcgc ccgaggcatt
     1801 gacatccaac tcccacagca ttccctcacg ccatttcttg cggtcggcga gagtctttgc
     1861 aggatcgaat gctcttatct tctctcccat gaaataccag acattgcttg tcagaacagg
     1921 ctgcttgcca atcatcccga tcagggagtc gcctttctca gcatcaaaga gcgagacctg
     1981 tttctctcgg tgatggttga agagaagatc tccaatcgcg cagacaaagg aaccaccgga
     2041 cttcccattg tcggaaagac ggtagtagag atacttgccg gtctccttct caaagcaggc
     2101 tgccacagag cggcctcctg gaacgagaac ccttttgttg gcaatcacca acactccctg
     2161 tggtgcaaca ccggcaaagg ctggcgactg atgcggctgc agcatatacg tcgaaccgtt
     2221 gccggagttc tcccagacaa cctttcctgt catggcgtca agtgcataga tgaacgtccc
     2281 catgaacggc cagatgcttg ctgcaaagta gacaactccg tcttcgacaa ccgcaccacc
     2341 acgcgccggc caggttgaaa tcagtcgttt gttgcccagg atgcgccgct tatccggagc
     2401 tccttgaaat ctccacaaca tctgtccatt cttcgccgaa aggcagtaca gacagccatc
     2461 atcactcgtg aaaaaaatcc tgtccttcca ggcagcaacg ggtaaccgaa cgggagcatc
     2521 cacatagaag cgccattgtt ctctgcccgt ctccgtgttc agcgcaacga ccttgtcgct
     2581 gtcattgaat ccgatgaaaa gcatcttgcc aataacaacc ggctcaaaga ctcgatcgta
     2641 gggcatcaaa tcctgattga gtggatcatc ccaaaccatc tcacgagacg tgtagacatg
     2701 tgtccactga aggctcagtt cggctggcag gtcttcgggt gactctgccc ttcttcctgc
     2761 atcgcagcgc cacatcggcc agtcggccgc ttgaaccgac agagatccac caagaagaac
     2821 catggccccg atgacaatga gtgtcactgt gaaacgtgac ttgtacaaaa caattcttcc
     2881 ctccatctag actggcagat cacttgaagc gatgaatctg aacgttgaac agaaaagagg
     2941 ccgcaagcgc gttggcaacc aaagccgacc ccccgtatga gataaaacac aagggaattc
     3001 cgatgatggg catcaacccc atgcacatcc ccacattaac aagaatgtga atgaagaaca
     3061 gcgccacaac acccacaacc acgagcgctc caggcaagta tcgcgtggac tgggcaatct
     3121 ccagcccatg gattaagaac agaaaaaaga tcaccatgag gataagacat ccaacaaaac
     3181 cccattcctc tcccaaaacc gagaaggcaa agtccgtgtg aaacgcaggc aggaattgtc
     3241 cccgaatctg agtgccttgc tgccagcctt tgccccagaa accgccggag ccgagcgcga
     3301 ctttcgactg gatcagattc caactgattc ccatgggatc cacattcggt tcgagaaaca
     3361 ccataagcct ctggcgttga tggggtttca ggaattccca cgccatcggc gcagcgaaat
     3421 aggagactac attgacggcc agaaagatgc accagtccaa ccacggcaca cccactttga
     3481 ttgcaatccg aaacaggatg aacagcagga cggcgaagat aaaaagagag acaaatcccc
     3541 ctcgaatacc cataattccc aacgctggtg aaagcagcaa gaacagatag atctttcgca
     3601 cacccgccgc ccagaacatt gccatcgcaa ccaggggaaa gaccagtgcc gttccagcgt
     3661 catgctgtgc caataccaga agaaaaggaa taccgatcag gatcagcggg atgagcatct
     3721 ctttgagtcc atcgagagac cgatcccgct tggaaagaca tcgcgccaaa ccaatgatga
     3781 gtcccacttt catcagctcg gacggctgga atcgaaagcc aatgactggc accacaaacc
     3841 aactcttgga acctttggca acaagaccaa aaaccaggac tcccaagagc aatcccacac
     3901 atgaccaata gacggtgtag gcattgtcca gaatcgtccg gtagtctacc aactgcatca
     3961 ttatcaccag cgcaattccg atgacaacgg cggccagctg ggtgtatgcc ttggaacgca
     4021 tgactcctga cgaactggtg gcaccgaaga tgatcagaag cgacatcccc agcaatgcgc
     4081 acaagaggca gaaaagatag aagtcaaagc gctcgatgct ccgaccgtcc tgcatcttcg
     4141 gtcacctccg taagactttg cttccctggt ttgggaaaga gtttcaggaa cagatccttg
     4201 acaatcggag cagcctgttc tccaccgtgg ccgccgtgct ccacgaagac aacggcaaca
     4261 acgctggggt tctcccgagg tgcaaaacca acaaaccacg cgtggggttc ccgtgttgtt
     4321 cccgcttggg cggttcccgt ctttccgcag atctccacat ggggaagttt tgtgcggcga
     4381 ccggttccgt tgtcattgac gacagaggaa aaagcatcca tcagcgttcg ccagtctgca
     4441 ttcgagatgg cgactttatg gatgatctca cccgaggaat tctccagtac agtccgaccc
     4501 atgtagtcaa ccactcttcg aagcacatgg ggtcggtaga gcgtgccgcc gttcgccagc
     4561 actgcatagc agcaggcaat ctgcagggga gtcgtcataa caaagccttg gccgatggat
     4621 gaattcactg tatctccggg ataccacggc tcggacttga cctgcttctt ccatttcgga
     4681 tcgggaatca gaccagcacg ttcctgcgga agatcaattc ccagagttgt gccgcaaccc
     4741 agccgatagg agtagtcgcc gattcgctca atacccagtt tcagccccag ttcatagaaa
     4801 tagacatcgc aagagtatcg aatagcgtca cgaacattca tactgccgtg tccgtacctc
     4861 tggtgacatc ggaatggcca actccccaga tgaaaaacac ccgtgcagga gaaactggtt
     4921 tggttgttga cgatgcgctc attgagtccc gcccatgcaa gtatcagttt gaagacagaa
     4981 cccggtggat acatgctggc aatcgcccga ttctggagag gatgcgtctc atcggactga
     5041 agtgtgcgcc aggcttcggg atcgattggg ccgcagaaga aattcggatc aaaggtcgga
     5101 gaagaggaca ttgcgagaat gcctccgttg cgcggatcca gaaggacaat tgccccaggt
     5161 gtggattcaa tgagcttgtc agtaaacgcc tgaagctccg catccaatgt caggtgcaga
     5221 tcgcctccag gaagattggg tttgatctcc cgcagatctt cttcccagag cccctttgag
     5281 gacacctgaa ctctgcgaat ccctttggag cccctgaggt acttctcgta ttgtctctcg
     5341 agccccatct ttccgatgtc atcacccggc tgatagtctt cttcttcttt gtggattgta
     5401 agttcccgtt ggctgacttc accgatgtag cccagaacat gacaggcgtt gtgaccgagg
     5461 gggtagattc gtttcggacg tgaacgaatc tgaacccctg acagagaggg aagattctca
     5521 gccaccgcca ccacgtccgc aaactccata tcccgaagca gtattttgtc accaaatcgg
     5581 tcagccggcc ttgtctggac acgctcggaa agcatctcct gtggaagccc taaaatgccc
     5641 gaaagcattt cgatgtttct ctctttagca gaagaggaaa tcgccctgca gttcatgatc
     5701 atttcgtagg acggcagatt atccgccatg agagtgcctt gggcatcaaa gacgcgacca
     5761 cgaagtgcgg ggacagagac ctcacgcatg taattgcgtt cggctcgctg ccgatagtag
     5821 tccgcttcca gaacctgaag tgcccacaag cgcaggatca gcaccagaaa gcagaccgtg
     5881 atcaagatca ggaactgctt gggaaagtct cccagggaac gttccacatt tttcggcatt
     5941 gtggtcaaca taacacttct ctcagcgtct gggcgctacc gatctccacg ttcgggtctg
     6001 ctgcgtcgga ttctccttgg ccgcaattgt cctaaaccac acaaagaaaa gcaatggaag
     6061 caggatcgcg tccagtacaa gcgacagcag tcgttgcagc aggaacacgt tgtgctcagg
     6121 caggctgtgc gtcagagcag attcaaccgt accctccaaa acgatcttca gcagatgcgc
     6181 aaggatgaga tagataaact gcgcgacgcg attctccaga aatagaagat ttccaagaac
     6241 tcgcgggaga tatcccacaa gcacatacgt caaggcactc aggccaaaac actgcattcg
     6301 gagaacatct tctgccatcc cgagcgtgaa tcccaaaagc atcccgtgca gaggatcgtg
     6361 actacaactg acgaaaatca ccagcagcag aacaaaattg ggctccgccc ccaggtttcg
     6421 gaacgcaggc ccaatccaaa cctggagaaa caggacgatc acagcgaaca gcaaaattct
     6481 ccaaatacgc atcaaacact ccaggcagac aacccgtcgg ctgcaagcct ctttctctct
     6541 ccgtcagcgc agccgttcgg acgcgccttc ttccgtgatc agagtcagtg aatcacctcg
     6601 gttgaggttg gtcaagatga agacctgacg aaccgatcga acatctaccg caggtcgcac
     6661 aacgtagcgc tcaaacactt gggcaatgcc gggcacggtt cgctcaactc gaccgatctg
     6721 cagcccggat ggatagtaga cactgagcga tgaggtgtaa acccaggcac cttccagagg
     6781 cgtggtgaca ccagccactt ccaaacgcag ggtgtcgtcc agagagctgc cggtaccgta
     6841 gacaagtcct tccaccaaga cttctccggt ctcagagcga acctctgcac cgacggcgct
     6901 gttgcgatct tgaacaagca caacacgact ggcattggga agaacccggt cgagtctacc
     6961 caccagcccg ctgcccacaa aaacgggcat attcggaccg acaccgttgt tggagccttg
     7021 gtcgaggatg agcgttctgt agtccgtgcc tccctgaccc acgacggtgg cacgcactgt
     7081 gtcaaactgc gttggttcaa aaacctgcgc cagaggaaga agcgttctga gctgttcctc
     7141 cctctgattg gcagcaatga gttcctgttt gaggtgttcg atctcatctt tagccgcttc
     7201 ggtctgcgtc cgcagttcca ggaaactgcc aaagaaatcc tggatctcac cttcgatggc
     7261 agtcaaactc gaaaccgtga tacgaaaagg ttccagcagc agccccagca gtctggggta
     7321 gccctcgctg gcatcccgtc cccatcgggc tatggaaatg aaacagagca gacacagtat
     7381 ggccagcaca acttcccggt gaaaaccggt tcggcgttgg cgaaccacgg aagatgacga
     7441 gcggtaacta agtttcacag agcctgttcc atccagaaaa atgaagcatc caaccacttc
     7501 agtctgaagt cagggatact gagtcagaag ccactgtcct ctgagaggcg gactttcgag
     7561 aagacggaca cgccccagcc cgataccagc accaaccgat ccaagagaac tgtcgctaag
     7621 tcatgatgcc ggtgctgctc ccgcaggcgc gtgagcatga tgcgcctgca acacctgatc
     7681 atgccctgcg actagaaatc ctcgcatccc tgaaggacat ctcgcaatgt ttcgatttcg
     7741 tccagcgcct tgcccgttcc caacaccacg gcagtgagag ggtcatcggc aagacacacc
     7801 ggaagtcccg tctcccggtt gatcagagtg tccaaacctc gaagcagcgc acctcctcct
     7861 gccagtacca aaccacgctc catgatgtcc gccgccaact cagggggcgt cttttccagc
     7921 gtcgctttca cggtgtcaac gatggcgttg actggttccg cgatggcgtc tcgaacttcc
     7981 tcagaggtaa cctcaatcgt gtttgggaga cccgtcaaaa ggtctcttcc cttgatttgc
     8041 atcgtgcatt ccggatccat cggatacgcg gagccgatcc gtatcttgat ctcttctgcg
     8101 gttcgttctc caatcagcag attgtatttg cgcttgacgt gggcgatgat tgcctcatcc
     8161 agttcatctc ccgcgatgcg gatggaattg ctcacgacaa tgccacccag ggagatgaca
     8221 gccacttcag tggttccacc accgatatca acaaccatgc tgccggaagg ctcggcgacc
     8281 ggcatccctg ctccaatggc agccgccatg ggttcctcga tcagaaacac atccaccgcc
     8341 ccggcgtgaa gagcggaatc ccgaaccgca cgtttttcca cgctggtgat tccagatggc
     8401 actccaacga tgatcgtcgg tcggaacatt cgggttcggt tgtgaacctt tcggatgaag
     8461 taccgaagca ttgcttcggt cacatcaaaa tccgcgatga ctccgtcctt catcggtcgg
     8521 atggcaacga tgttgcctgg agttcgaccg agcatcttct tggcctcact ccccacggct
     8581 ttcacttcgt tgctcccctt cacgatggca accaccgaag gctcgcaaag cacgatcccc
     8641 tgccccttca cgtagacaag cgtgttggca
//
LOCUS       DAOQUC010000006         8393 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_9403_length_8393_cov_3.391221, whole genome shotgun sequence.
ACCESSION   DAOQUC010000006 DAOQUC010000000
VERSION     DAOQUC010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8393
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9403_length_8393_cov_3.391221"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..632
                     /locus_tag="PKH07_00195"
     CDS             <1..632
                     /locus_tag="PKH07_00195"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNT33401.1"
                     /translation="GDERRRRAQVDLLGRADPGRAGQPLQKAVRLNPNRPEGLYWLGT
                     VYYDAGDYEHAIEKFETLLRNHGNYVNTTFNLGTAYARSGQKQKAIELYQEGLRTDPY
                     YAKMHDYLGRCYLLDSTQQEGERLAKEHFEKASDYYLQQIARNPTDFELRVDRGKVLM
                     LLNQREQAQDEFTKALEMNPENAQAKSLLERVRQSPDDTESERRGSSVE"
     gene            complement(692..1588)
                     /locus_tag="PKH07_00200"
     CDS             complement(692..1588)
                     /locus_tag="PKH07_00200"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNT33402.1"
                     /translation="MSRLLQEKDLPAVTLVCNRAMPLDHFFVELLHEKTFGDNNYDKN
                     LNLVEENDGAIVGFASGVVRQRKGEKVGFTKLLAVNPDFREKGIGFQLLSELETKFRE
                     LGCVKARIMDSAPNYFMPGLDYRYTEGTCLLQKAKYTRDGVSLNLIADLQYFPIDHSE
                     EIRQCEEKGVQIRRALPSDKEASLAMVAREWTSWIPEVEATFRHDPVTLFIGLKDNQV
                     VGFSAYDSNNVRTGWFGPMGVLPEYRKFGIGQIVCKLCLAEIKKQGFHTSVIPWVGPV
                     RFYSKVCGARNDRTFWTYEKEL"
     gene            complement(1682..2098)
                     /locus_tag="PKH07_00205"
     CDS             complement(1682..2098)
                     /locus_tag="PKH07_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNT33403.1"
                     /translation="MAETVSRRIVLHFPNEILDKPIVFILVKEFELVFNILKAFVVPD
                     EEGLMVMELSGTSENLQKGLDYLKSRNVRIEPLKQSIVRLDDRCYQCGLCTGVCPSGA
                     LSLKRPSMEVEFDSDKCVLCGACVEICPARAMKIAF"
     gene            2416..3576
                     /locus_tag="PKH07_00210"
     CDS             2416..3576
                     /locus_tag="PKH07_00210"
                     /inference="COORDINATES: protein motif:HMM:NF025665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 99-like
                     domain-containing protein"
                     /protein_id="HNT33404.1"
                     /translation="MKRIGLMSICFSFFLMTGQSAGVHQKPFVMAHYYTWYTTGTGPH
                     AHWGAWGKSEPSLYHPQGASPERVLFPPYVREISSCAYPLVGAYDSDDPEVVRWHIRL
                     AKAAGIDAFLVDWWGPANWQKPSGFTHDVFVKTVLPIAEEENFKVCLFDETPQFAADL
                     SDVKNWIVTYLNQFKDSPAYLHFEGKPVYGIYQHPVGSLTPESATELRDYVEQGVGPI
                     YWIVDKMLCRAVPPEKGDFDLYVPDDWLALSWVDCFMGYSTFGIVRRYEYEDVRRMFG
                     NVVNQVHDKGHKMLLPVHPGHNNSKIATEPWVMPRRDGQTFSDYLRAAEETAPDFIAV
                     TSFNEWPETTVIEPSRSWADPYQYLKILAQWKSIQFIQPEEPRRVTNPEILE"
     gene            3607..>4140
                     /locus_tag="PKH07_00215"
     CDS             3607..>4140
                     /locus_tag="PKH07_00215"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HNT33405.1"
                     /translation="MRFEFATATRIIFGPGTIQQVGPLARQMGHHAFVLADSSLSRHT
                     EEALSQLRSHGVEWTVYDVTGEPAVSTVLDAVLMAKKTRCDLVVAVGGGSVLDMGKSV
                     SALLTNHGDLMDYLEVVGKGEPLRQPSAPCIAIPTTAGTGAEVTRNVVLLIPEQRVKV
                     SMRSPYLLPRLAIVDPEL"
     assembly_gap    4141..4150
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4151..4788
                     /locus_tag="PKH07_00220"
     CDS             <4151..4788
                     /locus_tag="PKH07_00220"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020409600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HNT33406.1"
                     /translation="WTLPPKLTASTGLDALTQLLEAFVCNQPNPLTDSLCREGLRRAA
                     GSLQKAYENGDDHDAREDMCLASLFSGMTLANAKLGAVHGLAGTIGGMFPAPHGMVCA
                     CLLPYVMDTNIRALQDRLKDSPALERYQEFARIVTGKSRSAVSKGLEWVEQLCSSLSV
                     QPLSEYGVTEEDIPAIVEKAMKSSSMKGNPILLTEEELQKTLAKALFFLTK"
     gene            4924..6210
                     /locus_tag="PKH07_00225"
     CDS             4924..6210
                     /locus_tag="PKH07_00225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33407.1"
                     /translation="MQRIPRMLIVLSMLLLVAGHVAALEGYQEPLRVQSGEALIGIYF
                     NGYKASPWKSDDAKVGEDLARIRAMGANALFAVHGPVEAISDDWEAFDREHALAKAAG
                     LSILPGLEIQAGTELSNEKVAQKYSIAVPKAENRNQEKQNSLVTSDEFRDALTRYATD
                     YLDRYLEKGAILRLLDGGKPKPVIALSFETGWLGVSFDKETTTRFWKKIYQHYMASGW
                     NIGTLNFNWGTRIPDFAEINPKDPLIFDYDFILPDDKRLWRPMSEHAMFRADLVNEAL
                     TVVRDRLLEKYPDLLFAAEIPYPFECPARKAIVYKWNAASLPRIVNHADLVLVRTAGD
                     LTGAEQNALLDLIESGRQVVLCHSVAKDFGFGRKLRPIFYEHFVPTLTAQFSNGLGFY
                     SWNEVLSHNFGRDPELVGKAESIAKAYGQIYSATGN"
     gene            6630..7820
                     /locus_tag="PKH07_00230"
     CDS             6630..7820
                     /locus_tag="PKH07_00230"
                     /inference="COORDINATES: protein motif:HMM:NF012470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HNT33408.1"
                     /translation="MTTIAVLFLVWTSTASINCSAYEGTSGDQSDLSFYKPNAVKTAL
                     ERTRNTGVRNVILLIGDGMSLAPMVASQIKALGADGSLNIQKMPITGLSRTNSLDKLV
                     TDSAAAGTALATGYKTRNGMIAALPDGKIPETILEAAKRKGLRTGLVATSTMSHATPA
                     SFAAHQDSRGSEAEISADMLATRVNVLFGGGLKFFLPASVEGSAREDERDLLREAREA
                     GYTFLSNREEMLQADVDYALGLFQKGPLLTNDPSEPSLAEMTQKAIDLLRCKKGFFLM
                     VEGSQIDWAGHENNFDYLARQTLLFDQAVGIALQFALQDHHTLVVVTADHDTGGLGIN
                     NGSLSGDTLELGWTTKSHTGVQVPVYAYGPGSEEFVGVRDNTEIPKVFARLLKIKDFP
                     RLLR"
     gene            complement(8069..>8393)
                     /locus_tag="PKH07_00235"
     CDS             complement(8069..>8393)
                     /locus_tag="PKH07_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015751397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNT33409.1"
                     /translation="MLDKARAAAARLGAHNVEFRLGEIEHLPVADSSVDVILSNCVIN
                     LSPDKPAVFREAFRVLKPGGRLIVPVGAQSSVQSLQRIRKTPTGQIVKEDVIPVRFVP
                     LVRQQ"
BASE COUNT         1992 a         2207 c         2264 g         1920 t
ORIGIN      
        1 ctggcgacga aaggcgtcgc cgggcgcagg tagacctgct cgggcgcgcc gatccgggac
       61 gagcaggaca gcctcttcag aaagctgttc ggctcaaccc aaatcgtccc gaggggttgt
      121 actggctcgg aacagtttac tatgacgctg gcgactatga gcatgccatc gagaagttcg
      181 agacactgct tcggaatcat ggcaactacg tcaacaccac tttcaacctt ggcaccgcct
      241 acgctcgaag cggtcagaaa cagaaggcaa ttgagctcta tcaggaaggc cttcgaacgg
      301 atccctacta cgccaagatg catgattacc ttggccgctg ttatctgttg gattcgactc
      361 aacaggaagg ggagcgtctt gccaaagagc attttgagaa ggcatcggat tattaccttc
      421 agcaaattgc cagaaatccg actgactttg aactgcgtgt ggatcgggga aaagtcctga
      481 tgctcctgaa ccaacgagaa caagcgcagg atgagttcac gaaggcactt gagatgaatc
      541 cggaaaacgc tcaggcaaaa agccttctgg aaagagtcag acagtctccc gatgacacgg
      601 agtccgaaag acgcggatca tctgtcgagt aggtgcctga agaagtggac acagcccatg
      661 agcctgtagc tgacagtctg ctcagtggcg actacagctc cttctcatag gtccagaaag
      721 tccgatcgtt tcgggcaccg cataccttcg agtagaaacg caccgggcct acccagggaa
      781 ttacggatgt gtgaaatccc tgcttcttga tctccgccag acagagtttg cagacgatct
      841 gtccaatgcc gaatttacgg tattccggca acacgcccat cgggccaaac cacccggtac
      901 gaacgttgtt gctgtcatag gccgagaaac ccacgacctg gttatctttc aatccgatga
      961 agagcgtcac gggatcatga cgaaacgtgg cctccacttc tggaatccag gacgtccatt
     1021 cccgagcgac cattgcaagc gatgcctctt tgtccgaggg gagcgctcgt cggatttgaa
     1081 cccccttctc ttcacactga cgaatctcct cggagtggtc gatcgggaag tactggagat
     1141 cggcaatgag gttcaggctg accccgtcgc gggtgtattt ggctttctgc agcaggcaag
     1201 ttccttccgt gtagcgatag tccaaacccg gcatgaagta gttcggagcg gagtccatga
     1261 ttcgcgcttt gacgcaaccc aactcacgga acttcgtttc caactcactg agaagctgga
     1321 agccgatacc cttctcccga aaatcgggat tgaccgccag aagtttcgtg aatccgacct
     1381 tctccccctt gcgctggcgc acgacaccac tggcaaaccc gacgatcgca ccatcatttt
     1441 cttcgacaag gttcagattc ttgtcgtaat tgttatcgcc aaaggtcttc tcgtgcagca
     1501 gttcgacaaa gaaatgatcc aacggcatcg cgcggttgca gactaacgtc acagcgggca
     1561 agtccttctc ttgaagcaga cgactcattt ttcactacct cccggaaaaa ccaatatggg
     1621 cattgtggaa caagtgtatc tgagaaccga acatgaggca agccgtactc atgtccaaac
     1681 atcagaatgc gatcttcatg gcgcgcgcgg gacagatctc cacgcaggca ccgcacaaca
     1741 cgcatttatc cgaatcaaac tcgacttcca ttgaaggcct cttgagggaa agcgcaccgg
     1801 atggacacac gcctgtgcat agaccacatt gatagcatcg gtcatccaac cggacaatgc
     1861 tctgtttgag cggctcgatg cgcacatttc ggcttttgag atagtccaac cccttctgaa
     1921 gattttccga tgttcccgac aactccatga ccatcaagcc ctcctcgtcg ggaacgacaa
     1981 aagctttcag gatattgaag accaattcga attctttgac cagaatgaag acgatcggtt
     2041 tgtccagaat ctcgttcgga aagtgaagaa caattctcct actgacggtc tcggccatgt
     2101 ttatagactc ctgggctccc ccctatcact cccctgcgga cgggggagaa cctctgaagt
     2161 cccttccttt ccagggaggg cagggggagg tttcttttct gtcgtgtgtg cctgctgctg
     2221 cacagcccaa caagtcacgc acaatagcac ttacaggcac ttcaggcaaa taggccgtga
     2281 aatgtggatc cggtgcagac cagaattgac gggttacgct gatttggctt tccttctcga
     2341 cttatgctac tatccaaagt agagagttcc gacgtcctgc gttcgtcggt ttgaatccta
     2401 aggatccatg ggacaatgaa gagaattgga ctgatgagca tttgtttttc cttcttcctg
     2461 atgacagggc agtctgctgg agttcatcag aaacctttcg tcatggcgca ttattacact
     2521 tggtacacga cgggcacggg gcctcacgct cattggggag cttggggtaa gagtgaaccg
     2581 agtctctacc acccacaagg tgccagtcct gaacgcgtac tcttcccgcc ctacgtgcga
     2641 gagatttcca gttgcgccta tcccttggtt ggtgcttacg acagcgatga tcctgaagtg
     2701 gttcggtggc atatccgact ggcgaaggca gctggcatag atgcctttct tgtggactgg
     2761 tggggacccg caaactggca gaagccttcg ggctttacac atgacgtctt tgtcaaaacg
     2821 gtgcttccca tcgcagaaga agaaaacttc aaagtgtgtc tttttgatga aactccccag
     2881 ttcgcggccg acctcagcga tgtcaagaac tggatcgtca cttatctcaa tcagttcaag
     2941 gacagcccag cctatttgca ttttgaaggt aaaccagtct acggcatcta ccagcatccc
     3001 gtgggaagcc tcacgcccga gagtgctacg gagcttcggg actatgtcga gcagggagtt
     3061 ggtccgattt actggatcgt ggataagatg ctttgtcgcg cagtgcctcc cgagaagggg
     3121 gatttcgatc tctatgttcc cgatgattgg ctcgcccttt cctgggtgga ctgtttcatg
     3181 ggatattcca ccttcggaat agtccgccga tatgaatatg aggatgttcg gcgaatgttt
     3241 ggcaacgtgg tgaaccaggt tcacgacaag ggtcacaaaa tgcttctgcc tgttcatcca
     3301 ggacacaaca acagcaagat tgctaccgaa ccttgggtga tgccgagacg ggacggacag
     3361 actttttcgg actacctcag agccgctgaa gagactgctc cggacttcat tgctgtgacg
     3421 agtttcaatg aatggcctga gaccacagtc attgaaccat cgcgttcgtg ggctgatccg
     3481 tatcagtacc tcaagattct tgctcagtgg aaatctattc agtttatcca acctgaagag
     3541 ccaagacgag tcaccaaccc cgaaattctc gagtgattcc gtccgcacct gaaggaggaa
     3601 caaatcatgc gttttgaatt cgccacagcc actcggatca tattcggtcc cggaaccatc
     3661 cagcaagtgg ggccgcttgc caggcagatg gggcatcatg cctttgttct ggcggattca
     3721 tcactgagcc gtcacaccga agaagcgctt tctcagttgc ggagtcatgg tgtggagtgg
     3781 acggtctatg acgtgaccgg agagcctgcc gtgtcaacgg ttctggatgc agtcttgatg
     3841 gcaaagaaga cgcgctgcga tttggttgtg gcggtcggag gcggtagcgt tctggatatg
     3901 ggcaagtcgg tttcggcatt gctgaccaac catggcgatc tgatggatta tctcgaagtt
     3961 gtcggcaagg gggagccact tcggcaacct tccgcaccct gcatcgcgat tccaactacc
     4021 gctgggacag gtgccgaggt aacaaggaat gttgtgcttc tgatccccga acaaagagtg
     4081 aaggtcagca tgagaagtcc gtatctgttg cctcgcctgg ccatcgtgga tccagagttg
     4141 nnnnnnnnnn cctggactct gccaccaaaa ctgacggcaa gcaccggact ggacgcgctt
     4201 acacagctgc tggaagcctt cgtatgcaat cagcccaatc cgctcacaga ctctctttgt
     4261 cgtgagggtt tgagacgggc agccggttca cttcagaaag cctatgagaa cggcgacgat
     4321 cacgatgcgc gcgaggatat gtgccttgcc agcctgttca gtggcatgac tctggcgaat
     4381 gccaaactcg gcgcggtcca tggtctcgcc ggaactattg gtggtatgtt tcccgcgccc
     4441 catggcatgg tttgcgcatg tctgctgcca tacgtcatgg acaccaacat ccgtgcgctc
     4501 caagaccgac tgaaggattc accggcgctg gaacgctatc aggagttcgc tcgaattgtg
     4561 acgggcaaat ccagatctgc ggtaagcaaa ggacttgagt gggtcgagca actctgttcc
     4621 agcctgagcg tccagccact ctccgagtac ggggtaacgg aagaggatat tccggcgatt
     4681 gtcgaaaagg cgatgaagtc cagcagcatg aagggcaatc cgattttgct tacagaagag
     4741 gaacttcaga agactctcgc aaaggcgctt ttcttcttga ccaagtagaa ttgctctgtc
     4801 ccatgccttg gcgagcgtgg aagagcacac cgtcttgacg aacaagacgt ttgacgcgta
     4861 ggatcatcct gtacagaaag cgatcttcgg gaaccggatg tgttccccga tggagacagt
     4921 ttgatgcaaa gaattcctcg aatgttgatt gtcttgagta tgcttcttct tgttgcgggc
     4981 catgttgctg ccttggaagg ttatcaagaa cctctccgag ttcagtctgg tgaagcgttg
     5041 atcggaatct acttcaacgg ctacaaggcc tctccgtgga aatccgatga cgcaaaggtc
     5101 ggtgaggatt tggcacgcat tcgagcgatg ggagccaatg cgctttttgc agtgcatggt
     5161 ccggtcgaag cgatttcaga tgactgggag gcatttgaca gagagcatgc gctcgcaaaa
     5221 gcggcggggc tctccatcct tccagggctg gagattcagg ctggaacgga actgtccaac
     5281 gagaaggtcg cgcagaaata cagcattgcc gtgcccaagg cggagaaccg aaaccaggag
     5341 aaacagaaca gcctcgtcac ctccgatgag tttcgagatg ctctgactcg ctacgcaacc
     5401 gactacctcg accgatatct tgaaaagggc gccattctca gactgcttga tgggggaaag
     5461 cccaaaccgg tgatcgcgct gtcttttgag acaggctggc tgggtgtctc gtttgacaag
     5521 gaaaccacga ctcgtttctg gaagaagatc taccagcact atatggcaag cggatggaac
     5581 atcggcacgc tgaactttaa ctggggcacc cgaatcccag actttgctga gatcaacccc
     5641 aaggatcctc tcatctttga ctatgacttc atactgccag acgacaaacg gttgtggaga
     5701 cccatgtcag aacatgccat gtttcgggca gacttggtta atgaagccct caccgtcgtt
     5761 cgcgaccgac tgcttgagaa gtatcccgat ctccttttcg ccgcagaaat tccgtatccg
     5821 ttcgaatgtc ctgctcgcaa agcaatagtg tacaagtgga acgctgccag tcttccccga
     5881 atcgtaaatc acgccgatct tgtgctggtt cgaaccgcgg gtgacctcac gggagcagaa
     5941 cagaacgcac tgctggattt gatcgaatcg ggacgacagg tagtgctgtg tcattcggtt
     6001 gcgaaggatt ttgggtttgg cagaaaactc agaccaatct tttacgaaca cttcgttcca
     6061 accctgacgg ctcagttctc caatggcttg ggcttctaca gttggaatga agtcctctcg
     6121 cacaactttg gacgcgaccc agagctggtt ggcaaagccg aatccatagc gaaagcctat
     6181 gggcagatct actcagccac gggcaactga ggttgtccaa gaccactctt ttcaacacca
     6241 acgagattcc aaacagaatc ttaaccaatt caatcgtaac gttttgaggc tttgtacgtt
     6301 attaagccaa gtaccccaat aatggggtgc aaggaggtaa aggttagtat atggacagcg
     6361 atccgagtag ttgggagcga acattcactt tgttgaagtc gttcctgaac gagcagtcgg
     6421 agaaagagtc ggaagaatgc gaaacaaccg cggttcagtt cgctctttca gggtcaccga
     6481 aattgtcaag ttggcaggtt cccaactggg gaagccaggg attggacgag gctggcttga
     6541 cgatgatatc caacgattac tggaacatac tataataatg tggaggctcc ctagtggaga
     6601 caaaggcatg tagaagaagc ctgtttacga tgacaacaat tgcggtgctc ttcctggtct
     6661 ggacgagcac cgcttccata aactgttcgg catacgaagg cacctctggc gatcagagcg
     6721 atctgagttt ctacaaacca aatgctgtca agactgcctt ggagcgaacc cgaaacaccg
     6781 gtgtgcgcaa tgtcatactt ctcattggag atgggatgag tttggcgccg atggttgctt
     6841 ctcagatcaa ggcgttgggt gcggacggtt ccttgaacat ccagaagatg ccgatcactg
     6901 ggctttccag aacaaactca ctagataagc tggtgaccga ttctgccgcg gcgggaactg
     6961 cgctcgcaac cgggtacaag acccgcaatg gcatgattgc ggcgctgcct gacgggaaga
     7021 ttcccgagac cattcttgag gccgccaaac gcaaaggatt gcggacgggt ttggtggcaa
     7081 cgtccaccat gagtcacgcg actcctgcgt cctttgcagc gcaccaggat tccagaggca
     7141 gtgaagcaga gatttccgcg gacatgttgg cgaccagagt caacgttctc tttggcggag
     7201 gactgaagtt ctttcttcct gcgtcggtgg agggcagtgc ccgagaggat gaacgagatc
     7261 ttctaagaga agctcgagag gccggttaca cgtttctctc caaccgtgag gaaatgctcc
     7321 aagccgatgt ggactatgct ctggggctct tccagaaagg gcctcttctt accaatgacc
     7381 cttcagagcc cagtttggct gagatgacac agaaggccat tgatctcttg cgctgcaaga
     7441 aaggcttctt cttgatggtt gaaggaagtc agattgactg ggctggtcac gagaacaact
     7501 ttgattatct tgctcgacag acactgctct tcgaccaggc ggtcggcatc gcgcttcagt
     7561 ttgccctgca agatcatcac acgttggtcg ttgtcacagc ggatcatgac actggtggct
     7621 tgggcatcaa caatggctcg ctgtcgggtg acacactgga actcggctgg acaacgaaaa
     7681 gccacaccgg cgttcaggtt cccgtgtacg cttacggtcc tggctctgag gaattcgttg
     7741 gagtccgcga caacacagag attcccaagg ttttcgcgcg tctgctgaag atcaaggatt
     7801 tcccgcgact actccgctga tcctgatgca ctgactaacc taatcggctc agagaaactt
     7861 gcttcggaaa tcgcagggtt tcatgaatgg atgctacgca tccttcagac aaacaatgac
     7921 ccttatgccg aaggcttcgc ccaattctgc aaaatgcagt gaaggtcttt tctatgtatc
     7981 agcgcggatt ctcagattct ccataggtgc atcgtcgtct ccctttgcgg caagggagcc
     8041 gttcctgccg caccgtgcaa ggccatcgtc actgctgtct caccaagggc acaaatcgga
     8101 cagggatgac atcttctttc acgatctggc ctgtcggtgt tttgcgaatc cgctgaaggc
     8161 tttgcactga tgactgggct cccacaggga cgatgagccg tccaccgggt ttcagcacgc
     8221 gaaacgcttc gcggaaaact gccggcttgt cggggctgag gttaatcacg cagttggaca
     8281 ggatcacatc cacgctggag tcggcgacgg gcaggtgctc aatctccccc aggcggaact
     8341 cgacgttgtg tgcgcccagc cgggcggctg cggcccgcgc cttgtcgagc atc
//
LOCUS       DAOQUC010000007         8181 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_9706_length_8181_cov_3.811962, whole genome shotgun sequence.
ACCESSION   DAOQUC010000007 DAOQUC010000000
VERSION     DAOQUC010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8181
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9706_length_8181_cov_3.811962"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..465)
                     /locus_tag="PKH07_00240"
     CDS             complement(<1..465)
                     /locus_tag="PKH07_00240"
                     /inference="COORDINATES: protein motif:HMM:NF014235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HNT33410.1"
                     /translation="MPLADILSQEEIDALLSALSSGEVAETEVEETPTKTKLVKTWDF
                     RRPDRFSKDQTRTLQMLHEQFARYFSGLLSMHLRSLVEVKLTSVDQLTYDEFIRSLPN
                     PTCLAILNMTPLEGQAMLEISPVISFSFVDRMMGGQGAEFTRNRELTEIEQAL"
     gene            complement(502..1023)
                     /locus_tag="PKH07_00245"
     CDS             complement(502..1023)
                     /locus_tag="PKH07_00245"
                     /inference="COORDINATES: protein motif:HMM:NF015693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HNT33411.1"
                     /translation="MAEEAQVAQTPSKPKRKVLLIAVVGVVVICAAIIFGGLYFIKST
                     TPGESDPNAATPVPLAGDTVKPLETKVLGSFVVNLAPPDESIFLAVDIVLAYRGDDKK
                     QTEMLSAEVDARLPQIKHHVNMILSGKRKKDVSTPDGKERLNREILKKVNSLLTAGQI
                     EEIYFDKIVTQGS"
     gene            complement(1269..1679)
                     /locus_tag="PKH07_00250"
     CDS             complement(1269..1679)
                     /locus_tag="PKH07_00250"
                     /inference="COORDINATES: protein motif:HMM:NF017020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HNT33412.1"
                     /translation="MRDLDQSLSLLEMSWKDFKALCGMLDADVFDQEIFGFHVQQAAE
                     KAFKSLLALNVGEYPKVHDLRLLIRELQNAGADIAPFLSLLEFNTYAVQFRYEALGAP
                     DEPIDRENVIREIRLLLEQVQRAVADACAKDGTR"
     gene            complement(1666..1998)
                     /locus_tag="PKH07_00255"
     CDS             complement(1666..1998)
                     /locus_tag="PKH07_00255"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HNT33413.1"
                     /translation="MTKSDRLLDDIVATIVKEVSPEKVILFGSHASGTTHPDSDIDLL
                     VVESEPFGAERSRRKETARLSLALLPFRVPIDLLVFARDEIDRWKDSANHVVARALRE
                     GRALYERP"
     gene            complement(2390..3775)
                     /locus_tag="PKH07_00260"
     CDS             complement(2390..3775)
                     /locus_tag="PKH07_00260"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNT33414.1"
                     /translation="MKPNIVFILVDDMGYGDFSCINGGLSQTPSLDQLMTESVCFSQQ
                     YTASPVCNPSRACLMTGRYPHRTGSIDTLEWRGLERLALRETTLAQVLKNAGYVTGLV
                     GKWHLGAFDMRYHPMNRGFDETVCFRGGMHDYYNWRLEYDETVVRADGRYLTDVWTQE
                     AVSFIERHQKEPFFLHLTFNAPHTPLQAPEEDVRPFLEMGRYTKGVSTLYGMLRRMDA
                     GVGRIMETLRSYGLEDNTLVLFTSDNGPQFGGEGENCLNRFNCQLRGAKCSVYEGGIR
                     VPMILRWPAGVEGSRESNVMIHSSDWFPTILSMVGVRLPHELRIDGVDISPVIRGEEG
                     KTCTKRFWQWNRYTPLVTCNAAVRDGDWKLVRPTIPEAMACPCVDPWLRISMYEPERL
                     IQNGLLQEPEPERQIPDPPQPELYNIREDPLEQSNLIDQYPDVAHRLLCDLENWFEEV
                     ERERATIPDVW"
     gene            complement(3795..5540)
                     /locus_tag="PKH07_00265"
     CDS             complement(3795..5540)
                     /locus_tag="PKH07_00265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33415.1"
                     /translation="MRSATRFRRLPLILPLLGFVLATLLLTYPLAFRLSDFINDVPAV
                     DHGANYWNLWWAERWVSSSETQLLYTDYLFHPEGVPLVFHTLTVLNGLLSIPIQRLFG
                     LTVAYNLIVLLSFVLTGLATYLLARHWGAERLSGFVVGLWFAFSVYRFHHWDHLNLLS
                     THWLVFFLWALSRFLHSARLKDALLTGVFWAATGLCSWYYLVFAGTSGVLLCVCVALR
                     QGRALQIRRWLLGCLIVTGVFLLIMSPYVSAILKARQDAPPDDLSFAVSAYFSVDVLC
                     LLLPHTLLSPFVNRAGLFAFTAECPEAIFPTTTVLLALLGLILMPKRLPRALPWEWIG
                     MGSVFALLSLGPFLKVGGLVQMGEMGYVLMPGYLFKKMPMMGFFKVFDRFLLVTHLGL
                     MVFVAPFLGFLIARLAEWLRLRPTSIWAVVIVLSVAQGWHGGISMTDKKPSDFFTQVA
                     SETGDFSIVEVPFDTEAYPSHGRAMFHQTIHHKRTINGEVSRPPKSLHTRLERNLVIS
                     LLKKYYEVEQYPVSEEDRIRFLEDAHSLRLRYVVLYPERLRIGYDQTLEEFLVGTLNG
                     TMVYTEPGMRVFRIE"
     gene            complement(5660..6751)
                     /locus_tag="PKH07_00270"
     CDS             complement(5660..6751)
                     /locus_tag="PKH07_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015847479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HNT33416.1"
                     /translation="MSIVPGNAMANLTLAKDFLPARVSSWDTSGRNRDSWRIEPGESR
                     VLAEIDGPGVISHIWFTISSPDKHYLRKLLLRIFWDGEENPSVESPVGDFFGLGHSRR
                     FSYQCAPFNTSSAVEGEIGGGVALNCWFQMPFRRKAKVVLVNEQEEPVNAFYFYVDYQ
                     KHKALNEETVYFHAQWRRNNPCDGWTGEGSVWHSAAWGQRIGGEEGVNLSDEKNYLIL
                     EAEGRGHYVGVNMSIDNLYKGWWGEGDDMIYVDRDGQRAWPPDMHGTGSEDYLSHAWG
                     MQKNAHLYNGLSWAERESDYNYAGKVCVYRYHILDPIPFQKNIRVSIEHGEANVRSDD
                     YSSVAYWYQNEPHKAFRPILSAKLRLPNI"
     gene            complement(6985..>8181)
                     /locus_tag="PKH07_00275"
     CDS             complement(6985..>8181)
                     /locus_tag="PKH07_00275"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNT33417.1"
                     /translation="GQWHNVGRLRDVEETLLHPLKQLGYRTCHVGKSHLHPHGSGRDL
                     RDAIPFMNALGWDDVLECTGPWSTRTTTSILTDWMKTEGIFDLFMEDYARRKQAGVKN
                     LWPSPLPDGKHPDDFIARTAVNYIHDSDPSAPLYLFVGFGGPHEPWDSPVRFDTYQPE
                     DMPPPLPRDGVPDWLSNPARDFQERTTSHNPSVTENDWARIRTLYSGRVEHVDSCLGE
                     VLKAWYQKRGQDTWVVFWADHGEMLGDKAHSGKCVFYESSVRVPAIIRPPQGCSSPIV
                     SNALVGLTDLTATVLDAAEYTSLSQNIFGHSLLGVFENSDSVGSPYVVSEIGHSTMIF
                     DGIWKLVVDSRDETLQFFNLQADPTESLNLAGKPDLSEVEKHLRASLRHFLLRTQYRQ
                     HREKNG"
BASE COUNT         2044 a         2255 c         2034 g         1848 t
ORIGIN      
        1 taatgcctgt tcgatctcag tcagttccct gtttcgggtg aactccgcac cctggccgcc
       61 catcattcga tccacaaacg aaaatgagat aaccggcgat atctctaaca tcgcttgtcc
      121 ctcaagtggt gtcatattca ggatcgccag acaggtcgga ttcggcagcg atcgaatgaa
      181 ctcatcatag gtcagctgat cgacagacgt gagtttgacc tcgaccaggc tcctcaggtg
      241 catggacaaa aggcccgaaa aataccgcgc gaactgctcg tgaagcattt gcagggttcg
      301 agtctggtct ttggaaaatc gatcgggccg acggaaatcc cacgtcttga ccaacttggt
      361 cttcgttggt gtttcctcga cctcggtctc cgccacttca ccagaggaca atgccgacaa
      421 tagggcatct atttcttctt gtgacagaat atcagccagt ggcaccgcct ccttcccgtt
      481 gggcgaacca gaatcccgtc atcatgaacc ttgagtcaca atcttatcga agtaaatctc
      541 ctcgatttgg ccagccgtca gcaacgaatt cactttcttc agaatctccc gattcagtcg
      601 ctccttgccg tcgggagttg aaacatcctt cttcctctta cccgaaagaa tcatgttgac
      661 gtggtgtttg atttggggaa gccgtgcatc cacctcggcc gacagcattt ccgtctgctt
      721 cttatcatca ccacggtatg ccaacacaat atccaccgcc agaaagatgc tttcgtccgg
      781 cggggcaagg ttgacgacaa aacttcccaa aacctttgtt tccaaaggct ttacagtatc
      841 accggcaagc ggaacaggcg tggctgcatt cggatctgat tcgcccggag ttgtgctctt
      901 gatgaagtag aggccgccga agataatcgc ggcacagatc accacgacac caaccacggc
      961 gatcagaagt acttttcgct tgggcttgct tggagtttga gctacttgag cttcttcagc
     1021 catacttcgc tctccttctt aagtcaaacg ctacaacctt tgacagaata agatattata
     1081 ggatgtgaag cgcctctgtc aagcagataa aacgaggctc tgaaaccagt cagttggtgg
     1141 gctcaaacaa gcgaatctgc gacagcaact gaggccattg tacctttctt ctggtattgc
     1201 gtcggcgaaa acggcggaac gcgctcggtc acggagcaga cttcgtctgt tccaccccgc
     1261 aagttccctt acctcgtgcc atccttagcg caagcgtctg ccaccgctcg ctgaacctgt
     1321 tccaagagca aacgtatttc acgaatgaca ttttctcgat caatcggttc atcaggggcg
     1381 cccaaagcct catatcggaa ctgcacagca taggtattga actcaagcaa actcagaaag
     1441 ggggcaatat ctgctccagc attctgcagc tcgcgaatga gaagacgaag atcgtggact
     1501 tttgggtatt cccccacatt gagagccaac aaagatttga aagccttctc agcggcctgc
     1561 tgaacatgaa acccgaatat ctcctgatcg aacacatcag catcaagcat tccgcagagt
     1621 gctttgaaat ccttccagga catctcgagc aaggagagag attgatcaag gtctctcata
     1681 caacgctctc ccttcccgaa gcgcccgtgc aacaacgtga tttgcagaat ccttccaacg
     1741 atctatctca tccctcgcaa acacaagcag atcgatcgga acgcggaaag gcagaagcgc
     1801 gagcgacagg cgggcagtct ctttccttct gctcctctcg gcgccaaaag gttcggactc
     1861 caccacgagc aggtctatat cggaatcggg atgtgttgtg cccgatgcgt gggagccaaa
     1921 caatatgact ttttcagggg aaacctcttt cactatagtc gcgacgatat catcaagaag
     1981 tctgtcgctc ttagtcatct ctgttcctcc aatgctttga ctcatccaca agacgcgtca
     2041 ctatgcagaa acaatccagt atccgccaca gcaaactgca gttcgccggg ttgaataaat
     2101 aaagtagaac gagtttctcc tgcccctcgg ttgaaccgca cctgaatctg ctggtctggt
     2161 acatcagact atgtctcgct ccgaccagtt cgtagcactt ccaaggtact atgacagttc
     2221 aaatactctc tgtcatctgc gaattcaatg taaacgagtt acgctgggaa gtcaccatct
     2281 tctttcgccc caagagcact ttctagtctg ggttggattt gcaggctttc ctctcgtccc
     2341 ttaaccgaag ttcctctaac tatcattccc tcctggggta aaatcaattc taccagacat
     2401 ccggaattgt tgctctttct ctctccactt cctcaaacca gttctcaaga tcgcagagca
     2461 atctgtgagc tacgtcagga tattggtcta tcaggttgct ctgctcaagc ggatcttcgc
     2521 ggatattgta caattcgggt tgcgggggat ccggaatctg gcgctccggc tcgggttcct
     2581 ggagaaggcc gttttggatc agtctttctg gctcatacat ggaaatccga agccaagggt
     2641 cgacacaggg acaagccatt gcttcgggaa ttgttggacg gacgagtttc cagtctccat
     2701 cacgcaccgc agcgttgcat gtaaccaacg gtgtgtagcg attccattgc cagaaacgct
     2761 ttgtgcatgt tttgccctct tcacctcgaa tcactggaga gatgtccact ccatcaattc
     2821 tgagttcatg aggcaacctc acaccaacca ttgagaggat ggttggaaac cagtcgctgg
     2881 aatggatcat gacgttgctc tcgcgagaac cctccactcc tgccggccaa cgaagaatca
     2941 tgggcacacg aattccacct tcgtagacgg agcacttggc accacgcaac tgacaattga
     3001 accggttcag gcagttttct ccttcaccgc caaactgagg accgttgtcg ctggtgaaaa
     3061 ggacaagagt gttgtcttca agaccgtatg atcgcagcgt ctccatgatc cgccctactc
     3121 cggcgtccat gcgccgcaac attccgtaga gtgtgctgac tcctttcgtg tagcgcccca
     3181 tctcaagaaa gggtcggacg tcttcttccg gagcctgaag aggtgtgtgg ggagcattga
     3241 aggtcagatg aaggaagaaa ggctccttct gatgccgttc gataaacgag accgcctctt
     3301 gtgtccagac atcggtcaga taacgtccat cggctctcac gacggtttcg tcatactcca
     3361 gtctccagtt gtagtagtcg tgcattcccc ctcgaaaaca taccgtttca tcaaagcctc
     3421 ggttcatagg atggtagcgc atatcaaacg cgccgagatg ccacttgccg acaagtcctg
     3481 tcacatatcc ggcattcttc aacacctgcg ccagagttgt ctcgcgcagt gccaatcgct
     3541 ccaaaccacg ccattccaat gtatctatgg agccggttcg atgtggatag cgccccgtca
     3601 tcagacatgc ccgagatgga ttgcagaccg gcgatgcggt gtattgctgt gagaagcaga
     3661 cactttccgt catgagttga tccaaagatg gagtttggga cagcccgcca ttgatacaac
     3721 tgaagtcacc ataccccata tcatcaacca ggatgaagac gatgttaggt ttcataatgc
     3781 ctcctcaggt caagtcactc aatcctgaat acccgcattc ctggctcggt atagaccatt
     3841 gtgccattga gtgtaccaac cagaaattcc tcaagcgttt gatcatagcc gattcgcagt
     3901 cgctcggggt acagcacgac ataacgaagc cgaagactgt gcgcatcttc gagaaatcgt
     3961 atgcgatcct cttctgagac aggatactgc tcgacttcat agtacttctt caacaacgag
     4021 ataacaagat ttcgctcaag ccgagtgtgc agactcttgg ggggacgtga aacttcacca
     4081 ttgatagtgc gtttgtgatg gatggtctgg tggaacatgg cgcgaccgtg tgagggatag
     4141 gcctctgtgt cgaatggaac ctcaacgatg gaaaaatcac ccgtctcgga agccacctga
     4201 gtgaagaaat ctgagggctt cttgtcggtc atcgagatgc cgccgtgcca tccctgagcg
     4261 acactcagca caatcacaac cgcccagatg ctggtcggtc gaagtcgtaa ccattctgcc
     4321 agtcgggcaa tcagaaagcc cagaaatgga gccacaaaaa ccatcaaacc aagatgtgtg
     4381 acaagcaaga accgatcgaa gactttgaag aatcccatca taggcatctt cttgaaaaga
     4441 tagcccggca taagaacata gcccatctcg cccatttgaa ccaacccacc caccttgagg
     4501 aaaggtccca aagagagaag tgcaaaaacg ctccccatac cgatccactc ccacggcagc
     4561 gccctcggaa ggcgttttgg catgagaatc agccccaaga gtgcgagcaa gaccgtcgtc
     4621 gtggggaaga tcgcttcagg gcattctgct gtgaatgcaa acagacctgc ccgattcaca
     4681 aatggagaga ggagagtatg aggcagcagc aaacagagaa catctacgga aaaataggcg
     4741 gagaccgcaa acgaaagatc gtctggtggc gcatcctggc gggctttgag gatggcactt
     4801 acatagggac tcatgatcag tagaaagaca cctgtcacga tcaggcatcc gagaagccac
     4861 cgtctgattt ggagtgcacg cccctgcctg agagcaacgc acacacacaa caagaccccg
     4921 cttgtcccgg caaacacaag gtagtaccag gagcagagtc cggtggcagc ccaaaacact
     4981 ccagtcagca aagcatcctt caatcgtgcg ctgtgcagaa aacgcgaaag cgcccagaga
     5041 aagaacacca gccagtgcgt actcagaaga ttcagatgat cccaatgatg gaatcggtac
     5101 acgctgaaag caaaccacaa gccaacaaca aatccactga gtcgctcggc tccccagtgc
     5161 cgagccagaa gataggtcgc cagaccagtc aggacaaaag acagcaagac aatcagattg
     5221 taggcaaccg tcagcccgaa aagtctctgg atgggaatcg agagtaggcc attgagcacc
     5281 gtcagtgtat gaaagacaag gggaacgcct tcaggatgga agagataatc ggtataaagc
     5341 agttgggttt ccgatgaact gacccagcgt tcagcccacc acaggttcca gtagttggct
     5401 ccatgatcca cggcaggcac atcgttgatg aaatcactga ggcgaaaggc gaggggatat
     5461 gtcagaagca aggtcgccaa gacaaaacca aggagaggca ggatcaaagg gagtcggcga
     5521 aatcgtgtcg ctgatctcat gaaattgctc ttcgccttct gtcaaatcaa cagaagtgcc
     5581 taatcgtgat cacgtgggac acgtcgtgcg aatcccccga agcctgtttg cacgggggat
     5641 tcgcacacgc actcttgccc tagatattcg gcagtctcaa tttcgccgaa agaatcggac
     5701 ggaacgcctt gtgaggctcg ttctgatacc agtacgcaac tgaggaataa tcgtcactac
     5761 gaacgttggc ttctccatgc tcgatggaga ctcggatgtt tttctggaac ggaatcgggt
     5821 caagaatgtg atagcgatag acgcaaacct tacccgcata gttgtagtcc gactcacgct
     5881 ccgcccagga gagcccgttg tacagatgcg cgttcttctg cattccccag gcgtgcgaaa
     5941 ggtagtcctc actgccggta ccgtgcatat cgggtggcca cgcccgttgt ccgtcgcgat
     6001 ccacatagat catgtcgtcg ccttcgcccc accagccttt gtacaggttg tcaatggaca
     6061 tattgacgcc gacgtagtga ccacgaccct cggcttcaag aatgaggtag ttcttctcat
     6121 cgctgagatt gactccctct tcgccaccga tgcgctgtcc ccatgctgcg gaatgccaga
     6181 cactgccttc tcccgtccag ccatcacagg gattgtttcg acgccattgg gcgtgaaaat
     6241 agacggtctc ttcgttcagt gccttgtgtt tctgatagtc cacatagaag tagaatgcat
     6301 tgactggctc ttcctgctca ttcacaagaa cgacctttgc ttttctgcga aaaggcatct
     6361 ggaaccagca gttcagagcg acgccacccc caatctcccc ctcaactgcc gaactggtgt
     6421 tgaaaggcgc acactgatag gaaaatcgcc ttgagtgtcc cagaccaaag aaatcgccaa
     6481 ccggcgattc gacagatgga ttttcttcgc cgtcccagaa aatgcggaga agcaatttcc
     6541 gcaggtaatg cttgtctgga gacgagatag tgaaccagat atgactgatc acaccaggtc
     6601 cgtcaatctc cgccagcact cggctttctc cgggttcaat tctccaggag tcccgattcc
     6661 ttccgcttgt gtcccagctg ctcacacgag ccggcagaaa gtcttttgcc agagtcagat
     6721 tcgccattgc gtttccaggt acaatactca ccgctttcct cctcttcatt ggcaccatct
     6781 cagtgcctct cttgcagcct ggactctcat cccgaagggg atctcatagg gtaccggctg
     6841 cagaatatga aaccggaata taatccctga catggaactc tgcaagtgcg gttgaaaagt
     6901 gcgaacgata ccagggttcg aatgtgtgga gcgcgatcgg tccgagagtc tcgtcgcttg
     6961 gcagacactg caggacatcc cgcgctaacc gttcttctcg cggtgttgtc ggtattgcgt
     7021 tctgagcagg aagtgacgca gactcgctct gagatgcttc tcgacttcgg agagatcggg
     7081 tttgcctgcc aggttgaggg actcggtcgg atccgcctgc aggttgaaga actggagtgt
     7141 ctcatcacga gagtcaacga ccagtttcca tatcccgtca aagatcatgg ttgaatgccc
     7201 gatctcgctg acgacatagg gcgatccaac actgtcggag ttctcaaaaa cacccagcag
     7261 tgagtgacca aaaatgttct ggctcaaaga tgtgtattcc gcggcgtcca gaaccgtcgc
     7321 cgtaagatcg gtcagaccga caagcgcgtt ggagacgatt ggactcgaac atccctgtgg
     7381 tggtcgaatg atggcgggaa cacgcactga actttcgtag aaaacgcatt tgcccgaatg
     7441 ggctttgtct ccaagcatct caccatgatc tgcccagaag acgacccagg tatcctggcc
     7501 gcgcttctgg taccaagctt tgagcacttc tccgagacaa ctatccacgt gttcgacacg
     7561 tccggagtac agggtgcgta ttcgtgccca gtcgttctct gtcacgcttg ggttgtggct
     7621 ggtggtgcgc tcctgaaaat cgcgtgccgg attgctcaac cagtcgggaa caccgtcgcg
     7681 gggcaacggt ggtggcatat cctcgggttg ataagtgtcg aaccgcacag gggaatccca
     7741 cggctcatga gggcccccga aaccaacaaa aagatagagt ggggccgatg ggtcagaatc
     7801 gtggatatag ttcacggccg ttcgagcaat gaagtcatcg ggatgcttcc catcgggaag
     7861 aggtgatggc cagaggttct tcacgccggc ctgtttgcgt ctggcatagt cttccataaa
     7921 cagatcgaag atgccttcgg tcttcatcca gtctgtcaga atcgaagtgg tcgtccgagt
     7981 agaccagggg ccggtacact ccagaacatc atcccatccc aacgcgttca taaatggaat
     8041 ggcgtcgcgg agatctcttc cactgccatg aggatgaaga tggctcttgc caacatgaca
     8101 ggtacgatac ccaagctgct tgaggggatg cagcaaagtc tcctcgacgt cccttagcct
     8161 tccaacattg tgccactggc c
//
LOCUS       DAOQUC010000008         8093 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_9837_length_8093_cov_3.020527, whole genome shotgun sequence.
ACCESSION   DAOQUC010000008 DAOQUC010000000
VERSION     DAOQUC010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8093
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9837_length_8093_cov_3.020527"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..402)
                     /locus_tag="PKH07_00280"
     CDS             complement(<1..402)
                     /locus_tag="PKH07_00280"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HNT33418.1"
                     /translation="MEIVWERDKCTEYRELFGQYLYGELPEDERERVESHLRTCPPCQ
                     QEIQEYQRVLDLLDNEARQEPATLPEGYLSARLRKAQIPRAVTPLRQRRRRLFYVAGM
                     AASFVMGVVAASFWTQSAPRVSPSALSSMFGT"
     gene            complement(408..989)
                     /locus_tag="PKH07_00285"
     CDS             complement(408..989)
                     /locus_tag="PKH07_00285"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNT33419.1"
                     /translation="MRTDAELIECAQKGDEQAFTELVDRYQNKVYGTAVRMLGDREEA
                     LEAAQEVFLRVWTHLATIRAGTNFGAWLYRITANHAVDLLRRRPALAPVELDEESTQV
                     IEMNFQDRTLTPRQVYAQQELKDRVQRAMQLLSPQQKAVFVLRHYQSLKLSEIADVLN
                     ISIGTVKATLHASLERLRVELSEKKSSVLREVR"
     gene            1180..1647
                     /locus_tag="PKH07_00290"
     CDS             1180..1647
                     /locus_tag="PKH07_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HNT33420.1"
                     /translation="MPQNHIRRAHVTEVTAIKSLIDRQVTKNLMLPRALNEIYQNLRD
                     FFVYFRDDRVVGCVALHIVWENLAEVRSLAVEEDYRGLGLGKYLVERAVEDARELSIP
                     RVFALTYVPDFFEKLGFRRIDKMELPHQVWADCVKCHKFPDCGEIPLVLDLDK"
     gene            1661..1972
                     /locus_tag="PKH07_00295"
     CDS             1661..1972
                     /locus_tag="PKH07_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HNT33421.1"
                     /translation="MYLARVVGNVVSTQKDPGLIGWKMLMCENLDMRTSPPKGTGSFS
                     VVVDLVGAGNGEIVIIVSGSSARMTDRTKTAPVDTAIVGIVDTLEVNGETIYCKKSTE
                     T"
     gene            1981..2322
                     /locus_tag="PKH07_00300"
     CDS             1981..2322
                     /locus_tag="PKH07_00300"
                     /inference="COORDINATES: protein motif:HMM:NF015289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HNT33422.1"
                     /translation="MDLARVIGCVVSTEKHKSLVGSKLCVIQPINENGEDVGAPIIAV
                     DIKSQVGSGETVYYVASGDAALLEEGKPMPVDAAVVGIVDTYETDPSLLKSVGSLRCY
                     RKYRSNKTESS"
     gene            2333..2620
                     /locus_tag="PKH07_00305"
     CDS             2333..2620
                     /locus_tag="PKH07_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020381430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HNT33423.1"
                     /translation="MNIGLVIGNVVSTIKHEAYENRTMLLVQPLDLDGKPKGLATIAV
                     DYVGAGTGDVVLLGAAPGLAPKVLNLKVCPVRELIMGIIDSTDVATGETSA"
     gene            complement(2637..2843)
                     /locus_tag="PKH07_00310"
     CDS             complement(2637..2843)
                     /locus_tag="PKH07_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015068236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YoeB family
                     toxin"
                     /protein_id="HNT33424.1"
                     /translation="MKQGYPTARRLSEILTKNSCQSPPPYERLLGDLERALSRRINIQ
                     HRLVYQAIDETKTVKVIRMWTRYD"
     gene            complement(2879..5140)
                     /locus_tag="PKH07_00315"
     CDS             complement(2879..5140)
                     /locus_tag="PKH07_00315"
                     /inference="COORDINATES: protein motif:HMM:NF022812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3160 domain-containing protein"
                     /protein_id="HNT33425.1"
                     /translation="MKHLLVLFLVMSLRFISRGGSSYAETTSPAEYSWEEIIREKDLS
                     DVQISALRRNGILVTDEAYKQIFTAYILLINPVFITSDSLLNAYHVLYEESVFRLESE
                     MASRLPDILRHIMDNIENVLQKCEGDPELLARAGRLARLVPGIALRLVDDSFHLKQED
                     LDAILEKEVEKIVQADGIGRPGWHSESDVSALRLDYSRHKPRGFYTRTEDLSRYFRAV
                     SWLQSIPFRIKKDEELAAVAMLSECLDSKTFTDPVKLSDTRSFFQAYRKMIGVCDDSD
                     LNFLFDMSQDFTRWDSNDYDFREWRDYIVKKAMQTNRRPLINDQIRFPPDDSSEAWEP
                     DVRIISAYRTPSAILFQRTTDIRRFMRDYPEGLEVAAALGSPFARNNIHGSQRSEILK
                     ETESCRAHFQGKSLYYDYLDALKTLLDPPDLDGPDFMKREAWQLKSCNTVLAGWAQLC
                     HTWILQSKQAVRYLSDAMLPVGFVEPEPDFFSKMADLAEKTRLFLRESNALKPDYTQV
                     LRALSNYRSGLLKEQSGLSEDYSVLSDEARMDTGIHYMLSEMNPVKRERGSDAYFQKS
                     VEWIDTLIADVKNGRVSDHPALESVCKEYNHDLNDLWIRFEKLSRRLEVIALKQLRGS
                     YLNDSDAAFIKSYGKELAEIMLYRDSARSHPRDDAPRIVDVFYNPLKKGYLQVGVCRA
                     RKLYVLYPWKGKRILCEGAIMPYYEFVSGTRLTDEEWRKILDSDQRPPIPKWMLPIVS
                     GRDLGKPAFSRRP"
     gene            complement(5213..5560)
                     /gene="mazF"
                     /locus_tag="PKH07_00320"
     CDS             complement(5213..5560)
                     /gene="mazF"
                     /locus_tag="PKH07_00320"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HNT33426.1"
                     /translation="MVAKREYVPERGDLVWITLNPQAGHEQSGRRPAMVLSPSAYNEK
                     VGLAIFCPVTSQVKGYPFEVPIPDDARVCGVILADQVKSLDWRVRKAELISRLPAPTV
                     DEVLKRTHVLMKR"
     gene            complement(5554..5796)
                     /locus_tag="PKH07_00325"
     CDS             complement(5554..5796)
                     /locus_tag="PKH07_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020384314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HNT33427.1"
                     /translation="METRVQKWGNSLALRIPKPFATEAGIEANSSVDVTLRGGKLIIT
                     PNAESEVSLSQLLARVNERNRHSEIDSGPAAGSEVW"
     gene            complement(6126..7358)
                     /locus_tag="PKH07_00330"
     CDS             complement(6126..7358)
                     /locus_tag="PKH07_00330"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HNT33428.1"
                     /translation="MTETTLYLIGIAFCLLCEAFFAGSEMGIVSLNKIRLKHLADEGN
                     RSALLILKMLEHPEKVLGTTLVAVNLAIVASASMASYVTALHFRQSTELLTTAIMLPL
                     IVVFGELIPKMIFRRHADRLTLLSVHGLRLAYFILFPMVTLCSGIGKGLAQVVGAGGE
                     EKNPYVSREELILLVQEQARKGTSKRQYEMAHQAFYFGETLARNIMVPLIEVKAVDAK
                     AGVAEIRRIVLECGFSHIPVYEERIDNIVGMIEMFDLLQARDTQTAREIMQEPVLVPD
                     TLSIATLLDTLRARRANIAILIDEYGGVSGIATTEDIMEEIVGDIRDEYDLEERSEII
                     REKHSLVVDGKVTIDRLNEDFGMKLPKEGVETVAGFLVSQFGALPSAGRKLAYRRLEF
                     EVLEASSRAIQRVRIRKR"
     gene            complement(7355..>8093)
                     /locus_tag="PKH07_00335"
     CDS             complement(7355..>8093)
                     /locus_tag="PKH07_00335"
                     /inference="COORDINATES: protein motif:HMM:NF015436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HNT33429.1"
                     /translation="KTAFRLGHHEGELDEREERILRRIIEFESKSVKSAMTPRGDIVA
                     CDVSKGLEGILQTFRENHFSRIPVYRDSFRTILGIAYIKDFLTQWEQAQRDFISLLRP
                     AFFVPEQQKIERLFLQLQRRKTSMALVVDEYGSLEGLVTLHDLLEEIVGDVPQRDEES
                     ECRVLGSNAWLVRAQMTATNFEDSFSIQMPDGDYDTIGGYLIGIRGELPQVGEHFELG
                     DLIITVHQREESRLNWLRVERKKVDLP"
BASE COUNT         1920 a         2202 c         2029 g         1942 t
ORIGIN      
        1 ggtaccgaac atcgaactca gggcgctcgg ggaaacacgt ggcgcagatt gcgtccagaa
       61 ggatgctgcg acaacaccca tcacaaaaga tgctgccatg cctgcaacgt agaacagtct
      121 tcgcctgcgc tgcctcagtg gagtgacagc ccttggaatc tgcgctttgc ggagtcgcgc
      181 acttagatac ccttccggca acgtcgcggg ttcctggcgc gcttcgttgt caagaagatc
      241 gagaacccgt tgatactcct gaatctcctg ctgacaaggg gggcacgtcc gcagatggga
      301 ttccacccgc tccctctcat cttcaggaag ttcaccgtag agatactgcc cgaacaattc
      361 tctgtattca gtgcatttat ctcgttccca gacgatctcc attctgatca ccttacctct
      421 ctaagaactg agcttttttt ttccgacaat tcgactcgga gtcgttccag tgacgcgtgc
      481 agcgtggctt tcacggttcc gatggaaatg ttcaggacgt cggcaatctc cgacagtttc
      541 agactctgat agtgccgcag cacaaagacg gccttctgtt ggggagaaag cagctgcatt
      601 gcccgctgga ctcgatcctt caactcctgt tgagcataga cctgccttgg ggtcaatgtg
      661 cgatcctgaa agttcatctc gatcacctga gtgctctcct cgtccaactc caccggtgcc
      721 agagcaggcc ggcgtcgcag caaatccacc gcatggttgg ccgtgatccg atagagccat
      781 gctccaaagt tggtacccgc tcgaatcgtc gccaagtgcg tccaaactcg aaggaacacc
      841 tcctgggctg cttccaaagc ctcttccctg tcccccaaca tgcgaaccgc cgtgccatag
      901 accttgttct gatatcggtc taccagttcg gtgaaggctt gttcatcgcc tttctgggca
      961 cattcgatca gttcagcgtc ggttcgcatg tggcctctct ttcatcctgc tgtatctaga
     1021 gacgcacggg tctgaaaaag gtttatcaga gtatcttgac gaaactttcc attgagcgta
     1081 gcctttttgt cagtttctta ctattgaaat tctcaagcct ttgagatatc aacaatgtga
     1141 cggttttcgt catatccatg tgaagaaggt gaaatagagg tgccgcagaa tcatattcgt
     1201 cgagcgcatg tcaccgaagt gactgccatc aaatcactta ttgaccgtca ggtgaccaaa
     1261 aacctgatgc ttccccgtgc cctgaatgag atctaccaga atctccgtga cttctttgtc
     1321 tatttccgag atgacagggt cgtcggttgc gtcgctttgc acatcgtatg ggaaaaccta
     1381 gcggaagtcc gctctctcgc ggtcgaggaa gactaccggg ggcttggctt gggcaagtat
     1441 cttgtggaac gtgccgttga ggatgcacgg gagctttcga tacccagggt ttttgcgttg
     1501 acctacgttc ctgacttttt tgaaaaactc ggattcagaa gaattgacaa aatggagttg
     1561 ccccatcagg tctgggcgga ctgcgtcaaa tgtcacaagt tccccgactg cggcgaaatc
     1621 cctctcgtct tggacctcga caagtaaagg agcaagtacc gtgtatctgg cgcgagttgt
     1681 tggcaatgtg gtctccactc agaaagaccc tggcctgatc gggtggaaaa tgctgatgtg
     1741 cgagaatctt gatatgcgca ccagtcctcc gaagggcaca ggcagtttct ccgtcgtggt
     1801 ggacttggtg ggtgctggaa acggggagat cgtcatcatc gtttcaggtt cttcggcacg
     1861 catgaccgat cgaacaaaga ctgctccggt ggacactgcc atcgtcggca tcgtggacac
     1921 actggaagtc aacggcgaaa cgatctactg caagaagagc acggaaacct agaggacaaa
     1981 atggatcttg ctcgtgtgat cggatgcgtt gtctcaaccg agaagcacaa gagccttgtc
     2041 ggaagcaaac tctgcgtcat ccaacccatc aatgaaaatg gagaagatgt gggcgcacca
     2101 atcattgccg ttgacatcaa gagccaggtg ggcagcggcg aaacggtcta ctacgtcgca
     2161 agtggtgatg ctgcacttct cgaagagggc aagccaatgc cggtggatgc tgcggtggtc
     2221 ggcatcgtgg acacgtatga aactgatccc agtctcctca aaagcgttgg atcattgcgt
     2281 tgctacagaa aatacagaag taacaagacg gaatcgtcct gaggatccct aagtgaacat
     2341 cggtttggtc atcggaaatg tcgtttcaac catcaagcat gaagcgtatg agaaccgcac
     2401 catgctgctt gttcaaccac tcgatctgga cggcaaacca aagggactcg ccacgatagc
     2461 ggtggactac gtcggtgctg gcacgggcga cgtcgttctg ctgggagccg cgccaggctt
     2521 ggcacccaaa gttcttaacc tgaaggtctg ccccgttcga gaactcatca tgggaatcat
     2581 tgacagcaca gacgttgcca ccggggaaac ttccgcctga ctgaccctgc acagccctaa
     2641 tcatagcgtg tccacatccg aatgactttc acggtcttcg tttcgtctat ggcctgatag
     2701 acgaggcgat gctggatgtt gatccggcga gaaagagccc tctcaaggtc tccaagcagc
     2761 ctctcatagg gcggaggaga ctggcaagaa ttctttgtca gtatctcgct cagtcgtcgc
     2821 gccgtaggat atccttgctt gatggatggc aaccagaatc gagaccgatg gcggtacctc
     2881 agggacgacg actaaatgca ggtttgccca agtctcttcc actcactatg ggcagcatcc
     2941 atttgggaat gggcggacgt tgatcagaat ccagaatctt cctccactct tcatcggtaa
     3001 gccgggtgcc tgaaacaaac tcgtagtacg gcataatcgc accctcacac aggattctct
     3061 tgcccttcca tggatagaga acatacagct tcctcgctcg acagacccca acttgaagat
     3121 agcccttctt cagaggattg tagaagacat caacgattcg tggggcgtca tccctcgggt
     3181 gcgagcgtgc ggaatcccgg tacagcatga tctcagcgag ttccttgcca taggacttga
     3241 tgaaggctgc gtcactatca ttcagatatg agcccctcaa ttgcttcaga gcaatcacct
     3301 caagtcttcg gctcaacttc tcaaagcgta tccacaagtc gttgagatca tggttatact
     3361 ctttgcacac agactcaagc gcaggatgat ctgagacacg accgtttttg acgtccgcta
     3421 tcagggtgtc aatccactcg acagacttct ggaagtatgc atccgaccct ctctcgcgct
     3481 tcaccgggtt catctccgac aacatgtaat gaattcccgt gtccatcctt gcctcatctg
     3541 acaagacaga atagtcttcc gataaaccgc tctgttcctt caatagcccg gaacggtaat
     3601 tcgagagagc tctgagcact tgcgtgtagt ccggttttag agcgttcgac tctctgagaa
     3661 agagccgggt cttctccgcc agatccgcca ttttcgagaa gaaatccggc tccggctcaa
     3721 caaaacccac gggaagcata gcatcactca agtaacgaac cgcctgcttt gactgcagga
     3781 tccatgtgtg acacagctgc gcccaacccg caaggacggt attgcagctc ttcagttgcc
     3841 atgcctctcg tttcatgaag tcaggcccat cgagatcggg gggatcgaga agggtcttga
     3901 gcgcatccag atagtcgtag tacagactct tgccttggaa atgtgctctg caggattctg
     3961 tctccttcag tatctcagac ctctgcgaac catggatgtt gttgcgtgcg aagggcgaac
     4021 ctagtgccgc ggcgacttcc agtccttctg gatagtctcg cataaagcgg cggatgtccg
     4081 tggttcgctg aaacagaatg gcgctcggag tgcgatatgc cgagatgatc cggacgtcgg
     4141 gttcccatgc ttcgctcgaa tcgtccggag gaaagcgtat ttgatcattg atcaaaggac
     4201 gcctattggt ttgcatcgcc tttttcacaa tgtagtcgcg ccactcgcga aaatcataat
     4261 cgttcgaatc ccaacgggtg aaatcttgtg acatatcgaa aaggaagttc agatctgagt
     4321 catcacagac gccaatcatt ttgcgatagg cttggaaaaa cgatctcgtg tcactcaatt
     4381 tgacaggatc cgtgaatgtt ttggagtcga gacactcgga aagcattgca actgccgcca
     4441 gttcctcgtc tttcttgatg cgaaacggaa tagactgcag ccacgagacc gcgcggaaat
     4501 aacgactgag atcctccgtg cgtgtgtaga aaccgcgagg tttatggcgg ctgtaatcta
     4561 gcctgagggc agacacatcc gactcgctgt gccatccagg ccgaccaatg ccatcagcct
     4621 ggacgatctt ctcaacttcc ttttccagaa ttgcatccag gtcttcttgc ttaaggtgaa
     4681 aagagtcatc aacgagcctc agagctatcc ctggaacgag cctcgcaagt ctgccagctc
     4741 tcgccagaag ttccggatcc ccctcacatt tctgaaggac gttctcgatg ttatccatga
     4801 tgtgccgaag gatatccggt agtcgtgaag ccatctcact ctcaagccga aagacagatt
     4861 cctcatagag cacatgatac gcattgagca aggagtccga ggtgatgaaa accgggttaa
     4921 tgaggagaat atacgctgta aatatctgtt tgtatgcctc atctgttacg agaatcccat
     4981 tccttctcaa tgctgagatt tggacatcag aaagatcttt ctcacggatg atctcttccc
     5041 aagagtattc ggcaggagag gttgtttcgg cgtaggacga accacccctc gagatgaagc
     5101 gcagcgacat aaccaagaac agaaccagaa gatgtttcat ctgtcaccac cccagtctgg
     5161 ttgatgcatg aagagacaca gttatctcag tgcagagtat agcgaggctt gtttatcttt
     5221 tcatcaaaac gtgcgttctc ttcagtacct catcaactgt tggagctggc aaacgggaaa
     5281 tcaactccgc tttccgaacc cgccagtcca gactcttgac ctgatccgcc aggatgacgc
     5341 cgcagactct cgcatcatcg ggtatcggaa cctcgaacgg atagcctttc acctgacttg
     5401 tcaccgggca gaagatcgcc aaccctacct tctcgttgta ggctgaaggt gaaagcacca
     5461 tcgcaggtct tcgaccactc tgttcatgac ccgcttgagg attgagcgta atccagacca
     5521 aatcaccacg ttcaggaaca tattccctct tggctaccat acttcactcc ccgctgccgg
     5581 accgctgtct atctcgctgt gcctattccg ttcgttgacc cgcgccagca gttgtgacag
     5641 agatacttca ctctccgcat tgggcgtgat gatcaacttc cctcctctca atgtgacatc
     5701 cacagatgaa ttggcttcta tccccgcttc ggtagcaaag ggtttcggaa ttcgaagagc
     5761 aagactgttt ccccatttct gcacgcgggt ttccatcttc aatacctcca ctctgtatct
     5821 ataaagaaga tacatcagtt tcagtacttg gtcaacaaat atgccgttcc cttatgctgt
     5881 tctccggaaa ggtgccccga ttgcttcaga caacgcaagg cgttaccctc cccttcacgt
     5941 atgcagtacg gccagacgtt gttgtgtcga gtccggttca tggactcgat cggtggaaat
     6001 agccgtggaa tcgaggcagc accgcgttcc tgagagcagc gtacactgga ttgtgagact
     6061 gatttcgact gcgcgggcaa cagattatgc ctactgtcct gtgcggtgct tgcttgcgag
     6121 agaaactacc tctttcggat tcgaactcgt tgaattgcgc gtgaagaggc ttcaagaact
     6181 tcaaactcca gtctcctgta tgccagtttc cggcctgcag aaggaagggc accgaactga
     6241 gagacaagaa atccggcaac cgtctcaacc ccttccttgg gaagtttcat gccaaagtct
     6301 tcattaagtc gatcgatggt gacttttccg tccactacca aagagtgttt ctcacggatg
     6361 atctcgctgc gctcttcaag atcgtattcg tcacggatgt ctcctacgat ttcctccatg
     6421 atgtcttcgg tggtcgcgat gccggaaacc ccaccatact catctatcaa aatggcaatg
     6481 ttggcgcgtc tggctctgag cgtatccagt agcgtggcga tggacagtgt atcgggcacc
     6541 agcacaggct cctgcatgat ttcccgggcc gtctgagtgt cgcgggcttg caggagatcg
     6601 aacatttcga tcatgcccac aatgttatca atacgttcct catacacagg gatatgcgag
     6661 aatccgcact cgagaacaat ccgacggatc tctgcaacgc ccgcctttgc atccactgcc
     6721 ttgacctcaa taagcggcac cataatgttc ctggccagcg tctcaccgaa atagaacgcc
     6781 tgatgcgcca tctcatactg acgtttcgag gtaccctttc gagcttgctc ctggacaagg
     6841 agtatgagtt cctcacgaga gacgtaagga ttcttctcct ctccgccagc gcccaccacc
     6901 tgcgcaaggc ctttgcctat accactgcag agagtcacca tcggaaacag gatgaaatac
     6961 gcaagacgaa gaccgtgaac cgaaagcagg gtcagccgat ctgcatggcg acgaaagatc
     7021 atcttgggaa tcagttctcc aaaaacaacg ataagaggca gcatgatcgc tgttgtgagc
     7081 agttctgtgg attggcggaa atgaagtgcg gtgacgtaac ttgccatgga tgcggacgcc
     7141 acaatggcca gattcacggc gacaagcgtc gttccaagaa ccttctccgg atgctccagc
     7201 attttcaaga tcagaagtgc acttctgttt ccctcgtccg ccaggtgctt gagacggatc
     7261 ttgttcaagg agacgatacc catctccgaa ccggcgaaga aggcttcaca aagcagacag
     7321 aaggcgatgc cgatgagata gagggtggtt tctgtcatgg gaggtccacc ttctttctct
     7381 cgacccggag ccagttcaag cggctttctt ccctctgatg caccgtgata atgagatcac
     7441 cgagttcaaa gtgttcgccg acctgtggaa gctctccccg tatgccgatg aggtatccgc
     7501 cgatggtgtc atagtcgccg tccggcatct ggatcgaaaa ggagtcctcg aaattcgttg
     7561 cagtcatttg tgctctcacg agccaggcat tgcttcccag aacacggcac tctgactctt
     7621 catcgcgttg gggaacatcg ccaacgatct cctccagaag gtcatggagt gtgaccaagc
     7681 cctcgagact tccgtattcg tctacaacca gagccatgga agtcttgcgg cgctgaagtt
     7741 ggagaaagag gcgctcaatc ttttgttgtt ccggaacaaa gaaggcagga cgcagcagac
     7801 tgatgaagtc ccgttgcgcc tgttcccact gcgtcagaaa atccttgata taggcgatgc
     7861 ccagaatggt gcgaaaactg tcccggtaga ccggaattct gctgaaatga ttctcgcgaa
     7921 acgtttgcag aattccttcc agacctttgg atacatcaca ggcaacaata tcccctcgtg
     7981 gtgtcatggc ggacttgacg ctcttggact cgaattcaat gatacgcctc agaatccgct
     8041 cttcgcgttc atcgagttcc ccctcatggt gtccaagccg gaacgcagtc ttt
//
LOCUS       DAOQUC010000009         8048 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_9906_length_8048_cov_3.454523, whole genome shotgun sequence.
ACCESSION   DAOQUC010000009 DAOQUC010000000
VERSION     DAOQUC010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8048
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9906_length_8048_cov_3.454523"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..301)
                     /locus_tag="PKH07_00340"
     CDS             complement(<1..301)
                     /locus_tag="PKH07_00340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33430.1"
                     /translation="MSPELKTKDIGSLRGRWKIKLLLIVVFFLICGVVSAGVFLYQKI
                     GTERAQKDYLLAQTLLDSGRAKDAREAMLPIYQKYPTFPQREKVIYSLAKSFQDAE"
     gene            complement(291..449)
                     /locus_tag="PKH07_00345"
     CDS             complement(291..449)
                     /locus_tag="PKH07_00345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33431.1"
                     /translation="MEQASACKCVGYAHSFEALVRERNLTLWSLVSWEIYGERLSPNG
                     GTFTGNVT"
     gene            complement(498..3287)
                     /gene="secD"
                     /locus_tag="PKH07_00350"
     CDS             complement(498..3287)
                     /gene="secD"
                     /locus_tag="PKH07_00350"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HNT33432.1"
                     /translation="MPTFQVWKPIVVLVVLIAAVFAVYPSVRWYSLSPEDRINQQSTE
                     EMREVEARIEQMKSENRQDEIPAARSEYDELKAKFGQLRRRAIPLGLDLQGGIHAVLQ
                     VEIEQLLEDRRAEAVDRSLEIIRNRIDQLGVLEPVLQRQGANRIIVEVPGITDPTRII
                     DLLGQTARLEFRMIKEAADVYGHIREIDRKLGVGLSEKVELSEDGGELEVREADQKYV
                     DQVIMRETARAVIPVGYELLWSRPETSSTKGSVYRLYEVKEKAELTGEQLKDARMEID
                     QARFNEPYVSIELDRRGGQIFRQVTGDNVGKRLAIVLDDRVYSAPVIRERIPHGMARI
                     TGRFTIQEARDLGIVLRAGSLPADISVVETRAVGPSLGMDSIRQGIRAGLGGAVVVLG
                     FMIVYYVVAGAIADFAVILNIFVLLGAMALFKTTLTLPGIAGIVLTVGMAVDANVLIY
                     ERIREELRVRRDKALAISVEKGYSRAFLTILDANLTTLLTAVMLFAFGTGPIRGFAVT
                     LSLGIVISMFTAIFVTRIIFDFLTNQVNVKELKIGFFQIFGNTRIDFMRIHKLAIGVS
                     LLFILVGLGTMFAKGGLKTNIDFSGGNLVQVRFNEPKETGEIRNVLQSSGYGHADIQR
                     VLDDANEFLVRVKEDARSPEGRDELRARFEKAAKDAFSDKNPTVQLTKGIEQVEPKPG
                     QEARDEYQIALSAPVTEQEIFDAMRNAGFPVADAVPVADQENTYAIKVSQGVTEQLLA
                     VLGKGFGEDAFELRRQEKVGAKVGTELQKKAINAALFSIIGILAYVAWRFQFGFALGA
                     VLALVHDVLVTLGIFAITGREISLSVVAAILTLIGYSLNDTIVVYDRIRENQGLLGGP
                     KDMPTVINNSINQTLSRTILTAATTLFVVIALFMFGGIVLNDFAFALLVGIVVGTYSS
                     IYIASPILIYWQSRR"
     gene            3543..4493
                     /locus_tag="PKH07_00355"
     CDS             3543..4493
                     /locus_tag="PKH07_00355"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNT33433.1"
                     /translation="MPESYEIRAFKAEDTPRLLQVWNRSLPIDGVGLSVFEQKVILDD
                     NFSPEGLLVAETKDGVQGFIYGVVRRVPMPGLGLQEDKGWITAWGVVPEARGQGMGKA
                     LLNASLGFFKQENRKQVIFSTYSPNYFVPGVDMKAYAPAMQLLGKAGFEKVTEFLSMD
                     ARIVQYVRPDEVVAKEEKLRQSGIDVRFYERADLFNYLEFQRKHMPPDWLMLAKNNLR
                     DLCRGMFEPDQIVIALDQGTIVGFCQHEAEHFGPFGVADSHQGKGIGTVLLSKVLERM
                     HQKGLHGAWLLWTGDRAAQGVYGRLGFTETRRFAMMCKNI"
     gene            4509..5252
                     /locus_tag="PKH07_00360"
     CDS             4509..5252
                     /locus_tag="PKH07_00360"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNT33434.1"
                     /translation="MDSWYKGMYGEHYYRASFAAMPAERTQKEVEFIRTVLNLPPGAR
                     VLDLCCGPGRHTLALAQLGYRMSGLDLSEHYIKLARNHAKRENLDVDFQIGDMRRIPF
                     RSKFDAVINIFTSFGYFDSDEENASVIQGVVGKLKPGGKFLLDVINRDYIVRNMTQSH
                     WLDRGDILVLQDHSFDPVTSRTEDRWVFLEKKSRKEFDVSIRLYALHEIIALFQRAGL
                     TYQKAWGNFAGEPFDYTSQRIIALGVKPQ"
     gene            5296..6330
                     /locus_tag="PKH07_00365"
     CDS             5296..6330
                     /locus_tag="PKH07_00365"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNT33435.1"
                     /translation="MRVDEELVVIIGHCLTLDIQAEEIYQALSQRFEEPEMRSYWQTM
                     ALDEQEHVEYWKELLELARKGMVPQVFDKTYEIRKQLESLILKNRDVVGQCATINNAV
                     DAFRIAYQLEFNILHPAFSSLFHFVRIGAGESSPWDSYEKHLSSLSNHLARFGAMTPD
                     LDFIGEMVSSLWNRNCDLVLQAHIDPLTEVHNRRGFFHVVIPLAHLAQRMHYNVAIMM
                     VDIDHFRTVNEQHGHLTGDSLLTLIAHCVKSQVRRSDAVGRYGGEEFIVFLINAEKEY
                     LGEISERMRQHVEDITRDRIPVTVSIGISYDNLEGDVEEQIDRLVKEADDYLSQAKKA
                     GRNKVVMKQG"
     gene            complement(6403..6660)
                     /locus_tag="PKH07_00370"
     CDS             complement(6403..6660)
                     /locus_tag="PKH07_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019508086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HNT33436.1"
                     /translation="MKYCIEFAKSAERQLGKLPTSIQIRLVKKIESLGSDPRPQRVEK
                     LIGREHGYRIRVGDYRVLYRVEDDVLTVLVVAVGHRREVYR"
     gene            complement(6667..6924)
                     /locus_tag="PKH07_00375"
     CDS             complement(6667..6924)
                     /locus_tag="PKH07_00375"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HNT33437.1"
                     /translation="MNRLAANEVRRDFAEALNQVAYKGDRIVIHRRGKDVAVLVPIED
                     LDLLKRAAEEQDLREFDEGLKSAETEGTVSLKAIKKRLRME"
     gene            7210..8040
                     /locus_tag="PKH07_00380"
     CDS             7210..8040
                     /locus_tag="PKH07_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012071210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005634 - nucleus [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HNT33438.1"
                     /translation="MSKKIEVLSRQVRLASVNEQDYVCLTDIARYKDPERTDYLIFNW
                     LRNRNTIEFLGIWEQINNPVFNPIEFDGIRKEAGLNSFILTAKQWIERTGAIGIQSKA
                     GRYGGTYAHLDIALEFASWISVEFKLYLIKEFQRLKEQERQTLGWDIRRNLARINYRI
                     HTDAIQTHLIPPELEKAQINSVYASEADVLNMALFGKTAAQWRSLNPGEKGNIRDQAT
                     SAQLVCLSNLENLNALFIGQGLSQSERLTRLNQIAIQQMKILAAEDRLPEPTQGRRSK
                     "
BASE COUNT         1992 a         2152 c         2108 g         1796 t
ORIGIN      
        1 cttcagcatc ctgaaaactc ttggccagag agtaaatgac cttctcccgc tgaggaaacg
       61 ttggatactt ctggtagatg gggagcatgg cctcacgggc atctttcgcc cggccactgt
      121 ccagaagcgt ctgtgccaga agatagtctt tctgagcgcg ttcagtaccg atcttttggt
      181 aaaggaatac acccgcagat acgacaccac agatgaggaa gaagacaact atgagaagta
      241 acttgatctt ccatctgcct cttaggctgc ctatgtcctt ggtcttcagc tcaggtgaca
      301 ttgcccgtaa acgttcctcc atttggagag agacgctctc catatatctc ccaggaaacc
      361 agactccaga gggtaaggtt tctttctcga acaagcgcct caaaggagtg agcatagccc
      421 acacacttgc aggcgctggc ctgctccaag gaatctccag ataagacgta tccatcggca
      481 tcggaattca gaccgattta ccttctggac tgccagtaaa taagaatcgg gctggcaata
      541 taaatcgagg aataagttcc cacgacaatt cctaccagaa gcgcaaaagc aaaatcgttc
      601 aagacgatgc caccaaacat gaacagcgca atcaccacga agagcgtggt tgccgccgtc
      661 aggatggttc gcgaaagagt ctggttgata ctgttgttga taaccgtagg catatccttg
      721 ggaccgccca ggagtccctg attctctcgg attcggtcat agacaacgat ggtgtcattc
      781 agagagtatc caatcagggt cagtatggcg gcgacgacac tcagactgat ctcacgaccc
      841 gtgatggcga agatgcccag agtgaccagg acgtcatgca ccaaagccag cacggctccc
      901 aaggcaaatc caaattggaa gcgccacgca acataggcaa gaatcccaat gatggaaaac
      961 aatgccgcgt tgattgcctt cttctgcaac tcggttccaa ctttggcgcc aactttctcc
     1021 tgtcgtcgca gttcgaaagc gtcctcgcca aaacctttgc ctagaactgc gaggagttgc
     1081 tccgttaccc cctggctgac cttgatagca taggtgtttt cctgatctgc gacgggaaca
     1141 gcatcagcaa ccgggaatcc tgcatttcgc atcgcatcga aaatctcctg ttcggtgaca
     1201 ggcgcagaca aagcaatctg gtattcatca cgtgcttcct gtccgggctt cggctccacc
     1261 tgttcaatgc ctttggtcaa ttgaacagtt gggttcttgt ccgagaacgc atccttggct
     1321 gctttttcaa aacgagcgcg cagttcatcg cgtccttcgg gagagcgtgc atcttccttg
     1381 acgcggacaa ggaattcatt ggcgtcgtcc agaactctct gaatgtccgc gtgtccgtaa
     1441 ccactcgact gcaagacatt gcgaatttca ccggtttctt tgggctcgtt gaaacgcacc
     1501 tgcaccaggt taccaccgga gaaatcaatg ttggttttca gaccgccctt ggcgaacatg
     1561 gtgcccaagc caacaagaat gaacagcagc gagacaccaa ttgccagttt gtgaatgcgc
     1621 atgaagtcga tccttgtgtt gccgaagatt tgaaagaatc caatcttcaa ttccttgaca
     1681 tttacctggt tggtcaggaa atcaaagatg atgcgggtga cgaaaatcgc cgtaaacatc
     1741 gaaatgacga tacccaaact cagggtcact gcgaaaccac gaatggggcc tgttccaaac
     1801 gcgaacaaca tcacagcagt taagagtgtt gtgagattgg cgtccaaaat ggttagaaac
     1861 gctctggaat agcccttttc caccgagatg gcgagtgcct tgtcgcgacg aactcggagt
     1921 tcctcgcgta tacgttcgta gatgagcacg ttggcatcaa cagccatacc aacagtcaac
     1981 acaataccgg ctataccggg aagagttaga gtggtcttga agagcgccat agctcccagc
     2041 aacacgaaga tgttgagaat gacggcaaag tctgcaatcg cacctgcgac cacatagtag
     2101 acaatcataa agcccagaac gacgaccgca ccccccaagc ccgcacggat gccctgtcgg
     2161 atggagtcca tacccaacga cgggccgacc gcgcgtgtct ccacaacgct gatgtccgcg
     2221 ggaagagatc cagctcggag cacaatgcca aggtctctgg cttcctgaat ggtgaatcgc
     2281 cctgtgattc gagccatgcc gtgaggaatc cgctcacgaa tgaccggtgc ggagtacacg
     2341 cggtcgtcca gaacgattgc aaggcgcttg ccgacgttgt ctcctgtgac ctggcggaat
     2401 atctggccgc cgcgtctgtc gagttcgata gagacataag gctcattgaa acgagcctga
     2461 tcaatttcca ttcgggcgtc tttcagttgt tcgcccgtca gttccgcctt ttccttgact
     2521 tcatagaggc gatagacgct gcccttggtg ctgctggtct cgggtcggct ccacagcagt
     2581 tcgtatccga cagggatgac ggcacgcgcc gtttcacgca tgataacctg gtctacgtat
     2641 ttctgatcag cttcccgaac ttccagttct ccgccgtctt cgcttagttc gaccttctcg
     2701 ctcagcccaa cacccagttt acggtcaatc tctcgaatgt ggccgtatac atccgccgct
     2761 tccttgatca ttcggaactc aaggcgtgct gtctgaccca ggagatcaat gatgcgggtc
     2821 ggatcagtga tgcctggaac ttcgacaatg atccgattag cgccttgacg ttgaagcacc
     2881 ggctccagaa cgccgagttg gtcaattcgg ttgcggatga tctccagtga acgatccacc
     2941 gcttcggctc gccggtcttc gagaagctgt tcaatttcca cttgaagaac ggcatggatg
     3001 cctccctgca aatccagtcc gagcggaatg gcacgacgac gcaattggcc aaatttcgcc
     3061 ttcaattcat catattccga ccgcgccgcg ggaatctcat cctggcgatt ctccgacttc
     3121 atctgttcaa tgcgggcttc cacctcacgc atctcttcgg tgctttgctg gttgatacga
     3181 tcttcggggg aaagggaata ccatcgcacc gaaggataga cagcgaacac cgcagcgatc
     3241 agaaccacga gcacaacaat cggcttccaa acctgaaaag tgggcatctt cgaagagtcc
     3301 tcccaatcaa aaaatcatga ctcaaatata ggaacgtact gcacaaagcc tgggcacacc
     3361 gacacacgac ccgcagttgc cgctggactc gacaatactc catattaaac gacggagtat
     3421 agatttgcat ttcccgactt gtcaatgcca tttgacagac aggccaggat aagtataaga
     3481 atcagggtct agaagcggaa accagaatgc aggagtcttg caccgctgaa gggagaatac
     3541 cgatgccgga atcttatgaa attcgcgcgt tcaaggcgga ggacacccca cggctgcttc
     3601 aagtgtggaa ccggtcgctg cccattgatg gggttggatt gtccgtgttt gagcagaagg
     3661 tcatcctgga tgacaacttc tcaccggagg ggcttttggt agcggagacc aaagatgggg
     3721 ttcaagggtt catctatggt gttgtgcgcc gagtcccgat gcctggtctt ggtcttcagg
     3781 aagacaaggg atggatcacg gcgtggggtg tggtgcccga ggcccgcgga cagggaatgg
     3841 gcaaagccct gctgaatgcg tctctcggtt ttttcaagca ggagaatcga aaacaggtca
     3901 tcttctcgac ttactccccg aattactttg ttccaggagt ggacatgaag gcatacgctc
     3961 cagcgatgca actgctgggg aaggccggat tcgaaaaggt aaccgagttt ctcagcatgg
     4021 atgcccgaat tgtccaatat gtgcgacccg atgaggtcgt tgccaaggaa gagaagctca
     4081 ggcagagtgg cattgacgtg cgattctacg aaagagcaga tctcttcaat tacctggagt
     4141 ttcaaagaaa gcacatgccg ccagactggc tgatgctggc aaagaacaat cttcgagacc
     4201 tgtgccgcgg gatgttcgag cctgaccaga tcgtcattgc cttggatcag ggaacgatcg
     4261 tcggattctg tcagcatgaa gcagagcatt ttggcccctt tggtgttgcc gactcgcatc
     4321 agggcaaggg catcggaacg gtgcttctgt ccaaagtgct ggaacgaatg caccagaagg
     4381 gacttcacgg agcgtggctc ctgtggacag gcgaccgagc tgcccagggt gtttatggac
     4441 gtcttgggtt cacagagaca cgccgatttg ccatgatgtg caagaacatc tgaggagcga
     4501 gacacagcgt ggacagttgg tacaagggca tgtacgggga gcactattat cgggcaagct
     4561 tcgccgccat gcctgcagag cgtacgcaga aagaagttga gttcatccgc acggtactga
     4621 atttgccgcc gggcgcacgc gtgcttgatc tttgctgcgg tcctggacgg cacacgcttg
     4681 ctctcgcaca actcgggtac cggatgtcgg gactggatct ttcagagcat tacatcaaac
     4741 tggcaagaaa ccatgccaag cgggagaatc tggatgttga tttccagatt ggcgacatgc
     4801 gacgaatccc tttccgatcg aagttcgacg cggtcatcaa catcttcacc agctttggat
     4861 acttcgacag cgatgaggag aatgcctcgg tcattcaagg tgttgtgggc aagctcaagc
     4921 caggcgggaa atttctgttg gacgtgatca accgggatta tatcgtgcgc aatatgacgc
     4981 aatcacactg gctggatcgg ggagatatcc ttgttctgca ggatcactct tttgatccgg
     5041 tgaccagccg aacggaagat cgctgggtct ttctggagaa gaagagtcgc aaggaattcg
     5101 acgtatctat aagattgtat gctctgcatg agattatcgc gctgtttcag agagcgggac
     5161 tcacctatca gaaagcctgg ggcaactttg ccggagaacc gtttgattat acatctcagc
     5221 gaatcattgc cttgggagtg aaaccgcagt agcagcgcct gagcgcggaa gcgatggagc
     5281 aagagggaga caatcatgag agttgacgaa gagctggtcg tgatcatcgg acattgcctg
     5341 acgttggaca ttcaagcaga ggaaatctat caggctctct cacaacggtt cgaggaaccg
     5401 gaaatgcgct cttactggca gaccatggct cttgacgagc aggagcatgt ggaatactgg
     5461 aaggaactgc tggagctggc gcgcaagggc atggttccac aagttttcga caagacctat
     5521 gagattcgca agcagctgga gtctctcatt ttgaagaacc gggatgtggt cgggcagtgc
     5581 gctacgatca acaatgcggt ggatgctttt cggatcgcgt accagctgga gttcaacatc
     5641 cttcatccgg cattcagttc cctgtttcat tttgtccgga ttggagcggg cgagagttct
     5701 ccctgggact cctatgagaa gcacctgtcg tctctttcca accatctcgc gcgatttggt
     5761 gcaatgacgc ctgacctgga cttcatcgga gaaatggttt caagtctctg gaatcgcaac
     5821 tgcgatctcg tgctgcaagc ccacattgat cctttgactg aagtgcacaa ccgacgcggt
     5881 ttcttccacg ttgtgatccc gttggcgcat cttgcgcagc ggatgcacta caacgtggcg
     5941 atcatgatgg tagacattga ccacttccgg acagtcaacg aacagcacgg tcatctgacc
     6001 ggagattcgc tgttgacgct cattgcgcac tgtgtcaagt cgcaggttcg gcgttcggac
     6061 gccgtcggtc gctacggtgg tgaggaattc atcgtctttc tgatcaatgc cgagaaggaa
     6121 tatctgggtg aaatcagtga acgaatgcgt caacatgtcg aggatatcac ccgcgaccgg
     6181 attccagtca ccgtcagcat cggcatttcc tacgacaacc tcgaaggaga cgtcgaagaa
     6241 cagatagacc gtttggtcaa agaagcggac gactatctga gtcaggccaa aaaggcagga
     6301 cggaacaagg ttgtcatgaa acaaggctga acgcgggcga ttccatccga tattacagtt
     6361 cgcaacaggc gcttctgcgc gtcggcatcc atcaccaaga gttcaacgat acacttctct
     6421 gcgatgtccg actgccacga ccaggaccgt caacacatca tcttccaccc gatacaaaac
     6481 gcggtagtca ccaacacgaa tacgatagcc gtgctctctg ccaatgagtt tttcaaccct
     6541 ctgcgggcgg ggatcactac ccagtgattc tatcttcttg acaagacgga tctggataga
     6601 ggtgggaagc ttgcccaact gtcgctccgc acttttggcg aattcaatac agtatttcat
     6661 gcagagctac tccatccgca gtctcttctt aatcgctttt agggaaaccg tgccctcagt
     6721 ctccgccgac ttcaggccct cgtcgaattc gcgcagatct tgctcctctg ctgctcgctt
     6781 gagcaaatcc aaatcctcaa tgggaacaag aactgcaaca tccttcccac ggcgatgaat
     6841 gacgattcgg tcgcccttgt aggcaacctg gttgagcgct tcggcgaaat ctctcctcac
     6901 ttcattggct gccagtctgt tcatttctca atcactcctc atgtacataa cgttcttcag
     6961 gtactatatg tacatgatgt gtttttgtca acatcgagac ggaacaagaa atggacagtc
     7021 tcacagagaa caacggtaac tgcctggtca ggaagcgcac ggtctgcggt tgatttgctt
     7081 tcgagagagg gtgtatgctg aagatgtggt ggtggttcgc cgcagagaag agacagatcc
     7141 cgtatcctat taggagcatt cgaaatgaag gagcgattag ccaagatgag gctgaaggga
     7201 gattgaccga tgagcaagaa gatcgaagtc ctttcccgcc aagtccgtct tgccagcgtc
     7261 aatgagcagg actatgtctg tctgacggac atcgcccgtt acaaagatcc agagcgcacg
     7321 gactacctga tattcaactg gttgcgcaac cgcaacacca ttgagttcct tggcatctgg
     7381 gagcaaatta acaaccccgt ttttaatccc atcgaattcg atgggattag aaaagaagcg
     7441 ggtctgaaca gcttcatcct gactgccaag caatggatcg agcgcaccgg agccatcggc
     7501 atccagtcca aagccgggcg ctatggcggc acctatgcgc acctggacat cgccttggag
     7561 ttcgcctcgt ggatttctgt cgagttcaaa ctctacctca tcaaggagtt ccagcgcctc
     7621 aaggaacagg aacgccagac gcttggttgg gacatccgcc gaaaccttgc ccgcatcaac
     7681 tatcgcatcc acaccgacgc catccagacc cacctcatcc cgccggagtt ggagaaagcc
     7741 cagatcaact ctgtctatgc cagcgaagca gacgtgctta acatggcgct cttcggcaaa
     7801 accgccgccc aatggcgtag cctcaacccg ggggaaaagg gcaacatacg cgatcaagcc
     7861 accagcgccc aacttgtctg cctctcaaac ctcgaaaacc tcaacgccct gttcatcgga
     7921 cagggattgt cccagtccga acgcctcacc cgtctcaacc agatcgccat ccagcagatg
     7981 aaaatcctcg ccgccgagga ccgcctgcct gaaccgacac aggggaggag aagcaaatga
     8041 agaagggt
//
LOCUS       DAOQUC010000010         7842 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_10232_length_7842_cov_3.392192, whole genome shotgun sequence.
ACCESSION   DAOQUC010000010 DAOQUC010000000
VERSION     DAOQUC010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7842
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10232_length_7842_cov_3.392192"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1602)
                     /locus_tag="PKH07_00385"
     CDS             complement(<1..1602)
                     /locus_tag="PKH07_00385"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007290317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HNT33439.1"
                     /translation="MEKTIFVTGGVCSSLGKGVAAASLGCLLESSGLRVTLQKIDPYL
                     NVDPGTMNPYQHGEVYVTDDGAETDLDLGYYERFTSAPLSRDNSVTTGRVYQAVIERE
                     RHGEYLGQTVQVIPHITNEIKSRILTVARNTDADVQLCEIGGTVGDIESVPFLEAIRQ
                     YGQERGRENVIYIHLTLIPYIGVAGEMKTKPTQHSVKELREIGIMPDILLCRTRSPLP
                     KDMKEKIALFCNVAPRAVISAVDIQSTIYEIPSWLRQEGLDRIVVEKLGLKAHEHDWS
                     PWQKIVDTIKSAPRCARIAVIGKYMELQDAYRSLFEAIIHGGIGNDSRVEIVKFDAED
                     LEQRPAGEILADVDGILVPGGFGMRGIEGKIQAIRYARENRIPFFGICLGLQCCVIEF
                     TRNACGLAGANSTEFDPKTSHPVVSLLAEQRDIVDMGGTLRLGAWPCTVKPSSRAQSA
                     YGQDEIMERHRHRYEVNNAYIEQMQEHGMIVSGICPQGNLVEMVEIPDHPWFVACQFH
                     PEFKSRPRNPHPLFRDFVRAALEYQT"
     gene            complement(1784..2533)
                     /gene="kdsB"
                     /locus_tag="PKH07_00390"
     CDS             complement(1784..2533)
                     /gene="kdsB"
                     /locus_tag="PKH07_00390"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015346754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HNT33440.1"
                     /translation="MRSICVIPARFGSQRLPGKPLVEICGKPLIQHVWERVRMAHRVN
                     AVLVATDDQRIADVVHAFGGQAVMTSPDCASGSDRVGEVIAERDEQIVINVQGDEPLI
                     DPRAIERLIEEFKLDPNLEMATLARPLEIRDVANPNAVKVVCDNDDFALYFSRSPIPY
                     WRDAGKPGQKMPEYWHHVGVYAYRREVLLSFLSLPEGRLEKAEKLEQLRALENGIWIK
                     VLKGNWPCLGVDTPEDIKKVEALLAKETDGD"
     gene            complement(2608..3618)
                     /gene="rfaE1"
                     /locus_tag="PKH07_00395"
     CDS             complement(2608..3618)
                     /gene="rfaE1"
                     /locus_tag="PKH07_00395"
                     /inference="COORDINATES: protein motif:HMM:TIGR02198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase"
                     /protein_id="HNT33441.1"
                     /translation="MDRSRMMQRKMLGSLKGLHGTGVLVVGDLMLDRFIWGKVSRISP
                     EAPVPVVDVQSETYVPGGAANVMTNLHALGAEVHVAGTVGQDREGRILLDLLSENGLN
                     TQGIFPIKGKPTTLKTRIIAHSQQLVRVDREVRESVSASLTTKLLDSIERCLPRVKAI
                     VIADYAKGVVSRDLIEGIVSAASKRHAGAIPVLVDPKTLDFSVYRGVDLIKPNQQEAA
                     QAAHILISSHEDLRLAAERLLRDVECQAVLITRGEEGMSLFEKEHGFTHIPTTARQVY
                     DVTGAGDTVMAVLALCLASGVDLVTAAHLSNHAAGVVVGKVGVSTVSLHELQEAITKD
                     DP"
     gene            complement(3903..4862)
                     /locus_tag="PKH07_00400"
     CDS             complement(3903..4862)
                     /locus_tag="PKH07_00400"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013703180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HNT33442.1"
                     /translation="MRRALDFERPLVELQSKIEEMTRYASERGIDLSTEISAMKEKLQ
                     EVEKSIFSQLTPWQRIQLARHPDRPYFLDYITRMTTDFFELHGDRCYRDDAAIVGGLA
                     CLDGRPIVALGHQKGRDTKENLKRNFGMAHPEGFRKALRLMQLAERFGLPVLTFIDSS
                     GAYPGVGAEERGQALIIAENLLGMAALNTPIIVVVTGEGGSGGALAIGVGDRVIMLEH
                     AYYAVCTPEACAAIIWKDRTKAAEAVESMKILSGDLHRLGIIDEILPEPMGAAHRDYD
                     RMAETIKQAVLRNLAELDQIPKEDLSRKRYEKFRRMGIFEKTS"
     gene            complement(4953..>7842)
                     /gene="dnaE"
                     /locus_tag="PKH07_00405"
     CDS             complement(4953..>7842)
                     /gene="dnaE"
                     /locus_tag="PKH07_00405"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004226.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HNT33443.1"
                     /translation="VATNDCHYLLRSDSEAQDLLLCIQTQKTLDDPNRLRFSSDQFYL
                     KSPEEMIEQLGAIPGAIENTLAIAERCNLNLELGTLHMPSFPLPDGESRSPYEYLRDL
                     VYKGAKIRYRELTEELRDRIEFELDVIHRMKFDSYFLIVWDFVRYAKENGVSVGPGRG
                     SAAGSIVAFCLFISDIEPIQNNLLFERFLNPDRVSPPDIDIDFSDQTRGKVIRYVTEK
                     YGEQNVSQIATFSTIKAKNAIKDVGRVLGVSIAECDRITKIMPNDPNLTLESALTNSE
                     LREIAESDETHRRLFRLASRLEGLIRQTGVHAAGMIISDRNLSEMVPLMRTNKGEVCA
                     QYEKNDLEEIGLIKMDFLGLKTLSFIDECLRLLKEDGIEIDLSAIPEGDELTYDLLGR
                     GVTDGVFQVEGSGMKEMLRKLGPKTFSDIVAMIALYRPGVLGSGMAEDFIKRKNGQDS
                     FEYDHELLEPILRETYGIILYQEQVMQIAHKMGGFTLAEADSMRRAMGKKDKEKLLKM
                     QEKFTTGAMAKGVNKKTAEKVFELIGYFGGYGFNKSHSAAYAVLVYQTAYLKAHYPLH
                     FMAALLTSEKDNSEKLRRYMLSAKEMGIRILPPCVNISGERFTVSDEAIRFGLSGIKN
                     LGEAAVQAILQEREQGGAFTSLMDFCQRVGFQGVNTKVLETLVRSGGFDCFSARRSQL
                     LAVHKDVLDRANSLRQDQAIGQNSLFEMMSDGAPHDEMPLLPDIPELERQEMLRDEKR
                     LLGVHISGHPLDEIAQEIRMLSNVSSETVAEADERRGVKMVGLVTNPVRRLTKKGDPL
                     MVFKLEDLSGSVEVVAYSEVYAASSHLLEEDRIVYLEGDAERRREELTLVASKIISFE
                     EARIRLIRALHVVLELRELDNGVIPRLEMALGKNPGSVPVIFHVRLNEEEEMLIRAGG
                     QIRVKPGTALSGKLDDLISADRYYYEFGFSEKLDYGSPASIGSRRPKE"
BASE COUNT         1808 a         2281 c         1903 g         1850 t
ORIGIN      
        1 tgtttgatac tccagagcag cgcgaacaaa atcccgaaac aacggatggg gatttctcgg
       61 acgggatttg aactccggat ggaattggca ggcaacaaac cagggatgat ccggaatctc
      121 caccatttcg accagattgc cttgaggaca gatgccactg acgatcatac catgctcttg
      181 catctgttca atataggcat tgttgacttc gtacctgtgg cgatgtcgtt ccatgatctc
      241 atcctgtcca taggcacttt gtgctcggct ggacggcttg acggtacacg gccacgctcc
      301 caaccgaagg gtgccgccca tgtccacaat gtcgcgctgt tcagccagca gagacacaac
      361 cggatgagaa gtctttggat caaactcagt tgagttggct ccagcaagcc cgcaggcatt
      421 tcgagtgaat tcaataacgc agcattgcag tccaaggcaa ataccaaaga agggaatcct
      481 gttctctcgg gcatagcgaa ttgcctgaat cttgccttcg atgccacgca ttccgaaacc
      541 gcctggcact aggattccat ctacgtcagc aagaatttct cccgcgggac gctgctcaag
      601 atcctctgca tcgaacttga ctatctccac gcgggagtca ttgccgatgc caccgtggat
      661 gatcgcttca aacagactgc gataggcatc ctgaagctcc atgtactttc cgatcaccgc
      721 gattcgtgca catcgggggg cactcttgat ggtgtccact atcttttgcc agggagacca
      781 gtcgtgttcg tgcgccttca gtccaagttt ctcaacaacg attcggtcca gcccttcctg
      841 acgaagccag gatggaatct catagatcgt ggactgaatg tccacggcac tgatgaccgc
      901 cctcggtgcc acgttgcaga acagtgctat cttctccttc atgtccttgg gaaggggact
      961 acgcgttcgg cacagcagga tatcgggcat aatgccgatc tcgcgcagct ccttgacact
     1021 gtgctgagtc ggtttggtct tcatctcacc agcaacaccg atgtaaggga tgagcgtcag
     1081 gtggatgtag atcacatttt cgcgtccacg ttcctgtccg tactgacgaa tggcctcaag
     1141 aaaaggcact gattcaatgt ccccaacggt gccgcctatc tcacacagtt gcacgtccgc
     1201 atcggtattg cgagcgacag tgagaatgcg tgacttgatc tcatttgtta tgtggggaat
     1261 gacctgaacc gtctgtccca ggtattcgcc gtggcgttca cgctcaataa cggcctggta
     1321 cacgcgtccc gtcgtcacgc tgttgtcccg agacagcggt gccgaagtga accgctcata
     1381 gtagccaaga tccagatcgg tttccgcgcc gtcatcggtg acatagacct caccatgctg
     1441 atagggattc attgtcccag ggtcaacatt cagataaggg tctatcttct ggagcgtcac
     1501 ccgcagtccg ctgctttcca gcagacaacc cagagacgcc gcagccaccc ccttgcccag
     1561 agacgaacag acgccgcccg tgacaaagat agtcttctca atcatttggt tgcctcatat
     1621 aaaggatgcc gcgaaagaag cggagtctgc agactcaacc ggcctctgat ttagacctca
     1681 ttttgcttct gtccatgaca aagaagccgc cagacttcag tctgtgatct gagccggaaa
     1741 cctccttcac ctcatccgta agacacctct tgagcgcccg gctttagtcg ccatcggttt
     1801 ctttggcaag cagagcttcc accttcttaa tatcctccgg tgtatccacg cccaaacaag
     1861 gccagttgcc cttgagaacc ttaatccaga tgccattctc caaagcccgc agctgttcga
     1921 gcttctccgc cttttcaagg cgaccttcag gcaagctgag gaaactcagg agtacttccc
     1981 gccgataggc gtacacgcca acatggtgcc agtactccgg catcttctgg cctggctttc
     2041 ccgcgtcgcg ccagtaagga attgggcttc gcgagaagta aagggcgaaa tcatcgttgt
     2101 cgcagaccac tttgaccgca ttgggattgg ccacatctcg gatctccagc ggtctggcaa
     2161 gtgtggccat ctcaaggttc ggatccagtt tgaactcttc gatcaagcgt tcgatggcac
     2221 gtgggtcaat gagtggctca tccccttgaa cattgatgac gatctgctca tcacgctctg
     2281 caatgacttc tccgacccga tcgcttccag aagcacaatc gggagacgtc atcacggcct
     2341 gtccaccgaa tgcatgcacg acatccgcga tgcgctgatc gtctgtcgcc accaacacag
     2401 cgttgactcg atgtgccatc cgtacccgtt cccaaacgtg ctgaatcagg ggcttcccac
     2461 aaatctcaac caaaggcttt ccagggagac gctgagaacc aaatcgtgct gggatcacac
     2521 aaatagatcg cactgactca ctccttcatc tcatctgatc aagacagcac tcaccagagc
     2581 gcaaaaaaac aatcacacga gcgtccacta cggatcatct ttggtgatgg cttcctgaag
     2641 ctcatggaga ctgaccgttg aaacgcccac cttgccaacc acaacaccgg ccgcgtggtt
     2701 ggacaaatgc gccgcggtca ccagatcaac tcccgaagca aggcacaacg ccaaaacagc
     2761 catcacagta tctccagcac cagtgacatc gtaaacctga cgtgcagtcg tcgggatgtg
     2821 cgtaaagccg tgttcctttt caaacagaga catcccttcc tcgccacggg tgatgagcac
     2881 tgcctgacac tcgacatccc gcaacaaacg ttctgccgca agccgaaggt cttcatggct
     2941 ggagatcaat atatgagcgg cctgtgccgc ttcctgctga ttgggtttga tcaagtccac
     3001 cccacgatag accgagaaat caagcgtctt gggatccaca agaacaggaa tggcacccgc
     3061 gtgccttttg gatgctgcgg aaacaatccc ctcgatcaga tcccgactca cgacaccctt
     3121 cgcatagtcc gcgataacta tcgccttaac acggggcaga caccgttcga tagagtcgag
     3181 cagtttcgtg gtcagactag cggagaccga ttcacgaact tcacggtcta cacgaaccaa
     3241 ctgctgtgaa tgagcgatga tgcgggtctt gagcgtggtt ggcttgcctt tgatggggaa
     3301 aatcccttgg gtgttcagac cgttctccga gagaagatcg agcagaatgc gcccttcgcg
     3361 atcctgtccg accgtgcccg ccacatgaac ctcggcaccc agagcgtgga ggtttgtcat
     3421 cacattggcg gcgccaccag gcacatacgt ttccgactga acgtccacca ccggcacagg
     3481 agcttctgga gaaattcggc tgactttgcc ccagatgaac cgatccagca tgagatcccc
     3541 gacgacaaga acacctgtgc cgtgaagccc tttgaggctt ccaagcattt ttcgctgcat
     3601 catccgcgaa cgatccatgt cacctcctgg aaaagtccag gctaaactta ctgctaatca
     3661 ggccagtgcc ttcaggcgtg tgggctatgc ccgctccttc ggacggttgc ttggcaaatg
     3721 acacgcttct gctgaacggt gtgtcattag gaatgaagcg aggaatccct cctgtcaaca
     3781 agaggctgct gcaatcatag agattcttca ctccgatttg ctccgttcag aatgacatct
     3841 gccactgtac tatgtctcgc gcaaatccct ctgatatcta ccacaagcaa gagggatgtg
     3901 tctcaggatg tcttctcaaa gattcccata cgacgaaact tctcgtaccg ttttctggag
     3961 agatcttctt tcggaatctg atccaactcg gccaaattcc tgagcacggc ttgcttgatg
     4021 gtctctgcca tccggtcgta atcccggtgc gccgcaccca tcggttcggg gagtatctcg
     4081 tcaataatgc ccagccgatg caggtcgccg gagagaatct tcatggattc aacggcttcg
     4141 gctgctttgg ttcggtcttt ccagataatc gcggcacagg cttctggagt gcacactgcg
     4201 taataagcat gttccagcat aatcacccga tcgccgacac cgatggcaag tgcccctccg
     4261 cttccacctt cacctgttac caccacaatg atcggagtgt tgagagcagc catccctaag
     4321 aggttctcgg cgatgatcaa tgcctgccct cgttcttcag ccccgacgcc tggatacgcg
     4381 cctgaggagt caatgaacgt caaaacggga aggccaaaac gctcggccag ctgcatcagc
     4441 cgaagtgcct ttcgaaaacc ttctggatgc gccataccga aattccgctt caggttttcc
     4501 ttggtatcgc gacccttctg atgccccaaa gcaacgatgg gtcttccgtc caggcaagcc
     4561 agtccgccaa cgatcgccgc atcgtcccga tagcaccgat cgccatgcag ttcaaagaag
     4621 tccgttgtca tgcgggtgat gtaatccagg aagtaaggcc tgtctggatg acgtgccaat
     4681 tggatcctct gccaaggtgt caactggctg aagatgctct tctcaacttc ctgcagcttt
     4741 tccttcatgg cgctgatctc ggtggataga tcaatgcccc gttccgaagc ataccgcgtc
     4801 atttcctcta tcttgctctg aagctccacc aagggtcttt caaaatccag tgctcttcgc
     4861 ataatcgttc atcctatcgg cgacagggcc gtcggactgt cctttcgtgt atttgcatgc
     4921 cgacaagcat gcctctttct cgtcagccgg gctcactcct taggtcttct acttccaata
     4981 gatgccggac ttccatagtc cagtttctcg gagaagccaa actcatagta ataacggtcc
     5041 gcgctgatca agtcatccag tttgccgctg agtgctgttc cgggcttaac ccgaatctgc
     5101 ccccctgctc gaatcagcat ctcttcctct tcgttgagcc gaacgtgaaa gatgaccggc
     5161 acggaaccgg ggtttttccc gagagccatc tccagtcttg gaatgactcc attgtccaac
     5221 tcccgcaact ctagcacaac gtgcagtgct cggatcaatc ggattcgtgc ttcctcgaaa
     5281 ctaatgatct tggaagctac taaggtaagt tcttcccgac gccgttccgc atccccttcc
     5341 aagtatacaa ttcggtcttc ctcaagcaaa tgcgacgagg ctgcatagac ctctgaatag
     5401 gcgaccacct cgaccgatcc ggacagatcc tccagtttga aaaccatgag aggatcgcct
     5461 ttcttggtaa gtctacgaac cggattggtc accagcccca ccatcttgac tccccttcgt
     5521 tcatctgctt ccgcgacggt ttcgctcgaa acattgctga gcatccgaat ctcttgagcg
     5581 atctcatcca gaggatgacc ggagatgtga actcccagga gtctcttttc atcgcgcagc
     5641 atctcttgcc tttccaattc ggggatatcc ggaagaagag gcatttcatc atgtggtgca
     5701 ccatcggaca tcatctcaaa gagcgaattc tgtccgattg cctgatcctg tcggagcgag
     5761 ttggcacgat ccagaacatc cttatgtact gccaacagtt gcgaccgtct ggcactaaag
     5821 cagtcaaatc cccccgaacg aaccaatgtc tcaagcacct ttgtgttgac gccctggaat
     5881 ccaactcgtt gacagaaatc catcaacgaa gtgaaagcac caccctgttc gcgctcctgg
     5941 agaatcgcct gaaccgccgc ttcgcccaga ttcttgatgc cgctcagacc gaatcgaatc
     6001 gcctcgtcag aaacagtgaa gcgctctcct gaaatgttca cgcatggggg aagaatgcgt
     6061 attcccatct ctttggccga gagcatgtaa cggcgcagtt tctcagagtt atccttttcc
     6121 gaagtgagaa gcgccgccat gaagtgaagc ggataatggg ctttgagata cgccgtctga
     6181 tagaccagta ctgcgtatgc cgcgctgtgg gacttgttga aaccataacc gccgaaatag
     6241 ccgatcagct cgaagacctt ttcggctgtc ttcttgttga cacctttggc catggcaccc
     6301 gtggtaaact tctcctgcat cttgagaagc ttttccttgt ccttttttcc catggcgcgc
     6361 cgcatcgagt ccgcttctgc cagagtgaaa ccacccatct tgtgggcgat ctgcatgacc
     6421 tgttcctggt agagaatgat accgtacgtt tcccgaagaa tgggttcaag cagttcgtga
     6481 tcgtattcga acgagtcctg tccattcttg cgcttgatga aatcttcggc catgccactg
     6541 cccagaacac caggacgata caaggcgatc atggccacaa tgtcactgaa cgtcttgggt
     6601 cccagttttc ggagcatttc cttcatgccc gaaccttcca cctgaaacac gccgtctgtc
     6661 actccgcggc caagcagatc ataagtgagt tcatcccctt caggaatagc cgaaaggtct
     6721 atctcgatgc cgtcttcctt cagcagacga aggcattcat caatgaaaga cagtgttttc
     6781 aaccccagga aatccatctt gatcagcccg atttcctcaa ggtcattctt ctcgtactgg
     6841 gcgcagacct cacccttgtt ggtgcgcatc aggggaacca tctccgacag atttcgatcg
     6901 gagatgatca ttcccgcggc atgcacaccg gtttgccgaa tcaacccttc cagtcgggag
     6961 gcaagtcgaa aaagtctcct gtgggtttca tcggactccg caatctctcg gagctcggaa
     7021 ttcgtgaggg cggattccag tgtcagattt ggatcattgg gcatgatctt ggtaatgcgg
     7081 tcgcattccg caatgctgac cccaagcaca cgtccgacat ccttgatggc gtttttggcc
     7141 tttatcgtgc taaaggtggc aatctgagag acgttctgct ctccgtattt ttccgtgaca
     7201 tagcggatga ccttgccgcg tgtttgatcg ctgaagtcaa tgtcaatgtc aggagggctg
     7261 acacggtcgg gattcagaaa tcgctcaaac agaagattat tctgaatcgg ttcgatatct
     7321 gagatgaaaa gacaaaaagc gacaatgctc cccgcggcag aacctcgccc cggccccacc
     7381 gagacgccat tttccttggc atatcggacg aaatcccaga cgatgagaaa atacgaatcg
     7441 aacttcatcc gatggataac atccaactcg aattcgatgc gatcgcgaag ttcctcggtc
     7501 agttcccgat accgaatttt ggcgcccttg tacacgaggt cacgaaggta ttcataggga
     7561 cttctgctct cgccatccgg cagagggaac gagggcatgt gaagtgttcc caattccagg
     7621 ttcaaattgc agcgctcggc gatggccaga gtgttttcga tggctccagg aatggcgccc
     7681 agctgctcaa tcatctcttc tggagacttg aggtagaact gatcggagga gaaccgaagc
     7741 cgattcggat catcgagtgt cttctgcgtc tgaatgcaca ggagcaggtc ttgagcttcg
     7801 ctgtcactcc gcagcagata gtgacaatcg ttggtggcta cc
//
LOCUS       DAOQUC010000011         7746 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_10389_length_7746_cov_3.282668, whole genome shotgun sequence.
ACCESSION   DAOQUC010000011 DAOQUC010000000
VERSION     DAOQUC010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7746
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10389_length_7746_cov_3.282668"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..965)
                     /locus_tag="PKH07_00410"
     CDS             complement(<1..965)
                     /locus_tag="PKH07_00410"
                     /inference="COORDINATES: protein motif:HMM:NF012551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HNT33444.1"
                     /translation="MQRTLNAMYRGALFLILVTAALTGLPLYGSCSTEEIQIVFDSPN
                     GIYSAGVVTQVKIQVVRASSVAAQKHVTLTVTNSLGKTINSQKGWITAQADGTFSIVF
                     DVPVTETGYYEVKATVTPASDVQTAKSGFVILAPRSESGVPSENSIFGLSGGAIFHLP
                     AESAAQVGTERLSKFPLLGCTWGRNDLWWGTIEPKQGQWDWSRADLTVKLFKDNGVQL
                     LGILCYSSAWSGGKAPATADEVERFAAYVTATVSRYKDSIKHWEVWNEPNLAEFWSPE
                     PNVSDYATLLKAAYSAAKSADPSAFILGGVTAGVDLRFLDSLFSMG"
     gene            1088..2380
                     /locus_tag="PKH07_00415"
     CDS             1088..2380
                     /locus_tag="PKH07_00415"
                     /inference="COORDINATES: protein motif:HMM:NF016612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HNT33445.1"
                     /translation="MKKEFPVRAGTARTEITPPIGVDLSGYAARENPSKGINDPLFTK
                     ALALEAGGEVIVLVASDLVGLSPQSVHRIRQDVFSRTTVLSERLLLTCTHTHSGPATV
                     ALRECGTPDDTYVRKLEGAIASNIVQALDSLQSARIGFGHGQVMAPVFLNRRQPDGGV
                     VDPEVVVLRVDTEDGSPIATWINFTCHAVVWSGENLNISADFPGAVAALVEQKIGGLA
                     LFSNGACGDINPNARGSDAIASVATPIADEVLRVWGAIKTSPTASLAVDRELFSIPFA
                     PLMDRCEAEEQLRTWQKALEEASGVGVHGRIARAHIQMAEAVLALHRKGQTPAALEAD
                     LKVTSIGDHFLVSFPGEVLVQIGLCLKEEAPGRVAFLGYTDGCLGYIPLSEDFQKGGY
                     EPAAAWVYYGHNAPFEVGVGERLVKSAREMLKQRLAER"
     gene            2426..3664
                     /locus_tag="PKH07_00420"
     CDS             2426..3664
                     /locus_tag="PKH07_00420"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNT33446.1"
                     /translation="MGKLAVNGGTPVRTKPFPSWPVFGDKEEKALVEVLRSGSWGLPG
                     IKAREFEQKFSAFQHAEFGVACCNGTIAIEIALLALGVGAGHEVIVPPYTFMATATAV
                     VTVNAIPVFVDIDPDTCNISPEAIEKAITPHTKAIIVVHVAGCPCDMDAILAIANKHG
                     LKVIEDSAHAHGAEWKGKRVGALGDMGTFSFQSSKNLTSGEGGIIVTNNPELADLAES
                     IHNCGRAKGKGWYEHFQIGANYRLSEFQAAILLCGLERLPEKMAIRERNGALLDKGLA
                     QIPGVAPQKRDPRVTSHGHHLYMARFPQQDREGIPLARIIQALNAEGFPCSTGYNPLY
                     KLALYSEAHFGPHGCPISCPFHKGTKPDYSKIMLPEAEKAADEIVWSHHPVLLGSAED
                     TQDIVDAVAKVMENKGELIA"
     gene            3690..4880
                     /locus_tag="PKH07_00425"
     CDS             3690..4880
                     /locus_tag="PKH07_00425"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNT33447.1"
                     /translation="MKKRGLLLGCFVLVAVLVVSGCAKQTPEELFVKGCKAFEGGDVI
                     GAALYFNKFLENHAEHEYAGRAHLMLARTRARLGDEALAIKEYRVVTRKFPDSPEAVN
                     ASFELAEYYAMTQRPDEVEKELRALYQRSQDSRIQARALFEIAGAYISNATNPQKAES
                     ILREALLIAEDDEIKINAFFGMAEAYIRCATEPQKTEAILREALPLTTKTEERGEALF
                     RMGHAYVACHDYEDAIRIYNEIIHDASVEQESKAMGYLFLAKSHKAEGDQVQALETLE
                     QLRDTLGTTDVAIWSNVESALIVRETTPDQFDGYLSRAISAYQEIMDVSPTMDRAAWA
                     LTKIAEAHREVGDITSSIQTYGQIIEQHQDKEPYVDRSRQMIRILRQQLEASPETVPS
                     ATEG"
     gene            4896..6347
                     /locus_tag="PKH07_00430"
     CDS             4896..6347
                     /locus_tag="PKH07_00430"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNT33448.1"
                     /translation="MELLWKLKKVLLCHPPSGLYRRDDRCQSKVDDQTVRVVLPPLDL
                     AYHAAVLERLGCQCKIADYPSEHLTWENFEKDVREFRPDLLLISATYPTLASDLEAAK
                     RLKTLLPECLTVARGEVFSVWDKLTLQKYPHVDMVLTGESELSLAALVDGRSPVDIPG
                     LTYRYGEQILRGPEPELIADLDALPFPARHLLKNHLYRSPDSNQLLTTICAARGCPSE
                     CIFCPVRRVSGVKIRLRSPENILEEIQECVEKYHIRDFLFHADTFTYHKRWLIELCRL
                     IADQDLEIRWGCNSRVDTIDEERIFWMKRAGCWIIGFGVESGDEQSLLWMRKNASLDQ
                     ARKAISLCRKYGVRSHAFFMFGFPWDTHQTIRQTVAFAKELSPDFFDFNIAFPLPGTE
                     FYEMAKEEGLLVFPEGEEAEGYAKAIVRTRTLSPEDLERWRTHALWRLYLRPGYILKT
                     LWKTRSLPVALNYVKAAMRRGRGLLKKKSNPQP"
     gene            6503..7105
                     /locus_tag="PKH07_00435"
     CDS             6503..7105
                     /locus_tag="PKH07_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNT33449.1"
                     /translation="MSTDQGNDFRPSDFLESDVFADVNSCGEKRCMDAKRLIELLGLT
                     PHLAEGGYFKETYRSIERIPEDGLPARFRSARNLATAIFYLLTPDTCSAFHRLQSDEV
                     FHFYLGDPVTLVMLYPNLLSERIVLGSDLESGQSVQCLVPRGTWQGMFLNDGGKFALM
                     GTTVAPGFEFDDFECGDRDILLSEYPQEASAIVRLTRSSR"
     gene            7233..>7746
                     /locus_tag="PKH07_00440"
     CDS             7233..>7746
                     /locus_tag="PKH07_00440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33450.1"
                     /translation="MRDFVKLFLTVAILSAIIAVASSSVFAGDVYEWDSSSVRLSPAH
                     DLPALLSDSELAGARPLDGALRNLLAGQLLAVRAGETLAGGEQGTFEFHAKTYLTLDD
                     KPLVDITVVGDAKATERELKSIPGVIMGSSASTPAYTLISAAVPVSDLLAVASAPNVR
                     QVVASRVIRPE"
BASE COUNT         1785 a         2035 c         2120 g         1806 t
ORIGIN      
        1 cccatcgaaa aaaggctgtc gagaaaccga agatctacac ctgccgtgac accaccgaga
       61 atgaaggccg agggatcggc tgacttagct gccgaatacg ccgccttgag gagcgtggcg
      121 taatccgaaa cattcggttc agggctccaa aactctgcca gattcggctc gttccagact
      181 tcccaatgct tgatcgagtc tttgtatcgt gaaaccgttg ccgtgacata cgctgcaaat
      241 cgttccactt cgtctgctgt cgctggtgcc ttgccaccag accatgcgga gctgtagcag
      301 agaatcccaa gcagttggac cccgttgtcc ttgaagagtt tgacggtcag atccgcacgg
      361 ctccaatccc attgtccctg ttttggttca atggttcccc accagagatc attgcgtccc
      421 caggtgcatc cgagcagcgg aaacttggac aaccgttccg tacctacttg tgcagcgctt
      481 tccgccggaa ggtgaaagat cgctccaccg gagagtccaa agatggagtt ttccgatggg
      541 actcccgact cagaccgagg agccagaatg acgaagccac tctttgcagt ctgaacatca
      601 ctggctggag tcacagtcgc cttcacttcg tagtagcccg tttccgtaac aggaacatca
      661 aagactatcg aaaatgtccc atcagcctgc gctgtgatcc aacctttttg tgaattgatg
      721 gtcttgccca gagaattggt gacggtaagg gtcacgtgct tctgcgccgc cacagaactc
      781 gcccgaacga cttgaatttt aacttgagtc accactccag ctgagtaaat gccattgggt
      841 gagtcaaaga cgatttggat ctcttcagtg gaacacgaac cgtaaagcgg cagacctgtc
      901 agggcagccg taaccaaaat cagaaacagt gctcctcgat acattgcatt caacgttctc
      961 tgcactcctt ctccttaaat cctcagatag agcattctat ctcaccgttc tgttcttgat
     1021 caaacccgtc attcggacta tattcattgt tcatgaaata gacgaccttg aaaccaagga
     1081 taacagtatg aagaaagaat ttccagtcag agctggaact gccagaacag agatcactcc
     1141 accgataggt gtggatctct ccggttacgc cgcgcgggag aatccttcca aagggatcaa
     1201 tgatcctctt ttcaccaaag cccttgcctt ggaagcgggt ggagaggtga tcgtattggt
     1261 tgcgtccgac cttgtcgggc tgagtccgca gagcgtccac cgaatccggc aggatgtctt
     1321 ttcacgaacc actgttcttt ctgaaaggct cctgctcaca tgtacacaca cccactcggg
     1381 acccgccacc gttgccttga gggagtgtgg aacccctgat gacacttacg tgaggaaact
     1441 cgaaggtgcc atcgcctcaa acatcgtgca ggcgctcgat agtcttcaat ctgcccgaat
     1501 tggatttggc catggccaag tcatggctcc tgtgtttctg aaccgccgtc agccggacgg
     1561 aggcgttgtg gatcctgaag tcgttgtgct tcgcgtggat acggaggacg gaagcccgat
     1621 tgccacttgg atcaacttca cctgccatgc agtcgtatgg tcgggagaga atctcaatat
     1681 ctccgcggac tttcctggtg ccgttgcggc gcttgtggag cagaagattg ggggacttgc
     1741 acttttctcc aatggagcct gtggtgatat caaccccaac gcgcgtggca gcgatgcgat
     1801 cgcttcggtc gcgactccca ttgccgatga agtgcttcgg gtctggggtg ccatcaagac
     1861 cagtcccaca gcttctctcg cggtggatcg ggagcttttc tctattccgt ttgcaccttt
     1921 gatggatcgt tgtgaagcag aagagcagct gcggacgtgg cagaaagcct tggaagaggc
     1981 gtccggtgtg ggtgtccacg gtcgcattgc acgcgcccat atccaaatgg cggaggcagt
     2041 gctggcgttg caccgaaaag ggcaaacccc tgctgcacta gaggcagact taaaggtcac
     2101 ttccattggc gatcactttc tcgtcagttt tccaggggag gttttggtac agatcggtct
     2161 gtgtctcaag gaagaggcac ctggtcgagt cgcttttctg ggttacacag acggatgtct
     2221 cggatacata ccgctgagtg aggatttcca aaagggtggc tacgagccag ccgccgcttg
     2281 ggtttactat ggccacaacg ccccttttga ggtgggcgtt ggcgagaggc tcgtcaaatc
     2341 ggcgagggag atgctgaaac aacgactcgc ggagagatga tctgtccttc cgccagcgga
     2401 ggcacacccg caaggagaat gtagaatggg taaattggct gtcaacggag ggactcctgt
     2461 cagaacgaaa ccgttcccca gctggcctgt ctttggagac aaagaggaga aggcgctggt
     2521 ggaagtgctg cgaagtgggt cctggggact tcctggcatc aaggcacggg agtttgagca
     2581 gaagttttca gcgtttcagc atgcagagtt cggagttgcc tgctgcaacg gaaccatcgc
     2641 catcgagatt gccctcctgg cactgggggt gggtgccggc catgaagtta ttgtgccccc
     2701 ctatacgttt atggcaacag caacggcggt cgtcacggtc aatgccattc cagtgtttgt
     2761 ggacattgac ccggacacct gcaacatcag ccctgaggcc atcgagaagg cgatcacgcc
     2821 tcacaccaaa gcgatcatcg tggtgcatgt ggccggttgt ccctgtgata tggatgccat
     2881 tctggctatt gccaacaagc acggtctgaa ggtaatcgaa gacagtgcac acgcacacgg
     2941 tgcagaatgg aagggcaagc gtgtcggtgc ccttggcgac atgggaacgt tcagtttcca
     3001 gtcctccaag aacctgacgt ccggcgaggg tggaattatc gtgaccaaca atcctgaact
     3061 ggcggatctt gcggaatcca ttcataactg tggtcgcgcc aaaggtaagg gttggtacga
     3121 gcattttcag attggtgcca actatcgcct ctcggaattt caagccgcga tcctgttgtg
     3181 tggcctggaa cgccttccgg aaaaaatggc cattcgggag cgcaacggag cccttctgga
     3241 caaagggctt gcccagattc ctggtgttgc gccacagaag agggatccgc gtgtgaccag
     3301 ccatggacat catctttaca tggctcgatt cccgcagcaa gatcgcgaag gcattcctct
     3361 tgcacggatc atccaggcac taaatgcaga aggttttccg tgcagtacag gttacaatcc
     3421 gctctacaaa ctggcgctct acagtgaggc gcacttcggc cctcacggat gtccaatttc
     3481 ctgtcccttc cacaaaggaa ccaagccgga ctactccaaa atcatgctgc cagaggcgga
     3541 gaaagcggct gacgagattg tttggtcaca tcatcctgtg ctgcttggtt cggcagagga
     3601 cacacaggat attgtagacg cggtggcaaa agtcatggag aacaagggcg agttgattgc
     3661 gtgattggag cgcggaggtc gcaagtacga tgaaaaagag gggactcctg ttgggctgtt
     3721 ttgttttggt tgcagtcctg gtcgtttcgg gatgtgccaa gcagacacca gaagagctgt
     3781 tcgtcaaggg ctgcaaagcc tttgaggggg gtgatgttat cggcgcggcg ctctacttta
     3841 acaagtttct ggagaaccac gccgaacatg agtatgccgg aagagcacat ctcatgctgg
     3901 cgcgtacgcg ggcgcgactg ggggatgaag cacttgcgat caaagagtat cgggtcgtca
     3961 cgcgaaaatt cccagactct cccgaggcgg tgaatgcaag ttttgaactt gcggaatact
     4021 atgccatgac ccagcggcct gacgaggttg agaaggaact tcgtgccctc taccaacgaa
     4081 gccaggacag tcggattcag gcgcgcgcac tttttgagat tgctggtgcc tacatcagca
     4141 acgccaccaa cccccagaaa gcggagagca tcctgcgaga ggccctgttg atcgcggaag
     4201 acgatgagat caagatcaat gcttttttcg gcatggcaga agcgtacatt cgttgtgcaa
     4261 cggaaccgca gaaaactgaa gcgattcttc gggaagcctt gcctctgaca acaaagaccg
     4321 aggaacgagg cgaagcgctg tttcgaatgg gacatgctta cgttgcctgc catgactatg
     4381 aagacgcaat ccggatctac aatgagatca tccatgatgc gagcgttgaa caggaaagca
     4441 aagccatggg atatctcttt ctcgccaaga gtcataaggc cgagggcgat caggttcagg
     4501 ctctggaaac cctcgagcaa cttcgcgaca cgttgggtac aacggatgta gcgatctggt
     4561 caaacgtcga gagcgctttg atagttcgtg aaaccactcc tgaccaattt gacggttatc
     4621 tttcacgcgc cataagtgcc tatcaggaga tcatggatgt ttcgcccacc atggatcgcg
     4681 ccgcgtgggc attgaccaag attgcggagg cacatcggga agttggtgac attacctcgt
     4741 ccattcaaac gtacggacag atcatagagc agcatcagga taaggaaccg tacgtagacc
     4801 gctcgcgcca gatgattcgg attctgcgcc agcagttgga ggcatcccct gaaaccgtcc
     4861 catcggcaac cgagggctga gagcgtttta tctgcatgga actactttgg aagctgaaga
     4921 aagtcctttt gtgtcatccg ccttcgggac tttacagacg agatgaccga tgtcagtcga
     4981 aagtggacga tcagactgtt cgggttgtgc ttccccctct cgacctggcc tatcacgccg
     5041 ctgttcttga gcgtctcgga tgtcaatgca agattgctga ctatccttct gagcacctca
     5101 cctgggagaa cttcgagaaa gacgttcgag agtttcgtcc tgatttgttg ctgatctcgg
     5161 cgacgtatcc aactctggcc agcgatctcg aggccgccaa gcgattgaag accctcttac
     5221 ccgaatgtct taccgttgca cggggggaag tgttctcggt ctgggataaa ctgacactgc
     5281 agaagtaccc ccatgtggat atggttctga cgggagaatc tgaattgtcg ctggccgcct
     5341 tggtggacgg tcgttctcct gttgacattc ctggattgac ataccgctac ggagagcaga
     5401 ttctccgagg tcctgaaccg gaactcatcg cggatttgga cgcgctgcct ttccctgccc
     5461 gacatctgct caagaaccat ctctatcgaa gtcccgacag caaccaactg ctgacgacaa
     5521 tctgtgccgc gcgaggctgt ccttcagagt gcatcttttg cccagtcaga cgggtctccg
     5581 gcgtgaagat tcgcctgagg tcgcccgaga acattcttga ggagatccag gagtgtgttg
     5641 agaaatacca cattcgggat ttccttttcc atgcggacac gtttacgtac cacaagcggt
     5701 ggctgatcga actgtgccgg ctcatcgctg atcaagactt ggagattcgt tggggatgca
     5761 acagtcgggt ggatactatt gatgaagaac gcatcttttg gatgaagcgt gcggggtgtt
     5821 ggatcatcgg ttttggcgtt gagagtgggg atgagcagtc cctgctctgg atgcgtaaga
     5881 acgcctcgtt ggatcaagcc cgcaaagcga taagcctctg ccgcaagtat ggtgttcgtt
     5941 cccacgcttt cttcatgttt ggttttccct gggataccca tcaaaccatc cgacagaccg
     6001 tggcttttgc gaaggaacta tcaccagact tctttgattt caacattgcg tttccgcttc
     6061 cgggaactga attctatgaa atggcgaaag aggaaggtct tctcgtgttt cccgaaggag
     6121 aagaagccga gggatatgcc aaggccatcg ttcgcacccg aaccctgtcg ccggaagatc
     6181 tcgaacgatg gagaactcac gcactgtggc gcttgtatct cagacctggg tacatactca
     6241 aaacgctctg gaagactcga tcgctgcctg ttgccctcaa ctacgtgaag gcggccatgc
     6301 gacgcggacg aggccttctc aaaaagaagt cgaacccaca accgtaacga gacacagaga
     6361 tacacgttcc gtgttcaacc tttggcttgt cacgccgccc agcaggttag caccttcggg
     6421 cgcgtgggct atgcccactc cctcatacgc ttgcttaata aatgacacat gcctttcgga
     6481 ctctgtgtca ctaggagcat tgatgagcac agaccagggc aatgactttc gaccgagtga
     6541 tttcctcgaa tctgacgtgt ttgcagacgt caacagctgc ggagagaagc gctgcatgga
     6601 cgcgaaacga ctgatcgaac tacttggcct cacacctcat ctggcagaag gcggatactt
     6661 caaagagact taccgcagca tcgaacgaat tcccgaagac ggtcttcctg cacgattccg
     6721 ctccgcgaga aatctcgcga cagcgatctt ctatcttttg actccggata cctgttctgc
     6781 gtttcatcgt ttgcagtctg atgaagtctt tcatttctat ctcggagatc ccgtcacgct
     6841 ggtcatgctc tatccgaacc tactcagtga acggattgtt ttgggttccg atctggagtc
     6901 gggtcagagt gtacagtgtc tggtgcctag aggaacttgg caaggtatgt ttctcaacga
     6961 cgggggaaaa ttcgcactca tgggcacgac agtggcacca ggtttcgagt tcgatgattt
     7021 cgagtgtgga gaccgcgaca tactcctgtc agaatatcct caggaagcct cggcaatagt
     7081 ccgtctcacc cgatcttcca gatagatgag cggtcacgct tcctaactga tctaaaaaat
     7141 gcggttcttc ttcagaaaaa gctacacaga gtcatgccca ttaggctaga ctccaaaaat
     7201 gtgtctgatt ttttgacaag gcggtatacg agatgcgaga ctttgtaaaa ctgtttctca
     7261 ctgtggccat actttcagct atcattgctg ttgcctcatc ttccgttttt gccggagatg
     7321 tttatgaatg ggactccagt tctgtaaggc tttcacctgc tcacgatctt cccgcgcttc
     7381 tttcagactc agaacttgcc ggcgcacgcc ccttggatgg tgcgctgcgg aatcttcttg
     7441 ctggacaact gttggccgtg cgcgcaggag agactcttgc ggggggagag caagggactt
     7501 ttgagtttca cgccaagacc tatctcacgc tggacgacaa acccctggtg gacatcacag
     7561 tcgtaggcga tgccaaagcc acggagcgcg aactgaaatc aatccctggt gttatcatgg
     7621 gatccagcgc ctcaacacca gcctacaccc tcatttcggc agccgtacct gtttcagatt
     7681 tgctggcggt ggccagtgca ccgaatgttc gtcaggtggt agcctcaagg gtgatccgtc
     7741 ctgaag
//
LOCUS       DAOQUC010000012         7544 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_10764_length_7544_cov_3.045934, whole genome shotgun sequence.
ACCESSION   DAOQUC010000012 DAOQUC010000000
VERSION     DAOQUC010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7544
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10764_length_7544_cov_3.045934"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>7544)
                     /locus_tag="PKH07_00445"
     CDS             complement(<1..>7544)
                     /locus_tag="PKH07_00445"
                     /inference="COORDINATES: protein motif:HMM:NF019325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNT33451.1"
                     /translation="LHRINCLADPDDEIIEINEMNNRAVKNIFVLIPNVPDLVISAAD
                     IHFDPEHPLPGNTVKITASVRNVGTIPANGAVVQIFDGNPLNPLSRQLGSNLITNVAA
                     GSVVEMVYDWVTFATDEGSHSIFVRLDPDDDIDEYSEVNNIASTFLYIGNRRPTLAWS
                     GDYQGYWYLPYASYPYHHNGVEPMIGYQDTLFEYRVTYADPDNNPPADGFPKVYIDIN
                     GDGDENDEGVDFREGGYQMTKVNPAATNFQSGVVYSYKTKLPMGNRARYRFAATDING
                     GEARYEPRTLWQNEGPDVVKSTIRVNVMPEYTTWAGRGNVQVWGNVHWAVGSEGGSYI
                     WDFGDGSQPAFGAVVDPLYIAVEHDYKSIGTYYATLTVVDRKGSSDSDTVKINVVADG
                     TESRRKGMIQDALRFLYLEQEKSSGMWRAAGHDDSATALVLLAFIDNDFLPMDDYDQS
                     IYAEYLAAGFRALCGMGVYSDLSGYPLFDGDRDGVGYRYTSVDPYKTGIALMALTSDR
                     ETTIYNGYIQDLADWIVAAQTSIGGWQYGFRAGTDNSGSQWAALGLWGAMQCPFNAVV
                     PEWAWDSHHPDDPYGVGFLDWLEYSQNVTTNPSTNGQFGYSGRGGGSPSMTAAGFIEY
                     FLCGIPFEGIPDEYFFDVQSMPSGQYYIYLKIDDGVNPPQYVYSPGPFTVLENKPKIT
                     IVDPATGNWPADKSYYITWTDSDRDSNASISLYADDDNAGFDGDEILIASGISEDDLL
                     DYTLWDSSSVPGGSYYILGRITDDGGATYYDNYSTGRVVVSHNSPPTIVVLEPGSDGV
                     DLKEDASLYTIRWVGYDPDSTNVKVSLYWDNNNSGVNGTLIADGLTLQDGIVSSYNWD
                     ISQLSAGTYYFYAVAEDGDNVYVADYAVETMRLNRPPSFRITEPDGVGDTAENTYTIR
                     WEGSDPDPTQVTLFYDTNDSGLDGTEIAKANLGSRSYAWNTRDMLPGDYWIYGLAEDG
                     VNSAVISYSSGPLTITAHPPYISMKSLASADETVDFGFVIEWEDMDVDSSAWIDLVAD
                     NDTNTANGWPISIVSGLSEDSLQDYYAWNTSDVTPGQYYLVARITQDGTTYYESRSSR
                     RLTIVRDGRPSLTMVAPKTGQQRLWGESPSVNLQWYAWDTIASATVSMYYSTDQSYGS
                     DVLIQDASNLLIQHGQLATYAWDMTSVPSGTYYVYGSLQSATQQVRDFALGSLEVLKR
                     PSIQLLQPATVKQATVEAQFQYITWDDTEDSRRPKISLYCDTDNVGQNGTLITDTLTG
                     QSSDSYTWDVTRVPAGEYYIYAIIDDGVNIPVVSYSAGTVVVTSHIPRFEFLEPAPSN
                     VVVDKGGLVQWNAFDIDSPEATIDLYYNTSRTVSGSIPFVTGTLRVNQAPYHFLCDTS
                     LLPEDLYYILARLSDDGRTTYRDYWSSYRFDVQHRGEPAAVAITPLASDVVTSTSLEI
                     QWNAIDLDDNAVVSLYRTSESGTSPSLIVGGLSENDGVAGSYVWSVTDVSSGRYRIRV
                     SVDDGKNAVFSDLSNGRVYINRPPTFNFTKPVVEDDPADATYRLTWTDGDGDTNLSDG
                     AKIALYYDTDTVGYDGTLIVSNIAVDDNANAYTWDTSEVPAGRYYVYAVVDDSVNPAI
                     SVYSTGSVKVADNHAPTMTVALPKTQGEKVDDIYVLLWTDKDWDSDAQIDFYLDTDKT
                     GYHGNEIPIVSGLSEDNEDDYYIYKDIANVTAGLYYVLGRITDGSPVFNSYSVNAMAL
                     SHDISSATNNKKPFLQFVSPRVEGENILGGSREFTIRWVAFDAENAATTTVNIYYDSD
                     GSGANGTLIASALPITNNGGLHTFEWNLSQVPPQTMYLYAEIFDSVNDPVVVYSLGTI
                     TYNLPPTISVVKPDGVSDEVVEQLRILWDDEDLGDNAQISIYWDDDNQGFDGQLIASN
                     INENDGNGPKMAFDWIQYRYQDYMRPEIAQNQGEYLPNFYFTYGFAKGMRLYERNEID
                     QRDWDADLVKFLWDTPYRYDDGSWTSNAYGGSSSDREIFTSWGILSMTKAVLGTKPIA
                     NAGEDKIVGPEDTVAFDGSASFHPNKRRKIIKWEWDFNNDGIFDAEGVTISRSVAQLF
                     NKKIGTYVVTLKVSDDDPCSPVSNTDQLLVRVTNGNRPPIAAITAPNQGFIGFEVTFD
                     GSSSSDPDLGQGISKYEWDLDGDGYFDDGTGPVATRVFSRKQIATVGLRVTDTDIQDP
                     LSNETRITIDIVDAELDLLAQGDAPDPFSPGASPGVRDTNVISFDVSKTSNIRADIFD
                     PAGTLVRRLGPTISQSGGNTFSWDGKATDNTLVPNNLYTYKLGTDAAVLYPELGYRRE
                     AGIRVDGSPADNFVSDPTVARLKDLSLRMHYSAGDGTNNVLSSAISTDGLSWTPEGSV
                     IAPGGTADTVATGSGSPVSLVPYIENNWAAGVGVAFATGERVDTSGGVISLAGGEQNL
                     ALAASYVYAATTQPENPFPDSSGKELNDGRLPDARYHVESGWMGWYNRTGIVVFDLKQ
                     IRDVGLVRLYCKTDHATDVYAGRVVRYPDYITMQA"
BASE COUNT         1839 a         2105 c         1868 g         1732 t
ORIGIN      
        1 gcctgcatgg tgatatagtc cggatatcga acgacacgac ccgcgtagac gtctgtcgca
       61 tggtcagtct tgcagtaaag acgaaccaga cccacatcgc gaatctgttt gaggtcaaag
      121 acaacgatcc cagtcctgtt gtaccaaccc atccatccgc tctcgacgtg atagcgtgca
      181 tccggcaaac gaccgtcatt gagttccttg ccgctcgagt ctgggaacgg attctccggc
      241 tgcgtcgttg ccgcatacac atacgatgcg gcaagcgcca gattctgctc accacccgcc
      301 agcgaaatca caccgcccga cgtatcaaca cgttcgccgg tggcaaaggc cactcccaca
      361 ccggcagccc agttgttctc gatataggga acgagactca caggtgaacc actgcccgtc
      421 gcaaccgtgt ccgcagtgcc gccgggagca atgaccgaac cctctggcgt ccaactcagc
      481 ccatcggttg agatcgctga actgagcaca ttgttggttc cgtctccagc agaatagtgc
      541 atccgcaaag acagatcctt cagcctggcg acggtcggat cagagacaaa gttgtctgct
      601 ggtgaaccgt caacacgaat tcccgcttcc cttctgtacc cgagttccgg gtagagaacc
      661 gccgcatcgg tgccaagttt ataggtatag agattgtttg gcaccaaagt gttgtcggtg
      721 gctttgccat cccaactgaa ggtattgcca ccagactgac tgatcgtcgg ccccagtctt
      781 cggacaagcg ttccagccgg atcaaagatg tcggcgcgaa tgttagacgt cttcgaaacg
      841 tcgaacgaga tcacattcgt gtctcgaaca cccggcgaag cgccaggcga gaacggatca
      901 ggagcgtcac cctgcgccag aagatccaac tccgcatcca caatatcaat cgtgattcga
      961 gtctcgttac tcaagggatc ctgaatgtcc gtatccgtaa cacgaagccc gactgtcgca
     1021 atctgtttgc gactgaacac cctcgtggcc accggacccg tgccatcgtc aaagtatcca
     1081 tcgccatcca gatcccattc atatttcgag atgccctgcc caagatccgg atcgctcgag
     1141 ctgctgccat cgaaggtgac ttcaaatccg ataaagccct gattcggcgc ggtaattgcg
     1201 gcgatcggag gtctgttgcc atttgtcacc cgaacaagca attgatcggt attggagacc
     1261 gggctgcacg gatcatcatc acttactttg agagtcacca cataggtgcc tatcttcttg
     1321 ttgaacagct gcgcaacact ccgactgatc gtcacacctt ccgcgtcaaa aatcccgtcg
     1381 ttgttgaaat cccattccca cttgataatc ttccgacgct tgttggggtg gaaggaagcc
     1441 gatccgtcaa acgcaacggt atcctcggga cccacaatct tgtcctcgcc agcattggcg
     1501 ataggcttgg tgcccaagac ggctttggtc atggaaagaa tgccccagga ggtaaatatc
     1561 tcacggtcgg aactacttcc accataggcg tttgaagtcc aactgccatc gtcgtagcga
     1621 tacggggtat cccagaggaa cttcaccaaa tcagcatccc agtcgcgctg atctatttca
     1681 ttgcgctcat agaggcgcat ccccttcgca aatccatacg tgaagtagaa gttgggaagg
     1741 tactcgccct ggttctgcgc aatttctggc ctcatgtagt cctggtaccg atactggatc
     1801 cagtcaaatg ccatcttggg gccgttgcca tcgttctcat tgatgtttga ggcgataagt
     1861 tgtccatcaa atccctggtt gtcatcatcc cagtagatcg aaatctgagc attatcccca
     1921 aggtcttcgt catcccaaag gatacgcaac tgctccacca cctcatcact tacgccatca
     1981 ggtttgacaa cagagattgt gggcggaagg ttgtaggtga tcgttccgag ggaatagacc
     2041 acgacaggat cgttgacact gtcaaatatc tcggcgtaaa gatacatcgt ctgcggcggc
     2101 acttgggaca ggttccactc aaacgtgtga agtcccccgt tgttggtgat cggcagagct
     2161 gacgcgatca gggttccgtt cgctccactg ccatcactat catagtagat attcacagtg
     2221 gtcgtcgcgg cattctcggc atcaaaagcc acccaacgaa tagtgaattc cctgctgcca
     2281 ccaagaatat tctccccttc aacccgcgga gagacaaact gcaggaacgg tttcttgttg
     2341 tttgtggcac tgctgatgtc gtgcgagaga gccatcgcat tcacgctgta cgagttgaac
     2401 acgggactac catcggtgat gcggccgagg acgtaataga gcccagccgt gacattggcg
     2461 atgtccttat agatgtaata gtcgtcttca ttgtcctcag acagaccgga gacaatcggg
     2521 atctcgtttc catggtatcc cgtcttgtct gtgtcaagat agaaatcaat ctgagcgtcc
     2581 gaatcccagt ctttgtccgt ccacaaaagg acataaatgt catctacctt ctcgccctga
     2641 gtcttaggca gggcgaccgt catggtcggc gcgtggttgt ccgcaacctt gacgcttcct
     2701 gtgctgtaaa cagagatggc cgggttgaca ctgtcatcaa caacagcgta gacatagtac
     2761 cgccccgctg gtacttcaga tgtatcccag gtataggcgt tggcattgtc gtcaacggcg
     2821 atgttcgaga cgataagagt cccgtcatac cccacggtat cggtatcgta gtaaagcgcg
     2881 atcttcgcgc catccgagag attggtatct ccatcgccat ccgtccaagt cagccgataa
     2941 gtcgcgtcag cagggtcatc ctcaacgacc ggcttggtga aattgaacgt cggtggcctg
     3001 ttgatataaa ccctgccgtt gctgagatcg gagaagacag cattcttgcc atcatccaca
     3061 gaaactcgaa tccgatacct tcccgaactc acatcagtca cactccagac gtacgagcca
     3121 gcaaccccgt cattctcaga cagaccacct acgatgagac tcgggctggt gccgctttca
     3181 cttgtacggt agagactcac cacggcattg tcatcaaggt caatcgcgtt ccattggatc
     3241 tcaagactcg tgctggtaac gacatctgat gcgagagggg tgatcgcgac tgcagcgggc
     3301 tcccctcggt gctgaacgtc aaaacggtag gaagaccagt aatcgcgata ggtcgttctg
     3361 ccgtcgtccg acagtcgagc gaggatgtag tagaggtctt caggcagaag acttgtatcg
     3421 cagaggaaat ggtacggtgc ctggttcaca cgcagcgttc cagtaacgaa gggaatgctt
     3481 ccggagacag ttctcgacgt gttgtagtac agatcaatcg tcgcctccgg agagtcaatg
     3541 tcgaatgcgt tccattggac caagccgcct ttgtcaacca ctacattgga aggagccggt
     3601 tcgagaaact caaaccgagg aatgtgagag gtgaccacaa ctgtcccagc gctgtaactg
     3661 acgacgggaa tgttcacacc atcgtcaata attgcgtaga tgtagtactc gcccgcgggg
     3721 acacgtgtca catcccacgt ataggaatca gagctctgtc ccgtaagcgt gtccgtgatg
     3781 agcgttccgt tctgtcccac attgtccgtg tcgcagtaga gagatatttt cggccttcgg
     3841 gagtcctcgg tatcgtccca ggtaatgtac tggaactgcg cttccaccgt cgcctgtttg
     3901 actgtggcgg gctgcagcaa ctgaatcgag ggtcgtttca gaacttcaag acttcccaaa
     3961 gcaaaatccc gcacctgctg tgtcgcagac tgcagtgaac cgtagacata gtatgtcccc
     4021 gatggaacag aagtcatatc ccaggcatat gtcgccaact gtccatgctg tataagaaga
     4081 tttgaagcgt cctgaatcag gacatcgctt ccgtatgatt gatctgttga gtaatacatt
     4141 gagactgtcg cagaggcgat agtatcccat gcataccact gcaagttcac gctcggagat
     4201 tcaccccaaa gcctctgctg gccagtcttc ggtgcaacca tcgtcagaga aggtctgcca
     4261 tcgcgaacaa tagttagacg tcggctgctt cggctctcgt agtaagtcgt gccatcctgt
     4321 gtaatgcggg cgaccagata gtactgtccc ggagttacat cggatgtgtt ccacgcatag
     4381 taatcctgaa gcgaatcctc agaaagcccc gaaacaatgc tgattggcca tccgtttgca
     4441 gtgtttgtat cgttgtccgc caccaaatcg atccatgcag aggagtcaac atccatgtct
     4501 tcccactcga tcacaaatcc gaaatccacc gtctcatcgg cacttgccag agatttcatg
     4561 gaaatgtacg gaggatgagc ggtgatggtc agaggcccag aactatagga gatgactgcg
     4621 gaattcactc cgtcctctgc aagaccataa atccaatagt ctccaggaag catatctcgt
     4681 gtattccacg cataactgcg tgaacccaga ttcgccttgg caatctcagt gccatccaga
     4741 cctgagtcgt tggtatcata gaaaagtgtc acctgggtgg gatccgggtc gctaccttcc
     4801 caacggatag tgtatgtgtt ctcagctgtg tctccaacac catccggctc ggtgatacgg
     4861 aacgaaggag gacggttcag acgcattgtc tcaaccgcgt agtcggccac atagacattg
     4921 tccccatcct cagcaaccgc gtaaaaatag taggtccccg ctgacaactg actgatgtcc
     4981 caattgtagg atgaaactat accatcttgc agggtcagac catcagcgat gagcgttcca
     5041 ttgactccgg aattgttatt gtcccagtag agactgacct tcacattcgt gctgtcggga
     5101 tcatatccga cccagcggat tgtatagagt gaagcgtctt ccttgagatc aactccatca
     5161 gagccgggct caagaacaac aatggtcgga ggtgaattgt gtgacacgac aacacgccct
     5221 gtgctgtagt tgtcgtagta cgtcgcaccg ccatcatccg taattcgtcc caatatgtag
     5281 taactgccgc caggaacact cgacgaatcc caaagcgtgt aatcaagaag atcatcttct
     5341 gagattccgc tggcaatgag tatctcgtca ccatcaaagc ccgcgttgtc gtcatcggca
     5401 tacagactga tggaagcgtt gctgtctcgg tcactgtcag tccaggtaat ataataggac
     5461 ttgtccgcag gccagttgcc tgtcgcagga tccacgattg tgatcttcgg cttattctcc
     5521 agtaccgtaa acggccctgg agaatagaca tattgaggag gattcacacc atcatcgatc
     5581 ttgaggtaaa tgtaatactg accggaaggc atggactgaa catcaaaaaa gtactcatcg
     5641 ggaatcccct caaagggaat cccacaaagg aaatactcga tgaaaccggc agccgtcatt
     5701 gaaggcgaac caccccctcg gcctgaatag ccaaactggc cgttcgtcga aggattcgta
     5761 gtgacgttct ggctgtactc caaccagtcc aggaaaccaa ctccataggg atcgtctgga
     5821 tggtggctgt cccacgccca ttctggaaca acggcattga acggacattg catcgcgccc
     5881 cacaagccca acgcagccca ctgggaaccg gaattgtccg tcccagcacg gaatccatac
     5941 tgccaaccac caatggaggt ctgagcagca acaatccagt cggccaaatc ctgaatgtag
     6001 ccattgtaga tcgtcgtttc acgatccgag gtcaacgcca tcaacgcgat acccgtcttg
     6061 taagggtcaa cgctcgtgta ccggtacccg actccatctc tgtctccgtc aaacagtgga
     6121 tagccgctca ggtcactgta gacccccata ccgcaaaggg ctcgaaatcc cgccgcgaga
     6181 tactcggcat agatgctttg atcgtagtcg tccatcggca gaaagtcatt gtcaataaaa
     6241 gcaagcagaa ccagagcggt cgccgagtca tcatgacccg ccgctctcca cattccagag
     6301 cttttctcct gctccagata gaggaagcgc agcgcatcct gaatcatccc cttgcgacgg
     6361 gactcagttc catccgcaac cacgttaatc ttcacagtgt ctgaatctga actgcccttg
     6421 cggtcaacca ctgtcagggt cgcgtaatag gttccaatgg atttgtagtc atgctccact
     6481 gcaatgtaga gcgggtctac gaccgcaccg aatgcgggct gagacccatc tccaaaatcc
     6541 cagatgtaag aaccaccctc agatccgaca gcccagtgaa catttcccca gacctgcaca
     6601 tttccgcgac cagcccacgt cgtgtactca ggcatgacat tgacccgaat tgtgctctta
     6661 accacgtccg gaccttcatt ctgccaaagc gttcgtggct catatctggc ttcaccaccg
     6721 ttgatatctg ttgcggcaaa acgatagcga gcacgattgc ccatcgggag tttggtcttg
     6781 taactgtaga caacgccact ctggaaattg gtggcagcag ggttaacctt ggtcatctga
     6841 tatccgccct cgcggaagtc cacaccctcg tcgttctcat ccccatcgcc attgatgtca
     6901 atgtagactt tggggaaacc gtctgcgggc ggattgttgt ccggatccgc ataggtgaca
     6961 cggtactcaa aaagcgtatc ttgatagccg atcatcggct cgacaccatt gtgatggtat
     7021 ggatatgagg cgtaggggag ataccagtag ccctgatagt ctcctgacca ggcaagagtt
     7081 ggacgcctgt ttccgatata gaggaatgtg gaggcaatgt tgttcacctc gctgtactca
     7141 tcaatgtcgt catcgggatc aagtcgaaca aaaatgctat ggctgccttc atccgtcgcg
     7201 aaggtcaccc agtcataaac catttccacc acacttccag cggcaacatt cgtaatcaag
     7261 ttgcttccca actgtcgact cagaggattg agcggattgc cgtcaaatat ctgaaccacc
     7321 gcgccgtttg cgggaatcgt tcccacattg cgaaccgatg ctgtgatctt cactgtgttt
     7381 ccgggaagtg gatgctccgg atcaaaatgg atatccgcag cggagataac aagatcagga
     7441 acattgggga tcagaacaaa gatgttcttg accgcccggt tgttcatttc attgatctca
     7501 atgatctcgt catcgggatc agccagacag ttgatgcggt gaag
//
LOCUS       DAOQUC010000013         7527 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_10795_length_7527_cov_3.639989, whole genome shotgun sequence.
ACCESSION   DAOQUC010000013 DAOQUC010000000
VERSION     DAOQUC010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7527
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10795_length_7527_cov_3.639989"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(102..3842)
                     /locus_tag="PKH07_00450"
     CDS             complement(102..3842)
                     /locus_tag="PKH07_00450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33452.1"
                     /translation="MKSLLWILFLLLSASSRAFAFHGHQVQESGLLLRIEELPRITQY
                     DVPVAVRVFLENTSDAPVSGTVTVKDLVDEWRVKGKPSRHFQLSQGASCELHFSILVG
                     KGALSALYPIHAYASFKKELEQFSVHAVRVVQAEFPEADRTHDVAPQLRPIVIPDQGS
                     LSLLRSKFFRVAWRYENQPLRYKSVGWDGRDAQSDAMFWTSSAVERGGTRTAIAMHPP
                     YRGGTGSVFCDYQLRLPKITPLVLDYANAIRTHHGTEPPSDGVTFRVWAITGETEHLL
                     DEHHTDSKVWVPRQADLTRFAGQEIVLRLESHPGADLNTVCDLSYWADVCVTAGAADL
                     LPSETAPFSERAEAISEEAVRTARSTSGTATESGILKKQESTNRFSFAANAKDGLIVF
                     VIEPGQRGLLDGCLAVAAPNGASVVFNGLQIVVGDQPLQQGLSDVLLKAFNLRRSASR
                     LVADHLLRDPQGDFRLKIVLWLEGGGLRLRTECSRHISDIALGQTNGAAKKVYWGHGY
                     CLVEPEPFSMSFGGHTLSTSHVGFDFESGLSVLQAFDNPPTRLDVSTREGIHALHSTG
                     NATLTLVAGVHGAMNCALQYRNLYDRAAASRVINLAGRVGFDFWGGHYADIEQEMKRM
                     AQYGCTDAVLTIHNWQRWGYDYRLPDVFPPNPRMGTLEEIQRLGKTCREIGILWGLHD
                     NYIDFYPDATGYTYDQITFTRHGAPVKAWLNEGMDAQSYQFRPDRIMPYVKRNLRLVK
                     KHLQPTHYFLDVFASIGPFEYYDRSGKFHSNVETRDCHGQAFAWIRKCLGDNAPTTSE
                     AGHDQLTGWLDGSDCQFLRITPQAERFGLHLPCQDWERVPWFDAVLHDKLILHGVGYS
                     DRFQANLSRREHGITSDDYISAEVLTGHPMMTDVGCIGAQAVRKYWLLHGIGRDLAMQ
                     RIERVEFADQDIHRQTILWGNGTTVWVNRSQQDWIVEGHVLPPCGYYVRGNNVEGAVE
                     RIGDVVVERSIGADGWFFNSRNSKDPNALAITPAVESFEYIGQRQFKLTLKWDVQQIV
                     PAGLIPFVHFLPQGGSRTGKIAFQGDHKPEISTEKWTGVVLSGQNRIIQVPSAIEETC
                     FDVAVGLFKPGINVRYSLNGEEVENCAYRVGVLHLDIKNQEINVIRFEPTPAPQATQE
                     RDERSPNASVDFGTVITDGALRITPQQDRFVVTPLPDSPPFSADFHLASILSSEVPEN
                     VLVETKDMEGRTLHAEMTPVSNGRLSVHHQGGQVQYQVSRVD"
     gene            complement(3889..4947)
                     /locus_tag="PKH07_00455"
     CDS             complement(3889..4947)
                     /locus_tag="PKH07_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007326986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HNT33453.1"
                     /translation="MSRTVMQSLSKGVVIPACPLALDVERRFDERHQVALFRCYGDAG
                     AGGVAVGVHTTQFEIREPRFGLFEPLLRLASSELSAVSDRVGRDLIKISGVCGKTDQA
                     VREASLAASLGYDIGLLSLAALKDATDDELIAHCSRIAEIIPIMGFYLQPAVGGRVLS
                     YSFWRRFAEIENAIAIKISPFNRYQTFDVVRAVADAGREQPITLYTGNDDNIVMDLLT
                     PYVFRTKEVFTTVRIKGGLLGQWSVWTRCAVQMLNDIHALVDRQSVVPAEWLTKNAEL
                     TDANGALFDVANRFAGCIAGINEVLRRQGLLASNCCLSEHERLSPGQADEIDRVCKAY
                     PRLTDDDFVAEHLDMWLA"
     gene            complement(4944..6002)
                     /locus_tag="PKH07_00460"
     CDS             complement(4944..6002)
                     /locus_tag="PKH07_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008666709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epimerase"
                     /protein_id="HNT33454.1"
                     /translation="MASIPDLRGSEETLEQLLSIPYPETVEMMKRLEGDLLILGVGGK
                     IGPSLAATAANACAKAGVQKRIIGVDKVLTAEVTERLTRLGVETQLCDLSEYEQVNDL
                     PRVRNVIFMAGRKFGQVGSEPLTWLMNAVIPGNVGQAFRGSRIVVFSTGCVYELVSTD
                     GIGSLETDRPKPVGEYANSCLGRERVFEYYCSTFKTPVLLFRLNYAVDLRYGVIVEIA
                     KQVYDGKPVDITVNAANVIWQGDAVNRALLCLEHTSCPAAILNITGRQTLRVDAVAQQ
                     FGQIFRKTPSFCGSNSGKAYLSNADRSFRLFGLPRVTETQMIQMVAEWMKTGGAILDK
                     PTHFNVTDGQFLDGGGKA"
     gene            6196..6672
                     /gene="nrdR"
                     /locus_tag="PKH07_00465"
     CDS             6196..6672
                     /gene="nrdR"
                     /locus_tag="PKH07_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005330544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HNT33455.1"
                     /translation="MKCPYCGEDDDRVVDSRSMNDGLAIRRRRECLECQKRFTTYERA
                     EEIELSIIKRDQRREPYERKKITDGIRKACQKRPVSEEKIQEIADCIERELHSDENKE
                     VPSTRIGELIMEELRKTDQVAYVRFASVYRQFKDANQFMKELQGILEIEGKPGRQR"
     gene            6669..>7527
                     /gene="amrS"
                     /locus_tag="PKH07_00470"
     CDS             6669..>7527
                     /gene="amrS"
                     /locus_tag="PKH07_00470"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HNT33456.1"
                     /translation="MSHKARWYRVHNADTIACLLCPRACLLKEGEYGSCQVRHVEHFE
                     LVTDTYGKITAIANDPIEKKPLYHFYPGSNILSIGTFGCNFACTFCQNWRISQGKPPT
                     QDLSVRELVRAAGQNGSIGVAFTYNEPSIWYEYIMDAAPQLRQKGYKVVLVTNGYIQP
                     EPLEELLPFVDAMNIDLKSSRDDFYKHFCGGRLKPVLETIRHASTKTHVEVTHLIVTG
                     QNDKKEQILDLVGLVADIDKDIPLHFSRYFPQHRYTQEATPSEFLHWAWDAAREKLTS
                     VYLGNLQSEK"
BASE COUNT         1836 a         2067 c         1963 g         1661 t
ORIGIN      
        1 aggctctgcg acgctgccga agcgggaaca gccagacagc gggcttcggt agcgtcgctc
       61 cgagcccgtc ctgagcgaag tagaagggct cccaatgaca ctcagtccac acgcgaaacc
      121 tgatactgaa cctgaccgcc ctgatggtgc acgctgagtc ttccattgct gactggcgtc
      181 atttcggcgt gaagcgtcct gccttccatg tctttggtct ctacaaggac gttctcaggg
      241 acttccgaag aaagaatact cgccaaatgg aaatcagcag agaacggtgg gctgtcggga
      301 agtggtgtga cgacaaatct atcttgttgt ggcgtgatcc gaagcgctcc atcggtaatc
      361 accgtcccaa aatcaacgga cgcattcgga gagcgctcgt ctcgctcttg agtcgcttgt
      421 ggagcaggtg ttggctcgaa tcggatcacg ttgatctcct gattcttaat gtccagatgc
      481 agaacgccca cgcgataggc acagttttcg acttcctccc cgttcaggga atatcgcaca
      541 ttgattccag gtttgaacag gccgacagca acgtcaaaac aggtttcctc aatagcggag
      601 ggaacctgga tgatcctgtt ctgaccggaa agaacaacgc ctgtccattt ttcagttgat
      661 atctcaggct tgtgatcgcc ctgaaacgcg attttgcctg ttctggaacc tccctgcgga
      721 agaaagtgga cgaagggaat caaacctgca ggaacgattt gctgaacatc ccacttgaga
      781 gtgagtttga actgcctctg tccaatgtac tcgaacgact caaccgctgg agtaatcgca
      841 agtgcgttgg gatctttact gtttcgggaa ttgaagaacc atccgtctgc gccgatgctt
      901 ctctcaacga cgacatcgcc gattctctca acggcacctt cgacattgtt gcctcgaacg
      961 tagtagccgc acggtgggag aacatggcct tcgacaatcc agtcttgctg agagcgattc
     1021 acccagacgg tcgtgccgtt gccccagagg atcgtctgtc ggtggatatc ttgatcggca
     1081 aattcaacac gttcaattct ctgcatcgcc aaatctcggc caatgccatg aagtaaccag
     1141 tatttgcgaa ccgcctgagc gccaatacac ccgacgtcag tcatcatggg atgtccggtc
     1201 agaacttcag cgctgatgta gtcatcgctg gtgattccat gctcacgtcg cgacaggttc
     1261 gcctgaaatc gatccgagta gcccactcca tgcaggatca acttgtcatg caacacagcg
     1321 tcaaaccagg gaactcgctc ccagtcctgg catggaagat gaagaccgaa gcgttctgcc
     1381 tgcggtgtga ttcgcaggaa ctgacagtcg gaaccgtcaa gccagcctgt cagttgatcg
     1441 tgtccggctt ccgaagtggt gggcgcattg tcaccaaggc attttcgaat ccaggcaaag
     1501 gcctgaccat ggcaatcccg agtctcgacg ttggagtgga actttcctga acggtcgtag
     1561 tactcaaaag gccctatgga cgcaaagaca tcaagaaagt aatgcgtcgg ttgaagatgc
     1621 ttcttcacga gcctcaggtt ccttttgacg taaggcatga ttcgatctgg gcgaaactgg
     1681 taactttggg catccatgcc ttcattcaac cacgccttca ctggcgcacc atggcgggtg
     1741 aaagtgatct ggtcgtacgt gtagccagtc gcgtcagggt agaagtcaat gtagttgtca
     1801 tgaagacccc agaggattcc aatttcgcgg caggtctttc ccaggcgctg tatctcttcg
     1861 agtgtaccca tgcgcggatt tggcgggaag acatccggta atcggtagtc atatccccaa
     1921 cgctgccagt tgtgtatggt cagcacagca tccgtacaac catactgcgc cattcgtttc
     1981 atttcctgct caatgtcagc atagtgacca ccccagaaat caaagccaac gcgaccagca
     2041 agattgatga ctctggaggc agcagcacgg tcatagagat tgcggtattg aagagcacaa
     2101 ttcatcgctc cgtgaacacc agcaaccaga gtcagggtcg cattcccggt ggagtgcagc
     2161 gcgtggattc cttctctggt agacacatcg agacgagtcg gtggattgtc gaacgcctgc
     2221 agtaccgaaa gccccgattc gaaatcaaac ccgacgtggc ttgtggaaag ggtgtgcccg
     2281 ccaaaggaca tgctgaaagg ctctggctca acgagacaat aaccatgtcc ccaatacact
     2341 ttctttgcag cgccattggt ctgtcccagc gcaatatctg aaatgtgtcg tgaacactct
     2401 gtgcgaaggc gcaacccacc accttccaac cacagaacga tcttcaggcg aaaatcaccc
     2461 tgggggtcgc ggagcagatg atcggcaaca aggcgactgg cgctgcgccg cagattgaag
     2521 gctttcaaca gcacatctga caatccctgt tgcagaggct gatccccaac gactatctgc
     2581 aatccattga agacaaccga tgctccattt ggtgcggcca ctgcgagaca accatcaaga
     2641 agtccccttt gtcctggttc aatgacaaaa acgatcagac catccttggc attcgctgca
     2701 aatgagaacc gattggttga ttcctgtttc ttgagaatgc ctgattcggt cgctgtcccc
     2761 gatgtggaac gtgcagtccg aacggcctct tctgaaattg cctctgcgcg ttcagagaag
     2821 ggtgctgttt cggaaggtaa aagatcagcc gctccggcag tcacgcagac atccgcccaa
     2881 taggacagat cgcagaccgt gttgagatcc gcccctggat ggctctccaa tcgcagcacg
     2941 atctcctgac cggcgaatct cgtcagatcg gcttgtcgag gcacccacac cttggaatcg
     3001 gtatggtgct catcgagcag gtgctcagtc tctcccgtga tcgcccaaac gcgaaaagtg
     3061 acaccatcac tcggcggttc agtcccatgg tgggttcgta tggcatttgc atagtcaaga
     3121 acgagggggg ttatctttgg caatcggagc tggtaatcac aaaagactga accagtgcca
     3181 ccccgatatg gcggatgcat ggcaatggca gttcgagtcc cacctctctc aacggcggaa
     3241 gaagtccaaa acatggcatc cgactgagca tccctgccat cccaacccac agacttgtag
     3301 cgcaggggct ggttttcata tcgccaggca acacggaaaa acttagagcg aagcaatgaa
     3361 aggcttcctt gatcaggaat gacgatcggt cgcagctggg gagcaacgtc atgggttctg
     3421 tccgcttcgg gaaactccgc ttgaacaacg cgaacggcgt ggacagaaaa ttgttctaat
     3481 tcctttttga acgacgcata ggcatggatc ggatacaggg cactcaatgc ccctttgccg
     3541 acgagaatgc tgaaatgcag ttcacacgag gctccctgtg agagttgaaa gtgacgagac
     3601 ggctttccct tcactctcca ttcatccacc aagtccttga cggtcaccgt ccctgaaaca
     3661 ggagcgtcag aagtgttctc aagaaacaca cggacagcaa caggcacatc atattgcgtg
     3721 atgcgaggaa gttcctcaat cctcaggagc aggccgcttt cctgaacttg atgaccgtgg
     3781 aaggcaaagg ctcgcgagga agcagacaac aacagaaaga ggatccacag caaggatttc
     3841 atgatggcat tctcccggtt cacaggctat ccgatgcagt gtctcaggct aggcaagcca
     3901 catgtcaagg tgctcggcaa cgaaatcatc gtcggtcaac cgcggatagg ctttgcacac
     3961 tcggtcaatt tcgtccgctt gacccggaga gaggcgttca tgctcactca gacagcagtt
     4021 gctcgccagc agtccctgcc ttcgaagcac ttcgttgatg cctgcaatgc atccggcaaa
     4081 gcggttggca acatcaaaca gcgccccatt ggcgtcggtc agctcggcgt tcttcgtcag
     4141 ccactccgca ggaacgacag attgtctgtc aaccaatgcg tgaatgtcgt tgagcatttg
     4201 gacagcacat ctcgtccaca cactccattg gccgagcagt ccccccttga ttcgaacggt
     4261 cgtgaaaacc tcctttgtcc gaaagacata gggagtcagc agatccatga cgatgttgtc
     4321 gtcgttgccg gtgtagaggg tgatcggctg ctctctgccg gcgtcagcaa ctgcgcggac
     4381 gacatcgaat gtttggtagc gattgaaggg tgagatcttg atcgcgatgg cgttctcgat
     4441 ttcagcaaac cttctccaaa aggaataaga aagcactctt cccccaacgg caggttgcag
     4501 gtagaaaccc atgatcggga tgatctcggc aatccggctg caatgtgcga tgagttcatc
     4561 atcggtggca tccttcaagg cagccaggct cagaaggccg atgtcgtatc ccaaagatgc
     4621 ggccaaggat gcttctcgaa cagcctgatc ggtcttgcca cagactcccg agattttgat
     4681 caaatccctg cccactcgat cgctgactgc actcaactcg gaagaggcga gtcgcaaaag
     4741 cggttcgaaa aggccaaagc gtggttctcg aatttcaaac tgcgtggtgt gcacaccgac
     4801 ggcaacacca ccggcaccgg catccccgta gcagcggaac aacgccacct gatgacgttc
     4861 atcaaaacgc cgctctacgt ccaacgccag aggacaggca ggaatcacca cacccttgct
     4921 gagcgattgc atcacagtcc ggctcatgct ttccccccac cgtcgagaaa ctgtccgtcc
     4981 gtcacattga aatgagtcgg tttgtccaga atcgcgccac cggttttcat ccactctgcc
     5041 accatctgga tcatctgcgt ctccgttact cggggcagac cgaaaagccg gaaagaacgg
     5101 tcggcattgc tgagataggc ctttccggag ttggagccgc agaacgaagg ggtctttctg
     5161 aagatttggc caaactgctg cgcgacggca tccactctca gtgtctgtcg tccggtgatg
     5221 ttcagaattg ccgccgggca tgatgtgtgc tccaaacaca acagagccct gttgacggca
     5281 tccccctgcc agatgacatt ggccgcgttg actgtgatat caaccggctt tccatcatag
     5341 acctgcttgg cgatttccac gataacacca taccgcagat caacggcata attcagacga
     5401 aacagaagca caggcgtctt gaaagtggaa cagtaatact cgaagacccg ttcgcgtccc
     5461 agacaggagt tggcatattc ccccaccggt ttgggtcggt ctgtttccaa ggaaccgatg
     5521 ccgtcggtgg agaccaattc ataaacacaa ccagtcgaaa agaccacgat gcgactgccg
     5581 cgaaaagcct gaccgacgtt tccgggaatg accgcgttca tcaaccaggt cagcggttcg
     5641 gaaccgacct gcccgaattt tcgccccgcc ataaaaatga cgttgcgaac tcttggaagg
     5701 tcgttaacct gttcatattc cgaaagatca cacagctgag tctcaacgcc aaggcgcgtc
     5761 aaccgttcgg tgacctcggc ggtcagaacc ttgtccacac cgatgatcct cttctgaaca
     5821 cccgctttgg cgcaggcatt ggctgctgtc gcggccaacg agggtccaat cttcccgcca
     5881 accccaagaa tcagcagatc cccctccagc cgtttcatca tctccacggt ttccgggtag
     5941 ggtatggaca acagctgctc caatgtctcc tcggagcctc tcaggtcggg aatgctcgcc
     6001 atttgaatct cctttgtgaa aaaactgcac gggaagatta tatccgtact cactcagctg
     6061 gcaactcttg tttttgtctc cctcgactcg atctggattg cagtctactg aatcttgtac
     6121 tataaaatgc agaacgcact aattattgtg gtgcgtcttg gagcagatct acgtggcgga
     6181 gcaagggagg tttctatgaa atgtccttac tgcggtgaag atgacgaccg ggtcgtggat
     6241 tcgcgttcca tgaacgacgg ccttgccatc cggcgtcgtc gggagtgtct ggagtgccag
     6301 aagcgcttca caacctatga acgcgcggag gagattgaac tctccattat caagcgtgat
     6361 cagaggcgtg agccgtacga acgcaagaag atcacagacg gcatccgaaa ggcctgtcag
     6421 aagcggcctg tcagcgagga aaagattcag gaaatcgccg attgcatcga gcgggaactg
     6481 cattccgatg agaacaagga agttccctcg actcgtatcg gggaactgat catggaagaa
     6541 ctccgcaaga ccgatcaggt tgcctacgtt cgttttgctt cggtctatcg gcagttcaag
     6601 gacgccaatc agtttatgaa ggaactgcaa ggcattttgg agattgaagg gaaacctggc
     6661 agacaacgat gagtcacaag gcacgatggt atcgcgttca caatgccgac acgatcgcgt
     6721 gtctgttgtg tccgcgtgcc tgtcttctca aggagggtga gtacggaagt tgtcaggtgc
     6781 gtcatgtgga gcactttgaa cttgtcacgg atacctatgg caagattacg gccattgcca
     6841 acgatcccat cgagaagaaa ccgctctatc atttctatcc gggtagcaat atcctctcca
     6901 tcggaacgtt tggatgcaac ttcgcatgca ccttctgcca gaattggcgc atctcccaag
     6961 gcaaaccacc cacgcaggat ctctccgtgc gagagttggt tcgagcggcg ggacaaaacg
     7021 gctcgatagg tgttgccttt acctacaatg aaccttcgat ctggtatgag tacatcatgg
     7081 acgcggcacc gcagttgaga cagaaaggtt acaaggtagt gctggtgacc aatggttaca
     7141 ttcaaccgga gccgctggaa gaactgcttc cttttgtgga tgcaatgaac attgatctga
     7201 agtcttctcg agatgatttc tacaaacact tctgcggcgg gaggctgaaa cccgttttgg
     7261 agaccattcg tcatgccagc accaagacac atgtcgaagt cactcatctc attgtcacgg
     7321 gacaaaacga caagaaagag cagattctgg acttggtcgg actcgtggca gacattgaca
     7381 aggacattcc actccacttc tcccgttatt ttcctcagca ccgctacaca caggaagcca
     7441 caccgtcgga gttcctgcat tgggcttggg atgcggctcg ggagaaactg acttcggttt
     7501 acctcggaaa tcttcagagt gaaaagg
//
LOCUS       DAOQUC010000014         7514 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_10808_length_7514_cov_3.560263, whole genome shotgun sequence.
ACCESSION   DAOQUC010000014 DAOQUC010000000
VERSION     DAOQUC010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7514
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10808_length_7514_cov_3.560263"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            122..955
                     /locus_tag="PKH07_00475"
     CDS             122..955
                     /locus_tag="PKH07_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003540368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HNT33457.1"
                     /translation="MEINKMKQLEEKARQIRIDSIQMLTEAGSGHPGGSLSVADLVAA
                     LMFDVMKHDPKRPDWEDRDRFVMSKGHCIPAWYSALAQAGYIDRNLLPTLRKLGSPLQ
                     GHPDRVLLPCLEASTGSLGQGLSIAVGMALASKMKGNAFRVFCMLGDGEIQEGQVWEA
                     AMAAAKFKLDTLVAIVDHNGLQIDGFVEQVMPLEPLADKWKAFNWHVISIDGHEFGQI
                     LPAFERASDTKGKPTVILAKTIKGKGVSFMENLCDWHGVAPNREQEAQALAELGVTKS
                     K"
     gene            967..1911
                     /locus_tag="PKH07_00480"
     CDS             967..1911
                     /locus_tag="PKH07_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012646457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HNT33458.1"
                     /translation="MAAKATRQTFGETLAEIGATHPNVVVMDADLSKSTMSKLFAAKY
                     PDRFFELGIAESNMVGAAAGMALCGKIPFICSFACFLTGRYETIRISVAYSQVPVRIV
                     GTHAGIGIGEDGNSQMGLEDLTIMRALPHMTVIQPADDIETRQAVQLLATNAVPGPAY
                     LRLTRQKLEAVNKSDYRFQLGKGVVLRDGSDMTILATGGVVHNSLKAAELLTTQGINA
                     RVVNIHTVKPIDADLIEKCARETKAFLTVEDHNIVGGMGGAVAEVLADRGPFGIRMLR
                     WGIQDRFGESGSPEELYQAYGLDVDGILAKAKEVYARI"
     gene            2004..3194
                     /locus_tag="PKH07_00485"
     CDS             2004..3194
                     /locus_tag="PKH07_00485"
                     /EC_number="2.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015756208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine transaminase"
                     /protein_id="HNT33459.1"
                     /translation="MTNKQMAEASKKYLFNNYGERDMALVRGQGARVWDADGNEYIDM
                     LAGIAVCNVGHCHPKVVEAAQRQLTQLIHVSNLFNIEPQIQLAQRLVDNSFADKVFFC
                     NSGAEAVEGALKLARRYGTSQRGSECVEFIAAENSFHGRTFGAISATGQEKYRKGFEP
                     LLPGVRFVPFNEIKAFDDAFSERTCGVILEPIQGEGGVMPACREYLQHVRSRCSETNT
                     VLIFDEIQCGMGRTGRLFAHEHYGIEPDVMTLAKALGGGLPVGALLARDRFADVLGPG
                     THASTFGGNPVVMAAALAVLELLIEGGLLERCAQLGAYFKDRLSQLAEHSPKVKEVRG
                     AGLMLGVVLDSPAKPVMMALREKGILCATALDTVVRFVPPFVIEKADIDRTVDALGSI
                     LKSL"
     gene            3194..4351
                     /locus_tag="PKH07_00490"
     CDS             3194..4351
                     /locus_tag="PKH07_00490"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HNT33460.1"
                     /translation="MKIGIDISETTGEKTGVGYYTTHLVEALAAVDRVNQYTLYPVFF
                     DNFPPTYDRATCPEQDNFSLFGKSLSRRKLTHLWHKSGMPKEQLFGQQDVFHSTTFSS
                     PELTESSLIVTLYDLSFWTHPQCHRPINRRYCAERCCLAARRASRLIAISEATKRDIL
                     KYLHVPEERVVVTPLAAHPRFRPVEDAWCKHQVLMKYGLLTDYIFSLGSLEPRKNIAT
                     LIRAYSALPDRLRRTTHLAIAGAKGWKNSNLFALIEQLSLGDCVKVLGYVPDDDLPAL
                     YSSASVFVYPSLYEGFGLPALEAMACGAPVIVSNTSSLPEVVGTAGIQIEPTDVEALT
                     QAILRVLLQPDLSEELRRKGIEQAHRFSWQRTAELTLQAYVDAAAEKNRRK"
     gene            4603..6030
                     /locus_tag="PKH07_00495"
     CDS             4603..6030
                     /locus_tag="PKH07_00495"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HNT33461.1"
                     /translation="MARKNDRRALKKTNGAKHPSLFEEHPAENVHETVNEFGETLVRV
                     DGDIPVDLPIAEGDRFLFISYDQSILTHGLHKYPAKFFPELPRWLIRRFSKKGQLVLD
                     PFAGSGTTNLESLLLHRHSVGIDVDPFSRFLARVKTTALDKEDVLAAQDEVLRRCVQF
                     APEKVRPTDVPNFPYRDSWFDPEITLELTYLRKTIDDLNCSQETADFLRICLSSIVRS
                     VSNADDNCTRTVIRKRLNKIVLPSDALRRFAETVMLNVPRVIAFSERRPENVRVEFPP
                     DMDSRSMRFPDGTFDLAVTSPPYANAVDYPRTHQLEIYWLGLAEGSLTPLKRKHVGTE
                     SVSAADYTYLHQTGHSRADVVLSQIFEKDPRRSYIAFKYLDDMRQNLREVFRVLKRGA
                     AYIVVVGNNRIRGELFENWKYIMDLAEEVGFHVENYFGSEIIKHFIKVPREERINTDW
                     ILILRKPEVGCSAPSGSALPERKKK"
     gene            6027..6860
                     /locus_tag="PKH07_00500"
     CDS             6027..6860
                     /locus_tag="PKH07_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HNT33462.1"
                     /translation="MRLGGPVFDKYDSPGEWVRAVRSLGYRAAYCPLGLDAPQDVICA
                     YEHAAEQADIVIAEVGAWSNPLSPDDDVRKQAVEKCKRSLALADAIGARCCVNIAGSR
                     GAKWDGPCKEDLTDETFDAIVEVVRDILDDVQPQRAFYTLETMPCMYPDSTDAYIDLI
                     RAIDRKQFAVHFDPVNLVCSPRRYFNNGDLIRDFVARLGSHIRSCHAKDILLQDRLTV
                     HLDEVRPGQGALDYSALLREVSHLDKDVPVMLEHLSSADEYKAAAEYIRSVAAATGGS
                     L"
     gene            6857..>7514
                     /locus_tag="PKH07_00505"
     CDS             6857..>7514
                     /locus_tag="PKH07_00505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HNT33463.1"
                     /translation="MNHHAIVITAKEQADLLPIEEPAALKTHEVRGNTLVTLISPGTE
                     IAWSYLGVQQDHAHAFPKRLGYAAVLRAEQVGSEVHDLEPGTSLLCMGNHQSYQQTEA
                     LAAVPIPPELPAHEAVLARLMGVTMTTLMTTSARPGDLVVVTGAGPVGYLGAHLFALS
                     GYDVCVVEPNERRRAGVQRSGITTVHPTMPLEDKEIKGQVALVLECSGHEQAALDGAR
                     I"
BASE COUNT         1726 a         1973 c         2119 g         1696 t
ORIGIN      
        1 gaaatttcat gatttcgggg ttgactccgg aacttgaaaa atgacttcag ttctaggttg
       61 cacccggtgg gatgatcccc tatcattctc ggacttcttc aaaaaggaga gtttaccggt
      121 gttggagatt aacaagatga agcagttgga ggagaaagcc agacagattc ggatcgacag
      181 cattcagatg ctgaccgagg ccggttccgg ccatccagga ggctcgttgt ctgtcgcgga
      241 tctggtcgca gcgctgatgt ttgacgtcat gaaacacgat cccaagcgac ccgactggga
      301 agaccgggat cggtttgtca tgtccaaggg gcactgtatt cctgcctggt atagtgcgct
      361 ggcacaggcc ggatatattg accgaaacct tctcccaacg ctcagaaaac tgggcagccc
      421 gctgcaggga catcccgacc gtgtcctgct tccctgtctg gaggcgtcga cgggctcttt
      481 gggacaaggt ctttccatag cagtcggtat ggcacttgcc agcaagatga aaggaaatgc
      541 gtttcgggtc ttttgcatgt tgggtgacgg ggaaatccag gaaggccaag tctgggaagc
      601 agcaatggcc gcggcgaagt tcaagttgga tacgcttgtc gccattgtgg atcacaacgg
      661 cctgcagatt gacggctttg tcgagcaggt gatgccgctt gagccactgg ccgacaaatg
      721 gaaagcgttc aactggcacg tcatctcgat agacggccac gaattcggtc agattcttcc
      781 cgcatttgaa cgggcatcag ataccaaagg aaagccaaca gtcattcttg ccaagaccat
      841 caaaggcaaa ggggtttcct tcatggagaa tctttgtgac tggcacggtg tggcacccaa
      901 ccgggagcag gaagcacagg ctctcgcgga actgggtgtg acgaaatcta agtaagggag
      961 tcagaaatgg cagcgaaagc aacacgccag acttttggcg aaacattggc ggaaattgga
     1021 gcaacgcatc cgaatgtggt cgtcatggat gcagacctga gcaagtcaac catgtccaaa
     1081 ctttttgcgg cgaaatatcc cgaccgtttt tttgagttgg gcatcgcgga atccaacatg
     1141 gtgggcgccg ccgcaggcat ggcactgtgt ggcaagattc cattcatctg cagttttgcc
     1201 tgctttctca cggggcgcta cgagacgatc cggatctccg tcgcgtattc ccaggttccc
     1261 gttcggattg ttggaaccca tgcaggcatt ggaatcggtg aagacggcaa ctcccagatg
     1321 ggtttggaag acctgaccat catgagagcg ctgcctcaca tgactgtgat ccagcccgcc
     1381 gacgacatag agactcggca agccgttcag ttgcttgcca ccaatgcagt accggggccg
     1441 gcatatctgc gcctgacccg ccagaaactt gaagcggtca acaagagcga ttaccggttc
     1501 caactcggta agggcgtcgt cctgagagac ggttcggaca tgactatcct cgccacaggg
     1561 ggcgtggttc acaacagttt gaaggcagcg gaactgctca ccacacaggg catcaatgcc
     1621 agagtagtca acatccacac ggtcaaaccg attgacgctg acttgatcga gaagtgtgcc
     1681 cgtgagacca aagcgttcct cacagtcgaa gatcacaaca tcgtcggcgg catgggtggc
     1741 gctgtggcgg aagttcttgc cgatcgaggc ccctttggca tcaggatgct ccggtggggc
     1801 atccaagatc gctttggaga gtccggatcg cccgaggaac tctatcaggc ttatggtctg
     1861 gacgtagacg gtatcctcgc gaaggcaaag gaagtctacg ctcggattta gccgttcatt
     1921 cggtctgcaa agaatagtct catgcacggt ccgggaacaa cccagaaagg tatgttttca
     1981 caggaaccca ggagtcaaga atgatgacaa acaaacaaat ggctgaggca tcgaagaaat
     2041 acctcttcaa caactatggc gaacgcgata tggcactggt tcgcgggcag ggcgcccgag
     2101 tttgggacgc ggatggtaat gagtacattg acatgcttgc tggcattgcc gtctgcaacg
     2161 tgggacactg tcatccgaag gtcgtcgaag cggcacagag acaactcacc cagttgattc
     2221 acgtttccaa cctattcaac attgagccgc agattcagct ggcgcaaagg cttgttgaca
     2281 acagttttgc tgacaaagtc tttttctgca acagtggcgc agaagccgtc gagggcgctc
     2341 tgaaactggc aaggcggtat ggaacttccc aacgcggttc ggaatgtgtt gagttcattg
     2401 ccgcggagaa ttcctttcat ggacgcactt ttggagccat ctcggcaacg ggacaggaga
     2461 agtatcggaa gggatttgag ccgcttctcc ctggcgttcg gtttgttccc ttcaacgaga
     2521 tcaaggcgtt cgatgatgca ttctcagaac gaacgtgtgg cgtcatcctg gagccgattc
     2581 agggcgaagg tggcgtgatg cccgcttgca gagagtatct ccagcatgtt cgcagccgct
     2641 gttcggaaac caacaccgta ctgattttcg acgagatcca atgtggaatg ggacgcaccg
     2701 gcaggctttt cgcccatgag cattatggaa tcgagcccga cgtcatgaca ttggcgaagg
     2761 ctttgggcgg cgggctgcct gttggcgctc tgttggcacg agatcgcttc gcggatgtcc
     2821 ttggacctgg cactcacgct tccacgtttg gtggaaatcc tgtggtgatg gcagcagcac
     2881 tggccgtttt ggaactgctc atcgaaggtg gacttctgga gcgatgcgcc caactgggtg
     2941 cctacttcaa ggacaggctg tcgcagcttg cagagcatag ccccaaagtc aaagaggtgc
     3001 gtggcgcagg tctcatgctg ggcgtggtgc tcgacagtcc cgcaaagccc gtcatgatgg
     3061 cgcttcggga aaagggcatc ctgtgtgcga cggcgctgga tacggtggtg cgttttgtcc
     3121 caccattcgt gattgagaag gcggacattg accgaacggt tgatgcgctc ggatccattc
     3181 ttaagagcct ttgatgaaaa tcgggattga catcagcgag accaccggcg agaagacggg
     3241 cgtcggctac tatacgaccc atctggtgga agccctcgcg gctgttgacc gtgtaaacca
     3301 gtacacgctc tatccagtct tttttgacaa tttcccgccg acctatgata gggcaacttg
     3361 cccggaacaa gacaatttca gtctgtttgg caagagtctg tcccgcagaa agctgaccca
     3421 tctatggcac aagtccggga tgccaaagga gcaactcttc ggacaacagg atgtctttca
     3481 cagtacaacg ttctcatctc ctgaacttac cgaatcgtcc ttgatcgtca ctctttacga
     3541 tctctccttc tggacacacc cacagtgcca ccgaccaatc aatcggcgtt attgtgccga
     3601 gcgttgttgc cttgccgctc ggcgcgccag tcgcttgatc gccatctccg aagcgaccaa
     3661 acgggatatc ctcaagtacc ttcatgtgcc cgaagaacgt gtcgtggtaa ctccgctggc
     3721 agcgcatcca cgtttcaggc cggtcgaaga cgcctggtgc aaacatcagg tgttgatgaa
     3781 atacggtttg ctcaccgact acatcttctc tctcggttcc ctggagcctc gcaaaaacat
     3841 cgcgacgctg attcgagcct actcggcgtt gccggatcgt ctgcgccgaa caacacatct
     3901 ggcgattgct ggtgccaaag gttggaagaa ctccaacctg ttcgccttga tcgagcagct
     3961 gagcctgggc gactgcgtca aagtactggg atatgttccc gatgatgatc tgcctgcact
     4021 gtatagttcc gcttccgttt tcgtctatcc ctctttgtac gagggattcg gattgcccgc
     4081 gctcgaagcg atggcttgtg gtgcaccggt gattgtttca aacacgtctt cgctgcctga
     4141 agtggtagga acagcgggga ttcagattga accaaccgat gtcgaagccc tgacacaagc
     4201 gatcctgcgc gtgctcttgc agcctgatct ttcagaggaa ctccgccgaa aaggtatcga
     4261 acaggcacac cgattctcct ggcagcgaac cgcagagctg acactccaag cctatgttga
     4321 cgctgccgcc gagaaaaatc ggcgaaagtg accgattccc aggtacggtc agagcgattg
     4381 catccccatg tggcattctg aaggcacttc gggcagattg ttgcacctcg tgatatcact
     4441 tgcaggactt gcggcaggaa tgcattgttc tataattgaa ccggcgctgt attaagactg
     4501 aatgggcgtg ccagcctgca agaacgggac tgaaagtcta tgcctttcca gcaactgaga
     4561 acaatgcatt atccgatctc actggaggag atccacgccc gtgtggcacg caaaaacgac
     4621 aggcgtgccc tcaagaagac caatggcgca aagcatccct ccctctttga ggaacatcct
     4681 gccgaaaacg ttcatgaaac agtcaatgag tttggagaaa cgcttgttcg tgttgatgga
     4741 gatattccgg ttgatctgcc cattgccgaa ggcgaccggt ttctgttcat cagttacgat
     4801 cagagcattc tgacgcacgg attgcataag tatcccgcca aattcttccc tgaacttccc
     4861 cgatggttga tccggcgttt ctcgaaaaag gggcagttgg tcttagatcc cttcgccgga
     4921 agtggaacaa ctaatctcga atcgcttctt ctgcacaggc attcggtcgg aattgatgta
     4981 gacccgttct ctcggtttct ggcacgggtg aaaacaacag cgctcgacaa agaagacgtg
     5041 ttggcagcgc aggatgaagt tcttcgccga tgcgtgcaat ttgctccgga aaaggttcgg
     5101 ccgactgacg tgccgaactt cccctatcgg gacagctggt ttgacccgga gatcactctc
     5161 gagttgactt acctgaggaa gacgatagac gacttgaact gctctcagga aacggcggat
     5221 tttctcagaa tctgcctgtc ctcgatagtt cgctctgtct ccaatgctga tgacaactgc
     5281 accagaaccg tcatccgcaa gaggctcaac aagattgtcc tgccttctga tgcgctgagg
     5341 cgatttgcag aaacggttat gctcaacgtg cccagggtca tcgccttttc ggagcgccga
     5401 cccgagaatg ttcgggtcga attcccgcca gacatggatt ccagaagcat gcgttttcct
     5461 gatggcacct ttgatcttgc ggtgacctct cccccctatg ccaacgctgt agactatccg
     5521 agaacgcatc aactggaaat ctactggctc gggctggcgg aaggttcgct caccccactg
     5581 aagagaaaac acgtgggcac agagagcgtg tcagccgccg attataccta tctccatcag
     5641 acgggacatt cgcgggcgga tgtcgtactg tcccaaattt tcgagaaaga tccccgccga
     5701 tcctacattg ctttcaagta tcttgacgat atgcgccaga atctgcgaga ggtgtttcgc
     5761 gtgctgaagc gaggggcagc ctacattgtc gttgtcggta acaatcggat ccggggcgag
     5821 ctttttgaga actggaagta catcatggat ctcgctgagg aagttggctt tcacgtcgaa
     5881 aactacttcg gttcggagat catcaagcac ttcatcaaag ttccgcggga agaaagaatc
     5941 aacacggact ggatcctcat attgcgaaaa ccggaggtcg ggtgttcggc gcccagcggg
     6001 tcggcactcc cagaaaggaa aaagaaatga gactgggtgg tcctgttttt gacaagtatg
     6061 attcaccggg ggagtgggtc agagccgtgc gctcgctggg ctatcgcgct gcatactgtc
     6121 cgcttggcct cgatgcgcca caggatgtga tctgtgccta tgaacacgct gcggagcaag
     6181 ctgacatcgt gattgccgaa gtcggcgcgt ggagcaatcc attgagtccg gacgatgatg
     6241 ttcgcaagca ggctgttgag aagtgcaaga gaagtcttgc tctggcggat gccataggcg
     6301 ctcgctgttg cgtgaacatc gccggttcgc gcggtgcgaa gtgggatggt ccatgcaaag
     6361 aggatttgac ggatgagact tttgacgcca tcgtcgaagt agtgcgtgat attcttgatg
     6421 atgtccaacc acagagagcc ttctacacac ttgaaaccat gccgtgtatg tatcccgatt
     6481 ccaccgacgc ctacatagat ctgattcgag caattgaccg aaaacaattc gcggtgcatt
     6541 ttgatccggt caatctggtc tgcagcccac ggcgctattt caacaacgga gatctgatcc
     6601 gtgacttcgt cgcgcgtctg ggatcgcaca tccgatcctg tcacgccaaa gacatattgt
     6661 tgcaggatcg gttgaccgtt catctggacg aagttcgtcc cggacagggg gcactcgact
     6721 atagcgcgtt gctaagggag gtctcccatc tggacaaaga tgtgcctgtg atgctcgaac
     6781 acttgtcaag tgccgacgag tacaaggcgg ctgcggaata tatacgatcg gtcgctgcag
     6841 cgacgggggg ttctctatga atcaccacgc catagtcatc acagcaaagg agcaggcaga
     6901 tctgttgccc atcgaggaac ccgctgcgct gaaaactcat gaggtgcggg ggaataccct
     6961 ggtcactctg ataagccctg ggacggagat cgcgtggagt tatcttggcg tccagcagga
     7021 tcatgcccat gcatttccaa agcgcctggg ttacgctgct gtgttgcgcg cggaacaagt
     7081 tggttccgag gttcatgatc tggaaccggg aacttctctt ctctgcatgg gtaaccatca
     7141 gagctatcaa cagacagagg cactcgcagc tgttcccatc ccaccggaat tacccgctca
     7201 tgaagccgta ttggcacgac ttatgggagt cacaatgacc acgctgatga cgacctcggc
     7261 acgaccgggt gacctcgtcg tcgttactgg tgctggtccg gttggctatc tgggtgctca
     7321 tctttttgcg ctctccggct atgatgtctg cgtcgtcgaa cccaatgaga gacgccgagc
     7381 tggcgtgcaa cgctcgggta tcacaaccgt ccatccgacg atgccccttg aagacaaaga
     7441 gataaagggg caggtcgccc tggttctcga gtgttcgggg cacgagcagg ccgcactgga
     7501 tggtgcacgg atcg
//
LOCUS       DAOQUC010000015         7472 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_10888_length_7472_cov_3.539706, whole genome shotgun sequence.
ACCESSION   DAOQUC010000015 DAOQUC010000000
VERSION     DAOQUC010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7472
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10888_length_7472_cov_3.539706"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..705)
                     /locus_tag="PKH07_00510"
     CDS             complement(<1..705)
                     /locus_tag="PKH07_00510"
                     /inference="COORDINATES: protein motif:HMM:NF017390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HNT33464.1"
                     /translation="MIPSSALFSTPLIRALGWALLHSLWQGAAVVAVLFPLLWALRKQ
                     TPNTRYVVLCIALILILVLAAGAFYECLPGISASRLIVESTHVSSVSSVPQTGVKAAL
                     AVSESRLQQTGEGERVALAFSKDLARPQQFTAPYLLELALPWLGLFWLTGVAVLSLRL
                     VWGWRQMRSLTRRSVRPVAKTMDDILRRLCRQIRVSQTVHLIESALVEVPTVVGWLRP
                     VILLPASVFTGLTQQQL"
     gene            complement(702..1091)
                     /locus_tag="PKH07_00515"
     CDS             complement(702..1091)
                     /locus_tag="PKH07_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015345810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HNT33465.1"
                     /translation="MRYPQPPRPTDAELNILRVLWERGPSTVREVQEQLNRSKLTGYT
                     TVLKLLQIMTEKELVSRDESERSHVYEAKYPETLTQNQLVCDLLERAFGGSMSKLVLQ
                     ALDARKASAEDLEEIRRILDQLQGETK"
     gene            1344..2795
                     /locus_tag="PKH07_00520"
     CDS             1344..2795
                     /locus_tag="PKH07_00520"
                     /inference="COORDINATES: protein motif:HMM:NF012622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I domain-containing protein"
                     /protein_id="HNT33466.1"
                     /translation="MPKRILCCLVAVVVALISLCPYRAWALEELAAPNLLSPFNGMQF
                     VVSDFQEGNIVLKWSSVPGAVSYFLQVKGPNLYNPQDELDGNGQFDKTVFGTQFTLTN
                     STLAGEYTWWVQAKDASGALGSPSVPWRMLVFQSSLPTDTPTVPYPRYDINGDKVVDA
                     RDLACFALGEWTDQNPDLRCDVDWDGKVDADDLLLLLRNWSVRANLVPTPTPTSFLSA
                     PVLLFPEDNALLPIGSPPGLIFRWYSVAGAGGYRFQLRFDSPNMASFTEVFETSKTEV
                     LWKHYSTSGAGFGWWKVCAIEPDGDRGPWSEEWGFEIPTPTPTPSPTPLPDSSMPADI
                     NRDQTVNSTDLFSLALVWKQASSSTKFNYSADLTGDGVIDARDILLVLKDFRLNTPGP
                     GAVTLVYPGQNQSVRYSNLQSPGLQWQAIQGVTRYHVHVDDPTGRYPRDLFISGTSTP
                     LGVTTLGQYRWRVRAFEPEIGTWSAVRAFRFVQ"
     gene            2851..4194
                     /locus_tag="PKH07_00525"
     CDS             2851..4194
                     /locus_tag="PKH07_00525"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HNT33467.1"
                     /translation="MRHGWLYVSEHDLKIEQAQLEQEGKAIPAALVKEFRTLSKVDLH
                     QPKNQQRATELLEASVRLPLRKDYPYREPSDLESIRKERPKRVSLPAKRMTRSRMEDH
                     FLGAWLGRCAGCLLGKPVEGMKSDAIRGYLTALNQFPLSDYFSAKAPKKTLEKYGVNP
                     RRPFIENVSCMVEDDDTNYTVTGLVIMKRHGRDFQPHHVADFWMNNLPILHTCTAERI
                     AYRNFVIGIDPPASASYVNPYREWIGAQIRADFFGYAALGNPRLAAEFAWRDACISHI
                     KNGIYGEMWVAAMLAAAPFCETPDEVVAQGLMQVPKASRLAERVAAVRQWYRQGVSYD
                     DAVAKIHQEWNEYFGHDWCHTISNAMLVTVGLLWGELDYERSICRAVQPGFDTDCNGA
                     TVGSIVGMMLGAKKLPTKWISPLNDRLQTGVHGYFEVSLSELAKETCVIHKAVTA"
     gene            4581..4793
                     /locus_tag="PKH07_00530"
     CDS             4581..4793
                     /locus_tag="PKH07_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016477161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNT33468.1"
                     /translation="MSIEITLDLMLARRKMKLTDLSERIGISVTNLSLLKTGKVKGIR
                     FSTLDAICRELNCQPGEILQHVPDFV"
     gene            4853..5419
                     /locus_tag="PKH07_00535"
     CDS             4853..5419
                     /locus_tag="PKH07_00535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33469.1"
                     /translation="MAGSCGSDYKVAGFLSPVGAVLGLSGFFVCFSPAPDIFSIPFFF
                     FAAIIEIYVYLRFKDFLNRHYDFSRVNKLIAFLIIFCVLDFTLGVLDSLIPDSKGELA
                     LTGTLAFAILIPGGIAQIMFCRRLLRLNGTLHGLLKPFAYTGMVIAIALLSVVFVLVG
                     IFLTPVYSTLLGIILIKGPEQAEIPEFV"
     gene            complement(5545..7128)
                     /locus_tag="PKH07_00540"
     CDS             complement(5545..7128)
                     /locus_tag="PKH07_00540"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HNT33470.1"
                     /translation="MKLILITATTMLLMALTMGRTALADLNERITYIKDHFGEMHFKP
                     EGGFILRKGQVLPNLVWDEPEVAAELLENTVIPTRWFDSDLNEVTTAEKTGRYYAYGE
                     APAPEGYPLCRAMTCCCLQQDEDLHMLAVYRYPWDERSTKEVVKGWIESEEEVITLCG
                     KLDSKPPYQPERPGQWLMENASVHVRLKRKIRNITAPPVEVKARRLEGEPAPVLFTGK
                     VELTESDSEFKAKMEDVFTRWHQSSGEPMSVVVARKGNVIVSKAYGEMNGKPVTVNTP
                     MFIHSAMKPIMGLQLAMYVDREIIDLDEPIGNYLPEFDTPRDKNLTFRVAQVHTTGIH
                     FPWALAFSRHFYFNPWHEALIAKCPRDWNPGDRYQYGCVGMSLSVRAIETVTGKNYWE
                     AMEEQLFEPLGIKDMYPGGSGFSAEDFARLGVLVANKGKYGSWGLFSEKTYESIMPVQ
                     LQEYIPNLDQRIIRGIGFTTMVPGFYGHSGGCGTRLLVDPENHLVYAMTRMEGDDKFT
                     EFNKEATDLLQELRKEYIR"
BASE COUNT         1757 a         1991 c         1895 g         1829 t
ORIGIN      
        1 cagttgctgc tgtgtcagcc cagtgaagac gctggccggc agcaggatga cgggtctcaa
       61 ccaaccgaca accgtcggaa cttcgacgag cgctgattcg atcagatgaa cggtctgaga
      121 aacgcgaatc tgacgacaca gacgccggag gatatcgtcc atcgttttgg ccacgggacg
      181 aacactccga cgcgtcaggg agcgcatttg tctccatccc caaaccagac gcagcgagag
      241 aaccgccaca cctgtgagcc agaaaagacc gagccacggt agtgccaact caagcaggta
      301 gggtgcggtg aattgctgtg gtctcgccag atctttggaa aaagcaagcg caacacgttc
      361 tccttcgccc gtctgctgca acctgctttc agagactgcg agcgctgcct tcacccccgt
      421 ttgcggtact gaggacacag atgagacatg cgtggactcg acgatgagtc gcgatgccga
      481 gatcccggga aggcactcgt agaaagcacc agcagcaagc accaaaataa ggatcaatgc
      541 gatgcacaga accacgtacc tcgtgtttgg cgtttgcttt ctcagcgccc aaagcagtgg
      601 aaacaagacc gccacaacgg ctgcgccttg ccacaaagag tgcagcaacg cccatccaag
      661 ggctcggatc agaggggttg aaaacagtgc gctggatgga atcatttcgt ttctccttgg
      721 agttgatcca ggatgcgtcg gatctcctcc aggtcttctg cggaagcctt cctggcatcc
      781 aaagcctgaa ggactaactt ggacatcgag ccgccaaaag cccgctccaa aaggtcacag
      841 accaattggt tctgggttag ggtttcagga tacttggcct cgtagacgtg cgaacgttcc
      901 gattcgtccc gactcacgag ttctttctca gtcatgatct ggagcagttt cagaacagtg
      961 gtgtaaccgg tcaacttcga ccgattgagc tgttcctgca cttctcgaac cgtactcggc
     1021 cccctttccc aaagaacccg caggatgttc agttcggcat ccgttggacg gggcggttgt
     1081 ggatatctca tcttgaaaaa tgcctccatt aagtaataga cgaagaaata attacgaagt
     1141 tcttcgtatt tgtcaacgaa aatcttcgta gaaccaagaa ttccggccac agcgggtcgg
     1201 tcgccgaaga cgcacccgat gcgatgccct ttcattttct tcgtgacact tttgttcaat
     1261 ctgtcataga atcctcaaaa caaggtcttg cgccttggat tgtcggtgac agagcatcta
     1321 ctgagcactc ttggagacgt aaaatgccaa agagaatact ctgctgcctg gtggcagtcg
     1381 ttgtggctct gataagtctt tgtccgtaca gggcttgggc tttagaggag cttgcagcgc
     1441 cgaatctgct cagcccattc aatggaatgc agttcgtcgt ttcggatttc caggaaggca
     1501 acatcgtgct caagtggtct tccgtgcctg gggcggtttc gtatttcctt caggtgaaag
     1561 gtcccaatct ctacaatcct caggatgaac tggacggaaa cggtcagttt gacaagactg
     1621 tttttggaac ccagttcact ctgaccaaca gcacccttgc aggggaatac acctggtggg
     1681 tgcaagccaa agacgcgagc ggggcgcttg gttcacccag cgtgccgtgg cgtatgctcg
     1741 tttttcagtc atcacttccc acagatacac caacggttcc ctatccaaga tacgatatca
     1801 atggggacaa ggttgtagat gcccgtgacc tggcttgttt tgcgttggga gaatggaccg
     1861 accagaatcc cgatctgcgt tgcgatgtgg actgggacgg caaggtggat gccgatgatc
     1921 ttttgttgtt gcttcgcaac tggagcgtaa gagcaaacct tgtgcccaca ccaacaccaa
     1981 cctcttttct gagcgcgcca gtgcttctct ttcccgaaga caacgctctg ctccccatag
     2041 gaagtcctcc tggactgata ttcagatggt acagcgttgc cggagcaggt ggctaccgat
     2101 ttcagctacg atttgattcg cccaacatgg cttcattcac cgaggtattc gagacctcga
     2161 aaacagaagt tctctggaag cactacagca cctctggcgc tggctttggt tggtggaagg
     2221 tctgtgccat tgaacccgac ggcgaccgtg gcccctggag cgaggaatgg ggctttgaaa
     2281 tcccaacgcc gacgccgacc ccgtctccaa ctccactgcc agattcctcg atgcccgcgg
     2341 atatcaaccg cgaccagaca gtgaactcaa cggacttgtt cagtctcgcg cttgtctgga
     2401 agcaagcttc ttcttcaaca aaattcaact atagcgctga cctgactgga gacggagtca
     2461 ttgatgccag ggatatcctt ctcgtgttga aggatttccg tctcaacact cctggacccg
     2521 gagcggtcac gctcgtctac cccggccaga accaatctgt ccgatacagc aaccttcaga
     2581 gtccgggact tcagtggcag gccatacaag gagtgacccg gtaccacgtc catgtggatg
     2641 atccgacagg cagatacccc agagatctgt tcatcagcgg tacttcaacg cctctgggtg
     2701 tgacaacatt gggacagtac cgttggcgcg tccgggcatt cgagccggag atcggaacct
     2761 ggagcgcagt cagggcattt cgatttgttc agtgaaaaca gcggataggc ttgacctatc
     2821 cgacaaccgc ttgatgtgga gagacagagt atgagacacg gatggcttta tgtttctgag
     2881 cacgatctga agattgaaca ggcgcagttg gagcaggagg gcaaggcaat tcctgctgcc
     2941 ctggtcaaag agttccgcac actttcgaag gtggatctgc atcaacccaa aaaccagcaa
     3001 cgggcaactg aactgctgga agcgtcagtc agactgcctc tgcgaaagga ctatccctac
     3061 cgcgaacctt ccgatcttga gtcaatccga aaggaacgac cgaaaagagt ctctctgcct
     3121 gccaaacgca tgactcgatc caggatggaa gatcatttcc ttggcgcttg gctgggacgt
     3181 tgtgccggtt gcctcctggg aaagccggtt gaggggatga aatcggacgc catacgcggc
     3241 tacctcactg cgctcaacca attccccctt tcagactatt tcagcgcaaa ggctccgaaa
     3301 aagactcttg agaaatatgg tgtcaacccg cgcagaccgt tcattgagaa cgtcagctgt
     3361 atggtggaag acgacgacac caattacacc gtcacaggac tggtgatcat gaagagacat
     3421 ggtcgagact ttcaacctca tcacgtcgca gatttctgga tgaataatct cccgatactg
     3481 cacacctgca ccgcagaacg catcgcctat cgaaacttcg tcatcggcat tgatccgccc
     3541 gcctctgctt cctatgtgaa tccctatcgg gaatggatcg gcgctcagat tcgggcagac
     3601 ttcttcggct acgccgcact gggaaaccca cgacttgcgg cggagttcgc ttggcgcgat
     3661 gcctgtatca gccacatcaa gaacggaatc tatggcgaaa tgtgggtggc agccatgttg
     3721 gcagcagcgc ccttctgcga aactcctgat gaggtggttg ctcaaggtct gatgcaggtt
     3781 cccaaagcca gcaggctcgc cgagagagtc gcggcagttc ggcaatggta tcggcagggt
     3841 gtctcatacg acgacgccgt ggcaaagatc catcaggaat ggaacgagta cttcggtcac
     3901 gactggtgtc atacaatttc caacgccatg cttgtcaccg tcggactgct gtggggcgaa
     3961 ctggattatg agcgatcgat ctgccgcgcc gttcaacctg gatttgacac cgactgcaac
     4021 ggagcaacgg taggttctat tgtcgggatg atgctgggcg cgaaaaagct ccccacaaag
     4081 tggatttccc cgctcaacga ccggcttcag acgggcgtgc atggatactt cgaagtctct
     4141 ctgtccgagt tggcgaaaga gacttgcgtc atccacaagg cagtgacggc ttgaggcagt
     4201 cggtgcgggg gcgtacagtc ctagattcgg gttagtaggc tgtcatactc ggcattggaa
     4261 cctatccaaa accagtgagt agctggtgga gcgagtccgc gagttccacc ctctttcttc
     4321 tcaagaacat gttggaacgc gctcggtcgg cagtcagcat cctgggttcc cccgaaccca
     4381 ggattgatcc ccactcgaat gcgcactgag ttatatggtt ggcgttcgag aaaaagcgaa
     4441 gacagcccgt tggtcgcgac ccacactgca atctgaagca atctaaatcc tggtacaggc
     4501 tatctgtaaa agataaataa ctttatgtaa aacataaaaa tatacttgac aaacataaag
     4561 aatggattat tctataccgc atgagtattg aaatcactct ggatctcatg ttagcgagac
     4621 ggaagatgaa gttgacggac ttgtcagagc gtattggcat ctccgtgacc aacctgtctc
     4681 tcctcaagac gggaaaggta aaggggattc ggttcagcac ccttgacgcg atttgtcgag
     4741 agttgaactg tcagccggga gaaatccttc agcacgttcc cgactttgtg tgactttcgc
     4801 tcgctttcac agagaccgga gaccaaagtt agtattcttg gaggaaagtg tcatggctgg
     4861 ttcgtgcggt tcagactata aagtagctgg ctttctatct ccagtgggtg ctgttctggg
     4921 tctatctggg ttttttgttt gtttctctcc tgctccagac atcttttcga ttccattctt
     4981 tttcttcgca gccatcattg agatttacgt ctatctccgc tttaaggatt tcctcaaccg
     5041 acactatgac ttctctcggg tgaacaagct catcgctttc ctgatcatct tctgtgtcct
     5101 ggatttcacg ttaggtgttt tggattccct gatacccgac agcaagggag aactggctct
     5161 gacaggtact ctggcatttg ccattcttat tccgggaggg attgcgcaga ttatgttttg
     5221 ccgacgactt ctgcgactca atggaacact tcacggcttg ctcaaaccct ttgcgtacac
     5281 tggaatggtc atcgcaattg ccctcctgtc tgtagtcttt gtcctcgtag gcatcttcct
     5341 gactccggtt tactcgacac ttctcggaat cattctgatc aaaggtcctg aacaggcgga
     5401 gatacctgaa tttgtgtagc cctgtaaacc gcgctgagcc cgacattccg gttgggaacg
     5461 cgtcacaatt gttttggcgg gtacaacacg cctcacggct gcttcgatgg catcgcagac
     5521 gcaaagggac gttttggtct tattctatcg gatatactcc tttctcaact cctgcaacaa
     5581 gtccgtggct tctttgttga actctgtgaa tttgtcatcg ccttccatcc gggtcatggc
     5641 gtagaccaga tgattctcag gatccaccag caggcgtgtt ccacatcctc cgctgtgacc
     5701 ataaaatcca ggaaccattg ttgtaaaccc gatgcctcgg attattctct ggtcaagatt
     5761 cggaatgtat tcttgaagtt gaaccggcat tatggactca taagtcttct ccgaaaaaag
     5821 cccccatgag ccatatttcc ccttgttcgc caccagaacg ccgagccgcg caaagtcttc
     5881 ggctgaaaag ccgcttccgc ccggatacat atccttgata ccaagaggtt caaaaagttg
     5941 ttcttccatt gcttcccaat agttcttccc agtgaccgtt tcaatcgccc ttactgaaag
     6001 actcattcca acacatccat attggtacct gtcgccggga ttccaatccc tcggacactt
     6061 ggcgatcagg gcttcatgcc aagggttgaa ataaaagtgt ctcgaaaagg caagcgccca
     6121 cggaaagtgg atgccggttg tgtgaacctg tgccacgcga aacgtgagat tcttgtccct
     6181 cggagtatcg aattcgggaa gataatttcc tatcggttca tccagatcga tgatctcccg
     6241 gtctacatac atcgccagtt gaagtcccat gatcggcttc atcgctgaat gaatgaacat
     6301 aggagtattg accgtaaccg gctttccgtt catctctccg taagccttcg aaacgatcac
     6361 gttacccttt cgggctacga cgacactcat cggctcgccg gaactttgat gccatcttgt
     6421 gaagacatct tccatcttcg ccttgaattc gctgtccgat tcagtcagtt caacctttcc
     6481 ggtgaaaaga accggcgccg gctcgccctc gagtcttctt gccttcactt caaccggagg
     6541 ggcagtaata tttcggatct tacgtttcag acggacatgg actgaggcat tttccataag
     6601 ccactgaccg ggacgttcag gttgataagg aggcttggag tccagttttc cacagagcgt
     6661 gatgacttcc tcttcacttt cgatccatcc tttcacaacc tctttggtac ttcgctcgtc
     6721 ccatggatac ctatagacag ccagcatatg aagatcttca tcctgttgca gacaacaaca
     6781 ggtcatggct cgacacagag gatatccttc gggggcgggc gcctcaccat aggcgtagta
     6841 cctgccggtc ttctcggctg ttgtcacctc gttcaagtcg ctgtcaaacc atcttgtcgg
     6901 gatcaccgtg ttttcaagaa gttcggctgc gacttccggt tcatcccaga caagattcgg
     6961 gagaacctga ccctttctta gaatgaaccc tccttctggc ttgaaatgca tctcgccaaa
     7021 atgatctttg atataagtga ttcgttcatt caaatcagcc aatgccgtcc tccccattgt
     7081 caaagccatt agaagcatag ttgtggctgt gatcaaaatc agtttcattt tccatccttt
     7141 gtgattcagt ggtgagggtg aaacaagttc gaccctgcga tctgtcagaa tactagcaga
     7201 acgactcagc agttaaagag gaaccacgcg ggccacatgc tccaagcccg tagcctgcct
     7261 gggttgcccc gaacccagga tggttcttta cctttgtctg cgacggggtg attttcttga
     7321 ctctgctttc gaaagaagcg cataatcaaa atggtgctgt gggcatctgt ggaaggtaag
     7381 atttggagtc gcgctttttg gagcgggcaa ggaagaaaag caacttcgat tatcgagggc
     7441 acgtatgcta ggattggtga aaaccatcaa tt
//
LOCUS       DAOQUC010000016         7369 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_11091_length_7369_cov_3.453240, whole genome shotgun sequence.
ACCESSION   DAOQUC010000016 DAOQUC010000000
VERSION     DAOQUC010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7369
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11091_length_7369_cov_3.453240"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            321..1616
                     /locus_tag="PKH07_00545"
     CDS             321..1616
                     /locus_tag="PKH07_00545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33471.1"
                     /translation="MREFQVIHNIASRLASKAAILLCLMSLCIASADAVTEATMGIEK
                     LKEGSSVGTVEVRGVMAGSFNPELWNQARFNLVPDTRNPLLAPLPGKNRNIYAPSAVE
                     VGNGWRLFYCAWDGVATGNDRIYSVMTSDFLSFSERHTVIENGDFVHVCNVNAIRLPD
                     NSLSMICTAYPDKDGLNKPAFFSSPDGNIWNKSSSPYSATHSDIVSLDGYEVYPQADI
                     NGMNVILFEEGQYRLYFANFRDFGKIYRASSTDGKQYTFEGKVLDKSAAVNDVKKFRV
                     GEETWYLMGLHMNCNLMWYTLSQNPSDFPEPKQLASSFNAGERYIVALGWVVQGEQEE
                     QGRRLLGVLYGAGYHHGLAGNRIFARWLQKRVVFRAADGREFEGASALGPDRQILSLS
                     KGEEIAGTLQVYSEDGHTLLVEFPEQVLTEGAVYQLTIP"
     gene            1771..2508
                     /locus_tag="PKH07_00550"
     CDS             1771..2508
                     /locus_tag="PKH07_00550"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNT33472.1"
                     /translation="MDDKNVSPQQDRHGQKLEALLEAARAVLEYSRFADSARAIFDQA
                     KKITGATAGYVALLSETGEENEVLFLDAGGQDCTVDPSLPMPIRGLRSESYAKKATVY
                     DNDFLNSEWLQYLPEGHVPMQNVLFAPLIIEGKAVGIMGLANKPSDFTEDDARMATAF
                     GDFAAVALNNSWNLEKLQDTVNHLQEALAEVKTLKGIIPICCVCKKIRDDRGYWSQLE
                     AYIHKHSDADFSHGLCPECEEKLRNEI"
     gene            2640..2725
                     /locus_tag="PKH07_00555"
     tRNA            2640..2725
                     /locus_tag="PKH07_00555"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2675..2677,aa:Leu,seq:gag)
     gene            complement(2800..2897)
                     /locus_tag="PKH07_00560"
     tRNA            complement(2800..2897)
                     /locus_tag="PKH07_00560"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2861..2863),aa:Sec,seq:tca)
     gene            complement(2974..4212)
                     /gene="hydF"
                     /locus_tag="PKH07_00565"
     CDS             complement(2974..4212)
                     /gene="hydF"
                     /locus_tag="PKH07_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HNT33473.1"
                     /translation="MVTAPKGFRLHIGFFGRRNVGKSSLLNALTRQQVSIVSDVAGTT
                     TDPVEKPMELLPLGPVLFIDTAGIDDVGALGELRVQKTKQVLDRTDLGVIVAASGDWS
                     DFEEAILAALQERSIATVVVFNKCDLHPPSLATLDRLNARKIPCVQTDCLQGRGILDL
                     RQVLLDNAPDDFINAPAIVSDLVGPGEMAVLVVPIDKEAPKGRLILPQVQSIRDLLDH
                     DSMCLVVKERELRAAIQSLNKPPRLVVTDSQAFLKVSADTPREIPMTSFSILFSRFKG
                     DLVTQVLGTLAVDSLKSGDRILVSEACSHHPIGEDIGRVKIPRWLTQYVGGKLEFVHV
                     QGHDFPEDLSPYKLIIHCGACMWNRREMLSRLLRCRRAGVPITNYGLVIAYSLGIFER
                     ALEPFPAALETYRETAKLGR"
     gene            complement(4232..5635)
                     /locus_tag="PKH07_00570"
     CDS             complement(4232..5635)
                     /locus_tag="PKH07_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015720163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HNT33474.1"
                     /translation="MSDAVRVEKDLLGQIHVPAEALYGIHTVRALGNFPLSGRLVHPE
                     LICAYGAVKLACANTNFALGIWDDDPEKAQAIIQAATEMMNGLLTEHILVDSLQGGAG
                     TSTNMNVNEVLANRALMILGEPFGNYQRVSPLDDINRHQSTNDTYPTALRLAMIRLLD
                     RLEQRLVSLQEAFQDKEKAFAHIVKVGRTEMQDAVLTTLGREMGAYAEAFNRDRWRIY
                     KCQERLRVINLGGTAIGTGLAAPRQFIFRVVDALREVTGIGLARAENLVEATQNADVF
                     VEISGILKACASSLLKIATDLRLLASGPEAGFGEIHLPEQQAGSSIMPGKVNPVIPEA
                     VSQAAMLVMGYDATLTMACSMGNLELNPFLPLVAVCLLNTCDVLANACDTFRALCVEG
                     IQADETRCRAHVESSIALVTALVAELGYERACAIARKAKQSGKSVKEIVLSENLLTEQ
                     QFNELTSPEAVCRLGNP"
     gene            complement(5730..6878)
                     /locus_tag="PKH07_00575"
     CDS             complement(5730..6878)
                     /locus_tag="PKH07_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020464449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HNT33475.1"
                     /translation="MLENIAILRDGRSKRVSSFDRTGRNKDCLNIGKGEEVVLADIKG
                     SGVIRHIWFTIMAGDNYLRRMVLRAYWDGMEHPSVEAPIGDFFGIGHAQLAPLNTAVF
                     SISSSDFFGPKAGMNCYWPMPFSDGARITVANECDGDVMAFYYYVDYDELDSPPQNMG
                     RFHAWWNRNNPCTPPKQAPKDPDVNLSDKDNYLIADIKGRGHYVGCNLSIHNLYGGWW
                     GEGDDMFMVDGVKWPPDMHGTGSEDYFGHAWGMQVQNAFIYNGVSYHKKGTEPAQINE
                     RITVYRFHINDPVLFHKSLRVSIEHGHANDRCDDYCSTAYWYQVLPHKKFPEFPKVED
                     RLPRPDVNVHVQAVDLPIPIRDGRHRSGMPFAPLDLVPQKGTRSRRSK"
     gene            7131..>7369
                     /locus_tag="PKH07_00580"
     CDS             7131..>7369
                     /locus_tag="PKH07_00580"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNT33476.1"
                     /translation="MEHEDKREAILKAAEEMVQNRRFHEVTLDEVAQAARVSKGTIYN
                     YFEDKEDLFFQLATHGHDQLCDAVRDCAASQTPTS"
BASE COUNT         1743 a         1980 c         1947 g         1699 t
ORIGIN      
        1 acctgcggat cgacccgagc acagccgttg gtgccacagt cgagatagac tatgtgctca
       61 tcccatgact gcaggagcag attgattctg tcttgtctgc gagagcagat cctgttttgc
      121 gtgattgcag aggcaggtca aacttggcct tggtacattc attgaggtgg gaagggtcac
      181 gctacagatg ccgagcaact ggcagcgtct gtctgttact gcctcttcca gccgaatctt
      241 gttgaatcaa tgggctccat tgcctactat ccaaagcggg agactgaata ccagattttg
      301 cgggcggagg ttttaatgtc atgagagaat tccaagtcat acacaacatc gcttcgagac
      361 tcgcttcaaa ggcagcaatc ctgctctgtc tgatgtctct ctgcatcgcg agtgcagacg
      421 ctgttacgga ggctaccatg ggcattgaga aactgaagga aggttcatct gtcgggactg
      481 ttgaagtcag gggagtcatg gccggatcat tcaacccgga actctggaat caggctcggt
      541 tcaacttggt tccagacacc cgaaatccac ttctagcgcc gctgccagga aagaatcgga
      601 acatctatgc cccttcggct gtggaagttg gaaacggatg gcgactgttc tactgtgcct
      661 gggatggtgt tgcgacaggc aacgatcgca tttacagtgt catgacctcg gatttcctgt
      721 cgttttccga acggcatacg gtcatcgaaa acggtgattt cgtccatgtc tgcaacgtta
      781 atgcgatcag acttcccgat aactctctca gtatgatctg tacggcctac cccgacaagg
      841 atggcctgaa caagcccgcc tttttcagca gcccagacgg aaatatctgg aataaatctt
      901 cttcaccata ttccgcaacc catagcgata tcgtgagtct tgatgggtat gaagtctacc
      961 ctcaagcaga tatcaatggc atgaatgtca tcctctttga agaaggacaa tatcggctct
     1021 acttcgccaa ttttcgagac tttggcaaga tatatcgcgc ctcaagcacg gatggcaaac
     1081 aatacacttt cgagggaaag gttcttgaca aaagcgcagc ggtcaacgac gtgaagaaat
     1141 tccgcgttgg agaggagacc tggtatctga tgggactgca catgaactgc aaccttatgt
     1201 ggtacacgct ctctcagaat ccctcggact ttcccgaacc caaacagctg gcttcgagtt
     1261 tcaacgccgg agagcgatat attgtcgctc ttggttgggt ggttcaggga gaacaggagg
     1321 agcagggcag aaggcttcta ggtgtgctct atggtgccgg atatcatcat ggtcttgccg
     1381 gcaacaggat ttttgcgcgt tggctgcaga agcgcgttgt gtttcgggct gccgatggtc
     1441 gggagtttga aggcgcttct gctttggggc ctgaccgtca gattctttcc ctctccaaag
     1501 gagaggagat tgccggaaca ctccaggtct attctgaaga cgggcacaca cttctggttg
     1561 agttccccga acaggttttg accgagggag ccgtctacca gctgacgatt ccgtaatctt
     1621 gcccgatggg gcaagtgaag gctcctcatc caggcggatg ttgacccgtt tttgattccg
     1681 tggcatcata taatcgagtc gtgagactcg ctcagggtcg tgtctgaaca catcagttct
     1741 gctttgaact ggaaaggaga tcggatgggt atggacgaca aaaacgtttc acctcaacag
     1801 gatcgccatg gccagaaact ggaagcgctg ctcgaggcgg cgcgcgcagt gttggagtat
     1861 tcgagatttg cggactctgc ccgcgcgatc ttcgatcagg caaagaagat cacgggcgct
     1921 acagcaggat atgttgcatt gctttcggaa accggtgagg agaacgaggt tttgtttctg
     1981 gacgccgggg gacaagactg cacggttgat ccttccctgc cgatgcccat tcgaggactt
     2041 cgctcggaat cctacgcgaa aaaggccact gtctatgaca acgatttttt gaacagtgag
     2101 tggctccagt atcttcctga ggggcatgtt cccatgcaga acgtcctgtt tgctccactg
     2161 atcattgaag gcaaagcggt cggaatcatg ggtctggcaa acaagccgtc ggatttcacg
     2221 gaagacgatg cgcgcatggc gactgcgttc ggagatttcg ctgccgttgc gttgaacaac
     2281 agctggaacc tggagaagtt acaggacaca gtcaaccacc tgcaagaagc gctggcagaa
     2341 gtcaagacac ttaagggaat catccccatc tgttgtgtct gcaagaaaat ccgcgacgat
     2401 agaggttact ggtctcagtt ggaggcgtac atccacaagc actcagacgc cgatttctca
     2461 catggtcttt gcccggagtg tgaggaaaaa ctgaggaacg agatttagtc tcttaccaag
     2521 acaaatttcc gcgggctctt gacaattccg cgaagcgcta tacactacag aacacttggg
     2581 caggaaccca ttgttcgccc tgagttgggc ggcggttggt tcctgttctg gtgcaaagcg
     2641 cccggatggt ggaacttggt agacacgtac gtttgagggg cgtatggcta acgccgtggg
     2701 ggttcaaatc cccctccggg caccatcgca gaaacaaaac gaagggagct gtttccttcg
     2761 tttttcattt tggttcccag caggagactc tgcagatact ggcggaggcg aatgggagtc
     2821 gaacccacct gtcccacaca aggtaagaca caaacggctt tgaaggccgc gggagccacc
     2881 gggccccagt cgcctccgaa caaagattca aactcaactc agcaagacta tcgttcgatg
     2941 aacagcgtgt gtcaagttgg agactcaccg tcattacctg ccgagtttcg cggtctcccg
     3001 ataggtctcc aaggcagcag gaaaaggctc cagcgcgcgc tcgaaaatac ccaggctgta
     3061 agcgatcacc aaaccgtagt tggttatcgg aacaccggcg cgacgacagc gcaacagacg
     3121 actcagcatc tcgcgacgat tccacataca ggctccacag tgaatgatca atttgtacgg
     3181 agacagatct tcggggaaat cgtgcccctg cacatgcacg aattccagtt tgccgccaac
     3241 atactgagtg agccatcgtg ggatcttcac acgtccaata tcctctccta tcgggtgatg
     3301 tgaacacgct tcagagacta gaatacggtc acccgacttg agcgagtcca cggccaatgt
     3361 tcccagcacc tgagtgacca gatctccctt gaagcgcgag aagaggatcg aaaaactggt
     3421 catcggaatc tcgcgtggcg tatcggcact gactttcaga aatgcctgtg agtctgtcac
     3481 aaccagtctc ggtggcttgt tcagcgactg tatcgctgct cgcagttccc gctccttgac
     3541 gaccagacac atcgagtcgt ggtcgagcaa gtcccgaatg gactgcactt gtggcaggat
     3601 gagacgcccc ttgggtgctt ccttgtcaat agggacaacc aacacggcca tctcgcctgg
     3661 tccgaccagg tcagagacaa tcgctggagc attgatgaaa tcatcaggcg cattgtccag
     3721 gaggacttgg cgaagatcga gaatgcctct tccctgaaga caatccgtct gcacacaagg
     3781 aatctttcga gcgttaagcc tgtccaatgt tgccagtgat ggcggatgga gatcgcactt
     3841 gttgaacaca accacagtgg caatgctgcg ttcttgaagc gcggcaagaa tcgcttcttc
     3901 gaaatcactc cagtctccag aagccgctac aatgactcca agatcggttc tgtcaagaac
     3961 ctgttttgtc ttctgaacgc ggagttcccc cagcgctccc acatcatcaa ttcccgccgt
     4021 atcaatgaac aacaccggcc ccagtggaag gagttccatc ggcttttcaa ccggatcagt
     4081 cgtcgtaccg gcaacatctg aaacgatgga cacctgctgc cgtgtcagtg cattcagcag
     4141 actggacttg ccaacatttc gcctgccgaa aaacccaata tggagacgga agcctttggg
     4201 ggctgttacc aagtgtttct gctcctatcg tctacggatt tcccagccgg cacacggctt
     4261 ccggactggt cagttcattg aactgctgtt cggtcaacag attctccgag agcacaatct
     4321 ccttcacact cttgcctgac tgcttcgcct ttctggcgat ggcacatgcc ctctcatagc
     4381 caagctcagc aaccaacgcg gtcaccagag caatcgatga ctcaacatgg gcgcggcatc
     4441 gagtctcatc cgcctgaata ccttccacac acagtgcccg aaatgtgtcg catgcgttcg
     4501 cgagcacatc acaggtgttc agaaggcata ctgcaaccag aggcaagaag ggattgagtt
     4561 caaggtttcc cattgaacac gccatcgtca gcgttgcatc gtatcccatc acaagcatcg
     4621 ccgcctgact gaccgcttca ggaatgaccg gattcacctt tcctggcatg atggatgaac
     4681 ccgcttgttg ttcaggaaga tggatttcgc caaagcctgc ttcaggaccc gaagcaagga
     4741 gtcgaagatc cgttgcgatc ttcagcagag aagaggcgca ggccttcaag atgcccgaga
     4801 tttctacgaa gacatcagca ttttgtgtcg cctcgacaag gttctccgcg cgcgccaatc
     4861 caattccagt cacttctcgc agggcatcca caactcgaaa gatgaactgc cgtggcgctg
     4921 ccagacccgt gccaatggcg gtgccaccca aattgatgac tcgaagtcgt tcctggcact
     4981 tgtagatccg ccagcggtcg cgattgaaag cttctgcata ggcgcccatc tcccgcccga
     5041 gagtcgtcag gacggcatcc tgcatctctg tgcgtccaac tttgacgata tgagcaaacg
     5101 ccttttcctt atcctgaaag gcttcctgaa gggacaccag tcgttgttcc agtcggtcga
     5161 gaagtcgaat cattgccaac cgaagagcag ttggatacgt atcgtttgtg gactgatgcc
     5221 ggttgatgtc atccagcggg ctgactcgtt ggtagttgcc gaaaggttcg cccagaatca
     5281 tcagggctcg gttggcgagc acttcgttga cgttcatatt ggtgctggtg cctgcaccgc
     5341 cctgaagtga atctacaagg atgtgctcgg tcagcagacc gttcatcatc tccgtggctg
     5401 cctggatgat ggcttgtgcc ttctccggat catcatccca gataccgagt gcgaaattgg
     5461 tgttggcaca ggcaagtttg accgctccat aggcgcagat gagttcagga tgaacgagcc
     5521 gaccgctcag agggaagttc ccaagcgctc gcacggtatg aatgccgtag agcgcttcgg
     5581 caggcacgtg gatttgaccc agcaaatcct tttcaactcg aactgcgtca ctcatggcta
     5641 ctcaccattc agagatgaat cagtctgttt ccggctgttt ctcggaacgc caccaaggag
     5701 gcgctcgaaa atgcaccctc cgctgccaac tacttgcttc ttcgcgatcg agtccctttt
     5761 tgcggcacga ggtcaagtgg cgcaaagggc attcccgagc gatgccgtcc gtctcgaatc
     5821 ggaatgggaa gatcaacggc ctgcacgtga acgttcacgt cgggtctggg cagtctgtcc
     5881 tcaaccttgg gaaattcggg aaacttcttg tgaggcagca cctgatacca ataagccgtg
     5941 ctgcagtagt catcgcaacg gtcgttggcg tgaccgtgtt cgatgctgac acgcaacgac
     6001 ttgtgaaaca gaaccggatc gttgatgtga aagcggtaga cggtgatacg ctcgttgatc
     6061 tgagccggct cggttccctt cttgtgatag gaaacaccat tgtagatgaa tgcgttctga
     6121 acctgcattc cccaggcatg gccaaaatag tcttccgaac cggttccgtg catatcgggt
     6181 ggccatttca cgccgtccac cataaacata tcgtcgccct ctccccacca gccgccatag
     6241 agattatgaa tggagagatt gcagccgaca tagtggcctc ttcccttgat gtcagcgatc
     6301 agataattgt ccttgtcaga aagattgacg tcgggatcct tcggtgcctg ctttgggggc
     6361 gtacagggat tgttgcgatt ccaccaggca tgaaatcgtc ccatgttctg cggtgggctg
     6421 tccaactcgt cgtagtccac atagtaatag aatgccatca catcaccgtc gcattcgttg
     6481 gccacagtga tcctcgctcc atcggaaaag ggcatcggcc agtagcaatt catccctgcc
     6541 ttgggtccaa agaaatccga ggatgaaatg ctgaaaacgg cggtgttgag cggcgccagt
     6601 tgggcatgac cgattccgaa aaaatccccg atgggggctt cgacgcttgg atgctccata
     6661 ccgtcccaat aagcccggag caccatccgc cgaaggtagt tgtcacctgc catgatcgtg
     6721 aaccaaatgt ggcggatgac accagagccc ttgatatctg ccagaacaac ttcctcaccc
     6781 ttgccgatgt tcagacagtc cttgttcctc cccgttcggt caaaactcga aacgcgtttc
     6841 gaacgcccgt cacgcaaaat cgcaatgttc tccagcatct gttcggaatc cttcctgcag
     6901 gacagatgcg tgcagatggc acacagacct gcatccactc ataagaattg gaaaccagac
     6961 ttttctcagt ctggtatcac ttccacgcta tcggccgtgg ttcttgggta ggataataac
     7021 catggcttca gctgtcgtcc atcggtctct ctcagaagac tgatttttct tgacaccggc
     7081 caagccacct tgatatgaat actgaccagt cagtactgat agagggtaag atggaacacg
     7141 aagataagag ggaagccatc ctgaaggcag ctgaggagat ggttcagaat cggcgattcc
     7201 acgaagtcac cttggatgaa gtggcacagg cagctcgagt cagcaagggc accatctaca
     7261 actactttga ggacaaggaa gatctgttct ttcaactggc aacgcacggg catgatcagt
     7321 tgtgtgatgc cgttcgggac tgtgccgcct cacagacgcc cacttcttt
//
LOCUS       DAOQUC010000017         7306 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_11211_length_7306_cov_3.227555, whole genome shotgun sequence.
ACCESSION   DAOQUC010000017 DAOQUC010000000
VERSION     DAOQUC010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7306
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11211_length_7306_cov_3.227555"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..591
                     /locus_tag="PKH07_00585"
     CDS             <1..591
                     /locus_tag="PKH07_00585"
                     /inference="COORDINATES: protein motif:HMM:NF020133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HNT33477.1"
                     /translation="RLCDDALGREDLLTLATEIEGHPDNVSPALCGGLTISYMADREV
                     VVCPCRISMPLKVAVVLPAAKKESTSQTRKLFPRKVDLADALHNQSRTAAIVAGLLQG
                     DLKQGRTLFDDRLHQRQRCVLMPWLFHAIEAAYDAGALGAFLSGAGPAVAALCRTEDL
                     AVVLEAMDAAISAQGMPNRRFGLDIAAEGAEFLSVR"
     gene            613..2337
                     /locus_tag="PKH07_00590"
     CDS             613..2337
                     /locus_tag="PKH07_00590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33478.1"
                     /translation="MTKPVPSITEIPNELTSDIDLASPLGIVRQLRQSDGQIYNGYST
                     FPAISDQEILDRFETCVEMAADVLRSPEPARIVLSGAGTSGRLGMFVSRAFNRILPKS
                     KAIFRYLIAGGNLALIKAQEGAEDDPNRAVEELQSVIGEAKKVLHVGVTCGMSAPYIA
                     GQIAWCLKQPHTKSILLGFNTIDRSRNHPVEGWDRTFRDVAVEAEQSNSCVVLTPIVG
                     PEPITGSTRMKGGSATKLLLEVLFVEAARRAGLLDSAGTTKLSLIQQLSRYDDVRRSL
                     YQQERDLSELVHLGGETLRSRDENAYGHLYYLGAESAGILGLVDASECPPTFGAHFGD
                     VRGFVGGGWETLLGSGADLSDQGEWYRIGLDEFASHLLPKTTASDLVVGLGIERMGPE
                     VLSLLERAYSQGAKTAAIVVNAGEALPSWLNSAVKPEVGSTGPVDGLPAYAEYALKLA
                     LNALTTGGHVLAGKVYGNRMIDLRISNNKLFYRTLGIVTTLMGVSDEIARESILKAIY
                     ATDTPTEAMRTAPISEYVNRATPREKVVPIALLIATGQFNHAQAKEALSQNPIVRNLV
                     GQYARGKA"
     gene            complement(2371..3852)
                     /locus_tag="PKH07_00595"
     CDS             complement(2371..3852)
                     /locus_tag="PKH07_00595"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNT33479.1"
                     /translation="MEVQSKRYRVRPELCLFLLGGVLVFSCLGSKTLWTDEISIDWMT
                     QWDVATTISHCRGATGFCYSPPLGPLIVFFAKRVSDTEFMIRLPSAIVGLLLLVVFWK
                     CTSEFIPRKAALASMALMAVSPCFVYHAQDARLYILMSLCTVTSTWLMIRAVYRPVNG
                     FWPLYLLLALVNAVNLYLSYFAWLALGIMFVVFFVLLSTSAAARAESRKRKSLYFLSA
                     YALTALSYLPWLKDLLAIMQRHTSGQVAPSAVVTLPFVGNLLLELCGGGWTGVVILGC
                     VIIGAVYAAIHARVVFLCGLLSLALSLAFLAMARPSYEFSSRYFFFLHPLLMVAGGTG
                     LWCVAEMLLRKTRIPSRAIIAFLSVVIAIPSSHDLYHYYRVDRSGWKKAADFIAQNLP
                     PETLLLPGIHFSNASLNRYLIREHPTFEKVRLDPQLDYESLREVILSCPNLWYVTHAW
                     THHDPKTKKLITERLIQVADFEGYDPYRHVYIFVQQNRFRVKR"
     gene            complement(3990..5405)
                     /gene="tilS"
                     /locus_tag="PKH07_00600"
     CDS             complement(3990..5405)
                     /gene="tilS"
                     /locus_tag="PKH07_00600"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HNT33480.1"
                     /translation="MDAKRSFLQALKRHDLLLGCSRVLLAVSGGPDSMALLHLFLGIP
                     KNKRPQLEIGHFDHGLRGEDSTEDALFVQQQAALLGITCHIAHESDPPNIESSGVEQW
                     ARDIRYQWLERLAVSQNCQAVVTAHHADDQAETVLMNIVRGTGLRGLSGMPWRRPIST
                     THPEIQLLRPLLGIRRAPLLEFLQTHNIPFRTDITNDETGCKRNRTRHLVLPTIEREM
                     NPAVINNLCALAQRCQADEDFLEQMCQKTLGLLAVRTDEGVEISLPVFRSLHQAVRVR
                     VLQSLLRTLSIPSGHSIGRMLSDIELGVFSEIPQEDYSLPVGWVAQKRYDKLIIKPTS
                     DADEDYCFPVTLGVSLPLPCGMHVTCDVLSRAEWADITQKHPGQDSDHRMEGFDLRAI
                     HLPLSIRNRRQGDRMVLEGGIHKKVKDLLMDAKMPRERRRQIPLLVDAHGGILWIVGM
                     RRSAIGWVGDDTEQVLVAGVQ"
     gene            complement(5405..6181)
                     /gene="lptB"
                     /locus_tag="PKH07_00605"
     CDS             complement(5405..6181)
                     /gene="lptB"
                     /locus_tag="PKH07_00605"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HNT33481.1"
                     /translation="MQRLRAENLTKTFRGKTVVDGVSIEVNRGEIVGLLGPNGAGKTT
                     SFRMIIGLLRPERGHVYLDDLDITSWPVFRRARTGIGYLSQESSVFRGLTVEENIRAV
                     LEVQKIPRTEQKERLGELLDLIALTPLASQRASTLSGGERRRLEITRALAASPSFMML
                     DEPFSGVDPKAVSEIQTTIGKLRDLGIGILITDHSVRETLEVTNRSYIIHQGRVLTFG
                     TASELVDDPRVKEAYLGDNFYMRLGGEVRGGSTSETPPTE"
     gene            complement(6190..>7306)
                     /locus_tag="PKH07_00610"
     CDS             complement(6190..>7306)
                     /locus_tag="PKH07_00610"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNT33482.1"
                     /translation="EAFKRSISNPRRGVGFGTVEKLAEWAAQRGCSLLEACRASFDQD
                     TVRSSQKVALIRYASQVDGWRKQLEGGVPLGQVLECILNESGYRQMLLADPGEKSRLE
                     NLDAMLAGASEFEIGFLNMTRPDQEESSLIPSSVNTAQAYVERASLMSAPDALAEGTD
                     RVTLMTLHCAKGLEFPIVFLTGLEEGILPHSRCLEHAASLEEERRLCYVGMTRAKERL
                     YLSFAWDRKQRFSVNRSVSQTVSRFLLEIPEQFFDARSRDVYRTIKEPWRRFAQTDFA
                     TEQRESFSARKTPSKDSSFGTWKPRPPVPPVAQKVAAGERVASLSVGDRIAHPSFGVG
                     VVQLVRGSGENVLATVRFQDGETKVIMPKFARLKKLK"
BASE COUNT         1726 a         2027 c         1930 g         1623 t
ORIGIN      
        1 cggctctgtg acgacgcctt gggtcgggaa gacctgctca cattggcgac ggagatagaa
       61 ggtcatcctg acaatgtttc tccggcgctg tgtgggggct tgacaatcag ctacatggca
      121 gacagggaag tcgtggtttg tccttgccga atctccatgc ccttgaaagt ggcggttgtt
      181 ctgcccgcag ccaagaagga gagcacgtca cagacccgaa aattgtttcc gcgcaaagtg
      241 gatcttgcgg atgcgctcca caaccagagt cggactgccg ccattgtcgc aggacttctt
      301 cagggcgact tgaagcaagg acggacgttg tttgacgatc gactgcacca aagacagcgc
      361 tgtgttctga tgccctggtt gttccacgca atcgaggccg cctatgatgc cggtgcactg
      421 ggtgcctttc tcagcggagc aggacccgcg gtcgctgccc tctgccggac ggaagatctt
      481 gctgtcgttt tggaagccat ggatgctgcc atctccgctc aaggaatgcc gaaccgtcgc
      541 tttgggttgg atattgccgc ggagggggcg gaattccttt cggtgcgctg aagagaacaa
      601 ggaagtgaga acatgaccaa accagtaccg tccatcacgg aaatccccaa tgagttgacc
      661 tcagacattg atctcgcttc tccgttgggg atcgtccgtc agctgcgtca gtcggatggc
      721 cagatctaca atggatattc gacatttccc gcgatctcag atcaggaaat ccttgatcgg
      781 ttcgaaacct gcgtcgaaat ggcggctgac gtgctcagaa gtccagaacc ggcccgaatt
      841 gtgctgtccg gtgcgggtac cagcggacgg ttaggcatgt ttgtttcgcg cgctttcaac
      901 cgcatccttc ccaagtccaa agcgatcttt cggtatctta tcgcaggtgg aaaccttgct
      961 ttgatcaaag cccaggaagg tgcggaagac gaccccaatc gcgccgtaga ggaactgcag
     1021 tcggtcattg gagaggccaa gaaagtactg cacgttggcg tcacctgtgg catgtccgct
     1081 ccctatattg cgggacaaat cgcatggtgc ctcaaacagc ctcataccaa gagcatcctg
     1141 ctcggattca acaccatcga tcgttccaga aatcatccgg ttgagggctg ggatcggaca
     1201 tttcgggacg tggctgtcga agcggagcaa tcgaacagtt gcgttgtgct cactccgatt
     1261 gtaggacccg agccgatcac gggttccact cgcatgaaag gcggctcggc gaccaagctg
     1321 ctgctggaag tgctctttgt ggaagcggcg agacgtgcgg ggcttctgga ttcagcgggt
     1381 acaacaaagc tgtcgctgat tcaacagctg tcacgctacg acgatgttcg aaggagtctc
     1441 taccagcagg aacgcgattt gtcagagttg gttcatctgg gtggagaaac gcttcgctcc
     1501 cgcgatgaga acgcctatgg tcacctctac tatctgggtg cagagtccgc cggtatcctt
     1561 ggccttgtgg acgcttcgga atgcccaccg acgtttggag ctcacttcgg cgatgtgcgc
     1621 ggattcgttg ggggtggatg ggaaactctt ctcggttcgg gagcggactt gagcgatcag
     1681 ggggaatggt accgaattgg tctcgatgaa ttcgcttctc atcttcttcc gaagaccacc
     1741 gcatccgatc tcgttgtcgg acttggaatc gagcgaatgg gacccgaagt cctaagcctg
     1801 ctcgagcgag catactccca aggagccaag accgcggcca tcgtcgtgaa tgcgggggag
     1861 gcactgccct catggctgaa cagtgcggtg aagcctgaag tcggctccac aggtcccgtg
     1921 gatggccttc cggcctatgc cgaatatgcg ctgaaacttg ctctgaatgc tctgaccacc
     1981 ggtggccacg tcctggcagg aaaagtctac ggcaaccgga tgattgatct gcgcatcagc
     2041 aacaacaaac ttttttatcg aacgttgggg attgtcacca cgctgatggg tgtctccgat
     2101 gagattgctc gggagagcat tctgaaggca atctacgcca ccgacacacc caccgaggca
     2161 atgcgaacgg ctccgatttc cgaatacgtc aatagggcta caccccgtga gaaagttgtt
     2221 cccattgcgc ttctgattgc caccggacag ttcaatcatg ctcaagccaa agaggcgctt
     2281 tcgcaaaatc ccatcgtccg aaaccttgtt ggccaatatg cccgcgggaa ggcatgagcc
     2341 ggtgctctct ccgactcgct gcatttcagc ctaacgcttg acgcggaatc ggttctgctg
     2401 cacaaaaata tacacatggc gatagggatc gtagccctcg aaatccgcca cctgtatgag
     2461 tctttctgtg atcagtttct ttgtctttgg atcatggtgc gtccacgcat gggtaacata
     2521 ccagagattt ggacagctga ggatgacttc ccggagactc tcataatcga gctgcggatc
     2581 gagtctcact ttttcgaaag tcggatgttc tctgatcaaa tacctgttca aggaagcatt
     2641 cgagaagtgg atcccaggta gcagaagagt ctccggtggc agattctgag caatgaaatc
     2701 tgccgccttc ttccacccgc ttctgtcaac acgatagtag tggtacagat cgtgactgga
     2761 cgggatggcg attacaacag agagaaatgc gatgatcgcg cgtgagggaa tcctggtctt
     2821 tcgtaagagc atttctgcga cacaccacaa ccccgttcct cccgcaacca tcagaagtgg
     2881 atgcaaaaag aagaaatatc ggctgctgaa ctcgtagctg ggtctggcca ttgcaaggaa
     2941 cgcaagactc agggcaagac tcaacaggcc gcacaagaac acaaccctgg cgtgtatcgc
     3001 tgcgtagact gctccaatga tcacacatcc cagaatcaca acacccgtcc atccacctcc
     3061 acagagttcc aggagaaggt ttccaacgaa aggcagagtt acaacagcag aaggagccac
     3121 ctgtcccgaa gtgtgtcttt gcattatggc caagagatcc ttcaaccaag gaagatagct
     3181 tagagcagtt agggcgtatg ctgagagaaa gtagagactc ttgcgctttc ggctttctgc
     3241 tcttgccgcc gctgaggtgg acagcaagac aaagaagact acaaacatga ttcccagagc
     3301 caaccacgcg aagtaactga gatacaggtt gacggcgttt accagagcca gcagcaagta
     3361 cagaggccag aaaccgttga cgggtcggta tactgccctg atcatgagcc atgtcgaagt
     3421 gaccgtgcag agactcatca gaatatacaa tcgcgcatcc tgagcgtgat agacaaagca
     3481 gggcgacaca gccataagag ccatacttgc caacgccgcc ttgcggggaa tgaattctga
     3541 agtgcatttc cagaaaacga caagaagcaa cagtcccact atggctgaag gtagccgaat
     3601 catgaactca gtatccgaga cgcgcttggc aaaaaagacg ataaggggac ccaatggggg
     3661 agaatagcag aatcctgttg cacctcggca atggcttatc gtagtcgcca cgtcccattg
     3721 cgtcatccaa tctatggata tctcatctgt ccagagagtc ttggaaccaa ggcacgaaaa
     3781 gacaagtact ccaccaagaa gaaataaaca aagctcaggt cggactctgt agcgcttgct
     3841 ctgaacttcc atagagttga cactcactcc aagaaccggg cgcgtcatgc aaacaacacg
     3901 ctctgccgat cagggaaatc tctctgatag cagagccgtt gcattccctt gccactcgcg
     3961 gtctcttctc ctcatgcctt cgatggtctt cactgcaccc ccgcgaccag cacctgttcc
     4021 gtgtcgtctc ccacccagcc gattgcagac cgacgcatcc caactatcca gaggattccg
     4081 ccatgagcat caaccagaag cggaatctgc cgtcgccgtt cacgcggcat cttggcgtcc
     4141 atgaggagat ctttcacttt cttatgtatc ccgccttcga gaaccatccg gtcgccttgt
     4201 cttcggtttc tgatgctgag cggaagatga attgctctca ggtcaaatcc ttccatccga
     4261 tgatctgaat cctggcccgg atgcttttgt gtgatatctg cccattccgc cctggaaagc
     4321 acatcgcagg tgacgtgcat tccgcagggc agtgggagtg acacgccaag ggtaactgga
     4381 aagcaataat cctcatcagc gtcgcttgtc ggtttgatga tcagtttgtc atatctcttc
     4441 tgagcgaccc atccgacagg gagcgagtag tcttcctgtg gaatctcgga gaaaacaccc
     4501 agttcgatgt ctgagagcat tctgccgatg gagtgtccag aaggaatgga caacgtgcgc
     4561 aaaagagact ggagtacacg cacccgaact gcttggtgca gactacgaaa aacaggcagc
     4621 gatatttcca caccttcgtc tgttctgaca gccagaagac ccagcgtctt ctgacacatc
     4681 tgctccagaa aatcctcatc ggcctgacag cgttgagcca gtgcgcatag attgttgatg
     4741 actgccgggt tcatctcccg ctcgattgtt ggcaagacaa gatgccttgt gcgattgcgt
     4801 ttgcagccgg tttcgtcgtt tgtgatatcg gttctgaatg ggatattgtg agtctgaagg
     4861 aactcaagga gaggggcacg acgaataccc aagaggggac ggagaagttg gatctccgga
     4921 tgtgtggtgc tgatgggacg ccgccaaggc attccggaaa gaccgcgaag ccctgtgcca
     4981 cgaacgatgt tcatcaaaac cgtctccgcc tggtcatccg catgatgtgc cgtgacaacg
     5041 gcctgacagt tctgactgac agccagccgt tcaagccatt ggtagcgaat gtctctcgcc
     5101 cactgctcga ctccggaaga ttcgatgttc ggcggatcac tctcatgcgc aatgtgacag
     5161 gtgatgccaa ggagtgcggc ctgctgttgc acaaaaagcg cgtcttcggt tgaatcctca
     5221 ccgcgcagac cgtgatcgaa atggccaatc tccagttgag gtcttttgtt tttgggaatt
     5281 ccaagaaaga gatgcagaag tgccattgag tccggcccac ctgaaacggc caacaaaact
     5341 cggctgcagc ccaacagcag atcgtgtcgc ttcagagcct gcaggaatga cctcttggcg
     5401 tccattattc cgtgggtgga gtctccgatg ttgatcctcc gcgcacctcg cctcccagtc
     5461 gcatgtagaa gttatctccc aagtaggctt ccttgaccct cggatcgtcc accaactcgg
     5521 aggccgttcc gaatgtcaac acccgtcctt ggtgaatgat ataggatcgg ttggtgactt
     5581 ccagcgtttc gcgaacggaa tgatcggtga tcagaatgcc gattcccaaa tcccgaagtt
     5641 tcccaatggt tgtctggatc tcggataccg ctttgggatc aactcctgaa aaaggctcat
     5701 ccagcatcat aaaactgggt gaagcggcca gagctcgggt gatctcaagc cgtcgccgct
     5761 ctccacccga gagcgtagaa gcccgctgac tggcgagtgg tgtcagcgcg atcaagtcca
     5821 gaagttcacc caagcgttcc ttctgttccg ttcgtgggat cttctgaacc tccaaaacgg
     5881 cacggatgtt ctcctcgaca gtcagtcctc gaaacacaga agattcctga gacaaataac
     5941 cgatgccagt gcgcgctcga cggaacaccg gccaacttgt gatgtccaga tcatccaaat
     6001 agacgtgtcc acgctccggc cgaagcaaac cgatgatcat tcgaaatgaa gtggtctttc
     6061 cagcgccatt gggacccaga agaccgacaa tctctccccg gttgacttcg atggagacac
     6121 cgtccacaac ggtctttccc cgaaaggtct ttgtcagatt ttccgctcgc agcctttgca
     6181 tgctctctcc tacttcaact tcttcagtcg cgcgaatttg ggcattatga ccttggtctc
     6241 cccgtcctga aatcgaacgg tggccagtac attttctcca gagcctctta ccaactgaac
     6301 gacgccgaca ccgaaactcg gatgagcgat gcgatccccg acagacaggg atgcaacccg
     6361 ttctccggcg gcgacctttt gcgcaaccgg tggcacagga ggtctcggtt tccatgtacc
     6421 aaacgaagaa tctttcgacg gagtcttcct tgcagagaag gattctctct gttctgtggc
     6481 gaaatcggtc tgagcaaacc ttctccaagg ctctttgatt gtccgataga catctctact
     6541 tcgagcgtca aaaaactgct cgggaatctc aagcaagaaa cgcgaaacag tctgtgacac
     6601 gcttcggttg acgctgaaac gctgcttccg atcccaggca aaggagagat agagtcgctc
     6661 ctttgctcgt gtcattccaa cgtagcacag acgtcgctcc tcttccagcg acgctgcgtg
     6721 ctccaggcaa cggctgtgcg gaagaattcc ctcttccaaa cccgtcagaa agacaatggg
     6781 gaactccaat ccctttgcgc agtgaagcgt catcagtgtg actcgatcgg tgccttccgc
     6841 cagggcgtcc ggtgctgaca tgagtgatgc ccgctcaaca taggcctgcg ccgtgttgac
     6901 agaagaagga atgagactgc tctcttcctg gtccggtcgg gtcatgttca ggaatccgat
     6961 ctcaaactcg gacgctccgg caagcatcgc atcaaggttc tccagccgcg acttctctcc
     7021 tggatcggcg agcagcatct ggcgatatcc ggattcattc agaatacact ccagcacttg
     7081 cccaagcgga acgccgcctt ccaactgttt tctccaacca tccacttgtg aagcatatct
     7141 tatcagcgca accttctggc tggaacgaac cgtatcctga tcaaaagacg cccgacaggc
     7201 ttccaaaaga gagcatcctc tctgtgcagc ccattcagca agtttctcaa cggttccaaa
     7261 gccgacgccc cgtcgaggat tgctgatact gcgcttgaaa gcctca
//
LOCUS       DAOQUC010000018         7181 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_11485_length_7181_cov_3.217373, whole genome shotgun sequence.
ACCESSION   DAOQUC010000018 DAOQUC010000000
VERSION     DAOQUC010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7181
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11485_length_7181_cov_3.217373"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            395..663
                     /locus_tag="PKH07_00615"
                     /pseudo
     CDS             395..663
                     /locus_tag="PKH07_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008868900.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            676..1011
                     /locus_tag="PKH07_00620"
     CDS             676..1011
                     /locus_tag="PKH07_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020878479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HNT33483.1"
                     /translation="MRRLAPVTPGEILREEFLVPMGISQYRLAKEIGVPAQRISEIVA
                     AKRAITPDTDLRLCRFFGLSNGYWLRAQAAYETETAEAILAETLAKIKPWQGHGLQTR
                     ATGKRKQQA"
     gene            complement(1023..2645)
                     /locus_tag="PKH07_00625"
     CDS             complement(1023..2645)
                     /locus_tag="PKH07_00625"
                     /inference="COORDINATES: protein motif:HMM:NF012509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase family protein"
                     /protein_id="HNT33484.1"
                     /translation="MAAEQWLDRFALADPTQESSLRSIYGVDRDIILERVNLFGTVLA
                     TFCHAFGARQPVFLVRCPSRVNWEGHHVDHQGGSYNATTQAREFVAACSERQDGWVIV
                     HNAESLRFRPCRFRVDDLSPQHHRSGGWERFVIGAWIALKERFPTLVFHGVNLALGSD
                     IPTGGGMSSSHALLVTSLLAAATANRLTLQRSEIVSLIQRADWLAGSRTGLGDQATML
                     FGRRGFLFHSPVGTADEISPEYIPLPDGYALMIADSLTHHTLAGQEAVAYNSRVFACR
                     LALPLLLQGFAALGIDETTIASLRCPADISPQKVPLELIYQGLGAIPSDLRLDEARLR
                     FQEACVSGLTSLSFDELVETYFPSGERPDTVPVRGVLMYTLAECRRSVLFAQHLLEGN
                     ISEAGRLMNAGHEGDRVSRANPETGEYTNAEYPVTDILLKDLLDDLRSQEPNRTDRAQ
                     LQYQHGDYRASTPTLDQMVDIARTHGAVGASLTGGGHGGVMIALMQEDRCDGFREAMC
                     FFHRERSCLSEDQASRTVQKCVTVEGAGMISI"
     gene            2768..4045
                     /locus_tag="PKH07_00630"
     CDS             2768..4045
                     /locus_tag="PKH07_00630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33485.1"
                     /translation="MQKEKEAETLRLPFSLVILALAAVFLVALVPRILYVIQIQTPPF
                     SDMADYHRCAVNFMLGDGLIQSPEYKAYRTPGYPIFLATIYSLFGIDFQVVYLVQCVL
                     GALTAVLTALLFLKCFPGIRYRLLLATLAGLITALSDESIFYCGQLLTETLYTFLFVI
                     WVLLLMHVQRGFRLTLLVIVGLLHGLLMLVRPAGSLHVIMVGVFLVLWARRKDSRRSV
                     REGLISYIIYSAVVLVPIVPWGVRNYLLTGHLIPLSTNDGVNFYIGHNPDFGYWSTGN
                     KTAIRENTDLDEYEESRLFFRLGLEFILKNPVQDMVNNIKKIGYLYTTTWKPWPWFNH
                     GRELRFAFVRWMPVWEWSWPAMVVIGLGSVASTLKRQKTDLLLGCVITQTISCMVFFA
                     TARFRSSLIPLFAIWVAYAFFVLISTVRQRGNP"
     gene            complement(4116..6395)
                     /locus_tag="PKH07_00635"
     CDS             complement(4116..6395)
                     /locus_tag="PKH07_00635"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HNT33486.1"
                     /translation="MSNQSEDIIAHNVESLLAKTAFATRGKHAKPIADATEPVPSEIA
                     HHPEQPTTIPSSAIQSIEDRITHIENRLDDIRLKSTALIAAQKKQIDQLQSAGQQRSQ
                     RLHEFEDSFDQKYQSFVNATTNLAERIAQVSEQIPSFLDNQGQMSDRISGIQAGFALL
                     QESSQEIRSALERQATETSEQLRTLKTEIASVTSQYQEPLRRLQEQLDDLSSLMTKQD
                     AQTQTRLATFDTEQNAHRNELDNIIAQLSRGAEERNAQIKTLQDAFAALEAGLSRTNN
                     QVNSNEKAITELVKHAEEEDKRSASLAQDTQAELSRITAQVNSNEKTITELVKHAEEE
                     DKRLEAFGEHLKSAYSDTQETALRNRQSIEELTRHAQKCDALIKALGVQQQQDRESLT
                     QAAADIAKDREKALEAIGIRMDASDATIQGVKDFLEALITERTENLARHLEANESALQ
                     IWSALVTELENRGRVQDDRIQEIKAQSQAILADLESLKAKDTVGQDQLVRVQEQVRAI
                     QMQFEAIQADKSHRDQLREASLSPSDYFVFEDRFRGNEADVQRNQSRFLTLFKGCHHV
                     LDIGCGRGEFLDLLIQSGIGASGIDMNPVMVEYCQKRGLRVAQADAFEFLENVEDALL
                     DGVYCGQMIEHLTAEEQIAFVKLVYRKMRFNSYFVIETMNPMNLPTAATHFYTDMSHK
                     RPVTPDGLGFLLSAVGFRKVQFDSLSETPQSEKLTPIALGTKPSPATRKLVEALNQNL
                     DKLNCLLFAYRDFAAMALK"
     gene            complement(6388..>7181)
                     /locus_tag="PKH07_00640"
     CDS             complement(6388..>7181)
                     /locus_tag="PKH07_00640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33487.1"
                     /translation="PAVPSSQIESMHLDELLRQINQEWDLTRYSLESKRRNFAQVVTG
                     FKKMLDVVLKPYANIFLYQQATYNSHVVQILNLLGQKLGRVESFLAEIANEQTALRVV
                     LEGKVDEINRKLDLLGADFLHKHEQTQAALAEGFDRSQQGLEAQRQHFEQAHAHLREE
                     VGSQQENTRQRMEQGFAQSQSLLEQQKQYMDQVQNQIMDDLADGLRISLTEVAQRLQA
                     ISSLAEKNQKQLEEKFERNMDALLALLTEERQGTTDNTVHDSQNE"
BASE COUNT         1550 a         1924 c         1874 g         1833 t
ORIGIN      
        1 taaatgccgc cagaaggagc aacgccagtg cccaaagcat tttgttaagc cttgcgtttc
       61 ccgaaatcgc cggctcttgc ttgacgagat taaccgtctc catgatggaa cctctctgga
      121 atccacattc ggtcctactc gcagaaccat ctcattagaa accccattgt ctgatatcaa
      181 ccgcgtgaca ctaactacta ccatacagct ctgtatcgcc cctgttcaac aggaatctct
      241 gctttcacag taacttgtct gtctctgctg tcttgccagg atggttagcg tacaggctgg
      301 aagactgtgc agcataagag atatccgaac ggcaagtttc caaaacggtg ggaccagtgt
      361 tgacactagt cacgcatcgc attactattt ctctatgatt cggtcatttc ggtgcactga
      421 tacgaaggct ttggcgcgag gacaacggat caaacgatcg agacagttgc gagaaggaag
      481 atccgtcaga tcgagattgc ggagcggctc gatgacctta gagttcctcc aggcaaccga
      541 ctcgaacccc tcaaaggcga cagagacgga caatacagca ttcggatcaa cgatcagttc
      601 cagctgtgtt ttcgctggat agatatgtgc gcgcaggatg tcgaaattgt tgactatcat
      661 tgaaggagaa tcactatgcg aagacttgct cccgttacac caggtgagat tctgcgtgaa
      721 gaattccttg ttcccatggg catttcacaa tatcggttag caaaagagat tggcgttccc
      781 gcacagcgga tcagcgagat cgtggcagcg aaacgtgcca tcacacctga cacggatttg
      841 cgactttgtc ggttcttcgg cctctccaat gggtactggc ttcgagccca agcagcctac
      901 gaaaccgaga ctgcagaggc tattttggcc gagacgcttg ccaagataaa gccctggcag
      961 gggcacggtc tacagacgcg tgcgacagga aagagaaagc agcaagcata gcctctcact
     1021 ccctatattg agatcatgcc cgccccttca accgtcacgc atttctggac tgtccgtgac
     1081 gcctgatcct cagaaaggca gctgcgttcc ctgtgaaaaa aacacatcgc ctcacggaac
     1141 ccatcgcatc tgtcttcctg catcaatgca atcatcacac cgccgtggcc accgccagtc
     1201 aatgacgcac caaccgctcc atgggttcgg gcgatgtcca ccatctgatc cagcgtcggc
     1261 gtgctcgcgc ggtagtcgcc atgctggtat tgcagttgtg cacgatcagt tcgatttggc
     1321 tcttggcttc tcaaatcgtc caacaaatcc ttgagaagaa tatcggtaac tggatattca
     1381 gcgttggtat attccccagt ctcaggattg gctcttgaga cccgatcgcc ttcatggcct
     1441 gcgttcatca gtcgtcctgc ttcggaaatg tttccctcaa gcagatgttg ggcgaataga
     1501 acgctgcggc gacattccgc cagggtgtac atcaagacgc cacgaacggg aaccgtgtca
     1561 ggcctctcgc ctgatgggaa ataggtttca accagttcgt cgaaactcag gctcgtcaag
     1621 ccactgacgc aagcctcctg aaatctcagc cttgcttcat ccagtcgcag gtcgcttggg
     1681 atcgctccaa gtccttgata gatcagttcg aggggtacct tctgcggtga aatgtcagca
     1741 gggcatcgga gagatgcaat ggttgtctca tcaatgccaa gagcagcgaa gccctgaagc
     1801 agcagtggca aagccagtcg gcaggcaaag actctgctgt tgtaggcgac cgcctcctgt
     1861 cctgccagcg tgtggtgcgt caacgagtcc gcaatcatca gcgcataacc gtccggcaga
     1921 ggaatatact cgggtgagat ctcatctgcg gtgccaaccg gtgaatgaaa aagaaagccc
     1981 ctacggccaa agagcatcgt tgcctggtcg cccaaccccg ttcttgaccc tgccaaccag
     2041 tccgcccttt gaataaggga gacgatttct gagcgctgaa gtgtcagcct gttcgccgtc
     2101 gcggccgcca agagagaagt gacaagcaaa gcgtgcgaag agctcatgcc tcccccagtg
     2161 gggatatcgc ttcccaaagc cagattcacg ccatgaaaca ccagcgtggg aaaccgttct
     2221 ttgagtgcta tccaggctcc gatcacaaag cgttcccatc cgccgctcct gtggtgctgc
     2281 ggggaaagat catccactcg aaatcggcat ggccgaaacc gaagcgattc ggcattgtga
     2341 acaattaccc agccatcctg acgctcggag caggctgcga caaactcccg cgcctgtgtt
     2401 gtggcgttgt agcttccgcc ctgatgatcc acgtgatgcc cttcccagtt gactcgggac
     2461 ggacaacgca caagaaacac cggctggcgc gctccgaaag catggcagaa cgtggcaagc
     2521 accgttccaa agaggttcac tcgctccagg atgatatcgc gatctacacc atagattgac
     2581 cgcagtgaag actcctgggt gggatctgca agagcaaaac gatccagcca ttgctccgca
     2641 gccaaggcaa agggttctga tgggaatggg ttttcgatga tcggctttcc tttccacagc
     2701 caactctggt atcgtcaaca ctatgatcga ggctgatatt cgtcaagatg aaccaggaag
     2761 cgatcctatg caaaaggaga aggaagcgga gactctgcga ttgccgtttt ccctcgtgat
     2821 ccttgcgttg gctgctgtgt ttcttgtcgc gctggttccc agaatcctct atgtgattca
     2881 gattcagacg cccccattct ccgacatggc tgactaccat cgctgtgccg tgaatttcat
     2941 gttgggcgat gggctcattc agagtccgga gtacaaagcc tatcgaactc cgggctaccc
     3001 gatctttctg gcgacgatct actctctgtt tggcattgat ttccaagtgg tctatctggt
     3061 tcagtgtgtt ctcggtgcgc tgacggcagt cttgaccgct ctgttgttcc tcaagtgctt
     3121 cccaggcatt cgataccggc tgttgttggc gacgctggca ggactgatta ccgcattgtc
     3181 ggacgaatcc atcttctact gcgggcagtt gcttaccgag actctctaca ctttcctttt
     3241 cgtcatctgg gttctcctgc tgatgcatgt acagcgcgga ttccgactga ctctgttggt
     3301 gatagtcggg ctgttgcacg ggctcttgat gctggtcaga cctgctggca gtctgcacgt
     3361 catcatggtt ggagtttttc tggtactctg ggctcgtcga aaggattcgc ggcgatccgt
     3421 cagagagggt ctcatctcgt acatcatcta ttctgcggta gtcctggtgc caatcgtccc
     3481 ctggggtgtt cggaactatc tgttgaccgg tcatctcata cctctgtcca ccaatgatgg
     3541 agtgaacttc tacatcggac acaatcctga cttcggttac tggtcaacgg gcaacaagac
     3601 agcaattcgc gagaacacgg acttggatga atacgaggaa tccagactgt ttttccgttt
     3661 gggtctggag ttcatcctca agaatcctgt gcaggacatg gtcaataaca tcaagaagat
     3721 tggctatctg tacacgacaa cctggaaacc atggccgtgg ttcaaccatg gtcgggagct
     3781 gcgctttgcc tttgtccggt ggatgcccgt ttgggagtgg tcatggcctg ccatggttgt
     3841 gatcggactc ggttccgttg cctcaacctt gaagagacag aaaaccgatc tgcttttggg
     3901 ctgcgtcatc actcagacga tttcgtgtat ggtgttcttt gccacggcgc gttttcggtc
     3961 atccctcatt ccgctgtttg cgatatgggt agcctacgcc ttcttcgtgc tcatctcgac
     4021 agtacgtcag agaggaaacc cctgaaatat catcttgctc gagcagatgc gtcaagattc
     4081 ggctctgttg cacagcaggg gaaaggtttc tggaactact tcagcgccat ggcagcgaag
     4141 tcccgatacg cgaagagaag gcaattgagt ttgtcaagat tctggttgag tgcctcgacc
     4201 agttttcgtg ttgctgggct cggcttggta cccagtgcga tcggagtcag tttctctgat
     4261 tgaggtgttt cggagaggga atcgaactgc accttgcgaa atccgaccgc gcttaaaagg
     4321 aacccgagtc catcgggtgt gaccggtcgc ttgtgcgaca tgtctgtgta gaaatgtgtt
     4381 gcagccgtgg ggagattcat cggattcatg gtctcaatga caaagtagct gttgaagcgc
     4441 atctttcggt agaccagttt cacaaatgcg atctgctctt cggcggtcaa gtgctcaatc
     4501 atctgtccgc agtagactcc atcgagcagg gcgtcttcga cgttctcaag aaactcaaaa
     4561 gcatcggcct gagcaacccg aagtcccctc ttctgacagt actcgaccat gactggattc
     4621 atatcaattc cggatgcacc aataccggac tggatgagca aatcgagaaa ctctccccga
     4681 ccacacccga tatccagaac gtgatggcac cccttgaaga gggtcaggaa tcgtgactga
     4741 ttcctctgaa catcggcttc attcccgcga aaacggtctt caaaaacaaa gtaatccgat
     4801 ggggacagtg aagcctctcg aagttgatcg cgatgagact tgtccgcctg tatggcttca
     4861 aactgcatct ggattgcacg gacttgttcc tgaacccgaa cgagttggtc ttgcccgaca
     4921 gtatctttcg ccttgaggct ctcgagatcc gccagaatcg cctgactttg agccttgatc
     4981 tcctgaatcc ggtcgtcctg tactctgcca cgattttcca actcagtgac caaggcagac
     5041 caaatctgaa gtgccgactc attggcttcc aaatgccttg ccagattttc tgttcgttcc
     5101 gtaatcagcg cttcgagaaa atccttgacc ccctgaatcg tcgcgtcgga agcatccatc
     5161 ctgatgccga ttgcttccaa ggccttctct cgatccttcg caatgtcggc tgcggcctga
     5221 gttagactct cacgatcctg ctgctgctga acaccaagtg ccttgatcag cgcgtcgcat
     5281 ttctgagcat gtctcgtcaa ttcctcaatg gactgccgat tgcggagcgc ggtttcctga
     5341 gtgtcggaat aagcagactt caagtgttcg ccgaatgctt ccaagcgttt gtcttcttcc
     5401 tcggcgtgtt tcaccagctc ggtaatcgtc ttttcattgc tgttgacttg agcagtgatg
     5461 cgcgagagtt cggcctgagt gtcctgggca agtgatgcag agcgtttgtc ttcttcctcg
     5521 gcatgtttca ccagctcggt aatcgccttc tcgttgctat tgacttgatt gttcgttcgc
     5581 gaaagtcctg cttctaatgc ggcgaaggcg tcctgcagcg tcttgatttg agcatttcgt
     5641 tcttcggcgc cccgactcaa ttgggcgatg atgttgtcta actcatttcg gtgagcgttc
     5701 tgctcagtat cgaaagtcgc caatcgcgtc tgtgtttgtg cgtcctgttt tgtcatcagg
     5761 ctgctgagat cgtccagttg ttcctggagt ctgcgaagtg gctcctgata ctggctggtc
     5821 actgaagcga tctcggtctt gagggttcgg agttgttctg acgtttcagt ggcctgtctt
     5881 tcgagcgcag atcgaatctc ttgggaagac tcctgaagaa gggcaaatcc cgcttggatg
     5941 ccggagattc ggtcgctcat ctgaccctgg ttgtccagaa aggacggaat ctgttcgctg
     6001 acctgggcaa tgcgctcggc aagattggtt gtggcgttta caaaagactg atacttctga
     6061 tcaaaactgt cctcgaattc atgcaggcgt tggcttcgct gctgtcctgc cgactgcagt
     6121 tgatcgatct gcttcttttg ggctgcgatc agtgcagtgc tcttgagcct gatatcgtca
     6181 agacggtttt caatgtgggt aattctgtcc tcgatgctct gaatggccga ggagggaatc
     6241 gtggttggtt gttccggatg atgtgcaatc tctgaaggga cgggctctgt cgcgtcagca
     6301 atgggtttgg cgtgtttgcc cctggtcgca aatgcagtct tcgcgagcag cgactcgaca
     6361 ttgtgggcga tgatgtcctc gctttggtta ctcattttgg gaatcgtgta ccgtgttgtc
     6421 tgtggtaccc tgacgttcct cggtcagcag tgccagcagc gcatccatat ttcgctcaaa
     6481 tttctcttcc agttgtttct gattcttctc cgccagtgag gaaattgcct gaagtctctg
     6541 ggcaacctca gtcaaagaga tcctcagacc atcggcaaga tcatccatga tctgattctg
     6601 cacctgatcc atgtattgct tctgctgctc cagcaaagat tgagactgag cgaagccctg
     6661 ctccatcctc tgacgggtgt tctcctgctg cgatccaact tcttccctca gatgagcgtg
     6721 tgcctgttcg aaatgctggc gttgtgcttc caaaccttgt tgagatcgat caaacccttc
     6781 cgcaagagcg gcttgtgtct gttcatgctt gtgcagaaaa tccgctccca gaagatcgag
     6841 tttgcgattg atctcatcca ccttgccttc cagcacgacg cgaagtgcgg tctgctcgtt
     6901 ggcaatctcg gcaagaaagg attccaccct gcccagtttc tggccaagca gatttagaat
     6961 ctgaacgaca tgcgagttat aggttgcctg ctgatagaga aaaatgttgg cgtagggctt
     7021 cagaacgaca tcaagcatct tcttgaagcc ggtaacaacc tgggcgaagt ttcgccgttt
     7081 ggactcaagg gaatatcggg tcaagtccca ctcctggttg atctgccgta gcaactcatc
     7141 caggtgcatg gattcgatct gggaagacgg caccgccggt g
//
LOCUS       DAOQUC010000019         7097 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_11667_length_7097_cov_3.238427, whole genome shotgun sequence.
ACCESSION   DAOQUC010000019 DAOQUC010000000
VERSION     DAOQUC010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7097
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11667_length_7097_cov_3.238427"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1448)
                     /locus_tag="PKH07_00645"
     CDS             complement(<1..1448)
                     /locus_tag="PKH07_00645"
                     /inference="COORDINATES: protein motif:HMM:NF013647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HNT33488.1"
                     /translation="MFLAEPMSELSVVFLQQDEAQIIKLASESGVLHMIEPQLMHTWT
                     KELHGEDIAVVERQYGAISAKARSLLQDMGKLIPLPTHLPPESLKNPDELQKSLNEIS
                     APVRKIQARLESLNTEKSQLDLLSRQLHSPRAGEIAEAFAGPHSFLKVSFASGREANL
                     ATLQSRLEGVPSVVMPLSVEDERVTILAMVLRRDRVALDKALQAAGFEAEDPSRLVPQ
                     HEISLEKLQDKRTEIEQELASQRTDLEKWAAENTERAIQIFYQARRGEMAAKARSFVR
                     ATRHARFLCGWVPTGKGRSLIREIQQSTQGRAFTELVSADQVEGVRTRRADVPALFRN
                     PGWMRPFEPLTTGFGIPTYRSVNPTPFLAVTFLFMFGMMFGDVGHGLVLALGGWFLSR
                     RNATRNLGFVLLMAGASSILFGMFYGSVFGLEHVIPAMVLHPMEEIETLALIAIVFGV
                     LMISLGVILRIINASREGNWADVAFDKSGLISG"
     gene            complement(1470..2522)
                     /locus_tag="PKH07_00650"
     CDS             complement(1470..2522)
                     /locus_tag="PKH07_00650"
                     /inference="COORDINATES: protein motif:HMM:NF014094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HNT33489.1"
                     /translation="MLGGLSQYAFLNAKVRGMMSRLLDEEQIRALANCADLDHALVVL
                     KDTPYGYLVTQLSETPRLSELERLLLLDEAAAYREIAGLTKGSVEQVFEILESWYELE
                     DLKGALRAWVRSRAGAPSDFMPLGVGDLPLEEILQAESLEQVALLLKGTPYGSTLLSA
                     SPLFQSRKSVFVLEIALDQSYYRRLWSSLETLSERDRRVASRLVGIEVDLKNLQWILR
                     GIMYYRIPTAELLEDLIPHGWRIQPSVTRKALEKSDVTDVLEQLALGPYQELLSGWLA
                     RAPRESAALLEGILWEILLRQVRLSLAGFPFTIGTPLAYCFLKRLEVQNLIRLLESIA
                     MEIPPDEIGGFPVNVR"
     gene            complement(2530..2868)
                     /locus_tag="PKH07_00655"
     CDS             complement(2530..2868)
                     /locus_tag="PKH07_00655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33490.1"
                     /translation="MVERARQLIEDILKKEKAASDSLRQATDEARQIVSEAEREAKEQ
                     IASGEKHAQQAARELVVQQSLLAQEQAETEAGNAQRELSEMERALTEKLPEVVENLIQ
                     SLTTFTTTEE"
     gene            complement(2953..3564)
                     /gene="rdgB"
                     /locus_tag="PKH07_00660"
     CDS             complement(2953..3564)
                     /gene="rdgB"
                     /locus_tag="PKH07_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HNT33491.1"
                     /translation="MSTECSLLLATRNQGKRRELETILKGLPCRIVTLEEFPDAPEVV
                     EDRSTFLDNARKKAQTLARHTGLLTLAEDSGLEVDALDGRPGVYSARYARGEGSTDAE
                     NIERVLLELQGVPLAKRTARFVCQAVLYESDKEIGQAFGEVCGLISENRRGHEGFGYD
                     PIFYLPEYGKTFGELPASVKNRISHRSGALRVIRVILEGRFRR"
     gene            complement(3564..4469)
                     /locus_tag="PKH07_00665"
     CDS             complement(3564..4469)
                     /locus_tag="PKH07_00665"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNT33492.1"
                     /translation="MIRFFFRLAVAILSRLPRRVQWAFAQLLGCLGFLFAARRRRIAL
                     ENVEAAFPELSHEQRQRIVKQSLASLAMTAFEFVSLPRFRGRLSQLVEVIGEENLPAL
                     RNTPQGAILLVAHLGNWEVGGAILAERGVTLNVIAQPQKVVVVEEMIQSIRRQMGMRV
                     IAKKRALVESLRVLRRGETLAMLIDQHNRRDGLPILFFGRSAWTVASPAVLSLRTGCP
                     VFPAFLVRKTDGSFRGHLDVPIHPEPTGNFDSDVLRLTQQYTTIVENYVRMYPEQWMW
                     VHRRWRPESGTDSPRRANRKTRAIK"
     gene            complement(4575..5729)
                     /locus_tag="PKH07_00670"
     CDS             complement(4575..5729)
                     /locus_tag="PKH07_00670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33493.1"
                     /translation="MMNCLMKYGFPPPWYSLFELGSCAAVLLVLLPAAIAFCEEKEMM
                     KDSVLVGVHFFAGWWEPLPNKWIVAGSDWRENYPSRVPLLGQYNTQETMDKEIQAAAA
                     HGVDFFQILWYYNDADSEREPNSQLLNRGLEDFLQSPHSDRLKFLIEFCNHPPYQVET
                     DEEWSNCLKIWVAAMRHPSYLRVGNRLVFKVHGGHYFIRQNGADLERCRTRLQFLRNA
                     VQEAGLGEMLIGCGVGAHEQVSKDSRFAELFDFTNTYMDVPNCELTAEPHPYTVLANH
                     INSGREKHRNDVIPYVPFVAAGWYPRPWRDPRPDFEFPTDTEWRQELSRMRSDLEAHR
                     VFGLPLSDNSSQKVFTIYAWNEFGEGGFVAPTVGEKYQRLEAIRDVFGYQ"
     gene            6102..>7097
                     /locus_tag="PKH07_00675"
     CDS             6102..>7097
                     /locus_tag="PKH07_00675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33494.1"
                     /translation="MKRLPSGILIFLFALSLYVFTMGGRIYSPDCEIMFRTTESIVER
                     FETSIEPLMGFATRQSADGREYAQYGIAQSVLAVPFYLAGKALSAVVDESFLQTLRED
                     TIQYYPLSLSAYTKRFGVSFYNLFVTALTVTLVYFLTRGIYRCEKMGIFCALLYGIGT
                     VAWPQSKPFFSEATAAFFLLLCFAVQCGDCLKGQHFLLAGFALGMALLTRVDSVVAFP
                     ALGLFALLRAFETGMRRGADTRTALLRIVAFALPVILALVFIAGYNHFRFGSFTATGY
                     EDQEEGIAFETPILVGLYGQLFSAGKGLFVYSPVLILLFWALPRFVRKFPLQSLIL"
BASE COUNT         1588 a         1959 c         1869 g         1681 t
ORIGIN      
        1 ccactgataa gtcccgattt gtcaaaagca acatctgccc aattgccttc gcgggatgcg
       61 ttgatgatgc gaaggattac gcccagactg atcatcagca caccgaacac aatggcgatc
      121 agcgccagcg tttctatttc ttccatcgga tgcagtacca tggccgggat gacatgctcc
      181 agtccaaaaa cactgccgta aaacatacca aaaagaatcg aactagcacc cgccatcaac
      241 aacacaaacc ccaagttcct ggtcgcattg cggcgagaca aaaaccagcc acccaatgcc
      301 aataccagcc cgtgtccaac atcgccaaac atcatgccga acatgaagag aaaggtgacc
      361 gcaaggaacg gagtcggatt gacggagcga taggtgggaa tgccaaatcc ggttgtcaat
      421 ggttcgaaag gacgcatcca ccccgggttt ctgaatagtg cggggacatc ggctcttcgg
      481 gtgcggactc cttcaacctg gtctgccgaa accaactccg taaaggctct gccctgggta
      541 gactgctgga tttcacgaat gagtgacctt cctttgcccg tcggaaccca gccacagaga
      601 aaacgcgcgt gtcgagttgc ccgaacaaaa gaccgtgcct tggccgccat ctctccccgt
      661 cgcgcctggt aaaagatctg tatggcacgt tcggtgtttt ccgcagccca tttctcgagg
      721 tcagtccttt gtgaggccag ttcctgctca atctcggtac gcttatcctg aagcttctcc
      781 aaactgattt cgtgttgtgg aaccagtctc gagggatctt ccgcctcaaa tcctgccgcc
      841 tgtaaggctt tgtccagagc cacgcggtca cgtcggagta ccatggccag aatagtcacg
      901 cgctcgtcct ctaccgacaa gggcattacc accgacggga cgccttcgag tcgagactgg
      961 agcgtggcca agttggcttc tcgtcccgac gcaaaggaaa ccttcaggaa cgaatgcgga
     1021 cctgcgaaag cctcggcaat ctctcccgcg cgtggtgaat gaagctgccg ggaaagcaaa
     1081 tccagttggc tcttctccgt gttgagagat tccaaacgcg cctggatctt tctgactggg
     1141 gcggagattt cgttcaggga tttctgaagt tcatcgggat tcttcaaaga ctcgggaggc
     1201 agatgtgtgg gtagaggaat cagttttccc atatcctgaa gcagtgagcg tgcctttgca
     1261 gatatggcac catactgacg ctcaacaacg gcgatgtctt ctccgtggag ttcctttgtc
     1321 catgtgtgca tcagctgggg ctcaatcata tggagaacgc cagactcgga ggccagtttg
     1381 atgatctgtg cttcgtcctg ttgcaggaac acgacgctca gttcagacat gggttcggca
     1441 agaaacatgg ccttgaaaat gcctttctct cagcgaacat tcactggaaa tccgccgatc
     1501 tcatcgggtg ggatttccat cgcgatgctc tccaacagtc ggatgaggtt ctggacttcc
     1561 aggcgtttga gaaaacagta cgccaaagga gtcccaattg tgaagggaaa accagccagc
     1621 gacagtcgaa cctgtctcaa gaggatctcc cagagtatcc cttccagaag tgcggcgctc
     1681 tcgcgcggcg cacgagccag ccaacccgac agcagttcct gatacggacc caaggcaagc
     1741 tgctccaaga catctgtcac gtcggacttc tccagtgcct ttcgggtcac gctcggctgg
     1801 atacgccagc cgtggggaat caagtcttcc agcaattccg ccgtggggat ccgatagtac
     1861 atgatgcctc ggagaatcca ctgaagattc ttcaagtcca cttcgattcc caccaggcgt
     1921 gaagccaccc gtctgtcgcg ttccgacaaa gtttccagcg atgaccaaag gcggcggtag
     1981 taactctggt cgagagcgat ctccaacaca aagacactct ttcggctctg gaagagcgga
     2041 ctcgccgaca agagagtgga gccgtaggga gtacctttca gcagcagcgc gacctgttcc
     2101 agagactccg cctgaaggat ctcttcgagt ggcagatcgc cgacgccaag aggcatgaag
     2161 tcggatggcg caccggcgcg gctgcgaacc caggctctga gagcgccttt caagtcttcc
     2221 agttcgtacc aactctccag tatctcgaac acctgctcga cggaaccttt tgtcaaaccg
     2281 gcgatctcac ggtaggcggc cgcttcatca agcaagagca gtctctccag ttcagaaagt
     2341 ctgggcgttt cggagagctg agtcacaaga taaccgtagg gagtatcctt gagaacgacg
     2401 agagcgtgat ccaagtctgc acaattggcg agcgcccgaa tctgttcctc atccagcagt
     2461 cgggacatca tgcctcgtac cttggcattg agaaaggcgt attgggacag ccctcccaac
     2521 attattttgt tattcctccg tcgttgtgaa cgtcgtcagc gattggatga gattctccac
     2581 cacttcaggc agtttttcag tcagcgctct ttccatttca gagagctcac gttgggcatt
     2641 gccagcttcg gtctcggcct gctcttgtgc caagaggctt tgctgcacca caagctcacg
     2701 agctgcctgc tgtgcatgtt tctccccaga ggctatctgt tctttggctt cgcgttccgc
     2761 ctcagacact atctgtctgg cctcgtccgt tgcttgtcgc agtgaatcgg acgctgcctt
     2821 ctcctttttc aggatatcct cgatcagttg ccgcgctctc tcgaccactg ctttagacct
     2881 ctttctttga gcggatatct gtcaaacaaa agcaatctag cacctttgct cttgtcattc
     2941 cagagcaggg gatcaccttc tgaatcgccc ttcgagaatc acccgaatca ccctgagtgc
     3001 acccgaacgg tgactgatcc tgttcttgac tgatgcgggt agttcaccaa aggtcttccc
     3061 gtattccgga aggtagaaaa tcgggtcgta cccgaatcct tcatggccgc gccgattctc
     3121 ggaaatgagt ccgcaaactt caccaaaagc ctgaccgatt tccttatcgc tctcgtacaa
     3181 taccgcctgg cagacaaagc gggcagtccg ctttgccaga ggaactccct gcagttcaag
     3241 gaggactctc tcgatgtttt cggcatctgt tgaaccttcc ccgcgagcat atcgggcgga
     3301 atagactcct ggcctgccat ccaaagcgtc cacttcgagg ccggaatcct cagcgagggt
     3361 gagaagtccg gtatgtcttg cgagcgtctg tgccttcttt cgggcgttgt ccaagaaggt
     3421 agagcgatct tctacaacct cgggagcgtc tgggaactcc tccagagtga cgattcgaca
     3481 aggaagcccc ttaagtattg tttcgagttc gcggcgttta ccttgatttc ttgtcgcgag
     3541 aagaagggag cattcagtgc tcatcatttg atcgccctgg tctttctgtt ggctcttctg
     3601 ggcgagtcgg ttccgctctc aggacgccaa cggcggtgta cccacatcca ttgctccggg
     3661 tacatgcgaa catagttctc aacgatggtc gtgtactgct gtgtcagtcg cagaacatcg
     3721 ctgtcaaagt ttcccgttgg ttccgggtgg atcggaacat caagatgtcc tcggaatgaa
     3781 ccgtccgtct tgcgcaccag aaatgctgga aaaacggggc aacctgttct gagcgacaac
     3841 acggcaggtg aggccaccgt ccaggcagac cgaccaaaga acaagatggg caggccatct
     3901 cttcggttgt gttgatcaat aagcatcgcc aatgtctcgc cgcgccgcaa gacccgcagg
     3961 gattccacga gagcgcgttt cttggcaatc acacgcatgc ccatctgcct tcggatagac
     4021 tggatcatct cttccaccac gacgaccttc tgcggctgag caatgacgtt gagtgtgacc
     4081 cctcgttctg ccagaatcgc tccaccgact tcccagtttc caaggtgcgc caccagaagg
     4141 atcgctccct gtggggtatt ccggagagca ggaagattct cttcgccgat aacttcgacg
     4201 agctgggaaa gtcttcctct gaatcgcggc agagagacaa actcgaaggc agtcatggca
     4261 agactggcga gagactgctt gacgatgcgc tgtcgctgtt catgagagag ttctggaaag
     4321 gcggcctcga cgttttccaa ggcgatacgg cgtcttctgg cagcaaagag gaatcccaaa
     4381 caccccagca attgggcaaa cgcccactgt acccgacgag gcagtcggga gagtatcgca
     4441 accgcaaggc gaaaaaagaa cctgatcatg aacactcctg acacttgatc ctaccgtcgg
     4501 acgagaatac atcaggaaga agaaacagaa caactgagga gggattcttc ccgtcttcga
     4561 agcgcgtcgg atgactattg atatccaaaa acatctcgga tggcttccag tctctgatac
     4621 ttctcaccga cagtgggagc gacaaagcct ccttcgccga actcattcca ggcgtagatc
     4681 gtaaagactt tctgggaact gttgtcggat agaggaaggc cgaagactct gtgtgcctcc
     4741 agatccgatc tcatccgact cagttcctga cgccattcgg tgtcggtggg gaactcaaaa
     4801 tccgggcgtg ggtcgcgcca gggtctcgga taccagcctg ccgccacgaa cggcacgtag
     4861 gggatcacgt cgttcctgtg cttttctcgt cccgagttta tgtggttagc aagaacggtg
     4921 tacggatgag gttcagccgt caactcacag ttgggaacat ccatataagt attagtgaag
     4981 tcgaaaagtt ccgcgaagcg cgagtctttg gaaacctgtt catgggcacc gaccccacat
     5041 ccgatgagca tctctcccaa gcctgcctcc tgaactgcat tccgaagaaa ctgaagacgt
     5101 gtccgacatc tttcgagatc ggcgccgttt tgtctgatga aatagtggcc accgtgtact
     5161 ttgaacacta atctgttgcc aacgcgcagg tagctcggat gacgcatcgc tgcaacccag
     5221 atcttgagac agtttgacca ctcttcatcg gtttcgacct gatagggtgg gtgattgcag
     5281 aattcgatga ggaacttcag cctgtctgaa tgcggagatt gcaggaaatc ctccagcccg
     5341 cgattcagca actgagagtt cggctccctc tccgaatcag cgtcattgta gtaccagagt
     5401 atctggaaga aatccacgcc gtgtgctgcg gcggcttgaa tctctttgtc catcgtttcc
     5461 tgtgtgttgt actgccccaa taggggaact cgggatggat agttctctcg ccagtcactg
     5521 cccgcaacaa tccatttatt gggaagtggt tcccaccatc ctgcgaaaaa atgcacacca
     5581 acaagaaccg agtccttcat catctccttc tcttcacaaa aggcaatggc tgccggcaga
     5641 agcaccagta ggactgccgc gcatgatccc agttcaaaca ggctgtacca tggcggagga
     5701 aatccatatt tcatcaggca gttcatcatc ctctcccagt gacacagagt ccgagagtag
     5761 cgcgtcgttt attgagcaag cagcccacgc gcctgcaggt gctcacctgc tcggaaaaag
     5821 gtcaactgag actacatcaa gcaggccacg cctgcaattg ctcatcagtt gaaaagaggg
     5881 tatccgaata ccgtatcctg tgggtttcag ctgtgtcggg cgaagaacca tgcacgaacg
     5941 aacgatttcc gttgaaggcg ttgctgagaa aatctatgct tgcattctca gacgccaagg
     6001 tcaatgcggt gcctgaggct gtttgaaaga tgccgatgga tactgaggtg gcaggtgccg
     6061 cgcgacccag aggccgcaag cgacagggag atgtacgatc cttgaaacgg ttaccttccg
     6121 gaatactgat ctttctcttt gccctttcgc tctacgtttt cacgatgggg gggcgcatct
     6181 attcgccgga ttgtgaaatc atgtttcgta ctacggaatc catcgtggag cgtttcgaga
     6241 cttcgatcga acctctgatg ggattcgcta cccgccagag cgccgacgga agagagtatg
     6301 cccaatatgg aattgcccag tcggtgcttg cggtgccttt ctatctggca ggcaaggctc
     6361 tgtctgccgt ggttgatgag tcctttctgc agacgctgcg tgaggatacg attcagtact
     6421 acccgctgag cctttccgcc tatacaaagc gatttggcgt ttccttctac aatctctttg
     6481 tcaccgcttt gactgtgacg cttgtttact ttctgactcg tggaatctac cggtgtgaga
     6541 agatgggaat cttctgcgcc ttgctctatg gtattggaac cgtggcgtgg ccacagtcaa
     6601 aacctttctt cagcgaagcg actgcggcat tcttccttct gctgtgtttt gccgtccagt
     6661 gcggagactg tctgaaagga cagcattttc tgttggcagg ttttgcgctg ggaatggcgc
     6721 ttttgacgcg agtggactcc gttgtggctt ttcccgcgct gggactgttc gcgctcttgc
     6781 gtgcatttga gacaggcatg cgaagagggg cggacacaag gactgcactg ctgcgcattg
     6841 tcgcctttgc acttcccgtg attcttgctc tggtcttcat cgctggctat aaccattttc
     6901 gttttggatc cttcactgcg acaggatatg aggatcaaga agaagggatt gcttttgaga
     6961 cgccaatcct ggtcggtctg tatgggcagt tgttcagtgc gggcaagggt ttgtttgtct
     7021 attcgcctgt cctgattctg cttttttggg cgttgccgcg atttgtgaga aagttccccc
     7081 tgcagagtct gattctg
//
LOCUS       DAOQUC010000020         6783 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_12360_length_6783_cov_3.437574, whole genome shotgun sequence.
ACCESSION   DAOQUC010000020 DAOQUC010000000
VERSION     DAOQUC010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6783
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12360_length_6783_cov_3.437574"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..716
                     /locus_tag="PKH07_00680"
     CDS             <1..716
                     /locus_tag="PKH07_00680"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HNT33495.1"
                     /translation="FSVAYPPNPDPAKRPVWLFFDQRFAHSYILDGSRVPFDSGRVVD
                     YLAARGDVHPHKIGWMGSSSTGIPGLAVATQGTRRLAAVVAFVSTGAYRQWFETWHTN
                     GLWKGETPELWPETQQLLDKYDSILHVSKMYPTAILMVSGGADTVVDPKTAQAFVEAA
                     RPYYTSEPDRLRLVIYDGFGHNLPIDIVQMYIEHWFHRYMHPTEEPPQPAVPPADLQE
                     SVTRTQINATDHKAIIGAH"
     gene            775..1338
                     /locus_tag="PKH07_00685"
     CDS             775..1338
                     /locus_tag="PKH07_00685"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNT33496.1"
                     /translation="METTGLRMILKNLEGVPQHPLPEGFSLRFYKPADEQTWVNIHKA
                     ADSTIEYSVDLFRKEFPGESALLEERQYYLCDKIGTPIGTATAWFHSDYFGLDYGLVH
                     WVAIVPEYQGKGLSKPLMSAVCMRLKELGHSRARLATSTARLPAVNLYLKLGFVPDIQ
                     NEKDVTAWRFVMSQLHRDRLECLMLGQ"
     gene            1380..3005
                     /locus_tag="PKH07_00690"
     CDS             1380..3005
                     /locus_tag="PKH07_00690"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HNT33497.1"
                     /translation="MIIDVAFCGVKSVALVLLFVVFPTTAMAKDVRHYRNPLDVFVAD
                     PQILKDGDTFYLYGTTDSPNGIEVHTSKDLVHWRRYGHCYKKTKTSWGQDCFWAPEVI
                     KAGETYYLFYTAFSPVHGVRNICVATSDSALGPFLDVKTPVLPVERGFIDGHPLFDPV
                     SGRHYLYALEENLRPPQLVVARLSEDLTRIDSEITLCFEPSQPWEYGWVEGAFVIYHK
                     SAYYMTYSGCGYQSPNYAVGYATAPSPTGPWTKYEGNPVLAKSAAVSGPGHNSLVLSP
                     DGRELFIVYHRHLTFFGGWQRELAIDRVLFRPNRGCLDTLTIPGAPTHDLQPLPSGSR
                     PHRVGCSDDFEGLDLDWSRWTVFGEDSQKWRLENGCLCIETQNGDVYRERVDGRNIFL
                     QYAPPGDFCVSTKVEFEPEKNVEQAILTIWQDQSNYLKLACVMVDSKCFEAAREIGGH
                     FDSVLVPNPLGSTFHMQIRKKGTHYTYSYSADGVEWTEIGSGFDATLQNIKFGIGAMS
                     PPEGATRLARFHQLEIESSKQSGKKSLLPWKRN"
     gene            3017..4306
                     /locus_tag="PKH07_00695"
     CDS             3017..4306
                     /locus_tag="PKH07_00695"
                     /inference="COORDINATES: protein motif:HMM:NF024120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HNT33498.1"
                     /translation="MAYFLGILMVFFFASGSLATETNPKDLEGVISVRAHGVVADGTT
                     DDTAALQSALDEAGKTGGRVYLPPAQYLIKGSLKVPEGVTVMGVACSPHYSTPLIGTV
                     ILATGGRDDENASALFEMPGSTMVTGLTIYYPEQKAADIRPYPWTFHMYGHDTTIENV
                     TLVNSYNGIRVGPEGNVRHRIRSVYGCVLRRGLLVDACTDIGRVDNVQFHGHWWWMKE
                     LGGDYELVNNYMIQNLEAFIFGRTDWEYVTNTFVFPVNVGYRFIQTPSGSCNGHFCGI
                     GADYAQRCIVVDQIQHMGLLITNGQFVAFAGENPIEIEINETCTGQIRLVNCAFWGPS
                     VQNVVSRGQSFLSLSDCFLSSDRAEVGPLVEVASGKIQIRGCSFAGAKSKCIALRKGV
                     RHAIVTENNAVEGVSIINEIGRKAIIRNNEPPASKDK"
     gene            4515..6152
                     /locus_tag="PKH07_00700"
     CDS             4515..6152
                     /locus_tag="PKH07_00700"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HNT33499.1"
                     /translation="MQIEKSTMTQRACNNTKNRVPDSLMNLAQGRTDLAWYQAETGHI
                     LVLGTLDPGLLDYVYRFAPEIRSDIELLRRSENLVLDIKTCSAWRGGESVQKIEPLEC
                     VELIANLSVIASFPKKCLSADDLAAPYVSVILGAGKGSRMANDDSQKVCTYIGTRSAT
                     ERALEIYRRTGFTRNILVVGHGSVKVVKHISEVCPDTTFVLQARLLGTGHAAQQAMYL
                     LEEQGFKGDVLVVAGDKVIDPAALQLLRQEFEASGADLMALCTAKSRWPNSGRILLNP
                     EGTIEAIIETMDIKKMKIVSSLLEQTESGGQLDPQTVRDWIWKEVPNEKKRRKAFPHA
                     FLDRLQKVEPLDREELASFINPTDAFFEYRTTDGNVVRLTGDQIEQSARVVNVSVYLF
                     KSDAFYFAVKRITNNNAQMEYYLTDIFEILCKARCEKGMRQFKVLPLELEDTEQVLGF
                     NTEQELKDILIYLRSKMVAQLRQRGVLVDETAEKSDVFWVDDLDSIEKIGSGTRILGD
                     AVVDLGPDPNHRIGANCVIDNARVYRETIPDGTVRRG"
     gene            6202..>6783
                     /locus_tag="PKH07_00705"
     CDS             6202..>6783
                     /locus_tag="PKH07_00705"
                     /inference="COORDINATES: protein motif:HMM:NF016817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HNT33500.1"
                     /translation="MSFSLTRLSSGTVLRHLFRVPGTSLSLPVYFLSGTSPGATLLVH
                     AALHGSEVVGVEVIHRLLETLAPRDISGRVILVPVVNIPAFEEGRRCFSQTNQDLNRV
                     FPGRSNGTLTERIAHLYFHKLVRCSDAIVDLHSTESPVLMLPHVRQRVLQPTQAHRDL
                     MRATGLSALWIGPGVLGMLQVQAAGIGKPCVTVE"
BASE COUNT         1534 a         1687 c         1900 g         1662 t
ORIGIN      
        1 cgttctccgt tgcctatcct cccaatccgg atccagccaa gcgaccggtc tggttgttct
       61 ttgatcagcg tttcgcccac agctacatcc tggacggttc ccgcgttcct ttcgatagtg
      121 gccgtgttgt ggattatctt gcagcgcggg gtgatgtcca tcctcataaa atcggctgga
      181 tgggcagttc ctcaaccggg attccaggcc ttgctgtcgc cacgcaaggc acgcgaagac
      241 tggcagccgt ggttgccttt gtcagcacgg gagcctatcg gcagtggttc gagacgtggc
      301 ataccaatgg gctctggaag ggtgagacac cggaactttg gcctgagaca cagcagctgc
      361 tggacaagta tgattccatc ttgcacgtga gcaaaatgta ccctaccgct attttgatgg
      421 tcagtggagg agccgatacg gttgtggatc cgaaaacagc acaggccttc gtcgaggcgg
      481 cacggcctta ctacacctcc gaacccgacc ggcttcggtt ggtcatctat gacggttttg
      541 ggcataacct gcccatagac attgtgcaga tgtatatcga gcattggttt caccgataca
      601 tgcacccaac agaggaaccg ccacaaccgg cagttccacc cgctgatctt caggagagtg
      661 tgacgcgaac gcagattaac gcgacggatc acaaggctat catcggcgcc cattgatgca
      721 ggacggtcag gcgtacggac tcgtgatcct gaaggggaaa gaagagtatc tacgatggag
      781 accacaggac tgcggatgat tctcaagaat cttgagggcg ttcctcaaca cccattgccc
      841 gagggcttct ctttgagatt ctacaaacca gcagatgaac agacatgggt aaacattcac
      901 aaggcagcag acagcacaat cgagtactcc gtggatctat tcaggaagga gtttcctgga
      961 gagagcgcac tgctggagga aagacagtac tatctttgtg acaaaatcgg gacaccgata
     1021 ggcactgcca cagcatggtt ccattcagac tatttcggct tggactacgg cttggtgcat
     1081 tgggtagcca ttgttcctga gtatcagggc aaagggcttt caaagccttt aatgagcgct
     1141 gtttgcatga ggttgaagga gctcggacac agtcgagcca ggttggcgac ctcaactgcg
     1201 cggctgcctg cggtgaatct ctatctgaaa cttggattcg tgccggatat acagaatgag
     1261 aaagatgtga ctgcgtggcg gtttgtgatg agccagctgc atcgagatcg tcttgaatgt
     1321 cttatgctgg gtcagtagcg ggttccagat gtttgaacac ttttacagga gcagttgaga
     1381 tgatcattga tgtggcgttt tgcggcgtga aatctgtggc gttggtgttg ttgttcgttg
     1441 tgtttcccac aactgcgatg gcaaaagacg ttcggcacta tcggaatccg ctggacgtct
     1501 ttgttgcgga tcctcagata ctcaaggatg gggacacttt ctatctctat ggcactacgg
     1561 actctcctaa cggtattgag gttcacacat ccaaggatct ggttcattgg cgacgttacg
     1621 gccactgcta caaaaagacc aagacttcct ggggacagga ctgtttctgg gcacctgaag
     1681 tgatcaaggc tggcgaaacc tattatctct tctataccgc gttcagtccc gttcatggag
     1741 tcagaaacat ctgtgtggcc acgagcgatt cggctttggg accctttctc gatgtcaaga
     1801 ctccggtgtt gcctgtcgag agaggattca ttgacggcca tccgttgttc gatccggtca
     1861 gtggacgaca ttatctctac gctcttgagg agaatcttcg tcccccacag ctggttgtgg
     1921 cacgcctttc ggaagatctg accaggattg attctgagat cacgctctgc ttcgaaccct
     1981 cgcagccttg ggagtacggt tgggtggaag gcgcgtttgt tatctatcac aaatccgctt
     2041 actacatgac ctactcaggc tgtggctacc agtctcccaa ctacgctgtg ggttacgcca
     2101 ctgcgccaag tccgacggga ccgtggacaa agtatgaggg caatcccgtg cttgccaagt
     2161 cagctgctgt ttccggaccg ggacacaact ccctggtact ttctcccgat ggtcgggaac
     2221 tgttcatcgt ttaccatcgc catctgactt tctttggtgg atggcagcgg gaactggcca
     2281 ttgatcgagt cctgtttcga ccgaatcgag gttgtttgga tactctcaca attcccggag
     2341 cgcccaccca tgatctgcag cccttgcctt cagggagccg acctcatcgg gttggctgct
     2401 cggatgattt tgagggtttg gatctggact ggagtcgttg gacggttttt ggtgaagaca
     2461 gccagaagtg gcgactcgaa aacggttgtc tctgtatcga gacccagaat ggcgacgtct
     2521 atcgggaacg tgtggatggg cgcaatatct ttctccagta tgcgcccccg ggggatttct
     2581 gtgtcagcac gaaagtggag tttgaacctg agaagaatgt ggagcaagcc atcctcacca
     2641 tctggcagga tcagagcaac tatctcaaac tggcgtgtgt gatggtagac tcaaagtgtt
     2701 ttgaagccgc gcgagagatt ggtggtcatt ttgactccgt gttggttcct aacccgttgg
     2761 gaagcacatt tcatatgcaa atacggaaga aagggacaca ctacacctac tcctacagtg
     2821 cggatggcgt ggaatggacg gagattggtt ccggtttcga cgcaaccctt cagaatatca
     2881 agttcggcat tggtgcgatg tctccgcccg agggcgcaac tcgacttgca agatttcacc
     2941 aactggaaat cgagtcatcg aagcaatcgg gcaaaaagtc gcttcttcct tggaaacgca
     3001 attgaggagg tgtgatatgg cttacttttt gggtatcctg atggttttct ttttcgcgtc
     3061 tggttcactc gcaacggaga ccaaccccaa ggatctcgag ggtgtcatct ccgtcagagc
     3121 gcatggagtg gttgccgatg gtaccaccga tgacactgct gctctgcaga gtgctctgga
     3181 cgaggctggc aagacgggtg gccgggtgta tcttcctccg gcgcaatatc tcatcaaagg
     3241 aagcctgaaa gttcccgagg gagtgactgt catgggggtg gcctgctccc cgcactatag
     3301 caccccgctt atcggtactg tcatccttgc cacgggggga cgggatgacg aaaacgcttc
     3361 ggcactcttc gaaatgcctg gctccaccat ggtgacaggt ctgaccatct actacccaga
     3421 gcagaaggca gcggatattc gtccctatcc ttggactttt cacatgtatg gccatgatac
     3481 aactatcgag aatgtcactc tggtcaatag ctacaatgga atcagggtgg ggcccgaggg
     3541 aaacgtccgc caccgaatca ggagcgtcta cggatgtgtc ttgcgccgag gcctgcttgt
     3601 ggatgcctgt accgacatcg gtcgcgtgga taacgttcaa tttcatgggc attggtggtg
     3661 gatgaaggaa ctggggggcg actatgaact tgtgaacaac tatatgatcc agaatctgga
     3721 agcattcatc tttggccgca cagactggga gtatgtgacc aacacctttg tttttcctgt
     3781 caacgttggc tatcgcttta ttcagacccc gtcaggttcg tgcaatggtc atttctgcgg
     3841 aatcggagcg gattacgcgc agcgctgcat tgtggtggac cagattcagc acatgggctt
     3901 gctcatcaca aatggtcagt ttgtcgcttt tgccggtgag aatcccatcg agattgagat
     3961 taatgagacg tgtactggac agattcggtt ggtcaactgt gcattttggg gcccatcggt
     4021 acagaatgtt gtctccagag gacagagttt tctttccctt tccgactgct ttctgagcag
     4081 tgatcgtgca gaagtcggcc cactcgttga agtggcaagt ggcaagattc agattcgtgg
     4141 gtgcagtttt gcgggtgcca aatccaagtg tattgctttg agaaagggag tcaggcatgc
     4201 catcgtcaca gagaacaacg cagtagaggg tgtcagcatc atcaacgaga tcggtcgaaa
     4261 agccatcatc cgaaacaatg agccaccagc cagcaaggac aagtagagcc gtctcactca
     4321 atggatttga gctctgagca agcatatccg tcacagatgg tctgaacttg tccctttgtg
     4381 tctttcgctc acgctgaacg acgacggtgc cggagagcac tgacttatct ccgatgtggc
     4441 cttgttgcgc acactgaaaa ctgctacatt cgcccagatt tgccccacca tccaggtctt
     4501 ggtggaattc ttcagtgcag attgaaaaga gtacgatgac gcaaagagcc tgcaataaca
     4561 caaagaaccg agtaccggat tcattgatga atctggcaca aggccggacg gatctggctt
     4621 ggtatcaggc cgaaaccgga cacattctgg ttctgggaac attggatcct ggactgcttg
     4681 actatgtcta ccgattcgct cctgagattc gatcggacat tgagctgttg agacggagcg
     4741 agaacctcgt tctcgacatc aagacatgtt ccgcgtggcg tgggggggag agcgttcaga
     4801 agatcgagcc cctcgaatgt gttgaactga tagcgaatct cagcgtcatc gcgtcgtttc
     4861 ccaaaaagtg cctttcggca gatgatcttg cggcacccta cgtatccgtg attctgggag
     4921 ctgggaaggg ttcccgcatg gctaacgatg acagccagaa agtatgcact tacatcggca
     4981 cccgaagtgc gacggaacgg gcattggaga tctatcgccg aactggattc acccgaaaca
     5041 ttctggttgt tggtcacgga agcgtcaagg tcgtcaagca tatctccgaa gtatgccccg
     5101 atacgacctt cgttctgcaa gcgagacttc tgggaacagg acacgctgcc cagcaggcga
     5161 tgtatcttct cgaagagcag ggcttcaagg gtgatgtcct cgttgtggcc ggtgataagg
     5221 ttattgatcc tgccgcactg cagctgctgc ggcaggaatt tgaggcaagt ggcgcggact
     5281 tgatggctct ctgtactgcg aagagtcgct ggccgaactc aggtcgaatt ctcctgaacc
     5341 ctgaaggaac gatcgaggca attattgaga cgatggacat caagaagatg aagatcgtgt
     5401 cgtctctcct tgagcaaaca gagtctggtg gccaacttga tccgcagaca gttcgagact
     5461 ggatttggaa agaagttccc aatgagaaga aaagaagaaa ggctttcccg cacgcttttc
     5521 tagaccgatt gcagaaggtt gagcccctcg atcgagagga gttggcatcg ttcatcaatc
     5581 cgacagacgc cttctttgaa taccgaacca ctgacgggaa cgtcgttcgc ctgacgggcg
     5641 atcagatcga acagtccgcg cgtgtggtca atgtttccgt ctatcttttc aagtcggatg
     5701 cgttctattt tgccgtcaag aggatcacca acaacaacgc tcagatggag tactatctga
     5761 cggacatttt tgagattctc tgcaaagccc gatgcgaaaa ggggatgagg cagttcaagg
     5821 tgcttccctt ggaacttgag gacacggaac aggttctcgg tttcaacacg gagcaggaac
     5881 tcaaggatat tctgatctac cttcgaagca agatggtggc acagcttcgg cagcgagggg
     5941 ttcttgttga tgaaactgct gaaaagtctg atgtgttctg ggtggatgac cttgattcca
     6001 ttgaaaagat cggttcggga acacgcattc tgggcgatgc ggtagttgat ctcggtccgg
     6061 atccaaatca tcgcatcggt gccaactgtg tcatcgacaa tgcgcgggtc tatcgcgaga
     6121 cgattccgga cggaaccgtc cgccgaggtt agagagcacc caggttcccg gtctcaaaga
     6181 tgcttcctgg gagagagatg gatgagtttc tcgcttacca gactttcatc gggtacggtg
     6241 ttgcgccatc tctttcgcgt tccggggact tccctgtcac tccctgtcta ctttctctcc
     6301 ggaaccagtc ccggtgccac tttgttggtc catgcagcgc tccatggcag tgaggtcgtt
     6361 ggcgttgaag tcatccatcg gctgctcgag acactcgcgc cacgagacat cagtggcaga
     6421 gtcatccttg ttcccgttgt caacattcca gcctttgagg aaggcaggcg atgcttttcc
     6481 caaaccaatc aagacttgaa cagagtcttt ccagggcgct ccaacggaac gttgaccgag
     6541 cgcatcgcgc atttgtattt tcacaagctt gttcggtgca gtgatgccat tgttgatctt
     6601 cattcgactg agtcgcccgt cttgatgctt ccacatgtgc gacagcgtgt gttgcaaccg
     6661 acacaagccc atcgggatct catgcgagcg acagggctgt ccgcgctatg gattggccct
     6721 ggagtccttg ggatgcttca ggtgcaagca gcgggtattg gcaaaccgtg tgtgacggtt
     6781 gag
//
LOCUS       DAOQUC010000021         6749 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_12452_length_6749_cov_3.262175, whole genome shotgun sequence.
ACCESSION   DAOQUC010000021 DAOQUC010000000
VERSION     DAOQUC010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6749
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12452_length_6749_cov_3.262175"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1533
                     /locus_tag="PKH07_00710"
     CDS             <1..1533
                     /locus_tag="PKH07_00710"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HNT33501.1"
                     /translation="GVVQDELETSFGLRWYRFDPNKGFFLNAQNLKLQGTNRHQDFPG
                     LAYAVPNFIHRLDIQNMKDMGVNFWRTSHYPHDPETLDMCDRLGILVLEEIPVMKEIG
                     HPENYTRNAQQMACEMIRAHRNHPSIVLWGIAGEIDAPEEISSRVTKAILETFRQEDP
                     TRLTTMHCPRSESIETFVDVIGTDLKRSDDDHQRHPQSCYMVSEFSVSFSGRGLHDGE
                     PYNEEDSNIAHENHLRGINERRWLAGCAIWNQFDWIGETYDMVFPHVTAFGMCDVWRI
                     PKDVYFFYKSQWNPEPMVHICGHWTWPGKENRIRTVKVYSNADRVEMLLNGVSLGVME
                     MPCEPHLAHPPFIWQVPYTSGVLTAKALFHNGTELVDQRKTAGRPYAVRLTASPEELV
                     AGDLDSHVLITAEIVDREGTVVPDAYLPVSFSNYGCGKLLPQTWLEYGTGCTWRAVAG
                     VTRILLRATEFGGRAVITASSAGLVMGRLVVNAVDSRGWRNPFKFWRDPQTEVEEPSP
                     FI"
     gene            1611..3893
                     /locus_tag="PKH07_00715"
     CDS             1611..3893
                     /locus_tag="PKH07_00715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33502.1"
                     /translation="MNILPTDPWRSGTTEEIERYFEDLRRWNFDIPYSAFYAFPHETL
                     IGEAKEKYKAFADRARAERLPACVQIQSTVGYLDTPDLIEAGQYHLDNTPIVYEHYHG
                     KGAKNFFGSFASERWFEYVMNVARTLREYGYDWVVFEEPMYRVDIPGTKDAFYKLFRE
                     RYPELEYPTRRDESLSYLTLQALKMDVLVDFYRRCCLEAKRMGYSKVGIMPWFFTPTY
                     ENTPMESWATCCDLGRLTFLDDLDFIVVRMQPDNVWAEAMVSSGGESLPRLAYLENLS
                     QALGKPTIAVNNPTNEHVSHSQEGTKDLLDYGYFARYTLAALAAAPSGMSRHWYGKNY
                     DQDERHMDLYTECNPFFGRFGGPVSPFAMVFSHRGTVRVMPRPWLETWRSFSTLLQGV
                     LFEHRLPCLTFFADSLQSCIERHPEVQTLILTPYFPISRSETALLESWLSDKPGRSVV
                     YVGSDYGYTYDFETSIYHWAARTPPEMISLCGIDPSSYSPLRRDDRISLDFVGKCPGD
                     AFLGESLTVKTSGTAKVAFTDERVQVLYRDRQTETPVIAKREIGNGSHVWYFGLSLDG
                     IEAGESISPACAPFPVISLLRRQTATSEFPLVVDAQPGILWNQTRSGFLVISNTTEED
                     SSVVLKKANGDYWDCRQECWVGPGCYEIGKLSFSVIRVVPYGSPFLDILNVFSIESIQ
                     EIPEKLTIKGCFRQDLCIVSTSPPEKVMLNSVPLDWDVESIEVGWGILPVFDDHCEGL
                     LEVFWSEPRIAELSSEVGNL"
     gene            3896..4627
                     /locus_tag="PKH07_00720"
     CDS             3896..4627
                     /locus_tag="PKH07_00720"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNT33503.1"
                     /translation="MEPLQWLTAILCAFLIGIAKTGVPGVAILGTTLMAAVMPAKAST
                     GFVLPLLIIGDIYAVIYYRRNAVWKHLLPLFPWAILGVLIGYLLMDKVTDAQLRPIIG
                     AIVLVVLALNQLKSLKQGDDLAVPDHWSFAAAAGLAAGTTTMMANAAGPIMAIYLLAM
                     RLPKTEFIGTGAWYFLFVNLIKVPFSYDLGLITTHSLGLNAILIPVVVIGAVLGVYLL
                     RVIPERVFKNLVVVFAAVAAIKLFF"
     gene            4829..6679
                     /locus_tag="PKH07_00725"
     CDS             4829..6679
                     /locus_tag="PKH07_00725"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HNT33504.1"
                     /translation="MAKHLILIPVPQRLVSLKGEYELPLSGSIVCLGDPSLTFPVARI
                     IQTDLLAMQRVEWSLRSGSAGGAVGSELTIRVTAKLDIPAQGYSLRVTRTGIELNAST
                     AQGAFYGSMTLRQILRQCEGALPACTISDHPDFAVRGVMLDVCRDKVPTLETLFQYVD
                     ELSELKINQLQLYTEHTFAYRNHPEVWAQASPLTGEDILRLDAYCRERFIELVPNQNS
                     FGHMHRWFDIPKYLDLAECPEGSTAPWGGHFGPFTLDPTNPNSLALLEDLFADLLPHF
                     TSRQFNVGCDETFDLGQGRSKTACDKKGKDRVYLEFLLKIHRLVRKHGRKMQFWGDII
                     LQHPDLIPELPKDVIALNWGYDANHPYSKQCPLFAKSGIPFYVCPGTSTWAAIAGLSD
                     NAVANIRNAAENGLRNGALGLLNTDWGDCGHWQYLPASYVGFAAGSALSWAFKVNRDV
                     NLVDALDMHVFKDSSRSMGRLAYDLGNTGVRTNNPVNNRHLFWCLQRPFSPEVLKVGT
                     PKQIRSAMAEIEASICSLERSRMNRSDAALIRDEFANAARMLLDACKRGLVAKQKAAS
                     RGSILELDSEMRIILGEHRRLWCARNRVGGLQDSAAILEQRLREYGELLK"
BASE COUNT         1515 a         1675 c         1832 g         1727 t
ORIGIN      
        1 ggtgttgtcc aagacgagtt ggagacctcc ttcggcttac gatggtaccg cttcgatccg
       61 aacaaaggat tcttcctgaa cgctcagaat ctcaaacttc agggaaccaa ccggcatcag
      121 gatttccctg ggcttgccta tgcagtgccc aatttcatcc atcggcttga catccagaat
      181 atgaaggaca tgggcgtgaa tttctggcga acatcccact atccccatga tcccgagact
      241 ctggatatgt gtgacagatt ggggatactg gtcttggaag agattccggt gatgaaggag
      301 attggacatc ctgaaaatta cacacgaaat gctcaacaga tggcttgtga gatgattcgg
      361 gctcatcgca accacccttc gattgtgctc tggggaatcg ccggtgaaat tgatgcccca
      421 gaagagatct cgagtcgagt caccaaggcg atcttggaga catttcgcca ggaagaccca
      481 acgaggctga caacgatgca ctgtccacgc agcgagagca ttgaaacctt tgtggatgtc
      541 atcggaacgg atttgaagcg ctctgacgac gaccatcaac gccatccgca gagttgctat
      601 atggtcagcg agttcagcgt ttccttctcg ggcagaggac tccacgatgg ggaaccttac
      661 aacgaagagg attccaatat cgctcacgag aatcatcttc gaggtatcaa tgagcgtcgt
      721 tggcttgccg gctgtgccat ctggaatcag tttgactgga ttggtgagac ctatgacatg
      781 gtctttcctc acgttactgc ttttgggatg tgcgatgtct ggcgaatacc caaggacgtc
      841 tatttcttct acaaaagcca atggaatcca gagccgatgg ttcacatctg tggccattgg
      901 acttggcctg gtaaggaaaa ccgaattcgc actgtgaagg tctatagtaa tgcggatcgt
      961 gtcgagatgc tactcaacgg tgtatccttg ggtgttatgg agatgccttg cgagccgcac
     1021 ttggcacacc cgcccttcat ctggcaagta ccctacacgt ccggtgtgct taccgccaaa
     1081 gcccttttcc acaacgggac ggagctggtt gaccagcgaa agacggctgg aaggccctat
     1141 gctgttcgcc tgactgcatc gcccgaggaa ctggtggcgg gagacttgga cagtcacgtt
     1201 ttgatcacgg cggagattgt ggacagagaa ggaactgttg ttccagatgc ctatctgcct
     1261 gtgtcattta gcaactacgg ttgcgggaaa ctgctcccgc agacatggct tgagtatggt
     1321 acgggatgta cgtggagagc agttgccgga gtgactcgga ttcttcttcg ggcaaccgaa
     1381 tttggtggca gagcagtcat tacagcctct tccgctggtc ttgtgatggg caggcttgtc
     1441 gtcaacgcag ttgattcgag agggtggcgc aatccgttca agttctggcg tgatcctcag
     1501 actgaagttg aggaacccag cccattcatc tagcatgatt ccttggattt ctctgacatc
     1561 agtgggaacc aactgtacgg gagtgaatca ctaacaatga ggtttgaccg atgaacatcc
     1621 tgccaacaga cccttggcgt tcgggaacga ctgaggaaat tgaacgctat tttgaagatc
     1681 tgcggcgctg gaatttcgac attccataca gtgcgttcta cgcttttcct catgagacgt
     1741 tgatcggcga ggcaaaggag aagtacaagg cctttgcgga tagggcaaga gcagagagac
     1801 tgcctgcctg tgtacagatt cagtccacgg tggggtacct ggacacaccc gaccttatcg
     1861 aggcggggca gtatcatctg gacaacaccc caattgtcta cgagcactac catggcaaag
     1921 gcgcaaagaa cttttttggc tcgttcgctt cagaacgctg gtttgagtac gtgatgaacg
     1981 ttgcccgaac tcttcgggag tatggttatg attgggttgt ttttgaggaa ccgatgtatc
     2041 gagtggatat ccctggaacg aaggatgcct tctacaagct ctttcgggaa cgttatcccg
     2101 agttggagta tccaacgcga cgagatgaga gtctctccta tctgacactt caggctctca
     2161 agatggatgt cttggtggac ttctacaggc gatgctgcct tgaggcgaaa cggatgggtt
     2221 attccaaggt tggtatcatg ccatggtttt tcacgccgac ctacgagaat actcctatgg
     2281 agagctgggc gacgtgttgt gatcttggaa ggctcacttt tctcgatgac ctggacttca
     2341 tcgttgttcg catgcagcct gacaatgtct gggcagaagc tatggtctct tccggcggcg
     2401 agtcattgcc aaggcttgct tatctggaga atctttccca agcgctcgga aagcctacta
     2461 tcgccgtcaa caacccaacc aatgagcacg tctcccacag tcaggaaggc accaaagacc
     2521 ttcttgacta cggctacttt gcccgataca cacttgctgc tcttgctgcg gctccgagcg
     2581 gcatgtcgcg tcactggtac ggcaagaact acgatcagga cgagcggcac atggatcttt
     2641 acactgaatg caatccgttc tttggtcggt ttggcggtcc ggtcagtcca tttgccatgg
     2701 ttttctctca tcgaggcacg gttcgggtga tgccgcgacc gtggcttgag acatggcgtt
     2761 ccttttcaac gctgttgcag ggcgttctct ttgagcacag actcccctgt cttactttct
     2821 tcgccgactc acttcagtcc tgcattgagc gccatcctga ggtgcagacg ctcattctga
     2881 ctccatactt tccgatcagc cgctctgaga ccgccctact ggaatcatgg ctctcggaca
     2941 aaccaggaag aagcgttgtc tacgtaggca gcgactacgg atacacgtac gatttcgaaa
     3001 cgtctatcta tcactgggca gcgcgaactc caccggagat gatctcactg tgtggaattg
     3061 acccttcgtc ttactctcct cttcggaggg atgatcgcat ctcactggat ttcgtcggca
     3121 aatgtcctgg tgatgcgttt ctgggcgagt ctcttaccgt caagacatcg ggaactgcga
     3181 aggttgcttt tactgatgag agagtgcagg ttctctatag agacaggcag acagagactc
     3241 ccgttattgc taagcgcgag attggcaatg gcagtcatgt ttggtatttc ggattgagtc
     3301 tggacggcat tgaggcaggt gaatccatct cacctgcttg tgccccgttt cccgtcatca
     3361 gtttgcttcg aagacaaact gcaacatctg aattcccgct cgttgttgat gcgcaaccag
     3421 gcattctttg gaatcagaca cggagcgggt tccttgtgat cagcaacacc acagaagaag
     3481 actcgtccgt ggtgttgaag aaggccaacg gggactactg ggactgccgt caggagtgtt
     3541 gggtcggtcc tgggtgctac gaaattggga aactgagttt ctctgtaatt cgagtagtgc
     3601 cctatggttc tccctttctt gacatcctga acgtcttctc tatcgaatct attcaagaaa
     3661 ttcccgaaaa acttaccatc aagggttgct tcaggcagga cttgtgcatt gtcagcacgt
     3721 ctccgccaga gaaagtgatg ctgaactcag tgccactcga ttgggatgtt gaatccatcg
     3781 aggtgggttg gggaatcctt ccagtgtttg atgaccattg tgaaggactg cttgaagtat
     3841 tctggtctga gcctcggata gcggaactct ccagcgaagt ggggaatctg tgagcatgga
     3901 accgctgcag tggcttacag caattttgtg tgctttcctt attggaattg ccaagacggg
     3961 agttcctggc gttgcgatac ttggaacaac gcttatggca gcggtcatgc ctgcaaaagc
     4021 atctacggga ttcgttctgc cacttctgat catcggagac atatacgctg tcatctatta
     4081 ccgacgcaac gctgtctgga agcatctgct tccgctgttt ccctgggcga tcttgggcgt
     4141 tctgattggg tatctgttga tggacaaggt cacagatgcg caactgcgtc ccatcatcgg
     4201 cgcaattgtc ctcgtcgtgt tggcgttgaa tcagttgaaa tctctgaaac aaggggatga
     4261 tctggcagtt ccagatcatt ggtcttttgc ggcggctgcg ggacttgcgg caggtacaac
     4321 gacaatgatg gccaatgctg caggacccat catggcaatc tatcttcttg caatgaggct
     4381 tcccaagacc gagttcattg gaacaggggc atggtacttc ctgtttgtga acctcatcaa
     4441 ggttcctttc agttatgatc tggggctgat caccacccat tcgctcggtc tgaacgccat
     4501 tctcattcca gttgtagtca ttggtgctgt gcttggcgtc tacctgctca gagtgattcc
     4561 ggagcgagtt ttcaagaacc tggttgtcgt ctttgctgct gtggctgcga tcaaactctt
     4621 cttctgagca ggccagcccc tgcacttatc gcgtgtgaag acaagagcct cttggaagct
     4681 cagagtcgaa ctgtgttgcc tgacgttcgg ttggaacgcg atagagcatg aagaggtctt
     4741 gggaacgttt cgcttgacat tccaaggact tctgtccttg tataggcaaa cattagttag
     4801 tttagtgtga tatgggagaa tggaagaaat ggctaagcat ctgattctga tacctgtacc
     4861 gcaacgattg gtgtccctga aaggggagta cgagcttcca ctttcgggaa gcatcgtgtg
     4921 cctaggtgat ccctcgctga cgtttcccgt cgcccggatc atccaaacag acctcctggc
     4981 gatgcagaga gtcgagtggt cgctgcgttc tggatctgca ggcggcgctg tcggaagtga
     5041 actgacgatt cgtgttactg cgaagttgga tattcctgcc caagggtatt cactgcgagt
     5101 gacgcgaaca ggaattgagt tgaacgcatc cactgcccag ggtgccttct acgggtcaat
     5161 gaccttgaga cagattctga ggcaatgtga aggcgctttg cctgcctgta cgatcagcga
     5221 tcatcctgac tttgcagtgc gcggcgtgat gctggatgtc tgcagagaca aggttccaac
     5281 tctggaaacg ctcttccaat atgtagacga gttgtctgag ctcaagatca accagttgca
     5341 gttgtatacg gagcacactt tcgcctacag gaatcacccc gaagtgtggg cacaggcaag
     5401 tcctttgaca ggggaagaca tcctcaggct tgacgcttac tgtcgtgagc gttttatcga
     5461 actggttccc aaccagaact cctttgggca tatgcaccgt tggttcgaca tccccaagta
     5521 tttggatctg gctgaatgtc ccgaaggttc caccgctcct tggggtgggc attttggtcc
     5581 tttcactcta gatccaacca atcccaattc cctggcactc ctggaagatt tgtttgccga
     5641 tctgctacct catttcacca gcaggcagtt caatgtcgga tgcgatgaga ctttcgatct
     5701 tggccaggga cgaagcaaaa ccgcttgtga caagaagggc aaagaccgcg tctatctgga
     5761 atttctcctg aaaattcacc gactggttcg gaaacacggc aggaagatgc agttctgggg
     5821 tgacattatc cttcagcatc ctgatcttat tcccgaactt cccaaagatg tgattgcgct
     5881 caactgggga tacgacgcca atcatcccta cagcaagcag tgtcccttgt tcgccaaatc
     5941 aggaattccg ttctatgtgt gtcctggcac gtctacctgg gctgcgattg ccggtctttc
     6001 ggacaatgcg gttgccaaca ttcgaaacgc ggcggagaac ggtctcagga atggtgccct
     6061 tgggctgttg aacactgact ggggtgactg tggtcattgg cagtacctgc cggcgagcta
     6121 tgttgggttt gcagccggct ccgcgctctc atgggctttc aaagtcaaca gagatgtcaa
     6181 tctcgttgat gcgctagata tgcatgtttt taaggactcg agtcgttcaa tgggaagact
     6241 cgcctacgat ttaggcaaca caggtgttcg aaccaacaac cctgtcaaca accgacatct
     6301 attctggtgt ctgcagagac ctttcagtcc cgaagttctc aaggttggta caccgaagca
     6361 aatcaggtct gccatggctg agatcgaggc ctccatttgt tcccttgaaa gatcacggat
     6421 gaacagatct gatgcggcat tgatccgcga tgagtttgcg aatgctgcca gaatgcttct
     6481 tgacgcctgc aagcgaggtc ttgttgcgaa gcagaaggca gcttccagag gctctatcct
     6541 tgaactcgac tcagagatga gaatcatcct gggcgaacat cgccgcctct ggtgcgcgcg
     6601 caacagagtc ggtggtttgc aggacagcgc agcgattctc gagcaacgct tgagggagta
     6661 cggcgagctg ttgaaatgat ggagcccttg attctgaaac taacatccga tcatgtggat
     6721 tacctacgcg acgagtcgcg caggacggg
//
LOCUS       DAOQUC010000022         6743 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_12467_length_6743_cov_2.896382, whole genome shotgun sequence.
ACCESSION   DAOQUC010000022 DAOQUC010000000
VERSION     DAOQUC010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6743
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12467_length_6743_cov_2.896382"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /locus_tag="PKH07_00730"
     CDS             complement(<1..351)
                     /locus_tag="PKH07_00730"
                     /inference="COORDINATES: protein motif:HMM:NF013486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family N-terminal
                     domain-containing protein"
                     /protein_id="HNT33505.1"
                     /translation="MIKYEARQERFRRWMGREHFDGFLITNLTNVRYLCGFTGSNGTI
                     LMTQEEGIFLTDGRYRFQAADEVQGLPVKFAVGKSLPRQLKPLIPARIPFRLGFESDV
                     VTVVQHGFLLQELRS"
     gene            693..2732
                     /locus_tag="PKH07_00735"
     CDS             693..2732
                     /locus_tag="PKH07_00735"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNT33506.1"
                     /translation="MADAIATSRSDHSLWLSARSFILSGRTLLVVLIFVAYLPVFQAE
                     FVWDDDRFSSEGDFPTLEGLRSLWFAPQHSVMRSESGYWPLTYSTFWIERRIWGVHPL
                     GYHLANVAIHSANSLLVWHLLALLQVPGAVLAALLFALHPTRAESVAWVIERKDLLSA
                     LFSLASFRFYIVFHHRRCWRPLALSVLFFLCAMLSKSVALTLPVFFIVWFWWKGRLPA
                     DSDSAAYPGTPRQSNIHLLASLIPFFVISGVFGFLGLWLTRRQENLIPYTTVAERLII
                     AGRSFWFYVAKTVWPSNLIPIYPKWPVEPHAYTQYLFPLLAGMLLIGLFIARRRIGRG
                     SFAAMAFFGLTLSPTLGLIPFPLHEYSYVALRYGYLASAGLFALLSAVIWRFLRGLDE
                     RRRHTAQHLTLGCAIVLCVPLTWHHASQYHNMETLFKHCLRGNPNSLVAHNQLGIYYS
                     VSNQSDKAVETFKKALSIDPHSPDIHYNLALVFSNRGELEAAVDHYEKAIALRPEHSA
                     TADTHNNLARLLQQKGRREEARVHFERAIALRCGDPESAWAHINLANLLWSENQFHDA
                     ESHYRIAIDLKPSDPEIAEAHRNLASVLATQQRFNEAKEHYTRAIAMKPAAEESAVPY
                     TMLADIQSLQGQWHEAEASYRAALSLCRDPAKRRSIEQSLRDLAARRTDGEQGNE"
     gene            2887..3303
                     /locus_tag="PKH07_00740"
     CDS             2887..3303
                     /locus_tag="PKH07_00740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33507.1"
                     /translation="MNRERKKRILVADSDRARLTALTVILESDDFEVFAASDADTCVA
                     EARSRQPNLLILNTSFGSVQDDQSVLSAMKADSVLERIPILLLSEGRCNGSQAPADAS
                     DSSGRRREIKHPHDLIRQIDLLLRKAQGASFPEGRR"
     gene            3306..3860
                     /gene="thpR"
                     /locus_tag="PKH07_00745"
     CDS             3306..3860
                     /gene="thpR"
                     /locus_tag="PKH07_00745"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HNT33508.1"
                     /translation="MRTFIAVEVPGEIKQKVREVQSALQRSGFSLRWVQPENMHLTLV
                     FLGEVEGSLVSPLSEVLDEVTSRRQVFEIRIGGLGAFPNEKSPRVYWVGLLEGSSELC
                     ALQKEIQDGVRRLNIPLEKGRFHPHLTLGRCRDNRAPGAIPGSLREAVLGTTKVCAVH
                     LIRSQLGGSQPVYSTLHSAQMSQP"
     gene            3888..4370
                     /locus_tag="PKH07_00750"
     CDS             3888..4370
                     /locus_tag="PKH07_00750"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNT33509.1"
                     /translation="MNISIVRAESSHIHDCVSAVIHSEIGRVYGMSEDKMRNTLTRAM
                     DSGEVFVAVDPQNHCLGFIRVALRGAFCRFPYLMLIAVNEACRGQGVGNRLLSFFEEI
                     GFGEADRIFLLVSHFNTGARRLYELLGYKSVGVLPDLVIKGVDEILMMKCRPEELPAV
                     "
     gene            complement(4387..5934)
                     /locus_tag="PKH07_00755"
     CDS             complement(4387..5934)
                     /locus_tag="PKH07_00755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33510.1"
                     /translation="MNSGDTLTESRRKTKRFGTIANLWPLCLVNLLGSVCWAGNAATS
                     NYFAVQGPLYDGRPLPDSAIPRLQQLHGLGTLADFSFYYYRNRSPRGLAREIKARGFS
                     WLCGYSSRTTPELVEAFHDEGMAFVLSLWGTIIYSPGTLSPPPPDSGKQVFVGGAQAP
                     DANPVFYCPNSQEYVEWYRKMVHDQVHRVPADMIMMTESFLGEWGGPDATTYGCFCDS
                     CRSRFLQVYPEETNLPDFQNPASPRYWKTNRVLYEKWITFRADSVKKFQQATYDAVIQ
                     ELPNVAIAGNMLAIDDPQGISKVREYNAQDVNLLANAMPWDFFYLQAHFPDWLKISLD
                     AEQHIRSYVPFLTAIRSNAPKLRVGVTTDSGSNMGMRRSLAWLHACALAAKQDGFSSF
                     APYEYSISRFIYEEPPTVIGIKPDGAGKKLEVIFSKRVDSSTAQNTSNYTLDSGSNPS
                     QVQFDGGNIATLIFESWQRNRNASIRIVNVKDDWAGFWFKSHAGSGVQFQANTISPNT
                     VLDFYIP"
     gene            complement(5931..>6743)
                     /locus_tag="PKH07_00760"
     CDS             complement(5931..>6743)
                     /locus_tag="PKH07_00760"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNT33511.1"
                     /translation="QLRQIVMNLITNASEALGDRPGIISLRTGTQDWVPVGADEFQLG
                     QDIPEGRYAFLEVSDSGCGMDTEMIHRIFDPFFTTKFAGRGLGLASVLGIVRSHGGAL
                     RLTTQPGRGSTFRVLLPCSKEVSISTKDKRRDIGNWKGNGTVLVIDDEEIVRLLAEQA
                     LGRSGFRSLLAGDGAEGVQMFLDQGDEIVAVVLDLTMPGISGKETAQRILQIDKNARI
                     ILSSGYTEHEVLNELKGLPIASFIQKPYRSDELVLTLKSVLEQETSWTGENA"
BASE COUNT         1507 a         1709 c         1800 g         1727 t
ORIGIN      
        1 tgaacgaagt tcctgcagga gaaatccatg ctgaacgact gtcaccacat ctgactcgaa
       61 gccgagtcga aaggggattc gagcggggat aagaggcttc aactgcctcg gaaggctttt
      121 tcccaccgca aacttcacag gcaatccctg aacttcatcg gcggcctgaa aacggtaccg
      181 cccatctgtc agaaagatcc cttcttcttg agtcatcaat atcgtgccgt tggagccggt
      241 aaaaccgcag aggtatcgga cattggtcag attggtaatg agaaagccgt caaaatgctc
      301 tctccccatc catcttcgga aacgttcctg ccgagcttcg tacttgatca cctggtggac
      361 tccttgttct cagaaaccag attccggaga ataaggtttc tctcacgagc aggcactgca
      421 cagtgatgta catagcccat gcgccccaag gcgttagcct gcctcaaact gaatggaaga
      481 ataacacgaa ccgacaggct tcagaaggat ttactcactt gattctgtag tcatcatccc
      541 ttgcgcactt gcctcgttct gtgtttgaat gaactggtga gaacgatctc ctgtcgtttt
      601 cgccaaattg atcgagatca tgttgggaag gcaagatgtc ccgaaggaag tccaggtcag
      661 ttccgagagc aagagacaca acacgcagat gtgtggcgga tgctatagcg acgtcacgat
      721 ccgatcattc actgtggctc tccgcgcgat ctttcatcct ttcgggaagg actcttctgg
      781 ttgtactcat cttcgttgcg tatcttcctg tctttcaggc tgagtttgtt tgggatgatg
      841 atcggttttc ttcagagggt gactttccga cgctcgaagg tctgaggagt ctttggtttg
      901 cgccacagca ctcggtcatg cgctctgaat cgggatactg gccactgacg tattcgacct
      961 tctggatcga aagaagaatc tggggagtcc atccgctggg atatcatctc gcaaacgtgg
     1021 cgattcacag tgccaactct cttcttgtct ggcatcttct tgcgcttctc caggttccgg
     1081 gagctgttct tgctgcactt ttgtttgcct tgcacccgac gcgcgctgaa tcggttgctt
     1141 gggtcattga aagaaaggat ctgctttctg cacttttctc gctcgccagt tttagattct
     1201 atattgtctt ccaccacagg cggtgttggc gccctctggc gctttccgtg ctgtttttcc
     1261 tctgtgccat gttgagcaag tccgttgcgc taaccttgcc agtgttcttc attgtgtggt
     1321 tttggtggaa ggggagactt cctgcagact ctgattctgc agcataccca gggactccca
     1381 ggcagtccaa tatccatctt ctcgccagtc tgatcccgtt ctttgtcatc tccggagtat
     1441 ttggtttcct gggtctatgg cttacgagac ggcaggagaa tctcattccg tacaccaccg
     1501 tagccgagag gctgatcatt gccggccgtt ccttctggtt ttatgtcgct aagacagtct
     1561 ggccatccaa tctcattccg atctacccga aatggccagt tgagccccat gcgtatacgc
     1621 aatacctgtt ccccctattg gccggtatgt tgctgattgg actgtttatt gctcggcgaa
     1681 ggatcggcag aggctctttt gctgcgatgg cgttctttgg actgacgctc agtccaacct
     1741 tgggtctaat cccttttcct ttgcatgaat actcctatgt tgctttgaga tacggctatc
     1801 tggcgtcagc cggtttgttt gctttgttgt ctgctgtcat ttggcggttt ctgcgaggcc
     1861 ttgatgaaag acgacggcac acggcgcaac atctgacgct tggatgcgcg attgttctct
     1921 gcgtcccgct gacctggcat cacgcttcgc agtatcacaa catggagact ttgttcaaac
     1981 actgcctgag aggaaatccg aacagtctgg ttgctcataa ccagttgggc atctactact
     2041 cggtgtcgaa tcagtctgac aaggctgtcg agacgttcaa gaaggctttg agtattgatc
     2101 cgcacagtcc cgatatccac tacaatctgg ctcttgtctt ttccaacaga ggcgaacttg
     2161 aggctgctgt cgatcattat gaaaaggcca ttgctcttcg tcctgaacac tctgccactg
     2221 ccgacactca taacaacctt gctcgactgc ttcagcagaa aggtcgtcgc gaagaggctc
     2281 gggtgcattt cgagagagcc attgcgcttc gctgcggtga tccggaatct gcctgggcac
     2341 acatcaacct tgccaatctc ctttggtctg agaatcagtt tcatgatgca gagtcccatt
     2401 acaggatcgc gatcgatctc aagcccagcg atccggagat tgctgaggca cacaggaatc
     2461 tggcgagtgt tctggcgacg cagcaacgat tcaacgaagc caaagagcac tatactcgag
     2521 cgatcgcgat gaaacccgca gctgaggaat cggctgttcc atacaccatg cttgctgaca
     2581 ttcaatctct tcagggacag tggcacgagg cggaggccag ttaccgtgct gctttgtctc
     2641 tgtgtcgaga tccagcaaag cgtcgatcaa tcgagcaaag tctgagagac cttgctgcca
     2701 gaaggactga tggagagcag gggaacgagt agagtgttcg cttcttgctc tgttgcgcaa
     2761 tgactgagaa atgtgcttgt attcatcaca aaagaaggcg tatcggtgtc gcgaaactct
     2821 ctgcttttca gagaaggagt ctggtgagaa ggcacgctat ccgaatggga gtcgaggatc
     2881 aatctcatga accgtgaaag aaagaagagg attctggtgg ctgacagtga cagagcacgt
     2941 ctcaccgccc tgactgtcat tctcgaaagc gacgacttcg aagtgtttgc ggcttcggac
     3001 gccgatactt gcgtggcgga agcccgctcc aggcaaccga atctgcttat actcaatacg
     3061 tcatttggga gtgtgcagga cgaccagagt gtgctgtcag ccatgaaagc ggattctgtc
     3121 ctggagcgca taccgattct gctcttatcc gagggacgct gcaatggcag ccaggctcct
     3181 gcggatgctt ccgattccag cgggcgtcgt cgagagatca agcatcctca cgatctcatt
     3241 cggcagatag atttgcttct gcgaaaagcg cagggagcca gcttccctga aggcaggaga
     3301 taagcatgag aacgttcatc gccgttgaag ttccaggaga gatcaagcag aaggttcgag
     3361 aggttcagtc agcgctgcaa cgttccggtt tcagtctgcg ttgggttcag cctgagaata
     3421 tgcacctgac tctggtcttt ctgggtgagg ttgaggggag tcttgtttct ccactgtctg
     3481 aggttctgga tgaagtcact tcgaggcgtc aggtgttcga gatcaggata ggtggactcg
     3541 gtgctttccc aaatgagaaa tcgccgaggg tctattgggt gggtcttctt gagggttctt
     3601 ccgaattgtg cgctcttcag aaggagatcc aggatggggt gcgtcgtctg aacattcccc
     3661 ttgagaaggg cagattccat cctcatctga ccttgggcag atgcagagac aaccgagcgc
     3721 caggagccat tcctggaagt cttcgcgaag cagtgcttgg cacgacgaag gtgtgtgctg
     3781 tgcatctgat ccgcagtcaa ctgggtggta gtcaacctgt ctatagtacg ttgcactctg
     3841 cgcagatgtc tcaaccatga ctcagttggg gtgataggga ggaattcgtg aatatctcga
     3901 tcgtcagggc ggaatccagt cacattcatg actgtgtcag tgctgtgatc cattcggaga
     3961 tcggtcgcgt ctacggcatg tcagaagaca agatgaggaa tacgttgacg cgtgctatgg
     4021 acagcggaga ggtttttgtg gcagtagacc cgcaaaacca ctgcctggga tttatccgtg
     4081 ttgcactcag gggtgcattt tgtcgctttc cctacctgat gctcattgcg gtgaatgagg
     4141 catgtagagg gcagggagtt ggcaacaggt tgctttcctt ctttgaagag atcggttttg
     4201 gggaagcgga cagaatcttt ctgctcgtga gccacttcaa cacaggggca agaagactct
     4261 acgaactgct tggatacaaa tcggtcggag tgcttccaga tcttgtcatc aagggtgtag
     4321 atgagattct catgatgaag tgccgtccgg aagaactacc agcagtgtga gttttctcgc
     4381 cgaggactac ggaatgtaga agtccagtac tgtgtttgga ctgattgtat tggcctgaaa
     4441 ttggacaccg cttcctgcat gagacttaaa ccagaatcct gcccagtcgt ctttcacatt
     4501 cacgatgcga attgaagcat tcctatttct ttgccagctt tcaaaaatca gcgtggcaat
     4561 gttgccaccg tcgaattgga cttggctggg atttgatcct gaatcgagtg tgtagtttga
     4621 agtgttttgt gctgtcgagg aatccactct ctttgagaaa atgacttcca actttttgcc
     4681 cgcaccgtcc ggcttgatcc cgataaccgt aggtggctcc tcgtagatga atcggctgat
     4741 gctgtactcg tagggagcaa aggaggagaa tccatcttgc tttgcagcca aggcacaagc
     4801 gtgaagccac gcgagagaac gcctcattcc catattggag cctgaatcgg ttgtgacacc
     4861 aactctcagt ttgggggcat tgctgcggat cgctgtgagg aagggaacat aactgcggat
     4921 gtgttgttct gcatccaggg atattttcaa ccagtctggg aaatgagcct gaaggtagaa
     4981 gaagtcccac ggcattgcgt tcgccagaag attcacatcc tgtgcattgt actcccgaac
     5041 cttcgagata ccctgtggat cgtctatcgc aagcatattt ccggcgatgg caacgttggg
     5101 gagttcctgg atgacagcat cgtaggtggc ctgctgaaat ttcttgaccg aatcggcacg
     5161 gaaagtgatc cacttttcat agagtaccct gtttgttttc caatacctgg ggcttgctgg
     5221 attctggaag tcgggaagat ttgtttcctc tggatagacc tgaaggaaac gcgaacgaca
     5281 agagtcacaa aagcatccat aggttgtcgc gtccggtcct ccccattcac ccagaaaact
     5341 ctccgtcatc attatcatgt cggctggcac tcgatgtacc tggtcatgca ccatctttcg
     5401 ataccattcg acatactcct ggctgttggg gcagtagaag acgggattgg cgtcgggagc
     5461 ctgagcgcca ccgacgaaaa cctgttttcc cgaatcagga ggcggtggtg aaagtgttcc
     5521 cggactgtag atgatcgttc cccagagaga cagcacaaaa gccatgcctt catcatggaa
     5581 ggcctcgacc agttcaggag tggttctgga cgagtagcca cagagccagc tgaaccctct
     5641 cgctttgatt tccctggcca aacctcgcgg gcttcgattg cgatagtagt agaaagaaaa
     5701 atcggcaaga gttcccagtc catgcaattg ttgcagtcgg gggatggctg aatcaggcaa
     5761 tggacggccg tcatagagag gtccctgaac agcgaagtag tttgatgtgg cggcatttcc
     5821 cgcccaacaa acagacccaa gaagattgac aaggcaaaga ggccaaaggt tggcgatggt
     5881 gccaaagcgc ttggtcttgc gtcgggattc agttagggta tcgccgctgt tcatgcgttc
     5941 tctcctgtcc acgatgtctc ctgttccaat acactcttca gagtcaggac gagttcatca
     6001 gagcggtagg gcttttggat gaaactcgcg attggcagcc ccttgagttc attcaagact
     6061 tcatgttctg tgtatccact ggagaggatg atccgagcat tcttgtcgat ctgaagaatc
     6121 ctctgtgcag tttccttgcc actgattccg ggcattgtca aatccaggac gaccgccaca
     6181 atctcatcgc cttgatccaa aaacatctgg actccctccg cgccgtctcc agcgagcagc
     6241 gatcggaatc cagatctgcc cagcgcctgc tctgccagca gacgaacgat ctcttcatca
     6301 tcaatcacca ggacagtgcc attgcccttc cagttgccaa tgtcccttcg tttgtctttc
     6361 gttgagatag agacctcttt ggaacaaggg agcaagaccc gaaaggttga acctcggccg
     6421 ggttgtgttg tgagtctcaa ggcaccaccg tgacttctga cgatacccag caccgacgca
     6481 agtcccagac cgcgtcccgc aaactttgtc gtgaagaagg gatcgaatat cctgtggatc
     6541 atctctgtgt ccataccgca gcctgaatct gacacctcaa gaaacgcata tctgccttcc
     6601 gggatgtcct gaccgagctg aaactcgtct gcgccaacgg gcacccaatc ctgtgtaccc
     6661 gtcctgagtg atatgatacc agggcggtca cccagcgctt ctgatgcatt ggttatcagg
     6721 ttcatgacga tctggcgcag ctg
//
LOCUS       DAOQUC010000023         6695 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_12580_length_6695_cov_3.583283, whole genome shotgun sequence.
ACCESSION   DAOQUC010000023 DAOQUC010000000
VERSION     DAOQUC010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6695
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12580_length_6695_cov_3.583283"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1386)
                     /locus_tag="PKH07_00765"
     CDS             complement(<1..1386)
                     /locus_tag="PKH07_00765"
                     /inference="COORDINATES: protein motif:HMM:NF024120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HNT33512.1"
                     /translation="MRISGFAFLFSIGVLFTVVTGYSQETSTPGSVYDVKAYGAVGDA
                     TTDDTAAIQRALDDAGAARGGIVWLPSGNYMVRGHLNIPSHVTLEGIWTAPPAWSAMA
                     GSTLLAVEGRGDENGTPFITLQPAATLKGVTIYYPEQKSDDIQPYPWCIAGIRDNPSI
                     IDVMLINPYLGVDFGNTRHEAGGVGRFLIRNLYGQPLRKGIFIDGCLDVGRIENVHFW
                     PFWQGAGYTREHGEAFIFGRTDWQYVLNTFCWGYKIGYKFVNVGQGPCNGNFVGIGAD
                     FAEYAVYAESASDLGVLITNGEFVSFGGKDSVEVVTTPDFIGTLQFQNSAFWGPAKMI
                     ARLDGKGTVTFDNCNFRHWGYIDTPQFAIDCLGGNLIINSSHFFQSCPKVWLREGVRK
                     AVITGNIGSGIEGILNESNGSVVIDSNAFDGIPDEGKNTVVVDNSMPWGASRTGAFEC
                     SEGWGKEDRNDS"
     gene            complement(1472..3412)
                     /locus_tag="PKH07_00770"
     CDS             complement(1472..3412)
                     /locus_tag="PKH07_00770"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HNT33513.1"
                     /translation="MISLKNVHLRLGGRPVLKGVDIQIREGECLAVVGPNGCGKSTLL
                     KLVAGTMRPDSGEISRPTRSTIGYLPQEALFDSTHSLETELSSAFEQLQHAFREMSDL
                     EHQMAHTDPNSSDYERILERYGELSHLVEHQDGYSFESQVRRVATGLGFRQEDLSRSC
                     QEFSGGWKMRILLAKLLLERPDILLLDEPTNHLDLESMLWLEEWIRGSGRTVIMVSHE
                     RAFMDRLVDRVVCLETGEGDAYTGNYTNYLKQSEIKRQQRLQAYERQQQEIEQMEAFI
                     RKFRYNAARASLVQSRVKQLEKIERLPPPFYGEAIYFAFPEAPPSYREVIQLEGLGKS
                     YGDLQVFADVNLAIYRGQRIGLVGPNGAGKSTLMRILAGREKPTSGTFTLGRGVSVAY
                     FAQEDTGTLTSEQTLLQAIEVSAPPGQAQRGRDLLGAFLFSGDEVEKPLSALSGGERT
                     RFRMAQMLFSPANLLLLDEPTNHLDVTSRATVEKALKTYSGTVVVVSHDRVFMERVTD
                     RIVEIKDGGIRTFPGNYGEYLEYERSMVIGDNSQPKESAQIVAQTKPERKQEYEENKA
                     RNRAIRSLQRRIASLEEKIEEQETLIAEIRQRMEDPRIATDYTQLASLTQQHTEETAK
                     NLETLKEWEQLTNELETFSGER"
     gene            3637..3710
                     /locus_tag="PKH07_00775"
     tRNA            3637..3710
                     /locus_tag="PKH07_00775"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3669..3671,aa:Cys,seq:gca)
     gene            3808..5196
                     /locus_tag="PKH07_00780"
     CDS             3808..5196
                     /locus_tag="PKH07_00780"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HNT33514.1"
                     /translation="MVDPRIPKYIESLALMQKGNFQVEIPVGEQDEIGSLGLGLRTLG
                     ETLKREFDEINVMTRITEQINAGLTLEEVLNLVYDSFRSLLPYDRIGFSLIEPDGENA
                     TAVWARSDGTEMHITKGYSAPLRGSSLAEIIRTGKPRILNDLEQYLREHPESESTRKV
                     LKEGIRSSLTCPLIAKGKPVGFLFFSSKQLNAYRDAHVESFLGIAGQLALIVEKGRLY
                     ERLVALNEIKNRFLGIAAHDLRNPIAGILGYTSILEKGLLGALPDQQMKIVGRIGSIC
                     TRMQKMVEDLLDVSAIESGNLDLKREWIALDAFLQEQFETARMLAQVKNIEMRMEIPS
                     DLPRIFVDSGRLAQVISNLVTNAIKFSQSGTVVTLLARVENGEALIAIKDQGPGIPKD
                     ELGNLFRDFGRTSVKPTDGEKSTGLGLAIVKRLVEAHGGRIWVESQVGAGSTFVFALP
                     LQSEHTDSGTTR"
     gene            5193..>6695
                     /locus_tag="PKH07_00785"
     CDS             5193..>6695
                     /locus_tag="PKH07_00785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33515.1"
                     /translation="MILRRAFVLLASLRLAVIVLLALSFAMAWGTILEAQSGSEAARR
                     LVYAASWFDGLLVLLAANVLCAALKRYPWRRRHVGFLITHAAILIILAGTFLTRHFGV
                     EGTITLAEGEAASAFSVDEDALVIEHRGSGKSWEFLPGFGLSRVMNLSWDESEAMKEL
                     RLQVKEIIPHALPVESVSGGGETFNPAVAVEVPEQDHPSWIFANDPDKNRIVLGVVSV
                     ALQVCSTSEEIRPYLNPEETPSQGYPHGVVEITDSGGQQTILNVDPAQDEKPVPLGES
                     GWTAHIAEYLPDAVVSGGRLISRSERPENPAVRLIFTSPQGVTEERKLFARFPEFSSM
                     HRQATSSIPVEALYRFEQDSDSSVIRLLVLPGETLYVVVPDDQGTPSLQSVTVGQMIP
                     VAGDERRGIRVRAFHPRAQKVTEWISSPWEGDPPALRLTLGQGSEESSGWVGYGADPL
                     LLTLCEEFFSLRLSQREVPLGFQIRLLEFGNPTYGGTGMAARYYSTVDVSP"
BASE COUNT         1460 a         1812 c         1807 g         1616 t
ORIGIN      
        1 ggagtcgttg cggtcttcct ttccccagcc ttccgaacac tcaaacgcgc ccgttcggct
       61 tgctccccaa ggcatcgagt tatccacgac gacggtgttc tttccttcat caggaattcc
      121 atcgaaagca ttggagtcaa tgaccacact gccgttgctc tcattcagga tgccttcaat
      181 ccccgaaccg atattccccg tgatcacggc ttttctgacc ccttcacgaa gccagacttt
      241 cggacaggac tgaaagaagt gtgagctgtt gatgatcaag tttcccccaa ggcaatcaat
      301 ggcgaactgt ggagtgtcaa tataccccca atggcggaag ttgcagttgt cgaaagtcac
      361 tgttcctttt ccatccagac gcgcgatcat cttcgccggt ccccagaagg cgctgttctg
      421 aaactgcagc gttccgatga agtcaggcgt cgtgacgact tcgacggaat ccttcccgcc
      481 aaagctgaca aactcaccat tggtgattaa cacacccaga tcagacgcgc tctcggcata
      541 gacggcgtac tctgcgaagt cggcaccgat gccgacgaag ttcccgttgc acggcccttg
      601 accgacattg acaaacttat agccgatctt atagccccag cagaaagtgt tgagaacgta
      661 ctgccagtca gtgcgtccga agatgaacgc ctccccgtgt tctctcgtgt aacccgctcc
      721 ctgccagaat ggccagaaat gaacgttctc gatgcgtccg acgtcgagac atccgtcaat
      781 gaatatcccc tttcgaagag gctgtccgta gaggtttcgg atgagaaaac gcccgactcc
      841 acccgcctca tgccgcgtgt ttccaaagtc cacaccgaga taggggttga tcagcatgac
      901 gtcaatgatg gacgggttat ctctgattcc tgcgatgcac cagggatagg gctgaatgtc
      961 atcagacttc tgttcggggt agtagatcgt gacgcctttg agtgttgcgg cgggctgaag
     1021 cgtgatgaac ggagtgccat tttcatcgcc tctgccttcg actgcaagca gcgttgagcc
     1081 cgccattgcc gaccaggcgg gtggcgctgt ccagatgccc tcaagggtca catgggacgg
     1141 aatgttcaga tgccctctga ccatgtagtt accagaaggc agccagacga tgcctcctcg
     1201 cgccgcgcca gcgtcatcca atgctctttg gatggcggcg gtatcatcgg ttgtcgcatc
     1261 acccaccgcg ccataggctt tcacgtcata gacggagcct ggagtcgaag tctcttgact
     1321 gtatccagtg acaacagtaa acaacacacc aatcgagaac aggaatgcaa aaccggaaat
     1381 gcgcatcact tcgctccttt gactgtttcc ctctgacgta tacctacaga acaatccgag
     1441 tcattgcaaa cagattgtct gttcacatct ttcaacgctc ccctgagaaa gtttccaact
     1501 cgttggtcag ctgttcccat tccttcagcg tttcgagatt cttcgcagtc tcctcagtgt
     1561 gttgttgagt gagtgatgca agttgcgtgt agtcggtggc gattcgagga tcttccatcc
     1621 tctgacgaat ctctgcgatc agcgtttcct gttcttcgat tttctcttca agcgacgcaa
     1681 ttcgacgctg aagtgaacga atggctcgat ttcgagcctt attctcttcg tattcctgct
     1741 ttcgttcggg ttttgtctga gcgactattt gtgctgattc cttcggctgt gaattgtcgc
     1801 cgatgaccat cgatcgctcg tactccaggt attccccgta gttgccaggg aacgtccgaa
     1861 tgcccccatc tttgatttcg acaattctat cggtgacccg ctccatgaag actcggtcgt
     1921 gagagacaac aacgaccgtt cccgaatacg tcttcaacgc cttctcgacc gtggcgcgtg
     1981 acgtgacgtc caggtggttt gtcggctcat ccagaaggag aaggttggcg ggactaaaaa
     2041 gcatctgcgc cattcgaaaa cgcgttcgct ccccacccga cagggcgctc agtggtttct
     2101 caacctcgtc cccgctgaaa agaaatgctc cgagaagatc tcgaccgcgc tgtgcctggc
     2161 ctggtggtgc tgagacttct atggcttgca gaagtgtttg ctctgaagtc agcgttccag
     2221 tgtcctcctg agcaaagtag gcaacactca ccccgcgacc caacgtgaaa gtgcccgatg
     2281 ttggtttctc ccgaccggcg aggatgcgca tgagtgtgga tttgcccgct ccgttgggac
     2341 caaccagacc aattcgttga cctcgataga tagccagatt cacatcggca aagacttgaa
     2401 gatcgccgta tgactttcca agcccctcca actggatgac ttcgcggtaa ctcggtggtg
     2461 cttcaggaaa ggcgaagtag attgcctctc catagaaggg tggtggcagt cgttcgattt
     2521 tctcaagctg cttgacgcga ctctgaacca acgaagcacg tgctgcgttg tagcggaact
     2581 tgcggatgaa cgcttccatc tgctcgattt cctgttgttg gcgttcgtat gcctggagcc
     2641 gctgctgccg tttgatctca ctttgtttca ggtaattggt gtagttgcct gtgtaggcgt
     2701 ctccttctcc cgtctccaga caaaccacgc gatccacaag ccgatccata aatgctcgct
     2761 catgggaaac cataatcact gtacggccac ttccccgaat ccattcctcc agccacagca
     2821 tggactcgag atcgaggtga ttggtcggtt cgtccaagag caggatgtct ggtctttcca
     2881 gaagcagttt cgccagaaga attctcatct tccagccacc cgaaaactcc tgacacgaac
     2941 gggaaagatc ctcctgtcgg aacccgagac cagtggcgac acgccgaacc tgggactcga
     3001 aactgtaacc gtcctgatgc tccaccagat gggagagttc tccgtagcgc tccaggatcc
     3061 gttcatagtc tgaagaattc gggtccgtgt gtgccatctg gtgctccaga tcggacatct
     3121 ctcgaaacgc atgctgcagc tgttcaaagg cgctggacag ctcagtttcg agtgaatgag
     3181 ttgaatcaaa aagcgcctcc tgtggaaggt atccgatcgt ggagcgagtc gggcggctga
     3241 tctcacctga atcaggacgc atcgttcctg cgacaagctt gagcagtgtg gacttgccac
     3301 agccgttagg gccaacaacg gcaaggcatt cgccttcccg aatctgaatg tcaacacctt
     3361 tcagaacagg tcttccaccc aggcgcagat gcacattttt caaactgatc atgcgcgcat
     3421 ggtaaccaac ctgaaggctc ttgtcatcag tctgatgcaa attgtttgta tctgaatctg
     3481 tcgagacata tcctgtctcg agtgtatggc aggacgtgct gtcttcgagg cgttttggcg
     3541 tccaaacagg gcgccggtag agtcagcagt ccttcgcttt ctgtgccgaa gaacgctgtt
     3601 gactcaaacg ccaaatatgg tacgttattt tccagaggcg gcatagccaa gtggtaaggc
     3661 aggggtctgc aaaaccctca ccgccggttc aaatccggct gccgcctcca gtgcgcctgc
     3721 acatttccgc acggggcgct ctgcccactc caacgagttc cagttcgcgg aggaagtcag
     3781 gatgcccttc cgcaggagac cgatcagatg gtagacccac gtattcccaa atacatcgaa
     3841 tctttggctc tgatgcagaa ggggaacttc caggtagaaa ttcccgttgg agaacaggat
     3901 gagatcggta gtctcggtct gggtcttcgg acgttaggtg agacactcaa gcgggagttt
     3961 gacgagatta acgtcatgac ccgtatcacc gaacagatca atgccggcct gactctcgag
     4021 gaagtcctga acctggtcta tgactcgttt cggagtctgc ttccctacga tcgaattggg
     4081 ttctcgctca ttgagcctga tggtgagaat gcgacggcgg tctgggcacg ttctgacggc
     4141 actgagatgc acatcaccaa aggctattcc gcgcctcttc ggggaagcag tcttgccgag
     4201 attatccgca cagggaaacc gcgtatcctt aacgatctcg aacagtacct cagggagcat
     4261 cccgaatcgg aatccacaag gaaggttcta aaggaaggca ttcgttccag tttgacctgt
     4321 cccctcatcg ccaaaggcaa accggttggt tttctgttct tctccagcaa gcaactgaat
     4381 gcctatcgag atgctcatgt cgagtcgttt ctgggcatag cagggcagct ggccctcatt
     4441 gttgagaagg gtcgtttgta tgagcgtctg gtggcgctca atgagatcaa gaacaggttt
     4501 ctcggcatcg cagcacacga tttgaggaat cccatcgccg gcatcctcgg atacaccagc
     4561 attctggaga aaggtctgct cggtgcgctt cccgaccaac agatgaagat cgtcggacgc
     4621 atcggttcga tttgtacacg catgcagaag atggttgagg atctgttgga tgtcagcgcc
     4681 atcgaatccg gaaacctcga tctgaagcga gagtggattg ccttggatgc atttctacaa
     4741 gagcagttcg aaacggctcg aatgctcgcc caggtcaaga atatcgaaat gagaatggag
     4801 atccccagcg accttcccag aatctttgtg gattcgggtc gcttggcgca ggtcatcagc
     4861 aaccttgtca ccaacgccat caagttttcc caatcaggga ctgtggtgac gcttctggcg
     4921 cgggtggaaa atggggaggc tctcatcgcc atcaaggatc aggggccagg aattcccaag
     4981 gacgagttgg ggaatctgtt tcgggatttt gggcggacgt ccgtcaagcc gacggatggt
     5041 gagaagagca ccggccttgg tcttgccatc gtcaagcggc tggtggaagc gcacggtgga
     5101 cgcatttggg tcgagagtca agtgggtgcc ggttccacgt ttgtctttgc actgccactt
     5161 cagtctgagc acaccgattc cgggacaacc agatgatcct tcgaagggca tttgttctcc
     5221 ttgcatcctt gagactcgct gtcattgtgc ttcttgcgct gtcctttgcg atggcttggg
     5281 gaacgattct ggaagcgcag tctggttcgg aggctgcacg gcgtttggtc tatgccgcct
     5341 catggtttga cgggctgctt gtcctgctgg cagcgaatgt gctctgcgcc gcccttaagc
     5401 gatatccctg gcgaagacgt cacgtcggat ttctgatcac tcatgctgca attctgatca
     5461 tcctggctgg aacattcctg acgaggcact ttggtgtcga aggaacaatc actcttgcgg
     5521 agggtgaagc cgcatcggct ttctcggttg atgaggatgc gcttgtcatc gaacatcgag
     5581 gctctggcaa atcatgggag ttcttgcccg ggtttggcct cagtcgtgtg atgaatctga
     5641 gttgggatga atcagaggcc atgaaggaat tgcggctaca ggtgaaggaa atcatcccgc
     5701 acgccttgcc cgttgagtca gtctcaggcg gcggagagac tttcaatccg gcggttgctg
     5761 ttgaagtgcc ggagcaggat catcccagtt ggatttttgc caacgatcct gacaagaacc
     5821 ggatagtctt gggcgttgtt tcagttgcac tgcaggtctg ctcaacatcc gaagagattc
     5881 gtccatatct gaaccccgag gagacaccat ctcaaggata tccgcatggt gttgtggaga
     5941 ttaccgactc aggcggtcag cagacgattc tgaacgttga tccggcacag gatgaaaagc
     6001 ctgtgccatt gggagaaagt ggttggacag cgcatattgc ggaatacctt cccgatgccg
     6061 tggtatccgg tggacggtta atcagccgaa gtgagcgacc cgagaatccc gcagtacgac
     6121 tcatattcac ttcaccacag ggagtgaccg aggaaaggaa actctttgcg cgatttcccg
     6181 agttctcatc catgcatcgt caagccacgt cctcgatccc tgttgaggct ctgtatcgtt
     6241 tcgagcagga ttccgactct tccgtgatac ggctgttggt gctgccgggc gaaacattgt
     6301 atgtggtggt tcccgatgat caaggcacgc cgtccctgca gtctgtcact gtcggccaga
     6361 tgatccctgt tgccggagac gagagaagag gcattcgagt gagagcattt catcccagag
     6421 cacagaaagt gactgaatgg atttccagcc catgggaagg cgatccacca gcccttcgac
     6481 tgacacttgg acaaggctcc gaagagtcct caggttgggt tggctatggc gctgatccgc
     6541 ttctgctgac gctctgtgag gagtttttct cactgcgact gtcccaacgc gaagtgcctc
     6601 ttggttttca gattcggctt ctggagttcg gcaatccgac ttatggcgga acggggatgg
     6661 cggctcgtta ttacagcaca gtagatgtca gtccg
//
LOCUS       DAOQUC010000024         6666 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_12641_length_6666_cov_3.660112, whole genome shotgun sequence.
ACCESSION   DAOQUC010000024 DAOQUC010000000
VERSION     DAOQUC010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6666
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12641_length_6666_cov_3.660112"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..587
                     /locus_tag="PKH07_00790"
     CDS             <1..587
                     /locus_tag="PKH07_00790"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNT33516.1"
                     /translation="GWDGVRITDLGSTEGREVLGKSVQEVANERGQEPVDTALDLVVR
                     GNGYVGICTFSMNQEDTDKVLTHRLCSICTDASTRAPYGPLSKSCPHPRSYGTFPRFI
                     SEYVRDRKLVPMHEAVRRMCALPARQYRLKQRGMIREDYWADIVVLDLESLEDRACYG
                     NPHHYPAGVDYVVVNGKTVIRQGEHTDALPGVML"
     gene            643..2391
                     /locus_tag="PKH07_00795"
     CDS             643..2391
                     /locus_tag="PKH07_00795"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNT33517.1"
                     /translation="MKCDAIKRRDFLSHSLLGVVGLVAPSMFRFRKREMPNILWITSE
                     DNSSTFLGCYGNAFATTPNLDKMASEGILYENAFANAPVCAPARFTILTGMYACALGT
                     HHMRSRYPISDFVKPYPHYLRQAGYYCTNPGKTDYNFQTDDKSHWDEGDYTHRKKGQP
                     FFHVVNLNVSHESKIHNRNSELRHDPQKVILPPYHPDTPEIRADWAQYYDQVEKMDSQ
                     VGEILEKLEREGLAQDTIVFYFADNGGVLCRSKRMLYDTGTHVPMIIRFPKKYQHLAP
                     SKPATRTDRLVSFVDLAPSLLRLVGVDVPDYMQGEAFLGEGSAQPRKYVYLFRNRMDE
                     RYDMMRAVRDERYKYIRNYMPHRIYGQHLNYLWKAANTRSWEQEYKSGRCNRTQSLFW
                     QEKPSEELYDTRVDPHEVNNLAGDPDHADILKRMREEKMRWVREMRDPGFLPEGEMID
                     RSEGTTIYDLIHQDGFPADRIIETAEMASMRDARNLPEIIRRLDDEESAVRFWAATGC
                     VALGKAAEPASDALMSRLEDPCGDVRIAVSEALCILGKTEQLVTLENGNEMTRLHAEN
                     AAYALGLRSAQQETRQ"
     gene            complement(2401..3156)
                     /locus_tag="PKH07_00800"
     CDS             complement(2401..3156)
                     /locus_tag="PKH07_00800"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNT33518.1"
                     /translation="MGWLSAQMAGIFRRYECPVFQLHHVGVFSAPMSLSPQQFQLIMQ
                     SLKDLGCTTLSLEDYIDQLDLPRRPDQRQMVLTCDDALVGVYENAWPILREFGFQATI
                     FLPTDFIGGRSEWMTHIPGASQIPLLSWAQVEEMSKDPLISFGTQTNTHPDLTQVSPQ
                     RCHQEIKDSCAEIQEKLGIEAKTLVYPFGRFNDRVLNEVKQAGLLGACTDIQGVRNQW
                     RDRFQLKRVSILPTSSPRSLQMAFSPSFRALRG"
     gene            complement(3413..4942)
                     /locus_tag="PKH07_00805"
     CDS             complement(3413..4942)
                     /locus_tag="PKH07_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014551476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNT33519.1"
                     /translation="MKTNNSKHSGTQTLVTMMLCSAFLVVAPGSTVLGESLADRVVEH
                     RLDNGMTFLLLQRPEAPVLSAHIRFRAGGVNEPMGKTGIAHLLEHMAFKGTRHLGTNN
                     FRAEHRLLERIEQLGERLTAEIALGAKGDPEKIAAMREQLNQLHLEGDQYLVKEEIWD
                     TYLQNGAQGLNASTSKDVTSYYVSLPSNRLELWALIESQRMMESVMREFYRERDVVAE
                     ERRRGYEDRPDGKLYERFISAAFIAHPYGNPTIGWMSDIQTLTIRDAESFFNHYYVPS
                     RAVAAIVGDIDIEKTVAIVEKYFGRIPSKPEPLEQITQEPEQGGEKRIVVEFDAEPQI
                     LIGFQKPTLPEFDDYVFDVIDGLLTRGRTSRLYTRLVKQDQVATRVYSFGAPGSIYPN
                     LFVIGSAPRHPHTSEEVEKIVFEELGRMAQENVTERELLKVKNQLKADFLRDLRSNNG
                     LADKLSYFEVVGAGWRYPIEWVDVIERVTPDDVRRVASQYFSRSNTTVATLMRKVAAE
                     N"
     gene            complement(5027..6013)
                     /locus_tag="PKH07_00810"
     CDS             complement(5027..6013)
                     /locus_tag="PKH07_00810"
                     /inference="COORDINATES: protein motif:HMM:NF014474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HNT33520.1"
                     /translation="MPANLSPEYIEAERRYRAAKSHEERLSCLEEMLSKIPKHKGTEK
                     MQAGIRRKISQLKQQAEAQNRKRKGHSYKVNAEGAGQIVLVGAPNTGKSALLAAVTNA
                     EPEVADYPFTTRAPMPGMMPYLDIRIQLVDLPAISSEHLEGWVADVIKAADGVLIFCD
                     LASDDPVVQIDESVALLRDFGIELLPPTQEPVPELRGNQKKAIIAANKIDLPDTREMF
                     DLVSEMQTSLLPILAVSCVSGQGLETLKHQVFELLKIMRVYSKQPGKPPDMNEPFTIH
                     RGANLMEFAGVVHKDFVEQLKYGRVWGSGKFEGQIIKHDHILQDGDIVELHL"
     gene            6201..>6666
                     /locus_tag="PKH07_00815"
     CDS             6201..>6666
                     /locus_tag="PKH07_00815"
                     /inference="COORDINATES: protein motif:HMM:NF012899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HNT33521.1"
                     /translation="MGIIVQKFGGTSMGRWDRMDTDRLLQSAERVAKTKAQGHDVLVV
                     VSAMAGLTNLLLELAGRVNPEGEPREFDTLASTGEQISIGLMALALQRLGYPSISFTG
                     AQIGMKTDAAFTKARIQSIDTDRIRKAFRHGKIVVVAGFQGVTDEGDITTLGR"
BASE COUNT         1576 a         1786 c         1781 g         1523 t
ORIGIN      
        1 tgggatggga cggagtgcgc atcaccgatt tgggaagcac cgaaggaaga gaagtgcttg
       61 gcaagagtgt tcaggaagta gccaacgaaa gagggcagga accggttgac acggcgcttg
      121 atctggtcgt tcgaggcaac ggctacgtgg gcatttgcac cttttccatg aatcaggaag
      181 acacagacaa agttctcact catcgcttgt gttcaatttg caccgacgcc tcaactcgcg
      241 caccgtacgg accgctttca aagagttgtc ctcatcctcg ttcctatggc acatttcctc
      301 gtttcatcag cgaatacgtt cgggacagaa aacttgttcc gatgcatgaa gcggttcggc
      361 gcatgtgtgc gttgcctgcc cgccagtatc ggctcaaaca gcgtgggatg atccgggaag
      421 actactgggc ggatatcgtc gtgcttgatc tcgaatcgct ggaggatcgt gcctgctacg
      481 gtaatcctca ccattacccc gcgggtgtgg actatgtcgt ggtcaacgga aagacggtca
      541 tccgacaggg cgagcacaca gacgcccttc caggagtaat gctctgagcg gcaagagagc
      601 aggcctcgga gagcggaaga gatcgtttgg cggggtgact tcatgaagtg tgacgcgatc
      661 aagagaagag attttctctc gcattcgctc ttgggtgttg ttggcttggt cgcacccagt
      721 atgttcagat tcagaaagcg tgagatgccc aatatcctct ggatcaccag tgaggacaac
      781 agcagcactt ttctgggctg ctatggaaat gcctttgcca ccactccgaa cctggacaag
      841 atggccagtg aaggcattct gtatgagaac gcctttgcca acgctcctgt ctgtgctcca
      901 gcccgattca cgattctcac tggaatgtac gcctgtgctt tgggaacgca tcacatgcgc
      961 agcaggtacc cgatatccga cttcgtcaag ccctatcctc actatcttcg ccaagcgggt
     1021 tactactgca ccaatccagg aaagaccgac tacaattttc aaaccgatga caagtcccat
     1081 tgggatgaag gagactatac ccatcgcaag aagggacaac cgttcttcca tgtggtgaat
     1141 ctcaacgtct ctcacgaaag caagatacac aacagaaact ccgaactcag acacgatcca
     1201 cagaaggtca tcctgccgcc ctatcatcct gacacgccgg aaataagagc cgactgggcg
     1261 cagtactacg accaggtgga gaagatggac agccaggtcg gagagattct ggagaagttg
     1321 gaacgagagg gtctggcaca agacacgatc gtcttctact ttgcggataa cggcggtgtg
     1381 ctgtgcagaa gcaagagaat gctctatgac acgggcacac acgttccgat gatcatccgc
     1441 ttcccaaaga agtatcagca cctggctccg tcgaaacctg caacgcgaac cgaccgactt
     1501 gtcagctttg ttgatcttgc tcccagtctg cttcgccttg tgggagttga tgttcccgat
     1561 tacatgcaag gtgaggcgtt tttgggagaa gggagcgcgc aaccgcgcaa gtatgtttac
     1621 cttttccgca atcgcatgga cgagcgatac gacatgatgc gagcagtcag agacgagaga
     1681 tacaagtaca tccgaaacta catgccccat cgaatctatg ggcagcacct taactacctc
     1741 tggaaagccg ccaatacgcg atcgtgggag caggagtaca agtcggggcg ctgcaacagg
     1801 acgcagagcc ttttctggca ggagaagccg tcggaggaac tctatgacac acgtgtagat
     1861 ccccatgagg tgaacaatct cgccggtgat cccgaccatg cggacattct caagcgaatg
     1921 cgcgaggaaa agatgcgctg ggtgcgagag atgagagatc cgggcttcct tccggaagga
     1981 gagatgatag accgaagcga gggaacaacc atatacgacc tcatccatca ggatggtttt
     2041 cctgcagaca gaatcatcga aacagccgaa atggcaagta tgagagacgc gaggaatctt
     2101 cccgagatca tccgacgact ggacgatgag gagagtgcgg taaggttctg ggcagcgacc
     2161 ggatgcgtgg cgcttggcaa ggctgccgaa cctgcttcgg atgctttgat gagtaggctg
     2221 gaagacccat gcggggacgt ccgaatcgcc gtctcggaag ctttgtgtat cctcggcaag
     2281 acagaacaac tcgtcaccct cgagaacggc aatgagatga ctcgccttca tgcggaaaat
     2341 gcagcctatg ctttgggatt gagaagcgct cagcaagaga caagacagtg accggcttct
     2401 ctatcccctg agtgcgcgaa acgacggaga gaaggccatt tgcaggctcc tgggactact
     2461 ggtcgggaga atggaaaccc tcttcaactg gaatctgtca cgccactggt tgcgaacacc
     2521 ctgaatatct gtgcatgcgc caagaaggcc agcctgtttg acttcattca agacccgatc
     2581 attgaaacga ccgaaaggat agaccagtgt cttggcttca ataccaagtt tttcctgaat
     2641 ctcagcgcag gaatctttga tttcctgatg acaacgctgt ggtgaaacct gcgtcagatc
     2701 aggatgtgtg ttggtctgtg tgccaaagga gatgagcgga tctttggaca tctcctcaac
     2761 ctgcgcccag ctcagcaggg gaatctgaga agcgcctgga atgtgggtca tccactccga
     2821 acgcccgccg atgaaatccg tcggcaggaa tatcgttgcc tgaaaaccga actctctcaa
     2881 aatcggccag gcattctcat agacaccgac caaagcatcg tcgcacgtca gcaccatctg
     2941 tctttgatct ggcctcctgg gcaaatccag ttgatcgatg tagtcttcga ggctgagcgt
     3001 ggtgcacccc agatccttga gtgactgcat gatcagttgg aactgctgcg gagaaagact
     3061 catcggcgcg gagaaaactc cgacgtgatg cagttgaaag acagggcact catagcggcg
     3121 aaatatcccg gccatctgag ccgagagcca ccccattaca gacttgtttt cctcagacac
     3181 aggttccttc ccgcagcaac agttttgacg taaaaaagac ttccagacct ttttcaggct
     3241 ccatcacgaa acaaaacgat tcctttcatg cagaagtaga tttctcaata cttctggttt
     3301 tgggcaccgc caaagccatt gtcatcacgt ttcctgctcg ggcggcggca ccgccacaag
     3361 cccctgtcgc gattctctca tgcctcagcg gtgccctctt gtgcacaaac gtttagttct
     3421 ctgcggccac ctttcgcatc aacgtggcaa cagtggtatt gcttcgactg aaatactgcg
     3481 aggcaacgcg acgcacgtcg tccggcgtga cgcgctcgat cacatccacc cattcgatag
     3541 ggtagcgcca accggcaccg accacttcga agtaactcag tttgtccgcc agaccgttgt
     3601 tggatcgcag gtcacgcagg aaatccgctt tcagctgatt cttgactttg agcagctccc
     3661 gttcggtgac gttctcctga gccatgcgac caagctcctc aaagacgatc ttctcgactt
     3721 cttccgacgt gtgcgggtgt ctgggagcgg aaccgatgac aaacagatta ggatagattg
     3781 agccgggtgc tccaaacgaa tagacacgcg tcgccacctg atcctgcttc accagtcggg
     3841 tgtaaagacg cgaggtgcgt ccccgagtca acagtccatc tatgacatcg aagacatagt
     3901 cgtcaaactc cgggagtgtt ggtttctgaa aaccaatcag tatctgcggt tcggcatcaa
     3961 attccacaac gatacgcttc tcccctcctt gttccggttc ctgcgtgatc tgttcgagag
     4021 gctccggctt ggacgggata cgaccaaagt acttctcgac aatggcgacg gttttctcga
     4081 tatcaatatc cccgacgatg gcagcgaccg cccgcgacgg aacatagtag tggttgaaaa
     4141 aagactcagc gtctcgaatg gtgagggtct gaatatccga catccagccg atggtgggat
     4201 tcccgtatgg atgcgcgatg aacgctgcgc tgatgaaacg ttcataaagt tttccatcag
     4261 gtcggtcttc gtagccgcgg cgcctctcct cggcaacgac atcgcgctct cggtagaact
     4321 cgcgcatgac agactccatc atccgttggg attcgatcaa cgcccacagt tcgagcctgt
     4381 tcgaaggcag gctgacatag taggaggtga catccttgct ggtggaagca ttgagacctt
     4441 gtgcgccgtt ctgaagatag gtgtcccaaa tctcttcttt gaccaggtat tgatctcctt
     4501 cgagatgcag ttggttcagc tgttcacgca tcgccgcaat cttctcggga tctccttttg
     4561 cgccaagggc aatctctgcg gtcagccgtt cgccaagctg ttcaattctc tcgagcaatc
     4621 tgtgttcggc tctgaagttg ttggttccca gatgcctggt gcctttgaat gccatgtgct
     4681 ccaggagatg agcgatacct gtcttgccca tcggctcatt gacaccaccc gcccgaaacc
     4741 gaatgtgtgc cgagagtact ggggcttcgg gtctctgaag aagcagaaac gtcattccgt
     4801 tgtcaagtcg gtgctcgacg acgcggtcgg ccagtgattc ccccagaacc gtagacccgg
     4861 gtgcaacgac aagaaaagca gagcacagca tcatcgtcac aagagtctgc gttccggaat
     4921 gttttgagtt gttggtcttc attctgttcc tttcggagag aaaaggcttt cagcctctct
     4981 ctttgctccg ttacgaggcc aaatgtctcg tccgccaata gcgcatctaa agatgcaact
     5041 ctacaatatc tccatcctga agtatatgat cgtgtttgat gatctgtcct tcgaattttc
     5101 cgcttcccca aacacgaccg tacttcaact gctccacaaa gtccttgtga accacaccgg
     5161 caaattccat cagattggct ccacgatgta tcgtgaaagg ctcgttcatg tccggtggct
     5221 tgcccggctg tttggaatat acccgcatga tcttcaggag ctcaaagacc tggtgcttga
     5281 gtgtctccaa cccctgaccg gaaacgcatg aaaccgccaa aatgggcagt agagaggtct
     5341 gcatttcaga gaccagatca aacatctccc gagtgtccgg cagatcgatc ttgttggcgg
     5401 caataatcgc cttcttctga ttgccccgaa gctcgggaac aggttcttgc gtcggtggaa
     5461 gcagttcaat tccgaaatcc ctgagcagcg caacgctttc atctatctgt acgacaggat
     5521 cgtcactggc aagatcgcag aaaatcagaa cgccatcggc tgctttgatc acatcggcaa
     5581 cccagccctc aagatgctct gaactgatcg cgggcaaatc caccagttga attcgaatat
     5641 ccaggtacgg catcatccca ggcatcggtg ctctggtggt aaaggggtag tcggccactt
     5701 ctggttcagc gtttgtgacc gcggccaaca gagcggactt tcccgtgttg ggagcaccaa
     5761 caagaacaat ctgcccagcg ccttcagcat tgaccttgta cgaatgtcct tttctctttc
     5821 ggttctgtgc ttccgcctgc tgcttgagtt gggagatctt ccttcggatt cccgcctgca
     5881 tcttctcggt gcccttgtgc ttgggaatct tgctgagcat ttcctcaagg cacgagaggc
     5941 gttcctcatg gctcttggca gcacggtatc gtcgttctgc ttcgatgtat tccggtgaca
     6001 gattggctgg catcggttcc ttccaacatc ggtcgtatat tcctggcgaa tcgtccgcca
     6061 agagggattc tagtggagcg tttcagaaag cccaagaaaa atttccgaaa aagtaccgaa
     6121 catgctatag tcctcattca catttttatg cctgaaaaga ctgtcgggca aggtgacttc
     6181 ttcgaaaggg agaagaacgg atgggcatta tcgttcagaa attcggtggc acctcgatgg
     6241 ggcgctggga tcgcatggat accgaccggt tgctgcagtc cgcggagcgc gtcgccaaga
     6301 ccaaagccca ggggcacgat gtcctcgtcg tggtttctgc catggcgggc ttgacgaatc
     6361 tgcttctgga acttgccggc agggtgaatc cggagggaga gccgagagag tttgatacgc
     6421 tggcatccac cggcgagcag atttccatcg gtctgatggc tttggcgctt cagcgtcttg
     6481 ggtatccgtc catctccttc acgggcgcac agatcggcat gaagacagac gcagcattca
     6541 ccaaggcacg tatccagagc atagatacgg atcggattcg aaaagcattc cgacatggaa
     6601 agattgttgt tgtggcggga tttcaggggg ttaccgatga gggagatatc accacgcttg
     6661 gacgtg
//
LOCUS       DAOQUC010000025         6600 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_12813_length_6600_cov_3.062185, whole genome shotgun sequence.
ACCESSION   DAOQUC010000025 DAOQUC010000000
VERSION     DAOQUC010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6600
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12813_length_6600_cov_3.062185"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1513)
                     /locus_tag="PKH07_00820"
     CDS             complement(<1..1513)
                     /locus_tag="PKH07_00820"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33522.1"
                     /translation="MDTADHREDKVAMVPVSDAPLSLFAIPESGSLVPNLKNNVYLLV
                     SYPDGSPCKEAEVTVEYGAVDDRTRKQNTNDVGIAVLSVTPKVSEVTNLTIRAKDKEG
                     RTATKEFALDSKATAESLILRTDKALYQVGETIKAEVIATKKQGTFYLDMIREGQTVM
                     TYAGELQSGRALLAVDIDASMSGSLALQAYIFTPGTDLVRDTRLIYANPADALNIGIT
                     LDTETYKPGQSANIDFRVTDDKGNPITSALGISIVDEAVFALQEIHPGLEKVYFTLEQ
                     EIMKPRYEIHGYELDDLVRLPPQPGPIVPMRWNEDQQQAARVLLASAPSIPEPPIRVN
                     TFEEKQQQASEALLQRYQKDFERIHGVVQRYLQANSWKAPKNLLDAMIRGKYLRENEI
                     LDPWGNPYIFDFSQVEATRGYFTMKSSGSDEVIGTGDDLGAENMWFGVRGPRSMARRA
                     GVMEQEGIMLGAPVMDIAAAMPQAAGMAKGEMVADDLKASSGLAGAAAPEPVRL"
     gene            complement(1640..2797)
                     /locus_tag="PKH07_00825"
     CDS             complement(1640..2797)
                     /locus_tag="PKH07_00825"
                     /inference="COORDINATES: protein motif:HMM:NF013957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HNT33523.1"
                     /translation="MMSRSLIVVLGIVGLWTLMVGVGLAQERNEGWLIRESIEVTRVK
                     AGLRIACQLKTPQGSERTGELKFTLVDLDDKTLLEEKSLLDLASLDQKVSVVLRGNVE
                     DEKVPLCVLRYRLKMGKHVEEGSRSLMASLAQLETRIVAYRDLLAGTKASLRVVALNH
                     ATGRPVNDARVTISLIEKENARKLFEGKTRKNGSAMAQFDVPSDVEGTCQLKVTVEHE
                     RLGKDEVLQSVSVQRKAKILLTTDKPLYQPGQLIHMRTLCLAAGTLAPQAGKPLTFEV
                     MDSKGNKVFKKKTETDDFGIASAEFQLATELNLGTYIVRAILDSSQTEKNVDVQRYVL
                     PKFKVQVKTDRDFYLPGETLKGEVQADYLFGKPVSGGKVTVVASKFEIQFE"
     gene            3142..3873
                     /locus_tag="PKH07_00830"
     CDS             3142..3873
                     /locus_tag="PKH07_00830"
                     /inference="COORDINATES: protein motif:HMM:NF019430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HNT33524.1"
                     /translation="MKKMMILATLTVMLMPFAARAQEMQFRLPSPEVLEKLDLSQEQK
                     DQLKDIHSKVGDQVKQLQEKCREQANEIKEALGAAEPNKKAISKAAKQLGETRSKMLE
                     LQAESLADALVLLTPQQREQVKEWVKSHKGAGLTRGFGAREGIGQGRASEPNRREGLR
                     QRQGFGDDRGRGFRGEQGMRPEIRERIRERLQDMEPEKRAQMLRRFRQNWSPEGEEGR
                     GSGGQRMRDRQRDQDCPPESEPKED"
     gene            4202..4756
                     /locus_tag="PKH07_00835"
     CDS             4202..4756
                     /locus_tag="PKH07_00835"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HNT33525.1"
                     /translation="MKSKQETVTVRRLFAGVVFLCFLGMASGNLMAAELLGVRVYGKV
                     VNSAGYVIIADATVSLVSSNRVYVTTSDAEGDYEFTDVEVGFYQISATKEGFLPQTAE
                     LDVDGSKPEFLKNFFLELERSGDLNNDKIISAEDLMVFLKHWYAESPQASYNYLADFN
                     YDRIIDESDLLILLARWHQQTGGN"
     gene            4936..6111
                     /locus_tag="PKH07_00840"
     CDS             4936..6111
                     /locus_tag="PKH07_00840"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HNT33526.1"
                     /translation="METDCAQMQERLSNLRRTIHKCPELGFQEIETGKLIAKTLSDLG
                     LEVQTNVNKTGVVGVLKGAKPGRTIAFRADMDALPIQELNETPYKSQKPNVMHACGHD
                     GNTTILLGTAMLLCQQKDSLCGNVKFIFQPSEEAPPGGAKGMIEEGVLENPKVDAVVG
                     VHVDTDHPIGKASVRFGAHTANADRIHIVIKGRGGHAAQPHKCVDPIYISAMVIQAIH
                     GLPSRFVDPVEPFVVTLGKISGGDTFNVIPNDVEILGTVRTVTTETQDRVLEQIRLIV
                     ETIPKTYNGEGTIEVRRGYPVCINDAGISSLIQECCEAELGEDTVSVGTTPSMGGEDF
                     AYFAQKVPSSVLMVGVRNEELGMVYPWHHARFDMDERGLVIGAKCLARIATTFLCRD"
     gene            6131..>6600
                     /locus_tag="PKH07_00845"
     CDS             6131..>6600
                     /locus_tag="PKH07_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33527.1"
                     /translation="MITVKNTSIKRALWAFPLLLIFWLISTARAVEPAAHVSSATDEK
                     AQKLVKKVSALEDKIDLDYAEKKKQEILTKYPTLETDYLNFVRSKYPTLETEIIEIFK
                     KYPKPLKQYGTRNDTGKTKVTFELIEKMCMADGKCSDELGDLLSKKYPNFAKDSA"
BASE COUNT         1595 a         1686 c         1731 g         1588 t
ORIGIN      
        1 gaagccgtac cggttcagga gcagctgctc ccgccaagcc tgaggaagcc ttcagatcat
       61 cagcgaccat ttctcccttg gccattccgg ctgcctgtgg catcgccgcg gcaatatcca
      121 tcaccggcgc tcccagcatg atcccttcct gttccatgac acccgctcta cgtgccatgg
      181 aacgtggccc tctgacaccg aaccacatgt tttcggctcc aagatcatcc cctgttccga
      241 tgacctcgtc agagccggaa gacttcatcg tgaagtatcc cctcgttgcc tcgacctggg
      301 aaaagtcgaa gatgtaggga ttgccccaag ggtcgaggat ttcattctca cgcagatact
      361 tgcctcgaat catggcatcc agtagattct tcggagcctt ccaactgttg gcttgcagat
      421 atctttgaac gactccgtgg atgcgctcaa agtctttctg gtatcgctgc aggagtgctt
      481 cgcttgcctg ctgctgtttt tcctcaaagg tgttgaccct gatcgggggc tccggaatgc
      541 ttggtgcaga cgccagaagc actcgggctg cctgctgctg gtcttcattc caacgcatcg
      601 ggacaatggg tccgggctgc ggaggcagtc tgacgagatc gtccagttca tagccatgaa
      661 tttcatagcg aggcttcatg atctcctgct ccagggtgaa ataaactttc tccaaccctg
      721 gatgaatttc ctgaagggcg aataccgcct cgtccacgat ggatattccc aacgctgagg
      781 taatcggatt ccctttgtca tctgtcacac ggaaatcaat gttggcggac tgccctggct
      841 tgtaagtctc tgtgtcgagt gtgatgccga tattcagagc gtccgcggga ttggcgtaga
      901 tcagccgggt atcgcgaacc aaatcggttc cgggcgtgaa gatatacgcc tggagtgcca
      961 gacttccaga catcgaagcg tcaatgtcaa ctgcgagcaa tgctctgccg gactgcaatt
     1021 caccggcata ggtcatcact gtctgacctt cacgaatcat atcgaggtag aacgtcccct
     1081 gtttcttggt tgctatcact tctgccttta tggtctctcc gacttggtag agtgctttat
     1141 cggttctgag gatgaggctc tccgcagtgg ctttggagtc aagcgcgaac tccttggttg
     1201 ccgttctgcc ctctttgtct tttgctcgaa tggtcagatt cgtgacttct gaaaccttgg
     1261 gcgtcaccga aagcacagcg atgccaacgt catttgtgtt ctgtttcctg gtcctatcgt
     1321 ccacagcgcc gtactccacg gtcacctcag cctctttgca cggactgccg tcgggatagg
     1381 agaccagaag gtagacgttg tttttcagat tggggaccaa actaccactt tccggtatcg
     1441 cgaagagact cagaggtgcg tccgagacag gaaccatcgc cactttatct tcgcgatgat
     1501 cggcggtgtc cacgaccttc gcttcgagtc gaacggatgt tttcccctgc tccagagggg
     1561 tgccggcgaa gtattcaggc agtcttagtt cgaactccca atgtccttca tccgagagtg
     1621 ttccttcgac agtgtcaaac tactcgaact gtatttcaaa tttggacgcg accaccgtga
     1681 ctttgccgcc ggagacgggc tttccaaaaa ggtaatctgc ctgaacttcg cctttcagcg
     1741 tttcaccagg gagatagaag tcgcggtcgg ttttgacctg aaccttgaac ttcggcagca
     1801 cgtaccgttg cacgtcaacg ttcttctccg tctgacttga atccaggatc gccctcacga
     1861 tgtacgtgcc cagattcaac tcggttgcca gctgaaactc tgcggacgca attccaaaat
     1921 catcggtttc cgtcttcttc ttaaaaacct tgttgccctt cgagtccatg acctcgaaag
     1981 tgagtggttt tcccgcctgt ggagccagag ttccagctgc cagacaaagt gttcgcatgt
     2041 ggatcaattg tcccggttgg tatagcggtt tgtctgttgt caggagaatc tttgcttttc
     2101 gctgaaccga aacgctctgg agtacctcgt cttttcccag acgctcatgc tcaacggtca
     2161 ctttcaactg gcaggtgcct tcgacatcag aaggcacgtc gaactgagcc atcgccgatc
     2221 cgtttttgcg tgtctttcct tcaaaaagtt tccgtgcatt ctccttctca atgagggaaa
     2281 tggtgactct ggcgtcattg accggccttc ccgtcgcatg attcagagcc accacccgga
     2341 gactggcttt tgttcccgcg agaagatcac gataggccac gatgcgcgtc tccagttgag
     2401 ccaacgatgc catcaggctt cgagaaccct cttccacgtg ttttcccatc ttcagcctgt
     2461 atctcaggac acacaaggga accttctcgt cttccacgtt tccacgcagg acgaccgaga
     2521 ccttttggtc aagcgatgcc aaatccaaca acgacttctc ctcaagaaga gtcttgtcat
     2581 ccagatccac cagagtgaac ttcagttccc cagtcctttc cgaaccttgg ggagtcttca
     2641 actggcaggc gatccgaaga cccgccttga cccgtgttac ctcaatggac tcccggatca
     2701 gccacccctc attgcgctcc tgcgccaaac caaccccgac catgagagtc cacaacccga
     2761 caatcccgag cacgacaatc agagagcgag acatcatgag tgaaacctcc gtcacaataa
     2821 gttttcagtc cgtatagacg aacgcccttg agaaaagttc taccgtcatc ttggttacct
     2881 gaaaacaatt ctagtgtacc atctcgttca acgcaactgg gatgattgac gaggacgtgc
     2941 accagcggat cgagtctctt cacttcggga cggatgagaa gagtagataa acgacattag
     3001 atcaaataga taggttgaca tctggctctt tagtattcgg attggcacgc cttctgctat
     3061 ggaaggaacc agttgcatgc aattgtcgga gccacgagga aagtggcagt acagcgagga
     3121 acaaagaaag gaacgatagc aatgaaaaag atgatgattc tggccacgtt gacagtcatg
     3181 ctgatgcctt ttgcggcacg agcacaggag atgcagtttc gccttccgag tcccgaggtt
     3241 ttggagaaac tcgatttgag tcaggagcag aaggatcagc tgaaagacat tcattccaag
     3301 gttggcgatc aggtgaagca gctgcaggag aaatgccgcg aacaggccaa cgagatcaag
     3361 gaagccctcg gtgccgccga accgaacaag aaggccattt caaaggcggc caagcagctc
     3421 ggtgagactc gttcaaagat gcttgaactg caagctgaga gccttgcgga tgctcttgtc
     3481 ctgctgacac cgcaacagcg tgagcaggtt aaggaatggg tgaagagcca caagggagca
     3541 gggctgaccc gcggtttcgg tgcgcgagaa ggcattgggc aaggcagagc gtctgagccc
     3601 aacagaagag aaggactccg ccagcgacag ggttttggcg atgacagggg aagaggtttc
     3661 cgtggcgaac aagggatgag gcctgagatc agagagagga ttcgcgagag gcttcaagat
     3721 atggagccag agaaacgtgc tcagatgctt cgacggttcc gtcagaattg gtctcctgag
     3781 ggggaagaag ggagaggctc tggtggacag cgaatgcgtg accgacagcg tgatcaggac
     3841 tgtcctcccg agtccgagcc gaaagaagac tgagagttct ttcaccaaca agtcattcca
     3901 gaccgacctg ggtttacagt acaggtcatc gagacagggt gaagagcgca ccgcaaagca
     3961 tcggatggtt cagatctgag cgagtgcgct cttcgctttt tctcaaacgc tcttttttct
     4021 tgacgttatg cggatgtttg atatatttca acttagtcat tgccagttca tattgtttgg
     4081 aaggacaaac aggctggcac gggtaggatt ctgtgtggag tttgcacttt ctggactgat
     4141 cgattattgt gctcatgact actattcagg cgtcgcattc ttcgggaggc aatctgaagt
     4201 gatgaaaagc aagcaagaga ctgtaaccgt cagaagattg ttcgctggag tcgtctttct
     4261 ttgtttcctg ggaatggcat cgggcaatct gatggctgct gagttgctcg gcgtaagggt
     4321 ctacggaaag gtggtcaact cagcaggtta tgtgataatc gctgacgcaa cggtcagtct
     4381 cgtcagcagc aaccgcgtgt atgtgacaac cagcgatgct gaaggagact atgagtttac
     4441 cgacgttgaa gtgggtttct atcaaatcag cgcaacaaaa gaaggctttc tgcctcagac
     4501 cgctgaattg gatgtggacg gcagcaaacc ggagtttctt aaaaacttct ttctggaact
     4561 tgaaagatcg ggagatctta acaatgacaa gatcatcagt gcagaggatc tgatggtgtt
     4621 tctgaaacac tggtacgcgg aaagtcctca agccagttac aactacctcg cagacttcaa
     4681 ctatgatcgc atcattgacg agagcgatct gctcattttg cttgcccggt ggcatcagca
     4741 aacaggcggc aactagtctc gattctccat gatgattttc ccgaacgctc tccagtattc
     4801 ttggcaggca tcatcgcatc ttgatacact tctcacttga cctgaattga aaggctcgta
     4861 tattcgtctg taaaaccaag actgggaaga ggtatctttg gcctgcaaga aagccctgtc
     4921 tgaggatgga gaaccatgga aacggattgt gcgcaaatgc aagagcgtct gagtaacctt
     4981 cgtcgaacaa ttcacaaatg tcctgaactg ggatttcagg agatcgaaac ggggaagttg
     5041 atagcaaaga ccttgagtga tctaggtctc gaagttcaga ccaatgtcaa caagactggt
     5101 gtcgttggag tcttgaaggg agccaagcca ggtcgcacca ttgcgtttcg cgcagacatg
     5161 gatgctctgc cgattcaaga gttgaatgag actccctaca aatcacagaa acccaatgtg
     5221 atgcatgcct gtggtcacga cggcaacacg accattctgc tgggaactgc catgttgttg
     5281 tgccaacaga aagacagttt gtgcggaaac gtcaaattca ttttccagcc ttccgaagag
     5341 gctcctccag gtggtgcaaa aggcatgatc gaagagggtg tattggagaa tccgaaagtg
     5401 gatgccgttg tgggagtcca cgttgacact gaccatccaa ttggcaaagc gtctgtccga
     5461 ttcggagcac acacggcaaa cgccgatcgt atccatatag ttatcaaggg acgtggcggc
     5521 cacgcggcac aaccgcacaa atgcgtggat ccgatctaca ttagtgcgat ggtgatccaa
     5581 gccatccatg ggcttccgag cagatttgtg gatcccgttg agccctttgt tgtgacactt
     5641 gggaaaatct ctgggggaga taccttcaac gttattccca atgacgtgga gattcttgga
     5701 actgtccgca ccgtcacgac tgagacacaa gaccgagtgc tcgaacagat tcggctgatc
     5761 gttgagacca ttcccaagac ctacaatggc gagggaacca tcgaagtcag gagaggctat
     5821 ccggtctgta tcaacgatgc tggcatctcc agtttgatcc aagagtgttg tgaggcagaa
     5881 cttggagagg atacggtgag cgttggaacg actccgtcca tggggggcga ggactttgcg
     5941 tatttcgcgc agaaagtccc gtcgtctgtt cttatggttg gtgtccgcaa cgaggaactt
     6001 ggcatggtct acccctggca tcatgctcgg ttcgacatgg atgagagagg actcgtcatc
     6061 ggtgcgaaat gtcttgctcg aattgcgaca accttcctct gtcgcgactg aacggctctg
     6121 aggagaacac atgattaccg tgaagaacac ttccatcaaa cgtgcgctct gggcttttcc
     6181 tttgctgctg atcttctggc tcatctcgac tgctcgcgct gtggaacctg ccgcgcacgt
     6241 ttcttccgcg acggatgaga aagcgcagaa actcgtcaag aaagtctctg ctttggaaga
     6301 caagatcgat cttgattacg cggagaagaa gaaacaggaa attcttacca aatatcccac
     6361 cttggagaca gactacctca attttgttcg aagcaagtat ccgacacttg aaacggagat
     6421 tatcgagatt ttcaagaagt accccaaacc tttgaaacaa tatggaactc gcaacgacac
     6481 aggcaaaacc aaggtcacct tcgaactcat cgagaagatg tgtatggcgg acggcaagtg
     6541 ttcggatgaa cttggcgatc tgctttcgaa aaagtatccg aatttcgcca aggattccgc
//
LOCUS       DAOQUC010000026         6597 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_12820_length_6597_cov_3.511159, whole genome shotgun sequence.
ACCESSION   DAOQUC010000026 DAOQUC010000000
VERSION     DAOQUC010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6597
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12820_length_6597_cov_3.511159"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..766)
                     /locus_tag="PKH07_00850"
     CDS             complement(<1..766)
                     /locus_tag="PKH07_00850"
                     /inference="COORDINATES: protein motif:HMM:NF013662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit F"
                     /protein_id="HNT33528.1"
                     /translation="MSNAARVTVDLEQMKQSGMAKIYPDRVKVSVGMATCGLATGAGK
                     VYDALKRKIEETGSDIVLSSSGCLGFCEMEPLVCVQRPGKPKVVYSNLTVKQVDDFFE
                     ALVDGKDKEDWVLCRMDGDEIIVEDSCKALGEDAAAGRYTPYEQLPFFSKQLKIVLRN
                     CGFIDPEDIEEYIARGGYSALKKVLTTMTPEQVIEEIKKSGLRGRGGAGFPTGLKWEF
                     CRKASGDEKYIICNADEGDPGAYMDRSVLEGDPHSVI"
     gene            complement(759..1151)
                     /locus_tag="PKH07_00855"
     CDS             complement(759..1151)
                     /locus_tag="PKH07_00855"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HNT33529.1"
                     /translation="MPVLKDINRTYNYLPQNILRYVSQEMSIPLSLIYRIVTFYSAFS
                     LVPRGRHTINICMGTTCYVRGAGKLLSALEDQLKVAAGGTTEDRRFSLETVRCLGCCS
                     LAPVITVDGETYGKVDQNKVLEILEQYE"
     gene            complement(1266..1601)
                     /locus_tag="PKH07_00860"
                     /pseudo
     CDS             complement(1266..1601)
                     /locus_tag="PKH07_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177460.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
     gene            complement(1795..2682)
                     /locus_tag="PKH07_00865"
     CDS             complement(1795..2682)
                     /locus_tag="PKH07_00865"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit B family protein"
                     /protein_id="HNT33530.1"
                     /translation="MKSAFFLGCTIPVRAQNFEMATREVAKALGIELVDLPSAGCCGY
                     PVRSVDAKRARLVAAAVLADAEAVSDSLLTICTACTGVLTETAHELSEDAELLTEVNE
                     ELAKVGKQYRGTVKVRHFARVLFEDVPPETITNKITVDLSNLRFACHYGCHYLKPHEA
                     YKEFDSVENPQSLDKLVQLTGAQTVSYLGKKRCCGGACLAVDENLALAITKEKLDTIK
                     KQKADGIVLICPFCSIMYDTNQKKIEKDFESVYSIPVLFYPQLLGLAFGMDPKNLGFQ
                     MNRVKTKELLDRIAQPVSS"
     gene            complement(2679..3152)
                     /locus_tag="PKH07_00870"
     CDS             complement(2679..3152)
                     /locus_tag="PKH07_00870"
                     /inference="COORDINATES: protein motif:HMM:NF024581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNT33531.1"
                     /translation="MPETLNRSSEFLRRVMATETGKKLARCFACGTCSSGCPVHAQKP
                     EFDPRKIIRMTLLGLEDNLLKSKEIWMCSTCYTCRERCPQEVGCAEIIIELRNIAAEM
                     GYTPEAFKMQMEALRKFGRVYEIEDFDNKKRAKMGLSELETKARIEPLIPEKEER"
     gene            complement(3251..4411)
                     /locus_tag="PKH07_00875"
     CDS             complement(3251..4411)
                     /locus_tag="PKH07_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HNT33532.1"
                     /translation="MKLFVILGVFLMAVDAYPSDNLLLPDPLKSAEGMVISSSEQWQA
                     VRRAEILELFRTHVYGRTPVGRPTDLRFETKEITPTALSGSATLKLVNIRYSGAGGEG
                     MIELSVFIPNAVPKPVPGFLLICNRSRENIDHTRENKSPFWPVEWIIERGYVAAAFLN
                     ADVDPDKDDGFQDGVHGIFDSKNVPRSPDAWGTIAAWAWGASRVMDYLETDEDVDATR
                     MAAIGHSRGGKTALWCGAEDQRFSMAISNNSGCTGAAIARRKQGESIEAINRRFPHWF
                     CANYKEYNDKEDELPVDQHELVALMAPRLVYVASATEDNWADPMGEFLACVHATPVYS
                     LFGLTGLNTDEMPKPEHPLHDGHIGYHLRTGGHGLTEYDWNCYMDFADKHWK"
     gene            4644..5126
                     /locus_tag="PKH07_00880"
     CDS             4644..5126
                     /locus_tag="PKH07_00880"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNT33533.1"
                     /translation="MTLAKELGYLKPIASREHEAVLNVLMTAGMMTREGDRILRPFGM
                     TGAQLNVLILLGYQAHQGCMTQSDLADKLLVNRSNVTGLVDRMQRAGWVERIPDPQDR
                     RVNLLRLTTKGRGILEKVEPVYFERIHSVMNCITPNEQKELCTMLERVRAEIRGGLEQ
                     "
     gene            5240..>6597
                     /locus_tag="PKH07_00885"
     CDS             5240..>6597
                     /locus_tag="PKH07_00885"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNT33534.1"
                     /translation="MKLLLIAPASGKWRHVGRSRWFSGKTFRFSLLSLLSVAAETPKD
                     VQVRLVDEQVEDIPWNESFDLVGITCMTALAPRAYEIADRFRVLGTPVVLGGMHPTFL
                     PEEALLHADAVVVGEAEGIWEGVIADARAGRMHGIYQGGVDRGLAGLKHPPHHLLKGK
                     HYATVHAVQATRGCPHQCVFCTVSAFNHGTQRQRPVDEVVQEVSEIPHRFFIFVDDNL
                     TADREYAMSLFRALQPLNKLWVTQSTLGIARDKEMIVLAAKAGCVGLFVGLETFTEKN
                     LTSVNKTCHQVEEYQHCVSELHRHGIGVEAGIVLGMGYDTPGTFEKTLEVLEQLQIDA
                     IQVSIFTPVPGTPQYQTMQSKITDHDWAHYDFHHVVFQPEGLSAEALQAGHDWLTQEF
                     YKPWRILRRLVRHMTRPRALSGLPFVASVNFAYLGRVLRWGIKGFNPARVAEKPFPVT
                     KRR"
BASE COUNT         1610 a         1818 c         1624 g         1545 t
ORIGIN      
        1 cgatgactga gtgcggatct ccttcgagaa cacttcgatc catgtaggca ccgggatctc
       61 cttcatcggc attgcagatg atgtatttct cgtcaccgga tgccttacgg cagaactccc
      121 atttcaaacc cgttgggaat ccagcaccac cacgtccacg aagaccggat ttcttgatct
      181 cctcgatgac ctgttcgggc gtcatcgtag tcagaacctt cttcaacgca gaatacccac
      241 cccgggcgat gtactcctca atgtcttcag gatcaataaa gccacagttg cgcaagacga
      301 tcttcaactg cttgctgaag aaaggcagct gctcataggg ggtatatctc cctgcggcgg
      361 cgtcttcacc aagcgccttg caggaatcct cgacgatgat ctcatcaccg tccatacggc
      421 acaacaccca gtcttctttg tctttaccgt caacaagagc ctcaaaaaaa tcatccacct
      481 gtttgacggt caaattggag tagacaactt tcggtttccc gggacgttga acgcagacaa
      541 gcggctccat ctcacagaat ccaagacatc cggatgagga aagaacaata tccgaaccgg
      601 tctcctcaat cttacgcttc agagcgtcat agactttccc cgcacctgtt gccagaccac
      661 aggtcgccat accgacggag acctttactc ggtcgggata gatcttcgcc atcccagact
      721 gcttcatctg ctcgagatca actgtgactc ttgctgcatt actcatactg ctcgagaatc
      781 tccaggactt tgttctgatc caccttgccg taagtctcac catcaacggt gatgaccggc
      841 gccaaactgc aacaacccaa acaacgaacc gtctccagac tgaagcggcg atcttctgtt
      901 gtaccgcccg ccgcaacctt gagctgatct tccaaggcag acagaagttt tccggcacct
      961 cgaacgtagc aggtcgtccc catacagata ttgattgtgt gacgaccgcg aggcacgaga
     1021 ctgaacgcac tgtaaaaagt gacgatccga tagatcagac tcagggggat actcatttcc
     1081 tgcgagacat acctgagaat gttctgtgga agatagttat aggtgcggtt gatgtccttc
     1141 aaaacaggca tcaagggacc cttcttgttt cgatgcgctt ggatgatcgg atcaaccttc
     1201 gccttgtctt cttccgagat ttccactgag acaactcgtg tcggttccgt gtagatctca
     1261 tatttgaccg gacagttctg ggtgcagaca ccgcagccag tgcacttcga accgtcaacg
     1321 aaacgggcat tccttctcac gcgaaccttg aggtttcccg gatccccatc aatgccccga
     1381 acctctccga gcgtgatgat cgaaatgttg ggatgacgtc ccgcttcaac aagttttggg
     1441 gacaggatac acatggagca gtcgttggtc ggaaaagtct tgtccaactg tgccatgaca
     1501 ccgccgatgc tcggtttctc ctcgaccatg tagacctgat agcccgagtc cgctaggtca
     1561 agcgccgctt gcacaccgcc aatgccccca ccaacgacca taacgcttcc cacggtggtt
     1621 gctgttttga tctgatccgt ctgcgtcaac gtgtgcaagc cctgggcaga gagaccaaca
     1681 ccagagccag cacattcgca ccgactcgag caggcatcct tcgagtccac attcccttgc
     1741 gtacaagtgt ccaatttcct tgtctccaca attcagatct gacccagttt ggggtcagga
     1801 acttacaggc tgtgcaatcc gatcaagcaa ctccttggtc ttcacacgat tcatctgaaa
     1861 acccaaattc tttggatcca tgccaaacgc caaaccgaga agctggggat agaaaagcac
     1921 gggaattgag tacaccgact caaagtcctt ctcgatcttc ttctggttgg tgtcatacat
     1981 gatgctgcaa aacggacaga tcaggacgat tccgtcggcc ttctgtttct tgatggtatc
     2041 cagtttttcc ttggtgatgg caagtgcaag gttctcatcc acagcgagac aggctccccc
     2101 gcagcagcgc tttttgccaa ggtaagaaac ggtctgggca ccagtcaact gcacaagttt
     2161 gtcgagactc tgtggatttt caacagagtc gaattccttg taagcttcat gaggcttcag
     2221 atagtgacat ccatagtgac aggcaaaacg caggttggag agatcaacgg tgatcttgtt
     2281 ggtgatggtc tcgggtggca cgtcttcgaa aagtacccgc gcaaagtgcc gaaccttcac
     2341 ggtacctcgg tactgcttgc cgaccttcgc caattcctcg ttgacctctg taagcaactc
     2401 ggcgtcttcc gaaagctcat gggcggtctc cgttaataca ccggtacagg cggtacagat
     2461 ggtcagaagg gagtccgaca ctgcttccgc atctgcaaga accgcagcag caacaagccg
     2521 agccctcttg gcgtctacgg agcggacggg atagccacag cagccggcag acggcaaatc
     2581 aaccaactca atacccaacg ctttggcaac ttctctcgtt gccatctcaa aattctgcgc
     2641 ccgaacgggg atggtacacc caagaaagaa cgccgatttc atcgttcctc cttctcaggg
     2701 atcagaggtt cgattcgggc tttggtctcc agttcagaca agcccatctt cgcccttttc
     2761 ttgttgtcaa aatcctcgat ctcatagacc ctgccaaact tgcgaagcgc ttccatctgc
     2821 atcttgaacg cctcaggagt gtatcccatc tcagcggcta tgtttctgag ttcgatgatg
     2881 atctcagcac acccaacctc ttgaggacac ctttcccggc aggtgtaaca ggtgctgcac
     2941 atccatatct ctttagattt taacaggttg tcttccaaac ccagcagcgt catccgaatg
     3001 atcttcctgg gatcgaattc aggcttctgc gcatggacag ggcaaccgga agaacaggta
     3061 ccacaggcga aacaccgagc cagtttcttg cccgtttccg ttgccatgac gcggcgaagg
     3121 aactcactgc ttctgttcag ggtctcaggc atccagcatt cctttccttc ctgaaatcta
     3181 aacggtttca gacactaatt gttatgcttt cttttgtcaa caatctgcgg ttcccaagac
     3241 agggttcaga tcatttccag tgtttgtccg caaaatccat atagcaattc cagtcgtact
     3301 cggtcagccc atgtccaccc gttcgaagat ggtagccgat atgaccatcg tgcagaggat
     3361 gttccggctt tggcatctca tcggtattca gacctgtcag cccaaacaga ctgtacacgg
     3421 gcgtcgcatg gacacaagcc aaaaactcac ccattggatc cgcccagttg tcctcggttg
     3481 cgcttgcaac gtagaccaac cgaggtgcca tcaatgcaac cagttcatgc tgatccacag
     3541 ggagttcatc ttctttgtca ttgtattctt tatagttagc acagaaccag tgtggaaagc
     3601 gtcggttgat ggcttcgatg ctctccccct gtttccggcg agcaatggct gcaccagtgc
     3661 agccggaatt gttgctgatt gccatggaga aacgctgatc ctcagcacca caccagagtg
     3721 cagttttacc accgcgggaa tgcccgatgg ccgccatccg tgtggcatca acatcctcat
     3781 cggtctcaag atagtccatc acacggctgg ctccccacgc ccatgcagcg atggtacccc
     3841 aggcatcggg tgaccttggc acattcttgg agtcgaatat cccatgaaca ccatcctgaa
     3901 atccatcatc cttgtcaggg tctacatccg cattcaggaa cgcagccgcc acatacccgc
     3961 gctcaatgat ccactccaca ggccaaaacg ggcttttgtt ctctcgcgta tggtcaatgt
     4021 tctctctgga acggttgcag atcaaaagaa acccaggaac cggcttggga acggcatttg
     4081 gaatgaacac agaaagttct atcatcccct ctccaccggc tccggagtat ctgatgttga
     4141 cgagtttgag cgttgcggaa cctgacagtg ctgttggtgt gatttccttt gtctcaaatc
     4201 ggagatcggt aggccttccg actggtgttc ggccatagac atgcgtccgg aagagttcga
     4261 gaatctctgc gcggcgaacg gcttgccatt gctctgacga tgatatcacc atcccctctg
     4321 cacttttcaa cgggtcggga agcagcaggt tgtctgaagg atatgcgtca acagccataa
     4381 gaaacactcc aagaatcaca aacagtttca caagtcttcc tcttcatcct caggcggttg
     4441 agtcactcca gcccgcctga cggtctccgt cttgttgaca agagagacac acgatatttc
     4501 cccgccaaga gtacaaaaaa acctatcaga aaacaacgaa cacaaagaag agaaaatttc
     4561 tcttgacatt tatagtttat acgttaacaa tctacataga cacaaataaa tgaagagact
     4621 cacttcaggc aaggagcgct cgcatgacac tggcaaaaga acttggatat ctgaagccga
     4681 tcgcgtccag agaacatgaa gcagttctga acgtcctgat gactgctggc atgatgacgc
     4741 gggaaggtga tcgaatcctt cggcctttcg gcatgaccgg tgcacagttg aacgtcctca
     4801 tcctgctcgg gtaccaggca caccagggtt gtatgaccca aagcgacctc gcggacaaac
     4861 tgctggtgaa ccgttccaat gtcactggac ttgtggatcg aatgcagcgt gccggatggg
     4921 tggagaggat cccagatcct caagaccgtc gggtgaatct tcttcgccta acaaccaagg
     4981 gaagagggat tctcgagaag gtcgagccgg tctacttcga gagaatccat agtgtgatga
     5041 actgcatcac cccgaatgag cagaaagaac tgtgcaccat gttggagcga gtacgggctg
     5101 agattcgcgg cggtctggaa caatagagga ggaaaacagg gcaccacagc agatgtgccc
     5161 tttcttttca gactatagtt catatataaa caatatagga ctatacagat agaagcgagt
     5221 gttcgaggag gtcaaaatca tgaaacttct gttgattgcg ccagcgagtg ggaaatggag
     5281 acacgttgga aggtcgagat ggttcagtgg aaagacattt cgtttctctc tcttgagcct
     5341 gttgagcgtt gcggcagaga caccaaaaga cgtccaagtc cgtctggtgg acgaacaggt
     5401 tgaggacatc ccttggaacg agtcctttga tctggttggc attacctgca tgacagcgtt
     5461 ggctccaagg gcctatgaga ttgcagatcg gtttcgcgtt cttggtacgc cagttgtgct
     5521 cggtggcatg cacccaacat ttctaccgga agaagcgttg cttcacgccg acgctgttgt
     5581 tgtcggcgag gcggaaggaa tctgggaggg ggtcatagcc gatgcacggg caggtcgaat
     5641 gcacggcatc taccagggag gggttgatcg tggtctggca ggcctcaagc atccaccgca
     5701 ccatctcctt aaaggcaagc actatgcgac agttcatgcg gtgcaggcaa cgcgaggctg
     5761 cccgcatcag tgcgtgttct gcacagtgtc ggcattcaac cacggaacac agcgccaaag
     5821 acctgtggat gaggttgtgc aggaagtctc ggagataccg caccgtttct tcatcttcgt
     5881 ggatgacaac ctgaccgcag acagagagta tgccatgagt ctctttcgtg cgctgcagcc
     5941 gctcaacaaa ctctgggtca ctcagtccac gctcggaatc gccagggaca aggagatgat
     6001 cgtactggcg gcaaaggcgg gatgtgttgg cctttttgtc ggtctggaga cctttacaga
     6061 gaagaatctg acttcagtaa acaagacctg ccatcaggtt gaggagtatc agcactgcgt
     6121 atccgaactc catcgtcacg ggatcggtgt cgaggcaggt atcgttctcg gaatgggcta
     6181 cgatactccg ggcacattcg agaaaacact tgaggtgctt gagcaactcc aaattgatgc
     6241 cattcaggtc tccatcttta cacccgttcc aggaacgcca cagtaccaga ccatgcagtc
     6301 caaaattact gaccatgact gggcacacta tgactttcat cacgttgttt ttcagcctga
     6361 aggcctgtcc gcagaagcac ttcaggcggg acatgactgg ctgacgcaag agttttacaa
     6421 gccatggaga attctccgac gccttgttcg ccacatgacc agaccccgcg ctctgagtgg
     6481 cttgcccttt gttgcctcgg tcaattttgc ctatcttgga cgagtgctcc gatggggaat
     6541 caaaggattc aatccggcac gagttgccga aaaaccattt ccggtaacga aacgccg
//
LOCUS       DAOQUC010000027         6526 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_13004_length_6526_cov_4.138464, whole genome shotgun sequence.
ACCESSION   DAOQUC010000027 DAOQUC010000000
VERSION     DAOQUC010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6526
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13004_length_6526_cov_4.138464"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(201..449)
                     /locus_tag="PKH07_00890"
     CDS             complement(201..449)
                     /locus_tag="PKH07_00890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33535.1"
                     /translation="MRLLFVQPSQGDFVRQRIFQPGIEAPLNVACLAAYMEQKGVTNS
                     ILDMRALHHPEAALVKVLQEEKPHIVGISAFTAEARNA"
     gene            complement(446..1270)
                     /locus_tag="PKH07_00895"
     CDS             complement(446..1270)
                     /locus_tag="PKH07_00895"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNT33536.1"
                     /translation="MTDLSIVIVSFNGRELLQECLSSLFAAKLSLSYEVHVVDNASSD
                     GTPNMVQNEFPQVHLVANADNIGFARANNQILRDLKARYALLLNPDMVVAQNAIEAMV
                     GFMDTHADIGALGPRLLNPDGSLQYSCRRFPNAVAIFLRGLGLFRMLPKLPMRQRYFM
                     LDWDHRTVAEVDWIMGSCMMLRREALQQVGFLDERFFMYYEDVDLCRRMWKQWKVCYL
                     PEIPMLHHHRQESHRLSSLRVRFIHLQSAWRFFAKHGWCPTRPECLRVRVPSETIQ"
     gene            complement(1284..2783)
                     /locus_tag="PKH07_00900"
     CDS             complement(1284..2783)
                     /locus_tag="PKH07_00900"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNT33537.1"
                     /translation="MKITFVYPDILEIVPDYKGAFYMGIAILSAVLKREGHETSLIHI
                     TDPEYDLPRFEADLSVHQPNLIAFSSTTNGWERVRHLAGFLASRGSTIPTIYGGIHPT
                     LAPQECIAAPGIDMIAIGESEQPLANLCRALQQGQEFDKIPNLWVKKGSTVIRNPLSP
                     LLQNLDDLPFADRALFDYPNLHFELQGAATIMASRGCPFACTYCCNTALRRLYNDLGS
                     YVRFRSVRNVIDEIKQVRRDYAFVRRVHFDDDILFLRREWAEEFADLYVREVRLPFCC
                     NVHPSLCTAQNVETLARAGCDELRIGIESGNEMIRRTVLGRSLDEGKILEAFALARSK
                     GMRTLSFNMVGIPDETPSCILESVKLNARAGVDEIQLTAFHPYPGSALYEVCLEKGMI
                     SKRRVTSYYVDSILDLPDLSREQLRMFQRYFCTFSRVYRKVFRLPTPVRKAAEWLLDG
                     ILTSRMAPSLLRGIRPIAHRVGVVLRVLMRRNRSKLKCVNQERPGLKEP"
     gene            complement(2785..3966)
                     /locus_tag="PKH07_00905"
     CDS             complement(2785..3966)
                     /locus_tag="PKH07_00905"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNT33538.1"
                     /translation="MQQKKRINVLFVSPGYKPYIGGTERIIEQIGNEFLQSGHVARVG
                     VLTTFMDFNVSPPNENRNLPSRETIDGIEVFRLHFSPRRLRLFYTLPAGLFSTKVRQV
                     IRDFQPDVIHFLLNEWYVANLWIYWLTRKCSKHVMLIPFHEPPDNFHFLLMKWIISFL
                     GRRVDQVHVSCSHIKDRAAEYYGIPKDKIEVIPLGLTSNSRSQGQGGNRSGEPLTLIS
                     VGRLALEKGQLELVRLFHQVRERFNKQVRLVLVGGDGGHREAIEEFIRQNQLEDSVHI
                     AGFVSQESLEALYRESDIFILLTRNESFCLAFAEALSYGIPCIGYAIGALETSFTQGV
                     VLVEPFDSNAVANRLQELVNDDALRMRLGQEAHEFAWKAFSWKTTCERLLRLYQGANK
                     D"
     gene            complement(3973..5412)
                     /locus_tag="PKH07_00910"
     CDS             complement(3973..5412)
                     /locus_tag="PKH07_00910"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HNT33539.1"
                     /translation="MSAIGRIIRNTSWQIVGQAVHLVLSLALLAVIPRYLGAEEYGKY
                     SLIFVIIALFATLTDFGTNDIVVRELSKDRSRIAQAVIELLILKTVLGLAVIAISIAA
                     VAILGYAEDLRRCFALASISLLFLSLSSTGNIVFRVNLSMQRGVFATAVKDILALLLS
                     LLVIFFDGRLIHFILVVVVANFVNLIVVLALVREFLHAPSWSLDLDFWKRILRSSLPL
                     GCAYSVVSLYMTVDMLILEHIRGAEEVGYYNASYKFVYLSVVFPLALATSLFPFMSEY
                     SKINQEKLKTLFQKSFDYTLLVALPLATSLTVLAPKIIVLVFGSEFTPSIPALQILVW
                     GVALMFPGIVFGYLMIALDQQKKSLVIDCWGLGVNVGLNLVLIPMIGFIGAAIVTVVT
                     EFCVLAPTVYLIQRNLKYRLCWNKGMRSLLAAAVCGLTLAVAARCPLLIQGIIGGLVL
                     LVAVWLLRLVSNEDIELVLQRPRPRSAQG"
     gene            complement(5439..>6526)
                     /locus_tag="PKH07_00915"
     CDS             complement(5439..>6526)
                     /locus_tag="PKH07_00915"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HNT33540.1"
                     /translation="WLWGLLLCIMVLGRLLLRNMLETLRRNGMNRRFVLIAGSRSNAQ
                     QFAEKIERRPELGYVLVGFVDDAPEPSLLKGQFQSRLIGTLKDIPQVLRNSVVDEVII
                     SLPIKSYYEQIEGIVRLCAEHGIIVRFLRGVFSLTSANFRFSLLDDMPVITLCSSPYE
                     DPQILVKRLFDIVVSAFLLVLTLPLLLAVYVTLKVLSPGPAFFIQKRVGYNKRIFNLV
                     KFRTMVEDAEALISSLEHLNETDGPTFKMKNDPRLTGIGRILRSTSIDELPQLWNVFK
                     GDMSLVGPRPLPVRDYAGFDSEWQSKRFSMKPGITCFWQVGGRSKIDFERWMEMDVKY
                     VDTWSLWLDIKILLKTIPTVLLRRGAY"
BASE COUNT         1678 a         1731 c         1655 g         1462 t
ORIGIN      
        1 atcccctgga catctgatac atcttgacag ccgtcgatcc tttcgaccaa ttccgccaga
       61 gtcagttccc cttctccgtg gacaatgtaa tcaacatccg gtgtctcgcg aagaaatgac
      121 tcgggaagtg aggaagcatg gtgaccgccg agaatgacca cagcttcctt gttgatagat
      181 ttgaccgttc gagcaacttt ttatgcattt cgcgcttcag cggtaaacgc gctgatgccg
      241 acaatgtgag gtttttcctc ttgaaggact ttcacaagag ccgcttctgg atgatgcaat
      301 gccctcatgt ccagaatgga attcgtcaca cccttttgtt ccatatatgc agccaggcag
      361 gccacgttca gaggtgcctc aatccccggt tggaaaatac gctgtcggac aaaatcaccc
      421 tgactaggct gcacaaatag aagtctcatt gtatcgtctc cgagggcact ctgacgcgca
      481 agcactctgg ccttgtgggg caccagccat gctttgcgaa aaatcgccag gcactctgta
      541 agtgaatgaa ccgtacacga agggaagaca gacgatggct ctcctggcga tgatggtgaa
      601 gcatcgggat ctctggaaga tagcagacct tccactgctt ccacattctc cggcacaagt
      661 ccacgtcctc atagtacatg aagaaccgct cgtcaagaaa ccccacctgc tgcagggctt
      721 cccggcgaag catcatgcag gaacccatga tccagtcaac ttccgcaacc gtgcgatgat
      781 cccagtcgag catgaagtac cgttgcctca tcggcagttt cggcagcatc cggaatagac
      841 ccagtccgcg gagaaagatg gccaccgcat ttggaaaccg cctgcaggag tactgtagag
      901 aaccgtcagg gttgagcagt cttgggccca gagcgccgat gtctgcatga gtatccataa
      961 aaccaaccat ggcttcgatt gcattctgag caaccaccat atccggattc agcagtaacg
     1021 cgtagcgtgc tttcagatca cggaggattt gattgttggc tcttgcaaaa ccgatgttgt
     1081 cggcattggc gacgaggtga acttgtggga actcgttctg aaccatattc ggagtgccat
     1141 ccgatgaagc attgtccaca acatggactt cataggacag actcagtttc gccgcaaaca
     1201 gtgaggaaag gcattcctgc agcagctctc tgccgttgaa actgacgatg acaatgctga
     1261 gatcagtcaa tgctgcaatt cctctaaggt tctttcagtc caggtctttc ctgattgaca
     1321 catttcaact tgcttcggtt gcgacgcatc aagaccctga gcacaacgcc cactcgatgc
     1381 gcaatcggtc ggatccctct cagcagactt ggagccatgc ggctggtcag aatgccgtcc
     1441 agaagccatt cagctgcctt cctcacggga gttggcaatc ggaacacttt ccggtagacc
     1501 cgactgaaag tgcagaaata cctctggaac atccgcaact gctctcgact gagatcaggc
     1561 aagtccagaa tgctgtccac ataataactg gtcacacggc gtttgctgat catgcccttt
     1621 tccaggcaga cttcgtagag tgcggagcct ggatacgggt gaaaagcggt cagttggatc
     1681 tcgtctacgc ccgcacgggc gttcaatttg acactctcca ggatacacga tggtgtctcg
     1741 tctggtatgc cgaccatgtt gaacgacagg gtgcgcatcc ctttgctcct ggccaacgca
     1801 aaggcctcca gaatcttccc ctcatccagc gatcgcccca ggacagtacg ccgaatcatc
     1861 tcattgccac tctcgatgcc aatcctgagt tcgtcacagc ctgctctggc gagtgtctcg
     1921 acattctgtg ctgtgcagag acttggatga acattgcagc agaagggaag tctgacttcg
     1981 cgcacataga ggtccgcaaa ctcctccgcc cattcgcgtc gcagaaacag aatatcgtcg
     2041 tcgaaatgga ctcgtcggac aaaggcgtag tcacgccgga cttgcttgat ttcatcaatg
     2101 acatttctga cagagcggaa acgaacatac gatcccaagt cattgtagag tcttcgcaac
     2161 gccgtgttac agcaataggt gcacgcaaac ggacaacccc gtgacgccat gatcgtggcc
     2221 gcaccctgaa gctcgaagtg cagattcgga tagtcgaaca aagctcggtc tgcaaatggg
     2281 agatcgtcca agttctgaag caggggactc aggggattgc ggatgaccgt ggaccccttc
     2341 ttgacccaaa ggttgggtat cttgtcgaat tcctgaccct gctgcaacgc tcgacacaga
     2401 tttgccagag gctgctctga ctcaccaatg gcgatcatat caatgccggg tgctgcgatg
     2461 cactcctgcg gggcgagcgt cgggtggatt cctccgtaga tcgttgggat tgtggaacca
     2521 cgggatgcca gaaagcccgc aagatgcctg actctctccc aaccgtttgt cgtcgaggaa
     2581 aatgcgatca ggttcggctg gtgcacagag agatcagcct caaatctcgg caagtcatat
     2641 tcgggatctg tgatgtgaat cagcgatgtc tcatgccctt cccgcttcag tacagcggac
     2701 aggatggcaa ttcccatata gaaggcacct ttgtagtcag gcacaatctc cagaatgtca
     2761 ggatagacaa aagttatctt cacttcagtc tttgtttgct ccttgataga gtcgcagcag
     2821 acgctcgcat gttgtcttcc acgagaaagc cttccaagcg aattcatgcg cttcttggcc
     2881 caaacgcata cgcagtgcgt cgtcatttac caactcttgg agtctgttcg caaccgcgtt
     2941 ggagtcaaac ggctcaacaa gcactactcc ctgcgtgaac gaagtctcca atgcaccaat
     3001 cgcatatcct atgcacggaa tcccatagga gagagcttcc gcgaaagcca gacagaagga
     3061 ttcattacgt gtcaacagta taaagatatc ggattcccga tagagtgctt caagggattc
     3121 ctgagatacg aagccagcaa tgtgtactga atcctcaagt tggttttgcc ggatgaattc
     3181 ctcaatcgct tcacgatgcc ctccatcacc acccacaagc acaagcctga cctgcttgtt
     3241 gaatctctct cgcacttggt ggaacagcct gacaagttcc aactgtcctt tctcaagagc
     3301 aaggcgacca acgcttatca gagtcagggg ctcacctgag cggtttccac cctgaccttg
     3361 tgatctggaa tttgaggtca ggcccagagg aatcacctct atcttgtcct tggggatgcc
     3421 atagtactca gcagcgcgat ccttgatatg agagcagctg acatgcactt gatccactct
     3481 tctgcccaga aaggatatga tccatttcat caacagaaag tgaaagttgt caggcggctc
     3541 atggaaagga atcaacatga catgcttgct gcactttcgg gtcagccaat agatccagag
     3601 attggcgaca taccattcat tgagcaggaa atggatcacg tccggctgaa aatcccgaat
     3661 cacttgtctg acctttgtag aaaacagccc tgcgggcaag gtgtagaaca ggcgaagtct
     3721 tcggggtgag aagtgcaggc gaaagacttc tattccatca atggtctctc tggagggaag
     3781 atttcggttt tcatttggtg gagagacatt gaagtccatg aaagttgtca gaacgccgac
     3841 tctcgctaca tgacctgact gaagaaactc gttgccaatc tgttcgataa tccgctcggt
     3901 tcctccgata tagggtttgt aacccgggct gacaaagaga acattgattc ttttcttctg
     3961 ttgcataaga aattagcctt gggccgaacg tggtctgggt cgctgcaata ccagttcaat
     4021 gtcttcgttg gataccaacc gcaacaacca gacggctacc aggagcacaa ggcctccgat
     4081 aatcccttga atcaaaagag gacaccgtgc cgcaacagcc aacgtcaggc cacaaaccgc
     4141 agcggcaaga agggatcgca tccctttgtt ccagcagagc ctgtacttca ggttacgctg
     4201 tatgagatag actgttggcg ctagtacgca gaactctgtc acgacggtga ctatggcagc
     4261 tccaatgaaa ccaatcatcg ggatcaggac taggttcagc ccaacattga ctccaagacc
     4321 ccaacagtcg atcaccagcg actttttctg ctgatccaaa gcgatcatca agtatccgaa
     4381 gacaatcccg ggaaacatga gcgctacgcc ccaaaccagt atctgcaggg caggaatgga
     4441 gggagtgaac tcgcttccga aaaccaggac aattatcttg ggggcaagga cggtgaggct
     4501 tgttgccaga ggaagcgcca cgagaagcgt atagtcgaaa gacttctgaa atagcgtttt
     4561 caacttctct tggttgatct ttgagtattc cgacatgaac gggaagagtg aggttgccag
     4621 cgccagagga aacaccacgg acagatacac gaatttgtag gatgcgttgt aatagcccac
     4681 ctcttcagca cctctgatgt gctccagaat gagcatgtcc acagtcatat aaaggctgac
     4741 gactgaatag gcacacccca aggggagcga agatcgaagg atccttttcc aaaaatcgag
     4801 atcaagactc cagctgggtg catggaggaa ttcccgaaca agcgccagaa ccacgatcaa
     4861 gttcacgaaa ttggcaacaa caacaaccag gatgaaatgg atcagtcttc cgtcgaaaaa
     4921 aatgaccaga aggctgagaa gtagagccaa gatgtccttc accgcggtgg caaagacacc
     4981 tcgctgcatc gacagattca ctcggaagac gatgttccct gtcgaactga gcgaaagaaa
     5041 cagaagagag atggaggcaa gcgcgaaaca gcgcctcaag tcttccgcat acccaaggat
     5101 agcgacagca gcgatcgaga tggcgataac agcaagccca agcactgtct tcagaatgag
     5161 gagttcgatc accgcttgag cgatcctcga gcgatccttg gagagctcgc gcacaacgat
     5221 gtcgttggtt ccaaaatcag tgagagtggc aaagagcgcg ataatcacaa atatgaggga
     5281 gtatttcccg tattcctcgg cacccaagta tcgggggatg actgccagca gagcaagact
     5341 caggacaaga tggactgcct gaccaacaat ctgccaggaa gtgtttcgta ttatgcgtcc
     5401 gatggcgctc atcgtttctc catagcgatg cagtactgct aataagcgcc ccggcgcagc
     5461 agaactgtgg ggattgtctt cagaagaatc tttatgtcca gccagagcga ccaggtatcg
     5521 acatatttga catccatttc catccatcgt tcaaagtcga tcttgcttcg tccaccgact
     5581 tgccagaagc aggtgattcc tggcttcatg ctgaatcgct tgctctgcca ctctgagtca
     5641 aacccggcat agtcccgcac gggaagaggg cgaggcccga caagactcat gtcacctttg
     5701 aacacattcc aaagttgggg aagctcgtca atgctggtgc ttcgcagtat cctcccaatc
     5761 cctgtgaggc ggggatcgtt tttcatcttg aacgtcggcc catcggtttc gttcaggtgc
     5821 tccagagagc tgatgagcgc ttccgcatct tcaaccatgg ttctgaattt gaccaggttg
     5881 aagatcctct tgttgtagcc gactcgtttc tggatgaaga aggctggccc aggggaaagc
     5941 actttgagcg tcacatagac cgcaagcagc agtggcaggg tgagcaccaa caggaaggca
     6001 gagaccacaa tgtcaaacaa tcgcttcacg agtatctgtg gatcttcgta cgggctggag
     6061 cacagagtga tgacaggcat gtcgtccagg agcgagaagc gaaagttggc tgaggtcagt
     6121 gaaaagacac cgcgaaggaa tctgacgatg ataccgtgtt ctgcacaaag cctgacgatg
     6181 ccttcgatct gttcgtagta ggacttgatg ggcaggctga tgatgacttc gtcgacaacc
     6241 gagtttctca ggacttgtgg gatgtccttc agcgttccga tcaatcggct ctggaattgt
     6301 cccttcagaa ggctcggttc aggtgcatca tccacaaatc cgaccagcac atagcctagt
     6361 tcgggtcttc gttcgatctt ttctgcgaac tgttgagcgt tgcttcgact acccgcaatc
     6421 agcacgaaac gacgattcat gccattgcgc cgtagcgttt cgagcatgtt gcgcagcagc
     6481 aacctgccca agaccatgat gcagagcaac agcccccata accaag
//
LOCUS       DAOQUC010000028         6512 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_13038_length_6512_cov_3.275670, whole genome shotgun sequence.
ACCESSION   DAOQUC010000028 DAOQUC010000000
VERSION     DAOQUC010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6512
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13038_length_6512_cov_3.275670"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            244..1455
                     /gene="thrC"
                     /locus_tag="PKH07_00920"
     CDS             244..1455
                     /gene="thrC"
                     /locus_tag="PKH07_00920"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HNT33541.1"
                     /translation="MIQHSLLCTSCRREYPFGEIRLRCQQCGEPLELPKVSDGAINVA
                     DSLTQTMLERYSAFFPFASIDPLLSLGEGFTPLSESPRLSSDLGVRRLLFKNETLNPT
                     WSFKDRGTIAGIQHAAAIGCSRIGTVSTGNMAVSVAAYGARAGMETFVLVSDSIPEEK
                     ISPIAIHQPHLIRVNGDYGNLYFESVRIGTDLGIYFLNSDVPLRVEGSKTIAFEICEQ
                     LQLQVPDYVIVPTSAGGNLRGIIKGFEEFVLCGLIPRMPTIVCAQASGCAPIFNAWST
                     GKDSIERVQAPHTIAHAIENPFPPSGNQALRKIREHDGLCIAVSDDAILQAQRTMARE
                     GIFGQPAAAVPLAALRQLRKEGTVTEDATVVCVVTGCGLKYTAALREQPISTTHCTLE
                     QLPSVLASPRA"
     gene            complement(1704..4301)
                     /locus_tag="PKH07_00925"
     CDS             complement(1704..4301)
                     /locus_tag="PKH07_00925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNT33542.1"
                     /translation="MQSRTNSPEMRCQFLSRVSWPSICTFLLLLGIYFFAVGMRTASF
                     LAQGQDHRQQFWVESAQHFRNVQMVFETGDIPQVDIDLEYPDGLVTRSHTVHGEYLLG
                     YLARWLPYERVCAFLEDRLPRQGLARNFVQLFLPLQSPDLSGFVRYCVRYFNALIVFA
                     VFLVTVYFSRSRGLGLLTAFSYATSFPAISRALGDTFYHEHIALPCLAFHLVFLVRAL
                     NDSRWFDILMGWFFLFLALLFWKVIGFYALALNAYFALSFLADRMCSCKVRFLAVSTL
                     GNLLTSLLLNIHLNYDHYWFSPTMACAYAVLIASFVQHRIPKGRRLWSLALFFVVLVA
                     VIRITPEGERYTHVWDTLFAKLRYWTKPADPSLLSYEARHYWVEPYTSPPMFWFLQEV
                     APLLLLTLIPLCWALHRIRKGTLEHGKEIWVFLSLGFLFFYLLFFKMKTFVAILFPPL
                     VALSFSRQPLMHLWDKPFRRTWSWIAGGVFGGLALVYVTLLPVSLGLSRSADTWRRFA
                     LQWPYGKESFLLSLALIALGTVLATCVVRKESLRNPFRRPFIGVVLLSAVSFLYAYET
                     LSWNYSVVALGLHSGGIRQTNAKKFTRVASSRSVMELLAWIPANTAPRDSFLCEFVMS
                     PSIITYAKRPVNQHCFFECDMRDKFRLLVENFYEPEEVMYEFCRKYQTTFLVYNAHIL
                     LRNDPEMSFRYIANKMDFREDWLAYQMHFRPETLQHFQLEYQNDFIRVFRVLEPDQTP
                     SREATFRFSPLFAESLFERYCPRSDSGERDTAAYLYSLVEAYDLYDQAVGALKSPGGE
                     GYGQVLLQQSLAKSELIPDAHCLLAQMFLRAGQFELAKASVEKALAINPEDPLAQKLL
                     AAMMSGMRP"
     gene            complement(4276..5331)
                     /locus_tag="PKH07_00930"
     CDS             complement(4276..5331)
                     /locus_tag="PKH07_00930"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNT33543.1"
                     /translation="MRCAAVACFSVLIFSILICGCSKPPDELYDEAVVAMSRGDKEKA
                     ERKLRTAAHQDPEFVEAHYQLGQLLEGKGNVPEAIACYEKVTSLRPTHAAAKFRLGEI
                     YFRQDEFDKALSQLQPMTEGNAVYSLQDKTRATQYSKMIQANKDAVEKIAELEKQSTS
                     DPSILDELADAYHLYGKLLVDRQNPERALEYQKKGSDVRRELLQRLEEEFRQKPSDRK
                     LATRLAELYYKEAEVVLLTGDLTKGVDLLKKSIECDPRIAKNYYALAQLAEKEGSFSR
                     EQILGYVRKAVDLDPGNAVYRFSLAGFYCGDKRYQEGKRELEKIIESSPDPALKLQAK
                     ARLAQVEKEIAEQNKQP"
     gene            5623..>6512
                     /locus_tag="PKH07_00935"
     CDS             5623..>6512
                     /locus_tag="PKH07_00935"
                     /inference="COORDINATES: protein motif:HMM:NF019845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HNT33544.1"
                     /translation="MRQAKLIGASQIGFFHDAPVPEPKPGTARVRIKAVGICGSDKHL
                     FLHGKIGETQISEPFVIGHEAMGVVDAVGEGVVRNLVGRRVAIEPAIPCHHCECCIRG
                     DMNLCLNIRFLGHPPTPGAFQEFIIHPADLLEPVPDSVTDAGAVMLEPLAVVVHAFDL
                     GRIRFGSRVAVLGCGPIGLLAVWTARMLQPSVLLATDILDYRLDVAKSLGASHVGNPS
                     RQDIVAHAREITQGKGFDTVIEAATSDDTGEQMTAIAGAGAKILIIGITLSDRISFCH
                     ATARRKGLTFFMVRRSRNTL"
BASE COUNT         1631 a         1778 c         1675 g         1428 t
ORIGIN      
        1 acttccttca catccgtcct ggctccaccc tctggtggcc agttaaacag caaatccaca
       61 atggcaatct tactcatagg caaggaaacg tacctttttc gaacaaaacg tgtcaagcac
      121 gcataccgac aaaactgcta tccatcaatt ggctgcagtg ccacattgag tcgcggcctc
      181 tccaatgcta tatagagaag gagacccaga gaaatccgtc aagacagggt ttgggagaat
      241 aggatgatac aacacagtct cttgtgtacg tcttgcagac gggaatatcc cttcggggag
      301 attcgcttac ggtgccaaca gtgtggagag cctcttgagc tgcccaaggt ctccgatgga
      361 gcaatcaatg tcgccgattc tttgacacag accatgcttg agagatactc ggcgttcttc
      421 ccttttgcca gcattgatcc gcttctgtct cttggtgaag gcttcactcc cctgagcgaa
      481 tcgccccgac tgagttcaga cttgggcgtg agacggctgc tcttcaagaa tgaaacgctc
      541 aatcccacat ggtcgttcaa ggatcgagga acgattgcag ggattcagca tgccgcggca
      601 atcggctgct cacgcattgg cactgtttca acgggcaata tggctgtctc ggtcgccgcg
      661 tatggagcac gcgctgggat ggagaccttt gtcctggtca gtgattcaat tcctgaggaa
      721 aagatttcgc ccatcgccat ccatcaacca catttaatcc gtgtcaatgg agactatgga
      781 aatctctatt ttgaaagcgt tcgtattggt accgatcttg ggatttactt ccttaattca
      841 gacgtgccac tgcgggttga aggctccaag accattgcct tcgagatctg tgaacaactc
      901 caattgcaag tccccgatta tgtcatcgtt cctacaagtg ctggtggaaa tcttcgcgga
      961 atcatcaaag gatttgagga gttcgttctc tgtggtctca tcccccgaat gccaacaatc
     1021 gtctgtgccc aagcgtccgg atgtgctcca atcttcaacg cctggtcaac tggaaaggat
     1081 tccattgaac gtgtgcaggc tccccacacc atcgcccacg ccatagagaa tccttttcca
     1141 cctagcggaa accaggcgct tcgtaagatt cgagaacatg atggactctg tatcgccgtc
     1201 agcgatgatg ccatcctcca ggcacagaga accatggcgc gagaagggat tttcggacag
     1261 cccgccgcgg cggttcctct ggccgctctc agacaactga ggaaagaggg aaccgtgaca
     1321 gaggatgcca ccgtggtctg cgtcgtcaca gggtgcggtt tgaaatacac ggcagcactt
     1381 cgagagcaac ccatatcaac aacgcactgc acactcgagc agctcccgag cgttcttgct
     1441 tctccgagag catagggtgt ggcagccctg ccctccggaa ggtcgcagca agcagtgccc
     1501 ctacaggaac aacacagatt gtggcgatgc attcatgccg cttcctcgtt cgtaattacc
     1561 ccgtcagagg tgataaggat ttgcccttac ggacacacta cgaacctttc gcgcaagcaa
     1621 gcttgcgcac tccaaaatct tcttcccagg agtgcgggag accgttgccc ccacccagga
     1681 tcccggtctc gggggctaca ccctcaggga cgcatgccgg acatcattgc agccaacagt
     1741 ttttgtgcga gggggtcttc cgggttgatc gcaagtgcct tttcaaccga ggcctttgcc
     1801 agttcgaact gtcccgcacg aagaaacatc tgcgccaaca ggcaatgcgc gtccggaatc
     1861 agttcactct tggcaagcga ctgttgcagg agcacctgcc cgtagccttc cccaccggga
     1921 cttttcaacg cccctacggc ttggtcgtag agatcatagg cttcaaccag agagtagaga
     1981 taggcagccg tatcccgttc tcccgaatcc gagcgagggc agtagcgttc aaacaacgat
     2041 tcagcaaaga gcggactgaa tcgaaatgtt gcctctcggc ttggtgtctg gtcaggttcc
     2101 aacactcgaa acactcggat gaaatcgttt tggtactcca gttgaaaatg ctgaagtgtt
     2161 tcaggtcgga agtgcatctg atatgccagc cagtcttcac gaaaatccat cttgttggcg
     2221 atgtatcgaa aagacatttc gggatcattg cgcagcaaga tgtgcgcatt gtagaccaga
     2281 aaagtcgtct ggtactttcg gcagaattcg tacatcacct cttccggctc atagaagttc
     2341 tccacaagca gccgaaactt gtcccgcatg tcgcattcaa aaaagcaatg ctgattcacc
     2401 ggacgcttgg cataggtgat gatggacggc gacatcacaa attcgcaaag aaaagaatca
     2461 cggggcgccg tgttggctgg gatccaggcc agcagttcca ttacagaacg ggaactagcg
     2521 acgcgggtga acttcttggc gttcgtttgt cggatgccgc cgctgtgcag acctaaagcc
     2581 actaccgagt aattccacga caacgtttcg taggcgtaga gaaagctgac agcactgagg
     2641 agaacgacgc cgataaaggg acgtcgaaac ggatttctca gggattcttt ccgaacaacg
     2701 caggtcgcca ggactgtccc caaagcgatc aaagccagcg acagcaggaa actttccttc
     2761 ccgtatggcc attgaagcgc gaatcgtcgc caagtgtccg ccgagcgact caatcccaat
     2821 gagacaggaa gcagcgtgac gtagaccagt gccagcccgc caaacacacc gcccgcaatc
     2881 caagaccagg tacgccgaaa cggtttgtcc cacaggtgca tcaaaggctg ccgtgaaaag
     2941 gacagggcaa ccaacggcgg aaagagaatg gccacaaatg tcttcatctt gaagaacaga
     3001 agatagaaga agaggaaacc aagagaaaga aagacccaaa tctcttttcc gtgttcgagc
     3061 gttccttttc ttattcgatg cagcgcccag caaaggggaa tcagtgtcaa cagaagaaga
     3121 ggcgccactt cctgaagaaa ccagaacatc ggaggactgg tataaggctc tacccagtaa
     3181 tgtctcgctt cgtaggaaag cagcgacgga tccgcgggct ttgtccaata gcgaagtttg
     3241 gcaaaaagag tatcccagac atgcgtgtac cgttctccct caggggtaat tcgaatcacc
     3301 gcgacaagca ccacaaagaa caacgcgaga ctccaaaggc gtcttccctt tggaattcga
     3361 tgctggacaa aactcgcgat cagaacggca tacgcacaag ccatggttgg agaaaaccag
     3421 tagtgatcat agttcagatg gatgttcagc agcagcgaag tcagaaggtt tcccaaggtg
     3481 gaaacggcaa ggaatcgaac cttgcacgag cacatcctgt ccgcaagaaa actcagggcg
     3541 aaataggcat tcaacgcgag tgcatagaaa ccgatcacct tccagaacag aagcgcgaga
     3601 aacaagaaga accagcccat aagaatgtcg aaccatcggg agtcgttcag agcacgaacg
     3661 agaaacacca gatgaaaagc aagacacgga agcgcaatgt gctcatggta gaaggtgtct
     3721 cccaacgctc ttgaaatggc gggaaaactc gtcgcatagg aaaacgccgt cagcagtcca
     3781 agcccccgac ttcgactgaa gtagacggtg acaaggaaga cggcaaagac gatcagggca
     3841 ttgaagtatc ggacacaata ccggacaaac cctgacaagt ccggactctg aagcgggaga
     3901 aacagttgga cgaagttcct ggccaaaccc tgcctcggaa ggcgatcctc cagaaacgcg
     3961 cagactcttt cgtaaggaag ccagcgagcc agatagccca atagatattc cccatgaacc
     4021 gtgtgtgaac gtgtcacaag cccatcagga tattccaggt caatgtccac ttgggggatg
     4081 tcccctgttt cgaagaccat ctgaacattg cggaaatgtt gcgcggattc cacccagaac
     4141 tgttgtcggt gatcctgtcc ctgagcgagg aatgacgccg ttctcatccc caccgcaaag
     4201 aagtatatcc ccaacagcag caggaaggtg caaattgaag gccaggaaac gcgggaaaga
     4261 aactggcagc gcatctcagg gctgtttgtt ctgctctgca atttccttct ccacctgggc
     4321 aagacgagcc tttgcctgaa gtttcagggc agggtcggga ctggactcaa taatcttttc
     4381 cagttcccgc ttgccctcct gatagcgttt gtccccacag tagaatcctg ccagggaaaa
     4441 tcggtagacc gcatttcccg gatcgagatc gacggccttt cgaacgtagc cgagaatttg
     4501 ttcccgagag aaggagcctt ccttttcggc caactgtgcc aaggcgtaat agttcttggc
     4561 aattcttgga tcgcattcga tggatttctt cagcaggtca acacccttgg tcaggtctcc
     4621 cgtcaggagc accacttctg cttccttgta gtacagttca gcgagccgcg tcgcgagttt
     4681 acgatcagag ggtttctgac gaaactcctc ttccagcctt tgaagaagtt cccgacggac
     4741 atctgaaccc ttcttttgat actccaatgc gcgttccgga ttctgtctat ctaccagcaa
     4801 ctttccatag agatgatagg catcagccaa ctcatcgaga atgcttggat cgctcgtgga
     4861 ctgtttctcc agctcggcaa tcttctcgac ggcatccttg ttggcctgga tcattttgct
     4921 gtattgcgtt gcgcgggtct tgtcctgaag actgtacacg gcattgcctt ctgtcatggg
     4981 ctgaagctga ctgagggctt tgtcaaattc atcctgacgg aagtagatct cacccaagcg
     5041 gaattttgcc gcagcatgag tgggacgcag cgaggtcacc ttctcatagc aggcaattgc
     5101 ctctggcaca ttgcccttac cctccagtaa ttgtcccaac tggtaatgcg cctcgacgaa
     5161 ctctggatcc tgatgcgcgg cggtgcgcag tttccgctcc gccttttcct tgtctccgcg
     5221 actcattgcc acgaccgcct cgtcgtaaag ttcatccggc ggcttggaac acccgcagat
     5281 cagaatcgaa aatatgagca cactgaaaca ggcaacggct gcgcaacgca tgaagccctc
     5341 cattctgctc cgcaaacaag caatctcgtt tcacttccga caggagtttc accaaaacgc
     5401 tcctgtttcc aatcacttct gaactcggga gagcgacaca cgtcgaaatt gcccgacgga
     5461 caaccgaaca aagcctcccg aatcaagaga agataacagc accatcacaa agacgcaagc
     5521 catgcgaaac ggtgcccaat atattctatc cctcactgga cgtttcggtc ttctctttgt
     5581 atgattcctc tcatcttgat tctttacatg aggcaggaga agatgagaca agcaaagctg
     5641 attggcgcaa gccagattgg tttcttccat gatgcgccgg ttcctgagcc gaaaccaggc
     5701 accgcccggg ttcggatcaa ggcggtgggt atctgcggtt ctgacaagca cctttttctc
     5761 catggcaaaa tcggcgagac ccagatttcg gaaccctttg tcattggtca cgaggcgatg
     5821 ggcgtcgtgg atgccgttgg cgagggcgtc gttcggaacc ttgtcggaag gcgcgtcgcc
     5881 attgagccgg caatcccctg ccaccattgc gagtgctgca ttcgagggga tatgaatctg
     5941 tgtctgaaca ttcgttttct gggtcatcca cccacaccgg gagcctttca ggaattcatc
     6001 atacatccgg cggatcttct ggaacctgtc ccggattctg tcacggatgc cggagccgtg
     6061 atgcttgaac ctttggcggt tgtggttcac gccttcgatc tagggaggat tcgatttggt
     6121 tcgcgtgtcg ctgtcctggg ctgcggtccc attgggcttc tggcggtctg gacggcaagg
     6181 atgcttcagc caagtgttct gctggccaca gacattctgg actatcgcct cgacgtagca
     6241 aaaagtctcg gagcgtcaca cgtcggaaat ccttcccgac aggatattgt cgcgcatgcc
     6301 agggagatca cacaggggaa gggttttgac acggtcatcg aagcggcaac gtccgatgac
     6361 acgggtgagc agatgacagc catcgcaggg gcaggagcca agattcttat catcggaatc
     6421 accctttctg atcggatttc cttctgccat gccacagcga gaagaaaagg gttgaccttc
     6481 ttcatggtga gacgatcacg caacactctc aa
//
LOCUS       DAOQUC010000029         6480 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_13134_length_6480_cov_2.887471, whole genome shotgun sequence.
ACCESSION   DAOQUC010000029 DAOQUC010000000
VERSION     DAOQUC010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6480
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13134_length_6480_cov_2.887471"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(62..2023)
                     /locus_tag="PKH07_00940"
     CDS             complement(62..2023)
                     /locus_tag="PKH07_00940"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNT33545.1"
                     /translation="MRLRCNGIAGFDGISGKQGRVISGMSDEAGQFHLSPAQLRRSWR
                     IVTWAGLLGTVYYILCINGAPRIKFLTEMKATAFDFGLIAGLSSLALAFQVIGSLAAN
                     RLKHRKPVWMIIAITHRLVFINVLMAPMLFGNDRLRLWWIILLLFGHDLLAQIGVPLW
                     MSWMADLVPKETMTRHWASRQRLITSINILVMIAVALFFDRYEKSGQIVRGYTLMASV
                     GVIIGIVDILLFLRVPEPPNERALRTPLLEAVSQPLKDFHFRAFLRYYVCWYFAVFVG
                     SPFMALYMIDHLGMDARTVQLISAFGLLGVVLVSRLWGLLCDTYGYRPVIRILTVGKS
                     FTPLFFFFAPAILSISIPMMAVLMFVDGMMNSGTTLAFQGVLLKSTPKKNRAMYIAAS
                     NFMALGVAASIAPILAGKIIDILNELPSLNIGFYRLNGYHAVFALSAALRFSVIPLAN
                     RLHQPKSFPIKRVIGQINFENAFRVTRLIYHLHESSKAHKRVASAQALGRLKNPLAIS
                     DLINALNDPSQNVREAAADALGAIGASEAAEPLSRALFDPRSGIAPRAARALGRIAGV
                     QSLKALLTNLRNADSVALHETIDSLADIGHDAAILPLICFFREVQDPDLRIHVARALG
                     KISKSESPEEVLSMLMGRRSLPQPGLNRT"
     gene            2170..2910
                     /locus_tag="PKH07_00945"
     CDS             2170..2910
                     /locus_tag="PKH07_00945"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: protein motif:HMM:NF012554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA];
                     GO_process: GO:0006183 - GTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006228 - UTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HNT33546.1"
                     /translation="MHRTNMDQTLVLIKPDALKNSLTGYVLSQLSEFHTGLRFAGAKI
                     VNVSKMLAEEHYAEHLGKVFFPSLIEYITGASHYPDQPWKRRVIALVYQGENAPKRIR
                     ELAGPTNPHAAREEKPGSIRSLGTLVDIKDASGKVIGQRIDNLIHASATLEEAEREIK
                     LWFKPCDIPPLMRPYQTEICHEHYYYKDGTILHQNYEPGSVCLMAPGDTVWASDMEAL
                     KLHTQGKPTATTIGSVAAKYLINQDSAE"
     gene            2954..5200
                     /locus_tag="PKH07_00950"
     CDS             2954..5200
                     /locus_tag="PKH07_00950"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNT33547.1"
                     /translation="MEIDFLRHYGLDKTVTDLLRDHYGADLLPVQETAFVENNLFDGT
                     NMVVSAPTSSGKTLIGEVAALHHIEHDRRALVLVPTKALAYEKYEHFQRLYQQPLGIR
                     VVLSSRDHRERDEDIGTGKYQIAVVVFEKLLGLLARYPALLESVGVAVVDELQTIFDP
                     ERGARIEQLVTHLKRAKSVQLVGLSAVLDGARLAEWLSAKLVEETQRPVELRQGVLCR
                     GRFYYREYNSGEEGVEELFSSDSDNEAESLLEAAMHLAERGEQTLIFVPQRHLCILWA
                     DLLAQQVSLPAASEALQQLQTLETNSVGSRLQDFLHKGIAIHNSDLTWMQRELVEKAL
                     QCGEVRIAVATSTLSEGVNLPVVNTLLCRQSYQTTVPSMLRGQPPQLGRLTREQFQNM
                     TGRSGRMGRCLFGRGMIAVGFEGEVRGVLARYLRDPWARPQPILSQVSLESTALGLLA
                     TRVASSKEELVGFIANTLSGHETGADWAESGMNSSVSTLMRSGLCIEAQNRLTCSATG
                     MLIAQSGLEPVTGLWFLDWLDNEPETGYEETDLLLLAALCPLDNRAFFPFTEREWKGH
                     FFVRRLWERLDQEGERSHGLLESLLGGEETLPTFDTARSIKRAAFLIDWCSDQATEDI
                     EHRYGVMEGMAQRIGEECSWLLQGLADLARLKSATDQTIRAAAELVLRSKLGLSKEWK
                     DFGKLNATGLSRDMVRRLVSEGLTHPSELRTIPAEQMAQWIPASVVSRIQDYLRSNSL
                     AAKGQDNQ"
     gene            5375..>6480
                     /locus_tag="PKH07_00955"
     CDS             5375..>6480
                     /locus_tag="PKH07_00955"
                     /inference="COORDINATES: protein motif:HMM:NF017078.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE4 domain-containing protein"
                     /protein_id="HNT33548.1"
                     /translation="MPIRWKLILLLGMLIGLFVGSQQLLHSLIFSPSFKNLEQQESRR
                     NIKRCVQCIEREIWHLDEYFGSDWAAWDDTYEFVEDRNEDYIESNLVDTSFTDNDINL
                     IYILNATHEVVWGKVMDLATEREITLPEFHKGTWRHDHPLLAPRSPGEYVAGVMIGQA
                     TPLLISSRPILSSHMQGPSRGTFIMARFLDANLIQTLSKQVDFDFEVQVLGAMRLDAE
                     SQAALYQILAGEEFCVSIIDDVRLNAYALLRDIQDQPALLIRAELPRDISMTGLRAMR
                     SAHGSIAVLGLLQLLAALLLIERTVLARLTNLERFVRQVGITADASSRLSKMGGDELG
                     QLAHNINAMLESLEKAQAELRDTEAQLRQSQEVL"
BASE COUNT         1612 a         1693 c         1791 g         1384 t
ORIGIN      
        1 tgttgtctgc cagcctttca ggtcccactt catatctgcg gaatatgcag cgtaacaacc
       61 atcacgttcg gttcaagccg ggttgaggca atgaacgacg acccatcagc atgctgagaa
      121 cttcttcggg cgattcagac ttgctgattt tccccaacgc tcttgcgaca tgaattcgga
      181 gatccggatc ctgaacctct cggaaaaaac agatcagagg cagaattgcg gcgtcatgtc
      241 cgatgtctgc caaagagtca atggtctcat gcaacgccac agaatccgcg ttgcgaagat
      301 tcgtcagcag ggctttcagc gactgaaccc ccgcaatacg acccagtgcc cgagctgcac
      361 ggggggcgat gcccgaacgg gggtcaaaca gtgcgcgcga gagaggttcg gctgcttccg
      421 aggcaccgat cgcgcccagt gcgtcggccg ccgcctctcg gacattttgg ctgggatcat
      481 tcagggcatt gatgaggtct gagatcgcca acggattctt gagtctgcca agcgcctgag
      541 ccgaggcaac acgtttgtgc gccttggaag actcatggag atgataaatc aagcgagtca
      601 cgcggaaggc attctcaaag ttgatctgac cgatcaccct ctttatcggg aaggatttcg
      661 gttggtgaag tctgttggca aggggaataa cagaaaaacg caaggcggca ctcagtgcaa
      721 aaacggcatg atagccattg aggcgataga acccgatatt cagggatgga agctcattga
      781 gaatatcaat gatcttgcct gccagaattg gggcgatgga agcggccacg ccgagcgcca
      841 tgaaattcga ggcagcgata tacatcgccc tgtttttctt gggtgtggat ttcagcaaca
      901 ctccctgaaa agccagcgtt gtcccagagt tcatcatgcc gtcaacaaac atcagcaccg
      961 ccatcatcgg aatgctgata ctgaggatcg cgggggcgaa aaagaaaaag agaggcgtga
     1021 aagattttcc caccgtaaga attcgaatga ccggacgata gccatacgtg tcacaaagaa
     1081 gcccccacaa acgcgagaca agaaccactc ccaacaaacc aaaggcactg atcagctgga
     1141 cggttctcgc atccatcccc aagtgatcga tcatgtaaag agccatgaaa ggtgaaccga
     1201 caaagacagc gaagtaccaa cacacatagt acctgagaaa agcccgaaaa tggaagtcct
     1261 tgagcggctg cgacaccgcc tcaagcaagg gggttctcag cgcccgctcg ttgggcggct
     1321 ctgggacgcg cagaaacagc agaatgtcca cgatgccaat gatgacgccc acggaggcca
     1381 tcagagtgta gccacggacg atctgacccg atttctcata gcgatcgaaa aacaaagcca
     1441 cggcaatcat gaccaggatg ttgatgctgg tgatcagcct ttggcgggaa gcccagtgcc
     1501 gtgtcatggt ttccttggga accagatccg ccatccacga catccaaagc ggcactccaa
     1561 tctgcgcaag tagatcatgc ccaaacagaa gcaggatgat ccaccacaga cgcagccggt
     1621 cattgccaaa cagcatgggc gccatcaaga cattgataaa cacgagacga tgcgtgatgg
     1681 caataatcat ccagacgggc ttacgatgct tgagacggtt ggccgcaaga ctgccgatca
     1741 cttgaaaggc aagcgcgagg gaactcagtc cagcgataag accgaaatca aacgccgtgg
     1801 ctttcatctc ggtcagaaat ttgattcgag gagcaccgtt gatacaaagg atgtagtaga
     1861 cggttcccag aagccccgcc caagtgacaa tgcgccaact ccgcctcagt tgggcgggag
     1921 agagatgaaa ttggcctgct tcatcgctca tacctgaaat gacacgaccc tgctttccgg
     1981 aaatgccatc gaacccggca attccattgc aacgcaacct caagggaaga tatccctttt
     2041 gttgctggga tttcaagtga gatgtcgcac ggtcattcgc gaccgttgac aggttctttt
     2101 cgaaaggata ccttacccgc tgtcaatcag gagaatgttt gaccgtcatt tgtgaaagga
     2161 accgtttcta tgcaccgaac aaacatggat cagaccctgg tgctgatcaa gccagacgcg
     2221 ctgaagaatt ccctgacagg ttacgttctg tcacaattgt ccgagttcca cacgggactg
     2281 cgtttcgccg gagcgaaaat cgtcaacgta agcaaaatgc ttgctgaaga gcactatgcc
     2341 gagcatctgg gcaaggtatt cttcccctcc ctcattgagt acatcacagg cgcttcccac
     2401 taccctgatc agccgtggaa gcgccgagtc atcgccttgg tctaccaagg cgagaatgct
     2461 cccaagcgga ttcgagaact ggcgggccca accaacccgc acgcggcgcg cgaggaaaag
     2521 ccaggcagca ttcgttcgtt gggaacgctc gtagacatca aggatgcctc aggcaaagtc
     2581 atcggacaga gaattgacaa tctcattcat gcttccgcca cactcgaaga agccgagaga
     2641 gagatcaaac tctggttcaa gccctgcgac attccgccgc tcatgcgccc ctatcagacc
     2701 gagatctgcc acgaacacta ttactataag gacggcacaa tcctgcacca aaactacgaa
     2761 cctggaagtg tctgtctgat ggctcccggg gacacggtgt gggcgtccga catggaagca
     2821 ctgaagttgc acactcaggg gaaaccgacg gcaaccacta ttggcagtgt cgcggccaag
     2881 tacctgataa accaggattc tgcagagtga tctgccgtct cgtgtcgcgg tctcttggtt
     2941 gaaggaaccc aacgtggaaa ttgatttcct tcgtcattac ggtttggata agaccgtaac
     3001 cgatcttctt cgagaccatt acggcgcgga tttgctcccc gtccaggaga cggcattcgt
     3061 tgagaacaat ctctttgacg gaaccaatat ggtcgtctct gctccgacct cttcagggaa
     3121 gacgctgatc ggagaggtcg ctgctctgca ccacatcgaa cacgaccgac gtgccctggt
     3181 attggttccg accaaggctc ttgcctacga aaagtacgaa cacttccaga gactttacca
     3241 gcagccgttg ggtatccggg tcgttctttc gtctcgagac catcgcgaaa gagacgaaga
     3301 cattggtaca gggaaatacc agattgcggt cgtagtcttc gagaagctgc tgggacttct
     3361 ggcgagatat ccggcgcttt tggaatcagt cggagtggcg gttgtggatg agctgcaaac
     3421 gatcttcgat ccagagcgag gagcacgcat cgagcaactg gtaacccatc tgaaaagagc
     3481 gaaatctgtc cagttggtag ggctgtccgc cgtgctcgat ggcgcacgtc tggcagaatg
     3541 gttgagcgcc aagctggtcg aagaaaccca acgaccggtc gaactgcgcc aaggggtgct
     3601 gtgccgggga cgtttctact atcgggagta taactccggc gaagaaggtg tcgaggaact
     3661 gttctcgagc gactccgaca acgaagccga gagccttctt gaggctgcca tgcacctcgc
     3721 ggaacgcgga gagcagactt tgatcttcgt tccccagcgt catctttgta ttctgtgggc
     3781 tgatcttttg gcgcaacaag tctcgttgcc tgccgcatcc gaagccctgc aacaactaca
     3841 gacattggaa accaattctg ttggatcacg gttgcaggat ttcctccaca aaggcatcgc
     3901 catccacaac agcgatctga cctggatgca gagagaactg gtcgagaagg cgctgcagtg
     3961 tggcgaagtt cggattgctg tggctacttc gactctctcg gaaggcgtca accttcctgt
     4021 cgtcaacacc ttgctgtgcc ggcagtcgta ccaaaccact gtgccttcca tgcttcgggg
     4081 acagccacca cagttgggaa gactgactcg agagcagttc cagaacatga ccggcaggag
     4141 cggtcggatg ggcagatgcc tctttgggcg gggaatgatc gcggttggat tcgagggcga
     4201 agttcgaggt gttttggctc gatatctgcg tgatccgtgg gcaaggccgc aaccgattct
     4261 gtctcaggtt tcgctcgaat caacagccct cggactcttg gcaacacggg tggcatcttc
     4321 aaaagaggag ttggtaggtt tcatcgcgaa cacactctcc ggccatgaga ctggagccga
     4381 ctgggcagaa agtgggatga acagttcagt cagtactctc atgcgttccg gactctgtat
     4441 tgaggctcag aaccgcttga cctgttccgc aacgggaatg ctgatcgccc agtcaggact
     4501 ggagccggtc acaggtctct ggtttctgga ctggttggac aatgaacctg aaaccggcta
     4561 tgaggagact gatctgctgc tgctcgccgc tctttgtcca ttagacaatc gcgccttttt
     4621 tccattcacc gaacgagagt ggaagggtca tttctttgtc cgaagacttt gggaacgtct
     4681 ggatcaagaa ggagagagaa gccatgggct gttggaatcc cttctgggtg gagaggagac
     4741 acttcccaca ttcgatacgg cgcgaagcat caaacgtgcc gcatttctga ttgactggtg
     4801 ctcggatcag gccaccgaag acatcgaaca ccgatacggc gtgatggaag gaatggcaca
     4861 gcgaattgga gaggaatgct cgtggctgtt gcaggggctg gcagacctgg cacgactcaa
     4921 gagtgcaacg gatcagacga tacgcgccgc agctgagctg gtactgagaa gcaaactcgg
     4981 gctttcaaag gaatggaaag acttcgggaa actgaatgcc accggactca gtcgggacat
     5041 ggttcgacga cttgtcagtg agggactgac ccatcccagc gagctccgga caattccagc
     5101 cgaacagatg gcgcagtgga ttccagcctc tgtcgtcagt cgaattcagg actatcttcg
     5161 aagcaactca ttggcagcga agggacagga caatcagtag ttgtctccga aagaacacaa
     5221 acaacctgtc gtttcgagct gtgttcgtct ctctcaacaa ggccgcctga cacatgaggg
     5281 acagcgaggt ggattggatg ctgtgttgaa ccagagtgaa gtccgccgta tactccatgc
     5341 tgtccgactc agtctgagag gtgtcaatca gaagatgcca atacggtgga aactcatact
     5401 cctgctggga atgttgatcg gtctctttgt cggttcgcag cagcttcttc actccctaat
     5461 cttctctcca agtttcaaga atttggagca gcaggagagc cgtcgaaaca tcaaacgatg
     5521 cgtccagtgt attgagcgag agatatggca tctcgacgag tactttggtt ccgactgggc
     5581 ggcctgggat gatacctatg agtttgtcga agaccgcaac gaggactaca ttgagtccaa
     5641 tctggtggac acttcgttta cagataacga catcaacctg atctacattc tcaacgccac
     5701 tcacgaagtg gtgtggggta aggtgatgga tctcgccact gagcgcgaga ttacacttcc
     5761 cgagtttcac aagggcactt ggcgtcacga tcatcccttg ctggctcccc gcagtcccgg
     5821 ggagtatgtg gcaggggtca tgatcggaca ggcaacgcct ttgctcatct cctcaaggcc
     5881 tattttatca agtcatatgc aagggccttc ccgaggaacc tttattatgg cgcgatttct
     5941 tgacgcgaac ttgatacaga ccctgtccaa acaggttgat tttgacttcg aggtgcaggt
     6001 gttgggagca atgagacttg atgcagagag tcaagcagcc ctgtaccaga ttctggcagg
     6061 agaagagttc tgcgttagca ttattgatga cgttcggctt aacgcctatg cactgctgcg
     6121 cgacatccag gatcagcctg cgcttttgat acgtgctgaa cttcccagag atatctcgat
     6181 gaccggtttg agggccatgc gctctgccca cggctcgatc gcagttctgg gtctgctcca
     6241 gttgttggca gctttgctgc tcattgaaag aacagttctg gcgcggctga cgaatctgga
     6301 acggtttgtt cgccaagtgg gaatcacggc agacgcttca tcaagacttt ccaagatggg
     6361 aggagacgag ctgggacagc tggctcacaa tatcaatgct atgttggaaa gcttggaaaa
     6421 ggctcaagcg gagttgcgag acactgaggc gcagctgaga cagtctcagg aagtgctcga
//
LOCUS       DAOQUC010000030         6478 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_13138_length_6478_cov_3.196014, whole genome shotgun sequence.
ACCESSION   DAOQUC010000030 DAOQUC010000000
VERSION     DAOQUC010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6478
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13138_length_6478_cov_3.196014"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1546
                     /locus_tag="PKH07_00960"
     CDS             <1..1546
                     /locus_tag="PKH07_00960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HNT33549.1"
                     /translation="GGTVKAIYFGGLPLACQLVERDIPEVTAVFAGDETPAECLRLLG
                     IDTRLLPSEIAAGATYDEDRLAFGRNLIQRADYRGIKRVDRKASKGFGTDQETLWDRI
                     HHGTQCGLPPLMRAHVGPYLPDRQSAVKLFFEWTKQLADSGLLDVLSIGTSQLTQSNF
                     GDDWGNRPNGGGVPLNSPEEFAETWRIARPMLVRSYAGTRDVVDLARMFERTIHIAWH
                     ALSLWWFSRIDGRGPYSVRDNLQQHLETLRFIASTGKPYEPNVPHHFAFRGADDVTYI
                     VSAVLAARAAKAQGVRTLVLQIMLNTPKATWGIQDLAKARALLQLVREIEDEHFHVFL
                     QPRGGLDYFSPDAEKAKAQLAAVTALMDDIEPHDPTSPPLIHVVSYSEGSHLADPAVV
                     NESIQVTRYALEQYRRLRARGHIDDMAKHPQVTERTAELLSEARTVLESIERTVPAPY
                     SAEGLYNILVQGFLPIPYLWECKDEFPKATAWSTRLMSGSVKVVDAQGRVITARERMR
                     LTTDPL"
     gene            1612..2760
                     /locus_tag="PKH07_00965"
     CDS             1612..2760
                     /locus_tag="PKH07_00965"
                     /inference="COORDINATES: protein motif:HMM:NF025330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HNT33550.1"
                     /translation="MKRLLFVFLLVPILCGCYREIKGEKPGETPSTVSPVAPPLLPVS
                     PMPPPVIPVLWKPLPKMIEVRYSFATEIVNGRIYAMGGLCFGNGLRMLSSCEVLDPES
                     KTWTQLGNMQVARSDFDSVLIGSRIYVMGGVYRDEFRGDVFLSSCEVFDTNTGTWECL
                     PDMNEARAAFRAVALNGKIYVMGGCNNLSEDLSSCEVFDPATGSWTMLASMNQARILF
                     ESVVLDGNIYVLGGRTDRGSYSDALSTCEVYDTNSNTWQALPEMRRPHDTFQAVVLNN
                     RIYALGGHSPYCEFFDPEDRSWTLISSMTQARRFFQAVVLKNKIYAFGGDSTNGFVST
                     AEVFDPTANQWFRLTEMSAPQGQFRVEVLRNSIYVIGGRACEYCEPPK"
     gene            complement(2864..3943)
                     /locus_tag="PKH07_00970"
     CDS             complement(2864..3943)
                     /locus_tag="PKH07_00970"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNT33551.1"
                     /translation="MEKRRLGQTDMVVTRIGLGTIPIIKESKWRAVRIIHKALDLGIN
                     FIDTARAYEDSEEKIGEVMKTRRSECFLASKSHFRTASETTEALETSLRNLQTDYLDL
                     YMLHDISAQDRWDLVFAPNGAFEAVKKAQQQGKIRYIGVSGHNVPMLMDLARMGEFSV
                     ILTCYNFMTLEPEYELIPLAQSKGVGVLVMKPNVGGVAFRLIRPGKDGQEPQPSDIKP
                     RDTLRFVLSNSGVDCALVGPKRVGEMVENYKFANRFEPMDETERQKFIESGQSLFPKM
                     PLCQDCGYCEPCPAGIPIAKLMVLYQNLCRLFFEWPRSRKEYHKLQPDVAACTDCGAC
                     EERCPQKLNIRERLRNAKKRLDHPV"
     gene            complement(3982..5988)
                     /locus_tag="PKH07_00975"
     CDS             complement(3982..5988)
                     /locus_tag="PKH07_00975"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNT33552.1"
                     /translation="MIWLVILLAVVLPEDRGGLADTLFLLITGGWFVFRLCVLVWHKI
                     RDQKVDIPHVTWAAFSLVILFFASLATYGSICQHTSFTALRLVVANALVFVAITEVGK
                     RAGVGMMLLLTLIGLLGTLIPLSPLNRWVHRFVFSLPHGSNLLDALLGRSGLLTLVFL
                     PPTVYVLLLVLSDLSCSAPVSQTDATGCETTLHRIIRKMFSSVGIVFTLCLWFLFAFT
                     VAWGNPKNLLFVTLPAALVVFGTARIGASSDRSRRAILLALTIFLSVALAHYSTAGFV
                     GRLVLETLKRSPGLSLGIQMAEPSIVRASVDNLPAGTGLGTLEDVLPQYRQLGQWRLV
                     WGGAYRVIQAEMGWTWLAVAVLLFSAALASFVERCLKMRKAGPDWRLGYLGSLLVSLL
                     MAGFADVRVHLLLCPLVWIILGALVAWRPAREKEQTSSQATAGSSSRSWVGRHLLVVT
                     RSFLLSLLLMAFVVNLHRSIRNKVGDLHFNRAAALVADPESRRAAILTELVEASEWSP
                     LDSRIDYEIARTFLRAFRNPPSSPGVQERQNARLALDTAIAKCPLRADYRGLLGQVLF
                     LEGDTAGALQMYQSAIQVDPLNALWRQSLADLYETMGNLPEAERHLRVLCDLLPFSGE
                     ARYNLARVLERQGWKQEALSIYRAAMEVQPGYLPAFDKDRESQY"
     gene            complement(5985..>6478)
                     /locus_tag="PKH07_00980"
     CDS             complement(5985..>6478)
                     /locus_tag="PKH07_00980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33553.1"
                     /translation="PLWYQKYVCGMGQDVAVVGANFLSSPWYEPMLLREGIQTTLPSE
                     IYQSRQEWMQAIWRKIISPTKASHRIYSTRPLEYNQLDCFMSPAAIPGFTWSPLFLLH
                     LRSAPFPLRQVPVKVDYSNVSERYLQYLPLPFLFQMDQVLMELDGYRRVDFGVWTPME
                     AQR"
BASE COUNT         1543 a         1758 c         1776 g         1401 t
ORIGIN      
        1 gggaggaacg gtcaaagcga tctatttcgg tggtctcccc ttggcatgtc aactggtcga
       61 gcgcgacatt cccgaagtga cagcagtctt cgccggtgat gagactcccg ctgaatgcct
      121 tcgactgctg gggatagata caaggcttct gccaagtgag attgctgccg gagcaacgta
      181 tgacgaagac cgcctcgcgt ttggcaggaa cctgattcaa cgcgcagact atcgcggcat
      241 caagcgcgtg gacagaaaag cgtcaaaagg gtttggcacc gatcaggaga cgctttggga
      301 tcgcatccat cacggaactc aatgcggact gccccctctc atgagggcgc acgttggacc
      361 gtatcttccc gaccgacaga gtgcggtgaa actgttcttc gaatggacaa agcaactggc
      421 ggattccgga ctgctcgacg tgctttccat cggcacttcg caactcacgc aatccaattt
      481 cggcgatgac tggggaaacc gacctaacgg tggtggagtc cctttgaact ctccggaaga
      541 gttcgctgaa acgtggcgta ttgcccgtcc gatgctggtt cgttcctatg caggcacacg
      601 cgatgtggtt gaccttgccc ggatgtttga gaggacgata cacattgcct ggcatgcctt
      661 gtccttgtgg tggttttcca gaatagatgg aagagggccg tactcggttc gagacaatct
      721 ccagcagcat ctggaaactc ttcggttcat cgcgtccact ggcaaaccct atgaacccaa
      781 cgttccacac cacttcgcct ttcggggtgc cgatgatgtc acctacatcg tctctgcggt
      841 gcttgccgca agagccgcaa aggcgcaagg agtgcgaacc ctggttttgc agatcatgct
      901 caacacaccc aaggcaacct ggggaattca ggacttggct aaagcgcgcg ccctgctgca
      961 gctggttcgg gagatcgagg acgagcattt ccatgtcttc ttacagccac gtggaggctt
     1021 ggactacttc tcgccggatg ctgaaaaggc gaaagcgcag ttggccgcag tgacggcact
     1081 catggatgac atcgagcctc atgatccgac aagtccaccg ctcattcacg tagtcagcta
     1141 ttccgaaggc tctcatcttg cggatcctgc ggttgtcaac gaaagcatcc aggtcacccg
     1201 ctacgctttg gagcagtacc gccgtctgcg tgccagaggt cacattgacg atatggcaaa
     1261 gcacccacag gtcactgaac gaacagcgga acttctctcc gaagcccgca ccgttttgga
     1321 gagcatcgaa cgcaccgttc ctgcccccta ttcggcagag gggctgtaca atattcttgt
     1381 ccagggtttc ttaccgatcc catacctttg ggaatgcaaa gacgaatttc ccaaagccac
     1441 ggcctggagc acccgactga tgagcgggtc ggtcaaggtg gtggacgccc agggacgagt
     1501 catcacggcc agagaacgca tgcgccttac gacggatcct ctatgaatca tggagacaga
     1561 ttacagcatt catgctgcga atcaagtcca aatggccaga aggggatgtt catgaaaaga
     1621 cttttgtttg tttttttgct cgtacctatt ctctgtggct gctacagaga gatcaagggt
     1681 gagaagccag gggagacgcc cagcaccgta tccccagtcg ctccgccatt actacctgtc
     1741 tccccgatgc cgccgcctgt gataccagtg ctgtggaaac ctttgcccaa gatgatcgag
     1801 gtgcgctaca gttttgcaac ggagattgtc aacggcagaa tctatgccat ggggggattg
     1861 tgtttcggca acggccttag aatgctgtct tcctgcgaag tgttggatcc tgagtcaaaa
     1921 acttggacac agttggggaa tatgcaggtc gcccgatctg attttgattc ggtgctcatt
     1981 gggagcagaa tttatgtcat ggggggagtg tatcgagacg aattccgagg ggacgttttt
     2041 ctttccagct gtgaagtgtt cgacacgaac acgggcacgt gggagtgtct gcccgatatg
     2101 aacgaagcaa gagccgcctt tcgggcggtt gcactgaacg ggaagatcta tgtcatggga
     2161 ggatgtaaca acctatcaga ggatctctcc agttgtgagg tgttcgatcc ggccaccggt
     2221 tcatggacaa tgctggcgag catgaatcag gcgcggattc tctttgagtc tgttgttctt
     2281 gatgggaaca tctacgtgtt gggagggagg accgaccgag gatcctattc agatgccctg
     2341 tccacctgtg aggtatacga taccaattcg aacacctggc aggcgcttcc agaaatgcga
     2401 agacctcacg acacctttca ggcggtcgta ctgaacaaca ggatctatgc gttgggagga
     2461 cattcccctt attgtgagtt tttcgatcct gaagaccgtt cgtggacgct gatatcaagc
     2521 atgacgcaag cgcgtcgttt cttccaggct gttgtgctga agaacaagat ctacgcgttt
     2581 ggtggggatt ctacaaacgg ttttgtctcc accgcagaag tctttgatcc gacggccaac
     2641 cagtggtttc ggctgacgga gatgagtgct ccacagggtc agtttcgggt agaagttctt
     2701 cggaacagca tttacgtcat aggcggtaga gcctgtgagt actgtgaacc tccgaagtga
     2761 cactcggttc ggaagaagcg cgtcatttac cgagcaagcg tccaagggag tgggcatagc
     2821 ccacatgcgt gcaggtgcta acctgctccg aagtgacgcc gccctacacc ggatgatcca
     2881 gtcgcttctt ggcatttcga aggcgctctc ggatgttcag cttctgtggg cagcgttctt
     2941 cgcaggctcc gcagtcggta caagcagcca catcgggctg aagtttgtga tattcctttc
     3001 tgctgcgagg ccattcaaag aatagacgac aaaggttctg atagagaacc atcagtttgg
     3061 caatgggaat gccggccgga cacggttcgc agtaaccgca gtcctgacag agcggcatct
     3121 tgggaaaaag cgactgccct gactcgatga acttctgacg ttcggtttca tccataggtt
     3181 cgaatctgtt ggcaaacttg tagttttcga ccatctctcc gacccgtttc ggaccgacca
     3241 aggcacagtc cactcccgag ttgctcagga caaaccgcag tgtgtctctc ggtttgatgt
     3301 cagacggttg gggttcctgg ccgtccttgc ctggccgaat cagccgaaat gcaacaccac
     3361 cgacattcgg tttcatgacc agcaccccaa cccccttgct ttgggcaagt ggaatgagtt
     3421 cgtattcagg ctccaatgtc atgaaattgt agcaggtcaa aatgacactg aactccccca
     3481 ttctggctaa atccatgagc atgggaacgt tatgtccgct gacgccgatg tagcggattt
     3541 tcccctgttg ttgtgccttc ttgactgcct caaaggctcc attgggagca aagaccaaat
     3601 cccatcgatc ctgtgccgag atgtcgtgaa gcatatagag atcgagatag tctgtctgca
     3661 gattgcggag cgaggtctcc agcgcctccg tggtttctga tgcggttcga aaatgcgact
     3721 tgctggccag gaagcactcc gaacgccgcg tcttcatgac ctctccgatt ttctcttcgc
     3781 tgtcttcgta agcgcgcgct gtgtcaatga agttgattcc caaatccaag gctttgtgga
     3841 tgatccgcac cgcacgccac ttgctctcct tgatgatcgg aatggtgccc aacccgatac
     3901 gtgtcaccac catgtctgtc tgtcccagtc gtctcttctc cacgcagatt ctctccttaa
     3961 gataagattc cgtaaactca gtcagtattg agactctcga tccttgtcaa aagcgggaag
     4021 ataacccggc tgcacttcca tggcagccct gtaaatgctg agagcctctt gcttccatcc
     4081 ctgtctttcc aggacacgcg cgagattgta ccgcgcttca cccgaaaacg gcagcagatc
     4141 acagagcacc cgcagatgcc tttcggcttc gggcaagttg cccatagtct cgtaaaggtc
     4201 ggccagactt tgacgccaaa gcgcgttcag gggatccacc tgtattgccg actggtacat
     4261 ctgcaatgcc cctgccgtat ctccttccag gaaaagcacc tgccccagaa gtcctcgata
     4321 atccgcgcga agcggacact tggcaattgc tgtatccagt gccagtcggg cgttttgtcg
     4381 ctcttgaact cctggggaag aaggcggatt tcgaaaggct cgaagaaaag tccgagcaat
     4441 ctcatagtca attcgactgt ccaaaggcga ccattcagac gcctcaacca gctctgtgag
     4501 aatggcagca cgacggcttt caggatctgc gaccaaggcc gctgctctgt tgaaatgaag
     4561 atccccgacc ttgtttcgga tgcttcgatg cagattgacg acaaaggcca tcagcaagag
     4621 tgaaagaaga aaggaacgag tcacaaccag cagatgcctg ccaacccaac tccgagagga
     4681 tgatcccgcc gttgcctgac tggacgtctg ctctttctcc cgtgcaggtc gccacgcgac
     4741 gagtgccccc aggatgatcc acacgagcgg acacaaaagc agatggacgc gaacatcagc
     4801 aaagcccgcc atgagcagcg agaccagcag agaaccgaga tagcccaagc gccagtcggg
     4861 tcccgctttg cgcatcttca ggcatctctc aacgaatgag gcgagtgcgg cgctgaacaa
     4921 aagaaccgca actgccagcc aagtccagcc catctcggcc tgaatgaccc ggtaggcgcc
     4981 accccagacc aagcgccact ggccaagttg tctatactgg ggaaggacgt cctcaagtgt
     5041 gccaagcccc gttcctgccg gcaggttgtc cacagaagcg cgaactatgg aaggttccgc
     5101 catttgaata cccagagata ggcccgggct tctcttgaga gtttccagca ccagacgtcc
     5161 aacaaaacct gccgttgaat agtgcgccag cgcaacgctg agaaagatgg tcaaggccag
     5221 cagaattgcc cttctggagc gatctgagct ggctccgatt cgggcagtac cgaacaccac
     5281 gagcgcggcc ggaagtgtga caaacagcag attcttcgga ttcccccagg ccacagtgaa
     5341 ggcaaacaag aaccagaggc acagggtgaa gacgatgccc acgcttgaga acatcttacg
     5401 gatgattcgg tgcagggtgg tttcacaacc tgttgcgtct gtctgactta ccggtgcgga
     5461 acaactgagg tcactcagta caagcagcaa cacgtagacc gttggaggga ggaatacgag
     5521 agtcagcagt ccggagcgcc ccaagagagc atccaacaga ttagagccgt gtggaaggct
     5581 gaacacaaat cggtgaaccc aacgattcag cggagaaagg ggaataagcg ttccgagcag
     5641 cccgatgaga gtcaggagca acatcatgcc aacaccagcg cgcttgccca cttctgtgat
     5701 cgcaacaaac acgagtgcat tggcaacaac gaggcgaagt gccgtgaaac tggtatgttg
     5761 acagatggag ccgtaggtcg ccagggaggc aaagaagaga atgacgagac tgaaggcagc
     5821 ccaagtgaca tgaggaatat ccactttctg atcgcgaatc ttgtgccaca ccagaacaca
     5881 gagtcgaaac acaaaccatc caccggtgat aagcagaaac aaggtatctg ccagcccacc
     5941 tcggtcctcg gggagcacaa cggcaagcag gatgacgagc cagatcatcg ttgtgcctcc
     6001 atgggcgtcc agacaccaaa gtccacacgc cgatatccgt ccagttccat caatacctga
     6061 tccatctgga agaggaacgg cagtggcagg tactgcagat accgctccga gacattcgaa
     6121 tagtctacct tgacaggcac ttgcctcaat ggaaagggtg cggaacgcaa atgaagcagg
     6181 aacagtggcg accatgtgaa gccagggatc gctgccgggg acataaaaca gtcgagttgg
     6241 ttgtattcaa gcggtcgggt ggagtagatt ctgtgggatg ccttggttgg gctgatgatc
     6301 ttccgccaga tcgcctgcat ccactcttgt cttgactgat atatctctga aggcagtgtg
     6361 gtttggatgc cttcccgcag cagcatgggt tcgtaccagg gggacgagag gaaattggct
     6421 cccacgacag ccacatcctg tcccataccg cagacgtatt tctgatacca gagtggat
//
LOCUS       DAOQUC010000031         6379 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_13428_length_6379_cov_3.148482, whole genome shotgun sequence.
ACCESSION   DAOQUC010000031 DAOQUC010000000
VERSION     DAOQUC010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6379)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6379)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6379
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13428_length_6379_cov_3.148482"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..409)
                     /locus_tag="PKH07_00985"
     CDS             complement(<1..409)
                     /locus_tag="PKH07_00985"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNT33554.1"
                     /translation="MRNDLRILIVDDEESIRNLLGDFISEHYPDVLLAENALKATEKL
                     QEYPVDVILTDLVMPEMTGRDLLKVVQERYPNTIVIVMTGHASVESAVDAMRLGAFDY
                     LVKPFNLSEAEITIERAGELLRLRKENARLIEDL"
     gene            complement(428..1438)
                     /locus_tag="PKH07_00990"
     CDS             complement(428..1438)
                     /locus_tag="PKH07_00990"
                     /inference="COORDINATES: protein motif:HMM:NF013269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HNT33555.1"
                     /translation="MSEIRRDPITTRWVAIAPEIIDDLSFFPSLREDDSASSCPFCPG
                     NEALTPASLWTDRMEAERPDEWHVRVFPNRTPILQTEVAENPQGKGVFDFMNGLGAHE
                     VVVHGREHTSHLSEFTRGQIAAGLRAYRDRMADLSGDRRLHYVMVHYDRGRCAGAALA
                     HPYSQIVATPKVPAAVEEELNGARSYYLIKERCVFCDVLTEELRMGTRMVVENEFYAA
                     FCPFASRSPYEVWLLPKMHQSDYTSQTDTQLEHLAETVQSIATKIDSVLGEPALHMAL
                     HSSPNLKAKSDNWRSVPQDFHWHMEFIPRVLERAAFEWGIGFHINPVAPEKAAQALRT
                     PH"
     gene            complement(1438..2238)
                     /locus_tag="PKH07_00995"
     CDS             complement(1438..2238)
                     /locus_tag="PKH07_00995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33556.1"
                     /translation="MGIAQMRAGVDLLREGWTMTKTKGCLIGCGSVAAIFFILVGFIG
                     YQLVQKTRHYAQEWSSLAKSYNETNEAFAFVSSPDGLLDPERFGLFLEVRNTLIAQTG
                     DEFRMFEAVFMAKNEQKDQSFGEVIQLARQIAQLPIKVGHKHVGILKENQMSFAEYKW
                     CTAGMLATIREGSKQGESFCWSLYDRLKTSEEKVGEINKSGSAPGGAAYQSVISSGNF
                     PKAVENVPLLLSHQSAISDTSEYLWLDCLIVGFAPERTAAGMPDNREP"
     gene            complement(2290..3600)
                     /locus_tag="PKH07_01000"
     CDS             complement(2290..3600)
                     /locus_tag="PKH07_01000"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HNT33557.1"
                     /translation="MAKSQKVNTNPPDHLDGSPTHQFVDAQVSKDYLLRVTKTFFRID
                     QILSTITDLKELLSLIMVESKSLVAAESSSLMLYDAQTDELYFEVALGPKGDEVKEIR
                     LRLDQGIAGYAAREGISVNVADASEDKRFLQGVDLKTGYQTRSIVAVPMKRKGKLIGI
                     IEVLNKKDGGSFSDEDVKILEVLAHLAAIAIENARLFEANVRSERMAALGEAIAGMAH
                     CVKNILTGMQGGSSLVEMAITNQKYEILPQAWDILKRSSARVSSLVQNMLTYSKDRVP
                     IRKPTCINRLVQEVCASQTMRAHEEHIALEFEPCHEDLVANVDSQALERCVQNLVVNA
                     IDAIREALAERDLQGHVLVKTRLSADRTEFFIEVSDNGCGIPKEALPRIWQAFYSTKG
                     SSGTGLGLSVTAKIVQEHGGRIDVKSELEKGTTFSIILPAESLE"
     gene            complement(3606..4691)
                     /locus_tag="PKH07_01005"
     CDS             complement(3606..4691)
                     /locus_tag="PKH07_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008437046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HNT33558.1"
                     /translation="MNLNEMLDAMFFQNAADLYLSAGAPPTLRERHQLSPITDTPLEP
                     EETEAIAFELMDDAQRAEFTRTGEMNLAYTVLERGRYRVNAFRQKGAVSLVIRLIKFD
                     IPSIEDLTLPPILKELIMRPRGLVLVTGATGSGKSTTLASMIDYRNRTLAGHIITLED
                     PIEYVHNHQKCVINQREIGLDTISYEIGMKNVLRQAPDVILVGEIRDRDSMEAVMNYS
                     ETGHLVLSTLHSINANQTLERIMQFFPSDIEEKVFQQLSMTLEAVISQRLVPRKDGSG
                     VVPAVEVMIASPRIRELVRKGEIQDMKKTIEASRQDGMQTFDQCLFDLINSGVVTKED
                     GLHAADSPGDLSLKLQGLTSGTANIMA"
     gene            complement(4859..>6379)
                     /locus_tag="PKH07_01010"
     CDS             complement(4859..>6379)
                     /locus_tag="PKH07_01010"
                     /inference="COORDINATES: protein motif:HMM:NF012892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HNT33559.1"
                     /translation="NLFQDQGDWQVAGDPTEGALLVLAKKGDRLLNVEGTRVKEFPFD
                     SIRKMMSSVFMTDEGKGLFVLTKGATETLLDLCTHEAVGDETVAFDQDRRERVAAEML
                     AMAERGLRVLGLAYKQVAEVPDSIHNAESDLVFLGLVGLVDPANEAIRGEIDACHRAG
                     IQVKMLTGDHRTTAQAIGSFLGLATDGEEHVVEGKDFETYEGDNLDSMINQATVFSRV
                     APETKLRIVEALQKSGHVVAMTGDGVNDGPALMRSDIGIAMGKRGTEVAKQAADMILQ
                     DDDFSSIVAAVREGRVIYSNITKFIHYLFACNIGEILIVFLGSLFSRVLPLFPLQILW
                     VNFVTDVFPALALSQEKADEDVMVQPPRPPKEKLLNKGRLITVLWQGLLLTSLCLGVL
                     FWALGRSDKPANYAQTMTFTAMVMMQLGHLFHCRNPKRTVFRQNLASNWYILFAVISS
                     VLLQLIVLYTPLFQEIFRTVPLHASDWEIIFSLVATRVLVNEVVRLVKKRLKVKNA"
BASE COUNT         1465 a         1827 c         1586 g         1501 t
ORIGIN      
        1 gcaaatcctc gatcaatcga gcattttcct tgcgcagtcg gagcagttcc ccagcgcgtt
       61 caatggtgat ctcggcttca gaaagattga acggcttgac cagatagtca aacgctccca
      121 aacgcatggc atccacggcg gactccacgc tggcatgacc ggtcatgaca ataacgatgg
      181 tgtttggata ccgctcttga accaccttca gcagatctct gcccgtcatc tcgggcatca
      241 ccaaatctgt caggatcacg tcaacgggat actcttggag tttctctgtc gctttcagtg
      301 cgttctcagc gagaaggaca tccggatagt gctcactgat aaagtcccca agcaggttgc
      361 gaatagactc ttcgtcgtcc acaatgagta ttcgcaagtc gtttctcatg cgtgtcagtc
      421 cttcccgcta gtgcggtgtt cgtagcgcct gcgccgcttt ctcgggcgct actgggttga
      481 tgtgaaatcc aattccccat tcgaaggcag cacgttccag cactcggggg atgaattcca
      541 tatgccagtg gaaatcctga ggtacgctac gccagttgtc cgatttggct ttcaggttcg
      601 gactgctgtg caaagccata tgcagggcag gttcgcccag tacggagtca atcttcgtgg
      661 caatggactg aaccgtctct gccagatgct ccaactgggt atccgtctgg gacgtgtagt
      721 ccgactgatg catcttggga agcagccaaa cctcataggg gctccttgag gcgaatgggc
      781 agaaggccgc atagaactca ttctccacta ccatgcgtgt acccattctc aattcctcgg
      841 tcaacacatc acagaacacg caacgctcct tgatcaggta gtaactgcgc gctccgttga
      901 gttcctcttc cacggcagcg ggcaccttgg gcgtggcgac aatttgcgaa taaggatgcg
      961 ccagcgccgc acccgcgcac cttcctctat catagtgaac cataacataa tgtaggcgtc
     1021 ggtctccaga taaatctgcc atccggtctc gataggcacg gagccccgcc gcgatctgcc
     1081 cgcgcgtgaa ctctgagaga tgtgacgtgt gctcccgacc atgaaccacg acctcatggg
     1141 cacccaagcc gttcatgaag tcaaagacgc cctttccctg cgggttctca gcgacttccg
     1201 tctgcaggat aggcgtccga tttgggaaaa ctcttacatg ccactcgtcc ggtctttcag
     1261 cctccatcct gtctgtccac aacgatgcag gcgtcagagc ctcatttccg gggcaaaaag
     1321 ggcaggatga cgcggaatca tcttcacgca gactcgggaa gaagctcaaa tcgtctataa
     1381 tctcaggagc tattgccacc catcgtgtgg ttatcggatc tcgacgtatc tcagacatta
     1441 cggctcccgg ttgtctggca tgcccgcagc ggttctctcg ggagcaaatc ccacgataag
     1501 gcagtcaagc caaagatact cgcttgtgtc gctgattgcg ctttggtgtg acagcagcag
     1561 tggaacgttc tcaaccgctt tggggaagtt cccagaactg ataacgctct ggtacgctgc
     1621 tccaccgggg gcgcttcccg acttgttgat ctcaccgact ttctcctcgg aggtcttcag
     1681 ccgatcatac agactccaac aaaacgactc cccctgcttg gaaccctctc gaatggtcgc
     1741 cagcatacct gcagtacacc acttgtattc ggcaaatgac atttgattct ctttgaggat
     1801 gccaacgtgt ttgtgcccga ccttgatggg caactgtgcg atctggcgcg ccagttggat
     1861 gacttctcca aaagactgat ccttctgttc gttcttggcc atgaagacag cctcaaacat
     1921 cctgaactca tctcccgtct gggcgatcaa cgtgtttcgc acttccaaga acagcccgaa
     1981 gcgctccgga tcgagcaaac catccggaga agagacgaaa gcaaaggcct cattggtctc
     2041 gttgtacgac ttggccagcg aagaccactc ctgagcatag tgacgagtct tctgcaccaa
     2101 ctgataccca atgaaaccaa ccaggatgaa gaagatggcg gcgaccgacc cgcatccgat
     2161 gagacaaccc tttgtcttgg tcatagtcca tccctctctg agcaggtcaa cacctgcacg
     2221 catctgggca atgcccattc ctttggacgc ttgctcaaca aatgagctgc ccgccagaaa
     2281 ttctgctttc tattccaaag actctgccgg caggatgatg gaaaaggtcg ttcctttctc
     2341 caactccgat ttcacgtcaa tgcgaccacc gtgttcctga acgatcttgg ctgtcacaga
     2401 aagccccagc ccggtgccac ttgagccttt tgtactgtag aaggcctgcc agattctggg
     2461 gagcgcttcc ttgggtattc cacagccatt gtcggacacc tcaatgaaga actctgtcct
     2521 gtccgcggag agtcttgttt tgacaagcac atgaccctgc aaatcccgtt cagcaagagc
     2581 ttccctgatg gcgtctatgg cattcacgac aagattctga acgcaccgct caagcgcttg
     2641 agaatccacg ttggcgacaa gatcctcatg acagggctca aactcaagag caatatgctc
     2701 ttcatgggct ctcatagtct gactggcgca gacttcctgc acaagccggt tgatgcatgt
     2761 tggctttcgg atcggcaccc ggtccttgga gtaggtcagc atgttctgaa cgagacttga
     2821 aacccgagca ctgcttcgct tgagaatgtc ccacgcctgg ggaagtatct catatttttg
     2881 attggtgatt gccatttcca ccagagagga acccccctgc atcccagtca ggatattctt
     2941 gacgcagtgc gccatcccag cgattgcctc tccgagagcg gccatgcgtt ctgagcgaac
     3001 attcgcctcg aacagccgcg cattctcaat ggcaatggcc gccagatgag caagcacctc
     3061 aaggatcttg acgtcctcat cggagaaaga gcccccgtcc ttcttgttca gcacttcgat
     3121 gatcccgatg agtttcccct tgcgtttcat cggaacggca acaatagatc gcgtctgata
     3181 gccagtcttg agatccactc cctgtagaaa tcgcttatct tcactggcgt ctgccacatt
     3241 gacagagatc ccttcgcgcg cagcgtatcc cgcgatgcct tgatccaacc gcaggcggat
     3301 ctctttgact tcatctccct tgggaccaag agcgacctca aagtacagtt catccgtctg
     3361 agcgtcatag agcatcagac tgcttgactc agcagcgacc agacttttgc tctcaaccat
     3421 gatcaggctt agcagttcct tgagatctgt gattgtggaa aggatctggt ctattcgaaa
     3481 gaacgttttc gtaacgcgaa gaagatagtc cttggacact tgggcatcca caaactgatg
     3541 cgtcggagaa ccgtccagat gatccggggg atttgtgttg actttctgcg atttggccat
     3601 tgtcctcaag ccatgatatt ggcggtaccg gaagtcagcc cctgaagttt cagactcaag
     3661 tcgccaggac tgtctgcggc gtgcagaccg tcttccttgg taaccacacc actgttgatt
     3721 agatcgaaca gacattgatc aaaggtctgc atcccatcct gacggctggc ttcgatggtc
     3781 tttttcatat cctgaatctc accttttcga accaattctc gaatgcgagg cgaggcaatc
     3841 atgacctcca ccgccggtac aacaccggaa ccgtcctttc tgggtaccaa gcgttgcgag
     3901 atgaccgctt ccagcgtcat ggacagttgc tggaagacct tctcttcgat atccgaaggg
     3961 aagaactgca tgatacgttc caaggtctgg ttggcgttga ttgagtgcag tgtggacaac
     4021 accaagtgac ccgtttcaga gtagttcatg acggcttcca tgctgtccct gtctcgaatc
     4081 tcacccacca gaatcacgtc aggagcttgg cgcagcacat tcttcatgcc gatttcatag
     4141 gagatggtgt ccaagccaat ttcacgctgg ttgataacgc acttctggtg attgtgcaca
     4201 tactcaatag gatcctcaag cgtgatgata tgaccggcca gggttcgatt gcggtagtca
     4261 atcatggacg ccagtgttgt ggatttcccg ctaccggtcg ctcccgtcac caggacgagt
     4321 cctcgaggtc tcatgatcag ttctttgaga atcggcggga gtgtcagatc ttcaatactg
     4381 ggaatatcga acttgattag acggataacc agactcaccg ctcccttttg tcgaaaggcg
     4441 ttcacacgat accgtccgcg ctccagcacc gtgtatgcca ggttcatctc ccctgttcga
     4501 gtgaactcgg cgcgttgtgc gtcgtccatg agttcaaagg caatggcctc cgtttcctcg
     4561 ggttccagtg gcgtgtcggt gatcggggaa agttgatgcc gctcccgcag agtcggagga
     4621 gcaccggcag agagataaag atccgcggca ttctggaaga acatggcgtc gagcatttcg
     4681 ttgaggttca tggcgaaggt tcccctatta tctgtgaatc aattatcata tatcatagtg
     4741 tccaaagtac accgatggga gcaagagggt caagggaaaa ctctgcgctg ccaatcgatg
     4801 cgttgtgttc tacaccccat ttgcagcccg acatgctcac ccgcataccc tcaggcgtct
     4861 aggcgttttt taccttcaag cgcttcttaa caagtcttac cacttcattg accaacacgc
     4921 gagttgccac caaagagaag atgatctccc agtcggaagc gtgcaaaggc accgtgcgaa
     4981 agatctcctg gaacaacggc gtgtagagaa cgatcagctg aagcagcacc gaactgatta
     5041 cggcaaaaag gatgtaccag ttcgaggcca gattctgtcg aaagacggtt cttttcggat
     5101 tgcggcaatg aaacagatgc cccaattgca tcatcaccat ggcagtgaag gtcatcgttt
     5161 gggcgtagtt cgcaggtttg tccgatctac ccaaagccca gaagagaacg ccgagacaca
     5221 aactcgtcag gagcagtccc tgccagagga ccgtgatgag cctccctttg ttgagcagtt
     5281 tttcctttgg cggtcgtggc ggctgaacca tcacatcctc atcagccttc tcctgagaca
     5341 atgccagggc tggaaaaaca tcggtcacaa agttcaccca gagaatctgc aggggaaaca
     5401 agggcaagac tctcgaaaac agcgagccga gaaacacgat cagaatctca ccgatattgc
     5461 aggcaaacag gtagtggatg aacttggtga tattggaata gatgacccgg ccttctcgaa
     5521 ccgccgcgac gatggatgaa aaatcgtcgt cctgaagaat catgtcggcc gcctgtttgg
     5581 cgacttccgt ccctctctta cccatggcaa tcccgatgtc cgagcgcatc aatgctggtc
     5641 catcgttgac tccatcgccg gtcatcgcga caacatggcc ggacttctgt aacgcctcga
     5701 caatccggag tttggtttcc ggagctacgc gagagaagac ggttgcctga ttgatcatgc
     5761 tatcgagatt gtctccttcg tatgtctcga aatccttgcc ctcgaccacg tgctcctcgc
     5821 cgtcggtcgc cagcccaaga aatgagccaa tcgcctgagc cgtcgtgcgg tgatcaccgg
     5881 tcagcatctt gacctgaatg ccagcacgat gacaggcgtc aatctcgcct cgaatggctt
     5941 cgttggccgg atccaccaga ccgacaagac cgagaaacac caaatcgctc tctgcgttgt
     6001 ggatggaatc cggcacttcc gccacttgtt tgtatgccag accaagaacc cgaagacccc
     6061 gttcggccat ggccaacatt tctgctgcca ctctctcgcg acggtcttgg tcaaacgcaa
     6121 cggtctcatc gccaaccgct tcgtgtgtac acagatccag aagtgtctcc gttgccccct
     6181 ttgtcagaac aaagagacct ttgccctcgt cggtcatgaa gaccgaggac atcatctttc
     6241 gtatggaatc aaacggaaac tccttgacac gcgttccttc cacattcaga aggcggtccc
     6301 ccttttttgc aagaaccaga agcgcgccct cagtggggtc gcccgccacc tgccagtcac
     6361 cttgatcctg aaagaggtt
//
LOCUS       DAOQUC010000032         6348 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_13519_length_6348_cov_3.224853, whole genome shotgun sequence.
ACCESSION   DAOQUC010000032 DAOQUC010000000
VERSION     DAOQUC010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6348)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6348)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6348
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13519_length_6348_cov_3.224853"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..589
                     /locus_tag="PKH07_01015"
     CDS             <1..589
                     /locus_tag="PKH07_01015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33560.1"
                     /translation="EYARDYLRATFGYYGAPEPMIIKSTSYSPSTSYHGLSNTGQDLL
                     PPDIEGAFEPVVVDTPQQVEIFALATDDLNVNSVQATIQKPDGAVVDMTLQLTDPVAR
                     KYTAELDSSVLSVLGAYNITIVALDDARNVSEPHVSHLTVFSPADLNGDMVVNAEDLV
                     LLIGEMGQSYSPLEIVRDGTMNYLDLLAVARYWQR"
     gene            665..2527
                     /locus_tag="PKH07_01020"
     CDS             665..2527
                     /locus_tag="PKH07_01020"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNT33561.1"
                     /translation="MIRSKLTLFTVLVVVVCVGCTAQERPLRPGQTKGIFRGTWWGYY
                     ERGRSYADAGMMEEAIKDFQAAIRLRAEDTHYARTYGVHFTAYYPNRELGCCYFAQGK
                     VDDAKKALDASQRSAPTHKTDHFLELITQKQLLATRDDAEPPQLALLWPPHQSDIFIN
                     QYDAVLEGELQDDLRVTQMAINDARIQIQPGERTKFSHPIKNLQEGPNPVQLRAVDLT
                     GKETAQEHVVVVDVGGPVVNLQAPVVETTATGKKVTVAGTIEDDNLLSSVKIGDRDLG
                     GDHQKSVAFTETFEIGLDVSEIKILASDLAGNQSSGVVSLAPTPTPAGPTDTVPPTLS
                     LPPEKLVFFTDMATLEIRAEDNQSVTSIEIAGKPVAVVPSAQAFTVHRIPLQEGENVI
                     PVKVADAAGNITEKDVKVTKYSQAIKMVDVRMALGMTAQEEEGPKIEVAPRVPDKLAS
                     AIVARDRFQVSVSETAAKDALLQSKVHETPDSIEVVVRMVDPETGTVMGIFDAFEKDK
                     SEATLKYLADIVAEKMENAIPVIEGTVAKVAGSNLVLTFGVDAALPKYARCILYRDGE
                     EIKDPNTGAVVGSLTEEVAELVVTNVQQRMVQAKVKQMLVADATLAVGDKVITK"
     gene            2703..3239
                     /locus_tag="PKH07_01025"
     CDS             2703..3239
                     /locus_tag="PKH07_01025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33562.1"
                     /translation="MSVEVVENKSLTDASEDGLSGWLRLEGLGIREVHLENLGKQNTA
                     GLANAPQRLHLRYENDGIYKVPVGAYRIAAAQLAESATADLIYSLQQASARSITARVV
                     PDGTETLRIGGPLTERFSYATGSFGADIVLSYAGCFNESELRFSRESLSGRNETLPMW
                     EVRDASGMLVRSGRFSFG"
     gene            complement(3462..4892)
                     /locus_tag="PKH07_01030"
     CDS             complement(3462..4892)
                     /locus_tag="PKH07_01030"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNT33563.1"
                     /translation="MTVCFITSKIRSCCSIALVAFMVSGICSCVYSRFLLRHAEIEWM
                     EQQNIHTMNVFWYWCEQMRLDPMKALFDDDLLAQTWHAHHLHPNVPVILMALFRGRQA
                     GSVVLADASPLPPSRLFVDGGIIPSVLREEDIPRTRLLPLLLGLTSVLLLLVWVFQEA
                     GLRQALWTGALVLMMPQFLARLCSVSTGSAVVVLWLVLLWTFRRAKDTLGCRIAIGTV
                     LGVGLGTKATIVLAPICYMLYVLLELWKRGTLTARPLLKSGRFWLLVVVVGLSVHLVL
                     WPWLWRNPVMRTVEHLAANRLEAESTVEIFGFTLERPPFFYPFWFLVTTTPIPILLFM
                     FVGIWEVLKRLKSADLDASRHSWVRLHLVSTIVLLSLFLLPLRINLNGAEFFLPLYPS
                     MAVLASEGLLSLCQRVRLLIFQRAVAMEALALIILLLSVSLDFRFGESVFHYRNIAGT
                     TMREPSLHAFPRTAAAMYPLHVSRAP"
     gene            complement(5098..>6348)
                     /locus_tag="PKH07_01035"
     CDS             complement(5098..>6348)
                     /locus_tag="PKH07_01035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNT33564.1"
                     /translation="KVVKCLGEADRQGVQILRRPRTLIETAYNHLARNTIVQQIGLAL
                     TDWAASADPLLAVQSREGLSQIATRISPFLFRSNVFGKLWMFLYVPSGSHYVSVGSSD
                     ALEVQPAEHDGVIQLRFQNGQAFSLEPTAIKRLYSDDRHIVATLEVDDLHYGALVLEA
                     RHTKSSLADEDRTMVADVASKIEQTLSNIASHLLRSEKQKADISVALSTSMGHDLTNM
                     IATSKWDIRTVQMYLDRVLPKLGDDRQAKIFGQSLQGLLNNMQLLQEVVDLYRSLGLT
                     KRPQYELTNMNHLVDDAVNLFQFSTSRNVVLTTEHEKGLAEVAVEPRLLKVALFNLLS
                     NAGQACAKRSIQIGQSEEKYFGEIHVQTLTAKDNHIAIEIRDNGTGFLDKEGRRLPGD
                     QIHRTFRSGYTTKTGRVAGGLGLS"
BASE COUNT         1585 a         1622 c         1762 g         1379 t
ORIGIN      
        1 tgaatacgcc agagactatt tgagagcgac ctttggctac tatggtgcac cggagccaat
       61 gattatcaag agcaccagtt acagtccaag cacaagttac catgggttgt ccaatacagg
      121 tcaggacttg ttgccgcccg acatcgaggg tgcatttgag cctgtagttg ttgacacacc
      181 acagcaggtg gaaatcttcg cgttggctac agacgatcta aatgtgaatt cagtgcaggc
      241 caccatacag aaacccgatg gtgcagtcgt ggatatgaca ctacagctca cagatcctgt
      301 tgcgcgcaaa tatacggccg aattggattc gagtgttctg agcgtgctgg gtgcttacaa
      361 catcacgatc gttgcccttg atgatgcgag aaacgtctct gaaccacacg tgtcgcattt
      421 gacggtgttc tcacccgccg acctgaatgg tgacatggtc gtcaatgcag aagatctggt
      481 tctgctcatc ggagagatgg gacagagtta cagtcccctt gaaatcgttc gggatggaac
      541 catgaactac ctggatctgc tggcggtcgc caggtattgg cagagatagc tcgcatggtc
      601 aacgcagaag agacgtgcta gactttccgc gtagggagtt agtgatgttc aggaggttgg
      661 tatcatgatt cgctccaaac tgactttgtt cacagtgctg gtggtcgtcg tctgtgtcgg
      721 gtgtacggca caggaaaggc cactgaggcc aggacagacc aagggaatct ttcggggcac
      781 ctggtgggga tattacgaac gtggccggtc gtacgcggat gccgggatga tggaagaggc
      841 gatcaaagat tttcaggctg ccattcgtct ccgcgccgag gatacacact acgcgagaac
      901 ctacggagtc cacttcacgg cgtactatcc caaccgagag ttgggttgct gctatttcgc
      961 tcaagggaag gttgacgatg cgaaaaaggc attggacgcc tctcagagaa gtgcgccaac
     1021 acacaagacg gatcattttc tggagctgat cacgcagaag cagttgcttg ccactcggga
     1081 tgacgcggag ccgccgcagt tggcattgct ctggcctcca catcagagtg atatcttcat
     1141 caaccaatac gacgccgttc tcgaggggga gttgcaggat gaccttcggg tcacccagat
     1201 ggcaatcaac gatgctcgta ttcagattca gcctggagaa cgaaccaagt tctctcatcc
     1261 catcaagaat ttgcaggaag gtcccaatcc ggttcaactg cgcgcagtgg acttgaccgg
     1321 caaggaaacc gctcaggagc atgtggtggt cgtggacgtt ggcggacctg tggtgaacct
     1381 gcaggcgccg gttgtggaaa ccacggcaac aggcaagaag gtgacagtcg ctggcacaat
     1441 tgaggatgac aacctgttgt cttcagtcaa gattggggat agggacttgg gtggagacca
     1501 tcagaagagt gtagcattca ctgagacttt cgagattggt ttggacgtaa gcgaaatcaa
     1561 aatcctggct tccgatctgg caggcaacca gtcctcaggc gttgtttcat tggctcccac
     1621 accaacgcca gccggaccta cggatacggt gcctccaact ctttctttgc cacccgagaa
     1681 actcgtattc ttcacagaca tggcaaccct tgagattcga gcggaggaca accaatcagt
     1741 cacgtctatc gaaatcgctg gaaagccagt agccgttgtc ccatcggcac aggccttcac
     1801 cgtacaccga attcctttgc aggaagggga gaatgtgatt cccgtgaaag tggcagacgc
     1861 cgcgggaaac atcacagaga aggacgttaa ggtcactaaa tactcgcaag ccatcaagat
     1921 ggtggatgtt cgaatggctc ttggcatgac ggcacaggaa gaggaagggc cgaagatcga
     1981 agtggcgccg cgtgtgcctg acaaattggc ttccgctatc gtggcgcgtg accgttttca
     2041 ggtcagcgtt tccgagacag ccgccaaaga tgccctgctc cagtccaaag tccatgagac
     2101 acctgactcc attgaagtcg tggtgcgaat ggtggatccc gagaccggta cggtcatggg
     2161 tattttcgac gcatttgaga aagataaatc agaagccacc cttaagtacc tcgcagacat
     2221 cgtcgcggaa aagatggaaa acgccattcc cgtcatcgag ggaacggttg ccaaagtcgc
     2281 gggctctaat cttgtgctga cgttcggagt ggatgcagca ctgcccaagt acgcccgatg
     2341 catcctctat cgggacggtg aagagatcaa ggatccgaac accggcgcgg tcgtgggatc
     2401 tttgactgag gaagtcgcgg aattggtggt gaccaatgtt cagcaaagaa tggttcaggc
     2461 aaaagtgaaa cagatgctgg tcgcggacgc caccttggct gtgggagata aggtcatcac
     2521 caagtagagt gaatgcgaat actaaggaac aatcagtcaa cgccatgagg gcgggattct
     2581 tggaagccca cagtcctgtc ctttctcgcg aaagacttcc tacgagacag aggttctgtc
     2641 ctgtcgtctt gctttgtctg tgtctggggt acttgttctt cgcaggcatg gcatcggctc
     2701 agatgtcagt tgaagttgtt gagaacaagt ccttaacaga tgcatccgaa gatggtctgt
     2761 ctggttggct tcggcttgaa ggattgggca tccgcgaagt gcatctggag aatcttggca
     2821 aacagaacac agctggtttg gcgaacgctc cccagaggct ccatcttcgc tacgagaatg
     2881 atggtattta caaggttcca gtgggagctt accgaatcgc tgccgctcaa ctggctgaga
     2941 gcgcaacagc ggatctcatc tactctttgc agcaggcctc agcaagaagc atcaccgctc
     3001 gcgtcgtacc cgatgggaca gagaccttgc ggatcggagg gccgctgact gagagattca
     3061 gttatgcaac aggctccttt ggtgcggaca ttgttctcag ctatgccggc tgcttcaatg
     3121 aatctgagtt gcgtttttca cgagagagcc tctcagggag aaacgagact ctgcccatgt
     3181 gggaagtgcg agatgcttcg ggaatgcttg tgcgttccgg cagattcagt tttggctgag
     3241 gggggacgtg cagggcttcc gttggaatgc ccgcttctgc ttcgggaaca tttcgcgcca
     3301 gtatcgttgt gccagctggc gagttggtga agcctcagtc atacagtttt gaattctatc
     3361 ctcccaaatg gagtgcttgg gtgtctatca tgcccggtct gcttcttgtg ctgaacctga
     3421 gcatactcct cggagcgatt tacgttgtct ggcgacaagc ctcacggcgc tcttgagacg
     3481 tgcaatggat acatcgccgc ggcagtccgt ggaaaggcgt ggagactggg ttcacgcata
     3541 gtcgttcctg caatgttcct gtagtgaaag acgctctcgc cgaatcgaaa gtccaaagac
     3601 accgacaaca gcaggatgat cagagcaagg gcctccatag caactgccct ctggaaaatg
     3661 agcagccgaa ctctctgaca aagggagaga agcccctccg atgccagcac cgccatggac
     3721 ggatacagtg gcaagaagaa ctcagcgccg ttcaggttga tccgcaacgg aagaaggaag
     3781 agagagagaa gcacaattgt ggaaaccagg tggagtcgaa cccatgaatg tcttgaggcg
     3841 tccaggtcag ccgacttaag ccgcttcaac acttcccaga ttccgacgaa cataaacaga
     3901 agaatgggaa tgggggtggt cgtcacaaga aaccaaaacg gatagaagaa cggtggacgc
     3961 tccaaggtga atccaaagat ctccactgtg ctctctgcct caagtctgtt ggcagccagg
     4021 tgctccaccg ttcgcatgac gggattgcgc caaagccaag gccagagcac aaggtggaca
     4081 ctcaacccca caacaaccac cagcagccag aagcggcctg acttcagaag cggtctcgcg
     4141 gtcaaggttc ccctcttcca caactccagc aacacataca gcatataaca gatgggcgcg
     4201 agaacaatgg tcgctttggt tccaagtccg acgccaagga cagtgccaat ggcaattctg
     4261 cagcccagag tgtctttcgc tctgcgaaaa gtccacagca gaacaagcca gagaaccaca
     4321 acagctgaac cggtcgaaac cgaacagaga cgcgccaaga attgaggcat catcagaaca
     4381 agagcacccg tccagagagc ctgacgcagt cctgcttcct ggaaaaccca gaccaacagg
     4441 agaagaacgc tcgtcaaccc cagcagaaga ggcagcaacc gagttcgagg aatgtcctct
     4501 tcccgcagga cggaagggat tatgccccca tcaacaaaaa gccgtgacgg aggcaatggg
     4561 gaagcatccg ccagtacaac cgagcccgct tgtctacctc gaaacagagc cataaggatg
     4621 acaggaacgt tgggatgaag atggtgggca tgccaagtct gggcgagcag atcatcatcg
     4681 aacaacgcct tcattggatc aagacgcatc tgttcgcacc agtaccagaa aacattcatg
     4741 gtgtgtatgt tctgctgctc catccattca atttcagcat gtcgcagcag aaagcggctg
     4801 tagacacaag aacagatgcc cgacaccatg aaagcaacaa gggcgatgga gcagcatgat
     4861 cgaatctttg atgtgatgaa acagactgtc atgcgatctt tctgtgtgcc tcttcatgtt
     4921 gactgcatct tcagtattcc agcagcctca agcactctct gtttgttgac tcaccgcagc
     4981 gttgagttcg tcgggattgt ctctaatcgg gagaagaatc cgcaccaaag cgccgtgtgg
     5041 ttccagattc tcggcttcaa tgtcgccggc atgcaagtcc cgcacgatgg tctgaattca
     5101 actgagtccg agacctcccg ctacacggcc tgtcttggtg gtgtatcccg agcggaaagt
     5161 ccggtgaatc tgatcgccgg ggaggcgtcg cccttccttg tcaagaaagc cggttccgtt
     5221 atcacgaatc tcgatggcga tgtgattgtc ctttgcggta agagtctgaa catgaatttc
     5281 gccgaaatac ttctcttcag actgaccaat ctgaatgctt cgtttggcgc atgcctgacc
     5341 cgcgttggaa agcaggttaa acagcgccac tttcaacaac ctcggttcga cagccacctc
     5401 agcaagacct ttttcatgct ctgtggtcaa cacgacattg cgagaggtcg agaattgaaa
     5461 aagattcaca gcatcatcca ccagatggtt catgttcgtc agctcatact ggggacgttt
     5521 ggtcaagccc agagagcggt acagatcgac cacttcctgc aacagttgca tgttgttcaa
     5581 cagcccctga agcgactgcc cgaaaatctt tgcctgccga tcgtcaccaa gtttcgggag
     5641 tacgcggtcg aggtacatct gcaccgttcg aatatcccat tttgaggtgg caatcatatt
     5701 ggtcagatcg tgtcccatac tcgtcgagag tgccacgctg atatcggctt tctgcttctc
     5761 gctgcgaaga aggtgcgagg cgatattgga caaagtctgc tcgatcttgc tcgccacatc
     5821 tgccaccatg gttctgtctt cgtcagcgag ggatgacttg gtgtgtcttg cctcaagcac
     5881 caaggcaccg tagtgcaggt catccacttc aagtgttgcg actatatggc gatcgtcgga
     5941 atagaggcgc ttgatggcag taggctcaag actaaatgct tgtccgttct gaaaacgaag
     6001 ttggatgact ccatcgtgct cagcaggttg aacttccaga gcgtccgaac tgccaacaga
     6061 aacataatga gagcctgaag ggacatagag gaacatccag agtttgccaa agacattgga
     6121 gcgaaagaga aacggggaaa tccttgtggc tatctgacta agtccttcac ggctctgaac
     6181 tgccaggagt gggtcggctg atgcagccca gtcggtaaga gccagcccaa tctgttgcac
     6241 aatggtgtta cgtgcaagat gattgtaggc ggtttcgatc aaggtgcggg gacggcgcag
     6301 aatctgaacg ccctgacggt cggcctctcc caggcacttg acaacctt
//
LOCUS       DAOQUC010000033         6318 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_13613_length_6318_cov_3.136197, whole genome shotgun sequence.
ACCESSION   DAOQUC010000033 DAOQUC010000000
VERSION     DAOQUC010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6318
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13613_length_6318_cov_3.136197"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..866
                     /locus_tag="PKH07_01040"
     CDS             <1..866
                     /locus_tag="PKH07_01040"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNT33565.1"
                     /translation="NLELAQSNDVSDSSESAWAPRQSNTPVSENDAVERAKLVIHGLL
                     LGVSESIESLLTDSTKHTDSIEQHKVAVKKAMTVAGIKELERLILDELEKMRSSNDQY
                     RGQLSEANGRLKIQQDVLEKLQADVRMDFLTKLSNRRAFDTRFVEETERARRYGHQLS
                     LILMDADRFKAINDVYGHVAGDRILRGVAKVIEGETRSSDFVARYGGEEFVLLLPETS
                     MQQASAVAEKIRKKIEQTKFWVDTRNIKVTVSLGVAQFDAQNDTRESFLKRADAAMYR
                     AKEAGRNRVEC"
     gene            860..2140
                     /locus_tag="PKH07_01045"
     CDS             860..2140
                     /locus_tag="PKH07_01045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33566.1"
                     /translation="MLKSHNPYRIMPLETDIRILHPLIERLVELANLPSEDRKKRLWA
                     DHQALLPTRKIPVCVYYEGIPGSQWDTMFGSDHLQTEDSLARSIEFDLKRRIWMAENV
                     PDDHIVWPSVTIHAPMKIVQNWGVSIQWSSSIHPLGARGYKPPFEEQIDLSQLTQPVY
                     RFDTEATQTLKDKATELTQGMLDVFVHYASMGYAPFDVATSMRGMTNLMMDVVECPDQ
                     VTGLMAFLTDAEIAHETWREQNNCINVFPDRTQTYQQVGFRVHCAYLPAGFGDRKARL
                     EDEWTYLSQQTSAGLGPEQYRDFVQPFNVALASLFSQETVYYHGCECLDQKLAVLAST
                     PNLRRFHISPWSSVAKARDVFQGKMVLEVHDHPGRVFFGATREEIRTNLRCLVQQAQG
                     HPMDLNISDIHSFGGKPEMLSLWAEIAQDVASGN"
     gene            complement(2142..3605)
                     /locus_tag="PKH07_01050"
     CDS             complement(2142..3605)
                     /locus_tag="PKH07_01050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33567.1"
                     /translation="MLHFFDDAHLAEQEGLTRVPGPVEKIGPVIDPIASDGPFVASFA
                     SSLVQLDGGRWRFYYTCQPSSSSQHRLTWRLGMGVAESEDLLHWTRPNLGQVQFEGKD
                     TNLLKIEGIPDPNNKVNYGQPQVFRLSENEWRMYFWLHGGYLRYAIAHSKDGLSWRVD
                     DVNQAAIFHPSELQPYGFGMGCDIEAIAGKPPFEGVSAEQLRHKRRLRSNDATFVYFN
                     EPLKRFEMYSVWLMYNPKDGPRHIAYDNAPFSLRTVHRRISENGIHWSDPELLITPDS
                     DDPTDQQFYFLSVHWQDGWHIGMLGDYPVAAQTMDIQLCFSRDGRHWLRPARQPWIPR
                     GPEDYDRKMICAPNRLADLGDSWLVLYTGMNFLHNEEGQLSNRKASICGAKLSKNRFL
                     GLKTQPQGKLLTKPFILLNEKLTLDADIRGSVRAELCDPYGKALEGFAMADCVPMTGN
                     SVQHEIAWNNGNYRQYQYESLSLRLQLDDATLYAVGL"
     gene            complement(3792..4193)
                     /gene="mscL"
                     /locus_tag="PKH07_01055"
     CDS             complement(3792..4193)
                     /gene="mscL"
                     /locus_tag="PKH07_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014705740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HNT33568.1"
                     /translation="MSIMKEFKAFAMRGNVVDMAVGIVVGGAFGKIVSSFVADVIMPP
                     IGMLLGKVNFADLAVTLKEASGDVAAVTLNYGKFVQTMVDFVIIAFAIFMVTKAMNTL
                     KKKEEQAPVAPPVPTKEEQLLTEIRDLLRQR"
     gene            4378..5904
                     /locus_tag="PKH07_01060"
     CDS             4378..5904
                     /locus_tag="PKH07_01060"
                     /inference="COORDINATES: protein motif:HMM:NF022623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HNT33569.1"
                     /translation="MKTCYPVLHSLSLGILLGGLVVVLSGCSGQGSEDTSSSHGSSGR
                     GQFALNSPLANLARLQDYQSARASSHDPGGTNTDYLVIKPWGTATFGNLKGPGCITRI
                     WMALNSDAEFYLKRLVLRIYWDDEKTPSIEVPIGDFYGAGFGQYNHYISLAMSIKYYW
                     GLNCYLPMPFEKSARFEVVNESDKQVKALFYQIDYCTFDRLPDRMLRLHAWWNQEMPC
                     FDESYDILNVRGDGQYVGCFLHVRTNERGWWGEGDDRIYIDGDTEPTLYGTGSEDYFG
                     GAWMFNKDYPDAYSGCMFLEGSDTSGRCSMFRFHLEAPVRFLKSFRFEFEHDREDDYC
                     STAFWYQKEPHEPMPPLPPTLHRLPRGAQMVLPDAHDFEALTLVEGQPEVAREVISVT
                     PGSLPMHQGQRLVLRSKSPFSLSFQLPSLQSGTYRARIYSGRGAQCGVWQCILDGQSV
                     GERFDGYSLREDLGTPNDLGTVKLDSGVHTLEVRVADKNPNSTGYDCYLDCIEFTPTE
                     "
     gene            5932..>6318
                     /locus_tag="PKH07_01065"
     CDS             5932..>6318
                     /locus_tag="PKH07_01065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33570.1"
                     /translation="MKCATKNKIRLAGLIVALALVAFGQLFFSKGDQSKGLWLFVPGL
                     VVAVVSARARVPEREYANSVKESTDLCRDTTKPGPCPERVPSPSPARRIPIWLEILIF
                     VVIIGSGFAFRYYRITEIPPCLFDDES"
BASE COUNT         1456 a         1627 c         1715 g         1520 t
ORIGIN      
        1 tgaatctcga actggcgcag tcgaacgatg tgtctgactc ttcagagtca gcctgggctc
       61 cacgacagag taacacacca gtctccgaga acgacgctgt cgaacgtgcc aaactggtca
      121 ttcacggtct tctcctcggg gtgtccgaaa gcattgagtc gctgctgacc gattcgacaa
      181 agcacaccga cagcattgaa cagcataaag tggcagtcaa gaaagccatg actgtggccg
      241 gtatcaagga gctggagcgg ttgattctgg atgaactgga gaagatgcgt tcatccaacg
      301 atcagtacag aggccaactc agtgaagcca acggtcgtct taagattcaa caggatgttc
      361 tggagaaact tcaggcggac gttcggatgg actttctgac gaaactctcc aatcggcgtg
      421 cctttgacac acggtttgtg gaagaaacag agcgcgcccg ccgatatgga catcagctgt
      481 ccctcattct gatggatgcg gacaggttca aagccatcaa cgatgtctac ggtcatgtgg
      541 cgggcgatcg catcctgcgc ggtgtggcaa aggtcattga aggggagacg cgttcctcag
      601 atttcgtggc acgctatggg ggcgaggagt ttgtgctgct cttacctgag acatccatgc
      661 agcaggcctc tgcagttgct gagaagatca gaaagaagat cgagcagacc aagttctggg
      721 tggacacgcg caatatcaag gtgaccgtta gccttggtgt tgcccagttc gatgcacaga
      781 atgacactcg ggagtccttt ctcaaacgcg cagacgctgc catgtaccgc gcaaaggaag
      841 ccggacgaaa tcgtgtggag tgctgaaatc tcacaacccc tatcgaataa tgcctcttga
      901 aactgatatc agaatccttc atcccctcat cgaacgactt gtcgaactgg ccaatctgcc
      961 ctctgaagac agaaaaaaga gactctgggc agaccatcaa gccttgttgc cgacaaggaa
     1021 gattccagtg tgcgtctact atgaaggtat cccaggctct caatgggata ccatgtttgg
     1081 aagtgaccat ctgcaaactg aggattctct cgcccgttcc attgagtttg atcttaagag
     1141 gcgtatctgg atggcggaga atgttccaga cgatcacatc gtctggccgt cagtgacgat
     1201 ccatgcaccc atgaagattg ttcagaactg gggagtctca attcagtggt cgtcgagcat
     1261 ccatcctctg ggggcgcgag gatacaaacc accctttgaa gaacagatcg atctctccca
     1321 gctcacccaa cctgtctatc ggtttgacac ggaagccact cagactttga aggacaaggc
     1381 cactgaactg acccagggaa tgttggatgt tttcgtacac tatgccagca tgggctatgc
     1441 gcccttcgat gttgccactt cgatgagagg catgacgaac ctgatgatgg acgttgttga
     1501 gtgtccagac caggtcactg gactgatggc tttcctgact gatgcggaaa ttgcgcatga
     1561 gacgtggcgg gaacagaaca actgtatcaa cgttttcccc gacaggaccc agacctatca
     1621 acaagttggg tttcgcgttc attgtgctta tcttccagca ggttttggag acagaaaggc
     1681 aagacttgag gacgagtgga cttatttgtc tcagcagaca tctgcgggac tgggccctga
     1741 gcagtatcgc gattttgttc agcctttcaa cgtggcactg gcatcgttgt tttcgcagga
     1801 gaccgtttac tatcacgggt gtgagtgcct ggatcagaaa ctcgctgttc ttgcttcgac
     1861 accaaacttg aggcggttcc atatttctcc atggagttca gttgccaagg ccagagatgt
     1921 gtttcagggc aagatggtgc tggaagtcca tgatcatcca ggacgagttt tctttggcgc
     1981 tactcgcgaa gagatcagaa cgaacctgcg ctgccttgtt cagcaagcgc aaggtcaccc
     2041 catggatctg aacatcagcg acattcactc gtttggtggc aagccggaaa tgctctccct
     2101 ctgggctgag attgcgcagg acgtggcgtc aggaaactga atcagagccc aactgcgtag
     2161 agcgttgcat catccaactg caaacgcagc gacaaggatt catactgata ctgccgatag
     2221 ttcccattgt tccaggcgat ttcgtgctga acgctgttgc ctgtcatggg aacacagtcc
     2281 gccatggcaa agccttccag agccttacca tagggatcgc acaactctgc acgaactgag
     2341 cctcggatgt ccgcatccag tgtgagcttt tcgttaagca ggataaacgg ttttgtcagc
     2401 aacttccctt gaggctgagt tttcagaccg agaaagcgat tcttggagag cttggctcca
     2461 cagatggaag cctttcggtt gctgagttgt ccctcttcgt tgtgaagaaa attcatccct
     2521 gtatagagaa caagccaaga gtctccgagg tcagccaggc ggttgggcgc gcagatcatc
     2581 ttgcggtcat agtcttcggg gcctcttgga atccaaggct gtcgagcagg cctgagccaa
     2641 tgccgtccgt ccctgctgaa gcacagttga atatccatcg tttgcgcggc gacaggatag
     2701 tcgcccagca ttccaatatg ccagccgtct tgccaatgca ctgacaggaa gtagaattgt
     2761 tggtcggtgg gatcgtcgct gtctggagtg atcagcaact cggggtcgct ccagtggatg
     2821 ccgttctcgc tgatgcggcg gtgaaccgtg cggagggaga acggcgcatt gtcgtaggca
     2881 atatgccgcg ggccgtcctt cgggttatac atcagccaga cggagtacat ctcaaaacgt
     2941 ttgagtggct cattgaagta gacaaaagtc gcgtcgttgc ttcgcagtct tcgcttgtgg
     3001 cgaagttgtt ccgcagagac tccctcgaaa ggcggcttcc cggcgatggc ctctatgtcg
     3061 cagcccatac caaagccata gggctggagt tcggacggat ggaaaatggc cgcctggttc
     3121 acatcatcaa cacgccatga aagcccgtct ttgctgtgcg cgatggcgta tcgtaaatag
     3181 ccaccgtgga gccagaagta catgcgccat tcattttcac tcagtcggaa gacttgtggc
     3241 tggccatagt tcaccttgtt gttgggatct ggaattccct cgatcttcaa gagattggtg
     3301 tctttgccct caaactgaac ctgaccgagg ttggggcgcg tccagtgcaa cagatcttcg
     3361 ctctcggcaa cacccattcc caatcgccaa gtcagtcggt gctgagaact cgagctgggt
     3421 tggcaggtgt agtagaaacg ccatcgcccg ccgtcgagtt gcacgaggct gctggcgaaa
     3481 ctggcgacga aaggaccatc cgaggcaatc ggatcgatca ctggaccgat cttctccaca
     3541 gggcctggca ctcgggtcag accttcctgt tcggcaaggt gggcatcatc aaagaagtgt
     3601 aacatgatag gcgctcctca tatcatctgt tttctgccac cgatatcatc atagcacaga
     3661 gtcatctgga caacgcaaag gcaaatgcag cagttcaagg aggtcaattc cagtcattgg
     3721 cctcgaaggc gtcaaaaagt ctgctcagcg aaattcgcta gatgagacgc tcagggatcg
     3781 aaatcccacg actatctttg tctcaggagg tcgcgaatct ctgtgagaag ctgctcctct
     3841 ttcgtcggga caggaggggc aaccggggct tgctcttcct tcttcttgag cgtgttcatt
     3901 gcctttgtga ccatgaagat ggcaaaagca atgatcacaa agtccaccat cgtttggacg
     3961 aatttgccgt agttcagtgt gaccgctgca acgtccccac tggcttcctt gagcgtgaca
     4021 gcgagatctg cgaaattgac ttttccaagc aacattccaa tgggtggcat gatgacgtcg
     4081 gcaacgaacg acgacacaat cttgccgaac gcaccaccga ccacgattcc cacagccatg
     4141 tcaaccacgt tacctcgcat ggcgaacgcc ttgaactcct tcatgatact catcgcgatt
     4201 gctcctcttt cctgctgaat tgtccgaatg tctgaacgtt cgggtacttg aaacgaggtt
     4261 aatgcgctct tgggaaggag tcaaatctga gcaaaccgct gcagaccaac atggacaatt
     4321 gacaccctca atccaaacgt gaataatccc aaaaactgtg ttgaatgggg tctgaagatg
     4381 aaaacttgtt accctgtatt gcattctttg tctctcggta ttctcctcgg aggccttgtg
     4441 gttgtcctga gtggctgttc aggacaaggg tctgaggata cttcctccag ccatggatcc
     4501 tccggccgtg ggcagtttgc tcttaacagc cctctggcta atctcgcccg tctccaagac
     4561 tatcagtctg ctcgtgccag cagccatgat ccagggggta ccaatacgga ttatctggtc
     4621 atcaaaccgt ggggtactgc cacgttcgga aatctgaagg ggccggggtg catcactcga
     4681 atctggatgg ctctcaacag cgatgccgaa ttctatctga agaggctcgt tctgcgcata
     4741 tactgggacg acgagaagac tccgagcatt gaggtgccca ttggtgattt ctacggtgcc
     4801 ggatttgggc agtacaatca ctacatttct cttgccatgt ccattaagta ctactggggt
     4861 ctgaactgtt atctgccgat gccgtttgag aagagcgccc gttttgaggt tgtcaatgaa
     4921 tcggataagc aggtcaaggc gttgttctat cagattgatt actgcacctt tgatcggctg
     4981 ccagaccgga tgctccgact tcacgcctgg tggaatcagg agatgccgtg ttttgatgag
     5041 agttacgata tcctcaatgt gcggggcgat gggcaatacg ttggctgttt tctccatgtc
     5101 cgcaccaacg aacggggctg gtggggtgag ggagacgacc gaatctacat tgacggcgac
     5161 acagaaccga cactatacgg caccggctcc gaagactatt tcggtggtgc ctggatgttc
     5221 aataaggact atcctgacgc ctattccggt tgcatgtttc ttgagggaag cgacacatca
     5281 ggacgctgca gcatgtttcg ttttcatctc gaagcgccag tgcgttttct gaagtcattc
     5341 cgattcgagt ttgaacacga ccgcgaggat gactattgca gtaccgcgtt ttggtatcag
     5401 aaggaacccc acgagccgat gcctcctctt ccgccaacgc tgcaccgatt gcccaggggt
     5461 gcacagatgg tcttaccaga cgcacacgac tttgaagcgt tgactttggt tgagggacaa
     5521 ccggaagttg cccgtgaagt catctcggtc actcctggct cacttccgat gcatcagggg
     5581 cagcgtcttg ttcttcgctc caagtcacct ttttccttgt cttttcaact tccttcgctt
     5641 cagtcaggta catacagagc gcgcatctac tcaggtcgcg gtgctcagtg cggcgtttgg
     5701 caatgtattc tggacggcca gtcagttggc gagcgctttg acggctactc actcagagaa
     5761 gacttgggaa cgcccaacga cctggggact gtcaaactgg attcaggagt tcatacgctt
     5821 gaggttcggg tcgcggacaa gaatcccaat tcgacgggct atgactgtta tcttgattgc
     5881 atcgaattca caccgacgga gtgatgtaga acgacttcag ggaatgcgct gatgaaatgt
     5941 gcgacaaaga acaagatcag acttgcggga ctcatcgtgg ctctcgctct tgtggctttc
     6001 gggcagttgt tcttctcaaa aggcgaccag agcaagggac tgtggctgtt tgtgcctggt
     6061 ttggtggtgg cggtggtgag tgcgcgtgca cgggttcctg agcgggaata cgcgaacagt
     6121 gtcaaggagt ccaccgactt gtgccgcgat actaccaaac cggggccatg tcctgaaaga
     6181 gtcccctctc cgagtcccgc ccgaaggatt ccgatctggc ttgagatcct catcttcgtg
     6241 gtcatcatcg ggtctggttt tgcgttcagg tactaccgga tcacagaaat cccaccttgt
     6301 cttttcgatg atgaatca
//
LOCUS       DAOQUC010000034         6189 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_14016_length_6189_cov_3.411966, whole genome shotgun sequence.
ACCESSION   DAOQUC010000034 DAOQUC010000000
VERSION     DAOQUC010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6189
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14016_length_6189_cov_3.411966"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1045)
                     /locus_tag="PKH07_01070"
     CDS             complement(<1..1045)
                     /locus_tag="PKH07_01070"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNT33571.1"
                     /translation="MTENALIIESIKKGQTERFGELIDRYTRMVYGIAWSYLGDADAC
                     EDVAQETFLKGFQLLGSLRDPGKFGPWIARIARNAAITFGRRRRRELATEERWQIQST
                     VADESPDAENDPSLVETISGTLRQLAPEHREALVLFYLEGKSIRESSEILGLTENAFK
                     TRLYRARSKMRERMDEHLERSLRDLAPRDDLRSRVLAAIPAAPLGWGGVGGAVSSFAP
                     GLVPILLALVSKMPFLFLLSYANRGLAHNFRDENDFRRRVLWKNFTLLLVVLIPILIL
                     ASPLCLRIGFRTYSLILAVYMIPAVIQFLLLLRVNQSRYTVVQGLGCTSMLVTFLLMG
                     GLGFPFLTFVAVML"
     gene            1242..2594
                     /locus_tag="PKH07_01075"
     CDS             1242..2594
                     /locus_tag="PKH07_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018204710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HNT33572.1"
                     /translation="MSGKKILLTEAEVPQKWYNVQADMPKPLDPPLNPATKQPIGPEA
                     LAPIFPMALIEQEVSTERWIAIPEEVMDILMIWRPTPLVRAERLEKALGTPARIYYKN
                     ESVSPPGSHKPNTAVAQAYYNKKQGLKRLTTETGAGQWGSALSFACQMLGLECTVYMV
                     KVSYQQKPYRRSMMHTYGAEVFPSPTDRTNAGRGILAKDPECLGSLGIAISEAVEEAA
                     TRADTNYSLGSVLNHVLLHQTIVGLETKKQLEKANDKADIVIGCVGGGSNFAGMAFPF
                     FPDKLAGKDIRFIGVEPCACPTLTRGVYAYDFGDTAQMTPLLKMHTLGHDFIPPGIHA
                     GGLRYHGGAPLLCALINQGFIEAQAVHQTAAFKSALQFARTEGIIPAPETSHAICVGI
                     QEALKCKETGEAKSIVIMFSGHGYFDMGSYDAYLAGKLQDYEYPAELIAESLKKLPQV
                     "
     gene            2831..4366
                     /locus_tag="PKH07_01080"
     CDS             2831..4366
                     /locus_tag="PKH07_01080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33573.1"
                     /translation="MRWTWVAVVLLGLVVMGWSAAAQSSDLEAQVKKMQEQIEKQQAQ
                     LQALQEALESESKERKSSLSEIKSLQLQTDRIKIGGDIRLRGVSFHDVWDMGTAPAGL
                     PEWNDSWDFYRFRNRLWFDVNLGEADIAEEIRGYIRLVNEYRWGDTPDSIYTTDGETN
                     TTKQIEIDNAYIELKYIANTCVSLKLGRQDLIYGEGFLILDGTPWDGSRTIGFDAIKA
                     TLDFDHTIVDVFMAKVFENDRGQGDDENLLGVYATNKELLNGHTIEAYVLHQDNNEQY
                     YTGHPTAGRGKLIPSFNVTTVGTRLSGKLTDNLKYAGEIAGQWGNNNSAYDSRFGGIS
                     VDASSDNAIGGYLNGTYCMNDLSLKPELTAGFTYLGEGWKSMYGNWPKYSELLIYTFY
                     DGFDYFKPTNTDPTVGTWTNMMFPSVAMTVKPTEKMTASLGYRYLIAVDERSNSELMF
                     DSGGNVVDGDNIGSLVQAMVKYQFTKNLSSHALIEYFSPGDYYPDYFNDAYFARWELM
                     LSF"
     gene            4588..5757
                     /locus_tag="PKH07_01085"
     CDS             4588..5757
                     /locus_tag="PKH07_01085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33574.1"
                     /translation="MGIVFLVSVAFGIKAYQALGVEQLDRASFEPAVFSLSLSCAEVL
                     WQGCAPLLRHRNAVELKVEHRISFSTATQNLFRQCGFAQPDLYYDWETDKGFLSGASS
                     PKARWNPADDHGVATVTVRGRLVLTPPPEKGWLWSKKREPLEYYASTRQSFVIPSTYE
                     QASADPENRLVIGTYPVALSAAAERYPKIHPDRYRVPDFFYKVTAETAQTPISPHFRI
                     GDFDMCYPYLDYEYPQYIAVTVPLVQKLELLLERMNQDNHSLSSFTLISGFRPPEYNR
                     GSIQKGADLKKPFSRHQYGDAVDMIIDVNPRDDVMDDLNGDGVIDIRDAEVVLKYVDE
                     LDARFLKEGSPMMGGAGVYSRHDIPERPKQSPYIHIDTRDFTNSYGLPVRWRESE"
     gene            5913..>6189
                     /locus_tag="PKH07_01090"
     CDS             5913..>6189
                     /locus_tag="PKH07_01090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33575.1"
                     /translation="MDSWNEIWSKLTDAGWQLARSEILRETGLSWLVDALHCDLKAYY
                     VVEAETLETAMEKLDAAIHTGKGMSSDCLGMCTRRIPSECPLSLDGYC"
BASE COUNT         1503 a         1549 c         1665 g         1472 t
ORIGIN      
        1 caagcatcac agcgacaaag gtcaagaaag ggaaacctaa gccccccatc agcagaaagg
       61 tgacaagcat ggacgtgcac ccaagcccct gaaccactgt gtaacggctt tgattcaccc
      121 tcaacagcag aagaaactgt atcacagcgg gaatcatgta gactgccaag atgagggaat
      181 acgttcggaa gccgatgcgc agacacaacg gacttgccag gataaggatc ggaatcaaga
      241 ccaccaggag cagagtgaag ttcttccaga gcactcttcg gcgaaaatca ttttcatctc
      301 gaaagttgtg cgccaaacct cgattcgcat agctcagaag aaacagaaaa ggcatcttgg
      361 aaaccaatgc caggagaatt ggaacaagcc ctggcgcaaa agaactcaca gcacctccca
      421 ctcctcccca tccaagcgga gccgcgggga tcgcggccag aacgcgagag cggagatcat
      481 ccctcggtgc gagatctcga agcgagcgtt ccaggtgttc atccattctt tcgcgcatct
      541 tgcttcgcgc tcgatacagc cgtgttttga aggcgttttc tgtcagacca agaatttcgg
      601 aagattccct aatgctcttt ccctccaggt agaacaggac aagtgcttcc cgatgctctg
      661 gcgccagttg cctgagagtt ccgctgatgg tttccaccag agatggatca ttttccgcgt
      721 cgggtgactc atcagcaacc gtgctctgaa tctgccagcg ctcttcggtc gccaattccc
      781 gtcgccgtct gcgaccaaag gtgattgcag cgtttcgagc gattcgagcg atccaaggac
      841 cgaattttcc cggatcccga agagaaccaa ggagttgaaa gcctttgaga aatgtttcct
      901 gtgcgacgtc ctcgcacgcg tctgcatcac ccaaatagct ccaggcgatg ccatagacca
      961 tccgtgtgta gcggtctatg agttctccga aacgttcggt ctgccccttc ttgatggatt
     1021 cgatgatgag tgcgttttcc gtcatctcgc caagatttcc tttctttgca ttgtctgcct
     1081 aagagacgcc aaacgcaaga aaaggttaca ggttccgtac cggaaatttt ggtgcaggaa
     1141 tctcttgact tttcgtgaat tctgtcacac tgtactcgga actcaaacgg agtaactttt
     1201 gttttcattg tgatcacaca acatggagag ggcttagaat tatgtccgga aaaaagattc
     1261 tgttgacaga ggccgaagta ccgcagaagt ggtacaatgt tcaagcggat atgccgaagc
     1321 cgcttgaccc accattgaat cctgcgacca agcagccgat cgggccagag gcgttggcgc
     1381 caatcttccc aatggctctc attgagcagg aagtcagcac ggagcgctgg atcgccatcc
     1441 cggaagaggt gatggatatt ctgatgatct ggcgaccgac tcccttggtt cgcgccgagc
     1501 gcttggagaa ggcgttggga acgcccgcgc gcatctacta caaaaacgag tcggtgagtc
     1561 ctccgggcag tcacaagccc aacaccgcgg tcgcccaggc gtactacaac aaaaagcagg
     1621 gactcaagcg cttgacgaca gagacgggag ccggtcagtg gggcagtgcc ttgtcctttg
     1681 cctgccagat gcttggcctc gaatgcaccg tctacatggt gaaggtcagt taccagcaga
     1741 agccctatcg ccgctcgatg atgcacacct acggcgcgga agtctttccc agcccgaccg
     1801 atcgcacgaa cgcgggtcga ggcatccttg ccaaagatcc cgagtgtctc ggcagccttg
     1861 gaatcgcgat cagtgaggca gtcgaagaag cggcgactcg tgcggatacc aactactctt
     1921 tgggcagcgt tctgaaccat gtgctcttgc atcagacgat cgtcgggctt gagaccaaga
     1981 aacagctcga gaaagcgaac gacaaggctg atatcgtgat tggttgtgtt ggtggcggaa
     2041 gcaatttcgc tggaatggct ttcccgttct tccccgacaa acttgcaggc aaagacattc
     2101 gcttcattgg agttgaaccg tgtgcgtgtc cgactctgac gcgaggggtc tatgcttacg
     2161 actttggcga tacggcccag atgactccac ttctgaagat gcacactctg ggtcatgatt
     2221 tcatcccgcc cggaatccat gcgggtggtc tgagatatca cggtggtgct ccacttctgt
     2281 gtgctttgat caaccaaggc ttcattgagg cgcaggcagt tcaccagaca gcggctttca
     2341 agtcagcgct tcagtttgcc cgaactgagg gcattattcc tgctccggaa acgtcgcacg
     2401 ccatctgcgt cggcattcag gaagcgctga agtgcaaaga gacgggtgag gcaaagtcca
     2461 ttgtcatcat gttcagtgga catggatatt tcgatatggg ttcctatgat gcctatttgg
     2521 ccggcaagtt gcaggactac gagtatccgg ccgaactcat tgccgaatcc ctgaagaaat
     2581 tgcctcaggt ttagagggcg ttattttttt cacacaatgc tcattttgtt cttgcattgt
     2641 ggcaactagg ggttgtatag ttcggcttaa ttcatcctaa ataggtgagg gggtgagttg
     2701 tacaggttcg tttggtctga acaggaagat tcctgtaggt ttcttttgct ttctttattt
     2761 ggcctgtttt aggcaggagt tttatagatt ctgtttctga acagagaaaa tgcaaggagg
     2821 agtggaaaag atgcgttgga cttgggttgc ggtagtgctt ttgggtttgg tggttatggg
     2881 ctggagcgct gcagcgcaat cttccgacct ggaagctcag gtcaagaaga tgcaggagca
     2941 gattgagaag cagcaggctc agttgcaggc gcttcaagaa gcgttggagt cagagtcaaa
     3001 agagcggaag tcttcccttt ccgagatcaa gagtctgcag ctgcaaacag accggattaa
     3061 gatcggtgga gacattcggc tgcgcggtgt ttccttccat gatgtttggg atatgggaac
     3121 agcaccagct ggactgccag aatggaatga ctcttgggat ttctatcgtt tccgtaacag
     3181 actttggttt gatgtgaatc tgggtgaagc cgacatcgct gaagaaatca gaggctacat
     3241 ccgcctggtg aatgagtacc gatggggtga cactcccgat tcgatttaca ccaccgatgg
     3301 cgagaccaac acgacaaagc agattgaaat tgacaatgcc tacattgagt tgaagtacat
     3361 tgccaatacg tgtgtgtcgt tgaaactggg acgtcaggat ttgatctacg gagaaggctt
     3421 cctgattctc gatggaacac cgtgggatgg ttccagaacg attggctttg atgcgattaa
     3481 ggctactctg gactttgatc acaccattgt ggatgtgttc atggcgaaag ttttcgagaa
     3541 cgatcgcggt cagggtgacg acgaaaacct gctaggcgtc tatgcgacca acaaggaact
     3601 gctcaatggg cacacaattg aagcctatgt tcttcatcag gacaacaacg agcagtacta
     3661 cacaggacat ccgactgctg gcaggggcaa gttgattccc tctttcaacg taaccaccgt
     3721 cggcacacgt ctgtccggca aactgacaga taaccttaag tacgcgggcg aaatcgctgg
     3781 ccagtggggc aacaacaaca gcgcttatga ttccagattc ggaggaatct ccgttgatgc
     3841 atccagtgac aatgcgattg gcggctacct gaatggtact tactgcatga atgacctttc
     3901 cctgaagccc gagttgacag ccggatttac ctatctgggt gaaggctgga agtccatgta
     3961 cggcaactgg ccgaaataca gcgagctgtt gatctacacg ttctacgatg gattcgatta
     4021 tttcaaaccc accaatacag atccaactgt aggaacctgg acgaacatga tgttcccctc
     4081 ggtcgcaatg acagtgaagc ccacagagaa aatgaccgcc tcgttgggat atcgctacct
     4141 gattgctgtt gatgagagat ccaacagcga gctcatgttt gacagcggtg gcaacgtcgt
     4201 tgacggagac aacattggaa gtcttgtgca ggcaatggtg aaataccagt tcacaaagaa
     4261 cctttccagc catgccttga ttgagtactt cagccctgga gactactatc cggactactt
     4321 caacgatgca tactttgcac gttgggaact gatgctgtcg ttctaagatt cgcaactcag
     4381 gtcaacataa gaccgaatct tacggaggag atccttgatg ggtctcctcc gttgtttttg
     4441 cgtttcggtt ctcgggctct gttgttgaac tgtatttgag aagctttact ttctgtttcg
     4501 atccaattat tcttcttcct gtatatgaaa gagagaaaga agagagtcgc aaagaaaccg
     4561 aagcgtttcc tgccgtgggt gttgtgtttg ggaatcgtgt tccttgtttc tgtcgcattt
     4621 ggcatcaagg cctatcaggc tcttggtgtg gaacaactgg acagggcctc atttgaacca
     4681 gcggtcttct cgctgtcctt gtcgtgcgct gaggtgcttt ggcagggatg cgctccactg
     4741 ctgcggcatc gcaatgcagt ggagttgaaa gtagaacaca gaatctcctt ctcgacggca
     4801 acacagaacc tctttcggca atgtggtttt gcccagcctg atctttacta tgactgggaa
     4861 acggacaaag ggtttctgtc cggagcatca tctccgaaag cgcgatggaa tcctgctgac
     4921 gaccacggtg tcgccacagt tacggtgcgt ggcaggcttg tgctcacccc gccacctgag
     4981 aagggatggc tctggtcgaa gaaacgggag cctctggagt actatgcttc aacacggcag
     5041 tcctttgtga tcccgtctac ctatgaacag gccagcgctg atcctgagaa tcgtctggtc
     5101 attggcacgt acccagtggc tttatccgct gcagcggagc gctatccaaa gattcatccc
     5161 gaccgatatc gggtgcccga cttcttctac aaggtcacag cagagaccgc ccaaactccg
     5221 atctcaccgc atttcagaat cggggacttc gacatgtgct acccctacct cgactatgag
     5281 tatccccaat atattgcggt cactgttccc ttggttcaga agcttgaatt gcttctggag
     5341 cggatgaacc aagacaacca cagcctctcc tctttcactc tcatcagcgg attccgaccg
     5401 cctgagtaca accgcggctc cattcagaaa ggagccgatc tcaagaaacc tttcagtcgt
     5461 catcaatacg gtgatgcggt ggacatgatc attgacgtca accccagaga tgatgtcatg
     5521 gacgatctca atggagatgg tgtaattgac attcgggatg cagaagttgt gctcaagtat
     5581 gttgatgagc tggatgccag attcctgaag gaaggcagtc ccatgatggg cggtgcggga
     5641 gtctattccc gacacgatat tcccgagcgc cccaaacaga gtccgtacat ccatattgat
     5701 acgagagatt tcacgaacag ttacggattg cccgtccgct ggagagagtc tgaataggat
     5761 ggacgtcgag aaccttgaga aaccactcat cccgagcagg cctgcgcctg agggcacgtg
     5821 ggctatgctc actcttacaa acgcttgctc attggaggga acagacttct ttagagtctg
     5881 gtttcccagg agcatgaagg aatcacccca ctttggactc atggaatgaa atatggagca
     5941 agctcacgga cgcgggctgg cagcttgcga gaagtgagat tctgcgcgaa accggactgt
     6001 cttggctcgt ggacgctctg cactgcgatc tgaaggccta ctacgtcgtt gaggcagaaa
     6061 cgcttgagac agccatggag aagcttgacg cagcgattca tacgggaaag gggatgtcat
     6121 cagactgcct tgggatgtgc acgcgacgca tcccaagtga gtgtcccctg tctctggacg
     6181 gctattgcg
//
LOCUS       DAOQUC010000035         6140 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_14172_length_6140_cov_3.495152, whole genome shotgun sequence.
ACCESSION   DAOQUC010000035 DAOQUC010000000
VERSION     DAOQUC010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6140
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14172_length_6140_cov_3.495152"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1134)
                     /locus_tag="PKH07_01095"
     CDS             complement(<1..1134)
                     /locus_tag="PKH07_01095"
                     /inference="COORDINATES: protein motif:HMM:NF024120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HNT33576.1"
                     /translation="MMEQFTNRRGFLTQSALAAGTIGLGACSTTTKMDAQPATVTSAS
                     VRDFGAVGDGKHDDTEALREAFKSIPSGTAVHLPRGDYVLSGVVDIPPTCALMGEPFG
                     ARLIVRHYDAPAIRVAGETRLSDVAILYPDNTDLANPRECPESVSLVGNGAGYVENIT
                     FINAFIGVGTPAEGANCGQSVIRKLNGFVHDTMVRIDGSLDIVRIENVHCFVAMGEEE
                     PNRSYYRQHRKCFHIKGSDGTLISKSFMIFGKVFLLKEAGHHGSGLSTYLSQCWLEGM
                     SDRGILLNDGSRMSLVGVELTCAYAKAVIEVNNGGFLRASSCYIRDSMHSKGIVVNGE
                     SAVIVGDCEFFGSPGFTGIEINSTGRSIISNNFLHHCEVGIHCT"
     gene            complement(1200..2066)
                     /locus_tag="PKH07_01100"
     CDS             complement(1200..2066)
                     /locus_tag="PKH07_01100"
                     /inference="COORDINATES: protein motif:HMM:NF012925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HNT33577.1"
                     /translation="MNPFRFLFSLTVLCLVVPVPEVRAESSQPSTLDYSRLKLVWSDE
                     FDGTSLDSTKWDVPNKEPRHGGSVWMADQVSVSDGILRLGISLITDPTNSYKCGAVRT
                     RARYTTDLFSQTYGYFEARCQLPKNVGADYWAAFWMMAGRVTDDEPSTRNGSEIDIME
                     SFTFDREAAHQLTLHWNGYGKKHNSWSLPCGNHPQLLDGEYHTFGLYWDKKKYISFVD
                     GVEVGRTDFLNKGSDKDGKVPSKGPCRKPGYIKLSCEASTWAGGAGWEDPLPTSDEFL
                     VDYVRVYRGRLR"
     gene            complement(2278..2748)
                     /locus_tag="PKH07_01105"
     CDS             complement(2278..2748)
                     /locus_tag="PKH07_01105"
                     /inference="COORDINATES: protein motif:HMM:NF021487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HNT33578.1"
                     /translation="MSVNPDYKELLSVFNEEGVEYLVVGAHAVIFYTVPRYTKDIDLW
                     VNPTAENAARVYRALAKFGSPLEGTDVASFQDANCLFQIGVEPNRADVLMGVAGLVFE
                     EAWSRRARGSYGGVPINILSLSDLIRSKEAVGRAQDMVDVQRLRKAKPLTGGES"
     gene            3095..4366
                     /locus_tag="PKH07_01110"
     CDS             3095..4366
                     /locus_tag="PKH07_01110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33579.1"
                     /translation="MRICLSVVTLSLLLQCVAFSDSNSSWNDQQWRAFCKENRLRPEG
                     WQSAKPAKGFPIGIWFDGRVHGINVPEGSVNVPAGEAAARTYYREHFTRIKNRGIPII
                     VIPNTPPEYRPWLLDEAQKVGVRIVLEIVEFVDLMHDSSCAADDWDKMLSVVRDVHKQ
                     IGGYRSLLYYQLIDEPVAALSAKLQALTRILGGVDPRRAAFSCLCGIGDKEQLIKEWN
                     PSMVVYDCYPLRKDSPVGDFGGFCPSVDTAFRFADGRPLWMVVQTCAHTEGGLRIPTP
                     AEIRAMTYLSLAHGAKGLFFFLYNSNTQTERLIGLDVLPEHFAEVADLTAKMKSLSRQ
                     LPNLTRIGLIQSKPTTLDVQEFRDVSGRGFLFVVNTDVQQVFNGNMTIEVTSSEWGRL
                     EDCLTRERYEMAEKGFRLTLLPGEGKLLRCR"
     gene            4395..5714
                     /gene="xseA"
                     /locus_tag="PKH07_01115"
     CDS             4395..5714
                     /gene="xseA"
                     /locus_tag="PKH07_01115"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HNT33580.1"
                     /translation="MEDTLFTAIEQKADDPQVYSVSALTEEIKLLLETTFGEVWVEGE
                     VSNFRISPSGHAYFRLKDEKAILECVIWRTYLAGRKDLLQDGLMVRILGELSVYPPRG
                     GYQMVARLVRPAGKGALQERFERLKATLMAEGLFDESRKKPIPPLPRCIAVVTSPGGA
                     AIQDFLKILDSRFPHFRVVIAPARVQGIEAPGEIAQALADLNRWTVPDVIVVTRGGGS
                     LEDLWAFNEEVVARAIFASEIPVISAVGHEIDFTISDFVADLRLPTPTAAAEYLARGG
                     SDLLRELQLLSDQCIQLTLSRLESLREEIAGWQESLLRCSPVASVNVLRQRLDDLVSH
                     LVTQGMRRLETRRVRLQHEAERLMHTSKTALDGRRHAFLLLQEGIKAMDPAAVLRRGY
                     SITCVEETGEVIVCAAQVSEGTLLSTRLREGSLHSRVVPREKIQEES"
     gene            5714..5971
                     /gene="xseB"
                     /locus_tag="PKH07_01120"
     CDS             5714..5971
                     /gene="xseB"
                     /locus_tag="PKH07_01120"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HNT33581.1"
                     /translation="MARNKKTEEGTPQTFEERFQRLKDIVEQLSEGDLPLNELESVFK
                     EGISLCQLCAEDLDRVEQSVQVLIKDSEERFRTEPFEREQE"
BASE COUNT         1431 a         1641 c         1638 g         1430 t
ORIGIN      
        1 ggtgcagtga atgccgacct cgcagtgatg aaggaaattg ttcgaaatga ttgagcgtcc
       61 tgttgaattg atctcaatgc cggtaaagcc tggagaacca aagaactcac aatcaccgac
      121 gatgaccgcc gactcaccgt tgaccacaat gcccttggaa tgcattgagt cacgtatgta
      181 gcaggaactg gcgcgcagaa agccaccgtt gttgacttcg atcaccgcct ttgcataagc
      241 gcaggtcagc tccacgccaa ccagtgacat tcgagaacca tcgttgagaa gaatgcctct
      301 gtccgacata ccctcaagcc agcattggga aagataggtc gagagcccag atccatgatg
      361 acctgcttct ttcaggagaa agaccttccc aaagatcatg aaactcttgg aaatgagcgt
      421 gccatcagag cctttgatat ggaagcactt tcgatgctgc cgatagtagg agcgattcgg
      481 ttcttcctca cccatggcga cgaagcagtg aacgttttcg attctcacaa tgtcgagact
      541 gccgtcaatt cgcaccatcg tgtcatggac gaagccgttg agttttcgaa tcacactttg
      601 accgcagttg gcgccttccg ccggagtgcc caccccgatg aaggcattga tgaaggttat
      661 gttctcgaca tatcccgcac cgttgccgac aagagagacc gattcgggac actctcttgg
      721 atttgccagg tcggtattgt caggatagag aatcgcgaca tcggaaaggc gggtctcgcc
      781 tgccacacga atcgccggtg catcgtaatg tctgacaatc agtcgtgctc cgaatggctc
      841 acccatgaga gcgcaagtgg gaggaatatc cacgacaccg ctcagaacat agtcgccccg
      901 tgggagatga acggctgttc ctgacggaat gcttttgaag gcttctctca acgcctcggt
      961 gtcatcgtgc ttcccatctc cgactgctcc aaaatcacgc acacttgctg aggtgacagt
     1021 ggctggttga gcatccatct tggtcgtggt agaacaggca ccgagcccaa tcgttcccgc
     1081 cgcaagagcg ctctgtgtca agaaacctcg tctgtttgtg aactgctcca tcattcctta
     1141 tccttacaag agattcgatc gtcttcgacc tcgcaacccc acggaattcc accgccgcct
     1201 caacgcaatc gtcccctgta gacgcgcaca tagtctacca ggaactcgtc gctggtcggc
     1261 agcggatctt cccagcctgc tccgccagcc caagtggacg cttcacagga cagcttgata
     1321 tatccaggct tgcggcaggg ccctttggat ggaaccttgc cgtccttgtc agagccctta
     1381 ttcagaaagt cagtacgtcc cacttcaaca ccatccacaa acgagatgta cttcttcttg
     1441 tcccagtaga gaccaaaagt atgatactca ccgtccaaca gttgcggatg attcccgcac
     1501 ggcagagacc atgaattgtg cttctttcca tagccattcc aatgcagcgt caactggtgc
     1561 gccgcttctc ggtcaaaggt gaagctctcc atgatgtcta tttcgctgcc gtttctggtg
     1621 gaaggttcgt cgtccgtaac tcttcccgcc atcatccaga acgccgccca gtagtcagcc
     1681 ccaacgttct tcggcagttg gcaacgagcc tcaaagtatc catacgtctg agagaaaaga
     1741 tccgtggtgt atcttgccct ggtgcgaaca gcaccacact tgtagctgtt cgtcggatcg
     1801 gtgatcaatg agattcccag tcgcaaaatc ccgtcggaaa cactcacctg atccgccatc
     1861 cagacactgc ccccgtggcg aggttccttg ttggggacat cccatttggt cgaatccaga
     1921 cttgttccgt cgaattcatc agaccagacc agtttgagtc ggctgtagtc cagggtgctc
     1981 ggctgcgaag attcggctcg aacttcagga acgggcacaa caagacagag cacggtcagt
     2041 gaaaacagaa aacgaaatgg attcatggaa taacctcctg aaagttgtct gtcaagcgtg
     2101 cagaatgcgt caagcgaagg aggctttgct gcaaagcgga cacaccttct gatactcaaa
     2161 tgcaactggt gcgtccgcta cgctcgacgc acccgacaca ttatacaacc acctttgtgg
     2221 caacgcatcc cttctttgaa gagagcagct ctggccacag accccaaagg cgagatgtca
     2281 ggactctcct cctgtcagag gctttgcctt ccgaagtcgc tgcacatcta ccatgtcctg
     2341 agctcgtcct acagcctctt tggatcggat caggtcggat aggctcagaa tatttatggg
     2401 cacacctccg tagcttcccc tcgctctgcg gctccatgct tcctcgaata ccaatccggc
     2461 aacccccatc aagacgtcgg cgcggttcgg ctccacacct atttgaaaca agcaattggc
     2521 atcctggaaa gatgcaacgt ctgtaccctc aagtggagag ccaaacttgg caagcgcacg
     2581 gtagactctt gccgcattct cagccgtcgg attgacccag aggtcaatat ctttcgtgta
     2641 tcggggaact gtatagaaga tcaccgcgtg cgcgccgaca accagatatt caacaccctc
     2701 ttcgttgaag actgatagca gctctttgta gtcggggttg acagacatcg ttacctcgaa
     2761 ttgcctcgac ttggtcgatc atttcccacg ctgctgcaaa acgaacctca tggccaagtt
     2821 gacgccaata gttcgtgtcc cactcttctg cttcctcgaa agacctggcg acgttcacca
     2881 ccatgcgttt gcgccgttct gctgctctct cttccaacgt ggacatctta tcttcctcca
     2941 tcttccatga caagaatacc acgatccacc gaaaaaagga aaagtctgct tggaatcagc
     3001 tcgattcggg ttgacagttt cccttgaagc caactaacgt gatgacggtt agaatctgtg
     3061 ccgtccacat gagataccca gaggaggatg aaatatgcgt atctgccttt ctgttgttac
     3121 tctctcgctt ttgctgcagt gtgtggcctt ctccgacagc aactcgtcct ggaacgacca
     3181 gcagtggagg gctttctgta aagagaaccg actgcggcct gaaggttggc aaagtgcgaa
     3241 gcctgccaaa ggctttccaa taggaatctg gtttgatggt cgtgtccatg ggatcaatgt
     3301 tccagagggc agcgtcaatg ttccagctgg tgaggctgct gctcggactt actaccgaga
     3361 gcacttcacc cgtatcaaga atcgcggaat tccgatcatt gttatcccca acacaccacc
     3421 cgaatatcgt ccgtggcttt tggatgaagc ccagaaggtt ggcgtcagga ttgtgttgga
     3481 gattgtggag tttgttgacc tgatgcacga cagctcctgc gctgccgatg actgggacaa
     3541 aatgctctcc gtggtgcgcg atgtccacaa acaaataggc ggatatcgct cccttctcta
     3601 ctaccagctc attgatgagc ctgtggcggc gttgtcggcg aaacttcagg ctttaacgcg
     3661 cattctgggc ggagtggatc cacgccgcgc ggctttctcc tgcctttgcg gtatcggaga
     3721 caaggaacaa ctgatcaaag agtggaaccc ttccatggtt gtctacgact gttacccact
     3781 cagaaaagat agccctgtgg gggatttcgg tggtttctgt ccgagtgtgg ataccgcgtt
     3841 ccgttttgct gacggtcggc ctctgtggat ggtcgttcag acatgcgccc acacggaagg
     3901 cggccttcgc atccctacac ctgcagaaat tcgtgccatg acttatctct cgcttgctca
     3961 tggcgccaag ggactgttct tctttctcta caactcaaac acacagacgg aacgactgat
     4021 cggattggat gtgctgccgg agcattttgc agaagttgcg gatctgactg caaagatgaa
     4081 aagtctttcc aggcaattgc ccaacctgac acgaatcggt ctgattcaga gcaagccaac
     4141 gacactggat gtgcaggagt ttcgagacgt ttcagggaga ggattcctct ttgtcgtcaa
     4201 cacggacgtg cagcaggtgt tcaacggcaa tatgaccatt gaagtgacct caagtgagtg
     4261 gggacggctt gaagactgcc tgacccgaga gcgctatgag atggctgaaa aaggtttccg
     4321 cttgaccctg ttgcctggtg aagggaaatt actccgatgt cgctagtcaa ggattgggta
     4381 ttgggacgcg cgtgatggaa gatactctct ttacagccat agagcagaaa gcggacgacc
     4441 ctcaggtcta ttcagtctct gcgctcactg aggagatcaa actccttttg gagacaacct
     4501 tcggggaggt ctgggtggaa ggggaagtgt ccaacttccg aatctctcct tcaggacatg
     4561 cctattttcg actcaaagac gagaaagcca tcctggaatg tgtgatatgg cgaacctatc
     4621 ttgcgggacg aaaagatctt ctgcaggacg gcttgatggt tcgaattctg ggtgaactga
     4681 gcgtctatcc ccctcgcggg ggctatcaga tggtggcgcg actggttcgt cccgccggaa
     4741 agggtgcgct tcaggagcgt ttcgagcgac tgaaggcaac actgatggcg gaaggtctgt
     4801 ttgacgaatc gcgcaagaaa ccgattccac cactacccag atgtattgcc gtggtgactt
     4861 ctccaggagg agcggccatc caggacttct tgaagatcct cgacagtcgc ttcccgcatt
     4921 tccgtgtcgt gatcgctcca gctcgcgttc aggggatcga ggctccggga gagatagcac
     4981 aagcccttgc tgatctgaac cgttggacag ttcccgatgt catcgtcgtg acacgtggcg
     5041 gcggctcatt ggaagacttg tgggcattca acgaagaagt tgtcgcccga gccatttttg
     5101 cgtccgagat tccggtgatc tccgcagtgg gtcatgagat agatttcacc atttcggatt
     5161 ttgtggctga cctcaggctc cccactccaa cggcagcggc ggagtatctc gctcgtggtg
     5221 gcagcgatct gctgagggaa ctccagttgt taagtgatca gtgcatacaa ctgacactga
     5281 gccgactgga gtcacttcgc gaggaaattg cggggtggca ggagagcctt ctgcgatgca
     5341 gtcccgtcgc atcggtgaac gttttgcgac agaggctgga tgatttggtc agtcacctgg
     5401 tcacacaggg gatgcgtcgg cttgaaacga gacgcgttcg acttcagcac gaagcggagc
     5461 gactgatgca cacgtcaaag acagcccttg acggaaggcg tcatgcgttc ctgctgcttc
     5521 aagagggcat caaggcaatg gatccggctg cagtgcttcg gcggggatat tcgatcacat
     5581 gtgtggaaga gacgggagaa gtgatcgttt gcgccgctca ggtttctgag ggcactttgc
     5641 tgagcacacg cctgagggaa ggctctctgc acagccgagt cgttccacgg gaaaagattc
     5701 aggaggagtc gtaatggcga ggaacaagaa aaccgaagaa ggcacaccgc agacctttga
     5761 ggaacggttt caaagactga aggacattgt cgagcagctc agtgaaggag accttccctt
     5821 gaatgaactg gagtcggttt tcaaggaagg catttcgctc tgtcagctgt gcgctgagga
     5881 tttggatcgc gttgagcaaa gtgttcaggt cttgataaag gactccgagg agcggtttag
     5941 aactgaaccc tttgaacgag aacaggaatg atgtgttgcg tcagaaattg aggagactgg
     6001 attcccatct acagcagact ctatgacatg aacgcgcgag agacattcat caaatatgtc
     6061 agggaggggt cgcccgaacc ctttgtgtcc cttcagatcg gctgtggcgc tggctttgat
     6121 gtgaaactcg ccggcaagga
//
LOCUS       DAOQUC010000036         6110 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_14259_length_6110_cov_3.087036, whole genome shotgun sequence.
ACCESSION   DAOQUC010000036 DAOQUC010000000
VERSION     DAOQUC010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6110
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14259_length_6110_cov_3.087036"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            206..281
                     /locus_tag="PKH07_01125"
     tRNA            206..281
                     /locus_tag="PKH07_01125"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:239..241,aa:Phe,seq:gaa)
     gene            complement(305..2197)
                     /locus_tag="PKH07_01130"
     CDS             complement(305..2197)
                     /locus_tag="PKH07_01130"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HNT33582.1"
                     /translation="MNRFASVALALCLLAIGISSVAFANEFRGLWVDTWHNGIFDAGQ
                     VTTMINNADAYNFNAVLPEVRLRGDAFYFPTYPNIEPRNTRIGSTFDPLQDTITKAHA
                     KGIEVHAWITTFPCLSTTANSHPNHVSNKHPEWLMQNYSGSTDFGEGKYLDPGVPDAL
                     IWIYNVCMDLVTNYDIDGLHLDYIRYPGTDSGYNPIAIARFNKEFSRTGKPGTSDATF
                     ATWRRRQVTDFVKFIYLNAIAVKPNMKLTVSTFASRSDAYNARFQDWAAWMSQGIIDA
                     NVPMNYTTGSTFRSRVNDAVANKYSRHLYMGVGADDSDLVNDMLYARNAGCEGLCLYS
                     YYVGTTYNVFPAIKSSVFPTKVSQPVMTWKTSPTRGYIKGTVTSQGNIVYNANVSVVG
                     QALTSKSDGNGFYGFLNMIPGSYTVRCEAESCEPETAYVTVSAGAVKTVDFNLRLGNT
                     PTPTYTPTPWPTNVPYTNVVDDIDLGFTSVGSWTHKTDSAGASYGPSYKVTYTGTQGD
                     RAEWRPNLLYSGRYRVSIWYTSGSNRPSSVPYTVYHQSGNQTFYVNQQVNGSRWNYLG
                     EFSFSSGSSGYVSLLEQVPDGAAVIADAVLFEYVPPPSPTPTATATPRPWPTPAGGNY
                     FQVLGK"
     gene            complement(2393..3214)
                     /locus_tag="PKH07_01135"
     CDS             complement(2393..3214)
                     /locus_tag="PKH07_01135"
                     /inference="COORDINATES: protein motif:HMM:NF021507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HNT33583.1"
                     /translation="MSAMRFLYPIVILSLFLGGVVRGEDTVEPIAPGIALVHRVTTAP
                     LNAFFLKVQLDQPGVRVQPALAQDYYAARETVLSFAKRYGAVAAINGDYFGSGHGPEG
                     LNYVLGVPVYEGFGRSAVNFTSALQPTIGLDTNLYSSYQAGELELFNCLGGGPIFMTN
                     GIVKWEYTATTINGESFSAGSAWSGLQPWTAVGISSDRKLLVLAVVDGRQTLSRGVTP
                     WEMGALLVEMGASEAIKLDGGGSSEIVVNGAIRNSPSDGYERPVANALLVFSDAP"
     gene            complement(3218..4303)
                     /locus_tag="PKH07_01140"
     CDS             complement(3218..4303)
                     /locus_tag="PKH07_01140"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNT33584.1"
                     /translation="MSIGTNRLVLLMLSAVLTCSAAAIPLDWNCSGLIDSRDLFIYAQ
                     RWHSQDIPWQETPYGAGDLLTVVQSWQKQISSSAEHIPQTVEIPAGTFLMGNLRKGTD
                     RGYSTYPWSRSREFPMHWVHLDRYFIGAYEVTLEEYAEFLNDGNDAHWYSDMAHNAYC
                     GISRTGVPGSYFYQVLTGREKYPIVYVRWYDAVAYCNWLREKTQQPFRLPTEAEWEKA
                     ARWDPINETAYIYPWGDTYDSGKMNCWQASLTHVPKPVGSYPSGARYLPMGVLYDMAG
                     NVWEYASDFFQADYYKQGPGGDDPGSVVWDNPKGPSAGSARVIRGGSWANMAYSSNDN
                     DGRCAARHLTYPSHRHTTVGFRVAKGP"
     gene            complement(4306..4995)
                     /locus_tag="PKH07_01145"
     CDS             complement(4306..4995)
                     /locus_tag="PKH07_01145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33585.1"
                     /translation="MFKRLSCVFILSVILCLVANQFSVADYSCETCLTWKTCTLYEIP
                     EQRVLTDPRNGADAGCCQGGDSTPASGLNDNNNSGMICCWGMAPGPAAVNTVWFNKSA
                     VPSKVKMWFQGNPGNCSDPRDFAVSLHMPGYPDNEGWVQIINVIDKPNNDNDMEWSKD
                     SHFVGMLADGVRVHVTRSNEGVPGTGTGSWFSIAEMVVEGDLPTPTPTITPIPTATVP
                     SGVSQQLYEAY"
     gene            complement(5543..5674)
                     /locus_tag="PKH07_01150"
                     /pseudo
     CDS             complement(5543..5674)
                     /locus_tag="PKH07_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003112842.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
     gene            complement(5778..>6110)
                     /locus_tag="PKH07_01155"
     CDS             complement(5778..>6110)
                     /locus_tag="PKH07_01155"
                     /inference="COORDINATES: protein motif:HMM:NF039905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sphingosine kinase"
                     /protein_id="HNT33586.1"
                     /translation="FSLVAIGNGAFSGGGFRLTPNALPDDGLLDVCLVSQCSRCRLLR
                     CLPSSLNGTHGRHSIVTFHRVRHLTIASQEPIQAQVDGELLEPRHLWHIHVEPSRLAF
                     LKPASIVS"
BASE COUNT         1463 a         1558 c         1631 g         1458 t
ORIGIN      
        1 catttctgga agctctcttc tcctgagcag gccagcgcct gcggatgcct gctcggcaga
       61 tgacacgctc gatccttgct gagtgtcatt gggaaagcgg acgggggagg ctggtttcca
      121 gtctgtggaa ataatgagtt ttgaatgaac ggggggttga caggctaagt ctggctgtgc
      181 gatattacgc ctcgtttgtt cggttggccg gatagctcag ttggtagagc gaaggactga
      241 aaatccttgc gtccccagtt caagtctggg tccggccacc attccctctc aaccgcatca
      301 tttttcactt tcccaatact tggaaatagt tgccacctgc tggcgttggc catgggcgag
      361 gcgtggctgt tgcagtgggt gtcggactcg gtggcggaac atattcgaac agcacagcat
      421 ctgcgatgac cgcggcacca tctgggactt gttccagaag agaaacgtat cccgaagagc
      481 ctgaactgaa ggagaactcg ccaaggtagt tccatcggct tccgttcacc tgttggttga
      541 cgtagaaagt ctggttgcct gattggtgat agacagtgta gggaacactt gagggtctgt
      601 tgcttcctga tgtataccag atcgagacgc gataccttcc agagtacagc agatttggtc
      661 tccattcagc acggtctcct tgggtacccg tataggtcac tttatatgag ggtccataag
      721 aagcaccggc cgaatccgtc ttgtgtgtcc aggagccgac actggtgaaa ccaaggtcaa
      781 tatcatccac aacgttcgta taaggaacgt ttgtcggcca cggagttggg gtgtacgttg
      841 gtgttggtgt gtttccaagt ctgaggttga agtcaactgt tttcactgct cctgctgaga
      901 ctgtcacata cgccgtctcg ggttcgcaac tttccgcctc gcagcgtact gtgtatgaac
      961 cagggatcat gttgagaaag ccgtagaagc catttccatc actttttgaa gtcagtgcct
     1021 gtccgactac ggacacattt gcattgtaga cgatgttgcc ttgagaggtg acagtgccct
     1081 tgatataccc tctggtggga gatgtcttcc acgtcatcac tggctgagag accttggtcg
     1141 gaaagacgct cgacttgatt gcagggaata cgttgtaggt tgttccaaca tagtagctgt
     1201 agaggcaaag gccttcgcaa ccagcgttgc gggcatagag catatcattg acgagatcac
     1261 tgtcgtccgc ccccacgccc atgtagagat gtctgctgta cttgttggca acggcatcat
     1321 tgacgcgact tcggaaggtg cttcccgttg tgtagttcat ggggacgttg gcatcaatga
     1381 ttccctggct catccacgca gcccagtcct ggaatcgagc gttgtaggca tcggaacgac
     1441 ttgcgaaagt cgagacggtg agtttcatgt tgggttttac cgcaatcgca ttcagataga
     1501 tgaactttac gaaatcggtt acttgccgac gtctccatgt ggcaaaagtt gcatcacttg
     1561 ttccaggctt tcccgtcctg ctgaattcct tgttgaagcg tgctatcgcg atagggttgt
     1621 agccggagtc agtacctgga tatcgaatat agtccaagtg cagaccatca atgtcgtagt
     1681 tggtgaccaa gtccatgcag acgttataga tccaaatgag tgcgtcagga acacctgggt
     1741 caagatactt gccttctcca aagtcagtcg agcctgagta gttttgcatc agccactctg
     1801 gatgtttgtt ggagacatga tttggatgcg agttcgccgt tgtgctcaaa caggggaatg
     1861 tagtgatcca ggcatgaact tctatcccct tcgcgtgtgc ctttgtaatc gtgtcctgca
     1921 gcgggtcgaa agtggatccg atgcgggtgt tgcgaggttc gatattcggg tatgtgggga
     1981 aatagaaggc atcgccgcgt agacgaactt caggcagcac tgcattgaaa ttgtatgcgt
     2041 ccgcgttgtt aatcattgtg gtgacttggc ctgcatcgaa aatgccgtta tgccatgtgt
     2101 ctacccacaa tcctcgaaat tcattggcaa aagcgactga agatatgccg atggcaagaa
     2161 gacagagtgc aagtgcaaca gaagcaaagc gattcatcct aaccttcctc aatacaaaat
     2221 ggcatccaaa tccatggtcc gactggcacg tcacaggaaa ccgaacgagc caacggactc
     2281 ccaaagagga cactttttgg gaacatagca tgctgttgat tgtgcgacaa cactttttgg
     2341 gtgagtttga acagaccgat gcccttcggc aaagtgtctg gtgaggtggg tgctatggag
     2401 cgtccgaaaa gaccagcagc gcgttggcta caggtcgttc atagccgtcc gatggggaat
     2461 tgcggatcgc tccattgacg acgatttcgg aggaaccgcc accgtcaagt ttaatggctt
     2521 ccgaggcgcc catttcaacc agcagagccc ccatctccca aggggtgaca ccacgactga
     2581 gtgtctgtct tccatctaca accgccaaga ccaacaactt ccgatccgaa gagataccga
     2641 cagctgtcca aggttgaagg ccactccatg cagagccagc agaaaaactt tcgccgttga
     2701 ttgtcgttgc ggtgtattcc catttgacaa tgccattcgt catgaaaatg ggacctccac
     2761 cgaggcagtt gaacaattcc aattcccctg cttggtaact ggaatacaga ttcgtgtcga
     2821 gtccgatagt gggctgcagt gccgatgtga agttgacagc gcttcttccg aaaccttcgt
     2881 atacgggcac tcccagcaca tagttcagcc cttcgggacc gtgaccgctt ccgaagtagt
     2941 ctccattgat tgccgcgacc gcgccgtaac gtttggcaaa agatagcacc gtctcacgag
     3001 ccgcatagta gtcctgtgcc agagcaggct gaacgcgtac accgggctga tcgagttgga
     3061 ccttgaggaa gaaggcattt aagggcgcag tcgtcacccg atgcacaagc gcaatcccag
     3121 gagctatcgg ttcaacagta tcttctccac ggacgacacc gccaaggaag agactgagta
     3181 tgacaatcgg atagagaaac ctcattgcac tcattgatta gggtcctttt gccactcgaa
     3241 agccgaccgt tgtatggcgg tggctggggt aggtcagatg tcgagcggcg cacctgccgt
     3301 cgttgtcatt cgaagaatat gccatgtttg cccaactgcc gccgcggatt actcgggcag
     3361 agccagctga aggccccttg gggttgtccc ataccaccga gccgggatcg tcacctccag
     3421 gtccctgttt gtagtagtca gcctgaaaga agtcactcgc gtattcccag acatttccag
     3481 ccatgtcgta gagaacaccc attggaaggt atcgggcacc agaagggtag gaaccaactg
     3541 gtttgggaac atgcgtcaga gatgcctgcc agcagttcat cttgcctgaa tcgtacgtgt
     3601 caccccaagg atagatataa gcagtttcgt tgatcggatc ccagcgtgct gccttctccc
     3661 attccgcttc tgtcgggagt cgaaaaggct gttgggtctt ctctcgaagc cagttgcagt
     3721 aggcgacggc atcataccat cgaacgtaga caatcggata cttctcacga cctgtcagga
     3781 cttgatagaa atagctccct ggtacaccag ttctggagat gccgcagtaa gcgttgtgtg
     3841 ccatgtcgga gtaccagtga gcatcgtttc catcgttgag gaactcagcg tactcttcaa
     3901 gggtgacctc gtaagcgcca atgaaatacc tgtccagatg tacccagtgc attgggaact
     3961 cccttgatct gctccatgga taggttgagt accctcgatc ggttcctttt cgaagattcc
     4021 ccatgagaaa agtgcctgca gggatttcga ccgtttgggg aatgtgttcc gccgaggaac
     4081 taatctgttt ctgccatgac tgaaccaccg tgaggagatc accagcaccg tatggagttt
     4141 cctgccaagg aatgtcttga gagtgccaac gctgggcata gatgaacaaa tcacgactgt
     4201 ctatcagacc tgaacaattc cagtcgagcg gtatcgcggc ggcagagcat gtcagcactg
     4261 ccgagagcat gagcaggaca agccggttgg taccgataga cacgtctagt atgcctcgta
     4321 aagttgctga ctgacgccgg acggcacagt tgcagttggt attggagtga ttgtcggtgt
     4381 tggcgttgga agatcacctt caacgaccat ctccgcaata ctgaaccagc ttcctgttcc
     4441 cgtgcccgga acgccttcgt tggagcgtgt gacatgaacg cgtacaccgt cggcaagcat
     4501 cccaacaaaa tgcgaatcct tagaccactc catatcattg tcattgttgg gcttgtctat
     4561 cacgttgatg atctgcaccc acccctcgtt gtctggatag cctggcatgt gaagggagac
     4621 agcgaaatcc cttggatcac tacagttgcc ggggttcccc tgaaaccaca ttttgacctt
     4681 gctcggaaca gcagacttgt tgaaccagac cgtattcacc gccgccgggc caggtgccat
     4741 tccccaacaa cagatcatgc cactgttatt gttgtcgtta agacctgacg cgggagtgct
     4801 gtctcccccc tgacagcaac cagcatcggc accgtttcgc ggatcggtaa gaactctctg
     4861 ttcagggatt tcatacaagg tgcatgtctt ccaagtcagg caggtctcac aggagtagtc
     4921 ggcaacagaa aactggttgg caacaaggca gagtatcacg ctcagtataa agacgcaaga
     4981 aagtctcttg aacacgagtc attctccttt ccagatcaat tcattctcaa ggtccccaac
     5041 ggattatgtc tccctcactg accaccccat tggtcggatc atagagaatc cagccaccac
     5101 tgtccgtgtg atcgggaccg taggaatcaa gtttccacag atacattttg tgaggccaaa
     5161 caccccaacc accgagcgga tcgcttgggt cccagttgcg tacaggtgtt ctagccatgg
     5221 gcaaagtagg tgtcatctgc cagttgtgac ctgatccgtc aaagtaatag acatagttgt
     5281 ttgcaatggg atcgttgcct gtggcaaacg gatccgttgc tggcaatctc ttgagatagc
     5341 cgaccggagt tgtcaagcgc cagagggttc tgagaattct ctgacgatgc tcaactggga
     5401 acacaagatt cggattgcca tgggtcaccc agtcatcggg gggatagttg ttctcatcaa
     5461 tgacatacgc ttccagccca gtgccaaggg agacaaactc ggatcgaact cgagcaacct
     5521 tcgcgcgcgt ctgggcctga agaaaattcg ggatggcaat ggcggcaagg ataccgatga
     5581 tggcaacaac gattaagagt tcgataagcg taaagcccca atgccatctc tttggatacg
     5641 agtgcgaagt ggacgttctt gaaaggcagc acatcttaac tcacctccaa ttctccgaga
     5701 agcaggtgat acgcctcata tctatcatcg tttaactggg tgcgtcaagc gagaactcgt
     5761 cctcaaatgg tttccactca agaaactatg gaagccggtt tcagaaaggc caaacgggac
     5821 ggttcgacgt ggatatgcca gaggtgtcga ggctcaagca attcaccatc aacctgtgcc
     5881 tgaatcggtt cttgtgaagc gatcgtcaga tgtctgaccc gatgaaaagt cacaatggaa
     5941 tgacggccgt gagttccgtt gaggcttgac ggaagacacc gaagaagcct gcaacggctg
     6001 cactgactga ccagacagac atccaacaaa ccgtcgtcgg gaagagcatt cggggtgagt
     6061 ctgaatcccc caccactgaa agctccattt cctatcgcca cgagtgaaaa
//
LOCUS       DAOQUC010000037         6104 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_14280_length_6104_cov_3.324516, whole genome shotgun sequence.
ACCESSION   DAOQUC010000037 DAOQUC010000000
VERSION     DAOQUC010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6104
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14280_length_6104_cov_3.324516"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(43..1839)
                     /locus_tag="PKH07_01160"
     CDS             complement(43..1839)
                     /locus_tag="PKH07_01160"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNT33587.1"
                     /translation="MVRRRRESRESAKDRKAISESQSDSVRQPLSSSWFDSPKAAILA
                     LLLLGFVAHWRVLGAGFVWDDYLIYENLYVRSLSGIWKIWTNLAVQPESDVRYWPLFY
                     STFWLDYVISGIHPVFSHGVNLLIHLANILLVWHVLKRLAFPGALLAAAIFAAHPVHS
                     ESVAWVIERKGLLSMLFFLLAFRAHLQFVNTKDVKTLGVAVLMLALSLLSKPVAVVFP
                     VVVVLWMYWKGIVFSKRNLLFTVPYFALSAVSGLLNVLSSRLYDPVSFGFTPAERLLI
                     VGRAFWFYVSKLIAPYSLAMIYERWSVDTGNFLQWLFPISAVLLFVLLWWARSRIGKE
                     PLIAFSLFFVILSPVLGFVDYSHMAYSFVANRYQYLASVPLIALFSAGILRWARARID
                     LHRSGFSVVLGFILIILCLLSWQQDATFKSMETLFRHNIKVNPNAAAAHDSLGLCLLQ
                     RGEPMQAQKHFQRALELEPHMLEAIVNLGLAFAHQDKADQAIEQYEKALAIDPDNAPA
                     LTNLGNIYARLGRYDESLRWFNRLVEIGKASPQILNSLAASYAYMGRFEEAVETVQKA
                     IDQAHQQGDVQMAIGLERILQLYRSGQTVPRQ"
     gene            complement(1934..4123)
                     /locus_tag="PKH07_01165"
     CDS             complement(1934..4123)
                     /locus_tag="PKH07_01165"
                     /inference="COORDINATES: protein motif:HMM:NF039707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6259 domain-containing protein"
                     /protein_id="HNT33588.1"
                     /translation="MSVKPDFLETMIMGFCVLVLIGALVSLRTATARAADKPTAQRIT
                     LADNSVCLTLGNKNDGFAIRSLFCSVRQREFIADALHPLWTLFVEKDGQESLEWTSIS
                     EVFKREAQYTRTRAKLIWRGTGDARGLVVLVSAVVADSRVTLKMSVENRSRKLSLMRV
                     RFPSMALCQIGESAEDDAFLRPEISGILAKAPLVKGLQSDAHYPGGWGPLQFVAHYDQ
                     DAGLYLGTHDPLACFKRFFSRKTDSGIEVSVEWPAESASVAGNDFKHPGEIVLSTFGG
                     DWFDASQIYRQWVSAKAKWWPQRRKYGRPDIPDWLKDICFWIRIRGVSDQLVEDAKRV
                     AEFMGVPTALHWYDWHVIPYDTDYPHYFPAKEGFAEGIAELQEAGIRVMPYINGRLWD
                     SEAPDFEQSGRAVATKDRKAECYVEIYKSGRKLVPVCPTQPLWQKIVKNATLRLFSPE
                     FNVDGVYIDQIAAANPKECYDATHGHPLCGGHWWNTEGYWPLLRSLRRSMSRYPDRFL
                     TSECNADPYAHLFDAYLTWHFQYNDMVPAFAAVYADKILTFGRAYGGTEPLAHRMRAA
                     QSLVFGEQIGWITTDVLDSQPDTAIYIRKCARIRYRLLDYLARGVMLRPPTVHGEIPD
                     VTADWAWFGKLDVTNSALQRGAWKAEEGSVAFVFANSSDEPISFTWDFSPEQYGLPNR
                     ALSVRKITEEGKSEEPNIQGQTSLKMHLEPRGIESYLVRAKKSVSRK"
     gene            complement(4218..5219)
                     /locus_tag="PKH07_01170"
     CDS             complement(4218..5219)
                     /locus_tag="PKH07_01170"
                     /inference="COORDINATES: protein motif:HMM:NF016764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HNT33589.1"
                     /translation="MEEPATAKPNQRMCPNCGQTIAPTYQGLYCPNCGKPLWDARPLQ
                     EQSQGQGDGAEIGVAPDLADGSDGADRGIPVFRVGQPSPPSEYHPYAAGPLPIRPLSH
                     AGTGGATAKFCIWEVRDQAGRIRAFLSTFFRSCLSPVSFFKQLPPKGGYSDALLFAIL
                     ANLFAAIVNVGWFALTRDIRAQFEKTLLADPWLGPMVERVLKEMPEMTLTRQMLPVVG
                     SPIYTIIWLLLFAVLFHLFLFLFAGARKGLQATFRCLAYASSVNLMTALFSPMCGGIL
                     WLFWMPIVVLIAFVQVHEIRLWRTLLAVVAPGLILVALLLFSFLWLALTIVAGMTPV"
     gene            complement(5243..>6104)
                     /locus_tag="PKH07_01175"
     CDS             complement(5243..>6104)
                     /locus_tag="PKH07_01175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33590.1"
                     /translation="VLALMLNLEEYVEHLDDFIKTIPWLKLFKYDNRSDQICLEPEYG
                     ASDLMVKFFASQTDRYLLLYTKSDNVDHLLDLPHGGHTIVNWSLSCDRVSREIERFTP
                     HVTARIEAARKCQQAGYTVRTRFSPIIPIADWREQYALTTRELLRQVKPDVVTLDVIG
                     WMSAGMLARCVNVSQFDRPYREFIEDIIRQGDPRRGKPYNPDGKHIFPTELRAPIYRH
                     MIDVIRQSNPEQRITICMETPEMWNLFGDELGMAPEDYVCCCGPTSVPGHPLLSACQQ
                     GHGSSKIPLL"
BASE COUNT         1546 a         1660 c         1630 g         1268 t
ORIGIN      
        1 gggacttggg acgctgttca cggatcagtt gaacaggtgt tcttactgcc tgggaacggt
       61 ttgacccgag cggtacaact gcagaatccg ttccaggccg atggccatct gcacatcacc
      121 ttgttggtgt gcttggtcta tcgccttttg aacggtctcg actgcctctt caaagcgacc
      181 catataagca taggaagcgg ccagtgagtt gagtatctgg ggcgaggctt taccgatttc
      241 caccagtctg ttgaaccatc tcagcgattc atcatagcgt cccaggcgag catagatgtt
      301 accgaggttg gtcagggcag gggcgttgtc tggatcgatt gcgagagcct tctcgtattg
      361 ttctatcgct tggtcggctt tgtcttgatg ggcaaaagca agccccagat ttacgatggc
      421 ttcaagcatg tgaggttcca gttcaagtgc cctctggaaa tgcttttgcg cttgcattgg
      481 ttctccacgc tgcaacaggc acagcccaag actgtcatgc gcggcggcgg cattcggatt
      541 gaccttgata ttgtgacgga agagtgtctc catgcttttg aacgtcgcat cctgctgcca
      601 ggaaagcaga caaagaatga tcaggataaa ccccaggaca accgaaaatc cggagcgatg
      661 aagatcgatt cgtgctcgtg cccatcgaag gatgccagcg ctgaacagcg cgatcaatgg
      721 aacactggca agatattggt agcggttggc aacgaaggag taggccatgt ggctgtagtc
      781 cacaaaacca agcacaggcg acaggatcac aaagaacaga gagaacgcaa tgagtggctc
      841 cttgccgatc cgtgacctcg cccaccagag aaggacaaac aacagcaccg cggagatggg
      901 aaacaaccac tgcaggaaat tgcctgtatc aacagaccag cgttcataga tcatcgccag
      961 ggaataaggc gcgatcagtt tgctgacata gaaccagaaa gcgcgcccaa cgatgagcaa
     1021 tcgttcggca ggagtgaatc cgaaactgac cggatcatag agtcgtgacg acagaacatt
     1081 cagaagcccc gagacggccg aaagagcaaa atagggaacg gtgaacaaca ggttgcgttt
     1141 cgaaaacacg atccccttcc agtacatcca cagcactacg acgacaggaa acacaacggc
     1201 aacgggcttg ctcaagagcg acagagccag catgagtaca gccacgccca gggtcttgac
     1261 gtcctttgtg ttgacaaact gaagatgagc tctgaaagcc agaaggaaga aaagcatcga
     1321 gagcagtccc ttgcgctcaa tgacccaggc gaccgactct gagtgaaccg gatgagcagc
     1381 aaagattgcc gcggccagca atgcacctgg aaaagcaagc ctcttgagca catgccacac
     1441 gagcagaatg tttgccagat gaatcagcaa attgactcca tgagagaaga ccggatggat
     1501 accggagatc acgtagtcca accaaaaagt ggaatagaac agaggccagt accgaacgtc
     1561 tgattccggt tgaaccgcca ggtttgtcca aatcttccag atacctgaaa gacttcggac
     1621 atagagattc tcatagatga gatagtcgtc ccagacaaag cctgcaccta gaacgcgcca
     1681 atgcgcgaca aaccccaaca gcagaagagc gagtatggca gcctttggtg agtcaaacca
     1741 agaagaggag agaggctgcc gaacggaatc ggactggctc tctgagatgg cctttcgatc
     1801 ttttgctgac tcgcgagact ctcgccttct tctaaccatc ggcttcttcg atcgatagaa
     1861 tcccgcttga cgagcgggca aattgcacat ggtacaggca tcgaacggcg aaaagtaagg
     1921 gtcttgccaa tgcttacttt ctggacactg acttctttgc ccgaaccaga taggactcga
     1981 tgcctcgagg ctccaagtgc atcttgagag aggtctgccc ttggatgttc ggttcttcgg
     2041 attttccctc ttccgttatc ttccggacac tgagagccct gttcgggagg ccatattgct
     2101 cgggactgaa atcccaggtg aaggagatcg gttcatctga agagttggcg aacacaaatg
     2161 cgacagagcc ttcttcggct ttccacgcgc cacgctgaag cgccgaattt gtcacatcca
     2221 acttaccgaa ccaagcccag tcagccgtaa cgtcagggat ctctccatga actgttgggg
     2281 gacgaagcat gacccctcgg gcaaggtaat caagaagccg gtaccggatt cgagcacatt
     2341 tgcgaatata gattgcggtg tcaggttgac tgtccaaaac gtccgtggtg atccatccga
     2401 tttgctcacc gaacaccagg gactgtgcgg cgcgcatccg atgcgcgaga ggctccgtgc
     2461 ccccgtatgc acgtccaaag gtgagaatct tgtctgcgta caccgcggcg aaagcaggca
     2521 ccatgtcgtt gtactgaaag tgccacgtca gataggcatc aaacaaatgc gcgtagggat
     2581 ctgcattgca ttcactggta agaaatcgat cgggatatcg gctcatggag cggcgaagac
     2641 tccgcaacag tggccaataa ccctccgtgt tccaccaatg tcccccacag agtggatgtc
     2701 cgtgcgtcgc gtcgtagcat tccttggggt tggctgcggc gatctgatca atgtagacgc
     2761 cgtccacatt gaactctggt gagaaaagcc gcaacgtcgc gttcttgact atcttctgcc
     2821 acaaaggttg tgttggacag acgggaacga gtttcctgcc ggatttgtag atctcaacgt
     2881 aacattccgc cttgcgatcc ttggtcgcta cagcacgtcc cgactgctcg aaatcgggcg
     2941 cctcagaatc ccagaggcga ccattgatgt acggcatgac ccgaatgccc gcctcctgca
     3001 actcagcaat gccttcagca aatccctctt tggcgggaaa gtaatgcgga tagtcggtat
     3061 cgtatgggat gacgtgccaa tcataccagt gcaaagcggt agggactccc atgaactcgg
     3121 caactctctt ggcatcttct accaattggt ccgagacccc tctgatcctg atccagaaac
     3181 agatatcctt gagccaatcg ggaatatcgg gacgaccata ctttcgacgt tgcggccacc
     3241 atttggcttt cgcagacacc cattgtctgt agatctgaga ggcgtcgaac caatcaccgc
     3301 caaacgtcga aagaacaatc tcaccgggat gtttgaaatc gttaccagcg acggaagcgc
     3361 tctccgcagg ccattcaacg gagacctcta ttccggaatc cgtcttgcgg gaaaagaatc
     3421 gcttgaaaca ggcgagagga tcatgtgttc ccagatacag tcccgcgtcc tgatcgtagt
     3481 gtgccacaaa ctgaagcggt ccccatccac ccggatagtg tgcatcgctc tgcaatccct
     3541 tgaccaaagg agctttggcg agaatgcctg atatctcggg tctgaggaaa gcgtcatctt
     3601 cggcgctttc accgatctgg cagagcgcca tgctgggaaa ccgaacgcgc atcaggctca
     3661 gtttcctgga gcgattctcc acagacatct tgagcgtaac tctcgaatcg gcaacaaccg
     3721 cgctgacgag cacgacaagc cctcgcgcgt caccagtgcc tcgccaaata agtttcgcgc
     3781 gcgtcctggt gtactgtgct tcgcgtttga agacttctga aatgcttgtc cactcaagac
     3841 tttcctggcc gtctttctcg acaaaaagag tccagagagg atgcagcgca tcagcgatga
     3901 actccctttg ccgaacgctg cagaaaagac ttctgatggc aaacccatcg ttcttgttgc
     3961 caagcgtcag gcaaacggag ttatctgcaa gcgtgatccg ctgtgccgtg ggcttgtccg
     4021 cggcacgagc cgttgcagta cggaggctga caagagcacc aatcagaacc aacacacaga
     4081 atcccatgat catcgtctcc aggaagtcgg gtttgacgga catcgcagtg aaggtctccc
     4141 tctttgcgtc ctcgtcaaat gcacgatgca ataccccgcc gtgggttcct tctctcaaag
     4201 catcggcgtg acgtgtgcta cacgggtgtc atccctgcaa cgatagtcaa cgccagccag
     4261 agaaacgaaa atagcaacaa cgccacaagg atcaaaccgg gcgcaacaac tgcaagcaaa
     4321 gtccgccaga gacgaatctc atgaacctgc acaaatgcaa tcagaacaac aatgggcatc
     4381 cagaacaacc aaagaatgcc cccacacatc ggcgagaaca acgcggtcat caggttcacg
     4441 ctgctggcgt aggcaagaca gcgaaacgtc gcctgaagac ctttccgcgc gcccgcgaac
     4501 aagaagagaa agaggtgaaa gagcaccgca aaaagcaata gccagatgat tgtgtagatt
     4561 ggagagccta cgaccggaag catctgcctg gtcagggtca tctcaggcat ctccttgagc
     4621 acccgttcta ccatggggcc gagccaggga tccgccagca gggtcttctc aaattgtgcg
     4681 cgaatatccc gcgtcaatgc aaaccagccc acgttgacga tcgccgcaaa aaggttcgcc
     4741 agaatcgcga acagcaacgc atcagaataa ccaccctttg ggggaagttg tttgaagaac
     4801 gaaacaggcg acagacagga tcggaagaac gttgagagga atgcgcgaat gcgaccagcc
     4861 tggtcacgaa cttcccagat gcagaacttc gcggtggctc cacctgtccc tgcatgggac
     4921 aggggtctga tcggcaaggg accggcagca tagggatgat attcgctggg agggcttggc
     4981 tgaccaacgc gaaatacagg gataccacgg tcagccccat cggaaccatc tgccaagtcg
     5041 ggcgcaactc cgatttccgc accgtctccc tgaccttgag attgctcctg tagggggcgc
     5101 gcatcccaga gcggtttgcc gcagttcgga cagtagagcc cctgataagt tggagcaatt
     5161 gtttggccac aattgggaca cattctctga tttggctttg ccgttgctgg ttcttccatc
     5221 tttgagcttc ctgatcgaaa gactataaca acggaatctt acttgatccg tgtccctgtt
     5281 ggcaagccga cagcagagga tgccctggaa ccgaagtcgg tccacaacaa cagacgtaat
     5341 cctctggagc catgccgagc tcatctccaa acaagttcca catttccggt gtctccatgc
     5401 aaatggtgat cctctgttcc ggattgctct gtctgatcac gtcaatcatg tgtcggtata
     5461 tgggcgctcg aagttctgtc gggaaaatat gcttgccgtc ggggttgtag ggtttgcctc
     5521 gtcgcgggtc gccttgacgg atgatgtcct caatgaattc ccgataggga cgatcgaact
     5581 gcgacacgtt cacacagcgc gcaagcattc cggcactcat ccatccgatc acgtccaagg
     5641 taacaacgtc cggtttgacc tgacgaagca gttccctcgt ggtcagcgcg tattgctccc
     5701 tccagtctgc aatggggatg attggcgaaa agcgggttct gactgtgtat cccgcctgct
     5761 ggcatttgcg agccgcctcg atacgggcgg ttacatgggg cgtaaaccgc tcgatctcgc
     5821 ggctgacccg atcgcaggag agcgaccagt tgacaatggt gtgcccgcca tggggaagat
     5881 cgagaagatg atccacgttg tcgctcttgg tgtagagcag caggtaccgg tcggtctggc
     5941 tggcgaaaaa cttcaccatc aaatcggatg cgccgtactc aggctccaga cagatctgat
     6001 cagatcgatt gtcgtacttg aagagtttca accaggggat ggtcttgatg aaatcatcca
     6061 gatgctcgac atactcctca agattcagca tcaaggcgag cact
//
LOCUS       DAOQUC010000038         6060 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_14399_length_6060_cov_3.072773, whole genome shotgun sequence.
ACCESSION   DAOQUC010000038 DAOQUC010000000
VERSION     DAOQUC010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6060
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14399_length_6060_cov_3.072773"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            93..347
                     /locus_tag="PKH07_01180"
                     /pseudo
     CDS             93..347
                     /locus_tag="PKH07_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009064199.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
     gene            complement(545..1369)
                     /locus_tag="PKH07_01185"
     CDS             complement(545..1369)
                     /locus_tag="PKH07_01185"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNT33591.1"
                     /translation="MKITVLVDNKAMPGLTTEHGFSLWIESGEKKILFDLGQGKALWS
                     NAERLGVDLSQADMLVFSHGHYDHTGGLSRLLSLKPNLDIYCEPRVLALRYSLSKDKV
                     ARDVSIPLDSSGAIATHIGEQIHWVQKPLWLSAEAGMTGEIPRVTSFEDTGGAFFRDA
                     AGTKPDHIEDDQAMWFKTPKGLVIVFGCCHSGMVNSINYILQIVGEQRIHAIIGGLHL
                     VNATKNRLTETCHVLKSLNVDEIIPCHCTGDAAVDFMRQALGDIVQPGCAGRIYSF"
     gene            complement(1433..1765)
                     /locus_tag="PKH07_01190"
     CDS             complement(1433..1765)
                     /locus_tag="PKH07_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005985479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HNT33592.1"
                     /translation="MRIAVPLADERLCMHFGHCETFALVDVVDGKIAKRTDVVPPPHE
                     PGVLPRWLAAQGVTMIIAGGMGQRAQGLFTENRIQVVVGAPSESPEKLIAAYLEGNLQ
                     TGENVCDH"
     gene            complement(1807..2697)
                     /locus_tag="PKH07_01195"
     CDS             complement(1807..2697)
                     /locus_tag="PKH07_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015774470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HNT33593.1"
                     /translation="MMNEENPKCSTCSDSDCSASKRRRNETDEEFADRQKLQSRLCRI
                     RHKIIVLSGKGGVGKSTVAVNLATSLMLLGKRVGLLDVDIHGPSIPTMLGLENEMLQG
                     SEEGLLPIELGDLKVMSLGFLLKNQDEAVIWRGPMKMGAIKQFLQDVAWGDLDYLIVD
                     SPPGTGDEPLSVCQLIGAVDGAVIVTTPQKVAAVDVRKSITFCHQLQVPVLGVVENMS
                     GFVCPKCGEVTQILSSGGGRKIAEDMNVPFLGSIPMDPRIAQASDDGRVFIHHYAETP
                     LAGIMRSIIQPIADLDGCCC"
     gene            complement(2694..4379)
                     /locus_tag="PKH07_01200"
     CDS             complement(2694..4379)
                     /locus_tag="PKH07_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015720112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNT33594.1"
                     /translation="MKVVIIGGVAAGPKTASKIIRLTPDADIAVVEKGSLLSYAGCGL
                     PYYVSGEVKEQKHLMETPAGAVRDSAFFQNVKNIKVHNNTEALEVDRAGKRVRVKSSK
                     TGEESWLSYDKLVLATGASPVIPRLSGIDKKNVFTLHGVHDAEGIKAILAEGKAQDVV
                     IIGGGLIGVETTEALAAHGCRVTVVEMLPQILNMLDWEIAQLVEQHLESKGVRVMTAT
                     KALALESRHGDGECVTHVQTDKGDLPADMVILAIGVRPNVTLARKAGLSIGEKTGALC
                     VDDHMRTSDPDIYAAGDCVECTDRLTGRPCFVPLGSTANKQGRVAAVNICGGNETFPG
                     ILGSTVCKVFDFCVARTGLTETGALSAGYEIVTSMAPGPDKAHYMPQSKPLLLKLVAD
                     KETGRLLGVQATGPGDGAKRIDVAATAISAGMTLDDIANLDLAYAPPYAPAMDNLITA
                     ANVARNKRDGLMVGISAQEVYRKIQDGADFFFLDVRSPQEYAEVRLPKSTLIPLGALR
                     KRLNEIPKDKEIVAFCKISLRGYEAALVLKAAGYDNVKVLDGGVAMWPYEKEK"
     gene            complement(4430..5311)
                     /locus_tag="PKH07_01205"
     CDS             complement(4430..5311)
                     /locus_tag="PKH07_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011735826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNT33595.1"
                     /translation="MIKELVVISGKGGTGKTSLTGSFAVLANQPVISDCDVDAADLHL
                     LLEPKIVQRRRFIAGQRAQIRPGHCTACGKCEELCRFDAIFYDGPGNGSVDKTFRVDP
                     IACEGCGVCARFCAEDAIVLQPTESGEWYISQTRCGTMVHAQLGIGAANSGRLVTLIR
                     KEAARLAEQENSSLILIDGPPGIGCPVIASVTGANVALVVTEPTVSGEHDLERVLALT
                     RHFDIDTAVCVNKWDINPEMTERIESSARQWGARIAGRVRYDTSLTSAQMQEKTAIET
                     HAPCADDIIGVWNNLNL"
     gene            complement(5308..5640)
                     /locus_tag="PKH07_01210"
     CDS             complement(5308..5640)
                     /locus_tag="PKH07_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011735810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGGC domain-containing protein"
                     /protein_id="HNT33596.1"
                     /translation="MKVGIVRCQQTEDICPGTKCLAYARDGKEAYQEMGNVEIVGFIS
                     CGGCPGKKAVARAVEMEKRGAEAVFLGSCMTRGLPWDYVCPHLDRIKESLKKKLKATT
                     RVIDWTHS"
     gene            complement(5694..>6060)
                     /locus_tag="PKH07_01215"
     CDS             complement(5694..>6060)
                     /locus_tag="PKH07_01215"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HNT33597.1"
                     /translation="GSRDSGGWMRDRAPDKTMVTELMIREAACRILALDKDFTEEDVR
                     KAYRRAAMRYHPDRNSDDPNAHQRFMLIKCAYELLSGGKPCDLLLTEISSWSCPPETT
                     GFRMDNPWGYFLWWQDKFF"
BASE COUNT         1379 a         1774 c         1470 g         1437 t
ORIGIN      
        1 tgtgtgcgac tgccagaatc agaatctccg gttccctctc ctggttgtgg gagaaggttg
       61 ttgtgagggg tttgtcagga ggaacgagca gcatggcggg tacggcactt ggactgatcg
      121 aaacacgagg cttggttgca gcccttgagg catcggatgc cgcggcaaaa gcatcggacg
      181 ttgtcgtcaa ggagatcgag attccctccg ctggcgtatg caccgtcaag attgtgggag
      241 aagttgccga cgttgcttct gccgttgacg ctgcggtcgc cgcggcgggt cgagtcggag
      301 aagtcctggc tcaccatgtt atcccgaggc ccgattcgga attggagaat ttcctctacg
      361 agaagaagat tgtgttgagt gagtcttccg gttctgtcaa tgccttcatc cgcgctactg
      421 cagatgagga caagggggac aagaagaagg ccacaaaaaa gtaggaacaa attcccaaca
      481 gcatcgtttt tcgatgagtg cggacactcg actctgactc tggtcagtag aggacagggt
      541 gctctcagaa tgagtatatc cgccccgcac aacctggctg aacaatatca cccaaagcct
      601 gacgcatgaa gtctaccgct gcatcgccgg tgcagtggca gggtatgatc tcatccacgt
      661 tcagactctt cagcacatgg cacgtctctg tcaggcggtt ctttgttgca ttgacaaggt
      721 gcaggccacc gataatggcg tgaattctct gttcgcccac gatctgaagg atgtagttga
      781 tggagttcac cattcccgag tgacagcacc caaagacaat gaccaagccc tttggcgtct
      841 tgaaccacat cgcttgatcg tcttcaatgt gatccggttt cgtgccagct gcatcgcgga
      901 aaaacgctcc accggtatct tcaaatgaag tgactcgtgg aatctctccc gtcatgcctg
      961 cctcggcgga aagccagagc ggtttttgaa cccagtgaat ctgttctcca atatgtgttg
     1021 cgattgcacc ggaagaatcc agagggatgc tgacgtcacg ggcgactttg tccttcgaga
     1081 gagaatatcg aagagccaga actcttggtt cacagtagat gtcaagattg ggcttgagag
     1141 acagaagtct ggaaagtcct ccggtgtgat cgtagtgacc atggctgaag acaagcatat
     1201 ccgcttggct caggtccaca ccaagccgct ctgcattgct ccaaagggct ttgccctgac
     1261 ccagatcaaa gaggatcttc ttctctccac tctcgatcca gagagaaaag ccatgttccg
     1321 tggtgagccc aggcatggct ttgttgtcaa cgagaactgt gatcttcact actgcctctt
     1381 gaccaacgcc gaacgcactt aagagagtac cttttcaatt gtctgatcac actcagtggt
     1441 cacagacatt ctcacctgtc tggaggtttc cctcaagata cgccgcaatg agtttctccg
     1501 gagattcgga tggagcaccg actacgacct gaatcctgtt ttccgtgaac aggccttgcg
     1561 ctcgctgacc catgccaccg gcaatgatca tcgtcacccc ctgcgccgcc aaccacctgg
     1621 gcaagactcc aggttcgtgt ggcgggggaa ccacgtccgt tctcttggcg atcttgccat
     1681 ctacgacatc caccagggca aaagtctcgc agtgtccaaa gtgcatgcag agtctctcgt
     1741 ctgccaaggg aaccgcaatt ctcatcttga tctcctctat ctattcagaa cctttgttca
     1801 ttcttctcaa caacagcatc catccaaatc cgctatcggt tggatgatgc tccgcattat
     1861 tcctgccaag ggtgtctcgg cgtagtgatg gatgaaaacg cgtccgtcat cagatgcctg
     1921 ggcgatcctt ggatccatgg ggatagatcc aagaaagggc acattcatgt cctccgcgat
     1981 cttccgaccg ccaccggaac tgagtatctg tgtcacctca ccacatttcg ggcaaacgaa
     2041 accactcatg ttctccacca caccaagcac aggcacctgc aactgatggc aaaacgttat
     2101 tgatttccga acgtccaccg cagccacttt ttgtggcgtc gtgacaatga cggctccgtc
     2161 cactgcgcca atcaactggc agacagacaa gggctcatcc ccggtgccag gaggggaatc
     2221 cacgatcaga taatccaaat ccccccaggc aacatcctga agaaactgct tgatcgcacc
     2281 catcttcatg gggccgcgcc agataactgc ttcatcctga ttcttcagca gaaagccgag
     2341 cgacatcacc ttcaaatctc ccagttctat cggaagcagt ccttcctccg acccttgaag
     2401 catctcgttc tcaagaccca gcattgtcgg aatacttgga ccatgaatgt ccacatcaag
     2461 caggccaacc cgctttccca agagcatcaa cgaggtcgcc agattgaccg caacggtgct
     2521 cttaccaacc cctccctttc cagagaggac gatgatcttg tgccgaatgc ggcagagtcg
     2581 cgactgaagt ttctgtcgat cggcaaactc ctcatctgtt tcattccgcc gacgcttcga
     2641 tgcagagcag tctgaatcac tgcacgtact gcatttcgga ttctcttcgt tcatcatttc
     2701 tccttttcat aaggccacat ggcgacaccg ccatccagaa ccttgacatt gtcatatccc
     2761 gctgccttga gcaccaaggc cgcctcataa cctctcagag agattttgca gaaggcgaca
     2821 atttccttgt ctttgggaat ctcgttcagc ctcttccgaa gggcgccaag cggaatgagg
     2881 gtagatttcg gaagacggac ttcggcatac tcctgcggag accgaacgtc caggaaaaag
     2941 aaatccgctc cgtcctgaat ctttcggtag acttcctggg ctgagatgcc gaccataaga
     3001 ccatcacgct tgttgcgtgc aacattggcc gcagtgatca agttgtccat ggcaggtgca
     3061 tacggaggag cgtacgcaag atcgagattc gcgatatcat ccagagtcat tccagcggag
     3121 atagctgttg ctgcaacgtc aattctctta gctccatcac ccggtcctgt tgcctgaact
     3181 cccaaaagcc tgcctgtctc cttgtccgcg acgagtttca gcagcaaggg tttggactgc
     3241 ggcatataat gggctttgtc cggtcctgga gccatggatg tgacaatctc ataaccggcg
     3301 gacaaggctc ctgtttcggt cagaccggtt cgggcaacac agaagtcaaa cactttgcag
     3361 acagtgctcc caagaatgcc agggaaggtc tcattgcccc cgcagatatt cactgcagca
     3421 actcggcctt gtttgttcgc cgtggaacca agtggaacaa aacagggacg gccggtcagg
     3481 cggtcagtgc actcaacaca gtcaccagcc gcatagatgt cggggtcaga agtccgcatg
     3541 tgatcatcaa cacagagggc tcccgttttc tccccaatgg aaagtccagc cttcctggcc
     3601 agtgtgacat tgggacgaac gccgatcgcc agaatgacca tgtctgccgg caaatccccc
     3661 ttgtccgtct gaacatgtgt cacgcattcc ccgtcgccat ggcgactctc cagggcgaga
     3721 gccttggtgg cggtcatcac gcgcacccct ttggattcaa gatgctgctc caccagttgc
     3781 gcaatctccc agtcaagcat gttgagaatc tgaggaagca tctccacgac ggtcacccga
     3841 catccgtgtg cggcgagtgc ttccgtggtc tccacaccga tcagcccacc tccgatgata
     3901 accacatcct gggccttgcc ttcagcgagg atcgccttga ttccctctgc atcatgaaca
     3961 ccgtgcaagg tgaagacgtt cttcttgtca atgcccgaga gacgtgggat gaccggagaa
     4021 gcgcccgtgg ccaagaccag tttgtcgtaa gaaagccagg attcctctcc cgtcttggaa
     4081 gacttcacac gaactcgctt gccggctctg tctacctcta aggcttctgt gttgttgtga
     4141 actttgatgt ttttgacatt ctggaagaat gccgagtcgc gaacagctcc ggcaggggtc
     4201 tccatcaagt gcttttgttc tttcacctcg ccagacacgt agtagggaag accgcagcct
     4261 gcataagaga gcagactgcc tttttccaca actgcgatat ccgcatccgg tgtcaagcgg
     4321 ataatcttgg atgcagtctt aggtcccgca gcaacaccac caatgatgac aaccttcatg
     4381 atttctcctt ttctcgctcc ctcaagtgtt tcccaccgtc ttcgtctccc tacaggttga
     4441 gattgttcca cactccaatg atatcatccg cacaaggagc atgcgtttca atcgctgtct
     4501 tctcctgcat ttgagcagaa gtgagcgaag tatcatagcg aacacggcct gcgatgcgtg
     4561 ctccccattg gcgtgcactg ctctcaatcc gttctgtcat ctccggattg atgtcccatt
     4621 tgttgacaca cacagcggtg tcaatgtcga agtggcgcgt cagtgccaga acacgttcca
     4681 gatcatgctc tcccgacacg gttggctctg tgaccacaag tgccacattc gcgccagtca
     4741 ctgaggcaat caccgggcaa ccgatccccg gcggtccatc tatgagaatc agcgaactgt
     4801 tctcctgttc tgcaagccgt gcggcctctt tgcgtatcaa agtcaccagc ctgcctgaat
     4861 tcgctgcccc gattcccagc tgtgcatgaa ccattgttcc gcaacgagtc tgagaaatgt
     4921 accattcgcc gctctcagtt ggctgcagga caattgcatc ctctgcgcaa aaacgcgcgc
     4981 acacgccaca gccctcacaa gcaataggat cgacccgaaa ggtcttgtcc accgagccgt
     5041 ttccgggccc gtcatagaag attgcatcga accgacacaa ctcctcacac ttaccacagg
     5101 cggtacaatg cccagggcgt atctgagctc gttgcccagc gatgaaacgc cgtcgttgca
     5161 cgattttcgg ttccaacaac aagtgcagat ccgccgcgtc cacatcacag tccgatatca
     5221 caggctgatt tgccagaacg gcgaaagaac cagtcagact ggtcttgccc gttccacctt
     5281 ttccactgat gacaacaagt tctttgatca tgaatgcgtc caatcgataa ctcgtgtcgt
     5341 tgccttgagt ttcttcttaa gactctcctt tatcctgtcc agatggggac aaacataatc
     5401 ccatggaagt ccacgagtca tgcaggaacc gagaaaaact gcttccgccc ctcgtttttc
     5461 catctccacg gcacgtgcga cggccttttt gcctggacat ccgccgcacg agataaagcc
     5521 tacaatctcg acattcccca tctcctggta tgcttccttg ccatctcggg cataggcaag
     5581 gcattttgtc ccgggacaga tatcctccgt ttgctgacac cgcacaattc caactttcat
     5641 ccgactcttc ctctctcact gtgatgatag gactctccgg ggtgagtgtc ttctcagaaa
     5701 aacttatctt gccaccagag gaaatatccc caaggattat ccattcggaa gcccgtcgtt
     5761 tccggaggac aggaccaact ggatatctcc gtcagtaaca ggtcgcaggg cttgccgccg
     5821 ctcaggagtt cgtaggcgca cttgatgagc atgaacctct gatgtgcatt agggtcatcc
     5881 gaatttcggt cggggtgata cctcatggct gcacgtctat atgccttcct gacatcctct
     5941 tcggtgaaat ccttgtccaa tgcaaggatc ctgcaagcgg cctcgcgaat catcaactct
     6001 gtcaccattg tcttatctgg tgctctatct ctcatccatc ctccgctgtc tctgctgcca
//
LOCUS       DAOQUC010000039         6053 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_14421_length_6053_cov_2.976159, whole genome shotgun sequence.
ACCESSION   DAOQUC010000039 DAOQUC010000000
VERSION     DAOQUC010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6053
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14421_length_6053_cov_2.976159"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="PKH07_01220"
     CDS             complement(<1..210)
                     /locus_tag="PKH07_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HNT33598.1"
                     /translation="MDSTLTLWSLAIASFTIALSGALMPGPVLAVTISLAPRKGFWAG
                     PLVILGHGILEALLVAAFAGGAQSGA"
     gene            complement(307..1572)
                     /locus_tag="PKH07_01225"
     CDS             complement(307..1572)
                     /locus_tag="PKH07_01225"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNT33599.1"
                     /translation="MLFRALRSRNYRLYFIGHGLSLIGTWMHSIALQWLVYRLTGSAR
                     LLGVVTFSTQICGLVLAPFAGVIVERMDRRRLLMVTQSLAMTLALMLATLVFTETIAV
                     WHIIVFSICLGSVNAFDITARQSFVVHMVEGKEDLANAIALNSMMFNGARFLGPAVAG
                     VLIAKVGEGFCFLINAISYISILIALALMRVQPWEHVRGKRLWDDLVEGFEYAFHFQS
                     LRTVFTLLALVSLFALPYTVLLPIFANDILQGGAKGYGWLVSSIGVGALCGAVFLASR
                     KTVVGLEKVIAHGPLLLGLAMISFSWSRMIFLSMGILLFMGFGMMVHMASCNTVVQTI
                     VDEKMRSRVMSIHMMTWTGLAPFGGLLVGDMAERTSPPITLTVAGVVCILGFLFFRKR
                     MLQQRHVIETAFASHKTESVLPQMPNRVG"
     gene            1871..2197
                     /locus_tag="PKH07_01230"
     CDS             1871..2197
                     /locus_tag="PKH07_01230"
                     /inference="COORDINATES: protein motif:HMM:NF020473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HNT33600.1"
                     /translation="MVEENNNTSSEIHGPRKVSFAEFLMLLSTQTMMQLGMIPNPFTK
                     KSRVDLVGAQTTIEAIHMLRDKTKGNLTQEEAKMLDSALHNLQLRFVEVVQEAEKTQH
                     KSEEKE"
     gene            2232..2669
                     /gene="dtd"
                     /locus_tag="PKH07_01235"
     CDS             2232..2669
                     /gene="dtd"
                     /locus_tag="PKH07_01235"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HNT33601.1"
                     /translation="MRVVIQRVSQASVEVDEKTVGAIGAGLVVLLAEGHEDTESTADW
                     MAEKITSLRIFNDADGKMNRSLGETEGSLLVISQFTLYGDCIKGRRPSFTDSALPDKA
                     ILLYEYFVTRCKQLGFRVEQGVFGAMMLVHIDNDGPVTFVLER"
     gene            2683..3816
                     /locus_tag="PKH07_01240"
     CDS             2683..3816
                     /locus_tag="PKH07_01240"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNT33602.1"
                     /translation="MQNNSPTSANLPSVSILIPTFNGRHLLEECLPTLAHLDYPRDLL
                     QTVVFDNGSSDGTSDFLRSKYPDVVILRSEENLFFAPAINRAVQEAQTEVVAFLNNDM
                     KVQSDWLEKLVNPLCTGQADCVASTILDWEGRNYQFVGGGINFLGQGFEHGGSLETTT
                     PEAKPLLFACGGAMAVRRSLFLEAGAFDEDYRLLYEDVDFGWRLNLMGYRVVLSPLAR
                     VFHRAHASVSRMDVMNRVRILERNALFTLIKNYQPSVLENVLPVALLLAERRAMVFTD
                     VANKVPLHEKITRLVTRRGRFKHKGEAIHAALDDVACHYPVLLRKRTSIQDRRRVSDD
                     RIFPLFCDSMRVWAYENDDYRILEQKGYPELLSQLCSHAMDSA"
     gene            3977..5782
                     /locus_tag="PKH07_01245"
     CDS             3977..5782
                     /locus_tag="PKH07_01245"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HNT33603.1"
                     /translation="MRVQLCWDPKTTPAELRTMLKTLGEEYPITQEGAQGVNVAFERI
                     ADPGQCCVEMSGQTALVRYNKPCQAARAVGALLGGLVKKGQIYSESTPFGMLGVMIDC
                     SRNAVMTVDHLKIWMRRLALLGYNMLMLYTEDTYELPEEPYFGYQRGAYTKAEIKAVD
                     AYAAKLNIETIPCIQTLGHLERILRHRAYGVVRDTPSVMLVGEQKTYDLIEKMIRHWT
                     DCCRTKRIHIGMDETHNLGRGRYLDLNKYRHGFELFNEHLGKVVEICHRYERKPMIWS
                     DMYFRLGAPSGSYYDLNSVIPPRVVNKIPKDVELVYWDYYHNNVAFYLDWIARHRKMG
                     KEPLMGSGIWTWSRYWYDHRITEANAGACIDACYEAKLNEVFFTQWGDNGAYCDHDSA
                     FAGMVWCADKSYGKKTPDAAVLEKRFGAVCGGSYAAHILAGEIHGAIEGFHPDLWDDP
                     IFETHFRTFCQNDPRRMAKIASGFETLAKKIQKHANDRATGDLLYASRLIRAMADRLT
                     FAAQLLSSYRKKDKTVLRKMLRTVPKLIATFEQLEGSFRDMWLSHNKPEGLETIQARF
                     GMIQARYRELGRRLKEYLEGKVGCISELDCSCPPK"
     gene            complement(5861..>6053)
                     /locus_tag="PKH07_01250"
     CDS             complement(5861..>6053)
                     /locus_tag="PKH07_01250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33604.1"
                     /translation="HLEPNIGIGLWDRYNLREEEMERRMSDAENRPFNWSNVGKSDRV
                     VLRNFAIAGRQYLEANMKR"
BASE COUNT         1533 a         1565 c         1630 g         1325 t
ORIGIN      
        1 cgcaccggac tgggcaccac cagcgaaggc ggcgaccaga agtgcttcca gaatcccatg
       61 tcccaagatg accagagggc cagcccaaaa tccttttctg ggtgccagag agatcgtcac
      121 agccaacaca ggccccggca tcaacgcccc actgagagcg atggtgaatg aggcaatggc
      181 cagactccac aaggtcagtg ttgaatccat agtgtcacgt gtctacaaca tcatcgttcg
      241 ggggtcaaga acgcgtccct gtcggcagcc aaacaaaaac acgcgttgca cagccgaccg
      301 gatccttcat cccacccgat tcggcatttg cggcaagacg gactccgtct tatgggaggc
      361 aaaggccgtc tcgatcacat gtcgttgctg aagcatcctc tttcgaaaga atagaaagcc
      421 cagaatgcag acaacgccgg ccacagtcag cgtgatgggt ggagaggtac gttctgccat
      481 atccccgacc agaagtcccc cgaaaggggc aagccccgtc catgtcatca tgtgaatgct
      541 catgacccga cttctcatct tctcatcaac aatcgtctgc acgaccgtat tacaggatgc
      601 catgtgaacc atcatcccaa aacccatgaa cagcagaata cccatggaga gaaatatcat
      661 ccgcgaccag gaaaatgaga tcatggcaag tccaagaagc agcgggccgt gagcaatcac
      721 tttctccaac ccaaccacag tctttcgtga ggcaaggaag acggcaccac aaagcgctcc
      781 aactccgatt gaagacacca accacccata gcccttcgca ccaccctgaa gaatgtcgtt
      841 ggcgaagatg ggcaggagca cggtataggg cagagcgaaa aggctgacca gcgcaagcag
      901 tgtgaaaacc gtgcggaggg attgaaagtg aaacgcatac tcgaagcctt ccacgagatc
      961 atcccacaag cgctttcctc ggacgtgctc ccaaggctga acacgcatca gcgcaagcgc
     1021 aatcagaatt gagatgtagc tgatcgcgtt gatcagaaaa cagaaacctt cccccacctt
     1081 ggcgatgagc acacccgcaa cggcgggacc aagaaatctg gctccgttga acatcatcga
     1141 gttcagcgca atggcgttgg ccaaatcctc tttgccttca accatgtgta ccacgaaaga
     1201 ctgccgggcg gtgatgtcaa atgcgttgac actccccagg cagatactga agacaataat
     1261 gtgccagact gcaattgttt cagtgaaaac gagcgttgcc agcatcaggg ccagcgtcat
     1321 ggcaaggctc tgagtgacca tgaggagtcg ccgtcgatcc atgcgttcga cgatgactcc
     1381 cgcaaaagga gccagcacca aaccgcatat ctgtgtcgag aaggtgacca cacccagaag
     1441 tctggctgac cccgtaagcc ggtagaccag ccattgcagc gcaatgctgt gcatccaggt
     1501 gccgatgaga gataaaccgt gtccgataaa atagagtctg taatttcttg aacgcagcgc
     1561 gcgaaaaagc attctctttg agcctgattc ccagagacaa cagtgtcatc caacctgctg
     1621 gcgcagaaaa gccgcggtgt tctccgcggc tgagcgtcaa cagaaagccc tggtcaaacg
     1681 taacatagaa agaatctagc acaacttccc aaaatgcaga agaggaacaa tgcgcgatcg
     1741 tcataactcc tgtctcttgg ttagtgctga gtttgtccaa gttgacagct gtgtaatagt
     1801 attggtattt tgagcaagca ttcaaagttg ccaagccctc tgaattgtca gtcaggttgg
     1861 gaggtacgac atggtagagg agaacaacaa tacatcttcg gaaatccacg gcccgcgcaa
     1921 ggtcagtttt gcagagttct tgatgcttct cagcacccag accatgatgc agttgggaat
     1981 gattcccaac cccttcacca agaagtcacg tgttgatctg gtgggagctc agacgacgat
     2041 cgaggccatc cacatgcttc gagacaagac caagggaaat ctcacccaag aggaagcaaa
     2101 gatgcttgac agcgcactgc acaatctgca actgcgtttt gtcgaagtgg tgcaggaagc
     2161 cgagaaaacc caacacaagt ccgaggaaaa agaatagttc ctgtgcattg gggaacgtcc
     2221 tcagcaacaa tatgcgcgtt gtcatacaga gagtgagtca ggcatctgtc gaagtggatg
     2281 agaagaccgt cggcgctatc ggagccggtt tggttgtgct tctcgctgaa gggcatgagg
     2341 acacagaatc aactgccgac tggatggctg agaagataac aagcctgaga atcttcaatg
     2401 acgctgatgg caagatgaac cgctcgctcg gtgaaacgga gggttctctg ctggtcatat
     2461 cgcagttcac gctctacggc gattgcatca agggaagacg cccaagtttc accgattccg
     2521 ctttgccaga taaggcaata ctactctatg agtatttcgt tacccgatgc aagcagctgg
     2581 ggtttcgtgt tgagcagggc gtgtttggag cgatgatgct tgttcatatt gacaacgacg
     2641 ggcccgtaac ttttgtcctg gagcgctgag aagggatatg caatgcagaa caactcacca
     2701 acttcagcca acctgccaag cgtctccatt ctgatcccaa ccttcaacgg caggcatctt
     2761 cttgaggaat gtctcccaac tctggctcat ttggattacc cgcgcgatct gcttcagact
     2821 gtggtctttg acaacggttc ttcagatggg acatctgatt tcctgcgcag caagtatccc
     2881 gacgtggtca ttctgcgtag cgaggagaat ctcttttttg cgccagcgat caaccgtgcc
     2941 gtgcaagaag cccagacaga ggtggttgct tttctcaaca atgatatgaa ggtgcagtcc
     3001 gactggcttg agaaactcgt aaacccgctc tgcaccggac aggcagactg cgtggcatcc
     3061 acaatcttgg attgggaagg aagaaactat cagtttgtcg gcggcgggat taactttctg
     3121 ggacaaggct ttgagcatgg gggaagtctg gaaacaacaa caccggaagc aaagccgctg
     3181 ctgttcgcct gtggtggcgc tatggctgtt cgcagaagtt tgtttcttga ggcgggagcc
     3241 tttgatgagg attaccggtt gctctatgag gacgtggatt tcggatggag actgaacctg
     3301 atgggctacc gagttgtact cagtccgttg gctcgcgtct ttcatcgggc acacgcttcg
     3361 gtgtctcgta tggatgtgat gaatcgggtc aggattcttg aacgcaatgc ccttttcacc
     3421 ctcatcaaga actatcagcc ttccgtactg gaaaatgtgt tgccggtcgc cttactcttg
     3481 gcggagcgtc gggcgatggt cttcacggat gtagcgaaca aagtgcccct tcatgagaag
     3541 ataacccgcc tcgtgacacg acgaggtaga ttcaagcaca agggtgaggc aattcatgca
     3601 gcgctggatg acgtggcatg ccactatcca gtccttctgc gcaagcgtac atccatccag
     3661 gacagacgtc gagtaagcga cgaccgcatc ttcccgctgt tctgtgactc aatgcgagtc
     3721 tgggcttacg aaaacgacga ctacaggatt ctcgaacaaa agggatatcc cgaactcctg
     3781 tcgcaacttt gctcgcatgc catggactcc gcttagcgca tccgattcca tctctcgcgg
     3841 aaagtcagcc tgccatgggc aacttgcaac tctcaaatgc tttggggttg ccttgagcca
     3901 aaggatttag tacggttcca aaaggtgagc cgttaagtaa cggtggatgt gattctggta
     3961 tgtcaggaga ttgttgatga gagttcaatt gtgctgggat ccgaagacaa cgcctgccga
     4021 gttgagaacc atgctgaaga cactcggcga ggagtacccg attacgcagg aaggtgctca
     4081 gggcgttaat gttgcctttg agaggattgc tgatccaggg cagtgttgtg tcgagatgag
     4141 tggacaaact gcccttgtcc gctacaacaa gccgtgtcag gcagctcgcg cagtcggagc
     4201 gcttctcggt ggtcttgtta agaagggaca gatctacagt gaatccacac cctttggaat
     4261 gcttggcgtc atgattgact gttcccgaaa cgccgtcatg accgtggatc atctcaagat
     4321 ttggatgagg cgtctggcgc tgctgggcta caacatgctc atgctctaca ccgaggatac
     4381 ctacgagttg ccagaggagc cctactttgg ctaccaacgc ggcgcttata caaaggctga
     4441 gatcaaagcc gtagatgcct acgctgcaaa actgaatatc gagacgattc cctgcattca
     4501 aacgctggga catctggaga ggattcttcg gcatcgtgcc tatggtgtgg ttcgcgacac
     4561 gccttctgtt atgctcgtgg gcgagcagaa aacctatgac ctgatcgaga agatgatccg
     4621 tcactggaca gactgctgtc gcaccaagcg cattcatatc ggtatggacg aaacgcacaa
     4681 tctcgggaga ggccgatacc tcgatctgaa caagtatcgc cacggctttg aattgttcaa
     4741 tgagcacctc ggcaaagtgg ttgagatttg ccacaggtac gagagaaagc cgatgatctg
     4801 gtcagacatg tatttccgac tgggggctcc atcgggatca tactatgatc tgaattccgt
     4861 catcccgcct cgtgtggtca acaagattcc caaagacgtg gagttggttt actgggatta
     4921 ctaccacaac aatgttgcct tttacctgga ctggattgcc cgccatcgaa agatgggcaa
     4981 ggaaccgttg atgggttcgg gcatttggac gtggagccga tactggtacg atcaccgaat
     5041 caccgaggcc aatgccggag catgcattga tgcctgctat gaggcgaaat tgaacgaggt
     5101 cttcttcact cagtggggag acaatggcgc gtattgtgac cacgattcgg cattcgcagg
     5161 gatggtctgg tgtgcagaca agtcctatgg taaaaaaact cccgatgcag cagtgctgga
     5221 aaagcgcttt ggggcggtct gcggtgggag ttatgccgcg catatccttg cgggcgagat
     5281 tcatggagcc atcgaaggct tccatcccga tctctgggac gatccgattt ttgaaactca
     5341 cttcagaacg ttctgtcaga acgatccccg tcggatggcc aagattgctt cgggtttcga
     5401 gacattggca aagaagatcc agaaacacgc caacgaccgc gcaacgggcg acttgttgta
     5461 tgcctctcga ctgattcggg caatggccga tcgcttgacg tttgcggcac aacttctttc
     5521 gtcctatcga aagaaggaca agaccgtgct tcgcaagatg cttcgaacag ttcccaaatt
     5581 gattgcgacc ttcgagcaac ttgagggatc cttcagagac atgtggctgt cccacaacaa
     5641 gcccgagggg ctggagacca ttcaagcacg gtttgggatg attcaagcgc gctatcgcga
     5701 gctcggcaga agactcaagg agtatctgga aggcaaggtt ggctgcatct ctgagctgga
     5761 ttgttcctgc ccacccaaat agaagaggaa cacaaggaaa caataacaac ggggcgctcc
     5821 ccatagaggg agcgccccgt ttgatttgat gtcagtgtga ctaacgcttc atgttcgctt
     5881 cgaggtattg acgaccggcg atagcgaagt tccgaagtac gactcgatcg ctcttgccca
     5941 cgtttgacca gttgaacggt cggttctcgg catcgctcat gcgtcgctcc atctcctctt
     6001 ccctgaggtt gtagcgatcc cagagaccaa ttccgatatt cggctccaga tgg
//
LOCUS       DAOQUC010000040         6011 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_14553_length_6011_cov_3.235393, whole genome shotgun sequence.
ACCESSION   DAOQUC010000040 DAOQUC010000000
VERSION     DAOQUC010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6011
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14553_length_6011_cov_3.235393"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="PKH07_01255"
     CDS             complement(<1..261)
                     /locus_tag="PKH07_01255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33605.1"
                     /translation="MRVNKRQWSGCKIPLGVLRLLILLTACTVGLSVPMVWAQEQTPS
                     ESVGDESEVQVQAQEPNQQLEAEEGLESIEPELSPSSVVSSGM"
     gene            complement(258..806)
                     /locus_tag="PKH07_01260"
     CDS             complement(258..806)
                     /locus_tag="PKH07_01260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33606.1"
                     /translation="MRTGGTMRRWISLLIFVFALGFSFVRVYGVVGGTGEKTPAPKAA
                     AGTSESRFLYDPEGLKDPFVPLIVPTEAPTPTPMPATPTPAGPTPTPTPTPVRLPPLS
                     VQVIMSTDQGGRLAVINGRLAGPGDFVDDVEVLDIHQHEVLVLYMNKEFRVSPAEDFE
                     KSEQETTSSQFEAKTKRKGRRR"
     gene            complement(859..1650)
                     /gene="pilO"
                     /locus_tag="PKH07_01265"
     CDS             complement(859..1650)
                     /gene="pilO"
                     /locus_tag="PKH07_01265"
                     /inference="COORDINATES: protein motif:HMM:NF016253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HNT33607.1"
                     /translation="MALTEKDKKVLQIVGVIIPVVILFSIFLPRFQIGSTPKPAVTIA
                     QTVQDTALETTDEAQTAEAGDESGTPVKKPGAKKPAPVQKPPLKIMGLKELYGEYKKV
                     TEQAAKIQQEVEDKERQVKKKADILAEVGDLERKIELWESRLPDSPRLDGLMAELNTL
                     AQVAGQKYVSMKMQPFREKSSHVELPLEIKLVTDYHALGNFINMTEYSERFSKVDDLS
                     IQGDPEFPGRINVGLVFSTFSFVAESENKDEEGEQTPAASGAGKS"
     gene            complement(1669..2385)
                     /locus_tag="PKH07_01270"
     CDS             complement(1669..2385)
                     /locus_tag="PKH07_01270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33608.1"
                     /translation="MITVNLLPHERRKARSPMMAQYLAIAGTAVVVGLVIMGTVYANM
                     QLRNQEQRVETLTLRSAELDVELEEVRALENKKALLSSKERVIKILAGQRLNWAPKLF
                     ELASLVPNGVWLESMYIKGDRVKKTRKEEVPGSKDAQGKPKFRQKTYYVIQKRLVIEA
                     ITDDLTNKAALVGQFISNINKNASFSRDFSNLGGVEAREEHWVLNDDTSPLVWRFKIS
                     MDINTRGGAEEEIGPARGRG"
     gene            complement(2414..3631)
                     /gene="pilM"
                     /locus_tag="PKH07_01275"
     CDS             complement(2414..3631)
                     /gene="pilM"
                     /locus_tag="PKH07_01275"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HNT33609.1"
                     /translation="MERLFGRSRPALGLDIGSHSVKLVELEKTGDQYELKRWVVHPLR
                     DEAAEEKALARSREAILAALKDIYSTHGIQNRRVVTAVSGESVIVRIITIPFIPNQDE
                     QMLQLSIQTEARDFIPFDMADVAFDFQLLGISERSGEQGQVQEVLIVAVKKDLVDSHV
                     HLLREAQLEPCIVDVGAFALVNAYAECSNQISGESAALVNIGAEITSIAIVKDNATRF
                     TRDLAIAGRNLTNALMSYLGKTREEAERIKAETGLGSRKGESESLSLTIEGNQYAQPD
                     IVSDVTKALEGLGLGDEPVMGATDSGPADQGRVAEVCEQILGDITSEVKRSLLFYENQ
                     LDGEPIGRIILSGGTAMLPNLCPFFEETLGVKTEIIRPLDKINHQLSSEEVDNLSPLL
                     GVAIGLALRSATR"
     gene            complement(4297..5370)
                     /locus_tag="PKH07_01280"
     CDS             complement(4297..5370)
                     /locus_tag="PKH07_01280"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNT33610.1"
                     /translation="MPDAKRLTEKGFALAKEGKFEKALRHFETALTQDPRYVEAIYGQ
                     GAVHYKLRDFRRAREFFSQVLNFDPTHERSIKYLHRCDDQLEQASYGTGTGSVSSWGS
                     SDVTLQWGGIQKDQSLSDASDAGGSQASNTGVSDWGVSSSVPEEESRLPVEPEPPQDS
                     NRQTQAPKRKPKAPIRNTRQMIWIFCVNAVLVVMLVSFLYGVAGLFRGEPQPKLLTPE
                     SSRDTEPESGESITLRIENDNVVGIVQPEPEESHPTEEKPSVQSDGSAVLPELLAILG
                     LVLALPPYIGFLVSAFQESFLKGVLFLWCCCYSPWYIFVEYKSESKWLHIGVYLVGNI
                     LCLVAHLLGSEIARYFLRRMTRF"
     gene            complement(5399..5821)
                     /locus_tag="PKH07_01285"
     CDS             complement(5399..5821)
                     /locus_tag="PKH07_01285"
                     /inference="COORDINATES: protein motif:HMM:NF022281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2752 domain-containing protein"
                     /protein_id="HNT33611.1"
                     /translation="MSEQSSRPLWRLSTFLEARVAWLCVLAIVASFFLPERGLGFSFC
                     LTRSLLGRPCPMCGLTRSVTCISHLHFEKAMHYHPLGFIIYVGLVVSSLVLLTPACKR
                     ERLRTWLRMSSLDWIPPTLFLSVLFVFGLWRFIWGVCP"
BASE COUNT         1402 a         1725 c         1411 g         1473 t
ORIGIN      
        1 cataccggaa gagacgaccg aagacgggct gagttcaggc tcaatagact ccagtccctc
       61 ttcggcctcc agttgctggt tgggctcttg cgcctgaacc tgcacctcgg attcatcccc
      121 gactgattcc gaaggcgttt gctcttgtgc ccaaaccatg ggcacagaca aaccgaccgt
      181 gcatgcggtc agcagtatca ggagcctcag aacgcccaga ggtatcttgc agcccgacca
      241 ttgtcttttg ttcaccctca tcgtcttcga ccctttcttt ttgtcttggc ttcaaattga
      301 ctactggttg tttcctgctc agacttttca aaatcctctg caggagaaac tcggaactct
      361 ttgttcatat acagaaccaa aacttcgtgc tgatggatat ccagcacttc aacgtcatca
      421 acgaagtccc caggacccgc caggcgccca ttgatcactg ccagacggcc tccctgatcg
      481 gtgctcatga taacctgcac tgacagcgga ggaagcctga ccggagtcgg cgtcggtgtc
      541 ggtgtgggac ccgccggagt cggcgtggcc ggcatcggag tcggcgtggg cgcttcggtc
      601 ggtacgatca ggggaacaaa cggatctttc agcccctccg gatcgtagag aaaccgactc
      661 tcactcgtgc cagcagcggc ttttggagca ggcgtctttt ctcccgtccc accaaccact
      721 ccgtaaacac gaacaaaaga aaaacccagc gcaaaaacga atatgagtaa ggatatccag
      781 cgccgcatcg ttcctcctgt tctcataaaa ccttctcccc ctgacagtaa tcctgtcgtt
      841 tttttctcat tccccgccct aactcttacc cgcacctgaa gcggcgggcg tttgctcgcc
      901 ttcctcgtct ttgttctcag attccgcaac gaaactgaat gtcgagaaaa ccaatccgac
      961 attgatacgc cccgggaatt cagggtcgcc ttggatgctc agatcatcca ctttggaaaa
     1021 acgctcggaa tactccgtca tattgatgaa attccccaaa gcgtgatagt ccgtcaccag
     1081 ctttatctcc aggggaagtt ccacatgaga actcttctcc cgaaacggtt gcatcttcat
     1141 gctcacatat ttctgccctg caacctgtgc caaggtgttc aactccgcca tcaaaccatc
     1201 caacctcggt gagtcaggca gtctgctctc ccacaattcg atcttgcgtt caaggtctcc
     1261 aacttcggca aggatgtctg ctttcttctt gacctgacgt tccttgtcct cgacttcttg
     1321 ctgtatcttt gcagcctgct ccgtgacctt cttgtactca ccgtataact ccttcagtcc
     1381 catgatcttc aatggcggct tctggaccgg tgcaggcttc ttggctcctg gctttttgac
     1441 tggagtgcca gattcatctc ccgcttctgc cgtctgcgcc tcgtctgtgg tctccagcgc
     1501 agtatcctga actgtctggg cgatcgtcac agcgggcttc ggagtgcttc caatctgaaa
     1561 tctgggaagg aagatcgaaa aaagaatcac tacaggaatg atcactccga caatttgaag
     1621 aactttcttg tctttttcag ttaaggccat tctactctcc cctgtactct aaccacgccc
     1681 acgggctggg cctatctctt cctctgctcc tccccgtgta ttgatgtcca tagaaatctt
     1741 gaaacgccac accagagggg aagtatcgtc attcagaacc caatgctctt ccctcgcttc
     1801 gacacctccc agattgctga agtcccgaga gaaactcgca ttcttgttga tattgctgat
     1861 aaactgaccc accaaggccg ccttgttggt cagatcatct gtgatcgctt cgattacaag
     1921 gcgtttttgg atcacgtagt aggtcttttg tcggaacttg ggcttgcctt gcgcatcctt
     1981 ggagccggga acctcttcct ttctcgtctt cttcacccga tcccctttga tgtacatgct
     2041 ctcaagccaa actccgtttg gaaccaacga ggccagttca aacagctttg gagcccagtt
     2101 taggcgctgt ccggccagaa tcttgatgac ccgttccttg ctggacaaca gagccttctt
     2161 gttctcgagc gccctgacct cttccagttc aacatccagt tcagcactgc gtagggtcag
     2221 agtctcaact cgctgctcct gattccgcaa ctgcatgttg gcgtagacgg ttcccataat
     2281 cacaagcccg accacaacgg cggttccggc aattgccaga tactgtgcca tcatgggcga
     2341 tcgggctttc cggcgctcgt gcggcaataa gttgactgta atcacggcgg aacagacctc
     2401 cccgcgtttc gcgctatctc gtggcgctgc gaagcgccag ccctatggct acacccaata
     2461 gagggctgag attgtccact tcttccgaac tcagttgatg attgatcttg tcaaggggtc
     2521 ggattatctc cgtctttacc ccaagcgtct cctcgaaaaa tgggcacaga ttgggcagca
     2581 tcgccgtccc acctgacaag atgatccggc cgattggctc gccgtcaagc tggttctcat
     2641 aaaacaagag cgaccgcttg acctcactgg tgatgtctcc cagaatctgc tcgcacactt
     2701 cagcgactcg accctggtct gccggacccg aatcagtcgc gcccatcacc ggctcatctc
     2761 ccaagcccag accctccaga gctttggtca catccgaaac gatatccggt tgggcgtact
     2821 ggttgccctc aatggtcaga gacaatgact cgctttcacc ttttcgagaa cccaaaccgg
     2881 tttcggcctt gatacgttct gcttcttcac gggtcttacc caaataggac atcaaagcgt
     2941 tggtcagatt cctgcccgcg atcgccaagt cgcgcgtgaa gcgggtcgca ttgtctttca
     3001 cgatggcaat gctggtaatc tcggcgccaa tgttcaccaa agccgccgat tccccggaaa
     3061 tctgattgct gcactccgcg taggcattca ccagcgcaaa ggcccctaca tctacgatac
     3121 agggctccaa ctgggcttct cgaagcagat gaacatggct gtccaccaaa tccttcttga
     3181 ctgcaacgat taggacttcc tgtacctgac cttgctctcc actccgttcc gagataccga
     3241 gcagttgaaa gtcgaaggca acgtcggcca tgtcaaaagg tatgaaatca cgcgcttcag
     3301 tctgaataga caattgaagc atctgttcat cctgattcgg gataaacgga atggtgatga
     3361 ttcgaacgat gaccgattct ccagagaccg ccgtcactac tcgccgattc tggataccat
     3421 gcgtcgaata gatatccttc agtgcggcca gtatcgcttc ccgactacga gccagagcct
     3481 tctcttccgc ggcttcgtct cggagagggt gaacgaccca acgcttcagc tcgtattggt
     3541 ctcccgtctt ttccagttcg accagtttca cagagtggct gccaatgtcc aaacccagtg
     3601 ctggccgaga acgaccgaag aggcgttcca ttcctgagag catcctcact cgtggcactt
     3661 ctttattaac cccttttctc tgttttctgg tcttactaac cttcagtgat cattaaagat
     3721 aatcacgatg cccttattat tagcttcagt tttcaattgt caagcaaaaa aaattcaact
     3781 ggtcgttttg gctttccaga ccttcttctt ggtattttcc ggttgtcaac atgcagtcaa
     3841 ctccaccttt cagtttcagc cgaaacgaga tgcggagaag atcggttgat ctcaccaggt
     3901 ctcttgctgt taatcctctt ctgctgcact ctgccatatt cgacatccgt gtcagcagat
     3961 ggcacaactc gtgccaattt gagcgaatga agatcctgaa ctgcataaag gccatgagac
     4021 aatcgcgctg atacctactc aatttattta ttttatttga cttaaatatc aacgactgaa
     4081 tcacgatggt attatctcaa acgttagtcc atggagttca ataggaaagg catacttgtg
     4141 caatatgtgt tccacagacc tgtatgactg ggaaaacttt tcctagtttc ctgtccctct
     4201 cgttgtccac agaaccagtt cccagaaatc cgactccggc cagaccgttt ctttcttaaa
     4261 tgagtctctt cggggagtga ccatagcctg caccgctcag aaacgtgtca tacgacgcag
     4321 gaagtaccgt gctatctcag atccgagtag atgcgccacc agacagagaa tgtttcccac
     4381 gaggtagacg ccaatgtgaa gccacttgct ctctgacttg tactcgacga atatgtacca
     4441 gggactgtag cagcagcacc agaggaaaag aacgcctttc agaaaactct cctgaaatgc
     4501 agataccaga aagccgatgt aaggcggaag agccagaaca agaccgagga tagccaagag
     4561 ttccggtagc actgcagaac catcagactg gactgagggc ttctcttccg ttgggtgact
     4621 ctcctcaggt tccggctgaa caatgccaac aacgttgtcg ttctcaatgc gcagggtgat
     4681 cgattctccc gactcgggct ctgtatcacg agaggactcg ggcgttagca gtttcggctg
     4741 aggctccccg cgaaacagcc ccgcaacgcc atagagaaag gagacgagca tcaccaccaa
     4801 aacagcattg acgcagaaga tccagatcat ctgacgcgta ttgcggatag gggctttggg
     4861 cttccgcttt ggtgcctgcg tctgtctgtt ggagtcttgg ggaggttccg gttcaaccgg
     4921 cagccgggac tcctcctcag gaacgctcga agacactccc cagtcgctga cgccagtgtt
     4981 ggaggcttga gaaccgccgg catctgatgc gtcagagagg gactgatcct tctgaatgcc
     5041 cccccactga agcgtgacgt cagaactgcc ccacgatgag accgaaccag ttccagtccc
     5101 ataggaagcc tgctccagct ggtcgtcaca tcggtgaagg tacttgatgg agcgttcgtg
     5161 cgtcgggtcg aaattcagaa cctgtgagaa gaactctcgt gctcttctga aatcccgcaa
     5221 cttgtagtga actgccccct gcccataaat ggcttccaca taacgagggt cttgggtcag
     5281 tgcggtttca aagtgccgta gcgctttttc gaacttaccc tctttggcaa gcgcgaaacc
     5341 tttttccgtg agtctttttg catccggcac gatggttcac cttctcgcaa aactgatgtc
     5401 aggggcagac tccccaaatg aaccgccaca ggccaaagac aaacaaaacg gacagaaaca
     5461 gggttggagg aatccagtcc agggaactca tcctgagcca agttcgaagc ctctcccgct
     5521 tgcatgcagg ggtcaacaga acaagactgg agacaaccaa cccgacgtat atgatgaagc
     5581 caagaggatg atagtgcatg gctttctcaa aatgcagatg tgaaatgcat gtcacactgc
     5641 gcgtcagccc acacatggga cagggacgcc ccaagagtga acgggtcaga cagaaactga
     5701 atcccagtcc tcgttcggga aggaagaaag aagccacaat cgccagaacg caaagccacg
     5761 caacacgtgc ttcgagaaaa gtggacaaac gccacagagg ccgtgaagac tgttcagaca
     5821 tgatgcgtgg aatgttacac gaatacgatg aaaaacaaaa gtgtgcatgc atctctgcta
     5881 tcacaattgt agtatggcca ttttgctagc cgcagatcgt gtgattccct cttgctttcc
     5941 tgttggggaa ctctatactt gcgcgagtat ttcattctgt accaaggagt ctgttttgat
     6001 gaaaacgcgt a
//
LOCUS       DAOQUC010000041         5937 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_14775_length_5937_cov_2.954437, whole genome shotgun sequence.
ACCESSION   DAOQUC010000041 DAOQUC010000000
VERSION     DAOQUC010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5937
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14775_length_5937_cov_2.954437"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..891
                     /locus_tag="PKH07_01290"
     CDS             <1..891
                     /locus_tag="PKH07_01290"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNT33612.1"
                     /translation="REGGQANVESKRGCPCGCVYCADPISKGRKQRLREPAAVITETR
                     NLLKQGCDCFHFCDCEFNVPKDHAEQLCHAIVSEGLQERISWYCYAIPLGMDQELISL
                     MKRAGCRGINFGVDHGVNSMLERLGRDFRQADIRRVIQCCKQEGVTAMLDLLLGSPGE
                     TAETVKEALRFVRNLEPDCVGVSAGVRLYAGTPLVNRLQQDGLLENNPNILGAASNNP
                     GLLRPVFYLEQGIRESLGSIMEDILGDDPRYFIGSNEPVDANYNYNDNALLVQAIQEG
                     ARGAYWDILRQIRTGSGSNL"
     gene            888..1511
                     /locus_tag="PKH07_01295"
     CDS             888..1511
                     /locus_tag="PKH07_01295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33613.1"
                     /translation="MKNKQLLRSFLLVLCLQMTIALMAHADVRVTLRSGATLSGRLVE
                     RKKEAIILRTQGSIKTIPMTDVRSVDLIAAPTGDILAPFIGSPPTALSPRSAQLLMDV
                     QGYADPTPKPETASGMLLPATPESPTADELAALLASPEIVEATLENDLPEPVTDERDL
                     IHLNSGGVLKGYLCEETDEHIVVLLPYGRIKIPRDQVQRVELVGTTQ"
     gene            complement(1456..5352)
                     /gene="addA"
                     /locus_tag="PKH07_01300"
     CDS             complement(1456..5352)
                     /gene="addA"
                     /locus_tag="PKH07_01300"
                     /inference="COORDINATES: protein motif:HMM:TIGR02785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HNT33614.1"
                     /translation="MTQDTKWTEAQRRAIQTVGKTLLVSAGAGAGKTAVLTQRCLYLV
                     ADRKPPTELNRILVVTFTEAAAAEMRQRIGDALRERLESDESNAYLRRQLALLEQSPI
                     STLHSFCLRLLREFFYELHLDPNCRVIEEDELLLLKEEIVTEVMEQFYAAEDEQGDHF
                     RSLVDCYGGVSGDNQLRQALLNLHSFLQGVPDSESWLEETLRLYQGIGSRSTLDGLPW
                     FSEFDRLFREELDALEQQARKVCSRQDIQSVPKYLRDATNLHKDFQAWCEFLPGNGVL
                     ETAKLIRSFKIPRKPSVPNAPEAASRALDALRERFKKGWLFSVSPYSMEDWLAGIART
                     QPHAQTLCEVYQAFRAALADAKKRAGCIDFSDMEHLCLRLLSDTSSVPGERHPSPVAL
                     SLRERFDHILVDEYQDINPVQDEILRLLSRESCRDSESNLFMVGDVKQSIYGFRLADP
                     GLFVQKHRTFSDTDEGHQMKVFLRENFRSRENIINGVNALFRLLMTESLSDIRYDQSQ
                     ELVCAAQYPDDDGLSAKSHPIEIHLLERKPLHRQEEDTPDGEPDEQTGKEENLEQADK
                     EACVVAELIQEMIEPVEGTPLQVWDNRKKCYRGASYRDVVVLLRALKEKAERFADVLR
                     RAGLPVYAEAGTGYLRATEIQDMLALLGVLDNPCQDIALSAALRSPIFRLQAGDLLRI
                     RRIKEDGDFYSAVKHYAQQQSDSLAERLREILEQIDSWRTLARQRPLSSVIWELFQQT
                     EYLSYVAGLPSGALRRANLLKLHNRSMQFDRFVRQGLARFLQFIERMEEVGADYGEAP
                     YLSEAEDVVRVMSIHKSKGLEFPIVIVPDLGKAFNFSDERGDILFDRNLFMGLKAVEL
                     ERMIKYPTLSHEIIGMRKHERTLAEEMRVLYVALTRAREKLILVGSVELEKTLGVWEQ
                     YRGEEDKGKLADALEDRFRRATRMTDWVGPAITVLSENSFPPAQESRTEVTMHCDIER
                     SAFFRVFLYPSAVVEQMSVQGRMSRSPLISEDCIESLSPYQIEQSSDSVVAKNILQQV
                     QWHYSHESQLQIPSKMSISEIKRRFDLQNNGSEEEETSHLQPVRLSRKPQFVEQSREE
                     ISSMERGLITHLVLQHLDLSIPLNEGAIRSQVKRMVEGELLTQEQAAEVLVKSLVQFF
                     ESDLGQRLQHSHNSVRREVPFSFAVPACSVIQDLPEQASSESILIQGIIDCLLEESDG
                     FTLVDFKTDRVTEEQVPDRALKYQLQMAVYRRAVRQAFGKPVKETILYFLRPRVAHSV
                     QANTESLDDSKLLLENLVSTAWYPPVQHAEPGREES"
     gene            5508..>5937
                     /locus_tag="PKH07_01305"
     CDS             5508..>5937
                     /locus_tag="PKH07_01305"
                     /inference="COORDINATES: protein motif:HMM:NF027716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HNT33615.1"
                     /translation="MTVQDDRGIENKVTQGHSERIHSIDVLRGLTILAMIFVNDIGEV
                     ASVPWWLKHFPQDKDGMTFVDVIFPAFLFMVGMVIPAALKRRKDKGDSMSAIWRHILT
                     RTLGLLIIGVFMVNTYASSIDGALSLPLWSLIGYVAVILIW"
BASE COUNT         1411 a         1632 c         1525 g         1369 t
ORIGIN      
        1 cgtgagggtg ggcaggcaaa tgtcgaatcc aagcgaggct gcccctgtgg ctgtgtctac
       61 tgtgccgatc ctatcagcaa aggcaggaaa caaagacttc gcgagcctgc agcagtaatc
      121 actgagaccc gcaacttgct caagcaaggt tgcgactgct tccacttctg cgactgtgaa
      181 ttcaatgtac ccaaagatca cgctgagcag ttgtgtcacg ccattgtcag cgaaggtttg
      241 caggagcgca tctcctggta ctgctatgcg atccccttgg gtatggatca ggaactgatt
      301 tccttgatga aacgggcagg atgtcgggga atcaactttg gtgttgacca cggagtcaac
      361 agcatgcttg aacggctggg acgcgacttc cgtcaggctg atatccgccg cgtgatccaa
      421 tgctgcaaac aggagggggt caccgcgatg ctggatctac tgcttggcag ccccggggaa
      481 acagcggaga cagtgaagga agcccttcgc tttgtccgga atcttgagcc ggactgcgtg
      541 ggcgtcagcg caggtgtccg actgtacgca ggcactcctt tggtcaaccg tctgcaacaa
      601 gacggtctgc ttgagaacaa cccgaacatc cttggtgctg ccagtaacaa tcctggcttg
      661 cttcgtcctg tgttttatct ggagcaaggg attcgcgaga gcctcgggag cattatggag
      721 gatatcctcg gtgatgatcc gaggtatttc attggaagca atgaaccggt tgacgccaac
      781 tacaattaca atgacaatgc tttgttggtg caggcaatcc aagaaggtgc gcgcggtgcc
      841 tactgggata tcttgcggca gattcgaaca gggagtggtt caaacttatg aagaacaaac
      901 aactcctcag aagcttcttg cttgtgttat gtctccagat gacgatagca ctcatggctc
      961 acgccgatgt acgagtgacc cttcgcagcg gagccacttt gagcggcaga ctggtcgagc
     1021 gcaagaaaga ggcaatcatc cttcgaactc agggcagcat caaaaccata ccaatgaccg
     1081 atgttcgatc ggtggatttg atcgccgcgc cgaccggtga catcctggct ccgttcattg
     1141 gaagccctcc cacagctctc tctccgcgaa gtgctcagct gcttatggac gttcagggat
     1201 atgctgatcc aacccccaaa cctgagaccg cttcaggaat gctcttgcca gcaacgccgg
     1261 agtcaccaac agccgatgaa ctggcggcac tgctcgcctc accggagatc gttgaggcta
     1321 cgctcgaaaa cgatcttccc gaaccggtca ccgatgaaag ggatctgatc catctcaaca
     1381 gcggaggcgt tctcaaaggc tatctctgcg aggagacgga tgagcatatc gtcgttcttc
     1441 tgccctacgg cagaatcaag attcctcgcg accaggttca gcgtgttgaa ctggtgggta
     1501 ccacgcagta gagactagat tctcaagaag aagtttggaa tcatcaagac tctcagtatt
     1561 tgcttgaaca gagtgcgcca ccctcggtcg aagaaagtag agaatggtct ccttcactgg
     1621 ctttccaaac gcttgacgaa ctgcccgacg gtagacagcc atctgcagtt ggtacttcag
     1681 agccctgtcg ggtacttgtt cttcagtgac tcgatccgtc ttgaaatcca ccagcgtaaa
     1741 gccatccgat tcctcaagca gacagtcaat gattccctga atgagaatgg actctgaaga
     1801 tgcctgttca gggagatcct ggatcaccga acacgctgga actgcgaaac tgaaaggaac
     1861 ctctcggcgc acactgttgt gactgtgctg aagtctctgt cccaagtccg attcaaaaaa
     1921 ctgaaccagc gacttgacca acacctcggc ggcctgctct tgcgtcagca attccccctc
     1981 gaccatccgc ttcacttgcg agcggatcgc accctcattc aatggaatgg aaagatcgag
     2041 atgctgcaag acaaggtggg tgatgagtcc tctctccatg ctgctgattt cctctcgcga
     2101 ctgctcgaca aactgaggct ttcgagacag tcggacaggc tgcaggtgag aggtctcttc
     2161 ttcttcgctg ccattgtttt gaagatcaaa gcgacgtttg atctcactga tggacatctt
     2221 ggacggaatc tgcaactgag actcgtggga atagtgccac tgaacttgtt gcaggatatt
     2281 ctttgccacc acggaatcgg aagactgttc gatctggtag ggcgacaaag actcaatgca
     2341 gtcttcagaa atcaacgggc ttcggctcat tcttccctgg acagacatct gttcaacgac
     2401 tgccgatgga taaagaaaga ctcgaaagaa tgcggatctc tcaatgtcac aatgcatagt
     2461 gacttcagtt cgactctcct gggcgggcgg aaaggagttc tcggacaaga ccgtgattgc
     2521 cggacccacc caatcggtca tccgggtcgc tcttcggaaa cgatcttcca aagcatcggc
     2581 gagtttccct ttgtcttctt cgcccctgta ttgctcccag acgcccagag tcttttccaa
     2641 ttcaacggaa ccgacgagga tcaacttttc tctggcacgg gtcagcgcga cgtaaagaac
     2701 cctcatttcc tccgcgagtg ttcgttcatg cttgcgcatc ccgatgatct catgagagag
     2761 cgtcgggtac ttgatcatgc gctccagttc cacagccttc agccccatga acaggtttcg
     2821 gtcaaaaagg atgtcacctc tctcgtcaga gaagttgaag gccttcccca gatccggaac
     2881 gatgacaatt gggaattcca atcccttgct cttatggatg gacatcacac gcacaacgtc
     2941 ttccgcctct gaaagatagg gtgcctcgcc atagtcggct ccaacttctt ccatgcgttc
     3001 gatgaactga agaaatcgcg ccaagccctg tcggacaaaa cggtcgaact gcatacttct
     3061 gttgtgaagt ttcagaaggt ttgcacgtcg aagtgccccg ctgggaagtc cggcaacgta
     3121 ggagaggtac tccgtctgtt gaaacaactc ccaaatcacc gaagagagag gtcgctgtcg
     3181 agcgagtgtt cgccaggaat cgatctgctc caaaatttcg cgaagcctct cggcaaggga
     3241 atctgattgc tgttgcgcat agtgtttgac tgcggaatag aaatccccat cttccttgat
     3301 acgccgaatt cgaagaagat cgcctgcctg cagacgaaag atcggagacc gcagagccgc
     3361 cgacaaggcg atgtcctgac acggattgtc caagacaccc agcagtgcaa gcatgtcctg
     3421 aatctccgtc gcgcgaagat agcctgttcc cgcttccgca tagacgggaa gtcccgctcg
     3481 gcgaagaaca tcggcgaaac gttcggcctt ctcctttaat gcgcgcaaca atacgacaac
     3541 gtctcgatag gacgcgccgc gatagcactt ctttctattg tcccagacct gaagaggcgt
     3601 gccctcaaca ggctctatca tctcctgaat cagctctgcc acaacacagg cctctttgtc
     3661 tgcttgttcg agattctcct cctttcctgt ttgttcgtcc ggttcaccat cgggtgtgtc
     3721 ttcctcctgg cggtggagag gcttgcgctc cagaaggtga atctcgatag gatgagactt
     3781 cgccgacagg ccatcatcat caggatactg cgcagcgcag accagttcct gactctggtc
     3841 gtatcgtatg tccgaaagac tctctgtcat cagaagcctg aagagtgcgt tcacgccatt
     3901 gattatgttt tcacgagatc ggaaattctc ccgaaggaaa accttcatct ggtgcccttc
     3961 atccgtatcg gagaatgttc tgtgcttttg aacaaaaaga ccgggatctg ccaggcggaa
     4021 tccatagatg ctttgcttca cgtctccgac catgaacaga ttcgactcag aatccctgca
     4081 actctctctc gacagcaacc gcagtatctc atcctgaacc ggattgatgt cctggtattc
     4141 atccaccaag atgtggtcaa agcgttcccg aagactgagc gcaaccggag aggggtgtct
     4201 ttctccaggc acagacgaag tgtccgacag gagacgcaaa cagagatgct ccatatcaga
     4261 aaaatcaatg caacctgccc gtttcttggc atcagcaaga gcggctcgaa aggcctgata
     4321 cacttcgcaa agcgtctgcg cgtgaggttg cgtgcgtgcg atgccggcaa gccagtcttc
     4381 catggaatac ggggagacgg agaacaacca gcccttcttg aaacgctccc gcagagcatc
     4441 caacgcacgg cttgcggcct cgggcgcatt cggaacggac ggcttcctcg gaatcttaaa
     4501 tgacctgatc aacttcgccg tctcaagcac gccgttgcca ggcagaaact cgcaccaggc
     4561 ctgaaagtcc ttgtgaagat ttgtagcatc ccgaaggtat ttcggcaccg actgaatatc
     4621 ctgtcggcta cagactttcc tggcttgttg ctcaagagcg tcaagttcct ctctgaaaag
     4681 tcggtcgaac tccgaaaacc atggcaagcc gtcgagagta cttctcgagc cgattccctg
     4741 gtacaaacgc agtgtctcct caagccaact ctctgagtcg ggaaccccct gcaagaatga
     4801 gtggagattc aggagcgctt gtcgcagttg attgtcgcca ctgactcccc cgtaacagtc
     4861 caccaacgat ctgaagtgat ccccttgctc atcttctgcg gcatagaatt gctccatgac
     4921 ctcagtcacg atttcctcct tgagcagaag gagttcatct tcctcgatca cacggcagtt
     4981 tggatcgaga tgcaactcat agaagaattc acgcagcaga cgcaggcaga aggagtgcag
     5041 cgttgagatg ggagactgct ccaacagcgc cagttgtcgc cggagataag cattgctctc
     5101 gtcggactcc agtcgctccc gcagtgcatc gccaattctc tggcgcatct cagccgctgc
     5161 ggcttcggta aaagtcacga caaggattcg gttcagttca gtcggcggtt ttcgatccgc
     5221 gacaagatac agacagcgct gagtaagaac cgcagtcttg ccggctccgg cacctgcaga
     5281 caccaagaga gtcttgccaa cggtctgaat cgcccgccgc tgtgcctctg tccattttgt
     5341 atcctgtgtc atgcgactcc acccttccac ggagcctggc aatacatcgc tgtgaccctc
     5401 ccactattcg gcacaagtca aagtatttgt atgacatttg aaacaccatt gcaagcaagg
     5461 gagcaatcac gtaataatga ttcaaacaaa accggtgagg ttcacaggtg acagttcagg
     5521 acgacagagg catcgaaaac aaagtgaccc aagggcattc cgaaagaatc cattccatag
     5581 acgtccttcg aggactgacc attcttgcca tgattttcgt caatgatatc ggtgaagtgg
     5641 caagtgtccc ctggtggctc aagcattttc cgcaggacaa agacggcatg acctttgtgg
     5701 acgtgatctt tccggcgttc ttgttcatgg tcggcatggt catcccagcc gctcttaaga
     5761 ggcggaaaga caagggcgac tccatgtcgg cgatttggcg tcatattctg accaggacac
     5821 tggggcttct catcatcggt gtgttcatgg tcaataccta tgcgtcctca attgatggcg
     5881 ctctgtctct gcctctgtgg agtctgatcg ggtatgttgc ggtgatcttg atctgga
//
LOCUS       DAOQUC010000042         5889 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_14945_length_5889_cov_3.031368, whole genome shotgun sequence.
ACCESSION   DAOQUC010000042 DAOQUC010000000
VERSION     DAOQUC010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5889
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14945_length_5889_cov_3.031368"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1632)
                     /locus_tag="PKH07_01310"
     CDS             complement(<1..1632)
                     /locus_tag="PKH07_01310"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HNT33616.1"
                     /translation="MAAKELILFVPLDPVHDVGIRLIEQGLREQGYLTKLLPPDLPIE
                     DIVRVAAEEDYAFIMVGRTISYQTAELMARLVDMLDRAGVRQKAKLVAGGKAMTPELA
                     AELGFDKGFDANTKVEHVVAFVQGRPITADDDQVRRVKKDVTAPYSYQWRNSEAEQLC
                     FAVADQVLDWASRHTSPGVARAQLRKLILTCESDSERNLLRLEYASLCDPSIASAYRE
                     GIPVEGTRWIEPHEVDLLEQIPATRRLNPIRTSDAQPLVIFFTGSGCPIMDIQHNRIA
                     AECGADGTIVICPSWAARREGMLSELVTHQHDGTVPSVENVLLMKNYLREDLYFQLRA
                     HRGLNTAETSVIAVHAGVDFCKVNPIYGSLGGGTDPERLVLDAVYAIRTVAEAGTPYD
                     MPANDELCGIPFAKNLAGMLVTAAIGLRVGGRPIMKPLFCYSPNVMVGGQMKDNYVDY
                     NAAKIAALRGIIDAPIWPGEPVGFYTHEDDRCQSATTTALHAALAASLGVDLMTFAST
                     DESYSRGPITIASRVDTFNALRAMFRFFGSTKITPT"
     gene            complement(1634..1870)
                     /locus_tag="PKH07_01315"
     CDS             complement(1634..1870)
                     /locus_tag="PKH07_01315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33617.1"
                     /translation="MKQDVRCLARVVDAIDLETVFVCSSELEAKELSWELLSHLGLEG
                     DIVSLQFTGSSARVRLRGNVHLPGDTYSWGRRES"
     gene            complement(2146..3618)
                     /locus_tag="PKH07_01320"
     CDS             complement(2146..3618)
                     /locus_tag="PKH07_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012283754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33618.1"
                     /translation="MAQEQMKYRFRMYEKLQKEIPQIYAELKEAAKELKLKDDEKNAI
                     GLTGAISGCPGSLRDDILEAMHQKAKKVISNAALVDEIRDIVKSYYGDDYDACPVNTC
                     EAALWVSFDCLATPPFTGRGDNYRARYVAPFERHLHHQGGYGRPFPPKYKDVLADRGS
                     TAGELGFAGKRQNNLDTVFVPLPGAKYDVHGIRSAQVFMMKNVDADRSEKVLQEYAAR
                     HADSLTAVSSLGYDSTGYGYGDKKKNGAPKLQCALAKIAQKYNVPYIVDNAWGLPFVG
                     TDLRKTGADLMLYSMDKASGAPTSGLIIGKEDVMVPIRRALGVHGARWGTTSSYGKAA
                     YVTQDPGKEALSGQIAALKVLRDNPEVIVNPLEDLYRLVVEEFDKINPVLRPFFQIEK
                     SYNSSAIEINYESSWEGTGMGIPVFSIEDMYSGSHILQNGLAQIGIIPTIAYDANIFI
                     SAGLGTSDNKGQLIVEKMRKAIRALVLLLEITCKHVGLIG"
     gene            complement(3636..4094)
                     /locus_tag="PKH07_01325"
     CDS             complement(3636..4094)
                     /locus_tag="PKH07_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015758764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HNT33619.1"
                     /translation="MAIAQQLKNLGLDIPEAPVPVAAYVPAVRSGNLIYTAGQVPFVK
                     GQLAFSGKVGSELTVEQGAEAAKICVLNALGAVRSLIGDLERVVRIVKLTGFVNCGSD
                     FTEQAKVMNGASELLLALFGDKGKHARCALGANALPLNAAVELDLIVEVE"
     gene            complement(4098..4535)
                     /locus_tag="PKH07_01330"
     CDS             complement(4098..4535)
                     /locus_tag="PKH07_01330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33620.1"
                     /translation="MADPYKDKLKVSAIYSAKRSVAACFVAILDLRMEDRGLQLIVPQ
                     SRAVRQGEVIEIISTSEAGAKPGGTVQTASYFGFAEVQCSGMILVGDQVRLGGETIAR
                     VAGFDETHAPNHINVVLSMDPARTGAEMCLAIEQEIEFAFKEV"
     gene            complement(4637..4894)
                     /locus_tag="PKH07_01335"
     CDS             complement(4637..4894)
                     /locus_tag="PKH07_01335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33621.1"
                     /translation="MRAGSSLTQRNTPQTLLLVFEPSDITITGSNGLLTLNNRSMDGS
                     VSIPPYGEQANAYWRVGDALSWKGLLSMVTRLLDSLSVGVV"
     gene            complement(4912..>5889)
                     /locus_tag="PKH07_01340"
     CDS             complement(4912..>5889)
                     /locus_tag="PKH07_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019934191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HNT33622.1"
                     /translation="EESLPNVRAENHAAEGGNESDVQARPKSQKTSEDKDKSRFEDTL
                     LQELVVMPDSFFAGQSATEIALRTRYGINLLAISRQGRRSVKRLRSTMIRAGDVLLMQ
                     GTADALAVFASEFGCLPLVTRDIRVPIRGQAVYGAAIMFLAVLLAALGLVPVAISFAV
                     AVLAFMVLRIVPVRSVYHSIDWSVIVLLGALLPVAETMGHTGAADVIARFLLQSVAQG
                     QPEICLTLVLVLTMLMTDFMNNAATAAVMSPIALSVAGQLGVNPDGFLMAVAIGSSCA
                     FMTPIGHQNNTLILGPGGFRFGDYWRMGLPTDLLVIAVSLPMILRVWPL"
BASE COUNT         1403 a         1698 c         1482 g         1306 t
ORIGIN      
        1 cgtcggggtt atctttgtgg atccaaagaa ccgaaacatc gcccgaagag cattgaatgt
       61 atccacacgg gacgcgatgg tgatgggacc tcgggagtag gattcatccg tcgaagcaaa
      121 ggtcataagg tctacgccca gagaagccgc cagagccgca tgaagtgccg tcgtagtcgc
      181 cgactgacat cggtcatcct catgtgtgta gaaaccaacg ggctcaccag gccagatggg
      241 agcgtcaatg atccctcgta gcgcggcgat ctttgcggca ttgtaatcta catagttgtc
      301 cttcatctga ccgccaacca tgacattggg tgaatagcag aagagaggtt tcatgatcgg
      361 ccttcccccg acacgcaagc caatcgctgc cgttaccaac ataccggcaa gattctttgc
      421 aaagggaatg ccgcacagtt catcattggc cggcatgtca tagggagtgc ccgcctcggc
      481 gaccgtgcgt atcgcgtaga cggcatccag aacaagtcgc tccggatcag tgccaccgcc
      541 cagtgaacca tagatagggt tgaccttgca gaagtccact ccagcatgaa ctgcaatcac
      601 actggtctct gcggtgttca gtcctcgatg ggcacgaagt tggaagtaga gatcttcgcg
      661 aaggtagttc ttcatcaaga gaacattctc aacactgggc acggtgccat cgtgctgatg
      721 ggtgaccaat tcgctgagca tgccttcccg ccgagctgcc caggaagggc agatcactat
      781 cgtcccatcc gctccgcatt ccgcggcgat acggttgtgc tgaatgtcca taatcggaca
      841 gccgcttcca gtaaagaaga taaccaaagg ttgtgcatcg ctcgttctga tcgggttcag
      901 acgtcgcgtg gcgggtattt gctccaacaa gtccacttca tggggctcga tccatcgagt
      961 tccctcgacg gggatgcctt cacgataggc tgaagcgatg gacggatcgc aaagagacgc
     1021 atactcaagg cgcaggagat tcctttctga atcgctttca caggtcagaa tcagttttct
     1081 cagctgcgca cgggcgacgc ccggactggt gtgcctggaa gcccagtcga gaacctgatc
     1141 ggcaactgca aaacacaact gctcggcttc agagtttctc cattggtaac tgtagggggc
     1201 tgtcacgtcc ttcttgactc gtcggacttg atcgtcgtcc gcagtgatcg ggcgtccctg
     1261 tacgaaggct acgacgtgct ccaccttggt gttggcgtca aatcccttgt cgaagcccaa
     1321 ctctgcagcc agttccggcg tcatggcctt cccaccagcg accagtttgg ctttctggcg
     1381 gacaccggca cgatccaaca tgtctacaag tcttgccatc agctccgcgg tctgataact
     1441 gattgttctg ccaaccatga tgaaggcata gtcctcttca gccgccacgc gaacgatgtc
     1501 ttcaatggga agatcggggg gcagcagttt cgtcaggtag ccctgctcgc gcagaccctg
     1561 ctcgatgagt cgaatcccaa cgtcatgcac gggatccaga gggacgaaga gaatcagttc
     1621 tttcgccgcc atatcaggac tccctccttc cccaggagta ggtgtctcca gggagatgga
     1681 cattgccccg aagtctgact cgtgccgagg aacccgtgaa ttggaggctc acgatgtcgc
     1741 cttccagtcc caagtgactc aagagctccc aggacagttc cttggcttcc agttcgctgg
     1801 aacacacgaa aaccgtctca aggtcaatgg catccacaac gcgcgccaaa caacgaacat
     1861 cctgtttcat agatctcgcc tcgaatctgc ccttctgtat gaagaggcta cgtcaaacac
     1921 acccctgcgt cgtgtccttg agagcgtggt tccggcattg cggaaccaaa ggaagaatct
     1981 ctcaaggctc cacgggtttc tcgtatccca gagagattct tcgcttcact ttcgacgttg
     2041 cattcagtac agcattctgt gaactctggt tgattgcgac acagtctcga agtgaaaggt
     2101 ctttctgaag agcctctctt tcttcgcatt ctgcagcaca ggctgtcagc cgatcagccc
     2161 tacgtgcttg catgtgatct ccagcaagag aaccagagca cgaattgcct tgcgcatttt
     2221 ctccacaatc aactgaccct tgttatccga cgttcccaag ccagcggaga tgaatatgtt
     2281 ggcatcgtat gcaatcgttg ggataatccc aatctgtgca aggccattct ggagaatgtg
     2341 actgcccgaa tacatgtcct cgatgctgaa gacgggaatt cccataccgg tgccttccca
     2401 actggactca tagttgattt cgattgccga tgagttgtaa ctcttctcaa tttggaagaa
     2461 cggtcgcaac acggggttga ttttgtcaaa ttcctcgacc acaaggcgat aaagatcctc
     2521 aagaggattg acaatgactt ccggattgtc ccgaagcact ttcagagcgg caatctgtcc
     2581 cgacaaggct tccttgcctg gatcctgggt cacgtaggct gccttgccgt aggaagaggt
     2641 cgttccccag cgcgctccat gtacaccaag agcacgtcga atcggcacca tgacgtcctc
     2701 tttgccaata atcaggccgc tggttggagc gccactggct ttgtccatgc tgtaaagcat
     2761 caaatctgca ccggtcttgc ggagatcggt tccgacgaaa ggcagtcccc acgcattgtc
     2821 cacaatgtaa gggacattgt acttctgagc gatctttgcg agagcgcatt gaagtttcgg
     2881 tgcgccgttc tttttcttgt ccccgtagcc gtagccggtt gaatcatatc cgagagacga
     2941 gaccgcggtc agggagtcgg catgtcgagc tgcgtactcc tgcaggactt tctccgagcg
     3001 gtctgcatcc acgttcttca tcatgaaaac ctgagcgctt cggatgccat gaacatcgta
     3061 ctttgctcca ggcaggggca cgaagaccgt atcaagatta ttctgacgct tgccggcaaa
     3121 gcccagttct ccagcggtgg agccacggtc tgccaagaca tccttgtatt tcggcggaaa
     3181 cggtcgtcca tagcccccct gatgatgcag gtgtctctcg aagggggcaa cgtaacgcgc
     3241 tcggtagttg tctccgcgac cggtgaaagg aggtgtggca agacagtcaa acgagaccca
     3301 gagcgcggct tcacaggtgt tgaccggaca ggcatcgtaa tcgtcaccat agtagctctt
     3361 cacgatgtca cggatttcat ccaccagcgc ggcgttcgag atgaccttct tcgccttctg
     3421 gtgcatggct tccagaatgt cgtcccgcag agagccagga catcccgaaa tagcaccggt
     3481 caaaccgatg gcgttcttct catcatcctt aagtttcagt tccttggcgg cttccttcaa
     3541 ctccgcataa atctgaggga tctccttctg gagtttctca tacatccgaa aacgatactt
     3601 catctgctcc tgagccatta tatcctcctt aacggttact caacttcaac gatcagatcc
     3661 aattccaccg ccgcattcaa gggcagcgcg tttgccccaa gcgcacaccg cgcgtgcttg
     3721 cctttgtcac caaagagcgc aagcaagagt tcagatgctc cgttcatgac cttggcctgc
     3781 tcggtgaaat ccgaaccgca gttcacaaat ccagtcagtt tcacaattct aacgacacgc
     3841 tccagatcac caatcaatga tcgtactgca cccagagcat tcaagacgca tatctttgcc
     3901 gcttcagctc cttgctcgac agtcagttcc gatccgacct tgccgctgaa cgccaactgc
     3961 cccttcacaa agggcacctg tcccgctgtg taaatgagat tccccgatcg aactgctgga
     4021 acatacgcgg cgacaggcac aggcgcttcg ggaatgtcca gccccagatt cttcaattgc
     4081 tgcgcaatag ccaccgttca aacctccttg aaagcaaact cgatttcctg ctcgatcgcc
     4141 agacacatct cagctcccgt tctggcggga tccatactca gaacaacgtt gatatgattg
     4201 ggtgcgtgtg tttcatcgaa tccagcaact cgcgcaatgg tctctccacc caggcgaacc
     4261 tgatccccga ccagaatcat ccccgaacac tgcacctccg caaaaccaaa gtaggatgca
     4321 gtttgtactg ttccgcctgg cttagcaccg gcttcactgg ttgaaataat ctcgatgact
     4381 tccccctgtc tcaccgcccg agactgggga acgatcagtt gcagacctcg gtcttccatt
     4441 cgaagatcca aaatggcaac aaaacatgct gcaaccgatc tctttgccga gtagattgca
     4501 gaaaccttca gcttgtcctt gtaaggatca gccattgacg ccctcctggc agtcattcct
     4561 ttgagaccgg aaaccaaaca gactttatga atatagcgca gacggaaacc ctctgtcaaa
     4621 gcgggctaag tctgattcaa actactccta ccgacagaga gtccagaaga cgtgtcacca
     4681 tgctgagcaa gcctttccat gagagggcat cgcccacgcg ccaataggcg ttggcctgct
     4741 cgccgtatgg cggaatggat accgacccat ccatggatcg attattcagc gtgagaaggc
     4801 cattgctgcc agtgatcgtg atatccgaag gttcgaacac aagcagaagt gtttgtggtg
     4861 tgttcctctg agtgagagaa gacccggctc gcaactccaa agccccgcag actacaacgg
     4921 ccagactcga agaatcatgg ggagactcac agcaatgacg agcaaatctg tcggcagacc
     4981 catgcgccag tagtcgccaa aacgaaagcc tccgggacct aagataagag tgttgttctg
     5041 gtgtccaatg ggagtcataa aggcgcatga ggatccaata gcgaccgcca tcaggaatcc
     5101 atccggattc actccgagtt gccctgcaac cgatagggcg attggactca tcactgccgc
     5161 cgtggcagcg ttgttcataa agtcggtcat gagcatggtt aagacaagaa ccagagtcag
     5221 gcagatttcc ggttgtccct gtgcaacact ttgaagcaga aagcgggcga tcacgtccgc
     5281 cgccccagtg tgccccatcg tttcggcgac aggaagcaac gctcccagca gtacaataac
     5341 cgaccagtca atagaatggt aaacagaacg aacaggaacg atccgcaata ccataaaggc
     5401 gagcacagca accgcaaacg aaatggctac cggaaccaag cccagagccg ccagcaggac
     5461 agccaggaac ataattgcag cgccgtagac cgcctgtccc ctgatcggca cccgaatgtc
     5521 gcgggtaacc aagggaagac atccaaactc agaggcgaaa acagccagag catcggcagt
     5581 tccctgcatc agcaagacat cgccagcccg tatcatggtt gaacgcaagc gtttgactga
     5641 tcgcctgccc tgccgtgaaa tggcaagcag gttgatacca tatcgagttc gaagggcaat
     5701 ctctgtcgca gattgtccag caaagaatga atccggcatc accaccagtt cctgaagaag
     5761 ggtgtcttca aatcgagatt tatctttgtc ctcggaagtc ttctgtgact ttggcctggc
     5821 ttggacatca ctctcgttgc caccttcagc cgcgtggttc tctgcccgga cgttcggaag
     5881 gctctcctc
//
LOCUS       DAOQUC010000043         5860 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_15036_length_5860_cov_3.544358, whole genome shotgun sequence.
ACCESSION   DAOQUC010000043 DAOQUC010000000
VERSION     DAOQUC010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5860
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15036_length_5860_cov_3.544358"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2065)
                     /locus_tag="PKH07_01345"
     CDS             complement(<1..2065)
                     /locus_tag="PKH07_01345"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HNT33623.1"
                     /translation="MVWSKPVFVSLLFLCLLALSTDTPAEISIPKVLPFAASDEVRIV
                     TTLQTDAAVDFLDLHATISSCRDSSVLWDGYLGRAIISGATTTDFSSRVTHLKPDLWS
                     PVSPLLYRLHLKAVRGQKTLAETTIRFGFRSFENREGRFFLNGQPIFLRGVAINPPGR
                     GVPDNVGFSKEFAEAYIRFLKSHNVNLIRLHKNSQDWFDLCDELGMMVFQGCYGSPPP
                     GSKTRLPTDIEKTIHLYKRRYFEDYVRHPSIIIYILSNELPYYGQLAKPYEEILQQVH
                     EHLREWDTNRLYIGNAGYGNGTSGDIFDFHRYWGWYYGSFLTYHVLRDYEPYKVLRKN
                     PDDNPQPMTMTECVGCYNMPNGCLNLVHRQRASGLGWTGYTQNKPDADLEYHALVLKN
                     AVELFRRLRKYNPKLSGIMPFTILFNRWSDIRCFDDMVPKPAMKQMQVSYQPVLLSWE
                     LWTPNVYSGSTVRPIAHVVNDSDDFQDLPASSLEYCIVTQDGARILTQKVNVPAVKYS
                     ECRSVPVSIRLPRSLPTGEYTIQGHILVDGQVRSLNDTQIFVEQIANEKKPPISPTRS
                     VALFDPQATTRDALKSLGFSVSVVDDIGKVADQGTLIVGEDAFDSDVGAATETVRRLI
                     QNGGRVLFLKHQDWRTVAQCIPLKIRALSASPIDVAYPQRQRPYVLGSFINPLRPEHP
                     CFAGIT"
     gene            complement(2444..3937)
                     /locus_tag="PKH07_01350"
     CDS             complement(2444..3937)
                     /locus_tag="PKH07_01350"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNT33624.1"
                     /translation="MANTDLNVLWITMDELHREALSCCGATSHKTPNVDRIAGEGMRF
                     ENAYAASPVCLPSRVSLATGLYPHRSRSFSNHTGASLSLSLPNVFQVFRSQGYQTSMH
                     GKCHFIPVPYPYVSRDFTREYDHFRQYYLDLGIDHLDLQDGNEVSAWFYDDYGKDLEK
                     AGLMSQYRDLVHNKTGSRCDKNLFLFPGDESMHPDSWVGRKAIEYLSHLDNNQPHFMW
                     VSFSGPHYPLNTPVEYLSRVDMSKDRAANKRDGEWDDPTKSNCVSYHGPGGTEGSNSA
                     EGGAQKNFDDSYWRQWRQMYYANVVQIDDYIGQILERAKALWGENLLVVFTCDHGDMM
                     GHHGLWGKNGAFFEDIIRVPLFIRPPRNRGGSVHQELVSLVDVLPTCLDAAGIDPIQC
                     DGKPLGELVAAGGRDYVLSETQNLVSIVHGSDKLTRHQRGETTFLEQYDLSRDPFEFD
                     NVSGEPSGLHARQQMESILERLQREENLHSILFYDPTDQRRPPWLRL"
     gene            complement(3967..>5860)
                     /locus_tag="PKH07_01355"
     CDS             complement(3967..>5860)
                     /locus_tag="PKH07_01355"
                     /inference="COORDINATES: protein motif:HMM:NF025665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 99-like
                     domain-containing protein"
                     /protein_id="HNT33625.1"
                     /translation="WEIEAEDAFRRELRLECFVSANRVEEAVLSINFLSAVERRSLSY
                     IPEPNPVKTSVVVGAHHCPLWEADKPFMWSQLLKHPERTPALGFYAQENPEVSDWETK
                     WAVEHGVSFFLYCWYRASQGEPVKMRFGSAIHEALFKSRFVNHMKFCIMWENQSRGTS
                     GVADLDDLTTNVLPFWMENYFKHPSYLKMDGKPLLFIYRPEFLIQDLGSEENVLEAFD
                     LMRQACQDAGFDGLYLLGEYRGLDPKHLELMKRIGLDYTFAYCWYVGNNPTPEQAIAA
                     QVDYINKTQALGILPQVVTVSQAWSGWADEGTIWKIPPTEFKTLLAKAKEIIATFPAN
                     ELGSRMLLLDNWNEWGEGHYIAPYREYGFGYLDAVREVFSDAPTAHEDLIPEDIGLGD
                     YDRPYRTHQETEKALRRQVSRKAFKGSETEEGLVAWWAFDEEEDSPVALDYSGHRLGG
                     IVRDAQYAPGIAGNALVCDGGCVEVPSSEMLNITSTLSVLCWVKTDRPGQNNTWFLNR
                     VLQGGTHTGFRMGVLDGKPSFHIPMTSWSHHLNSDTPLPIGRWVHLAGTFDGTTMRLY
                     MDGVLCGTMERPGTINTNDFHLILGNYDVKHAAHFVGLLDEVKLYRRALSPEEIRSAW
                     QALAEP"
BASE COUNT         1352 a         1563 c         1547 g         1398 t
ORIGIN      
        1 gtgtaatgcc agcaaagcaa ggatgctcag gccgaagcgg gttgatgaac gaaccaagaa
       61 cataaggtct ctggcgttgc ggataggcca catcaattgg agaagccgac agtgcgcgga
      121 ttttgagggg tatacattgg gcaacagtgc gccagtcctg atgcttcaga aagagcaccc
      181 gaccgccatt ctgaatcaat cgcctgacgg tttcggtcgc agcacccaca tctgagtcga
      241 aggcgtcctc accgacgatc agtgttccct ggtcagcaac cttcccaata tcatccacca
      301 cggaaacact gaagccgaga gacttaaggg catcccgtgt cgttgcctgc gggtcaaaaa
      361 gagcaacaga acgcgtcggc gaaatcggcg gcttcttctc gttggcgatc tgctcaacga
      421 atatctgcgt gtcgttgaga gaacgcacct ggccatctac gagaatgtgt ccctgaatcg
      481 tgtactcacc tgtcggcaat gatctcggca gtctgatgga caccggaacg cttcggcact
      541 ccgagtactt tactgcgggt acgttcacct tttgggtgag tattctggct ccgtcctgtg
      601 tgacgatgca atactcgagc gaagaggcag gcaagtcctg gaaatcatcc gagtcgttca
      661 ccacatgtgc gatgggtcgg acagtcgagc cggagtagac gttcggagtc cacaattccc
      721 aactaagcag caccggctga taggaaacct gcatctgttt catggctggc ttcggaacca
      781 tgtcatcgaa acaccggata tctgaccacc gattgaaaag gatcgtgaag ggcatgatgc
      841 cggacaactt ggggttgtac ttccgaagtc ggcggaacag ttcgacggca tttttgagca
      901 ccagggcgtg atactccaag tctgcgtcag gcttgttctg tgtgtacccc gtccatccga
      961 gtcctgatgc gcgttgccga tgaaccagat tgagacaacc gttcggcata ttgtagcagc
     1021 cgacacattc agtcatcgtc atgggttgcg gattgtcgtc gggatttttc cggagcactt
     1081 tgtacggttc gtagtccctc aggacgtggt aggtgagaaa agagccatag taccaccccc
     1141 agtatcggtg gaaatcaaaa atatcccctg aggtgccgtt gccgtaaccg gcgttgccga
     1201 tgtagagccg attggtatcc cattcgcgca agtgttcatg cacctgctgc aagatctcct
     1261 cgtaaggttt ggcaagttgc ccgtagtagg gcagttcatt ggaaagaatg tagatgatga
     1321 tggaagggtg tctgacatag tcctcaaagt atcggcgctt gtaaaggtgg attgtcttct
     1381 caatatcggt tgggagtcgg gtcttcgacc cgggtggcgg tgatccgtag caaccctgaa
     1441 ataccatcat gccgagttca tcgcagaggt cgaaccagtc ctgggaattc ttgtgaagtc
     1501 tgatgaggtt tacgttatgg cttttgagaa aacggatgta ggcttccgcg aactccttgg
     1561 agaagcccac gttatcggga actcctcgac cgggagggtt gatcgcaaca cccctcaaaa
     1621 agatcggttg gccattcagg aagaaccgtc cctctcggtt ttcgaaagat cgaaatccga
     1681 aacggatggt tgtttctgca agagtcttct ggccgcgtac cgccttgaga tgaagccgat
     1741 agaggagcgg ggaaacagga ctccacaagt ccggtttgag atgggtcaca cgggaagaga
     1801 aatcggtcgt ggtcgcaccg gagataatcg cccgtccgag atatccgtcc cagagtacgg
     1861 agctatcacg acacgaagag atcgttgcgt gcagatccag gaagtccact gcagcatcgg
     1921 tctgaagtgt cgttacgatg cgcacttcat ccgaagcggc aaagggaagg acttttggga
     1981 tcgagatttc cgctggagtg tctgttgaaa gagccagcag gcacaagaac aagagagaca
     2041 caaagacagg tttgctccat accattctca cgctcctgat agaaaaaacg ccagatcggc
     2101 gctggaatca tgcccaccct tgcgtctgcc tgtccagaga tagaaaccgg attaagaact
     2161 tttggaagaa cgtattactt gttctttttc ttttcctgct tggctttgcg tttttctttg
     2221 agactcagca ctgctttctt ctgctcttct cgtttgcctt tgtctttttt acccttgtca
     2281 gccatttgtt gctctcctta ggatcggact gagccgatag cggtggaatg actttgctca
     2341 tcccatgttg cccacctgtg tggtgctttt ctctctcttt ataccgcgtt atgccgctca
     2401 aggcaagcac ggatcacact tccgcgcacg ttgtgcgttc tcttcacaac ctcagccatg
     2461 gcggacgcct ttggtctgtg ggatcgtaga agaggatcga gtgaagattc tcttcccgtt
     2521 gcagtctttc gagaatgctc tccatctgct gacgtgcgtg aagtccagac ggctcaccag
     2581 agacattgtc aaattcaaag ggatcgcgac tcaggtcgta ttgttcgaga aatgtggttt
     2641 ccccacgttg gtgacgggtg agtttgtcac tgccatggac gatggaaacg agattctgcg
     2701 tttcggacag cacgtaatcc cttccccctg ccgcgacaag ttccccaaga ggctttccat
     2761 cgcactgaat cggatcaatg cctgccgcat ccaggcaggt gggcaataca tccacaagac
     2821 tgaccagttc ctgatgaacg cttccaccgc gatttcgagg tggtcgtatg aacaacggaa
     2881 ctcgaatgat atcctcaaag aaagcaccgt tctttcccca caagccatgg tgccccatca
     2941 tgtccccatg atcgcacgtg aagacaacca gaagattttc tccccagagc gcctttgcgc
     3001 gttcgagaat ctggccgata taatcgtcaa tctggacgac gttggcgtag tacatttgtc
     3061 gccattgtct ccagtaagag tcatcgaagt ttttctgcgc gcccccttcc gcggagtttg
     3121 aaccctctgt tccccccgga ccgtggtaac tgacacagtt actcttggtt ggatcatccc
     3181 attccccatc ccgtttgttt gccgcacgat ccttggacat atcaacacgc gaaagatatt
     3241 ccacaggcgt attcagcgga taatgcggtc ctgagaagga gacccacatg aaatgcggtt
     3301 ggttgttgtc aaggtggctc agatactcaa tcgcctttct gccaacccaa ctgtcgggat
     3361 gcatggactc gtcaccgggg aacagaaaga gattcttatc gcatcgcgat ccggttttgt
     3421 tgtgtaccaa atcacggtat tgggacatca ggccggcctt ttccaaatcc tttccgtagt
     3481 catcgtagaa ccatgcactg acctcattgc cgtcctgaag atccagatga tcgatgccca
     3541 agtccaggta gtactgacga aagtgatcat attcgcgagt gaagtctctc gacacatagg
     3601 ggtatggaac aggaatgaaa tggcacttgc cgtgcatgga tgtctggtag ccttgcgatc
     3661 ggaaaacctg gaaaacgttt ggaaggctca gtgacagcga cgctcctgtg tgatttgaaa
     3721 aagatcggga gcgatgcggg tacagaccgg tcgccagaga aacccgactc ggaagacaga
     3781 cgggacttgc ggcataggca ttctcaaacc gcatcccttc tcccgcaata cggtcaacgt
     3841 tgggcgtttt gtgcgaggtc gcaccacaac aactcaatgc ctcgcgatgc agttcgtcca
     3901 tggtgatcca gagaacgttc aaatcggtgt ttgccactgt aatgatccct tccgaatcag
     3961 catatttcag ggttcagcaa gcgcctgcca tgcgcttcga atttcctcgg gactcaatgc
     4021 ccgccgatac agtttgacct cgtcgagcag cccgacaaag tgtgcggcat gtttgacgtc
     4081 atagttgcca agtatgagat gaaagtcatt tgtattgatg gttcctggtc gctccatggt
     4141 tccacacaag acgccgtcca tatagagacg catagtcgtt ccgtcaaatg ttcccgccaa
     4201 atgcacccag cgtccaatgg gcagtggtgt gtctgagttg aggtggtgac tccaggaggt
     4261 catcggaatg tgaaaagagg gcttgccatc gagaacgccc atccgaaagc ccgtgtgggt
     4321 tccaccttgg agcactcgat tgagaaacca ggtgttattc tggccagggc ggtctgtctt
     4381 cacccagcag aggacactca gcgtcgaggt gatgttcagc atctcactgc tgggcacctc
     4441 aacacaacct ccatcacaca ccaatgcatt gcctgcaatt ccaggtgcgt attgtgcgtc
     4501 gcgcacgata ccacccaggc gatgtcctga ataatccaag gcaaccggac tgtcttcctc
     4561 ttcatcgaat gcccaccagg cgaccaagcc ttcttccgtt tcggaacctt tgaacgcttt
     4621 gcgtgacacc tgtctgcgaa gtgctttctc cgtttcctgg tgcgttcggt aaggtcggtc
     4681 gtagtcgccc aagccaatgt cttcaggaat caagtcttca tgggctgtcg gggcatccga
     4741 aaacacctcg cgtacagcgt ccaggtagcc gaatccgtac tcacgatagg gtgcgatgta
     4801 gtgaccctct ccccattcgt tccagttgtc caacagcagc atgcggctcc cgagctcgtt
     4861 ggctggaaat gtggcaatga tctcctttgc ctttgcgaga agcgttttga actcggttgg
     4921 cgggatcttc cagatcgtgc cttcgtctgc ccaaccactc cacgcctgtg aaacggtgac
     4981 tacctgtggg agaatgccga gcgcctgggt cttgttgatg tagtccacct gcgctgcgat
     5041 tgcttgctcc ggggtcgggt tgttgcccac ataccagcaa taggcgaagg tatagtccag
     5101 tccaatgcgc ttcatgagct cgagatgttt gggatcaagt ccacgatatt caccaagcag
     5161 atagagtcca tcaaaaccgg catcctgaca ggcttggcgc atgagatcga acgcttccaa
     5221 gacattctcc tcactgccca gatcctgaat cagaaactcc ggacggtaga tgaacagcag
     5281 cggcttgcca tccatcttca gataactcgg atgcttgaag tagttctcca tccagaaggg
     5341 aagcacgttc gtcgtcaggt catccaagtc tgccacaccc gaagtgcctc ggctctgatt
     5401 ttcccacatg atgcagaact tcatgtgatt gacgaaccgc gacttgaaca gcgcctcatg
     5461 gatggcgctg ccaaagcgca tcttgacagg ctcaccttga cttgctcgat accagcagta
     5521 caggaagaaa gaaacgccat gctccactgc ccacttggtc tcccagtcgg aaacctccgg
     5581 attctcctgg gcgtagaacc caagcgcggg ggttcgctca gggtgtttga gcagctgcga
     5641 ccacatgaag ggcttgtccg cttcccaaag gggacagtga tgggctccta caacgacgct
     5701 tgtcttgact ggattgggct caggaatgta ggacagcgat cttctctcaa cggcagaaag
     5761 gaagttgatg gacagtaccg cttcctccac acggtttgct gatacaaaac actcaaggcg
     5821 aagttcacga cggaaggcat cttcggcttc gatctcccag
//
LOCUS       DAOQUC010000044         5811 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_15206_length_5811_cov_3.107192, whole genome shotgun sequence.
ACCESSION   DAOQUC010000044 DAOQUC010000000
VERSION     DAOQUC010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5811
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15206_length_5811_cov_3.107192"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1113)
                     /locus_tag="PKH07_01360"
     CDS             complement(<1..1113)
                     /locus_tag="PKH07_01360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33626.1"
                     /translation="MDNTRSRATLSRGRQSWCVIFRHPLCFAPDGKQKLRVRRGLSTP
                     DEKRAKELVEQLNRILSDQSWWNLASREKAERFFDRKIVAAFYDPMSPSESDSWAVRE
                     EVIPMPGGRNPSDGYKRVLFVGTTGAGKTTIVRQLLGTDPKRERFPSISAAKTTTCDI
                     EIVLAEGLFRAVVTFIPRDRVRQYIVECVLAAVYAKLEDAPRSDLFRRFLEHSEQRFR
                     LSYILGNPTLLERELADKHDDEEEDLESETETNVEIAESERRELLANLNGYFETIGLL
                     AEETRLVMQETAIKMGIDMSNASDEEREDLRNQAEEQLGDFDAFHELVDSVLDDVETR
                     FEQLPEEEISRGRDGWPVKWSYTDSERSSFIETVNKF"
     gene            complement(1359..2120)
                     /locus_tag="PKH07_01365"
     CDS             complement(1359..2120)
                     /locus_tag="PKH07_01365"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNT33627.1"
                     /translation="MITFSLQSGSNGNSIYVETDGIRLLFDAGISGVQAQCRLAHHGR
                     DIQGVQAIILSHDHQDHVRCAGVCHRKFGIPIYATEPTFSSARRFLGKVKHVEHFRSG
                     DTIHFNGVCVHSIPTPHDGVDGVCFVVESKGKRLGVFTDLGHPFRSLREWFPELDAAY
                     LESNYDPDMLEIGRYPEYLKHRIRGEAGHLSNGEAADLVERFASPRLKWLALSHLSEE
                     NNTPQLAERETRKVVGKAFPLHVASRYSVGSLLTV"
     gene            2480..4177
                     /locus_tag="PKH07_01370"
     CDS             2480..4177
                     /locus_tag="PKH07_01370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33628.1"
                     /translation="MNQYLIVRCLQPENSHGLFTREILISEGMMGFHVVDLDAVGLPL
                     FESGDLVLLTRCSLRTAECEALYSAVERGVRLVCLQPPTGLAKRFGLVSTAEVLHPGW
                     IRVRQGHPGSRSPIQAHVPIALNEASGWDVLADAVSTDWVETGFPAVAMREVGAGKVA
                     FFFYDLSLAVARIRFGNPDLASHCTTGIWHWLHAMDLFTGHVDGRVRDIPQADFHGQL
                     LAWVLTDLCAVPLMRLWYYPRISHRSAAVFQSDDDGSTVAQFRELSDSLVKHSASGTF
                     YLMRDTLMTESDVSEFRALGHTFAPHANPFNSQEEIVFSFPEVLREETGLFQKRFGKC
                     SRTLQCHCAPWKGYMDWVPMFVESGYRLLFAYLSAPISFLNGFMCGSGRPMCFFGLDS
                     KQHDCWQQPVLIYDDTTVRDLISEKGLDEILASQADLIERTIQETHTAIGILSHPVSF
                     ATYSRPFIERGFDLLQQHNVPIFNGDQWLDFLQRRSRVQITLHDSHDEGLLYDIQNLD
                     GTMTLMVPSRQDAKDYRITMNDVLVDGFVERRMEQDYLLFEIEGSGERLTICVREDA"
     gene            4199..5341
                     /locus_tag="PKH07_01375"
     CDS             4199..5341
                     /locus_tag="PKH07_01375"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNT33629.1"
                     /translation="MNKVRIGLVGCGGRGISVTTLFRIHPQCEVTACCDIYEACATRA
                     AERFQIPARFVYTNYEKMLREAPVDAVFLAPDPMVQVAMACQAMQAGKHVCTEVPAAF
                     TLEECWKLIETVERTGMKYQLMEQTRYWGFVQTWKQMVDRGELGHVCLAQGEYVHYEE
                     HWGCWTDTETGDILPSNERPRDRDVLPHWRYLLLSDPIYYLPHTLSPLLSILGDRVLS
                     VACMGTRKQSYTQQGLPWCDIQYALMHTEKDTILLAGAGFSLPHVPRGATYCHWYELR
                     GTDGSVCSPRCYSEPFRVWRRGMSDFEAMSLSTIPLGADQEQAQTGHGGADYCPVDTF
                     IKSIIEDTTPPLDVYLTAELTAPAIVAAESARSGGALLKVPDFRRR"
     gene            complement(5379..>5811)
                     /locus_tag="PKH07_01380"
     CDS             complement(5379..>5811)
                     /locus_tag="PKH07_01380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33630.1"
                     /translation="FFLFKPSWRDSHLDARAANWASYHVPTTMTERLKDVPYQQPPWS
                     ERYPELTGILEDQPELPKGNVIRRNIHWGGDKWKYVEKVAEQLPTFESNLTEEDPLFA
                     DAAKMDFRLCRNSPAYELGFQKIPFEKIGIQPPRARKQRER"
BASE COUNT         1273 a         1518 c         1561 g         1459 t
ORIGIN      
        1 gaatttgttc accgtttcga tgaatgatga tcgttcggaa tcagtgtaac tccacttaac
       61 cggccatcca tccctgccac gtgatatttc ctcttcgggc agttgctcga agcgtgtttc
      121 tacatcgtca agaacgctgt ccacgagttc atggaatgca tcgaagtccc caagttgctc
      181 ttccgcctga ttccgaagat cttcgcgctc ctcatcagaa gcattactca tgtcaatacc
      241 cattttgata gccgtctctt gcatcacaag ccttgtctct tccgcgagga ggccgatcgt
      301 ctcgaagtag ccattgaggt tagccaggag ctctcttcgc tctgattctg cgatctcaac
      361 atttgtttcg gtttcggact ctaaatcctc ttcttcgtcg tcatgcttgt ctgccaattc
      421 cctctcaagc agtgttggat tgcccaagat gtaactgagt ctgaaccgct gttcactatg
      481 ctcaagaaag cgccggaaca agtcgcttct tggtgcgtct tcgagtttgg catagactgc
      541 tgcaagcacg cactcgacaa tgtactgacg gactcggtct cgagggatga atgtgacaac
      601 agccctgaac agcccctctg ccaatacaat ctcaatgtcg caggtagttg tcttggcggc
      661 ggaaatggag ggaaagcgct ctcgctttgg gtctgtaccc agtagctgtc tcacgattgt
      721 tgtcttgccc gcacctgtag ttcccacaaa aagaacacgc ttgtacccat cggaaggatt
      781 cctgccaccc ggcattggaa tgacttcttc tcggacagcc caggaatcag attccgatgg
      841 agacatcggg tcatagaatg cagcaacgat tttcctgtcg aagaatcttt cagccttctc
      901 tcgacttgcg agattccacc aagattgatc agagagtatg cggttcaact gctcaaccag
      961 ttctttcgcc cgtttctcgt ctggagtact aagcccccgc ctaactcgca gtttctgctt
     1021 gccgtcaggc gcaaagcaaa ggggatgacg gaagatcaca caccagctct gccttcctct
     1081 gcttagcgtt gctcttgacc ttgtgttgtc catagccaat acctcctgtt ttatgttgtt
     1141 ctgcgttgtt ccatataggc actgtatggg ctttcctcac gcctgtcaag caaaaaaaag
     1201 aacaacgcgg aacaacgcaa aggccatgct gagaattggg ctttccttcg ggtcgcctgc
     1261 agcactctcc aaggtgaaca gccacacaat ttcatgcttt ccatggcgaa ggagtggatg
     1321 ggggtttcgg caggcattgg acgacgtcag aaagcagact agaccgtcag cagcgaacca
     1381 acactgtatc gggatgccac atggagcggg aatgcttttc cgaccacctt acgtgtttcg
     1441 cgttccgcca gttggggagt gttattttct tcactgagat gcgacaaggc caaccactta
     1501 agtcgtggac tggcaaagcg ttccaccaga tctgccgcct cgccgttact caagtgtcct
     1561 gcttcaccgc gaatccgatg cttcagatac tcgggatagc gtcctatctc cagcatgtcc
     1621 ggatcatagt tggactccag ataggcagca tccagttcgg gaaaccactc cctcagagac
     1681 cggaagggat gacccaggtc agtaaacacg cccagtcgct tgcccttgct ttcaacgaca
     1741 aaacagaccc cgtccacacc atcgtgggga gtgggaatgg aatgaacaca gacgccgttg
     1801 aagtggatcg tgtcccccga gcggaaatgc tcgacatgct ttaccttgcc tagaaatcgg
     1861 cgtgctgaac tgaaggtggg ttctgtcgcg tagatgggaa tgccaaactt tctatggcag
     1921 actccggcgc agcggacatg atcctgatga tcgtgtgaga gaatgatcgc ctgaacaccc
     1981 tgaatatcgc gcccatggtg tgccagacgg cactgcgcct gtactccgct gatgcccgca
     2041 tcaaagagca gtctgatccc atctgtctca acgtatatgg agtttccgtt cgaacctgac
     2101 tgcagggaga aagtgatcat ctttcgtctc agttccttca ttcagaacca aagagaatac
     2161 tagttgaagt gctccaaaac gtccagcaga aagtgcaaca tgaattcgac cgcctctgca
     2221 ctcaaacgcc tctccagaat tggatcaagt cgtatctccg atcgaccaga tctgtgtcgg
     2281 ctatgctcgc ggtgctgtct ctgtttgact cgacagacat agattgatag ctttggggca
     2341 gtttgcacat gcaggcacat ggtttgtgct cacttgcatg ctgtgcttgc tcacgaaaga
     2401 aaccgactct tccggagtcc ggtttcccaa gagtgagtcc tcttcgtgcg tggaagggga
     2461 tcgagacgtg gagatgcaca tgaatcagta tctgattgtt cgttgtttgc aaccggagaa
     2521 cagtcatggt ctttttaccc gagaaatcct catttctgaa gggatgatgg gtttccacgt
     2581 tgttgatctc gatgcggtgg ggctgccgct tttcgagtcg ggagacttgg ttttgctcac
     2641 ccggtgctct ctcaggacgg ccgagtgtga agcgctctac agcgcggttg agcggggtgt
     2701 ccgtctggtt tgtcttcaac cgcctacggg attggcgaaa cgatttggct tggtgagtac
     2761 agccgaggtt cttcatcccg gatggattcg ggttcggcaa ggacatcctg gctcacggtc
     2821 tcccatccag gctcatgttc ccattgctct gaatgaggct tccggatggg atgtgctggc
     2881 agatgccgtc tcaacagact gggtagaaac gggcttccca gcagtggcta tgcgagaagt
     2941 gggtgcaggc aaggttgcat tcttcttcta cgacctttcg cttgccgtgg ctcggataag
     3001 gtttgggaat cctgatcttg cttctcactg cactaccggc atctggcact ggcttcacgc
     3061 catggatttg ttcaccggtc atgtggatgg ccgcgtgagg gacattcctc aggcagactt
     3121 tcatggacaa ctgctggcat gggttctgac cgatctctgt gccgttcccc tcatgcggtt
     3181 gtggtactac cccagaatca gtcatcgctc cgcagcagtc tttcaaagtg atgacgacgg
     3241 ctccaccgtc gcgcagtttc gtgaactctc tgactcgctt gtgaagcact ctgcatcggg
     3301 aacattttac ctgatgcggg acactctgat gactgagtca gacgtctcgg agtttcgagc
     3361 cctcggacac acttttgccc cgcatgccaa ccccttcaat agtcaggagg aaattgtctt
     3421 cagctttcct gaagtacttc gcgaggagac aggtctgttc cagaagcgat ttggcaaatg
     3481 cagccgaacg ctgcagtgtc actgcgctcc atggaaaggc tacatggact gggtgccgat
     3541 gttcgttgag tcgggatacc gacttctctt cgcataccta agcgcaccga tttccttcct
     3601 gaacggcttc atgtgtggtt cgggcaggcc aatgtgcttc tttggtctgg acagcaaaca
     3661 gcacgactgc tggcagcaac ccgttttgat ctatgacgat acgaccgtac gggacttgat
     3721 cagtgagaag ggcttggatg agatacttgc cagccaagct gatcttatcg agaggacgat
     3781 tcaagagacc cacacggcga tagggattct gtcccatccg gtcagtttcg ccacctacag
     3841 caggccgttt atcgagcgag gcttcgatct cttgcagcag cacaatgttc cgattttcaa
     3901 cggggatcaa tggctcgatt ttctgcaacg acgcagtcgc gtgcagatta ctctccatga
     3961 cagtcatgat gagggactgt tgtatgatat ccagaatctc gatgggacca tgacactgat
     4021 ggtgccgtca cggcaggatg cgaaggacta tcggatcacc atgaatgacg ttcttgttga
     4081 cgggtttgtc gaacgtcgca tggaacagga ctatcttctc tttgagattg aggggtcggg
     4141 agaaaggctc acgatttgcg ttcgtgaaga cgcatgatag acagtgagga gtagcgcagt
     4201 gaacaaagtc agaattgggc tcgttggctg tggcggaaga ggaatctctg tcacaacgct
     4261 ttttcgaatt catccacaat gcgaagtgac ggcttgttgc gacatctatg aagcctgtgc
     4321 cactcgcgct gctgaaagat tccagattcc cgcacgtttt gtctatacca actatgagaa
     4381 gatgctgcgt gaggctccag tggatgccgt ctttctagcc cccgatccga tggtgcaggt
     4441 tgccatggca tgtcaagcaa tgcaggcggg caaacacgtt tgtacggaag tacctgccgc
     4501 gtttaccctc gaagagtgct ggaaactcat cgagaccgtc gaacgtaccg gcatgaagta
     4561 ccaattgatg gagcaaaccc gctactgggg ttttgtccag acctggaagc agatggtgga
     4621 caggggagaa ctcggccatg tctgtctcgc tcaaggggag tatgttcatt atgaagagca
     4681 ttggggatgc tggacggata cagagacggg cgacatactg ccctcgaatg agagacccag
     4741 agatcgcgat gtgctgcctc actggcgcta tctgctcctg tctgatccaa tctactacct
     4801 tcctcacacg ctgagtccgc tgctgagtat tctcggagat cgggttttga gcgttgcctg
     4861 catgggaacg cgaaagcaga gttacaccca acagggtctt ccgtggtgtg atattcagta
     4921 tgcgttaatg cacacagaaa aggataccat tctgttggca ggggcgggat tcagtctccc
     4981 ccatgtgcct cgcggggcga cttattgtca ctggtacgaa ctgcgtggca cggacggcag
     5041 tgtgtgttcg ccacgttgct actccgagcc atttcgggtc tggcgaaggg ggatgagtga
     5101 tttcgaagcg atgtcactgt caacgattcc gcttggtgca gaccaggaac aggctcagac
     5161 aggtcatggt ggtgcagatt actgtccagt ggatacattc atcaagtcaa ttatcgaaga
     5221 cacaactccg ccattggacg tctatctgac tgcggaactg actgcgcccg caattgtcgc
     5281 ggcagaatcg gctcgaagtg ggggagcact ccttaaggtg ccagactttc gccgcaggta
     5341 ggctgtccga cggtgtatga aatccgcgga tcccacattc aacgctccct ttgcttcctg
     5401 gctcgaggcg gctgaatccc aatcttctcg aagggaatct tctggaaccc cagttcgtat
     5461 gcaggcgagt ttcggcaaag tcggaaatcc atcttggcgg cgtctgcaaa gagtggatcc
     5521 tcttcggtga gattggattc aaatgtggga agctgttctg ccactttctc gacgtacttc
     5581 catttgtcac cgccccaatg gatgttgcgc cggatgacat tgcctttcgg aagctccggt
     5641 tgatcctcaa gaattcctgt cagttcagga taccgttcgc tccaaggcgg ttgctggtat
     5701 ggaacatcct tcaggcgctc ggtcattgtt gtcgggacgt ggtaacttgc ccagttggct
     5761 gcacgcgcgt ccagatgaga gtctcgccag gaaggtttga agaggaagaa g
//
LOCUS       DAOQUC010000045         5795 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_15262_length_5795_cov_3.204704, whole genome shotgun sequence.
ACCESSION   DAOQUC010000045 DAOQUC010000000
VERSION     DAOQUC010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5795
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15262_length_5795_cov_3.204704"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>544
                     /locus_tag="PKH07_01385"
     CDS             <1..>544
                     /locus_tag="PKH07_01385"
                     /inference="COORDINATES: protein motif:HMM:NF013377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase family glycosidase"
                     /protein_id="HNT33631.1"
                     /translation="GDEEKALRYEAARSVIVKAIQENLWDSQKKFFGDLHVPSNCLET
                     TYAVSGFWPLAAGAATESQAANLVKHLTDPELFWTPFPVSTVAVKEAGFDLDCWRGPV
                     WVSQNLWLVLGLRRYGYLQEAAQLVIKTLEGMQKVWDRDRKIYEFYHPQEFFPDKLTR
                     KGKDYGPVVYYTGHNPLHAIV"
     assembly_gap    545..554
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <555..756
                     /locus_tag="PKH07_01390"
     CDS             <555..756
                     /locus_tag="PKH07_01390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33632.1"
                     /translation="FHGLWGIEPCVGGFVWRPPQALFSLSGSVRFHWGDELMELASDG
                     KGTVTCNGTDLIAGQRLIAAPR"
     gene            complement(794..3088)
                     /locus_tag="PKH07_01395"
     CDS             complement(794..3088)
                     /locus_tag="PKH07_01395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33633.1"
                     /translation="MAAKMITSLLSLAVLSLMPLKSYADDMHPEKVSQLLANGGFESG
                     SDGSGVPLFWSTYVPGATAEFRVVSEHARQGNHALSIRTTSDVKAILVSEPVPVAPGE
                     ILNLSVWCRCGAIETASSGSITLSVGFLNRDRRYFSWHRTQNIPESHDTWFDWTTQAT
                     VLPGVAYATFQIGINKMKGELWIDEAQMHTTSSLAVRFDLAELTSSVGLVPLPLILIN
                     RDPQLKGKTVVLTTEPGNHTQHIALSGERETPLSGDFTMPKRGKQTVRAILESEAKER
                     HCVAEETVTIPEILRVEPLVPTHFCVEDGTPRLEGRLWVYDQQQKRDATKISLVLKTG
                     ESPVRQIHITNLPPNPVLYNIDVADATPGDYVLDVSVLQEGQIVASATQDWHIVQRAS
                     SQVMVGADGYLIVEGKPFFPIGMFNGFRLSEQVDAGFNVTHGYNAMATTPETVSNNQR
                     AKDFLDETHKLGMKTLMLVTHGGHHRPVDDEFVRRVRMFRNHPALLAWDEEEGVARGE
                     MPLSNLEEMHTLLKREAPEHPFMVGDARAPIFEVHDRSYLFPAEFMDLGMWWWYPFPL
                     DLNNNASVLDGEEDFHSNLLEPPSFLTLAKTNKPIWVGIQAYKKPNRTDGRFPTPTEY
                     RAQAYLSIIHGAKGLMYYVGSGSGGNGILNKPDEGNWNYLKELVKELRSLEDVIMSPN
                     ANQQIRVLSEDAPVSALLKKTARGYVLLAANRSPNTVRARFELDPSVPVTAEVLFEDR
                     TAELLQNVLEDTFTPLGVHLYRFR"
     gene            3247..5673
                     /locus_tag="PKH07_01400"
     CDS             3247..5673
                     /locus_tag="PKH07_01400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33634.1"
                     /translation="MKRLAMCWDYFPVFAALMLFASSGQSQEAKMKAVVLDRFNGETL
                     WSVADAEQSQGTGRIETTKWGSILSYDFAKETTRVGIAVERLVVPGRPEDFEIQTYGD
                     LKGHSLVLRLSDARGHRFEKHLVSCDDLLSTGARNHVVAWDGGRDWVHFSDLLDVTPI
                     LPISVSEIVLEASRGASLQGSVSLLQLAAHTWATDSECVRVSFPQTKHRGLFLLNSFS
                     SFFPFQIPIRVENLSSEAYRAEATIQILDGDGFAVGSVEWPIEVPGLGEYDDPIELEP
                     DRLGFFSLVLSGVGAVPVRKDFVLSRESSSQSRSSSFFGVNIGLTSLESPRERRFAAS
                     RAAQAGVSWTRETFHEVSPEAERGFLDLQRYDDSIDIAEREGIHVVGELVYGVSPLSP
                     FAVEDAEVSDFERWRRWVSAVVTQYQEDIKYWEIWCSSERVVASSDALLFYEHLKQAH
                     ETIKATDPEEYVVGAFLTSAGLEFLESLLLAGGGAYWDVLSVIADPQDVGDASLPEQI
                     AGVRALLQKHGCTKPLWVTCSSTMDNSVQDGMAPRLQAESLARMYLHCSSIEGVQKVF
                     WNRFRDIDAESGVQGVTSGLLRSDSVPKPAFLALKTVAEHLAGSVFEATVALGLESDV
                     HALSYLQDNSRILAVWSEGDPLNIAMAVGSVPVTVCDLWGHRTRLASHKGCILLPVTR
                     SPLFLRFVPPISFSSDMVHSLKQYPLSLTQGQETQISMELPLLPDGKWEGAASVEATD
                     GLLVNSPASSLELRENLSKTIVSATIQVQPGCRIGDHLIHWEFRSAHLPFGWTLQQVS
                     VTPDESEAAP"
BASE COUNT         1263 a         1415 c         1605 g         1502 t
ORIGIN      
        1 ggcgacgaag agaaagcact tcggtatgaa gcggcacgta gtgtcattgt gaaggcgatt
       61 caagagaatt tgtgggactc ccagaagaag ttctttggtg accttcatgt gccgtcaaac
      121 tgtttggaaa ccacctatgc agtgtcaggc ttctggcctc ttgcggcggg agcggcaaca
      181 gagagtcaag cagcaaatct tgtcaagcat ctcacggatc cggagctttt ctggacacca
      241 tttccagtca gcacggttgc ggtcaaggag gctggtttcg atctggattg ctggcggggt
      301 cccgtctggg tgtcgcagaa cctctggctt gtgcttgggt tgcggcgcta cggttatctg
      361 caagaggctg cccagcttgt gatcaagact ctcgaaggaa tgcagaaggt ctgggatcgg
      421 gatcgcaaga tttacgagtt ctaccatcct caggagttct ttccagacaa actgacgcgc
      481 aagggcaaag actatggtcc cgttgtctac tacacgggac acaatccatt gcatgccatc
      541 gtttnnnnnn nnnnttttca tgggctgtgg ggcatagagc cttgtgttgg aggtttcgtc
      601 tggaggcctc cacaggcgtt gttctcactt tcgggcagtg ttcgatttca ttggggagat
      661 gagttgatgg aattggcatc agacgggaag ggaaccgtca cctgcaatgg aacagacctt
      721 attgcgggac agcgtctcat cgctgctccg agataggttg tctatcgcag acggctcgtc
      781 gaggcagact ctatcacctg aatctgtaca ggtgaacgcc gagaggtgtg aatgtgtctt
      841 cgagcacatt ttgaagcagt tcggcagttc gatcctcaaa gagaacttct gctgtcaccg
      901 gcaccgaagg atccagttca aatcttgccc gaaccgtatt gggtgagcgg ttggcggcaa
      961 ggagaacgta gcctctggcg gttttcttca gcaatgcact gaccggcgca tcctccgaca
     1021 ggactctgat ctgctgattt gcgttgggag acatgataac atcttcaagt gagcgcagtt
     1081 ccttcaccaa ctccttcaga tagttccagt ttccttcgtc aggcttgttg aggattccat
     1141 tcccacccga tcccgatccg acgtagtaca tcaaaccctt tgccccatgg atgatcgaga
     1201 gataagcctg tgcccgatac tctgttggtg ttgggaaacg cccatcggtt ctgttgggtt
     1261 tcttgtaggc ttgtatgccg acccagatgg gcttgtttgt cttagcaagt gttaggaaac
     1321 ttgggggttc caacaggttt gaatggaagt cttcttcacc atcgaggaca ctggcgttgt
     1381 tgttgagatc caacggaaac ggataccacc accacatccc caaatccata aactcagccg
     1441 gaaacagata tgaacggtcg tgaacttcaa agattggcgc tctcgcatcg cccaccatga
     1501 acgggtgctc aggtgcctca cgcttgagca gagtgtgcat ctcttcgaga ttcgaaagag
     1561 gcatctctcc gcgtgcaacc ccttcctcct cgtcccaggc aagcagtgcc ggatggttgc
     1621 gaaacatcct gacgcggcga acgaactcat catccacagg ccgatggtgt ccaccgtgtg
     1681 tgacgagcat cagtgtcttc atcccaagtt tgtgtgtttc atcaaggaag tccttggctc
     1741 gttgattgtt ggagacggtt tctggggttg ttgccatggc gttatatcca tgggtgacat
     1801 tgaaacccgc atccacctgt tctgacaatc gaaaaccatt gaacattccg atagggaaaa
     1861 agggttttcc ttcgacgatg aggtatccat ccgcgccgac cataacctgc gatgaggctc
     1921 tctggacaat gtgccagtcc tgtgtcgcac tcgcgacgat ttggccttcc tggaggacag
     1981 acacgtcaag cacgtagtct cccggagtgg cgtctgctac gtcaatgtta tagagaacag
     2041 gatttggggg gagattggta atgtgaatct gccttactgg agattctccc gttttcagaa
     2101 caagagaaat cttggtggca tcacgtttct gctgctgatc gtacacccaa aggcgtccct
     2161 ccaatctggg tgtaccatcc tcaacacaga aatgggtcgg cacaagcggt tcgacgcgga
     2221 gaatctcggg aatggtcacc gtctcctcag cgacgcaatg cctctctttg gcttcacttt
     2281 cgagtattgc tctgactgtc tgctttcccc tctttggcat cgtgaagtct ccacttaggg
     2341 gcgtttctct ctcacctgag agtgcgatgt gctgggtatg attcccgggc tcagttgtga
     2401 ggacaacggt tttccccttg agttgcggat cgcgattgat gagaatcaaa gggagcggca
     2461 ccaaaccgac tgatgaagtc aactcagcaa ggtcaaagcg aacagccaac gatgaggttg
     2521 tgtgcatctg cgcctcatca atccacaact cacctttcat tttgtttatc ccgatctgaa
     2581 aagttgcata ggccactcct ggcagcaccg ttgcctgtgt tgtccagtca aaccaggtgt
     2641 catgactctc gggaatgttc tgtgtcctat gccaagaaaa gtagcgccgg tctcgattca
     2701 gaaaaccgac acttaaggta atacttcccg atgaagcagt ctcgattgcc ccacaacggc
     2761 accacacact caggttcagt atctcaccgg gagcaacggg aacgggttcg ctgaccagaa
     2821 ttgccttaac atcgcttgtg gtccggatag agagggcgtg attaccttgc cttgcatgtt
     2881 ctgaaacgac tcgaaactca gccgttgctc ctggaacata ggtactccag aagagcggaa
     2941 caccactgcc gtcagaaccg gattcaaaac cgccgttggc aaggagctgg gagaccttct
     3001 caggatgcat gtcatctgca tagcttttca aaggcatcag ggaaagaact gctaatgaga
     3061 ggaggcttgt gatcattttg gctgccatgg cagagattcc tcctgcggcc aagtgttgac
     3121 tcagattcta gcggagtgat cccatggaaa caagtcggtg cgcgaaagaa attcccttag
     3181 aacttgtctg ccaagtccat attcgataga gttatccaga tgtttggctt tgtgaggttt
     3241 tcttcgatga agaggttggc aatgtgttgg gattacttcc ctgtctttgc ggcattgatg
     3301 ctgtttgcct catcaggtca atcacaggaa gcgaaaatga aggccgttgt gcttgaccgc
     3361 ttcaatgggg agacgctttg gagtgtggcc gatgcggagc agtcacaagg taccgggaga
     3421 attgagacga cgaagtgggg aagcattcta agttatgatt tcgcgaaaga gacaactcgt
     3481 gttggtattg ctgtggaaag gctcgttgtt cctggcaggc cggaagattt tgagattcag
     3541 acctacggtg atcttaaggg gcattctttg gttcttcggc tttcggacgc tcggggacat
     3601 agattcgaga agcaccttgt gtcatgcgat gatcttctct ccaccggtgc cagaaaccat
     3661 gttgtggctt gggatggggg acgagactgg gttcattttt ccgatctgtt ggatgtgaca
     3721 ccgattcttc ctatttccgt atccgagatc gttctggagg caagccgcgg tgcatccctg
     3781 caaggatcag tgtctctgct tcagcttgct gcccacactt gggcgactga ttccgagtgt
     3841 gttcgagtgt ctttcccaca gacgaagcac agaggtcttt ttctactgaa cagtttctcc
     3901 tctttctttc catttcaaat cccaatacgt gttgagaacc tctccagcga ggcctatagg
     3961 gctgaggcaa caatccagat tcttgatggc gacggctttg ccgtaggttc ggttgaatgg
     4021 cctatcgagg tgcccggcct tggagagtat gacgatccga tcgaacttga accagaccgc
     4081 ttggggtttt tcagtctcgt gctctccggc gttggagctg ttcccgtacg taaggatttt
     4141 gtgctgtctc gggagagcag ttctcaaagt agaagcagct cttttttcgg agtcaacatt
     4201 ggtctgacat ctctggagtc cccaagggag cgtcggtttg cggcatctcg ggctgcgcag
     4261 gctggagtgt cctggacacg agaaacattc catgaggtat cgcctgaagc cgaacgcggg
     4321 tttctcgatc tgcagcgcta tgacgattcg atcgatattg cagagcggga aggaattcac
     4381 gttgtcggag aactcgttta cggagtctcc ccattgtccc ctttcgcagt tgaagatgca
     4441 gaggtatccg attttgagcg gtggcgcagg tgggtttccg cagtcgtgac gcaataccag
     4501 gaagacataa agtattggga aatatggtgc agttctgaac gagtggtggc gtcttcggat
     4561 gctcttctgt tctatgagca tttgaagcag gcgcatgaga caatcaaagc gacagatcct
     4621 gaggaatatg tggttggtgc ctttctgacc agtgcgggtt tggagtttct tgaatccctg
     4681 ctcttggctg gcgggggagc gtattgggac gtccttagtg tcattgctga tcctcaagat
     4741 gtcggcgacg cctcattgcc ggagcagatt gccggagtgc gcgcacttct gcagaagcat
     4801 ggctgtacga aacccctttg ggtaacgtgc tccagtacaa tggacaattc tgtgcaggat
     4861 ggcatggctc cacggttgca ggcagagagt cttgctcgta tgtatctaca ttgctccagc
     4921 atcgaaggag tgcagaaggt cttttggaat cggttcaggg acattgatgc tgagtccggt
     4981 gttcagggtg tcacttccgg attgcttcga tctgattcag tcccgaaacc ggcgttcctg
     5041 gctttgaaga ctgtggcaga gcatctcgct gggagcgttt tcgaggcaac tgttgctctt
     5101 gggttggaat ctgatgtcca tgcactttcc tacctgcagg acaacagcag aatccttgca
     5161 gtctggtctg agggagaccc gctgaacatt gccatggctg ttggtagtgt tccggtaaca
     5221 gtgtgtgatc tttgggggca tcgaaccaga ttggcatccc acaaggggtg tatcctcctg
     5281 ccagtgacca gaagtccgtt gtttctgaga tttgtgccac cgattagttt ttcatccgac
     5341 atggttcatt cgctgaagca gtatcccctg agcctgacac aggggcaaga gacacagatc
     5401 tccatggaac ttccgttgct gcctgatggg aagtgggaag gtgccgcgag cgttgaggca
     5461 acggacggat tgcttgtcaa ctcacccgct tcgtcgcttg aacttcggga gaacttgtca
     5521 aagaccattg tatccgccac aattcaagtg cagccgggct gccgaattgg tgatcacctg
     5581 attcattggg aattccgaag cgcacatctt ccattcggtt ggacgttaca gcaggtttct
     5641 gtcacgccgg atgaaagcga ggcagcccca tgatccttcc ctatcgcgac gagaatccct
     5701 ccaggaagtt tcccgcgttg acctatcttc tgattctgat caatcttggg gtgttcatct
     5761 atcaagtctg gcttccttca ggtcaacagg aagcg
//
LOCUS       DAOQUC010000046         5794 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_15268_length_5794_cov_3.014462, whole genome shotgun sequence.
ACCESSION   DAOQUC010000046 DAOQUC010000000
VERSION     DAOQUC010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5794
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15268_length_5794_cov_3.014462"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(115..1620)
                     /locus_tag="PKH07_01405"
     CDS             complement(115..1620)
                     /locus_tag="PKH07_01405"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNT33635.1"
                     /translation="MMNRPRPNVLILISDQHNPHIAGFEGDKRARTQHLDRLAAESVR
                     FTSAYCQCPLCTPSRISLWTGMLPWRCAAWGNNMPIFPEFCTLPEHFGRHGYATCGIG
                     KMHVGGERPMNGFHERPYGDLIPNCFCGHQPDPIHSAKDQNWTNHQVGRFTYAGATEV
                     PESLIQDHVVSCQAAAWIRDHAATSKDKPWFVCASYQRPHHPLTAPSRYFKRYWPQGS
                     PPLGKLPDGYPDNIHPHDRFIVEDFNLAKLPREEIQRGLAAYYASVDYLDDCIGDLLN
                     SLESEGLLKNTVVVYLSDHGDLASEYGLWWKRSPREGSARVPLLIRVPDNPTIGQVDT
                     PVELVDIFPTLCDVCQIPKPRDLDGETLLPFLHGSGERRRKDFARSEYLLRSESCFRM
                     IRTPRWKYVEFLIEDWQPMLFDMQEDPQELSNIAAEPTHQGLIAELHERLWSDGQNWE
                     SLLNEKRLDLERAQKEKYTHYDCSPNQFALPDGTLINAEMEFYRQFLRDMA"
     gene            complement(1785..2735)
                     /locus_tag="PKH07_01410"
     CDS             complement(1785..2735)
                     /locus_tag="PKH07_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010583349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate isomerase"
                     /protein_id="HNT33636.1"
                     /translation="MDFNTTVKGSLLEGFFPKGWDLKAFDRICSQPPSKATQREPWWN
                     KKFSPLACESLEDFNTFMGHEIALQIAETRKNGQQLAIILPVGPMGMYRWTVFFLKEW
                     GVPCDHVHGFNMDEWSDGKGNTLPGNEAGSFQDAMEQAFYGPLGKLTIPKDQRHFATK
                     SSLPKYPEQIAALKNNGAKLAVVYGIGRVMHIAFWEPHFAAEFSSVQEWKSQPFRLGA
                     QLHPLTIEQNAITSFKSRTTLVPCRANTIGPGLFLQADWAIGGADGTLGRGMMWQGLS
                     FWVTLHHKPSTWIPSTWMPTLPGKLFFLKELAGPLVAECN"
     gene            complement(2758..3798)
                     /locus_tag="PKH07_01415"
     CDS             complement(2758..3798)
                     /locus_tag="PKH07_01415"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNT33637.1"
                     /translation="MAKVGWGVIGAGGIADRRTIPEGILPAKNAKLVAVMDADPETAQ
                     TLSSKYQVPSYIRAADLLANEQVEAVYVASPTYCHKPQTIEAARAGKHVLCEKPLGLT
                     IREAEAMLDECRKAKVNLGVGFMMRYNVYHQKIRSLIEKGSIGTVVFARAQLTCWYPP
                     IEGAWRQDPKLGGGGALMDLTVHCVDLLEFLLGPVAEVGSLVERRVHAYPVDDTNAVM
                     IRFASGAVGFVDCAFNIPDDASENVLEIQGSKGCIKGKMTIGQGPGGEVVQCLLKNAG
                     GYSASQKREPVTYQPLALSVKNTYQSEIEAFSDSVLKAVPPPISGDDGLHNMKVVEAV
                     KKSAKTGRFQKV"
     gene            complement(3822..5096)
                     /locus_tag="PKH07_01420"
     CDS             complement(3822..5096)
                     /locus_tag="PKH07_01420"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004625557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNT33638.1"
                     /translation="MAEKLAIHGGKKTVTKPFPGWPQFSEATKKAALEPLENGRVNYW
                     TGVKGMEFEKKWAQYNGAKFCISTSNGTSALHVALAAAGIGPGDEVIVPSYTFIASSF
                     AIVQAGAIPVFADVNKADHCISAKDVESKITRRTKGIVVVHLYGNICEMDPIMKIAAK
                     HNLFVLEDCAQAHGGEYKGKKIGTIGHAGAFSFCQSKAFTTGGEGGAVITNDDSLAWE
                     CRSFRDHGYDVRERLRLLELEQKLPYIHNRVGFNYRMTEIQSIIGIHELARFDRWNLK
                     NRQRNGQILIDALKDVPEILYLPVHNEKRKNGFFVFPITLKKERMKCTLKEFLAALSA
                     EGVPNHPVFWPQCYNERAYTEHNGFGKAKFPFESKEYTSPKSVQYDKVECPNAAWHQD
                     RTFITLVHPTLTPYHMNLFAKGIKKVIAAFAK"
     gene            complement(5598..>5794)
                     /locus_tag="PKH07_01425"
     CDS             complement(5598..>5794)
                     /locus_tag="PKH07_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33639.1"
                     /translation="TGAMTEFWADNQKIFETDILQDNLFNQIRVGAPEGQIGTAYFDN
                     IQLEVGYASSGVRESDWASY"
BASE COUNT         1229 a         1590 c         1542 g         1433 t
ORIGIN      
        1 ctgactggga tcgtctctaa gacgagatat ctggtaacca aactttgggg agccgagcca
       61 ttggttcggc tcctcttatt tccttttcct gccagtctga actgagagtc ggatctatgc
      121 catatctctg aggaactgac ggtagaactc catctctgcg ttgatgagag tgccgtcggg
      181 cagtgcaaac tggtttgggg agcaatcgta gtgcgtgtac ttttccttct gtgcacgttc
      241 aaggtcgagt ctcttctcat tcaggagact ctcccaattc tggccatcgc tccagagtcg
      301 ttcgtggagt tccgcgatca gaccttggtg tgtcggctct gcagcaatgt ttgagagctc
      361 ctgcggatct tcctgcatgt caaagagcat cggttgccag tcttcgatca ggaactcgac
      421 gtacttccat cttggtgttc ggatcatccg aaagcagctc tccgagcgaa gcagatactc
      481 gcttcgggcg aaatctttcc ggcgtctttc tcccgatccg tgcaggaacg gaaggagtgt
      541 ttcaccgtcc agatcccggg gcttggggat ctgacacacg tcacagagtg ttgggaagat
      601 gtctaccaac tctaccggtg tgtccacctg cccgatggtc ggattgtccg gcactctgat
      661 gagcagcggg actcgtgctg agccttcacg cgggcttctc ttccaccaaa ggccgtattc
      721 tgacgccaga tcgccgtggt cggaaagata gacgaccacc gtgttcttaa gcaggccttc
      781 cgattccaga ctgttcagca agtctccgat gcagtcatcc agataatcta ctgaagcata
      841 gtaggctgcc aagcctcttt ggatctcctc gcgaggaagc ttggcgaggt tgaagtcctc
      901 aacgataaag cggtcatggg gatgaatgtt gtcggggtat ccatcgggaa gctttccgag
      961 cggcggtgac ccctgaggcc agtagcgttt gaagtaccga ctgggtgctg tcaaggggtg
     1021 atgcggtctc tgatatgacg cacagacaaa ccagggcttg tctttgcttg tcgctgcgtg
     1081 atcccgaatc catgccgctg cctgacagga aaccacatga tcttgaatca ggctttcggg
     1141 gacttccgtt gcccccgcat aggtgaatct gccaacctga tggtttgtcc agttctggtc
     1201 ctttgcggag tgaatcggat ccggctggtg gccgcagaag cagttgggaa tcaaatcccc
     1261 atagggtctc tcatgaaatc cgttcatcgg tctttcgcca ccgacgtgca tctttccgat
     1321 gccacaggtt gcgtatccgt gtcggccgaa atgctcggga agcgtgcaaa actcaggaaa
     1381 gataggcatg ttgttgcccc aggccgcgca gcgccaaggc agcattccgg tccacaggct
     1441 gatgcgagac ggggtgcaga gtggacattg gcagtaggca gaggtgaaac gaacggactc
     1501 cgccgccaag cggtcaagat gctgagtccg tgctcgttta tctccctcga aaccagcgat
     1561 gtgaggattg tgttgatccg agataagaat cagaacgttt gggcgtggcc ggttcatcat
     1621 cttctccttg acacagtaga tccgatgttc accggaattg ttcccacgga atgcacataa
     1681 ttgcaagcga agatcacggc gtgctacaaa tcaagcaccc gcgcgcagat ctacagagac
     1741 cctacgtgcg ggtgctatgg tgaagaatgt tggtgctgaa ctgcctagtt gcactcggcg
     1801 accagggggc ctgccagttc tttcaggaag aacaactttc cgggtaatgt tggcatccag
     1861 gtgctgggaa tccatgttga cggtttgtgg tgcaaggtga cccagaagga cagcccctgc
     1921 cacatcatgc cacgtcccag tgtgccatcg gcgcctccaa ttgcccagtc ggcctgcagg
     1981 aaaaggccgg gcccaattgt gttggcacgg cagggcacca gcgttgtgcg gctcttgaac
     2041 gaggtgatgg cattctgttc gatggtcagc ggatgaagtt gtgcgccaag tcgaaacggt
     2101 tggctcttcc actcctggac gctgctgaac tctgcggcga aatgcggttc ccagaatgca
     2161 atgtgcatca cgcggccgat tccatagaca acggccaact tggcgccatt gttcttgagc
     2221 gcagctatct gttcgggata cttcggcaat gagctctttg ttgcgaaatg gcgctgatcc
     2281 ttcgggatgg tcagcttgcc cagggggccg tagaatgcct gttccatagc atcctgaaat
     2341 gagcccgctt cgttgccagg cagcgtattt cccttgccgt cgctccattc gtccatgttg
     2401 aagccgtgaa catggtcaca ggggacaccc cattccttca ggaaaaaaac ggtccagcga
     2461 tacattccca tgggacccac gggaaggatg atcgccaatt gctgtccgtt cttgcgcgtt
     2521 tccgcaatct gaagagcgat ctcgtgaccc atgaaggtgt tgaaatcctc caatgattcg
     2581 catgcaaggg gggagaactt cttgttccac cacggctcac gctgagttgc tttgcttggg
     2641 ggctgtgaac agattctgtc gaacgccttc aaatcccatc ctttggggaa gaagccttcc
     2701 aagagggatc ctttgaccgt tgtgttgaaa tcaatcgcca tgatgtgctc cttatctcta
     2761 gaccttctga aaccgccctg tctttgccga cttcttgact gcctccacga ccttcatgtt
     2821 gtgaaggcca tcgtcgccgc tgatgggtgg tgggactgct ttcaaaacgg aatcggagaa
     2881 cgcttcaatc tcactctgat atgtgttctt gacactgagc gccagaggtt ggtaggtgac
     2941 gggctcacgt ttttgtgaag cgctgtagcc tccggcattc ttgagcaggc actgcaccac
     3001 ttcaccgcca ggcccctgac cgatggtcat ctttcccttg atacagccct tggagccctg
     3061 aatctcaagc acgttctcac tggcatcgtc gggaatgttg aacgcacagt ccacaaatcc
     3121 aactgcgcct gaagcaaacc gaatcatgac cgcgttggtg tcatccaccg gataggcatg
     3181 aactctgcgt tcgacaagac ttccgacttc agccacaggc cccagaagga actccaacaa
     3241 gtccacgcaa tgcaccgtca agtccatcag agcgccaccg ccacccaatt ttggatcctg
     3301 ccgccacgct ccctcgatcg gtggatacca acaggtcaac tgtgcccgcg cgaaaaccac
     3361 ggttccaatg ctgccctttt cgatcagcga ccggatcttc tggtggtaga cgttgtagcg
     3421 catcatgaaa cccacgccga gattcacttt tgctttgcga cattcgtcga gcatggcttc
     3481 ggcttctcgg attgtcagcc caagtggttt ctcacacagc acatgtttgc cggctcgtgc
     3541 tgcctctatc gtctgcggct tgtgacaata ggttggcgat gcaacataaa ctgcctcgac
     3601 ctgctcgttt gcaagcagat cggccgctcg aatgtacgag ggcacctgat acttgctcga
     3661 aagtgtctgc gctgtttcgg gatcagcatc catcactgcg accaacttgg cattctttgc
     3721 gggcaggatt ccttccggga tcgtcctgcg atccgcaatg ccaccggcac caataactcc
     3781 ccaaccgact ttagccattt gggttctccc ctttctcgat cctacttcgc gaaggcagca
     3841 atgaccttct ttatcccctt ggcaaagaga ttcatgtgat aaggcgtcag tgtcggatgc
     3901 accagtgtga tgaatgtccg atcctgatgc catgctgcat tgggacactc aactttgtca
     3961 tactgaacgc tcttgggtga agtgtattcc ttgctttcga acgggaactt cgctttgcca
     4021 aaaccattgt gttcggtgta cgcgcgctcg ttgtagcact ggggccagaa cacaggatga
     4081 ttcggaacgc cttccgcgga cagggctgcc agaaattcct tcaaggtgca tttcatgcgt
     4141 tcctttttca gtgtgattgg gaacacaaag aaaccgttct tgcgcttctc gttgtgaaca
     4201 ggcaggtaga gaatttcggg aacgtctttc agtgcgtcaa tcagaatctg tccattgcgc
     4261 tgacgattct tgagattcca tcggtcgaat ctcgccaact catgaatgcc gatgatggac
     4321 tggatttcag tcattcggta gttgaaaccg acgcgattgt gaatgtaggg aagtttctgt
     4381 tccagttcca gaaggcgcag tcgctctcgg acatcgtagc cgtgatctcg gaacgagcgg
     4441 cattcccagg cgagactgtc atcattggta atgaccgcgc ccccttcacc acctgtcgtg
     4501 aatgccttgc tctgacagaa actgaacgca ccagcatgtc caatcgttcc gatctttttc
     4561 cccttgtact cgccaccatg agcctgggcg cagtcctcga gcacaaacag attgtgcttc
     4621 gccgcgatct tcatgattgg atccatctca cagatgttgc cgtacagatg cacgacgaca
     4681 atacctttgg tgcgtctggt gatcttgctc tcgacatcct tggcgctgat acaatgatcc
     4741 gccttgttga catcggcaaa gaccggaatc gcgcccgctt ggacgatggc gaacgatgag
     4801 gcaatgaacg tgtaggacgg aacgatcacc tcatcgccag gcccgatgcc cgctgcggcg
     4861 agggcaacgt gaagtgcact ggttccgttg gatgtgctga tgcagaactt tgccccattg
     4921 tactgagccc atttcttttc gaactccatt cccttgacgc ctgtccagta gttcactctg
     4981 ccattctcga gtggctcgag tgctgccttc ttggttgcct cggagaattg gggccagccg
     5041 gggaacggct tggtgacggt ctttttgccg ccgtgaattg caagcttctc tgccacgata
     5101 tgcctcctca ttatgcgcgc agtgagagtc tccggtcggt gcagaaggag tgtcctgcgc
     5161 gctttgtttt tttgggcgag actccagtcc ttctgacgaa gaacaataac tgtttcattc
     5221 gttcagatca acttttcata atcatgtaac ttcaaaaaga ttggggactc cgaactattc
     5281 acgctctgcg tctcgttcgg tcgttcgccg cctcagacat aacaagatca ttccataggg
     5341 cacatgttca gaaaacgaaa aagtgcatac gagtgcacaa cttcactgcc tggagagaaa
     5401 tacaaacaga gtgagtttgg cccacgcgct cgcaggtgca aacctgcccg aaagaaatcc
     5461 ccctgtgcct cttggtgaga gaagcacagt ggtaatctca gtagtgggcg gaacccacgt
     5521 gagtacttgt ctattctccg tttcggaaaa ccgcgctcac tgcagagccg gtttccgacc
     5581 agctggcgtg ggctggctca gtacgatgcc cagtcggatt ctcggacgcc actcgacgcg
     5641 tagccgactt ccaactggat attgtcaaag taggctgttc caatctgtcc ttcaggagca
     5701 ccgacgcgga tctggttgaa caaattgtcc tgaagaatgt ccgtttcaaa gatcttctgg
     5761 ttgtcagccc agaactcggt cattgcccct gtca
//
LOCUS       DAOQUC010000047         5781 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_15313_length_5781_cov_3.168530, whole genome shotgun sequence.
ACCESSION   DAOQUC010000047 DAOQUC010000000
VERSION     DAOQUC010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5781)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5781)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5781
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15313_length_5781_cov_3.168530"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2708
                     /locus_tag="PKH07_01430"
     CDS             <1..2708
                     /locus_tag="PKH07_01430"
                     /inference="COORDINATES: protein motif:HMM:NF025330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HNT33640.1"
                     /translation="ESWTELPNMLTPREDLQAVVLNGKIYVFGGNNAGNSLKSCEVFD
                     PDTNRWFRLPDFTAPRGNFCTFTDNGDIWLVGGLGLPSTYYSSCEVYDPSAGGCSGGG
                     QITQLPLTPISMLAGVPKNLILTADFNEGPRPDFVINTALFGYAGTTVGSPNEAVSDY
                     LGIAVGPEIPVSFTTPTDTPTPTPEPEQGFRLELLNPGLVVQPGEQPRLYISVVPLGG
                     FRENVDLTLTDGTVSHQLVPPLLRPPSVASLILEPVGADAVSGTRTIQVKGTSFIENP
                     IFTEFVEQLDIEYSVANPLDSQSSLFVAANRSPISLGDSTELVGRLVPALDAADIVLY
                     IELSEDGTVLRTYEVSARTNATGRFRYLFSPDEPGHYEINASWAGSEQVPPAMSEWAQ
                     LDVLPRKSRISLRVETEPPFQTGDTIHFSGRVSPSFEGLPVMAQVVVNYLSVSHTETY
                     PVSFDGEDYSGDFVLQHPGVVRMTASWTGVPQFTEGSISPPVLFLVEDTAGGTSLQTA
                     SFWDSGVSVIVAGGSSTVLSEPTRDFLTNFSYSVLRSRRYNDLDLVYVNNNAFQDYNW
                     DGQDDGIVDVTESSPKAIAEAFDTAAGLLTGSEPVSLFLLASEAQGTQLMMGDGTSLN
                     AEALHTMIETKFGTQRDIRILVDASRSGTFGEALIAPNRTVMCSATHENASYAAGGLL
                     SFAQLYWTKVQEGWGVKDSFQYARDYLNATFGYYGAPVPAMYPATAFDVDLLYYGLSS
                     DATDMLAPVIEGVVEPFVLEGTREAEIFAWVTDDVKVESVVVSIVKPGGGIFSLPLQQ
                     SSPNSRKYAVRFSEPQSATLLNELGAYLATVVAMDSSRNCSEPFSTRFAVVSASDLGG
                     DGKVDASDLLLIIRQLDHPVGLLDIYPDGRFDFKDFFLLARDWNP"
     gene            2711..3937
                     /locus_tag="PKH07_01435"
     CDS             2711..3937
                     /locus_tag="PKH07_01435"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNT33641.1"
                     /translation="MIWSSMALVVLDAVDSPDIMQTMTKDQHGVAVVGCGALAQMMHL
                     PHVVSNPRTKLVVACDLDRDTAEACRSRFGAERAETDWHQVVASRDVDLIILATHTNL
                     RAELIVPALHAGKPVYTEKPLANSIEEMHTIVRASRETGVPVCVGHNRRSSPAMLEFR
                     RLVEKALNGARPTAPTMDRSVNRAPIPEESRMQILIRINDDCRSWKDWIFHDPQGILY
                     AEMVHFIDLALWLNPSKPVKVFAEGSRCGNFAIVLRFEDGSITTMHHSLSGHFDYPKE
                     LFECTVRHFSIAMEQHIEIRQCGFDDEPALKVFPYDSECSWAVQKGVAGYFREMEAER
                     RRAKAVGGNPKWLNVNKGHYDHLDRFLDCIEGRGSNPCDAADSVPVIVVAHKLQAAID
                     EGCPLSIISDELEASL"
     gene            3993..4922
                     /locus_tag="PKH07_01440"
     CDS             3993..4922
                     /locus_tag="PKH07_01440"
                     /inference="COORDINATES: protein motif:HMM:NF013706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HNT33642.1"
                     /translation="MKLVSYRVGKEIRGGIVVGDKILDLQKVLGKREQESILCLLQGG
                     APALKAAREAVASLTHPRMLRALVGTVGSVRLEAPIPRPGKILALAGNYAEHIKEGGK
                     TFKVKDTITPRIFIKPATGVIGHEAPIPFPKAAQFIDWEGELGVVIGKSAKYVPRERA
                     MQYVAGYTVFHDVSERKLLIRKRKESSEWDKFFDWLNGKWFDGFAPMGPWLVTRDEIR
                     NPNNLNVETRINGKVEQSGNTSQMIFDVADLVHYASQIFTLEPGDVIATGTPAGVGSG
                     KGICMKPGDVAEIEIEKLGVLRNRIVREAKSRS"
     gene            4943..5503
                     /gene="yfcE"
                     /locus_tag="PKH07_01445"
     CDS             4943..5503
                     /gene="yfcE"
                     /locus_tag="PKH07_01445"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF006988.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HNT33643.1"
                     /translation="MILGVVSDTHGSLLWWRKAYEYLKDADAIIHCGDVFYHGVKNPI
                     PEGYDTLGLAQALNEIRVPLLIAKGNCDSSVDQVVLDFPMSSGMASIHTPGGVVLAQH
                     GDVADRSVQEKWVRRAHLAAFVSGHTHIPKIEKVGDAVLMNPGSVSIPLGGSFRSVGK
                     LILDCNRRCATVFNLEASCTAVEEEF"
     gene            5505..>5781
                     /locus_tag="PKH07_01450"
     CDS             5505..>5781
                     /locus_tag="PKH07_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015263589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNT33644.1"
                     /translation="MYISFHGQPTDQPVVRIGTIGCGSHSFRNIYATFQYVPVDLVAT
                     CDLLLEKAQAFADKFGAKSAYQDYREMLSKESLDAVFVVVGYDSKGRP"
BASE COUNT         1287 a         1459 c         1641 g         1394 t
ORIGIN      
        1 cggaatcatg gactgagttg cccaacatgt tgactccgag agaggatttg caggcggttg
       61 ttctgaacgg aaaaatctac gtctttgggg gaaacaatgc tggaaattcg ctgaagtctt
      121 gcgaagtctt tgacccggac accaaccgtt ggtttcgcct tcctgacttt actgcgccga
      181 ggggtaactt ctgcacattt acagataacg gagacatctg gctggtaggt ggtctgggtc
      241 ttcccagcac ctactattcc agttgtgaag tctatgatcc ttctgcgggt ggatgctcgg
      301 gcggcggaca gattacgcaa cttccactta ctcccatttc catgttagca ggtgtgccga
      361 agaaccttat cctgacagca gatttcaatg agggtcctcg tccggatttc gtgatcaaca
      421 cagctttatt tggatatgcc ggaacgacag tgggttctcc caacgaagca gtttcagact
      481 acctggggat tgcggtcggt ccggagattc ccgtttcatt cactacaccg acagacaccc
      541 ctacccccac gccagaacct gaacagggat ttcgactgga gttgctcaac ccaggtttgg
      601 ttgtccaacc tggagagcaa cccaggctct acatctccgt tgtcccgttg ggtggattcc
      661 gagagaatgt tgacctgacg cttaccgacg ggacggtctc acaccaactc gtcccacctc
      721 ttctcaggcc accgtccgtt gcatcgctga ttttggagcc tgttggtgcg gatgctgtct
      781 ccggaacacg caccatccag gtcaaaggca ccagtttcat tgagaacccc atcttcacag
      841 agttcgtgga gcaactggac attgagtact cagttgccaa cccacttgac tcacagtctt
      901 ctctgttcgt ggcagcaaat cgaagtccta tctcattggg tgattcgacc gagctggtgg
      961 gtcgcctggt acctgctctc gacgctgctg acatcgtttt gtacattgaa ctgagcgagg
     1021 acgggacagt cctgagaacg tatgaagtgt cagcaaggac caacgccaca ggcagattta
     1081 gatatctgtt cagcccggat gaaccgggtc actacgagat caatgcttcc tgggcaggga
     1141 gtgaacaagt accccctgcg atgagtgaat gggcacagtt ggatgtattg ccaagaaagt
     1201 ccagaatctc actccgcgtc gagaccgaac caccatttca aaccggtgat accatacact
     1261 tttccggacg cgtcagtccc tcgtttgagg ggcttcctgt catggcacag gttgtggtga
     1321 actacctctc tgtctcacac acggagacct accctgtcag tttcgatgga gaggattact
     1381 cgggagattt cgtcctccag cacccgggcg tcgttcgaat gactgcatcc tggacaggtg
     1441 ttcctcagtt caccgagggt tcgatcagtc caccggttct cttcctggtt gaggatacag
     1501 ctggtggcac aagccttcaa actgcttcat tctgggattc aggagtctcc gtcattgtgg
     1561 cgggtggttc cagcactgtg ctgtctgaac cgacaaggga cttcttgacc aacttctcct
     1621 actccgtgct tcgatccaga cggtacaatg accttgacct tgtctacgtc aacaacaatg
     1681 cgtttcaaga ttacaactgg gatggtcagg acgacgggat cgtggatgtt accgaatcat
     1741 cacccaaagc gattgccgag gcatttgata cagctgcagg cctgcttacc ggctcggagc
     1801 cggtatcgct gtttctgctc gcttcagagg cacagggcac ccaactgatg atgggagatg
     1861 gcacttcgct gaacgcagag gcgcttcata cgatgatcga gacaaagttc ggaacacaac
     1921 gggatattcg aatccttgtg gacgcctcac gctcggggac ttttggagaa gccttgatcg
     1981 cgcccaacag gacggtgatg tgcagcgcca ctcatgagaa cgcttcttac gcggcgggtg
     2041 gactcctctc gtttgcacag ctttattgga cgaaggttca ggaaggttgg ggcgtcaaag
     2101 acagtttcca gtacgcgcgt gactatctca acgcaacgtt tgggtactat ggagcgccgg
     2161 tgcctgccat gtatccggca acggcctttg atgtggactt gctctattac gggctttctt
     2221 ccgacgctac agacatgctg gcacccgtca tcgaaggtgt tgtcgaaccg tttgtcctgg
     2281 agggtacaag ggaggcggag atctttgcct gggtgaccga cgacgtgaag gtggagagcg
     2341 ttgtcgtctc catcgtcaaa ccagggggag gcattttcag tctgcctctg cagcagtctt
     2401 cgccgaactc cagaaagtat gctgtccggt tctctgagcc acaatcggcc accctcttga
     2461 atgagttggg tgcctacctg gcaacggtgg tcgccatgga cagttcaaga aactgctctg
     2521 aaccgttcag tactcgtttt gccgttgtca gcgcttcgga cttgggtggt gatggaaagg
     2581 tggatgcgtc cgatctgttg ctgatcatcc ggcagttgga tcatcctgtc ggactgctgg
     2641 acatctatcc ggacggtcgc ttcgacttca aggatttctt tcttcttgcc agagactgga
     2701 atccctgatc gtgatctgga gttcgatggc tcttgtggtg cttgacgccg ttgactctcc
     2761 tgatatcatg cagacgatga caaaagatca acatggtgtt gccgtggttg ggtgtggcgc
     2821 tctggcgcag atgatgcacc tgcctcatgt ggtgtcaaac cctagaacga agttggtggt
     2881 ggcctgcgat ctcgatcggg acactgccga agcgtgccgg agtcgctttg gcgctgagcg
     2941 tgccgaaaca gactggcatc aggtcgtcgc atcaagagat gttgatctga tcatccttgc
     3001 cacgcacact aatttgcggg cggaactcat tgttcctgcg cttcatgctg gcaagccggt
     3061 ctatactgag aaaccccttg cgaattcgat tgaggaaatg cacacgattg tccgtgccag
     3121 ccgggaaacc ggagtacctg tttgtgtcgg tcacaatagg cgctccagtc ctgccatgct
     3181 tgagttcaga cgtctcgtcg aaaaggcgct caatggagca cggccaacgg ctccaacaat
     3241 ggatcgaagt gtcaaccgag caccgattcc tgaagagagc cggatgcaga ttctgatccg
     3301 aatcaacgat gactgtcgaa gttggaagga ctggatattc catgatccgc aggggatact
     3361 ctacgccgag atggtgcact tcatcgatct tgcgttgtgg ctgaaccctt ccaagccggt
     3421 taaggtgttt gcggaaggtt cccgctgtgg aaactttgcc atcgtcctgc gattcgaaga
     3481 tggctccata acgactatgc accacagcct ctcaggtcat ttcgactatc cgaaggagtt
     3541 gttcgagtgc acggtgcggc atttcagtat cgctatggaa caacatattg agattcggca
     3601 atgtggcttt gacgatgaac cagcgttgaa ggttttcccc tatgattccg agtgctcttg
     3661 ggctgttcag aagggagtag cgggctactt tagggagatg gaagcagaac gacgccgagc
     3721 caaggccgtg ggaggaaacc ccaagtggct caatgtgaac aagggacact atgatcatct
     3781 cgaccgcttt ctcgactgta tcgaaggtcg aggctccaac ccatgcgacg ccgccgatag
     3841 tgtgccggta atcgttgtgg cgcacaaact tcaagcggcc attgacgaag gctgccctct
     3901 cagtatcatc tccgatgaac tggaggcttc tctctaaagg gagaggattt gagcaaatcc
     3961 gaactggact gcacattgaa aggaaagcat ccatgaaact ggtcagttac agagtgggaa
     4021 aggaaattcg tggtggcatc gttgttgggg acaagattct cgacttgcag aaagtgcttg
     4081 gcaaaagaga acaggaatcc atactgtgcc tgcttcaggg cggagcgcca gcactcaaag
     4141 cggccaggga ggccgtggcg tccctgacgc atcccaggat gttgcgtgct ttggtgggca
     4201 ctgttggatc agtcagactg gaagcgccga ttccgcgtcc aggaaagatt cttgctcttg
     4261 cgggcaacta cgcggaacac atcaaagaag ggggaaagac gttcaaggtc aaggacacta
     4321 tcacgcctcg catcttcatc aaaccggcca ccggtgtcat tggtcatgag gctccgatcc
     4381 cgttccccaa agccgcgcag ttcattgact gggaaggaga actcggagtg gtcatcggca
     4441 agtcggccaa gtacgttcct cgagaacgtg ccatgcaata tgttgcagga tatacggtat
     4501 tccatgatgt cagtgagcgg aaactgctga tccgcaagcg aaaagaatcc agcgaatggg
     4561 acaagttctt cgattggctc aatggaaaat ggttcgacgg ctttgctcca atggggccct
     4621 ggttggtcac gcgtgacgag atcagaaatc ccaacaatct gaacgttgag acccgtatca
     4681 atggcaaggt ggagcagagt ggcaatacca gccagatgat cttcgatgtc gccgatttgg
     4741 ttcactacgc ttcgcagatt ttcacactgg aacccggtga tgttattgcg acgggaacgc
     4801 cggcaggtgt aggttccggg aaaggcattt gcatgaagcc cggcgatgtt gctgagatag
     4861 agattgagaa actcggtgtt ctgcgaaaca ggatcgtgcg ggaagccaag tctcgatcct
     4921 gacgttcttg ggagagcgta cgatgattct cggtgttgtc agcgatacgc acggttcact
     4981 cctgtggtgg cgcaaggcgt atgagtatct caaggatgcg gacgccatca tccattgtgg
     5041 cgatgtcttc taccatggcg tgaagaatcc gattccggaa ggatacgaca cgctggggct
     5101 ggcacaggcg ctcaatgaga ttcgtgttcc gctcttgata gccaagggaa actgcgattc
     5161 ctctgtggat caggtggtgc tggattttcc catgagcagt ggcatggctt ccattcatac
     5221 tccaggcgga gtcgtcctgg cacagcatgg ggatgtggct gatcgcagtg ttcaagagaa
     5281 gtgggttcga cgggcacatt tggcggcatt tgtctctgga catacgcaca tcccgaagat
     5341 cgaaaaggtt ggggatgcgg tcctcatgaa tccaggcagc gtctcgatcc cgttgggagg
     5401 tagttttcga agtgtaggca agttgatcct ggattgtaat cgccgctgtg cgactgtctt
     5461 caatctggaa gcatcttgca ctgcagtgga agaggagttc taggatgtac atcagttttc
     5521 atggacaacc caccgatcag ccggtggtgc gtatcggcac gattggctgc ggttctcact
     5581 cattccgcaa catctatgcg acctttcagt atgttccagt ggatcttgtt gcgacgtgcg
     5641 atctgttgct ggagaaggct caggcgtttg ccgataagtt cggtgccaag agcgcttatc
     5701 aggattaccg ggagatgctg tccaaggaat cacttgatgc agtctttgtt gtggtgggat
     5761 acgactcaaa aggccgtccg a
//
LOCUS       DAOQUC010000048         5746 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_15410_length_5746_cov_3.031980, whole genome shotgun sequence.
ACCESSION   DAOQUC010000048 DAOQUC010000000
VERSION     DAOQUC010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5746
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15410_length_5746_cov_3.031980"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..500
                     /locus_tag="PKH07_01455"
     CDS             <1..500
                     /locus_tag="PKH07_01455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33645.1"
                     /translation="SSTALNNVVQGWINGSYANRGLLFRNSTANEDDDGAHKFYSAEQ
                     GSAPPSLYIRYALPVVATSTPSNTPTPTVTNTPTRTPTATSTYTFTNTPTATATFTFT
                     HTPTRTPTATATHTPTLTWTPSQTPTGTFFPTATLTPTPVFTPTATAVQTQTTGQKAR
                     LWEGY"
     gene            776..1372
                     /locus_tag="PKH07_01460"
     CDS             776..1372
                     /locus_tag="PKH07_01460"
                     /inference="COORDINATES: protein motif:HMM:NF014669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HNT33646.1"
                     /translation="MRFNVRKDAVAIARIREFDFQDALFAAQSVLAFTGLMILGAKMR
                     VLLPFSPVPFTMQVFFALLAGVLLGPGMAAASQALYLALGAAGIPVFSGSVAGLAYLM
                     GPTGGYLISFLFVPQIVARILGRGVTINGKTEINRVRMILALAVGVAVIHLMGILHLC
                     TLTGGRLMTAFIMGLPFIPADVVKAIAVAWITPRLMKR"
     gene            complement(1515..2597)
                     /locus_tag="PKH07_01465"
     CDS             complement(1515..2597)
                     /locus_tag="PKH07_01465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33647.1"
                     /translation="MPTLLFYVSGHGFGHSTRTIAVINAIPPEYDVIVKTSAPQWLFD
                     LNVRRAFDFQPLEVDVGAVQPNCLKLEREKTLLAFSQFVAGQDVIVRNEVQFARPKDI
                     RAVVFDIPPLAPLVAQALGVPSIAISNFSWDEIYEPYVREFPQYQPLLAKLAAEYATT
                     SLLLRLPFHLPMAAFPVREDWHLLTRVAQEDKAKLRAKMGLPRDEIVVLLSFGGFRLA
                     KTDALDLASCKGFFFLSFVQPPLTEAANYRNLRQDECRHEEAVKASDIVITKPGYGIV
                     SECIANQTAMLYTEREDFCEYPLLVEGIQKHLIQDHIPQDDLLVGRWHSHLERLSVRL
                     DSWNTVEPVAIDDGSRTAARIIEMAR"
     gene            complement(2597..2851)
                     /locus_tag="PKH07_01470"
     CDS             complement(2597..2851)
                     /locus_tag="PKH07_01470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33648.1"
                     /translation="MGQKSPISGEVPGKLNSLHGTLQTAGILALEDLKKGLKSGRLNR
                     MRETGTARLAIQSAESCKTTSCMALTAGTVIKQLATPGLS"
     gene            3011..>5746
                     /locus_tag="PKH07_01475"
     CDS             3011..>5746
                     /locus_tag="PKH07_01475"
                     /inference="COORDINATES: protein motif:HMM:NF039776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdrD B-like domain-containing protein"
                     /protein_id="HNT33649.1"
                     /translation="MFACRNLCKADPFRLSTITRFSVFLLVALVPLFPNQAAFSQDGF
                     ELTWDNVAKNPKDKTAPERLMNPPIPFGGEFQANSISWEDQYDPAAAMAGDGSFVVVW
                     DTDYYGSFPYFVQTVMGRRFLPGATPATVDFPISPLLTPTPFESLRAEEPDVAINEAG
                     EFVVVWANLVSLDSKIMGSIFDASGIPQVISSPLSTMTMYEVHEPAVDLNETGTFVVV
                     WEDTFSSTASIKGRTFNLQATPLTDETTLGQEGVSSLFWNDHPDVALHDDGHFVVVWG
                     SQISMPGYSYCDAIVGQVLNPEMTPISGEHILSASVCQSQLPSVGMDADGDFVVAWEI
                     YGEPSYVYGRVFNAMATPVTDPFFIGEGRRPAVAVHPDGDFVVSWHYVSYGPQNTGTR
                     IGALPSEPDAIFVQAFNAQSTPITTPAPANSSTLFSLGRPCIGMNQAGSFVVCWESYM
                     QDSFGNGVFGQLYDIPTSTATDTATVTPTDTATDTPSDTPTNTSTETPTETPTETPTN
                     TPTATDTPTDTPTHTPTDTATDTATDTPTETPTATPTETPTSTPTPYALIYGFAFDDA
                     NENGMMDFGEDVLEGVNIALYRDSALEQTRTTTATGYYEFEILSLGTYEVEETDPVGY
                     KSTTANQVTVVVSSYASYRVDFGDTPILYDFGDAPDPTYPTLLSSDGARSEIGFLYLG
                     YGVDSESDGLPSINADGDDLNGSDDEDGVQFLTPLVAGEQMTMSIISSASGYLNVWFD
                     LNGNGAWDHATEQIVIDRVIVPLVSDIQFSVPSDIYVSGAVYARFRLTLAPGAQPYGL
                     HSMGEVEDHALYFTLPTSTPTETPTDTATETPTDTPTDTPTDTPTDTPIPETPTETPT
                     ETPTDTPTDTPTETPSNTPTDTPTQTPTETPSDTPTNSPTRTPTRTPTYTSTVTPE"
BASE COUNT         1349 a         1575 c         1493 g         1329 t
ORIGIN      
        1 ggtcttccac ggcgctcaac aatgtggtgc agggatggat caatggcagc tatgccaatc
       61 gaggtctgct gttccgcaac agcacggcca atgaggacga tgacggcgca cataaattct
      121 actcagcgga gcagggatcg gcaccacctt ctctctacat tcgctacgca ctgccggttg
      181 tcgcaaccag tacgccaagc aatactccga cgccaacagt aaccaacact ccgacccgaa
      241 cacccacggc gacctcaacc tacactttta caaacacgcc gacagcaacg gcaactttca
      301 catttaccca tacgccgaca agaacgccaa cggcaacggc gactcatacg cctacgttga
      361 cgtggacgcc gagccagact ccgacgggaa cattctttcc aacggcaaca ctgacaccga
      421 ctccggtctt cacgccgacc gcaactgcgg tgcagaccca gacaacggga cagaaagcac
      481 gactgtggga ggggtactaa acggatcggg ttttccgctt ctgggaaagc agacttcgag
      541 caggtgagca gttgagggtg cgtgaacttt gtccactccc tcgcacgctc gctcggcaaa
      601 tgacacgcct gttctgaact gagtgtcttt gggagcaggg cagtgtttgc aggtaccaac
      661 ctgcttgaaa agaatcctgg ggaatgatat cttcctttcg gcttgagaac gagacaggtt
      721 ttcaatgcct atcacgttgg ggactctccc aaccatagga tggagtcgca ggaaaatgag
      781 attcaacgtc cgaaaagatg ctgtagcgat agcaagaata agggaattcg actttcagga
      841 tgcactcttt gccgcgcagt cggtattggc tttcaccgga ttgatgatcc tgggcgcgaa
      901 gatgagagtc ctgctgccgt tctcgccggt gccgttcacg atgcaggtct tttttgcgct
      961 cttggcgggc gtgttgcttg gcccaggaat ggctgctgcc agccaggctc tctatctggc
     1021 tttgggtgcg gcaggcattc cggtcttcag cggtagcgtg gcgggacttg cctatttgat
     1081 gggacccaca ggcggatatc tcatcagttt cctttttgtg ccgcagattg tcgcccgaat
     1141 cctggggcgc ggagtcacca tcaacggcaa gaccgaaatc aaccgcgttc ggatgattct
     1201 tgctcttgct gttggagttg ccgtcattca cctgatgggc atccttcacc tttgcacact
     1261 cacgggcgga cgcctcatga ccgccttcat tatgggtctg cctttcattc ccgcagatgt
     1321 ggtgaaagcc atcgccgtgg cttggatcac cccgagattg atgaagagat aagtctgaac
     1381 cgctgattac gcagattaaa ggatgacaca gatttcagaa atcctttcac gtcggggatg
     1441 tttcttcttg ttctgcaaac ctgtgaaatc cgcgtcatcc attgaatccg tggttcagac
     1501 attgctgcgg cctgtcatcg tgccatctcg atgattctcg ccgccgttct gctgccgtcg
     1561 tcaatggcga caggttctac ggtgttccag gaatcaagac ggactgagag gcgttccaag
     1621 tgagagtgcc atcgtcccac cagcagatca tcctgtggaa tgtgatcctg gatgaggtgt
     1681 ttctggatgc cttcgaccag cagcgggtat tcgcaaaagt cttcccgttc tgtgtagagc
     1741 attgccgtct gattggcgat gcattcggag acgatcccat agcccggttt ggtgatgacg
     1801 atgtcagagg ccttgactgc ctcttcatga cggcattcat cctgtcgaag gtttctgtag
     1861 ttggcagcct ctgtcaatgg tggctgaaca aacgacagga aaaagaagcc tttgcaggaa
     1921 gccagatcga gcgcatctgt cttcgcgagc cgaaagcctc caaacgaaag aagaacaacg
     1981 atctcgtccc gcggaagccc catctttgcc cgaagtttcg ccttatcctc ttgcgccacc
     2041 cgagtcagca gatgccagtc ctctcgaacg gggaaggcag ccatcggcag atgaaagggc
     2101 agacgcagca gcagactcgt tgttgcgtat tcggcagcaa gtttcgcaag aagcggttgg
     2161 tactgcggaa attcacggac gtatggctca tagatctcat cccaggagaa gttactgatg
     2221 gcgatgctcg gaacacccag cgcttgggca accaatggag caagtggcgg gatatcaaat
     2281 acaacagcgc ggatgtcttt cggacgagca aactggactt cgttgcgaac gatgacatcc
     2341 tgacctgcaa caaactgaga gaacgcaagg agtgtcttct ctcgttccag tttgagacag
     2401 tttggttgta ctgcaccaac atccacttca agtggctgaa agtcgaacgc acgacgaaca
     2461 ttcagatcga acaaccactg tggcgctgat gtcttgacaa tcacatcgta ttcgggcggg
     2521 atggcgttga tgacagcaat ggttcttgtg ctgtgaccga aaccgtgccc actgacgtaa
     2581 aacagaagag ttggcatcaa gatagtcctg gtgtggcaag ttgtttgatg acagttcctg
     2641 cggtcaatgc catgcaggaa gtggttttgc aggactcagc ggactggatt gccagccgag
     2701 cagttcctgt ttccctcatt ctgttcagtc ttcctgactt cagtcccttt ttcaggtctt
     2761 ccaaagctag aatccctgca gtttggagcg ttccgtgaag actattgagt ttgcccggta
     2821 cttcaccaga gattggcgat ttttgtccca tcctcgttct ccgtcagctt accgaaaaac
     2881 gcttgttctc gtcttcaaag aagtgtatca tatcctcgat aagtcgaaag acaatagttt
     2941 ccttgagagc agttcgagaa cgagcgagaa gtctaaggga cagtgtcgtt tttccaggga
     3001 ggatatatcc atgtttgcct gccgtaatct ctgtaaggcc gatccgtttc gtctgagtac
     3061 gatcacacgg ttcagcgtct ttctgcttgt ggcgttggtg ccgctgtttc caaatcaggc
     3121 ggccttcagc caggacggct ttgaactgac ttgggacaat gtcgccaaga atccaaagga
     3181 caagaccgcg cccgagcgtc tgatgaatcc tccgattccc ttcggtggtg aatttcaagc
     3241 caactccatc tcatgggaag atcaatatga tcctgccgct gccatggctg gcgatggaag
     3301 tttcgttgtg gtctgggaca cagattacta tggcagcttt ccgtacttcg tccagacggt
     3361 tatggggcga aggttcctgc caggagcgac acccgcgact gttgacttcc ccatttctcc
     3421 gctgttgact cccactccat tcgagtcgct ccgtgcagaa gaaccggacg ttgcgatcaa
     3481 tgaagcgggt gaatttgtcg tcgtctgggc aaatctcgtc agcttggaca gtaaaatcat
     3541 gggatccatc tttgacgcct cgggtattcc gcaggttatc agtagtccgt tgtccacaat
     3601 gacaatgtac gaggtgcatg agccggcagt agacttgaac gagacaggaa catttgtcgt
     3661 cgtctgggaa gacaccttca gttccacagc ttcgatcaag ggacgaacct tcaacctgca
     3721 ggcaactccg ttgacggatg agacgacgct cggccaggaa ggtgtctcaa gtcttttctg
     3781 gaacgaccac ccagacgttg cccttcatga tgacggtcac tttgttgtcg tttggggttc
     3841 tcagatcagc atgcccggtt attcctactg cgatgccatt gttgggcagg tgctcaatcc
     3901 ggagatgact cccatctccg gcgaacatat tctctccgcg agtgtctgcc agagccagtt
     3961 gccttcagtt ggaatggatg cggatgggga ttttgtggtg gcctgggaga tctacggtga
     4021 accgagttac gtctatggcc gcgtcttcaa tgccatggca acaccggtaa ctgatccctt
     4081 tttcattggt gagggacgga gaccagcggt tgcagttcac cccgacggag actttgtggt
     4141 gtcttggcat tatgtgagct atggacctca gaataccggg acaagaattg gtgcacttcc
     4201 atcagaacct gacgccatct tcgttcaagc attcaacgcg cagtctacac ccatcaccac
     4261 accggcgccc gccaattcat ctaccttgtt ctccctaggg cgtccctgta tcggaatgaa
     4321 tcaggcaggg agttttgtcg tgtgttggga atcttacatg caggacagct tcggaaatgg
     4381 tgttttcggt cagttgtatg atattcccac cagcacagca accgatacgg cgacggtgac
     4441 tccaactgat acggcaaccg atactccttc ggacacgcca acaaatacct caacggagac
     4501 acccacagaa actccaacag agacaccaac caataccccg accgcgacag atactccgac
     4561 ggataccccg actcataccc cgacagatac ggcaactgac acagcgaccg acactccaac
     4621 cgaaacaccc acggccaccc cgaccgagac tcccacatcc acacccactc cctatgcact
     4681 catctatgga tttgcgttcg atgatgccaa tgagaatgga atgatggact ttggggaaga
     4741 tgtgctggaa ggtgtgaata tcgctctcta tcgggattca gcattggagc aaaccaggac
     4801 gaccacggca acgggatact atgagttcga gattctgtct ctgggcacct atgaggttga
     4861 ggagacagat ccagtcgggt acaagtctac tacggccaat caggttactg tcgtggtttc
     4921 atcttatgcc agttacagag ttgattttgg agacacgccc atcctctatg atttcgggga
     4981 tgcacccgat ccgacctacc ccacactgtt atccagcgat ggtgctcgca gtgaaatcgg
     5041 ttttctctat cttggatatg gtgttgactc agaatccgac ggacttcctt ccatcaatgc
     5101 cgacggagac gatctgaacg gcagcgacga cgaagacgga gttcagttcc tcacgccact
     5161 ggtggcggga gaacagatga ccatgtcgat catatcgtca gcgtctggat acttgaatgt
     5221 ctggtttgat ctcaatggca atggcgcctg ggatcatgca accgagcaga tcgtcataga
     5281 tcgggtcatc gttccactcg tgagcgatat ccagttttct gtaccatcgg acatctatgt
     5341 gtccggtgca gtgtatgctc ggttccggct gacattggct ccaggggcac agccctatgg
     5401 gttgcacagc atgggtgaag tggaagacca cgctctgtat tttactcttc ccacaagtac
     5461 tccgacagag accccaacag acacggcaac cgagacccca acggacactc cgacagatac
     5521 gccgaccgac accccaacag acactccgat tccagaaacg ccaactgaga ctccgacgga
     5581 gacacctact gacacaccaa ccgatacccc gacagagacc ccatcgaata caccgacgga
     5641 tacgcctaca cagactccga cggagacccc atcggatacg ccgacgaaca gcccgactcg
     5701 gacaccaacg agaacaccaa cttatacttc aacggtcacg cccgag
//
LOCUS       DAOQUC010000049         5706 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_15539_length_5706_cov_3.499558, whole genome shotgun sequence.
ACCESSION   DAOQUC010000049 DAOQUC010000000
VERSION     DAOQUC010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5706
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15539_length_5706_cov_3.499558"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1472)
                     /gene="selD"
                     /locus_tag="PKH07_01480"
     CDS             complement(<1..1472)
                     /gene="selD"
                     /locus_tag="PKH07_01480"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: protein motif:HMM:NF002098.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HNT33650.1"
                     /translation="MRFPEKRRLIMKRSMALGHCVCNPRKPCPCDTLIQHDLCPCAGE
                     RPDPPKGTVKLTRFVRKAGCASKISRADLKRVLKDLPIYEDPDVLIGIPAGDDAGVYR
                     IEGGHNLVQTVDVFSPVVDDPFLFGQISAANSVSDIYAMGGRPICALSIIGFPIEELP
                     HEIMREILRGGIEKMREASVSVIGGHSINDEEIKCGFAVTGLVKGTGSITNSDAREGD
                     WIVLTKPIGTGLISFAAQIGLASDEATALIGQSMAELNRDAAELMIRFHADACTDVTG
                     FSLIGHLSEMAFSSGLTAQVQLALVPAFAESLGCLRSEIIPGATERNKESFSEWVQVL
                     GTGEPVLVDLLYDAQTSGGLLVALPPDRAPRYIEEMQTRGHCFTSKIGEFLKSEDCRV
                     RVVLGEPTNLAGICQLRERPQPVAEQSCCAEEFSCCAEPEVRPVERRVENVEVPMSGQ
                     EKKTSESFKEFMKSVNAPGSVDARTKRLLAIALSISERCGP"
     gene            complement(1469..2842)
                     /gene="selA"
                     /locus_tag="PKH07_01485"
     CDS             complement(1469..2842)
                     /gene="selA"
                     /locus_tag="PKH07_01485"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNASec selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HNT33651.1"
                     /translation="MSDSRRFLPSVDKILLRLQSFESSEAIPHCRMVETTRAVLDEYR
                     APLRGKDAPSLDEVAQQVWEMLQQEARQKLHKVVNATGIVLHTGLGRAVLPREAMDAV
                     ADLDGCCNVQIDLETGQRGRRDAAIEELLKTLTGAEAATVVNNNAGATLLVLAALCRH
                     REVVVSRGQLIEIGGSFRLPDVIRESGAKMIEVGTTNKTHLRDYASAITDQTAVLLHV
                     NPSNYRIVGFAQQVSTADLVSLKETYPDLLVVDDLGCGALINLEQYGLPHEATVQESL
                     KAGADVALFSGDKLIGGAQSGIIVGSKKCIDLIRKHPLARALRVGKFTIAALEATLRL
                     FLDPTTLLDRNPTLRMLTISLERLQERASQLAKRLAPLLSATVVKGFALCGGGALPTV
                     ALDTFLVAIHKEGMSADELARKLRMGEPCVVTRVESDQVLIDTRTLLDGEDELVETAV
                     RNLGTTR"
     gene            complement(2835..3689)
                     /locus_tag="PKH07_01490"
     CDS             complement(2835..3689)
                     /locus_tag="PKH07_01490"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNT33652.1"
                     /translation="MYRRRLSQLFFAVFLNFPLLGQWKSLCVPILNCHSCPWAAFACP
                     VGVIGHLVSWSVFPFLALGTILLLGALFGRLLCGWVCPFGFLQDLLHKVPIRKIHLPR
                     RLSLLKYVVLVVMVVVVPLLIGVESNAFFCSLCPAATIQAVVPWGLMGGKNLIEIVAG
                     TWIRFVVLAIIVLLAMTSRRSFCRFLCPIGAFLALCNRFSVFSLRYAQNDCPRCKRCL
                     KDCPMDVQIEDFERKGQTTVVTAPSECILCLNCTKNCHKSSLRWTLWNLFPKVKDHAS
                     VKESENHV"
     gene            complement(3778..4032)
                     /locus_tag="PKH07_01495"
     CDS             complement(3778..4032)
                     /locus_tag="PKH07_01495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33653.1"
                     /translation="MRRLFVGVCVICLSLAAIGYSFSRQFEIYEEDLTQGEFAFFEGV
                     SERILVSDATFGGVFISEKTGRFISTYDRSQPRGREACPT"
     gene            complement(4243..>5706)
                     /locus_tag="PKH07_01500"
     CDS             complement(4243..>5706)
                     /locus_tag="PKH07_01500"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNT33654.1"
                     /translation="EMKRSRGLVFWWMVLSLLVCLAVGTYTRLSAFAQIDQELREYDV
                     LLDVLNTVSRDYVDPEQARKDKLLQGAIQGMMNTLDPYSVFLPPKDKEDLDIQTKGKF
                     MGVGIRITILNGWLTVESPIPNSPAFRMGILSGDKIIRINGKSTRGLTAEDAVKVLRG
                     PKGTRVKVTILRKDAPSPMDFSITRDEIRIPTLEHSMMPAGIGYIRLYEFREDASSLI
                     DAAVQDLESQGMKAVVLDLRFNTGGLLDEAVKVAGKFLDEGLTIVVTRTRNPQDETVY
                     RNAVTPIQPYPMVLLVNQVSASASEIVAGALQDHGRAVIVGPAGSHTYGKGSVQSIIE
                     MKFGNALKLTTAKYFTPNNRSIADEGIIPDIGVDITEEQQRLVMTKDLIGKIPESATA
                     ELSGLAFGGKKDSLEALESGMPSDEEKEKRLQQMLENSESEAPTESEDTDVITAEEVF
                     VEKEQTPADKSNVTYDLELMEALRILRAGMLFSRQEG"
BASE COUNT         1336 a         1626 c         1442 g         1302 t
ORIGIN      
        1 gggccacaac gttctgagat ggacagggca atggcaagca aacgcttcgt tcgagcgtca
       61 acgctgcctg gagcgttcac agatttcatg aactctttga aggactcgga tgtcttcttc
      121 tcctgaccgg acatgggtac ctccacattc tccactcttc tctcaactgg ccgcacttct
      181 ggctcggcac agcaggaaaa ttcctctgca cagcaacttt gttcagccac cggttgcggg
      241 cgctccctca actgacagat tcctgccaga ttcgttggct cgcccaaaac aacgcgtaca
      301 cgacaatcct ctgacttgag gaattcaccg atcttacttg tgaagcaatg tccgcgagtt
      361 tgcatctctt caatgtaccg tggcgctctg tccggcggaa gggcaaccag gagtcctcct
      421 gaagtctggg catcgtacag caagtccaca agcacgggtt ctcccgttcc gagcacctga
      481 acccactcgg agaaggactc cttgtttcgt tccgttgcgc ctgggatgat ctccgagcga
      541 agacatccca aagattctgc aaacgccggc accaaagcca gttgaacctg tgcagtcagc
      601 ccgctggaga aagccatttc ggagagatgt ccaatgagac tgaagcccgt aacatcggtg
      661 caggcatcag cgtgaaatcg aatcatcagc tcggcagcat ctctgtttaa ctcagccatg
      721 gactgaccga tcagagcggt cgcctcgtcg gacgccaaac caatttgagc agcaaatgag
      781 atgagtcctg ttccgatggg tttggtcagt acgatccagt cgccttcgcg agcgtcgctg
      841 ttggtgatgc tgcccgttcc tttgaccaat cccgtcacgg caaagccgca tttgatctcc
      901 tcatcgttga tgctgtgtcc gccgataaca ctcaccgacg cttcgcgcat cttctcgatc
      961 ccaccgcgaa gaatctccct catgatctca tgtgggagtt cctcgatcgg gaacccgatg
     1021 atggacaatg cacagatggg acgtcccccc atggcgtaga tgtcactgac ggagttggca
     1081 gcgctgatct gaccgaagag aaatggatca tccacaacgg gcgagaaaac atctacggtt
     1141 tggacgagat tgtgaccgcc ctcgattcga tagacgccag cgtcatctcc cgcggggatg
     1201 ccgatcagaa catctgggtc ttcatagatt ggaagatctt tcagaacgcg cttgagatcg
     1261 gctcgactta tcttcgacgc acatcctgct ttgcgcacaa atcgggtgag tttcactgtt
     1321 cccttcggtg ggtcgggtct ttcacccgca cagggacaca ggtcatgctg aatgagtgtg
     1381 tcgcaaggac agggcttcct gggattgcac acgcagtgcc ccagtgccat ggagcgcttc
     1441 ataatgagtc tgcgtttttc aggaaatctc atcttgtagt ccccagattt ctgaccgctg
     1501 tttccaccaa ttcatcttcc ccatcaagta gcgttcgtgt gtctatgaga acttgatcgg
     1561 actctacccg tgtgacgacg cagggctctc ccatacggag tttgcgcgcc agttcatcgg
     1621 cgctcatgcc ttccttgtga atcgcgacca ggaaagtgtc gagcgcaacg gtcggcagcg
     1681 cacccccacc gcacagggca aaacctttga cgactgttgc agagagaagc ggcgccaatc
     1741 gtttcgccag ttgtgatgcc ctctcctgaa gtctctccag ggaaatggtc agcatccgta
     1801 gtgtcggatt tcgatccaaa agtgtggtcg gatcaagaaa cagcctgagt gtcgcttcca
     1861 acgcggcgat ggtgaacttc cccacacgaa gtgcacgcgc aagaggatgc ttccgaatga
     1921 gatcaatgca cttttttgaa ccgacgatga ttccagactg agcgcctccg atgagtttat
     1981 caccgctaaa cagtgcgaca tccgctcccg ccttgaggct ttcctgaacg gttgcctcat
     2041 ggggtagtcc atactgttcg aggtttatca aggcaccaca tccgagatcg tctacaacca
     2101 gcagatctgg atatgtttcc ttcagactga ccagatccgc tgtcgaaacc tgctgtgcaa
     2161 agcccacaat gcggtagttg ctggggttga catgaagcag cacggccgtc tgatcggtaa
     2221 tcgctgaggc atagtctcga agatgtgttt tgttggtagt gcccacctcg atcatcttcg
     2281 cgccgctttc gcggatgaca tcagggagtc tgaaagaacc accaatctcg atcagctgcc
     2341 cacgggaaac gactacttct cgatgacggc acaacgcagc gagcaccaaa agagttgcac
     2401 cggcgttgtt gttcaccacg gttgctgcct ctgcgccagt gagcgtcttg agcaactcct
     2461 caatggctgc atcccgacgc cctctctggc ctgtctcaag atcaatctga acattgcagc
     2521 acccatccag atcggcgaca gcgtccatcg cctctcgtgg aagaaccgct ctgccaagac
     2581 cggtatgcag cacgattccc gttgcgttca cgactttgtg cagtttctga cgtgcctcct
     2641 gttgcagcat ttcccaaacc tgctgtgcaa cctcatccag cgatggtgcg tctttccccc
     2701 gtagcggggc tctgtactcg tccaagacag cgcgagtcgt ttcaaccata cgacaatgcg
     2761 gaatggcttc agatgactcg aaagactgaa gccgaagaag aatcttgtcc acggaaggca
     2821 gaaatcgtct tgaatcagac atggttttcg ctctccttga cagatgcgtg atccttcacc
     2881 ttcgggaaga gattccagag agtccatcgg agactggatt tgtgacagtt ctttgtgcag
     2941 ttaagacaga ggatacactc tgatggtgcc gtgaccaccg tggtctgtcc ttttctctca
     3001 aagtcttcaa tctgcacatc catcggacag tccttgaggc agcgtttgca gcgcgggcaa
     3061 tcattctgag cgtaacgaag actaaagact gaaaaacgat tgcacaatgc aaggaacgct
     3121 ccgatcggac acaggaagcg gcaaaagctc cgacgcgatg tcattgccaa gagcactatg
     3181 atcgccagca ccacaaaacg tatccaggtt ccagcaacga tctcgatgag gttcttgcca
     3241 cccatcagtc cccaaggcac cacggcctgg atcgttgccg cgggacaaag ggaacaaaag
     3301 aaagcgttgc tctcgacgcc gatcagaagt ggaacgacaa cgaccatgac gacaagtacc
     3361 acatatttga gcaggctcag tcttcggggc agatgaatct tccgaatggg gaccttgtgc
     3421 agcagatcct gaagaaaccc aaaaggacaa acccatccgc acagcaggcg accgaacaga
     3481 gcacccagaa ggagaatcgt ccccagggcg agaaaaggaa acacactcca actgaccaga
     3541 tgcccgatga caccaacggg gcaggcaaac gccgcccaag gacacgaatg acagttcaga
     3601 attggaacac aaagactctt ccattgcccc aacagtggaa agttaaggaa gactgcaaag
     3661 aacaactggg agagccttct gcgatacacc ttgtcgcgat gctcctttca aaatgtggag
     3721 atcaaggaat ccgattctta ctatggagac tcccatggtc tcagtcagct actttgctca
     3781 agtcgggcat gcctcgcgac cacgaggctg actgcgatcg taggtgctga tgaagcgtcc
     3841 cgtcttctcg gaaatgaaga cgcctccaaa cgttgcatct gagaccagaa ttcgttcgga
     3901 aacgccttca aagaaggcaa actccccttg ggtgagatct tcttcgtata tctcgaactg
     3961 cctgctgaag gaatagccta ttgcggccaa gctcaggcag atgacacaca caccaacaaa
     4021 cagacgcctc atgggcgatt ctcctcttca atgggaggtt tgattgtgga agtgcggagt
     4081 gtccctgagc agcttcccca ctcagccgga tctctgtctg aaaactccat cccgctcttc
     4141 cctatccgct tctcgcctgg cgaaaagagt ttagactgtc cgccgccgtc agtatgtccg
     4201 ccgcctcgac gggtgcatcg cactctgact tgctaccggc tatcagcctt cctgtctgga
     4261 gaagagcatt cctgctcgca gaatgcgaag agcttccatc aattccaaat cgtaagtcac
     4321 gttggatttg tcggcagggg tctgctcttt ttcaacaaag acttcttccg cggtgatcac
     4381 gtctgtgtct tcgctttctg tcggtgcttc tgactccgaa ttctccagca tctgttgcag
     4441 gcgtttctcc ttttcctcgt ccgaaggcat accggattca agcgcttcga gcgagtcttt
     4501 cttgccgccg aaagcgagac cgcttaactc tgccgttgcc gactcaggaa tcttaccgat
     4561 aagatccttg gtcatcacca gacgctgttg ctcttcagtt atatccacgc caatatccgg
     4621 aatgattccc tcatcggcga tggaacgatt gttgggagta aagtacttcg ccgtggtcag
     4681 tttgagtgca ttgccgaact tcatctcgat aatgctttgg acagaacctt tgccgtaggt
     4741 atgggatccg gcagggccga caataacagc gcgaccgtga tcctgaagcg ctccggcaac
     4801 aatttcagag gcacttgccg aaacctggtt gaccagcaga accatgggat agggctggat
     4861 cggagtgacc gcattacggt agaccgtctc atcctgcggg ttgcgcgttc gagtcacaac
     4921 gatggtcagc ccttcgtcga gaaacttacc agccacctta actgcctcgt ccagcaggcc
     4981 tccggtgtta aatcgcagat cgagaacaac cgctttcatg ccttggctct ccaagtcctg
     5041 cactgcggcg tcaatcaggc ttgaagcgtc ttcgcgaaat tcataaaggc gaatataacc
     5101 gatgcctgca ggcatcatac tgtgttccag cgtaggaatt cgaatttcat ctcgggtgat
     5161 ggaaaaatcc atcgggctgg gggcgtcttt gcgaagaatc gttactttca cacgagtccc
     5221 cttgggacca cgcaggactt tgaccgcgtc ttcagcggtc aggcctcggg tgctcttgcc
     5281 attgatgcgg atgatcttgt ccccgcttaa gatgcccatt cgaaacgccg ggctgttcgg
     5341 aatcggagac tcgacagtca gccatccatt cagaatggtg atgcgaatgc cgacgcccat
     5401 gaatttgccc ttggtctgga tatcaaggtc ttccttatct tttggaggca gaaacacgct
     5461 gtagggatca agcgtgttca tcatgccctg aatagcgcct tgaagcagct tgtccttgcg
     5521 cgcctgctcc ggatccacgt agtctctgga gactgtattc agaacgtcca gaagcacgtc
     5581 atactctcga agctcctggt caatttgggc aaatgcggac agccgtgtgt acgttcccac
     5641 cgcaagacag accagaagac tcaaaaccat ccaccagaaa accagaccgc gactacgctt
     5701 catctc
//
LOCUS       DAOQUC010000050         5639 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_15768_length_5639_cov_3.844198, whole genome shotgun sequence.
ACCESSION   DAOQUC010000050 DAOQUC010000000
VERSION     DAOQUC010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5639
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15768_length_5639_cov_3.844198"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..780)
                     /locus_tag="PKH07_01505"
     CDS             complement(<1..780)
                     /locus_tag="PKH07_01505"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HNT33655.1"
                     /translation="MKTSRLPFHRVVVISNFFVSAIFCLLAFGCTTRGELMNLQDKGF
                     GSISNRSLAYTGNLNVATIQHSSIGRQPDANFIEQYLSLEELLKMRQHQLKGFEDYLC
                     WGTLESKPGVWNWEPHDRVRKHLERAGIEYVIYPWIHFPPTWYAEKHPEELMRCLEHD
                     EPTRVLSFFSPRTLDVYREFYRRVHEHFGDQVAIIYACLNGPYGEGNFPLPHVDWVVD
                     QQHSHEGWWLSDPYARSDFQRFARKRYPSLDLLNRSWATSFS"
     gene            complement(877..1506)
                     /locus_tag="PKH07_01510"
     CDS             complement(877..1506)
                     /locus_tag="PKH07_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020249000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNT33656.1"
                     /translation="MSSDPGFTLIELLIVVAIIGILAAIAIPNFIQAQIRAKVARVYS
                     DHRALDLAATAYSVDQNGFPPDRHYSAFQGGFPSSWFVADLCQLSSPIAYVKNTGVYI
                     DPFNDQLAVSGETPAIPSSYKYYNGGYGITPGCWGDVIAAVSDRLPTTACMFGTYGPD
                     HVYDGGEWVLALPSQLFRIYDPTNGVTSRGDILRFVGNTKGLPSIIGGR"
     gene            1915..2823
                     /locus_tag="PKH07_01515"
     CDS             1915..2823
                     /locus_tag="PKH07_01515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33657.1"
                     /translation="MRETLTIVVAVLLASISVQICLADDTQQKSSAQVISATKPSLIP
                     PPRREIPGRRLEIAPKQEIYVPEFFSQPADATEFALVVFFHGASWCAEQNFYDARKNA
                     VLLTVNQTDPLPFSVMGQALPEILERTEQRLLDEGLVSGRISALCITSFSGGYVAVRE
                     ILKVPEIVNRVTDVVLADSLYAPRIPEATDQLEPSALEPFLNYARQASTGKGHFWFSH
                     LFPPEEQHRGNTTTLAARYLLDKLQVQMRSASDKNSRGASLLYRADKGNFHVLGYAGM
                     TTQDHFEHFYALSDLLRLTSIPDATQ"
     gene            complement(2884..5322)
                     /locus_tag="PKH07_01520"
     CDS             complement(2884..5322)
                     /locus_tag="PKH07_01520"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNT33658.1"
                     /translation="MSKARVKRRRRSTQISQETSRSNPFSSARFLSRVPWIPVLKTVV
                     LVSVVLVSYIPAMRADFNWDDGDFLIYNDLIKAPDGLYRFWFTTEPPDYFPLTSSMLW
                     VEWRLWGMNPMGYHVVNVLLHLGTCLVLWRVLRHLAIPGAWLATLVYSVHPVNVESVA
                     WITERKNTLPMFLAMISVFLFLRAHANKDAFSRRHYLLALLLFLLSLLSKTSVAMFPI
                     FLLGCLWWINRRLTRKDLLRTLPFFALSFVLSLVTIWFQSNRAIGDDIIRTDGFASRL
                     AGAGWAVWFYLYKALLPVNLCFVYPRWDINARNPIVYIPGALLVAMGIVFWRRRDGWG
                     RPFLFAMGYYAVTLFPVLGFFNIYFMRYSLVADHWQYTSLIGVVALAVGGAVHLVQKQ
                     MPVFRQFAGAGAAVLVACFALLTWKQTHIYHNEETLWRDTIAKNPKAVIAYDNLGVRL
                     AYQGQLDKAIELHQQALRLDPDNLHAMNNLGVALARLRRFDEADRYFQRVLSLDPKFA
                     SAYNNIGLSYALQGNDESAFPYYQKALELESNHADAHNNIGNVYARKGEHDQAIEHFQ
                     KALEIHPDHPEVHYDLAVSLSFQGKFEQAKQHYLKAIEAKPFFPDAHNNLGALYQQQG
                     DIENAIARFQMALRWNPSFTSARLNLANALALAGKFHEALPNYEHLMKAEPEDAEIRS
                     RYGDALAHIGRVREAMELYREALRLQPDWAMLEAKLAMMLAVNDDPALKNVAEAVRLG
                     ENARRKEGEDNPVIMDTLGMIYAEAGRFTEAAACAEKALSIARGHGDDLFAQDIEERL
                     NLFRSGKTFRFVSE"
BASE COUNT         1355 a         1488 c         1508 g         1288 t
ORIGIN      
        1 actgaatgac gttgcccatg agcgattcag aagatccagc gaaggatacc gtttgcgagc
       61 gaaacgctgg aaatccgaac gggcgtaggg atcactcaac caccagcctt catgactgtg
      121 ctgctgatca accacccagt ccacatgtgg caatgggaaa tttccctctc cgtaggggcc
      181 attcaagcac gcatagatga tcgccacttg atctccgaag tgctcatgta cgcgacgata
      241 gaactctcga tacacgtcca gtgttctcgg agagaagaaa ctcaacacac gagttggctc
      301 atcgtgttca agacagcgca tcagctcctc tggatgcttt tcggcgtacc aggttggagg
      361 gaagtgaatc catggataga tgacatattc aatccccgct cgctcaagat gctttctcac
      421 ccggtcatgt ggttcccaat tccagactcc aggcttggat tccagagttc cccaacagag
      481 gtagtcctca aaccctttca attggtgttg acgcatcttg agcagttctt ccagggacag
      541 gtactgctcg atgaagttgg catcaggttg tcttccaatg gatgagtgtt gaatcgtcgc
      601 cacattcaga ttgcctgtgt aggcaagcga tctgtttgag atggatccaa aacccttgtc
      661 ttgaaggttc atcaactcac ctcttgttgt acatccaaac gccagcagac agaagatcgc
      721 cgacacaaag aagttggaaa tcaccacaac acgatgaaac ggcaaacgag atgttttcat
      781 ggcctctgac aatcctggtc ttttcaacaa gcgtgaactc ctccgcgccg gatatccttt
      841 gcaaacgaca gcctcacctc tatcttgcat ccatcactat ctgccgccga tgatggatgg
      901 aagccccttg gtattgccca caaaccgaag gatatcacct cggctcgtga ctccattggt
      961 cggatcgtag attctgaaca actgactggg cagagcaagc acccactctc caccatcgta
     1021 gacgtggtct gggccatagg tgccgaacat gcaggcggtt gtgggtaagc ggtcagaaac
     1081 ggcagcaatg acatctcccc aacagccagg cgtgattcca tatccgccgt tgtagtactt
     1141 gtaggaagag ggtattgccg gagtctctcc actcactgca agctgatcgt tgaatgggtc
     1201 aatgtagaca cccgtgttct tgacataggc gatgggcgaa gacagctgac ataagtctgc
     1261 gacaaaccaa ctgcttggga accctccctg aaaggcagag taatgccggt ctggcgggaa
     1321 tccgttctga tcaacgctgt aagccgtggc tgctaagtcc aacgcccgat gatcagaata
     1381 gacccgagcg acctttgccc gaatctgggc ttggatgaag ttgggaatgg caatcgcggc
     1441 taaaattccg atgatcgcga caacaataag caactcgata agcgtgaatc ctggatcgct
     1501 ggacatgatt ctctctcctt ggtcttgatc ctccgcgttt gatgcctcaa tccgtctctg
     1561 attctacctt ctctcacgag agagtcaata gtatttgcct cgtatcttgg ccaagcacgg
     1621 gtacaatctg atcccctggc agacaagtgc ttgagggtgg tggccggttc ccaggagacc
     1681 agacttccag acagcctgtc tcttccgatg agcaagcgtt cgtaagagtg ggcatagcct
     1741 gcacgcccgc aggtgctcgc ctgctcgaga tgacgatttc ttgcaccaaa cttggtgtct
     1801 ttgtgtcttg gtggtagatt ctcttcccag agcaagtagt ccgatcgcac cctgaagcgg
     1861 cttcttgggt tgcgcgcaac tcaggcagaa actgcggata cggagattcg tctgatgaga
     1921 gaaacactca ccatcgttgt tgctgtcctt ctggcttcga tttcagttca gatctgtctg
     1981 gctgacgaca cgcagcagaa atcttctgca caagtgatct ctgcgacaaa accttccttg
     2041 atcccacccc cgcgacggga aataccgggc agacgtcttg agatcgcgcc caagcaggaa
     2101 atctacgttc cggaattctt ctctcaacct gctgacgcaa cggagttcgc gctcgtcgtc
     2161 ttctttcacg gcgcgtcctg gtgtgctgag cagaatttct acgacgcgcg gaagaacgcc
     2221 gtgctcctga ctgtgaatca gaccgatccg cttcccttct cggtcatggg tcaggcactg
     2281 cctgagatcc tggagcgcac cgagcagagg ttactcgatg agggtctggt ctcaggacgg
     2341 atcagcgcac tctgtatcac ctctttcagt ggtggttatg tcgcagtaag agagatactc
     2401 aaagttcccg agatagtgaa ccgtgtgact gacgtggttc tcgcagattc cctctatgca
     2461 ccgaggattc cggaagcgac cgatcaactg gagccttccg cgctggagcc gtttttgaac
     2521 tacgcaagac aggcgtcaac agggaaggga catttttggt tctctcatct gtttcccccc
     2581 gaagagcagc atcgtggcaa tacaacaact cttgcggcgc gttatctgtt ggacaaactg
     2641 caggtgcaga tgcgatctgc ctcggacaag aattcgcgtg gagcctcgct gttgtatcgt
     2701 gcggacaaag gcaactttca tgtgctcggt tacgcaggca tgaccacgca ggatcacttc
     2761 gaacatttct atgcactcag cgatctgctg agattgacat ccattccgga cgcaacgcag
     2821 taatgctcgc cgtaagtctt tgggatggga ttctgccccg cacgagagtg ggacttcaaa
     2881 actctattcc gaaacaaatc ggaacgtttt gccggatcgg aagaggttca accgctcttc
     2941 gatgtcctgg gcaaacagat cgtctccatg tcctcgcgcg atggagaggg ctttctccgc
     3001 acaggcagcc gcctcagtga atcgtcctgc ctcggcatag atcataccca gtgtgtccat
     3061 gatgacgggg ttgtcctcgc cttctttgcg acgggcattc tctccaagtc gaacggcttc
     3121 ggcgacattt ttcagtgcag gatcgtcatt gacagccagc atcatcgcca gcttcgcttc
     3181 cagcattgcc caatcgggct ggagtcgaag cgcttcccga tagagttcca ttgcttcacg
     3241 aacccgaccg atatgagcaa gcgcatcgcc gtagcggctt cggatctccg catcttccgg
     3301 ctcggctttc ataaggtgct catagttggg aagggcttca tgaaacttcc ccgcaagtgc
     3361 aagcgcattg gccagattca ggcgggcaga tgtgaaagag ggattccatc ggagcgccat
     3421 ttggaaacgc gcgatggcat tttcgatatc tccctgctgc tgatacagag cgcccaggtt
     3481 gttgtgggcg tcggggaaaa agggtttcgc ctcgatggct ttgaggtagt gttgttttgc
     3541 ttgctcaaac ttcccctgaa atgagagtga gaccgccaaa tcgtaatgca cctccggatg
     3601 atcgggatga atctccaacg ccttctggaa gtgttctatt gcctgatcat gctctccctt
     3661 tcgggcgtag acgtttccga tgttgttgtg cgcatccgca tgattggatt ccaactcaag
     3721 tgctttctgg taatagggga aagccgattc gtcgttgccc tgaagtgcat aagaaagccc
     3781 gatgttgttg taagcgctgg cgaactttgg atcgaggctc aagactcttt ggaaatagcg
     3841 gtcggcttca tcgaagcgac ggagccgcgc cagtgccacg cccaagttgt tcatggcgtg
     3901 aagattgtcc ggatccaaac gcagtgcctg ttgatgcagt tcgattgctt tgtccagctg
     3961 tccctgatag gcaagccgaa cgccgagatt atcataggcg ataacggcct tcgggttctt
     4021 ggcgatggtg tcacgccaga gcgtttcttc gttgtggtag atatgagttt gtttccaggt
     4081 cagcaatgca aagcaggcaa ccaagaccgc cgcgcctgca ccggcaaact gacggaacac
     4141 cggcatttgt ttctgaacga gatggactgc tccccccacg gccagcgcaa caacgccgat
     4201 gagagaggtg tactgccagt gatcggcgac cagtgaatac ctcataaagt agatgttgaa
     4261 aaatcccaga acgggaaaca aggtgactgc gtaatagccc atggcgaaaa ggaaaggacg
     4321 tccccatccg tcccgtctgc gccagaaaac aatccccatg gcgacgagaa gcgctccagg
     4381 gatgtagaca atcgggttcc gtgcattgat gtcccaacgc ggatagacaa aacacagatt
     4441 gacgggaagc aaggccttgt agagatagaa ccagactgcc caacccgcgc cggcaagtcg
     4501 cgacgcaaag ccgtccgttc ggatgatatc gtccccgatg gctcggttgg actgaaacca
     4561 aatcgtcacg agggacagca caaacgacag ggcgaagaaa ggaagagtcc tcaggagatc
     4621 tttccgtgta aggcggcggt tgatccacca aaggcaaccc agcaggaata tcgggaacat
     4681 cgctaccgaa gtcttgctca gcagagagag caggaaaagg agcaaagcca gcaggtaatg
     4741 ccttcttgag aacgcatctt tgttggcgtg tgcgcgcaaa aacaaaaaga ccgagatcat
     4801 ggcgagaaac atcggcagcg tgttcttgcg ttccgtaatc caggcgacgg actccacatt
     4861 caccggatgt accgagtaca ccagcgtcgc caaccaggct cccggaattg ccagatgtcg
     4921 tagaacgcgc cagagcacca aacacgttcc cagatgcagc agcacattga cgacatggta
     4981 ccccatcgga ttcatccccc acaggcgcca ctccacccag agcatggacg acgtcagggg
     5041 aaaatagtcc ggcggttccg ttgtgaacca gaaacggtaa agcccgtctg gcgcctttat
     5101 caaatcgttg tatatgagga aatcaccatc atcccagtta aaatccgctc gcattgcggg
     5161 aatgtaagaa acaagcacaa cagagacgag aacgacggtc ttgagcacag ggatccaagg
     5221 gactctgctg agaaaccttg cggaggaaaa cggattggaa cgacttgttt cttgggaaat
     5281 ctgggtgcta cgtctgcgtc gcttgactct tgctttgctc atggcatatc aatgctcctg
     5341 ttcagagcca gttgaccctt ttcttatatg tgaacgacgt ggcatgtcaa gcagacaagg
     5401 caactgcgga ttgttttgcc tatatcgttt aagtgcggta atataatcga agtggacgat
     5461 tcggaagagg gttccagact gacatgagag atcgagagca gttttgtctc aaggagacag
     5521 gggaatgcca agtattgact ttggtgagta tcttgatcgg gtctacggtt gttggctcgg
     5581 gaagtgcatt ggcggaacgc tcggtggccc gtacgaggga atgaaggaac tcctggatg
//
LOCUS       DAOQUC010000051         5567 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_16056_length_5567_cov_2.859035, whole genome shotgun sequence.
ACCESSION   DAOQUC010000051 DAOQUC010000000
VERSION     DAOQUC010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5567
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16056_length_5567_cov_2.859035"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2020
                     /locus_tag="PKH07_01525"
     CDS             <1..2020
                     /locus_tag="PKH07_01525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33659.1"
                     /translation="IREEIPALDPASVIREVQKTLTEQTGSLTELGKAYQYTDHSLAT
                     SQPAVAQDLREVLISGLHRIEAFQSNTQETVVRILQGLGIGDTLAEGKFGLGYSAIGG
                     NSYAFPAADGLIDEGCLEFLVKPTWNPLLDQAYRVLATLVGYQPAETDLGKDAKLPFC
                     SIRVIKDTRRGLVLEVQGYDGLSESVDCPIDTWEANSWHHVAVNWSKEKGDANLYVDG
                     RFIGSTGSGMDRRSSSGYEDFGMMEGGLPRGMYTSRRSSRSASGRSTVRVLSTISLLE
                     VLYLGADHEGLNPADAVFDDLRVSNKQRTSFTFDKPSVADDTTVLLRDFEQEYIPADE
                     AELMKTLSAMRHKMVYAEDSHYNQQIRDRYAREYEVLRRSLGIDVKKVEEERAGNGVY
                     VLEELFLADYLRKHLPSSFTLDYLVQLLAFKIPEKDVLEDLVAYLDIAEWIAKKAVDQ
                     KVQVLSEVRCLGSSTEQNDEAVWIEFEKVVKELEAKYPPSERQAGVMGAMGMSPEMMM
                     MMDPAAMAEMQMMMGAEMGIDGMPYGAGARGGGDPAQRAKMDEINKLNRYLESYQKGE
                     VSPDFALERKAQEGQGGVGNVYIKRIARAFFKSNVEQFAGFLYDLEHGDYLVTVDKLN
                     VTSNEKETLECSAKIDFHFLAPTEITDEQVSGTPDSATGEVPVAPAAS"
     gene            complement(2055..>5567)
                     /locus_tag="PKH07_01530"
     CDS             complement(2055..>5567)
                     /locus_tag="PKH07_01530"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HNT33660.1"
                     /translation="ADAVRFVKQGSDYSRQYQGTWHFAWGAGYLSALQTTQTINDLRR
                     KNINTMLPQIRKTADAYYISTIEPRATNITSGYVDPLADILAKAHDTSGGKQRIEVHG
                     WFVPYRVWTGTTKPPAGHVSYRHPEWRTRNEDGSVEADIVLDPGVPEVQEYLLDVIRE
                     LVTNYPDLDGLHFDYIRYMGRTAGYHPTSVSRFQQLYHRTDIPAATDEQWGQFRRDQI
                     LALVRKAYVQCKQIRWGIKMSASTVTWLDPPLNGDFTTTRPYNDVFCDWPEMMREGIL
                     DMNCPMVYMRDHTVAQYDGFRKWTEFAGFSSGGRHVLIGPGSYLNYIENHISQLLFAR
                     DCPGVAGTLLYRYGYTSLEQNETLSWQAINGDVFDKRREVPDAPWLTNPSFGIACGTV
                     RDVTGTAVDGALLSIGTNPEIQKTDGTGFYAFVKLPAGSHSIYAHYEGKRAQGTCLVE
                     PGKVTTVDLTLDDGDSMMLRGPASFVFRGYQDGPFTPVSETTFITNTGDTQLTWTSSK
                     AQPWLVLNPTQGVLSPEQQTVVSAFWTPEASSLAPGEYSDNVVFENSETNYSETVPAQ
                     LIVMDAMELYPEHGWSARGTLGGPFSPSRQSFLLTNRGSSPLEWTAMSRKTWLDVQPS
                     AGILAPEASETVTVSINAEAGTLPPRDIPYTSGIVFSDLTHNLDYIRYCTVQVGDVAS
                     MPFWETFENGLASYWETSGSGDFRTEIRTAPDAPEGSSHLTMDSASGSTEYVRNELNL
                     TIDLSGQTNVILSFWAKGYGVEPQGVPSSPFTGSRDFNGVAVSEDGLNWHMLHSLDDL
                     PPYYTMRSIKLDDFLDARGLSYSSRFRFRFNYYGSGQVPSQGIAIDDIHIAGSVPSDL
                     VVAPAEDFYAIGDANRVFLPSSKVYTLTNSGTGEIIWSVFEDSGWFDIEPLNGVLAPG
                     ASQGVEFTVNSLARSLEPGKYLSYLFFINETAVSGQYRTAQLRVAIPDYFTEIFENSD
                     NDLSYISLAFHPDERNNTYHLCIEDKDVVQFPTNPSGGTPLILGDNTYVRVVLTDADQ
                     VSLYGKSYTSFFVGSNGYITLGQPDVETEESLLSHFTIPRISALFDDLDPIRPSASVS
                     WKRLGDRIAVTYQNVPEYNTDNRNSFQIEMFFNGTIRITYLGIAAKDGLAGLSAGLGI
                     PHDFTESDLTTALPCGCGTLLLGK"
BASE COUNT         1358 a         1376 c         1514 g         1319 t
ORIGIN      
        1 catccgggaa gagattcccg cgcttgatcc tgcttcggtc atcagggaag tccagaaaac
       61 acttactgaa cagacgggct ctcttacgga acttggaaag gcttatcagt acactgacca
      121 ctctctagcg acgagccaac cagcggttgc tcaggatctg cgagaggtgc tgatttccgg
      181 ccttcatcgt atcgaagcat ttcagagcaa cactcaggag accgtcgttc gcattctgca
      241 aggattggga atcggcgata ctcttgcgga aggcaagttt ggcttgggtt actctgcgat
      301 cggcgggaat tcctacgctt tccctgccgc tgacggcctg attgacgagg gctgtctgga
      361 gtttttggtg aagcctactt ggaatccact gcttgatcag gcataccgag ttttggccac
      421 tctcgtgggc tatcaaccgg cggagaccga tctgggcaaa gacgcaaagc ttcctttttg
      481 ctcaattcgc gtcatcaaag acactcggag aggacttgtt ttggaagtgc aggggtacga
      541 tggtctctcg gaatcagttg attgtcccat tgacacatgg gaggccaatt cctggcatca
      601 tgttgcagtg aactggagca aggaaaaggg tgatgccaat ctctatgttg acggccgctt
      661 cattggtagc actggtagcg gaatggatcg ccgctcgtca tcgggctatg aggatttcgg
      721 catgatggaa ggtggactgc ctcgtggcat gtatacctca cgacgttctt cgcgtagcgc
      781 atcgggccgg agtactgttc gcgtgttgtc cacaatcagt cttcttgaag tcctctacct
      841 tggagcggat cacgagggac tgaacccagc ggatgctgta ttcgacgatc tccgagtctc
      901 aaacaagcag agaactagtt tcacttttga taaaccctca gttgcagacg acacgactgt
      961 tctccttaga gattttgagc aggagtatat tcctgctgat gaagcagaac tgatgaagac
     1021 tctcagtgcc atgcgtcaca aaatggtata cgctgaggat tctcactaca atcagcagat
     1081 ccgcgatcgt tacgccagag aatatgaggt cttgcgtcgt tcattgggga ttgatgtgaa
     1141 gaaggtcgaa gaagagcgcg ctggaaacgg agtttacgtt ctggaggagc tgtttctcgc
     1201 agattacttg cgcaagcacc tgccgagttc cttcacgttg gactatcttg ttcagcttct
     1261 cgccttcaag attccagaaa aggacgtgct tgaagacctg gtggcgtatc tggatattgc
     1321 tgaatggatt gccaagaaag cagtggatca gaaggttcag gttctcagtg aagttcgttg
     1381 tcttggcagc agcactgagc aaaacgatga agccgtctgg atagagtttg agaaggttgt
     1441 gaaggaactc gaggcgaagt atccgcccag cgagagacag gctggagtta tgggcgcgat
     1501 gggtatgtcc cccgagatga tgatgatgat ggatcccgca gccatggcgg agatgcagat
     1561 gatgatgggg gcggagatgg ggattgacgg catgccttac ggtgctggtg caagaggagg
     1621 gggagatcct gcacagcgcg caaaaatgga cgagatcaat aaactgaacc gctatctcga
     1681 gtcctatcag aagggtgagg tcagtcctga ttttgcattg gagagaaaag cccaggaggg
     1741 gcaaggtggt gtcggaaatg tctacatcaa gagaatcgcc cgcgccttct tcaagagtaa
     1801 cgtggagcag ttcgcaggat ttttatacga cttggagcat ggcgattatc ttgtgaccgt
     1861 tgacaaactc aatgtgactt caaacgagaa ggaaaccctg gagtgctctg ccaagattga
     1921 tttccacttt cttgcaccaa ctgaaatcac ggatgaacag gtaagtggaa ctccggacag
     1981 cgcaacaggt gaagttcctg tcgcgcctgc ggcttcctga cagcactgag ttctttgtct
     2041 acgaacgcca ctgcctattt ccctagaagc agcgtaccac aaccgcaggg aagggcagtc
     2101 gtcagatcgc tttccgtaaa atcgtgcgga attccaagcc ctgccgagag acctgccaga
     2161 ccatccttag ccgcgattcc aagataggta atccgaatcg tgccattgaa gaacatctcg
     2221 atctggaaac tgttccggtt gtccgtattg tactccggaa cattctgata ggtcactgca
     2281 atccggtcgc caagccgctt ccaagaaacc gatgctgacg gacggatagg atcgagatcg
     2341 tcaaagagcg cagaaatcct tgggattgtg aagtggctca gcagggattc ttcagtttcc
     2401 acatcgggct gacccaaggt aatgtacccg ttgctgccaa caaagaaact ggtatagctc
     2461 tttccataga gactgacctg gtcagcatct gtcagaacaa ctctgacgta agtgttgtct
     2521 cccagaatca gtggggtgcc tcccgatgga tttgtgggaa actgaacgac atccttgtcc
     2581 tcaatgcaaa gatgatacgt attgtttctt tcatcaggat ggaaggccaa actgatatag
     2641 gaaagatcgt tgtcggaatt ctcaaaaatc tccgtgaagt agtcgggaat cgcgactcga
     2701 agttgggctg ttcggtattg tcccgagaca gcagtctcgt tgatgaagaa aagatagctc
     2761 aagtacttgc ccggctccag gcttcgggcc aaggagttaa ctgtgaattc cactccttga
     2821 gatgctccag gagcgagaac gccatttagt ggttctatat cgaaccaacc agagtcctca
     2881 aaaaccgacc agatgatctc accagtaccc gagtttgtga gagtgtaaac tttggaactt
     2941 gggagaaaca ctcggttggc gtcgccaatg gcatagaaat cctcagcggg cgcaaccacc
     3001 agatcgctgg gcacacttcc cgcaatatgg atatcatcaa tggcgatgcc ctgagacgga
     3061 acttgaccgc tgccatagta gttgaaacgg aatcggaaac gagaactgta agagagccct
     3121 ctcgcatcga gaaaatcgtc caacttgatg gagcgcatcg tgtagtatgg tggcagatca
     3181 tccagactgt gaagcatgtg ccaattcagt ccgtcttcac tgaccgccac tccgttgaaa
     3241 tctctgctgc ccgtaaacgg actggaagga acaccctgag gctccacacc gtatcccttt
     3301 gcccagaagg aaagaatgac attggtttga cctgacagat ctatcgtgag gttcagttca
     3361 tttctgacgt actctgttga gccactggcg gaatccatag tgagatggct tgagccttcc
     3421 ggagcatccg gcgcagttct aatctctgtt cggaagtctc cagatcctga tgtctcccaa
     3481 taggatgcaa gaccgttctc aaaagtctcc cagaatggca tggaagcaac gtcgccgact
     3541 tgcacagtgc agtaccgaat gtagtccaga ttgtgagtca aatcactgaa tacgatacct
     3601 gaagtatagg gaatatctct aggaggcaga gtgcccgctt ccgcattgat ggatacagtg
     3661 actgtttcac ttgcttcggg agccaagatt cccgcactcg gctgtacatc aagccacgtc
     3721 ttgcggctca tggctgtcca ctccaagggg gatgagcctc ggttggtcag aagaaaactc
     3781 tgccgagatg gggaaaacgg tccaccgagg gttccgcgtg cagaccagcc atgttcagga
     3841 tagagttcca tagcgtccat gacaatcagc tgcgccggaa ccgtctctga gtaattcgtt
     3901 tcggagttct caaatacgac attgtctgag tactctcccg gtgcgagaga actggcttct
     3961 ggtgtccaga aggcagagac aactgtctgc tgctctggag aaagcactcc ctgggtggga
     4021 ttcaggacaa gccaaggttg cgctttagat gacgtccaag tgagttgagt gtccccggta
     4081 ttggtgatga atgtcgtttc cgatacaggc gtgaacggcc catcctggta acctctgaag
     4141 acaaaggagg caggccctcg aagcatcatt gagtcaccgt catcgagagt caagtcaacc
     4201 gtagtaacct tcccaggttc aacaagacaa gttccttggg ctcgcttgcc ctcatagtgc
     4261 gcgtatattg agtgacttcc agcgggcagc ttcacaaaag catagaagcc cgtcccatcg
     4321 gttttctgaa tctcaggatt tgtccctata gacagcagag caccatcaac cgccgtgccg
     4381 gtgacatcgc gaactgtgcc acatgcaatc ccaaaactcg ggttggtcaa ccagggagcg
     4441 tcaggaactt ctctgcgttt gtcgaacaca tcaccattga tcgcctgcca ggaaagcgtc
     4501 tcattctgtt ccagagaggt atatccgtac cggtacagca gagttcccgc aacccctggg
     4561 cagtctcttg cgaaaagcag ttgggagatg tgattttcga tgtagttaag atagctgcca
     4621 ggaccgataa ggacatggcg tccgccgctt gaaaagccag caaactcagt ccatttcctg
     4681 aatccatcgt actgcgccac agtgtgatct cgcatgtaca ccatggggca gttcatatcg
     4741 aggatccctt cccgcatcat ttcaggccag tcacagaata cgtcattata gggacgggtc
     4801 gtcgtgaagt cgccattcag tggcggatcc agccaggtaa ccgtcgaagc ggacattttg
     4861 atgccccatc ggatctgttt gcactgaacg taggcttttc gtaccagtgc aagaatctga
     4921 tctcttcgga actgacccca ctgctcgtcg gtcgcagccg ggatgtccgt gcgatggtac
     4981 aactgctgga atcttgacac gcttgtggga tggtatccag cggtgcgacc catatatcga
     5041 atgtagtcaa aatgaagccc gtccagatcg ggatagttgg tcacaagctc tctgatgacg
     5101 tcgagaagat actcctgaac ttcaggaacg ccagggtcaa gaacgatgtc tgcttcaacg
     5161 ctcccatcct cattccgtgt ccgccattcg ggatgccgat aagagacgtg ccctgccggt
     5221 ggcttggtag tcccagtcca gacacggtag gggacaaacc agccatgcac ctcgatgcgc
     5281 tgtttgccac ctgaggtgtc atgcgctttg gcaagaatat ccgcgagagg atcaacgtag
     5341 ccactggtga tattcgtcgc tcttggttct atggtcgaga tgtaataggc atccgcggtc
     5401 ttgcgaatct gcgggagcat ggtgttgatg ttcttacgcc tgaggtcgtt gatcgtctgg
     5461 gtggtttgca gcgctgaaag gtagcctgct ccccatgcga aatgccaggt tccctgatac
     5521 tgtctggagt agtctgaacc ttgctttaca aagcgaactg cgtcagc
//
LOCUS       DAOQUC010000052         5535 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_16179_length_5535_cov_3.413686, whole genome shotgun sequence.
ACCESSION   DAOQUC010000052 DAOQUC010000000
VERSION     DAOQUC010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5535
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16179_length_5535_cov_3.413686"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1979
                     /locus_tag="PKH07_01535"
     CDS             <1..1979
                     /locus_tag="PKH07_01535"
                     /inference="COORDINATES: protein motif:HMM:NF012361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HNT33661.1"
                     /translation="KGNQLEGLKAQHPFVERESVFILGDHVTLEQGSGCVHTAPGHGQ
                     EDYLIALKYGLPAFSPVDDEGRFTEEFSMMQGRPVEEANKDIIALLADSGALVHQAKI
                     QHSYPHCWRCKQPIIFRATPQWFISLERENLREHLLDEIHKVQWIPAVGIERISGMVQ
                     RRVEWCISRQRNWGVPIPALYCKGCEEPLLDAGLIELLASLVEKCGVEVWFAETEEEK
                     AAVQPEADALSKAISSLKCAKCGSNEFEREHDIFDVWLESGASHQAVVRGRPELDYPV
                     DLYLEGSDQHRGWFQSSLTTAVGAGYSAPFKTVLTHGFVVDGEGRKMSKSIGNVIAPK
                     EVIDKYGADVLRLWVASEDFRGDIKISDEILKRLSESYRKIRNTFRFLLGNLCDHDPK
                     QAVPVAQRLELDQFIVTRLIGLKQTCRDAYEQFELHRVFQLVNNFCVVDLSAFYLDIV
                     KDRLYTWGHRSLGRRAAQTTLAEIVTTMVKLISPILCFTAEEIWQHLRKLNLVDQESV
                     HCSQYHSLEASPDTSLVERWESLLVVRSDVCRALEVKRRDGVIGHSLDSKVVLHAAET
                     SLLSLIRHYAENVQYGDDLTSLFICSQVEVSAEPLTESDAETAEHVQSLAILARRADG
                     DKCPRCWKYSTQIGQSVKYPDICPWCAQALEESL"
     gene            1979..2572
                     /gene="lspA"
                     /locus_tag="PKH07_01540"
     CDS             1979..2572
                     /gene="lspA"
                     /locus_tag="PKH07_01540"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HNT33662.1"
                     /translation="MQTDPKPCQCGCQQDGDPSGSHSDCCCLALGRRGWILLLGFLLF
                     VFVLDRVTKIWASEWLYFHGAVSVIPGLFSLRYARNTGIAFGLLSHYGTLVQWLSPFV
                     FVALVFFFIKSVHFRGLFPNWRAVAIGMVVGGALGNLYDRVVFQYVIDFLDVYVGSYH
                     WPTFNVADSFICIGVGILLILAYQTEKITVPSATDTH"
     gene            2748..>5535
                     /locus_tag="PKH07_01545"
     CDS             2748..>5535
                     /locus_tag="PKH07_01545"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNT33663.1"
                     /translation="MREKVSAALLCLAIAFVLPDAFAGEVIRLNASVFDPLEQRPETS
                     STFAVPKAFHEKNQLWLVQFHQSPTAEDIKVLEGNGISVLGYIPDDVYLVLSSVGMPE
                     IGDARVRWVGAYQPAFKMQSELAKALETDYATESDTTGDRASVVIQVPRYADYEKKIR
                     PVIEAAGGTVSRDHGVGKFVNVRAEIPLNQIPAVASEPYVIWIEKEPERQLKGERACL
                     INTGEFSSGFACPTQSGYAQWLNQYAVNGFGIIAHIADDGLDRGIDTNQPGTAHPDIL
                     GRIAKAENLTTDPSGDSRGGHGQINCGIIMGNATLGAKDKDGFLLGMGMAPSAWIYSS
                     KIFRNSGAFSMSKSFSEMLDAARQTGAVVSSNSWGADIQGVYNTDSQEYDYLVRDAAN
                     GVVGNQGMTCVFAAGNDGARSTSTNPHGYQTLDAPASAKNVIAVGASENCDADGVDAC
                     GHGPDSADNTNHLTSFTSRGPAADGRLGVTLVAPGTHINGPASTGSDFDGSSVCGFWP
                     TNQSYYVRSTGTSHSTPLVTGAAVLFYDKYMQQSASEPSPALVKAALVNGCSDMQGGL
                     DSLSFHTISPIPDPKQGWGRLNMKNVLSDEIKRIYQDQQVVFVSTGESYTIRVSPVNP
                     AEPLKISLAWTDAPAMTTAAVTLNNDLDLTVQQGSDLWLGNVFQGGRSVQGGVADSLN
                     NLECVYIQNPSGRYEVTVTAAKLVADGVPGNGHALDQDFALVISNAAEQSSEGLVLLD
                     QPVYSCQAQMKVIVSDSDIRNATGISVTLSCPQLGDLETLALLEQEVGSGIHEGIISL
                     QQGEPVVGDGFLQVADSVTCTATYRDLDDGNGEIMDVTAQALVDCVAPNISNVLVQEL
                     SLDSVRISWETDEPARALLRYGHSCSSASEESRSQALTQNHSMDLMNLDSCSMYYFKL
                     ECTDRAGNIALDSNG"
BASE COUNT         1231 a         1387 c         1573 g         1344 t
ORIGIN      
        1 ccaaaggaaa ccaactggaa ggtctcaagg cgcagcatcc atttgtggaa cgtgaatctg
       61 ttttcattct gggagaccat gtcactttgg agcaaggctc cggctgtgtg cacacggcgc
      121 cgggacatgg ccaggaagac tacctaatcg ccttgaagta cggcctgcct gcgttttccc
      181 cagtggacga cgaagggcgc ttcacggagg aattctccat gatgcagggc agacccgtcg
      241 aagaggcaaa caaggacatc attgcactgc ttgccgatag cggggcgttg gtgcatcagg
      301 ccaagattca gcattcgtat ccccactgct ggcgatgcaa gcagccgatc atcttcagag
      361 cgaccccgca gtggtttatc tctctggagc gcgagaatct tcgggagcat ttgcttgacg
      421 aaatccacaa ggtgcaatgg attccggctg ttgggatcga gcgcatctcc ggcatggttc
      481 aaagacgtgt tgaatggtgc atttcgcgac agcggaattg gggtgtgccc attccggcgc
      541 tgtactgcaa gggttgcgag gagccgctct tggatgcagg cttgatagag ttgctggcgt
      601 ctttggtcga gaagtgcggt gtggaggtct ggtttgccga aacagaggag gaaaaggccg
      661 ctgttcagcc tgaagccgac gctttgtcga aggcaatatc ctctctgaag tgcgccaagt
      721 gcggaagcaa tgagttcgaa cgtgagcacg atattttcga tgtctggctc gaatccggag
      781 cgagtcatca agccgttgtc agagggcggc cggaattgga ctatccagtc gatctctatc
      841 ttgagggcag cgatcagcat cgaggctggt tccagagttc cctgactact gcggtcgggg
      901 caggttactc ggcgccgttc aagacggtac tgacacacgg ttttgtcgtg gacggtgaag
      961 gtcggaagat gtccaagtcc atcggaaatg tcatcgcacc taaggaagtc attgacaaat
     1021 acggtgctga tgtacttcga ctgtgggttg catccgaaga ttttcgaggt gatatcaaga
     1081 tttcggatga gattctcaaa cgtctttcgg aatcctatcg gaagattcga aatacattcc
     1141 gatttctcct tggaaatctt tgcgaccatg atccaaagca ggcagtccct gtggcacagc
     1201 ggctggaact ggatcagttt attgtcacgc gacttattgg gcttaagcag acctgccgag
     1261 atgcgtatga gcagtttgaa ctccatcggg tattccaact cgtgaacaac ttctgcgttg
     1321 tggatctaag tgcgttctat ctcgatatcg tcaaggatcg gctctataca tggggacatc
     1381 gaagcctcgg aaggcgtgca gcacagacaa ctcttgccga gattgtcacg acaatggtaa
     1441 aactcatcag cccgattctc tgcttcacgg cggaagaaat ctggcagcac ctgcgcaaac
     1501 tcaatctcgt tgaccaggag agcgttcatt gttctcagta ccactcactg gaagcctccc
     1561 ctgacacttc gttggtcgag cgttgggaat cccttctggt ggttcgaagc gatgtctgtc
     1621 gcgcactgga agtcaagcgc cgtgacgggg tgatcggcca ttctctggac tcaaaagtcg
     1681 ttctccatgc ggcggagacc agtcttctga gtctgattcg tcactacgca gaaaacgtcc
     1741 aatacggaga tgatttgacg tctctgttca tctgttctca ggtggaggtg tctgcagagc
     1801 cgttgacgga atcggatgcc gaaaccgccg agcacgtgca gtctctggcg atcctggcgc
     1861 gtcgcgctga cggagacaag tgtcctcgat gctggaaata cagcactcag attggtcaaa
     1921 gcgtaaaata tcctgatatc tgcccctggt gcgctcaggc attggaggaa tccttatagt
     1981 gcagactgat cccaaaccgt gccagtgtgg ttgtcagcag gatggggatc cctctggttc
     2041 gcattccgac tgttgctgtc ttgccttggg tcgaagagga tggattctgt tgttgggatt
     2101 tctcctgttc gttttcgtgc tggatagagt cacaaagatc tgggcttccg aatggctcta
     2161 cttccatggg gcggtctctg tcatcccagg tctgttttcg cttcgatatg cccgaaatac
     2221 aggcatcgcg ttcgggctgt tgtcccatta cggcacgttg gtgcaatggc tttcgccatt
     2281 tgtttttgtg gcgttggtgt tcttcttcat taagtcggtt cactttcggg ggcttttccc
     2341 caattggcgg gcggttgcca ttgggatggt tgtcgggggg gctttgggca acctctatga
     2401 tcgtgtcgta tttcagtacg tcatagactt tctggatgtc tatgtcggca gttatcactg
     2461 gccaacattc aacgtcgcgg actcgtttat ctgcatcggt gtcggtattc ttctcattct
     2521 cgcctaccaa actgagaaga taactgttcc gtcggcaacc gacacccact gacagtcatc
     2581 ctgtgaacga ccctgcctga ccgaaattga cagtttcaca ggcggcgttg tactctcaat
     2641 cctgtgtcgt ctttctagtg ctcttgagat gaaggttgct catctctttc gtacaaagcg
     2701 acgggataca gaaagggaaa tccatgacaa gtgagagaac acgaataatg cgagagaagg
     2761 tctctgccgc tttattgtgc ctggctattg catttgtgct accagacgcc tttgcgggtg
     2821 aagtgatacg cctcaacgcc tctgtttttg acccgcttga acaaaggcct gagacatcaa
     2881 gcacctttgc agtacccaaa gcattccatg agaagaatca actctggttg gttcagtttc
     2941 atcagtctcc aacagcagag gatatcaagg ttcttgaagg aaacggcatc tcggtgttgg
     3001 ggtacattcc tgatgacgtc tatctggttc tgtcctcggt cggaatgccg gaaatcggcg
     3061 atgcgcgtgt gcgttgggtg ggtgcctatc agccggcgtt caaaatgcag tcggaacttg
     3121 cgaaagccct tgaaaccgat tatgcgactg aatccgatac cacaggtgac agagcctcgg
     3181 tggtgattca agttccccgc tatgccgact atgagaagaa gatcaggccg gtgatcgaag
     3241 cagcgggtgg aaccgtctct cgtgatcatg gcgttgggaa attcgtcaat gtacgagccg
     3301 agattcccct caatcagatt cccgctgtgg catctgagcc gtatgtgatc tggatcgaga
     3361 aggaaccgga gagacaactc aagggggaac gagcctgcct gattaacacg ggtgagttct
     3421 cctcaggatt cgcctgtcca acgcagtccg gttacgctca atggttgaat cagtatgcgg
     3481 taaacggatt cggcatcatt gcccatattg ccgatgatgg cttggatcga ggtattgaca
     3541 ccaaccagcc aggaactgcg catccggata ttcttgggcg tattgcgaag gccgagaatc
     3601 tgacgactga tccgagtggc gacagtcgcg gggggcacgg gcagatcaac tgcggaatca
     3661 tcatgggcaa tgcgacactg ggagccaaag acaaggacgg attccttctg ggcatgggaa
     3721 tggcaccttc agcctggatt tattcatcaa aaatcttccg caacagtgga gcattcagca
     3781 tgagcaagag tttctctgaa atgcttgacg ccgcgcggca gaccggtgcc gttgtcagtt
     3841 ccaacagttg gggtgccgac attcagggag tctataatac cgactctcag gaatatgatt
     3901 acctggtgcg agatgctgcc aatggcgttg tcggaaacca agggatgacc tgcgtattcg
     3961 ccgcgggcaa tgacggagcc cgcagcacaa gcaccaaccc gcatggatac cagacgctgg
     4021 atgcccctgc ctccgcaaag aatgtcattg cagtgggtgc cagtgagaac tgtgatgcgg
     4081 atggcgtgga tgcctgtggg catggccccg attccgcgga caacaccaat catctcacct
     4141 cattcacttc cagaggccct gcagcagatg gccgactcgg ggtgacactg gtcgctcctg
     4201 gaacacacat caatgggccg gcttcgaccg gatcggattt cgacggcagt tctgtctgcg
     4261 gcttctggcc aacaaatcag tcgtactatg ttcgaagcac aggtaccagc cactcgacac
     4321 cccttgttac gggcgcggct gttctgttct acgacaagta catgcagcag tctgcatccg
     4381 agcccagtcc ggcgttggtc aaagcggcgt tagtcaatgg ttgctccgac atgcagggcg
     4441 gtctggactc gctttccttt catacgatca gtccgatccc cgaccccaag caggggtggg
     4501 gaagactcaa tatgaagaat gtcctttcgg atgagatcaa gcgtatctat caggatcagc
     4561 aggtggtctt tgtctcaaca ggtgagtcct atacgattcg tgtcagcccc gtgaatcctg
     4621 ctgagccact caagatcagt ctggcatgga cggacgcccc agccatgacc actgcagcag
     4681 tcacgttgaa caacgatctg gatctgacgg tgcaacaggg ctctgatctt tggctcggaa
     4741 atgtctttca gggaggaagg tcggttcaag gaggagtggc ggacagcctc aacaatttgg
     4801 agtgtgtcta cattcagaat ccttccggtc gctatgaggt tacggtcacg gcggcaaaac
     4861 tggttgcaga cggcgtccca ggaaatgggc acgccttgga tcaggatttt gcattggtga
     4921 tctccaatgc tgccgaacag tcctcagaag gtttggtgct gttggatcag ccggtctaca
     4981 gctgtcaggc tcagatgaaa gtcattgttt ctgacagcga tatccgcaac gcgacgggta
     5041 tctccgtaac gctgtcttgt ccccaactcg gcgatctgga aacactcgct ctgcttgagc
     5101 aagaggtggg cagtggtatt catgaaggca tcatttctct ccaacaggga gagccggttg
     5161 tcggggacgg ctttcttcag gtcgcagata gtgtcacctg tactgcaaca taccgcgact
     5221 tagacgacgg caatggggag attatggatg tgacggcaca ggcgctggtg gactgtgttg
     5281 cccccaacat ctccaatgtg ttggtgcagg aactttccct cgacagcgtg cgcatttcct
     5341 gggagacgga tgaacctgcc cgagcgctgc ttcggtacgg tcattcctgc agcagcgctt
     5401 ctgaggagag ccgatcccag gcgttaacgc agaatcattc gatggatttg atgaatctag
     5461 attcctgttc aatgtactat ttcaaactgg aatgcaccga cagagcaggc aatatcgcgc
     5521 ttgatagcaa tggtg
//
LOCUS       DAOQUC010000053         5480 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_16382_length_5480_cov_3.096959, whole genome shotgun sequence.
ACCESSION   DAOQUC010000053 DAOQUC010000000
VERSION     DAOQUC010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5480
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16382_length_5480_cov_3.096959"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1492
                     /locus_tag="PKH07_01550"
     CDS             <1..1492
                     /locus_tag="PKH07_01550"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HNT33664.1"
                     /translation="KYGVDGRYDDILRGKPCQYITAMDALSRPMFLDSSPPPRSPKGA
                     DIELTLDEFIQSVAEQELDEACKSSGAPGGCVLVMDPNNGEILAMAQYPRFDPNEYSK
                     YPMANYRNRAISDAFEPGSLAKCFTMYAALAMGVTRPDTVIRCEAGFSVGRKVIKDVP
                     GTRYGDLTATEILAKSNNVGTVKLALQCGEEALYQTFREFGMSGRTGVDLPSEGVCTI
                     PRLSQWSKTTIAVLPYGYEMMATPIGLCRAYAMIANGGRAITPHVVRAVVNNETHQRL
                     EPKQPYADQILSSEKTKMLAEMMTQCIEIGTGRHAKVPGFSGRIAAKSGTAVQLERDE
                     EGRGRGYSKDKVVASFIGFLPVEAPRAVISVHIWEPDAEGPTHGGELAGPVFAKLADY
                     VATRMNMPRNTSEIETPTSTPNSLAINKQKNQTPTPSVKDYLDQGVMPDVKGMTKGQV
                     YELLKTLNLEGRFEGFGLACSQQPEPGESLEQIQVCHVNFSDEQLR"
     gene            1521..2987
                     /locus_tag="PKH07_01555"
     CDS             1521..2987
                     /locus_tag="PKH07_01555"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HNT33665.1"
                     /translation="MRALSEILQRVTTLEVAGIPSSEVIGIACDSRHVEPGMLFVCLR
                     GQHVDGHDFLEKALQAGATCALVEQWRPSVPLPQIRVACTRKALAEVSCAFYGSPAEA
                     MTLVGITGANGKTSSAFLLESILKAAGQKVGVIGTVTYRWFDRQLPAPNTTPLALELQ
                     QTLFQMKQDGVQTVVMEVSSHALALDRIHGLQYQVALFTNLTQDHLDFHETMESYQKA
                     KERLFFEYLAPEGTSVINVDDTAGASIASRVPRNHLLTYALDTPANISVKALQMDREG
                     IRADVQLPDGQRTCIQSNLLGRYNVYNLLGVTGMAFALGVNPNCIANGIREMKLIPGR
                     LEEIPNDRGLKVLVDYAHTEDALKQVLSTLKQIPHRKIILVFGAGGDRDKTKRPRMGA
                     VAAQLSDFAVLTSDNPRSEDPQQILRDIQTGMAQAKGRFEVIEDRAEAIRRAIQMAAS
                     DDIVLIAGKGHEQTQTLADRVIHFDDREQACAALKELK"
     gene            2984..4405
                     /gene="murF"
                     /locus_tag="PKH07_01560"
     CDS             2984..4405
                     /gene="murF"
                     /locus_tag="PKH07_01560"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HNT33666.1"
                     /translation="MKLSIADVANWTGGHLLKGEAAGTVTAISTDSRKTQSGEAFLAL
                     RGENFDGHQFATVAARAGAGLVILEENHPTVASAMNEFPAVIVVRDTLVAYGEIAKGY
                     RKQFSLRVLALTGSVGKTSTRGFIASVLRQKFRVLESEKNFNNLIGVPQTLLNLKDEH
                     ERAVLELGADRPGEIPRLTEMCRPRIGVLTGVFPCHLERFGSLDVIVEEKGMLLSGLQ
                     GDGTTGLIGSDSYGLDRLKSLCRGCTLTYGAAEGDFHASDIVLRNDGTTDFSAVSKER
                     KVAVHLRVLGEHQIKNAVAAFAVGCLENFPDEIIKQGLESYNGAWGRLERIDLPSGAT
                     LLKDVYNANPNSTRAALNLLGSLPARKRIAVLGDMMELGDDSESFHREMGAMVAQSEL
                     DMLLTVGERSQAIAEQAKADGFRGTVQQCSSNGAVSDLLKTEIRKGDVVLLKASRAMK
                     FEEISEHLEKTPQGESCKEGSNA"
     gene            4398..>5480
                     /gene="mraY"
                     /locus_tag="PKH07_01565"
     CDS             4398..>5480
                     /gene="mraY"
                     /locus_tag="PKH07_01565"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013705599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HNT33667.1"
                     /translation="MLRLLFYPFRESQILFNLLGYITFRAIAAAVFSLLIGIWAGPRF
                     IRWLIRFRIGQQIRGEGIPSLYEHHKAKAGTPTMGGILLLSLVLLSVILWGDLTNRLL
                     ILMIFVTVWLGLVGFLDDYIKMRRKSHRGLNKRQKLIGQFSIGLLVGLYLYAWPVDPN
                     NPTGLALPFLKHFIPELGILYIGFVALVLVSTSNAVNITDGLDGLATGCVTIASAIFA
                     IVAYLVGRVDASRYLYVLYIKGAGELSVFCAALLGASLAFLWFNCYPAQVFMGDVGAL
                     ALGGALGCVAVLCKQELLLLIVGGVFVIEAASVILQVISVRRWGKRIFLIAPLHHHYQ
                     IKGWHEVTIVTRLWILAIVFGMLGLCT"
BASE COUNT         1280 a         1389 c         1551 g         1260 t
ORIGIN      
        1 aaagtatggt gtagatgggc gctatgacga cattctgaga ggcaaaccct gtcagtacat
       61 cacggcgatg gatgcgctgt cgcgtcccat gtttctggat tcaagcccgc ccccccgctc
      121 gcccaaaggg gcagatatcg aactgactct tgatgagttc atccaatcgg tggctgaaca
      181 ggagcttgat gaggcctgca agtcctccgg agcaccagga gggtgtgtcc ttgtcatgga
      241 tccaaacaac ggagagatcc ttgccatggc gcagtatccc cgcttcgatc caaacgaata
      301 ctccaagtac ccaatggcaa actatcgcaa ccgagccatc agcgacgctt tcgaaccggg
      361 aagccttgcc aagtgcttca caatgtacgc agcgcttgcc atgggtgtga caaggccgga
      421 cacagtgatt agatgcgagg caggattcag tgtcggcaga aaagtcatca aagatgtgcc
      481 tggaacccga tacggtgatc tgaccgcgac agagattctg gccaagtcaa acaatgttgg
      541 aaccgtcaag ctggcgcttc agtgcggtga ggaggctctc taccaaacat ttagagagtt
      601 cggtatgagt ggcagaactg gcgtggatct gccgtcggaa ggcgtctgta ccattcccag
      661 actgagccag tggtcaaaaa caaccatcgc tgttcttccc tatggctatg agatgatggc
      721 aacgcccatc ggtctatgtc gtgcatacgc catgatcgcc aacggtggtc gcgctatcac
      781 gccgcatgtc gttcgtgcag tcgtgaacaa tgaaacgcac caacgccttg aaccaaaaca
      841 accctacgct gatcagatcc tctcgtctga gaaaacaaag atgctggctg agatgatgac
      901 gcaatgcatt gagatcggta ctggacgcca cgcaaaggtt cccggtttct caggtcggat
      961 cgctgcaaaa agtggcaccg ctgttcaact ggagagagat gaagaaggac gtggtcgggg
     1021 atattcgaag gataaggtgg ttgcatcctt cattggcttt ctgccggttg aggcaccacg
     1081 ggccgtaatc agtgttcaca tctgggaacc agatgctgaa gggccaactc acggcggtga
     1141 actcgcgggg cctgtcttcg caaaactggc ggactacgtt gcgacgcgaa tgaatatgcc
     1201 gagaaacacc agcgagatcg aaacgccaac ctcgactccg aatagcctgg cgattaacaa
     1261 acagaagaat caaacaccaa ctccatcggt caaagactat ctcgaccaag gcgtcatgcc
     1321 tgatgtcaag ggaatgacca agggacaggt gtatgaactc ttgaagaccc tgaatcttga
     1381 aggacgtttt gagggcttcg gcctggcctg ttcacaacaa ccggaaccgg gagaaagtct
     1441 tgaacagatc caggtctgtc acgtcaattt cagtgatgag cagttgcgct agatgaagga
     1501 tgccctacac gagtgattag atgcgagcat tgagtgagat tctacaacga gtgacgactc
     1561 tcgaggttgc cggaataccc tcctctgagg tcattggtat tgcctgcgat tcgcgtcacg
     1621 ttgagccagg aatgctcttt gtctgtctac gcggccagca tgtagacggt catgatttcc
     1681 ttgagaaggc tctacaggct ggtgccacct gcgcccttgt cgagcagtgg agaccctcgg
     1741 tacctctccc gcagatccgt gttgcctgta cccgcaaggc gctggctgaa gtcagctgtg
     1801 ccttctatgg ttcgccggca gaggccatga ccttggtcgg gatcacaggg gcaaatggaa
     1861 agacaagttc ggcctttctt ctggaaagca tcctgaaggc cgcaggacag aaagtcggcg
     1921 ttatcggtac cgtcacctat cggtggttcg acagacagct gcctgctccc aacacgactc
     1981 cactggcgct cgagttacag cagacgctct ttcagatgaa gcaggatggc gtacagacgg
     2041 tcgtcatgga agtctcttct cacgcactcg ccctggatcg aatccatggg cttcagtacc
     2101 aggtagcact gttcacaaat ctgactcagg atcatctgga cttccatgag acaatggagt
     2161 cctaccaaaa agccaaggaa cgcctcttct tcgaatacct tgcacccgaa ggaacatccg
     2221 tgatcaacgt agatgatacc gcgggcgcat caattgcttc acgagttccc aggaatcacc
     2281 tccttaccta tgcgctggat actcccgcga acatctctgt caaggcattg cagatggatc
     2341 gagagggcat tcgtgcggac gtgcaactgc cggacggaca gaggacctgc attcagtcaa
     2401 atcttttggg gcgctacaac gtctacaacc ttctcggcgt cacgggaatg gcttttgctc
     2461 tcggtgtgaa tcccaactgt atcgcaaatg gaattcggga gatgaaacta atccctggtc
     2521 gcctggaaga aattcccaat gatcggggct tgaaggtgct tgtggactat gcgcacacag
     2581 aagacgcctt gaaacaagtg ctctcaactc tgaagcagat tcctcataga aagatcatcc
     2641 ttgtcttcgg agcaggcggc gatcgtgaca agacgaagag acccaggatg ggtgcagttg
     2701 cagcacaact cagtgacttt gcggtgctga cgtctgacaa tcctcggtct gaagatccgc
     2761 aacagattct ccgagacatt cagacaggca tggcacaggc aaagggtcgc tttgaagtca
     2821 tcgaagatcg tgcagaggca attcggcggg cgatccaaat ggccgcgagc gatgacatcg
     2881 ttctgatcgc gggaaaagga catgagcaga cgcagacact tgcagacagg gtcattcatt
     2941 ttgatgaccg cgaacaggcc tgtgctgcgt tgaaggaact gaaatgaaac tgagcattgc
     3001 ggatgttgcg aactggactg gcggtcacct gctgaagggg gaagcagcag gaaccgtcac
     3061 agccatctca acagacagcc ggaagactca atctggcgaa gcgtttctgg cgcttcgagg
     3121 tgagaacttc gacggtcatc agttcgcaac ggttgcagct cgcgcgggag caggactcgt
     3181 cattcttgaa gagaaccacc cgacagtcgc ctctgcgatg aatgagtttc cagcagtgat
     3241 tgtggtcagg gatacacttg tcgcctatgg ggagatagcg aagggctatc gaaagcagtt
     3301 ttctttacga gtgcttgctc tgacaggctc ggtcggcaag acgtccacac gcggattcat
     3361 tgccagcgtt ctgcggcaga aatttcgggt tcttgagagc gagaaaaact tcaacaacct
     3421 catcggagtt ccacagactc tcctcaattt gaaagacgag catgagcgag cggttctgga
     3481 acttggcgcg gatcgccccg gagagattcc tcgactgaca gagatgtgtc gtcctcgtat
     3541 cggtgtgttg acaggcgtct ttccatgtca tctggagcgc ttcggttcac tcgacgtcat
     3601 cgtcgaggaa aaggggatgc tactgagcgg actgcaaggc gatggaacga caggcttgat
     3661 cggttcggac agctatggcc ttgatcgcct gaagagtctg tgtcgtggct gtaccctgac
     3721 ctacggcgct gccgaggggg atttccacgc ttcagatatt gtgcttcgga atgacggcac
     3781 gacggatttc agcgctgtct caaaagagag aaaagtcgcg gttcatctgc gcgtgctggg
     3841 tgaacaccag atcaagaatg cagtagccgc ctttgcggtc gggtgtttgg agaatttccc
     3901 tgacgaaatc atcaaacaag gtttggaatc gtacaatggt gcgtggggac gactggagcg
     3961 aatcgatctg ccttctggag cgacgttgct gaaggatgtc tataacgcca atcccaattc
     4021 cacacgagca gcactgaatc tcctgggctc cctacccgct cgaaaacgca ttgcagttct
     4081 tggtgacatg atggaattgg gcgatgattc cgaatccttt catcgggaaa tgggtgcgat
     4141 ggtggctcag tcagaactgg atatgttgtt gacggtgggt gagcgttcgc aagcgattgc
     4201 agaacaggca aaggccgatg gcttcagagg cacagttcaa cagtgttcca gcaatggtgc
     4261 ggtttccgac ctgctgaaga cggagattcg caaaggggat gttgttctgc tgaaggcctc
     4321 cagagcaatg aagtttgagg aaatatccga gcatcttgag aaaacaccgc aaggggaatc
     4381 ctgtaaggag ggaagcaatg cttaggctgc ttttctaccc gtttcgggaa agtcagattc
     4441 tctttaacct gttggggtat atcacatttc gagccatcgc ggcagcggtg ttctctttgc
     4501 tgatcggaat ctgggcggga ccacgtttca tcagatggct gattcgattt cgaattggac
     4561 agcagattcg tggcgagggg attcccagcc tatacgagca tcacaaagcc aaggcgggta
     4621 cgccaaccat gggcgggata ctcctgctct ccctcgtcct tctttccgtc atactttggg
     4681 gagatctcac caaccggctt ctgatcctga tgatcttcgt tacggtctgg ctcggccttg
     4741 tcggctttct ggacgactac atcaagatga ggcgcaagag ccatcgtggc ctgaacaaaa
     4801 ggcagaagct gatcggccag ttctccatag gcctgttggt cggtctgtat ctctacgcct
     4861 ggcctgttga tccaaacaat ccgacagggc tggcattgcc cttcctgaag cacttcatcc
     4921 ccgagctggg tattctctat atcggatttg ttgctttggt gctggtgagc acctccaatg
     4981 cggtgaacat caccgatggc ctggatggtt tggcgactgg atgtgtcacc atcgcttcag
     5041 ccatcttcgc aatcgtggcc tatcttgtgg gtcgcgtaga tgcgtcacga tatctctatg
     5101 tgctttacat caagggcgcg ggtgagttgt ctgtcttttg cgccgctctt ctcggggcaa
     5161 gtcttgcgtt tctttggttc aactgctatc ccgcgcaggt gttcatgggg gatgttggtg
     5221 cgcttgccct cggtggtgcg ctcggctgcg tggctgttct ctgcaagcag gagctacttc
     5281 tgttgatcgt cggaggcgta ttcgtcatcg aagccgcctc ggtgattctt caagtgatca
     5341 gtgttcgcag atggggaaaa cggatattcc tcatcgcccc actgcatcat cactatcaga
     5401 ttaaggggtg gcatgaggtc accattgtca cgaggctttg gattttggcg atcgtcttcg
     5461 gaatgttggg actgtgcaca
//
LOCUS       DAOQUC010000054         5448 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_16521_length_5448_cov_3.147228, whole genome shotgun sequence.
ACCESSION   DAOQUC010000054 DAOQUC010000000
VERSION     DAOQUC010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5448)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5448)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5448
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16521_length_5448_cov_3.147228"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..652)
                     /locus_tag="PKH07_01570"
     CDS             complement(<1..652)
                     /locus_tag="PKH07_01570"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HNT33668.1"
                     /translation="MAKQKKTLTTGFGMPVDNDLNSSTAGPKGPVLVQDVHLIEKLAH
                     FDRERIPERVVHAKGAGAHGYFEVTNDVSAYTRAKFLSKVGKKTDVFVRFSTVGGEKG
                     SADSERDPRGFAIKFYTEEGNYDMVGNNTPVFFIRDPLKFPDFIHTQKRNPATNLKDP
                     DMFWDFLSLTPESIHQVTILFSNRGTPKDYRHMNGYSSHTYMWYNAKGEYFWVQLHF"
     gene            complement(672..1142)
                     /locus_tag="PKH07_01575"
     CDS             complement(672..1142)
                     /locus_tag="PKH07_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015949740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HNT33669.1"
                     /translation="MKLTADEVKDRMRHLEECLRERGIKVTHQRLEIFREVAQSGGHP
                     DVERIYRGVRKRVPTVSLDTVYRTLLLLLDLGLLTTLGPPRDRARFDANMKTHHHFVC
                     SKCGVTQDFYHEAFNQLKIPDRVQEWGRMERTQVEIKGTCEKCLRNIGKGSEKK"
     gene            complement(1267..1785)
                     /gene="folK"
                     /locus_tag="PKH07_01580"
     CDS             complement(1267..1785)
                     /gene="folK"
                     /locus_tag="PKH07_01580"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HNT33670.1"
                     /translation="MSIVFIGYGSNLRGRLQWIERGLEMLEDALGPARKSPVYETNPV
                     GEGLTRWFLNGVWMFQTERSPHEVHRILRDAEIACGRIASVSPLAGDACTTKPQYQDR
                     TLDLDLLLYDQLILDEDNLIVPHPRMHLRKFVLKPLADLAPLTVHPLLKSTVSQLLRE
                     GDFEGQEIRSYP"
     gene            complement(1914..2402)
                     /locus_tag="PKH07_01585"
     CDS             complement(1914..2402)
                     /locus_tag="PKH07_01585"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HNT33671.1"
                     /translation="MIMAKRFSRLIDPDSIAFPMRASSKWEAIEELVALLAARGKVTC
                     TSELVEATLKRESQGSTGIGQGIAVPHAKCEAVRELSVCLGLAQPALEWEAIDGKPCD
                     VIFLMAAPPGASGPHIQSLASLARLLKATSLYDRILRATSAHEAYQALIEEENSLLAD
                     SE"
     gene            complement(2556..3029)
                     /locus_tag="PKH07_01590"
     CDS             complement(2556..3029)
                     /locus_tag="PKH07_01590"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNT33672.1"
                     /translation="MERILVAVSGTEASIVAAQYAICLAKMLQSELIAVFVVDIGTLQ
                     DLLTARIFVQSESLEYERDLEEDGRRYLNYVSKLGSEKGVAIRTEMARGQVHTEVLNA
                     ARTNEVNLIVLGEITPMLSRLETQLGQSERILYDAPCPVLVVKDEETVVEMYESL"
     gene            complement(3044..3685)
                     /locus_tag="PKH07_01595"
     CDS             complement(3044..3685)
                     /locus_tag="PKH07_01595"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016471 - vacuolar proton-transporting
                     V-type ATPase complex [Evidence IEA];
                     GO_function: GO:0008553 - P-type proton-exporting
                     transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HNT33673.1"
                     /translation="MAKKGIVPTKSKLLALERELSLAKQGHELLEQKRQILVMELMRI
                     VGRAEEVERRADESLAKAYRAMSLTFATMGKSRVKSMAQAVSINPSLSTQNRSIMGAI
                     VPAIRLALNDQPPYFAHGGASLWTDEAVESFKEALQRLTELAELKTTALRLAREVSKT
                     LRRVNALEKVSIPDHIESISYIRSVLEESERERFGSMKLIKARLGAERGGEAS"
     gene            complement(3709..5112)
                     /locus_tag="PKH07_01600"
     CDS             complement(3709..5112)
                     /locus_tag="PKH07_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005035427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HNT33674.1"
                     /translation="MNPSASTAPETGIQYVGLEKVDGPLVVIKGIHNIGWGELVEVED
                     SSGRLRMGNVLEASDGAAIIQVFDGTYGLQMNRTKARFTGECLRIPASEQMLGRVFNG
                     LGQPQDGGPPPITKARWDVNGLPINPTARDYPRKYIHTGISAIDALNTLVRGQKLPIF
                     SGSGIAHNTLAAQIVRQARIVGEDLQFAVVFGAMGIKHDVARFFMDSFESSGVMEKVV
                     VFLNLADDPPIERIITPRAALTVAEYLAFEADMHVLVILTDMTNYCESLREVSVVRGE
                     IPSRKGYPGYLYSDLASLYERAGMIKGKTGSITQMPLLTMPNDDISHPVSDLSGYITE
                     GQIVLDHELHQKGVYPPIACLPSLSRLMKDGIGPGMTRDDHAPVASQLFAAYSHVKDI
                     RALASVIGEEELAPLDKKYMEFGVAFESRFLAQSSTESRSMDETLDLGWSVLSILPRE
                     ELYRIPDEMIAKHYGRH"
     gene            complement(5102..>5448)
                     /locus_tag="PKH07_01605"
     CDS             complement(5102..>5448)
                     /locus_tag="PKH07_01605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HNT33675.1"
                     /translation="LVGADVLPDAQRWVLEVCQIFRNAFLQQNSFDKVDMFCVAQKQA
                     KMLSIITNLYRQGVANIQKGVTYLNLRKSKVIPKIAKMKSTITNDQLAELDKIDTEME
                     RSMERLGAIYES"
BASE COUNT         1245 a         1543 c         1350 g         1310 t
ORIGIN      
        1 tgaagtgcag ttgcacccag aaatactcac ctttggcgtt gtaccacata tacgtgtgac
       61 tgctgtagcc gttcatgtga cggtagtcct tcggggtgcc tcgattcgaa aacagaatcg
      121 tcacctgatg aatggactcc ggagtcaagg aaaggaaatc ccagaacata tccggatctt
      181 tgagattggt ggccgggttg cgcttctggg tgtgaatgaa gtcgggaaac ttgagtggat
      241 cacggatgaa gaagacgggc gtgttgttgc caaccatgtc atagtttcct tcttccgtat
      301 agaacttgat ggcaaatcca cgtggatccc gctccgagtc cgcggagccc ttctctccac
      361 caacagtcga gaaacgaaca aaaacatcgg tcttcttccc cactttggag agaaacttgg
      421 cacgggtgta cgcgctgaca tcattggtca cctcgaagta accatgtgca ccggctccct
      481 tggcgtgtac gacacgctca ggaatacgct cccgatcaaa gtgcgccagt ttctcgataa
      541 ggtggacatc ctgtacgagc actggcccct tcggccccgc agttgacgaa ttgagatcgt
      601 tgtcaactgg catcccaaat cccgttgtca gtgtcttctt ttgctttgcc atggctgcct
      661 cctccaaacg attacttctt ctcagagcct ttccctatgt tccttaagca cttctcacaa
      721 gtgcccttta tctcgacttg cgtcctttcc atccttcccc actcctggac tcgatctgga
      781 atcttcaatt ggttgaacgc ttcatggtaa aagtcctggg tcacaccaca ttttgagcac
      841 acaaagtggt ggtgggtctt catgttggca tcgaatcgcg ctcggtcgcg tggagggcca
      901 agcgtggtga gcaaaccaag atcgagaagc agcaagaggg tgcgatacac cgtgtcgagg
      961 gagacagtgg gaactctctt acgaactccc cgatagatcc tctccacatc aggatggcca
     1021 ccggactggg caacttccct gaaaatctca agacgctgat gagtgacctt gatgccacgt
     1081 tccctaagac actcttcaag gtgcctcatc cgatccttga cctcgtcagc ggtaagtttc
     1141 atcaggatat catcctatgt aagaattatt cttatttagg aatgtagcag atgcggatcc
     1201 ggatttcaag tggattgttc gagtgtcgga gtccgggtca gtgatactca aaggtcaaga
     1261 gtatgctcag ggatagcttc gaatctcctg cccttcgaag tctccttccc gaagcagttg
     1321 ggagaccgtg ctcttgagca acgggtgtac tgtcagaggc gcgagatccg cgagaggctt
     1381 cagaacgaac ttcctcaaat gcattctggg atggggtacg atcaggttat cctcatcaag
     1441 aataagctga tcatagagta gcagatccag gtcaagtgtt cgatcctgat actggggttt
     1501 tgttgtgcac gcatcacccg ccagtgggct taccgaggcg atacgaccac aggcaatttc
     1561 cgcatccctg agaatgcggt gcacttcatg gggacttctc tccgtttgaa acatccagac
     1621 accattcaga aaccaacgag tcagcccctc accaacgggg ttggtctcgt agacaggtga
     1681 ttttcgcgcc ggcccgagtg cgtcttccag catttccaag cctcgctcga tccactgtag
     1741 tctccccctc aggttggagc cataaccgat aaacacgatt gacatgagtg acagtcttcc
     1801 tcttgataag tgtctcgaaa aagggatgat tctcacctca gacattcctg cacagttggc
     1861 atccccggag ggactctcag gtgactgagt tgcgccggtc ggtacccagg ccgctattcg
     1921 ctgtcggcaa gaagggaatt ctcctcttca ataagggcct gataggcttc atgagcagac
     1981 gtggcacgaa gaatcctgtc gtacaaactg gttgccttca aaaggcgtgc caatgatgcc
     2041 agcgactgaa tgtgcggccc tgacgctcca gggggcgctg ccatgagaaa gatcacatcg
     2101 cacggtttgc catcaatggc ttcccattca agcgctggct gcgccagccc cagacagact
     2161 gaaagctcgc ggacagcctc gcacttcgca tgaggaacag cgatcccctg accgatgcct
     2221 gtgcttcctt gcgactcacg cttcaatgtc gcctcaacaa gttcggatgt gcacgtgacc
     2281 ttccctcttg ctgcaagaag agccacaagt tcctcaattg cctcccactt cgatgaagcg
     2341 cgcatgggga aggcgataga atcaggatcg atcagtcggc tgaatcgctt tgccataatc
     2401 atctcctttg ctggccggtt tgccgcccac gttctaacct cattggatgc gtgtctgcat
     2461 ccaccacggg tctccagatt tggacagcaa aatccgattc atcttctgcc ccttttcatc
     2521 gagcagattg tggtgttcca tgctccgtga tgggtctaca gactttcata catttccaca
     2581 acggtttcct catctttcac taccagaaca ggacagggcg cgtcgtatag aatgcgttca
     2641 gactgaccca gttgcgtctc cagtcggctg agcatgggcg tgatctcgcc gagcacaatc
     2701 aggttcactt cgttggtgcg cgccgcattg aggacttccg tatgtacctg tccgcgagcc
     2761 atctccgttc tgatggcaac ccccttttca ctaccgagtt tggacacata gttcaggtac
     2821 cttctgccgt cttcctccag atctctttcg tactccagac tctcagactg aacgaaaatg
     2881 cgcgcggtga gaagatcctg gagcgttccg atgtccacaa cgaagaccgc aatcaactcg
     2941 ctttgaagca tcttggcgag acaaatcgcg tactgggctg ccacaattga cgcctcagtg
     3001 ccactgacgg caacaagaat tctctccatc ggcttcttcg ggcctatgat gcttcacccc
     3061 ccctctcggc acccaggcgt gccttgatca gtttcatcga gccaaaccgc tcccgctctg
     3121 attcctccag aacggaccgg atgtaggaaa tgctttcgat gtggtcggga atggagacct
     3181 tctccagcgc gttgacacga cgcagcgtct tgctcacttc acgggcaagc ctgagcgccg
     3241 tcgtcttcaa ctctgccagc tccgtcagtc gctgcagcgc ctctttgaaa ctctcaacag
     3301 cttcatccgt ccacaacgat gcaccgccgt gggcgaagta cggtggctga tcattgagcg
     3361 ccagccgaat tgcgggtacg atggcgccca tgatgcttcg attctgtgtg gacagtgatg
     3421 gattgatcga aaccgcctgc gccatgctct tgactcgtga cttgcccatg gtggcaaacg
     3481 tcaggctcat ggctctgtag gcctttgcaa gagattcatc cgcgcgccgt tccacttctt
     3541 cggcacgtcc aacgatgcgc ataagttcca tgaccaaaat ctgccttttc tgctccaata
     3601 gttcatgccc ctgcttggcg agtgacaatt cccgctccag ggcaagcaac ttggatttgg
     3661 ttggaacaat gcctttcttg gccatttctc agcgactccc agaaatcctc aatgcctccc
     3721 gtaatgcttc gcgatcatct cgtcaggaat ccgatagagc tcctctcgcg gaagaatcga
     3781 aagaacactc catcctagat ccagtgtctc atccatggat cgagactcgg tggaactctg
     3841 ggcgaggaat ctggattcaa aagcaactcc gaactccata tatttcttgt ccaaaggagc
     3901 cagttcctct tcaccaataa cggacgctaa cgcccgaatg tctttgacat gagaatacgc
     3961 cgcaaaaagc tgactcgcca ctggagcgtg atcgtcacgc gtcatcccag gcccaatccc
     4021 gtccttcatc agtctggaca aagacgggag acaggcaatg ggtggataca cacccttctg
     4081 atgcagttca tgatcgagaa cgatctgccc ttcggtgatg tatcccgaca aatccgatac
     4141 gggatgggaa atgtcatcgt tgggcattgt caacagtggc atctgagtga tggaacctgt
     4201 cttgcctttg atcataccag cacgttcgta aaggctcgcc aagtcactgt aaaggtaacc
     4261 tggataccct ttgcgactgg gaatctctcc acgaacgact gagacttcac gcaacgattc
     4321 gcagtagttg gtcatgtctg tcagaatgac aagcacatgc atatcggctt caaaagccag
     4381 gtactccgcc acggtcagag ctgcgcgtgg agtgatgatg cgctcaatgg gcggatcgtc
     4441 cgccagattc aggaacacca ccactttttc cataacgccg ctggactcga aactgtccat
     4501 aaaaaagcgc gccacgtcat gtttgatgcc catcgcccca aacactacag caaactgaag
     4561 gtcttccccc acgattctgg cctgtcgaac gatctgcgcc gccaaggtgt tgtgggcaat
     4621 accactacca gaaaagatgg gcagtttctg tccgcgaaca agcgtgttca gagcgtcgat
     4681 cgctgagatg cccgtatgga tatacttcct tggatagtcg cgtgccgtcg ggttgatggg
     4741 cagtccattg acatcccaac gtgccttggt gatggggggc ggtcccccgt cctgaggctg
     4801 acccaatccg ttgaagaccc tgccgagcat ctgttccgag gcggggattc gaagacactc
     4861 acctgtaaaa cgcgctttgg tgcggttcat ctggagtccg taggtaccgt caaaaacctg
     4921 aatgattgcg gcaccatcag atgcttccaa aacattaccc atccgaagcc tgccggaact
     4981 atcctcaact tcgacgagtt ctccccatcc gatgttgtgg atgcccttga tgacaaccaa
     5041 gggaccgtca accttctcaa gcccaacgta ctgaataccc gtctcagggg cagttgatgc
     5101 gctaggattc atagattgct cccaggcgct ccatggaacg ctccatctcg gtatctatct
     5161 tgtccaattc agccaactga tcgttggtaa tggtactttt catcttggca attttgggga
     5221 tgaccttgga cttgcgcagg ttcagatagg tcacgccctt ctggatgttg gcaacacctt
     5281 gacggtacag gttggtgatg attgacagca tcttcgcctg cttctgagca acacagaaca
     5341 tatccacctt gtcaaaggaa ttctgctgaa gaaaggcatt ccgaaaaatc tgacacactt
     5401 ccagaaccca acgctgggcg tcgggcagaa cgtccgctcc aacaagtt
//
LOCUS       DAOQUC010000055         5445 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_16526_length_5445_cov_3.914100, whole genome shotgun sequence.
ACCESSION   DAOQUC010000055 DAOQUC010000000
VERSION     DAOQUC010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5445
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16526_length_5445_cov_3.914100"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2234
                     /locus_tag="PKH07_01610"
     CDS             <1..2234
                     /locus_tag="PKH07_01610"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 20 zincin-like fold
                     domain-containing protein"
                     /protein_id="HNT33676.1"
                     /translation="WRVNFARNEIPHNEASSWAFVGPKFHTPSKFGGLIGMVVPSQYI
                     GTAGMLTPKFSSASATSAPVALDRAFSHQAASPDVIIPEPVLWEKTRESFRIDQDTLI
                     VIPEDSTQQDIWAADSLNREIQERGERPLKVVQAGDHFDQTGSMIVLGEPWRNRFIEP
                     ILKGTDLEVTKNAPGSEGYALVVQTDGVAASGSDPRGTFYAVQSMRQILRKDESGLYL
                     EGCRVYDWPRFAFRGVHLLLDKDSPEVHGKMIEDLFARYKVNAIVFEIEQGYQWESHP
                     EIASSRGCDRKGLEQLLDIAERNFIQTMPLVQTLGHLEFIFKAHPDLVEDPEAPYAYC
                     PLNPKSYQLMFDLLDEAIEVFRQPKYVHIGHDEFDMRGTFPAHEECRKLGKAALYEMD
                     TLKLYEYLQSKGVGTMMWGDILLEQHYTDMMRRLPKDILITDWHYAPAKEYPSVPFFT
                     GHRLDVLCCPWNTKTNITSFSQYASAHNAQGMLQTTWTGYFGNAAALQSLTSDLYCYI
                     LGAEYAWTPDKPELEETYVAANAFQHLWYDAARSHEVPGIAVDLFPFMNLSLKTDEQG
                     KGWLQLAPGHDLAGLPTGQVWLKDGIQYRLRETCAPDVAGAMMMCGNSWGEDFPSTVF
                     GIPVHGKSTALNFLHATVWTNDKDTRVGAYVVHYDDGSQVEIPLRQGKEVFGWLESGS
                     SPMTRIAWLGTTARGSKACLHSFRWINPSPDRKIETIDVVSDAPEGPLVVLAITAEVE
                     DSQ"
     gene            2231..3631
                     /locus_tag="PKH07_01615"
     CDS             2231..3631
                     /locus_tag="PKH07_01615"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HNT33677.1"
                     /translation="MNRVLRHTSLLSLSAAATKVLLIVFAVYVTNVLGLAALGRLEYY
                     LNLVFIFVVAADFGLEQWTTRELARRKEEGDALIPNLIALRLPFTAVALVLMALFLRF
                     ISRQQSVGFAGLIALTYLLSFSFQTLLRGMMRAYGMMGYESFLNFSERLLTVGAGVLL
                     IHAGYGAQALLGCFLVGNIAVLVATVFLLRGRGLWSWGRVQTAQWSGYVSGASLFGLA
                     AIFITIFYRQDTILLTELKGETATGGYRSAYRLVEGLWLVPQMLSVALYPVLSELFHK
                     GTKLDGICAQAFRLLTAISLPLAVGGTLVAPPLIRMIYPDNPDGIAIFAVLVWTMPFV
                     YGNFLVGTVLAATDRQRVNLIASIVAVLVNLGLNFFAIPRWLGLGAAVVSVVTQAAFF
                     LVMFVPMTRDMKNLGISGSLVRSLICCAAMAIAVGAVRPYGVLVQITLGALVYCLLAW
                     HLQLFSRDDLRQFRRA"
     gene            3797..4948
                     /locus_tag="PKH07_01620"
     CDS             3797..4948
                     /locus_tag="PKH07_01620"
                     /inference="COORDINATES: protein motif:HMM:NF025957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HNT33678.1"
                     /translation="MRAMIMFALGVVCSVVYAQSLPKGNAPADVLSASPELRSADHLP
                     ERGVEMTWMELSDPNLDMRGLPWYAENAPDLWRLPKRAQATTPQGAWNRALVPSGGRI
                     RLSCNTSCLSIRARMVAERNRPCFFDAYVGTELAGSAVISGTETVDLTLIEKTDHSMK
                     DITIYLPNTHHVRVDAIGVAADTQFRSPSSFALKRPLVCYGSSVLQGTGASRGAMTYP
                     ALLARQLNLDYVNLGFGGSGRAEPQVVSLVSEIDACGFLFDLGKSYGAQPIEVYAKML
                     DMIRASHPEVPIIVVTPIYSQRERTEPEYLDKSEKLRVLMRQAAQERIQAGDQRTFVV
                     EGLDLCGEADWDAFHDPTHPNDEGNRRMAERLAPLVEKVVLGRTSSAVR"
     gene            5139..>5445
                     /locus_tag="PKH07_01625"
     CDS             5139..>5445
                     /locus_tag="PKH07_01625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33679.1"
                     /translation="MKIGLLVCLIVGLMMGCSSESSKPRQQQASTGQAPQKPVEGTEP
                     ESAIEQKAEQPAASAPDASSASEQATVTKAGRRAVIEMEKGGEIEIEFFEDDAPNTVE
                     "
BASE COUNT         1156 a         1420 c         1548 g         1321 t
ORIGIN      
        1 tctggcgagt caactttgcc cgaaatgaaa ttccccacaa tgaggcgtct tcctgggcat
       61 tcgtcggacc caagtttcac acgccatcga agtttggcgg actgatcgga atggttgtgc
      121 cttcgcagta catcggcacc gccggaatgc tgacgccaaa gttttcttcg gcttctgcga
      181 cctcagcacc cgtcgcgtta gaccgggctt tcagtcatca ggctgccagc cctgacgtta
      241 tcattcccga accggtgctt tgggaaaaga ccagggaatc attccgcatc gatcaggaca
      301 cgctcatcgt catccccgag gacagcacac aacaggacat ctgggcggca gattcgctga
      361 atcgagaaat ccaagaacgg ggcgaaagac cgctgaaggt ggtgcaagca ggagatcatt
      421 ttgatcagac cggttccatg atcgtcctgg gtgaaccttg gcgcaacaga ttcatcgaac
      481 ccattctcaa gggtaccgat ctcgaagtca ccaagaatgc ccccggctcg gaaggctatg
      541 cgcttgtggt tcaaaccgat ggtgttgccg cgtcagggag tgacccacgc ggaacattct
      601 atgcggttca gtcaatgcga cagattcttc gtaaggacga atcgggtctc tatttggagg
      661 gatgtcgggt ctacgactgg cctcgattcg cctttcgcgg tgttcatctg ctcctggaca
      721 aggactcccc tgaagtgcac gggaagatga tcgaagatct ctttgcgcgc tacaaggtca
      781 acgccatcgt ttttgagatt gagcagggct accaatggga gagccatccg gagatcgcca
      841 gttccagagg atgtgaccga aagggactgg agcaactgct ggacattgcc gaacgcaact
      901 tcattcagac catgcctttg gtgcaaaccc tgggacactt ggagttcatc ttcaaagcac
      961 atcccgatct ggttgaagat cccgaggcac cttacgccta ctgtcctttg aatcccaaaa
     1021 gctaccaact catgttcgac ctgcttgacg aagccatcga ggttttccgt caacccaagt
     1081 atgttcacat cggtcatgat gaattcgaca tgagaggtac ttttccggcg catgaggaat
     1141 gccgcaaatt gggcaaggcg gcactgtatg aaatggatac gctcaaactc tatgaatacc
     1201 ttcagtccaa aggtgtcggc acgatgatgt ggggagacat tctcctggag caacactaca
     1261 ccgatatgat gaggcgattg cccaaggaca tcctgatcac cgactggcat tacgctccag
     1321 caaaagaata tcccagtgtg ccattcttta ccggtcatcg ccttgatgtt ctgtgctgtc
     1381 cctggaatac gaagaccaac atcacctcgt tcagccagta cgccagcgca cacaatgccc
     1441 aaggcatgtt gcagaccact tggacgggat acttcggaaa tgcggcggcg cttcagagtc
     1501 tgacttctga tctgtactgc tatatccttg gtgcagagta tgcctggacg cccgacaaac
     1561 cggaacttga agagacctac gttgccgcaa atgccttcca gcatctgtgg tacgatgcgg
     1621 cgcgcagtca cgaggtgcca ggtatcgcag tagacttgtt tcccttcatg aatctctccc
     1681 tgaaaacgga tgaacaaggc aagggatggc tgcagttggc accgggacac gatcttgccg
     1741 gactgccgac aggccaggtt tggctgaagg atggaatcca gtataggctc agggaaacat
     1801 gcgctccgga tgtcgctggg gcaatgatga tgtgtggaaa ctcatggggt gaggatttcc
     1861 caagcacggt atttgggatt ccagtccacg gcaaatctac ggcattgaac ttccttcatg
     1921 caaccgtctg gacgaatgat aaggacaccc gggtcggtgc gtatgtggtt cactatgacg
     1981 atggttcgca ggtggagatt cctcttcgtc aaggcaagga agtctttggc tggctggaga
     2041 gcggcagcag tccgatgacg cgcattgcct ggcttggaac cactgctcgt ggaagtaagg
     2101 catgtcttca ttcatttcga tggatcaacc cctctccgga taggaagatc gaaaccattg
     2161 acgtggtctc ggatgcgccg gaaggcccgc tggtcgttct ggcaatcacc gctgaggtag
     2221 aagacagcca atgaaccgcg tgctgcggca tacctcactt ctttctctgt ctgctgcggc
     2281 gaccaaggtc ttgctgatcg tctttgccgt ctatgtgacc aatgtcctcg gcttggctgc
     2341 gcttggtcgt ctggaatact acctcaatct tgtcttcatc ttcgttgtcg ctgctgactt
     2401 cggtctggag caatggacaa cgcgagaact ggcacgccgc aaggaagaag gcgacgccct
     2461 gattccaaat ctgattgcct tgcgcttgcc gtttaccgca gtcgctctgg tgcttatggc
     2521 gctctttctg cgcttcatct ccaggcagca gtcggtgggt tttgctggat tgatcgccct
     2581 gacgtacctt ctctcattct cctttcagac actgttgcgc gggatgatgc gtgcctatgg
     2641 gatgatggga tatgaatcgt ttctgaattt ctcggaacga cttctgaccg tgggtgccgg
     2701 tgtcttgctg attcacgctg gctacggtgc ccaggcgctt ctgggttgct tcctggtggg
     2761 caacatcgcc gtgctggtgg cgacggtctt ccttcttcgc ggccgaggcc tctggagttg
     2821 gggaagggtt caaacagcgc agtggtcagg ctatgtgtct ggggcttctt tgtttggact
     2881 ggcggcgatc ttcatcacca tcttctaccg gcaggacacg attctgctca ctgagttgaa
     2941 aggggagacg gccacagggg gataccgcag cgcctaccgt cttgttgaag gactctggtt
     3001 ggttccacag atgctctcgg tggcgctcta tccggtgctc tctgaacttt ttcacaaagg
     3061 cacaaagctc gatggaatct gtgcgcaggc gtttcgtttg ctcactgcaa tttccctgcc
     3121 ccttgctgtt gggggaaccc ttgttgcgcc accattgatt cgaatgatct atccggacaa
     3181 ccccgatgga atcgcgatct tcgctgtcct ggtctggaca atgccttttg tttacgggaa
     3241 ctttctcgtt ggcacggtct tggccgccac ggatcgacag cgcgtcaacc ttatcgccag
     3301 catcgttgcc gtgttggtca atctgggact caactttttt gcgattccga gatggctggg
     3361 cttgggcgct gcggtagtgt ccgttgtgac tcaggcggca ttcttccttg tgatgtttgt
     3421 gcccatgaca agggacatga agaatctggg catctccgga agtctcgtcc gatcgctgat
     3481 ctgttgtgcc gcgatggcga ttgctgttgg agccgttcga ccgtatgggg tcttggttca
     3541 gatcacgttg ggagcactgg tctactgcct cttggcatgg catctccaac ttttctcgcg
     3601 agatgatctt cggcaatttc ggcgcgcata gggagcgtgt cacaaggggg tgttgtccag
     3661 agaggagaga caggatgagt tggctgagtc ctatcgcacg agatagttct tactgatcgt
     3721 ggctggccta tattccccca gtcttctata ctgtctccat gtgccgctga tttctaaatg
     3781 caggaggtcg ttgccgatgc gagccatgat tatgtttgcc ctgggtgtcg tgtgcagtgt
     3841 ggtttatgct cagagcctac cgaaagggaa tgcccccgcc gacgtcctgt cggcttcgcc
     3901 ggaactcaga agtgccgacc atctgcctga aagaggtgtt gagatgacat ggatggaact
     3961 gtccgatccg aatctggata tgcgcggctt gccgtggtat gccgagaacg ctccagacct
     4021 ctggcgactt ccgaaacgtg cccaagcaac gactccgcag ggtgcctgga atcgggcttt
     4081 ggttcccagc ggtggtcgca tccgtttgtc ctgcaacaca tcctgtctga gcattcgtgc
     4141 ccgaatggtt gctgaacgca atcggccgtg ctttttcgat gcctatgtcg gcaccgaact
     4201 ggctggcagt gcggttatct ccggaacgga aacagttgac ctgacactca tagagaagac
     4261 cgatcactcc atgaaggaca tcacgatcta tcttcccaac acgcatcacg ttcgtgttga
     4321 cgccatcggc gtcgctgcgg atacccagtt tcgatctccg tcttccttcg cgctcaagcg
     4381 acctcttgtc tgctatggtt cttcagtttt gcagggcaca ggagcttctc gtggagccat
     4441 gacctatccg gcgctactgg cgcgtcagtt gaatctggac tatgtgaatc tcgggtttgg
     4501 gggttctgga cgagccgagc ctcaagtggt ctctctggtc agcgagatag acgcctgcgg
     4561 ttttcttttc gaccttggca agtcttacgg cgctcaaccg atcgaagtgt acgcgaaaat
     4621 gctcgacatg attcgggcgt cccatcctga agtgcccatc attgtcgtga cgccgatcta
     4681 ctcacagagg gagcggacgg aaccggagta tctcgacaag tcagagaaac tcagagttct
     4741 gatgagacag gcagcacagg agcgtatcca agcgggagat caaaggactt ttgtcgttga
     4801 agggctcgat ctctgtgggg aagcagactg ggatgcattc cacgatccga cacatccgaa
     4861 cgacgaaggc aatcggcgca tggccgaacg actcgcacct ctggtcgaaa aagtggtgtt
     4921 gggtagaacc agttctgcag tacgctgaat tcgacaagca gatttttctc ttcggttgcg
     4981 gttcgcacat cctgcctgag acacaggaat gggaatagag aaacgacaga atcggtctgg
     5041 tttgaacgca acgtaatgtt gtgtataatc cccttatgag tacttcccag gaatggataa
     5101 gcctgggttg aggagattct tcggaggtca tcaagagaat gaagattggt ttactggtat
     5161 gtctgattgt gggtctgatg atgggttgtt catctgagag ttccaagccc aggcaacagc
     5221 aggcaagtac agggcaagcc cctcagaagc ctgttgaggg gacggaaccc gaatcggcca
     5281 ttgagcaaaa ggcagagcag cctgcggcga gtgctccgga cgcgtccagc gcttccgagc
     5341 aggcgacggt caccaaggca ggacgccgcg cggtcattga gatggagaag ggtggagaga
     5401 ttgagatcga gtttttcgaa gacgatgcac ccaacactgt cgaga
//
LOCUS       DAOQUC010000056         5413 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_16644_length_5413_cov_3.484696, whole genome shotgun sequence.
ACCESSION   DAOQUC010000056 DAOQUC010000000
VERSION     DAOQUC010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5413
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16644_length_5413_cov_3.484696"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>676)
                     /locus_tag="PKH07_01630"
     CDS             complement(<1..>676)
                     /locus_tag="PKH07_01630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33680.1"
                     /translation="VWYNPIGETATPDQTVEVDLIDGSYSGNVTLTTSGVAFFRARWQ
                     GNPPTVQGAESPRVVLPVSASAAPELQQQWEFNPGVSVLVTGATSGNLAQPTRDYLAN
                     LGYYVLRHRRYNDMKIVYMNNQPVQDYDQDGTPDPIVDVIQSSVAALSLAFDQAVSQL
                     SPTAPLSVFVVGEKGTGSTILMGDGSQLAASEFNTLLNTKFGTTRDVRVFVEASQSGN
                     FCQAIAA"
     assembly_gap    677..686
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<687..4735)
                     /locus_tag="PKH07_01635"
     CDS             complement(<687..4735)
                     /locus_tag="PKH07_01635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HNT33681.1"
                     /translation="MGTEIVTHSFECSLEGFGIQNIALLTPQDRTFTIFRNDSQREGL
                     GVEVDFDIEDMSEDDANTNFPAGEYTLSVLFDDGRTDLLPLRLGTDYPSYPDIIYPQA
                     GVTDVPVNPTISWIAPDDSMGLLLEIDGLDCICSYEVPLVRTASSYQVPDGILDAGRS
                     YVLELVSYGENTQAIARIFFKTQGTPSNRYFPFSVQDSQYSVFVSKQNEMKRQVEVTF
                     QDDFSTYSKFSILTPNEIPFLLFSSPLAVNEFTLDYEVQIEWNEDPLSSHLAPATGIG
                     QYAFRFEDPNGSLVATRNVTTPLQFPETPNVLTPPCDASITVGAPIRWSVTDSSLINA
                     LGISFEYDSPQTWGSEYEALLSPQERVWSIPLFTDYADNDADSEIFALSLVVSSTDTF
                     GGLVSCAEHSYFLTHDPLPEIWEAEPNNDPSTANPIPDVSTTLAMISVDWDEDWYTFA
                     GVQGEEVTLEVFADRSGSAEDCSIEVWSGADSTHPFATGESTPAASRLELQDGRVDVI
                     YGKLVDFAPSTWRFFSLRASCVDPGLSLTLPETGRYFVRVKGHQRVHSDRYGRFYRIQ
                     LRREPKQWESANVVLTKPDSSTVVLELFEQGDYSEMLTPTTFDATFPSGDYLLTVDFP
                     DGTTIEYNYSSVDSFPTMPEFLKPTHQSLVTVPVDVQWTVQDADRIFLMMQEPVGGFS
                     HLAAYPKDVTETTVLGVPEHSNFHLSLSAEKIIPTEDMEDGTLRRQVVSNILFSTGAS
                     TEATPTPTHTPTAVPEIVVPSTDFPTIQSAIDAAADGTVIKVLPGTYFENINFHSKDI
                     ILRSSSPDDAEVVARTIIDGGAQAPTVTLGVLQPASCILEGFTIRNGGGSGGGGISAD
                     LSRATIRNNRIVDNVTDFGGGIRNAQGLIENNYIARNNADQGGGLDWCTGTIRNNTIV
                     DNKSNLGGAIHAASGLIERNFIARNDALNGGAAAECVGSFANNILVNNSAEYGAGLYL
                     CSGEIVNNTFFGNTATGYGGALFGCGGVVLNCIFWLNRDAFGQTIDDSTVPSYSCIDE
                     QIVGGVGNLFQEPIFVNPSMDDFHLSEFSPCIDRGVAHPSITQDFEGDRRPIEGTSEP
                     RAPYAKFDMGADEFDPRSLRPDFAVQVQNPRVVVQSGERPVFRVNLLATNGFDEEVTL
                     SVPNASFTHAFSPNPALPPCSTTLLLERASDRATDTTTEFTIRASGKSGSLVREVSAS
                     FSVVGRDSTVPRLSLWQWPTRIRLGATVFLRGTVLPAEPSASINVSVSRRDQDGNQTA
                     LPSVQATTDAQGNFRLDYVPDQLGTYQFTASLVRDNAVRSSTVSVDVYAKSSSISLKV
                     SPQTMPVIGSSIPFSGRLFPFVAGEPAKVT"
     gene            complement(4903..>5413)
                     /locus_tag="PKH07_01640"
     CDS             complement(4903..>5413)
                     /locus_tag="PKH07_01640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33682.1"
                     /translation="WITEMGLSTEGGVSEQDQAQYLPKAMLTAASQRNVERVYWFNLT
                     DWGPGGRFGLCRQDGTPKPSFLAFRQLLIAESGSRHQVMPELSSGVVVHKFSSPKARK
                     QTLVLWSRKGTQKVSVRMVGLDALLTVQDYLGREVLRSKKVGESIRLTVGESPLYLFS
                     KDPILVESL"
BASE COUNT         1302 a         1403 c         1455 g         1243 t
ORIGIN      
        1 gctgcgatgg cttggcagaa attccccgac tgggacgcct caacaaagac acgaacatcc
       61 cttgtggtgc cgaacttggt attgagaagt gtgttgaact cagaggcggc caactggctt
      121 ccatcaccca tcaggatggt gctgcccgtt cccttttctc ccacgacaaa gaccgaaagc
      181 ggcgccgttg gtgacaactg gctgacagcc tggtcaaaag ccagtgaaag agcagcaaca
      241 gaagactgga tgacatccac gatgggatca ggcgtgccat cctgatcgta gtcctgcaca
      301 ggttggttgt tcatgtaaac gatcttcatg tcattgtagc ggcggtgtcg caggacgtag
      361 tatccgaggt ttgccagata atctcttgtc ggctgtgcga gatttcccga ggtcgctccg
      421 gtgacaagta ccgacactcc tggattgaac tcccattgtt gttggagttc tggtgctgct
      481 gacgccgaaa caggcaacac aacgcgaggg ctctccgctc cctgaaccgt tggcggattg
      541 ccctgccaac gggctctgaa gaaagcgacg ccactcgtgg tcagcgtgac attgcctgaa
      601 taagaaccgt cgatcagatc cacttccaca gtctgatccg gagtcgctgt ctcccctatc
      661 ggattgtacc agacatnnnn nnnnnngtca ccttggcagg ttcccccgcc acgaagggaa
      721 acaggcggcc ggaaaacgga atgctggaac cgatgacggg catagtctga ggactcacct
      781 tgagtgaaat cgaagaactc tttgcataca cgtccactga aacggtggaa ctccgcaccg
      841 cgttgtcccg aaccagtgat gccgtgaact ggtatgttcc caactggtca ggaacataat
      901 caagacggaa gttcccctga gcgtccgttg tcgcctgaac actgggaagt gccgtttggt
      961 tgccgtcctg atctcggcgc gatacagaaa cattgattga agcggaaggc tcagccggaa
     1021 gcactgtgcc gcgcagaaac accgttgcac ccaagcgaat ccttgtcggc cactgccaaa
     1081 gactgagtcg aggtactgtg ctgtctctcc cgacaacgga gaacgatgcc gaaacctctc
     1141 ggacaaggga accggattta ccgctggcac gaatcgtgaa ctccgtggtt gtatcagtcg
     1201 ctctgtcgga agccctctcc agaagaagcg ttgtggagca tgggggcaga gcgggattcg
     1261 ggctgaatgc atgagtgaaa gaggcatttg gaaccgagag tgtcacttcc tcatcaaacc
     1321 cgtttgtcgc cagcagattg acacggaaca cagggcgctc gccgctttgg acaacgaccc
     1381 tgggattctg tacctgaaca gcaaagtcgg gacgcagtga gcgtggatca aattcatcag
     1441 cacccatgtc aaacttggcg taaggtgccc gaggctccga cgtgccttct atcggtcttc
     1501 tatcaccctc aaagtcctgc gtgatgctcg gatgagccac gcctctgtca atacagggag
     1561 agaactctga aagatgaaag tcgtccatgc tggggttgac gaagatcggc tcctggaaga
     1621 gatttccgac gcccccgaca atctgttcat caatacagga ataagatggg acggtggaat
     1681 catcaattgt ctgaccgaac gcatcgcgat tgagccagaa gatacaattc aagacaactc
     1741 ctccacagcc gaagagagca cctccgtaac ctgtcgctgt gttgccgaag aacgtgttgt
     1801 tgacgatttc cccactgcac aaatacaagc ccgctccgta ctcggcgcta ttgttgacaa
     1861 ggatattgtt tgcaaaagag ccaacgcact cggcggcagc ccctccattc aaggcgtcat
     1921 tgcgggcaat aaagtttcgt tcgatgaggc cggaagcagc gtggatcgct cctccgagat
     1981 tgctcttgtt gtcaacaatg gtgttgttgc gaattgtccc cgtacaccag tccaggccac
     2041 ctccctggtc tgcgttgttt ctggcaatgt agttgttttc gataagacct tgagcgttgc
     2101 gtatcccccc accgaagtct gtgacgttgt ccacgatgcg attgtttcga atggtggcgc
     2161 gagacaaatc cgctgaaata cctcctccac cggaaccgcc tccattgcga atggtaaatc
     2221 cttccagaat gcacgacgcg ggttgcagaa cacccagagt gacggtgggt gcttgtgctc
     2281 cgccatcaat gatcgttctc gcaaccactt cagcatcgtc gggactgctt gagcgtagga
     2341 tgatgtcctt actgtggaaa ttgatgtttt cgaaataggt tccggggaga accttgatca
     2401 ccgtgccgtc ggctgctgcg tcaattgccg actgaattgt cggaaagtca gtactgggca
     2461 caacgatctc cggaacagcg gttggtgtgt gggttggtgt gggtgtggcc tctgtcgaag
     2521 cacctgttga gaacaggata ttgctgacaa cctgacggcg cagagtacca tcttccatat
     2581 cttctgtcgg tatgattttt tcagcactca gcgacagatg aaagttcgag tgctcaggaa
     2641 ctcccagtac ggtggtttct gtgacgtctt tggggtaggc tgccagatgg ctgaacccac
     2701 caacaggttc ctgcatcatc aggaagatgc ggtctgcgtc ctggaccgtc cactgcacat
     2761 caacgggcac cgttacgagg ctttggtgag tgggtttgag aaactcaggc atcgtgggaa
     2821 atgaatctac agatgagtaa ttgtattcaa tcgtcgttcc atcgggaaaa tccacagtga
     2881 gaagataatc accagagggg aacgttgcgt cgaaagtggt tggagtcaac atctccgaat
     2941 agtccccttg ttcaaacaac tccagaacca cggttgagga atctggcttg gtgaggacaa
     3001 cgtttgcgct ctcccactgc ttgggctctc ttctaagttg gatccgatag aaccttccat
     3061 atctgtccga gtgcactcgc tggtggcctt tgactcggac aaagtaccgt cctgtctcgg
     3121 gaagcgtcag cgaaagacct ggatcaacgc aggaggcgcg caatgagaag aaacgccacg
     3181 tactgggtgc aaaatccacc agtttgccat agatgacgtc cactcgtccg tcttgcagct
     3241 cgagtctgct cgccgccggt gtgctctcac cggttgcaaa ggggtgtgtg ctgtcagcgc
     3301 cagaccagac ctcaatagag cagtcttcag cggatcctga tcgatctgcg aaaacttcga
     3361 gggtgacctc ctcaccctga actcctgcaa aagtgtacca atcttcgtcc cagtctacgg
     3421 agatcatggc cagtgtcgtc gagacatcgg ggatcgggtt tgccgtactt gggtcgttat
     3481 tgggctctgc ttcccaaatc tcaggcagcg ggtcatgcgt aagaaaatag ctgtgttccg
     3541 cgcatgagac gagcccgcca aaggtgtctg ttgaagagac aacgagcgac agggcaaata
     3601 tctcagaatc agcgtcgttg tccgcatagt ccgtgaaaag gggaatgctc cagactctct
     3661 cctgcggaga cagcagtgcc tcatactcag agccccatgt ttggggagag tcatactcaa
     3721 acgaaatccc cagcgcattg atcaggctac tgtcggtaac actccagcgt atgggtgcac
     3781 cgaccgttat actggcgtcg catggcggag tgagaacgtt tggagtctcc ggaaactgaa
     3841 gtggagtagt gacatttcgg gtggcaacaa ggctgccgtt tgggtcttcg aaacgaaaag
     3901 catactgtcc tatgcctgtg gcaggggcta aatgtgaaga caatggatct tcattccatt
     3961 cgatttgaac ctcatagtcg agagtgaact cgttgacagc aagcggagag gaaaacagca
     4021 ggaagggaat ttcgtttgga gtcagaatgg aaaactttga ataggtgctg aagtcgtcct
     4081 gaaacgtcac ttccacttgt cgtttcatct cattctgttt gctcacaaag acggagtact
     4141 gcgaatcttg aacagaaaag ggaaagtacc tattgctggg cgtaccttgt gtcttgaaaa
     4201 agatccgcgc tatggcttga gtgttctctc cgtaggaaac cagttccagg acgtaactcc
     4261 gacccgcatc aaggatgcca tcaggaacct gataactgct tgcagtgcga acgagaggaa
     4321 cttcatagga gcagatgcag tccagcccat cgatctccag cagcaatccc atcgaatcat
     4381 caggtgcaat ccagctgatg gttgggttga caggcacgtc agtcacgcct gcctgtggat
     4441 agataatgtc gggatagctc ggataatccg ttccaagacg cagagggagc aaatccgttc
     4501 tgccgtcgtc aaaaagaaca gaaagcgtgt actcgccggc tgggaaattg gtgttggcgt
     4561 catcttcgct catgtcctca atatcgaaat ccacttccac gcccaggcct tccctctgtg
     4621 agtcatttct gaagatggta aaagtcctgt cttggggagt gaggagagcg atgttctgaa
     4681 ttccaaagcc ttcaagactg cactcaaaac tgtgagtcac gatttccgtt cccatatcca
     4741 ttgtgcgagc gcggctaata tgggacccgt ccagatctat ttgctgtgct gaaacagtat
     4801 ctacggcggc gcagcatgcc agaaccataa gaacacaagc aaatgcttga tgggatctta
     4861 gactcgtcat cgtgtatctc ctgaactctt gatataaatg tgctacagac tctcaaccag
     4921 aatcggatct ttgctgaaga ggtacagggg gctttctcca acagtcaggc gaatggactc
     4981 tccaaccttc ttggagcgca agacttcgcg acccaagtag tcctgaacgg tcagcaatgc
     5041 gtccagtccc accattcgaa cagagacttt ctgagttccc tttcgcgacc acaggacaag
     5101 tgtctgtttt ctggctttgg gggaggagaa tttatgcact acgacgccag aagacaactc
     5161 tggcatcact tgatgccggg atccagattc ggcaatgagt agttgccgaa aggcgaggaa
     5221 agaaggtttc ggtgttccat cttgccgaca cagaccgaat cgtccaccag gtccccagtc
     5281 agtcaaattg aaccagtaga ctcgttcaac atttctctgg gatgccgcgg tcagcattgc
     5341 tttgggaaga tactgtgcct gatcctgttc tgagacacca ccttcggtgg acagccccat
     5401 ctctgtaatc cat
//
LOCUS       DAOQUC010000057         5403 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_16692_length_5403_cov_3.225318, whole genome shotgun sequence.
ACCESSION   DAOQUC010000057 DAOQUC010000000
VERSION     DAOQUC010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5403
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16692_length_5403_cov_3.225318"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..664
                     /locus_tag="PKH07_01645"
     CDS             <1..664
                     /locus_tag="PKH07_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33683.1"
                     /translation="NQKQILPNEDYIVDTSRQELAKRVSTELGLVLTPAFDKLADEMG
                     GQKKAFGEVKEELKKRLTDLMTQTRAENAELREALRTNELLLRRSYEEGRAADTNIQQ
                     GIDKTLTLQQKDYAEEKMTNEQLRQELRRTRALQEQTYERLRTMSERYLATLKGQNAL
                     AQTGGAVKDIVTVGIPRKKFLAEEQKYVEEQLKAIEEERASIDGKELAPSTSGTPQDA
                     TP"
     gene            complement(932..1987)
                     /locus_tag="PKH07_01650"
     CDS             complement(932..1987)
                     /locus_tag="PKH07_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013146811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter ATP-binding protein"
                     /protein_id="HNT33684.1"
                     /translation="MGIEVRNITKRFGEFTALDNVSLRVPKGDLVALLGPSGCGKTTL
                     LRIIAGLENADEGRVLFDGEDATHHRVQERRVGFVFQHYALFRHMTVFENVAFGLRVK
                     PKRFRPSEQEIKDRVQELLGLVHLEWLADQYPSQLSGGQRQRVALSRALAVEPKVLLL
                     DEPFGALDAQVRKSLRRWLRRLHDEIHVTSVFVTHDQEEALEVADRVVIMKDGTVVQS
                     GTPVEVYEQPATAFVYKFLGNVNHFPGTISEGKAHVGNMAFDLPAEAQGQTASIYVRP
                     HEMILDHSPFGDRCFRASVRHINPAGPYVKVELITDWGESVDVEISREEYQRLNLTRD
                     AEVYASPRETKIYLEQG"
     gene            complement(1977..2879)
                     /gene="cysW"
                     /locus_tag="PKH07_01655"
     CDS             complement(1977..2879)
                     /gene="cysW"
                     /locus_tag="PKH07_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HNT33685.1"
                     /translation="MKDRKAKDIRIPIAQSAITEPPVIRYALIAVALSFLVFFLILPL
                     VTVFSQALSKGLRAYLVVFADSDTISAIRLTLITVAVAVPLNTLFGIIAAWAIAKFEF
                     RGKMVLITLIDLPFSVSPVISGLVFILIFGSQGLLGPWLSAHHLKIVYAVPGIALVTT
                     FVTLPFVARELIPLMQEQGTEEEEAALVLGATGLQTFFRVTLPNIKWGLLYGVILCNA
                     RAMGEFGAVSVVSGHIRGLTNTVPLHVEILYNEYNFVGAFAVASLLAFLALVTLALKS
                     VVEWKSERSGRRMTGVREEATDGH"
     gene            complement(2902..3735)
                     /gene="cysT"
                     /locus_tag="PKH07_01660"
     CDS             complement(2902..3735)
                     /gene="cysT"
                     /locus_tag="PKH07_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011142071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HNT33686.1"
                     /translation="MSRIKKNSVIPGFGLTMGLAMTYLCLLVLIPLSFLFVKTATLGW
                     REIGQIISSPRVIASLRLTFLTSLIAAAINAIFGLLVAWVLVRYEVFGKRFIDALVDL
                     PFALPTAVAGISLTAIYSKNGWMGSLLEPLGIKVAFTPLGITVALTFIGLPFVVRTVQ
                     PVLQDLAAEVEEASACLGATRWQTFWKVVFPCLQPAVLTGFTLAFARALGEYGSVVFI
                     SGNMPMRTEIAPLLIIAKLEQFDFAGAATLAAMMLVASFVLLLLINIIQWKTAQRFSE
                     Q"
     gene            complement(3823..4800)
                     /locus_tag="PKH07_01665"
     CDS             complement(3823..4800)
                     /locus_tag="PKH07_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HNT33687.1"
                     /translation="MFVGVMLGQVALAQAEFLNASYDPTRELYQEFNQAFGAYWKEKT
                     GVQLTLRQSHGGSGSQARAVIGGLEADVVTLALAYDIDAIVEKAKLLPENWQTLLPDN
                     SSPYVSTVVFLVRKGNPKGIKDWDDLVKPGIEVITPNPKTSGGARWNYLAAWGHASEK
                     FGGDKEKITDFMKALFKNVPVLDSAARGATTTFVQRGIGDVFLAWENEALLALREIGV
                     EKVELVIPSVSILAEPSVAIVTENAKKHGTTEIAEAYLRFLYSETGQDIAARHYYRPR
                     LQSVADKYKEQFPDIKMFTIDQTFGGWKKAQQEHFSDGGTFDQIYQPGS"
     gene            complement(5048..>5403)
                     /locus_tag="PKH07_01670"
     CDS             complement(5048..>5403)
                     /locus_tag="PKH07_01670"
                     /inference="COORDINATES: protein motif:HMM:NF014950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNT33688.1"
                     /translation="VLEMMQRYPWPGNIRELQNVVECLAILCDSDRIEVEHLPMPFHS
                     GNASTMAVPATWEEMKPLLHEAREQAERDMIQRFLEQALRASGNNISLAARKTGLHRT
                     TFHALMKKCGVAHKK"
BASE COUNT         1343 a         1465 c         1400 g         1195 t
ORIGIN      
        1 taaccagaag cagattctcc ccaatgaaga ctatatcgtt gacaccagcc ggcaggaact
       61 tgccaagaga gtctccacag aactcggcct tgtcctgacg ccggcattcg acaaactggc
      121 cgacgagatg ggggggcaga agaaggcttt tggtgaggtt aaagaggaac tcaagaaacg
      181 tctgacggac ctgatgactc agacaagggc ggaaaacgcc gaactgcgtg aggcactgag
      241 aaccaatgaa ctgctgcttc gtcgttccta tgaagagggt agagcagcgg acacaaatat
      301 ccagcagggc attgacaaga cgctgaccct tcagcagaag gattacgccg aagagaaaat
      361 gaccaatgag caactgcgtc aggaactgcg ccgcacccgt gcgctacagg agcagaccta
      421 tgagcgcctt cgcaccatgt cagaacgcta cctagcgacg ctcaaaggtc aaaatgcatt
      481 ggcacagacg ggtggtgcgg taaaggatat tgtgaccgtt ggcattcctc gaaagaagtt
      541 tcttgcagag gaacagaaat atgtcgagga acaactgaag gccattgaag aggagcgtgc
      601 ctcgatagat ggcaaggaat tggcgccatc cacatccggt acccctcaag acgcgacacc
      661 ttagagaatc ctggtcttct cacaatagac gaaaggggac aaagacaact gacaggtttc
      721 caactgtgtc agagtccctt gggattaccg aattcccgct cagcagttga gaaagatgaa
      781 tgtcccgatg gatattcggc attaccaagc gtgaatgcat tctgaatgcc ttggtatctc
      841 ggcgatggct ggcgctctgc cccgattttt gcttggagtt gaactgtccc gcagttcggt
      901 tctgaaaccg ctgatgccca ctcgctaatt gtcacccttg ttcgaggtat atcttggttt
      961 ctcttggaga ggcgtagacc tcagcgtctc gggtaagatt caaacgctgg tattcctcac
     1021 gcgaaatctc gacatccacc gattctcccc agtcggtgat cagctccacc ttgacatagg
     1081 ggcccgctgg attgatgtgc cgaactgagg ctcgaaagca tctgtctccg aagggcgagt
     1141 gatcgaggat catctcgtgc ggtctgacat agatgcttgc tgtctgtcct tgtgcctctg
     1201 ctgggagatc gaaagccata ttcccgacgt gcgcctttcc ctccgagatc gtgccgggga
     1261 agtgattgac attgccaagg aatttgtaga caaaagccgt tgccggttgt tcatagactt
     1321 cgaccggtgt tccactctgt accacggtgc cgtctttcat gatcacgacg cggtcggcga
     1381 cctcgagcgc ctcttcctgg tcatgcgtga cgaagacact ggtgacgtgg atctcatcat
     1441 gcaaacgtcg caaccaacgc cgaaggctct ttcgaacctg ggcatccaac gcgccgaagg
     1501 gttcatccag aagaagcacc tttggctcga ccgccaaagc gcgtgacaat gccacacgct
     1561 gtcgctgtcc accggacaac tgtgaaggat actgatccgc aagccattcg agatgaacca
     1621 gccccaagag ttcctgcacg cggtctttga tctcctgctc cgagggtcgg aatcttttgg
     1681 gcttgactct gagaccaaag gccacattct caaacacggt catgtgtcga aacagggcgt
     1741 agtgctgaaa tacaaaaccg actcgacgct cttgaacgcg gtgatgtgtt gcgtcttcac
     1801 catcgaacag cacacggcct tcgtcagcgt tctccagtcc ggcgataatt cgcaacaacg
     1861 tggtcttccc acaacctgac ggacccagca gagcgaccag atcgcccttg ggtacccgga
     1921 ggcttacgtt atccaatgca gtgaactcac caaatctctt tgtgatgttg cgaacttcaa
     1981 tgcccatcgg ttgcttcctc tcgaactcct gtcatgcgcc gtccacttcg ttctgacttc
     2041 cactcgacga ccgacttcag agccagtgtc accaaagcca aaaaagccag cagcgaagcc
     2101 actgcaaaag cgccgacgaa gttgtactcg ttgtaaagaa tctccacatg cagaggaacg
     2161 gtgttggtca gtccccggat atgtcccgac acaaccgaga cggcaccgaa ctcacccatc
     2221 gctcgcgcgt tgcagaggat gacgccgtac aagagtcccc atttgatatt tggcagggtc
     2281 acacggaaaa aggtctgcag tcccgtggca cccagaacaa gtgcggcctc ttcctcttcg
     2341 gtcccttgct cttgcatcaa gggaatgagt tcccgagcga caaaaggaag tgtcacaaac
     2401 gtggtgacca atgcgatgcc tggtaccgcg tagacgatct tgagatgatg tgcggaaagc
     2461 caggggccga gcagcccctg tgatccgaat atgaggatga agacaagtcc cgagatgacc
     2521 ggcgagacag agaagggaag gtcaatgagt gtgatcaaga ccatcttccc gcgaaactcg
     2581 aacttggcga tcgcccaagc ggcaatgatt ccgaacaacg tgtttagagg cacggcaacc
     2641 gccacggtaa tcagtgtgag tcgaatggcg gagatcgtgt ccgaatctgc aaaaacgacc
     2701 agataagctc gcaacccctt ggaaagagcc tgtgagaata cggttaccag cggaaggatg
     2761 agaaagaaga caagaaaaga aagtgccacc gcaatcagtg cataccgaat cacaggcggc
     2821 tctgttattg ccgactgcgc gataggaatc ctgatgtcct ttgcctttct gtccttcaag
     2881 tcttcctgcc ttgatcggat ctcattgttc gctgaagcgc tgtgcagtct tccactggat
     2941 gatgttgatg agcaatagaa gcacaaagga tgcgaccaac atcatcgctg ccagcgtggc
     3001 agcgcccgcg aagtcaaact gctccagttt cgctatgatg agaagcggtg ctatttctgt
     3061 tctcatgggc atgtttccgg agataaaaac caccgaaccg tattcaccca gagctcgcgc
     3121 gaatgcaagt gtgaaccctg tcagaactgc gggttgaaga caggggaaga cgactttcca
     3181 aaacgtttgc cagcgcgttg cacccaagca cgcggatgct tcttcgactt ctgccgccag
     3241 atcctgaagt actggttgaa cggttctgac gacaaacggc agtccaatga aagtcagtgc
     3301 aaccgtgatg cccagaggcg tgaaagccac cttgatgccc aacggttcaa gcaggcttcc
     3361 catccagccg ttcttcgaat agatggcggt caatgagata ccggcaactg ctgtcggcag
     3421 tgcgaaaggc agatccacaa gggcgtcaat gaatctcttt ccaaacactt cgtagcgtac
     3481 cagaacccag gcaacaagca gtccgaatat ggcgttgata gcagccgcaa tgagggatgt
     3541 gaggaaggtc agtcgcaatg aagcaatcac gcgcggcgaa ctgattatct gtccaatctc
     3601 ccgccagccg agcgttgctg tcttcacaaa gagaaaagac aagggaatga gaaccaggag
     3661 acacagataa gtcatcgcca gacccatcgt caagccgaat cctgggatga cactgttctt
     3721 cttgatcctg ctcatcgagc gctccaaaga tccttgagga cggacacagg atttgattct
     3781 tcctgtgtcc gtcccgcttc gaaaaccatg aaacaagaac cgctaactgc cgggctggta
     3841 aatctggtca aaagttcccc catcgctaaa gtgttcctgt tgtgctttct tccatcctcc
     3901 gaaagtctgg tcaattgtga acatcttgat gtcggggaac tgctccttgt acttatcggc
     3961 aaccgactga agccggggtc tgtagtagtg acgagcggca atatcctgtc ccgtttctga
     4021 gtacaggaac cgaagatacg cttcggcaat ctcagtcgta ccatgcttct tggcattctc
     4081 agtgacaata gcgacagacg gttctgccag gatgctgacg ctcggaatca ccagttcgac
     4141 tttttccacg ccgatctcac gcaaggccaa tagcgcctcg ttctcccaag cgagaaacac
     4201 atcgccgatt ccgcgctgca caaatgtcgt tgtcgcgcca cgagctgctg agtccagcac
     4261 gggaacattc ttgaacagtg ccttcatgaa gtcggtgatt ttctctttgt caccgccaaa
     4321 cttctctgag gcgtgtcccc aggcggcaag gtagttccag cgcgccccgc ccgaggtctt
     4381 cgggttggga gtgattacct caattccagg tttcaccaaa tcgtcccagt ctttgattcc
     4441 cttggggttt cctttgcgca ccagaaagac aactgtggaa acatagggtg agctgttgtc
     4501 gggaagcaag gtctgccagt tctccggaag cagttttgcc ttttcaacaa tggcatcaat
     4561 gtcataggcg agggcaaggg tgacaacgtc agcttcgaga ccaccgatga cggcacgagc
     4621 ttgcgaaccg gaaccaccgt gcgactgtcg aagtgtcagt tggacaccag tcttttcctt
     4681 ccagtatgca ccaaaagcct ggttaaactc ctggtagagt tctctcgtcg gatcatagga
     4741 agcgttcaga aactccgcct gcgccagagc gacctgacca agcatcactc cgacaaacat
     4801 cgcaacgaac acactgactg ccgaacctct cttcatgaat tctgtacctc attgtctgtt
     4861 ttgcagagcc aaactctatt gtatgtgtcg cagtcgttgt gccactttct cgactaagaa
     4921 agacaatgct acatttcact gatatagttg atgttatgga tcaaaggctg aatctccagg
     4981 cgctggaggg gcgtgaatct gtagttaatg tatcaacact gttgctgttt tgtagacaca
     5041 agagtgccta tttcttgtgg gcaactccgc acttcttcat caacgcgtgg aaggtggttc
     5101 gatgaagtcc tgtcttgcgg gcagcgagac tgatgttgtt gccagaggca cgcagcgcct
     5161 gttccaggaa gcgttggatc atatcccgct ctgcttgttc ccgcgcctca tggagcagtg
     5221 gcttcatctc ctcccaagtt gcaggaactg ccatcgtcga agcatttcca gagtgaaacg
     5281 gcatcggaag gtgctcaact tcgatacgat cgctgtcaca gagtatagcc agacactcca
     5341 ccacattctg aagttcccga atgttgccag gccacggata gcgctgcatc atttccagca
     5401 ctt
//
LOCUS       DAOQUC010000058         5378 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_16806_length_5378_cov_3.531843, whole genome shotgun sequence.
ACCESSION   DAOQUC010000058 DAOQUC010000000
VERSION     DAOQUC010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5378
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16806_length_5378_cov_3.531843"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..630
                     /locus_tag="PKH07_01675"
     CDS             <1..630
                     /locus_tag="PKH07_01675"
                     /inference="COORDINATES: protein motif:HMM:NF017828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HNT33689.1"
                     /translation="LLGRLREAVFTAPVCPMPALEMLIAEMLVIHFCSDRRATRAVLQ
                     GLVDEVEHRISNGAGVLPNDAARIFWVNPTADWRMMNLLEQCGGRLCGSEFLFCHALD
                     LIAEEMDPMEALARSALADPMVGSAADRAERIRRDIVRFGAEGVVISRIPGASHCATE
                     GHLIKEYLFRTMDLPIVELESPPIIDAMQATLRTRLEALIETAKERRRS"
     gene            627..1406
                     /locus_tag="PKH07_01680"
     CDS             627..1406
                     /locus_tag="PKH07_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015050077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HNT33690.1"
                     /translation="MICAGMDAGSRTVKLILWDSERQRVIGRGRVEQGTDQNRRAHDL
                     FREVLANSGLSASDVRRTIATGYGRHLIDFADTTVTEITCHARGVRFLIPDVRTVIEI
                     GGQDSKVIKMTPEGTVRDFAMNDRCAAGTGRFLEMTAQRLEMTLDDLACLSTKSVNPA
                     VISSMCVVFAETEIVGLLAQGTACEDIVAGVQRSIAERLLSMLGNKVDDPVVFTGGVA
                     TIPSMVRALQDVLERSVTTAPEPFYTGALGAALLASVDKTQ"
     gene            complement(1456..2574)
                     /locus_tag="PKH07_01685"
     CDS             complement(1456..2574)
                     /locus_tag="PKH07_01685"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HNT33691.1"
                     /translation="MNVLAFSVVGLLFVFVSVRCHGLEYGINNGAPISASTGAISPAN
                     DGQILMDTGTEWVRVNFILGPWSSPGDATPRGPQNLSWFETYDQIIDGMLAKGLKVYG
                     LIGAEAVPNSRRDRLNTEEYAQEYAAVFQQIVDHFKGRIKVYESFNEPNDWAGGTVSQ
                     VEPRWFARYLELIYRAVKMDGERKNDPVLQEIVLVSGPLFTHLNDLGREYFSATWKAG
                     TTQWGWDEVKSQTGSYPLDGVGIHIYVAQGEESDEELVALMKRNLDAMYQSLTELEGA
                     DVSKRFYLSEFGWTTKHVSEQMQAHKMDVAFRLLELDSRMAQAHWFTLQDFPDGEYGI
                     YRAGALTPENRKPSYEMFRSHATGLNRARPPMNAILCR"
     gene            complement(2834..3889)
                     /gene="pspF"
                     /locus_tag="PKH07_01690"
     CDS             complement(2834..3889)
                     /gene="pspF"
                     /locus_tag="PKH07_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008101439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HNT33692.1"
                     /translation="MTRTHEEPLQGRSSGTEVKEAIGQSEAFLAFQEKLSRVASVNRP
                     VLIIGERGTGKELAAARLHYLSPRWQGPLVTLNCAALAPSLIESELFGHEAGAFTGAG
                     SRRTGRFEAAHGGTLFLDEIGTIPLQAQEKILRVVEYGSFERVGSSMAVNVDVRIVGA
                     TNADLAGLAKQGRFKKDLLDRLSFEVLVLPPLRERQEDILLLANHFATRMAFELGWKE
                     IPEFSEQAIRQMEQHSWPGNVRDLKNVVERAVYRSDSALINEITFDPFAGTYSVTAQD
                     QPESQPMKQTNKPAETEDLASMAETLRQTELSYLKKALEQSRFNQKKAAGLLGLSYHQ
                     FRWLLRKHKDNINVLTQ"
     gene            4087..4767
                     /gene="pspA"
                     /locus_tag="PKH07_01695"
     CDS             4087..4767
                     /gene="pspA"
                     /locus_tag="PKH07_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006963445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HNT33693.1"
                     /translation="MGIFSRMRDIISSNINAMLDKAEDPEKLVRLMIQEMEDTLVEIK
                     ASCAGTIANRKKMERFLEEAKDRATGWAEKARTAVNRGFEDLAREALCQKRRFMERSQ
                     SLEQELDKCQTLIECYQNDMMRIEEKLAAAREKQRLLIQRHIHAQTHKRAEEEIRRLD
                     TSDAFVRFEKFETRIDRMEADADLVNFGRKPTLEEQFSKIETDDEIERELAQLKSTGT
                     KKEPKADA"
     gene            4902..5165
                     /locus_tag="PKH07_01700"
     CDS             4902..5165
                     /locus_tag="PKH07_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005986029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33694.1"
                     /translation="MAGFRLGVAELAVILIFGIPLVAILGSFAIAALKILRGSSSRQM
                     SAEESRLVQEIHNSLQRMEQRVDALETILLERDRWSDREGARR"
     gene            5162..>5378
                     /locus_tag="PKH07_01705"
     CDS             5162..>5378
                     /locus_tag="PKH07_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020887040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HNT33695.1"
                     /translation="MTKISFAVLLLPVLAFVALLLVGFILVVVGLFRRGGSGRQMSGE
                     EVLLIQEMHQGFQRMEQRVDALETILSE"
BASE COUNT         1240 a         1358 c         1503 g         1277 t
ORIGIN      
        1 cttctcggcc gcttgagaga ggccgtcttc acagctcccg tctgtccgat gcccgcgttg
       61 gagatgctga tcgccgagat gcttgtgatc catttctgtt ctgatcggcg ggcaacgcga
      121 gcggttctgc aaggtcttgt ggatgaggtg gaacatcgaa tcagcaatgg cgctggtgtg
      181 ctcccaaatg acgcggctcg gattttctgg gttaatccca ccgccgattg gcggatgatg
      241 aatctactgg agcagtgcgg tggacggtta tgtgggagcg aatttctctt ttgtcatgcg
      301 ttggatctga ttgcggaaga gatggatccg atggaggctc tggcgcgatc tgcactggct
      361 gatcccatgg ttggttcggc tgccgatcgt gcagaacgca ttcggcgtga tattgtacgc
      421 tttggtgccg aaggggttgt gatctctcgg ataccgggcg cgagtcattg tgccaccgag
      481 gggcatctca tcaaggagta ccttttccgc acgatggatc tccccatcgt ggagctggag
      541 tcaccaccta tcattgacgc gatgcaggca acacttcgaa cccgtcttga ggcgcttatc
      601 gaaacagcca aggagagacg acgatcatga tttgtgcagg gatggacgca ggttctcgaa
      661 cggtgaaact gattctctgg gattcagaga ggcagagggt cattggccgc ggccgcgttg
      721 aacagggaac agatcaaaac cgaagggctc acgatctttt cagagaggtt cttgccaact
      781 cggggctgtc tgcttctgac gttcgccgaa ccatcgccac gggttacgga cgccatctca
      841 ttgatttcgc tgacacgact gttaccgaga tcacatgtca cgcgcgaggg gttcgatttc
      901 tgatacccga tgttcgaacc gtgatcgaga tcggaggcca ggacagcaaa gtgatcaaga
      961 tgactccgga aggaacggtt cgggatttcg caatgaacga ccgatgtgcg gcaggaacgg
     1021 gtcgctttct cgagatgacc gcacagcggc tggagatgac gttggacgac ctggcgtgtc
     1081 tctccaccaa gagtgtcaat ccggctgtca tcagcagtat gtgtgtcgtc tttgcagaaa
     1141 ccgagattgt gggactcttg gcacagggaa ctgcttgcga ggatattgtc gcgggagtgc
     1201 aacgttccat agcggaacga ctgctctcca tgttaggcaa caaggtggac gatcctgttg
     1261 tttttacagg tggtgtggca acaattccgt ccatggtgcg ggcgctgcag gatgtgttgg
     1321 aacgttctgt gacaacagcg ccggagccct tctacacagg cgctttgggc gcagcgcttc
     1381 tggcgtcagt agacaaaaca cagtgaagag agcctttccc cacttcagca caacaccagc
     1441 cggaagaaga attgatcagc ggcacaggat cgcgttcatc ggtggtcggg cacggttcag
     1501 tcctgtcgca tgacttcgga acatttcata ggacggtttc cggttttcag gggtgagtgc
     1561 tcccgctcgg tagattccgt actctccatc ggggaagtcc tgaagtgtga accagtgtgc
     1621 ctgtgccatc ctgctgtcga gttcgagcag tcgaaaggca acatccatct tgtgagcctg
     1681 catctgttcg gaaacatgct tcgtcgtcca tccaaactcc gatagataga agcgtttact
     1741 gacatccgcc ccttcgagtt ctgtcaggct ctggtacatc gcatcgagat tccgtttcat
     1801 tagagcgaca agttcctcgt ctgattcttc accttgagct acgtagatat ggataccgac
     1861 cccatccaga ggataggaac ctgtctgcga cttcacttca tcccaacccc attgggtcgt
     1921 gccagccttc catgtagcgc tgaagtactc cctgcccaga tcattcagat gtgtgaacaa
     1981 gggaccgctg acgagcacga tttcctgcag cacaggatcg ttctttcgtt ccccatccat
     2041 cttgacggca cggtagatga gttcgaggta tcgggcaaac catcggggct cgacctgact
     2101 gacggtgcca cctgcccagt cgtttggttc gttgaaagat tcatatactt tgattcgacc
     2161 tttgaaatga tccacaatct gttggaaaac agcagcatac tcctgagcat attcctccgt
     2221 gttcaagcga tcccgtctgg agttgggaac ggcttccgct ccaatgagtc catatacttt
     2281 cagtcccttt gccaacatcc catcaatgat ctgatcatag gtttcaaacc aggaaagatt
     2341 ctgagggcct cgcggtgttg cgtcacccgg agaactccat gggcccagaa tgaagttcac
     2401 tcgcacccat tcagtgccgg tatccatgag aatctgcccg tcatttgcag gactgatcgc
     2461 gcctgtactc gcactaatgg gtgctccgtt gttgatgccg tactccaagc cgtgacaacg
     2521 aacactaacg aaaacaaaca acaaacccac gacagagaat gcaaggacgt tcatcagcaa
     2581 acctcttctc gcttttggtg tctctcagac tctcatcttg tcaccctcaa cttagatgag
     2641 gctcgcttgg aagacagagg gtggagagtc tgagagaaac gggcttttcg cgcaccagga
     2701 gttcagagac aggaagcctc tccgtactct tccaagacaa gggagactac gccatttcat
     2761 attcgagagc aagtcggggg aatcatcgag tggtcaggaa ccctgagact tctgataact
     2821 gtattccatt ctgttactga gtcaggacgt tgatgttgtc tttgtgtttg cgcaaaagcc
     2881 atctgaattg gtggtaactc aaacccagaa ggcctgcggc tttcttctgg ttgaatcggg
     2941 actgctccag cgccttcttc agataggaca attcggtctg tctgagcgtt tctgccatcg
     3001 aggcaaggtc ttcagtttca gccggtttgt ttgtctgctt cattggctgt gactcaggtt
     3061 gatcctgtgc cgtgacacta tacgttcccg cgaaggggtc gaaagtgatt tcattgatga
     3121 gggcagaatc cgaacgatag acagcacgtt caacgacgtt tttcagatcg cgcacgttgc
     3181 ctggccacga atgctgctcc atctgtcgga tggcttgttc gctgaactcg ggaatctcct
     3241 tccagcccag ttcgaaagcc attcgtgtcg caaaatggtt ggcgagcagg aggatgtctt
     3301 cctgtcgctc gcgcagagga ggcagcacga gcacttcgaa actcaaccgg tccaagagat
     3361 ctttcttaaa gcgcccctgc ttggcaagtc cagccaggtc cgcatttgtc gctccgacga
     3421 ttcggacatc cacgttgacc gccatcgagc ttcccacccg ttcaaaggaa ccgtactcaa
     3481 caacgcgcag aatcttttcc tgagcctgga gaggaattgt gccaatctcg tcgagaaaca
     3541 gcgttccccc atgagcggct tcgaaacgtc ccgtgcgcct tgagcccgca ccggtgaaag
     3601 cacctgcctc atgtccaaat agctccgact caatgaggct tggagccaga gccgcgcagt
     3661 tgagcgtgac caacggacct tgccaacggg gagagagata gtgcagtcga gccgcggcaa
     3721 gttccttgcc ggttccacgc tctccgatga tgagcactgg tcggttgacc gaagcgactc
     3781 gggagagctt ctcttgaaat gcgagaaacg cttcagactg gcctattgct tccttgactt
     3841 ccgtgccgga ggatcgacct tgaagtggtt cttcatgcgt tctggtcata tctttgatga
     3901 atttttacac ctattaatag catttaacgc tatcatataa tatattatat catagaaaaa
     3961 aagcaagcaa aatcacttaa ctcaaatgaa ctgagcaaat taactaattt gactagaatt
     4021 ggcatcctgc ttgctttgaa tgtctaatgt ttttgagcgt atatctgaat gtcggaggtt
     4081 actcaaatgg gaatcttctc acgaatgcga gacatcatca gttccaatat caacgcaatg
     4141 ttggacaaag ccgaggatcc ggagaaactc gtccgcctca tgattcagga gatggaggac
     4201 acgctggtgg aaatcaaggc atcctgcgcc ggaactattg ccaaccgaaa gaagatggag
     4261 cgcttcctgg aggaagccaa agaccgggca acgggttggg cggagaaggc gcgtaccgcg
     4321 gtcaaccgtg ggtttgagga tctcgcccga gaggccctgt gtcagaagcg gcgtttcatg
     4381 gagcgaagcc agtctctgga gcaggagttg gacaagtgcc agactttgat tgagtgctac
     4441 cagaatgata tgatgcggat tgaagagaaa ctggcagccg cgcgagagaa gcagaggctg
     4501 ttgattcagc gtcacattca cgcccagacg cacaagcgag cggaagagga gatcaggcgt
     4561 ctggatacca gcgacgcatt cgtccgattc gagaaattcg agacgcgcat tgatcggatg
     4621 gaagccgacg cggacttggt caactttggg cgcaagccga ctctggaaga gcagttttcc
     4681 aagatcgaga cggatgacga gatcgaacgt gagttggctc agttgaaatc cacaggaaca
     4741 aagaaggaac ccaaagcgga cgcataactg ctgaaaggtg acaatacccc gagacagatc
     4801 tcgaccctct ccgaagaccg gggagggtcg agaggggaaa aagggaactc ctccgcctca
     4861 gcggagacga taagaaactc tggatcaagg acctaagaat tatggctggt tttcgtcttg
     4921 gtgttgctga gcttgcggtg atcctgatct ttggtattcc tttggtggcc attctgggaa
     4981 gctttgccat cgcggcgctg aagattctga ggggtagttc cagccgacag atgtctgccg
     5041 aggagagtcg tctcgttcag gagattcaca acagtctgca gcgcatggag cagcgagtgg
     5101 atgctctgga gaccatcctt ctggaacgag atcgctggag cgatagagag ggtgcacgac
     5161 gatgacgaaa atcagtttcg cggttctatt gcttcctgtg cttgcttttg tggcgctctt
     5221 gctcgtaggc ttcatccttg ttgtcgtagg gctgttccgc agaggcggtt caggtcggca
     5281 gatgtctggt gaagaagtgc ttttgattca ggagatgcac caaggctttc agcggatgga
     5341 gcagcgcgta gacgctctgg agaccatcct ttccgaaa
//
LOCUS       DAOQUC010000059         5344 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_16942_length_5344_cov_2.885801, whole genome shotgun sequence.
ACCESSION   DAOQUC010000059 DAOQUC010000000
VERSION     DAOQUC010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5344
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16942_length_5344_cov_2.885801"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..406)
                     /locus_tag="PKH07_01710"
     CDS             complement(<1..406)
                     /locus_tag="PKH07_01710"
                     /inference="COORDINATES: protein motif:HMM:NF017508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor family protein"
                     /protein_id="HNT33696.1"
                     /translation="MVTIAKTDQLTETKKVLHVEVPRDKVEEKVVENFAKVQSETALP
                     GFRKGKAPIELVRLRFDKGVREQSVQDIINDVVRDILKEQNIRIVGIPKIDGIEKEPG
                     ENKDQPLKFTAEFEYIPSYTLVTDYSSIVVPEP"
     gene            complement(503..587)
                     /locus_tag="PKH07_01715"
     tRNA            complement(503..587)
                     /locus_tag="PKH07_01715"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(551..553),aa:Leu,seq:tag)
     gene            complement(643..715)
                     /locus_tag="PKH07_01720"
     tRNA            complement(643..715)
                     /locus_tag="PKH07_01720"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(680..682),aa:Lys,seq:ctt)
     gene            complement(984..1060)
                     /locus_tag="PKH07_01725"
     tRNA            complement(984..1060)
                     /locus_tag="PKH07_01725"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1024..1026),aa:Arg,seq:tct)
     gene            complement(1082..1157)
                     /locus_tag="PKH07_01730"
     tRNA            complement(1082..1157)
                     /locus_tag="PKH07_01730"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1122..1124),aa:Pro,seq:tgg)
     gene            complement(1317..2897)
                     /locus_tag="PKH07_01735"
     CDS             complement(1317..2897)
                     /locus_tag="PKH07_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011753219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar isomerase"
                     /protein_id="HNT33697.1"
                     /translation="MALKTVHPCRQHCLCVPHPEHSCTSVTRREFIAGMGTLALGGCA
                     LSTLRAGEKLVTPNSRQVHPMIATLKVRPVLVFEIPQRREAVSWRSWGGIQTREHVVQ
                     EKERISKELVRLLACAEFPIELLNLEEVETPEQAARVASGDHDVLLIYAAGNHAGVLD
                     ALVRRDKWNLMYLRHNTGPVYLWYEVAHPIFLRKTVDQFAQTGLQTRDVVVDDYTEIL
                     WRLRSLHGLKKSLGRRIVAIGGSGGWGAGGAEAPNLAREVWQMDIQDVSYEQLGKSIA
                     KGRSNAALVASCQREAKDYLSQRGVTLRTSLDAVVNAFVLKEVFLDILEEYQCDAITV
                     QWCMGTIMPLAETTACLPLSLLNDEGYLAFCESDFVVIPSGILLQGICGMPVFLNDPT
                     YPHDNQITLAHCTAPRKMNGRKVERAIILTHFESDYGAAPKVEMKIGQKVTNLIPDFS
                     ARNWVGCSGTILANPFLDICRSQIDVKLDGNCDELVAEMKGFHWMTSYGNYLKEIGYA
                     LEKVGVKWNNISGEESAS"
     gene            complement(2982..4178)
                     /locus_tag="PKH07_01740"
     CDS             complement(2982..4178)
                     /locus_tag="PKH07_01740"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNT33698.1"
                     /translation="MMKTYFATPERASASEIKSDLIKVCESEFVKCLLDVASGLVAVL
                     NIHRQILLVNDAYLEALGIDDPDSVLGLRPGESLGCIHATEMPAGCGTSKFCQSCGAA
                     VAIVTSKESPTPVEELCTVCIKRDGASQELCFRVKACRLRIGSSSFILVFIQDVTSVQ
                     QWAAMERIFLHDIGNLVTALEGASEILRFADEQRMPLLAHRIRQMATRLAHEISLQKI
                     LLHKEFGEYELTVETFMLSELLQEARTVINEHPCSRNRSWTMEDPVADVQFSTDFSLV
                     LRVLINMLTNAFEATDEGGTIRSWLEPHTDSVTFCLWNQKPIPSPLAIRVFQRHFTTH
                     REVGRGLGTFSMKLLGEQLLGGKVSFTSSEKEGTVFRFQIPLSSQGDSHSSQSRQITE
                     DFPANQ"
     gene            4379..>5344
                     /locus_tag="PKH07_01745"
     CDS             4379..>5344
                     /locus_tag="PKH07_01745"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HNT33699.1"
                     /translation="MSIVVRLTLNGREVQAQEGETILSVAGRSGISIPTLCFLEGRSR
                     LESCGVCVVEVEGQSSLVPSCAFRVTDAMVVRTDTPTVVEARRKALELLLSDHVGDCC
                     APCELACPAGIDIPGFIRLIVRSQFQEALAVIKRRTAFPGILGRICPRYCEKACRRGQ
                     YDESVSICALKRFPTDWCKAEGESVPVSAAPSGKTVCIIGGGFAGLSVAWFLMREGHQ
                     CHIYERETVLGGAVQRYIPEFRLPADGVTCEIDALIAMGLQTHMGSEIATAAQLNELS
                     AQYDVVCLATGAPCETRAGFPGEDLCLKSRAYLSALKMETVSDLRD"
BASE COUNT         1265 a         1385 c         1372 g         1322 t
ORIGIN      
        1 ggggttcagg gacaacgatc gatgaataat ctgtgaccaa tgtgtaggac ggaatatact
       61 cgaactcagc cgtgaacttc agcggctgat ccttgttttc cccaggttct ttctcaatgc
      121 catcaatttt tggaatgcca acaatgcgga tattctgctc tttgaggata tctcttacga
      181 catcattgat aatgtcttgc accgattgtt cccggacacc cttgtcaaaa cgaagtcgaa
      241 ccagttcgat gggcgctttg cctttgcgga acccgggaag ggcagtctct gactgcactt
      301 tggcgaagtt ctcgacgacc ttttcttcaa ccttgtcgcg aggcacttcc acatgaagga
      361 ctttctttgt ctccgtcaac tgatctgttt ttgctatagt aaccatctgt ctccacccaa
      421 atcttcgata ttccgaacca cacacactca aagcgcatcc atcggttctg agaaatggat
      481 gccaagcgac tcgactccgc tctggtgcga gaggcgggag ttgaacccgc atgagtttcc
      541 tcactggatc ctaagtccag cgcgtctgcc aattccgcca ctctcgcttg tttggcgcct
      601 acacagtcaa gcggaagcgg aaagtcgggt caaaaaatat agtgagccgt ggaggaatcg
      661 aacctccaac ccactgatta agagtcagtt gctctgccag ttgagctaac ggcccaccga
      721 ttcgaatttc cgtcctccta cgatgattac aggccggcac gtcaacgatt tcgttggcac
      781 cagccttgat aaccgtagag gattattcca tcatgcgatc tctttgtcaa tagagaccgc
      841 gtgataaaac ttgtcaaaga acgactcgca tgtctttgtt ggatgttcag caggctcaag
      901 cctgcgtgtg cgatatagat tccaagaatc ggcgtcctgg agtccgtgtt cctgtttccg
      961 acaccaagat ttatgagcac acttggcgcg cctgagagga ctcgaacctc tggcccgctg
     1021 cttagaaggc agctgctcta tccacctgag ctacaggcgc acaatcacaa caaagatatt
     1081 ttggtcgggg tgactggact tgaaccagcg acctcctgct cccaaagcag gcgcgctacc
     1141 aactgcgcca caccccgagg tgcctccagt gaagcgcaaa gaatctaact cagtgacaga
     1201 tcgaatgcaa gcattttcgg gagtgtgagc cctcaacagt catgttcttg atatctcgtt
     1261 ggcagagggt aaggagaata tctgaacaca agagcaaggg tttactggct cctggctcac
     1321 gaagcggact cttcaccgct gatgttattc cacttcactc caaccttctc gagggcatat
     1381 ccaatctctt tcagatagtt gccatagcta gtcatccaat ggaatccctt catctcagca
     1441 accaactcat cacagttgcc atcgagtttg acatcgatct ggctgcggca gatgtcaagg
     1501 aacgggtttg caaggatcgt gccagaacaa cccacccaat tgcgagcaga aaaatcaggg
     1561 atcagattgg tgaccttctg tccgatcttc atctcgacct taggtgcagc gccgtagtca
     1621 gactcaaagt gtgtcaggat gatagccctt tccacctttc ttccattcat ttttctgggg
     1681 gctgtgcagt gtgccaaggt gatttggttg tcgtgtggat aggtcggatc attcaggaag
     1741 acgggcatgc cacagatgcc ttgcaggagg atcccagatg gaatgaccac gaaatccgat
     1801 tcacagaacg ccaggtaccc ttcgtcattt agaagactca aagggagaca ggcagtggtt
     1861 tccgccaagg gcatgatggt tcccatgcac cactgaacgg tgattgcatc acattggtac
     1921 tcttcaagaa tatcgagaaa cacttccttg aggacaaatg cattgacgac agcatccaga
     1981 gaagtcctga gcgtcactcc tctttggctg aggtagtctt ttgcctcgcg ctgacatgag
     2041 gcaacaagcg ctgcgttgct tcgccccttc gcgatagact ttcccaactg ctcgtaagag
     2101 acgtcctgaa tatccatctg ccaaacttct cgggcgaggt tcggtgcttc cgctccacct
     2161 gctccccagc cgccggaacc accgatggcg acaatccttc tgccaaggga tttcttgaga
     2221 ccgtgaagcg agcgaagtcg ccagaggatt tctgtgtagt cgtccacaac aacgtctctg
     2281 gtctgcagac ccgtctgagc gaactgatcc acagtctttc gaagaaaaat ggggtgagcg
     2341 acctcatacc acagatagac aggtcccgtg ttgtgacgca gatacatcag attccatttg
     2401 tccctccgaa ccagtgcgtc aagaacacct gcatgatttc ctgcggcata tatgagaagg
     2461 acatcatggt cgcctgacgc cactcgggca gcttgttcgg gcgtctcgac ctcttcgaga
     2521 ttgagcagtt cgattggaaa ctctgcacac gcaaggagcc tgacaagttc cttggagatt
     2581 ctctcttttt cctgaacaac gtgttcacgt gtctgaattc caccccagga tcgccaactt
     2641 accgcctcac gacgctgggg aatctcgaaa acaagaacag ggcgaacctt aagcgtcgca
     2701 atcattggat gcacttgcct ggagtttggc gtgacaagtt tctcacccgc ccgcaacgtg
     2761 gacagggcac acccgccaag agccagggtt cccatccccg cgatgaactc ccgccgtgtg
     2821 accgaagtgc atgagtgttc gggatgaggc acacagaggc aatgctgtct acatggatga
     2881 accgttttta gggccatagt attgaaagat tcctttctta ttcttgttgt caagacagaa
     2941 cactgaaaag atcactcaaa cacgtattct ttcttgaaga actactggtt tgcggggaaa
     3001 tcctccgtaa tctgccgtga ttggctggaa tgcgaatccc cctgtgatga caggggaatt
     3061 tgaaaccgaa agactgttcc ttccttctca gaacttgtga aggatacttt tccccccaag
     3121 agttgttctc ccaaaagctt catggagaat gtaccaagtc cacgtccaac ctctcgatga
     3181 gtcgtgaagt ggcgttgaaa gactctgatc gccagaggac tgggtatcgg tttctgattc
     3241 cagagacaga aggtaacgga gtccgtatga ggctcaagcc atgatcggat cgttcctcct
     3301 tcatccgttg cttcaaaggc atttgtcagc atattgatca gtaccctcaa gacgagggag
     3361 aaatcagttg agaattggac atcagccacg gggtcttcca ttgtccagga gcggttcctg
     3421 gagcagggat gttcgttgat gactgttctc gcctcctgaa gcagttcact cagcatgaag
     3481 gtctcaactg tcagttcata ctctccgaac tccttgtgaa gaagtatctt ctgaagggat
     3541 atctcatgtg caagcctggt tgccatctga cggattctgt gagccaggag aggcattcgt
     3601 tgctcatctg caaatcgcag aatctcagaa gcgccctcaa gggcggtcac aagatttccg
     3661 atgtcgtgca ggaaaattcg ttccatggct gcccattgtt gaactgaggt tacatcctgg
     3721 atgaagacaa ggatgaatga cgacgatcca atgcgcaggc gacaggcttt gacgcgaaaa
     3781 catagttctt gtgatgctcc atcgcgtttg atgcagacgg tgcagagttc ctcaaccgga
     3841 gtcgggctct ctttcgaggt gacgattgct acagcagctc cgcaggattg acagaacttc
     3901 gaggtaccac agccagcggg catctccgtt gcgtgaatgc atcccagcga ttctcctgga
     3961 cgaagaccca agacagagtc tgggtcatca attcccagtg cttccaagta ggcatcattg
     4021 accaggagaa tctggcggtg tatgttcagg acggcgacga ggccactggc tacatcgaga
     4081 agacatttca cgaattcact ctcgcagacc ttaattaagt cagacttgat ctcggaagca
     4141 gatgctcttt cgggtgtggc aaagtatgtc ttcatcatgg ttgggcgtct ccttcgcccc
     4201 gcttcatccc ggcctggaat ccctgaacaa gcctctctga aaaccctgat tgtgatatcc
     4261 gaaagctacc ctaatcagat taccaaaatg cttaacagag agcaatcaca gtttgccaaa
     4321 tgtttgagtt gatcccagat ggatattctg cggataatcc tgccggagga agtgtacggt
     4381 gagcattgtt gtacggctga cgctcaatgg tagggaagtt caggctcaag aaggcgaaac
     4441 catactctct gtggcaggcc gctctggtat ctccattcct acactctgct ttctggaagg
     4501 cagaagtcgc cttgagtcct gtggcgtatg cgttgttgag gtggaaggcc agtcgagtct
     4561 cgttccatct tgtgcctttc gggttactga cgccatggtt gtcaggacag acactccaac
     4621 cgttgtcgaa gcgcggcgga aggctcttga actactgctg tctgatcatg tcggggactg
     4681 ttgtgccccg tgtgagttag cctgccctgc ggggatagat atccctgggt tcatccgcct
     4741 gatcgttcgg tcgcagttcc aggaagcttt ggcagtcatc aaacgccgga ctgccttccc
     4801 gggaatactg ggtcgaatat gccccaggta ctgcgagaag gcctgtcgaa gaggtcagta
     4861 tgatgaaagt gtttcgattt gtgctctgaa gagatttcca acagactggt gcaaagccga
     4921 gggggagagt gtccctgtgt ccgctgctcc ttccgggaaa acggtctgca ttatcggtgg
     4981 tggatttgct gggctttctg tagcgtggtt tctgatgcga gagggtcatc agtgtcacat
     5041 ctacgagcgt gagactgttc tgggaggggc agtacagcgg tacattcctg agttccggtt
     5101 gccagccgat ggggtcacct gtgaaattga cgctctcatc gcgatgggac tgcaaactca
     5161 tatgggaagc gaaatagcca cggcagcgca actcaatgag ctgagcgccc aatatgatgt
     5221 cgtatgtctt gccacaggag caccatgcga aactcgtgct ggttttccag gcgaggactt
     5281 atgtctcaag tcacgtgctt acttgtcagc gttgaagatg gagactgttt ccgacctgag
     5341 agac
//
LOCUS       DAOQUC010000060         5322 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_17027_length_5322_cov_3.051642, whole genome shotgun sequence.
ACCESSION   DAOQUC010000060 DAOQUC010000000
VERSION     DAOQUC010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5322
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17027_length_5322_cov_3.051642"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1071
                     /locus_tag="PKH07_01750"
     CDS             <1..1071
                     /locus_tag="PKH07_01750"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HNT33700.1"
                     /translation="VDPSNPVLPISHPESYAKEIQSSVGFYYTQGMPEDTKALSNKVF
                     TDEEFMQQSRFVMEERLRMYDFELGRFKQGLLFFYFTSLDQNSHMLWHALDESHPIHE
                     KDKASICCQYMEFLYHSMDEMLGKTLKTIDDNTTLIIMSDHGFASFRRCFQINTWLKE
                     NGYITLHDDFKQEEYEFFGNVDWSRTKAYNLGINSVYLNLRGREQKGTVDPSQKDALL
                     KEISEKLLAVRDPENGEQVITKVYKTSEVYRGAYVDIAPDLVLGFNRGYRASWETILG
                     GFPLGIVNDNEDKWSGDHCMASDLVPGVILSNKKIEIPDPTLADIGPTVLQEYGIEPS
                     KEMEGRALFDTNAAPAAIKPVV"
     gene            1121..1315
                     /locus_tag="PKH07_01755"
     CDS             1121..1315
                     /locus_tag="PKH07_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33701.1"
                     /translation="MFFGSGIKLDKALLEKVRKYSEIAGYSSVEEFITNALEKEIAQL
                     EEADSEEDIKKKLQGLGYIS"
     gene            1483..2310
                     /locus_tag="PKH07_01760"
     CDS             1483..2310
                     /locus_tag="PKH07_01760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33702.1"
                     /translation="MWMLSHGLTKFFDVLLIPFASLPALVGLAWISLLTGVGMLMVYR
                     HISDQDKIKHVKNRIKAHFLEIRLFKEDMVIGLRAQKNILINNFKYMGLALKPMLFML
                     PPIILVLVQLEVRYGYRSFQPGDTSVLSVRLASDPTQLGTIDLKVPEGIEIQTPAVRT
                     VESGHYGVDWRIGVTQRGNHDVSVQIGDQTYSKRLCAISRTTAMAPQVGASSFKEAIF
                     QPVERPFDPKGQVQSISIQYPPADMRLLGIHVNWLVGFFVLSIIFGFMLKGVFKVEI"
     gene            2550..4298
                     /locus_tag="PKH07_01765"
     CDS             2550..4298
                     /locus_tag="PKH07_01765"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNT33703.1"
                     /translation="MNKRMPFFTLLLVILSASVLLGCNREKPLVIPDLQDALATYDEN
                     TITADDLVMMCSTRSHRMEILAKSVSETNWAETRLTELQQERILSERAREQGLDQDPE
                     FVSARDNALADTVIRLYLRDKVDRKITLTEKDFVKYYEFNKQKYTSPLRFRFNQVWID
                     VKKWGQEEGRKRAQSALARLQAGEGSVPVLSEFSDREGEDRFKPLGDYKKGEIPYTEL
                     EEAVLSTPKGSFSDVVETPLGFHIVVDPEITPESIMSLEEARPEVRELLFEEKQKEKQ
                     EALYESLAKRINPVIRVDLIEKTDVNPAEVVLKVGEKKVTSKDFKYLLSKSPKKMTPR
                     EVLQGIAERMMLYEAAIQEKYAETKSFKKAFSFIEAKMLSDFYLNKVASDQPDPTDED
                     VEKFYSDFPHLFYREKEIEPSEIVIKAAIRPDMTRYEKLQAMQDAEQRIRDARRQILD
                     GLPFSIAALMSSESETRSQGGKMTRRTWGTGPKFDNVAFFLDEGQISEPVEMDDGYQL
                     IWVRASHDRFLPSFDEVREDALARLKRTRYKEKRATLLEETSQASPLKVDAELLGKWK
                     AWLEQTAKDVYLWSKI"
     gene            4382..>5322
                     /locus_tag="PKH07_01770"
     CDS             4382..>5322
                     /locus_tag="PKH07_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HNT33704.1"
                     /translation="MYGRAFSSAVLGIDAYLMEVEVDLSPGMPAFMIVGLPDTTVKES
                     RDRVKAALTNSGFYFPAKKITINLAPADVPKEGSGLDLPMAIAILAASGQVVSQNLEH
                     YVFLGELALDGQVRPVRGVLPIAAQMRKDKIERLIVPQANASEAAVVKGINVHGVETL
                     VEVVEALNGQRELAIHQVNADAAFEKASHYEVDMSDVKGQEHAKRALEVAAAGGHNLL
                     MIGPPGSGKTMLARRLPTVLPKMTFEEALETSKIWSIAGKMNPGEALIARRPFRSPHH
                     TISDAGLVGGGRSPMPGEVSMTHNGVLFLDELPEFNRS"
BASE COUNT         1351 a         1312 c         1498 g         1161 t
ORIGIN      
        1 gtcgatccca gcaaccctgt gctcccgatc agccacccgg aatcttacgc caaggagatc
       61 cagagttctg tcggtttcta ctacacccag ggaatgccgg aagacaccaa agcgctttcg
      121 aataaagtct ttacggatga ggaattcatg cagcagtcgc gtttcgtcat ggaagaacgg
      181 ctgcggatgt atgatttcga gttgggacgg ttcaaacaag ggctgttgtt cttttacttc
      241 acgagccttg atcagaactc tcacatgctc tggcacgctc tggacgaaag ccacccgata
      301 catgagaaag acaaagcatc catttgctgc caatacatgg aatttctcta ccacagcatg
      361 gatgaaatgc ttggcaagac gctgaagaca atagacgaca acaccacact gatcatcatg
      421 tccgatcatg gatttgcgtc attccgacgc tgtttccaga tcaacacgtg gctgaaggaa
      481 aacggctaca tcacactgca cgatgatttc aaacaggaag aatatgagtt cttcgggaat
      541 gtggattggt cgcgaacgaa agcctacaac cttggaatca acagcgtcta tctcaacctg
      601 agaggcagag aacagaaagg gaccgttgac ccgtcccaga aggatgctct gctgaaggag
      661 atctccgaga aactccttgc tgtgcgtgat cccgaaaatg gcgagcaagt catcaccaag
      721 gtctacaaaa cttcggaagt gtaccgaggc gcctacgtgg atattgcccc tgacctggtg
      781 cttgggttca acaggggcta tcgcgcctca tgggagacaa ttctcggtgg gttcccactc
      841 ggaatcgtga atgacaacga ggacaagtgg agcggagatc actgcatggc aagcgatctg
      901 gttcccggtg tcatcctcag caacaagaag atcgagattc ccgatccaac gttggcagat
      961 atcggcccaa cagtgcttca ggaatacgga atcgaaccgt ccaaggaaat ggaaggacgg
     1021 gcattgttcg acaccaacgc cgcgcctgcg gcgatcaagc cggttgtgta gtggacggat
     1081 gtccaatgca tccaaacgat ggagacaggg agtcaaagcg atgttttttg gttcagggat
     1141 aaaactcgat aaggcactgc tggaaaaggt gcgaaagtac tcggagatcg caggctattc
     1201 ctccgttgag gagttcatca caaacgcttt ggagaaagag atcgcccagc tggaagaggc
     1261 cgattccgaa gaagacataa agaagaaact tcaaggactc ggatacattt cctagcaggt
     1321 gagcacctgt gggcatttgg gctatgccca ctcttggtgt tgtgtgcgtg caaagggaac
     1381 cgctgcttcc gaagtctggt ttccgaagag aacggatgag ccggaacact ggcaagtcct
     1441 tttggtgcca cacggcaaga actgacttga ggatgaaaga gaatgtggat gttgagtcac
     1501 gggctgacca aattctttga tgtgttgttg attccgtttg cgtccctgcc agcactcgtc
     1561 ggactggcgt ggatatcgct cctgacggga gttggaatgc tgatggtcta ccgtcacatc
     1621 tccgatcagg acaagatcaa acacgtcaag aaccgcatca aagcgcactt tcttgaaatc
     1681 cgtcttttta aggaagacat ggtcatcggt ctgagggcgc agaagaacat ccttatcaac
     1741 aacttcaagt atatgggact tgccctgaag ccaatgctgt tcatgctgcc gccgatcatt
     1801 ctggtactcg ttcagttgga agtccggtac ggctaccgat ctttccagcc cggggatacg
     1861 agcgttcttt ctgtgcgact cgcttcggat cccactcaac tgggcacaat cgatctgaag
     1921 gttcctgagg gcattgaaat ccagactccg gcggtcagaa ccgttgaaag cggccactat
     1981 ggcgtggact ggagaatcgg cgtcacgcaa cggggaaatc atgacgtgtc ggttcagatc
     2041 ggtgaccaga catactcgaa aaggctctgt gccatcagtc ggacgacggc aatggcacca
     2101 caagtgggcg catccagttt caaggaagcc attttccaac cggttgagag accttttgat
     2161 cccaagggac aagtacagtc catcagcatt cagtatccac ctgccgacat gaggctcttg
     2221 ggcatacatg tgaattggct ggtcggtttc tttgtgctct cgatcatatt tgggttcatg
     2281 ctcaagggcg tgttcaaagt ggagatttga ccccggcatg agcggcagat atgcctgttt
     2341 cgcattggac tctccgcgga tgtagcctgc aagacttttc cattggtcgt cctgccttgc
     2401 acgatttgtg tctgttgggt atgatatttc agattgtcag gaaaccgact tctggttctc
     2461 agtcgtttgt gtatttggga caaggagatg cctgaggatt ccccaggctc acgtgcagtc
     2521 gcgttgactg gtcagagagg acttttggga tgaacaagcg tatgcctttt ttcactcttc
     2581 ttttggtgat tctctcggca tctgtgttgc tgggatgcaa cagggaaaag ccgctggtga
     2641 taccggattt gcaggacgcg ctcgcaacct atgacgagaa caccatcacg gcagacgatc
     2701 tggtcatgat gtgttcaact cgatcgcatc gcatggagat cctcgcgaaa tccgtcagtg
     2761 agaccaattg ggcggaaaca agactgaccg agttgcagca ggagcggatc ctttccgagc
     2821 gtgccagaga gcaaggcctg gatcaggatc cggaattcgt ttctgcaagg gacaatgcac
     2881 tggcggacac cgtaatccga ctctacctca gagacaaagt ggacagaaag atcacgctga
     2941 ccgaaaagga ttttgtcaaa tactacgagt tcaacaagca gaaatacacg tctcctttgc
     3001 gcttccgatt caaccaggtc tggattgatg ttaagaagtg ggggcaagag gagggcagaa
     3061 agcgtgcgca aagtgccctc gcccgcctgc aagcgggaga aggcagcgta cccgtactct
     3121 cggaattttc cgaccgtgaa ggcgaggatc ggttcaagcc actgggtgac tacaagaagg
     3181 gggagattcc gtacacggag ttggaagaag ccgttttgag tacacccaag ggttctttca
     3241 gcgatgttgt cgagactcct ttgggttttc atatcgtcgt cgatccggaa atcactcccg
     3301 aaagcattat gagccttgaa gaggccagac cagaggtgcg ggaactgctc ttcgaagaga
     3361 agcagaaaga gaagcaggaa gccctctatg aaagtcttgc caagcgcatc aatccggtca
     3421 tccgtgtgga tttgatcgag aagaccgacg tcaatccggc tgaagttgtc ctgaaggttg
     3481 gagaaaagaa ggtgacgtcc aaggatttca aatacctgct ctcgaaatct cccaagaaaa
     3541 tgaccccacg cgaggtgctt cagggcatcg cagaacgcat gatgctctac gaggcagcga
     3601 ttcaggagaa gtacgcagaa acgaaatcct tcaagaaggc gttctcgttc atcgaggcca
     3661 agatgttgtc tgatttctac ctgaacaagg tcgcttcaga ccaaccggat ccgactgatg
     3721 aagacgtcga gaagttctac agtgactttc ctcacctgtt ctatcgagag aaagagatcg
     3781 agccctctga gattgtcatc aaggcagcca ttcgcccgga catgacacgc tatgaaaaac
     3841 tgcaggcaat gcaggatgca gagcaacgca ttcgcgatgc ccgaagacag atccttgatg
     3901 gccttccctt ctctatcgcc gccttgatgt cctcagaatc cgagacgcgc agtcagggag
     3961 ggaagatgac tcgacgcacc tggggcacag gaccgaaatt cgacaacgtg gccttctttt
     4021 tggatgaggg acagatcagc gagccggtcg agatggacga tggctatcag ttgatatggg
     4081 ttcgagcgag tcatgatcgc ttcttgcctt cattcgatga ggtgcgagag gatgctcttg
     4141 cccgactcaa acgtacccgg tacaaagaga agagagccac tcttcttgag gagacctctc
     4201 aggcaagtcc gctgaaggtg gacgctgaac tgcttggaaa gtggaaggca tggctggaac
     4261 agacggcaaa ggacgtctat ctttggtcaa agatctaaga acttctctgg agtgtgagcc
     4321 agctgacact gtgctgacag tctgaactgc actccggaat cacaggggga aaatacgggc
     4381 aatgtatgga agagctttct caagcgctgt tctgggcatt gatgcttacc tgatggaagt
     4441 ggaagtcgat ctgtctcccg gaatgcccgc attcatgatc gtcgggcttc cggacacgac
     4501 tgtcaaagag agccgcgacc gagtcaaggc agctctgacc aactcagggt tctacttccc
     4561 ggccaagaag ataacgatca acctggctcc cgccgatgta cccaaagaag gttcaggtct
     4621 ggacttgccc atggcaatag caattctggc ggcatccggg caggtggtgt cgcagaatct
     4681 cgagcactat gtttttcttg gggaacttgc cttggacggc caggtgcggc cggttcgcgg
     4741 ggtgctgccc atcgcagcgc agatgcgcaa agacaagatc gaacggctga tcgttccgca
     4801 ggcgaatgcc agtgaggcgg cggtcgtcaa aggaatcaac gttcatgggg tggaaacgct
     4861 cgtggaagtc gtagaagccc tcaacggaca gcgggaactc gcaattcatc aggtcaacgc
     4921 cgatgcggcc tttgaaaaag catcgcacta tgaagtggat atgtccgatg tcaaaggaca
     4981 ggaacacgcc aagcgggcgc tggaagttgc cgcggcgggc ggacataatt tgctgatgat
     5041 tgggccacca ggatcgggaa agaccatgct ggcgcgacgg ctgccaaccg ttctgcctaa
     5101 gatgaccttc gaagaggcgc ttgaaaccag caaaatctgg agcatcgccg gcaagatgaa
     5161 cccgggtgag gcgctgatcg cgcgaaggcc gtttcgttca ccgcatcaca ccatttccga
     5221 tgccggtctt gtcggcgggg gacgaagccc aatgccgggg gaagtcagca tgacacacaa
     5281 cggagtgctg tttctcgatg agctgccgga gttcaaccgt tc
//
LOCUS       DAOQUC010000061         5316 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_17049_length_5316_cov_3.079643, whole genome shotgun sequence.
ACCESSION   DAOQUC010000061 DAOQUC010000000
VERSION     DAOQUC010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5316
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17049_length_5316_cov_3.079643"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1357)
                     /locus_tag="PKH07_01775"
     CDS             complement(<1..1357)
                     /locus_tag="PKH07_01775"
                     /inference="COORDINATES: protein motif:HMM:NF024602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4038 domain-containing protein"
                     /protein_id="HNT33705.1"
                     /translation="MSRTITALQNQIVEWSYTSGKRYEDPFNEVDLDVIIHGLGDRMW
                     RVPAYWVGGFEWRVRFVAPEPGTYTVSTLCTDVDNSHLHGQVGTLEVAPHEADNPLMK
                     RGRLRVAASRRYLEHADGTPFFWLGDTWWMGLCKRLSWPEDFQLLTADRVAKGFTVVQ
                     IVAGLYPDMPGFDPRGANEAGFPWEEEYSRINPAYFDMADLRIEWLIRNGLVPCIFGC
                     WGYYLPIMGIEKMKRHWRYLVARWGALPVVWSLAGEGVMPYYLSKDRTGDSTKQKEGW
                     TELGKYVRELDPFHNPVAIHPTEVGRDQVEDDSILDLNMLQTGHGGYSGASRAVRFVK
                     QERDREPLMPVLVDEVNYEGILHDTHDEVQRLAYWASMLSGAFGFTYGANGIWQVNTK
                     TVPYGASPHGGNWGITPWEEAYQLPGSTQLGLAKRLLERFPWWEFEPHQDWIEPSGSD
                     SN"
     gene            complement(1381..2565)
                     /locus_tag="PKH07_01780"
     CDS             complement(1381..2565)
                     /locus_tag="PKH07_01780"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNT33706.1"
                     /translation="MNQSIIDGTRSRMTHRERFRRLMHFQTVDRGIHWEFGYLQETID
                     RWHQEGLAPEINVSEGVGSVETYFGVDPHWFVPVHFGLIPGFSGETKVLEKREKSQIV
                     QYADGMIAEVQTEGVTTIPHYIKFPIANRDDWKRYKERLDPNHEARKYDWKGFGEKAL
                     RSDQPVGINLGSYFGVPRNWIGFENLAMMCYDDRDLVEEIVETLTELYLSQLSQALKE
                     CTVDFAGGWEDICFRNGPMISPQMFREIVGPRLKRVCDLLRQHGCDVIWTDCDGDVNL
                     LIPVWLEAGLNCMFPLEVQGGSDPVPMREKYGKNILLRGGINKHQLSKGKKEIIDELK
                     RVEKLVQDGGFIPGVDHRCAQDVSYENYKYYIREKLAMLGWRHDEIAHVWPIRHMKAE
                     GW"
     gene            2769..4769
                     /locus_tag="PKH07_01785"
     CDS             2769..4769
                     /locus_tag="PKH07_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNT33707.1"
                     /translation="MGKKLAWGIIGTGSIARRFAQSLPHSETGELIAVASRTQEKADK
                     FGAEFKVQNCFGDYQALLNSKDVEAVYISTPHPMHAEWTIRAAEAGKHILCEKPFGVN
                     RAEAMAMIEAARRHDVFLMEAFMYRCHPQTARLVELIREGVIGEVRSIQSTFSFHANV
                     PPEQRLRSNDLAGGGIMDVGCYPVSLCRLIAGAAVGKDFADPVDVKGFARFDPETNVD
                     LNASGILRFPGEILAQVACGVQLTQDNSTRIYGTLGNIVVPWAWVPAVEGGKTSFFVN
                     RQGQPSEEVVTETDLWLYGIEADTCAKYLDARQASSPAMSWADTMGNMTTLDRWRESI
                     GLVYNMEKPEAMLQTVHKRPLAVKKPTRMKYGTIQGVSKQVSRLVMGVDNQMYFPSAS
                     VMFDDFFEQGGNCFDSAYVYGGGQLERILGQWIKNRGVREQVVILGKGAHTPFCDPKN
                     LTKQFLESLERLQTDYVDIYMMHRDNLDIPVGEFVEVLNEHLNAGRMRAFGGSNWTLE
                     RVRQANEYAAQKGLRGFSAVSNNFSLAEMIEAPWAGCIASSDAHSRKWFETTQIPLMS
                     WSSQARGFFTERANPGSLSDSELVRCWYSEDNFKRQARAKELAAKKGVEPIQIALAYV
                     LCQPFPTHALIGPRSLAEFWSCLGALEIQLSSDELRWLNPDA"
     gene            4846..>5316
                     /locus_tag="PKH07_01790"
     CDS             4846..>5316
                     /locus_tag="PKH07_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020249000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNT33708.1"
                     /translation="MRVKRFSVGFTLIELLVVVAIIGILAAIAIPNFLNAMARARVAR
                     AKSEMTMLASMLECYYVDQNCYPPSCGVGPYFDPSWYANPVSLRLIPLTTPVAYMSSV
                     PKGDPFPPNALPDVTAPIFDDYDTYDYMDAANRRGWGSSTCSGGIWRLASSGPDR"
BASE COUNT         1220 a         1435 c         1410 g         1251 t
ORIGIN      
        1 tattggagtc ggagccactc ggctcgatcc agtcctgatg aggctcgaac tcccaccacg
       61 gaaagcgttc gagaagccgt ttggcaagtc cgagctgcgt tgatccaggg agctgatacg
      121 cctcttccca aggtgtgatt ccccagttac cgccatgtgg tgatgcaccg tagggaaccg
      181 tcttggtgtt gacctgccaa atgccgttgg caccgtaggt gaatccaaac gcccccgaca
      241 gcatggacgc ccaataagcc agcctttgca cttcgtcatg ggtgtcgtgg agaatccctt
      301 cgtagttgac ttcatcaacc agcaccggca tcagaggctc acggtcgcgc tcctgtttga
      361 cgaaacgaac ggctctggat gcgccgctgt agcctccatg tcctgtctgc agcatattca
      421 aatccagtat tgagtcgtct tcaacctgat cgcgaccgac ctcggttgga tgaatggcca
      481 ctggattgtg aaaaggatcc agttcccgaa cgtatttgcc gagctcagtc caaccctctt
      541 tctgctttgt gctgtcgcca gttctatcct ttgacaaata gtaaggcatg accccttcac
      601 cggcgagaga ccacacaacg ggcagcgcac cccatcgggc gaccagatag cgccagtgcc
      661 ttttcatctt ctcgattccc ataatgggta agtagtaacc ccaacaaccg aaaatgcatg
      721 gaaccagacc gttgcgtata agccattcta tgcgaagatc cgccatgtcg aaatacgcag
      781 ggttgattcg agaatactct tcttcccacg ggaatccggc ttcgttcgca ccacgcggat
      841 caaatcccgg catgtcggga tacagtcctg cgacaatttg aaccaccgtg aaccctttgg
      901 caacgcggtc ggcagtcagc agttggaagt cctccggcca ggacaggcgt ttgcacagac
      961 ccatccacca ggtatcgcca agccagaaaa aaggagttcc gtctgcgtgt tcaaggtatc
     1021 gtcgcgatgc ggcgacacga aggcgcccgc gcttcatcag gggattgtcc gcctcatgag
     1081 gcgcgacttc aagagttcca acctgaccat gcagatgcga gttgtctacg tcggtacaca
     1141 aggttgagac ggtataggtg ccgggttctg gggcaacaaa tcgaacacgc cactcgaatc
     1201 ccccgaccca ataggcaggc acacgccaca ttctgtcccc aaggccgtgg attatcacat
     1261 ccaggtccac ttcgttgaat ggatcctcgt agcgtttgcc tgatgtgtaa gaccattcaa
     1321 ctatctgatt ctgcagtgct gtgatcgttc ggctcatttg atgccacctc ccggttcatt
     1381 ctaccagcct tccgctttca tgtggcgaat cggccagacg tgagcgattt catcatggcg
     1441 ccagccgagc atcgcaagtt tttcccgaat gtagtacttg tagttttcgt aggagacatc
     1501 ctgagcgcag cgatggtcta cgcccggaat gaagccacca tcttgaacga gtttctcgac
     1561 gcgcttgagt tcgtcaatga tctctttctt gcctttggac aactgatgct tgttgatgcc
     1621 gccgcgaagc agaatgttct tgccgtattt ctcacgcatg ggaaccggat cggagcctcc
     1681 ctgaacttcc agagggaaca tgcagttcaa tcccgcttcg agccagacag ggatcaggag
     1741 gtttacgtct ccatcacagt ccgtccagat cacgtcgcag ccgtgttgcc gtaatagatc
     1801 acagaccctc ttgagtcgtg gaccgacaat ttcacggaac atttgcgggc tgatcatcgg
     1861 gccatttcgg aagcaaatgt cttcccaacc gcccgcgaag tccacggtgc attctttcaa
     1921 agcctggctc aattgggaca ggtacagttc cgtcagagtt tcgacaatct cctcaaccag
     1981 atctctgtcg tcatagcaca tcatggcgag attctcaaaa ccgatccagt tgcgaggcac
     2041 gccaaagtaa ctgcccaaat tgatgcccac aggctgatcg gagcgaagag ccttttcgcc
     2101 aaaacccttc cagtcgtatt ttcgtgcctc atggttggga tcgagacgtt ctttgtagcg
     2161 tttccaatcg tctcggttgg caatgggaaa cttgatgtag tggggaatgg tggtgacacc
     2221 ttcagtctgt acctcagcga tcatgccgtc cgcatattgc acgatctgag acttctcgcg
     2281 cttttcgagc acttttgttt cgccgctgaa gcctggaatc agtccgaaat gcaccggaac
     2341 aaaccagtgc ggatccactc caaaataggt ctcgacagag ccgacccctt cggaaacatt
     2401 gatctcagga gccagtcctt cttgatgcca tcggtcaatg gtctcctgca agtagccgaa
     2461 ttcccaatga atccctctgt ccaccgtctg aaaatgcatc aggcggcgaa atcgctctct
     2521 gtgtgtcatc cgagaccttg tgccatcaat aatgctttgg ttcatgctct acccttcccg
     2581 tcagtcgtcg gtcaatgccc attgatacat ggactcaatt tgggaaactc taatgaaatc
     2641 ggattctttg tcaatcggaa gtgccccctt cagcaggagg ctctcatctc tgttgacact
     2701 ctctgcgttc tatgtcaaaa gatcctcgtt ctcattgaag tcgagctaca catatcagga
     2761 ggctttccat gggcaagaaa ctggcatggg gcattatcgg aaccggctcg atcgcacgac
     2821 gctttgcgca atcactcccg cactcagaga caggcgaact gatcgctgtc gcgagtcgga
     2881 cacaggaaaa ggcggacaaa tttggtgccg agttcaaagt ccaaaattgt tttggagact
     2941 atcaggcact cttaaatagc aaggatgtgg aagcagtcta catctcgacc ccccatccca
     3001 tgcatgccga atggacgatc agagcggcgg aggcgggcaa acacattctt tgtgagaagc
     3061 ccttcggtgt caatcgagca gaagcgatgg cgatgatcga agcagcgcgt cgccatgatg
     3121 tgttcctgat ggaagccttc atgtatcggt gccatccaca gacggcgcgg ttggtcgaac
     3181 tgattcgaga aggggttatt ggtgaagtcc gctcgattca gtctacattc agtttccatg
     3241 caaacgttcc gcccgagcaa cgcttgagaa gcaacgatct ggccggtggt ggcatcatgg
     3301 atgtgggttg ctacccggtt tcactgtgcc gtctgattgc cggtgcggcg gtaggaaagg
     3361 actttgccga tccggttgac gtgaaaggct ttgcccgatt tgatccagag acaaacgtgg
     3421 acttgaatgc ttcagggatt ctgagatttc caggggagat tcttgctcaa gtggcgtgtg
     3481 gtgttcaact gacgcaggac aattcaactc gaatctacgg aacactcgga aacatcgtgg
     3541 ttccctgggc atgggttcca gccgttgagg gtggcaagac ctctttcttt gtcaaccgtc
     3601 agggacagcc ttcagaagag gtggtcaccg aaacagacct gtggctctac ggcatcgaag
     3661 ccgacacctg tgcgaagtat ctggatgccc gccaggcatc ttcacccgcc atgagctggg
     3721 cagatacgat gggcaatatg accacgctgg acagatggcg tgagagcatt gggctggttt
     3781 acaacatgga aaagcccgaa gcaatgcttc agaccgttca caaacgtcca cttgccgtga
     3841 agaaaccaac acgtatgaaa tacggaacga tacaaggcgt cagcaagcag gtctctcggc
     3901 tggtgatggg cgtggacaac cagatgtact tcccgagtgc ctctgtcatg ttcgacgatt
     3961 tctttgagca ggggggcaac tgtttcgaca gtgcctatgt ctacgggggc ggtcagctgg
     4021 agcggattct cggtcagtgg atcaagaacc gaggtgttcg cgaacaagtc gttattctgg
     4081 gaaaaggagc gcatacgcca ttctgtgatc ccaagaatct gaccaaacaa ttcctcgaaa
     4141 gcctggaaag gttacagacg gactatgtgg acatctacat gatgcaccgc gacaatctgg
     4201 atattccagt cggggagttt gtcgaggtgc tcaacgagca tctcaacgcg ggtcgtatgc
     4261 gtgccttcgg tggatcgaac tggacactcg aacgagttag gcaggccaat gagtatgccg
     4321 cccagaaggg actgcggggt ttctccgctg tcagcaacaa tttcagcctt gctgagatga
     4381 ttgaggctcc ttgggcggga tgtatcgcca gctcggatgc acactcccga aagtggtttg
     4441 agacaaccca gattcctttg atgtcatggt caagccaggc acgcggattc ttcactgaaa
     4501 gagccaatcc aggcagcctg tccgactccg aactggttcg atgttggtac agtgaagaca
     4561 acttcaaacg tcaggctcgt gccaaagaac tcgccgccaa gaaaggcgtt gagccgattc
     4621 agatcgcttt ggcatacgtg ttgtgccaac cgttcccgac tcacgcactc attgggcctc
     4681 gctcgctcgc agaattctgg tcatgtctgg gagcgctgga gatacaactc tcttctgacg
     4741 agttgcggtg gttgaatcct gacgcctgaa gcgtcttgcc gctatgatga cgcctgtcag
     4801 gcgcagagtg gagcagttgc tgactgcaac ggccgaggaa cactcatgag agtcaagagg
     4861 ttcagcgttg gtttcactct catcgaactg cttgttgttg ttgcgattat cgggattctc
     4921 gctgcgatag ccattccaaa ctttctgaac gcgatggcta gagcaagagt ggcaagagcc
     4981 aaatcggaaa tgacgatgtt ggcgtcaatg cttgagtgct actatgtgga tcagaactgc
     5041 tatccaccct catgtggggt agggccctat ttcgaccctt cgtggtatgc caacccagta
     5101 agcctccggc tgatcccatt gacaacaccc gtggcgtata tgagttctgt acccaagggt
     5161 gatccgtttc cgcccaatgc gctccccgat gttactgcac cgatttttga cgactatgat
     5221 acctatgatt acatggatgc agccaaccgc cgtggatggg gcagcagcac atgttctgga
     5281 ggaatctggc gacttgccag ttctgggcct gacagg
//
LOCUS       DAOQUC010000062         5313 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_17065_length_5313_cov_3.586154, whole genome shotgun sequence.
ACCESSION   DAOQUC010000062 DAOQUC010000000
VERSION     DAOQUC010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5313
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17065_length_5313_cov_3.586154"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(190..399)
                     /locus_tag="PKH07_01795"
     CDS             complement(190..399)
                     /locus_tag="PKH07_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FlbD family protein"
                     /protein_id="HNT33709.1"
                     /translation="MIQLTHLRGAEFLLNAELIEFIENTPDTVVTMVSGKKYLVQESL
                     QEIQQRVVDYRRRCLEPRTRNEDPV"
     gene            complement(520..2673)
                     /locus_tag="PKH07_01800"
     CDS             complement(520..2673)
                     /locus_tag="PKH07_01800"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HNT33710.1"
                     /translation="MLRSLFAGVSGLRSHQVLMDVLGSNIANVNTVGYRSGRMTFQDQ
                     MSQTLSGAQGATTTRGGVNPAQIGLGSQVGAIDTLFSQGNFQNTERLLDLGIEGKGFF
                     QLVDQAGGIYFTRAGNFSLDSDGYIVNPSNGLKLFGLLADRTGSINETQPAESIRIDY
                     SMQADAQVTRNVTLGGNLDSRIQPTTASSRNQLLGLFDEYGRSLNLRIGDVIEFSGGF
                     VDKNGNGILDGGDVNLTDLTITTVGSDTTLADLAKAMETALRAATSSTSIAVSVNPDG
                     SIKVSTDSLNPIVGMTVDVDGAPNSILRGIFQDADGDQDIDVVASGSGNTRTMRQADV
                     TNSINVYDSQGNERTLTIAYARATNHVPISSDSLLTGLYTQSGVAMNVLVGDVIEFPA
                     GTIGDFDLSTSGFTFTVTESSTIADLLSAIETALESVDTDSGTAGDQFASVSVSMNPD
                     GTLSITSDTAITGLQIDTQTTSNPGLMGLFEEFGTTALNGINVSAGSTVTTNAGMVSS
                     LNTWNWRAIVPHPAQVFPTNDAGTVIFNSDGTFQSYGSSGLAAPVIKFDPDGTGAEND
                     GVDELSITMDMGRLTQNASASTAAIRTQDGRGIGTLDSITIDPSGIITGVFTNGASMN
                     LAKIQLAYIPNEGGLRRVGDTMFQESSNSGIPVLGNAGEGGRGTISSGTLELSNVDLA
                     QQFTNLIVAQRGFQANSRIITTSDEILTEVVNLKR"
     gene            complement(2766..3161)
                     /locus_tag="PKH07_01805"
     CDS             complement(2766..3161)
                     /locus_tag="PKH07_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019910410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02530 family flagellar biosynthesis protein"
                     /protein_id="HNT33711.1"
                     /translation="MISKINGHLFTPQTAKVSPESVPQAIRQPESPALSFAEVLTQKQ
                     ASLSSLKMSAHAQQRLQSRNIQLSAMDMDRISDAVDKASAKGARETLVVMRDMALVVS
                     AQNRTIITAMDGANMRDNVFTQIDSAVIL"
     gene            complement(3197..3940)
                     /locus_tag="PKH07_01810"
     CDS             complement(3197..3940)
                     /locus_tag="PKH07_01810"
                     /inference="COORDINATES: protein motif:HMM:NF025229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HNT33712.1"
                     /translation="MAVGTIQSTTSGSDAQIFSQANILGKDDFLKLLVAQLANQDPLE
                     PMKDQEFVAQLAQFSSLEQLKNLDKSLASLLNLQESSINALSVGLIGKTARVYASTVD
                     TSLMEGPVTFELYPPSGTRVHLKIFDANGYVVREQMLESEAGQVVFEWDGTDAEGNVL
                     ADGQYTVKAYSSDTGTAEPIQYPIFVVGTVRSVDFSSGTALVNVNGVVVDLSRVIEVR
                     NGPESTMVETGGAESRLALNPTSWMMRRK"
     gene            complement(3971..>5313)
                     /locus_tag="PKH07_01815"
     CDS             complement(3971..>5313)
                     /locus_tag="PKH07_01815"
                     /inference="COORDINATES: protein motif:HMM:NF014207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HNT33713.1"
                     /translation="SQRTIDQAVIRFPLSENTQEPYRIDELLRMKTETSSSSPLEGLL
                     QRSSVPLPRNPGTSSLPLQGADLKSPEMKNVPQIGMKLSFSEVPSQPAKEEIQPPMIK
                     QQTADAQVLSKSESKVVEDSVKPGDAMVFKNILGTHRETTVAKGVFAAAHADSSLSIS
                     RSVQDTQIKSSWNPFKMFSARAERSETRQSVPSAPRDEALAERRAQNAPDRRPVLRAE
                     RAPVDRSVTEDRRSESQRRGDMGEMRPKTESALRNPSEPHEVRNRPESSSLLTPRSDA
                     AVSRGTVATPQIESSPRVFSQPLVEQVADQVGPAMRGGISEMTIRLEPEDLGPVQVKV
                     TLEEGLVVARLQSGNGETRGILERNLQQLAQALREQGIPLAEVRVNATTATSDRTNAE
                     RERPSNYGEQRQGGNHGGQGGSDGRQQQRQERRWWWMSYNQDSDPTSRKYYQGV"
BASE COUNT         1178 a         1483 c         1367 g         1285 t
ORIGIN      
        1 ggttgtaaaa gagaagcagg cttcctccga gcatgattgc ccagaggaca acgataacgc
       61 acaggatgta cccgatcaga gttgatttat ccatggaagt ctaatgctca cctcggatgc
      121 cctgaacttg agagtgctca gagtatctct gtcccgacag atgttttata cccgaccctg
      181 ttcgaagagt caaaccgggt cttcgtttcg agtccttggc tccagacagc ggcgtcggta
      241 atcaacaaca cgctgctgaa tctcctgcag tgactcctga accaggtatt tcttccctga
      301 aaccatggtc acaacagtgt ccggagtgtt ctcgatgaac tctatcaact ccgcattcaa
      361 gagaaactct gcgcctcgaa gatgcgtcag ttggatcatg gtctgggtgc ctccccgttc
      421 tcaggccggc gcaaaacccc gacgggtttc agcgttcatt cgacctttgt ttcaggagtg
      481 agggagggga ctttgcccct ccctcacaga atgatcgaat taccgcttga gattcacaac
      541 ttccgtcaga atctcatcac tggtcgtgat gatacgagag ttggcctgga atcccctctg
      601 cgccacaatc agatttgtga actgttgggc gagatccacg ttggacaatt ccaaggtgcc
      661 agaggagatt gttcctcgac caccctcacc cgcattgccg agcaccggaa tacccgagtt
      721 gctggactcc tgaaacatcg tgtctccgac gcgacgcagg ccaccttcat tcggaatgta
      781 ggccagctga atcttggcaa gattcattga agcgccattg gtgaagacac cggtgatgat
      841 accactcgga tcaatggtaa tcgaatccag cgtgccaatg cctcgaccgt cctgggtgcg
      901 aatcgcagcc gtcgaagccg atgcgttctg ggtcagacgg cccatgtcca tggtgatgga
      961 cagttcatcc acgccatcat tctcagcacc ggtgccatcc ggatcaaact tgatcacagg
     1021 ggctgcaaga cccgacgaac catagctctg gaacgtgccg tcagagttga agatgactgt
     1081 tcctgcatca ttggtgggga atacctgagc tggatgagga acgatggctc gccagttcca
     1141 agtattcagg ctggaaacca tgccggcatt ggtagtcact gtggaaccag cgctcacgtt
     1201 gattccgttc aatgcggtgg tgccaaattc ctcaaacagt cccatcaagc cagggttgct
     1261 ggtggtctgt gtatcaatct gaagacccgt gatagccgta tccgaagtga ttgagagtgt
     1321 gccatcaggg ttcatggaga ccgaaacact ggcaaactga tcccctgccg taccagagtc
     1381 tgtgtctaca ctctcgagtg cggtttcaat ggcggacagc agatcggcaa tggtagagga
     1441 ctccgtcacc gtgaacgtga atccactggt cgaaaggtcg aaatcaccga tggtgcccgc
     1501 cgggaactcg atgacatcac cgacaagcac gttcattgca actcccgact gggtgtaaag
     1561 acccgtgagg agagagtctg aggagatcgg aacatggttc gtcgcgcgag cataggcaat
     1621 cgtcagtgtt ctctcgttgc cttgagaatc atagacgttg atggaattgg tcacatccgc
     1681 ctgcctcata gtccttgtgt tccctgaacc ggaagccacg acgtcaatgt cctgatcgcc
     1741 gtccgcatcc tggaagattc ctcggagaat tgagttgggc gctccatcca cgtcaaccgt
     1801 cataccgacg atcggattga gactatccgt agaaaccttg attgatccat ctggattcac
     1861 cgaaaccgca atagaagtgg aactggtcgc tgcacgcagg gctgtttcca tggccttggc
     1921 aagatccgca agggtcgtat cggatccgac ggtggtgatc gtgagatcgg taagattcac
     1981 gtcacctcca tcaagaatgc cattgccgtt cttgtcaaca aaaccaccgc tgaattcaat
     2041 tacatcaccg atacgcagat tcaacgagcg tccgtattca tcgaacagac ccagaagctg
     2101 atttcggctg ctggcagtcg tcggctggat tcgggaatcc agattgccac cgagggtgac
     2161 attgcgggtc acctgtgcat ccgcctgcat gctgtagtca attcgaatgg actcggccgg
     2221 ctgggtctca ttgatcgaac cggtgcggtc tgccaggagt ccaaagagtt tgagaccatt
     2281 ggacggattc acaatgtacc cgtcggagtc caaactgaag ttaccggctc gggtgaaata
     2341 gataccccca gcctgatcca ccagctggaa gaatcccttg ccttcgatac cgagatccaa
     2401 cagccgttct gtgttctgga agttgccctg actgaacagc gtatctatgg ctccgacctg
     2461 tgagccaaga ccaatctggg cagggttcac cccaccacga gttgtcgttg ctccctgggc
     2521 accggaaagc gtttggctca tctgatcctg aaaagtcatc ctgcccgaac gataaccgac
     2581 cgtgttgaca ttggcgatat tgctgccaag tacatccatc aatacctggt gtgaacgcaa
     2641 accggacaca ccagcgaaga gtgaccttaa catttgaaaa cctcctgaat tgccgcttcg
     2701 cgtcagtcgt tcggcgaagc gttgggcttc ctcatagagg tccagccctt gtccttgttt
     2761 atttgctaaa gaatcaccgc gctatcaatc tgcgtgaaga cattgtccct catgtttgcg
     2821 ccgtccatgg ctgtgatgat ggttcgattc tgagcactca ccaccagtgc catgtcacgc
     2881 ataacgacga gagtctcgcg agcaccttta gctgacgcct tgtccactgc atccgaaatc
     2941 cggtccatgt ccatggcgct cagctgaatg ttgcgacttt gcagccgctg ttgcgcatga
     3001 gcgctcatct tcaggctcga aagcgatgcc tgcttctggg tcaacacctc ggcaaaactc
     3061 agagctgggg actccggttg ccgaatcgcc tgcggaacgg actccggaga gacctttgcc
     3121 gtctgcggtg tgaagaggtg cccgtttatc tttgatatca cctgtcatcc ctcctttcac
     3181 tataaacgtt ctgggattac ttccgcctca tcatccagga tgtcggattc aaagcgaggc
     3241 gagattccgc cccaccggtc tccaccatcg tgctctctgg tccgttgcga acttcgatca
     3301 cccgcgacaa gtctacaacc acgccgttga cattcaccaa agctgtcccc gaggagaaat
     3361 ccacactccg aacggttcca acgacaaaga tggggtattg gataggttct gctgtacccg
     3421 tatcagacga atacgccttg acggtatact ggccatcggc cagaacattg ccttcggcgt
     3481 ctgttccatc ccactcgaaa acaacctgtc ctgcttcact ctcaagcatc tgctccctga
     3541 cgacataacc attggcatcg aatatcttca ggtgaaccct tgtcccactg gggggataca
     3601 gttcaaatgt gacgggacct tccataagcg aagtgtccac ggtgctcgca tagacgcgcg
     3661 ccgttttgcc gatgagtccc acggacaagg cgttgatgct tgactcctga aggttcaaca
     3721 gagaggcaag agacttgtct agattcttca actgctccag agagctgaat tgtgccaact
     3781 gtgcaacaaa ttcctgatct ttcatcggtt ccaaaggatc ctggtttgcc aactgtgcca
     3841 ccagaagttt gaggaaatca tctttgccaa gaatgttggc ttgggagaag atttgagcgt
     3901 cggatccaga agtcgtactc tgtatcgttc ctactgccat aaaaactcac ctccgttcag
     3961 gtctggttgt tcacacgccc tgatagtatt ttcggctggt tgggtcggaa tcttgattgt
     4021 aactcatcca ccaccagcgt ctttcttgac gttgttgctg tctgccgtct gatccgccct
     4081 gtccaccgtg attgccccct tgtcgctgtt ctccatagtt tgaaggacgc tcgcgttcgg
     4141 cgttcgttcg atccgaggtc gccgtcgtgg cattgacacg cacttcggcc aatggaattc
     4201 cctgctcccg aagggcttgc gccaattgct gaagattgcg ttcgagaatg ccgcgtgtct
     4261 ctccattgcc actttgaagc ctggcaacca ccagaccttc ctcaagagtg accttcacct
     4321 gaaccggccc aagatcttct ggctcaaggc gaatggtcat ctcgctgatt ccacctctca
     4381 tcgcggggcc cacctgatcc gcaacctgct ccaccagagg ctgagagaat acgcgtggcg
     4441 aagactcgat ctgaggcgta gccacagtcc ctcgactgac ggcagcgtca gatctgggcg
     4501 tgagcaagga agaagactcc ggacgatttc gaacctcatg aggctcagag ggatttctga
     4561 gtgccgattc ggttttcgga cgcatctcgc ccatgtcgcc tcgacgctgt gactcagagc
     4621 gcctgtcttc cgtgacggaa cggtcaactg gagcacgctc agcacgtagt actggtcttc
     4681 ggtctggcgc attctgcgcg cgccgctctg ccaaggcctc atctcttggt gctgaaggca
     4741 cagattgtct tgtctcactt cgttcagcgc gcgcactgaa catcttgaag ggattccacg
     4801 acgacttgat ctgcgtgtct tggacggatc ttgagatcga gagagacgaa tcagcatgtg
     4861 ctgctgcgaa gacacctttg gcgactgtcg tttccctgtg tgttccgagg atgttcttga
     4921 acaccattgc gtcaccgggc ttgaccgagt cttctaccac tttggactca ctctttgaca
     4981 gtacttgggc atctgcagtc tgttgtttga tcattggcgg ttgaatctct tctttcgcgg
     5041 gctgactggg aacctcagaa aacgacaatt tcataccgat ctgtggcaca ttcttcatct
     5101 cagggctctt gagatcggcg ccttgcaggg ggagagagga tgttccgggg tttctgggga
     5161 gagggacaga tgacctctgc agcaaaccct ccaaaggact gctcgaggaa gtctccgtct
     5221 tcatccgaag taactcatcg atcctatacg gctcctgagt attctcgctc aaagggaatc
     5281 ttatcactgc ctgatctatg gttctttgag att
//
LOCUS       DAOQUC010000063         5299 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_17137_length_5299_cov_3.210717, whole genome shotgun sequence.
ACCESSION   DAOQUC010000063 DAOQUC010000000
VERSION     DAOQUC010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5299
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17137_length_5299_cov_3.210717"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..495
                     /locus_tag="PKH07_01820"
     CDS             <1..495
                     /locus_tag="PKH07_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010938692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HNT33714.1"
                     /translation="VLNKARAMELQAIHQYMNQHYNLDDMDYGEMAAQVKLIAIDEMR
                     HAEMFAERIKELGGEPTTEAAGKVLKGQAVEKVFAFDADLEDNTMAIYNKFVLTCREN
                     GDSTSQKILEAIIDEEQAHFNYFDNVGEHIERLGAAYLAQKAGTSSATGLNTQGFVAR
                     KAAQ"
     gene            complement(505..2340)
                     /locus_tag="PKH07_01825"
     CDS             complement(505..2340)
                     /locus_tag="PKH07_01825"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33715.1"
                     /translation="MDEKSRSEASMGRSGNLTTEKPCYQPLDVVRVTIHGRDAGDTQC
                     TLRVCDPQQKTYLEKQVALKNNRGEATFLAGGSLGVHYIYLLWPGEKRHSRYLNFWLD
                     CETTVESGDEDIDFIYPFTRDRMRLGRREYDTPQGKFVGYMSADTWHFDGIWLRDWIY
                     GLPGYKYWERDMACGIDRFLEQQAENGMIPDGIERSGKTWRVGLESDVEYIMVMGAWG
                     TWKATGDDAWLVSILPKLEKALEYVRSDPKHWDESHRLIKRQHSCDTWDFDIDSAGDS
                     GRQRHVVATCDQSGYVLAFQALSQMYGRVGCCPDAHRWEREAEEYRKRANQMLWDGVK
                     FQHHVHLDAIDHNGFDESQQLAMGNTWAMTRGLATRVQAQSIIDEYKRRHEQTGDAYP
                     WWSLQPGYPDELGYFGSAHRKQGGYANGGLMPWVGGELCRAAFRFGREKYGVELMRQY
                     VDHLRRTGGAHVWYWPDGEPGFRTTNEVPYAGWGMAQWVEALFEGLAGVEDRSCLMEQ
                     VRLNPQWFACGVQSVLVRTRYAPSKGYFAYRMTTKKKPRSVVIDYSGSGRSVAFQIPV
                     PDGWTPKTVTVNGESVEFSLSPDSPNRVCFSVEMQSIGQATVLCD"
     gene            complement(2504..3211)
                     /locus_tag="PKH07_01830"
     CDS             complement(2504..3211)
                     /locus_tag="PKH07_01830"
                     /inference="COORDINATES: protein motif:HMM:NF021492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNT33716.1"
                     /translation="MARRTRIQVKRKKLSDKEVHELAKQDPLLKMMNQSLDFFKQRGQ
                     VIIAVIIVAAVGFFAIRGYLSYRANQEMDAGKILATAEEALYQNELDKAASVLDSLLK
                     DHSGTRTSKQAEMLKASVLLAKGNTEEAIQLYRDLLSRNRGEIARELKFSLANALQSK
                     GDAEEALRLYEELEKDGAFVRQYELLYRKGQVLESLNQYDGARESYSKIPSDSESVYY
                     GLARQRLEWIRAMANAA"
     gene            complement(3241..3936)
                     /locus_tag="PKH07_01835"
     CDS             complement(3241..3936)
                     /locus_tag="PKH07_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006919048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase
                     domain-containing protein"
                     /protein_id="HNT33717.1"
                     /translation="MAKKNSVLPIIDLHTHSLFSDGLLMPAELVRHAQTAGYHAIVLT
                     DHVEESNLEMAMDLTVRACETLRDRIGIVLVPGAEISYVPPRRIPAVAERARELGAAV
                     LVVHGETLVEPVPPGTNEAALDADIDLLAHPGLISRKLASRAAERGISLEITTRRGHC
                     YSNGHVCRVAREAGAMLTVNNDAHMPGDSLKPEMRRCVALGSGMLDAEYEKACRNAVK
                     ILGRRGFRSIPIR"
     gene            complement(4135..4845)
                     /locus_tag="PKH07_01840"
     CDS             complement(4135..4845)
                     /locus_tag="PKH07_01840"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HNT33718.1"
                     /translation="MEPKACVLIATYNERQNIDQMISALLSLPNNFEVLIVDDSSPDG
                     TGERVGELAKCEPRLHLLSRKNKEGYGPAMIAGLRYALQLGVERIVTMDADFSHDPKD
                     VPKVCAALEKADIVLGSRYRGGVRVLNWAPRRLLLSMGANRYVRSVLGLSVADCTSGF
                     RGYRRGVFESDILERCRFRGYAFLVELLYRCLKRGVHVAEVDIIYTERREGHSKMSKR
                     VIFEAVFAPWILRFFTRR"
     gene            complement(4864..>5299)
                     /locus_tag="PKH07_01845"
     CDS             complement(4864..>5299)
                     /locus_tag="PKH07_01845"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012031815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HNT33719.1"
                     /translation="VCDGALYYVLGSLVTDIAPGYDHITSAIGGTLAAIAGADYLCYV
                     TPAEHLRLPTLEDVRNGVIASRIAAHAADIVKGIAGASEWDDQMSKARKSFKWEDMFA
                     LAIDPETARAMRKESGLSQDEMCTMCGDYCSMRQGTQTARKN"
BASE COUNT         1225 a         1583 c         1325 g         1166 t
ORIGIN      
        1 gtcctgaaca aggcacgagc aatggaattg caggcaatac atcaatacat gaatcagcat
       61 tacaaccttg atgacatgga ctacggcgag atggctgcgc aggtgaaact catcgccatt
      121 gacgagatgc gccatgcgga gatgttcgcc gaacgaatca aggaactcgg tggcgaaccg
      181 actacggagg cggcgggtaa ggtgctgaag ggtcaggccg tcgagaaggt gttcgccttt
      241 gacgctgatc tggaagacaa taccatggcg atttacaaca aattcgtgtt gacgtgtcgc
      301 gagaacggtg acagcaccag ccagaagatt ctggaagcca tcattgatga agaacaggct
      361 catttcaact actttgacaa tgtcggcgaa cacattgaga gactcggcgc ggcctacctc
      421 gcacagaagg cgggcacctc gtctgcaacc ggcctgaaca ctcagggatt tgtcgctcgc
      481 aaggcagcac agtaagcagg ttgatcagtc acagagaacg gtcgcctgtc cgatgctctg
      541 catctcaaca gagaagcaca ctcgattcgg tgaatcagga gaaagactga actcgacaga
      601 ctcaccgttg accgtcacag tcttgggcgt ccagccatct ggaacgggga tctgaaacgc
      661 aaccgaacga cctgagccgg agtagtcaat aaccacggag cggggctttt tctttgtagt
      721 catccgatag gcgaagtatc cctttgaagg ggcgtatcga gttcggacaa gaaccgattg
      781 gacgccacag gcaaaccatt ggggattcag tcgaacctgt tccatgagac aggaacggtc
      841 ttcaacaccc gccaggcctt cgaacaacgc ttcaacccac tgtgccattc cccaaccagc
      901 gtaagggact tcgttggtcg tccgaaaacc aggctcgcca tcaggccaat accacacatg
      961 agcgccaccc gttcgtcgaa gatgatccac gtactgcctc atcaactcga cgccgtactt
     1021 ctctcggcca aatcgaaaag ccgccctgca caattcccca cccacccaag gcatcaggcc
     1081 accattggcg tagcccccct gttttcgatg ggcggatccg aagtacccca gttcatctgg
     1141 ataccccggc tgaagactcc accacggata ggcatctccc gtctgctcgt gtctgcgttt
     1201 gtactcatca atgattgact gtgcctgcac gcgagttgcc aacccacgag tcatcgccca
     1261 ggtgtttccc atcgccagct gttgcgactc atcaaatcca ttatggtcaa tggcatccag
     1321 atgcacgtga tgctgaaact taaccccgtc ccacaacatc tgattggctc gcttgcggta
     1381 ctcttctgct tcccgctccc agcgatgggc atccggacaa caaccaactc gaccgtacat
     1441 ctgactcaag gcctgaaagg caagtacata gccactctga tcgcaggtgg cgacaacatg
     1501 tctctgcctt cctgaatcac cggcggagtc aatgtcaaaa tcccatgtat cgcaactgtg
     1561 ttgcctcttg attaaccgat ggctttcgtc ccagtgcttg gggtcactgc gaacgtattc
     1621 gagcgccttc tcaagtttgg gtagaattga gacaagccag gcatcgtcgc ccgtggcttt
     1681 ccaggttccc caagccccca tcaccatgat gtactcaaca tccgactcaa gcccaacacg
     1741 ccaagtcttg ccactgcgtt cgatgccatc aggaatcatc ccgttctcgg cctgttgctc
     1801 cagaaaacgg tctatgccac aagccatgtc ccgctcccaa tacttgtaac ccggcaggcc
     1861 atagatccag tctcgcaacc agatgccgtc aaaatgccag gtatcggcag acatatagcc
     1921 gacgaacttt ccctgtggcg tgtcatactc gcggcgtcca aggcgcatcc gatcgcgagt
     1981 gaacggatag atgaagtcaa tatcctcatc acccgattcc accgtcgtct cgcagtcgag
     2041 ccagaaattc agataacgac tatgcctctt ctcgccaggc cagaggagat agatgtaatg
     2101 aacacccagg gaaccacctg ccagaaacgt cgcctcgcct ctgttgttct tgagcgcaac
     2161 ctgcttttcg agataggtct tctgttgtgg atcgcacacg cgaagagtgc attgggtgtc
     2221 accggcatct cgtccatgaa ttgtcacgcg aacgacgtcc aacggctgat agcagggctt
     2281 ttccgtcgtg agatttccag agcgacccat actcgcctcg gatcgagatt tctcatccat
     2341 cgtgatgctc ctgatgtgct gatttggtct ggctcaaggt cttctgttga accgtccact
     2401 ctctggaaac gagaggagtc agattctccc ctctgaaagg gaaaatccga gaggtctttt
     2461 cagtgcagcg gacgcttggc ggctcgcggt cttgacaggg gtgttacgcg gcgtttgcca
     2521 tggcgcgaat ccactccagg cgttgacgcg ccaagccgta gtaaacggac tcgctgtcgc
     2581 tcggaatctt gctgtaactt tcacgggcac cgtcatactg gttcaaagac tccagcacct
     2641 gccccttgcg gtagagaagt tcgtactggc ggacgaacgc accatctttc tcaagttctt
     2701 catagagacg caaggcttcc tcggcgtcgc ctttgctctg aagtgcattg gcaagcgaga
     2761 acttgagttc tcgcgcaatt tccccacggt tacggctcaa caagtcccga tacaactgga
     2821 tggcttcttc ggtgttgccc ttggcaagca gaacgctggc tttcagcatc tcggcctgtt
     2881 tggatgttcg cgttccggaa tgatccttga gcagactatc caacacggaa gccgctttgt
     2941 ccaactcgtt ctggtagaga gcctcttccg cggtcgcgag aattttgccg gcatccatct
     3001 cctgattggc gcgatagctc aagtatccgc gaatggcaaa gaaacccacc gcagccacga
     3061 tgatcaccgc aatgatgacc tgtccccttt gtttgaagaa gtccagggac tgattcatca
     3121 tcttgagcag cggatcttgc tttgccagct cgtgaacttc cttgtcactg agtttcttgc
     3181 gttttacctg aatgcgcgtt cttcttgcca tgggtctttc ctcacttcaa aggatatgta
     3241 ttaccgaatc ggaatactcc gaaaacctcg tcgtccaagt atcttgacag catttctgca
     3301 tgccttctcg tactccgcat cgagcatgcc gctgcccaat gcgacacagc gcctcatctc
     3361 cggcttgagt gaatcgcctg gcatgtgagc atcgttgttg actgtgagca tcgctcccgc
     3421 ttcccgagcg acgcggcaga cgtggccatt gctgtagcag tgacctcgac gcgtagtgat
     3481 ctccagcgaa atccctcgct ccgctgcacg actcgccagt ttcctggaga tcaaccctgg
     3541 atgcgccaat agatctatgt ccgcatcaag agcagcttcg ttggttccag gaggcacagg
     3601 ttccaccagc gtttcgccat gtactacaag aactgccgca ccaagttccc gtgcccgttc
     3661 agccacagcg ggaatgcgcc ggggtggaac atagctaatc tcagcaccgg gaacgagaac
     3721 aatgccgatt cggtcgcgga gtgtctcaca ggcgcgaacc gtcaaatcca ttgccatctc
     3781 aagattagac tcttcaacgt gatcggtcag cacgatggca tgatagccag ccgtctgcgc
     3841 atgacgcacc aactccgccg gcatcaggag cccgtcgctg aagagtgaat gtgtgtgcag
     3901 gtcaatgatc ggcaacacac tgtttttctt cgccatggaa tcttcccttt cctggaatca
     3961 tccaaagtta cacacagcag agacacccaa gtctccgtct taaacgactg ccttcgagga
     4021 agcccgattg ccaacaccca atggctgtcg cccgcctcga acttccgcgg aagttgataa
     4081 cacaaaacca catcccgtgc aacgcggaaa cgccgagccc aacacgtgag gcgatcatct
     4141 tcgcgtgaag aacctcagaa tccagggcgc aaaaactgcc tcaaaaatca ccctcttgga
     4201 cattttcgag tgcccttccc gtctctccgt gtagatgata tccacttcag cgacgtggac
     4261 gcctcgcttc agacagcgat acagcagttc aacgagaaac gcgtaaccgc ggaatcggca
     4321 tcgttccaga atgtcgcttt caaagactcc ccgtcggtat ccccgaaagc cgctggtgca
     4381 gtcggcgaca ctcaggccaa gcaccgaacg aacgtatcgg ttggctccca tggagagaag
     4441 aagccttcgc ggcgcccagt tcaaaacgcg aaccccaccg cgatagcggg aacctagaac
     4501 aatgtcggct ttctccaacg cagcgcacac ttttggaaca tccttgggat catgggagaa
     4561 gtccgcatcc atcgtgacga tacgctccac accaagttgg agcgcatatc tgagacccgc
     4621 gatcatcgcc ggcccatagc cctctttgtt ctttcgtgag agaaggtgga gccgcggttc
     4681 acacttggca agctcgccaa ctctctctcc agtaccatct ggggaactat cgtctacgat
     4741 gaggacttca aaattgttgg ggagagaaag cagcgcagat atcatctggt ctatgttctg
     4801 acgctcattg tacgtagcga tgagaacgca tgccttgggt tccaccgact ctccccccat
     4861 agtctagttc ttcctggcgg tctgcgtccc ctgtctcatg gagcaatagt caccgcacat
     4921 ggtgcacatc tcgtcctggc tcagaccgct ctctttgcgc atcgcacggg cagtctccgg
     4981 atctatggcg agcgcaaaca tatcttccca cttaaacgac tttctggctt tggacatctg
     5041 atcatcccat tcggatgctc cagcaatgcc cttaacgatg tccgccgcat gcgctgcgat
     5101 gcgcgaagcg atgactccgt tacgcacatc ctcaagtgtc gggagccgga gatgctccgc
     5161 cggtgtgaca taacacaagt agtcggctcc tgcgatggcc gccagggtgc cgccgatggc
     5221 ggaggtgatg tgatcgtacc ccggtgcaat gtccgtcacc agagaaccga gcacgtaata
     5281 gagtgcacca tcgcagacc
//
LOCUS       DAOQUC010000064         5297 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_17142_length_5297_cov_3.392407, whole genome shotgun sequence.
ACCESSION   DAOQUC010000064 DAOQUC010000000
VERSION     DAOQUC010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5297)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5297)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5297
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17142_length_5297_cov_3.392407"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            112..375
                     /locus_tag="PKH07_01850"
     CDS             112..375
                     /locus_tag="PKH07_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015858324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015075 - monoatomic ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter complex subunit
                     F"
                     /protein_id="HNT33720.1"
                     /translation="MSFLTFITFTLLLISLGSLYRVIFGPTPHDRLVGLNLLVALVTG
                     VMVLLAVLYKRDIYLDVALVYTMLGFISVIAVTKHLKGKGLSE"
     gene            372..704
                     /gene="mnhG"
                     /locus_tag="PKH07_01855"
                     /pseudo
     CDS             372..704
                     /gene="mnhG"
                     /locus_tag="PKH07_01855"
                     /inference="COORDINATES: protein motif:HMM:NF015302.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H(+) antiporter subunit G"
     gene            701..946
                     /locus_tag="PKH07_01860"
     CDS             701..946
                     /locus_tag="PKH07_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013905863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4040 domain-containing protein"
                     /protein_id="HNT33721.1"
                     /translation="MIQGALIVLSFACAVAAIALKDLLSSAIALAGFGLVISLLFYVL
                     HAPDVALTEAAVGAGISTVILVLTINRIRKNQGDAEP"
     gene            943..1464
                     /locus_tag="PKH07_01865"
     CDS             943..1464
                     /locus_tag="PKH07_01865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MnhB domain-containing protein"
                     /protein_id="HNT33722.1"
                     /translation="MSQQNKVQAFWVRCRRRPLEPLSAIVVGTLVALLLILGTTTDPD
                     HDSPLLSFYQTTALENLGVINLVSGIYLGYRAYDTLGETVALILAVTAASFMCGPAPA
                     GRSAMPNLPKGDHTEHNDQTTKDHAKSRPAPAGGSAMPTPPKGVRTEHNDQATKDHAK
                     SRPAPNQEQENEG"
     gene            1461..1880
                     /locus_tag="PKH07_01870"
     CDS             1461..1880
                     /locus_tag="PKH07_01870"
                     /inference="COORDINATES: protein motif:HMM:NF015970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MnhB domain-containing protein"
                     /protein_id="HNT33723.1"
                     /translation="MRDSEILRIMARKIFPFMLLFGFYLIAYGHISPGGGFQGGVVLG
                     SSVILLCLCYGLESVEKMLPGSGSGLAECLLMIALIAVGLLGIWARGTFLGNIVWHEW
                     DEPLPRAGTILMLDILIGLKVGAGMTALFYHMARDER"
     gene            1887..2222
                     /locus_tag="PKH07_01875"
     CDS             1887..2222
                     /locus_tag="PKH07_01875"
                     /inference="COORDINATES: protein motif:HMM:NF012636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter subunit C"
                     /protein_id="HNT33724.1"
                     /translation="MAVWGSVLLLGFGAYAFIVMDNLIKKVFAMNIISSATVLFIIAQ
                     GYKPGATAPILADSPLPMVDPLPQALMLTAIVINLCMTALALVFIIRLFESERTLNES
                     QLDQQLKDQ"
     gene            2224..3852
                     /locus_tag="PKH07_01880"
     CDS             2224..3852
                     /locus_tag="PKH07_01880"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HNT33725.1"
                     /translation="MIIYLIVVPLLGGFLMLLLGHIAKGSRMVAPLTVLMLLGTAGVL
                     LSLVPSMQLGQVIEYDVGGWPDPIGITLKMDGMAFLGSCLGMVIMLLAAFYAMAERRY
                     GAIFFGILLILAAAMQGVLLTNDLFNLYVLLEIVSLCTYFLVAYHKQTRSMKAALDYL
                     LVSSLAFCLYLFGVGLIYRATGVLGFQSLSEMFSQPGWVKDSSLTLAMALILTGAGVK
                     AVFFPLHTWLPDAHAQAPTPISALLSGVMIKISFLAVWRTLDAFCADHFAPALMVIGL
                     TSALWGGCMAVVQTDMKRLLACSSISQMGLIITAFAVGTSVGRMASLLHVVNHACFKS
                     LLFFCAGIIITKTGKRDMRQIAGLATGEPLLVVCLLVGAGAISGVPGFGGFVSKGWVA
                     SSLAGKPLAIVAVTLAGILTVASMARLVWTLWGRQEQPCQDNTSCQVRLWIPAFTLTV
                     LCLVLGVLPRLPIQWVRHALDCTDAPDLSVYSMSRISGSLVVVVLGLSLFFALRTPTG
                     TRISERVRNLDANLNIKLLLVIVAAVAYYLLAAF"
     gene            3862..5115
                     /locus_tag="PKH07_01885"
     CDS             3862..5115
                     /locus_tag="PKH07_01885"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNT33726.1"
                     /translation="MRILHIIHRYPPAQGGSEKYFEEISKRFAQRGDSVEVWTTDADQ
                     LERFWIRDRKRLPAGVSMDEGVTVRRFRTVVVPQHARVQRLLCLLPSQNLRRLFHFPS
                     PLVPGMIRRAAMEPLQFDVVHATALPYTSILYAAWIAARRSAARLCYTPFMHIGEKHS
                     DEVRKWYARRDQIQLLQKADAIFVQTPTERDFLISCGLDGSKCLITGVGVNPEEIVPP
                     KPIENLMCERYGLRPPYVFTLGAKSLDKGTLSVIEAMARVWQQHPEVTLVLAGDEMSD
                     FQEYWGTVPSESKKSILNLGRISDAEKVDLIRQGLIYLNPSRTDSFGITFLEAWLWEK
                     PVIGARAGGVVDVISHGEDGFLVEFGCASEIASKILELIRNPSMQASMGAKGKTKTLS
                     KYTWESIFESIAKRYQSVVKSLRHA"
BASE COUNT         1051 a         1431 c         1503 g         1312 t
ORIGIN      
        1 gacgggggat aatctgctca tccactggct ggatgccaaa acaacacaca aacgccttgc
       61 gggacacctc atcaagggga gtatggaaga ctggatcagg agaatctgga catgagcttt
      121 cttacgttca tcacgttcac actccttctg atctcgttgg gaagccttta tcgggtcatc
      181 ttcgggccca cgccccacga ccgactggtg ggactgaatc ttctcgtcgc cctggttacc
      241 ggcgtcatgg tgctgcttgc agtcttgtac aagcgggaca tctatctcga tgtcgccctg
      301 gtctatacga tgttgggatt tatcagcgtc atcgctgtca ccaagcacct gaagggaaaa
      361 ggattgagcg aatgacaatt ctggcgcata tcggtttggt tgcgggcatc tttttcgctc
      421 tcctgggcgt ggttggaacg ctgcggtttc cagatgtcta cacacgactt caggcgtctt
      481 caaagtgctc tacaacagcg ctcctggtgc tgtacgtggc agccatgttg catgcgtgag
      541 atctaagaat ggccgtgcgc atcttcgcgc tgcttctgtt cactctggtg accgcacctc
      601 tcggctctca tgcgattggg cgcagcgcct atgaatgcgg tatccttccc tggcgcggca
      661 agcgcgagcc gcgatcccaa ccgatggaga acagtgaatc atgattcagg gtgccttgat
      721 cgtgctctcg tttgcctgcg cagtggctgc gattgccctc aaggatcttc tgagttcggc
      781 cattgccctg gcaggtttcg gtctggtcat ctcactgctg ttttatgtgc tccatgcgcc
      841 ggacgttgct ttgacggaag ccgcggtcgg ggcaggcatc agcacggtga tcctagtgct
      901 gaccatcaac cgaatacgca agaaccaggg agacgcggaa ccatgagcca acagaacaag
      961 gttcaggcgt tctgggttcg atgcaggcgg cgtccgctgg agccactgtc ggcgattgtt
     1021 gttggaacac ttgtcgcact gcttctgatt ctgggaacga cgaccgatcc ggatcacgac
     1081 tcgcctctgc tgagcttcta ccaaaccaca gccctcgaaa acctaggcgt cattaacctg
     1141 gtctcgggca tctacctggg ctatcgagcc tacgacacgc tgggcgagac ggttgctctg
     1201 atcttggcgg tgaccgccgc ctccttcatg tgcgggcctg cgcccgctgg caggtcggct
     1261 atgccgaatc tcccgaaagg tgatcacacg gaacacaacg accaaaccac caaagatcac
     1321 gccaagagtc ggcctgcgcc cgccggcggg tcggctatgc cgactccccc gaaaggggtt
     1381 cgcacggaac acaacgatca agccaccaaa gatcacgcca agagtcggcc tgcgccaaat
     1441 caagaacagg agaacgaagg atgagggact cagagattct ccgaatcatg gcgcgcaaga
     1501 tattcccctt tatgctcctg ttcggtttct atctgattgc gtatgggcat atctctccgg
     1561 gcggtggctt tcaggggggc gttgttctgg gctcttcggt catactgctg tgcctgtgct
     1621 acggtctgga gtctgtggag aagatgctgc ccggttccgg ctcgggtctg gcggagtgtc
     1681 tcctgatgat tgccctgatt gccgttggac tgctgggcat ctgggcgcga ggtactttct
     1741 tgggaaacat tgtctggcat gagtgggatg aaccgctgcc acgcgctggg accatcctta
     1801 tgctggacat tctgatcggg ctcaaagtag gcgcgggcat gacggcactt ttttatcaca
     1861 tggcgcgtga cgaaaggtaa tcagcaatgg cggtctgggg aagtgtgctg cttctgggat
     1921 ttggggcata cgccttcatt gtcatggaca atctcatcaa gaaggtgttt gccatgaata
     1981 tcatcagcag cgccaccgtg ctcttcatca ttgcccaggg atacaaaccc ggcgcaacgg
     2041 ctcccatcct ggcggacagc ccgctcccca tggtggatcc acttccgcag gcactcatgc
     2101 tgaccgccat cgtcatcaat ctctgtatga cggcattggc tttggtgttc atcattcggt
     2161 tgtttgagag cgagcgcacg ctcaacgagt cccaactcga tcaacagctt aaggatcaat
     2221 gaagtgatca tctatctgat cgtggttcca ctacttggcg gtttcctgat gctgctgctg
     2281 ggacatattg ccaagggctc acggatggtg gctccactga cagttctgat gcttctgggt
     2341 acggcaggag tgcttctgtc tcttgtgcct tccatgcagt tgggacaggt tatcgagtat
     2401 gacgtcgggg gatggccgga tcctattggt atcacactca aaatggacgg aatggccttc
     2461 ctgggctcgt gtctggggat ggtgatcatg ctgcttgcag cgttctatgc catggcagaa
     2521 cgacggtatg gagccatctt cttcggaatt ctgttgattc tggccgctgc catgcagggg
     2581 gtgcttctga ccaacgatct tttcaacctt tatgtgctcc ttgagatcgt ttccctttgc
     2641 acctattttc ttgttgccta ccacaaacag acccgatcca tgaaggcagc gttggactat
     2701 cttcttgtca gcagtcttgc cttctgtctc tatcttttcg gcgtggggtt gatctaccgc
     2761 gctacgggcg ttcttggatt tcagagcctg tcggaaatgt tctcacagcc aggatgggtg
     2821 aaggactcca gtctgactct tgcgatggcg ttgatcctga ctggagctgg cgtcaaggcg
     2881 gtgttcttcc ctctgcacac ttggctgccc gacgcccatg cccaggcacc aacaccgata
     2941 tcagcccttt tgtccggcgt gatgatcaag atttcctttc tcgccgtctg gagaacgctg
     3001 gatgccttct gcgccgacca ttttgcacca gcactcatgg ttattggact gacatccgcg
     3061 ttgtggggtg gctgtatggc tgttgtccag acggacatga aaagactgtt ggcatgcagc
     3121 agcatttccc agatgggctt gatcatcacg gcgtttgctg ttggcacctc tgttggtcgg
     3181 atggcatcgc tattgcatgt ggtgaaccat gcctgtttca agagccttct gttcttttgt
     3241 gcggggatca tcatcaccaa gacgggaaag agagacatgc gacagatcgc cggcctggcc
     3301 acaggagagc cgttgctcgt tgtctgcttg cttgttggag caggtgccat ctccggcgtt
     3361 ccaggattcg gaggcttcgt cagcaaaggc tgggtggcct catctctggc tggaaaacct
     3421 ctggccatcg tggcagtcac gcttgcaggc atattaaccg tggcctcaat ggcacggctg
     3481 gtctggacgc tttggggacg tcaggaacaa ccgtgccagg acaatacttc ttgccaggtg
     3541 cgcctctgga ttcccgcatt caccctgacc gtgctctgtc tcgtgcttgg cgtgctgccg
     3601 aggctgccca tacaatgggt gcgccatgct ctggactgta ccgatgctcc tgatctctcc
     3661 gtttactcca tgtctcggat cagcggctcc ctggtggtgg ttgttctggg tctctcgctc
     3721 ttttttgcgc tccgaacacc gacaggaacg aggatttccg agcgggtgag aaacctcgac
     3781 gcaaacctca acatcaaact gctgttggtc attgtggcag cggtggcata ttatctcctg
     3841 gcagcgtttt aggaactgcg tatgagaatt ctccacatca ttcatcgtta tcccccggca
     3901 cagggcggct ccgagaagta tttcgaggag atctcaaagc gtttcgcaca acgaggggat
     3961 tcggtcgagg tttggacaac ggacgctgac caactcgaac gcttttggat acgagataga
     4021 aagaggcttc cggccggtgt cagcatggac gaaggcgtta ccgttcgccg attccgaacg
     4081 gtggttgttc cacaacatgc acgcgtccag agactgttgt gtctccttcc ttcacaaaac
     4141 cttcggaggc tgtttcattt tccatcgcct ctggttccag ggatgatccg tcgtgcggct
     4201 atggaaccac tccagttcga tgtcgtgcat gctaccgcgc ttccttatac ctcgattctc
     4261 tacgcggcct ggatcgccgc tcggcgatcg gcggcacgcc tgtgttacac cccgttcatg
     4321 catattggag agaagcactc cgacgaagtg cggaaatggt atgcgcgacg tgatcagatt
     4381 cagctgcttc agaaagcgga cgcgatcttt gtccagacac caacggagcg cgattttctg
     4441 atttcatgcg gtcttgatgg ttccaaatgc ttgatcacag gtgttggggt gaatccggaa
     4501 gagattgtcc cgcccaaacc gattgagaac ttgatgtgtg agcgctacgg actgcgcccg
     4561 ccctacgttt tcacgcttgg cgcaaagagt ctcgataagg gaacgctctc ggtcatcgag
     4621 gcgatggctc gggtctggca gcagcatccc gaggtcacgc tcgtgctcgc cggtgatgag
     4681 atgtctgatt tccaggagta ttgggggact gttccgtcag aaagcaagaa aagcattctg
     4741 aatttgggac gtatctcgga cgcggagaag gtggatctga tacgacaagg cctcatctat
     4801 ctaaatccgt ccagaacgga ctcatttggt atcacttttc tcgaagcgtg gctctgggag
     4861 aaaccggtta tcggagcacg ggcaggtgga gttgtggatg tgatcagtca cggcgaggac
     4921 ggatttctcg ttgagtttgg ttgtgcctca gagatcgcgt cgaaaattct ggaactgatc
     4981 cggaatccat ctatgcaggc atccatgggc gccaaaggca aaacaaagac gctctccaag
     5041 tacacatggg aatccatttt tgaaagcatt gcgaagcggt accagagcgt ggtcaagagt
     5101 cttcgccatg catagggtgt gaatccgcag tcttcttctt tgattcgtga cggtgcacca
     5161 accggcgagc aaatctgaag aggaattgtc agtgtgaaga caatgaggat ccccaatgcc
     5221 gccccccccc ggagatggat ctacagtcga tccgccatag gttccgcttc cagaggcgct
     5281 cccccagcat ccgatgc
//
LOCUS       DAOQUC010000065         5294 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_17158_length_5294_cov_2.608895, whole genome shotgun sequence.
ACCESSION   DAOQUC010000065 DAOQUC010000000
VERSION     DAOQUC010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5294
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17158_length_5294_cov_2.608895"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="PKH07_01890"
     CDS             <1..241
                     /locus_tag="PKH07_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014157160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HNT33727.1"
                     /translation="QMLKKNCAGTVFIHLRGDRDLILSRMAHRRDHFMPAQLLDSQLG
                     ILEEPEDCVTVDISGTPEQIVDAIVQHLNDRRLLR"
     gene            238..1686
                     /gene="gnd"
                     /locus_tag="PKH07_01895"
     CDS             238..1686
                     /gene="gnd"
                     /locus_tag="PKH07_01895"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010416288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating NADP(+)-dependent
                     phosphogluconate dehydrogenase"
                     /protein_id="HNT33728.1"
                     /translation="MNRADIGMIGLAVMGENLVLNMESKGFTVAVYNRTVSKVDDFLA
                     NRAKGKRIVGCHSLVELCSSLSKPAKVMLMVKAGTPVDEFIAQLLPNLQKGDIIIDGG
                     NSHFPDTIRRCRQLQEKGIHYIGTGVSGGEEGALLGPSIMPGGSPEAWPTVRPIFQAI
                     AARTPDGEPCCDWLGNDGAGHFVKMVHNGIEYGDMQMICETYHLMKQALGMTNDEMSA
                     AFSEWNDAELNSYLIEITRDILAYKENGKHVLNTILDAAGQKGTGKWTAVAALDLGQP
                     LTLIVEAVFARALSAMKDQRVQASKSFSDPQPLLVLDRERFLDDLRKALYAAKITSYA
                     QGFMLMRAAAEEYAWNLDYGSIASMWRGGCIIRSVFLTKIKEAFGRNPRLANLMLDSF
                     FKQIIESSQQPWRRVIASAVTLGIPVPAMSTALAFYDGYRSEWLPANLLQAQRDYFGA
                     HTYERIDRPRGESFHTNWTGRGGDTSASTYTV"
     gene            1697..2458
                     /locus_tag="PKH07_01900"
     CDS             1697..2458
                     /locus_tag="PKH07_01900"
                     /inference="COORDINATES: protein motif:HMM:NF014571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAC(3) family N-acetyltransferase"
                     /protein_id="HNT33729.1"
                     /translation="MTNSISKTEILEGLRQLGLRRGSVVLVHSSLSRFGRVVDGADTV
                     IDALLESVGPRGTVMVPTLTGSPELSPSNPPVFDPVNTACWTGVIPETFRKRPGAIRS
                     LHPTHSVAAIGFEADSLTCEHRFSITPCDDLSPYGKLARHPKGYVLLLGVGHECSTLF
                     HHIEESAGVDYHIQTDFTAAQIVSGDHAIVRHYLLHQYGTPRNFAVMEQIFIERGIQR
                     TATIGSASVRLIDAPQMVATTLRCLRSSRRFLCRP"
     gene            2455..3024
                     /locus_tag="PKH07_01905"
     CDS             2455..3024
                     /locus_tag="PKH07_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006003269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HNT33730.1"
                     /translation="MSTMVILGIAFGLAMDAFAASIACGVILCGVSPRQIFRLSFHFG
                     LFQFAMPVIGWLAGLSIIGWVESFDHWIAFVLLGFIGGKMIWESFHSFEERSQSGNDP
                     TRGLSLVSLSIATSIDALAVGLVFATEKVRILYPCIVIGLVAGIMTALGMAIGCRIGA
                     RFGSRMERIGGAVLIVIGLKILFSHVSAS"
     gene            complement(3041..4420)
                     /gene="creD"
                     /locus_tag="PKH07_01910"
     CDS             complement(3041..4420)
                     /gene="creD"
                     /locus_tag="PKH07_01910"
                     /inference="COORDINATES: protein motif:HMM:NF008712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HNT33731.1"
                     /translation="METIVKSESTLELFFQRQAMTFKLAWIAFLVLVLLIPVGMVKSL
                     LHERTGRRDAAVSEMTFSWAGEQTIAGPVLVIPYRYVVKNWKEETVNGKPERFEVEET
                     LTAEAFFLPDSMSIEGSIEPSLLHRGIYDAVVYNGALEISGQFSPPDFTELDIDPVNV
                     LWDKSSVTFAIQDLRGAAEMLTVTIGDTPYEFKPGSKLTGYSSGIIARLPNLSRDSVS
                     DYRMHLEIKGSRGIYFTPVGKQNRVKLSSPWTAPSFQGSYLPTTRGVTDDGFEALWEV
                     SWYGRGYPQQSTNRDSSCALNADRLSGSRFGVNFIVPLDSYRMVERSIKYAALFIILV
                     FAAFFIFESFYQLKIHTIQYTLIGVALCLFFLAVLSLSEFFHFFYAYWTGALASALLV
                     ILYSLSVLKRAVRAATVGVILLATYTYLYVILQLQDYSLLFGTVGLFAALAVVMYVTN
                     KMDAVKRMP"
     gene            complement(4497..4892)
                     /locus_tag="PKH07_01915"
     CDS             complement(4497..4892)
                     /locus_tag="PKH07_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HNT33732.1"
                     /translation="MDKHHHEDHDRGRFAYEGLDRVLHEKARLGILTALVTHREGLLF
                     TQIRDLCALSDGNLSRHIQALEEAGLVEVWKGFRAKKPQTLCRISKNGLCRFHEYLEE
                     LERVIRDASVAGEEDPSTESAMGKRLVPI"
     gene            complement(4873..>5294)
                     /locus_tag="PKH07_01920"
     CDS             complement(4873..>5294)
                     /locus_tag="PKH07_01920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33733.1"
                     /translation="LISVAGVVGNYLHDNDRNNRRRTFVVFGQLAPSLCAAVVTTVAF
                     LSSEDKTAVAFLPGIWSLLYGLGLLASRPFLPRMIGWGALFYLLCGCFLLRRAALMGI
                     AGLSPWEVALPFGLGQVLGAAILYWNLERRNHGQTSS"
BASE COUNT         1314 a         1404 c         1333 g         1243 t
ORIGIN      
        1 acagatgttg aagaagaact gcgctggaac tgtcttcatt catcttcgtg gagaccgtga
       61 cttaatcctt agtcgcatgg cacacaggag agatcatttc atgccagcgc aattgcttga
      121 cagtcagttg ggcatattgg aggagccaga ggattgcgtc accgttgata tctctggtac
      181 tcccgagcag atcgtggatg ctattgtaca acatctgaac gataggagat tgcttagatg
      241 aacagagcag atatcggtat gatcggactg gcggttatgg gagagaacct cgtcttgaac
      301 atggagagca aggggttcac tgtggctgtc tacaatcgaa ccgtttccaa agtggacgat
      361 ttccttgcca accgagcaaa aggcaagagg attgttggtt gccatagcct tgtggaactg
      421 tgctcatctc tgtctaaacc tgccaaagtc atgctcatgg tcaaggcagg cactccggta
      481 gatgagttta ttgctcaact gctcccgaat ctccagaagg gcgacattat cattgatggt
      541 ggcaactcac actttccgga taccatccga cggtgtcggc aactgcagga gaaaggtatt
      601 cactacatcg gaactggtgt ctctggtggt gaagaaggtg cactgctcgg cccttcgata
      661 atgccaggtg gttccccgga agcatggccg actgtaagac ccattttcca ggctatcgcg
      721 gcgcgcaccc ctgatggaga accctgttgt gactggcttg gaaacgacgg tgccggtcac
      781 ttcgtcaaga tggttcacaa cggcatcgag tacggagaca tgcagatgat ctgcgagacc
      841 tatcatctca tgaagcaggc acttggcatg acgaacgatg agatgagcgc tgcgtttagc
      901 gaatggaatg atgccgagct aaactcctac cttatcgaga tcacgcgaga tatccttgcc
      961 tacaaggaaa atggaaagca tgttctgaac acaattcttg acgctgccgg acaaaaaggg
     1021 acaggaaaat ggactgccgt ggctgcgctc gatctgggtc aaccattgac gctcattgtt
     1081 gaagcagtct tcgctcgcgc tctctccgct atgaaagatc agcgagttca ggcgtcaaaa
     1141 tccttttcag atcctcagcc attgttggtt cttgacagag agagattcct tgacgatctc
     1201 cgcaaggcgc tctacgcggc aaaaatcacc agctatgcac aaggttttat gttgatgcgc
     1261 gccgccgctg aggaatatgc atggaatctc gactacggaa gcatcgcctc gatgtggcga
     1321 ggtggctgca ttatccggtc tgtgttcctt actaagatca aggaagcatt tggcaggaac
     1381 ccacgtctgg cgaatctgat gctggattca ttcttcaagc agatcatcga atcgtctcag
     1441 cagccctggc gccgcgtgat cgcatctgct gtcacattgg gcattccagt cccagcgatg
     1501 agcaccgcac ttgccttcta cgacggttac cgtagcgaat ggctgccagc gaatctcctt
     1561 caagcgcaga gggactactt cggagcacac acctacgaac gcattgaccg tccacgaggt
     1621 gagtctttcc acacaaactg gactggcagg ggtggcgaca cctccgcatc aacatacaca
     1681 gtgtgagagt tgcgatatga cgaattccat cagtaagacg gaaatcctcg aaggcttgcg
     1741 ccaattgggc ttaaggcgtg gctccgtcgt tctagtgcac agttccctaa gccgcttcgg
     1801 acgagttgtg gatggagcag atacggtcat agatgcattg ctggagtcag tcgggcccag
     1861 gggcactgtc atggttccaa ccctcactgg atcgccggaa ctctcgccat ccaaccctcc
     1921 ggtcttcgac ccggtcaaca cggcttgttg gacaggtgta ataccagaaa catttcgaaa
     1981 gcgacccggt gccatccgaa gtctccaccc aacgcactct gttgccgcca tcggctttga
     2041 agccgattca ctgacctgtg aacatcgctt ctcgattaca ccgtgcgatg acctttcacc
     2101 ctatggcaaa ctggcaagac atcccaaagg ttatgttctt ctcctcggtg tcgggcacga
     2161 atgcagcact ctctttcatc acattgaaga gagcgccggt gttgactacc acatacaaac
     2221 agatttcacg gcagcacaga ttgtcagcgg cgatcatgcc atcgtcaggc actacctact
     2281 acaccaatat ggaaccccga ggaattttgc agtcatggaa cagattttca tcgaaagagg
     2341 aatccagaga actgcgacaa ttggaagtgc ctcggtgcga ctcattgacg caccacagat
     2401 ggtggcgacc acccttcgat gtcttcgcag cagccggagg tttctctgta ggccatgagt
     2461 acgatggtga ttctcggtat tgcttttgga ctcgcgatgg atgccttcgc cgcttcaatc
     2521 gcctgtgggg tcattctctg cggagtttcc ccaaggcaga tattccggct ctcttttcat
     2581 ttcggcttgt ttcagttcgc aatgccagta attggatggc tcgcaggctt gtccatcatc
     2641 ggatgggtcg aatcattcga tcactggatc gcgtttgtcc tgttgggctt catcggcggc
     2701 aaaatgatct gggaatcgtt ccacagtttt gaagagcgtt cacagtcggg taacgacccg
     2761 actcgtgggc tttcactggt ttctctctcc atcgcgacga gcattgatgc ccttgctgtc
     2821 ggcttggttt tcgctacgga gaaggtgaga attctctatc cctgtatcgt tatcggtctc
     2881 gttgccggaa ttatgacggc gctcggaatg gccatcggct gtcgcatcgg agcacggttt
     2941 ggcagccgta tggagcgcat cgggggcgcg gtgctcatcg tcattggcct gaagatcctc
     3001 ttctcacatg tctctgcatc atagccttgt gcatttcgtg tcatggcatt ctcttgactg
     3061 catccatctt gttagtcacg tacataacaa cggccaacgc tgcaaacaag cccaccgttc
     3121 cgaacaacaa agaatagtcc tgcaattgaa gtatcacgta gaggtaagtg tacgttgcca
     3181 agagaattac tccaacggtg gctgctcgaa ctgctctctt gagtacgctc agactgtaga
     3241 gaatcacgag cagtgcggaa gccaatgctc cagtccagta agcataaaag aaatgaaaga
     3301 actctgacag agacaatacc gccagaaaga acagacacaa cgccaccccg atgagggtgt
     3361 actggattgt gtggatcttc agctggtaga aggattcaaa gatgaagaac gccgcaaata
     3421 ccaagataat gaacagcgca gcgtacttga tggagcgttc gaccattcga tatgaatcca
     3481 agggtacgat gaagttcacc ccaaatctcg atccggacaa cctgtccgca ttcaaagcac
     3541 aagacgaatc gcgattcgtg ctctgctgag gataccctct tccgtaccag gatacttccc
     3601 acagcgcctc aaaaccatca tccgtcactc ctcgtgtcgt tggcaggtat gaaccctgga
     3661 aactcggggc agtccaagga gaggagagtt tcacccgatt ctgctttcca acaggagtga
     3721 agtaaattcc acggctccct ttgatctcca aatgcatccg atagtccgag actgaatctc
     3781 gtgagagatt gggcaggcgg gctatgatgc ctgacgagta gccggtaagt ttggaacccg
     3841 gcttgaactc atagggcgtg tctccaatcg taaccgtcaa catctcagca gcgccgcgca
     3901 agtcttggat ggcaaacgtt acagaagact tgtcccacaa aacattgaca ggatcaatgt
     3961 cgagttccgt aaagtccggc gggctaaact gaccggagat ctcgagagca ccattgtaga
     4021 caaccgcatc atagatacct ctgtgaagaa gacttggttc aatgctccct tctatggaca
     4081 tgctgtcggg aagaaagaag gcttccgctg tgagagtctc ctcaacttcg aatctttcag
     4141 gctttccatt cactgtctct tccttccagt tcttgaccac atatcggtat gggatgacca
     4201 ataccggtcc agcgatggtc tgttccccag cccaggaaaa ggtcatctcc gagaccgctg
     4261 catcgcggcg ccccgtcctc tcatggagaa gagacttcac catcccaact gggataagca
     4321 gaaccaagac cagaaaggcg atccaagcta acttgaaggt cattgcttgt ctctgaaaga
     4381 acagttcaag agtgctctct gactttacga tcgtttccat cttaccgctc cttatgcttt
     4441 ctatacccgc gtactttact gtacagagtt aaaagagaaa agaaaagtgt tccgcgtcag
     4501 atcggaacca gtctcttgcc catcgcactt tcggtggacg ggtcttcttc gccagccacc
     4561 gaggcatcgc gaatgaccct ctcaagctct tcgagatact cgtgaaagcg acagagtccg
     4621 ttcttgctga ttctgcaaag agtctggggc ttctttgctc gaaacccctt ccagacttca
     4681 acgagtccgg cttcttctaa ggcctggata tgacggctaa ggttgccgtc actcaaggcg
     4741 caaaggtctc taatctgagt gaacagaagc ccttcacggt gtgtcacgag agccgtcagt
     4801 ataccaagcc gagccttctc atgaagcaca cggtcaagac cttcgtatgc aaaccgtcct
     4861 ctgtcgtggt cttcatgatg atgtttgtcc atgatttctc ctttcaagat tccagtaaag
     4921 gatcgctgcc cccaaaacct gacccaaacc aaatgggagt gccacttccc aaggagacag
     4981 tcctgcgatg cccattaaag cagcgcgtct caacaagaag cagccacaga gtaagtagaa
     5041 gagagccccc caaccaatca tcctcggcag aaacggcctc gatgccagca aaccgagtcc
     5101 atatagcaat gaccagatac caggaagaaa cgcgacagcg gtcttatctt cggatgagag
     5161 aaacgcaaca gtggtgacca cagcggcaca aagactggga gccaattgac caaagacgac
     5221 aaacgtcctt ctgcgattgt ttctgtcatt gtcgtgcagg tagtttccta ccactccagc
     5281 cacagagatc agga
//
LOCUS       DAOQUC010000066         5279 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_17229_length_5279_cov_2.991194, whole genome shotgun sequence.
ACCESSION   DAOQUC010000066 DAOQUC010000000
VERSION     DAOQUC010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5279
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17229_length_5279_cov_2.991194"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..321)
                     /locus_tag="PKH07_01925"
     CDS             complement(<1..321)
                     /locus_tag="PKH07_01925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33734.1"
                     /translation="MFHRVKSVQAKISFWAGVFLFLTSALIVGFSARMMYKEAVKQRE
                     TALEDGKKKLLEQARNKARELESRLLDPYYSVQTINQIASGVKRDDLAGKHGMDTMII
                     DRPVL"
     gene            complement(347..3478)
                     /locus_tag="PKH07_01930"
     CDS             complement(347..3478)
                     /locus_tag="PKH07_01930"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015773086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HNT33735.1"
                     /translation="MHAEDETLLMYIEESREHFATIETDLLTIEEAGANIDDQLVNKV
                     FRAAHSVKGGAGFFGLAKIRDLAHRVENVLDMVRSKELVPSPEVVNILLVSFDQLRSM
                     IDNYKESNDVDITEFVTALTGLASSNLPQEKKASLSTPAAIKTTDGRDLFSATEFDIQ
                     HAVDKGNYVYIAQVDLLHDIQRKGKSPLALFKDLIACGVVLDCVFDVDSLGSLEGPLH
                     REFPLMILYGTVVDPSIAHTLMDVDESKVNLIEKPRSAQIEEELSEDKQEIQTSTPSK
                     PADHKPERNLPRVPAQQKAPSEPSPTSQDTSLRVQVQLLEALMNLAGELVLSRNQLLE
                     ALGIGETRAVQASAQRINLVTSELQEAIMQTRMQSIGNIFNKFPRVVRDLAKSLGKEV
                     RLDLSGKEVEMDKTIIEGLSDPLTHMVRNSVDHGIETPDVRKAQGKDPVGQVQLSAYH
                     EAGQVVIEISDDGKGIDVDRIAAGAVSRGLMTQEQVRAMSKRELLSLIFLPGMSTAEK
                     VTDVSGRGVGMDVVKTNLDRLGGTVEIETAVGGGSKFRIRLPLTLAIIPCLLVSCENQ
                     RFAIPQVGVQELIRIPVADIQKRIETVGEAQVLLLRDTLVPIVHLAEVLGIERTYYDP
                     KDGLRKPDRRENLADRRSRKQGLFESAEIPSDSEQETSENRLGSDRRYRADSDLNIVI
                     VSAGPITYGLVVERLHETVEIVVKPLGKHLKSLKEYAGATIMGDGRVALILDVGGLAS
                     IASLNSVGGSTRAQELHDARSAESLADTQTLLLFRNSAEEPASVPLNLVKRIERIEAA
                     QVQTLAGQRIMQYGGVNLPLFTLGDVANVASFDLSGELVVLISQVSNREVGVLAQGPL
                     DVVEAALIQEQTHLRQDGILGSIIINGVSTILVDIFELVECAKPDWAAERKDQSPTKT
                     TDAGLITILLAEDSEFFRNQVTRFLEGDGYKVLPTEDGEFAWECLLKNADQVNLVVTD
                     IEMPRLNGLELTRRIRSESRFSQLPIVGLTSLAADEDIVRGKTAGINEYLIKLDKENL
                     LKSIGRLVSEIRK"
     gene            complement(3490..3801)
                     /locus_tag="PKH07_01935"
     CDS             complement(3490..3801)
                     /locus_tag="PKH07_01935"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNT33736.1"
                     /translation="MSETIIREGNRATVKPTQRFTASLSEQLKPELKDLVDQGVAEVI
                     FDLLGVDDIDSISIGLIVSTHNSLKKLGGVVRVINVCDDIISLFKTMRLDQHFTIQGV
                     D"
     gene            complement(3798..4241)
                     /locus_tag="PKH07_01940"
     CDS             complement(3798..4241)
                     /locus_tag="PKH07_01940"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNT33737.1"
                     /translation="MNVYQTKVRPGEITGVFTASFECVDVFCRQVRDLLPDGFSKSAR
                     FEITLLLREALNNAIVHGSNRSLEQSVCCTVTVQKPWVTITVQDQGEGFEWNERLHRQ
                     GSTADCQHPMTGIDIMKSMADEVQYNTKGNTISLRKKILSLEEGQ"
     gene            complement(4669..>5279)
                     /locus_tag="PKH07_01945"
     CDS             complement(4669..>5279)
                     /locus_tag="PKH07_01945"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNT33738.1"
                     /translation="QYMPLGSDSYKRTDARLVLATNRNLDSMQKSGSFRKDLYYRLCT
                     HRVRIPRLRDRLDDLPLLLDHFMHEASQQMGKKTPKAPKELITLLSNYNFPGNVRELR
                     AMVFDAVSQHSSMMLSMESFKEHIYGTSDGEMDLTLGKETSDSGNLSFGPKLPTLEEA
                     KQLLIEEALRRTGGNKSLAATMLGVTRQALILRERRKNSDTL"
BASE COUNT         1209 a         1478 c         1256 g         1336 t
ORIGIN      
        1 cagcactgga cgatcgataa tcatagtgtc catgccgtgc ttgccagcaa ggtcgtcccg
       61 cttgacaccc gatgcgatct ggttgatggt ctgtaccgaa tagtatggat caaggagacg
      121 gctttcaagc tcacgagcct tgttcctggc ttgctccaag agcttcttct tcccgtcctc
      181 caaggctgtc tcgcgctgct tgacagcctc cttgtacatc atccgcgccg aaaacccgac
      241 gatcaacgct gacgtcaaga agaggaatac acccgcccag aacgatatct ttgcctgaac
      301 cgacttcacc cgatgaaaca taatagccct cctaaactct ttgctgctat ttcctgattt
      361 cggacaccaa gcgtccaatg cttttcaata gattctcttt gtccaacttg atcagatatt
      421 catttatgcc tgccgtcttc ccacgtacaa tatcttcatc tgctgccaga gaggttaatc
      481 cgacgatcgg cagttgtgag aaacgtgact cggatctgat tctccgtgtc aactccagac
      541 cattcagacg cggcatttcg atatccgtca caaccagatt cacctggtct gcattcttca
      601 gtagacactc ccaggcaaac tcaccgtctt cagttgggag gactttgtac ccgtcaccct
      661 caaggaatcg ggtcacctgg ttgcggaaaa actcagagtc ttcagccaac agaatcgtga
      721 tcaggcctgc atccgtggtc ttcgttggag actgatcctt ccgttcggca gcccagtccg
      781 gtttggcaca ttccacgagt tcaaaaatgt ccacaagaat tgtgctgacg ccgttgatga
      841 tgatggagcc gaggatgcca tcctggcgga gatgcgtctg ctcctgaatg agggcggctt
      901 cgaccacatc gagtggtccc tgggcaagta cccctacctc ccggttggag acctgggaga
      961 tcaggacaac caattcaccg gagagatcga acgatgccac attcgccaca tctcccaacg
     1021 taaacagtgg caagttcacc ccgccatact gcataatgcg ctgccccgcc aaggtctgca
     1081 cttgggcagc ctcgatgcgt tcgatacgtt tcaccaggtt caaaggaact gaggcaggct
     1141 cctccgcgga gtttctgaac agtagaagcg tctgggtgtc agcgagtgac tcggcacttc
     1201 gagcgtcgtg aagttcttga gccctggtac ttccaccaac ggagttcaaa gaggcgatcg
     1261 aggcaagccc gccaacatca agaatgaggg caacacggcc atcgcccatt atcgttgccc
     1321 cggcatattc tttcaggctc ttgagatgct ttccaagagg cttgacgaca atctctacgg
     1381 tttcatggag tctttcgaca accaagccgt aggtgatggg gcctgcagaa acgatgacaa
     1441 tattgagatc actgtcggca cggtatcttc gatcgctgcc caggcggttc tctgaggttt
     1501 cctgctcgga gtctgatgga atctcagcgc tctcaaagag accttgcttt ctactccgcc
     1561 gatccgcaag gttttctcgc cggtctggct ttcggagccc gtctttggga tcgtaatatg
     1621 ttcgctcaat tccgagcact tcagccaagt gaacaatcgg cacaagagta tccctcagca
     1681 gtaacacctg cgcctctccg accgtctcta tcctcttctg aatgtcggca accgggattc
     1741 ttatcaattc ctgcaccccg acttggggaa tggcgaatcg ctgattctcg catgacacca
     1801 aaaggcacgg gataatcgcg agtgtcaaag gtaaacgaat tctgaatttg cttccgcctc
     1861 ccacagcagt ttcgatttcc acggttccac caagtctgtc aagattggtc ttgacgacat
     1921 ccattcccac accccgtccg gagacatccg ttactttttc agcggttgac attcctggca
     1981 ggaaaatcag agaaagcaat tcgcgcttgg acatggcgcg aacctgctcc tgggtcatta
     2041 agcccctcga aacagcacca gcagcaatcc tatctacatc tatccccttg ccgtcgtcgg
     2101 agatctcaat gacaacctgc cctgcctcgt gataggcgct caattgcacc tggccaacgg
     2161 gatcctttcc ctgtgccttg cgcacgtccg gagtctcgat gccatggtca accgagtttc
     2221 gtaccatgtg tgtcaaggga tcgctcagtc cctcgataat cgtcttgtcc atctcgactt
     2281 ctttaccgga caggtccagt cggacttctt tgcccagact ctttgccaga tcgcgaacga
     2341 cgcgcgggaa cttattgaag atgtttccaa tgctctgcat ccgcgtttgc atgatggctt
     2401 cttgaagctc ggaagtcacg agattgattc gctgagcgct ggcttgcaca gcgcgcgtct
     2461 ctccgatccc gagcgcctca agcagctgat ttctgcttaa caccagttct cccgcaaggt
     2521 tcatcaaagc ttccagcaac tgtacctgga cccggagaga agtatcttga gacgtgggcg
     2581 atggttcact cggtgctttc tgctgggcag gtacccttgg cagatttctc tcaggcttgt
     2641 ggtcagccgg tttcgacgga gtagaggttt ggatttcctg tttgtcttcc gagagttctt
     2701 cttcaatctg ggcgcttcga ggcttctcga tgaggttaac tttggactca tcaacatcca
     2761 tcagtgtatg ggcgatactt gggtcaacaa ccgtgccata gagtatcatc aagggaaact
     2821 cgcgatgaag aggcccctcc agcgatccaa gggaatccac atcaaaaacg cagtcaagca
     2881 cgacgccaca ggctatcaag tctttgaaga gcgccagggg gcttttccct ttgcgctgaa
     2941 tatcatgcag gagatctacc tgtgctatgt aaacatagtt gcctttgtca acggcatgct
     3001 gaatatcgaa ctcggttgcc gagaagaggt ctcgaccatc cgttgtcttg atagccgcag
     3061 gagtactaag cgatgccttt ttctcttggg ggagattgga agaagccaga ccggtcaacg
     3121 ctgttacaaa ctcagtgatg tcaacatcgt tgctttcctt gtagttgtca atcatgctgc
     3181 gcagctgatc aaacgacacc agaagaatgt tcaccacttc cggagaggga acgagttcct
     3241 tggaacgtac catgtcaagc acgttctcaa cccgatgcgc caggtccctg atctttgcca
     3301 acccaaagaa gcctgctccc cccttcactg aatgtgcagc acgaaacact ttgttgacca
     3361 attgatcgtc aatgttggct cccgcctctt cgatggtcag gagatccgtt tcgatggtcg
     3421 caaaatgctc cctggactcc tcaatgtaca tcaaaagtgt ctcgtcttcg gcatgcatga
     3481 gccgctctcc tagtcgacgc cctgaatcgt gaaatgctga tccagtcgca tggtcttgaa
     3541 cagactgatg atgtcgtcac acacattgat gacccgaacc acgccaccaa gcttcttcaa
     3601 ggaattgtgt gtcgaaacga tcaatccgat gcttatcgag tcaatgtcat ccactccaag
     3661 aaggtcgaag atgacttcag cgacgccctg atcaaccaag tccttcagct caggtttcaa
     3721 ctgctcagac agactcgcag taaaccgttg agtcggtttg accgtcgccc tatttccttc
     3781 cctgatgatg gtctcgctca ttgtccttcc tccaaggaga gaatcttctt gcgcaaacta
     3841 attgtgttgc ctttggtgtt gtactgcact tcatcagcca tagatttcat aatatcgatt
     3901 ccggtcatcg gatgctggca gtcagcggtt gaaccttgcc gatgcagtcg ctcattccac
     3961 tcaaacccct cgccctgatc ttgaactgtg attgtcaccc agggtttctg gaccgtcacc
     4021 gtgcagcaga cggattgctc gaggcttcta ttggaaccat ggacgattgc gttgttcagt
     4081 gcctcgcgca gcaataaggt aatctcaaac cgagcagact tggaaaaacc gtcgggaagc
     4141 agatctcgaa cctgccggca aaatacgtcc acacattcaa aacttgccgt gaatactccc
     4201 gtgatctccc caggacgtac ctttgtctga tatacattca tgcttctgca gctcatggcg
     4261 caaccgcctg actcaacgag aacaaaatct gtcccacggt ttcggacagg tcagcacatt
     4321 cacgtgctgt cctgtgtggt gctggcttac caaagaaact gcctcctgcg aagtccgcct
     4381 tcctggtaca gcatcccaca ttgacattct cattcacttc ctattcttta caggcctgaa
     4441 tcctgttctg catggctatt tagcagggag tgagccaact cgtgaacatt tccataagtt
     4501 attgcataac aattagttat atgctgtcaa agagacatcg tcttgtttca ggtgggtgtc
     4561 aaagaagttg gcagaaagca agtcaaatga atcttaagtc atttgtatac agtcagttat
     4621 gcaccaagat agcatctcat cctgtcggcg tatttgacac cacccgcgtc agagtgtgtc
     4681 agaattctta cgtctctccc gaagaatcaa ggcctggcga gtaactccaa gcatcgttgc
     4741 cgcaaggctc ttgttgcctc cggtacgcct cagagcttcc tcaatgagca gctgcttggc
     4801 ctcctccaac gttggcagtt tggggccaaa agaaaggttg cccgaatcgc ttgtctcctt
     4861 ccccaacgtt agatccatct caccgtctga agttccgtag atgtgttctt tgaacgactc
     4921 catggaaagc atcattgagg aatgctgact gacggcatca aacaccatgg ctctcagctc
     4981 acgaacgttt ccggggaagt tgtagtttga gagcagcgta atcagttcct tgggagcctt
     5041 gggagtcttc ttgcccatct gctgagatgc ttcatgcatg aagtgatcca gcaggagtgg
     5101 cagatcatcc aaacggtccc tcagccttgg aattctgact cgatgagtgc acagccggta
     5161 atagaggtct tttctgaatg atcccgactt ctgcatggag tccagattgc gattggtcgc
     5221 caagaccaac cgtgcgtccg tgcgcttgta ggagtccgat ccgagcggca tgtattgcc
//
LOCUS       DAOQUC010000067         5263 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_17293_length_5263_cov_3.383449, whole genome shotgun sequence.
ACCESSION   DAOQUC010000067 DAOQUC010000000
VERSION     DAOQUC010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5263
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17293_length_5263_cov_3.383449"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1273)
                     /locus_tag="PKH07_01950"
     CDS             complement(<1..1273)
                     /locus_tag="PKH07_01950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33739.1"
                     /translation="MSTQSHEINPGIKMRFALTLLIACFLVAECVVSTAQALEDSTSA
                     LSIQERPMLLAQSSRDRSSDDDLFGDNRRSSTSYRSERQRSESTTTRNTGTTENSRST
                     DTSRRTSSTDQDRRRIEEERARRSSERDSEERTSTPSQPTSGDSGRSSDRRGDGRSRR
                     ESGSGQPGSGGLEIVGPSPDAPAPPSPPGARTATGQVQAAPSERAILYLSPSSNIVSV
                     GQRFVLNLNLENPKELRFDKLGVTLSYDPRFLAVVEPAGEEASAAVKSASNVLIQPNA
                     EYRLMVNRSLPDKGLAFYHVHMMDGDTRRFKGSFGQIQFEAVEPSSATTIRFVGRDAN
                     VSLAELDPLGLPWTYLEAKGEDILGQPKIEGDGVLSATVRVNPSGEPLSPYATLRNAR
                     KQQRDRIPQGSVALSIVPSMLSVRQGEPFSVR"
     gene            1720..2616
                     /gene="xerD"
                     /locus_tag="PKH07_01955"
     CDS             1720..2616
                     /gene="xerD"
                     /locus_tag="PKH07_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HNT33740.1"
                     /translation="MRPEGKYLQEFSDFLTVERGLSKRTREAYLIDLENFRQYCSGHG
                     LAIPKDIDCVEVSRYVLDLQASGRTPATVGRAQSSLRQFFRFLVHEKITDQDPTVHLR
                     SPKQIKHLPQFLTQEEMERLLAAPDATDPRGIRDRTMLEVLYATGLRISELLSLRIED
                     VDLRLGFVRTRGKGDKERIIPMHSQAVSLLQDYIENTRTVFDKGIGGTVVFLNPSGRR
                     LSRMGFWKILRKWGLKAGIAKSFSPHTLRHSFATHLLQGGADLRSIQELLGHSDISTT
                     QTYTHVEQNRLREVHRRHHPRS"
     gene            2871..4979
                     /locus_tag="PKH07_01960"
     CDS             2871..4979
                     /locus_tag="PKH07_01960"
                     /inference="COORDINATES: protein motif:HMM:NF027232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33741.1"
                     /translation="MSLKRYLIVVSVFCLWASLAGSLEWREFGPPGATLLNVVRGTGW
                     VYAFGRDQVYKSNDAGLTWQNMPQVPLVQGDYLVDGIVFGATEQAILAVRSSKGILRW
                     DVASNSWIEVNQGITPPTPPYGWQFIGISAATPASTRAFAGVSTGLFYSDDFGDSWHK
                     VSANYAFQGIVAAADGSVYYDSGSSQQTQPFRLESDLTTLTGFTMRPGLPQNRAIYGT
                     IAPNPENPLELLFVESVDNKAYYSTDRGETWSSPALGTVPQGATSAGWLNGTPIVFSE
                     SSAYSLNKQTQTWSLLWTRPVADQYKVIAANANTLLLWGLQTNIWRSTNEGSTWASAS
                     SGMHSWGSVRAIAVAEETPRSIYVGLSTSGVWRTRDDGKTWSNAVDGIVADPYYGISV
                     RTLEVNPANSEHLLVATEETGGTGSLYSTTNGGNLWSKVTWENTSIVNDLLFVPTQTN
                     IVLAALVGNGIWRSINGGTTWTKIIGLPDVNNATTFTCEAGGRIFCGVIGGFDYPNDY
                     YYSDNAGETWSRGAGAPLVKSFASDPTQPGRVMAGTGWWGSGSGLYFTANNGTEWNAA
                     NTGLPINWGGTYPEADSLIAIPGNAGGYCLAMGNGVYQTQNEGTSWTKITGSEPIQAS
                     VLAYSTRNQGTVFCGSSVNGLWYAQLVEPLPTFTPTPQVSWDLNDDGVIDSLDLLLLS
                     VDWGSNFDTSDLLRLLDTLK"
     gene            complement(5047..>5263)
                     /locus_tag="PKH07_01965"
     CDS             complement(5047..>5263)
                     /locus_tag="PKH07_01965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33742.1"
                     /translation="EDGSVGPEVITQSGNLIKALFERFAHFKTHPVSNGEIRIERADK
                     KRNCLEIYKGEIHPAQHKLSCPACLDA"
BASE COUNT         1215 a         1444 c         1365 g         1239 t
ORIGIN      
        1 ctcgcacgga gaagggttca ccctgtctga cagaaagcat tgatggcacg atggacaatg
       61 caaccgaacc ttggggaatg cgatcccgtt gctgctttct ggcattgcgc agagtagcgt
      121 agggagagag gggctctccg gaaggattga ctcgcactgt tgctgaaagc acaccatcgc
      181 cttcaatttt cggttgccca agaatgtctt cccctttcgc ttccagatat gtccatggaa
      241 gaccgagagg atccaattct gccagactga cattcgcatc tcttcccaca aaacggatgg
      301 tcgtggcaga actgggttca acagcttcaa actggatctg cccgaaactt cccttgaagc
      361 gccgggtgtc tccatccatc atgtgaacat ggtagaaggc cagacctttg tcaggcaaag
      421 atcggttcac catcaaacga tattccgcgt tgggttggat caagacattg ctggcgctct
      481 taactgcagc agaagcctct tcgcccgctg gttcaaccac ggccagaaat ctcggatcgt
      541 aagacagagt gactcccagt ttgtcgaatc gcaattcctt ggggttctcc aaattgaggt
      601 tcagcacaaa acgctgtccc actgagacga tgttggacga cgggcttaaa tagagaatcg
      661 cccgttccga gggcgcggcc tgcacttgtc ccgtagccgt tctcgctcct ggaggagacg
      721 gtggcgcggg agcgtcagga gaaggtccga ctatctccaa accaccggaa cccggctgtc
      781 cggatcctga ctcacgcctg ctgcgaccgt caccccggcg atccgaagat cgtccggagt
      841 ctccggaagt tggttgactc ggtgttgaag tcctttcttc ggagtctctc tctgaactac
      901 gtcgagcgcg ttcctcttca attcgccttc ggtcttggtc cgtgctgctc gttcttctgc
      961 tggtgtcagt gcttctggag ttttccgtcg tcccggtgtt ccgagtcgtc gtgctctcgc
     1021 tgcgttgtct ttcggaacgg taactggtgc tcgaccttcg gttgtctcca aataagtcat
     1081 catcgctcga gcgatctcgg ctgctctgtg ccaaaagcat ggggcgttcc tggatagata
     1141 aggccgatgt ggaatcttcc aatgcctgag cagtggagac cacacattcc gccaccagga
     1201 aacatgcaat gagaagcgtt aaagcaaacc gcatcttgat acctgggtta atctcgtggc
     1261 tttgtgtact catcagaatc atctccattc acatccagaa cagagccaag agtccgtcgc
     1321 aacgccactc acttcccaat cccacgagcc aaactccggc acttggccaa agtttgagat
     1381 attcctttaa ctccgaaagc atatctcaag taaatcctag agtcaagcgt tttgtgcagc
     1441 atagacagtc agctggcaga aagacccttt cttctgttca aatcaagctc acctgagtct
     1501 ttcttgggta ctacctttcc agtgcatatt gtcacaggct ctctcaatag gaaagcctca
     1561 gacatcagaa tatacccaat atcttgtact caggcaactg aaaagaaaca acttgcccat
     1621 attctcgata cacacatcgt caaagcataa tggcataggc ctcctctccc gtccaccctc
     1681 ttttatttct tgacgaatcc ggctatgatt cacttcaata tgagaccaga agggaaatat
     1741 cttcaggagt tttccgactt tttgaccgtg gaacgcgggc tgtcgaaacg cacccgtgaa
     1801 gcctatctga tagacttgga aaatttccgg cagtactgct caggacacgg cctggccatt
     1861 ccaaaagata ttgactgtgt cgaagtcagt cgctatgtcc ttgaccttca ggcatcggga
     1921 agaactcctg ccacggtagg cagggctcag tcgagtctca ggcagttttt tcgctttttg
     1981 gttcatgaga agattaccga tcaggatccc accgtccacc tgcgctcccc aaaacagatc
     2041 aagcacctgc ctcagttctt gactcaggaa gagatggaaa ggctcctggc cgcccccgat
     2101 gcgaccgatc ccagagggat tcgggatcga acgatgctcg aggttctcta tgcgacaggg
     2161 ctgcggattt cagaactcct gtcactcagg atcgaggatg tagatttgcg tttggggttt
     2221 gtcagaaccc gaggcaaggg ggacaaggaa cgaatcatcc ccatgcactc acaggctgtg
     2281 tccctgcttc aggattatat cgagaacacc cgaacagttt tcgacaaggg catcggtgga
     2341 actgttgttt tcctcaatcc ctccggcagg cgtctttctc gaatggggtt ctggaagatt
     2401 ctccgaaaat ggggtctcaa agcaggtatt gccaaatcgt tttccccaca tacactcagg
     2461 cactctttcg cgacacatct gcttcaagga ggcgctgatc tgcgttccat tcaggaactg
     2521 ctgggtcatt cagacatatc aacgactcag acctacaccc atgtcgaaca gaatcggctg
     2581 cgcgaagtgc atcggcgtca ccatcctcgc agttgatctt tggtttccct gtgaaccaga
     2641 ctccatgaaa actcgtctct tcagcaaaaa ggcaacgggt acaagtgggc acagcccgtg
     2701 catgcccggg cactaacctg ctcagaaact ttcctttgac aaagaccaaa ccccttctgg
     2761 catcgttatt accaagaacg aggatgcggt gaggaagtgt gcatctttcc ccacctgatt
     2821 ttgtgatgat tgacttgcag gcatccccct cggatgcaag gagagaagga atgtctttga
     2881 agagatatct gattgtggtc agtgtgtttt gtctctgggc ttcgcttgcc ggttcacttg
     2941 aatggcgtga gttcggtcct ccgggagcaa ccttgctcaa tgtggttcga ggcactggtt
     3001 gggtctatgc ttttggaagg gatcaggtat acaagtcaaa tgacgccggt ttgacttggc
     3061 agaacatgcc ccaagttcct ttggttcagg gcgactatct cgtggacggc attgtcttcg
     3121 gagccactga acaggcgatc ctggcggtga gaagttcaaa aggcatcctt cgatgggatg
     3181 tcgcttcaaa ctcatggatc gaagtcaacc aagggatcac cccccccaca cctccctacg
     3241 gatggcagtt catcgggatc agtgccgcaa ctcccgcatc cacccgtgcc tttgccggtg
     3301 tctccacagg actcttttac agcgacgact ttggcgacag ctggcacaag gtatctgcca
     3361 actatgcttt tcaggggatc gtggcagcgg cggatggttc ggtctactat gactccggat
     3421 cgtcacagca aacccagcct ttccgacttg aaagtgatct gacgactttg actggtttca
     3481 cgatgagacc cggtctgcct cagaatcgag ctatctatgg aacgattgct ccgaaccctg
     3541 agaacccgtt ggagcttctt tttgtcgagt ctgtggacaa caaggcctac tacagcaccg
     3601 atcgaggtga gacctggagc agtccggctt tgggaaccgt cccacaggga gctacaagtg
     3661 cagggtggct caacggaaca cccatcgtct tctcggaatc atctgcctat tccctgaaca
     3721 agcagaccca gacctggagt cttctgtgga ctcgcccagt cgccgaccaa tacaaggtca
     3781 tagctgcgaa cgcgaacacg ctgcttctat gggggcttca gacaaacatc tggcgctcga
     3841 ccaacgaggg ttccacgtgg gcgtcagcat catccggaat gcactcctgg ggaagtgttc
     3901 gagccatcgc cgtcgccgag gaaacacccc gctccatcta tgtcggcctg agcacatcgg
     3961 gagtctggcg aacacgagac gatggcaaga cctggagcaa tgctgttgat ggaattgtcg
     4021 ctgacccata ctacgggatc agcgtgagaa cacttgaagt gaatccagcc aattcagagc
     4081 atcttctggt cgcaactgaa gagacaggcg gcaccggttc gctgtactcg acaacgaatg
     4141 gggggaatct gtggtcgaag gttacctggg agaacacgtc catcgtcaac gatcttctct
     4201 ttgttccaac ccagaccaac atcgtcctcg cggcgttggt tggaaatggc atttggcgga
     4261 gcatcaacgg cgggacaacc tggacgaaaa tcatcggctt gccggatgtg aacaacgcga
     4321 cgacattcac ttgcgaggcc ggtgggcgaa tcttctgcgg cgtcattggc ggcttcgact
     4381 atcccaatga ttactactac agcgacaatg caggtgagac ttggagtcgt ggcgcgggcg
     4441 cgccgctggt aaagtcgttc gcttcagatc cgacccaacc cggaagagtc atggcgggaa
     4501 ccggttggtg gggctcaggc agtggcctgt acttcaccgc aaacaacgga accgaatgga
     4561 acgccgccaa cacagggttg cccatcaact ggggtggcac ctaccctgaa gcggacagcc
     4621 ttattgcgat ccctggcaat gcgggcggtt actgtctggc catggggaat ggcgtctacc
     4681 agactcagaa tgaaggcacc tcctggacga agataaccgg ctccgagccg attcaggcaa
     4741 gcgtacttgc ttattcaacc cgcaaccaag gaaccgtgtt ttgcggctcc tcggtcaacg
     4801 gcctctggta cgcccagttg gtcgaacctc tgccaacgtt caccccaact ccgcaggtca
     4861 gttgggatct caatgacgac ggtgtgatcg actctctcga ccttctgctt ctgtctgtag
     4921 actggggaag caatttcgac actagcgatc tgctccgact gctcgacact ctgaagtgag
     4981 acacggtgaa aagacatata agtctcccag tgactcagag tccaagagag gcgtgtcatc
     5041 taccgatcaa gcatccaagc acgcagggca gctcagttta tgctgcgcgg gatgaatttc
     5101 gcccttgtag atttcgaggc aattgcgttt cttatctgca cgttctattc ttatctcacc
     5161 atttgaaaca gggtgggttt tgaaatgagc aaaacgttcg aacagggcct tgatgagatt
     5221 gccagactgt gtgattactt caggaccaac agagccgtct tct
//
LOCUS       DAOQUC010000068         5251 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_17351_length_5251_cov_3.354888, whole genome shotgun sequence.
ACCESSION   DAOQUC010000068 DAOQUC010000000
VERSION     DAOQUC010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5251
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17351_length_5251_cov_3.354888"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..606
                     /locus_tag="PKH07_01970"
     CDS             <1..606
                     /locus_tag="PKH07_01970"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNT33743.1"
                     /translation="DMLDLSQAEMGRLQPHLQRFEVRHAIDAVLETYAPLFARKELAF
                     EAEISPDLGAMIADPQKLQKVMAILLDNAAKFNEDGGSIRLKVQQCSDIEMKRKGGKH
                     CEGCPGAHCLCVSVQDTGIGIQTEDIEVLFNPFRQLDSSYAREYAGTGIGLALARHLV
                     TLHGGRIWGESEGMGKGSTFTFVIPFEPVTMASRPSNEHER"
     gene            762..3116
                     /locus_tag="PKH07_01975"
     CDS             762..3116
                     /locus_tag="PKH07_01975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33744.1"
                     /translation="MKTVGEQSIGSQDTSSLHGKWLDAFCAAMNVSRLYRQEHPKVVE
                     SLEALLELTRIIQEENEGELALSFAEHTVAINQEEMDPRVLAHRRLAGELHQRNAHTL
                     IFARGINREELSTFMKIFGDHPKNIIKQGGLALLMESAGTHHVRLAALRYASRVDYSE
                     QLKDTEDRILFARVEECLEDEIELKKILEGMDDPSLQLVAFFENPRTTVRFLEHRKGE
                     GESSETFEREVVRTLERAGTIIFTHHPDRWDKVKESIAQALVLLAPIIREKILEIACT
                     MQEESVLLEDVVASLSAEQIAVVLTDYLASRLTRNEADGVLSLNIQTDQERVDGLQSL
                     IEKLIHSPEHFDLLEPLLRDEFQRREMHVESCRTLFEPICGEIFQAAQRTDLAAVSSD
                     KATIFRYATRSDEAHENLSDLLATMNEDSWEASQRTILQELLGREKDPAVYRQILGPR
                     VIRVVKKLQAAGAVKQAREELTLILEHASSPSPEDFLDRAPSARGLLQEMNPTHLVAL
                     YLDDYEQNPDFERDASRLAASQLEGVARELIRRSFAPDQVRTRPPKLLSIVSQNMAFL
                     GSYLGDWLSQNPEHANEDVWLVIRHGLCEGCVPVLELALRSEERDPMVQAVEYLGRIG
                     GNRAESLLLSYSRKGGALGNLAFENLCRLSSPKAVSLIGEQLLKPDYLGIHAKKRRVL
                     VQKLKAIQTQDARRLMAHLMTQNVTNLLLRGRHEKRVLDLMQTLRNWPVEEAYPVVDT
                     LTKHSSRRVASLATGLVKEMPSVDQTKASEATGLLESEERVDQR"
     gene            3113..>3452
                     /locus_tag="PKH07_01980"
     CDS             3113..>3452
                     /locus_tag="PKH07_01980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33745.1"
                     /translation="MRSLKEWHEKSVKIEEGLRYLHLLANDARLFTDNHPKFQDTLRV
                     THEFWSGLLSEMDAIEIKQIGQDLIYEDVPLGTSLKSTQEVSRSFQERGMESLVFTPG
                     LTIQELTEYAH"
     assembly_gap    3453..3462
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3463..>4353
                     /locus_tag="PKH07_01985"
     CDS             <3463..>4353
                     /locus_tag="PKH07_01985"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNT33746.1"
                     /translation="MRLSEQHRSQVGSPSVYFVHRGVTHIQCYRLGDMRDDEARREAE
                     RQAKGLPVAMKDLSAFRCDALKTIQNLYLQAKVANVLDLDTAQGLVDVFLRESIMNST
                     TLLGLCAIRTSDEYTSTHSMNTCLLSLSLARYLGVPDDKLPMVGIACLFHDIGKMFIP
                     DAILNKPGKLTPEEWGVMQSHTVLGARFLLSVPNIPTLAPLVAFEHHMNPEGTGYPCP
                     KRDYKTNLISYAASVADFYDALSTARSYKKAISPEKVVDIMLKLEEGKMEPRLKRQFL
                     RMVGTYPIGSVVRLDTGELA"
     assembly_gap    4354..4363
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4364..4579
                     /locus_tag="PKH07_01990"
     CDS             <4364..4579
                     /locus_tag="PKH07_01990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33747.1"
                     /translation="RQNTEDSHRTRVQLVTAPDGTPLTQSVAAYLTETRDGGIFVRSV
                     SEGVDPTEVQIDALGVLQEILDADRLQ"
     gene            4776..>5251
                     /locus_tag="PKH07_01995"
     CDS             4776..>5251
                     /locus_tag="PKH07_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33748.1"
                     /translation="MPSSSLRRLNRLMQVTAAVVVMWLFLGVSSGSAENITIFSDTMG
                     IVGDRDPDPDMFNGVPFRAVVEDGIAYFYFAGDLIVGTTDTVTLIGSRPISLVVGGDA
                     VFNAGALINASGFGTTPGCGGGAGGAAGEGGSGGAGVWPSPPQYAWGGPGGTGIDY"
BASE COUNT         1217 a         1333 c         1480 g         1201 t
ORIGIN      
        1 gacatgttgg atctctcaca ggcggagatg gggcgtctgc aaccgcacct gcagcgcttt
       61 gaagtgcgcc atgcgattga cgcggtactg gagacctatg ctccgttgtt tgcgcggaaa
      121 gaacttgcat ttgaagcaga gatctctcca gacctcgggg ccatgattgc cgatcctcag
      181 aagttgcaga aggtaatggc aatcctcctg gacaatgcgg cgaaattcaa cgaggacggc
      241 ggttcgatac ggctcaaagt ccagcagtgc tccgatatcg aaatgaagcg aaaaggcggc
      301 aagcactgcg aaggatgccc gggcgcacat tgtctgtgcg tgtccgtgca ggatacggga
      361 attggcattc aaactgagga catcgaagtc cttttcaatc cattcaggca gctggactcc
      421 tcgtacgcca gggaatatgc cggaacaggc atcggactgg cgctggctcg gcatctggtc
      481 actctccacg gtggccggat ttggggcgaa agcgaaggca tgggcaaggg cagcacgttc
      541 acctttgtca tcccgtttga gccggtcacc atggcgagcc gtccgagcaa tgagcacgag
      601 cgatagaccc cgcactgaaa taacacacac gccaaaaaaa agaacactta cacggtcacg
      661 gtgcccctcc tgcagagatc ttgagaaaag tgaggaagat gggtatactg actcttgtag
      721 tattgtgaga atgggactgc tatagacaag gggatacgtt cgtgaagact gttggcgaac
      781 aaagcatcgg atctcaagat acttcctcac tgcatggcaa gtggttggac gctttctgtg
      841 cggcgatgaa cgtctcccgc ctctatcgtc aggagcaccc caaggtcgtt gagtctctcg
      901 aagccctttt ggagttgacc cggatcattc aggaagagaa tgagggggaa ctcgccctct
      961 cgtttgcaga gcatacggtg gccatcaacc aagaggagat ggatccccgc gttctggcgc
     1021 accgtcgttt ggcgggtgaa ctccatcagc gaaacgcaca tacgctgatc ttcgcgcgtg
     1081 gcatcaatcg ggaagaactc agcacgttca tgaagatatt cggggatcac cccaagaaca
     1141 ttatcaagca gggtgggctg gctttgttga tggagagcgc cgggacgcac cacgttcgtc
     1201 ttgcagcgct tcggtatgca tcacgggttg actacagcga gcaactgaag gacacggaag
     1261 accgcattct ctttgcccga gtggaagagt gcctggagga cgagatcgaa ctcaagaaga
     1321 ttcttgaagg gatggatgat ccttcccttc agctggtggc attctttgag aaccccagaa
     1381 cgacagtgcg cttcctcgaa caccgcaaag gggaagggga gtcaagcgag actttcgagc
     1441 gtgaagtagt tcgcactctc gaacgcgcgg ggacgatcat ctttacacat catccggatc
     1501 gatgggacaa ggtcaaagag agtatcgccc aggcgttggt gctgttggcg cccatcattc
     1561 gagagaagat cctcgagatt gcctgcacca tgcaagagga gtccgtgctt cttgaagacg
     1621 ttgttgcctc gctgagtgcg gagcagattg ccgtcgtgct gacggactac cttgcctctc
     1681 gactgactcg aaatgaggcg gacggagtcc tttccttgaa tatacagaca gaccaggaaa
     1741 gggtggacgg tctgcagagc ctgattgaga aactcattca cagtcctgaa cattttgatc
     1801 ttctggaacc tttgcttcgg gacgagtttc aacgacgtga gatgcacgtt gaaagttgtc
     1861 gaacgctttt tgaacctatt tgtggcgaga tattccaggc cgctcagaga acggatctgg
     1921 ctgcggtttc cagcgacaag gcgaccatct ttcggtacgc cacccgaagt gatgaggcac
     1981 atgagaatct ctccgatctg ctggccacga tgaatgaaga ctcatgggaa gcaagccaga
     2041 gaaccattct tcaggaattg cttggcaggg aaaaggatcc tgccgtctac cggcagattc
     2101 tggggccgag ggtgattcgt gtggtaaaga aactccaggc ggcaggggca gtcaagcagg
     2161 cacgagagga acttacgctc attctggaac atgcttcttc cccttctccc gaggatttcc
     2221 tcgacagagc cccctctgcg cggggactct tacaggagat gaatccaact catttggtcg
     2281 ccctctatct tgacgactat gagcagaatc cggattttga acgcgatgca tcgagactcg
     2341 ccgcttctca actggagggg gttgccagag aactcattcg ccgctctttt gccccagatc
     2401 aggtgcgcac ccgaccgcct aaactacttt ccattgtctc gcagaacatg gcatttcttg
     2461 gttcgtatct tggcgactgg ctgagccaga atccggaaca tgcaaacgag gatgtgtggc
     2521 ttgtcattcg ccatgggctg tgcgaaggat gcgttcctgt actggagctt gccttgcgaa
     2581 gcgaagaacg tgaccccatg gtacaagccg tggagtatct tggcaggatt gggggaaacc
     2641 gagcggaatc ccttctgttg tcctattcac ggaagggtgg ggcgttaggg aatcttgcct
     2701 tcgagaatct gtgcaggctt tcttctccca aggcggtgag tctcatcggt gagcaacttc
     2761 tcaagccgga ctacctcggg attcatgcca agaaacggcg tgttctcgta cagaaactga
     2821 aggcgattca gacgcaggac gcccgcagac tcatggcgca tctcatgact cagaatgtga
     2881 cgaatctcct gttgcgaggc agacatgaga agagggtact ggatttgatg caaacgctga
     2941 gaaactggcc tgtcgaggaa gcgtatccgg ttgtggacac actgacaaag cattccagtc
     3001 gaagagtcgc ctccctcgca accggtcttg tgaaagagat gccctcggtg gatcagacaa
     3061 aggcgtctga agccactggc ctgcttgagt cagaagaaag ggttgatcag cggtgagatc
     3121 tctcaaagaa tggcatgaga agagcgtcaa gatagaggaa ggacttcgat accttcacct
     3181 gttggcgaat gatgcgcgtc tgtttaccga caaccacccc aaatttcagg atacgcttcg
     3241 cgttactcat gagttctggt cgggtctgct ttccgaaatg gatgccatcg agatcaagca
     3301 aatcggtcaa gacttgatct acgaagatgt tccactgggc acgtcgctca aatctacaca
     3361 agaagtgagc cggagttttc aagagcgcgg gatggaaagc ctggtgttca cccccggtct
     3421 gaccattcag gagctgacag agtacgctca ttnnnnnnnn nnatatgcgt ctctctgaac
     3481 agcatcgttc gcaagtcggc agcccttcgg tctacttcgt ccatcggggg gtcactcata
     3541 ttcaatgcta ccggcttgga gacatgcgcg acgatgaggc ccgccgcgaa gccgagcgtc
     3601 aggccaaggg actccctgtc gcgatgaagg atctcagtgc atttcgttgc gacgcactga
     3661 aaaccattca gaacctctat cttcaagcga aagtcgcaaa cgttctggat ttggacaccg
     3721 cccaaggact ggtggatgtc tttcttcgag agtccatcat gaattcgacg acgctcttgg
     3781 gactgtgtgc cattcgaact tcggatgagt acacttcaac acattcgatg aacacatgtc
     3841 ttctgtcgct cagtctcgct cggtatctcg gcgttcccga cgacaaactc ccgatggttg
     3901 gcattgcttg tctctttcat gacatcggga agatgttcat ccctgacgca attctgaaca
     3961 agccaggcaa actgactcca gaagagtggg gagtgatgca gagtcacacg gtcttgggtg
     4021 cccgatttct gctttccgtg cccaatatcc cgacgctcgc accccttgtg gcgttcgaac
     4081 accacatgaa tccggaagga acgggatacc cttgccccaa gagggattac aaaaccaacc
     4141 taatcagcta tgccgcgtcg gtcgcagact tctatgatgc tttatctacg gcgcgttctt
     4201 acaagaaagc catatcaccc gagaaggttg tggatatcat gctcaaactc gaagagggca
     4261 agatggaacc acggctcaag agacagtttc tcaggatggt gggaacctat cctatcggct
     4321 cagtggtgag actggacacg ggagaattgg cggnnnnnnn nnnagacaaa atactgagga
     4381 ttcacacaga acccgtgtgc agctggtgac ggcacccgac ggcactccgc tcacacagtc
     4441 cgttgccgca tacctgaccg agacccgtga tggaggcatc tttgttcgtt ccgtttccga
     4501 aggcgttgat ccgacagaag tccagataga tgccctcggt gtgctgcagg aaatcctgga
     4561 tgcagatcgt ttgcagtgag ccggttgccg gaagactgcg ccttgggaac aaacaacctt
     4621 agtacactgc ttttgttctc tcttgtttcc ctttgtcttc gcgatgggaa aatggtaatc
     4681 ttccgtgtgt cagatcggag aatccattcg ttctgattca ctgcattgca cagaaaacag
     4741 tcttcgaagc accaacagga aaggggtttc ttcagatgcc ttcatcatct ttacgacggc
     4801 ttaatcggct catgcaggtg acggctgctg ttgtggtcat gtggttgttc cttggggttt
     4861 catctgggag tgccgagaac atcaccatct tctctgatac tatgggcatt gttggtgacc
     4921 gtgatccgga tccggatatg tttaatggtg ttccattccg cgcggtcgtc gaagacggaa
     4981 ttgcctattt ctattttgcc ggggatctga ttgttggtac aacggacacc gtgaccctta
     5041 tcggatcgcg ccccatctcg ctcgttgtgg gtggtgatgc cgtcttcaat gctggagcat
     5101 tgatcaacgc ctcggggttc ggcacaactc ccggctgtgg gggtggggct ggaggtgccg
     5161 ctggagaagg tggttctggc ggcgctggcg tttggccgtc accacctcaa tatgcctggg
     5221 gtggaccagg cggtacaggt attgattact t
//
LOCUS       DAOQUC010000069         5249 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_17368_length_5249_cov_3.324990, whole genome shotgun sequence.
ACCESSION   DAOQUC010000069 DAOQUC010000000
VERSION     DAOQUC010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5249
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17368_length_5249_cov_3.324990"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="PKH07_02000"
     CDS             <1..190
                     /locus_tag="PKH07_02000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33749.1"
                     /translation="KAKTACMATGIIIVMFLRILESLQLHLPYNAVYLTIFSIITAVT
                     FWSAIDYVWANAKHIRVT"
     gene            187..1353
                     /locus_tag="PKH07_02005"
     CDS             187..1353
                     /locus_tag="PKH07_02005"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNT33750.1"
                     /translation="MKTLLYPRLTAPLLVSLSEQDCRGSEDNQGVNIRCKKTQTDPTP
                     VCSICRPEISLPLHLKSSGSDMSGELSLSTGADSLRETGATPGIYDLKMQIGNCTFVE
                     PHAVRLFNQIPAEYTFCLLTDFHLYPPYTSFPFRLSVAHVRKCLAQAMQLRPEFLLFC
                     GDVSSLIFFYPWSVAAMRRLLIEEVSCPVFFVPGNHDGYLFRLAGLTWWDGWVYWQQH
                     LGPLYQSFDFGQDHFIGLNTFDWQPARRNIFSRAEFLTFRFRLGAFISDEQIRWLERD
                     ISEARKREQRIFFFLHHSPLHSQFFRPNGQTLKGTWEGTNRERVLELICESHAVAVFC
                     GHQHYTAMETWEGIPFFLTASSASYNEPGFDYAFKKITIRNGVLASVETIPFQI"
     gene            complement(1372..3645)
                     /locus_tag="PKH07_02010"
     CDS             complement(1372..3645)
                     /locus_tag="PKH07_02010"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNT33751.1"
                     /translation="MKTSRSVCLLAGLATLLVSALWTGCRTRPIPQSPNVLVVTFDTV
                     RADHFGCYGSPTAHTPNVDALAASGVVFEKCFTPVPITLPAHVSIMTGLYPHEHAIRN
                     NGSYFVPQSVTTLAEVLYDKGYETAAFVSAYVLHSQFGLNQGFQVYDDTVRDSSAVVL
                     IEERTADQTTDQVLRWLGQQRSRPFFLWVHLFDPHQAYMPPAQWANRFPNDPYSGEIA
                     FADEQLGRLLQEVDLSRTLVVFSGDHGEALGEHGEKTHAYFAYDSTLHVPLVIRLAGG
                     VRAGERRSDVVSLVDVFPTVLGHLDFTAPQTPSALDLLSEKKSDAPDRFVYFETIYPL
                     VFGWAPLSGLRNNTHKYIHAPRPELYAVQEDPQELVNRYDEERERAEDLKRRLGNLFV
                     DLQQTDSQRDLSAEDRQKLASLGYVGGALTATDLDLMSLPDPKEALQHYLLYTEAGKE
                     NRAGRYSVAEEILRSLLTVYPDMPQAQMLLGEVLREQARFEEAMSVFERLLRSNPSDN
                     SFWYAQAVTCNKAGLSQKAIEVCTEGLRHFPDEPGLLDQMGLAYKMAEDYKRAIEFGQ
                     RAVRIVPNKPEYLNNLGASYSAAGKYKEAQNFLSRAVSIQPDFSSALFNLGVVALREK
                     HNGQAETYLRQAVAADETNHDAWVQLNKALLYQDKSDEALLISQKLLALNPEDSEPHY
                     FMSVAYRQKLDFSSAEESLRRFLQANPNVGFAYAELCEVLLSHSPPKIQDAREAGEEA
                     QKRGVALPEKLRTQMGR"
     gene            complement(3877..4239)
                     /locus_tag="PKH07_02015"
     CDS             complement(3877..4239)
                     /locus_tag="PKH07_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005009474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNT33752.1"
                     /translation="MARLLVVDDEENIRELYKVELEDDGHQVMTAEDGEDALRLLGEQ
                     TFDLITLDMRMPKMDGVELLGKIRERNLRIPIVVCSAYPGYKQDFHVWGAEAYIVKSS
                     NLTELKETILQLLPGQNK"
     gene            complement(4265..>5249)
                     /locus_tag="PKH07_02020"
     CDS             complement(4265..>5249)
                     /locus_tag="PKH07_02020"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNT33753.1"
                     /translation="SSCTAGLHLLVQALGLGSGDEVIVPSMTFVATVNAVLYVGAKPV
                     FVDIAGVKEPHLDPKSVEAAVTPRTRAIMVMHYGGHPAPMAEYKSVAERHGLALLEDA
                     AHAPGARVKGEAIGSLSAGAAFSFYANKNLSTGEGGMITTNDAVIADLVRSSRSHGMT
                     STSWERHSGRTADYDVERLGYNYRMTEITAALGRVQLRKLPQANQARARLDSQYRERL
                     GELPQIEIPYSADSSGSVHHLFPIVLSSGISRDRFRERLAQQGIQTSIHYRAVHQMSY
                     HKRLRKAGKRVALPITEEYASREVTLPLFPSMTEAQLDAVVQAVRTAVLEA"
BASE COUNT         1165 a         1466 c         1359 g         1259 t
ORIGIN      
        1 caaagcaaag accgcctgca tggccacagg gatcatcatc gtcatgtttc tgcggattct
       61 agagagcctg cagctgcatc ttccgtacaa cgcagtctat ttgaccatct tctcgattat
      121 tacggcggtc actttctggt cggccataga ttatgtctgg gcgaatgcca aacatatccg
      181 ggtaacatga aaactcttct gtatccccga ctgacagcac cgctcctggt ctcgctatca
      241 gagcaggatt gtcgaggctc tgaagacaac cagggggtga atatccggtg caagaagact
      301 cagacagatc caacgcctgt ctgctcgatc tgcaggccgg agatttcgct tccattgcat
      361 ctgaaatcca gcggatcgga catgtccgga gagttgtctc tttcgacggg tgccgattcc
      421 ctgcgagaga caggagccac tcctggcatc tatgatctga agatgcagat cggcaactgc
      481 acttttgtgg agccgcatgc ggtacgtctc ttcaatcaga ttccggcaga gtatacgttc
      541 tgtctgctga cggatttcca cctctaccca ccgtacacct cgtttccttt tagattgagt
      601 gttgcccacg tcaggaagtg tctcgcacag gcgatgcagc tcaggccgga gtttctgctg
      661 ttttgcggtg atgtttcgtc gctcatcttc ttctatccgt ggtctgttgc tgcgatgcgt
      721 cgcctgctga tagaggaagt ctcttgtcca gtcttcttcg tccctggcaa tcatgatgga
      781 tatctctttc ggcttgctgg tctgacgtgg tgggatggtt gggtctactg gcaacaacac
      841 cttggcccct tgtatcaatc cttcgacttc ggccaggatc atttcatcgg actgaatact
      901 ttcgactggc agccagccag aaggaatatc ttcagccgtg ccgagtttct gacctttcga
      961 tttcgacttg gagccttcat cagtgatgag cagatacgct ggcttgaaag ggatatctct
     1021 gaagccagga agcgggagca gcgcatcttc ttctttctcc atcacagccc gctgcattcg
     1081 cagtttttcc gacccaacgg ccaaacgctc aaaggaacat gggaaggcac taacagggaa
     1141 cgagtccttg aactcatctg cgagtcgcac gcagtcgctg tcttctgcgg gcatcaacac
     1201 tacacagcaa tggagacttg ggaaggtatt cccttcttcc taacagcgag ttcggccagc
     1261 tacaatgaac cgggtttcga ctacgcgttc aagaagataa ccatccgcaa cggtgtgctt
     1321 gcctcagtcg agacaattcc cttccaaatc tgaggccgtc ctgtggaacg gttaccttcc
     1381 catttgggtt cggagttttt cgggaagtgc gacgcctcgt ttttgcgctt cttccccagc
     1441 ttcgcgagca tcctgaatct tgggcggtga gtggctcagg agcacttcac acagttcagc
     1501 gtatgcgaac cctacgttcg gattcgcttg gagaaagcgt cgaagtgatt cttccgcgct
     1561 ggaaaaatcc agtttctgcc gataggcaac gctcatgaag taatgaggct ccgagtcttc
     1621 gggattcagt gcgagcagtt tctgcgatat caacaacgcc tcgtccgact tgtcttggta
     1681 gaggagtgcc ttgttcagtt gcacccaggc gtcgtgattg gtttcatcgg cggcaactgc
     1741 ttgacgcaaa taggtttcag cctgaccatt gtgcttttcg cgcaatgcca ccacacccaa
     1801 attgaacagt gctgatgaga agtccggctg aatggacacc gcacgactga ggaaattctg
     1861 agcttcctta tactttcccg cggcggagta acttgcacca agattgttca aatactcggg
     1921 cttgttgggc acgattcgaa cagcacgctg accgaactcg atagccctct tgtagtcttc
     1981 agccatcttg taggccaaac ccatctgatc aagaagcccc ggctcatccg gaaaatgcct
     2041 gagcccttcg gtgcatactt cgatcgcttt ctgactcaaa ccggctttgt tgcaggtgac
     2101 tgcttgggcg taccagaatg aattgtccga gggatttgac ctgagcagac gttcgaaaac
     2161 gctcatcgcc tcttcaaagc gagcctgttc tcgaagcacc tctcccagga gcatttgcgc
     2221 ctgcggcatg tcgggataga cagtcaggag actccgcaga atctcctctg caacggagta
     2281 ccgtcccgcc cgattttcct ttcctgcctc tgtgtagagt agatagtgct ggagtgcctc
     2341 tttgggatca ggcagcgaca tcagatccag gtcggttgca gtaagagcgc ctcccacgta
     2401 gcccagcgat gccagtttct gtcgatcctc ggctgagaga tctcgctgtg aatcggtctg
     2461 ctgaaggtca acgaacagat tgccaagacg cctttttaag tcctctgcgc gttccctttc
     2521 ctcgtcgtac cggttgacca gttcctgggg atcctcctga acagcataga gctcaggtct
     2581 tggggcgtgg atgtacttat gggtgttgtt gcgcagtccg gacaagggtg cccagccaaa
     2641 caccagaggg tagatcgtct caaaatagac aaagcgatcc ggtgcatccg atttcttctc
     2701 actcaataga tcgagtgcgc ttggtgtttg tggtgcagtg aaatccagat gcccgagcac
     2761 tgtgggaaac acatccacga gcgagacgac atccgaacgg cgctctcccg ctcgaacacc
     2821 accggcaagc cgaataacca agggaacatg caacgtgctg tcgtaggcaa aataggcatg
     2881 ggtcttctcg ccgtgttctc ccaaagcctc accatggtca ccggagaaga caacaagcgt
     2941 tcgactcaga tcaacctctt ggagcagccg tcccaactgc tcatcggcaa acgcaatctc
     3001 tccagagtag ggatcgttcg gaaagcgatt tgcccactgt gcaggcggca tataggcctg
     3061 atggggatcg aacagatgca cccagagaaa gaaagggcgc gagcgctgct gtcccaacca
     3121 ccgaagaacc tgatcggtgg tttgatcggc ggtgcgctcc tcaatcagaa ccacggcgga
     3181 tgagtcgcgc accgtgtcat catagacctg aaagccctgg ttgagaccga actgcgaatg
     3241 cagcacatag gcactcacaa acgcggcggt ctcataaccc ttgtcataaa gcacttctgc
     3301 cagtgtggtg acgctctggg gtacaaaata gctgccgttg tttcgaatcg cgtgttcgtg
     3361 tgggtaaagc cctgtcatga tggatacatg agccggcagc gtgatcggaa caggagtgaa
     3421 acatttctca aaaacgacac cggacgctgc cagcgcgtct acgtttggag tgtgcgctgt
     3481 tggacttccg tagcagccaa agtgatcggc tcgtacggta tcaaacgtaa cgaccagcac
     3541 gtttggcgat tggggaatcg gtctggttcg acaaccagtc cagagtgcgc tgaccaaaag
     3601 agtggcaagg cctgccaata gacaaacaga tcgtgaggtt ttcatcttat ccctgttgcc
     3661 ctcagcccaa ccctctgatg agagagggaa ccaaaagtcg gcccgtctgt tgtgatgctc
     3721 caagaagaag tcgagttcaa agcgacaacc tgctcagagt tcaaactccg gtttggtttt
     3781 gtcaaccact tgaaaggagg caacagaaga ggtcgggctc tgaactgaga cttttcccag
     3841 ctttgcctct cttgacacac accgtatcac agaggcttac ttgttctggc cgggaagaag
     3901 ttggagaatg gtttccttca attcagtaag gttggatgat ttgacgatgt atgcctcggc
     3961 tccccagaca tggaaatcct gcttgtaacc tggataggct gagcagacga cgatcgggat
     4021 cctcagattc ctttcacgaa tcttcccaag cagttccaca ccatccatct tgggcatgcg
     4081 catatcaagc gtgatgagat caaaagtttg ttcaccaagg agtcgtagcg cgtcttctcc
     4141 atcctccgca gtcatgacct gatgtccgtc atcttccagc tccaccttat agagctcgcg
     4201 gatgttttcc tcgtcgtcca cgacaagcaa tcgtgccatg acccaaatcc ctcctctacc
     4261 gccttcatgc ctccaagacc gccgttcgca cagcctgcac tacagcatct agctgcgctt
     4321 ccgtcatgga cggaaacagc ggcaaggtca cttcacggga agcatactct tctgtgattg
     4381 gaagggcaac ccgttttcct gctttgcgca gccttttgtg gtaactcatt tgatggactg
     4441 cccgatagtg gatgcttgtc tgaatcccct gctgtgccag gcgttcacga aacctgtctc
     4501 gtgaaatccc tgaactcagg acaatcggaa acaagtgatg caccgacccg gaactgtccg
     4561 cactgtaggg aatctcaatc tgtggtagtt caccaagcct ctcgcggtat tgactgtcca
     4621 gtcgggctcg ggcctggttg gcttgcggga gttttcttag ctggactctt cccaacgctg
     4681 cggtgatctc ggtcatgcgg tagttgtatc ccagcctctc cacgtcgtag tctgccgtcc
     4741 ggcctgaatg gcgctcccaa ctcgttgacg tcatcccatg agaacgggaa gaacgcacca
     4801 gatcggcaat tactgcgtcg ttggtggtga tcattccccc ttcacctgtg gacaggttct
     4861 tgttggcata gaagctgaaa gccgctccgg cgctcagaga accgatcgcc tcacccttca
     4921 ctcgggcacc tggcgcatgt gctgcatcct caagcaacgc caacccatga cgctcggcaa
     4981 cgctcttgta ttccgccatc ggagcgggat gtcccccgta atgcatgacc atgatcgcgc
     5041 gggttcgagg ggtgactgcc gcctcaacag atttgggatc cagatggggt tccttcactc
     5101 ctgcaatatc cacaaaaacg ggcttggctc ccacgtaaag caccgcattc actgttgcaa
     5161 caaaagtcat ggatggtacg atcacttcat cgcccgaacc cagtccgagc gcctgtacca
     5221 gcagatgaag ccccgccgtg caggaactg
//
LOCUS       DAOQUC010000070         5229 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_17463_length_5229_cov_3.292617, whole genome shotgun sequence.
ACCESSION   DAOQUC010000070 DAOQUC010000000
VERSION     DAOQUC010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5229
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17463_length_5229_cov_3.292617"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="PKH07_02025"
     CDS             complement(<1..218)
                     /locus_tag="PKH07_02025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33754.1"
                     /translation="MVSPVPLSSQNRIRLGSLESLVVLTRMLPLSELRFAEDLVGRNG
                     TVLIRSGQSLTPSWLEYLKNHDSYVPHFV"
     gene            827..2134
                     /locus_tag="PKH07_02030"
     CDS             827..2134
                     /locus_tag="PKH07_02030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33755.1"
                     /translation="MNTIQRVLLVLLTAFIAMPAFAVICVDYQYVGGQYSNRDTNLDL
                     QVSSAASDIVNGVTPTFVSGGFHSATVPGTRAERLATLVDGAWAPNGLTVIAADYGQL
                     VIEWPFGTPQTLYSIVVFAGHDGDGARAFINCKAEVDTGSGYTTLIDEAKTGDYGLSH
                     QSAASSYIRIRDTNKNALVTGCTKLRLTFYDTAHAGGSLFTAPSDGSTVQGTILKEID
                     VFNTPQTDNITPIPTYTPTNTPEPITITHGGQYAARLQWTNTWASGMDCVLDRFLTRV
                     PVTGGNEIKFGAWVRSATQGMSPMFSMRYAQFQGATWTGDKDQWFTAHDGWQYYSHNL
                     ITNATTTTINLAFRLVTTGGGAIVLDDVTLDDVTAGTPIELTNNGFEDWPGEETSAPA
                     DWRFSLVGGAVGEVRRLTQPTPTNTPEPTNTPSSNVREFEVYE"
     gene            2287..2916
                     /gene="lexA"
                     /locus_tag="PKH07_02035"
     CDS             2287..2916
                     /gene="lexA"
                     /locus_tag="PKH07_02035"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020523519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HNT33756.1"
                     /translation="MSPVSERQQAILNFIHQFHAEHGFPPSVREIGVHFGIKSPNGVA
                     YHLQVLEREGLLEREPRTSRSLRPHGSATQWKPCKVPVVGRVAAGGPNLAFEEIEREV
                     YLDPDLFGNHEGSDSLFALRVTGDSMNGAGIFEGDIAVVRRQHTANLGDIVVAMVEGE
                     ATIKRYLPRNGQVVLQPENDRYSPIVVKGYHLEQLSILGKVIGLLRTRV"
     gene            2924..3172
                     /locus_tag="PKH07_02040"
     CDS             2924..3172
                     /locus_tag="PKH07_02040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33757.1"
                     /translation="MNQALHEPIEVLTKFQHGQIVPLRIRWNDRVYNVEKITGNWFHR
                     DGDFKEYFFAALVTGNRLVEIHLDTRDMRWTLDRIDEE"
     gene            3176..4345
                     /gene="dinB"
                     /locus_tag="PKH07_02045"
     CDS             3176..4345
                     /gene="dinB"
                     /locus_tag="PKH07_02045"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF002677.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HNT33758.1"
                     /translation="MRTIFHVDMDAFFASVEVLCNPKLRGKPVIVSGSPETRSVVSST
                     TYETRKFGIRSGMSAVEARMRCPQAIFVEGNPKKYLYYSMRIQEILFRFSPQMEPFSI
                     DEAFLEVAPCWHQAGRVESLAFQIKQTIKEELFLTASVGAAANKMMAKIGSKMNKPDG
                     VTVIRPEQTQKVLWSLPVEKIWGVGEKTSKALNDLGVVTVQDLAQIPVEWLIRVFGKN
                     GEALRWLAQGIDPTPVKYFDEEEPPKSLGHDITLEKDSGDEQVLLQTLCYLADKVASR
                     LRREQYKAGQLTLKIKYSNFRLRTSQMPLLNYSNYDTDFFGIAQTLWKKMLPLERGVR
                     MLGLSASKLLPIEIANAQQTFFDAEEKKVVGLCQAIDRIRHRFGQTAIARGRCIE"
     gene            complement(4403..>5229)
                     /locus_tag="PKH07_02050"
     CDS             complement(4403..>5229)
                     /locus_tag="PKH07_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011551789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008612 - peptidyl-lysine modification to
                     peptidyl-hypusine [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HNT33759.1"
                     /translation="TGANLEESLFRLVAHKSYKDFPNYRYFTKEDDTLILDQGMRRVT
                     DTSIPEDEAFRAVEKIIVPIWKSATEHGQSRFWHEYLYEVVLSLSSNMYEGDPDECWL
                     LAAAQNNLPIVVPGHEDSTFGNIFASYVKTGECRASIVKSGIEYMVAFYDQYRELSQG
                     AGVGFFQIGGGIAGDFPICVVPSIKYDLREPVRPWAYFCQISDSTTSYGSYSGATPNE
                     KITWDKLTKATPMFVIESDATIVTPLILTSLLECRRDPVRAKELFGEFASEIQYER"
BASE COUNT         1353 a         1267 c         1356 g         1253 t
ORIGIN      
        1 acaaaatgtg gaacgtaact gtcatggttc ttgagatatt ccaaccagga gggggtgaga
       61 ctctgcccac ttcggattag gacagtcccg tttcgaccca ccaaatcctc agcaaatcgg
      121 agttccgata ggggaagcat ccttgtcagg acgaccaagg actccaagga acccaatctg
      181 attctgttct gcgaactcaa aggaactgga ctgaccatcg caatgctccc tcgatagcgc
      241 aaagataaaa caacgcaaaa caacaaagtg aaatcgcctg aggggtcagg gttgttgact
      301 ctgaaactgc ccaacgcgat tcacgataga gcctctggtc acacaagcgt gacggataca
      361 aaacaactta tcgcatttct gtctgaagct tacttcggga cataccctct gtcaacaata
      421 aagcaacaaa aaaaatcgtt tatctggaat cggcaccacg tcaagagtgt gcagatcaaa
      481 tggggcttgg ttccacaggg atccgttcat ccggacagaa cccgagttgt ctggaatgcg
      541 tatgatccga ctctataatg tcattggtga gggaagaaac gtgcggtcgg tttcgtccat
      601 gcagaaggct cccgattcga gttttgtgaa gaaaaggtta atcgtttcag taaatcacgt
      661 tttgtgcttg acagaaggca cggtttgctg aaccattgct ttagttttcg acgggaataa
      721 tggatggagg tgatttgttc gatcatatgg atcatttgaa gtttgatgca aggtcttgag
      781 ggacatttca tgtccctcgg aaaaaaatgc aaggagctac aagaagatga atacgattca
      841 aagagttctt ttggtcttgc tgacagcgtt tatcgcgatg ccggcttttg cagtgatttg
      901 tgtggactat cagtatgttg gtggtcagta cagcaatcga gacacgaacc tggatctgca
      961 ggtttcctct gccgccagtg acattgtgaa cggagttact cctactttcg tatcaggcgg
     1021 tttccattcc gcaactgtcc caggaacgcg tgccgagcgt ttggcaactt tggttgatgg
     1081 agcctgggca cccaatgggc tcacggtcat cgcggcagac tacggacagt tggtcatcga
     1141 atggccgttt ggcactcccc aaacgctcta ttctatcgtc gtttttgcag gtcacgacgg
     1201 agacggcgca cgcgccttta tcaactgcaa agcggaagtg gataccggtt caggctacac
     1261 cacgctgatt gatgaagcca aaactggaga ctatggtctg tcgcatcagt cggctgcatc
     1321 gtcttacatc cgcattcgtg atacgaacaa gaacgctctc gtgacaggtt gcaccaaact
     1381 gcgtctcacg ttctatgaca ccgcccatgc cggtggttcc ctattcactg ctccctcaga
     1441 tgggtcaacg gttcagggaa ctatcctgaa ggaaatagac gttttcaata ctccccaaac
     1501 tgacaatatc actccgatcc ccacatacac gccaaccaac acaccggaac ctattaccat
     1561 cacgcatggc ggacagtacg cggcacgact tcagtggaca aatacttggg caagcggcat
     1621 ggactgcgtt ctggaccgat tcctgacccg tgtccccgtt accggtggca atgagatcaa
     1681 attcggcgca tgggtacgaa gcgccacaca gggaatgagc ccgatgttca gtatgcgcta
     1741 tgcccagttc caaggggcta cctggactgg agacaaagat caatggttca cagctcatga
     1801 cggttggcag tattactctc acaatctgat taccaatgcg acaacaacga cgatcaattt
     1861 ggcattccga ttggtcacaa cgggtggcgg tgccatcgtc cttgatgatg tcacgctgga
     1921 tgacgtaaca gcaggaacac ccattgaact gaccaacaac ggctttgagg actggccggg
     1981 tgaggaaacc agcgcacctg cggactggcg attttctctc gtaggtggag cagtcggtga
     2041 agttcgacga ttgacccaac ccacaccgac aaacaccccc gaaccgacga acaccccgtc
     2101 gtcgaacgtt cgcgaattcg aagtttacga ataaacggac acagggagat gagatgacaa
     2161 tccggctccg gtggcatctg ctgccggagc cggtgccatt tcagagggct ttctttttct
     2221 cttgacaact gtacgtctgt atagtagatg tacttttgta cagtggatag aaaaggagtt
     2281 gacgccatga gtccggtatc agaaagacag caagcgattt tgaattttat tcatcagttt
     2341 cacgctgagc atggatttcc accttccgtg cgggaaatcg gggttcattt tggcatcaaa
     2401 tctcccaatg gtgtggccta tcatcttcag gttttggagc gggagggact cttggagagg
     2461 gaaccgagga cgtcccggag tttgcgtcct cacggttctg ccacacagtg gaaaccgtgc
     2521 aaggtgcccg tggtgggtcg tgttgctgca gggggtccta atcttgcctt tgaggaaatc
     2581 gaaagagaag tttatctaga tccggatctt ttcgggaatc atgagggttc agattctctt
     2641 ttcgcgcttc gtgtcactgg agacagtatg aatggcgcag ggatttttga gggggatatt
     2701 gcggtggtgc ggcgacagca tacggcgaat ctcggcgaca ttgtcgtggc gatggtggaa
     2761 ggtgaagcaa ccattaaacg ctacctgccc cgtaatggtc aggttgtgtt gcagccggaa
     2821 aatgatcgtt attcccccat tgtcgtgaaa ggctatcatc tggaacaact ctccattttg
     2881 ggaaaagtga tcgggctgct ccgcaccaga gtctgaaagc atcatgaatc aagcattgca
     2941 tgaaccaata gaagttttga cgaaattcca gcatggacaa attgttccct tgcggattcg
     3001 atggaatgat cgggtttaca acgtcgaaaa aatcacgggc aattggttcc atcgggatgg
     3061 tgatttcaag gagtattttt tcgctgccct ggtgacgggc aatcggctgg tggaaattca
     3121 tctggacaca cgggatatgc ggtggacgct ggatcggatt gatgaggaat gacccatgcg
     3181 aacgattttc cacgtggata tggacgcttt ttttgcgtcg gtggaagtct tgtgcaatcc
     3241 gaaattgcgt ggaaaacccg ttattgtcag cgggagcccc gaaacgcgtt ccgtggtcag
     3301 ttccaccacc tatgaaacca ggaaattcgg gattcgttcc ggaatgtccg cagtcgaagc
     3361 ccgaatgcgt tgtccgcagg caatttttgt cgaaggcaat cccaaaaagt atctctacta
     3421 ttccatgaga attcaggaga ttctttttcg cttttcccct cagatggaac cgttcagcat
     3481 tgatgaagcc tttttggaag tggctccctg ttggcaccag gcgggtcggg ttgagtcgct
     3541 tgctttccag atcaagcaga ccattaagga agaactgttt ttgactgctt cggtcggagc
     3601 agctgccaac aagatgatgg caaaaatcgg ctccaagatg aacaagcccg atggggtgac
     3661 ggtcattcgt cctgaacaaa cacaaaaagt actctggtca cttccagtgg aaaaaatatg
     3721 gggcgtgggt gaaaagacaa gcaaggcgct gaatgatttg ggagtcgtca ccgttcaaga
     3781 tctggcgcag attcctgtgg aatggctcat tcgggttttt ggaaaaaacg gggaagcact
     3841 tcgttggctt gctcaaggca ttgatccaac gccggtgaag tattttgatg aagaggaacc
     3901 acccaaatct cttgggcatg atatcacgct tgagaaagat tccggagacg aacaggttct
     3961 gcttcaaacg ttgtgttatc tcgcagacaa ggtggcatcc cgcctgagac gcgaacagta
     4021 caaagcggga cagttgacct tgaaaatcaa atactccaat tttcggttgc gcacgagcca
     4081 gatgcccttg ctgaactaca gcaattacga tacggacttt ttcggtatcg cccaaacact
     4141 ctggaagaaa atgctcccct tagaacgtgg cgtgcgaatg ctcggtttaa gcgcatccaa
     4201 attgcttccc atcgaaatcg ccaacgccca acaaaccttc ttcgatgctg aggagaaaaa
     4261 agtggtcggt ttatgccaag caatagaccg aattcggcac cgcttcggac aaaccgccat
     4321 tgcccgagga cgctgtatcg agtgattggt gcaagacctg tcgtgtggaa actatcccac
     4381 gcacacaatc accgaatcga aattaccgct cgtactgaat ctctgatgca aattccccga
     4441 acaattcctt ggcacgaacg ggatctcgtc ggcattcgag cagtgacgtg agaatgagtg
     4501 gcgtaacgat agttgcatcc gactcaatga caaacatggg agttgccttg gtcagtttgt
     4561 cccatgtgat cttctcattc ggtgtcgctc ccgagtagga accatacgaa gtggtcgaat
     4621 cagagatctg acagaaataa gcccatgggc gcacgggttc tcgaagatca tatttgatgg
     4681 atggaacgac gcaaatgggg aaatcaccgg caatgcctcc accaatctga aagaagccca
     4741 caccggcacc ctgtgacagt tcccgatact gatcgtaaaa ggcaaccatg tattcaatgc
     4801 ctgacttcac aatggatgcg cggcattcac cggttttcac ataagacgca aagatatttc
     4861 caaatgtcga atcctcatgg ccagggacaa cgatgggaag gttgttctgc gccgcggcga
     4921 gaagccagca ttcatcagga tctccctcat acatgttcga actgagactg agaacgactt
     4981 cgtagagata ctcatgccag aaccgagact gcccatgctc tgtcgccgat ttccagatgg
     5041 gaacgataat cttctctaca gcgcgaaagg cctcatcttc cggaatgctg gtatcagtga
     5101 cccgacgcat cccctgatcc agaatcagag tgtcatcctc tttggtgaaa tagcgatagt
     5161 ttggaaagtc cttgtaactc ttgtgtgcca ccaaacgaaa cagtgactct tccaggttgg
     5221 caccagtca
//
LOCUS       DAOQUC010000071         5219 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_17504_length_5219_cov_3.195004, whole genome shotgun sequence.
ACCESSION   DAOQUC010000071 DAOQUC010000000
VERSION     DAOQUC010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5219
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17504_length_5219_cov_3.195004"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="PKH07_02055"
     CDS             complement(<1..209)
                     /locus_tag="PKH07_02055"
                     /inference="COORDINATES: protein motif:HMM:NF014615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HNT33760.1"
                     /translation="MDIQEARAALLAVKELGDYFTIVTMTYEKEGRTLNGTDPVTALI
                     TLQSLGADAVGCNCSTGPDGMMDWIV"
     gene            complement(283..801)
                     /locus_tag="PKH07_02060"
                     /pseudo
     CDS             complement(283..801)
                     /locus_tag="PKH07_02060"
                     /inference="COORDINATES: protein motif:HMM:NF024909.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
     gene            1080..2243
                     /locus_tag="PKH07_02065"
     CDS             1080..2243
                     /locus_tag="PKH07_02065"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNT33761.1"
                     /translation="MILVAAVIMQMCLGATYAWNVFVAPLKEISGLDQGPVQLPLSIF
                     YVAFPLTVLFSPWLVRNLGPRRCAIIGGVLFGGGWLTASLGQSHFGIVVVSIGVLAGI
                     GVGFAYLVPISTCILWFPNNKGLVTGIAVAGFGGGAALVSRISGYLIQDLAFDPFHTF
                     RVLGTCFILAIGVAGMFMRLPPGCTANRQRPFPLSDFLNARPFQILYFGMFAGLVAGL
                     GINGNLKQMCRVEHFASSASVAPVALFAIANAAGRITWGALFDRTSPSLILRLNLLSQ
                     ATVLLLAHWILGNASGLTVFALLTGFNYGGVLVLYASSVAHIWGPQRVGQVYSWVFSA
                     NIFASQAPFVMGLGYDAMGSFTVPLLVVSALLLTAFVLASQIRNTLTKVSQHQ"
     gene            complement(2293..3294)
                     /locus_tag="PKH07_02070"
     CDS             complement(2293..3294)
                     /locus_tag="PKH07_02070"
                     /inference="COORDINATES: protein motif:HMM:NF013986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HNT33762.1"
                     /translation="MSKYIIGVDGGGTKTSAAIADLEGNVLTTRTAPSSNYHLVGLEK
                     TEQVFQTLIEQLLLTQAASREDLAAVCFGLSGVGRPDDMARMEAIMVKLGILQKTVIV
                     NDAVIALMGGALTDHGVIVISGTGSIIFGMDKQGSVDRVGGWGHVLDDEGSGYKIGSD
                     GLRLIMRAHDGRHQPTKVTQLVLRALNLEKPESLVVWSAQEGVKKPVVAGLAAHVFEA
                     YRLGDPGAEEIVKHHSAELALGVEIVVKRMQLPATTNVVLCGGVFSANPEYFMLTRTR
                     ILASAEIADVIGPKMPPVVGALLCAFRRKIGVEITRGMLHNMRETFFSVSNEGSSSD"
     gene            complement(3428..3790)
                     /locus_tag="PKH07_02075"
     CDS             complement(3428..3790)
                     /locus_tag="PKH07_02075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33763.1"
                     /translation="MASTRKTKNSASKTKRIVGKRKKNEPHFSFSWKVFALLGTIVGV
                     MIVLLLLSNWQSGKTKVRGDIVKLQEEYQKEVNRQTYEHERQMSKLDEQSDTLQTEID
                     ELKLKIEIARGEKKVLGQ"
     gene            complement(3876..>5219)
                     /locus_tag="PKH07_02080"
     CDS             complement(3876..>5219)
                     /locus_tag="PKH07_02080"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HNT33764.1"
                     /translation="RFENYYASDAPCLPSRSALFSGQFGIHTGVVNHGGTAADPFIQG
                     KSRGFKTDAYHTSWMSLLRKAGFHTVSVSPFAERHSAWWFCSGFNEMINTGRSGLERA
                     HEITPAALDWISRNGEKDNWFLQINFWDPHTPYTTPTEYGNPFANDPPPSWHTEEILK
                     KNWDGYGPHSAREPISWGGEKAPHPRVPTEIRTMEDYRRWIDGYDLGIHYMDSHIGRI
                     LDLLNEKRVLDDTVIIVSSDHGENQGELNIYGDHHTADHITSRVPLIIRWPGMKSNIV
                     DKGLHYNLDLPPTLAEMLKVPAPEVWDGQSFAETITSGQSAGREFLVVSQCAWSCQRS
                     VRFGPWIMIRSYHDGLKDFPPIMLFNVDEDPHETCNLAESYPEVVNKALALLHEWTAD
                     MMKSSSQEVDPLWTVIREGGPLHTRGYLKSYCERLRATGRAHHAEALLSRHRENN"
BASE COUNT         1179 a         1485 c         1256 g         1299 t
ORIGIN      
        1 acgatccagt ccatcatgcc gtccggaccg gtcgagcagt tgcagccgac ggcatcggcg
       61 ccgagactct ggagggtgat gagggcggtg accggatcgg tcccgttgag ggtccgcccc
      121 tccttttcgt aggtcatcgt cacaatcgta aaataatcgc ccaattcctt tacggccagc
      181 aaggccgccc gggcctcctg aatatccatc atggtttcga tgacgaagca gtcgaagccc
      241 ttcgagtacc aagtccgcgt cctggagaag atccgaaacg atgacaataa gaccccgccg
      301 gctcacacgc tctgctacac ttgtcagatt ctcggcaatc cgcgtttgag aatgcccatc
      361 cagtcgcgtc aaagcatcaa ggatcgcctg caggtgtccc agatggccac gaggcggaac
      421 atgctcaaca atctgctcgg ataccccaaa gaaaccagtc gcatcccgtt gcctcaagag
      481 aagaaaactc agagacgcga tcaatatctt ggcatactca agtttggtca ctccccgttc
      541 gggaaactgc atcgaagcgc tcgtgtccag aacgaaatag gccttgaggt tcgtttcctc
      601 ttcaaactgt ttcaggtgca gtttgtcgga ctttgcggca gccttccagt caatgtgacg
      661 cagatcgtct ccgggaaggt attggcgatg ctcagcgaac tcaacgttga atccatgata
      721 ggggctttta tgcaggcccg agatgaatcc ctcaacaacc aaccgcgatg atatctccaa
      781 gtccccaaga ctgctcagca ccacgggatc aaaatagttt ccctgagaag gggcttgagt
      841 ctgtatgttt cctggctgct ctctcaaaac gacatctcct ctgttactca gtgaatatag
      901 cccagccgcc ctgcaatctc aatgtgagag gaaaattgga caaatgatcc gaggttgaat
      961 atgatcttcg gaattcacgc ctctgtggca actacttgga gaagacaacc gtaatgccgg
     1021 acatctttcg ttcaaaccct ttcctgagtt tcacccaccg agttttggtg tcgcctatca
     1081 tgattctcgt cgcggccgta atcatgcaaa tgtgtctggg agctacctat gcatggaatg
     1141 ttttcgtcgc gccattgaaa gagatttcag gtctcgatca agggccagtc caacttccgt
     1201 tgagcatctt ctatgtggcg ttccccctca cagtgctctt ctctccctgg cttgtcagga
     1261 atctggggcc tcgccgatgc gccatcatcg ggggtgttct ctttggtggg gggtggctca
     1321 ctgcaagtct tggacagtcg catttcggga tcgtggtcgt gtcaattggg gtgctcgctg
     1381 gaatcggagt cggtttcgcc tatctcgttc cgatttccac atgcatcctg tggttcccca
     1441 acaacaaggg acttgtgact ggaatagcag tggcagggtt tggaggagga gcggctcttg
     1501 taagccgcat ttcaggctac ctcatccaag acttggcttt cgatcccttc catactttcc
     1561 gtgtgcttgg tacgtgcttc atactcgcaa ttggggttgc cggtatgttc atgcgactgc
     1621 caccaggttg cacggcaaac aggcaaaggc cttttcctct gagcgacttt ctgaatgcca
     1681 gaccctttca gatcctttat ttcggaatgt tcgctggatt ggttgccggt cttggcatca
     1741 atggaaatct gaaacagatg tgtcgtgttg aacactttgc ttccagtgcc tcggtggctc
     1801 cggtggcact ctttgcgata gccaacgcgg caggaaggat cacctggggg gcactttttg
     1861 acaggacttc tccatccttg atcctccgac tcaatctctt gagccaggcc accgttctgc
     1921 tgctggcaca ctggattctg ggaaacgcat caggactgac agtcttcgca ctcctgaccg
     1981 gatttaacta tggcggtgtc ctcgtccttt acgcttcgtc agtggcacat atttgggggc
     2041 ctcaacgagt tggacaggtc tacagctggg tcttctcggc aaacatcttc gcatcacaag
     2101 ctccgtttgt tatgggtctc ggatacgacg caatgggcag tttcaccgtt ccactgctgg
     2161 ttgtgtcagc gctgcttctg acagcgtttg tcttggcaag ccaaatcagg aacaccttga
     2221 caaaagtgtc acaacatcag tgagtaaaga caagacctgg ttcttcgttg gacagatttc
     2281 acccgaagga gatcagtcgg aagaactacc ttcattgctc acagagaaga atgtctctct
     2341 catgttatga agcatcccgc gtgtgatctc aacgccaatt ttcctgcgaa atgcgcaaag
     2401 caaggcaccc acaaccggtg gcattttcgg cccgatcacg tccgcgatct cggcagaagc
     2461 aagaattcga gtccgagtca gcatgaagta ttcgggattg gcactgaaga ccccaccaca
     2521 aaggacaaca tttgttgtgg cgggaagctg catcctcttg acgacaatct caacacccag
     2581 agccagttcc gctgaatggt gcttgacaat ctcctcggca cccggatctc ccagacggta
     2641 ggcctcaaag acgtgagccg cgagtcctgc aacaaccggt ttcttgacgc cttcctgcgc
     2701 agaccagacg accagcgact caggcttctc gagattcaga gcacgcaaga ccaactgagt
     2761 gactttggtc ggttgatgtc tgccatcatg cgcccgcatg ataagcctca ggccatcact
     2821 tccaatcttg tacccactcc cctcatcatc caacacatgt ccccatccac caactcgatc
     2881 gacacttcct tgtttgtcca ttccgaagat gatggaacca gttcctgaga tgacaatgac
     2941 accatgatcc gtcaacgccc cacccatgag agcgatgaca gcatcattga caataacggt
     3001 cttctgcagg atgcccagtt tgaccatgat ggcttccata cgcgccatat catccggcct
     3061 tccgacaccg gaaagaccaa aacaaacggc agcaagatcc tcacgagatg ccgcctgagt
     3121 taacagcagt tgctcgatga gagtctgaaa tacctgctcc gttttctcca accctacgag
     3181 atggtagttc gaagagggag ccgtccgagt tgtcagaacg tttccttcaa gatccgcaat
     3241 ggcagcactt gtcttagtgc caccgccatc cacgccgatg atgtacttgc tcacaatacc
     3301 tctcctcgtc tgtatatcgc ccgctaagcc ttcctaggtc accagaatag cacgttgagg
     3361 aaagcaggcc gtgtcactca actactggcg atcccgatag accgccctat ctgagaatac
     3421 cgaaacgcta ctgccccaaa accttcttct caccccttgc gatttcaatc ttcaatttca
     3481 gttcatcaat ctcagtctgt agggtgtcgg actgttcatc caatttgctc atctgccgtt
     3541 cgtgctcata ggtctgacga ttgacttcct tttgatactc ctcctggagt ttcacgatgt
     3601 caccacgaac tttggtttta cctgactgcc agttgctcaa gagcaagagc acgatcatca
     3661 caccaacgat cgttcccaga agtgcaaaca ctttccacga gaacgaaaaa tgcggctcat
     3721 tcttctttcg ttttccaacg attctcttgg tctttgaggc gctgtttttt gtctttcgag
     3781 tcgaagccat aagccctctt ccgcaatgcc aagtctctaa aatctaggca ttctatacca
     3841 ttgtcatgcc ggagtgtcca gctcgccgct tgaactcagt tgttctccct atgccgtgac
     3901 agaagcgctt ccgcgtgatg tgcccgaccc gtcgcgcgga gcctctcaca atagctcttg
     3961 agataacccc ttgtgtgcag aggtcctccc tcacggatca ccgtccacag tggatccacc
     4021 tcttggctgg agctcttcat catatcggcc gtccactcat gaagcaatgc cagagccttg
     4081 ttcactactt cagggtaaga ctccgccaag ttgcacgtct cgtgcggatc ctcatccacg
     4141 ttgaacagca tgatcggcgg gaagtccttc aaaccgtcat ggtaactccg tatcataatc
     4201 cacgggccaa aacgtaccga cctctgacaa ctccatgcac attggctgac aacaagaaac
     4261 tcccgccccg cagactgtcc gctcgttatc gtttccgcaa aactctgtcc atcccagact
     4321 tccggtgccg gaaccttgag catctcggcc aaggtcggag gaagatcaag attgtagtgc
     4381 aaccccttgt ctacaatgtt gctcttcatg cctggccagc ggataatcaa gggaacgcgt
     4441 gaagtgatat ggtctgcagt gtgatgatct ccatagatgt tcagctcgcc ctggttctca
     4501 ccgtgatcag aactgacgat gatgaccgta tcatccaaga cccttttctc attcagaaga
     4561 tcaaggattc tgccaatgtg gctgtccatg tagtgaatcc ctagatcgta tccatcaatc
     4621 cagcggcgat agtcttccat tgttcggatc tctgtgggca ctctcggatg cggtgctttc
     4681 tcaccacccc atgaaatcgg ttcccgagca ctgtgcggtc catacccatc ccagttcttc
     4741 ttgagaattt cctcagtatg ccaggagggt gggggatcgt ttgcgaatgg gtttccgtac
     4801 tcggttggcg tggtataggg agtatgcgga tcccagaagt tgatctgaag aaaccagttg
     4861 tctttctccc cgtttcttga aatccaatcc aaagcagccg gggtaatctc atgagctctt
     4921 tcgagaccgc ttcgccctgt gttgatcatc tcattgaacc cagagcagaa ccaccaggcg
     4981 gaatggcgct ctgcaaatgg actcacggag acagtgtgaa atcccgcttt ccgcagcagg
     5041 ctcatccaac tcgtatgata ggcatctgtc ttgaatcctc ggcttttgcc ctggatgaat
     5101 gggtcggcgg ctgttccgcc atggtttacc actccggtat ggatgccgaa ctgacctgag
     5161 aacaacgcag agcgagatgg gaggcagggc gcatcactgg cgtaatagtt ctcaaagcg
//
LOCUS       DAOQUC010000072         5218 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_17510_length_5218_cov_3.472012, whole genome shotgun sequence.
ACCESSION   DAOQUC010000072 DAOQUC010000000
VERSION     DAOQUC010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5218
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17510_length_5218_cov_3.472012"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..648
                     /locus_tag="PKH07_02085"
     CDS             <1..648
                     /locus_tag="PKH07_02085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33765.1"
                     /translation="PLGLLNLFLAGAVPIGLLIFTHVESRSYVPPRYALYPVFLMTLM
                     VAGLFLNIRHVSRVMGWLLLAAFLATNCVSVYQRQAHPVIRTTMVAETDQVILDFLKE
                     RGISAACTSLDSPDSGYWNAYRLSASSGDTIFIHPILHMPRIERYRAALERAEHCAIL
                     TPDPERTEVVFQRNNINYSRFDHGGMTVLCDFDKTHVDRLKLIDYRRSLREERTR"
     gene            755..2491
                     /gene="polX"
                     /locus_tag="PKH07_02090"
     CDS             755..2491
                     /gene="polX"
                     /locus_tag="PKH07_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003868128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HNT33766.1"
                     /translation="MDRESVIAILDEIAILLELKGENPFKSRAYSNAARALEADGRDI
                     SQLVVSGELGKVNGIGAGLTEKITELVQTGKLAYYLQLKSEIPDGVLEILRVPGMGPK
                     KVRAVYTELGVDSLGSLEYACRENRLVTLPGFGSKTQQKVLEGIERLKKFRERHLLDT
                     ALIDAGKLADYIRQSPNATRVEIAGSLRRRRETIKDVDILVTAVNPEAVMNHFVAYPE
                     VETVTGHGETKSSIVLNTGINADLRVVRQDQFAYALHHFTGSREHNTEMRSRARKMGL
                     KMNEYGLFKDDEQPIECADEQSLFQELGLQYIPPELRENTGEIEAAERGEIPTLIEPG
                     DVRGIFHVHSNYSDGSMTLERIAAIGRSLGYEYVGIADHSQSARYAHGLEPERVIEQH
                     REIDEVNKGADGFRLLKGIESDILKDGSLDYSDDILEQFDFVIASVHSNFNLSEREMT
                     ERILKALRNPFVTMLGHPTGRLLLAREPYPVDLEAVLECAAENGVAVEINANPHRLDL
                     DWIHCKRAKALGVRIAICPDAHDEEGFEDTAYGVAVARRGWLERSDIINTMALTEVTA
                     WLQRQRSAKTTR"
     gene            2452..3105
                     /gene="nth"
                     /locus_tag="PKH07_02095"
     CDS             2452..3105
                     /gene="nth"
                     /locus_tag="PKH07_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009251607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HNT33767.1"
                     /translation="MAAKTKISKDHKVEQILALLQKHYPDARCHLNFKNALELLIGTI
                     LAAQCTDARVNLVTPSLFRKYPNAKAFADADEETLQDEIRSTGFFRNKAKSIRHCCQA
                     LVAQYGGEVPSDMKSLSALSGVGRKTANVVLGNYYGKPAIIVDTHMLRVAQRLGLTKE
                     TDPTRMEMSLLKIIPDKQQTLFSNRIGEHGRTICQARKPKCEECFLKDLCTFYAKKS"
     gene            3134..4627
                     /gene="pap"
                     /locus_tag="PKH07_02100"
     CDS             3134..4627
                     /gene="pap"
                     /locus_tag="PKH07_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003506793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0043751 - polyphosphate:AMP
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006797 - polyphosphate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate:AMP phosphotransferase"
                     /protein_id="HNT33768.1"
                     /translation="MLDNLDLSREVSKADYKEAKDQLELILPALQRKAKEMQVPVIII
                     FEGWSAAGKGTLINQLILPLDPRGFNVSSTLAPTVEETMRPFLWRFWNRIPASGRIAI
                     FDRSWYRHVLSERMSGRLRGTELQQAYEDIRSFERQLTDGGYVIIKFFLHISKQEQKK
                     RFSKLQGTAATKWRVTSDDLKQNKQYDKYLSALEDMLAESDSDYAPWTVVESHDQRFA
                     TLKIFNTVVSALESRMEKTQPVEKPKPTRGKKRSLPAALDASVLDTVDLSVEVDRETY
                     DQRIKKRQARLRELEHEIYVKRLPVVLVYEGWDAAGKGGNIRRVTQNLDPRGYEVVPI
                     AAPNDIERAHHYLWRFWTQIPKAGHIAIFDRSWYGRVLVERVEGFCSEHEWKRAFREI
                     NEMEHHLTNFGTVLLKFWLHIDSEEQLRRFKEREQLEHKKWKITEEDWRNRDRWDTYK
                     TAVEEMLFRTSTPHAPWTIVESNCKWFARLKVLDTVIEAIEKSLPKG"
     gene            4756..>5218
                     /locus_tag="PKH07_02105"
     CDS             4756..>5218
                     /locus_tag="PKH07_02105"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNT33769.1"
                     /translation="MKNKHDEQEHRTTRRDFLRAGSIAGLGVALGSLSLSGCASSQPQ
                     RGTFLAPGALPAGRPVRVGFVGVGNMGSAHVGNLLNIEGVQLCAICDIVEAKVARVQK
                     LVEDAGQPRPKGYSRGTLDFKRMCAEEELDLVYTATPWEWHTPVCVAAMQNG"
BASE COUNT         1281 a         1292 c         1487 g         1158 t
ORIGIN      
        1 cctctgggtc ttctgaatct gtttcttgcg ggggcggtgc cgataggtct tctgattttc
       61 acacatgttg agtcccgttc ctatgtccca ccgcgctatg ctctgtatcc tgtttttctc
      121 atgactttga tggtggcggg actcttcctg aacatccgtc acgtttcgcg tgtgatgggt
      181 tggctgttgc tcgccgcctt tcttgcgaca aactgtgtca gcgtctatca acgtcaagca
      241 catcctgtca tcaggacaac catggtggct gagaccgatc aggtgatcct tgactttctg
      301 aaggaaaggg gtatctccgc agcctgtacg agtctggatt cgcccgattc aggctactgg
      361 aacgcctacc gcttgagcgc cagttccgga gacactattt ttattcatcc aattctccac
      421 atgccgcgca ttgaacgcta tcgggctgct ttggagcggg cggagcattg tgcgatactc
      481 actcctgatc cggaaaggac ggaagtcgtt tttcagcgga acaatatcaa ctacagccga
      541 ttcgatcacg gcggcatgac agttctctgt gactttgaca aaacccatgt ggatcggctt
      601 aagctgattg actaccgtcg ttcgcttcgg gaagagcgaa cgcgatagca cgctcttcag
      661 ttgacggaat tcgggcggtc tgttctgata ggacggcaac cagaacgttg gctgtgcgcc
      721 caggcaggtc agttggatag cgcgaggctt cttcatggat cgtgaatctg taatcgcaat
      781 tctggatgag attgccatcc tgctcgagct gaagggtgag aatcccttca agtcgcgcgc
      841 ttattcgaat gcagcgcgtg ccttggaagc ggatggcagg gatatttcgc agttggttgt
      901 gtcgggggaa cttggcaaag tcaacgggat tggagctggt ctgaccgaga aaatcacgga
      961 actcgttcag acgggaaaac ttgcttacta cctgcaactc aaatcggaga ttcctgatgg
     1021 agtgctggag atattgcgtg tgcctggaat gggtccgaag aaagttcgtg cggtctatac
     1081 cgaacttgga gtggactctc tggggagtct ggaatatgcc tgtcgggaga accgccttgt
     1141 cacccttccc ggttttggct caaagacgca acagaaagtt cttgaaggga tcgaaaggct
     1201 caagaagttt cgggaacgac atctcctgga cacggctctg atcgatgcgg ggaaacttgc
     1261 cgactatatc cgacagagtc cgaacgcgac ccgggtcgag atcgcaggca gtctgcgccg
     1321 cagacgagag acgatcaaag atgttgatat tctggtcacc gccgtaaatc cagaagccgt
     1381 gatgaatcat ttcgtcgcgt atcctgaagt cgaaacggtt acaggacatg gggaaaccaa
     1441 gagcagcata gtactcaaca cgggcatcaa cgccgatctt cgagtcgttc gacaggatca
     1501 gtttgcctat gcgcttcacc acttcacggg cagtcgtgaa cacaacacgg agatgcgctc
     1561 ccgtgccagg aagatggggc tgaagatgaa cgaatatggg ctgtttaagg atgacgaaca
     1621 gccgattgaa tgcgcggatg aacagtcgct tttccaagaa ctcggtcttc agtacatccc
     1681 gccggaactg cgtgagaaca ccggcgagat cgaagcagct gaacgaggtg agattcccac
     1741 gctgattgaa ccaggcgacg tgcgtggcat ctttcatgtg cattccaact acagcgatgg
     1801 ttcgatgacg ctggagcgca ttgcagcgat cggacgatca ctgggctatg aatacgttgg
     1861 aattgcagac catagccaat cggcgcgtta cgcgcacgga ctggaacctg aacgcgtcat
     1921 tgagcagcat cgggagatag atgaagtcaa caagggtgcg gatgggttca gactcctgaa
     1981 ggggattgag tcggacattc tgaaggatgg aagtctggac tattcagatg acatactcga
     2041 gcaattcgat ttcgtcatcg cgtcggttca cagcaacttc aatctgtccg aaagagaaat
     2101 gaccgagcgc attctcaagg cattgagaaa tccttttgtc acgatgctcg gacatcccac
     2161 aggcagactt ctgctggcgc gtgagcctta ccctgttgat ctggaagcgg tgctggaatg
     2221 tgctgccgag aacggtgttg cggtggagat caatgccaac ccacaccgcc ttgatctgga
     2281 ttggattcac tgcaaacggg cgaaagccct gggtgtgcgt atcgccatct gtcccgacgc
     2341 tcatgatgaa gaaggttttg aggacaccgc ctacggtgtg gcggtggcgc gaagaggatg
     2401 gttggaaaga agcgatatca tcaacacgat ggcactgacg gaggtcacag catggctgca
     2461 aagacaaaga tcagcaaaga ccacaaggta gagcagattc tggcgctgct tcagaaacac
     2521 tacccggatg cccggtgtca tctcaatttc aagaatgccc tggagttgct tatcgggacg
     2581 attcttgccg cccaatgtac agatgctcga gtaaatctgg tgacccccag tctttttcgg
     2641 aagtatccca acgccaaggc gtttgctgat gcggatgagg aaaccctcca agacgaaatt
     2701 cgctccactg gattctttcg caacaaggcc aagtcaatcc gtcactgttg tcaggcattg
     2761 gtggcgcagt acggtggaga ggttccctcg gacatgaaga gtctttcggc actgtcaggg
     2821 gttgggagaa agacggcaaa cgtggttctg ggcaactact acggcaagcc tgcaatcatt
     2881 gtggacacgc acatgcttcg agtggcacag cgtcttggcc tgacaaaaga gacagatccg
     2941 acccgaatgg agatgagtct tctgaagatc atcccagaca aacagcagac tcttttttca
     3001 aatcgcattg gtgagcacgg cagaacgatc tgccaggcca gaaaacccaa gtgcgaggag
     3061 tgtttcctga aagacttgtg cacgttctac gcaaagaaat cgtagggtct gttgacccaa
     3121 ggaggatgtt tccatgctgg acaatctgga tctatcgcga gaagtctcca aagccgacta
     3181 caaggaagcc aaggatcaac tggagttgat cctgccggcg ctacaacgca aggccaagga
     3241 aatgcaggtt ccggtgatca tcatctttga aggatggagt gcagcgggta agggaacgct
     3301 gatcaaccaa ctcatccttc ccctggatcc tcgtggattc aatgtttcca gcacgcttgc
     3361 tccgacagtc gaagaaacca tgagaccgtt tctgtggcga ttctggaatc gcattcccgc
     3421 cagtggcagg atagcaatct tcgaccgcag ttggtaccgg catgtcctga gcgaacgcat
     3481 gagcggaaga ctcaggggca cggaacttca acaggcgtat gaagacattc gttcctttga
     3541 gcgtcagttg acggatggcg gctatgtcat catcaaattc ttcctgcaca tctcgaagca
     3601 ggaacagaaa aagcgcttct caaagttgca gggtaccgcg gcaaccaagt ggcgtgtcac
     3661 ctcagacgat ctgaaacaga acaaacagta cgacaagtat ctctcggcgc tagaggatat
     3721 gcttgccgag agtgattctg actacgcgcc ttggacggtt gtcgaatcac atgatcagcg
     3781 atttgcgacg ctcaagatat tcaacactgt ggtctccgca ttggagtcga gaatggagaa
     3841 aactcagccg gtggaaaaac caaaaccgac gcgaggcaaa aaacgcagtc ttccggcagc
     3901 cttggacgca agcgttctcg ataccgtaga cctttcggtt gaagtggatc gcgaaaccta
     3961 tgaccaaaga atcaagaagc gtcaagcgcg gctgcgtgag ctggagcacg agatctatgt
     4021 gaagcgtctt cctgttgtct tggtgtacga aggatgggat gcggcgggta aggggggaaa
     4081 cattcgtcgg gtcacgcaga acctcgatcc gagaggttat gaggtggttc caattgccgc
     4141 acccaatgat atcgaacgcg cacatcacta tctctggcga ttttggacac agattcccaa
     4201 agccggtcac atcgccattt ttgaccgctc ctggtacggt cgagtcctgg tcgaacgggt
     4261 cgagggtttc tgttctgagc atgagtggaa gagggctttt cgggagatca acgagatgga
     4321 gcaccatctg accaatttcg gtaccgtgct cctgaaattc tggctccaca ttgacagcga
     4381 agaacaactt aggcgtttta aggaacgaga gcagttggag cataaaaagt ggaaaatcac
     4441 cgaagaggac tggcgtaacc gtgaccgatg ggacacctac aagacagccg tggaggagat
     4501 gctcttccgc accagcacgc ctcatgcgcc ctggacaatt gttgagtcca actgcaaatg
     4561 gttcgctcga cttaaggttc tggacactgt catcgaagcc atcgagaaga gtttgcccaa
     4621 ggggtagagg caggtctgag acttgccatc ctgaacccca ttgtatgcta cctttgtggt
     4681 actgttcggt ctatccgaag caagaagcgt ccaatgcagg cgcggcagac atgccagagg
     4741 aagggagata actgagtgaa gaataaacac gacgaacaag aacacagaac aacacgacgt
     4801 gactttctca gggcagggag catcgcagga ttgggcgtgg ctcttggaag cctgagcctt
     4861 tcaggatgcg cctcttcaca accgcagcgg ggaacgtttc tcgccccagg tgcccttccg
     4921 gccggtcggc cggttcgggt tggtttcgtg ggagtcggaa acatgggttc cgcccacgta
     4981 ggcaatctgt tgaacattga aggagttcaa ctctgcgcga tctgcgatat tgtcgaggca
     5041 aaggttgctc gagtccagaa gctggtcgag gatgccggcc aaccccgtcc gaagggctat
     5101 tctcggggta ctctggattt caagcgcatg tgcgcggaag aagaactcga cctggtctat
     5161 accgccacgc cttgggaatg gcacactccc gtctgtgttg ccgcgatgca gaatggca
//
LOCUS       DAOQUC010000073         5195 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_17617_length_5195_cov_3.105058, whole genome shotgun sequence.
ACCESSION   DAOQUC010000073 DAOQUC010000000
VERSION     DAOQUC010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5195
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17617_length_5195_cov_3.105058"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1337)
                     /locus_tag="PKH07_02110"
     CDS             complement(<1..1337)
                     /locus_tag="PKH07_02110"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNT33770.1"
                     /translation="MTVEKPPRVLIVDDDTAIRFTLRELLRKEGYETDEASNGRMALE
                     MFDPEVHDVITLDLRMPKMGGMKILPLVFGKDPDAQVIVLTAHGGKQEALEAYEIGVR
                     DFLDKPFDNDVLRITVRRALEKRKLLREKALLNLRVTALQSEIVSRGEFDTIVGTSPA
                     MQQVRELIRSIAASDVTVLLCGESGTGKEVIAREIHRQSARASGPFIALNCAAIPETL
                     LESELFGYEKGAFTGAVEQRAGKFELAREGTLLLDEIGDMPQSTQAKILRVLQEREFQ
                     RVGGAEVVKTDIRVLAATNQDLAALVREGSFREDLYFRLNVIPIMLPLLKDRREDLVL
                     LAQHFIEKYNKQFSKKAERLGPKAEAALYAYDWPGNVRELENTIQRAVLLSNSPVIEE
                     LQIQPPPSPRTPRGQTLSDAFPTIQERMASMKEQIEREAILAALEQCRWRRAEA"
     gene            complement(1334..2851)
                     /locus_tag="PKH07_02115"
     CDS             complement(1334..2851)
                     /locus_tag="PKH07_02115"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNT33771.1"
                     /translation="MRKTRSGIELQRGRVSASVHLSVALPVLSALAVLVPGFIYRAII
                     IHEFSPEIPFTKYHLEMVLNLLVCWMLGLSLVAFFFGSVLGYIISAPLRQMSELLLRL
                     ADEGETETVSVRGGDEIGLLGHSFNRMVQSMAKYLPERARFVFHNVATGVITTNYGGM
                     LETINATADRILRLSGVGVIGRKYDRVFRPHGGYHAIWEILRETITSSRGVMDREVTI
                     RCLDGEEVALSLNTVVAVDPNTGRNAVVLTLIDLTWLKNVSRQMLQQEKLSAVGSLAA
                     GVAHEIRNPLGALRSLTQMITQSEGSETSRQEYAQVMLSEIDRLNSVVRNLLDFSSPS
                     IEELSQVQFASLIGRAVELSGIHVIKPAVEIIREDAPDTPTVLGNESRILQAILNILL
                     NAADEVSHEGKIFIYTKRTVAGDKVECTIANDGPPIPESLREKIFDPYFTTKDDGTGL
                     GLTIASQIVSQHKGTLKVSSGEGQLTAFTITLPASSQDITPAETATPKQGGTDPS"
     gene            3076..3492
                     /locus_tag="PKH07_02120"
     CDS             3076..3492
                     /locus_tag="PKH07_02120"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNT33772.1"
                     /translation="MRTSKRSDKREFSRTSVRVAVELESDDASLHCDQTRDLSMKGLF
                     LLTRDRFPLDSHCRAVLYLVECGSPLRIEVTARIVRIEEDGMAIEFLEFMEPESFEYL
                     KNLMLYNSANPTQVEKELDSHVGLRPRKRIRSKSSK"
     gene            3576..4466
                     /locus_tag="PKH07_02125"
     CDS             3576..4466
                     /locus_tag="PKH07_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006939159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HNT33773.1"
                     /translation="MSDPVKTIRIGTMVPGGKAVEAIRHLLPHGFESFEICFGNETEE
                     QLRTLAKQVGDVLKDQDAVVSCLGIYGNPLESGENDLACVAMWERLIDNAHLFGANVV
                     SGFTGRLRGKSIDSSIPRFKEVFQPLADRASVKGVRLAFENCDGDGTWRSGDWNIAHY
                     PQAWEMMFEAVPNENIGLEWEPCHQMIKLIDPMAQLRKWAKRILHVHGKDATVMWDVI
                     REYGITGSKPFFFHRTPGFGDSNWTDIISELRLSGFAGSIDIEGWHDPVYRGEMEVTG
                     QIHALNHLKRCRGGAFVPNY"
     gene            4674..>5195
                     /locus_tag="PKH07_02130"
     CDS             4674..>5195
                     /locus_tag="PKH07_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006950578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33774.1"
                     /translation="MDQDNTAVSSPHVEEPDTVHQGIEHAKGRRRSPWLWVPSAYYAE
                     GLPYIIINVVSVVMYQRMGVSNKDIAFFTSWLYLPWMLKPLWSPLVDLIKTKRWWVLV
                     MQFLMGVGLGCLAMVIPMEGWFRWTMTIFWVMAFCSSTHDIACDGFYMLGLSKHQQAW
                     FVGVRSSFYRLAML"
BASE COUNT         1170 a         1429 c         1408 g         1188 t
ORIGIN      
        1 gcctcagccc ttctccagcg acactgctcg agcgcagcga gaatagcttc ccgttcgatc
       61 tgttccttca tgctggccat acgttcctgg attgtcggga aggcatccga caaagtctgt
      121 ccacggggtg ttcgagggga gggcgggggt tgaatctgga gttcttcgat gacagggctg
      181 ttcgacagaa gcaccgcacg ctgaatcgta ttctccaatt cacgcacatt cccaggccag
      241 tcataggcat acaaggccgc ctccgccttg gggcctaatc gctcagcctt tttcgagaac
      301 tgcttgttgt acttttcgat gaaatgttgg gcaagcaaga caaggtcttc ccgccgatcc
      361 ttgagcaagg gcaacatgat gggaatcaca ttgagacgaa aatacagatc ctcccgaaaa
      421 gatccttctc gaaccaaggc cgcgagatcc tgatttgtgg cggccagcac acgaatgtcg
      481 gtcttaacta cttccgcgcc accgacacgc tgaaactcac gctcctgcaa cacccgaagt
      541 atcttcgcct gagtggactg gggcatgtca ccgatctcat ccagaagaag cgttccctcg
      601 cgcgccagtt cgaacttacc tgcacgctgc tccacagcgc ccgtaaaggc tcccttttcg
      661 tagccgaaca actcgctctc cagaagcgtc tccggaattg cggcacagtt gagcgcaatg
      721 aacggtccgg atgcacgcgc actctgacga tgaatctcac gcgcgatgac ctctttgccc
      781 gttccagact cgccgcacaa aagcacggtc acatcactgg ccgcgatgct ccgaatgagt
      841 tcgcgcacct gttgcatggc aggtgacgtg cccacgatgg tatcgaactc gccacggctg
      901 acgatctccg actgaagggc ggtgacgcgc agattcagga gcgccttttc gcgaagaagt
      961 ttcctttttt ccagtgcgcg tcgaaccgtg atgcgcagaa catcgttatc aaagggtttg
     1021 tcgagaaaat cgcgcactcc tatttcgtag gcttccaggg cttcctgctt gccaccgtgc
     1081 gcggtcagaa cgatgacctg ggcatcggga tccttgccga agaccaaggg gagtattttc
     1141 atcccgccca tcttgggcat tcgcaagtcg agtgtgatga cgtcatgcac ttcgggatcg
     1201 aacatctcca gcgccatccg gccgttgctg gcttcatccg tctcgtagcc ttccttgcgc
     1261 aacagctcac gaagcgtgaa acggattgcc gtgtcatcat ccacgatcag tactcgcggc
     1321 ggtttttcca cagtcacgat ggatcagtac ctccctgttt cggtgtggca gtttcagcgg
     1381 gagtgatatc ctgagacgac gcgggaaggg tgatcgtaaa ggcggtcaac tgtccctcgc
     1441 cgctggagac tttcagagtg cctttgtgct gcgacacgat ctgagacgca atggtcagac
     1501 ccaaacccgt accatcatcc ttggtggtga agtagggatc aaatatcttc tcccgcagtg
     1561 attcgggaat gggagggcca tcattggcga tggtacattc cactttgtct cccgcgactg
     1621 ttcttttggt gtagatgaag attttgcctt catggcttac ttcatcggca gcgtttagca
     1681 ggatgttcag aatcgcctga agaatcctgg attcgtttcc aagaaccgtg ggcgtatcgg
     1741 gagcatcctc gcggatgatc tccaccgcgg gtttgatgac atgaattccc gaaagctcga
     1801 cagctcgtcc aatcaaggac gcgaactgca cctgactgag ttcctctatc gaaggcgagg
     1861 agaaatccag gagattgcgg acaactgaat tgagccggtc tatctcactc agcatcacct
     1921 gggcgtattc ttggcgcgat gtttctgacc cctcgctctg ggtgatcatc tgagtcaggc
     1981 ttcgcagcgc gccaagcgga ttacgaattt cgtgagccac acccgccgca agagagccaa
     2041 ccgcggagag tttttcctgc tgaagcattt gacgggagac gttcttgagc catgtcagat
     2101 caatgagtgt caacaccacg gcattgcgac ccgtattggg atccacggcg accacggtgt
     2161 tcagagaaag cgcgacttcc tctccatcca gacaccgaat tgtgacttcc cgatccatca
     2221 caccacgact actggtgatc gtttcccgaa gaatctccca gatggcatgg tacccaccat
     2281 gagggcgaaa cactctgtca tatttcctgc caatcactcc cactccggaa agccgaagaa
     2341 ttcggtcggc agtcgcgttg atcgtttcca acatgccgcc atagttggtc gtaatgacac
     2401 cggtcgccac attgtgaaag acaaagcgcg cccgttcggg aaggtacttg gccatggact
     2461 gcaccatgcg gttgaacgag tgccccagaa gaccgatctc atcaccgccg cgcacagata
     2521 cggtttcggt ctccccttcg tcggccaggc gcaagaggag ttcggacatc tgtcgcaacg
     2581 gagcactgat gatgtagccc aataccgaac cgaagaagaa agccaccaga gagagcccca
     2641 acatccagca cacaagcagg ttcaagacca tctccagatg gtatttagtg aaagggattt
     2701 cgggtgaaaa ctcgtgaatg atgatcgcgc ggtaaatgaa ccccggcacc aacaccgcca
     2761 atgcactcag caccggaagt gccaccgaca gatgcaccga agcggagact cggcctcttt
     2821 gcagctcaat gcccgaacgg gtctttctca tgatcggtct ccggcaacct aagatggcgg
     2881 ctgttctccc cgaatgcaca caatggggac tgttgtttcg ggcatattag gtcatctctc
     2941 aaactggatc aaatttttca aactgttcgc accagttgca gagaaccagc ccttaaggta
     3001 gtatgatcca taccagttgc agaactttct gcgccgctgc agagcaaatg ttcagcacgc
     3061 catgggaggt ctgagatgag aacttcaaag cgatccgaca aaagagaatt ctcacggact
     3121 tcggttcgag tggccgtaga acttgagtcc gacgatgctt cccttcattg cgatcagaca
     3181 cgggatctga gcatgaaagg cctgtttctg ctgaccagag accgattccc cttggacagc
     3241 cattgccgcg cggtgctcta tctcgttgaa tgtggcagcc cgcttcgtat cgaagtgacc
     3301 gccaggattg tccgtatcga agaggatgga atggcgattg aatttctgga gttcatggaa
     3361 ccggagagtt tcgagtatct caagaacctg atgctctaca attccgctaa cccgacacag
     3421 gttgagaagg agcttgactc gcacgtcggt ctgcgtccga gaaaacgtat ccgcagcaag
     3481 tcaagcaagt aggagcatct ggtgtctttt tcagacgctc ttcgcttttg cgtcgagaga
     3541 acattttata ctacacccat tgaaaggaca ggaatatgag cgaccccgtg aagacaattc
     3601 gtatcggcac gatggtgccc ggcggaaagg ctgtcgaggc aatccgtcat cttcttcccc
     3661 atggttttga gagcttcgag atatgctttg gcaatgagac cgaggagcag ctgcgcaccc
     3721 tcgccaagca agtcggagat gtcctgaagg atcaggacgc cgtggtgagt tgtctgggca
     3781 tctatggcaa tccgctggag agcggggaga acgatctcgc gtgcgtcgca atgtgggaac
     3841 gcctcattga caacgcccac ctcttcggag ctaatgtcgt ctcgggtttc accggacggt
     3901 tgcgtgggaa atccattgat tcctcgatcc cccgattcaa ggaagtcttc cagcccctgg
     3961 cagaccgtgc ctcggtcaaa ggcgtaaggc ttgcttttga gaactgtgat ggcgacggaa
     4021 cgtggcggag cggcgactgg aacattgctc actatccgca ggcctgggag atgatgtttg
     4081 aggcggtgcc caatgaaaac atcggcctgg aatgggagcc ctgccatcag atgatcaaac
     4141 tcatagaccc tatggcacag ctgcgcaaat gggcgaagag gatacttcac gtccacggaa
     4201 aagatgctac cgttatgtgg gacgtgattc gtgaatacgg aatcaccggc tccaagccgt
     4261 ttttcttcca tcggacgcct gggttcggtg acagtaattg gacggatatc atctccgagc
     4321 ttcgcctgag cggtttcgca ggttccattg acattgaagg ctggcacgac cctgtctacc
     4381 gaggtgaaat ggaagtcacc ggccagattc atgcattgaa ccacctgaag cggtgccgtg
     4441 gtggcgcgtt tgtacccaac tactgagcaa ccggaataac ggtttcctcc tcatgcttga
     4501 gttgatgact catggactgc tgaaatgccg ggatcagatc acccatccac ggcgttactt
     4561 ggcaagacat tccgaacaga actcaaaacc ctcctgcctt gacctttgga gagtgcctgt
     4621 ctattatcat tcgggcgtgt acccgcgaac aataacctga gaagggagtc agaatggatc
     4681 aggacaacac tgccgtttcc tctccgcacg tcgaagaacc ggacacggtt catcagggta
     4741 tcgagcacgc caaggggcga cgtcgttctc cttggctgtg ggttccctcg gcttattacg
     4801 ctgaagggct tccctacatc atcatcaacg ttgtctcggt tgtgatgtac cagcgcatgg
     4861 gtgtgtccaa caaggatatc gccttcttta ccagttggct ctatctgccg tggatgctca
     4921 agcctctgtg gagtcccctg gtggatctga tcaagaccaa acgctggtgg gttctggtga
     4981 tgcagttcct catgggcgtt ggtctgggat gcctcgccat ggtgatacca atggaagggt
     5041 ggtttcgatg gacgatgacc atcttctggg tcatggcgtt ttgttcttcc acgcacgaca
     5101 tcgcctgcga tgggttctat atgctggggt tgagcaagca ccagcaggcg tggtttgtcg
     5161 gagtgcgcag ctctttttac cgcctggcga tgctg
//
LOCUS       DAOQUC010000074         5190 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_17638_length_5190_cov_2.438948, whole genome shotgun sequence.
ACCESSION   DAOQUC010000074 DAOQUC010000000
VERSION     DAOQUC010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5190
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17638_length_5190_cov_2.438948"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1369
                     /locus_tag="PKH07_02135"
     CDS             <1..1369
                     /locus_tag="PKH07_02135"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HNT33775.1"
                     /translation="LLIAWTGNMIAAFSALFFVPRFRRIGITTVSEFLDQRYGLWLRL
                     LTAVAWTLYYALFAGTAVYTLSVALEPVLNMPVNTLILIIGGVVILYCAFSGLVSVAY
                     TAIIQSFLMILGGLILLPLSLKAVGGWTSFVEQVPPDLFVFWKSGNVWPNYKDIIMFT
                     LLGFPYWCTSQYMLQRTFAGKTVEESSRGITLAALLTGPLTLSYILPGICGVLLFQGA
                     NALDKADKVLPQMIVQFMPAGLGGLFIAALVAASNSTASSLLNSIATLVDHDFFRRFV
                     PNKSPRFYIWVGRIATVIGGLIGLSFALQVADLGIFQAVYKVMGFFEPPIFVLVACAL
                     FWRFANVWGAFLGLTIGFLFNVIYSGRGMHPADMAIIDFPLCLVCVVVGSLIGRYLSP
                     ETEEAQQRMTNCWCEPVAESERTAPSRRKSALAGQSSLWQPSFCVRSLKSTFPSRETS
                     SYLWA"
     gene            1360..1560
                     /locus_tag="PKH07_02140"
     CDS             1360..1560
                     /locus_tag="PKH07_02140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33776.1"
                     /translation="MGLMMSFVLGCYHAIPLFVYGEEDKESKLAKTAIEGTWVHRFFG
                     NGYTWLCLYVLAIALVFILYFV"
     gene            1629..2942
                     /locus_tag="PKH07_02145"
     CDS             1629..2942
                     /locus_tag="PKH07_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013929876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNT33777.1"
                     /translation="MVSSKNHKSITRRSFLKQGAKIGTALLAAPWVIPASALGADGTT
                     APSNRLTLGFIGLGKMGMGHFGGCLNGSEVQVIAVCDVEALRLEIARKEVDRVYSKDG
                     GPGGGCLAFHDFRELLAREDVDAVFICTPDHWHAIQSIEAAKAGKDIYCEKPMVHTIR
                     EGRAVVDAVRRYGRVFQTGSQQRSNYGGMFRQAAELVRNGRIGEIKAIHVQVGGPGEA
                     CYLPAQPVPEGLDWDFWVGPAPWRPFNDELCPAYHVGGFPNWRGYYDFGGGGLFDFGA
                     HHFDIAQWALGMDDSGPVEIIPPDGKDYPRLTFIYANGVPMYHGGGRNDVEFIGTEGT
                     IHVGRGSIGSEPASLLSEPLTPGELRLNRNKGHKEDWLNCIRTRQRPIADVEIGHRTA
                     SVCHLGFIAYTLKRPLKWDPVKEQFINDTEADRMLGYGMRSPWHL"
     gene            2960..>5190
                     /locus_tag="PKH07_02150"
     CDS             2960..>5190
                     /locus_tag="PKH07_02150"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNT33778.1"
                     /translation="MSRFPKAFWLVALAVLCSCATPRSLRMETVPSELEAAFQQLPNY
                     RFGEPNAFVVQISNAVNTSQQSPAQRHWLEGKLLDVVKSSGTRDARDFACRQLSLIGT
                     KTSVSTLVSLLTDEELSDSARYALERIPGSAVEKSLVKSLAKVNSHTRQGILNSLGSR
                     QSARSVSAIRRALSDEDKDVVAAALYALANIGSPKSALALIGFRAQGFADMPYVYGDA
                     CLICAERLVDKGYYTGALQIYGMLISKHKSNRLMPEEIRSAALKGVYATGGMSYNANM
                     SGALFGMLHDIERLGPVADLIRESPDLFPAKDLAEALDVCEPSAQLILLGILSDRGDS
                     SVSDEVLQAVESPDASVRRAALEALTVCGDIHSLDTLIQIAATGDRQEQESARTTLSR
                     LQGEGINERLLAQIPDISPEYQVEVFRALVSRVASETRPELLKMAQSQNATIQRESFR
                     AIASLGMAEDVTPTLQALLEAQDESARKEAERTVALLCEKQPDQTGRTQIILNALADV
                     DDVTNTCSLLRIMGRLGGSQALESFRQAMESEDADIRETAIRSLSEWPDATALDDLMQ
                     RVQSLDEGLHKTLAMRGAARLIGLAQDKTSQERVALCTQLMNLAGNAGEKKLVLSVLG
                     KVPTAESLEMVTASLGDEELFSEASAALFDLSNPLKETNFKELESALDRIISATTTDD
                     NTREKAEEILVGLDRYSGHILIWEVAGPYTQEGSKGEDLLDVVFAPEQAAGDVEWKAI
                     GKQ"
BASE COUNT         1072 a         1323 c         1486 g         1309 t
ORIGIN      
        1 tttgctgatc gcatggacgg ggaacatgat cgccgctttc agcgccttgt tcttcgttcc
       61 aagattccgc cgaattggca ttacgacggt atcggagttt ctcgaccagc gctacggtct
      121 ctggcttcga ctcctgaccg cggtggcatg gactctctac tatgccctgt ttgctggcac
      181 agccgtctac acactgtctg ttgcgctgga gcctgtcttg aacatgcctg tcaacacact
      241 gattctgatc atcggcggtg tggtcattct ctactgcgcg ttctccggac tggtttccgt
      301 agcatacacc gctatcatcc agtcgtttct gatgattctg ggtggtctga tcctgttgcc
      361 attgagtttg aaggctgttg gaggttggac ttccttcgtg gagcaggttc cacctgacct
      421 gtttgtgttc tggaagtccg gaaatgtctg gcccaactat aaggacatta tcatgttcac
      481 cctgttgggt tttccctact ggtgtaccag ccagtatatg ctccagagaa ccttcgctgg
      541 caagactgtc gaggaatcct ctcgtggcat tacactggct gccctgctga ccggtccgct
      601 gacgctttct tacatacttc ctggcatctg tggtgttctg cttttccagg gcgctaatgc
      661 tcttgacaag gcggacaagg ttctgcctca gatgatcgtg cagttcatgc ccgccggatt
      721 gggtgggctg ttcattgctg ctctggttgc agcgtccaac tcgaccgcca gttcgcttct
      781 gaactccata gccaccttgg tggatcacga cttcttcaga cggtttgtgc ccaacaaatc
      841 ccctcgattc tacatctggg ttggtcgaat tgcgacggtc attggaggct tgattggctt
      901 gtcctttgcc ttgcaggttg cggacttggg gatcttccaa gcggtctaca aggtcatggg
      961 attctttgag ccgcccatct ttgttctggt cgcatgtgcc ctgttctgga gattcgccaa
     1021 tgtctgggga gcgttcttgg gtctaaccat cggatttctt tttaacgtga tctactctgg
     1081 gcgagggatg catccggcgg atatggcgat cattgatttt ccgctttgcc ttgtctgtgt
     1141 ggttgttgga agcctcatcg gcaggtatct ctcaccggag acagaggagg cgcagcagag
     1201 aatgaccaac tgctggtgcg aacccgtggc ggaatcagaa aggacagccc cgtctcggcg
     1261 aaaatcggcg ctggctgggc agtcctctct ctggcagcct tcgttctgtg tgcgttcctt
     1321 gaagagcacc ttcccaagcc gggaaacatc ctcatattta tgggcctgat gatgtctttt
     1381 gttctcggct gttatcacgc aataccgttg tttgtctatg gagaagagga caaggaatcg
     1441 aagctcgcca agacagccat cgaagggaca tgggtccacc ggtttttcgg aaacggctac
     1501 acatggctct gtctctatgt gcttgcaatc gctttggttt tcatacttta ctttgtttga
     1561 atgaatctgc ttgacggttc gtcctgtgtt cggtagtaag ggctgagcat gctcagttaa
     1621 ggagacttat ggtgtcttcc aagaatcaca aatcaatcac acggcggagt tttctgaagc
     1681 agggtgccaa gattggcaca gcgcttctgg cggcgccttg ggtgatcccc gcgtccgcgc
     1741 ttggcgcaga tggtacaacg gctccaagca atcgactgac acttggattc attggcttgg
     1801 gcaaaatggg catgggtcac ttcggtggct gtctgaacgg atcggaagtc caggtgattg
     1861 ctgtctgtga cgtggaagcc ctgcgattgg agattgcccg aaaggaagtg gatcgggtct
     1921 actcaaaaga cggcgggccc ggcgggggct gtctggcgtt tcatgatttt cgggaattgc
     1981 ttgctcggga agacgtggat gctgtcttca tctgcacacc ggatcattgg catgccattc
     2041 agagcattga ggcagccaag gctggcaagg atatctattg cgaaaagccc atggttcata
     2101 ccattcggga aggtcgcgct gtggtggatg ctgttcggcg ctacggacga gtcttccaga
     2161 cgggcagcca gcagcgctcc aactacggag gcatgttccg tcaagcggct gaactggtgc
     2221 gtaacggaag gatcggagag atcaaggcca ttcatgttca ggttgggggg cccggagagg
     2281 cgtgttatct tccggcacag ccagtccccg aagggcttga ctgggacttc tgggttggcc
     2341 cggctccgtg gcgacccttc aatgacgaac tttgtccagc gtaccacgtt ggtggctttc
     2401 caaactggag aggctactat gactttggtg ggggtggact ttttgacttt ggcgcgcatc
     2461 acttcgacat tgcacaatgg gcgctcggca tggatgacag cggtccggtg gagatcatcc
     2521 cgcctgacgg caaggactat ccgaggctga cattcatcta tgccaatggt gttccaatgt
     2581 atcatggggg tggtcgcaac gatgtcgagt tcatcgggac agaaggaacc atacatgtcg
     2641 gtcgtggaag catcgggtcg gaacctgcct ctttgctttc cgagcccctt acccctggcg
     2701 agcttcgctt gaacagaaac aaaggacata aagaagattg gctcaactgc attcgaaccc
     2761 ggcagcgccc cattgcggat gttgagattg gtcatcgaac tgcgagtgtc tgccatcttg
     2821 gtttcatcgc gtacaccctg aaacgtcctc tcaagtggga tccggtgaaa gaacagttca
     2881 tcaacgacac tgaggcggac agaatgcttg gctatggaat gagaagtccc tggcacctgt
     2941 gaaagaaagg aacggacaaa tgagtcgttt tccgaaagcc ttctggctgg tagcactcgc
     3001 tgtgctttgc tcctgtgcaa cccctcgttc gctgagaatg gagacagtgc cttcggaact
     3061 ggaggctgcg tttcagcaac tccccaacta tcggtttgga gagccgaatg cttttgtggt
     3121 tcagatttct aacgccgtca acacctctca gcagagtccg gcacaacgcc actggcttga
     3181 gggaaaacta cttgatgttg tcaagtcttc aggaactcga gacgccagag attttgcctg
     3241 tcggcagttg tctttgattg gaaccaagac atccgtctca actctggtct cacttctgac
     3301 tgacgaagaa ctctccgaca gcgcccgata cgcattggag cgaattcctg gcagcgcggt
     3361 tgagaagtct ttggtgaagt cgcttgcaaa ggtgaactcc cacacgaggc aggggattct
     3421 caactctctc ggttcgagac agtccgcacg cagtgtctca gcaattcggc gtgcattgag
     3481 cgatgaggat aaagatgtgg tcgccgccgc tctctatgcc ctggccaaca tcggcagccc
     3541 caagtcggcg ctggctctga tcggttttcg agcacaggga tttgccgata tgccttacgt
     3601 ctatggcgat gcctgtctga tctgtgctga acggcttgtg gacaagggct attacactgg
     3661 agcgcttcag atatacggaa tgctcataag caagcacaag tccaacaggc tgatgcctga
     3721 ggaaatccgt tctgccgctc tgaagggtgt ctacgcgaca ggcgggatga gttacaacgc
     3781 gaatatgtcg ggtgctcttt tcggaatgct ccacgacata gagaggttgg ggcctgttgc
     3841 cgatctgatt cgagagtctc cagacttgtt tcctgccaag gatctggcag aagcgcttga
     3901 cgtctgtgag ccttcagcgc aactcatcct gctcggaata ctctcagacc gcggcgactc
     3961 ctcggtcagc gatgaggttc ttcaggctgt cgagagtccc gacgcaagtg tgcgccgtgc
     4021 cgccttggaa gctttgactg tttgtgggga tattcactcc cttgacacgc tgattcagat
     4081 cgctgccacg ggcgacaggc aggagcagga atcagctcgt accactctgt cacgccttca
     4141 aggggaaggg atcaatgaac gtctccttgc tcagattcca gatatctctc ctgaatacca
     4201 ggtggaagtc tttcgtgcac tggtgagtcg tgttgcttct gagacccgtc ccgaattgct
     4261 gaagatggca cagagtcaga acgcaacgat tcagcgggaa tcatttcgcg ccatcgcttc
     4321 cttgggcatg gcagaggacg tcactcccac tctgcaggct ctcctcgaag cccaggatga
     4381 gagcgcccgc aaggaagccg agcgaacggt ggctctgttg tgtgagaagc agccagatca
     4441 gactggccgt acgcagatta tccttaatgc tcttgctgat gttgacgacg ttaccaatac
     4501 ctgttccttg cttcgaatca tgggcagatt aggaggatcc caggccttgg agtcgttcag
     4561 gcaagcgatg gaatccgaag atgcggatat tcgcgaaacc gcaattcgaa gtctgtctga
     4621 atggcctgat gccaccgcat tggacgatct gatgcagcgg gttcagagcc ttgatgaagg
     4681 tcttcacaag actctggcca tgcgaggtgc tgcgagactc atcggtcttg cccaggacaa
     4741 aacatctcaa gagcgtgtcg ctctctgcac tcagttgatg aatctggcag gcaatgcggg
     4801 ggagaagaag cttgtccttt cagtgcttgg caaagttccg actgccgagt cattggagat
     4861 ggtcactgcg tccttgggag acgaggaact gttctcagag gcctcagccg cactgtttga
     4921 tctgtcgaat cctttgaagg agacgaactt taaggagctg gagtccgctt tggatcgcat
     4981 tatctccgcc actacgaccg acgacaacac ccgtgagaag gccgaagaga ttctggtggg
     5041 actggatcgt tattcgggac atatcctgat ttgggaagta gcgggtcctt atactcaaga
     5101 gggctccaaa ggcgaagatc ttcttgatgt tgtgtttgct ccagagcaag ccgccggaga
     5161 tgttgaatgg aaagccatcg ggaagcagga
//
LOCUS       DAOQUC010000075         5168 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_17749_length_5168_cov_2.795815, whole genome shotgun sequence.
ACCESSION   DAOQUC010000075 DAOQUC010000000
VERSION     DAOQUC010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5168
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17749_length_5168_cov_2.795815"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            511..2733
                     /locus_tag="PKH07_02155"
     CDS             511..2733
                     /locus_tag="PKH07_02155"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33779.1"
                     /translation="MLTEQEALQRAETGIKAYLESEVASGNIDQNYFQLAQDNVIPNL
                     KNWLEDSDADRFSPNIREGIVNAIDRQRWAQLTEAYVGDIRFGTGGIRGKMAFDRESV
                     LMMKEQGIAAPILKGPNTLNDKVLLLKSVGVAQFAMERGLEKVVIGYDSRVRGADFAR
                     LIARLFLAYGLEVYLFDDACPYPEVTFAVPSLKADIGILISASHNDYRYNGYKLSCGN
                     GSQFDVKDRNYIYDKYIVNARSEHIRLIELDDAGGRQLWFLGGIEPRRGFNYHGHEKN
                     LIDMHTRHINHVKSFLIQSELIASQKETEGRLRIGFSAFHGAGRLAVPRILSEVGLSD
                     IKKIHKLDELDGTFPCFCSDPGKEQQPDPGDPRAARIAVAAFKEEYSEEGFQNIDVLI
                     GTDPDADRCGVVVKVPENQRSIYGGEDYTLLPADYAWALLLWYRLNLEKEKGTLEPEK
                     KFMVMSHTTSDLNAKVAEKFGLGVMKTWVGFAMLANAVRIVWDRETIPNLREGRTDPS
                     QSLCHPVLYECYGMNNGQRSFNVAAMEQSNGFSLLGGRPKDEYSLGEGGHVRDKDGTF
                     AALLMAEVAAYAKSQGSSLLELLDEKVFLDPEIGLFVNYYEPAPMDGEYEGLAGYTLK
                     RRILVEAENLLKASQSGETVVIGGLPVTSSVIHLTGKYDKQNWPGFPDEGIRFYFDQE
                     RFDWLHIRPSGTSNALRFHVQLRGKDVNKENLIALKQTLAQRAEAIVQDIRTKIRAQA
                     "
     gene            2854..>5168
                     /locus_tag="PKH07_02160"
     CDS             2854..>5168
                     /locus_tag="PKH07_02160"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HNT33780.1"
                     /translation="MIGGTVVKRLLYLAICLLSSVLLAADCAHADSTFFHQDDFRSYP
                     KDSDGSPAWETLGISWTAGNGRFECVDPGKDFALLSGSPWGELGHFEVTLVLKESISS
                     DWGVAGLGFCVDRDHYWHYAFVEQPDEKGKGRFVELAQMFAGQWNAQASGATELKAAE
                     IIGQDFRWSYNTPYRMRIEWNSNSVRGTILDMKGVLLSRISYRFGFQPGCRVGKPMLV
                     SGGFRSHFSDVQARISGREDYAMAQPTPFPEYTVKGNRRVQGERTGYFHVKKEDGRWW
                     LFDPKGNGFFMVGTDHCNYRVHWCEKLGYAPYHEFVSEKYPSEEAWADNAVKRLLKWN
                     FNSLGANNSPSTRYKGLAYTEFLGVGQGFVNHDDICPRTTWTGFPNVFNPRFREFCRK
                     QASQRCAPLMTDPWLLGYFIDNELEWFGKSHKPWGLCDEVFKKPSDHTAKRAFAGFVQ
                     ERHKDIESFNKAWGTTLTSFDAILDVTSPIDTMTELGLQDKKDFVRLIAEKYFEITTT
                     SIREYDPNHLILGTRFAGQNPSIWDIAGKYCDVVSVNCYRTLDLETEEFTDGFPEDLL
                     QWYEQCKKPLMITEWSYPALDAGLPCKYGAGQRVDTQAQRAKAYSIFQRKVFSLPFMV
                     GSMFFMWVDEPALGIASTFPEDSNYGLVNERDEEYPELTQTATRINKAVYDLHEGRTS
                     QLRAEFKSAGVLEVGNHGRADARTSVVIWMNGNRTKRTLRVKSGQSQTVNLALPKNLG
                     GVFTQAVIDPTRLLIEPDAVGHRAQLIEYNSG"
BASE COUNT         1274 a         1207 c         1436 g         1251 t
ORIGIN      
        1 caagtgggta gtttcggaat cacgacaaca tggggtgcat aactctcatc ccaaagccct
       61 gtgacctccg cactcgcagc aacagggact ataaaaacag ccaaaatgac aaacaaccta
      121 aggtctttca tagccaatcc tccttgagtc catactcatt catcgtcttt tctcggccgt
      181 cgtcaagcat cagaaggcca tgagttcctg caaagaaggc cgaacaattg atgggacaaa
      241 ccaacaactt gttcagcagc tctacctcaa gtggttgatg ggtacatact cccatcaaaa
      301 tcaggttgtg ggtatcctaa cagaaaagtt gtcgaattgc gacaaatgca aagactcaag
      361 acaatccatc gatcttccta tcaaacggtc atggaaattg ccctttttgc tggacgatct
      421 cggatggaat ggtgtattaa agagtggata gagtgcttca gaaaagtcat tcataggcga
      481 agcggacgac aaaccaggag gtaatcacga atgttgaccg aacaggaagc ccttcagaga
      541 gctgaaactg gcatcaaagc gtatcttgaa tctgaggttg cgtccggcaa catagatcag
      601 aactactttc agttggcgca ggacaatgtg attcccaacc tgaagaactg gctggaagat
      661 tctgacgctg accgcttctc ccccaacatc cgagagggaa ttgtgaacgc gattgatcga
      721 cagcgttggg cacaactgac cgaagcctat gttggtgata ttcgcttcgg taccggtggt
      781 attcgaggaa agatggcttt tgatcgtgag tcggttctta tgatgaaaga gcaagggatt
      841 gctgctccga ttctcaaagg tcccaatacg ctcaacgata aggtgctcct gctgaagtcg
      901 gtgggtgtgg ctcagtttgc catggagagg ggtttggaga aggtagtcat cggttacgac
      961 agtcgagttc gtggcgcaga cttcgcgcgc cttattgctc gtctattttt ggcgtatggc
     1021 ttggaagtct atctgttcga tgacgcgtgc ccctatcccg aagtcacgtt tgccgttccg
     1081 agtctcaagg cggacattgg catcctgatc tcggcaagcc acaatgatta ccggtataat
     1141 ggctacaaac tctcctgtgg aaatggttcg cagttcgacg tgaaagatcg caactacatc
     1201 tacgacaagt acatcgtgaa tgccaggagc gagcatattc ggctcattga actggacgat
     1261 gcaggaggca gacaactgtg gtttctcgga ggaattgaac cccgccgagg attcaactat
     1321 cacggccatg agaagaatct cattgatatg cacactcgcc acataaatca tgtgaagagt
     1381 tttcttatcc agagtgagtt gatcgccagt caaaaggaga cggaaggccg attgcgtatc
     1441 gggttcagtg ccttccatgg tgctggaaga ctggccgttc cgagaattct ctctgaggtt
     1501 ggcctttctg acatcaagaa gatacacaaa ctggacgagt tggatggaac gtttccctgc
     1561 ttctgcagtg acccaggcaa ggaacagcaa cctgatccag gcgatccaag agctgcgaga
     1621 attgcggttg cggcgttcaa ggaagagtat tcagaggagg gttttcaaaa cattgatgtg
     1681 ctcatcggaa cagatccgga cgccgaccgg tgtggggtgg tcgtgaaggt tcccgagaat
     1741 cagcgttcta tctatggtgg cgaagactat accctgctgc cggcagacta tgcgtgggcg
     1801 cttctgttgt ggtaccggct caatctggaa aaagagaagg gaactcttga gcccgagaag
     1861 aagttcatgg tcatgtctca tacgacaagt gatctgaatg cgaaagttgc tgaaaaattc
     1921 ggactcggag tcatgaagac ctgggtcggg tttgcgatgc ttgccaatgc cgttcggatc
     1981 gtctgggatc gggagacaat tccaaatctt cgagaaggac gcaccgatcc gagtcaatct
     2041 ctttgtcatc ccgtgctgta tgaatgctat ggcatgaaca acgggcaacg atctttcaac
     2101 gttgccgcga tggaacagag caacggcttt tcgcttcttg gtggccgccc gaaggatgag
     2161 tattctctcg gagagggagg acatgttcgt gacaaagatg gcacgtttgc ggcgctgttg
     2221 atggctgagg tggcagcgta tgcaaagtcc caaggttcgt ctcttctgga actgcttgac
     2281 gagaaggtgt tccttgatcc ggagattggc cttttcgtga attactacga acctgctccg
     2341 atggacgggg agtatgaggg tctcgcgggt tacacactta aacgtcgtat tctggttgag
     2401 gcggaaaatc tcctgaaggc gagtcaaagt ggggagaccg tcgtcattgg tggtctgccg
     2461 gtgacgtcca gcgtgattca cctaaccgga aagtatgaca agcagaactg gcctggattt
     2521 cccgacgaag ggattcgatt ctactttgac caggaacgat ttgactggct tcatatccgg
     2581 ccatccggca cctcgaatgc tcttcgtttc cacgttcaat tgagaggcaa ggacgtgaac
     2641 aaggaaaacc tcatcgcact caagcagaca ctggctcaac gtgctgaggc aatagttcaa
     2701 gacattcgca ccaagattag agcgcaggcc tgagcgttgg cctgtctttc aaagccatgg
     2761 atttctggag tgggtgactg ctgacgagcc tgtgttgctc tcgtttgatc ggtgcccggc
     2821 ttccagtttg gaaatacact ccatggaaac tccgtgattg ggggtactgt cgtgaagcga
     2881 ttactctatc tagccatttg ccttctcagt agtgtgctgc ttgctgctga ctgtgcccat
     2941 gccgactcaa cgtttttcca tcaagacgat ttcaggtctt atcccaagga ctccgacggc
     3001 tctcctgcgt gggaaacgct tgggatcagt tggacggctg gcaatgggcg atttgagtgt
     3061 gttgatccag gcaaggattt tgccctgctg tctggatcgc catggggcga actggggcat
     3121 ttcgaagtca cactcgtttt gaaggagtcc atttcatcag actggggggt ggctggtttg
     3181 ggattctgtg ttgatagaga tcactactgg cactacgcct ttgtcgaaca acccgacgag
     3241 aagggaaaag gtcgctttgt cgaactggct caaatgtttg ccggacagtg gaatgcccag
     3301 gcttcaggtg ccactgaact caaagcggcg gagatcattg gtcaggattt ccgatggagt
     3361 tacaacactc cttaccggat gcgaatcgag tggaactcca acagtgttcg cgggacgatc
     3421 ctggatatga aaggcgttct cttgagtaga atttcttacc ggtttggttt ccagcccggt
     3481 tgccgtgttg gcaaaccaat gcttgtgagc ggtggatttc gctcccattt ctctgacgtt
     3541 caggcacgga tttcgggcag agaggattac gcgatggcac aaccaacccc atttccagaa
     3601 tatactgtga agggcaatcg acgtgttcag ggcgaacgaa caggttattt ccacgtcaag
     3661 aaggaagacg gtcgctggtg gctttttgat cccaagggga atggtttttt catggttgga
     3721 accgatcact gcaactaccg agtccactgg tgcgagaaac tgggctatgc gccctaccac
     3781 gagtttgttt ccgagaagta tcccagcgaa gaggcatggg cggacaatgc agtcaagcga
     3841 ctcctgaaat ggaatttcaa ctcgctgggt gcaaacaact cgccttcgac tcggtacaaa
     3901 ggcttggcgt atacggagtt tcttggtgtg ggtcagggct ttgtgaacca tgacgacatc
     3961 tgtccgagga cgacctggac tggttttccg aatgttttca acccaaggtt tcgggagttc
     4021 tgccgaaaac aggccagcca gcgctgcgct cctctgatga ctgatccttg gctgctcgga
     4081 tatttcattg acaacgaact ggagtggttt ggcaagtcgc acaagccgtg gggactctgt
     4141 gacgaggttt tcaagaaacc gtccgaccac acggccaaga gggcatttgc tggttttgtc
     4201 caggagcgac ataaggacat tgagagtttc aacaaagcgt ggggaacgac actgacatcc
     4261 tttgatgcga tactggatgt cacaagtccc atagacacca tgacggagct ggggcttcag
     4321 gacaagaagg atttcgtgcg actgattgcc gagaaatact ttgagattac gacgacaagc
     4381 attcgagaat atgatccgaa tcatctcata cttggcacac gattcgcggg tcagaatccc
     4441 tcaatctggg acattgcagg caagtactgt gatgtggtca gtgtcaattg ctacaggaca
     4501 cttgatctgg agacggaaga gttcacggat ggctttcccg aggatcttct gcaatggtat
     4561 gaacagtgca agaaacctct gatgatcaca gagtggagtt acccggctct ggatgcagga
     4621 cttccgtgca agtacggtgc tggtcagaga gttgatacgc aggcacagcg tgccaaggcg
     4681 tacagcatct tccagcggaa ggtcttctca ctgccattca tggtaggttc catgttcttc
     4741 atgtgggtgg atgagcctgc gcttggtatt gcgtccacat ttcctgaaga cagcaactac
     4801 ggtcttgtca atgaacgtga cgaagagtat ccagaactga cccagactgc gacgagaatc
     4861 aacaaagcag tgtacgatct tcacgaaggc aggacatcac aactccgcgc cgagttcaag
     4921 agtgctggcg ttcttgaagt cggtaatcac ggcagagctg atgcacgaac gagcgttgta
     4981 atctggatga atggcaacag gacgaagcga actctccgtg tgaaatctgg ccagtctcag
     5041 actgtcaatc ttgcgcttcc aaagaacctt ggtggagttt ttacccaggc ggtgatcgat
     5101 cccacaaggc tcctgatcga acctgatgcc gtgggtcatc gggcgcagtt gatcgagtac
     5161 aattcagg
//
LOCUS       DAOQUC010000076         5167 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_17752_length_5167_cov_2.988459, whole genome shotgun sequence.
ACCESSION   DAOQUC010000076 DAOQUC010000000
VERSION     DAOQUC010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5167
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17752_length_5167_cov_2.988459"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..317)
                     /locus_tag="PKH07_02165"
     CDS             complement(<1..317)
                     /locus_tag="PKH07_02165"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HNT33781.1"
                     /translation="MRRTIVEISVGIFVLLGLICVAYLTIKLGKMEWLGTGNYYQVKA
                     RFGNIGGLKKGAVVDMAGVQIGQVIQVNLDPEDNTAVVTMKIRNDLQLTEDVIASIKT
                     AGL"
     gene            complement(354..1103)
                     /locus_tag="PKH07_02170"
     CDS             complement(354..1103)
                     /locus_tag="PKH07_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019439515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNT33782.1"
                     /translation="MGKAVLDIRNVHKSFGSQQVLAGVNLVIEQGLITTIIGRSGVGK
                     SVLLKHIAGLIPPDKGDILFNGRSLVRLKGPVRRVFKRHFGYMFQNNALFDSMTVFEN
                     IALPLQERTSLSQDEIRERVMNIVGQLDLQEVPHKYPSQISGGMCKRVALARALITEP
                     EIILFDEPATGLDPIRKNAVYQMITRCQRDFGFTAMIVSHDIPDVFYISQKVAMLEKG
                     KIIFEGTHEEIIRCEEPVVRHFIEGKETYED"
     gene            complement(1110..1889)
                     /locus_tag="PKH07_02175"
     CDS             complement(1110..1889)
                     /locus_tag="PKH07_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaE family lipid ABC transporter permease
                     subunit"
                     /protein_id="HNT33783.1"
                     /translation="MPVTLLGRFVIDTIQEMGRIALFFSNTLLRIFAPPIKLEHLFQQ
                     IHFIGMKSLLIICLTALFTGMVLGLQGYYTLVKFGSEALLGTAVALTLIRELGPALTA
                     LMVTGRAGSSMAAEIGIMRISEQIDALDTMDIEPIRFLISPKMAACLVSFPLLTAIFD
                     VVGIIGGYLTGVVLLGINAGTFFTRMEMGVLMRDITGGFIKSLVFALIVATICCYQGY
                     TTHTRRGGFGARGVSQATTSAVVISSVLILVSDYVLTSFLL"
     gene            complement(2093..4681)
                     /locus_tag="PKH07_02180"
     CDS             complement(2093..4681)
                     /locus_tag="PKH07_02180"
                     /inference="COORDINATES: protein motif:HMM:NF014852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 20 zincin-like fold
                     domain-containing protein"
                     /protein_id="HNT33784.1"
                     /translation="MGTRLFLLLLVLALLASRSHASGLPRTIVPHPKELTWKDSDPVE
                     IHVNEAAIVLGRSASEQEQYAAELLRDSVSRRFGQDWPVSTEDTMPNTSRVLILMGQR
                     TTNSALDACCKAIKSKLSTDWPGHDGYIIETIQESDRLVVIAGGSNDRAVIYAADTLF
                     QLLESSDSRSTIKMHLASIHDWPTVPWRGRPQTNVENHFGQGVLDAYARSRINFTDLR
                     NGIYAFPADYQFTESDRQKIGKVIQECHRRGLLVYGSVDVGIPRSLHEQALKRFAEFI
                     EMGVDGLWLSFDDHGPGEAAETIVSRVLELGSQHNIKDHLIATTPPKGSYQRILRDDH
                     GDRPGFNKAIASVPGMEKALWFFTCLPSEEAIQEAQSIGLKVRPAWWHNWPRANSGFT
                     HSSTAPSIKGIPCYGEVFPLSAGWHQPSDSYLVANGHCIEAIMPWGGGGWDHYWVIPQ
                     IGWLGWSPETYDWREIQSRVFDIVFGPSKPGHVQEFNDCLITAERLCRFPLWGFKYKP
                     QSPPRLKDIGDRPELNRLLDQMENLLIQIRASAEQGGLLDTDHLENRFLKPMQQVIQI
                     GRSVSALSFPEHWYEPVQRQILQALYVEDHKSAQVLIDQAKPRILNEIDMISQSLDIH
                     NATANYVQWWRQWAQMNVEDWDALIHQRREELLQFVWRYNYYQVLRKELLKGVHDFPD
                     GWGVGGGKSGPIILGKFLPSNREYFNGTWYAGVFNDTENNTDLVAFLQSMDCANDEPD
                     NYAELELTLPVGNSGRNLALMFFMNRDVKNKVGHYYSREMWAGHSFLEIILDNKVIWE
                     EDIGLPRQGREWSLVSLPALPEKTEEVRIRLRIQDRKRAPGMRAFVLMSPVHLVEIPA
                     DTIDRY"
     gene            complement(4703..>5167)
                     /locus_tag="PKH07_02185"
     CDS             complement(4703..>5167)
                     /locus_tag="PKH07_02185"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HNT33785.1"
                     /translation="TCYSAVHLKHPLATLTIIGSNKGLFGVRFGKLPLVGLARDASPI
                     QTAVQQILCYLDGKPITQHIQIDLASATPFQKEVWKITQTIPFGETRSYAWVASQLGN
                     PKKARAIGQAMSANPIPIVIPCHRVIASDGSLGGFSAGLKWKQILLDHESRH"
BASE COUNT         1248 a         1356 c         1327 g         1236 t
ORIGIN      
        1 ataagaccag cggtcttgat cgaagcaata acgtcttcgg tcagttgaag gtcattgcga
       61 atcttcatgg tcactaccgc tgtgttatct tctggatcaa gatttacctg aatgacctga
      121 ccgatctgaa ctcctgccat gtccaccacg gcacccttct tcaaaccccc gatgtttcca
      181 aaccgtgcct tgacctgata gtagttgccg gttcccaacc attccatctt gcccaactta
      241 atggtcagat acgcgacgca gatcagacca agcagtacga atatgccgac ggatatttca
      301 acgatggttc ttctcatata agcctcttgc gactcaagcc ttcttcaggc cttctagtct
      361 tcataggttt ccttgccctc tatgaaatgc ctgactacag gttcttcgca ccggataatt
      421 tcctcatgcg ttccttcgaa aattatcttt cccttttcca acatagcaac tttctgggaa
      481 atgtaaaaca catcaggaat gtcatgactg acaatcatgg cggtaaagcc aaagtctctc
      541 tggcagcgag tgatcatctg atagacggca tttttgcgaa tcggatccaa gccggttgca
      601 ggttcatcga aaaggatgat ctccggctct gtgatcagcg cccgagccag agcgacgcgc
      661 ttgcacattc cacccgagat ttgtgaaggg tatttgtgag gaacttcctg gagatcgagc
      721 tggccaacga tgttcatcac gcgctcccgt atctcgtcct gggagaggct tgtgcgttcc
      781 tgaagaggca aagcaatgtt ctcaaatacg gtcatggagt cgaacagggc attgttctga
      841 aacatgtagc caaaatgtct cttgaagacc ctccgaacag gacccttgag tcgtacaaga
      901 ctccgtccat tgaacaagat atcacccttg tccggtggaa tcagaccggc aatgtgtttc
      961 aacagaacgc tctttccaac accgcttcgg ccgatgatgg ttgtaatcag cccctgttca
     1021 atgacaaggt tgacgcctgc aaggacctgc tgggatccga aagacttatg tacatttctt
     1081 atgtccagta ctgccttacc catagttcct cagagcagaa aagatgtcag aacatagtcg
     1141 ctgacgagaa tcagaacgct ggagatgacc acggcagaag tagtggcttg gctgactcct
     1201 cgtgccccaa agccgcctcg gcgtgtatgc gttgtgtaac cctggtagca acagattgtt
     1261 gccacgatca gtgcaaacac gagagacttg atgaatccac ccgtgatgtc cctcatgagt
     1321 acacccattt ccattcgtgt gaagaaagtg cccgcattga tgcccagaag gacaacccct
     1381 gtgagatagc caccgataat cccaaccacg tcgaaaatgg cggtcagaag gggaaaactg
     1441 acaagacatg cagccatctt tggcgaaatg aggaatcgga tcggctcgat atccatcgta
     1501 tccaaggcgt ctatctgttc cgatatccgc atgattccaa tctcagcagc cattgaggat
     1561 ccagccctcc ccgtgaccat caaggcagtc aaggcgggac caagttcgcg gatcagggtc
     1621 agcgccactg cggttcccaa gagtgcttcg gaaccaaact tgaccaaggt gtaatagccc
     1681 tgaaggccaa gaaccattcc cgtgaacagg gcggtcaagc agatgatcag aagggatttc
     1741 attccgatga agtggatctg ctggaaaagg tgttccagtt tgatgggtgg ggcgaaaatc
     1801 ctcaacaagg tgttgctgaa gaacaacgca attcgtccca tctcctggat cgtgtctatg
     1861 acaaaacgac ccaagagagt tactggcatg cacatggaac aattggcttt ttccatcgtt
     1921 atgttcaagc tgttcctgta tcaatctcct ggataaccag actccgaaag atgtggtttc
     1981 tttcgcgagc aagcaccacg ctcgccctct ttcggaaagc gaaatctcta taacagcaag
     2041 ataatctacg gagaggagcg gactttgtca aggtatgctg actgccactg ttctaatagc
     2101 ggtcaatggt gtcagccgga atctccacga gatggacagg actcatcaag acaaaggctc
     2161 tcattcccgg tgcacgcttt ctatcctgaa ttctcaagcg aatccgaact tcctcggtct
     2221 tttccgggag agctgggaga gaaaccaaag accactctcg tccctgtctg ggcagcccga
     2281 tgtcctcttc ccagatcact ttgttgtcca ggatgatctc aaggaaagaa tggcctgccc
     2341 acatttcgcg tgagtaatag tgtccaacct tgttcttgac gtcgcgattc ataaagaaca
     2401 tcagggcaag attccggccg gagtttccta cgggcagcgt cagttccaat tcggcatagt
     2461 tgtctggttc atcattggca cagtccatgc tctgaagaaa ggcaacaaga tcggtgttgt
     2521 tctccgtgtc attgaagaca cctgcatacc aggttccgtt gaagtactct cggttcgagg
     2581 gaaggaattt ccccagaatg ataggacctg atttgccccc accgacaccc cagccgtctg
     2641 gaaaatcatg aacacccttc agaagctcct tgcgaagtac ctggtagtag ttgtatcgcc
     2701 agacgaactg aagcaattcc tcacgtctct gatgaatcag agcatcccag tcctcgacat
     2761 tcatctgtgc ccattgacgc caccattgga catagttggc tgtggcattg tgaatgtcga
     2821 gcgattggga aatcatatct atctcattga gaattcttgg ctttgcctga tcaatgagta
     2881 cttgagcact tttgtggtcc tcgacataga gggcttggag aatctgacgt tggacaggtt
     2941 cgtaccaatg ctcagggaag gacagagccg acactgagcg tccaatctgg atcacctgct
     3001 gcattggttt caggaaacgg ttttccagat ggtcggtgtc cagaaggccg ccttgttcgg
     3061 cagacgccct gatctgaatc agaagattct ccatctggtc gagtagacgg ttgagttccg
     3121 gacggtcccc aatgtctttc agccgtggtg gtgactgggg tttgtacttg aagccccaca
     3181 aggggaagcg gcaaagacgc tcggcagtga tcagacagtc gttgaactcc tggacatgtc
     3241 ctggttttga gggtccgaag acgatatcga acacgcggct ttgaatctcc ctccaatcat
     3301 aggtttccgg agaccatcca agccagccaa tttgcgggat gacccaatag tgatcccaac
     3361 ctccaccacc ccaaggcata atcgcttcta tgcaatgtcc gttggcaacc agataggaat
     3421 ctgacggctg atgccatcct gctgacaagg gaaagacttc accgtagcac gggatgcctt
     3481 tgatcgacgg tgcagtggac gaatgggtga atccgctgtt ggctcttggc cagttgtgcc
     3541 accaagcagg acgcactttg agaccgattg actgtgcttc ctgaatggct tcttcagaag
     3601 gaaggcacgt aaaaaaccag agtgccttct ccatgcctgg caccgaggca atagccttgt
     3661 tgaaacccgg tctgtcacca tgatcgtcac gaaggatgcg ttggtaggag cccttcggtg
     3721 gggtcgtggc gatcaggtga tctttgatgt tatgttggct gccaagctcc agtacccggc
     3781 tgactattgt ctccgcggcc tctccaggcc cgtgatcatc gaatgacagc cagagaccgt
     3841 ccacccccat ttcaatgaac tcggcgaacc gcttcagcgc ctgctcgtgc agtgagcgtg
     3901 gtatgccaac gtcaacagaa ccatagacca gaaggccgcg gcgatggcac tcctgaatga
     3961 ccttgccgat tttctgtcga tccgattcgg tgaactgata gtcggcaggg aatgcgtaga
     4021 tgccattgcg caggtcggtg aagttgatgc ggcttcgggc gtaggcatcc agaactccct
     4081 ggccaaagtg attctcgaca ttggtctgag ggcgtccccg ccaaggcacc gttggccagt
     4141 catgaattga ggcaaggtgc atctttatgg tggaacgact gtccgacgat tccagcagtt
     4201 ggaagagggt gtccgcggcg tagataaccg ctcggtcgtt gcttccccct gcaatgacca
     4261 ccagcctgtc ggattcctga atggtctcaa taatgtagcc atcgtgtcca ggccagtcgg
     4321 tgctgagctt gctcttgatc gctttgcagc aagcatccag agcgctattt gttgttctct
     4381 gtcccatcag gatcagaaca cgtgatgtgt tcggcattgt atcttctgtg gagaccggcc
     4441 agtcctgacc aaatcgcctg gaaaccgagt cccgaaggag ttccgcagcg tactgctcct
     4501 gctccgacgc ggaacggcca agcacaatgg cagcctcgtt cacatggatc tccaccggat
     4561 cggagtcttt ccaggtgagt tccttcggat gtggaacaat tgtccgcggc agtccggagg
     4621 catgtgacct ggaagcgagg agagccaata ccagaagcaa cagaaagaga cgagtgccca
     4681 tacgcttacc tttcctcgaa cctcagtgac gagattcgtg atcaagtaga atttgcttcc
     4741 acttcagacc cgcagagaaa ccaccgagtg aaccatctga agcaatgacc ctgtgacagg
     4801 gaattacgat gggaataggg tttgcggaca tcgcttgtcc gatggcacgt gccttctttg
     4861 ggttgcccag ctgcgatgcg acccacgcat aggaacgggt ctcaccgaag gggattgtct
     4921 gcgtgatttt ccacacctct ttctggaagg gtgttgccga tgccaaatcg atctggatgt
     4981 gttgggttat gggtttgccg tccaggtaac agagaatctg ctggacagcg gtttgaatag
     5041 ggcttgcgtc cctggcaaga ccgacaagag gcagcttccc aaaacgaacc ccaaaaagcc
     5101 ccttgttgga gccgatgatt gtaagtgttg ccagtggatg cttcagatga actgctgaat
     5161 agcacgt
//
LOCUS       DAOQUC010000077         5144 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_17855_length_5144_cov_3.604441, whole genome shotgun sequence.
ACCESSION   DAOQUC010000077 DAOQUC010000000
VERSION     DAOQUC010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5144
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17855_length_5144_cov_3.604441"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..370
                     /locus_tag="PKH07_02190"
     CDS             <1..370
                     /locus_tag="PKH07_02190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HNT33786.1"
                     /translation="KTKPDTLQVKEFKRPHQAPLDPERCFLDQGFLCLGPVTRCGCDS
                     ACINGNMPCSGCLGPLDGIRDYGAKALSAFASILDFAEEQDIENALDAIADPGGTFYR
                     YSLPASLMHRRLTRGDGESQ"
     gene            367..1839
                     /locus_tag="PKH07_02195"
     CDS             367..1839
                     /locus_tag="PKH07_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HNT33787.1"
                     /translation="MKKKVTIDPITRLEGHGKIDIFLNDQGDVDRAYLQIPELRGFEK
                     FAEGRLAEDMPQITSRICGVCPTAHHMAATKALDALYRVQPTSAARKIRELVNSTFFF
                     EDHALHFFFLGGPDFVVGPQAEKKDRNILGVLGKVGLETGKEVIATRRELRDLGSRAG
                     GKVIHPVLGLPGGVSKRLTKEDQEKFIQGAQKALSLADKSLDLFHSIVLKNQEYVDLI
                     TSDAYTHKTYYMGLVDDNNKVNFYDGRVRVVTPDGKEFLRFPASDFAKHVAEHVEPWS
                     YIKFCYLKEVGWKGFVDGADSGVYAVAPLARLNASEGMATPKAQQAYEEFYSTLGGKP
                     VHLTLANHWARLVEMVYAAERMAELANDPEITSSEIRNLPTEVPEEGVGIVEAPRGTL
                     FHHYATDSRGVISKANLIVATQNNAARIALSVDKAAKGAIRNGQINDGLLNLIEMGFR
                     AYDPCHGCASHALPGQMPLIIRVLDAQFNILQEIRQNRDL"
     gene            1836..2285
                     /locus_tag="PKH07_02200"
     CDS             1836..2285
                     /locus_tag="PKH07_02200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HNT33788.1"
                     /translation="MRILVLGLGNDLLADDAIGILASRRLSPLVQGTADVQETSVAGL
                     ALLDFLIGYDRAIILDAVCTGRHAPGTILRLTPDDLAPAFSPSPHYSGLPEVLTLALE
                     MALDVPKDIVILAVEVEDVTTLGGEMSVPVTKALDEIVQRVQTEIGV"
     gene            2354..3487
                     /gene="wecB"
                     /locus_tag="PKH07_02205"
     CDS             2354..3487
                     /gene="wecB"
                     /locus_tag="PKH07_02205"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HNT33789.1"
                     /translation="MQLIFVFGTRPEAIKMAPLVLEARKQSPQVVSEVIVTAQHRQML
                     DDVLDAFSIEPDVDLDLMAQGQSLPQLTARICQELEPLFRNRQPDYVVVQGDTTTAFA
                     AALCAFYAGIKVAHVEAGLRTGKKWAPFPEEMNRRMTSCLTDIHFAPTESAKQNLLRE
                     GYPEHTIHVTGNPVIDALQWMGSRLEGTPYPFQENEIPLDRFRRFVLITGHRRESFGE
                     PFRRICAALRTLAENNPDVLFVYPVHLNPNVQEPVYQALSDSSNILLLEPLNYPQFVW
                     LMQRSYLIITDSGGIQEEAPALGKPVVVTRNVTERPEAVEAGVVKLVGDDTSAIIATV
                     QGLLSDPNVYRAMARGVSPYGDGKAASRIVETLKTRAEASAEE"
     gene            3494..>4733
                     /locus_tag="PKH07_02210"
     CDS             3494..>4733
                     /locus_tag="PKH07_02210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33790.1"
                     /translation="MSQVRPDIWGQGILFAFSGWDGTTDWFEDFVLIAEEKPFVWRVE
                     GTVSFVFQIRTALQDNLSLVSADVIANDHLAAVFEDTKGSRVEIKMAMRDCHTLVGSV
                     TLLDDAAEIVLSVAKLEPPVRVECDIEALRVRHGDKARVVFLERSDVSLEEDGYHLTT
                     QAEKGDCVLFCVGKSVPNNFSAAVLREAFETRESFFTRLPDAPGAFLDPDPINRMKAL
                     LKAASVIKVNLHTAQGQIPYNWSTPDRYPHRKMWLWDSAFHTIGLRFLDPDLAGDQVK
                     AVLSQMQDNGFIPHYSCPAGETSNITQAPILAWASLAVFLEHHEKTFLRDVYPQLRQF
                     LEFFLRERDRDGDGLCEWEFRHASGMDNSPRFDEGSSFDAVDLNCFLARDFDAMRWIA
                     KALENEFPGDADMWFERHQTI"
     assembly_gap    4734..4743
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4744..>5144
                     /locus_tag="PKH07_02215"
     CDS             <4744..>5144
                     /locus_tag="PKH07_02215"
                     /inference="COORDINATES: protein motif:HMM:NF013377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase family glycosidase"
                     /protein_id="HNT33791.1"
                     /translation="SARINELLWDETTGFYYDHAIDGGLAKLKTHCGFLPLFAQVAPT
                     ERAERLLEHLQDESEFWTPLPVPSVSRDDATYSKDMWRGPTWLNYNYFIIEGLHNYGF
                     AEAANALTERTLNEVVRWYLKEGVIFEYYDA"
BASE COUNT         1141 a         1369 c         1445 g         1179 t
ORIGIN      
        1 aaagaccaaa ccggacactt tgcaggtgaa agagttcaag cggcctcatc aagcaccctt
       61 ggatccagaa cggtgttttc tcgatcaggg cttcctctgt ctcggtccgg tcacccgctg
      121 tggctgtgac agtgcctgca tcaatgggaa catgccctgc tcagggtgct tgggaccgct
      181 ggacggtatt cgggactatg gagccaaggc attgtcggca tttgcttcaa tcctggactt
      241 cgccgaggag caggatattg agaatgcgct ggacgccatc gcggatccgg gtggtacctt
      301 ctatcgctac agcctgcctg cttccctgat gcaccgacga ctgaccagag gggacgggga
      361 gtcacaatga aaaagaaagt cacaatagat cccatcactc gacttgaggg acacggcaag
      421 attgatatct tcctgaacga tcagggagat gtcgatcgag cgtaccttca gatcccggaa
      481 ctgcgcggtt ttgagaagtt tgcggaaggg cgtctcgcag aagacatgcc tcagatcact
      541 tcaaggatct gcggggtctg cccgactgcg catcacatgg ctgccaccaa ggctcttgat
      601 gcactgtatc gcgtgcaacc aacgtctgcc gcgcgaaaga ttcgagaact ggtcaactca
      661 actttcttct tcgaagacca tgcgctgcat ttcttcttcc tcggcggtcc ggattttgtc
      721 gttgggcctc aagcagagaa aaaggatcga aatatccttg gcgtactcgg caaggtgggc
      781 ctggagacag gcaaggaagt tattgccacg cgaagagaac ttcgtgatct aggctcccgt
      841 gcggggggga aggttattca cccggttcta ggccttcccg gtggcgtgtc aaaacgcctg
      901 acgaaagagg atcaggagaa gttcattcag ggcgcgcaga aagccctgag cctcgcggac
      961 aagagtcttg atctctttca ctccatcgtg ctcaagaatc aggaatatgt tgatctgatc
     1021 acctcagacg cgtacaccca caagacctat tacatggggc ttgtggatga caacaacaaa
     1081 gtcaacttct atgacggcag agttcgtgtt gtaactcccg acgggaaaga gttcctacga
     1141 ttccctgcct cggattttgc caagcacgtc gcagaacatg tggaaccgtg gagttatatc
     1201 aagttctgct acctgaagga agttggctgg aaggggtttg tggatggtgc cgacagcgga
     1261 gtctatgccg ttgctccact ggcgcgtctg aacgcatccg aaggcatggc aacgcccaag
     1321 gcacagcagg catacgagga gttctatagc acgctcggtg gcaagcctgt ccatctgacc
     1381 cttgccaacc actgggcgcg actcgtcgag atggtctacg ccgcagagcg catggcggaa
     1441 ctggccaacg atccggagat caccagcagc gaaatccgaa atctgccaac ggaggttcct
     1501 gaggaagggg ttggcattgt cgaggctcca cgcggaacgc tttttcatca ctacgcgacc
     1561 gattctcgcg gagttatcag caaagccaat ttgatcgttg ccacgcagaa caacgcggcg
     1621 cgaatcgcac tctccgtgga caaggcagcc aaaggcgcta ttcgcaacgg gcagatcaac
     1681 gatggcctgc tgaacctgat cgagatggga tttcgggctt acgatccgtg ccacgggtgc
     1741 gcctcacatg cacttccggg acagatgcct ctcatcatcc gagtgctgga tgcacagttc
     1801 aatatccttc aagagatccg tcagaatcga gatctatgag aatccttgtt cttggactcg
     1861 gaaatgactt gcttgcagac gacgccattg gaattcttgc gtcgcgaaga ctgagtcctc
     1921 tcgtgcaggg aacggccgac gttcaggaaa cctctgtcgc tgggctggcg ctgctggatt
     1981 ttctgatcgg ctatgatcgg gcgatcatcc tggacgcggt ctgtacgggt cggcacgctc
     2041 caggaaccat ccttcgtttg actcccgacg atcttgctcc cgcgttctca ccatctcccc
     2101 actactccgg tctgccggaa gttctaacac ttgcgctgga gatggctctt gacgttccaa
     2161 aggatattgt gattctggcc gttgaagttg aggacgtgac cacccttggt ggcgagatga
     2221 gtgtgcctgt taccaaagcg cttgacgaga tcgtccaaag ggttcagact gaaatcggcg
     2281 tctgaggagt tttgatatca acttggatat gcgaccgtaa cagggcttga gtttgaccag
     2341 aaccatgaaa acactgcaac tgatattcgt tttcgggaca cgcccagagg cgataaagat
     2401 ggctcctctg gtgctggagg cacgcaagca gtcgccgcag gtcgtgtctg aagtcatcgt
     2461 cactgcgcag caccgacaga tgcttgacga tgtgctcgac gccttttcca tagagcctga
     2521 tgtggatctg gacttgatgg cgcaaggaca gtcgctgcct cagttgactg cccgaatctg
     2581 tcaggagctg gagcctcttt ttcgtaacag acaacccgac tatgttgtgg tgcaggggga
     2641 cacgacgaca gcatttgccg cggcactttg cgctttctac gccggcatca aggtcgccca
     2701 tgtcgaagca ggacttcgaa ccggaaagaa atgggcgcct ttccctgaag agatgaaccg
     2761 tcggatgacc tcgtgtctga cggacatcca cttcgcgccg accgaaagcg cgaaacagaa
     2821 tcttctgcgc gaagggtatc cagagcacac catccatgtg accggtaatc ctgtcattga
     2881 tgcattgcag tggatgggaa gccgactgga gggcactcca tatcccttcc aggagaacga
     2941 gattccgctg gacagattcc gcagatttgt tttgatcacg ggacaccgtc gagagagttt
     3001 tggcgaaccc tttcgacgaa tctgcgcagc gttgcgaact ctggcggaaa ataatccgga
     3061 cgtgttgttt gtctatccgg ttcatctcaa tcccaatgtt caggagccgg tatatcaggc
     3121 tctgtcggat tcgagcaata tccttctctt ggagccactc aactatcctc agtttgtctg
     3181 gctcatgcag cgaagttatc tcatcatcac cgattcgggt ggaattcagg aagaagcgcc
     3241 ggcgttgggc aaacccgtgg ttgtgactcg aaacgtgacg gagcgtccgg aagccgtcga
     3301 ggcgggtgtc gtcaaactcg ttggcgatga cacgagtgca atcattgcca ccgttcaggg
     3361 tctgctttcc gacccgaacg tctatcgggc gatggcacgt ggcgtcagcc catacggaga
     3421 cggaaaagcc gcttcacgca tcgtcgagac tttgaagaca agagccgaag caagcgctga
     3481 ggagtgagac acgatgagcc aagtgcgtcc ggacatctgg ggtcagggaa ttctctttgc
     3541 cttctcgggc tgggatggca cgacagactg gtttgaggat ttcgtgctta ttgctgagga
     3601 gaagcccttt gtatggcgcg ttgaaggcac cgtgagtttt gttttccaaa tccgaacagc
     3661 cctgcaggac aatctgagcc tggtgtccgc cgacgtgatt gccaacgatc atcttgctgc
     3721 tgtttttgaa gacaccaaag gttctcgggt cgagatcaag atggcgatga gggattgcca
     3781 cacactcgtg ggatcggtca cccttctcga tgacgcggca gagattgtcc tatccgttgc
     3841 caagctcgaa cctcccgttc gagttgaatg tgacatagaa gccctcagag tgcgtcatgg
     3901 tgacaaggcg cgcgtcgtct tcctggaacg gtcggatgtt tccctcgaag aagatgggta
     3961 ccacctcacc acacaggcgg aaaaggggga ctgtgtgctt ttctgtgttg gaaagtctgt
     4021 tcccaataac ttcagcgcgg ccgtgctgcg ggaagccttc gagacaaggg agtccttctt
     4081 tacccgtctg cctgatgcac ccggagcttt tcttgatccg gatccgatta accggatgaa
     4141 agcgcttctc aaggctgcgt ccgtgattaa ggttaatctc cacaccgcgc agggacagat
     4201 tccgtacaac tggtctaccc cggatcgtta cccgcatcgg aagatgtggt tgtgggactc
     4261 ggcctttcat accatcggac ttcgttttct tgatcccgat ctggcaggcg atcaggtcaa
     4321 ggccgtgttg tcccagatgc aggacaatgg attcatccct cattattcat gtcccgccgg
     4381 tgagaccagc aacatcacgc aggcaccgat cctcgcctgg gcttcactgg ctgtctttct
     4441 cgagcatcat gagaagactt ttctgcgaga tgtctatccg cagttgcgtc agtttctgga
     4501 attctttctc cgtgaacggg atcgcgacgg agacggactg tgcgagtggg agttccgaca
     4561 tgcttccgga atggataact cgccccgatt cgatgaggga tcgtcgtttg atgccgttga
     4621 tctgaactgc tttctcgccc gggattttga cgcgatgcgg tggatcgcca aggctcttga
     4681 gaatgagttc ccaggcgacg cagacatgtg gttcgagcgg catcagacca tttnnnnnnn
     4741 nnntccgctc gaatcaacga actgctctgg gacgagacaa ctggattcta ctatgaccac
     4801 gcaattgacg gcgggcttgc taagttgaag acgcactgcg gtttcctccc cctgtttgcc
     4861 caagtagccc cgacggagcg cgccgagcgt ttgcttgaac atcttcaaga tgaatcggaa
     4921 ttctggacgc cactccccgt tccgagcgtt tcccgagatg acgcaaccta ttcgaaagac
     4981 atgtggcgcg gtcccacgtg gctcaactac aactacttca tcatcgaagg gcttcacaac
     5041 tatggttttg ccgaagcggc caacgcattg acagagcgca ctctcaatga agtcgtgcgt
     5101 tggtatctca aggaaggggt catcttcgaa tactacgatg cgga
//
LOCUS       DAOQUC010000078         5120 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_17967_length_5120_cov_3.296150, whole genome shotgun sequence.
ACCESSION   DAOQUC010000078 DAOQUC010000000
VERSION     DAOQUC010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5120)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5120)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5120
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17967_length_5120_cov_3.296150"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1613)
                     /locus_tag="PKH07_02220"
     CDS             complement(<1..1613)
                     /locus_tag="PKH07_02220"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HNT33792.1"
                     /translation="MFKSCLFLVVVFLCMNGSFALAAEKEIRGVWVTRFEWTSEDPEI
                     CKENIREIFSSLGESNFNTALFQVRGSAETLYPSTLEPWSRLLGEKDPGFDPMQMAID
                     EARKNGVAIHAYINPMPLRETRWTEPPKDPKHIYHSHGPNSPDSWLCVDANGTPNTSE
                     YYYMSAGIPEVQAYLRTVIMDLVRRYDLDGIHLDRIRYPGPEYSHDPVSKSRFRGRGN
                     PNKLEWSDWQREQLDKFINDLAAEIRAEKPNVLLSCSAWGIYNRYHLDGYWNFSSGYH
                     DYYQDTWNWCRLGAMDFLVPMIYWNMPEPKPNYDELVKDFVKGVGGDHLVGGQRIFSA
                     TENANQITFTREAGALGTVMFSHSSARRRGIMDACKETLYQEKAEVPTFKRVTEPESG
                     TILGTVTAEDGKPLVDAWVTLRASEGSRGRRFGGGSRTWTSGADGRFAFLNVMPGNAQ
                     VRVSYPGASEILSETLEIKAGAVTKIDITVPDAKTVREEPFIDILSPSDGLETTDSVI
                     HLLGRTSPDCHVTVMDSTVEVYSTGAFAR"
     gene            complement(1857..2852)
                     /locus_tag="PKH07_02225"
     CDS             complement(1857..2852)
                     /locus_tag="PKH07_02225"
                     /inference="COORDINATES: protein motif:HMM:NF014686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HNT33793.1"
                     /translation="MNILITGAGGGSGIYSIKVLKETTDHYVIGADASPYSAGLFLAD
                     EGYVFPVARQQQAFLDRVHETVIAKDIDVIIPNVDEELPLFARPDVQSTLGAKVIVSP
                     LETILICASKQETIRRLESVVPCPRTLSGGDDLSGIAFPVHIKPDNGRGSRHTYIVED
                     AIALEECFRQLEKHLGDRRNILIQEYLPGTEYTVDVLCDLSGTPLVVVPRVRLKTSAG
                     LSIQGRTIRHTGLIENVYAIAKSLAFHGPVNMQFREDVSGVPRLLEINPRFSGGLPIV
                     CAAGANTPALLLELLQNRPIEWPVVQQSWREGIVLRYLEEFFIPWDEWQDQFTDS"
     gene            complement(2855..3952)
                     /locus_tag="PKH07_02230"
     CDS             complement(2855..3952)
                     /locus_tag="PKH07_02230"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNT33794.1"
                     /translation="MYSQGIAETASSEFSKKGHPLMPGVSVIVSTCGDASRLRETIEA
                     LIEQDCEPWQFEIIVLDDLATLTDLSNPNLPVRRIRCLDTSRVGQIALGAGVARFPLI
                     AFLESGQIPRFDWISKTVPFFVDSTVYGVRGRLVTEQTETPARVQQVIYDERCLATAR
                     EGVFSRCDSFVGIYRTRTLCASVIQSFSDAFEPDVLISETIKRMGGRIVFADLAVVFI
                     RRASTLGDLWRMNCRPRFWKSLLENDLLDGQEQEVIKEAMYFLTPSVLTLLLMLLGFW
                     SGIFHLLAVLAFLWLFGRIWSKAKFLAERSFLRPHEIAVPLFVGALAEGVGFVFSFLQ
                     TCWSGLPKWSARIYGAVAGWLTASDVNGRAK"
     gene            complement(3930..4220)
                     /locus_tag="PKH07_02235"
     CDS             complement(3930..4220)
                     /locus_tag="PKH07_02235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33795.1"
                     /translation="MDCPSSLIVYDEKTVWRCPKLGGSVPFKYCRTLLQGMPCPSVQS
                     CWENLIAITDFLEQNYSAEKLERVWKEQPKAPKVDQLFDLIQKAKDVQSRNR"
     gene            4676..>5120
                     /locus_tag="PKH07_02240"
     CDS             4676..>5120
                     /locus_tag="PKH07_02240"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF015647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HNT33796.1"
                     /translation="MKRRYMDEPRSRVIGLMSGTSVDSIDAALCDLWEEDGRIRTILL
                     GFHEHPMPIGLRERIFRVFEDGVGSLSLTCSLNFEIGNAFADAALALIEKTGIPRESV
                     TAIASHGQTAYHIAPHMVQGRNESGNVASTLQIGEPSIIAQRTGLP"
BASE COUNT         1232 a         1407 c         1295 g         1186 t
ORIGIN      
        1 tccctcgcga aagctcctgt cgagtacact tcaactgttg aatccataac agtgacatga
       61 cagtcgggtg aggttcgacc aagaagatgg atcaccgaat cggtcgtctc caatccatcc
      121 gatggagaga gaatgtcaat gaacggttct tcccgaacag tcttcgcgtc gggaaccgtg
      181 atgtcaatct ttgtcactgc ccccgccttg atttcgagtg tctcactgag aatctcggat
      241 gcacccggat atgaaacccg aacttgggca ttgcccggca tgacgttcaa aaatgcaaat
      301 cttccatccg caccacttgt ccatgttctt gaaccaccac cgaatctcct gcctctggag
      361 ccttccgatg cacggagtgt cacccaagcg tcaacaagag gcttgccatc ttcagcagtc
      421 actgttccaa ggattgtccc ggattctggt tcggtcactc gcttgaaggt tggaacttcg
      481 gccttctcct gatacagagt ttccttgcag gcatccatga ttccacggcg cctggccgat
      541 gagtgggaga acatgaccgt tcccaatgct ccggcttcac gcgtgaaggt aatctggttt
      601 gcattctccg tggctgagaa gattctctgc ccaccgacca gatggtcgcc ccccacgccc
      661 ttgacgaaat ccttgaccag ctcatcgtag ttgggttttg gttcaggcat gttccagtag
      721 atcattggaa ccagaaaatc catggcacct agacggcacc agttccaggt atcctggtaa
      781 tagtcatggt agccgctgct gaaattccaa tagccgtcca gatgatagcg gttgtagata
      841 ccccaggcag agcaggacag aagaacattc ggtttctcgg cacgaatctc agctgccaaa
      901 tcattgatga acttgtcgag ttgttcccgc tgccagtcac tccactccaa cttgtttgga
      961 ttgcctcggc cgcgaaaccg actcttgctg accggatcat gactatactc gggacccgga
     1021 tagcgaattc gatccagatg aatcccatcc agatcgtagc ggcggacaag atccatgatg
     1081 acggtgcgca gataggcctg tacttcggga atgcccgcag acatgtaata gtactctgat
     1141 gtgttgggag tgccgttggc atctacacaa agccaactgt ctggcgagtt cggcccgtga
     1201 gaatggtaaa tgtgcttcgg atctttggga ggctccgtcc agcgtgtttc gcgcaacggc
     1261 atggggttga tgtaggcatg aatcgcaacg ccattctttc gcgcttcgtc gatcgccatt
     1321 tgcatcgggt caaagcctgg atccttttcc cccaagaggc gcgaccacgg ctccagcgta
     1381 ctgggataga gcgtctctgc ggaaccgcga acctgaaaca aggccgtgtt gaaattgctc
     1441 tctcccagac tgctgaatat ctcccggatg ttctctttgc agatttcggg atcttctgaa
     1501 gtccattcaa accgagtcac ccagacacct cgaatttcct tctcggcggc aagggcaaag
     1561 gaaccgttca tacacaggaa gactactacc agaaacaaac aggacttgaa catcaccaga
     1621 acctccttga ttctccattc gatgacttca agatacctca tgtccggatg agatgcaagg
     1681 agagggatgg ccgtgtgtgg tagcaaagcg agaatgtgcc ctctattggg attacaggct
     1741 gagactcgaa gaatccgggg atgaggatga tgtccaccat ggaagaaaag tctcttgttt
     1801 ggccgggact ttcctggtgt gtttggcgtt ctattggtag agaacagtcc gattgcctag
     1861 gaatcggtga actggtcttg ccattcatcc catgggatga agaactcctc caggtagcga
     1921 agtacgattc cttcgcgcca ggactgttgg acaacaggcc attcgatcgg acggttctga
     1981 agcagttcga gcaggagcgc aggagtgttg gctcccgcgg cgcagacgat ggggagtcca
     2041 ccggagaacc tcggattaat ctccaaaagg cgcggtacac ccgaaacgtc ctctcgaaac
     2101 tgcatattca caggcccatg aaacgcgagg gatttcgcta tggcataaac attttcgatc
     2161 aagcccgtgt ggcgaattgt tcttccttgg atcgaaagcc ccgcactggt tttgaggcgc
     2221 acacgtggaa cgacaaccag aggcgtccca ctcaaatcac acaacacatc caccgtgtat
     2281 tctgtgccgg gaaggtactc ctgaatcaga atgtttcgcc tgtcgccgag gtgtttttcc
     2341 aactgccgaa agcattcctc aagcgcaatc gcatcctcaa cgatgtaggt gtgtcgtgaa
     2401 cctctgccgt tgtccggttt gatgtgaaca ggaaaggcta ttccggagag atcatcacca
     2461 ccgctcagag tccttgggca gggcaccacg gactcgagcc ggcgaatggt ttcctgtttg
     2521 ctggcacaaa tcagaatggt ctccagcggt gagacaatca cctttgcacc cagagtgctc
     2581 tgcacatccg gtcgcgcaaa gagagggagc tcctcatcca cgtttggaat gatgacgtca
     2641 atgtccttag cgatgaccgt ctcgtgaacg cgatcgagga aagcctgttg ctgacgcgca
     2701 accggaaata cgtatccttc gtctgccaga aatagtccgg cagaataggg agaggcatcc
     2761 gcgccaatga catagtgatc cgttgtctct ttgagaacct tgatcgagta tattcctgat
     2821 ccgccaccgg ctccggtgat gaggatgttc atgcttactt tgccctcccg ttcacatctg
     2881 aggctgtcag ccatcctgcc acagcgccat aaatccgagc actccacttc ggaagcccac
     2941 tccagcaagt ttggagaaaa ctgaacacaa agccaactcc ttcagccagg gctccaacaa
     3001 acaaggggac tgcaatctcg tgcggacgga gaaaggagcg ctcagcgaga aactttgcct
     3061 ttgaccatat ccttccgaag agccaaagaa acgccaggac ggccaagaga tgaaaaatgc
     3121 cgctccaaaa gcctagcagc atgagaagca aagtcagaac agagggtgtc aaaaaataca
     3181 tcgcctcttt gatgacttct tgttcctgtc cgtccaggag atcgttttca agaagactct
     3241 tccagaatct cggtcggcag ttcatacgcc acagatcacc aagagtactt gctcgcctga
     3301 taaagacaac cgctagatcg gcaaagacaa ttcgtcctcc catacgcttg atagtctctg
     3361 aaatcagcac atcgggttca aaggcgtccg agaagctctg aatcactgag gcacagagcg
     3421 tcctcgtgcg atatatcccc acaaaactgt cgcaacggct aaaaacaccc tctctcgccg
     3481 tcgcgagaca acgctcgtcg tagatgactt gctggactct ggcgggtgtt tccgtctgct
     3541 cggtgaccag tcgtcccctg acaccgtata cggtggaatc cacgaaaaac ggcaccgtct
     3601 ttgagatcca atcgaaacgc gggatctgcc ccgattcgag gaatgcaatg agagggaatc
     3661 gcgccactcc agcaccaagt gcgatctgtc ccactctgct tgtatccaga cagcggattc
     3721 gtcggactgg cagattggga ttgctcagat cagtcagcgt tgcgaggtca tcaagcacaa
     3781 tgatctcgaa ctgccaaggc tcgcagtcct gctcaataag ggcttcgatg gtttcgcgaa
     3841 gacgggaggc gtccccgcag gtagagacga tcacggacac acctggcatc aatggatggc
     3901 ctttcttcga gaactcagat gaagcggttt cagcgattcc ttgactgtac atctttcgcc
     3961 ttctggatca aatcgaaaag ttgatccact ttaggtgctt tgggctgctc tttccagact
     4021 ctttccagtt tttcggctga gtagttctgc tccaaaaaat cagtgattgc aatcaggttc
     4081 tcccagcagc tttgaacact gggacaaggc attccctgca gcagcgttct gcagtatttg
     4141 aagggaacgg aaccaccgag tttcggacaa cgccagaccg tcttttcgtc atacacaatg
     4201 aggcttgaag gacaatccat cggtttacca cctcctgtat gatgcttcac atgcagtttc
     4261 tcaaccgcgt ttctcagccc agcaactgcc gtgactctcg ctcatttcca ccccgccatc
     4321 ggaacttggt gaacagactc actattgctt cacaactgcc tctgtacttt ccaaccgatg
     4381 atagcacaac tcgcgctgtc cgcgtcaatc acttccggca ggttagcaca tgcaggcatg
     4441 tgggctatgc ccactcccac aaacgcttgt tcattggaag aaacagaatt ccaagtctga
     4501 tttaccagga gcaggttagc acctgcaagc gtgcgggcta tgcccactcc cgcaaatgct
     4561 agttcattgg aagaaacagg cttccaagtc tgatttccta ggagtattct gctctcatta
     4621 tatggttcag gtaaatgtta gaaattctgt gagcgatcct gcctgggagg tagaagtgaa
     4681 gagacgctac atggatgagc ctcgttcgcg cgttatcggc ctgatgtctg gcaccagcgt
     4741 tgactccatt gacgcggcgc tgtgcgatct ctgggaagaa gacggcagaa taaggacgat
     4801 tctgcttggg tttcatgagc accccatgcc tataggactg agagagcgga tattccgggt
     4861 ctttgaggat ggcgtcggct ctctttctct cacctgttca ctgaacttcg aaatcggaaa
     4921 cgcctttgcg gatgcagcgc tcgcgctcat tgagaagacc ggcatcccaa gagagtccgt
     4981 caccgcgatc gcttctcatg gtcagactgc ctaccatatc gctccacaca tggttcaagg
     5041 ccggaatgaa tctggaaacg ttgcctcaac actccagatc ggcgagccga gcatcatcgc
     5101 gcagcgaaca gggctgcccg
//
LOCUS       DAOQUC010000079         5081 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_18176_length_5081_cov_3.277557, whole genome shotgun sequence.
ACCESSION   DAOQUC010000079 DAOQUC010000000
VERSION     DAOQUC010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5081
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18176_length_5081_cov_3.277557"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2272)
                     /locus_tag="PKH07_02245"
     CDS             complement(<1..2272)
                     /locus_tag="PKH07_02245"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HNT33797.1"
                     /translation="MSKKDRKYVAYVVPQTHWDRAWYLSFEHFRIRLVRLTENLLKIL
                     EKNPKYRFTFDGQTVVLEDVLQVRPEFEKALTRHVKSKALSVGPWYVLPDEFLVSGEA
                     LVRNLLIGHQVAKRFGRVMKAGYIPDPFGHVSQLPQILKGFGIDSVLFMRGIGEEGDN
                     LNTEFRWIAPDGETAVIACHLIGGYGNLHSWGRIHGVALEDERVDFDQAVEHARNAIN
                     HLIAHRPTTNVLLLNNGVDHLPAQPSVPAMIKYTNEKLDNCKLIQGDYEEYVEALKKA
                     KPRLKPHQGELHNGRYFPLLSGVFSARMYLKQANERCLRLLEAYAEPLSAMNFVLGGQ
                     YPGGFLTYAWKETLKNHPHDDICGCSIDAVHRDMVSRFAHSRQVSEALTDQALGALTA
                     NTNTANGPKGAAVVAFNPSTWQRNEPVRIRTAVPAELIADGKATVVDGSGKTMHAKTV
                     VHPVREQHHWRDGKLEKSLVVDVDAEFMAEALPSFGLKTFYVTAARGKDPKPSVTARK
                     TGLENEFFRLSVNKNGTLKVEDKSTRKVYPSLHLIEDIEDVGDEYDYSPLPQEATETF
                     LSTKCGTVSSVKVLEDLGFKATLGIEQTLRIPKEVTPKRKRSSAKVAVSVKTLVSLSA
                     GSRRIDFTTTINNTAEDHRMRVLFPTGICSDKVYAAGKFDVLERSLNVPKGEGWCQPP
                     VPTKHVDGFVSVNDNRFGFTVINRGLPEYEAQKGKDGVTLCQTLFRSVEWLSRGDMQT
                     RSGHAGRCGQAVQRPGP"
     gene            complement(2400..2837)
                     /locus_tag="PKH07_02250"
     CDS             complement(2400..2837)
                     /locus_tag="PKH07_02250"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNT33798.1"
                     /translation="MNRFVVDASVVAKWIFPEEHAKESSCLVREQTSLHAPDFLSIEL
                     HNILSKKVRKKDIPPESESNILRQMEFLPVQFHLSSELLRGAYDLSVRFHTSLYDCLY
                     LSLALHLDCPLVTADARFQKSFQKTLLGATVIHVLDVQHESPS"
     gene            complement(2834..3067)
                     /locus_tag="PKH07_02255"
     CDS             complement(2834..3067)
                     /locus_tag="PKH07_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraY domain-containing protein"
                     /protein_id="HNT33799.1"
                     /translation="MAQLLVRDLDEKTVARLKKRAKANGRSLQAEAKRILEESSTVFD
                     IDRIREDLARLRASLPPQTTDSVDLIREDRDNR"
     gene            complement(3160..3303)
                     /locus_tag="PKH07_02260"
     CDS             complement(3160..3303)
                     /locus_tag="PKH07_02260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33800.1"
                     /translation="MMADGYDAEQMIGELKNQFSSSLPSLSFYFSMPILKKMQEWQQR
                     GVL"
     gene            complement(3303..3656)
                     /locus_tag="PKH07_02265"
     CDS             complement(3303..3656)
                     /locus_tag="PKH07_02265"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HNT33801.1"
                     /translation="MPRDERAYLLDIIEACQAINDAVAGFDFSCYENSRLVRSAVERE
                     FIVIGEAMAALSRFAPETFDLITRGRRIVDFRNQLTHEYHTVDDAIVWAVIKNYVPLL
                     GRESKERLVRLEEAT"
     gene            complement(3646..3954)
                     /locus_tag="PKH07_02270"
     CDS             complement(3646..3954)
                     /locus_tag="PKH07_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HNT33802.1"
                     /translation="MTRFLESRQEQISDICARHGVSKLYLFGSAAGDQFRVGESDVDL
                     LVEFQPMDACARVDAYFGMLEELRELLNVDVDLVVDGAVKNPYIAAEIERTKETLYAA
                     "
     gene            complement(4110..4997)
                     /locus_tag="PKH07_02275"
     CDS             complement(4110..4997)
                     /locus_tag="PKH07_02275"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HNT33803.1"
                     /translation="MSLHEHLLAELLTPELEQSDFEGSVEKFGKRYFDYYNAGFAISL
                     PDNPMGNLRFTSTEVLEFLEAPCERVVVHLNSFHRKQDFDQMLETADRLGVQYLLCIS
                     GDGNPKLHRLEPEELGLEPTPMGAATSVELLQYIRREYPNRFQCGVAFNPYEQPDIEL
                     MKLEKKMEVGFDFIGTQLYLPNGLVPGFDYHTPMEALTKCNKPVVFGIWTPSHTVGDV
                     SEKAMHRLSVFLECISPYYEPPVVDALKEKLMADGYDAEQMIGELKNQFSSSLPGLSF
                     YFSMPILKKMQEWQQRGVL"
BASE COUNT         1229 a         1421 c         1241 g         1190 t
ORIGIN      
        1 gcgggcctgg acgctggacg gcctggccgc atcggccggc atgtcccgat cgcgtctgca
       61 tatcccctcg tgagagccat tcgactgagc ggaacaacgt ctgacacaaa gtgacgccat
      121 ccttgccctt ctgcgcttca tactccggca agcctcggtt gattaccgtg aatccgaacc
      181 tgttgtcatt gacagaaaca aaaccatcaa cgtgcttggt gggaacgggt ggctgacacc
      241 aaccctcacc cttgggcaca ttcagagaac gctccaacac gtcgaatttt cccgctgcat
      301 acaccttgtc agaacaaata ccggtcggga acagtacgcg catccgatga tcctcggcgg
      361 tgttattaat cgtggttgtg aagtcgatcc gtcgcgaacc cgcgctcagt gatacaagcg
      421 tcttgacaga gactgcaacc ttggcagagg aacgttttcg tttcggcgtg acctctttcg
      481 gaatccgcag agtttgctcg atcccaagcg ttgctttgaa acccaggtct tccagaactt
      541 tcactgaaga tacagtgccg cacttggtcg aaagaaacgt ctcggttgct tcctgcggca
      601 atggggagta atcatactca tccccgacat cttcgatgtc ctcgatgaga tggagactgg
      661 gatagacctt acgggtagat ttatcttcga ccttcagtgt gccgttcttg ttgacgctca
      721 gtctgaaaaa ctcattttcc agacctgtct ttcgggcagt cacactcggc ttaggatcct
      781 tgcccctggc agcagtcacg tagaaagtct tcaaaccgaa tgagggaaga gcctctgcca
      841 taaactcggc atccacgtcc accacaagag acttctccaa cttgccatcg cgccaatgat
      901 gctgctcgcg aacgggatga accacagtct ttgcgtgcat tgttttgcct gatccatcaa
      961 ccaccgtcgc cttgccgtca gcaatcagtt cagcaggcac cgccgtccga atccgaacgg
     1021 gttcgttgcg ctgccaggtt gatggattga acgcaacaac cgctgctcct ttggggccat
     1081 ttgctgtgtt ggtattggca gtcagagcgc cgagcgcctg atcggtcaac gcctcagaca
     1141 cctgtcggga atgggcgaat cggctcacca tgtcccgatg caccgcatca atgctgcacc
     1201 cgcagatgtc atcatgcgga tgattcttca gcgtttcctt ccaggcatag gtcagaaatc
     1261 caccaggata ctgtccaccc aagacgaagt tcatggcact gagtggctcc gcgtatgctt
     1321 ccagaagcct gagacatcgc tcattggctt gcttgaggta cattcgggct gagaacacac
     1381 cggacagcaa cgggaagtaa cgtccattgt gcagttcccc ttgatgaggt ttgagtcgcg
     1441 gtttggcctt tttgagtgcc tcgacatact cttcatagtc accctgaatg agtttgcagt
     1501 tgtcgagttt ctcgttggtg tatttgatca tcgcgggaac cgatggctgc gcgggaagat
     1561 gatccactcc attgttgaga aggagaacgt tggttgtcgg acgatgtgcg atcaagtgat
     1621 tgatcgcatt tcgagcatgt tcaacggctt ggtcaaagtc cacgcgttca tcttccaagg
     1681 ccactccatg aatacgaccc caggaatgca gattgccata gcccccaatc agatgacagg
     1741 cgatgacggc agtttcccca tcgggagcaa tccaccgaaa ctccgtgttc aaattgtcac
     1801 cctcttcacc gataccacgc ataaagagca cggagtcaat gccaaagccc tttaggattt
     1861 ggggaagctg tgaaacgtgt ccaaaaggat ccggaatgta tccggccttc atgacacgcc
     1921 caaaacgttt ggcaacttga tgtccgataa gcagattacg caccaaggcc tctccgctca
     1981 caagaaactc atcgggaagc acataccaag gtcccacaga aagagccttg ctctttacat
     2041 ggcgagtcaa ggctttctca aactcaggcc tgacttgcag cacgtcctcc aagacaaccg
     2101 tctgaccatc gaacgtgaat cggtactttg gattcttctc caggatcttg agaagattct
     2161 ccgtcagacg aaccagtcgg atccgaaaat gttcgaagct gagataccag gctcgatccc
     2221 aatgggtctg cggcacaaca taggcaacgt acttccgatc tttcttactc atacatttct
     2281 cctgcaaaaa cgtttcattg ggtcttggca caacgtgtat tccgacacaa taccaccaag
     2341 acaatcgagc aggtaaagta ccaagcagaa aggcaaatgt caaaaacaca gactctcgat
     2401 caggagggag actcgtgctg aacatccaag acatggatca ccgtggcccc cagcagggtc
     2461 ttttggaagg acttctgaaa gcgcgcatcg gccgtgacca gcgggcagtc aagatgaaga
     2521 gccagagaca gatacagaca gtcgtaaaga cttgtatgga atcgaacgga gagatcatac
     2581 gcgccacgca gtaactccga tgacagatga aattgtactg gcagaaattc catctgccgg
     2641 agtatgttcg actcactctc gggaggtatg tccttcttgc gaaccttctt gctcagaatg
     2701 ttgtgcaact cgatagagag gaaatccgga gcatgcagag acgtctgctc acggacaagg
     2761 caagaagatt ctttcgcatg ttcctcaggg aatatccact tggcaacaac actggcgtca
     2821 acgacaaatc ggttcatcga ttgtctctgt cttcacgaat cagatcaacg ctgtccgtgg
     2881 tctgaggggg aagtgacgcc cgcagtcgcg ccagatcttc tcggatccta tctatgtcga
     2941 aaaccgtgga tgactcttcg aggatgcgtt tggcttccgc ctgcagagac cgtccattcg
     3001 cttttgctcg tttcttcagc cgtgccaccg ttttctcgtc caaatccctt accaacaatt
     3061 gtgccatggc ggccacctcc aaaatctccc acactgctat cataatgata gcagacaaag
     3121 agaaactgtc aaatccggag aaggcgttgt tttccccgct cagagaacgc cgcgctgctg
     3181 ccattcctgc atctttttaa ggatgggcat tgagaagtag aaactcaaac ttggaagcga
     3241 tgaggagaac tggttcttca actctccgat catctgctcg gcgtcatagc cgtccgccat
     3301 catcaagtgg cctcttcaag cctgactaac ctctccttgg actcacggcc gagcaaggga
     3361 acatagttct tgatgactgc ccagacaatc gcatcatcca cagtgtggta ctcgtgcgtc
     3421 aactgattcc gaaaatctac gatacgtcgc ccgcgcgtga tcagatcaaa cgtctcggga
     3481 gcaaaacgag agagtgccgc cattgcttcc ccaatgacaa tgaactcacg ttcgaccgca
     3541 gaccgcacga gacgcgagtt ttcatagcac gagaagtcga agcctgccac cgcgtcgttg
     3601 atagcctggc aggcttcgat gatatccaag agatacgccc tctcgtcacg cggcatagag
     3661 agtctctttc gttcgctcga tttcggcagc gatgtaagga ttcttgacgg ctccgtcaac
     3721 aacgagatcc acatccacgt tcaagagctc tctgagctcc tccaacatac cgaaatacgc
     3781 atccactctc gcacacgcat ccatcggctg gaactcgaca agcagatcca cgtcactctc
     3841 accaactcta aactgatccc ctgccgctga gccaaagaga tacagttttg agacaccatg
     3901 cctggcgcag atatcagata tctgttcttg tctgctttca agaaacctgg tcattgtcga
     3961 gttcctctcc ttatcatgtc ctgcaagtat gatagcaaag tgcttcggct atcgccaaac
     4021 cggcttctcc gacaacggca ctggtgacct gcgcccgtcc tgctttccag aatcatccgc
     4081 ttgccaaagg actctgtttt cttgagcgct caaagaacgc cacgctgctg ccattcctgc
     4141 atctttttga ggatgggcat tgagaagtag aaactcaaac ctggaagcga tgaggagaac
     4201 tggttcttca actctccgat catctgctcc gcgtcatagc cgtcggccat gagtttctcc
     4261 ttaagtgcgt caacgaccgg tggctcataa tacggactga tgcattccag gaagacgcta
     4321 agccgatgca tggccttctc cgatacgtct cccaccgtat gcgaaggagt ccagattcca
     4381 aaaacaacag gcttgttgca tttcgtcagt gcctccatgg gtgtgtggta gtcgaaccct
     4441 ggaaccaaac cgttgggaag atagagctgt gtgccaatga aatcgaaacc aacctccatc
     4501 ttcttttcca gtttcatcaa ctcgatatcc ggttgctcgt aggggttgaa cgccacgcca
     4561 cactgaaagc ggttcgggta ctcgcgccga atgtactgga gcagttcaac cgatgtggca
     4621 gcccccatcg gcgttggttc caaacccaac tcttctggct cgagccgatg cagtttcgga
     4681 ttgccatcgc cgctgataca caagagatac tgcaccccga gacgatcggc ggtttcaagc
     4741 atctgatcaa aatcctgttt gcggtgaaaa ctgttgagat gcacgacaac ccgctcacac
     4801 ggagcctcaa gaaactccag aacttctgtg gacgtgaaac gcaggttgcc catcggattg
     4861 tccggaagag agatggcaaa tccggcattg tagtaatcga agtagcgctt gccgaacttc
     4921 tcgaccgatc cctcgaaatc gctctgctcg agttcgggtg tgagaagctc cgcaagcaag
     4981 tgctcatgaa ggctcattgt gacttttcct cgtgatctgt tagtgtctgt tttgtctcag
     5041 tatcggatgg agttgtggtg tccggctcca tcgccgccag g
//
LOCUS       DAOQUC010000080         5056 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_18320_length_5056_cov_3.438512, whole genome shotgun sequence.
ACCESSION   DAOQUC010000080 DAOQUC010000000
VERSION     DAOQUC010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5056
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18320_length_5056_cov_3.438512"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1811
                     /locus_tag="PKH07_02280"
     CDS             <1..1811
                     /locus_tag="PKH07_02280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33804.1"
                     /translation="PDNFLSGIPADATLYWRAYGFNPDEVPGRTQESALYSFGRESIH
                     LVSPSNILSVTAPVVFQWTHGCPENRSWVVSLSLEPDFEETLLLSPKLDTTSWQIPQA
                     SWEVIPKGVPIYWAVLGYYQPTEWRVLTEWSHETWWFANNEAPRAVLDVSPSLQGAPP
                     LQVHFSGSATDPDGYLTDYYWIFDMTVTSEIMTVSSASVNDLQTRVYSSPGVYQVTFG
                     ATDNLQATDSVTRSVVVWTPTFTPTDTPTLTPSDTPTQTPTPTATDSPTTTPSHTHTE
                     TPTPTATETPTQTPTYTATASSTPTETLTPTALPVVFQATNANGPAGSTQELTVHLFN
                     APLVQRTVFMEIIYDPSVLQISADGIHCATDWLFPISLGRQNPLVDSHLGVISLTLER
                     DQIPLQRDIVPLLIVRCRINPAAQQDQFSLFRFATLIIDAANNVTIFGQHGVVRVSDA
                     PARPRWSLPDSISDSSATLRWFTADDSDLVGYHVLLVSTAGEVLSSTLTAPDTDTWTA
                     LALPSAARIFALLNALTQDQVSETEALQFETRIGPSTSKWDATRAFGAEWDPEGTSLP
                     ASGLSMLIPLTADINRDGRIDRQDLLEMLRIISSKD"
     gene            1875..3023
                     /locus_tag="PKH07_02285"
     CDS             1875..3023
                     /locus_tag="PKH07_02285"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNT33805.1"
                     /translation="MRRTLTIGNLWKAFQSPAVAASLLKGVAKAAIGYHLGNGYSLKP
                     GKVFCALTGCCNLKCVMCPQGNHPGFEGDLRQRSGGSLEDLIKLAAAIAPFRPFVAVS
                     GGEPFLHPHWREFVREIKRWGMICWIGTNGTMLERDAADLVEIGVDSISVSMDGPRDV
                     HDRIRGVAGTFDLVAKGIRELTRIRERTGRKKPEITAIFTITPHNSTHIPEMVEMVSS
                     LGVDVLRIGHLNFLTREEFESHSRLFQEVFKIDLDTSWEGFIASPDAIDSVSVAEAVA
                     KFRDQRNTPLRITFFPNFTPEETQQYYTSRRFRSTSFRNACMSAWDTAIVGPEMELIL
                     CPNYVLGSLRQESFERLWNCEKAVVFRRTLRRMKSFPACSRACCFFYM"
     gene            3048..4181
                     /locus_tag="PKH07_02290"
     CDS             3048..4181
                     /locus_tag="PKH07_02290"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNT33806.1"
                     /translation="MLWALAHETGNIGSRLREALRHSNATQRSNTLLNFAERSLGRAK
                     LLSLPSIVQVEVTNRCNLNCIFCSRHSHPLQLGDLPECLIPEISNFSARARETICFGY
                     GEPLIGKTFRGLIRDTRSGRISFTTNGLALTPALISELLAEVSRPLYNITFSIDALDE
                     ATYLSIREHSNFERVWENLRGLAALKASRDLTLPEIWINFVAMRRNIEQLPGLLISAA
                     EAGVSRILVFHLVVWDASYQAESLLGYPDLTKRIFDETRRIAGERGIRVDLPITISDG
                     GSRTPDDPEAMPLPKCHQPWSYVYIRHDGEVHACCFSERLKMGDLKENRFDEIWNNAA
                     YQNLRQTVNTSPPPDCLRCEMRFRYSRSPNERETYLKLTPRAK"
     gene            4186..>5056
                     /locus_tag="PKH07_02295"
     CDS             4186..>5056
                     /locus_tag="PKH07_02295"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNT33807.1"
                     /translation="MRIQEDERQAPTVDLVVGIVNWNTRNLLADCLQSVFRQTQGIQF
                     KVCVVDNGSTDGSAEMVQKCFPQVSLIRNESNFGFAQANNQILGCQQGRYALLLNSDT
                     RVIGNALKEMVDFMDTHPEAGAAGCQVLNEDGTLQKSCGRFPRLASIFFGGERCNQLF
                     RSLFGARRFFAEYGLSAEDHRQIQQADFVKGCSIILRRKTLEKVGLLDPDIFMYFEEI
                     DLCYRIKQEGNQVLYTPTAKIVHLGGGSCDCVPTTVFRNLDGQEFFFRKHYGQTQAIL
                     LRWTVAVGALLRLP"
BASE COUNT         1202 a         1317 c         1337 g         1200 t
ORIGIN      
        1 tcccggataa tttcctttca ggcattccgg cagacgccac actttactgg cgagcgtacg
       61 gcttcaaccc ggacgaggtt ccaggaagga cacaagagag tgctctgtac tcatttgggc
      121 gagagtccat tcaccttgtc tccccaagca atatactcag tgtaactgct ccggtcgtat
      181 ttcagtggac tcatgggtgt cccgagaata ggtcttgggt cgtgagcctg tcgctggaac
      241 ctgatttcga ggaaacgctt cttctctctc cgaaactgga caccacttcc tggcagattc
      301 cccaggcgtc gtgggaagta attccaaagg gtgttcccat ttactgggcc gtgttaggtt
      361 actatcaacc gactgaatgg cgggttctta cggaatggtc gcacgagaca tggtggttcg
      421 cgaacaatga agcaccacga gctgtattgg atgtttctcc ttcgctgcaa ggggctccac
      481 cgctgcaagt gcatttttcc ggaagcgcga cagatccgga tggctatctg actgattact
      541 actggatctt tgacatgaca gtgacctcag agatcatgac ggtatcttcg gcatcggtga
      601 acgacctgca aaccagagtc tacagcagtc cgggcgtcta ccaagtcacc tttggagcta
      661 ccgacaatct ccaggcaacc gattctgtga caagatctgt ggtcgtgtgg actcctacct
      721 ttaccccgac agataccccg actctgaccc cgagtgacac accaacgcag actccaaccc
      781 caacggctac agactcccca acaacaactc catcgcacac tcatacggag acacccacac
      841 ccacagcaac ggagactcca acccaaacac caacatacac agccacagca tcaagtacac
      901 caacagagac attgacaccc accgccttgc ctgttgtctt tcaggcaacg aatgccaacg
      961 gcccggctgg ttcaacgcag gaactcacag tccatctatt caacgccccg ctggttcaac
     1021 ggactgtttt catggaaatc atctatgatc cttccgtgct tcagataagc gctgatggca
     1081 tccactgtgc cactgattgg ttgttcccca tttctttggg aagacagaac ccccttgtgg
     1141 atagccatct tggtgtcatc agcctcacgc ttgaaaggga tcagattcct ctgcaaaggg
     1201 atatcgtccc cttgcttatt gtacgctgtc gtattaaccc tgcagcacag caggaccagt
     1261 tctctctttt ccgattcgcc accctgatca ttgatgcggc aaacaatgtg acgatttttg
     1321 gacagcatgg agtcgttcgt gtgagtgatg caccggcaag acctcgctgg tcattgcccg
     1381 actcaattag cgacagttcg gcgacgcttc gatggtttac ggcggatgac tctgatctgg
     1441 ttgggtatca cgtgctgctg gtttccacgg caggggaagt cctctcatcc actctcacag
     1501 cgcctgatac agacacatgg acggcactcg cgcttccgag cgcggcgcgg attttcgccc
     1561 tgctgaacgc gctcacccaa gaccaagtca gcgaaacaga agccctccaa ttcgagacgc
     1621 gaatcggtcc gagcacatcg aagtgggatg ccaccagagc tttcggagca gagtgggatc
     1681 cggagggcac gagtcttcct gcgtctggct tgagtatgct gattcctttg acagcagata
     1741 tcaaccggga tggtcggata gacaggcaag acttgctcga gatgctgagg atcatctctt
     1801 cgaaggactg agaaacagag gagattcgtt gacttcggga tctcaccccg agtcactgca
     1861 ctgccaggac gagaatgaga cggactctga cgataggaaa tctgtggaaa gcgtttcagt
     1921 cacccgcagt ggctgcgtca ttgctgaaag gagtggctaa ggcagccatc ggatatcatc
     1981 tcggcaacgg atatagtctc aaacccggca aggtgttttg tgcgctgaca ggttgctgca
     2041 acctcaagtg tgtgatgtgc ccgcagggca atcatccagg atttgaaggg gatttgaggc
     2101 agcgttctgg cgggagtctg gaggatctga tcaaactggc cgctgcgatc gctccattca
     2161 gaccttttgt tgcggtttct ggaggagagc cctttcttca tcctcattgg agggagtttg
     2221 ttcgggagat caaacgctgg ggaatgatct gttggatcgg caccaacggg acaatgcttg
     2281 agagagatgc agctgacctc gtggagattg gcgtggattc catctctgtg tccatggatg
     2341 gaccgcgtga cgttcacgac cgcattcgag gagtggcagg gacctttgat cttgtggcaa
     2401 aaggaatcag ggaactgaca agaatccgag aacgaacagg caggaagaag ccggagatta
     2461 ccgcgatctt caccataaca ccacacaatt ccacacacat tcctgagatg gttgagatgg
     2521 tctcttccct tggcgtggac gtcctccgca tcgggcacct gaactttctc acacgagagg
     2581 agtttgagag ccactccagg ctgttccaag aagttttcaa gatcgatctg gacacatcct
     2641 gggaaggatt catagcaagt ccggacgcca tagattccgt ttccgttgcg gaagcagtgg
     2701 cgaagtttcg ggatcagaga aataccccac tgcggatcac tttcttcccc aattttacac
     2761 ccgaggagac gcagcagtat tacaccagca ggagatttcg ttcgacctcg tttcgaaatg
     2821 cctgcatgtc ggcttgggac acggcgattg ttgggccaga gatggaactg attctctgtc
     2881 caaattacgt tcttggcagt cttcggcagg agagttttga gcgtctttgg aactgcgaaa
     2941 aggctgttgt cttcagaagg actcttcgac gaatgaagtc ttttccggca tgcagcagag
     3001 catgttgttt cttctatatg taaggattcg atcggaactt gagaaaggtg ttgtgggctt
     3061 tggcacatga aacgggtaac atagggtcac gccttcgcga agcgcttcgt cattcgaatg
     3121 ccactcagcg aagcaacacg ctactgaatt tcgcagaacg atcgctcggc agggcgaaac
     3181 ttctctctct accctcgatc gttcaagttg aggtaacgaa tagatgcaac ctgaactgca
     3241 tattctgttc ccggcactcc cacccgctgc aactgggtga tcttcctgag tgtttgatcc
     3301 cggagatatc gaacttctcg gcgcgagcca gggagactat ctgcttcggt tatggtgagc
     3361 ccttgatcgg caaaacgttc cgcggtctga tccgtgacac ccgaagcggc cggatttcct
     3421 ttaccacaaa tggacttgcc ttgacgccag ccttgatctc tgaactgctg gccgaggtca
     3481 gcagacctct gtacaatatc accttttcga tcgacgcttt ggatgaagcg acctatctct
     3541 ctattcgaga acactccaac tttgaaaggg tctgggagaa tcttcgagga cttgctgcgc
     3601 tgaaggcgag ccgagatctc accttacccg aaatctggat aaactttgtg gcaatgagac
     3661 gcaatatcga gcaactcccg ggactgctca tcagcgcagc agaggccgga gtctccagaa
     3721 tccttgtctt tcatcttgtt gtatgggatg cgtcctacca ggcagagagc ctcctgggct
     3781 atccggatct gaccaaacgc atatttgatg agaccagacg cattgcaggg gagagaggaa
     3841 tccgagtgga tctgccaatc acgatatcag atggcgggag tcgtactccg gacgacccag
     3901 aggcgatgcc acttcccaag tgtcatcaac cctggagcta tgtttacata cgacatgatg
     3961 gtgaggtcca tgcctgttgc ttttccgaga gattgaaaat gggtgatctg aaggaaaacc
     4021 gatttgatga gatctggaat aacgctgcat atcagaatct gcgtcagaca gtcaatactt
     4081 cgccgccccc tgactgtctc agatgcgaga tgcgctttcg gtatagtcgc agcccaaatg
     4141 agcgtgaaac gtatctcaag ttgacgcctc gcgccaagta ggccgatgag aatacaagag
     4201 gatgaaaggc aagcaccgac tgtggatttg gttgtaggca ttgtgaactg gaataccagg
     4261 aaccttctcg cagactgcct gcagtctgtc tttcggcaga cacagggcat tcagttcaag
     4321 gtctgtgtgg tggacaacgg ttcaaccgat ggcagtgccg aaatggttca gaaatgcttc
     4381 ccccaggttt ctctgatccg caacgaaagc aattttggat tcgcccaggc caataaccaa
     4441 atcctcggct gtcagcaggg gcgctacgca ctcctgctga acagtgatac tcgagtgatt
     4501 ggaaatgctc tgaaggaaat ggttgacttc atggacaccc atccggaggc aggcgcagca
     4561 ggttgtcagg ttctaaatga agacggcact ctgcagaaat cgtgtggaag atttccccgt
     4621 ttggcgtcca ttttcttcgg cggagaacgc tgcaatcagc tgttccgatc tcttttcggc
     4681 gcacggcgtt tctttgcaga atatggtctt agcgccgagg atcatcgcca gattcagcag
     4741 gcagatttcg tcaaggggtg ttcgatcatc ctgaggagga agactctgga aaaggtagga
     4801 ctcctcgatc cggatatctt catgtacttc gaggagatag acctttgcta tcgaatcaag
     4861 caagaaggga accaagtact atacaccccc actgcaaaga tcgttcactt gggcggtggc
     4921 agttgtgatt gtgtgccgac gaccgtcttt agaaatctgg atggacagga gtttttcttt
     4981 cgcaagcact atgggcagac tcaggcaata cttctgagat ggactgtggc cgttggtgca
     5041 ctacttcggt tgccat
//
LOCUS       DAOQUC010000081         5043 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_18378_length_5043_cov_2.481957, whole genome shotgun sequence.
ACCESSION   DAOQUC010000081 DAOQUC010000000
VERSION     DAOQUC010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5043
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18378_length_5043_cov_2.481957"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1360)
                     /locus_tag="PKH07_02300"
     CDS             complement(<1..1360)
                     /locus_tag="PKH07_02300"
                     /inference="COORDINATES: protein motif:HMM:NF028500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNT33808.1"
                     /translation="MSSASEQSERLVQELRFQAVLEELKKPVQYLKGVGPQRTSSLLE
                     LGIKSLNDLLFHIPRDFQDRSHIVPIADLEVANCQYVVGDVVSVNESLPKSQNVRHIL
                     KAIVTDGSGQLQVVWFNQRHLAHSIVPGATLALYGTVDLEKHQMQMLSPEWEIVVDEN
                     PESRTGIIPIYPLSQGLSQKALRAIILESLRRVRDDVPDWLPESVRSQRGLLRWSEAI
                     RMIHWPGGSMGMTSKTGFRLNFERARKRLSYDELFTTQMTLALNRITHMEKPGIAHEL
                     KYTVKNYTSQHCTSTQSLCHRFVEQLPFRLTKAQVRAMGEIEDDMASRRPMYRLLQGD
                     VGSGKTVVLLYGMLVAVENGKQAALMALTEVLAQQHYATLMNLSKGLGLSVALLYGGL
                     GLTDAKKTRAELAMGRVNLLIGTHALFQEKTEFSSLSMVVVDEQHRFGVGQRQAFQAK
                     GDQ"
     gene            complement(1382..>5043)
                     /locus_tag="PKH07_02305"
     CDS             complement(1382..>5043)
                     /locus_tag="PKH07_02305"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HNT33809.1"
                     /translation="PDILGNTTGVALLEDTTAGIAIGYHFEQGSGTPYDSKEFYLSIP
                     YGVTYSASMSKYRILEYKDLLLGKLIIDGTDEVPMDGESQCLRLQEIFWRDLRLLKVR
                     LEPSRIANYSSGKGGVLGEAVFRIVFSGQADTGDIPENPVSTPFETLYKMVLLNEEHS
                     LERRGRIPEFGREEAPTGYQKMPDGSPSMRFEISEQGIYRLTREELESHGLSWESCQN
                     ISLWKKGARIPCSVRRADSTQGLAEDGILFYAPTPGDEFNPRDSYWLGSDPLGMCLMD
                     SADPLPTSGKVLEVTSFRETLEFREQKAFPKEGHPSPRWYWQGISLSRGGTIRVPFSV
                     PGIDETQHVTSPISLTITLYNASGDDVKVQIKAPGDVQSDFSVARYSEHIASVTLDFS
                     TALLSEPFLTISAEASAVGLAEKGHLYLESVKVDYVRRLDLHNGRVLVKLDRVEPEIV
                     RRLIVRGLPDIGRHVEVFDIISSASPLCMTRGLTHSATQTYLDVDFRAHRILIQNANS
                     VPPPEDLRRDTPSNLRDSNVQCDYIFLSHHAFLNALEPLKAHRESQGLKVLLVDVQDV
                     YDEFSHGMKSIESIRLFLRHALTYWQSPIPAYVLLVGDANWDPWDRRGLGTPDYLPTY
                     LSERRPYTPAEDNWYFLVNGDSGFEHMMWGRLAARSEDDVRAVIEKIKTNDLNAPLGW
                     WRSKVLMVCDDGFEKEMEKSAESVADAGAICVRAFQTDYPYVTNMRLKDNGLKVKFCT
                     AATVEILKQINEGVLAYYYMGHGGANVMSAERIFLGGARGDSDVLKLENAPRFPFFFS
                     MSCLTGLYTFDDPAPGVSIAEDWQTRSNRGGISAYCPSGKGGTSDHMVLAECLNESLM
                     RDRLSTFGEVFAAAKLRFLHRRRSSMLNNQYIFFGDPASRPVWPSVPVAISPAMIARS
                     IHETTTEFVVCKIHDERLSRAKAELKLTTLSGEILWRHGPVSLTSGELAVNVPLKPGQ
                     EVDGQLSCYVWDDEQRVDAIGSVRVKVTLPNLRLSSLNVSTLSSEDGIVTDVKVENIS
                     TVPVIAPVTLLWRCNAMEERAIIPPLDVGAVHSVTAEFHILDALCDVDVILDPDELLQ
                     ESKREDNRISKKHFFSRADSSRKAEVRISQEDIRYQSKRIASSQQGVESVTGEIKTVL
                     WNPGGQDADGLRIILVDEDSGRTLYEERRVRVPAGGNLEVSAPCPFDVRNVRVDVFGP
                     VLSRPAVHKGKESSN"
BASE COUNT         1246 a         1383 c         1216 g         1198 t
ORIGIN      
        1 cttggtcccc ttttgcctga aatgcctgcc tttgtcctac accaaatctg tgttgctcat
       61 caacaacaac catactcagt gaggagaact cggttttctc ctgaaagagt gcgtgtgttc
      121 caatcaggag atttacacga cccatggcaa gttcagcccg agtcttcttg gcgtctgtca
      181 gccccagacc accgtacaat aaggcaacag acagaccgag acccttcgag agattcatca
      241 aggttgcata gtgctgttgg gcaagcactt cagttagtgc catgagtgct gcttgcttac
      301 cgttttcaac agcaaccagc attccgtaga gaaggactac agtctttcca gaacctacgt
      361 cgccctgaag caagcgatac attggacgtc gagaagccat gtcgtcttcg atctcgccca
      421 tagcccgtac ttgtgccttc gtaagcctga atggcagttg ctcaacaaac ctgtgacaga
      481 gactctgagt tgacgtgcag tgctgagacg tatagttctt aacagtatat ttcagctcgt
      541 gtgctatccc aggcttttcc atatgtgtga tgcggttcaa ggccagagtc atttgagtcg
      601 tgaacaactc atcataggaa aggcgttttc ttgcccgttc gaagttcaga cggaagcctg
      661 tcttggatgt catccccata ctgccgcctg gccaatggat catccgaatc gcttcagacc
      721 agcgaagcag acccctctgt gagcgcaccg actctggtaa ccaatcgggg acatcgtccc
      781 gaacacgtct caatgactcc aggatgattg ccctgagtgc tttctggctg agaccttggc
      841 tcaaaggata gatggggatg attcctgtcc ttgactcggg attctcatca acaacgatct
      901 cccactccgg actgagcatc tgcatctggt gcttttccaa atccacggtg ccatagagtg
      961 caagcgtggc accaggaacg atggaatggg ctagatgtct ttgattgaac cagacgacct
     1021 gtagctgtcc gctaccgtca gtcacgatgg cctttagaat atgtcgcaca ttctgtgact
     1081 tgggaaggct ctcgttgacg gagacaacat cccctacaac atattggcag ttcgcaacct
     1141 ctaggtcagc aattggaacg atgtggcttc ggtcttgaaa gtcacgcgga atatgaaaga
     1201 gtagatcatt cagactcttg atgcccagtt ccaagaggga tgaagttctt tgggggccaa
     1261 cacccttgag atactgaacc ggcttcttca gttcctccaa gacggcttgg aatctcagct
     1321 cttggacaag cctctcggat tgctctgagg cggatgacaa agaagaatcc tcacgcatcc
     1381 cctagttcga tgattccttt cccttgtgca ccgcgggacg gctcagaacg ggcccgaaaa
     1441 catcaacgcg cacattccga acgtcaaacg gacaaggagc cgacacctcg agatttcctc
     1501 ccgctggaac tcgcacacgt ctttcctcgt aaagcgtccg ccccgagtct tcatccacca
     1561 agatgattcg cagaccgtca gcgtcctgac cgcctggatt ccacaagacc gtcttgatct
     1621 cacctgttac cgattcaact ccctgttgcg atgaagcaat gcgtttgctc tgatatcgaa
     1681 tatcctcctg cgatatccga acttccgcct ttctcgacga gtcggctcga gagaaaaaat
     1741 gcttcttcga gatccggtta tcctcacgct ttgattcctg aagaagttcg tccggatcca
     1801 gtatcacatc aacatcacac aaggcatcca agatatggaa ctccgctgtc actgaatgaa
     1861 ccgccccaac atcgagaggc ggaatgatcg ctctctcttc catagcattg catctccaca
     1921 gcagagtgac cggagcgatg actggaacgg tgctgatatt ctcaaccttc acatcagtga
     1981 caattccatc ttcagaagac agagtgctca cattcagaga agacagacga aggtttggaa
     2041 gcgtcacctt cacacgaaca gagccaatcg cgtctaccct ttgttcgtca tcccaaacat
     2101 aacagctgag ctgtccgtca acctcctgac ctggtttaag cggaacgttg acggccaatt
     2161 caccactcgt cagcgaaaca ggtccgtgtc gccagagtat ctcccctgaa agagtcgtga
     2221 gcttgagctc cgctttggcg cggctcagtc tctcgtcatg gattttgcat acaacgaact
     2281 ccgttgtcgt ctcatgaatg cttctggcaa tcatcgcagg agagatcgct actggaacag
     2341 acggccatac aggtcggctc gccggatctc cgaaaaaaat gtactgattg ttgagcattg
     2401 atgaacgacg acgatgcaga aaacgcaatt tggccgcggc aaaaacttca ccgaaagtgg
     2461 aaagacggtc ccgcatcagg gactcgttga ggcactcagc gagaaccatg tgatcggatg
     2521 tccccccctt gcccgaggga caataggctg agatgccacc ccgattggag cgagtctgcc
     2581 agtcctcggc aatggaaact cccggtgccg gatcgtcaaa tgtatagagt ccagtcaggc
     2641 aggacatgct gaaaaagaac ggaaatctcg gcgcgttctc aagcttcaga acatcgctgt
     2701 ctccccgcgc cccaccgaga aagatccgct cagcgctcat cacattcgcg ccaccgtgtc
     2761 ccatgtagta gtaagcaagg accccctcgt ttatctgctt gagtatttca actgttgctg
     2821 ccgtgcagaa cttcaccttc aaaccgttgt ctttaagtcg catattggtg acgtatggat
     2881 agtcagtttg aaatgcccgg acacaaatcg ctcctgcatc agcaacactc tcagcagatt
     2941 tctccatctc cttctcgaaa ccgtcatcgc acaccatgag cactttggaa cgccaccatc
     3001 caagaggggc attcaaatcg ttggtcttga tcttctcgat gactgcccgt acatcgtctt
     3061 ctgaacgagc tgccagacgt ccccacatca tgtgctcgaa acccgagtct ccattgacca
     3121 gaaagtacca gttgtcttcg gctggggtat acggacgtct ttcactcaag taagtcggga
     3181 gatagtccgg agtacccagt ccacggcgat cccagggatc ccagtttgcg tcaccgacga
     3241 gaagtacata ggctggaatg ggactctgcc agtaggtgag tgcatgtcga aggaagagtc
     3301 ttatagattc aatggacttc atcccgtgcg agaattcgtc atacacgtcc tgaacgtcaa
     3361 caagaagcac tttgagtcct tgggattcac gatgagcctt gagcggttcc aaagcgttta
     3421 gaaacgcgtg atgagaaaga aaaatgtaat cacactgaac gttgctgtcc ctgagatttg
     3481 aaggtgtgtc ccgtcttaag tcttcgggcg gcggaaccga atttgcattc tgaatcagga
     3541 ttcgatgcgc acggaaatca acgtccagat atgtctgggt tgcggaatgt gtcaaacccc
     3601 tagtcatgca gagtggagat gctgacgaga taatatcaaa gacctcgaca tgtcttccaa
     3661 tgtcaggcag cccccgaacg ataagtctgc gaacgatctc gggttcgact ctgtctaact
     3721 tcacgagcac tcttccattg tgcagatcaa gtctgcgaac gtagtccacc ttgaccgact
     3781 cgagatagag atgccccttc tctgccagac cgacagcaga tgcttccgca gatattgtca
     3841 ggaaaggttc cgaaagcaac gctgtgctga agtctagagt aaccgatgca atgtgttccg
     3901 agtagcgggc aaccgaaaag tcactctgaa catctccggg tgccttgatt tggaccttaa
     3961 catcatctcc cgaagcattg tacagtgtga tcgtgagcga tattggtgac gtgacgtgct
     4021 gtgtctcgtc aatacccggg actgagaaag gaactcgaat tgtcccaccg cgtgacaagg
     4081 aaatgccctg ccaataccac cgtggcgaag gatgcccttc cttaggaaag gccttttgtt
     4141 ctcgaaattc aagtgtctcg cgaaaagagg tcacctcaag gactttcccc gatgtgggca
     4201 gaggatcagc actatccatc aaacacattc caagtggatc agatcccagc cagtaggagt
     4261 ccctgggatt gaactcatcg ccaggcgttg gggcgtagaa caaaatgccg tcctccgcaa
     4321 gtccctgggt gctgtcagct cgacgaactg aacaagggat tctggcacct ttcttccaaa
     4381 ggctgatgtt ctgacacgac tcccatgata gtccatggga ctcaagctct tctcgggtca
     4441 gtctgtagat tccctgttcc gatatctcaa agcgcatact tggggagcca tccggcatct
     4501 tctggtagcc tgtcggtgct tcttctctgc caaactctgg aatccgacct cggcgttcaa
     4561 gtgaatgctc ttcgttaagg agaaccattt tgtagagagt ctcgaacgga gtgctgaccg
     4621 ggttctcagg aatatcgcca gtatctgcct gccctgaaaa tactattctg aaaacagcct
     4681 ctcccaaaac tccgcccttc ccagaggagt agttcgcgat tcgggatggc tctagacgaa
     4741 ccttcagcaa gcgcaagtca cgccagaata tctcctgcag tctgagacac tggctctcac
     4801 catccattgg cacctcatcg gttccatcaa tgatgagctt ccccaggaga agatctttgt
     4861 actccagaat ccggtatttc gacatgcttg ccgaataagt taccccgtag ggaatgctga
     4921 gatagaactc tttcgaatca tagggagttc cagatccctg ctcaaaatgg taaccgattg
     4981 caatccctgc ggtcgtatcc tcgaggagcg caacaccagt ggtgttccca aggatatcgg
     5041 ggc
//
LOCUS       DAOQUC010000082         5033 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_18419_length_5033_cov_3.433507, whole genome shotgun sequence.
ACCESSION   DAOQUC010000082 DAOQUC010000000
VERSION     DAOQUC010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5033
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18419_length_5033_cov_3.433507"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..663
                     /gene="araD"
                     /locus_tag="PKH07_02310"
     CDS             1..663
                     /gene="araD"
                     /locus_tag="PKH07_02310"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase AraD"
                     /protein_id="HNT33810.1"
                     /translation="MVHAGLVTLTWGNVSGADRDQGVMAIKPSGVPYDRLQPEDIVVL
                     SIKSGEVLEGSLSPSSDTPTHLVLYRVFPGIGAVVHTHSKYATGWAQAGLEIPCLGTT
                     HADHFRGSVPVTRPLRNDEVENDYEKNTGMVIFERFRDSEINPNEIPAVLVNAHGPFV
                     WGKSLDSAIENAITLEYVAECALLSTALNPNAIPISKVLLKKHFGRKNGKDAYYGQNP
                     KP"
     gene            complement(695..3100)
                     /locus_tag="PKH07_02315"
     CDS             complement(695..3100)
                     /locus_tag="PKH07_02315"
                     /inference="COORDINATES: protein motif:HMM:NF025125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNT33811.1"
                     /translation="MSHEKRTKTAALGYPVLAGLFLLLGLTVAISRVDALEGWSEVFT
                     LTPGARDIEDAVIAAQDQTIFVVYRKQGLFCICSEDNGKHWSEPVLASGDAKGSRFPS
                     ILFKDSVLHLAWSAPMTVATEVVNQIFYTQSSDQGRTWRAPIHLAETTTHGMRPQILP
                     IRGGIVIIWYELDQRQVLSKSPLDFKLIERYLENPALNVIPIEKRDVVRSSIQAIRSL
                     NEGKSFLGARKTVHETLGALTVFSAYETADHRIGVCWDDKQSVLFMESNDDGATWDYN
                     WKLRDSIPTRTMLDQVLSTEGEIVQVLTPIEPFQNVSISVKVGERKPIEVVSMVKLQS
                     FPRIAQAGQDIHLAWVQQRDDEMVAKYMRTDKHRPTSRILSPTDPKITTPRFNIEWEG
                     QDDISSKMFFTHSLDKLNWANWESELYGITITTPEDGEYDFYLKAKDMAGNIQAEPAH
                     FHFNTFSVAPDTFLVGTPPKVVNSRSLSLEWDGFDNNPSVGSLQFQYRLDFGEWSTFS
                     QQQQVTFRDLSEGSHTVEVRAKDKSDNVDATPASHSFVVKLNIRPKFITVPEGLLSSN
                     TVSFHWEAQDDTTDKAQFFFSYQLDGGLWSAYSPASNLDLADLSEGRHRLLVRARDEQ
                     GNVSQETLEHEFVVDMTPPKVTIALSKLDSSTEHKPMIELQAEDNFSTGDDIQFQVRI
                     EEGTWTEWDPVRVVTLGTPIKPWSKGYRIEAKAMDEAGNITSEPEVYSLLLRDRYPLW
                     LLIVPIAVPAVIGLLVLIGFIKSSLASRRRRKSPKSTTPDDMSNFGQPPSASASTSED
                     DLF"
     gene            complement(3194..4198)
                     /gene="holA"
                     /locus_tag="PKH07_02320"
     CDS             complement(3194..4198)
                     /gene="holA"
                     /locus_tag="PKH07_02320"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HNT33812.1"
                     /translation="MDFLKFKPLSSEGRVRPFYHLTGTEGYHVRTALDRIEEAVRKNV
                     SASAERENYSAKDRWAEAISSFCTVPLFGDVKIVVVTDLEALAKTASTADKVLAALTS
                     LVKSPPDNAFLVLCSSEMDGRTKLAKFLSKELCEVNCSPLKPYQFEQWVAERVQKANL
                     NLSPKALDSLVVRTNGSMEGILNEIEKLALFATPGKPISESELERLVADSAEEPIYLL
                     YDALDSADRERSFRMLKDVLSHLDSPLQVVFGLSKHIRMLLGLLALLDQGKRDDEIRS
                     SLGLSSFQIGNLRDKIPNLKRLPLRRMLQLLELADERIKTSSQSGEELLYQLVGCLST
                     "
     gene            complement(4288..4776)
                     /locus_tag="PKH07_02325"
     CDS             complement(4288..4776)
                     /locus_tag="PKH07_02325"
                     /inference="COORDINATES: protein motif:HMM:NF016290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptE family protein"
                     /protein_id="HNT33813.1"
                     /translation="MKRTIAVVMLLAGVAGGCFTLGERVLPESIQTVYIPYIKNQTME
                     YGVEEQVTDTLIREFVKDGRLKVVSHPEDADSVLEGHISKYDISPGERNAAGRTISHF
                     VDVSVVLTMRDLRTGEVIMEPTTFSEGGVYFLSTLPGRDRQTLILARLCEDVISRVIE
                     GW"
BASE COUNT         1196 a         1351 c         1308 g         1178 t
ORIGIN      
        1 atagtccatg caggtttggt taccctaact tggggaaatg tgagcggcgc cgatcgcgac
       61 cagggagtca tggcgatcaa accgagcggg gttccctatg accggctcca acccgaggac
      121 atcgtcgttt tgtccatcaa gagtggagag gtgcttgagg gatctttgag tccgtcgtcg
      181 gatacaccga cccatctggt gctgtaccgc gtatttccag gcatcggtgc agtggtccat
      241 acccactcca agtacgcgac cggttgggca caggcaggac tggagattcc ttgcctgggc
      301 acaacacatg ccgaccattt tcgcgggtct gttccagtca ctcgtccgct gcgcaatgat
      361 gaggtcgaga atgactacga gaagaacacc ggaatggtca ttttcgaacg gtttcgagat
      421 tccgaaatca acccgaacga gattcccgca gttcttgtca acgcccatgg tccttttgtc
      481 tggggaaaat ctctggactc ggccatcgag aacgcaatta ccctggagta cgttgctgaa
      541 tgtgccttgc tgtccacggc tctcaatccc aacgccattc ccatctccaa agtgcttctc
      601 aagaagcact tcgggcgcaa gaatggcaag gacgcctatt acggacagaa ccccaaacca
      661 tgatgatgtc gtgcgtcagg cagatacctt cggtctagaa cagatcatcc tcgctcgttg
      721 aagcagaagc cgagggaggc tgaccgaagt tggacatgtc atcgggtgtg gtggacttgg
      781 gggactttct tcgtctcctc gatgccagac tgctcttgat aaatccaatg agaacaagca
      841 ggccgatgac agcaggaacg gcaattggca caatgagcag ccacagggga tatctgtcac
      901 gtagcaggag gctgtacacc tcgggttctg aagtgatatt gcccgcctca tccatcgcct
      961 ttgcctcgat cctgtaccct ttgctccaag gtttgattgg cgttccgagc gtcaccacgc
     1021 gaacaggatc ccattccgtc caagttccct cttcaatgcg tacttgaaac tggatgtcat
     1081 cgccagtgct gaaattgtcc tccgcctgca attcgatcat cggtttgtgt tcggtggaag
     1141 aatccagttt tgaaagtgcg atggtcacct ttggcggagt catatccacg acaaactcgt
     1201 gctcgagtgt ttcctgcgag acgtttccct gttcgtcgcg tgctcgtacc aagagccgat
     1261 ggcgaccttc agagagatcg gccagatcca gattggacgc tggagagtac gctgaccaga
     1321 gaccgccatc caactggtag gaaaagaaga actgtgcctt gtccgtcgtg tcatcttggg
     1381 cctcccagtg gaacgacacg gtattgctgg aaagcaagcc ttcgggaacg gtgatgaact
     1441 tgggacggat gttcagcttg acgacaaaac tatgactggc aggagtggca tccacattgt
     1501 ccgacttgtc cttggcacgg acttcgacgg tatgggaacc ctcgcttaag tctcggaagg
     1561 tgacctgctg ttgctgggag aaagtagacc actcaccgaa atccaatcgg tactgaaact
     1621 gcagggagcc caccgaagga ttgttgtcaa aaccatccca ttccagcgag agagagcgag
     1681 agttgacgac cttgggggga gtgcccacga gaaatgtgtc aggagcgact gagaatgtgt
     1741 tgaaatgaaa atgagcgggc tcggcttgaa tgttgcctgc catgtccttg gctttgagat
     1801 agaaatcgta ttctccatct tccggtgtgg tgatggtaat cccatacagc tcggactccc
     1861 agtttgccca gttgagtttg tccaggctgt gggtaaagaa catctttgag gaaatgtcat
     1921 cctgaccttc ccattcaatg ttgaatcggg gcgtcgtgat ctttggatca gtcggtgaga
     1981 ggatccgtga agtggggcga tgcttgtccg tgcgcatata cttggccacc atctcatcgt
     2041 cccgctgctg aacccacgca agatgaatgt cttggcctgc ctgcgcaatt cgaggaaagc
     2101 tctggagttt aaccatggag acaacttcaa tgggcttgcg ttctcccact ttgacgctga
     2161 ttgaaacatt ctgaaatggc tcaatgggag tgagaacttg aacaatctct ccttcggtgc
     2221 tcagcacctg atctaacatg gttctggtgg gaatcgaatc tctgagtttc cagttgtagt
     2281 cccatgtagc gccatcgtca ttgctctcca tgaagagcac ggactgcttg tcatcccagc
     2341 agactccgat cctgtgatcc gccgtttcat aagcagagaa gacagtgagt gccccaagtg
     2401 tttcatgaac ggttttccgt gctccaagga aggatttccc ctcattgaga gaacggatcg
     2461 cctgaatgga tgaacggacg acatctcttt tctcaatagg aatgacattc aacgcagggt
     2521 tttcgaggta gcgctcaatc agtttgaagt ccaaaggaga cttggagagc acttggcgct
     2581 gatccaattc gtaccagata atcacaatgc cccctcggat tgggagaatc tggggacgca
     2641 tcccatgcgt ggttgtctcg gcaaggtgaa tgggtgcccg ccacgtccgt ccttgatccg
     2701 aactttgcgt gtagaaaatc tggttgacga cttccgtcgc aaccgtcatc ggagcagacc
     2761 acgccagatg aagcaccgag tccttgaaaa gaatggacgg gaaccgggag cctttggcat
     2821 ctcccgatgc cagaaccggc tcactccagt gcttgccgtt gtcctcggaa cagatgcaga
     2881 aaagaccttg tttacgatag acaacaaaga tcgtctgatc ttgcgcagcg attacggcgt
     2941 cttcgatatc ccgtgctcca ggcgtcaggg taaaaacctc actccatccc tcaagagcgt
     3001 caacccgtga gatagcaaca gtcagtccca gcagcaggaa tagccccgcc aggacgggat
     3061 accccagagc ggctgttttt gttctctttt cgtgcgacat tgcctcacac acgccttgtc
     3121 tgtctccttc tattcattat cgttctccgg atgatctttc ttgatgtttt ccggaaaagt
     3181 caggtcggtt ccctcaagtc gagaggcacc ccaccaactg atacaacagt tcctcacccg
     3241 actgcgaact tgtcttgatc cgttcgtccg caagttccag caattgaagc atacggcgga
     3301 gaggcagccg tttcaagttt ggaattttgt ccctcaagtt cccgatctgg aagctggaaa
     3361 gacccagact gcttctgatc tcatcgtccc gcttgccttg atctaaaagt gccaacaaac
     3421 ccaagagcat ccgaatgtgc ttcgaaagcc cgaaaaccac ttgtagaggg ctgtccaggt
     3481 gcgagagcac atccttgagc atacgaaaag agcgttccct gtccgccgaa tccaaggcat
     3541 cgtagagcag gtagatcggc tcctcggcgc tgtcggccac aagccgttca agttcggatt
     3601 cggagatcgg ttttcctggg gtggcaaaca acgccagttt ctcgatctcg ttcaggattc
     3661 cctccatgga gccattggtt cgaacgacaa gactgtccaa ggccttcgga ctgagattga
     3721 gatttgcttt ctgcacacgt tcagccaccc attgctcaaa ctgatagggc ttgagtggcg
     3781 aacagttgac ttcacagagt tcctttgaga gaaacttagc cagttttgtc cgaccgtcca
     3841 tctcggaaga acacaacacc agaaaagcgt tgtcgggtgg gctcttgacg agagaagtca
     3901 gtgccgccaa aactttgtcg gcagtcgaag cggtcttggc aagtgcctcc agatctgtca
     3961 caacgacgat cttcacatca ccaaacaggg gtacagtaca aaaagagctg atcgcttccg
     4021 cccagcgatc cttggcggag tagttctcgc gttccgcaga agcggagacg ttctttcgaa
     4081 ccgcctcttc aatccggtcg agagccgtcc tgacatgata cccttccgtg ccggtgaggt
     4141 ggtagaatgg acgaacacgc ccttctgaag aaagaggctt aaacttcaag aaatccatgc
     4201 gtcaccactt cagcaatgtc aatgtcacta catgaatcaa atgcatttag gaaagggtac
     4261 aggtaccttt cgttgtttgc actctggcta ccaaccttca atgacccggc tgatgacgtc
     4321 ttcgcagagc cgagcgagaa tcagagtctg gcggtcacgg ccaggaagag tggatagaaa
     4381 gtagactccc ccctcggaga aggtcgtcgg ttccatgatg acttcgcccg ttctcaggtc
     4441 gcgcatcgtc agaacaaccg acacgtccac gaagtgggat atcgtacgtc cagcggcatt
     4501 tcgttcgcct ggactgatat cgtatttgga gatatggcct tccaagaccg agtcggcgtc
     4561 ctcagggtgg gagacaacct tgagccgacc gtctttgaca aactccctga tcagggtatc
     4621 ggtcacctgc tcttccaccc catactccat cgtctgattc ttgatgtatg gaatgtagac
     4681 ggtctgaatg ctttcgggca gaactcgttc acccagagtg aaacagccgc cagcaactcc
     4741 cgccagaagc attactacag caatggttct cttcatgatt caaccctctc gtcgccccaa
     4801 acagttttct tgggagtcga aacacccatt ctcaatccga tcctgtagtc ggtgcgccag
     4861 tgcagccgat ccaaactctg ccgccttctc tctgcgtctt gtgaaaagga aagccggtgc
     4921 ttccctgcgc aggagctaca atgcccccga gacggcgagc acagacgcac tctcaagata
     4981 gaataagact accacagaac aaaccgaagg ggaatagata tgaataaaat cag
//
LOCUS       DAOQUC010000083         5019 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_18497_length_5019_cov_3.178485, whole genome shotgun sequence.
ACCESSION   DAOQUC010000083 DAOQUC010000000
VERSION     DAOQUC010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5019
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18497_length_5019_cov_3.178485"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            371..1630
                     /locus_tag="PKH07_02330"
     CDS             371..1630
                     /locus_tag="PKH07_02330"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNT33814.1"
                     /translation="MQQNQDGKMNRRDFTKVSAAAGFGILAGNAFAGKKNVDTLKVGL
                     LGCGGRGTGATVNMLEGNENVLLVAMADLYQDRLDNARDKMKNHENAAVAQKYAVQDD
                     HCFVGLDAYWQILNTDIDILIEGTPPYRRPKHIERAVLCRKHVFMEKPVAVDPEGIRK
                     IMEASERARTLGLSIVAGTQRRHQQDYIETIKRIQDGKIGDVLALRCYWCSGLPWARE
                     RQEGWSDLEHRTRNWLNNCWTSGDSIVEQHVHNLDVCNWVMGTHPIRVLAVGGRTWKP
                     PEEKYGDNFDHFACDFEYENGVHLLSVCRHWNGCTDRVSEAVVGSKRESNCVDMARKR
                     ENSVNPYVQEHVDLVKSIRGEGPYLNEGVQVAESTMTAIMGRMAAYTGQMQVWNKALQ
                     TDLGLVPDDLDFAKEYPCGPVPCPVKL"
     gene            1647..2531
                     /locus_tag="PKH07_02335"
     CDS             1647..2531
                     /locus_tag="PKH07_02335"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HNT33815.1"
                     /translation="MDRRAFMAACGTVAGGLVLMGESSSHGEAVEGAFSKLPIKISAP
                     LGWFKGGTNEEKLEEVAKWGLPAYEWLGPSGDLDSLRAKADSLGIELSCIVGAGAIAP
                     GHMVQPEQHDQVVAQFQERVKIAKQLNCKQLIGLTGNERDDVSRDQQTEYVIACLKRL
                     APIAEENDVVLVMETLNPLVDHRGFFLNRTDHAVQIMQAVNSPNVKICFDIYHQQITE
                     GNVIRNIRENIQWIGHFHVADNPGRKEPGTGELNYRNIFKAIAETGYSRFVALECGHS
                     TGNYEDTLRRTLECLSWT"
     gene            complement(2599..3501)
                     /locus_tag="PKH07_02340"
     CDS             complement(2599..3501)
                     /locus_tag="PKH07_02340"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HNT33816.1"
                     /translation="MIAVTQDEIEGKAIFPLMAKGDLPYPPSQCVYGNDQVGVWLYQA
                     NCIEFMEMLIGQNPDGCFDMIFADPPYFLSNGGITCHAGKMVQVDKGQWDRSSGPELN
                     HEFNTTWLSRCQKLLKQNGTIWVAGTHHVIHSVGYAMQQLGMKILNNITWEKPNPPPN
                     LSCRYFTHSTETIIWASKNQDAKHCFNYDKMREANNGKQMKSVWTLTAPNKDEKGFGK
                     HPTQKPVSLLERIILASTSRGDLVFDPFSGSSTTGVAAVNTCRQFVGTELESEYISLS
                     VERLQHAIDIRQSSQGSANQSHLL"
     gene            3738..>5019
                     /locus_tag="PKH07_02345"
     CDS             3738..>5019
                     /locus_tag="PKH07_02345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HNT33817.1"
                     /translation="MRISRILLSLFVFSQVAFAGNFVRISPSGKYFQLEDGTSFVPIG
                     SNEAMNWPEMMCIYPNHGEYNPPGAEHYFQMLRKHGVNCIRIMAEAANTGIRNGGAFL
                     EEPVGEYVQDTVAFLDGLFELATQHHIYVILTPFDTFWMDKKWDQHPYNAVNGGPAHS
                     REEFLTSEAVFKCQQERMRSLIDRFGNSDFLFAWEILNEVDMRWGATPEQQRDWTARM
                     AKFVREYEKTKWGTNHLITVSLTETEVEADSRIPSLLLDNPFLDFVTTHIYYPQGRAP
                     VDPIQPALEFGRGIRRQLEQIEQSVQRPFFASEFGPVDLWPLPDELDDELFHNMSWAH
                     LATGAAGAGLRWPYRWHHILSEGMRESQLGLSLVASKIPWSTFAARNVDERLSVSTPD
                     GQKVHPVGCGDEQQFLIWLVRESNSKKVGDGDTQL"
BASE COUNT         1215 a         1253 c         1393 g         1158 t
ORIGIN      
        1 attctcctga aacctccaga tctgaagaaa aacctcacac cgaggatctg agacgggtgc
       61 atattacagg aaaagtgaca accggttcaa actattctga gcaggcctgc gcctgctggc
      121 atgtgggcta tgcccatcct cacataatgc ttgtccctct tagagaccac ttccttctaa
      181 gtcagcctta ccaagaacag gccagcgcct gctgaaagga ataggaagaa acacgatcca
      241 aagtcaagat caaaatcccg tcgcaatgca tccgcgcgga tttggattga caggcgtgct
      301 ccagcctgac aagctccttg atggtgttga cgggcgcggg taagaagaat ctttttgtgt
      361 ggagagagtc atgcaacaaa atcaggatgg caagatgaac cgacgggact tcaccaaggt
      421 atccgcagca gcgggcttcg ggattctggc tggcaacgcc ttcgctggaa agaagaatgt
      481 ggacacgctc aaggtcggcc ttctgggctg tggtggtcga ggcaccggag ccacggtcaa
      541 catgctggaa ggcaatgaga atgttcttct cgtggcgatg gcggatttgt accaggacag
      601 actggacaat gcccgtgaca agatgaagaa tcacgagaat gctgcggttg cccagaagta
      661 cgccgtacag gatgaccact gcttcgttgg cctggatgcc tactggcaga ttctcaatac
      721 tgatattgat atcctaatcg aaggcactcc gccctatcgc agacccaagc atatcgagag
      781 ggctgtcttg tgccgcaagc atgtcttcat ggaaaaaccc gtggcggttg atcccgaagg
      841 gattcgaaag atcatggaag cctcagagcg cgccagaacg ctcgggctgt ccatcgtggc
      901 aggcacacag cggcgtcatc agcaggacta cattgagacc atcaagcgca ttcaggacgg
      961 caagatcggc gatgtgctgg cgcttcgctg ctactggtgc agcggtcttc cctgggcgcg
     1021 ggagcgtcag gaaggttgga gcgatctgga gcatcgcact cgcaactggc tgaacaactg
     1081 ctggacgagc ggcgacagca ttgtcgagca gcatgtacac aacctggacg tgtgcaactg
     1141 ggtcatggga acgcatccaa ttcgagtgct ggcagtcggg ggacgaacct ggaagcctcc
     1201 ggaggagaaa tacggcgaca actttgatca ctttgcatgt gattttgaat acgagaatgg
     1261 cgtccatctc ttgagtgtct gccgtcactg gaacggctgc accgacaggg tcagcgaggc
     1321 ggttgtcggc agcaaacgtg agagcaattg cgtggacatg gcgcgcaaga gagaaaacag
     1381 tgtcaacccc tacgttcagg agcatgtgga tttggtcaag agcatccgtg gcgaaggccc
     1441 ttacctcaac gaaggcgtcc aagtcgcgga atccacgatg accgcaatca tgggacgtat
     1501 ggcggcgtac accggccaga tgcaagtctg gaacaaggcg ctacaaaccg atctgggact
     1561 ggttcccgac gatctggatt tcgccaaaga gtatccctgc gggccggtgc cgtgtccggt
     1621 aaagttgtag aaggagggtt tagagaatgg atcgtcgcgc attcatggca gcatgtggaa
     1681 cagtggcagg aggccttgtc ctgatgggag aatcatcatc gcatggcgag gcagtcgagg
     1741 gagccttctc aaaactgccg atcaagattt ccgcaccgct gggttggttc aagggtggca
     1801 caaacgagga aaagctggaa gaggtcgcca agtggggact gcccgcctac gagtggctcg
     1861 gccccagtgg agaccttgat tcccttcgag ccaaggccga ttctctcgga atcgaactca
     1921 gctgcatcgt cggcgctggc gcgatcgcgc ccgggcacat ggttcagcca gaacaacacg
     1981 atcaagtcgt ggctcagttc caagagcggg tgaagatcgc caagcaactc aactgcaaac
     2041 agctgatcgg tttgacagga aacgaacgcg atgatgtgtc ccgagaccaa cagaccgaat
     2101 acgtgattgc ctgcttgaaa cgccttgccc ccattgcgga agagaacgat gtcgtgttgg
     2161 tcatggagac tctcaatcca ttggtggatc acagaggatt cttcctgaac cgaaccgatc
     2221 acgccgttca gatcatgcaa gcggtcaaca gtcccaacgt aaagatttgc tttgacatct
     2281 atcaccagca gatcaccgag ggaaatgtga tccgaaacat ccgcgaaaac attcagtgga
     2341 tcggtcactt tcacgttgca gacaatccgg gacgcaaaga accaggtaca ggtgagctca
     2401 actaccgcaa tatcttcaaa gcgattgcgg agacaggcta ttctcgtttc gtcgccctgg
     2461 agtgcggtca ttccaccggc aactacgaag acacgcttcg cagaacgctg gaatgcctga
     2521 gttggacgta gccggtttcg aacgcttgtc ctgatgggtc gtttctgttg tggaagcccc
     2581 ttcctttcca aggaaaggct agaggaggtg gctttgattt gccgaccctt gggatgactg
     2641 ccgtatgtct atcgcgtgct gcaagcgctc aacagacaga ctaatatatt cagactcgag
     2701 ttcagttcct acgaattgcc ggcaggtatt cacggcagct acaccggtag ttgaactgcc
     2761 agaaaaagga tcgaagacca agtctcctct gctggttgat gccaggataa tgcgttccag
     2821 aagagagacg ggtttctgag tggggtgttt tccaaatccc ttttcatctt tattcggcgc
     2881 ggtcagagtc cacaccgact tcatttgctt tccattgttg gcttcacgca tcttgtcgta
     2941 gttgaagcag tgtttggcat cttggttctt agacgcccat ataatggttt ccgttgaatg
     3001 cgtgaagtac cgacacgaca ggtttggagg aggattcggt ttttcccatg taatgttgtt
     3061 cagtatcttc attcccagtt gttgcatggc gtaaccgaca gaatgaatga catggtgggt
     3121 gcctgccacc catattgttc cgttttgctt cagcagtttc tggcatctgg agagccacgt
     3181 tgtgttgaat tcgtgattga gttcaggacc acttgatctg tcccattgac ccttgtcaac
     3241 ctgaaccatt ttcccggcgt ggcaggtaat tccaccgttt gaaagaaaat agggaggatc
     3301 cgcaaaaatc atgtcgaaac agccgtctgg attctgaccg atgagcatct ccatgaactc
     3361 aatgcagttc gcctggtaga gccagacccc aacttgatcg ttgccgtaga cgcattggct
     3421 ggggggatac ggcaaatccc ctttggccat caatggaaag atggccttcc cttctatctc
     3481 atcctgtgtc acggcaatca ctttcttcat tcctgatgta tggcaagggt catgcggttt
     3541 gttcataata cctttgctgg agacagtgag gaacatcctc acacgtcgga attccacata
     3601 tcgagcgaaa accaatatag aagatcgcgg gtggtttatc aacgcgctcc caattatcgc
     3661 ttgtgactgt tgtgtgatat ggttaggata gtcttgtatt gttcacagtg cagtgtttca
     3721 taaaggagga cgtctcattg cgaatcagca gaatcctgct ctccttgttt gtgttctcac
     3781 aggtcgcgtt tgcgggaaac tttgttcgga tcagcccatc cggcaagtat tttcaactgg
     3841 aagatggaac cagttttgtt cccatcggat ccaatgaagc catgaactgg cctgagatga
     3901 tgtgtatcta ccccaatcat ggggagtaca atccacctgg tgcagaacac tacttccaga
     3961 tgcttagaaa acacggcgtg aactgcatcc gcatcatggc agaagcagcc aacacgggta
     4021 ttcgcaacgg tggcgcgttc cttgaggaac ctgtcgggga gtatgttcag gatacagttg
     4081 cctttctcga cggacttttt gaactggcga cgcaacacca tatttatgtc attctgacac
     4141 cctttgacac tttctggatg gacaagaaat gggatcagca cccttacaat gccgtcaacg
     4201 ggggaccggc gcattcgcga gaggagtttc tgacttcaga ggcggtgttc aaatgccaac
     4261 aggagcgaat gcgctctctg atcgatcgat tcggcaactc ggatttcctt tttgcctggg
     4321 agattctcaa tgaggtggat atgcgatggg gagcgacacc tgagcaacaa agggattgga
     4381 ctgcacgcat ggccaagttc gttcgggagt acgaaaagac aaaatggggt accaatcatc
     4441 tgatcacggt cagcctgact gaaaccgaag tcgaagcaga ctcccgcatt ccctccttgc
     4501 ttcttgacaa cccatttctg gactttgtga caacgcacat ctactatccg caagggagag
     4561 caccggtgga tccgatccag ccggcactgg agttcggtcg aggaattaga aggcagttgg
     4621 aacagattga gcagagtgtg cagcgtccct tcttcgcttc cgaatttggt cccgttgatc
     4681 tgtggcctct tccggacgaa ctcgatgatg aactatttca caatatgtcc tgggcacatc
     4741 tggcaacggg tgctgcgggg gctggtcttc gatggccgta tcgctggcat cacatcctct
     4801 ccgaggggat gagagagagc caactcggtt tgagccttgt tgcatcgaag attccctgga
     4861 gcacatttgc cgcacgaaat gttgatgaaa gactgagtgt ctccactccc gatgggcaga
     4921 aggtacatcc agtcggctgt ggcgatgaac agcagttcct gatatggctt gtccgcgagt
     4981 ccaactccaa gaaggttggg gacggtgata cgcagctgg
//
LOCUS       DAOQUC010000084         5014 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_18527_length_5014_cov_3.094374, whole genome shotgun sequence.
ACCESSION   DAOQUC010000084 DAOQUC010000000
VERSION     DAOQUC010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5014
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18527_length_5014_cov_3.094374"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1201
                     /locus_tag="PKH07_02350"
     CDS             <1..1201
                     /locus_tag="PKH07_02350"
                     /inference="COORDINATES: protein motif:HMM:NF014598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HNT33818.1"
                     /translation="FPQVVIPCVTLMILAVGMGYFVYRKGGNLTGATIVAIVLMALSV
                     WYGKDHPVTALPVLGEMTSQTWIYLLLAYALLASVLPVWLLLQPRDYLNCFQLYGGMI
                     LMTVAVLLTAPPIVAPMVNKAPMDVPPWFPFLFITVACGAISGFHSIVSSGTTAKQLD
                     KPADARSVAYGGMVTEGFLAVLAIIACTAGFASREVWLQTYSSYAGTDKLKLPTFVHG
                     AASIISQIGIPEALGATLISLTVVGFALTTLDTGTRLLRYNVEEIAKALGFRKTMNRY
                     LSSLIAVLAIGNIALVRIEGQSVGLILWQLFGATNQLLAGLGLLVATLYLLSRRRPFW
                     YTLIPMVFMVIVTGVALGINVRDFWRAGERLLTVVGSLILALTIWFVGEACLAYRKAR
                     RLSGAGS"
     gene            1444..2070
                     /locus_tag="PKH07_02355"
     CDS             1444..2070
                     /locus_tag="PKH07_02355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33819.1"
                     /translation="MYKVLVDGEHWDHQSSVVADLKGLLVHLTESISAKHRAIVRMAV
                     DGLELEGLDDMRLEGLSLDSVQTIEVNTENPRTLSIQILYEVAKYLARIADEMPVVAE
                     MIQSRDEKGAFERLQEVLGTWIEINRGLSNAAVALGINFDEMVIKGRSMSSVHKDVRT
                     SLDAAAQSLEQASYLELSDNLEYELAPKLRHLQECVYQMITIAEKRLN"
     gene            complement(2112..2543)
                     /locus_tag="PKH07_02360"
     CDS             complement(2112..2543)
                     /locus_tag="PKH07_02360"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNT33820.1"
                     /translation="MTQDADSAKMASSGRERRQHTRVPVQLPVIIRLKGGSSASKNVS
                     PIQAITRDVSEGGMYVELGERFLTPARNLAIDNFLLFRSDLEIELKLPIQPMPIHPVG
                     KAVWIEKKIPGRPFQHGIAICFKSIDDDHLKLLLEFLARVS"
     gene            complement(2685..4790)
                     /locus_tag="PKH07_02365"
     CDS             complement(2685..4790)
                     /locus_tag="PKH07_02365"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNT33821.1"
                     /translation="MAERLWLRLEYVLLCSLLLVLPSFMDTGYQTPTITPKVLLTVVV
                     SCILALLWALRRVIWRGTVGHFIAPLPFLLMGGYLAFHLIQSEYFHYALRASLYSFPA
                     LFLGLYTSVHVRGQRERTGVLLFLLLASLPVAVYAILQSFGVDYVQWDVNNDARQRIG
                     STLGNPSYVGDYLAVCMVLLVGLVLSIQKRWLRVVACLYFPIAGLAILRTLSRGPWLL
                     VTVGIISYLLMAGRFRELPRLLNRRVVLIALAVLVLVVIVLGFLVSRMEDTKVLLERF
                     REGIYLQESSVQLRLTVWEVTTRLWLKHFWLGSGLHQFRVLYLDELYELLVTRRSQAY
                     SSFLFKTNVVQADNAHNDYLQILAEWGVFGMAAWTLILATAFSLAFRRMRAEGHCTQQ
                     GSSHWQNFAAIASMITLLADAAYAFPFQLPVTNLMGFVLLGCMVGYGQEPARGAETRV
                     PRLVSALILITVIFLSGKALQQVWHIDRGERQILQALSAESAGKSDVALRHYQEAYHY
                     LPENGELSVLIGASYSRLGQMSTARDFYEKAHLTYNNPTLYLNRAMVALETRRYEECE
                     RLLERVESLVPNHPQVHYLRGLYLYLRGSFEDAEKEFRAELKNSKTDLNTLLYLGQTL
                     VHLEKSQEAENTFRRAVHVSPRNMVARENLGDLYSEQLNLPQQALTEYTIALEIARKS
                     GNGRAEYRIQRKLDQLQSQ"
     gene            complement(4808..>5014)
                     /locus_tag="PKH07_02370"
     CDS             complement(4808..>5014)
                     /locus_tag="PKH07_02370"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNT33822.1"
                     /translation="PLLESLCGRKIPVQHADWRPGDQEVFICDISSAKKDFGWEPRIS
                     LQEGVKQLYRWVEDNQDLIKKILG"
BASE COUNT         1200 a         1234 c         1356 g         1224 t
ORIGIN      
        1 gtttcctcaa gttgtgatcc cctgtgttac cttgatgatc ctcgcagtgg gcatgggtta
       61 ttttgtctac cgaaagggag gaaatcttac aggggctacg atcgtggcca ttgttcttat
      121 ggctctttct gtctggtatg gaaaagatca tccggtaacc gcattgcctg tattgggaga
      181 aatgaccagc cagacttgga tataccttct acttgcttac gcactcctag cttctgtgct
      241 tcccgtgtgg ctcttgctcc agccacgcga ctatctgaac tgttttcaac tgtacggtgg
      301 tatgattctt atgacagtgg ccgtgcttct gacggctcct ccgattgtcg cccccatggt
      361 caacaaagcg cccatggacg ttccaccttg gtttcctttt cttttcatca ctgttgcgtg
      421 tggagcgatc tctgggttcc acagcatcgt ttcctcagga actacggcca agcagttgga
      481 caaacctgcg gacgcgcgtt ctgttgccta tggcgggatg gtaacagagg gctttcttgc
      541 cgttttggcg atcatcgcct gcactgcggg tttcgcgtct cgggaagttt ggcttcagac
      601 ctattcgagt tatgcgggta cggacaaact gaaactgcca acctttgttc acggagcggc
      661 atcgatcatc agccagatcg ggatcccgga agccctcgga gcgactctga tctcgctgac
      721 tgtggttgga tttgcactga caacgctgga cactggaacg aggttgttgc gctacaatgt
      781 cgaggagatt gccaaggcgc taggcttcag aaagacgatg aaccgatacc tctcctcgtt
      841 gatagctgtc ttggcgatcg gaaacattgc tttggttcgg attgaggggc agtccgtggg
      901 gcttattctt tggcagctct ttggagcgac gaaccagttg ttggcgggtt tgggtcttct
      961 tgtggccact ctctatcttc tgtcacggcg tcgaccgttc tggtacacat tgattcccat
     1021 ggtttttatg gttattgtga ctggagtggc tctggggatt aacgtgagag atttctggag
     1081 agcgggagag aggcttctga cggtcgtagg gagtcttatc ctggctttga ccatttggtt
     1141 tgtgggtgaa gcctgtcttg cctatcgaaa ggccagacgg ctatcgggag cagggagcta
     1201 gagagaattt ctgacgaaga ggggaatctt ctgtttctga cacgcagggc atctcgagtt
     1261 gaaagtcccg aaggagagtg gttctgttgg ggtattggca ccacaagata gtgcacacga
     1321 gtctatcgga agaatgtatt ttgacggcct ctcatggatg cgatatattg aacaaactaa
     1381 aagcgcactc tgaagcgcga caaaaaccta tgttgactct gttgagtgaa aggacacagc
     1441 accatgtaca aggttcttgt tgacggtgag cattgggatc atcagagctc agttgttgca
     1501 gacctgaagg ggcttctggt tcacctgacc gagtccatct cggcgaaaca tcgtgctatt
     1561 gtgcgtatgg cggttgatgg cctcgaactg gaaggtctcg atgacatgag gcttgagggg
     1621 ctctcactcg acagtgtaca gacgatcgag gtgaacacag agaatcctcg caccctttcg
     1681 atccagattc tctatgaagt ggcaaagtat cttgcccgca tcgctgacga gatgccggtc
     1741 gtggctgaga tgatccagtc gagagacgag aagggagcct ttgagcgact ccaggaagtt
     1801 ttggggacgt ggatcgagat taaccggggt ctttccaatg ccgctgttgc actgggcatc
     1861 aattttgatg agatggtgat caagggacgt tcgatgtcat cggtgcataa ggatgttcgt
     1921 acttcattgg atgcggcagc gcagtctttg gagcaggcca gttatctgga gctttcggat
     1981 aacctggaat acgaactggc gccaaaactc agacaccttc aggaatgcgt ctatcagatg
     2041 ataacaatcg cagagaagag actgaactag cccagatgat tcatgtgtcc tgcttggcgc
     2101 tgagcgtgcc atcaagacac tctcgccagg aactccagta gcagtttgag atgatcgtca
     2161 tcaatgcttt tgaagcagat ggcgatgcca tgctgaaacg gtctgcctgg aatcttcttc
     2221 tcgatccaga ctgctttgcc aacgggatga ataggcatag gctgtatagg aagtttcaac
     2281 tcgatctcca aatcgcttcg gaaaagaaga aagttgtcga tcgccagatt tcttgcaggt
     2341 gtgagaaaac gctctcccaa ctcaacgtac attcctccct cactcacgtc gcgtgtaatc
     2401 gcctgaattg ggctgacgtt cttggaggcg gaggaacccc ctttgagtcg gattattacg
     2461 ggcagctgca ctggaacccg tgtgtgctgt cgtcgttctc ttcctgagga agccatcttg
     2521 gcgctgtctg catcctgcgt caaaaacact caccccttag ttctcgatct tgtaaggtcg
     2581 actgaatgac tctgcattaa gataccagaa aacatttgca tggcaagacc tgaatgaatg
     2641 tcttctcttc ctgccatttc cagaggtcca agggaagttc caagtcattg gctctgcagt
     2701 tgatccagtt tccgctgaat ccggtattcc gctcgcccgt tgccagattt tcgggcgatc
     2761 tccagcgcaa ttgtatactc agtgagtgcc tgttggggta ggttcagctg ttcactgtag
     2821 aggtcaccca gattctcacg tgcaaccata ttcctgggac tcacgtggac tgctcgtcgg
     2881 aacgtgttct ccgcttcctg acttttctcc aagtgaacga gcgtttggcc gagatagagt
     2941 agagtgttga gatctgtctt gctgttcttg agctcggctc ggaactcctt ttcagcatct
     3001 tcaaaagaac ctctaagata gagatacaag cctctaaggt agtgaacctg gggatgattt
     3061 ggcaccagag attcgacccg ctccagaaga cgctcgcact cttcatagcg tctggtttcc
     3121 aacgcgacca tggcacggtt cagatacaga gtgggattgt tgtaggtcag atgagccttc
     3181 tcgtagaaat ctcgagcggt actcatctgt ccgagtcggc tgtatgacgc gccgatcaga
     3241 acggagagtt cgccgttttc gggaagatag tgatatgcct cctgatagtg acgcagggcg
     3301 acatcgctct tgccagcgga ttcagcgctc agtgcttgaa gtatctgtct ttccccccgg
     3361 tcaatatgcc agacctgctg gagtgccttt cccgatagaa agatcaccgt aatgaggatc
     3421 aaggcggaga cgagtcgggg cacacgtgtt tccgctcctc ttgcaggttc ctgtccatag
     3481 cccaccatac atccgagaag gacgaagccc atgagattgg tgacgggcaa ctgaaatgga
     3541 aaggcatagg cagcatcggc aagaagcgtg atcatagaag caatcgcggc gaagttctgc
     3601 caatgactgg aaccctgttg ggtgcagtgg ccttcagctc tcatccgccg gaatgcaaga
     3661 gagaaggctg ttgccaggat gagcgtccat gccgccatgc cgaatactcc ccattcagcc
     3721 agaatctgaa gatagtcatt gtgggcattg tccgcctgaa ccacgttggt cttgaagaga
     3781 aaggaagaat aggcttggga acgtcttgtc acgagaagtt catacagttc gtccaagtat
     3841 aagactctga attggtgcag tccagaaccg agccagaaat gtttcagcca gagcctcgtc
     3901 gtgacctccc aaacggtcaa acgaagttga acactgcttt cctgaaggta gattccctcg
     3961 cgaaacctct ccaagagaac cttggtgtct tccatgcggc tgacgagaaa tcccagcaca
     4021 atcaccacga gcaccagaac ggcaagagcg atcaggacaa cacgcctgtt gagcagcctg
     4081 ggtaactctc tgaaacgtcc tgccatcagt agataagaaa tgatcccgac cgtaacgaga
     4141 agccaaggcc ctcgagagag agtacggaga atggcaagac ctgcaatggg gaaatagagg
     4201 caagcgacta ctctgagcca ccttttctga atagaaagaa ccagtccgac cagcaagacc
     4261 atacaaacag ccaggtaatc tcccacgtaa ctgggattgc ccaaggttga tccaatgcgt
     4321 tgtctcgcgt cgttgttcac gtcccattga acgtagtcaa ctccgaacga ctgcagaatg
     4381 gcgtagactg cgaccggcag agatgcaaga agcagaaaga gaagaacacc agtgcgttct
     4441 cgctggcctc gaacgtggac tgaggtgtag agaccaagaa acagggcagg gaaactgtag
     4501 agtgaggcgc gtaacgcata gtggaagtac tctgactgaa tgagatggaa agcgagatac
     4561 ccacccatca ggagaaaagg gagcggtgcg atgaaatgcc ctactgtgcc tcgccagata
     4621 actctgcgaa gagcccacaa gagcgccagg atgcacgaga cgactactgt cagaagaact
     4681 tttggagtga tcgtgggtgt ctggtagccg gtatccatga aactcggcaa aacgagcagc
     4741 aaactgcaca aaagcacgta ctcaagtcta agccacaaac gttctgccat gacatggggt
     4801 ttaccggtca gcccagtatc ttctttatca gatcctgatt gtcttcgacc caccgataca
     4861 actgtttgac gccttcctga agagagattc gaggctccca accgaaatcc ttcttggcac
     4921 tgctgatatc gcagatgaag acttcctgat cacccggcct ccaatctgca tgctgaactg
     4981 gaatcttcct tccgcaaaga ctctccagaa gagg
//
LOCUS       DAOQUC010000085         5008 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_18552_length_5008_cov_3.430446, whole genome shotgun sequence.
ACCESSION   DAOQUC010000085 DAOQUC010000000
VERSION     DAOQUC010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5008
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18552_length_5008_cov_3.430446"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..561
                     /locus_tag="PKH07_02375"
     CDS             <1..561
                     /locus_tag="PKH07_02375"
                     /inference="COORDINATES: protein motif:HMM:NF024376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HNT33823.1"
                     /translation="RFDLDPGGMRLRIHLSGRGPYASVLSRRELGISNINSLKGRTVA
                     AVHPLALTGGLSQLGYLASSGFNPVRDLDVHYCGSPEEAFKMLVAGWVEAAFVPEAAA
                     ESVFSESGLSGISLDRFPEIQRLGPVLGSVILFKRSLVEDDPVLDAHLREVLARQRGS
                     GTFVPAKREYYVTVREYLQAVGQVRE"
     gene            565..1806
                     /locus_tag="PKH07_02380"
     CDS             565..1806
                     /locus_tag="PKH07_02380"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNT33824.1"
                     /translation="MFRRSGQERRGILGFRFQVLAFVLIGFVALVLFRSVYLSNFENV
                     PQAVLVSADPTWLGASDELPAGFAVWENFERNILETSPVERLVVTQVDRNGRERVVHP
                     FHLMLTNPNYFTTQAEKATRLPISQGERVCGYLYVHLDRRHLTFFDTLFWTLFALILI
                     GAVAASMRFSFQEKSIESITSQLEQKKRQLADLEKLALVGQLTANVLHDLKKPLLNIR
                     DEVSSLPESESKASIRRQVELFFEMVRDLNIEGFLSRESLRDEFIDLQDVIEQSLRLV
                     RYEMGNVNASVRVSTDTPFILGVKHRLVQVFSNLILNSLEAMKGKGDLTIDCERYQDP
                     KGLFARARISDTGKGIPQDVIGHVFEPFYTYGKSQQSTGLGLYITREIIEEMGGTISV
                     ESIVDQGTFFTILLPATLDQE"
     gene            complement(1817..2269)
                     /locus_tag="PKH07_02385"
     CDS             complement(1817..2269)
                     /locus_tag="PKH07_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006965367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D domain-containing protein"
                     /protein_id="HNT33825.1"
                     /translation="MTLTAYCGCAKCCEWERGRWLYLKLNFWNRYVSKGARKGRPYRG
                     LTASGTKPREPAFGIISLDTGLHPWRLPARLILPPWHLFSHPGTLAADTDYYPFGTEM
                     LVPGYGWGVVEDRGGAIKGPERVDLYFRSHRRALKWGSKRLGVQVVNR"
     gene            2538..>5008
                     /locus_tag="PKH07_02390"
     CDS             2538..>5008
                     /locus_tag="PKH07_02390"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33826.1"
                     /translation="MATSKYVSVNEWLLLGSFPMVERQKGRWLENGYPCEAVFAPSTG
                     ETFDGRSWTVWKAEDVDLLDPSIAFQQRQWCFAYALTYVRSPKKQTVRLLMGSDDGIA
                     VWLNTEKVYQNDCQRVFEKFQDTVSVTLNEGWNRLLCKISQADAAWRFHVAVAGATER
                     SPRPGVLVSARRPAGKLLPSLKGRRSGIVLSGGVPSAKVMVDRDGMIFRCGLPLFNDR
                     DQSVRRVKATLADAAGRVLSQGAVGLLEPFVSQTLTLDVDGESFLDKLASGSGVILKT
                     ASSLGEGSLALNVSETLSTKLFLELMTGFEIPVSSGAQVFIPQVFRGVPGLVETYSNG
                     EHLPREIPWPKMPTQKITPQQSRTGRIQIDLVIPAGVPKAMGRISFGDEGLVEIQHKV
                     RYLCCVLQADISDCTSISEKGLQALGRKDFESAATALRDLYEHLAAKETDRCDQRVTL
                     IGHGHIDMNWLWTSWETLQCCHDTFRQVLAFMEEFPDFKYSQSQASTYQYIERIDPAM
                     FRKIQERVREGRWEILGGTVDEGDTNLSSGEALARSLLLGQRYFREKFGRIARVSWCP
                     DNFGHAAQLPQILGLAGLKYFYGHRCQPKIGCSNWVGPDGSRLINYSTPTYNGEVDNR
                     LREAPAEYDPEHKSMIWVYGVGDHGGGPSRQDITRAIALQDMPGFPEIRFGTAEEFFK
                     SKESQAKDYPTYQGELQYIFEGCYTSIARIKEGNRRCENLLYATDMLVALMGLDGYPF
                     PQEQLRDAWHKLTFNQFHDILCGSATHEANRESIALYDAAIAIAEEVCFCGLRQLASR
                     VSKKRNNGQPLVVFNSLGAPRTDVV"
BASE COUNT         1171 a         1171 c         1419 g         1247 t
ORIGIN      
        1 cggtttgacc ttgatccagg cggtatgcgt cttcggattc atctatctgg caggggtccc
       61 tatgccagcg ttctctctcg tcgagaactt ggtatctcaa acataaattc tctgaaagga
      121 cgcaccgttg cagcagtcca tcctttggcg ttgactgggg gactctcgca gctgggatat
      181 ctggcaagtt ccgggttcaa tccagtgcgt gatcttgacg tgcattactg tggcagccct
      241 gaagaggctt tcaagatgct tgttgcaggt tgggtggagg cggcctttgt ccccgaagca
      301 gcagcagaat ctgttttctc tgaatcaggt ttgtctggaa tctcactgga caggttcccc
      361 gagatacaaa ggctggggcc tgttttggga agtgtcattc tgttcaaacg gagccttgtt
      421 gaggatgatc cggttcttga tgctcatctg cgggaggtac tggctcgaca aaggggaagt
      481 ggaaccttcg tgcctgccaa gcgtgagtac tatgtcaccg tgcgcgaata tcttcaggca
      541 gtgggacaag tcagggagta agacgtgttt cgaaggagtg gccaagagcg cagaggcatt
      601 ctgggattcc gtttccaggt tcttgccttt gttctgattg gatttgtcgc gcttgtgctt
      661 tttcgaagtg tctacctctc caatttcgag aatgttccgc aagcagttct ggtctcagcg
      721 gatccgacat ggttgggagc ttcagatgaa ctgcccgcag gatttgccgt ttgggagaat
      781 tttgaacgca acattctgga gacaagtcct gtagagcgtt tggtggtgac ccaggtagac
      841 cgcaatggca gagaacgggt ggttcatcct tttcacctta tgctgaccaa tccgaactac
      901 ttcacaacgc aagctgaaaa ggcaacccga ctgcccatct ctcagggaga acgagtctgt
      961 ggttatctct acgttcatct tgatcggaga catctgacct tctttgacac actattctgg
     1021 acactttttg cactcatact catcggtgct gtggctgcgt caatgcgctt cagttttcag
     1081 gagaagagta ttgagagcat cacttcgcag ctggagcaaa agaagaggca gcttgctgac
     1141 ctggagaaac tggcacttgt aggacaactc accgcaaacg ttctgcacga tctgaaaaag
     1201 ccactcctga acatcagaga tgaggtttct tcgctgccgg agagcgaatc aaaggcgtcc
     1261 atcaggcgac aggtggaatt gtttttcgag atggtacgcg atctcaacat cgaaggattt
     1321 ctttctcgag agtcgcttcg ggacgagttc attgatcttc aggatgtcat tgaacagtca
     1381 ttgcggctgg tccgctatga gatggggaat gtgaacgcat cagttcgcgt gtcaaccgac
     1441 accccattta ttcttggagt caaacatcgg ttggttcaag tgttttcaaa ccttatcctg
     1501 aattctttgg aggcaatgaa ggggaaggga gacttgacga ttgattgcga gaggtaccag
     1561 gatccgaaag gactctttgc gcgagcccga atttccgaca cgggcaaagg gattcctcag
     1621 gacgtgattg gtcacgtctt cgagccattc tatacgtacg gaaagtccca gcagtctacg
     1681 gggttgggtc tttacattac ccgcgagatc attgaagaga tgggggggac aatcagtgta
     1741 gaaagcattg tggatcaggg aacattcttc actatcctcc tgccagccac tctcgaccag
     1801 gagtaagttt cggattctag cgattgacga cttgtactcc gaggcgtttg ctgccccact
     1861 tgagcgcacg gcggtgagaa cggaagtaga ggtcaacacg ttcgggacct tttatcgcgc
     1921 ctccccggtc ttccactact ccccatccat agcccggcac gagcatttca gttccaaatg
     1981 gatagtagtc tgtatcagcg gccagagtcc caggatgcga gaatagatgc cagggaggca
     2041 gaatgagtcg tgcggggagt ctccagggat gtaaacccgt gtccaatgaa atgatgccga
     2101 atgcaggctc tcttggcttc gttcccgatg cggtcaagcc tcggtacggt cgtcccttcc
     2161 gagctccctt ggagacataa cgattccaga agttcagttt gagataaagc cagcgcccac
     2221 gctcccattc acagcacttt gcgcagccac aataggcggt gagtgtcatc tgcttctgac
     2281 gcacggtggc gcagccagag aagcacaata cgagcaccgt caacagaaga acttgtctga
     2341 ctctgtttga gaaataggac atcatattcc aagtatccca caaaccgaag aggacacaaa
     2401 gcgttttgtt ggaacaaggt attgtgtctg aagcaacaaa atgcaccatt acttgaagcg
     2461 aaacgtataa tccgctataa cacccgctgt ttgcatgtca gcaaggtctg agagaaaggt
     2521 aaaggttgtt ctcttctatg gctacttcga aatatgtttc cgtgaatgaa tggttgcttt
     2581 taggttcgtt tccgatggtg gagcgacaga aggggagatg gcttgagaac ggctatccat
     2641 gtgaggccgt ttttgcgcct tccacaggtg aaacatttga tggtcgaagc tggactgtct
     2701 ggaaggctga ggatgttgat ctattggatc cttcaatcgc ctttcagcag agacagtggt
     2761 gctttgccta tgccctgact tatgttcgtt ctccgaagaa gcagaccgta agactgttga
     2821 tgggtagtga cgatgggatc gctgtctggc tcaacacaga gaaggtttat cagaacgact
     2881 gccagcgcgt ttttgagaag tttcaggata cggtaagcgt gacgctgaac gaaggctgga
     2941 accggcttct gtgcaagata tctcaggcag atgcagcttg gcgatttcat gttgccgtcg
     3001 ctggagcgac agaacgcagt ccaaggcctg gggttctcgt gtcagctcga cgtcccgcag
     3061 ggaaactgct gccgagtttg aagggacgaa gaagtgggat tgttctttct ggtggagttc
     3121 cctctgccaa agtgatggta gaccgcgatg gtatgatctt ccgatgtggg cttccgttgt
     3181 tcaatgatcg cgatcagtca gtgcgacgtg tgaaggcgac tcttgccgat gcagcaggac
     3241 gggttctgag tcagggagca gtcggtttgc tggagccatt tgtcagtcag acgcttacgc
     3301 ttgatgtgga cggtgaaagt tttcttgaca aactggccag tggctcagga gttatcctga
     3361 agacagcgtc atctcttgga gaaggcagtc ttgccttgaa tgtgagcgag acgctgagca
     3421 caaaactgtt tctcgaactg atgactggtt ttgagatacc tgtttcttca ggagcacaag
     3481 tcttcattcc tcaagtgttc cgaggggtgc cgggtctggt tgaaacatat tccaacggcg
     3541 aacatctccc gagggagatt ccctggccaa agatgcctac tcagaagatc acgccacaac
     3601 aatccaggac gggtaggatt cagattgatc tggtcatccc tgctggagta ccaaaagcca
     3661 tggggaggat ttcatttggg gatgagggtc ttgtggagat tcagcacaaa gtgcggtatc
     3721 tgtgctgtgt gctccaggcc gacatttccg actgcacatc tatttctgag aagggtcttc
     3781 aggcgctggg tcgtaaggac tttgaatcgg cggcgacggc tctcagagat ctttatgagc
     3841 accttgcggc aaaggagacc gatcgctgtg atcagcgggt gactctgatc ggacatggcc
     3901 atattgatat gaactggctc tggacatcgt gggagacgct gcaatgctgt catgacacat
     3961 ttcgacaagt tttggccttc atggaggaat tcccggactt caagtactcc caaagccagg
     4021 cttcaaccta tcagtacatc gagcgaattg acccggcaat gtttcgaaag attcaggaaa
     4081 gggttcggga aggacgttgg gagattctgg gaggaacagt tgatgaaggg gatacgaatc
     4141 tgagttccgg agaagcgttg gcacggtcgc ttcttctggg acagcgttat ttcagggaga
     4201 agtttggacg aattgcccga gtgtcttggt gtccagacaa cttcggccat gccgcacaac
     4261 tcccacagat cctggggctt gccggtctga agtacttcta tggacatcgt tgtcaaccga
     4321 agattggctg ttccaactgg gttggtcccg acggaagcag gctcatcaat tactctactc
     4381 cgacctacaa cggggaagtg gacaaccgtc tgagggaagc accggccgaa tatgatcccg
     4441 aacacaagag catgatctgg gtctacggag ttggcgacca tggcggaggc ccaagtcgtc
     4501 aggacatcac gcgggccatc gcattgcagg atatgcctgg atttccagag atacgttttg
     4561 gaactgccga ggaattcttc aagtcaaagg aatctcaggc aaaggactac ccaacgtacc
     4621 agggtgagct ccaatacatt ttcgaggggt gctatacatc cattgcgcgg atcaaagagg
     4681 ggaaccggcg ctgtgagaac ctgctctatg cgaccgatat gcttgtggct ctgatgggac
     4741 ttgacggata tccgtttcca caagagcagt tgagggacgc ctggcacaaa ctgacattca
     4801 accagtttca cgacattctt tgcggttcgg cgacgcacga agcgaatcgg gaatctattg
     4861 ccctctatga cgccgcgatt gccatcgcgg aggaagtgtg tttttgtgga ctgaggcagc
     4921 ttgccagtcg ggtttccaag aaacgcaaca acggccagcc tttggtggtt ttcaactcct
     4981 taggtgcgcc gagaaccgat gtggttga
//
LOCUS       DAOQUC010000086         5004 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_18583_length_5004_cov_3.125884, whole genome shotgun sequence.
ACCESSION   DAOQUC010000086 DAOQUC010000000
VERSION     DAOQUC010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5004
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18583_length_5004_cov_3.125884"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1761
                     /locus_tag="PKH07_02395"
     CDS             <1..1761
                     /locus_tag="PKH07_02395"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNT33827.1"
                     /translation="PGIVPRSSNISLVQATTSADNLNEHRIHSTRWGVIAHLAIIFVA
                     VFICYANTLNHNFVYDDKDEMLENPIIHSLRNVPRFFTTSTYGYYRPLRTLVWAIDYA
                     FWKEKPSGYHMTNLILYVFACWSLYWFLVLLFQNHAQALITSLLFLTHPIHTESIAWI
                     SGRGDPLAATFLFLSLCCTIRWAHNSPARGRSKGFGLWAVAMILFVFSVLSKESGITL
                     PIILIALTACSHREQPLVRRRLLWSATASGVILLLLILLRFHVLRSLTPSNSPINDSR
                     WNNVLFAAMAFWRYVGLMAVPVNLSADYPAAESLILNGSSVVSAILGHIAVTTLVAVL
                     IVKRRTGACFAIVWIYATYLPVSNLVPLNQSFSEKYMFLPVLGMCILLGTLWTLPLPK
                     TQTRTLRIFLGVLLIFYTVATIQRNKVWKDEVTLWTRTLSDQPESGLAAYNLACEYDR
                     LGQSEKAIQYYLRAISLRPEDARQYQNLGNLYLNLNRMTDAIECYKKTISMGLVTPHV
                     YAALGAAIASLGHLKQGQAIVEKSLDMNPNYVPGLYNLSLILRDRGMTDEADKVLERA
                     HQLNPSVGTQRRGWIQTRNN"
     gene            complement(1743..2912)
                     /locus_tag="PKH07_02400"
     CDS             complement(1743..2912)
                     /locus_tag="PKH07_02400"
                     /inference="COORDINATES: protein motif:HMM:NF013067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33828.1"
                     /translation="MKTLVLIPSSKTLRYAYFVRGTSTNTTSGNLRRFRGAEASQEAI
                     DEINKALILKGYSLECGENCLYDLVAIRVCYGGKEFSQGELVTSDVVRKLERLTEDSP
                     IHIPAVLSLIGEVRRAFSTVPVVLVFETSFFARLPVRESCYALDDKLLGGSRLRRTGF
                     HGIFHTAAVQTALLERDRADRSRAKRILSICLDRRSEVCAVAGRTPLTVTSGVTPLEG
                     LPGERTCGEIDPSIVLSLSQELGLGPEEINDVLSHQSGILGMTGRRISISTLFKSSSP
                     EVVAARDVFQYRLLLAAGSGISAMGGIDHIVFSGRYSRTGRLLEHWLLGKIAESCRSE
                     APRPQVSYCTDSLDRLVANQAIAASRQAELTEKRVGRIADMRVLHENTACQLFRV"
     gene            complement(2909..4141)
                     /locus_tag="PKH07_02405"
     CDS             complement(2909..4141)
                     /locus_tag="PKH07_02405"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006083 - acetate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HNT33829.1"
                     /translation="MNVLVFNCGSSSLKYRLIAMPEETELAGGEAQRIGPPTAEPSRI
                     VHHIGAERETITVPMASHAEAFNQVMQILSRESAMTVHAVGHRVVHGGEWFECPTKIG
                     SAEYDLLQKTSSLAPLHNPPAMNLIDACRTLYPYLDQVAVFDTAFHSSIPIHARSYAL
                     PQPLVARLGLRKYGFHGISHQFVLEQAAKFLCRDTKEMNAVSCHLGSGGASLCAISKG
                     RSIDNTMGYSPLQGLVMSTRCGDIDPAIALQLLTLNHGDHSVVEKAFNKSSGVLGMSG
                     ISADIRDVLSFAGDSERSSTRINLTRQVYLWRIRKYLGAYLLALGKADAVIFTDTIGE
                     LVPEVRWNVCAGMDFFGLEISHTLNEDVMEFPADISKPGSAVRVLVIQTNEELAIARR
                     TYRTMAPDSDSSRQGAAA"
     gene            complement(4175..>5004)
                     /locus_tag="PKH07_02410"
     CDS             complement(4175..>5004)
                     /locus_tag="PKH07_02410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33830.1"
                     /translation="LRGNLVVAATVAEENGLSVGVRYLVEKTFPELELTPNYAVLGEP
                     TGLNLYYGHDGWMELDVCIEGANPFHVDDTATTILRDFHSREKSLSEMHDREILSVHG
                     STFENRQGVHRATIQVSRRLSEHEESESVAGEIQRVLAAQESGDVAVAVAVRQERQSL
                     YSGKATLVRRVTHAWSTDPFSPIMTRSRHALAAAGCYCTPGKWRLNRLGMGTAGSVLV
                     NEFGIPVVGYGPGTEAMAHAVGECVSVPNVHECAYGTASIVHSLIGVPVCGWTSDEI"
BASE COUNT         1162 a         1421 c         1230 g         1191 t
ORIGIN      
        1 ccaggcatcg ttccaaggag tagcaacatt agcctcgtgc aagcgaccac atctgctgac
       61 aacttgaacg aacatcgcat ccattcgact cgctggggtg tcatcgctca tctggcgatc
      121 atttttgtgg cggtctttat ctgctatgcg aacactctca accataattt tgtctacgat
      181 gacaaagatg agatgctcga gaatccaatc atacattcac tcagaaatgt cccccgattc
      241 ttcactacgt ccacctatgg ctactacagg cctttgcgga cactggtttg ggcaatagac
      301 tatgcgttct ggaaagagaa accatctgga taccacatga ccaacttgat cctgtatgtt
      361 ttcgcctgct ggagtttata ctggtttctt gtgctcctgt ttcagaatca cgcccaggcg
      421 cttatcacca gccttctctt tttgactcac cccatacaca cagaatcaat tgcatggatc
      481 agcggacgag gcgatcctct ggctgccact ttcttgttcc tgtctctgtg ctgcactatc
      541 cgatgggcgc acaacagtcc ggctcgcggt cggtcaaagg gttttgggtt gtgggctgtg
      601 gcgatgattc tctttgtttt cagcgttctc tccaaggagt ctggaatcac tttgccgatc
      661 atcttgatcg cgcttacggc ttgcagtcat cgtgaacagc ctctggttcg acgaagactt
      721 ctctggtctg caactgcttc gggtgtgatc ctgctgcttc taatactcct gcgttttcac
      781 gtcttgcgga gcctgacgcc ctcgaacagt ccgatcaatg actcccgttg gaataatgtt
      841 ctctttgcgg cgatggcatt ctggagatac gtcgggctga tggcagttcc cgtcaatctc
      901 tccgcagact atccggccgc ggagtcactc attctgaatg gcagcagtgt tgtctcagcg
      961 atactaggtc atattgcggt gaccactctt gtggctgtgc tcatcgtcaa gagaagaacc
     1021 ggtgcttgct tcgccattgt ctggatctac gcaacctatc ttccggtcag caacctcgtg
     1081 ccactgaatc agagtttcag tgaaaagtat atgttccttc ccgtcctggg aatgtgcatc
     1141 ctcctcggca cactctggac tctgcctctg ccgaagacac agactcgcac gttgcggatc
     1201 ttccttggtg ttcttctgat cttctacacc gtggcaacga tccagcggaa caaggtctgg
     1261 aaggatgagg tgacgctctg gacacgcaca ctgtccgatc agccggagtc tggtctggct
     1321 gcgtataatc tggcttgtga gtacgaccgc ctcgggcagt ccgagaaagc gattcagtac
     1381 tacctgagag ccatcagtct tcgtccagag gatgctcgtc aatatcagaa cctgggcaat
     1441 ctctatttga atctcaatag gatgaccgac gccatagaat gctacaagaa gacaatctcc
     1501 atgggcttgg tgacccctca cgtctatgcg gctcttggtg cagccattgc atcactcgga
     1561 catcttaagc aaggacaagc gatcgtcgag aagtcgcttg acatgaatcc caactatgtt
     1621 cccggtttgt acaatctgtc cctgatcctt cgcgataggg gtatgactga tgaggctgac
     1681 aaggtcctcg aaagagcaca ccagttgaat ccgtctgtcg gcacacagcg ccgcggctgg
     1741 attcagacac gaaacaactg acaagcagta ttctcatgaa ggacacgcat gtccgcaatt
     1801 cgtccaactc tcttctctgt aagttctgcc tgtcttgagg ctgcaatagc ctgatttgcg
     1861 acaagacgat caagagagtc agtacagtag ctcacctgag gtctaggagc ttccgagcga
     1921 catgattctg cgatcttccc caagagccaa tgctcaagaa gacggcctgt tctactatac
     1981 cgacccgaga agacaatatg gtcaattccg cccatcgccg aaatgccact tccagcagca
     2041 agcagcaggc gatactggaa aacatctcgt gcggcaacca cttcggggga gcttgacttg
     2101 aaaagggtgg agatgctgat gcgtctgcca gtcatgccca gaatgccact ctggtgtgaa
     2161 aggacgtcgt tgatctcctc aggacccaga cccagctcct gagagagtga aaggacaatg
     2221 ctcgggtcga tctcaccaca cgttctctct cccggaagac cctcaagtgg ggtgactcca
     2281 ctcgttacag tcaaaggcgt tcggccagca acggcacaaa cttctgaacg acggtccaga
     2341 cagatggaaa gtatcctctt tgcccggctg cgatctgctc tatcgcgctc aagtagggcg
     2401 gtctggactg cagcggtatg aaagatgccg tgaaaaccag tccgcctgag gcggctacca
     2461 ccaagcagct tgtcatccaa cgcatagcaa ctctcacgta ccggaagtcg tgcgaagaag
     2521 gaagtctcaa aaacaagcac aaccggcact gtcgagaagg ctcttctcac ttctccgatc
     2581 agagagagca ccgccggaat gtggattggc gaatcctcgg tcagcctttc cagtttcctg
     2641 acaacgtctg aggtaaccaa ctcaccctga gagaactcct tgccaccgta gcagacacga
     2701 atcgcgacaa ggtcatatag acaattctcc ccacactcca ggctgtatcc cttcaggatg
     2761 agagccttgt tgatttcgtc aatcgcttcc tgcgaggctt ctgctccacg aaatcttctt
     2821 agatttccgg aagtcgtatt ggtgctcgta cctcttacaa agtacgcgta tctcagggtc
     2881 ttacttgacg gaatgaggac gagagtcttc atgctgctgc tccttgtctg gaagaatccg
     2941 aatccggagc catcgtccgg taggttctgc gggcgattgc caactcctca ttggtttgga
     3001 taaccaagac tctcactgca gatcctggct tggaaatgtc tgccgggaac tccatcacat
     3061 cttcattgag tgtgtgcgat atttctaagc cgaagaagtc catcccagca cagacattcc
     3121 accgaacttc gggaacgagt tcgccaatcg tgtcggtgaa tatgacagcg tctgctttgc
     3181 cgagtgcgag aagataagct ccgaggtact tgcgaattcg ccagaggtat acctggcggg
     3241 tgagattgat acgagtgctt gagcgttcgc tgtcacctgc aaaggaaaga acatcacgga
     3301 tgtccgccga gatgcctgac atccccagga caccgctgct cttgttaaag gctttctcga
     3361 caacactgtg atccccgtgg ttgagagtca gcaattgaag agcaattgca gggtcaatgt
     3421 caccgcatcg ggtactcatg acgagccctt gcagcggaga ataccccatc gtgttgtcaa
     3481 tggatctacc tttggatatg gcacacaggc tggcaccccc gctgcccaga tgacagctca
     3541 cagcgttcat ctcttttgtg tccctgcaaa ggaacttcgc tgcctgctca aggacaaatt
     3601 ggtggctgat tccatggaaa ccgtatttac gcaacccaag gcgtgcaacc agtggttgtg
     3661 ggagagcata gctacgcgca tggatcggta tgctcgaatg aaaggccgta tcgaatacag
     3721 ccacctgatc caagtaggga tacagtgtcc tgcacgcatc aatgagattc atcgctggtg
     3781 gattgtgaag gggggcaaga ctggacgtct tctgaagcag gtcatactca gccgagccta
     3841 tcttcgttgg acactcaaac cattctccac catgaaccac acgatgcccc acagcatgca
     3901 ccgtcatcgc ggactcccga ctcagaatct gcatcacctg gttgaatgct tcggcatggc
     3961 ttgccatggg cactgtgatc gtctcgcgct ctgcacctat atgatggaca attcgcgaag
     4021 gctccgcagt cggcgggcca attcgctgtg cctccccacc agccaactcg gtctcttcag
     4081 gcatggctat cagacgatac ttcagggatg agcttccaca gttgaacaca agaacattca
     4141 tcgagtaaac ctccgtcctc ctatcgcgct tccgtcagat ttcatcggat gtccaaccac
     4201 acaccggaac tccaatcagg ctgtgcacaa tggacgctgt cccgtatgcg cactcatgga
     4261 catttggcac agagacacac tctccaaccg cgtgtgccat cgcctcagtt cctggcccat
     4321 acccaacgac aggaatcccg aattcgttca cgagtacact gccggcagtt cccatcccaa
     4381 gcctgttcag tcgccacttg ccaggggtgc agtagcagcc cgcagcagca agggcatgac
     4441 gcgaacgtgt catgattgga ctaaacggat ctgttgacca ggcatgagtg acacgccgaa
     4501 ctagcgtagc cttgccactg tacaagctct gtcgctcctg cctaacggca acagccacag
     4561 cgacgtctcc tgactcctgt gcggcaagga ctctctggat ttctccagca acagattcag
     4621 actcctcgtg ctcagacaat cttcgggaaa cctggattgt ggctcgatgc actccctgtc
     4681 tattctcaaa cgtcgagccg tgaacagaga gaatctcacg atcatgcatc tcggagagac
     4741 tcttctcacg cgagtgaaag tccctcagta tcgttgttgc tgtatcgtca acatgaaagg
     4801 gattcgctcc ctcgatgcaa acgtccagtt ccatccaacc gtcgtggcca tagtagagat
     4861 tcagccccgt cggttcacca agaactgcat agttcggagt caactccagt tccggaaacg
     4921 tcttctcaac aagatatctg acgcccacgc tcagtccgtt ctcctcggca acagtggcgg
     4981 cgaccacaag gtttccgcgc aggg
//
LOCUS       DAOQUC010000087         4959 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_18820_length_4959_cov_3.833401, whole genome shotgun sequence.
ACCESSION   DAOQUC010000087 DAOQUC010000000
VERSION     DAOQUC010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4959
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18820_length_4959_cov_3.833401"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2580)
                     /locus_tag="PKH07_02415"
     CDS             complement(<1..2580)
                     /locus_tag="PKH07_02415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33831.1"
                     /translation="MKTLIPVFVLVLSVSCVVPKAPDSKVLSQAMPSVHDRLLQDYDT
                     VLSRAPHFFERGWTEGYQGDPSLGFFAEGGHGELSMRTLGNFITTYAFLARHPGYDEE
                     LGGVSRAELERKALAAIRYMIRTNTTGDLVCTTGFKWGDDPLGRDGWESSWYASKMTT
                     GAVMIWDALNEADKEGIRKVLASESDRSARVPPPTGTRSDTKSEENAWDCESLAWALV
                     LFPDDVRAATWEHTLYDYAMNTLSVPQDADDHSVVGDKTVSQWFRGPNVHPDFTIENH
                     GFFHICYQICPLHSLAWSVYAYVQAGKEPPPCLFHHFEDVWRELRKYYLWDGRFAYVG
                     GKDWPRYVYGMNFIFPALALVQNVYADDCASAIERKRLDLLKWEQEYHGDGSFFSGRF
                     TNLDMFGRHVEYESDVYADMILAYRLHQIDWPESLRMPKQFDPPLGTTFSQHSNHILV
                     RGERRFASWSWNPRVKPAQGIFAIRGMESALEWDGNLFGRTYCDGFVPEAEIVHTHTV
                     PIPGGFATAGLVREGLTRDNIRLIDDVSVGFIPVLTDHPLLNKPHRIGTVDRLVSHDS
                     IERISENWSVIAKSQEGRPCLVSRKVGKGEIVVCMANAGQLYLEESDPCLLFENLVHY
                     FAGSRVGYLLGEETGEQALAKAGISFDTITDLGKVKLKDYDWILAGRNSFDRVRYKAH
                     VQEFREYLFNGGGLITLVLQDNGFDSRWLTFGETSAEPEHAVDHSLAFIALPDDMTCL
                     RYDFLRANISVRARLEGLRWLIGNDIFNGNKRTINGEEMVGVGGSDRVVLLNYPKVNV
                     DDRLFLTVLQGEGYPAVRDAAQRNVIWKSLLVEAICYPWWETPGEFAQGQVIQRTVVS
                     LDGR"
     gene            2827..3591
                     /gene="dapB"
                     /locus_tag="PKH07_02420"
     CDS             2827..3591
                     /gene="dapB"
                     /locus_tag="PKH07_02420"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020458821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HNT33832.1"
                     /translation="MGIGVLVNGAKGKMGREIVLAVQSQPDMELIAQTDLGDDLSGIL
                     RDLKIDVAVDFTHPDCAFRNARLFVESGKSAVIGTTGFKETELRRLHEMALDKNVGLL
                     IAPNFCTGAVLMMRFAEEAARFMNSVEVVEMHHTAKVDAPSGTAKLTADKIAQVWKSR
                     GIDPVKLNKINEFRGGLVSGIPVHSVRLDGLMARQEVMFGGDGQTLEIRHNSQSRSCF
                     MPGILQSVRAVRGWKGLVYGLDRLLFAGKDGIQTGL"
     gene            3601..4323
                     /gene="rsmG"
                     /locus_tag="PKH07_02425"
     CDS             3601..4323
                     /gene="rsmG"
                     /locus_tag="PKH07_02425"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF014575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HNT33833.1"
                     /translation="MACLSDYDKRLRVYAERLRVAAVSFNLVSRLELDRIELHILDSI
                     MAFNNRLKMNELEEQAALGIDIGSGAGMPGIVLSVLFPEISWVLLESRKKRLDFLNET
                     LSQLGISNARAVRGRVEELGHESPWRASANIATARAVAPFPVLAEIGVPLLKVGGRLL
                     CMKGHQVKQEIDGCVEFLDSLGASVEDIHTYESPLGGTRAVVSIRKDAETPDHFPRSA
                     QRLRQESKRFHLKQAKQEDCFT"
     gene            4320..>4959
                     /locus_tag="PKH07_02430"
     CDS             4320..>4959
                     /locus_tag="PKH07_02430"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HNT33834.1"
                     /translation="MKHRVMKYGVPLLGCLKILITMEMLAVNNAQAQLQTVTRYCNPL
                     DVIIADPFVLRDGDLYYLYGTSAPGEGYHVWTSPDLVNWWWRGFAFQKTASSWGQSHF
                     WAPEVLKWKDKFYLFYSAHGVTQSLRLGLAASESPLGPFVDIKTPLFDSDKAWIDGHP
                     FVDSDGTPYLYFVKDVSENPQSEIYVAEMQPDLMGLKEPFTFCLKPSGGWEGN"
BASE COUNT         1177 a         1278 c         1332 g         1172 t
ORIGIN      
        1 tcttccgtcg agggagacca cggttcgctg aatcacttgt ccctgtgcaa actctccagg
       61 ggtttcccac cagggatagc agatggcctc tacgagcagg ctcttccaga tgacgttgcg
      121 ctgagcagca tctcgaactg ctggatatcc ttctccctga agtacggtga gaaacaatcg
      181 gtcatccaca ttgaccttgg ggtagttcag aagcacaacg cgatcagagc cacccactcc
      241 caccatttcc tctccattga tggtgcgttt gttgccattg aaaatgtcgt ttccaatgag
      301 ccaacgaagc ccttcgagcc gagcccttac agatatgttc gctcgaagga aatcatagcg
      361 caggcaggtc atgtcatcgg gaagtgcgat aaaagcaaga gagtgatcca cagcgtgctc
      421 cggttctgcg ctggtttcac caaaagtcag ccatcggctg tcaaagccat tgtcctgaag
      481 aacgagagtg ataagaccac cgccgttaaa cagatattca cgaaactcct gcacatgcgc
      541 tttatatcga acgcgatcaa agctgtttct gcctgcaaga atccagtcat agtctttgag
      601 tttcactttc cccaagtctg tgatggtgtc aaatgagatt ccggctttcg cgagagcctg
      661 ttctccagtt tcctctccaa gcagatagcc cacacgggaa ccagcgaaat agtggacgag
      721 gttctcaaac agcagacagg gatccgactc ctcaagatac agctggcctg cattggccat
      781 gcaaacgact atctctccct tgccaacctt tcgagacaca agacacggcc tgccttcctg
      841 gcttttcgcg atcacagacc agttttcgga aatgcgctcg atggagtcat gactcaccaa
      901 tctgtcaacg gttccgattc gatgtggctt gttcagaaga ggatgatctg tgagcactgg
      961 gatgaaacca acggaaacat catctatcag gcggatgtta tcccgagtca gaccttcgcg
     1021 aaccagaccg gctgtggcaa agccaccagg gattgggacg gtatgcgtat ggacgatttc
     1081 cgcttccgga acaaagccgt cacaatatgt gcgtccaaag aggttgccat cccattccaa
     1141 cgcactttcc atgccacgaa ttgcaaagat tccctgtgcc ggtttgactc gaggattcca
     1201 gctccaggat gcaaatcgcc tctcaccacg tacgaggata tggttgctgt gttgactgaa
     1261 cgtcgtgcca agaggcgggt caaattgctt gggcatccgc agggattcag gccagtctat
     1321 ctggtgcagt cggtacgcca gaatcatatc cgcgtagaca tcggattcat attccacatg
     1381 ccgtccaaac atgtcgagat ttgtaaagcg accggagaag aacgaaccgt ctccatgata
     1441 ctcctgctcc catttcagaa gatccaagcg tttgcgttct atggccgacg cacagtcatc
     1501 agcgtagacg ttctgcacca gagccagtgc cgggaagatg aaattcatcc cgtacacata
     1561 ccttggccag tcctttccgc cgacgtaggc aaaacgaccg tcccagagat agtactttct
     1621 caactcgcgc cagacgtcct caaaatgatg gaacaaacat ggaggtggct ccttgcccgc
     1681 ttgcacgtag gcatagacac tccacgccag agagtgcagt ggacaaatct gatagcagat
     1741 gtggaaaaat ccgtgattct ctatcgtgaa atccggatga acattgggac ctcgaaacca
     1801 ctggctgact gtcttgtccc caacaacact gtggtcatcg gcatcctggg gaaccgagag
     1861 cgtgttcatg gcatagtcgt acagggtgtg ctcccaggtt gcagcacgga catcatccgg
     1921 aaacagtacc aatgcccatg ccaacgattc gcagtcccag gcattttcct cggacttggt
     1981 gtcactgcgt gtgcctgtgg gaggtggcac ccgagcagag cgatccgact cagacgcgag
     2041 cacttttcga atgccttcct tgtccgcttc attgagcgcg tcccagatca tcacggcacc
     2101 cgtcgtcatc ttcgaggcgt accaggaaga ctcccagcca tcgcgtccca gaggatcgtc
     2161 tccccatttg aaacccgtcg tgcagacgag atctcctgtc gtgttggttc gaatcatgta
     2221 tcggattgcg gccagggcct tgcgttccag ttccgcacga gatacgcccc cgagttcctc
     2281 atcgtaacca gggtgtctgg cgagaaacgc gtatgtcgtg atgaaattcc ccaaggttcg
     2341 catggagagt tccccgtgtc cgccctcagc gaaaaatcca agagacggat ctccttgata
     2401 cccttccgtc caaccacgct caaaaaaatg cggagccctt gacaacaccg tgtcgtagtc
     2461 ttgcaggaga cggtcatgga cagagggcat cgcctgactc agcacctttg aatcgggcgc
     2521 cttcgggaca acacaggaaa cactaagaac cagaacaaac acagggatca gggtcttcat
     2581 tttcaacact ccattctgcc gtatttcccc atgacaccct gacacagaca gtccttacga
     2641 cccgcagaac agaaacacta cgagcaatga ggtatcgtgt caaatcggat tggagagaag
     2701 agatgattct gttagattca tctactgaaa gacagatttt tggttgtctt gcggagcaag
     2761 cgttcaaggg agtgggtatt gcccacgtgc ctgcaggcgc tggccagcgc agaaggagca
     2821 tcaacggtgg gtatcggtgt actggtaaat ggagccaaag gaaagatggg tcgcgagatc
     2881 gtgctggcgg ttcagtcgca accagacatg gagttaattg cacagaccga ccttggcgac
     2941 gatctgagtg ggattcttcg cgatttgaaa attgatgttg cagtggactt cacgcatcct
     3001 gactgtgcct ttcgaaatgc gagactgttt gttgaatcgg ggaagtcggc tgtcatcggc
     3061 acaacaggat tcaaggaaac cgaactccgt cggttgcatg agatggcatt ggacaagaac
     3121 gtgggactct tgattgcgcc gaatttttgc actggagccg tactgatgat gcgtttcgcg
     3181 gaagaagcgg cgcgattcat gaacagtgtt gaagtggttg aaatgcatca cactgcgaaa
     3241 gtggatgcgc cgtccggcac ggcgaaactg accgccgaca agatcgccca agtctggaag
     3301 agcaggggta ttgacccggt taagttaaat aaaatcaatg agtttcgtgg tggattagtc
     3361 tccggaatcc cagtgcacag cgttcggctg gatggtttga tggctcgaca ggaagtcatg
     3421 ttcggtggtg acggtcagac gctggagatc cgtcataact cccagagtcg ctcctgcttc
     3481 atgcctggca ttctgcaatc ggtacgtgca gttcggggct ggaagggctt ggtctatggt
     3541 ttggacagac tgctctttgc cggtaaggat ggaattcaga cggggctttg agtggcactt
     3601 atggcatgtc tttcggacta tgacaagcgt ctgcgggtat acgccgagcg actgcgtgtg
     3661 gctgctgtaa gtttcaatct tgtctcccgc ttggaacttg accgaatcga actgcatatt
     3721 ctggattcga ttatggcttt caataacaga ttaaaaatga atgagttaga agaacaggcc
     3781 gccttgggaa ttgacatcgg ctccggtgcc gggatgccgg gcattgttct ttctgttctg
     3841 tttcccgaaa tctcttgggt tctgcttgaa tcccggaaaa agcgactgga ttttctcaat
     3901 gaaacattga gccaacttgg gataagcaac gctcgagccg ttcggggtcg agtggaagaa
     3961 cttggacatg agtcgccgtg gagggcttct gccaacatcg ccaccgctcg cgctgtcgct
     4021 cccttccctg ttttggcaga gattggcgtg ccgctgctca aagtaggagg gagacttctg
     4081 tgtatgaagg ggcaccaggt caagcaggag atagatggat gtgttgaatt ccttgattcg
     4141 cttggcgctt cagtggaaga tatccatacc tatgagagcc cgttgggagg tacccgagcg
     4201 gtcgtttcta tccggaagga cgccgaaacg cctgatcatt ttccccgatc agcccagagg
     4261 ctgcgccaag agtccaaacg ttttcacctc aaacaagcga agcaagagga ttgtttcacg
     4321 tgaaacacag agtcatgaag tacggtgtcc ctctcctggg ttgtttgaag attctcatca
     4381 ccatggagat gctggccgtg aataatgccc aagcgcaact gcaaaccgtc acgcgatact
     4441 gtaatcccct cgacgttatc attgcggatc cttttgtgct cagggatggg gatctctact
     4501 acctctatgg aacatccgcg ccgggcgagg gatatcatgt ttggacatca cctgacttgg
     4561 tgaattggtg gtggcgtggt tttgcctttc agaagacggc ctccagttgg gggcagtctc
     4621 atttctgggc accagaagtt ctcaagtgga aggataagtt ctaccttttc tacagtgccc
     4681 atggggtgac gcagagcctt cgtcttgggc tggcggcaag tgagagtccc ctgggtcctt
     4741 ttgtggatat caagacacct ctgtttgact ctgacaaggc ctggattgat gggcatccgt
     4801 ttgtggactc agacggcacc ccttatctgt acttcgtcaa ggatgtctcg gagaatcctc
     4861 agagcgagat ctatgttgcc gaaatgcagc ccgatttgat ggggctgaag gagccgttca
     4921 ctttctgtct caaaccgagc ggtggttggg aaggaaact
//
LOCUS       DAOQUC010000088         4956 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_18837_length_4956_cov_3.659661, whole genome shotgun sequence.
ACCESSION   DAOQUC010000088 DAOQUC010000000
VERSION     DAOQUC010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4956)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4956)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4956
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18837_length_4956_cov_3.659661"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            218..1258
                     /locus_tag="PKH07_02435"
     CDS             218..1258
                     /locus_tag="PKH07_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNT33835.1"
                     /translation="MSKGFKVGVIGLGMGRHHLRCFHEHPSSKVVAIADQEPQRLKEI
                     GDQYGIKKRYSDGAEMIEKEKLDVVSIATPNKFHCPLTVHAFKKGCHVFCEKPMAMTV
                     EEAEKMNRAAQKAKRSLMINFSYRFSEMSFALKQQVDSGVIGNVYFGRTVWHRRRGIP
                     KLGGWFTTKELSGGGPLIDLGVHRIDLALWLMGYPEPQSVFGSAYNVLAKEIARAQNK
                     QYSVEDMACGLVKFKNGATLIVEASWALNVHQPEYMETALYGDKGGLVHRNTDGGYTM
                     GAELYLEEGGSLFTKKLDRACVKVPTPYGEFIDSLVEGRQPIATGEHGLKVQKILNGL
                     YQSAEQGAPVTF"
     gene            1331..3424
                     /locus_tag="PKH07_02440"
     CDS             1331..3424
                     /locus_tag="PKH07_02440"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007360156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HNT33836.1"
                     /translation="MSQVSFDSRALKIDGKRTLIFSGAIHYPRSTPAMWPDLMKRSRA
                     AGLNTVETYVFWNLHEPKRRVYDFSDRLDLLQFCRVAQQHGLYVILRIGPYICAETNY
                     GGFPFWLRDVPDMQMRTFNTPFMLEMGRWVRFLCNHLNRMFAPNGGPIIAFQLENEYS
                     LVALHYGDSGQRYLRWTVDLAQELNLGIPLIMCCGSAPGAIETLNGFVMHPKVQEHVK
                     NHPGQPVLWTENWPGWYDTWGYAHRSRSAEHVAYSVARFFAEGGAGVNYYMWHGGTNF
                     GREAMYLQTTSYDYTAPLDEYGLPTTKSNHLTQLHHVLWEHADLLLEKSRVEPQLVGD
                     NQWIYVYENGTDSLAFLCNDDENKSATISHLGQGYELPALSVILISKGKVLMNTARVA
                     PVSVVQRSVEPISDILAPFECWAEPLPTDWPEGVRAPIRSAAPEEQLQFTQDETDYCW
                     YETRLNIAPSEAGKGELLLEGVADVVYVFVDGQICATTPVPLPEQRGKVDEEGFHQKF
                     ELHLNAGTHDLSLLCCAVGLIKGDWMIGYENMVNERKGLWGKARWKGRTLHEPWRIQP
                     GLVGEHCRLFGSAGALVPWQPDGTPQRGKPLCWLRTRFSRPEGLSPLTLDLVGMGKGL
                     IWLNGHCLGRYWLTPGLGDMNPSKRSVIEEQRVNEPTQRYYHVPSEWIQDENVLVLFE
                     EVGGDPSLIRLCRWL"
     gene            3497..4873
                     /locus_tag="PKH07_02445"
     CDS             3497..4873
                     /locus_tag="PKH07_02445"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HNT33837.1"
                     /translation="MSRSYDFLVLQNRLCRIILLACLLSGIPAAFCQQQTEQSLPRSN
                     QRSVLFIVVDALRADALGVNGSKVPATPFLDKLATETWCFTNCRSSASLTKASMCSLW
                     TSNYPSVHAVISRTDKLDDRFQTLPEIMKDCGLRTTLVSGNPWLSDEFGMSQGFDEES
                     LINYAHDMGRDFDGNDLNETFLKWIDRQPNVPFFAHLHYMDCHDPYNATAEDMKQFVQ
                     VDGKDFSVNGPITSEMRSGDLAYMRQHYQAAVRSLDRIISRLFQELENRQLLSNTIIV
                     LTADHGEEFGEHGGLGHGTTLYDELLRVPLMIHVPGEPPARLEGITSLVDVLPTLREL
                     MGIPSSADDVGVDLTQYVKGQPLPERAIHARVQSLSDYSELYAAIWNDHKFIFNRSKG
                     TKEFYDLRADPKERSPLRPEQSPLYAEGEKLVQTQMAVADRNPNASAPVTILPDTLNA
                     LKSIGYAK"
BASE COUNT         1142 a         1236 c         1422 g         1156 t
ORIGIN      
        1 agccaaaatc aaggggagac agatcaagga atggacgctt gaacacaatg cggcgggggc
       61 gccgcctgaa aggccgattt ccagcgatgc gcctttgcgg gaactggaac tgattcccta
      121 cggatgcacg atgcttcggg tcacagagtt tccggtactg gagtgacacg aaaactggtg
      181 agtgatagaa aagactagat atctgggagc caaaacaatg tcgaaaggtt tcaaagttgg
      241 tgtgattggt ctggggatgg gaaggcatca tctgcggtgc ttccatgagc atccgagttc
      301 caaggtcgtg gcgatagcgg atcaggagcc gcaacgtctg aaggaaatcg gcgatcagta
      361 tggcatcaag aaacggtatt cagacggtgc cgagatgatc gagaaagaga aactcgatgt
      421 ggtcagcatt gccactccga acaagtttca ttgtcctctg acagttcatg ctttcaaaaa
      481 gggatgccat gttttctgtg aaaagcccat ggcgatgacg gttgaggaag ccgaaaagat
      541 gaatcgggct gcgcaaaagg ccaagagaag cctgatgatc aacttctcct accggttcag
      601 tgagatgagt tttgccctga aacagcaagt ggactccggt gtcatcggga acgtctattt
      661 tggtcggaca gtttggcatc ggcgtcgcgg tatccccaaa ctgggggggt ggtttaccac
      721 caaggagttg tccggaggtg gaccgctcat cgatctggga gtccaccgaa ttgacttggc
      781 gctttggctg atgggctatc ctgagccgca atcggtcttt ggctctgcgt acaatgtcct
      841 tgcaaaggaa atcgcgcggg cgcagaacaa acagtattcc gtggaggaca tggcttgtgg
      901 cttggtgaag ttcaagaatg gagcaactct gattgttgag gccagttggg cgttgaatgt
      961 acatcagcct gaatatatgg agacggcgct ctacggcgac aagggcggtc tggtacatcg
     1021 caatacggac ggtggctaca ccatgggtgc tgagttgtat ctcgaagagg gtgggagtct
     1081 tttcaccaag aaactggatc gcgcctgcgt gaaggttcca accccctacg gcgagttcat
     1141 tgacagtttg gtcgaaggac ggcaacccat cgctacggga gagcatggac tgaaggtgca
     1201 gaagatactg aacgggcttt atcagagcgc agagcaggga gcaccggtta ccttctgaga
     1261 aggcacgtgg gctgttctgt gctcctgtgc agtactcgct tgcgttcgat catgaaaggg
     1321 tttgatttac atgagccagg tttcctttga ttcaagagca ctgaagattg atggcaagag
     1381 gacgctgatc tttagcggtg ccattcacta tccacgcagc acgccagcga tgtggcctga
     1441 cctgatgaag cgaagccgcg ctgctggtct taacaccgtg gaaacgtatg tcttctggaa
     1501 tctgcacgaa ccgaagaggc gtgtctacga tttctctgac aggctcgatc ttctccagtt
     1561 ctgccgtgtt gcccagcagc atgggctcta cgtcatcttg aggattggcc cttacatctg
     1621 tgccgagacc aactatggcg gattcccctt ctggcttcgc gatgttcctg acatgcagat
     1681 gcgcacgttc aacactccct tcatgttgga gatgggtcgc tgggtacgat tcctgtgcaa
     1741 tcatctcaac cgtatgtttg ccccaaatgg cggcccgatt attgcctttc agttggagaa
     1801 cgaatacagc ctggtggcgc ttcattacgg tgacagtggc cagcgctatc tgcggtggac
     1861 ggtggacttg gcgcaggaac tgaatctggg cattcctttg atcatgtgct gtggtagtgc
     1921 gcccggagcc atcgagacgc tgaatggatt tgtcatgcat ccaaaagtgc aggaacacgt
     1981 caagaatcat ccgggtcagc cggtgctctg gactgagaac tggccggggt ggtatgacac
     2041 ctggggctat gctcatcgtt cccgctcggc ggagcatgtt gcctacagcg tggcgcgttt
     2101 ttttgcggaa ggtggtgccg gcgtcaacta ttacatgtgg catggcggaa ccaatttcgg
     2161 ccgcgaagcc atgtatctgc agaccaccag ctacgactat accgccccgc tggatgaata
     2221 cggtctgcct accacaaaga gcaatcatct gacacaactg catcatgtgc tttgggaaca
     2281 tgccgatctg ttgttggaga agagccgagt cgagccgcag cttgtcggtg acaaccagtg
     2341 gatctatgtg tatgagaatg gcactgattc gcttgcgttt ctctgcaacg atgatgagaa
     2401 caagtcggca acaatctctc accttggtca ggggtacgaa ctgccggctc tgtccgtcat
     2461 cctcatctcc aaaggcaagg ttctcatgaa cacggctcga gtcgcacccg taagtgttgt
     2521 tcaaagaagt gttgagccga tctcggatat ccttgcgccg tttgagtgct gggctgaacc
     2581 gttgcccacg gactggcctg agggtgttcg cgctccaatc agatctgcgg cacccgagga
     2641 gcaactgcag ttcactcagg atgaaacaga ctactgttgg tatgaaacaa ggctcaacat
     2701 tgctccctcc gaggctggca agggagaact cttgctggaa ggagtggcgg acgtggtgta
     2761 cgtctttgta gatggccaga tttgtgccac gacgcccgtc ccgctccccg agcagagagg
     2821 caaagtagac gaagaaggat tccatcagaa gtttgaactg catttgaatg cgggaacaca
     2881 tgatctgtcg cttttgtgct gtgcggtcgg tctgattaaa ggtgactgga tgattggcta
     2941 tgagaatatg gtcaatgagc ggaaggggct ttggggaaaa gcacgctgga aggggcgcac
     3001 tcttcacgaa ccctggcgga tccagccggg acttgtgggc gagcattgtc gcctttttgg
     3061 aagcgccgga gcactcgtgc cgtggcagcc cgatggcaca ccccaacgcg gcaagccgct
     3121 ttgctggttg cgaactcgtt tctcgcgccc ggaaggcctg tcaccactca cgcttgatct
     3181 cgttggtatg ggcaaaggtt tgatctggct caatgggcat tgcctcgggc gctattggtt
     3241 gacccctggc ttgggagata tgaatccatc gaaaaggtcg gttatcgaag agcagagagt
     3301 gaacgagccg actcagcgtt actaccacgt tccgtcggag tggattcagg acgagaatgt
     3361 cctggtgctt ttcgaggaag ttggtggcga tccgtcactg attcgcctct gcagatggct
     3421 ctagtcagcc atcgtgcctc tgatctgtgc tgaaggaggg aggacatccg tctctggtcg
     3481 gatgtctaag aaacacgtgt cccgttctta cgactttctt gttctgcaga atcgtttgtg
     3541 ccgaatcatt cttctggcgt gtttgctctc gggaattcct gccgctttct gtcaacaaca
     3601 gacagagcag agtcttcctc gcagtaatca gcgaagcgtc ttattcattg ttgttgacgc
     3661 cttgcgtgct gacgcccttg gcgtgaatgg atctaaagtt cctgcaacgc cctttctcga
     3721 caaacttgcc acagaaacct ggtgcttcac caactgccga tccagtgcat ctttgaccaa
     3781 agcatccatg tgcagccttt ggacttccaa ctacccaagt gtccacgctg tgatctctcg
     3841 gacggacaaa ctggacgaca gattccagac gcttccagag ataatgaaag actgcggtct
     3901 ccgaactact ctggtgtcag ggaatccctg gctctccgac gagtttggga tgtctcaagg
     3961 cttcgatgag gaatctctga tcaactacgc tcacgatatg ggcagagact tcgacggcaa
     4021 cgatctcaat gagacgtttc tcaagtggat cgaccggcag ccgaatgtgc ctttctttgc
     4081 acaccttcac tacatggact gccacgatcc atacaacgcc acagcggaag acatgaagca
     4141 gttcgtacaa gtggacggga aggatttttc tgtcaatggc cccatcactt cagaaatgcg
     4201 gtctggtgat cttgcctaca tgaggcagca ttaccaggca gcagtgcgct cacttgacag
     4261 gattatcagt cgtctttttc aggaattgga aaaccgtcag ttgctgtcga acaccatcat
     4321 cgttctgacc gccgatcatg gcgaggagtt cggtgagcac ggtggtttgg gacacggcac
     4381 cactctgtac gacgagcttc ttcgtgtgcc gttgatgatc cacgttccgg gagagccacc
     4441 agcacgattg gaagggataa cttctctggt tgacgtgtta cccacactgc gtgagctgat
     4501 gggaatccca agttctgccg acgatgtcgg tgtggactta acgcaatacg tcaagggaca
     4561 gccattgccc gagcgggcga ttcacgcacg ggtacagtcg ttgagcgact actccgaact
     4621 ctacgcagcc atttggaatg accataagtt catcttcaac agatccaagg gcaccaagga
     4681 attctacgat ctgcgtgccg atcccaaaga gcggagtcca ctcagaccgg agcagtcgcc
     4741 cttgtatgct gagggagaga aacttgttca gacacagatg gcggttgccg accggaaccc
     4801 aaacgcatcg gcaccggtca cgattcttcc cgacacactc aatgcactca agagtatcgg
     4861 ctatgccaaa tgagtcgggg tgattgcatt cgcctcggaa cgcacaatag gctatcctat
     4921 gaatgaagga taaagaggaa gactcccgca aggaga
//
LOCUS       DAOQUC010000089         4940 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_18916_length_4940_cov_2.973183, whole genome shotgun sequence.
ACCESSION   DAOQUC010000089 DAOQUC010000000
VERSION     DAOQUC010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4940
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18916_length_4940_cov_2.973183"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1108)
                     /locus_tag="PKH07_02450"
     CDS             complement(<1..1108)
                     /locus_tag="PKH07_02450"
                     /inference="COORDINATES: protein motif:HMM:NF024739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNT33838.1"
                     /translation="MHGKEEKSGISTAVLFCVFVAVLGLPFLLRPQEAIRPSNALPLV
                     IISPHNEAIRYEFGRAFSDWHERRYGVPVDVDWRNVGGTSEITRYLGAQFASAFRAHW
                     QALGHSWTSQAATSFSNPKVDPHDESLSDAIREARREFLTSDVGVGIDLFFGGGEYDH
                     SRQAAMGHTVPCGYRDTPEGHEVLEKEIPETISGERWCDSEDRWYGATVSSFGICYNT
                     DILQLMPLTTAPSCWRDLGQPELFGTVALADPTKSGSMMKAFEMVIQQEMADAVAGLS
                     PDSPEYASQLGKGWAEGLNVIRRAAANSRYVTDSASKVTWDVALGDASVGMCIDFYGQ
                     FQAEAVSLPGGASRMHYVSPKGGTTVSCDPIAMLR"
     gene            complement(1126..2199)
                     /locus_tag="PKH07_02455"
     CDS             complement(1126..2199)
                     /locus_tag="PKH07_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020150773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNT33839.1"
                     /translation="MRVLVNGISKRFGSVSALRGVRLEVEHGEFFFLLGPSGCGKTTL
                     LRILAGFEEPDEGTLHFDEEDVTRLAPERRGAAMVFQSYALWPHLTVEENIAFGLEVQ
                     GTAQNEKRRRVGEMLDLVRMGGYEKRRPGQLSGGQQQRVALARALVVQPRLVLLDEPL
                     SNLDARLRLQMRTEIRQIIKDSGHTAVYVTHDQKEALSMADRCAILDQGVLVQVGRPR
                     EVYERPTNRFVADFLGEINFLSGRIASSQGNGMYTVQMDQINRLWSGIAQEELGIGAQ
                     VLCGIRPECWKVDDHASPGSNTLNGSVVSSVYLGEVTQHCVACSAADGGDIRVLEIHS
                     PVRRPGPVTLVAEPESTIVLKSE"
     gene            complement(2214..3422)
                     /locus_tag="PKH07_02460"
     CDS             complement(2214..3422)
                     /locus_tag="PKH07_02460"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNT33840.1"
                     /translation="MKSLNILMVFPQLPYPPESGGRIVTFEMVRFLLQRHQVRIASLQ
                     HRSEDIESAIHLEKMGAKVTMEKAPSRKSVPRLLRSVFSDCPYKAHRFYSKQLDLRIQ
                     NHLNLERFDVIHAQNFYMAQYIKGTEPCRKVYYKENYEGLLLKRYAATLPNPVTRMFW
                     QREAEKTSRFETNLGRRFDATLCISPADAERLRAASPDIDWQSIAPCINLDEHCYRRR
                     EQSPPMLLFLGMLNYFPNVDAAEYFVRKIFPAVRNRVSGVEVCFCGHSPDSRMQRLSK
                     YPGVKVIGSVDDPSDFMERCSVFIIPLRIGGGVRLKLLQALAKGCAVVSTSIGSEGLA
                     FEHRKHLLIADEPDAFADGVVQLLKDAALRESLGLQGHRQVEQHYSPSIVLPSLERVY
                     LGENNLSGRV"
     gene            complement(3485..4723)
                     /locus_tag="PKH07_02465"
     CDS             complement(3485..4723)
                     /locus_tag="PKH07_02465"
                     /inference="COORDINATES: protein motif:HMM:NF024758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HNT33841.1"
                     /translation="MRKLFMGSSTRVLLLAIVFLGLVLHPFAVFAQDLPYVLGEDTVG
                     GVEIKGPVRYAVPEEEILKNAVPAVVKLMVRLPQGDNTIGTGFFAGNGLLIATAHTLS
                     GQPIATIKTQEGKSFDARVVASDPKVNLVVLRASEASIAYLPLGRAFQVSAGDELMAV
                     TWPNIGSPRDVPLTIPRFLQFPNEMNVRKTKVLIARQKLSRATYWDTPQPAMVVDLVM
                     RPEWSGSPLIDQHGDVVAVCMGAEYGKKDSNTSQQMPYTVSVEVFQGLPPVVLESASE
                     PPAIPKQMSDEDRSRVMNLWRVWVMKAKVSKVPRIQMGGVEVPLTDKPEQIESFLVSQ
                     GGNEKPQVRQVAAGQAAQRATRASLVNWIFRPNPRIPFRVNVEYAVQDQSLQALPAPE
                     GGGYAIPIGFVRRAFRSYLP"
     gene            complement(4720..>4940)
                     /locus_tag="PKH07_02470"
     CDS             complement(4720..>4940)
                     /locus_tag="PKH07_02470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33842.1"
                     /translation="SGIYSESLAEVLQEGASSGVAEVSSENAPVIQDGSPETTLSKNE
                     GVPVIISIEIGRRLLDKSPTTRKQRTIE"
BASE COUNT         1212 a         1420 c         1199 g         1109 t
ORIGIN      
        1 ccctcagcat ggcaatcgga tcacagctga cagtcgttcc ccctttcgga gaaacataat
       61 gcattcggct cgcaccaccc ggcaatgaaa cggcctccgc ttgaaactgg ccgtagaaat
      121 caatgcacat ccccacagag gcatcaccaa gagctacgtc ccaggttact ttcgatgcgg
      181 aatctgtcac gtagcgggaa ttggccgccg ctcttcggat cacattcaga ccctcagccc
      241 aacctttgcc gagctggctt gcatactcag gtgaatccgg actgagacct gcaactgcgt
      301 ctgccatctc ttgctgaatg accatctcaa acgctttcat catagagccg ctcttggtcg
      361 gatctgcaag tgccacagtt ccaaacaact ccggttgtcc caagtccctc cagcagctcg
      421 gagccgttgt caaaggcatc aactgaagaa tgtccgtgtt gtagcagatc ccgaacgacg
      481 agactgtcgc accataccac cggtcttccg agtcacacca tcgctcgccc gagattgtct
      541 ccggaatctc cttctccaag acctcatgcc cttctggagt gtctcggtaa ccacagggca
      601 ccgtatgccc catcgctgcc tgtctggaat gatcgtattc tcctccaccg aagaagagat
      661 caatcccgac gccaacatct gaggttagga attcgcgacg tgcctctctg atcgcgtcag
      721 agagcgattc atcgtgggga tctacttttg gattactgaa tgaagttgca gcctgggaag
      781 tccaggagtg accgagagcc tgccaatggg cgcgaaatgc cgaagcaaac tgggctccca
      841 gatacctcgt gatttccgac gttccaccaa cgttgcgcca gtccacatca acagggactc
      901 cataccggcg ctcatgccag tcagaaaagg ccctgccaaa ctcataccgt atcgcttcat
      961 tgtgcgggct gatgatgacc aaagggagtg cgttcgaggg ccggattgcc tcctgaggcc
     1021 tcagcagaaa aggaagtccc aatacggcaa caaagacaca gaacaaaact gccgttgaga
     1081 tacctgactt ctcttctttg ccatgcatgg aaaacgcccc gtatcctact ccgatttcag
     1141 aactatcgtt gattctggtt ccgccacaag tgtcacgggt cccggccggc gaacaggaga
     1201 atgaatctcc agaactcgaa tgtcaccacc atccgccgca gagcaggcca cacaatgctg
     1261 cgtcacttct ccaagataaa ctgaggacac gacactccca ttcaaagtgt tgcttcctgg
     1321 acttgcatga tcgtcaactt tccaacattc cggcctgatg ccgcagagaa cctgtgctcc
     1381 gataccaagt tcttcctgcg caatcccact ccacaaacga ttgatctgat ccatctgaac
     1441 tgtgtacatg ccattgcctt gtgacgaggc tatcctgcca gacaggaaat tgatctcccc
     1501 aagaaagtct gccacaaaac ggttcgttgg tctctcatag acttcccgag gcctgccgac
     1561 ttgcacgaga acaccctgat cgaggatcgc gcatcggtca gccatggaaa gcgcttcttt
     1621 ctgatcatga gtgacgtata ccgctgtgtg accagagtcc ttgatgatct gacgaatctc
     1681 cgtgcgcatc tgcagtcgaa gtctcgcatc aaggttggac aacggctcat ccaagagcac
     1741 cagtcttggc tgaaccacca aagcacgagc cagggcaacc ctttgttgct ggccaccaga
     1801 caactggcct ggacgacgct tttcgtatcc ccccatacgc acgagatcaa gcatttcccc
     1861 aactcgcctt ctcttctcat tctgagcagt tccctgcact tccagcccaa aggcaatgtt
     1921 ctcctcaacc gtcagatgag gccagagcgc atagctctga aaaaccatcg cggcaccacg
     1981 cctctcaggc gcaaggcgag taacgtcctc ttcgtcaaag tggagtgtcc cctcatcagg
     2041 ttcttcaaat ccggcgagaa ttcgaagcag ggtcgtcttg ccgcagccgg aaggtccaag
     2101 caggaagaag aactcgccgt gctcgacttc aagacgaaca cctcgaagtg cagaaaccga
     2161 cccaaatctc ttggagatcc cattcaccag aactctcata cccttatccc gccttaaacc
     2221 cttccagaga gattgttctc tcccagatag actcgctcca gagatggcaa cacgatcgaa
     2281 gggctgtagt gctgctccac ctgacgatgg ccttgcagtc ctagcgattc gcgcaaagca
     2341 gcatctttga gcaactggac aacgccatcc gcaaaagcat ctggttcatc tgcgataagc
     2401 aagtgtttcc ggtgttcgaa agccaaaccc tcacttccaa tgcttgtcga gacaaccgcg
     2461 cagcccttgg ccagcgcctg aagcaatttg agtctcacgc ctccacctat cctgagcggg
     2521 atgatgaaga cgctgcatcg ttccataaag tcacttggat cgtcaaccga gccaatcact
     2581 ttgacgccag gatactttga caaacgctgc atcctgctgt ctggtgagtg gccgcagaaa
     2641 cacacttcaa caccagaaac acggtttctg actgcaggaa atatcttccg cacaaaatac
     2701 tcggcagcat ccacattcgg gaaatagttc aacattccca gaaaaagtag cataggtgga
     2761 gattgttcac gcctccggta gcagtgttcg tcaagattga tgcatggggc aatcgactgc
     2821 cagtctatat cgggactggc ggcacgaagc ctctctgcat ccgctggcga gatgcagagt
     2881 gtcgcatcga agcgacgacc cagattcgtc tcgaaccgtg atgtcttttc tgcttcacgc
     2941 tgccagaaca tgcgtgtcac aggattggga agggtcgcag catatctctt gaggagaaga
     3001 ccctcatagt tctccttgta gtaaaccttt cgacatggtt cagtaccttt gatgtactgt
     3061 gccatgtaga agttctgggc gtggatgacg tcaaagcgct ccaggttcag atgattctgg
     3121 atcctgagat ccaactgctt gctgtagaaa cggtgcgctt tgtaggggca gtcggagaag
     3181 acagagcgca acaaacgcgg aaccgatttc cgggaaggcg ctttctccat agttactttg
     3241 gcgcccattt tctcgagatg tatcgctgat tcgatgtcct cggaacgatg ctgcaaagag
     3301 gcaatccgga cttggtgtct ttgaagcagg aatcgaacca tctcgaaggt gacaatgcgt
     3361 ccaccactct caggcgggta ggggagttgc ggaaagacca tgaggatgtt gaggcttttc
     3421 attagaaggc ggacaacccc tatcagagcg gatgatcaat tgagtcgaaa gtcctgccaa
     3481 agcactacgg tagatagcta cgaaatgccc ttcttacaaa gcctatgggg atcgcgtagc
     3541 cgccgccctc tggcgcaggc agtgcctgaa ggctttgatc ttgaacggca tactcgacat
     3601 tgacgcgaaa aggaattcta ggattcggac ggaaaatcca gttgacgagt gaggcacgtg
     3661 tcgctctctg agcagcctgg ccagcggcaa cctggcggac ttggggcttc tcgttaccac
     3721 cttgcgagac caggaaagac tcgatctgct ctggcttgtc ggtcagcgga acctcgacac
     3781 cacccatctg aattcttgga accttgctga ctttggcttt catcacccaa acccgccaga
     3841 ggttcatcac acgtgagcga tcttcatcgg acatctgttt tggaattgcg ggtggctctg
     3901 aggcactctc caagacaact ggaggaagac cttgaaagac ttcaacgctg actgtgtagg
     3961 gcatctgctg ggaagtattc gagtccttct tgccgtactc ggcgcccatg cagacagcca
     4021 ctacgtctcc gtgctgatcg atgagcggac ttccactcca ctcaggcctc attaccagat
     4081 ccacaaccat ggcgggctgg ggcgtatccc aatacgtggc acgcgaaagt ttttgtctgg
     4141 ctataagaac tttcgtcttt cgaacattca tctcatttgg aaactgaaga aatctcggaa
     4201 ttgtcagggg aacatctcgc ggcgacccta tattcggcca cgtaacagcc atcagttcat
     4261 ctcccgcact gacctgaaaa gccctcccga gcggaagata ggcaatcgaa gcctccgagg
     4321 cacgaaggac aacgagattc accttgggat cagatgcaac gacacgtgca tcaaatgact
     4381 tcccttcctg agtcttgatt gtggcaatag gttggccact cagagtatgc gccgtggcaa
     4441 tgagcaatcc gttgccagca aaaaagcctg ttccgatagt attgtcgcct tgtggcagcc
     4501 tgaccatcag tttgacaact gcgggaactg cattcttcag gatttcctcc tcgggaacgg
     4561 catatctgac cggtcccttg atctcgaccc cacctacagt gtcttcgccc aatacataag
     4621 gtaagtcctg agcgaatact gcaaaaggat gaagcacaag tcccaggaac actattgcca
     4681 gaagcaacac tctggttgat gatcccataa agagttttct cattctattg tcctctgttt
     4741 ccttgtagtt ggagacttgt caaggagtct gcggccaatt tcgatggaga tgatgactgg
     4801 cacgccttca tttttcgaga gggtcgtctc cggtgaccca tcctgaatca ccggggcatt
     4861 ctcagaagaa acctcggcaa ctccggaaga cgccccctcc tgaagaacct ctgctagaga
     4921 ctcggaataa atacctgaga
//
LOCUS       DAOQUC010000090         4927 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_18981_length_4927_cov_2.897989, whole genome shotgun sequence.
ACCESSION   DAOQUC010000090 DAOQUC010000000
VERSION     DAOQUC010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4927
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18981_length_4927_cov_2.897989"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1000)
                     /locus_tag="PKH07_02475"
     CDS             complement(<1..1000)
                     /locus_tag="PKH07_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HNT33843.1"
                     /translation="MSRADLRFRQIHMDFHTSPDIDGIGDDFDPKDFAETLERARVDS
                     VTCFARCHHGYMYYNTRKFPGRRHPHLKRNLLEQQIEACHKRDIRVPIYITVQWDHFT
                     AQEHPEWLVLNEAGAPKGTPIFEAGFYRTLCVNSPYLDFLKSHVQEVLEVLPVDGLFF
                     DIVGPQDCSCRYCRDKMIAQGLAPTDASLRKRFGLDTINTFKLEMTKFVRQFNGDCTI
                     FYNAGHIAPRHRGVVGAYTHFELESLPSGGWGYSHFPITMGFARNLGLDCLGMTGKFH
                     TSWGDFHSFKNQEALEFECFRMLALNAKCSVGDQLHPSGKICQATYDLIGSVYGEVE"
     gene            complement(1201..1551)
                     /locus_tag="PKH07_02480"
     CDS             complement(1201..1551)
                     /locus_tag="PKH07_02480"
                     /inference="COORDINATES: protein motif:HMM:NF024833.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HNT33844.1"
                     /translation="MKRYSLVILGAVLMISAIGCNTTQKGAAIGAATGGVVGGVIGHQ
                     TGHTTGGAVIGAGVGAVTGGVIGHQIDKKNERESGNSSVSQEQQVQQPERTETQAAGE
                     VTDATVTQPQTETQ"
     gene            complement(1585..2310)
                     /gene="hisA"
                     /locus_tag="PKH07_02485"
     CDS             complement(1585..2310)
                     /gene="hisA"
                     /locus_tag="PKH07_02485"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008309558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HNT33845.1"
                     /translation="MLEIIPAIDLIGGECVRLLRGDYSQKTVYSRDTVAIAQKWQDLG
                     AQRLHVVDLDGAKAGVPKNLPVVLEIVKRTSLRVEMGGGLRDRDTIRSVMDAGVHYAI
                     LGTAALQNQLLLEGVASEYRERILVGIDARDGKVAVRGWLDVSEVTALDLIHLVNSLP
                     IGGVIYTDIARDGAMVGPNVESLRTVADESRHPVTASGGVTTVADVRNIADLKHPKVV
                     AAIIGKALYEQTIDLREAIRAAS"
     gene            complement(2324..2998)
                     /gene="hisH"
                     /locus_tag="PKH07_02490"
     CDS             complement(2324..2998)
                     /gene="hisH"
                     /locus_tag="PKH07_02490"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012673838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HNT33846.1"
                     /translation="MVHLIDYGMGNLHSVSKAFEKAGASVLISDNPNEISRAEALVLP
                     GVGAFRAAMCELQERSLIEPIMAHVRLGKPFIGICLGYQLLFESSEEDGPVKGLGIFK
                     GEVVRFQNHALEGDETNPTDEGQISHLTSVPLKIPHMGWNQVRQVQRSELFECIPDDA
                     FFYFVHSYYPVPRDPNVVIGETEYGVSFASVVGKDRIYGVQFHPEKSQTIGARMLSNF
                     VRIAFG"
     gene            3258..4385
                     /locus_tag="PKH07_02495"
     CDS             3258..4385
                     /locus_tag="PKH07_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HNT33847.1"
                     /translation="MSQSFHSICRWTFNAGKGGFVPSDIRPAWFSDGFTTADMIRLVK
                     KEIAPRMPKNVALGIELHYDSEVSEDNVKEVRDALGETGLWLAMITPGAHSHFAYGGI
                     SSLDPVERKAAEDLGRRTVDLAYGLLKKVWCPGKPPAFVLWNGSFGYDLASIGIRQMY
                     DNLKESVAGLCAYEQDRGGKLFICFEPKPNEGHPAMLVPTVASAILFWRKLEDQYGIS
                     RKRKGVNKEFGHSEMIGLDHVYDTVEELDNDAMVHMHLNSQGYNDGIILGGPGKFDID
                     HGTRINGMNVAIAGLIQDAGYDRWKGHDMQARSYDNERQGIDRVIRSVLSWEACERAA
                     KSLNQKQLMNLLAKRETAKAEDMLRQATAKAGQFFDQMYKG"
     gene            complement(4382..>4927)
                     /locus_tag="PKH07_02500"
     CDS             complement(4382..>4927)
                     /locus_tag="PKH07_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008689481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HNT33848.1"
                     /translation="GAIMGSGGLVVLDESDCMVDIARYFMEFTQRESCGKCTFCRIGS
                     KRMLEILVRLCSGEASQGDLEELESLGNLLKKASFCGLGKTAPNPVLTSLRYFREEYE
                     AHLNKHCPAGRCRALIQYEISEECIGCTICAQHCPVAAIEPTPYQCHSIKSELCVRCD
                     TCRTVCPQKAVKVVSGPYRIG"
BASE COUNT         1227 a         1390 c         1193 g         1117 t
ORIGIN      
        1 tctcaacttc cccgtataca gaaccaatga ggtcgtaggt tgcctgacaa atcttccccg
       61 aagggtgaag ttgatctccc actgaacact ttgcgttcaa agcgagcata cggaaacact
      121 caaattcgag agcttcctga ttcttgaaag agtggaaatc tccccaagaa gtatgaaact
      181 tgccagtcat cccaaggcag tccagaccga gattccgtgc aaatcccatg gtgatgggga
      241 aatgtgaata gccccaacca ccactgggaa gtgactcgag ttcaaaatgc gtataggctc
      301 caacaacacc acggtgccga ggcgcgatgt gtcccgcgtt gtagaaaatg gtgcagtccc
      361 cgttgaactg gcgaacaaac ttggtcatct ccaacttgaa tgtattgatg gtatcaaggc
      421 caaacctctt gcggagcgat gcatccgtcg gtgcgagtcc ctgcgcgatc atcttgtcgc
      481 gacaatatct acaggaacag tcctgagggc cgacaatatc aaagaaaaga ccgtcaacag
      541 gaagaacctc caagacctct tgaacatggc tcttcaggaa atccaaatac ggactattga
      601 cgcagagagt cctgtaaaag cctgcctcaa aaatgggagt tcccttgggg gcgcctgctt
      661 cgttgagtac cagccattcg ggatgctcct gcgcggtaaa atgatcccat tgaacagtaa
      721 tatagattgg aacccgaata tctctcttgt gacaggcttc gatttgctgt tctagcaggt
      781 tcctcttgag atgaggatgc ctgcgcccgg gaaacttgcg tgtgttgtag tacatgtagc
      841 cgtggtgaca ccgggcaaaa caggtgaccg aatccacgcg agcacgctcc agagtctcgg
      901 caaagtcttt ggggtcgaag tcatcgccaa tgccatcaat atccggactg gtgtgaaagt
      961 ccatgtgaat ttgtcgaaat cgcaggtctg ctcggctcat acaactatcc tccgctcccc
     1021 gatgacgggg ctttccaatg atgaatacaa cgtggattca ttgtttactg caaacccaac
     1081 tgctgtgcaa gggaaacttg atggatttgg ccagcaggca gtgtgagaca aaagagagac
     1141 tcccgggcca gtcctccatg aacaaggccg gggagtctca aggatggggt tgacggtatt
     1201 ctactgtgtt tcggtctgcg gctgagtcac agttgcatcc gtgacctcgc ctgccgcttg
     1261 agtctcggtt ctttccggtt gctgaacttg ttgttcttga cttacgctgg aattcccgct
     1321 ctcacgctca ttcttcttgt cgatctgatg accaataaca ccaccggtca cggctccgac
     1381 ccctgctcca ataaccgctc caccggttgt gtggcctgtc tgatgaccaa tcaccccgcc
     1441 aacaacgcca cccgtggcag cgccgattgc ggcacccttc tgggtggtat tgcacccaat
     1501 cgcggaaatc atcaaaactg ctccgagaat caccaaagaa tagcgtttca tccttctgta
     1561 cctctctttt ccagacggat cttcctagga agccgccctt attgcctcac gcaagtctat
     1621 cgtctgttcg tataacgctt tgccaatgat ggcagcaacg actttcgggt gcttgagatc
     1681 ggctatgttg cggacatcag ccaccgtcgt cactcctcca gaggcagtca cgggatgtct
     1741 tgattcatca gccacagttc gcagactctc aacattcggt ccgaccatgg ctccgtcccg
     1801 tgcaatgtca gtatatatga cgccgccaat cggaagacta ttcaccagat gaatcaaatc
     1861 cagtgcagtc acttcagata catccagcca tccccgaacg gcaaccttgc catctcgagc
     1921 gtctatccca accagaatcc tctcacggta ctcgctcgcc accccttcga gaagcagttg
     1981 attctgaaga gccgccgttc ccagaatggc ataatgaact cccgcgtcca taacgcttct
     2041 aatggtgtcc ctatcccgca gtccgcctcc catctcaacc cgcaaactcg ttcgcttcac
     2101 aatttcgaga acgacaggaa gattcttggg aactcccgcc tttgccccgt ccaaatcaac
     2161 cacgtgaagc ctctgcgctc ccaaatcctg ccacttctgt gcaatggcga cagtatccct
     2221 ggaataaaca gtcttctgtg aatagtctcc acgaagtagt cgaacgcact ctccaccaat
     2281 gagatcaatc gccgggataa tttctagcac tgacaaacct ctctcagcca aaggcgatcc
     2341 gaacgaaatt gctgagcatt ctggctccaa tggtctggct cttctccggg tggaactgaa
     2401 cgccgtatat acgatccttc cccaccaccg atgcaaaaga cactccgtat tccgtttcgc
     2461 caatcaccac attcggatcc cttggcacgg gatagtaaga atgcacgaag tagaagaacg
     2521 catcatcagg gatgcattcg aacaactccg accgctgaac ctgtctgacc tgattccaac
     2581 ccatgtgagg aatcttgaga ggcacagagg tcagatgtga aatctgcccc tcatccgtcg
     2641 gattcgtttc atcgccttcc aaagcatgat tctgaaagcg aacgacctca cccttgaaga
     2701 tccccagtcc tttcaccggg ccgtcttcct cgctggattc gaacagaagt tgatagccaa
     2761 gacagatgcc gataaacggt ttacctagac gcacatgtgc cataatgggc tcaataaggg
     2821 aacgttcctg caactcgcac atcgcagccc tgaacgcacc aactccaggc aagacaagcg
     2881 cttccgctct tgatatctca ttcgggttat ctgaaatgag cacactggca cccgccttct
     2941 caaatgcctt ggagacgctg tgcaaattcc ccattccata gtcaatgagg tgaacaattc
     3001 gttctggcaa ttcttcctct ccaactccat cgctcttgag aacacagact cctatgcccg
     3061 gagtgtaccg gattcgcact gggttcagat gatattgcag acgcgcttgc gattcaaccg
     3121 atttcaccga gagcggactt gaacctgact tgactttgcc gagccgggat tgatagattc
     3181 ccaaaagagt ttcgaaatcc aaatgaatct gaggagactg aaaacgacag tcattcccct
     3241 atggaggcac acccacaatg agtcaatcgt ttcatagcat ttgtcgttgg acgttcaatg
     3301 caggcaaggg aggctttgtt ccttcggata tcagacccgc atggttctca gacgggttta
     3361 ctaccgcgga catgattcgc ctcgtcaaga aggagatcgc gccccgaatg cccaagaacg
     3421 tggccttggg aatcgaactg cattacgact ccgaggtcag tgaggacaac gtcaaggaag
     3481 tccgagatgc ccttggcgag accggtctct ggcttgccat gatcaccccg ggcgctcaca
     3541 gccactttgc ctacggtgga atctcttctc ttgatcctgt cgaacgcaaa gcggcggaag
     3601 accttggtcg aaggacggta gatttggcct atggtctctt gaagaaagtc tggtgtcccg
     3661 gaaaaccacc agcattcgtt ctctggaacg gctcattcgg atatgacctc gcgtccatcg
     3721 gaattcgtca gatgtatgat aacctcaaag agagtgttgc cgggctttgc gcctacgagc
     3781 aggatagggg tggcaaactc ttcatctgct tcgagccaaa acccaacgag ggacatcctg
     3841 cgatgctcgt tccgaccgtg gccagcgcca tcctcttctg gaggaaactc gaggatcaat
     3901 acggaatctc cagaaagcgc aagggtgtca acaaggagtt cggtcactcg gagatgatcg
     3961 gtttggatca tgtctatgac accgtcgagg aattggacaa cgacgccatg gtgcatatgc
     4021 acctcaatag ccagggatac aatgatggca tcatcctggg tgggcctggg aaatttgaca
     4081 ttgaccatgg cacccgaatc aacgggatga acgttgccat cgcggggctg attcaggatg
     4141 ccggctacga ccgctggaaa ggtcatgaca tgcaggctcg ctcctatgac aacgaacgcc
     4201 agggcattga tcgcgttatc cgcagcgtct tgagttggga ggcctgcgag cgagcagcaa
     4261 agtcgctgaa ccagaaacag ttgatgaacc tacttgccaa gcgcgaaaca gccaaagccg
     4321 aggacatgct gagacaggca acagccaagg caggccaatt cttcgatcaa atgtacaagg
     4381 gttagccaat tctgtacgga ccagaaacga ccttcacggc tttctgtgga cacacggttc
     4441 gacatgtgtc gcaacggacg cacagttcac tcttgatcga gtgacattgg tacggggtgg
     4501 gttctattgc agcaactgga caatgctgtg cgcagatcgt acacccaatg cactcctcag
     4561 aaatctcgta ttggatcagt gcacgacatc gtccggccgg gcaatgctta ttgagatggg
     4621 cttcgtattc ctctctgaaa tacctgagcg acgtcagaac aggattgggc gctgtcttcc
     4681 ccaaaccgca gaaactcgct ttcttaagga gattcccaag actctccagt tcttccaagt
     4741 caccctgact cgcttctcca ctacagaggc gaacaagaat ctccaacatc cgctttgaac
     4801 cgattcggca gaaggtgcac ttgccgcagg actctcgctg agtgaactcc atgaagtagc
     4861 gggcgatgtc caccatgcag tctgactcat cgagcacgac aagaccgccc gatcccatga
     4921 tcgcccc
//
LOCUS       DAOQUC010000091         4924 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_18999_length_4924_cov_3.195933, whole genome shotgun sequence.
ACCESSION   DAOQUC010000091 DAOQUC010000000
VERSION     DAOQUC010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4924)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4924)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4924
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18999_length_4924_cov_3.195933"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..1201)
                     /gene="ftsZ"
                     /locus_tag="PKH07_02505"
     CDS             complement(2..1201)
                     /gene="ftsZ"
                     /locus_tag="PKH07_02505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HNT33849.1"
                     /translation="MVEEFEYKSGIELIEEEFEMNGEVTPRILIIGVGGAGCNVLDSM
                     YSVGLDNVSFLAVNTDMQSLGRTVCPDRLHIGRGVTDGLGTGGNPLLGERSAKEDRER
                     LSESLKGHDLVFLIAGLGGGTGTGATPVIAEIAHEQNSLTVAIVTKPFEFEGPVRRRN
                     AERGREEIRRRVDTLITMPNDRLVRMVDEKTTFTEALGEANRMLYDCVEGLTQLTTEW
                     GIVNLDFEDVRATMANNGGALMGIGQGQGEDKMHCALEEAMNNLSLDETHLDGLNNAL
                     INVTAGPDLTIHEVNTAIGELVQKHGGHDANIRFGLVIKEAMQGRVRVTLLATGHSEV
                     VQEMPVAPADSFTTRMSRTMETLEVPKRRVLGISFLDRAESNKGELFFKQDETEEELL
                     KVSGNNR"
     gene            complement(1264..2484)
                     /gene="ftsA"
                     /locus_tag="PKH07_02510"
     CDS             complement(1264..2484)
                     /gene="ftsA"
                     /locus_tag="PKH07_02510"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HNT33850.1"
                     /translation="MSTDSILAVLDIGSTEIRAVVGRVLEPGKVEVIGFGAVPSVGIS
                     RGSVVDIEANTRVIQKAVEAAREKAGVFPQRVIVGVNGEGLKTFGASGTVAIMSKDRG
                     ITKDDINRVLRNAAERNVPVEHAVLDRIPCTFQVDQTTGVQDPVGKTGDLLAAEVFLV
                     TCPRPVLADLERCIKGANLRVQKFVPTGLAASLAVLSDEEKNAGVALVDIGGGTTNVV
                     VYFDGYVIHAEVLTKGGEGITRSIARSLTCSMETAAFLKKTYGVAMPEMVTPVETVPV
                     KRLPPRQQKEIPRRVLSEIIEQGLDEILRDVGRVFHHRELTHRLFAGVILTGGGALLT
                     GMKEKVENTLKIETHIGIPSRLEGFEMVFRNPRFASVAGLLEYASRDEVQQQTRAPFI
                     FRLLERVAGLFGYL"
     gene            complement(2481..3398)
                     /locus_tag="PKH07_02515"
     CDS             complement(2481..3398)
                     /locus_tag="PKH07_02515"
                     /inference="COORDINATES: protein motif:HMM:NF015739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HNT33851.1"
                     /translation="MRRPAGRRTRLEEPDVQSGFWQGLAKAMKILLRITVVLGTCFLL
                     YQLYWLMVYSEFFRIERVEIYGCKQCTPEGIQARLPWKEGDSLWTINLKKSVECVLAE
                     PWVKRVTVRRVLPRRVEIHLTERTPIAAIPDDQGSRYFGLDERGVVLPVVAVVRGAPH
                     LLNRDSSPRLPIITGLSPSEVQPGNQIAEEKISPVIELISVLGQSQPTLAAVLFEFNI
                     STPDSIVGYPSIRVKKVFFGAENFPQRVSRLAEVWKYLDREGMHGESIDLQFDDQGVV
                     FSPSQTQVPDRVQQDDAGHLLQTDGGRNG"
     gene            complement(3391..4785)
                     /gene="murC"
                     /locus_tag="PKH07_02520"
     CDS             complement(3391..4785)
                     /gene="murC"
                     /locus_tag="PKH07_02520"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HNT33852.1"
                     /translation="MTVQHNDIIHFIGVGGIGMSGIAQVLASKGYPVSGSDLKTTSIT
                     RRLSALGVRICEGHKAENVKDATVVVYSSAVRESNPELQEARKRGLKIMRRAEMLAFV
                     LGWGRSIVISGAHGKTTTTSMVVTTLEAAGMNPTSLIGGELNDIGGNARLGTGEWAVA
                     EGDESDGSLVLLPADVAVVTNIDLEHLDHYPDIASVKACFRQFFKSAKPGARYIVCAD
                     SPVALELASEFPRESVLLYGRGENAQYQIRDLEARHDATEFSLTCEANLMGRVRVNVP
                     GVHNASNATAACLAAMQAGLDFEAAADGLARYRGVRRRFEFKGTVGGVTVVDDYGHHP
                     TEITATLATLSGYTQGRRVVIFQPHRYTRTAYLMKDFCTSFQQAKLDWLIVTEVYSAG
                     EDPIPQATGEHLAHEIQKGASFKVSYQPRVPDIAREIVAHINSGDTVLTLGAGDVVKA
                     GETLLSILGGSGNA"
BASE COUNT         1058 a         1503 c         1237 g         1126 t
ORIGIN      
        1 cctatctgtt gttgcctgag actttcagga gttcctcttc cgtctcgtcc tgcttgaaga
       61 agagttcgcc cttgttggat tccgctctgt ccaggaaaga gatgcccagg actcggcgtt
      121 ttggaacctc caacgtttcc atcgttctgg acatccgagt ggtgaaggaa tctgccgggg
      181 cgactggcat ctcctgaacc acttcagaat gccccgtggc cagcagtgtc acgcgaacgc
      241 gcccctgcat ggcttccttg atgaccagtc cgaatcgaat gttcgcatcg tgaccaccgt
      301 gtttctgaac cagttctccg atggcagtat tgacttcgtg gatcgtgaga tcggggcctg
      361 ccgtgacgtt gatcaatgcg ttgttcaaac cgtcgagatg cgtctcatcc agggagaggt
      421 tgttcatggc ctcttccaga gcgcagtgca tcttgtcttc gccctgaccc tgaccgatgc
      481 ccatcagggc accgccgttg ttggccatgg tggctcgaac atcttcaaag tcaaggttca
      541 caatacccca ttccgtggtc agctgggtga gaccttccac acagtcatag agcatccgat
      601 ttgcctctcc cagcgcttca gtaaacgtgg tcttttcatc caccatgcgt acgagacggt
      661 cgttgggcat ggtgatcaat gtgtccactc gacgtcggat ttcctcgcgg ccgcgctctg
      721 catttcggcg gcgcaccgga ccttcaaatt cgaagggctt tgtcacaatg gccacagtga
      781 gcgaattctg ctcgtgcgca atctccgcga tcacaggagt tgcacccgtg cccgttccac
      841 caccaagacc ggcaatcaga aacacaaggt catgaccttt gagcgattca gaaagccgtt
      901 cccggtcttc ctttgcggat cgctcgccga gcaacggatt gcctcccgtg cccaggccgt
      961 ctgttacgcc tctgccgatg tgcaggcgat cgggacacac cgtgcgcccc agggactgca
     1021 tatccgtatt gacagccagg aacgaaacgt tgtccaaacc aacgctgtac atgctgtcca
     1081 ggacattgca tcctgcacca ccgacaccga tgattaagat gcgcggtgtc acttctccat
     1141 tcatttcaaa ctcctcttcg ataagctcga ttcccgactt gtactcaaac tcctcgacca
     1201 tttgaccccg tcctttccgc atggcttccc caaaggatct ctcctttgac catgcctctg
     1261 ctgctaaaga tacccgaaca aacctgccac ccgctccaga aggcggaaga tgaatggagc
     1321 acgcgtctgc tgctgaacct catcccggga cgcgtattcc aagagtcccg ccaccgaggc
     1381 gaacctcgga ttgcggaaca ccatctcgaa gccctcaagg cgcgagggaa tcccaatatg
     1441 tgtctctatc ttcaaagtat tttcgacttt ctccttcatg cccgtgagca acgcaccacc
     1501 gcctgtgaga atgactccgg cgaaaagcct gtgagtcaat tctctgtggt ggaagacacg
     1561 acccacgtct ctgaggatct cgtcgagtcc ctgctcgatg atctcggaca agacgcgtcg
     1621 tggaatctcc ttctgctgcc gtgggggaag tcgcttcacg ggaactgttt cgaccggtgt
     1681 gaccatctca ggcatcgcca cgccataggt tttcttcagg aaggcggccg tctccatgga
     1741 acaggtcaga ctgcgcgcga tagaacgggt gataccctca ccacccttgg taagtacttc
     1801 agcgtggatg acgtatccat caaagtagac gacgacattt gtcgttcccc cgccgatatc
     1861 aacgagcgcc accccagcgt tcttctcctc atcggacaag accgcgaggc ttgcagcaag
     1921 accggttggc acgaacttct gcacacgcag attcgcaccc ttgatgcagc gttccagatc
     1981 tgccaacact gggcgtggac aggtgacgag aaacacttcc gcggccagaa gatcgccggt
     2041 tttgccaacg ggatcctgga ctcctgttgt ctgatctact tgaaaggtgc agggaatccg
     2101 atccagaaca gcgtgctcca cgggaacatt tctctccgcc gcgttacgca ggacgcggtt
     2161 gatatcgtcc tttgtgattc cccgatcctt gctcatgatg gcaacagttc cagaggctcc
     2221 aaaggtcttc agtccttcgc cgttgactcc aacgatgacg cgctgtggga agactccggc
     2281 cttctcgcgt gccgcctcta ccgctttctg gatcacgcgc gtgtttgcct ctatgtccac
     2341 caccgatccg cgcgagatac ctacggacgg aacggcacca aagccgatca cctcaacctt
     2401 gccaggttcc agaactcttc cgacaacagc cctgatctct gtcgatccaa tgtccagaac
     2461 ggccagaata ctgtcagtgc tcatccgttg cgtcctccgt cggtttgcag cagatgaccc
     2521 gcgtcgtcct gctgcacacg atccggtact tgtgtttggc ttggggagaa gacgactccc
     2581 tgatcgtcaa attgcagatc aatagactct ccgtgcatcc cctccctgtc caggtacttc
     2641 cagacctcgg caagtcggga gactctctgg ggaaaattct ctgcgccaaa gaaaaccttc
     2701 ttcacccgaa tactcggata tccgacaatg ctgtcaggtg ttgaaatgtt gaattcgaac
     2761 agtacggcag ccaatgtcgg ctgactctgc ccaagtactg agatcaactc aatcactggg
     2821 ctaatcttct cttccgcaat ctggtttcct ggttgcactt ccgacggact gagaccagtg
     2881 attatcggca gacgcggact gctgtctcgg ttcagaagat gcggagcacc gcgcaccacg
     2941 gccaccacgg gaagaaccac tcctctttca tccagaccga aataccggct tccctgatca
     3001 tcgggaatcg ccgcgatggg agtgcgttcg gtcagatgta tttccacccg acgaggcaag
     3061 acacgtcgaa ccgttacgcg cttgacccag ggttcggcaa gaacacactc aacagacttc
     3121 ttgaggttaa tcgtccagag actatcgcct tccttccagg gaagccgtgc ctgaattcct
     3181 tccggtgtac actgcttgca gccgtaaatc tcaacccgtt cgatgcggaa gaactccgaa
     3241 tagaccatga gccagtaaag ctgataaagc aaaaagcacg tccccagaac cacagtgatt
     3301 cggagcaata tcttcattgc tttggcgagt ccttgccaga agcccgactg cacgtcgggt
     3361 tcctcaagtc ttgtacgtcg tcctgctggc ctacgcattc ccactgcctc ccaggatact
     3421 cagtagcgtt tcgcccgctt tcaccacatc ccccgcacca agggtaagca cggtgtctcc
     3481 cgagttgata tgagcgacaa tctcacgggc gatatcggga acccgtggtt ggtaagagac
     3541 tttgaaggac gcacctttct gaatctcatg agccaagtgc tctccggtgg cctgaggaat
     3601 cggatcctca ccggcggagt agacctccgt gacgatcagc cagtccagct ttgcttgctg
     3661 gaaacttgta cagaagtcct tcatgagata cgccgttcgc gtgtatcggt gaggctggaa
     3721 gatgacaacg cgcctgcctt gcgtataacc actcagcgtg gcaagagtgg cagtaatctc
     3781 ggtgggatga tgcccgtaat catcaacgac ggtgactccg ccgaccgttc ccttgaactc
     3841 aaatcgccga cgaacgccgc gataacgggc aaggccgtcc gccgctgcct caaaatccaa
     3901 gccagcctgc atcgcagcaa gacatgccgc ggtggcattg gaagcattat ggactccagg
     3961 gacattgacc cgcactcttc ccatgaggtt cgcctcgcag gtcagggaga actccgttgc
     4021 atcgtgtctg gcttccagat cgcgaatctg gtactgggca ttctctcctc gcccatagag
     4081 caggacagac tcacggggaa actccgatgc caactccaac gcgacaggac tgtcagcaca
     4141 aacgatatag cgtgctcccg gtttcgcact cttgaagaac tgccgaaaac aggctttgac
     4201 actggcaatg tcaggatagt gatccaaatg ctccaaatct atattggtca cgaccgcaac
     4261 gtcagcgggc agaagcacca gagagccatc gctctcatcg ccttctgcga ccgcccattc
     4321 accggtaccc aaccgtgcgt ttccgccgat gtcgttcagc tctccgccaa tcaaggaagt
     4381 gggattcatt cccgcggctt ccagcgtcgt cacaaccatg gatgtcgttg tcgttttgcc
     4441 atgtgctccg ctgatgacga tcgagcgtcc ccaccccagc acgaaagcca gcatctcagc
     4501 gcgacgcatg atcttgagac cacgcttccg tgcttcctgg agttcgggat tgctttcccg
     4561 cactgctgac gagtagacca caacggttgc gtccttgacg ttctccgctt tgtgaccttc
     4621 acagattctc acacccagcg ctgaaagcct tcttgtgata gaggtggtct tgagatcgga
     4681 accggatacg ggatagccct tcgatgcaag aacctgagcg attccgctca taccaattcc
     4741 accgacaccg atgaagtgga ttatgtcgtt gtgttgcact gtcatcgttg tgcgaccatt
     4801 gcctccagtt cggtaagcat tgctgctgtg gcattgggct ttcccaagga gcgtgcagcc
     4861 tcggctcttt gtgataactt cacagggtca tccatgagtg agatcacttc ttccgcgaga
     4921 cttt
//
LOCUS       DAOQUC010000092         4920 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_19022_length_4920_cov_3.346968, whole genome shotgun sequence.
ACCESSION   DAOQUC010000092 DAOQUC010000000
VERSION     DAOQUC010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4920
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19022_length_4920_cov_3.346968"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..429
                     /locus_tag="PKH07_02525"
     CDS             <1..429
                     /locus_tag="PKH07_02525"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HNT33853.1"
                     /translation="VGKIALGTFVEEYLTRMQMYALENKVPFEEAERTIIGMDHAEAG
                     AILLEQWSLPSCMINVVRWHQEPDRAPVQDLVIDLVHAADALMLSGGVGAGNDGLNYR
                     LSRSVVSRLGVTIHVAEAVVCMTMSAVEELKDSFLGGSGR"
     gene            436..822
                     /locus_tag="PKH07_02530"
     CDS             436..822
                     /locus_tag="PKH07_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNT33854.1"
                     /translation="MGFNVLIVDDSVTVRAVIVKTLSVAEVPVNEVFQAANGKEALDI
                     LANSWVDLVFADINMPVMTGIELVDRMAADGLLQTIPVVIVSTEGSKTRIEDLKGKGV
                     SAYIRKPFTPELLKQVVDMVMGSQDE"
     gene            815..1279
                     /locus_tag="PKH07_02535"
     CDS             815..1279
                     /locus_tag="PKH07_02535"
                     /inference="COORDINATES: protein motif:HMM:NF025070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HNT33855.1"
                     /translation="MNEEMTETLNRIVCDVFERMAFMFGEIVEKASLPSLQRTGYLAK
                     MGFEGPICGSVSMAVAQSMCPVIAANVLGMDEDDEFVTEKSLDALKEMLNVICGQIVT
                     SLAGDEPVFDLSVPQVVDIGADEWKSLMDQEESLAFIVDDEPVLFHLTLESR"
     gene            1298..2347
                     /locus_tag="PKH07_02540"
     CDS             1298..2347
                     /locus_tag="PKH07_02540"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013163898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HNT33856.1"
                     /translation="MNEMLSSPRKIRVLIVDDSAVVRAIFSSELSSDPTLDVVGTAPD
                     PYVARDLIVQKKPDVVVLDIEMPRMDGVTFLRKLMKYYPLPVIIVSSLTPKGGDLAIE
                     ALEAGAVEVMCKPGTAYTVGDMAVQLREKIRAASFVRVQKRDPSLQKQRVAAPTLAMT
                     RTTNKVVAMGASTGGTQALEAVLTKLPADCPGIVIVQHMPEHFTRSFAERLNTYCPME
                     VKEAENNDSVVPGRALIAPGNYHMLLRRSGARYYVEVKSGPLVNRHRPSVDVLFKSVA
                     RYAGRNAVGVIMTGMGNDGAQGLLEVRNAGAETIAQDEASSVVFGMPKEAIELGAASH
                     IRSLDFIPAKILELV"
     gene            complement(2356..2616)
                     /locus_tag="PKH07_02545"
     CDS             complement(2356..2616)
                     /locus_tag="PKH07_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33857.1"
                     /translation="MKDHIILGVHITNRMKNAVRVQEILTEYGCHIRTRLGLHEVDEN
                     FCAQGGVLILEMVGEDKTIDEMAAKLKAVEGLELQKLVFRHN"
     gene            complement(2657..4063)
                     /gene="hydG"
                     /locus_tag="PKH07_02550"
     CDS             complement(2657..4063)
                     /gene="hydG"
                     /locus_tag="PKH07_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012625131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HNT33858.1"
                     /translation="MNDAVQTTIIDEGKIQETLFRTRSLSDDSGHIKEVLAKALELKG
                     LSAEETALLMNVSDPTLERAISDTAKRIKEQIYGKRLVLFAPLYISNLCQNECLYCAF
                     RAKNKAVKRRSLDQEEIAREVVTLVNQGHKRLLVVVGEAYPKEGFSYVTRSIETVYSV
                     KTDKGEIRRVNVNVAPLTLDEFRMLKATGIGTYQLFQETYHRETYQRVHLSGKKRDYD
                     WRISATDRAMTAGIDDVGVGVLFGLYDWRFEILAILQHVAHLEKTFGVGPHTISVPRL
                     EPATGSEMSEHPPFPVSDEDFRKIIAILRISVPYTGIILSTRESPETRRESFALGVSQ
                     ISAGSRTNPGGYSEDDRDPDSSQFSLGDHRTLDEVIRDVASLGYIPSFCTGCYRLGRT
                     GQDFMDLAKPGDIKYHCDPNALSTFTEYLLDYASEETRQAGEALIEKTLAQMDDKQRE
                     ISLGLVQQVRAGKRDVFC"
     gene            complement(4180..>4920)
                     /locus_tag="PKH07_02555"
     CDS             complement(4180..>4920)
                     /locus_tag="PKH07_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HNT33859.1"
                     /translation="KKFECNRPEMCDSGYKDIDYGLTTRELGQMISEAGVDLPNVTKS
                     GFDDPFGPPTGSGIIFGATGGVMEAAIRTVIELVCGIKIEKLFEHADIIPLRGFDGIK
                     YAEITLPEIGPVPDILKPLVKDWEWLRGATLKAAICHGTANAKKVLEDVKHGGKLSEC
                     HFIEFMACPGGCLGGGGQPIPTSPAIREARARAIYAEDAAYEIRKSHENPAVVELYTK
                     FLTDGPCGHLSHKLLHTHYTPRGKFIAE"
BASE COUNT         1115 a         1206 c         1353 g         1246 t
ORIGIN      
        1 gttgggaaga ttgctctcgg tacatttgtt gaggagtacc tgactcgcat gcagatgtat
       61 gccttggaaa acaaggttcc atttgaagag gcggagcgca ctattattgg aatggatcac
      121 gcagaggcag gtgctattct attggaacag tggagtctgc cgtcctgtat gatcaacgtg
      181 gttcgatggc accaagaacc ggatcgggct ccagttcagg atttggtgat tgatctggtc
      241 catgcggcgg acgctctcat gctcagtgga ggggttgggg caggtaatga tggtctgaat
      301 taccgtttat cccgttccgt ggtatcgagg ctcggggtca ccatccatgt tgctgaggcg
      361 gtggtctgta tgacgatgag cgcggtggaa gagttgaagg acagtttcct tggtggctca
      421 gggaggtagg tagaagtggg tttcaacgtt ctaattgtgg atgactcagt cactgttcgt
      481 gccgtcatcg tcaagacttt gagtgtggct gaagtgccgg tcaacgaggt atttcaggcg
      541 gccaatggca aggaagcgct tgatatcctc gccaattcct gggttgacct tgtttttgcc
      601 gacatcaata tgcctgtgat gacggggata gaattggtgg atcgaatggc cgcggatggg
      661 cttttgcaga cgattccagt agtcattgtt tccacagagg gcagcaaaac acgaattgag
      721 gatttgaagg ggaagggcgt cagtgcctac attcgtaagc cattcacgcc ggaactgctt
      781 aagcaggttg tggatatggt aatggggagt caagatgaat gaggaaatga cagaaactct
      841 gaaccggatc gtctgtgacg ttttcgagag gatggctttc atgtttggtg agatcgtcga
      901 gaaggccagt ctgccgagtc tgcagcgaac cggttacttg gcaaagatgg gctttgaggg
      961 tccaatctgc ggctctgtca gcatggcagt ggcgcagtcc atgtgccctg tcattgctgc
     1021 caatgttctg ggaatggatg aagacgacga gttcgtcact gagaagtcac tcgacgctct
     1081 caaggaaatg ctcaatgtca tctgcggtca gatcgtcacc tctctggctg gtgacgaacc
     1141 tgtgtttgat ctgtcagttc ctcaggttgt agatattggt gctgacgagt ggaagagcct
     1201 gatggatcaa gaagagtcac ttgcattcat tgttgacgat gagccggtgc tatttcacct
     1261 taccttggag agtcgctgag gaagacatat tgatgcgatg aatgaaatgc tttcgagtcc
     1321 acgcaagatc cgtgttctta ttgttgacga ttccgcagtt gttcgagcca tattctcctc
     1381 agagttgtcc agtgatccca cgcttgatgt ggttggcacg gcacccgatc cctatgtagc
     1441 gcgagacctg attgtacaga agaagccgga tgtcgtggtt ctggacattg agatgcctcg
     1501 gatggatggt gtcacgtttc tgcgcaagtt gatgaagtac tatccgctgc ctgtaatcat
     1561 tgtttcttcc ttgactccca aaggtggcga tttggccatt gaggctcttg aggccggtgc
     1621 ggttgaggtc atgtgtaagc ctgggactgc ctatacggtt ggcgacatgg cggtacaact
     1681 tcgtgagaag attcgggcgg cctcctttgt ccgagttcag aaaagggatc cttctttgca
     1741 gaaacaacgc gtggcggctc ccacgcttgc aatgacgcga acaacaaata aggttgttgc
     1801 catgggagct tccacaggag ggacacaggc attggaagcc gttctgacga agcttccagc
     1861 tgactgtcca ggcatcgtga ttgtccagca catgccagag catttcactc ggtcgtttgc
     1921 cgaacgcctg aacacctatt gtcccatgga ggtcaaggaa gctgagaaca atgacagtgt
     1981 ggtgccaggt cgtgccctta tcgctcccgg aaactatcac atgcttcttc gaagatcggg
     2041 cgcgcgttac tatgttgaag tcaagagtgg tcccttggta aatcggcatc gtccttcggt
     2101 ggatgtgctt ttcaaatctg tcgcgagata tgcgggtcga aatgccgtcg gagtcatcat
     2161 gacaggtatg gggaacgatg gagctcaagg attgctcgaa gtcagaaatg ccggtgccga
     2221 gacgattgca caagacgaag catcctctgt tgtttttggg atgcccaagg aagctataga
     2281 actcggtgca gccagccaca tccgttcgct ggatttcata ccagccaaga ttctcgagct
     2341 tgtctgaacg ttcgatcagt tgtgtcggaa gacgagtttc tggagttcaa gtccttcaac
     2401 agccttcagc ttcgcggcca tctcatcaat tgtcttgtcc tcaccgacca tctccagaat
     2461 caagactcca ccttgagcgc agaaattctc gtccacttca tgcagaccga gccgagttcg
     2521 gatatggcat ccgtattcgg tgagaatctc ctggactcga accgcgttct tcatgcggtt
     2581 ggtgatgtgg actcccagaa taatgtgatc cttcattgtg gcactccctt aacgaaaata
     2641 tagccgtgtc tgtccttcaa cagaacacat ctctttttcc tgctcgcact tgttgcacaa
     2701 gtccgagtga aatctccctt tgcttgtcat ccatctgtgc gagggtcttc tcaataagag
     2761 cctctcctgc ttgtcgtgtt tcctcggaag cgtagtcaag cagatactca gtaaaggttg
     2821 acagagcatt gggatcacaa tggtatttga tgtctccggg cttggccaga tccatgaagt
     2881 cctgccccgt tctgccgagc cgatagcagc ccgtgcaaaa cgacgggata tagccaagag
     2941 acgcaacgtc tcggatgact tcatccagtg tacggtgatc acctaacgaa aactggctgg
     3001 aatcggggtc gcgatcatct tcagagtagc cgccaggatt agtgcgactg cccgcagaga
     3061 tctgtgatac gcccaaggcg aaagactccc ttcgagtctc aggtgactcc cgagtcgaga
     3121 ggatgattcc cgtgtatggg acagatatcc gcaggatggc gataattttt cggaaatcct
     3181 cgtcagaaac agggaatggg gggtgttcag acatttccga accagttgca ggttccaatc
     3241 gaggtacact gatcgtatgg ggtccaactc cgaatgtctt ctcaagatgg gcgacatgtt
     3301 gaagtatcgc aagaatctca aaacgccagt cgtacagccc gaagagcacc cccacgccaa
     3361 cgtcatcaat gcctgcagtc atcgccctat ccgttgcgga gatccgccaa tcatagtcac
     3421 gcttcttgcc gctgagatgc actcgctgat aggtctcccg gtgataggtc tcctggaata
     3481 gctggtaggt tcctatgcct gtggctttca gcatgcggaa ctcgtcaaga gtcagagggg
     3541 ctacattcac atttacgcga cgtatctccc ctttgtcggt cttgacactg tagaccgtct
     3601 caatggatct agtgacgtag gagaagcctt ctttgggata ggcctcgccc acgacgacca
     3661 gaagcctctt atgcccctga tttaccaaag tgacgacctc acgggcaatc tcttcctggt
     3721 cgagagaacg acgcttgacc gctttgttct ttgctcggaa tgcacagtag aggcactcat
     3781 tctgacagag atttgagatg tacaatgggg cgaaaagaac aagcctcttg ccatatattt
     3841 gttccttgat acgttttgcg gtgtcagata tcgctcgctc taaagtggga tcgctgacgt
     3901 tcataagcag tgctgtctct tcagcactca accctttgag ctccagtgct ttggcaagaa
     3961 cttctttgat atgacctgag tcgtcggaca gagatcgggt tcggaaaagg gtttcctgga
     4021 ttttcccctc atcaatgata gttgtctgca cagcatcgtt catcgaaatg gcaacctcct
     4081 cgacatcacg tcacagaaga agcatacatc ataacgggag aaagcgaaac gccgaaagac
     4141 aaaagtgtct ttcggcgtct gaaaaccttg ctgagatcct cactcggcaa tgaactttcc
     4201 ccgcggtgtg tagtgtgtgt ggagcagttt gtgactgaga tgaccgcaag gtccgtccgt
     4261 caagaacttc gtgtagagct ccaccacagc gggattctca tgcgacttgc gaatctcata
     4321 ggcggcatct tcggcataga ttgcacgagc ccgtgcttct cgaatcgcgg gactggttgg
     4381 aatcggctgg ccaccgccgc cgaggcagcc accagggcat gccatgaact caatgaagtg
     4441 acactcagac agcttgcccc cgtgtttgac gtcttcaaga accttcttgg cgttcgccgt
     4501 tccgtggcag atagcggctt tcaacgtagc gcctctgagc cattcccagt ccttgaccaa
     4561 gggcttgaga atatccggta cggggccgat ttccggaagg gtaatctcgg cgtacttgat
     4621 cccgtcgaaa ccgcgcaagg ggatgatatc tgcgtgttca aaaagtttct cgatcttgat
     4681 tccacagacc agttcgatca cggtgcggat ggctgcctcc ataacaccac cggtggcacc
     4741 gaagatgatc cccgagcctg tgggtgggcc gaacgggtca tcaaagccgg atttcgtgac
     4801 gttgggaaga tccactcccg cctcactgat catctgaccc agttcgcgag tggtcagtcc
     4861 gtaatcaatg tctttgtagc cgctgtcaca catctcaggt cgattgcact caaacttctt
//
LOCUS       DAOQUC010000093         4904 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_19103_length_4904_cov_2.923077, whole genome shotgun sequence.
ACCESSION   DAOQUC010000093 DAOQUC010000000
VERSION     DAOQUC010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4904
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19103_length_4904_cov_2.923077"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..716)
                     /locus_tag="PKH07_02560"
     CDS             complement(<1..716)
                     /locus_tag="PKH07_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33860.1"
                     /translation="MRGMTEREIPVPSTPEQGLTDRIVTLRAVGIACGLMPLLSLWVF
                     QSELIWYSGHSTAISLFFHVTFTLLIIASLNLFVQKRWPSSALTSGEMMTIYVMLSVA
                     GTLCSHDLIQIFLPMLTFPKYNANPNNRWDTLIMPFIPSWAIVTDREAIVGLAVGNAS
                     LYSVRILLAWLKPLAFWSVFLLALIGALLFLNIFFRKAWTERERLSFPIIQIPMLITT
                     NLSSLFKSKLFWLAFGMVAS"
     gene            1002..1652
                     /locus_tag="PKH07_02565"
     CDS             1002..1652
                     /locus_tag="PKH07_02565"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNT33861.1"
                     /translation="MDRYCRENRGFTLIELLIVVAIIGILAAIAIPNFLLAQARAKVA
                     STHAGMQTITVGVEAYAVDNNVYPSSWLNYGQGAGWINLFTDRLKPITTPVAYIKRVP
                     ADPFVNKTQGQFFSGVKWSVDAYLAIQYAEDYFIPVSMRNAVSPPSRPGAGYVDGKGI
                     NVCYKLEGVGPDAIYNWMLPGRPANYETIIYDPTNGTVSYGDIRRYGGNDRGNIHY"
     gene            1857..2177
                     /gene="cutA"
                     /locus_tag="PKH07_02570"
     CDS             1857..2177
                     /gene="cutA"
                     /locus_tag="PKH07_02570"
                     /inference="COORDINATES: protein motif:HMM:NF015077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HNT33862.1"
                     /translation="MILVYITNPSCEQARTLAKHLIEKRLIACANIYSGVTSLYRWKG
                     TLAEESECVMIGKTQEALFDSVRREVERVHPYEIPCILKIPVSANEAFLNWLEGEVSQ
                     ETGL"
     gene            2303..3208
                     /locus_tag="PKH07_02575"
     CDS             2303..3208
                     /locus_tag="PKH07_02575"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HNT33863.1"
                     /translation="MNDSFYRMLMDNLSEGIYFVDTERKITYWNHGAERLTGFLSDEV
                     KGKLCRSNILMHVDNNGLSLCRSCCPAALTLNDGQPREIEAYLHHKEGHRVPVLMRLS
                     PIRNDVGEITGVMEVFSDNTVNLSARDQIEKLQVMALLDPLTEVGNRRFAEVCISARL
                     NELNRYGWKFGLLFIDVDGFRSTCEKYGHDISNRLLRMIARTIRNRLRCFDDVARWGE
                     DEFVAAVSNVDSESLSIVANTLRRLIEESGLIVETGLIQLTASIGATLAESVDSVGLL
                     VERSEQLIKQAKTDGRNRIATRLNR"
     gene            complement(3325..>4904)
                     /locus_tag="PKH07_02580"
     CDS             complement(3325..>4904)
                     /locus_tag="PKH07_02580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33864.1"
                     /translation="DALLAYVESQRAFFRSVSGAPSDAYHYAIFNKNTPTGGLLGLGY
                     YPSPRSAIGWDNRDDPNAPRGGMTMAHEIGHNHGRPHTWDEAAAPNSIREVGVDVYAY
                     RTWPTNTIACMNYGAPRWVSDYTWERLFDRRSSSPAPPLLEEGKRQVYVIAGVGRRDG
                     SGHLDPSYVIESGEPLDASNEAGPFFIQLRTDKDELLYSHPFDTSMEACYSTNATHIA
                     QTEGRSPFAFILPYSNKVGKIQLKLGEQLIDELVAAGVPMGVTVTYPNGGEVLTPSDK
                     IRWTATDIPDVTLTFQLFYSHNAGQTWEPMLGGLPAVVTPLQGYKVSRINGQFEMALD
                     SEAERMAGSGDMLIRVLASDGVRTVEDISDGTFTVESKPPIVTISSPFEGSKHPIGNT
                     VVFSGYAFDKEDQGAVPDEKLSWLWKPEAAGLVEFATGRDAFLLDIPEGDIKVWFVAE
                     DSDGQSATAEITINGDTPILGDLNNNWQLDGGDVMMYGAIWSATSGGENFYGIADIDA
                     DGIIDNKDVLLLLTNMN"
BASE COUNT         1177 a         1207 c         1343 g         1177 t
ORIGIN      
        1 atggacgcga ccatcccgaa agcaagccaa aacaacttgc tcttgaaaag gctcgacagg
       61 tttgtggtga tcagcattgg gatttggatg atcgggaaac taagcctttc ccgttctgtc
      121 caagcttttc gaaagaatat gttcagaaac aggagcgctc caatcagagc cagcaggaac
      181 actgaccaga aggcgagcgg tttcagccag gcaagaagga tccttacgct gtacagggaa
      241 gcgtttccta cggccaaccc aacaatggcc tcgcggtcgg tcactattgc ccagctggga
      301 atgaacggca tgatgagcgt gtcccagcga ttgttcgggt tggcgttgta cttagggaat
      361 gtaagcatgg ggaggaatat ctggatcagg tcatggctgc aaagtgttcc cgcgacagag
      421 agcattacat agatcgtcat catctcgccg gaagtcaggg cggatgacgg ccatcgcttc
      481 tgaacaaaga gattcagaga ggcgataatc aagagagtga acgtcacatg gaaaaacagg
      541 gatatggctg tcgagtgacc tgagtaccag attaactcgg actggaacac ccagagggag
      601 agcagaggca tcaacccaca ggcaattcca actgcccgca gggtgacgat tctgtccgtc
      661 aagccttgct cgggtgtgga aggcacaggt atttcccgct cggtcatccc gcgcactgca
      721 agtctcctac agtggttatg aagtattgac aaaaatctag cgtagtttac agagactgag
      781 cacagtgtca aggaagtttg gcgattccgg caggaaccac agttccattt gcaggaacca
      841 actctttcct cttgacagta agcgttttct gaacaacatt atcgttggag tttgggactg
      901 gtgccgatct agatgtgggc aggctgttca ggttcaaagg acatttgtca acgcagttgg
      961 tagagatgca aagtgatgcg gcattgatac ggaggacttt catggatcgt tattgcagag
     1021 agaacagagg gtttacgctt atcgagcttc tgatcgttgt ggctatcatt ggcattcttg
     1081 cagccattgc cattccgaat ttcctgttgg cgcaagcgag agcgaaggtg gcttcaactc
     1141 acgctggcat gcagacaatt actgtcggag ttgaggcata cgcggtagat aacaatgtct
     1201 atccaagttc ctggctgaac tacggccagg gggcaggctg gatcaacctt tttacagacc
     1261 gactcaaacc cataacgact cctgtggcgt atatcaaaag ggtacctgct gatccttttg
     1321 tcaacaagac ccaaggacag tttttcagcg gcgtaaagtg gtctgtagat gcctatctcg
     1381 cgattcagta tgctgaggac tactttattc ctgtttctat gcgcaatgcc gtcagtccgc
     1441 caagcagacc gggagctggt tacgtggatg gcaaagggat caatgtttgc tacaagctcg
     1501 agggagtcgg tccggatgcc atctacaact ggatgttgcc tgggcggcca gcaaactacg
     1561 agacgatcat ctacgatccc acaaacggta ctgtttccta cggggatatc cggcgctatg
     1621 gtggcaatga cagaggaaac attcattact gagtgcggtg agttccattg tgtttgctca
     1681 tattcggttc agcatgtcgc attggtgtac ccatagttgg acagattgac tgaatcggca
     1741 aatcagcaat ccaatcattg acttcctgct cccaattcct ataattgttt gagcatgggc
     1801 ggttggtttc tgagttccgt tccggggact gagacactgc agattgaggc gaacaagtga
     1861 ttcttgttta cattacgaat ccgagctgtg agcaggccag aacactcgcg aaacacctca
     1921 ttgaaaaacg acttattgca tgtgccaaca tctactctgg cgtgaccagt ctgtaccgtt
     1981 ggaaaggaac tctggcggag gaatctgagt gtgtgatgat cgggaaaact caggaggcgc
     2041 tctttgactc cgttcgcagg gaagtggagc gagtccatcc ctatgagatc ccttgcatcc
     2101 tcaagatccc tgtttcggcg aatgaggctt ttttgaattg gcttgaaggg gaagtctccc
     2161 aagagacggg actctgaatc gttctggtct tctgttcgga cggattgatg cacgagagag
     2221 taaggtatag agatttgatg ccacaaacga caaatacaga tgagggtaca ctcgttctgt
     2281 gctttcgatg gagggcaatg cgatgaatga ttccttctat aggatgctga tggacaatct
     2341 ctcagagggt atctacttcg tggatacgga gcgaaagata acctactgga atcatggtgc
     2401 ggagcgcctg accgggtttc tctccgatga ggtgaaaggg aaactctgtc gcagcaacat
     2461 cctcatgcat gtggacaaca atggccttag cctctgtagg tcgtgctgtc ctgctgccct
     2521 gacactcaat gacgggcagc cccgtgagat tgaggcttat cttcatcaca aagaggggca
     2581 tcgcgttcct gttctgatgc ggctctctcc aattcgcaat gatgtggggg agatcactgg
     2641 agtcatggaa gttttcagtg acaacacagt aaatctttca gcgcgtgatc agattgagaa
     2701 acttcaagtg atggcactgc tcgatccgct gacagaagtg gggaaccgtc gcttcgctga
     2761 agtctgtatt tccgcccgac tcaacgaact caatcgctat gggtggaagt ttggcctgct
     2821 cttcattgac gtggacggtt tcagaagcac gtgtgagaag tatggccatg acatctccaa
     2881 ccgactcctt cgaatgatcg ctcgcacgat tcggaaccgt cttcggtgct ttgatgacgt
     2941 tgctcgatgg ggtgaggatg agtttgttgc cgcggtttcc aatgttgatt cagagagtct
     3001 ctccattgtc gcgaacacac tcagaaggct tatcgaggaa tcaggcttga tcgtcgaaac
     3061 gggtttgatt cagctcactg cctccatcgg ggcaactttg gcagagtccg ttgatagtgt
     3121 ggggcttctg gtagaacgat ctgaacagct gatcaagcaa gccaaaactg atggcaggaa
     3181 caggattgcc acacgactca accggtagaa cccgcctttt tgatctcaca gcgatcgcag
     3241 gcacaagaag aaagcgaccc gtgcggtgag gcacgggtcg cagggtaaca tttggttacc
     3301 atcgagaaca ggcgttctgc tgactcagtt catgtttgtc agcaagagca gaacatcctt
     3361 gttgtcaatg atcccgtcgg cgtcaatgtc ggcgatgccg tagaagttct caccgccgga
     3421 agtagcactc cagatagcgc catacatcat cacgtctccg ccatccaatt gccagttatt
     3481 gttcaagtcg ccgaggattg gcgtatcgcc gttgatcgtg atttcagcag tggccgactg
     3541 tccatcactg tcttccgcga caaaccacac cttgatatca ccttcaggaa tgtccagaag
     3601 gaaggcatcg cggccagtgg caaattccac gagtcctgcc gcctccggct tccaaagcca
     3661 agacaacttt tcgtcaggca ctgcgccctg gtcttccttg tcaaaggcat agcctgagaa
     3721 cacgaccgtg ttgccaattg gatgcttgga gccttcgaag ggtgatgaga tcgtgacgat
     3781 gggtggcttt gattcaacgg tgaaggttcc atcgctgatg tcttcgacgg tacgcactcc
     3841 gtcagaagcg agcacacgaa tcagcatgtc accagaacca gccattcgtt cagcctctga
     3901 gtcaagcgcc atctcaaact gtccgttgat tctggagacc ttgtagccct gcagcggagt
     3961 gacaaccgcc ggaagtccgc ccagcattgg ctcccaggtc tgacctgcgt tatggctgta
     4021 gaacaactgg aaggtcagag tcacgtccgg aatgtccgtt gcagtccaac ggatcttgtc
     4081 ggatggagtc aagacctcac caccgttcgg ataggtcaca gtgacaccca tcggcacacc
     4141 cgccgctacg agctcatcga tcagttgttc acccagtttc aactgtattt tgccgacctt
     4201 gttgctgtag ggcagaatga acgcaaaggg tgaacggcct tccgtctgtg caatgtgcgt
     4261 tgcattcgtg ctgtagcagg cttccatact cgtatcgaaa ggatgagagt agagcagttc
     4321 atccttgtcg gtgcgaagct gaatgaagaa aggacctgct tcattgcttg catccaaggg
     4381 ttcgccagac tcgatcacgt aggagggatc taagtgtccc gaaccgtctc gacgtccaac
     4441 gccggcaatg acatagacct gtctctttcc ttcctcgagt agaggaggtg ctggcgaact
     4501 ggagcgtcgg tcgaagagtc tctcccatgt atagtcggaa acccagcgag gagcgccata
     4561 gttcatgcag gcaatggtgt tggttggcca cgtccgatat gcgtagacat caacgcccac
     4621 ctcccgaatg ctattggggg ccgccgcctc gtcccaagtg tgtggtctgc cgtggttgtg
     4681 accgatttca tgtgccatgg tcatcccacc tcgcggagca ttcggatcgt ctcggttatc
     4741 ccaaccgatg gctgacctcg gagacggata gtagccgaga cccaagagcc ctcctgtcgg
     4801 ggtgttctta ttgaagatgg catagtggta agcatctgac ggcgctccag agacgcttct
     4861 gaagaatgcc cgttgtgatt caacataggc cagcaaagcg tctt
//
LOCUS       DAOQUC010000094         4898 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_19129_length_4898_cov_3.326864, whole genome shotgun sequence.
ACCESSION   DAOQUC010000094 DAOQUC010000000
VERSION     DAOQUC010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4898)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4898)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4898
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19129_length_4898_cov_3.326864"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1569
                     /locus_tag="PKH07_02585"
     CDS             <1..1569
                     /locus_tag="PKH07_02585"
                     /inference="COORDINATES: protein motif:HMM:NF012622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I domain-containing protein"
                     /protein_id="HNT33865.1"
                     /translation="GSDKFSASQSDPVYVQVLPKQSRISLRVPFEGDIQVGTAVPFVC
                     RVSPAPLLDNVTAEFEAWYGVQNGIPSATESKIFVLAQGQYSGTFTPTVDGLAQLQCR
                     WQGDGELSEGSETPPVVLFVDPQNASGRQKTLASSMDPGVCVIAAGVASDQLPTGVRD
                     YLNNFSYSVLSGRRFSHARLKYLNDVAQQDVTWDGLNDDVVDIVQSSASAVEQALNEV
                     APTLGASAPVNLFLVADRGEGNELLMNDGSRLGAAALNAQITSLFGTTRDIRIIVEAS
                     NSGTFAQSLSAANRTVVCSAKSERASFASGGLLSFSQLFWTNIQYGWHVKKSFEQARD
                     YLRAVFGYYGAPTPSLIASSSYPPEYQFFGVSTDAVDMLPPDIDTIFTPTAVERPATV
                     QISAKVFDDIGVHGVSAAVRKPDGTTVSVPMLLQDGTTYAAQLDETVVSELGIYLVTI
                     VAIDEARNASEPRSSEIVVFNSADLNGDGRIDASDLCLLIAEMENPTGFFDIVYDGKL
                     DSLDLLELSRRWTP"
     gene            1605..2597
                     /locus_tag="PKH07_02590"
     CDS             1605..2597
                     /locus_tag="PKH07_02590"
                     /inference="COORDINATES: protein motif:HMM:NF014610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HNT33866.1"
                     /translation="MPKAIGLVSGGLDSALAGRLMKDLDVKVTLLHLKHVFQHRSRGT
                     SLSQLLAESLQVPLIEQEISEDLLSAVCSPRFGHGKNLNPCIDCHVLMLKKARIEMER
                     IGADFIFTGEVLAQRPMSQHRGSLNQVEKQAGVKGILLRPLSAKLLEPTPMETSGMID
                     REKLLDLSGRSRTRQLQLAQQWGVEHFGTPAGGCLLTDPEFTKRLEKTLERGCPSVAD
                     ADLLRYGRHFLLDDNTKAVVGRDSADNDEIESLACEGDIFLFSESPPGPLVLLRGTRS
                     EGNLRLAALLQKRYGKARDRESLRVSFRPVGSEQVSHIEAQGSLSEESLDQMRI"
     gene            2594..3289
                     /gene="nfi"
                     /locus_tag="PKH07_02595"
     CDS             2594..3289
                     /gene="nfi"
                     /locus_tag="PKH07_02595"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: protein motif:HMM:NF008629.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HNT33867.1"
                     /translation="MIHSHSPYQQAMEMQQSLREKIVLRDTFDLRGIRFVAGADLCFA
                     KEADIGFAVILVLSLPEMKIVEQASAVGRISFPYIPGLLSFRELPLLCEAYDKLQCKP
                     DVLICDGQGLAHPRRFGLACHLGLELDVPTVGAAKSRLVGEYTEPASPRGSHSALIHQ
                     GERVGEVVRTRDRVKPMFISPGHKVGFETAVELVLRLTPRYRVPEPTRQADQRVACLK
                     RDWLAQRAKNAIV"
     gene            3310..4278
                     /locus_tag="PKH07_02600"
     CDS             3310..4278
                     /locus_tag="PKH07_02600"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HNT33868.1"
                     /translation="MPDIISLGEALVDFVSTVSGVSVKDAPAFEKAPGGAPANVAAGL
                     AKLGVSVGFAGKVGQDAFGLCLKETLEHWGVDTHGLILDSACRTTLAFVSLTADGQPS
                     FAFYRNPGADMMLTPDEIDPEWIGTAKILHFGSISLLDAPARDATRTAIAQARRGGAL
                     ISYDPNLRPALWSDRQSAFSFIREGMEQAEIVKMSEEELIEIQCASDTRQGMEQLMKQ
                     GKIAVFVTRGAEGCCVRCAEGFAEQPAMKVDPIDTTGAGDGFVAGVLSRVLQSLKEGS
                     DPHRLPLDWWRETARFANTVAGLSIGSKGAIPALPTLEQVQRALGQ"
     gene            4397..>4898
                     /locus_tag="PKH07_02605"
     CDS             4397..>4898
                     /locus_tag="PKH07_02605"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNT33869.1"
                     /translation="MPLTGMRVLVTGAGGFLGSHLTESLIQKGASVRAFLRYTSQRQV
                     GNLAFLPEEILKELDLYFGDIRDADAVLNAGRNCAAILHLAANIAIPYSYAHPGDTVA
                     SNVIGTFNVLTAAKALNTRRVLVVSSSEVYGTARTERISEQHPLQAQSPYAASKIGAE
                     KLAESFH"
BASE COUNT         1053 a         1264 c         1421 g         1160 t
ORIGIN      
        1 ggctcggaca aattctctgc ttcccagagt gatcctgtct atgttcaggt tctcccaaaa
       61 cagtcaagaa tctcattgcg cgttccgttt gaaggagaca ttcaggtggg tactgcggtg
      121 cccttcgtct gccgagtcag tcctgcgccg ctgttggaca atgtcacagc ggagttcgag
      181 gcatggtatg gagtgcagaa cggtattcct tctgcaaccg aatcgaagat ttttgttctg
      241 gcacaggggc aatacagtgg gacattcacc ccgaccgtag atggcctggc gcaactgcag
      301 tgtcgctggc aaggcgatgg agagctatca gaaggttcag agacgccgcc ggtggttttg
      361 tttgttgatc ctcagaatgc atcaggccga cagaagactt tggcgtcgag tatggatccc
      421 ggtgtctgtg tcattgcagc gggagttgct tcggatcaat tgccaaccgg tgttcgcgat
      481 tatctgaaca acttctctta ctcggtgctc tcgggcaggc ggttcagtca cgctcgcctg
      541 aaatacctca acgacgtggc gcaacaggat gtcacttggg atgggctcaa tgatgatgtg
      601 gttgacatcg ttcaatcttc ggcatctgct gtcgaacagg cattgaatga ggttgcacca
      661 acgcttggag caagtgcccc cgtgaacttg ttcctggtcg ctgatcgagg agaaggcaac
      721 gagttgctga tgaatgacgg aagccgtctg ggcgctgctg ctctcaatgc acagatcacc
      781 agtcttttcg gaacaacccg ggatattcgt atcattgttg aagcgtctaa ctcgggcacc
      841 tttgcccagt cgttgagcgc tgccaatcga accgtcgtct gctcagcaaa atccgaacgc
      901 gcttcctttg cttcaggtgg cctgctctcg ttctcccaac tcttctggac aaatatccaa
      961 tatggttggc atgtcaagaa gagctttgaa caggcgcggg actatctgcg cgctgttttt
     1021 ggatattacg gcgcaccgac gccatcgctc attgcctcca gttcctaccc accggagtat
     1081 cagttcttcg gtgtttccac cgatgccgta gacatgctgc cacccgatat tgacaccatc
     1141 ttcactccca ctgcggtaga gcgtccggcg acggtgcaga tttccgccaa ggtgttcgat
     1201 gacatcggag tccacggagt cagcgctgct gttcggaagc ctgacgggac gactgtttcg
     1261 gttccgatgt tgctacagga cggaacaacc tatgctgccc aactggatga gacggtggtc
     1321 tcagagttgg ggatctacct ggtcacgatt gttgccatag atgaagccag aaacgcatca
     1381 gagcctcgat cgtcggagat cgttgtgttc aactcggcgg atctcaatgg ggatggccgt
     1441 attgatgcca gcgatctctg tcttctcatt gcggagatgg agaacccgac tggattcttt
     1501 gatatcgttt atgacgggaa gcttgactct ctcgatcttc tggaactgtc ccgaagatgg
     1561 acgccctgac caagcgattc agattactca ggagcctcac gcgcatgccc aaagcgattg
     1621 gattggtttc cggagggctg gacagcgccc ttgccgggcg actcatgaag gatctggacg
     1681 tgaaggtcac gctccttcat ctaaagcacg ttttccagca ccgttccagg ggaacctccc
     1741 tctcccaact gcttgcagaa agccttcagg ttcctctcat tgaacaggaa atatccgaag
     1801 acctcttgag cgctgtctgt tcccctcgtt ttggacatgg gaagaatctc aacccctgca
     1861 ttgattgcca tgtgctgatg ctcaagaagg cgcgtatcga gatggagcga atcggcgccg
     1921 acttcatctt caccggagag gtgctcgcgc agcgtcccat gtcgcagcat cggggctctc
     1981 tgaatcaggt ggagaaacaa gcgggagtga agggaattct tcttcggcct ttgagtgcca
     2041 aactacttga gcccacgccc atggaaacat cagggatgat tgatcgggag aaactgctcg
     2101 atctttccgg ccgttctcgc accaggcaac tgcaactggc tcagcaatgg ggcgttgagc
     2161 attttgggac accggcaggc ggttgcctgt tgacggatcc agaattcacc aagcgccttg
     2221 aaaagacgct cgagcgaggc tgcccttctg tggcggatgc cgatcttctt cgttacggga
     2281 ggcattttct tctcgatgac aacaccaagg cagttgtggg gagggactct gcagacaacg
     2341 atgagataga gagcctggca tgcgagggag acatctttct gtttagcgaa tcgccaccgg
     2401 gtcccctggt gcttctgcgc ggcacgaggt ctgaggggaa tctgcggctg gctgcgttgc
     2461 tgcaaaaacg ctacggcaag gcgcgagaca gagaatcatt gcgggtttcc tttcgaccgg
     2521 tcggaagtga acaggtctca cacatcgaag cccagggaag cctctccgag gaatctctcg
     2581 accagatgcg gatatgattc attcccattc gccgtatcag caagccatgg aaatgcagca
     2641 gagtctgcga gagaagatcg tactccgcga cacatttgat ctgcggggaa tacggtttgt
     2701 tgctggtgcg gacctgtgtt tcgcgaagga agcagatatc ggctttgcgg tgatcctggt
     2761 tttgtctctt ccagagatga agattgtcga gcaggccagt gcggtgggaa ggatttcatt
     2821 cccttacatt ccagggcttc tttcctttcg ggagcttcct ttgttgtgtg aagcgtacga
     2881 caagttgcag tgcaaaccgg atgtactgat ctgcgatggt caaggtctcg cacatccgag
     2941 gcgctttggc ctggcgtgcc acctcggact tgaactggac gtgccaaccg tcggcgctgc
     3001 caagagcagg ctcgtcgggg agtatacgga acccgcctca ccgcgaggct cacacagcgc
     3061 tctgattcat cagggagaac gggttggcga agttgttcgg acgcgggatc gggtcaagcc
     3121 tatgttcatc tcgccaggtc acaaagtagg atttgaaacc gccgtggaac ttgtgctgcg
     3181 cctcacgccc cgataccgag ttcccgaacc tacgcggcag gcggatcagc gagtcgcttg
     3241 cctgaaacgt gattggcttg ctcaaagggc aaaaaatgct atcgtatgag aagaagaaag
     3301 gagcgctata tgccagacat tatcagctta ggtgaagcgc ttgttgattt tgtttccacc
     3361 gtatccggtg tttctgtcaa ggatgcgcca gcatttgaga aagcgccggg aggtgcgccc
     3421 gcaaatgttg ctgcagggtt ggcgaaattg ggggtttccg tcggctttgc cggcaaagtg
     3481 ggtcaggatg cctttggtct ttgtttgaaa gagactctgg agcattgggg cgtagatacg
     3541 catggactga tactcgattc cgcatgtcga acaacacttg cctttgtgtc tctgacagct
     3601 gatggtcagc cgagtttcgc attctatcga aatcccggcg cggacatgat gctgacacca
     3661 gatgagattg atcccgaatg gattggcact gcgaagattc tccatttcgg ttcgatttct
     3721 ctcctggatg ctcctgcccg tgacgcgaca cgaacggcaa tagcacaagc ccgacgtggc
     3781 ggtgcgctga tctcctatga tccgaatttg agacccgcac tctggagcga tcggcagtct
     3841 gctttctctt tcatccgtga ggggatggag caggcggaaa ttgtcaagat gagcgaagaa
     3901 gagctcatcg agattcagtg tgcgtccgac actcgccaag gcatggaaca gctcatgaaa
     3961 cagggcaaga tagcggtctt tgtgacgaga ggggctgaag gctgctgtgt tcgatgtgct
     4021 gagggctttg cagagcaacc ggcgatgaag gttgatccga ttgacaccac tggtgcggga
     4081 gatggtttcg tcgcgggtgt tctgtcaaga gttttgcagt cattgaagga aggttcggat
     4141 ccacaccggt tgcccttgga ctggtggcgt gagacagccc gatttgccaa tacggtggcg
     4201 ggcttgagca tcggaagcaa gggagctatt cccgccctgc cgacacttga gcaagtccaa
     4261 cgggccttgg gtcaataacg gagaagcagg atctgtccgt tgtttttgtg gtgcaggcat
     4321 cttgcctgca tggagtcagc gcaggctgga agcccgcacc acaatcgaga cggacaggat
     4381 aacggagaat cagcaaatgc cactgacagg gatgcgtgtt ctggtcaccg gagccggtgg
     4441 tttcctggga agccatctga cagagagcct catccagaag ggagcctcag ttcgtgcgtt
     4501 tcttcggtac acgtcgcagc ggcaggtcgg caatctcgcc tttcttcccg aagagattct
     4561 gaaggaattg gatctctatt ttggggacat ccgtgatgcg gatgccgttc tgaacgcagg
     4621 ccgcaactgt gctgcgattc tgcaccttgc cgccaacatc gccatcccct attcctacgc
     4681 tcatccaggt gacaccgtgg caagcaatgt cataggcaca tttaatgtgc tcactgcggc
     4741 caaggcactc aatacgcgga gagtgcttgt ggtgtcttca tcggaagtct acggaactgc
     4801 acgaaccgaa cgcatcagtg agcaacaccc tcttcaggcg cagtcgccct atgctgcgtc
     4861 gaaaattgga gccgaaaaac tggctgagtc ttttcatc
//
LOCUS       DAOQUC010000095         4888 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_19195_length_4888_cov_3.710118, whole genome shotgun sequence.
ACCESSION   DAOQUC010000095 DAOQUC010000000
VERSION     DAOQUC010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4888
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19195_length_4888_cov_3.710118"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..992
                     /locus_tag="PKH07_02610"
     CDS             <1..992
                     /locus_tag="PKH07_02610"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HNT33870.1"
                     /translation="ESLLAEFPESQHSAEALTALALIAQASGDHQLSQQRWARLTKEY
                     PNSEGAAYSELSRADELAQTGKVEEARTLYYQIIQNSPPGSKFAGEAMDKLSVINTSY
                     LFSAVPNEWNQLVQIQKGDSLMKLAYKYKTTANFIAKANGRDLNWSLVPNQRITVPTI
                     ESYRIVVDKDDLHLYLYTGSGKFIKRYLVGIGRMDHLTPPGQYVIWHKLKSPVWHDRD
                     TGRIVKPDSPDYPLGTRWMTISPASNPEAFSRLGIHGTNEPETIGQRASDGCVRMSNE
                     ESEELFIIVPKGTPVEIVQKRPAPQDSGSQPQQSQPSPLQDWLEPRIQGEATG"
     gene            1149..2165
                     /gene="aroF"
                     /locus_tag="PKH07_02615"
     CDS             1149..2165
                     /gene="aroF"
                     /locus_tag="PKH07_02615"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HNT33871.1"
                     /translation="MIIVMEAGAHKENVDRVIAEVESLGYRAHPLFGTDRTVIACLGD
                     ERGKERIQTLDSLPEVERVVPILRPFKLSSREVRPETTIVDVKGVKIGGKALVVMAGP
                     CAVESREQILEVAHAVKEAGAHILRGGAFKPRTSPYSFQGLAEQGLKFLAEASQATGL
                     PVVTELVDAHQLDMVQDYVDMIQIGARNSQNFFLLSSLGRAVKPVLLKRGMMSTIEEY
                     LMAAEYVVTNGNGNVVLCERGIRTFETYTRNTLDLNAVAAMKKLSHLPVTVDPSHGTG
                     RWDLVPPMCYASVAAGADGLLVEVHTHPDKAVSDGPQSLKPEKFQAMVKTLRAFAEAA
                     GRTL"
     gene            2175..2744
                     /gene="rsmD"
                     /locus_tag="PKH07_02620"
     CDS             2175..2744
                     /gene="rsmD"
                     /locus_tag="PKH07_02620"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HNT33872.1"
                     /translation="MSLRIIAGQFKGRKLFSPKGEEVRPTAAVVREALFSILGYEIEG
                     ARVLDLFAGTGSIGFEALSRGAQRVTFVERLPDARNAILKTAEHLGVTNQIEVIDANA
                     AGKIRHRIDQEVQFDLVFIDPPYGHERPAKILGRIISEGLLAEGAFVVYEQQRNEVQA
                     RPVEGLVHVDERRYGRTVLTFFTRSENDG"
     gene            2848..3327
                     /gene="coaD"
                     /locus_tag="PKH07_02625"
     CDS             2848..3327
                     /gene="coaD"
                     /locus_tag="PKH07_02625"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HNT33873.1"
                     /translation="MTKAIYPGSFDPVTLGHLDLIRRSSTLFDSLVVAVTNNRVKQPL
                     FTLEERVAMLQEVTADIEGVQVDSFSGLLVDYARSKGAQIVLRGLRAISDFEYEFQMA
                     LTNRKIDPVVNTVFMMPSEEFSYLSSSIIREIASLGGDVTPFVPAPVVEKLRQKFPG"
     gene            3375..4379
                     /gene="pta"
                     /locus_tag="PKH07_02630"
     CDS             3375..4379
                     /gene="pta"
                     /locus_tag="PKH07_02630"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041734229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HNT33874.1"
                     /translation="MDPLVRIVSEARKNKKTVILPEAEDARMLVAAAKATKEGIADIV
                     LLGDPDKVSAQAKAEGVDLKGVRITDHLKSAELSTYANAYYELRKHKGVTQDQAATLM
                     KDPLFYGAMMVRNNALDGMVAGAVNTTANVIRASVQIVGMPKGMKTVSSCFLMVLPTP
                     EFGVDGSLIYSDCGVVPDPTAEQLVDIAAAAAQSCRALIGAEPVIAMLAFSTYGSASH
                     PIVDKVIQATKLAKEKYPNLQIDGELQADSALIPSIGSKKAPGSPVAGKANTLIFPDL
                     NAGNIAYKLTERLAKATAIGPLLQGVAKPVNDLSRGCSAEDIYNTIAITVVESQMVEK
                     "
     gene            4420..4599
                     /gene="rpmF"
                     /locus_tag="PKH07_02635"
     CDS             4420..4599
                     /gene="rpmF"
                     /locus_tag="PKH07_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HNT33875.1"
                     /translation="MANPKHRHSRTRGRKRRTHDSLTSPQHNECPNCHEPKLPHVVCP
                     HCGHYKGVKVIGKEE"
     gene            4596..>4888
                     /locus_tag="PKH07_02640"
     CDS             4596..>4888
                     /locus_tag="PKH07_02640"
                     /inference="COORDINATES: protein motif:HMM:NF014554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HNT33876.1"
                     /translation="MNETCIAVDALGGEYSPGSVVEGIRLLDGSLPVKFLLVGDQERI
                     RACNLDGLNVEIVHTDQVVDMAESPGQALKRKRQSSIAVAVGLVKEKRAQAVFS"
BASE COUNT         1194 a         1253 c         1366 g         1075 t
ORIGIN      
        1 gggagtctct tctggccgag tttccagaaa gccagcattc cgcggaagcc ctgaccgcgc
       61 ttgccctaat cgctcaggcc agtggagacc atcagctgag tcagcaacga tgggcgcgtc
      121 tcaccaagga gtatcccaac agtgaagggg cggcatattc cgaattgtcc cgggcagatg
      181 aactggcaca aacaggcaaa gttgaagaag ccagaaccct ctactatcag atcatccaga
      241 acagcccgcc cggcagcaaa tttgccggcg aggcgatgga taaactcagc gtgatcaaca
      301 catcgtactt gttctcagca gtccccaacg aatggaacca gttggttcaa attcagaagg
      361 gcgattcgtt gatgaaactg gcgtacaagt acaagaccac ggctaacttc atcgcgaaag
      421 caaatggacg cgatctgaac tggtctttgg tgcccaacca gcggatcacg gtgccaacca
      481 tagaatccta taggattgtt gtggataaag atgacttgca cctttatctc tacacaggct
      541 ccggcaagtt catcaagaga tatctggtcg gaatcggacg catggatcat ctcaccccac
      601 caggacagta cgttatttgg cacaaactaa agtcacccgt ctggcatgat cgggataccg
      661 gacgcatcgt gaaaccagac agtcccgact atcctctggg aacacgctgg atgaccattt
      721 cgccggcatc gaatccagag gccttttcac gcttgggcat acatggtacc aacgagcctg
      781 agaccatcgg ccagcgagcc agcgacggtt gtgtccggat gagcaatgag gaatccgaag
      841 agttgttcat catcgtgccc aagggaactc cagtggagat cgttcagaag cgtccagcac
      901 ctcaggattc cggttcccaa ccccagcaga gccagccatc tccactccag gactggctcg
      961 aacccagaat ccagggagaa gcgaccggat aatttgtttc tctcctccat cccaaggaaa
     1021 aaccaataac tcattgacat ggataaactt atcgggtact acatccactc tggtaacctg
     1081 cgatctattg ttgagatgcg cagaattgaa aaccgtgcgt ggtttcccat tagaaggagt
     1141 ttacaacaat gatcatcgtc atggaggcgg gtgcccacaa ggaaaatgtg gatcgggtga
     1201 ttgccgaagt cgagtcgctt ggctatcgag ctcatccact gttcggaacg gacagaacgg
     1261 tcattgcctg cctgggtgat gagcgtggca aggaacgcat tcagacactg gacagtctgc
     1321 cagaagtcga gcgtgtcgtc ccgattctac gacccttcaa actgtccagt cgggaagtcc
     1381 gacccgagac aaccatcgtg gatgtcaaag gggtgaaaat cgggggcaaa gccctggttg
     1441 tcatggcggg gccgtgcgcc gtagagtcca gagaacagat attggaagtg gcgcacgcag
     1501 tcaaagaggc gggtgctcat attctacggg gtggagcctt caaaccaagg acttcgccgt
     1561 actcctttca aggtcttgca gaacagggtc tgaaattcct tgcggaagca agtcaggcga
     1621 ctggacttcc cgtcgtcacg gagctggtgg atgctcacca actcgatatg gttcaggact
     1681 acgtggatat gattcagatc ggcgcacgta attctcagaa cttctttctt ttgagtagtt
     1741 tgggacgagc ggtcaaaccg gttctgctca aacgaggcat gatgagcacc attgaggagt
     1801 acctcatggc tgctgagtat gtggtgacca atggaaacgg caatgtagtg ttgtgcgaac
     1861 gagggattcg aacgttcgaa acctatactc gaaacacact tgacctgaat gcggtggcag
     1921 ccatgaagaa actctctcat ctccctgtca cggtggatcc aagccacggg acaggtcgtt
     1981 gggatcttgt gccgcctatg tgttacgctt cggtggcggc tggggcggac ggtcttttgg
     2041 tggaagttca tacacatccc gataaggcag tctccgacgg gcctcagtca ctcaaacctg
     2101 agaagtttca agcaatggtc aagacgctgc gagcctttgc ggaagccgca gggagaacac
     2161 tgtaggacgc aacattgagc cttcggatta ttgcgggaca attcaaagga cggaaactct
     2221 tctctccaaa aggtgaggag gttcgcccta ctgccgctgt cgttcgagag gcgctcttca
     2281 gcatcctggg ctacgagatc gaaggggctc gtgtcttgga tctatttgcg ggaactggca
     2341 gtatcggttt tgaggccctc agccgcggtg cccagcgagt cacctttgtc gaaaggctgc
     2401 cagatgcccg aaacgcaatt ctgaagacag cggagcatct cggcgtcacc aaccagatag
     2461 aggtcattga cgccaatgcg gcagggaaaa tcagacatag gattgaccaa gaggtgcaat
     2521 ttgatctagt attcattgat ccgccttatg gtcatgaaag accggcgaag atattggggc
     2581 gtatcatttc ggagggtctg ctcgccgagg gtgcatttgt tgtctacgag cagcagagaa
     2641 atgaagttca ggccagaccg gtagaaggtt tggtgcacgt tgatgagcgc cgttacggca
     2701 gaacggttct gacgttcttt acccgttctg aaaatgacgg ttagtatcca gcgcacgggg
     2761 tgctatgccc ttgtgttggg tgtttgttta ggccggaaac ccgactgctt gacggtctgg
     2821 tatccagaag aactgggagg cacgggattg accaaagcga tatatccggg gagttttgac
     2881 cctgtaacgc tcggtcattt ggacttgatc cgacgcagca gcaccctttt tgacagcctc
     2941 gttgtcgctg tcaccaacaa tcgggtgaag cagcccctgt tcacccttga ggaacgagtg
     3001 gcaatgctcc aggaagtgac agcggacatc gagggagttc aggtggattc tttctccgga
     3061 ctgcttgtgg actatgcacg atccaaagga gcacagatcg ttcttcgagg tcttcgcgcc
     3121 atctctgatt tcgagtacga atttcagatg gctcttacca accgcaagat cgatcctgtt
     3181 gtcaataccg tattcatgat gccgagtgag gagttttcct atctcagttc cagtatcatt
     3241 cgtgagattg cctcactggg tggggatgtg acaccttttg ttccagcacc tgtggtggag
     3301 aaactgaggc agaaattccc tgggtgaggt tctcaccttg ggattcaaaa atcaaagtct
     3361 aaggagattg agccgtggat ccgcttgtaa gaatcgtcag tgaagccaga aaaaacaaga
     3421 aaaccgtcat tctgccggaa gccgaggatg ctcggatgtt ggtcgccgct gccaaggcaa
     3481 ctaaggaagg gattgccgat attgttcttc tgggcgatcc ggacaaggtc tccgcacaag
     3541 cgaaggctga aggtgtggat ctgaagggcg ttcgtattac agatcatctc aagagcgccg
     3601 agttgagcac ctacgccaac gcctactacg aacttcgaaa gcacaagggt gtaacccagg
     3661 atcaggccgc aacgctcatg aaggatccgc tgttctacgg tgccatgatg gttcgcaaca
     3721 atgccttgga tggcatggtg gcaggtgcag tcaacaccac agcgaatgtc attcgcgcca
     3781 gcgtccagat cgtcggcatg cccaaaggga tgaagaccgt ttcgtcctgc ttcctgatgg
     3841 ttcttccgac acctgagttc ggtgttgacg gctccctgat ttactcagat tgcggtgtcg
     3901 ttcccgaccc cacggcagaa cagctggtgg acatcgccgc cgcggcggct cagtcgtgcc
     3961 gcgccctgat cggcgctgag cctgttatcg cgatgttggc cttctccaca tacggcagtg
     4021 caagccatcc cattgtggac aaagtgatcc aggcgacaaa actcgcaaag gaaaagtacc
     4081 ccaaccttca gattgatgga gagttgcagg ccgactcggc gctcattcct tctattggca
     4141 gcaaaaaggc tccgggaagt cccgttgctg gcaaagcgaa taccctgatc ttccctgatc
     4201 tgaatgccgg taacatcgcg tacaaactca cggagcgttt ggcaaaagca accgccatcg
     4261 gacctctgct tcagggcgtt gccaagcccg tcaacgactt gtcccgaggc tgctccgccg
     4321 aggatattta caatacaatt gccatcacgg ttgttgaatc gcagatggtg gagaaataag
     4381 acaacgtctt gtttgaaatg agaccggagg tgaattgaat tggcgaatcc gaaacacaga
     4441 cattcccgaa ctcgtggccg caagagacgc acacacgact cgctgacgtc tccgcagcac
     4501 aacgagtgcc caaactgtca cgaaccgaaa ttgccgcacg tggtctgccc acattgcggt
     4561 cattacaagg gcgttaaggt aatcgggaaa gaagaatgaa cgaaacatgc attgccgttg
     4621 atgccttggg cggagagtac tcccccggct cggtggttga agggattcgg ctcctcgatg
     4681 ggtcgctgcc cgtcaagttt ctgctggtcg gcgaccagga gcggattcgc gcctgcaatc
     4741 ttgacggctt gaacgtcgag attgtccata ccgatcaggt tgtggacatg gctgagtcac
     4801 cgggacaggc gctcaaacgc aagagacagt cttccatcgc cgtagcggtt ggtttggtta
     4861 aggagaaaag ggctcaagcg gttttctc
//
LOCUS       DAOQUC010000096         4881 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_19237_length_4881_cov_3.018027, whole genome shotgun sequence.
ACCESSION   DAOQUC010000096 DAOQUC010000000
VERSION     DAOQUC010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4881)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4881)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4881
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19237_length_4881_cov_3.018027"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..735)
                     /locus_tag="PKH07_02645"
     CDS             complement(<1..735)
                     /locus_tag="PKH07_02645"
                     /inference="COORDINATES: protein motif:HMM:TIGR03663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03663 family protein"
                     /protein_id="HNT33877.1"
                     /translation="MKENKLNITVATLLFLAILGIGAFFRLSHLSLMAYHHDESIHEY
                     YSYQLYRYGPGAPQLGKSSPPYYSAVYHGPFLYHFSALFFLLFGVNDFTGRLPFAVYG
                     IAMLWIAYSLKLVTGHRKALLIMLFCAVSPVLTYFSRFARNDVLLGTDNLAMLAFTLL
                     YFHTRKARYFLLAALAAVVGYCTKENSYVGGAVLCSFLVLYCVARLVPAIRPQLLRKI
                     LMIAAPIVAMAALFVAPGKTLKTAAVP"
     gene            complement(741..3659)
                     /locus_tag="PKH07_02650"
     CDS             complement(741..3659)
                     /locus_tag="PKH07_02650"
                     /inference="COORDINATES: protein motif:HMM:TIGR03663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03663 family protein"
                     /protein_id="HNT33878.1"
                     /translation="MSVLEKNVKLTNVIVILGILIALGGFFRLYDLGRMAYHHDESMH
                     AYYSFRLFKYGPHSSQIAGDPSRYTPVYHGPFQYHIGALFFFLFGDSDYTGRLPHAVF
                     GIVLLCLAYGLSQFFGRRTALITVFLLAVSPVLVYFSRFTREDTYFAVSNFALVLFSL
                     LYLKTRRPLYFHLSALGLILGYCTKENSYMSGAILGSFLVLYVLWGLLSENRNRHLGL
                     VFSTYHQYTKAWTLYGLFSVFMFGYAYAVVHQIKLFGKVLATRASTAHDNFETSITGL
                     GVLYWIIALIVSAAVIWLVAWARRSFRRYDPYLSDKEKRWEEPDDAVEWLKRLLTDNQ
                     ELLLSICLVVCVYVVLFTRMFSNSGGIWSGVYDYLAYWMAQQEEPRIPGPKSYHIVRL
                     LVYELLAVVFSVIGLFYVVYRSTLHLFAKGTADSQAEVPQPPSAIGVFLLYWSLMSVF
                     IYSHLNEKCPWLLTHQALPMVLFAGWVFGELWERLRGHWGWSVLRGALLVVFVILAAA
                     MVRSTVVLNLFQNDDPKEMIVYVQSQQDVIGIRDEIEDLAFRTVKRKNLKVLLTGKAP
                     WPFSWYMRNYPYTSFGTVTEGTDADVVICDDIQKKDAKAALGNDFDEGRTYHLQGWWV
                     VEHEDLPKGFKQRKDALLKYFIYREPWGKKTFGHWDIALFVRSDIYGVGGRTGVSIPA
                     GYKNSPAYPSVLKEWGKAGAAPGQFNKPKGIAIAPNDAGLYVADSGNHRIQKFDLDGN
                     SLLLWGDQGTAPAQFNEPSGIAVGPDGMVYVADLWNARIQKFDSEGKFITQWDAGDGR
                     FYGPRDVGVSPKNEVFVADTGNKRIMVYSSDGQFLRKWGSGGKQQGQFDEPVGLAISS
                     GNEVFVADTVNQRVQVFDDMGTFKRQWAVLGWADRYTQPYLALDSRGTVFASDAVQNV
                     IHRFLPDGSAVTRFGSKGTAPGQMDRAVGIAVDSQDNVYIADTDNNRIQKWAPLRHIN
                     "
     gene            3940..4728
                     /locus_tag="PKH07_02655"
     CDS             3940..4728
                     /locus_tag="PKH07_02655"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HNT33879.1"
                     /translation="MSCPRFDFHMHPKHAGCANATMELPAIVQQSHSVGVVAIGITDH
                     LNRPEQIPLHRQIRDELLTMQSPVDVFFGVELNFTGCDQGFVFSEEIKQEYQFQFAIG
                     GIHSTYLNEFDLDKIIEIQHRHHLKTCEDSLVDVLVHPYWFSRKEFKDKNWPFIESMK
                     PVRESLTRELAQAAKETGTAIEINVGANIRRQKDYVEEYVDYLSIFAQEGTMFTIGSD
                     AHSIDHLLDIKLAWDVVDRLGIPEERIWRPATEPLLKACEVAKS"
BASE COUNT         1263 a         1274 c         1270 g         1074 t
ORIGIN      
        1 aggaacagca gccgttttca aagtcttgcc tggggctaca aacagcgctg ccatggcaac
       61 aataggtgct gcgatcatca gaatctttct cagcaactga ggtctaattg ccggtaccag
      121 tctggcgaca caatagagca ccaggaaaga gcacagcacc gcgccgccta catacgaatt
      181 ctcttttgtg caataaccga caaccgcagc cagagccgcc agcaggaaat atcgagcctt
      241 ccgggtgtga aagtacaaca gtgtgaatgc aagcatcgcc agattgtctg tgcccagaag
      301 tacatcattt cgggcaaacc gagagaaata agtcagaacg ggcgacaccg cgcagaagag
      361 cataatcaga agtgccttgc gatgtcccgt gaccaacttc aggctgtatg ctatccacaa
      421 cattgcaatg ccgtaaacgg cgaaaggaag ccgtcctgta aaatcgttca ctccgaacag
      481 caggaagaag agggcactga aatggtagag aaaaggtcca tggtacacag cgctgtaata
      541 cggtggagaa gacttgccca actgaggagc acctggcccg tatctgtaaa gctgatagga
      601 atagtactca tggattgact cgtcgtggtg ataggccatc agggagaggt ggctcaaacg
      661 gaagaaagcc ccgatgccca gaatggcgag gaagagaagc gtggcaaccg tgatattgag
      721 tttgttttct ttcattgcac tcagttgata tggcgcaggg gcgcccattt ctggattctg
      781 ttattgtctg tgtcggcgat atagacgttg tcctgtgagt caaccgcaat gccgaccgcc
      841 cgatccatct gtccaggtgc cgttcccttg cttccgaaac gggtgaccgc cgagccatcc
      901 ggcaggaacc ggtggatgac gttctgaaca gcatctgatg caaaaaccgt tccgcggcta
      961 tcgagcgcaa ggtagggctg cgtgtagcgg tcggcccaac ccaacactgc ccattgcctc
     1021 ttgaacgtgc ccatgtcgtc aaagacctga acgcgctgat ttactgtgtc cgcaacgaaa
     1081 acttcattgc ccgaactgat cgccagaccg acaggctcgt caaactgccc ctgttgtttg
     1141 cctccggaac cccatttgcg cagaaattgt ccatcgctgc tgtagaccat gatgcgtttg
     1201 tttcctgtat cggcaacaaa gacctcattc ttcgggctga caccgacatc cctcgggcca
     1261 tagaagcgcc cgtcaccagc atcccactga gtaatgaact tcccttcgga gtcaaacttc
     1321 tgaattcggg cgttccacaa gtcagccacg tagaccatgc catccggccc cacggcaatg
     1381 ccacttggtt cattgaactg cgcgggggcg gttccttgat ctccccacag aagcaacgag
     1441 tttccgtcca gatcaaactt ctgaatcctg tggttcccgg aatcggccac atagagccca
     1501 gcgtcattgg gtgcgatcgc gattcccttg ggcttgttga actgccccgg agcggcacct
     1561 gccttgcccc attccttgag cacggaagga tatgccggtg aattcttgta ccctgcaggg
     1621 atcgaaaccc ctgtacggcc accaacgcca tagatgtcac ttcggacaaa cagggcaatg
     1681 tcccaatggc caaatgtttt ctttccccaa ggctctctgt agatgaaata cttcagcagg
     1741 gcatccttcc tctgcttgaa ccctttgggg aggtcctcgt gctccaccac ccaccacccc
     1801 tgaagatgat aggtgcgacc ttcgtcaaag tcatttccca acgctgcctt ggcatccttt
     1861 ttctggatgt cgtcgcagat gacaacgtcg gcgtcagtgc cttctgtcac cgttccaaaa
     1921 ctggtgtatg gatagttcct catgtaccag gaaaatggcc atggagcctt gccggtcaga
     1981 agcactttga gattctttct tttcaccgtt cggaatgcca gatcctcgat ctcatctcgg
     2041 ataccgatga cgtcctgctg cgactgcacg tagacgatca tctccttggg gtcatcgttc
     2101 tggaacaagt tcaacacaac ggttgagcga accatggcgg ctgccagaat cacaaagaca
     2161 accaacaatg cgcctctgag aacagaccat ccccaatgtc ctcgcaagcg ttcccagagt
     2221 tctccgaaca cccacccagc gaacagcacc attgggagag cctgatgtgt cagaagccag
     2281 ggacatttct cgttcaaatg cgaatagatg aagacagaca tcaacgacca gtagagcagg
     2341 aagactccga tggcagatgg gggttgagga acttcagcct gtgaatcagc ggtgcccttg
     2401 gcgaagagat gcagggttga tcggtacaca acgtagaaca agccaatcac cgaaaacacg
     2461 acggcgagca gttcatagac gagaagacgt acgatatgat agctctttgg gcctggaata
     2521 cgtggttctt cctgctgagc catccagtaa gccagatagt cgtaaactcc actccagatg
     2581 ccaccactgt tgctgaacat tcgggtgaag agaacaacgt agacgcacac aaccaagcaa
     2641 atggagagaa gcaactcctg gttgtcggta aggagacgct tgagccattc gaccgcgtca
     2701 tcgggctctt cccatcgttt ctccttgtct gagagatagg gatcgtatcg ccgaaaagat
     2761 cttcttgccc aagccacaag ccagatcact gctgcggaca ctatcagggc gattatccag
     2821 tagagaaccc caagacctgt aattgaagtc tcgaagttgt cgtgcgccgt tgaagccctt
     2881 gtggcgagaa ccttgccgaa caacttgatc tgatgaacca cagcataggc gtatccgaac
     2941 atgaagacac tgaaaaggcc gtagagcgtc caggctttcg tgtactgatg ataggtcgag
     3001 aagaccagac caaggtgtct gtttcgattc tcagagagaa gaccccaaag cacatagagc
     3061 acgaggaacg agccgagaat ggcaccggac atgtaggagt tttccttggt gcagtatcca
     3121 agaatcagcc ccaatgcaga cagatggaag tagagtggtc tacgcgtctt taagtagaga
     3181 agtgaaaaca gaaccagagc gaagttgctc accgcgaaat aggtgtcctc acgagtgaag
     3241 cgggagaagt agaccagcac cggtgaaacg gcaagcagaa agaccgtgat caaagcggtt
     3301 cgacggccga aaaattgact caaaccgtag gcaaggcata agagaacaat cccaaacaca
     3361 gcatggggaa gtcgtcctgt atagtccgaa tccccaaaaa ggaaaaagaa caacgcgccg
     3421 atatggtact gaaatggtcc atggtagacc ggcgtgtacc gagacggatc gcccgcgatc
     3481 tgggatgagt gaggcccata cttgaagagt cgaaacgagt agtacgcatg catggactca
     3541 tcatggtgat aagccattct tcccagatca tacagccgga aaaagccccc caacgcaatg
     3601 aggattccaa gaatcacaat tacgttggtc agtttgacgt tcttttcgag aacactcatg
     3661 gtttctttcc ttacaaagaa cagtaggatt tcaggcgact caattccgta gagattaggc
     3721 aaatcacgtt gggctgtcaa gaccgggcaa ctgcattctg tcgctttgag gatttcactg
     3781 atctgtcatt caacaatcga ggccgtctct caggaatcca gaccttggtt ggcagacagt
     3841 catttgttca gttgcacctt tccaggattc cgatacgatt agggcagtcg ttatgaggca
     3901 ttttcgcttt gagcaccgga ataggaggcc ttcgctttta tgagttgtcc cagattcgat
     3961 ttccacatgc atcccaaaca cgccggttgc gccaatgcaa ccatggaact tcctgccatc
     4021 gtccagcaat cacatagtgt tggagtggtg gccatcggta tcacggatca tctgaatcgt
     4081 cctgagcaaa taccgttgca tcggcagata cgtgatgagt tgctcacgat gcagtctccg
     4141 gtagacgtct tcttcggtgt cgaactgaac ttcacagggt gtgatcaggg attcgtattc
     4201 agcgaggaga tcaaacagga ataccagttc cagtttgcca tcggagggat tcactccaca
     4261 tatctcaatg agttcgatct cgacaaaatc atcgagatcc agcatcggca tcatctgaag
     4321 acatgcgagg attcgctggt tgacgtgttg gtgcatcctt actggtttag tcgaaaggag
     4381 ttcaaggaca agaactggcc tttcattgaa tccatgaagc ctgttcggga gtccttgaca
     4441 cgtgaactgg cgcaggcagc caaggagacg ggaaccgcca tcgagatcaa cgttggggcc
     4501 aatatccgac gccagaaaga ctatgttgaa gagtatgtgg actatctttc cattttcgct
     4561 caggaaggca cgatgttcac gataggatcg gacgctcata gcatagacca cttgctcgac
     4621 atcaaactgg cttgggatgt cgtggacaga ctcggcattc ccgaagagcg gatctggcga
     4681 cctgccaccg agccgctgct gaaggcctgt gaggtggcga agtcctgaga gaaggcggcg
     4741 tttgacggcc tcgtcccctg gtttcgttga cagggttcgg ggaagtggat atcttgactt
     4801 gtgctcatgg atactcagca gatgagtctt tacatacctt cagcagtcgt tgggatgacg
     4861 gcacttgtgg ccgcgttgct g
//
LOCUS       DAOQUC010000097         4858 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_19340_length_4858_cov_3.615865, whole genome shotgun sequence.
ACCESSION   DAOQUC010000097 DAOQUC010000000
VERSION     DAOQUC010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4858
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19340_length_4858_cov_3.615865"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(119..1138)
                     /locus_tag="PKH07_02660"
     CDS             complement(119..1138)
                     /locus_tag="PKH07_02660"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HNT33880.1"
                     /translation="MGQNQFHHDVPSRRDFIRTTVGVVGSSLAATGLLSKTSQAQPSE
                     SKSLVALSQDTKVIGAGREIAQDRMREMLDACMKQVTGESSSALAYKSLFKPRDIVGI
                     KVNCLAGRNLSSHPDLVKLIIEHLLAAAVPARNIIVWERLGRELEQAGFPLSRDGSAP
                     RCMGTDDPTVGYDAMPTESGSIGSCFSRFLSSICTALISVPILKDHDLSGVSIGMKNF
                     YGGIHNPNKYHDNNCDPYIADLNAHPFIRGKLKLTICDATTVQYHGGPSFKESWAWSY
                     GAVLVSRDPVALDRVGADLIENKRKESGLPSLKEEGREPRYIQTAATRGLGVGDSSRI
                     EVVRV"
     gene            1304..2758
                     /locus_tag="PKH07_02665"
     CDS             1304..2758
                     /locus_tag="PKH07_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019423447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNT33881.1"
                     /translation="MPSKDSRPNILFILTDDQGPWAMGCAGNDEIQTPNLDRLAATGI
                     RFDQYFCTSPVCSPARASLLTGRIPSQHGIHDWLASGNSPLEPHKDGRLVEYLRGQRG
                     YTEDLAEAGYVCGLSGKWHLGNSPQPQKGFAFWEVHAQGGGPYYNAPMIRNGQVYREP
                     RYVTDAITENALEFLQQQLISDQPFYLSVHYTAPHLPWGPEHHPKDLYDIYFNDCAFD
                     SVPDGLSAPSWVRYRNLPVDDVKTRRTYLSGYYTAVELMDANVGRLINWLEANGLREN
                     TLIVFTSDNGMCMGHHGLYGKGNATFPLCMFEESVKVPFLVSQPGNIPQGLVCSNMVS
                     HYDFRPTLLDYVGIGGGTSADLPGISFIDALSGDFSPKREGVVVFEEYGPVHMFRTQQ
                     WKYVHRYPYGPHELYNLADDPGETRNLIGDAGTREIEREMRQQLSLWFCQYVDPRRDG
                     TREAVTGSGQLGPCGPDADSYESFSQDRVRSLLP"
     gene            2809..3312
                     /locus_tag="PKH07_02670"
     CDS             2809..3312
                     /locus_tag="PKH07_02670"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HNT33882.1"
                     /translation="MKEIDVTELTCKPVDLWMNQWLILTSGTDEDCNMMTVAWGSIGC
                     MWSKPFVQIVVRPQRHTFQYLEKSDSFTLCAFPGKYRRALQTLGSISGRDGDKLSKTN
                     LTLRKSTKVSAPCYNEASLILECRIMYTQDINPAGFKDPVIEGNYPNKDYHRAYFGEI
                     VAAFIEE"
     gene            3390..4853
                     /locus_tag="PKH07_02675"
     CDS             3390..4853
                     /locus_tag="PKH07_02675"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNT33883.1"
                     /translation="MSDRPNIVFLLNDHQLHYRHGWDAGPKIQRPHFERLAAQGVEFS
                     RSYTACPLCAPARRTMLTGLFPHRHGQIRNGPDHPFDKQTYLEKLSDAGYRNFYYGKW
                     HAGPGTAHDFGCEGFSYPGYNNPYTKPEYKDYLQRRGLPEPEMLIHQNFGLTKWKPGD
                     HYIPNMPCSFGYETGILETPDDTHEAFFLANLACDQLRHLAKDKDGRPFALRVDFWGP
                     HQPYFPTQRFADLYRAPEIPEYPSFQSDLSDKPDVYRRGGNCPMTDQDGNIIHPNPIP
                     WSEWQRALALCYAHVTMVDAAGGLILDALDELGLSENTLVVWTSDHGDALGCHGGRFD
                     KGSYMPEELLRVPFAMRFPGMIPSGQVRDELVSTIDIAPTLLDAAGTGFNGSVDGESL
                     LSIAVGGRESWRESLMCETHGHFEKHFARAVIVGRHKYVENLGQMDELYDLETDPYER
                     TNLINNQACQETLSAVRKCLAEWRSTTADYDTSTDSA"
BASE COUNT         1114 a         1298 c         1295 g         1151 t
ORIGIN      
        1 ctttccagat cggcaacact acactctctc ggacacagaa cgcacttgat cttctgctcc
       61 gggagtttct cgtagtgaag tgcttcctta acgaagcgtg gatcatcgct catagctgtc
      121 ataccctgac gacttcgatt ctggaactgt cgccgacgcc cagccctcgt gtggcggcgg
      181 tctgaatata tctgggctct ctgccttcct ctttcagact gggaagcccc gattccttgc
      241 gcttgttctc aatcaggtca gcgccgaccc gatccaacgc cacgggatca cgactgacca
      301 gaactgcacc gtaagaccat gcccagcttt ccttgaatga tggaccgcca tggtactgaa
      361 cggtggtggc gtcacaaatc gtcagcttca gttttcctct gatgaacggg tgagcgttca
      421 aatcggcgat ataaggatcg cagttgttgt cgtgatactt gttggggttg tgaatgccgc
      481 cgtagaagtt cttcatccca atcgagacac cggaaaggtc atgatccttg agtatcggaa
      541 cgctgatgag tgcggtacaa atgctcgaca aaaaccgact gaaacacgag ccgatcgagc
      601 cggattccgt gggcatggca tcgtaaccca ccgtgggatc atccgtgccc atgcagcgcg
      661 gcgcagatcc gtctcgtgag agcggaaagc ctgcttgttc gagttcccgt cctaagcgct
      721 cccagacgat gatgttccga gcgggaaccg ctgcagccaa gagatgttcg atgatcagtt
      781 tgaccagatc cggatgtgag gagagatttc tgcccgcaag gcaattaact ttgatcccga
      841 cgatgtccct gggtttgaaa aggctcttgt aagccagagc agaagacgac tcgcccgtga
      901 cttgcttcat gcaggcgtcc agcatctccc gcattcggtc ttgagcgatc tcacgtcctg
      961 ctccaatgac tttggtatct tgtgagagtg cgacaagact cttgctctcg gaaggctgtg
     1021 cctgagatgt ttttgacagg agtcccgttg ctgccagtga actcccaacc actccaacgg
     1081 ttgttctgat gaaatccctt cgggacggca catcatgatg aaactggttt tgacccatac
     1141 tcaatcacct attctcattt catactatag tacgtttttg ctcgtataaa agtgcttgct
     1201 gttgctgatt ccatattcgg cttgcctgtc atcgcagggt gaaatactat ctccttgtgg
     1261 tttctcgcga agcagccaaa cggttttctg aaaggaaaca gaaatgcctt caaaagattc
     1321 ccgtcccaat atcttgttca tactcaccga tgatcaggga ccatgggcga tgggctgtgc
     1381 cggcaacgac gaaatccaaa cgccgaatct cgatcgcttg gcggcgacgg gcatacgctt
     1441 tgaccagtac ttctgcacct cgccggtctg ttctcctgcc agggcttctc tgttgaccgg
     1501 acgcattccc tcacagcacg gcatccacga ttggctggct tcgggaaact ctcctcttga
     1561 gccgcacaaa gacggcagac tcgtggaata ccttcggggg cagcgtggct ataccgaaga
     1621 tcttgcggaa gcaggttacg tctgcgggct cagtggcaag tggcatcttg gcaattcccc
     1681 gcagccgcag aaagggtttg ccttttggga agtccatgct cagggcgggg gaccttacta
     1741 caatgccccc atgattcgaa atggacaagt ctaccgggag cctcgctatg tcaccgacgc
     1801 cattactgaa aacgcactgg agtttttgca gcagcagttg atcagcgacc agcctttcta
     1861 tctgagcgtt cactacacgg caccccatct gccttgggga cccgagcatc atcccaagga
     1921 tctgtatgat atttacttca atgactgtgc cttcgattcc gttcccgacg gcctgagcgc
     1981 tccttcgtgg gttcgctaca ggaatctgcc ggtggatgac gtcaagaccc gtcgcactta
     2041 tctcagtgga tactacacgg cggtcgaatt gatggatgcc aacgtcggtc gtctcataaa
     2101 ctggcttgag gccaacgggt tgcgggagaa caccttgatt gtcttcacgt cagacaacgg
     2161 catgtgcatg ggacatcacg ggctttacgg aaagggcaac gccacgtttc cgctctgcat
     2221 gttcgaggag tcggtcaagg ttcccttttt ggtctcgcag ccgggaaaca taccgcaagg
     2281 cctggtctgt tcgaacatgg tcagccatta tgacttcagg ccaacgcttt tggattatgt
     2341 tggaattgga ggaggcactt cggcagatct tcccggcatc agtttcatag atgctctttc
     2401 gggcgatttc tctccaaagc gtgagggtgt ggtcgtcttt gaggaatatg ggcctgttca
     2461 catgttccgt acacagcagt ggaagtacgt tcatcgctac ccctatggac cccatgaact
     2521 ctacaatctg gcggatgacc caggggagac tcggaatctt atcggagatg ccggtacccg
     2581 tgagatcgaa agggagatga gacagcagtt gagtctgtgg ttctgccaat atgtagaccc
     2641 cagacgtgac ggcacacgag aggcggttac cggcagcggt cagttggggc cgtgcgggcc
     2701 cgatgcagat agctatgaat ccttctctca ggatcgggtc agatcacttc tcccgtgaag
     2761 caaactccat cattggagtc gagatacaat tcaggaaagg acgagaaaat gaaggagata
     2821 gacgtaaccg agttgacctg caaacccgtg gatttgtgga tgaaccagtg gctgatcctg
     2881 acttccggca ccgatgaaga ttgcaacatg atgactgtgg catggggcag tatcggatgc
     2941 atgtggtcaa aaccatttgt gcagatcgtc gtcaggccgc agcgccatac tttccaatat
     3001 ctggaaaaat ccgattcgtt cactctgtgc gcatttcccg gcaagtaccg tcgagcgctt
     3061 caaacgcttg gttccatttc agggcgcgat ggggacaagt tgagcaagac gaatctcacg
     3121 cttcgaaaat caaccaaggt ctccgcgccg tgctacaatg aagcctctct gattcttgaa
     3181 tgtcgcatca tgtacaccca ggatatcaac ccggccggtt tcaaagatcc tgtcattgaa
     3241 gggaactatc cgaacaagga ctaccatcgg gcttacttcg gggaaatcgt tgccgcgttc
     3301 attgaggaat aacaaacagc gctttgtgtc tctgggaaag agctgagact gccggtctgc
     3361 gtcagtccat atcctaagga gtctcttcga tgagcgacag gccgaatatt gtctttctgc
     3421 tcaacgatca tcaacttcac taccgtcacg gttgggatgc gggaccgaaa attcagaggc
     3481 ctcattttga gaggcttgct gctcaaggag tcgagttctc gcgttcctac accgcctgtc
     3541 cgctttgtgc accggcacgc cgaaccatgc tgaccggtct gtttccacac cggcacggtc
     3601 aaatccgaaa tggtcccgat catccttttg acaaacagac ctatctggaa aaactttcag
     3661 atgcgggcta tcggaatttc tactacggaa aatggcatgc cggaccggga acggctcatg
     3721 acttcggctg tgagggattc agctatcctg gatacaacaa tccttacaca aaacctgagt
     3781 acaaagacta tcttcaaagg cggggtctgc cggagcctga gatgctgatt catcagaatt
     3841 tcggtttgac caaatggaaa ccgggcgacc attacattcc caatatgccg tgcagtttcg
     3901 gctacgagac cggcattctt gagacaccgg acgacacgca tgaagcgttc tttcttgcca
     3961 atctcgcctg cgatcaactt cggcatctgg ccaaagacaa agacggcagg ccttttgcgc
     4021 ttcgcgtgga tttttgggga ccacatcagc cctatttccc gacgcagcga tttgccgatc
     4081 tctatcgtgc cccagaaatt cctgagtatc caagtttcca gagcgatctt tcagacaaac
     4141 ccgacgtcta tcgtcgtggg ggaaactgtc ccatgacaga tcaggatggg aacatcattc
     4201 accccaaccc aataccttgg agcgaatggc agcgtgccct ggctctgtgc tatgcacacg
     4261 tcacgatggt ggacgctgcg ggggggctga tccttgatgc tctggatgaa ctggggttga
     4321 gcgagaacac tctggtcgtc tggacaagcg accatggaga tgcgctcggt tgccatggcg
     4381 gacgtttcga caagggttcc tacatgcccg aagagttgtt gcgggttcct tttgcgatgc
     4441 gtttccccgg aatgatcccg tctggacagg tgcgagatga actggtcagc accattgata
     4501 tcgcgccgac gctcttggat gcagcgggta ccggtttcaa cggctctgtg gatggcgaaa
     4561 gccttctctc cattgcggtg ggaggcagag aatcctggcg tgaaagcctg atgtgcgaga
     4621 cacacggaca ctttgaaaaa cactttgcga gggctgtcat tgtaggcagg cacaagtatg
     4681 ttgagaatct cggtcagatg gatgaacttt acgatttgga gaccgacccc tatgagcgca
     4741 ccaacctcat caacaaccag gcctgccagg aaacgctgag cgccgtgaga aaatgccttg
     4801 ccgaatggcg aagtacgacg gcggactacg acaccagcac tgatagcgcc tgaaaagc
//
LOCUS       DAOQUC010000098         4820 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_19554_length_4820_cov_3.082476, whole genome shotgun sequence.
ACCESSION   DAOQUC010000098 DAOQUC010000000
VERSION     DAOQUC010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4820
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19554_length_4820_cov_3.082476"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(121..495)
                     /gene="rplR"
                     /locus_tag="PKH07_02680"
     CDS             complement(121..495)
                     /gene="rplR"
                     /locus_tag="PKH07_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HNT33884.1"
                     /translation="MPTKKELNRQKRQRRVRGRISGTPEKPRLCVFRSLRNIHAQIVD
                     DVAGRTLVACSTKCKSVASVLAETNCKPGSKEAAKRVGRCLGEQAVAAGILQVAFDRG
                     GYLYHGRVKAVAEGAREAGLTF"
     gene            complement(495..1031)
                     /gene="rplF"
                     /locus_tag="PKH07_02685"
     CDS             complement(495..1031)
                     /gene="rplF"
                     /locus_tag="PKH07_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007391733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HNT33885.1"
                     /translation="MSRVGKQPIKIPNGVSVTLDGVRVKVKGPKGSLERNIPGILSLE
                     IGSQEMSVQRPNDEWTSKSLHGLTRTLVSNMVTGVSTGFSKQLEIQGRGYRASVDGKT
                     LVLDLGFSHQIRYPIPEGMQITTPDPTKIVISGADKEAVGHLAAKIRSFRPPEPYQGK
                     GVRYVGEYVARKAGKKNV"
     gene            complement(1070..1468)
                     /gene="rpsH"
                     /locus_tag="PKH07_02690"
     CDS             complement(1070..1468)
                     /gene="rpsH"
                     /locus_tag="PKH07_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015260999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HNT33886.1"
                     /translation="MAMSDPISDLLTRIRNANLAYLERVEMPSSRMKQDILNIMREEG
                     FIKNFRLVEDRKRGVLRVYLKYGPQRERVISGLCRVSRPSLRVYVGKNEIPLVMGGLG
                     TCILSTPKGIMTGQKARRLGVGGELLCKVW"
     gene            complement(1518..1703)
                     /locus_tag="PKH07_02695"
     CDS             complement(1518..1703)
                     /locus_tag="PKH07_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012887844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HNT33887.1"
                     /translation="MAKTSLRIKATRKPKFPVRAYHRCPLCGRSRAYMRKFDMCRLCF
                     RSLAHKGEITGVTKSSW"
     gene            complement(1740..2273)
                     /gene="rplE"
                     /locus_tag="PKH07_02700"
     CDS             complement(1740..2273)
                     /gene="rplE"
                     /locus_tag="PKH07_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017310482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HNT33888.1"
                     /translation="MQVLYEDVVREALQKQFGYKNPMQMPRLVKIVLNMGIGEGSRDI
                     KILDALKGNLAQIAGQRPVTTMAKKSIANFKIRQGMPVGCSVTLRRSRMYEFLDKLIN
                     VAIPRIRDFRGLSARSFDGRGNYSLGVSEQLIFPEVEYDGIPQVQGMNITIVTTAKTD
                     EEARELLRHFGMPYRER"
     gene            complement(2534..2884)
                     /gene="rplX"
                     /locus_tag="PKH07_02705"
     CDS             complement(2534..2884)
                     /gene="rplX"
                     /locus_tag="PKH07_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005984455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HNT33889.1"
                     /translation="MAKQAVAVKDRIKQRIRRGDTVIVRKGREAGKSGKVIRVLRQKD
                     AVLVQGLNYVKKHTRPNPQIGRQGGVMEKESMIRMPNVMVVCPQCEAPTRVARKTIED
                     GKRVRECKKCSYQF"
     gene            complement(2904..3272)
                     /gene="rplN"
                     /locus_tag="PKH07_02710"
     CDS             complement(2904..3272)
                     /gene="rplN"
                     /locus_tag="PKH07_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HNT33890.1"
                     /translation="MIQPQTTLTVADNSGARKLMCIGVLGGSGKRYARIGDIIRASVK
                     EALPESNIKKGQVVKAVVVRTRKELRRKDGTYIRFDNNAAVLINEQKDPIGTRIFGPV
                     ARELREMKYMKIVSLAPEVI"
     gene            complement(3347..3604)
                     /gene="rpsQ"
                     /locus_tag="PKH07_02715"
     CDS             complement(3347..3604)
                     /gene="rpsQ"
                     /locus_tag="PKH07_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HNT33891.1"
                     /translation="MQERARRKVRVGRVVKSSMDKTVTVLIERSFPHPLYKKIVRRSS
                     RIMVHDENKKCNVGDVILVMETRPLSKNKRWRYVKTIREAI"
     gene            complement(3661..3879)
                     /gene="rpmC"
                     /locus_tag="PKH07_02720"
     CDS             complement(3661..3879)
                     /gene="rpmC"
                     /locus_tag="PKH07_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006417722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HNT33892.1"
                     /translation="MRKQVKGFRDMTLEELKSRVDSLRKNLFGLRFQLSTGQLEQTNQ
                     INALKKDIARALTVMREMEKAGEGARKA"
     gene            complement(3876..4301)
                     /gene="rplP"
                     /locus_tag="PKH07_02725"
     CDS             complement(3876..4301)
                     /gene="rplP"
                     /locus_tag="PKH07_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HNT33893.1"
                     /translation="MLMPKREKFRRQHRGRNRGKAWRGQEVSFGEYGMKAVNCGWISS
                     RQIEAARVALTRHMKRQGKVWIRIFPHKSLTEHSAETRMGGGKGAPSEHVAVVKPGAI
                     LFEVDGVTEAVAKEAMRLASHKLPVQTHFVYRDRKGVPE"
     gene            complement(4342..>4820)
                     /gene="rpsC"
                     /locus_tag="PKH07_02730"
     CDS             complement(4342..>4820)
                     /gene="rpsC"
                     /locus_tag="PKH07_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HNT33894.1"
                     /translation="VRVNIYTARPGIIIGRKGTEVERLKKALAVKTDKQVFINIKEVK
                     APDMEAQLIAENVASQLEKRISFRRAMKRPIQSALTVGALGVKICVAGRLGGAEMSRT
                     EWYREGRVPLHTLRADIDYGFATARTKAGSVGVKVWVYKGEILDLSVLRKKDEEGA"
BASE COUNT          968 a         1575 c         1053 g         1224 t
ORIGIN      
        1 ggcggtgatc ttcacacctg ccgctgcccc ggaagccaaa ctggtacaga tcggcttctt
       61 ctacaaacaa gcacgcgaat gaaccaggcc cggccgacgt gcctgctgat gctgactcgc
      121 ctagaaagtc agtcccgctt cgcgcgcacc ttccgccacg gcctttactc tgccgtggta
      181 cagatatcca ccacggtcaa acgcgacttg gaggattcct gctgcaacag cctgttcccc
      241 aagacaccga ccgacacgct tggccgcttc cttagacccc ggtttgcagt tcgtctccgc
      301 cagcacgctc gccacgctct tgcacttggt ggaacacgcc accaaggtgc gacctgcgac
      361 gtcatccacg atttgcgcat gaatgttccg cagactgcga aacacgcaca agcgaggctt
      421 ctcgggagtg ccggaaatac gacccctgac ccgccgttgc cttttttgac gattgagttc
      481 cttctttgtt ggcatcagac gttcttcttg cctgccttcc tcgcgacata ttctccgacg
      541 tatcggacgc ccttgccctg gtacggctcc ggcggtcgaa acgaacggat ttttgcagcc
      601 agatgaccca ctgcttcctt gtccgccccg gagatgacaa tcttggtcgg atccggagtg
      661 gtaatctgca ttccttccgg aatgggatat cgaatctgat gggaaaaacc aagatccaac
      721 accaaggtct tcccgtccac ggaagcacgg taccctcgcc cttggatttc caactgtttg
      781 ctgaaccctg ttgagacccc ggtcaccatg ttcgaaacca gggttcttgt caacccatgc
      841 agtgacttgg aggtccactc atcgttcggt ctctggacag acatttcctg agaaccgatc
      901 tccaaagaca aaatccccgg gatattgcgt tccaagcttc ctttgggacc cttgaccttg
      961 acgcgaaccc catccagagt aacagatact ccattgggta tcttaatcgg ttgcttgcca
     1021 actcgcgaca tctctgtctc cttagttatt gcgctcctga gcagcgctgc taccaaacct
     1081 tgcaaagaag ctcgccaccc accccaaggc gacgcgcctt ctgacccgtc atgatccctt
     1141 tcggagtgga cagaatacac gttcccaaac cacccatcac caagggaatc tcgttcttgc
     1201 ccacgtaaac tctcaaactt ggacgactca cccggcacag accactgatg acccgctcac
     1261 gttgcggccc atatttcaga tacacacgca aaacgcccct cttgcggtct tccaccaggc
     1321 ggaagttctt aatgaagccc tcctcgcgca tgatattcaa gatatcctgc ttcattcttg
     1381 aactcggcat ctccacgcgc tccaaatatg ccaggttcgc gtttcgaatg cgcgtcaaga
     1441 ggtcagatat cggatcactc atcgccattg tctcttcctc cggcctgttg ccctcgtcct
     1501 aactgggtac tgccctgtca ccaacttgac tttgtaacgc ccgtaatctc gcctttgtgc
     1561 gcgagcgacc tgaagcaaag gcggcacata tcaaacttcc tcatgtacgc tcgggaccga
     1621 ccacacaggg ggcaccgatg gtacgctcga accggaaact tcggctttct tgtcgctttg
     1681 atgcgcaggc ttgtctttgc cactttctgc cttcctccct tacccttggg aacgctcggt
     1741 tatctctctc ggtacggcat tccgaaatga cgaagcagct cccgcgcctc ctcgtcagtc
     1801 tttgccgttg tcacaatcgt gatattcatc ccctgcactt ggggaattcc gtcatactcc
     1861 acttccggga agatcaactg ctcggacact cccagactgt agttgccacg tccatcaaat
     1921 gaccgcgccg aaagacctcg aaagtctcga atcctgggaa tcgccacatt gatcaacttg
     1981 tccagaaact cgtacatccg actgcggcgc agtgtcacac tgcatcccac aggcattccc
     2041 tggcggattt tgaagttcgc aatggacttc ttggccatag tcgtcaccgg tctctgacct
     2101 gcaatctgag ccaggtttcc cttcagggca tccagaatct tgatgtctct ggagccttct
     2161 ccgattccca tattcagaac gatcttcacc agccgcggca tctgcatcgg attcttgtag
     2221 ccgaattgct tctgaagagc ctctcgaacg acgtcttcgt aaagcacctg caatcggggc
     2281 ttctgcctcg gctgagcggc ctgctgttcc tgcttttctt tctttccgtc cttagccacc
     2341 ttagctccct ccgaaccgga ttcggccaga cctcctcgcg ggaatcagag tcccctgggg
     2401 tctttctcac tcattttctt tgcgggactg gaaatcaatc ctcacacccg cagttactaa
     2461 ctttctttcc ttgtgcatcc attccacgcc acggcagcaa aagattcact cccaggcgtt
     2521 tactcctgga cgctcagaac tgatacgagc actttttgca ctcgcgaact cgcttaccat
     2581 cctcaatcgt ttttcgagcc actcgcgtcg gggcctcgca ctgagggcag accaccatca
     2641 cgttcggcat tcgaatcatg gattcctttt ccatcacacc gccctgccga ccaatttggg
     2701 ggttgggtcg ggtgtgcttt ttcacgtagt tcagcccctg aaccagcacc gcatccttct
     2761 ggcgcagaac acgaatcacc ttgccgctct ttcccgcttc gcggcctttg cgaactatta
     2821 cagtatcgcc acggcgaatt cgctgcttga tcctgtcctt cactgccact gcctgcttgg
     2881 ccattgtccc gtcccttcct gctctatatc acttcgggag ccagcgagac gatcttcatg
     2941 tacttcatct ctctgagttc cctggccacc ggtccaaaga tacgagtgcc gatgggatcc
     3001 ttctgttcat tgataagaac cgccgcgttg ttgtcgaatc gaatgtaagt tccatccttg
     3061 cgcctgagtt ccttacgggt tctcaccaca acggctttga cgacctgtcc cttcttaatg
     3121 ttgctctcag gcagagcctc tttgacagaa gcgcggatga tatccccgat acgggcatac
     3181 cgcttgccac ttccgcccag aacgccgatg cacatcagct tacgcgcccc tgagttgtcc
     3241 gccacagtga gtgttgtctg aggctgaatc actgtcttgt ctcctcgctc cgtttaatca
     3301 agactcgccg agtcctcacc caaaagagac ccctcttggc atccgttcag atggcctctc
     3361 gaatcgtttt gacatagcgc catcgcttgt tctttgacag agggcgcgtc tccatgacga
     3421 gaatcacgtc accaacattg catttcttgt tctcgtcgtg aaccataatc cggctcgatc
     3481 gtcgaacaat cttcttgtaa agtggatgcg gaaaagaccg ctcgatgaga accgtcaccg
     3541 tcttatccat gctcgactta accactctgc caactcggac ttttcttcgt gctctctctt
     3601 gcatctttta ttctcccgcg tctcatccct caaggggtat gcctgaacat cccttcgggc
     3661 tcaggccttc cgtgccccct cgccggcctt ctccatctcc cgcatgaccg tcaaagcgcg
     3721 ggctatatcc ttcttgagcg cattgatctg attggtctgc tccaactgcc ccgtcgaaag
     3781 ttggaaacga agaccaaaaa ggttcttccg caaactatcc actcggctct tcaactcttc
     3841 caaggtcatg tcccgaaaac cttttacctg ttttctcatt ctgggacccc cttacgatct
     3901 cgatacacaa agtgcgtctg aaccggaagt ttgtgacttg ccaaacgcat ggcctctttc
     3961 gccactgctt cggtcacgcc atcaacctcg aaaagaatcg ctcccggctt cacgactgca
     4021 acgtgctcgg acggagcacc ttttccacca cccattcgag tctccgctga gtgttctgtc
     4081 agagacttgt gtgggaaaat acgaatccaa accttcccct gccttttcat gtgacgagtc
     4141 aacgccacac gagccgcctc aatctgtcgg ctggagatcc agccacagtt gactgctttc
     4201 atgccatact cgccgaagct cacttcttgg ccgcgccagg ccttgcccct gttgcgtccc
     4261 cgatgctgtc ggcggaattt ctcccttttc ggcatcaaca tgattcaggt tcctccggaa
     4321 tcctatcgtc ctgctcttgt ctcaggctcc ctcttcgtct ttctttcgaa gcacggacag
     4381 gtcaagtatt tctcccttgt agacccagac cttcactcca acgctaccgg ccttcgttcg
     4441 cgccgttgcg aatccatagt caatatccgc ccgcaacgtg tgtagcggaa cacgtccctc
     4501 tcggtaccat tctgtccgac tcatctcggc tccaccaaga cgacccgcaa cacaaatctt
     4561 gacacccaac gccccgacag tcagcgccga ctgaatcggt cgcttcatcg cccgacggaa
     4621 ggaaatcctc ttctccaact ggctggcaac attctcggca ataagttgcg cttccatatc
     4681 cggagccttg acttccttga tgttgatgaa aacctgcttg tccgtcttga cagcaagggc
     4741 tttcttgagt ctctcgactt cggttccctt tcgaccaatg atgatcccgg gacgcgccgt
     4801 gtagatattc accctgactt
//
LOCUS       DAOQUC010000099         4798 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_19688_length_4798_cov_3.731815, whole genome shotgun sequence.
ACCESSION   DAOQUC010000099 DAOQUC010000000
VERSION     DAOQUC010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4798
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19688_length_4798_cov_3.731815"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1439
                     /locus_tag="PKH07_02735"
     CDS             <1..1439
                     /locus_tag="PKH07_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004074674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNT33895.1"
                     /translation="YDIIEESRDYRPGLSEGMKYYCPEYLPAIKDGLMRCLATGEPFA
                     IEAEVITGKGNRRWVEVRGLTRADSGQRPMVVGTIQDITERKQAEQERAQLTDQLQQA
                     QKMEFVGRLAGGVAHDFNNMLMVIGGNLELALSQMDSHDPKYVPLVEAHTAAQRSADL
                     TRQLLAFARKQTIARKVLDLNETVAGMLKMLQRLIGENITLNWKPDPNLWKVNMDPSQ
                     VDQMLANLCVNARDAISDVGVITIQTRNASIDEQYCAKNLDAVAGQYICLSMSDSGCG
                     MAPETLQHIFEPFFTTKEVGSGTGLGLATIYGIVKQNQGFIEVKTEVGQGTTFDIYLP
                     RYVDKAESVLLAEGEAPKAEPGHEVILVVEDEEAILRLITMMLKRQGYTVLAANTPGE
                     AMQLAREYPGDIHLLITDIIMPEMNGRALTKNLLSLYPGLKSLFVSGYTADVIAQHGV
                     IEEGVNFIQKPFSAEVLTARVREILKEE"
     gene            1580..>4798
                     /locus_tag="PKH07_02740"
     CDS             1580..>4798
                     /locus_tag="PKH07_02740"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNT33896.1"
                     /translation="MRSVSNKTFGSWKSREIIELPVAGGINLLLWMVWAPALMSMSQT
                     FVRRLFRCARRCPLTVGLLLAVLCPPSTSYATPTHIVVGTGNAFPPVVFLDENGQPTG
                     RDIDLWRLWSEKTKVSVEFRLTEWSEVIPALLRGEIDVADGASYTPERAKLLAFTQPY
                     QELQAFAFFDENTRPVSRLMDMEGMTVGVLRGGNVEESLRQEVPGADLMPLSDYRALV
                     EALRQGQINVLVAEEPMISYHFNQTGGARGFRRSSLPFFRGELRMAVRKTDKDLLALV
                     RRGHAQITKKERQSIDVKWFGVGWAESRATPARLVLLFASVIASVAVVMTVAFGRRWL
                     KDQPNITAARNSLVNLWLVFLALTSPLFLLSSLARALESPWTPLLTMHVCIAPIFWLL
                     TLFRHHLAYSIRVFSLLGILFLLGLGGLLTYGLFGMTAIVFFVFVIVATTLFGVRGSV
                     LSITLSLLSIGSVGLAVGTGFWNYSFREEAYAVSTTFWAFAFVGFGLWSLVATLGIGG
                     VYRHMEDALTRQKQSEERFRLLAMHLPVLIDAITKEGQFTFWNRACEETTGYSQSEVL
                     GNPQAYAHLYPDPDYRNQLTREWEESGRVFKNKETVLRTKTGQQRCILWSNLPMELSF
                     SESDSWAVGIDITERKNAEQALRESESKYRSLFDQSVEGIYLHDLEGRILDVNQMACQ
                     QSGYTREELLQLTVFNGHPDKAEMQDILQQWQQWEPGQRVTIEAQHRRKDGTLYPVEI
                     STGLVTHKGMNLMLAIVRDITERVRAEEALRDSQERVKLLSDNIPNSMVYQVVVGEDT
                     AARTFTYLSAGVEQLHGITAAEGMRDAFAIYNQVLEEDRGMLLRREMDCLAAMSTFRA
                     EFRIQKPSGEVRWVMASSSPRKLAENLVVWDGLEIDITERKRAEEALRNSVSLLNASL
                     DSTADGILVVNREGKVTLWNRKFAELWRLSDELLATHDDATLLAAVLSQLKHPEQFLD
                     QVRALYASPEATSFDTLDFLDGRVFERYSQPQKIGDETVGRAWSFRDITARKHAEKEQ
                     ERLQNQLAQAQKMESIGRLAGGVAHDFNNMLTVISGHTAMALE"
BASE COUNT         1150 a         1195 c         1350 g         1103 t
ORIGIN      
        1 tctatgacat cattgaggaa tcacgagact atcgtccggg cctcagcgag ggcatgaagt
       61 actactgtcc cgagtatctt cccgcgatta aggacgggct gatgagatgt ctggcaaccg
      121 gagaaccttt cgcgatcgaa gccgaggtca tcacgggaaa gggaaacaga cgctgggtcg
      181 aagtgcgcgg tctgacgaga gccgatagcg gacagaggcc tatggtcgtc ggaacaatcc
      241 aagacatcac cgagcgcaaa caggcggagc aggaaagggc tcagctgaca gatcagctgc
      301 agcaggcgca gaagatggag tttgtcggca ggctcgctgg aggcgtggcg cacgacttca
      361 acaacatgtt gatggtcatt ggggggaacc ttgaactggc attgtctcag atggactccc
      421 atgatcctaa gtatgttccg cttgttgaag cgcacaccgc agcacagcgc tctgcggact
      481 tgacacgcca actgctggcg tttgcccgca agcaaacgat cgcgcgcaag gtgctggatc
      541 tgaacgaaac cgtggccgga atgcttaaga tgctccagcg actgatcggg gagaacatta
      601 ccctcaactg gaagcccgat cccaatctct ggaaggtcaa tatggatcca tctcaagttg
      661 accagatgct tgccaatctc tgcgtcaatg cccgtgatgc tatctctgac gtcggagtga
      721 tcaccatcca gacgaggaat gcttccattg acgagcagta ctgtgccaag aacctggatg
      781 cggttgcagg tcagtatatt tgtttgtcca tgagcgactc tggctgtggc atggcgccgg
      841 aaacactgca acacatcttt gagccgttct ttacgacaaa ggaagtcggt tcaggaaccg
      901 gactgggact tgctacgata tacggcattg tgaagcagaa ccaagggttc attgaggtaa
      961 agaccgaagt cggacaaggc acaacattcg acatctatct gccccgttat gtggacaagg
     1021 ctgagtcggt attgcttgcg gaaggagaag caccgaaagc agaacccggt cacgaggtaa
     1081 tactggttgt tgaagatgaa gaggcaatac tccgattgat cacgatgatg ctcaaaagac
     1141 aggggtacac ggtgctggcc gccaacactc ctggggaagc catgcaactc gccagagaat
     1201 acccaggaga tatccacctg ttgatcaccg acatcatcat gccggagatg aacggcaggg
     1261 cactcaccaa gaatcttctc tccctttacc cgggtctgaa gtctctattt gtttccggct
     1321 acacggcaga tgtgatcgcg cagcacggtg tgattgaaga gggtgtcaat ttcatccaga
     1381 agccgttttc tgcggaggtt ctgacagcga gagttcgaga gattctgaag gaggaataga
     1441 cagatctttt tcgattccgg tgggattgtt gtccggaaac cattcagata ctatcatgaa
     1501 gatgtatgtt ggaaatagct ggctgactgg gttgtacacg aactcggcag aatgggactg
     1561 agatgatagg gatgcaccaa tgagatcagt ctccaataag acgttcggca gttggaaatc
     1621 gagagagatc atcgaattgc cggttgccgg aggaatcaac ctgttgctgt ggatggtttg
     1681 ggcaccagca ttgatgtcca tgagccagac ttttgttcga cggttgtttc gttgtgccag
     1741 aagatgtccg ctgaccgttg gactgctctt ggcggttctg tgccccccat ccacatctta
     1801 cgccacgcca acacatatcg ttgtcggaac aggcaacgct ttcccgcctg ttgtgtttct
     1861 ggacgaaaac ggtcagccta cagggcggga tatcgatctt tggcgcctct ggtctgagaa
     1921 aactaaggtc tcagtggagt ttcggctgac cgagtggtcg gaagtcattc ccgcattgct
     1981 cagaggtgaa atagacgtgg ctgatggggc ttcatacact cctgaacgtg caaaactctt
     2041 ggcattcacc cagccctatc aggaactaca ggccttcgct ttctttgatg agaacacccg
     2101 acctgtgagc cgtttgatgg acatggaagg gatgaccgtc ggtgtcctgc ggggcggcaa
     2161 tgtcgaggaa tcactccgac aagaagtgcc aggcgccgat ctcatgccat tgagtgacta
     2221 ccgagctctg gtagaggcat tgcgacaggg acagatcaac gtcctggtgg cagaagagcc
     2281 gatgatctca taccacttca atcagacggg tggagcaaga ggctttcgac gctcttcact
     2341 tcctttcttt cgtggggaac ttcgcatggc ggtccgcaag acagacaaag accttcttgc
     2401 tcttgtgaga agaggtcatg cgcaaatcac gaagaaagaa aggcagagca ttgatgtaaa
     2461 atggtttggt gtcggttggg ctgaaagccg agccacaccg gcacgacttg tgttgctttt
     2521 tgcatctgtg atcgcatctg ttgctgtcgt gatgactgtt gcctttggcc gaaggtggct
     2581 caaggatcaa ccgaacatca ccgcggcaag aaacagtctc gtcaacctgt ggctggtctt
     2641 tctcgcgctt acctctccgc ttttcctgct ctcttctctg gcgagagcac tggagtctcc
     2701 gtggactcct ctgctgacga tgcacgtctg cattgccccg attttctggc tcttgaccct
     2761 ctttcgtcat cacctggcct attcaatccg tgtcttctcc cttctcggaa tactctttct
     2821 gctcggtttg ggtggtcttc tcacttacgg actgtttgga atgacggcca tcgtcttctt
     2881 cgtgttcgtc atcgttgcca caacgctgtt cggagtgcgt ggcagtgttc tctcgatcac
     2941 actgagtctg ctttccatcg gtagcgtggg tctggctgtt ggcacgggtt tctggaacta
     3001 ctcctttcgt gaagaggctt atgcagtgtc cacgaccttt tgggcatttg cctttgtcgg
     3061 gtttggtttg tggtctcttg tggcaacgct cggaattggt ggggtttaca gacacatgga
     3121 agatgccctc accagacaga agcagagcga ggagcgcttc cgactactcg ccatgcactt
     3181 gcccgtcttg atcgatgcca tcaccaaaga gggtcagttc actttctgga acagggcatg
     3241 cgaagaaact accggctaca gccaaagtga agtgctcgga aatcctcaag cctacgcaca
     3301 tttgtatccc gatccggact atcgcaatca gttgacgcgt gaatgggagg aaagcggcag
     3361 agtcttcaag aataaagaga ctgtcctcag aaccaagacg ggacagcaac gatgcatcct
     3421 ctggtcgaat ctgccgatgg aactttcctt ttccgagagc gactcgtggg ctgtcggcat
     3481 tgacatcacc gaacgcaaaa atgcggaaca agccctgcgt gaaagcgaga gcaaataccg
     3541 ttcgcttttt gaccagtctg tggaaggtat ctatctccat gaccttgaag gaagaattct
     3601 ggacgtcaat caaatggcgt gtcagcagtc cggatacacg agagaagaac tgttgcaatt
     3661 gacggtattc aatggccatc ctgacaaggc agagatgcaa gacattctac agcaatggca
     3721 gcagtgggag ccaggacaaa gagttaccat cgaagcacag catcggcgca aagatggaac
     3781 gctctatccg gtggagattt ctacaggcct tgtcacacac aaaggcatga acctcatgct
     3841 ggccatcgtg cgtgatatca ctgagcgtgt gcgtgccgaa gaggcgttac gagacagcca
     3901 ggaaagagtg aaactgctaa gtgacaacat acccaacagc atggtctatc aagtggtggt
     3961 tggtgaggat acagcggcgc gcaccttcac gtatctgagt gcgggagtgg aacagcttca
     4021 tgggattacg gctgctgaag ggatgcgtga cgccttcgct atctataacc aggtgttgga
     4081 agaagaccgc ggcatgcttc tccggcggga aatggactgc ctggccgcaa tgtctacgtt
     4141 tcgagcggag ttccgtatcc agaaaccgtc tggagaagtt cgttgggtga tggcttcctc
     4201 gtcacctcga aaactcgctg aaaatcttgt ggtatgggat ggtcttgaga tagacatcac
     4261 ggagcgcaaa cgcgctgaag aagccctgag aaactctgtg tcgctcctga acgcctcact
     4321 ggattccacc gccgatggta tcctggttgt gaacagggaa gggaaagtga cactctggaa
     4381 caggaagttt gccgagttgt ggaggctctc agatgaactc ctggcgacgc atgacgatgc
     4441 aacgcttctt gccgccgttc tgtctcagct gaagcatcct gaacaatttc tcgaccaagt
     4501 cagagcgctc tatgccagcc ctgaagcgac aagtttcgac acgctcgatt tcctcgacgg
     4561 gcgcgtgttc gaacgatact cccagccaca aaaaatcgga gatgagacgg ttggtcgtgc
     4621 atggtctttt cgagatatca ccgcccgaaa acatgccgaa aaggagcagg aaaggcttca
     4681 aaatcaactg gcacaagctc agaagatgga gtccatcggc cggctggccg gtggtgtggc
     4741 tcacgacttc aacaacatgc tgaccgtgat cagtgggcat actgcgatgg cgctggag
//
LOCUS       DAOQUC010000100         4774 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_19829_length_4774_cov_2.855902, whole genome shotgun sequence.
ACCESSION   DAOQUC010000100 DAOQUC010000000
VERSION     DAOQUC010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4774)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4774)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4774
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19829_length_4774_cov_2.855902"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2020)
                     /locus_tag="PKH07_02745"
     CDS             complement(<1..2020)
                     /locus_tag="PKH07_02745"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HNT33897.1"
                     /translation="MLEHLEAGRWEKHPCFEVKGFGIEERVAFVKEQVESASSSRILT
                     ATTTLPLFMEYSGAKSDASPELSISGRSIPFMNRGGTTDRMQGEFWDVLGNAPETVVP
                     LSTFSRDASTERIAEGESLVLKAGESATAEITLSTEWSHIALLGLARGEGREPVRFEV
                     FLDGSSIGARTIRLAVSNSFDFICQARPGMHLLRIFVHADKQDRSLHLNGCLIQPLSN
                     KIVFSADESTPLSDHASANIRYQTYAPSSALREQVSRDYWLLRRRDSLDLSMRERAEI
                     SLQTRSALFTPPDSRFVFSVKEIPRHAVIRFGIAAVIGQVDENSSVRFELAFLHSSGS
                     LFSLFSRSLNPAERWHDCSVDLAGLAGASGSFLMDTTSDSVKSGMEVNAWWSNPSIWV
                     PSQAGSPPRNIILISWDTVRADHMSIYGYDRNTTPYLCSAVDDWVVFQNAISPGSWTL
                     PVHASVFTGLNASTHGVTMPNNRLLPGQPTIGHYLRSTGLFTGGLVRNVLVSQAYGFD
                     DGFCEYSHLRQFSAEEISESASSWIDKHSDLPFFLFLHYFDAHFPYSPPAQYDLFGDP
                     EYAGDIDGDARFQDFIDGKKTAEDISAEDLAEVVSLYDGEILRLDAALQALVQRLMDL
                     SLLDDTMIVLFSDHGEEFAEHGGFAHGPKLHEEVIRVPLLVKPWKGDRS"
     gene            complement(2354..4087)
                     /locus_tag="PKH07_02750"
     CDS             complement(2354..4087)
                     /locus_tag="PKH07_02750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33898.1"
                     /translation="MNVRSENSVPWKTFFLVSLLSILPIYLFGYWLPGRGEFLGLIRN
                     SGDASQYLALARQYQDHCLLVARNPFTHEPHSPFMIQPFLSLIGLICRITSLPCHVVY
                     AIASVFLGTALLATAWQFCRVLFPRKSESFWAWMFLTFTGGANSLLLVKHLLVGGYLI
                     DGPYQPMVVDTNNLFNVLVGVPFRASFYILIFKSWTMILQMRLVVERSEELDCGLSLP
                     CPTDAEHKPDHTSVKRNRRSLLLAGSLLLIVGFCHAYDAPYLILSLVVWTGLNIFFSQ
                     TSTRRERYRDGFFLLLCTVPWLIPPIVHALYVVTIHPVGRTIAEIPQPTPGLIPFLWW
                     IHGLSSIGIILWLGFEVFQSPLRRERSRFLFAMFVAAVLLRSLPAHYAGRLAEPFDYI
                     ALLMGISGWFRLLRCRVFSKMLFSERRRNTFFCVLFLISIPITVFCYAQRYIQIARPS
                     FDRLIYHLQSSEMRMIEWIDRNIESDEAILASRDVSLHIPRLTGCSVYVGHPVLTPDF
                     YLKREFLSLFFDTKFARRDRETFLKKNGIRYVFYGQRERLLGNANLLLSPSFRVVHRE
                     GDTYLLEFADR"
     gene            complement(4153..>4774)
                     /gene="sppA"
                     /locus_tag="PKH07_02755"
     CDS             complement(4153..>4774)
                     /gene="sppA"
                     /locus_tag="PKH07_02755"
                     /inference="COORDINATES: protein motif:HMM:NF013506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HNT33899.1"
                     /translation="NVPVVACLMDVGASGGYYVALAADRIFAHPTTITGSVGVIAQMV
                     HVHGLFEKIGVSTDTIKSGALKDAGSPFRSLTDEERNLMQGIIDQLYGRFIDTVQARR
                     PGLSPERLEQISTGRVFSASDALENGLIDSVGYLDDALEEAKKLAAIRKAHVVMYRKP
                     FSYRGNIYARVQTLPTQSGDFNLFKMDASNLALLNGRPVFLYLWQP"
BASE COUNT         1307 a         1251 c         1183 g         1033 t
ORIGIN      
        1 ggcttctatc ccccttccaa ggcttgacga gaagagggac acggatgacc tcctcatgca
       61 gtttgggtcc gtgagcgaat cccccgtgct ctgcaaactc ctcgccatgg tcagaaaaga
      121 gcacgatcat ggtatcatca agcaatgaca gatccatcag tcgctgcacc agcgcctgca
      181 gggctgcgtc caatctgaga atctctccat catagagcga aactacttct gccagatcct
      241 ctgcggaaat atcctcagcg gtcttcttcc catcaatgaa gtcttggaat cgagcgtctc
      301 catcaatatc ccccgcatac tccgggtctc caaaaaggtc atattgcgcg ggtggcgaat
      361 aagggaaatg tgcatcaaaa taatgcagaa acaggaagaa gggcaggtct gaatgcttgt
      421 ctatccatga agacgcactt tcggaaatct cttcggcaga aaactgtctc aggtgactgt
      481 actcgcagaa gccatcatca aaaccatagg cttgtgaaac aaggacattc ctgacaagcc
      541 caccggtgaa cagacctgta gaacggagat agtgcccaat cgtgggttgc cctggaagaa
      601 gacgattgtt gggcattgtc acgccatgtg tcgaagcgtt gagacccgta aagactgaag
      661 catgcacggg taatgtccat gaacctgggc tgatggcatt ttggaagaca acccaatcgt
      721 ccacagcgct gcacaagtat ggagtcgtat tccgatcata tccatagatg ctcatgtgat
      781 ctgctcggac ggtgtcccaa gagatcagaa tgatattccg tggaggggaa ccggcttggg
      841 atggcaccca gatagatgga ttcgaccacc atgcattcac ttccattcca ctcttcacgc
      901 tatcagatgt tgtgtccatc agaaaacttc cagatgcccc cgcaaggcca gcgaggtcaa
      961 ccgaacaatc gtgccaccgc tcagcaggat tgagacttct tgagaacaga ctgaacaaag
     1021 acccgcttga atgcaggaag gccagttcaa atcgcaccga ggaattctcg tccacttgtc
     1081 caataactgc cgcaatgcca aagcggatta cagcatgccg aggaatctct ttgaccgaaa
     1141 aaacaaacct ggagtctggt ggcgtaaaga gggcggatct tgtctgaagg gagatctcag
     1201 cgcgttccct catactcaga tcaagtgaat cccttcggcg caagagccag tagtcgcggc
     1261 tgacttgttc cctcaacgct gaagaaggag cgtaggtttg atagcgaata ttggcacttg
     1321 cgtgatctga aagaggcgtt gactcatccg cagagaagac gatcttattg gacagtggct
     1381 ggatcagaca tccgttgaga tgcaaagacc ggtcctgttt gtcggcatga acgaatatcc
     1441 tcaagagatg cattcccgga cgtgcctggc agatgaaatc aaatgaattg gagacagcaa
     1501 gacgaattgt tctcgctcca atacttgatc cgtcaaggaa tacctcgaaa cgaacgggct
     1561 ctcgcccttc tccccttgca agacccagga gggctatgtg agaccattcc gtggagagtg
     1621 tgatttctgc agtggctgat tcacctgcct tcaaaaccag ggactctcct tcagcaatcc
     1681 gttctgttga tgcatctctt gagaacgtgg aaagaggcac aacagtttca ggagcgttcc
     1741 ccagaacatc ccagaactcg ccctgcattc tatccgtagt tcctcccctg ttcataaacg
     1801 gtattgaccg acctgaaatg ctcagttcgg gggatgcatc cgatttggca ccgctgtact
     1861 ccataaacaa ggggagagtg gtggttgcag tcaagattcg agaacttgaa gcagactcga
     1921 cttgttcctt aacgaatgca actctctctt cgatgccgaa ccccttaacc tcaaaacagg
     1981 ggtgtttctc ccaacgccct gcctcgagat gctccagcat cgcaagatgt agttgccgtc
     2041 tggttgggga ctcattcacc ctgcacgcta cagaaaatcc catgatcacg gcagctagac
     2101 acaacactct cagcgaagaa cactctgaaa tcttgaaaag gcaatgcgat agaaaggtgt
     2161 attgccgctc acttctcagt ggaactgtcg gtccaccaag accttttccc gtccggcgac
     2221 tgtcgaagaa gcgatatctc attttgacct gcgcatccag agtaagaaat gtctccgagc
     2281 tgaaaccatg cgtactcttg tatcctttgg ctaagtcatt ttagtcatcg gtgtgaccat
     2341 agcactctcc atcctatctg tccgcaaact caagaaggta cgtatcacct tcacggtgca
     2401 caactcgaaa tgatggcgaa aggagaagat tggcatttcc gagaagtcgt tccctttgtc
     2461 cgtagaaaac atatctgatt ccattcttct taaggaatgt ctcccgatca cggcgtgcaa
     2521 actttgtatc aaaaaacagc gaaagaaact cccgtttcaa atagaaatca ggggtgagta
     2581 ccggatgacc gacatataca gaacaccctg tgagccgagg aatgtgcaga gagacatctc
     2641 gagaagcgag gatcgcttcg tcgctctcaa tgttcctatc tatccattcg atcatcctca
     2701 tctcactgct ctgcagatgg tagatgagtc ggtcgaatga tggtcgggca atctggatgt
     2761 aacgctgtgc atagcagaat accgttattg ggatcgagat cagaaagagc acacaaaaga
     2821 atgtgttccg ccgccgttca gaaaacagca tctttgagaa gacacgacac cggagcagtc
     2881 tgaaccaacc cgatatcccc atcagcaagg caatgtagtc gaaaggctcc gcgagacgtc
     2941 ctgcatagtg tgccggcaag gatcgaagca gcacggcagc aacaaacatc gcgaaaagaa
     3001 atctgctcct ctccctgcgg agcggtgact gaaatacctc gaacccgagc cacaggatga
     3061 taccgattga cgaaagtcca tgtatccacc agagaaaggg gattagtccg ggagtgggct
     3121 gggggatttc cgcaatcgtc ctgccgacgg gatgaatggt aacaacataa agagcatgga
     3181 cgatgggcgg gatcagccat ggaaccgtac acagcagcaa gaagaatccg tcgcggtacc
     3241 tttcccgacg ggttgacgtc tgactgaaga aaatgttcaa gcctgtccac acaacaagag
     3301 agagaattag gtatggggca tcatacgcgt gacaaaaacc aacgattaga aggaggctgc
     3361 cggcaagcaa tagactccgc cgattccgtt tcacacttgt gtggtctggt ttgtgctcag
     3421 catccgtcgg gcacggcaat gagagaccgc agtccaactc ctccgatctc tcaactacca
     3481 agcgcatttg cagaatcatc gtccacgatt tgaaaatcaa gatatagaaa ctcgctctga
     3541 aaggaacacc gacgagcacg ttgaaaaggt tgttggtgtc aacaaccatt ggctgatagg
     3601 gaccgtctat cagataccct ccaacgagta gatgcttcac cagcaacagg ctgttcgctc
     3661 ctccagtaaa cgtcaggaac atccacgccc agaatgactc gctcttgcga ggaaacagaa
     3721 cacgacagaa ctgccaggcg gttgccaaca gcgccgtgcc caggaagact gatgcaatgg
     3781 catagacaac atgacagggg agtgacgtaa tccggcagat caggccgata agagagagaa
     3841 acggttggat catgaatgga ctatggggct catgcgtgaa ggggttacga gcgactagaa
     3901 gacaatgatc ctggtactgc cgagccaacg caaggtactg actcgcatca cccgaattgc
     3961 gtatcagtcc caggaactct cccctcccag gaagccaata tccaaagaga tatatcggca
     4021 gaatggaaag caaagagact aagaagaacg tcttccaggg aacactgttc tcggatcgaa
     4081 cgttcaaagt ctcacctctc tctcgatgcc accacagttg ggcgattcac gatccttcct
     4141 ctgaatcttc atctagggct gccacagata gaggaatacg ggtcttccgt tgagcagagc
     4201 caggtttgaa gcgtccatct tgaacagatt gaagtcccca gattgcgtcg gcaatgtctg
     4261 gacgcgcgca tagatgttgc cccgatatga gaaaggtttt cggtacatca caacatgagc
     4321 cttcctgatc gcagccagct tcttggcttc ctcaagcgcg tcatcgaggt atccaactga
     4381 atcaatgaga ccattctcca aagcatctga ggcgctgaat actcttccgg tcgagatctg
     4441 ttcgagtcgt tctggggaaa gaccaggacg ccgtgcctga acggtatcaa taaagcgtcc
     4501 gtacaactgg tcaatgattc cctgcatcaa gttcctctct tcgtcagtca aggatcgaaa
     4561 tggagaaccc gcatctttca atgctccgga cttgatggtg tcagtgctca caccgatctt
     4621 ctcaaagaga ccgtgcacat gaaccatttg agcgatcacg ccaacgctgc cagttatggt
     4681 tgtcgggtga gcaaagatcc gatcggccgc caaggccacg tagtaccccc cggaagcgcc
     4741 aacatccatc aggcaagcaa ccacgggtac attc
//
LOCUS       DAOQUC010000101         4771 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_19846_length_4771_cov_3.264207, whole genome shotgun sequence.
ACCESSION   DAOQUC010000101 DAOQUC010000000
VERSION     DAOQUC010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4771)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4771)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4771
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19846_length_4771_cov_3.264207"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1016
                     /locus_tag="PKH07_02760"
     CDS             <1..1016
                     /locus_tag="PKH07_02760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33900.1"
                     /translation="SPLDPEQMTSQQRRRWLDFCEWQQNVLPDFARKIAEIVLEFYPA
                     EKVKIKPGGSAGGINPLSWGTNCPGFAKTLSGLAIGAQPADAHGAVFGDKWAYTAYRH
                     YGIPFSSETAGGIEYNDQVRRIFSDASAGAIQLFTYEYDKHGPVVQKYIHLYQGKYPK
                     TTTAVYAPITDFRFGRSLAPTIHASQKLRDVTDFDVLDELLIRDGFLSRYTHLVLFQA
                     EWMESDVMERIQNWQKQGGVTIAFLDRQTRLIEGQDAAPYSVCLPASDCRGEASLLSL
                     YEKLPPSLDGKPDGLWITEYLDGSVLLYNDTNQSIYRTIEHAGGSRRILVEAHTIAAI
                     PAN"
     gene            complement(1049..2464)
                     /locus_tag="PKH07_02765"
     CDS             complement(1049..2464)
                     /locus_tag="PKH07_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019598523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BNR-4 repeat-containing protein"
                     /protein_id="HNT33901.1"
                     /translation="MMLLKLFGVFLVVLVCSAALAQEGDTTDEPILINAKDDGYRGIW
                     YYNQASNDEYVYKYSGGLGTYCANHIPFCWYVPEVSKTFFTYGGTTKDSNRKLIHMVS
                     YYDHMTGTVPRPTILLDKKTSDAHDNPVINVDDKGYIWIFSSSHGTGRPSYISRSTKP
                     YSIDQFELIWTGNYSYPQPWYMPGKGFLFIHIWYGVGGRSIQMMKSADGKNWTDRELL
                     SYIGVGHYEISAPFKDKVGVAFNYHPTGLGLNYRTNLYYMESPDFGETWKSADGTVLE
                     TPLNEIVNPALVKDYEHHDPRLNVYTIDINYDKDGNPIILYITSLGYQSGPANMPRTW
                     TTARWTGSEWDINGSIVSDNNYDFGSLHIESDNLWRIIGPTETGPQPYNPGGEIALWE
                     STDKGKTWNKVRQMTTGSEYNHTYVRRPVNAHPGFYGFWADGHGRQPSDSRLYFCNDK
                     GDVYRLPVSMTAEFEIPVKIN"
     gene            2826..3503
                     /locus_tag="PKH07_02770"
     CDS             2826..3503
                     /locus_tag="PKH07_02770"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HNT33902.1"
                     /translation="MKEIILAIALFLAFTRLASATETSPLPRVLIIGDSISIGYTEPL
                     QEILKDRALVTHNPGNAQHSAFGLENLDDWLGNVKWDVIHFNHGLHDLKYVDKNGKNS
                     KTKENAHIQIPLDEYRKNMEAIVTRLKKTGAVLIFATTTPYPEGVSPLRIPADVKKYN
                     AAALKIMKKQGVVVNDLYTFILPQLSSLQKPTNVHFTAEGSKALAQEVARHILQAIGQ
                     QGTPVSN"
     gene            complement(3686..4402)
                     /locus_tag="PKH07_02775"
     CDS             complement(3686..4402)
                     /locus_tag="PKH07_02775"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S double cluster binding domain-containing
                     protein"
                     /protein_id="HNT33903.1"
                     /translation="MDSRALKDEIRQIAPSFGADLVGFVDITTQAGNLTKAGFTAHEQ
                     YECAISIGIPVINSIADQIGTTTTASAAYLYRLFCYDIINNRLNEIAFSIASHLEKAG
                     FRSFPVPSTHLATANRIQPGLFSHKATAYLAGLGWIGKSCLLVTPQFGPRLRLTTVLT
                     TAQIGDDPKPMENRCGECTRCLEICPAKAFTGREFAITDPLEVRMDAAKCDEHLKKKE
                     ETVGVRVCGLCVTVCPYGNA"
BASE COUNT         1124 a         1251 c         1243 g         1153 t
ORIGIN      
        1 ccagtcctct tgatccagag cagatgacga gccaacagcg tcgccggtgg ctggattttt
       61 gcgaatggca gcagaatgtt ctgcctgatt tcgcccgcaa gattgccgag attgtcctcg
      121 agttctaccc cgcggagaaa gtcaagatca aaccaggtgg cagcgcggga gggatcaatc
      181 ctctgagttg gggaaccaac tgcccgggat tcgccaagac actttctggt ctggcgattg
      241 gtgcacagcc cgcagatgcc catggcgctg tgtttgggga caagtgggcg tacacggcat
      301 accgacacta cgggattccg ttttcctcgg agaccgccgg gggaattgag tacaacgatc
      361 aggtgcggcg cattttcagc gatgcttctg ctggggcgat tcaactgttc acctatgaat
      421 atgacaagca cggtcccgtc gtccagaagt acattcatct ctatcagggc aagtatccga
      481 aaacgacgac ggcggtctat gctccgatca cggatttccg attcgggaga tctttggcac
      541 ctacaattca tgcctctcaa aaactgcgcg acgtgaccga tttcgatgtg cttgacgagc
      601 tgctgatacg cgacggattc ctgtcgcgct acacgcatct ggttttattc caagccgaat
      661 ggatggagtc cgatgtgatg gagcgcattc agaattggca aaagcagggg ggagtgacaa
      721 ttgcgtttct cgacagacag acacgcctta tagagggtca ggacgctgcg ccttactcgg
      781 tctgtcttcc tgcgtccgat tgccgtggcg aagcgtcgtt gctctctctc tatgagaaac
      841 tcccaccaag tcttgacgga aagccagacg gtctctggat cacagaatac ctcgatggat
      901 cggttctgct ttacaacgac accaaccagt ccatctatcg aaccatcgaa cacgccggcg
      961 gatcccgacg aattcttgta gaagcacaca cgatagccgc gattcctgcg aactaagcgt
     1021 gcggaacagg acggtcgccg tgttctcgtc agttgatctt caccggtatt tcaaactccg
     1081 cggtcatact gacaggaagt cggtacacat cacccttgtc gttgcagaag tagagcctgg
     1141 agtcggacgg ctgtcgtcca tggccatccg cccagaaacc gtagaagcca gggtgtgcat
     1201 tgaccggcct tcgaacataa gtgtgattgt actcactgcc cgtcgtcatc tgtcggactt
     1261 tgttccaggt cttgcccttg tcggtgcttt cccacagagc gatctcaccg cctgggttgt
     1321 agggctgcgg accggtctct gtcggcccaa tgattcgcca caggttgtcg ctctcaatgt
     1381 gcagtgaacc gaagtcgtag ttgttgtccg agactattga gccgttgatg tcccactcgg
     1441 agccagtcca tcgcgccgtc gtccaagtcc tgggcatgtt ggcgggaccg gactgatagc
     1501 ccagactggt gatgtaaagg atgatcgggt ttccatcctt gtcgtagttg atgtctatgg
     1561 tgtagacgtt gagccgagga tcatggtgct cgtagtcctt caccaatgca gggttgacga
     1621 tctcattcag tggggtttcc agtacggtac catcggcact cttccatgtc tcgccaaagt
     1681 ctggtgattc catgtagtac aggttggttc tgtagttcag tccgagtcct gtcggatggt
     1741 agttgaacgc aacgccaact ttgtctttga acggagcact aatctcgtaa tgacccacac
     1801 cgatgtagga gagaagttcc ctgtccgtcc aattcttgcc atcggcgctt ttcatcatct
     1861 ggatacttcg accaccaaca ccgtaccaga tgtgaatgaa cagaaagccc tttccgggca
     1921 tgtaccacgg ttggggatag gaatagttgc ctgtccaaat caactcgaat tgatcgattg
     1981 aataaggctt tgtgcttctt gagatataag agggtctgcc ggtaccgtgg ctgctggaga
     2041 agatccagat gtagcccttg tcatccacat tgatcacagg attgtcatgg gcgtcactgg
     2101 tctttttgtc cagaagaatc gttggtctgg gcacggtgcc tgtcatgtga tcgtagtagg
     2161 acaccatgtg aatgagtttt ctgttggaat cctttgtggt tcctccatac gtaaagaagg
     2221 tcttgctgac ttccggcaca taccagcaga aaggaatgtg attggcacag taagttccca
     2281 gaccgccgct gtatttgtag acatactcat cgttggatgc ctggttgtag taccagatgc
     2341 ctcgatagcc atcatccttg gcattgatca agattggctc atctgttgtg tccccttctt
     2401 gtgccagcgc tgcactgcat accaacacca caagaaaaac accaaacaac ttcagcaaca
     2461 tcataggggc acctctctca ataggatcac tgctttcgat gcgtagtgaa gacatcctat
     2521 cagctgccga caaaactgca agaccttctg tcgccaaggg atttgcgaca gaccttgtac
     2581 agtgctgcag atgatatctg ccctgcttcg gcagcgtgca atcgcggaca cgattgacaa
     2641 catgcccaaa cctgctcata tttgctttga gttcttaggc cgtcacggac agaaaagaga
     2701 ctacagatcg gacggccgac caaaagagac ggaacaaggc accgagagag tcatagattt
     2761 accatctatc cgacagctcg tagcagcaga gtccggtgtt gaacacagaa agaggacaga
     2821 tagacatgaa agaaataatc ctggccatcg cattattcct ggcgttcacg cgtctcgcct
     2881 cagcaacaga gacgagtcct ctgccacgcg tcttgatcat cggagactca atatccatcg
     2941 gatacacaga acctcttcag gagattctca aggatagagc ccttgtcacc cacaacccgg
     3001 gaaatgctca gcattccgcg ttcggactcg aaaatctgga tgactggttg ggcaacgtaa
     3061 aatgggatgt cattcatttc aatcatgggc tgcacgatct gaagtacgtg gacaaaaacg
     3121 gcaagaactc caaaacgaag gagaatgccc atatccaaat ccctcttgat gaataccgga
     3181 agaatatgga agccatcgtc acccgactca agaaaacagg ggcagtcctg atctttgcga
     3241 caacgacacc ctatcctgaa ggcgtgagcc ctttacgcat tccagcggat gtgaagaaat
     3301 acaacgcagc agccttgaag ataatgaaga agcagggtgt tgtcgtcaat gatctgtaca
     3361 cgtttattct gcctcagctc tcctcacttc agaaacctac gaacgtccat ttcacggcag
     3421 agggatcgaa ggctctcgcc caggaagtgg cgagacacat tctgcaggcg attggtcaac
     3481 aaggcacgcc ggtgagcaac tgattgccgc caccgtttcc tgacggataa taccttacgg
     3541 cgtcgctaca aatccgacgc tgcatgaatg ctgccctacg gatcccgggc tccgcactcg
     3601 cttcttctga aaagcttcat acgacatgga aagtctccaa tgtgggcgtt ggccgcaagg
     3661 ccccgatttg tgggagagat gggcttcagg cattgccgta gggacagaca gtgacacaca
     3721 ggccacatac cctgacacca acagtttctt ccttcttctt cagatgttcg tcgcacttcg
     3781 cggcgtccat cctgacctca agcggatcag tgatggcgaa ttcccttccg gtgaatgctt
     3841 ttgctgggca gatttcaagg catcgtgtgc attctccgca acgattctcc atcggctttg
     3901 gatcatcccc gatctgagca gttgttagaa cggtggtcag acgcagacgc ggtccgaatt
     3961 gtggtgttac gagaagacag cttttcccga tccagccaag tccagcaagg tatgccgtgg
     4021 ctttgtgtga gaataatccc ggttgtattc tattcgcagt ggcaagatgt gtggagggga
     4081 ctggaaacga tctaaatccc gccttttcca aatgggatgc gatggagaaa gcgatctcgt
     4141 tcaatcggtt gtttatgatg tcatagcaga acaagcgata cagatatgcg gcggatgccg
     4201 tggtagttgt tccgatttga tcggcgattg aattgatgac gggaatgcca atgctaatcg
     4261 cgcattcata ttgctcgtgg gcagtgaagc ctgcctttgt gagatttccc gcctgtgttg
     4321 tgatatcaac aaacccaaca agatctgcgc cgaatgaggg ggcgatctgt cttatctcat
     4381 ctttcagggc tcgagaatcc atatttttac cctctgtgcc tattgtagaa gtcaaggctg
     4441 gctccgcaga tccatcctac aggccgtgtg ccagccttca ggttcgcgaa gcggttctga
     4501 gggctgctgc tctatcttgc atccatgaac tcaccatgat gccaagtctc tcatatttga
     4561 ggcgtatggt caaatgcacc tcggaggatg gacacccggc cccttcagga ttgtggcgtg
     4621 ggcttccagc ctgcaatctt agagaagcct ttcgcaggca agatgcctgc gccacaacta
     4681 caaggctatg aagtgctgca ggtggggaca cccacgcccc ttgccctcat cccgaccttt
     4741 ttcgagaaga tctcctggga acgccaagtg t
//
LOCUS       DAOQUC010000102         4755 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_19933_length_4755_cov_3.712979, whole genome shotgun sequence.
ACCESSION   DAOQUC010000102 DAOQUC010000000
VERSION     DAOQUC010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4755)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4755)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4755
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19933_length_4755_cov_3.712979"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..653)
                     /locus_tag="PKH07_02780"
     CDS             complement(<1..653)
                     /locus_tag="PKH07_02780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33904.1"
                     /translation="MYHWRHTTLYLASFMFLTGRAHSADLPTLEATLGANGVVQEGAW
                     SGAQVRLRTRNDPFEGNLRLVHQKRGFPEVVIVKPVEMPRFNIQDISLQIALGVPLFE
                     SLSLELENKGRILLTQPLAWRLSALERKTHDPVPSDLLNMERKAFAVRMPPSGNWQRD
                     LAFVFCSGACFALFVIATKRRWKTGVRRILALVGLLLLVYSSGVGRATAKNSAFEVL"
     gene            complement(754..2781)
                     /locus_tag="PKH07_02785"
     CDS             complement(754..2781)
                     /locus_tag="PKH07_02785"
                     /inference="COORDINATES: protein motif:HMM:NF024909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA and WFA domain-containing protein"
                     /protein_id="HNT33905.1"
                     /translation="MFFFIQNPWVLLGLAGTAVPILLHLQKRRSAKTVRFPSLQLLSS
                     IHRTKRVSLRVRELLLLLIRTALILFLILGLSGIELERRGVVTPQSLSRQTVPDQVIL
                     IDTSYSMAYLESGIPRLQHAIDAAAEILQRRRAGQRFLVLPFSEGVDARDLENAQWTS
                     DVERCLDRMREWQPCGQGTDVTKTLRQVSELIVDRARSRIWFFSDLQRTGWLEALGTL
                     RQESAFRERLPETLVFPVGEMDTRNLWLEFPPKGVPRFLARAEGFPLPLVARSEGYGH
                     SQTIAGTLQLHGQLNLTGSEPVACSVYDRRLQTQGDEQRRVEVPLVPLGSLAISPEPS
                     TVRRPIFCFWKGEAVFDVDSGSEELDRLAFDNSIRWSVPVLWSLPVVLIRSDAPTVAS
                     RVLEACLSPMSDENSFFVKMTSMPVSSVSLEKLESFSVAVLQEGVWASMAPAAQDSLL
                     RYVREGGKLIAFTPEQESFGLIASVSATAELVDTVSGTLALCDVNWSHPALMPLEGFG
                     AESLMGVTVYRAAQLGGLDQNWLKIRHVSTPASQEQRAIGDPMPVLGEIQYGKGNLVF
                     SGIGLEGARSDLASSVVFLPLIHQALKFLVSDQAPDAAEDSHLSGERAESRLESLSRE
                     ERQQAAQDTGLLIVEEGIPLVGRRRRDLTVPLLMLVLGLALAELWIGNRQL"
     gene            complement(2784..3470)
                     /locus_tag="PKH07_02790"
     CDS             complement(2784..3470)
                     /locus_tag="PKH07_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33906.1"
                     /translation="MEIPSQSDQGNRVLIELRAWPAVKNTLVRYYDDLWRLIAANLLW
                     FTISIPIVTLPAATIGLCRYCERLLVYDDPSLATILTSGFKRFLLRAYVFALVFLLTV
                     VATGFSVFFYVKQAEQFGFIMLALGSLAICFTLFFLMCSIYVLPFMVHQDIGLGLAIK
                     RSALLAGAKPLVTFLLLLLDGLLVGIVVQFRALPLILFVPSLSLLKNTTALLLALGEM
                     EIGTEPKPKD"
     gene            complement(3478..3942)
                     /locus_tag="PKH07_02795"
     CDS             complement(3478..3942)
                     /locus_tag="PKH07_02795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33907.1"
                     /translation="MRSICTLMILVLLVCTACKGKPPDQKVDASTQNERVTTVEESPE
                     PAVRNLAGIQLPDGFPDDVFVADGAEVTAVVHDNDQYQVDILVDGELEGLASLWESKM
                     QTSGWLVREKIVTEQKAQMKFAKASGKRGALVDMQAGKPLNNVRLTVSELKE"
     gene            complement(4041..4421)
                     /locus_tag="PKH07_02800"
     CDS             complement(4041..4421)
                     /locus_tag="PKH07_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011937523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HNT33908.1"
                     /translation="MNAEAVQWLHYAVENLRTAELCLENELYNPCIQNAQQSIEKALK
                     ALCLSVPIALRKTHSIEGLRNDLRGAGIECNLTDEECFLLDSVYLPSKYPLGSVLPDF
                     LPDRDTAEQCLLIARKALRHLFAE"
     gene            complement(4411..4710)
                     /locus_tag="PKH07_02805"
     CDS             complement(4411..4710)
                     /locus_tag="PKH07_02805"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HNT33909.1"
                     /translation="MTEDTKSEITTEIARCLKEAKEIRKIVVFGSFLTSDCPADVDVA
                     VFQDSDEDYLSLAMKYRSLSKSVSDRIPLDVIPLRRDAKGFLVEEINKGKVIYER"
BASE COUNT         1194 a         1351 c         1191 g         1019 t
ORIGIN      
        1 tccagcactt cgaaagccga attctttgcg gttgctctgc caaccccaga ggagtaaaca
       61 agcagcaaga ggccaaccag agccaggatt cttctcacac cggttttcca tcgcctcttg
      121 gtcgcgataa caaacagggc aaaacacgca ccagagcaaa acacaaatgc cagatccctc
      181 tgccagtttc cagatggtgg catccgcact gcaaacgcct ttcgttccat attcagaaga
      241 tcggagggaa cgggatcatg ggtctttcgt tcgagtgctg acaaacgcca tgccagaggc
      301 tgggtcagaa gaatccgtcc tttgttctcc aattcaagac tgagcgattc aaacagaggc
      361 acaccaagag caatctgaag agagatgtcc tggatattga agcgaggcat ctcaactggc
      421 ttgacgatga ccacttcagg aaaccctctc ttctgatgaa cgagacgcaa atttccctca
      481 aagggatcat ttctcgtgcg aagtcgcacc tgcgccccgg accaagcccc ttcctgcact
      541 accccgttgg cgccaagagt tgcttccaat gttggcaagt cagcggaatg tgcccgacct
      601 gtcagaaaca taaaggatgc aaggtagagt gtcgtatgtc gccagtgata catcgttatg
      661 tagagcacct cgagatcagg tctttccgtc tgtcactccg caggagatgg gacacctgat
      721 ttgccgtctt actcccgtcg aggggagatc cttctacaac tgccggttcc cgatccagag
      781 ttcagccagg gcaagaccca agacgagcat cagcagaggt acggtcagat ctcttcgacg
      841 tcggcctacc aagggaatac cctcttccac aatcagcagt cccgtgtcct gtgccgcctg
      901 ttgtctctcc tctctgctga gactctcaag gcgcgattcc gcccgctcgc ctgacaagtg
      961 ggaatcctca gcggcatctg gcgcttgatc tgatacaaga aacttcagcg cttgatgaat
     1021 caggggcaga aagaccacag aagaagccag atcagatctg gctccttcca aaccgattcc
     1081 cgagaatacg agattacctt tcccgtattg tatctcgcca aggacaggca tggggtcacc
     1141 gatcgctctc tgttcctggg atgcgggagt tgagacatgc cgaatcttca accagttctg
     1201 atcgagtccg cccagttggg cggcacggta caccgtcact cccatgagag actctgctcc
     1261 aaatccttcc aagggcatca gagcaggatg tgaccagttg acatcgcaca gagccagcgt
     1321 tccggaaacc gtatccacta actccgctgt ggcactgact gaagcaatca gtccaaagga
     1381 ttcctgttcg ggtgtgaacg cgatcagttt gcctccttcg cgaacgtaac gcagaaggct
     1441 gtcctgtgcc gccggtgcca tactcgccca cactccttcc tgaagcacgg ccacggagaa
     1501 ggactccaac ttttcaaggc tcactgaaga gacaggcatt gacgtcatct tgacgaaaaa
     1561 cgaattctcg tccgacatgg gtgacagaca tgcttccaag actcgggaag ccaccgttgg
     1621 cgcatcagat cgaatcaaga ccaccggaag cgaccacagc acgggaaccg accaccgaat
     1681 cgaattgtca aatgccagcc gatccagttc ctcggaacca gagtccacat caaaaactgc
     1741 ctcacccttc cagaaacaga agatgggtct tctgaccgtc gaaggctctg ggctgatagc
     1801 gagcgatccc agaggcacca gcggaacttc aacgcgtcgc tgttcgtcac cctgtgtttg
     1861 aagacgccga tcatagacgg aacaggcaac aggttcgctg ccagtgaggt ttagttgtcc
     1921 gtgcagttgg agtgttcccg cgattgtctg tgagtggccg tagccttccg aacgtgccac
     1981 cagaggcagc ggaaatccct ctgcccttgc aagaaatcgc ggaacgccct tggggggaaa
     2041 ctccagccaa aggtttcgag tgtccatttc cccgaccgga aagacaagcg tttcgggcaa
     2101 cctctcccga aatgcactct cttgacgcaa tgtgccaaga gcttcgagcc aacccgtgcg
     2161 ctgcaagtca gaaaagaacc agatgcggct tcttgctctg tccacgatca attcagaaac
     2221 ctgacgaagt gtcttcgtaa catcggtacc ttgaccgcag ggctgccatt cccgcatccg
     2281 atccagacat cgctccacgt ccgaagtcca ttgggcattc tccaaatcac gggcatccac
     2341 cccttcggaa aaaggaagaa ccagaaaccg ttgccctgct cgtcttctct ggagaatctc
     2401 cgcagcggca tctatggcat gctgcaagcg tgggattccc gactccagat acgccatgct
     2461 gtaggaggta tcaatcagaa tcacctgatc gggaactgtc tgtcgggaca gactctgcgg
     2521 agtgaccaca cctcgccgtt ccaactcgat cccggacagt cccagaatga gaaacaggat
     2581 caatgctgtc cgaatcaaga gcagcagcaa ttctcgaacg cgcagactga cgcgttttgt
     2641 gcgatggatg cttgaaagca gttggagtga aggaaatcga accgtctttg ccgatcgtct
     2701 cttctgaaga tgcagcagga tcggtacggc agttcccgcc agtcccaaaa gcacccaggg
     2761 gttttgaatg aagaagaaca tgctcagtct ttcggtttcg gctccgtgcc gatttccatt
     2821 tctcccagag ccagaagcag cgccgtggtg tttttcagca ggctcagact cggcacaaag
     2881 agaatcaacg gcaacgcccg gaactgtacc acgatcccga ccagaagacc atccagcagc
     2941 aagagtagaa acgtcaccag aggtttggcg ccagccaaaa gcgccgaacg cttgatagcc
     3001 aaacccagac cgatatcctg atgcaccatg aagggcagca cgtagatgga gcacatcaga
     3061 aagaacagcg tgaaacagat cgccagcgag ccgagtgcca gcatgatgaa tccaaattgc
     3121 tcggcctgct tcacatagaa gaagacactg aaacccgtgg ccaccaccgt cagaagaaag
     3181 accaacgcaa agacgtatgc ccgaagcaag aaccgcttga agccggatgt gagaatcgta
     3241 gccaaagacg gatcatcata caccaacagc cgctcgcaat aacggcacaa gccaatggtc
     3301 gctgcaggaa gggtcacaat cgggatgctt atggtgaacc agaggagatt ggctgcgatc
     3361 agtcgccaga gatcgtcata gtagcgtacc agggtattct tgaccgcagg ccatgctctg
     3421 agttcgatca acacgcggtt gccctgatct gactgagaag ggatttccat cgagtcctta
     3481 ttccttgagt tccgagaccg tgagtctgac gttgttgagt ggctttcctg cttgcatatc
     3541 caccagagcg cccctcttgc ctgaggcttt ggcgaatttc atctgggcct tctgttccgt
     3601 aacgatcttc tcccgaacga gccaccctga agtctgcatc ttgctttccc acaacgacgc
     3661 aagaccttcc agttcgccgt caaccagaat atctacctga tattgatcat tgtcatgaac
     3721 gacagcagtc acttcggcac catcggcaac aaaaacgtca tcggggaacc cgtcgggcag
     3781 ttggatgccg gcgagattgc gaacagccgg ttcaggagac tcctcgaccg tggtgactcg
     3841 ctcattctga gtggatgcat ccactttctg atcgggtggt ttgcccttgc aggcggtgca
     3901 aaccagcaga accagaatca tcagtgtgca tatgcttctc atcgaaaatc tcctccaaac
     3961 ggatacttct cgccgtgaac gaggatccct gctatctgtt tatcgtgtcc ctcagtagct
     4021 ttgactccag aagaggcgtg tcattcagcg aacaagtgac ggagcgcttt cctggctatg
     4081 agaaggcatt gctccgcagt atcccggtct ggcagaaagt cagggagcac cgaacccaac
     4141 gggtacttcg agggtagata cacagagtcc aagaggaagc actcctcgtc ggtcaggttg
     4201 cattcgatgc cagcaccgcg aaggtcgttt cttaatcctt ctatactgtg cgttttcctc
     4261 agggctattg gaacactaag acatagagcc ttcagcgcct tctcgataga ctgctgggca
     4321 ttctggatac atgggttgta gagctcattt tcgagacaca gttccgcagt acgaaggttc
     4381 tcaacggcgt agtgaagcca ctgaactgcc tcagcgttca tagatcacct tacccttgtt
     4441 gatttcttcg acaagaaaac ccttcgcgtc acgacgcaaa gggatgacgt ccaacgggat
     4501 gcgatcggag accgactttg agagagaacg atacttcatt gccaaagata gataatcttc
     4561 gtcgctgtct tggaaaacag caacatccac gtctgcagga cagtcgctcg tcagaaaaga
     4621 cccaaagaca acgatctttc tgatctcctt ggcttctttc aggcaccgtg ctatttcggt
     4681 tgtgatctca gacttggtgt cttccgtcat ggttcacatc tccgacagaa tactgtcttc
     4741 atcatgagtt cctat
//
LOCUS       DAOQUC010000103         4753 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_19938_length_4753_cov_3.104087, whole genome shotgun sequence.
ACCESSION   DAOQUC010000103 DAOQUC010000000
VERSION     DAOQUC010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4753
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19938_length_4753_cov_3.104087"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..426
                     /locus_tag="PKH07_02810"
                     /pseudo
     CDS             1..426
                     /locus_tag="PKH07_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003226808.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
     gene            721..1233
                     /locus_tag="PKH07_02815"
     CDS             721..1233
                     /locus_tag="PKH07_02815"
                     /inference="COORDINATES: protein motif:HMM:NF013177.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33910.1"
                     /translation="METFEEDQTPLLDQISPEKEAEETKSEVRVTELLEAEEIEQLLR
                     KLEKSDLPVTLLRDKGNGSLEERRPLFIAKSDDQETPCYSLLGLLEAVKKAGRKGLYI
                     QRYKGLGEMNADQLWETTMNPEKRVLMQVTLDDLAQAEEIFTILMGDSVEPRRDFIQE
                     HAPQVRFLDI"
     gene            complement(1617..2201)
                     /locus_tag="PKH07_02820"
     CDS             complement(1617..2201)
                     /locus_tag="PKH07_02820"
                     /inference="COORDINATES: protein motif:HMM:TIGR01656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNT33911.1"
                     /translation="MKTPGEQGRPAVFLDRDGTIIEDLGDLSQPSQVVFFDDTFQALR
                     RLAPFFALFIVTNQSGVAKGSISIQDVHRVNSYVQSRLAEEGVPIVATYVCPHKRDEG
                     CCCIKPSPYFLNKAACEHGIDLHRSYVIGDHPHDVVLASHAGARGIYLLTGHGMQHRD
                     EIPESASVASGIREAAELILQELKRSERLRESNA"
     gene            complement(2198..4084)
                     /locus_tag="PKH07_02825"
     CDS             complement(2198..4084)
                     /locus_tag="PKH07_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4914 family protein"
                     /protein_id="HNT33912.1"
                     /translation="MEETKQTWHILTPPAELRKILSASPRVHVAGSVDELFELACGGA
                     GSDFVEVSYRLPSGESFVEATEARVRNGIAVNYTEVYMRRRDPDTMVISDDLPSDKPR
                     FQERFGKPFSSLRQQTFDWLAQQELAVFAFRSGWRDLGAQALAVAPANAAFFPLGLAL
                     LQGIVPLEEIPQDFSPSVVIYVAPVFRHTFFDGKQVVVHNRGGDVYELFSYNLYPGPS
                     AKKGVYGTLIHLGEEQGWVAAHCSGVQAVTPYDNVVTFMHEGASGGGKSEMLEQPHRM
                     PDGQLLKGYNIITGERRYIEIQRTCDLCPVCDDMALCHPSIQQDNGKLWIVDAEEGWF
                     VRVNHIDSYGTDPNLEKLTAQPSRPLLFLNIDAVPGSRALIWEHTDDQPGVPCPNPRV
                     ILPRSMVPNVINEPISVDIRSFGIRTPPCTLESPTYGIIGLLHVLPPALAWLWRLVAP
                     RGHANPSIVASEAMSSEGVGSYWPFASGRQVRQANLLLEQIQQTPHTRHVLVPNQYVG
                     AWHMGFMPQWLTRDYLARRGSAKFKGDQIVPARCSLLGYALSSMRIEGVRVAHWFLEV
                     NTQPEVGNEGYDKGAAILREFFCKHLVSFLEPDLSDLGRMIIECCLQDGTVSDYEQLL
                     GKSE"
BASE COUNT         1176 a         1253 c         1280 g         1044 t
ORIGIN      
        1 cccgggaaac tggcggactg ttccgagcgc gacccttcgc tgtgcgagat atatctggtt
       61 gagggagact cggcaggcgg gattgccaag atggggcgtg accgacggtt tcaggcgatt
      121 cttccactga agggaaagat catcaacgtc gagaaggcgc gtctcgacaa ggtgctttcc
      181 aacgaggaaa tccagacgat catcaccgcc ctgggaacgg gtgtcggtga agatgacttc
      241 gatgtcagca agtctcgcta tcacaaggtc atcatcatga cagatgcgga tgtggacggt
      301 gcgcacattc gaacgcttct gttgacattc ttctttagac aaatgtaccg actggtcgaa
      361 cagggatatg tctatatcgc gcaacctcct ctgtttctgg ttcgcaaagg caagtccatt
      421 cagtacattg acaaggaaga aaaccttgac gcgcatctta tggaactggg cttgcagggg
      481 acgtcggcag tggttacggg taacggttcg caacgcgaac tcactggaac gcagtttcgc
      541 gatctgatca acaggctcta tgaactggaa agccttgagc gtgtcctttt gaagaaaaac
      601 attgaactcg atgacttcct gaatctgcgc gaccgttcca agggaggact tcctctctat
      661 caggtcgaga tagacggtga gatccagtac ttctattccg agaaggacta ctcaaagatg
      721 atggagacgt ttgaggagga tcagacgcct cttctggatc agatcagtcc tgagaaggaa
      781 gccgaggaga ccaagagtga ggtgcgtgtc acggaactcc tggaggcgga agaaatcgaa
      841 cagttgcttc gcaaactgga gaaaagtgat ctccccgtca ccctgctccg tgacaagggc
      901 aatggctcgc ttgaagaaag aagaccactg ttcatcgcca aaagtgatga ccaggaaacg
      961 ccatgctaca gtcttcttgg tctgctggaa gcggtcaaga aagcgggacg caaggggctt
     1021 tatatccaac gttacaaagg tctgggtgag atgaacgcag accagctctg ggaaacaacc
     1081 atgaatccag agaaacgagt gttgatgcag gtcacacttg atgatctggc gcaggctgag
     1141 gaaatcttca cgatcctgat gggcgattcc gttgagccaa gacgcgactt catccaggaa
     1201 cacgccccac aggtgcgatt cctggatatc taggccaact tcagcggttt ggggcgtaat
     1261 tgtttctgaa agagatttcg gaacgtggga aggagttgtt ttttgcgtgt tctgatgtca
     1321 tttaatgtca atcgcgggat gtgtggcgtt gtgttgtgag tctgaacgct gagaagtggg
     1381 tgtgttgaaa aagagaggcc agaagtttca atgaatgttc tggactcctt ggattcaacc
     1441 gctcagcccg caggaaccat aacccggttg aagattgaaa tcagagcacc gaagatcagt
     1501 tacgtctggg tagtgctgag atcacaggac tctcaaagcc ggaacaaaga ctgaccctgt
     1561 gtagagaaca ccaagactgc gcggcagcag catgaatgca acaaacagag catacttcat
     1621 gcgttgctct ccctcaatcg ctcggagcgt ttcaactcct gaagaatcag ttcagcggct
     1681 tctcggatgc ccgaggcaac gcttgcggat tcgggaatct cgtcacgatg ctgcatccca
     1741 tgacccgtga gaagatagat accccgagcg cctgcatgag aggccaggac cacgtcgtgg
     1801 ggatgatcgc caattacata ggagcggtgg agatcgattc cgtgttcaca cgccgccttg
     1861 ttgaggaagt aaggacttgg cttgatgcag caacaaccct catccctttt gtggggacag
     1921 acatacgtgg caacgattgg cactccctct tcagcaagtc ggctctgcac gtatgagttg
     1981 actcggtgga catcttgtat ggagatcgat cctttggcaa cgccggactg gttggtcaca
     2041 ataaacagcg caaagaacgg agccaaccgc cggagggcct gaaaagtatc atcgaaaaag
     2101 acgacttgag acggctgaga caaatcaccg aggtcttcga tgattgtccc atcccggtcg
     2161 agaaacacgg ccggacgccc ctgctctcca ggtgttttca ttcggatttc ccaagcagtt
     2221 gctcatagtc tgatacggtg ccgtcctgca gacagcactc tataatcatg cgacccaaat
     2281 ctgataaatc cggctctaag aaagatacca gatgcttgca gaagaactct ctgagaattg
     2341 ctgcgccctt gtcgtagcct tcattgccga cttctggctg cgtgttgacc tccagaaacc
     2401 aatgcgccac acgaacccct tcgatgcgca tcgagctgag cgcatagccg agcaatgagc
     2461 aacgggcggg gacaatctga tcccctttga acttggcact tccgcgtctg gcaagatagt
     2521 cgcgagtcag ccactgaggc atgaaaccca tgtgccaagc gccgacgtac tggtttggaa
     2581 caagcacatg tctggtgtgc ggggtttgtt gaatctgctc aagaagcaga ttcgcctgcc
     2641 tgacttgccg ccccgacgca aagggccaat atgaacccac tccttcgctg ctcatcgctt
     2701 ccgaagccac gatactggga tttgcatgac cccgaggcgc gacgagacgc caaagccaag
     2761 ccagagcggg tggcaagaca tgcaacaagc caatgatgcc ataggttgga ctctcaaggg
     2821 tgcatggggg tgttcttatt ccgaatgagc gaatatcaac actgatcggt tcgttgatca
     2881 cattgggcac catcgaacgg ggaaggatga cacgagggtt cggacaggga actcctggtt
     2941 ggtcgtcggt atgctcccaa atcaaggctc gactccctgg cactgcgtca atgttcagaa
     3001 acaacagtgg gcgggatggt tgtgccgtca gcttctcgag gttcggatct gttccgtagc
     3061 tgtctatgtg attgacacgg acaaaccatc cctcttccgc gtcaacaatc caaagtttgc
     3121 cgttgtcctg ctgaatgctg gggtgacaca acgccatatc gtcacaaacg ggacacaaat
     3181 cacacgtgcg ctggatttct atgtagcgcc tctccccagt gatgatgttg taccctttga
     3241 gcagctggcc atcgggcatc cggtgcggtt gctcgagcat ttcactcttg cctcctccgc
     3301 tcgccccctc atgcatgaag gtcacaacat tgtcgtaagg cgtgactgcc tgtaccccgg
     3361 aacagtgcgc agccacccaa ccttgctctt cacccaggtg aatcaaagtc ccataaaccc
     3421 ctttctttgc acttgggccc gggtagagat tgtaggagaa caactcgtaa acatcaccac
     3481 cgcggttgtg gaccacaacc tgtttgccgt cgaaaaacgt atgccgaaac acaggagcta
     3541 cgtagattac aacactcgga gagaaatcct gtggtatctc ctccaacggg acgatcccct
     3601 gcaacagagc gagacctaaa gggaagaatg ccgcattcgc cggcgcaacc gccagagcct
     3661 gcgcccccag gtccctccaa ccggatcgaa aagcgaaaac tgccaactcc tgctgtgcca
     3721 gccagtcgaa cgtctgctga cgcaaactgg aaaacggttt tccaaaacgt tcctgaaatc
     3781 ggggcttgtc cgatggcaga tcgtcagaga tcaccattgt gtctggatct cgtcgccgca
     3841 tatagacctc ggtgtaattg accgcgatac cattgcggac tctggcttca gtcgcttcga
     3901 cgaaactctc tccacttggg agacgatagg acacctccac gaaatctgaa ccagctcccc
     3961 cacaagccag ttcgaacagc tcgtctacgc tcccagctac atgtacccgc ggtgaggcgc
     4021 ttaggatctt cctcaactca gccggcggtg tgagaatatg ccacgtctgt tttgtttctt
     4081 ccatcgtatc tgactccagg tgctgaccca aatctgcacg tcagcgattc gccatcgtac
     4141 accttcggac ggattcggat gaccgctgct acggcagtcc aaggactcac tgttctgtat
     4201 gtttcgtctc tgctgtgaaa gcctcctctc ctgttccaag atgggagagc acttggacgg
     4261 gcctacgcct tgtttctgat cccctctcac ttccccttaa cagcgaaaac cgcaagatcc
     4321 ttccgcagca gaaaaaactc ctaacgctca accgtcctgc caatgccacc aattggacgg
     4381 ttgaccgcta gcagttgcga ggaagtatgc tcagttttgc tttcttctgt caagagactt
     4441 caggccagac ctttcccgga aatttggagg ggtttgaagg ataggggatt gaggatactg
     4501 ggttacatat agtcggggat aggctctgag agaaacgggg aaagtccccg cagttgctgg
     4561 acggtttgga tgtgaaaggc agccccttcg cgacaggcgc aagagatgtt catactatgt
     4621 catagcgcct caaggcggag gaatgaaaag gctatacatg atcgtttcga gcaacgcatc
     4681 gaagcaggac tgcagcagac gcggacaagt cgcgagaaac atcactatag tcggagcgcc
     4741 gtcgaatggc gag
//
LOCUS       DAOQUC010000104         4748 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_19958_length_4748_cov_2.914341, whole genome shotgun sequence.
ACCESSION   DAOQUC010000104 DAOQUC010000000
VERSION     DAOQUC010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4748)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4748)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4748
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19958_length_4748_cov_2.914341"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1375
                     /locus_tag="PKH07_02830"
     CDS             <1..1375
                     /locus_tag="PKH07_02830"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HNT33913.1"
                     /translation="FTVTNTPTNTPTNTPSNTPTNTPTNTPTNTPTNTPTNTPTATNT
                     PTNTPTNTPTNTPTRTPTSTNTPTNTPTNTFTNTPTNTPTATNTPTNTPTNTSTNTPT
                     PTPTSSGPNLIVDNAESGYSEVQVDSAWSAGTSATDKYGADYRYNTAGAGNDKATWQV
                     SISQAGWYDVFVWYPQGSNRANNSPFTVYHNTGSQTFNVNQTTNGGQWNYLGTFGFGA
                     TNYTVVLSDLAESGKIVMADAIRWSWSATQPTNTPTNTPTQYTTHEATIDTTQDTHIR
                     NYNSTYMGYNYGVYNRLLIGYYNGFGFYNQRALVQFNLSSIPSNATVTNCAISLYQDL
                     GSSHASSNQSHVVHIYRITSSWSEGTKNGAAGSVCWNDQPTKNNTSLASLTINGVAAG
                     TWRDFSGTSLKNVVQGWINGSYANYGVMVMNDVSNENDDGAHKFYSDDMTSGKPKIWI
                     QYTVPVP"
     gene            1601..4060
                     /locus_tag="PKH07_02835"
     CDS             1601..4060
                     /locus_tag="PKH07_02835"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HNT33914.1"
                     /translation="MKKILMKNRFLVMALMLLIPVTSGWAESVGCIGGAAEPSVQAAK
                     VSAADLDKAFTQASKEFGVPVDILKAVAQVESNMVQAGPTCDYGYGVMHLVDNGYCQT
                     LQEAAALVGVDADVLKQDAAANIRAGAALIAKYTKETVGKAKEARDYFEALKKFSGLI
                     DEQLQESQAGEYFRVLGVDVADLKKATVAIQSDDYGPAIWNAADPSNYTVGRGGVAVD
                     RWVNHWIGVGTYAGAISWFKNPASNVSAHFVCRSSDGQLTQMVRFANKAWHCGYWNAR
                     SIGIENEATESNPGLWNSTAMLNASAVACRYVCNLYGFPKTRSYIVGHKEVPYASTSC
                     PGPLPWTTYMNIVNAVTNTPTPTPITYAAHWVAQSYTSTMTSGTTATCWVEYMNDGTA
                     VWGHAATRLGTTQPRDRSSVFYTAGNWIGANRPTEVDQTSVSKGQVGRFTFIMTAPNV
                     GTTTTYTEYWGLVEEGVTWFGPPDNTVWFQITVNPSGATATPTNTPTATNTFTNTPTR
                     TPTPTFTFTNTATNTPTRTPTNTPTRTPTATPAVPVEIILDDGDTGYSKSGSWTTTTG
                     GGTEPINGDYDWLSTVTGSETGWAKWQPATLLGCYYDVYVRYKQGTNRATDAPYTVDY
                     DGGSTIVDVNQSINGGTWVYLGRFPMSTGKSVKLSNGPAVASKVVIADAVKFTPCAAT
                     TVTVDDGEAGYAKSGAWTTTTGGGTEPLNADYDWASTTTGAETASATWAPTLLKGGNY
                     QVYVRYRMGTNRASDAPYTVYYNGGSQTVDVNQQVNGGTWVLLGTYNFSYGSSGYVKL
                     SNGPAEASKVVIADAVQFVPQ"
     gene            4275..>4748
                     /locus_tag="PKH07_02840"
     CDS             4275..>4748
                     /locus_tag="PKH07_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HNT33915.1"
                     /translation="MKKISVLMGICCLIAMVTAQIASAQPASNSPQSAEQGTGTSQVP
                     ITEAKKGEASMATERPGYDDTPYIPGSKYRVHDKERPYPPAVDPGTASTQTVPGQPPS
                     DAVVLFDGTDLSKWTGKEGVAKWKVENGYMEVVPKTGDIETREHFGDCQLHLEWAA"
BASE COUNT         1272 a         1305 c         1227 g          944 t
ORIGIN      
        1 gttcacggtg accaacacac caaccaatac gccgacaaac acgccaagca atacaccgac
       61 caacacacca accaatacgc caacaaacac tccgacaaac acaccaacca atacgccaac
      121 cgcaaccaat acaccaacga acactcccac caacacacca accaatacac cgacaaggac
      181 accaacttcc acaaacaccc ccacaaacac ccccacaaat acgttcacaa atacaccgac
      241 caacaccccc acagcgacca atactccaac aaatacacca acgaacacct caaccaatac
      301 gccaacacca acacccacca gctcaggacc caatctgata gttgacaatg cagaatctgg
      361 gtattccgag gttcaggttg attcagcttg gtctgctgga acgtccgcca cggacaagta
      421 tggagcggat taccgctaca acactgctgg tgcaggcaac gacaaggcaa cctggcaggt
      481 gagcatttca caggcgggtt ggtacgatgt gtttgtctgg tatccgcagg gaagcaaccg
      541 agccaacaat tcgcccttca cggtctatca caacaccggt tctcagacgt tcaatgtgaa
      601 ccagaccacc aacggcggac agtggaacta tctggggacg ttcggatttg gcgctaccaa
      661 ttacacggtt gtgctgagtg atctggccga atcgggtaag atcgtcatgg cagacgcaat
      721 tcgatggtcg tggagtgcaa cacaaccaac gaacacgccg accaatacac caacacagta
      781 tacaacccat gaagcaacca ttgatacgac acaagacaca cacatccgca actacaacag
      841 cacctatatg ggttacaact atggcgttta caaccgcctg ctgattgggt actacaacgg
      901 ctttggattc tacaatcaga gagcattggt gcagttcaac ctgtcttcga ttccatcgaa
      961 tgcaacggtg accaactgcg ccatttccct gtatcaggat ctggggtcgt ctcatgccag
     1021 cagcaaccag agccatgtgg tgcatatcta tcgaatcacg agttcctggt cagagggcac
     1081 caagaatggc gccgctggtt ccgtctgttg gaacgaccaa cccaccaaga acaacacgag
     1141 tctggcttca ttgaccatca atggagtggc ggctggcacc tggcgtgact tctcaggaac
     1201 cagtctgaag aacgtggttc agggttggat taacggatcg tatgccaact atggtgtcat
     1261 ggtgatgaac gacgtcagta acgagaacga tgatggcgct cacaagttct actcggatga
     1321 tatgacatca gggaaaccaa agatctggat tcagtacacc gttccagttc cttaggtcgc
     1381 agttcttgtt tcgagtcgag tcccgacgat catgtcggga ctcgactctt gaagtctgac
     1441 gcggaggaat gaaccgaaat ggagtcctgt atcgaacggt gaaaggaggg atgtttatcc
     1501 gaaatgttcc tgagaacttc tgatgtgtta tgaaaagggg aatcgtttgt tctgttttgg
     1561 ctcattcgga ttgtgagcag tccgaaccga ggaggaaaaa atgaagaaga tcttgatgaa
     1621 gaaccgattt ctggttatgg cgctgatgct gcttattcca gtgacatcag gctgggcgga
     1681 atcggttggt tgtatcggtg gtgcggcgga accctctgtt caggcagcca aagtcagcgc
     1741 ggctgatctg gataaggctt tcacgcaggc atccaaagag tttggtgttc ccgtggatat
     1801 cctgaaagcg gtagcacagg ttgaatccaa catggttcag gccggaccga cctgcgacta
     1861 cggctacggc gtaatgcatc tggtagacaa tggctactgc cagacgttac aggaagccgc
     1921 tgcgcttgtt ggagttgacg ccgacgtcct caaacaagat gctgctgcaa acatccgtgc
     1981 aggtgctgcc ttgatcgcca agtacaccaa ggaaacggtg ggcaaggcaa aggaagcacg
     2041 ggactatttt gaagcactca agaagttcag tgggctcatt gatgagcaac tgcaggaatc
     2101 gcaggcaggc gagtatttcc gagttctggg tgtggacgtt gctgatctga agaaagcaac
     2161 agtcgcgatc cagtctgacg actacggacc cgctatctgg aacgcggcgg atccctccaa
     2221 ctacacggtt ggccgaggtg gtgttgccgt tgaccgttgg gtgaaccact ggattggcgt
     2281 tggaacttat gcgggagcca tttcctggtt caagaatccg gcctcaaatg tgagtgcaca
     2341 ctttgtgtgt cgctcttccg acggccagtt gacccagatg gttcgctttg ccaacaaggc
     2401 ttggcattgc ggatactgga atgcacgaag catcggcatc gagaatgaag caaccgagtc
     2461 aaacccagga ctctggaaca gcacggcaat gttgaatgct tcggcagtag cgtgcagata
     2521 cgtgtgcaat ctttatggtt ttccgaaaac ccggtcatac attgtcggtc acaaggaagt
     2581 gccctacgca tcaacttcat gcccaggccc acttccatgg acaacctaca tgaatatcgt
     2641 caatgcggtg acgaacactc caacgccaac acccatcact tatgctgcac actgggtcgc
     2701 gcagtcgtac acctcaacaa tgacttcggg caccaccgca acctgctggg tggagtacat
     2761 gaacgatggc acagccgtct ggggtcatgc agcaacccga ttggggacaa ctcagccgag
     2821 agatcgttcc agtgtcttct atactgctgg caactggatc ggtgccaatc gtcccacgga
     2881 agttgaccag acgtcggtat ccaagggcca ggtcgggcgc ttcacgttta tcatgactgc
     2941 tccgaatgtc gggacaacaa cgacgtacac cgagtactgg ggcttggtgg aagagggagt
     3001 gacctggttt ggaccacccg acaacacagt ctggttccag atcaccgtca acccgtctgg
     3061 tgcgacagcc acaccgacca acactccaac ggcaacgaac acgttcacca atacaccaac
     3121 aagaacgccc accccgacgt tcactttcac aaacacagca acgaataccc cgacccgaac
     3181 accgacgaac accccaaccc gaaccccaac ggcaacaccc gcagtgcctg tcgaaatcat
     3241 ccttgatgac ggagataccg gctacagcaa gtcgggaagt tggacgacaa cgaccggtgg
     3301 tggcaccgag cctatcaatg gtgactatga ttggctgagc acagtgactg gttcggaaac
     3361 cggctgggca aagtggcagc cagcaactct gctgggctgt tactacgacg tctacgttcg
     3421 ctataagcag ggtaccaacc gagcgaccga tgctccttac accgtagact acgacggggg
     3481 ctccacaata gtggatgtca accagtccat caatggcggc acctgggtct atttgggaag
     3541 gttcccaatg tccaccggca agtcggtgaa actctccaac ggtcctgcag tggcaagcaa
     3601 agtcgtcatt gcagatgcgg tgaaattcac accatgcgcg gcaacgacgg tcacagttga
     3661 cgacggtgag gccggctatg cgaaatccgg tgcgtggaca accacgactg gcggtggcac
     3721 cgagcctctc aacgccgact atgactgggc aagcaccaca acaggtgctg agactgcatc
     3781 ggcaacatgg gcacccacgc tgctgaaagg cggcaactat caggtctatg ttcgctatcg
     3841 tatgggaacc aacagggcga gtgatgcccc ctataccgtc tactacaacg gcggttctca
     3901 gacggtggat gtcaaccagc aggtcaatgg tggaacttgg gtcttgctcg gaacctacaa
     3961 cttcagttac gggagttcag gatatgtgaa actctccaat ggtcccgcag aagcgagcaa
     4021 agtggtcatc gctgatgcgg ttcagtttgt tcctcagtaa gatcgagact gtgcgagtga
     4081 acgactcgaa gtaaatctga gtcattcacc atgtcacaac ccaagggctg gtgcctcaga
     4141 gtgccagccc ttgatcttta tgtcgaggcg gagtcatctg ttttgttcac cgcgattgac
     4201 gatgcaagac gcaaagtgta tctttctctc tcagtattcc gaacgtccta tccggtgaag
     4261 aggaaagaca gaccatgaaa aagatttccg tgttgatggg catctgctgt ctgatcgcca
     4321 tggtgacagc gcaaatcgcc agcgcgcaac ctgcatccaa cagtccacaa agcgcagagc
     4381 aagggactgg cacaagccaa gttcccataa ctgaagcaaa aaaaggagaa gcgtctatgg
     4441 ccaccgagag acctggttat gatgacaccc cgtacattcc tggctccaag taccgggttc
     4501 acgacaagga gcgtccctat ccaccggcag tggatcctgg cacagccagc acacagaccg
     4561 ttccaggaca gccaccgtct gatgcggtcg tcctgttcga cggaaccgat ctgtccaagt
     4621 ggacaggaaa agaaggggtt gccaaatgga aggtcgaaaa cggctacatg gaagtcgttc
     4681 ccaagaccgg agacattgag acacgtgagc actttggtga ttgccaactt cacctggaat
     4741 gggcagca
//
LOCUS       DAOQUC010000105         4734 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_20037_length_4734_cov_3.585168, whole genome shotgun sequence.
ACCESSION   DAOQUC010000105 DAOQUC010000000
VERSION     DAOQUC010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4734)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4734)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4734
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20037_length_4734_cov_3.585168"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="PKH07_02845"
     CDS             <1..183
                     /locus_tag="PKH07_02845"
                     /inference="COORDINATES: protein motif:HMM:NF018534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33916.1"
                     /translation="IGLQAEVSAPTENVTWYVNGVQYAKGGPPYQARWRLERGRHRIA
                     AVAGDSFGDEILVTVE"
     gene            355..558
                     /locus_tag="PKH07_02850"
     CDS             355..558
                     /locus_tag="PKH07_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019952608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33917.1"
                     /translation="MLRSIEATVETNGEVRLREPIRIKYPCRAIVTIIDEPVVSESAL
                     LSQESLALDWERTEEDEAWSHLQ"
     gene            540..878
                     /locus_tag="PKH07_02855"
     CDS             540..878
                     /locus_tag="PKH07_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006890590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HNT33918.1"
                     /translation="MVSPSVGSVVLVRFPFSDLSSSKLRPAVVLAAVDRDDWILCQIT
                     SNPYSDHHAVEIADSDFVSGGLMRTSYVRPGKIFSANTTVMYRSVGILKPMKLESIVD
                     AVLTILRPGP"
     gene            complement(1006..1746)
                     /locus_tag="PKH07_02860"
     CDS             complement(1006..1746)
                     /locus_tag="PKH07_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNT33919.1"
                     /translation="MNERRDSGHSTDAPTFLLSVVMPVYNEKDTILEIISKVQAVNVP
                     KEIVIVDDCSKDGTREILQGLSGQDNVRILYHEVNQGKGAAVWTGIQNAQGDIVIIQD
                     ADLEYSPEEYPRLLKPILNGHADVVYGSRFLGDERRVLYYWHTVGNRGLTFLSNVFTN
                     LNLTDMETCYKVFRTSVLRQLTIESKRFGIEPEITAKIAKLRCRIYEVPITYHGRDYS
                     QGKKITWKDALVAVWCIIKFNLLRRRVK"
     gene            complement(1743..2720)
                     /locus_tag="PKH07_02865"
     CDS             complement(1743..2720)
                     /locus_tag="PKH07_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNT33920.1"
                     /translation="MRVLVTGGAGFIGSYLVETLMARGDEVYILDDLSTGRFSNVERF
                     ENDPRFHCVVDDVAHEPVVDQLVQKVDVIYHLAAAVGVQLIVDEPVRTIETNIRGTEV
                     ILKHACRYRKHVLITSTSEVYGKSTQPKFKEDDDRIMGPTTKCRWAYAASKAIDEFLA
                     LAYWYEKRLPVVIVRLFNTVGPRQTGRYGMVVPRFVRQALQNEPLTIFGDGKQTRCFT
                     YVGDVVPALIQLLECKRAQGEIFNIGSDEEVSIEEMARRVIKKTGSAGGLQYISYEKA
                     YGEGFEDMQRRVPDTSKARELIGFAPKFNLDQILQAVVDYERKALGLEK"
     gene            complement(2723..3313)
                     /locus_tag="PKH07_02870"
     CDS             complement(2723..3313)
                     /locus_tag="PKH07_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015259450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HNT33921.1"
                     /translation="MSMESLEQDAVAFLRSNAEAQSRIANESVDLLLSVADTLVACIQ
                     AGGKILIFGNGGSAADAQHFAGEIVGRFLRERGPLAAIALTADTSVLTAIGNDYGFEE
                     IFSRQVQGLGKPGDVAFGISTSGNSSNVLKALQVARDGGMKTIALTGPLPNRAAETAD
                     QVLCVDSKQTPIIQQIHITFIHLLCLLVEKAIFGEE"
     gene            3493..3684
                     /locus_tag="PKH07_02875"
     CDS             3493..3684
                     /locus_tag="PKH07_02875"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNT33922.1"
                     /translation="MREMLISWEEIAGYLRISRKTAQRMEKSSELPVRRKGRGFVYAF
                     KDEVDTWLNRPPQQLSNTE"
     gene            complement(3835..>4734)
                     /locus_tag="PKH07_02880"
     CDS             complement(3835..>4734)
                     /locus_tag="PKH07_02880"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNT33923.1"
                     /translation="YVPLYLSNYCSGGCSYCGFASDREVPRSRLERPEMIEEMQALKA
                     QGFEDVLLLTGECCPEADFEYLLDATALAAEHFHNVSVEAFAMTQREYGRLGDAGCTG
                     ITLYQETYDTRLYEKLHRWGEKKDFRYRLEAPTRALEAGLRWAGIGALLGLSNPVSDL
                     IALFAHAQYLMKRFWRSGVLISFPRICEQRGDYIPEFAVDEQLLAQAIFAFRICLPDV
                     PLVLSTREGQRFRDGIAGVGISRMSIASRTTVGGYAHSAADPKDGQFLITDTRDVETF
                     CAALQAKRLEPVFKNWDRVFQNL"
BASE COUNT         1151 a         1311 c         1182 g         1090 t
ORIGIN      
        1 atcggacttc aggctgaagt gtcggctcca accgagaacg tcacttggta cgtgaacgga
       61 gtgcagtacg cgaaaggtgg ccctccctac caggctcgat ggcgtcttga acgagggaga
      121 cacagaattg ctgctgtcgc gggagactct ttcggcgatg aaatcctggt gactgtggaa
      181 taggcagcgc tcctagagtc tatgcgatca gattggtttc acgtcgagat ttgccgaaac
      241 atcccttgtt gggtatttct gattcagttg cagaagaaaa ccaggcgggc tactatacga
      301 aggatgaacg acacttgaaa atgtgtgcag caatgccatt tctgaggtga cgagatgttg
      361 aggtcaatag aggcaacagt tgagaccaac ggagaagttc gcttgcgtga accaattcgt
      421 atcaaatacc catgtcgagc cattgtaacg atcattgacg agcccgttgt ctcggaatca
      481 gccttgctga gccaagagtc actggcattg gactgggagc gtactgagga ggacgaagcg
      541 tggtctcacc ttcagtaggc tctgttgtgc ttgtcagatt ccccttttca gatctctcca
      601 gcagcaaact ccgtccagct gtcgtgctcg ccgctgtaga tcgggatgac tggattctct
      661 gtcagatcac aagcaatccc tattctgacc accacgctgt tgaaatcgct gattctgact
      721 ttgtgtcagg aggtcttatg cgaaccagtt acgtacggcc aggcaagata ttctctgcca
      781 acacgactgt catgtaccgg tctgtcggca ttctcaagcc gatgaaactc gaatccatag
      841 ttgacgcagt tctcacaatt cttcgtccgg gaccatagac aatacgttcc cttgtcatcc
      901 atgctcccgg agacacagag tcccagaggg gcgtgtcctt tattgagcaa gcgttcgaac
      961 gagcgggcac agcccacgca ccatcatgag ctggcctgcg ctcttctact tgaccctgcg
     1021 gcgtaagagg ttgaacttga tgatgcacca gacggcaacg agcgcatctt tccaggtaat
     1081 cttcttgcct tgggagtaat cgcgtccatg gtaagtgatg gggacttcat agattcggca
     1141 ccgcagcttc gcaatcttgg cggtgatttc cggctcgatc ccgaaccgtt ttgactcgat
     1201 agtcagctgc cggagaacgg aagtccggaa gaccttgtag caggtttcca tgtcagtcag
     1261 attcagattg gtgaacacat tggacagaaa cgtcagtcct ctgtttccca ccgtatgcca
     1321 atagtacagc acacgtctct catcgcccaa aaagcgggaa ccgtaaacga cgtctgcatg
     1381 tccgttgaga atcggtttca gaagtctggg gtattcttcg gggctgtatt caagatccgc
     1441 atcctggatg ataacaatat ccccttgggc gttctgaata ccagtccaaa ccgcagcgcc
     1501 cttgccctgg ttcacctcat ggtagagaat tcgaacattg tcctgaccgc tcagtccctg
     1561 aagtatttct cgtgttccgt ccttggaaca gtcgtctaca atgacgatct ccttgggcac
     1621 attgaccgcc tgaactttgc tgataatttc caggatggta tctttctcat tgtagacggg
     1681 cataacaacc gacagaagaa acgtcggtgc atcggttgaa tgtccggagt ctcgtctttc
     1741 gttcatttct ccagtccaag tgcctttcgc tcatagtcca caacggcctg cagaatctga
     1801 tcgaggttga atttcggggc aaatccaatc aactcccgcg ccttgctggt gtcgggaacg
     1861 cgccgctgca tgtcttcaaa tccctctcca taggctttct cgtaggaaat gtactgaagg
     1921 cctccggcac tgcctgtctt cttgataacc cgacgagcca tctcttcgat ggagacttcc
     1981 tcatcactgc cgatgttgaa aatctcacct tgcgcacgtt tgcattccaa gagttggatc
     2041 aacgcgggaa cgacgtcccc gacataggtg aagcagcgcg tctgcttgcc atccccgaaa
     2101 atggtcaacg gctcgttctg aagcgcctgt cggacaaatc tcggcacgac catgccatag
     2161 cgaccggtct gtctcggtcc gacagtgttg aaaagtcgga caatcaccac gggcagacgc
     2221 ttttcgtacc aatacgccaa cgccagaaac tcatcaatgg ccttcgacgc tgcataggcc
     2281 caacggcatt tggttgtcgg acccatgatc cgatcatcgt cttctttgaa tttgggttgt
     2341 gtgctcttgc cgtagacttc ggaagttgat gtgatcagaa catgcttccg atatcggcag
     2401 gcgtgtttca aaatgacttc cgtgccgcgg atgttggtct caattgtgcg aacgggttca
     2461 tccacgatca actgcactcc caccgccgcc gccaagtggt agatcacatc caccttctgc
     2521 accagttgat ccacaacggg ttcatgcgcg acatcgtcca caacgcagtg aaaccgcgga
     2581 tcattctcaa aacgctcaac gttcgaaaaa cgaccggtgg aaaggtcgtc gaggatgtag
     2641 acctcatcgc cgcgagccat caatgtttct accagatagg agccgataaa gcccgcacca
     2701 cctgtcacca atactctcat gattactcct cgccaaaaat ggctttttcg accaacagac
     2761 acaggagatg gatgaacgta atgtggatct gttggataat cggtgtctgc ttgctgtcaa
     2821 cgcacaggac ctgatcggcc gtctccgccg cccgattcgg aagaggtccc gtcaacgcga
     2881 tcgtcttcat cccgccgtct cgggcaacct gaagagcctt cagaacgttt gaagaattcc
     2941 cactggttga aattccaaac gcaacatctc ccggcttgcc caaaccctga acctgcctgg
     3001 aaaaaatctc ctcgaatcca tagtcgttcc cgattgcggt cagcaccgag gtgtcagccg
     3061 tcagggcaat cgccgccaac ggaccgcgtt ctcgaagaaa tctccccaca atttcgcccg
     3121 caaaatgctg tgcatccgcc gcactgccac cattgccaaa aatgagaatc ttgccgcctg
     3181 cttggataca cgcaaccaga gtgtctgcga ccgacagcag cagatcgact gactcattgg
     3241 cgattctgct ctgcgcttcg gcattgctcc gaaggaaagc gacagcatcc tgttccaggg
     3301 attccatgga cattacattt tcaccatttc aaccgtctgg actcattgcc cgtccgttgc
     3361 tcgagttgat gcgtaaaggt aggcgaagac caacaatgct gtcaagaaag atggaaataa
     3421 tcttgacaaa atcaactgaa catttgtaca ctaccaccga ataaaatgaa tataacaaat
     3481 ggaggtggaa ccatgagaga gatgttgatc agttgggaag aaatcgccgg ataccttcgc
     3541 atttcccgaa agactgccca gagaatggag aaatcgtctg aattgcccgt tcgcagaaag
     3601 ggacgcggct ttgtctatgc attcaaagat gaagtggaca catggttgaa tagaccacct
     3661 caacaactca gcaataccga ataaaacgaa taccactatg agtggtacgc tgccttggat
     3721 ctgtcacagc gtgacggctt ttgatgctca gcggcaggcc tcccctgttt tctggagaga
     3781 tagaggagca tccttacgca tcgggcgaag cccatccgac acgtccatgt gccactatag
     3841 gttttgaaac acccgatccc agttcttgaa gaccggttcg agtcttttcg cttgaagtgc
     3901 ggcgcagaaa gtctccacgt cgcgtgtgtc ggtgatcaga aactgaccat cctttggatc
     3961 agcagccgaa tgggcgtagc caccaacggt ggttcggctt gcaatgctca tgcgagaaat
     4021 ccccactccg gcgattccat ccctgaaccg ttgcccttca cgtgttgaga gaaccagagg
     4081 aacgtcggga agacagatcc gaaaggcaaa aatcgcctga gcaagcaact gttcgtccac
     4141 cgcaaactcg ggaatatagt ctccacgctg ctcgcagatg cgcggaaagg agatgagaac
     4201 cccagagcgc cagaaacgtt tcatcagata ttgcgcatgg gcaaagagag caatcaaatc
     4261 ggaaacggga ttcgacagtc cgaggagcgc accgataccc gcccaacgca gccccgcttc
     4321 caaggcacga gtcggcgctt ccaacctgta gcggaaatcc ttcttctctc cccaccggtg
     4381 cagcttctcg tagagcctag tgtcgtaggt ttcctgatag agcgtgatgc ccgtacatcc
     4441 ggcatcaccc aggcggccgt attccctctg ggtcatagca aacgcctcga cggacacgtt
     4501 atggaaatgc tccgctgcca gagccgttgc atcgagcagg tattcgaagt ccgcttccgg
     4561 acagcactct ccagtgagca aaagtacgtc ttcgaacccc tgcgccttga gagcctgcat
     4621 ctcttcgatc atttccggac gttccagccg ggagcgaggc acttcgcgat cggaggcaaa
     4681 tccgcagtat gagcatcccc ccgaacagta attggaaagg tacaacggta cata
//
LOCUS       DAOQUC010000106         4715 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_20158_length_4715_cov_3.096352, whole genome shotgun sequence.
ACCESSION   DAOQUC010000106 DAOQUC010000000
VERSION     DAOQUC010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4715
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20158_length_4715_cov_3.096352"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3067
                     /locus_tag="PKH07_02885"
     CDS             <1..3067
                     /locus_tag="PKH07_02885"
                     /inference="COORDINATES: protein motif:HMM:NF012906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HNT33924.1"
                     /translation="VGESEDGFTPFEVEIGNVLRRDGENELLVGCAGIEALSENGRKA
                     CPPGSFWGHHVVGLWQDVTLIRRPETFIKNTFVCTEIEPASLEVSVCLDSAAPESKQL
                     RLVHELFPWRPDDQNQSPRFVAADFVKAVPDAIQTHRTTTVVKEANLWSPEHPNLYIL
                     RSRLINSQEGLVDENTVRFGFREFRVEGDMFLLNGAPRRLRGDSWHYLGTLQQSAQYA
                     RLWYRLARETGLNVVRLHAQPYPTSYLDVADEEGMLIISESALWASGLLMSYSQDFWR
                     RYESHLKRLVVRDRNHPSVVLWSIANETFAAHDIDPNDGASDREEIASHLSEAMDSLR
                     ILDPTRITYSDGDQDLNGRTSVHGLHYPHILNRPEIRKPVVIGEEGLMHYADPELVAS
                     NAGDEVYASREAWLEGIANYITPFFHGYRQWAALIAPFNTVWYALDPLPFEDRPNPSI
                     RQTHSQKRLERIPKFSLTLNPSFDNSLPQWRPNRLYNTIKDLMTPVRFYVDRVTRFYA
                     QQETGLMVVIQNDSSESQDLTLECYFNRELTPAPIFKFGCVLEPTEKRALMMPFAAPA
                     SSEIVSATLSFFLSNSGKELFAEDRGIAIFPALDRWPTLIEPEARILYMGKREMASVL
                     PRHVHAERIADASLEGIDILILGPEAIPNYDEVQQIKYRVEHGLRLLAMEPKKILLDA
                     FDVTATPVFSTYSKVSILAPDHPIFEGLSGEFLDHWRPDGVVGTWGFSDCAGRDARIL
                     AVSTTGEDVLMEIRQGRGSVIFCGCDLSTCLREEPAACLLLYNILKYLSHFTETRRYP
                     VLAWTEAESQLRSLLLAVGVEDSSFLQSLPSEVSGSVATLIVDASAMKPNEEQLDWIS
                     TFADSGGQVLISNLRPETLSYYGRILPAALRISECTAEQLIRSQAHSLLNGISNANLY
                     WIEKESLRPIVHYSISAANIQEPAVALLKVPRIDWRAWCWQHEAVKTSALLQSTLRAE
                     PDQVGLLAYSYGEGSIVICQLIPDNDYAKCRKVFGSLLTNMGSTFRLAR"
     gene            3216..4679
                     /locus_tag="PKH07_02890"
     CDS             3216..4679
                     /locus_tag="PKH07_02890"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HNT33925.1"
                     /translation="MGRRHVSRRLFLRRVGEGFAAAATTRLAMCDTRSSADQPNVLFI
                     CIDDLNDWVGCLGGHPDVKTPNLDRLASRGVLFTQAYCASPVCNSSRASLMTGIRPST
                     SGVYMNTQPWRSNSLLRDVATLPQHFMAGGYTALGAGKIYQDSFPDPPSWEEYWPSQL
                     INRPDDPMPPVRPLNGIPYTGEFDWGPIDVEDEEMGDWQVTDWVIEQLRRDFRRPFFL
                     ACGIYRPHLPWYVPRKYFDLYPIDKVTLPEVCEEDLDDIPPAGRSFVGGQDHANVLRY
                     DQWRFAVQAYLASISFADTCVGRLLDALDQSPYRDGTFIILWSDQGWHLGEKLHWRKF
                     ALWEEATHNVLMMAGPGIVPGGRCKAPVSLLDIYPTVTDLCGLSSPKYLEGRNLKDLL
                     KNPGVQWQWPVVTTHRKGNHSVRSERWRYTRYADGSEELYDHTEDPMEWTNLASRSDL
                     SDIKSDLARWVPEYDAPYDPVPERGLPPEIGKVGILE"
BASE COUNT         1091 a         1246 c         1236 g         1142 t
ORIGIN      
        1 cgtgggtgag agcgaagatg gcttcacccc ttttgaggtg gagatcggaa atgtcctccg
       61 gcgagatggg gagaatgagt tgctcgtcgg ctgtgcgggc attgaagcat tgagcgaaaa
      121 cggtcgaaag gcttgtccac caggttcctt ctggggacat catgttgttg gtctctggca
      181 ggacgtcaca ctcatccgtc ggcctgagac ctttatcaag aacacctttg tctgcacaga
      241 gatagagcct gcgtcgcttg aggtttcagt ttgcctggac tcagcagccc ccgagtccaa
      301 acagcttcgc ctcgtgcatg agctgtttcc ctggcgaccc gatgaccaga accaaagccc
      361 caggttcgtt gctgccgact tcgttaaagc cgttccggat gcaattcaga cacacagaac
      421 caccactgtc gtaaaggagg caaatctctg gtctccggag catcctaacc tctacatctt
      481 gagatcgcgc ctcatcaatt ctcaggaagg gcttgttgat gagaatactg tgcgctttgg
      541 atttcgtgaa ttcagagttg aaggagatat gttcttgctc aatggagcgc ctcgcaggct
      601 ccggggtgac tcgtggcact atctgggaac tctgcagcag tctgcacaat atgcacgtct
      661 ctggtatcgg ttggcgcgcg agacaggcct caatgtcgtt cggctccacg cgcaacccta
      721 tccgacatcc tatctcgatg ttgccgacga agagggaatg ctgatcatca gcgaaagtgc
      781 tctctgggct tccggactcc tcatgagcta cagtcaagac ttctggagaa gatacgaatc
      841 tcatctgaaa agacttgttg ttcgtgatag aaatcatccg tcggttgttc tttggagcat
      901 tgccaacgaa acctttgcag cacacgacat tgatcccaac gatggagcct ctgaccgcga
      961 agagattgcc tctcatcttt ccgaggcaat ggactctctc agaatcctgg atccaacccg
     1021 aatcacctat tcagatgggg atcaggattt gaatggtcgt acctcagtcc acggtcttca
     1081 ttatccgcat attctcaaca gacccgagat tcggaaaccc gtcgtcattg gagaggaagg
     1141 gttgatgcac tatgcagatc cagagttggt ggcgtccaat gctggtgacg aggtgtacgc
     1201 gagcagagag gcttggcttg aaggcattgc caactacatc acgccctttt tccatggata
     1261 cagacagtgg gctgctctga ttgcaccttt caacacggtg tggtacgccc tggatccctt
     1321 gccgtttgaa gacagaccca acccgtccat cagacagaca cactctcaga aacgtctgga
     1381 gcggatcccg aagttcagcc tgactttgaa tccgagtttc gacaattccc tcccccaatg
     1441 gagacccaac cgcctctaca ataccatcaa ggatttgatg actcccgttc gattctacgt
     1501 tgatcgcgtt acaagattct acgctcagca agagaccggt ttgatggttg tgattcagaa
     1561 tgattcttct gaatcgcaag atctgacact cgaatgttac ttcaacagag aactcactcc
     1621 ggcacctatc ttcaagttcg gctgtgtgct cgaacccacc gaaaaaaggg cacttatgat
     1681 gccctttgcg gcaccagcct caagtgagat tgtctcagca acattgagtt tctttctgtc
     1741 taattccgga aaggaacttt ttgctgagga taggggcatt gctatctttc ctgctctgga
     1801 cagatggcca acactcatcg agcctgaagc acgcatcctg tatatgggaa agcgtgaaat
     1861 ggcctctgtc cttccaaggc atgttcacgc cgagagaatt gcagacgcat ctctggaagg
     1921 tattgacata ctgattcttg gccctgaagc aattcccaac tatgacgaag ttcaacagat
     1981 caaataccgt gttgaacacg gtttgagact gctcgcaatg gagcccaaga agattctcct
     2041 cgatgcgttt gacgtaaccg caacacctgt cttcagcacc tattcgaaag tctcgattct
     2101 tgcacctgac catccgatat tcgaggggct ctctggggag tttctcgatc actggcgtcc
     2161 ggatggagtc gtgggcactt ggggtttctc agactgtgct ggacgagatg caaggattct
     2221 tgctgtttcc accaccggcg aggatgtcct catggaaatt cgccagggaa ggggcagcgt
     2281 gattttctgt ggctgcgact tgagcacgtg cctcagggaa gaacctgcgg cgtgtctcct
     2341 tctctacaac attctgaagt acctttctca tttcacggag actcggcgat atccagtgct
     2401 ggcgtggacg gaggctgaga gccaacttcg aagtctgctt cttgccgttg gagtcgagga
     2461 ttcatcattc ctgcaatctc ttccatccga agtgtctggg tcagtggcaa ccttgattgt
     2521 tgacgcttcg gcgatgaaac cgaatgagga gcaactcgac tggatctcga catttgctga
     2581 ttccggtggt caagtcttga tctcaaacct gcgtccggaa acactctcgt actatggccg
     2641 cattcttccc gcagcgctga ggatatccga atgcaccgcc gagcaactca tcaggagcca
     2701 ggcgcactcc ctgctgaacg gcatttcaaa tgcaaacctc tactggatcg agaaggaaag
     2761 cctcagacca atagtgcact acagcatttc ggcggcgaac attcaggaac cggccgttgc
     2821 gcttctgaaa gttcctcgga tcgactggag agcctggtgc tggcagcatg aagccgtcaa
     2881 gacatctgct ttgcttcagt caactctccg cgctgaaccg gatcaagtcg gcctgctcgc
     2941 ctattcatac ggggagggat caattgtcat ctgccaattg atccccgaca acgactatgc
     3001 taagtgtcgg aaagtgtttg gaagtctctt gaccaatatg ggctcaacgt ttaggcttgc
     3061 tcgttgactg agagaccatt tggacttcac gtttcgcaag gactcttcca ggcgggccgt
     3121 cttgggatca aaatcgccac aatgggcagt attcgggcgg caggggtgag acaccctaac
     3181 tgattttcca ggtttgggtt tggaaggaaa catacatggg caggcggcac gtatcgagac
     3241 gtctcttcct caggcgggtt ggagagggat ttgcagcggc tgcaaccacg cgacttgcca
     3301 tgtgtgacac gcggtcttct gccgaccagc cgaatgtact cttcatctgt attgatgacc
     3361 tgaacgattg ggtggggtgt cttggggggc acccggatgt caagacacca aatctcgaca
     3421 gacttgcttc ccgtggtgtc ttgtttaccc aggcttattg tgcctctccc gtatgcaact
     3481 cctcaagagc gtcgcttatg acgggcatac gtccgtctac gtcgggtgta tatatgaaca
     3541 cgcaaccctg gcgctccaat tccctgcttc gagatgtcgc aacgcttcct cagcacttta
     3601 tggcaggcgg gtacacggcc ttgggagcag gaaaaatcta tcaggattca tttccggatc
     3661 ccccttcctg ggaagaatac tggccgtcac agctcatcaa taggccagac gacccaatgc
     3721 ctcccgttcg tcctttgaat ggcatccctt acacagggga gttcgactgg ggcccaattg
     3781 atgtcgagga cgaggagatg ggcgattggc aggtaacaga ctgggtaata gaacaactgc
     3841 gacgagactt caggagaccc ttctttttgg cctgtggcat ttaccgacct caccttccct
     3901 ggtacgtgcc gcgcaagtac ttcgacctct atccaatcga taaagtaacc ctgcccgaag
     3961 tctgcgagga agacttggac gatattccgc ctgcaggacg gagttttgtc ggaggtcagg
     4021 accatgccaa tgttctcaga tacgaccaat ggcggtttgc tgtacaagcc tatctggcct
     4081 cgatctcctt tgcggatacc tgtgtcggta gactgctcga cgctctggat caaagtccct
     4141 atcgcgacgg aacattcata atactatggt cagatcaggg ctggcacctt ggtgagaagc
     4201 ttcactggcg caagttcgca ctttgggaag aggccacaca caacgtcctg atgatggcag
     4261 gaccagggat tgttccaggt ggcagatgca aggctccagt gagtcttctc gatatctatc
     4321 ccacagtcac agatctctgt ggcttgtcgt caccaaaata tcttgaggga aggaatctca
     4381 aggatctcct caagaatccc ggtgtgcaat ggcaatggcc tgtggtgaca acccatcgta
     4441 agggaaacca ctcggttcga tcagagcgtt ggcgctatac tcgttacgca gacggctcgg
     4501 aagaactcta tgaccacacc gaagacccta tggagtggac gaatcttgca agccgatcgg
     4561 acttgtcaga tatcaaaagt gatctggctc gctgggtacc ggaatatgat gccccatacg
     4621 accctgtacc cgagaggggc ttgcctccgg agattggaaa ggtcggcatc cttgaatgaa
     4681 tgactgtagc cacacaaaga tccaacagag gggcg
//
LOCUS       DAOQUC010000107         4696 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_20281_length_4696_cov_3.845938, whole genome shotgun sequence.
ACCESSION   DAOQUC010000107 DAOQUC010000000
VERSION     DAOQUC010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4696
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20281_length_4696_cov_3.845938"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(5..62)
                     /locus_tag="PKH07_02895"
     tRNA            complement(5..62)
                     /locus_tag="PKH07_02895"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(35..37),aa:Ala,seq:tgc)
     gene            438..1235
                     /locus_tag="PKH07_02900"
     CDS             438..1235
                     /locus_tag="PKH07_02900"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HNT33926.1"
                     /translation="MTDSNRWLELAVRAAREAGPILKRKFRTKIHKELKSEKNLVTEA
                     DKASEECLVKILREGCPDCTILTEESPEMKGASGMKWIIDPLDGTTNFAHGLATFSIS
                     IGLEMEGQIVVGAVYDPIHDDMYTACKGEGAFLNGCPIHVSETELLGDSLLVTGIPYS
                     VLTLEENNLDLFAAFCFEGQSVRRLGSAALDFCNVAAGFLDGFWELGLQPWDMAAGSL
                     IVQEAGGTVTDTTGAPIDIYAKRQVASNGRIHHQMLNVIRETQEDKD"
     gene            complement(1302..3779)
                     /locus_tag="PKH07_02905"
     CDS             complement(1302..3779)
                     /locus_tag="PKH07_02905"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HNT33927.1"
                     /translation="MYRRTKKEETLPRKTPVPKPAAPRPSKPKSGGNEDTGGCLRGCF
                     IVGLIFLFGFFGAGFGVLNVFLRGLPPLTALDKYRPQLVNRIYAGDGETKVAELFDEK
                     RDLVALEEIPQHLKNALLAIEDVRYFEHPGVDVIRIFGALVADIKMGRLAQGGSTITQ
                     QLARNLFQDRRKTIERKVKEALLALRIEKHYTKQEILEMYLNQVYLGRGTYGVKAAAE
                     FYYHKALTELTLSECASIVGLLKAPEEYSPVMNTEASRSRRNLVLRRMLDEKFITQEV
                     YDEAIAEPIAVQAGERTRAQQQVYHPYFVMYVQRLLQGVERPIGYSEPIVDPKEYKLG
                     GLTIETTLEPALQQAAEEAVRKGIVTLEQHRRKYRYGWGDPNYPYKSGQSLKTGEIYD
                     ARIKSVSGNQVRFDLPSVSTKQHEISLNLDEIWLDDFDVLKPEYYFRVKAATKEADRW
                     SFEYVWEPHVQAALVAIEPATGEVRAMVGGYDYFDKDPKQGGQFIRAIQALRQPGSGF
                     KPIIYATALNEGYSPATLLSDERITFNYGRQSWSPKNYHDRYYSLIPLSTALELSQNC
                     ATVRLLTAIGIGRVQEMAQRLGFVSPLSNDLSLSLGTSDVSPLEMAVAYGALANEGRR
                     VRPIFIRRITDRGGKVVYESRPYAFPALRPEIAFQVTNILKGVIRYGTGQKAGDIPYP
                     IAGKTGTTNNCMDAWFTGYSPHLAVAVYVGFDQKRSLGYQMTGSFCALPIWKAFMEKA
                     LPFCAEEIDPRNPPEPKSLDFREPSGMVWANICRFSGRLATPSCSSTEKHPFIAGTQP
                     TESCTSHGPGLSGEDFIGAESHDVEGL"
     gene            4200..>4696
                     /locus_tag="PKH07_02910"
     CDS             4200..>4696
                     /locus_tag="PKH07_02910"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HNT33928.1"
                     /translation="MNRESPSTVWTLPNLVTLARILLVPFFFGLTVYHYRALQTGENH
                     QTVYQAGAVVVFIFILISDGLDGYLARRNNRCTPLGSLLDPVADKLFVISSFALLVAF
                     KAIPIWLAVLVISKDVVLCLGWLLLQVLHYDSSIRPSRAGKATLLLLFLVVLESLLRS
                     PTIFR"
BASE COUNT         1052 a         1265 c         1250 g         1129 t
ORIGIN      
        1 acctgatcgg ggtcgaaccg actacctccg ccttgcaagg gcggcgctct cccagatgag
       61 ctacgtcccc aactgcttta ccgaaacgct acaataacac cccgtgggag aacgtcaagg
      121 actttcatca ttgcggagta tcagctcagt tacacagggt atggttgtca ttctgaatcc
      181 ttcggctgcg ctcaggacag gttccgcgac gctctgggag cggaatgaag aatctctctt
      241 tactgcagaa tccagagaga ttcctcactc cttcgccaag cctcagtcgt tcggaatgac
      301 agtctccgcc cttgtgtaac tgagccagta cgttgcggaa gcctttgggc ttaacattcc
      361 gaacgcaacc gtgtaagatt ctcagacttt gaaaacaggg gctgttcaat aacacacagc
      421 cttggaagga gtctttgatg actgattcaa accgctggct ggaacttgcg gttcgagcgg
      481 cgcgcgaggc cggtcccatt ctcaagagaa aattcaggac gaaaatccac aaggaactca
      541 agagtgaaaa gaaccttgtc accgaagcag acaaagcctc agaagagtgt cttgtcaaga
      601 ttctgagaga aggatgtccc gactgcacga tcctcaccga ggaaagccct gagatgaagg
      661 gtgcttcagg catgaagtgg atcattgatc ccctggatgg tacaacgaat tttgcccatg
      721 ggcttgccac tttctcgatc tccatcgggc ttgaaatgga agggcagatc gtggtcggtg
      781 cagtctacga tcccattcac gatgacatgt acaccgcctg caaaggggaa ggagcatttc
      841 tgaacggctg tccgatccat gtttcggaaa cggaactcct gggggattct cttctggtca
      901 ccggaattcc ttacagtgtc ttgactctgg aagaaaacaa tctggacttg tttgcggcgt
      961 tctgtttcga aggtcagtcg gtcagacggt tgggttctgc agcgttggat ttctgcaatg
     1021 tggccgcagg tttcctggac ggcttctggg aactggggct tcagccgtgg gacatggcgg
     1081 caggatcgct catcgttcag gaagcaggtg gaaccgtgac cgataccacc ggagcaccga
     1141 ttgatatcta tgcaaaacgt caggttgcgt ccaacggcag gattcaccac cagatgctca
     1201 atgtcatccg ggaaacacag gaagacaaag actgagaggg gaacgagaag tactctctca
     1261 cacgaactgt tttccgaaaa agactgcacc ctcgaagacg tttacagtcc ttcgacgtcg
     1321 tgcgattccg ctccgatgaa gtcctctccc gaaagacctg ggccgtgact cgtacacgac
     1381 tctgtcggct gggttccggc gatgaacgga tgcttctcgg tactggaaca agagggtgtg
     1441 gcaagtctcc cactgaatcg gcagatattt gcccacacca tgccactcgg ttctcggaaa
     1501 tcgagactct tcggctccgg cggattcctg ggatctatct cttcggcaca gaaaggaagc
     1561 gccttttcca tgaatgcttt ccagatcggc agcgcacaga atgatcccgt catctgatag
     1621 cccagagagc gcttctgatc gaatccgaca tagacggcca cggccaagtg cggagaatat
     1681 cccgtgaacc aggcgtccat gcagttgttc gtagtgcccg tcttgccggc gattggatag
     1741 gggatatcac ccgccttctg accggtgccg taacggatta cccctttcag gatgttggtg
     1801 acctgaaagg cgatctctgg ccttagagcc ggaaacgcat aggggcgact ttcatagacc
     1861 accttgccac cgcgatctgt gattcgtcga atgaaaatcg ggcgaactct ccggccttca
     1921 ttggcaagtg cgccgtatgc cactgccatc tccagcggac tgacatcgga cgttcccaga
     1981 ctcagactca gatcattgct caggggtgag acaaaaccaa ggcgctgtgc catctcctga
     2041 actctgccga tgccgatagc ggtcaacagc cttaccgtgg cacagttctg tgaaagttcc
     2101 aatgcggtgc tcagaggaat cagactgtag tatcggtcat ggtagttttt gggagaccaa
     2161 ctctgccttc cgtagttaaa cgtgattcgc tcatcactga gaagtgtggc tggagagtat
     2221 ccttcattga gcgcagtggc gtagatgata ggcttgaatc ccgatccagg ttgtcgcaat
     2281 gcctgaatgg cgcggatgaa ttgccccccc tgtttgggat ctttgtcgaa ataatcgtag
     2341 ccaccgacca tggcacggac ttcccctgtg gcgggctcta tggcaaccaa ggcggcttgc
     2401 acatgcggct cccagacgta ctcaaaagac caccggtcgg cttctttggt tgcagccttt
     2461 actcgaaagt agtattccgg cttgagcaca tcaaaatcgt ccagccagat ctcatcaaga
     2521 ttaagactga tctcatgctg ctttgtggac accgagggaa gatcaaagcg cacctgattg
     2581 ccactgaccg acttgattcg tgcgtcgtag atttcacctg ttttgagtga ttggccgctc
     2641 ttgtaaggat agttcggatc accccaaccg tatcggtatt tccgacgatg ctgctccaag
     2701 gtgacaatcc ccttgcgaac cgcttcttcg gctgcctgtt gcagagcagg ttccagtgtg
     2761 gtttcgatgg tcagaccacc gagtttgtac tccttggggt caacgatcgg ctccgagtac
     2821 ccgatcgggc gctcgacgcc ctgaagcaac cgttgaacat acatcacaaa ataggggtgg
     2881 tagacctgct gctgggcgcg tgtccgctcc cccgcctgta ccgcaatagg ttcagcgatt
     2941 gcttcgtcgt agacttcctg ggtaatgaac ttctcatcca gcatacgacg cagcacaaga
     3001 tttcttctgc ttcgagacgc ttcggtattc atcaccggtg agtattcttc tggagccttg
     3061 agcagtccga caatcgaggc gcattccgac agggtgagct ccgtcagagc cttgtggtag
     3121 tagaattcgg cggcagcctt gaccccgtat gttccgcgac caagatagac ctgattcagg
     3181 tacatctcca gaatttcctg ttttgtgtag tgcttctcga tgcgcagagc cagcagggct
     3241 tccttgacct tgcgttctat cgtcttgcgc cgatcttgaa acaggttgcg cgccagctgc
     3301 tgtgtgatgg tgcttccacc ctgtgcgagt cgtcccatct tgatatccgc aaccaatgcg
     3361 ccgaagattc ggatcacatc cacgccagga tgctcaaaat agcggacatc ctcgatggca
     3421 agcaacgcgt tcttaaggtg ctggggaatc tcttcgagtg cgaccagatc tctcttctca
     3481 tcgaacagtt cggccacctt ggtttcccca tcgccagcgt agattctatt gaccagttgc
     3541 ggacggtact tgtccagcgc cgtcaagggg ggaagccccc gcagaaatac gttgagcact
     3601 ccaaaaccgg caccaaaaaa accaaacaaa aagattaggc caacaatgaa gcacccacga
     3661 agacatccac cggtgtcttc attgccacca ctcttgggct tggaaggtct tggagcggca
     3721 ggcttgggaa caggtgtctt tcttggcagg gtctcttctt ttttcgttct ccgatacatg
     3781 agagctctct ctggatacgt tcgaacaatt gcagatcata cagcacaatc acacagactt
     3841 tcaaacggct tcggtattgg ctcggttctg tggagtggag gctgtcttcc agaacactgc
     3901 ggcttcgctc ctggcgacac tccggaaaaa gagtgtcctc tcccgagcaa gcctccaagg
     3961 agatgggcat agtccaagcg cccgcaggcg ctaacccgct cagcatgcag ttgacgaggt
     4021 caatgactct tgttaggttc ttgcgagtga atcaccgcgt cgtaacgcgg atgctcggaa
     4081 aagaaaccga ccccccatta cctctccttg gaaaggaggg aagttcgagg tcttgccctt
     4141 tccgataggg agttggagag agtcgcagtt tcccaggagc cgacggagga ctccgaacca
     4201 tgaatcgaga atccccttcg actgtctgga cgttgccgaa tctggtgacg ttggcgagaa
     4261 ttctgcttgt ccccttcttc tttggactca cagtgtatca ctatcgggct ttgcagacgg
     4321 gggagaatca tcaaacggtc taccaagcag gcgctgtggt ggtattcatt ttcatcctca
     4381 tcagtgatgg gcttgatgga tatcttgcgc gtcgcaacaa ccgatgcacc cctcttggct
     4441 ctctcttgga tccggtggcg gacaaacttt tcgtcatcag ttcatttgcg cttctggtgg
     4501 ccttcaaagc aattccgatt tggctggcag ttctggtcat cagcaaggat gtcgttttgt
     4561 gtctgggctg gctgctgctt caggttctgc actatgacag cagcattcga ccctcaagag
     4621 caggcaaggc gacattgctt ctcttgttcc tggtggtgtt ggaatcgctc ttgcgcagtc
     4681 cgacgatttt taggaa
//
LOCUS       DAOQUC010000108         4691 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_20321_length_4691_cov_3.518766, whole genome shotgun sequence.
ACCESSION   DAOQUC010000108 DAOQUC010000000
VERSION     DAOQUC010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4691)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4691)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4691
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20321_length_4691_cov_3.518766"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..852)
                     /locus_tag="PKH07_02915"
     CDS             complement(<1..852)
                     /locus_tag="PKH07_02915"
                     /inference="COORDINATES: protein motif:HMM:NF017009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33929.1"
                     /translation="MKNKKKSLKDVLYSDVSLSSLKDLLTTEINLPFGTQVQQSYDDA
                     FTAAGHSEEEMAKMVQELESQGRSVEDFLVEKGFLSKSDLIRAKAETQSTGLSFFQTL
                     VNMKIATPAALGDILTSLRREIIDRLEGVDLEGILLGKKLTTKDKLEATRDLAREKKI
                     SFEQALLETKAISFEKLSEVYQEKYSIPTAKLNLKTYDETLINLLPEDLMRSKNCVPF
                     GKDGDKVLVAMADPRDTRLIDKIQMSTNLDIVPHFADVRDIEKILAKYLAEPSVPAIA
                     SDLFSAES"
     gene            complement(883..1362)
                     /locus_tag="PKH07_02920"
     CDS             complement(883..1362)
                     /locus_tag="PKH07_02920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33930.1"
                     /translation="MKAHSQQRANQGFALIAVLATVLILTGVVTGLSKIAFQNMQFAF
                     NSMMLSKASFAAEAAVDAAAGMARANPDQRQISGEITPYQEGRETITGSYQLSRVRAS
                     RFGDSLKGIDPTREVLWVRASGEARRGENYRRLCRVRAILLPVQEDKFVRTLVWTEN"
     gene            complement(1441..2136)
                     /locus_tag="PKH07_02925"
     CDS             complement(1441..2136)
                     /locus_tag="PKH07_02925"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNT33931.1"
                     /translation="MRKQRLPLRARIHGMTLVEIIVAIVIAGVLGAMVFHMIISTGRM
                     NKAQTSELMRTMQARTRLERICADLERVPAPLVTNKVGLVGKNNKIGDVDADMLQLVK
                     AEFVGEGPDLRVQFSEVCYLVEKVIKDKKEVTVLKVRQDDQVDGTVTGEDRELSLDSK
                     TQQVHLNLRYRADQNLSDSAGWRDEWAVSDPLPRAIEVTLQIEDLASDSPKSKPRMIT
                     LTRVVMPKAAVSF"
     gene            complement(2158..2532)
                     /locus_tag="PKH07_02930"
     CDS             complement(2158..2532)
                     /locus_tag="PKH07_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33932.1"
                     /translation="MKNSKNGFAMVDVLLAVLVLAIGIGFLTGLFTTAKRNAGYAETL
                     TNASFVAEQAKNNLRLPRAKSPLNEEGLAKGTQTMGGLEYTWRASRIKSDPGTDRVVL
                     VVTWEDFMGLHNQRFLTSIPTR"
     gene            complement(2704..3399)
                     /locus_tag="PKH07_02935"
     CDS             complement(2704..3399)
                     /locus_tag="PKH07_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015756683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HNT33933.1"
                     /translation="MQLVRNIQSVMKRIGDAAERSNRDASEITLIAVSKTVTEAMIRE
                     AYACGLRIFGENRPQQLEGKASMLADLKDIQWHMIGHLQRNKVKSVLPVAALIHSVDS
                     LRLAEELNRHSIERGCETPILLEVNISGEQSKFGFAEQEALDACRFIATMQGIVVKGL
                     MTMAPFAADPDETRPVFTRVRQLADFIRDQAITRIEMSVLSMGMSNDFEIAIEEGATM
                     VRIGTALWEGLSE"
     gene            complement(3417..>4691)
                     /locus_tag="PKH07_02940"
     CDS             complement(3417..>4691)
                     /locus_tag="PKH07_02940"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HNT33934.1"
                     /translation="MRIRGIGYLWRIACASFCAWLGLIPIQAWHFGTLNALTVPANVA
                     AMPLVWLCTVGGGVLAVAGFLHSWISIPVAWIVRHLLISLGALVFWMADWPFAAMRMS
                     RPGWPLLVGYYGVIFIFFAKKEYVEITFSRLTLRKIHVVLVLVLVVVLLNGFFGGVST
                     LKVYFLPLGSGDCILIQAPGSGTILVDGGTPDKAFSSSRGIRLLTALNALRVKRIDCL
                     VLTHADSDHIGELKAVVESLPVRMFITNPVEDPTVIYAQLLQALQAHDILIRRVRAGD
                     RVEGFPGLNIEVVSPSQTVLSQPKVEDNDASLVIRASYGEFDLLLTGDITQKVERLLL
                     DELPVLESEVLKVPHHGSKYSSSSSFLRKVAPEVAVIQVGRNSYGHPHPDALARLEAV
                     CDLVMRTDLDGTVEIESNGTAYWVRDCSSASP"
BASE COUNT         1082 a         1330 c         1175 g         1104 t
ORIGIN      
        1 agactccgca gagaacaggt ctgaagcgat cgccggaacc gaaggttcag caaggtattt
       61 tgccagaatt ttctcaatgt cccgaacatc agcaaaatgg ggaacaatat ccagatttgt
      121 ggacatctgg atcttgtcta tcagccgtgt gtcgcgcgga tccgccatcg cgaccagaac
      181 cttgtcgccg tccttgccaa agggaacgca gttcttgctg cgcatcagat cttcgggaag
      241 cagattgatg agggtctcgt cataggtctt gagattcagc ttggccgtgg ggatggagta
      301 cttttcctga tagacctcgc tgagtttttc aaatgagatg gcctttgtct ccagaagcgc
      361 ctgctcgaaa ctgatcttct tctctcgcgc caaatctcgt gttgcttcga gtttgtcttt
      421 ggtggtcaat ttcttgccca gcagaatgcc ttccagatcc acaccttcca aacggtcaat
      481 gatctcacgc cgaagactgg tcagaatgtc acccagagct gccggcgtgg cgatcttcat
      541 gttcaccaac gtctgaaaaa aactgagccc tgttgactgg gtttcggctt ttgcgcgaat
      601 gagatcggat ttggagagaa agcccttttc aacaagaaag tcctcgacgc tgcgtccctg
      661 gctttccagt tcttgaacca tctttgccat ttcctcttcg ctgtggccgg cagcggtgaa
      721 ggcatcgtcg tagctctgct gaacctgggt gccgaagggc aggttgatct cggttgtcag
      781 gagatccttg aggctggaaa gactgacatc agagtaaagg acatctttca gtgatttctt
      841 cttgttcttc attgcattgc cacctgttca gcggtcaaga cactagttct ccgtccagac
      901 caaagtcctc acgaatttgt cttcctgcac gggcaagaga attgcgcgca cccggcagag
      961 tctccggtaa ttttcaccgc gtctggcttc accggaagct ctgacccaaa gcacttcgcg
     1021 tgttgggtct attcccttca gggaatctcc aaagcgggaa gccctgacac gggacaactg
     1081 ataggaacct gttatcgtct ctctgccttc ctggtagggc gtgatctctc cgctgatctg
     1141 acgctgatcc ggattggcac gcgccattcc ggctgctgcg tctaccgctg cttccgcggc
     1201 aaaagaggcc ttggaaagca tcatggagtt aaaggcgaat tgcatgtttt gaaaggcgat
     1261 tttggaaaga cctgtcacca cgccggtcaa aatgagaacg gtggcgagaa ccgcgatcag
     1321 ggcgaaccct tgatttgctc tctgttggct gtgtgctttc atgttggctt cctcgaaaat
     1381 cgtttgagcg tttctacctt gacccttgtc actgttacca caaaagcccc gaattccgtc
     1441 ttagaacgag accgctgctt tgggcatgac cactcgggtc agcgtgatca tcctgggttt
     1501 gctcttcggg ctgtccgaag ccaggtcctc gatttgcagc gtgacttcga tcgctctcgg
     1561 aagaggatcg ctgactgccc attcatcacg ccatcccgcg gaatcgctca ggttctgatc
     1621 tgcacgatag cgcaggttca gatgcacctg ctgggtcttt gagtccagcg agagttccct
     1681 gtcctcgccg gtcaccgtgc cgtccacctg atcgtcctgg cgaactttca agactgtgac
     1741 ttccttcttg tctttgatca ccttttccac caggtagcag acttcggaga actgcactcg
     1801 cagatcaggg ccttctccca caaactcggc cttgaccagt tgcagcatgt ccgcgtccac
     1861 gtcaccgatt ttgttgttct tgccgaccag accaactttg ttggtcacca acggtgcagg
     1921 aacccgctcc agatccgcgc agattcgttc caggcgggta cgcgcctgca tggtacgcat
     1981 cagttcgctg gtctgtgcct tgttcatccg gccggtcgag atgatcatgt gaaacaccat
     2041 ggctccgagc actccggcaa tgacaatggc cacgattatc tcgacaaggg tcattccgtg
     2101 tatgcgggca cgaagaggca ggcgttgttt tctcatttgc cactccccag ttctctctca
     2161 gcgtgtcggt atgcttgtca gaaatctctg attatgcaat cccatgaaat cttcccaagt
     2221 caccacgagg acaacccgat ccgtgcctgg atcacttttg atcctggagg cacgccatgt
     2281 gtattcaagg ccgcccattg tctgagtccc cttggccaga ccctcctcgt tcaaagggga
     2341 tttggcgcgg ggaagtcgaa ggttgttctt ggcttgctcg gcaacaaagg aggcatttgt
     2401 cagcgtctca gcatagccgg cgtttcgttt cgccgtcgtg aaaagaccgg tcaaaaaacc
     2461 aatcccaatc gcaagaacca gaaccgccaa gagcacatcc accatcgcaa aaccgttctt
     2521 ggagtttttc atagaatcac cctgttttct atagtcccca gaccagacac ctgtctcgct
     2581 tcagcacaaa tcaatgccat cttggtacca taggtttttg tcaattgtca agagaaatgt
     2641 cctcttcaga aatgcacaga ataactcgtg gcgggtggtg ttctccagca agaacgcgag
     2701 ggattattca ctcaaacctt cccagagggc tgttccgatc cgtaccatag tggctccctc
     2761 ctcaatggca atctcgaaat cgttgctcat ccccatggac agaacggaca tctcgattcg
     2821 ggtaatcgcc tggtctcgga tgaagtcggc cagctggcga actcgggtaa agaccggtcg
     2881 cgtctcgtca ggatcggcgg caaagggcgc catggtcatc aaccccttga cgacaattcc
     2941 ctgcatcgtg gcaatgaacc ggcaggcgtc aagggcttcc tgttcggcaa atccaaactt
     3001 ggattgctct cctgaaatgt tcacctcaag cagtatcgga gtctcgcagc cgcgttcgat
     3061 ggaatgccgg tttaactcct cagccagacg aaggctgtct accgaatgaa tgagtgcagc
     3121 cacgggcaga acactcttga ccttgtttct ttgaagatgt ccgatcatgt gccactgaat
     3181 gtccttcagg tcagcaagca tcgaggcttt tccctccagt tgctggggac ggttttcacc
     3241 aaaaattctc agcccgcagg cgtaggcctc gcgtatcatg gcctcagtaa cggttttcga
     3301 gacggcaata agagtgattt ctgaagcatc tcgattcgac cgctctgcgg cgtcgccaat
     3361 tcgtttcatc actgactgaa tgtttcggac aagttgcata gtcaagcctc caaaaactag
     3421 ggagacgcac tgctgcagtc ccgcacccaa taggcagtgc cgttggactc aatctccacg
     3481 gtaccatcca aatctgttct catgacaaga tcacagacag cctcaagtcg tgcaagtgcg
     3541 tccggatgag ggtgaccata cgaattgcgg ccaacctgaa tcaccgccac ttccggagcc
     3601 acctttcgaa ggaaggagct ggatgaagag tacttgctgc cgtgatgcgg caccttgaga
     3661 acttcagatt ccagcacagg caactcatcc aggagcagcc gttcgacctt ctgagtaatg
     3721 tctccagtca acaacagatc gaactcgccg taactggctc gaatcaccaa actggcatcg
     3781 ttgtcttcca ctttcggctg gctcagcacc gtctgactcg gagagacaac ctcaatgttc
     3841 agtccaggga atccttcaac tcggtcgcct gcccgcactc gccgaatgag gatgtcgtgt
     3901 gcctgaagcg cctggagcaa ttgtgcgtag atgacagttg gatcctcaac aggattggtg
     3961 atgaacatgc gaacgggtag actctctaca accgccttaa gctctccaat gtggtcactg
     4021 tccgcatggg tcaaaacaag acagtcaatg cgcttgactc gcaaggcatt cagagcagtc
     4081 aagaggcgaa ttcctcggct ggatgagaag gccttgtcgg gagttccacc gtcaacgaga
     4141 atcgtaccag aacccggtgc ttgaatcagg atgcaatctc cgcttcccag aggcaggaag
     4201 tacaccttga gcgtgctgac gccaccaaag aatccgttca acagcacgac cacaagaaca
     4261 agcacgagca ccacatgaat cttccgcagc gtcagacggg aaaacgtgat ctcaacgtat
     4321 tcctttttgg caaagaagat gaagatcaca ccgtagtacc cgaccagcaa aggccaacct
     4381 ggacgactca tcctcatcgc cgcaaatggc cagtctgcca tccagaagac caaggctcca
     4441 agactgatta gaagatgtcg cacaatccag gcgacgggaa tcgaaatcca ggagtgcagg
     4501 aaacccgcga cggccaggac tccaccaccg acggtgcaga gccataccag aggcattgcc
     4561 gccacattcg ccggcacagt cagcgcattg agtgttccaa agtgccaggc ttggatcgga
     4621 atcagaccca gccacgcaca gaaactcgca caggcaatcc tccaaagata tccaatccct
     4681 ctgattctca t
//
LOCUS       DAOQUC010000109         4671 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_20472_length_4671_cov_4.037695, whole genome shotgun sequence.
ACCESSION   DAOQUC010000109 DAOQUC010000000
VERSION     DAOQUC010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4671
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20472_length_4671_cov_4.037695"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(98..1498)
                     /locus_tag="PKH07_02945"
     CDS             complement(98..1498)
                     /locus_tag="PKH07_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015230131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HNT33935.1"
                     /translation="MQSEHTSSVTDRVDRLRAGFLTGETLSIGLRLRQLRTLYDSIRA
                     NESALLKALHQDLGKPEMEAYLAEIAYVQSEISHALRHLAKWCRPQRRKSPWLAWPSR
                     SFLSPEPYGVVLIIAPWNYPFGLLVSPLVGAIAAGNCVCLKPSEYAPATSSVVAQMLR
                     SIFAEDHVSTMEGDQSVAESLLSERFDYIFFTGSSRVGRRVLEKAAQHLTPVTLELGG
                     KCPCIVCKDAYLETAARRILWGKCMNAGQTCVAPDYVLVDRAIKLPFLSALKETLTRF
                     YGADPMQSKDYGRIVNRKHFQRLIGYLNQGRIVIGGAHDEESLFLEPTVLVDVNPDAP
                     VMQDEIFGPILPVVEFTSLDDTLDALRRAPKPLALYLFTNDRANRQKVLSRTSSGSVC
                     VNDTIVQILGKDLPFGGVGESGMGNYHGKASFDCFTHYKSVMAKPSGLDFSFRYPPTS
                     PSWERLKRIYEFLMRR"
     gene            1735..3228
                     /gene="crtI"
                     /locus_tag="PKH07_02950"
     CDS             1735..3228
                     /gene="crtI"
                     /locus_tag="PKH07_02950"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007721026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016166 - phytoene dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HNT33936.1"
                     /translation="MTNCNNREVVVVGSGLGGLSAAISLAQEGYSVEIHEKNSKIGGK
                     LNFLSEQGYTFDLGPSILTLPHFFERLFARSGRKMADYFSIVAVRPHWRSFFPDGKTI
                     DLYPEPELMAREAVKAGASAKSVECFLDYSARLYDLIDRGYFQEGLDSTRDILRFYGW
                     CALPRFDPLRSMHSGVARHIQNPYLKDIFDFFVKYVGSSAYRAPAMLNCLPTIQFRYD
                     LWYVNGGMYNIARGLQKLLGELGVAVHLNSEVTEFRTSQGRVTGIVVNGDRFHKADIV
                     VCNMEVIPAYRDILKEDATFLRSLRKFQPACSGLVIDLGLDIQYPHLAHHNFFFSGDQ
                     QKHFDSVFRKKVLPEDPTIYLVAASRTDPTVAPAGTDCLKILPHIPPIDPEHPLQLDD
                     YTRLRDRILIKLEKMGLTDLRKHIVFEHFWTPYDIQKQYYSNQGSIYGVVSDRYLNFA
                     FKAPKHSSRYDNLFFVGGSVNPGAGMPMVVLCGQNVCRDIVRWDQLT"
     gene            3298..>4671
                     /locus_tag="PKH07_02955"
     CDS             3298..>4671
                     /locus_tag="PKH07_02955"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNT33937.1"
                     /translation="MELTFLGAVRQVTGSCFVLKAAGLKMMVDCGLFQERCCLARNWD
                     LTDITANEIDYLLLTHAHLDHSGRIPRLVSQGYRNPIIATEATADLTKIVLEDAARLQ
                     TEDTEFKKERHKREGRTGPHPVVPLYTEEDVEMVLPLIQRKPYGTPVHLSDKVTIQFH
                     DAGHILGSSILDISVWTGEKPQRILFSGDLGQWDRPLVRDPSIFEQADYIVLESTYGD
                     RDHEVTGDVKDQLAQVINDTVERGGNIVIPTFAIERAQELMYHLSELVREDRIPHLMV
                     FLDSPMAVNVTRVFEKHLDLLDKDTAAKYRQGEPPFEFPGLKLVRHAGESKAVNRIKG
                     SCIIMAGSGMCTGGRIKHHLSKNIIRPESTILFVGYQSRETLGRQILDGEPEVRIHGQ
                     MHPVRARIAQIHGLSAHADRTALLRWLSYFKTPPKCLYMVHGEEEAALAFADQVRKQT
                     GWNVAVPA"
BASE COUNT         1122 a         1209 c         1237 g         1103 t
ORIGIN      
        1 ccaggaagcg gcgtgccgac gatccttgaa tctggaagga tcacctcaga catgctgaca
       61 cgaagataca agcgcacgta gtctttcgcc gtcgcggtca gcgtcgcatc aggaattcgt
      121 agatccgttt gagcctctcc catgagggtg acgtgggagg atagcgaaaa gagaaatcca
      181 aacccgaagg ctttgccatg acggatttgt agtgcgtgaa acagtcaaag cttgccttgc
      241 catgatagtt gcccatgcca ctttccccaa cgcctccaaa cggcaagtcc tttccaagaa
      301 tttgaactat cgtatcgttg acacaaacac ttcctgaaga ggttctcgaa agaaccttct
      361 gacgattggc tctgtcgttg gtgaaaaggt acagcgccaa gggtttgggc gcacggcgca
      421 gggcatcaag cgtgtcgtca aggctggtaa actcgacaac aggaagaatc ggaccaaaga
      481 tttcgtcctg catgacggga gcgtccggat tcacatccac aaggactgtc ggctccagaa
      541 agagactctc ttcgtcgtgc gcaccaccaa tgacgattcg tccttgattg agatagccaa
      601 tcagacgttg gaagtgtttg cgattgacta tcctgccgta atctttgctc tgcattggat
      661 ccgctccata gaagcgagtc aaagtctctt tcaacgcact cagaaagggc agtttgattg
      721 ccctgtccac aagaacatag tctggcgcaa cgcatgtctg ccctgcattc atacattttc
      781 cccagaggat gcgcctggca gccgtttcga ggtaggcatc cttacagaca atgcacgggc
      841 atttgccacc caactcaagc gtcaccggag tcaggtgttg cgctgccttc tccaataccc
      901 tgcgaccgac tcgactgctg ccggtgaaga agatgtaatc gaagcgctcg gagagaaggg
      961 attcggcaac ggactggtcg ccttccatgg tcgaaacatg gtcttcggca aagattgaac
     1021 gaagcatctg agccacgact gatgaagttg ccggagcgta ttccgatggt ttcagacaga
     1081 cacagtttcc agcagcgatt gctccaacca aaggagacac caacaggccg aaaggatagt
     1141 tccatggtgc gatgatcaag acaacaccgt acggctcggg agaaaggaaa cttcgcgaag
     1201 gccatgccag ccaaggactc ttccgccgct gcggacggca ccatttcgcc agatggcgta
     1261 gagcgtgcga tatctcgctt tgtacatagg caatctcggc aagataggct tccatctcag
     1321 gtttgcccaa gtcctgatgg agcgctttga gtagggcgga ctcattggct cgaatgctgt
     1381 cgtagagtgt cctgagttgc ctgaggcgga gacctattga aagagtctca ccggtcagga
     1441 agcctgctct caaacgatca accctatctg tgacactgct ggtgtgttcc gactgcatcc
     1501 tgcactcttg tgagtcttcg ttgatcactg acctgtaccc tcaacatgac gttagagact
     1561 gagaacacca tagaccgatt attccggctt ggcaaaacgc ctcattactt gacacctcga
     1621 acgcagatca tcagaataat gaaacaagtg acagacaatc cttgctgcgc aggctgttgt
     1681 tggattggtc ggcacgccag agtctgccac agaatcgagg aaccgaagaa tacgatgaca
     1741 aactgcaaca acagagaggt agtcgttgtc ggatcgggtc tcggtggact ttccgccgcc
     1801 atctccctgg ctcaggaagg ttactcggtc gaaatccacg agaagaactc taagatcggt
     1861 ggaaaactca atttcctcag tgaacaagga tacacctttg atctcggtcc ctcgattctg
     1921 actctgcccc atttctttga gcggctcttt gcgcgttcgg gacgaaagat ggctgattac
     1981 ttctcgatcg tggcagtgcg tccacactgg cgatcctttt tccccgatgg aaaaaccatt
     2041 gatctttatc ccgaacctga gttgatggct cgtgaagcag tcaaagcggg agcatcggcc
     2101 aagtccgtgg agtgtttcct ggattactct gccagactct atgatttgat tgacagagga
     2161 tactttcagg aaggtctgga cagcactcgg gacattctgc gattctatgg atggtgcgca
     2221 cttcccagat ttgacccgtt gagaagcatg cactccggtg ttgcccggca cattcagaat
     2281 ccctatctta aggacatctt tgacttcttt gtgaagtacg tgggttcatc tgcttaccga
     2341 gctcctgcca tgctgaactg tcttccgacg atccagttcc gatacgatct ctggtatgtc
     2401 aacggcggga tgtacaacat tgcccgaggc ctgcaaaaac tcctggggga acttggcgtt
     2461 gcagtgcacc tgaacagcga agtcaccgag ttccgaacct cgcagggccg tgtcacgggg
     2521 attgttgtga acggtgacag attccataag gcggatatcg tggtctgcaa catggaagtc
     2581 attcccgcct atcgggacat tctcaaggaa gatgcaacgt tcctcagaag tcttcgaaag
     2641 tttcaaccag cctgttcggg actggtcatt gatctcggcc tggacattca gtatccgcat
     2701 ctcgcacatc acaatttctt cttctcaggc gatcagcaaa agcattttga ttctgtgttt
     2761 cgcaagaagg ttctgcccga agatccaaca atctatcttg tcgctgcttc gcgcacggat
     2821 ccaacggtcg ctcctgcagg aacggattgt ctcaagattc tgcctcatat tccgcccatc
     2881 gatccagagc atccactgca actggatgac tacacacgcc tcagagatcg gattctgatc
     2941 aaactggaga agatgggctt gacggatctt cggaaacaca tcgtctttga gcatttctgg
     3001 acaccgtacg atattcagaa gcagtactac tccaatcagg gctcaatcta tggtgttgtc
     3061 tctgaccgat atctcaattt cgctttcaaa gcgcccaagc acagttcccg ctatgacaat
     3121 ctattcttcg tgggtggatc cgtgaatccg ggggcgggaa tgccgatggt ggtactttgc
     3181 ggtcagaatg tttgccgaga tatcgttcgc tgggatcagt tgacatgagt tcctcaagcc
     3241 ccgtacactt cctttgattg gttcttgcga agacacacaa actggagagg tatcccgatg
     3301 gaactgactt ttctcggtgc cgttcggcag gtgactggat cgtgctttgt gctcaaagcg
     3361 gcggggctaa agatgatggt ggactgcggt ctattccagg aacgctgttg tctggcacgc
     3421 aactgggact tgacggacat taccgccaac gagattgact atttgcttct gactcatgcg
     3481 catttggatc attcgggtcg aatccctcgg ttggtcagcc agggataccg caatccgatc
     3541 atcgctacgg aagccacggc cgatctcaca aagatcgttc tggaagacgc cgcccggctg
     3601 cagacagaag atacggagtt caagaaagag cgtcacaaac gagaaggccg aaccggtccg
     3661 catcctgtcg ttcctttgta taccgaagag gacgttgaga tggttcttcc cctgattcag
     3721 cgcaagccct atggcacgcc ggtgcatctg agtgacaaag taaccataca gtttcacgac
     3781 gcagggcaca ttctcggttc ctcgatactg gacatctccg tttggactgg ggagaaacct
     3841 caacggattc tgttctctgg cgatctgggt caatgggaca ggcctctggt gcgcgatccg
     3901 tcgatctttg agcaggccga ctatattgtg ctggaatcca catacggaga ccgggatcac
     3961 gaagtgacag gggacgtcaa ggatcagttg gctcaggtga tcaacgatac ggtggaacgc
     4021 ggcggaaaca ttgtgatccc gacgtttgcc attgagcgcg cgcaggagct tatgtaccac
     4081 ttgagtgaac tggttcgaga agatcgaatt ccgcacttga tggtgttcct tgacagccca
     4141 atggcagtca atgtaacacg ggtcttcgaa aagcaccttg accttctcga taaagacacg
     4201 gccgcaaagt atcgccaagg ggagccgcct tttgagtttc caggcttgaa actggttcgg
     4261 catgcaggag agtccaaagc agtcaatcga attaagggct cctgtatcat catggcaggc
     4321 agcggcatgt gtacgggtgg acgcatcaag catcatctgt ccaagaatat aatccggcct
     4381 gagtccacca ttctttttgt gggctatcag tcacgagaaa ccttggggcg acagattctg
     4441 gatggagagc ccgaagtccg aattcatggt cagatgcatc cggtcagggc gcgaattgcc
     4501 cagattcatg gcctgtctgc gcatgcagac cgaaccgcct tgctgcggtg gttgagttac
     4561 ttcaagacac cgcccaagtg tctctatatg gtgcatggtg aagaagaagc ggccttggca
     4621 ttcgcggatc aagttcgaaa acagaccgga tggaacgttg cagttcctgc g
//
LOCUS       DAOQUC010000110         4669 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_20484_length_4669_cov_2.786303, whole genome shotgun sequence.
ACCESSION   DAOQUC010000110 DAOQUC010000000
VERSION     DAOQUC010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4669)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4669)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4669
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20484_length_4669_cov_2.786303"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..929
                     /locus_tag="PKH07_02960"
     CDS             <1..929
                     /locus_tag="PKH07_02960"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HNT33938.1"
                     /translation="YRDHRFDPNVDRQTGFRSRSILCVPLQIGNKRTGVVQVLNRCDG
                     EPFTRDDLSLLELVASFIALAIENAREHQMRVEAQRLAVIGQTVSQLGHCIKNALQGL
                     QGGSYLLDKHLSDHDASARTRAWAMLQRHISFLSDLVMNMLSYAKKRNPVCEMANVNV
                     FCKDVAEMISAHAASQGVVVSFDLSEEMELVPLDGAGIKRCLYNLVTNAIDACESGKG
                     QVVISTGCQQEDSGVFVSIQDNGVGIPPESLSRLCEAFYSTKGNRGTGLGLSVCKKII
                     EEHGGRIQFDSTVGKGTKVTLLLPNTAPTAGT"
     gene            1036..1425
                     /locus_tag="PKH07_02965"
     CDS             1036..1425
                     /locus_tag="PKH07_02965"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNT33939.1"
                     /translation="MDDEEDCRLYVRSILEDEGFSVHEADCGCAAASKAREIQPALVI
                     LDIQMPDKNGLEVISDLRLQQESATVPVVMLTGIADQTGLRFSPEEMEDWVGEKPEAY
                     LEKPVSPNELKRTVRRILEDRGFLKAN"
     gene            1469..2881
                     /locus_tag="PKH07_02970"
     CDS             1469..2881
                     /locus_tag="PKH07_02970"
                     /inference="COORDINATES: protein motif:HMM:NF018012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HNT33940.1"
                     /translation="MIPLMKLEVLDLQEMQQIHSAALRILTRTGVQVEHEGLCEKLSD
                     FGASLYRGDQRVRFPESLVERFLAESTAFDYTSVRPSVTGQAGVYQGLYLEPESNSLV
                     HFTEKTLADYVKLARLLDNVTGIHILNYPVAAGSPTEPLELLVFAWKYGADVSGSIQQ
                     TFLCPYLFEMYSIQAETTGKSLEEVFRGGVFLISPLRFPAHEAEQLMYFHDKGLRVWM
                     ANMITAGGTGPVTLAGCVALNIAEAIFMGIVNRTLFGDREWRLDCNIAPIDMRTMIQP
                     YGRPEMLLANLATLQMARYYRVAGGGHCGLTDAKIPSQEAGSQKLLTALPCVLAGGGN
                     IEPGLLSIDEVFSPIQMIIDAEVVGAIRRVLAGFEVTEETLAVDVINAIGPGGFFTAT
                     EHTVAHFRKEQWEPSVWSREMVQTWLSQGGKTDVDRALNIWKDIMTKPDLEPGITPET
                     EHRLRSVIEQAKRGISKKAV"
     gene            2909..4150
                     /locus_tag="PKH07_02975"
     CDS             2909..4150
                     /locus_tag="PKH07_02975"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNT33941.1"
                     /translation="MRPIVECTNVTKSYAPREHLSNLSGWQAWCRLRMGTRRREPFDA
                     LKNVSFSVHPGEAVAVIGENGSGKTTLLKLIAGISEPSAGSIAVGGRVGSLLEVGAGF
                     HPEMTGRENIFLSGAILGMTRKQLEERVEQIAQFSELDEFLDVPVKFYSSGMFVRLGF
                     SVAIHCLPEVLLLDEILAVGDEHFQKKCIRAIEAFLQDGGTILYVSHDFRSVRMLCPR
                     ALVLSDSELIFEGETEGAISKYHHRLWQKHHVGPGIPTETVYRNRFGNFDARILNVRI
                     LDGQGSSKREFDIGDTLQVEVDYRVERPIGESTLGLITRDTGGNLLHSCSTVLMGTEI
                     PTTIGEHRVRFGLSQMNLRVGLYDITVALSSSIVSTHFPHDWRKYIYDIWYRMDTFQI
                     LPSQMEAKRCDGFADIGVHWA"
     gene            complement(4158..>4669)
                     /locus_tag="PKH07_02980"
     CDS             complement(4158..>4669)
                     /locus_tag="PKH07_02980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33942.1"
                     /translation="TLGLGAIVGALILSWWGHGHPREKLIGVSFFLTGCLSLPFVWSL
                     VSGAREGTMQAFLKMDLMALLAGVFVAPIMIASDTWLQEIVPEHLRGRIFGSKETLLA
                     FAFIGGNMLIGYIAEDMTSIQRDMLLYGVAAVLMLLGFTWALLVFWHSPHSHSGTSGS
                     QASESTRTT"
BASE COUNT         1141 a         1172 c         1313 g         1043 t
ORIGIN      
        1 cctatcgcga ccaccggttt gatccgaatg tcgatcggca aacgggattt cgaagtcgct
       61 cgatcctctg cgttcccctt cagattggca acaagcgaac cggagtggtt caggtgttga
      121 accgctgtga cggagaaccg ttcacacgtg atgacctgag tttgcttgaa ctggtggcct
      181 cttttattgc gctggcgatc gagaatgccc gagagcacca aatgcgggtc gaagcccaaa
      241 ggcttgcagt cattggtcag accgtttccc aacttgggca ttgcattaag aacgctctcc
      301 agggtcttca gggtggatct taccttctgg acaagcatct ctccgatcac gatgccagcg
      361 cccgaacaag ggcttgggca atgcttcaac gccacatcag tttcctgagt gatctggtga
      421 tgaacatgct ttcgtacgcc aaaaagcgaa accccgtttg cgaaatggca aatgtcaacg
      481 tattctgcaa ggacgttgcc gagatgatct ccgcgcatgc ggcgagccaa ggtgtggtgg
      541 tgtcgttcga tctgtccgag gaaatggaac ttgtgccttt ggatggagca ggaatcaagc
      601 gatgcctcta caatctggtc accaacgcca ttgacgcctg tgaatctgga aaaggtcaag
      661 tggtcatctc gaccggttgc cagcaggaag actcgggcgt ttttgtgtct attcaggaca
      721 acggggtcgg cattcccccg gagagcctct cgcggctttg cgaagcattt tacagcacca
      781 aggggaatcg tggtaccgga ctgggtcttt cggtctgcaa aaaaatcatc gaggaacatg
      841 gaggcagaat tcagtttgat tcaacggtcg gaaaggggac gaaggtgacg ctacttcttc
      901 ccaacactgc accgaccgcg ggtacctgat tctttcgaga cggaatacgt cactgcccga
      961 tctgcattca aatctgagtt cacaagttag agcaatatga ccacagaggg aaaagaacct
     1021 atcaggattc tggttgtgga cgatgaagag gactgccgac tgtacgtccg cagcatcctt
     1081 gaagatgaag ggttttccgt tcatgaggcg gactgcggct gtgcggctgc ctcaaaggca
     1141 cgagagattc agcctgcact cgtcatcctc gacattcaga tgcccgataa gaacggcctt
     1201 gaggtgatct ctgatctccg tctgcaacag gagtccgcca cagtgcctgt ggtaatgctg
     1261 accggcattg ctgaccagac aggattgcgt ttctctccag aggagatgga agactgggtg
     1321 ggtgagaagc cggaagccta tctcgagaaa ccggtcagtc cgaatgaact caagagaacc
     1381 gtccggcgca tcctcgaaga ccggggattt ctgaaagcaa actgatccac ttcgggtcaa
     1441 cagcaccgcg cgtgcagaag gaggcacaat gattcccttg atgaaactgg aggtattgga
     1501 tctccaggag atgcagcaga tccattcggc agcactgcgg atactcaccc gcacaggtgt
     1561 ccaagtggaa catgagggac tctgtgaaaa gctgtcggac ttcggtgcgt cgctctacag
     1621 aggcgatcag cgagttcgat ttcctgaatc actcgtcgag cgttttcttg ctgagagcac
     1681 tgcctttgat tacacctcag tcagaccttc agtgaccgga caggcaggag tctatcaggg
     1741 actttatctc gagcctgaga gcaatagcct ggttcatttc accgagaaga cacttgccga
     1801 ctacgtcaaa ctggctcgtc tactggacaa cgtaaccggc attcacattc tgaactatcc
     1861 ggtcgcggca ggcagtccca ctgagccgct tgaacttctg gtctttgcct ggaaatacgg
     1921 agcagacgtt tcggggagta ttcaacagac attcctgtgc ccctatctct tcgaaatgta
     1981 ctcaattcag gcggaaacca caggaaagtc tctggaagag gtcttccgag gcggtgtgtt
     2041 tctgatcagt cctttgcgct ttcccgcaca tgaagccgaa cagctgatgt acttccatga
     2101 caagggactg cgcgtctgga tggccaatat gattaccgcc ggaggcactg gaccggtgac
     2161 ccttgctgga tgcgttgctt tgaacattgc cgaagcgatc ttcatgggaa ttgtcaaccg
     2221 aacgcttttc ggtgacaggg aatggcgact ggactgcaac attgctccga tagacatgcg
     2281 tacgatgatt cagccgtatg ggcgacccga aatgctcttg gcaaacctgg caacactgca
     2341 gatggcacgg tattaccgtg ttgctggtgg agggcactgt ggcctgacgg atgcaaagat
     2401 tccatcacag gaagcaggat cgcagaaact gcttacagct ctgccatgtg ttctggcggg
     2461 aggcggcaac attgaacccg ggctgctttc cattgacgag gtcttttcac cgatccagat
     2521 gatcattgac gccgaagtgg tgggagcaat acgaagggtg cttgcgggtt ttgaagtcac
     2581 tgaagagact ttggcggtgg acgtgatcaa cgcgattggc ccgggaggtt tcttcacagc
     2641 gacagagcat acagtcgccc attttcgaaa agagcagtgg gaaccaagcg tatggtctcg
     2701 ggagatggtt cagacatggt tgagccaggg tggaaagact gatgtggatc gcgccttgaa
     2761 catctggaaa gatatcatga caaagcccga cctcgaacct gggatcacac ccgaaacaga
     2821 gcatcggctt cggagtgtca ttgaacaggc aaaacgaggg atcagcaaga aggcagtcta
     2881 gagtcagtcc aagagagacg gtatctcaat gcgtccaatc gtcgagtgta cgaacgtcac
     2941 caagagctac gccccgcggg agcacctttc gaatctcagc ggttggcaag cgtggtgccg
     3001 ccttcgaatg ggaacacgaa gacgcgaacc atttgacgca ctcaagaacg tctccttcag
     3061 tgttcatcct ggggaggccg ttgcagtcat cggcgaaaac ggctccggca agacgactct
     3121 tctgaagttg attgcgggaa tcagcgaacc gagtgcggga agcatagccg ttggaggtcg
     3181 cgtgggaagc cttctggaag tgggcgcggg gttccatcct gagatgacag gcagagagaa
     3241 tatctttctc tctggagcga ttctcgggat gacccgaaag caactggaag aacgtgttga
     3301 gcagatcgcc cagttttccg aactcgatga gtttctcgat gttccggtga agttctactc
     3361 atcggggatg tttgtccggt tgggtttttc ggtagcgatc cattgtcttc ctgaagtgct
     3421 cttgctggat gagatcctgg cggttggcga tgagcacttt cagaagaagt gcattcgagc
     3481 cattgaggcc tttctccaag atgggggaac aatcctgtat gtctctcatg attttcgttc
     3541 tgtccgcatg ttgtgtccga gggctttggt tctcagtgat tccgaactga ttttcgaggg
     3601 ggagacggaa ggtgcgattt caaaatatca ccaccgactc tggcaaaaac atcacgtcgg
     3661 gccaggaatt ccaacagaaa cagtttaccg gaatcggttt gggaacttcg atgcgagaat
     3721 tctcaatgtc cgaatccttg atggccaagg atcaagcaag cgggaattcg acatcggcga
     3781 cacgcttcag gttgaggttg actaccgggt tgaaaggccg attggcgaat ccacacttgg
     3841 tttgattact cgcgacacgg gaggaaactt gctccactca tgcagcacgg tgctcatggg
     3901 aacagagatc ccgacgacga ttggagagca tcgggttcgg tttgggctga gtcagatgaa
     3961 cctgagagtt ggactctacg atatcacggt cgctttgagc tcaagcatag tcagtaccca
     4021 ctttccacat gactggagaa agtacatcta cgacatctgg tatcgcatgg acaccttcca
     4081 gattctccca agccagatgg aggcaaagag gtgtgacggt tttgctgaca ttggcgtgca
     4141 ttgggcttaa cgcctcttca tgttgtccgt gtggactcag atgcttggga acccgacgta
     4201 ccggaatgtg aatgaggcga gtgccagaac acaagaagcg cccaggtaaa acccaggagc
     4261 atcaagaccg ccgcaacgcc gtaaagaagc atatcccgct gaatactggt catatcctca
     4321 gcaatgtacc caatgagcat gttacctccg ataaaggcga aggcaagaag cgtttccttg
     4381 cttccaaaaa tgcgaccgcg aagatgctct ggaacgattt cctgaagcca cgtatctgag
     4441 gcgatcatga tcggcgcgac aaagacgcct gccaacagtg ccatcaaatc catcttcaga
     4501 aacgcctgca tcgttccctc tcgcgcgccg gagaccagag accagacaaa gggaagactc
     4561 agacaaccgg tcaggaagaa tgagacccca atgagtttct ctctcggatg accgtgtccc
     4621 caccaggaaa ggatcagagc tcccactatc gctcccagac cgagagttg
//
LOCUS       DAOQUC010000111         4656 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_20552_length_4656_cov_3.194306, whole genome shotgun sequence.
ACCESSION   DAOQUC010000111 DAOQUC010000000
VERSION     DAOQUC010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4656)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4656)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4656
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20552_length_4656_cov_3.194306"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(31..372)
                     /locus_tag="PKH07_02985"
     CDS             complement(31..372)
                     /locus_tag="PKH07_02985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33943.1"
                     /translation="MRLMDIQSVMQNARVVEQAQAQVQQAQEFAKTLLAQEDQNERQQ
                     QLSTVRNIKETDNPRVESEPRRQRGASGGQTGAQEREQRHASTQQDKDQGNPPAGETD
                     LEEGHHIDIVV"
     gene            complement(407..1192)
                     /locus_tag="PKH07_02990"
     CDS             complement(407..1192)
                     /locus_tag="PKH07_02990"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HNT33944.1"
                     /translation="MTESRTAVNSTDPQTKSPMFATQDERADFIKSKLHLVNYVLARL
                     VDTSLIGKGVLDFDDLYSSGVVGLIEAADHYDPTREAKFSTFAVPRIRGSILDAIRTN
                     DPVSRTIRKRSRMLQKAYSELEQNLQRPASDEEVAEKAGMSLGEMHEVLQILASVPTV
                     SMDESLSVDDESGVELHESVEDPKTVDPRHATHRTEMEERLLESMRDLPERERTMMAM
                     YYFDEMTFREIGIVLGVTESRVCQMHTRILLRLKSKLKRFRFV"
     gene            complement(1246..1740)
                     /locus_tag="PKH07_02995"
     CDS             complement(1246..1740)
                     /locus_tag="PKH07_02995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33945.1"
                     /translation="MDEIELQEHREQMLALRKRLMVTMATAGMLCVMIASVLASADSM
                     TILARSTMAAFGFGMIGYVVGTVIAMANSTPPTSNEDSEDESGSGVVQVDGQNVEIVT
                     CWVPVLDVRPGMRLSEDIPDPGTQEGAVLIPARTVLQPSDIERLKKMRVPTIPVAGLS
                     FKQP"
     gene            complement(1783..2673)
                     /locus_tag="PKH07_03000"
     CDS             complement(1783..2673)
                     /locus_tag="PKH07_03000"
                     /inference="COORDINATES: protein motif:HMM:NF012369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HNT33946.1"
                     /translation="MSGVDQATKLRELIKQKEETLPPIETREPSRIIAVTSGKGGVGK
                     SNIASNLAIAFAQAGKRVLIIDADLSLANVDILLDVHPRFNLSHVIFGERKLEEVLVE
                     GPEGVRILPASSGVTELANLGHAQLEGLINQFSDLADETDILIIDTAAGISENVLAFV
                     AAAREALVVTTPEPTAYTDAYAMLKVLSTRQVEVQLGLVVNMAGDEREAAQAGENMVL
                     VCRQFMNMGIRYWGHVLKDPDVGAAIRRQEPFILYAPHCHASRCLRTLAANLLESHNG
                     SVPRPRKGFWSRFVEHLRIK"
     gene            complement(2678..3916)
                     /gene="flhF"
                     /locus_tag="PKH07_03005"
     CDS             complement(2678..3916)
                     /gene="flhF"
                     /locus_tag="PKH07_03005"
                     /inference="COORDINATES: protein motif:HMM:TIGR03499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HNT33947.1"
                     /translation="MRVKKYVAEELHEALRQLRAELGPEAMILQTKPCEIKRFFGLIR
                     RTGVEVTAGVGMNFLTDLVQRKPETSRPVAEQPSGEPAIAKPAAPTVSDEQVKTPEVI
                     SLEERMKRLEAGMLQIQDVLGNIRQPHSPGDEVELSVPTPVQNLFRQLCVLGFTEDYA
                     LQLTDALKRHGNGSATLEMLQEHLIGTLTASLSCVEPLQAIPQSSKVIALIGPTGVGK
                     TTTLAKLSASFCLHSNKRIAMITTDTYRLAATDQLQRYADILGVPLEIVYTPEEMRQA
                     IDRLQDVDVIFVDTAGRSQKNLEQMSDLKRFLEAASPAQVHLLLSATTKYDDLMDVIT
                     RFELVPLSSLIITKTDESNSFGAIASLLKEVSLPVSYLTTGQNVPEDIEKATPDRLAR
                     LFVLGLNEAGRTGHNTGNEG"
     gene            complement(4031..>4656)
                     /locus_tag="PKH07_03010"
     CDS             complement(4031..>4656)
                     /locus_tag="PKH07_03010"
                     /inference="COORDINATES: protein motif:HMM:NF012973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
                     /protein_id="HNT33948.1"
                     /translation="HAPDLLDRQAVQRLIDRLRETHTVLVNELVPDLLTVGQIQKVLQ
                     MLLRERVCIRDLGTVLEALADAAHATRDIAQLTELVRTALARPLTQSFLAPDGNLYVM
                     TLEPRLEQTLVEAIQKSTEGTYLTLDPQVSRKLLERIGEEMEKVAARGFQTVLLTNPQ
                     LRPHLHALVERFLPHLVVMSYTEVASDAKVQSSGVVRWEPAAQEARR"
BASE COUNT         1005 a         1406 c         1160 g         1085 t
ORIGIN      
        1 ggcttaccag gaaagatccc gacgtctctg tcaaacaaca atatcaatgt gatgaccttc
       61 ctctaaatcg gtctcacccg caggcgggtt gccctgatct ttgtcctgct gtgtagatgc
      121 gtgcctttgc tctctctcct gtgctcctgt ctgtcctccc gaggctccgc gttggcggcg
      181 aggttcagac tcaacacgcg ggttgtctgt ctccttgata tttcggactg tgctcaactg
      241 ctgttggcgt tcgttctgat cctcttgagc caaaagggtc ttggcaaatt cctgtgcttg
      301 ttgaacctgc gcctgcgcct gctcaacgac acgggcattt tgcatgacac tttgaatgtc
      361 catgagtctc atttgcccga tcctccagcc ctgtccgact tccggtctac acgaacctaa
      421 atcgcttcaa cttcgatttc agccgtaaca gaatcctggt atgcatctgg cacacacgcg
      481 attccgtcac tcctagaacg attccgatct cccgaaaggt catttcgtcg aaataataca
      541 tcgccatcat cgtgcgttcc cgttccggca ggtcacgcat ggattccaga agacgctctt
      601 ccatctccgt cctgtgcgtc gcgtgacgcg gatcaactgt tttgggatcc tcgacgctct
      661 catgcagttc cacgccagac tcatcatcaa cagacaacga ctcgtccatt gagaccgtgg
      721 gaacggacgc gagaatttga agcacttcgt gcatctcacc caacgacata cctgctttct
      781 ctgcgacttc ttcatccgag gcggggcgct gcagattctg ttccagttca gagtaggctt
      841 tctgaagcat tctgctgcgt tttcggatcg tgcgagagac cggatcgttc gtccggatgg
      901 cgtcgagaat ggaacctcgg atccggggaa cggcaaaggt ggaaaacttt gcttcccggg
      961 tgggatcata gtggtctgcg gcctcgatca gcccgacgac tccgctcgaa taaaggtcat
     1021 cgaaatccaa cactcctttt ccaatcaacg atgtatccac cagccgtgcc aacacgtagt
     1081 tgaccaggtg cagcttgctc ttgatgaagt cagcccgctc atcctgagtg gcaaacatcg
     1141 gactcttcgt ctgcgggtct gtcgagttca ccgctgtcct actctctgtc atcggcattt
     1201 ctccgacgtt acttgaaaat caggcacttg cattcacagt cctcttcacg gttgcttgaa
     1261 cgacaaaccg gcaaccggaa ttgtcggcac acgcatcttc ttgagcctct cgatatccga
     1321 tggttgaagg acagtccgtg cgggtatcaa cacggcgcct tcctgtgtcc ctgggtcggg
     1381 aatgtcttcc gaaagcctca tccccggtcg gacatccaga actggcaccc aacacgtaac
     1441 gatctcaacg ttctgcccat ctacttgcac aacacccgaa cctgactcgt cctcactgtc
     1501 ctcattcgaa gtcggcggcg tggaattcgc catcgcgatg accgtaccga ccacataacc
     1561 gatcatgccg aatccaaagg cggccatagt ggagcgggcg agaatggtca ttgaatcggc
     1621 cgaagccaag acactcgcga tcatcacgca cagcatccct gctgtggcca ttgtaaccat
     1681 cagccgcttt ctgagagcca gcatctgctc ccgatgctct tgcagttcga tctcatccat
     1741 gttcaacgta tcatcaccgc ctgaatcgtt ccatcaaaag cactacttga tccgcagatg
     1801 ctccacaaaa cgagaccaga atccctttcg cggtctcggc accgatccat tatgactctc
     1861 caggagattc gccgcaagcg ttctcaaaca ccgcgaggca tggcaatgag gcgcatagag
     1921 aataaacggc tcctgtcggc gtatcgcggc cccgacatcg ggatccttga gcacatgacc
     1981 ccaataacga atacccatgt tcatgaactg ccgacagacc agcaccatgt tttcacccgc
     2041 ttgtgccgcc tcacgttcat cgccagccat gttgaccacc aagccaagtt gcacttcgac
     2101 ctgacgggtt gagagcactt tgagcattgc ataggcatcc gtgtaggcgg tgggctcagg
     2161 tgtggtcaca acgagcgcct cacgagctgc cgccacaaac gccaagacgt tttcagaaat
     2221 acccgcagcc gtgtcaatga tcagaatgtc cgtctcatcc gcgagatcgg aaaactggtt
     2281 gatcaacccc tccaattgag catgacccag gttggccaac tcagtcactc cagaactggc
     2341 gggaagaatc ctgacgcctt ccggcccttc aaccagcact tcctcgagtt tgcgctcacc
     2401 aaagatcacg tgtgagaggt tgaaccgcgg atgcacatcc agcaggatgt ccacattggc
     2461 caatgagaga tccgcatcaa tgatcaacac gcgtttgccg gcttgtgcaa aggcaatagc
     2521 aagattggag gcaatgttgc ttttgcccac accacccttg ccactggtga cggcaataat
     2581 gcgcgacggc tcgcgggttt cgatcggcgg aagggtttcc tccttctgct tgatcaactc
     2641 gcgcaacttt gtcgcctgat ccactcccga cattcgacta tccttcgttc ccagtattgt
     2701 gaccggtgcg accggcttca ttcagtccaa gcacaaaaag ccttgccagc cgatccggcg
     2761 ttgccttctc gatgtcttca ggtacattct gaccggttgt cagataggaa accggaagcg
     2821 atacctcttt cagaaggctg gcaatcgctc cgaaactgtt tgactcatcc gtcttggtga
     2881 tgatcaggct ggacaaaggc accagttcaa agcgcgtgat gacgtccatt aggtcatcat
     2941 atttcgtggt ggccgaaagc aacagatgta cctgagcggg tgaggccgct tccaaaaatc
     3001 tcttcagatc gctcatctgc tccaggttct tctgactgcg tccggcggtg tccacgaaga
     3061 tcacatcaac gtcctgaagg cgatcaatgg cttgtcgcat ttcctccggc gtgtagacaa
     3121 tctccagcgg tacaccaagg atgtcggcgt agcgctgcag ctgatctgtt gcggccaagc
     3181 ggtacgtgtc tgtcgtgatc atcgcaatgc gtttgttgga gtgcagacag aaagatgccg
     3241 agagctttgc cagggttgtc gtcttgccaa ctccagtcgg tccgatgagc gcgatgacct
     3301 tcgacgattg ggggattgcc tgaagaggct ccacacagga cagggacgca gtcaaggttc
     3361 cgatcaagtg ctcctgaagc atctccagtg tggcacttcc gttcccgtgt cttttcaggg
     3421 cgtccgtcag ctgcaatgca tagtcctctg tgaaaccgag aacacacaac tgtcggaaaa
     3481 gattctgaac cggcgtgggc actgacagct caacctcgtc cccaggagaa tgcggttgcc
     3541 ggatgttgcc caacacatct tgaatctgga gcattccagc ctccaggcgc ttcatgcgtt
     3601 cttcaaggct tatcacttcc ggagtcttta cttgctcgtc cgacaccgtc ggagcagcgg
     3661 gcttggcaat ggcaggttcc ccggaaggtt gttcggcaac gggacgcgag gtctcgggtt
     3721 ttcgctgaac gagatcagtc aggaagttca tcccaacacc cgccgtgact tcaacacccg
     3781 tgcggcggat caggccaaag aatctcttga tctcgcaggg cttcgtctga aggatcatcg
     3841 cctctggacc caattccgca cgcagttgtc tgagcgcctc atggagttcc tccgcaacgt
     3901 acttcttgac tctcatcgct ttccgtccat cccgtctccg agccactgtc cgctggctgg
     3961 cctttgggca atacctacgc taccgaacac ttgacctgat ccgaaatcct ggcttttcca
     4021 caactcgacc ttaccgtctc gcttcctgag ctgccggctc ccagcgaacc actcccgaac
     4081 tctgtacctt ggcatcagat gccacttcag tgtacgacat aacgacaagg tgtggcaaga
     4141 aacgctccac caaagcgtgc aaatggggac gcagctgggg attggtcaac agcaccgtct
     4201 gaaaacctct cgccgcaacc ttttccattt cttcgccaat gcgctccaga agctttcggc
     4261 tgacctgagg atccaaagtg agataggttc cttctgtaga cttctgtatc gcttcaacaa
     4321 gcgtttgctc cagccggggt tccaacgtca tcacgtacag attcccatca ggagccagaa
     4381 atgactgcgt cagcggcctg gccaacgccg tgcgcaccag ttcggtcaac tgcgcaatat
     4441 cccgggtggc atgggcggcg tccgcaagtg cttcaagcac ggttcccaga tcacgaatgc
     4501 acacacgctc acgcagcaac atctgcagga ctttctgaat ctgccccacc gtcagcaggt
     4561 cgggaaccag ttcgttgacg aggacggtat gtgtttcccg cagccgatcg atcaggcgtt
     4621 gcacggcttg tctgtccagc agatcggggg catggg
//
LOCUS       DAOQUC010000112         4640 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_20651_length_4640_cov_3.075682, whole genome shotgun sequence.
ACCESSION   DAOQUC010000112 DAOQUC010000000
VERSION     DAOQUC010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4640
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20651_length_4640_cov_3.075682"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1404)
                     /gene="pyk"
                     /locus_tag="PKH07_03015"
     CDS             complement(<1..1404)
                     /gene="pyk"
                     /locus_tag="PKH07_03015"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HNT33949.1"
                     /translation="MNNATARTTIVATVGPASSDPQMLEQLVCAGVNVFRLNFSHGKN
                     EEHLERLNAIRAIAARHEKSVAVLQDLPGPKIRLGQFRNGEALLEPGTRFVLDNDPTP
                     GDAKRCAITYSELWKDVQKGQRILLADGVRELRVLSMEEDRVETEVVVGGVISDRKGV
                     NLPETRLRIPALTEKDRESLLWGIEHGVDYMALSFVRTAEDLQPAREALQAANSRTPL
                     IAKIEKPEAIRNLDSILDAADGVMVARGDLGVEMVPEKVPALQKEIILKANEKDKIVI
                     TATQMLESMIENPRPTRAEASDVANAIYDGTDAIMLSGETAVGKYPVQAVEMMRRIAL
                     ETEGHRFQCSAGRKPLSFETESLEKLVSHMASEASRNTNTRAICAFTCSGFTARLIAK
                     FRPPDPIYALTPDETTLRQMALVWGVQPILVPEVEDFEGVLKIVNETFCKRLGFQPGD
                     VVVVVCGLPVPVRGTTNT"
     gene            complement(1625..2080)
                     /locus_tag="PKH07_03020"
     CDS             complement(1625..2080)
                     /locus_tag="PKH07_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007795883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1810 domain-containing protein"
                     /protein_id="HNT33950.1"
                     /translation="MAQDESRDQDSHNLERFVSAQNGIFERVLTELRGGQKTTHWMWF
                     IFPQYKGLGSSSTSRHYAIKSLSEATAYLEHPVLGGRLLECCQALLQLECKSAHEIFG
                     YPDELKLRSCMTLFESISPSNSIFSEVLQKYFGGQKDQYTLTLISTDDH"
     gene            complement(2097..2996)
                     /locus_tag="PKH07_03025"
     CDS             complement(2097..2996)
                     /locus_tag="PKH07_03025"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNT33951.1"
                     /translation="MNNTPASSNTTVRLHPHRWVAELWLVVMTVFWGSSFTAIKTGLE
                     GTSPTGLVAYRFTIATAVFLLLAPGVLKELRTVGFRYGLFLAVILYFGYVLQTMGLEM
                     TTASRSAFVTSLHAAFVPLIWLCIARKIPQRNTLAGLGIAMVGLYFLLVKPEETQNLH
                     LNRGDVLTVFSAFAWAVYIVTLPILSKKSQFRPLMFGQLFGMAVLCWLVAGFQGSFPM
                     VDTGKSFFMILYLATMCTLVTVFLQTRYQQYTLAPRAAVIYTLEPVCAACIAFLFLSE
                     RMTGREMFGAALIFGGVLFSELA"
     gene            3051..3938
                     /gene="mazG"
                     /locus_tag="PKH07_03030"
     CDS             3051..3938
                     /gene="mazG"
                     /locus_tag="PKH07_03030"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HNT33952.1"
                     /translation="MQSFRGNQFAFPSAIWYRLFMVSKNTENNTNSNPPEELSVGQLF
                     EELCQKMAQLRSPQGCPWDREQTHLTLKKYLIEEAYEACEAFEDGDPKEIAEELGDVL
                     LQVVFHAEIGTQAGTFGVADVIRTLIDKLVQRHPHVFGDVPLETAQEVLQQWEENKLK
                     QSDRKESILDGIPKNLPALLKAMRVQERVQRVGFDWNQVAEVLAKVREEISEFEEALQ
                     QDNREKMQEELGDAFFALVNLARFVHLDPELSLARTVEKFCRRFRYIEQEVARKGLTL
                     RDVDLAEMDYHWEQAKTKV"
     gene            4038..>4640
                     /locus_tag="PKH07_03035"
     CDS             4038..>4640
                     /locus_tag="PKH07_03035"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HNT33953.1"
                     /translation="MKLSSSSWSFHRTFGAGQMDQFSWLDFCAGKLRLDGVELLDFHF
                     PNTEPSYLKDIKKYATDRGLTICCASVSNHFTGHYQDAQNDVEKVKKWIDIAQVLGAP
                     VVRVFAGSGEEMSKQVIYDQAVECLQKVAMYAEHVGVVLGLENHGGTTAEQVLALLRD
                     VQSPWLKLTLDTGNFPPSETYKSIEACLPHAVIVHAKLYEL"
BASE COUNT         1099 a         1166 c         1282 g         1093 t
ORIGIN      
        1 ggtgtttgtc gttccacgca ctggaacggg aagcccgcag acaacgacaa cgacgtcgcc
       61 aggctggaag ccaaggcgtt tgcagaacgt ctcgttgacg atcttgagga ctccctcgaa
      121 gtcttcgact tcaggaacca gaatcggttg taccccccag accaaagcca tctgtcggag
      181 tgttgtttca tctggagtga gggcatagat tggatcgggc ggtcggaact tggcgatcag
      241 ccgtgccgtg aaaccggaac acgtgaaagc gcatattgcc cgtgtgtttg tgtttctgga
      301 agcctcggat gccatgtggc tgacgagttt ttccagcgat tccgtctcga aactcagcgg
      361 tttgcggcct gcactgcatt ggaaccgatg gccttccgtc tcgagcgcga tgcgtctcat
      421 catttccacg gcttggacag ggtacttccc tacagccgtc tcgcctgaga gcatgatggc
      481 gtcagtgccg tcgtagatgg cattggcgac gtcagaagct tccgcacggg taggtctcgg
      541 attctcgatc atggactcga gcatctgcgt tgccgtgatg acaatcttat ccttctcgtt
      601 ggccttaagg ataatctctt tttgaagtgc gggaaccttc tcgggcacca tctcgacacc
      661 cagatcccca cgtgcgacca tgactccgtc ggccgcgtcg agaatgctgt caagatttcg
      721 gatggcttcg ggcttctcta ttttggcgat caggggagtt ctgctgttgg cggcctggag
      781 tgcctcacgc gcgggttgaa gatcttctgc cgtccgaacg aacgaaagcg ccatatagtc
      841 cacgccgtgc tcaatgcccc agaggagact ttcgcgatcc ttctcggtca gtgcgggaat
      901 cctgagacgg gtttcgggaa ggttcactcc ctttcggtcg gatatgactc ccccgacgac
      961 aacttccgtt tcgacgcggt cttcttccat ggaaagcaca cggagttccc tcaccccatc
     1021 cgcaagaagg atacgttggc ctttctggac atctttccac aactccgaat aagtaattgc
     1081 acagcgtttg gcatctcctg gagttgggtc attgtccagg acgaatcgag tccctggttc
     1141 cagcagtgcc tcgccattcc ggaactgtcc cagtcgaatc ttaggtccgg gcagatcttg
     1201 gagcacggct accgacttct cgtgtctggc ggcaatggca cgaatggcgt tcaggcgttc
     1261 caggtgttcc tcattcttgc catgagaaaa attgagtcga aagacattta caccggcaca
     1321 aacaagttgt tccaacatct gaggatcgga cgatgcgggt cctacggtgg cgacgatcgt
     1381 ggttcgggca gttgcattgt tcatccgtgg ctttcctcca gcggaacata ctagcaagac
     1441 cactccgaaa agcaactgct ttgggatttc tccaagggat tgggcatagc ccgcacaccc
     1501 agaactgctg actcgctctg tggactttgt gtgcatggcg tttcgaaacg ctgttcgatc
     1561 tgaatcaggc ccgggtgtat cagtctgtca atgttctgag atgggtcttc aggtagagca
     1621 aggatcaatg gtcatcggtg ctgatgagtg tcagtgtgta ttgatctttc tggccaccga
     1681 agtacttctg gagcacttcc gagaagatgg aattcgatgg agaaatgctt tcaaagagcg
     1741 tcatacatga tctgagtttc aattcatcgg ggtatccgaa aatctcatga gcggatttgc
     1801 actcaagttg cagaagtgcc tgacagcatt cgagcaacct tccgccaagt accggatgct
     1861 caaggtacgc tgttgcttcg gaaagactct tgatggcgta gtgcctggac gtagaactgc
     1921 ttcccagtcc tttgtattga ggaaagatga accacatcca gtgggtggtc ttctgaccgc
     1981 ctcgaagttc agtcaagact ctctcaaaga tgccattctg tgcggagacg aatcgctcca
     2041 gattgtgaga atcctgatct cttgattcat cctgcgccat aatctgactg ctcctctcat
     2101 gccaactcgg agaaaagaac acctccgaag atcaaggcag caccaaacat ctcgcgccct
     2161 gtcattctct cagaaagaaa caagaatgca atgcaggcgg cacagaccgg ttccaaggta
     2221 tagatgaccg ccgcacgagg tgccagggtg tactgctgat atcgggtctg aagaaatacg
     2281 gtgaccagcg tacacatggt ggcaaggtag aggatcatga agaaagactt tccagtgtca
     2341 accatgggga aagagccttg aaatcccgcg accagccaac acaggactgc cattccaaag
     2401 agttgcccaa acatcagggg tcgaaactgg gacttcttcg aaagaatcgg cagtgtcacg
     2461 atatacactg cccacgcaaa tgcagagaag acggtcaaca cgtctcctcg gttcagatga
     2521 aggttctggg tttcctcagg tttgacgagg agaaagtaca gaccgaccat ggcaattccc
     2581 aagccggcca gggtattgcg ttgtggtatc tttctggcga tacagagcca aatcaaaggg
     2641 acaaatgcgg cgtgaaggct ggtcacaaat gccgagcgag atgccgttgt catctccagc
     2701 cccatcgtct ggagcacata gccgaaatag aggatgactg ccagaaacag gccgtagcga
     2761 aaaccgaccg tccggagttc cttcagaacg cctggtgcaa gaaggaggaa gactgcagtg
     2821 gcaatggtga aacggtaggc aaccagaccc gtcggagatg ttccttccaa tccggtcttg
     2881 atcgccgtaa aggatgaacc ccagaaaact gtcatgacca ccagccacaa ctccgccacc
     2941 caccgatgtg gatgaaggcg caccgtcgtg ttgctgctgg ctggtgtgtt gttcataccg
     3001 ttctgccttg ctccttgcgt cgaggcactg aataatcgga gaccatagca atgcaatcgt
     3061 ttcgtggcaa ccagtttgcc tttcccagcg caatctggta tcgtctcttc atggtaagca
     3121 agaacaccga aaacaacacc aattcgaacc ctcctgagga actgtctgtt gggcagcttt
     3181 tcgaggaact ctgtcagaag atggctcaac ttcgcagtcc acagggctgt ccttgggatc
     3241 gagaacagac acacctgact ctgaaaaagt atctcatcga ggaagcatac gaggcttgtg
     3301 aagcctttga ggatggcgat ccgaaggaga ttgccgagga gctcggagat gtcctgcttc
     3361 aggttgtctt ccatgctgaa atcggcaccc aggcaggaac cttcggtgtt gctgatgtca
     3421 ttcggacgct cattgacaaa cttgttcagc gccatcccca tgtctttggt gatgtgcctt
     3481 tggagacggc gcaggaagtc ctgcagcaat gggaagagaa caagctgaag cagagtgatc
     3541 gcaaggagtc cattctggac ggcattccga agaatcttcc tgctttgctg aaggcgatgc
     3601 gggtacagga acgcgttcag agggtcgggt ttgactggaa tcaggttgct gaagttctgg
     3661 caaaggttcg agaggagatc tctgaattcg aagaagcact tcaacaggac aaccgtgaaa
     3721 agatgcagga agaacttgga gacgccttct ttgcactggt caatctggcg cgatttgttc
     3781 atctcgatcc tgaactgtcc ctggcgcgta ctgtggagaa attctgtagg cgatttcgct
     3841 acatcgagca ggaagtcgca cgcaaagggc tgacacttcg tgatgttgat ttagcggaaa
     3901 tggactatca ttgggagcag gcaaagacaa aagtctagtg ttttgtcacc ggcaggagta
     3961 ggaaccaaca gagtcgggtc agggtattac ctggcttaga tcttttggat tcagattcat
     4021 tttggggagg tagagccatg aaactatcga gcagcagttg gtcttttcat cgcacttttg
     4081 gggcaggcca gatggatcag ttctcctggc ttgatttttg tgccgggaaa ctccggctgg
     4141 atggagtgga acttctggat ttccattttc cgaacacaga gccgtcctat ctgaaggata
     4201 tcaagaaata cgccacagac cgaggcttga ccatttgctg tgcttccgta tcgaatcact
     4261 tcactggaca ctatcaggat gcacagaacg acgtggagaa ggtcaagaag tggatagaca
     4321 tcgcccaggt tttgggtgct cccgttgttc gagtgttcgc gggcagtggc gaagagatgt
     4381 ccaaacaggt gatctacgat caggcggtgg aatgtctcca gaaggtggcc atgtatgcgg
     4441 aacatgtggg agtggttctg ggtttggaga accacggtgg caccactgca gaacaggtgc
     4501 ttgcacttct cagagacgtg cagtctccct ggttgaaact cacgctggac acggggaatt
     4561 ttccgccatc ggagacatac aaatccatcg aagcctgtct gcctcacgca gttattgtcc
     4621 atgccaagtt gtatgaactg
//
LOCUS       DAOQUC010000113         4631 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_20696_length_4631_cov_3.481643, whole genome shotgun sequence.
ACCESSION   DAOQUC010000113 DAOQUC010000000
VERSION     DAOQUC010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4631)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4631)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4631
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20696_length_4631_cov_3.481643"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..387)
                     /locus_tag="PKH07_03040"
     CDS             complement(<1..387)
                     /locus_tag="PKH07_03040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33954.1"
                     /translation="MQKQDFLRFILFILIVFAVNSYFLGKKTEQHTAQQQEQPISREV
                     PQLASSPVQEASPEHLADVTEPIRMETPPTVESVSKEPVFFVTGPEKTVRVRSNLYDV
                     TLHTRGARATSWKLIQWAEMPFADSRL"
     gene            complement(454..666)
                     /gene="yidD"
                     /locus_tag="PKH07_03045"
     CDS             complement(454..666)
                     /gene="yidD"
                     /locus_tag="PKH07_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HNT33955.1"
                     /translation="MVAKVLLMLLRIYQRWISPLYPARCRYVPTCSQYAVEAIKKYGA
                     LRGTWMAVWRVLRCHPWSRGGYDPVS"
     gene            complement(688..1050)
                     /gene="rnpA"
                     /locus_tag="PKH07_03050"
     CDS             complement(688..1050)
                     /gene="rnpA"
                     /locus_tag="PKH07_03050"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HNT33956.1"
                     /translation="MTLRRQTLRRYEKIRKKAQYESIFALGQVVRSRAFDLFYDQVGQ
                     GRRQAGFVVSRKVATRATTRGRIKRRMREIFRRNKDRLAEGTHIIFRANPGVEELNFE
                     QLENTFFQLSERIRSRTR"
     gene            complement(1047..1184)
                     /gene="rpmH"
                     /locus_tag="PKH07_03055"
     CDS             complement(1047..1184)
                     /gene="rpmH"
                     /locus_tag="PKH07_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006461360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HNT33957.1"
                     /translation="MKRTYQPSRIRYKRTHGFRSRMSTRGGRLVLKRRRQKGRKRLSA
                     L"
     gene            complement(1410..2234)
                     /locus_tag="PKH07_03060"
     CDS             complement(1410..2234)
                     /locus_tag="PKH07_03060"
                     /inference="COORDINATES: protein motif:HMM:NF012529.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNT33958.1"
                     /translation="MPTHTKQTVETVVKKIDERRKHPKGKPRKFPYGCDQCDGYGNIV
                     TEQGARACECKKRYFIQVHLEQANIPPIFEDKSFDTFENPTPEHEKDLKWAREYVKNY
                     SSTNKRGVLFIGHPGVGKTHLAISILRELILKGYTGLFFNMNDLLQEIRATFDENSSA
                     TEFDILERLLGVDVLVLDDVGAQERISGFVLDRFYTLVNGRYQANKTLIVTTNSDPKT
                     LEQRLQAHTISRLYEICELRMASVETDYRTSNYAGSPGPKKRGARGVRRAGHPEGL"
     gene            complement(2206..2844)
                     /locus_tag="PKH07_03065"
     CDS             complement(2206..2844)
                     /locus_tag="PKH07_03065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33959.1"
                     /translation="MRDDLLYTCSPRWLEESGLPEEIVERVGEAGWSVLKKLFELETE
                     QNFFPDWFLVSREDLHRWTGALSDRIDEVIKCLEQEGYLERKEGVTPGGLEHFRFLEP
                     LLTEEEAERTRERLKARGYTGVTHLTLRYVDAMPAKDKFRTVRQLYEQVFGLRMNTRI
                     ADDLREITERFDLALILSAFEEMRRKPNKSLAGLITALYRGERHADTHQADR"
     gene            complement(2897..3697)
                     /gene="cysE"
                     /locus_tag="PKH07_03070"
     CDS             complement(2897..3697)
                     /gene="cysE"
                     /locus_tag="PKH07_03070"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HNT33960.1"
                     /translation="MALLVVQWVLVAIGALVLLAFVINIPKIWSDYWCVFQRDPAARG
                     FWGYLEVLLTYAGFHAIAAYRVAHFLLKMRIPFVPRALSQLARLITGVEIHPGALIGK
                     GFFIDHGMGVVIGETTIVGENCTLFQGVTLGGTGKESGKRHPTLGRNVMVSTGAKILG
                     NIEIGDNAKIGAGSVVLKSVPADCTVVGIPGKIVRRKGERIGSPDLDHSDLPDPIAER
                     LRAMQREIECLAEKIHCYDPDCKIRQDATRFDREPIEPGKENQAADEA"
     gene            3912..>4631
                     /locus_tag="PKH07_03075"
     CDS             3912..>4631
                     /locus_tag="PKH07_03075"
                     /inference="COORDINATES: protein motif:HMM:NF024956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HNT33961.1"
                     /translation="MNEDGSTTAFPFRRPAFLACLAFLGGLVCGRAWDPSATLLLSGC
                     LFLAVLLCAAFRYGVRDSFPALLPLFLLLTFLLGIMRFEWHQDNWRDFQNETSLYSRQ
                     GTQTLHGTVNGAVEGTGSYSQTSFPIKDVSIVVEKQEYPVGSSILVTAYCPLEATPQF
                     GDVIQCVGLLEPIRAVALPGQVDNKAYLASEGIHGRVRIYEPSQLRLIRAEHKSPYVH
                     MRRAIFSLRQAILKTYRQRLPA"
BASE COUNT         1045 a         1313 c         1146 g         1127 t
ORIGIN      
        1 caggcgtgaa tcagcaaacg gcatctccgc ccactgaatc aacttccacg aggtggcacg
       61 tgctccacgt gtatgcaatg tgacatcgta gagatttgat ctgacacgaa cggttttctc
      121 cgggcctgtg acgaagaaaa caggttcctt ggagaccgac tcaacagtgg gaggagtctc
      181 cattcgtatc ggctctgtga catcagcgag gtgttcgggc gacgcttcct gaacgggaga
      241 cgatgccaat tgaggcactt cgcgtgaaat gggttgttcc tgctgttgag cggtgtgctg
      301 ttcggtcttc tttcccagaa aataggaatt gaccgcaaac acgatcaaaa tgaacaggat
      361 aaacctcaga aagtcctgtt tctgcataaa tcctttctac gggcttctgt gcaagacgac
      421 agacagaaga aaaaacaacg ataccggact ccgtcaggat accggatcgt acccacctcg
      481 actccaggga tgacagcgca gaacacgcca gacagccatc caagtaccgc gtagggcgcc
      541 gtacttcttg atggcctcaa cggcatactg cgaacaagtc ggcacataac ggcatcgtgc
      601 cggatacaga ggtgaaatcc accgctggta tatcctcaaa agcatgagca gcacttttgc
      661 gaccatgctg ccaactgcct ttcctgttca tcgagtgcgg cttctgatcc gttcggacag
      721 ttggaagaag gtattctcca gttgttcgaa gttgagttct tccacacccg ggttggctct
      781 gaatatgata tgcgtgcctt cagccaacct gtccttatta cggcgaaaga tctctctcat
      841 tcgccgctta atccgtcctc gtgttgtagc ccgggtggcg actttgcgac tcaccacaaa
      901 gcccgcctgt cgtcggccct gccccacctg atcataaaat agatcaaagg cccgggaacg
      961 aaccacttga ccaagcgcaa aaatactctc gtattgggcc tttttcctaa tcttctcata
     1021 gcgacgtaac gtctgtcgcc ggagtgtcat agcgcgctca gtctctttct tcctttttgg
     1081 cgacggcgct tcaggacaag tcggccgcct cgggtgctca ttcggctgcg aaatccgtgc
     1141 gtccttttgt acctgatgcg actcggctga taagtcctct tcaaaagtcc tcctcactcc
     1201 tttctctcca agtgtgttgc cggtctggcg ccagcgcaca gagacaacac tattccctcg
     1261 cggaaaaccc gcgtcaggct caaaacttgg gggattatag agaacagccc caaactgtca
     1321 agcaaaacag ggtgcggtcg gaaattcaca gatcctttga gaaaaaggca gggaatcctc
     1381 tgacaacgcc cgttgctcta actgaagacc tatagtcctt cgggatgtcc cgcccttcgg
     1441 acaccacgcg ccccgcgctt tttggggccg ggagagccgg catagttgga tgttcggtag
     1501 tcggtttcca cggatgccat ccggagttca cagatttcgt acagccgcga aattgtatga
     1561 gcttgcagac gttgctccag agtcttggga tccgagttgg tcgtaacaat gagtgtcttg
     1621 ttggcctgat agcgtccgtt gaccagagtg tagaaacggt cgagcacaaa accggaaatg
     1681 cgttcctgcg ctcccacgtc atcgagaacc aggacatcta ctccaagaag cctttccaga
     1741 atgtcaaact ctgttgccga actgttctcg tcaaaagttg cgcggatctc ctgaagcaga
     1801 tcgttcatgt tgaaaaaaag gccggtgtat cctttcagga tcaactcgcg caaaatcgaa
     1861 atggccagat gtgtctttcc cacacccgga tggccgatga acagaacacc cctcttgttg
     1921 gtactggagt aattcttgac gtactcacgc gcccacttca gatctttttc gtgttcgggt
     1981 gtcgggtttt caaaagtgtc gaagctcttg tcttcgaaaa ttggggggat gttggcctgc
     2041 tccaagtgaa cctggatgaa atagcgcttc ttgcattcgc aggcgcgtgc cccttgctcc
     2101 gtgacgatat tgccatatcc gtcgcattgg tcgcagccgt aaggaaactt tctgggcttc
     2161 ccttttggat gctttctccg ttcgtcaatc ttcttgacga cggtctcaac ggtctgcttg
     2221 gtgtgtgtcg gcatgtcgtt ctcctcggta cagcgccgta atgagccctg caagcgactt
     2281 gtttggtttt ctccgcatct cttcaaacgc actcagaata agagccagat caaatcgttc
     2341 ggtgatttcc cgcaggtcgt cggcaatacg tgtgttcatc cgaagaccga aaacctgttc
     2401 gtacagctga cgcaccgtac gaaacttgtc cttcgctggc atggcatcca catagcgcag
     2461 agtcagatgc gtcacaccag tataacccct ggctttcagc cgctcccggg ttcgttccgc
     2521 ttcctcttcg gtcaagagcg gttccaggaa tcgaaaatgc tccaaacctc ccggcgtgac
     2581 gccttccttt cgttcaagat agccttcttg ctcgagacat tttatcacct cgtcaatacg
     2641 atcggacaac gctcctgtcc agcgatgcaa atcctctcgg ctgaccagaa accagtcagg
     2701 gaagaagttt tgctctgtct ccagttcaaa gagcttcttc agaacggacc aacccgcctc
     2761 tcccacacgc tcgacgattt cttccggcaa gccgctctcc tcgagccacc tgggggaaca
     2821 ggtgtaaagc aggtcatcac gcattggatc gtgcttatcc tttcagcagt ccccacatct
     2881 cgttcgtgca ggcagatcag gcctcgtccg cagcctgatt ctccttgcca ggctcaatgg
     2941 gctctcggtc aaatcgggtg gcatcctgac ggattttaca gtcgggatca taacagtgga
     3001 ttttctcagc cagacattcg atctcccgct gcatcgcccg aagccgttcg gcgattgggt
     3061 ccggtaagtc cgaatgatcc agatcgggag aaccgatgcg ttctcctttg cgccgaacaa
     3121 tctttcccgg aattcccacg acggtacagt cggctggaac agacttcagg acgaccgatc
     3181 ccgctccgat cttggcgttg tcgccaatct cgatatttcc aagaatctta gctccggtgg
     3241 aaaccatgac attcctgccc agagtcgggt gccttttccc agactccttg cctgtaccac
     3301 ccagcgtcac tccttgaaac aaagtgcagt tttctccgac gatggtggtt tctcctataa
     3361 caacacccat gccatggtca atgaagaagc ccttcccgat cagagcgccg ggatgaatct
     3421 ctacgcccgt tatgagtctc gccaactgcg acagtgctcg tgggacaaac ggaatccgca
     3481 tcttcaacag aaaatgcgcc acgcggtaag cggcgatggc gtggaaaccc gcgtaggtca
     3541 gcagcacttc caggtatccc cagaaaccac gggcagctgg atctctctgg aaaacacacc
     3601 agtagtctga ccagattttc ggaatgttga tgacaaaagc caaaagcacc aacgcgccga
     3661 tggcaacaag tacccactga acgacaagca aggccatgac aaccactcct tcagacaagt
     3721 cacacgagtc ggcatgacag gaaaacccat cacccactca ctttctgtga ctctatcaca
     3781 atctccgctc tgacaaaagc aggacatcag catttgatcc ccgtatcgtt tccgactatt
     3841 gttctctgta ttcgttgggt ttgaccgcat catcaatata tgaggcaaca gcggatctga
     3901 acttgggcgt catgaacgaa gacggttcga caacagcatt tccatttcga agaccggcct
     3961 ttctggcttg ccttgctttt ctgggcggac tggtctgtgg tcgcgcttgg gatccttccg
     4021 ccacgctgct tctgtcgggg tgtctgttcc tggcggtact tctctgcgcg gcgtttcggt
     4081 atggagtaag ggattcgttt cccgcactct tgcctctgtt ccttctcctg acattccttt
     4141 tgggaatcat gcgctttgaa tggcatcagg acaattggag ggacttccag aacgagactt
     4201 ctctctattc aaggcaaggc actcaaacgc tccatggaac agtcaacggt gcggttgagg
     4261 gaaccggcag ctactcacag acttcctttc ccatcaaaga tgtttccatc gttgtggaga
     4321 agcaggagta cccagttggt tcaagcattc tggtcaccgc gtattgtcct ctggaggcta
     4381 cgccccagtt cggagatgtg attcagtgtg tgggactgct cgaacccatt cgtgccgtgg
     4441 ctctgccggg acaggtggac aacaaagcct atctggcttc cgagggaata catggccgtg
     4501 ttcgaattta tgagccaagc cagctgaggc tgatccgagc tgagcacaag agtccctatg
     4561 tccatatgcg aagagcgatc ttttctcttc gccaagcgat tctgaaaacg tatcggcaac
     4621 gacttcccgc t
//
LOCUS       DAOQUC010000114         4625 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_20741_length_4625_cov_2.680306, whole genome shotgun sequence.
ACCESSION   DAOQUC010000114 DAOQUC010000000
VERSION     DAOQUC010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4625)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4625)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4625
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20741_length_4625_cov_2.680306"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..540
                     /locus_tag="PKH07_03080"
     CDS             <1..540
                     /locus_tag="PKH07_03080"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNT33962.1"
                     /translation="AIAFLMSCWTNDVVTRSLRGSGDPEVDVLLQLSGEETDPAAVDA
                     SNARLTWIISLSLLVCVAGVLNSMLMAVTERFQEIGTMKCLGALDPFIVKIFLLESLL
                     LGSIGSALGLLVGLALSLIGLSVGFGKYVWQYFPMVQLMHYALYSFLIGTGLSVLGAI
                     YPAWTAAQMEPVEAMRVER"
     gene            569..1306
                     /locus_tag="PKH07_03085"
     CDS             569..1306
                     /locus_tag="PKH07_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013513555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNT33963.1"
                     /translation="MAEQIIVRANSVKKTYQMGKFKLDALRGVNLEVNQGEYLSIMGP
                     SGSGKSTLFNMIGGLSKPSEGTVYIDEVDIAQLDAYELAWLRCRKIGYIFQTFNLIPV
                     MTALENVTLPMTFAGMSDDEARDKGVELLSRVGLGDRVQHKPFELSGGQQQRVAIARS
                     MANDPAIILADEPTGNLDLRTGKEIIELLKKLQTSSGVTIISATHDMKMLSVSDRVVW
                     IRDGMIDRIENREDLDIQVGTIDGAQE"
     gene            1606..1683
                     /locus_tag="PKH07_03090"
     tRNA            1606..1683
                     /locus_tag="PKH07_03090"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1641..1643,aa:Val,seq:gac)
     gene            1827..3806
                     /gene="thrS"
                     /locus_tag="PKH07_03095"
     CDS             1827..3806
                     /gene="thrS"
                     /locus_tag="PKH07_03095"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014453793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HNT33964.1"
                     /translation="MVEVQLADGRKLTMQAPATGEQAWGSLVKGDSMPVAYKQCGRLF
                     DMSRPIEEGSAVEFVSADSEEGREVLRHTTAHVMAQAVCHLYPKTKLAIGPAISDGFY
                     YDFDTDKTFSSEDLQAISDEMSRIATQNTPLRRVELGRQEALEHFRQKNQPYKVEMLE
                     QMTDETVSLYRQDDFEDLCRGPHLLSTGCLGAFKLLEVAGAYWRGDERNPMLQRIYGT
                     AFETQEALEQHLAKLQEAKERDHRKLGQQLDLFSSQEMIGAGLVLWHPNGGRLRTTIE
                     DFWRQEHYRRGYELVYTPHIAKSDVWKTSGHLDFYREGMYSPMDIDGQDYYIKPMNCV
                     GHILIYKSRLRSYRDLPIRLGELGTVYRYERSGALQGLLRVRGFTQDDAHLFCTPEQL
                     LDEIVGVVNFVDFMMLERFGFKYRLYLSTRPEHSVGTDEDWARATEALKSALQKLGKT
                     YEIDPGEGVFYGPKIDVKLIDALGREWQGPTIQVDFNEPERFDVTYVDKDGQRRRVVM
                     IHRVVLGSMERFIGCLIEHYKGDFPLWLAPVQSVVLPVTDAVNDYAVLVKEHLRSVGL
                     RVETDLRSEKIGQKIRDAELQKIPCMLVVGKREREANSVSLRRRKQGDLGSLTLDALR
                     ESMQREVDNPTVENQSEKGGESSLEDRSFQGAVSR"
     gene            3806..4342
                     /gene="infC"
                     /locus_tag="PKH07_03100"
     CDS             3806..4342
                     /gene="infC"
                     /locus_tag="PKH07_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020887314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HNT33965.1"
                     /translation="MQARINELIRAKEVRLVDDTGDHQGIVPIQEALRRAQEAGLDLV
                     EVAEKAEPPVCRIMDYGHYRFEQAKKEKDARKRQRTITVKKIEMGLNISEHDYQFKLR
                     HAERFLQHGDKVKVVAKFRGRQITFKDQGKDLLERTIEDLKKFGTPEQDARMDGRMMT
                     VVIAPIPSANKPPVSTQE"
     gene            4438..>4625
                     /gene="rpmI"
                     /locus_tag="PKH07_03105"
     CDS             4438..>4625
                     /gene="rpmI"
                     /locus_tag="PKH07_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HNT33966.1"
                     /translation="MPKIKTNRSAAKRFKLTKGGKVKRAKAYKSHILTSKSTKRKRKL
                     RQGTLVCKADEHRVKKMLG"
BASE COUNT         1074 a         1098 c         1395 g         1058 t
ORIGIN      
        1 gccatcgcct ttctgatgtc gtgctggacg aacgacgtgg tcacccgcag cctgagaggt
       61 tcgggtgatc cggaagtgga tgtgctcctg caactcagtg gcgaggagac cgatcctgcg
      121 gcggtggatg ccagtaatgc caggttgaca tggatcatct cgctctcgct tctggtttgt
      181 gtggcgggag ttctcaactc gatgcttatg gcggtcacgg agcggttcca ggaaatcggg
      241 acaatgaaat gcttgggagc gttggatccc ttcatcgtca agatattcct tttggagtcg
      301 cttttgctgg gaagtattgg atccgcgttg gggctgttgg tcggcttggc tctgtcgctg
      361 atcggtttga gtgttggctt tggcaaatat gtatggcagt acttccccat ggtgcagttg
      421 atgcactatg ctttgtactc gttcctcatt ggtacagggc tgtcggtttt gggggcgatc
      481 tatcccgcct ggacagcggc acagatggag ccggtcgagg caatgcgtgt cgaacgatag
      541 gaaatctgga agagaaaggt ttgggaacat ggctgaacag atcatcgtcc gggcaaacag
      601 tgtcaagaag acctaccaga tgggcaagtt caaactcgat gctctccgtg gggtcaacct
      661 ggaggtcaac cagggagagt atctctccat catggggcct tccggctccg ggaagtctac
      721 tttgttcaac atgatcgggg gcttgagcaa gcccagtgag ggaacggtct atattgatga
      781 agtggacatt gctcaactgg atgcctatga actggcgtgg cttcgatgcc ggaaaatcgg
      841 ttatatcttc cagacgttca atctgattcc cgttatgacg gcgctggaaa acgtcacgct
      901 gcccatgacg tttgccggca tgagtgatga cgaggcgcgt gacaagggtg tggaactgct
      961 gagccgggtt ggcttgggag atcgggttca acacaaaccc tttgaactct ccggtggaca
     1021 gcagcagcgt gttgccattg cccgatccat ggccaatgat cctgccatca ttctggcgga
     1081 tgaacccacc ggaaacctcg atctgagaac aggcaaggag atcatcgaac tgctcaagaa
     1141 actgcagaca tcctcgggtg tcacgatcat ctctgctacc catgatatga agatgctcag
     1201 tgtgtccgat cgagttgtct ggattcggga cggtatgatt gaccgcattg agaaccgcga
     1261 agatctggac attcaggtgg gtaccattga cggtgcccag gaatagtctg ggacgatttt
     1321 tcgcgaacga gagggatttg cgataagccc attttgggcg gcttgtcatt ctgaacgaag
     1381 cgaagcggag tgaagaatct ctctgatagc ataagcctta tgacaggaag agcgattctt
     1441 cgctttgctc agaatgacca caacggtgca ttctgtcggc ctaccgcaaa tgcctccgaa
     1501 cgaggttttc gcttgacaac tgaattgtct ggccattagt cttctgcaac attgtgaaga
     1561 tggggccggc gtgctttttg agtgttaccg tgcccgcagc gtttcgggtc gttagctcag
     1621 tttggttaga gcgctacgtt gacatcgtag aggtcactgg ttcaagtcca gtacgaccca
     1681 ccatgataca cacgttcccg aagggggttg gagacgctcg gaatatgccc ctggtctggt
     1741 agagccagga gaagagtcac ggtagcgcca gacgacaaga gcgctatcgt gttttttttg
     1801 aacgcggaca gcgagagaga acgcagatgg ttgaagtcca attggcggat ggcagaaaac
     1861 tgacaatgca agcacctgcc acgggtgagc aggcctgggg ttcccttgtc aagggggatt
     1921 cgatgcccgt tgcctacaag cagtgcggcc gcttgtttga catgagccga cccattgagg
     1981 aaggaagtgc tgtggagttc gtttctgcag actctgagga aggtagagaa gtgcttcggc
     2041 acaccactgc ccacgtgatg gcacaggcgg tctgccatct ctatccgaag accaaacttg
     2101 ccatcgggcc agccatttcc gatggcttct actatgactt tgatacggac aagacttttt
     2161 catcggaaga ccttcaggcg atttccgatg agatgagcag aattgccact cagaatactc
     2221 cgcttcggcg tgttgaactc ggtcgtcaag aggcgctcga gcattttcgc cagaaaaatc
     2281 aaccctacaa agttgagatg cttgagcaga tgaccgatga gacggtctca ctctatcgcc
     2341 aggatgattt tgaggatctc tgtcgaggcc cacacctttt gagcacaggc tgtctcgggg
     2401 cgttcaaact gcttgaggtg gccggagcct actggcgcgg ggacgaacgc aaccccatgc
     2461 tgcagcgcat ttatggaacg gcgtttgaga ctcaggaagc gcttgagcag cacctggcga
     2521 aactacagga agccaaggaa cgcgatcacc gcaaactcgg tcagcaactg gatttgttca
     2581 gcagccagga gatgatcgga gcggggctgg ttctctggca tcccaatggc ggtcgcctgc
     2641 gcaccaccat cgaggacttc tggcggcagg aacattaccg ccgaggttat gaactggtct
     2701 atacgcccca catcgccaag agtgatgtct ggaaaacatc gggacacctg gatttttatc
     2761 gggaagggat gtactccccg atggacattg atggtcagga ttattacatc aagcccatga
     2821 actgcgtggg tcatattctg atctacaaga gccggcttcg cagttatcgg gatcttccca
     2881 ttcgcttggg tgagttgggc accgtctacc gctacgagcg ttccggcgcg ttgcaggggc
     2941 ttttgcgggt acgcggattc acgcaggatg acgcgcatct gttttgcaca ccggagcaac
     3001 tcctggatga gattgtcggt gtggtgaact ttgttgattt catgatgttg gagcgctttg
     3061 gcttcaagta tcgtctctat ctgtctaccc gtcccgagca ctcagtcggc actgatgaag
     3121 actgggcgcg ggcaacggaa gccctgaaga gtgcccttca aaaactcggc aagacgtatg
     3181 agatcgatcc cggggaaggg gttttctacg gtcccaagat tgatgtgaaa ctcattgatg
     3241 cgctgggtcg tgaatggcag ggaccgacga ttcaggtgga cttcaacgaa ccggagcgat
     3301 tcgacgtgac ctatgttgac aaagacggtc agcgtcggcg cgttgtgatg attcaccgtg
     3361 ttgtgctcgg ctccatggag cgtttcatcg gatgtctcat tgagcactac aagggtgatt
     3421 tccccctgtg gctggcaccg gtgcagtcgg ttgtgcttcc cgtgacggat gcagtcaatg
     3481 attatgccgt ccttgtcaag gagcatctgc gttcggtggg gttacgggtg gagaccgatc
     3541 ttcgttcaga gaaaatcggc cagaagatac gggatgccga attgcagaag attccgtgta
     3601 tgctcgttgt gggcaaacgg gaacgagaag ccaattccgt ttctcttcga cgccgcaaac
     3661 aaggcgacct tgggtcgctg acactggatg cgctgcggga atccatgcag cgcgaggtgg
     3721 acaatccaac ggtggagaat cagtccgaga agggagggga gagttcatta gaagaccgtt
     3781 cttttcaagg agccgtgagc cggtaattca ggcgcggatc aatgaactga ttcgtgccaa
     3841 ggaagtcaga ctcgttgacg acacgggcga tcatcaggga attgttccca ttcaggaagc
     3901 cctgaggcgt gcgcaggagg ccgggctgga tttggtcgag gtggccgaga aggctgagcc
     3961 tcccgtgtgc aggattatgg actacggtca ttaccgattc gagcaggcaa agaaagagaa
     4021 agacgcgcgc aagcggcaac gaaccattac ggtcaagaag atcgagatgg gtctgaacat
     4081 cagcgagcat gactatcagt tcaagttgcg tcatgccgag cgtttcctgc aacacggcga
     4141 caaagtcaag gtggttgcca agtttcgagg acggcagatc acgttcaagg atcagggaaa
     4201 ggatctgctg gagcgcacaa ttgaagactt gaagaagttt ggcaccccgg aacaagatgc
     4261 caggatggat ggccgaatga tgactgttgt cattgcgccc attccgagcg cgaacaagcc
     4321 acctgtctca acgcaggaat agttgtgagc gtcaagggtg ttgacaagga ctttcggtca
     4381 tgaggaaggc tctaagaggg ctttctctcg ttattttttg gataaaggag gcatgaaatg
     4441 ccgaagatca agaccaaccg gtctgccgcc aagcgcttca aactcaccaa gggtgggaaa
     4501 gtcaagcgcg ccaaggcgta caagagtcat atcttgactt cgaaatcaac caagcgaaag
     4561 agaaaacttc gccagggaac cttggtttgc aaggcagatg agcatcgggt caagaagatg
     4621 ttggg
//
LOCUS       DAOQUC010000115         4624 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_20747_length_4624_cov_3.235062, whole genome shotgun sequence.
ACCESSION   DAOQUC010000115 DAOQUC010000000
VERSION     DAOQUC010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4624)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4624)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4624
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20747_length_4624_cov_3.235062"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1711)
                     /locus_tag="PKH07_03110"
     CDS             complement(<1..1711)
                     /locus_tag="PKH07_03110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33967.1"
                     /translation="MNTLRVQTFRSFWLCLSVVFCAQQSHAALAKYKPNFCMIPTLKE
                     AWIVDGQLDDAVLEKAPRFSGLVRAGGACVAESQTEISLFSDGESLCVAFLCHEATPS
                     EMLAQYIARDSRVWDDDSVEVFIQCPWQQEDYFHFVLNTRNVICDEHIQAKEWNANIE
                     TETFVGSDFWSAEMRIPLHELGGAFHLDLPFKVNFVRNRQNPAVREMSTWSIVEKSNH
                     DIETFGAIMRAEKPIPGWQLRVPNRFYPGRTTIGVNPGLGGKVHAILGCWPCNGLLFI
                     SETLTQDSDITYILTPDTKGQTFEVREGNSKFGELLYRTQWPVKPPKYESLEPLKKLS
                     AKVSKDIQALPRDTQARFMMDQAKDTEELQNLVRIRSDEEKLTLDAWTELEQRAFDLE
                     VRLNNRNGSVQARKKSPKSSLVIGLCSSMLKVFIRDHAYPGQFTDHWSLQMARGEREA
                     FQVSVLATDEPLSGVQVFPGEFRCGTYRFPSENISVSLVGYVLTRQPSYEVDYVGWWP
                     DPLLTFQKTADARLGETLTFWVEFHAPESLAPGTYQGEIVLQTAKGDRQVVTVEVEVW
                     DLLI"
     gene            2053..>4624
                     /locus_tag="PKH07_03115"
     CDS             2053..>4624
                     /locus_tag="PKH07_03115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33968.1"
                     /translation="MNRTNDLVLVLWVCLMVSVPMTGLANQQESLSEMGAITLPFHEM
                     FEGSKIGEAWTAVVSEGNSLSIENNSLRIEATAHASAHLQRPLGEDLITVTAKIWGAA
                     FYLVWNAENGCGMGRVSPTPFPRFFISQTIQGTTRESHLVGCSISIPYYLRFQIASDC
                     FRFSYSEDDKEWRHLKTIPRPDSLAGAPSLMVFGNYYGEDDVLPFGDVAMSPDGDRGS
                     KYTCFVGEIRIEKTPEDALLMTSAEKRAIETEGLDPVAQVVLRGSEDPTYESVEAFYP
                     PFKFPREVVGVPEHPLDIGIDYQGRLDVSPWDRPYAWIEIGDPLKPFGDTPIARRLQK
                     GYLPIVTLSTERDDIRYEMAVFGWSEDFRVDKDLFAYVRLRARTSKSGQEVKPIALVD
                     ARSGARTEWPLLKIGRRDYEVCLSFKYPEPSSAQRVSSSAFERISRNVTAHWEKRLEI
                     AKRVRVPDQRMNEAYKAWIVYSLLNADTVNGYVEARDGSGFYDEMFGYSVSLHTMAAD
                     LFGMHNYAERCLQTQLHFQAEDGMYVQATGLPDLGSLLMALYEHYLVTRDVAWLQSVA
                     PNIDKGCQWILQQRQATPKEGITKGMIKFRPYCDYPVPVYNFMADIYACMGLERGAEA
                     LQVLGMRSEAERYAAEADAYRNDLLAAMDAAAFQDGDLKILPMEPETRRLLKLDQYQG
                     GGYYGLVCGILLETGFLTPDDVRSHWITDIMERRNGLIGGVCEFQLGVDHAYTYGYLM
                     TKMQRDNIRNVILGLWGMMAFGMTRDTYSPVEVNMIQTGDNHLTLPHLYSCTQQLRLL
                     RNMLLCEEGDVLWIGRAIPRVWLEPDKRVEVKSAPTHFGEVSYSIDAKRNGNMVVRIS
                     P"
BASE COUNT         1114 a         1221 c         1238 g         1051 t
ORIGIN      
        1 gaatgagaag atcccagacc tcgacctcta cggtcacgac ctgcctgtcc cccttggcgg
       61 tctgtaggac aatttctcct tgataggttc ctggcgcgag cgactcagga gcatgaaact
      121 ccacccagaa agtcagtgtc tcacccagtc gggcatcggc ggtcttctga aaggtcagga
      181 ggggatcagg ccaccaaccc acgtagtcca cttcatacga aggctgtcga gtcaagacat
      241 atccaacgag actgaccgaa atgttctctg agggaaaacg gtatgtcccg catcggaact
      301 cacctgggaa tacttgcact ccagacagag gctcatcggt ggcaagaacc gatacctgaa
      361 aagcttctcg ctccccacgt gccatctgca aagaccaatg atcagtgaac tgcccaggat
      421 aggcatggtc gcggatgaac accttcaaca tggatgaaca gagaccgatc accaaagagg
      481 atttgggaga tttctttctt gcctgcacgg agccgttccg attgttgaga cgcacttcca
      541 aatcgaaggc gcgttgttcg agttctgtcc aggcatcgag tgtgagcttc tcttcatccg
      601 aacgaatgcg cacaagattc tgcagctctt ccgtgtcttt ggcctgatcc atcatgaatc
      661 gggcttgcgt gtcccgaggc agagcctgaa tatccttgga gaccttcgcg ctcagtttct
      721 tgagtggctc cagggactca tacttcggcg gcttgacagg ccactgggta cggtagagga
      781 gttctccaaa cttgctgttc ccttctcgaa cctcaaacgt ctgtcccttt gtgtcaggag
      841 tcagaatata cgtaatgtcc gaatcctggg tcagcgtctc agaaatgaac agcagcccat
      901 tgcagggcca gcacccgaga atggcatgaa cctttccacc cagacctgga ttgacgccga
      961 tcgtggttct tcccggatag aagcgattgg gaacccgcaa ctgccagcca gggattggct
     1021 tttcggcacg catgatcgct ccaaaagtct caatgtcatg gttactcttt tccacgatag
     1081 accaagtgga catttcacga actgccggat tctgtcgatt gcgcacaaag ttgaccttga
     1141 atggcaagtc cagatgaaat gcgcccccga gctcatgcag cggaatccgc atctcggcgc
     1201 tccagaaatc actcccaacg aatgtttccg tctcgatgtt ggcgttccac tcttttgcct
     1261 gaatatgctc atcacagatg acattgcgcg tgttcaagac aaaatgaaaa tagtcttcct
     1321 gctgccaggg gcactggatg aacacctcaa cactgtcgtc atcccagacc cgactatccc
     1381 gagcaatgta ctgtgcaagc atctctgacg gcgttgcctc gtgacacaga aatgccacgc
     1441 agaggctctc tccgtccgag aagagagata tctccgtttg agactctgcc acacaggccc
     1501 cgcccgcccg aaccagaccg gaaaatcggg gagccttctc caacacagca tcatccaact
     1561 gaccatccac aatccacgct tctttcagtg ttggaatcat gcagaagttc ggtttgtatt
     1621 ttgccagggc ggcgtggctc tgctgggcac agaacaccac agacagacac aaccagaaac
     1681 tccgaaacgt ttgtacgcga agagtgttca tgcgattaga tcctccagat gcttgctcga
     1741 ctgaaagcaa tccgcggact gctccaatag ggcgttcaat gctctagcgg cagactgtcc
     1801 agtgaagaat tcatactcca gcacaaggct ctttccctga gacaggcagg agcaagaacg
     1861 cattcacata caagccagag cgtgacaagg tcagctgaag aatctcaaat cctgccgagg
     1921 agcgcaagcg agcaacgtgg tttctgccag aatttgagag tgaggatcga ttgctgcaac
     1981 acgctgttgc cagacaatct gcgcacaagt attcttggtg ttggccgact tttcattgga
     2041 ggaaaacatc tcatgaaccg aacaaacgat ctcgtccttg ttctttgggt gtgtctcatg
     2101 gtgtctgtac cgatgactgg actggctaat caacaggaat ccttatctga gatgggcgcc
     2161 atcaccctgc cttttcacga gatgttcgag ggttccaaaa tcggtgaagc ctggacagcg
     2221 gttgtctcag aaggcaatag cctgtccatc gaaaacaatt cgctacgcat cgaggcaact
     2281 gcgcatgcct cggctcatct gcaacggccg ctcggggaag acttgatcac cgtgacggcc
     2341 aaaatctggg gggcggcttt ctatctagtc tggaatgcgg agaacggttg cggaatgggg
     2401 cgagtctcgc caacaccctt tccgcggttt ttcatcagtc aaacgattca gggcacaacc
     2461 agagagtccc atctcgttgg atgctcgatc agcattcctt actacctgcg gtttcagatt
     2521 gcgtcggatt gctttcggtt ctcttacagt gaagacgaca aggaatggag gcatctgaag
     2581 accatcccca gaccggactc cttggcgggt gctccttccc tgatggtctt tggcaactac
     2641 tacggagaag acgacgtcct gccctttggt gatgttgcca tgtccccaga cggagatcgg
     2701 ggttcgaaat acacctgttt cgtcggcgaa atccgaatcg aaaagacgcc tgaagatgcg
     2761 cttctgatga cttcagcaga gaaaagggcg attgagacag aaggactcga ccccgttgcg
     2821 caggttgtgc ttcgaggttc cgaagatccg acctacgaat ctgttgaggc tttctatcca
     2881 cccttcaagt ttccccgcga agtggtcgga gtgccggagc atccgcttga tatcggaata
     2941 gattatcaag ggcggctgga tgtcagtccc tgggatcgtc catacgcatg gatcgaaatt
     3001 ggcgatccgc tgaagccctt tggagatacg ccaatagcga ggcgactgca gaaaggttac
     3061 cttcccattg tgacgctgtc cacggagcga gatgacattc ggtatgagat ggccgttttc
     3121 ggctggtctg aggacttccg agtggacaag gatctctttg cctacgtgcg actgcgcgca
     3181 agaacatcga aaagtggcca ggaggtgaag ccgattgcgc ttgtggacgc acggagcggc
     3241 gcacgaaccg agtggccgtt gttgaagatt ggcagaagag attacgaagt ctgtctgtcc
     3301 ttcaaatatc ccgaaccctc ttcagcccaa cgggtttcct cttcagcttt cgaacgcatc
     3361 tccaggaacg tgacagcgca ttgggagaaa cgtctcgaaa tagccaagag agtccgtgtt
     3421 ccagaccagc gaatgaacga agcatacaag gcatggatcg tctactcact gctcaatgct
     3481 gacactgtca acggctacgt cgaagcgcgt gacggttcgg gtttctatga cgaaatgttt
     3541 ggctacagtg tttcgctgca cacgatggcg gcggatctgt tcggaatgca caactacgcg
     3601 gagcgatgtc tccagaccca gctgcatttc caagcagaag acggcatgta cgtgcaggca
     3661 acagggcttc cggatctcgg atctttgctg atggcgctct atgaacacta tctggtcaca
     3721 cgagatgttg cttggctgca atcggttgca ccaaacattg ataagggctg ccaatggatt
     3781 ttgcagcaac gccaagcgac gcccaaagag ggaatcacaa aagggatgat caaattccgc
     3841 ccctactgtg actatccggt acccgtctac aacttcatgg cggacatcta tgcctgtatg
     3901 ggtttggagc gaggagcaga ggctctgcag gttctcggaa tgcggtcgga agccgagcga
     3961 tacgccgccg aggcagatgc gtatcggaac gacctgctgg ctgcgatgga cgcagccgct
     4021 ttccaggatg gcgatctgaa gatacttccc atggaacccg aaacgcggag actgctgaaa
     4081 ctcgatcagt atcagggcgg cggatactat ggtttggtat gcggcatcct tctggagacg
     4141 ggatttctta ctccggacga tgttcgctcg cactggatca ctgatatcat ggagagaagg
     4201 aacggcctga tcggtggcgt ctgtgaattt caactgggtg ttgatcatgc ctacacctat
     4261 ggctatctca tgacaaagat gcagcgggac aacattcgaa acgtgattct cgggctttgg
     4321 gggatgatgg cttttgggat gactcgtgac acctattctc ctgtcgaggt caatatgatt
     4381 caaacgggag acaatcacct gacactgcca cacctttact cctgcacaca acagttgagg
     4441 ttgctgcgca acatgctttt gtgtgaagag ggtgatgttc tgtggatcgg cagggcgatt
     4501 ccccgagtct ggttggaacc ggacaaaaga gtagaagtca agtcggcgcc gacccatttc
     4561 ggtgaagtaa gttatagcat tgatgcgaaa cgcaatggga atatggtcgt gcgtatcagc
     4621 ccac
//
LOCUS       DAOQUC010000116         4601 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_20874_length_4601_cov_3.593049, whole genome shotgun sequence.
ACCESSION   DAOQUC010000116 DAOQUC010000000
VERSION     DAOQUC010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4601
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20874_length_4601_cov_3.593049"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            113..1211
                     /gene="prfB"
                     /locus_tag="PKH07_03120"
     CDS             join(113..169,171..1211)
                     /gene="prfB"
                     /locus_tag="PKH07_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_095522723.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HNT33969.1"
                     /translation="MFEEQLRQAREAAEGIERLEAFFDPDGLRKKIEDYEGQVSIPGF
                     WNDSEKAQRITRELKQAQDLLAQYTSLQSRAADVIELIEMSAEEEDAESQGVLDSEIA
                     ALEKDVSAFFLRRALTGEHDGGNAFLSIHPGAGGTESCDWAEMLLRMYLRWCERRGYP
                     TEVVDIQPGDEAGIRNVTILVRGENAYGYLHSESGIHRLVRISPFDANKRRHTSFASV
                     YVMPELDDNVDVDIRDEDLRVDTYRSSGAGGQHVNTTDSAVRITHLPTGIVVSCQNER
                     SQHKNRATAMKVLRARLYEVRLQEQQDKLDALAAGKKDIRWGNQLRSYVFCPYQMVKD
                     HYTGQETGNVGAVMDGEIDDFIDAALREKIS"
     gene            1233..3686
                     /locus_tag="PKH07_03125"
     CDS             1233..3686
                     /locus_tag="PKH07_03125"
                     /inference="COORDINATES: protein motif:HMM:NF021379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2079 domain-containing protein"
                     /protein_id="HNT33970.1"
                     /translation="MSFQSEAPRRIPLVLASFFAAGVLLGIVGWRCARLNPTESVAVC
                     HEFKPGDQSLPRDFIEQVGSLPVAWLLTGQGYPLEGIPEGVFEVKLSVVMPSDWDPMQ
                     CPLVLVTPDGEKLLDLEEPPRPLTETHQKIRYSGRVTWSQFGPFGIRCAAEADTVGVL
                     SLRLATYAGYASKIGYFIFQFPSGTMHLYAPATVGLIGLLFWGWLFSWAKFLFFSERW
                     DSLRQRLFILFVWLGPGTLALVELVSTAFTPCRLILTPFGLVSVGIAPMFVGWLVITL
                     GWGEKPVVPDPFNQAGGFLLRCLRGISVKAHLDWGVAVVALAGLVFAVLVSCMSFYRH
                     AEFHTHAFDLGVVSNDIWNTAHGRLFETSLLPWSHLGEHFALVYLFLAPLYWIWADPR
                     MLIAVQAVFLGLSAVPLYVFGRKILGRKWLAALVAVGYLNHPGLRGIALSEVHQIGFY
                     PMFFLAAMCLLFDGAPETWQRRRPRHLLAFLIVIALAVSIREDLALAAMFLGLYLMTQ
                     RGRRLFGLQLSVGSLVCFLLLVLVVIPYFRGQPYSHIQRYDEMGGTFSGIASNLFLHP
                     LQSFRLMMSEQRSTLIREALKPVLYLPVLSGWAFILVLSSLGGCLLSSNAYVFSLFTH
                     YPGLWIAPIYVTTILALHQVFRRMERFRSSGSSLRIVLRIVGLVFFLVLIVAALFPSS
                     SVPPWKTVASRSRWEKVYRRQSFSEPARTLLKRFDQPEASVSAQAMFVPHLANRREIY
                     HFPDGGRTDYLLVDIGLESTKYPFMDVDFRKILIERIEEGNYGLVDREATVCLLKRDA
                     PRTDADAALIQDIKTWAIE"
     gene            complement(3760..4125)
                     /locus_tag="PKH07_03130"
     CDS             complement(3760..4125)
                     /locus_tag="PKH07_03130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33971.1"
                     /translation="MNRKRFVVVALAFAVVFGAVKSLSQESVRPQWEYGIYSERTEGN
                     EQRYEWQSKGLTLLTKKRTELLEKMGYKPAEGEEAMFPLVKMLDFFGANGWEMVQVSP
                     DSPYATMRYYFRRPVLSQS"
BASE COUNT          951 a         1209 c         1235 g         1206 t
ORIGIN      
        1 ctgtttgcgc aacaggcatt ggttatgcca tacttccttc aagtctgttc agggtgactc
       61 gcctcgtcaa agaggctgga agcctcttct acatgagagg agtcgtttgg gaatgtttga
      121 agaacagtta cgtcaggcac gtgaagccgc tgagggtatt gagcggctgg gaggctttct
      181 ttgaccctga cggtcttcgg aagaaaatcg aggactacga gggacaggtc agcataccgg
      241 ggttctggaa cgattcggag aaggcgcagc gcatcacccg tgagttgaag caagctcagg
      301 atctgttggc gcagtataca tcgctccaga gtcgcgccgc agatgtcatc gaactgattg
      361 agatgtctgc tgaagaagaa gacgccgaat cacaaggtgt gctcgacagt gagatcgcag
      421 cattggagaa ggatgtcagt gccttctttc tccgcagagc actcactgga gaacatgatg
      481 gcggcaacgc ctttctctcg atacaccccg gtgcgggtgg caccgagtcc tgtgattggg
      541 cggaaatgct tctgaggatg tatctccgat ggtgtgagcg ccgaggctat ccaacagaag
      601 tcgttgacat ccagcccggt gacgaagcgg gaattcgaaa tgtcaccatt ctggttagag
      661 gagagaacgc ctacggatac cttcattccg agagtggcat tcatcgcctg gttcgcattt
      721 cgccattcga tgccaacaag cgccgtcata cctcctttgc ttcggtctac gtgatgcccg
      781 aactcgatga caatgtggat gtggatattc gggatgaaga tttgagggtg gacacgtacc
      841 gttcctctgg agccggtggt caacatgtga ataccacgga ctctgccgtt cggatcaccc
      901 accttccgac gggcatcgtc gtctcctgtc agaatgaacg ctctcagcac aagaaccgcg
      961 cgacggcaat gaaggtgctt cgcgctcgtc tgtatgaagt gagacttcag gagcagcaag
     1021 acaaattgga cgcgcttgcc gctggaaaga aagatatccg ctggggcaat cagctgcgct
     1081 cctacgtgtt ctgtccgtac cagatggtca aagatcacta tacgggtcag gagacgggca
     1141 atgttggggc agtgatggac ggcgagattg acgacttcat tgacgcggca ctccgcgaga
     1201 agatttcctg acaggaagac cggtaccgcc acatgtcttt tcagtctgaa gcaccgcgcc
     1261 gaattcctct tgttctggca tcattctttg ctgctggagt tctgctgggg attgtgggtt
     1321 ggcgctgtgc tcgtttgaat cccaccgaat ccgttgccgt ctgtcatgag ttcaaaccag
     1381 gagatcagag cctgcccagg gatttcatcg aacaggtggg cagtctgccg gttgcctggt
     1441 tgctcacggg acagggatat cctcttgaag gaatccctga aggagtcttt gaggtcaagt
     1501 tgagcgtcgt catgccctcg gattgggatc cgatgcaatg tcctctggtt ctggtgactc
     1561 ccgacggtga gaaacttctg gacttggaag aaccgcctcg acctctcacg gaaacgcacc
     1621 agaagatccg ttactcggga cgagtcacat ggagccagtt cggccctttt ggaattcggt
     1681 gtgcagcgga ggcggacacg gtaggcgtgc tctcattgag actggccacg tacgcaggtt
     1741 acgccagcaa gattggctac ttcatctttc agtttccatc agggacgatg catctctatg
     1801 ctcctgccac ggtgggtctc atcggcttgc tcttctgggg atggcttttc tcttgggcaa
     1861 agtttctgtt cttctctgaa cgatgggatt cactgcgcca gaggcttttc atcctgttcg
     1921 tatggctggg acctggaacg ttggctttgg tagagctagt cagcactgca tttactccct
     1981 gccgacttat cctgacacct ttcggtttgg tgtcagtcgg cattgctcca atgtttgtcg
     2041 gttggttagt cataacactt gggtggggtg agaaacctgt tgtcccagat cctttcaatc
     2101 aagccggtgg gtttctcctg cgctgtttga ggggaatctc cgtcaaagct catcttgact
     2161 ggggcgtcgc agttgtcgcg ctggctgggc ttgtcttcgc ggtattggtt tcctgcatga
     2221 gtttctaccg tcacgcagag ttccatacac acgcctttga tctgggcgtt gtcagcaacg
     2281 acatttggaa cacagcgcac ggaaggcttt ttgagacgtc attgctgccg tggtctcatc
     2341 tcggcgagca tttcgcactg gtgtatctgt ttcttgctcc tctctactgg atctgggcgg
     2401 atccgagaat gctgatcgcc gtgcaggccg tgtttctggg tcttagtgcc gtacctctgt
     2461 atgtattcgg tcgaaagata cttggcagaa aatggcttgc agcactcgtt gccgttggat
     2521 acttgaatca tcctggactg cggggcattg cgctgagcga agtgcaccaa atcggtttct
     2581 atccgatgtt ctttttagcc gcaatgtgtc ttctttttga tggtgctccc gaaacgtggc
     2641 aaagacgacg gccacgccac ctgcttgctt ttctcattgt gatcgcactt gctgtcagca
     2701 tccgtgagga tcttgctctg gcggcgatgt tccttggctt gtatttgatg acgcaacgcg
     2761 gacgccgact tttcggcttg cagttaagtg tggggtcact cgtctgcttt ctcctcttgg
     2821 tactggttgt gattccgtac ttccgcgggc agccgtacag ccacatccaa cgctacgacg
     2881 aaatgggtgg taccttcagc ggtattgcca gcaatctgtt cctgcatcca cttcaaagtt
     2941 tccgcctcat gatgagtgaa cagcgctcga ccttgattcg ggaagccttg aaaccagtgc
     3001 tctatcttcc cgtcctttcc ggctgggcat tcatcctggt gctgagttct ctggggggat
     3061 gcctgctctc ttccaacgct tatgtcttct cgctcttcac gcattacccg gggttgtgga
     3121 ttgcaccgat ctatgtgacg actatcctcg ccctacatca ggttttccga aggatggaaa
     3181 ggtttcgttc atccggcagt tcgttgcgca tagtccttcg tatcgttggt ctggttttct
     3241 tcctagttct catcgtggct gcgctttttc cctcgtcttc ggttccacct tggaagactg
     3301 tcgcgagtcg ttcccgatgg gaaaaggttt acagacgtca gtccttcagt gagccagcga
     3361 ggactctcct caagcgattc gatcaacccg aggcatccgt ttccgcccag gcaatgtttg
     3421 ttccgcacct ggcaaaccgt cgggagatct accacttccc tgatggagga agaaccgatt
     3481 acctgctcgt ggacatcggt ctcgaaagca ccaaataccc gttcatggat gtggatttcc
     3541 gtaagattct gatagagcgt atcgaagaag gcaactatgg cctggttgat cgagaagcga
     3601 ctgtgtgtct tctaaaaagg gacgctccga gaaccgatgc tgatgctgcg ctgattcagg
     3661 acatcaagac ctgggccatc gagtgagcgt caaatccgaa tcgcctgtcc tggcgatact
     3721 ccccacaaat agtctacggc aaacacactg agagcaggat cagctttgag atagtaccgg
     3781 ccgtcggaaa tagtaacgca tcgtcgcata gggcgagtca ggagagacct gaaccatctc
     3841 ccagccattg gcgccaaaga agtccaacat cttcaccaac ggaaacatgg cttcttcccc
     3901 ttcggctggt ttgtaaccca tcttttcaag aagttctgtc cgcttcttag tcagcagggt
     3961 taaccccttg gactgccatt cgtagcgctg ctcattgcct tctgtgcgtt cggagtaaat
     4021 gccatattcc cattgcggac gcacactttc ctgactcaaa gacttcacag ccccaaagac
     4081 aacagcaaaa gccaaggcca caacaacaaa acgcttccga ttcattccga actcctttca
     4141 cgcttgggga agcgaagatc acttcccaaa aagaccgtat gattcacgcc ttttagtata
     4201 ctccgggtct ggcatcagtg ccagaaaatg aaagtttcgc agcaccgttc ccttcttcca
     4261 ctaccttgac aagtgacccc ctttcaattc tacttatttt gttcgtatca tgtggactgg
     4321 atgaggtagg ttgaattgcg acagaacatc cgcccgctct gtatcctgct tgtctgtctt
     4381 gctctgctga gtttgtcctg cgctcgaaag gagtcatcgg aacagatgag agccgaatgc
     4441 attccatttc catcgtcaga tggcacaaat actttctacg ttggtaaccg cgatccattg
     4501 atctccagcc cactcatgcc attgcccgtg ggttgtgttc gtgcgcacgg ttggctcctg
     4561 catcagctgg agatcatgcg tgacggcatg gtcggccatc t
//
LOCUS       DAOQUC010000117         4586 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_20956_length_4586_cov_2.859854, whole genome shotgun sequence.
ACCESSION   DAOQUC010000117 DAOQUC010000000
VERSION     DAOQUC010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4586
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20956_length_4586_cov_2.859854"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..176
                     /locus_tag="PKH07_03135"
     CDS             <1..176
                     /locus_tag="PKH07_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005571634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HNT33972.1"
                     /translation="GDAFNGALAVALSRGESLLSAVRFAHAVAALSVTRLGAQPSLPH
                     TSEVTTFLENHGS"
     gene            complement(187..846)
                     /locus_tag="PKH07_03140"
     CDS             complement(187..846)
                     /locus_tag="PKH07_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020267614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNT33973.1"
                     /translation="MLLRASDISKRFQKKETSVQALDHVSLELANGEFVAIHGPSGSG
                     KTTLLLLAGGLLAPDKGEIFLNGKNPYKMMPDQRARFRAETVGFVFQQFHLIPYLTVL
                     ENVMAPSVALPMPERVERGKELLDRFAMLDRADHVSAELSTGERQRTALARALFNKPA
                     LILADEPTGNLDDENASVVLKTLSEHAAQGGGVLLVTHDPKAASLAHRTLHLRQGALV
                     Q"
     gene            complement(899..1537)
                     /locus_tag="PKH07_03145"
     CDS             complement(899..1537)
                     /locus_tag="PKH07_03145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33974.1"
                     /translation="MRKEPVIVSLLIVVSLFFAEPVCARDFRVSRIPNAGVFLCGTCH
                     LNPVSPGARNPFGQTVEELLPSLDEDFWGADLAAGDSDADGFTNGFELQDPFGTWRPG
                     NPNPGNSSLVTNPGDASSFPLVSPTPTPSSTPLPDFTDTPTPVPTDTESPTQTPPEPT
                     ATETPTETPVEPTATDTPVEPTVTPRSPDLNGDGVVDQQDLYILMEQWHSEL"
     gene            1855..3684
                     /locus_tag="PKH07_03150"
     CDS             1855..3684
                     /locus_tag="PKH07_03150"
                     /inference="COORDINATES: protein motif:HMM:NF013400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33975.1"
                     /translation="MNESVLWRLKCADEAGASSPSYDDSGWEQIQLGDKFASTGFAWL
                     RARFVIPKSVLGERVKGKPIALRLNCGDGEVYIDGQFYGRYDNDHPAMILLSETAEPG
                     KEYMVAIRLYMSNHANLEGTYTLEECSWVIHDPRRVRDAFRLTVDCSRTEGRLWQPFS
                     GLSQGAGMQDYDPGTAAVFREIGIKWFRMDNILTQAVKRGEDGKLIYDWTDVDRRTDF
                     IKSIGAEPIYCLSYMPKVLDATDCPHRHSRPNDWDAWEELCYQAVKHCVDRGTPVKYW
                     EVWNETNTGWITVLPGEDLLTVYLQMYDRCARAVKRADPNALIGGPCNAAGPWDDGDK
                     IGLPGVRGEAFLRGLIQHCSQTNTPLDFISWHEYFHPWWIMVREVERTREILAEYPET
                     QKNVKELMVTEWSFAWWHDLPHDNEIGAAYCAAGAIRAWSAMKVDKPCYFFAKDGSPP
                     FHGNWGMLTANNTPKAVGNVCRMFNMMAPNALEIQGFEKDPSGNSDPQDTDLCGLASI
                     DSETGRMTILLVNFPNRHGIPRKIALELRHLPESLRSGTLRRWLVDKQHSNVFHDLTK
                     GRLEVVDESPLGNRSSIHYQFALDQNGVTLIEFLPANTKELEN"
     gene            3686..>4586
                     /locus_tag="PKH07_03155"
     CDS             3686..>4586
                     /locus_tag="PKH07_03155"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNT33976.1"
                     /translation="MTSKQRMLAVLDGKSVDAIPVAPHWWGVYKHEIAETDGITAAST
                     ANGEHRVSHLTEVDAHFYQVFSPDWFHLGAAEWHLDPYAERQEDRQNLIERLQRLNDA
                     TVIEDYLAVEALTEEDIRKTGTYEHVREIVKRYGGDVFVAVNEGNPVCGVFDPHGLIG
                     FERGLVALVEKPHLMERLLFGLYDLRLTWMRVLADFGCHAYIGSETYVGADLISPNAY
                     RSVIFPAQEHFYKSLSVIGLIPIAYYCGDVVPMIPVINELPIKALMVEESRKGFELDP
                     VNIRQKLAESITLFGNLDTVSTLR"
BASE COUNT         1100 a         1220 c         1239 g         1027 t
ORIGIN      
        1 caggcgatgc cttcaacgga gccttggcag tcgctctatc gcgaggggaa tctcttctgt
       61 cagccgtccg gtttgcacat gcagtcgccg cgttgtcggt aacacgcctc ggagcgcagc
      121 cgtctttacc ccacacatcc gaggtaacca catttctgga gaatcacgga agttgatttc
      181 gactgcttac tgaaccagag caccctgtct tagatgaagc gttctgtgag ctagagacgc
      241 agccttggga tcatgagtga ccaggagtac acctccgcct tgggctgcgt gttccgaaag
      301 cgtcttgaga acaacggaag cattctcgtc atccagattt cccgtcggtt catcggcaag
      361 aattagcgcg ggcttattga acaacgctcg cgccagtgct gttctttgcc tctctcccgt
      421 gctcagctcg gcggaaacat gatcagcgcg atccaacatg gcaaaccgat caagcaattc
      481 cttgcctcgt tccacacgtt ccggcatggg cagagcaacc gaaggtgcca tcacgttctc
      541 aagaactgtc agatacggga tcagatgaaa ctgctggaag acaaaaccaa cggtttcagc
      601 ccgaaatctc gctctttggt caggcatcat cttgtaagga ttcttaccat tcagaaatat
      661 ctcacccttg tcaggagcaa gcaggcctcc cgccagcaga agaagcgtgg tcttcccgga
      721 accactcgga ccatggattg cgacaaactc accgttggca agttcaagag aaacatggtc
      781 aagcgcctga acgctggttt ccttcttctg aaatcgtttt gaaatgtcac tggctcgcag
      841 cagcatgccg gctcctcaag agattttgta ccccacgacc gattcagacc ctgacgggtc
      901 agagttcact atgccactgc tccatcaaga tgtagagatc ctgctgatca acaaccccgt
      961 ctccgttgag atccggtgat cgaggagtta cggtcggctc aaccggagta tctgttgctg
     1021 tgggttccac cggagtctct gtcggtgtct ccgtggcagt cggttcgggc ggcgtttgag
     1081 tggggctttc tgtatcggtc ggcactggtg ttggtgtatc agtgaaatct ggaagcggcg
     1141 tactgctggg tgttggcgtc ggtgacacga gaggaaaact gctcgcatcg cccggattgg
     1201 tgacaagact ggagttgccc ggattgggat tccctggacg ccaagtccca aagggatcct
     1261 gaagttcaaa accgttggta aaaccgtcag catcagagtc tccggccgca agatcggctc
     1321 cccagaagtc ctcatcgaga gaaggaagca gttcctcaac agtctgtcca aaaggattgc
     1381 gtgctccagg agagacggga ttcagatggc acgtcccaca aagaaaaaca cccgcgttag
     1441 gtatcctaga cactcggaag tcccttgcac aaacaggctc ggcaaaaaag agagacacca
     1501 cgatcagcaa tgaaacaatg acgggttctt ttctcatgac caaccacctt tccatgcggc
     1561 tgcagcgtac tctctgtagc acgcctggct cgatttgctg cagcacgtca caaaccttgc
     1621 ccatgtatcg aagtcactgt atcgcattgt ataacatcgt gtcaatcctc ggccttcggt
     1681 gaactgaatc atgtgcccgg tacccaccgc atttgacaaa acagtctcaa gaaagcagga
     1741 ttcaagacaa acgacaggaa aggggcttac gggttgaaga gtttgttggt attgttggct
     1801 tgcatatcgt tccttgttct cttgccctgc ctgtgcgcgg cagaagaggt ttttatgaac
     1861 gagtctgttc tatggcgtct caaatgtgcg gatgaggcgg gagcgtcttc tccatcctat
     1921 gatgattcgg gctgggagca gatccaactc ggagacaagt ttgcctcgac gggatttgcc
     1981 tggcttcggg cgcgattcgt gattcccaag agtgtcctgg gagaacgcgt caaaggcaaa
     2041 ccgatcgctc ttcgcctgaa ctgcggcgat ggagaggtat atattgacgg tcagttttac
     2101 ggtcgctacg acaacgatca tccggcaatg attctgctgt cagaaacagc agagcctggc
     2161 aaggagtaca tggttgcgat ccgtctgtat atgtccaacc atgcgaatct cgaaggaacc
     2221 tatacgctgg aggaatgctc gtgggtgatt cacgaccccc gtcgcgttcg tgatgcattt
     2281 cggttgactg ttgactgttc gagaaccgaa gggcggctct ggcaaccttt cagcggcctg
     2341 tcccaaggtg ccgggatgca ggactatgat ccggggaccg ctgctgtctt tcgagagatc
     2401 gggatcaagt ggttccgaat ggacaacatc ctcacgcaag cagtcaagcg aggtgaggat
     2461 ggaaaactca tctacgactg gacggatgtg gatcggcgaa cggacttcat aaaatccatt
     2521 ggtgcggaac cgatctactg tctgtcctac atgccgaaag tcctggatgc cacagactgc
     2581 ccacatcgcc acagtagacc caacgactgg gacgcctggg aagaactctg ttaccaggca
     2641 gtcaagcact gcgttgaccg cggaactccg gttaagtact gggaagtctg gaacgagacc
     2701 aacacagggt ggattaccgt cctgccggga gaggatcttt tgacagtcta tctccagatg
     2761 tacgaccgct gtgcccgagc cgtcaaacgc gccgatccga acgcgttgat cggcggaccc
     2821 tgtaatgcgg caggcccctg ggacgacgga gacaagatcg gactcccggg agttcgtgga
     2881 gaggcgtttc tgcgcggact cattcagcac tgctcacaga ccaacactcc tctcgatttc
     2941 atctcatggc atgagtattt ccatccgtgg tggatcatgg ttcgggaagt ggagcgaacg
     3001 cgagagatcc ttgccgagta ccccgaaact caaaagaacg tcaaggaact catggtcacg
     3061 gaatggagtt tcgcctggtg gcatgatctg cctcacgaca atgagatcgg agccgcctac
     3121 tgcgccgcag gagcgatccg agcctggagt gccatgaagg tggacaagcc ctgctacttc
     3181 ttcgcaaagg atggcagtcc accgtttcac ggcaactggg gaatgctaac cgcaaacaac
     3241 actcccaaag ccgttggaaa cgtgtgccgc atgttcaata tgatggcgcc aaatgcactg
     3301 gagattcagg gcttcgaaaa agatccaagt ggcaacagcg atccacagga taccgatctc
     3361 tgcgggctgg cttcgatcga ttcagaaact ggccgaatga cgattctgtt ggtgaatttc
     3421 cccaaccgac acggaatccc tcggaagatc gctcttgagc tgaggcacct tcccgaaagc
     3481 ctccgcagtg gcacattgcg tcgctggctc gtggacaagc aacacagcaa cgtatttcat
     3541 gatctgacaa aaggaagact cgaagtcgta gacgaatctc cactggggaa tcgttcttcc
     3601 atacactacc agtttgcact cgatcagaac ggcgtcacgc tcatcgaatt cctgccagcc
     3661 aacacgaagg aattggagaa ctgacatgac gtccaagcaa cgaatgcttg cagtactgga
     3721 cggcaaatca gtggatgcca tccccgtcgc gcctcactgg tggggtgtct acaagcacga
     3781 aattgccgaa accgacggta taacggccgc gagcactgcc aatggggagc atcgagtctc
     3841 acatcttacg gaggttgatg ctcatttcta ccaagtgttc tcaccggatt ggtttcatct
     3901 tggcgctgcg gaatggcatc ttgatccata cgctgaacgg caggaggaca gacagaatct
     3961 gattgaacgt ttgcagagac tcaatgatgc gactgtcatc gaagactatc tcgccgtcga
     4021 agcactcaca gaagaggata tccgaaagac cggaacctac gaacacgttc gagagattgt
     4081 caagagatac ggcggtgacg tcttcgttgc ggtcaatgaa ggcaatccgg tctgcggagt
     4141 tttcgatcct cacggtttga tcggcttcga gcgaggactt gtcgctcttg tggagaagcc
     4201 acatctgatg gagagactgc tctttgggtt gtatgattta cgtctcacct ggatgagagt
     4261 cctggcggac tttggctgcc acgcatacat cggatcggag acatacgttg gcgccgacct
     4321 catatcaccc aacgcctatc gttctgtaat cttcccagcc caggagcact tctacaagtc
     4381 tttgagtgtg ataggactca tccccatcgc ctattattgc ggtgacgtcg tgccgatgat
     4441 ccctgtcatc aatgaactac cgatcaaagc attgatggtt gaggaaagca gaaaagggtt
     4501 tgaacttgat ccggtcaaca tccgccagaa attggccgaa tccataacgc tgttcggcaa
     4561 tctcgatacg gtgtcaacgc ttcgtt
//
LOCUS       DAOQUC010000118         4584 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_20965_length_4584_cov_3.763524, whole genome shotgun sequence.
ACCESSION   DAOQUC010000118 DAOQUC010000000
VERSION     DAOQUC010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4584
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20965_length_4584_cov_3.763524"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..317
                     /locus_tag="PKH07_03160"
     CDS             <1..317
                     /locus_tag="PKH07_03160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33977.1"
                     /translation="SCGFLTWMDIMERHHCMCWKVKGLRELVDFPDIYSLIAPRFLQC
                     QNGLKEPINQFHPIMARRAFECVKQAYDDVGTPGNAQLHIHGEGHVVDVSALVGFITA
                     HL"
     gene            338..1519
                     /locus_tag="PKH07_03165"
     CDS             338..1519
                     /locus_tag="PKH07_03165"
                     /inference="COORDINATES: protein motif:HMM:NF018743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HNT33978.1"
                     /translation="MQRKTTVLTGLDHELSEGCPNLTDQRIGMVCNHTAVTAQGEHAV
                     DALLEAGVHIVRLFGPEHGFRGEVPDGIKIESDRDALTGLPCTSLYGQRLRPTAEELK
                     EIDLLVYDIQDLGVRFYTYIWTLRECMAAAAESGTPFLVLDRPGIVGSAIEGNVLDER
                     FSSFVGQKPLAWRYAMTPGELATLYSRQGWLGYSQNMDLKVVKMLNYQRGIWADETGL
                     PWIAPSPNIPTPTSCIHYPGTCLFEGFSTVTEGRGTEFPFEIVGAPFVNGEDWAKAME
                     QFDLEGIEFEPAVFTPRSIPKKVDKPKFMDDKCGGLRLVCSDRSTYSPVRVGIALIKT
                     LLELYPDQVVFRELHFDRLSGTDQLRLNLLAGKTLDEIMLSLESSLPGFLPIREACLL
                     Y"
     gene            1812..2042
                     /locus_tag="PKH07_03170"
     CDS             1812..2042
                     /locus_tag="PKH07_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014108323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HNT33979.1"
                     /translation="MSTERWIRLIAGTLVLLSLALGTWVNHNWYWFTAFVGANLFQSA
                     LTRWCLMEDILRKVFGVKSESELQACCSKESK"
     gene            2093..3145
                     /locus_tag="PKH07_03175"
     CDS             2093..3145
                     /locus_tag="PKH07_03175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33980.1"
                     /translation="MNYPVVVRVDANAPIGMGHITRCHVLANALQERGLAPALFLTRD
                     YSAAVEFLESRRLPYRILGAEMDFASECAEMRRTALDVGARVLLFDLLDVTEELVEKT
                     CPEGVLSVVLLDHLDPPTIRKGLVFNHNVVYGASTHPTRSQQDYLAGASYVLLDPVFA
                     EASRKSYFCESVSRLFLNQGGGDPFNLTTKILRALTPLRDQLKIEVVLGGAFAFDEEL
                     APVLKHWGQQVTLHRMVPPAQVAALMMQAQMAHTAPGNMLYELLTLGVPSIAISHHEK
                     HAAVAEFFAVQGAIEHLGIGVTLSEVLILERTRSLLHDEERRKSLHLVGRKIVDGLGA
                     VRTADAILKALHGEPT"
     gene            3280..>4584
                     /locus_tag="PKH07_03180"
     CDS             3280..>4584
                     /locus_tag="PKH07_03180"
                     /inference="COORDINATES: protein motif:HMM:NF028468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE3 domain-containing protein"
                     /protein_id="HNT33981.1"
                     /translation="MSRQDDLVRRYGSMALFVLTLGIIIATRLHSFLLFHSLAEIFII
                     AATLVICLVALNTRNFQDNPYFLFLGTGLFFVGLTHSLHLLAYEGMSVFTTGGSNLAA
                     QLWIIARGIEGVTIFLAPFSLNFRFRLRYIFFGFLLLTTLLLASVFYWKVFPLCYVDG
                     VGLTVFKIVAEGVVCVLTILASLALLLKRNLFCPRVLFLLLVSFALTLLAGVLFTLYI
                     NPYSGLNLLGHVLTIASFYLLYRAVVEFGLLTPYETMFRNLKHSETALRASEEELQTI
                     FESASIMMVLADEHHCLRRINRSAETFSKKARSDVLDARPGEAFGCLGALENPQGCGY
                     GALCETCILRNTVLDTFAKGTAHVRVQADVPFHGAGRSGTVSLLVSTSLLHISDRPMV
                     LVCMEDISQLREVQREREKMIHDLQEAAAQIKTLSGLIPICSS"
BASE COUNT          973 a         1152 c         1263 g         1196 t
ORIGIN      
        1 ccagctgtgg attcctgaca tggatggata tcatggagcg ccaccactgc atgtgctgga
       61 aggtgaaggg acttcgggaa ttggttgatt tcccggacat ctactccctg atcgcgccac
      121 gttttctgca atgtcagaat ggactgaaag aaccgatcaa ccagtttcac ccgatcatgg
      181 cgcgtcgtgc gttcgagtgc gtgaagcagg cctatgacga tgttggcacg cctgggaacg
      241 cgcaacttca tattcatgga gaaggtcatg ttgtggatgt ttcggcgctg gttggattca
      301 tcactgcaca tctataggtt tttgggagca gaatatcatg caaagaaaaa caactgtctt
      361 gacggggctg gatcacgagt tgagcgaagg gtgtccgaat ctgacagacc agagaattgg
      421 tatggtctgc aaccatacgg cggtcaccgc tcagggggaa cacgccgtgg atgcgcttct
      481 cgaagcgggt gtacatattg tccggctctt tggtcccgag catgggtttc gaggtgaagt
      541 cccggacggc atcaagattg aatcagacag agacgctctg acgggtctgc cctgtactag
      601 cctctacggt cagcgccttc gtcctactgc cgaagaactc aaggagatag accttctggt
      661 ctacgacatt caggatcttg gcgttcgttt ttacacctac atctggacgc ttcgtgagtg
      721 tatggcggcg gcggcggaat cggggactcc cttcctggtg ctcgaccgac ctggaattgt
      781 cggctctgcg attgagggga atgtgctcga cgagcgtttc tccagtttcg tcgggcagaa
      841 gcctttggca tggcgctacg cgatgacgcc tggcgaactg gcaacgctgt acagccgaca
      901 agggtggctt ggctattcac agaacatgga tttgaaagtc gtcaagatgc tgaactatca
      961 gcggggaatc tgggcggacg aaacgggact tccctggatc gcaccctctc cgaacattcc
     1021 aacgcccacg tcctgcattc actacccggg aacctgcctt ttcgagggat tctccacggt
     1081 cacggaagga cgaggcacgg agttcccgtt tgagattgtt ggtgcgccgt ttgtcaacgg
     1141 agaggattgg gcaaaagcga tggagcagtt cgatctggaa ggcatcgaat tcgaaccagc
     1201 ggttttcacg ccgagaagca ttcccaagaa ggtggacaag cccaagttca tggatgacaa
     1261 gtgtggtggt ttgcgtctgg tctgttccga ccgcagcaca tattcaccgg tgcgcgtcgg
     1321 cattgctctg atcaagaccc ttctcgaact gtatcccgat caggtcgtct tccgtgaact
     1381 tcatttcgac cggttgtctg gaacagatca actgcggctt aatctgcttg ccggcaagac
     1441 ccttgatgag ataatgcttt ctctggaatc gtcgttgcca ggatttctgc cgatacgaga
     1501 ggcctgtctt ctttactgaa cggagaggcc tcagcccgtg cactcgcgag gcgacaaact
     1561 ggaaagggca ttggacatag agtgtccgtc ggggcatatt ccgtagaatc ggaaggatgc
     1621 ggctctctat caacagatag ctgccatgca ccagtatctc ggccgtaatg gcaaagtggc
     1681 acaatggcag tacagagcgt cggaagcgta tgaatgtgac tggaagaccg ccgtcggctt
     1741 tgacatcgtc tttccgctgt ttctatactt tgcccggtac aggccggtct ttcagaaaaa
     1801 ggagttatgc catgtcaaca gaacgatgga ttcgtctgat cgctggaacg ctggtgctcc
     1861 tgagtctggc actcgggact tgggtgaatc acaattggta ctggttcacc gctttcgttg
     1921 gtgccaattt gtttcagtcc gcactgactc gatggtgttt gatggaagac atcctgagga
     1981 aagtgtttgg tgttaagtcc gagtctgagc tccaggcctg ctgctcgaag gaatccaagt
     2041 agaagagttt cacaacgagg ctcatcgtga cagatgaatc cttggagaag gaatgaacta
     2101 tcctgttgtt gttcgtgttg acgccaacgc cccgattggg atggggcata tcacacgctg
     2161 tcatgtgctg gccaatgcac tgcaagagcg tggactggct cctgccctgt ttcttaccag
     2221 agattattcg gcggccgttg agtttctcga gagccgtcgt ctcccttatc gaattctcgg
     2281 cgcagaaatg gattttgcgt ccgagtgcgc ggagatgcgc cgaactgctc tggacgttgg
     2341 ggcacgggtg ctccttttcg acctgctgga tgtgaccgag gaactcgtgg aaaagacctg
     2401 tcctgagggg gtgctttcgg ttgtgcttct ggatcatctt gatcctccaa caatacgcaa
     2461 gggtctcgtt ttcaaccaca atgtggtcta cggtgcaagt acccatccaa cccgctctca
     2521 gcaggactat ctggctggcg catcgtacgt tcttctggat cctgtttttg cagaggcatc
     2581 caggaagtcc tatttctgcg aaagcgtctc acggctcttt ctcaatcagg gaggcggcga
     2641 tccattcaat ctgacaacaa agatacttcg cgccctcacg cctcttcgag accaactgaa
     2701 gatagaggtt gttctgggtg gtgcttttgc ctttgacgag gaactggcac cggttctcaa
     2761 acactgggga caacaggtta ctctgcatcg aatggtgcct cccgcacagg tcgcggcact
     2821 catgatgcag gctcagatgg cacatactgc gcctggaaat atgctttatg agctgttgac
     2881 gctcggagtg ccaagtatcg caatctctca ccatgagaaa catgctgcag tggcggagtt
     2941 cttcgccgta caaggggcaa ttgagcatct cgggatcggt gtcactctct ccgaggtcct
     3001 gatccttgag agaacgcgtt cccttcttca cgacgaagag cgccggaaga gtctccatct
     3061 ggttggaaga aagattgtgg atggcctcgg tgctgtgagg acggctgatg cgattctcaa
     3121 ggctctgcat ggagaaccca cctgattctt ctgaaaacga tttcattttc cgtgtttctt
     3181 ttgcatatcc cctatcgtta tgtgataatg tagctcgagt gtgcgggtga aatcgcttgg
     3241 ccacaccttc tgtgacggcg gcactctgag gaatgtgtga tgagcagaca ggatgatctt
     3301 gtcagaagat atggctcgat ggcgctcttt gttctgacat tgggcatcat catagcaacg
     3361 aggctgcaca gcttcctgct ctttcattct ctggcggaga tattcatcat cgccgccacc
     3421 ttggttatct gtctggtggc tctgaacaca cgtaactttc aggataaccc ctattttctt
     3481 ttcttgggaa caggactgtt ctttgtgggg ttgactcaca gtttgcatct tctggcgtat
     3541 gaaggaatga gtgttttcac gaccggtgga tcaaatctgg cggcgcaact ctggataatt
     3601 gcccgaggca ttgaaggtgt tacgatcttc ctggcacctt tcagcttgaa cttccggttt
     3661 cgtctccgtt atatcttttt cggttttctg cttctgacaa cactgttgct tgcaagcgtt
     3721 ttctactgga aagtctttcc cctctgttat gttgacggag ttggtctgac ggtcttcaag
     3781 atcgttgctg aaggagttgt ctgtgtcctg acaattcttg cttcgctggc actactgctc
     3841 aagcgcaatc tcttttgtcc gagagttctc ttccttcttc tggtttcctt cgccctgaca
     3901 ctgctggcag gagtcctttt cacactctac ataaatccat acagcgggct gaatcttctg
     3961 gggcatgtct tgacgatcgc ctcattctat cttctctatc gagccgtcgt ggaattcggt
     4021 ttgctcacac cgtacgagac catgtttcga aatctcaaac acagtgaaac agcgcttcgg
     4081 gcaagcgaag aagaactgca aacgatattc gagagcgctt cgatcatgat ggtcttggcg
     4141 gacgaacacc attgcttgcg acgtatcaac cggtcggcgg agacattctc caaaaaggcc
     4201 cgatcggatg ttctcgatgc ccgtccggga gaagcgttcg ggtgcctggg tgcattggaa
     4261 aatccgcaag gctgtggcta tggcgctctc tgtgaaacat gcattcttcg aaataccgtc
     4321 ctggacactt tcgccaaggg aacagcgcac gtacgagtgc aggcagatgt tccgttccat
     4381 ggtgctggtc gctccggaac ggtttccctg ctggtgtcca ccagcctgtt gcatatctcc
     4441 gaccgaccca tggtcttggt gtgtatggaa gacatctcac agctgagaga ggttcagcgc
     4501 gaacgagaga agatgattca cgatcttcag gaagccgcgg ctcagataaa gactctttca
     4561 ggtcttattc ccatctgcag ttct
//
LOCUS       DAOQUC010000119         4581 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_20983_length_4581_cov_3.556120, whole genome shotgun sequence.
ACCESSION   DAOQUC010000119 DAOQUC010000000
VERSION     DAOQUC010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4581
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20983_length_4581_cov_3.556120"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..758)
                     /locus_tag="PKH07_03185"
     CDS             complement(<1..758)
                     /locus_tag="PKH07_03185"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNT33982.1"
                     /translation="MMIAERLEHWAKKQEKRTALQVKRGESYDKTTYGDLWKRSLGVA
                     CLLRSSGAKPGDRVALFAGNSPEWVMAYFGIHLAGCVVVPIDAQYGNKEIETLVRFSE
                     ARAVILDRDREAVLAQISPELKGSLFLFSLDADAAYPYVFAQEAPADFQPWKHSPDDL
                     MSIIFTSGTTGEPKGVQLTCGNISSNVEGILKAVRLNKKDNILNFLPLHHAYASTAGA
                     FSPLAGGGTVTFSHSLKGTDLLATTRETGVSIFMG"
     gene            955..2796
                     /locus_tag="PKH07_03190"
     CDS             955..2796
                     /locus_tag="PKH07_03190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33983.1"
                     /translation="MRGQMMKTLAVLTLCLALVPAAMAEEEFKTTVGPVRIGEVKDTA
                     PLIVPYITWGGDMATFYANGGLTTQPNTLFQKQGLNLKLVAGDNFIQQVRDYMEGKTP
                     LLRGTMRMMGLASEVIGSDPRTKGVVILQMTWSAGDHMVVRETVKTVTDLKGKTLVLQ
                     KGGPHVGMLDDILKTAQLSWDDVKVQWAADLTGSPNCPAEIFRKDPNIAACFVITPDM
                     IGLCGGLQDTGTGAEGSVKGARVLVSTAELSRSIADVYVCRKDFYDANKDMITKFVAG
                     YLKACEEVLELKKAFEAKGSPEYMKLLQLTQDIYGKQVIPTLEEDAHGLLCDCTFVGY
                     PGNVAFFTQANNLSGFEAFQESALNLAISRGYAKVRTGFFPSGLDYTSPAFTQYLTKI
                     DVQRGERFRAEVIQDEIEALSSGGQLDDRTIVSFTISFEPNQETFSAVQYGAEYQRVV
                     EMANRYGNAVIAIRGHADPSKTLLDLVQAGTRKGILKRAGAAGNYTYSLNGKPLDLSV
                     TSTIASLIEAGEFDGVPEYNPRQTMQAALNLSKSRAETVRESIVAYAKSKGLSLDASQ
                     MQPVGVGIREPFIAKPANMEQAKQNMRVEFRLIRVTAEAATSADFDF"
     gene            2981..3910
                     /locus_tag="PKH07_03195"
     CDS             2981..3910
                     /locus_tag="PKH07_03195"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNT33984.1"
                     /translation="MKKSLVLSLSIWLAFSVVLHPFLAQTDETTGDYNNVLIVLDASG
                     SMDGTLKGTSIKKMDAAKDALRQVLQQVPADTQIGLLVFSGRNIKSDLLYPLGPRDDE
                     RLMQAISLPQPGGGTPLGAYIKTGADLLLRQRAKQRGYGTYRLLIVTDGEAGDKPLVQ
                     QYVPEVIARGITMDVIGVDMETTHTLATKAHSYRTANDPESLMRAVSEVFAEVSQAGA
                     DDTASEEAFALLDPIPAEMADAMIKALSSSGNEPIGQPRAAVAEQETIPQGHRQASPP
                     PVPKRIRTSEGIRTVFIVVAAIALIMAKFRKRR"
     gene            3945..>4581
                     /locus_tag="PKH07_03200"
     CDS             3945..>4581
                     /locus_tag="PKH07_03200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33985.1"
                     /translation="MAQKNPKGCLIAAVLWIVILGALAVAARYYILPYFKGQLEDQTG
                     SRVRYGKEIALGLDSFSGYCILRSEEMQNDLISQGIRLNICDDQADVMGRMKAFQEGR
                     LHMAVFTVDSFLTTGASLKDYPATIVMIIDETKGADAIVSYQQAVPTLDALDAPDARI
                     VLTPQSPSEFLARTVIAHFNLPKLPQQWFESAQGAEDVYQKFLKADRNARRA"
BASE COUNT         1076 a         1218 c         1276 g         1011 t
ORIGIN      
        1 cccatgaaaa tggacacccc agtctcacgc gttgtggcaa gcagatcggt ccctttcagg
       61 gagtggctga acgttactgt tccaccacca gcaagtggtg agaaagcacc agcagtactt
      121 gcgtaggcgt gatgcagcgg caggaagttc aggatgttgt ctttcttgtt caggcgaacg
      181 gctttgagaa ttccttcaac gttactggag atattgccgc aggtcagctg cacacctttg
      241 ggctctccgg tcgttccaga agtgaagatg atggacatca gatcgtcagg tgagtgcttc
      301 cagggctgga aatccgccgg tgcctcctgc gcgaagacat aggggtacgc cgcgtcggcg
      361 tccagtgaaa agagaaacag gcttcctttg agttcgggcg aaatctgtgc aagcactgct
      421 tcccgatcgc ggtcgagaat aacggctcgg gcttctgaga agcgaaccag cgtttcgatt
      481 tccttgtttc cgtactgggc atcaatggga acgaccacgc atccggccag atgaatccca
      541 aaataagcca tcacccactc agggctattt cccgcgaaga gggcaacccg gtcgcctggc
      601 ttggctcccg aagatctgag aagacaagca acaccgagac tgcgcttcca cagatcacca
      661 taggttgtct tgtcgtacga ctcccctctc ttgacctgaa gtgccgttct cttttcctgc
      721 ttcttagccc aatgctcaag ccgttcagca atcatcattg gaatcctccc tcacggggca
      781 gggtgttgcc cattattgat ttatcataga cgctcggccg gatttcgtca cgttcagaat
      841 gcatcggacg cactcggctt gatccatttg gccgtcaagc gtatatactg tatgtgaata
      901 tcaacctttg gaaagcgtat tgaatccaga agtgttgctt gaggaggcat tccgatgaga
      961 gggcagatga tgaagacgct ggccgttctg acattgtgtc tggctttggt gcccgccgcc
     1021 atggcggaag aagagttcaa gaccaccgtg ggtcctgttc gcattgggga ggtcaaagac
     1081 accgccccgc tcatcgtgcc ctacatcacc tggggcggtg acatggcgac gttctacgcc
     1141 aatggcggtt tgaccacaca gcccaacacc ctcttccaga agcagggact gaatctcaaa
     1201 ctggttgccg gtgacaattt catccagcag gtacgtgatt acatggaagg caagacacca
     1261 ttgcttcgag gaaccatgcg catgatgggc ctggcttctg aagtgatcgg ttccgacccc
     1321 agaacgaaag gggtcgtcat cctccagatg acctggtctg ctggcgacca catggtcgtg
     1381 cgcgaaaccg ttaagactgt taccgatctc aaaggtaaga cccttgtcct tcagaagggc
     1441 ggaccccatg tcgggatgtt ggatgatatc ctcaagaccg cccaactcag ttgggatgat
     1501 gttaaggttc agtgggcggc agacctgacg ggatcgccca actgccctgc agaaatcttc
     1561 cgaaaagatc caaacatcgc cgcttgcttt gtgatcacgc ccgacatgat cggtctgtgc
     1621 ggtggcttgc aggacacagg cacaggcgcg gaaggctctg tcaaaggtgc gagagttctg
     1681 gtctcaactg ctgagttgtc gcgctccatc gcagacgttt atgtctgtcg caaggatttc
     1741 tatgatgcca acaaggacat gatcacgaaa ttcgttgccg gctatctgaa agcctgcgag
     1801 gaagtactcg agctgaaaaa ggcctttgaa gccaaaggct ctcccgaata catgaagctt
     1861 ttgcagctga cacaggatat ctatggcaag caagtcattc caacactcga agaagacgca
     1921 cacgggctgc tttgcgactg tacttttgtc ggctatccgg gcaacgtcgc cttcttcacg
     1981 caggccaaca acctgagtgg gttcgaggcg tttcaggagt ccgctttgaa tctcgcgatc
     2041 agccgtggtt atgcgaaagt ccgaacaggt ttctttccat cgggcttgga ctacacgtca
     2101 cctgcgttta ctcaatatct gaccaagata gacgtccaac gtggagagcg cttccgtgcc
     2161 gaagttattc aggatgagat cgaagcactg agttctggcg gacagctcga cgaccggacg
     2221 atcgtctcgt tcaccatcag ttttgaaccc aaccaggaaa ccttcagcgc agtgcagtac
     2281 ggcgctgagt atcaacgagt cgtcgaaatg gcaaacagat atggcaatgc cgtcatcgcc
     2341 atccgaggac acgccgatcc gagcaagacc cttctggatc tcgttcaggc tggcacccga
     2401 aagggaatac tcaagcgcgc tggcgcggca ggcaactaca cttattccct gaatggcaag
     2461 cctttggatc tgtccgtgac gagcacgatc gcttccttga tcgaggcagg cgagtttgat
     2521 ggagtgccgg agtacaatcc gcgtcagaca atgcaggccg cgttgaacct ctccaagagc
     2581 cgtgctgaga ctgtccgtga atccatcgtt gcctatgcca agagcaaggg actgagcctg
     2641 gacgcaagcc agatgcagcc tgtgggggtt ggcatccggg agccgttcat cgccaaaccg
     2701 gcgaacatgg agcaggccaa acagaatatg cgcgtcgagt ttcgtctcat tcgcgtaacg
     2761 gcagaggcgg cgacctctgc ggactttgac ttctaacgag tcaggacttg ttgctggcgt
     2821 atgggcggtg tcacacgaac gagaggttgc cgttaaagat tggagttcgg ttattggaag
     2881 atgcggatct gcaacgagtt tgtccgggca ggctgtctgc atcaaaaccg agaaagtagt
     2941 gctgggtcta tcccgcgtcg gatcgctggg gaggaacaag atgaaaaaat cgttggttct
     3001 gagtctttcg atttggctcg cattttctgt tgtcttgcac ccgtttctcg cacagacgga
     3061 tgaaacaact ggggattaca acaatgttct cattgttctg gatgcgtcag gctccatgga
     3121 tggaacgctc aagggaacat ccatcaagaa gatggacgca gccaaagatg cacttcggca
     3181 ggtgcttcag caggttccgg cggacactca gatcggattg ctggtgttca gcggtcgcaa
     3241 catcaagagt gatctgctct atccactcgg gccgcgagac gacgaacgac tgatgcaggc
     3301 tatctcgctt ccccaacccg gtggtggaac acctctggga gcctatatca agaccggtgc
     3361 cgatctcctt ctccggcaac gagccaagca gcgaggctat ggaacatatc ggctcctgat
     3421 cgtgaccgat ggagaagcgg gtgacaagcc gttggtacaa cagtacgttc ctgaagtgat
     3481 cgcccgtggg atcacgatgg acgtcatcgg tgtggacatg gagacaactc ataccttggc
     3541 aaccaaggcg cattcttatc gcactgccaa tgacccggaa tccctcatgc gagccgtctc
     3601 ggaagtcttt gctgaggtca gccaggcagg tgccgatgac acagcaagcg aggaagcctt
     3661 tgcacttctg gatccaattc ctgcggaaat ggcggacgca atgatcaaag cgctgtcctc
     3721 aagcggcaat gagccgatcg gccagccccg tgcggcagtc gcagagcaag aaaccatccc
     3781 acaaggacac agacaggcat cgccaccacc tgttccgaaa cggatacgta cctccgaagg
     3841 gatacgaacc gtcttcatcg tggttgccgc aattgctctg attatggcaa aattccgaaa
     3901 acgcaggtga tccgcttgtt tgcgagagga aggtaagaag cacaatggca cagaagaacc
     3961 ccaaaggatg cctcatcgct gcggtgcttt ggattgtcat ccttggagcg ttggcagtcg
     4021 cggcgcgcta ctacatactt ccctatttca agggacagct ggaagatcag acgggatccc
     4081 gcgtgcgcta tggtaaggag attgcactcg ggctcgattc cttctccggc tactgcatcc
     4141 tgcggtctga ggagatgcag aatgacctga tcagtcaggg aattcgactg aacatctgtg
     4201 acgatcaggc cgacgtgatg ggcagaatga aagcatttca ggaaggaaga ctgcacatgg
     4261 cagtctttac cgtggattcc tttctgacca caggagcctc tctgaaggac tatcctgcca
     4321 caatagtcat gatcattgat gagaccaaag gtgcggacgc cattgtttct tatcagcagg
     4381 cggttccgac gcttgatgcc ctggatgcac cggatgcgcg aatcgtgctc acgccacagt
     4441 ctcccagtga gtttcttgcg cggacggtca ttgcacattt caatctgcct aagcttcctc
     4501 agcagtggtt tgagtccgct cagggagcgg aagatgtcta tcagaagttt ctcaaagcag
     4561 acaggaacgc ccgacgagcg t
//
LOCUS       DAOQUC010000120         4580 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_20992_length_4580_cov_3.586519, whole genome shotgun sequence.
ACCESSION   DAOQUC010000120 DAOQUC010000000
VERSION     DAOQUC010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4580
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20992_length_4580_cov_3.586519"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1089
                     /locus_tag="PKH07_03205"
     CDS             <1..1089
                     /locus_tag="PKH07_03205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33986.1"
                     /translation="PGAGGTYFGYDPGVTVFIAGAQDLSTALPAPMTSAVRDFLVGES
                     YLAFKERRFSHARLEYMTNRSFHDIDGDSGNDVDLVASSASSVETALDAVVAYAGSTA
                     PVTLFLVGKLGSGTSLLMPDNSTLTASQLDTWLDAKFGSSRDIRILVEASNSRAFCEA
                     LKDRNRVVVSSAPDSNASFLCDGALSFLNGYATYTRKNLPMLQCVEMTKNVLTYGCYY
                     GLSLPQAFVGEDVEPDLLYHGLPKDAADMLPPDIEGVFEPMVVGRSECPVEIFAFACD
                     DSALNQVSALIIKPDGNSATTCLDWDGVSGSRKYRTWLDSDVLTKAGTYVIAVYATDA
                     VGNVSQPKISQIGISEPSTAVRYWEQME"
     gene            complement(1199..1564)
                     /locus_tag="PKH07_03210"
     CDS             complement(1199..1564)
                     /locus_tag="PKH07_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015178216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNT33987.1"
                     /translation="MLLDSNIIIYASKEENSHLDSFLQHGDFCVSVISRIEVLGYHKL
                     SVREVLWLESLFDSLSCLPLDEPVAGLAILLHQKQKISLADAIIAATALHHNLPLVTR
                     NIADFQSIPNLQLIDPFKQ"
     gene            complement(1567..1836)
                     /locus_tag="PKH07_03215"
     CDS             complement(1567..1836)
                     /locus_tag="PKH07_03215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33988.1"
                     /translation="MKLCSIKSAPEQIRTLVELVTTGEEVLLTNDDGPIARIVPIAGH
                     GQNISKGTNLVEIFKQIRALGGVSIEDPVAWQKETRQDRSLPGRD"
     gene            complement(1954..3813)
                     /locus_tag="PKH07_03220"
     CDS             complement(1954..3813)
                     /locus_tag="PKH07_03220"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNT33989.1"
                     /translation="MGRSRTSIGIVAFVLMLGCASSGVNQSDFVAPWVSEGDKALPWT
                     KAEFRNSPDNFQFVVVSDRNGGHRPRIFEDAVSKINILQPEFVICVGDLIPGYTKSEA
                     RVSAQRKEFIGIAEKLEMPFFYVPGNHDITNPMMSEDWHKRFGRSYYHFLYKDVLFLC
                     LNSEDPPADHISQQQVEYVAQALKENRNVRWTFVFIHKPLWDRKGNHNWDKVEALLGD
                     RKHTVFSGHTHRYLMRERNGHDYLTLGVTGGGSALRGVEYGEFDHFLWVTMTEKEPKI
                     ANLMLEGVWDKNTLTEERTTMLPSLNRNELFHLEPLWSPGEAFTQDKTVLTISNPFDH
                     RLSVRMNLAEHPSIVSETKELAVTIPPKEVKEIPIIFKASQPPDFLNTPKVLLSWTGA
                     FHFEGKRELKGQGKLPLFLDTIYKIPVRKVPVTVDGILDEWNSLPFICDNPLEFALRA
                     QEWKGAADSSFRFTVERDLYHLYVAVHIFDESLGLAPGKLPWEQDGVEIRIDARADPE
                     RSEGIGENQLVDFLLFAMSPTAPDQSPLIAFEDQIPEGMKYACAPTSDGFSAEVAVPI
                     SYLNEKQNGSWEQVRINIAVTDFDDELATGSQIWWRPDWRKARNYKGSGTFEK"
     gene            complement(3916..>4580)
                     /locus_tag="PKH07_03225"
     CDS             complement(3916..>4580)
                     /locus_tag="PKH07_03225"
                     /inference="COORDINATES: protein motif:HMM:NF014097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF128 domain-containing protein"
                     /protein_id="HNT33990.1"
                     /translation="ISTIPIDRAARAVGEMMQVFRAGLSMGQLATFGLPGEKIGGFTV
                     PDGNIAVGTVCSVTINGIFLSEGIATCSRFGGLLEIDQGRPTRFTQIIHYDGTTLDPL
                     EIFIKGHMTRVREASSGGKGVIGASFREIPAPALPAVTKLDRRLQKIGLGAVLLVGKP
                     NQPVLDIPVQNGRVGIVVIGGLNPIAAVEEAGIETKNMAMAVLHDFENLVPCSDMLKI
                     KF"
BASE COUNT         1045 a         1209 c         1220 g         1106 t
ORIGIN      
        1 cctggagcgg gcggaacata cttcggatat gaccctggag tgacggtgtt catcgccgga
       61 gcgcaggatc tttcaacggc gctgcccgca ccgatgacgt cggcagttcg ggatttcctg
      121 gtgggtgagt cgtatcttgc attcaaggag agacgcttca gccatgcgcg actggagtac
      181 atgaccaacc gaagtttcca tgacattgac ggggattcgg gcaatgacgt ggatctggtc
      241 gccagttctg catcatcggt tgagacagcg ctggatgctg ttgttgccta tgcgggatca
      301 acagcaccag tgacgctgtt cctcgtcggt aagttgggca gtggcaccag ccttctgatg
      361 cctgataatt cgacgctgac agcaagtcag ctggacactt ggttggatgc caaattcgga
      421 tcgagtcggg atattcgaat tctcgtcgag gcatcgaact ccagagcgtt ttgcgaggca
      481 ttgaaggaca ggaatcgagt ggtcgtctcc agcgctccgg actccaatgc ctcattcctg
      541 tgcgatggtg ccctgtcatt tctcaatggg tatgccacgt acacccgaaa gaacctgccg
      601 atgttgcagt gcgtcgagat gacgaaaaac gtcctcacct atggctgcta ctacgggctc
      661 tcactcccgc aggcgtttgt tggagaggat gtggagccgg atctgctcta tcacgggctt
      721 ccgaaggatg cagcggatat gctgccgcct gacattgagg gcgtgtttga gccgatggtg
      781 gtgggtcgca gtgagtgtcc ggttgagatt ttcgcgtttg cctgtgacga ctcagcgctg
      841 aatcaggttt ctgctctgat catcaagccg gatggcaact cggcaacaac gtgtctggac
      901 tgggatggtg tttccggcag tcggaagtac cggacctggc ttgactcgga tgtgctcacc
      961 aaagcgggaa cctacgttat tgccgtgtat gccaccgacg cggttggaaa cgtctctcaa
     1021 ccaaagattt ctcagatcgg catttccgaa cccagcacgg cagtgcggta ctgggagcag
     1081 atggagtaac ggtgcgaccc gcttctgcac ggtctgatgt ctcgatgcca gagacgagtt
     1141 tgtctctatg cgccgcccca gctcagattt ggggtcaacg gcctccaaac gaaccagtct
     1201 actgcttgaa tgggtctatc agctgcagat tcggaatgct ctgaaaatca gcgatattcc
     1261 gggtgacgag agggagattg tgatgaagtg ctgttgcggc aatgatcgca tcagccaaag
     1321 agatcttctg tttctggtga aggagaatgg cgaggccggc aaccggttca tcgagtggta
     1381 gacaggacag actgtcgaag agactctcaa gccagaggac ttctctgacg gaaagtttgt
     1441 gatatcccaa aacctcgatc cggcttatca cagagacgca gaaatcaccg tgctgcagga
     1501 aggaatcaag gtgcgagttc tcctctttgg aagcgtagat gatgatattg ctgtccagaa
     1561 gcatgattag tcccttcccg ggagtgaccg gtcttggcga gtctcctttt gccaagcgac
     1621 gggatcttca atgctgactc cccccagagc gcgaatctgc ttgaatatct ccacgaggtt
     1681 cgttcctttt gagatattct gtccgtgtcc agcaatggga acgattcgag cgattgggcc
     1741 atcgtcattg gtgagcagca cttcttcgcc cgtggttacc aactcaacaa gggtacgaat
     1801 ctgttcgggt gcgcttttga ttgaacagag tttcacagca agtcacctcc ttgaaatacc
     1861 caaatgagta tagcacattc cgacaaaacg tgagaaggtt ccgaacgggc taacctatac
     1921 caagcgaaag tatgcataca ccacttttca aatctatttc tcaaacgtcc cggatccctt
     1981 gtagttacgg gctttccgcc aatcgggacg ccaccagatc tgtgagcctg ttgccagctc
     2041 gtcatcgaaa tctgtcacgg caatgttgat tcggacttgc tcccaggagc cgttctgctt
     2101 ttcattcaga taggaaatcg gaacggcgac ttccgcgctg aaaccatccg atgtgggagc
     2161 acaggcgtat ttcatgccct cggggatttg gtcttcaaag gcgatcaggg gagactgatc
     2221 tggcgctgtt ggactcatgg caaagagaag gaaatcaacc aactggttct cgccgatacc
     2281 ttcggagcgt tcgggatcgg ctctggcatc aattcgaatt tcgacgccat cctgttccca
     2341 ggggagtttt cctggcgcca acccaagtga ctcatcaaag atatgcacgg caacatagag
     2401 gtggtacaga tcacgctcca ccgtgaaccg gaaactgcta tccgcagccc ctttccattc
     2461 ctgtgctctc aatgcgaatt cgagcgggtt gtcgcagatg aaaggaaggc tgttccactc
     2521 atctagaatg ccgtccacgg tgaccggtac tttgcgcacc ggaatcttgt atatcgtatc
     2581 aaggaataga ggcaactttc cctgtccctt cagctcacgc tttccctcaa aatgaaatgc
     2641 tcctgtccaa ctcagcagaa ccttcggagt attgaggaaa tccggtggct gagatgcctt
     2701 gaagatgatc gggatttctt tgacttcctt tggtggtatc gtgactgcaa gttccttggt
     2761 ttcggacaca atgcttggat gttccgccag attcatccga acagacaggc gatgatcaaa
     2821 cggattcgaa atcgtaagaa ccgtcttgtc ctgggtgaag gcttctccag gcgaccagag
     2881 cggttccagg tgaaacaact cattgcggtt cagcgaagga agcattgttg ttctctcttc
     2941 tgtgagagtg ttcttgtccc agacgccttc gagcatcaag ttggcgatct tcggttcctt
     3001 ctctgtcatg gtcacccaca gaaagtggtc aaattcaccg tactcaacgc cgcgaagtgc
     3061 acttccacca ccggtgactc ccagcgtgag atagtcatga ccgtttctct cgcgcatgag
     3121 ataacggtgc gtgtggccgg agaagaccgt gtgctttcgg tcgccaagga gtgcttcaac
     3181 cttgtcccag ttgtggtttc cttttctgtc ccaaagcggt ttgtggatga acacaaaagt
     3241 ccagcgcaca tttcgattct ctttgagcgc ttgagcaaca tactccacct gctgttggct
     3301 gatatgatcg gcggggggat cctcactgtt cagacagagg aacaggacat ctttatacag
     3361 gaaatgatag taggagcgcc caaagcgctt gtgccagtct tcgctcatca tgggattggt
     3421 gatgtcatgg ttgccgggaa catagaagaa aggcatttcc agcttctcag cgattccgat
     3481 gaactccttg cgttgggcgg agactcttgc ctcgctcttg gtgtatcccg ggatcaagtc
     3541 cccaacacag ataacaaatt ccggttgcag gatattgatc ttgctgaccg cgtcttcgaa
     3601 tatccttggc cggtggccac cgtttctgtc agaaaccaca acgaactgga aattatccgg
     3661 actattccgg aactcagcct ttgtccaggg cagagccttg tctccttctg aaacccatgg
     3721 ggcgacaaaa tcagactgat tgaccccgga cgaggcacac ccaagcatca acacaaatgc
     3781 gacaatccca atactcgtac gacttcgacc catgacacct cctcgtagac tgcctgacag
     3841 tgcggtaccg tctctgattc tgaagtgctg agttgcaggg cggaaatgcc ccaaccactc
     3901 ggcaacattg accgcctaga atttgatttt gagcatatca gaacagggca ccagattctc
     3961 aaaatcatga agcacagcca ttgccatatt tttggtctca attcctgctt cttcgaccgc
     4021 tgcaatggga ttcagcccac cgatgaccac gattccgacg cggccgttct gaactggaat
     4081 atccagaacc ggctgattgg gcttgccaac caacaggact gcccccagcc caatcttctg
     4141 gagtcttcgg tcaagttttg tcaccgctgg cagagcgggt gccggaatct cccgaaaact
     4201 ggcgccgatc acgcccttgc caccagaaga ggcttcccga acgcgggtca tgtgaccctt
     4261 gatgaagatt tccagcggat cgagtgttgt tccgtcataa tggatgattt gggtaaagcg
     4321 ggtcggacgt ccttgatcga tttccaggag ccctccaaaa cgggaacacg tggcaattcc
     4381 ttcggacaga aaaatgccat tgatggtgac gctgcagacc gttccaacgg caatattgcc
     4441 gtcaggaacg gtaaatccac cgatcttctc tccaggtagg ccaaacgttg ccaattgtcc
     4501 catgctcaag cccgctcgaa agacctgcat catctctccg acagccctcg ccgctcggtc
     4561 aatgggtatc gtggagatgt
//
LOCUS       DAOQUC010000121         4569 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_21065_length_4569_cov_2.734160, whole genome shotgun sequence.
ACCESSION   DAOQUC010000121 DAOQUC010000000
VERSION     DAOQUC010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4569)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4569)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4569
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21065_length_4569_cov_2.734160"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..321
                     /locus_tag="PKH07_03230"
     CDS             <1..321
                     /locus_tag="PKH07_03230"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNT33991.1"
                     /translation="KEIAGDIAEGKRTLILIYLLQRISAEQRRRVDQVYRLPREQKTP
                     EQIAEILQWMKETGSVESASQRSRELAREALGLFQKAFALVPDSQAKNDLQEAFDFVI
                     SRQL"
     gene            386..1567
                     /locus_tag="PKH07_03235"
     CDS             386..1567
                     /locus_tag="PKH07_03235"
                     /inference="COORDINATES: protein motif:HMM:NF017495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HNT33992.1"
                     /translation="MNKAVLIEDSVLLFVYFTGVVGFIFWVSSLQSFKGLFRFLPPLV
                     WAYFLPMIGTTFSITPADSTLYGFLRKVLLPFLMVLLLLSTDIKAIWRLGPLAIATML
                     FGSVGVVVGAVISFSIFKGFLPAESWKGIAALSGSWIGGSANMAAIAESVKASPDLYA
                     PLIVVDTVVGYAWLGILLALVGQQDRIDLKMRAKTAILHSIAQHLAAEDGKMRRPLTV
                     KDFTAIVAVGFVVSTLAMKLGGVLPEVSNVISTFTWGILILTFLGIVLSFTRLRQLEF
                     AGAGSIGYGCLYILLASIGAQADLRAVINTPQYILLGVVWMGIHAVLLFVGAWILRSP
                     MFLVATGSMANIGGTSSGPVVAAAYQPSLAPVGLLLAVLGGVIGTPLGILVAWICSKL
                     A"
     gene            1614..2696
                     /locus_tag="PKH07_03240"
     CDS             1614..2696
                     /locus_tag="PKH07_03240"
                     /inference="COORDINATES: protein motif:HMM:NF019375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1611 domain-containing protein"
                     /protein_id="HNT33993.1"
                     /translation="MVYHPGKKLVILAEGCFGKKGSKTAIGVIRYGANPVAAVLDSSH
                     AGQTVADVLGIRPDIPIVSSLDEVESLLGTTADALVIGIAPRGGSLPAAWKDIIKDAL
                     DRGMDVWNGLHLMMTEDPELMEHAQRSGAVVWDVRKPQPNLPVSSGHARHVPAHVILA
                     VGSDCSVGKMTDMLEVEKVATQRGIRALFCPTGQTGIMIKGWGVPIDRVISDFVAGGA
                     EKLVLEAAEDNDLILVEGQGSLFHAGYSGVTLGLMHGTMPDHLVLCYQVGRPTVGDGY
                     GVNTPSLKEMIRVYETMAAAVKPSPVIAIVVNTWGMSDEEARAELKRAEAETGLPSDD
                     VVRYGADRILDAILEAGKTLSKPART"
     gene            2723..3859
                     /gene="ald"
                     /locus_tag="PKH07_03245"
     CDS             2723..3859
                     /gene="ald"
                     /locus_tag="PKH07_03245"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012174559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HNT33994.1"
                     /translation="MIVGVPKEIKDNEYRVGMVPSGTEKLVNDGHKVLVQKDAGVGSW
                     ISDEEYRATGATIVDTAAEVFAKADMIVKVKEPQPSELDMLRKEQILFTYLHLAADRE
                     LTERMLKTKAVGIAYETVTSPDGSLPLLTPMSEVAGRISIQEGAKYLEKTFGGRGVLL
                     SGVPGVKPASVVILGAGVVGTNAAKVAAGLGASVTVLDINLDRLRYLDDIMPPNVMMV
                     KSDRLNILKALKDADMVVGAVLVRGARAPRLITRDMLKQMKTGAVIVDVAVDQGGCCE
                     IVRPTTHSDPVYVEEGVVHYCVTNMPGVVSRTSTFALTNATIEYAVEIANKGYRNAVL
                     QNPALYAGLSVWCGHLTCSAVAESFGMKFEEARSLLCVDSRQYQ"
     gene            4112..>4569
                     /locus_tag="PKH07_03250"
     CDS             4112..>4569
                     /locus_tag="PKH07_03250"
                     /inference="COORDINATES: protein motif:HMM:NF012885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT33995.1"
                     /translation="MGIARTPAGADLLGRLFVKALRYLRGKAIQYAVNPSSKVFMADT
                     TLRDGEQSPGASLDIRSKLRIAHALADAGLDIIDAGFPACSPGEEEAVRQIATEVRNP
                     TITCLSRVKKEDIDASARALKPAKQCGITLFLGIPFSSLFTRRRCQGDVP"
BASE COUNT          993 a         1127 c         1365 g         1084 t
ORIGIN      
        1 aaggagatcg cgggcgacat cgctgagggc aagcgcaccc tgattctcat ctatctgctc
       61 caacgcattt ccgccgaaca gcggcgcagg gtggatcagg tctaccgact tccgcgagag
      121 caaaagacgc cggaacaaat cgcggaaatc ctccagtgga tgaaggagac agggagtgtc
      181 gaatctgcct cccagcgttc gcgggaactg gcacgcgaag cgctcggact gtttcagaag
      241 gcctttgccc tcgtgccgga cagccaggcc aagaacgact tgcaggaagc ctttgacttt
      301 gtcatatcga ggcaactcta gagttcccat ggagtgggcc tgtcgtgaag tgatcaagaa
      361 cgttgtaagt tgagcgtgag ggtttatgaa taaggctgtg ttgatagagg attccgtcct
      421 tctgtttgtc tatttcactg gtgtcgttgg ctttattttc tgggtcagtt ctctgcagtc
      481 gttcaagggt ttgttccgct ttctgcctcc cttggtttgg gcttattttc ttccgatgat
      541 tgggacaaca ttctcgatca ctcccgccga cagtaccctc tacggttttc tcaggaaggt
      601 gctcttgcct tttctgatgg tcttactcct tctctccaca gacatcaaag ccatctggag
      661 actcgggcct ttggcaattg caacgatgct gtttggctct gtgggagttg tcgtcggcgc
      721 tgtgatctcg ttctcgatct tcaaaggctt tctcccggct gaaagttgga aaggtattgc
      781 agcgctatcg ggaagttgga tcggtggcag cgccaacatg gccgccatcg ccgaaagcgt
      841 taaagcctct cccgatctct atgccccttt gattgtggtg gatactgttg tcggctatgc
      901 gtggctcgga atactgctgg cgctggtcgg tcaacaggat cgcattgatc tgaaaatgag
      961 agccaaaaca gccattttgc actccatcgc tcagcacctt gccgccgaag atggaaagat
     1021 gcgtagacct cttacggtga aggatttcac cgcaatcgtg gcggtaggtt ttgtggtaag
     1081 taccttggcc atgaaactgg ggggagtact tcctgaagtg agcaatgtga tctcgacgtt
     1141 cacttggggt atcctgattc tgaccttttt ggggattgtc ctgtccttca cacgactgcg
     1201 gcaattggag tttgccggcg ccggatcaat cggctatggg tgcttgtaca ttctgttggc
     1261 atcaatcggt gctcaagctg atctacgggc agtcatcaac acccctcaat atatcttgct
     1321 gggagtcgtg tggatgggca ttcacgcggt gctcttgttt gttggagcat ggatacttcg
     1381 ctcgccgatg tttcttgtgg caaccggcag tatggcaaat atcggaggta cgagctcggg
     1441 acctgttgtg gcagcggcgt atcaaccatc tctggcacca gtcggactgc tattggccgt
     1501 actgggggga gtcatcggga caccgctggg aattctggtt gcctggatct gctcaaaact
     1561 ggcatagtat gcaaaccgaa tggagataca ctacaaaaca gaggcggtaa tagatggtct
     1621 atcatcctgg aaagaaactg gtcattctcg ctgaaggctg ctttggcaag aagggttcaa
     1681 agacggctat tggtgtgatt cgctacggtg ccaacccggt ggctgcagtg ctggacagtt
     1741 cacacgccgg ccaaacggtt gcagacgttc tcgggattcg cccggatatt cctatcgtgt
     1801 cttcccttga cgaggtggag tcgctcttgg gaacaacggc agatgctctg gtcattggga
     1861 tcgcgccacg aggaggctct ctgcctgctg cctggaaaga tatcatcaag gatgccctgg
     1921 atcgcggcat ggatgtctgg aacggtcttc acctgatgat gaccgaggat cctgaactga
     1981 tggagcatgc ccaacgttcc ggcgcagtcg tctgggatgt gcgaaagccc cagccgaatc
     2041 tgccggtctc cagcggacat gctcggcatg tgcccgccca cgtcattctc gcagtgggga
     2101 gcgattgctc ggtgggaaag atgaccgaca tgctggaagt cgaaaaggtg gccacccaac
     2161 gaggcatccg agctcttttc tgtcccacag gacagactgg aatcatgatc aaaggctggg
     2221 gcgttcccat tgatcgagtc atctcggatt ttgttgcggg tggagccgag aaactcgttc
     2281 tcgaagccgc cgaggacaat gatctgattc ttgttgaggg acaaggctcc cttttccacg
     2341 caggctattc tggtgtgaca ctcggattga tgcacggaac gatgcctgac catctggtgt
     2401 tgtgctacca ggtcggaagg ccaacggtgg gggatggcta tggagtgaat acgccatcgc
     2461 tgaaggagat gattcgagtt tatgaaacca tggctgcggc ggtcaaaccc tctccagtga
     2521 tcgccatcgt cgtcaacaca tgggggatgt cggacgaaga ggcacgagcg gaactgaaaa
     2581 gggcggaagc cgagacgggt ttgccaagtg atgacgtggt gcgatatggt gctgacagaa
     2641 tcttagatgc tattctggaa gctggaaaga cgttgtcgaa gcctgccaga acgtaggcgg
     2701 cttgctgcga aaggagcaaa tgatgatcgt cggagtgccc aaagagatta aggataatga
     2761 gtatcgagtc ggcatggttc catcaggaac agagaaattg gtcaatgatg gccacaaggt
     2821 gttggtgcag aaggatgcgg gagtggggag ctggatttcc gatgaggaat accgtgccac
     2881 gggagcaact attgttgata ctgcggcgga agttttcgcc aaagcggaca tgatcgtcaa
     2941 ggtcaaggag cctcagccat cggaactgga catgcttcgc aaggagcaga ttctttttac
     3001 ctaccttcat ttggccgctg atcgcgagct gacggaacga atgctcaaga ctaaggctgt
     3061 cgggatcgct tacgaaactg taaccagtcc ggacggctcg ctgccgctgt tgacaccgat
     3121 gagtgaggtt gctggacgaa tctcgattca ggaaggcgcc aagtatctgg aaaagacctt
     3181 tggcggccgt ggagtgctcc tcagtggcgt gcccggcgtg aagccggcca gtgttgttat
     3241 tttgggtgcc ggcgtcgttg gaaccaatgc tgcaaaggtt gctgccgggc ttggcgcatc
     3301 ggtgacagtg ctcgatatca acctggacag actccgatat ctcgatgaca tcatgccacc
     3361 gaatgtgatg atggtgaagt cggatcgtct caatatcctg aaggcgctca aggatgcaga
     3421 catggtcgtg ggcgcggttc tggttcgtgg cgcgcgcgct ccaaggttga tcacccgcga
     3481 catgctgaaa cagatgaaga cgggagcggt catcgttgat gttgccgtgg atcaaggcgg
     3541 ctgctgcgaa atcgttcgac cgaccaccca tagcgatccg gtctacgttg aagaaggcgt
     3601 cgttcactac tgcgtgacca acatgcctgg cgttgtcagc cgaacttcaa ctttcgctct
     3661 gaccaatgcc accatcgagt acgcagtcga gatcgccaac aaaggatacc gcaatgcggt
     3721 tcttcagaat cccgccctgt atgccggtct gagcgtctgg tgcggacatc tgacctgttc
     3781 cgccgttgca gagtctttcg gtatgaagtt tgaggaagcg cgatcgcttt tgtgcgtgga
     3841 ctctcgccag tatcaataag atcttttggt gacacagagt ccgagagggg catgtgattt
     3901 gttgagcgag cgtccaagag agtgggcata gcccacacgc cagcaggtgc caacctgctt
     3961 ttggtgacac aaagtccgag aggggcatgt catttattga gtgagcgtcc aagggagtgg
     4021 gcatagccca cgcgcccgca ggtgctaacc tgctcttggt gacacagagt ccgagaggga
     4081 tgtgtcattt gttgagtgag cgtccaaggg aatgggcata gcccgcacgc ccgcaggtgc
     4141 tgacctgctc gggagattgt ttgtgaaggc actccgatac ctcagaggaa aagcgatcca
     4201 gtatgccgtc aatccgtcat ccaaggtgtt catggcggac acgacgctac gcgatggaga
     4261 acagtcccct ggcgccagtc tggacatcag atcaaagctg cgcatagcgc atgcccttgc
     4321 tgatgccggt ctggacatca ttgatgcggg ttttcccgct tgttcgcctg gtgaggaaga
     4381 agcggttcgc caaatcgcga ctgaggtcag aaatccgacc ataacctgtc tttcgcgtgt
     4441 gaagaaagaa gacattgacg cttcagctcg agcgcttaaa ccggcgaaac aatgtggcat
     4501 tacgctcttt cttgggatac ccttctcatc tttgttcacg cgacgacgat gccagggaga
     4561 tgtcccaag
//
LOCUS       DAOQUC010000122         4565 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_21090_length_4565_cov_3.435920, whole genome shotgun sequence.
ACCESSION   DAOQUC010000122 DAOQUC010000000
VERSION     DAOQUC010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4565
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21090_length_4565_cov_3.435920"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            86..235
                     /gene="rpmG"
                     /locus_tag="PKH07_03255"
     CDS             86..235
                     /gene="rpmG"
                     /locus_tag="PKH07_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008401093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HNT33996.1"
                     /translation="MRERITLACTDCKQRNYNTTKDKRKQTARFEIKKYCRFCRKHTA
                     HRETR"
     gene            275..347
                     /locus_tag="PKH07_03260"
     tRNA            275..347
                     /locus_tag="PKH07_03260"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:308..310,aa:Trp,seq:cca)
     gene            567..791
                     /gene="secE"
                     /locus_tag="PKH07_03265"
     CDS             567..791
                     /gene="secE"
                     /locus_tag="PKH07_03265"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HNT33997.1"
                     /translation="MIAKMNATLKDWYTKTVAYIKDVRRELNFVTWPTRQEIKDSTKI
                     VFVTVIVIAIYIAVLDGVFTNVFKFIFTRF"
     gene            898..1521
                     /gene="nusG"
                     /locus_tag="PKH07_03270"
     CDS             898..1521
                     /gene="nusG"
                     /locus_tag="PKH07_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HNT33998.1"
                     /translation="MNENHEQEPLENQGQAPDQVGADPSETPVNPAASWYAVHTYSGY
                     ENRVRESLLNLVRSDFRLKDKIIEVLIPTEEVAEIREGKKRVSSKKLFPGYVVVHMEM
                     SDELQTLVRSTPGVTGFVGPRRTPVPLTTDEVDHILGRMKETEETPRPKVVFECGEKV
                     KVIEGPFESFTGYVSEINQDRGRVTVMVDILGRSTPVELDFLQVDKL"
     gene            1620..1754
                     /locus_tag="PKH07_03275"
                     /pseudo
     CDS             1620..1754
                     /locus_tag="PKH07_03275"
                     /inference="COORDINATES: protein motif:HMM:NF024183.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
     gene            1749..2048
                     /locus_tag="PKH07_03280"
     CDS             1749..2048
                     /locus_tag="PKH07_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006854031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NINE protein"
                     /protein_id="HNT33999.1"
                     /translation="MCTKCGVSLASTLSLGPKNKLVAGLLGILLGSLGIHKFYLGYNK
                     EGIIMLVVSVAGGMVTCGAGSVVVGIIGLIEGILYLTKSDEEFDRVYVQGTKTWF"
     gene            2114..2545
                     /gene="rplK"
                     /locus_tag="PKH07_03285"
     CDS             2114..2545
                     /gene="rplK"
                     /locus_tag="PKH07_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HNT34000.1"
                     /translation="MPPKKKVQAIVKLQCPAGAANPAPPVGPALGQHGVNIMEFCRQF
                     NERTKTESGMIIPAVIMVYADKSFTFITKTPPAAILLKKAAGIAKASGQPNKNKIGKV
                     SRKQVEEIAKTKMEDLNAFDMEAAVKMVEGTARSMGIEVEA"
     gene            2542..3237
                     /gene="rplA"
                     /locus_tag="PKH07_03290"
     CDS             2542..3237
                     /gene="rplA"
                     /locus_tag="PKH07_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007502214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HNT34001.1"
                     /translation="MIKSKRLVDAHKTFDRAKLYTLPEAVKLVKQSAKAKFDETIELS
                     AKMGVDPRKADQQIRGTVQLPHGTGKQLRVVVFAEGALAEEAKQAGADYVGSDDLTQK
                     IAGGWVDFDIALATPDMMRNVGKLGKVLGPRGLMPNPKAGTVSAKIGEAVQEFKAGKI
                     EYRLDKQAGIHVPVGKASFAPNKLEENIRSLVSALMRAKPSTAKGQYLKGLALSSTMG
                     IGVKLDLSDFRDA"
     gene            3466..3996
                     /gene="rplJ"
                     /locus_tag="PKH07_03295"
     CDS             3466..3996
                     /gene="rplJ"
                     /locus_tag="PKH07_03295"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HNT34002.1"
                     /translation="MLKKQQKAEIVDSLVEQLKDVECVFLVDYRGLKVEEVFQLRTAL
                     RKTDSRMRVLRNTLLTRVFKRSQNEKAGEMVEGPTAVIWTKGDIVEAAKALDAFAKEH
                     ENLKIKFGILENKIITDKSIAALAKLPPKQVLLGQLVGVLEGPISGLVFALEGVISEL
                     VYTLQAVVDKREQTAA"
     gene            4050..4430
                     /gene="rplL"
                     /locus_tag="PKH07_03300"
     CDS             4050..4430
                     /gene="rplL"
                     /locus_tag="PKH07_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HNT34003.1"
                     /translation="MTKEEIIQAVESMTVLELSELVSALKDKFGVSAAMPMMAGPMMP
                     GMGGGAAEPVEEKTEFDVVLKDAGAQKIKVYKAVREVTDLGLKEAKDLVDGAPATVKE
                     GLSKEDAEKMKAKLEESGATVELK"
BASE COUNT         1163 a         1014 c         1399 g          989 t
ORIGIN      
        1 cgattcgcga aggcggacgg accgtgggtg ccggtgtggt cacggagatt attgaataaa
       61 ctagttcagg cggtggtaaa cagaaatgcg cgaaagaatc acattggcgt gtacggactg
      121 caagcagaga aactacaaca cgacgaaaga caaacggaag cagaccgcgc gcttcgagat
      181 taagaagtac tgtcggtttt gccgaaagca tacggcgcat cgtgagacgc ggtaaaggat
      241 gctttcgggt gatgagagga tctcggaaaa cgatagggca gtagctcaat tggtagagca
      301 cctgactcca aatcaggggg ctgggggttc gagtcccttc tgccctgttc ctcgttccga
      361 gcaggccggc gcctgcaagc gtgtgggcta tgcccactct tacgaacgct tgttcattgg
      421 aagaaacaga cttcttcaaa gtcttgtttt ctgggagcag agaagcgtcc gagagtagag
      481 tgacagagac agtttgtgag ccgttcgttt gagacagaaa ccgagctgct cttgagtcaa
      541 gtttcttggg agagagtgaa tctgcgatga ttgccaaaat gaatgcgaca ctgaaagact
      601 ggtacaccaa gacggttgcc tacatcaagg atgtgcgacg ggaactgaac tttgtgacat
      661 ggccgacccg gcaagagatc aaggattcaa ccaagatcgt cttcgtgacg gtcattgtca
      721 tcgccatcta cattgccgtc ttggatggtg tttttacgaa tgtgttcaag ttcatcttta
      781 cgcgtttctg atctgttgag aatgcaggtg gccgggggcg cggtacccac tgcaacgcga
      841 aggatgagga gaaggtttca ggacagttac agaggctgag cggaaagggc tgaagatttg
      901 aacgaaaatc acgaacaaga acccttggag aaccaaggcc aggctcccga tcaagttggt
      961 gctgatccgt ctgaaacacc ggtcaacccg gcggcgtctt ggtatgcggt tcacacctac
     1021 tccggctatg agaacagagt tcgcgaaagc ctcttgaacc ttgttcgcag tgatttccgt
     1081 cttaaggaca agatcatcga ggttcttatc ccgacggaag aggtcgccga gattcgtgaa
     1141 ggcaagaaga gggtatcgtc caagaagttg tttccgggct atgtggtcgt gcatatggag
     1201 atgagtgatg aattgcagac cttggtgcgt tccacccccg gagtgaccgg ctttgtcgga
     1261 ccccgacgca ctcctgttcc cttgaccacg gacgaagtag atcacattct gggtcgcatg
     1321 aaggagaccg aggaaactcc tcgaccgaag gtggttttcg agtgtggcga gaaggtcaaa
     1381 gtcatcgagg gaccgttcga gagctttacc ggctatgttt cagagatcaa ccaggatcga
     1441 ggccgtgtga ccgttatggt agacattctg gggagatcca ctcctgtgga gttggatttc
     1501 cttcaggtgg ataaactctg agttcgaaac agagaggggt ctgtcggaga atcaggtgtt
     1561 ggtgaggaca taggtcatgg gcccgcgggc acagaggccc gctctggagg aaacacgaga
     1621 tgtattgcag aaactgcgga aaagaagttg atgaacatgc cgttgcctgt cctggatgcg
     1681 gagtcgctcc caagtcgcag aagaaattct gtttcaactg tgggcaggag actaatccgg
     1741 ctcaggtgat ctgcaccaag tgcggggtca gccttgcctc aaccctatcg cttggcccca
     1801 agaacaaact cgtcgctggt ctgcttggca ttctgctcgg ctccttgggc atccacaaat
     1861 tctaccttgg atataacaag gaaggaatca tcatgcttgt ggtctctgtg gcggggggaa
     1921 tggttacctg tggggcgggc tctgtcgttg tgggaattat tgggctgatc gaagggattc
     1981 tgtatctgac gaagtctgac gaggagtttg accgcgtcta tgtgcaagga acgaaaacct
     2041 ggttctgagc atgatccatg cgccgaatca ggaattcgaa tagcaattgg tagaaaatcg
     2101 aggatagaag gcaatgccac cgaaaaagaa agtacaggcg attgtgaaac tgcagtgtcc
     2161 cgccggtgcc gcgaatcccg cgccacccgt tggtcctgct ttgggacaac atggtgtgaa
     2221 catcatggag ttctgcaggc agttcaacga gcggacaaag actgaatccg gcatgatcat
     2281 tccagccgtg atcatggtct acgcggacaa gagtttcact ttcatcacaa agacaccacc
     2341 cgcggcgatt cttttgaaga aagctgcagg gatagccaaa gcctccggcc aacccaataa
     2401 gaataagatt ggcaaagtca gccgaaaaca ggtcgaagag attgccaaga ccaagatgga
     2461 agacctgaat gcctttgata tggaggcggc ggtcaagatg gtcgaaggta cggcacgaag
     2521 catgggtatt gaggtggagg catgattaag agcaaacgat tggtggatgc ccacaagact
     2581 tttgatcggg cgaagttgta cacacttccg gaagcggtca aacttgtcaa gcagagcgcg
     2641 aaggcgaagt ttgatgagac cattgagctt tccgcaaaga tgggcgtgga tcctcgaaag
     2701 gcggatcagc agattcgcgg taccgttcaa ctgccccatg ggacaggcaa gcaattgcgc
     2761 gtagtggttt tcgcggaagg cgctctggct gaagaagcaa agcaggcggg tgccgactat
     2821 gttggttccg atgatctgac gcagaaaatc gcggggggct gggtggattt tgacatcgcc
     2881 ctggcaactc cggacatgat gcgaaatgtt ggaaaactcg gaaaggtgct tggcccgcgt
     2941 ggcctgatgc caaacccgaa ggcgggaact gtttccgcca agatcggcga ggcagtccag
     3001 gaattcaagg cgggtaaaat tgagtatcgc ctcgacaagc aagccggtat tcatgtgccc
     3061 gttggcaagg catcctttgc gcccaacaaa ctggaagaaa acattcgatc cctggtttcg
     3121 gcgttgatgc gcgccaagcc gtctactgcc aaagggcagt atctgaaggg tttggcactg
     3181 tcttcgacga tggggatcgg cgtcaagttg gatctgagcg atttccggga cgcttaaagg
     3241 agaaacagga acgatcctgg agtaaaacat accgatagat cttgtccgcc cttgttggag
     3301 aagtaaggcg cagcttcggc tgcttaatgc aatgcctgcg gagacgagcg gcgcagagga
     3361 agcttgagaa caagccgttc tgtgcgcgtg gtgccgcaga acggctcttg tctttcgtgc
     3421 aacaggtgtg gaacgaggaa cggtgcaggc gggaggtgac aaggattgct gaaaaaacaa
     3481 cagaaggctg aaatcgtaga ctctttggtg gaacaactga aggacgtcga gtgtgttttt
     3541 ctggtggact atcgaggatt gaaggttgaa gaagtctttc aactgcgaac ggcactccgt
     3601 aagaccgaca gccggatgcg cgttctcagg aatacacttc tgacgcgcgt gttcaaacgt
     3661 tcccagaacg agaaggccgg ggagatggtc gaaggtccga ctgctgtgat ttggactaaa
     3721 ggggatatcg tggaagccgc aaaggcgctg gatgcctttg ccaaggaaca cgaaaacctg
     3781 aagatcaagt tcggcattct cgaaaacaag atcatcacgg ataagagcat tgcggctttg
     3841 gcgaaattgc cgcccaagca ggttctgctt ggtcagttgg tgggtgtttt ggaaggccca
     3901 atctccggat tggtctttgc cctggaaggc gttatcagcg agttggtcta taccttgcag
     3961 gcagttgtgg acaagagaga gcagactgcg gcctgatagg tcgtggctgt cgagaaaaga
     4021 tatacatcga aaggaattga ggatacaaaa tgacgaagga agaaatcatt caagcagtag
     4081 agagcatgac cgtgctggaa ttgtctgagc tggtcagcgc attgaaagac aagtttggtg
     4141 tctcggctgc aatgccgatg atggctggtc ccatgatgcc cggaatgggt ggtggcgcgg
     4201 cagaaccggt cgaggaaaag actgagtttg acgtcgttct taaggacgcc ggtgcacaga
     4261 agattaaggt ctacaaggcg gttcgagaag tcaccgacct gggcctgaaa gaagccaagg
     4321 atctggttga tggcgctcct gcaaccgtca aggaaggcct ctccaaggaa gacgccgaga
     4381 agatgaaggc gaagttggaa gagtctggcg ctaccgtcga actgaagtaa gcttgtcgtt
     4441 gtccgcatgc atggtctttt caggctgcgg gcattaagta gttggctgag aacacaacgg
     4501 ctctctctgc tgtgggtgga gagagccgtt actgtcttct cgcctcgtgc cacttgggat
     4561 aacaa
//
LOCUS       DAOQUC010000123         4553 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_21154_length_4553_cov_3.171187, whole genome shotgun sequence.
ACCESSION   DAOQUC010000123 DAOQUC010000000
VERSION     DAOQUC010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4553)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4553)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4553
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21154_length_4553_cov_3.171187"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..549)
                     /locus_tag="PKH07_03305"
     CDS             complement(<1..549)
                     /locus_tag="PKH07_03305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34004.1"
                     /translation="MKRSLWLTQVSACCVCLFCVILAIPCFSMVYVDTNAGPGGDGNS
                     WLTAYQSIQAGISDADLADEDVWVAQGTYPEAISMATGVAVYGGFQSGATLFSERNPK
                     MYVAIIDGATAGDYGYPAEHVVLFYSTTNAVLDGFLVTGGYASYSGGPWAIGGVGGGV
                     YCQEANSSNRITNCTISGNVALS"
     gene            complement(801..1934)
                     /locus_tag="PKH07_03310"
     CDS             complement(801..1934)
                     /locus_tag="PKH07_03310"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNT34005.1"
                     /translation="MTPKQRMMTAMNKGQPDRVPAAPDMSNMIPCRLTGKPFWDIYLY
                     QNPPRWLAYIKTVKYFGTDGWLDSVPLEFPDEVETRQRQPQWREAIVHRTEDRIYTRH
                     HLNDGANHVWSSFCNVYYRADSPTHGVPLAKVGLPEAPPNSWEEVIPKNSYSGLEAFQ
                     TARREMGDLGVVGVPIGLPGLGLAPECVYEYYDHPERVIERCQRQHDATVQRIRRLVT
                     LKPDFVLIGISGFMISNPEPIFRQLALPTLQAITAICKDVGVISQIHCCGPERDLVRI
                     AAEESDLNSINPLEIPPMGDCDLAQIKREYGSRLSLMGNLHTTDVMLRGTPKTVKEAS
                     RKAIQDAADGGGFILSTGDQCGRDTPDENILAMIEAVVEFGNY"
     gene            complement(1969..3327)
                     /locus_tag="PKH07_03315"
     CDS             complement(1969..3327)
                     /locus_tag="PKH07_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020383465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HNT34006.1"
                     /translation="MANSKDISVKEVQIGFEDLMLRSPLKFGGKVLTALTLLNVEVVV
                     KDRLGNRANGIGSMILGNSWSFPSKVHGYDETLGAMKVLAECIQTTLNSCTLAGHPIE
                     LMTDLEPDFLAASENVTRELGLSEVIPKLCTLVVASPFDAALHDAYGRVHNLNVYNAY
                     GSDFMNRDLAFYLTPEFAGEYLEQYTLRTPKPRMPLYHLVGAMDPLTDADVKERIEDG
                     LPNTLAEWIQKDGLTHLKIKLDGNNLDWDVERTLGIEQVSAEVQMQRQIGQWFYSVDF
                     NERCPDVQYLLDYLERIREKSRSCFDRIQYIEQPTSRYLKDHRDVRMHEVAKVKPIVI
                     DEALVDFEHLLLSMELGYSGVALKTCKGQSQALLMAAAALKYNLFLCVQDLTLVGASF
                     LHSAGLCARIPNVAAIEGNGRQYCPNANKVWEQRWDSIFRVTDGTLGTSCLTLPGLGH
                     TE"
     gene            complement(3357..>4553)
                     /locus_tag="PKH07_03320"
     CDS             complement(3357..>4553)
                     /locus_tag="PKH07_03320"
                     /inference="COORDINATES: protein motif:HMM:NF027224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BNR repeat-containing protein"
                     /protein_id="HNT34007.1"
                     /translation="ATKVNTAIFRHHAIATFGKRQFVTFYTPDGCVAIAHRDIPSDSW
                     TIHRTDWKGNVRDAHNCISLGISPDGTLHISYDHHGHPLRYRRSEKPLDPSTFGEIIG
                     MTGQRESRVTYPQFVNLADGTLLFIYRDGGSGNGDLCVNRYDVSSKCWEVLHHPLIAG
                     EGSHNAYWCRPAVGKDGSIKLAWCWRRTGDASTNSRICYAQSPDGGVTWEDSGGKPYD
                     LPITQKTNEVIDPVGESNNLSNQDSSEVDSQNRMHITVRKNDSEGIPQFWHIWHDGSS
                     WRQSQVSQFTERFDMKGGGTLRNPLSRPDLFIDRKDNVYVLYRYTTEGSRPMVAKAFP
                     PDYNEWTHFPLAENNLLQWEPNYDILRWKRDGVLNLFLLPSDQGDHETTTEQPPQMVS
                     VLEWKP"
BASE COUNT         1080 a         1244 c         1144 g         1085 t
ORIGIN      
        1 gctcagagca acattcccgg agatggtgca gttcgtgatt ctgttggaac tattggcttc
       61 ctggcagtat accccaccac caaccccacc gatcgcccat ggaccacccg agtatgaagc
      121 atacccaccc gtgacgagaa atccatccag aacggcattc gtcgtactgt agaataagac
      181 aacatgctca gcaggatagc cgtagtctcc agccgttgca ccgtctataa ttgcaacgta
      241 cattttggga ttgcgttctg aaaacagagt ggctccggac tgaaaaccac cgtataccgc
      301 aacacctgta gccatggaga tcgcctcagg ataggtgcct tgcgccaccc acacatcttc
      361 gtcagcaaga tctgcatcac tgataccagc ctgaatactc tgataggctg tcaaccaact
      421 atttccatca ccgccgggac ctgcattagt gtccacatag accatcgaaa agcaaggaat
      481 cgccagaatg acacaaaaga gacaaacaca acacgcactc acctgggtga gccacaaaga
      541 cctcttcatc aacggagcct ccagaatgaa atagaaaacg gagggattct caatccagaa
      601 gggctaataa gtcaagtttt tctcagaaat ggcgtcagtg tccgatgcaa ctttcatccc
      661 aacagaaacc atgcattccg aatattcgga atactggaaa agagagtgct cacacagatc
      721 cctgcttgcg agggactatg agaacgggaa accgcttgtc tgacatcctc tgatcccggc
      781 gctaccgaaa aaccgatcgg ctagtagttc ccaaactcga caacagcctc tatcatcgca
      841 agtatattct catcgggcgt gtcacgccca cactggtcgc ctgtagagag aatgaagcca
      901 ccgccatcgg ctgcgtcctg aatggctttg cgcgaggcct ctttcacggt cttgggtgtg
      961 cctcggagca tgacatccgt tgtatgaaga ttccccatga ggctcagtct gctgccgtac
     1021 tcgcgcttga tctgtgccag gtcacagtct cccatggggg gtatctcgag aggattgatg
     1081 ctgttgagat cggactcctc ggccgcgata cgcaccagat cacgctccgg accgcagcag
     1141 tgaatctggc ttatcactcc gacatccttg cagattgctg tgatggcctg cagtgtcggc
     1201 aatgccagtt gtcggaatat cggttcaggg ttgctgatca tgaatcccga aatgcctatc
     1261 aggacaaagt cgggtttcaa agtgaccaac cgacgtattc tctgaactgt agcgtcatgc
     1321 tgcctttgac aacgttcgat gactcgttcc ggatgatcat agtactcata gacgcattcg
     1381 ggagccaacc caagtcccgg cagtccaatc ggtacgccaa cgacgccaag atcacccatc
     1441 tcacgacgcg ccgtttgaaa tgcctccaac cccgagtagc tgttcttcgg gatgacctcc
     1501 tcccacgagt tcggaggcgc ttctggcaaa ccaactttgg caaggggcac tccgtgagtt
     1561 ggcgaatccg cccgatagta gacgttgcag aatgatgacc atacgtgatt ggcaccgtca
     1621 ttcagatgat gtcgtgtgta gattctgtcc tcagttctat gaacaatggc ttcgcgccat
     1681 tggggctgtc tttgcctggt ctcgacttcg tccggaaact ccaacggcac gctgtcgagc
     1741 caaccgtccg ttccaaaata cttcacagtt ttgatgtatg ccaaccatcg agggggattc
     1801 tggtaaagat agatgtccca gaatggtttg cctgtcagac gacagggaat catgttggac
     1861 atgtctggag cggccggaac gcgatccggc tgccctttgt tcatcgctgt catcattcgc
     1921 tgtttgggtg tcatagggaa gaccctttcg gttggagagc aacccgtgtc attctgtgtg
     1981 accgagtcct ggaagcgtca gacatgaagt ccccaacgtt ccgtctgtaa ctcgaaagat
     2041 cgagtcccag cgttgttccc aaaccttgtt ggcgttgggg cagtactggc gtccgtttcc
     2101 ttcgatggca gctacgttgg gaattctggc gcaaagtccg gcggagtgca ggaaagacgc
     2161 tcccacaagg gtcagatcct gtacgcacaa aaagagattg tacttcaggg ccgccgccgc
     2221 catgagcagc gcctgcgact gtcccttgca tgtcttcaaa gccacgccac tgtatcccaa
     2281 ttccattgag agcaacaggt gttcgaaatc caccagcgct tcgtcaatga cgatgggttt
     2341 gaccttggcg acctcgtgca tcctaacgtc tctatgatcc ttgagatacc ggctcgtcgg
     2401 ctgctcaatg tactgaatgc ggtcgaaaca cgatctgctt ttctctcgga tgcgttccaa
     2461 ataatcgaga aggtactgca catctggaca acgctcattg aagtccacac tgtagaacca
     2521 ttgaccaatt tggcgctgca tctgaacttc tgcggacact tgttcaatgc caagtgtccg
     2581 ctcaacatcc caatcaaggt tgttcccatc gagtttgatc ttgagatggg taagtccgtc
     2641 cttctgaatc cattctgcaa gtgtgttggg aagtccgtcc tcaatgcgct ctttcacatc
     2701 cgcatctgtc agaggatcca tcgctccaac caaatgataa agaggcatcc gaggcttggg
     2761 tgtcctgagt gtatactgtt cgaggtactc acccgcaaac tcgggtgtga gatagaatgc
     2821 gagatcgcga ttcatgaaat ccgaaccgta ggcgttgtat acatttagat tgtgaaccct
     2881 gccgtatgcg tcatggagag cagcgtcaaa gggtgaagca accacaagcg tacacagttt
     2941 cggaatgact tcactcagtc ccagttcccg ggtcacgttc tcagaggcag ccaggaaatc
     3001 aggctccaga tcggtcatca gctcgatcgg atgtcctgcc agggtgcagg aattcagtgt
     3061 ggtctgaatg cactctgcaa gaaccttcat ggcgccaagt gtctcgtcat atccatgcac
     3121 tttgctgggg aatgaccatg agttgccaag tatcatggag ccaatcccgt tggctctgtt
     3181 gcccaatctg tctttgacaa cgacttccac gttcagcaga gtgagcgccg taagcacctt
     3241 ccccccgaac ttcaatggcg aacgaagcat cagatcttcg aagccgatct gtacttcttt
     3301 gactgatatg tctttggagt tggccatgca aaccaccact tcagttggca cccggactat
     3361 ggcttccact ccagaacaga caccatctgc ggcggctgct ctgtggtggt ttcatgatcg
     3421 ccttgatccg aaggaagcaa gaacagattg agaacgccat ccctcttcca cctgagaatg
     3481 tcgtagttgg gctcccattg cagcaagttg ttctctgcca agggaaagtg agtccactcg
     3541 ttgtaatcag gcggaaaggc cttggcgacc atcggccgag agccttcggt tgtgtaccgg
     3601 tacagcacgt agacattgtc cttacgatca atgaaaagat cggggcggct gagtgggttg
     3661 cgcagggtgc ctcccccctt catatcaaaa cgctccgtga attgagagac ttgactttgt
     3721 ctccaggaag aaccgtcatg ccagatgtgc caaaactgcg gaatgccttc agaatcgttc
     3781 tttctgactg taatgtgcat ccgattctga ctgtccactt ctgaggaatc ctggttgctg
     3841 aggttattgc tctcgccaac aggatcaatg acctcattgg ttttctgagt gatgggtaga
     3901 tcgtaaggtt tcccgcctga gtcttcccat gtgacaccac cgtcaggact ttgggcgtag
     3961 cagattcggc tgtttgtcga agcatcgcca gttcgtcgcc agcaccacgc gagtttgatg
     4021 cttccgtcct ttcccacggc agggcgacac caataggcgt tgtgagaacc ttcaccggca
     4081 atcagcgggt ggtggagcac ctcccagcac ttacttgaca cgtcgtagcg attgacacac
     4141 aagtctccgt tgcccgagcc tccatcgcga tagatgaaaa gaagagttcc atcagccaga
     4201 ttcacaaact gtggatacgt cactcggctc tctcgctgac ccgtcatgcc gatgatctct
     4261 ccgaaagtgg atgggtcaag cggcttttcg ctacgtctat atcgaagagg atgcccatga
     4321 tgatcgtaac tgatatgcag agtgccatct ggagaaatgc ccaaactgat acagttgtgt
     4381 gcatcccgaa catttccctt ccaatcagtg cggtggatcg tccaggaatc cgaaggtata
     4441 tcacgatgcg ctatagccac acatccgtcg ggagtgtaga acgtgacgaa ctgccgcttt
     4501 ccgaacgtcg caatggcatg gtgtcgaaag attgccgtgt tgaccttggt tgc
//
LOCUS       DAOQUC010000124         4552 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_21159_length_4552_cov_2.684679, whole genome shotgun sequence.
ACCESSION   DAOQUC010000124 DAOQUC010000000
VERSION     DAOQUC010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4552
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21159_length_4552_cov_2.684679"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..347
                     /locus_tag="PKH07_03325"
     CDS             <1..347
                     /locus_tag="PKH07_03325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34008.1"
                     /translation="IMSTHPPTSERIDNLKAQSVAEGKIRLPVLQFRAWLSPFDLGVS
                     HDAEIGLELREDRSIHQFYLKARLFSGDQQNWHRLTPTFIQILRKQFLMWRILSDAAK
                     EQYIGKGESLFR"
     gene            360..2447
                     /locus_tag="PKH07_03330"
     CDS             360..2447
                     /locus_tag="PKH07_03330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide transporter"
                     /protein_id="HNT34009.1"
                     /translation="MTDLENMAQESKPTESISLGPTQDDVLERGPFVDGFSRKTIIGA
                     LFVAFVMMPGSIYLGLVAGQTLGSAAEWVTIILFAEVARRSFTNLKRQEVFILFYVAS
                     AIAAVTMAHLALSGGPFAGAIWNQYLVQSPQTSAISDQIPDWVVPAASSQAIRGRNLA
                     HPDWFFSKSRGLLGPFVLCAIGYVLGRFAWFGMGYLVFRATSDLERLPFPLAPIAAEG
                     ATALAESTDREENVFRSKRSWRWNIFSIGASMGVVFGCLYVLLPVVTGLFLAKPIMLF
                     PIPFIDFTKNVESVLPASLISLSFDIGGLLAGMILPFPLVIGLFISIMTTGVFGNPIL
                     LKLGAFRHWTPGNGLLVNQMILSFDFWMSVSIGMAAAVAVLGISNMIRTFARQIRTRG
                     HNGRRREIELDSFGVPLRAPSKQRGDFPLWIAAGLFVFATAGFAAIAHILVPEFPVWI
                     ILIFGFIWSPLHSYISARMVGLTGGGLATPFLKETVFITSGYKGVDIWFAPIPLFDCG
                     GMAQRFRELELTRTKFTSIFKAELLMFPIIFISSFVFWWFFWKLNQIPSDTFPFAARM
                     WPVAARQAYLIFTANSTGNALLLQALNPKVIVGSLGVGMALYTVVQLSGLPVIFFYGL
                     IGGLGGALHGALPLITGALLGRYYFGKKFGADRWKRYVPVLAAGFSCGMGLAGMTAVA
                     FSLIAQCARDLPF"
     gene            2472..3647
                     /locus_tag="PKH07_03335"
     CDS             2472..3647
                     /locus_tag="PKH07_03335"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNT34010.1"
                     /translation="MTGREKILAALSDDGTPEIPVVICYEGILIRDHWNQLTACPWWY
                     QESPDLDHQIAWRRDFFDKVPQDWFYMPLCVSRAQRKSLSIRESDGVVALVDRQTGHE
                     RVLEPPVVGGKMIPRDTASLTGNSIDVSTEDIDRGVPIKILDVQAMRKEGRFDLSDRI
                     LAGLGADRFPMTSVHSPLWDASGLWGFEALMTLVATQPELVQRACERFLQRSIQQVQC
                     ASARGVLGIWIEECFTDMIGPKSYRTLCLPYLRRLIEAIRDIGLYSVFYFCGDPKNLW
                     ELLLDAKADALSLEEGKKGFEIDIEDVVSRVQGRCTLLGNLDAFHVLEQGTDSELHDE
                     IRRQIAAGRANGNRFITSLGSPVTPNTSMESLARYLTLARESAKTPLRSRKKGAMQA"
     gene            3806..4378
                     /locus_tag="PKH07_03340"
     CDS             3806..4378
                     /locus_tag="PKH07_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HNT34011.1"
                     /translation="MGAMIEIRWHGRGGQGAKTAATSLAEAALLEGKYSQGYPDYGPE
                     RMGAPMRGFTRISENPIRVHCEINHPSAVAVLDPTLLGVVNVADGLQADGAIVVNTKD
                     TPAAIRKRLGVSNVKVFTVDANSISMETIGRTMPNTPMLGALLKVTGALKLDTMLEDI
                     KKKFGKKFSQKVVEGNLNAIRRAYEEVQGE"
     gene            4375..>4552
                     /locus_tag="PKH07_03345"
     CDS             4375..>4552
                     /locus_tag="PKH07_03345"
                     /inference="COORDINATES: protein motif:HMM:NF012267.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNT34012.1"
                     /translation="MKVKPNSGWKDIPIGGLIDEPGTAAEYVTGTWRTYKPVWSSEKC
                     IQCLRCWIFCPDSAV"
BASE COUNT         1007 a         1186 c         1229 g         1130 t
ORIGIN      
        1 aaatcatgag cacgcacccg cccacgagcg aacgcattga caatctgaaa gcacaaagcg
       61 tagcggaagg caaaatcagg cttccagttc ttcagttccg tgcatggctc tccccgttcg
      121 atctcggtgt gagtcatgac gctgaaatcg gcctcgaact ccgtgaagac cgttcaattc
      181 atcaattcta cttaaaagcg cgactgttca gtggcgatca acagaattgg catcgcctga
      241 ccccaacttt tattcagatt ctgagaaagc agttcttgat gtggcgcatt ctttccgatg
      301 ccgccaaaga acaatacatc ggaaagggag agtccctctt ccgatagacc ggacaaaaca
      361 tgactgactt ggaaaacatg gctcaggaga gcaaaccgac tgagagcatc tccctcggtc
      421 ccacgcaaga cgatgttctc gaacgtggtc cctttgtgga cggtttctct cggaaaacga
      481 tcatcggcgc cctttttgtg gcctttgtca tgatgcccgg ttcgatctat ctgggtttgg
      541 tggcaggcca aacgctgggc tctgcggctg agtgggtgac catcatcctg ttcgccgaag
      601 tggcgcgcag aagtttcacc aacctcaaga gacaggaagt tttcatactg ttttatgtag
      661 cctctgcaat cgccgcggtt accatggctc acttggcgct ctcgggtggc ccctttgctg
      721 gagcgatttg gaatcagtat cttgttcagt cacctcaaac cagcgccatc tcagatcaga
      781 ttccagactg ggttgtgccc gcggcctctt cgcaggcaat tcgtggcaga aatcttgcgc
      841 atcccgactg gttcttcagt aagagcaggg ggctgctcgg gccatttgtg ctgtgcgcaa
      901 tcggttatgt tctcggacga ttcgcctggt ttggcatggg ctaccttgta tttcgagcca
      961 catccgatct agaacggctc ccgttcccat tggctccgat cgcagctgaa ggagcgaccg
     1021 ctctcgcgga atccaccgac cgcgaggaaa acgtctttcg aagcaaacgc tcctggcggt
     1081 ggaatatctt cagcattggt gcctcgatgg gcgtcgtctt cggctgtctc tatgtccttc
     1141 ttcccgttgt tacaggactg tttcttgcaa aacccattat gctgttccct attcccttta
     1201 ttgatttcac caagaatgtg gaaagcgttc ttccggcgag tctaatctca ctcagttttg
     1261 atattggtgg ccttctggcg gggatgattc ttcccttccc acttgtgatc ggacttttta
     1321 tctcaatcat gacaaccggc gttttcggga atccaattct gctcaaactc ggtgcttttc
     1381 ggcactggac accaggaaat ggtcttctcg tcaaccagat gatcttgagt tttgatttct
     1441 ggatgagcgt ctcgattggc atggccgccg cagttgcagt gcttggcata tcaaatatga
     1501 tccgaacctt tgccaggcag attcgtacaa gaggccacaa cggaagaaga cgcgagatcg
     1561 agctggactc tttcggcgtc cctctccgag caccgagcaa gcaacgaggt gatttccctc
     1621 tctggatcgc agcggggctc tttgtctttg cgaccgcagg ctttgccgcc attgcccata
     1681 ttctcgttcc tgagtttccg gtctggatta tcttgatctt cgggtttatc tggtcaccac
     1741 tgcactcgta tatctccgca cgaatggtcg gtcttaccgg tggcggactc gccaccccat
     1801 ttctcaagga aactgtgttc atcacaagtg gttacaaggg cgtggatatc tggtttgcgc
     1861 caatccctct ttttgactgc ggtggaatgg cacagcgttt ccgagagctg gagttgaccc
     1921 gaaccaagtt cacttcgata ttcaaggcag aacttctgat gtttccgatc atctttatca
     1981 gcagttttgt gttttggtgg ttcttctgga aactcaatca gattccttcg gacacctttc
     2041 cctttgctgc ccgaatgtgg cccgtcgcag cacgacaggc ttaccttatc ttcacggcca
     2101 acagcacagg gaacgccctg ctgcttcagg ccctcaatcc caaagtcatc gttggttcct
     2161 tgggagtcgg gatggccttg tacacggtgg ttcagctatc agggcttccc gtcatcttct
     2221 tctacggtct catcggtgga ctcggtggtg ccctgcacgg cgctctgcca ctgatcactg
     2281 gagcattgct cggaaggtat tacttcggca agaagtttgg cgcagaccgg tggaaacgct
     2341 atgtccccgt tttggctgcc ggtttctcct gtggaatggg attggcagga atgaccgctg
     2401 tagcattctc cctcatcgct cagtgtgcgc gtgatctgcc attctgatcg aattggggtg
     2461 gtgaaaagac catgacagga cgcgagaaaa tccttgcggc actttccgat gacggtaccc
     2521 cggagatccc tgttgttatc tgctacgaag gaattctcat ccgagatcat tggaaccaac
     2581 tgaccgcatg tccgtggtgg tatcaggaaa gtcctgatct cgatcatcaa atcgcatggc
     2641 gccgtgactt tttcgacaag gttccccagg attggtttta catgccactg tgcgtgtcac
     2701 gtgcgcagag aaagtctctc tcaattcgcg agtctgatgg tgtggttgct ctcgtggatc
     2761 gtcaaaccgg acacgagcgg gtattggagc cccccgttgt tggaggcaag atgatcccac
     2821 gcgatacggc ctccctcaca ggcaacagca ttgacgtatc aacagaagac attgatcgcg
     2881 gtgttcctat taagattctt gatgtgcaag ccatgcgcaa ggaaggccga tttgatctct
     2941 cagatcggat tctcgccgga ctgggtgccg atcggtttcc gatgacgtct gtgcattcac
     3001 cgctctggga tgcgtcaggg ctctggggat tcgaagcctt gatgactctg gtggcaacgc
     3061 agcctgaact ggtgcaacgt gcctgtgagc gattcctcca acgctcaata caacaggttc
     3121 agtgtgcctc agcccgtggt gtccttggaa tctggattga ggagtgtttt acagacatga
     3181 taggcccgaa atcgtatcgg actctctgtc ttccctattt gcgacgtctc attgaagcca
     3241 ttcgcgatat cggactatac agtgtcttct atttctgcgg agatcccaag aacctctggg
     3301 aattgctgct ggatgccaaa gcggacgccc tctccttgga agaggggaag aagggattcg
     3361 agatagatat cgaggatgtg gtcagccgag tacagggcag atgtaccctc ttgggaaatc
     3421 tggacgcatt tcatgttctc gaacagggga ctgactctga acttcatgac gagatccgaa
     3481 ggcagatcgc cgcgggacga gccaatggca atcgcttcat caccagcctc ggaagtcctg
     3541 taactccaaa tacatccatg gaaagcctgg ctcgttatct gacactggca agagagtctg
     3601 ccaaaacgcc cctcagatcg cgaaaaaaag gcgccatgca ggcttgacta ttctcaggag
     3661 caagtgctat atatatccgt ttgtttgagt ggcgtggaac tggaacgcag atgagtctga
     3721 aagaaggtct agaggtgtga aaaagtgggg cggaccccat aaagatccgc cctgtaggtt
     3781 ttgagaaagg agagagtgaa cgagaatggg agccatgata gaaatcagat ggcatggacg
     3841 tggcggtcaa ggagccaaga cagccgcgac gtctctggct gaagctgcgc tcttggaagg
     3901 caagtacagc caaggctatc cagactacgg tccggagcgc atgggtgcgc cgatgcgggg
     3961 cttcacccgc atcagcgaga acccaatcag ggtgcattgc gaaatcaacc accccagcgc
     4021 agtggccgtt ctggatccga ctttgcttgg cgtggtgaat gttgcggatg ggcttcaggc
     4081 ggacggagca atcgtcgtca acacgaagga cacgccggct gcgattagga agcgactggg
     4141 tgtctctaac gtgaaggtct tcactgtgga tgccaactcc atctccatgg agacgattgg
     4201 gcgcaccatg cccaacaccc caatgctggg cgcacttttg aaggtcactg gagcgctcaa
     4261 actggacaca atgctggaag acattaagaa gaaattcggc aagaagttct cacagaaggt
     4321 tgtcgaagga aatctgaatg ccatccgcag agcttatgag gaggttcagg gcgaatgaag
     4381 gtcaaaccga acagtggttg gaaagatatc cccatcgggg gtttgattga cgagccgggc
     4441 acagccgcag aatacgtcac aggtacttgg cgcacctaca aacccgtctg gagttcagaa
     4501 aagtgcattc agtgccttcg ctgttggata ttctgcccgg attccgctgt ca
//
LOCUS       DAOQUC010000125         4533 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_21289_length_4533_cov_2.954891, whole genome shotgun sequence.
ACCESSION   DAOQUC010000125 DAOQUC010000000
VERSION     DAOQUC010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4533
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21289_length_4533_cov_2.954891"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..697)
                     /locus_tag="PKH07_03350"
     CDS             complement(<1..697)
                     /locus_tag="PKH07_03350"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNT34013.1"
                     /translation="MAKAKRLSAVLFLIVIGIGSVWAETPVTEIDPFVVVKANDALSL
                     RQWLAQDGDPNLRVKGTATLLHIAVLSRSSDCVRVLLEHGAVVDATPDFYGAPLRWVQ
                     DVTSASYLIAAGADVNAIDREGDSLLDLWVKLGKPQSELVRLLIESGANIEHRNARGE
                     TPIHAAASFGSWEAFSLLLDKGADAHSVDCNGRTSLHSAAEGGNIKIAKKLINSGLDV
                     NATDEGGYTPLHIA"
     gene            965..2074
                     /locus_tag="PKH07_03355"
     CDS             965..2074
                     /locus_tag="PKH07_03355"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNT34014.1"
                     /translation="MSIWKRTTSLSGQCSTEPFEMWESPDKSRKTFTVHATYGKPIDI
                     LSLKLDVLEDFRAQVEHVKKTRSALFSSPEIEIVQACPICNAPAEQSRFSVQIYNARY
                     HECLSCTHRFVINRPGQRALDRFYSEDAPIASTYTDRRTSETRVKQVATPKAQWALAE
                     FERVHGTKPERILDVGAGGGHFVYACHLLGLNADGIELSESSRDYCKRHFEIDLRAED
                     FLSNDLSLPEYDLITFWGVIEHVAEPVQFLNAARKHLSQHKGMVIAEVPRWECLGTAI
                     QSQWNDSLVRHLNPMGHIHIFSDNSLATAFANASLAPAAVWYFGMDAYEWATQMSYHL
                     DDPSVVEKIKELIPGVQQSLDSARFSDEVVLAAIP"
     assembly_gap    2195..2204
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2205..4130
                     /locus_tag="PKH07_03360"
     CDS             <2205..4130
                     /locus_tag="PKH07_03360"
                     /inference="COORDINATES: protein motif:HMM:NF014075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF115 domain-containing protein"
                     /protein_id="HNT34015.1"
                     /translation="PNMDVLQKNLAALDQTNPALAAELLHLEQKDVSPAGFTLSAVET
                     PSGLFSLAADNEQGSRILLHSARDPKREASRMIQSLAPEPGDSFVVFGIGMGHHLLEL
                     FRAVESQMGFCLVIERSPQILRKALEHSDLSHLLSDARLSIVCGDALTEFSVALRERI
                     LKALLGGVRLIDHRPSISLYPEFYQDATQRVIDMVLQGRSDVQTALLKGTEFQRNVIL
                     NLPDSVHATGVANFEGKFTGCPAVVVAAGPSLDKNIELLRQVQDSALIISVNTSFQTL
                     RERGITPHIVTTIDPSETTLKHFNRTDVVNDSILAFDSECYSKVQSKFAGQKMFVGIK
                     GLRIVQWLEQTLGQIGFLEKGNTVAHTSFYLARMMGCDPIILVGLDLALPPEEGPTHV
                     AGASLRGRYRKDPNDRNHVIISDPHDPSKLQRRKVFWVPGITAPEVPSLDILYVYLRQ
                     FEREFHQTKQTVIDATEGGASKQGTQIMTLADAIAQYCQSRQPIREKLEGCLKPSPST
                     EQIQMIVQGLRTAQAQLDTARESAQRGYRLSVSLEEFFQGERSSAKQAERQMKEMNTL
                     LDQMGTYHLALAFLQRYIAGVHFLFSQRLKRASTPEQQKERAIEFVKRRKLLFDAIRI
                     CSSEAISLIEEIIPRFS"
     gene            complement(4146..>4533)
                     /locus_tag="PKH07_03365"
     CDS             complement(4146..>4533)
                     /locus_tag="PKH07_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020682885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gamma-glutamyl-phosphate reductase"
                     /protein_id="HNT34016.1"
                     /translation="GCEETRIICPQVKSATEEDWSTEYLDLILSIRVVRDFDEAVAHI
                     EKYGSHHTDTIVTRDWETAHRFVQQVDSSSVMVNVSTRFSDGFQYGLGAEIGISTDKL
                     HARGPMGLEELTTQKFIVFGNGQERT"
BASE COUNT         1059 a         1195 c         1202 g         1067 t
ORIGIN      
        1 ccgcgatatg caatggcgta tagccgcctt catctgtggc attcacgtcc aacccgctat
       61 tgataagttt cttggcaatc ttgatgttgc caccctctgc cgccgaatgc aggctcgttc
      121 ttccattgca gtcaacagag tgagcgtcgg cgcccttgtc cagtaagaga ctgaaggcct
      181 cccaagagcc gaatgaagcc gctgcatgaa tcggtgtctc accgcgggcg ttccggtgtt
      241 caatatttgc cccgctctct atcaaaagac ggaccaattc agactggggc ttgcccaact
      301 taacccagag atcgaggaga ctatcgcctt ctcggtcaat ggcgttgaca tctgcccctg
      361 cggcaatgag ataactggca cttgtgacat cctgaaccca tctaaggggt gctccataga
      421 aatcaggtgt ggcatctaca actgcaccat gttccagaag tacccgtacg cagtcagagg
      481 aacgcgacaa gacagcgata tgcagaagtg tggcggttcc cttgactcgc agatttggat
      541 ccccatcttg tgccaaccat tggcgtagcg agagcgcatc attagctttt acgactacaa
      601 agggatcaat ctcggtaacc ggagtctccg cccagaccga gccaatgccg atcacaatga
      661 gaaacagaac ggcagacagt ctttttgctt ttgccatcgc ttcaaggcct cctgcatatc
      721 cgtatagagc tgcagagcac aattctgtcc ggaacttcct aaatgtagcc tatagaagcc
      781 accttttcaa tcccaagtct tggcaacaaa tgagggatat cccaagaccg gatgttgtgt
      841 catttgtaca ccagatcttc ttgaaagcag caaagtaaac cgctgacgtg gtttgactat
      901 gttactgata aacatatacg atacgtcatc agatataatg tgtttgtcaa cggagtgaat
      961 catcatgtcc atttggaaga gaacgacgtc cctttcgggc caatgttcga cagagccctt
     1021 cgaaatgtgg gagtcccctg ataaatcgcg caagaccttc accgtgcatg caacctacgg
     1081 taagccgatt gacattttgt cgctgaaact cgatgtgctg gaggattttc gtgcgcaggt
     1141 ggagcatgtc aagaagactc gcagcgccct cttctcttcc ccggagattg aaatcgttca
     1201 ggcgtgtcca atctgcaatg caccggcaga gcaaagccgg ttcagtgtcc agatctataa
     1261 tgcgcgctat catgaatgtc tgtcttgcac acatcggttt gtcatcaacc gacctgggca
     1321 gagggcctta gaccgcttct actctgagga tgctcctatc gcatccacct acaccgacag
     1381 acgcactagt gagactcgcg tgaagcaggt cgccacaccc aaggcacagt gggctctggc
     1441 ggagttcgag cgcgtccacg gcacaaaacc tgaacgcatt ctcgatgtcg gtgccggtgg
     1501 cggacacttt gtctatgcct gtcaccttct cggactgaac gcggacggga ttgaactgag
     1561 cgagtccagc agggattatt gtaagaggca ttttgagatc gatcttcgtg ccgaggattt
     1621 tctgagcaac gatctgtcgc tgccggaata tgatctaatc acattttggg gagtgattga
     1681 gcatgtcgcc gaaccggtgc aattcctcaa tgctgcccgc aagcacttgt cacaacacaa
     1741 gggaatggtc atcgcagaag tgccacgctg ggagtgcctt ggaactgcga tacaaagtca
     1801 gtggaacgat tcattggttc gccatctgaa tccgatgggt cacattcata tcttcagcga
     1861 caactctctc gccacggcct ttgcaaatgc ctcattggct cctgccgcag tctggtactt
     1921 cggtatggat gcctatgaat gggcaacgca gatgtcttac cacctggatg acccatccgt
     1981 tgtggagaag atcaaggagc tcattccggg tgtgcagcaa tccctggaca gtgcccgatt
     2041 ctctgatgag gttgtgctgg cagccattcc atagagcagc aaggggctgc gaccttgact
     2101 cagattggca cagaaggtac gcttgttcat gtgggtttgc actgacaagc ctgtagttgg
     2161 caacggagat gatctttgtc cgttgtgcgg taagnnnnnn nnnnccaaac atggatgtgc
     2221 ttcagaagaa tctcgcagct ttggatcaga ccaatccggc gcttgcggca gaactgttgc
     2281 atcttgagca aaaggatgtt tcaccggcag gatttacgct ctctgcggtg gagactccat
     2341 caggtctttt cagcttggca gccgacaatg agcaaggtag ccgtatcctg ctgcacagcg
     2401 cacgggatcc aaagcgcgaa gcatcgcgga tgatccagtc actggctcct gaacctgggg
     2461 acagtttcgt cgtctttggc attggcatgg ggcaccatct gttggaacta ttcagagccg
     2521 ttgaaagtca gatgggtttc tgccttgtga ttgagcgcag ccctcaaatt ctgcgaaagg
     2581 ctctggagca cagcgatctg tctcatcttc tcagcgacgc ccggctgagt atcgtctgtg
     2641 gtgatgcgct cacagaattc tccgttgctc ttcgggagag aattctaaaa gcgttgttgg
     2701 gtggcgtcag attgatcgat catcgcccgt ccatctcact gtatccggag ttctatcagg
     2761 atgccactca acgagtcatt gatatggtgc tgcaaggacg ttcggatgtt cagactgcac
     2821 tcctgaaggg cactgagttt cagcgtaacg tgattctgaa tctccctgat tctgtgcatg
     2881 caacaggcgt ggcgaatttc gaggggaaat tcacaggatg tcccgcggtg gtcgttgcgg
     2941 caggcccatc cttggacaag aacatcgaac tactgaggca agtgcaggac tccgcgttga
     3001 tcattagcgt gaacacctcc ttccagactt tgcgggagcg aggcatcact cctcacatcg
     3061 tcacgacgat cgatccctcg gagacgacac tgaagcactt caaccgaacg gatgttgtga
     3121 acgactcgat tctggcgttc gactccgagt gttactccaa ggttcagtcc aagtttgcgg
     3181 gacagaaaat gtttgtcggg atcaaaggac tgagaattgt tcagtggctg gaacagacgt
     3241 tggggcagat tggttttctg gagaagggaa acacggtggc gcacacgtca ttctacctgg
     3301 cgcgaatgat ggggtgcgat ccgatcattc tggtcggttt ggatttggct cttccccccg
     3361 aagaagggcc aacgcacgtc gccggtgctt ctttgcgcgg cagataccgc aaggatccaa
     3421 acgatcgcaa ccatgtgatc atttcagacc ctcatgatcc aagcaaactt cagaggcgaa
     3481 aggtcttctg ggttccaggc atcactgcac ccgaagtccc cagtctggac attctttatg
     3541 tctacctgcg gcaattcgag cgcgagttcc atcagaccaa acagactgtc atagacgcaa
     3601 cagaaggtgg cgccagcaag cagggcaccc agatcatgac gctcgccgat gccatcgctc
     3661 agtactgcca gagtcgtcaa ccgatcagag agaagttgga agggtgtctc aagcccagcc
     3721 catccacaga gcagatacag atgattgttc aggggcttcg aactgcacaa gcgcagctcg
     3781 atactgcaag ggagtccgct cagcgtgggt atcgtctgtc ggtttcactt gaggagttct
     3841 tccagggcga acgcagcagc gccaagcagg ccgagcgaca gatgaaggaa atgaatacac
     3901 tgctggatca gatgggcacc taccatctgg ctctcgcgtt tcttcaacgc tatatcgccg
     3961 gagtgcattt cctgttctcg cagcgcctca agagagcctc aacgcccgag cagcagaagg
     4021 agcgtgccat cgagttcgtc aaacgtcgca aactgctctt tgacgcaatt cggatttgtt
     4081 cgagcgaggc gatttctttg atcgaggaga ttatccccag gttttcgtga agagcggaga
     4141 agacgctacg tgcgctcctg accgttgccg aagacgatga atttctgtgt ggtcagttcc
     4201 tccaaaccca tggggcctcg agcatggagt ttgtccgtgc tgattccgat ttccgccccc
     4261 aggccgtact gaaacccatc cgaaaaacgg gtggagacat ttaccatgac ggacgatgaa
     4321 tccacctgtt gcacgaaccg gtgtgctgtt tcccagtccc gcgtcacgat ggtgtccgta
     4381 tgatgcgatc cgtatttctc gatgtgggca accgcttcat caaaatctcg aacgacacga
     4441 atggaaagga tgagatcaag gtactccgtg gaccaatcct cttcggtagc tgatttgact
     4501 tgcgggcaga tgatccgtgt ctcttcgcac cca
//
LOCUS       DAOQUC010000126         4527 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_21326_length_4527_cov_2.895572, whole genome shotgun sequence.
ACCESSION   DAOQUC010000126 DAOQUC010000000
VERSION     DAOQUC010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4527
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21326_length_4527_cov_2.895572"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2910
                     /locus_tag="PKH07_03370"
     CDS             <1..2910
                     /locus_tag="PKH07_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020262324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNT34017.1"
                     /translation="VTRPIEEAVSAVPGVEKVTSNSTEGSSNVRVTFSWGADLDEASN
                     DIRDRLDRVIPRLPDDVDRPSLRKFDLASFPILILGVASRLDPIQLRRIIDEQIKYRI
                     ERVPGVAALDIWGGLEREIHVDINPDKIKALGLSLNQILSRIRAANINLPAGSIDRGN
                     HEVTIRTPGEYSNLDELRHTIIAERQGVPIPLSEVAEVEDSWQRVTRIARVNGEPGVR
                     LSVNKQSGTNTVAVAEGVLKEIDRINEDIPQVKLITIVDTSSFIQNSIKNVGRAALYG
                     GILATAILLLFLRNIRSTAIIATAIPVSVVAAFGLMHFAGFTLNLMTLGGLALGIGML
                     LDNSIVVLENIYRLREEGMQRLEAAVTGSEEVTSAIIASTLTTLAVFLPLIFVRGMAG
                     IMFRQLSYVIGFSLLCSLLTALTVVPMLSGRLLRTSSMQSTVGESLGHKIFRLSESLF
                     QSMESAYKNLLHLSLNHRILTSALALVVLGSSLSLYPLIGTELMPQADEGEVRIEAEM
                     EVATRLELTDAKLQEIERIVREEVPEMESLVSSAGGSPWQGSVSHTGSMRISLVPQAA
                     RSRSSEEIAQVLRRRLSNIPGMTIRVRTSSGLFVFRMMSSGSDKVQVEIRGHDLETAD
                     VLAERISKAVSNVPGITDVDISRDTGTPEETIIVDRRKAADMKLTVQQIAEMLQTVLS
                     GSRASYYREAGDEYRILVQVKDALKMDLSEILDLTLSNSEGEPVVLRNVVTVEPRRGP
                     VRIERSDQERIVTVSANMSGRDMGSIIRDIRETLRSIPVPRDFSIVFGGEYEDQQEAF
                     NELLVGFILAIILVYMVMACQYESLRDPFVVMFSVPFAAIGVILMLFLTDTTFNIQSY
                     IGCIMLGGIVVNNAILLVDTMNLLRRRDGMPLRSAIEEAGRRRLRPILMTALTTVLGL
                     LPLALGIGEGGEAQAPMARALIGGLLSSTLITLVFVPVVYSLFEGLRRNGAEHAH"
     gene            3078..3887
                     /gene="proC"
                     /locus_tag="PKH07_03375"
     CDS             3078..3887
                     /gene="proC"
                     /locus_tag="PKH07_03375"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HNT34018.1"
                     /translation="MRIGFIGGGNMASALIGGVINSGLTPPENIIVCDILEQRRTLLQ
                     KEFFVQVSDSAREVVKSAKTVVLAVKPQNLEGVATEIGGLFTSDQILISILAGKPRSA
                     IIAALGQSVRLVRAMPNLPALVGAAITAIAQMPEDIEAQATASSILSTVGKVIVVPED
                     LMDPVTALSGSGPGYVFYLIEAFVQGGVELGFSAEEALRLTVETFSGSCKLLLERSAE
                     PSELRRQVTSPGGTTAAGLSAFDAGGLKGTIAQGLLAANQRAKELSTPDTK"
     gene            4009..>4527
                     /locus_tag="PKH07_03380"
     CDS             4009..>4527
                     /locus_tag="PKH07_03380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34019.1"
                     /translation="MSAIFQMTNGIVRHFAMLAAGYAPLAAIVAIVLGRGLLLGLLSF
                     SSAKMGAEVLLSVIAFLKYFTQVIFLFLCFSFLLSRYGLYTSHNIFVEFIHDVAFAFF
                     RLVKRVFITERLGILFAGALFFIVAVHTVALASLHGSGHAVMLASFKQTLLILVNAIG
                     FFTLIICMHALLS"
BASE COUNT          992 a         1130 c         1208 g         1197 t
ORIGIN      
        1 gtcacccgtc caattgaaga agcagtgagc gcagttccgg gtgttgaaaa ggtaacttca
       61 aactcaacgg agggcagcag caatgtgcgt gtgaccttca gttggggagc tgatcttgac
      121 gaggcatcca atgatatccg cgaccgtctc gatagagtga tcccacgcct tcccgacgat
      181 gtggatcgcc cgagtctgag aaagttcgat cttgccagtt ttccgatcct cattctaggg
      241 gtggccagcc ggcttgaccc aattcagttg cggagaatca tagatgagca aatcaagtac
      301 cgaatcgaac gcgtcccagg agttgctgca ctggacattt ggggaggcct cgaaagggaa
      361 atccacgtag acatcaatcc ggacaagatc aaggcgttgg gtctctcgtt gaatcaaatc
      421 ctttcgcgca tcagggctgc gaacatcaat ctgccagctg gttccattga tcgagggaat
      481 catgaagtga cgattcgcac tcccggggag tacagcaatc ttgatgaact tcgccatacc
      541 attatcgcgg agcgtcaggg agttccgatt ccactcagtg aagtggctga agttgaggat
      601 tcctggcaaa gagtaactcg aattgcgaga gtcaatgggg aacctggtgt gcgtctctcg
      661 gtgaacaagc aatcgggaac caatacagtt gctgttgcag aaggtgtttt gaaagagatt
      721 gacaggatca atgaggacat accccaggtc aagctcatca ccatcgttga cacctcttca
      781 ttcatacaga actccatcaa gaatgtcggt cgcgcggctt tgtatggggg catcctcgca
      841 acggcaattc tgctcctgtt tctcaggaac atacgaagca ccgcgatcat tgcaaccgcg
      901 attccagttt ccgttgttgc cgcttttgga ctgatgcact ttgcaggatt cacgctcaac
      961 cttatgacgc tgggtggcct ggctttgggc attggaatgc tcttggataa ctcaatcgtt
     1021 gtccttgaga acatttaccg acttcgagaa gaaggaatgc agcgcctgga agccgcagtc
     1081 acgggtagcg aagaggtgac ttccgcaatc atcgccagca ccctgaccac tttggcggtt
     1141 ttcttaccgt tgatattcgt tcgagggatg gcgggaatca tgttccgaca gctgtcctat
     1201 gtcatcggct tctcacttct ctgttctctg ttgactgctc tgaccgtggt tccgatgctt
     1261 tcaggacgcc ttctgagaac atcctccatg cagagcactg tcggtgagtc gcttggtcac
     1321 aagattttcc gactcagtga gtctctcttc cagtcaatgg aatctgctta caagaaccta
     1381 cttcatctgt cactgaacca ccgcattctg acatcagcac tcgcattggt ggtattgggc
     1441 tcatcccttt ctttgtaccc gcttatcggc acggagttga tgccccaggc tgacgaaggg
     1501 gaggttcgta tcgaggccga aatggaggtt gccacacgcc tggagttgac cgacgccaag
     1561 cttcaggaga tagagagaat agttcgtgaa gaagtccctg aaatggagag tcttgtctca
     1621 agtgctggtg gctctccctg gcaggggagt gtctctcaca ctgggagtat gcgcatctcg
     1681 ctcgtgcccc aggcagctcg aagtcgttcc agcgaggaga tcgctcaggt actgcgccgc
     1741 agactgtcaa atatcccagg aatgacgatc cgtgttcgga caagctctgg cctgtttgtc
     1801 tttcgaatga tgtcgagcgg atcggacaag gttcaggttg aaatccgtgg ccatgatctg
     1861 gagacagctg atgttcttgc agaacgcatc agtaaggcag tatccaatgt cccgggaatt
     1921 actgacgttg atatcagcag agatacgggg acacccgaag agacaatcat cgtggatcgc
     1981 cgcaaagccg cagacatgaa attgacagtc caacagattg ccgagatgct gcagaccgtt
     2041 ctctcaggaa gtcgagccag ctattaccga gaagcagggg atgagtaccg gattcttgtg
     2101 caggtcaagg atgcattgaa gatggacctc agtgagatcc ttgatctgac tctcagcaat
     2161 tcagagggtg aacctgttgt gcttcgaaac gttgtgacgg tcgaaccccg tcgcggaccc
     2221 gtccgtattg agcgatcgga tcaggagcga atcgttacag tttccgcaaa catgagtggt
     2281 cgtgatatgg gttcgattat tcgagacatt cgagagaccc ttcgttcgat tccagttcca
     2341 cgtgacttca gcattgtgtt cggaggcgaa tatgaggacc aacaggaagc gttcaatgag
     2401 ctgctcgttg gtttcattct ggcgatcatc cttgtctaca tggttatggc atgtcaatac
     2461 gagtcactcc gtgatccctt tgtcgtcatg ttctctgttc cattcgcggc gatcggtgtc
     2521 atcctgatgc tcttcctgac cgacaccact ttcaacattc agtcctacat cggctgtatc
     2581 atgctcggag gcattgttgt caacaacgcc atccttctcg ttgacacgat gaaccttctc
     2641 aggagacgcg atggtatgcc ccttcgttcc gccattgagg aagcgggacg acgacgactg
     2701 agacctattc ttatgacagc gctgacaacg gttcttggtc ttcttcccct cgcccttgga
     2761 atcggtgagg gtggtgaagc ccaagctccc atggcaagag ctctgatcgg agggttgttg
     2821 agttcaacct tgattactct tgtctttgtg cctgttgtct actcgttatt cgagggcctt
     2881 cgtcgaaacg gagcggaaca cgctcattga ttggttcggt ggcaaatcga tcctctgctg
     2941 aggggacata ttctcttggc atcttcaact gaactatctc gatgcaagac tcagcctttg
     3001 ccgcaatctt gacaccttcc aactgccact ctatgctttg gacggttaga taagcgacat
     3061 tgtccaagga agcaacgatg cgtatcggtt ttatcggtgg tgggaatatg gcaagcgctc
     3121 tcattggcgg agtcatcaac tctgggttga ctccacccga gaacatcatt gtctgtgata
     3181 tattggaaca acgaagaacc ttgctccaga aggagttttt cgttcaagtc tctgatagtg
     3241 ctcgggaagt tgttaaaagc gctaagacgg tagttctggc tgtaaagccg cagaatctgg
     3301 agggagtggc caccgagatt ggtggtctct ttacctcaga ccagattctg atttcaatcc
     3361 ttgcgggcaa gccacgctcg gcaatcatag cagccttagg acaatcagtt cgcctggttc
     3421 gcgccatgcc caatcttccg gcgcttgtcg gtgcagctat cacggcgatt gctcagatgc
     3481 ccgaggacat tgaagcccaa gcaacggcct cttcgatact tagtacagtt ggtaaggtca
     3541 tcgtagttcc ggaagatctc atggatcctg tgacggcgtt gtcaggaagc ggcccgggat
     3601 atgttttcta cctgatcgag gcttttgttc aaggaggggt tgaactcgga ttcagtgccg
     3661 aggaggcact tcggttgact gttgaaacgt tttcgggctc gtgcaaattg ctgttggagc
     3721 gctctgctga accaagtgaa cttcgtcgtc aggtgacatc acccggtggc actaccgctg
     3781 cagggctttc tgcgttcgat gcgggaggac tcaagggcac tatcgcacag ggcttgctgg
     3841 ccgcgaatca gcgtgctaaa gaactgtcaa cgccagatac gaagtagttt ggaggataca
     3901 gtgtctccta tcggtcaaat tgtctatttt gtgacaaatg tccttttttt gatgttgctt
     3961 ttccgctttg tagctgtgca gaagcggtac tgggtacgcg gtggactcat gtctgccatc
     4021 tttcagatga caaacggaat tgttcggcac ttcgccatgc ttgcggctgg ctatgcccca
     4081 ttggctgcta tcgtggccat cgttctgggt agagggctct tgctggggct tctctctttc
     4141 tcatccgcaa agatgggagc ggaggtcttg ctgagtgtca tcgcattcct gaagtatttt
     4201 acccaagtca ttttcctgtt cctctgcttc agttttctgc tctccaggta tggtctctat
     4261 acaagccaca atatctttgt ggagttcatc catgacgtgg ctttcgcctt tttcaggctc
     4321 gtgaagagag tcttcatcac ggaaaggttg ggcatcttgt tcgcgggagc cttgttcttc
     4381 atcgttgctg ttcataccgt agcccttgca tctctccacg gctcaggaca cgcggtgatg
     4441 ctggcgtcat tcaagcagac tttgttgatc ctcgtcaatg caataggatt cttcactctg
     4501 attatctgta tgcacgcctt gctctca
//
LOCUS       DAOQUC010000127         4516 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_21388_length_4516_cov_3.041246, whole genome shotgun sequence.
ACCESSION   DAOQUC010000127 DAOQUC010000000
VERSION     DAOQUC010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4516
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21388_length_4516_cov_3.041246"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..575)
                     /locus_tag="PKH07_03385"
     CDS             complement(<1..575)
                     /locus_tag="PKH07_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020504540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase family
                     protein"
                     /protein_id="HNT34020.1"
                     /translation="MTKKTIVPDPQTCRHILSLSGGKDSTALALYMRDKVPQMEYVFC
                     DTGHELPETKDYLLKIQAHLGREISILNPDRPFEHYLGLYRGVLPDPRTRWCTRMLKI
                     RPFEKYVGDDPVILYVGIRADEMHRKGYISTKSNITAHFPFVEDGIRMNDVVQILEGS
                     GLGLPDYYRWRSRSGCYFCFFQQRREWVGLL"
     gene            complement(572..3439)
                     /locus_tag="PKH07_03390"
     CDS             complement(572..3439)
                     /locus_tag="PKH07_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018466163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34021.1"
                     /translation="MTNTLVSLSAKATNAGYRGVLLVVDELGKLFEYAARYPQKGDVF
                     VLQEIAEQASRSGRCPILLVGILHQSFEDYAQHLDLATRREWAKIQGRFEDIAFLEPP
                     EQVIRMIAKAIEWSPDATAQIPARFLDKIVLGALKAGIAPPGMKDSDFETASRGAYPI
                     HPVTLVALPFLFRRFAQNERSLFSYLSSLEPYGFQEFIRTKPLEKDCPPVVRLSHLFD
                     YFTKNFGAGLYRHPQALRWMEAADVLERREGLSSLHREVVKTIGVLNALGEFCHLNAT
                     EEVISLAVTDCSVPSSDLIDVLQFLREQSVLTFRKFNRTYRIWEGSDVDIDERIAEGE
                     RQIRHGLNLADRLRQYLPKRPLVARRHSFETGALRYFSVEYLDDPDAIEQCLSVPKDG
                     GTVVVCLGESDMLTERFREIALVSKACRNTLFAIPQFIGELRAIVTEIGALRWAWENT
                     PEIRDDRVARKELSLRIASVEQILWRNVDSLMDPRDEPIGSGCLWIHDGRCIPVSSPA
                     GVSQLLSDVCDQVYPSAPRIRNELISRRLISSAAAAARRNLIERMLTRHSEEALGIEG
                     YPPERSAYESVLRVSGLHRFDEGAWRFCDPDSSDPSNLSALWSFLSDAIFGSKSGPVP
                     LDRLFAQIAEPPFGVLPGLHPVLLCAFLLAHSDETSLYREGTFLPEITVTAFEVLMRR
                     PELFAVGGSRIAGSRALVVNRLARGLGVASATVPIVRALFAMVKSLPEYAWNTQRLSE
                     SVVALREAFRNAKSPEVFLYCMLPEALGMPAFPEGSHTPQEVDRFFDLLNQSLREWAD
                     ATPRTIAGARDSLLNACGLSSGDSGWQQLRELAVAVEPYVTNHQTLSFVQRIAQSAAD
                     AAGVESVIALVASKPPRNWTDADAERFPDAAAAAGASFREAVCTRRISDDPNIELTEE
                     QQGEAEALVVSVKQHLQKTTQKTSPAVIRAAMLKLLEELQT"
     gene            complement(3970..>4516)
                     /locus_tag="PKH07_03395"
     CDS             complement(3970..>4516)
                     /locus_tag="PKH07_03395"
                     /inference="COORDINATES: protein motif:HMM:NF024580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4007 family protein"
                     /protein_id="HNT34022.1"
                     /translation="FNRPDFTRREITDFVRSFAEREGMKVGDSSIMRDVDCLIRTYVA
                     SSGSKKQAVVEETYSCPLLQLNLIQFAPDGDLYRFAIGPKPSLPAAVFAFALHEHFEK
                     TRAGRNTMSLQECLYGEGSPGQVFKLDENSLIEYLEELEDSLEDSIALDETAGLKQIY
                     RRRSIDPLRVLGDYYLGGSRK"
BASE COUNT         1159 a         1302 c         1074 g          981 t
ORIGIN      
        1 tccaatagtc caacccattc ccgtctttgt tggaagaagc agaaatagca ccctgacctt
       61 gatcgccatc tgtagtagtc agggagaccc aaaccgcttc cttcaagaat ctgcacaacg
      121 tcattcattc ggattccatc ttcaacaaac gggaaatgtg cggtaatgtt tgacttagtg
      181 gagatgtagc cctttctgtg catctcatcc gccctaattc ccacatacag gattacaggg
      241 tcatcaccaa cgtatttctc aaagggacga atcttcagca tcctcgtaca ccagcgtgtc
      301 ctagggtcag ggagtactcc acggtacaaa ccgagatagt gttcgaaagg ccgatcagga
      361 ttcaggatac tgatctctct acccaggtgc gcctgaatct tcagcaaata gtccttggtc
      421 tcgggtagtt catgcccagt atcacaaaaa acatattcca tctgggggac tttgtcgcgc
      481 atgtacaggg caagtgccgt gctgtccttg ccaccagaaa gggagaggat atgcctacat
      541 gtctgcgggt caggaacgat tgtcttcttg gtcatgtttg cagttcctcc aagagtttga
      601 gcatcgcggc acgaatgact gcaggcgatg tcttctgagt tgtcttctgc aggtgttgct
      661 tcacgcttac aacaagagcc tcagcctctc cctgctgttc ttcggtcaat tcgatgttcg
      721 gatcatcgga aatcctcctc gtacacacag cctcacgaaa agacgctcct gcagctgcgg
      781 cggcgtcggg aaatcgttct gcatcagcgt cagtccagtt gcgaggaggt ttgctggcaa
      841 caagtgcaat aacggattca acccctgcag catctgccgc agactgagca atccgctgaa
      901 caaatgacag agtctgatga ttcgtcacat aaggctcaac cgcgacagca agttcacgca
      961 gctgctgcca tccggagtcc ccactactca agccacaagc attcagcaaa gagtcgcgcg
     1021 cccccgcaat tgttctcgga gttgcgtctg cccactcacg aaggctctgg ttcagaaggt
     1081 cgaagaatct gtccacttct tggggcgtat ggcttccttc cggaaaagcg ggcatgccca
     1141 atgcttccgg aagcatgcaa tacaaaaaca cctcgggtga cttcgcgttc cgaaatgctt
     1201 cacgaagcgc cacaacggat tccgaaagac gctgcgtgtt ccaggcatac tcaggaagac
     1261 tcttcaccat agcaaacaga gcacgaacaa tcggcacagt cgccgaagca acaccaagcc
     1321 cacgcgccaa ccgattcacc accagtgccc gagacccagc aattctgctt cctcccaccg
     1381 caaacagctc gggtctccgc atcaggacct cgaaggctgt taccgtgatc tcaggaagaa
     1441 aagttccttc gcgatacaga gacgtttcat cggaatgcgc cagcaaaaag gcacacagaa
     1501 gcaccggatg caagcccggc agaacaccga aaggaggttc ggcaatctgc gcaaaaagcc
     1561 ggtccaacgg aaccggcccc gactttgacc caaatatggc atccgagagg aaactccaca
     1621 aggcgctcag attcgaaggg tcagaagagt ctggatcgca aaaccgccag gcaccttcat
     1681 caaaccggtg aagtcccgaa acgcgcagca ccgactcgta agcacttctc tcgggcggat
     1741 acccctctat gccaagcgct tcctcgctgt gccgtgtcag cattcgctcg atgaggttcc
     1801 gtcgggccgc tgcggcggca gatgatatca gcctccggct gatgagttca ttgcgtattc
     1861 ttggcgcgct cggataaacc tgatcgcaga catccgaaag gagttgagac actccagcgg
     1921 gagaactcac aggaatacaa cgcccatcat gaatccagag acaaccgctc ccaatcggtt
     1981 catcccttgg atccatgaga ctgtccacgt tgcgccaaag aatctgctcc accgaagcaa
     2041 tacggagcga cagctctttc ctggcaacac ggtcatctcg aatctcagga gtattctccc
     2101 aagcccatcg caaagcccct atctcagtga caatcgcccg caattctcca atgaactggg
     2161 gaatggcaaa gagtgtattc ctgcaggctt tggagacaag tgcaatttca cggaaacgct
     2221 ccgtaagcat atcggattct ccaaggcaga caacaaccgt tcccccatct ttaggaacac
     2281 tcaagcactg ttcaatggca tctggatcat caagatactc gacagaaaaa taccgcaaag
     2341 ccccggtctc gaagctgtgt cgccttgcga ccaagggtct ctttggaaga tattgcctca
     2401 accgatcggc gagattcaac ccatggcgga tctgcctctc cccttcagcg atacgttcat
     2461 caatgtcaac gtcactgcct tcccaaattc ggtacgttcg attgaactta cggaaagtca
     2521 atacggactg ctcccttaga aactgcaaca cgtcgatcag atcgctgctc gggacggaac
     2581 agtccgtaac agcaagggaa atgacctctt cggttgcgtt cagatgacaa aactcaccca
     2641 acgcattcag aactcctatc gtcttaacca cctcgcgatg aagcgacgag agtccttctc
     2701 tccgctccaa aacgtcggca gcctccatcc agcgcaaagc ctgaggatgg cggtagagtc
     2761 ctgctccaaa gttctttgta aaatagtcaa aaaggtggct gagccgaaca accggtgggc
     2821 agtccttctc aagcggcttc gttcggatga actcctgaaa accatagggc tccaaactgc
     2881 tcaggtagga aaagagagac cgctcgttct gagcgaaccg ccgaaacagg aatggaaggg
     2941 cgactaatgt gactggatga atcggatacg ctcctcgtga tgcggtctcg aaatcagaat
     3001 ccttcatgcc cggcggagca atgccggctt tcaacgcacc cagaactatc ttgtccagaa
     3061 accgtgccgg aatctgtgct gtcgcatctg gcgaccattc gatggctttg gcgatcatcc
     3121 gaatgacttg ctcaggaggt tccagaaatg caatatcctc aaaccgacct tgaatcttcg
     3181 cccattcgcg cctggtcgca agatcaaggt gttgggcata gtcctcaaaa gactgatgca
     3241 ggatgccgac caagaggatc ggacaacggc cactccggga tgcctgttcc gcaatctcct
     3301 gcaggacaaa aacatctccc ttctgtggat accgagctgc atactcaaaa agcttcccca
     3361 gttcgtcaac aacaagcagg actccccgat agcctgcatt agttgccttt gccgaaagcg
     3421 aaaccaacgt attggtcacc tgccttgtat cccatggctg accatttcgc tgtgaatcaa
     3481 gtttctccga gatttctctc acacagtttt tcagactgtt tactcgagta gctgctgccg
     3541 aggcaatgat cccttcagca atacatcttg aagcaggggt ccgccttgcg gtagcaagaa
     3601 tcggaagaaa ccctttcgag ccgttatcga aaatccccag ttcgagtaga tgagcatgca
     3661 gctgtggatc acactctagc aactggcttc gcacttgagt gtccgaagca ctcgaagaac
     3721 ataggagtcg tgtaaggaag acggcaaacg cagacttgcc aacaccataa ggacccgtca
     3781 ctgtccagga ccggcatgag gaaccctcgc tcataccctc agccagacgc ctgagagctt
     3841 cccgtgcggt cggtgtcagg atatagtccc cattctgggt tgaattgcag aaatccttct
     3901 ccagattgat tgagcgtctg aagcgtcctt tgacctcaag tacatcgcag agcatcatgg
     3961 ctgtagaact cacttccgac ttcctccgag gtaataatca ccaagcaccc gcagagggtc
     4021 aatagagcgc ctgcgataga tttgtttcaa cccagccgtt tcatccaagg caatgctgtc
     4081 ctccaacgaa tcttccaatt cctcaaggta ctcgatcagg ctgttctcat ccaacttgaa
     4141 gacctgtccg ggacttccct ctccatagag gcattcctga aggctcatcg tgttgcgacc
     4201 ggcacgcgtc ttctcaaaat gctcgtgcaa tgcaaatgca aaaaccgctg cgggcagaga
     4261 gggcttcgga ccaatggcaa atcggtaaag atccccgtct ggagcaaatt ggataagatt
     4321 caactgaaga agcggacaac tataggtctc ttcaacaact gcctgtttct tgctgccgga
     4381 gctcgcaacg tatgtccgga tgaggcagtc tacatcacgc atgattgatg aatccccaac
     4441 cttcatgccc tccctttcgg caaagctacg gacgaagtcc gtgatctccc gccttgtgaa
     4501 gtcaggtctg ttaaag
//
LOCUS       DAOQUC010000128         4504 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_21464_length_4504_cov_3.503259, whole genome shotgun sequence.
ACCESSION   DAOQUC010000128 DAOQUC010000000
VERSION     DAOQUC010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4504
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21464_length_4504_cov_3.503259"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..455)
                     /locus_tag="PKH07_03400"
     CDS             complement(<1..455)
                     /locus_tag="PKH07_03400"
                     /inference="COORDINATES: protein motif:HMM:NF016115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HNT34023.1"
                     /translation="MNDHNWLDITVPIAENMAYWQGDPPVRVERLSRIEEGDSCNMTL
                     LSFTAHTGTHLDAPLHFLRSGCSMDSMPPSLMVGQAHVLSTGDTKHISADQLQRVNIR
                     SGERILFRTSNSNTPWLTEKFRTDYVHLTPSAARYLADKNVALVGIDYLS"
     gene            complement(991..1066)
                     /locus_tag="PKH07_03405"
     tRNA            complement(991..1066)
                     /locus_tag="PKH07_03405"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1031..1033),aa:Ala,seq:ggc)
     gene            complement(1076..1462)
                     /gene="acpS"
                     /locus_tag="PKH07_03410"
     CDS             complement(1076..1462)
                     /gene="acpS"
                     /locus_tag="PKH07_03410"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013579171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HNT34024.1"
                     /translation="MRIAGVGLDLVDVERVRNCLERYGERFKGRVFTEKEVAYCDRYR
                     YSYENYASRFAAKEATLKALGIGKSRGVAWTDVEVTRNPGEAPKILLHGKAAEIAENL
                     AIVRLHVSLAHERDIAQAIVIAEQNE"
     gene            1690..3198
                     /locus_tag="PKH07_03415"
     CDS             1690..3198
                     /locus_tag="PKH07_03415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34025.1"
                     /translation="MTLLRFSLCLSLGVCLWMGVSTRAEAQRSLNDLTGAWQLFVDDY
                     LVESSTNVKRVYHAFEKHAGNPVMKADKPWEGRNIYVYGTVLPEESGKGYRMWYHGLP
                     EKKGDDPYRLLYATSKDGVSWEKPSLGIIEYEGSKDNNIYIRRNPRDHIQSVIHTPWE
                     SSPQRQYMMMNWSSDGYYLTWSPDGIHWTDVPNNPVLTKGGDVGNFCWDPRTKQYCGY
                     VKLGSVVRDLKRRSVGFTSTSDPLQWPDPELIIEPDEIDDRWATPPARTQFYGFCAFP
                     YETMYLGFLWLYRSYPTPADPEGYMDGVIYVELASSRDGKHWDRIAPAEDGLRPAILP
                     LGAEGTWDSGMICTTNHPLVESNQIKLYYGGTLGTHYSASKDWVSAIGLATMRKDGFA
                     SLDAKGAIGVVTTKSLVGYAGKLYANFRSHSPAGCLRVEVLDEEGATVPGYGLADCVA
                     LTGDCPNSTVRWNGSSSLPATTSPIRLRFHVSDGSLYSFMAGSEVRLAEAPR"
     gene            3406..>4504
                     /gene="aspS"
                     /locus_tag="PKH07_03420"
     CDS             3406..>4504
                     /gene="aspS"
                     /locus_tag="PKH07_03420"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011877131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HNT34026.1"
                     /translation="MERTYAGTLRRQDIGSTVKLSGWAWRIRDHGGVLFIDLRDTSGV
                     CQVVCNPEKSMESFQVAEKVRNEFVLSVEGLVEARTEDTINPKMETGEIEVMASRLEI
                     LNVADPLPMIIEDDLDASEEVRLKYRYLDLRRPEMQRRFKLRHQAYQITRRYLDRQGF
                     LEIETPVLAKSTPEGARDFLVPCRFSPGTFYALPQSPQLFKQLFMVSGLDRYFQIVKC
                     FRDEDLRGDRQQEFTQIDLEMSFSGVDEVCTLVEGLITTILRETIGVEIPVPLRRLPY
                     SEAMSRFGSDKPDTRFGLELGSINDIAGRCEFKVFRDVVDSKGLVSGINLKGCGGTFS
                     RKEIDDLIAFCQQFGAKGMAYFKVTEKGLEGS"
BASE COUNT         1050 a         1080 c         1269 g         1105 t
ORIGIN      
        1 gagagatagt ctatgccgac caatgcgacg ttcttatctg caagataccg ggctgcggat
       61 ggggtcagat ggacatagtc ggttcgaaac ttctctgtca accagggagt gttcgagtta
      121 gaggttctga agagaatgcg ctcaccggag cggatattca ctctctgcaa ctgatccgca
      181 gagatatgct tggtatctcc agttgagagc acatgtgcct gtccaaccat caatgacggg
      241 ggcattgaat ccatgctgca accagagcgg agaaaatgca gaggcgcgtc cagatgcgtt
      301 cccgtgtgcg cagtaaagga caagagcgtc atgttgcacg agtctccctc ttcaatacgc
      361 gacagacgct ccacgcgcac aggcggatcg ccttgccaat acgccatgtt ttccgcgata
      421 gggacggtaa tgtccagcca attgtgatcg ttcatatgac ctcaaagcgc cctgaattgg
      481 ccacgccatg ccagcagcat attcagatat gacgcggaat ccgtcaagtc gaggactggc
      541 ttgcacttta gggacatatt gactttgcta aaacacaacg ggcttcggaa ggtgggcatc
      601 cagctcgacg ttttgaccca tcgcgggtct tctgccttca gaacggcgca gtgacttcac
      661 tcgagaagcg aagcagaact accgaaacgc cgggagccgt tttgggtacg cggtatcgtt
      721 cccgaattgt tcctgatcac gggctggatg tccttgttac aactgcaggg aagacgctgg
      781 ttgagtctca ggagtggaga accttggttc cgagctaagt ggtctgtggc agcgaggtcc
      841 cgcagtcgtg aaagagaaac acagtccgca gccacgcttt cactcaatcc gggaaccgac
      901 ttctggcgaa atccgtggta cttgccacta acttgaaagg gaatcggcca ctgcgtccgt
      961 tcgcgagtgg ccgattttaa ggtctttttc tggtggagct gagggggatc gaacccctgg
     1021 ccttctgaat gccattcaga cgctctccca gctgagctac agccccaccg gtttgttatt
     1081 cgttctgttc ggcgatcact atcgcttgtg caatgtctct ttcgtgtgcc aacgaaacat
     1141 gaagccgaac tattgccaga ttttccgcaa tctccgccgc tttgccatga agcaatatct
     1201 tgggcgcttc acccggattt cgagtcactt cgacatccgt ccaagcaaca cctcgtgact
     1261 ttccgatgcc aagagctttt agtgttgctt ccttggctgc aaagcgcgaa gcataatttt
     1321 cgtaactata ccgataccga tcacagtatg caacttcttt ttcagtgaaa actcttcctt
     1381 tgaaccgttc gccatatctc tcaaggcagt ttcgaactcg ttctacgtca acaaggtcaa
     1441 gcccgacgcc agctatccgc atcagagaat ccttttctgt cctcgatcca atctgtgctg
     1501 agtgatattg aaatgagaga gatttgtcaa gttcaccaag gagtttgcag acgacgatga
     1561 aaacgcagat cgcatgaata aggacttgca gtgtgcggtc aaactccata agataccaac
     1621 gttccaatga cttttgctgt tttctgcgaa ggtcgggtct gaaagggctg ttcggtaaag
     1681 gagaaagata tgactctgtt acgattctct ctatgtctgt cgctgggtgt ttgtctgtgg
     1741 atgggagtca gcactcgggc agaggctcaa cggtcactca atgacctgac tggagcctgg
     1801 caacttttcg tagacgacta tcttgttgag agttccacga acgtcaagag agtctatcat
     1861 gcctttgaaa agcacgcagg caatccggtg atgaaggcag acaagccctg ggagggcagg
     1921 aacatctacg tctatgggac tgttcttcct gaggagtcgg gaaagggtta ccggatgtgg
     1981 tatcacggac tgccagagaa gaagggtgac gacccgtatc ggttgctgta cgccaccagc
     2041 aaggacggcg tgagttggga aaagccatcc ctgggcatca ttgaatacga aggttccaag
     2101 gacaacaaca tatacatccg ccgcaatccg agagaccaca tccagtcagt cattcatacg
     2161 ccgtgggagt catcacccca acggcagtat atgatgatga actggagcag tgatggttac
     2221 tacctcactt ggtctcccga tgggattcac tggacggatg ttccgaacaa tcccgtgttg
     2281 accaagggtg gcgatgtcgg gaatttctgc tgggatcctc gaacaaaaca gtattgcggt
     2341 tatgtgaagc tgggaagtgt cgtcagggat ctcaagaggc gttcggtcgg ttttacttca
     2401 acaagtgatc cacttcagtg gcctgatccg gagttgatta tcgaacctga tgaaattgat
     2461 gatcgctggg caaccccgcc ggcgcgtact caattctatg gtttctgcgc atttccctat
     2521 gagacgatgt atctgggttt tctgtggctc tatcgttcct atccgacccc tgctgatcct
     2581 gagggttaca tggatggggt gatctatgtg gagttggcaa gcagccggga tggcaagcat
     2641 tgggatcgga ttgcgcccgc tgaggatggc ttgagaccgg cgattctgcc cttgggagca
     2701 gagggtacct gggattctgg gatgatctgt accactaacc atcccctggt cgaaagcaac
     2761 cagatcaaac tctactatgg gggcaccctc ggtactcact attccgcttc caaagactgg
     2821 gtctccgcga tcggtcttgc aacgatgcgc aaagatggat ttgcttctct ggacgccaag
     2881 ggtgcgattg gtgtggtcac aaccaaatcg cttgttggat acgcagggaa actttacgcc
     2941 aatttccgga gtcatagtcc ggcaggctgc cttcgagtag aagttcttga tgaggaaggt
     3001 gccactgtgc caggatatgg cttggcggac tgtgttgccc tgacgggaga ctgcccgaac
     3061 agcacggttc gatggaacgg gagttcgagc cttccggcga caacttcacc gattcgtctc
     3121 cggttccatg tcagtgacgg ttctctctac tccttcatgg caggaagtga agtgagactg
     3181 gcagaagccc cgcgatgatc gagcggattt gacgcttttt tgaggtaaag acacctttcc
     3241 gcttgacaga atagactgcc cgtgaagaaa tacatccttc atagggcgaa tcgagtcccc
     3301 gtttctggca agaaggactc accccgacag agaacatgaa gggaagagaa ctccgcaaaa
     3361 ttgggttctc ttcttgaggt ttatcgaggc tggaggcagg tcatcttgga acggacttat
     3421 gcaggaacgc tgagaagaca ggatatcggc agcacggtca aactgagtgg ttgggcttgg
     3481 cggattcgag accacggtgg tgtgcttttt attgatttgc gggacacgag cggagtatgc
     3541 caggttgtct gcaatcccga gaagagtatg gaatcattcc aagtggcaga gaaggtcagg
     3601 aacgagtttg ttctaagtgt tgaaggcctt gttgaggcac gcacggaaga tacgatcaac
     3661 ccaaagatgg agacagggga gatcgaggtt atggcgagcc gcctggagat cctcaacgtg
     3721 gcggatccac tcccgatgat catcgaggat gatcttgatg cttccgagga agtcaggctg
     3781 aagtatcggt acctggattt gagacgtcct gagatgcaga ggcggttcaa gttgaggcac
     3841 caggcatacc agatcactcg aagatatctc gaccgacagg gttttctgga gatcgagacg
     3901 ccggtacttg ccaagagcac gcctgaaggg gcacgcgact ttcttgtgcc ctgtcgtttt
     3961 tcgccaggga cgttctacgc gttgcctcag tcaccgcagt tgttcaagca gctgttcatg
     4021 gtttccgggc ttgaccgata tttccagatt gtgaagtgct ttcgagatga agacctcagg
     4081 ggagatcggc agcaggagtt cactcagata gacttggaga tgtcgttctc tggagtggat
     4141 gaggtttgca ctctggttga ggggctcatc accactatcc tcagggagac tattggtgtc
     4201 gagattcccg ttcctttgcg caggcttccc tattctgagg cgatgtctcg atttggctct
     4261 gacaaacccg atacacgttt tggtctggaa cttggtagca tcaacgatat tgcaggaaga
     4321 tgtgagttca aggtctttcg cgatgttgta gattcaaagg ggctggtgtc tggtatcaac
     4381 ctgaagggtt gtggtgggac tttttcccga aaagagattg atgatctgat cgccttctgt
     4441 cagcagtttg gagccaaggg tatggcgtac ttcaaggtca ctgagaaggg gcttgaaggc
     4501 agta
//
LOCUS       DAOQUC010000129         4501 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_21489_length_4501_cov_2.998201, whole genome shotgun sequence.
ACCESSION   DAOQUC010000129 DAOQUC010000000
VERSION     DAOQUC010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4501
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21489_length_4501_cov_2.998201"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1557
                     /gene="metG"
                     /locus_tag="PKH07_03425"
     CDS             <1..1557
                     /gene="metG"
                     /locus_tag="PKH07_03425"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HNT34027.1"
                     /translation="LFICGSDDHGVPVCLTARQENSTPRQIVERYNTAQHKAFEGIGI
                     HFDLYGSTSSPLHHKMSSDFFLKVHAQGAITKKSSEQLYDAQAGMFLPDRYVKGTCHH
                     CNAPDALGDQCEACGKTIDPLLLRDPVSVISGTRPEIRSTTHWFFQQSLFEERLSEWL
                     EGHAEWRPFVRNFAQGLVKQGLPDRAITRDLDWGVPVPLADDPDASGKVLYVWFDAPI
                     GYVSFTAEWCAQHGMDPDSYADWWRDPECKIIHFIGEDNIVFHAIIWPAMLMAEGTYQ
                     LPSNVVANSFLNIQFPNRPVEKISKSRGTAIWIDNYLEDYHPDLLRYYLTAIASETQR
                     TTFSLEEMVTRVNSELVDALGNFIYRTLSFTMRYFDGKVPEKGTPTETDLQHWSEIQA
                     LPERLSHEIESFRFRNALAELMSATRASNKYIDTKAPWKQRKEDLAGCATTMYYCIQT
                     TKALAVLMNPFLPFTSEKTLATLGLDPTDLDWDKATDPLPEGAPLQQPEIPFKKMELP
                     EDVTRLSRNE"
     gene            complement(1574..4351)
                     /locus_tag="PKH07_03430"
     CDS             complement(1574..4351)
                     /locus_tag="PKH07_03430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34028.1"
                     /translation="MSSALVMKLAGITFLLLLGTRPSVALGAGALVDHVEFDHTRTYS
                     GTGVQAVSFTWTFGDRTLPLSGVQLGSRDSRLHFKLRVREGRNLTLELREFRTADGQS
                     PAYLVCVGGKPVAFRCRRYVGTGSISAFIDLPDCEPKQSLRIEIVNRNDKPISFSEAY
                     LYEDLESFVVEKDFSQPMFVGPTIRHNDSVERLNEIRAMLPDTEQMKPMCLLAAVPVA
                     NLSPGDLQEWLDDALTRCAEADLPLELQVVTWWAGTPGGLDGKGGCWHDPEYQQINYV
                     PSEGRYFLAVPNRWASTPWLTVRHPRLNAFKQKQFQRAGQILRGLKERYQSDGKPFPV
                     LSIVIDNEVTYWGAGMPETPVGILADVNPAMVTAARQEGVILNPEDGFSDEEIAFLRR
                     SLRVYNRQMAQGVVEGLGNSDLRDLVYTHTWALGCVFDDPAEAYEAGVLKEVRMGGEW
                     NYTRAEQLAFTDLDREFGIPAAINIELGGQKTFCEEIPFAFAAGSHHASMFNADDELL
                     QVTFREIKEHGWPEYAPVPWRHHLLKLDFTTDEWMNYITGEDVHVQAIGSRDGYLLHG
                     KTLEQSNTCRMHLKAKELTSEGVFPKLWLKYRARSFVFQKVSDDAYLIIRAGKTPGSL
                     QQVERLTNASGWYHVDLSTLTEGEEALYLEFEFHPLGLVGWVGLFDLVFEVPWEEELL
                     LRCNRSYRADRLRAESQIAGWRADAAWTLQRCQEMGEKIPAKTLRQLKSLMRNHDYRQ
                     VHEQGLLALSKRRTETYLPIELPSPNRSERGQLRSAGGNSLRFSPYDEGYTYQSVVVS
                     ETADMTLVTNGETQTLQGLTGLQAGDDIEMTIHDNQVTCLVAHRSEATGVVVNASPAT
                     AFSLPTIQIEGGALLDIDSRCSITDRTGEAQHGSCPLVTDKRLFEPGDRVTMRWNPFR
                     KRVVEIRPANP"
BASE COUNT         1051 a         1295 c         1154 g         1001 t
ORIGIN      
        1 cttttcatct gtggttccga tgaccacggc gttcccgtct gcctgacggc gcgccaggag
       61 aattccacgc cccgacagat cgttgaacgc tacaacactg cccagcataa agcctttgaa
      121 ggcatcggca tccatttcga cctctatggc agtaccagca gccccctgca tcataagatg
      181 agcagtgatt tcttcctcaa agtccatgca cagggcgcta tcacgaagaa aagcagcgag
      241 cagttgtacg acgcgcaggc aggcatgttc cttccggatc gctatgtcaa aggcacctgc
      301 caccactgca atgctccaga tgcactcgga gaccagtgcg aggcgtgcgg aaagaccatt
      361 gatccccttc tgctcaggga tcccgtgagt gttatctcag gcactcgacc ggaaatccgc
      421 tcgacgactc attggttctt tcagcagtct ctctttgagg aaagactgag cgagtggttg
      481 gagggacatg ccgaatggag accctttgtc aggaacttcg cccaagggct tgtcaagcaa
      541 ggacttcccg atcgggcaat tacccgtgac ctcgattggg gcgtcccggt gcctctggca
      601 gacgatcctg acgcttcggg caaagttctc tatgtgtggt ttgatgcccc catcggctat
      661 gtctccttca ctgccgaatg gtgtgcccaa cacggcatgg atccggattc ctatgcggac
      721 tggtggagag atcctgagtg caaaatcatt cacttcatcg gtgaagacaa catcgttttc
      781 cacgcgatca tctggccggc aatgttgatg gccgaaggca cctatcagct gccttccaac
      841 gtcgtggcga acagtttctt gaacatccag tttcccaacc gacccgttga gaagatttcc
      901 aagagtcgcg gaacggcaat ctggattgac aattaccttg aggactatca tcccgatttg
      961 cttcggtatt acctgaccgc cattgcgtcc gagacccaac gaaccacatt cagtctggaa
     1021 gagatggtca cgcgggtcaa cagtgagttg gtagatgctc ttggcaattt catctaccga
     1081 accttgagtt tcaccatgcg ttattttgac ggaaaggttc cggaaaaggg aactccaaca
     1141 gagaccgacc tgcaacactg gtcggagatc caggcactac ccgaacggct ctcccacgag
     1201 atcgagtcct ttcgttttcg caacgcgctt gccgaactga tgagcgcaac gcgggcttcc
     1261 aacaagtaca ttgacaccaa ggcaccttgg aagcagcgga aagaagacct ggcaggctgt
     1321 gcgacaacga tgtactactg catccagacg accaaggcgc tcgcagtctt gatgaatcct
     1381 tttctgccct tcacttccga gaaaacactc gcaacactcg gtttggatcc aacagacttg
     1441 gactgggaca aggcgaccga tccgcttccc gaaggagcgc cactgcaaca accggaaatc
     1501 ccgtttaaga agatggaact gccggaagac gtgacacggc tgtcacgaaa cgaatgaccg
     1561 tatactgaat cggtcaaggg ttcgctggac gaatctcgac gactctcttg cgaaaaggat
     1621 tccatcgcat ggtgaccctg tctccgggct cgaaaaggcg cttatccgtc accagcggac
     1681 aggagccgtg ctgagcctct cccgttcgat ccgtaataga gcatctgctg tcaatgtcca
     1741 acagcgcccc accttcgatt tgaatcgtcg gcagagaaaa ggcagttgcg ggcgaggcat
     1801 tcacaaccac tcccgtagct tccgatcggt gtgcaaccag acaagtcacc tggttgtcat
     1861 gaatggtcat ctcgatatcg tctcctgcct ggagtcccgt cagaccctgt agcgtctgtg
     1921 tctcgccatt tgtgaccagg gtcatatccg ccgtctcaga gacaacaacg ctctgatagg
     1981 tgtacccctc gtcataggga ctgaaacgga gcgagttacc accggccgaa cgcagttgtc
     2041 ctcgctcact tctgttcggt gacggcagtt caatgggaag atacgtctcc gtccggcgct
     2101 tcgaaagagc caagagtccc tgttcatgca cttggcggta gtcgtggttg cgcataagtg
     2161 atttcagttg tcgcagcgtc ttggcaggaa tcttctctcc catctcctgg caacgctgga
     2221 gagtccacgc agcatcagca cgccatcccg caatctgact ctccgcacgc aagcggtcag
     2281 cacgatagct tcgattgcag cgaagcaata actcctcttc ccagggcact tcaaagacca
     2341 gatcgaacag cccaacccat ccgacaagtc ccaaagggtg aaactcgaat tccaggtaca
     2401 aggcttcctc cccttccgtc agtgtggaga ggtccacatg gtaccaaccg gatgcattgg
     2461 tcaaccgctc cacctgctga aggctgccgg gcgtctttcc ggcacggata atcagatagg
     2521 catcatcgga gactttctga aagacgaaag atcgggcacg atacttgagc caaagtttgg
     2581 gaaacacccc ttctgaagtg agttccttcg ctttgaggtg cattcgacag gtgttcgact
     2641 gctccagagt ctttccatgc aacaggtatc cgtcgcggga tccgatggcc tgcacatgga
     2701 cgtcttctcc cgtgatgtaa ttcatccact cgtctgtcgt aaagtccagt ttcagcagat
     2761 gatgacgcca gggaaccggt gcgtactcag gccatccgtg ttccttgatc tcacgaaagg
     2821 tgacttgaag cagttcatcg tcggcgttga acattgaggc atgatggctg cccgctgcaa
     2881 aggcaaaagg aatctcttcg cagaaggtct tctgtccacc caattcgatg ttgatggctg
     2941 cgggaatccc gaactcccga tccaaatcgg tgaaggcaag ttgttcggct cgggtgtaat
     3001 tccattcgcc acccatgcgc acttccttca agactcccgc ttcatacgct tcggcgggat
     3061 cgtcaaagac gcagccgagt gcccaggtgt gcgtgtagac aagatcccgc aggtcactgt
     3121 tacccaaacc ttcgacaacc ccttgcgcca tttggcgatt gtatactcga aggctgcgac
     3181 gaagaaaggc gatctcctca tcggagaaac cgtcctcagg attcagtatc acaccttcct
     3241 gtctggcggc agtcaccatg gctggattca catccgcgag aatcccaacc ggtgtctccg
     3301 gcattccagc gccccagtaa gtgacctcgt tgtcaatgac gatgctcaag actgggaagg
     3361 gcttcccatc gctttgatag cgttccttta gtccacgaag aatctgacct gctcgctgaa
     3421 actgcttctg cttgaaggcg ttcaggcggg gatgacgaac ggtcagccat ggagtgctcg
     3481 cccaccggtt gggtaccgca agaaaatacc ttccttcgct tggaacatag ttgatttgct
     3541 gatattcggg gtcgtgccag catccccctt ttccatcaag accaccagga gtcccagccc
     3601 accaggtgac tacctgaagt tcaaggggaa gatcggcctc ggcacatcgg gtcagtgcgt
     3661 cgtccagcca ttcctgcaag tcccctggcg acagattcgc cactggaacc gccgcgagaa
     3721 gacacatggg cttcatctgt tctgtgtccg gcagcatggc acggatttcg ttcagcctct
     3781 caacgctgtc attgtgacgg atggtgggtc caacaaacat cggttgggaa aagtccttct
     3841 ccacaacgaa ggactccaag tcttcgtaga gatacgcttc cgagaaagaa atcggcttgt
     3901 cgttgcggtt gacgatctca attctcagag actgctttgg ctcacagtca ggcagatcaa
     3961 tgaatgccga gatcgaccct gttccaacat agcgacgaca gcgaaaagca actggttttc
     4021 caccgacaca gaccagatat gcaggagact gcccgtctgc tgtgcggaac tctcggagct
     4081 ctaaggtaag gttcctgcct tctcgaactc tgagtttgaa gtgaagtctg ctgtcacggc
     4141 ttccaagttg gactccggaa agaggaagcg tccgatcacc aaatgtccac gtaaaggaaa
     4201 ccgcctggac tcctgtgccg gaatatgtcc tggtgtggtc aaactcgaca tggtctacga
     4261 gcgcaccagc cccaagtgcc acgctgggtc tagtccccag cagaagcagg aaagtgatgc
     4321 ccgcaagttt cataacaagt gcactgctca tcgtcgccac ctctcctgga aacccctctg
     4381 agttacccca ctggaacttc aacctgccaa catttgagat agatgtcaat cagtattgac
     4441 gcttagacgt actggctcag ttacacaagg gcggagactg tcattccgaa cgactgaggc
     4501 t
//
LOCUS       DAOQUC010000130         4500 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_21491_length_4500_cov_3.749381, whole genome shotgun sequence.
ACCESSION   DAOQUC010000130 DAOQUC010000000
VERSION     DAOQUC010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4500
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21491_length_4500_cov_3.749381"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>4500)
                     /locus_tag="PKH07_03435"
     CDS             complement(<1..>4500)
                     /locus_tag="PKH07_03435"
                     /inference="COORDINATES: protein motif:HMM:NF013175.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34029.1"
                     /translation="FNFYSLINEGGVEERPALAHAEIQKTSENALCVTHFAGSNALGL
                     LINAVLEGDAEDMADQMNLRGDRYIPPAGEGYGGYCVPKDGLFLAFVLSLSNEVKLRQ
                     MGIPEHLHQGIKVLAKKAILKRQDFGTDFEWQDWAARELLQQNHLKEYFDLREDGLLV
                     FNITKIATTIQHLGEPWSEVASGSSLLSNLAARWSVEHMIINAEQVNRFMTFYKAWLI
                     YEALAEARRFNPNAPADNQARIALTAEYKPVQDVRFSTGLRLFEMFANTYEHLMHALD
                     EEGQNLAHLMLKGFDPDTEHPIGRRAVQEVVQKLGVASKEPASIQRLIESFPKHDSPA
                     DIVVTSVTLSSTKDMLFYTSDTRLDQVADRVKVKLGDFGLSEEQITANCQAFGGNLRA
                     WAGLRQIPAEQLERIVSDIGGEVHALVLKLRGPGRNYEKDIQGIDVLNTGIPFPELLQ
                     LLDNPPKLVRLMLMGNPNSALGIADGTAGRNRRSLTYRDVQNFLAACEKEGRRGIYKA
                     VGLGSNNVERLRADMHEKRDRALRLCTALEAVVQAQGSRETTEAIEKAQALYTDIIDR
                     ILLADEPGKAIREEERLKRYKKWKPGDTCISEALARIRTGLPLQELDIATWLGGLGGM
                     FVVMGEPQKQIDERFELIGKAVSLVGQWAKANKVASTFTGHRATKADIADAVKHLVRP
                     KFVPEVQQFAQEMLVESSSIAVEVAAIEALERRQALRARAARAQAFGQREEGFLSVYD
                     AQQKASSEEYARSAKDEMASILSLCKAQMSLDGQVDESVSEQCHRAFGRFLAYTRNYL
                     VALGRRAYAGGSDEDLARQAELEAHAAKCFSGREIIMEDWKLLAGGYENLGDIARLAE
                     KVKDNKDFLMEVAQGVEFFYVTLALAQTLEFALQSEQEVDVRLFWKRAMDFFAETIND
                     HFYPYTPWAFSRGTGFAEYEGEELYGLANEHHAWLYEYLYTLLTSRTDYAALESVEQD
                     ALIGNFTGQNMVVPIGGDAAETRERRWRAYNQLREVVFLRGDGFQAPVVFPEFDPDII
                     EAQRRVNLVFLYPVGRTHISRAFREGPTLNKQLRDQGEPGLNLIVTRHGELTALPVAT
                     RECLMVSDGHLYISEQEYIQALIRHKGMTPEQARDEADKQRQEGRLTDKGIRAAVRFT
                     QSGQRKPIVAAAVFPFHGHPMYEDGQLESAGLPATVQSVIFTDITYDKSLYPDIYDQK
                     SGVRLPEEIDWKREYGKGKDEKSIMRAIQDGVKGTSYPGISAFSKKCPILLIKGAAES
                     GARNLKVFDLTGSRAEDVQAAVQFVYDVGKKQNVVIQEAVLTSPEFWASPELLDQFVE
                     RQILEWNRPVNRDKYPRSQVYGSLRVVASSSGPDCPYDTAFLIGLISLQVATNVGRGG
                     TLEPLMERYIQEEHREAILEGLKGEVPKVMNALARYAATYKGEFEKQHGRPVLSDLRG
                     VPYSWPAYMMLDYLVAPVFVRPGKLVDVETRYDDRGRRIGSKVILEDAEGRFEGKIKD
                     WQFIHL"
BASE COUNT          989 a         1314 c         1160 g         1037 t
ORIGIN      
        1 cagatggatg aactgccagt ccttgatctt gccctcgaag cggccttcgg cgtcttcaag
       61 gatgactttc gaaccgatgc gtcgtcctct gtcgtcgtat cgggtctcga catccacaag
      121 tttgccaggt cttacgaata ccggtgcgac aagataatcc aacatcatgt acgcaggcca
      181 gctgtacgga acacctcgaa gatccgagag aacaggtcgg ccatgttgtt tctcgaattc
      241 ccctttatag gttgcagcat agcgtgccag agcattcatg accttgggca cttcgccctt
      301 caagccttca agaattgcct cccgatgctc ttcctgaatg tagcgctcca tcaagggttc
      361 cagggttcca ccacgtccga cgttcgtggc aacctgaaga ctgatcaggc caatcagaaa
      421 tgcggtatca taggggcagt cgggtccaga agaggaagca acaacgcgca gagaaccgta
      481 aacctgggaa cggggatact tgtcccgatt caccggacga ttccattcaa gaatctgccg
      541 ttcgacaaac tgatccagaa gttccggact tgcccaaaac tcaggagagg tcaacacggc
      601 ttcctggatg acaacgttct gtttcttacc aacatcgtag acaaactgaa cggcagcctg
      661 aacgtcttcc gctcgtgatc cagtcaggtc gaacactttc agatttctgg cacccgactc
      721 ggctgcgccc ttgataagaa ggatgggaca cttctttgaa aatgcactga tgccaggata
      781 ggatgtgcct ttgacaccgt cctgaatggc tcgcatgatg gacttctcat ccttaccctt
      841 gccatactca cgcttccagt caatttcctc aggcagccga acaccggact tctgatcgta
      901 gatgtctgga tagagtgact tgtcataggt gatgtcggtg aagatgacac tctgaaccgt
      961 agcgggaagt cccgcggatt ccagttggcc gtcttcgtac attggatgtc cgtggaacgg
     1021 aaatactgct gcggcaacga tgggcttccg ctggccgctc tgcgtgaatc gaaccgcagc
     1081 acggatgccc ttgtcggtca gtcggccttc ctgtctctgt ttgtccgctt catcgcgcgc
     1141 ctgctcggga gtcatcccct tgtgtctgat cagggcttgg atgtactcct gctctgaaat
     1201 gtacagatga ccgtcagaaa ccatcaggca ttcccgagtc gctacgggca acgcagtcag
     1261 ttccccatgt cgggtgacaa tcaggttgag tccaggctcg ccctgatctc tcaactgctt
     1321 gttgagtgtg gggccttcac ggaaggcacg ggagatatga gttcgaccca ccggataaag
     1381 gaacacgaga ttcacgcggc gctgcgcttc aatgatatcg gggtcaaact cagggaacac
     1441 aacaggtgct tggaacccgt caccgcgcag gaacaccact tcacgcaact ggttgtaggc
     1501 tctccagcgt cgttcccgag tctcggcggc atcaccacca atgggaacca ccatattctg
     1561 accggtgaag ttgccgatca aggcatcttg ttcgaccgat tcaagagcgg cgtaatccgt
     1621 cctggaggtg agcagagtgt agaggtactc atacagccag gcgtggtgct cgttggccaa
     1681 accgtacagt tcctcacctt catattcggc aaatcccgtt cctctggaaa acgcccaagg
     1741 agtgtaggga tagaaatgat cgttgatggt ctcggcgaag aagtccatcg cgcgtttcca
     1801 gaagagtcga acgtccactt cctgttcgga ctgaagcgcg aactccaacg tctgagcgag
     1861 cgccaacgtc acatagaaga actcgacgcc ttgagccact tccatcagaa agtccttgtt
     1921 gtccttaacc ttctccgcca ggcgtgcaat gtcgccgagg ttctcatagc caccggccag
     1981 aagcttccag tcttccatga tgatctcccg accggagaaa cactttgctg catgggcttc
     2041 cagctccgcc tgccgtgcga gatcttcatc agatccgcca gcgtacgccc tgcgtcccag
     2101 agcaaccagg tagttgcgcg tgtaggcaag gaaccttcca aaggcacggt ggcattgctc
     2161 ggacacagat tcgtccacct gcccgtcgag gctcatctgc gccttgcata gactcaggat
     2221 ggatgccatc tcgtccttgg cactgcgcgc atactcctca gaggatgcct tctgctgtgc
     2281 atcatagacg ctcaggaagc cttcctctcg ctggccgaag gcctgtgccc gcgcggctcg
     2341 tgcgcgaagt gcctgacggc gctccaatgc ttcgatggca gccacttcga ccgctatact
     2401 cgaagactca acaagcatct cctgcgcgaa ttgctgcact tcggggacaa acttcggacg
     2461 aacgagatgt ttaacggcat cggcaatgtc cgccttggtc gcccgatgac cggtgaacgt
     2521 ggaggcaacc ttgttggcct ttgcccattg cccgacaaga ctcactgcct tcccaatcag
     2581 ttcaaagcgc tcatcaatct gcttctgggg ttcacccatg acaacaaaca taccccccag
     2641 tccaccgagc cacgtggcaa tatccagttc ctggagcgga agacctgttc gaatgcgcgc
     2701 caaggcctca ctgatgcagg tatcaccagg cttccacttc ttgtagcgct tcagacgctc
     2761 ctcttctcga atggctttgc ctggctcatc agcgagcaga attctgtcta tgatatcggt
     2821 gtagagcgct tgagccttct caatggcctc tgtcgtttca cgcgaacctt gtgcctgtac
     2881 cacggcctca agtgcggtgc agagtctcaa cgccctgtcg cgtttctcgt gcatgtcggc
     2941 gcgcagtctc tcaacattgt tggagcccaa gccaactgcc ttgtagatac cacgcctgcc
     3001 ttctttctca caggccgcaa ggaagttctg tacgtcgcgg taggtcagcg aacgcctgtt
     3061 gcgtccggca gttccatccg cgattcccag agcgctgttg ggatttccca tcagcatcag
     3121 acgaaccagt ttcggggggt tgtccaacag ctgcaggagt tctggaaacg gaatgcctgt
     3181 gttgagaacg tctataccct gaatatcctt ttcatagttt cgacccggac cacggagttt
     3241 caacaccagc gcgtgaacct cgccgccgat atccgataca atgcgctcca gttgttccgc
     3301 ggggatttgt cgaagtcctg cccacgccct gaggttgccg ccaaaggcct gacagttggc
     3361 cgtgatctgc tcttcgctga gaccgaagtc ccccagcttc accttcacgc gatcggcaac
     3421 ctgatccaac cgtgtgtcac ttgtgtagaa gagcatgtct ttggtcgaag acagcgtcac
     3481 cgaggtcacc acaatatccg cagggctgtc atgcttcggg aacgattcaa tgagtctctg
     3541 aatgcttgcc ggctctttgg aagcgactcc gagtttctga accacttcct gaacagcacg
     3601 ccgtccaata ggatgctctg tgtccggatc gaaacctttg agcatgagat gggcaagatt
     3661 ctgcccctct tcatccaagg cgtgcatgag gtgttcgtag gtgttggcaa acatctcgaa
     3721 gagacgcagt ccagtcgaaa accggacatc ctgaacaggc ttgtattcag cagtcagagc
     3781 aatgcgtgcc tggttgtccg caggagcatt gggattgaag cgccgcgctt cggccaacgc
     3841 ctcgtagatc aaccacgcct tgtagaaggt catgaaccgg ttgacctgct cggcgttgat
     3901 gatcatgtgc tccacactcc atcgcgccgc cagattcgag agaagcgatg atccagaagc
     3961 cacttcagac cacggctcac ccaagtgctg tatggttgtg gcgatcttgg tgatgttgaa
     4021 aaccagcaac ccgtcttccc tgagatcgaa atattctttg aggtggttct gttgcagcaa
     4081 ctcacgcgct gcccagtcct gccattcgaa atctgttccg aaatcctgcc tcttcagaat
     4141 ggccttcttg gccaaaacct tgatcccctg atgcagatgc tccggaatgc ccatctggcg
     4201 gagtttcact tcgtttgaca acgaaagcac aaacgcaaga aacagaccgt ccttgggaac
     4261 gcagtatcca ccatagcctt caccggcagg gggaatgtag cggtcaccgc gaagattcat
     4321 ctggtcagcc atatcttccg catcgccttc aagcacggcg ttgatgagca ggccaagtgc
     4381 atttgagcca gcaaagtgtg tcacgcagag ggcattctca gacgtcttct gtatttccgc
     4441 atgggcaaga gctggacgct cttcaactcc cccttcattg atcaggctgt agaaattgaa
//
LOCUS       DAOQUC010000131         4494 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_21530_length_4494_cov_3.706691, whole genome shotgun sequence.
ACCESSION   DAOQUC010000131 DAOQUC010000000
VERSION     DAOQUC010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4494
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21530_length_4494_cov_3.706691"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            239..1276
                     /gene="rlmN"
                     /locus_tag="PKH07_03440"
     CDS             239..1276
                     /gene="rlmN"
                     /locus_tag="PKH07_03440"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HNT34030.1"
                     /translation="MTTGIDTLGLSLDQWADLMTRWGAAPYHGKQIFSWLHQKSATSW
                     EVMTNLAQSLRERLSAEFGSPEPRIVGHLTNEEAHKFAIQWHDDSVVEAVAIRGQRGD
                     TGCISVQVGCRVQCPYCASGKKGFVRDLTAGEIVAQVLVLRRACPGLDHLVFMGMGEP
                     FHNYDNLMHAIGIINTPSGIGIGARRVTISTAGVVPEIYRLANESLQVNLAVSLGAAQ
                     EDLRRKLIPMAKVYSFRQLMPALDAYLEKTHRRVSFEYTLLQGVNDRFAHADALAALV
                     RGRLIHVNLIPYNSVENLGFQAPRHQEIKAFRDRLEHLKVPVTIRQSRGSRIQAACGQ
                     LAARVQNERTT"
     gene            1273..2034
                     /locus_tag="PKH07_03445"
     CDS             1273..2034
                     /locus_tag="PKH07_03445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34031.1"
                     /translation="MMQSDQTPPPLRLTGTGVFLRYLHRVDEQLRQCVAFPLDLKMLP
                     MGLVSGGVFLFHTDISRPLSEQTGASERVSARKTVPLASSNDSMNGSPKTVACRVLAR
                     VGYGSKRGFLASHIVANSLNIYQWLDGIAGLPHSLMPVHCSESSRMLSWTIESGLRLR
                     HKATAIRFPFALKRSIDTFVLSVCGRTVYETEVRITGEFSLGLFTSTIQPRGVWQVCS
                     GLPVGALFIRNAELIVCPTRPALFAGAHPLAETGI"
     gene            2135..>4494
                     /locus_tag="PKH07_03450"
     CDS             2135..>4494
                     /locus_tag="PKH07_03450"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HNT34032.1"
                     /translation="MFLREVIELRAIFFLSLVCLLLALPVSSFASDTNVAAESERYQA
                     MWAYAWGTGFLNASQTDTMIQVARDNNVNSIFPQVRKTGDSYYISDTEPRASNISSGY
                     ADPLADILAKAHNTSEGRQRIEVHAWIVPYRVWVSSMGTPPTGHILKRHPEWKTKSYS
                     GTVSEEVIDPGIPEAQDYIVDVILEIIRKYNVDGVHYDYIRYLGRDQGYNEISVNRFN
                     ALYGRTGIPDSSDAQWSEFRRDQIRAVVRKAYAKVKQIRWDVKMSASTITWLPAPPSG
                     DFTRTRPYYDVFQDWPSMMSEGSLDLNCPMNYMRETDAAQKQGFRTWTDFTASVRAGR
                     HAIIGPGSYLNTIANNVIQMQYAIGVNGIVGTNIYRYGYSCLEQDEPASWEAIRAGVY
                     DERRNVPAASWLSSPTFGILCGTVKDSGGTALDGAVVTLSNGASGTIRTDGSGFYAFL
                     KLNPGVGYTAKAQYGSVEMTKVFLVEAGKVGTLDFTLTVPTATPSHTPTITPTPTQTP
                     TSTPTNTITLTATSSSTPTQTPTRDPSLPTETPTSTVPAAITATPTRTSTRTPTFTAT
                     RTYTPTMTSTRTPTATNSPTRTPTFTATRTPTWDPRTPTYTPTHTPTATATPTATPYT
                     LIVDNADADCQFTSGWTVSTDWSGYFREDALWIDKGTGSETAHFTFHVPNAGKYSLYE
                     WHPSVYSGVADAQLTVSHAKGNTPIFVRQNAYPSQWNFLGLYPFARGSYTMTLSNKAG
                     TLGTTVVADALKLVVYRLTGDLNGDHTVNERDLLLLIKAFHTKDAIA"
BASE COUNT         1041 a         1269 c         1194 g          990 t
ORIGIN      
        1 acttggagaa cacgctccac aatgccgaag tggttcgcga gaaacttctt cgagatggca
       61 cattccaagt ggaagtggcg ctcgatgtgg cgcggcttcc ccgaaaggtt tccaaggcag
      121 cgcaggcgca attgggcgca cgctatgttg tgaccgcgac aggacgaccc gagtagtctt
      181 tcgtcacggt ctgcactctg gcacccaaac gaaacagtga ctaacaaccg aggacttcat
      241 gaccacaggc attgacacgc tgggactcag tctcgatcaa tgggcggatc tgatgacccg
      301 atggggagca gcgccttacc acggaaaaca aatcttcagc tggctccacc agaaatccgc
      361 cacgtcgtgg gaggtgatga ccaacctcgc tcagtctctt cgggaacgac tctctgctga
      421 attcggatct ccggaaccgc gcattgtagg gcacttgacc aatgaagaag cgcacaagtt
      481 cgctatccaa tggcatgatg attcggtggt ggaagcggtg gccattcgtg gccagcgagg
      541 tgacacaggt tgcatctccg tccaggttgg ctgtcgggtt caatgtccct attgcgcttc
      601 ggggaagaag ggatttgtga gagatctgac tgcaggcgag atcgtggcgc aggttctggt
      661 tctgcggcgc gcgtgcccag ggttggatca tctggtcttc atgggcatgg gagaaccctt
      721 tcacaactat gacaacctga tgcacgccat cggcatcatc aacacgccaa gcggcatcgg
      781 cattggagcc aggagagtga cgatctccac cgccggcgtt gtgcccgaga tctaccgact
      841 ggcgaacgaa tcactccagg tcaatctcgc cgtttctctg ggagcagcgc aggaagatct
      901 gcgcagaaaa ttgattccga tggcaaaggt ttattcgttt cgtcagctca tgcccgcgct
      961 ggatgcctat cttgagaaaa cccatcggcg tgtctctttt gaatacacac ttctccaggg
     1021 cgtcaatgat cgcttcgccc atgcggatgc tcttgccgct ctggtgcggg gaaggctgat
     1081 tcatgtgaat ctcattcctt acaactccgt cgaaaatctg ggtttccaag cgcctcggca
     1141 tcaggagatt aaggccttcc gagacagact ggagcatctg aaggttccag ttaccattcg
     1201 gcaaagtcgt ggcagtcgaa ttcaggccgc ctgtggtcaa ctggcagccc gagtccaaaa
     1261 tgaacgaacg acatgatgca aagcgatcaa acacccccgc cacttcggtt gacaggaacg
     1321 ggcgtttttc tgcgctatct ccatcgtgtt gatgaacaac tccggcagtg tgttgctttt
     1381 cctctggact tgaagatgct cccgatgggc ttggtctcag gaggagtctt ccttttccat
     1441 acagacatct ccaggcctct ctctgaacag acaggggcat cagagcgggt atctgccaga
     1501 aagacagtac cccttgcctc gtccaacgat tccatgaatg gatctcccaa gaccgtcgcc
     1561 tgccgcgttt tagctcgtgt cggttacggc agtaaaaggg ggtttcttgc aagtcacatt
     1621 gttgccaaca gtctgaacat ctatcaatgg ctcgatggga ttgccggcct tccgcactcc
     1681 ttgatgccgg tacattgttc ggagtcttca cgcatgttga gttggacgat tgaatccggt
     1741 ctgcgtctga ggcacaaggc aacagcaata cgattcccct tcgcgctgaa gcgttcgata
     1801 gatacatttg tgttgagcgt ctgtggtcga acggtctatg agacagaggt tcggatcacg
     1861 ggagagttct ccctcggact tttcacaagt acaattcagc cacgaggagt ctggcaggtc
     1921 tgttcaggcc tgcctgtagg agcactcttc attcgcaacg cggagctcat cgtgtgtccc
     1981 acccgccctg cacttttcgc aggtgctcac ccgctcgctg agaccggaat ctgagtctcg
     2041 tgcaacgcgg gctgagaatt tgttcagttg acatcctgac gcaaagccat aagattttcg
     2101 atattgacgg aagacggatc gagaggagtc gtgcatgttc ttgcgagaag tgatcgagtt
     2161 gagggcgatc ttctttctgt ctcttgtgtg tctgttgttg gcattgcctg tcagctcttt
     2221 tgcttctgac accaatgtgg ctgcggagag cgaacgctat caggccatgt gggcctatgc
     2281 ctggggaacg ggttttctga atgcatcaca gacagacacg atgatccagg tggctcgcga
     2341 caacaacgtc aactcgatct ttcctcaggt tcgcaaaaca ggggattcct actacatctc
     2401 ggacacagaa cctcgcgcgt caaacatctc ttcaggctac gctgatcctc tggcagacat
     2461 tcttgccaag gcgcataaca cttccgaagg cagacagcgc atcgaagtcc atgcctggat
     2521 tgttccctat cgagtctggg tttccagtat gggaacacca ccgacgggac acattctcaa
     2581 acggcatccc gaatggaaga ccaagagtta ctcgggaacc gtctccgaag aagtaatcga
     2641 tccggggatt cctgaagcgc aggattacat tgttgatgtc atcctggaga tcatccgcaa
     2701 gtacaatgtg gatggcgtac actatgacta cattcgttat ctgggaaggg atcagggata
     2761 caacgagatc agcgtcaacc gtttcaacgc gctctatggt cgcaccggca tcccggattc
     2821 atcggatgct cagtggagtg aatttcggcg tgatcaaatc cgcgcggtgg ttcgtaaagc
     2881 ctatgcgaag gtgaaacaga tccgttggga tgtgaagatg tccgcatcaa cgatcacctg
     2941 gcttcccgcg ccgccctcgg gggatttcac gcgaacacgc ccctactatg acgtttttca
     3001 ggactggcct tcgatgatga gtgaaggcag tctcgatctc aactgcccga tgaactacat
     3061 gcgcgaaacc gacgcagcgc agaaacaggg attccgaacc tggacggatt tcaccgcaag
     3121 tgttcgagcc ggacggcatg cgatcatcgg tccgggcagt tacctgaaca ccatcgccaa
     3181 caatgtgatc cagatgcagt atgcgattgg agtcaatggc attgtcggca ccaacattta
     3241 tcgctacggc tacagctgtt tggagcagga tgagccggcg tcctgggaag ccatacgagc
     3301 gggagtctat gacgagaggc gaaatgttcc ggcggcgtcc tggcttagca gtccgacatt
     3361 tggcatcctc tgcgggacgg tgaaagactc aggcggcacg gctcttgatg gtgctgttgt
     3421 cacactcagc aatggtgcct ctggaacgat ccgaaccgat ggctccggat tctacgcttt
     3481 cctgaaactc aatccgggtg tgggatatac cgccaaagcc cagtatggtt cggtggaaat
     3541 gacgaaagtc tttctggttg aagcggggaa ggtgggcact ctggatttca cgctcactgt
     3601 tccaacggcg accccttcac acactcccac catcacaccc acgccaacac agacgccgac
     3661 gtctactcca actaacacga tcacgctaac ggcgacatct tcgtccactc cgactcagac
     3721 gccgacgaga gacccctctt tgccgacgga aacccccaca tccacggtgc ctgccgcaat
     3781 cacggcgaca ccgaccagaa ccagtacgcg aaccccaacc tttaccgcaa ctcgaacgta
     3841 cacccccaca atgacatcaa cgagaactcc cacagccacc aattctccca cgcgaacacc
     3901 gacgttcact gcgacaagaa ccccgacctg ggatccgaga actccgacct atacgccgac
     3961 ccacacgcca acggcaaccg cgacgccaac ggcgactccg tatacgctca ttgtggacaa
     4021 tgcagatgcc gactgtcagt tcacttcagg ttggacagtg agcaccgact ggtcagggta
     4081 ctttcgcgag gacgctctgt ggattgacaa aggaaccggc agcgaaacag cacatttcac
     4141 tttccacgtc ccgaatgccg gaaagtattc actctatgaa tggcatccat cggtctacag
     4201 tggcgtggcg gacgcccagt tgactgtttc tcatgcgaag ggcaacaccc cgatattcgt
     4261 tcgacagaat gcctatccca gtcagtggaa ctttctcggt ctctatcctt ttgcccgcgg
     4321 aagctacacc atgacgcttt ccaacaaggc aggcaccttg ggcacgaccg tagtggcaga
     4381 cgctctgaaa ctggtggtct acagactcac aggtgatctc aacggcgatc acaccgtcaa
     4441 cgaaagagat cttctcctgc tgatcaaggc gtttcacacc aaagatgcca tcgc
//
LOCUS       DAOQUC010000132         4486 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_21586_length_4486_cov_2.915595, whole genome shotgun sequence.
ACCESSION   DAOQUC010000132 DAOQUC010000000
VERSION     DAOQUC010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4486)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4486)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4486
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21586_length_4486_cov_2.915595"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1244)
                     /locus_tag="PKH07_03455"
     CDS             complement(<1..1244)
                     /locus_tag="PKH07_03455"
                     /inference="COORDINATES: protein motif:HMM:NF013647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HNT34033.1"
                     /translation="MAIKPFQKLSLIVHQNALDEAMAVLQSSASVQIVGVPSPEDQQS
                     IQARFRKPSGIGEVQDRLSELEHCITLLGEYQEPLKGLDSLFTQRIAVTSRELEELVR
                     SYNVEAILTELGDAENQLRQMNSEQDDLYKTRAILEPWTSLPIGVQALHQFEHVSASF
                     CAIPRKSWNEISSNLSLPEISDYVICSESEKDVFVLFIYARTIHDSFRKWMEERELEE
                     LSLPQSSLPPKEQLDRISQEVQEIDVRIKELNERIRSKVPERPRLMVYRDHLASVQAK
                     KMAEGNLLETDRTAFLIGWLRSDKAEEVKKRLEQTVGVFHWDLQDPEPTEEPPVVLET
                     ADVVEPFQMVTSLYGLPDYREIDPTPYIAPTFALFFGICMSDVGYGLIIALVTWLAMK
                     KLPLEPVVRQAMKVLFWGGVATA"
     gene            complement(1246..1545)
                     /locus_tag="PKH07_03460"
     CDS             complement(1246..1545)
                     /locus_tag="PKH07_03460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34034.1"
                     /translation="MKEIQKAESMAREMVEQARKSADDSRKQAAKDIESKRKEAVEKA
                     RLEVKELIAQARTQAESERDQLLKQTDSQCQTIQQQAQSRFDASVDLALRRLSER"
     gene            complement(1725..2252)
                     /locus_tag="PKH07_03465"
     CDS             complement(1725..2252)
                     /locus_tag="PKH07_03465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34035.1"
                     /translation="METMLHRGMRISVWLIPILVLIMLPVRAFAPRMEDTSGSTSEDG
                     IPEARRRAQQQYKEQATRQHQMGTAQSEEDSDVVVEYYDKGSLDRQAGPSATTKLSQR
                     EFDQSDTISIPKADQSTIDVPHPDRVFSPESAKTKQSGSSGLKKIVLGGFILLAVVAY
                     LKIRALEKAGVRKPK"
     gene            complement(2268..>2846)
                     /locus_tag="PKH07_03470"
     CDS             complement(2268..>2846)
                     /locus_tag="PKH07_03470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34036.1"
                     /translation="PPTWPKDGTPPETVAEANKNADFLGVASRGSIVLGDYTTSDWNS
                     NVGKYIKPDFTAPYIDEDGTWFHGNYTAADGVKVDGTTRRYYESSFSNAFFRAQVRTQ
                     QIRTLNGLFYTNHLFAGRMTNATINGSVIMRDEAIIYNTSFDLNYDYRAREDGEEYID
                     IDLPKAARMAQIAWLYGTFEHNEGDLEAMFGE"
     assembly_gap    2847..2856
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2857..3872)
                     /locus_tag="PKH07_03475"
     CDS             complement(<2857..3872)
                     /locus_tag="PKH07_03475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly PilX N-terminal domain-containing
                     protein"
                     /protein_id="HNT34037.1"
                     /translation="MARTSRDSKGIVLPVVLILALLAAIYVAAVLNVSRTDLQQVRSQ
                     QEMTEAMYLAEAGLNVLAEKYWIGYRESNPAHRTQWLDTHLEDVDLFEYNGSIGDSRG
                     TYTATVRRVYSTRVAGERVAIIESKGQVGSHERAVVRAVLYGLGRSRVFDYAYFANNF
                     GWMWGAGITTNGDIRSNGNFNLKNPTINGDVYASVNNEIGADGHIEGDTRQKTLDWYR
                     THYGNRVRPTTLDGYKFGYDGESEWFEGEEILEMPYLKDTSYYEQLAQSRNSTLRVGG
                     GGPTVNGVHNGNLVIYGTAANPIEINGPVVITGDVVLGGVIRGQGTIYSGRNVHIVDD
                     ITYET"
     gene            complement(3929..4396)
                     /locus_tag="PKH07_03480"
     CDS             complement(3929..4396)
                     /locus_tag="PKH07_03480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34038.1"
                     /translation="MSIMLLVAGMSVSFFGLIQWNTASVAGEIFNRQDAQNCINQLGR
                     YVYPATRLENSEDGTNMVLVWRDDNAPPTNLDITDDTTGVIYFDSAQKALFYMPDREF
                     QGNYEIVAQGIENCQMSVAGNVLRAQITFAAGPKRSPKTKTLNVSLAARNIPR"
BASE COUNT          969 a         1220 c         1099 g         1188 t
ORIGIN      
        1 gctgttgcca caccgcccca gaacagaacc ttcatcgcct gcctgacaac gggctccaat
       61 gggagttttt tcattgccag ccatgtgaca agggcgatga tgaggccgta gccgacgtct
      121 gacatacaga ttccgaagaa cagagcaaaa gttggagcaa tgtagggggt tgggtcaatt
      181 tcacgatagt cgggcaaccc gtagaggctt gtcaccatct ggaaaggttc gaccacatcg
      241 gctgtctcta gaacaacggg aggctcctct gtaggttcag gatcctgcaa atcccagtga
      301 aagacgccaa cagtctgttc aaggcgtttc ttgacctctt ctgctttatc tgaccgcaac
      361 cagccgatca gaaatgcagt tcggtctgtt tcgaggagat tcccttcagc catcttcttg
      421 gcctgcacag atgcgagatg atcccgatac accatcaatc gtggacgctc aggaaccttg
      481 cttcggatgc gttcattcaa ctctttgatc cggacatcga tctcctgaac ctcttgcgag
      541 atccggtcca actgttcctt gggaggcagt gaactttggg ggagactgag ttcctccaac
      601 tcacgttcct ccatccattt tcggaaagaa tcgtgaatag tccttgcata gatgaaaagg
      661 acaaacacgt ccttttccga ttcggaacaa atcacatagt ctgagatctc cgggagcgac
      721 aggttggatg agatctcgtt ccacgatttc ctgggaatcg cgcagaagga cgctgagaca
      781 tgttcaaact gatggagagc ttgaacacct atcggaagac tcgtccacgg ttccagaatt
      841 gctcttgtct tgtagaggtc gtcctgttcc gagttcatct gcctcaattg gttctctgcg
      901 tcgcccaact cggtgagaat cgcttcgacg ttgtaacttc tgacgagttc ttcaagttca
      961 cggcttgtca ccgcaattct ctgcgtgaag agtgaatcca agcctttgag cggttcctga
     1021 tattcaccga gaagggtaat gcagtgctct agttctgaaa gtctgtcctg cacctcgcca
     1081 attcccgaag gtttccgaaa gcgtgcttga atgctttgct ggtcttcagg gctcggaaca
     1141 ccgactattt gaacggacgc actggactgc agcacagcca tcgcctcgtc caaagcgttc
     1201 tggtggacga tcaacgagag tttttggaaa ggcttaatcg ccattctacc tctccgaaag
     1261 gcgtcgaagc gccaagtcaa cggatgcgtc aaagcgactc tgcgcctgct gctggattgt
     1321 ctgacattgg gaatcggttt gcttgagtag ctgatcccgc tctgactcgg cttgtgtgcg
     1381 ggcttgtgcg atcagctcct tgacttccaa gcgagctttc tccactgctt cttttcgttt
     1441 ggattctatg tcctttgcgg cctgcttgcg tgaatcatca gcggacttgc gcgcctgttc
     1501 gaccatttcc cgtgccatgc tctccgcttt ctgtatctct ttcaaagata aaacatccaa
     1561 gggagtcacc tctatcgggt agagtctcta gtgctttttc ccattcacgt tgccaaagac
     1621 atacgaatca tcacatttcg agcaggattt gtcaagagat aagagtcgtt gttattcgaa
     1681 gagaacgcct gcaataggcc tcttttgact gtggcaggga tgcgctactt cggtttccga
     1741 actcccgctt tctccagtgc tctaatcttc agatatgcca ccacggcgag gagtatgaaa
     1801 cctcccagca ctatcttctt gagtccagag gaacccgact gttttgtttt tgccgactct
     1861 gggctgaaca ctcgatccgg atgaggaaca tcaattgtgc tttggtcagc ctttggaatt
     1921 gatatcgtgt ctgactggtc gaactctcgc tgggagagtt tggtggtggc ggacggtccc
     1981 gcctgacgat ccaggctccc cttgtcgtag tattccacca ctacatctga gtcttcctcg
     2041 gattgcgcgg tgcccatctg gtgctggcgg gtcgcctgtt ccttgtactg ttgttgggct
     2101 ctgcgtctgg cttcagggat tccgtcttct gaggtcgatc ctgacgtgtc ttccattcgc
     2161 ggagcaaacg ctctgactgg aagcattatc agaacaagga tagggatcag ccaaaccgaa
     2221 atacgcatcc cacggtgcaa cattgtttcc attctcccgt cttttgatca ttctccaaac
     2281 atggcttcca agtcgccttc attgtgctca aaggtgccat agagccacgc gatctgcgcc
     2341 attcgagctg ccttgggtag atcaatgtct atgtattctt cgccatcctc gcgggcacga
     2401 tagtcatagt tcaagtcaaa ggaagtattg tagatgatcg cttcatctct catgatcacc
     2461 gatccgttga tcgtggcatt ggtcatccgg cctgcaaaga ggtgattggt gtagaacagg
     2521 ccattaagtg tcctgatctg ttgcgtacga acctgcgccc tgaagaaggc attgctgaaa
     2581 gacgactcat agtaacgtcg ggtcgtcccg tccaccttga ctccgtctgc agctgtatag
     2641 tttccatgga accaggttcc atcttcatca atatagggag cggtgaaatc aggtttgatg
     2701 tacttgccca cattgctgtt ccagtcggat gtagtgtagt cgccaaggac gatgctgcct
     2761 cgcgatgcca ctccaagaaa atcggcgttc ttgttggctt ctgccaccgt ttcgggtggg
     2821 gttccatcct ttggccaggt ggggggnnnn nnnnnngttt cgtaagtgat gtcatcaaca
     2881 atatgcacat ttcttccaga gtagattgtt ccctggcctc gaatgacccc tccaaggaca
     2941 acgtcgccag tgatgaccac tggcccattg atctcgattg gatttgctgc cgtaccgtag
     3001 ataacaagat tgccattgtg aaccccattg actgttggcc ctcccccacc aacgcggagt
     3061 gttgagttgc gactctgggc gagttgctcg taataggaag tgtccttcag atagggcatt
     3121 tcgaggattt cctctccctc aaaccactca ctctcgccat catatccgaa cttgtagccg
     3181 tccagggttg tcggtctcac tcgatttccg tagtgagttc gataccagtc cagagtcttt
     3241 tgcctcgtgt ctccctcgat gtggccatct gcgccaattt cgttgttgac actggcatag
     3301 acgtcgccgt tgatcgttgg attcttgagg ttgaaattgc cattggagcg aatgtcgcca
     3361 ttggttgtga ttccggctcc ccacatccag ccaaaattat tcgcgaaata cgcatagtca
     3421 aagactcggg agcgtcccaa gccatagaga accgcccgaa cgacggcacg ttcatgggat
     3481 cccacctgtc ccttactctc gataatcgcc actcgctctc cggcaaccct tgtggagtag
     3541 actcgacgaa ccgtggccgt gtatgttccg cgtgaatcgc cgatactgcc gttgtactca
     3601 aagagatcaa catcctccaa atgcgtgtcg agccattgag tcctgtgcgc cggattggat
     3661 tcccgatatc cgatccagta tttctccgcc agaacattca gaccagcctc ggcaaggtac
     3721 atggcctctg tcatctcctg ctgcgatcgc acctgctgca agtccgtcct ggagacattc
     3781 aataccgctg caacgtaaat cgcagccaac agtgccagga tcagcacaac gggcagtacg
     3841 atgccttttg agtctctgct tgttcgtgcc atagccatca ctccccttca agaatcgaaa
     3901 agcccttgtt cattcccgcg ccatcctttc aacgtggaat gtttcgggcg gccagagaaa
     3961 cattcagcgt ctttgtcttg ggcgaccgct tagggccggc cgcaaaagtg atctgtgccc
     4021 gcaggacgtt ccccgcaaca ctcatctgac aattctcaat cccttgggcg acaatctcat
     4081 aatttccttg aaactcacga tccggcatgt agaagagcgc cttctgtgcc gagtcgaaat
     4141 agatgacccc ggtcgtgtca tctgttatgt ccaggttagt gggaggagcg ttgtcatccc
     4201 gccagaccaa gaccatgttc gtgccatcct cgctgttttc caatcgcgtt gcagggtaca
     4261 cataacgccc caattggttg atacaattct gagcgtcttg gcggttaaaa atctctcctg
     4321 ccacgctcgc ggtattccat tgaatcaggc cgaaaaaact gaccgacatg cctgccacca
     4381 gaagcatgat ggacatacca atgagaatct ctatgagtgt gaaaccactt taggctttcc
     4441 ttctgtattc tggtgctttc tccatagagg acatgatcgg tcacct
//
LOCUS       DAOQUC010000133         4481 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_21631_length_4481_cov_3.453909, whole genome shotgun sequence.
ACCESSION   DAOQUC010000133 DAOQUC010000000
VERSION     DAOQUC010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4481
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21631_length_4481_cov_3.453909"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1883
                     /locus_tag="PKH07_03485"
     CDS             <1..1883
                     /locus_tag="PKH07_03485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34039.1"
                     /translation="AVPKVDNPPSRYRRELSKMVFLDVWSSHPACEVEHFLERLSLLA
                     GDHVSFYTVFQNWQTGGFDALLPDSIRMPDYPPNAAVGSVEDLRGLCKVGRQQGRFAF
                     RTNYQLFRDNSPSAKDGTAAAALDESGKPKWHTRSEDALAVARRQESEIRRLFSPNAP
                     FTDQLTSGGSPKSYLDFNPAVPEGNTMRWVASKQRELAEYLHEAIGGPLGSETLNAEY
                     LLGKWVATGDFGITNGHSRLVCPDYKLRRLHHLSTFHGMGLFYRFFEPPEFHNFHSGR
                     RAWQSDQELQDSYRCSEVLFGNAGYIYALDLPWSYVLTECFVIGRLQRYYALQRVRDV
                     YYQDGEEWKTLEQLVAEGNSVNFNFWEEQTPLLARVRVEYENGLTVVVNRAELPLMVN
                     IGNTLMKLPQNGWMAWGDGILAYSACFPGTQHRVDFLSEQESGLEYLDPRGEVHRATS
                     LITLWRHGKVIFQVDADRGIAYIKGKRTDLNYPQPASLSVLNFNFAEGTKGWRSEFGV
                     LKTEETPEGLRLNCIADGPFLMSPPLEIDADSIMKITIRLRSASGTFGKLYFLTKDDP
                     RLTEEQVFSFQLSTTDEWQTIEIEPHKHPKWSGQTITQLRLDPAKGVTNSVVTLGSII
                     GQ"
     gene            complement(1915..3699)
                     /locus_tag="PKH07_03490"
     CDS             complement(1915..3699)
                     /locus_tag="PKH07_03490"
                     /inference="COORDINATES: protein motif:HMM:NF019193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylglucosaminidase domain-containing
                     protein"
                     /protein_id="HNT34040.1"
                     /translation="MRNPFLVLCCLGLILGVVGSNVQAQESNRSIVRLKGSDFEGGGA
                     GAFGNIKYDAWGVNYVYARATGRHAQMQAEFTLTQIPRSGLFLHLRAMNQDTGGACPI
                     KIEINDTLVHKGPTDLPNRVWAWQMFPIADGVLQEGVNRIVISNELQDGTLGMPPWFM
                     LHRCIIGAEHLSKLSQPDISEDFHIVLPKEKKPFPEPYPEGEKESGFAFRGTKGWLWK
                     AEQYLEEIPTLVQCKMNFLMVCYGSMVDIEHYGWGHPEANRWWEPLPLKKKQAYAQVI
                     KECQKHGIEFCFGMNPNLGSKRILDYSSPKDYDDLWQHYQWAQDLGVTWFNISLDDIT
                     HGIDASGQAKLVNDIFRRLRSDDPKAQMIFTPTHYWGTGGDDPYLAALAEEIHPDVYL
                     FWTGPQVVSPSIRRAEAQAYRNAVRHRLFIWDNYPVNDGAPTLHLGPVLGRDADLPEV
                     VDGYMSNPLHSQNQINRLPLMTCADYAYNPRQYDPMRSITQAILHLGKNRRQQETLRD
                     LVEYYPGMLVLNHPNTAWNPVRQQFLEIMEKPHSYRLAFAYIQSFEAMVHQMNRVFPR
                     QFRATRKTLHDDLQLLKSGFEAKYTMME"
     gene            complement(3769..>4481)
                     /locus_tag="PKH07_03495"
     CDS             complement(3769..>4481)
                     /locus_tag="PKH07_03495"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNT34041.1"
                     /translation="SYLKDITIYPYTEGLENALKGLGEDGIPHTWLGRTPFQQMWIEW
                     VRMEDFSVHLVDCEDIVQECMNLAGEVMRQMFHAVKDAPAPYFVFGDNITAPLIGERY
                     FRQYCVPYYNELADILSDRGIPVFVHMDGDLKPLWKAISESKVGGLDSYSPTPDNDTS
                     PADAITNMPNVKLGMNFPSSVHLASPEEIYRTTWDILQQAGHSGRLQIQISENPPPGA
                     WKKSYPEIVRAINDFGKP"
BASE COUNT         1038 a         1148 c         1218 g         1077 t
ORIGIN      
        1 ctgccgttcc caaagtggac aacccaccca gtcggtaccg cagagagctt tccaaaatgg
       61 tgtttctgga cgtatggagt agtcatccgg cttgtgaagt ggagcatttc ctggagcgtt
      121 tgagtcttct cgccggagac catgtctctt tctatacagt gtttcagaac tggcagactg
      181 gaggattcga cgcactcttg cccgactcga ttcggatgcc cgactatccg cccaatgcag
      241 ccgtcggcag tgtggaagac ctgagaggac tctgcaaagt cggcagacaa cagggacggt
      301 ttgcctttcg cacgaactac cagctgttcc gcgacaactc accgagtgcg aaagacggga
      361 ctgcggctgc ggcactggac gagagcggaa aacccaagtg gcacacgcga tctgaggacg
      421 ctctcgcggt tgcccggcga caggagtcag agattcgccg attgttctct ccaaatgctc
      481 cgttcacaga ccaactcacg agcggtggat ctccgaagag ttaccttgac ttcaatcctg
      541 cggttcccga agggaacacg atgcgctggg ttgcttcaaa gcagagagaa ctggctgagt
      601 atctccatga ggcgattggt ggcccgctgg gttcagagac cttgaatgcg gagtatcttc
      661 tgggcaagtg ggtggcgacc ggggattttg gtatcacaaa cggacattca cgtctcgtgt
      721 gtccagacta taagcttcgg agactgcacc atctgagcac ttttcacggc atggggctct
      781 tctatcggtt ctttgaaccg ccagagtttc ataacttcca ttctggcagg agagcatggc
      841 agagtgatca agagttgcag gacagttatc gttgttcgga agtgctcttt ggaaacgcgg
      901 ggtacatata cgctctcgat ctgccttgga gttacgttct gaccgagtgc tttgtcattg
      961 ggcggcttca gaggtactac gcgcttcaga gagttcggga tgtctactat caggatggag
     1021 aagaatggaa gacgctggag caactggtcg ctgaaggcaa ttcggtcaac ttcaatttct
     1081 gggaagaaca gactcctttg ctggcgcgtg tccgagtgga atacgagaac ggtctgaccg
     1141 tcgtggtcaa ccgagccgag ttacccttga tggtgaatat cgggaatact ctgatgaaac
     1201 ttcctcagaa tggttggatg gcatggggtg acggaattct tgcgtactcg gcctgctttc
     1261 caggaacgca gcaccgtgtg gactttctct cggaacagga gagcggactt gagtatcttg
     1321 atcccagagg tgaagtgcac cgtgcgacaa gtctcatcac cctttggcga cacggaaaag
     1381 tcatctttca ggttgatgca gatagaggta ttgcctacat caaagggaag cgaacagatc
     1441 ttaattatcc acagcctgct tctttgagtg tcttgaattt caatttcgcc gaaggaacca
     1501 agggctggcg ttccgagttt ggagtactca agaccgaaga gactcccgaa gggttgagac
     1561 tcaactgtat cgctgacgga ccgttcctga tgagtccgcc attggagatt gatgcagata
     1621 gcatcatgaa gatcacgatt cggcttcggt ctgcctcggg aaccttcgga aaactctact
     1681 ttctgacgaa agacgatccg cggttgacgg aggagcaagt gttttcgttt caattgagca
     1741 ctaccgacga gtggcagact atcgagatcg aaccgcacaa gcacccaaaa tggtcaggac
     1801 agacgatcac ccaactgcga ctggatccgg cgaaaggtgt cacaaactca gttgtgacac
     1861 tcggttccat cattggccag tgagaacctg acagggcaca gcgatccgag gtctttattc
     1921 catcatcgtg tatttggctt caaatccgct tttcaacaac tgaaggtcat cgtggagcgt
     1981 cttgcgggtc gcccgaaact gtcggggaaa gactcgattc atttgatgca ccattgcctc
     2041 gaaggactga atgtaagcga atgcgaggcg ataagagtgt ggtttttcca tgatctccag
     2101 gaactgctgc cgaaccggat tccaggcggt gtttggatgg ttgagaacca acatgccagg
     2161 atagtactcc acaagatccc tcagcgtttc ctgttgtctt cggttcttcc ccaagtgcag
     2221 gattgcctgt gttatggaac gcatcggatc gtattggcga gggttgtagg catagtcggc
     2281 gcaggtcatc aaaggcaaac ggttgatctg gttttgggaa tgcagagggt tggacatata
     2341 gccgtcaaca acctcgggca gatcggcgtc gcgtccaaga acagggccca gatgaagcgt
     2401 tggtgctcca tcgttgaccg gatagttgtc ccagatgaaa agccgatgac gcacggcgtt
     2461 gcggtaggct tgcgcttcgg cacgacgaat actcggcgac acaacctgcg ggcctgtcca
     2521 gaaaagatag acatccggat gaatctcctc agccaatgct gcaaggtatg gatcatcacc
     2581 gcccgttccc cagtaatggg ttggcgtgaa gatcatctgc gccttgggat catctgaacg
     2641 cagccggcga aaaatgtcat tgaccaactt ggcctgaccg gaagcatcta ttccgtgcgt
     2701 gatatcatcc aacgatatgt taaaccatgt gacccccaaa tcctgtgccc attggtaatg
     2761 ctgccagaga tcgtcatagt cctttgggct tgagtaatcg aggattcgct tggagccgag
     2821 gttcgggttc atcccgaaac agaattcgat gccgtgcttc tggcattctt tgatgacttg
     2881 ggcgtacgcc tgctttttct tcaggggcag cggttcccac cagcggttgg cttccggatg
     2941 accccaaccg tagtgctcga tgtccaccat acttccgtaa cagaccatca gaaagttcat
     3001 cttgcactga acaagcgtcg ggatctcctc aaggtactgt tccgccttcc agagccatcc
     3061 cttggtgcca cggaatgcaa aaccggactc tttctctcct tcaggatagg gttcgggaaa
     3121 gggtttcttt tcttttggca gcacaatatg gaagtcctcg ctgatatcag gctgcgagag
     3181 tttggacaaa tgttccgctc cgatgatgca gcggtgaagc atgaaccacg gcggcattcc
     3241 gagggtaccg tcttgaagct cattcgagat aactatcctg ttaacgcctt cttggagcac
     3301 tccatcagca attggaaaca tctgccacgc ccagacgcga ttgggaagat ctgtcggacc
     3361 tttgtgaacg agcgtgtcat ttatctctat cttgattgga caggcgccac cggtgtcctg
     3421 attcatggcg cgcaggtgga gaaatagacc gcttcgaggt atctgcgtca gagtgaactc
     3481 tgcctgcatc tgagcatgac gacccgtggc gcgagcgtag acatagttca ctccccaggc
     3541 gtcgtacttg atattgccaa aggctcccgc gccgccaccc tcgaaatcac tgcctttcag
     3601 tcggacgata ctgcgatttg attcttgagc ctgaacgttg gaaccaacaa ctccaaggat
     3661 cagtccgaga caacaaagga ctagaaaagg atttctcatg atgcaccctc catctgctgt
     3721 ttgctcagtg cgtaggacgt cactggctca ttggcttctc tgacacgctt agggctttcc
     3781 gaaatcattg atggcacgga caatttcagg ataactcttc ttccaggctc caggcggcgg
     3841 gttctctgaa atctggattt gcagcctgcc cgaatgacct gcctgttgca gtatgtccca
     3901 ggttgtcctg tagatttcct caggtgatgc cagatgaacc gatgagggaa aattcatgcc
     3961 gagtttgaca ttcggcatgt tcgtgatcgc atcggcgggg ctggtatcat tgtcaggcgt
     4021 gggtgagtag gaatcgagtc cgcccacttt cgactccgag atggccttcc acagcggctt
     4081 caagtcccca tccatgtgca caaagacggg aatgccccgg tcgctgagga tgtctgccag
     4141 ttcgttgtag tacgggacac agtattggcg aaaataccgc tcaccaatca gaggagccgt
     4201 gatgttgtcg ccgaagacaa aatacggtgc gggtgcatcc ttgacagcgt gaaacatctg
     4261 tcgcatgacc tcaccagcca ggttcatgca ctcttgaacg atgtcttcac agtccaccag
     4321 atgcacagag aaatcctcca tgcgaaccca ttcgatccac atctgctgaa agggtgttct
     4381 tcccaaccac gtgtgcggga ttccatcttc cccaagacct ttcagcgcat tctcaagacc
     4441 ttccgtgtaa ggatatatcg tgatgtcctt cagataagag c
//
LOCUS       DAOQUC010000134         4478 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_21658_length_4478_cov_3.332806, whole genome shotgun sequence.
ACCESSION   DAOQUC010000134 DAOQUC010000000
VERSION     DAOQUC010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4478
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21658_length_4478_cov_3.332806"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..341)
                     /locus_tag="PKH07_03500"
     CDS             complement(<1..341)
                     /locus_tag="PKH07_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013998617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HNT34042.1"
                     /translation="MSANQIEIRIWSPEEKLPREQLKSLQLRRLQETVSRAFHNVSHY
                     HEALERDRLTPDSIKSLDDLRRIPFTVKDDLRKQYPLGMLAVPREKVVRYHASSGTTG
                     KPTVVAYTRKD"
     gene            complement(406..717)
                     /locus_tag="PKH07_03505"
     CDS             complement(406..717)
                     /locus_tag="PKH07_03505"
                     /inference="COORDINATES: protein motif:HMM:NF014687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HNT34043.1"
                     /translation="MQEDPYSPFERHQLILRDLLAVDRTVLANERTFLAYIRTALAFF
                     LSGITILKFFESMIIRVIGIAFIPLGVVILIIGTWRFTHVQKAIGRLRKNPSQLSGTG
                     D"
     gene            1273..1698
                     /locus_tag="PKH07_03510"
     CDS             1273..1698
                     /locus_tag="PKH07_03510"
                     /inference="COORDINATES: protein motif:HMM:NF012622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I domain-containing protein"
                     /protein_id="HNT34044.1"
                     /translation="MPKKVYYSAMTMMSRVHLEKPRWRSFVSAIVFALLILGLPLRPT
                     MANPEATPAPSASPCDLNEDGFVSTIDVLLFSLTFGEKVSEALPEEKHQIAKCDFNND
                     GRVDETDLFRIISEWKPLVQTDKVASPSLLKETTPEQSW"
     gene            complement(1856..2488)
                     /locus_tag="PKH07_03515"
     CDS             complement(1856..2488)
                     /locus_tag="PKH07_03515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34045.1"
                     /translation="MEATTGMMDLFGIWIAGFLTLAIYSFLYKDNPAYKIAEHIFVGV
                     SAGYTICITWHNILRQDVFQRLAEGQWIVLFPVAMGLLMYTRFTRNHAWISRLSMAFV
                     VGAGAGIGVPNTIQALLIRHTQSTIGPFITAQSVSWNELCILFGVVSTLVYFFFSVEH
                     KRFVGVISWIGVIFLMIYFGASFGYTVMARVSLIIGRMRFLILEWLPSFF"
     gene            complement(2502..3338)
                     /locus_tag="PKH07_03520"
     CDS             complement(2502..3338)
                     /locus_tag="PKH07_03520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34046.1"
                     /translation="MTWDEKLQAIDRRIIFLLVGIAVALPIIFRLPIPIPVVTPEVMG
                     VYERIEALPSNAGVFMVFDYEPATTPELNPMAEALLRHCFQKNLRVVCATLYSSAPGI
                     MESLLKEVGDRYGKKAGIDYTLLGYRAGGYAVILRIVDNLKKGFPRDYYDNLTTEAPV
                     LEGLDRLKDFAYLMCLHDDSTIQTWVTYGYERTGIPMASGCTAVMATGSYPLLHAKQI
                     NGILGGLKGAAEYEKCLNYEGVAHQGMFSQSVVHMLIIAMIVLGNVGHFLRHRRNRQA
                     RS"
     gene            complement(3340..4011)
                     /locus_tag="PKH07_03525"
     CDS             complement(3340..4011)
                     /locus_tag="PKH07_03525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34047.1"
                     /translation="MNFVRRKLPVLIVFLMGMVMMLRFYCPRYSSDLLLDEVNKWTMI
                     IGGFTLVIGVVSLIRVHYQRVRRRQPGYGYSLVMYFFLFLTVLVGVPRNVGSLKLGIE
                     SGSPFMWIFENLNVPVSSAMFSILGFYVASAAYRAFRAKTFEAGVMLVAAVIVMFGRV
                     PLADVISEPLPEWLKITNLTSWILNVPTVAARRAIILGMGLGSIATALKIIFGLERTY
                     MGGGR"
     gene            complement(4073..>4478)
                     /locus_tag="PKH07_03530"
     CDS             complement(4073..>4478)
                     /locus_tag="PKH07_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34048.1"
                     /translation="VTDDAFGYTAAVDGLIVREKPAANFYLGTFSAEALLLAETGAMT
                     GAIQIAGTDSTYQIPFFIVACDYTLIGEELYAASAYLSREPKLLGSLRGQDCGKVILM
                     AAIVIGTVGACLYTVFPDAEWLLWLHHFFTKT"
BASE COUNT         1131 a         1216 c         1142 g          989 t
ORIGIN      
        1 aagtcctttc gggtgtaggc gaccacagtc ggcttgccag tggttcccga agaagcgtga
       61 tatcgaacca ccttctccct cggtacggcc agcattccaa gtgggtactg cttccgcagg
      121 tcatccttga cggtgaaggg aatccgcctc agatcgtcca gagacttgat ggaatcaggc
      181 gtgagtctgt cacgttccaa cgcctcatgg tagtgggaga cgttgtgaaa agcccgagag
      241 acggtctcct gaagtcgccg caactgaagc gacttcaact gctcccgggg aagtttctcc
      301 tctggactcc agattcggat ttctatctga ttggcactca tcttcgttgt cctcactcct
      361 gtttcgttgt cttcccttcc acccgcacat acgaggtgtg tgtcttcagt ccccggtgcc
      421 gctcagttga gatgggttct ttctcagtct gccaattgcc ttctgtacat gcgtgaatct
      481 ccaggttccg atgatcagga tgaccacacc cagaggaatg aaggctatgc cgatgacgcg
      541 aatgatcatg ctttcaaaaa acttcaagat cgtgatgccc gacagaaaaa aagccagtgc
      601 ggtgcgaata tatgccagga acgttcgttc attggcgagt accgttcggt caactgcgag
      661 caggtcacgc agaatcagct gatgccgctc aaatggggaa tagggatctt cttgcatgga
      721 tatcctcctg aaaagaccgc cccctcttac cctgttttgg tgagggaaat cctgaattcc
      781 cttccctcac caaggagcag cagggggagg tcaagtcaaa taacctcgaa caactgcatc
      841 cgatcagatc agatggagcc cgcatataca tgggggcact agcccgcttt ccggaaagcg
      901 gactcccagg catcttggct cagtgtggca aacaagcgca cgcaggagtg gaccacccag
      961 cctccaactg ccactgctcg tctcaccaag aaggcataat atgaattcct ggcgcgagat
     1021 tcaagcgaag tagattgtaa tctgcgctgt tggggtaatt catgaatgac ccgtactcgg
     1081 gcaggagcca ccgtgcacat gcattcggtt tccacaaatg atgcgtatct gtctcagcct
     1141 ccaatgtcaa tctggctatt ccgttgagca ggccactctt gcgaacacgt gcgcatcgga
     1201 agaagcagac ctcttcaaag tctggtttca aaagaggctt cttacacccg cattttagcg
     1261 gaagttcctg ttttgccgaa aaaggtatac tattccgcta tgactatgat gagcagagta
     1321 catctggaaa agcctcggtg gagatcgttc gttagcgcga tagtgttcgc tcttctaatt
     1381 ctgggcttgc ctctacgacc gactatggca aatcccgaag caactccggc tccttccgcc
     1441 tcgccatgcg atctcaatga agatggattt gtctccacca ttgacgtact gttgttcagt
     1501 ctgacattcg gtgaaaaggt cagcgaagca ttacccgagg aaaagcacca gattgccaag
     1561 tgtgatttca acaatgatgg cagagtggat gagacggatc ttttccggat catatccgag
     1621 tggaaaccac tggtgcagac cgataaggtt gcctcacctt cactgctgaa agagaccaca
     1681 cccgaacagt cctggtagca tgaaccagga tgtctctctt ccctttactg gtttatcgtg
     1741 atgccatagg gcatgtcctt acaagcggag cagcgcctgc agacgtgcgg acaaagccca
     1801 ctccctggaa cacatattcc aagaaggttg ggagtcagtg acactggtgg gtcaatcaga
     1861 agaagctcgg aagccattcg aggataagga aacgcattcg accgatgatc aacgacacgc
     1921 gtgccataac cgtatagccg aacgaagctc caaaatagat catcaggaaa atgaccccta
     1981 tccaggagat gacaccaacg aaacgcttgt gctccacgga gaagaagaaa tagaccagag
     2041 tgctcacgac tccgaacagg atgcacagct cattccaaga gacgctctgg gctgtgatga
     2101 agggtcctat cgtgctctgt gtgtggcgga tcaagagggc ctgaatcgtg ttgggaactc
     2161 cgatgcccgc tcccgcgccg accacaaagg ccatggacag tcggctgatc caggcatgat
     2221 ttcgagtgaa ccgggtgtac atcaaaagtc ccatggcaac ggggaaaagc acaatccatt
     2281 gcccttccgc cagccgttga aagacatcct gacgcaggat attgtgccat gtgatacaga
     2341 tggtgtatcc cgcagaaaca ccaacaaaaa tatgctcggc gatcttgtac gccggattgt
     2401 ctttatagag gaaactgtag atcgccaagg tgagaaaccc tgcaatccaa attccgaaaa
     2461 gatccatcat gcctgtggtt gcttccatat ctctatcctg gtcaactcct tgcttgcctg
     2521 ttgcggcgat gtcgaaggaa atgaccgacg ttgcccagca cgatcatggc gatgatgagc
     2581 atgtgtacca ccgactgaga gaacattccc tgatgggcaa cgccctcata gttgaggcat
     2641 ttctcgtatt ccgccgctcc cttcaagccg cctaggatgc cgttgatctg tttggcgtga
     2701 agcagcggat acgatccggt tgccatgact gcggtgcaac ccgaggccat tggaataccc
     2761 gttcgctcat atccataagt gacccaggtt tggatcgtgg agtcatcgtg caggcacatc
     2821 agataggcaa agtccttcaa ccgatccagg ccttccagga cgggagcctc ggttgtaagg
     2881 ttgtcgtagt aatctcttgg aaatcccttc ttcaggttgt ccacaattcg gagaatcact
     2941 gcgtatccac ctgcacggta tcccaaaagt gtgtagtcaa tgcccgcttt cttgccatag
     3001 cggtcgccca cttccttcag cagcgattcc atgatccctg gagcgctgga gtacagtgtg
     3061 gcacacacca ctctcagatt cttctggaag cagtgtcgaa gcagcgcctc ggccatcgga
     3121 ttgagttcgg gtgttgtggc aggctcatag tcgaagacca taaacactcc ggcattggag
     3181 ggaagggctt ctattctctc atagactccc atgacttcgg gcgtgacaac ggggataggg
     3241 atgggcagcc tgaagatgat gggtagagcc acggcgatcc caaccaggag gaagatgatg
     3301 cgccggtcta ttgcctgaag tttttcatcc caggtcatgc tatcttcctc cccccatgta
     3361 ggtgcgctca agaccgaaga tgattttcag cgccgtcgca atggagccaa gacccatacc
     3421 aaggatgatt gcgcgtcgtg ccgctacggt cggcacgttg agaatccaac tggtcaggtt
     3481 ggtgatcttg agccactcgg gaagcggttc ggaaatcacg tcggccaaag gaacccgacc
     3541 gaacatgacg attacggcag caaccaacat gacccccgct tcgaaggttt tggctcgaaa
     3601 agcccgatac gcagcggacg caacatagaa acccagaatt gagaacatgg cgctggacac
     3661 gggcacgttc aggttttcaa aaatccacat gaacggggat cccgactcaa tgcccagttt
     3721 cagtgaacca acatttctgg ggactccaac caggacggtg agaaaaagga agaagtacat
     3781 caccaggctg taaccatagc cgggctgccg tcgccgcact ctctgatagt gaacccgaat
     3841 caggctcaca accccaatga ccagggtgaa accaccaatg atcatcgtcc acttgttgac
     3901 ttcatcaaga agaaggtcgg aactgtacct cggacaataa aaccgaagca tcatgaccat
     3961 ccccatcaag aagacgataa gtacgggcaa cttgcgtctg acaaaattca cgttcttagt
     4021 ctctatctcc tcagagagcc gagatgggct ctccgctcca aatcagatac tcctacgtct
     4081 ttgtgaaaaa gtgatgcaac cacaaaagcc attccgcgtc tgggaagacc gtgtaaagac
     4141 aggcaccgac tgtcccaatt acaatcgccg ccatcagaat gaccttccca cagtcctggc
     4201 ctcgaagact gcccaagagc ttgggctcgc gcgacagata ggcacttgcc gcgtagagtt
     4261 cctctccaat gagcgtgtaa tcacacgcaa cgatgaaaaa aggaatttga tacgttgagt
     4321 ccgttcctgc gatctgaatc gcgccggtca tggcgccagt ttccgccagc agcagcgctt
     4381 cggcggaaaa cgtgcccaag tagaaatttg ctgccggttt ctcgcggaca atcagaccat
     4441 ccaccgcagc ggtgtatcca aaagcgtcgt ccgtaacg
//
LOCUS       DAOQUC010000135         4447 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_21877_length_4447_cov_2.689891, whole genome shotgun sequence.
ACCESSION   DAOQUC010000135 DAOQUC010000000
VERSION     DAOQUC010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4447
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21877_length_4447_cov_2.689891"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1873
                     /locus_tag="PKH07_03535"
     CDS             <1..1873
                     /locus_tag="PKH07_03535"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HNT34049.1"
                     /translation="VDAGEFDTFLNSYYSQSADTPSMSWKTNPTTGIIIGQVKNGSNP
                     VTDAWVTRTGSSYVTLSAADGQYTILNVPPGTYTVSASKSGVGDGSVSGVSVTAGGVT
                     RIDIALGVGPTSTPTQTPTATPTGQTPADIIIDNSNAGFSILAGSWSTGTSATDKYGA
                     DYRYNTCGTGADQVRWQGSIVTAGNYSVYCWYPQGTNRATDAPYTVYHAGGSQTFDVN
                     QQAGGGQWNLLGTFAFNAGTCTIVLTDDGIDTSKVVMADAVRWAWSGPSGPTDTPTTA
                     ATNTPTRTPTSTPTNTPTVLTNTPTNTPTNTPTFTRTNTPTNTPTNTPSNTPSNTPTN
                     TPTSAGTPADIIVDNSDTGYSSVGVDSSWLTASSATDKYGADYQYNTCGAGNDTATWQ
                     TSIPASGNYEVFVWYSAGSNRPKNAPYTVNYAGGSRMMRVNQEVNGGMWNSIGVFNFS
                     TGSTSVVMTDAADSGEAIIADAVKWSWSTATILIDDNFTNAYTGWSTDWGTSTSYYVS
                     SARAAMCNSTQNDLISGDLNTTGRSSITISFHYRDVGIDDGDDIFLQLYNGSSYANKF
                     EVGITSPESTWHYYTTTINNSGGDAAYFRSNFRVKFEGSSIDSGEYLYVDDVRVTAK"
     gene            complement(1970..2638)
                     /locus_tag="PKH07_03540"
     CDS             complement(1970..2638)
                     /locus_tag="PKH07_03540"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2817 domain-containing protein"
                     /protein_id="HNT34050.1"
                     /translation="MAPKIARKKNPTPDFPVHKSVQGRAIKTLSFGKRKKTVLLMAGV
                     HGDEKSGVLMIRRLLRKIRAAAPDALKYRIVVMPVVNPDAFSAGTRRNANDIDINRNF
                     PTRDFHANKKRSGGTEPASEPETRAVLDVVERAKPWLIISLHAALACVNYNGDAALKV
                     AKRMSSICKLEVKGDIGYPCPGSMGTYYGWERQLPVITLELPRDGADMKPTECAILDI
                     LSLE"
     gene            2810..4084
                     /gene="glyA"
                     /locus_tag="PKH07_03545"
     CDS             2810..4084
                     /gene="glyA"
                     /locus_tag="PKH07_03545"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014144470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HNT34051.1"
                     /translation="MASQQVAKQDHPFLEKFDPEIAELVFKEQERQETSIRLIPSENY
                     VSRAVMEATGSVLTNKYSEGYPQKRYYEGQQFIDAVEEIAIARAKSVFQAQHANVQPY
                     SGSPANMAAYLAFAKPGDTVMGMSLPHGGHLTHGWGVSFSGQFFKSVQYEVRREDGLL
                     DYDAIEKLAKECKPALIFAGATAYPRQIDFVRFAEIAHSVGAKLVTDIAHIAGLVVAG
                     AHPSPVPHADAVTTTTHKTLRGPRGGMILCKEEHAKAIDRAVFPALQGGPHNHTTAAV
                     AVALKEASTESFRQYGQQIVRNAKAFAEELLSQGFDLVTGGTDNHLLLIDLTYRGIPG
                     KVMAKALDKAGIVANYNTVPFDQRKPFDPSGVRMGTPAVTTRGMKEPEMVQIARWIEQ
                     IARDPEDEKTRNRVREEVRSLCTQFPVPACFV"
     gene            complement(4094..>4447)
                     /locus_tag="PKH07_03550"
     CDS             complement(4094..>4447)
                     /locus_tag="PKH07_03550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34052.1"
                     /translation="LPLAAWFVMLCLGLYFASREASLAFPVWNRFFPMFFALMLPIAY
                     LATKSLDGCAGTAFLIALSVFLLIVLALLPPLGYWFLAPVAVILQGTALIFFYKLMAS
                     SRKMYVPKTTHRLEF"
BASE COUNT         1087 a         1187 c         1210 g          963 t
ORIGIN      
        1 tgttgatgct ggggagttcg ataccttcct gaacagctat tattcccagt cggcagacac
       61 gcccagcatg tcgtggaaga ccaacccaac cacaggaatt atcattggtc aggtcaagaa
      121 cggctcgaat ccagtgactg atgcctgggt gactcgcacc ggctcgagct acgtaacgct
      181 ctctgcggcg gatgggcagt acaccatttt gaacgttccg ccaggaactt acacggtgag
      241 tgcttcaaag agtggcgtag gtgacggttc tgtgtcgggc gtctctgtca cggcaggtgg
      301 tgtcacccga atagatatcg cattgggtgt cggccctacc agcactccaa cgcaaactcc
      361 gacagcaacg ccaaccggac agactccggc ggacatcatc atagacaaca gcaacgccgg
      421 attcagcatt ctggcaggct catggtcaac gggaaccagt gcgacggaca agtacggggc
      481 ggactatcgc tacaatactt gcggcacggg tgctgaccag gttcgttggc agggaagcat
      541 tgtcacggcc ggcaactaca gtgtctactg ctggtatcct cagggaacca accgcgccac
      601 tgacgccccc tacacagttt atcatgccgg tggttcgcag accttcgacg tgaaccagca
      661 ggccggtggc ggccagtgga atctgctcgg tacttttgcg tttaatgcgg gtacctgcac
      721 gatcgtcctg acggatgatg gtattgacac ctccaaggtg gtcatggcag atgctgttcg
      781 ctgggcatgg agcggtcctt caggtccaac ggatactccg acaactgcgg caaccaacac
      841 gccaactcgc acaccgacaa gcactcccac caatactcct actgtgttga cgaatacccc
      901 gacgaatact ccgaccaaca caccgacgtt tacgcgaacc aatacaccca ccaatacacc
      961 aacaaacaca ccctccaaca cgccaagcaa tacacctacc aacactccga cgtctgctgg
     1021 aactccagcg gatatcatcg ttgataactc agatacgggt tactccagtg tgggtgtgga
     1081 cagcagctgg ctgaccgctt catcagcgac agacaagtac ggtgccgact accagtacaa
     1141 cacctgtggt gctggcaatg acacggcaac ctggcagact tccattccag catctgggaa
     1201 ctatgaagtc tttgtctggt attccgctgg atcgaatcgt ccaaagaatg ccccgtacac
     1261 agtgaactac gcgggcggct ccaggatgat gcgagtcaat caggaagtca acggcggaat
     1321 gtggaattcg ataggtgtgt tcaacttctc gactggatca acttctgttg tcatgactga
     1381 tgcggcggat tcgggagaag ccattatcgc cgatgcagtg aagtggtcgt ggtctacggc
     1441 aacgatcctc attgatgaca acttcaccaa cgcctatacc ggatggagca ccgactgggg
     1501 aaccagcact tcttactatg tttcctcggc acgcgctgcg atgtgcaaca gtacacagaa
     1561 tgacttgatt tccggcgacc tgaacacgac aggcaggtcc agcatcacca tttcgttcca
     1621 ttacagggac gtcggcattg acgatggtga cgacatcttt ctgcagctgt acaacggctc
     1681 cagctatgcc aacaagttcg aagttgggat caccagtccg gagtccacgt ggcattacta
     1741 tacaaccacc atcaacaact ccggtgggga tgcggcatac ttccgctcca atttccgagt
     1801 caaattcgaa ggcagttcga ttgactccgg tgagtacttg tacgttgacg acgtcagagt
     1861 gaccgcaaag tagcattgac ggcagtcaat aaggaatgac gcagaacggg gtagagccgt
     1921 acaggttctg ccccgtttgt atcttgagcg cttgaaaggg tttcgatttc tactccaagc
     1981 tgaggatgtc aagaatcgcg cattccgtgg gtttcatatc agcgccgtca cgagggagtt
     2041 caagtgtgat cacgggtaac tgcctctccc aaccatagta ggttcccatc gatcctggac
     2101 agggatagcc gatgtcgccc ttgacttcca gtttgcagat tgacgacatc cgttttgcca
     2161 ctttgagtgc tgcatcaccg ttgtaattca cacaagccaa cgcggcgtgc agcgatatga
     2221 tgagccacgg ctttgcgcgc tcaaccacgt cgagaactgc ccgtgtctcc ggttcagaag
     2281 caggctctgt gccccccgaa cgcttcttgt ttgcatgaaa atcacgggtg gggaagtttc
     2341 ggttaatgtc aatgtcattg gcgttgcgtc ttgtgccggc gctgaaggcg tcagggttga
     2401 ccacgggcat gaccacgatc cgatacttca gagcatctgg tgcggccgcc ctgatcttcc
     2461 tgagcagacg gcggatcatg agcacgccgc tcttctcatc accgtgaacg cccgccatga
     2521 gcagaacggt tttcttgcgc tttccaaacg agagcgtttt gattgcgcga ccttgaacag
     2581 acttgtgaac ggggaaatcc ggtgtgggat tctttttcct ggcgattttg ggtgccatag
     2641 ggttgttcca tcctcgatgt caaatgtcgg ttctatacag gacagcgcgg ggaaaggtca
     2701 acccgaaaac ctcttttgcc ttcctccttg acagcacacg gtgaacttgg gattgtatcg
     2761 gtcaagaaga tattccgttc cccgtcggga taagtcaagg agaaatctgg tggcttcaca
     2821 acaagtggca aagcaggatc atccattcct agaaaagttc gatccggaga ttgcggaact
     2881 cgttttcaaa gagcaggaac ggcaggagac gagtatccgt ctgattccat ctgagaacta
     2941 tgtgtccaga gcagtcatgg aagcaacggg ttccgtgttg accaacaagt attcggaagg
     3001 ctatccgcaa aagcgctact acgagggaca gcagttcatt gatgcggtgg aagaaattgc
     3061 cattgcccgt gccaagtcag tcttccaggc acagcacgcg aatgttcaac cttattcggg
     3121 atctcccgca aatatggcag cgtatcttgc ttttgccaaa ccgggagata cggtcatggg
     3181 aatgagtctt cctcacgggg ggcatctgac acacggctgg ggtgtcagtt tctcaggcca
     3241 attcttcaag tctgttcagt atgaagttcg ccgtgaagat gggctgctgg actatgatgc
     3301 catcgagaaa ctggcaaaag agtgtaagcc cgcactgatc tttgcaggtg cgacagcgta
     3361 tccacgccag attgactttg tgcgatttgc cgagatcgcc cacagcgttg gcgctaaact
     3421 cgtgactgac atcgcccata ttgcaggctt ggtggtggcg ggtgctcatc cgtctccggt
     3481 tccccatgcc gatgcggtga cgacgacaac gcacaagaca ctccgcggac cccgtggcgg
     3541 gatgatcctg tgtaaggaag aacatgcaaa agccattgac cgagccgtct ttccggctct
     3601 tcagggtggc cctcacaacc acacgaccgc ggcggtcgcc gtggcgctca aggaggcctc
     3661 gacggagtct ttcagacaat acgggcagca aattgttcgc aatgccaagg cctttgccga
     3721 agagttgctg agtcagggct ttgatctggt cacagggggc acggacaatc atctgttgct
     3781 gattgacctg acctatcgtg gcattccagg caaggttatg gcaaaggctt tggacaaggc
     3841 gggcattgtt gccaattaca acacggtgcc attcgatcag agaaagcctt tcgatcccag
     3901 cggtgttcga atggggacgc cggcggtgac aacgcgaggc atgaaggaac cggagatggt
     3961 tcagatcgcg cgttggattg aacagatcgc acgcgatccc gaggacgaaa agacgagaaa
     4021 tcgagttcgt gaagaagtgc gttctctctg tacccagttt ccagtacccg cgtgctttgt
     4081 ctaaagattt ggtttagaac tcaagcctgt gcgttgtttt ggggacatac atctttctgc
     4141 tcgaagccat caatttgtag aagaatatca gagcagtgcc ttgcaggatt actgcgactg
     4201 gagcaagaaa ccaataaccc agtggtggca acaacgcaag cacgatgaga aggaacacgg
     4261 acagagcaat gagaaaagcc gttcccgcac aaccatccaa cgacttggtc gcgagatagg
     4321 caatgggcag catgagcgca aagaacatgg ggaaaaagcg attccacact ggaaaggcga
     4381 gcgaggcttc tcgagaagcg aaatacaatc ccaggcacag catcacaaac caagcagcaa
     4441 gcggcag
//
LOCUS       DAOQUC010000136         4439 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_21928_length_4439_cov_2.850137, whole genome shotgun sequence.
ACCESSION   DAOQUC010000136 DAOQUC010000000
VERSION     DAOQUC010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4439
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21928_length_4439_cov_2.850137"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1044)
                     /locus_tag="PKH07_03555"
     CDS             complement(<1..1044)
                     /locus_tag="PKH07_03555"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNT34053.1"
                     /translation="MNRRDFLNALAAGTFAGCASTKASKTRATRKTSSRPNIVFLLND
                     HQLHYRHGWDSGPEIQRPNFRRLASQGADFSRAYTTCPLCAPARRTMLTGLMPHQHGQ
                     TTNAFAYPFDRETYLEHLRDSGYRSFYYGKWHAGPGTAHDFGCEGFSYPSYNNPYNKP
                     EYAEYLRRRGLPEPLMIVEQTYNSAGVRPGERYPLDRKACLGAVTGVLETPDDTHEAF
                     FLANLAVEKLQELATHPSDQPFSLRVDFWGPHQPHFPTRRFIEKYDTSQIPKYPSFDS
                     DLSDKPEVYRQEGQRPTTDENGMLIYPNPIPWEVWQKGMGYIYAHCSLVDAAGGLILD
                     ALDALGLSDNTIVI"
     gene            1206..2552
                     /locus_tag="PKH07_03560"
     CDS             1206..2552
                     /locus_tag="PKH07_03560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34054.1"
                     /translation="MRKMMLAFISAGFLCSIGVAQDVNLDGVIDSADLFALSSTWHIF
                     LKQGSTLDLMEDWQTEVTYQPARETVVLELTPEGNEPQICLDGRGVANVVHVKRIPQL
                     APDNSTVMTPQLVCSSILASGDYWQREHVLTERKPNIYLWGSYYAPLLADIVLAAPRD
                     RAGDFYAVWPHYSAGFSMVRFNRYALPQTKEYQTSTAGLGSIKRVDAAVDDDGNIHIA
                     WTTPLVLNATIEIRYSKFTPAGKMLVAPPLSLGTRLTSSGFSELFAPVIRMLGGKNPL
                     VLIDQGESAPTLAVRVDGTNGTILETKVLPQNFVEPDFVEMPNGDIALVGMIFVLNKG
                     RDIFYTVLKPDLTTRIAPVNLSNTTQQSDGASIAVVEDDFYVAWNENKDNYMRLVRLN
                     ANGSRQGNVITLSGPGQGFPFPLRLASDALGMAHIVYADGKGSSPAKVSYEKRKIR"
     gene            2728..4134
                     /locus_tag="PKH07_03565"
     CDS             2728..4134
                     /locus_tag="PKH07_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005427666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34055.1"
                     /translation="MASKKTTFALFFGNRGFFPASLQASARKETTERLERLGHQVIAL
                     DPKATRHGAVETPEEGEKYARFLQENRGKFGGVILCLPNFGDETGAVAALRDANVPIL
                     IQAYPDTFDKMDPEHRRDAFCGKFSIMDVFVQYGVPFTALKPHTVNPLGDKFQEQVAY
                     FDRLCRVVQGMRRMIVGAIGARTTAFKTVRIDEITLQEFGITVETLDLSDVFAQMKGI
                     KDTNPKYKKKAKVLQDYSNWKGVPAKAFNTIVQLGVVLDNIIEEYKMDALALRCWVEM
                     QRQLGVSPCVLLSELNDRGIPAACEVDIGNAVTMYGLSLASGKAATCLDWNNNYGDED
                     DRCILFHCGPVPNSMMTAKGDIEDHSILANAVGKGCGFGCNVGRIARVPFTYGSLLTR
                     DGNILCYLGEGAFTEDPIPEEFFGCAGVADIPNLQDALQKIGYAGYRHHVSVTPGHVA
                     APTYEAFTRYLGYCVTKV"
     gene            4187..>4439
                     /locus_tag="PKH07_03570"
     CDS             4187..>4439
                     /locus_tag="PKH07_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007618715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HNT34056.1"
                     /translation="MVYIVMGVSGCGKTTVGRVLAQRLSLPFFDADSFHSPECITKMK
                     AGIPLSDEDRIPWLTTLAKQIEVWQRSGGSVLACSALKET"
BASE COUNT         1075 a         1112 c         1192 g         1060 t
ORIGIN      
        1 gattacgatt gtgttgtcgc taagccccaa agcgtcaagg gcatcaagaa tcagcccacc
       61 agcggcgtcc accagtgaac aatgagcgta gatgtaaccc atccccttct gccacacctc
      121 ccaagggatg ggattcggat atatcagcat tccattctca tcagttgttg gacgctgtcc
      181 ctcttgacgg tagacctcgg gcttgtcaga gaggtcgcta tcgaagctgg ggtatttcgg
      241 gatctgcgaa gtatcgtatt tctcgatgaa gcgtctggtt ggaaaatgag gttgatgcgg
      301 accccagaaa tcaacacgaa gagaaaacgg ttgatcgctt ggatgtgtgg ccagttcctg
      361 aagcttttcg acagcaagat tggcgagaaa gaaagcctca tgcgtatcat ccggcgtctc
      421 cagaactcca gtcacagcac caaggcacgc tttcctgtcg aggggatagc gctcgcccgg
      481 gcgcacacca gcgctattgt aggtttgctc gacaatcatc aatggttcag ggagtccacg
      541 ccttcgcaag tactctgcgt attcgggctt gttgtacggg ttgttatagc tgggatagct
      601 gaaaccctcg caaccgaagt catgcgctgt gcccggtcca gcatgccact tgccatagta
      661 gaagctccta tagcccgaat ccctcagatg ctccagataa gtctctcgat caaaaggata
      721 cgcgaaagcg ttagtcgtct gaccatgctg atgtggcatc aaacccgtta acattgtccg
      781 acgcgctggg gcgcacaatg ggcaagtggt gtatgctctc gagaaatcag ccccttgaga
      841 tgcgagtcgc ctgaagttcg gccgttggat ctcgggccct gagtcccaac catgtcggta
      901 atgcagttga tgatcgttga gcaagaagac gatgttcggc ctgcttgaag tctttcgagt
      961 ggcacgggtc ttagaagcct tggttgatgc gcaaccggca aaagttccgg cggcgagagc
     1021 attgaggaaa tcgcgtctgt tcatactgat agtatccttt cgctagcgtg ccagagtata
     1081 gtagcgaaaa cagtctgctg attccagcga gactctctca ataggcatgc tttcttttga
     1141 cagatttgtg cccgagagat agagtgctga tgattgccag caaagagaaa ggaaggatac
     1201 ctttcatgag aaagatgatg cttgcgttca tctcagccgg attcctatgc agtattggag
     1261 tcgcacagga tgtgaatctt gatggtgtca ttgattcagc ggatctcttt gcgctctcca
     1321 gtacctggca catcttcctg aagcagggat cgacccttga ccttatggag gattggcaga
     1381 ccgaggtcac ttatcaaccc gctcgggaga ctgtcgtgct ggagctgacc ccggaaggaa
     1441 acgaaccaca gatctgttta gacggtcgag gtgtcgccaa tgtcgtccat gtcaagagga
     1501 tccctcaact agcaccagac aacagcaccg tgatgacacc ccaactggtt tgttcgagta
     1561 ttctcgcttc tggtgactac tggcagagag aacacgtcct tacagaaaga aagccaaata
     1621 tctacctctg ggggagctac tatgccccgt tactagcgga catagtgctg gccgctccaa
     1681 gagacagggc gggagacttc tacgccgtct ggcctcacta ttccgctggt ttctctatgg
     1741 ttcgtttcaa ccgatatgca ctgccacaga ccaaagagta tcagaccagc acagcaggcc
     1801 tgggaagtat caagcgcgtt gacgccgctg ttgacgatga tggaaacatc cacattgctt
     1861 ggacgactcc cctggttctc aacgcaacga tagaaatccg atacagcaaa ttcacgccag
     1921 ctggcaagat gcttgttgcc ccccctctgt ctctaggaac tcgcttgacg tcttcaggct
     1981 tctcggagct atttgcacct gtgatccgaa tgctcggtgg caagaacccg ctggttctca
     2041 ttgatcaagg ggagtctgcg ccaacgcttg ccgtccgtgt ggacggaacc aatggcacaa
     2101 tacttgaaac gaaagtcctg cctcagaact ttgttgagcc ggactttgtc gagatgccaa
     2161 acggtgatat cgcgttggtt ggcatgattt ttgtgctaaa taaggggcgt gatatcttct
     2221 acactgttct caaaccagat ctaacaaccc ggatagcacc agtgaatttg tccaacacaa
     2281 ctcaacagtc agacggtgcg agtattgctg tcgtcgaaga tgacttctac gtggcctgga
     2341 acgagaacaa agataactat atgcgccttg tacgtctcaa tgccaatgga agcagacaag
     2401 gaaacgtcat cactctgtcc ggtcctggac aggggttccc ttttcctctt cggttggctt
     2461 cggatgctct cggcatggca cacatcgtct acgccgacgg aaagggttcc agtcctgcga
     2521 aagtctctta cgagaagcgc aagatcagat agaggaatcc tgtacagtgt gaatgtggaa
     2581 tcatgtcttg tgttcatttg accggctttg aggactatgc tattcagacg agaaaggtcg
     2641 ttccagtgta ttcgacactg acttgtcaga acacccgcct ttggaaggcg gagagaaatc
     2701 gttcagggaa aaggagactc acacactatg gcatcaaaga agacgacatt cgcattattc
     2761 ttcgggaatc gtggattctt cccagcatca ctccaggcaa gtgctcgcaa ggaaacgacg
     2821 gaacgtcttg agcgattggg tcatcaagtg attgcgttag atcccaaggc aacccgccat
     2881 ggtgcggtcg agactccgga ggaaggcgag aagtacgcac ggttccttca ggaaaataga
     2941 ggcaagttcg gcggcgtgat cctctgcctt cccaactttg gtgatgagac aggcgccgtc
     3001 gctgctctca gagacgccaa cgttcctatc ctgatacagg catatccaga cacatttgac
     3061 aagatggatc cggagcatcg gcgtgatgcc ttttgcggga agttctccat catggacgtc
     3121 tttgttcaat atggagttcc cttcaccgcc ctgaaacccc atacggtgaa tcctcttgga
     3181 gacaagtttc aggagcaggt ggcatatttt gaccgactct gtcgggttgt ccaaggaatg
     3241 cgacggatga tcgttggtgc gattggtgcc cggaccacag cgttcaaaac agtcagaatt
     3301 gatgagatca cgcttcaaga gttcggcatc accgtggaga cccttgatct gtcagatgtc
     3361 ttcgcgcaga tgaaagggat caaggacacc aatcccaagt acaagaagaa ggcaaaagtc
     3421 cttcaagatt actccaactg gaaaggtgta ccggcaaagg ctttcaacac catcgttcag
     3481 ttgggcgttg tcctggacaa tatcatcgaa gagtacaaga tggatgctct ggctcttcgt
     3541 tgctgggtcg aaatgcagag acaactgggt gtttcacctt gtgttctcct cagtgaattg
     3601 aacgatcgag gcattccggc agcctgcgaa gtggacatcg gcaatgctgt caccatgtac
     3661 gggctgagtc ttgcctctgg caaagcggca acctgtcttg actggaacaa caactacggg
     3721 gacgaagacg atcgctgtat ccttttccac tgcggtccgg ttccgaatag catgatgacc
     3781 gcaaagggag atattgaaga ccattccatt cttgccaatg cggtaggcaa gggatgtggt
     3841 ttcggctgta acgttggtcg cattgcccga gttcctttca cctacggaag cctgttgact
     3901 cgcgacggca acattctttg ctatctcggc gagggggcgt tcactgagga cccgattccg
     3961 gaggagtttt tcggttgtgc gggagttgcc gatatcccca atttgcagga cgcgcttcag
     4021 aagataggct atgccggata tcgtcaccac gtcagcgtta cgcctggcca cgtagccgct
     4081 ccaacctatg aagctttcac acggtatctg gggtattgcg tgaccaaagt ctgaaacgaa
     4141 cggacacgac ccgacactga gacgaacggc ttgagaaggt tctaacatgg tctatatcgt
     4201 catgggtgtc tcgggttgtg gcaagacaac ggtgggaaga gtgttggcac aacgcctttc
     4261 ccttcccttt tttgacgcgg attcctttca ttcgccagag tgtattacca agatgaaggc
     4321 aggaattccc ttgtccgatg aagacaggat tccctggttg acgacattgg ccaaacagat
     4381 agaggtatgg cagaggtctg gaggctcagt cctggcatgt tctgcgttga aggaaacat
//
LOCUS       DAOQUC010000137         4438 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_21935_length_4438_cov_3.162446, whole genome shotgun sequence.
ACCESSION   DAOQUC010000137 DAOQUC010000000
VERSION     DAOQUC010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4438
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21935_length_4438_cov_3.162446"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(53..1486)
                     /gene="gatB"
                     /locus_tag="PKH07_03575"
     CDS             complement(53..1486)
                     /gene="gatB"
                     /locus_tag="PKH07_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014956657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HNT34057.1"
                     /translation="MPQYEAVIGLEIHVQLSCPTKMFCDCPNTPGERPNLNTCPICLW
                     MPGAIPQFSQTTLEHAAMTCLALNCEIQSYSKFDQKVYYYPDLPKGFQLSQHHRPLSR
                     NGWLDIVGEDGKPRRLRIHHVHMEEDVAKLVHETEGHTKISLVDFNRAGSPLVEIVTE
                     PDMRSPYEAMEFLKALRAQIRYAGTAECSMENGTMRCDANISLRPKGTDEMNTKVEVK
                     NMNSVRAVGNAILYEIGRQTELLNQGKAITLHTRLWDPDKNVTVPMRGKFSGPCVPDP
                     SVPAICVTPEWIDAMKRRLPEMPGQKKARFVGDYGLTDDEAALMSSERETSEYFETLV
                     RANGASARTAAQWIASRLLPALKERGETPDQTALTPQRMAELLQLLESGEISANAARE
                     VFVLLMNSDKSAAQIVEEHGFKQIGESDELEKMVQVVIEEHPAAVQDFRNGKAQAAGF
                     LIGQVMRASRGKANPKILREILDKKLS"
     gene            complement(1504..2700)
                     /locus_tag="PKH07_03580"
     CDS             complement(1504..2700)
                     /locus_tag="PKH07_03580"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HNT34058.1"
                     /translation="MINELYSYIEPRSGAASKSGSSNGLQICIQPNLNVRGWQTEAGS
                     MALKGYVALDDAYVIERLRAQGAFIAASTRMGELGFGLAGDTTGRAVQQDCDAALVTD
                     AFGESRVIAALHGLLGFKPSHGVVSRRGLIGLIPSMESISVVAKKTGVVSSVLQAIVT
                     PDDQDFSMMQSGLPNFSAPAVPAEEIKTVGVITEAVSLLDAAASKAFQKGLDQVSKKG
                     YAVQKVTLPSFPLFRKAHQVIGSAEASSSAGKFDSVRYGPRASGTSNWNEMYLQTRAE
                     CFGTLLKCYLFQGGYIQFKDFEAFQDACRIRHRLVKEVETLFGKVDALALPTRRDATS
                     PEKAETVEDIYDLFAMTLPANVLGTPAVSLPGFVDSDGVDLGLQLIAPRVRDRQLLEM
                     AAALVG"
     gene            complement(2744..>4438)
                     /gene="aspS"
                     /locus_tag="PKH07_03585"
     CDS             complement(2744..>4438)
                     /gene="aspS"
                     /locus_tag="PKH07_03585"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HNT34059.1"
                     /translation="YRYLDLRRPSMQNNLIKRYEILKQARQVLDSRGFIEVETPDLTK
                     STPEGARDYLVPSRVHPGCFYALPQSPQLFKQLLMVSGMERYFQVVRCFRDEDLRPNR
                     QPEFTQLDIESAFIDEEFIYDLIEEIVVRMFKVGGVDLPRPFPRMTYQDAIATTGSDR
                     PDLRFGLRFVHATDIFKKTTYSIFKQVLSRGGHILGINVKGQSEALSKNVLQNEYAKE
                     IVPSFGGKGMTWMRVTEGRLDSNIVQFFSEAEQAQVLERFECEEGDVLMLVADPSYRV
                     ATSVLGQLRLHVAHRLGLVPSGTYCPVWVTDFPLFDPTEEGGVTSTHHPFTSPDRTDF
                     DPKNVDELLSLKSRAYDIVLNGEELGGGSIRINDPIVQAKIFAALGLTDEDVEQKFGF
                     FIRALDYGAPPHGGIALGVDRVVSMILDTQSIRDVIAFPKNRSAFCPLTSAPSPVARE
                     QLAELGLLNLGGGEVLPGSGHETVLDSVSWVCRIGLRDGEMNAICVALENVQLLTKAV
                     REHAGDEEPLYSVTSADPVFRAGKEARVHPHAQSGELMKNAPAVKGDYFKVVNILE"
BASE COUNT          986 a         1291 c         1163 g          998 t
ORIGIN      
        1 cttctgcaca gagacacagc cgtatcgctg agccagtaca cataccacgc tctcaactca
       61 gtttcttgtc gaggatttcg cgcaggatct tcggattggc tttgccccgt gaagcacgca
      121 tcacttgtcc gataaggaaa cccgcagcct gcgccttccc gttgcgaaag tcctgcactg
      181 cagcggggtg ttcctcaatg acaacctgca ccatcttttc caactcgtcg gactcaccga
      241 tctgtttgaa cccgtgttct tccacgatct gagcagcgga tttgtccgaa ttcatgagga
      301 gcacaaaaac ttcgcgggca gcattggcgc tgatctcacc agactccaga agttgaagaa
      361 gttccgccat cctctgcggt gtcaatgccg tctgatcggg agtttctcca cgctccttga
      421 gtgccggaag aagtcttgat gcaatccact gggcagcggt tcgggcggag gctccgtttg
      481 ctctgacaag cgtctcgaag tattcgctcg tttcgcgttc tgaactcatg agcgccgcct
      541 cgtcgtctgt gagaccgtaa tccccgacaa atcgggcttt cttctgtcca ggcatctcgg
      601 gaagccgacg cttcatcgcg tcaatccact cgggagtgac gcaaattgcc ggaacagaag
      661 gatcgggaac gcagggcccc gagaacttgc cgcgcatggg aaccgtaacg ttcttgtccg
      721 gatcccaaag tcgagtgtgg agcgtaatcg ccttgccctg attcagaagt tcggtctggc
      781 ggccgatttc gtagaggatg gcatttccaa cagcccgaac cgagttcatg ttcttgacct
      841 cgaccttggt gttcatttcg tcggtgccct tgggtcggag agaaatgttc gcgtcgcagc
      901 gcatggtgcc gttttccatg ctgcattcag cggtgcccgc atagcgaatc tgtgctcgca
      961 acgctttgag aaactccatg gcctcatagg gacttctcat gtcgggttcc gtaacgatct
     1021 cgaccagcgg agaaccagca cggttgaaat caacaagact gatctttgtg tgtccctcgg
     1081 tttcgtgaac cagtttcgcc acatcttctt ccatgtgaac atgatgaatt cgaaggcgtc
     1141 gtggttttcc gtcttcaccg acaatgtcca accatccgtt gcgggacaag ggacgatggt
     1201 gctgcgacag ttgaaagccc tttggcagat cgggatagta gtagaccttc tgatcgaact
     1261 tgctgtacga ctgaatctcg cagttgagcg ccaggcatgt catcgccgcg tgttcgaggg
     1321 ttgtttgact gaattgcggg atcgctccag gcatccagag acagatcgga caggtgttca
     1381 gattcggcct ctctccaggg gtgttgggac agtcgcagaa catctttgtc ggacaactca
     1441 actgcacatg tatttccaaa cctatgactg cttcatattg aggcattgtt tctctcctta
     1501 cattcatccc acgagggctg ctgccatctc cagcagctgt cgatctcgga ctcgcggcgc
     1561 aatcagctgc aaccccagat caactccgtc tgaatcaaca aaaccaggca gactcaccgc
     1621 tggcgttccg agcacattgg cagggagagt catggcaaag aggtcataga tgtcctcaac
     1681 ggtttcggct ttctcaggac tcgttgcgtc tcgccgtgtt ggaagcgcca gagcatccac
     1741 tttgccaaag agggtttcga cctccttgac cagtcggtgt cggattcgac aggcatcttg
     1801 aaatgcttcg aagtccttga actgaatgta accgccctga aacaggtagc atttgaggag
     1861 cgttccaaaa cactctgccc ttgtctgaag atacatctca ttccagtttg aagttcctga
     1921 agcgcggggc ccatatcgaa cgctatcgaa cttgcccgca ctggaagagg cctcggcaga
     1981 accgatcacc tgatgcgcct tccgaaagag gggaaaactc ggcagcgtca ccttctgaac
     2041 cgcatagccc ttctttgaga cctgatcgag gcctttctga aatgccttgc tcgctgctgc
     2101 atcgagcaga ctcacggctt ccgtgatcac cccaacggtc ttgatctctt ccgcggggac
     2161 agcgggcgcg gagaaattcg ggagaccact ctgcatcatc gagaaatcct ggtcgtccgg
     2221 cgtcacgatc gcttgaagga ctgaagatac gacacctgtc tttttcgcaa caacggaaat
     2281 gctctccatc gaggggataa gaccgatgag ccctctgcgg gacacaacgc catgactcgg
     2341 tttgaacccc agaagaccat ggagcgcggc gatcacacgg ctctcaccaa aagcatccgt
     2401 gacgagagcg gcgtcgcagt cctgttgaac tgcccggcca gtggtgtctc ccgccagacc
     2461 gaaacccagt tcacccattc gagtgctggc cgcgatgaaa gcaccctgtg cgcgcagtcg
     2521 ctcaatcaca taggcatcat caagagccac gtagcccttg agcgccatag aacccgcttc
     2581 cgtctgccaa ccacgaacat tgaggttggg ctgaatacat atctgcagcc cgttcgagga
     2641 gccggacttt gaggcggcac cggagcgcgg ctcgatatat gagtaaagct cgttgatcat
     2701 tcatttcctc ctcgacaaac gctctggagt ctttgcatgt cctctactcc aggatgttga
     2761 caactttgaa ataatccccc ttgacggcgg gcgcattttt catgagttca ccggactggg
     2821 catgaggatg cactctcgcc tctttgcccg cgcgaaaaac cggatcggcg gacgtgacgg
     2881 agtaaagagg ctcctcgtca ccggcatgtt ctcggacggc ctttgtgaga agctgaacat
     2941 tctccaaagc aacacagatc gcgttcatct caccgtctcg aagtccaatc cggcagaccc
     3001 atgacacaga atccagaacg gtttcatgtc ctgagccagg cagcacctct ccgccaccga
     3061 gattcagcag ccccagttcc gccagctgtt cccgcgccac cggagaaggt gcgctggtca
     3121 gcggacagaa ggcactgcgg ttcttcggaa aggcgatcac gtcccgaatg gattgcgtgt
     3181 ccagaatcat cgaaacgacc cgatccacac ccagcgcgat gcccccgtga ggtggcgctc
     3241 catagtccag cgctcggata aagaatccaa acttctgttc cacatcctca tcggtaagtc
     3301 ccagcgcggc gaatatcttc gcctgaacaa tcggatcgtt gatacgaata ctgccaccac
     3361 caagttcctc tccgttgagc acaatgtcat aagcccttga cttgagggag agaagctcat
     3421 cgacgttctt cggatcaaag tcggtgcggt cgggcgaggt gaagggatgg tgcgtggacg
     3481 tcactcctcc ttcttccgtg gggtcaaaga gcgggaagtc cgtcacccag acgggacaat
     3541 aagtccccga gggtaccaaa ccgagccgat gtgccacgtg cagtcgtaac tgccccagaa
     3601 ccgaagttgc cacacggtat gagggatctg cgaccagcat cagcacgtca ccttcttcgc
     3661 actcaaaacg ctcaagcacc tgcgcctgct cggcctcact gaagaactgg acgatgttgg
     3721 aatccagtcg cccctcagtg acgcgcatcc acgtcatccc cttgccacca aaactcggga
     3781 cgatctcctt ggcgtactcg ttttgcagca cgttcttgct cagtgcctcg gactgccctt
     3841 tgacgttgat gccaagaatg tgcccccccc gactcaagac ctgcttgaag atggagtaag
     3901 tggtcttctt gaagatgtcc gtcgcgtgaa caaaccgcag cccgaaacga agatccggac
     3961 gatcagatcc ggtcgtcgca atcgcatcct gataggtcat tcgcggaaat ggtcttggaa
     4021 gatcaacgcc gccaaccttg aacattcgca caacgatctc ttctatcaga tcgtagatga
     4081 actcctcgtc aatgaacgcg gattcaatgt ccaactgtgt gaattccggc tggcggttgg
     4141 gacgcaagtc ctcgtcgcgg aagcagcgta ccacctgaaa atagcgttcc attccgctga
     4201 ccatcaacag ttgcttgaac agctgaggcg actgtggcaa ggcgtagaaa catccgggat
     4261 gcacacgact gggcaccaga taatcacgcg cgccttcggg cgtgctcttg gtcaagtctg
     4321 gtgtttcgac ttcgatgaaa ccgcggctgt cgagcacttg tcttgcctgt ttcaggattt
     4381 catagcgttt gatgaggttg ttctgcatcg aaggtcggcg cagatccagg taacgata
//
LOCUS       DAOQUC010000138         4431 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_21978_length_4431_cov_3.454068, whole genome shotgun sequence.
ACCESSION   DAOQUC010000138 DAOQUC010000000
VERSION     DAOQUC010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4431
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21978_length_4431_cov_3.454068"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3396
                     /locus_tag="PKH07_03590"
     CDS             <1..3396
                     /locus_tag="PKH07_03590"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNT34060.1"
                     /translation="DPIRRHVNGVNPPKRMWRSLFGTMLAFEAEIATHMGLDAFTFFT
                     PFDARTRSFTPVDTLDRMNIPNVSRQVETIFYLLSEMIKTRNVFPEAKFMVDDYGRDV
                     WGKVYEFDRKKRFVPEDKIPSAFVTYRHSEDWMRHTRDVCGVKTDQILFSSIVDATPG
                     LLKEEIEPGDFYIRNVVVVDETLNPKPGITFAGYVLGDDGEIVMMPDLGQDGARSYPL
                     GTGTFFFLNKAMVMLFPCRTMTLYDIIDSRYLRALDVFDIKTGYDAEPIRYGKAEHPW
                     QFYRPKDIEPIIVVAAEPDERIKILGSTGPYGVKYLLTNAPEELLTNPVPAADITNEI
                     EEQSRGTGYPLTASLDHSAYKAARDMWIIDDIRIKSFSRYGIRNERLMRLHEQAREAL
                     LSAKAFLEQYRYDSFMSAVQRAWGLEARGYPDVKATMNDTVSGVLFYFAILVPFSVFM
                     ERLIFGFPDIRKRVAGFAFFLLLIFLILRKVHPAFKLSTSPYVIFLAFIIMMLAGVVL
                     SIIVTKFGDEIRKMKASGTGVSDVDVGRLSAAKAAVFIGISNLRKRKVRTVITALTVT
                     LLTFTVQSFTSVRSTMRFYKIPVSETPSYQGGLIRDLNWAPLQECTLDYLRGAFAHCA
                     RVIPRCFNSVRSQGQMEYFRVQNTETNKATLAFGLVGLTPEEIHAIDYPSAFVGKSRW
                     FETATERSCILPDTMASLLGLSETDIGRASVNIMGQEFDLCGILDAQKYNSLRDLDDE
                     KTTPLDTVAQMSEARQAADTSDVGKAALKLESFWHIDADNTVILPFDWVMDHGGVLES
                     ISVTAFRNPDGTPLENFIPIVEDFMLRIATTMFVGYQNSVKVYSSIGQTSFSGVGRLL
                     VPIAIAAFIVLNTMMGSVHERRKEISVYSSVGLAPTHIGALFVAESAVFATVGAVLGY
                     LLGQVVMRLLTDFGWLSGLELNYSSISAVYSTLIVMVVVFLSSLYPARLASSMAVPDV
                     TRKWKFPDPVGDLWHFDFPFTVRGSDALAMCVYLAQYFRAYTESSLGSFFARDVGLTG
                     TENDEPHYTIEMMIWLAPYDLGVSQRVRLEAFSTGEYGIYKIVITITRESGQVTTWRR
                     MNRGFLNTLRKRCLVWRTITPAQRDKYREEGIRLVGSSAGVAE"
     gene            3444..>4431
                     /locus_tag="PKH07_03595"
     CDS             3444..>4431
                     /locus_tag="PKH07_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide transporter"
                     /protein_id="HNT34061.1"
                     /translation="MRDKLGLEYDARDFQIMPPDAPFEAGFNMKTIVAALFVGLVILP
                     GSIYLGLITGVGLGGAANWVTIILFIEIAKRSLVRLKTQEIIILYWVSGGLAIAAGGH
                     ALFGGPLGAKIWDQYLIQSPQAAGIARFIPDWVVPARGSEALIERTFFHRDWLVPLLL
                     ILGLAILGPINSLSLGYVLFRITNDVERLPFPMEMIHAAGATALAETSNKREGWRWRV
                     FSIGAIIGVVWGCIYVVVPTLSGLFLRETIMILPIPFIDLTASLKEILPAAVLSIATD
                     LGALLVGFVIPFWIVIGSFVGGFVKNLLVDPLLYHYGVLYRWQPGMTLIPTNL"
BASE COUNT          886 a         1105 c         1272 g         1168 t
ORIGIN      
        1 gatcccattc ggagacacgt gaacggtgtc aacccaccca agcgcatgtg gcgcagtctg
       61 tttggaacga tgctcgcatt cgaggcagag attgcgactc acatgggctt ggacgccttc
      121 acatttttca ctccatttga tgcgcgaact cgcagcttca ctcccgttga cactcttgac
      181 aggatgaaca ttcccaacgt ttcgagacag gtcgagacca ttttctacct gctctcggag
      241 atgatcaaga ccagaaatgt ttttcccgaa gccaagttca tggtggacga ctatgggaga
      301 gatgtgtggg gcaaggttta cgagttcgac cgcaagaagc gatttgttcc tgaggacaag
      361 attccgagtg cttttgtaac ataccgacac tcggaagact ggatgcggca cactcgggat
      421 gtatgtggcg taaagaccga tcagattttg ttttcctcga ttgtggacgc cactccagga
      481 ctgctgaagg aagaaattga gccgggggac ttctacatca gaaatgttgt cgtcgtagat
      541 gagacgctta accccaagcc agggatcact ttcgccggat atgttctcgg ggatgacgga
      601 gagattgtga tgatgcccga cttgggccag gatggtgccc gaagctatcc cctcggaaca
      661 gggactttct tctttctcaa caaggcgatg gtcatgttgt tcccctgccg aacgatgacc
      721 ctctatgaca tcattgattc ccgctacctt cgcgctctcg atgtcttcga catcaagacg
      781 ggctatgatg ccgaaccgat acggtacgga aaggcggagc atccatggca gttctaccgt
      841 cccaaagaca ttgagcccat catcgtcgtg gctgccgaac cggatgaacg aatcaagatt
      901 ctgggcagca caggtcccta tggcgtcaag tacctgttga caaatgcacc tgaggaactg
      961 ttgaccaatc ctgtgcctgc agccgatatc accaatgaga tcgaggaaca gtcacggggg
     1021 acaggttacc cactgactgc gtcgctggat cactccgcct acaaggcagc cagggacatg
     1081 tggatcattg atgatatccg gatcaagagt ttctcccgct atgggattcg caacgaacgc
     1141 ctcatgcggc ttcacgaaca agcgcgagaa gcactgctgt cagcgaaggc ctttctggag
     1201 caataccgat acgactcgtt catgtccgcc gtccagagag cgtggggttt ggaggctcga
     1261 ggctatccgg acgtcaaggc aaccatgaac gataccgtca gcggggtgct gttttacttc
     1321 gccattctcg tgccattttc cgtctttatg gaacggttga tcttcgggtt tcctgacatt
     1381 cgaaagcgcg ttgccggctt tgcgtttttt ctcttgttga tctttctgat tcttcggaag
     1441 gttcatcccg ccttcaagtt gagcacttcg ccctatgtca ttttcctggc gtttatcatt
     1501 atgatgttgg ctggagtggt tctgagcatc atcgtcacaa agtttggtga tgagattcgg
     1561 aagatgaaag cgtcgggtac gggggtcagt gatgtggacg ttggccgatt gagtgccgcc
     1621 aaggcggcgg tcttcatcgg catttccaat ctgagaaagc gcaaggtgcg aactgtcatc
     1681 acagcattga cggtcacgct tctgacgttc accgtgcagt cgtttacttc cgttcgctcc
     1741 acaatgcgat tctacaagat ccctgtttca gagactcctt cctaccaggg tgggctgatt
     1801 cgggacttga actgggcgcc actgcaggaa tgcacactcg actaccttcg gggcgctttt
     1861 gctcattgtg cgcgggtcat tccccgctgt ttcaactccg tgcgcagcca ggggcagatg
     1921 gagtatttca gggtgcagaa caccgagacc aacaaagcaa cgctggcatt tggtcttgtc
     1981 ggtctgactc ctgaggagat tcatgccatt gactatccgt cggcgtttgt cggaaagagt
     2041 cgctggtttg agactgcaac ggagcgcagc tgcattttgc ccgatacgat ggcttcgttg
     2101 ttgggtctgt cggagacgga tatcggccgt gcatcggtca atatcatggg gcaggagttt
     2161 gatttatgcg gcatcctgga cgcgcagaag tacaactcgc tccgagatct ggatgacgag
     2221 aagaccactc ccttggacac cgttgcgcag atgagcgaag cgcggcaggc tgccgatact
     2281 tccgatgtgg gaaaagcggc gttgaagttg gagtcgttct ggcacattga cgccgataac
     2341 acggtcattc ttccttttga ctgggtcatg gatcatggtg gagttctcga atccatttcg
     2401 gtcacggcgt ttcgcaatcc tgatgggact ccattggaga acttcatccc tatcgtcgag
     2461 gatttcatgc tgcgcatcgc gacgacaatg tttgttggat atcagaactc ggtcaaggtc
     2521 tacagttcca tcggtcagac aagtttctcc ggtgtgggac gacttctggt acccatcgcc
     2581 atcgcggcat ttatcgtgct caatacgatg atgggttcgg ttcacgagcg tcgaaaggag
     2641 atcagtgtct acagttctgt cgggctcgcc ccaacgcata tcggagcctt gtttgtagcc
     2701 gaatcagcgg tgtttgcgac tgtgggagct gttctgggct atcttctggg acaggtggtc
     2761 atgcgattgt tgaccgattt cggttggctt tcgggtttgg aactgaacta ctcatccatt
     2821 tcggcggtct attccacgtt gatcgtcatg gtcgtggttt ttctttcttc tctttaccct
     2881 gctcggctgg cctcctcgat ggcggtgccg gatgtcacgc ggaaatggaa attccctgat
     2941 ccggtgggcg atctgtggca ttttgacttt ccgttcactg ttcggggttc tgatgctctt
     3001 gcgatgtgtg tttatctggc gcagtatttt cgggcttaca ccgaatcgtc cctgggatca
     3061 tttttcgcgc gcgatgtggg actcactggg acagagaacg atgaaccgca ctatacaatc
     3121 gaaatgatga tttggctggc tccctatgac ttgggtgtca gccagcgtgt gcgacttgag
     3181 gcgttttcca cgggtgaata tggcatctac aagattgtca ttaccatcac tcgggagagc
     3241 ggacaagtca ccacatggcg gcggatgaac agaggttttc tcaacacgct caggaagcgg
     3301 tgcctcgtct ggagaacgat cactccagca cagagagaca agtatcggga agagggcatt
     3361 cggcttgtcg ggagcagtgc aggcgttgct gagtaggtcg cctgaacttc aagggtggaa
     3421 tgatggcaga taaaattcag gagatgcgcg acaaacttgg tctggagtac gacgccagag
     3481 acttccagat catgccgccc gatgcgccct ttgaagcggg cttcaacatg aagaccattg
     3541 tcgcggctct gtttgttggt ctggtgattt tgcccggttc tatctacctt gggttgatta
     3601 ccggagttgg cctggggggt gctgccaact gggtcacgat cattctgttc attgagatcg
     3661 ccaagcgttc tttggttcgg ctgaagacgc aggagatcat cattctctac tgggtgtccg
     3721 gggggcttgc catcgccgcc ggtggtcacg ctttgtttgg cgggcctctg ggcgcgaaaa
     3781 tctgggatca gtatctgatt cagtcgccgc aagcggcggg catcgctcgt ttcatcccag
     3841 actgggtcgt tccggcgaga ggttctgagg cgctcatcga gagaaccttc tttcatcgcg
     3901 attggttggt tccgctgctt ctgatactgg gactggcgat tcttgggccc atcaacagtc
     3961 tgagcctggg ttatgtgctg ttccgaatta ccaatgatgt tgagcggctg ccgtttccta
     4021 tggagatgat ccatgcggcg ggtgccacgg cactggcaga gacctccaac aagcgcgaag
     4081 gctggcgctg gcgtgtgttc agcattggag cgatcattgg tgttgtctgg ggttgcatct
     4141 atgtggtggt gccgacactc tccggcctgt ttttgcgcga gacgatcatg attctcccga
     4201 ttccgtttat tgatctgacg gcttcgctga aggagattct tcccgcggcg gttttgagca
     4261 ttgccaccga tctgggcgct ctgctggtgg gattcgtcat cccgttctgg attgtcatcg
     4321 gttcctttgt tggtgggttt gtcaagaatc tcctggtgga tccgctgctt taccactacg
     4381 gcgttttgta tcggtggcag cctggaatga ctttgattcc gaccaatctg t
//
LOCUS       DAOQUC010000139         4417 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_22062_length_4417_cov_3.107978, whole genome shotgun sequence.
ACCESSION   DAOQUC010000139 DAOQUC010000000
VERSION     DAOQUC010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4417
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22062_length_4417_cov_3.107978"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..651)
                     /locus_tag="PKH07_03600"
     CDS             complement(<1..651)
                     /locus_tag="PKH07_03600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34062.1"
                     /translation="MKDTGKRLLLSVTLVILVLLTSRCGREQAAPVSEPTATPVPEQV
                     QVIETATPTSIPPTPEPTATPTEEPEVEITYLLEDFEERLPGVWAFKSDAMGQVSVET
                     NIVHSGQQAARVEWDFSRRPKDDPGFVNYFFHSEMVGSPEMAKVWIHSATTESAPGSV
                     QLWIEDNSGEVFLTQATVEKTGWSQLVFKVSEASANWTSGDGNGNKDLPLRFFGLAV"
     assembly_gap    1014..1023
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1024..3616
                     /locus_tag="PKH07_03605"
     CDS             <1024..3616
                     /locus_tag="PKH07_03605"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 78 protein"
                     /protein_id="HNT34063.1"
                     /translation="NPLLSWTLSSDGHGARQTAYQVLVASGPELLKSGRADIWDSGIV
                     PSDHSQAIGYSGIPLVSKTDYFWRVRVWDQRAKPSRWSQTAHWTTGFLHDEAWQGEWI
                     AMPSYSNGDAPLLRKTVTLEKRPRKALIFVSGLGYYELYVNGKKVGDRVLDPGFTDYT
                     KRVFYVAWDVTDLLQKGSNAIGVMLGGGWFHVATPELFGFEKAPWTAPPKLRLEMDMA
                     FPDGTNRVLGSDTTWKCSTGALEFNCIRAGETWDARLDKPDWNLPEFGDTDWEHAEIT
                     EPPQGKMIAQTHPPIRAVETIQPVSVSEPKPGIYVFDLGVNIAGWVSFKTSGTEGQKI
                     TLQFNEKLSPDGTVDMRHTSGHTYGRFQTGEFILKGEGVETWEPRFTYHGFQYIQVSG
                     LLEKPSLDALTGKCVHTDPEPAGAFACSDPRINKIQEVLRRTQLNNLHGIPTDCPQRE
                     KMGWLHDGCVTVEEAIFNWNMANFYTKWYRDLIDAQEANGHIPSIVPTCGWGQSGANG
                     EPHAFSDPWWGGAIVLVPWRLYLYYGDRNILAEAYPAMKNYLRFLESAAQDDIIHWHL
                     GDWLEQGAGGGSRRTPVALSSTSAWFLMAKIAGDVARILDRAEEAEQYDTTADRIKEA
                     FNARFLSRETGHYAEESQMAQSVPLLYDMVPEELKEAVTERLIENIVEVRKGHVSTGI
                     VGTWPLFECLTKIGRSDLAFEMLVKEDYPGWLWMINNGATTLWEAWDDVSSRNHPAFG
                     CVGAWFYQVLAGIRPDPDRPGFRHIVIQPEPVGDLSWVKARYESAQGLIVSEWEKSDG
                     RFTLSLSLPANTSAIVSLPASSNSTVLESGKPASNASGVLDDLGFENGRRAFRVASGD
                     YCFQVSQ"
     gene            complement(3641..>4417)
                     /locus_tag="PKH07_03610"
     CDS             complement(3641..>4417)
                     /locus_tag="PKH07_03610"
                     /inference="COORDINATES: protein motif:HMM:NF025086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HNT34064.1"
                     /translation="DREAEVLQKNKRGIFSLDVKPIGRRQFLGSVAVGLGAFAAVARA
                     EDEPLPDEDQGFPLFARLKFPGGDWYTDTLTGGHAGGAELNLLRRFRKNTTGKAIVSE
                     TFVDPMTDEIFKYPFLYMSGHTASDFPQVMVDNLRHHVLCGGMLLADECSGMQNGSFE
                     MACRRLFIRMLPEQALRPLSIDHPVYHCAYDVNEVLGGDKLVSPYDTAVELDGRIGVF
                     YCANDYGCAWEGHRCVPKGDIQREHAFKMGINLLVYALTH"
BASE COUNT         1046 a         1212 c         1142 g         1007 t
ORIGIN      
        1 gaccgcaagt ccaaagaacc gaaggggcag atccttgttg ccgttgccgt cgccgcttgt
       61 ccagttggca ctcgcctcgg aaaccttgaa aaccaattgg ctccatcctg tcttttcgac
      121 tgtcgcctga gtcaaaaaga cctctccact attgtcttca atccaaagtt gaactgagcc
      181 tggagcagat tctgtggtgg cgctgtgaat ccaaaccttg gccatttctg gactgcccac
      241 catctcgctg tgaaaaaagt agttcacgaa tcccggatcg tctttgggac gcctggaaaa
      301 gtcccactcc actcgagctg cttgctgccc actgtgaacg atgttggttt ccacactgac
      361 ctgacccatt gcatcggact tgaatgccca tacgcctggc agtctttcct caaagtcctc
      421 caacaaatag gtgatctcca cttcgggttc ctctgtcggt gtagcagtcg gctctggggt
      481 ggggggaatg gacgttggag tggccgtctc aatgacctgc acctgttcag gcacgggggt
      541 tgcagttggc tcggaaacgg gtgctgcctg ttctctgcca caccgcgagg tgagcaagac
      601 caagatcacg agcgtcactg aaagcaacaa ccttttgcct gtgtccttca ttgcagtctc
      661 cctttcgctc cattcagaat gggcaactcc cgaacgaggg tttctgaccc cacagcggga
      721 taagcctgtg ttgatcaaac cgagatcact ttctttcaat gtatcgcaca ctgcgcttta
      781 caatcaactg atgactgctc gcgtgcagat gctgacatac tcttgccttc ttgggtttga
      841 ccttgtgccc ggacgcgtgt accttctcgg catcaaatcc agaggaagga agattcgcac
      901 atgatttcct gcaatcgact tctgatactg ttacctttcg ttctcgcttt tgtccacacg
      961 gcagcagtgg ctgcctcgct gaaacctgag aaccttcggt gtgagcgcct gccnnnnnnn
     1021 nnnaaacccc cttttgagtt ggacgctcag ttccgatggg cacggggcaa ggcagaccgc
     1081 ataccaggtc ttggttgcct caggaccgga actcctcaaa tcgggaagag cggatatctg
     1141 ggactcagga atcgttccat ccgatcattc acaggcaatc ggatactcag gtatacccct
     1201 ggtttccaag acagattact tctggcgtgt ccgagtctgg gatcaacggg caaagccttc
     1261 acgatggagt cagaccgctc attggacaac cggctttctt cacgacgagg catggcaggg
     1321 agaatggatt gccatgccaa gctattccaa tggtgacgcc ccgcttctca gaaagacagt
     1381 cactttggaa aaacgaccca gaaaggcgct catcttcgtt tctggactcg ggtactatga
     1441 actctacgtc aacggaaaga aagtcggcga cagggtcttg gatccgggtt tcacagacta
     1501 caccaagcgc gtcttctatg tcgcgtggga tgtgacggac ttgcttcaga aaggctccaa
     1561 tgcaattggc gtcatgctcg gtggtggttg gtttcacgtg gcgacacccg aattgttcgg
     1621 ctttgagaag gctccatgga cagctccgcc aaaactccgt cttgagatgg acatggcctt
     1681 tcccgacgga acaaacaggg ttctcgggag cgacacaacc tggaaatgct ccaccggtgc
     1741 gcttgaattc aactgcatcc gggcaggcga aacctgggat gcacgtctgg acaagcccga
     1801 ctggaacttg cctgagtttg gcgacacaga ctgggaacac gccgagatca ctgaacctcc
     1861 ccaagggaag atgattgccc aaacgcatcc gcccatccgc gcggtggaga ccattcagcc
     1921 agtaagcgtc tcggagccaa aaccgggtat ctatgtattc gatctcggtg tgaacatcgc
     1981 tggctgggtc agtttcaaaa cgtccggaac ggagggacag aaaatcacac tgcagttcaa
     2041 cgaaaaactc tctccagacg gaacggttga catgcgtcat acatcaggcc acacgtatgg
     2101 cagatttcag actggtgagt tcatcctcaa gggcgaaggt gtggagacct gggagcctcg
     2161 atttacctat cacggatttc agtatattca ggtgtctggc ttgttggaaa aaccctcact
     2221 ggacgcactc actggaaagt gcgttcacac cgatccagag cctgctggag cattcgcgtg
     2281 ctctgatcct cgaatcaaca agatccagga agtccttcgc agaacgcagt tgaacaacct
     2341 gcatggcatc ccaactgact gcccccaacg tgagaaaatg ggttggctgc acgatggatg
     2401 tgtcaccgtt gaggaagcca tcttcaattg gaacatggcg aacttctata ctaagtggta
     2461 tcgagacctg attgacgctc aggaagccaa tggtcatatt ccctctatcg tgccgacctg
     2521 cggatgggga cagtcaggtg ccaacggaga gcctcacgca ttctccgatc cctggtgggg
     2581 cggtgccatc gtcctcgttc cctggcgact ctatctctac tacggcgatc gaaacatcct
     2641 tgctgaagcc tatccggcaa tgaagaacta tctgagattt cttgaaagcg cagcacagga
     2701 tgacatcatt cactggcatc tcggagactg gttggaacag ggtgccggag gtggcagtag
     2761 gcgcaccccc gtggctctca gctccacatc cgcctggttc cttatggcaa aaattgccgg
     2821 tgacgttgcc aggattctcg atagagccga agaagcagag cagtatgaca ccactgcaga
     2881 tcgaatcaaa gaggcgttca atgccagatt cctttcgcgc gagaccggac attacgccga
     2941 ggaaagccag atggctcagt ctgttccctt gctgtacgac atggttcctg aagaactgaa
     3001 ggaagccgtc acggaacgct tgatcgagaa catcgttgaa gtgcgaaaag gacacgtcag
     3061 caccggcatc gttggaacat ggcctctctt tgagtgtctg accaagatcg gtcgcagtga
     3121 tcttgccttc gaaatgcttg tcaaggaaga ctaccctggc tggctctgga tgatcaacaa
     3181 cggcgctacc acgctgtggg aagcctggga tgatgtttca tcacgaaatc accccgcatt
     3241 tggttgcgtc ggagcatggt tctatcaggt cttggcgggc atccgcccgg atcccgaccg
     3301 acctggattc aggcatattg tcatccaacc agagccggtt ggtgatctct cctgggtcaa
     3361 agcaagatac gaatctgctc aagggcttat tgtgagcgaa tgggagaagt ctgatgggcg
     3421 attcacattg agtctctctc tgcctgccaa cacatcggcc attgtctccc ttccggcaag
     3481 ctcgaattca actgtacttg aaagcggcaa gcctgcttca aacgcttcag gcgtactgga
     3541 tgacttggga tttgagaatg gacgaagagc attccgagtc gcatcgggag attactgttt
     3601 tcaggtaagc cagtaggtag cctatgagca acgcagtctt tcagtgcgtc aacgcataga
     3661 cgagaaggtt gattcccatc ttgaaggcat gttcacgttg aatatcgccc ttcggaacac
     3721 aacgatggcc ttcccaggcg catccgtaat cgttggcgca gtagaagacg ccaatacgac
     3781 catccagttc aacggcggtg tcatacggag aaaccagttt gtccccacca agaacttcgt
     3841 tcacatcata cgcacaatga tacacaggat gatctattga aagaggacga agcgcctgct
     3901 caggaagcat tctgatgaaa agacgcctgc acgccatctc aaaagatccg ttttgcattc
     3961 cggagcactc gtccgcaagc agcatcccac cacagagaac atggtgtctg aggttgtcca
     4021 ccatcacctg gggaaaatcc gacgcagtgt gcccgctcat atagagaaac gggtacttga
     4081 aaatctcatc cgtcatcggg tctacaaagg tctcgctgac aatggctttc cctgtggtgt
     4141 tttttcgaaa tcgccgaagc agattcagtt ccgcaccacc ggcatgaccg cctgtcagcg
     4201 tgtccgtata ccagtcacca cctggaaact tcagacgcgc gaacaacgga aagccctgat
     4261 cttcatcagg cagcggttca tcttcagctc gggcaaccgc ggcaaatgca ccaagcccaa
     4321 cggcaacgct ccccagaaat tgacgtcgtc caatcggctt gacgtcgaga ctgaagatcc
     4381 ctctcttgtt tttctgcagg acttccgctt ctctatc
//
LOCUS       DAOQUC010000140         4404 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_22150_length_4404_cov_3.026443, whole genome shotgun sequence.
ACCESSION   DAOQUC010000140 DAOQUC010000000
VERSION     DAOQUC010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4404
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22150_length_4404_cov_3.026443"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..565
                     /locus_tag="PKH07_03615"
     CDS             <1..565
                     /locus_tag="PKH07_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HNT34065.1"
                     /translation="CLRAILRQDPDVIMVGEIRDLETAQIAIESALTGHLVLSTLHTN
                     DAPSAVTRLVDMDVEPFLVTSSVVGVLAQRLVRTICPNCRDEYRPKEDLFYDLGLSSD
                     DIKGVTFTRGRGCAECNYVGYRGRIGIFELFQLSEHLREMILKREPSSVIRRQARLEG
                     MRTMREEGWTKIKMGLTTVEEVLRETT"
     gene            complement(614..>883)
                     /locus_tag="PKH07_03620"
     CDS             complement(614..>883)
                     /locus_tag="PKH07_03620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34066.1"
                     /translation="RRTQKHNGILSQVDLQEVEVRARSAYREMQDAWRFQHVLPNHDG
                     EDSEHWDAFYYPIGDARQLLLSFVALLRKEPAPLAEKWDQKTLPI"
     assembly_gap    884..893
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<894..1350)
                     /locus_tag="PKH07_03625"
     CDS             complement(<894..1350)
                     /locus_tag="PKH07_03625"
                     /inference="COORDINATES: protein motif:HMM:NF012832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34067.1"
                     /translation="MPELVVVSGPSCVGKSPLSKALRMFYPDIVAKLEPLVLMNSRAP
                     RPGERDGVDYHFRARGFIQSLQSQSGFLVIESRSDLQAVNLTDVERILDRGKSPFFEG
                     NPYVGKALISARELDAVEKMTVFVSPLAKEEIASLSAPQKQVDMENLITD"
     gene            complement(1395..3365)
                     /locus_tag="PKH07_03630"
     CDS             complement(1395..3365)
                     /locus_tag="PKH07_03630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34068.1"
                     /translation="MRHVKQKELDLSTPNHALTNRAFVLGLLCVAFTSLLAPASLLIY
                     RSSLLACDYFPLGAILPFFFIVAGVNVALKFVGRHYALTPRELTLIFMMALISTTIPT
                     FGLVCYMLSVSSSPYYYASPENRFDEYIFPNLKSWAVVPDINNTVKWFFEGIPKGEAI
                     PWGPWIVPVLWWVSIALMAFFMCFFLVVILRKQWMENENIAYPLTEIPLTMVEGSDDQ
                     SLLPRFMRSRIFWSGFAIPFCILLWNLANRIWPTFPSISLNRRQPVGTVTVDFLMKFP
                     VIGFTYFVNTGAAFSIWFFWVLGLFQAHIFARLGISTFVGEPYCEGHPPIVFQEWGAF
                     IAMVAMAVFMARGHVLKVFKKAFFGGISLDDSKELTSYRTAVWGFLICFVLLTIGLCI
                     TGLKLYMALIFVLCVLIIYIGITKIVIECGIVNLRSPMLPQVFMVNALGTSQIGMQGL
                     TALASNYTWFSDIKSVFMPAAAHSERIRHVQGIGRREVLIALFSSMALAIGLSIWFII
                     YSGYQKGAYNWNTWIFQYGAYGPYDQMVRKFRDVKFAKPQWTLLLWTVSGAVIYVLLN
                     IMRYRFTWWPLHPVGMTVNATFGVMWNAFSVFLGWACKAAILKIGGAPLYQKCRPFFI
                     GLILGHFVGVSIAFLVDVFYFGPGGGAMWHGW"
     gene            3490..3939
                     /locus_tag="PKH07_03635"
     CDS             3490..3939
                     /locus_tag="PKH07_03635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34069.1"
                     /translation="MRLNLSPKELRTLVDFAAVLDSIFMVEGEFSTSKKTVPHKAMIQ
                     KILSLAHESQLTDLVTLDEEGESFFPTEKCLGEFAEPLMEEFTAESFWEELIQRLGTR
                     DFSEQMTEKSIQEMPEEELFMRLQECVSKYSTEMFEHGLSRLKIIEP"
     gene            3936..>4404
                     /locus_tag="PKH07_03640"
     CDS             3936..>4404
                     /locus_tag="PKH07_03640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34070.1"
                     /translation="MRFPSVSRNLRTAILVGAIAALANGLIWIHGFCGGFSKLELHEA
                     DQMVYTSLARNLANGQGFVDSCIYPVSLSVVQRIPHPYLRGTLFPMMLVPFFVLFGAR
                     DLWAIVPVWCCYGLLVAAAFLMARRVLSLRSSLLACVLVATNPVVIRLSVRCLP"
BASE COUNT         1076 a         1160 c         1156 g         1002 t
ORIGIN      
        1 gtgtcttcgt gccattttgc gccaagaccc cgacgtgatc atggtgggtg aaatccgtga
       61 cttggagaca gcccagatcg ccatcgaatc cgctctgacc ggacaccttg tgttgtccac
      121 tcttcacacc aacgatgcac cctcagccgt gacccgcttg gtggacatgg acgtcgagcc
      181 ttttctggtt acgtcctcgg ttgtcggcgt tctggcgcag aggcttgttc gaacgatctg
      241 cccgaactgc cgagatgaat acagaccgaa agaagacctg ttctacgacc tcggtttgtc
      301 ttctgacgat atcaaaggtg tcacatttac ccgcggccgt ggttgtgccg agtgcaacta
      361 cgtgggatac cgaggacgaa tcggaatatt cgaactgttt caactctccg aacatctccg
      421 ggagatgatc ctcaagaggg agccgagctc cgtgattcgt cgtcaggcac gtcttgaggg
      481 gatgcgcacc atgcgcgagg agggttggac taagatcaag atgggtctca ccactgttga
      541 agaagtcctt cgagaaacca cttagcgttc accctttggc aaaggcgttt ccatttctgt
      601 ctgttctcgg cattcagatt ggcagagtct tctgatccca tttctcagca agcggtgcgg
      661 gttccttacg caacaacgcg acaaaagaca agagaagttg gcgcgcatct ccaatcggat
      721 agtagaaggc gtcccagtgt tcgctgtctt ctccgtcgtg gttgggaagc acatgctgga
      781 atctccaggc atcctgcatt tcacggtaag cactgcgggc acgaacttcg acttcctgca
      841 agtccacctg tgagagaatg ccgttgtgtt tctgagttct tcgnnnnnnn nnncatcggt
      901 gatcaggttt tccatgtcca cctgtttttg tggtgcgctt agcgaggcga tctcctcttt
      961 tgccaggggg gagacgaaga cggtcatttt ctcgacggcg tccagttccc tcgccgagat
     1021 gagggctttt ccgacatagg gattgccttc gaaaaaggga ctcttcccgc gatccaagat
     1081 gcgctcaacg tccgtgagat tgactgcctg cagatcgctt cgggactcga tcaccagaaa
     1141 ccccgactgt gactgaagcg actggatgaa tcctctggct ctgaagtgat agtccacgcc
     1201 gtcccgttca cccggtctcg gagcacggct gttcatgagc accagaggct ccagcttggc
     1261 gacgatgtcc ggatagaaca ttcgcagtgc cttgctcaag ggactcttgc cgacacacga
     1321 cgggccactg acaacaacga gttcaggcat gatcctctcc ttcttcagca acagcagtca
     1381 tctccgattt tgcgtcacca accgtgccac attgctccac cgccgggacc aaaatagaac
     1441 acatccacca gaaaggcaat gctgacacca acaaaatggc cgagaatcag accaatgaag
     1501 aacggtcggc acttctgata caagggggca ccgccgatct tcaggatagc ggccttacat
     1561 gcccagccga ggaaaaccga aaaagcattc cacatgacgc caaacgttgc gttcaccgtc
     1621 atcccaacgg gatgcagagg ccaccacgtg aaacgatatc gcattatgtt cagaagaaca
     1681 tatatcacgg cgccggaaac cgtccataga agcagcgtcc actgtggttt ggcaaacttc
     1741 acgtccctga atttgcgaac catctgatcg tacgggccgt aggctccata ctggaaaatc
     1801 cacgtgttcc agttgtaggc acccttctga tagccgctgt agatgatgaa ccagatggaa
     1861 agaccgatgg ccaaagccat tgacgagaac agcgcgatga ggacctctcg acggccaatt
     1921 ccctgcacat ggcgaatgcg ctccgagtgc gccgcggcgg gcataaacac ggatttgatg
     1981 tccgaaaacc atgtgtagtt tgacgccagg gcggtcagcc cctgcatgcc gatctggctg
     2041 gttccgagcg cgttgaccat gaacacctgt ggcagcatgg ggcttcgaag attgacaatt
     2101 ccacactcga taacgatctt ggtaattccg atatagataa tcagaacgca taggacaaag
     2161 atcaatgcca tgtagagctt cagtccggta atgcagagtc cgatggtcaa caagacaaag
     2221 cagatcagaa atccccagac cgccgtcctg taactggtga gttccttgga gtcatccagc
     2281 gatatgccgc cgaagaatgc cttcttgaag accttgagaa catgtcctcg tgccataaag
     2341 acggccattg ctaccatggc gatgaatgca ccccattcct gaaagacgat gggtgggtgt
     2401 ccctcgcagt aaggttctcc gacaaaggtt gatatcccca gtcgcgcaaa gatgtgcgcc
     2461 tgaaaaagcc ccagaaccca gaagaaccag atgctaaacg cagcccctgt attgacaaaa
     2521 taggtaaatc caatgaccgg aaacttcatc aggaagtcta ccgtcactgt tccgacaggt
     2581 tgtcggcgat tcaaactgat gctggggaag gtgggccaga tccgatttgc caagttccag
     2641 agcaggatac agaacggtat tgcaaagccc gaccagaaga tacggcttcg catgaacctt
     2701 ggcagcaaac tctggtcgtc ggaaccttcc accatcgtca gtggaatctc agtcaaaggg
     2761 taggcaatgt tctcattttc catccactgt ttgcgaagaa tcaccaccag aaagaagcac
     2821 atgaagaatg ccatcaaagc gattgacacc caccagagta cgggaacaat ccaaggcccc
     2881 cacgggattg cctcgccctt gggaatgcct tcaaagaacc atttcacggt attgttgatg
     2941 tctggaacca ctgcccagga cttcaggttg ggaaaaatgt actcatcaaa acggttttcg
     3001 ggacttgcgt agtaataggg cgatgaactc accgagagca tataacaaac caaaccaaag
     3061 gtcggtattg tcgtggaaat cagtgccatc atgaaaatca gggtcagctc tcgtggagtc
     3121 agcgcatagt gtctgcccac gaacttgagc gcgacgttca cgcccgcaac gatgaagaag
     3181 aagggcagaa ttgcaccaag agggaagtaa tcgcacgcca gcagggaaga gcgatagatc
     3241 aggagcgaag ccggtgccag caggctcgta aaagccacgc agagcagacc caggacaaat
     3301 gcccgattcg tcagtgcgtg gttcggtgtc gacaagtcca gttctttctg cttcacatgt
     3361 cgcatgaacg taaggtaact tcattgatgc atgtttgtca aagcaagtcg atctgtcgtt
     3421 gtgaacctgt tggcatttcc aaatggggcg tggtattctc gttcaaatct tcttggaaag
     3481 gacgatcaca tgaggctcaa tctcagtccg aaagaactaa gaacgcttgt tgactttgcc
     3541 gctgttctcg acagcatctt catggtggaa ggggaattct ccacgtcgaa gaagactgtg
     3601 ccacacaagg caatgataca gaagatactg tcacttgctc acgaatctca gttgaccgat
     3661 ctggtgaccc tagatgaaga aggtgagtcc ttcttcccaa ctgagaagtg tcttggagag
     3721 tttgccgaac ctctaatgga agaatttacg gctgagtcct tctgggaaga gctcatccag
     3781 cgtctgggca caagggattt ctcagaacag atgactgaaa agagcatcca ggaaatgccg
     3841 gaagaggaat tgttcatgcg cttgcaggag tgcgtttcca agtattccac ggagatgttt
     3901 gagcacggcc tgtcgcggct gaagatcatc gagccatgag attcccctcc gtctccagga
     3961 atctacgaac ggccattctc gttggcgcaa tcgcagcgct cgccaatggc ttgatctgga
     4021 ttcatggatt ctgcggggga ttctcaaaac ttgagcttca cgaagcagat cagatggtct
     4081 atacgagcct ggctcgaaac ctggcgaacg gtcagggctt cgttgattcg tgtatctatc
     4141 cggtttctct atccgttgta cagcgtattc cgcaccccta tctgaggggc accctgttcc
     4201 ccatgatgct ggtgcccttt tttgtcttgt ttggtgctcg cgacctgtgg gcgattgttc
     4261 ctgtttggtg ctgctatgga ctgctcgtgg ccgcggcttt tctgatggcg cgtcgagtgc
     4321 tttcactcag gagcagtctg cttgcttgtg tgttggtggc gaccaatcca gtcgtgattc
     4381 gactgagtgt ccgatgcctt cccg
//
LOCUS       DAOQUC010000141         4395 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_22220_length_4395_cov_2.879954, whole genome shotgun sequence.
ACCESSION   DAOQUC010000141 DAOQUC010000000
VERSION     DAOQUC010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4395
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22220_length_4395_cov_2.879954"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(440..1978)
                     /locus_tag="PKH07_03645"
     CDS             complement(440..1978)
                     /locus_tag="PKH07_03645"
                     /inference="COORDINATES: protein motif:HMM:NF012936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HNT34071.1"
                     /translation="MADWQHETASGTGLKDPQDSGNCESDFFGGLDFAGLHSNLLSAA
                     AANATQYQGMDSLQWAWLTQRRDHIPFPRQSVNARGVVVLLAGGVDNLDELADRYLRW
                     ASPEEPLPQDPHEQAPFQIGQLYEQLGIGFLERLRGPFAIVLWESRRQRLLLARSPSG
                     QRTLYYAQSGSRFTFSTNLGMAARLENVTTQLDRGALYSYLVMGGVPAPETIHQNVCA
                     VASGTWLVFSETGLQSGRFWTVPPLPRWHIGIREAEKLLDEKLSDAISLALKPIRRVG
                     CLLSGSLNSSALVFFARKFQRKGQSLSTYTLARRYCSWREFRIAAQVADLCRTQHVPI
                     GMYADPVTTVLDLVACLDAPSSEPSHLRSFLMMRSPQITADGLLAGFGGDEILAGRPY
                     HAMLNVPPPLNLFRSFLGGFPLESSLGERLIPESAEEAYLLLTQRFSAQEAQALLGGE
                     FSAQSFCSSPADPRTDLLRTFLDRDVLHRLPDLMLPALDGFARSAGRILRLPFLDQEV
                     VELP"
     gene            complement(1936..2418)
                     /locus_tag="PKH07_03650"
     CDS             complement(1936..2418)
                     /locus_tag="PKH07_03650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34072.1"
                     /translation="MILKSFLKSKWAMALILLGFVLILWREIHPNTPEHQIQRTLLKA
                     AKAFQAESIRQCEPFISFSYEDEYGQDKRLLLANAGSFFEQVEGLEVRILHTQITLSE
                     ETSFATSEVYFELSGTLEGTRFQGLLSSEEEPDIAIIDWHRTSGKWQIGSMRLLREQD
                     "
     gene            complement(2469..3662)
                     /gene="galK"
                     /locus_tag="PKH07_03655"
     CDS             complement(2469..3662)
                     /gene="galK"
                     /locus_tag="PKH07_03655"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HNT34073.1"
                     /translation="MSEETGANNKLSALQERFQQVYGQPKGTWVAVSAPGRVNLIGEH
                     TDYNEGYVFPVAINTTVDVMAEAVSGNTVEIFAVGFDEKKTFSVNEREPHSTHCWVEY
                     PRGVVILLRQRFSSISGMRMVIQGNVPLGAGLSSSAALEVATAFAVKELFGLDINLKD
                     LALLCQKAEHLFAGVNCGIMDQFASALCEEGAALLLDCRNQETQAVPLPTNQFSLVIV
                     NSMVKRGLVDSEYNTRRRECEQAVETLKRIYRKRKIHSLRDVTAEDWRQWKRDLSGKP
                     QLRAQHVITENDRVLRCVQALKEGDMETFGTLLYEGHGSLKTDYEVTVRELDLLVGFA
                     RQSRLVWGARMTGAGFGGCVVNIAKRGTEDQLCALLAEEYNRATKTVPEFYRCEPSPG
                     VRRIG"
     gene            complement(3723..>4395)
                     /locus_tag="PKH07_03660"
     CDS             complement(3723..>4395)
                     /locus_tag="PKH07_03660"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HNT34074.1"
                     /translation="EEYTLVPETHAYRLTSNVGIEGGILAETLACVLHGYDRLNLRAG
                     SSALILGAGCVGLLWTELLRKSPITTLIQTEIIDFRAKLAEELGAEIVINPTRDNLKK
                     AVLQASPGGVDYLIDATGDATPIQEALPLVRKGGTVMLFGVCPSQQSLAVEPFEMFNR
                     ELTIMGSKMPPATLERAARLVESGLISQKKLVNKVLPLSKISAGVELFHKGKSETVKI
                     AVVPD"
BASE COUNT         1027 a         1236 c         1131 g         1001 t
ORIGIN      
        1 aaacctgatg gtggatcctg gtcaatttat cctgccatgg ccatcagcct tcccaatacg
       61 atcaaagctc tgggggttac cgaggctaat ttgaatgacg aattcacagt tgcctccagt
      121 tttgatgaaa ctcttccgat tactctttcg tcctttacgg ccacggtcaa ctcccaaaat
      181 atgctgaccc tggcctggag taccgcgtcc gaaacctcaa tgcagggtta taacgtgtac
      241 tacaagccgg caatctcaaa tccggagatg atctgcttaa cgccagttct aattccagcc
      301 gaaaacagca gcagcggatc ccattacagc ttcgtagctg atgatttgac cgaaccggga
      361 acctattact tctggttgga agcgatttcg atcgacggca gcagtgagtt tttcggccct
      421 gtgaatgcta ccttgtcagc tacggaagtt ccaccacttc ctgatcgaga aaaggcagtc
      481 ggagaatccg acctgccgaa cgcgcaaagc cgtccaaggc cggcagcatc aagtcaggca
      541 gtcggtgcag gacatctctg tccagaaatg ttctgagaag atcggttcga ggatcagccg
      601 ggctgctaca gaacgactga gcactgaatt caccacccaa caaggcctgt gcctcttgtg
      661 ccgagaatcg ctgggtcaac aacagatagg cctcttctgc tgattctgga atgagccgtt
      721 ctccaaggct gctctccagg ggaaagccgc caagaaagga tcgaaagaga ttcagcggtg
      781 gtggaacatt cagcattgcg tgatacggtc tacctgcaag aatctcatct ccaccaaatc
      841 cggccagcag accatcggct gtgatctgag gggaccgcat catcagaaaa gatctgagat
      901 ggctgggttc tgaactgggg gcatcaagac aggcgaccag atccaagacg gttgtgacgg
      961 gatccgcata cattccgatg ggcacatgtt gggttctgca tagatcggca acctgtgctg
     1021 caatgcgaaa ctctcgccag gaacagtacc ttcgggcgag tgtgtatgtg gagagcgact
     1081 gtcctttgcg ctgaaacttt ctggcgaaga agacaagggc acttgagttg aggcttccgg
     1141 aaagcaaaca tcccacacga cggatcggtt tcagcgccag agagatcgca tcggataact
     1201 tctcgtcaag cagtttctcc gcttcgcgga ttccaatgtg ccaccttggc agtgggggaa
     1261 ccgtccagaa tcgtcctgac tgtagtcccg tttccgagaa aacaagccac gttccagaag
     1321 caacggcaca gacgttttgg tggatcgtct cgggtgccgg gactccaccc atcaccagat
     1381 aggaatagag tgcccccctg tccagttgtg ttgtgacgtt ctcaagtcgc gctgccatgc
     1441 ccaggtttgt cgagaaggtg aatctactgc cggactgggc gtagtagaga gttctttgac
     1501 cggatggact gcgcgccaag agcagccttt gtctgcgcga ctcccagagg acaatagcaa
     1561 aaggcccgcg gagtctttcg agaaagccga tcccgagttg ttcatagagc tgcccgattt
     1621 ggaagggggc ttgttcgtgt ggatcctggg gaaggggttc ttccggtgaa gcccagcgca
     1681 gatagcggtc ggcaagttca tcgaggttat ccacgccacc agccaagagc acgacaacac
     1741 ccctggcgtt cactgactgt cgcggaaagg ggatgtggtc tcttctttga gtcaaccaag
     1801 cccactgaag agaatccatg ccctgatatt gtgtggcgtt ggcagccgcg gctgaaagaa
     1861 ggttggagtg caaacccgca aaatcaagac ccccaaagaa atccgactcg cagttgcctg
     1921 agtcctgtgg gtctttcagt cctgttcccg aagcagtctc atgctgccaa tctgccattt
     1981 tccactcgtc ctgtgccagt caatgatagc gatgtccggc tcctcttcag atgacagcag
     2041 accttggaat cgtgttccct cgagcgtgcc ggaaagctcg aaatagactt cgctggtcgc
     2101 gaaagaggtc tcttcactca gtgtgatctg agtgtgaagg attcttactt caagcccttc
     2161 cacctgctca aagaaggatc ctgcattcgc cagaagcagc cgcttgtcct gcccatactc
     2221 gtcttcgtaa ctgaaggaga tgaagggctc acactgacga atactctcgg cctgaaatgc
     2281 cttggctgcc ttcagcagcg ttctttggat ttggtgctct ggggtgtttg gatggatttc
     2341 tctccagaga atcaggacaa agccaagcag gataagcgcc atagcccact tggatttgag
     2401 aaaggacttg aggatcatct tcccgccgct ttcatcgcca cgctctttgg gaaaccactc
     2461 cttgtcactc agccgatgcg ccgaacaccc ggagatggtt cacaacgata gaattctggc
     2521 acggtcttgg tggctcggtt gtactcctca gccagcagag cgcataactg gtcctcagta
     2581 cctctcttgg caatgttgac gacgcatccg ccaaagcccg caccggtcat tcgagcgccc
     2641 cagacgagtc gggattgacg tgcaaaccca acgagcaaat ccaactcgcg tacagtcact
     2701 tcatagtctg tcttcagact tccgtgccct tcgtagagca gagtcccaaa agtctccata
     2761 tcaccttctt tgagcgcttg aacacatcgc agcacacgat cgttttccgt aatgacatgc
     2821 tgggctcgca actgcggttt gccggaaaga tcgcgtttcc actgacgcca gtcttctgcg
     2881 gtcacatcgc gcagcgagtg aatcttccgt tttcgatata tccgcttcag tgtttcgact
     2941 gcctgttcac attcccgtcg ccgcgtgttg tactcggagt caacaagacc tcgcttgacc
     3001 atggaattga caataacgag gctgaactga ttggtgggaa gaggaactgc ctgggtttcc
     3061 tgattccggc agtccagcag cagtgcagcg ccctcttcac aaagcgcact ggcgaactga
     3121 tccatgatgc cgcagttgac cccagcgaag aggtgctcag ctttttgaca cagtaaagca
     3181 agatccttca gattgatgtc cagaccgaac aactccttga ccgcaaaagc agtggccact
     3241 tccagggctg ccgaacttga gagtcccgcc cccaagggca cattcccttg gatgaccatt
     3301 ctcatacctg aaatgctgga gaatcgctgt cgcaagagga ttacgacgcc tcgtggatac
     3361 tcaacccagc agtgcgttga gtgaggctcc cgctcattga ccgaaaaggt cttcttttcg
     3421 tcaaaaccaa ctgcaaaaat ctcgacagtg tttcccgaaa ccgcctcagc cataacatca
     3481 acggtggtgt tgattgccac cggaaagacg tacccctcat tgtagtccgt atgctcgcca
     3541 ataaggttta cccgtcctgg cgcgctgacg gcaacccagg tccctttcgg ctgtccgtat
     3601 acttgctgaa atcgctcctg caaggcgctc agtttgttgt tggctcccgt ttcctcactc
     3661 acgacacaat cctcccgatc gtcattggcc tgtcaagaag agagacttca cgtgcgcaat
     3721 cctcagtccg gcacaacagc gattttgacc gtctcggact ttcccttatg gaaaagttcc
     3781 accccagccg aaatttttga aagtggaaga accttgttga cgagtttctt ctgagagatc
     3841 agacccgact caaccagacg cgcagcccgc tccaaagttg ccggtggcat cttggagccc
     3901 atgatggtca actccctatt gaacatttcg aacggttcaa cagccagact ctgttggctc
     3961 ggacaaacgc caaagagcat gaccgtacca cccttgcgca ccaggggcag cgcttcctgg
     4021 ataggtgtgg catcgccggt agcgtctatc agataatcca cgccgccagg agaggcctgc
     4081 agcaccgcct ttttgagatt gtccctggtg gggttgatga caatctccgc accaagttcc
     4141 tcagccagtt tggctctgaa gtcaatgatc tcggtctgga tcagcgttgt aatcggactc
     4201 ttccggagca gttccgtcca gagcagtccc acgcatcctg cacccagaat caaagcggac
     4261 gaacccgcgc gaagattcag tcggtcgtag ccgtgcaaca cacatgcgag ggtctcagcc
     4321 aaaatacccc cttcgatgcc gacattggag gtcaaacgat aggcatgtgt ttcgggaacc
     4381 agtgtgtatt cctcg
//
LOCUS       DAOQUC010000142         4364 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_22448_length_4364_cov_2.304711, whole genome shotgun sequence.
ACCESSION   DAOQUC010000142 DAOQUC010000000
VERSION     DAOQUC010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4364
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22448_length_4364_cov_2.304711"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            227..2059
                     /locus_tag="PKH07_03665"
     CDS             227..2059
                     /locus_tag="PKH07_03665"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA14 domain-containing protein"
                     /protein_id="HNT34075.1"
                     /translation="MNQSEPLDNDLELSQTRCRKPRELTFRVELACLAFVLIWAFAVR
                     VYHLNEVPVNLMCDEADNMGDCLKVLYGKGPGIFGFDWKPQPAFSIYLMTAFLRVLGV
                     HIWSVRFPSVTLSILALIPLYYLVKRATSQIAALAATFLMANGIWYLHFSRSAWENVH
                     ICLYSLMAFLLLEKAVSSRKWWFAYFAGSGFFAGIGILGYFAGKFILPSLLLVFPLHI
                     LIHRKQWKRIVTGYAIIVVVALGVSAPHLKIARLPQNREYAARRIANVYLFNNPEGKG
                     PLVEVLTRQIKKNIQGFFDPQMNNQPRYGRVRHTPLDYATGAFFACGAIISLFHLRKS
                     WIWWSMFLTQFVFGQILTRGTPDPARGVPMIPTIYFLVALSIDLFEPYWYGFWQRRVP
                     VILCLLCLIVAWYNVSSYFEWCYTDDAIRNRDPMVETDEFPYWLEAQRELLQNQRGPF
                     NLDQWKQRLRAQVLEKVRPSPKTYSGDGGLTMTLFGNPSWQGAPTRQERKAPVALPSD
                     APEGAYSIRWKGRMLIERQGKYTFGLNSDDGSELLINGKRIVDNRGQHGAQERMGSII
                     LQAGEHDVEIRYFQSDGASRLDFWWKPPLTDKEPVPLGILIPEP"
     gene            2127..>4364
                     /locus_tag="PKH07_03670"
     CDS             2127..>4364
                     /locus_tag="PKH07_03670"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNT34076.1"
                     /translation="MNLGRFFKIPDKASRIVRVIGLAVALGLAGYGQWHMADLQLRQG
                     LLFYLCAGILSVVVLEYRSSSLVYGAPEKDDKSPLDPPLLRGAGPFLPPFGKGDRGDS
                     SESRKSLSAGRQMPSWVELLLLLSIMAVAAWFRLHRILEIPAGFFVDECHQAYEAVRV
                     MRGELRGAFRTGWYQVPTLYFYYVSVFFRILGISVWVMKFSTVCSAVLTIIPLYLLAR
                     QWYGKGIGLTLAGFLATNRWHLTMSRWGMVEVLPPLLGTAAIYFMVRGVSKGSEFPLP
                     LRFRNEDPPEGCDDCSSPLPSIWARLVFFFLSGTMLGLGLYTYLAARLLPPVCAFFMI
                     FVMLTRKRSVRSYVLACVVLVVAFCIVFGPLALHFQKYPNEFSNRTNEISIFEDSEGK
                     FVWQPVWTSIQKHLLMFNYRGDTNGRHNLPGEPMFSHGLSMLFVLGLFLCLGRMDKPW
                     RMLLLIAFCFTMAGGAFSSLREAPQAFRTLTVVPVALLIAGVFVDSAKRFLQETIQCP
                     GGRLSWICIVAGWLVPVGLLGWSSVQDFDTYFHRQGLSPEVTVHYNMEATFVARRLTE
                     MKRDDTIYLFEGYQANSICQFLNHDATNHYRFKRIIHLPLFPFEEGDIFIFGPPRWDY
                     LEKVLKLFYPNSTMTRKLDPHNGGLMYLEFHIPKSDWNQVAGARWEVRDASSDSVLST
                     RVVSQMGVLPDYAKETLGTDAERALSYRFIANLFCQNYREYNFSVLKQAGSGDRGGEE
                     ISVLFN"
BASE COUNT          889 a         1079 c         1198 g         1198 t
ORIGIN      
        1 gtttagaaag aacgcccttg ttaagagggg ttccggaaac acccccctta ctaagggggg
       61 atcaaggggg gattcagagt agagcagtgt tcgtcgagcg gagcggatgc cccagaccac
      121 gttgaatgca gtccttcgga atgttcgctg ttggcatgga ctgcgctctt gattgctttt
      181 acttctctgg taatatgatt tcactgtgat ttcgcaagag gaaactgtga atcagtcgga
      241 acctttggac aacgacttgg agctgagtca gactcggtgt cgaaagccgc gcgaactgac
      301 ttttcgcgtg gagttggcgt gtcttgcctt cgtcttgata tgggcatttg ctgttcgcgt
      361 ctatcacctg aacgaagtgc cagtcaacct catgtgtgat gaagcggaca acatgggcga
      421 ctgcctgaaa gttctttacg gcaaaggacc gggaatcttt ggattcgact ggaaacccca
      481 gcctgctttc agcatctatc ttatgaccgc ttttctcagg gtgctgggcg tgcatatttg
      541 gtccgttcga tttcccagcg ttacattgag cattctcgct ctgattcctc tctactatct
      601 tgtcaaaagg gcaacgtccc agattgcagc gttggcagca acttttctca tggcaaacgg
      661 catctggtac ctgcatttct cacgaagcgc gtgggagaac gttcatatct gcctgtactc
      721 gctgatggct ttccttttgc ttgagaaggc agtctcgagt cgcaagtggt ggtttgctta
      781 ttttgccggc agtggttttt ttgcgggcat cgggattctt ggttacttcg cgggaaagtt
      841 catcctgcca tcgttgctgc ttgtgtttcc gcttcatatt ctgattcaca gaaagcaatg
      901 gaagcggatt gtcaccggat acgccatcat cgttgttgtg gctctgggcg tttcggctcc
      961 ccatctcaag attgcccgcc ttccacagaa ccgcgagtat gccgcccgcc gaatagccaa
     1021 cgtctatctc tttaacaacc ctgagggcaa gggaccgctg gtggaggttc tgacacgcca
     1081 gattaagaag aacatacagg gcttcttcga tccgcagatg aacaatcagc ctcggtacgg
     1141 cagagttcgc cataccccgc ttgactatgc gacgggggct ttttttgcgt gcggtgccat
     1201 catcagtctt ttccatctca gaaagagttg gatctggtgg agcatgttcc ttacgcagtt
     1261 tgtctttggt cagattctga ctcgtgggac tcccgacccg gcgagaggcg tccccatgat
     1321 tccgacgatc tactttctcg ttgcgctcag cattgatctc ttcgagccct attggtatgg
     1381 attctggcaa cgaagggttc cggtgatcct ctgtctgctt tgcttgattg tggcctggta
     1441 caatgtcagc agctattttg agtggtgcta cacggacgac gcaatcagga atcgggatcc
     1501 gatggttgaa acagatgagt ttccctattg gcttgaggca cagcgtgagc tgcttcaaaa
     1561 ccaacggggt ccattcaacc ttgaccagtg gaaacagagg cttcgggcgc aggtactgga
     1621 gaaggtgcgt cccagtccga agacatactc cggagacggt ggcttgacga tgaccctttt
     1681 tggaaatcca tcctggcaag gagcaccaac tcgccaagag aggaaggctc ctgttgctct
     1741 tccaagtgat gcaccggaag gagcatacag catcaggtgg aaaggccgta tgcttatcga
     1801 gagacaaggc aaatacacct ttgggttgaa ttccgatgat ggatctgaac tgctcatcaa
     1861 tgggaagagg atcgttgaca atagaggaca gcacggcgca caggagcgca tggggagcat
     1921 catcctgcaa gccggagagc acgatgttga gatccgctac ttccaatcgg acggtgcaag
     1981 tcgtcttgat ttctggtgga agcctccttt gactgacaag gaaccggttc cattgggaat
     2041 cttgataccg gagccttaag agtgttttgt cgtggccaca gcgccgcgtt tccatctcag
     2101 agtgttctcg agccgatgag gtcttgatga atttgggacg atttttcaaa attccagaca
     2161 aggcgagtcg gatagtccga gtcatcggtc ttgctgtcgc gcttgggctt gcaggctatg
     2221 ggcaatggca catggctgat ctccagctcc ggcaaggctt gctcttttac ctctgtgcgg
     2281 gcatcttgag cgttgttgtg cttgagtacc gaagctcctc attggtttac ggcgcacctg
     2341 aaaaggatga caaatccccc cttgatcccc ccttgttaag gggggcagga ccgttcttac
     2401 cccccttcgg taagggggac aggggggatt ctagcgagag caggaagtct ctgtcagcag
     2461 gacggcagat gccttcgtgg gtggaactgc tgctgctcct ttccatcatg gcggtcgccg
     2521 cttggtttcg gcttcaccgg atcttggaga ttcccgccgg tttcttcgtt gatgagtgtc
     2581 atcaggcgta tgaagcagtt cgagtcatgc gtggggagct ccgaggcgct tttcgaaccg
     2641 gttggtatca agttccaaca ctctacttct actacgtttc agtctttttc aggattctag
     2701 ggatttctgt ctgggtgatg aaattcagta cggtttgttc agcagtactg acgatcattc
     2761 ccctgtatct tcttgcacgt caatggtacg gcaagggcat tggcttgacg cttgccggat
     2821 ttctagcgac caaccgatgg cacttgacta tgagtcgttg gggtatggtc gaggtgctgc
     2881 cgccacttct tggaaccgca gcgatctact tcatggtccg tggcgtgtca aaaggctccg
     2941 agtttccttt acccctgcgc tttcggaatg aagatccacc ggaagggtgt gacgattgct
     3001 cgtcaccact gccctcgatt tgggctcgcc tggttttctt cttcctgtct ggcacgatgc
     3061 tcggtctcgg tctctatacc tatctggcgg cgaggctgtt gccccctgtg tgtgcattct
     3121 tcatgatctt cgtcatgctg acgcggaaaa gatctgttcg ctcctacgta ttggcatgtg
     3181 ttgttctggt tgtggcattt tgcattgttt ttggtccact ggcgcttcat ttccaaaagt
     3241 atccgaatga gttctcgaat cgaaccaatg agatcagcat ttttgaggac tctgaaggga
     3301 aattcgtctg gcagcccgtt tggacgtcca tccagaaaca tctgcttatg ttcaactaca
     3361 ggggagatac gaatgggaga cacaatctcc cgggcgagcc aatgttcagt catggactct
     3421 ctatgctttt cgtcttgggt cttttcctgt gccttggtcg catggacaag ccgtggcgga
     3481 tgctcttgct catcgccttt tgcttcacga tggccggtgg ggcattcagt tctctgcgcg
     3541 aggcacctca ggcgtttcgg actctgaccg ttgtgcctgt tgctctcctc atcgcgggag
     3601 tttttgtaga cagcgccaag agatttcttc aagagacgat acaatgtccg ggcgggcgtc
     3661 tttcgtggat atgcattgtt gccggatggc ttgttcctgt aggtcttctt gggtggtcct
     3721 cagtacagga ttttgacaca tacttccata gacagggcct gagtcctgaa gtcaccgttc
     3781 actacaacat ggaggcgacg tttgttgcgc ggcgtctgac cgagatgaag agggacgaca
     3841 cgatttactt gtttgagggc tatcaggcga actccatctg ccagtttctc aaccatgacg
     3901 cgacgaacca ctatcgcttc aagagaatta tccatctgcc tctctttccg tttgaagaag
     3961 gtgacatttt catttttgga ccaccacgct gggactatct tgagaaggtg ttgaaactgt
     4021 tctatccgaa ttccacgatg acgagaaaac tggatcctca caacggtgga ctgatgtatc
     4081 ttgagtttca tattccgaag tccgactgga atcaggttgc tggagcgcga tgggaagtcc
     4141 gggacgcatc gagtgacagc gttctgtcaa cccgtgtcgt ctcgcagatg ggtgttttgc
     4201 ctgactacgc aaaggagact cttggcactg atgctgaacg ggctctctct taccgattca
     4261 ttgctaacct tttctgtcag aactatcgtg agtacaactt ctctgtcctg aaacaggcgg
     4321 gaagtggcga ccgtggcgga gaagagatct cagtcctgtt caac
//
LOCUS       DAOQUC010000143         4351 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_22538_length_4351_cov_4.271648, whole genome shotgun sequence.
ACCESSION   DAOQUC010000143 DAOQUC010000000
VERSION     DAOQUC010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4351
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22538_length_4351_cov_4.271648"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1388
                     /locus_tag="PKH07_03675"
     CDS             <1..1388
                     /locus_tag="PKH07_03675"
                     /inference="COORDINATES: protein motif:HMM:NF012688.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HNT34077.1"
                     /translation="VLIEQLRNASPYTFTNYKRTFPLTFPALYGNFEGLKIDQEKRTQ
                     LRETAEIKLKKARQRLQTMFADPNFNPGSWQQVEHYVYGIFGAIKPKVGKSKSGTDEK
                     NLKVVGEQHPFLLRMTEEILEYRENQKAIGTYYDFLQLKGRLMWALDPFGTDTTRMAC
                     KASSFWVGTQVQNVPGYAKEMLIADEGFEIFEADNKQSEGRTTAYCSQEEALILALED
                     AHRDFYKTLGTLFFDIPYEEVTDFFRNKVLKRIVHGTNYMMGAKTFIENIGVRILLET
                     AGKLGIVIVETPRANRPNHRTLHQFAKDLLDAYHKPFPRVREWYKEIEEEIRDTGFLV
                     SPTGQVRRFFGDIRRQHTMLRSAVAHQPQQLSVEILNKGFLRAYHEICLPSNGDFRLK
                     AQIHDSIFGQWRISRRDEFAQKLADCMYNPTVIHGRTLVIPIDIKYGQNWAEADESNP
                     NGTVKYKGLKK"
     gene            1511..2629
                     /locus_tag="PKH07_03680"
     CDS             1511..2629
                     /locus_tag="PKH07_03680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34078.1"
                     /translation="MNFGIGNIYPNQYIMLMGAPGTRKGTAMSIGKKLLKAAGYTRFA
                     KDKTSKERFLMDMKHIDESSAMLEDLEVLAMDTPAESYVMAPEFTDWIGQGNMDFVMF
                     LTNVWDNLDKYEHPKIHGKSVEVFKPTVNLLGCNTAEGFALAFPPEALGTGFLSRTLM
                     LYAEVTGKRVAFPEPTDELLLHDLANRMIEVRKNIKGEIVLNEDGRAAAKEIYENEIP
                     VDDPRFTHYQQRRYIHMLKIATLLSVYNSKEKITDIEIVRANTALVAAEKLMPKALGE
                     FGASRYSMVSGKILAYLNTCTLPQNSNDLWKVVSRDLTKMTEMIDILQGMQVAEKIQK
                     IEIAGKSGYLPLRKASKTWPDYLLDLQWLTETERIILS"
     gene            2626..3585
                     /locus_tag="PKH07_03685"
     CDS             2626..3585
                     /locus_tag="PKH07_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008058517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HNT34079.1"
                     /translation="MNLLNQDNNLSLVAFKNPLVIYHGNCADGFSAAWVFHHMQDKIE
                     TAFDFHAGVYGKPPPDCSDRIVYLVDFSYKKEVVKEICKVAYQVILIDHHKTAVEDLE
                     PLQNAESTEYQKNFEWYVDMDRSGAMLAWDYWNNQAEYGTIAKGDLAYVAPPLLLDYV
                     QDRDLWKFKLTNSREVSAAVFSHKYSFEMWDKLMLEKSVVSMASEGSAIERKHHKDIE
                     ELLNVCQHMGRIGGYNVPVANLPYTLASDAGNKMAAGFEDGRFFAATYYDTGIDRVYS
                     LRSRPNGMDVSQIAASYGGGGHRNAAGFKIPLTQILKESDDGK"
     gene            3572..3841
                     /locus_tag="PKH07_03690"
     CDS             3572..3841
                     /locus_tag="PKH07_03690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34080.1"
                     /translation="MMANESERSKVILIDPNAKTENFKRSNHNDFATSAELKQRKFSG
                     WRMNGITGNAELWIEGNLDMQVLGSEIMRNPTAVEEAHAKRFGLI"
     gene            complement(3898..>4351)
                     /locus_tag="PKH07_03695"
     CDS             complement(3898..>4351)
                     /locus_tag="PKH07_03695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34081.1"
                     /translation="SLSRPIARGAEIGMALTGEGGSINQAGKTVQTSDEVLTTNGIFS
                     RVLASRPLEEAQLRDMQRMNRFYEAADKDRRERTINKLRTAFRNGTNTPSKVQEIAEE
                     YMRNGGSPVGWRTVYRTAQAKTNTPGMDTFIEDIDETNPLNHMIDSLD"
BASE COUNT         1166 a         1018 c         1067 g         1100 t
ORIGIN      
        1 gagttcttat cgagcagttg aggaacgcct ccccctatac cttcaccaat tacaagcgaa
       61 cctttcctct gactttccca gctctttatg ggaacttcga ggggcttaag attgaccaag
      121 agaagcgcac acaacttcgt gaaacagcag agatcaagtt gaagaaagcg cgtcaacgcc
      181 ttcagactat gttcgctgat ccgaatttca atcctggttc ttggcaacag gtggagcatt
      241 atgtctacgg tatcttcggc gccatcaagc ccaaggtagg caagagcaaa tcagggactg
      301 acgagaagaa cctgaaggtt gtcggagaac agcatccctt ccttctcagg atgactgagg
      361 aaatcttgga ataccgtgaa aaccagaagg ccatcggaac ctactatgat ttccttcagc
      421 tcaaggggcg ccttatgtgg gctcttgatc cgtttggaac tgatacaacc cgcatggcct
      481 gcaaggcttc cagcttctgg gtcggcacgc aggttcagaa cgtccccggt tatgcaaagg
      541 aaatgctgat tgcagatgaa ggctttgaga tttttgaggc cgacaacaag caatccgaag
      601 gacgcaccac tgcctattgc tcacaagaag aagccctgat ccttgctttg gaagacgctc
      661 atagggactt ctacaaaacc cttggcactc tgttcttcga cattccttat gaagaagtga
      721 cggacttttt ccgcaacaag gttctgaagc gcatcgttca tggtaccaac tacatgatgg
      781 gagccaagac cttcatcgaa aatatcggtg tgcggattct gctggagacg gcaggaaagc
      841 tgggcattgt cattgtggaa actccacgag ccaataggcc gaatcatcgt actctgcatc
      901 agtttgccaa ggatcttctt gatgcatacc ataagccctt cccacgggtt cgtgaatggt
      961 acaaggagat tgaggaagag atcagggata ctggcttctt ggtttctcct accggccaag
     1021 tgcgtaggtt cttcggggat attcgacgcc agcacacaat gctgcgttct gctgtggctc
     1081 accagcctca acagctttct gttgaaattc tgaacaaggg attcctccgg gcctatcatg
     1141 aaatctgcct tcctagtaat ggggacttcc ggctcaaggc acagattcat gactctatct
     1201 ttggccaatg gagaatctct cgtcgagatg aatttgctca gaagctggca gactgcatgt
     1261 acaaccctac tgtgattcat ggaaggactc ttgtgattcc cattgacatt aagtacggac
     1321 agaactgggc agaggcagat gagagcaatc ccaacggcac tgtgaaatat aagggcttga
     1381 aaaaatgacc agtgatttcg taaccgacta catgaggctt tgcaatctgg gagcctcaga
     1441 agcccctgcg atgtaccatc ggtggtgcgc cttgtctgct atgggcgcat atctcggaag
     1501 acaggtgtat gtgaacttcg gaattggcaa catttatccg aatcaataca tcatgcttat
     1561 gggagcccct ggtactcgca agggaactgc tatgagtatc ggcaaaaagc ttctgaaagc
     1621 ggctggatat acacgcttcg ccaaagataa gacctctaag gaaaggtttt tgatggacat
     1681 gaagcatatt gacgaatcct ctgccatgct ggaagacctg gaagttcttg caatggacac
     1741 tcctgctgag tcctatgtaa tggctcctga atttacagat tggattgggc aaggcaacat
     1801 ggattttgtg atgtttctca cgaacgtctg ggacaatctg gataaatacg agcatccaaa
     1861 gattcatggc aagagtgttg aagtgttcaa gcctactgtg aatttgctgg gctgtaatac
     1921 ggcagaaggt tttgccctgg cctttcctcc agaagctctt ggtacaggct tcttgtctcg
     1981 aacactgatg ctctatgccg aagtcactgg aaagcgtgtg gcctttcctg agccgacaga
     2041 cgaactcctt ttgcacgatc tggccaacag gatgatcgaa gtgcgaaaga atatcaaggg
     2101 agagattgtc ctgaatgagg atggcagagc agcagccaaa gaaatctacg agaacgagat
     2161 tcctgtcgat gatccacgat tcacgcatta ccaacaacgt cgttatattc acatgctgaa
     2221 gattgcgaca ctgctttcag tttacaatag caaggagaaa atcactgaca ttgaaatcgt
     2281 ccgagccaac acggcccttg tggcagccga gaaactaatg cccaaagccc tgggagaatt
     2341 tggagcctct cgatattcga tggtttccgg aaagattcta gcctatctga acacttgcac
     2401 attgcctcag aatagcaatg acctgtggaa ggtggtgagt cgggatctta cgaagatgac
     2461 cgaaatgatc gacattctcc aggggatgca ggtcgcagaa aaaattcaga agatagagat
     2521 cgcaggcaaa agcggatatt tgccgctgcg aaaagcaagt aagacttggc cagactattt
     2581 acttgatctt caatggctta ccgaaactga aaggattatc ttatcatgaa cctcctcaac
     2641 caagacaaca acctcagcct cgtggcattc aagaatcctc ttgtgatcta ccacggaaat
     2701 tgtgcagacg gcttctctgc tgcttgggta ttccaccaca tgcaagataa gatcgaaaca
     2761 gccttcgact ttcatgcagg cgtgtatgga aagcccccac cagattgctc tgatcgcatc
     2821 gtttatcttg tggacttctc ttacaagaaa gaagtggtga aggaaatttg caaggttgcg
     2881 taccaggtga tcttgattga tcatcacaag actgctgtgg aagatttgga gcctttgcaa
     2941 aatgctgaaa gcacggaata ccagaagaac ttcgagtggt atgtggatat ggaccgcagc
     3001 ggagcaatgc tggcctggga ttactggaac aatcaagctg aatacggtac gattgcaaaa
     3061 ggcgatctgg cttatgtagc tccccctctt cttctggact atgtccaaga tcgtgatctc
     3121 tggaaattca agctgacaaa ttctcgtgaa gtctctgccg ctgtgttttc tcataagtac
     3181 agctttgaaa tgtgggacaa gctgatgctg gaaaagagtg tggtctctat ggcttctgaa
     3241 ggaagtgcaa tcgaacgcaa acatcacaaa gacatcgagg agcttctgaa tgtctgtcag
     3301 catatgggtc gtattggcgg ttacaatgtt ccagtggcta acttgccgta cactctggca
     3361 tctgatgcag gaaataaaat ggcagcaggt ttcgaggatg gacgcttttt tgctgcgact
     3421 tactatgaca ctggcattga tcgtgtttat agtctccgtt ctcgtcctaa tgggatggat
     3481 gtatcacaaa ttgcagcttc ttatggggga ggcggtcatc gaaatgcggc aggctttaaa
     3541 atcccgctga cccagattct caaggagtcc gatgatggca aatgaatctg aacgcagcaa
     3601 ggtcattctc attgacccga atgccaagac cgagaatttc aagcgctcca atcacaacga
     3661 cttcgcaact tctgctgaac tgaaacaaag aaaattcagt ggctggagaa tgaatggcat
     3721 tactggaaat gctgaactgt ggatcgaagg aaatctcgat atgcaagtcc tcggtagtga
     3781 gatcatgcga aaccccaccg cagttgaaga agctcacgcc aaacgatttg gcctgatctg
     3841 aaaaaagaaa agcccccttg gattgctcct tgggggcttt tctgtttctg cttcctctta
     3901 atcgagcgag tcgatcatgt ggttcaaggg attcgtttca tcaatgtcct cgatgaatgt
     3961 atccattcca ggcgtattgg ttttggcctg agctgtccga tacactgtac gccatcctac
     4021 aggagaacca ccattgcgca tgtattcttc tgcaatctct tgtaccttgc tcggagtatt
     4081 ggttccattg cgaaacgcag tcctcagctt gttgatcgtc ctttcccttc tatctttgtc
     4141 agcagcctca tagaagcgat tcatgcgctg catatcacgc aattgagctt cttccaaagg
     4201 ccttgatgca agaacacgcg agaagattcc gttcgttgtg agaacttcat ccgaggtctg
     4261 tacagtcttt ccagcctggt taatagaacc accttcacca gtaagagcca taccgatctc
     4321 agccccgcga gcgattggcc tactcaagga c
//
LOCUS       DAOQUC010000144         4345 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_22576_length_4345_cov_2.870862, whole genome shotgun sequence.
ACCESSION   DAOQUC010000144 DAOQUC010000000
VERSION     DAOQUC010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4345
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22576_length_4345_cov_2.870862"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..590)
                     /locus_tag="PKH07_03700"
     CDS             complement(<1..590)
                     /locus_tag="PKH07_03700"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNT34082.1"
                     /translation="MQSNRRDFLRQSALAGLGLAAAGCSTPSQFGFGILKAKRPSFLL
                     FTADDMNYDTPGFAGCRVPDISPNLDALAKQGIYFTQAHVSIAVCQPSRSVLMTGRYP
                     HRNGAMGFEPIREDIPTLIERLKDVGYATGILGKEGHLKPFAKFPWDLVKKGSDLSEG
                     RSPDAYQDTVSKFIRKARSEGKPFFLMVNSHDPHRP"
     gene            complement(649..2139)
                     /locus_tag="PKH07_03705"
     CDS             complement(649..2139)
                     /locus_tag="PKH07_03705"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNT34083.1"
                     /translation="MNRETTRRDFMKHSALAGLGLAATGCNRTQIAKTTSTDRGRPNF
                     VFVMTDDQAWNTMSCAGHPIVKTPNMDRLAAEGVYFENAFVTTSLCSPSRASFLSGVH
                     AHIHGVVGNGIMELPPEISSYPALLQRSGYETAFIGKWHQNTDKNPNPRPGFDYWLSF
                     RGQGVYENPMLNLNGEDFRQEGFMTDILADYAVQWLQQPRSKPFSLCLWHKAVHQPFT
                     AAPRHKGVYANAVLPEPQNFRDEYADKPEWLRRIRTYPPRRKEYLQNLDKPVPERVPQ
                     TEWKGSAPERLAYLESLISVDESLGRVLNTLEGMGQLENTVVVFASDNGYFLGDHRLG
                     DKRLIYEESLRIPLLIRYPKRFKPATKVNGMALNIDIAPTFLELAQVATTARMQGISL
                     VPLMEGSMERVRDSFLYTYFREEWHPGIPLIQCVRTERYKYARYPDVNDIEELYDLEN
                     DPGELRNLAVLPEFNGLKQQMQRRLEELLEKADYPKDAQLGSPPVR"
     gene            2294..3613
                     /locus_tag="PKH07_03710"
     CDS             2294..3613
                     /locus_tag="PKH07_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007919244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HNT34084.1"
                     /translation="MNRPNILYIHSHDTGRCVSPYGYAVDTPNIHALANEGILFRNAF
                     CAAPTCSPSRAGLLSGSSPHSCGILGLAHRGFRMQEDKHLVNFLKKAGYTAHLCGVQH
                     ETDNPRASGYDTIHWRIPRPSVGEGTASAFAEEFLDSKPQQPFFLSVGFTDTHRPYPA
                     LEPQDDTKYIQVPSCVPDLPETRSDWAAYRKSVKNLDNGMGVVLSALKRSGLMDSTII
                     LCTTDHGIAFPRMKCNLTDAGMGVMLIVRWPERIQPGIVCDALVSQIDVFPTLCELLS
                     LEPPNWLEGKSLLPLLDDPQHEINDEVFGEVTFHASYEPQRSIRTTRYKYIRRFGSRT
                     ELTRPNIDASPTKSVLLEAGFGRFEQVREQLYDLRLDPHEFRNLADDTGHKSVLDDLR
                     TRLQDWMTRTNDPLLQGEVPLPAGARITDPNEPEPEGRMIVNEGNKP"
     gene            complement(3654..>4345)
                     /locus_tag="PKH07_03715"
     CDS             complement(3654..>4345)
                     /locus_tag="PKH07_03715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34085.1"
                     /translation="GEDGLSTAGAMRHAIETLSAKPCSIFLWRKVLLFTRERLVAACT
                     SLEMGETAGTESLVGLGEREILVLDQTTALGASACIPGALRAYDLPGNSPRNTRRERK
                     TTKRTMIRGKIARHAAIHLPSLRLVHAILPTNVLSTEITRYRIGGNGKKKQTEEKQKE
                     SDRKKRSKEQVASAFTQVNPSSVGAFKSLHWIQNEFRKSSVTVGWQTLGTVAVQYLSL
                     PPRLLPLSCTQ"
BASE COUNT          949 a         1177 c         1149 g         1070 t
ORIGIN      
        1 aaaggccgat gcggatcatg cgagttcacc atgagaaaga agggcttgcc ctcggatctg
       61 gcctttctga tgaacttgct gactgtgtcc tgataggcgt ctggacttcg cccctccgac
      121 agatcggaac ctttcttgac gagatcccaa gggaatttgg caaaaggctt caggtggcct
      181 tccttgccca gtattcccgt tgcgtagccc acatccttca gacgttcgat cagcgttggg
      241 atatcctcgc ggatcggctc gaagcccatc gcgccgttgc ggtgtggata gcggcctgtc
      301 atcagaacgg aacgactggg ctggcagact gcgatgctaa catgggcttg ggtgaagtag
      361 attccctgtt ttgccagagc gtccagattc ggggaaatgt caggaacacg acaaccggca
      421 aaaccggggg tgtcgtagtt catgtcgtct gctgtgaaca aaaggaaact cgggcgtttg
      481 gccttcagaa tgccaaaccc gaactgcgac ggtgttgaac agcctgccgc cgccagtcca
      541 agacctgcca atgcgctttg tctcagaaaa tcccgtcggt tgctttgcat cgtcactcca
      601 gttctcttgt gctcggtgcg cttcattccc gtgagagaag aacagattct atcggacagg
      661 tggagaaccc aactgggcat ccttggggta atcggccttt tcgaggagtt cttccagccg
      721 tctctgcatc tgctgcttca gaccgttgaa ctcgggcaat acggcgagat tcctcaattc
      781 cccagggtcg ttctccaagt catagagttc ttcgatgtcg ttcacatccg gataccgtgc
      841 atatttgtat cgttctgtcc gaacacattg gatcaagggt attccgggat gccattcctc
      901 tcggaagtaa gtgtaaagaa aagagtctct caccctctcc atgctccctt ccatgagggg
      961 aaccagggag attccctgca tccgggctgt tgtcgccact tgagccagtt ccagaaacgt
     1021 tggtgcgatg tcaatgttga gtgccatgcc gttcaccttc gttgcaggct tgaaccgttt
     1081 cggatatctg atcagaagag ggattctcag tgactcctca tagatcagcc gcttgtctcc
     1141 aaggcgatga tctccgagga agtaaccgtt gtcactggca aacaccacaa ccgtgttctc
     1201 caactgaccc attccctcaa gcgtgttcag aacacgcccc aggctctcat ctacggaaat
     1261 cagtgattcg agatacgcaa ggcgctcagg agcgcttcct ttccactcgg tctgtggaac
     1321 tctttcagga acgggcttgt caaggttctg cagatactcc ttgcgtcgtg gcggataggt
     1381 acggattctt cgtaaccatt ctggcttgtc ggcatactca tcacggaagt tctgaggttc
     1441 gggcagaact gcgttcgcgt agactccctt gtggcgtggc gctgccgtga agggctgatg
     1501 cactgccttg tgccacaggc acagggaaaa cggtttggaa cgaggctgct gcagccattg
     1561 aaccgcgtag tccgccagaa tatccgtcat gaaaccttcc tgtcgaaagt cctcgccatt
     1621 caggttgagc atggggttct catagacgcc ttggccgcga aaactcaacc agtaatcaaa
     1681 tcctggccga gggtttggat tcttatcggt attctgatgc cactttccga tgaacgctgt
     1741 ctcatatccc gatctctgca gaagtgcagg ataactcgaa atctcgggag ggagttccat
     1801 tatcccattg cccacaacgc catggatgtg agcgtgaaca ccactcagga aactggctcg
     1861 acttggagaa cagagcgatg tggtcacaaa ggcgttctcg aagtagacac cttctgcagc
     1921 cagacgatcc atgtttggtg tcttcacgat cggatgcccg gcacaactca tcgtattcca
     1981 tgcctggtcg tctgtcatca caaagacaaa gtttggccgt cctctgtctg tgctggtggt
     2041 cttcgcgatc tgcgttcggt tgcatccggt ggcagccagg ccgagaccag ccagtgcact
     2101 gtgtttcata aagtcccttc gtgtcgtttc tctgttcatt tgtccacctt cgagcaggcc
     2161 agaggctctg aatatgagag ccgcacccaa tgtccttgag atctatccga tgcgataccc
     2221 aatagattcg actttatgac cgactgtttg actttgtcaa ggcagtgtgg aattctcttt
     2281 tcggagggac aaaatgaaca ggccaaacat tctctacatt cactctcacg atacagggcg
     2341 atgcgtcagc ccgtatggtt atgctgtgga caccccaaac atccatgctc ttgcgaatga
     2401 gggtatcctg tttcgcaatg cgttctgtgc ggcccccacc tgttctccaa gccgtgcagg
     2461 tttgctgagt ggttcgtcac cgcacagttg tggcatcctc ggcctggcgc atcggggttt
     2521 tcgtatgcag gaagacaagc atctggtcaa ctttctcaag aaagccggct atacggcgca
     2581 tctctgcggt gttcagcatg agacggacaa tccaagggcg agtggctacg atacgattca
     2641 ttggcggatt ccacggccgt cagtcggtga gggtactgcc agcgctttcg ctgaggagtt
     2701 cctcgatagc aagcctcagc agcccttctt tctttcagtc ggttttacgg atacgcatcg
     2761 tccgtatcct gcactcgaac cccaagacga caccaagtat atccaggtgc cctcttgtgt
     2821 tcctgatctc ccagaaactc gatcagactg ggctgcctat cgcaaatccg tcaagaacct
     2881 cgacaacggc atgggcgtcg tattgagtgc tctcaagcgc agtggtttga tggacagcac
     2941 aatcattctg tgtacaacag atcacggtat cgcctttccg cgtatgaaat gcaaccttac
     3001 cgatgcaggc atgggggtca tgctcattgt ccgatggcct gagcggattc agcccggcat
     3061 cgtgtgcgac gctctggtct cacagatcga tgtctttcca acactgtgcg aactactgag
     3121 tctagaacca cccaactggt tggaagggaa gtctctgctt ccattgctgg acgatccgca
     3181 gcacgagatc aacgacgagg tttttggcga ggtgaccttc catgcgtctt acgagcctca
     3241 gcgatccatc cgcacaacgc gttacaagta catcaggcgt ttcgggtcgc gcacagaatt
     3301 gactcgacca aacattgacg cgagtccgac aaagtccgtt ctgcttgaag caggattcgg
     3361 cagattcgaa caggtacgag aacaactata tgaccttcgg ctcgatccgc atgagttcag
     3421 aaacctcgcg gacgacacag gtcacaaatc ggtcttggat gacttacgca ccagactcca
     3481 ggattggatg acccgcacca acgaccctct tctgcaaggc gaagtgccat tgcctgctgg
     3541 agcgaggatc acggatccaa acgagccgga gcctgaagga aggatgattg tcaatgaggg
     3601 gaacaagcct tagcatccac gcggggctca gtcgagtcat ctttgttctt gatctattgg
     3661 gtgcaagaga gagggagcag gcggggggga agagacagat attgcacagc aacagtccca
     3721 agagtctgcc acccaactgt tactgaggac tttctgaatt cgttttgtat ccaatggagt
     3781 gatttgaacg ctccgactga cgagggattg acttgagtaa atgcagaagc aacttgttcc
     3841 ttcgatcgct ttttgcgatc cgactctttt tgcttttctt cggtctgctt tttcttgccg
     3901 tttccgccta tgcggtaacg tgtgatctct gtggagagaa cattcgtggg cagtatcgcg
     3961 tgtaccagac gcagcgaggg cagatgaatt gctgcatggc gtgcgatctt tcctctgatc
     4021 attgtgcgct ttgtcgtctt cctctcgcgt cgggtgtttc gcggcgaatt gccggggaga
     4081 tcgtatgccc tcagtgcgcc cggaatgcaa gcacttgcgc cgagtgcggt tgtctgatcc
     4141 agaacaagta tttcacgctc tccgagacca accagacttt ctgtgccagc tgtttctccc
     4201 atctccagcg atgtgcacgc tgcaacaagc ctctctcggg tgaacaaaag aaccttccgc
     4261 cacagaaaga tggaacaagg ctttgccgac agtgtctcga tagcgtggcg cattgcgccg
     4321 gctgtggaaa gaccatcctc tccac
//
LOCUS       DAOQUC010000145         4323 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_22738_length_4323_cov_2.975164, whole genome shotgun sequence.
ACCESSION   DAOQUC010000145 DAOQUC010000000
VERSION     DAOQUC010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4323
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22738_length_4323_cov_2.975164"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..794
                     /locus_tag="PKH07_03720"
     CDS             <1..794
                     /locus_tag="PKH07_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNT34086.1"
                     /translation="DTLTAVFNDLDSVQRIVGSLLDQIAAIILEPVAGNMGVVPPQSG
                     FLQGLRELCTESGIVLIFDEVMSGFRVAPGGAQALYGVRPDLTTLGKIIGGGLPVGAY
                     GGRAEIMDCVSPLGPVYQAGTLSGNPLAATAGYVTLQRLLENGFYDRLEQMSATLEQG
                     LLNAAREAAVSITINRVGSMLTVFFTDQPVTDYESALKSDTTRFATFFRSMLKQGVFL
                     PPSQFEALFVSSAHSEADVQATVAAAIKAFSTTTAAADSCPPDRH"
     gene            complement(751..2124)
                     /locus_tag="PKH07_03725"
     CDS             complement(751..2124)
                     /locus_tag="PKH07_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018047172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HNT34087.1"
                     /translation="MEFEKRILCIGAGYVGGPTMAVIADKCPQYRVELVDINEERIRR
                     WNSEDLPIYEPGLDEIVRRARGRNLFFTTEVATAIEHSDIIFVSVNTPTKAFGQGAGQ
                     AADLQYWEKTAYQILKHSHSDKIVVEKSTLPVRTAEAMQRILHSRGTSPRFDVLSNPE
                     FLAEGTAVENLLQPDRVLVGCMNTESGHKACDQLEAIYANWVDRSRIIRTNIWSAELS
                     KLVANAFLAQRISAINSISALCEKTEADVDEVAHSIGLDSRIGKKFLKAGIGFGGSCF
                     KKDILNLVYICRQHGLDEVADFWERVVEINEYQQSRFVANMISEMFNTIAGKSILVLG
                     FAFKADTGDTRESPAAQVCRRLLEEHAQLRLCDPKALENARKELIGVQGVSFHEDPYE
                     AAENVHAVALLTDWQCYRELDYAKVFDRMEKPAFLFDGRNLLDHRRLFEIGFNVYPVG
                     KSPLRQL"
     gene            complement(2192..2818)
                     /locus_tag="PKH07_03730"
     CDS             complement(2192..2818)
                     /locus_tag="PKH07_03730"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HNT34088.1"
                     /translation="MDQTNNISLDHCKTEIILASQSPRRQEFLRDWGICFECMPSGIE
                     EPEREAGEPLGQYVERISLLKAKHVAPNHPDRLILGADTVVALGETILGKPVDDTQAA
                     EFLRMLSGKKHHVCTGVAFYCHREGWFHVDHVVTDVHFRSLNEEEIEDYVATGEPRDK
                     AGAYAIQGRAFDFVIQVEGAWDNVVGLPREAVMRGMNAFDGRPSGCRP"
     gene            complement(2919..>4323)
                     /locus_tag="PKH07_03735"
     CDS             complement(2919..>4323)
                     /locus_tag="PKH07_03735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34089.1"
                     /translation="SGVAAFVAGIGGEERIQQGRMTLLDHVVAPFDGTEPIARYLLWP
                     FDGPVEIGFLRLRRFIGTEYSCQRDKYSTFSWNQFWLWQGGIEPAKSEVVTAERLCEI
                     LPRIKAIGCEEFHLDAGWEITPGDWRFDPVRFPEGFEPIRRLLREKGMRYHTWMNTAA
                     SEDPAVITSLVEETDMCKLFQDRTVDEKAVAAMREVRKRYPDLETFVHHSTSRSKCYW
                     WGNLHFLSDLNQIYFGEGEFWAWSNILPEKPEGDANTRFFSRHSLRAGDLVTRSAAYQ
                     VHWAWPYMCIVPPHCGWAWFEDRDLSELASRMFTTIAARADYQWGEDPRMLRPEVLNF
                     FLEWTAFFKTAKPYLSQYQHVLAPPDGIHPDGAAHLLDGRGFIVLCNPSQSRASVSLK
                     DLLWEPELELHPKEPVHLTDWTAPLAPVNLQKVNLLKPKGSIVMQPLSYRVLGVNVDK
                     DSVLGEVRRQRAALHAQ"
BASE COUNT          968 a         1233 c         1100 g         1022 t
ORIGIN      
        1 ctgacacgtt gactgctgtc ttcaatgatc tggattcggt acagcgtatt gtcggctcgt
       61 tgctcgacca aatcgcagcg atcatccttg agccggttgc cggcaatatg ggcgtcgtac
      121 cacctcagtc tggattcctg caaggacttc gagaactgtg tacggaaagt ggcattgtcc
      181 tgatattcga tgaggtgatg agcggctttc gggtggctcc aggtggggcg caggcacttt
      241 acggtgtccg accagacttg actaccttgg gcaagatcat tggtggaggc ctgcctgtcg
      301 gtgcctatgg tggacgagca gaaatcatgg actgtgtcag tccgcttggg cctgtgtatc
      361 aggctgggac actgtcgggg aatcccttgg cggcaacggc tggttatgtc actttgcaga
      421 ggcttctcga aaacggcttc tacgaccgtc tggagcagat gtccgcaaca ttggagcagg
      481 ggctgctcaa cgcagcgcgc gaagcagcgg tttcgatcac aatcaaccga gtcggttcca
      541 tgttgactgt cttcttcact gatcaacccg tcacggacta tgagtcggca ctcaaatcag
      601 acaccacacg gtttgccacg ttctttcgat cgatgctcaa acagggagtc tttcttcccc
      661 cgtcacagtt tgaagcactt tttgtttcct cggcacactc tgaagcggat gttcaggcaa
      721 cagtggctgc tgccatcaag gccttttcca ctacaactgc cgcagcggac tcttgcccac
      781 cggatagaca ttgaatccga tttcaaacaa ccgcctgtga tccagcagat tgcgtccatc
      841 gaaaaggaat gcgggcttct ccattcgatc aaacaccttg gcgtaatcca gttcacgata
      901 gcattgccag tcagtgagaa gcgccactgc gtgaacattc tcggctgcct cgtatggatc
      961 ttcatgaaaa gaaacgcctt gaacgccgat gagttccttt cgggcgtttt ccagagcctt
     1021 aggatcacaa agccgcaatt gagcgtgttc ttccaagagt cttcggcaaa cctgtgcggc
     1081 aggagattca cgggtgtcgc ccgtgtctgc cttgaatgca aatccaagca cgaggatgct
     1141 ctttccggcg atggtgttaa acatctctga aatcatgttg gcgacaaagc gactttgttg
     1201 gtactcattg atctcgacca cgcgctccca gaaatcggca acctcgtcca acccatgctg
     1261 gcgacagatg tatacgagat tgagaatgtc tttcttgaaa caggagccgc caaatccaat
     1321 gcctgccttg agaaacttct taccgatgcg cgaatccaga ccgatggagt gcgccacttc
     1381 atccacgtcc gcctcggttt tctcgcagag tgcagaaatg gagttgatgg ccgagatgcg
     1441 ttgcgccaag aatgcattgg ccaccagctt ggacaactct gcgctccaga tgttggttcg
     1501 gatgatccgg gatctgtcta cccagttggc atagatcgct tcaagttggt cacaggcttt
     1561 gtgaccagat tctgtgttca tgcagccaac gagaacccga tcaggttgca ggagattttc
     1621 gaccgccgtt ccctcggcaa ggaattcagg gttcgacaag acgtcaaagc gcggcgacgt
     1681 cccgcgagaa tgcagaattc tctgcatcgc ctcggcagtt cttacaggca gtgtgctctt
     1741 ctcgacgacg atcttgtccg agtgggagtg tttgagaatc tgatacgccg tcttctccca
     1801 atactgaaga tccgcggcct gtccagcgcc ttgcccaaag gctttggtcg gggtgttgac
     1861 actgacgaag attatatcgg aatgctcaat cgctgttgca acttctgttg taaaaaagag
     1921 atttcgcccc ctggcgcgtc tgacaatctc atccagaccg ggttcgtaga tgggcaaatc
     1981 ctccgaattc catcttcgga ttctttcctc attgatgtct accagttcga cacggtattg
     2041 agggcatttg tcggcaatga ctgccatcgt tggccctccg acataacccg caccaatgca
     2101 gagaatgcgc ttttcaaatt ccatcttcag tctcctatcg ccttcattca ggcaacatat
     2161 tcctgaccgc gccgtgcctt cgacctctgg atcatggacg acacccagat ggccggccgt
     2221 caaaggcatt catccctcgc atgacggctt ctctcggaag tccgacgacg ttgtcccaag
     2281 ccccttcaac ctgtatcaca aaatcaaagg cgcgaccttg aatggcatac gcaccggctt
     2341 tatcccgtgg ctcaccggtt gcgacatagt cctcgatctc ctcttcgttg agcgaccgga
     2401 aatgtacgtc ggtgaccaca tggtcaacat ggaaccaccc ttcacgatga caatagaacg
     2461 caactcccgt acacacatga tgcttcttcc cagagagcat acgcagaaac tccgcagcct
     2521 gtgtgtcgtc aacaggtttg cccaggatcg tctctcccag ggcaaccact gtgtctgcac
     2581 ccaggatcag acgatcgggg tggttgggcg cgacatgctt ggctttcaag agcgaaatcc
     2641 gctcgacata ttgccccaga ggctcccccg cttcgcgttc gggttcctca atgccagaag
     2701 gcatacactc aaagcagatc ccccagtctc gaaggaattc ctgacgtctg ggcgattggc
     2761 tcgcgagaat gatctctgtc ttgcagtgat ccaacgaaat gttgtttgtt tgatccatct
     2821 ctcgatatcc ctgccatcgg gcagttcatt gactccttct accttagcac gtcagatcac
     2881 atttggggag tgtccgctga gccagtgccc atgatccttc attgagcgtg caatgctgct
     2941 ctttgacgtc ttacctcgcc aagcaccgaa tccttgtcaa cgttgactcc caacactctg
     3001 taactcaacg gttgcatgac gatggatccc tttggcttca agaggttgac cttctgcaaa
     3061 ttcacaggcg caagaggtgc tgtccagtca gtgaggtgga caggctcctt aggatgcaac
     3121 tccaactcag gctcccagag caagtccttc agcgatacgc ttgccctgct ctggcttggg
     3181 ttgcacagca cgatgaaacc ccggccatcc agaagatgcg ctgcaccgtc tggatgaatg
     3241 ccgtccggtg gtgcgagcac atgctgatat tgcgacagat agggcttcgc tgtcttgaag
     3301 aatgcagtcc actccagaaa gaagttgagc acctcagggc gcaacatgcg cggatcctca
     3361 ccccactggt agtcagcgcg agccgcgatt gtcgtgaaca tcctgcttgc aagttctgac
     3421 agatcgcgat cttcaaacca agcccatcca cagtgtggcg gcacaatgca catatacggc
     3481 caagcccaat gcacctgata cgcggcagag cgtgtcacca ggtctcctgc acgaaggctg
     3541 tgacggctga agaagcgcgt gtttgcatca ccttctggtt tctcaggaag gatattcgac
     3601 cacgcccaga attcgccctc gccgaaatag atctgattca aatcgctcag aaaatgaagg
     3661 ttgccccacc aatagcattt gctgcgggaa gtggaatggt ggacaaaagt ctcaaggtcg
     3721 ggatatcgtt tgcgcacttc ccgcattgcc gctacggctt tctcatccac tgttctgtcc
     3781 tgaaacagtt tgcacatgtc cgtttcttca accaaagaag tgatgacagc gggatcttca
     3841 gaagcggcgg tattcatcca ggtgtggtat ctcatgccct tttcacgcag cagtcgtcga
     3901 atcggctcaa acccctcagg gaagcgcact ggatcgaaac gccaatcgcc aggagttatc
     3961 tcccaaccgg catcgaggtg aaactcctca catccgatgg ctttaatgcg aggaagtatc
     4021 tcacacagcc gttccgctgt tacgacttcg ctcttggccg gctctattcc gccctgccac
     4081 agccagaact ggttccaact gaatgtcgaa tacttgtccc gctgacagga atactcggtg
     4141 ccgatgaaac gcctcaagcg aaggaaaccg atctcgacgg gtccgtcgaa aggccatagc
     4201 aggtatcgag cgatcggttc ggtgccatcg aatggtgcga cgacgtggtc gagcaaggtc
     4261 atccgcccct gctgaatacg ctcctcaccg ccaatgccgg caacgaaggc tgccacaccg
     4321 ctc
//
LOCUS       DAOQUC010000146         4311 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_22822_length_4311_cov_3.334821, whole genome shotgun sequence.
ACCESSION   DAOQUC010000146 DAOQUC010000000
VERSION     DAOQUC010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4311
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22822_length_4311_cov_3.334821"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(96..2450)
                     /locus_tag="PKH07_03740"
     CDS             complement(96..2450)
                     /locus_tag="PKH07_03740"
                     /inference="COORDINATES: protein motif:HMM:NF016071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HNT34090.1"
                     /translation="MRSCSVWSIYTRFAERLLFAAFLLSCCGAWAQQGQWQPHIGYVY
                     PAGAQQGSVTHVIAGGQYLRGANLAPIVGDGVRASVVGYIPHYKKPLNREEMRQLVER
                     LADLKQERSNETETPLEPSLSLQRRSRSRIASTTDTVKPDHPLLRDVELMNLRELEYV
                     SGQIGKSRDKQQRNEQIAEIVLLDVTVDDLASPGEREIRLVSPAGLSNPLRFHVGQLP
                     EVSENEPNDTKASEGTVAQTPVMFNGQILPGDVDRFRLRAKQGDSLVIEAYARRLIPY
                     LADAVPGWFQAVLALYDSSGSRVAFADDFRFDPDPVILYKVPKDGEYELEIRDAIYRG
                     REDFIYRISVSAQPFITSIYPLGARSGEPANVLLTGWNLPVSEAQLETQPGASSLREI
                     RWNWNGLYSNPVAYAVDTLAECEESEPNDTRKESRFLTLPTFVNGCISRRGDVDTFRF
                     VGHVGDRIVAEVNARKLHSPLDSVLRLTDKTGKVIASNDDREDNRIGLQTHQADSYLS
                     IALPEEGTYYLEIADTQKQGGKEYGYRLRVSSPRPDFELMATPSSLFAPAACSVPVTV
                     HALRTDGFDGEIEVVLKDTGHGFKLSGGLIPPDKTSVRITLTAPENAPAEPVSLILEG
                     RATPGGQMVTRPVVAADDLMQAFAYQHLVPAQRLMALVKSIRWRVLPVSLVSALPIQI
                     PEGGTASVRLSVPRRSMARRFQCEIKDPSKGVTLDDVSYSPGVVTLTLKADRELAKSG
                     IADNLIVEVFTEWAGNNQEGKPTQEPRKVSLGVLPAIPFEIVSR"
     gene            complement(2437..3771)
                     /locus_tag="PKH07_03745"
     CDS             complement(2437..3771)
                     /locus_tag="PKH07_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013042686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HNT34091.1"
                     /translation="MVFTENINRRDAIKCALLGSAGLALWDLREVWADVTVPPATVPQ
                     GKATAKSVIQIWMWGGPSHLDTFDPKPDAGNDYCGSLKNPIPTNVKGIQIGELLPLLA
                     QQADKYSIIRSMTHGVNAHETASYMVQTGHSSEDRLVYPCLGAIVSLIKGFDGGYKGL
                     VPPYIVLTEPQGRFSEAGFLGPRYKPFATGGDPSRNPFAVEGIVTEGLSEERRQGRRT
                     LLKNLDTLGKVMPGTAPFDFFDECGHAADSIVAEAGKLFDLSQEKMELRERYGMSKFG
                     QSCLMARRLVEQGVPYITINYNGWDTHKEHFEIMRRKLPEMDKGMSTLLQDLSDRELL
                     DSTVVWWSGEFGRTPRIQWESPWNGGRGHYGKCFSAVLAGGGFKGGQVVGASDAKGEE
                     VAERPVRPEDLLGSILALMGIDPQGKLPNAQGLDLQVMPSSQGTKGLLHEIM"
     gene            complement(3781..>4311)
                     /locus_tag="PKH07_03750"
     CDS             complement(3781..>4311)
                     /locus_tag="PKH07_03750"
                     /inference="COORDINATES: protein motif:HMM:NF019223.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1553 domain-containing protein"
                     /protein_id="HNT34092.1"
                     /translation="SPEGESLFAYYIVRRLDAEVLIDAICQFTQTTESYSSPIPEPFT
                     YVPENQRSITLADGSITSPFLEMFGRPPRDTGLESERGTQVSSAQRLHLLNSTHIQRK
                     IENADWLRDIPRSRRNARGEVVETVYLSILSRYPTSEELDIIRNYVDSSGLWPRLVLN
                     DLAWALINTKEFLYRH"
BASE COUNT          951 a         1248 c         1130 g          982 t
ORIGIN      
        1 gtcgtctccc gtccaagggt gagctggagc attgccccac aagcgatcag atttcttctc
       61 ctctgcgccc gtggaacgga ctggaaccgg tgggtctacc tgctcacgat ctcaaagggg
      121 atggcgggta aaacgccgag ggacaccttt ctcggctctt gggtgggctt gccttcctgg
      181 ttgttgccag cccactctgt gaaaacctca acgatgagat tatccgcaat gcccgacttc
      241 gcgagttcgc gatccgcttt cagggtcagc gtcacgacgc ccggtgaata actcacatcg
      301 tcaagcgtca cccctttgga cggatccttt atctcgcact gaaaccttct tgccatggag
      361 cgcctcggaa cactcagtcg taccgaagcc gtcccgccct caggaatctg aatcggcagg
      421 gcagagacca gggacacagg caatacccgc caccgaatgg acttgaccaa cgccatcaga
      481 cgctgagccg gaaccagatg ctgatacgca aaggcctgca tcagatcatc ggcagccacc
      541 acgggtcggg tgaccatctg accgcccggt gttgctcgac cttccagaat gagtgacacg
      601 ggctcggcgg gtgcgttctc gggagcagtc agcgttatgc gaacgcttgt cttgtcaggt
      661 gggatgagcc ccccactcag tttgaaccca tgacctgtat ctttcagaac gacctctatc
      721 tctccatcga atccatccgt cctcaaagcg tgaaccgtga ccggaacgct gcacgccgcc
      781 ggggcgaaca gacttgacgg agtggccatg agttcgaaat cgggtcgcgg cgagctgact
      841 cggagacggt agccgtactc ctttcccccc tgtttctgag tgtcggctat ctcaagatag
      901 taggttccct cttcgggcaa ggcaatcgag agataggagt ccgcctgatg tgtctgcagg
      961 ccgatccgat tgtcttcgcg atcgtcattg gatgcgatga ccttgcctgt tttgtcagtc
     1021 aaacgcagaa cggagtccaa gggtgagtgg agttttcggg cgtttacctc agcgacgatc
     1081 cggtctccga cgtgtccaac aaaacggaat gtgtccacat cgccacgcct gctgatacat
     1141 ccgttgacga atgtcggcag cgtaagaaac cgggactctt tgcgggtgtc attcggttca
     1201 ctttcctcgc actcggcaag cgtgtccacc gcataggcca cagggtttga atacagtccg
     1261 ttccagttcc atcgaatctc ccgaagtgac gacgcacccg gctgtgtctc cagttgcgcc
     1321 tctgacaccg gtagattcca gcctgtcagc aagacatttg ccggttctcc tgatcgcgct
     1381 cccaagggat agatcgaggt gatgaagggc tgcgcactga cagagatacg gtagatgaaa
     1441 tcctctcttc ctctgtagat tgcatcccgg atctccagtt catattcgcc atccttgggt
     1501 accttgtaga gaatgactgg atcaggatca aaacggaagt cgtctgcgaa ggcgaccctg
     1561 ctgccactgg agtcatacaa cgccagtacg gcctgaaacc aaccgggaac agcatccgca
     1621 agatatggaa tgagtcttcg agcgtatgct tcgatgacga ggctgtcccc ctgtttggca
     1681 cgcaaccgaa aacgatccac atctcctggg agaatctggc cgttgaacat gacaggcgtt
     1741 tgcgccacag tcccctcgga tgcttttgta tcattgggtt cgttctcgga aacctctggt
     1801 agttgtccga catgaaaacg cagtgggttg gacagtccgg caggagagac cagtcgaatc
     1861 tcacgctcgc caggacttgc caagtcatct acggtcacat caagcagaac aatctccgcg
     1921 atctgctcat ttctttgctg tttgtctctg gacttgccga tctgcccgga tacgtattcg
     1981 agctcgcgaa gattcatcag ctccacatcg cgcagcagag gatgatcggg tttcaccgta
     2041 tcagtcgtcg aggcaattct ggaacgagat cttctttgaa gcgaaagcga cggctccaag
     2101 ggtgtttccg tctcatttga gcgttcctgt ttcagatctg caagtcgctc caccaactgt
     2161 cgcatctctt cgcggttcag tggtttcttg taatggggaa tgtagccgac aacagaggct
     2221 cgaaccccgt cgccgacgat gggagccaga ttcgcacctc gaagatactg gccacccgca
     2281 ataacatgag ttacagagcc ctgctgcgct ccagccggat acacgtaacc aatatgaggc
     2341 tgccattgcc cctgttgtgc ccaggcgccg cagcagctga gcaggaaagc agcgaaaagg
     2401 agtcgctcag caaatcttgt gtaaatactc caaacactac atgatctcat gaagtaatcc
     2461 tttggttcct tgtgacgatg gcatcacttg aagatccaaa ccttgtgcat ttggcaactt
     2521 tccctgagga tctatgccca tcagcgccag gatgcttccg agcagatcct ccggtctcac
     2581 cggtcgctct gccacttctt cccctttggc gtcagaggca ccgacgacct ggcctccctt
     2641 gaagcctcct cccgcaagca cagcggagaa gcacttgccg tagtggccac gtcccccatt
     2701 ccagggtgat tcccactgaa tcctcggggt gcgtccgaac tcgccactcc accacacgac
     2761 agtgctgtcc aaaagctccc gatcggagag atcttgcagc agcgtggaca tgcccttgtc
     2821 catctcagga agcttccttc gcatgatctc gaagtgttcc ttgtgcgtgt cccaaccgtt
     2881 gtagttgatc gtgatatagg gcaccccctg ttcgaccagg cgacgcgcca tcagacagga
     2941 ctgaccaaat ttgctcatcc cgtagcgttc gcggagttcc atcttctctt gagagagatc
     3001 aaagagtttc cccgcttcgg caacgatgct gtccgctgca tggccacact catcgaagaa
     3061 atcgaacgga gcagttccag gcatgacctt gccgagagtg tcgagattct tcaaaagagt
     3121 ccgacgtccc tggcgtcgct cttccgagag accttccgtg acaatgcctt ccacagcaaa
     3181 cgggttacga ctgggatcac cgcccgtcgc aaagggcttg taccgcggtc cgagaaagcc
     3241 tgcttcggaa aaccgtcctt gaggttccgt gagcacaata tacggtggta ccagaccttt
     3301 gtatccccca tcaaatccct tgatcagcga cacaatagct cccaggcagg gatagaccaa
     3361 tcggtcttca gaactatgac cggtctgaac catgtaagat gccgtctcgt gggcattcac
     3421 tccatgggtc atacttcgga tgattgaata cttgtccgcc tgttgtgcaa gaagtggcag
     3481 caactcgccg atttgaatgc ccttgacgtt ggtgggaatc ggattcttga gagagccaca
     3541 atagtcgttt cccgcatccg gcttgggatc gaatgtatcc agatgcgaag gcccgcccca
     3601 catccaaatc tggatcacgg atttcgctgt tgcctttcct tggggaactg tcgcaggtgg
     3661 cacggtaacg tccgcccaca cctctctgag atcccaaaga gccaggccgg cagagccgag
     3721 cagagcgcac tttatcgcgt cacgtcggtt gatgttctct gtaaagacca tacggaatcc
     3781 tcaatgacga tagagaaact ctttcgtgtt aatgagcgcc caagccagat cgttgagcac
     3841 cagtcgaggc cacaggccgg aactgtccac gtagtttctg attatgtcca actcttcgga
     3901 cgttggatac cgcgacagaa tactcagata gaccgtttcg accacttcac ctcgtgcatt
     3961 tcttcgggaa cgagggatgt cacgcagcca gtcagcattc tcgatcttgc gttgaatgtg
     4021 tgttgagttc agcaggtgca aacgctgggc gctcgacact tgtgttccgc gttccgattc
     4081 aagacccgtg tcgcggggag gccttccgaa catctcaaga aaaggacttg tgatactccc
     4141 atctgccagt gtgatggaac gctggttctc gggcacatag gtaaatggct caggaatcgg
     4201 actggaatag ctctcggtcg tttgggtgaa ctgacagatg gcatcaatga gcacttccgc
     4261 atcgagtcgt cgaacgatgt aataagcaaa cagcgattcc ccctctggac t
//
LOCUS       DAOQUC010000147         4301 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_22894_length_4301_cov_3.430523, whole genome shotgun sequence.
ACCESSION   DAOQUC010000147 DAOQUC010000000
VERSION     DAOQUC010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4301
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22894_length_4301_cov_3.430523"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="PKH07_03755"
     CDS             <1..345
                     /locus_tag="PKH07_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010526902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5109 domain-containing protein"
                     /protein_id="HNT34093.1"
                     /translation="NEIMSGITGSVDAVAFQDGHVDFAQLPEFLEINSHLARKHGLHC
                     WTNSETFDRDMPIKFLPIKWEKLLLKLRAAEKAGVEKAITFEFSHFMSPNSCYCQAHG
                     LYRRYCEHFGIV"
     gene            357..1550
                     /locus_tag="PKH07_03760"
     CDS             357..1550
                     /locus_tag="PKH07_03760"
                     /EC_number="5.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015143408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HNT34094.1"
                     /translation="MDREQFAKFETLYQDAFLNDVIPFWMKHSLDREQGGYFTCLDRA
                     GTVYDQDKFMWLQCRQVWTFSMLYNRLDRRSEWLDVASLGFEFLRKYGRDAEGNWYFA
                     LDRLGRPLVQPYSIFSDCFAAMGFSQYALASGNEEAKEIALATYRNILKRKNNPKGKY
                     SKVVPETRPMKDFALPMILCNLGLEMQWQLDSTDLDRTIDICIDEVMNQFRDKDRGII
                     FENIAPDGSRLDCFNGRLICPGHGMEAMWFIMDIGRKRSDRQLIDQAVDTMLSVLDFG
                     WDRKHGGIFYFMDSEGRPPRQLEWDQKLWWVHLESLVALVMGYALTERTECWTWYEKL
                     HDYTWNRFPDPDFGEWFGYLNRQGDVLLPLKGGQWKGCFHVPRALYLCWQEFKKLREG
                     RPQTK"
     gene            1756..2604
                     /locus_tag="PKH07_03765"
                     /pseudo
     CDS             1756..2604
                     /locus_tag="PKH07_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014830310.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
     gene            2776..3042
                     /locus_tag="PKH07_03770"
     CDS             2776..3042
                     /locus_tag="PKH07_03770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34095.1"
                     /translation="MMTIPKDLEERWRQVVAKSFNDDQEAALREALLRFITEEEKRIP
                     KSGQFDSVLSKVQHRQSNLDDILADGLSDALDRMKKRKERGLPL"
     gene            3195..>4301
                     /locus_tag="PKH07_03775"
     CDS             3195..>4301
                     /locus_tag="PKH07_03775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNT34096.1"
                     /translation="MKDKPGKRKTPAKPSDAPKADSRGRRAKDKNTVCSPRKLASSPS
                     NSVAVEISRLVLVGSELAAIEDLMDLCRRAVELGRSELGFERLAIWLVEDAESDMIRG
                     TFGTDAQGRTRDERDFVIRAPRSRFPLRRIDERVPKAYRKRMKRLVDWDKVITGGADT
                     LSSVITDGQKLIGYLVADTLISAREFSQGESEIFAFYASILGHLMQSRISGQVLQEQN
                     RQLELLNTITSALSGSRTPEQMGTQLFEIVRDLVACDSFILCQYDSQQQKLKAVVAYD
                     TIDGEWTELQRGNVFLNPTEDIRQMCFRREPLVVLRQNPELDSHGWKRNGDASRPSAS
                     LVFIPIISTDRVVGYYSVQSYTLNAYSQADVALLT"
BASE COUNT          989 a         1053 c         1242 g         1017 t
ORIGIN      
        1 aatgaaatca tgagcggcat caccggcagc gtggacgccg tggcgttcca ggacggtcat
       61 gtggactttg cccaactgcc cgagtttctt gagatcaaca gccacttggc acgcaagcac
      121 ggactgcact gctggacaaa ttcagagacc tttgatcgcg acatgccgat caagttcttg
      181 ccgatcaagt gggagaaact tttgctgaag ctccgggctg ccgaaaaggc gggtgttgag
      241 aaggcgatca cttttgagtt ctcacacttc atgagtccca actcttgtta ttgtcaggcg
      301 catgggctct accggagata ctgtgagcat ttcggcatcg tatgaggaga aacccgatgg
      361 atcgtgaaca gttcgcaaag tttgagactc tctatcagga cgctttcctg aatgatgtga
      421 ttcctttctg gatgaagcat tccctggatc gcgaacaggg agggtatttc acttgtcttg
      481 atcgggctgg cacagtctat gatcaggaca aattcatgtg gcttcagtgc cgacaggtct
      541 ggacgttctc catgctgtac aaccgactgg atcggcgttc ggaatggttg gatgtggcaa
      601 gcctgggctt tgagtttctg cgcaagtacg gcagagacgc cgaaggcaac tggtactttg
      661 cgctggaccg actgggcaga cccttggtgc aaccgtacag cattttctcg gactgcttcg
      721 ccgccatggg gttcagtcag tacgctcttg cctctggcaa cgaggaggca aaggagatcg
      781 cccttgccac atatcgaaat atcctcaaac gcaagaacaa tcccaaaggg aagtactcga
      841 aggtcgtgcc ggaaactcga ccgatgaagg attttgccct gccgatgatt ctctgcaacc
      901 tcggactgga aatgcagtgg cagctcgaca gcacagatct cgatcgaacc attgacatct
      961 gcattgatga agtgatgaat caatttcgag ataaggatcg ggggatcatc tttgagaaca
     1021 tcgctccgga cggttcccga ctggactgtt tcaatggcag actgatctgt ccaggtcatg
     1081 ggatggaagc catgtggttc attatggata tcggccgcaa acgcagcgat cggcagctga
     1141 ttgatcaggc cgtggatacc atgctgagcg ttcttgattt cggatgggat cgaaagcacg
     1201 gtggaatctt ctactttatg gactcagagg gcagaccgcc tcgacagctg gagtgggatc
     1261 agaagttgtg gtgggttcat cttgagtccc tggtggctct ggtcatgggc tatgctctga
     1321 cagagcgcac tgaatgctgg acgtggtatg agaagttgca tgactacact tggaaccggt
     1381 tcccagatcc ggacttcgga gaatggtttg gctatctgaa ccgacaaggg gacgttctcc
     1441 tgccactcaa gggcgggcag tggaaggggt gcttccatgt gccacgagcc ctgtatttgt
     1501 gttggcagga gttcaagaag ctgcgcgagg ggagacccca aacgaagtag tctcggtggc
     1561 tgacagggcg aacgcacagt cattgggcta caatttcatt ccttctgtcc agagcaggaa
     1621 tgtctactga ggatccacac atcttttgtt cctgacttgc atggggaagg agagcgtgtt
     1681 aattttccgt gtttatagaa cagcagagcc catcaatcgg gcttcgttcg accatcgaaa
     1741 aaggcaggtt gagagatgtc acttctgaac atgaccaata tccttcgggc tgcggctcga
     1801 agcggatacg ctgtcggtgc ttttaacgtt gtgaacttgg atttcctcga agggattctc
     1861 aacacggcca cacgctgcaa gtcgccggtc atcctgaaca tcgcggaagt tcattttcgc
     1921 tatgtggatc tcgatcagat cgtgcctgtc atccttcggg cagcggacaa ggtctctgtt
     1981 cctgtcgccc tgaacctcga tcatggtctg acttttgagg ccatcatgcg ggcgattcgc
     2041 tgcggattca cttcggtcat gttcgatggc tcgaaacttc ctctggaaga gaacatcgag
     2101 cgcacccgtc aagtcgtcaa gatgtgtcac gccctggaca tctctgtcga agccgagttg
     2161 ggacatgtgg caggcggaga aggctcgcgc gagggaaccg ctgtggaacg ggagttcttc
     2221 accaagcctg ccgaggcaat ccgtttcgtt cgggagacca acgtggacgc gctcgcgatt
     2281 gctttcgggt cggttcacgg acgttttaag ggcgatccaa agctggattt tgatctgctg
     2341 gatgagattc ggcgccatgt cggaattcct ctggtgctgc atggaggatc aggaatctcg
     2401 gacaatgatt tccgcaaagc gatctccctt ggcatcgcca agattaacat ctttaccgaa
     2461 atgactatcg ccgccacaga ccggatcaag cagaccttcc aaaaagatcc ggagattatc
     2521 tctttggcgg atctcatggt ggaagccaga aaagccatcg aggaaatcgt cgaggggaag
     2581 atgcgcgttt ttggaagcgc tggtgtctgt caaaacacga acgctttgtg cggcccctgc
     2641 caggcatgtg gattcagcgg gggagccacg cctcttgccg aagttcccaa agatgcgccg
     2701 gtgtcggaaa aggatttgat tgaaaccatc acccgtgttg tggtggaaac tcttcagcga
     2761 cagtgagaaa gagccatgat gacgattccc aaggatcttg aagaacggtg gcgacaagtg
     2821 gttgccaagt cattcaacga tgatcaagag gcggccttgc gtgaagcgtt gctgcggttc
     2881 attaccgaag aagagaaacg gattcccaag tcggggcagt ttgactcggt gctcagcaag
     2941 gtgcaacatc gtcagagcaa tctcgatgat atccttgcag acggcttgag cgatgccctg
     3001 gatcgcatga aaaagcgtaa agaacgcggt ctacctctct gaagaaacag ctgggtgccc
     3061 gttacgtgga tgagttttgc tgcgattgaa ttgaaggata ccaccctgcg aagagacagc
     3121 ggggtgtcca tgcagattgt tgacggcgga cgagttaagc gttttacccg ttgctgccgt
     3181 tctggactca cgatatgaaa gacaagccag gcaagaggaa aacacctgcc aagccttctg
     3241 atgcaccgaa ggctgattcc agaggcaggc gagcaaagga caaaaatacc gtgtgttccc
     3301 ctcggaagct cgcctcgtcg ccatcgaatt ccgttgccgt cgaaatctct cggctcgtgc
     3361 ttgtcggaag tgaattggcc gctattgaag acctgatgga tttgtgccgt cgcgctgtgg
     3421 agttgggtcg aagcgagttg ggttttgaac gcctggccat ctggctggta gaggatgcag
     3481 aatccgacat gatccgcgga acgtttggca ctgatgcaca gggcagaacg cgcgatgagc
     3541 gcgatttcgt catccgcgct ccccggtctc gattcccgct ccggagaatt gatgaacgcg
     3601 ttcccaaagc ctatcgtaag aggatgaaac gtcttgtgga ctgggataaa gtgatcacgg
     3661 ggggagcgga cacactttct tctgtaatca ccgacggaca aaaactcatc ggatatcttg
     3721 tggctgatac tttgatttcg gcgcgcgagt tctcccaggg cgagagtgag atttttgctt
     3781 tctatgcctc gatcctggga catctgatgc aaagccgcat ttctggtcag gtgctgcaag
     3841 aacagaatcg gcagctggag ttgcttaaca ctattacttc cgccctctca ggcagcagaa
     3901 cgccagagca gatggggacg cagctgttcg agatagtgcg agacctcgtc gcctgcgact
     3961 cgttcatcct gtgccagtat gattcccagc agcagaagct gaaggcggtt gttgcctacg
     4021 acacaattga tggcgagtgg acagaattgc agagaggcaa cgtgtttctt aatccaacgg
     4081 aagacattcg ccagatgtgt ttcaggcgag agcctctggt ggttctccgc cagaatccgg
     4141 agttggattc acatgggtgg aagcgaaacg gagacgcttc ccgtccttct gcttcgctgg
     4201 tttttatccc catcatctcg acagatcgtg ttgtgggcta ttactcggtt cagagttaca
     4261 cattgaacgc ctactcccag gcggatgttg ccttactgac c
//
LOCUS       DAOQUC010000148         4293 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_22950_length_4293_cov_3.118452, whole genome shotgun sequence.
ACCESSION   DAOQUC010000148 DAOQUC010000000
VERSION     DAOQUC010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4293
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22950_length_4293_cov_3.118452"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..278
                     /locus_tag="PKH07_03780"
     CDS             <1..278
                     /locus_tag="PKH07_03780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34097.1"
                     /translation="GWGSGTTSGGVWRLASSGPDRMQAWGGSTMEISTNAHQLGVDYD
                     PTNGTISGGDIVRVGSKAPTGNGMAPDDLSNPMRPGVLRVPFYREQW"
     gene            478..1509
                     /locus_tag="PKH07_03785"
     CDS             478..1509
                     /locus_tag="PKH07_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNT34098.1"
                     /translation="MMENKLGWGIIATGRIAGKFARALAHCDTGRLVAVTSRSKEKAE
                     QFGAEHGVSHCYGSYEEILADPEVDAVYISTPHPMHSEWAIKAAQAKKHILCEKPMGM
                     SRKEVEAMVDEAHVNDVFLMEAFMYRCHPQTAKLVELIRDGAIGAVRLIQATFSFQAN
                     VPPEHRLVNNALGGGGILDVGCYTVSMSRLIAGAALGKDFCEPSEVHGFGCFHPVTGV
                     DGYAVGELLFPEGIIAQIASGVLLAQENVVRIYGAKGSIVVPWPWGPSLEGGTTRILI
                     NRNGLPLEEYLVQTDQWLYAIEADTVAENIDRRQASAMSWADSLGNASTLDAWRKAVG
                     LVYQMEQHT"
     gene            1665..1805
                     /locus_tag="PKH07_03790"
                     /pseudo
     CDS             1665..1805
                     /locus_tag="PKH07_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011330557.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
     gene            1810..2034
                     /locus_tag="PKH07_03795"
     CDS             1810..2034
                     /locus_tag="PKH07_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017312579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34099.1"
                     /translation="MKKTDESCDLRPLYRRKDLGIGRRGKYLEAYRSGTNLVLLKPEV
                     AKAFPTEKAVNEALQSLIDIAQKSTGQLGH"
     gene            2115..3371
                     /locus_tag="PKH07_03800"
     CDS             2115..3371
                     /locus_tag="PKH07_03800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34100.1"
                     /translation="MVEQRVNSSVPETPKPEKKRLESLDAFRGLTIFVMVFVIVVAGY
                     RNLPQKMSWFGSVPISTWNHADVGWERFAEEKKAEGLSEEEIAQLPEARLKNIGCTIT
                     DLVAPFFVFIVGLCIPLSRARRGADWWRHVISRTVMLILAGVLYISLILGLSWWWGIL
                     QAIGIAYFMGVASMKLSRKGRWVAIFAILAFHMLMSHYVHWWLHFGETTEPFWKISQP
                     DGNWAKPLIVHCRPWVSISYGVMTMIGVLVGESLISRDRKTITKQALTLGAVFMVVGY
                     AVHKIGFITGQMGLCFNKPDVTASYAMFTSGIGAWTFLLIYYIMDVWGYRKWASPLVV
                     FGKNALLAYFMQIIMRIFFRALQIEDFFKGRNDTLVRWAEMLPSLETLLLSKSGYYGV
                     FWGLLWTFCLWLCVLYCNRKNIYWKL"
     gene            complement(3519..>4293)
                     /locus_tag="PKH07_03805"
     CDS             complement(3519..>4293)
                     /locus_tag="PKH07_03805"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HNT34101.1"
                     /translation="NPFGLPAGERGSWISEDVVKRKADVAYFAGCAVSYSQNRMAKAA
                     LRILDGAGIEYTTLGNDERCCGDPLLRMGLKDQAETMMEENRSNLQAKGVKTVFTSCA
                     GCAKSLKHSLGQDFEVYHVTEYLNKLASEGKIEFEKPFAKRVTYMDGCDLGRHAGVYE
                     SPRELLRHIPDIELVEMQQNRNLAVCCGGPFVAAYPELAKKFAANRIKDALAVGADTI
                     VVACPTCLLNLKEGAKSLDGAKVDIQEVVSLVQRSVKGK"
BASE COUNT          962 a         1070 c         1225 g         1036 t
ORIGIN      
        1 gtggatgggg tagcggtacg acttctgggg gagtgtggcg acttgccagt tctgggcctg
       61 acaggatgca ggcatggggc gggagcacga tggagatttc cacgaacgca caccaactgg
      121 gcgtagatta cgatccaaca aatggaacaa tcagtggtgg agatattgtc agagttggct
      181 ccaaagcccc tacaggcaat ggaatggcac ccgacgatct gtcaaatccc atgcgccctg
      241 gtgttctgag agtccccttc tacagagagc agtggtaaca ggattgggga agacttgctg
      301 ctcacaggta tcacagcaag attgtttcac cttgaatgta cttccttgtt catcaatcct
      361 ccttgcgaag cgagccgacg aggtctcctc atctgacatc cctttcatca gatgtgtttg
      421 acctatcaag gagaatctgg aaagataatc ttcgcaacgt cttgagcagg aggtttgatg
      481 atggagaaca aacttggctg gggaatcatc gccaccggac gcatcgccgg gaagtttgct
      541 cgagcacttg ctcactgtga caccggtcgg ctcgttgctg tcaccagccg ttccaaggag
      601 aaggcggagc aattcggcgc agaacacggt gtgtcccact gctatggatc gtacgaggaa
      661 atcctcgcgg acccggaagt ggatgccgtc tacatttcca cgccacaccc aatgcactcc
      721 gagtgggcga tcaaagccgc acaggcaaag aagcacatcc tttgcgagaa accgatgggg
      781 atgagtcgca aagaggtgga agcgatggtt gacgaagcgc atgtcaacga cgtgtttctg
      841 atggaagcgt ttatgtaccg atgccatcct cagacggcga aactggttga actgatccgc
      901 gatggagcca tcggcgccgt ccggctcatt caggcaactt tcagttttca ggcgaacgtg
      961 cctcccgaac accggctggt caacaatgcc ttgggcggcg gggggattct ggacgtggga
     1021 tgctacacgg tctccatgtc caggctcatc gcaggcgccg cactggggaa ggatttttgc
     1081 gaaccctcag aagttcatgg gttcggttgt tttcatcccg tgacgggtgt ggatggctat
     1141 gcagtcggag agttgctctt tcccgaaggg atcatcgccc agatcgcatc cggtgtgctg
     1201 ttggcgcagg agaacgtggt gcgaatctat ggcgccaagg gctccatcgt cgtgccttgg
     1261 ccttggggac cttctctgga aggtggcaca accaggattc tcattaaccg aaacgggctg
     1321 cctctggagg aatacctggt tcaaacagac cagtggctct atgcaattga ggcggacact
     1381 gttgcggaga acattgacag gcgtcaagcg tctgcaatga gctgggcgga ttcactgggc
     1441 aatgccagca ctctggatgc ctggcgcaaa gccgtcggtc tggtctatca gatggagcag
     1501 cacacgtagg aggacatcca gactgcccct cacaaaaggg gacacagagt gttcttgggt
     1561 ggacgtcatc tgatgcagct cgaccaagcc gatggaactt ggtttgctca gaaagtctgt
     1621 gatacagttt tagcatagta tagcaagcca cccggagtaa gagaatgcgg tttgcgtggg
     1681 actcgaacaa ggcaaattcg aacgtcagaa agcacgatgt gtcatttcag gaggctgcaa
     1741 ctgtttttgg tgacccgctg gcgatcactt ttgccgatcc cgatcattcc gagagcgaaa
     1801 ggagaatcta tgaagaagac tgatgagtct tgtgaccttc gtccgttgta tcgccgaaag
     1861 gatcttggca ttggacggcg aggcaagtat cttgaagcct acaggagcgg cacaaatctt
     1921 gtactcttga agcctgaagt tgccaaggcc tttccaactg agaaggcggt caacgaagca
     1981 cttcagtcgc tgattgatat tgcacagaaa tcaacgggtc agttgggtca ctgacagaac
     2041 ctggagttgc ataaaatggg cggattgcgc tcgacgagtg ttcctcgggt tgccatgcag
     2101 gaggtttaat gaagatggta gaacaaagag tgaatagttc cgttccggaa actcccaagc
     2161 cggagaagaa gcgacttgag tctttggatg cttttcgagg gctgaccatc ttcgtcatgg
     2221 tctttgtcat tgtggtggcg ggctacagga acttgccaca gaagatgagt tggtttggca
     2281 gtgttccgat ttccacgtgg aatcatgccg acgttggttg ggaacggttt gcggaggaga
     2341 agaaggccga ggggctgagc gaggaagaaa tagcgcagct gcctgaagcc cgactgaaaa
     2401 acataggctg tacgatcacg gatctggttg cgcccttttt cgtgttcatt gtgggtttgt
     2461 gcattccact gagtcgtgcc cgacgcggtg cagactggtg gcgtcatgtc atcagccgaa
     2521 cggtgatgct cattcttgct ggcgttcttt acatcagttt gattctggga ttgtcctggt
     2581 ggtggggcat tcttcaggcg attggcattg cctacttcat gggtgttgcg agcatgaaac
     2641 tgtctcgcaa aggtcggtgg gttgccatct tcgcaattct ggcgttccac atgctcatgt
     2701 cgcactacgt tcattggtgg ctgcactttg gcgagacaac cgagcctttc tggaaaatca
     2761 gccagcctga cggcaactgg gcaaagcctc tcatcgttca ttgtcggccg tgggtctcga
     2821 tttcctatgg cgttatgacg atgatcggtg tgttggttgg ggaatctctt atcagccgtg
     2881 accgcaagac gatcacaaag caggcgctca ctcttggtgc ggttttcatg gttgtcggat
     2941 atgcagttca caagattggt tttatcaccg gtcaaatggg gctctgcttt aacaagccgg
     3001 acgtgaccgc atcctatgcg atgtttactt caggtatagg agcctggacg tttctcttga
     3061 tctattacat tatggacgtc tggggatacc gcaagtgggc atctcccttg gtcgtttttg
     3121 gaaagaatgc gctgctggcc tatttcatgc aaatcatcat gcgcatcttt ttccgtgcgc
     3181 ttcagatcga ggacttcttc aaggggcgca atgacacctt ggttcgatgg gcggaaatgc
     3241 tcccgtcgct tgagacactt ctcctgagca agtctggtta ctacggcgtc ttctggggac
     3301 tgctttggac gttctgtttg tggctttgcg ttctgtactg caaccgcaag aacatctact
     3361 ggaaactcta gaagaagatg gcgtggatgc cgcagtgcag catccctcgt gctgttgttc
     3421 tggagcagac aaacgaaagc acgacaacaa caaacgcggt cagtcacgat gcaatgcaat
     3481 cagcagcggc ggctctttcc ctgatgcggg gcaagcgttc acttgccctt gacagagcgc
     3541 tgaaccagag acacgacttc ctgaatgtct accttggcac catcgaggct ttttgcccct
     3601 tctttgaggt tcagaaggca tgtcggacag gcaacaacga tggtgtctgc accgacggcc
     3661 aaggcgtcct tgattctgtt tgccgcaaac ttcttggcaa gttccggata ggcggcgaca
     3721 aagggaccac cgcaacacac agccagattc cggttttgtt gcatctcaac caattcaata
     3781 tcaggaatgt gtcgaagtaa ctctcgtgga gactcataga ctcccgcatg acgaccgagg
     3841 tcacagccgt ccatgtaggt cactctcttt gcgaagggct tttcaaactc aatcttgcct
     3901 tctgatgcca gtttgttgag atactctgtc acatggtaga cctcaaagtc ttgacccaat
     3961 gagtgtttga ggctcttggc gcatccggcg caggacgtga agacagtctt cacccctttc
     4021 gcttggaggt tgctgcggtt ctcctccatc atggtttccg cctgatcctt cagtcccatc
     4081 cgaagcagtg gatcaccaca acagcgctca tcattgccaa gagttgtgta ctcaatgccc
     4141 gcgccgtcca gaatccgaag cgccgccttg gccattcggt tctgggagta cgacacagca
     4201 caccctgcga agtaagccac atccgccttg cgtttgacaa cgtcttcaga gatccaggaa
     4261 ccgcgctcgc cagcaggcag tccgaaggga ttt
//
LOCUS       DAOQUC010000149         4293 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_22951_length_4293_cov_2.997640, whole genome shotgun sequence.
ACCESSION   DAOQUC010000149 DAOQUC010000000
VERSION     DAOQUC010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4293
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22951_length_4293_cov_2.997640"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..752)
                     /locus_tag="PKH07_03810"
     CDS             complement(<1..752)
                     /locus_tag="PKH07_03810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34102.1"
                     /translation="MRYLSLMLALFATHLPCVTPSSFAEEVVSRVMSNERATLYMGGT
                     PAGEQTLRVEKTSLPSDGSTPTKEGYLFVSTAHLQILAMGVVIPLDVNTECVTNDDFS
                     VHSFDFNMESAGTQISMSAHVEDETLSLQLTSAGSVSRQEISLAEGPVFTPEAIYMAM
                     SREGLEVGTQKSFRVYDPMSTSLDTITGVVLKKDSIYFGGKPVETYLLTLDFKGFQQK
                     AWIDTDGVCYQEESEVAGQNFVALRTLDDGTTV"
     gene            complement(843..2906)
                     /locus_tag="PKH07_03815"
     CDS             complement(843..2906)
                     /locus_tag="PKH07_03815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34103.1"
                     /translation="MNLTFDRRIGSRGSLFVLIFVLVAQSSLCQAEILGSCYRKDEPF
                     PEYMPLWSEGYADRDQVDQYTYEGMPLGASLHFYYRNVTSSTVQVSDVLIGGISLLEA
                     IQYSEDKARKHAQPASIEFSKLSEDQKKQIKASGEPIWWKVEPRSIKAGATAEITVRL
                     RKRPLQESVALTLVTDKGKEEATIAVADTIGPRVHGVSFSDDRRRLFLYLRHPNGGVT
                     PPNALYVDGEDVTGRTTLGQDNSYAIVPVEILLDNPFERASFHCARAEYQDGSSAWAG
                     FRVWDDELAFGIWGARPGAQEAVDIAKAYLQDIAQHNINVQMEMVGSEAVSAFLKSDE
                     GRQEMQRLGIRRMIADYGKGNTQNPYAYFLVDEPDAGDYTVESLDIRSRVGALAQSLV
                     RKGEQLRESDPATPNLVNVNSTFKPENWYVYGQVPDIFATDPYYQARLLEVFWRRPER
                     YPLFGKATIIEAVSKISHSACAPKTLHLVLNAVSHRNKQGRVFRFGTPEEKRIETYYA
                     LSAGAKGFSYWWYTPGGQSHGCGAQEPEAVALWREIGLLGAEIRTLGSLIQGGCPVDL
                     PLTSPNNLSVNGIMATNRALVLICINENYFNDRLGTIYSPVRDAVVSLTLPIWLNARE
                     VFEVSFEGTKDVSFADQQEVLKLQLGTVNVTKLVVVTSDLKLRTKLQEYYVTHLKDNV
                     ASLLK"
     gene            complement(3008..4147)
                     /locus_tag="PKH07_03820"
     CDS             complement(3008..4147)
                     /locus_tag="PKH07_03820"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HNT34104.1"
                     /translation="MRFTIEDSPVPKYVQLRDQILLAIESGRLSVGDQLPTEDALVRE
                     LGISRNTVRQAMADLLQRGQVTRERGRGTFVALQRTRHLDSESPTSRAIGVLVRDIAS
                     QDDVYPEIIRGIQDVCSQQDYHVILANTDARLDRMAEGMKSFLKAGVSGTIISPAMDQ
                     MAMTTSQLRQSFKESLELYNRFLDSGIPIVLINRRVPNLPVPCVLSDNELGGYLATKH
                     LLDQGHRRIAALFPPVYSTVKARETGYRKALAEAGIAPAEGWVQFACLDDPDPVRTMV
                     DAVTKLSSPPTAIFAFTDDYAAKIFERLMELGLRVPEDVALVGYNDCRLALSLPVPLT
                     SVAYPKYEIGQKAAQMLLRHPRPTGEASSIVLPPTLIVRNSSVRG"
BASE COUNT         1016 a         1108 c         1121 g         1048 t
ORIGIN      
        1 acggttgttc cgtcatccag tgttctcagc gcaacgaaat tctgaccggc aacctcggac
       61 tcttcctggt agcagactcc gtcggtatct atccaggcct tctgctggaa tcccttgaaa
      121 tcaagtgtga gaagatatgt ttcaacaggc tttcccccga aatagataga gtctttcttg
      181 agcacgactc ccgtgattgt gtccagtgaa gtggacatgg gatcgtagac tcgaaacgat
      241 ttttgggtac cgacctcgag tccctctctc gacatggcca tgtagatagc ctcgggcgtg
      301 aagaccggac cttctgccag actgatctcc tgacgagaga cggaaccagc ggaggtgagc
      361 tgcaggctga gtgtttcatc ttctacatgg gctgacattg aaatctgtgt gccggcggac
      421 tccatgttga aatcgaatga gtgtacggaa aagtcatcat tcgtaacgca ttccgtattg
      481 acatcaaggg gtataacgac acccatggca agaatctgaa gatgcgccgt gctgacaaag
      541 agataaccct ctttggtggg tgttgagccg tcagagggaa gggaagtctt ctcgactcga
      601 agcgtctgtt cgcctgccgg tgtccccccc atataaagtg tggcacgctc gttagacatg
      661 actcttgaga cgacctcctc ggcaaaggaa gaaggggtga cacaaggaag atgcgttgcg
      721 aacaaagcaa gcatcaggga caaatatctc acaggctact ccttcctgtt gcagcgtcag
      781 aataggcacc gtacggcaca catggaaagg atagcccatg caggctggtt cggcaacagg
      841 atctacttca acagactcgc aacgttatcc ttgagatgcg tgacatagta ttcctgcagc
      901 tttgttcgaa gtttcagatc ggaggtaaca accaccaact ttgttacatt cacagtcccc
      961 aattgcagtt tcagcacttc ttgttgatcg gcgaaggaaa catccttggt gccctcaaag
     1021 gagacctcga aaacttcgcg ggcgttgagc cagatgggaa gagtcaggga gactactgca
     1081 tctctgacgg gtgagtagat cgttcccagt cggtcgttga aatagttctc gttgatacag
     1141 atgagaacaa gagctcgatt tgtagccatg attccattca cagaaagatt gttgggcgat
     1201 gtcaggggta agtcaacagg gcatccacct tggatcaaag agcccagcgt tcggatttct
     1261 gctccgagaa ggcctatctc acgccacaac gcgacagcct caggctcttg agcaccacat
     1321 ccgtgacttt gtccaccggg tgtgtaccac caataggaga atcccttcgc cccagccgac
     1381 agggcataat aggtttcgat tcttttctct tcaggggttc caaaccgaaa gactctgcct
     1441 tgcttgtttc tatggctgac agcgttcagc acaagatgca gggtttttgg cgcgcaagcc
     1501 gagtgggaaa tcttgctgac cgcctcaatg atcgtcgcct ttccaaaaag gggatagcgc
     1561 tctggccgtc tccagaagac ttccagaagt ctggcctggt agtaaggatc ggtggcaaat
     1621 atatcaggca cctgaccata gacgtaccag ttctcaggct tgaaagtcga gttgacattt
     1681 accagattgg gtgttgctgg atccgactcc ctgagttgct ctccctttct gaccaacgac
     1741 tgagccaatg ctccaactct tgagcgaatg tcaagactct ctaccgtata gtcaccagca
     1801 tctggttcgt ccaccaggaa gtaggcgtat gggttctgag tattgccctt tccgtagtcg
     1861 gcgatcatcc ttcgaatacc cagacgctgc atctcctgtc tgccttcgtc acttttgaga
     1921 aaggccgaaa ccgcttcgga accaaccatt tccatttgga cattgatgtt gtgttgcgcg
     1981 atgtcctgaa ggtatgcctt agcgatatca acggcctcct gcgctcccgg tcgtgctccc
     2041 cagattccaa aggcaagttc gtcatcccaa acacggaaac cagcccatgc agatgaccca
     2101 tcctggtatt ccgcccgtgc gcagtgaaaa gaggctctct caaatgggtt gtcgagcagt
     2161 atctcaacgg gaacgattgc ataggagttg tcctgtccca gcgttgttct gccggtgaca
     2221 tcttctccgt caacataaag cgcattgggt ggtgtgactc caccgttcgg atgacggaga
     2281 tacaggaaaa gtcgtcttcg gtcatccgag aaggagacgc cgtgcactcg aggaccgata
     2341 gtgtccgcga ctgcgatggt tgcctcttcc ttccctttgt cagtaacgag cgtgagtgcc
     2401 accgattcct ggagagggcg ctttctgaga cggacagtga tctcagccgt tgcaccggct
     2461 ttgatgctcc ttggctcgac cttccaccag atcggttctc cagaggcttt gatctgcttc
     2521 ttttgatcct ctgaaagctt tgaaaactcg atgctggcag gttgcgcgtg ctttcgagcc
     2581 ttgtcttcag aatactgaat tgcttcgagg agactgattc ccccgatgag aacgtcactt
     2641 acctgaaccg ttgaggatgt aacattgcga taatagaagt gcaacgaagc tcccaatggc
     2701 attccctcat aggtatactg gtcaacttgg tcacgatcgg catagccctc agaccagagt
     2761 ggcatatact caggaaaggg ttcatccttt cgataacagg agccgagaat ctccgcttgg
     2821 cacagagaac tctgcgcaac cagaacaaag atgagcacaa aaagcgaacc tctcgaaccg
     2881 attcttctgt caaatgtcag attcatctcg tcctccattg aggtgcagtc acaccaatct
     2941 gtttcaaacc ggagcaaatg tcaatcgtgc gctgagtatc gggttcattc tgttgtctgc
     3001 tggatggtta gccccgtacg ctggagttgc gaacaatgag agtcggtggc agaacaatcg
     3061 aagacgcttc acccgttggt ctcggatgcc tgagaagcat ttgcgcggcc ttttgtccaa
     3121 tttcatattt gggatatgcg acagaggtca acgggactgg aagagagagt gcgagtcggc
     3181 agtcgttgta tccaacgagc gctacatctt cagggacacg caggccgagt tccataagtc
     3241 tttcgaatat cttggcggca tagtcatccg tgaacgcaaa gatagcggtt ggtggagaag
     3301 agagtttcgt cactgcatca accatcgttc tgactgggtc tggatcgtca aggcatgcga
     3361 actgaaccca gccttccgcg ggcgcgatac ctgcttcagc gagtgccttt cgatagcctg
     3421 tctcacgggc tttgaccgtt gagtagacag gaggaaacaa tgctgcgatt cgacggtgtc
     3481 cctgatccaa gagatgtttt gtcgccaggt agccacccaa ctcgttatcc gaaaggacgc
     3541 agggaacagg aagattcggc actcgcctgt tgatgaggac gatgggtatg ccggagtcca
     3601 gaaaccggtt atacagttcg agagattctt tgaacgactg acgcagctga ctggttgtca
     3661 ttgccatctg atccatcgcc gggctgatta tggtgcctga gacacccgcc ttgaggaaac
     3721 tcttcatccc ttcagccatc ctgtcgagcc tggcgtccgt gttggcaagg atgacgtgat
     3781 agtcctgttg gctacagacg tcctgaatcc cacggatgat ttcgggatag acgtcatcct
     3841 ggctggcaat atctctgaca aggacaccga ttgctcttga agtcggtgat tcgctgtcga
     3901 ggtgccgtgt cctctgcaat gccacgaaag tgccgcgacc tcgttctcgt gtgacttgtc
     3961 cacgttgaag gagatcggcc atagcctggc gaacggtatt gcgactgatg ccaagttctc
     4021 gaaccagagc atcttctgta ggaagctgat cacccactga gagtcttcca gactcgattg
     4081 ccagaagaat ctgatctcgg agctgaacgt atttcggtac tggggaatct tcgatggtga
     4141 aacgcatttg tatgaactac ttcctacacg tcttcaatac agtattccta ccgaactcaa
     4201 cccatggttg tcaacataaa tccccgaaaa ctccaatggg catagggtga tccgcggtgc
     4261 agcatctgga gactgtgtgc attccggaga cca
//
LOCUS       DAOQUC010000150         4270 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_23117_length_4270_cov_3.249348, whole genome shotgun sequence.
ACCESSION   DAOQUC010000150 DAOQUC010000000
VERSION     DAOQUC010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4270
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23117_length_4270_cov_3.249348"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            64..972
                     /locus_tag="PKH07_03825"
     CDS             64..972
                     /locus_tag="PKH07_03825"
                     /inference="COORDINATES: protein motif:HMM:NF013335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HNT34105.1"
                     /translation="MSMKVILDTDMGDDIDDAYALALILASEEFDLLGVTTVFGNTPA
                     RTQQALTLLQIAGRSEVPVATGAPGVLSPRLHYKFTPKENYLKGNLPCQHSSSLPWEK
                     LPAPSAMDAPRFIVEKVMNGDGDITVITIGALTNLALATVLEPKICAKIPRVVCMGGF
                     FDQPVAEYNINCDPIAASLVFESGLPMTVVGLDVTLKCQFTDEDLSRLYQSTRPLACN
                     LADATRAWTGKYPVLHDPLAVETLIRPDLVITRNGNVRVSCTEEATYGFTVFQSSADN
                     SGTHDVGFGVKHREAIDLWLERVLNY"
     gene            985..4155
                     /locus_tag="PKH07_03830"
     CDS             985..4155
                     /locus_tag="PKH07_03830"
                     /inference="COORDINATES: protein motif:HMM:NF016563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH116 family glycosyl hydrolase"
                     /protein_id="HNT34106.1"
                     /translation="MKKQPSSKDRKRCRALQEHGIAPSGSPSCHEPSCCCNMNRRRFL
                     KTAGTAAAFFPTLGILNAATPRADTGATEVHPSHLVPVEKNLPDDYLNTLLERGSSEW
                     HRDKSLDMIGMPIGGIAAGQLYLLGDGTLGCWNIFNHVPNTGSGDRCYNNQKQVKPVD
                     QGFALVVEDGGRTTVHTLSREGFSDIAFLGEYPIGKVTYADPDCPIRITLEAFSPFIP
                     LNSEDSGLPATIFHMDLENVSKKPVKAGILGWLENAVCIGSKVGYGRTRTYVSKSSST
                     VSHSVEPPPKSVRQDLRPVIVFEDFEGSDYGTWSIEGEAFGSSPAKGTLPNQSNVSGF
                     IGAGLVNTYLGGDKTQGTLTSPVFRIERKYINFLVGGGNHEGQTCVNLLVDGNILRSA
                     VGSQNEALQWHSWSVEDLEGKQAQIEIVDRAMGAWGHINVDQIEFADQSAPFERKPLR
                     EVPDYGSLALTFLGECSSTEDLSRICALQFPKTTEQRICSNATYPIMKRNIGALLSLM
                     SELAPGQSIRYSSVLSWHFANAPEGRFYATRFQSARHVSRYVAKNCKRLTQQTKLWHD
                     VYYDSTLPYWLLDRLIYTVSNLATETCQWWRNNRFWAFEGVGCCHGTCTHVWNYAHAL
                     ARLFPSLEKSVREMQDLGIALRDDGMVAFRGEAKGNYAADGQAGTILKCYREHLMSPD
                     NTFLKRNWEKIRRVMDFSILQDENANGLIENSQHNTYDLNFEGANTFVGSLYLAALRA
                     TEMMARDMKDLAYAKKVRQIFESGSRLTVERLWNGEYFVQDVDLEKVNGQYGDGCLSD
                     QVFGQGWAHHVGLGYIYPQTCVRGALQSVWKYNWAPDVGPQTSKHVPWRYFANEGEAG
                     LMLCTWPKSQHPGERSVYYRDEVWTGIEYQVAGHMVWEGMLTEALSIVRAIHDRYDPL
                     KRNPYNEVECGDHYARALASWGVFTALAGYEYHGPKGFIAFGPKVTPENFRAAFTAAE
                     GWGTFSQTRSGSMQRNTVEVRLGRLDIKELGLKVPQGWAVDKAQVMINDTPIRTSLTV
                     YEDRVLVSFRRKIQIKDGDVLSILIE"
BASE COUNT         1024 a         1139 c         1156 g          951 t
ORIGIN      
        1 cgcttgatcg tacaggatac cgtctgaagc caaatctact gagcgcagag gagaacaact
       61 ggaatgagca tgaaggtgat actcgacaca gacatgggtg acgacattga tgatgcctat
      121 gcattagccc tgattctggc atcagaggaa tttgacctgc tgggcgtcac taccgtattt
      181 gggaacaccc cagcccgaac gcaacaggca ctgacactgc tgcagatcgc cggccgctca
      241 gaagtacctg tcgcgactgg ggctcccgga gttctctctc ccagactcca ctacaagttc
      301 actcccaagg agaactatct caaaggaaat cttccctgcc aacactcaag cagcttgcca
      361 tgggagaagt tgcctgcccc gtcggcaatg gatgcgcctc ggtttatcgt cgagaaggtg
      421 atgaacggcg acggtgacat cacagtcatt acgatagggg cgctcaccaa tcttgccttg
      481 gcgacagtgt tggaacccaa aatctgcgcc aaaattccaa gggtcgtctg catgggcggt
      541 ttctttgatc agcccgttgc cgaatataac atcaactgtg acccgatcgc cgcctccctg
      601 gtctttgaga gtggtctccc catgacggtc gtgggtctgg atgtcaccct gaaatgccag
      661 ttcacggatg aagacctctc acgactgtac caaagcacaa gacccctggc ctgcaatctt
      721 gctgacgcaa cacgcgcctg gacgggcaag tatcctgttc ttcacgatcc cctcgccgtt
      781 gagactttga tccgtcccga cctggtcata acgagaaacg ggaacgttcg ggtttcctgc
      841 acggaagagg caacctacgg cttcaccgtc tttcagtcct ctgcggacaa ctccggtacc
      901 catgatgtgg gattcggtgt gaaacatcgg gaagcgattg atctctggct tgaaagagtc
      961 ttgaactact gacggaggca aaccatgaag aagcaaccat ccagcaaaga ccgcaagcga
     1021 tgtcgcgcac tgcaggaaca tggcatagcg ccgtcgggtt caccttcgtg ccatgagcca
     1081 agttgttgct gcaatatgaa ccgcaggcgc ttccttaaga cggcaggcac cgccgccgca
     1141 ttctttccca ctctcggcat cctaaacgca gcgactccca gagccgacac aggcgcgacc
     1201 gaagttcatc catctcacct cgtccctgtt gagaagaatc ttccggacga ttacctgaac
     1261 acccttttgg agaggggttc ctcggagtgg cacagggaca agagtctgga catgattggt
     1321 atgccgatcg gaggcatcgc tgcaggacaa ctctacctcc tcggtgacgg aactctaggc
     1381 tgttggaaca tcttcaacca tgtgcccaat accggttcgg gcgatcgctg ctacaacaat
     1441 cagaaacagg taaagccagt tgaccaggga tttgctctcg ttgttgaaga tggagggaga
     1501 acaaccgttc acacattgag ccgagaagga ttttccgaca ttgcattcct gggtgagtat
     1561 cccattggga aggttaccta cgccgatccc gattgtccca ttcggattac actggaggct
     1621 ttttcgccat tcatcccgct caactcagag gactctggac tccctgcaac aatctttcac
     1681 atggatttgg agaatgtgtc gaagaaacca gtcaaagcag gtattcttgg atggctcgag
     1741 aatgccgttt gcattggctc caaagtggga tatggaagaa cccgaaccta cgtgtcgaag
     1801 tcatccagca cggtttctca ttcagttgag cctcctccca agagcgtcag acaggatttg
     1861 agacctgtga tcgttttcga ggacttcgaa ggttccgact atggaacatg gagcattgag
     1921 ggagaggcct ttggctcttc tcccgcaaag ggaacgcttc ccaatcagag caacgtctcc
     1981 ggattcatcg gagcaggtct tgtcaatacg taccttgggg gagacaagac gcagggcaca
     2041 ctgacctcac cagtcttccg catcgagcga aagtacatca actttcttgt gggtggtggg
     2101 aatcacgagg gacaaacctg tgtaaacctt ctcgtagatg gcaacattct acgaagtgcg
     2161 gtcggaagcc agaatgaggc gctccaatgg cattcctggt cggttgagga cttggagggc
     2221 aagcaagcac agatcgagat cgtcgatcgc gcgatgggtg cctggggtca tatcaatgtg
     2281 gatcagatcg agttcgccga ccaatctgca ccttttgagc gcaagccatt gagagaggtc
     2341 cccgactatg gttcccttgc gttgacattc ctcggagagt gctcttcgac cgaggatttg
     2401 tcgcgtatct gtgctcttca gttccccaag accacagaac agcgcatctg ctcaaacgca
     2461 acatacccga ttatgaagag gaacataggt gcactgctga gcctcatgtc tgaactcgcc
     2521 cccggccaaa gcatccgcta ttcatctgtg ctcagctggc atttcgcaaa cgccccggaa
     2581 ggcaggttct acgcaacccg attccaaagc gccagacacg tctcgcgata tgtcgccaag
     2641 aactgcaaga gattgaccca gcagacgaaa ctttggcatg acgtttacta tgacagtacc
     2701 cttccctatt ggctcttgga tcgtctcata tacacggttt ccaatctcgc aacagagacc
     2761 tgccaatggt ggaggaataa tcgtttctgg gcatttgaag gagtgggctg ctgtcacgga
     2821 acatgcaccc atgtctggaa ctacgctcat gcgttagcaa ggttgttccc ctcactcgaa
     2881 aagagcgttc gcgagatgca agatctcggc attgcactgc gagatgatgg catggttgct
     2941 ttccgcggtg aagcaaaggg aaactacgcc gccgatggtc aggcaggtac gattctgaaa
     3001 tgctaccgcg aacatctgat gtcgcccgac aatacttttc tcaagcgcaa ctgggagaag
     3061 atccgcaggg tcatggactt ctcaatcctt caggatgaga atgccaacgg cctgatcgaa
     3121 aacagtcagc acaataccta cgacctcaat ttcgaaggag ccaatacgtt tgtcggttcc
     3181 ctgtaccttg cggcgcttcg agcgacagag atgatggcca gggatatgaa ggacttggcc
     3241 tacgcgaaaa aggtcagaca gatattcgag agtggttctc ggctgactgt cgagcgcctt
     3301 tggaatggcg aatacttcgt tcaggatgtt gacctcgaaa aggtcaatgg gcagtatgga
     3361 gacggttgcc tgtccgatca ggtctttggt caaggttggg cacaccatgt cggtctgggg
     3421 tacatctacc cgcagacctg tgtcagaggg gcgctccagt cggtctggaa gtacaactgg
     3481 gctcccgacg tcggaccgca gaccagcaaa cacgttccct ggcggtactt cgccaacgaa
     3541 ggagaggccg gtcttatgct ctgcacctgg ccaaagagcc agcatccagg tgaacgttcg
     3601 gtctactacc gggacgaggt ttggacaggc atcgagtatc aggtcgccgg acacatggtc
     3661 tgggaaggaa tgctgactga agcactctcc attgttcggg cgattcatga ccgctatgat
     3721 cccctcaagc ggaatccata caacgaagtc gagtgtggtg atcactatgc ccgagccttg
     3781 gccagttggg gagttttcac tgcgctcgcg ggttatgaat atcacggacc caagggattt
     3841 attgcattcg gccccaaagt cactcctgag aactttcgcg cagcctttac cgcggccgag
     3901 gggtggggaa ccttttcgca gactcgttcg ggcagtatgc aacgaaatac cgttgaagtg
     3961 cgcttgggtc gccttgacat caaggaactg ggactgaagg ttcctcaggg atgggctgta
     4021 gacaaagctc aagtcatgat taacgatacg ccgattcgga catctttgac tgtctacgaa
     4081 gaccgtgtgc ttgtgtcttt tcggcggaag atccagatca aggatgggga cgtgctctcc
     4141 atcctgattg agtaaagtgt cgtgacttgc tgaaccgtcg ggctggaagc ggcctcgtcg
     4201 tcaagacaca aagggagtcg aaaaatgcat ggaaccatca tgttgggcgt tgacgtggaa
     4261 tctccccacg
//
LOCUS       DAOQUC010000151         4269 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_23122_length_4269_cov_3.533460, whole genome shotgun sequence.
ACCESSION   DAOQUC010000151 DAOQUC010000000
VERSION     DAOQUC010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4269)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4269)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4269
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23122_length_4269_cov_3.533460"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..963
                     /locus_tag="PKH07_03835"
     CDS             <1..963
                     /locus_tag="PKH07_03835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34107.1"
                     /translation="EPRAFDSLEMRWLDPLERAASAEYDLSKRYFGGESFPFVDTHIG
                     PGSLATMLGSKPGFHWNTVWYNPCIADLENHPPLAFDPDSEWFKKHVAILEESVCRSR
                     GRYLVSMPDLIENIDTLASLRGSEQLMMDLIEQPEIVHRRLAEINQVYFQVFDALYER
                     IKTPWGGNVFSAFCIWGPGKTAKVQCDAAAMISPDMFGEFVVPYLTEQCQWLDYALFH
                     LDGVQCICHLDQLLAVAPLKAIQWTPQAGVPPVGDSRWFDLYKRILDGGKSVQILGIQ
                     PERVEPVLKAIGTKGVFMSVFLHTEFEARSICELVERYRSVERA"
     gene            960..2063
                     /locus_tag="PKH07_03840"
     CDS             960..2063
                     /locus_tag="PKH07_03840"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNT34108.1"
                     /translation="MTMEYDSRYKAFMGLAPRQIPHWEHWSCPDAESYLTGIDYYEHP
                     RQCRLKLRELYPQLSLPVPESDEPKARPRLDGTHHSSLLDAEGRRRVRWGESETNHWQ
                     WGIQFHSAEEVFAFSPLANADFTKIPVVESRDYSDEEKLFEMYRKLFPAEHGERAPEG
                     STAQVGFYNTMFMWPLLTFGWELFLETCLDPRFDRIMEEFAEINRRVFRVFSRLPVNF
                     VVCHDDIVTTRGPVCSPAWMHKHIFPRYEEFWGILRAAGKQVIFMVDGCMDAYADDVF
                     SCGARGIITEPYTDFKTIARKHKDCFLAGEGDNRILTRNNPEEIRSMVKSMVETAHLT
                     GGYMMCIGNHIPWDIPPEAIKRYLDLSVEMAHR"
     gene            complement(2075..3238)
                     /locus_tag="PKH07_03845"
     CDS             complement(2075..3238)
                     /locus_tag="PKH07_03845"
                     /inference="COORDINATES: protein motif:HMM:NF025957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HNT34109.1"
                     /translation="MNKTTLFKTAFRSSRSSSSTRAKILFILFFLLSILPMALIIAKA
                     FGQEETTMSWHSVSEPVFRINGLPFWAENEGEFWRFPKRAQSLVPEGAWIQSRYPSGG
                     RIRFKTDSTSLAIRVEYMTLPNMRNMHAFGQSGVDAYVDGQYVASVAPRKDIQVEEHF
                     FKGQEKKEREITLYLPLYMGASVKGIGIDPDASLDAASDFSVERPVVYYGTSITQGGC
                     ASRPGLSYQAILGRMLNVDFVNFGFSGAGKGEPEVAQIISEVSASCFVLDYTQNTKDA
                     ETLEKVYIPFIEVIRSRHPETPIICTTAIYMAPEAVFSNVHPDLPRTREVIRKAVQAR
                     KDAGARNIHLVEGFTLLGPDQADGFVDGVHPNDLGFYWMAQGLEPHLRKALGL"
     gene            complement(3272..3973)
                     /locus_tag="PKH07_03850"
     CDS             complement(3272..3973)
                     /locus_tag="PKH07_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011366811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNT34110.1"
                     /translation="MGEETIHALNGVSVSVKRGEYVSIMGPSGSGKSTLFNMIGGLDK
                     PEEGGRVFIDEVNIAALDTFELAWLRCHKIGYIFQTFNLIRVASALENVMFPMLFAGM
                     TQADAQDKAATLLERVGLGDRMLHKPAELSGGQQQRVAVARALANDPSIILADEPTGN
                     LDLTTGQEIIEILFRLTKDCDVTVITATHDHKMLTCSDRVIYIADGKVNSIKDRDELN
                     IQVGGIHATDSKKKN"
BASE COUNT          996 a         1108 c         1157 g         1008 t
ORIGIN      
        1 gaaccacgcg cttttgactc tctggaaatg cgatggctgg atcctttgga acgcgctgca
       61 agcgctgagt acgacctttc caaaagatac tttggaggtg agtcctttcc gtttgtggat
      121 acacacattg ggccgggaag tcttgccacg atgttgggat ctaagccggg ttttcattgg
      181 aacaccgtct ggtacaaccc ctgcattgcg gatctggaga atcacccgcc gctggcattc
      241 gatccggaca gcgaatggtt caagaagcat gttgcaatac ttgaggaatc tgtctgtcgc
      301 agtcgaggtc ggtatcttgt ctccatgccc gatctgatcg agaacataga cactctggct
      361 tcattgcgag gttccgaaca actgatgatg gacttaatcg agcaacctga aatcgtgcat
      421 agacgtcttg ccgagatcaa ccaggtttat tttcaggtct ttgacgcgct ctacgagcgg
      481 atcaagaccc cttggggtgg caatgtgttc tcggcgttct gcatctgggg gcctggcaag
      541 acagcaaaag tgcaatgtga tgccgcggcc atgatctcac ccgacatgtt tggtgaattc
      601 gtcgtgcctt acctgactga gcagtgtcag tggctggact acgccttgtt tcatctggat
      661 ggcgtgcagt gcatctgcca tctggatcaa cttttggcgg ttgcgccctt gaaggccatt
      721 caatggacgc ctcaggcggg tgtaccgccc gtgggtgatt cgagatggtt tgatctctac
      781 aagcgcattc ttgatggagg aaagtcggta cagattctcg gaattcagcc ggaacgcgtg
      841 gaaccggtgc tgaaggctat tggcaccaag ggagtgttta tgtccgtgtt tctgcacacg
      901 gagttcgagg ctcgttcgat ttgcgagctg gttgagagat acaggagcgt ggagagagca
      961 tgacgatgga atacgatagc cgatataaag cattcatggg tctggcaccc agacagattc
     1021 cgcactggga gcattggtcc tgtcccgatg cggagtctta cctcacagga attgactact
     1081 atgagcatcc tcggcagtgt cggctgaagt tgcgtgagct gtacccacaa ttgtctcttc
     1141 ccgttccgga aagtgatgag ccgaaagcca gaccccggct ggacggaacg catcatagtt
     1201 cgctcctgga cgccgaaggt cgtcgtcgag tgcgatgggg agaatctgag accaaccatt
     1261 ggcaatgggg gattcagttt cactcggcag aagaggtctt tgccttttcg ccactcgcga
     1321 acgccgattt cacaaagatt cccgtggtcg aatcacggga ctattctgat gaagaaaaac
     1381 tctttgagat gtacagaaaa ctctttccgg cggagcatgg agaaagggca cccgagggat
     1441 cgactgccca agttggcttc tacaacacaa tgtttatgtg gccgctcttg acattcggat
     1501 gggaactctt ccttgaaacc tgcctggatc ctcggtttga ccgcatcatg gaggagtttg
     1561 ctgagatcaa ccgccgagtg ttccgtgtct tctcacggtt gcccgtgaat tttgttgtgt
     1621 gtcacgacga catcgtcact acgcgaggcc ccgtttgttc ccccgcctgg atgcacaagc
     1681 atattttccc tcgctacgaa gagttctggg gaatcctgcg cgcagccggg aaacaagtga
     1741 tcttcatggt ggatggttgc atggatgcct acgcggacga tgttttttcc tgcggagcac
     1801 gaggaatcat cacggagcct tacaccgatt tcaagaccat tgcgcgaaaa cacaaggact
     1861 gttttcttgc cggcgaggga gacaaccgaa ttctcacccg caacaatcct gaagaaatcc
     1921 gttcgatggt gaagagtatg gtggagacgg cacatctgac ggggggatac atgatgtgta
     1981 tcggaaatca cattccgtgg gacattcccc ccgaggcgat taaacgctat ctggatttgt
     2041 cggtggaaat ggcacaccga taggactgcg gcagtcagag cccaagcgcc ttccgaaggt
     2101 gtggctccaa accctgtgcc atccagtaaa agccaagatc gttgggatga acaccatcca
     2161 caaatccatc tgcttgatcc ggtcccaaga gagtgaatcc ttcgacgaga tggatattgc
     2221 gggcacccgc atcctttcgt gcctgcacgg cttttcgaat gacttcgcgc gttctgggaa
     2281 gatccggatg aacatttgaa aagaccgctt cgggtgccat gtagatggct gttgtgcaaa
     2341 tgatcggcgt ttcgggatgt cgggaacgga tgacttcaat aaatgggata tagacttttt
     2401 cgagtgtttc agcatccttt gtgttctggg tgtagtccaa aacgaaacag gatgcgctga
     2461 cttcactgat gatctgagcc acttctggtt ctcccttgcc cgcaccggag aatccaaagt
     2521 tcacaaaatc aacattcaac atccgtccga ggatcgcctg ataactcagc ccgggacgcg
     2581 acgcgcatcc cccctgcgtg atcgatgttc cgtagtagac gaccggcctc tccacggaga
     2641 aatctgatgc cgcatccaac gaagcatcgg gatcaatgcc aatgccctta acagacgcac
     2701 ccatgtagag gggaagataa agagtgatct cgcgttcctt cttttcctga cccttgaaga
     2761 aatgctcctc aacctggatg tcttttcttg gggcaacaga agcgacgtac tgcccgtcta
     2821 cataggcgtc cacaccggac tggccaaagg cgtgcatatt cctcatgttg ggaagagtca
     2881 tatattccac acggatggca agcgatgtcg agtccgtctt gaagcggatg cgtccccccg
     2941 atggataccg ggactggatc catgcaccct caggtaccag cgactgggcg cgctttggaa
     3001 aacgccagaa ctcgccctca ttctccgccc agaaaggcag accattgatc cggaaaacag
     3061 gctctgacac tgaatgccat gacatcgtgg tttcctcctg accgaaagct ttggcgatga
     3121 tcaaggccat gggcagaatg gaaaggagaa agaacagaat gaacagaatc ttggctcgcg
     3181 tgctggatga ggatcgtgac gatcggaagg cagtcttgaa aagggtcgtc ttgttcaaaa
     3241 aaagcagcct ccttggatgg gatgaagaag atcagttctt cttcttgctg tctgttgcat
     3301 gaattccgcc aacttggatg ttcagttcgt cccgatcctt gatggaattc accttgccat
     3361 ccgcgatgta gatgacgcgg tctgaacagg taagcatctt gtggtcgtgc gtggcagtaa
     3421 tcacggtcac gtcacagtct tttgtcaaac ggaagagtat ttcaatgatc tcttgaccgg
     3481 ttgtcaagtc caagttgccg gttggctcat cggcaaggat gatcgacggg tcattggcca
     3541 aggcacgtgc gacggcgact ctctgctgtt ggccaccgga caattctgcc ggtttgtgga
     3601 gcatcctgtc accgagaccg acccgctcaa gcagcgtggc tgccttgtct tgagcatctg
     3661 cttgagtcat gcccgcaaag agcattggga acatcacatt ctcaagagca ctggcaacac
     3721 ggatgagatt gaaggtctga aagatgtagc caatcttgtg acaccgcaac caagccagct
     3781 cgaacgtatc caacgcagcg atgttcacct catcaatgaa aacccgaccg ccctcctccg
     3841 gcttgtcgag tccgccaatc atgttgaaca gcgtgctctt gccggaaccg cttggtccca
     3901 tgatggagac gtattcgccc cgcttgacag agacgctgac gccattgagt gcgtggatag
     3961 tctcctctcc catgcggtag gcttttcgga cgtctaccac tcgaaccaca tgcgcggcca
     4021 gcagcaggtc gcgtgtggga gcagtggaac tgtggaaaat gctgttgggc gccaatccag
     4081 tatccttctc ttccgttctt tcgctattcg aatggaaaca gaaagccaag tggccagccg
     4141 cttaaggcaa gcgttgcttc agcaccaacc gatctctctc caatccaaaa ccaaacaacg
     4201 ggcttgacta tagactcatc acccgtaacc gatcaagcaa aaacaccgat cgggctgtgg
     4261 ccactcagt
//
LOCUS       DAOQUC010000152         4250 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_23274_length_4250_cov_3.119905, whole genome shotgun sequence.
ACCESSION   DAOQUC010000152 DAOQUC010000000
VERSION     DAOQUC010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4250
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23274_length_4250_cov_3.119905"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="PKH07_03855"
     CDS             <1..279
                     /locus_tag="PKH07_03855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34111.1"
                     /translation="DSDGDGYDFTQVDNFTQADLRSLGSVVTRGSDYYRVVSSGEVHG
                     VRKTLTAIVWREYNPNRLTRSEALGLEPTDDEEIPQKVIMTILDYRED"
     gene            372..1952
                     /gene="pilM"
                     /locus_tag="PKH07_03860"
     CDS             372..1952
                     /gene="pilM"
                     /locus_tag="PKH07_03860"
                     /inference="COORDINATES: protein motif:HMM:NF022554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HNT34112.1"
                     /translation="MSSKKKQTLVRNASTTGFELDGRFIRVASLARDAKGNLRLNGCF
                     KKEILLPEDSHDVDALSERVADGLRSLVAEHKLSVGKVISSLAREVVATRNIRLPSQQ
                     TVEIEEMLNYDVERHVPIPASELNLAFDILGYPDELHSQVLLFAAPEKEVSQHLKTME
                     MAGLDPAEIYVDVLGTARSFSEESRNKPLFCVLHLGVHMVNFLVLKEGKLYFARSIPA
                     GQDRLKSFIADETEDLDEKILDFQESDERIRRVQDEWLHLLVQELHRCVHAFENEVYG
                     EKLDHLWISGAMAPCSGLAERLQESLDMPVEIGIPLRGHVLSFNALQDRPEITEYSTS
                     IGLAYCGLDEMPDRLNLLPEEVRQSRRSLEARKLTRNLVSLGAVAVLLFAIAFGIKWQ
                     ERYSEVRHLRKLEALLTPRIEQLKEVERELGLIDERMDRQNSALKVIRNLYEILPEKV
                     CVAKMDFLKKKHVQLTIQTFTDQERDQVISILRESPHLRDAKFEGSQPRNVYGVTLTE
                     AKVTCPLESAGRRTTTRK"
     gene            1998..2597
                     /locus_tag="PKH07_03865"
     CDS             1998..2597
                     /locus_tag="PKH07_03865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34113.1"
                     /translation="MKLLRTRRERVLFGLTMVVILGTVGYFVLYQRLSDLWVSLGDER
                     DMLEMKIDQMRVLIEKNRQLESRCLTMKSYLEIPGSAEEQQSEVLKEITSILDQSGIV
                     ATTKPLAWTDDKNFRVFVFQFSNINCPLDSLLVLLKSIDEESSVLEVEEISISSVFMQ
                     GGIAKVKADMKLSRLVYSENREGADRTGQSASATSPKGA"
     gene            2604..3113
                     /locus_tag="PKH07_03870"
     CDS             2604..3113
                     /locus_tag="PKH07_03870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34114.1"
                     /translation="MKDNGHEAFRQGTYALMWGLIMAGGTFLTLIAMSVYAQPRRDYI
                     QVEDLAPLLTPAAPLDASVPETGPDLVVFSQRNIFEEIITPVPTATKVPKPSPSPTQR
                     MPGLHWKLKMVAGNVCGIEDFQKQFKMLELGEEYQGARVKEINMDDQSVVIEDILDGN
                     TRTLRMSAE"
     gene            3215..>4250
                     /locus_tag="PKH07_03875"
     CDS             3215..>4250
                     /locus_tag="PKH07_03875"
                     /inference="COORDINATES: protein motif:HMM:NF015891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HNT34115.1"
                     /translation="MKTVLVLCLVLLLGQTTAFSQESKTDNDSQRFLMMTPVARPLDA
                     TLLATLIPELQADQWTPKQFFAALSEKVGNPIAISSEILRENQRISAGYRSKTFKNIM
                     DHICVDRKWIYDFEHVLETDVEHRRATLNILMIRVHTAKEDPPMGTLRAIKSIRLINV
                     SPDDALQAALKAKSPTGIVYMSTSSPHILVEDDPEWIGKVKQAIVDADEDEVTRVVSV
                     TKRTPEEIVAEIEKLLPDQEGQLIPDAIGGKIYLQDTQEMADKLEKLIHELDSGAPVG
                     TVRPTSRTVRIRNAAAEQVAADLLRLMPELTGIVKADLTENTITITGDPSRWDALERL
                     IQGLDDQTQSK"
BASE COUNT         1031 a         1035 c         1218 g          966 t
ORIGIN      
        1 gacagcgacg gggacggata tgatttcaca caggtggata atttcaccca ggcggatctc
       61 cgcagtctcg ggtcggttgt gacgcggggt tcggactatt accgcgtcgt gagttccggt
      121 gaagtgcatg gagtgcgaaa aacgctgaca gcaattgtct ggcgtgagta taatcccaac
      181 cgcctgactc gatccgaggc gttgggactg gaaccgacgg acgacgagga gattccgcag
      241 aaggtcatca tgaccattct ggactatcga gaggactaga tttagagcga atctgatttc
      301 tgccgaaagt gatcatcgct tcactgcgag gcaacgcttc cggcaaaacc accagaggga
      361 gacagactgg cttgagcagc aagaagaaac agacactggt caggaatgct tcaacgaccg
      421 gctttgaact ggacggacgc ttcatccgtg tggcgagtct ggctcgcgat gccaaaggga
      481 atcttcggct gaacggctgt ttcaagaagg agattctcct tcctgaggac tcccatgatg
      541 tggatgcttt gtccgagcga gttgcagatg gactgaggtc tctcgttgca gaacacaaac
      601 tctctgtggg gaaagtgatt tcctcgcttg cacgggaagt tgttgccaca cgcaacattc
      661 ggcttccttc acagcagact gtggaaatcg aagagatgct caactatgac gtggagaggc
      721 atgtgcctat tcccgcttcg gaactcaatc tggcctttga catcctgggc taccccgatg
      781 agctgcactc tcaagtgctt ctgtttgccg cgccggagaa ggaagtgtcg cagcatctca
      841 agaccatgga gatggctggg ttggatccag ccgagatcta tgttgatgtg ctgggaacgg
      901 cgcgctcttt cagtgaggag tctcggaata agcccctctt ctgtgttctg cacctgggtg
      961 ttcacatggt caacttcctg gttctcaagg aaggcaaact ctacttcgcg agaagcatcc
     1021 ctgccggtca ggatcgcctg aagtcgttca tcgctgatga aaccgaggat ctggatgaga
     1081 agatcctaga ctttcaggaa tctgacgagc ggatccgaag agttcaggac gaatggctac
     1141 acctgctggt gcaggaactg caccgatgcg ttcacgcgtt tgaaaatgaa gtctacgggg
     1201 agaaactgga tcatctgtgg atcagtggtg ccatggcacc ctgttcgggg cttgccgaga
     1261 gacttcagga atcactcgat atgcccgtgg agatcggcat tcctctgaga ggacatgttc
     1321 tgtcgttcaa cgccttgcag gacaggccgg agatcactga gtactcgacc agcattgggc
     1381 tggcctattg cggcttggat gagatgcctg accgactgaa tcttttgccg gaggaagtac
     1441 ggcagagtcg tcgctcattg gaagcaagga aactcacccg aaacctggtt tctttgggtg
     1501 cggtcgcagt gctcttgttt gcgattgcct tcgggatcaa atggcaggag cgctattccg
     1561 aggtgcgtca tttgagaaaa ctggaagcgc tcctcacacc ccgaattgag cagctcaaag
     1621 aagtcgagcg cgaactgggg ctcattgatg aacgtatgga tcgtcagaat tcggcgctga
     1681 aggtcatcag aaatctctat gagattcttc cggaaaaagt ctgcgtggcg aaaatggact
     1741 ttcttaagaa aaagcacgtt cagttgacca tccagacatt tactgatcag gaacgggatc
     1801 aggtgatctc tattctccga gagtctcccc atctacggga tgccaagttc gagggttccc
     1861 agccgagaaa cgtctatggg gtgacgctta cggaagccaa agtgacctgt ccgttggaat
     1921 cggcaggcag gagaaccacg actcggaaat gactgtccgg gtcacataac gaagatacct
     1981 gctgacagga gcatgcgatg aaacttttgc ggacaaggcg tgaacgagtg ctgttcggcc
     2041 tgacgatggt tgtgattctc ggcacagtgg gctactttgt gctctatcaa cgtctgagcg
     2101 atctgtgggt ttccctgggg gatgaacggg acatgctgga gatgaagatc gatcagatga
     2161 gagttcttat cgagaagaac cggcagctgg agtcccgatg tctgacgatg aagagttatc
     2221 ttgaaattcc aggaagtgcg gaggaacagc aaagcgaggt actcaaggag atcacctcta
     2281 ttcttgacca aagtggcatc gtggcgacga ccaagccact tgcctggacc gacgacaaga
     2341 acttccgggt tttcgttttt cagttcagca acatcaactg ccctctggat tccctgttgg
     2401 tgcttttgaa aagcattgat gaggaatctt ccgtgctgga agttgaagag atctccatct
     2461 cctcagtctt catgcagggg ggcatagcca aggtcaaggc agacatgaaa ctctcgcgac
     2521 ttgtctacag tgagaatcgc gaaggcgccg acagaaccgg tcagagcgca tcggcgacgt
     2581 cgccgaaagg agcgtaacag ccaatgaaag acaatggtca tgaagcattt cggcagggca
     2641 cctatgcact gatgtggggt ctcatcatgg caggtggcac gtttttgact cttatcgcaa
     2701 tgagtgtcta tgcccaaccc cgccgcgatt acattcaggt ggaggacttg gctccactgc
     2761 tgactcctgc ggcacccctg gatgccagtg tccctgagac aggtcccgat ctcgttgttt
     2821 tttcgcagcg gaacattttc gaggagatta tcacaccggt tcccactgca accaaggttc
     2881 ccaagccatc accctcgcct acacagcgaa tgccaggact ccactggaag ctcaagatgg
     2941 tggccgggaa tgtgtgtggc atcgaagatt ttcagaagca gttcaaaatg cttgagttgg
     3001 gtgaggagta tcaaggtgcc agagtcaagg aaatcaacat ggatgatcag agtgttgtca
     3061 ttgaggatat tcttgatggc aataccagaa cgctgagaat gtcggcggag tgacgggtag
     3121 caggatgctg tcaaattccg cagttcatat tctgaaaaca gagagagttc ccatgaggct
     3181 cttgcggccg agtccatgtt ttggagcgag gacgatgaag acagtccttg tgctgtgttt
     3241 ggtcttgctt ttgggacaaa cgactgcatt ttctcaggag tccaagaccg acaacgactc
     3301 acagcgtttt ctcatgatga cgcctgtagc acggccattg gatgccactc ttcttgccac
     3361 cctgattccg gagctgcaag ccgatcaatg gacgccgaag cagttctttg ccgccctttc
     3421 tgagaaggtg ggcaacccaa ttgccatctc ctctgagatt ctcagagaga accagaggat
     3481 ctctgcgggc tatcggagca agaccttcaa gaacatcatg gatcacatct gcgtcgatcg
     3541 aaagtggata tacgattttg agcatgtcct tgaaaccgat gtggaacacc gtcgagcaac
     3601 gttgaacata ctcatgattc gggtgcacac ggcgaaagaa gatcccccta tgggcacttt
     3661 gcgcgcgatc aagtccattc ggctcatcaa cgtttctcct gacgatgcgc ttcaggctgc
     3721 actgaaggcg aagtcaccca ccggtattgt ctacatgagt acttcttcgc cacacattct
     3781 cgttgaggac gatccggaat ggatcgggaa ggtgaagcag gccattgtgg atgccgatga
     3841 ggatgaagtg acacgagtgg tttccgtgac caaacggact cctgaggaaa tcgttgctga
     3901 gatcgagaaa ctgcttccgg atcaggaagg ccagctcatt cctgacgcga ttgggggcaa
     3961 gatatacctc caggatactc aggaaatggc tgacaagttg gagaaactga tccacgagtt
     4021 ggattcggga gcgccggtag gtacggttcg acccacgagt cggactgttc gaatccggaa
     4081 cgctgcagcc gagcaggttg ccgctgatct tctgcgactg atgcctgaac tgacgggtat
     4141 cgtgaaggca gatctgacag agaacacgat cacgatcacg ggcgatccct cccgttggga
     4201 cgctttggaa cgactgattc agggtctgga cgaccagacc cagagcaagg
//
LOCUS       DAOQUC010000153         4240 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_23366_length_4240_cov_3.407646, whole genome shotgun sequence.
ACCESSION   DAOQUC010000153 DAOQUC010000000
VERSION     DAOQUC010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4240
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23366_length_4240_cov_3.407646"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1382)
                     /locus_tag="PKH07_03880"
     CDS             complement(<1..1382)
                     /locus_tag="PKH07_03880"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HNT34116.1"
                     /translation="MKNSGDPVATKDLSRARCSSPCLSGKGCQQEGARPSRRRRILIA
                     ATVIPALCVASLCLLFSSVSVVPDDLRISDDLILTDRDGRVLRHVPGLREERHLWTNL
                     AEVPPLLRQAFLAAEDRRFFEHHGVDPRAMLRAFLSNCRSRRIVSGASTITQQLVRIT
                     YPRQRTYAAKLVEIVRAIKVERRLKKETLLEYYLNRVPMGNNIVGVGLASQVYFGRTP
                     EHLDAASCALLASLPKAPATLNPYGKDTERLKTRRNWILGRMLQQGWLSKEEHDRAVL
                     REIEVRPKSFAFNAPHFVDWVIAKSAQSKESALFGVHRTTLDLEMQRNLEKVLASHQQ
                     RLLANGCRQAGAIIVHNPTMEVLALSGSLNYSQKDGGFNNVVTAMRCPGSTLKPFLYA
                     HALDSGFTVSTLLGDTERGYRSPTGSYFPRNFDRYEYGPVTLRTALGSSLNLSSVQML
                     QMVGQDSFFE"
     gene            complement(1379..>4240)
                     /locus_tag="PKH07_03885"
     CDS             complement(1379..>4240)
                     /locus_tag="PKH07_03885"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HNT34117.1"
                     /translation="QCTYRDSDGNDYSSGTIYDVEGPYFWYEEEERVRPYERIQLLPD
                     KKEYAIGDTIRILLKSTKPLTSCLLTVERDKVLEHRLIEVRNNEISIPVSKEFYPNAY
                     ISLTGMIPRGDFPTYQGEYDTDSPNFACGTLNVKIRNEVQSLKVQILDGADIPKSLPG
                     QRMTLDFQVTDESNKAVTAELAVGVVDEQVLALTRYKTPDLSGLADFLLPLKLLTGEN
                     RTALQMQTPFKPLRNEPLSGGDGAYGDGRRDPLDAKLRKDFRPVAYFNPAVQTDDSGK
                     ASVTFEFPDSMTTYRVYVVACDAGTRFASVEKSALVVKDFYLEPGLPSFLIKGDQFQS
                     QVAAFNKTDGSGAMSFDTKSSEQLAIESPSRSYALSAQDRVLVPLTGAATSVGTATVQ
                     LSAGFESKQDAVEIKLPVHSGYQRHRDVAFGVVHSGAGIEYTFPEILSQLPLDEIEPE
                     DFTVELTLSKSPFLRMKPGLQYLLKYPYGCIEQTSSGVIPLAALRGLIRDGLVPGITI
                     EEADKFLSKGVERLLSMQLNSGGFSYWPRQGQPTPWGSIYATTALLFAREAGFAVPDE
                     QYQSALNYLVERMDEDSSNLDWGDTSFSGIVAFILAKSDRLSRGEWEKLQKQSSKIGL
                     QGRMLLVLAGKQNGTLSEKEALSKFREILNEPPSDRRGCAYYAKHREDAIALYCCNEI
                     IAAGTEAAMYSEKLLKSMNPDGRWSSTSDTGWALMALGNYFKGMNLAQGSVKGLARAR
                     GMQEGPFELDGADSITMSINAAEFLEQPSVDIETERDASLFYELVVVYPRVDYARNGY
                     SGGFTLSKSIKNTDGSEDIRVGDVVEISITLWGKGQDLRYLVLDDPLPAGFVAINPAL
                     ANEEPMRPTSSRDQDFYWRYWDLAGYYKLVPNHLEIRDDRVLVFRDNLWSGEYRYSYH
                     ARAVCAGTFKIPSTMLQLMYETDVCAYTPETMITVKERR"
BASE COUNT          972 a         1154 c         1107 g         1007 t
ORIGIN      
        1 aactcaaaga aggaatcctg accgaccatc tgaagcatct gaacgctgga gaggttcagg
       61 gagctgccca gagcagttct gagcgtgaca gggccatact catatcggtc gaaattcctt
      121 gggaaataac tgccagtcgg agatcggtaa ccccgttctg tatctcccaa caaagtcgag
      181 acagtgaatc ccgaatccaa ggcatgggcg tagaggaatg gtttcaacgt ggaacctgga
      241 caacgcattg ctgtcacaac attgttgaat cccccgtctt tttgcgagta attcaaagaa
      301 ccggagagcg ccaagacttc cattgtcggg ttgtgtacta tgatcgctcc ggcctggcgg
      361 caaccattgg ccagcaggcg ctgttggtgt gaagcgagaa ccttctccag atttctctgc
      421 atctccagat cgagcgtggt tcgatgaacg ccaaacagcg cagattcctt cgactgtgcc
      481 gacttggcga tcacccagtc aacgaaatga ggtgcgttga aggcaaatga ttttgggcgc
      541 acttcaattt ctcgcagtac cgcccgatca tgttcttctt tcgaaagcca accctgttgc
      601 agcattcgac ccagaatcca gttgcgcctt gtcttgagcc tttcggtatc ctttccatag
      661 ggattcaatg tggcaggtgc tttcggcagc gatgccagca atgcacatga ggcggcatcc
      721 aagtgttcgg gtgttcgccc gaaatagacc tgcgaagcca agcccactcc gacgatgttg
      781 tttcccatcg gcacgcggtt gagataatac tcaagcagag tttctttctt gaggcgtctc
      841 tcaactttga tggcacggac gatctcgaca agttttgccg cgtatgttct ctgcctcgga
      901 taggtaattc gaaccagttg ctgtgttatc gttgacgcac cggatacaat cctcctggag
      961 cggcagttgg agaggaaagc gcggagcatg gctcttggat caacgccatg atgttcgaaa
     1021 aagcgcctgt cttctgctgc cagaaaagcc tgacgcagaa gtggtggcac ctctgccaga
     1081 ttcgtccaga gatgtctttc ctcacgcaga ccaggaacat gccgaagcac tcgcccatct
     1141 ctgtccgtga gaatcaggtc gtcggaaatc ctcagatcgt caggcaccac ggagaccgat
     1201 gagaacaaca gacaaaggga cgcaacacac aacgcgggta ttaccgtcgc cgcgatgaga
     1261 attctccggc ggcgagatgg gcgtgcacct tcctgttggc agcccttgcc cgaaaggcaa
     1321 ggactggagc atcgggcgcg ggataaatcc ttcgtcgcga caggatctcc tgagttcttc
     1381 atcgtcgctc cttgacggta atcatcgttt cgggagtgta ggcgcacaca tccgtttcat
     1441 acatgagttg gagcatcgtt gagggtatct tgaaggtgcc agcacagacg gcacgtgcat
     1501 gataactgta acgatattcg ccactccaca ggttgtctcg gaaaacaagc actcggtcat
     1561 cacggatttc cagatgattg ggcacgagtt tgtagtagcc tgccaaatcc caatatctcc
     1621 agtagaaatc ctgatctcga gaagatgtag gacgcatcgg ttcttcattc gccagagcag
     1681 gattgatggc aacaaagcct gccggcaaag gatcgtcaag aaccagatag cggagatcct
     1741 gcccctttcc ccatagagtg atgcttatct caactacgtc tccgacgcgg atatcctcac
     1801 tgccgtccgt gttcttaatg ctcttgctca gagtgaatcc accggagtag ccgtttcggg
     1861 catagtcaac tcttggatag acgacaacca gctcatagaa tagcgaagcg tccctttcgg
     1921 tctcaatgtc aacgcttggt tgttcgagga attccgcggc attgatggac attgttatag
     1981 agtctgcacc atcgagttcg aaggggcctt cctgcatacc tctggctcgt gccagtcctt
     2041 taacgcttcc ttgagcaagg ttcattccct tgaagtagtt ccccaaggcc ataagcgccc
     2101 aaccggtgtc gctggtcgaa ctccatctgc cgtcaggatt catggatttc aggagtttct
     2161 cggaatacat ggccgcttcg gtacctgcgg cgatgatttc attgcagcag tagagtgcga
     2221 tggcgtcttc ccgatgcttg gcgtagtagg cacagcctcg tcgatcagaa ggcggttcgt
     2281 tcaggatttc gcgaaactta gagagtgcct ccttctctga gagtgtccca ttctgcttgc
     2341 ccgcaagaac caggagcata cgtccctgca atccaatctt gctggactgt ttctggagtt
     2401 tttcccattc gcccctgctg agtcggtcag actttgccag aatgaacgcc acaattccgc
     2461 tgaatgaagt atcgccccag tccagattgc tcgaatcttc gtccattctc tcaaccagat
     2521 aattcagagc cgactggtat tgctcgtccg gcacagcgaa acctgcttct cgagcaaaca
     2581 agagcgctgt ggtggcgtaa atactgcccc agggcgtcgg ttgcccctga cgcggccaat
     2641 aactgaaacc acccgagttc aactgcatgg agagcagtcg ttcgacccct ttgctgagaa
     2701 acttgtcggc ttcctcaatc gtaatgcctg gaacaagacc gtcgcggatc aacccgcgca
     2761 gtgccgcaag tggaatcact cccgaactgg tttgctcgat acagccgtag ggatatttga
     2821 gcaaatactg aaggccaggc ttcatccgaa gaaatgggga tttggagagt gtcagctcga
     2881 cggtgaaatc ttccggttcg atttcgtcca acggcaactg tgacaggatt tcggggaagg
     2941 tatactcgat acccgctccc gaatgaacca cgccaaaagc gacatcacga tgtcgttgat
     3001 atcccgaatg cactggcagt ttgatctcca ccgcgtcctg tttggattca aatccagcgc
     3061 tcaattgaac ggtggcggtt ccgacagatg ttgcggctcc ggtcagcgga accagcactc
     3121 tgtcctgggc actgagcgca taggatcggg aaggactctc gatcgcaagt tgttcgctgc
     3181 tcttggtgtc aaaggacatg gcacctgatc cgtcagtctt gttaaaggcg gccacttgtg
     3241 attggaactg atcacccttg atcaagaacg aaggcaagcc gggttcgaga tagaaatcct
     3301 tcacgacgag agcgctcttc tccacgctcg caaaacgcgt tccggcatca cacgccacga
     3361 catagacccg ataggttgtc atggagtccg ggaattcgaa cgttaccgat gccttgccgg
     3421 agtcatctgt ctgaaccgct ggattgaaat aggcaaccgg tcggaagtcc tttctcagct
     3481 tggcatccag aggatcacgc cgcccatccc catacgcgcc atcaccgccg gacagaggct
     3541 cattgcgcaa cggcttgaac ggtgtctgca tctgaagtgc ggttcggttc tcacccgtca
     3601 gcagtttcag aggcaagaga aaatcggcca gaccactcag gtccggagtc ttgtatcgag
     3661 tcagcgccag aacctgttca tccacaacac ccacagcaag ctccgccgtc accgccttgt
     3721 tcgattcatc ggtgacttga aagtccaggg tcatcctctg tcctggaaga ctcttgggaa
     3781 tgtccgcgcc gtccagtatc tgcactttca gactctgaac ttcgttacga atcttgacgt
     3841 tcaaggttcc gcaggcgaag ttcggagagt cggtgtcata ctctccttga taggttggga
     3901 aatccccacg tgggatcatc ccagtcaagg aaatgtaggc gttcggatag aactccttgc
     3961 tgacgggaat ggaaatctcg ttgtttcgaa cctcgatcaa ccgatgctcc aagaccttgt
     4021 cgcgctccac cgtcagcaga caactcgtca gcggcttggt gctcttgagt agaattcgga
     4081 tggtgtcccc aatggcatat tccttcttgt cgggtagcag ttggattcgt tcgtaggggc
     4141 gaactcgttc ctcttcttca taccagaagt agggtccttc cacgtcatag atggttcctg
     4201 atgagtagtc gttgccatcc gaatcgcgat aggtgcattg
//
LOCUS       DAOQUC010000154         4236 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_23402_length_4236_cov_3.156900, whole genome shotgun sequence.
ACCESSION   DAOQUC010000154 DAOQUC010000000
VERSION     DAOQUC010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4236)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4236)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4236
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23402_length_4236_cov_3.156900"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3836
                     /locus_tag="PKH07_03890"
     CDS             <1..3836
                     /locus_tag="PKH07_03890"
                     /inference="COORDINATES: protein motif:HMM:NF024371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HNT34118.1"
                     /translation="VLLLISSQVAASPKSSFADRGWREYFSGDIRLAQKIWERSLRGR
                     GRSPLSWLGLSLIEESHGDLPSAYRHISSAIACLVEEPRLKPHEYIVPLEVYAIHLCS
                     LAPFADGVESAQRTLSMVLDRSDLPFETRAQVTYLLVSLLRSQGRLEEAEALCDSMGF
                     IRDFLVIGPFDSPEQSGWQTAYPPETEFIASGEYQGKRGFLSWSPVSVAPKWGYVNLA
                     NQLRPQQDATAYLSCFVRCPTETPVRLWMGYGGAAKLWVNRRLILADSEYHPFWMDQL
                     SADAVLSEGTNQILVKVCSDEDRGWGVMLRITDPQGDPHVAFDLTTAPDEFTCAASEA
                     QTLSASDDRVGELPVVLRDDVDESSDPVERALRHLSRAYLYRLWNLYHRGLSPDLEEV
                     RKAESLYSSNPLIEKMLASLEKDNSRRSHALQKAIQSDPYDVEAMMLLVSLYQNELID
                     EAADSLISTLSSLARDYAPGRIVCGNDLERRGSSLLARQAYLDAANSAPNWYRSWYLL
                     GMQTSNLMSRMEALPFLERAVLCDKSLSEPARALFDLHRELGNQEEAESLANASLEQR
                     PDDLRLALDLGRWYAAGEEHGKAEQWLNRAMSISPNDPECNTEMGHLRERRGEMTEAI
                     RYWSHALQIRPNDAQLQEYLAHNLSEGADYYAPFELQLQSLDEPDIHLEEANIVCLAD
                     QQVRRVYPDGTSRRTVHQVNRVVNSAGIRQAAFEAIYYVPSREDVVVHTARVITKTGQ
                     QIFAPEPQVRSALDLSGVGSGIYSDYDVMIVSFDGVEKDALVELKYEIRENQENLYAD
                     YFGDIFYFGSYDPTVLSEYVLIFPKHRKLHHIFVPNGSEQPDNLSVQEEGDTCVYRWH
                     YKNLPSIRREPMMPAVSEMLPYLKVSTFSTWDEMASWYWDLSRESLRPDTTVSATARQ
                     LITDPHATASQKLNAVFRYVSNEIRYLGLEFGVHGYKPHGAGHVNRMQYGDCKDKAGL
                     AISLLKELGVPAQLVLLRTVDKGRIDLSLPSLGLFNHAIFRASLSDLTAEWFDGTADY
                     TALGELPSMDQGAQVLVIHEGGRFTLEQIPISPPSANLIKYRTELVLSPEGNASGRRR
                     ASFHGLFNRSIRAAYQNDAKNPQIIEQQFGSAFPGSLITDVLHSDFSALSPEEYIEFS
                     LELPRFAHSQNGALRFSACLFPERLTEAYAPLTTRRHQLVNHGLWQKERTLTLTLPPG
                     SVCEEIPKDFHLDTPFGEFKRTYRHGNSKIIYEETLVFKAQRVGSQDYGAFRGFCASV
                     DREQKSEVVLRPDRQ"
     gene            3847..4230
                     /locus_tag="PKH07_03895"
     CDS             3847..4230
                     /locus_tag="PKH07_03895"
                     /inference="COORDINATES: protein motif:HMM:NF009154.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HNT34119.1"
                     /translation="METPHKQESTRKKGSRYEDLAEAYLSEKGYRILERNIHSRYGEI
                     DIVAEDGSVIVFVEVRMRSRNDYGSPAESITPSKMRKILREAEMYLVENSLLDRDARL
                     DVISILDRGQGTPEITHLTNVSEER"
BASE COUNT         1008 a         1095 c         1116 g         1017 t
ORIGIN      
        1 tggttctgct gttgatctcc agtcaggttg ccgcctcacc taagtcgtct tttgcagacc
       61 gtggatggcg cgagtatttc agtggcgata ttcggttggc gcagaagatc tgggaaaggt
      121 ccctcagagg tcgtggcagg tctcctcttt cttggctcgg actttcacta atcgaggagt
      181 cccacggaga tcttccaagc gcatacagac atatttccag cgctattgcg tgtcttgtcg
      241 aagagccaag gcttaagcct cacgagtaca tcgtgccctt ggaagtctat gccattcatt
      301 tgtgctccct tgctccgttt gcagatggcg tggagtcggc acagaggact ctgtctatgg
      361 tacttgatcg ttcagaccta cctttcgaaa ccagagcgca ggttacatat cttctcgtat
      421 ctttgcttcg gagccaaggt cggttggagg aagctgaggc tctctgtgat tcgatgggtt
      481 tcatcagaga ctttctcgtc attgggcctt ttgatagtcc ggagcaatcg ggttggcaga
      541 cagcctatcc ccctgaaact gagttcattg catctggaga gtatcaagga aagagaggct
      601 ttctttcttg gtctcctgtg agtgttgctc caaagtgggg atatgtgaac cttgccaacc
      661 agcttcgtcc gcagcaggac gcaacagcct atctgtcttg cttcgtccga tgccccacag
      721 aaacaccagt gcgcttgtgg atgggttacg ggggagctgc taagttgtgg gtcaacaggc
      781 gattgatcct cgctgattca gaatatcatc cgttctggat ggatcagttg agtgctgacg
      841 cagtgctttc cgaaggaacg aaccagatac tcgtcaaagt ctgctccgat gaagaccgag
      901 gctggggagt aatgcttcgt atcaccgatc cacaaggtga ccctcatgtc gcctttgatc
      961 tgacaacggc tcccgatgag ttcacctgtg ctgcttctga ggcacagact ctttcggcat
     1021 ctgatgatcg cgtcggggaa ctgcccgttg tgcttcgcga tgatgttgat gagtcgtcgg
     1081 acccagtgga gcgtgccctc cgacacctct ccagagccta tctctaccga ctctggaatc
     1141 tctatcacag agggctttct ccggatttgg aggaggttcg caaggcggaa tccctatata
     1201 gctcaaatcc tcttatcgag aagatgctcg catccctgga gaaggacaat agtcgaagga
     1261 gtcatgctct tcaaaaggct atccaatctg acccgtatga tgtagaggcc atgatgcttc
     1321 tcgtctccct gtaccagaat gaactgatcg acgaggccgc cgacagcctc atcagcactt
     1381 tgtcatcgct ggccagggac tatgctccag gccggatcgt ttgtggaaac gatcttgaga
     1441 gaagaggctc cagtcttctt gctcgacagg catatctcga cgccgcgaac agcgccccaa
     1501 attggtacag atcctggtat ctgctcggga tgcagacctc aaaccttatg tcaaggatgg
     1561 aggcccttcc ctttctcgaa agggctgtct tgtgtgacaa gtctctctcg gaacctgcca
     1621 gagcgctttt tgatctgcat agagagttgg ggaatcagga agaggcggag agcctcgcga
     1681 acgcatctct ggagcagaga ccggatgatc tgaggctggc cctggatctc gggcgatggt
     1741 atgcagcagg ggaggaacat gggaaggctg aacaatggct gaatcgcgca atgagcattt
     1801 ctccgaacga tcctgagtgc aatactgaga tggggcatct ccgtgagcgg agaggagaga
     1861 tgactgaggc aatacggtac tggagtcatg cactgcaaat ccgacccaat gacgcacaac
     1921 tccaggagta tcttgcccat aacctctcag aaggtgcgga ctactacgct ccctttgaac
     1981 tgcagttgca atctcttgat gagccggata ttcaccttga ggaagccaac attgtctgcc
     2041 ttgctgatca acaagttcgt cgcgtctatc cggatggtac atcaagacgc acagtgcatc
     2101 aggtgaatcg cgtcgtcaac tcagcaggaa tccgccaggc cgcgtttgag gccatctact
     2161 acgttccttc aagagaggac gtcgtagtcc acaccgccag ggttatcacc aagacaggac
     2221 agcagatatt tgcccctgag ccacaagtcc gatctgcgct tgatttgtca ggtgttggga
     2281 gtggcatata cagcgactac gatgtcatga tagtcagttt cgatggcgtg gaaaaggatg
     2341 ctctggttga gctgaagtat gaaattcgcg aaaaccagga gaatctctat gccgactact
     2401 ttggagacat tttttacttt ggctcttatg atcccaccgt actcagtgaa tacgttctca
     2461 tcttcccaaa acaccggaag cttcatcaca tcttcgtccc aaatgggagt gaacaacctg
     2521 acaatctttc tgtgcaagag gaaggggaca catgcgtcta ccggtggcac tacaagaacc
     2581 tgcccagcat tcgccgcgag ccaatgatgc cagcggtctc agagatgctc ccctatctca
     2641 aagtctcgac attctcaaca tgggatgaaa tggcttcatg gtactgggat ttgagccgtg
     2701 aatcactgag gccggatacc acagtgagcg caacagcacg ccagttgatc acagaccctc
     2761 atgctactgc aagccaaaaa ctgaacgccg ttttcagata tgtcagtaac gagattcggt
     2821 atctggggct ggaatttggc gtccacggct acaaacccca tggtgcaggc cacgtcaatc
     2881 gaatgcagta cggtgactgt aaggacaaag ctggactggc catttcgctc ctgaaggaac
     2941 tcggagtccc ggcacagttg gttctgctgc gaacggtaga caagggacgc atagacctta
     3001 gcctgccgtc gcttgggctt ttcaaccatg ctatcttccg cgcgtctctt tcggatctca
     3061 ctgccgagtg gtttgatggc actgcagatt acacagcgct cggtgaactt ccatctatgg
     3121 atcaaggggc acaagttctt gtgatccacg aaggcggaag gttcaccctt gaacagatcc
     3181 cgatctctcc tccctctgcg aatctcatca agtataggac ggaactcgtt ctctctcccg
     3241 aaggaaacgc atcaggaagg cgcagggctt cgttccacgg cctgttcaat cgctccattc
     3301 gagccgctta tcaaaatgat gccaagaacc cacagattat tgaacagcag ttcggaagcg
     3361 cctttcctgg ttcactcatc accgacgtac tgcattctga cttcagtgcc ctgtcacccg
     3421 aggagtatat tgagttttcg cttgagttgc cacgatttgc acactctcag aatggtgcgc
     3481 tacgtttctc tgcttgtctc tttcccgaga ggctcacgga agcatacgct cctttgacca
     3541 cgcgcaggca tcaacttgtt aatcacggac tttggcagaa ggaacgcact ctcactctga
     3601 cgttgcctcc cggctctgta tgtgaggaaa tccccaaaga ctttcatctg gatacaccct
     3661 ttggagaatt caagcgaacc tatcgccatg gcaacagcaa gatcatctac gaggagactc
     3721 ttgtcttcaa ggctcagaga gttggttcgc aagactacgg agcgtttaga ggattttgcg
     3781 cttccgtaga ccgagagcag aagagtgagg ttgttctgag gcccgatagg cagtgagcat
     3841 tctgtgatgg aaacacccca caagcaagag tcaacacgaa agaagggatc ccgctacgag
     3901 gatctcgcgg aagcctatct ttctgaaaag ggctatcgga tcctcgaaag gaacatccat
     3961 agcagatacg gggagataga cattgtggca gaggatggtt ctgtcattgt cttcgttgag
     4021 gtgcggatgc gctccagaaa tgactatgga agtccggcag aatcgatcac cccttcaaaa
     4081 atgaggaaga ttctgcgaga ggcggaaatg tacctcgtcg agaatagcct gctcgataga
     4141 gatgctcgcc tagatgtcat cagcatcctg gacagaggac agggaacacc ggagatcacc
     4201 cacctgacaa acgtgtccga agaacgatag actcta
//
LOCUS       DAOQUC010000155         4227 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_23470_length_4227_cov_3.742570, whole genome shotgun sequence.
ACCESSION   DAOQUC010000155 DAOQUC010000000
VERSION     DAOQUC010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4227
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23470_length_4227_cov_3.742570"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..621)
                     /locus_tag="PKH07_03900"
     CDS             complement(<1..621)
                     /locus_tag="PKH07_03900"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNT34120.1"
                     /translation="MSSQNSPESALEIRGLEKTFYTFHLGPLDLTVPVGAVYGLIGPN
                     GAGKTTTIDLVVGLGRNDSGTIKVLGFDHIRDEVELKKRVGYVSPDLDFLAWRTVGAL
                     ISFICAFYPEWDFDYCENLLARFHLLRDAKISELSLGERTKLALTLVLPRRPPLLLLD
                     EPTIGLDAISKKEFFAEILKLVEDGHHSVLISSHGLSDIERFADHIG"
     gene            complement(639..1040)
                     /locus_tag="PKH07_03905"
     CDS             complement(639..1040)
                     /locus_tag="PKH07_03905"
                     /inference="COORDINATES: protein motif:HMM:NF012610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HNT34121.1"
                     /translation="MKDATKRILLHPITSAVAKPLYQQVVDALKTEITEGRLKSGELL
                     PSFRQLAEQLMVSLITVKRAYEELERQGIIYRRQGMGTFVSEDGQDRNQMIKRENAQT
                     LMRDALKEALESGLSRDEIRQMFERLEKEQF"
     gene            complement(1487..1897)
                     /locus_tag="PKH07_03910"
     CDS             complement(1487..1897)
                     /locus_tag="PKH07_03910"
                     /inference="COORDINATES: protein motif:HMM:NF017020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HNT34122.1"
                     /translation="MARPSTKQVEQARLLLDKAAEDEHLLDVVMDDVSVADAVYGFHC
                     QQAAEKMLKALLSAHGASFPRTHDLTELLNLLTEAGISLPEEFTWLDSLTPFAVELRY
                     DLVGYEGQIDRRAIRETLHNLRTLVLKAIQGKPD"
     gene            complement(1869..2213)
                     /locus_tag="PKH07_03915"
     CDS             complement(1869..2213)
                     /locus_tag="PKH07_03915"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HNT34123.1"
                     /translation="MNDKTQPIWAVTEEKIREAVQRIAEIANPMEIICFGSQARGNAN
                     NNSDLDLMVVLEHVSDKVQESVRLRRALSGLIMGVDILVVDKARFDYWRDTPGNVYYE
                     AVQDGKTVYQAG"
     gene            complement(2453..>4227)
                     /locus_tag="PKH07_03920"
     CDS             complement(2453..>4227)
                     /locus_tag="PKH07_03920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34124.1"
                     /translation="PIPVGRFLCVKYFGGLVALLIIFSIPLLLETLTSNYGREREWYL
                     WFSSYTANIAWTTPWLVLMVYSVAFLIGCVNRSTVTTALLSLGAGLLIVFAPLLLRPI
                     SFMSLFEWMVNPPIHLWPVAKHYLSTRSRYFFVLFEYGVYIEKGYVLFAICSLLISAA
                     ALFLSIVTVKKNITIDARQRTICWSLVAVVLLLSLGVSTELGTNMESDVVSLPEYCNF
                     ISSLHQFGDRTFALMQPGIINEHFQLAEIDVSGKATFSKRIWMPDRFWKEGDQSKPAV
                     SQWIKRPVAWSPNQPNRVYTFIDHCEPEETLRYGREFFNYKRQRLELIALDLDRAREP
                     LPDNRHEDIDPVLASIDMSAYLKISEDQGITTYNDASWPLFPEMSAYGNHLFILHRGR
                     SVLLFDISGELGIPRFIAEQPADKIFTHMLSMGQGNEWILPAVEGLDDEDNLRLQIDL
                     SPISAFSLWGDVLAEKRSNERDELVIHRLESLEERGFDQPKGTSASRARFSVAGVYPI
                     PLLSRLLGSRSKHFELRDGLLFDVTKTVSDSLMVYDVRDPERIRRVGHYSVPNDQIRA
                     LLPLADGRTLLGGYKLHVVQPPKG"
BASE COUNT         1062 a         1121 c         1040 g         1004 t
ORIGIN      
        1 cccaatatgg tcggcaaacc gttcgatatc cgacaaaccg tgtgatgaga tgagaacgga
       61 atggtgtcca tcctcaacca atttcagtat ctccgcgaag aattcctttt tcgatatcgc
      121 gtccaagccg attgtcggtt catccagaag caacaggggt ggacgacgag ggagaaccag
      181 agtaagagca agtttcgtcc tctcaccaag tgacagctcg gagatcttgg catccctgag
      241 caggtgaaac cgcgccaaca gattctcgca atagtcaaaa tcccattcag gatagaaggc
      301 gcaaatgaaa gagatgagcg caccgacagt tcgccacgca aggaaatcca gatccggact
      361 cacataaccg acacgcttct tcagttcgac ttcgtctcgg atgtgatcga aaccgagaac
      421 cttgattgtt cctgagtcat ttctccccag accgacgacc aagtcaatcg tggttgtctt
      481 ccctgcgccg tttggtccga ttaacccata caccgcaccc actggaacgg tgaggtcgag
      541 aggtcccaga tgaaaagtat agaaagtctt ttccagaccc ctgatctcca acgccgattc
      601 aggactgttc tgactgctca tgaacttccc cctgtgtctt agaattgctc tttttcaagc
      661 cgctcgaaca tctgccgtat ttcgtctctg ctcaatccgc tttccagagc ctctttcagc
      721 gcatcgcgca tgagtgtttg cgcgttctct ctcttgatca tctgatttct gtcctgaccg
      781 tcctctgata caaaagtccc cataccctgc cttcgataga tgatcccctg acgctctaac
      841 tcttcgtagg ctcgtttgac cgtgatcagg ctcaccatga gctgttccgc caactgccga
      901 aaggaaggca acagttcgcc cgatttgagc cgcccttcgg ttatctctgt cttcaatgca
      961 tctaccacct gctggtacag tggttttgcc acagcagagg ttatcggatg cagtagtatt
     1021 ctcttcgttg cgtctttcat tcaggctact gtatatacta tctataaaca gtagtgctgt
     1081 caaggggaag tcggtacggt gttgtcaaga aacatcttac catagcgtca aaatgacact
     1141 atcgggtgac caattatgac acgcagcgtg cattctggct tacaagacgc gctggcaaaa
     1201 agtcggactt tgaagggaga acgtgtatga gttcacactt tagtgtgtct ttatcaagta
     1261 cttatgagaa gacaacctga aggttgaact cttaccatgg actttttgcc agcacgtctt
     1321 acaacgggga gcaagccgtg cagttgggag atcggttatg ggcgaggaca tcttctggaa
     1381 tgacaaccaa aggataagcc gtgggataac cgtcgtctca caattccctc gtacaatgcc
     1441 gtcggagtcc ataacacaag ccagccgaga gagaagttga tcgagttcag tcgggtttcc
     1501 cctgaatagc cttcaacacc agagttctca aattatgcag agtttcacga atggctcttc
     1561 ggtcgatctg tccttcatat ccaacaagat cgtaacgcag ctcgacggcg aatggtgtaa
     1621 gtgagtcaag ccaggtgaat tcctcaggca aagaaatacc cgcttcggta agaagattca
     1681 atagctcggt gagatcatgt gttcgaggaa acgaagcgcc atgagcggac agaagcgctt
     1741 tgagcatttt ctcagcagcc tgctggcaat ggaaaccata gacggcgtca gctacgctta
     1801 cgtcatccat gacaacgtca agcagatgct catcctcggc agccttgtcc agcaaaagcc
     1861 ttgcttgctc aacctgcttg gtagacggtc ttgccatctt gaactgcctc gtaatagacg
     1921 ttgcccggcg tatcccgcca gtagtcgaat cttgccttgt ccacaacaag gatatcaaca
     1981 cccataatca gtccactcaa agcacgtctc agtcgaacag attcctgaac cttgtccgaa
     2041 acgtgttcca acacgaccat aagatccaag tcgctattgt tgttcgcgtt tccgcgggct
     2101 tgagatccga agcagatgat ttccatcgga ttggcaatct ccgcaatgcg ttgcaccgct
     2161 tcccgaatct tctcttctgt cactgcccag atgggctgcg ttttgtcatt catcacaaat
     2221 agctcggctc ccagtgttga acttgtatac catatccatc cgaaatggac aacacattgg
     2281 atgggtcatt gtggtagcaa agcaataatg tcccctctaa cagccgtgca atcatgcccc
     2341 tacgaaacag ggaccatcat tgcagacgag agaggaagga atgaggacgt tggtaccgca
     2401 accaagtcgc attccggttt cctgttgtcg atctgcaata gcaggtttct ctctatcctt
     2461 tcggtggttg aaccacatgc agtttgtagc ctcccagaag cgttcgtcca tccgcaagcg
     2521 gcagaagtgc cctgatctgg tcattgggga cgctgtagtg tcccaccctc cggatgcgtt
     2581 ccgggtcacg aacatcatag accatcaggg aatcggaaac agtcttcgtg acatcaaaca
     2641 acagaccgtc cctgagttca aaatgctttg accttgagcc gagcaggcga gacaacagag
     2701 gtatcggata gaccccggct acggagaatc gagcgcgact tgctgatgtt cctttcggtt
     2761 ggtcaaaccc tctttcctcc agcgattcca agcggtggat gaccagctcg tcgcgttcgt
     2821 ttgatctctt ctctgcgagt acgtctcccc agagcgagaa agcgcttatt ggtgaaaggt
     2881 cgatctgcaa tcgcaggtta tcctcatcat ccagtccttc tacagcaggg agaatccatt
     2941 cgttgccctg acccatggac agcatgtggg tgaaaatctt gtccgctggt tgctcggcaa
     3001 tgaatctcgg aatgcccaac tcccctgaga tgtcgaacag cagcactgac cgtcctcgat
     3061 gcagaatgaa aaggtgattt ccatacgcag acatttctgg aaacagtggc cagctagcat
     3121 cattgtaggt cgttatccct tgatcctcac tgattttgag ataggcggac atgtctatgg
     3181 aagccaaaac cggatctatg tcttcatggc gattgtcggg caacggttcg cgtgccctat
     3241 ccaagtcgag ggcaatcaac tccagtcttt gtcgcttgta gttgaagaac tctcgcccat
     3301 agcgcaaggt ttcctcaggc tcgcaatggt caatgaaggt gtacacgcga tttggctgat
     3361 ttggcgacca ggcgacggga cgcttaatcc actgggagac ggctggcttg gattgatctc
     3421 cctctttcca gaatctgtcc ggcatccaga ttcgcttcga gaaagtcgcc tttccagaga
     3481 cgtcaatctc ggcaagttgg aagtgctcat tgatgattcc cggttgcatc agtgcaaagg
     3541 ttcggtcgcc gaactggtga agcgaactga tgaagttgca gtactcgggc aatgagacaa
     3601 cgtctgattc catgttcgtc ccgagttctg ttgaaacgcc caggcttaac agcaggacta
     3661 ccgccaccag cgaccagcag atcgtccgct gacgcgcgtc gatcgtgatg ttcttcttca
     3721 cggtcacgat ggaaagaaac agtgcagcag cgctgatcag cagcgagcag atcgcaaaga
     3781 gcacgtatcc cttctcaatg tagacaccgt actcgaacag aacgaagaaa tacctgcttc
     3841 gggtggacaa atagtgcttc gccacaggcc agagatggat gggtgggttg accatccact
     3901 cgaacaatga catgaaagag atcggtcgaa gcagcaacgg cgcaaagaca atcagcagac
     3961 ccgcccccag agaaagcaat gccgtggtca ccgtacttcg attcacgcag ccgatgagaa
     4021 atgccacgga gtagaccatg agaacaagcc aaggggttgt ccaggcgata tttgccgtgt
     4081 agctggaaaa ccaaagatac cattctctct cgcgcccgta attggaggtg agtgtctcga
     4141 gaagcaaagg gatggagaag atgatcaaca gcgccacaag cccaccgaag tatttcacgc
     4201 agagaaagcg tccgacggga atgggtt
//
LOCUS       DAOQUC010000156         4227 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_23473_length_4227_cov_3.352589, whole genome shotgun sequence.
ACCESSION   DAOQUC010000156 DAOQUC010000000
VERSION     DAOQUC010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4227
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23473_length_4227_cov_3.352589"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2154)
                     /locus_tag="PKH07_03925"
     CDS             complement(<1..2154)
                     /locus_tag="PKH07_03925"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNT34125.1"
                     /translation="MKLGNLVYRSLNHYWKAHLGVFLGSVVGTAVLVGALGMGDCLSA
                     SLRYFALDRLGSVDLALETGGRFFRTELAAEMSDELNCPVVSCILLRASTSTPDRGAR
                     ANRVQLIGVNDDFPTLWHLERIPLAIRGDEVVVNRSLADQLDIKVGENLIVRVEKPSG
                     FSREAPLTSSDDSQVALRTTVKSVVPDEGMGRFSLHASQIAPFNISLSLSWLQAQLDI
                     ADRANCLLVSNYSDLESAEAALKRTAQLTDYLIELRELPDHDSIELRSPSVFVSDALV
                     QAVQQDYSHALGLFTYLVNEIRLRNQATPYSLITGLGMIGNGAHPSMLSSLVPQDMRD
                     DEIVINQWLADDLGARPGDEVVLEYLTMGAGRLLESHRCALTVRSIIPIEGLAADPEM
                     MPLFPGLQNTDSCRDWDPGFSIDLDRIRPKDEMYWQEHQGTPKGFVTLRKAQQLWGNR
                     FGNVTAIRLPLPTIPIHDLEREILGRLKPSDFGMFFAPVRSQALEAALHAVDFGPLFL
                     GFSFFLIGAALVLMGMIFAFGIEQRAEEVGTLLALGFSIRTVRRLLLLEGFLVTSLGS
                     FVGSLCGVGYARLMLVGLQTVWSDAVGRTDFLFFVSPWTLVVGFSSGVALSLVVVWWV
                     VIKQARAPIRLLLATGQSWQLFELDSKNRTRLSTLAPLVFLSLAALTITIGLGREGVQ
                     SAGVFFAAGSLLLATGISLASSLLGHLASTASLTID"
     gene            complement(2151..2852)
                     /locus_tag="PKH07_03930"
     CDS             complement(2151..2852)
                     /locus_tag="PKH07_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019500227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNT34126.1"
                     /translation="MERLGSALVHLEGVSKSYEAPGVPLAVLNGINLTVAEGESIAIV
                     GPSGSGKSTLLNIVGTLDTPTKGRVCFEGQDLSQFDETQLAYLRNRRMGFVFQLHHLL
                     PQCTALENVLIPTLVTRPKDSRAEQMAYLLLERVGLKDRQNAKPASLSGGERQRVALV
                     RALINEPSLLLADEPTGALDYSSSMGLADLLSELNHNDGVTMIVVSHSLDIARRMNRI
                     LELRDGTLRERDDLK"
     gene            2953..>4227
                     /locus_tag="PKH07_03935"
     CDS             2953..>4227
                     /locus_tag="PKH07_03935"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNT34127.1"
                     /translation="MNYYRAECVFLCGLAILFLAVSPVAASEWPQWRGPFLNGSSDEK
                     GLPERWTKEDGVLWTAPMPGEGGSTPVIQGGCVFLTSTDRKNNDLHAICLDARNGKTR
                     WTKIFSGEGRSVPYNKDAAPSAIADGTRVYFLFGTGDFASYDYQGNEVWRRNLEADHG
                     ALTQLFLYGASPLLFRNRLYLSVLRGNPGGEAHYPKEIVEPHESFLLCVDPDTGRDIW
                     RVRRHFEDTAPENLDSYATPAVWESGSLAEILAIGANYLTAHDWLTGQENWRMRYMEE
                     GKYRQRVVPSPVVVGDRVVCSKPRCGPLFSIRPGRKGEIPFDECEWEYEGLTPDVPSV
                     LSYGGRLYAIHDSKKTLTCLDPESGGKIWEGKLEGEAIYHASPTGADGKIYCMNLAGE
                     IVVVRAADEFHILSRISMGGKPSMSTIAVADGC"
BASE COUNT         1004 a         1082 c         1176 g          965 t
ORIGIN      
        1 gtctatcgtg aggctggcag tggatgccaa gtgtcccaac agagatgagg ccagggatat
       61 tccagtagcg agaagcagag atcccgccgc gaaaaagaca cctgcggact ggacaccttc
      121 acgtccaagt ccgatcgtaa ttgtcagtgc ggccagtgag aggaaaacca gtggagcaag
      181 ggttgacaac cgagtcctgt tcttggaatc gagttcgaag agttgccagg attggccagt
      241 tgctagaagt agtctgattg gagcacgcgc ctgcttgatc acaacccacc ataccaccac
      301 gagagagagt gcgacaccgc tactgaatcc cacaaccaga gtccatggac taacaaagaa
      361 gaggaagtca gttcgtccaa ctgcatcgct ccagaccgtc tgaagtccga cgagcatcag
      421 tcgggcatag ccgacaccac agagcgatcc gacaaaggat ccgagagatg taaccagaaa
      481 gccttcaagg agaagcagtc gtcgcactgt tcgtatactg aaaccgagag ccaacagcgt
      541 ccccacttcc tctgctctct gttcaatccc aaaggcaaag atcattccca taagcacgag
      601 tgccgcccca atcagaaaga aactgaatcc gagaaacaac gggccaaaat caacggcgtg
      661 cagagcagcc tcaagtgcct ggcttcgaac gggagcgaaa aacattccaa agtccgaggg
      721 tttgagtctt ccaagaatct ccctctccaa gtcgtgtatg ggaatggttg ggagaggcaa
      781 tcttatcgct gtaacatttc cgaatctgtt tccccagagt tgctgagcct ttcgaagcgt
      841 gacgaatcct ttcggggttc cctgatgttc ttgccagtac atctcatctt ttggacggat
      901 gcggtcgaga tcaatggaga aacccgggtc ccagtcccgg caactgtctg tgttttggag
      961 ccctgggaat agcggcatca tttccggatc ggcggcgagt ccttcgatgg ggatgatgga
     1021 acgtacggtc aacgcacagc gatggctctc aagcaaacgt cctgcaccca tggtgaggta
     1081 ctctagaacg acctcatctc ccgggcgtgc gccgagatca tcggcaagcc actggttgat
     1141 gacgatctca tcatcgcgca tgtcctgcgg gacaaggctg gaaagcattg agggatgagc
     1201 tccattaccg atcatcccga gacccgtgat cagcgaatag ggcgtagcct gattccgaag
     1261 tcgaatttca ttgaccaggt acgtgaatag tccgagcgcg tgactgtagt cttgctgaac
     1321 cgcctgaacc agggcatctg aaacgaatac gctggggctt ctcaactcaa tagaatcgtg
     1381 gtctgggagt tcacgaagtt caatcagata gtctgtcaac tgtgctgtcc tcttgagcgc
     1441 agcttctgct gactccaagt ctgagtagtt cgacacaaga agacaattgg cacgatccgc
     1501 tatgtccaat tgcgcttgga gccagctcag cgatagtgag atgttgaatg gtgcgatctg
     1561 actggcgtga agagagaatc gtcccatgcc ctcatcgggt acgacagatt tcacggttgt
     1621 ccgtagagcc acttgggagt cgtccgaaga ggtgagcggt gcttcgcggg aaaagccact
     1681 gggcttctcc actcgcacaa tcagattctc tccgaccttg atatcgagtt ggtctgcgag
     1741 acttctgttg actacgactt cgtcgccacg gatagccaga gggattctct ccaagtgcca
     1801 cagggtgggg aagtcgtcat tcacgccgat gagttgaacg cggttggctc gcgcaccacg
     1861 atcgggcgtt gatgtgcttg cacggagcag gatgcacgaa actacaggac agttgagttc
     1921 atcggacatc tctgctgcca actctgttcg gaaaaacctg ccgccggttt ccaaagcaag
     1981 gtctactgat cccagacggt ctaacgcgaa gtacctcaac gatgcagaga ggcaatctcc
     2041 catgcccagg gctccgacaa gaaccgctgt accgacgaca gagccaagga aaacacctaa
     2101 gtgggctttc cagtagtggt tcagactgcg gtagacgaga ttcccaagct tcatttcagg
     2161 tcgtcccgtt cacgaagtgt cccatcgcga agctcgagta tccgattcat cctgcgggca
     2221 atgtctagag agtgagagac cacgatcatc gtcactccat cgttgtggtt cagttctgat
     2281 agcagatcag caaggcccat tgatgaagaa tagtccagag ctcccgtagg ttcatcggca
     2341 agaagcagtg atggttcgtt gatcagagcc ctgaccagcg cgactcgctg tctctcccca
     2401 ccggaaaggg atgcaggctt cgcgttctga cggtctttga gtccgacacg ctccagcaga
     2461 aggtaagcca tttgctccgc tctactgtcc ttcgggcgcg tgacaagcgt tggaatgaga
     2521 acgttctcca aagcggtaca ttgtggaagc agatgatgga gttgaaagac aaagcccatc
     2581 ctcctgtttc gcagatacgc caactgtgtt tcatcaaact gtgagagatc ctgtccttca
     2641 aagcaaaccc ttcccttcgt gggcgtatcc agcgttccga caatgttaag gagagtgctt
     2701 ttccctgaac ccgaaggccc aacgattgcg atggactctc cctctgcgac tgtcagattg
     2761 atgccattca gaactgcaag aggcacacct ggtgcttcat aggacttgct gacgccttcc
     2821 aggtgtacga gtgcggatcc aagtctttcc atgcgaaaat cccttcactg gaaagctttc
     2881 ttccagaaat aggctgtttt gactatagtg atactgcctg ttgaaggcaa gacgtgatga
     2941 gaagggagtg cgatgaacta ctatcgagcc gagtgtgtct ttctgtgtgg actggctatt
     3001 ctctttctgg ccgtttcccc tgtggccgcg tccgaatggc cacaatggcg tggccctttt
     3061 ctgaatggat catccgatga gaagggactg cctgagagat ggacaaagga agacggtgtt
     3121 ctctggactg cgcccatgcc tggcgaaggt ggctcaacgc cggtcatcca gggtggatgt
     3181 gtctttctga cttccactga ccgaaagaac aatgatctac acgcaatctg ccttgacgct
     3241 cggaatggaa aaacgcgctg gaccaagata ttctcgggcg aaggacgatc ggttccttac
     3301 aacaaggatg cagcaccgtc tgcaatcgca gatggaacgc gcgtatattt tctttttgga
     3361 acaggcgatt ttgcatccta tgactatcaa ggcaacgagg tctggaggcg gaatctggag
     3421 gcggaccatg gtgctttgac gcagttgttt ctctatggtg ccagcccgct tttgtttcgc
     3481 aacagactct atctttccgt cttgcgcgga aatcccggcg gtgaagcaca ctatccaaaa
     3541 gagatcgttg agccacatga gagttttctc ctttgtgttg atcctgacac gggcagagac
     3601 atttggaggg ttcggcgtca tttcgaggac acggcaccgg agaatcttga ctcttatgcg
     3661 acgcccgctg tctgggaatc aggttctctg gctgagattc ttgccattgg agccaattac
     3721 ctgacggcgc acgactggtt gacgggtcaa gagaactggc gaatgcgtta catggaggaa
     3781 ggcaagtatc ggcaacgtgt cgttccctcg cccgtggttg tcggcgatcg cgttgtctgc
     3841 agcaagccta ggtgtggccc cttgttttcg attcgtcctg ggaggaaagg cgaaatccct
     3901 tttgatgagt gtgagtggga atatgagggt ctgacaccgg atgtgccgtc tgtattatct
     3961 tacggtggca gactctacgc cattcatgac agcaagaaga cgttgacgtg tcttgatccc
     4021 gagagcggtg ggaagatctg ggagggaaaa ctcgaaggtg aagcgatcta tcatgcttcg
     4081 ccgaccggag ccgatggaaa gatctactgt atgaatctgg cgggcgagat tgttgtggtt
     4141 cgcgctgcgg atgagttcca tatcctctct cggatctcga tgggggggaa gcccagcatg
     4201 tccacaattg ctgtggctga tggatgc
//
LOCUS       DAOQUC010000157         4212 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_23569_length_4212_cov_3.439019, whole genome shotgun sequence.
ACCESSION   DAOQUC010000157 DAOQUC010000000
VERSION     DAOQUC010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4212
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23569_length_4212_cov_3.439019"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            361..873
                     /locus_tag="PKH07_03940"
     CDS             361..873
                     /locus_tag="PKH07_03940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34128.1"
                     /translation="MRIIVVEPEKESRPGISDFLSGQGYLAQPVRHFEDALRRIEQSR
                     GEVDVVFSGCSLELETSSKMFKVMNAFFPSVTLLLKVQDLCSVQACQALDSGVRAFIQ
                     EPVSHGELLSLLKQIEDHRRRRRILRVPRFSEWEPAAPLTPEQKAEMLQTATDRKEES
                     ARQEGLSAQG"
     gene            complement(1359..2543)
                     /locus_tag="PKH07_03945"
     CDS             complement(1359..2543)
                     /locus_tag="PKH07_03945"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNT34129.1"
                     /translation="MSERNFERELSQLVSIGIGLTSTRSLDDLLEQIVHDAREFTRCD
                     AGSLYLVEGDRLRFAVAQNDTLERRGTTAAQGRFLGKYVEISDESMAGYVAGTGTTLN
                     IKDAYDLDPNLAFTINKDFDQANDFRTTSLLLVPMKNREGKTLGVLQLINALDEFGEV
                     TAFESSSENMVQALASQAAVAVENVQLTERLKQAYFQVIIALCAAAEYKDADTALHLK
                     RMSLYSELVAKNLGLSKREQEMILYASPMHDVGKIGIPDAILQKPGALTHQERLEIEN
                     HTIYGADIIARANPGNEEMLKEAETIAISHHEKVDGTGYPKKLRNEKIPISGRIVALA
                     DVFDALTSKRCYKPAFPVEQVLDMIKESRGKHFDPAVVDAFFAGEEQVKAIHSQYQGG
                     AK"
     gene            complement(2669..>4212)
                     /locus_tag="PKH07_03950"
     CDS             complement(2669..>4212)
                     /locus_tag="PKH07_03950"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNT34130.1"
                     /translation="SEQRYRLLAETARDAILVYDLRGRITYANQAAFALTGYSWEELL
                     DRPICDLFPLKLLESEENQERSPRDEDQDHQLEETDLVTCNNLQIPVELSSAQISRDG
                     NVTGVLLVVRDIRERRRAESERGRLEEQLLQTQKMESIGILAGGIAHDFNNLLMAILG
                     NVSLATQDISNVNSVRESLSSVRSAAQRAADLTRQLLAYSGKGKMIVERVCLGETVHR
                     MTQLINVSVSKKAAVIYRISQDDRPVEADASQIRQLVLNLVMNASESLGNQTGTITIT
                     TGAERCSECTLRESYFDDDLREGDYAFIEIQDTGCGMDRETLSRVFDPFFTTKFTGRG
                     LGLAAVSGIVRSHRGVLLVISNPGEGSLFKVLFPVVGSPGDLPSLRDGQAIIQSQGKG
                     TVLMVDDEEQIRTVVGKMIEKMGFTVVFATNGREAVEVFRAKATELAFVLLDLTMPEM
                     SGEEVLAELNKIRHDVKVIATSGVSEQMARSRFEGMNLAGFIQKPYEYSELVSKIHSV
                     VGKDS"
BASE COUNT          982 a         1143 c         1074 g         1013 t
ORIGIN      
        1 ttcgatgggt caagtgtcag gtgaccctcg tgcagaccag gtcgaagcca acaagactct
       61 ggaaatgctc agaagtgccc gatggaaccg gcgcttggcc gccgaacgac tgggcatctc
      121 ctatgaagcg ctccgatggc gcatccgcaa gcaccacctg agtgaggatt tggactgact
      181 tcaaccccaa accttggggt ctcaacccca accgttgtta ctctcgacaa tgcccctatc
      241 agtctatcca aaagatatta tctatctaat atattgtaat acaataactt aaatgaaaag
      301 ctacgcgagt ggcatgagaa ctgctgttta tttcctcacg aaaacatgat gaggcgccga
      361 atgaggatta tcgtagttga gccggaaaaa gagtcaaggc ctggaatatc ggactttttg
      421 tctggacaag gatatttggc acagccggta aggcactttg aggacgctct caggaggatt
      481 gagcagtcca gaggagaagt ggatgttgtg ttctcgggtt gcagtctgga attggagaca
      541 agcagcaaga tgttcaaagt gatgaacgca ttctttccat cagtgacgtt gctgctcaag
      601 gttcaggacc tgtgttctgt gcaagcctgt caggctctgg actcgggagt tcgagccttt
      661 atccaagagc ccgtgtctca cggcgagttg ctctcacttc tgaaacagat tgaggaccat
      721 cgtcgccgca ggagaatcct gagagtgcca cggttctcgg aatgggaacc ggcagcgcct
      781 ctgacacctg agcagaaggc ggaaatgcta cagactgcga cggacaggaa agaggaaagt
      841 gcgaggcagg aaggactttc tgcccaagga tagagaccta ttcagactca gtgggtgtgt
      901 ctgggaatgt tgcggaacgc gcactaacgc ccctgaggaa gggaaggaag gagacggcgg
      961 atgggtagat cggcgataag actctccaaa gaaagatttc actcgctcaa agggagtcaa
     1021 gacgttcgac cagccagatt tgttagttat cgttgctctg ctcagaccgc cgctctcccc
     1081 cgagcaggcc agcgcctgca gtcgtgaggg aaacagactt cttctgagtc tggtttccca
     1141 agagaggtaa tgaatgcaga atatgcacaa atagccctat cgtcccgtcg ggactataga
     1201 tagttcgacc tcccaaacac tgctcttgcc gttttggatg aggggttata cccgacctga
     1261 cgtggtgagg ggttccctgc ccaaaccaaa ccacctcacc cgtcaggttt cctttttgtt
     1321 cttcagtgac catgtgccca accagaatcg agcgccaact attttgcgcc gccttggtat
     1381 tggctgtgaa tcgctttgac ttgttcctct ccagcgaaga aagcgtcaac aaccgcagga
     1441 tcgaagtgtt tgccgcgcga ttccttgatc atgtcaagca cctgctccac aggaaacgct
     1501 ggcttgtagc agcgctttga ggtcaaagca tcaaacacat cagccagcgc gacgatgcgg
     1561 ccggaaattg gaatcttttc attgcgaagt ttcttgggat agccggtacc atcaaccttc
     1621 tcatggtgac tgattgcgat ggtctccgct tccttcagca tctcctcgtt gccgggattg
     1681 gctcgagcga tgatgtccgc gccatagatg gtatggttct caatctcaag tcgctcctga
     1741 tgcgtcagag caccgggttt ctgaaggatg gcgtcaggaa tcccaatctt cccgacatcg
     1801 tgcatggggc tggcatagag gatcatctcc tgttccctct tggacaagcc aagattcttc
     1861 gccacaagct ccgaataaag actcatgcgc ttgagatgaa gcgcggtgtc tgcatccttg
     1921 tattccgccg cggcgcacag agcgatgatc acttggaaat aggcttgttt gagacgttcg
     1981 gtgagctgaa cattctcgac cgcaacggcc gcctgagaag ccagcgcctg caccatgttc
     2041 tctgacgacg actcaaaagc cgtgacttct ccgaactcgt cgagcgcatt gattagttgg
     2101 agaaccccca gggtcttgcc ctcccggttc ttcatcggaa ccaacaacag ggaagtggtt
     2161 cggaagtcgt tggcctggtc gaaatccttg ttgatagtga atgccagatt cggatctaaa
     2221 tcgtaggcat ctttgatgtt gagggtggtt ccggtaccag cgacatagcc tgccatgctc
     2281 tcgtccgaga tctcgacata ttttccaaga aacctgccct gcgcggcagt ggttcctcgg
     2341 cgttcaagcg tatcattctg ggcaacggca aatcgaagtc tgtccccttc cacaagatag
     2401 agacttccgg catcacagcg ggtgaactcg cgggcgtcat gaacgatctg ctccagaaga
     2461 tcatccagcg atcgagtgct tgtcagaccg attccaatac tgaccagttg ggacagttcc
     2521 cgttcgaagt ttctttcgct catggttgtg ttcgtcctgt catcagtcgg tttgctggaa
     2581 acgtcccgac ctgtattgat acatcttgta ttataccccg acaggagtgg ctggcaaatg
     2641 aagttttgcg gtaaggccgt actgaatctc acgaatcctt gccgaccaca gaatggatct
     2701 tgctgaccag ttcggaatac tcgtagggct tctggatgaa accggccaag ttcattccct
     2761 caaagcggct tcgtgccatt tgttctgaga cgccactggt ggcaatcact ttgacgtcgt
     2821 gtctgatctt gttgagttcc gccagaactt cttcaccact catctctggc atcgttaagt
     2881 ccagcaggac gaatgcaagt tctgtggctt tcgcccgaaa gacttcgact gcttcccgac
     2941 cgttggtggc aaagacaacg gtgaatccca tcttctcaat catcttgccg accacagtgc
     3001 ggatctgttc ctcgtcgtcc accattaaca ctgttccctt gccttgagat tggatgatgg
     3061 cctgtccgtc tcgaagactg ggcagatccc ctggagatcc cacaaccgga aacaacacct
     3121 tgaaaagaga tccctcaccc ggattgctga tcaccagaag cacgccccta tggcttcgga
     3181 caattccaga aactgcagcc agccccagtc cacgaccggt gaacttggtg gtaaaaaacg
     3241 gatcgaagac tctcgagagc gtctccctgt ccatgccgca cccagtatcc tgtatctcaa
     3301 tgaaagcgta atccccttcc cgcaagtcgt catcaaaata cgattctctg agagtgcatt
     3361 cgctgcaacg ctccgcccct gtggtgatgg tgatcgttcc agtctggttg ccaagcgatt
     3421 cggacgcgtt catgacaagg ttcagaacca gttgccttat ctgcgaggca tccgcttcaa
     3481 ccggtcggtc gtcttgcgag atgcggtaaa tcacagccgc tttcttggag acagacacgt
     3541 tgatgagctg agtcattctg tgaacagtct cacccagaca gactctctcg acgatcatct
     3601 ttcctttgcc cgaataagcc agaagctgac gggtcaaatc cgctgcgcgt tgcgccgcac
     3661 tgcgtacgct cgacagcgac tctcgaacag aattgacgtt cgaaatgtct tgagtggcca
     3721 gactgacatt gcccaggatg gccatgagga gattgttgaa gtcatgtgcg atgccaccgg
     3781 caagaattcc aatgctttcc atcttctgcg tctgaagcag ctgctcttcc agtctgccac
     3841 gttcgctctc tgcgcgtctt ctctctcgaa tatcccgaac caccagaagc acgccggtca
     3901 cgttcccatc acgcgagatt tgtgcactgc tcagctccac aggaatctga aggttgttgc
     3961 aggtgaccaa gtcggtttcc tcaagctgat gatcctgatc ctcatctctc ggacttcgtt
     4021 cctgattctc ctcactctcc agaagtttca gagggaacag atcacaaatg ggacgatcca
     4081 gaagttcttc ccaagagtaa ccggtcaaag caaaggccgc ttggttggca taagtgatcc
     4141 ttccccgaag atcatagacc agaatcgcat cccgtgccgt ttctgccaac aaccggtaac
     4201 gttgttcact ct
//
LOCUS       DAOQUC010000158         4211 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_23578_length_4211_cov_3.134745, whole genome shotgun sequence.
ACCESSION   DAOQUC010000158 DAOQUC010000000
VERSION     DAOQUC010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4211
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23578_length_4211_cov_3.134745"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1094
                     /locus_tag="PKH07_03955"
     CDS             <1..1094
                     /locus_tag="PKH07_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HNT34131.1"
                     /translation="YVRTEYPLAIERLKTAIEQATEYGFLGDCALKDGFSFTLSILEG
                     AGAFVCGEETSLIASIEGKQPEPRPRPPFPAQSGLWGKPTNINNVETWANIPVIIARG
                     ADWYASIGTEKSKGTKVFSLVGAVRNTGLIEVPMGTSLRDIVYEIGGGIQKDKKLKAV
                     QTGGPSGGCIPADKIDMSVDYERLAESGSIMGSGGMVVMDESNCMVDIAKFFTSFTCE
                     ESCGKCTSCRDGLDAALQILTKITDGEADESDLVLLEELSHAIKDASQCGLGTTAPNP
                     ILSTLQYFRDEYEAHVREKKCPATVCKALFQYRIDTDKCRACGKCKPKCPTQAIEGER
                     KVAHTILIEKCTKCGMCFDVCPFDAVIKV"
     gene            1099..>4211
                     /locus_tag="PKH07_03960"
     CDS             1099..>4211
                     /locus_tag="PKH07_03960"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNT34132.1"
                     /translation="MEDKKLLTVEIDGAQVEVPEGTTILEAATKLGVHIHTLCYNKTL
                     SAYGACRLCLVEVTQGTKTDLRTSCNNVVLPGMRVQTATERVQKTRKIMMELALSRCD
                     HSEKILELAKEIGVEKTRFAKKKDECILCGLCVRMCEERMGKSAIGFAHRGMERKIVP
                     PFDMETDVCQTCGACVSICPTNTMKFETFTVNKPRPIRAEFDVELKSRSPIYIPFPQA
                     MPNWARIDKTKCVHLQTGKCGICKEVCEAKAIDYDQADQEEILNVGAMILAPGIEVFQ
                     ADKKEEFGFGHMPNVVSSLQFERILCASGPFSGHVVRPSDHKEPRRIAFLQCIGSRDS
                     RCGRDYCSSVCCTYATKEAILAREHCPEAELTIFGMDFRTHGKEFEEFMLRAQNESGV
                     RYVRSRVPEVDEDPETHNLFLTYENEVGHTVQEEFDIVVLSVGLDVSKGVKELAERLN
                     VEVDQFGFARTDEHHPLLTSRAGVFVCGAFEAPKDIPETVMQASAAAAAASEILHEAR
                     GTMVVRKEYPPERDVRFESPRIGVFVCSCGSNIAGTVDVKAVTEAAKSFPGVVWAENS
                     IYTCSQDALVTIKERVKEHNLNRVVVASCSPRTHERLFQENIREVGLNRYLFEMANIR
                     DHCSWVHRDNPQKATEKATDLVRGAVAKVRLSEPLPTQYFDINKAALVIGGGVAGMNA
                     ALSLASQGFEVTLVEQSGQLGGLARRVHHTIEGMNVAEYLNDLAQRIEKESNITLHLN
                     TQVSAVSGSVGHFKSTLSGSDGSERQFEHGIIIVATGARELETREYGYGSHPAIMTQM
                     EFDEKMVRGESLDALNDIYMIQCVGSRCDEYPNCSRVCCAEAVKNAIAIKTRKPEANV
                     TVLYRDLRTYGMMEQDYRRAREMGVLFVRFEKDKPPVVSVTETGLSIRFHEPVLNAEV
                     QSPADAIILSVGVRPNEGNKSVAPMLKVPLTKDGYFFEAHVKLRPVDFATDGIFVCGM
                     AHGPKLIKEAAIQGLAAASRAATILSKDKMEALSIVSEVDPRVCSGCRLCNTVCAYDA
                     ISYDETKG"
BASE COUNT          977 a         1045 c         1208 g          981 t
ORIGIN      
        1 tctatgtgcg aactgagtat ccccttgcca tcgagcgact caagactgcc attgagcagg
       61 ctaccgagta tggttttctc ggagactgcg cactcaaaga cgggttctcg ttcactttga
      121 gtatcctgga aggtgcgggt gcctttgtct gcggtgagga gacttcgctc attgcgtcca
      181 ttgaaggcaa gcagcccgag ccaaggcctc gaccgccttt tcccgcgcag tcagggctgt
      241 ggggcaaacc gactaacatc aacaatgtcg agacatgggc aaatatcccc gttatcattg
      301 cccgtggtgc agattggtat gccagtatcg gtacggagaa gagcaaaggc accaaggtct
      361 tctcgcttgt cggtgctgtg cgtaacacgg gtctcattga agtgcccatg gggacttcac
      421 tgcgagatat tgtctatgag atcggtggcg ggattcaaaa ggataagaaa ctgaaggcgg
      481 ttcagacggg gggaccgtcc gggggatgca taccagcaga caagatagat atgtccgtgg
      541 actacgagcg tttggcggag tcgggttcca tcatgggatc gggtggcatg gttgtcatgg
      601 acgaaagcaa ctgcatggtt gacattgcga agttctttac gtccttcacc tgtgaggaat
      661 catgtggcaa atgcacgtcc tgccgcgacg ggttggacgc ggcattgcag attctgacca
      721 agataaccga tggcgaagcg gatgagagtg atctggtgct gctggaagag ctgtcccatg
      781 cgatcaagga cgcctctcaa tgcggtttgg gaaccacagc tcccaatccc attctgagca
      841 cgcttcagta ttttcgcgac gagtatgaag cgcacgtcag ggaaaagaag tgcccagcga
      901 cggtctgcaa ggcattgttt caatatcgaa ttgacacgga caaatgcagg gcttgcggca
      961 agtgcaaacc caagtgcccg actcaggcga tcgaaggaga aagaaaggtt gcgcatacga
     1021 ttctcatcga gaaatgtacc aaatgcggta tgtgtttcga tgtttgtccc tttgatgcag
     1081 tcataaaggt ctagaatcat ggaagacaag aaacttctca ccgtggagat agatggagcg
     1141 caggttgagg ttcccgaagg aacgacgatt cttgaggctg ccaccaaact gggcgttcac
     1201 attcacacac tttgctataa caagacactc tccgcgtatg gagcctgtcg gctctgtctc
     1261 gttgaagtca cacagggaac gaagaccgat cttcgaactt cctgcaacaa cgtggttctt
     1321 ccggggatgc gcgtgcagac ggcaaccgaa cgggttcaaa agacacgcaa gatcatgatg
     1381 gaacttgcgt tgtctcgctg tgatcattcc gagaagattc tcgaactcgc caaagagatt
     1441 ggtgttgaga aaacgcgttt cgccaagaag aaggacgagt gtattctgtg cggtctttgc
     1501 gttcgaatgt gtgaagagcg catgggcaag agcgccatcg gttttgcgca ccgcggtatg
     1561 gaacgcaaga tcgtgccgcc cttcgatatg gaaaccgatg tctgccagac ttgtggtgcg
     1621 tgtgtttcga tttgcccgac taacaccatg aagtttgaga ccttcacggt gaacaagcct
     1681 cgtcccattc gtgcagagtt cgatgttgag ctgaagagtc gctcaccgat ctatattccc
     1741 tttccgcagg cgatgccgaa ctgggcgcgt attgacaaga caaagtgcgt tcatctgcag
     1801 accggaaagt gcggcatctg caaagaagtc tgtgaagcga aggccattga ctacgatcag
     1861 gccgatcagg aagagattct caatgtagga gcgatgatcc tagcgccagg tattgaggtt
     1921 ttccaggccg acaagaaaga ggagtttggc tttgggcaca tgcccaatgt cgtcagctca
     1981 cttcaattcg aacgcattct gtgcgcatca ggtcccttca gcggtcacgt tgttcgaccc
     2041 tcggatcaca aggaaccgag acgcattgcg tttctacagt gcatcggaag ccgcgattcc
     2101 aggtgtggtc gggattactg ctcttcagtc tgctgtacct atgcaaccaa ggaagccatc
     2161 cttgctcgtg agcactgtcc tgaagcggaa ctcactattt tcggcatgga ttttcggacg
     2221 cacggcaagg agttcgagga gttcatgctt cgtgctcaga acgagagtgg agtcaggtat
     2281 gtgcgttcgc gtgttccgga agtggacgaa gatcccgaaa cgcacaatct gtttcttact
     2341 tacgagaacg aagttggcca caccgttcag gaagagtttg atattgtcgt tctctccgtt
     2401 ggtcttgatg tttccaaagg ggttaaggaa cttgcggagc gcctgaatgt cgaagtagat
     2461 cagttcggat ttgccagaac tgacgaacat catccgctgt tgaccagtcg cgcgggagtt
     2521 ttcgtatgtg gcgcttttga ggctcccaaa gacattcctg aaactgtcat gcaggcatcg
     2581 gcagcggcgg cggcggcttc ggagattctt cacgaagcgc gaggaaccat ggtcgtccgc
     2641 aaggaatatc ctcctgaacg cgatgttcgt ttcgagtctc cacgaatcgg cgtcttcgtc
     2701 tgttcctgcg gttccaacat tgccggaacc gtggatgtga aggcagttac tgaggccgcg
     2761 aagtctttcc cgggtgtggt ctgggctgag aactcgatct acacctgctc tcaggatgcc
     2821 cttgtcacca tcaaagagcg tgtgaaggaa cacaatctca atcgggtggt tgtggcctcc
     2881 tgttcccctc ggacgcatga gaggcttttt caggagaata tccgtgaggt cggtctcaac
     2941 cgctaccttt ttgagatggc aaacatccgc gatcactgct cctgggtgca tcgggacaat
     3001 ccccaaaagg cgacagagaa agcgacggat ctcgttcgtg gcgctgttgc caaggttcga
     3061 ctttcggagc ctcttccgac gcagtacttt gacatcaaca aagccgccct tgtcattggt
     3121 ggcggtgtcg caggcatgaa tgccgcgctc tcgctggctt ctcaaggttt tgaggtcact
     3181 ctcgtcgagc agtccggtca gctgggcgga ctggcgcgtc gagttcatca caccatcgaa
     3241 ggcatgaacg ttgcagagta tctcaacgat cttgcacagc gtattgagaa ggaatcgaac
     3301 atcactcttc acctcaacac ccaggtgagc gccgtctctg gcagtgttgg ccacttcaag
     3361 tcaactctgt ccggcagtga tggcagtgaa cgccagttcg agcacggaat aatcatcgtt
     3421 gcgacagggg cacgagagtt ggagactcgt gaatacggct acggcagcca tccggcaatt
     3481 atgacgcaga tggaattcga tgagaaaatg gtgcggggag aaagcctcga tgccctcaat
     3541 gatatctaca tgattcagtg cgtgggctct cgatgtgacg agtatcccaa ctgcagccga
     3601 gtgtgttgtg ccgaagcggt caagaacgcg attgccatca agacccggaa acccgaggcc
     3661 aatgtaactg ttctctatcg cgacctgcgc acctacggca tgatggagca ggattaccgc
     3721 agagcccgtg agatgggcgt gctctttgtt cgcttcgaga aagacaagcc tcctgttgtt
     3781 tcggtgacag aaacagggct atccattcgg ttccacgaac cggttctcaa tgcggaagta
     3841 caatcacctg ccgatgcgat catcctttca gtaggtgtgc gtcccaatga aggcaacaag
     3901 agcgttgccc cgatgctcaa ggttcctctc acaaaggatg gatatttctt tgaagcgcat
     3961 gtgaaactgc gtccggtgga ctttgccacc gatgggatct ttgtctgtgg tatggcgcat
     4021 gggccgaagc tgatcaaaga agctgccatc caaggcctgg cggctgcgtc tcgtgcggcg
     4081 acaatcctct caaaggacaa gatggaagct ctgagtatcg tctctgaggt ggatccgcgt
     4141 gtgtgttcgg gatgtcgtct gtgcaacacc gtctgtgcct atgacgccat ctcgtatgat
     4201 gagaccaagg g
//
LOCUS       DAOQUC010000159         4207 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_23619_length_4207_cov_2.745183, whole genome shotgun sequence.
ACCESSION   DAOQUC010000159 DAOQUC010000000
VERSION     DAOQUC010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4207
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23619_length_4207_cov_2.745183"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            82..1122
                     /locus_tag="PKH07_03965"
     CDS             82..1122
                     /locus_tag="PKH07_03965"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNT34133.1"
                     /translation="MKALVRGFFNSQQSGLVLVIVVLGIVLTVFAGTHEDRRTGTPVN
                     NFLNSYTLMQTATDASFFAIMAVGMTLVIISGGIDLSIGSIYALCGVLMALALKGIGD
                     AGSGLTLGLALLVSLGAGVLCGLLNGVMVVGLRVHPFIVTLGTMWVFRGVAFVASRAQ
                     SILVPNSLTRVAKSSLGLESTLCPVPMLVMLLIAAAGSIFLSKTVSGRNIYAVGGNQQ
                     AARYCGLRVGKILINVYLVSGLTAGIAAFLGSSFYGSASCADATGYELYVIASAVVGG
                     ASLAGGKGSAIGAMLGAVLIVLIRQSIRTLHFDQNYEWIIIGCAIIIAVVLDRLNARM
                     AMRRLIRNAQSG"
     gene            1151..2185
                     /locus_tag="PKH07_03970"
     CDS             1151..2185
                     /locus_tag="PKH07_03970"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNT34134.1"
                     /translation="MKTFLVKWCSVVVLMFGLCSLLLHDIEAQQAVEGRPKKVVIAMI
                     AKSSTNPVFLSARTGAEAAAKDLSEKHKISITVDWRTPPTEDGQVQAQRIAQAVNEGV
                     DAVLISCSDAAKVTGAINDAVARGVPVMTFDSDAPNSRRFAFYGVDDVETGRKVFEEL
                     EKLVKGKTKVAILAGNQNAPNLRKRVEGVREGAKDHPDITILDVFYHVETPQDAAAEV
                     IRVQNAYPEIGAWAMIGGWPLFTQTLLTDLDPEKVKVVAVDALPAQLPYVEKGIAPVL
                     LAQPCYLWGYVSIEKIIDKIVLGAEVPEINRMELVRVTKENLGEWARQLKQWGFTDVP
                     EKYLDLSKEP"
     gene            2212..>4207
                     /locus_tag="PKH07_03975"
     CDS             2212..>4207
                     /locus_tag="PKH07_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011143441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34135.1"
                     /translation="MSTIQPLHAFDWYVIGAFFGVSIAIGLYFSRKGTVSLSDYFVAG
                     RKMTWWIAGTSIVATTFASDTPLVISGWCRTVGIEKNWFWWGGLMGTLLCTFFFARLW
                     RRSGVLTDIEFNELRYSGKPAAGLRMFHAGYGALVKNTIIMGWVTLAMTKILEVTLDI
                     PTLVFLKGHWIPTLIPKGVSPVSVLDWSTVAQFPMIGDAVIPAKVTGILLCFGVAVFY
                     SAVAGLWGVLATDFFQFLFAMLGSITFMIVLLVICGGPTGMVFKAKMTVSEGKVVNKR
                     AEPRDRTDLKTLEKHLHPRHIQELIDAGVFTRDSVEDDTLHWTSSTFREADVQARVAR
                     LTQPVQEAALGLWRSSFTVSKASFADSTEIDKIVHDGLLIETTSPKGAQADYYLLSDI
                     SMSEKSILARLDAAGVRDKSGFMAAWRKDQVVPARKITSFLPPFDLKGGGMMALWSFI
                     VFVGLQWWGGGQGGSFLSQRLFACKNEKHSVLAMLWYNLAHYALRPWPWIVAGIASIW
                     LIPDITFYGTHYDQEYAYVVMFMKHLPTGLKGLMVASLMAAYMSTISTQVNLGASYVI
                     NDLYKRFLHPMASERHNLLVSQMATIVLALIAGIYAFYSSSIASGWFTVLEMLSGAGL
                     VLLVRWYWWRVNAWSEIAAMASSLVLYGLLNWTHLFHRLCT"
BASE COUNT          883 a         1085 c         1209 g         1030 t
ORIGIN      
        1 gcgtgggcta tgcccactcc cttggacaat tgctcggtga atgacacgct tcttctggac
       61 agagtgtaac gaggaaacgg catgaaggct ctagtccgcg gatttttcaa ctcccaacag
      121 tctggacttg tgcttgtcat tgtcgttctt gggatcgtgt tgaccgtttt tgcaggaact
      181 catgaagacc gaagaaccgg cactcccgtc aacaacttcc ttaattccta caccctcatg
      241 cagaccgcaa ccgacgcaag tttctttgcc atcatggcag ttggaatgac gctggtaatc
      301 atctccggtg gaattgacct ttccatcggt tcgatttacg ccctgtgcgg agtcctcatg
      361 gctcttgccc tcaaaggcat cggagatgcc ggttcgggac tgactttggg gctggctttg
      421 ctcgtgagtc tgggtgcggg cgttctctgc ggtctgctca atggagtcat ggtcgtagga
      481 ctgcgcgtgc atcctttcat cgtaacactc ggaacgatgt gggtatttcg cggagttgcc
      541 tttgttgcca gcagggcgca gagtattctc gttcccaatt cgctgacaag ggttgcaaag
      601 agctcccttg gattggagag caccctctgt ccggtaccga tgctggtcat gctcttgatc
      661 gccgcggcgg ggagtatctt tctctccaag acggtctcag gacgcaacat ctatgctgtg
      721 ggtggaaacc agcaggctgc tcggtactgc ggacttcgtg ttggaaagat cctcatcaac
      781 gtctatctgg tcagtggtct cacagcaggt atcgcggcat ttctcggaag cagtttttac
      841 ggttcggcat cctgcgccga cgccacgggc tatgagctgt acgtgattgc atccgcagtg
      901 gtcggtggtg ccagtcttgc cggaggcaag ggcagtgcca tcggcgcaat gctgggcgca
      961 gtgctgattg ttctgatccg tcaatcaatc cgaactctgc actttgatca gaattatgaa
     1021 tggattatca tcggatgcgc cattatcatc gccgttgtac tcgaccgttt gaatgctcga
     1081 atggcgatga ggcggctcat cagaaacgcg cagtctggtt gagaatatgc tgttgcaaca
     1141 ggaggataag atgaaaacgt ttcttgttaa atggtgttcc gtggttgttc tgatgttcgg
     1201 tctgtgcagt ttgctgctgc acgatatcga agcccagcaa gcggttgagg gtcgtcccaa
     1261 aaaggtcgtc attgcgatga tcgccaagag ttcgaccaat ccagtctttc tctcggcacg
     1321 cacgggagcg gaagccgcag caaaagacct gtcagagaag cacaagatct ctatcaccgt
     1381 tgactggcgc actccaccga cggaggatgg acaggtgcag gcacagcgca ttgctcaagc
     1441 ggtcaacgaa ggcgtggatg ccgttctgat ctcatgttcg gatgccgcca aagtcaccgg
     1501 cgcgatcaac gatgcagttg cccgcggcgt gccggtcatg actttcgaca gtgatgcgcc
     1561 gaactccagg cgttttgcgt tctacggagt agatgacgtt gaaacagggc gaaaggtctt
     1621 tgaggaattg gagaaactcg tcaagggcaa gacgaaggtc gccatcctcg ccggcaacca
     1681 gaacgcaccg aatcttcgca agcgcgtgga aggcgttcga gaaggcgcga aggatcatcc
     1741 tgacatcaca attctcgatg ttttctacca tgttgaaaca cctcaggacg cggcggcgga
     1801 agtcattcga gtgcaaaacg catatccgga aatcggcgca tgggcaatga tcggtggttg
     1861 gcctctcttc acacagacct tgttgaccga tctggatccc gagaaggtaa aggttgttgc
     1921 tgttgatgcg cttccggcgc agttgcctta cgtggaaaag ggaatcgctc ccgtgcttct
     1981 ggcgcagccg tgctatcttt ggggatatgt ttccatcgag aagataattg acaagatcgt
     2041 gcttggagcc gaggtgccgg agatcaaccg gatggaactg gtgcgcgtca ccaaagaaaa
     2101 cctcggagaa tgggcacgtc agttgaagca atggggcttc accgatgttc cagagaagta
     2161 tctggatctg tcgaaagaac cttgatcggc ctgcggtgag ggagaagcag attgagtacg
     2221 atacaaccgc tgcacgcatt tgattggtat gtcatcggcg cgttctttgg tgtgtccatt
     2281 gccataggac tgtacttctc ccgcaaagga acggtcagcc tttctgatta ctttgtcgcc
     2341 ggcaggaaaa tgacctggtg gatcgctggc acgtccattg tcgcaacaac ttttgcgtcg
     2401 gacacgccgc tggtgatctc cggctggtgc cgaacagtgg ggatcgaaaa gaattggttc
     2461 tggtggggtg ggctgatggg cacactgctc tgcactttct tctttgcccg cctctggcgt
     2521 cgttcgggcg ttttgacaga catcgaattc aacgaactcc gctattcggg aaagcccgcg
     2581 gcagggctga gaatgtttca cgcaggctat ggtgcgctgg tcaagaacac cattatcatg
     2641 ggctgggtga cccttgcgat gacaaagata ctcgaggtga cactggacat tccgactctc
     2701 gtctttctca aagggcactg gatcccgaca ctcatcccca aaggagtcag ccccgtctcg
     2761 gtgctggact ggtctaccgt tgcccaattt ccgatgatcg gtgatgccgt cattcccgcg
     2821 aaagtgaccg gaattcttct gtgcttcggg gtggccgtat tctacagcgc ggtggcaggt
     2881 ctttggggtg ttcttgcaac agatttcttc cagttcctct ttgccatgtt gggttccatc
     2941 actttcatga tcgtgttgct ggtgatctgt ggcggaccga ccggcatggt cttcaaagcg
     3001 aagatgacag tcagtgaagg caaggtcgtc aacaagagag cggaaccgag agatcgaacg
     3061 gatctcaaga ccctggagaa gcatctccat cccaggcata ttcaggagtt gatagatgcc
     3121 ggcgtgttca caagggattc ggtggaagac gacacgcttc actggacgag cagcacattc
     3181 cgcgaggcgg atgttcaagc ccgagtcgcc agactgactc aaccggttca ggaagccgcg
     3241 ctcggactct ggaggtccag tttcaccgtg agcaaagcca gttttgccga ctcgacagag
     3301 atcgacaaaa tcgttcacga cggactcctc atcgagacaa cttcgccgaa aggggcacag
     3361 gcggactact atcttctcag cgacatctca atgagcgaga aatccatcct cgcacggctc
     3421 gatgccgcag gcgtccggga caagagcggc ttcatggcag cctggagaaa agaccaggtc
     3481 gtacctgctc ggaagatcac cagtttcctg ccgcccttcg atctcaaagg cggcggcatg
     3541 atggcgttgt ggtcgttcat cgtgtttgtc ggactccagt ggtggggtgg cggccagggg
     3601 gggagttttc tctcacaacg gctgttcgcc tgcaagaacg aaaagcattc tgtgctggca
     3661 atgctctggt acaaccttgc acactatgcc ctcaggccat ggccttggat tgtggcaggg
     3721 atcgcctcga tctggcttat tccggacatc acgttctatg ggacacacta cgatcaggaa
     3781 tacgcctacg tggtcatgtt catgaagcac ctgccgacgg gactgaaggg tttgatggtc
     3841 gcttcactta tggccgcgta catgtccacc atctccaccc aggtcaatct cggtgcctcc
     3901 tatgtcatca acgatctcta caagaggttt ctccatccga tggcgagcga acgtcataat
     3961 cttttggtgt cgcagatggc aacgatcgtg cttgctctga ttgcgggcat ttatgccttc
     4021 tattcctcat ccattgcctc gggttggttc actgtgctgg aaatgctctc tggagctggt
     4081 ctggttcttc tggttcgttg gtattggtgg cgggtgaatg cctggtcaga gatcgcggca
     4141 atggcatcgt cgcttgttct ctatgggctt ctgaactgga cgcatttgtt ccatcgcctc
     4201 tgcacat
//
LOCUS       DAOQUC010000160         4202 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_23651_length_4202_cov_3.050157, whole genome shotgun sequence.
ACCESSION   DAOQUC010000160 DAOQUC010000000
VERSION     DAOQUC010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4202
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23651_length_4202_cov_3.050157"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1321
                     /locus_tag="PKH07_03980"
     CDS             <1..1321
                     /locus_tag="PKH07_03980"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HNT34136.1"
                     /translation="TDFAHAEFPRRNAVVEAVENVKGAVVNINTSSIRQGIVRMPSFG
                     PFEDFFPPFYSRQNYVAKSLGSGVIVTADGYVVTNAHVVQEPIEIQVTLVNGDTYDAT
                     YIGSDPRVDVAVLKIEGATQEFFHLPLESHDELMIGETVIAVGNPYGLQNTVTTGVVS
                     AIDRQISTNQGTEFTGIIQTDAAINPGNSGGPLLDINGKLIGLCVAIRAEAENIGFAV
                     PVAKVIRAFNELARGSACLQEILGIEIQNLTEPLSRHFGLPNTDGVVIVELAAGSLGE
                     EIGLQVGDVLVALEQQQIKNAQAFCEFIASMDPSKMDRVRLSVLRNGQRKDFQIKAQQ
                     LDKYSTSTRTTTWRGMGVSSIDAALARRTGVRMSDGVVIESVQKGSPAEESGLRKGDL
                     ILQIGKERVSSLADFQRVVGQMEQYESVEMLVRRGSADYVATVRKKG"
     gene            2016..3941
                     /gene="dnaK"
                     /locus_tag="PKH07_03985"
     CDS             2016..3941
                     /gene="dnaK"
                     /locus_tag="PKH07_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010940711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HNT34137.1"
                     /translation="MAKVIGIDLGTTNSCVAVIEGGEPVVIPNAEGGRTTPSVVAFTE
                     SAERLVGMVAKRQAITNPQRTVYSIKRFMGRRLSEVKEEITKVPYKVKADSSQNAVVD
                     IDGKEFTPPEISARILQKMKQTAEDYLGEKVSQAVITVPAYFNDSQRQATKDAGRIAG
                     LEVLRIVNEPTAAALAYGMDKKRDEKIAVFDFGGGTFDISILELGDGVFEVKATNGDT
                     HLGGDDIDQVLIDWLADEFKKEYGIELRNDHMALQRLKDAAEKAKCELSTTTETSIDV
                     PFISADTSGPKHLKMKMTRAQMERMISGLVERTVGPCKQCLSDAGLKPNEIDEVILVG
                     GSTRIPCVQQKVRELFGKEPHKGVNPDEVVALGAAIQAGVLTKDVKDVLLLDVTPLSL
                     GIETLGDVFTRLIERNTTIPIKKSQVFSTADDNQPEVTIRVLQGERPIASANRELGRF
                     NLVGIPPAPRGVPQIEVTFDIDANGIVHVSAKDLGTGKEQKIRIESSSGLNEKEIEKM
                     VKDAEEHAEEDRKRQEEVDARNKADAMAYSVERSLKEFGDKVDASERTKIEDAVAALK
                     KALEGSDLDEIKRKQEELEKASHELSKILYQQAAQAQQQAQSAQGGAEQDTGAAGSSQ
                     KPNEDIVDAEFEVEDNK"
     gene            3966..>4202
                     /locus_tag="PKH07_03990"
     CDS             3966..>4202
                     /locus_tag="PKH07_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002647158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNT34138.1"
                     /translation="MPTYDYECLKCSHSFEAFQKMTDKPLKKCPECGGKVKRLIGAGA
                     GVIFKGSGFYATDYRSKDYTEKAKKEQSESKSESK"
BASE COUNT         1092 a         1052 c         1241 g          817 t
ORIGIN      
        1 gacggacttc gcacacgcgg agtttccgag gcgcaatgcc gtcgtcgagg cggtcgagaa
       61 cgtaaaaggt gccgtggtca acatcaacac ttcttcgatc agacagggaa tcgtcagaat
      121 gcccagtttt ggccccttcg aggacttttt cccgcctttt tactcaagac agaattatgt
      181 tgccaagtct cttggcagtg gagtcatcgt gaccgctgac ggttatgtgg tcaccaacgc
      241 ccatgtggtg caggaaccca tcgaaatcca ggtcacactg gtcaatggag acacgtacga
      301 cgccacctac atcggctccg acccaagggt ggatgttgcc gtcctgaaaa tcgagggggc
      361 aactcaagag ttcttccacc ttcctcttga aagtcacgat gaactgatga tcggagagac
      421 ggtgatcgcc gtgggaaatc cctacgggct ccaaaacacc gtgacgacag gcgttgtcag
      481 cgccatagat cgccagatca gcacgaatca ggggacagag ttcaccggca ttatccagac
      541 cgacgcggcg atcaacccag gtaacagtgg ggggcctctg ctggatatca acggcaagtt
      601 gattggcttg tgtgtggcca ttcgagctga ggctgagaac attggatttg ctgttcccgt
      661 ggcaaaagtc attcgcgcat tcaacgaact ggctcggggt tcggcctgtc ttcaggaaat
      721 cctgggcatc gagattcaga atctgaccga gcctctgagc agacatttcg gattacccaa
      781 caccgacggc gtcgtgatcg tggaactggc tgctggttct cttggcgagg aaatcggctt
      841 gcaggtgggt gatgttctgg tggctttgga acagcagcag atcaagaatg cccaggcctt
      901 ctgtgagttc atcgcctcaa tggatccgtc caagatggat cgtgttcgtc tttccgtatt
      961 gcgaaacggt caacgcaagg atttccagat caaagcacag caactcgaca agtactccac
     1021 atcaacccga accacaacgt ggcggggcat gggcgtttcc tccattgacg ccgcacttgc
     1081 ccgacgcacc ggagtccgga tgagcgacgg cgttgtgatc gaatcggttc agaagggttc
     1141 tccggcagag gagtccgggc ttcgaaaagg ggatctcatt ctgcagattg gcaaagagcg
     1201 cgtctcctcg ctggcggact ttcaacgtgt tgtgggacag atggagcaat acgaatcggt
     1261 ggagatgctg gtccgaagag gctccgcaga ttacgtcgca acagtcagaa agaagggata
     1321 ggaggccgat ggtacataag aaacattcat ccgacagaaa ggatcacgaa aagcaggaag
     1381 ccactgcgga tattctggaa cagtccccgc ctgagcaagg ctgtgaggga agcaaagccg
     1441 aagaagccgt tgcttgcgcg gacggcaaca ctcaggcaga cggtcaggat ccgaagaggg
     1501 ttgtggactt catgcagatt ctcaaggagc gcgatgagtt tctggagcat cttcagcgta
     1561 tgaaggcgga gttcgacaac taccgcaaga gagtgcagcg cgaacgggaa gatctcgtca
     1621 aaagcgccaa ctcagaactg atcgcagatt tgctggagat tgtggacaat ctacatcggg
     1681 cacttgatgc cgcccaggag acaggtgagc aaagaggttc cctggcccag ggagttgaga
     1741 tgacggctca gatgctcttt ggagtcttga gcgcgcgcgg actgaaaccc atcccgggct
     1801 tggcgagccg ttcgatccga cagtgcacga ggcagtcagc accgaggttc gccctgaaat
     1861 tcagcctggg attgtggttc aagaatggca aaagggttac tggctcggag acaaggtcat
     1921 cagaccggcc aaggtggtgg tttccgccgc cgcgccgagt cagagcaata cagattagaa
     1981 gaaagtaccg agttatctcg gaggagggaa aggcaatggc aaaagttatc ggaatcgatc
     2041 ttggaacaac aaactcctgt gtagcggtga tcgaaggtgg agagcctgtg gtcatcccaa
     2101 acgccgaagg cggtcggaca acaccgtcag tggtcgcgtt cacagagagt gcagagcggc
     2161 tggtgggcat ggttgccaaa cggcaagcca tcaccaatcc tcagcggaca gtctactcga
     2221 tcaagcgctt catggggcga cggctgagtg aagtgaagga ggagatcacc aaggttccct
     2281 acaaagtcaa ggcggactca agccagaatg cggttgtgga tattgatggc aaggaattca
     2341 caccaccgga aatctccgct cgcatccttc agaagatgaa gcagactgcg gaggattatc
     2401 tgggtgagaa agtcagtcag gctgtcatca cagttcccgc ctacttcaac gacagccagc
     2461 gtcaggcgac caaggatgct ggacgcatcg ccggcctgga agttctgcga atcgtcaatg
     2521 agccaaccgc ggcggcgctt gcgtacggca tggacaagaa gcgcgatgag aagatcgccg
     2581 tttttgattt tggtggtgga acctttgaca tctccatcct ggaactggga gatggcgtgt
     2641 ttgaggtcaa ggcaaccaat ggtgacaccc acttgggtgg agatgacatt gaccaggtat
     2701 tgatcgactg gctggccgat gagttcaaga aggaatacgg cattgagttg cgcaatgatc
     2761 acatggcact ccaaaggctc aaggatgctg cggagaaggc caagtgcgaa ctctcaacta
     2821 ccacagagac aagcattgat gtgcccttca tctccgcaga tacttcaggc cccaaacatc
     2881 tgaagatgaa gatgacgcgt gctcagatgg agcgcatgat ctccggtctt gtggagcgta
     2941 cagtcggccc ctgcaagcaa tgcctgtcag atgcgggctt gaaacccaat gagattgacg
     3001 aagtgattct ggtaggcggc tcaacccgaa tcccctgcgt ccagcagaag gttcgcgaat
     3061 tgtttggtaa ggaaccgcac aaaggagtga atcccgatga agttgtggcg ttgggtgcag
     3121 ccatccaggc gggcgttctc accaaggacg tcaaggatgt ccttctcctc gatgtcacgc
     3181 cactgtcact tggcatcgag acattgggtg acgtgttcac gcgactgatc gaacgcaaca
     3241 ccaccattcc tatcaagaaa agccaagtct tctccaccgc agacgacaat cagccggaag
     3301 tcaccattcg ggtgcttcag ggagagagac ctatcgcttc agcgaacagg gaactgggac
     3361 gctttaacct tgtcgggatt ccaccagcac ctcgtggcgt gccgcagatc gaagtgacct
     3421 ttgacattga tgccaacggc atcgtccatg tttcggccaa agatctcggt accggcaaag
     3481 aacagaagat ccgcatcgag tcttccagcg gcctgaatga gaaggaaatc gagaagatgg
     3541 tcaaggacgc tgaggagcac gccgaggaag accgcaagcg tcaggaagaa gtggacgcca
     3601 ggaacaaagc cgatgcgatg gcatattccg tcgagcgctc actgaaggag ttcggcgaca
     3661 aggttgacgc gtcagaacgt accaagattg aagacgcagt tgccgcgctc aagaaagcgc
     3721 tcgaaggttc cgaccttgac gagatcaagc gcaaacagga agaactcgag aaggcttctc
     3781 atgaactcag caagatcctc tatcaacaag cagcccaggc gcaacagcag gcacagtcgg
     3841 ctcagggcgg tgccgagcaa gatactggtg ctgctggttc ttcccagaaa ccaaacgagg
     3901 atattgtgga tgcggaattc gaggttgagg acaacaagta aaccagttct aaggagtaac
     3961 ccgaagtgcc aacgtatgac tacgagtgcc tgaaatgcag ccacagtttt gaggcatttc
     4021 agaagatgac agacaaaccg ctgaagaaat gtcctgagtg tggtggcaag gtaaagaggc
     4081 tcatcggagc cggcgcaggc gttattttca aagggtcggg attctacgcc accgactacc
     4141 gcagcaaaga ctatacggaa aaggccaaga aggagcagtc ggagagtaag tcggaatcca
     4201 ag
//
LOCUS       DAOQUC010000161         4202 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_23654_length_4202_cov_2.921389, whole genome shotgun sequence.
ACCESSION   DAOQUC010000161 DAOQUC010000000
VERSION     DAOQUC010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4202
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23654_length_4202_cov_2.921389"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1443)
                     /locus_tag="PKH07_03995"
     CDS             complement(<1..1443)
                     /locus_tag="PKH07_03995"
                     /inference="COORDINATES: protein motif:HMM:NF022623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HNT34139.1"
                     /translation="MRKVSAICFVVVLLGCARVPDQGLEKLLTSRPMRASSSDPNWQN
                     GNWDARPIAPGETITLGDLEGPGIIKHIWFTIAAADALYARNTVFRIHWDGEEDPSVE
                     APMGDFFAVGHGMLKPLHTFPINISSGGRAYNCYWRMPFRKTARLTMTNDSDKPVHAL
                     YWYIDWAKEQKLPKDSAYFHARYRQEYPCTPGQNYVLLEAEGRGHYVGTVQSVIMAEP
                     GWYGEGDDFFFIDGEQEPSLRGTGTEDYFCDAWGFRPVQGLFYGIPIWEGMDRGDRGT
                     AYRWHIPDPISFTKSLRVEIEHKGSRNDGQGHQYTGFEERPDCFSTVAYWYQIEPHKP
                     FGHVPPVAQRTLPQIILEAEAQIESATFSPDTKSIQDHPACSGGKQIFFTPSVRDASF
                     EVTFDVAETLTGVVELLLLHSWDYGIYNVSLNGSLILKEANLYAPSSEIESHKTPLAE
                     LSSGKQTLLFECVGSDPRSKLGHNPGYYCGF"
     gene            complement(1680..1859)
                     /locus_tag="PKH07_04000"
     CDS             complement(1680..1859)
                     /locus_tag="PKH07_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013459706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34140.1"
                     /translation="MAEEKKKCVGDHLGHLCVLAGSAKFDKIKELAKEPKFICFNCGR
                     VAHSDKNLCNPMPLE"
     gene            2520..>4202
                     /locus_tag="PKH07_04005"
     CDS             2520..>4202
                     /locus_tag="PKH07_04005"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HNT34141.1"
                     /translation="MIQLTDKEKAEIIERIQNDKPLPAKYRATLFEDAKGVELIWKGK
                     RNEVTNVVLPFQTIEQIDEPRDESHRPEYVDDLWSDPKTGRQVGGWTNKLIWGDNKLV
                     LSSLKNGPMRREIENAGGLKLIYIDPPFDTGADFSFNVEVGDGESVTKQPSVLEEIAY
                     RDTWGENGERYLQYIYERLILMRDLLHPAGSIYVHCDWRLNCHIRILLEEILGKFNLI
                     NQISWKRYAAHNLSQKRYDAITDTIIAFAVDWPENIFTPQFGITQECEMESRFPHIEA
                     ETGRRYQHVALEQASNASSKGEARYIAGRRVVSEVGWRWSQETFDERLRENPNLIHWT
                     KAGRPRYKLYVDEYEGSPLGDIWTDIGYLSANDSQGVGYPTQKPEALLERIIKASSNE
                     GDLVADFFCGSGTTLAVAEKLGRKWIGCDLGRFAIHTSRKRLIGVQRRLKAEGKPYRS
                     FEILNLGKYERQYFVGINLSLPETEREKESRKKETEYVRLVLEAYKAQPVSQSPPFHG
                     RKGKVMIVVGPIDAPVTQALVDEVVNGCLAQGLKMVDVLGFDFEMGLVPQAQN"
BASE COUNT         1062 a         1087 c         1122 g          931 t
ORIGIN      
        1 aaagccgcag tagtaacctg ggttgtgtcc cagctttgag cgcggatccg aaccgacaca
       61 ttcaaaaagc agtgtctgct tgccagagga gagttctgcc agcggtgtct tgtgcgattc
      121 gatctccgag gatggtgcgt acaggttcgc ttccttcagg atcagacttc cattgagtga
      181 aacgttgtag atgccgtaat cccatgagtg aagcagaagc agttccacaa caccggtcag
      241 cgtctcagca acatcaaagg tgacttcaaa actggcgtcc cgaacgctgg gagtgaagaa
      301 gatctgtttt ccaccgctgc acgcaggatg atcctgaata gacttggtat cgggtgaaaa
      361 cgtcgccgac tcaatctgcg cctctgcttc aagaatgatc tggggtagtg tccgctgggc
      421 aaccggagga acgtgtccaa acggcttgtg cggttcgatt tgataccagt aggcaaccgt
      481 tgaaaagcag tcagggcgct cctcaaatcc cgtgtactgg tgaccttggc cgtcattgcg
      541 cgagcctttg tgttcaatct ctactcgaag ggatttggtg aaagagatcg gatcgggaat
      601 gtgccagcga taggcggtgc cacgatcccc tcgatccatg ccctcccaga tagggattcc
      661 gtagaacagc ccttgaaccg gtcgaaagcc ccaggcgtca cagaagtaat cctctgttcc
      721 cgtgccgcga agtgagggct cctgttctcc gtcaatgaag aaaaagtcgt caccctcacc
      781 gtaccatcca ggttccgcca tgatcacaga ctggacggtt ccgacatagt gaccacgccc
      841 ctcggcttcg agaagcacat agttctggcc tggtgtgcaa ggatactcct gtcggtatcg
      901 tgcgtggaaa tacgccgagt ccttgggcag cttctgttct ttcgcccagt caatgtacca
      961 atacaaggca tgaaccggtt tgtcagagtc gttagtcatc gtcaaacggg cggtctttcg
     1021 aaacggcatc ctccagtaac agttgtaagc ccgaccgccg gaagagatgt tgatcggaaa
     1081 ggtatgcagt ggtttgagca tcccatgtcc tacggcgaag aagtccccca tcggtgcttc
     1141 aacgctcgga tcctcttcac catcccagtg gatgcgaaaa acggtattcc gagcatacag
     1201 ggcatccgct gctgcaatgg tgaaccagat gtgtttgatg atgccaggcc cctcaagatc
     1261 gccaagggtg atcgtctcac ccggcgcgat ggggcgagca tcccagttgc cgttctgcca
     1321 attcggatcg gaactgctgg cacgcatcgg gcgactggtc agcaacttct ccagaccctg
     1381 atccggcact ctcgcacaac cgagcaaaac aacgacgaaa cagatcgcag agactttcct
     1441 catgacccaa atcctccaaa aaaacgtttg cttctaagaa ccagattctt ttgccacccc
     1501 ttgtcggacg aggcgaaaaa agccttcaca ccagaatctt ccgagcaggt tagcaccagc
     1561 aggcgtgtgg gctatgccca ctccttcgga cgcttgctca atcaatgata cgcaccctct
     1621 ctcctccgtg tcgccagaag caggccagtt ctgacgcggg atacagaggt tagtagatac
     1681 tattccaagg gcatcgggtt gcagaggttc ttgtccgaat gcgccactcg accgcagttg
     1741 aagcagatga atttcggttc tttggccaac tctttgattt tgtcaaactt ggcactgcct
     1801 gccaagacgc agagatgtcc gagatgatcg ccaacacact ttttcttctc ttccgccatg
     1861 atagcctcct gaatcgcgtg aatcagtccg ctctgttttt ggggaagtat acaaacaatg
     1921 cagtcaaagt ctagagaaga aagcactcag atgcactggc tcaattacac aaaggcggag
     1981 actgtcattc cgaacaactg aggcttcgcg aaggagtgag gaatctctcc ctggattctg
     2041 cagtgtgtag ggtgtagggt ggaacgagtc tgcgagttcc accctctttg ctacattctg
     2101 aaaatccgtg aaatcagcat aatccgttta atccgtggtt cagacaatca atccgccgcc
     2161 tcccccaaag gatgcctgcc tcttcaagac gaaagatttt ctactcccac aatcggtgca
     2221 tcatgtatat tatcacttca aagatcacgc cttacggcgt aacgacgaac cggacaacag
     2281 gaggtttctt tagcgaacaa ataaacacaa ttagaaaata agcgatttca agaacgactg
     2341 agttcggaaa ctagcacatt ccctacgtga agaaagtcgt tgcttgggtt cgctccactt
     2401 tggagcggac tcgaagcatg ttcttcttca caggctgtgc taggcctccg aactagagca
     2461 ctgcaacggg catccgctcc ttctcttttg tgggggctgg ggcgagcgag ggagacacaa
     2521 tgatccaact gaccgacaaa gagaaagcgg agattattga gcgcatacag aacgacaagc
     2581 ccctgccggc aaaataccgc gcgacactgt tcgaagacgc caaaggggtc gagctgatct
     2641 ggaagggaaa acgaaacgaa gtcacgaacg tagtgcttcc gttccagacc atcgagcaga
     2701 tagatgaacc gcgagacgaa tcccaccgac cggagtatgt tgacgaccta tggtctgacc
     2761 ccaaaacggg aagacaggtg ggcggctgga cgaacaaact catctgggga gacaacaaac
     2821 tcgttctttc ctcactcaag aacggtccca tgcgccgcga aattgaaaac gccggcggac
     2881 tgaaactcat ctacattgac ccgccgttcg acaccggcgc ggacttctcg ttcaacgttg
     2941 aagtgggtga tggcgagagc gtaaccaagc agcccagtgt ccttgaggag attgcctatc
     3001 gggatacatg gggagaaaac ggcgagagat atctccagta tatctacgaa cggctcatat
     3061 tgatgcgtga tcttcttcat ccggcaggaa gcatctacgt gcattgtgat tggagactca
     3121 attgccacat tcgaattctt ctcgaggaaa tccttgggaa gttcaatctg attaaccaaa
     3181 tatcgtggaa aagatacgcg gcccataatc tttcacagaa acgttatgat gcaatcactg
     3241 acactatcat cgcttttgca gttgactggc cggagaacat attcacaccc cagtttggta
     3301 tcacccagga atgcgagatg gagtctagat ttccccatat tgaggcagag actggacgta
     3361 gataccaaca cgtggcgctg gaacaagcgt caaacgcgag cagcaaagga gaggcgcgct
     3421 acatcgctgg tcgtagagta gtatcagaag tcggatggcg ctggtctcag gagacttttg
     3481 acgaaaggct gcgggagaat cccaacctaa ttcactggac caaagcaggg cgtcctcgct
     3541 acaaactcta tgtagacgag tatgagggta gcccgcttgg tgacatctgg acagatattg
     3601 gttatctgtc agccaatgac tcgcaggggg tcggttaccc cacgcagaag cccgaagccc
     3661 ttctcgaacg aatcatcaaa gcttcttcca acgaaggtga tctggttgct gacttcttct
     3721 gtggttccgg aacgacgttg gcggttgcag agaagttggg tcggaagtgg atcggatgtg
     3781 atctgggacg gtttgcgatt cacaccagcc ggaagcggct cattggcgtt cagcgccggt
     3841 tgaaagccga aggcaagccg taccgcagtt ttgaaatcct gaacctgggt aagtacgagc
     3901 gacagtactt cgtcggaatc aacttgagcc tgcccgaaac cgaaagggag aaggaatcgc
     3961 gaaagaagga aacggagtat gtgcgtcttg tgctggaagc gtacaaggcg cagcctgtct
     4021 cgcagtcgcc accgttccat ggtcgcaaag ggaaggttat gattgtcgtg ggtcccatag
     4081 atgctccggt gacacaggcg ctggtagacg aagttgtgaa cggctgtctt gctcaggggc
     4141 tgaaaatggt tgatgttctc ggcttcgact tcgagatggg cttggttcct caggcgcaga
     4201 at
//
LOCUS       DAOQUC010000162         4195 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_23699_length_4195_cov_3.314976, whole genome shotgun sequence.
ACCESSION   DAOQUC010000162 DAOQUC010000000
VERSION     DAOQUC010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4195
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23699_length_4195_cov_3.314976"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1396)
                     /locus_tag="PKH07_04010"
     CDS             complement(<1..1396)
                     /locus_tag="PKH07_04010"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNT34142.1"
                     /translation="MKKNPIKVVLVSQPNQQAEAVAKRLSGSRSPRFEVEMKAPITDV
                     TEQVSGESNITLLPQQESVQGTRDDRERLISTLAAVAGITTKASTPTQLCEDLAKTLR
                     ATFACDAFFVLRYDSESGELVDVCKGDTIGGTFQIVPNASDSSPLTGILATAVEQRRT
                     LRLLRDSPQSPEKGFVAFGDTERLSLSLLVCPMIAEDRLLGLLSVQSYTPNAYTESDE
                     DLLFAVGQQVGPALVAALLNQEIRKSHASQSLFSKQLSTLLEVTNQLSTAGSFDVLCR
                     RAVELGRERLKFDRLGLWFFDQERRSVRGSFGIDETGCIRDERDQETAILSGTSMSRM
                     LSGQIRFLMEEDSDLRDHTGAVVGHGDYSVACISDGRKVIGCICHDNLLGRTPISDHD
                     NELLQLYASALGALCSLKKSEEQLRESHAELEQRVQERTERLQKTNEDLQFEIDERKR
                     TEQMLRDSERRYRTL"
     gene            complement(1535..2701)
                     /locus_tag="PKH07_04015"
     CDS             complement(1535..2701)
                     /locus_tag="PKH07_04015"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNT34143.1"
                     /translation="MRVAVLVLMVCLSGTTVPSASAQDHPIGDLNEDGYVDETDLMIL
                     MGNWHQGIQPTATPTAFVSETPTDTPSPLQTETQTPTNTNTETPTPTVSQTPTDTNTP
                     TPTVTLTPTPTATQTPTTTVTRTPTRINSPTPTATLRGFWMGNSGVGDDATFGQDSSE
                     EFPRHWVSLGTTSIGQYEVSIAEYALFLNSGGKDSNYHDLMADTVTCGLLRTGVPGAY
                     LYIPASGQSNYPIVYVTWYDAKAYCEWQGKRLPTEAEWERASRWVDAGQQARVYPWGF
                     SWADNYANTFGSTDGYNYACPVNAFSSGASPAGAYNMCGNVWEWCSDWYDPTYYSQTP
                     TGGWSNPQGPLTGTTKVIRGGSYNNWHQSLLRCATRFYKDPAGRYSDVGFRVAQ"
     gene            complement(2755..>4195)
                     /locus_tag="PKH07_04020"
     CDS             complement(2755..>4195)
                     /locus_tag="PKH07_04020"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HNT34144.1"
                     /translation="TVEVKEKLGSGTHSALVTFQNIHKNHAFPKAVSFVVALPAVRME
                     MTIEPDRIPASENAWATVRVKAYDEDGKPVADGTVIDFSITEGELLRAKVKTVNGEAL
                     NYYRCPWGASAQVIAQSGKAEVCGNVWSGKMDQGGAFGVLLDALTGAPVEQGQVGWLE
                     GTRHSVTRDGRFAVSGLQAGQPLMASVPGRFGVVIANIEDSSAVVWKQASLVPAAGGV
                     LAGKSFVLDPRYGGEETGSVAGDHRASECNRLVAIALMQLLQNAGAQVRVTRSDDASL
                     SVDQRLQIANQWPSARYIRIEHEPMTGSRPSLATLRYPGSTIISDFASPIIAEASQYL
                     GLDAGSIRDSEDKEIKDTKGPAVAFIFHTLDHPSMKVDSDGRFLCLREAYVLYRGIAG
                     YHEAPTEGVYALEIRVVDEKTGQPQGNVLVGLGGVESLLSDSVAGMVRFEGLPKGETF
                     VTASKEGYRTSVRNVHTDKGLVEVRLEKD"
BASE COUNT          929 a         1196 c         1066 g         1004 t
ORIGIN      
        1 ggagtgttcg atagcgccgc tctgaatcac gaagcatttg ctcggtacgt ttgcgctcgt
       61 ctatttcgaa ctgaagatct tcgttggttt tctgaagtct ctctgttctc tcctgaacac
      121 gttgttccag ttcggcatgg gactcccgaa gctgttcctc actcttcttc agtgaacaca
      181 gggcgcccag tgccgacgca tacaactgga gcagttcatt gtcatgatcc gagatcggag
      241 tacgacccaa aagattatca tggcagatgc agccgataac ttttcgacca tccgagatgc
      301 aggccacaga gtagtccccg tgtccgacaa cggcccctgt gtgatcacga aggtctgaat
      361 cctcttccat cagaaaacga atctggccgg agagcatcct gctcatagat gttccggaaa
      421 gaattgcagt ctcctgatcg cgctcgtcac gaatacatcc cgtttcatct ataccgaaag
      481 atcctcgtac gcttctgcgc tcctggtcga aaaaccagag ccccagtcga tcgaacttta
      541 gcctttctcg acccaattcg acggcacgcc gacacaagac atcaaacgaa ccggcagtac
      601 tcaattggtt ggtcacttcg agtagcgtgg acaactgttt tgagaacaga ctttgactgg
      661 catggctctt gcgaatttcc tgatttagca acgccgcaac caacgcaggg cccacctgct
      721 gtccgacagc aaacagcaga tcctcatcgc tctcggtgta agcgtttgga gtgtaacttt
      781 gcacagaaag caatcccagc aaacggtctt cagcaatcat cgggcaaacc aaaagggaca
      841 acgacaatcg ttcggtgtcg ccaaaggcca caaatccttt ttcaggactc tgaggtgaat
      901 cccgaagcag cctgagcgtt ctcctttgct ccactgcggt cgccagaatt ccggtcaacg
      961 gagaggaatc ggaagcatta ggaacgatct ggaatgtgcc acctatggtg tcgcccttac
     1021 agacatcaac aagttcccct gattccgaat cgtatcgaag aacaaaaaag gcgtcacagg
     1081 caaaggtcgc tcgaagcgtc ttagccaaat cctcacacag ctgcgtggga gtggatgcct
     1141 tggttgtgat gcccgcaacg gcagccaaag tgctgatcag tctttctcga tcatccctcg
     1201 ttccttgaac agattcttgc tgtggtagga gagtgatgtt ggattctccg gagacctgct
     1261 ctgtaacatc tgtgattggc gctttcatct ccacttcaaa acggggactg cgcgatccag
     1321 agagccgctt ggcaacagcc tctgcctgtt ggtttggttg ggagaccaaa accaccttga
     1381 tcgggttctt cttcattacc ttattctgga aagccttgtg tcaaggcagt cacctgattt
     1441 gaatcttccg tccccaacac gctcacacct gatacgctcc tcgagaacgt ggtttgtccc
     1501 gtcatctcag tctttcttgt tgagtgcagt gttctcactg cgcgactcga aacccaacat
     1561 cagagtaccg gccggccgga tccttgtaga atcgggtcgc acagcgcaac agggactggt
     1621 gccagttgtt gtaactgccg ccgcgaatga ctttggtggt gcctgtcaga ggcccttgtg
     1681 ggttgctcca accgcccgtg ggagtctggc tgtaataagt gggatcgtac cagtcactac
     1741 accattccca gacgtttcca cacatattat acgctcccgc aggacttgct ccactggaaa
     1801 aagcattcac aggacaggca tagttgtaac cgtccgttga cccaaatgtg ttggcgtagt
     1861 tgtcagccca cgaaaacccc caaggataga ctcgtgcttg ttgaccggca tccacccagc
     1921 gggatgctct ctcccattcc gcttcggttg gcaggcgttt tccctgccat tcgcaatatg
     1981 ccttcgcatc gtaccaggtg acgtaaacga tgggatagtt gctctgccct gaggcgggaa
     2041 tatacagata agctcctggg actccggtgc gaagcagacc acaggtgacc gtatcagcca
     2101 tcaaatcatg gtagttgctg tctttgccac ctgagttcag gaaaagggca tattcagcaa
     2161 tgctcacttc gtattggcca atagaagtcg tcccaagact gacccaatgc ctgggaaact
     2221 cttcgctgct gtcctgacca aaggtggcat cgtcccccac acccgaattg cccatccaga
     2281 atcctctcaa ggttgctgta ggtgttggcg agttgattcg ggtcggtgtt ctagtgacgg
     2341 tggttgttgg tgtctgagtc gctgtcgggg tgggagtcaa cgtgactgtc ggtgttggtg
     2401 tgtttgtgtc ggttggtgtt tgagacaccg tgggagtagg tgtctctgta ttcgtgttgg
     2461 tcggagtctg agtttccgtt tgcagaggcg agggcgtatc ggttggggtt tctgaaacga
     2521 aggctgttgg ggttgccgta ggctggatgc cttggtgcca attgcccatg aggatcatca
     2581 ggtctgtttc atccacatat ccatcttcat tcagatcgcc gatcggatga tcctgcgccg
     2641 aagcgctagg aacggtggtt ccgctgaggc aaaccatgag cactaggact gcaacgcgca
     2701 cgatgctcct ccttctcggc ttcgcagaga ctcggcttgg ccgaaatcca cctgctaatc
     2761 cttctccagt cgcacttcca ccagaccctt atctgtatgc acgtttctca cactggttct
     2821 atagccttct tttgaggcag tgacaaaagt ttctcccttg ggaagtccct caaaacgcac
     2881 catgcccgca accgaatcac tcaggagcga ctcgacccct ccaagtccga ccaggacatt
     2941 gccttgtggc tgacctgtct tttcatccac aacccgaatc tccaaagcgt atacaccttc
     3001 tgtcggcgct tcatggtagc cagcaatgcc tcggtacagc acataagcct ctcgaagaca
     3061 cagaaaccgt ccgtcagaat ccaccttcat tgacggatga tccagcgtat ggaagatgaa
     3121 agcaacagca ggccccttgg tatccttgat ttccttgtct tcactgtccc gaatagaacc
     3181 tgcatccagt ccgagatatt gggacgcctc ggcgatgatg ggcgaggcaa aatccgagat
     3241 aatcgtgctt cccggatacc gcaacgtcgc aagggaaggc ctggaaccgg tcatgggttc
     3301 atgttcgatg cggatgtatc gggccgaagg ccactgattg gcaatctgga gacgttgatc
     3361 cacagacaga cttgcatcat cggatcgagt cactcgcacc tgtgcgcctg cattctggag
     3421 caactgcatc agggcgattg ccaccaggcg attgcattct gaagcacggt ggtctcccgc
     3481 tacagaacct gtttcctctc ctccatagcg gggatccaac acaaaagact ttccggcaag
     3541 gacaccaccc gcagcaggaa ccagtgaggc ctgtttccaa acaacagcag acgaatcctc
     3601 gatgttggca atcaccacgc caaagcggcc tggaacgctt gccatcagtg gctggccagc
     3661 ctgaagtccc gacaccgcga accgaccgtc gcgggtgaca gaatgccggg ttccttccag
     3721 ccacccgacc tgtccttgct ccacgggagc gcccgtcaga gcatccagga gcactccgaa
     3781 cgcaccgcct tgatccatct tacccgacca gacatttcca caaacttctg cctttccgct
     3841 ctgtgcgata acctgagccg atgcacccca aggacagcgg tagtagttga gcgcttcgcc
     3901 attcaccgtc ttcacctttg cgcgcaggag ttcgccttcg gtaatactga aatcaatgac
     3961 cgtaccatct gcgacgggtt tcccgtcctc gtcgtaggct tttacgcgaa cggttgccca
     4021 ggcattctca ctggctggaa ttcggtcggg ttcgatggtc atctccatgc gtaccgccgg
     4081 cagcgcaaca acaaatgaga ctgctttggg gaaggcgtga ttcttgtgaa tgttctgaaa
     4141 agtcaccagc gcgctgtggg tgccgctccc gagtttctct ttcacttcca ctgtg
//
LOCUS       DAOQUC010000163         4181 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_23809_length_4181_cov_3.973825, whole genome shotgun sequence.
ACCESSION   DAOQUC010000163 DAOQUC010000000
VERSION     DAOQUC010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4181
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23809_length_4181_cov_3.973825"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..987
                     /locus_tag="PKH07_04025"
     CDS             <1..987
                     /locus_tag="PKH07_04025"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HNT34145.1"
                     /translation="SFLVWAIRGQKVAVPRMVGMEASRAVEEILKNGLTLTGIDDEYS
                     EIIQPGCIIDQNPLPGTVVKRGRGIHLTKSKGMEELEIPSVVGQLAEDAENTLMRSGV
                     RRGQRSDAYHDRVGKGRVVVQEPGAGVRGAKGDAVNILVSLGPRQSGYIMPNVLGMNR
                     ISAEERLRLCGFSRIDVREKTVLDEVAGVVLEQDPGQGALVYLSSPVLMTVSKASEQP
                     QTPAPVIPDTPARVEPDLIHESQAMEEPTEPPPTQIPTLSVVKVRFLYPLPTPKGVGW
                     VEVKMETRSGLQTVYTGVHSKGVLIEFEIFTQPGAVFTVYQDGKQIGTRKVE"
     gene            1036..1698
                     /gene="rpe"
                     /locus_tag="PKH07_04030"
     CDS             1036..1698
                     /gene="rpe"
                     /locus_tag="PKH07_04030"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF004076.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HNT34146.1"
                     /translation="MCKISPSILNSDWGDFRGSIQMLEQQGCDLIHLDVMDGHFVPNL
                     TLGPDLLKHLSKGTTARFEVHLMVSEPEKWVEPYFTDRTETILIHPEGVPHVHRILER
                     IRELGAKPGIVLNPGTPLNALDWVGDCIEQVLLMTVNPGFGGQKFIRGMLPKIAQTRD
                     WLVRKGIDATCPIEVDGGMNLETVPEALNAGARIFVVGAAIFMADDPSKAYRDFVRLV
                     TK"
     gene            1711..2268
                     /locus_tag="PKH07_04035"
     CDS             1711..2268
                     /locus_tag="PKH07_04035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34147.1"
                     /translation="MTLVGSDPTPAGDIVESRSGGKNFPCYSETGVWADSTTGCIAPG
                     CTDGLGQRYGSTYRSVAGAKSAFFRPSLTQTGIYKVYAAWGAGNLRQNGITYEVAHSG
                     GIAKHIIDQTINANTWVLLGQYSFVKGTDGFVEVSNKDIDVSGNMYSGAVRFELVQAQ
                     PDPTSTPTPRPTIGPRTDGTWMMSR"
     gene            2629..3213
                     /locus_tag="PKH07_04040"
     CDS             2629..3213
                     /locus_tag="PKH07_04040"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNT34148.1"
                     /translation="MKADNEHELLQQWKEGSQEAFGELFSRNASDLYNFAYRLTGQTE
                     DARDILQTAALKAFSRGCPSGEATRFYAWMRRVVLNLFLDSERTNRRAGRPSLVQNVD
                     WEDIEFAAVPQSQLASPRAEMDEHQRMQAIRKALDGLPTEYRAVVVLRDVEGLSYAEI
                     ALHLSVGVETVRTRLRRARERLRDTLQPLVSEES"
     gene            3241..4023
                     /locus_tag="PKH07_04045"
     CDS             3241..4023
                     /locus_tag="PKH07_04045"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HNT34149.1"
                     /translation="MKEKETSCRFRDLLSDALDGELSAVQQAQLEEHLLVCEDCRSAL
                     SAYQKVTSLLREMPEASMPTDMVQAVLKETTNTQAVPVTMQENDGVAALAPGRQGSSM
                     RWVRWSAAVAAVLVVAFLWNGLISINNVLSRPMLTSIVASADEELSGIDLVFSFNPRR
                     ISVKTIESALESTGFLIVSHVGKDEVRISMASPERFHPDGGASIVTMQILPKSLSRDT
                     SSFLRLEAVRAYRPDGRPASVTLDQVSVPLPEQCNGDGGTTA"
BASE COUNT          960 a         1024 c         1234 g          963 t
ORIGIN      
        1 tccttccttg tctgggcaat acgtggtcag aaggtggcgg ttccgcggat ggttggaatg
       61 gaagccagtc gagcagttga ggagatattg aagaacggcc tgacattgac cggtattgat
      121 gatgagtaca gtgaaatcat ccaacccggg tgcatcatag atcagaatcc ccttccggga
      181 accgtggtga agcgcggacg agggattcac ctgaccaaga gcaagggcat ggaggagctg
      241 gagatcccca gtgtggtggg acaactcgca gaagacgctg agaacacgct gatgcgttcg
      301 ggagtgcggc ggggacagcg gtcggacgcc tatcacgatc gggtcggcaa ggggcgagtc
      361 gtggttcagg agccaggtgc tggagttcga ggagccaaag gggatgctgt aaacatactg
      421 gtgagtctag gcccgaggca gtccgggtat attatgccca acgttctggg gatgaatcga
      481 atctcagcgg aagagcgcct tcgattgtgt ggattcagtc gaattgatgt tcgagagaag
      541 acagttctgg atgaggtcgc tggagtggtt cttgagcagg atcccgggca gggagctctg
      601 gtatatctta gttctccagt gcttatgaca gtatccaaag ccagcgaaca accccagaca
      661 cctgcgccag tgatccccga cacaccggct cgagtggagc cggatctgat tcacgaatcg
      721 caggcgatgg aagaaccgac ggaaccccct ccgacacaga ttccgacgct gtcggttgtg
      781 aaagtgagat ttctctatcc gttgccaact ccgaaaggtg tgggttgggt cgaagtaaaa
      841 atggagacga gaagcggact tcagacggtc tacaccggcg ttcactcaaa aggtgtactc
      901 attgagttcg agatattcac acagccgggg gccgtattca cggtgtacca ggacggcaag
      961 caaatcggta cccgaaaagt tgaatgagcg gttcttccgc gctcttgaaa ggtcagcaac
     1021 acgatgcaaa cgccgttgtg caaaatttct ccctccattc tcaattccga ttggggtgat
     1081 tttcgcggtt ccattcagat gctcgaacaa cagggttgtg atctgatcca tcttgatgtg
     1141 atggatggcc actttgtgcc aaacttgacg cttggtccag atctcttgaa gcatttgagc
     1201 aaaggaacaa ccgcccgttt tgaggtccac ctgatggtct cagagcctga gaaatgggtg
     1261 gaaccttatt ttaccgacag aacagaaacc attttgattc atcctgaggg agtcccccat
     1321 gttcaccgaa ttctcgagcg aattcgggaa ttgggtgcca agccgggtat cgtactcaat
     1381 ccagggacac cgctgaatgc tttggattgg gttggcgact gcattgagca ggttcttctg
     1441 atgaccgtga accccggttt tggtggccag aagttcatcc gaggcatgtt gcccaagata
     1501 gcccaaacac gagactggct cgtccgaaaa ggtatagatg cgacgtgtcc catcgaagtg
     1561 gacggtggaa tgaatcttga gacggtgccg gaagcgctca acgcaggagc tcggatcttc
     1621 gttgtcggtg ccgcaatctt catggcggac gacccgtcaa aagcttaccg cgattttgtc
     1681 cgtctcgtca cgaaatgaca gttctctgag atgactctcg ttggaagcga tccgacgcct
     1741 gctggcgaca tcgttgagag tcgctctggg gggaagaatt tcccctgcta ttcggaaacg
     1801 ggcgtctggg cagactcgac aacaggttgc atcgcgccag gatgcaccga cggccttggt
     1861 cagcggtacg gttccaccta tcgcagtgtc gctggagcca agtctgcgtt tttcagaccg
     1921 agtcttaccc aaaccggcat ctacaaagtc tatgctgcct ggggagcggg aaatcttcgg
     1981 cagaatggga tcacctatga ggtagcccac tcgggtggta ttgccaaaca tatcattgat
     2041 cagacgatca atgccaacac atgggtgctg ttggggcagt actcgttcgt caagggcacg
     2101 gatggctttg ttgaagtcag caacaaagac attgatgtta gtggaaacat gtactccggt
     2161 gcggttcggt tcgaactggt tcaggcacag cccgatccca cttcgacacc gaccccaaga
     2221 cccaccatcg gtccgcgcac ggatggcacc tggatgatgt cgaggtagtt cagcgggcag
     2281 cgcgatcttt ctctgaaacc ggaatgccag ccctcaggcc ggggtcttcg tattggctca
     2341 gttacacaag ggcggaggct gtcattccga acgactgagg cttggcgaag gagtgaggaa
     2401 tctctctaga ttctgcagta aagagagatt cttcattccg ctcccagagc gtcgctgcac
     2461 tcggaatgac aacaatacct tgtgtaactg agctgatacg ggtctttccg tctttcgctt
     2521 gacctattct gtttcccgtc aaataatcag attatctgaa cacgctttct ctgttttgtc
     2581 gtttaacatg tattagacgg cttcagggaa gtccgggagt cccagtggat gaaggcggat
     2641 aatgagcacg aattgcttca acaatggaaa gagggcagcc aagaggcctt cggagagctt
     2701 ttctcccgaa atgcttccga tctgtacaac tttgcctacc gccttacggg acaaaccgaa
     2761 gatgccaggg acatccttca gaccgctgct ctcaaagcgt tttcccgagg gtgtccaagc
     2821 ggagaagcaa cccgtttcta cgcctggatg cgacgggtgg tacttaacct cttcctcgac
     2881 tcggagcgaa caaacagaag ggctggaagg ccgtccctgg tacaaaacgt tgactgggag
     2941 gacatcgagt tcgccgctgt cccacaaagc cagctggcca gtccacgtgc tgaaatggac
     3001 gagcaccaac gaatgcaagc catcagaaaa gctttggatg gccttccaac cgaatatcgc
     3061 gctgttgtgg ttctccggga cgtggaaggg ctctcgtatg cggaaattgc cttgcatctc
     3121 tctgtgggcg tggaaacagt tcgaacaagg cttcgtcggg cacgtgagcg gctgcgggat
     3181 accctgcagc cgctggtcag tgaagagtcc tgagagtccg accgccaaag gtgaagcacg
     3241 atgaaggaaa aagaaacgtc ctgtcgcttt cgcgacctgc tctccgacgc tctggatgga
     3301 gaactgtctg ccgttcagca agcgcaactt gaagagcatc ttctggtgtg tgaagactgc
     3361 cgaagtgcct tgagtgccta tcagaaggtg acgagcctct taagggagat gcctgaagcg
     3421 tccatgccga ccgatatggt tcaagccgtt ttgaaggaga cgacgaacac tcaggccgtc
     3481 ccggtaacaa tgcaggagaa cgacggcgtg gctgcccttg ctccaggacg acaaggttcg
     3541 agcatgcgtt gggttcgttg gagtgctgct gtggctgccg tgctcgtggt cgcgttcttg
     3601 tggaatggac tgatcagcat caacaatgtt ttatctcgtc cgatgttgac ctcaatagtg
     3661 gccagcgctg atgaggaact gtctggaata gacttggttt tctcctttaa ccccagaagg
     3721 atttctgtga aaaccattga atctgcgctg gaaagcactg gatttcttat tgtaagtcat
     3781 gtggggaagg atgaggttcg aatctcgatg gcgtcaccgg agagatttca tcccgatggc
     3841 ggtgcatcca ttgtaaccat gcagattctt ccaaagagtc tgtctcgtga tacgagttct
     3901 ttcttgcgtt tggaagcggt tcgggcatac cggccggatg gacgtcctgc ttctgtgacg
     3961 cttgaccagg tttccgtgcc gttgcccgaa cagtgcaatg gcgacggggg tacaactgcc
     4021 taatcttgag aattatggag gatagggaga tgagtgttgg taaaagagtg gttttcggaa
     4081 caggactgtt gttggttctg tgtgtggcga gtgcgatggg agtcgaaccc gtcgcgccga
     4141 ctttggtggc tcccccggac ggcttcgagg cgattgggcc c
//
LOCUS       DAOQUC010000164         4155 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_24017_length_4155_cov_3.247561, whole genome shotgun sequence.
ACCESSION   DAOQUC010000164 DAOQUC010000000
VERSION     DAOQUC010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4155
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24017_length_4155_cov_3.247561"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..899
                     /locus_tag="PKH07_04050"
     CDS             <1..899
                     /locus_tag="PKH07_04050"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNT34150.1"
                     /translation="DIQQGLQGNPQAVFICSPTDFHLEQAKMAVLGGADVMVEKPLST
                     SVEGVDGLEALASEHGKVVMVAHCFRFHEGLKKAKSLVHEGVIGRLLSIRASVGEYIP
                     DVMPDYRNMYISKLNGVYELMHDIDLALWFADQPPVRVFGIHGRFSDVGMKSPDMVEI
                     LIEFKERCLASIHLDFFQRARRRQIDLLGTKGTILVDFAHWDECLISLFRSKTQAWTY
                     KKLVTDRDDMFREENRLFLQACITREPSPLSVAQGRLPIQIIDAVKDCQQTHKAMDIV
                     LPERTMTLMGSMPLSASVHADG"
     gene            896..1918
                     /locus_tag="PKH07_04055"
     CDS             896..1918
                     /locus_tag="PKH07_04055"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNT34151.1"
                     /translation="MNGLTRITTALRCGIPDRVPLLEFIIDPTVYRAIVPEAACQADF
                     EEHFDFDAVGTGAKFRSVRENSDGTYFDEWGVLYRRTSEVISHPLRGPVECMRDLEKW
                     KGPDPDADHLYVALDSLVSRFKGQRAVFFHHRAAFMWSAYLHGIDNLLADFLAEPELA
                     HALLDKVLDINLRIIRNAIRRGADVIVLGDDYADNSGPMMSPCVFQEFLLPRLRKTVE
                     TIHREGALVVKHTDGNIWKILDDIVETGADGLNPIEPSAGMELSEVKAHCGDRICLLG
                     NIDCGHLLSKGTHEQVREAVQKCIEAGAVGGGFILTSSNSIHSSVRPENYLAMIQAAK
                     EFGSCY"
     gene            complement(1920..2558)
                     /locus_tag="PKH07_04060"
     CDS             complement(1920..2558)
                     /locus_tag="PKH07_04060"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNT34152.1"
                     /translation="MCRICFRNVHTQGLTLIELLIVVAIVGILASIAIPNFLLSQVRA
                     RVARVQADHQAIGVALELYRLDENDYPPMREPGFSGGPPAIQGSELKWWYVPNVLSTP
                     VAYLSKSEILCPFGGNYDKEPYFPDEIWKRYGYENIAEMVAKAQSGWTVFQWRYPPEA
                     IAWSGFWRLQCVGPDKIWNPSVTYDPSNGVISGGDIIRTQKKPSGNVAPGGP"
     gene            complement(2604..3521)
                     /locus_tag="PKH07_04065"
     CDS             complement(2604..3521)
                     /locus_tag="PKH07_04065"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNT34153.1"
                     /translation="MSRKPLVSVVVLTHGEGPYVADTVRSVLATDYVPLEIIVVDNEA
                     SEKTKCLIQTLPTNIRVVETGKNLGFAGGNNKGIEVATGEIVVLLNDDTRVEPEWISA
                     FVEQFQMRPNAGICGGLLLSWDGASIQHAGGIVLPNGLTWHVGQGEPFHRQYPGIRET
                     EYVTGAVFAIHRKVLDRIGLLESSYFPLYFEEVEYCRLARKVGFEVLYVPKAIVYHRV
                     METSGQFSQKYFCRYHVNRLRFLLRNYTVGEMIRAAVHELKWILSGKNRDQYAPLLKA
                     YLKTLLSLLSILQGRRRLKRIRGCRTAQN"
     gene            complement(3518..>4155)
                     /locus_tag="PKH07_04070"
     CDS             complement(3518..>4155)
                     /locus_tag="PKH07_04070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34154.1"
                     /translation="LVSVMQSDPSVALAGSRLLLPDEVNIQADAPLMLPNGLAFDPNY
                     GNPPASEEESVCTALYATGAALAARREFLELFGGFDEGYSPIYFEDVDLAFRAYRLGL
                     KTVIVRSSRVLHFESHAMGKATHRYCYLYHRGRFRCMLLHLKTTALLNSWPRSERYWY
                     RTHGRNLQPQALLEAYGHTASVLPRILLERFRLARKIKAIARRFQEAERIL"
BASE COUNT          973 a         1118 c         1112 g          952 t
ORIGIN      
        1 aagacatcca acaggggctg caggggaatc ctcaggctgt atttatctgc agtccgacgg
       61 acttccacct tgaacaggcg aagatggctg ttctgggtgg tgcagacgtt atggtggaaa
      121 agccgctgtc cacatcagta gagggcgtgg acggtcttga agccttggct agtgaacacg
      181 gcaaggtggt catggtggca cattgctttc ggtttcacga ggggctcaaa aaggcaaaga
      241 gtctggttca cgaaggggtc attggccgcc tcttgtccat tcgtgccagt gttggtgagt
      301 atatccctga tgtcatgccc gactatcgca acatgtatat ttcgaaactc aatggcgtct
      361 atgagttgat gcacgacatt gacctcgcgc tgtggtttgc ggatcagcct ccggtacggg
      421 tctttggcat ccatggccga ttcagtgatg taggaatgaa gtctcccgat atggtcgaaa
      481 tcctgatcga gttcaaagag cgttgtcttg ccagtatcca cttggatttc ttccagcgcg
      541 cgcgacgccg acagatcgat ctcctgggga caaagggcac gattctggtg gattttgcac
      601 actgggatga atgcctcatt tccctgtttc gctccaagac acaggcctgg acttacaaga
      661 aacttgttac ggacagggac gacatgtttc gggaagaaaa ccgcctcttt ctccaggcgt
      721 gcattacaag agagccctca ccactgagtg ttgctcaggg acgtctgccg attcagatta
      781 ttgacgctgt gaaggattgc cagcagacgc acaaggcaat ggatattgtc ttgcctgaac
      841 gcacgatgac actcatgggt tcaatgcctt tgtccgcctc ggtgcacgca gacggatgaa
      901 cggactgact cgaatcacaa cggcactgag atgcggcatc cctgatcgtg tccccctttt
      961 ggagttcatt atagacccaa cggtctaccg cgccattgtg cccgaggctg cttgccaagc
     1021 tgatttcgag gaacatttcg acttcgatgc cgtcggaacc ggcgccaaat tccgctcggt
     1081 gcgagagaat tctgacggca cctacttcga tgaatgggga gtactctacc gacgcacgtc
     1141 cgaagtcatc agccacccac tgcgaggacc cgttgagtgt atgcgggatc tggagaaatg
     1201 gaagggaccc gatccggatg cggatcatct ctacgtggcg cttgattcgc ttgtctcgcg
     1261 cttcaaggga caacgagccg ttttctttca ccatcgagcg gcgttcatgt ggtcggcata
     1321 ccttcacggt atagacaacc tgcttgcgga ctttcttgcg gagcctgaac tcgctcatgc
     1381 acttctggac aaagtgctcg acatcaacct gcgcatcatc aggaacgcca tccgacgtgg
     1441 tgcagatgtc atcgtcttgg gcgatgacta cgcggacaac agcggaccga tgatgtcccc
     1501 ttgcgtgttt caagagttcc tgctgcctcg actcaggaag acagtcgaga cgattcatcg
     1561 tgagggagca ctggttgtca agcataccga cggaaacatc tggaagattc tggacgacat
     1621 cgtcgagacg ggtgcggatg ggctcaatcc tattgaaccc tctgccggaa tggaattgtc
     1681 tgaggtcaag gcacactgtg gcgatcgaat ttgccttctg ggcaacattg actgcggaca
     1741 cttgctgtca aaaggcacgc acgaacaggt gcgagaagca gttcagaagt gcattgaggc
     1801 gggtgcggtt ggcgggggat tcatcctgac ctcatccaac tcgatacatt cctcagtcag
     1861 gccggagaac tatctcgcaa tgatccaggc cgcaaaagag ttcggctcct gctattgagt
     1921 caggggcctc cgggcgcgac gtttccactc ggtttcttct gtgttctgat gatatctccg
     1981 ccgctgatga caccgttgga aggatcatac gtcacggaag gattccaaat cttgtctgga
     2041 ccaacacact gaagtctcca gaaaccgctc caagcaatgg cctccggagg atatcgccac
     2101 tgaaagacag tccaaccgct ctgggctttt gcgaccatct ccgcgatgtt ctcgtagccg
     2161 taccgcttcc atatttcgtc tgggaaataa ggttccttgt cataattccc accgaaggga
     2221 cagaggatct ctgacttgga cagatacgca acaggagtgc tcaacacatt gggaacgtac
     2281 caccatttga gttcagaacc ctggatcgca ggagggccac cggaaaagcc cggttcccgc
     2341 atcgggggat agtcattctc gtccagacgg tagagttcca aagcgactcc aatggcctga
     2401 tgatcagcct ggactcgagc gactctcgca cgcacttgag agagcagaaa gtttggtatg
     2461 gcaatcgaag ccagaatacc gactatcgcg accacgatca gcagttcaat cagagtcaaa
     2521 ccttgtgtgt gaacgttccg aaagcagatt ctgcacatgt ctcaactcac aagagctttt
     2581 caatggagca tcccatccgc ccctcagttt tgcgcagtcc gacaccccct gattcgtttc
     2641 aatctccggc gaccttgaag aattgagagg agactcaaaa gggttttcaa gtaggctttc
     2701 aggagaggtg catactggtc gcgatttttg cctgaaagaa tccacttcaa ctcatgaact
     2761 gccgcacgga tcatctctcc aacagtgtag tttctcagga gaaatcggag cctgttgacg
     2821 tgatagcgac agaaatactt ctgtgaaaac tgtccgctcg tctccatgac ccgatggtag
     2881 actattgcct ttggaacata gagcacctca aacccgactt ttcgagccag acggcagtat
     2941 tcgacctcct caaaatacag agggaaatac gaggattcca gaagtccgat gcgatccaag
     3001 accttgcgat gtatcgcaaa gacggctccg gtgacatatt ccgtctctcg aatgcctggg
     3061 tactgtctgt gaaaaggttc tccctgaccc acatgccagg tcagaccgtt gggcagtacg
     3121 attccgcctg catgctggat ggatgcacca tcccaggaca gtagcagccc accgcagata
     3181 cccgcgttgg gtcgcatctg gaattgctcg acaaacgcgg atatccattc tggttccact
     3241 cgggtgtcat cgttcagaag aaccacgatc tcacctgtgg ctacttcgat tcccttgtta
     3301 ttcccgcccg caaatcccag gttctttcct gtttcgacga ctctgatgtt ggtgggcaac
     3361 gtttgaataa ggcatttcgt cttttcactg gcttcgttgt ctacgacaat gatctccagt
     3421 gggacatagt ccgtcgccag cacactccga actgtgtctg ccacataagg accttcaccg
     3481 tgtgtcagaa caacgactga gacgagtggt ttccggctca cagaattctc tccgcctctt
     3541 gaaaacgtcg cgcgatggcc ttgatctttc gggcaagacg aaaacgttcg agcaggattc
     3601 tcggcagaac actggcggta tgaccatagg cctcaagcag agcctgaggt tgcagatttc
     3661 tgccatgtgt tcggtaccag taacgctccg accgaggcca ggagttcagg agcgccgttg
     3721 tcttgagatg cagaagcatg cagcgaaacc gtccccgatg atagaggtaa caatagcgat
     3781 gcgtcgcttt gcccatcgcg tggctctcga agtgaagcac gcgagatgag cggacgatca
     3841 cggtcttcag ccctagacgg tatgcccgaa aggccagatc aacatcctca aagtagatgg
     3901 gggaatagcc ttcgtcgaaa ccgccgaaaa gttcgagaaa ctctcgccga gccgccagcg
     3961 ccgcgcctgt tgcatagagt gccgtgcaga cggactcttc ctcggaagcg gggggattcc
     4021 catagttcgg atcaaacgcc aatccgttgg ggagcatcaa tggagcatcc gcctggatgt
     4081 tgacttcgtc cggcagaagc agtctcgaac cggcaagcgc aacgctcgga tctgactgca
     4141 taaccgatac caacg
//
LOCUS       DAOQUC010000165         4146 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_24089_length_4146_cov_2.824248, whole genome shotgun sequence.
ACCESSION   DAOQUC010000165 DAOQUC010000000
VERSION     DAOQUC010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4146
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24089_length_4146_cov_2.824248"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..964
                     /locus_tag="PKH07_04075"
     CDS             <1..964
                     /locus_tag="PKH07_04075"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNT34155.1"
                     /translation="VNSPEREVTWIPPGASGPVRLNPGDADVSFSQKDVLPSLGFELK
                     PLAKVTLRGSYSQTVARQTFKELSPIQQQEYLGGDVFIGNPYLGMSSLKNYDLRLDYT
                     PYQGGLASVSYFFKDVKDPIEYVQRNAGFTYTTPVNYPNGTLSGFELELRQEVGHFWK
                     RFEGLSLGANATFISSEVTLPDDEAAGFDLPNIRAPMKSRNMTNAPEHLYNFFMSYQL
                     DTLGLKGTQLSLFYTVRGDTLVAGAGQSNGKFVPDVYELEYGTLNLSLAQKIGDNCKV
                     TFQAKNLTNPEIQKAYRSEYIGDDVVKNSYKKGMEFSLGLGFEF"
     gene            1144..>1960
                     /locus_tag="PKH07_04080"
     CDS             1144..>1960
                     /locus_tag="PKH07_04080"
                     /inference="COORDINATES: protein motif:HMM:NF023358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3450 family protein"
                     /protein_id="HNT34156.1"
                     /translation="MSGLVRQMRRTSELFGQESVKGRWCSTLRVGVLVAVALLSASDR
                     AFGSDVSSIEKARTSLEKWVETQKIISQEQRDLALAKDVLNERIQLVEREIESLRAKT
                     KETESGIAETDKKRAELVKENDTLKDASSALQEVLIVLELGTKRLLTRLPDPIRERVK
                     PLSQRIPDGSEEVSIPLAQRFQNVVGILNEVNKFNGEITVTSEVRSLPDGTSAEVATL
                     YLGLGQAYYMGGNGTIAGIGMPSEQGWQWKSTNESASAVSKALAILKNEQVASF"
     assembly_gap    1961..1970
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2001..3548
                     /locus_tag="PKH07_04085"
     CDS             2001..3548
                     /locus_tag="PKH07_04085"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNT34157.1"
                     /translation="MVKKRILIILSLCLLCAVARAQVESASEQATFSQAADSLQRRLE
                     VSISELNELRQQVAQEKIPLSRKLSDVERELAETRQKYQETTRLLDGRTLELANLKNE
                     ITARQEEATYLSGLLHEYIRSFESRLHIAESHRYKEALEQAKLASQNSNLSQREIFVA
                     EAALLKVSLERLEDCLGGTRFEASVVDSNGIVKEGMVLRVGPAALFRSKDCANVGTVE
                     QRLGSLEPAVIAFSSPSDVTAAAEIISEGTGFFPLDPTLGNAHKIEATQETFLEHVKK
                     GGPVMYPIFALAGSALLVSLYKYLSMLFIRRPSQKRVQLLLSAVAAHDKKTAEERARR
                     IGGPVGRMLATGVEHLKEPKELIEEVMYEHILSTRFDLQRFLPFIAISAASAPLLGLL
                     GTVTGIINTFKLITVFGSGDVKTLSSGISEALITTEFGLIVAIPSLLIHAFLSRKARG
                     VIVQMEKAAVAFVNQVSKTPYKDTWLSESDTASVKRHQQDKRETQEPVELLSVLAQPE
                     RSHEASR"
     gene            3564..>4146
                     /locus_tag="PKH07_04090"
     CDS             3564..>4146
                     /locus_tag="PKH07_04090"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNT34158.1"
                     /translation="MASEATRQLLQADSLANGLAFLQDFATRELNAEALRGHLMALWQ
                     QALEIWISGGWAMVAIAVISLVMFALGMHVYLSLSGKGFARVREKTWRHWINHPEERR
                     GAIGELLNFVTGGASLREIASSFEELRSTEVSPFDRDLGVMKICVGAAPLLGLLGTVT
                     GMLATFKALASGAGGEKTMEMIARGRFAFDREGR"
BASE COUNT         1001 a          991 c         1177 g          967 t
ORIGIN      
        1 cgtgaattct cccgagaggg aagtgacttg gattccgccg ggtgcctcag ggccggtgcg
       61 gctcaatccc ggtgatgctg acgtctcttt ttcacagaag gatgtgcttc cttctctcgg
      121 attcgagctg aaacctcttg cgaaagtcac gctgcggggt tcctacagcc agacagtggc
      181 acgccaaacc ttcaaggaac tcagtcccat tcaacaacag gagtacttgg ggggcgacgt
      241 ctttattgga aatccgtatc tgggcatgag ttctctgaag aattacgatt tgcgcctgga
      301 ctatacgccc taccaagggg ggctcgcctc agtctcctac ttcttcaaag acgtcaagga
      361 tccaatcgag tatgtccaac gcaacgcggg gttcacctat acaacaccag tgaactatcc
      421 aaacggaaca ctgagtggat ttgaacttga gctccgtcag gaggttggtc atttctggaa
      481 gcgttttgaa gggctctctt tgggtgccaa cgctaccttc atcagctcag aggtaactct
      541 gccggatgac gaggcagcag gttttgacct tcccaacatt cgagcaccga tgaagtcccg
      601 caacatgaca aatgctccag agcatctgta caacttcttc atgagttacc aactcgacac
      661 actcggtctg aagggcaccc agttgagtct cttctataca gtcaggggag acacccttgt
      721 cgccggagca ggacagtcaa atggaaagtt tgtgccggat gtctatgaat tggagtacgg
      781 aacactcaac ctaagcctgg cacagaaaat cggggacaat tgtaaggtca cttttcaggc
      841 aaagaatctg accaatccgg aaatccagaa agcctatcgt tccgagtaca tcggtgatga
      901 tgtggttaag aactcttaca agaaaggtat ggagttttcg ttgggacttg gttttgagtt
      961 ctagcatgac gctctttcgc cttgcgtcgc ttcgcggtgg tgactgcgga aggcttatgc
     1021 agcattttcg cccggtaccc tttgacagga atccaaccta ttgctaactc cctgctaaca
     1081 atctgcagat agcctcctta cgaaatggaa cacaaacgag agaaagagca ttgcaggaag
     1141 ccaatgagtg gtcttgttcg acagatgcgc cgaacatctg aactgtttgg acaagagagt
     1201 gtgaagggaa ggtggtgctc aacactcagg gtgggcgtgt tggttgcagt ggccctcctg
     1261 tcggcttcgg atcgtgcctt tggttcggat gtgtcgagta ttgagaaggc acgaacgtct
     1321 ctggagaagt gggtagagac gcagaagatc atctcccagg aacaacgcga tcttgcgctt
     1381 gcaaaggatg tgttgaatga aagaatacag ctggtcgagc gtgaaatcga atctcttcga
     1441 gccaagacca aagagaccga gtcgggaata gccgaaacgg acaagaagcg tgcagaattg
     1501 gtcaaagaga acgatacttt gaaggacgcc tcgtcagcgc ttcaggaagt gctgatcgtt
     1561 cttgagttgg gaaccaaacg ccttttgaca cgacttccag atcccattcg ggagcgggtc
     1621 aagcctttga gtcaacgaat tcctgacggt tcagaagagg tgagtattcc gctggcacag
     1681 aggtttcaga atgtggttgg gattctcaat gaggtcaaca aattcaacgg ggagatcacg
     1741 gtcaccagcg aagtccgttc gctgccggat ggaacttcgg cagaagtggc aactctctat
     1801 cttggccttg gtcaagccta ttacatgggt ggaaatggaa cgatagcggg aatcggaatg
     1861 ccgtcggaac aaggatggca atggaaaagc accaatgaat ctgcgtctgc tgtttccaaa
     1921 gcccttgcca ttctgaagaa cgagcaagtg gcaagtttcg nnnnnnnnnn ccgatggaag
     1981 ttcggtgagt tggtaagcct atggtgaaaa agaggattct aatcattctc agcttgtgtt
     2041 tgctgtgtgc tgtggcacga gcgcaagtgg agtctgccag cgagcaggca acattcagtc
     2101 aggctgccga ctctcttcag agaaggcttg aggtcagtat ttctgagttg aacgagttac
     2161 gtcagcaggt ggctcaggag aagattcctc tcagccgcaa gctcagcgac gtagaaagag
     2221 aactcgcaga gacgcgccag aaataccagg agaccactcg cctgctggat gggcgaacac
     2281 tggaattggc aaatctcaag aatgagatta ctgcgcgaca agaggaagca acttatctct
     2341 ccggtttgct ccatgagtac attcggagtt ttgaatcacg ccttcacatt gccgaaagcc
     2401 atcgctacaa agaggcgctt gaacaggcaa aactcgcctc tcaaaacagc aacctttccc
     2461 aaagagagat ctttgtggca gaggcggcgc tactcaaagt ctctttggag agactggagg
     2521 actgtttggg aggaacccga tttgaagcga gtgtcgtaga ctcgaacggc atcgtcaagg
     2581 aaggaatggt cttgagggtt ggccctgcgg cattgtttcg gtcgaaggac tgcgcgaatg
     2641 ttgggactgt ggagcagagg cttggctctt tggagccggc agtgattgcc ttcagttctc
     2701 cctctgatgt cacggctgcg gcagagatta tcagtgaagg gacgggtttc tttccgcttg
     2761 atcccacgct tggcaatgca cacaagattg aggcgacaca ggaaaccttt cttgagcatg
     2821 tcaagaaggg cggtcctgtc atgtatccga tttttgccct tgccggctct gcccttttgg
     2881 tgtcgttgta caagtatcta agcatgctgt tcatcagaag gccctctcaa aaaagagtcc
     2941 aattgctttt gagcgctgtc gctgcgcacg acaagaagac tgccgaggaa cgagcccgaa
     3001 gaattggcgg tcccgttgga cggatgcttg ccactggtgt ggaacacctg aaggagccca
     3061 aagaactcat tgaggaagtg atgtatgaac acattctttc gacccgattc gatctgcagc
     3121 gatttcttcc gttcattgcc atcagtgccg catcggctcc attgctcggt ttgctcggaa
     3181 cagtgacggg gattatcaac accttcaaac tcatcacagt ctttggctca ggagatgtga
     3241 agacactctc cagtggcatc tctgaggcac tgatcaccac ggagtttggg ctcattgttg
     3301 cgatcccttc cctgctcatc cacgcctttt tgtcgcgcaa ggcacgaggt gtcatcgttc
     3361 agatggagaa ggccgcggtt gcttttgtca atcaagtcag caagacaccc tacaaggata
     3421 catggctttc cgagtcggac actgcctcag taaagagaca tcaacaggac aagagggaga
     3481 cacaggagcc tgtcgaattg ttatcggttc tcgcgcagcc ggagcgatct catgaagcca
     3541 gcagatagag gaggtttggt cagatggcat cagaggcaac gagacaactc ctccaggcgg
     3601 actccctggc gaacggtctt gcattcctgc aggattttgc cacgagagaa ctgaatgccg
     3661 aagcgcttcg tggtcacctg atggccctct ggcagcaagc tttggagata tggatttcag
     3721 gcggatgggc gatggtcgcc atagcggtga tctccctggt gatgtttgcc ttgggtatgc
     3781 acgtttacct gagcctttcg ggcaaggggt ttgctcgcgt tcgggagaag acatggcgtc
     3841 actggatcaa ccatcctgaa gagcgtcgtg gtgccattgg cgaactgttg aactttgtga
     3901 cagggggagc ctctctgcgg gagatcgcct cgtcctttga ggagttgcgt tcgaccgagg
     3961 tatcgccctt cgaccgcgac ctcggtgtca tgaagatctg tgtaggggca gcacctctgc
     4021 tgggcttact gggaacagtg acgggaatgc tcgcaacatt caaagccttg gcatcgggtg
     4081 ctggtggcga aaagacgatg gagatgattg ccagggggcg tttcgctttc gacagggaag
     4141 gccggg
//
LOCUS       DAOQUC010000166         4140 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_24123_length_4140_cov_3.852632, whole genome shotgun sequence.
ACCESSION   DAOQUC010000166 DAOQUC010000000
VERSION     DAOQUC010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4140
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24123_length_4140_cov_3.852632"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="PKH07_04095"
     CDS             <1..195
                     /locus_tag="PKH07_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008277911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34159.1"
                     /translation="VQGILENPDQIRMSRKDTRVYLFYGTERPGRWICAVVKKLNGSG
                     FLITAYPTDAVKEGETVWHR"
     gene            183..395
                     /locus_tag="PKH07_04100"
     CDS             183..395
                     /locus_tag="PKH07_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2283 domain-containing protein"
                     /protein_id="HNT34160.1"
                     /translation="MAQIKVYYDRKGNTLTVWLGNPQDECICEETGDEVVLMKDAAGQ
                     VIGFERLNFSVGLSEPLGVAFEASAV"
     gene            496..1926
                     /locus_tag="PKH07_04105"
     CDS             496..1926
                     /locus_tag="PKH07_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HNT34161.1"
                     /translation="MSGIFGVVSHQNCRDLLFYGTDYHSHLGTQFAGMAVLGKGFSRQ
                     IHSIAQSHFKSKFFEDSRKMEGDKGIGVVSASDEQPIYLHSKVGPYCIVTNGQVQNRD
                     QLSRDLMKKGISFSEFREGQINTTELVAKLISQGDNLLDGIDKMFNLIEGSCSLLLLN
                     EDGVYAARDRLGYSPLVIGQRPDALAVTSETCAFPNLGLETVKFLEPGEIVLINKTLL
                     QKRPGSGGKQICTFLWIYTGFPSSDYEGINAESVRERCGQFLARRDKDIEADMVSGVP
                     SSGIGHGLGYAMESGKPFRRPLEKYTPGYGRSYIPLSQETRDFVAKMKLIPNRAIIQG
                     NRIVVCEDSIVRGTQLKNFTIRKFWDCGAKEIHIRAACPPLMFPCRFNVSTRDIEELA
                     ARRAIRALEGQDVVDLSDYLDPLSEKYKKMVDWICRDLEVTTLRYQTLDDMVAAVGLP
                     REHLCLYCWTGQCPEGSCAACGASKP"
     gene            1938..2387
                     /locus_tag="PKH07_04110"
     CDS             1938..2387
                     /locus_tag="PKH07_04110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34162.1"
                     /translation="MQENTQEQQTPEVLRWQVHPARESGLKALLLCLFLLAVLCAVQV
                     VYQKVFWTVLSALLMTSSLAGFFLPTRYEMDSSGVTVSRLWFRHHQRWDRFRSFCIDK
                     RGVLLSPFDRPNRLENYRGSFLLVHPADQPLVLECVRRHLREMERNS"
     gene            2365..2724
                     /locus_tag="PKH07_04115"
     CDS             2365..2724
                     /locus_tag="PKH07_04115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34163.1"
                     /translation="MKWNAIRDTFKHAFAVPGSEPLSSEEEALLERLAAFVVRRRLSA
                     PAAFFLTSLKPLNYVGSQAMWVLQPLAELFVAPEEFLKAQRLLERRESMELLVQRIEQ
                     LQQSQAEQRKTEGKQKG"
     gene            2731..>4140
                     /locus_tag="PKH07_04120"
     CDS             2731..>4140
                     /locus_tag="PKH07_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HNT34164.1"
                     /translation="MSDLNVIIATDCGSTTTKAILIERKGDEYRQTYRGEAPTTVEAP
                     HNDVTRGVLNAIAEVEDLSGRQILDGERIITPAEGNRGVDIYISTSSAGGGLQMLVAG
                     AVKTMTAESAQRAALGAGAIVMDVLASNDGRLPHEKISLIRGLRPDMILLSGGTDGGT
                     VSHVVELAEFIAAADPKPRLGHTFRLPVIFAGNKNARPEIENTLGEKTDLAITDNIRP
                     TLEQENLFPARMKIQDLFMEHVMAQAPGYGRLKSWTSVPIMPTPAAVGNIIRTVSEQE
                     GINVLGVDIGGATTDVFSVFDEVFNRTVSANLGMSYSVSNVLAEAGLANVLRWVPFEM
                     DEEDLRNRIKNKMIRPTTIPQTLQELIIEQALCREALRLAFIQHKLLAVGLKGVQQER
                     TISDLLQQSEAGATLVNMMSLNLIVGSGGVLSHAPRRRQSMMMMIDAFQPEGVTRLAV
                     DSIFMMPHLGVLAEVDKRAA"
BASE COUNT          937 a         1036 c         1227 g          940 t
ORIGIN      
        1 gtccaaggca tacttgaaaa tcctgaccag atcaggatga gccgaaaaga cactcgtgtc
       61 tatctgttct atggaacaga gagacctggc agatggatct gcgctgttgt caagaaactc
      121 aacgggagtg ggttcctgat aacagcgtat ccaacggatg ctgtcaagga aggagagacc
      181 gtatggcaca gataaaggtg tattatgatc gtaaggggaa cacgcttact gtgtggctcg
      241 gcaatccgca ggatgaatgt atctgcgagg agaccggtga tgaggtcgtt cttatgaagg
      301 atgcggccgg tcaggtcatt gggtttgaaa gactcaattt ctccgttggt ctttccgaac
      361 ctttgggtgt tgctttcgaa gcgtcagccg tctgaacgct cgcactatgg cacaatcgca
      421 gatgcgactg ttgaacgatc atccctgaga ggtaacattt tttggggctt agtagccctc
      481 ttctggaggc atttgatgag cggtattttt ggcgttgtct cgcatcagaa ctgtcgcgat
      541 ctgttgttct atggaaccga ttaccattcg catctgggaa cgcagtttgc agggatggcg
      601 gtcttgggaa aaggattctc gcgccagata cacagcatcg cgcagagtca cttcaaatcc
      661 aagttcttcg aagattcgcg gaagatggaa ggggacaagg gcatcggggt ggtgagcgct
      721 tccgacgagc agccaatcta ccttcattca aaagttggcc cgtactgcat tgtcaccaat
      781 ggccaagttc agaaccgcga tcaactgtca agagacttga tgaagaaagg aatctccttc
      841 tccgagtttc gggagggaca gatcaacacg acggagctgg tcgccaaact gatcagccag
      901 ggagacaacc tgctggacgg gattgacaag atgttcaatc tcatcgaagg ctcctgttcc
      961 ttgctgctgt tgaacgagga cggggtttac gctgccagag atcggcttgg gtactctccc
     1021 ttggtgatcg ggcaacgacc tgatgccctg gcggtgactt ctgagacctg tgcgttcccc
     1081 aatctgggac ttgagacagt caagttcctc gaaccgggag agattgtcct gatcaacaag
     1141 acgttgttgc agaagcgtcc tggttccggg ggcaagcaga tctgcacctt tctttggatt
     1201 tacactggct ttccgtcctc ggactatgaa ggcatcaacg cggaatccgt ccgagaacgc
     1261 tgtggacagt ttctcgccag gcgggacaaa gacatcgagg cagatatggt ctccggcgtt
     1321 ccctcgtccg gcatcggaca cgggctgggc tatgccatgg agtccggaaa accgtttcgg
     1381 agaccccttg agaagtacac tcctggctac ggacgcagct atattcccct ttctcaggag
     1441 acccgggatt tcgtcgccaa gatgaaactc attcccaaca gagccatcat ccaaggcaac
     1501 cgaattgtgg tctgtgagga ctccatcgtt cggggcacgc agctgaagaa tttcaccatt
     1561 cgaaagttct gggactgtgg cgctaaggag attcacattc gcgctgcctg cccgcccctg
     1621 atgtttccgt gtcgattcaa cgtttccaca agggacattg aagaactggc ggcgcggcgt
     1681 gctattcgag cgctggaagg ccaggatgtt gtggatctgt cggattatct ggatccctta
     1741 tccgagaagt ataagaagat ggtggactgg atttgtcgcg acttggaggt caccacgctg
     1801 cgctatcaga cgctggacga tatggttgcg gcggtggggc ttccccgaga gcatctttgt
     1861 ctttactgct ggacgggtca gtgtccggaa ggctcgtgtg cggcctgtgg cgcgagcaaa
     1921 ccttagtgct tgaggtcatg caggaaaaca cacaagaaca acagacgcct gaagtgttgc
     1981 gatggcaggt tcatcctgcg agggaaagcg gtctgaaggc tttgttgttg tgcctgtttc
     2041 tgcttgcggt gttatgtgcc gttcaagtgg tctaccagaa ggttttctgg acagttctca
     2101 gtgctcttct catgacaagt tctctcgcag gattctttct gccgacacga tatgagatgg
     2161 attcgagcgg cgtgactgtc tcgcggttgt ggtttcggca tcatcagcgg tgggatcgat
     2221 ttcggagttt ttgcattgac aaacgcgggg tgctgttgtc accttttgat cgcccgaaca
     2281 ggctggagaa ctatcgaggc agttttctgc tggtgcatcc ggcagatcaa ccgttggtct
     2341 tggagtgtgt gaggagacat ctgcgtgaaa tggaacgcaa ttcgtgatac cttcaagcac
     2401 gcattcgcag tgcccggctc agagccgctg agttcggaag aggaagcgtt gctggaacga
     2461 ctggccgctt ttgtggtgcg tcggagactg tccgcaccgg cggcgttctt cctcaccagc
     2521 ctcaagccac tcaactatgt gggaagccag gcgatgtggg tgcttcaacc cttggcggag
     2581 ctgttcgtcg cccctgagga gtttttgaaa gcacaaaggc tgctcgaacg tcgggagagt
     2641 atggagttgt tggtgcagcg catcgagcaa ctgcagcaga gtcaagccga acaacgcaag
     2701 accgagggaa agcaaaaagg ataggcgacc atgagcgatc tgaacgtcat cattgccacc
     2761 gattgtggca gtaccacgac caaggcgatc ctgatcgaga ggaaaggcga cgagtaccgt
     2821 cagacctacc gaggcgaagc ccccaccacg gtggaagcac ctcacaacga cgtgacacgg
     2881 ggcgtgttga acgcgattgc cgaggttgag gatttgtccg gacgacaaat cctggatggt
     2941 gaacgcatca tcactcccgc cgaggggaat cgcggcgtgg acatctacat ctccacttcc
     3001 agcgcagggg gtggactcca gatgctcgtc gcgggtgcag tcaaaaccat gaccgcggag
     3061 agtgcgcagc gggcagcgct tggagccgga gccattgtaa tggatgttct ggcttctaat
     3121 gatggacgcc ttccccatga gaagatctcc ctgattcgcg gtctgagacc ggacatgatt
     3181 ttgctctccg gcggcacgga cggcggaaca gtctcacacg tggtggaact cgcagagttc
     3241 attgccgccg cggatcccaa accgcgtctg ggtcacacct tccgactgcc ggtcatcttc
     3301 gcgggaaaca agaacgctcg accggagatc gaaaacacgc tgggtgaaaa gaccgatttg
     3361 gcgatcactg acaacatccg tcccactctg gagcaggaga acctgttccc ggcacgaatg
     3421 aaaattcagg atctgtttat ggaacacgtc atggcacaag cgccaggata cggcagactc
     3481 aagtcttgga catcggtgcc aatcatgccc acacccgcgg cggtcggaaa catcatccga
     3541 accgtcagcg agcaagaggg catcaatgtg ttgggtgtgg acatcggggg cgcaacgaca
     3601 gacgtgttct ccgtgtttga tgaggtgttc aatcgtacgg tcagtgccaa tctgggtatg
     3661 agttacagtg tctccaacgt tttggcagag gccggactgg cgaacgttct gcgttgggtg
     3721 ccgttcgaaa tggatgaaga ggatcttcgc aatcgaatca agaacaagat gatccgaccc
     3781 acgaccattc cccaaacgct ccaggaactc attatcgagc aagcactgtg tcgtgaagcg
     3841 ctgcggctgg cattcattca gcacaaactt cttgctgttg gtctcaaggg agtgcaacag
     3901 gagcgcacca tttccgattt gctgcagcag agcgaggccg gtgccacatt ggtgaacatg
     3961 atgtctctga atctgattgt tggaagtggc ggtgtgctct cccacgcacc gagacgccga
     4021 cagtccatga tgatgatgat cgacgccttt cagcctgagg gtgtgacacg cctggcagtg
     4081 gacagcatct ttatgatgcc tcatctgggc gttctggctg aagtggataa gcgggctgca
//
LOCUS       DAOQUC010000167         4126 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_24250_length_4126_cov_3.167772, whole genome shotgun sequence.
ACCESSION   DAOQUC010000167 DAOQUC010000000
VERSION     DAOQUC010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4126)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4126)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4126
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24250_length_4126_cov_3.167772"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1443
                     /locus_tag="PKH07_04125"
     CDS             <1..1443
                     /locus_tag="PKH07_04125"
                     /inference="COORDINATES: protein motif:HMM:NF027224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BNR-4 repeat-containing protein"
                     /protein_id="HNT34165.1"
                     /translation="KTVASLSGKSSDTVTLYYTIPRSATESVCHLRVNPSGDSSFTTY
                     STVVACSGEAPVSRPLDMPLTLKPYQHENELLGYLPDYPTESQIYFDLHNNPFVLTHQ
                     GLWTHEEGHWNVTPLVAADCSEPSLKGNTFVAMSGKVAFDKRNNIYVLAKSGNAAALL
                     YSFDRGKTFSAAMIPGYETHSRTFDIEQFSGTNIPDGPPPVLRRAKMSHDVRFKWRQM
                     YNLDLFLPTRKGDRIEMSEPILVSELCLGQAAHSGTPSALVSRGSKVHIVWGEASDPA
                     IEVPGVPGYVNTYDRKTRSLGKPVLLGYGPPANDGHNSPSITMDKAGFLHVVLGTHGK
                     PFPYTQSLKPNDTQSGWSEVRTTTEYLRQTYIGLVCDRTGTLHLVFRLWQTGEPFPNS
                     YHATLAYQQKRPGEDWQAPKTLVVSPLSDYSVFYHRLTIDRQDRLFVSYDYWSTYWFY
                     RNDHYGTRRALMMSPDTGATWKMVETSDLR"
     gene            1451..2899
                     /locus_tag="PKH07_04130"
     CDS             1451..2899
                     /locus_tag="PKH07_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015844857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNT34166.1"
                     /translation="MPDSRPNILWICSDQQRWDTLGCYGNPLVASPTLDRLAETGVIF
                     DRAFSQSPVCTPSRTAFLTGRYPRTARGNRNGADIPDYEVLVTRMLADAGYRCGLSGK
                     LHLSACHPSACPEMERRIDDGYTDFHWSHHSMGGWGLNNEYWRWLDEHGSRYQTPEHP
                     QCKYIMTGMPQELHQTTWCVEKAMDFIMARHRDGQPWLFSVNMFDPHHPFDPPESFLN
                     RYLERLDEIPLPNYVEEELANKPVYQKVDHEGAYANKGQFAYDEMTEKDHRMVRAAYW
                     AMCDLIDTQVGRLLALLEETGQSDNTVVIFTSDHGEMLGDHGIYLKGPHFYDCAIRIP
                     LILSWKKKFPARHASALVELVDLPQTLLDCVGLPKHPGMQGKSLYPLLCGETGADRHR
                     DDIYCEYYQAMPGHQRPSANLTMVRTAAHKLVVDHSHGGGELYDLAADPNETRNLWDN
                     SDLASVKSELLLRMCHRMADTVDPLPQRRAGF"
     gene            complement(3484..>4126)
                     /locus_tag="PKH07_04135"
     CDS             complement(3484..>4126)
                     /locus_tag="PKH07_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HNT34167.1"
                     /translation="AKKELEEKKKEIKSLEIGASPDTRLEPPPPRPARLRKPVVVYGT
                     SIAQGGCASRPGMVWSAILGRLLDRPMINLGFSSSGTMEPPVGEVLAEIDASAYIIDC
                     LWNIGNTSQEDYTHRVTHLVEAIRKVHPLTPIVFVGQSLLQPEKHPTEMTRKQETAVL
                     NLKKQGVKNLFLVPGTHLIGDDGEGTVDGVHLTDIGMQRQAKSLFPVLRKLLR"
BASE COUNT          949 a         1086 c         1098 g          993 t
ORIGIN      
        1 aaaactgttg catccctgag cgggaagagt tccgatacgg tgacgctgta ctatacgatt
       61 ccccgatccg cgaccgagag cgtctgtcat ctgcgggtca atccttcggg tgactcctct
      121 ttcaccacct actccaccgt tgttgcttgc agtggcgaag cgcctgtctc aagaccgttg
      181 gatatgccat tgacgctgaa gccttaccag catgagaacg aactgctcgg atatcttcct
      241 gactatccga cagaaagtca gatctacttc gatctgcaca acaacccatt cgtgttgacc
      301 catcaaggac tctggacgca tgaggaggga cactggaacg tgacccctct ggtggcggcc
      361 gattgttccg agccatctct caaggggaac acttttgtgg caatgtccgg taaggtcgct
      421 tttgacaagc gcaacaacat ttacgttctg gcaaagtcag gcaacgctgc tgcgctcttg
      481 tactcgtttg accggggaaa gacattctcc gcggccatga ttccgggcta tgagacgcac
      541 tcgcggacct ttgacatcga gcaattctcg ggcacgaaca ttccagacgg tcccccgccg
      601 gtgctgcgtc gcgctaagat gtcgcacgac gtccgcttca aatggcgtca gatgtacaat
      661 ctggatctct tccttcctac gagaaaggga gatcgcatcg agatgagtga gccaattctg
      721 gtctccgaac tgtgcctcgg tcaggccgcc cattcaggga cgccttcagc gcttgtctct
      781 cgaggttcga aagtgcatat cgtctggggt gaggcgagtg atcctgcgat tgaagttcca
      841 ggggtgccgg gctatgtgaa tacctatgat cgcaagacgc gctcgttggg caaaccggtt
      901 cttcttggat atgggccacc agccaatgac ggtcacaact cacccagcat cacaatggac
      961 aaggcggggt ttttgcacgt tgttttgggc acacatggga agcccttccc ttacacacaa
     1021 tcactcaaac ccaacgatac gcagtcaggc tggagtgaag tacgcacgac aacggagtat
     1081 ctgcgtcaaa cttacattgg gcttgtatgc gaccgtacgg gaacactgca ccttgtcttt
     1141 cgtctgtggc agacgggaga gccatttccc aacagttacc atgccacact ggcatatcag
     1201 caaaaacgcc ctggagaaga ctggcaggct cccaaaaccc tggttgtttc tccgttgtca
     1261 gattacagtg tcttctatca tcgcctgacc attgatcgcc aggatcggct gtttgtgtcc
     1321 tatgactact ggtcaacgta ttggttctac cgcaatgatc actatggcac ccgccgcgcg
     1381 ttgatgatgt ctccggacac aggagcgact tggaagatgg tggaaacgag cgatttgagg
     1441 tgataaaccg atgcccgatt ccagaccgaa tatcttgtgg atttgtagcg accagcagcg
     1501 ttgggacaca ttgggatgct atggcaaccc tcttgtggcg tctccgacat tggatcgtct
     1561 tgcagaaaca ggcgtcatct ttgatcgcgc tttctcccag agtccggttt gcactccgag
     1621 tcgcaccgcc tttctcaccg gaagatatcc tcgaaccgcc cgaggcaacc gcaatggcgc
     1681 ggacattcca gactacgagg tgctggttac cagaatgctt gccgatgccg gatatcgctg
     1741 cgggctgtcg gggaagctgc atctctcggc ttgtcatcca agcgcttgtc ccgaaatgga
     1801 acgacggatt gacgatggat atactgactt tcactggtcg catcactcga tgggtggctg
     1861 gggcttgaac aatgagtatt ggcgttggtt ggacgaacac ggaagcagat accaaacacc
     1921 agaacatccc caatgcaagt acatcatgac tggcatgcca caggaactcc atcagaccac
     1981 ctggtgtgtt gagaaagcca tggatttcat catggcgcgc catcgcgatg gtcagccgtg
     2041 gctattttca gtgaatatgt ttgatccgca tcatcctttt gacccgccgg agagtttctt
     2101 gaatcgctat ttggagcggc tcgatgagat ccctcttccg aactacgtcg aggaggaact
     2161 ggccaataag cccgtctatc agaaggtgga tcatgaggga gcttatgcaa acaaaggtca
     2221 gttcgcatac gacgaaatga cagagaagga tcatcggatg gttcgggcgg cgtactgggc
     2281 gatgtgtgac ctcattgaca cccaggtcgg aaggcttctg gccttgctgg aagagacagg
     2341 acagagcgac aatacagttg tcatcttcac atccgatcac ggcgaaatgc tgggcgatca
     2401 cggaatctat ctcaaaggac cccatttcta tgactgcgct attcggattc ctttgattct
     2461 ctcctggaaa aagaagttcc ctgctcggca tgcctccgcc ttggtggaat tggtggactt
     2521 gcctcagact cttctggact gcgttggtct ccccaaacat cccgggatgc agggtaaatc
     2581 actctatcct ttactgtgtg gagagaccgg agcggatcgg catcgggacg acatctactg
     2641 cgaatactat caagccatgc cgggacatca aaggccttcc gcgaatctga cgatggttag
     2701 aaccgccgcg cacaaactgg ttgtggatca cagccacggg ggtggggagt tgtatgacct
     2761 ggctgctgac ccgaacgaga cacggaatct ttgggacaat tcggatctgg caagcgtgaa
     2821 atctgaattg ctcctgcgga tgtgccatcg gatggcagat accgttgacc cgttgcccca
     2881 aagacgggct gggttctgat gcttcctggt cttggctgct ttcaaatgca cccattcttg
     2941 acaacctcgg tgaggttgga tagctttccg atatgaagtc aagtagttca atcatatcgt
     3001 tcatttcgag aagatgagag ggttcaacgc agtgaatctc ctccaaggct atggtgccat
     3061 cgagattcac tctcccaagg aagtggtgtg acatgaagaa gaaacagttc gactgcgtcg
     3121 agatgaagaa cgagatacaa gcccagagaa ggaaagagta tcaaggactg acagactctc
     3181 aaatccagtg tcaaatcagg ggaaaactcg cttcatccga cgatcccata gcgcgatggt
     3241 ggcgttcact tcctaaagaa agacaaggta ttccgtccca tagatcacgg tagatgttcg
     3301 gggtgttgtc agatcccatc gaaagcagcc ggggcgttta tctttttccc aaggcgagca
     3361 ttgaccttcc accctccatc ctgagcaggt tatcacctgc gggcgtgcgg gaaatgcaca
     3421 ctcttttgga cgcctgctca gtgaaagaga agtctcctcg gactgagtgt tgtcagaagt
     3481 ttgctatcgg agaagtttcc ttagaacggg gaaaagactc ttcgcctgcc gctgcatccc
     3541 aatgtccgtc agatgtactc cgtccacggt gccctctccg tcgtcgccga tgagatgagt
     3601 gcccggaacc aggaagaggt tcttgactcc ctgttttttc aagttcagta ccgcggtctc
     3661 ctgttttcgt gtcatctccg ttggatgttt ctcaggctga agaagagact gtcccacgaa
     3721 cacgatgggt gtgagaggat gaacttttcg gattgcctcc accaaatggg tgacacgatg
     3781 cgtatagtct tcctgcgagg tgtttccaat gttccagagg cagtcaatga tgtaagccga
     3841 cgcgtcaatt tctgccagaa cttcgccaac tggtggctcc atcgttcctg atgacgaaaa
     3901 gcccagattg atcataggtc tgtccagcag tcttcccaag attgcagacc ataccattcc
     3961 aggtcgtgaa gcacacccac cctgtgcgat ggatgttccg tagacgacaa caggctttcg
     4021 cagacgtgca ggcctcggtg gcggcggttc cagacgagta tcgggacttg cgccgatctc
     4081 aagggatttt atctcttttt tcttttcttc aagctctttt tttgct
//
LOCUS       DAOQUC010000168         4111 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_24384_length_4111_cov_2.274901, whole genome shotgun sequence.
ACCESSION   DAOQUC010000168 DAOQUC010000000
VERSION     DAOQUC010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4111
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24384_length_4111_cov_2.274901"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(443..1108)
                     /locus_tag="PKH07_04140"
     CDS             complement(443..1108)
                     /locus_tag="PKH07_04140"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HNT34168.1"
                     /translation="MSDTVLVVAAHPQDEILGVGGTIAKHTRNGDKVSVVLMSEGAGE
                     ELTEQRVSVLRKEAEAAASILGVEKVFRLSFPYMCFNAIPMIELTRDLYEVVNQVAPD
                     IVYTHHRGDIHTDHQMVWSATMVVTRSFGKKIVKKILSYEDPSCTRWAPPFLEYAFIP
                     NCFEDITDTIDIKMKALEAYESEHRAYPHPRSAEALRLVAEVWGIKISAGPTEAFELV
                     RSL"
     gene            complement(1194..1859)
                     /locus_tag="PKH07_04145"
     CDS             complement(1194..1859)
                     /locus_tag="PKH07_04145"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HNT34169.1"
                     /translation="MRILIIGAHPDDEVLGAGGTLARLAAQGHQVNALLLCESSSPRY
                     GDSMIPVLKEKARLSADILGIREVIFSDFSNVRTNSIPMLAMHKAITEALDHFKPNWI
                     FTHHRADVHIDHQMVFKATIEATRSFGGSFVEQLLCYEVPSSTEWAPPFPETAFLPNV
                     FYDIASTIGLKTKALSVYKSELREYPHPRSLKAIYKYGLRWGLRIGLESYVEPFEMIR
                     DIR"
     gene            complement(2081..3433)
                     /locus_tag="PKH07_04150"
     CDS             complement(2081..3433)
                     /locus_tag="PKH07_04150"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HNT34170.1"
                     /translation="MKDQDEILSLLTETLKEAPNGSECDILFVAHQESLTRFTQSAIH
                     QNLVVDNARAVVRVILDGGIGVAATNEMIPEALSAAVQKATEIAREQTQPEEVVSLPT
                     PSPIGVISTYDERTACFGPSDRAASLVRLFNQAGNHRLTLSGAFSTSAGAVAVANSKG
                     IHLFQPVTSASLNLLAFSHNGSGYASAIGRSIADFNPMDLAETAVEKCLQATDPKEIE
                     PGEYDVLLEPQAVGDLLEWTSYIGFGAREFQEGTSFMAERLGQRIAGDNITIRDDALD
                     PRGLPFPFDLEGTPKEKVTLINRGVASAVVYDRQTAAREAKQSTGHSLGAELPNRPMA
                     LNIVFETGTSTFREMLGQLERGLLVTRFHYINGYLDTRNALLTGMTRDGTFWVERGKP
                     QYGVKNLRFADSMLRVLSNVVAVGSEARAVSFGDDLLGAAVCPAILVKGFRFTSGTDH
                     "
     gene            complement(3430..>4111)
                     /locus_tag="PKH07_04155"
     CDS             complement(3430..>4111)
                     /locus_tag="PKH07_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012003571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HNT34171.1"
                     /translation="HPIELDRVLGTELSLAGGSFLTLERQGHFRYGSPIVHITADATI
                     PGGRGSFGYDDEGVPAQRYDIVKDGIFVGYLSSRETAPIIGATSGGAMRASGWNCIPL
                     IRMTNVNLEPGKGSLKDLISDTKKGLLMDSNRVWSIDDRRMNFQFGCEIAWEIKNGKL
                     GRVLKNPAYRGITPEFWSRCDAICGTEDWRVWGISNCGKGEPMQTIGTGHGAAPCRFN
                     SVHVGAEK"
BASE COUNT          952 a         1124 c         1087 g          948 t
ORIGIN      
        1 atagtccctg gttcccgcga gtggcccccc gccgggcgcc tggactgtac cttggattag
       61 gatcgtcggt ggcgtggacg ggagaatctg aggaccccca aacgcctgac tcgctgagac
      121 aactacgacc acgaatagaa gacaagaact ccgcatcatc ttgttgtcct ccttggcttg
      181 cctgggacga tgccccaagc cgcgtgctat cgaaagacct atggcttgac ttagtgaaaa
      241 atatgttact ctgatttggg aagaaaggca aatctgactc agaacatgag ctgacaagtg
      301 tgttgcggga cccgaaatcc cgactcgtgt gggagaagac tgggatgaag gcaacggagt
      361 gggagcatcc tactcccgaa tacagaatgt gtgcgggatg gcggcacatc cagggcttaa
      421 gtcacttgag gcgaaccatg aactacagcg atcggacgag ttcgaaggct tcagtgggcc
      481 ctgctgagat cttgatgccc cagacttccg cgaccagccg caatgcttct gccgagcgtg
      541 gatggggata tgctcggtgt tcactctcgt atgcttcaag cgccttcatc ttgatgtcaa
      601 tggtatcggt gatgtcctca aaacagtttg ggataaaggc gtattcgagg aagggtggcg
      661 cccatcgagt gcacgagggg tcttcgtaag agagaatctt cttgacaatt ttctttccga
      721 acgagcgggt cacaaccatg gttgcactcc agaccatctg atgatcggtg tggatgtcac
      781 cccggtggtg agtgtaaaca atgtcaggag cgacctggtt gacgacttcg tacaagtccc
      841 ttgtgagttc aatcattgga atggcgttga agcacatata cgggaaagac aacctgaaga
      901 ctttctcgac gccaagaatt gaggccgcgg cctcggcttc ctttctcaaa acagatacgc
      961 gctgttcggt cagttcctca cccgcgcctt cactcatcag gacgacagaa accttgtcgc
     1021 cattgcgtgt gtgtttggct atcgttccac ccacgccaag gatctcgtcc tgaggatgtg
     1081 ctgccaccac cagtactgta tcactcatct ttgagccatc tccttacaaa caggccatca
     1141 ctcacccaag tccgacggga cagactctcc gcttggcacg aatgcagatc cgttcatcga
     1201 atgtcccgga tcatctcaaa aggctccacg taactttcca gtccgatgcg cagtccccaa
     1261 cgaagtccgt acttgtagat tgccttcagc gatcgggggt gcggatactc ccgcagctcg
     1321 ctcttgtaaa cggaaagcgc cttggtcttc aaaccgatgg tgctggcaat gtcatagaaa
     1381 acgttcggca gaaaagccgt ctctgggaat gggggcgccc attcggttga ggagggcact
     1441 tcgtagcaca acagttgctc gacaaaggag ccgccaaacg aacgggttgc ctcaatggtc
     1501 gctttgaaca ccatttgatg gtcaatgtga acgtccgccc gatggtgggt gaaaatccag
     1561 ttgggtttga agtgatccag agcctcggtg attgccttgt gcatggccag cattggaatc
     1621 gagttggtgc ggacgttgga gaagtcgctg aagatgacct ctcgaatccc aagaatatct
     1681 gcagacagcc ttgccttttc cttgagaaca gggatcatgg aatcgccata gcgtgggctt
     1741 gacgactcgc agagcagcag ggcgttgact tgatggcctt gtgcggccaa gcgggcaagg
     1801 gttcctcccg ctccaagaac ttcatcgtcg gggtgtgctc cgatgataag aatacgcacg
     1861 gcagatttcc ttcgccaatg ccgactcgca agtcggacgt tcaacagcag cgaaaatact
     1921 ccgttgctta atccttgcta acctagaata taatgtctca aatgtcaagt gaagttgaaa
     1981 gaagatctat ctaaaacaat aaatatgaat aaattagatg cagtcaatcg ttggtgtaat
     2041 tacgacagac aaggatggtc ataccatcgg ttcagcgagt ctagtgatct gttccggaag
     2101 tgaaccggaa tcccttgacg agaattgccg gacagactgc agcgcccaga aggtcgtcgc
     2161 cgaaagaaac cgccctcgct tctgagccca ctgcaactac gttggacaga acgcgcaaca
     2221 tgctgtccgc aaagcgaagg ttcttcactc cgtactgtgg cttgcctcgt tccacccaga
     2281 acgtcccgtc gcgtgtcatt ccagtcagta acgcattgcg tgtgtccagg tagccgttga
     2341 tatagtgaaa gcgagtgact aacaagcccc tttccagctg ccccaacatc tcccgaaatg
     2401 tgcttgtgcc agtttcaaaa acgatattga gtgccattgg tcggttcggg agttccgctc
     2461 ccaatgaatg gccggtggac tgtttggctt cccgagctgc ggtctgacga tcgtacacta
     2521 cagccgatgc caccccacgg ttgatcagag tcactttctc cttcggtgta ccttcaaggt
     2581 caaaggggaa gggcagccct ctcggatcca gagcatcatc tcggattgtg atattgtctc
     2641 cagcgattct ctgacccaac ctttccgcca tgaaactggt gccttcttgg aattccctgg
     2701 caccgaatcc gatgtaactt gtccactcca gcaaatcacc aaccgcttga ggttccagaa
     2761 gaacgtcgta ttcacccggt tcgatctctt tggggtctgt tgcctggaga catttctcta
     2821 cggccgtctc cgccaagtcc atcgggttga aatcagcaat actgcgacct atcgcggagg
     2881 catagccgga tccgttatgt gaaaaagcca gcagattcag acttgccgaa gttaccggtt
     2941 ggaacagatg gatccctttc gagttggcca ctgccactgc cccggccgat gttgagaatg
     3001 cgccggaaag agtcagcctg tggttgccag cctggttgaa aagccgaact agcgaggcgg
     3061 cgcgatccga gggaccaaaa catgctgttc gttcgtcgta ggtgctgatg accccgatcg
     3121 gagaaggtgt cggaagcgaa acgacttcct ctggctgggt ctgctcacgg gcgatttcgg
     3181 ttgctttctg aaccgcggca gaaagagcct ccggaatcat ctcgtttgtg gcagccaccc
     3241 caatgccgcc atcaaggatg actcgaacga cagcacgtgc attgtctaca acaagattct
     3301 gatggatggc cgactgcgtg aagcgagtca ggctctcctg atgtgcgaca aagaggatgt
     3361 cacactcgga tccattgggc gcttctttga gcgtctcggt caacagggaa agaatttcgt
     3421 cttggtcttt catttttccg cccccacatg aacggaatta aagcggcacg gagccgctcc
     3481 atgtcccgtc cctatagtct gcatcggttc gcccttacca cagttggaaa tgccccaaac
     3541 gcgccagtct tcggttccac agatcgcatc gcagcggctc cagaattccg gagtgatgcc
     3601 gcgataggcc ggattcttca gaacacgacc cagctttccg ttcttgatct cccaggcaat
     3661 ctcacaccca aactggaaat tcatccggcg gtcatcaata gaccagactc gattgctgtc
     3721 catcagcaga cccttcttgg tatccgagat caagtccttc aaactgcctt tccctggctc
     3781 cagattgacg ttcgtcatgc ggatcaaggg aatgcagttc catccggaag cccgcattgc
     3841 gccaccgctt gtggctccaa tgatcggagc cgtttcgcgc gaactcaggt atccgacgaa
     3901 gataccgtcc ttaacgatgt catacctctg tgcgggaacg ccttcatcgt cgtagccaaa
     3961 actgcctcgt ccgcctggaa tcgttgcatc ggcggtgata tggacgatgg gagagccgta
     4021 ccgaaagtgt ccctgccttt ccagggtgag aaaacttccg cccgcaaggc tcagttcggt
     4081 gcccagcact ctatccagtt caatgggatg t
//
LOCUS       DAOQUC010000169         4103 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_24447_length_4103_cov_3.399457, whole genome shotgun sequence.
ACCESSION   DAOQUC010000169 DAOQUC010000000
VERSION     DAOQUC010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4103
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24447_length_4103_cov_3.399457"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..403)
                     /locus_tag="PKH07_04160"
     CDS             complement(<1..403)
                     /locus_tag="PKH07_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNT34172.1"
                     /translation="MRIIYFDIDCLRPDHLGCYGYNRPTSPTIDAIAKEGICFDHYYC
                     ASSPCVPSRTCLTSGRFGVRNGVVSNVGPGARFRITTRDYGGPHDANQMFPRQLKKHG
                     YDPVCFSNFADRHYALWFMCGWSEFHTPNLKS"
     gene            complement(417..1829)
                     /locus_tag="PKH07_04165"
     CDS             complement(417..1829)
                     /locus_tag="PKH07_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020418671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HNT34173.1"
                     /translation="MSDRPNILLITADDMNWDAVGAYGCPVSGTTPNIDRLASEGVRF
                     NHAHVTIAVCQPSRSVLMTGRYPHRCGGEGFFHLRTAGIPILPELLRNAGYRVGILGK
                     VEHSTPYADFKWDYTQDVGKLGKGRNPALYRQHARAFVDQAQASGKPFFLMANSHDPH
                     RPFYGNDPAEWYQPGQTPPAAVPSRTFDPQSVTTPGFLADLPEVRLEISEYYSSVRRA
                     DDTVGAILDVLRETGREQNTVVVFLSDNGMAFPFAKTNCYLHSTRTPWIMRWQGVIHP
                     GSLDSEHCISGIDLMPTLLDMVGIDPPDSMDGRSFLPVLRGQKQANRNLVFTQFHQTA
                     GRRNYPIRCVQDRQFGYVFNPWSNGSRVFVNESQDGRTMKAMLAAGENDARIAGRVEL
                     FLHRVPEEFYDFQNDPDALRNLVGVPRYRKDLQRLRRELQRWMQRTNDPALEAFENVD
                     SFETQEKLMNDYISLLGGRS"
     gene            2221..3273
                     /locus_tag="PKH07_04170"
     CDS             2221..3273
                     /locus_tag="PKH07_04170"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNT34174.1"
                     /translation="MDKVKLGVIGCGVISASHLDLANKCPMVEVVAVADLIPERAHSR
                     AQTFGVPTVYYNDEDLLNDERIEAVVLAMPAGVRTPVAFKALERGKHVLLEKPVASNV
                     HEVEKMIAMRRDRVVGCCSSRMTFSGHAQTAAACVRSGVLGKIRLLRIRAVHPAGAQP
                     NPEPPPWRESMQLNGGGILVNWSCYELDYLMHIVGWQICPKAVMAQWWPVAEKMSAYV
                     AAGSDADAHYTAMILCNDGVALSMERAEFSSISADCAWEIIGTDASLHLPMHIRKGVP
                     NSVVLDRFVPGEGIVSETLWEPGQGDPDENVITDFVQAIQEHREPRTNLERALVLQKI
                     TNGIYGSAASGSSVAV"
     gene            complement(3394..>4103)
                     /locus_tag="PKH07_04175"
     CDS             complement(3394..>4103)
                     /locus_tag="PKH07_04175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34175.1"
                     /translation="HMDNGTDVFGSIEAVGALTGGARVNGLNVDGQPNTMTTGGDIAD
                     PPDFPDDDLAAAKVTNNNATGIIPPAGMNINQIYDPVNKRLFCNSNNKTITLRPGVYF
                     FTQVLLTGNSAKVQISPAGEAKIYIETVNPGDLAMHINNNGTLNPAGAPGNMEILIKQ
                     GRVWWENAGQIRCPVFAPNSDIDADNAFDLFGSIVCKTIDISGGGTFIYDEALGNPSP
                     AQTGIRVLAWLEITPLE"
BASE COUNT          919 a         1097 c         1130 g          957 t
ORIGIN      
        1 ctgacttgag attgggagtg tgaaactcac tccagccaca catgaaccaa agggcatagt
       61 ggcgatcggc aaagttcgag aagcaaactg gatcatagcc gtgcttcttg agttgcctcg
      121 ggaacatctg attggcgtcg tggggaccac catagtctcg cgtcgtgatc cgaaatcgag
      181 cgccagggcc gacattggaa accacgccgt ttcgcacgcc aaatcgccct gaagtcagac
      241 aggttctcga tgggacacag ggagaactcg cgcagtaata gtgatcgaaa caaatcccct
      301 cttttgcaat ggcgtcaatg gtcggcgacg tgggacggtt gtatccatag cagcccagat
      361 gatccggccg aagacagtct atgtcgaagt agatgatgcg cataacgtgc cccttcctat
      421 gatcttccac ccaagagtga gatgtagtcg ttcatcaatt tctcctgcgt ctcgaaggag
      481 tcaacgttct caaatgcttc aagggcagga tcattcgtcc tctgcatcca tcgctgcagt
      541 tctctccgta gtcgctggag atctttccga taacgtggaa caccaacgag gttgcggagc
      601 gcgtcagggt cattctggaa gtcgtagaac tcctctggaa cacgatggag aaacaactcc
      661 acgcgaccgg caattcgtgc atcgttctca cccgccgcaa gcatcgcttt catggtgcgt
      721 ccgtcctgag actcattgac aaagacacgg cttccgttcg accagggatt gaaaacgtag
      781 ccaaactgtc tgtcctgtac acaacgaatt ggatagttgc gtcgccccgc ggtctgatga
      841 aactgcgtga acacaagatt tcggttggcc tgcttctgtc cccggagtac aggcaagaat
      901 gatcttccgt ccatgctgtc aggaggatca atgccaacca tatcgagaag cgtcggcatc
      961 agatcaatgc cggagatgca atgctctgag tcaagtgaac cgggatgaat caccccttgc
     1021 catcgcatga tccatggtgt tcttgtgctg tgcagatagc agtttgtttt ggcgaacgga
     1081 aacgccatcc cattgtcaga caagaagaca acaacggtgt tctgctctct tcccgtctcc
     1141 cgaagcacgt ccagaattgc gccaacggta tcatccgctc ggcgtacgga gctgtagtat
     1201 tccgaaatct ccagtctgac ctctgggaga tcagcgagaa agcccggtgt tgttacggac
     1261 tgaggatcaa aggtgcgcga cggcacagcc gcgggcggcg tctgtcccgg ttggtaccat
     1321 tccgcgggat cattgccata gaaaggtctg tggggatcat gggagttcgc catcaggaag
     1381 aaaggctttc cgctcgcctg cgcctgatcc acaaaggcac gggcgtgctg acgatagaga
     1441 gccgggtttc tgcccttgcc cagtttcccc acgtcttggg tgtaatccca tttgaaatca
     1501 gcgtagggtg tcgaatgctc cacctttccc agaatgccca ctcgataacc ggcattccga
     1561 agcagctccg gcaagatggg aatacccgcc gttcgcaaat gaaagaaacc ttccccacca
     1621 cagcgatgag gatagcgacc agtcatcaac accgaacggc tcggctgaca gaccgctatg
     1681 gtgacatgtg catggttgaa tcgcactcct tcggatgcaa gacgatcaat gttcggagtc
     1741 gttccgctta ccggacagcc ataggctccg acggcatccc agttcatgtc atcggcagtg
     1801 atgagcagta tgtttggacg atcggaaatg atctcctcct tcaggcagaa ctttcctttt
     1861 cggcatcggg cgaagagaat gctagacgcc gaatggtgtc cctgtcaacg gagcgtcatc
     1921 tcctatcctt cggcagcaaa gcggagcatt gctaccgaga gatgtgaaag catacgtaag
     1981 agttcacgct tcagcgtgtt cctcttctcc ccgtaggagt tcacgcttta gcgtgtcctt
     2041 ttccggagca acctaaaggt tgaactctta cggctctttc cggagagcat gtgaacgtgg
     2101 tggcactttc tatcggacag cgtacccgac gtccctcttt gatcgcttgc tttggttccg
     2161 tctgcgttat atcttaaata gagaacggta tctgaatctt ttcaaactga gagtagttcc
     2221 atggacaaag tgaagttagg tgtcatcggt tgtggtgtta tatcggcaag ccatctggat
     2281 ctcgcaaaca agtgtccgat ggtagaggtc gtcgccgtgg cagatctgat tcccgaacgc
     2341 gcgcattcga gagcgcagac ttttggggtt cccaccgtct actacaacga tgaagatctg
     2401 ttgaatgatg agcgtatcga agccgtggtg ctcgcgatgc cggcgggcgt tcgaacgccg
     2461 gttgccttca aggcgctgga gcgcggcaaa cacgtgctgc tggaaaaacc cgtggcgagc
     2521 aatgtccatg aagtggagaa gatgattgcg atgcgtcgcg accgcgtcgt cggctgttgt
     2581 tcctctcgga tgactttttc cggtcatgct caaacggctg cagcatgtgt tcgttctggt
     2641 gtcctgggga agattcgcct gctgcgcatc agggcggtac atcccgccgg cgctcaaccg
     2701 aatccagagc cgccaccgtg gcgtgagagc atgcagctca atggaggagg gattctggtc
     2761 aactggagtt gttacgaact ggactatctg atgcacatcg tcggctggca gatctgtccc
     2821 aaagcggtga tggcgcagtg gtggccggtt gccgagaaga tgagtgccta cgtcgccgcg
     2881 ggttctgatg ccgatgcaca ttacacggca atgatcctct gcaacgacgg cgtcgctttg
     2941 agcatggaac gcgcggagtt ttcaagtatc tccgcagact gcgcgtggga gattatcggc
     3001 accgacgctt ccctgcatct tccgatgcat atccgaaaag gcgttcccaa cagtgtcgtt
     3061 ctggatcggt ttgtgcctgg agagggaatt gtgtcagaaa cgctttggga accggggcag
     3121 ggtgatcctg acgagaacgt gattaccgat ttcgttcagg cgattcagga acaccgggaa
     3181 ccgagaacga acctggagcg tgctctcgtg ctgcagaaga taaccaacgg gatttacggc
     3241 tctgcggcat ccggttcatc cgtggctgtc tagcctgttg tggaactgct ctttgcaccg
     3301 gagcaacaaa cactcgttgc agacactgca caaaaacgac aggggcgacc aaccgggcac
     3361 tcctgtcatc cagacgactt tgagcaattc ctgctattcc agcggggtga tctccaacca
     3421 ggcaagcacc cgaattccag tctgtgccgg agacgggttg ccaagcgcct catcgtaaat
     3481 gaacgtgcct ccaccagaga tatctattgt cttacacacg atcgaaccaa acaggtcaaa
     3541 ggcgttgtcg gcatctatat cggaatttgg agcgaagaca gggcaccgaa tctgacctgc
     3601 gttttcccac cagactctgc cctgtttgat gaggatttcc atgttcccag gggcacccgc
     3661 aggattcaac gtcccgttgt tgttgatgtg catcgccaag tctcccgggt tgaccgtctc
     3721 gatgtatatc ttggcttcgc cggcaggaga tatctgaacc ttggcgctgt tacccgtcag
     3781 aaggacctgc gtgaagaaat agactccagg tctcagagtg atcgtcttgt tgttgctgtt
     3841 gcagaagagt ctcttgttca ccggatcata gatctggttg atgttcattc ctgcaggtgg
     3901 aatgataccc gtcgcattgt tgttggtgac cttggcagca gccaagtcat catcggggaa
     3961 gtctggcgga tcggcgatgt cgccacccgt tgtcatggta ttgggttgtc cgtcaacatt
     4021 aagaccattc actcttgcgc cacccgtcaa cgcacctacc gcctcgatac ttccgaaaac
     4081 atccgtaccg ttgtccatgt gga
//
LOCUS       DAOQUC010000170         4096 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_24511_length_4096_cov_3.590695, whole genome shotgun sequence.
ACCESSION   DAOQUC010000170 DAOQUC010000000
VERSION     DAOQUC010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4096
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24511_length_4096_cov_3.590695"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(89..970)
                     /locus_tag="PKH07_04180"
     CDS             complement(89..970)
                     /locus_tag="PKH07_04180"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNT34176.1"
                     /translation="MFIDIHVHTRRIPGFPRVGTGKTYATPQQLLERYDALGIESAVL
                     LPGVSPEHAHVPQSIEEVIELSHEYPGRFIPFCNIDPRMLKNTADAPLGNIIAFYKDK
                     GCKGVGEVTANLPFDHPFVLNLFHHCQQQQMPLTFHIGHAIGGCYGLYDEPGLPLLER
                     ALQKFPDLIFLGHSQPFWAEIAPLETEQARKGYPKGRVEKPGRVVELMQRYPNLHGDL
                     SAGSGHNAVSRDEEFGCWFLNEFQDRLYFGTDICAPDTPTPLVGYLLQLRDEKKISET
                     CFLKIARENAVRLLNLQ"
     gene            complement(1029..2297)
                     /locus_tag="PKH07_04185"
     CDS             complement(1029..2297)
                     /locus_tag="PKH07_04185"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: protein motif:HMM:NF013413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HNT34177.1"
                     /translation="MEPTFFPITGYKPVLISFPNHASAIVLPMSQKRMPACGKAGGED
                     DANEMNTASALHQDALVFDAHSDTLLRVADKNYQLADRHTEGDMDLPRMREGGLDVAG
                     LAIFVHPRKHEGRIRERTLQLFQALERQLDLNSDLMTLARTTAEAETIVAKGKIAIFV
                     GLEAGDAIQDSLDLLREYYERGVRYMTLTWINSNNWAGSSGESEKKQGMTDFGFKVVE
                     EMNRLGMMVDLSHASDETVRDALRVSAQPMILSHSCSRSLCNIPRNIPDDLAQAIAER
                     GGVVGVNYCLDYLDASFGERIKALRDQFAPREKELREHYKDEPEVLQQHLEALRTERK
                     QAAACFLAARSDYRIVVDHIDHFVRLLGIDHVGLGSDFDGVEYFPSGLEDVTKLPCLT
                     EEMQARGYDEVAIRKILGGNFMRVFRKVIG"
     gene            complement(2448..3356)
                     /locus_tag="PKH07_04190"
     CDS             complement(2448..3356)
                     /locus_tag="PKH07_04190"
                     /inference="COORDINATES: protein motif:HMM:NF024793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNT34178.1"
                     /translation="MKTSAEILGHFRLGKMPFADTVNARFFYQTEAAERAFLRMEHCV
                     ESGRALGLIWGPSGSGKTLLSQLLLENLDPAKYLPILVLATPRMSKTSVLQELLSELE
                     IQDCPRSAFSMINLVHQRILREQREGRRVIILLDEAHFLSTDALHLLRTLSNLETTDE
                     KLVTVILFAEPGFVKRIQLPTYESLRGRIAFRAKLSPLTVEETEQYVKYRLLMAGGDG
                     TLFATECYRILHDASGGIPRSINRIAEGALLEGFLAGTATIDSEVMLRAVARDGWDQE
                     TPPSSEPVERRKKTLLETGRVLQYGS"
     gene            complement(3356..>4096)
                     /locus_tag="PKH07_04195"
     CDS             complement(3356..>4096)
                     /locus_tag="PKH07_04195"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNT34179.1"
                     /translation="LSVNLAIELQKRGRNCILVDVDLGLANAHIVAGVAPPVTLIDVT
                     EGVYSLAEVIVEGPAGIGMIAGGSGVLELANLPGYKRTQLLMELEKIEHRVDYIILDM
                     GTGISATVLDFMAAADASLVVTTPDITAVTDAYGMLKVLNRYGVASRAGIICNQVSSA
                     TQGKEIFNKLQTCCSRFLDFSPHYWGHIFKDSSVGKATAERVPYVLSHPYSRASRCTS
                     LIAESIYREPISKKESSFRMMRSGTTGT"
BASE COUNT          932 a         1110 c         1072 g          982 t
ORIGIN      
        1 cgatgggtta taactcgcac agcatgaacg ctgattctgt ggagcgacta tccatagacg
       61 cgcgacgaga ctggggcaat gcagggatct actgcaggtt cagcagtctg accgcgttct
      121 cccgcgcgat tttgagaaaa cacgtctccg atatcttctt ctcatctcgc agttgcagga
      181 gatatccaac caacggtgtc ggtgtatccg gcgcgcagat atccgtgcca aaatagagac
      241 gatcctggaa ttcgttgaga aaccaacacc cgaattcttc gtcgcgactg actgcattat
      301 gcccgcttcc tgcagaaaga tcaccgtgga ggttgggata gcgctgcatc agttcgacga
      361 ctcttcccgg tttttccact cttcccttgg gatagccttt tcgcgcttgt tctgtttcca
      421 gcggagcaat ctcagcccag aacggctgag aatggccgag aaagataagg tctgggaatt
      481 tctgcagcgc cctttccaaa agaggcagac cgggttcgtc atacagaccg taacagccgc
      541 cgatggcatg cccaatatga aatgtgagtg gcatctgctg ctgttgacag tgatgaaaaa
      601 gattcagaac aaagggatgg tcgaacggta gattcgcggt gacctcgccc acgcctttgc
      661 agcccttgtc tttgtagaaa gcgataatgt ttcccagagg agcgtccgcg gtgttcttga
      721 gcatccgagg atcaatgttg cagaacggga tgaatcgccc aggatactcg tgactgagtt
      781 cgatcacttc ctcaatggac tgtgggacat gcgcatgctc cggactgact ccgggaagca
      841 gtacggctga ctcgattccc aaggcgtcgt agcgctccag caactgttgt ggcgtggcgt
      901 aggtctttcc cgttcccacc cgcggaaaac cagggattct tctggtatga acatgaatat
      961 ctatgaacat gcctgtggac tcctgtcaag acgttatgcg ttcgtcaacc tactttcgta
     1021 tcagtatatc atccaatgac ctttcgaaag actctcataa aattgccgcc caggattttc
     1081 cgaatggcca cttcgtcgta acctcgcgcc tgcatttcct ctgtcagaca aggtagtttc
     1141 gtcacatctt ccaacccgct tgggaagtat tcaacgccgt cgaaatcaga acccagtccg
     1201 acgtgatcaa tgccaagcag tcggacgaaa tgatcaatgt gatccactac gattcgataa
     1261 tccgagcgag ctgccagaaa acaagctgca gcctgctttc tctctgtacg aagtgcttcc
     1321 aggtgctgct gcaacacttc gggttcgtcc ttgtagtgct cccgcagttc cttctctctc
     1381 ggggcgaact gatcgcgtaa tgccttgatc ctttccccga aggaggcatc gaggtaatcg
     1441 aggcagtagt tgactccgac aacgccccct ctctctgcaa tcgcttgagc gagatcgtcg
     1501 ggtatgttac gcggtatgtt gcagagactg cgcgagcagg aatgagagag gatcatcggt
     1561 tgggcgctga ctcgaagtgc atcccgaacc gtctcatccg aggcgtgaga gagatccacc
     1621 atcatgccca gtcggttcat ctcctcgacc actttgaagc cgaaatctgt catgccctgt
     1681 ttcttctctg actcacccga ggaacctgcc cagttgttgg agttgatcca ggtcagcgtc
     1741 atgtaccgaa cgccgcgctc atagtactcc cgaagcaaat ccagactgtc ctgaattgca
     1801 tcccctgctt ccaaacccac aaagatggcg atctttcctt tggcaacaat tgtctccgct
     1861 tcggccgttg ttcgcgccag tgtcatcagg tcactgttga gatctaattg cctctcgagt
     1921 gcttgaaaca gttgcaaggt gcgttctcga atacgacctt catgctttct ggggtgaacg
     1981 aagatggcaa ggcctgccac atccaagccg ccttcccgca ttcggggaag atccatgtcg
     2041 ccttcggtgt ggcggtctgc cagttggtaa tttttgtcgg ccactcgaag caacgtgtcc
     2101 gagtgcgcat caaaaacaag ggcatcttga tggagcgctg aagcagtgtt catctcgttt
     2161 gcatcgtcct caccgcctgc cttgccgcag gcaggcatcc gtttctgtga cattggaaga
     2221 acaatagcag aagcgtggtt cggaaacgag atcagaacag gtttgtatcc tgtgatgggg
     2281 aaaaacgtgg gttccacagg atagaggcct cttcggggag taagtccttg gaagtttgcg
     2341 tcctgacacg actcttgatg atcggatgat ccgtcagttg gctctcgtgt gtgccgtttg
     2401 gaaatccagg gctctgtctg gattgcgagt gaaatcgcag ttgtgtctta ggacccatat
     2461 tgaagcaccc gtcctgtttc cagcaatgtt ttctttcttc tctcaacggg ttctgaactg
     2521 gggggtgttt cctgatccca tccatcccgt gccacggcac gtagcatcac ttccgaatca
     2581 atcgtggctg ttccagcgag aaatccttcc agcagcgccc cttcggcaat tcggttgatg
     2641 cttctgggaa taccgcccga ggcgtcatga agaatgcggt aacactccgt ggcaaacagt
     2701 gtgccatcac caccggccat cagaagtcgg tatttcacat actgctccgt ttcctcgaca
     2761 gtcaatggac tgagtttcgc ccgaaaggca atccgtccgc gaagggattc ataggtcggc
     2821 agctgaatcc gctttacgaa accaggctct gcgaagagga tgacagtcac tagtttttcg
     2881 tcagtggttt cgagattcga gagcgttcgg agaaggtgca gtgcgtctgt ggacaggaaa
     2941 tgcgcctcgt ccaagaggat gatcacacgg cgtccttccc gttgttccct taggatgcgt
     3001 tgatgaacca ggttgatcat cgagaatgca ctacggggac agtcctgaat ctccagctcg
     3061 gaaagcaatt cctgaagcac acttgtcttg ctcatccgag gagttgccaa tacaagaatc
     3121 ggaagatatt tcgccggatc gaggttctcc agcaaaagct gtgacagcag cgtctttccc
     3181 gagccgctcg gcccccagat cagacccaac gcccgaccac tctcaacaca gtgttccatg
     3241 cgaaggaagg cgcgttcggc ggcctcagtc tggtagaaaa atcgggcgtt gaccgtatcc
     3301 gcgaacggca tcttgcccag tcgaaagtgt cccagaattt ccgcggaagt cttcatcagg
     3361 ttcccgtcgt gccgctgcgc atcattcgga aggaggattc cttcttggaa atcggttcgc
     3421 ggtagatgct ctcagcaatc agactggtac accgcgacgc ccgactgtaa ggatgggaaa
     3481 ggacataggg cacgcgttct gcagttgcct ttccgaccga agagtctttg aaaatatgtc
     3541 cccaatagtg tggggaaaaa tccagaaatc gtgagcagca cgtttgcaac ttgttgaaaa
     3601 tctccttgcc ctgagtcgcg ctggaaacct ggttgcagat gattccggca cgagaggcaa
     3661 ctccgtagcg attcaagact ttcaacattc cataggcatc tgttactgcg gtaatatcgg
     3721 gggtagtcac cacaaggctg gcatccgccg cggccataaa atccagaaca gtggcggaga
     3781 ttccagttcc catatccagg atgatgtagt ccaccctatg ctcaattttt tcaagttcca
     3841 tgagcaactg agtccgcttg tagccaggaa gattggccag ttcgagcaca ccagatccgc
     3901 cggcaatcat gccgattccc gcgggacctt ccacgataac ttccgcgaga ctgtagaccc
     3961 cctcggtcac atcaatgagt gtgaccggtg gtgcgacgcc ggcaacaatg tgagcgttgg
     4021 caagtcccaa gtccacatcc accagaatgc agttgcgtcc acgtttttgg agttcgatgg
     4081 cgaggttgac cgaaag
//
LOCUS       DAOQUC010000171         4078 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_24662_length_4078_cov_3.130748, whole genome shotgun sequence.
ACCESSION   DAOQUC010000171 DAOQUC010000000
VERSION     DAOQUC010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4078
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24662_length_4078_cov_3.130748"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..707)
                     /locus_tag="PKH07_04200"
     CDS             complement(<1..707)
                     /locus_tag="PKH07_04200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34180.1"
                     /translation="MPEQELTLGDILGLLLKRWGVLVVSFVVAFVVVLSLTHVLPDMY
                     RGEATLLVMSSKLRNKQMGQDYLGLTVESFLSLVANKQALSNLLDEVVAYKNAHPETP
                     LPQKMTLRDISRRVRVAEVRNSQCISIKVDMEVPELASDLANRLADRAVVANSELLGM
                     DSLDSVHHIQTRVTSSSDELQLAMDALLEFKKRARTEELDMAVRIAFGQIEAWEVEKQ
                     NLEVRTLQSGRRQFARP"
     gene            complement(707..1918)
                     /locus_tag="PKH07_04205"
     CDS             complement(707..1918)
                     /locus_tag="PKH07_04205"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNT34181.1"
                     /translation="MKKNSGDRFLCVCLLFALVLAGCSPKAEQSSDLSQSKNESEPVK
                     TVPTVLDDDPIVAEFGEKGIIRFSALLTFTKNKEDNVMPIRAEDTRVSLIKELRLKTL
                     QQLMDSELIYLEAEKQGISLPEESLNNMIQEMIQKNYGGSEEKLLQTLTEKGMDMAKL
                     QEALRKRFMTDELVRRTVSENVRVDEAELEPYYQQHLKEFTRPESSDVHFLVIKASSP
                     EGKQEARAKIESLRSEIVALLENEQDMTAKVERVVAFCREHSQDENRQGGSWWKVFGR
                     GGHIDRGLEEAASEAPLGQFGSIFELEKAPGYVTAFKKEYSPEETQPFEDAKAQIERI
                     LRKERRDTAYADLQQRVRTSGNIRIYEDVLYSRIPEGDRLETSSDPMAQEETSGSHGG
                     DHDPFQGGYAR"
     gene            complement(1941..2879)
                     /locus_tag="PKH07_04210"
     CDS             complement(1941..2879)
                     /locus_tag="PKH07_04210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34182.1"
                     /translation="MNRVLLAVLVALVCIVGLVLILVQSSDRLEMTPITVSPSISLRE
                     EQPGDIMALAQEDKKDFLESLRSQPAGTEHSEPSDIYEATDEPDYSALLRAKTAQWIY
                     RTFSQIGDTKIATMENNETGHQWEVHEKEVYDDVIIDDLAYNRCIVRLEDATAEIPFS
                     PKLTINIDDIFSRPPTEEEIAERVAYYDRHVRPTFEAMAKNYTPMPHQTMPVPLSPEQ
                     QATNVEHYMQNIAPTFIERAKSYTPKPGEKMPVRPASTQEVEINVKKYLATYHPTQLA
                     TMYPEWRKYFEDREVWDRNYGGTPVPSPNQAPPSNE"
     gene            complement(2997..3953)
                     /locus_tag="PKH07_04215"
     CDS             complement(2997..3953)
                     /locus_tag="PKH07_04215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34183.1"
                     /translation="MSQTVVVVVCVVVLVGIVGVSLFLRAHNERHYDLSEIKLQPAIS
                     LRENPPETIAVELSDSEKLLEDFLPLVREKAASVATESSPEIVIEKTDRDILLEKIAK
                     WNYRSFMQVGDQKSGTFENDETSQSLQVTEGYEFDGVEFVRLTENMAVVALGSASETL
                     SLVLKQEPPPVEEKATSGPPSKEERDERRRRYQEQIMPTFQHIAARYTPLPWVTIRTT
                     PPSPEEVQTAVAAYYETMRPTIEASTKIPGHPPSDEPLSEEEESRRAEWVATQIAVMR
                     ERHAEPQDGSSGESDNRRRGRPVPAPNQTPQEMLEPTDDLQD"
BASE COUNT          891 a         1163 c         1002 g         1022 t
ORIGIN      
        1 catggccgtg cgaattgcct tcggccagat tgaagcgtgc gaacttcgag attctgcttc
       61 tccacttccc acgcttcaat ctggccgaag gcaattcgca cggccatgtc gagttcttcc
      121 gtacgtgccc ttttcttgaa ctccaggaga gcatccattg ccagttgcag ttcgtcagac
      181 gaactggtca cgcgagtctg aatatgatgc accgaatcca acgaatccat ccccaagagt
      241 tccgaattcg ccacgacagc ccgatccgcc agacggtttg caagatcact ggcgagttcc
      301 ggcacttcca tgtccacttt gatactgatg cattggctgt tgcggacttc tgcaacacgg
      361 actcttcttg agatgtcccg aagtgtcatc ttctgcggca gcggagtttc cggatgcgcg
      421 ttcttgtagg cgacaacttc atccaacaaa ttcgacagtg cctgtttgtt tgcgaccaga
      481 ctgagaaaac tctccacagt gagtccgaga tagtcttgcc ccatctgctt gttgcgaagc
      541 tttgaactca tgaccagaag agtcgcttca ccacggtaca tgtctggcag tacatgcgtc
      601 agggagagaa cgacaacaaa agcgacaaca aaggacacca ccagaacgcc ccatctcttc
      661 agcaagagac caagaatatc ccccagcgtc agttcttgtt ccggcattat cgtgcgtagc
      721 ctccttgaaa aggatcatga tctcccccat gggagcctga ggtctcttct tgcgccatgg
      781 gatcactgga ggtttccagc ctgtccccct caggaattct tgagtaaagc acgtcttcat
      841 agatcctgat gttgcccgat gtcctcacgc gctgctgcag gtcggcgtag gcagtatctc
      901 gacgctcttt ccgtaggatt ctctcaatct gagccttggc atcttcaaac ggttgagtct
      961 cttcaggcga gtactccttc ttgaaggcgg tcacatagcc tggcgctttc tcaagttcaa
     1021 agatcgaacc gaactgaccc aatggcgctt ctgaagcagc ctcctccaga ccgcggtcaa
     1081 tgtggcctcc ccgcccaaag actttccacc agctgcctcc ctgacgattc tcatcctggg
     1141 aatgctctcg acagaatgcc accactcgct cgactttcgc cgtcatatcc tgctcgttct
     1201 caagcaatgc cacaatctcg gaacgcaatg attcgatctt cgctctggcc tcctgcttgc
     1261 cttccggaga cgacgccttg atgacaagga aatgaacatc ggaagattcc ggcctcgtga
     1321 actccttcag gtgttgctga tagtaaggtt ccagttcggc ttcatccaca cgaacattct
     1381 cgctcacggt tcgcctgacc agctcatcgg tcatgaaccg ctttcgaagc gcctcctgga
     1441 gcttggccat gtccatgcct ttctcggtaa gagtctgaag cagtttttcc tcggagcctc
     1501 catagttctt ttggatcatc tcctggatca tgttgttcag actttcttcg ggaagggaga
     1561 tcccctgctt ctcagcttcc agatagatca gttcgctgtc catcagctgt tgaagggtct
     1621 tcaatcgaag ttccttgatc aacgacaccc tcgtgtcctc agcacgaatc ggcatcacgt
     1681 tgtcttcctt gttcttggtg aaagtcaaca gggcactgaa gcgaatgata cctttttcgc
     1741 cgaactccgc gacaatgggg tcatcgtcca aaaccgttgg caccgtcttg acaggctcgg
     1801 attcattctt actctgggaa aggtcggatg actgctcggc cttcggagaa caaccagcca
     1861 gaaccagggc aaaaagcaag caaacgcaca agaaacgatc tccgctgttc ttcttcacgt
     1921 aatcaactcc cgatgttcta ttactcgttg gaaggaggcg cttggttggg agatggaaca
     1981 ggagtccccc cgtagtttct atcccatacc tctctatctt caaagtactt cctccattca
     2041 ggatacatgg tcgccaactg agttgggtga taggttgcca aatacttctt gacgttgatc
     2101 tccacttcct gggtactggc aggcctgacg ggcatcttct cacccggttt tggtgtatag
     2161 gacttggcgc gctcaatgaa agtgggagcg atattctgca tataatgctc gacgttggtc
     2221 gcttgctgct ctggagaaag aggcacgggc atcgtctggt gaggcatggg agtgtagttc
     2281 tttgccatcg cctcaaaggt cggcctgaca tgcctgtcgt agtacgccac cctctcggca
     2341 atctcctctt cggttggggg gcggctgaaa atgtcatcta tgttgatggt cagtttcggg
     2401 gagaatggga tttccgcggt ggcgtcttcc aatctcacga tacagcggtt gtaggccaga
     2461 tcgtcaatga taacatcatc atagacttcc ttttcatgca cctcccactg atgccctgtt
     2521 tcgttgtttt ccatggtggc tatcttcgtg tcaccgatct gactgaaggt tcggtaaatc
     2581 cattgcgccg tcttcgctct cagcagggct gaatagtccg gttcatccgt cgcttcgtag
     2641 atgtcagagg gttcagagtg ttccgtgccc gctggctgag atcgaagaga ctccaggaaa
     2701 tccttcttgt cctcttgcgc gagcgccata atatctcccg gttgttcctc tcgaagtgag
     2761 atagacggtg agacagtgat cggtgtcatc tccagccgat ctgaggattg cacaagtatc
     2821 aacaccaagc ccacgatgca gacgagggca acgagaactg ccaacaagac ccgattcatc
     2881 tctcaaatct ccttgattct caatccttca tacgaacgcg aacatcagaa agaacgcttc
     2941 ccttctccga acgcctcaca cgagtggctg agggacaggt tccagtgctc gacacatcag
     3001 tcttgcaaat catcggtcgg ctcaagcatt tcctgcggtg tctgatttgg agccggcacc
     3061 ggacgcccgc gacggcgatt gtcagactca ccggaagacc catcctgagg ctctgcgtgt
     3121 cgctcccgca tgacggcaat ctgtgttgca acccactcgg cgcgccgtga ttcctcttcc
     3181 tcactgagcg gctcatccga aggaggatga ccaggaatct tggtgctggc ttcgatggtc
     3241 ggcctcatgg tttcgtagta ggcggcaacc gcagtctgaa cctcttcagg agaaggaggc
     3301 gttgtccgta tagtcaccca gggaagcggc gtataccgcg cggcgatgtg ctgaaaagtc
     3361 ggcatgatct gttcctggta ccttcgacgt cgctcgtcgc gctcctcttt gctgggtggc
     3421 cctgaagtcg ccttctcctc gacaggagga ggctcctgct tgagcaccag ggacagcgtc
     3481 tccgatgcac tccccagagc gacaactgcc atattctccg tcaaccgcac aaattcgact
     3541 ccatcaaact catagccctc tgtcacttgg agagactgag aagtttcgtc attttcaaaa
     3601 gtccccgact tctgatcccc cacctgcatg aacgagcgat agttccattt ggcgattttt
     3661 tccaaaagga tgtctcgatc tgtcttctca atgacgattt ccggtgacga ttcggtggca
     3721 accgaagcgg ccttctcccg aaccaagggt agaaaatctt ccaacaactt ctcgctgtcc
     3781 gaaagctcca cggcaatggt ctcaggggga ttttcccgaa gactgatggc tggctggagt
     3841 ttgatctccg acaagtcgta gtgtcgttcg ttgtgggcac gaagaaaaag gctcaccccg
     3901 acgatgccga ccaagaccac aacacagaca acgactacca cggtttgact catcggtaaa
     3961 acctctgtct ttgtgtgaac agatctgatt tcataggcag tatgtatatc acgtcctgca
     4021 ctgctcttca atgagacgca tcagaagcgc tcggaattcg aagagagccc gaaaatcg
//
LOCUS       DAOQUC010000172         4076 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_24683_length_4076_cov_2.964685, whole genome shotgun sequence.
ACCESSION   DAOQUC010000172 DAOQUC010000000
VERSION     DAOQUC010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4076
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24683_length_4076_cov_2.964685"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1363)
                     /locus_tag="PKH07_04220"
     CDS             complement(<1..1363)
                     /locus_tag="PKH07_04220"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside triphosphate reductase"
                     /protein_id="HNT34184.1"
                     /translation="MSTARIRFIRKRDGRLAPFQPEQITEAVFKAVKAVGGNDREKAE
                     KTSRQVVSILEVAYRGERIPTVEEVQDLVERMLIENGYSDVAKSYILYRDQRAKLRDA
                     SAVLRDAVNMVDDYLGRQDWRVNENSNMDYSLQGLNNYVSTSITAIYWMNSIYPAEIR
                     QAHMSGDFHIHDTGQLSVYCCGWDLMDLLLRGFGGVAGKVESSPSKHLRTALGQIVNF
                     FYTLQGEAAGAQAFANFDTLLAPFVRYDGLNYADVKQAIQEFVFNLNIPTRVGFQTPF
                     TNLTMDLVPSETLADQNVIIGGEPKPEKYGEFQQEMNMINRAFCEVMMAGDAKGRIFT
                     FPIPTYNLSKDFNWDDEELELLWRLTSKYGTPYFANFINSDMKPSDARSMCCRLRLDN
                     RELHKRGGGLFGSNPLTGSIGVVTLNMPRIGYQTAGSEDDFLNRVGILMDLARNSLEI
                     KRKV"
     gene            complement(1702..2466)
                     /gene="recO"
                     /locus_tag="PKH07_04225"
     CDS             complement(1702..2466)
                     /gene="recO"
                     /locus_tag="PKH07_04225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HNT34185.1"
                     /translation="MPYLNDEAVVLNSLRSREKDKVTVLMTRNHGLVRAVARRAFSPT
                     SRLGTALEMLSTLKVQLFRRSESQELFTLCQAVLIERSPLLEADYETLSCGSFLAEVI
                     SVVATAEHDESELFRILAQAQVALAQGVERQLLAVAFFASVTAIAGIAPQVGACCRCA
                     REISEECGEWFFLPVSGNFCCAACVLAGESAMRLSAETLRWLEHALQAPFQDCLRYPH
                     HSGCLLESAKVLSEQVQSHLGVKFRTLPYIVEMHNS"
     gene            complement(2447..3229)
                     /locus_tag="PKH07_04230"
     CDS             complement(2447..3229)
                     /locus_tag="PKH07_04230"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNT34186.1"
                     /translation="METDALIRLLREEIYEAKRFEVLGRVASQMAHDFNNLVQAVISH
                     AQVLELEGIAETEKRDSATELRRAAEQAGMLAGYLLNFRQIDRPEMTEIDLNALTSEG
                     LDLFQRSIGKNVVLKCALSGSPCRVMADRRHLERVLFQILSNASDATSGRGTISVTTA
                     VRDLDATPDEGCLCVQPGPYAVLSVQDNGSGMSDEIRERVFEPFFTTKEPSPGKGLGL
                     CFVKAIVTRHRGHIHIESRSGTLVQIFLPLLEGEATGALPER"
     gene            complement(3551..3880)
                     /locus_tag="PKH07_04235"
     CDS             complement(3551..3880)
                     /locus_tag="PKH07_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005993614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HNT34187.1"
                     /translation="MSKRIYVGNLAFSTTESELRELFEQFGQVDSVNVVSDRQTGRSR
                     GFAFVEMESEAADASIRELDGKDFGGRNLKVNEAKPREDDRRGGGGGGGRRFGGGGGG
                     GARRNRF"
BASE COUNT         1006 a         1175 c          959 g          936 t
ORIGIN      
        1 ggactttgcg tttgatctcc agactgttgc gggcaagatc catcagaatg cccactcggt
       61 tcagaaaatc atcctcagaa ccggcagtct gatacccaat cctcggcatg ttcaacgtca
      121 ccacgccgat gcttcctgtc agtggattgg agccgaacag tcctccccca cgcttgtgta
      181 gttccctgtt gtccagacgc agccgacaac acatgcttcg agcatccgaa ggcttcatat
      241 cgctgttgat gaagttcgca aaatacggcg tcccgtactt ggaagtcaga cgccacaaaa
      301 gttccaactc ctcatcatcc cagttgaaat ccttgctaag gttgtatgta ggaatcggga
      361 acgtgaaaat tcgacccttg gcatctccag ccatcatgac ctcgcagaag gcgcggttga
      421 tcatgttcat ttcctgctgg aactcaccat atttctcagg tttgggttct cctccaatga
      481 tgacattctg atccgccaga gtttcagaag ggactagatc catcgtcagg ttggtgaacg
      541 gtgtctggaa accaactcgc gtgggtatgt tcagattgaa gacaaactcc tggatcgcct
      601 gtttgacatc cgcatagttc agtccgtcat aacgcacaaa gggagccaga agggtgtcga
      661 aattggcaaa agcctgcgcc cctgccgcct ctccctgaag cgtatagaag aagttgacaa
      721 tctgcccaag cgccgttctc agatgtttcg aaggcgaact ctcgaccttc cccgccacac
      781 cgccaaatcc acgcagcagg agatccatca aatcccaacc gcagcaatag acgctcaact
      841 gcccggtatc gtgaatatga aaatccccgg acatgtgtgc ctgtcgaatc tccgccgggt
      901 agatgctgtt catccagtaa atcgcggtaa tgctggtgga aacgtagttg ttcagcccct
      961 ggagcgagta gtccatgttg ctgttttcgt tgactcgcca gtcctgccgc ccgagatagt
     1021 catccaccat attgacggca tcccgcagca ccgcagaagc gtctcgaagc ttggctctct
     1081 ggtcgcgata gagaatgtaa gacttggcca cgtcggaata cccattctca atgagcatcc
     1141 gctcgaccag atcctgaacc tcctccaccg tgggaattcg ctctccacga taggcgactt
     1201 ccaaaatcga aacaacttgt cgagaggtct tttcggcctt ctcgcgatcg ttcccaccta
     1261 cggctttgac cgccttgaac acggcttccg ttatctgttc gggctgaaat ggggcaagcc
     1321 gtccgtcacg tttcctgata aaccggattc ttgcagtaga cattactcgc tatctccaac
     1381 gatctagcat gtcatagaca ttctcttcac agccaaaggc aaatttgaaa tccaccacac
     1441 ttttccgccc ccttacttag tcatcgaatc aaggcgttcc gcctctcgat ttcacaagtc
     1501 cgtagaatag aaacctgaag tattttgtca agagaaaaat aacaagatat tgtgcattca
     1561 atggcttatg atcgtatata ttgcgctttg tcaaggggtt agagcaataa gttttttgcg
     1621 aattttttat tgaaaactca taaaacgctt tagccctttt tgagcgccaa atggctgttt
     1681 tgaacctaac tttttgtgct tttaggagtt atgcatctcg acaatatagg gcaatgtgcg
     1741 gaatttcacg ccaagatgcg attgaacctg ttctgaaagg acttttgccg actccagaag
     1801 acaaccggaa tggtgcggat accggagaca atcctggaaa ggcgcttgta gagcgtgttc
     1861 cagccagcgt agcgtttcag cgctgaggcg catggcggac tctcccgcca acacgcacgc
     1921 cgcacagcag aagttcccag aaacggggag aaagaaccat tccccgcact cttcggatat
     1981 ctccctggca cagcggcagc aggctcccac ctgcggagca attcctgcga tggcggtcac
     2041 tgaggcgaag aacgccactg ccagaagttg cctctccacg ccttgagcaa gcgcgacctg
     2101 cgcctgggcg aggatcctaa aaagctcaga ttcatcgtgc tcggcagtgg ccacgacgga
     2161 gataacttcc gccagaaaac tcccacagct cagcgtctca tagtccgctt ccagcagggg
     2221 gcttcgttcg atcagcaccg cctgacatag ggtgaaaagt tcttgcgact cggaacgccg
     2281 aaagagttga accttgagcg tggagagcat ttccaaagcg gtgccaagtc gggaagttgg
     2341 gctgaaggca cgccgagcca cagcccgcac cagtccatgg ttgcgagtca tgaggacagt
     2401 gaccttatcc ttctcacgag aacgaaggct gttcaggaca accgcttcat cgttcaggta
     2461 gggcacccgt cgcttctcct tccaaaaggg gcaaaaaaat ctgaaccaag gtacccgacc
     2521 ggctctcgat gtggatatgt cctctgtgcc gtgtcacaat agccttcaca aaacagagtc
     2581 ccagaccttt tccaggcgaa ggttccttgg ttgtaaaaaa tggttcgaag actcgttcgc
     2641 gaatttcgtc cgacattcct gaaccattgt cttgaaccga caatacggca tacgggcccg
     2701 gctgcacgca caggcagcct tcgtcaggcg tcgcgtccaa gtctcgaaca gccgtcgtga
     2761 cagaaatcgt tcctctgccc gaagtggcat cgctcgcgtt tgaaagaatc tgaaagagca
     2821 cacgctcgag atgtcttcga tccgccatga cccgacacgg acttccggac agagcacact
     2881 tgagcaccac attcttgccg atgctccttt gaaagaggtc gagcccttca gaagtgagcg
     2941 cgttgaggtc aatctcggtc atctcgggac gatcgatctg acggaaattg agcagatatc
     3001 cagccagcat tccagcctgc tcggctgccc gtctgagttc ggtcgcacta tctcgcttct
     3061 ccgtctcagc aataccctcc agttcaagca cttgagcgtg actgatgacc gcttgaacca
     3121 gattattgaa gtcgtgtgcc atttgggaag caacacgccc caagacttca aagcgctttg
     3181 cctcgtaaat ctcttcccga agtaagcgta tgagagcgtc agtttccatg ctgccacctc
     3241 tgtgctgatg acgtctgaga tctcagattc gaaaccccgg tccgtcattc gcatcatatc
     3301 agataatcaa ctctgagatt gagatcatcc tgttccagta gccgaaccat ccaggccaag
     3361 cgtggcgacg caacaaccac agagactctc tcgtcgaaga attaaatcgc tgttcagcac
     3421 cagaggccaa gttcagacca aagaaacagc ccgcctttcg ctcgaaagac gggctgatgc
     3481 ttttgtcatt cttctgtcac ccgaatgcga cctggtaaag gaacgccccg ggagaatgga
     3541 gaacgtccaa tcagaaacgg ttccgacggg cgccaccacc gccaccgcca ccgaaacgtc
     3601 tgccaccgcc accgccacca ccgcgacggt catcttcacg aggcttggct tcgttgacct
     3661 tcagatttcg tccaccgaaa tccttgccat ccagttcccg gatgctggcg tctgcggctt
     3721 ctgactccat ctcgacaaac gcaaacccac gtgaacgacc ggtctgtctg tcagaaacga
     3781 cattcaccga gtccacctga ccgaactgtt caaacaactc cctcaattct gactcggttg
     3841 tgctgaacgc cagattccct acatagatcc tcttcgacat aactactttc tcctgaaaaa
     3901 aatgtggcct caaatttgag ggcctctgct gcccttctct ctagaacaga aggcagcaaa
     3961 ggccagttgc tccagaaata gttcacacag gttgaaatca ctatgtttca acggtattca
     4021 atatacacac tcccccgaga gagtcaagaa aaatctcgcc gatacagatt tgcggt
//
LOCUS       DAOQUC010000173         4050 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_24916_length_4050_cov_3.929662, whole genome shotgun sequence.
ACCESSION   DAOQUC010000173 DAOQUC010000000
VERSION     DAOQUC010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4050
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24916_length_4050_cov_3.929662"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="PKH07_04240"
     CDS             complement(<1..314)
                     /locus_tag="PKH07_04240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34188.1"
                     /translation="MEEVDIFEIICGVIWIVGIAIGCLLCRAQTASANPEENLRALQE
                     IRQRYSDFLTLSRAPIEDKRLRRKVAQFDDDVQSLLDRQILDESDPLYGTFGDVTDER
                     SH"
     gene            729..1658
                     /locus_tag="PKH07_04245"
     CDS             729..1658
                     /locus_tag="PKH07_04245"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HNT34189.1"
                     /translation="MSEPQNKQMSLADITVGLVVSMRPKQWIKNLFVFASLAFSQNLT
                     HWHLLSETVVTFFLFVAVSSAVYLLNDVLDLESDRVHPQKCRRPIASGTVPKIWALTS
                     SGILAVGGIGICLALESVGYLGRGITLVLFIYLLINAAYSRWLKHVVILDVLLVSVGF
                     VLRAVGGGIAIKVQISGWLVLCTILVSLFLALSKRRHELVMLEGDAANHRQILKEYTP
                     YFLDQMIAVVTASTLMAYALYTMSPEATGRVGQRMIFTIPFVLYGIFRYLYLVHQHQK
                     GGDPTEIMLSDRPMIINVLLWVVAVTLILTEVL"
     gene            complement(1701..2699)
                     /locus_tag="PKH07_04250"
     CDS             complement(1701..2699)
                     /locus_tag="PKH07_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015890944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNT34190.1"
                     /translation="MGAVMEHSSLLRVSNLKKYFPIREGFLLHVKDSVRAVDDVSFDV
                     GCGATLGLAGESGCGKTTVGRCILRLLEATSGEVAFGDSPNLLSLSPKQMKPYRKHLQ
                     MIFQNPFASLNPRMTVGTTVAEPLKIFSLVSGSKARRDRVCELLEQVGLREEHMDQFP
                     HQFSGGQRQRIAIARALSMQPQLIICDEAVSALDVSIQAQILNLLKDLQQRLGLSYLF
                     ISHNLGVVQYMSDRVAIMYLGKIVEMAPRERIYANPQHPYTQTLMAAIPVPDPTVRRN
                     RQKANDEVPSSFSHPSGCRFHPRCPKAMPICSEQEPPLLEQGDTQVACHLYTRPMV"
     gene            complement(2772..>4050)
                     /locus_tag="PKH07_04255"
     CDS             complement(2772..>4050)
                     /locus_tag="PKH07_04255"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HNT34191.1"
                     /translation="ERAEQEGEPLASHLGFCADVLDRIRFDFRKTEEDVLAEIREFMP
                     DVTPQHLVQWREMNHLWFRFVDGQNWYFNRGVRNLLRLNAEVRKLRTISGKPTEFYVN
                     HIADVIKAASTSSTPYVLPTRLRISYQLTLKPGAVPPGEIVRCWLAYPIECNTQKDIK
                     LIESFPEVKHTAAPNYPQRTLYFEQPASASDEATVFKVVYEYTSLAQYQTVDPAKVVP
                     YDVNSELFKTYTASRDPHLVFTPEIVASAKEAVGDETNPYLMAKNIYDWVVTNITYTS
                     MLEYSTVPNLSAWSMKYRQGDCGVFGLLFIVMCRSVGVPARWQSGWSPEPGETDMHDW
                     AEFYVEPYGWLHADPSRGVIDSPDPLIRDFHFGNFDCYRLVCNSDYGRPLDPPKRFPR
                     SETVDFQRGEVEYSGNNLYYDKWSYSFEATVVK"
BASE COUNT          913 a         1109 c         1079 g          949 t
ORIGIN      
        1 atgtgggaac gctcgtcggt aacatctcca aaggtgccgt agagcggatc ggactcgtcg
       61 agaatctgcc gatccaaaag agactgaacg tcgtcatcga actgtgccac ctttctccgg
      121 agacgcttgt cctctatcgg agcacggctc agagtcagaa agtcagaata ccgctgccga
      181 atttcctgaa gtgctctcag attttcttcc ggattggctg aggcagtttg tgctcgacac
      241 aacaagcatc cgatggcgat tccaacaatc cagatcaccc cacagattat ttcaaagata
      301 tctacttctt ccaatgcaca ttctcctttg tctcatcact gatcatacgt ccaagagagt
      361 gagcatagcc cactcgcccg cgggtgccca cctgctcgga aaacggtttt gggaatcata
      421 cgcgccctgc cggtcagcgt aaagaaactg ttttgcgggg cgacgaaccg gaggcggctg
      481 cgtaccagat cagttacact ggcgatagag ccaggatatg cttcggagcg acgctaccga
      541 agcccgctgt ctggctggtc ccacttcggc agcgtcgcag agcctctgca accgaagcat
      601 acctctcccc cttccgtaac tgagccagta cgcggctgcg atggttttgc ttgagtcgct
      661 ttcgaaaagg gatatatgat atggttgtgt gcattgtgtc agaagcggtg ccgtgacagg
      721 aaggcagcat gagcgaacct cagaacaaac agatgagttt ggccgatatc actgttggcc
      781 tggtggtctc gatgcggcca aaacagtgga tcaagaatct gtttgtgttt gcctcgttag
      841 ccttttcgca aaatctcacg cattggcatc ttctgagcga gacggtcgtc acgttctttc
      901 tctttgttgc cgtttcctcc gcagtctatc tgctcaatga tgtgcttgat ctggaatcgg
      961 atcgagttca ccctcagaag tgtcgtcgtc ccatcgcctc gggaaccgtg cccaagatct
     1021 gggcgctcac gtcttcgggg atcctggcgg tcggaggcat cgggatctgt ctggcgctgg
     1081 aatctgtggg ctatctggga cgtggaatca cgctcgttct tttcatctat cttctcatca
     1141 acgcggcgta ttcaaggtgg ctcaaacatg ttgtgatcct tgacgtgctg ttggtatctg
     1201 ttggttttgt ccttcgcgcc gttggcggcg ggattgccat caaggttcag atctccggtt
     1261 ggctggtgct ctgtacgatt ctggtgtccc tttttctggc tctttccaaa agaaggcatg
     1321 aactggtcat gcttgagggt gatgcggcca atcatcgcca gattcttaaa gagtacacac
     1381 cttactttct cgatcagatg atcgctgtgg tgacggcatc aacgctgatg gcgtacgccc
     1441 tctatacgat gtcccctgaa gcgacgggac gtgtcggaca acgcatgatc tttaccatcc
     1501 cgttcgtgct ctacggcatt ttccgatacc tctacctggt tcatcaacat caaaagggcg
     1561 gcgatccgac agagattatg ctcagcgacc gtccgatgat catcaatgtg cttctctggg
     1621 tagtcgcggt cactcttatc ttgacggaag tcctctaacc cgatcccaag cgttcttgcc
     1681 gcgtcacgcc aatagtcctc ctagaccatc ggacgagtgt agagatgaca ggcgacttgg
     1741 gtgtctccct gttccagaag cggtggttcc tgttcggaac agatcggcat ggcttttgga
     1801 catctgggat gaaaccggca gcctgaggga tgagagaagg aactgggaac ctcgtcattc
     1861 gccttctgtc ggttccggcg caccgtcgga tcaggaacgg gaatcgcggc catgagggtt
     1921 tgagtatagg ggtgctgtgg attggcgtag atgcgctccc gaggtgccat ttcgacaatc
     1981 ttgccaaggt acatgatcgc cactcggtca gacatatact gaacgacacc gaggttgtgc
     2041 gagatgaaaa gataactcaa gcccagccgt tgctgcagat ccttcaatag gttcagtatc
     2101 tgcgcctgaa tggagacgtc gagcgcagac actgcttcat cgcagatgat cagttggggc
     2161 tgcatggaca acgcacgcgc gatggcgatg cgctgacgct gtcccccgct gaattgatgc
     2221 gggaactgat ccatgtgttc ctcgcgcaaa ccaacctgtt cgagcagctc gcaaacccga
     2281 tcccgcctcg ccttactgcc ggaaaccaaa ctgaatatct tcagtggctc cgcaacggtt
     2341 gtaccgactg tcattcgggg attcagtgag gcaaacggat tctgaaagat catctgaaga
     2401 tgctttcggt acggtttcat ctgcttgggc gacagagaca ggagattggg ggaatcgcca
     2461 aacgccactt cgcctgaagt ggcttcgagc agccgcaaga tgcagcgtcc aacagtggtt
     2521 ttcccacagc cgctttctcc agccaaccca agcgtcgcac cacatccgac atcgaaagac
     2581 acatcgtcca ccgcccggac gctgtccttg acgtgaagca gaaatccctc tctgatgggg
     2641 aaatacttct tgagattgct caccctcaga agggaagaat gctccatgac tgcacccata
     2701 ttgttgtgct ccgcgaaaac catcccgtag tgcgcaaact cagcgccaac attcagctat
     2761 tctggtcacc cttacttgac gacggttgcc tcaaaggaat atgaccactt gtcgtagtac
     2821 aggttgttgc ccgaatactc aacctctccc cgctggaaat ccaccgtttc tgatcgggga
     2881 aagcgcttgg gcggatccaa gggtcgccca tagtcgctgt tgcagaccag tcggtaacag
     2941 tcaaagttgc caaaatgaaa gtcccgaatc agaggatcag gagagtcaat cacaccgcga
     3001 gacggatcgg catggagcca accgtaaggc tcaacgtaga actctgccca gtcgtgcatg
     3061 tcggtctcac caggctccgg cgaccaaccg gactgccagc gtgccggaac acccaccgag
     3121 cgacacatga cgataaacag cagcccgaag acaccgcagt caccttgtcg gtatttcatg
     3181 ctccaggcac tgaggttggg gacggtggaa tactccagca tggaagtgta ggtgatgttg
     3241 gtcaccaccc aatcatagat attcttcgcc atcagatagg gattggtctc atcaccaacc
     3301 gcctctttcg ctgaggcaac gatctcaggc gtgaacacca gatggggatc acgggacgcg
     3361 gtgtatgtct taaacaactc gctgttgaca tcgtagggca ccaccttggc aggatcgacc
     3421 gtctggtact gcgccagaga agtgtactca tagaccacct tgaacaccgt cgcttcgtca
     3481 ctggcagaag caggttgctc aaaatacagc gttcgctgtg ggtagtttgg cgccgcggta
     3541 tgtttgacct cggggaaaga ttcgatcaac ttgatgtctt tttgcgtatt gcattcaatg
     3601 ggatatgcca gccagcaacg gacgatttcg cccggcggga cagcacctgg tttcaatgtc
     3661 aattggtagg agatccgaag ccgcgttgga agcacgtacg gcgtggacga tgtggaagca
     3721 gccttgatca catcggcgat atggttgacg tagaattcag tcggcttacc ggagattgtc
     3781 cggagcttcc tgacctccgc attgagccgg agcaggttcc gtacaccgcg gttgaaatac
     3841 cagttctgac catccacaaa acgaaaccag agatgattca tctctcgcca ctggacaaga
     3901 tgctgcggtg tcacatcagg catgaactcc cgaatctcag ccagaacgtc ttcttcggtc
     3961 ttcctgaagt caaaacgtat ccgatccagg acgtcggcac aaaagcccag atgactggcc
     4021 agaggctcac cttcctgctc ggctctttcc
//
LOCUS       DAOQUC010000174         4046 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_24956_length_4046_cov_3.569030, whole genome shotgun sequence.
ACCESSION   DAOQUC010000174 DAOQUC010000000
VERSION     DAOQUC010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4046
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24956_length_4046_cov_3.569030"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            43..1881
                     /locus_tag="PKH07_04260"
     CDS             43..1881
                     /locus_tag="PKH07_04260"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HNT34192.1"
                     /translation="MRTKGAAVGIQTMRTNLATLLFGVVLLTLTLVTPASARLSQPPM
                     IFYGYLQMPLGQPVTTGELLFLFIPPNGVTVRCKATLASFAAGVNFQLDVPVQKSPVT
                     IPETILTLDTSYQVEIYHNGFRVGAPELDSLMPAVPGRVVGPLLLTVSPALPVLASSG
                     GIDFGFAIFGEFTDRTYTVSNIGGQVLTGTVRLKTGIHYKLVESGIQVNSVSFSLDQG
                     KTRDITVRFHPAILDSRLSDVIQVRSNAGDVDDSLFGDSVNLTPTITPTRTPTRTPTM
                     TPTISEVLTDTPTTVPSLTATPSDLMTETPTAIGTETPTETPTETPTEEPTLTETPTD
                     TATAPDTETPTETPTTTGTETPTETSTEEPTLTETPTETPTITPTPIRTMMVKVQVAD
                     SSTGLPIAGASVYVLAPCAPGSQSGVTTSGGFLWFDGVAQGAIEVHAMAEGYGPGTVQ
                     PTLSVNGQTVFVNLVPLTGLFGSALVLVTLPDGTPFEGARITSDGVFAGTTNIYGSLL
                     LSNLNYSRLSHTVVVSAQGYFVPKASYEISSTQPYTVLRFVLIKVPTLNVDRLNKVDA
                     KDLLMLTQRLGTDDPLADFNGDGVVDWKDLFIFAGGWLGEGWLEQQ"
     gene            2020..2274
                     /locus_tag="PKH07_04265"
     CDS             2020..2274
                     /locus_tag="PKH07_04265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34193.1"
                     /translation="MSYRAWEDQTEGLVGSESNQNLYVLAEGHADTFGIAPVRGLAEN
                     ACRDLRRAFPAIFELQFDPNSTDFEVVFSRVRENKPVGEA"
     gene            complement(2329..>4046)
                     /locus_tag="PKH07_04270"
     CDS             complement(2329..>4046)
                     /locus_tag="PKH07_04270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34194.1"
                     /translation="VDEQGALLEISVVVVFGVYKCSQIVTRYKDGTITAVNGHPSERL
                     RTEAYGRSLDLPPNGYAGWTEDGKIDVRSSDLDGSRCDYAVTPSYIYVDGRGRFSRFE
                     KAAGDGIGICRILGQNRYEIILYDGAECGFAIPAVKAVALDKEGKEICPAELRLARKL
                     AYVLPVEGAFSYLVKGGRIRKDAEITSSKSWAVPGQRIRVTKATPLSLTPTSESNPLL
                     DEIQVPNDAKDGQRLWFRRDGSWIDFTCMPSAEVDIAIEETDLVVALKSNHEDERAVT
                     ISAMDRSQKMLASPQRTVQTRFPMETFSEETLEPLIVDVKGDGFDQRVEAILLLERKF
                     IPLLTLPIAWEKGMCLRGGQETGDFGTTQANVSFGQTACGGETKVGFAMHPPWVGGVG
                     YVFALYSPITFPCDHPAAFRAFVGKGDGSNAGDGILYRLGVVDESGHETIVAEQTVTK
                     HEWLPLEADMSPWMGKKVSLKLMADVGLNDDSSGDWACWADMRLESLDQTLAMTLERD
                     TDRFRMESGPYPLDHPDLKVLRTARSGRLHYEGQGLQGPGIYEINGHLNGIELGLLAP
                     ASGDE"
BASE COUNT          899 a         1155 c         1035 g          957 t
ORIGIN      
        1 agaccgaata cactacacac acagatgaag gagttttcag acatgagaac gaagggcgca
       61 gcagtcggaa tccagacgat gagaacaaat cttgccactc tgttgtttgg cgtggtcttg
      121 ttgacgctca ctcttgtcac gccggcatct gctcggctga gtcaaccacc catgatcttc
      181 tacgggtatt tgcagatgcc cttggggcag cctgtcacaa cgggtgaact gcttttcctg
      241 ttcatcccac caaatggtgt cactgttcga tgcaaggcca cgctcgctag cttcgcggcg
      301 ggtgtcaact ttcaacttga cgtgcctgtg cagaagtctc ccgtgaccat accggagacc
      361 atcctcacac tcgacacgag ctatcaggtt gagatctatc acaacggatt ccgggtcggc
      421 gctccggagt tggattctct catgccagcc gtgccggggc gggtggttgg tcccctccta
      481 ctgactgtgt ctcccgcctt gcccgttctt gcatccagtg gaggcattga ctttggattc
      541 gcgatttttg gcgagttcac cgatcggaca tacaccgtgt ccaatatcgg aggccaggtt
      601 cttactggca cggttcgcct gaagacagga atccattaca aactcgtcga gagtgggatc
      661 caggtcaatt ccgtttcctt ctcgctcgat caaggcaaga cccgcgacat caccgttcgt
      721 tttcatcctg ccatacttga ttctcgactt tctgatgtga ttcaagtgcg ctccaacgcg
      781 ggggatgttg acgatagtct gttcggtgat tccgtgaatc tcacgccgac aatcacccca
      841 actcgtactc ccacgagaac gccgacaatg acaccgacga tatctgaagt gcttacggac
      901 actccgacca cagtgccctc gctgacagca acaccttccg atttgatgac cgaaactccg
      961 accgcgattg gtacggagac accgacagaa acaccgacgg aaacaccgac agaagagcca
     1021 acactgactg aaacaccaac agacaccgcg accgcacccg atacagaaac accgacggag
     1081 accccgacca caactggtac tgaaacacca acggagactt cgacggaaga gccaacactg
     1141 actgagacgc cgacggaaac accgacgatc actccgacgc caatccgaac catgatggtc
     1201 aaggtgcaag tggcggacag ttcgacaggt cttcccatcg ctggggcttc ggtttatgtt
     1261 ctggctccgt gtgctcccgg cagtcaatct ggtgtcacaa cgtcaggcgg tttcttgtgg
     1321 ttcgacggtg ttgcgcaagg agctatcgaa gtccatgcaa tggctgaggg ctatggaccg
     1381 gggacggttc aaccaacgct ctctgtgaat ggccagacgg tatttgtgaa tctggtgcct
     1441 ctcacggggc ttttcggttc ggctcttgtc ttggtgactt tacctgacgg gacgcctttc
     1501 gaaggggctc ggatcacttc tgatggcgtc ttcgccggca cgaccaacat ctacggttcc
     1561 ttactgctgt caaatctgaa ctacagccgt ctttcgcata cggttgtagt gagtgctcaa
     1621 ggatactttg tacccaaggc ttcctacgag ataagcagca cgcaaccgta cacggtcttg
     1681 cgctttgtcc tgatcaaggt tccaactctc aacgtagacc gcttgaacaa ggtggacgcc
     1741 aaggatcttc ttatgctcac tcagaggctt ggcacagacg atcctcttgc ggacttcaac
     1801 ggcgatggtg tcgtggactg gaaggatctt ttcatctttg ccggtggctg gcttggagaa
     1861 ggctggcttg agcagcagta gtttgtccgg ttctgcgtgg acgaaatgac atggttgatt
     1921 gcgggcttct gcaagaggcc gggtgtgagg tgaattgagt ttaggctcaa gcgttgatcc
     1981 aaacgcccag gcagagtatt gctctcagac tgagctccct tgtcctatcg ggcatgggag
     2041 gatcagacgg aaggcttggt tgggtcagaa tcgaaccaga acctctatgt ccttgccgaa
     2101 ggtcacgcag acaccttcgg catagcgcca gtccgggggt tggctgaaaa tgcttgtcgc
     2161 gacttgagac gagcatttcc tgccattttc gagctccagt tcgatccaaa ctcgacggat
     2221 tttgaagtag tcttttctcg ggttcgagag aacaaaccgg ttggcgaggc gtagatcctc
     2281 aatcgccttt ctgggcaatg ggatggaagc gtgttcccat tcgttcccct actcatcgcc
     2341 agaagcggga gcaagcaggc ccagctcgat tccgttcagg tggccattga tctcatagat
     2401 cccaggcccc tgaagacctt gtccctcata gtgcagccgt cctgatcttg cagtccgcaa
     2461 aaccttcaga tcaggatgat ccaagggata cggcccactc tccattcgga agcgatccgt
     2521 gtccctttcg agtgtcatcg caagtgtctg atctagtgat tcgagccgca tatccgccca
     2581 acaggcccag tcgccactgg agtcatcatt gaggccaaca tccgccatca gcttcagact
     2641 caccttctta cccatccaag ggctcatatc tgcttcaaga ggcagccact cgtgctttgt
     2701 gacggtttgc tccgccacaa tggtctcgtg gcctgattca tctacgactc cgagtctgta
     2761 gagaataccg tctccagcat tacttccgtc acctttgcct acaaaagccc gaaatgccgc
     2821 ggggtgatcg cagggaaacg ttatcgggct gtaaagcgcg aagacgtaac cgactccacc
     2881 cacccatggc gggtgcatgg caaaacctac cttggtttca cccccacagg ctgtctgtcc
     2941 gaaggacacg ttcgcttggg ttgttccgaa atcgcctgtc tcttgaccgc cgcgtagaca
     3001 cattcctttc tcccatgcga tggggagcgt cagcaacggg atgaacttcc gctcaagcag
     3061 gagaatggct tcaactctct gatcgaagcc atcgcctttg acatcaacaa tcaaaggttc
     3121 gagggtctct tccgagaaag tctccattgg aaatcgagtt tggactgtcc gctgtggact
     3181 cgccagcatc ttttgacttc tgtccatcgc tgagatagtc acggctcgtt catcctcgtg
     3241 attgctcttc aatgccacaa ccagatctgt ctcctcaatg gcaatgtcca cttctgctga
     3301 gggcatacag gtaaagtcta tccaagagcc atctcttctg aaccaaagac gctggccatc
     3361 ctttgcgtcg tttggaactt ggatttcatc caaaagagga ttcgactcgc ttgtcggagt
     3421 taaggagagt ggggtggcct tggtgactcg gatacgttgg cctgggactg cccaagactt
     3481 ggaactggtg atttcggcat cctttcggat acgccctccc ttgacaaggt agctgaatgc
     3541 accttccacg ggaagcacat aggcaagttt tctcgccaga cggagttcag ctgggcagat
     3601 ctcctttcct tccttgtcaa gcgcgaccgc cttgaccgcc ggaatcgcga agccacactc
     3661 tgccccatca tagaggatga tctcgtaacg gttctgaccg aggattcgac agattccaat
     3721 cccatctccg gcggcctttt caaagcgaga gaaccgtcca cgaccgtcaa cataaatata
     3781 ggaaggcgtg acggcatagt cacaccgact gccatcgaga tccgaactcc gaacatctat
     3841 cttcccgtct tcagtccagc cagcgtaacc gtttggagga agatccaaac ttcgtccgta
     3901 tgcctctgtt cggagccttt cactcgggtg tccgttgaca gccgtgatcg taccgtcttt
     3961 atatcgcgtg acaatctgcg aacacttgta caccccgaag acaacgacta cagagatctc
     4021 caggagagcg ccttgttcat caacgt
//
LOCUS       DAOQUC010000175         4035 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_25055_length_4035_cov_3.346482, whole genome shotgun sequence.
ACCESSION   DAOQUC010000175 DAOQUC010000000
VERSION     DAOQUC010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4035
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25055_length_4035_cov_3.346482"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2275
                     /locus_tag="PKH07_04275"
     CDS             <1..2275
                     /locus_tag="PKH07_04275"
                     /inference="COORDINATES: protein motif:HMM:NF019556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HNT34195.1"
                     /translation="VGSVRARGWLLHQLEIMRDGMVGHLPELSRWCVEKDNAWARPDG
                     QGALGWEELPYWLKGYGDLGYLLEDQKIIEETRKWAEFALSSQEEDGYFGPRRNKQRY
                     DIWPNMVMLDVLRSYYDFSGDKRIIEFMKKYFQWQMSLPREYLLPGSWQKIRGGDNLY
                     SIHWLYNRTGEKWLLDLATAVHERTVNWTDGIKSWHGVNIAHGYREPAQYYQQSKDRK
                     HLDATERNYRAVIDEYGQFPGGMYATDENARPGYIDPRQGAETCSMVELMRSCEILLA
                     ITGDPIHADRCEDVAFNSFPASVMKDFMGLHYLTPANVVQLDRENKSPGFQNHGPMLP
                     FTPGEAFRCCQHNVSHGWPYYVEHLWMATRDNGLAAVLYAPCEVRAKVGKGTEVHIIQ
                     ETGYPFREQVQLQLHLPKPAKFPLYRRVPGWCNAASVLLNDEPLDVAIVSGSYVRVER
                     VWNDGDVIVLRLPMETKVRVWEKNKNSVSVVRGPLAYSLKIGESWKEFAREGRWGIWE
                     VFPTTPWNYGLVLDASGETYAANRLDVQVKDGEIPDQPFDIETAPIEIRARGCRIPLW
                     EMEQDGLVGRLQKSPIKTDEPEEEITLIPMGCAKLRITAFPVIGKGAEAREWRRIPPR
                     ISASFTQAYMAALDDGVEPMASDDTRMPPYVWGEASPDGYWVAYGFDEPRTVTKSRVY
                     WFDDKAQGQYRLPKSWRVLWKDGEQWREVTNASQYEIRMDQYSEVTFDPVTTEEIRLQ
                     VEPQDRVGAGILEWKVE"
     gene            2795..3190
                     /locus_tag="PKH07_04280"
     CDS             2795..3190
                     /locus_tag="PKH07_04280"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012494823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HNT34196.1"
                     /translation="MNADKLTDADIALLEAARRVIAQRFREGYHHVGAALRTKSGQVF
                     SAVHLEAYVGRIAVCAEAIALGMAAATGDTEIDTIVAVNRDGEVVAPCGMCRELITDY
                     APGCRIIIPGADGPTATSAGDLLPEKYRR"
     gene            3433..3564
                     /locus_tag="PKH07_04285"
                     /pseudo
     CDS             3433..3564
                     /locus_tag="PKH07_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882109.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            3820..>4035
                     /locus_tag="PKH07_04290"
     CDS             3820..>4035
                     /locus_tag="PKH07_04290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34197.1"
                     /translation="MKIVRNALAVIFAACVSAAYAADVTLEVTDLKDNVIVLQQARAY
                     QESSSTIPSKVVTFDGIEMELGSGRVVI"
BASE COUNT          978 a          973 c         1196 g          888 t
ORIGIN      
        1 cgtgggtagt gttcgtgcgc gcggttggct cctgcatcag ctggagatca tgcgtgacgg
       61 catggtcggc catctgcccg agctgagccg ctggtgtgtg gagaaggaca atgcctgggc
      121 acgccctgac ggtcagggtg ctttgggttg ggaggaactg ccctattggc tcaaagggta
      181 cggtgatctg ggttatttgc ttgaggatca gaagatcatt gaggaaacgc gcaagtgggc
      241 tgagttcgcc ctttccagtc aggaagagga cggctacttc ggccccagac gcaacaagca
      301 gcgctatgac atttggccaa acatggtcat gctcgatgtg ctgcgctcgt actatgattt
      361 ctctggtgac aagagaatca tcgagttcat gaagaagtac ttccaatggc agatgagtct
      421 gccgcgggag tatctgctcc ccgggagttg gcagaagatt cgcggtggtg acaatctata
      481 cagcatccac tggctctaca atcgaacggg agagaaatgg ctcctggact tggcgacagc
      541 ggtccatgag cgaacggtca actggacaga tggcatcaag agttggcacg gtgtgaacat
      601 cgcgcatggc taccgagaac cggcacagta ctatcagcag tccaaggatc gaaaacatct
      661 ggatgcgacc gaacgaaact accgagcggt gatagatgaa tacggtcagt tccccggtgg
      721 tatgtacgcc accgatgaga atgcgcgccc gggttatatt gatccacgcc agggtgcaga
      781 gacctgttcc atggtggagt tgatgcgttc ctgtgagatt ctactcgcaa tcacaggcga
      841 tccgattcat gccgatcgtt gcgaagatgt cgccttcaac tcgtttcctg cgtcggtcat
      901 gaaggacttc atgggacttc attatctgac gccggcgaat gtcgttcagt tggatcgcga
      961 gaacaaaagt ccgggctttc agaatcacgg cccgatgctg ccgtttactc cgggtgaagc
     1021 gtttcgatgc tgtcagcaca atgtctcaca tggatggcct tattatgtgg agcatctctg
     1081 gatggccact cgggataatg gtctggcggc ggtgctttac gcgccctgcg aagttcgagc
     1141 gaaagtcggg aagggcacag aggttcatat catccaagag actggctatc ccttccgtga
     1201 acaagtccag ttgcaactgc atcttcccaa gcccgcaaag ttccccctct atcggcgcgt
     1261 tcccggctgg tgtaatgcgg cgtccgtgct tctgaacgac gagcctttgg acgtggcaat
     1321 cgtttcggga agttatgttc gggtggaacg agtctggaac gatggcgacg tgatcgttct
     1381 cagactgccg atggagacca aggttcgcgt ctgggagaag aacaagaatt ctgtttctgt
     1441 cgttcgcggg cctctggcct attctctaaa aatcggggag agctggaagg aattcgcacg
     1501 tgagggacga tggggcatct gggaagtctt cccgacgact ccctggaact atggtttggt
     1561 gttggatgca agcggcgaga cctacgccgc gaaccgtctg gatgttcagg tgaaagatgg
     1621 ggagatcccc gatcagccct tcgacatcga gactgcgccc atcgagatcc gcgcccgtgg
     1681 ttgtcggatt cctttgtggg agatggaaca ggatggtctg gttggacgac ttcagaaaag
     1741 tccgatcaag acggatgaac ccgaagagga aatcacgctg attcccatgg gctgtgccaa
     1801 acttagaatc actgcctttc ctgtaatcgg caagggtgca gaagccagag aatggcgcag
     1861 aatcccacct cggatttcgg cgtccttcac gcaggcgtac atggcggctc tcgatgacgg
     1921 agttgaaccg atggcgtctg atgatacgcg aatgccacct tacgtctggg gtgaggcaag
     1981 tcccgacggt tactgggtcg cttacggctt cgacgaacct cgaacggtca cgaaatcacg
     2041 agtctattgg tttgatgaca aggcacaggg acaataccgc cttcccaagt cgtggcgtgt
     2101 gctttggaag gacggtgaac agtggcgcga agtcaccaat gcttcccagt acgagatcag
     2161 gatggatcag tactctgaag tcacttttga tcccgtaacc accgaagaaa ttcgactcca
     2221 ggtagagcct caagaccgtg ttggcgcagg cattctggaa tggaaggtgg agtgacctcg
     2281 cttctgagaa acatgacggc ggattctatg ggtgaaagag caccgaatag tcggccggga
     2341 ttcatcccgc gcagcatgaa tgctgcccta ctgggttgcc ctcgacccag ggtcccggtt
     2401 tcgggggaaa ccgggccagt tgctacctgc tgagccagta ccgagaagcg caagatttcc
     2461 ttgaagtcgt ttcgggaagg gatgtatact tcagacggag ttgtgggtcg ctaccgagaa
     2521 cagcctcatg gctattctgt ggaaagagca aagaaatcgt gcaggcaaca gtaacacagg
     2581 caatttctga gaatatctct gattcattat gagaggtctt tattggcatg atatatggaa
     2641 accatataag accccttaat attctataga acggatatat ttaattgata tatatgggct
     2701 taagaactta ctttatctag ggtcttatgc cggccggaaa tcagataaag gttcttatac
     2761 ggaaaccata tatacattgt tcggtcacaa gaacatgaat gcagacaaac tcacagatgc
     2821 cgacatagcg ctgcttgaag cagcccgtcg ggtcatagca cagcgctttc gcgagggata
     2881 tcatcacgtt ggcgcagcat tgcggacgaa gtctgggcag gtgttctcgg ccgtccatct
     2941 cgaagcgtac gttggtcgaa ttgcggtgtg cgccgaggca atcgctttag gcatggcggc
     3001 cgccactgga gatacggaga tagatacaat cgtcgcggtg aaccgcgacg gtgaagtagt
     3061 cgccccatgc ggaatgtgtc gtgaattgat tacggactac gccccgggtt gcagaatcat
     3121 catccctggg gcagacgggc cgaccgcgac cagtgctgga gacctgcttc cagagaaata
     3181 caggagatga ccgaacaacc gagacacgta gcctgggttg ccctcaaccc aggatcccgg
     3241 tttcgaggga aaccgggccg gttgctacct gctgagccag taccgagaag cgcaagattt
     3301 ccttgaagtc gtttcgggaa gggatgtata cttcagacgg agttgtgggt cgctaccgag
     3361 aacagcctca tggctattct gtggaaagag caaagaaatc gtgcaggcaa cagtaacaca
     3421 ggcaatttct gactcgaagg gggctatgac atccgcacga tccgggagtt gctgggccac
     3481 aaggacgtca gcacgaccat gatctacacg catgttttga acaaaggagg ccacggagtc
     3541 cgcagcccca tggacggtct atgaggatgg ccttatgccg atccgcataa cacgctgagc
     3601 taaatggcga gaccggcggg aacacattga gaaacaagaa gttgacgcat gtgaagtgcg
     3661 ggatacaaag gcgtcttata cggacagaaa cgatagtccc cgcgtcttat gcgggtcaat
     3721 ctaatcattg ttgggccaga aggaggtgag atgctgaaag ttcctgaatc tctggagtac
     3781 gccgtcagat agtagcgaca acaaacatag gaggaaggca tgaagatagt acggaatgca
     3841 ttagcggtca ttttcgccgc atgtgtgagt gcggcatacg ctgcagatgt gaccctcgaa
     3901 gtgaccgatc tgaaggacaa cgtgatcgtt ctacaacagg cacgtgcata tcaagaatcg
     3961 tccagcacca ttccgtcgaa ggtagtcacc ttcgacggaa tcgagatgga acttggttcc
     4021 ggccgggttg tgatt
//
LOCUS       DAOQUC010000176         4031 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_25084_length_4031_cov_3.300050, whole genome shotgun sequence.
ACCESSION   DAOQUC010000176 DAOQUC010000000
VERSION     DAOQUC010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4031
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25084_length_4031_cov_3.300050"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(353..1504)
                     /locus_tag="PKH07_04295"
     CDS             complement(353..1504)
                     /locus_tag="PKH07_04295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34198.1"
                     /translation="MKKVQFLSMIFLGLCLTASAAFAGTNWHDWFDGYANQAALEANW
                     PAAGGYAAGQLDTARVLSTAQSMRCEQSPTATRPTRSLQDSGAAQNGTLTLWVYDDGA
                     DLKQFDVRINQNAPAYYVALGLRDDQTGSQTNYTCNINGTVSSTGIPRSTGWHLFQIQ
                     CFAGATTMKIDGKTFAGNSATGMKGDLVTIDTTFGSSANQVWVDSAGWMYTTAADRNL
                     SAVSETYILDYGFEKDAANWSGVGTNSTFHAKDDGCQSIAGCLDSHNGSGTGLPYAFQ
                     GSVTATVAQGGSYKMGFYYMNGPHSFDGINDLSVSVNGQGLVNLGSGNLATDQWVYSY
                     GETGTFTLGPGSNALTIAVGGTNQGANSLARWDHFNLILVTGIEEWSQY"
     gene            2086..3573
                     /locus_tag="PKH07_04300"
     CDS             2086..3573
                     /locus_tag="PKH07_04300"
                     /inference="COORDINATES: protein motif:HMM:NF019325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HNT34199.1"
                     /translation="MGAFRVISREYLYVLVSVCLLTATGLLENAAAQSGQSPKPTPTP
                     LFSFRIALTATPMPDLVIPQEDLKLDPVEPFEGRTLFVRFTVKNVGNGRAEDITVDAY
                     QRKADDPQAVPQTLTTSMHPDPIPFLAPGQSAELTMRWDAFDNAGNHSIEVIVDPQNR
                     IQESNETNNSTSISFHVTPNYLALTEEGNWFLSQARQRLRQAVKQVEAYREWAPGDPR
                     FLEELDHQFLVYLHLVDEDVAIAEKKAREALTVFPKNVPLSYLLASALLLREATSEAL
                     DVIESVSTTWGSTEDQLLLRVLREKDQDAAEFAKWKNSTDIKDVEMFLRTSVERLPGS
                     PYSWLQLGLVLLDRGKHSEACEAFDRSFFLTRGKIYTVTSDWTLFNWTRALHAMRRDQ
                     EALEHLAWGIKHFKRPFNLLFQYATVGFESKLVSPHVALHFVDQIMDSQETPFWMTRL
                     LYLRGRILEQLDLHEEAESAYRMSLLQDASYEPSIKALDILGATP"
     gene            3608..>4031
                     /gene="bshC"
                     /locus_tag="PKH07_04305"
     CDS             3608..>4031
                     /gene="bshC"
                     /locus_tag="PKH07_04305"
                     /inference="COORDINATES: protein motif:HMM:NF021586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis BshC"
                     /protein_id="HNT34200.1"
                     /translation="MIFRDLPVAFKQMPSAARGIVCYAQGDLRGILRTNEVPWDALVK
                     QRRGHPCLMSPALAQVIAQSQNFWGAGSKSARNAEALAESGTFCVVTGQQPGLLTGQL
                     YTLFKVSTAVAVARRVEEAVPGCRVVPVFWNASEDSDTE"
BASE COUNT          936 a         1079 c         1026 g          990 t
ORIGIN      
        1 cgggaagcag tgcccgaaca gctcttcgga cgccatccca aaaccattcc gcgcttgtgg
       61 tggcgctttg aggcgtcttc ttctcgtgct ggggttgtgt gttgttcttc tttctttttg
      121 ccatggttct tctgagaaat tacgcgcagc gtttcccttt ggcaagggag aaaggtctga
      181 tcctctttgt atgggttcga ccttcaggtc gtcttggaag acaacgcgcc gaagcgcgaa
      241 ctcttaccag aactttgacc gctgctgcca cacctgagag tgagggcgga aagaaaaagg
      301 gactgagcgc aaggcccagt ccctttcgta aacagatagc cgctcacgcg gtctagtact
      361 gactccactc ttcgattcct gttacaagaa tcagattaaa gtgatcccat cgtgccagac
      421 tgtttgcgcc ctggttggtt cctccaacag cgatcgtgag cgcattggaa ccaggaccca
      481 atgtgaacgt tccggtctct ccataactgt agacccattg gtctgtggcc aagttgcctg
      541 aaccgagatt caccaaaccc tggccattga ccgaaacaga gaggtcattt atgccgtcaa
      601 agctgtgggg cccgttcatg tagtagaatc ccatcttgta acttccacct tgagcaacgg
      661 tggcagtgac actgccttgg aaggcgtagg gcaagccagt acccgaaccg ttgtgggagt
      721 caagacaacc agcaatactc tggcagccgt catccttcgc atggaaggtc gagttagtac
      781 ccacgccaga ccagttggcg gcatccttct cgaagccgta gtccagaatg taggtctcac
      841 tgacagcgga gagatttctg tctgccgccg tagtgtacat ccagcctgca ctatccaccc
      901 atacctggtt ggctgaagag ccaaaggtgg tgtcaatagt caccaagtca cccttcatcc
      961 ctgttgcact gtttccagca aaggtcttgc catcaatctt cattgttgtg gcgccggcga
     1021 aacactgaat ctggaacaga tgccatccag tcgaacgcgg gattcctgta cttgatacgg
     1081 taccattgat gttgcaggtg tagtttgtct gacttccagt ctgatcatct cttaagccaa
     1141 gtgcaacata gtatgcgggc gcattctggt tgattcggac atcaaactgt tttagatccg
     1201 caccatcatc gtagacccaa agagtaagcg tcccattttg agcagctcca ctgtcttgaa
     1261 ggctgcgtgt agggcgtgtt gctgttggag actgttcaca cctcattgac tgagcagtgg
     1321 acaaaaccct tgccgtatcg agctgaccag cagcatagcc acctgccgca ggccagtttg
     1381 cttcaagggc tgcttggttg gcgtaaccgt caaaccagtc gtgccaattg gttcccgcga
     1441 aggctgcgga agccgtcagg cacaaaccca aaaagatcat agaaagaaac tgtacttttt
     1501 tcattgttca atgactccta atcctaagac ttctttcgag agatatccat tcgctgcctc
     1561 tggcacacgg ccgcgttcag attccctgta gtcagctgga tatccctgac tctgctttca
     1621 atagaaagcg cttacaaact tactaacaaa aaaacactca cctccttctc ccgcaccatg
     1681 tatttttgcg gattatcgcc cttcgtgaat gttcttgtca agactttttc atgaaatggc
     1741 gtatcagctc agttacacaa ggtattgttg tcattctgag tgcagcgacg ctctgggagc
     1801 ggaatgaaga atctctcttt actgcagaat ctagagagat tcctcactcc ttcgccaagc
     1861 ctcagtcgtt cggaatgaca gcctccgccc ttgtgtaact gagccaatac gaaatggcag
     1921 gattcaagga acatggcaca gtctccaatg gagaggcctg gcacatgtgc tcttatccaa
     1981 gccgcatttg cagagtcctt tttttggttc tatagttctg caaaaggcct cggaagatca
     2041 tctccagcga tgttttcggg gacagcaaga cacggagacc gttgtatggg cgcatttagg
     2101 gtcatatcga gagaatatct ctatgttctc gtttcggtct gtctgttgac agcgactggc
     2161 ttgttggaga acgcagcagc gcagtcggga cagagtccca aacccacccc gacaccactt
     2221 ttctcattcc gtattgcact gacggcaacg ccgatgcccg atctcgtcat cccacaggaa
     2281 gacctgaaac tggatcctgt cgagcctttt gaaggacgca ccctctttgt gcgcttcacc
     2341 gtcaagaacg tcggcaacgg acgcgcagag gacatcaccg tggacgccta tcagcgaaag
     2401 gccgacgatc cgcaggctgt tccccaaacg ctgaccacct cgatgcatcc cgatcccata
     2461 cccttcctcg ccccgggtca gtcggctgaa ctgaccatgc gttgggatgc ctttgacaac
     2521 gcgggaaacc actctatcga agtgatcgtt gaccctcaga accgaattca ggaatcgaac
     2581 gagacaaaca acagcacgtc catttcgttt cacgtgacac caaattacct tgccctgact
     2641 gaagaaggga actggtttct ttctcaagcg agacaacgcc tcagacaagc ggtaaaacag
     2701 gtggaggcct atcgggagtg ggcacccgga gatcctcggt tccttgagga actcgatcat
     2761 cagtttctgg tctatctgca tctggtggac gaggatgtgg ctattgccga aaagaaagcg
     2821 cgggaagccc tgaccgtatt tccaaagaat gtgcctctgt cctatctgct tgcaagcgct
     2881 ttacttcttc gtgaggcaac ttcggaagcg cttgatgtca ttgagagtgt ttcaacgaca
     2941 tggggcagca cagaagatca acttctgctg cgggtgttga gggaaaagga tcaggatgcg
     3001 gcagagttcg ccaagtggaa gaattcaact gacatcaaag atgttgaaat gttcctccga
     3061 acctcggtgg aacgtcttcc ggggtcgccg tacagctggc ttcaactcgg tctggtgctt
     3121 ctggatagag gaaagcactc tgaagcatgt gaggcattcg atcgctcctt ctttctcacc
     3181 cgcgggaaga tttacaccgt cacaagcgac tggactttgt tcaactggac tcgcgcgctt
     3241 catgcgatga gaagagatca ggaagcactc gaacatctgg cttggggtat caagcacttc
     3301 aaaagaccct tcaacctctt gtttcaatat gccaccgttg gtttcgaatc caagcttgtc
     3361 tcacctcacg tagcacttca ttttgttgac caaattatgg acagccagga aacccctttc
     3421 tggatgaccc gactgctgta tcttcgaggc aggatactgg agcagcttga tctgcatgag
     3481 gaggcggaat cagcatatcg gatgtctctg cttcaggatg cctcctacga gccgagcatc
     3541 aaggcgctcg acatcctggg agccacaccg tgaccttgat ggtaacccta tgggaaaagg
     3601 attgtctttg atattcagag atcttcccgt tgcattcaag cagatgccca gtgcggcacg
     3661 tggaattgtc tgctacgcac agggcgatct gcgcggtatt ctccgaacca acgaagttcc
     3721 ttgggatgct ctcgtcaagc aacgacgtgg acacccttgc ctgatgtctc cggctcttgc
     3781 tcaagtcatc gctcagagtc agaatttctg gggcgctgga agcaagtctg ccagaaacgc
     3841 tgaggctctt gccgagagcg gtactttctg cgtggtgacg ggtcagcagc ctggtctctt
     3901 gaccggacag ctctacacgc ttttcaaggt ttctacggca gttgcagttg cccgacgtgt
     3961 ggaagaggca gttccgggat gccgtgtcgt tccggtcttc tggaatgcgt ccgaagacag
     4021 cgatacagag g
//
LOCUS       DAOQUC010000177         4027 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_25116_length_4027_cov_2.667170, whole genome shotgun sequence.
ACCESSION   DAOQUC010000177 DAOQUC010000000
VERSION     DAOQUC010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4027
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25116_length_4027_cov_2.667170"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3257)
                     /locus_tag="PKH07_04310"
     CDS             complement(<1..3257)
                     /locus_tag="PKH07_04310"
                     /inference="COORDINATES: protein motif:HMM:TIGR03296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M6 family metalloprotease domain-containing
                     protein"
                     /protein_id="HNT34201.1"
                     /translation="MPFRYPFRGLFRCIGVTLAVLVTALLVSPQLSEALSVRPGVSTP
                     ENLLTLRDGVGANFNNYRGRNAFSMAPALRQSTLPQPDPLQGNVRALVLMVNDVGGPE
                     AFVTTIQVHRERLFSEGRYNNPSTGRAGSVRDYYLENSHGMLQFEGEIWGPTNIVFDA
                     DNNGAVSGAEAAGMIAQALNDVDAFMDLSKFDNDGPDGISRYDGSTDDDGNVDMLIVI
                     HAGLASQASGLASDPPTVMFDFGIPLATNDGVVVNEAILVPELGFYIDWNLFALGITV
                     GDIGGIVHEMGHLFGLPDLYDVDNSSEGIGEWGLMGSGSWLGIAYNNIEYPGTSPAHL
                     SSWSKYQLGWLNNKIQRLDTSGIYNLDAIATPPDLLPQCFHFWRPRSTGFPEYFLCEY
                     KVNKPNAGAVPFSYSTYIFDSTHPLGIEDRGVVVWHIDDSIGSFANNDANVIDFHPRV
                     MVVQADGRYDLQMRVNRGDSGDPFTHENIPPRDLLPDESSQDPTTYSYDGTPTGISMR
                     TVAFSADSATLEFTFKNVAPRIVINKPNSPLDITRRNIFQILWWDADPDDDARIELYY
                     STSSTLASVAQIRALAKAIVGPGSALSEDEESDRYDWDTYKDGPSGTGGYVPDGQFYI
                     YAVISDPVTTNWNVSRAPLTVRHDMPPTLEITKPYKIRDLADTSYEIRFKPWDDMVKG
                     TTTVELWATSTTAGGPAGGVQIMPPTNLSSFSGDFRYVWDTTSMVGQIYHIYGVIKDQ
                     EWNPVVSQFSPGSILVDHKPKFTFMKPCVGCMNDATGTYTIAWQASDEESPAQINLFY
                     SRVNNTSVNATQINTIPLIEGVDATYTWDFRLPFSVVGVGDYYIWARVDDGINPAQWF
                     VSPVPVNIVEAVGANVALNNLVQVGQRIDSQSPPTAVIGMNLSDDGLPLTLDSIRVEF
                     EDVSLINKIDPAVDFLPITGDSVGGIGLYRDSPTGLLDGAFDSLDTPVPFRFTLISPK
                     QFLLIPDPPLAVPDSDSIISVTDSATGQTEYNLGDDLYVVVRTSENIELNDGFRARIP
                     AGGLSFNNGRNMTAQVLTAPLLANVPTFLTDLSPEDGVLRERHQAYPIIGIGMADG"
     gene            complement(3380..>4027)
                     /locus_tag="PKH07_04315"
     CDS             complement(3380..>4027)
                     /locus_tag="PKH07_04315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34202.1"
                     /translation="FIVAAAMDGTVNVVDASGTIVASRRLPDNQHIYLSPVVADLAHP
                     SEMASNGVQEIVVATYEGQVYALRSDLSDAFGAPGAAPQPFAQVSASITTCPSIGDFD
                     GDDEAEVVFGDSDFKIRVWDVVGAGFSTATNAWPMYRHDPLHTGLLPNRLVPVPMDLN
                     GDGIVDWADLFILSQNWRSLDTSGLQGIEDLNQNQMLDGVELLYFVEQWHNLSSK"
BASE COUNT         1005 a         1149 c         1020 g          853 t
ORIGIN      
        1 ccatccgcca taccgatacc gatgatcgga taagcctgat gtctttctct caaaacgccg
       61 tcttcaggag agagatccgt caggaaggtg ggaacattcg ccaacagcgg cgctgtaagc
      121 acctgtgcgg tcatgttgcg tccattgttg aacgacagac cgccagcggg aattctggct
      181 ctgaagccgt cattgagctc aatgttttca ctggtgcgaa caacaacgta cagatcgtcg
      241 cccagattgt actccgtctg tcccgtggcg ctatccgtta ccgagatgat cgaatcgctg
      301 tccggaaccg ccaaaggcgg atccgggatc aagagaaact gcttcggcga tatcagcgta
      361 aaccggaacg gaacaggggt atcgagactg tcgaacgcac catccagaag tcctgttgga
      421 ctatcccgat agagcccgat gcctcccaca gaatcccccg tgataggcag gaagtcaacg
      481 gctggatcga ttttgttgat cagactgacg tcttcaaact ccacgcgaat cgaatccagc
      541 gtcaatggca gcccgtcatc actcaggttc atgccgatca cggcggtggg cggtgactgt
      601 gagtcaatac gctgtccgac ctgcaccagg ttgttgagcg cgacgtttgc gccaaccgcc
      661 tcgacaatgt tcaccggcac tggactgaca aaccactgtg cagggttgat cccatcgtcc
      721 acgcgcgccc agatgtaata atcaccaaca ccaacgacac tgaaaggcaa ccggaagtcc
      781 catgtgtaag tcgcatccac accttcaatc aatgggatag tgttgatctg tgtcgcgttg
      841 acagaagtgt tattgactcg gctgtagaag agattgatct gcgccgggga ctcttcatcc
      901 gaggcctgcc atgcgatggt ataggtacct gtcgcgtcat tcatgcagcc cacacaaggc
      961 ttcatgaaag tgaacttcgg tttgtgatcc acaagaattg aacccgggct gaactgggaa
     1021 acgaccggat tccactcctg atccttgatc actccgtaga tatgatagat ctgaccgacc
     1081 atgctggttg tatcccagac atagcggaag tcgccgctga aggaacttag attcgtcggc
     1141 ggcataatct gaacgccacc ggctggcccc cccgccgtgg tcgaggtcgc ccacaattcc
     1201 acggtcgtag tccccttgac catgtcatcc cacggtttga accgaatctc ataacttgtg
     1261 tccgcaagat ctcgaatttt gtagggcttc gtgatctcca acgtcggcgg catgtcgtgg
     1321 cggaccgtca agggagcacg actgacattc cagtttgtcg tcaccggatc actgatcacc
     1381 gcatagatat agaactgacc gtcgggaaca tagccacctg tcccactcgg accatcttta
     1441 taggtgtccc aatcgtatcg gtcggactct tcatcctcgc tcagggcgga gcccggtccg
     1501 acaatcgcct ttgccagggc gcgaatctgc gcgacgctcg caagcgtaga actggtcgag
     1561 tagtacaatt cgatccgggc atcatcatcc ggatcagcgt cccaccagag gatttgaaag
     1621 atattgcgcc gagtgatatc gagcggggaa ttcggcttgt tgatgacaat acgcggcgca
     1681 acattcttga acgtgaattc caacgttgcg gaatccgccg agaacgctac cgttctcatg
     1741 gagataccgg taggtgtgcc atcataggaa taggtggtcg gatcctgaga agactcatcg
     1801 ggcagcagat ctctgggcgg tatattctca tgcgtaaagg gatcgccact atcaccccta
     1861 ttgactctca tctgcaggtc ataacgtccg tccgcctgca ccaccatgac ccgaggatga
     1921 aagtcaatga cgttcgcgtc gttgttggcg aaactgccga tagaatcatc tatgtgccag
     1981 accactacgc ctcgatcctc aatacccaga ggatgggtcg agtcgaagat atatgtgctg
     2041 taggaaaaag gtactgcacc cgcattgggt ttgttgacct tgtactcaca aaggaaatac
     2101 tccgggaatc ctgttgaacg cggtcgccaa aaatgaaaac actgaggaag aagatcgggg
     2161 ggcgtcgcga tggcatcaag attgtagatc cctgatgtat caagtctctg aatcttgttg
     2221 ttgagccagc ccagttgata cttcgaccaa gaggatagat gcgccggtga cgtgccggga
     2281 tactcgatgt tgttgtacgc gattcccagc caggaaccac tgcccatcag tccccattca
     2341 ccgatacctt cactgctatt gtccacgtca tagagatcgg gaagtccgaa cagatgtccc
     2401 atctcatgaa cgatcccacc aatgtcgcca acggtaatac caagtgcaaa aagattccag
     2461 tcaatgtaga aacccaattc tggaacaagg atcgcttcat tgacgacaac accgtcattg
     2521 gtcgccagag gaatgccaaa gtcaaacatc accgtcggcg gatctgaagc cagtcctgag
     2581 gcctgtgaag caagtcccgc gtggatcacg atgagcatgt caacattacc atcgtcatcc
     2641 gttgaaccgt catagcgtga gataccgtcc ggcccgtcat tgtcgaactt ggacagatcc
     2701 atgaaggcat ccacgtcgtt caaagcctgc gcgatcatac cggcagcttc agcaccgctg
     2761 accgccccat tgttgtccgc gtcaaagacg atattggtag gtccccaaat ctctccttca
     2821 aattgaagca tcccgtggga attctcgaga tagtagtcac gaacactgcc cgcacgccct
     2881 gttgaggggt tattgtaacg gccttcggag aaaagcctct cgcggtgaac ctggatcgta
     2941 gtgacaaagg cctctggccc cccaacgtca ttcaccatca agaccaacgc ccgaacgttc
     3001 ccctgcagag gatcaggctg aggcagggtg ctctgcctga gtgccggagc catcgagaag
     3061 gcattccttc cccgatagtt gttaaagttc gcaccgacac catcccgaag agtgagaaga
     3121 ttctccggtg ttgaaacacc gggtctcaca gaaagggctt ccgacagttg cggggaaacc
     3181 aacagggctg tcaccaagac cgccagcgtt acgccaatac agcgaaacaa accccggaac
     3241 ggataccgaa agggcattct attgtcctcc ttgataccat caaagcaacc aatcatctct
     3301 taaactgact ccgcaagaga atctctttca tgagcaagcc ctgtgaacga gcgggcagag
     3361 cccacacgct tccaggtgcc tacttgctcg aaagattatg ccattgctcg acgaagtaaa
     3421 gcagctccac tccgtcaagc atctggtttt gattcaaatc ctcaataccc tgcaaaccac
     3481 tggtgtccaa agaacgccag ttctgtgaca ggataaacag atccgcccag tccacaatcc
     3541 catcgccgtt caaatccatc ggtacgggca ccaaacggtt gggaagaagc cccgtgtgca
     3601 acggatcatg gcgatacatc ggccaggcat ttgtggctgt ggagaatccc gctcccacaa
     3661 cgtcccagac acgaatcttg aaatcagaat caccaaagac cacttctgcc tcgtcgtcgc
     3721 catcgaaatc cccaatcgac ggacaggtcg tgattgaagc actgacctga gcgaacggct
     3781 gaggagcggc tccaggagca ccgaaggcgt cggacaggtc actgcgaagc gcatagactt
     3841 ggccttcata ggtcgcaacg actatctcct gtactccgtt ggatgccatc tccgacggat
     3901 gcgccagatc ggccaccaca ggcgagagat aaatatgctg gttgtctgga agccttcgcg
     3961 aagcgactat cgtccccgaa gcgtccacaa cgttcaccgt accgtccatc gccgctgcca
     4021 cgatgaa
//
LOCUS       DAOQUC010000178         4009 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_25270_length_4009_cov_3.616591, whole genome shotgun sequence.
ACCESSION   DAOQUC010000178 DAOQUC010000000
VERSION     DAOQUC010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4009
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25270_length_4009_cov_3.616591"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            410..1138
                     /locus_tag="PKH07_04320"
     CDS             410..1138
                     /locus_tag="PKH07_04320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34203.1"
                     /translation="MVRILRLFVLIVLPVGLNSAFAERSDLIRFKSVYPHIIVTRLDS
                     CAVCHVSPVPSDGALNVYGADYKNAGSSELALVDIEQLDSDGDLISNVIEIERLTFPG
                     DSSDFPILTATPTDTPNNETPTIAPSDTPTDTMTATPTRTPSHTPTHTLTYTPTATAS
                     HTPTNTLTHTATLTCSCTPTPSATPTNTQTETPSETPTSTPPPQSRFDFNTDGSIDSL
                     DLLLLMQRPDAGELLFEFLLYWGL"
     gene            complement(1123..1719)
                     /locus_tag="PKH07_04325"
     CDS             complement(1123..1719)
                     /locus_tag="PKH07_04325"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HNT34204.1"
                     /translation="MTVSHSHIINDLSELSGSKVTLRPVTEDMAEDMFEATSESQTEL
                     NPFMPWENQVVQDAKDFIERSARSREQGEELQLAIFERETDEFVGMIGLFQLDPFTPK
                     GTIGYWIRTSKAGSGFATDAVSTLLAFCQQRLDLARVDACVASGNPASQRVLEKCGFR
                     REGLKERAELCHGQWHDVVMYGIVFPYDQKQKDSHSPQ"
     gene            1917..2501
                     /locus_tag="PKH07_04330"
     CDS             1917..2501
                     /locus_tag="PKH07_04330"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNT34205.1"
                     /translation="MSDDRKETKREDEILISRFLGGDTSAFDALVRKYRDRVIHIAYR
                     VVQDQDEAVDVSQEVFVKVFRSLNRFSGKSSFYTWLYRIATNLAIDHRRRSKRILEFR
                     DELKTDDDESETQLVAKVFDPRDKAIDEQLEKHIHEEINELPEHHRMVVLLRDVEDYS
                     YAEIAEILGCSIGTVMSRLHYAREKLQKKLQKYL"
     gene            2750..3271
                     /locus_tag="PKH07_04335"
     CDS             2750..3271
                     /locus_tag="PKH07_04335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34206.1"
                     /translation="MRCKEFRNYVDRYLDQEIREPELVAQLDEHSRCCNDCASMLDAR
                     GKLVRSVRGMGRETLTLPPMEIYLAELREKIAHPSQQSIWERLRTSIVPSHAYAFGGF
                     VLAMLVIAVFVQYVGNPSLNVAMPNQASYIEYLEVPEHRVSAVTYTNAQTRTTIVWLC
                     GAEVRATTSEGRS"
     gene            3268..3777
                     /locus_tag="PKH07_04340"
     CDS             3268..3777
                     /locus_tag="PKH07_04340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34207.1"
                     /translation="MIFTLSHIKPRGVVLLMIISLLLAGVSPVSLQAQSQSPREAQKQ
                     SITLTVRMIQASDSDAKERTIPKELEDLKEDLSSFPYKVFSQLSLRSFRATIGSAATT
                     GLAHGLQLQVTPSGSEGDMHSLAIRLIEGRDRVLLNMTLRTRSGSYTLIGGPKIGENA
                     VVVLAISAK"
BASE COUNT          926 a         1076 c         1065 g          942 t
ORIGIN      
        1 cgggatcgaa agccaccctg gcctggagca ctacgggctg tacgacactg tggtcgcgag
       61 ggaggatgcc tgaagagact gcgggctaac aacagcatgc agacggacaa gactacgcgc
      121 cgccactgat gtttgacgag agatcggctc gcacaccatg ccactacaag gtctgaatcc
      181 tgacgacaga gttgttgcac ttgccgagct accagattcg ccgacgggtt tcggactgca
      241 ggagagactc ctcactcctt cgccaagcct cagtcattca tgacagttcc gctctcgtgt
      301 aactgagcca acacttcttc ggctgcactt gcagttgcca gaacggcagg caattcgata
      361 tcatcacggc agtctgtgga aggtcatggc agatgtggag gatacacaag tggtaagaat
      421 actccggttg ttcgtcctga ttgttctacc ggtgggactc aattcagcct ttgcagaacg
      481 ctcagatctg atccggttca agagcgtcta cccgcacatc atcgtcactc gtttggactc
      541 ctgtgcagtc tgccacgttt cgcccgttcc ttcggatgga gcattgaacg tctacggtgc
      601 cgactacaag aatgcgggtt ccagtgagct ggcactggtg gacatcgagc aactggactc
      661 cgacggagat cttatctcta acgtcattga gatcgagcgt ctcactttcc cgggagattc
      721 ctccgacttt cccatcttga cggcgactcc gacggacacc ccgaacaacg agacacccac
      781 catagcgccg agtgacaccc cgacagacac gatgaccgca acgcccacaa ggactccgag
      841 tcacactccg acccatacac tgacctatac gccgactgcg acagcaagcc atacgccaac
      901 aaacactctg acgcacacag caacgctgac ttgttcctgc accccgactc cgtcagccac
      961 ccccacaaac acccagacag aaacgccaag cgaaacgccc acctcaactc ccccacccca
     1021 gtcacgattc gatttcaata cagatggttc cattgacagc ctggatcttc tgctgctcat
     1081 gcagaggccg gatgcgggag aactgctctt tgagttcttg ctctattggg ggctgtgact
     1141 atctttctgc ttctgatcgt acgggaagac gattccatac atcaccacat catgccactg
     1201 cccgtggcat agttctgctc tttccttcaa gccttcgcga cggaatccac acttttccag
     1261 aactcgctga ctcgcaggat tcccgctcgc gacgcaggca tctacccgcg ccaagtccag
     1321 ccgctgctga cagaaagcca gaagcgtgga gaccgcgtct gtggcaaagc cgctgccagc
     1381 ctttgatgtt cgaatccagt agccgatcgt tcctttcggt gtgaacgggt ccagttgaaa
     1441 gagtccgatc attccgacaa actcatccgt ttctcgttca aaaatggcca gttgaagttc
     1501 ctctccctgc tctctggagc gggcggatct ttcaataaag tctttggcgt cctggacaac
     1561 ttggttttcc catggcatga atgggttcaa ctcggtttgg gactcagatg tggcctcaaa
     1621 catgtcctca gccatatcct ctgtgaccgg tcgcagggtg accttgcttc ctgacaactc
     1681 actgagatca ttgatgatgt gtgaatgaga aactgtcatc ttgggaggcc tttctgggga
     1741 tcgaacacgc tgcaaaatgc agcacccggc cgatttatgg actgagcata gtccatctcg
     1801 aagtggtttg caatggcgtg aatccaagcc cacagtttcc agttcattgt tcgtagtgga
     1861 aactcgatgc cggatggcat atcattcgaa tatacgtcaa ttcaggagcg tcaaacgtgt
     1921 cggatgacag aaaggagacc aagcgcgagg atgaaatcct catcagccgt tttctgggtg
     1981 gcgatacgtc agcctttgac gctctggtaa ggaaataccg agatcgagtg attcacatcg
     2041 cctatcgggt tgtgcaagat caggatgaag ccgttgacgt ttctcaggag gttttcgtca
     2101 aggtttttcg gtctctgaat cggttttcag gaaagtcttc gttttacact tggctgtatc
     2161 gaattgcgac gaatctggca attgatcatc gaaggcgatc caagagaatc ctggagtttc
     2221 gggatgagct gaaaaccgat gacgatgagt cggagacgca actggttgcc aaggtgttcg
     2281 accctcgtga caaggccatt gatgagcaac tggagaagca cattcacgag gagataaacg
     2341 agttgcccga acaccacagg atggtcgtgt tgctccggga tgtcgaggac tacagttacg
     2401 cggagattgc agagattctc ggatgctcga ttggaacggt gatgtcgcgc ttgcattacg
     2461 cccgagagaa gttgcagaag aagttgcaga aatacctctg atgcgacgct tggtcgaagt
     2521 gggttgttgg attagagggc acatggagag aatgatgagc cagtcagcgc gcagtgcaga
     2581 gaagagacca aaactgctgg ctaatgcttt tttttcgaag gagttcccag tcttgtccgt
     2641 ctatactggc ggaggtgata gagttctctg gcgagtcttg tcaaatgaag gcctttgtgc
     2701 ttcactatca tcagcctgaa aaggggaccc cgagaagggg gtgttcgcga tgagatgcaa
     2761 ggaatttagg aattacgttg accgctacct tgatcaagag atcagggaac ccgaactggt
     2821 ggctcagctg gatgagcaca gtcggtgctg caacgattgt gcttccatgt tggacgctcg
     2881 tggaaaactc gttcgttccg ttcggggaat gggcagggaa acgctcacgc ttccgccgat
     2941 ggagatctat ctggccgagt tgcgcgagaa gattgctcat ccatctcaac agagcatctg
     3001 ggagcgtctg cgaacgagca ttgttccatc acatgcctat gcgtttggcg ggtttgtcct
     3061 tgctatgctt gtcatcgcgg tctttgtcca gtatgtgggc aatccatccc tgaacgtggc
     3121 aatgccgaac caggccagtt acattgaata cctcgaggtt cccgagcatc gggtttccgc
     3181 ggtcacctat accaacgctc agacccggac gaccattgtc tggttgtgcg gggctgaggt
     3241 cagagcaacg acgtcggagg gtcgctcatg atttttacac taagtcacat caagccccgc
     3301 ggcgttgtgc tgctcatgat catttctctc ttattggcag gtgtgtcacc cgtttctctc
     3361 caggcgcaga gtcagagccc acgagaggct cagaaacagt caatcacctt gactgtccgc
     3421 atgattcaag catcggactc tgatgcaaag gagcgaacga tccccaagga gctggaggat
     3481 ctcaaggaag atctttcctc gtttccctac aaagtcttct ctcagctgag cctgcgctct
     3541 ttccgagcga ccataggttc cgcagcaacc accggtcttg cgcacggtct ccagctgcaa
     3601 gtcactcctt ccggctcaga aggagacatg cactccctgg ccatacgcct catagaaggt
     3661 cgcgacagag ttcttctgaa catgacactc agaactcgat ctggcagtta tacactcatc
     3721 ggcggcccca agattggtga gaacgcggtc gttgtgcttg ctatctcggc gaagtaattg
     3781 aacgcaggat gaattcgtcc tctctgactg aaaagcagtc tgaatcctct tggcaaaagg
     3841 tgaaacaatg gcagtaggaa tcggcatcct cggttttgcg catggacatg tggaaatgta
     3901 ctgtgctcgc tggcgtcaaa tgttctcaga ccgaattcgt cttgtggcgg gttgggatca
     3961 tgattctgcc cgcgcttcgg ccgcgtgtga gagacatggc ttgtccgtt
//
LOCUS       DAOQUC010000179         4002 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_25348_length_4002_cov_2.875095, whole genome shotgun sequence.
ACCESSION   DAOQUC010000179 DAOQUC010000000
VERSION     DAOQUC010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4002
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25348_length_4002_cov_2.875095"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            169..546
                     /gene="queD"
                     /locus_tag="PKH07_04345"
     CDS             169..546
                     /gene="queD"
                     /locus_tag="PKH07_04345"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HNT34208.1"
                     /translation="MYRISVEMGFSAAHQLVGYEGACENLHGHNWTVKVEVKTTKLDS
                     LGMGLDFKKLKKLLGGVLEQFDHRMINQVAPFDQFNPTAENMAHFIFDALKSRLPKDV
                     SMSRVKVSESPQCSVIYQEDSAE"
     gene            543..1178
                     /locus_tag="PKH07_04350"
     CDS             543..1178
                     /locus_tag="PKH07_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007222377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNT34209.1"
                     /translation="MKVVEVFHSIQGESTHAGLPCVFVRLSGCNLRCSYCDTQYAYDE
                     GERMSLDEILVRVQRYDCPLVEITGGEPLLQEQTPQLTSQLAHKGYTVLVETNGTVDI
                     SGLDKRAIRIMDIKCPGSGCSDKTLWSNVELLRKTDEVKMVLCDERDYLWARGIIEKF
                     NLLERTTVLLSPVHGRLEPAQLAAWILRDRLNVRLQLQLHKYIWNPETRGV"
     gene            1182..1529
                     /gene="queF"
                     /locus_tag="PKH07_04355"
     CDS             1182..1529
                     /gene="queF"
                     /locus_tag="PKH07_04355"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012507475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HNT34210.1"
                     /translation="MALVETFDNPRSDRPYTITITNPEYTSVCPKTGLPDFGTITIHY
                     TPDAKCLELKSLKYYFLEFRNKGIFYEAAVNAMLDDLVASCEPLKMEIIGDFSARGGI
                     TCTVKAVYEKSVR"
     gene            complement(1651..2076)
                     /locus_tag="PKH07_04360"
     CDS             complement(1651..2076)
                     /locus_tag="PKH07_04360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34211.1"
                     /translation="MQDIKQALYQRLLSDGTLTTLLGSTEPDPRVYSAEPPQQIPLSD
                     ATPAFITMTLASLGTPTGPDGVTAPDEVYDVRIWSDQPSVIESASSRLVSVLNGWMFD
                     TTEHCGMCVALLGSTDMIHAASGRYCRKQTWRVWHIMDA"
     gene            2365..2667
                     /locus_tag="PKH07_04365"
     CDS             2365..2667
                     /locus_tag="PKH07_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HNT34212.1"
                     /translation="MKKFKRVAEQFRQEFRVYQKVLRDPRTPLLPKILLGLAIGYFVL
                     PIDLIPDFIPFFGQLDDLVIVPVLVVLALKLIPREIIDEWRTRLRASQKDFDSTEA"
     gene            complement(2654..2977)
                     /locus_tag="PKH07_04370"
     CDS             complement(2654..2977)
                     /locus_tag="PKH07_04370"
                     /inference="COORDINATES: protein motif:HMM:NF017343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail adaptor protein"
                     /protein_id="HNT34213.1"
                     /translation="MAITSVLNQQATIHRKALTYSDSGEPQTAWTPIAVTVACAVEVS
                     GRRLWRGAGGWISKQTARAFFEPETDLRIEDRLIVGGETYLVAHVADAAGRGHHLEAL
                     LVKPL"
     gene            complement(3008..3226)
                     /locus_tag="PKH07_04375"
     CDS             complement(3008..3226)
                     /locus_tag="PKH07_04375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34214.1"
                     /translation="MTLNALELEAKIAELDTQLAALSQEPETAVDKRIGAYSEANSQK
                     YRMLSDLRNSYLDQLRKIPYEEVDHFAM"
     gene            complement(3314..>4002)
                     /locus_tag="PKH07_04380"
     CDS             complement(3314..>4002)
                     /locus_tag="PKH07_04380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34215.1"
                     /translation="EKYVQIDNTKYGRILSITEEAIQFDQTGQLLARASQFGVKARMK
                     RERIIQEGVIDANYSAGVSGVYKPSGTVTQLYSTTNGNLNTSAGTLNDTNLATAYAAL
                     RSMTDEEGDFIMPSSQMILMCHPAVFKTARVYANSTLIPGSSNNDVNFWKGMFLPVEN
                     PYIPEQTTWFLGDFAAQFRWQEVWPLQVMRAIAGNDFDFQRDIRAAFKVRFFGGVGAT
                     DYRFVQKQTA"
BASE COUNT          931 a         1125 c         1022 g          924 t
ORIGIN      
        1 gagactcttt gctccgcaga ctccgctcag agtgacatct cacttaaacg ggtgttccac
       61 tgcccccttc cgtaacagag ccagtacatc aatcccacca tcgagtttga ctcgtttgcc
      121 gagtccccta taatgagacc gcttagaaaa tgacgcgagg tgacaaccgt gtaccgaatc
      181 tctgtcgaaa tgggtttttc cgcagcgcat cagctggtcg gctatgaagg tgcctgcgaa
      241 aaccttcatg gtcacaactg gacggtcaag gtggaagtca aaaccacaaa actggattcg
      301 ctggggatgg gtctggattt caagaaactc aagaagcttc ttggcggagt tctggagcag
      361 tttgaccaca ggatgatcaa ccaggtcgct ccgtttgacc agttcaatcc gacagcggag
      421 aacatggctc atttcatctt cgacgccttg aaaagcagac tgcccaagga cgtctcgatg
      481 tcccgtgtaa aagtgtctga atcgccccag tgcagtgtga tctatcaaga ggattctgcc
      541 gaatgaaagt cgtcgaggtt ttccacagca tccagggcga atccacccac gcgggcttgc
      601 cctgcgtttt cgtacgcctc agtggatgca atctccgctg ctcctactgc gacacgcagt
      661 acgcctatga tgaaggcgaa agaatgtctc tggacgaaat cctcgtccgt gtgcagagat
      721 acgactgccc acttgtcgaa atcaccggtg gtgaaccctt gcttcaggag cagacacctc
      781 aactgacaag ccaactcgcg cacaagggtt ataccgtcct tgttgaaacc aatggaactg
      841 tggatatcag tggtcttgac aaacgcgcca ttcgaatcat ggacatcaag tgtcccggca
      901 gcgggtgttc cgacaagaca ctctggtcga acgtggaact acttcgaaag acggacgaag
      961 tcaagatggt gctctgtgat gaacgcgatt acctctgggc aagagggatc atcgagaagt
     1021 tcaatctgtt ggagcgaaca accgtcttgc tgtcaccggt tcacggcaga ctcgagccag
     1081 cgcaactggc agcatggatt ctgagagacc gtctgaacgt tcgtctccaa cttcagctgc
     1141 acaaatacat ctggaatccg gaaacaagag gagtatgaaa aatggcactc gtagaaactt
     1201 ttgacaatcc aaggtcagac agaccctata cgattaccat caccaacccg gaatacactt
     1261 ccgtttgccc aaagacaggg cttcccgatt tcggaacgat cacgattcat tacacgcccg
     1321 acgcgaaatg tcttgaactc aaatcactca agtactactt cctcgaattc cgaaacaagg
     1381 ggatcttcta cgaggcggca gtcaacgcca tgctcgacga cctcgttgca tcctgcgagc
     1441 ctctgaaaat ggaaatcatc ggagatttca gcgcccgagg cggcatcacc tgcaccgtca
     1501 aagccgtcta cgagaagagc gttcgataat gagtttctgt tgtagtgtgc tccatgtagg
     1561 ggcgctgctt gctgcgccct tccggagggc agggcaagcc ctgcccctac agaccgaacc
     1621 accccacaat ggaggatgct tcctgtgtat tcatgcatcc atgatatgcc acactctcca
     1681 cgtctgtttg cggcaatacc gtccactggc agcatgaatc atatcggtgc tgcccagcag
     1741 tgcaacacac attccgcaat gctcggtcgt atcgaacatc catccattca agaccgatac
     1801 cagtcgtgaa gaggcggatt caatgactga cggctgatca ctccaaatcc gcacatcata
     1861 gacctcgtcg ggagccgtaa ccccatcggg tcctgtgggt gtcccgagag atgccagagt
     1921 catcgtgatg aacgctggtg ttgcgtcgga caatggtatc tgctgaggcg gctcagcaga
     1981 gtaaacacgc ggatcgggct cggttgaacc gagaagtgtt gtgagcgttc catcgctcaa
     2041 gagtctttga tagagagcct gtttgatatc ttgcatctgc gcgcttttcc tttcccaact
     2101 cttgctggga gggcgggcat cttgccggcc attcctgacc atcgcgggcg agacgcctgc
     2161 gctccgtatc tctcatccgc tccttctccc agagcctgta gaccgacaaa ctccatagtt
     2221 ttgagaatct gtgaaatccg ctaatctgcg tcatcagcgg ttcagacatt cttcctatcc
     2281 gctgcaaggc gacacgcctg cgccacgttc tcccttgaca agaggaagtg gaaggggata
     2341 tttcctgctt cggacaagat ggaaatgaag aagttcaaac gcgttgctga acaattcagg
     2401 caggagtttc gcgtgtacca gaaggttctg cgcgatccgc gaacaccact gttacccaaa
     2461 atccttctcg gcttggcgat aggatacttc gtgcttccca ttgatctgat cccggacttc
     2521 atcccgttct ttgggcagct cgacgatctc gtgattgtgc ctgtcctcgt cgttctggcg
     2581 ctgaaactca tcccacgaga gatcattgat gagtggagaa cgcggctgag ggcatcgcaa
     2641 aaagatttcg attctacaga ggcttgacca gaagtgcttc gagatgatgc ccgcgaccgg
     2701 cagcgtctgc gacatgtgcg acaaggtacg tctcaccacc tacgatcaga cgatcctcaa
     2761 tgcgaagatc tgtctccggc tcaaagaatg ctcgggcggt ttgtttgctg atccaaccac
     2821 cggctccacg ccagagcctt ctgcccgaca cctctacggc acaggcaaca gtaactgcaa
     2881 taggcgtcca tgcggtctgc ggttcgcctg agtcagagta tgtgagcgcc ttgcgatgaa
     2941 tggtcgcttg ctgattcaga acgctggtta ttgccatcct ctcatctcct gtggttccga
     3001 tccccagtca catcgcaaag tgatccactt cttcgtaagg gatctttcgc aactgatcga
     3061 gataggagtt tcgcagatcg gagagcatcc gatacttctg actgtttgct tcagagtacg
     3121 cgccaatcct tttgtccaca gccgtctccg gttcctgtga cagcgccgcc agttgggtgt
     3181 ccagctcggc aatcttcgct tcgagttcca atgcgttgag agtcatggat tgctccttga
     3241 gtgggtctgc acaccgtaga cgaatcggac ggggcgggag ttgtgtctcc cgccccgtta
     3301 cgctccgcaa ggtctaggca gtctgcttct gcacaaaacg gtagtcagtg gctccaacac
     3361 caccgaagaa ccgcactttg aaggcagccc gaatgtcccg ctgaaaatcg aagtcgttgc
     3421 cggcaatcgc acgcatgacc tgaagtggcc acacttcctg ccaccgaaac tgtgcggcga
     3481 aatctcccag aaaccacgtg gtctgctcgg ggatgtacgg attctcaacg ggcaaaaaca
     3541 tacccttcca gaaattgaca tcgttgttgg aagagcctgg gatcagcgtt gagtttgcat
     3601 agactcgggc agtcttgaac actgccggat gacacatcag aatcatctgc gacgacggca
     3661 tgatgaagtc gccctcttca tccgtcatcg aacgcagcgc ggcataggcc gttgcaaggt
     3721 ttgtgtcatt cagagtgccc gcactcgtgt tcaggttccc gtttgtagtc gagtacagct
     3781 gcgtcaccgt gccactcggt ttatacactc ccgaaacacc agcgctgtag ttggcgtcaa
     3841 tcacgccttc ctgaatgatc cgttcgcgct tcatacgagc cttgacgcca aactggcttg
     3901 cccgcgccag aagctgaccg gtctgatcga actgaattgc ttcctcggta atgctgagga
     3961 tgcgcccata cttggtgttg tcgatctgaa cgtacttctc ac
//
LOCUS       DAOQUC010000180         3997 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_25399_length_3997_cov_2.983257, whole genome shotgun sequence.
ACCESSION   DAOQUC010000180 DAOQUC010000000
VERSION     DAOQUC010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3997
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25399_length_3997_cov_2.983257"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..480
                     /locus_tag="PKH07_04385"
     CDS             <1..480
                     /locus_tag="PKH07_04385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34216.1"
                     /translation="AEGHGISIERLSDDFLSSAGENSGVLATGCEAASLFKREDRYYA
                     LFDSCCCFCPQGSGARVYSATSPMGPYALHGNINRDEHTTPIIPAQQTHIAQLPSASG
                     DVFIWMGDLWGSRPDGIKGHDLQHWSSPMVFRPDGTIEPLKREESWSFVLRSAAVGR"
     gene            617..1426
                     /locus_tag="PKH07_04390"
     CDS             617..1426
                     /locus_tag="PKH07_04390"
                     /inference="COORDINATES: protein motif:HMM:NF015147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LEA type 2 family protein"
                     /protein_id="HNT34217.1"
                     /translation="MKKIFLVVCLCAVVSMGCSMAQIRALFGAIEKPTIESIRPRILG
                     LDFSGINLAFDVDLNNPNSFPLKTPKFKYGLDIQGSNFMKSEGTADIDLPAKGVGTVV
                     LPAKIKYLDIWKTYKGLKDANQVSYDLKGAFVVSPLGKDIELPMSKSGSFPVLKIPNF
                     SKISLEPSEVSLTESKVKVKANVTNPNVFEMGLDKLGYVLKMGDVDFADIKSTLSKSL
                     QAGETGQIELDAKVGGLSVLGRILDGVDMGKLSLTPEGSVTTPYGEIPLPK"
     gene            1499..2542
                     /locus_tag="PKH07_04395"
     CDS             1499..2542
                     /locus_tag="PKH07_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020267411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HNT34218.1"
                     /translation="MGLNFGLGALPMLSNAETRSICAENPNGERGAGAKEIPDAGNAG
                     AMLGQGWKVRPCITLEPGTTTTLADIKGPGVIQHIWITVDPKAYRDCVLRFYWDKEKT
                     PSVEVPLGDFFCNGHGIRHKVDSLAVAVNPSGGFNCYFPMPFRKHAKITIENQRHEAI
                     WGFFYQINYALTEVPRDAAYFHAQWRRSVTTREYPEHVLLDGIKGQGHYVGTFLAWTQ
                     MSTGWWGEGEIKFYLDGDSKFPTICGTGTEDYFGGAWCFGETYSTAFLGYPLWHKVEG
                     QVPKHDLYRWHILDPIRFKKNMKVTIQALGWWPNGKYEPLTDDIASIGYWYQVEPHAT
                     FPQFPAVHQRWPR"
     gene            complement(2626..2847)
                     /locus_tag="PKH07_04400"
     CDS             complement(2626..2847)
                     /locus_tag="PKH07_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002766797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HNT34219.1"
                     /translation="MPKFGPVSRRDLVKFLRQCGFDGPYSGGNHAFMIQGDLTLSIPN
                     PHVGDIGRDLLARILRQANISKQRWESLQ"
     gene            complement(2847..3095)
                     /locus_tag="PKH07_04405"
     CDS             complement(2847..3095)
                     /locus_tag="PKH07_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006419878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HNT34220.1"
                     /translation="MLSKYLEASMKHAHYELLEEDGSYYGEISLCPGVYANAQTLEAC
                     REELASVLEDWLLFRIHKNLSIPVIDGLELTVKKETAA"
     gene            3265..3387
                     /locus_tag="PKH07_04410"
     CDS             3265..3387
                     /locus_tag="PKH07_04410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34221.1"
                     /translation="MEPLLEILIEMILVNNDLIHHPEILPSEMRFSDQIGAADY"
     gene            3384..>3997
                     /locus_tag="PKH07_04415"
     CDS             3384..>3997
                     /locus_tag="PKH07_04415"
                     /inference="COORDINATES: protein motif:HMM:NF013703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HNT34222.1"
                     /translation="MSTATESSESSNRPMSVLVHRSVRQTLFRMAFPMLAGTFAMNAY
                     NLADTWFVSRLGTLPLAAMGFTFPVIMLLTCLASGLGTGVTTLVSHAIGRHDHDDAAR
                     IVTHGVIMTVSISLLISIAGYLSIDAIFARLGARGEVRILVGQYMRTWYLGALTMSLP
                     MLGNGILISSGDSKRASRLMILGTGLNVILDPIMIFGYFGFPAMG"
BASE COUNT          931 a         1017 c         1087 g          962 t
ORIGIN      
        1 gctgaagggc acggcatctc catagagcgg ctttccgacg actttctctc ttctgctgga
       61 gagaacagtg gcgtgctggc gaccggttgc gaagccgcct cactgttcaa gcgcgaagac
      121 cgctactatg ccctgtttga ttcgtgctgt tgtttctgtc cgcaaggctc cggagcgcga
      181 gtctactcgg caacttcacc aatgggaccc tacgccctgc atgggaacat caaccgcgac
      241 gagcacacaa cacccatcat tccagcgcaa cagacccata ttgctcagtt gccttctgca
      301 tcaggcgacg tctttatctg gatgggcgat ctgtggggat cgcgtccgga cggcatcaaa
      361 gggcacgatc tccagcactg gagcagccca atggttttcc gtcccgatgg caccattgag
      421 cctctgaaac gagaggaatc gtggagtttt gtgcttcgtt ctgctgccgt tggtcgttga
      481 gagttcatag gcacgggtca aggaagagtg caggagtcga actgcacctg tgctctgcca
      541 acctacgtga acagcgttcc ttgactgata gcactcaata gcctatatta caaaccatct
      601 ggaggagagt agaaccatga aaaagatatt tttggtagtg tgcttgtgcg ccgttgtaag
      661 tatgggttgc agtatggcgc agattcgcgc tttgtttggt gccatcgaga agccgaccat
      721 cgagtcaatt cgcccacgta tattagggct ggacttctcg ggtatcaatc ttgcttttga
      781 cgtggacttg aacaatccca attccttccc cctcaagact ccgaagttca agtacggctt
      841 ggacattcag ggaagcaact tcatgaagtc cgaaggaact gcggacattg atcttcccgc
      901 caaaggcgtg ggcactgtgg tgcttccagc gaagattaag tatctggaca tctggaagac
      961 gtacaagggg ctcaaagatg ccaaccaggt gagctacgat ctgaagggag cctttgtggt
     1021 ttctcctttg ggcaaagata tcgagttgcc catgagcaag agcggatcgt ttcccgtttt
     1081 gaagatccct aacttcagca agatttcact ggagccttcc gaggtcagtc tcactgagtc
     1141 gaaagtcaag gtcaaagcaa acgtcacaaa tccaaacgtt ttcgagatgg gtttggacaa
     1201 actcggatac gttctgaaaa tgggcgatgt ggattttgcc gacatcaagt ctactctgag
     1261 caagtctctg caggcagggg agaccggcca gattgaactg gatgcaaagg tcggtggact
     1321 ctctgttctg ggaaggattc tcgatggcgt ggatatgggc aaactcagcc ttacgcccga
     1381 gggaagtgta acaactcctt acggagagat tccactgcca aaatagattc aagttgatcc
     1441 gcctcaagcg tatcttgaat tccaaatcaa cgggaggtcg tctttgatgg agtcaattat
     1501 gggtttgaat tttggattgg gagcgcttcc aatgctctcc aacgcagaga cacgttccat
     1561 ctgcgcagaa aaccccaatg gggagagagg tgctggggcg aaggagattc ctgacgcggg
     1621 caacgccggc gcgatgctgg gtcagggctg gaaagtgcgt ccgtgtatca cactggaacc
     1681 tggaaccaca acgacactgg cggatatcaa gggtcccggc gtgattcaac atatttggat
     1741 cacggtggat ccaaaggcgt atcgtgactg cgttctgcgc ttctactggg acaaagagaa
     1801 gacgccatcc gtggaagtac ccctcggtga tttcttctgc aatggtcacg gcatccgtca
     1861 caaagttgac tctctcgccg ttgcggtcaa cccgagcggt ggcttcaact gctacttccc
     1921 gatgcccttt agaaagcacg ccaagatcac gattgagaat cagcgtcacg aagcgatctg
     1981 gggattcttc tatcagatca actacgcgtt gaccgaagtt ccaagggatg ccgcctactt
     2041 ccacgcacaa tggcgccgct ccgtgacgac acgtgagtat cccgaacatg tgctcttgga
     2101 tggaatcaaa ggacaggggc actacgtcgg aacttttctg gcgtggacgc agatgtccac
     2161 gggctggtgg ggcgaagggg agatcaagtt ctacctggat ggtgactcga agttcccgac
     2221 gatctgcgga acgggaaccg aggattactt cggcggagcc tggtgctttg gcgagaccta
     2281 ttccacggcc tttctcggct atccgctatg gcacaaagtt gagggacagg ttcccaaaca
     2341 tgacctttac cgatggcata ttctcgatcc gatacgcttt aagaagaaca tgaaagtgac
     2401 catccaagcg cttggctggt ggccaaatgg caagtatgag ccactgacag acgacatcgc
     2461 gtcaatcggc tactggtatc aggttgagcc acacgccacg ttcccccaat tcccggcagt
     2521 gcatcagcgc tggcctcgat agatgatgct cagacggtat tcatgaaggg cttcgaggtg
     2581 ttacagactc ggagcccttt tgtcagaggt ttgggagagg agaggttatt gtagcgattc
     2641 ccatcgctgc ttggagatat ttgcctgccg aaggattcgt gccagtagat ctcgaccaat
     2701 atctccaacg tgtggattgg ggatgctcag agtcaggtcg ccttggatca tgaaggcgtg
     2761 gtttccacca gagtatggac catcaaatcc acactggcgc agaaacttga caagatcacg
     2821 tcgggagact gggccgaatt tgggcactac gccgcggtct ccttcttcac agtcagttca
     2881 agtccgtcaa taaccggaat ggagagattc ttatggattc tgaaaagaag ccagtcttcc
     2941 agaacgcttg ccaattcttc tctacaggct tccagagtct gagcgttggc gtagactccg
     3001 ggacaaagag agatctcgcc gtaatagctg ccatcctctt caagcagctc gtagtgagcg
     3061 tgcttcattg aggcttcaag gtattttgag agcattcagt cagcctcctc tgaaaaagga
     3121 ctgttcacag acaggaaact actcttcgtc atgcgaacgt tcttcctgtt ttgactacta
     3181 tgaacaataa tgctcgcaac ctctgcagag atcaagctaa tctgctgaag aggtgaccgt
     3241 ccctgcaaga ccggttgaga aaagatggaa cccctgctag aaatactgat cgagatgatc
     3301 cttgtgaaca acgatctcat acatcatcct gagattcttc catcggagat gcggttcagt
     3361 gaccagatcg gagcggcaga ctattgagca cagccacgga atcatctgag tcttcaaatc
     3421 gcccgatgtc tgtcttggtt cataggagtg tccgtcagac tctgttccga atggcttttc
     3481 cgatgctggc gggaaccttc gccatgaacg cctataatct ggcggacacc tggtttgttt
     3541 ctcgacttgg aacgcttcct ctcgctgcga tgggattcac cttcccggtc atcatgctgt
     3601 tgacttgtct ggcaagtggc ttgggcacag gcgtgaccac gcttgtctcg cacgcaatcg
     3661 ggagacatga tcacgatgac gcggcacgga tcgtcactca cggcgtcata atgacggtca
     3721 gcatttctct gcttatctcc atcgcaggct atctgagtat tgacgcgatc tttgcgcgac
     3781 ttggcgctcg cggtgaggtt cgcatccttg tcgggcagta catgcgaacg tggtatctcg
     3841 gtgcgctcac gatgtcattg cccatgctgg gcaacgggat actcatttcc tcgggtgact
     3901 caaagagggc aagtcgtttg atgatcctcg gaacgggtct caatgtcatc ctcgatccga
     3961 tcatgatctt cggatacttc ggtttcccgg caatggg
//
LOCUS       DAOQUC010000181         3983 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_25523_length_3983_cov_2.797352, whole genome shotgun sequence.
ACCESSION   DAOQUC010000181 DAOQUC010000000
VERSION     DAOQUC010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3983)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3983)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3983
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25523_length_3983_cov_2.797352"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..803
                     /locus_tag="PKH07_04420"
     CDS             <1..803
                     /locus_tag="PKH07_04420"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HNT34223.1"
                     /translation="TESLTGRFFLHRCTHWSFSECRDAFEWSVEEWIYFGGYPGASAF
                     IENETFWKRYVVDSLIETVLARDVLHMVKVTKPALLRHLFALSATFPSQIVSYNKMLG
                     QLQEARNTTTLANYLRLLESAFLVSGLELFSRGTRRKRGSSPKLVLWNNALVNALSTH
                     DFESAIADSVWWGRLVENAVGAHLLNGLNSVEYSVTYWREGHHEVDFIIARGRDVWAV
                     EVKSGRSCKATGLDQFRQRYPGARMLLVGSQGVPLDEFFERQASDWLR"
     gene            complement(862..3375)
                     /locus_tag="PKH07_04425"
     CDS             complement(862..3375)
                     /locus_tag="PKH07_04425"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HNT34224.1"
                     /translation="MGGWCLVGFLTVFLTVANVLDARAADIPKNAASSKQDAGGVFGI
                     NGVGAFHQRHALQGDVLRRISNLKQLGIRWDRSDFWWHVLEPREGEFDWSFSDALLDL
                     YDQHDLQIFPILCYSSAWARGDGFAPANELEWKQFANFTFEAVNRYKDRIRYWEIWNE
                     PNILPFWRPQPDVEDYTRLLKEAYKAAKRADPNCIVVGMCTAGFDLKFIERAYQLGAR
                     DYFDVLSCHYYLTGEPEQAIKRDLQNLRHLMTRYGDSDKKVWITEMGVTSHPNRKHGV
                     SEQQQANLLVRNYLSNLATGLVEKIFWFCLVDWTDNPDDERWDSYLGLLRYDRSEKPS
                     FEACRNLATWLDGAEYVGDPRLDGKVHSWLWKKGEDLILIACTSGESVDLMLPTAHSS
                     LKHHGLFGETQTIDGLEDGNAHCTINETPACFVGISESLLLRTSFRFCPDSLTLQPEE
                     TVKAQLVFLNPFREKIKARLSIESPKGWKVRCPRSLTLAPQAERRVSVTLRAPSDSIA
                     RDHTIRTTLEIQGDELSEAASSSFPLPMEATLRAVVSDAVSLRLRPIIDQDQIVTTLE
                     VASHLTNPSMCDLSMRFPWKSEKTRKTVPAGEQIRWECRSSLAELRNMRITEPIVAGV
                     KIARKPHPIEKRFNIAVIPLVESSSWSRDPEKPTVSLRQVHALTGIGSESDFTSDVWL
                     AFSKQSLLVCARFHDCTPAQNPFDGEDIWKGDSLEIYLGFGGPEERPYFSPRDFQMLV
                     SPGIRGENAQIFSFPQKRKISEAQISVDLFDSGCEIKAEIPLSVFGEPGVESGQIIGL
                     DVAAHDYSPDRQPEMQALMWNGTGANWLTPRDWGIGVIR"
     gene            complement(3449..>3983)
                     /locus_tag="PKH07_04430"
     CDS             complement(3449..>3983)
                     /locus_tag="PKH07_04430"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNT34225.1"
                     /translation="QKRYADATRVLDKLLEIKPDEMNYFLKLGYLYDKQNLLEDAEQV
                     YLKVINSYPNKPEGYNHLGYMFAERNHRLDDAERLIRKALELSPDSGDIVDSLGWVLF
                     RKNMYPQAEAELLRALQLKGDDAVIIEHIGDVLLAQNRTQEAIERWQQALLLDAPEED
                     KERIRGKIEKAMSVLRP"
BASE COUNT          890 a         1067 c         1052 g          974 t
ORIGIN      
        1 ttacggagag tctaacggga cgattctttc ttcaccggtg tactcattgg agtttttccg
       61 aatgtcgtga cgcctttgag tggagtgtcg aagaatggat ctactttggt ggctatccgg
      121 gagcctctgc gttcatcgag aacgagacgt tctggaaaag gtatgtcgtg gattccttga
      181 tcgagaccgt tcttgcccga gatgttcttc atatggtaaa ggttacgaaa cccgctcttc
      241 ttcggcacct ctttgccctg tctgcaacct ttccaagcca gatcgtttcc tacaacaaga
      301 tgcttggtca actgcaagag gcgagaaata caaccacctt ggcaaactat ctgcgacttc
      361 ttgagtcagc atttctggtc agtggccttg aacttttttc tcgtggaacg aggcgcaaac
      421 gcgggagcag tcccaaactt gttctatgga ataacgcctt ggtgaatgcg ctctcgactc
      481 atgatttcga gagcgccatt gccgactcgg tatggtgggg aagacttgtg gaaaatgcgg
      541 ttggggcgca tctgctgaac ggcctcaact ctgtcgagta ctccgtcaca tactggcgag
      601 aggggcacca cgaagtggac ttcatcatcg cacgagggag agacgtctgg gcggttgaag
      661 tgaagagcgg tcgaagctgc aaagcaactg gtctggatca gtttagacaa cgctatcccg
      721 gtgcgagaat gcttctggta ggttcccaag gtgtgcccct cgatgaattc tttgagagac
      781 aagcctcaga ttggcttcgg tgagggctcc cagcccgaga aagatccacc atatcccagc
      841 tgcccataca ctctgtacat ctcatcggat aacgccgatg ccccagtctc gtggagtcag
      901 ccagtttgca cctgttccgt tccacatcaa cgcctgcatc tcgggctggc ggtctgggct
      961 gtaatcatgt gccgcgacat ccaaaccgat gatctgtccc gactcgacac ccggttctcc
     1021 gaataccgac agcggaatct ccgccttgat ttcgcagcct gagtcgaaca ggtcaaccga
     1081 tatctgtgct tcgctgattt tgcgtttctg tggaaaagag aatatctgcg cgttctcgcc
     1141 tcggatgcct ggtgacacaa gcatctggaa atcacgtggc gagaagtaag gtcgttcttc
     1201 agggccaccg aaaccgaggt agatttcgag cgagtcgccc ttccagatgt cctctccgtc
     1261 gaaaggattc tgtgcaggcg tacaatcgtg aaatcgcgcg cagaccagaa gactctgctt
     1321 cgagaatgcc agccacacat ccgaagtgaa atcactctca gagccgatgc cggtcaaggc
     1381 atgcacctgg cgaagagaga ctgttggctt ctcaggatct cgactccacg aggaactctc
     1441 aaccaaaggg atcaccgcaa tgttgaaccg cttttcgatc gggtgaggtt tgcgggcgat
     1501 cttcacccct gcgacaatag gttccgtgat tctcatgttg cgaagttcag caagggaact
     1561 ccgacactcc catctgatct gttcccccgc aggtacggtc ttccgggtct tctcgctctt
     1621 ccagggaaag cgcatactga gatcgcacat tgagggattt gtcagatggc tggcaacctc
     1681 caatgttgtg acgatctgat cctggtcaat gattggccgc agtcgcaggc ttaccgcatc
     1741 gctgacaacg gctctgagcg ttgcttccat cggcagggga aacgatgaag aagcggcctc
     1801 gcttaactca tcaccttgga tctccagtgt tgtgcgaatt gtgtggtcgc gggcgatcga
     1861 atcggatggt gcccgcaatg tgacgcttac tctgcgctca gcctgaggtg caagagtgag
     1921 acttcgggga caacgcacct tccagccttt gggagactcg atcgaaagcc gtgccttgat
     1981 cttctcgcgg aagggattga ggaagaccag ttgggctttc accgtctcct caggctgcag
     2041 tgtcaggctg tccggacaga accggaaaga cgttctgagc aagagactct cagaaatccc
     2101 aacaaagcaa gccggcgttt cattgatggt acagtgtgca ttcccgtctt cgaggccatc
     2161 gatcgtctgg gtctcaccaa aaagaccatg gtgtttgaga gaagagtgtg ctgtcgggag
     2221 catcagatca accgactcac cgcttgtgca tgcaatgaga atcaaatcct ctcccttttt
     2281 ccacagccag ctgtgaacct tgccatccag gcgtgggtct ccaacatact ccgccccgtc
     2341 cagccaggtg gccaggttcc tgcatgcttc aaaagacggc ttttccgatc ggtcgtagcg
     2401 aagcagtcca agataggaat cccagcgctc atcatcggga ttgtccgtcc agtccacgag
     2461 acaaaaccag aagattttct caacaagtcc ggtggcaaga ttcgacagat aattccgcac
     2521 cagcagattc gcctgctgtt gctccgaaac gccgtgtttg cggttcgggt gggaggtgac
     2581 acccatttcg gtgatccaga ccttcttgtc cgagtctcca tatcgggtca tcaagtggcg
     2641 aaggttctgc aagtcgcgct tgatcgcctg ttcaggttcc cccgtcagat agtaatggca
     2701 ggaaagcaca tcgaaatagt ccctcgcgcc cagttgatat gcccgttcga taaatttcag
     2761 atcaaatccg gcggtacaca tgccgacgac gatgcagttg gggtcggctc gcttggctgc
     2821 cttgtaggct tccttgagaa gtcgtgtgta gtcttccacg tctggttggg gtctccaaaa
     2881 tggcaagatg tttggttcgt tccagatttc ccaatagcgg atgcgatcct tgtagcggtt
     2941 gaccgcttca aaggtgaagt tggcaaactg tttccattcc agctcatttg ctggggcaaa
     3001 tccgtctccc cgtgcccaag cagaggagta gcaaaggatg gggaaaatct gaagatcgtg
     3061 ctggtcgtac agatcgagca gtgcgtcgga aaaactccag tcgaactctc cctcgcgagg
     3121 ttccaaaaca tgccaccaga aatcgcttcg atcccagcgg atacccagtt gtttcagatt
     3181 actgattcgg cgcagaacat ccccctggag ggcatggcgc tgatgaaatg ctccaacgcc
     3241 attgatgcca aagacaccgc ccgcatcttg ttttgacgat gctgcattct ttggaatatc
     3301 ggccgctctt gcatcaagga cattggcgac agtcaggaag accgtcaaaa agccgaccaa
     3361 acaccaacca cccattttcg tctcctgcgc ttcagcgtat tggaattctt cttgcagagc
     3421 atcccgaggg actcatcgcg cattgcatct agggacgcag aacggacatg gccttttcaa
     3481 ttttccctcg aatgcgttcc ttgtcttctt ctggtgcatc caggagcaag gcctgctgcc
     3541 agcgttcaat ggcttcctga gttctgttct gtgccagaag tacgtcgcca atgtgctcga
     3601 taatcacagc gtcgtcgccc ttcagttgaa gagcacggag cagttccgct tccgcctggg
     3661 gatacatatt ctttctgaag agaacccaac ccaaactgtc cacaatatcc ccgctgtcgg
     3721 gtgagagttc cagcgctttt cggataagcc gctccgcatc atccagacga tgatttcgtt
     3781 cggcaaacat gtagcccagg tgattgtagc cttcgggttt gttcggatag gagttgatga
     3841 ctttgaggta gacctgctcg gcatcttcaa ggagattttg tttgtcatac aggtagccaa
     3901 gtttcagaaa gtagttcatc tcatcgggtt tgatctcaag aagcttgtcg agaactcttg
     3961 tcgcgtctgc atagcgcttc tgg
//
LOCUS       DAOQUC010000182         3968 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_25670_length_3968_cov_3.149502, whole genome shotgun sequence.
ACCESSION   DAOQUC010000182 DAOQUC010000000
VERSION     DAOQUC010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3968)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3968)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3968
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25670_length_3968_cov_3.149502"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            76..>3968
                     /locus_tag="PKH07_04435"
     CDS             76..>3968
                     /locus_tag="PKH07_04435"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HNT34226.1"
                     /translation="MNYVTGATAPTNTPTPVAYAAHWTAQSYADTMTSGSTAVCWVEY
                     MNDGTAVWGHAKTRLGTTQPRDRSSIFYTAGNWVGANRPTEVDQSAVSKGQVGRFTFI
                     MTAPAVGTSTTYTEYWGLVEEGVTWFGPADNAVWFRITVNPSGPTNTPTNTYTNTHTP
                     TRTPTPFLPSQLIIDNPDPGWSDDGFPWATATGASDKYGADYRYTSTNFGVRTASWSH
                     AINGGGTYKVYAWWPAGANRSNNTPYVVHTRTGDTMVWVNQEINGGTWNLLGSFAFSS
                     LLSVSISTQGAETGDGQIVMADAVMFSLEGGMQTPTNTNVIPTSTPTNTPTRTFTNSP
                     TATPTGVITSTPTRTPTQTPTHTSAPVSLIVDNTDPGYADVGSDSNWFTSSSATNKYG
                     VDYRYNTSGSGLDSATWTVVIPQAGNYEVAIWYPNGSNRANNAPFQVYHADGSSLFYV
                     DQQYSGGQWNKLGWFSFSTGSYSVVLNDDAQEGQIVVADAVRWALVQDTPTPSNTPVT
                     TPEGRAVWIDVFDMDSPSKIETAISKAAANNMNTVICQIRYRGDALYFPNRTDSTYPN
                     SEPRSERLSSQSPGFDPLQTAITLGHNAGLQVHAWVATYPAWSSAVPPTDPDHVFNAH
                     PEWITRNNSGTVMQVTDAEGAYLDPGRLDVQEYLGNVFKDIIINYDMDGFHLDYIRYP
                     TSSFDRSIDWGFNTVATDRYSEQTGQTADPTSATWQNWKRDQVTSLVEDIWDFILANK
                     PAIAFSCAVLGDRSTAEGWAFQHWSHWALQPILDLVYPMCYSTETSTVTTQAADAFAN
                     TGTRNVFIGLRSYGAGSYAASSLVEKVNQVRALPGFEPGKDGFAFFDSSGLQESGDVF
                     YSALKADPLASWRSMPPIPARPLPTPTATFPATVTPTQTPSRTPTETQSHTPTNTVIT
                     TPTDTPTQTPSDTPTFTITNSPIHTATFTHTPTNSPTNTPAVVEVTINTTKDTHVRDY
                     NDTYKTYNYGVFNRLLIGYYNGWGYYHQRALVEFSVASIPSNVTILDARIGLYQDVGA
                     SHASSDQPHTVHLYRVTSTWAEGTVNGAAGSVCWNDQPTINTTSLGSLNIEGVAAGTW
                     REWTGASVVNLVQGWVNGSITNRGVMFRNSTTNEDDDGAHKFYSDDQGTNPPRLWVRY
                     QLLPTPTPTNTPTRTPTPTVTPTPTSFTTHEAVINATKDSHIRDYNDDYKSRNYGLYN
                     RLLIGYYSGWGYAHQRAMVEFDISSIPSNATVLESNVSLYQDVGAAHASSDQSHDVHL
                     YRVTTAWQEGACNGTAGTVCWNDQPTINTTSLGSLT"
BASE COUNT          965 a         1198 c          993 g          812 t
ORIGIN      
        1 gcctcggctg ggacaacctc gccatctggt tccgcatcaa ccagctgtcc cggaccattg
       61 ccatggacga cttatatgaa ctacgtcacc ggcgctaccg ctccaaccaa cacgccgact
      121 cctgtagcct atgcggctca ttggactgcg cagtcctacg cggatacgat gacctcaggg
      181 tcaacagccg tgtgctgggt cgaatacatg aatgacggta ccgcggtttg gggacatgcg
      241 aaaacccgac tcggaacgac acaaccgaga gaccgttcca gcattttcta cacggcgggc
      301 aactgggttg gtgccaaccg cccgactgaa gtggatcagt ccgctgtatc caaaggtcaa
      361 gtgggacgat ttacgttcat catgaccgcg cctgcggtcg gcacttccac tacctatacc
      421 gagtattggg gactggtcga agaaggggtg acgtggtttg gtcctgcgga caatgcggtc
      481 tggttccgga tcaccgtcaa cccgtcggga ccgaccaaca cgccgacaaa cacatatacc
      541 aatacccaca ctccaactcg aacgccaaca ccgttcttgc ccagccaact catcattgac
      601 aatcccgatc ccggttggag tgatgacgga tttccctggg caacggcgac aggtgcctcg
      661 gacaaatacg gtgcggacta ccgttacaca agtaccaact ttggtgtgcg gacagcatcc
      721 tggtcacatg ccatcaacgg tgggggtaca tacaaggtct atgcctggtg gccggcggga
      781 gccaaccgct cgaacaatac tccctatgtt gttcatacga gaacgggcga caccatggta
      841 tgggtgaatc aagagatcaa cggtggaaca tggaaccttc tgggttcatt tgctttctct
      901 tcgctgctaa gcgtttcgat ttccacgcaa ggagcagaaa ctggcgacgg tcaaattgtt
      961 atggccgatg cagtgatgtt ctctctggaa ggtggaatgc agacacctac caacaccaat
     1021 gttatcccga ccagtacgcc caccaacaca ccaacacgca cattcaccaa ctcgccgaca
     1081 gctacaccca cgggtgtgat cacttccaca cccacacgga cgccaaccca gacaccaacg
     1141 catacttccg caccggtcag cctcatcgtg gacaacaccg atccaggcta cgcggacgtg
     1201 ggttcagatt ccaactggtt cacgagttct tccgcgacca acaagtatgg agtggactac
     1261 cggtacaaca ccagcgggtc gggactcgat tcagcaactt ggactgttgt gataccgcaa
     1321 gcgggtaact atgaagtcgc gatctggtat cccaatggaa gcaaccgagc caacaacgct
     1381 ccgtttcagg tttatcatgc tgatggttcc agtctcttct atgttgatca gcagtactca
     1441 ggtggtcagt ggaacaaact cggctggttc agctttagca cgggcagcta ttccgttgtg
     1501 ctcaacgatg acgcacagga aggtcaaatc gttgtggcag acgctgtccg atgggctttg
     1561 gttcaagaca cgcctacgcc cagcaatact cctgtcacca cgccggaagg acgtgccgtc
     1621 tggatagatg ttttcgatat ggattctcca tcgaagatcg agacggcgat cagcaaagcg
     1681 gctgccaaca acatgaacac ggtgatctgt cagattcgct atcgaggtga tgctctgtac
     1741 tttcccaaca gaacggattc cacctatccc aactcggaac cccgttcgga gaggctttcc
     1801 agtcagtcac ccggttttga cccactccag acggcgataa cactggggca caacgctggg
     1861 cttcaagttc acgcctgggt ggcaacctat cccgcttggt caagtgccgt cccgccgact
     1921 gatcctgatc acgtcttcaa cgcgcacccg gagtggatca cacgcaacaa cagtggcacc
     1981 gtgatgcagg tgaccgatgc tgaaggggct tatcttgatc ccggtcgtct tgatgttcag
     2041 gaatatctgg gaaacgtctt caaagacatt atcatcaact acgatatgga tggtttccat
     2101 ctggattaca ttcgctatcc cacaagcagt tttgaccgct ccatagactg gggcttcaac
     2161 acagtcgcga cagaccgata ctccgaacaa acaggacaga ctgccgatcc gaccagtgcc
     2221 acttggcaga actggaagcg agatcaggtt acttctctgg ttgaagatat ctgggatttc
     2281 atcctggcga acaaacccgc gattgccttc agttgtgcgg tgcttggcga tcggagcact
     2341 gccgagggct gggcgttcca gcactggagc cactgggcac tgcaaccgat tctggatctg
     2401 gtctatccca tgtgttacag cacagagacc agcaccgtga ccacgcaggc tgccgatgct
     2461 tttgcgaaca cgggtacgag aaatgtgttc atcggtttga gatcctatgg cgcgggttcc
     2521 tatgccgcca gttcactggt tgagaaggtc aaccaggttc gtgcgttgcc cggatttgag
     2581 ccgggcaaag acgggtttgc gtttttcgac agctccggcc tgcaggaatc aggcgacgtt
     2641 ttctatagtg cactcaaagc agatccgctc gcttcatggc gctcaatgcc tccgattcct
     2701 gcccggccac ttcccacgcc aactgcaact tttccagcaa cggtcacgcc aactcagaca
     2761 ccttcgcgca cgccaacgga aacgcaaagc cacacaccga ccaacacggt catcaccact
     2821 ccgacggata ctccaactca gaccccgagc gacaccccca ctttcaccat aactaactca
     2881 ccaattcaca cggcaacctt cacgcatact cccaccaact cccctacaaa tacccctgct
     2941 gtggtggaag tgacgatcaa caccaccaaa gacacacacg tccgagacta taatgacacc
     3001 tacaagactt acaactacgg cgttttcaat cgcctgttga ttggttatta caacggctgg
     3061 gggtattacc atcagcgtgc cttggtcgaa ttctcggttg cctccattcc gagcaatgtc
     3121 accatcctgg atgcccgaat cgggctctat caggatgtcg gcgccagtca tgccagtagc
     3181 gatcagcctc acacggttca tctttaccgt gtcacaagca cctgggcgga agggacagtc
     3241 aacggtgccg ccggttcggt gtgctggaac gaccagccga ccatcaacac caccagcctc
     3301 ggctccctga acattgaagg agtcgcagca ggaacttggc gggaatggac gggagcgtcc
     3361 gttgtgaatc tggttcaggg ctgggtcaac ggaagcatca ccaaccgcgg tgtgatgttc
     3421 cgaaacagca ccaccaacga agacgacgac ggcgcgcata aattctattc ggatgaccag
     3481 ggaaccaatc ccccgcgatt gtgggttcga tatcaacttc tgccaacacc gacgcccacg
     3541 aacacaccga cgagaacacc aacaccaacg gtcacgccca cgcccacctc attcacaact
     3601 catgaagccg tgatcaacgc caccaaagac agccatattc gcgactacaa cgacgactac
     3661 aagtcccgca actatggtct ctacaacagg ctcctgatcg gatactacag cggttggggc
     3721 tatgcccatc agcgggcaat ggtcgagttt gatatttcca gcattccatc gaatgcgact
     3781 gtcctggaga gcaatgtatc actctaccag gatgtgggtg cggcacatgc cagcagcgat
     3841 cagtcccacg acgtgcatct gtaccgagtt accacagcct ggcaggaagg tgcctgcaac
     3901 gggactgccg gaacggtctg ctggaacgat cagccaacca tcaacaccac aagccttggc
     3961 tctctcac
//
LOCUS       DAOQUC010000183         3958 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_25764_length_3958_cov_3.037407, whole genome shotgun sequence.
ACCESSION   DAOQUC010000183 DAOQUC010000000
VERSION     DAOQUC010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3958)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3958)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3958
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25764_length_3958_cov_3.037407"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..633
                     /locus_tag="PKH07_04440"
     CDS             <1..633
                     /locus_tag="PKH07_04440"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HNT34227.1"
                     /translation="LGVKVKEVPDEVREHSSIPGEGGALVEEVIKDGPAAEAGLQEQD
                     IILELNGKPVAGSRELVQMVGQIPVGEKAKITIYRPKDSKKMTLSMVTTERPVDIDSV
                     YVAPATKEALGITVQNLTEDLGKRLGYEGDQGVLVTSVDAKSPAAGQLQEGDLIIEVE
                     WNKVKNTEAFWDAVKKVEETQKDKGVLLRVRRKANTFLVVIKFDSEAKNE"
     gene            740..1246
                     /locus_tag="PKH07_04445"
     CDS             740..1246
                     /locus_tag="PKH07_04445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34228.1"
                     /translation="MKTLRILGLLILGFVVVGQVFAGTVELQDGKQRQGQVIVRENVV
                     TINTSDNKTFQFYLNQVKKITATEEKKHLIATDVTLKSEANDDAAAGQTLSKGMEVEE
                     LEQKDGWLKVKAVRENEVGWVKPTNLVKELVFEGVAPEVAEAATESASPKQPSVAAEQ
                     PKDATPSE"
     gene            1341..2411
                     /gene="tsaD"
                     /locus_tag="PKH07_04450"
     CDS             1341..2411
                     /gene="tsaD"
                     /locus_tag="PKH07_04450"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HNT34229.1"
                     /translation="MLTILGFETSFDDTAAAVVRNGTEILSNVMASQVDLFKDFGGVV
                     PEIASRAHIQMLLPVMHRALSKAELTLGDMDALAVTYGPGLVGSLLVGVSVAKGLSYA
                     LGLSLYAINHVEAHIYAPLMTNPELEFPHICLSAAGGHTSLYLVKSLGDYQMLGGTRD
                     DAAGEAFDKVSVAMGKGCPGGRAIEQLAAQSDQEGPSFPRPMMKSGDYDFSFSGLKTA
                     VVRYLETPEGRQSSLEATAAAFQSAAVDLMVSKTLRAARNSGARHISLTGGVACNGYL
                     YEQLKARCERKHLRVFRPPASLCTDNAAMVAGLAFHKHRLDSPAGLELNADPGLGLGK
                     TNPTLLVDTPRQGSRKTRPRTP"
     gene            2408..3394
                     /gene="dusB"
                     /locus_tag="PKH07_04455"
     CDS             2408..3394
                     /gene="dusB"
                     /locus_tag="PKH07_04455"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HNT34230.1"
                     /translation="MNAPTQEWLFAKGRPALLSAPLEGISDVFWRSLAFRFGCSAAYT
                     EMLSAEALLRRIRQSWNKLEEVPKQFPVIVQIFGAEPASLADAAALCQDAGAAAVDIN
                     MGCPVPKIVSHGKGAALMREPALVRDILRAVRSRIAIALWIKIRLGWDDKSRNYLEIG
                     RLAESEGVDAIALHGRTRTQKFTGLSDWEAVGSLKSQVGIPVFGSGDVDSGQKALEHF
                     KKTDCDGILIGRGALGNPWIFAESEATLHGRAFSAASCEDRCAVMIEHVQWMVERFGL
                     EKGLRDARKHIGWYVRGFPGNKQFREWFYQCTCVESAVEEIERFLKSKPPSN"
     gene            3583..>3958
                     /locus_tag="PKH07_04460"
     CDS             3583..>3958
                     /locus_tag="PKH07_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004075071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HNT34231.1"
                     /translation="MDFLKKIFGGKHTAEKPDFSKDTCYRTLGISWSASQQEIKAAYF
                     GKVKEYSPEIYPEQFMAIRKAYEVLKEPDSKAELDVTCLAEPSLKIQFSDVPEASSSQ
                     VKINQAIAEAEKAAESGDSIARA"
BASE COUNT          932 a          956 c         1192 g          878 t
ORIGIN      
        1 cttggtgtga aggtgaagga agttcccgac gaggttcgtg agcactcaag cattccgggt
       61 gagggcggtg ctcttgttga agaggtgatc aaggatggcc ctgcggccga agcaggtctt
      121 caggaacagg acatcatcct cgagctcaat ggcaagcctg tggcgggctc ccgggaattg
      181 gtgcagatgg tggggcaaat acccgttgga gagaaagcta agataaccat ctaccgtcca
      241 aaagactcca agaagatgac cctgtccatg gtgacaacag agcgtccggt ggacattgat
      301 tccgtctacg ttgcgccagc gacgaaggaa gccttgggga ttactgttca gaatctgaca
      361 gaagatctgg gcaagcggct gggatatgag ggagaccagg gtgttctcgt gacatccgtg
      421 gatgccaaga gccctgcggc cggtcagctc caggagggag acctgattat tgaggttgag
      481 tggaacaagg tcaagaatac cgaagcgttc tgggatgcag tcaagaaagt ggaggaaact
      541 cagaaagaca agggtgttct gcttcgggtt cgccgcaaag ccaacacgtt tctggttgtg
      601 ataaagtttg attccgaggc gaagaacgag taagattccc cctgctgttg gatactgctg
      661 gtggagagtc tcatccgaaa ggacagactc tccaccgcag gtcagcgcat gcaggcattt
      721 ggagacagga ggaaggttca tgaagacgtt gaggattttg ggtctgctca ttctgggttt
      781 tgttgttgtt ggtcaggtgt ttgccggaac ggtcgagctt caggacggca agcagcgtca
      841 gggacaggtc attgttcggg aaaacgtcgt gacgattaac acgtcagaca acaagacctt
      901 tcagttctat ctcaatcagg tgaagaagat cactgccacg gaagaaaaga agcacctgat
      961 tgccaccgat gtgacgctga agtctgaagc caacgatgac gccgctgcgg gacagacgct
     1021 ctccaaggga atggaagtcg aggaattgga gcaaaaggac ggttggctga aagtgaaagc
     1081 tgttcgtgag aacgaggtcg gctgggtgaa accaaccaat ctggtcaaag aactggtctt
     1141 tgagggagtc gcaccagaag tcgcagaagc agccacggaa tccgcttcac cgaagcaacc
     1201 gtcagttgcc gccgagcagc caaaagacgc cactccgagt gaatagagcg aacgctcaat
     1261 ccatctggat gtccaaacag agaaacacca gtcgcttggc atctgctttt gccaggcgac
     1321 tgttcattag aggctgatcg ttgttgacga ttcttggttt cgagacatct tttgacgata
     1381 cggctgctgc cgttgtgcgc aacggcacgg agattctgtc caacgtcatg gcaagccagg
     1441 tggacctgtt caaggacttt ggtggtgttg ttccggagat cgcttcgcgg gcgcacattc
     1501 agatgttgct tccggtcatg cacagagcgc tttcgaaagc agaactcacg cttggggata
     1561 tggacgcttt ggcagtgacc tacggccccg ggctggttgg atcacttctg gtgggagttt
     1621 ctgtggccaa ggggctttcc tacgcactcg ggttgtcact ttacgctatc aatcatgtcg
     1681 aagcacatat ctacgctccg ttgatgacaa atcccgaact tgagttccct catatctgcc
     1741 tgtcggcggc gggaggacat acatctctgt atctggtcaa gagtttgggc gactatcaga
     1801 tgctcggagg cacccgggat gacgcggcgg gagaggcttt tgacaaagtc agcgttgcga
     1861 tgggaaaagg ttgtcccgga ggaagagcca ttgagcaact ggctgctcag agcgatcaag
     1921 agggtccttc ttttccacgt ccaatgatga aatcaggcga ctacgatttc agcttcagtg
     1981 gactgaaaac ggctgttgtt cgataccttg agactcccga aggtcgtcag agttcgctgg
     2041 aggcaacggc ggcggcgttt cagagtgcgg ctgtggatct gatggtctcc aaaacactcc
     2101 gcgcggctcg aaacagtggt gcccgccata tctctctgac cggcggtgtt gcctgcaacg
     2161 gataccttta cgagcaactg aaggctcgtt gtgagcgcaa gcacctgcgt gtgtttcggc
     2221 ctccggcgag tctctgcaca gacaatgccg ccatggtggc tggactggcc tttcacaagc
     2281 atcgtctcga cagtcccgca ggacttgaac tcaatgccga tccaggattg ggactgggca
     2341 agaccaatcc gactctcctg gtagacacgc cacgtcaggg aagcaggaag acccgcccga
     2401 gaacgccatg aatgctccga cacaagagtg gctttttgcc aaagggaggc cggcgctgct
     2461 gtcagcgcct ctggaaggaa tctccgatgt cttctggaga agtcttgcct ttcggtttgg
     2521 gtgcagtgcc gcctacacgg aaatgctcag tgccgaagca ctgctgagac gaattcgaca
     2581 gtcgtggaat aagcttgaag aggttcccaa acagtttcca gtgatcgttc agatatttgg
     2641 agccgagccg gcgagtcttg cggatgccgc ggcactttgt caggatgcag gagccgccgc
     2701 ggtggatatt aacatgggtt gtccagttcc gaagatagtc tcgcacggca agggagcagc
     2761 gctcatgcgc gagcccgctc tggtccgaga catcctgcgg gcagtcagga gtcgcattgc
     2821 cattgccctg tggatcaaga tacgtctggg ttgggacgac aagagccgga attacctgga
     2881 gatcggaaga cttgctgagt cggaaggcgt ggacgctatt gccctgcatg gtcgcacacg
     2941 cacacagaaa ttcactggac tgtctgactg ggaggctgtc ggatctctga agtctcaggt
     3001 tggcatacct gtgtttggaa gtggtgatgt tgacagcggc cagaaggcgc tcgaacattt
     3061 taagaagacc gactgcgatg gcattctcat cggacgtggc gctctgggca atccgtggat
     3121 ttttgcggaa tctgaagcca cattgcatgg aagagcgttt tccgcggcat cctgtgaaga
     3181 ccgatgtgcc gttatgatcg agcatgtgca atggatggtt gagcgtttcg gtctggagaa
     3241 gggtttgcgg gatgcgcgaa agcacattgg ctggtatgtt cgaggctttc ccggaaacaa
     3301 gcagtttcgg gagtggttct accaatgtac ttgcgtggaa tcagccgttg aggagataga
     3361 acgttttcta aagagcaaac cgccttctaa ctagccagtc agaagaggtt gtgctaagtt
     3421 cgctgtcgtt aaataacgtc aagggaatcg agcatcatcg tgctcgacct gaaccgaggg
     3481 actaagtttc ggggtgccct ctcatgttcc tgcgctacgg atgtgcctcg gtgacattgc
     3541 tgcagttgaa ccttaaacgt ttgcgaatag ggatgtcttc aaatggactt tctcaagaaa
     3601 atcttcggtg gcaagcacac tgcggagaaa cctgattttt ccaaggacac ctgctaccga
     3661 acactgggga tttcctggtc ggcctcacag caggagatca aggccgccta tttcggaaaa
     3721 gtcaaagagt attcacccga gatctatcct gagcagttta tggccatccg caaggcctat
     3781 gaagtgctca aagagcctga cagtaaagcc gaactggatg tcacctgtct ggcagagccc
     3841 tcactgaaaa tccagttcag cgatgtacct gaagcgtcct cctcgcaggt gaagatcaac
     3901 caggccattg ccgaagccga gaaagcggcc gagtccggtg attccatcgc gcgggctc
//
LOCUS       DAOQUC010000184         3945 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_25862_length_3945_cov_4.075064, whole genome shotgun sequence.
ACCESSION   DAOQUC010000184 DAOQUC010000000
VERSION     DAOQUC010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3945)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3945)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3945
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25862_length_3945_cov_4.075064"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            298..753
                     /locus_tag="PKH07_04465"
     CDS             298..753
                     /locus_tag="PKH07_04465"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HNT34232.1"
                     /translation="MAVVQWRPRQEWFPFPGLFSLREEMDRFFRDAVGEAATEGAVGF
                     IPPLDLAESEDSFTVQLDLPGVKQEDIEVSILGRTLTVKGEKTKESEIKEQNYHRTER
                     FVGSFRRAITLPSGVDAEKVKATYKAGVLELEIPKKEEAKPKQITVQTK"
     gene            complement(847..2604)
                     /locus_tag="PKH07_04470"
     CDS             complement(847..2604)
                     /locus_tag="PKH07_04470"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNT34233.1"
                     /translation="MTGKFWRFSFSVFGYLSAILMLMPVLHAFGVQDTDVVARFKDGQ
                     ITVGDVKSFWEEQGESNLVVRSATDENRIQVVAEELALQEILLDHAKQKGFEDDESVQ
                     QWTENLLERLLIDELLKKEVGSKIQIEDSSIQAYYEANPDLSLIPEKFSIRHIFFDTT
                     DVTATLINKRHQDALDVRSRIVQGEDFLAMALQYSQTEGEKGAVVTLPVGQISPLLER
                     AALSMEIGQISPVMETKYGFQLIQLVGQEPASKKSLEEVRGVIVSKMRQEQEETYKEQ
                     LRDKIMETRKSLKAKYDLEDLDSLPRTSDKVICEIPGVLTVTAAQLKDVLSRMAPASA
                     PVCDATTARDIIENGFIFQTLSIRYAKELGLDQSLEIQDRVTKEKKRLLISKSRRDLV
                     ESLATETTPKPEDIRNYYDTNSNEFMTEATYFYETLSVPIAPLKITEPTVSDQHVAIR
                     RAKRRAEELLGRFSKGLTFEQAAQESEDLVYTPESEPTVIRSGEAADALDQTLASLSA
                     GEVCKEPLLQMNKFLLVRVLRIEEPHSQPFEEVEQRVKARLEQEIRSRTDSGLKQQLL
                     EESGFSLDKKIAAQVKLDQ"
     gene            complement(2692..>3945)
                     /locus_tag="PKH07_04475"
     CDS             complement(2692..>3945)
                     /locus_tag="PKH07_04475"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HNT34234.1"
                     /translation="KVILNNGGVLEGIILKETEEEVVVMSRVGELTLSRSTIAEIQRG
                     NSYQVYLDRAESFIRQKKFLSARENLTLAERVGCPKDLLKKHQTLLEHASRNETERQM
                     NEQLATADALRAKGRLQDAIDTLNRLPTEQKNDPRVRRALTQMLCGSAYEHIDHFRFN
                     QAVRNMIQARDLGAPKAELHFLMGLLKQSEGRMALAQYEFGEALQADPDVYNRFPGSI
                     KSPLFPVPIEARAEVAMISAASLDLNISELDIGLDRQIALKTHPLDQKTTKTELLIPP
                     GTSADKEEILRHIHHFADIYGVDPCLVEAVAKVESALNPKAVSPAGAKGVMQLMPLTA
                     KDMGVKDIFDPKQNIEAGAKYLGLMMRKFQNTELALAAYNAGPSTVEFYGKIPPYPET
                     KRFVPLVIKERDRIRSSRDQLVQSK"
BASE COUNT          898 a         1064 c         1013 g          970 t
ORIGIN      
        1 cactgaagca acctcagcaa cagcctcgcc aaactgacac agacgctatc gcctcgttgg
       61 ttcattttga cacactcaat agtcagtacc gagatctgtc tctccttttg gcagagagaa
      121 acatcagggc atgtcctacg tactctattg tctttttttg acttagagac agatgccatg
      181 gaaatcaaca gaattggcat gctcgctgct aaccgaagaa agcagaacaa gaaacttgga
      241 gtcaagatga ggaaggctcc aacacaaaag gaaaaatttg ggaaaggaga atgcatcatg
      301 gcagtcgttc aatggagacc gagacaagag tggttcccgt ttcccggact tttctcactt
      361 cgtgaagaga tggaccggtt ctttcgggac gccgtgggag aggcagcaac agaaggggca
      421 gtcgggttta tcccgccctt ggatctggcg gagagtgagg acagtttcac cgtccagctg
      481 gatttgcccg gtgtgaagca ggaggacatt gaggtttcca tccttggcag aacgctgact
      541 gtcaagggtg agaagacgaa ggaatccgag atcaaagagc agaactacca tcgtacggag
      601 cggtttgttg ggagtttccg ccgtgcgatc acactgccct cgggtgtgga tgccgaaaag
      661 gtgaaagcca cctataaggc gggagttctg gaactggaga ttccgaaaaa ggaagaagcc
      721 aaaccaaaac agattaccgt ccagaccaag tagtcaggtt gcgggctatg cccgcttttg
      781 aggactgtgg cttctgacag aaagaatgcc aggcggttgt ctggggaagg gatcgcaaat
      841 cgcggcttac tggtctagtt tgacctgagc cgcgattttc ttgtccagtg aaaaaccact
      901 ttcctcaagg agttgctgtt tcaatcctga atccgttctg cttctgattt cctgctccag
      961 ccgagccttg acacgttgct caacttcctc gaagggctgg ctgtggggtt cttcaattcg
     1021 cagcacacgt accagcagga acttattcat ctgaagcaac ggttccttgc agacttcccc
     1081 tgccgacagc gaggcaagcg tttgatccaa ggcatctgcc gcctcaccgg aacgaattac
     1141 cgttggttca ctttcaggtg tgtagacaag atcctcggac tcctgtgccg cttgctcgaa
     1201 ggtaagtccc ttgctgaagc gacccagaag ctcctcagcg cggcgttttg cccttctgat
     1261 agcgacatgc tgatcggaca cagtcggttc tgtgatcttc aagggtgcga tcggcacact
     1321 caatgtttca tagaaatacg tggcttctgt catgaactca ttggaattgg tgtcatagta
     1381 atttcggata tcctcgggct tgggcgtcgt ctcagtcgca aggctctcga ccaagtcgcg
     1441 ccgagatttg ctgatcagaa gccgtttctt ctccttggtc acacgatcct gtatctccag
     1501 agactgatcc agccccaact cctttgcata tcgaatgctc agagtctgga aaatgaaacc
     1561 attctcaatg atgtcccttg ccgtcgtggc gtcacacacc ggcgctgaag caggagccat
     1621 ccgactcaga acatccttca actgagcggc cgtgacagtc aacactccgg gtatctcgca
     1681 gatcaccttg tctgatgttc gaggaaggct atcgaggtct tcgaggtcgt actttgcttt
     1741 cagcgactta cgggtttcca tgatcttgtc acggagttgt tctttgtacg tttcttcttg
     1801 ttcttgtctc atcttggaga caatcacacc ccgaacctcc tcaagggact tcttggaagc
     1861 cggttcctgt cccaccaact ggatcaactg aaaaccgtat tttgtttcca tgaccggact
     1921 gatttggccg atctccatgc tcagggctgc cctctcaagc agggggctga tctgaccgac
     1981 aggcagtgtg acaacggctc ccttttctcc ctcggtctgg gaatactgga gcgccatggc
     2041 cagaaaatcc tctccctgca cgatgcgtga gcgcacatcg agcgcgtcct gatgtcgttt
     2101 gtttatcagg gtggccgtca catccgtggt gtcaaagaat atgtgcctga tcgaaaactt
     2161 ttccggaatc agtgacaagt ccggattggc ctcgtaatag gcctggatgg acgaatcttc
     2221 gatctggatt ttggagccaa cttctttctt gagaagttcg tcaatgagaa gccgctcaag
     2281 caggttctcc gtccattgtt gaacactctc atcgtcctcg aagccctttt gtttggcgtg
     2341 atcgagcaga atctcctgca aggccagctc ttcagccacc acctgaatcc gattctcatc
     2401 agtggcagag cgaaccacca aattggactc accttgttct tcccaaaagg atttcacatc
     2461 tccgacggtg atctgtccgt ctttgaaacg ggcaacgaca tcagtgtcct ggacaccaaa
     2521 tgcatggagc accggcatca gcatcaagat ggctgacaag tacccgaaga ccgaaaatga
     2581 aaaacgccag aactttcctg tcatgactct ctccatcctt ttcgactcac tgatcgagca
     2641 ttgcagtgag atccacttcg aggggatccg actccatccc tctgcggcac tttatttgga
     2701 ctgtaccagt tgatccctgg aacttctgat tcgatcgcgt tctttgatca ccaacggaac
     2761 gaagcgtttg gtctcaggat acggcggaat cttgccatag aattcaaccg tactcgggcc
     2821 ggcattgtag gcggctagag ccaactccgt gttctgaaac ttcctcatca tgagaccaag
     2881 atacttggct cccgcctcaa tgttctgctt ggggtcgaaa atatctttga cgcccatgtc
     2941 tttggccgtg aggggcatca gctgcatcac acccttggca cccgctgggg agacggcttt
     3001 ggggttcaaa gcactctcaa cttttgccac tgcctcgacc agacaaggat ccacaccgta
     3061 gatatcagca aagtgatgaa tgtggcgcag aatctcttcc ttgtccgcac tggtgcctgg
     3121 gggaatgagc aactcggtct tggtggtttt ctgatcaagc ggatgcgtct tgagggcgat
     3181 ttgtcggtca agtccgatgt ccagctccga tatgttgaga tccaacgacg cagcggagat
     3241 catggccacc tcagcacgtg cctcgatggg aacagggaac aaaggcgact tgatgctgcc
     3301 cggaaatcga ttgtagacat ccggatccgc ctgcaaagct tctccaaact catactgggc
     3361 aagcgccatg cgaccttcgc tctgcttcaa cagccccatc agaaagtgca gttcggcctt
     3421 cggtgctccc agatcgcgcg cctggatcat attccgaacc gcctggttga aacgaaaatg
     3481 gtcaatgtgc tcataggccg atccgcacaa catctgcgtc aatgcccgac gaactctcgg
     3541 atcgttcttc tgctcggtgg gcagtcggtt gagcgtgtca atggcgtcct ggagtcgtcc
     3601 cttggcacgg agagcatcgg cagtcgccaa ttgctcattc atctgcctct cggtctcatt
     3661 gcgcgaggca tgttcgagca aggtctgatg cttcttgagc agatccttgg gacagcccac
     3721 tcgctcggca agggtgagat tctcacgagc gctcaggaac ttcttctggc ggatgaaact
     3781 ttcggctcga tcgagataga cctgatagga gttgccgcgc tgaatctccg cgatggtgct
     3841 tcgggacagg gtcaactccc cgacccgaga catgacaacc acttcctctt ctgtctcttt
     3901 gaggatgatc ccctcaagaa ccccgccgtt gttcagaatg acttt
//
LOCUS       DAOQUC010000185         3942 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_25887_length_3942_cov_3.582454, whole genome shotgun sequence.
ACCESSION   DAOQUC010000185 DAOQUC010000000
VERSION     DAOQUC010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3942)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3942)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3942
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25887_length_3942_cov_3.582454"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            142..1521
                     /locus_tag="PKH07_04480"
     CDS             142..1521
                     /locus_tag="PKH07_04480"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNT34235.1"
                     /translation="MKKLIVIAVVALIVAGFGWHYYSAKKKAPAEVERDSSAPEIVPA
                     EKGSIRMIVETTGRVVPSQEVEIKCKASGEIINLPLEVSDPVGKGDLIVQLDPQDEER
                     SYKRAEVSLKVSEARLNQARVDLDVSKRNLVTERTRAEAALQSSEAKAREVEAKLKRV
                     EQLVEKKMASAEELDVARTSFEQSQTEVKNAKARIEELATQTVSIKSKEQAVSIAEAQ
                     VESDKLSLSDAKKRLSDTTVLSPIDGVVAQKNVQVGTIIASGINNVGGGTTILTLVDL
                     SHLYVLASVDESDIGSIEPGQRARITVDAHPDAFFPGEVVRVATKGAVVSNIVTFEVR
                     IEVFGPRKALLKPEMTASVEITAVEKDDVISVPVAAVSRRRMEQFVTIQKEDGSTEER
                     IVETGVSDGDVMEIVSGLDEGEKVVVAKSSAFSPWQRDMDPARTARMRERMTMGMFGG
                     GGPGGRPSR"
     gene            1518..2183
                     /locus_tag="PKH07_04485"
     CDS             1518..2183
                     /locus_tag="PKH07_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008046882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNT34236.1"
                     /translation="MIIEMRDLRKTYQLGATTVCALDGVDIEVAEAEMVAITGPSGSG
                     KSTLMHIIGCLDRPDQGEYRLAGEDVSRLSKDRLAGIRNRRIGFVFQSFNLLPRLSAL
                     ENVEMPLLYAGRRDARARAGEALETVGLSDRMAHEPNQLSGGQRQRVAIARALVTDPA
                     ILLADEPTGNLDSNTGKEILAMFQQLNAQGRTIVVVTHDQNVARHCSRQIRLRDGKVV
                     PGD"
     gene            2187..3395
                     /locus_tag="PKH07_04490"
     CDS             2187..3395
                     /locus_tag="PKH07_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNT34237.1"
                     /translation="MLLWTILKVAIRSLLANKLRSFLAMLGIIIGVGAVISMLALGAG
                     AQKQVMDRVTAMGTNLLIIRPGSASRGGVRSGSRENLKIEDAEAILNEVENVVAVAPV
                     VMGRSQLKYLNKNSSTSITGSSSVYLEMRAFEVEKGRGFTSVEESRNARVAVLGATTV
                     ETLFGDQDPIGETIKVRGSNFRVIGVLKAKGDQGWFNPDDMVVMPYSTAMKQVVGQSY
                     LSEIDVKVSDTASLTTVQEKITELLRKRHRIRSEAEDDFNIRNQAEFVEMATDVTRTF
                     TILLGGIASISLIVGGIGIMNIMLVTVTERTREIGVRKAIGARDRDILRQFLLESILV
                     SALGGMLGVSAGIGTAELVSKFTQFGTVVELYSVILALAVSASVGVFFGYYPARRAAK
                     LDPIEALRYE"
     gene            3409..>3942
                     /locus_tag="PKH07_04495"
     CDS             3409..>3942
                     /locus_tag="PKH07_04495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34238.1"
                     /translation="MNKDCISTTNRKGLRALTNILVLLGLCIPCFADDVQQTGTTDFW
                     ASGTAEVSEASPEPKPGLQLTLSEAVLTALENNIAFQIERIRPEIRRTSEESELATFD
                     PSIAADLTHSRGWTRDDIENRAKDDKTRDHSTTAGVSVGQFFPTGTSVQLGLDQTFRS
                     SDASGSDSDRNTTNYDAS"
BASE COUNT          960 a          970 c         1170 g          842 t
ORIGIN      
        1 ctgtggctgc attgcgtagc gtgttgtgtc gtgaactggc tgcagtactg accagtcagt
       61 cgtctgtcat cacatacaac agaggttgtg agagccccac acgaatactc gggttacagc
      121 acttagtttg aggaatggaa catgaagaaa cttattgtca tcgcagtcgt cgcactcatc
      181 gtcgcaggtt tcggatggca ctactactcc gccaagaaga aagcacccgc ggaggtggaa
      241 agagactcgt ccgcaccaga gatagttccg gctgaaaaag gctcaattcg catgattgta
      301 gaaacaacag gacgcgttgt ccccagccag gaagtcgaga tcaagtgcaa agccagtgga
      361 gaaatcatca atttgcctct tgaggtcagc gaccctgtcg gtaagggaga cttgatcgtt
      421 cagttggatc ctcaggatga ggaacgatcg tacaagcgcg ccgaagtgag cctgaaagtc
      481 tccgaggctc gactgaatca ggcacgggtg gacttggatg tttccaagag aaatcttgtc
      541 acggagcgga ctcgtgccga ggcagcgctg cagtcttctg aagccaaggc tcgggaagtc
      601 gaggcaaaac tcaaacgggt ggaacaactt gttgagaaga aaatggcaag tgccgaggaa
      661 ctcgatgtcg ccaggaccag ttttgagcag tctcagaccg aggtaaagaa tgccaaggca
      721 cgcatcgaag agttggcaac gcagacggtc tcgatcaagt ccaaggaaca ggcggtttca
      781 attgccgaag cccaggttga atccgacaaa ctctccctgt ccgacgccaa gaaacgcctg
      841 agcgatacga ccgtgctctc ccccattgat ggggttgtcg cgcagaagaa tgttcaggtc
      901 ggcacgatca ttgcctcggg tatcaacaat gttggcggcg gaacaacgat tctgacgttg
      961 gtggatctct cacatctcta tgtgctcgcc tcagtagacg agagtgacat cggctcgatt
     1021 gaacctggac agagagcgag gatcacagtg gatgcacatc cggacgcttt cttccctgga
     1081 gaggttgttc gggtggcaac caaaggagcg gtggtctcca acattgtgac cttcgaagtc
     1141 cgaatcgaag tattcggacc taggaaggct ttgctcaagc cggagatgac ggcaagcgtt
     1201 gagatcacgg ctgtggaaaa ggacgacgtc atcagtgttc ctgtcgctgc ggtctcccga
     1261 cggcgcatgg agcagtttgt gacgatccag aaagaggatg gcagcaccga agagcgaatc
     1321 gtcgaaacgg gcgtcagtga tggagatgtt atggaaatcg tctccggact cgacgaagga
     1381 gagaaagtgg tggtggccaa gtccagtgca ttcagcccgt ggcagagaga catggatccc
     1441 gctcggacag cccgaatgcg ggaacgcatg acgatgggaa tgtttggagg cggggggcca
     1501 ggaggacggc catcacgatg attatcgaga tgcgtgacct tcgaaaaacc tatcagttgg
     1561 gcgcgacgac tgtctgcgcg cttgacggag tggatataga ggtagccgag gccgagatgg
     1621 tggcgattac cgggccctca ggcagtggca agtccacctt gatgcatatc atcggttgtc
     1681 ttgatcggcc tgaccagggc gagtatcgcc tcgcaggaga ggatgtctcg cgtctttcca
     1741 aagatcggct ggcaggtatt cgaaatcggc gcatcggctt cgtctttcag tcgttcaatc
     1801 tgctgccccg attgtcggcg ctggaaaatg tcgagatgcc cctgctttat gctgggcggc
     1861 gagatgctcg ggcacgagcg ggggaggcat tggagacagt cggtttgtcc gaccgtatgg
     1921 ctcatgagcc aaaccaactc tctggaggcc agcgtcagcg ggtggccatc gcaagagccc
     1981 tggtaacaga tcccgctatc ctcctggccg atgaaccaac aggcaatttg gacagcaata
     2041 ccgggaagga aatactggcg atgttccagc agttgaacgc acagggacgc accattgttg
     2101 tcgtcactca tgatcagaac gttgcacggc attgcagccg acagattcgc ctgcgggacg
     2161 gcaaggttgt gccgggagac tgaaccatgt tgttgtggac gatattaaag gtggcgattc
     2221 ggagtctgtt ggcgaacaaa ctccgttcgt ttctggcaat gctggggatc atcattggcg
     2281 ttggtgcggt gatctccatg ctcgcccttg gagccggtgc ccagaaacaa gtcatggatc
     2341 gagtaaccgc gatgggcacc aaccttctta ttatcagacc cggttccgct tcgagaggcg
     2401 gcgttcggtc gggatcccgg gagaatctga agatcgaaga tgccgaggcc atactgaacg
     2461 aggtcgaaaa tgtcgttgcg gtcgcgcctg tcgtcatggg acgctcacag ctgaagtacc
     2521 tcaacaagaa cagcagtacc agcatcacgg gttcttcaag cgtttatctg gaaatgcggg
     2581 cttttgaagt ggagaaagga cgtggattca cgtccgttga ggagagccgc aatgcacgtg
     2641 tcgctgttct tggagcaaca acggtagaga cgctctttgg agatcaagac cctattggtg
     2701 agaccatcaa ggtcagaggt tccaatttcc gcgtgatcgg tgttctgaag gccaaagggg
     2761 atcagggatg gttcaatccg gacgacatgg tggtcatgcc ctattcaaca gctatgaaac
     2821 aggttgttgg acagagttat ctctccgaga tagatgtaaa agtctccgat acggcaagcc
     2881 tgacgacggt acaggagaag atcaccgaac tgctccgaaa gagacaccga atccgctctg
     2941 aagcggagga tgatttcaac attcgaaatc aggcggagtt tgtcgagatg gcaaccgacg
     3001 tcactcgaac tttcaccatt ctcctgggcg ggatcgccag tatctccctg atagtgggcg
     3061 ggatcggaat catgaacatt atgctggtaa ccgtcacgga acgcacccga gagatcgggg
     3121 tgagaaaagc tatcggagca cgagaccgcg atattctgag acaattcctg ctggaatcca
     3181 tcctggtcag cgcgctcggg ggaatgctgg gagtttctgc cggtatcggc actgccgagc
     3241 tggtaagtaa attcacccag tttggtaccg tggtggagtt atacagcgtg atattggcac
     3301 tggcggtgtc tgcatctgtc ggtgttttct tcggatacta tcccgctcgg cgagcggcaa
     3361 aactggatcc catagaagct cttcgctatg aataggagat cgaatcagat gaacaaagac
     3421 tgcatatcca caaccaaccg gaaagggctg cgtgctctga cgaacattct tgtgcttttg
     3481 gggctgtgta tcccctgctt cgctgatgat gttcagcaaa ctggcaccac ggacttttgg
     3541 gcatcgggca ccgccgaggt ctcagaagcc tcaccagagc caaagcccgg gctgcaattg
     3601 accctttcag aagcagtact gaccgctttg gagaacaaca ttgcctttca gattgagcga
     3661 atcagacctg agatcaggag aacgtctgag gaatccgaac tggcaacttt cgatccgtca
     3721 atcgcggcag accttaccca ttcacgcggt tggacacggg acgatattga gaatcgtgcc
     3781 aaagatgaca agacccgtga tcattcgaca actgctggag tctcagttgg ccaattcttc
     3841 ccgacaggga cttcggttca actcggtttg gatcagacgt ttcgctcttc cgatgcctcg
     3901 ggcagtgaca gtgatcggaa cacgaccaat tacgatgctt ca
//
LOCUS       DAOQUC010000186         3941 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_25901_length_3941_cov_2.643592, whole genome shotgun sequence.
ACCESSION   DAOQUC010000186 DAOQUC010000000
VERSION     DAOQUC010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3941
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25901_length_3941_cov_2.643592"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            164..1048
                     /locus_tag="PKH07_04500"
     CDS             164..1048
                     /locus_tag="PKH07_04500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34239.1"
                     /translation="MRERIVKLGTIDLDLVETTPIVFKGKLYRFEYVRPGYSGNADGV
                     SFFRFVERDSGKASASFARDYHLGNVMVEGDTLYVTGTNKWDGDRVDLFVSDDMENWQ
                     SQVALELPGYGLFNTSLCRADEKYVLMFEVGRPPEVAGVRFTALFAVSDDLKKWNVLP
                     QECCYAKDRYTAPHCLRFLDGYFYNFYLEAVSGGYEQYVVRSRDLIQWESSPLNPVLR
                     ACDNDRIIANPSLTAEQKERIRTAVNINNSDIDFCEYNGTLVINYSWGNQHGVEHLAE
                     ARFDGTLKEFLTGWFPEV"
     gene            1081..1323
                     /locus_tag="PKH07_04505"
     CDS             1081..1323
                     /locus_tag="PKH07_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34240.1"
                     /translation="MDISTLRSFFMWCTVVNAGLLALSFLFIALAGEWIYRMHRRWFP
                     MSKENFSMAMYCFLALFKILILVFNLAPYIALLIMG"
     gene            1458..2831
                     /locus_tag="PKH07_04510"
     CDS             1458..2831
                     /locus_tag="PKH07_04510"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNT34241.1"
                     /translation="MVESRRRPSMLIVDDEPSVLELCKWVLKELDFEVCLAKSGKECL
                     VSASSVRPDFILLDVQLPDLDGLAVLEELVQKGLAHCPVVMMTGSPTVDMAVRAMKLG
                     AKDFLQKPFSNQKLLEIVREHMKTERIWSGDSRPHWAERIGLVGSSEKMRSVLKLVER
                     VAWNDVTIMLYGESGTGKELIARSIHSIGSRASEPFVPVDCSALATSIIESELFGHVK
                     GAYTGAHGNREGLLRVAGKGTVFLDEVGELPLDVQAKLLRVLQEKVVRPLGSEEFYFL
                     EARVIAASNRELQQAVREGKFREDLFYRLNVIPIHLPPLRERREDIPALADFFLKRYS
                     SRAYSAVRMSASAMQFLVQFDWPGNCRQLENAVQRALVLSDEDEIQISHLSQMNLTPN
                     ARQSVAGDVSSSLLESAERQTIVQVMNQTGWNKREAARLLGISKTTLYAKLAKFGIST
                     EGRQTLE"
     gene            3081..>3941
                     /locus_tag="PKH07_04515"
     CDS             3081..>3941
                     /locus_tag="PKH07_04515"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HNT34242.1"
                     /translation="MNAEERVEWIAEALLLVEPTDFQDLANVHTEFEKLIEWSKSVGR
                     TDVLTLSSRSLSLIERVILQETDDPVGALEQVGKNLSAIQNLVRDIGGDDLRTSSGQS
                     SDLSQNTTNQEDGESVSHPTGLPTHIDAQIFADFLARQGSVLERVEELVLVLEQGKGE
                     SVLAELRRIIHTLKGETKLLCLDEVQSLCHVTEDLLDSGCGSAAADRLLAVKDWLARA
                     FDAYSGKGALPESVEELLKELTDLVARNEAERTSDADVGKSSSDAMGLALPAPETEQK
                     EVVLEADPDLL"
BASE COUNT          900 a          888 c         1138 g         1015 t
ORIGIN      
        1 aagcagattc gagaggtcta tcagaagtcg catccgcggg cgggtcgaag atgacgaagg
       61 aacttgtccc aaggcagggc atttcaccat ttttcccaac ttcctgtgct ccggaacgga
      121 cagggtggta tcataatgca gattgtaatt gggaggttct attgtgagag agcggatagt
      181 aaaactgggt accattgact tggacttggt ggagaccact cctattgtgt tcaaaggaaa
      241 actctaccgt tttgagtatg tccgacctgg atattctggc aatgctgatg gggtttcgtt
      301 ctttcggttt gttgagcgtg attcagggaa agcctcggct tcctttgctc gggactatca
      361 tttggggaat gtgatggttg agggggatac tctctatgtt accgggacta acaaatggga
      421 cggagatcgg gttgatctct ttgtgtccga cgatatggag aattggcagt cacaggtggc
      481 acttgaactg cctggctatg gactcttcaa tacgtccctg tgtcgggcgg atgaaaagta
      541 tgtcctgatg tttgaagtgg gaaggccacc tgaggtagca ggtgttcgct tcacagctct
      601 ttttgccgtc tcagatgatt tgaagaaatg gaacgtgttg cctcaggaat gctgctatgc
      661 aaaagaccgt tacactgcac ctcactgttt gcgcttcctg gacggctatt tctacaattt
      721 ctacctggag gcggtcagcg gaggatatga gcagtatgtt gtgcgctctc gcgatctcat
      781 tcaatgggag tccagtccgc tgaatccagt tctgcgggct tgcgacaacg atcgaatcat
      841 tgccaacccc agtctgacgg ctgagcaaaa ggaacgcatc aggactgctg tgaacatcaa
      901 caactccgac atagacttct gcgaatacaa tgggactctc gttatcaact actcctgggg
      961 caatcagcac ggtgtggagc accttgctga agcacgcttt gacgggacac tcaaggagtt
     1021 tctgaccggc tggtttccgg aagtatgatc tgaaggcggc agagcctcag gaggaacatc
     1081 atggatatat caacccttcg aagtttcttt atgtggtgca ccgtggtgaa tgcagggctt
     1141 cttgcgctct cgtttctttt cattgctctt gctggggagt ggatctaccg gatgcaccgg
     1201 cgttggtttc ctatgtccaa ggagaacttc agtatggcga tgtactgctt tctagctctt
     1261 ttcaagatac tgatactggt gttcaacctt gctccctaca tcgctctgct gatcatggga
     1321 taggatccga gctgttgttc ggctcggact gggtctggaa agcagcgcag atgccaggct
     1381 gtattattct tcctgtccga tgtgcttcag cggcagaaag ccaaaacttg gcatgacgct
     1441 ctagaaagag gatatcagtg gtcgaatcgc gaagacgtcc cagtatgttg atcgttgatg
     1501 acgagccatc cgtcctggaa ctctgcaaat gggttcttaa ggaactcgac tttgaagtct
     1561 gtctcgccaa atctgggaaa gagtgtctgg tatctgcctc ttccgtcagg cctgacttca
     1621 ttttgctgga cgttcagctg ccggatcttg atggattggc tgttcttgaa gagcttgttc
     1681 agaaaggcct tgcacattgt ccagtcgtta tgatgacagg ctctccaacc gtggacatgg
     1741 cggttcgagc aatgaaactg ggtgcaaagg attttctgca gaaacctttc tcaaatcaga
     1801 aactcctgga aatcgtccgt gaacacatga agaccgaacg catctggtcg ggtgattcga
     1861 gacctcactg ggctgaacgg attggtttgg tcggaagttc cgaaaagatg agaagcgtgc
     1921 tgaaactagt cgagcgtgtc gcctggaatg atgtgacgat catgctttac ggggaaagtg
     1981 gaaccggcaa ggaactgatt gcccgttcga tacacagcat tggctcacgc gcttctgaac
     2041 ctttcgttcc tgtggactgt tcggcgttgg caaccagcat tatcgaaagc gagttgtttg
     2101 gtcacgtcaa gggagcctac accggggcac acggtaaccg tgaaggactg ctccgagtcg
     2161 cgggcaaggg taccgtcttt ctggatgaag tgggcgaact tccgctcgac gtgcaggcaa
     2221 aactgcttcg tgttcttcag gaaaaggtcg tcaggcctct cggctcagag gagttctatt
     2281 ttctggaggc ccgggttatc gcggcgtcca acagagaact ccagcaggcc gttcgtgaag
     2341 ggaagttccg tgaagatttg ttctatcgtt tgaacgtgat tccgattcat cttccgccac
     2401 tcagggagcg ccgcgaggat atccccgcct tggcagattt ctttctgaag agatattcat
     2461 ctcgggcgta ttctgctgtt cgaatgagtg cttcagcgat gcagtttttg gtgcagttcg
     2521 attggccggg caactgcagg cagttggaga atgctgtcca gcgtgcactt gtcttgtcgg
     2581 acgaagatga gattcagatc tcacatctgt ctcagatgaa tctcacgccg aacgcgagac
     2641 agagtgttgc cggggatgtt tcttcctcac ttcttgagag tgcggagcga cagaccatcg
     2701 tccaagtgat gaatcagact ggttggaaca agcgcgaggc cgcacgcctt ttgggtatca
     2761 gcaagacaac gctctacgca aagttggcca agttcgggat cagcacagaa ggccgtcaaa
     2821 cgttggagta gatccacgtc caatttcgct atgcggccag tttctggact agttggagag
     2881 aacagaacag aaactggtca cctcctatca tcccttcttg ttttcgttcg gtataacctc
     2941 ttcagtatca atgtgttgtg tgtttcacat tgagtgggca cagagattgc tcagcttgaa
     3001 agtagaatga gggatgacac cataggtcga acggatttca tacgcgcggt gttgtcaggc
     3061 agacgaaagg aacgatggag atgaatgctg aggagagagt tgagtggatt gccgaggcac
     3121 tgctcctggt tgaaccaacc gattttcagg acttggccaa cgtccatact gagtttgaga
     3181 agctcattga gtggtcgaag agcgttgggc gtactgatgt tctgactctt tcttcgagga
     3241 gtctctcttt gattgagaga gtcatcctgc aggagacaga tgatccggtc ggggcacttg
     3301 aacaagtcgg aaagaatctc tccgcgatcc agaatctggt gcgagatatt ggcggagatg
     3361 atttgcggac ttcttccggg caaagttcag atctgtccca gaacacaacc aaccaggagg
     3421 atggcgagag tgtttcccat ccaaccggcc ttcccacgca tatagatgca cagatatttg
     3481 ccgactttct tgcccgtcag gggagtgttc tggagcgtgt tgaggaattg gtgttggttc
     3541 tggagcaggg aaagggtgag agtgtcctgg cggaacttcg aaggatcatc cacacgctga
     3601 aaggagaaac taaacttctg tgcctggacg aagttcagtc gctgtgccat gtgactgagg
     3661 atctgctcga ctcgggctgc gggagtgctg ccgcagacag acttctggct gtgaaggatt
     3721 ggcttgctcg agcctttgat gcctattcgg ggaaaggagc gcttccggaa tcagtagagg
     3781 aactgctgaa ggagttgacc gatctggttg cgcgtaatga agctgaacga acgtctgatg
     3841 cagatgtggg taagagcagt tcagatgcga tgggtcttgc cttacccgct cctgagacgg
     3901 aacagaagga agtggtactt gaagcggatc cggatctgct g
//
LOCUS       DAOQUC010000187         3924 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_26038_length_3924_cov_2.921168, whole genome shotgun sequence.
ACCESSION   DAOQUC010000187 DAOQUC010000000
VERSION     DAOQUC010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3924)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3924)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3924
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26038_length_3924_cov_2.921168"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1409)
                     /gene="ilvB"
                     /locus_tag="PKH07_04520"
     CDS             complement(<1..1409)
                     /gene="ilvB"
                     /locus_tag="PKH07_04520"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HNT34243.1"
                     /translation="MIRRTGAQIIVELLERQGITQIAGIPGGANLPLYDALSQSTRIR
                     HILVRHEQSAGFMAQGMARVTGKPQVCFATSGPGATNLMTAIADAKLDSIPIIAITGQ
                     VPSALIGTDAFQEVDTYGMSLPITKHNFLVRSAEELLRIIPRAFRIAASGKPGPVLID
                     VSKDIQTQQLEFDAYPEPGRPDPSPVISEEQVRQAAAMINGASRPILCLGGGVIHAEA
                     GDLAQELIERSAIPTTMTFMGLGAIPSDHPLSLGMLGMHGARATNLALEECDLFIAIG
                     SRFDDRATGKVSEFCRNAQILHVDIDSGELDKIKRAHLGIEGDAKKFLETLLPWIDEN
                     PRHQWNATVDDLRRTFPSSFPDQDTPQSAYGLIRHVAQLVGDEAIVATDVGQHQMRTA
                     QAYPFQRPRQWLSSGGLGTMGFGLPTAIGAALAAPERQVVCFTGDGSLLMNIQELSTL
                     AELNANVKIILMNNRSLGLV"
     gene            2128..>3924
                     /locus_tag="PKH07_04525"
     CDS             2128..>3924
                     /locus_tag="PKH07_04525"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HNT34244.1"
                     /translation="MSHTLPYKNPSLPIEKRVEDLLARMTLEEKACQLNMLPNSWGDG
                     PSFMKDGTFHEPTAQANIGEHGIGSLHQREDKTSPRSLAEATNEVQRFLTTKTRLGIP
                     ALIICESLHGNAAPNCTIFPQALALASSWNAELVRQVGTAIAKECSSCGSTSGLSPVL
                     DVGRDPRWGRMEEGYGEDPHLVSRMGVAMVRGMQGDGDKVDKEHIIATPKHFAGHGSP
                     LGGRDSHQAGYGREFLREIALPGFEAAVREAKAGSIMAAYSDWCNVPCNASHELLTEI
                     LRDEWGFDGYVIEDMGAIGLLEKTHSVAKDYEDAVRLAVQAGVDQSFGGSVSEQVLNL
                     VRQGKLSESVVHEAAKRVIRAKFRLGLFENPYVAPDRAEAICGCQEHRDLARHAARES
                     IVLLKNENATLPLRKDVSAIAVIGPNAAKMETGDYSGWMTPLVSPLEGIRNAVSSNTT
                     VHYVEGCKVVGTTSADAEPVPTAYLTPKDTTRGLAPQAGASGITGEYFDNLDLRGEPK
                     LVRIDEMVSFTPGECRSPAADEGIPKDYSVRWSGTLTPEVSGRYRIGAAHSDGARLWI
                     NDKLLVDHWQDGAIATHTAEITFEANERYSIVL"
BASE COUNT          899 a         1092 c         1090 g          843 t
ORIGIN      
        1 acgagcccca gcgaacggtt gttcatcaga atgatcttca cgttggcgtt gagttctgcc
       61 agcgttgaca gctcctgaat gttcatcagg agacttccat ctcctgtgaa acagaccacc
      121 tgtcgttccg gcgctgccag tgcggcaccg atggccgttg gcagcccgaa tcccatcgtg
      181 ccaagaccgc cggacgacaa ccactgtcgt ggtcgttgaa atgggtatgc ctgtgccgtt
      241 cgcatctgat gctgacccac gtcggtcgcc acaatggctt cgtcgccaac gagctgtgcc
      301 acgtgccgaa tgagtccgta ggcggactga ggggtatcct gatccggaaa cgatgacggg
      361 aacgttcgcc gcagatcatc aaccgtggca ttccattggt ggcgcggatt ctcgtctatc
      421 cagggaagga gagtctccag gaacttcttt gcgtcacctt cgatgcccaa atgagcccgt
      481 ttgatcttgt ccaattctcc cgaatcaatg tccacatgaa ggatttgggc gttacgacag
      541 aactcggaga ccttacccgt ggcgcggtca tcgaaccgtg agccgatggc aatgaagaga
      601 tcgcactctt ccaaggcaag gtttgtcgca cgcgctccgt gcataccgag catccccagc
      661 gacagagggt gatcggacgg tatcgcaccc aatcccatga aggtcatcgt cgttggaatt
      721 gccgaccgtt cgatcaactc ctgcgccaaa tctcctgctt cggcatgaat aactccgcca
      781 ccgaggcaga ggattggtct tgaagcgccg ttgatcatcg ccgctgcctg acgcacttgt
      841 tcctcagaga tcaccggcga tggatcgggg cgtcccggct caggataggc gtcgaactcc
      901 aactgctggg tctggatatc tttggacacg tcaatcagca cgggaccggg cttgccggat
      961 gccgcaatgc gaaacgccct gggaatgatc ctcaagagct cttctgccga tctcaccagg
     1021 aagttgtgtt tggtaatcgg aagcgacatt ccgtatgtgt ccacttcttg aaaggcgtcg
     1081 gtgccaatca aggcgctggg aacctgaccg gtgatggcga tgatcgggat cgaatccaac
     1141 ttcgcgtcag cgatcgcagt catcagattc gtcgcgccgg gacctgatgt ggcaaaacac
     1201 acttgaggct ttccggtgac acgagccata ccctgcgcca tgaatccagc gctctgctca
     1261 tgtcgaacga ggatgtggcg aatccgagtg ctctgcgaca aggcatcgta caggggcagg
     1321 tttgccccac ccggtattcc tgcgatctgg gtgatgccct ggcgctccaa cagttccacg
     1381 atgatctgtg cgccggttct tcttatcatg aacttcgtct cctttggctc cccaggtgtc
     1441 gagcttggcg cgggtgggtt tcccaaaaaa caccaacccc cgcctggttc gcaccagcgg
     1501 gggctgtttt tttttcttct tttcagtcag tcccgctaag gcgcgacggc gtttgtcacg
     1561 cgtactacga cgagtacacg tacaacaacc ccaacgccct gcacctggca ggatgctgct
     1621 tgggaaaggg ttgtcaggcc atcacgcatc atcgaaacca acctcaaatc tcacaacgta
     1681 cagaaactct acttatccaa taaacgcaag tcaagcgttt tggtaactgg attgaactgg
     1741 ctcagttatg ctggtgatac agccaggata tgcttcggaa gcaaagcgga gtcttcggag
     1801 cgacgctacc gaagcccgct gtctggccgt tcccgcttcg gcagcgtcgc agagcctcta
     1861 ctaccgaagc atacctccac cccctccgta actgagccgg tacactggat tggcaacgca
     1921 cagtcacaat ctgttccttg gtgggaaagt gattgcatag aacaaactgc tccagatctg
     1981 cgccagcaac aagatgccca accctattgt tccaacctgt gcggcaatga ctctctctgc
     2041 ctgattgaca cttctccctc aagaaactac tatccgcaaa cgtctgaaaa cagacatcat
     2101 gttttatctg cgagaggaca acgcactatg tctcataccc ttccttacaa gaacccatct
     2161 cttcccatcg aaaagagagt ggaagactta cttgcgcgaa tgacactcga agaaaaagcc
     2221 tgccagctca acatgctccc caactcgtgg ggagacggtc cctcctttat gaaggatggt
     2281 acgtttcatg agcccaccgc acaggcaaac attggcgagc acgggatcgg ttctttgcat
     2341 caacgtgagg acaaaaccag cccgcgatca ttggcagagg caaccaatga agtacagcgc
     2401 ttccttacca ccaaaacacg cctgggtatt cccgctctga tcatctgcga gagccttcac
     2461 ggaaatgctg caccaaactg taccatcttt cctcaggctc ttgcactcgc aagctcctgg
     2521 aacgctgaac tggtacgcca agttggaacg gcaatcgcca aggaatgcag ttcgtgcgga
     2581 tcgacttcag ggctttcccc tgtgctcgat gttggcagag atcctcgctg ggggcgcatg
     2641 gaagaaggtt acggtgaaga cccacacttg gtctcaagga tgggtgtggc gatggtcaga
     2701 gggatgcagg gggatggcga taaggtggac aaggaacaca tcattgccac gccaaagcat
     2761 ttcgccgggc acggttcacc tctgggcggc cgtgacagcc atcaggcagg ctatggtcgt
     2821 gagtttctgc gggaaattgc gctccctgga ttcgaggcgg cggttcgcga ggcgaaggca
     2881 ggctcaatca tggcagcgta cagcgattgg tgtaatgttc cgtgcaatgc ctcgcacgag
     2941 ctcctgacgg agatacttcg tgatgaatgg ggatttgacg gctatgtcat cgaagacatg
     3001 ggagccatcg gtctccttga gaagacgcac tcggtcgcga aggactatga ggacgccgta
     3061 aggctggctg ttcaggccgg tgttgatcag agtttcggtg gctcagtctc tgagcaggta
     3121 ctgaatctgg tgagacaggg aaaactgtcc gaatcagttg tgcatgaagc cgccaagaga
     3181 gtgatcaggg caaagttccg tttgggactt ttcgagaatc cctacgtggc tcctgataga
     3241 gcagaggcaa tctgtggttg ccaggaacat cgcgatctcg cgcgtcatgc cgctcgggaa
     3301 agtattgtgc tgctgaagaa tgagaacgca acacttcctc tgcgcaagga cgtgagcgcc
     3361 atagccgtca tcggacccaa cgcagcaaag atggaaaccg gtgattacag cggatggatg
     3421 acacctctgg tttcgccgct tgaaggcatc cggaatgccg tgtcgtcgaa caccacagtc
     3481 cattacgtcg agggctgcaa agtggtcgga acaacgtccg cagatgccga gccggttccc
     3541 accgcttatc tgactcccaa agatacaacg agaggtcttg ctccacaggc aggcgcttca
     3601 ggaatcacgg gagagtattt cgacaatctg gacttgaggg gtgaaccgaa actggtgcgc
     3661 atagatgaga tggtcagttt cacgccggga gagtgccgat ccccagcggc cgacgaaggg
     3721 atccccaaag actattccgt acggtggagt ggcaccctga ctccagaagt gagtggacga
     3781 taccgaatcg gtgcggccca ctcggatgga gcacggcttt ggataaacga caagctgctt
     3841 gtcgatcatt ggcaagacgg agccattgcg acacacactg ccgagatcac ttttgaggcc
     3901 aatgaacggt attcgattgt gctg
//
LOCUS       DAOQUC010000188         3924 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_26039_length_3924_cov_2.700439, whole genome shotgun sequence.
ACCESSION   DAOQUC010000188 DAOQUC010000000
VERSION     DAOQUC010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3924)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3924)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3924
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26039_length_3924_cov_2.700439"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2952)
                     /locus_tag="PKH07_04530"
     CDS             complement(<1..2952)
                     /locus_tag="PKH07_04530"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HNT34245.1"
                     /translation="MKKKTVMVLGLVVCGLVLVAHSSSATPLAFHDAKGIYLSMRKEA
                     VSPETAIAALLEGPNEKEVALGLTTAIPKGTKLLSLDLLDDGALVNLSKEAAEGLNDS
                     KVEAVFAQFLWTLKAFELEGQVRLYIDGREPSSYLKPVAPALFKAPGDTEALATKSMG
                     IEAVGLSGKRITLSCGHGYVWTGTAWGTQRGITCGVEQEDFHNMRFGNYLITMLENDS
                     AYVQKTREHNFARGTSPHSNHDWWKESAYVWLYDQSYACNVYASYTGRCSDLVGASVN
                     RINDDIRARPLASNLDTRGNTDILVSIHTNALSGDCFGGSCPSGTDGFYIDDNGHETW
                     GVQSKDLAQKIQDDQIAAVQSAGFSYGHHGTTPALYGNFGEIRLPQRPAALIEIGFHD
                     SCDLDAPKINDPFFKACSAWGIYNGICKYFGNTPTYGMYASEYVSDTIPSTMVQGEQR
                     VVQVTFRNKSVWWDEAHAFRLGAVGDSDPFAATRHTISGTVENNGTYTFSFTMTAPSA
                     PSTYTTDWRMVRDGYAWFGATLSKQVVVSGTGNTATPTNTPTFTPTATPTSPATEIIV
                     DDGDGAYSQSGSWTYATGTAGTCYNQDYYWASTNVSAETAWAKWNPNLTGAAWYEIYV
                     MYRAGTNRANNAPYTVYHNGGSTVVNVNQTASGGTWVLLGTFAMDAGTSRYVKVSNGP
                     AEGSKVVIADAVRYVPVGNLDPTSTPTGQNTNTPTNTPTNTPTNMPTSTPTNTPTSGV
                     TPPADIIVDNSDGGFSVVSGSWSTGTSATDKYGADYRYATCDSTGDTVQWQANIPVTG
                     PYKVYCWYPQGSNRPNDAPYTVYYDGGNQTIDINQQYNGGQWNLIGIFNFTAGTYNVR
                     LTDIAQAGQVVMADAIKWAWHDTSPTATPTNTPVTTPEARGIWIDVYDVDAQSTLTTA
                     IDKAVQYNMNAVFPEIRFRADALYVPNRTDSTYPNPEPRSDQLSGAPPEFDPLQYAID
                     YGHSKGLEVHAW"
     gene            complement(3586..>3924)
                     /locus_tag="PKH07_04535"
     CDS             complement(3586..>3924)
                     /locus_tag="PKH07_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34246.1"
                     /translation="LSTPIAYIKSSESFFDPFSLFETMSPSDPRYQFHRYGYINMKFT
                     RQTNYPRVIDPSFETLIGKWRLDGYGPARVYGTGGWLYGPSYDPTNGTVSPGEIFRSQ
                     KYANHVQYGQ"
BASE COUNT          912 a         1054 c          982 g          976 t
ORIGIN      
        1 ccaggcgtga acttcgagtc cctttgagtg tccgtagtca atggcatact ggagcgggtc
       61 aaactcagga ggagctccgc tcagctgatc ggaacgcggc tcaggattcg gataggtcga
      121 gtccgtcctg ttcggaacgt acaacgcatc cgccctgaat cgaatctctg ggaacaccgc
      181 attcatattg tactgaaccg ccttgtcaat tgccgtcgtc aacgttgact gtgcatccac
      241 atcgtacacg tcaatccaga tgccacgggc ttccggagtc gtcacaggag tatttgttgg
      301 cgtcgccgtc ggagacgtgt cgtgccatgc ccatttgatc gcgtctgcca tgacgacctg
      361 gcccgcctga gcaatgtctg taagcctgac gttgtaggtg cccgccgtga agttgaagat
      421 gccgatcaga ttccactgac cgccattgta ctgctggttg atgtcaatgg tctgattccc
      481 accatcgtaa tacactgtgt aaggagcatc attcggacgg ttgctgccct gtggatacca
      541 gcagtagact ttgtatggac ccgtcaccgg aatgttagcc tgccactgaa ctgtatcacc
      601 ggtagagtcg catgtggcgt agcggtaatc cgcaccatac ttgtcagttg cggatgtacc
      661 tgtagaccaa ctgccactga cgacggagaa tccaccgtca ctgttgtcta cgatgatgtc
      721 tgctggtggc gtcacacctg aagtgggcgt attggtcgga gtgctggtcg gcatgttcgt
      781 cggagtgttg gttggtgtgt tggtcggcgt attggtattc tgccccgttg gtgtgctggt
      841 cgggtcaagg tttccgacgg gcacatatcg aaccgcatcg gcaataacaa ccttggaacc
      901 ttcagcagga ccattgctca ctttgacata tcgggatgtt cccgcatcca tggcaaatgt
      961 gcccaacaga acccaggttc caccactggc agtctggttg acgttgacaa ccgtgcttcc
     1021 accattgtgg tagacagtgt aaggcgcatt gttggctctg ttagttcccg ctcggtacat
     1081 gacgtaaatc tcgtaccaag ctgcaccggt gaggttggga ttccacttcg cccaagccgt
     1141 ctcagctgag acgttagtgc tcgcccagta ataatcctga ttgtagcacg taccggcagt
     1201 ccccgttgca taggtccaag aaccggactg gctgtaagcg ccgtctccat catcaacaat
     1261 gatctccgta gcaggactgg tcggagttgc tgttggcgtg aaggtaggag tgttcgtcgg
     1321 tgtcgccgta ttgccggtgc cactgactac gacctgctta ctcagagtgg ctccaaacca
     1381 tgcatatcca tcacgcacca ttctccagtc cgttgtgtag gtcgaaggag cggatggagc
     1441 cgtcatggtg aaactaaacg tatatgttcc gttgttctca accgttcccg aaatcgtgtg
     1501 tcgggttgca gcaaagggat cagaatcacc gacggcacct agtctgaatg catgagcctc
     1561 atcccaccac acagacttgt ttcggaacgt cacttggacg actcgctgtt cgccttgaac
     1621 cattgtgctg ggaatcgtgt cagagacgta ctcggaagcg tacataccgt aagtgggagt
     1681 attgccgaag tatttgcaga ttccgttgta gattccccat gcactacaag ctttgaaaaa
     1741 cgggtcgtta atctttggag cgtcgagatc acaactgtca tggaagccaa tctcaatcag
     1801 agctgcgggg cgctgtggca gtcggatttc accgaagttt ccataaaggg caggtgtggt
     1861 tccatgatga ccatagctga atcctgcgga ttgcactgca gcaatctgat catcctggat
     1921 cttctgagca agatccttgc tctgaacgcc ccaagtctca tgtccattgt catcaatgta
     1981 aaagccatct gttcccgacg ggcaactgcc accgaaacag tcacctgaaa gagcatttgt
     2041 gtgtatggac acaaggatat ccgtgttccc tctcgtgtcc aggttcgaag ccagaggacg
     2101 cgcacggatg tcatcgttga ttcggttgac actcgcaccc accaggtcag agcaccgtcc
     2161 cgtgtaactg gcatagacat tgcaggcgta gctctggtca tagagccaga cgtatgcgga
     2221 ttccttccac cagtcgtggt tgctgtgggg ggacgtacca cgagcaaagt tgtgctcacg
     2281 ggtcttctga acgtaggcgc tgtcattctc cagcatcgta atcaggtagt ttccaaaacg
     2341 catgttgtgg aaatcttcct gctcgacacc gcaggtaatg cctcgctgcg ttccccaggc
     2401 ggtacctgtc cagacgtagc cgtgtccgca tgacagggtg atgcgcttgc cactcaaacc
     2461 aactgcctca attcccattg attttgtggc gagtgcctca gtatcccccg gtgccttaaa
     2521 aagtgcgggg gcaacaggct tcagataact tgaaggctct cttccgtcta tatagagacg
     2581 cacctgcccc tcaagctcga acgcctttag tgtccacaag aactgcgcga agactgcctc
     2641 aaccttgctg tcgttaagac cctccgctgc ttccttggac aagttgacca gagcaccgtc
     2701 atcgaggaga tccagactca acaacttggt gcccttgggg atggctgtcg tcagcccaag
     2761 cgccacttcc ttctcgttcg gcccttccaa caatgcggct attgccgtct ccggagagac
     2821 tgcttctttg cgcatcgaga ggtagattcc tttggcatca tgaaatgcca gtggagttgc
     2881 cgaagacgaa tgggccacaa ggactagacc gcagacaacc agtcccagga ccatcactgt
     2941 ctttttcttc atgattacat tcctttcatc accaaaacaa aaccatcaga acaacacgaa
     3001 tcattgccca gcccaacttt tctttcctcg atgccctcct ttctttcaca ttgtatccat
     3061 tggaaatgat aaattcgtac caactttggt acaaattcag tcaaaagaat tgaaagagac
     3121 attgtgccca ttttcatcat ctctatggaa cttgtcgtcc caagcgtccg gaaaaccgtc
     3181 ggttctggag tgcatgatac ggatactttt ctcttgggag acgatcgcca tcaggctgca
     3241 aggacaggga acttatgcgt ttaagatgca cgttggatga acgctccgtc atgcgccttg
     3301 tgagatcgaa aacgtctgcg cactgcataa catcccatcc cctcatcaga accgtttttc
     3361 tccgaagcct gagaaaccct tgactcgaca tactcctctt aacagcgcat cacaatccaa
     3421 accaccacaa cacataaatc aactcattgc tgatagtgta ccgccagaaa ctggcggttg
     3481 tcaaatcttt tttgaaagac agaccaataa caggacaatg cagataacat tcataaaatg
     3541 aagttgttat gaatgagaaa gccctctatc cagaacactc tttcactact ggccatactg
     3601 gacgtgattc gcgtatttct ggcttcggaa tatctcccca gggctgactg tgccatttgt
     3661 agggtcatag gaaggaccgt acagccaacc gccagtccca tagactctcg caggcccata
     3721 tccatcaagt ctccatttgc cgatgagagt ctcaaaactt gggtcaatga cccgaggata
     3781 gttcgtctgc cttgtgaact tcatgttgat gtacccgtat cggtggaact ggtatctggg
     3841 atcgctcgga ctcatggtct caaagaggga gaatggatcg aagaacgatt ccgaactctt
     3901 gatataggcg atgggagtgc tcaa
//
LOCUS       DAOQUC010000189         3907 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_26189_length_3907_cov_2.348910, whole genome shotgun sequence.
ACCESSION   DAOQUC010000189 DAOQUC010000000
VERSION     DAOQUC010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3907
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26189_length_3907_cov_2.348910"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(88..489)
                     /locus_tag="PKH07_04540"
     CDS             complement(88..489)
                     /locus_tag="PKH07_04540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34247.1"
                     /translation="MNRLTLRGAGFVIAAVVCCSSSWSTILDTFDAIGGWKDPNYSGS
                     TSADPACTFTIVSSPRREGTGAGDLYYVWGSGSFIREVNSTQPVIPADCVLSVWIYGD
                     SSGNEVRLCVRDQEGELFTNDYTTIDFTGWH"
     gene            complement(645..>3907)
                     /locus_tag="PKH07_04545"
     CDS             complement(645..>3907)
                     /locus_tag="PKH07_04545"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HNT34248.1"
                     /translation="HYKIVHAGGETHYYLDQRMRRFTWIPIGQYYFESGTGGKVVLYN
                     DSTSTSHYISADAVRFGGGLGVVSRGNSGVNGSPRTDECSRYNIQFSGCPNYDGSGND
                     ESDTWTYSPRFGWWLRDAAQAYGAPSQNSVFLSSHTNAGGGSGMGTYVYDGYQNTWHD
                     RLRNYIHNEVRDDIRGAWNPSFIDRGKHYGNYGENNPNNVGNTMPIVLGEWLFHDSAV
                     DMAQYHDPKFRNVLARAIYQGIVKFWAAENGSPSTLLPESPTNFRCEVQSTTSVKLTW
                     AAPAADAQGGNAATGYKYYRSTHGRGFPAGTAQTGTSVTITGLTPGTTYYFKITATNA
                     GGESFPTEVLAAKIPAAAGTPKLLIVNGFDKLDKSTRVRISYSGDTLYRQNFMKMNTH
                     DYIVEHAKAIDAYGTALAFDSCEHEIIDAGTINMVNYAGVIWIGGLQAEAWTDDPTVD
                     TSLNGSIQSRLQTYLNAGGKLFISSAELAWDLDRSGTTTFVDNYLKANYSADSAGTYT
                     AVAKAGSIFDGLGSMTFDNGTGNTYNVVWADVINPVGGSSVAMEYGSAGSGSNAAIQY
                     SGTYKLVYMGFPFETLTSVTKRNDVMTRVLNFLSVAAATPTNTPTRTPTSTPTRTPTN
                     TPTNTPTNTPTATNTPTNTPTNTPTKTPTNTFTNTPTNTPTNTPTNTPTATNTPTNTP
                     TNTPTATNTPTNTPTNTPTATNTPTNTPTNTPTATNTPTNTPTNTPTRTPTNTFTNTP
                     THTPTNTPANRVLLDSFDSIGSWKDPNYSGSTNADPACTFTIVSSPKREGTGAGDLYY
                     VWGTGTFIREVNNVLVIPARSVLNVWIYGDNSGHEVRLCVRDAESELFVNAYTVINFS
                     GWYELRWDLDNDPFYWYANAGDRLLTGDLKLDSIQIHKVTSQETGHLYFDEATYDEFI
                     PTPTPTNTATNTPTRTPTNTPTNTPTVTPTATPTRTERIIDDGDSGYTKSGTWTYVSS
                     GGAEPLNGDYNWTNAKTTPDAWSRWTPDMSGPGNYLVYVRYRQGTNRVTNAPYTVYYN
                     GGSQTVTVNQQISDGTWVLLGTFNFLQGTSGYVQLDNGPTSGGTVVIADAVKFTPQ"
BASE COUNT          810 a          972 c         1121 g         1004 t
ORIGIN      
        1 gctatcccac ttgggatcgc cggtgatgag cctgtcaccc gaattggcgt accaggaaaa
       61 cgcatcgtag ggcagttgcc atctcagtta atgccatccg gtgaagtcaa tcgtggtgta
      121 gtcattggtg aacagttccc cttcttggtc acggacacag agtcgaactt cgttgcctga
      181 actgtctcca tatatccaga cagagagaac acagtccgca ggaatgaccg gctgagtgct
      241 gttgacctcg cggatgaagc tgcccgagcc ccagacatag tacagatcac ccgcgcccgt
      301 gccttcccgt ctcggggaag acacgatggt gaaggtgcaa gccgggtcag cactggttga
      361 gccagagtag tttgggtctt tccagccgcc gatcgcgtcg aaagtgtcca atatggttga
      421 ccatgacgaa ctgcagcaga caaccgccgc gattacaaac cctgcgccgc gcagtgtcaa
      481 acggttcatc agttcaactc cttttgaaag agaggatcac ggtgcaatcc ttgccgggct
      541 caagcactgg cagatggtct ctctgtcgaa cgcatttggt caagaacggg gaaggaagcc
      601 caggaaccat ctgctacctt ccccgtcgaa tgataagatc tcccctattg gggtgtgaac
      661 ttcacggcat cagcaataac aacggtgcct cctgacgtgg ggccgttgtc cagttgcacg
      721 tagccactgg tgccttgcag gaagttgaat gttcccaaga gtacccatgt gccatcgctt
      781 atctgctggt tcacagtgac cgtctgtgag ccaccgttgt aatagacggt gtaaggggcg
      841 ttggtaacac ggttggttcc ctgtctgtag cgaacataga ccaggtagtt gccaggaccg
      901 ctcatatcgg gtgtccatcg tgaccaagcg tcaggtgtgg tcttggcgtt tgtccaattg
      961 tagtcaccat tcaacggttc ggcaccgcca ctggacacat aggtccacgt tccactcttg
     1021 gtatagcccg aatcgccgtc gtcaatgatc cgttctgttc gagtgggcgt cgccgtaggg
     1081 gtgaccgtag gcgtattggt cggcgtgttt gttggtgttc gcgtgggcgt gttggttgct
     1141 gtattggtcg gtgttggcgt tgggataaac tcgtcgtaag tcgcctcgtc aaagtacaga
     1201 tgaccggtct cctgagaagt gaccttatga atctggatgc tgtccaactt cagatcgcca
     1261 gtgaggagcc ggtcgcccgc gttggcgtac cagtagaagg gatcgttgtc caaatcccac
     1321 cgcaactcgt accaaccgct gaagttgatg acggtgtagg cattgacgaa gagttcactc
     1381 tcagcgtcac gaacacagag cctgacttca tgtcccgagt tgtcgccgta tatccagaca
     1441 ttcagtacgg aacgtgccgg aatgacgagc acgttgttca cctcacggat aaaggtaccc
     1501 gttccccaaa catagtacag atcgcccgca ccggtaccct cgcgttttgg ggatgacaca
     1561 atggtaaagg tgcatgcggg atcagcattg gtggagccag agtagtttgg atctttccaa
     1621 ctgccgatgc tgtcgaacga atccaacaga actctgttgg cgggtgtatt ggtgggagtg
     1681 tgtgtcgggg tgttggtaaa cgtatttgtt ggagttctgg tcggtgtgtt cgtcggcgta
     1741 tttgttggcg tattggttgc cgttggagtg ttggtcggtg tgttcgttgg agtgttggtc
     1801 gccgtcgggg tattcgtcgg tgtgtttgtt ggagtgttgg tcgccgtcgg ggtattcgtc
     1861 ggtgtgttcg ttggggtgtt ggtcgccgtg ggagtgttcg ttggggtatt cgtgggagta
     1921 ttggttggtg tgttggtgaa cgtatttgta ggagttttgg tcggcgtgtt tgtgggagtg
     1981 tttgtcggtg tgttggtcgc cgttggagtg ttcgtcggtg tattcgtggg agtattggtt
     2041 ggcgttctgg tcggagtgct tgtgggtgtc cttgtcggcg tattggtcgg ggtcgctgca
     2101 gcaacgctca agaagttcag aacgcgcgtc atcacgtcgt ttcgtttagt gacactggtc
     2161 agagtctcga atggaaaccc catatagaca agtttgtagg ttccgctgta ctgaattgcc
     2221 gcattgctgc cggagccagc gctaccgtac tccatcgcca ccgaagatcc gccgactggg
     2281 ttgatgacat ctgcccagac gacgttgtaa gtgttccctg tgccgttgtc gaaggtcata
     2341 ctgccgagac catcaaagat cgatccggcc ttggcaacgg cagtgtatgt tccagcgctg
     2401 tctgcggagt agtttgcctt gaggtagttg tccacaaagg ttgtggttcc actcctgtcc
     2461 aagtcccaag ccaactcggc actggagatg aacaatttgc cgcctgcatt gagatacgtc
     2521 tgaagtcttg actggatgct gccgttgagc gaagtatcca ccgtgggatc gtctgtccac
     2581 gcctctgctt gcagaccacc aatccagatg actccagcgt aattgaccat gttgattgtc
     2641 ccagcatcaa tgatctcgtg ttcgcatgaa tcgaatgcaa gtgctgtacc gtaggcatcg
     2701 atggctttgg cgtgctcaac gatgtagtcg tgcgtgttca tcttcatgaa attctgtcgg
     2761 tacaatgtat cgccactgta agagatccga acacgtgtgg acttgtcgag tttatcgaat
     2821 ccgttaacga tcaacaactt tggagtgccc gctgcagcag gaatcttggc agcaaggact
     2881 tcggttggga aactctcacc acccgcgttg gtggcggtaa tcttgaaata atatgttgtt
     2941 ccaggagtta agccggtaat tgtaacactg gttcctgtct gagctgtccc tgcgggaaac
     3001 ccacgtccat gggtgcttcg atagtacttg tacccggtcg cggcgtttcc accctgagcg
     3061 tcagccgcag gagcagccca cgtcaacttg acacttgtcg tgctctgaac ctcgcaacga
     3121 aagttcgttg gggattcggg taacagcgta ctcggagaac cgttctcggc tgcccagaac
     3181 ttcacaattc cctgatagat cgctcgggca aggacattgc gaaatttcgg atcatgatat
     3241 tgagccatat ccaccgcact gtcatggaac aaccattcgc ccaacacaat cggcatggtg
     3301 ttaccaacgt tattgggatt gttctctcca tagttcccat agtgcttgcc acggtctatg
     3361 aagctcggat tccaggcacc tcgaatatca tcacgcactt cgttgtgaat gtagttgcgc
     3421 agccgatcgt gccaagtgtt ctgatatcca tcatagacgt aagtgcccat cccgctaccg
     3481 cctccggcgt tggtatgaga actgagaaag actgaattct gcgacggtgc accgtaggcc
     3541 tgcgcggcat ctcgcaacca ccaaccaaat cgggggctat aggtccatgt atcagactca
     3601 tcgttgccac tgccatcgta gttgggacat ccagaaaact ggatgttgta tcgggagcat
     3661 tcatcggttc ggggacttcc attcactccg gaattgcctc gggagacaac tccgagaccc
     3721 ccgccaaacc gaacggcgtc tgctgagatg tagtgagaag tcgaggtgga atcattgtac
     3781 aaaactactt tgccaccagt accagattca aagtagtact gcccaatcgg aatccaagtg
     3841 aagcgtctca tgcgttggtc aagatagtag tgtgtctctc cccccgcatg gacgatcttg
     3901 tagtgtg
//
LOCUS       DAOQUC010000190         3906 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_26198_length_3906_cov_3.120748, whole genome shotgun sequence.
ACCESSION   DAOQUC010000190 DAOQUC010000000
VERSION     DAOQUC010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3906)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3906)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3906
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26198_length_3906_cov_3.120748"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..829)
                     /locus_tag="PKH07_04550"
     CDS             complement(<1..829)
                     /locus_tag="PKH07_04550"
                     /inference="COORDINATES: protein motif:HMM:NF019346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNT34249.1"
                     /translation="MNLVPEKAEQFRRDFEALETEIGKVIVGQSVLIRHVLVALIARG
                     HVLIEGLPGLGKTLLIRTLGRILDLNFSRIQFTPDLMPSDILGTEVLDAVNPKGRRFE
                     PGPLFANLVLADEINRATPKTQSALLEAMQEGNVTFLGQCYTLSQPFLVMATQNPIDL
                     EGTYPLPEAQRDRFFCQLNVPFPDSAELIAIADRTTGVQETDVRTVLDGHRILEMASF
                     VREVPAAEEIKRFAAALVLATQPGSSTAHPAAQRLIRFGASPRGMQALLMAGKVLALL
                     "
     gene            complement(826..1947)
                     /locus_tag="PKH07_04555"
     CDS             complement(826..1947)
                     /locus_tag="PKH07_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34250.1"
                     /translation="MTERDRVRAVVHSCLARWRWASFINALAQAVIALLVAAFVLTGV
                     SKLIFVPRAVWMVAWFGLGVVLLARVACLWHPPRMQRALRVIDERLGLKDALVTALEL
                     LSSKDSSWHECIFSDVLNRFASVDVRKACAMMPSRRVAALAALSVVCLGVIHLVPSRN
                     VLSVQLLDHIQQLSEDSKLLAESLMRSKPGPRARSIAFRALELLERIQEFPSSVAEEE
                     VSQLIAELQSYPPMHAKSSAGDTRSGSGTGSGSGTAKGSGISSQNLAQFQKALADFRS
                     SIQREQQPSLQEPTIVVNPPDIAEASPRESQVGATTSNPLLERSRKSILDKLVQRPSV
                     DEIPVQPYEIQEEPIPWNRIPLWQQPIVRRYFSRDKDSP"
     gene            complement(1944..2642)
                     /locus_tag="PKH07_04560"
     CDS             complement(1944..2642)
                     /locus_tag="PKH07_04560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34251.1"
                     /translation="MHEAEMEKSLNPLSECHPHRLGAFSLAMLVHVTLMGLTLWIQLY
                     DSSFSMAALTAGDLGRRLWQTLGAIEGGLLLAVIPFLCVEAVFAEEIDGKSRRAGSGR
                     WCVLSLSATIPFYLYAETRLGAVGMSVLFLIKIHWLLWALVVALCCTIVARLSADADQ
                     AFLRCYELTVVLLVCLFLLSSRLGFLWPWGAHGRYFQQYRSPSWLMTVAGLAVAAVLA
                     TVARVIRDKRRCLP"
     gene            2834..>3906
                     /locus_tag="PKH07_04565"
     CDS             2834..>3906
                     /locus_tag="PKH07_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010989505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HNT34252.1"
                     /translation="MNPSEILETIRMIREEHLDVRTVTLGISLRDCSHPDASAAQEKI
                     LQKITHVARDLVRVCEDIAVEYGIPIINKRIAVTPISLVAQTNEQSDFVEFARTLDRA
                     ARTVGVNFLGGYSALVHKGYAKGEKEFLDSIPKAMAETERVCASVNVATTRSGINMDA
                     VRQMGEVIKATAQLTKDRNCIGCARLVVFANAVEDNPFMAGAFHGIGEPECAINVGVS
                     GPGVIRAALAKTRGEPFDVVAETIKRTAFKITRMGQLVAQEASARLNVPFGIVDLSLA
                     PTPAVGDSVARILEEMGLEACGVPGTTAALALLTDAVKKGGTMASSHVGGLSGAFIPV
                     SEDEGMIESVRKGILSLEKLEAM"
BASE COUNT          958 a         1075 c         1075 g          798 t
ORIGIN      
        1 ccagaagcgc aaggactttg cccgccatga ggagtgcctg cattccgcgc gggctggctc
       61 caaacctgat tagccgctgt gcggcgggat gggcggttga agaaccgggt tgcgtggcaa
      121 gaaccagcgc ggcggcaaac cgtttgattt cctctgcggc ggggacttcc cgcacgaagc
      181 tcgccatctc cagaattcgg tgcccatcaa gtactgttcg aacgtcggtt tcctgaacgc
      241 ccgtggttcg gtcggctatc gcaataagtt ccgcactgtc gggaaaaggc acattcagtt
      301 ggcagaagaa gcggtcgcgt tgcgcttccg gcagtggata cgttccttcc aggtcaatgg
      361 gattctgggt cgccatgacg agaaacggct gcgacaacgt atagcattgg ccgagaaatg
      421 tgacgtttcc ttcctgcatt gcttccagca gtgcagactg cgttttcgga gtcgctctgt
      481 tgatctcatc ggcaagcaca agatttgcaa agagagggcc cggctcaaat cggcgtcctt
      541 tgggattgac tgcatcaagc acttcagtac ccaagatatc cgatggcatc aaatccgggg
      601 tgaattggat tcgggaaaaa ttcagatcca gaatcctgcc aagtgttctg atgagaagtg
      661 ttttccccaa gccgggtagt ccctctatca gaacgtgacc tcgggcaatc aaggcgacga
      721 gaacgtgtcg aatcaggacg ctctgtccta cgatcacctt gccgatctcg gtttcaagcg
      781 cctcaaaatc gcgtcgaaat tgctctgcct tctcaggtac taaattcatg ggctgtcctt
      841 gtctctcgag aagtatctcc gcacaatcgg ctgttgccaa agcggaatcc gattccacgg
      901 aatgggttct tcctggattt catagggctg tacgggaatt tcgtcaactg aaggtctctg
      961 aaccaacttg tccagtatgg actttctcga acgttcgagc agcggattcg aggtcgtcgc
     1021 tccaacctga ctctctcttg gactcgcctc tgcgatgtca ggcgggttga caacaatggt
     1081 cggctcctgc aaagagggtt gttgctcccg ctggatactt gaacgaaagt cggccaatgc
     1141 cttttgaaac tgagccagat tttgagacga gatgcccgat ccttttgcgg ttccagatcc
     1201 tgaacctgtt cctgatcctg agcgcgtgtc ccctgctgag ctctttgcgt gcattggggg
     1261 atacgactgc agttccgcta tcaactgcga gacttcctct tcggcgacgc tcgatggaaa
     1321 ctcttggatt cgctccagga gttccagtgc ccgaaacgca atggagcggg cacgaggacc
     1381 aggcttggag cgcatgagag attcggccag aagttttgaa tcctcactca gttgctgaat
     1441 atgatcgagc agttgtactg acaatacatt cctggacggc acaaggtgaa tgactccaag
     1501 acaaaccaca ctcaacgcgg ccaacgcagc aactcttcgt gaaggcatca tggcacaagc
     1561 cttccgaaca tccacagatg caaatcggtt cagcacgtca gaaaagatac attcatgcca
     1621 ggacgagtcc ttactcgaca ggagttccaa ggccgtcacc agtgcatctt tgagaccaag
     1681 gcgttcatcg atcactcgga gtgcacgttg catccttggt ggatgccata ggcaggccac
     1741 tcgagccaag agcacgacac cgaggccaaa ccatgccacc atccaaactg cccgcggaac
     1801 aaagataagt ttcgaaacac cggtcaacac aaatgccgca acaagcaagg caatgacagc
     1861 ctgtgccaga gcgttgatga acgacgccca tcgccatctg gccagacaag agtgaacgac
     1921 cgctctaacc cgatctcttt cagtcatggc agacaccgcc gcttgtctcg aatgactcga
     1981 gcaactgtgg caagcactgc agcaacagcc agacctgcaa ccgtcatcaa ccaggacgga
     2041 gatctgtatt gctggaaata tcgtccatgc gcaccccaag gccagagaaa ccccagccgg
     2101 gaactgagaa ggaacagaca taccaggagc accacggtca actcgtagca acgaagaaag
     2161 gcctgatccg cgtcggctga caatctggcg acgattgtgc agcaaagcgc caccacgagt
     2221 gcccacagaa gccaatgaat cttgatcaga aagagcacag acatcccaac tgcacccagg
     2281 cgcgtctcag catagagata gaaaggaatt gtcgcgctca gtgacagaac acaccatctc
     2341 ccggagcccg cgcgccgaga cttcccatca atctcctcag caaagacggc ctcgacacag
     2401 agaaaaggaa tgacggcaag aagcagtccg ccttcgatgg ctccaagggt ctgccaaagt
     2461 cgtcgtccca gatcaccggc agtcagtgca gccatcgaga aggacgagtc gtagagttga
     2521 atccagagag tcaaccccat cagcgtcaca tgcaccagca tcgcgaggga gaaggctcca
     2581 aggcgatgag gatgacactc agacaggggg ttcagactct tctccatctc tgcctcgtgc
     2641 atcgtgaatg tccttccata accgtctgca tagtataata cgccagaaac ggatcaggtc
     2701 gtgttatttt cagaccccga gcacaaacat tgcgtccgtc tgcactgccg tatatcatcc
     2761 acgcatgaaa gtggaacggg aagcgcccgt gacgaacgat ctacctatgg gcgcatgagg
     2821 agcaaagagc gtgataaatc catccgaaat ccttgagaca atacgcatga tccgcgaaga
     2881 gcatctcgac gtccggacgg tcactctggg catttcgctg agagactgct cacatcccga
     2941 tgcttccgcc gctcaggaga aaatcctcca gaagatcact catgtggcga gagatctcgt
     3001 gcgcgtatgc gaagatatcg ccgtcgagta cggaatcccg atcatcaaca agagaattgc
     3061 cgtcacaccc atctcccttg tcgctcagac gaacgagcag tctgactttg tggagttcgc
     3121 gcgaacactc gatagggcgg ctcgaacggt tggcgtgaat tttctcggcg ggtactcggc
     3181 gctggtacac aaaggctacg ccaaaggaga gaaggagttt ctcgattcaa ttcccaaggc
     3241 aatggcagaa accgaacgcg tctgcgcctc ggtcaacgtg gcaaccaccc gttccggaat
     3301 caacatggac gccgtgcgtc agatgggaga ggtgatcaag gcaacggcgc agctgacaaa
     3361 ggatcggaac tgcatcggtt gtgctcgtct ggttgttttt gccaacgcgg ttgaggacaa
     3421 tccgttcatg gcaggagcat ttcacggcat tggagagccg gagtgcgcca tcaacgttgg
     3481 cgtgagtgga ccgggagtca tacgagcagc acttgccaaa actcgtggtg aaccattcga
     3541 tgtggtggcg gagaccatca agcgaaccgc cttcaagatc acccgaatgg ggcagctggt
     3601 cgcacaggaa gcgtcggcgc ggttgaatgt tccgtttggc attgtggact tgtcgctcgc
     3661 ccccactccg gcggtgggtg atagcgtagc gcgcattctt gaagagatgg gcttggaagc
     3721 gtgcggtgtt ccgggcacaa ccgcggcact ggctctcttg accgatgcgg taaagaaggg
     3781 tggcaccatg gcgtcatcac acgttggcgg cttgagcggt gcctttattc cggtcagcga
     3841 agatgaaggc atgattgaat cggttcgcaa gggcatcttg agtctggaga aactcgaagc
     3901 gatgag
//
LOCUS       DAOQUC010000191         3905 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_26208_length_3905_cov_3.860000, whole genome shotgun sequence.
ACCESSION   DAOQUC010000191 DAOQUC010000000
VERSION     DAOQUC010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3905
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26208_length_3905_cov_3.860000"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2062
                     /locus_tag="PKH07_04570"
     CDS             <1..2062
                     /locus_tag="PKH07_04570"
                     /inference="COORDINATES: protein motif:HMM:NF019552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HNT34253.1"
                     /translation="PVVTRNPFPYHPRQFSDYIYAEILATRLQEPEERAGYEALLKEM
                     EDRGADPSKLNVKWRVYHRTPGRVLSGTLTPKIKSLLDGSFFFGQTGFAIFRKGLSDN
                     AHAAIFRFGPSLVHGHLDDLNVNYFAQGYELTYDLGYSLGSTHTQVGWAKQTIAHNTV
                     VVDQQSQAGTQFGGSLLRFLDLPEMALAEATSNAYREIGVDTYRRLIVLTGDYLLDVF
                     RVKGGTTHDLPLHALSVDMSVEGLELGSVQKGSLAGQDFRWGHMQMNDGDMSGYPNKP
                     SWNPPPGNGYGFLVDVQSATPTQPWKAAWQIPDGSDTRLSFLPLYQASDTVFSATTPG
                     LYPHFPRARHIIRRRQGENLSSCFVSVWQAGTASYPDRVRSITRLDRGLETISADSAL
                     AVQLDLGDDIKDIWFLNPHSDTFVDATVDGQTARFKGSLARCRLQAGELTHLVMLDAS
                     EIAIGGWVLRLDQPARSASVASLPDGSPQLRIGPEWSDDARYDGCPVFFSNSSYTHQS
                     VYTVQQCRRRKMILQQSDTLLGRAVVHEILDASRMTTLVPHEYARHVLRKSPSGYFRG
                     KKLCNESRTVWTTVHSVKQGAPVVEIEVDSTTGFKAGDVFFYNDIQPGDTLTVQHFVE
                     LKKLPEGTWALNANTDVMVSVPDSFPAMAYTDASGAFKALDRGAVQRGSLPDSGETFL
                     RPLQ"
     gene            complement(2228..3034)
                     /locus_tag="PKH07_04575"
     CDS             complement(2228..3034)
                     /locus_tag="PKH07_04575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34254.1"
                     /translation="MALPENAEQTQSGGTYAVVRDRFEPWDFMRVSEVLEHCLGLHVL
                     DARQLARRAPGIVAEAIPGFQAERLAQNLRSVGTTTIIVPTRLLMPLPVEFQCRGMVF
                     QEGRLCFLGEDDRKSIALEPEAFTILCAAQVFREKEVGLSTTAKAFVYATMGYSRIRV
                     QDVPITSKGSKETWPFVLCLVGNNPPIQVRIYADKFNFSCLERHQVERTEDAFPHLVK
                     RLSECCPSVKKNPGVDALLQGKPLKKSLRFGKERDFEDYAHWFLQVSLLV"
     gene            3227..>3905
                     /locus_tag="PKH07_04580"
     CDS             3227..>3905
                     /locus_tag="PKH07_04580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34255.1"
                     /translation="MFYSGYLPVLFFVLCFGACSTQRDQGTRAQDKVASPEDIPSLAL
                     ITDRELFETQTEEKGSYVSSMVIASSGEIISVVSPSGAIKRYDSDFQSLGEISRPDPT
                     WIPFLVQITPDDILYLASGSSVQRFELKTNQWDQQFTTALPKMQKVYAIASDVTGDLW
                     VLRDNPDRTDKTRPKQVLHVTEEGVILNQWSVPSVAALANRMTCAPDGTILITETSSI
                     WWFSRSGS"
BASE COUNT          871 a         1035 c         1040 g          959 t
ORIGIN      
        1 accggtcgtc accagaaatc cctttcccta tcaccccagg cagttctctg actacatcta
       61 tgcggagatt cttgctacca ggctccagga acctgaagaa cgagcaggct atgaggcctt
      121 gctcaaagaa atggaagacc gtggtgcgga tccgtcgaag ttgaacgtca aatggcgtgt
      181 ctatcatcga acgccgggtc gggtcctctc tggcacactg actccgaaga tcaagagtct
      241 gttggatggt tcattcttct tcgggcagac aggtttcgcc attttccgaa aaggtttgtc
      301 ggacaatgcc catgctgcca tctttcgttt tgggccatcc ctggtccatg ggcatctgga
      361 cgatctcaac gtcaattatt tcgcgcaagg ctatgaactg acctatgacc ttggatacag
      421 cttgggcagt acgcatactc aagtgggttg ggcaaagcag accatcgctc acaatacggt
      481 cgttgttgat caacagagcc aagccggaac gcaattcggt ggatcgctcc tccgcttcct
      541 cgacctgcca gaaatggctc tggcggaggc gacatcgaac gcctatcggg aaatcggggt
      601 tgatacctac cgcaggctca tcgtgctgac tggcgactat cttctggatg tgtttcgagt
      661 caagggagga acaacccacg atcttcctct gcatgcgctc tccgttgata tgtcggtcga
      721 aggactggaa ctgggttcgg ttcagaaggg aagccttgcc ggtcaggact ttcggtgggg
      781 acacatgcag atgaatgatg gtgacatgag cggatatccc aacaagccct cttggaatcc
      841 gccacctggc aacgggtacg gattcctggt tgacgtgcag tcagcaacac ccacgcagcc
      901 ttggaaagcc gcttggcaga ttccagatgg gagcgacaca cgtttgtcct tcttgccgct
      961 gtatcaagcg tcagacacag ttttctcagc cacaacgccc ggcctctatc ctcatttccc
     1021 acgcgcaaga cacatcattc ggcgtcggca gggcgagaat ctatcgagtt gttttgtttc
     1081 tgtctggcag gcagggactg cgtcatatcc cgatcgagtt cgatccatca cccgtttgga
     1141 cagaggtctg gagacaattt cagcagattc ggcgctggcg gttcagctgg acttgggcga
     1201 tgacatcaag gacatttggt tcctgaatcc gcattcggat acctttgtgg acgcgacggt
     1261 tgatggacaa acggcaaggt tcaagggttc tctggcacga tgccgacttc aggcgggtga
     1321 gctgacacat cttgtcatgc ttgatgcctc cgagatcgcc attggcgggt gggtgttgcg
     1381 cctggatcag ccggcgcgaa gcgcatcggt tgcgagcctt cctgatggct caccccagct
     1441 gcgcatcggt cccgaatggt ctgatgacgc acgatatgac ggatgcccgg tgtttttcag
     1501 caacagttcc tatacccatc agtcggttta cactgtccaa caatgtcgca ggagaaaaat
     1561 gatcctgcag cagagcgata cgttacttgg gcgtgccgtg gtgcatgaga ttctcgatgc
     1621 ctcacgaatg accacgcttg ttccacacga gtacgctcga catgttctcc ggaaatcgcc
     1681 atcgggatac tttcgcggca agaaactgtg caatgagagc cgtactgtct ggacaacggt
     1741 tcattcagtc aagcaaggtg ctccagtcgt ggagatcgaa gttgactcga caacaggatt
     1801 caaggcgggc gatgtattct tttacaacga tattcaacca ggcgatacgc ttactgtgca
     1861 gcacttcgtc gagttgaaga agctgcctga aggaacatgg gcactcaatg cgaatacgga
     1921 tgtcatggtg tctgtgcctg attccttccc agccatggcg tataccgatg cgagtggtgc
     1981 tttcaaggct ttggatcggg gtgcggttca acgcggttca cttcccgact ccggcgagac
     2041 gttcctgagg ccgcttcagt gacagggcgc atggaattct ctggtgagca ggatagcacc
     2101 tgcaggtgcg tgagcgatgg cctctccctt ggatgctcgc tcgggaaatg acacgcctct
     2161 cttggactct gtgtcactgg gagaagtgtc ctgtcgccga ccgaagtaag gttgcgttca
     2221 aaacgattca gaccaacagc gagacctgaa gaaaccaatg ggcatagtcc tcaaaatcac
     2281 gttcctttcc aaagcggagt gattttttca gaggtttccc ttgaagcagt gcatccacgc
     2341 cgggattctt cttgacgctc gggcaacatt ccgagagcct tttcacgaga tgtggaaaag
     2401 cgtcttcagt ccgctcgact tgatggcgct ccaggcagga gaagttgaac ttgtccgcgt
     2461 agattctcac ctggattggt ggattgttgc cgaccagaca gagcacaaag ggccaggtct
     2521 ctttacttcc ctttgaagta atgggcacat cctgaacccg aattctgctg taacccattg
     2581 tggcgtagac gaaggccttt gcagtcgtag agagtccgac ttccttctct cgaaatacct
     2641 gagcggcgca gagtatcgtg aatgcctctg gctccagtgc aatgctcttt cggtcgtcct
     2701 cccccaaaaa acaaagtctg ccttcttgaa agaccattcc ccggcactga aattcaacgg
     2761 gcagtggcat gagcaggcga gttgggacaa tgattgtggt agttccgact gatcgtagat
     2821 tctgcgccaa gcgttctgcc tgaaagccgg gaattgcttc agcgacaata ccgggtgctc
     2881 ttcgagccag ttgtcttgca tcgagaacat gaagtcccag acagtgctcc agcacttctg
     2941 agacacgcat gaaatcccac ggttcaaatc ggtcacgcac aacggcgtat gttccccccg
     3001 actgagtctg ctctgcgttt tccggaagtg ccatgaccaa gtctccttgc gattcttcca
     3061 ggcagttaca tctggggttt gccctccgac agcacaaacg gtgctaccga tggtcgttga
     3121 catcattgaa cgagtctctg ctcttgtcaa caaaccttgt tgacaaccag tcccgtcaat
     3181 tgcaatactc tcagcgctta caatgatggg attgcataga aaaagagtgt tttattccgg
     3241 atatcttccg gtcttgtttt ttgtcttgtg ttttggcgca tgttccacgc aaagagacca
     3301 gggcacccga gcgcaagaca aggtcgcttc cccagaggat attccatccc tcgctctgat
     3361 cacggacaga gagttgtttg agactcagac tgaagagaag gggtcttatg tctcttcaat
     3421 ggtcattgcg tccagcggcg agattatatc cgttgtctct ccatcgggag cgattaagcg
     3481 atacgattcg gactttcaat ccctgggtga aatctcccga cctgatccaa cctggattcc
     3541 ttttcttgtc cagattacac cggacgacat tctttatctg gcgagtggca gttctgtcca
     3601 acgttttgag ctgaagacca accagtggga tcagcagttc acaacagctc ttccgaaaat
     3661 gcagaaagtc tatgccattg catctgacgt gacgggcgat ctctgggttc ttcgcgacaa
     3721 tccggatagg actgacaaga cgagacccaa gcaggttttg catgtcactg aagaaggcgt
     3781 gatactgaac cagtggtctg tcccatccgt tgccgctctt gccaacagga tgacatgtgc
     3841 tcctgatgga acgatcctca tcaccgagac ttcttcgatc tggtggttca gtcgttctgg
     3901 ctcgc
//
LOCUS       DAOQUC010000192         3858 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_26642_length_3858_cov_3.062845, whole genome shotgun sequence.
ACCESSION   DAOQUC010000192 DAOQUC010000000
VERSION     DAOQUC010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3858
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26642_length_3858_cov_3.062845"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2151)
                     /gene="mutS"
                     /locus_tag="PKH07_04585"
     CDS             complement(<1..2151)
                     /gene="mutS"
                     /locus_tag="PKH07_04585"
                     /inference="COORDINATES: protein motif:HMM:TIGR01070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HNT34256.1"
                     /translation="MSDDFSPLMLQYQRLKATCPDVILMMRVGDFYEMFNEDADVASR
                     ILDITLTKKSIGKGKTCPLAGIPYHALHNYIYKLTRSGYRVAICEQTEDPRKTKKLVR
                     REITRTVTPGTIIDSEVIDGTSNNYLAAIEDGGFYGLGLAFADVSTGEFRYTVLDGSD
                     AFSQLESELLKLSPSEVLLSQDIAADSSLRTLLQEHSNAVVTHRSPEAFDPSYLKELE
                     IQISQGTVAENEAVGLKAAGAVYQFVLETQRSTAPHIVRLEGYHPSSFMLLEANTERN
                     LELLVNIRDKGKAGTLLSVLDDTVTAVGSRLLKRWIVRPLLLPNIIKVRQDGVELLFR
                     NGFLRQDLRAALKNVPDLERLTSRVGFGNANARDLLALVNAAGAVCEINRLLQSHSDL
                     WPTWPSRWEVPLKGDQGTSAPTLLDELPELVALLAPVLSENPPLSIKDGGIFREGVNA
                     ELDELREIRRGGKDWIARLRDSERQRTGINSLKIGYTKVFGYYIEVTKPNLSLVPEDY
                     ERKQTVVNGERFVTRELKEYEAKVLGAEERIHDLEYELFMELLEKVKPFTERLLRVAG
                     SIAELDVLRSLAEVAVQNDYVRPEVDQKAEITLKECRHPVLEQSPVVPMFVPNDCSLD
                     DEKEQLIILTGPNMAGKSTFIRQVALNVLMAQMGSFVAAKWAKVGIVDRLFTRVGASD
                     NLSAGQSTFMVEMSETADILRKATPRSLIILDEIG"
     gene            complement(2148..3161)
                     /gene="ribF"
                     /locus_tag="PKH07_04590"
     CDS             complement(2148..3161)
                     /gene="ribF"
                     /locus_tag="PKH07_04590"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HNT34257.1"
                     /translation="MKVVDQSVFDGFSLGERDKSWIALGIFDGLHLGHGTVLGECVRC
                     AREREGTACVFTFASHPGGVLGRDPVQKALSTVEERLAGFERLGIDVALVPEFTLEFA
                     RKAPEEFVTEILVGRLHAQGVVVGFNYRFGRKKSGDAEMLVALGASHGFHVRVIAPSL
                     FQDQPISSTRIRQTLSEGQIEDAGRMLGYRFTLVGNVVPGAGRGKTIGFPTANLRLLR
                     PSFLPDGVYAARAWIKEAVQEQPSRPGMDAMLYIGNRPTYSADSIEKVVEIHVMGLSE
                     NLYGRLLGVQPIQKLRDDRRFDSEEELTRQIVSDIHSAKYLFSTQSNLAHPVTPTGTS
                     VKP"
     gene            complement(3282..>3858)
                     /locus_tag="PKH07_04595"
     CDS             complement(3282..>3858)
                     /locus_tag="PKH07_04595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34258.1"
                     /translation="VTDTFTFSFSDPGTVTLTATDLAEDEPVYLIVEPTVTPTETPTN
                     TPTITPTDTLTNTPTETPTQTPTFTPTRTPTKTPTKTRTPTPTDTPSDTPTVTPSRTP
                     TRTPTRTPTQTPSRTPTVTPTNTPDLWTLCNLVKDDRIDAKDILEFLRLKHLYEQGDR
                     THSMRADFNGDGKVDSDDLFLFLVVWSVVVP"
BASE COUNT          865 a         1069 c         1005 g          919 t
ORIGIN      
        1 accgatctca tcgaggatga tcagacttcg gggtgtggct ttccgaagga tatctgccgt
       61 ctcactcatc tcgaccataa aggtactctg acccgcggag aggttgtcag aggctcctac
      121 tcgtgtaaag agccgatcca cgattcctac ctttgcccac ttggccgcaa caaacgatcc
      181 catctgcgcc atcagaacat tcagtgccac ctgccgaatg aatgtggact tgcccgccat
      241 gttcgggccg gtgaggatga tcagctgctc cttctcgtcg tccagactgc agtcgttggg
      301 aacaaacatc ggcacgaccg gcgactgctc caacacggga tgccgacatt ccttgagtgt
      361 gatttctgcc ttctggtcca cctcgggtcg aacgtaatca ttctgaaccg ccacttcggc
      421 cagacttcgg agaacatcca actcggcgat ggaaccggcc actctcaaaa gcctctctgt
      481 aaagggtttg accttttcga ggagttccat gaacagttca tactccagat cgtggatgcg
      541 ttcctccgca ccgaggactt tggcttcata ttccttgagt tcgcgggtga cgaatcgttc
      601 gccattgacc accgtctgct ttcgttcata gtcttcaggg acaagactga gattgggctt
      661 ggtgacttcg atataatacc caaagacctt tgtgtaaccg atcttcagag agttgatacc
      721 cgtgcgttgg cgttcactgt cacgaagtct tgcaatccag tccttgcccc cgcgtcggat
      781 ttcacgcagc tcgtccagtt ccgcgttcac cccttcgcga aagatgcctc catccttgat
      841 agacaggggt gggttttctg acagaacggg agccagcaac gcaaccagtt cgggtagttc
      901 gtccaggagt gtgggcgcag aagtaccttg atctcctttc agaggaacct cccatcggga
      961 tggccatgtc ggccacagat cggaatgact ctggagcagt cggttgatct cacacaccgc
     1021 ccccgcggcg ttaaccagcg ccagcaagtc ccgcgcattc gcattgccaa atccgactcg
     1081 actggtcaat cgctccagat cgggaacatt cttgagtgcc gcacgaaggt cttgacgcag
     1141 gaatccattt cgaaaaagca gttccacgcc atcctgacga accttgatga tattgggcag
     1201 gagcagcggt cgcacaatcc aacgtttcaa cagacgactt cccactgcgg tcaccgtatc
     1261 atccaggaca gagagaaggg ttcctgcctt gcccttgtcc cgaatgttga ccagcagttc
     1321 gagattcctt tcagtgttgg cttcgagtag catgaacgag ctgggatggt aaccttcgag
     1381 tcggacgatg tgtggtgcgg tgctgcgctg tgtttcgaga acaaactggt agacagcccc
     1441 cgcggccttc agaccaacgg cttcattttc cgcaaccgtc ccctgagaga tctgaatctc
     1501 gagttctttg agataacttg gatcaaaagc ctctggagaa cgatgcgtga cgacagcatt
     1561 ggagtgttct tgcagcaatg ttcgaagtga agaatccgcc gcgatgtcct gactcaacag
     1621 cacttcgctg ggggagagct tgagcaactc actctccaac tgggagaaag catccgagcc
     1681 atccagaacc gtatagcgga actcgcccgt cgaaacatct gcgaacgcca acccgagacc
     1741 gtagaacccc ccatcctcga tagcggccag atagttgttc gatgtgccat caatgacttc
     1801 agagtctatg atggtgcctg gagtcaccgt tcgagtgatc tctcttcgaa cgagtttctt
     1861 ggtctttctt ggatcttccg tttgctcaca gatggcaact cggtatccac ttcgtgtgag
     1921 cttgtagata tagttgtgca atgcgtgata gggaataccg gcaagtggac acgtcttgcc
     1981 tttgccaatg gacttcttgg tcaacgtgat gtccagaatc cgagacgcaa catcggcatc
     2041 ctcattgaac atctcataga agtcgcccac ccgcatcatc aggatgacat cagggcaggt
     2101 cgcctttagc ctctggtact gcagcatgag tggggagaaa tcatcgctca tggtttcacc
     2161 gaagttccag tcggagtgac cgggtgcgcc aaattgcttt gggtgctgaa aagatatttc
     2221 gcactgtgaa tgtcgctgac aatctgtcgg gtcagttcct cttccgagtc aaagcgccga
     2281 tcatcccgca atttctggat gggttggaca ccaagcagtc gcccatacag attctcagaa
     2341 agacccatga catgaatttc gacaaccttc tcgatggaat cggcggaata ggttgggcga
     2401 ttgccaatgt acagcatggc gtccattcca ggtcgggacg gttgctcttg aacggcttcc
     2461 ttgatccagg ctcgggcagc gtagacacca tcgggaagga atgacggacg aagaagacga
     2521 aggtttgccg ttgggaatcc tatggtcttg cctcgtcctg caccagggac gacgtttcca
     2581 accagcgtga atctgtagcc caacatccgg ccggcatcct caatctgccc ttccgaaagc
     2641 gtctgccgaa ttcgggtact gcttatgggt tggtcttgaa agagactcgg agcgatcact
     2701 cgaacatgaa agccgtgaga cgctcccaaa gcaaccaaca tttcggcgtc tcccgatttt
     2761 ttccttccaa agcgatagtt gaaaccgacg actacgcctt gagcatgaag ccgccccacc
     2821 aaaatctcag tcacaaattc ctcgggagcc tttcgcgcaa actccagtgt aaactccggc
     2881 acgagcgcaa catcaatacc caaacgctca aatcctgcca gacgttcctc aacggtggag
     2941 agcgccttct gaaccggatc acgtcccaac acacccccag gatgactggc aaaagtgaat
     3001 acgcaggcgg ttccttcccg ctccctggcg catcggacgc attcgccaag caccgtgccg
     3061 tggcccaaat gcagtccgtc aaaaatcccc agagcgatcc aggacttgtc ccgttcgcca
     3121 agcgaaaacc catcgaaaac gctttggtca actaccttca tgtttgtggg gttgattcat
     3181 aacacaacac ccccaaacca gcaagcagac tgcactcatg cagtgcacag agtgtccctg
     3241 gcaatctgcc tcggtttcac tcaaaacagc gccactcctg actaagggac aacgactgac
     3301 cagaccacca agaacagaaa cagatcatcc gaatcaacct ttccgtctcc gttgaagtct
     3361 gctctcattg agtgtgttct gtcgccctgc tcataaagat gcttgagcct taggaattca
     3421 agtatatcct tcgcgtctat tctgtcgtct ttcacaaggt tacagagcgt ccaaagatcc
     3481 ggcgtattcg ttggcgtgac agtaggcgtt ctgctcggag tctgggttgg cgttctcgtc
     3541 ggggtccgag tcggagtcct gcttggtgtc actgtgggtg tatcggatgg tgtatccgtc
     3601 ggtgttggag ttcgagtctt ggtcggagtc ttggtcggag tgcgtgtggg agtaaacgtt
     3661 ggcgtctgcg tgggtgtctc ggtgggtgtg tttgttagtg tgtctgttgg agtgatggta
     3721 ggagtattgg tcggtgtctc tgtgggcgtc actgttggtt caacgatcag atagactggc
     3781 tcatcctcgg caaggtctgt tgcggtcaga gtcaccgttc ccgggtcgct gaaactgaag
     3841 gtaaatgtat ccgtcacc
//
LOCUS       DAOQUC010000193         3838 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_26849_length_3838_cov_2.970130, whole genome shotgun sequence.
ACCESSION   DAOQUC010000193 DAOQUC010000000
VERSION     DAOQUC010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3838
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26849_length_3838_cov_2.970130"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..940
                     /locus_tag="PKH07_04600"
     CDS             <1..940
                     /locus_tag="PKH07_04600"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34259.1"
                     /translation="APAPEGVKQVLIEVKVWETSHRNQQQVDFDWTWLNLDRDGSPAG
                     GALKGGHINFNPKPSVGLSPLDVDVLRSSYGQLRMQLRAMSARGEAQVLATPVILTMD
                     LQPAEIKTTDKIPIVQMDSGRLKTSSRDSGVFLKVTPEIMGEYVQMTVNASVSLITRY
                     VRQADPSRENRIYEQPVIAERHVDTKLRVHSGEGIFLGGLVEEIQQQTVKRVPIISRF
                     PWLPEATKGVPGLNALWLVGRLFENRDTDLVRKELFIEIVPRILEPGEKVYWPTQILP
                     EDRPRILDLYNMGLSNLLPQDEMIQETPQQTQQNAQ"
     gene            930..1811
                     /locus_tag="PKH07_04605"
     CDS             930..1811
                     /locus_tag="PKH07_04605"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HNT34260.1"
                     /translation="MLSEFVPTVSLNQGERSKGFYMVFSNGLFRIFQVVALLLVISSS
                     GFCQSAMDTTRVLTLPSKFDSPANSDCPFPSGEKLSFDISCLISKVGSMELTFWGLVD
                     HEGDDVLRVTTYSQGLGMKDWEEILACPEEWLPLLVRREVFKAGLKTRVIENYDQDAF
                     RVVVAPEGNPQQPTEIIDSDGRMQNVILLYYKFRMMQLQSGQNYHINLPRESFQVRVG
                     DVETVSVPYGDYQARPLVSDPKKFTVWITSDGNPLPAKMVLKTNLGTYTFSLTGREVI
                     VPKTAQSEPPSTLNNIQ"
     gene            complement(1753..2646)
                     /gene="ispE"
                     /locus_tag="PKH07_04610"
     CDS             complement(1753..2646)
                     /gene="ispE"
                     /locus_tag="PKH07_04610"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HNT34261.1"
                     /translation="MMTQHNDLQCWRRSAHAKLNLFLSVQNKRADGFHDILAVNQEIS
                     LSDSLVFTPEPARFTVSVQGNPDVPSDERNLVYKAAKALFADDALPKMRVCVEKTIPT
                     GAGLGGGSSDAAATLSFFNSHFGINKPLDELIAIAKSVGSDVPFALMGGTALVSGVGE
                     KIQRLPEPQEPVWFVVAAPTVSVSTGHAYRWIDEQQDRTPAPQPDQLIHSLLDRDLES
                     LARHLHNDFEQVILKRHPQIGNLKQALLDAGALGALMTGSGSNVFAVVRSQDHAELVK
                     RRITGCCSACWVAHSVPFSAR"
     gene            complement(2627..3343)
                     /gene="ispD"
                     /locus_tag="PKH07_04615"
     CDS             complement(2627..3343)
                     /gene="ispD"
                     /locus_tag="PKH07_04615"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HNT34262.1"
                     /translation="MKTVPRTAAVVVAAGVGRRMRQDKVLLRLRDKPILLYSLHALAD
                     HPGIDRIVIVTRSEIMPQVQSELISLPAKHAQSSVIEGGQQRYDSVWNAIQHLQHNDP
                     PDIVLIQDGARPFLSQKLISQSIEAASEFGAACVAIKAEDTLKRSLDECTIEETLDRS
                     TVWRAQTPQTFRFSLLFECFERLRHEGLLTDVTDDASVLERCGHPVRIVQGDTRNIKI
                     THPLDLAIAEALLNNDDTTQ"
     gene            complement(3746..>3838)
                     /locus_tag="PKH07_04620"
     CDS             complement(3746..>3838)
                     /locus_tag="PKH07_04620"
                     /inference="COORDINATES: protein motif:HMM:NF013266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bS18 family ribosomal protein"
                     /protein_id="HNT34263.1"
                     /translation="TGTCASHQRMLTLAIKRARFIGLLSYIRKP"
BASE COUNT          854 a          985 c         1062 g          937 t
ORIGIN      
        1 agcacccgct ccggagggcg tcaagcaggt gctcattgaa gtgaaggtct gggagacctc
       61 acatcgcaac cagcaacagg tggatttcga ttggacatgg ttgaacctgg accgtgatgg
      121 cagccctgcc ggtggtgccc tgaaaggcgg gcacatcaac ttcaacccga agccttcggt
      181 aggtctttcg ccgttggatg tggatgtgct tcgttcatct tacggtcaac tgcgaatgca
      241 gttgcgagcc atgtctgccc gtggtgaggc gcaggtcttg gcgactccgg tcatcctgac
      301 catggatctt cagccagccg aaatcaagac cacggacaag attcccatcg ttcagatgga
      361 cagtggtcgc ctgaagacct cttcgcggga ttcgggagtg tttctgaaag tgactcctga
      421 gatcatgggt gagtacgttc agatgactgt caacgcgagc gtgagtctca tcactcgtta
      481 cgttcgacag gcagatccga gccgtgaaaa ccggatctac gaacaacctg tcatcgctga
      541 gcgccatgtt gacacaaaac tgagggttca ttccggggaa ggcatcttcc tgggtggact
      601 ggtggaagag attcagcagc agactgtcaa gcgcgttccg atcatcagca gatttccttg
      661 gctgcccgaa gccacaaaag gcgttcctgg tctgaacgct ctgtggctgg ttggaaggct
      721 ttttgagaat cgagataccg atcttgttcg caaagagctg ttcattgaga ttgtacccag
      781 aatccttgag cccggcgaga aggtctactg gccgacgcag atactgcctg aagatagacc
      841 gaggattctg gatctttaca acatggggct gtcgaacttg cttccccagg atgagatgat
      901 tcaggagaca ccacagcaga cgcagcagaa tgctcagtga gtttgtcccg accgtttcgc
      961 tgaaccaagg agaacggtca aagggatttt atatggtctt ctcgaatgga ctgtttcgga
     1021 tctttcaggt tgtcgccctc ttgttggtca tttcgtcttc tggattttgt cagtctgcca
     1081 tggacaccac gagagttctg acgctcccct ccaagtttga ttctcctgcc aactccgact
     1141 gtccctttcc cagcggagag aaactcagtt ttgacatctc ctgtctgatc tcgaaagtgg
     1201 ggagcatgga actcactttc tggggacttg tcgatcatga aggtgacgat gtcctgcggg
     1261 tgacgaccta cagtcaggga ctggggatga aggactggga agaaattctt gcctgtcccg
     1321 aggaatggct tccgttgttg gtgcggcgtg aggtattcaa ggccggcctc aagacgcggg
     1381 tcatcgagaa ctacgatcag gacgcgtttc gcgttgtcgt tgctccagag ggcaatccgc
     1441 agcaaccgac ggagatcatt gatagtgatg gcaggatgca gaacgtgatt ctcctttact
     1501 acaagtttcg catgatgcaa ctgcagtcgg gtcagaacta tcacatcaac cttcctcgag
     1561 agtcattcca agtgagagtg ggtgatgtgg agacggtttc cgttccgtac ggtgactatc
     1621 aagccagacc tttagtcagc gatccgaaga aatttactgt ttggatcacc tcagacggga
     1681 atccgcttcc ggcgaagatg gttctgaaga caaacttggg aacttatact ttttcgctga
     1741 cggggcgtga ggttatcgtg ccgaaaacgg cacagagtga gccacccagc acgctgaaca
     1801 acatccagtg attcttcttt ttaccagttc ggcatggtct tgagaacgga caaccgcgaa
     1861 gacgtttgat cccgaaccgg tcatcaaagc ccccagcgca ccagcatcaa gtagagcctg
     1921 cttgagattc cctatctgcg gatggcgttt caggataacc tgttcgaaat cattatgaag
     1981 atgtctcgcg agagactcca aatccctatc gagaagagaa tgaatcagct ggtcaggttg
     2041 tggcgctggc gttctgtcct gttgctcatc gatccaacgg tatgcgtggc cggtgctgac
     2101 agataccgtg ggtgcagcaa ccacaaacca gacaggttcc tgtggctccg gaagccgctg
     2161 gatcttctct ccaacacctg aaaccagtgc ggttcctccc atcaaggcga aagggacatc
     2221 agagccgact gacttggcga tggcaatcag ttcatcgagt ggtttgttga taccaaaatg
     2281 ggagttgaaa aaactcaagg ttgccgctgc gtctgaactg ccacccccca agcccgcgcc
     2341 agttggaatc gtcttctcaa cgcagacgcg catcttgggc agggcatcat cagcaaagag
     2401 tgcctttgcc gctttgtaga caagattcct ctcgtcgctg ggaacgtctg gattgccttg
     2461 aacggaaaca gtgaatctcg cgggctccgg tgtgaacaca agtgagtcgg aaaggctgat
     2521 ttcctgattc acagcaagaa tatcgtggaa cccatcggca cgcttgttct gaacgctgag
     2581 aaacaagttc aactttgcat gggctgatct gcgccagcat tgcaggtcat tgtgttgtgt
     2641 catcattgtt caacaatgcc tcggcgattg ccaagtcgag agggtgggtt atcttgatat
     2701 tgcgtgtatc gccctggaca atccttaccg gatgaccaca gcgctccaga actgaggcgt
     2761 catcggtcac atcagtcaga agcccctcat gtctgaggcg ttcaaagcat tcaaacaaaa
     2821 gggagaagcg aaatgtttgc ggtgtctgcg cccgccaaac cgttgagcga tcaagagtct
     2881 cctcaatggt gcattcatcc agagatctct tgagggtatc ctccgccttt atcgcaacac
     2941 acgctgcgcc gaattcagag gcggcttcaa tgctctgtga gatgagtttc tgactcagaa
     3001 agggtcgggc accgtcctgg ataagcacga tgtccggggg atcattgtgc tgcagatgct
     3061 gaattgcgtt ccatacggaa tcgtatcgtt gctgtcctcc ctcgatgacc gagctctgtg
     3121 cgtgtttcgc ggggagcgag attagctcac tctgcacctg gggcattatc tccgagcgtg
     3181 tgacaatgac aatgcgatca attcccggat ggtctgccaa cgcgtgcagc gagtacagaa
     3241 gaattggttt gtcacgcagg cgaaggagaa ctttgtcttg tctcatccgc ctgccgacac
     3301 ccgctgccac gacgactgct gctgttcgag gaacggtttt catcgtctca ctcaagagag
     3361 gactgcaccc tatgcgcttg cgatacgatt caggagtctc gcaattctgg actttttccg
     3421 ggctgcatta ttggggtgaa tgattctccg cccagcggct tggtcaatga gccgacaggt
     3481 ctccttagcg cgctgctgga gagtctcccc tttcagtgtc cccttttctg attccgtcac
     3541 gagggtcttc accagggtgc ggattcggct tttgttggac ttcacgcgaa gtctgcgaag
     3601 cttatcttgg cgcaaccttt tccagttgga cttgtgtgta ggcatccttt tgagtcacct
     3661 ccttggattt gtccttggca ctgagcacct tcccatgggc ggccacccga gtcggatgtg
     3721 cctctggtga aggtgctgtc cgagatcagg gcttccttat ataggaaagc agaccgatga
     3781 atcgcgcacg cttgatggcc aaagtaagca tcctctgatg tgaggcgcat gtgccagt
//
LOCUS       DAOQUC010000194         3837 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_26856_length_3837_cov_3.774987, whole genome shotgun sequence.
ACCESSION   DAOQUC010000194 DAOQUC010000000
VERSION     DAOQUC010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3837
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26856_length_3837_cov_3.774987"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..887
                     /locus_tag="PKH07_04625"
     CDS             <1..887
                     /locus_tag="PKH07_04625"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNT34264.1"
                     /translation="YGSHREMLEKEQLDVVSVCTPNYAHKEGTIDSLKAGCHVLCEKP
                     LAVTAKDVREMFAEAKKRRKVLMTAQHFRFTSEARIAHEFVKAGELGDVYFGKVFAIR
                     RWGIPGWGVFHIKDKSGGGALIDIGVHQLDLTLWLMGSPEPVTVSGMTYAKVGKRKDL
                     SVRYGWNWDRNEFDVDDYAAGFVRFKNGACLTIECSWAGNIKQDRFETHIVGTEGGAS
                     LGPLQIHKVMHGALVDVTPINLPGLKPHHEEIRHFLNVLLGKEELIVKPHETLRVIQI
                     LEGLYRSSLAGKEIKVNY"
     gene            1186..1482
                     /locus_tag="PKH07_04630"
     CDS             1186..1482
                     /locus_tag="PKH07_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013466819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HNT34265.1"
                     /translation="MMLKTKEEILSALRDRQFELEDRFKVRKLALFGSYARGDQGTQS
                     DVDILVEVSPSIGLDFVTLAETIEQILGLPVDLVSERALKPRARRAIEAEMIYV"
     gene            complement(1574..2140)
                     /locus_tag="PKH07_04635"
     CDS             complement(1574..2140)
                     /locus_tag="PKH07_04635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34266.1"
                     /translation="MSEPTLREKLLDLESRESKLDQRTSDFMENIMYRKLNVPMKISF
                     SISLVMGVCFCVGFTYVGLTAPDELPPIFKLGFYVAGAFGLAWAVFSGYVLKKGRAHL
                     LKTDDIIQGLSFGFIVILMVVLLLASGYMKDDLTAVKMVLNGMVFLILFGIPAMLHMR
                     MRQTERMLMDRLLQIELKLTEMQEEKES"
     gene            complement(2133..2699)
                     /locus_tag="PKH07_04640"
     CDS             complement(2133..2699)
                     /locus_tag="PKH07_04640"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNT34267.1"
                     /translation="MSTQSNRIRTQLLVLRCQSGDENSFRELIQLWEKPLLYYIRRIV
                     LTQEDAWDVLQEVWINVVSKVRQIRNPACASVWLYRIARNTAFNSWRQKMKTEPLDEK
                     MENQLHDDSDPMDDLVSAEDAEAIHRALGQVPLPQREALTLFFLEDLSLQEISELTEV
                     PVGTVKSRLHHGRNKMRELLREEDFGNV"
     gene            2918..3748
                     /locus_tag="PKH07_04645"
     CDS             2918..3748
                     /locus_tag="PKH07_04645"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HNT34268.1"
                     /translation="MPRFKKGSLFIEAVVLFIAVLVLLPIALIFLTGFKADSEIIHFK
                     SLLPQQWTLENFREILENSEEIPIFQWLLNSLFISSMVTLLVLTVDSLAAYALARLDL
                     PGKKWVFPIIVGTLMVPGQILLVPVYLILNKIGWLDTPWALIVPAGAGAFGVFLLHQF
                     FLGIPRDLEDAAAIDGCSRLGTLWHVVLPLSKPALATLGIFVFVGSWNDFLGPLVFLD
                     SADKYTLPVGIALFQTSYYAEYGLTLAASMICTLPVLIVFLLFQKHIIKGISLTGLKE
                     "
BASE COUNT          861 a          988 c         1012 g          976 t
ORIGIN      
        1 ggtacggctc gcatcgcgag atgctggaga aggaacagct cgatgtcgtc agcgtgtgca
       61 ctcccaatta tgcgcacaag gaaggaacca ttgactctct gaaagcgggc tgccacgttc
      121 tctgcgaaaa gcctctggcg gtgacagcga aggacgttcg agagatgttt gccgaggcga
      181 agaaacgccg aaaagtcttg atgaccgcac agcactttcg attcaccagc gaagccagaa
      241 tcgcccatga gtttgtcaag gcaggggaac tcggcgatgt ctacttcgga aaggtgtttg
      301 ccatccgacg ttgggggatt ccgggctggg gtgtgttcca catcaaggac aagtccggtg
      361 gcggtgcgct cattgacatt ggggtacatc aactggattt gacgctttgg ttgatgggtt
      421 ctcccgagcc ggtgaccgtt tctggcatga cctatgccaa ggtgggcaag cgcaaggatc
      481 tctctgttcg ttacggctgg aactgggatc gaaatgagtt cgacgtggac gactacgccg
      541 cgggttttgt gcgtttcaag aacggcgctt gtctgaccat cgaatgcagc tgggcaggca
      601 atatcaagca agaccgtttc gagacgcaca ttgtcggaac cgaaggtgga gccagccttg
      661 gacccctaca gattcacaag gtcatgcacg gtgctttggt ggacgtgaca ccgatcaatc
      721 ttcccggctt gaaaccgcat cacgaagaaa ttcgacattt tctcaacgtg cttctcggca
      781 aggaagaact gatcgtcaaa ccgcatgaga cacttcgagt catccagatt ctggaagggt
      841 tgtatcggtc gtcgcttgct ggcaaagaga ttaaagtcaa ctactgacac acagagggtt
      901 gggggtgaag ccaacttcac ccccgaatca ctctgagcgg agtgagttgt aaggattctc
      961 tgcagatacc tcaccgacag agtcctgtct cggtatgata cttcgagccg catgcttgtc
     1021 attctggacg gagtgaggaa tctctcttcg ggagctgtag ccggcattct cgccatgcgg
     1081 agtctgagat ttggcggagt gagagtccgg ttttcacact gtcttgccgg cgtctcttga
     1141 cttctgtatg atagcaacat tgaatgttcc atagatagag gttgaatgat gctcaagacc
     1201 aaggaagaga ttctgagcgc attgcgagac agacagttcg aactggaaga ccgattcaaa
     1261 gtcaggaaac tggcattgtt cggttcctac gcgcgcggag atcagggcac acaaagcgac
     1321 gtggacatcc tggtcgaagt gtctccctca ataggtttgg acttcgtcac tctcgccgaa
     1381 acaatagagc agatccttgg tcttcccgtc gatctagtgt ccgaaagagc gctgaaaccg
     1441 agggcaagaa gagccatcga agcggagatg atctatgtct gaacggagtg atccttcttt
     1501 ttaacacttt cctgcaagac atgcgtgatt actatgggag accgttgccg gcgcttgttc
     1561 actccaaaca gttctaactt tccttctctt cctgcatttc cgtcagcttc agttcgatct
     1621 ggagcagtct gtccatcagc attctttcgg tctgtcgcat tctcatgtgc agcatcgctg
     1681 gaataccaaa gaggatcaga aagaccatcc cgttgagaac cattttcacc gccgtcaagt
     1741 catctttcat gtaaccgctg gcaagaagaa gaacgaccat caggatgaca atgaaaccga
     1801 acgacagccc ttggataata tcatcagttt tcaacagatg ggctcttccc tttttgagta
     1861 catagccgga aaacactgcc caagccaatc caaaagcgcc tgctacgtag aagcccagtt
     1921 tgaaaatggg tggcaactca tccggagcgg tcagccccac ataagtgaag ccaacacaga
     1981 aacacactcc catcaccaaa gaaatggaaa aactgatctt catcggaacg ttgagttttc
     2041 tatacatgat attctccata aaatcgctcg ttcgttggtc gagctttgat tccctggatt
     2101 ccaaatccag caacttctct cggagggtgg gttcagacat tgccgaaatc ctcctctctc
     2161 agtagctccc gcatcttgtt tctgccgtga tgcagcctcg atttcactgt gccgacaggg
     2221 acttccgtca gctcgctgat ctcctgaagc gacagatctt caaggaagaa gagcgtgagt
     2281 gcctctcgct gaggcagagg cacttgtccc aaagccctat ggatggcctc tgcgtcctcg
     2341 gcagagacga gatcgtccat cgggtcggaa tcgtcgtgca gttggttctc catcttctcg
     2401 tcaagcggtt cggtcttcat cttctggcgc caggagttga atgccgtgtt gcgtgcgatc
     2461 cgatagagcc aaactgaggc gcaggcggga ttgcgaatct gccgtacctt tgaaacaacg
     2521 ttgatccaca cttcctgaag cacatcccaa gcgtcctctt gcgtcagaac gattctgcgg
     2581 atgtagtaca gaagaggctt ttcccaaagc tggatcagtt cccgaaagga gttttcatcc
     2641 ccgctctgac agcggagaac aagcagttgg gttcgaatcc tgttgctctg tgtactcaaa
     2701 cgctcgactc actttcatcc gtacactaca tcagacacaa gaacttgtcc ccaggttcaa
     2761 atcaggtcgg agattctgac tcggagtttc catcttgggt cgctaccgta gcctgttgac
     2821 aaaaggctgc cttccgtctt aactacaaat acatgaacag ccgtaggggc agtgatcgct
     2881 gtgcctctcg tgtttacctc tgtgtaggag aacaagcgtg cctcgtttca aaaagggctc
     2941 tcttttcatc gaagcggttg tgttgttcat cgcggtattg gttctgctcc ccatcgcgct
     3001 gattttcctc acgggattca aggccgattc tgagatcatc cacttcaaga gcctcttgcc
     3061 gcagcagtgg acgctcgaga atttcaggga gattcttgag aactctgagg agattcccat
     3121 ctttcagtgg ctcctcaaca gtctgttcat ctcttcgatg gtgacgctct tggtgctgac
     3181 ggtggattcc ctggcagcgt atgcactggc tcgacttgat ctgccaggca agaagtgggt
     3241 gttcccgatc atcgttggaa cgttgatggt tccgggacag attcttttgg ttcccgtgta
     3301 tctcattctg aacaagatcg ggtggttgga cacgccgtgg gcgctcattg ttcctgcggg
     3361 tgctggtgcg tttggtgtct ttctccttca ccagttcttt cttggcattc cacgcgatct
     3421 ggaagatgcc gcagctattg acggctgctc tcgactggga acgctctggc atgtggtttt
     3481 gcctctatcc aagcccgcgt tggcgacctt ggggatcttt gtttttgtcg gctcctggaa
     3541 tgactttctg gggcctctgg tatttctgga ttccgctgac aagtacacgc tgcccgtcgg
     3601 cattgcgctg tttcagacgt cctactatgc cgaatacggg ctgactcttg ctgccagcat
     3661 gatctgcaca cttcccgttc tcatcgtgtt tctgcttttc cagaaacaca tcatcaaggg
     3721 tatttcactg acaggactca aagaatagat ccgagtgtgc tctgtcttgg ctcagttacg
     3781 gaggggggca ggcgaacacc cgtgtaagtg agatgtcact ctgagcggag tctgcgc
//
LOCUS       DAOQUC010000195         3836 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_26871_length_3836_cov_2.475536, whole genome shotgun sequence.
ACCESSION   DAOQUC010000195 DAOQUC010000000
VERSION     DAOQUC010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3836
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26871_length_3836_cov_2.475536"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..863
                     /locus_tag="PKH07_04650"
     CDS             <1..863
                     /locus_tag="PKH07_04650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34269.1"
                     /translation="TPFEGSIVTQYEPSQRGHLSVQLFSQLPAGAPIYVDDVFVEVVA
                     SQEAEVPASTWPVNCVRLAEGQEIVVDGNVSAQEYSGAQALVLNADALDDEDPYFPGV
                     VHQGTVVVGPAEYPVEDYSGTFYFMWDDAYLYAAISALDDAVSPATDAPNGADCLQFV
                     LGPVDGVDTAGMLIPTIAPDDGAGHPKAMNVFSGWLAKDLFAADTGTQYAAAVDAATN
                     DWTVEVRIPWTALQGSFTPELFPPAVGDQVGFSLLGIDYDNSVLEWFGCIENAPWTGQ
                     GLQTMTFIQP"
     gene            complement(1484..2755)
                     /locus_tag="PKH07_04655"
     CDS             complement(1484..2755)
                     /locus_tag="PKH07_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34270.1"
                     /translation="MQPNSLGKGHAMGLSKEHIKERIGSSLEHPYHGELAVGTMEQFR
                     KGVGDFHQWASGVISGPRPWLIDRLATIMSSLSLKTLDIDRARNYDWQESDLEGFCYR
                     IQALLPDGTLAGYYLGRIDSDTYAVFGYGIMVDEVASPSSSHAEAPSSRSSGMANPAR
                     ESSDFYSLLMDMMCCIMCADANMSKQEIGTACRILKELHVPWGRGEVERRLKEFVQRV
                     RSEGLLCVIEQTLDRLRDFGCLDRSKLMLDSIDRIVHADGRVDPRAMNVRQKFVDAFR
                     SVPEKPQDFSIDTEVGSWAQDDRLHRVERPVPRARSTLERKPSQARPGASRGSIPTAR
                     EIVDRRVAMAREFVLPQSRSKRDQVLEVMDRCLIDPDKMFTCLKQIGTRAEVLHMVNE
                     IERDLEAGRLDRSYIVISAAAKTIRQYSHLL"
     gene            complement(3280..3792)
                     /locus_tag="PKH07_04660"
     CDS             complement(3280..3792)
                     /locus_tag="PKH07_04660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34271.1"
                     /translation="MSIADLFRKKTEEEGDPVAREVKRHLEAMHKYFQLSPHYAATAP
                     HWRRLEKPEVIEWLGKNLREAMDQRGPVTQTLHLPDVLLIVTVIQAVQPQAIRAAFAP
                     SGYLTYVEKLGFFEAPRNRRAAAAFVLGHEHTALRVDLTWVPPLDNRPYQVIFAQDIL
                     TPQERQEIGI"
BASE COUNT          779 a         1180 c         1070 g          807 t
ORIGIN      
        1 ggacgccttt cgagggcagc atcgtcacac agtatgagcc ctcgcagcgt ggtcacctgt
       61 ccgtgcaact gttctcccaa ctgcctgcgg gcgcgccgat ctacgtggac gatgtcttcg
      121 tcgaagtcgt ggcgtcccag gaggccgagg tccccgcaag cacctggcca gtgaattgcg
      181 tcaggctggc cgagggtcag gagatcgtcg tcgacggtaa cgtgtccgcc caggagtaca
      241 gcggcgccca ggcgctcgtg ctcaatgccg acgcactgga cgatgaagat ccctacttcc
      301 cgggtgtcgt ccaccagggg accgtcgtcg tggggccggc ggagtacccg gtggaggact
      361 actcgggaac gttctacttc atgtgggacg atgcctatct ctatgccgcg atttcggccc
      421 tggacgacgc cgtgagcccg gcgacggacg cgcccaacgg cgcggactgc ctgcaattcg
      481 tgttggggcc ggttgatggc gtggacaccg caggcatgct cattcccacc attgcgccgg
      541 atgacggcgc gggacatccg aaggcgatga acgtcttcag cggctggctc gccaaggatc
      601 tcttcgccgc agacaccgga acgcagtacg ccgctgccgt ggacgcagcg accaatgact
      661 ggacggtcga agtccggatt ccctggaccg ccctgcaagg cagtttcacg ccggagttgt
      721 tcccgcccgc ggtgggtgat caggtcgggt tttcgctgct gggtatcgac tacgacaaca
      781 gtgtcctcga atggttcggc tgcatcgaga acgccccttg gacaggccag ggacttcaga
      841 cgatgacgtt cattcaacca tgacatgatg tggtctatag gtcgtgggtg acagaggccc
      901 aatcatgatg gcctctgtca ccctgtatcg gcaaagaact ccggatggcc atcgactctc
      961 tcaaggaaca aattggtgtc ctgagggaac accgggaagg aagagatgca tcctgctcga
     1021 taaccatccc cactcactga cgaggtcctt gacgaactgt cctctcaatc ccatgctctc
     1081 gtgttcggct ccggtattga ccgagggagg ctcgatcgga tcggcataga gatcctcccg
     1141 gttcgggtcc gttggaatcg gattgctcgc ccaacaatat cgatgtcggc ggcccggatt
     1201 gcgccgccgg cgcgcagcgc cgaccaacac ctctcgaccg ctcccgcaac ttcggacaca
     1261 atgccgccag atgtcccggt tcgaaaccat ccggcaggca caaatgctgc gaccgctgca
     1321 ggtcttgttg tcgtcatcat tgcaggctct gcttttgagt gcacatccgg gatctccaga
     1381 cgacgggcca agcgtcgcgc ttcttctgcc gggaaggcat tggacaacga tgcgatcgcg
     1441 taggcacccg cctggcatgc acagtgtgtc gcgagctgcc tgtctacagc aggtgcgagt
     1501 attgacgaat cgttttggct gcggcgctga tcactatgta cgatcgatcc aaccttccgg
     1561 cttccaggtc gcgttcgatc tcattcacca tgtggagcac ttcagctctg gtgccgatct
     1621 gcttcaagca ggtgaacatc ttgtccgggt cgatgagaca gcgatccatc acttccagaa
     1681 cctgatctcg tttggagcgt gattgaggaa ggacaaactc tctggccatt gcgactcggc
     1741 ggtcaacaat ctcacgtgcc gtcggaatgg atcctctgct tgctcctggg cgcgcctgcg
     1801 agggtttgcg ttcaagggtg ctgcgagctc tcggcacggg ccgctcaaca cggtgcaatc
     1861 tgtcgtcctg cgcccaagaa cccacttcag tgtcaatgct gaaatcctgc ggcttctccg
     1921 gaacgcttcg aaaagcgtct acgaacttct gacggacgtt catcgcgcgc ggatcgacac
     1981 gtccatcggc gtgaacgatc cggtcaatgg aatcgagcat caacttgctt ctgtcgagac
     2041 aaccgaaatc tcggagccga tcaagagtct gttcaatgac acacagaaga ccctctgacc
     2101 ggactctctg aacaaactcc ttgagccttc tctcaacctc accgcgcccc cagggaacgt
     2161 gcaactcttt cagaatacgg caggccgtcc ctatctcttg cttgctcatg ttggcgtcgg
     2221 cgcacatgat gcaacacatc atgtccatga gtaagctgta gaaatcggaa gactctcttg
     2281 cagggttcgc catgcctgaa cttcgtgacg acggcgcctc cgcatgcgat gaggatggag
     2341 acgcgacttc atcgaccatt attccatacc caaaaaccgc ataagtgtcg gagtcgatcc
     2401 gcccgagata ataacctgcc agagtaccat caggcaggag tgcctgaatc cggtagcaga
     2461 aaccctcaag gtcgctctcc tgccaatcgt agttcctggc acgatcgata tccagggtct
     2521 tgagggacag actcgacatg atggtcgcaa ggcggtctat gagccaaggc ctcggaccac
     2581 tgatcacgcc agaggcccac tggtggaagt cgccgactcc tttcctgaat tgctccattg
     2641 tccctacggc caattcgcca tgatatggat gctccaaaga actcccaatg cgttccttga
     2701 tgtgttcctt actcagaccc atcgcatgtc ccttccccaa actgttgggc tgcatagtga
     2761 atgaaggaat cctctcggca ggacaggact gttcctgatc cggcatggcc cggataggca
     2821 attagccgac atggtcggac gtggtgccga cggtgttgga gccgaggctc tctccatgtg
     2881 ttgcgggcga ccgagtaggg gtccatccca tcgcaatgcg accatcattc ccggcacgtg
     2941 agcaacctcc ttctgacgag tgacaatccg agactgccat ctctacctta tgactcgcct
     3001 ataccattct ggatggttcc acgggtccct gccctatgag ccatatagcc ttccgtgcaa
     3061 agccacacac ggcgttacac gattcgcctg acatcattgt actactgatc cggttcgtag
     3121 acaattgaaa gagggcgatg cctcgcccct gaaccagcaa cccgcagatc ctcagtctgt
     3181 tgcccaaccc ccgcaagtgc agcagccgcc gcaactcacg gactctcccg aagcaggtgg
     3241 ggcgccggcg gcgcgaaccc ggccctccaa gcgggtcacc taaatgccta tttcttgacg
     3301 ctcctgaggt gtcaggatgt cctgtgcgaa gataacctga tagggtcggt tatccagagg
     3361 aggcacccag gtgaggtcca cgcggagcgc cgtgtgttca tggcccaaaa cgaacgcggc
     3421 cgccgcacgg cgattccgag gtgcctcgaa aaaccccaac ttctccacat atgtcaagta
     3481 acccgacggc gcgaatgcgg cccgaatggc ctggggctgc accgcctgga tgacggtcac
     3541 gatcaacagg acatcgggaa ggtgcagcgt ttgggttaca gggcccctct ggtccatcgc
     3601 ttctcgcaag ttctttccca gccattcgat cacttccggc ttctccaggc gccgccagtg
     3661 aggggcagtg gcggcataat gaggcgacag ttgaaagtac ttgtgcatcg cctccaaatg
     3721 ccgtttcacc tcccgggcta ccggatcccc ttcttcttca gtcttcttcc tgaatagatc
     3781 ggcgatactc atgggtcgat ttctcctaca ttatcccatc tcttagacga gcggtc
//
LOCUS       DAOQUC010000196         3834 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_26888_length_3834_cov_3.174120, whole genome shotgun sequence.
ACCESSION   DAOQUC010000196 DAOQUC010000000
VERSION     DAOQUC010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3834
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26888_length_3834_cov_3.174120"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /locus_tag="PKH07_04665"
     CDS             complement(<1..440)
                     /locus_tag="PKH07_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007562784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HNT34272.1"
                     /translation="MRYFVFSVLVAVVVVLSCWAASDSAAQEEFRALWVTRFEWPDRN
                     PEVSKGKILSILDECQKANFNAVMFQCRGQADTLYPSPIEPWSPVFGSADPGWDPLEF
                     AIQEAHKRGLELHAYINPVPAWSADTPPPSTNPPHMYAQHGPGS"
     gene            complement(650..1576)
                     /locus_tag="PKH07_04670"
     CDS             complement(650..1576)
                     /locus_tag="PKH07_04670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34273.1"
                     /translation="MVDQDKISLISDVMPTQVKQRLFRVYFVAPTPSVASALASELCQ
                     DGLLVRSASSVRELAPESFTLPQAFAEPPDVIFLEQPRTEARAVEEIRRIQCHPALGT
                     AALVMVSEDRHYSLLMSSIACGATDYLLLPISKPRLVMQLNRLLGLERFGPMYAGGLF
                     VRNFHRSIEKEIKRSSRTRAPFSILLGHVQFGEDIPLSKAEQEDAPIGEVEEDLNGVF
                     DAYLSEIGRILRETDMVAPFGLREFLTILPFTTREGAGMVRDKLYGVFIKAANSRRNH
                     LSLSLKLGCATYPEDGETRMALIAKAEEMAEI"
     gene            complement(1618..1881)
                     /locus_tag="PKH07_04675"
     CDS             complement(1618..1881)
                     /locus_tag="PKH07_04675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34274.1"
                     /translation="MLVQFFEMSSVGGSEDLYRFKSHVNHWLEKESDKIHIHFVKQNV
                     VEKQGAIVSIISVWYEPKEKGVLPADDEPESTQKAQRVSFPSL"
     gene            complement(2226..3035)
                     /locus_tag="PKH07_04680"
     CDS             complement(2226..3035)
                     /locus_tag="PKH07_04680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNT34275.1"
                     /translation="MRHQVSFSGLARLEMVQKSMRTGVFLLAFSSFYTFFLGCASERL
                     HTMDSRIGHAERQMKEMIDKNEQKSQEHDRRVEIMQKQLEEENRLLQQQQQETLQSLD
                     SLRMALSGEKPVVEPQDPPAQEPAQTAAVLSPNSDGSAAVMGTQGDARFQAAYADYAR
                     GSYDLAIEAFKMLRDSYSEGRDRARIEYYIAECYYGKQEYAQALTYFAAVGNFASDSA
                     LLAPALWKRAMCFHSLRLPARRTEILRDLIQRFPDSDEAGMAQEWLTGETP"
     gene            complement(3051..>3834)
                     /gene="dapA"
                     /locus_tag="PKH07_04685"
     CDS             complement(3051..>3834)
                     /gene="dapA"
                     /locus_tag="PKH07_04685"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HNT34276.1"
                     /translation="HQEHKDVVSFAVKHLESLSKQGKRVPFLIAGAGSNSTREAVELT
                     AHAKKAGATVCLSITPYYNRPTQAGLFAHYTAIADEVGLPIIPYNVPSRTGVNLEADT
                     IVRLSEHPLIVGVKEASGNTTQISEIVRRTRDDFLVFSGDDAMTLSVLAMGGDGVISV
                     SANIAPGDMTEMCRLAMAGDFIKARRLHHKQVPLIQALFRETNPIPVKTAMNLLAGQA
                     SFSGAPCWPDVGELRSPLVPISDANLRHLKTAMREYGLSVPE"
BASE COUNT          953 a         1128 c          906 g          847 t
ORIGIN      
        1 tctgaacccg gaccgtgctg agcatacata tgaggcggat tggtcgaagg ggggggcgta
       61 tcagcagacc aggcaggaac cgggtttatg taggcatgca gttccaagcc gcgtttgtgg
      121 gcctcctgaa tggcaaactc caagggatcc cagcctggat cagcacttcc aaaaaccggt
      181 gaccatggct cgatgggact ggggtagagt gtatcggcct gtccacggca ctgaaacatc
      241 actgcgttga aattggcctt ctgacactca tctaaaatgg aaagaatctt gcctttggaa
      301 acttcaggat tcctgtcagg ccactcaaat cgagtgaccc acaatgctct gaactcctcc
      361 tgcgcagcgg aatccgaagc agcccaacat gacaacacaa caacgactgc tacgagaact
      421 gagaaaacaa agtacctcac aattcaactt cctctctttc ttccaagact tctcacaaaa
      481 caatacactt cctcacaaaa cccgtcttct acaaaaaaca gagcctcctc agaacaaaaa
      541 ctggcaagtc actcgggagc aaggaagatc gtcgttctgc agtggccact tgaaagatgc
      601 gccgtctttg gatcacaccc gctggccatt actgaagagc attgcccttt caaatctccg
      661 ccatctcctc ggccttggcg atcaacgcca ttcgcgtttc cccatcttca ggatacgtcg
      721 cgcaaccaag tttgagggac aggctcagat gattcctccg cgaattagcc gccttgatga
      781 acactccata caacttatct ctgaccatcc cagcaccctc acgggttgta aagggcagaa
      841 tcgtcaagaa ctcccgcaag ccgaatggcg caaccatatc cgtttcacga agtatcctgc
      901 caatctcgga caggtaggcg tcaaagacac cgttgagatc ctcttccacc tcaccgattg
      961 gagcatcctc ctgttctgct ttactcaggg gaatgtcctc accaaactga acgtgtccca
     1021 gaagaatgct gaagggtgct ctcgtccgac ttgagcgctt gatttccttc tcgatggagc
     1081 ggtggaaatt tcggacaaag agcccgccag catacatggg tccaaaacgt tccagaccaa
     1141 gcaaccggtt cagttgcatt accaaccggg gctttgatat cggcagaaga agatagtctg
     1201 tcgccccaca ggcaatgctg ctcatcagca gcgagtagtg cctgtcttca ctcaccatga
     1261 ccaaagccgc ggtgcccaga gcgggatggc actgaatccg acggatctcc tcaaccgcac
     1321 gagcctccgt tctgggttgt tccagaaaga tcacatccgg gggttcggca aaggcctgcg
     1381 gaagcgtgaa agactccgga gccaactccc gcacactact tgcggagcga accagcagac
     1441 catcctgaca caactcagag gcaagcgcag aggctacaga gggcgttggg gcaacgaaat
     1501 aaacgcgaaa gagtcgctgt ttgacctgag tcggcatcac gtctgagatc aatgaaatct
     1561 tgtcctggtc taccatggac tcctatccgt catcatcgct ccgttgcagc atacagctca
     1621 gagcgaaggg aaacttacac gctgcgcctt ctgagtgctc tcaggctcat catcagctgg
     1681 tagaacaccc ttttcctttg gctcatacca caccgagatg atcgacacaa tagcaccctg
     1741 tttttcgaca acattctgct taacgaaatg aatgtgaatc ttatcggact ccttttccag
     1801 ccaatgattc acatggctct tgaagcgata gagatcttcg cttcctccaa cagatgacat
     1861 ctcgaagaac tggaccaaca tagccgacct cctgctctgc actcctaaga ctgcccttgg
     1921 tttttggtct catgcctgtt ccggttcctc cacccacctc tcttgaaaga caggcgagca
     1981 cgaagccccc cgtgtgggag acctccggat ttggaacccc ttaccccaac cctctcccgc
     2041 agaaagagag agggagccgg agtctctgac ttgggcgaac acgcgcgagt gtgaacacag
     2101 tccacactcg ctcatattga cccgctccca ggaaactgcc cgataaacgg gacagatttc
     2161 gcttcaaata gacgttcctg caggtcggca aagcccaagg gctcgcctgt gccggcaggc
     2221 tcatcctatg gggtctcccc cgtgagccat tcttgtgcca tgccagcctc atcagaatcc
     2281 gggaaacgct ggatcaagtc tcggagtatt tccgtgcgtc tcgcgggaag acgaaggctg
     2341 tgaaaacaca tcgccctttt ccacagagct ggagccaaaa gtgcgctgtc tgaggcaaaa
     2401 ttgcctacag ccgcaaaata agtaagagcc tgagcgtact cttgttttcc ataatagcac
     2461 tcggcaatat agtactcaat acgtgcgcga tctcgtccct ccgagtagga atcccgaagc
     2521 atcttgaacg cttcgattgc gagatcgtat gagccacgag catagtccgc ataggcggcc
     2581 tgaaatcgcg catccccctg agtccccatc accgcagcag acccgtccga gttcggggac
     2641 aaaactgctg ccgtctgagc aggctcctgt gcaggtgggt cctgaggttc aactaccggc
     2701 ttctcaccag acaaagccat ccttagactg tccaaactct gaagagtttc ttgctgttgc
     2761 tgctgcaaaa gccgattctc ctcttccaac tgtttctgca ttatctcaac tcggcggtca
     2821 tgttcctgcg acttctgctc attcttgtcg atcatctctt tcatctggcg ctctgcatga
     2881 ccgatccgtg aatccatcgt atgcagcctt tccgaagcac aacccagaaa gaaggtataa
     2941 aacgaagaaa acgcaagaag gaaaacgcct gttctcatcg atttctgaac catctcaagt
     3001 cgcgcaagac cagagaacga cacctgatgt ctcattcgta cactccacac tcactcagga
     3061 acactcaaac cgtattccct catagcagtc ttaaggtgcc tcaagttggc atcggagatt
     3121 ggcaccaaag gcgagcgcag ttcgccaaca tccggccaac agggcgctcc ggaaaatgac
     3181 gcctgccccg cgagcagatt catggccgtc ttcacaggaa tcggattcgt ctcccgaaac
     3241 agagcctgaa tcagaggaac ctgcttgtga tgcagtctcc ttgctttgat gaagtcacca
     3301 gccatggcca gccgacacat ctctgtcata tcgccaggtg caatgtttgc cgagaccgag
     3361 atgacgccat cgccacccat cgccaacacc gaaagagtca tggcatcgtc accagaaaac
     3421 acaagaaaat cgtctcgcgt ccttcggacg atctcgctaa tctgggtcgt gttgccactg
     3481 gcctccttaa cacccacaat cagagggtgc tctgaaagtc tgacgatcgt gtcggcttcc
     3541 agattcactc cagtccttga cggaacattg tagggtatga tgggtagacc cacttcatcg
     3601 gcgattgccg tgtagtgcgc gaacaaccct gcttgcgtcg gacgattgta gtatggggta
     3661 atggataggc agactgttgc tccagccttc tttgcatgcg ctgtcagttc gactgcttca
     3721 cgcgtactat tgctgcctgc ccccgcgatg agaaaaggaa ctctcttgcc ttgctttgaa
     3781 agactctcaa gatgtttgac agcgaatgag accacatcct tgtgctcttg atgg
//
LOCUS       DAOQUC010000197         3817 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_27051_length_3817_cov_2.641946, whole genome shotgun sequence.
ACCESSION   DAOQUC010000197 DAOQUC010000000
VERSION     DAOQUC010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3817
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27051_length_3817_cov_2.641946"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(152..>1163)
                     /locus_tag="PKH07_04690"
     CDS             complement(152..>1163)
                     /locus_tag="PKH07_04690"
                     /inference="COORDINATES: protein motif:HMM:NF014788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HNT34277.1"
                     /translation="AGRIGEISSAEDSFGGLRFARFSGTDILVQMNFDGIGTKVEVAE
                     RLNRHNTMAFDLFAMVCDDAAIRGLEPIHIGSVLDFRTVNADVVQALADGMVAAARAA
                     GVAVINGELAELGERVGGYGPSAYNWAATVLAVGKRERLLDTGRVRAGQYLVAVREQG
                     FRSNGFTLVRKAVMGYFGPRWHEQPWEGKTVGEYVLTPSTIYTPLLVSLFGGFDAEPK
                     GSLIAAAHITGGGIPGKLGRCLKHSGCGALLDDLFEPVRMMLDLQKVGGISDRDAYQA
                     WNMGQGLIIVTDDPDSVLDEASRLGFEAKQAGTVIATPEIQIVNKGAYKESEPRLAFP
                     CS"
     assembly_gap    1164..1173
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1379..2377)
                     /gene="holB"
                     /locus_tag="PKH07_04695"
     CDS             complement(1379..2377)
                     /gene="holB"
                     /locus_tag="PKH07_04695"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HNT34278.1"
                     /translation="MTQQNNLLGQNHIWMPFFAAIKQGRIAHAYLVQGPSGSTYMDVL
                     RAMAKALLCQEVLGGFCDLCKVCLRVEHGTYADFHVIVPEAGKITLDKVRGVLDMAYQ
                     YPMEGSRRVFLIQDPERMNIESANMLLKVLEEPPAGTFFLLGTENSAGVLPTIASRCQ
                     AFRLRPLPEQEIQSHLERELGLEPAQAMAIARLADGSLGSARALADADFVERRQQAIG
                     LLGQLLTRADQAFAMEACKSLASECARDRESLIAFVRICLGLLRDAAVLNSGGSFENL
                     IHGDVGTEIQSMARLRPSRFWPLKIELTLDLVRKIRANLNTELVLGNYFLALQDEE"
     gene            complement(2534..3550)
                     /locus_tag="PKH07_04700"
     CDS             complement(2534..3550)
                     /locus_tag="PKH07_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017938607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HNT34279.1"
                     /translation="MPLVPMRQILDEAKKKGYGVGAYNVNNMEQIQAIMQAARETKSP
                     VILQASKGALEYTSMVYIKHLMLAAMEENPDIPLAMHLDHGPNFDLCKKSIELGFTSV
                     MIDGSIDYAVKDDKGKHPPRSFEDNIKVTKEVVDYAHKFGVSVEGELGTLGGIEDETV
                     ASKVQLTDPDQCQEFIEKTGVDALAIAIGTSHGAYKFKTEPKLAMDLISDIHKRVPYA
                     ALVMHGSSSVPAELVDRVNRFGGQLAKTMGVPVESIQEAVKRGVTKINVDTDGRLACT
                     GAVRQYLAENPKEFDQRKYLGATRVAIYEVIKSRMISFGTAGHAGDYAPMSLDDMKKI
                     YASK"
BASE COUNT          916 a         1075 c          946 g          870 t
ORIGIN      
        1 gcggttagag cgttcgcctg ataagcgaaa ggtagaaagt tcaactcttt ccaggcccac
       61 cactcagtga tagcgagaag aatgccccaa caagatgggg cattttgttt atggacttct
      121 gaaaactgcc gtggggtata aaaccaaagg gctaagagca cggaaaagca agccttggct
      181 cgctctcttt gtaagcgccc ttattcacga tttggatctc cggtgttgcg atgaccgtgc
      241 ctgcctgctt tgcttcgaat ccaagacgtg aggcctcatc cagaaccgaa tcgggatcat
      301 ccgtcacgat gatgagtccc tgtcccatgt tccatgcttg atacgcatca cggtctgaga
      361 tgccgccaac tttctgaaga tcaagcatca tccgcaccgg ctcaaaaagg tcgtccagta
      421 gagcaccgca tccggagtgt ttgagacagc ggccaagttt ccctggaatc ccaccacctg
      481 tgatatgagc tgcggcgatc aaagagccct tcggctcggc atcaaatccc ccaaacagag
      541 aaacaaggag tggcgtgtaa attgtggatg gcgtaaggac atactctcca acggtctttc
      601 cctcccaagg ttgttcgtgc catcggggac cgaagtagcc cataaccgcc ttgcggacca
      661 aggtaaaacc gtttgatcga aatccttgtt cccgaaccgc cacaagatac tgacccgctc
      721 gaactctgcc ggtgtcaagc agtcgttcac gctttccaac ggcaagcacc gtcgccgccc
      781 agttgtaggc actgggccca tatccgccaa cacgctcgcc gagctccgcc aattcgccat
      841 tgatcacggc cacacccgca gcacgtgcgg cggccaccat accatccgcc aacgcctgca
      901 ccacatcagc gttgaccgtt cggaaatcga gcacggaacc gatatggatc ggctccaaac
      961 cacgaattgc cgcgtcgtcg cagaccatgg cgaacaagtc gaaagccatg gtgttatgcc
     1021 gattcaggcg ttcagcaact tccacctttg tcccgattcc atcaaagttc atctggacga
     1081 ggatgtccgt gccggaaaac cgggcaaaac gaagtccccc aaacgagtct tcagctgagg
     1141 atatctcgcc aatcctgcca gccnnnnnnn nnnggcctca tagagaatcg ccgagcactg
     1201 atctcccagt ttcacatcca cacccgcttt ggcgtatgcg ctggaatctg tcttttccat
     1261 catgattcgt ttgctccaag aataagtctt tctgttcgca gatatcttgc ttgttcccaa
     1321 aattcgagcc caaggcagtt tgtaacctct cattctctgc aagcctgcaa agaggttctc
     1381 actcctcatc ctgcaaggca agaaaatagt tgcccagaac cagttccgta ttcagattag
     1441 cacgaatttt tcgcacgaga tccaaggtta gctcaatttt cagtggccag aaccgagacg
     1501 ggcgaagtct cgccatggac tgaatctcag tgccgacatc tccatgaatc aggttctcga
     1561 atgagcctcc agaattgagt acggcggcat ctctcagaag tcccaggcag atacgaacaa
     1621 aggcaatcag cgactcgcga tcccgtgcgc attcggaggc aagactcttg cacgcctcca
     1681 tggcaaaggc ctgatcggct cttgtgagga gttgcccaag cagaccgatt gcctgctgtc
     1741 tgcgctcgac aaaatcggca tcagcaagcg cccgagcact cccgagactg ccgtctgcaa
     1801 gtcgggcaat tgccatcgcc tgagccggtt ccaaacccag ttccctttcc aagtgactct
     1861 ggatttcctg ctccggcagc ggtctgagac gaaaagcctg acatcgcgag gcgatagtcg
     1921 gaaggactcc agcgctgttc tcggttccaa gcaagaagaa ggtgccggcg ggcggttcct
     1981 ccagaacctt cagcagcata ttggcagact caatgttcat tcgctctgga tcctggatca
     2041 gaaacactcg acgggagcct tccatcgggt attggtatgc catgtccaaa acaccccgaa
     2101 ccttgtccaa cgttattttg cctgcctcgg ggacgatgac atgaaaatcg gcataggtac
     2161 catgctcgac acgaagacag accttacaga ggtcacagaa accacccaga acttcctggc
     2221 atagcagcgc ctttgccatg gctcgaagca cgtccatgta ggttgaacca ctgggtccct
     2281 ggaccagata agcatgtgca attcgacctt gtttgatcgc agcaaagaag ggcatccaga
     2341 tatggttttg gccgagaaga ttgttctgct gtgtcatttt cctgctgttc cggatgttca
     2401 gtccaagaat atattatgcc agcacggttc gaagaagtct actgcaagcg cattcggaaa
     2461 gttgcggtaa atgaaatccc ttcccgacat gagtcaggaa gggattcact ttgatatctg
     2521 tctgagcggt cggttacttg gaagcgtaaa tctttttcat gtcgtccaaa gacatgggag
     2581 cgtagtcgcc tgcgtgacca gcagtaccga aggagatcat tctggacttg atcacttcgt
     2641 agattgcgac acgcgtggca cccaggtact ttctctgatc gaattccttg gggttctctg
     2701 ccaagtactg acgaacagca cccgtgcagg cgagacggcc atcggtgtca acattgatct
     2761 tggtaacacc acgcttgacg gcttcctgaa tggattcgac tggaacgccc atcgtcttgg
     2821 caagctgtcc gccaaaacga ttgacgcgat ccaccagttc cgccggaacg ctggacgaac
     2881 cgtgcatgac gagagcggca tacggaacgc gcttatggat atcagagatg agatccatgg
     2941 caagtttcgg ctcggttttg aacttgtacg cgccatggga tgttccgatg gcgatagcca
     3001 gagcgtccac accagtcttc tcgatgaatt cctgacactg atccggatcg gtcaactgga
     3061 ctttggatgc gacggtttcg tcttcaatgc cgccgagggt tcccagttcg ccttcaacgc
     3121 tgacgccaaa cttgtgagcg tagtccacca cttccttggt gaccttaatg ttgtcctcga
     3181 aactgcgggg cgggtgctta cccttgtcat ccttgaccgc gtagtcaatg gagccatcga
     3241 tcatgacaga ggtaaatccg agctcaatgg atttcttgca caggtcgaag ttgggaccgt
     3301 gatccaaatg catcgccaga ggaatgtcag ggttctcctc catcgccgcg agcatcagat
     3361 gcttgatgta gaccatgctg gtgtattcca gcgcaccctt gcttgcctgg agaatgaccg
     3421 gactcttggt ctcccgcgca gcctgcatga tcgcttggat ctgctccatg ttgttgacgt
     3481 tgtacgcgcc gacaccgtag cccttcttct tggcttcatc cagaatttga cgcatgggta
     3541 ctaaaggcat gatatctaaa ctcctccttc tattatttca gggcaatgcc ctgattcttc
     3601 tcaggttaag agcctgacag gctcctgcag tcggctccgg cgtacaaaaa aaaagaaaac
     3661 atccacccaa ggactatacg agcctacaaa attagaacgg ttatcgaagc gtaaaggaac
     3721 gctccgcact ccaaggtccg aaaagcagag tacttggatc ttgtcctcgc accttccaac
     3781 gatagccacc ggttatcgtt gacatgaaag agaatgt
//
LOCUS       DAOQUC010000198         3816 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_27062_length_3816_cov_3.302047, whole genome shotgun sequence.
ACCESSION   DAOQUC010000198 DAOQUC010000000
VERSION     DAOQUC010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3816
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27062_length_3816_cov_3.302047"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(272..1396)
                     /locus_tag="PKH07_04705"
     CDS             complement(272..1396)
                     /locus_tag="PKH07_04705"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HNT34280.1"
                     /translation="MCWEIGDLCIELLDEHRLSLGQIAQFTDYSRTRVSHFHLTARTF
                     GRGQRRGYTFQDSLIARQIHKKLPRLNMTPLEIREVIVRMKNKTPAKVHAHFVRLLTE
                     KERNQHLALAGRPLTEGGGLINQCHRADWRDIIPQLPDQSVQLFLCDPPFAISKGYLS
                     NLAATNALRIDCDYGLTEQEALEVTLPLFELCLSKLCSDGVLLLFQGGGKPDRLEVLQ
                     KAQDWGWACHYALTWNKGAMSAGNFQKPYLIWSERILVFGRSGEKLKKHQNGSPSSDI
                     LRFQPETRHATQRMHAGKLAYRDCHMFQKPPKLMEFLTQHHSLPGDLVVEPFGCSGSG
                     VIAAAKLNRKWVYVESNHENYLWGSQRVRKALSEISVQAG"
     gene            2493..3779
                     /locus_tag="PKH07_04710"
     CDS             2493..3779
                     /locus_tag="PKH07_04710"
                     /inference="COORDINATES: protein motif:HMM:NF014273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HNT34281.1"
                     /translation="MKQKQMWVVGSVEFTALFEDLIPRLSVEEYERLKEDVAAHGVLV
                     PVAVDEKMQVIDGINRIKAASELEHRSIPIHVHADLAANEKKELAIRLNAFRRHWSQD
                     ERLAMTVALRREKYSLRRIGKILNLSHETVRRYLEASGVAKDLPDTVVGDDGIERPAK
                     VTRRPVIALKNTKEAKEAYRQLEEAEGIHLPNSIIDVKRLGRLVRDHKSKTYNRADLS
                     DRTVGQATLLLGDFAEKGETVAPDSVDLIFTDPPYGGDYLGLWDELGRFAEKILKPGG
                     LLLSYSGVLYLPQIYEMLGRHLKYFWTFAVRHTGGRKTVYSVNVHQAWKPIVGFFKPP
                     FKKQWHTFPDMTTAGQSKEFHKWEQPIGEAHYFIKNLCPAKGVLVDPMMGSGNVILAG
                     MALGMTCTGIEIDTTAFATAQQRIEQVQKKLGKEAA"
BASE COUNT          923 a          970 c         1040 g          883 t
ORIGIN      
        1 ggtttctggc agaaatcgac tacctcaaag tggctcgtgt ttctatgtat agtattattg
       61 taggccgttt ttttctaccg tggttttttg ggaaagacac caaacagggg catactgggt
      121 ctgacagaat ggaaaacacg atatgctggt aggtggggca ctgtgctccc tcactctaca
      181 cagtatatgt gaccacgata gagactgact gccgtggaga aaggccggtt cgctaattca
      241 aactgtcaca tcctgtggca gtcctggagg cctaccccgc ctgcaccgag atttcagaca
      301 gggccttacg aacccgctga ctgccccaca ggtagttctc atggtttgat tccacgtaga
      361 cccacttgcg gttgagtttt gccgccgcaa tcaccccact cccgctacat ccaaaaggtt
      421 caaccaccag atcccctgga aggctgtggt gctgggtgag gaattccatg agtttcgggg
      481 gcttctggaa catgtgacag tcccggtagg ccagcttccc agcatgcatt ctttgcgtgg
      541 catggcgggt ctcaggctga aatctcaata tgtctgaact gggagagcca ttttggtgtt
      601 tcttcaattt ctccccgctt cggccgaaaa ccagaatccg ctccgaccag atgagatagg
      661 gcttttggaa gttgcccgca gacattgccc ctttgttcca ggtcagggca taatgacatg
      721 cccatcccca gtcttgggcc ttctgaagca cctccaggcg gtcgggtttt cctcccccct
      781 gaaacagcaa caagacgcca tctgaacaca attttgacag gcacagttcg aagaggggga
      841 gcgtcacctc caaggcctct tgctccgtta gtccgtagtc acagtcgatt cggagggcat
      901 tggtggccgc caggttcgag agataccctt tcgaaatggc aaatggagga tcgcacagga
      961 agagttggac ggactggtcg gggagttgcg ggatgatatc ccgccagtcg gcacggtggc
     1021 actggttgat caggccgccc ccctctgtca ggggtctgcc ggccagggcc aagtgctggt
     1081 tcctttcctt ctccgtcagg agtcggacaa aatgggcatg gaccttcgcc ggggtcttgt
     1141 tcttcatccg cacgatgacc tctcggatct ccaagggggt catgttgagg cggggaagct
     1201 tcttgtggat ctgtcgggcg atgagactgt cctggaaggt gtaacctctt cgttgacctc
     1261 ttccaaaagt gcgggctgtc aggtgaaagt gactgacccg cgttcgggag tagtcggtga
     1321 attgggcgat ctgccccaga gagagtcggt gctcatcgag cagttcgatg cataagtccc
     1381 cgatctccca gcacatggaa tcctgctgat ggatgagctt tttgagggtc ttgagtttgg
     1441 ccaggacctc ggcaggcagt gttctgcatg tgtgctctac cctgattgag accgacctgt
     1501 gtgttcgtaa cccattgttc tgcaaagagt tagaaacctt cctcatgtcc gggctccttc
     1561 ccatggggcc gtgaacgcac atttacggct tcagggtacg cctatccccc ttccatgtcc
     1621 aggagttcct gttggcgggt tctttgaaac cgagcgtcga acaggagaag agggagccag
     1681 tgtgcctgtt gaaaaatttc tgtttccaga caaaattttt ttttccccat caaccaaaaa
     1741 accaaaaaag gacgaaacat gacgcctata cgacatcaaa agcacaccca taagcctccg
     1801 tgatggaggc accgctaaac ggcacgcctg gctgagaacg cgaagacaaa acgaaatgac
     1861 ggtttggggc aggctttctc tatgtccaga aagcagcagt gattgaggaa attctccatg
     1921 acaaaacaca atttcaatca gtattgggtt cgtcgattca caaggttttc tcctcatgga
     1981 aagagcatgt atatcaaccc tattcggaaa atctcggtgg cctacttttg ctttgaggct
     2041 ctccacgatt taggtgtcaa tcaatggcat ccctcaacgg ctgttatcac tcacttgaag
     2101 catctcatgg catcgtgcca ggcagcggat ggccaatcct attgggaaac ataccgcagc
     2161 aaggcgaaga gcaagaaggc ggatgtgtct caacgcctgc atcatatttt gacttccctg
     2221 tcatccctct acaaagaccg tcttactgag atgaattgca tcgttgaagt caagagaacc
     2281 gactctgaga tatatctccg cttgaacaca cattcgtcca cacctactca cgaatccatg
     2341 ggaattaaca agaacagcca aaagaaagcg ccttcttcta actgtcgatt attcaagttt
     2401 gccgccatgc ttaccgccct gttgagcata ttcacttcgc atgggcggta aaaaatcact
     2461 tattcaatca ccttttttga aaagggcatg tcatgaaaca aaaacaaatg tgggttgtag
     2521 gtagcgtgga attcaccgcg cttttcgagg atctgattcc cagactctcc gtggaagaat
     2581 acgaacggct gaaggaggat gtcgccgccc atggcgtctt ggttcccgtc gcggtcgatg
     2641 aaaagatgca ggtgattgac gggatcaacc gtatcaaggc ggcttcggag ctagaacacc
     2701 gaagcattcc catccatgtg catgccgatt tggcggcgaa cgagaaaaag gaacttgcca
     2761 tccgactgaa tgcctttcgc aggcattgga gtcaggatga gcggcttgcc atgaccgttg
     2821 ccctgcgcag agaaaaatac agcctgcgac gcatcgggaa gatcctgaat ctaagccatg
     2881 agactgtaag gagatacctg gaggcatcgg gagtggctaa ggatctgccc gacaccgttg
     2941 tgggcgacga tggaatcgaa cgacccgcga aggtaacacg gcgaccggtc atcgctttga
     3001 agaacaccaa ggaggcgaag gaggcttatc ggcaactcga agaagccgaa ggcattcacc
     3061 ttcccaactc catcatcgat gtcaagcggc ttggcagatt ggttagagac cacaaaagca
     3121 agacctacaa tagggctgat ctgagtgaca ggaccgtagg tcaggcgacc ttgttgttgg
     3181 gtgactttgc agagaagggt gagacggtgg ctcctgactc cgtagatctg atcttcacgg
     3241 acccgccgta cggtggggat tacctggggc tgtgggacga actcggccgc tttgccgaga
     3301 aaatactgaa accggggggg cttctgctga gctacagtgg ggttctgtat ctgccgcaga
     3361 tctatgaaat gctgggaagg catctgaaat acttctggac attcgcggtt agacatacgg
     3421 gcggccgtaa gacggtttat tcggtcaatg tgcaccaagc atggaagccc attgtgggtt
     3481 tcttcaagcc ccccttcaaa aagcaatggc acaccttccc ggatatgaca accgcaggac
     3541 aatccaaaga gttccacaaa tgggaacagc ccatcgggga agctcattac ttcatcaaga
     3601 acctgtgtcc cgcaaagggt gttctcgttg atccgatgat gggcagcggc aatgtgattc
     3661 tcgccgggat ggctttggga atgacgtgca caggaattga gattgacacg acggcgttcg
     3721 ctaccgccca gcagaggatt gagcaggttc agaagaagct cggtaaagag gcggcataga
     3781 cagtggaacg ggcagaagga tcggcttttc tgcccc
//
LOCUS       DAOQUC010000199         3814 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_27078_length_3814_cov_3.605746, whole genome shotgun sequence.
ACCESSION   DAOQUC010000199 DAOQUC010000000
VERSION     DAOQUC010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3814
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27078_length_3814_cov_3.605746"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3814)
                     /locus_tag="PKH07_04715"
     CDS             complement(<1..>3814)
                     /locus_tag="PKH07_04715"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HNT34282.1"
                     /translation="QAEPDWMYYKALVPNDRFYRQQYHLPRIRCEQAWNIGTGDPSVI
                     IGIVDSGTYTEHEDLKDRLWVNLGEIPNNGVDDDGNGYIDDINGWDFINDNNDVNPDP
                     DGEDDNDDDIPDDNVEHGTHVAGIAAAMTNNNIGVAGVSWQSQIMTAQVFPDDGATST
                     SYVLQGIIYVADNGANIVNLSLGGPYSSAFNRIFQDLHSRNIVIVCAAGNEYANLDIT
                     PVSPVCNDGKPDANWVIGVAATNSQDVKVGFSNYSAKFVDVAAPGLSILSTLPLFYQY
                     GFYDEYGSMSGTSMASPVVAGAAAVILGASPGISNSEMLGRISYFADNIDSLNPTFAG
                     QLGGGRLNLFSVLQTLDLPLPVIPTPTPVPHRYPEKDLVMPSSEPIAFIGMDLHYPSG
                     AARLQQITVRFRNRLESTGTLSLDDLAPLTLDELSGLTLYRDTGGPTRGGFDWNPLGS
                     GGSVDLLVPLRSISWRNLEGENGFEVILDIDQDPPLYKDASVIPAASDSGLFDFFVVV
                     KTSEKLAHDDAFKVDIPAGGIKALDKSVSGRPVALEYYDEDYPPEGETKVTYGEVVKF
                     VNFTQIESSEGEYQPIVQLSPPTAVLGINGWGHRIHNYSIAEVTVDLLNIKNFSPDML
                     NPLSRNQTTSGVALYRDMPGTTDGVFDGVEREQFVVLENIRWEMASEEIGAASWRIVL
                     TPETPQPLPEDDTSLGNVGADYFLVIRTSDRCRDGDSFRMEISQCDKSLPGANWRPMA
                     GRVTLVSGDQGDLRTRRILTNTSEAQYGERVHSIKARPRPFFAFHDTIVNTQIGVESR
                     TTPVLGIDMHDYGADCGVVADREHWGWILQEMTFDVISTNPNVPVRPEHFRPVLSNGA
                     RDGVFADYSTGIGIFMDDDTETGDGLDDDEIFFNPRATLRDEELRNNMDDDDDGLIDE
                     DCGDGDPPGIVNGAWDTDDDIIFFGARNYTADSTFANPPIVQTLAAEPGFRVTFNFIM
                     QRSCETHNCYAPGWSTADIVSEEPLGSNFYRLAEWPVVSETAVQCGDEGCDFSRVVYY
                     PTGMGFYPIGQPCPDLNPPCGTRVARYQHYIQIPNDNEGVLLGDDMFVTIRTHHLVPN
                     GLEFAFRVNSGAFSYTKYSSVGNENKYPGSSEGFLLTKPIRVVVDVFNELVDMTLQDQ
                     RIEQFSASDRVPVTAVGLNMDIWEHSESSPEAYEAEFSYEYIHEFLVELVDEGGFQLS
                     IDPTASDLLPLSGTAYSGIGLYFDDGITPGSLDPQDSMIELVRTTAEDVQKVGNSYRV
                     MLRPRSRD"
BASE COUNT          972 a         1020 c          928 g          894 t
ORIGIN      
        1 atgtcacgag atctgggacg cagcataact ctgtaactgt tgcccacctt ctgaacatcc
       61 tcagccgtcg tccgcaccag ttcgatcatg gaatcctgag gatccaagga gcccggcgta
      121 atcccgtcgt cgaaatacag cccgataccg ctgtaggcag tgccgctcag aggaagcagg
      181 tctgacgctg tcggatcaat ggacagttgg aaacctcctt catccaccag ttcaaccagg
      241 aactcatgga tatactcata gctgaactcc gcttcataag cctcaggcga actttcagag
      301 tgctcccaaa tgtccatgtt aagaccgacc gcagtaacgg gaacacgatc agatgcggaa
      361 aactgctcaa tcctctgatc ctgcagcgtc atatccacca gttcgttgaa gacgtccacg
      421 accactcgaa tcggctttgt cagcaggaat ccctcagaag aaccagggta cttattctca
      481 tttccaacac tggagtactt ggtgtaggag aaggcaccgc tattgacacg aaaagcaaac
      541 tccaagccgt ttggcaccag gtggtgggtt cgtattgtaa caaacatatc atcacccaac
      601 agaacccctt cgttgtcatt gggaatctgg atgtagtgtt gatagcgagc aacgcgtgtc
      661 ccacagggcg gattgagatc tgggcaaggt tgcccaatgg ggtagaaacc cataccggta
      721 ggatagtaga caaccctgct gaaatcgcag ccctcgtctc cacattgaac tgcagtttca
      781 ctgacaacgg gccattcggc aagacgatag aaattcgagc caagtggctc ttcgctgaca
      841 atgtcagcag tactccagcc gggtgcgtaa cagttgtgcg tctcacagga gcgctgcatg
      901 atgaagttga aagtcactct gaaacctggt tcagccgcca aggtttgaac gataggtggg
      961 ttggcaaagg ttgagtccgc ggtgtagttc cgtgcaccaa agaatatgat gtcatcgtcg
     1021 gtgtcccagg caccgttcac gatgcccggt ggatctccgt caccacagtc ctcgtctatc
     1081 aaaccgtcat catcatcatc catgttgttg cgcagctctt cgtcacgcag agtcgccctg
     1141 ggattgaaga atatctcatc gtcatcgagg ccatctcctg tctccgtgtc gtcatccatg
     1201 aaaattccga ttccggtgct gtaatcagca aaaactccgt ctctggcacc gttgctcagg
     1261 acgggacgga aatgctcagg tcttacggga acatttggat tagtactgat gacatcaaaa
     1321 gtcatctcct gaagaatcca cccccagtgc tctcgatccg ccaccactcc gcagtctgca
     1381 ccatagtcgt gcatatcaat tccaagaaca ggggttgtcc tcgactcaac accaatttgc
     1441 gtgttaacaa ttgtgtcgtg aaatgcaaag aatggcctcg ggcgcgcctt gatactatgg
     1501 actcgctcgc cgtattgtgc ttcggatgtg ttggtgagaa ttctacgtgt tcgaaggtca
     1561 ccctgatcgc cactgaccag agtcacacga cccgccatag gacgccaatt ggctcctgga
     1621 agtgatttgt cgcactgaga tatttccatt cggaaagaat caccgtcacg acatctgtcg
     1681 ctggttcgaa tgacaagaaa ataatcggct ccaacattcc ccaaagaagt atcgtcttcg
     1741 ggaagcggct gaggcgtctc gggcgtcaga acaattcgcc atgaagctgc cccgatctcc
     1801 tccgaggcca tttcccagcg gatattctcg agaacaacaa actgctcgcg ttcaacaccg
     1861 tcaaatacgc catccgtcgt gccaggcata tctcgataga gcgccacgcc ggaagtagtt
     1921 tgattgcgtg acaatggatt gagcatatcc ggactgaagt tcttgatatt tagtagatcc
     1981 acggtcactt cggcgatgga gtaattgtgg attctgtgtc cccatccgtt tataccgagg
     2041 acggctgtcg gtggtgacag ttggacaatg ggctgatatt ctccttcgga cgactcaatc
     2101 tgggtgaaat tgacaaactt gacgacttct ccataagtga ccttggtctc tccttcggga
     2161 ggatagtcct cgtcatagta ctccaaagca accggtcttc cgctcacgct cttgtccagc
     2221 gccttgattc cgccagcggg aatatctacc ttgaaggcat cgtcgtgtgc cagtttttcg
     2281 ctggttttga caaccacaaa gaagtcaaag agcccgctgt cggacgcggc tggaatgacg
     2341 gaagcatctt tgtacaaagg gggatcctga tcaatatcaa gaataacttc gaaaccattc
     2401 tctccctcaa gattgcgcca ggagatgctt ctaagaggaa cgagcaaatc tacgcttcca
     2461 ccagaaccca aaggattcca gtcaaaacca ccccgtgtcg ggcctccggt atctcggtaa
     2521 agagtcagtc cagaaagctc atcaagggtg agaggtgcaa gatcgtcaag tgatagagtt
     2581 cctgtggact ccaaacgatt tcgaaatcga accgtaatct gctgaagccg cgccgcacca
     2641 gatggatagt gaagatccat gccgataaag gcaattggct cactggatgg catcacaaga
     2701 tccttctctg ggtatctgtg aggtacagga gtgggggtcg gaatcactgg aaggggtagg
     2761 tcgagagtct ggaggacact aaaaagattc agacgacctc cccccagttg tcccgcaaac
     2821 gtcggattga gcgagtcaat attatcagcg aagtagctga tacgacccaa catctctgaa
     2881 tttgaaatcc ctgggcttgc acccagaatg accgcagccg cacccgcgac aaccggactt
     2941 gccatcgagg tgcccgacat gctgccgtat tcgtcataga aaccatactg gtaaaaaaga
     3001 ggaagcgtgc tcaggataga cagacccgga gcagccacat ccacaaactt tgcactataa
     3061 ttcgaaaagc caaccttgac gtcctgtgaa ttggtggccg caacaccaat aacccagttc
     3121 gcatcgggct tgccatcgtt gcagacggga cttaccggtg tgatgtccag gttcgcgtat
     3181 tcatttcccg ctgcacagac aattacgata ttgcgtgaat gcagatcctg aaagattctg
     3241 ttgaatgcag aactgtatgg tccgccgaga ctcagattaa cgatattggc tccattatca
     3301 gcaacgtaga tgataccctg aagaacatac gaagtagaag tagctccatc gtcgggaaag
     3361 acctgcgcag tcatgatttg gctttgccag gaaacccctg caacaccaat gttgttgttc
     3421 gtcatcgctg cagcaattcc agcgacatgt gttccatgct cgacattgtc atccggtatg
     3481 tcatcgtcat tatcatcttc accgtcagga tcaggattga catcattgtt atcattgatg
     3541 aagtcccagc cattgatgtc gtcaatataa ccgttcccgt cgtcgtcaac accattgttc
     3601 ggaatctcac caagattgac ccacaggcga tccttgagat cttcgtgttc ggtgtaggtt
     3661 cctgaatcca caattccgat gataacactc ggatcgcccg tcccgatatt ccacgcctgc
     3721 tcgcaacgaa tccgcggaag atgatactgc tgacggtaga accgatcatt tggcacgagc
     3781 gccttgtagt acatccagtc aggttcagcc tgct
//
LOCUS       DAOQUC010000200         3813 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_27087_length_3813_cov_3.396487, whole genome shotgun sequence.
ACCESSION   DAOQUC010000200 DAOQUC010000000
VERSION     DAOQUC010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3813
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27087_length_3813_cov_3.396487"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..448
                     /locus_tag="PKH07_04720"
     CDS             <1..448
                     /locus_tag="PKH07_04720"
                     /inference="COORDINATES: protein motif:HMM:NF027440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP citrate lyase citrate-binding
                     domain-containing protein"
                     /protein_id="HNT34283.1"
                     /translation="ASGGGTSVEALDNLCLLGGKPAVFTEYSGNPSAEKVKGLTRIAL
                     MTEQPIDGLWVIGGRANFTDIYETIVSGFLEGVRETPNFDRTIPIVVRRAGPRDEEAF
                     RTLRKAREEEGYNILVRGMGTSVSESARMMINQARKHEQSRLQGKS"
     gene            445..2610
                     /locus_tag="PKH07_04725"
     CDS             445..2610
                     /locus_tag="PKH07_04725"
                     /inference="COORDINATES: protein motif:HMM:NF012506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HNT34284.1"
                     /translation="MRPFVGKDTRVLIQGITGRQGTKVCREMLDYGTHVVAGVTPGKI
                     GHSVHGVPVYSSVEEALKKHPEINTSLITVPRDAALAAAFEVIPFAQIRLVNVLTEGI
                     PQQDSARMVQLAREHRVWLVGPASVGIIAPGEHLKIGAIGGNDPGVFYPGPIALFSKS
                     GGMCLSIATELFNRLGYGNSLVVGIGGDRITGTTFVDLLELVRDDETTQLVIIMGEVG
                     GTYEENAADYIRRTKYPKPVVVRLTGIGAQNLFPKGTRMGHAGAIIGEERVGTFQSKV
                     EAFESIGVPVAKTSDELADLVEKAMPQAHVSDLESAVAGEMELVSISKTKLESLKSQV
                     RAVQVRTSMTQLIGGIPYFRGYLLTDLVQRATIPDMVWMTTKRRDTSRESSCQIQQDL
                     LWLAEHCPLTEKAIQSSWNCQQDGGRIETAISTGLLAQKEPDLQQFEGKEIRTRYSDD
                     ELLSLIIGSHVIALVSHLMEHPTDQVRATSLAELIGFALKGEPVDDKASRVLQAIFVA
                     CVDHTPATPSSLAALAAYSGGSTLKNALASGISAMGHVHAGAGEATAQILQDYLKRFR
                     TQKPMVADGIKIETIQGLAEYIVDKYTGVLGGEKRKLPGFGHRYYSLYRKDPRAESVM
                     QITREMALDADYVNLAHHLEESLKMRKSPGLCLNVDGVIGVVLSALGLPPKTGKALFI
                     IPRTFGIVSQLLEQEAGSFFRLANESVLYVGPDTGRTFE"
     gene            2664..2855
                     /locus_tag="PKH07_04730"
     CDS             2664..2855
                     /locus_tag="PKH07_04730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34285.1"
                     /translation="MPAQPFEGFFPEELSKAIQTYFEKFGLECHLREWVVSKIPGMEE
                     FDVTMKIHANRKASETRIT"
     gene            2966..>3813
                     /locus_tag="PKH07_04735"
     CDS             2966..>3813
                     /locus_tag="PKH07_04735"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNT34286.1"
                     /translation="MRNLSKRYNKLLALSELNLTVAPGEVFGLIGPNGAGKTTLLRIL
                     GTLCEPTGGEAHVFGINVQEQPRAVHAMIGFMPDFYALYDDLKVWEYLDYFAGLHGIA
                     RDQRRKKCDEMLARVNLEVKRDALVGETSRGMKQRLCLARALIHEPQLLLLDEPASGL
                     DPQARLELRDLIRQLQSEGKTLIVSSHILTELSGFCTSFGLLEKGVLVKSGRLEDIQK
                     EQPSRIVFLDVLEDSARVASVLAQFPSLSVTTHEGQQYVLSLTGQREDIAQLHRHLVQ
                     AEIPVVS"
BASE COUNT          929 a          970 c         1089 g          825 t
ORIGIN      
        1 cgcctcaggg ggaggcacaa gtgtggaagc acttgacaac ctgtgcctgc tgggaggcaa
       61 accggcagtg ttcacggaat acagcgggaa cccgtcggca gagaaggtca aaggcttgac
      121 ccgcatcgcg ttgatgacag aacagcccat tgacggcctc tgggtcattg gcggcagagc
      181 gaactttacc gacatctacg agacgattgt aagtggcttt ctggaagggg ttcgggaaac
      241 tccgaatttc gacagaacga ttcccatagt cgtccgacgg gcaggccccc gtgatgaaga
      301 agcgttccgc acgcttcgaa aagcccgtga agaggaaggc tacaacatcc tggttcgggg
      361 tatgggcacc tcagtcagcg aatcggcacg gatgatgatc aatcaggcga ggaagcacga
      421 acagtcccgc cttcaaggaa aatcatgaga ccttttgttg gaaaagatac ccgagttctc
      481 attcagggaa tcacaggcag gcaagggacc aaagtctgcc gcgaaatgtt ggattatggc
      541 acacacgttg tcgcgggcgt gactccgggc aagatcggtc acagcgtcca tggagttccc
      601 gtctacagct ctgttgaaga ggctctcaag aagcatcccg agatcaacac gagcctgatt
      661 acagttccgc gcgatgcagc tttggcagcc gctttcgaag tcatcccctt tgcgcagatc
      721 cgccttgtca acgtgctgac cgaaggaatt ccgcaacagg actccgcgcg tatggtgcag
      781 ttggcacgag aacaccgtgt ctggcttgtg gggcctgctt cggtgggaat tatcgctccg
      841 ggagaacacc tgaagattgg tgccatcggt ggcaatgatc ctggagtgtt ctatcccggc
      901 cccatcgcgc ttttttccaa gagcggcggg atgtgccttt ccatcgccac cgagttgttc
      961 aaccggctcg gctatggaaa cagtcttgtg gtgggcatcg gcggcgacag aataacgggc
     1021 accacattcg tggatctgct cgaactggtt cgagatgatg agacaactca actggtcatc
     1081 attatgggtg aagtgggggg aacctacgag gagaacgccg cagattacat ccgaaggaca
     1141 aagtatccaa agcccgttgt cgttcgtctg accggtattg gcgctcagaa cctcttcccg
     1201 aagggaaccc gaatgggtca cgcgggagca atcattggtg aggaacgtgt cggcacattt
     1261 cagagcaaag tcgaggcttt tgagagcatc ggagttcctg tggccaagac ttccgacgaa
     1321 ctggctgact tggtcgagaa ggcaatgccc caggctcatg tgtcagatct ggagtctgcc
     1381 gttgcgggcg agatggaact ggtcagcatc tccaagacga aactggagag tctcaagagc
     1441 caggttcgcg cggttcaggt cagaaccagc atgacccagc tcatcggtgg aatcccttac
     1501 ttccgaggct atctactcac ggatcttgtc cagcgcgcaa caattcctga tatggtctgg
     1561 atgaccacca aacgcaggga tacaagtcga gagtcgtcat gtcagattca gcaagactta
     1621 ctctggcttg ccgagcattg tcctctcaca gagaaagcca ttcagtcatc atggaactgt
     1681 caacaagatg ggggacgtat tgaaacggcc atcagcaccg gtctgttggc gcagaaggaa
     1741 ccggatctgc agcagtttga gggcaaagag attcgcacac ggtatagcga tgatgagttg
     1801 ctgtctctga taattgggtc gcatgtgatc gctctcgttt cgcatctgat ggagcatccg
     1861 accgaccaag tgagggcgac atctttggcg gagttgatcg gctttgcgct caaaggagaa
     1921 cctgtggacg acaaggcatc cagggtactt caagcgatct tcgtggcttg tgtcgatcac
     1981 acacctgcaa cgccttcttc cctggcagcg cttgctgcct attcgggcgg cagtacactc
     2041 aagaacgctc tcgcttctgg gatctctgcc atgggacatg tgcacgcagg agcgggtgag
     2101 gcgaccgctc aaatactaca ggactatctc aaacgattcc gaacgcaaaa acccatggtg
     2161 gctgacggta tcaagatcga aacgattcaa ggactggcgg aatacatcgt tgacaaatac
     2221 accggcgtcc tgggcggcga gaaacgtaaa cttccgggct ttggtcatcg ttactacagt
     2281 ctgtatcgaa aggatcctcg tgcggaatcg gtgatgcaaa tcacccggga aatggctctg
     2341 gatgctgact acgtgaattt ggcacaccat ctcgaagaat cactgaagat gcgcaaaagt
     2401 ccggggctct gtctgaatgt ggatggggtt attggcgtgg tgttgtcggc tctcggactg
     2461 ccgccgaaaa ctggcaaggc gctattcatc atcccaagaa cctttggaat tgtgagtcag
     2521 ctgttggagc aggaagcggg aagtttcttc cgcctcgcca atgaatccgt gctttatgtc
     2581 ggcccggaca caggtcggac attcgagtga gaaaagcttt atcaaaagct gaatcttaga
     2641 ttccagacaa cggaggtggg ccaatgccag cacagccatt cgaaggtttc tttcctgaag
     2701 aactcagcaa ggcgatccag acatatttcg agaagttcgg cctcgagtgt cacctgagag
     2761 agtgggttgt ctccaagatt cccggtatgg aagaattcga cgtgacaatg aagatccatg
     2821 ccaaccgaaa ggcctcggaa acccgcatca catagaaggg ttggaatctg atctgagaat
     2881 tgtgctatga atccgaaggg cgtgagagat caagccgtgt atttttgtgt ttggggtcaa
     2941 ctatatgcaa gtaaatgccg tatgtgtgag gaacctgagc aagcgctata acaaactctt
     3001 ggcgctgtcg gaactgaatc tcaccgttgc accgggagag gtctttggac tgatcggccc
     3061 caatggggca ggcaagacca cactgctgcg aatcctcggg actctttgcg aaccgaccgg
     3121 tggagaagct catgtattcg ggatcaatgt ccaggaacag ccaagagccg tccatgccat
     3181 gattggcttc atgcccgact tctacgccct ctatgacgat ctgaaggttt gggaatacct
     3241 ggactacttt gccggacttc atggaatcgc aagagaccaa cgcagaaaga aatgcgatga
     3301 gatgctcgcc agagtaaatc tggaagtcaa aagagatgca ttggtcgggg aaacctcccg
     3361 aggcatgaaa caacggctgt gccttgctcg tgccctgatt cacgaaccgc aactgctgct
     3421 gctggacgaa ccagcttcgg gactggatcc ccaagccaga cttgaattgc gcgatctgat
     3481 cagacaactg cagtccgaag gcaagacact catcgtatcc tcgcacatcc tcactgagct
     3541 ctccggattc tgcacgtcgt tcggacttct ggagaaagga gttctggtca agtctggcag
     3601 actggaagat attcagaagg agcagccgtc ccgtatcgtc tttctggatg tcctggagga
     3661 ctcagcccgt gtggcctcag tgctggcaca gttcccgagt ctttctgtca cgactcacga
     3721 agggcagcag tacgtcctgt ctctgacggg gcaaagagaa gacattgccc agttgcaccg
     3781 tcatctggtg caggcagaaa tccctgtggt ctc
//
LOCUS       DAOQUC010000201         3813 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_27089_length_3813_cov_2.816924, whole genome shotgun sequence.
ACCESSION   DAOQUC010000201 DAOQUC010000000
VERSION     DAOQUC010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3813
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27089_length_3813_cov_2.816924"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..932)
                     /locus_tag="PKH07_04740"
     CDS             complement(<1..932)
                     /locus_tag="PKH07_04740"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HNT34287.1"
                     /translation="MMNRSRVMVALILAFCASEALAQAVVQVPRIATSPLIDGRVTTA
                     EWGSAAKVDPFVDADSGTGVQAQTQVYLAHDGQTLYIAFRCFEPQMDQLQTVVADRDG
                     TLWNDDCVEVFLDVAGKRESFAHFIVNPIGAQYDAMGSDASWNCRWLALTGREQDAWT
                     VEVSIPVAELGVKSIKPADLWFGNFCRSRHAGSELSSWNRTGASFASPKSFGALIFDS
                     FSLRAASDNDVLLSALGRLTQELQGSPFVAPETLSEAKRLTQTLQQQSQAFGREWTRE
                     EYLQAQSNYDEAGEMVKRLEFAGKRRQLENRPYLL"
     gene            1130..3544
                     /gene="pheT"
                     /locus_tag="PKH07_04745"
     CDS             1130..3544
                     /gene="pheT"
                     /locus_tag="PKH07_04745"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HNT34288.1"
                     /translation="MKISWNWLKEFVEIDWTPQQFVDQMMMLGLEVESSESIGAAIEK
                     VIVGQIREIAPHPQAENLVVCQVDAGHGTDLNIVCGAKNMKVGDKVAVATHGTELPSG
                     LTIKKAKLRGITSEGMMCSDRELGLSDEHAGIKILAPDSPIGTPLPKVIGLDDTVLDI
                     KITPNRPDYLSLLGIARDVAAATHKTVKDVHHTLQESDRSAKELSSVQILDGELCARY
                     AARLIRGVKVAPSPDGLKTRLERVGQRSINNVVDATNYVLWELGHPMHAFDMDKLNEQ
                     RIVVRRASEGEKIRTLDGVERTLTSEMLVIADARVPVALAGIMGGADSEVSDETTNIL
                     LESAYFDPVSTRRTSKQLGISTEASYRFERGADYHAVITALDRCAAMIQRLAGGEIFK
                     DVIDTQAQQDMQLQTPRTVTLRRKRVTHVLGNEIATDDVVQLLTRLGFHLQPIEEDVW
                     SVQVPSFRVDVSQEADLIEEIARLYGYDRIVSTYPRLEVTPLERNTPYHLQRSLSDLM
                     VSAGLQEVVTYSFMAERDLDDLNLPQDSPLRQAFRLTNPLSQEESLIRTTLAPRLLKV
                     YDYNQRRARRDLAIYEIADTCRNNDGTLDEHAKLGILLSGEPSPHWSVPARERDFFDA
                     KGLVMLILNATRARNIRFQKSTSSFLNPERAMDMFFEGIPLGFFGELAPEIAEKMDFL
                     SRVSLIEINLDVLHQAAGEDQRKFVAPSPYPAIERDVAILVPLDAACDLILDAIRKTG
                     KAMLESVVLFDRYRGKQVDADRCSLAFRLVYRSPEKTLTEEEVNARHEKILKMLERDY
                     NAVLRG"
     gene            3571..>3813
                     /locus_tag="PKH07_04750"
     CDS             3571..>3813
                     /locus_tag="PKH07_04750"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="catalyzes the formation of
                     3-hydroxy-2-oxo-4-phosphonooxybutanoate from
                     erythronate-4-phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase"
                     /protein_id="HNT34289.1"
                     /translation="MRIIADKAIPFLQETFGTLGEVTALHSREITPDTARDADMLIVR
                     TRTRVNEHLLEGSRVRFVASAAVGTDHIDLEYLRTRG"
BASE COUNT          925 a         1020 c         1019 g          849 t
ORIGIN      
        1 agcagatacg gtcggttttc aagttgtctt ctctttccag caaactcaag tctcttcacc
       61 atttcgcctg cttcgtcata gtttgactga gcctgaagat actcttccct tgtccactca
      121 cgaccgaagg cctgagactg ctgctgcagt gtctgtgtca gccgtttggc ctcagacagc
      181 gtctcggggg caacgaaggg agaaccttga agttcctgtg tcagtcttcc gagagcgctt
      241 agcaacacat cattatcaga ggcagcacga agactgaaag agtcaaagat aagcgctcca
      301 aaggacttgg gagatgcgaa agaagctccc gtacgattcc acgagctcaa ttcggaacca
      361 gcgtgccgtg accgacagaa gttcccaaac caaagatccg ctggcttgat agacttcaca
      421 ccaagttccg caaccggaat cgagacctcg accgtccagg cgtcctgttc ccttcccgtc
      481 aaggcgagcc atcggcagtt ccaggaagca tccgaaccca tggcatcgta ttgtgcccct
      541 attgggttga cgatgaagtg tgcaaacgac tcacgcttgc ccgcgacgtc gaggaaaact
      601 tcgacacagt cgtcgttcca caatgtcccg tcacgatctg ccacgacagt ctgcagctga
      661 tccatctgag gttcgaaaca acgaaaggca atgtaaagtg tctgaccgtc atgagccagg
      721 tagacttgcg tttgtgcctg aacacctgtg ccgctgtcag catccacaaa cgggtcaacc
      781 tttgcagcag atccccattc ggcagtcgtg acacgcccgt caatgagagg ggacgtggcg
      841 attctcggca cctgcaccac agcctgcgcc aacgcttcag aggcacaaaa ggccaatatc
      901 agagcaacca tgactcggga tcggttcatc atgacttgtc tccttggcga taatcatcct
      961 aagacatggg cattctagcc aagagactgt ccacgtcaaa tggaggctcg tacgctgcag
     1021 tgatggtcgc cggatgtgcc gctgacgagt cgtgttgaca caacggctct tgggaacgta
     1081 tattctcgaa tctgttcaca ttgagaacaa ccatagaagg cagagtgaaa tgaagatcag
     1141 ttggaactgg ctcaaggaat ttgtcgaaat agactggaca ccgcagcaat ttgttgacca
     1201 gatgatgatg ctggggctgg aagtggagag cagtgagtcc atcggtgccg ccatcgaaaa
     1261 agtcattgtg ggacagattc gagagattgc cccccatcca caggcagaaa accttgttgt
     1321 ctgccaggta gacgcgggtc acggcacaga tctcaacatt gtctgtggtg ccaagaacat
     1381 gaaggtcggt gacaaggttg ctgtggcaac ccatggcacc gaactaccca gtggtctgac
     1441 catcaagaag gcaaaactcc gtggaataac gtccgagggt atgatgtgct ctgatcgtga
     1501 actgggtttg agtgatgagc acgccggaat caagattctt gctccggatt ccccaatagg
     1561 cactcctctg ccaaaagtga tcggtctgga tgacaccgtt ctggacatca aaatcactcc
     1621 caacagaccc gactatctgt cgctcctcgg catcgcccgc gatgtggccg cagccacgca
     1681 caagaccgtc aaggatgttc accatactct ccaggaatct gaccgttctg ccaaagaatt
     1741 gtcctcagtt cagattctcg acggcgaact gtgcgcacgg tatgctgcac ggctgattcg
     1801 aggtgtcaaa gtggcaccct cacccgacgg gctgaaaact cggctggagc gcgtcggaca
     1861 gagaagcata aacaatgtgg tggacgccac caactacgtg ctctgggaat tgggtcatcc
     1921 catgcacgcg tttgatatgg acaaactaaa tgagcagcga atcgtcgttc gtcgagccag
     1981 tgagggagaa aagataagaa cccttgacgg ggtcgagaga acgttgacat ctgagatgct
     2041 tgtgatcgcg gacgctcgag ttcccgtcgc gttggcaggt atcatgggcg gtgcggattc
     2101 ggaagtctct gatgaaacga ccaacattct tctggaaagc gcctatttcg atcctgtttc
     2161 aactcgacgc acttcaaagc agcttgggat ctcgactgag gcttcctatc gcttcgaacg
     2221 aggcgcagac taccatgctg tgatcacggc gcttgaccgt tgcgccgcaa tgatacagcg
     2281 gctggcaggt ggcgaaatat tcaaggacgt tattgacact caggctcaac aagacatgca
     2341 gttgcagacg ccccgaacgg tcactctccg ccggaagcga gtcactcatg ttcttggcaa
     2401 tgagattgcc actgatgatg tggtgcaact gctgacgcgc ttgggatttc atcttcagcc
     2461 catcgaagag gatgtctgga gtgttcaagt accttccttc cgagtggacg tctctcagga
     2521 agcagatttg atcgaggaga tcgctcgact ttacggctat gaccgcattg tcagtacgta
     2581 cccacgcctt gaagtcactc cacttgagag gaatactcct taccatcttc agcgttcact
     2641 ctccgatttg atggtcagcg cgggtcttca ggaagtggtc acatacagtt tcatggcaga
     2701 acgggatctc gatgacctga atctccctca ggattccccg cttcgccagg ccttcaggtt
     2761 gaccaatcct ctctcgcaag aggaatccct gatccgaacg actttggctc cgagactcct
     2821 gaaggtctat gactacaatc agcgaagagc tcggcgcgat ttggcaatct acgagattgc
     2881 ggacacgtgt cggaacaatg atggaacact ggatgagcac gcaaaactag gcatcctgct
     2941 ttcgggagag ccgtcaccac actggtcagt gcctgctcga gagcgcgact tctttgatgc
     3001 caagggtctg gtgatgttga ttctgaacgc aacccgagcg cgaaatatcc gatttcaaaa
     3061 gagcacttct tcattcttga atccggaacg tgccatggat atgttcttcg aaggaattcc
     3121 tcttgggttc tttggtgaac ttgctccaga gattgcagag aagatggatt tcctctctcg
     3181 tgtatcgctg atcgagatca atctggacgt tcttcaccag gctgcgggtg aggatcagcg
     3241 caagtttgtc gctccttcac cgtatcctgc catcgaacga gacgttgcca tcctggtacc
     3301 attggatgcc gcctgcgacc tgattctgga tgcaattcgc aaaacgggga aggcgatgct
     3361 tgagtccgtg gtcttatttg accgatatcg tggcaaacag gttgatgcag accggtgcag
     3421 cctggcattt cggctggtct atcggtcacc ggagaagaca ctgactgagg aagaagtcaa
     3481 cgcccgtcac gagaagatac tcaagatgct cgaacgcgac tacaacgccg tactgcgcgg
     3541 ctgaaatgcg ggcagtcgcc cagaaagcca atgagaataa tcgctgacaa agcaatcccg
     3601 tttcttcagg agaccttcgg gacgctgggc gaagtgaccg cacttcactc tcgggagatc
     3661 acccctgaca ccgcacgaga tgccgatatg ctcatcgtgc gaactcgaac acgcgtgaat
     3721 gagcacctgc tcgagggttc acgcgtccgg tttgtggcga gtgccgcagt gggaacggat
     3781 catattgacc tggagtacct tcgaacccgt gga
//
LOCUS       DAOQUC010000202         3798 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_27226_length_3798_cov_3.021640, whole genome shotgun sequence.
ACCESSION   DAOQUC010000202 DAOQUC010000000
VERSION     DAOQUC010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3798
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27226_length_3798_cov_3.021640"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="PKH07_04755"
     CDS             complement(<1..180)
                     /locus_tag="PKH07_04755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34290.1"
                     /translation="MRFFDCGVVIRRLPQRNQAFFVLLLVFLGVFDGTICGSEETGKM
                     ETLAQIYARLGREAQE"
     gene            1000..3174
                     /locus_tag="PKH07_04760"
     CDS             1000..3174
                     /locus_tag="PKH07_04760"
                     /inference="COORDINATES: protein motif:HMM:NF021507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HNT34291.1"
                     /translation="MRSLTGARGRLLVGLLLVGLFFSTIPGFSEEQVGPGLFYSEIVQ
                     QEMPWQIRVLRLDLNSPHLQLEGVLSQDGILSGLEKASHISRRLDREDAPVAAAVNAD
                     FWGAHRDPLGMTVIDGEIVRSPSERSVFVVKEDGTLLIDEFKLRCYIERDKATDTQVV
                     GINRWDNENGALLFNHWIGSKVPTTEQGAGWVLNPNGKKLESKCLIPCTVESAFKHEQ
                     GGTPMPSDRIVLWFPKGQDPGIQQGEEISIDMNLEPDYSPIKLAVGGGPRIVRDGEPV
                     AGDDNFSTVRHPRTAVGFSQDGKELILLAVDGRRAGWSIGMSLWELGQKMRELGCWQA
                     MNLDGGGSTTMVLWHEVKNRPSDNTGERSVANCLLVRSTAPRGKVEHISLKRAKVHLL
                     PASTFKMDIIGTDRFHQPVSIDDSTVRWSTPRGWFYFNRPLGTISKSGLLRAGSRPAE
                     GVVTAKVNGLETSVRVVVERPKWLQIEPEQLDLPLGRDKQITVTALGSDGIILSGTPG
                     DLQFTMKGDCGEVDQNGLFRPLKQGQGTVVVRLGGMTAEIPVNVSETYKVLLEDFEDC
                     SDWTVTKARADETQCGLIETREHLKFGDKTGKLFYKLERGGISCVYMETDKSWEGTAE
                     AAEIWIYGDGSGHMLRAELFDDQNNVFLVDLVSAVDWSGEWKQVHVSLLNPAPHWSNP
                     TAKMGRLCRWKNIYLAETKEDKKNSGAIFFDDLEVITVPPAK"
     gene            3224..>3798
                     /locus_tag="PKH07_04765"
     CDS             3224..>3798
                     /locus_tag="PKH07_04765"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNT34292.1"
                     /translation="MAFLEIIVVNWNGLNLLKQCIESLQRQTCGDFTMTVVDNGSNDG
                     SVAWLQTNHPSVKLIPLDDNTGFARANNLAALQSNADYLLLLNNDIEVEPNFIDAICC
                     GLQKHPDADMASVCMIQHHRRDRVDNLGMDWTWVGVPRQLGHGFAVEKISREEKFIFG
                     PSAGAGVYRRDLFLKLGGFDETFFAYCEDSDL"
BASE COUNT          866 a          938 c         1097 g          897 t
ORIGIN      
        1 ttcctgggct tctcttccaa gtctggcgta aatttgtgcc aatgtttcca tttttcctgt
       61 ctcctccgat ccgcaaattg tcccatcaaa gacccccaga aagaccaaga ggagaacaaa
      121 aaaggcttga tttcgctggg gtaaacgccg aatcacgaca ccgcaatcaa aaaaacgcat
      181 accagttcct cggatttgcg actccaaagg gagtcatcgt ccaactcagt ccgctgcacg
      241 ctatgcaaag gtgtagccat ctacaggact ccacgaatct atcacttgcc accctcggga
      301 acaaccgatt gggtggtttc cgttcttttt ccttgacaca aaccgaaaaa aagggtgata
      361 atcagaacga taacgaaggc tgggatgtga gttcttggtt agtctaggat gaggaggtta
      421 tgcctgtgat gatcatcgtt aggtaccttc ttcctggcct gctatggttc atctccattt
      481 gtacgggcgg tatgctggtg ttctggaaac tgttcctgaa ctgaggatga tggatttacc
      541 agaagaaggt acggcagaag catcgaattt ccagagtgtc cccgtgcggc ggggttcgct
      601 ccacaagccg gtaacatctc cccgggtgta gggagttctt tttcattttt tcgatattcc
      661 tcctcaaata agtcatactt aactccaaat atatcagtga atagcatgga tttgcagacg
      721 cgagcgagtg atgcaccatg cgtcctgtca gagtctttgc tcctagtgac acaaagtccg
      781 aacggcacgt gtcatttatt gagcaagcgt ccgaaggagt gggcatagcc cacgcgcctg
      841 caggcgttgg cctgctcgga ctgccttcga gttgtggagt ggatgctccg gccagcaggt
      901 tcagtttgtc cagagaatcc tctaccaaca gaggcattct ggtttgccaa gccgtctgtg
      961 ccgcgctatt gtcctgaaaa acagcaagga gatatacgca tgcgatcatt gactggagca
     1021 agagggcgtc ttttagtcgg cttactgctt gtcgggcttt tcttctcgac gattccgggt
     1081 ttctcggagg aacaagttgg gcctgggctg ttctactcgg agattgtcca gcaggagatg
     1141 ccctggcaga ttcgagtgct gcgtttggat ttgaactcac cccatctgca actggagggc
     1201 gtactctctc aggacggcat cttgtcgggt ctggagaaag ccagccatat cagccgccga
     1261 ctggatcggg aggatgctcc tgttgccgct gcggtgaacg ccgatttctg gggtgcgcat
     1321 cgcgacccac tcggaatgac ggtcattgac ggggagatcg tgcggtctcc atcggagcgc
     1381 tctgtgtttg tcgtcaagga agatgggact ctcctgatag atgagttcaa actccgctgt
     1441 tacattgaaa gggataaggc aaccgatacc caggttgttg gaatcaaccg ctgggacaat
     1501 gagaacggag cgcttctgtt caaccattgg atcggttcaa aggtaccgac gaccgagcag
     1561 ggagcgggtt gggttctgaa cccaaacggg aagaaactcg aatcgaaatg cctgattccc
     1621 tgtaccgtgg aaagcgcgtt caagcatgaa cagggtggaa cgccgatgcc ttccgaccga
     1681 attgtccttt ggtttcccaa aggtcaggat ccgggaattc aacagggtga agagatttcg
     1741 atagatatga acttggaacc cgactacagc ccgatcaaac ttgctgttgg tggaggccct
     1801 cggatcgtcc gtgatggaga gccggttgca ggcgatgaca atttcagcac ggttcggcat
     1861 ccccgaaccg cggttggctt ttctcaggac ggcaaggagc tgattcttct ggcggtggat
     1921 ggacgccgag ccggctggag cattgggatg agcctttggg aactgggtca gaagatgcgt
     1981 gagctgggct gctggcaggc tatgaatctc gacggcggcg gttccacaac gatggtcttg
     2041 tggcacgaag tcaagaaccg cccatccgat aacaccggag agcgctctgt tgccaactgt
     2101 cttttggtac gaagcaccgc cccccgaggc aaggtcgagc acatctccct gaagagggcc
     2161 aaagtgcatc tgttgccagc cagtaccttc aagatggata tcatcggcac ggatcggttc
     2221 catcaaccag tttcgataga tgactccacg gttcgctgga gtacgccacg cggctggttc
     2281 tatttcaacc gcccccttgg aacgatctcg aagtccggtt tactccgcgc cggttcccgt
     2341 ccggcagagg gggttgtcac agcaaaggtg aacggtctgg aaacgtccgt tcgggttgtg
     2401 gtggagcgtc caaagtggct tcagatcgaa ccggagcagt tggatctccc cttggggcgt
     2461 gacaagcaga tcacggtcac cgcgcttggc agtgatggaa tcattctttc tggaacaccc
     2521 ggcgatctcc agttcacgat gaagggcgac tgtggggaag tggatcagaa cggtctgttt
     2581 cgaccgctca agcagggaca ggggactgtc gttgttcggt tgggcggcat gaccgcggag
     2641 attcccgtca atgtcagtga gacatacaaa gttttgttgg aggactttga ggactgctcg
     2701 gactggacgg tcaccaaggc acgtgccgac gagacacagt gtggtttgat cgagactcgt
     2761 gagcatctca aattcgggga caagacggga aagttgttct acaaactgga acggggtggc
     2821 atcagctgcg tctatatgga gactgacaaa agttgggaag gaaccgcgga agcggcggaa
     2881 atctggatat acggggatgg cagcggtcac atgcttcggg cggaactttt cgacgatcag
     2941 aacaacgtgt ttcttgtgga tttggtttcg gcggtggact ggtctggaga gtggaaacag
     3001 gtacacgtgt cgcttctgaa tcccgctccg cactggagca atccaacggc caagatggga
     3061 cgcctctgcc gatggaagaa catttatctg gcggagacca aggaagacaa gaagaacagc
     3121 ggtgcgattt tcttcgatga cctggaagtc atcacggtgc cgccagcgaa atagcaggga
     3181 actggcgcgt tcacatcggt cgcacctgct gccaataatg tccatggctt ttcttgaaat
     3241 cattgtcgtc aactggaacg ggctgaatct gctcaaacaa tgcatcgagt ctttgcagcg
     3301 gcagacgtgt ggggatttca ccatgaccgt tgttgacaac ggctccaacg atggttctgt
     3361 cgcgtggctt cagactaacc atccttcggt caaactcatc cctttggatg acaacacggg
     3421 gtttgccaga gccaataatc ttgcagcgtt gcagtccaac gcggactatc tactcctgtt
     3481 gaacaatgat attgaggttg agccgaactt cattgacgcc atctgttgcg gactgcagaa
     3541 gcatcctgac gcagacatgg cttctgtctg catgatccag catcacaggc gtgatcgcgt
     3601 tgacaacctg ggcatggact ggacttgggt gggagtcccc cgacaattgg gacacggctt
     3661 tgctgtcgag aagatttcgc gcgaagagaa gttcattttt ggaccgtccg caggggcggg
     3721 agtctatcgt cgcgatctgt ttctcaaact tggcggtttt gatgaaacct tctttgccta
     3781 ttgcgaagac agcgatct
//
LOCUS       DAOQUC010000203         3797 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_27236_length_3797_cov_3.040620, whole genome shotgun sequence.
ACCESSION   DAOQUC010000203 DAOQUC010000000
VERSION     DAOQUC010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3797
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27236_length_3797_cov_3.040620"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1970
                     /locus_tag="PKH07_04770"
     CDS             <1..1970
                     /locus_tag="PKH07_04770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34293.1"
                     /translation="ERYLQQAEEVLRTYSDEIWGVFTGDEITHYQTYGLIKAVSEQSS
                     LHPYAQAIDRDVRDRFGLGQYGVPRSVEDQEPYRWMAVEKWIDDQVCLLTKDLRKVVD
                     RVAPQMLLVSDDPEAGFHGHDYARRGKWCDIVTHQTYPSRSPMRQEVGFITKMLFDLS
                     GKSVWPCVHAENYGASYSPEETAEVYSQVFRCGGDGIHYYPDDVQGTRRNILDTHYCR
                     IGAPDRWATMQAIFERVRDLPRLKLPRKTRTAIFIEPSTYQATLPTKRGQHLEYEMCF
                     SFLGPNARSWFQFVHAGQVARNEVKLSQHRTVFLPMAKFFDPEMAQSLIEFCKKGGTV
                     ICGDPEVFRYSTQQAYQPVPALNELGWKCSDKTSPVDCIRILRNDLWRNIPNGTTISF
                     PNPLQAFVIEPRDSFETLAHFDTGTPAIVCRKLGKGNWVQFAFQPFTYECLCNETMRT
                     LFRSILERQGEKTGLDIWRFTFPVAESPKPPKGQCLTGNACVWEMNVPRNVGGSGIPI
                     TVRWTTPPQGVNEKTVSSDACRLFDRRKALTAQNDRQISSALENWTYIWETQNSFNVE
                     LELNATHTLDRLQVWFSGALPKADVLIHTKGDVWSRLGAFDAMDVGEDVAMITVSLEN
                     AEADCVRIVFAPSSDSNRFSLCELEVWAPSK"
     gene            complement(2049..3233)
                     /locus_tag="PKH07_04775"
     CDS             complement(2049..3233)
                     /locus_tag="PKH07_04775"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNT34294.1"
                     /translation="MRLLFVTPVFPGQAYGRRPYNFIKVLAPKHSVDLICASAKPISQ
                     AEIQAIKDMGVRTVTIVSHPIWKGALSALGALPRRHPLRAGYCESGSMSRAVADFLEH
                     HQVDLMHIDRSRMARLATLRPRNTKCVVDFTDAMTLLFERKIQYTRNPLSRAICLWER
                     QTQPRFEQQIAPTFDGCLLCSPVDAKVVEKTVPPEKIHLIPNMVDTDEFTVKRHDGSP
                     RLVFTGTWTYFPNTDSLAWFTETILPRIRNVFPSLETQVIGARPGKSVLRLNGRNGIQ
                     VIGAVERMADHLFADDVFVCPLRVGAGVRNKILEAMACGMAVVSTTLGAEGIPVEDGL
                     DIMLADDPQAFAEKIVTLLKNQPMRMQLGAQARKTIIQHHSEATVAPQIENLYESIVA
                     SR"
     gene            complement(3320..>3797)
                     /locus_tag="PKH07_04780"
     CDS             complement(3320..>3797)
                     /locus_tag="PKH07_04780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34295.1"
                     /translation="ILAHLGFYSLLALPKQQLGATSFLRNSIALAALTLALPVLAKRL
                     VSNLGEETDRGIQWMHGFAALLLADWLFVTRTGAIGNYITALWAVSAIVIVLIGLADR
                     KRPFRIVGLIGLLACVPRVFLVDVHSMLYRIAAFGALALVTLGVAFLYSKYKHLIE"
BASE COUNT          927 a          991 c         1028 g          851 t
ORIGIN      
        1 gagagcgata tctgcaacag gcggaggaag tgcttcgtac ctacagcgat gagatttggg
       61 gagtgttcac tggagatgag atcacccact atcagaccta cggccttatc aaagctgttt
      121 cggaacagtc cagtctgcat ccttacgctc aagcgattga tcgggatgtg cgtgaccgat
      181 ttggtcttgg gcagtatggc gtgccacgat cggtggaaga ccaggaacct taccggtgga
      241 tggcagttga gaagtggatt gatgaccagg tgtgtcttct cacgaaagat cttcgcaagg
      301 tcgtcgatcg agtcgcaccg caaatgcttc ttgtcagtga cgatcccgag gcgggtttcc
      361 acggacatga ttatgcccgt cgggggaaat ggtgcgacat cgttacgcac caaacctatc
      421 catccagaag tcccatgaga caggaagtcg ggttcatcac caagatgctt ttcgatcttt
      481 caggcaagag cgtctggccg tgtgttcacg ctgaaaacta tggggcatcc tactctccgg
      541 aggagacagc ggaggtctac agccaggttt tccgatgcgg tggagatggt attcactact
      601 atccggacga cgtccaggga acccgcagaa acattctcga tacgcattac tgtcgcatcg
      661 gtgctccaga tcggtgggcg actatgcagg cgattttcga acgcgttcgc gatctgccac
      721 gcctgaaact gccgcgcaag accaggacag cgatcttcat cgaaccttcg acctatcagg
      781 caacgctccc aaccaagcga ggtcagcatc tggaatatga gatgtgcttt tcgtttcttg
      841 gtcccaatgc gcgatcttgg tttcagtttg tgcatgcagg acaggttgcg cgcaatgaag
      901 tcaaactttc ccagcaccga accgtttttc ttccgatggc gaaattcttt gaccctgaaa
      961 tggctcagtc acttatcgag ttctgcaaga aaggtggcac tgtgatctgt ggcgatcccg
     1021 aggtgtttcg ctattcgact cagcaggcct atcaaccggt tcctgcactg aacgaactgg
     1081 gttggaagtg ctcggacaag acttctccag tggactgtat caggattctc agaaatgacc
     1141 tctggcgaaa cattcccaat gggacgacaa tatctttccc aaatcctctg caggcatttg
     1201 tgattgaacc gagagactca tttgagaccc tcgcacactt tgataccggc actcccgcga
     1261 ttgtttgccg aaaactgggg aagggcaact gggtacagtt tgcttttcag ccgttcacgt
     1321 acgaatgcct gtgcaatgag acgatgagaa cgctgtttcg gagcatcctt gaacggcagg
     1381 gagagaagac gggcttggat atctggcggt ttacctttcc cgttgctgag tctccgaagc
     1441 ctccaaaggg acaatgcctt accggaaacg cttgtgtatg ggagatgaat gtgcctcgaa
     1501 acgttggtgg atcgggtatt ccgattactg ttcgctggac aactccccca caaggcgtaa
     1561 acgagaagac ggtgagttca gatgcatgca ggctctttga ccgtcgaaag gcacttaccg
     1621 cacaaaacga caggcagatt tcctctgctc ttgagaactg gacttacatc tgggagacac
     1681 agaattcctt caacgtggaa ctggaactca atgccacaca cactctggac agacttcagg
     1741 tgtggttctc tggagcactt cccaaggccg atgttcttat tcataccaag ggagacgttt
     1801 ggtcgagact gggcgcattt gacgccatgg acgttggaga ggacgttgca atgattacgg
     1861 tgtctctgga aaacgcagag gccgactgcg ttcggattgt ttttgcgcca agctcggatt
     1921 ccaaccgctt ctcactttgt gaactagaag tgtgggcacc aagcaagtag tccttgctca
     1981 ccgccattgt cgaagcagac gaacacgctt tcgggttccc gaaaccacag gaagcactgc
     2041 ccagatcgtc agcgtgacgc gacgatgctt tcatagagat tctcgatctg aggagcgact
     2101 gtggcctctg aatgatgctg gatgatcgtc ttgcgtgcct gagcacccaa ctgcatccgc
     2161 atcggctgat tcttgagaag ggtgacaatc ttctccgcaa atgcttgagg gtcatctgca
     2221 agcatgatgt ccagaccatc ttcgactgga ataccctcag caccaagagt cgtggagaca
     2281 acagccattc cacaagccat ggcttcgagt atcttgtttc gcactcccgc tccgacgcgc
     2341 aagggacaga caaagacgtc atcagcaaag agatggtcgg ccatccgttc gaccgcaccg
     2401 atcacctgaa ttccattccg accattgagt ctgagaacgc tctttcctgg ccgtgctcca
     2461 atgacctgcg tctccaaact cgggaacacg tttctgatcc gaggaagaat tgtctcggtg
     2521 aaccaagcca gagagtcagt gttggggaag taagtccatg ttcccgtaaa caccagccgc
     2581 ggagatccat cgtgcctctt gaccgtgaat tcatcggtgt caaccatgtt cgggatcaga
     2641 tggatcttct ccggtggcac tgtcttctcc acgacttttg cgtccacagg agaacagagc
     2701 agacacccat cgaacgtggg agcaatctgt tgctcgaaac gaggctgggt ctgtctttcc
     2761 caaagacaga ttgcacgact cagcgggttt cgggtgtact ggatctttcg ttcgaaaaga
     2821 agcgtcatcg cgtcggtgaa atccaccaca cactttgtgt ttctgggtcg aagcgttgcc
     2881 agtcgagcca tgcgcgatcg gtcaatgtgc atcaggtcaa cctgatgatg ttccagaaaa
     2941 tcggcaactg ctcgactcat ggaaccggat tcacagtagc ctgccctgag gggatgccgt
     3001 cgtgggagcg ctcccaaagc agagagggca cccttccaga tcgggtggga gacgatcgtg
     3061 accgttcgaa ctcccatgtc tttgatggct tgaatctcgg cttggctgat tggcttggcc
     3121 gaggcacaga tcaggtcaac agaatgcttt ggggcaagca ccttgatgaa gttgtaggga
     3181 cggcgaccat acgcctgacc aggaaaaaca ggcgttacaa agagcaacct catcgcagca
     3241 aaaactcctt ttctcaaaac aacatcaccg aaatcatacc atgggtcacg agcacagacc
     3301 aatgagagca gagacggctc tactcaatca agtgcttgta cttgctgtac agaaacgcca
     3361 cgccaagcgt caccagagcc aaagccccaa atgcggcgat gcggtacagc attgagtgaa
     3421 cgtccaccag gaagactctt ggaacacatg ccagcagacc gatcaagccc acgatgcgaa
     3481 agggtctctt gcggtcggcc agtccaatca gaacaatgac gattgcggac acggcccaga
     3541 gggcggtgat gtagttgccg attgctccag tgcgggtgac aaagagccag tctgccagaa
     3601 gcagcgctgc aaagccgtgc atccactgaa tgcctcgatc ggtttcttca cccagattgg
     3661 agaccagcct cttggccagg acaggcagag ccaatgtgag cgctgcaagg gcaatagaat
     3721 tgcggagaaa tgaagtcgct ccgagttgct gtttaggcaa agccagaagg gagtagaatc
     3781 ccagatgcgc tagaatc
//
LOCUS       DAOQUC010000204         3783 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_27407_length_3783_cov_2.770923, whole genome shotgun sequence.
ACCESSION   DAOQUC010000204 DAOQUC010000000
VERSION     DAOQUC010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3783
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27407_length_3783_cov_2.770923"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..388
                     /locus_tag="PKH07_04785"
     CDS             <1..388
                     /locus_tag="PKH07_04785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34296.1"
                     /translation="APDGEQVLFISTLNNYASFWRIRLDGSGLEQLTNFDPDGKRDEL
                     IPVAFSRSLSWHPSGAFLIYDTTDPGGGHSIWLFDPTGKRAQRIQQGRWPKLLSSGKT
                     IAFETPEGGISAMTLPEFVRKQEVQR"
     gene            385..2634
                     /locus_tag="PKH07_04790"
     CDS             385..2634
                     /locus_tag="PKH07_04790"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HNT34297.1"
                     /translation="MSIRILPILSGVTLLMVATPILAATPGGPLGCPFSDFDVWRVTS
                     AFDLDPTSAVMADWMGWRTGEPTAGSGHAYDGHTGTDWGMPNGTELLAVLDGTVSSWR
                     DGVPNDDHSDTGNYIILNHTTARGNFRTNCWHLSQGTVVPRSVGAAVTKGSKIAESNN
                     TGNSTGPHLHFGVAYTSGSAAYSCGFYNGWFEDDVFYYGDTRACLRYVQVNTSALNCR
                     VGNTTGYDVITTLPLNSVFVASQRNGWYRIFLPMPPARAHESRTAAGAVSPSPGYVES
                     GSWSSGTEKSTVADAAGNVDRVTLAGAGSRFCDGVGTDTAQYVPNFTQRGQYEVFATW
                     PSNANAAGVQYTVRDLTGTYNVSLDQRGAFGTSGSGTKASPFRISQTPFTHSYTTVGG
                     EDTWDVYTPVGSGISQAGPEVLYQVTVRNPCTITVTVNHTGYPTLDVDIHLLSSLSNT
                     ACVARADWTFTYSIPSAGTYYIAADTYQNDSRATAYTLDVTVQGDPTFPNSWNSLGTY
                     SFARGQNASTGSITLDESTVTGVVEAGQPMRVYSDAIKLVPKITHRSGWVSDSYLTVL
                     GTAPCSVGVLVDRNAQNDSNVMDNYVEVPIYDSPNTGAGVVAKAVTGQRYVATRHVEG
                     DWYLIHLTNGAGATEGWIQGSNLFVYNDLPTETPAATATPTLTATQTPTRTPTQTPTV
                     TPTRTPTFTPTRTPTFTPTWTPTRTPTRTPTQTPSRTPTPTGVPTNTPMVTPTPVTSH
                     AGESWVLFR"
     gene            2787..>3783
                     /locus_tag="PKH07_04795"
     CDS             2787..>3783
                     /locus_tag="PKH07_04795"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HNT34298.1"
                     /translation="MSRWSSLKGALLSILSPISVLSMVMAFSVCAQEPTPKPNVVLIL
                     VDTLRADHLGCYGYQHNVSPNIDRLAAQSKVFTRCYSVAPWTLPSMVSIITSLYPEVH
                     GVKTEKDRLGDKGTVLAEVLRGHGYSTGAVVTSSFLTGTWNFDRGFDFFSKKPASGHS
                     GVTSAAATYHGRKFLKEQAKTPFFLMMHYFDPHTEYIEHGKFDFTSALGYKGPLEQGM
                     PIIDVWPLINKKLMTDDDTAFLKALYDSEIAFTDACIGGLLETLKEMGVFENTLIILT
                     SDHGEEFMDHGGLEHSRTLYEELIHVPLIIKMPNSSQPESLSIVVSVLDVFPTALD"
BASE COUNT          851 a         1045 c          976 g          911 t
ORIGIN      
        1 tgctcctgat ggggaacagg tgcttttcat ctccaccttg aacaactacg cttccttctg
       61 gcgcattcgg cttgatggtt caggccttga acaattgacc aactttgatc ccgacggcaa
      121 gcgagacgaa ctcattccag ttgctttcag ccgttccctt tcatggcatc cgtcaggggc
      181 atttctcatc tatgacacga ctgatccagg gggtggccat tcgatctggc ttttcgatcc
      241 gactggcaag agagctcaaa ggattcagca aggtcgctgg ccaaaactac tgtcttctgg
      301 caaaaccatt gcttttgaga ctcccgaagg agggatttct gcaatgacac ttcctgagtt
      361 tgtgcgaaag caggaggtgc agcgatgagc atcagaatcc ttcctattct gagcggagtt
      421 accctcttga tggttgccac gccgatactg gcagcgacgc ctggagggcc attgggttgt
      481 cccttttcag actttgatgt ctggcgcgtc acttccgctt tcgatcttga tccaacttcc
      541 gctgtcatgg cggactggat gggttggaga acgggagagc caacggcggg atccggccat
      601 gcctatgacg gacacacggg aaccgattgg ggaatgccca atggaacgga actgctcgcc
      661 gttctggatg gaacggtctc ttcatggcgg gacggtgttc ccaacgacga tcattctgac
      721 actggcaact acatcatttt gaaccataca acagcgcgtg gcaattttcg gacaaactgt
      781 tggcacttgt cccaaggtac cgtcgtccca aggagcgttg gtgccgcagt gaccaaggga
      841 agcaagattg ccgaatcgaa caataccggc aattctaccg gcccccatct gcattttgga
      901 gtggcttaca cgagcggctc tgcagcttac tcctgtggct tttacaacgg atggtttgag
      961 gatgacgtgt tctactatgg agacacccgt gcttgtcttc ggtatgttca agtcaacaca
     1021 tcagcactca actgccgagt tggaaacaca accggctatg atgtgatcac gacactaccc
     1081 ctgaattcag tctttgttgc ctcacaacga aatggctggt accggatctt tctgcccatg
     1141 ccacccgcac gagcccatga gagtcgcacc gcagccgggg ctgtttcgcc ttctccgggc
     1201 tacgtcgagt cgggcagttg gagttcaggc acagaaaaaa gcacggttgc tgacgcggct
     1261 ggaaatgtag atcgggtaac attggccgga gccggcagcc gcttctgtga cggcgttggc
     1321 acagacacgg cgcagtatgt ccccaatttc acgcaacgag gccagtatga agtctttgcc
     1381 acatggccga gcaacgccaa tgcggcgggc gtgcagtata ctgttcgcga tctcacaggc
     1441 acatacaatg tttcactcga tcaacggggt gcctttggca cttctggcag tggtaccaaa
     1501 gcatctcctt tccgaatcag ccagactcca tttacacaca gttatacgac tgtcggagga
     1561 gaggatacct gggatgtcta tacacccgtc ggttccggaa tcagtcaggc aggtcctgaa
     1621 gtgctctacc aggtcacggt tcggaatcct tgtaccatca cggtgacggt caaccataca
     1681 ggctatccga cgcttgacgt ggacattcat ctgctttcga gcctttcaaa cactgcgtgt
     1741 gtcgctcgcg cagactggac attcacctat tccattccgt cggcaggaac gtactacatt
     1801 gccgccgaca cctatcagaa tgactcccgc gccacagcgt acactctcga tgtgaccgtt
     1861 cagggagatc cgactttccc caacagttgg aacagtctag ggacgtattc cttcgcgcga
     1921 ggtcagaatg cctcgacagg ttcaattacc cttgatgaat ccacggtgac cggtgttgtt
     1981 gaagcgggac agccgatgcg cgtttacagc gatgcgatca aactggttcc gaagattacg
     2041 catcgttcgg ggtgggtttc cgacagttac ctgactgtgc tcgggaccgc accatgctct
     2101 gttggcgtat tggtggatcg caacgcccag aacgactcca atgtgatgga taactacgtg
     2161 gaagtgccga tctacgattc gcctaacaca ggggcggggg ttgtcgccaa agccgttaca
     2221 gggcaacgtt atgttgccac acgacatgtc gaaggggact ggtatctgat tcacctcacc
     2281 aacggcgctg gtgcgacaga gggatggatt cagggcagca atctgttcgt gtacaacgat
     2341 ctgcccaccg agacgcctgc ggcaaccgct actccgactc ttacggcgac acagacccca
     2401 acacgcactc cgacgcagac acctacggtc acgcccactc gcacaccgac ctttacacca
     2461 acgcggacac ccacgttcac tcccacatgg acgccgacac ggactcccac tcgaacaccc
     2521 acgcagaccc cttcgaggac tccaacaccg acgggagtgc caacgaatac gccgatggta
     2581 actccgacac ccgtgacatc gcatgcagga gaaagctggg ttctgtttcg ataggtcttt
     2641 ctttttggcg ctctgagcga gactcgctga tttgacactt gctgcgggct cgtgttctac
     2701 tgatcacgtt tgaacaatct gtcctgacgc agttactctt ttggatctgc agccgcgttc
     2761 agtgacatga tctcggagtt ggtacgatga gtcgttggag ttctctgaaa ggtgctcttt
     2821 taagcatcct gtctccaatc agcgttctct caatggtgat ggctttcagt gtctgtgcac
     2881 aagagccgac tcccaagccc aatgtggttc tcattctcgt ggacacgttg cgtgcggatc
     2941 atcttggatg ctatggatat caacacaatg tttcgccgaa cattgaccgg ctggcggcac
     3001 agtcaaaggt tttcacccgc tgctacagtg tggcaccttg gacccttcct tcaatggttt
     3061 ccatcatcac ctcactgtat cctgaagtcc atggcgtcaa aacggaaaag gatcgccttg
     3121 gggacaaggg tacggttctg gcagaggtcc tgcgaggaca cggatactca acgggtgccg
     3181 tagtcacaag ttcctttctg acgggtacct ggaatttcga tcggggcttt gatttcttca
     3241 gcaagaaacc tgcttcagga cactccggtg tcacttcggc cgccgccact tatcatggaa
     3301 ggaagtttct gaaggaacag gcaaagaccc ccttctttct gatgatgcac tatttcgatc
     3361 cacacactga gtacatcgaa cacggtaagt tcgattttac ctccgccctt ggctacaagg
     3421 gaccgcttga gcaagggatg ccgatcattg atgtctggcc tctgattaac aagaagttga
     3481 tgacagatga cgatacagcc tttctgaaag ccctttatga ctcagagatt gccttcacag
     3541 atgcctgtat cggcggactt cttgaaacac tcaaggagat gggtgttttt gagaacacct
     3601 tgatcattct cacctcagac catggggaag agttcatgga tcatggcgga ctggagcatt
     3661 cccgaacttt gtacgaagaa ctcattcatg ttccattgat catcaagatg cccaattcca
     3721 gccaacctga gtctctgtcc atagtcgttt ctgtccttga tgttttccca actgcattgg
     3781 atg
//
LOCUS       DAOQUC010000205         3778 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_27442_length_3778_cov_3.708837, whole genome shotgun sequence.
ACCESSION   DAOQUC010000205 DAOQUC010000000
VERSION     DAOQUC010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3778
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27442_length_3778_cov_3.708837"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            188..361
                     /locus_tag="PKH07_04800"
     CDS             188..361
                     /locus_tag="PKH07_04800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34299.1"
                     /translation="MELADSFHPTLLAEWELRVDRFRVFYDVDVGAKQVKILAVGLKR
                     GSCLSIRDKEFKI"
     gene            358..603
                     /locus_tag="PKH07_04805"
     CDS             358..603
                     /locus_tag="PKH07_04805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34300.1"
                     /translation="MRTVNIPKRSRKLFGLLEQAKHENLVLRSEDGTEFILAEIDDFN
                     REIELTRQNSDLIRYLETRARETKTIPLAEARTQLDL"
     gene            785..1924
                     /locus_tag="PKH07_04810"
     CDS             785..1924
                     /locus_tag="PKH07_04810"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HNT34301.1"
                     /translation="MVKVLHTADIHLDMPFRWLGQQGEVRRRGLRQLFGRLCQTVKER
                     ADLLVLVGDIFEHEYVTPDTLNFIKKQLSALAPKKVFIAPGNHDPCLKDSPYLLEKWP
                     ENVHVFRTNTFETVPVPEHRLTVHGIANTAFQDNNRYLQNYRIPKDGNLHVVLFHGSD
                     IQSMPPQYEQDAWFPFDEKDLLECGAHYVALGHYHSFRCIPSDEHNPKGCYPGCPECL
                     DRSDSDDKIALLVEVSEAGNRIEKVLLAEYGNDQVTLDCKGVSTREEIEQKIQALAKA
                     GNWSKKTVQVILRGEIDSGLDLRVDEIREAVRDVAFFLQIQDQTEFPYDLGQLASRRD
                     ALGEFVRRMKVQVEQGGLAEEDMRRTRLALLLGIDAFMRNEVRKP"
     gene            1921..>3778
                     /locus_tag="PKH07_04815"
     CDS             1921..>3778
                     /locus_tag="PKH07_04815"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNT34302.1"
                     /translation="MIFEDLLIRGFGNLSNVKFEFGQGLNVFVAPNEAGKSTLAECLF
                     RCLYGFPPLRSDEERAAYKRFQPLNGDRYSAKLRIATDEQTHYELDRDFAARTLRIRN
                     AVTGEDVTGQFPVEKKGQDVLLAQNLLGISRDTFRTIAFLAQGLVDRLENGQELSDEI
                     RKIADSGEFQTGYKKALEKLKKASDTIGKGERASSKPLWEAQERVNTLQREREEICAK
                     RKERITALERIEILKHQLAEADRACLELEVRGLALQQARVRATIGQLETLGREIDQAR
                     AIAQQLDGYRNVPCHRRDELFVNADRLKGVQQRLAQYQSQLASLDAELSAINDRLSET
                     EHLAKLSEKDIEQMDVLENALKRAETELDLLEGKISQSRLTQKRLEDQINALSQRLKG
                     FTGELGKDPLAFEQQLARYDQASSLEREVALRREQVSKTQARIAGKKGGFRWIALGAG
                     LFLFIIVAALKAAPGGEFVPAIIWGVTAGVAGFLFSNLGFRLVMKGDAEQLILEQETL
                     RTAEVELAKVEKERQQQLSNASVSNLKELRELFQLYRSHQSELENHQTLLQHLLNESG
                     RHEQELTSAQSQIQDYLMQCKIVAKDEPFQDKSLLRLRQQYLARKENCQKRDN"
BASE COUNT          943 a          919 c         1087 g          829 t
ORIGIN      
        1 agtcgagtag ggtggaacga gtctgcgagt tccaccactc cgaatgtttt caccggtcgg
       61 atgatctcgc ttgcatacag atatcaccga tgccattctg catttatggg cgcgcaagtc
      121 ttacggaagt aacaggatgg atggttgaca accgcatcgc tgaggaatgt gatagcgttg
      181 gaagaaggtg gaactcgcag actcgttcca ccctacgctg cttgcagaat gggagttacg
      241 tgtggatagg ttccgcgtgt tctatgatgt ggatgttgga gcaaagcagg tcaagattct
      301 agccgtaggt ctgaagcgag ggagttgtct ttccatcaga gacaaggagt tcaagatatg
      361 agaaccgtga atatacccaa gcgttctcgg aagcttttcg gattgttgga acaagccaag
      421 catgaaaacc ttgttcttcg ctccgaagac ggaacggagt tcatccttgc agaaatagac
      481 gatttcaacc gtgaaattga gctcacgcgc cagaacagcg atttgatcag gtatctggaa
      541 acgcgcgcca gagaaacgaa gaccattcct ttggcggaag cgagaaccca actggatcta
      601 tagcgcgttg gactgccttc cttgggctgt tactcgcagg caggatacgt tttaccgaat
      661 cctggagtcg gggactccag tctacaactg caactttgct accacactca tgccgcaaat
      721 ccgttgtccc gttatgatat tgctgatata ttagtctaca catcagagat ttgaggggca
      781 tgagatggtc aaagttctac atactgccga cattcatctg gatatgcctt tcaggtggct
      841 gggacagcag ggagaggttc gccggcgtgg gcttcgacag ctgttcgggc gtctgtgtca
      901 gacggtgaaa gagagagccg atttgctggt gcttgtggga gacatcttcg agcatgagta
      961 tgtcaccccc gacacgctca acttcatcaa gaaacagctg agcgcgctgg caccaaagaa
     1021 agtgttcatc gcacccggta atcatgatcc gtgcctgaag gattccccgt atcttctcga
     1081 gaaatggcct gagaacgttc atgtattcag gacgaatact ttcgagacag tgcccgtccc
     1141 agagcaccgc ctcacggtgc atggcattgc caacacagcc tttcaggaca acaaccgtta
     1201 tctgcagaat taccgaattc cgaaggatgg caatctccat gtcgtgctct ttcatggctc
     1261 ggacattcag tccatgccac cacagtatga gcaggatgcc tggttccctt ttgacgagaa
     1321 ggatcttctg gagtgtggtg cccactatgt agcgcttggg cactaccaca gttttcgctg
     1381 tattcccagc gatgaacaca atcccaaagg ctgctatccg ggctgtcctg agtgcctgga
     1441 tcgctctgac agcgacgata aaatcgccct tctcgttgag gtcagtgagg cgggaaaccg
     1501 gatcgagaaa gtgctccttg ctgagtacgg caatgaccaa gtcacactcg actgcaaggg
     1561 ggtgagcaca cgagaggaga tcgagcaaaa gattcaggca ctggccaagg cagggaactg
     1621 gtcgaagaag accgtgcagg taattcttcg aggggagatt gactcggggc tggatttgcg
     1681 tgtagacgaa atacgggagg cggtcaggga tgtggcattt ttccttcaga tacaggatca
     1741 gacggagttt ccctatgatc ttggacaact ggcaagccgc cgtgacgctc ttggagagtt
     1801 cgtgcgaagg atgaaagtcc aagttgaaca aggcggtctg gcggaagagg acatgcgaag
     1861 aactcgtctg gctttgcttc tgggaatcga cgccttcatg cgcaatgagg tgcgaaaacc
     1921 atgatatttg aagacttgct tattcgtggt tttgggaatc tctcgaatgt gaagtttgag
     1981 ttcggacagg gtctcaatgt cttcgtcgct ccgaacgaag cgggcaaatc aacgcttgcc
     2041 gagtgtctgt tccggtgtct ctacggtttc ccaccgttgc gcagcgacga agagagggct
     2101 gcctacaaaa ggtttcagcc actcaatggc gaccgttatt cagcgaaact cagaatcgca
     2161 acagatgagc agacgcacta tgaactggat cgcgattttg ctgcacggac acttcgcatc
     2221 cgaaatgcgg tgacgggtga agacgtcaca gggcagttcc ccgttgaaaa aaaggggcag
     2281 gacgtgctcc tcgctcagaa tctcttgggc attagtcgcg acactttccg aaccatcgca
     2341 tttcttgctc aagggcttgt ggatcgtctt gagaacggcc aggaactcag tgacgagatt
     2401 cgcaagattg ccgattcggg agaattccag acgggctaca agaaggcgct ggagaagctg
     2461 aaaaaggcct ctgatacgat cggcaaaggg gaacgcgctt cctcaaagcc cctttgggaa
     2521 gcacaggaaa gagtcaatac gctgcagcgc gaacgagagg aaatctgcgc caagcgaaaa
     2581 gagcgcatca ccgcattgga acgtatcgag attctgaaac accagttggc agaagccgat
     2641 cgggcttgtc tggaactcga ggtcagaggg ctggcacttc aacaggcgcg ggttcgtgcc
     2701 accatcggcc agttggagac gcttggaagg gagatcgacc aggccagggc aatcgcccag
     2761 cagttggacg gctatcgaaa cgttccctgt caccgtcggg atgagctttt tgtcaacgct
     2821 gacagactca agggtgtcca acaacgcctt gcacagtatc agagccaact tgcctcgctg
     2881 gacgctgaac tgtcagccat caatgatcga ttgtctgaga ccgagcacct cgccaaattg
     2941 tcagagaaag acatcgagca gatggatgtt ctggaaaatg ccctcaaacg tgcggagacg
     3001 gagttggatt tgctggaggg caagatttcc cagagtagac tgacgcagaa gcgtctcgaa
     3061 gatcagatca acgctctcag tcagcgactc aaaggattca caggggagtt gggaaaggat
     3121 ccactggcgt tcgaacaaca acttgcccgc tatgatcagg cttcctcact ggagcgtgag
     3181 gtcgcgttgc gccgcgagca ggtcagcaag acgcaggcgc gcatcgccgg aaagaagggg
     3241 ggctttcgtt ggattgccct gggcgcgggt ctctttctgt tcatcatcgt cgcggcgctg
     3301 aaggctgcgc ctgggggaga attcgtgcct gccatcattt ggggtgttac cgcaggtgtg
     3361 gccggctttc ttttctcgaa tttgggattt cgtctggtca tgaaagggga cgccgagcag
     3421 ctgattctgg agcaggaaac gcttcggact gcggaggtgg aactggcgaa ggttgaaaaa
     3481 gaacgacagc agcagttgtc gaatgcttcg gtgtcaaatc tcaaggaact gcgggagttg
     3541 ttccaactct accgtagcca ccagtcagag ttggaaaacc atcagacgct tcttcaacac
     3601 ctgctcaatg agtctggccg acacgaacag gaactcacca gcgcacaaag tcagattcag
     3661 gactatttga tgcaatgcaa gatcgtggca aaggacgaac cgtttcagga caagtccctt
     3721 ctccgactgc gtcagcagta tctggcgaga aaagagaact gccagaagcg cgacaatc
//
LOCUS       DAOQUC010000206         3765 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_27570_length_3765_cov_3.293531, whole genome shotgun sequence.
ACCESSION   DAOQUC010000206 DAOQUC010000000
VERSION     DAOQUC010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3765
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27570_length_3765_cov_3.293531"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1839
                     /locus_tag="PKH07_04820"
     CDS             <1..1839
                     /locus_tag="PKH07_04820"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HNT34303.1"
                     /translation="LLDVEFDPSLIQHMLPNDDLDLQVLWLSVVEAKGVRFTSEDLAE
                     AFLNRCPYAPGEYAYFKKNYARGIRPPLCGSFNNPYYIQGMGCPIRSEIWSCLAPANP
                     QLAAECAAKDGILDHAGDSVYAEQFLAAMESVAFVGEDLDILIGIGLNLIPPESRISE
                     LIRDVRHWCTYHTDWKQIRGRILQKYGHPDCTNLYQNMGFLLMALILGEKDFAKTLLM
                     AINCGFDTDCTASTAGALLGILSGAESLLKEYPIEDPRFVLGVQAERCSDRILDLAED
                     TARMGLHFAKYLNAHVEFENAPEAPMIELPAPTPISISVNYQGPPAIGLGGSRRLELV
                     FGNSAQWPVEGTARLVLPEGWLADTRKRNLHLIPGSAVSWEVDVAAPGNLDSLNETNI
                     LRVEWQPLNEEPVSHQFGLVGAAVWEVFGPFWQNNVEIPSLKKGESYYAHISGTDNND
                     RADQVRTYHLNTTVDLDRDYMSLEDLLTHGIAGDPATEPRVYNAFEDKISVSNLVGFQ
                     GPCVVYLVRRMFSPHARSLGVQIGHTDAYRLWINERLVSKSDSVDWWTPENVHLLDFH
                     IRKGLNTIVWKLIRRTGEAYFSLNFTKGGPCTDHYCDFASGNPGIH"
     gene            1870..2655
                     /locus_tag="PKH07_04825"
     CDS             1870..2655
                     /locus_tag="PKH07_04825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34304.1"
                     /translation="MRIDCDLHIHSYLSACSNEKDTRTPGAIVSLAEEMGLRAMGFAD
                     HLWTNPNLAPSEWYRPQDESQISRIRNELEGICSKVRVLVGCEAETIAPGKFGITRAF
                     ADQLDFVLLACSHFHQKEFVEQPDGDSPQELGKHIMKFFRSAVSSGLATSIPHPLIPC
                     GSLDKFDEAIASLSDAELQDAFGLAQSHRVALEITVGFIPNEKRPFSIETPIRFLSLA
                     KQAGCKFTLGTDAHSFKQQKRLQELRPLVEALQLTDEDEWCAV"
     gene            complement(2684..3382)
                     /locus_tag="PKH07_04830"
     CDS             complement(2684..3382)
                     /locus_tag="PKH07_04830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34305.1"
                     /translation="MKRLLELRLTAIWILVALSLTGCVRGAKKVPVLPHEDLFTKHVL
                     NVVESYPTDGTHKYWWPKDEVWAGVTRDIRYKREVVAKGDPQGRCYCCGLTWEVFMRA
                     VEQYNKRHWRNVLDHWTREEVDDFRKKWFGSDGNKECLRNAILSYKIGREITNKDEAR
                     RGDFVQLWRANGSGHSVVFDSWIRSETGEIGGMKYWSTQPASSGIGYATETTKGPKAL
                     LLDQTYVMRIGKRR"
     gene            complement(3552..>3765)
                     /locus_tag="PKH07_04835"
     CDS             complement(3552..>3765)
                     /locus_tag="PKH07_04835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34306.1"
                     /translation="NKISAFGGDYCFVDGVYGHQFLARQNVAAALAQKVTDGAMSLAR
                     AKEISHWLFVDNPMRLFGLDRFLGNR"
BASE COUNT          857 a         1039 c         1000 g          869 t
ORIGIN      
        1 ctcctggatg tggagttcga tccctcattg atccagcaca tgctgcccaa tgacgatctc
       61 gatctgcagg tgctctggct ctccgtggtc gaggccaaag gggttcgttt cacgtctgag
      121 gatctggcgg aagcgtttct caacagatgt ccctatgcgc ccggggaata tgcttacttc
      181 aagaagaatt acgctcgcgg cattcgccct cccctttgtg ggtcattcaa caacccctat
      241 tacattcagg gaatggggtg tccgattcgg tcagaaatct ggtcatgcct tgcgcccgca
      301 aatccccagt tggcggcgga atgcgccgcc aaggacggaa ttctggatca cgctggcgat
      361 tcggtctacg ccgagcagtt tcttgctgcc atggaatcgg tggcttttgt cggagaggat
      421 ttggacatct tgattggaat cgggctgaac ctcatccctc ccgagtctcg aatctctgaa
      481 ctgatccgag acgttcgcca ttggtgtaca taccacaccg attggaagca gattcgcggg
      541 agaattctcc aaaagtacgg ccatcccgac tgcacgaatc tataccagaa catggggttt
      601 ctactcatgg cgctgattct gggcgaaaag gacttcgcca aaaccctcct catggcgatc
      661 aactgtgggt ttgacacaga ctgcacggcg agcaccgctg gagccttgct ggggattcta
      721 agcggagccg agtccttgct aaaggaatat cccatcgaag atccaaggtt cgttctgggt
      781 gttcaggcag agcgttgctc tgaccggatt ctggacttgg ctgaagacac cgcccgaatg
      841 ggtctgcatt tcgccaagta tctcaacgct catgtcgagt tcgaaaatgc tccagaagca
      901 ccgatgatag aactgcccgc gccaacgccg atcagcatct ctgtcaacta ccaagggcct
      961 ccagctatcg gtcttggagg atcgaggcga ctggaactcg tctttggcaa cagcgcgcaa
     1021 tggcctgttg aaggtaccgc aaggctggtg ctgcctgagg gttggcttgc agacacgcgc
     1081 aagaggaacc tgcacctgat ccccggttcg gcagtctcct gggaagtgga cgtggccgct
     1141 cccggaaatc tggacagcct gaacgagacc aacatcctca gagtcgagtg gcaaccgttg
     1201 aacgaagaac cagtgtccca tcagtttgga cttgtcggcg ctgccgtttg ggaagtcttc
     1261 ggcccgttct ggcagaacaa tgtggagatt ccttcgttga agaaaggcga gagctactac
     1321 gcgcacattt ccggcacaga caacaatgat cgagctgacc aggttcgaac ttaccatctc
     1381 aacacaacgg tagatctgga tcgagactac atgtcgctcg aagatctact gactcatggc
     1441 attgcgggtg atccggccac agagccacgg gtctacaatg catttgagga taagatctct
     1501 gtgagtaatc ttgtgggatt ccaggggccg tgtgttgtct acctggttcg gcgaatgttc
     1561 tccccacatg caagatcgct tggtgtccag atcggtcaca ccgacgcata tcgtctctgg
     1621 ataaacgaga gactggtgag caagagcgac tcggtagact ggtggacgcc ggagaacgtg
     1681 catctgctgg atttccacat tcgcaaggga ctcaacacga tcgtctggaa actgatccga
     1741 cgcaccggtg aagcctattt cagcctgaac ttcaccaagg gcggcccctg cacagatcac
     1801 tactgcgatt tcgcttccgg gaatccgggg attcactgag atgcgtttgg gattggagca
     1861 tgccacgcga tgagaattga ctgcgatctg cacatccata gttacctctc cgcctgtagc
     1921 aatgaaaaag atacccgcac cccaggagca attgtctcac tggcggagga gatgggtctt
     1981 cgagccatgg ggtttgctga ccatctgtgg acaaatccga atctggctcc cagcgaatgg
     2041 tatcgccccc aggatgaaag tcagatatca aggattcgaa atgaattgga gggcatctgc
     2101 agcaaagtgc gcgttcttgt cggctgcgag gcggagacaa ttgcacctgg aaagtttggc
     2161 attacgcgtg cgtttgccga ccaactggat ttcgtgcttc tggcgtgcag ccattttcac
     2221 cagaaagaat tcgtcgagca accggatggc gattctccgc aggagttggg aaagcacatc
     2281 atgaagttct ttcgctccgc tgtctcaagc ggacttgcta catccattcc tcatccgctg
     2341 atcccttgcg gaagtctcga caagttcgac gaggcaatcg cctcactgtc agacgcggag
     2401 cttcaggacg ccttcgggct cgcacaaagc cacagggttg ccctcgagat aactgttggc
     2461 ttcatcccca acgagaagag gcctttctcc atcgaaacac ccattcggtt tctgtccctc
     2521 gccaagcaag caggatgcaa attcaccctc ggaaccgatg cccacagttt caaacagcag
     2581 aagaggcttc aggaactccg cccgctcgtg gaggcactcc aactcacgga tgaggatgag
     2641 tggtgtgcgg tgtaaagaca ttcagaagcc ttccgccaga tgtctatctg cgcttcccga
     2701 tccgcatcac gtaggtctga tcgagaagca gggctttggg ccctttggtt gtctctgtgg
     2761 catatccgat gcccgaggat gcaggttggg tagaccagta tttcatgccc ccaatttcgc
     2821 cggtctcact gcgaatccag gaatcaaaga ccactgaatg tcccgaaccg ttggcgcgcc
     2881 agagttgaac gaagtctcca cggcgcgctt cgtccttatt ggtgatctct ctgccgatct
     2941 tgtaactcag gatggcattg cgcagacatt ccttgttccc gtcggagccg aaccacttct
     3001 ttcggaaatc atccacctct tcgcgagtcc aatgatccag aacattgcgc caatggcgct
     3061 tgttgtactg ctcgactgca cgcatgaaca cttcccaggt caaaccacag cagtagcagc
     3121 gtccctgagg gtcgcccttg gcgacgactt cgcgtttgta acgaatgtca cgtgtcactc
     3181 ccgcccagac ttcatcttta ggccaccagt acttgtgagt tccatcggtt gggtaggact
     3241 caacgacgtt caaaacgtgt ttggtgaata aatcctcgtg tggcaagacc ggcactttct
     3301 tcgctccccg aacacaaccg gtgagactca acgccaccag aatccagatc gccgtcagtc
     3361 gcagttccaa caatcttttc atcgtctatc gctccttcta tgactatcac ctcgtagtat
     3421 agtctatatg tgcagggaaa gtaagacctg tgtgttgcat cagagaatgc tcgtatcctg
     3481 atggtgtttg aacggtgaag acagaagttt gactgcgacc caaactgacc aactcatcgg
     3541 acggccaagc gtcatcggtt gcccagaaaa cgatcgagtc caaaaagcct catgggatta
     3601 tccacaaaaa gccagtgact gatttctttg gcacgggcaa ggctcatggc tccatcggtg
     3661 accttctgcg caagggctgc tgcaacattc tggcgagcca ggaactggtg tccgtagacg
     3721 ccgtctacga agcagtagtc ccctccgaat gcgctgatct tgttt
//
LOCUS       DAOQUC010000207         3753 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_27680_length_3753_cov_3.139264, whole genome shotgun sequence.
ACCESSION   DAOQUC010000207 DAOQUC010000000
VERSION     DAOQUC010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3753
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27680_length_3753_cov_3.139264"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>3753
                     /locus_tag="PKH07_04840"
     CDS             <1..>3753
                     /locus_tag="PKH07_04840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34307.1"
                     /translation="LYRDTNTFGADGVLLDGSPEANQLWDGTPFTSAAIPVADFIGNT
                     TREKDTFVWDLRNIPSGDYYVYAKMTLGDQEKVIYNCNKVSVAKLPKFWFNHFCQLGD
                     IYLALGEQREIGYYAEDRDSEDKINFFLDTDQNQANGVSFLIQRDVLEADAWGSLMLD
                     GSKLPQGTYYILANVYEPGKSQPSLSVYSDFRVIIQPSEVPRIEVLTPPYTDQPPVVW
                     GDVYDIVWAATDTDDNALIKLYYNSTDFNPATALPIQGINIAPDSNIRYPDALREDDG
                     RGLDGIVPVEWRTWHWDISDVTPGEYYIYATIQDGTQMRSDFSNGAIIINKRPTVRMV
                     KPSSDTSVRQGASYTLWWEAYDKDSQATINIYLDDDMDPSSFGIRVAENLTEIDGFDF
                     HVLDTSVLDIGTYYPVAELSDGVNDPVYSYAPARLIVTLNPPPDIDILRPNLDDTLQV
                     YSRNNFTIEWTDNDDDDAMINLYYDQLGYGFAGTRITQTSISAKTGPQTYAWDVRNLP
                     AGKYYVYGVIVDSVTTVRRYSPGYLMINKMPSLTFIEPDGRNDQVIKGRDYTMSWAAY
                     DPDSTAKIDLFWASGEISPVTPQFSDKVAVGLGIDEKDGVDTLAVNTAAFDATSYYPI
                     ARISDSANPDVWVVSANPVTVLENLAPSIDLLTPATGTMLIKDPSYAVTWNDQDDHNE
                     AIIRLYYDNDQLGTTGVPLPGYYQTVDGKKFDSQNIPQSDAGNLFVWDLTTMPSGVWF
                     VLAVISDGVNPAYATHSIGAIRVNRSPEFQFMKPNTMTERVVQNHVYKFEWIINDPDD
                     NANVTVYLDRNTNREDGGYMLIGGPFDENIKSLDFKPDTMTPPAVYWPLARVTDDVNV
                     EQWIYCPYPIEVLRNVPPTFDFLAPSEGEEVFAANRYYIRWQDADPDDDATIALYYDN
                     DNYGSNGTLIQSGLTENDPGNFFLWNTAERQSGIYYLYAVVDDGFNPPVTKYSPGYLR
                     KVNPIAVDLWLGLLAMDTAGVVYSVTPATGFQTPDLGAGSAVSFKVDSTGSYAYLLLT
                     DGSVVKYDATQELSVLDTGIANPVDMELDVDNQTIHVLSATGEVRSFGPAPALASPYG
                     FPLAEPRLVDLALHPSGLGAYVLEDTGVVHVASVAGATTTIPYYAGPQLGDDRSAVAI
                     RTVSNGAYVVDRKGNLYTMGEAKIFTATPDLTDINPVVDMLVMEELGGYLLRADGQLN
                     IFGDKMLTPGSVGAPSMKSLAWGRRYAEVSQEELIRWLVELFAQRY"
BASE COUNT          849 a         1007 c         1034 g          863 t
ORIGIN      
        1 attgtatcga gataccaaca cgttcggtgc agacggcgtc cttcttgacg gcagtccgga
       61 agccaaccag ctctgggatg gaactccgtt cacatctgct gcgattcctg tggctgattt
      121 catcggcaac acaacccgtg agaaggatac cttcgtctgg gatctccgca acattccgtc
      181 gggagactac tatgtctacg ccaagatgac tctgggcgat caggagaaag tgatctacaa
      241 ctgcaacaag gtttctgtcg caaagctgcc gaaattctgg ttcaaccact tctgtcagtt
      301 gggtgatatc tatctggcac tgggtgaaca gcgggagatc ggctactatg cggaagaccg
      361 ggattcagaa gacaagatca acttcttcct ggataccgat cagaatcagg ccaacggggt
      421 cagcttcctc atccagagag atgttctcga ggccgatgcc tggggcagtc tgatgctgga
      481 tggctcgaaa ctgccgcagg gaacttacta catcctggcg aacgtctatg agcccgggaa
      541 atcacagccc agtctgtcgg tttactcaga cttccgagtc atcattcagc cgtctgaagt
      601 tccgagaatt gaagtcttga ctccgcccta cacagaccag ccacctgtgg tctggggtga
      661 tgtgtatgac atcgtctggg cggcaacgga cactgacgac aatgcgctga tcaagttgta
      721 ctacaactcg accgacttca acccggcaac cgcgcttccg attcagggca tcaacatcgc
      781 tcctgactcg aacatccgct atcccgatgc acttcgcgag gatgacggtc gcggtctcga
      841 cggaattgtg cctgtcgagt ggcgtacctg gcattgggac atcagcgacg tgacgccggg
      901 tgagtactac atctatgcca cgatccagga tggcacgcag atgaggtcgg acttcagcaa
      961 tggtgcgatc atcatcaaca agcgtccaac ggttcgtatg gtcaagccaa gttcagacac
     1021 ttccgtccgt caaggtgcca gctataccct gtggtgggaa gcgtatgaca aggactcaca
     1081 ggcgacgatc aatatctatc tcgacgacga catggatccg tcctcgttcg gcataagggt
     1141 cgccgaaaat ctgacggaaa tagacggatt cgacttccat gttctggaca catctgtgtt
     1201 ggacattgga acctactatc ctgttgcaga actcagtgat ggagtcaatg atccggtgta
     1261 ttcttacgcg ccggcgagac ttatcgtcac tctgaacccg ccaccggata ttgacatcct
     1321 gcgtccgaat ctcgatgaca cgttgcaggt ctactcacgc aacaacttca ctatcgagtg
     1381 gacagacaac gatgacgacg atgcgatgat caatctttac tacgatcaac tggggtacgg
     1441 atttgctgga acccgcatca cgcagaccag catctctgcc aagacaggtc ctcaaacata
     1501 cgcctgggac gtgaggaatc tgccagcggg caagtactat gtctatggag tcattgttga
     1561 cagcgtgact acggttcgcc ggtactcgcc tggatacctc atgatcaaca agatgccgtc
     1621 gctcaccttc attgagccgg acggtcgaaa tgaccaggtg atcaagggac gggattacac
     1681 catgtcctgg gcagcctatg atcccgacag tacggcaaag atcgaccttt tctgggcatc
     1741 tggtgagatt tcaccagtga cgcctcagtt cagcgacaag gttgccgttg gtcttggaat
     1801 tgatgagaaa gatggtgtgg ataccttggc ggtcaatacg gccgcgtttg atgccacatc
     1861 atactatccc attgcgagaa tcagcgactc cgcgaatccc gatgtgtggg ttgtcagtgc
     1921 caatccggtt acggtgctgg agaacctcgc gccgtccatt gacctgctga ctcccgcgac
     1981 gggaaccatg ctgatcaagg atccttccta cgcggtcacc tggaacgatc aggatgatca
     2041 caatgaggcg atcattcgtc tgtactatga taacgatcag ctgggcacga cgggagtccc
     2101 gctgccgggt tactatcaga ctgttgacgg gaagaagttc gattcccaga acattccgca
     2161 gagtgacgcg ggcaacctct ttgtctggga tctgacgacg atgcccagcg gtgtctggtt
     2221 tgtgctggct gtgatttcag acggtgttaa ccccgcctat gcgacgcaca gtattggtgc
     2281 gattcgagtc aaccgttctc ccgagttcca gttcatgaag cccaacacga tgaccgagcg
     2341 tgttgttcag aaccacgtct acaagtttga atggatcatc aatgatccgg atgacaacgc
     2401 caatgtcacg gtctatctgg atcgcaacac caaccgtgaa gatggtggct acatgcttat
     2461 cggtggacca ttcgacgaga acatcaagtc gttggacttc aagcccgata ccatgacgcc
     2521 accggctgtc tactggcctc tggcgagggt cacggatgat gtgaatgtgg aacagtggat
     2581 ttactgtccg tatcccatcg aggtgctcag gaatgttccg ccgacgtttg acttcctggc
     2641 tccctctgag ggtgaggaag tctttgctgc caaccgctac tacatccgat ggcaggatgc
     2701 cgatccggat gatgatgcga ccattgcgct gtactacgac aatgacaact atggcagcaa
     2761 tggtacgttg atccagtcag gtctgactga gaatgacccg gggaacttct tcctgtggaa
     2821 cacggcagaa cgacagtcgg gcatctacta cctgtatgcg gtcgtggatg atggcttcaa
     2881 cccgccagtc accaagtaca gtccaggata tctccgcaag gtcaatccga ttgccgtcga
     2941 tctctggctg ggcttgctgg cgatggatac ggccggcgtg gtctattcgg tgactccggc
     3001 aacaggtttc cagacaccgg atctgggtgc tggcagtgcc gtctccttca aggtggacag
     3061 cacgggcagt tacgcctatc tgcttctcac ggacggttcc gtggtcaagt acgacgccac
     3121 gcaggaactc tctgttctcg acacgggcat tgccaaccca gtggatatgg aactggatgt
     3181 ggacaatcag actatccacg ttctcagcgc tacaggagag gttcgcagct tcggtcccgc
     3241 accggccttg gcctcccctt acggatttcc gctggcggaa cccagactgg tggatctggc
     3301 gctgcatccc agcgggttgg gtgcttatgt ccttgaagac acgggtgttg tgcatgttgc
     3361 ctctgttgcc ggtgcaacga ccacgattcc ctactatgcc ggacctcaac tgggtgacga
     3421 tcgttcggcg gttgccatcc gtaccgtcag caatggtgct tacgttgtgg atcgaaaagg
     3481 caacctgtac acaatgggtg aagcgaagat cttcactgcg acgcctgact tgaccgatat
     3541 caatccggtc gtggatatgt tggtcatgga ggaattgggt ggctatctgc ttcgtgcaga
     3601 cggacaactc aacatcttcg gggacaagat gctgactcct ggctcagtgg gtgctccgtc
     3661 gatgaaatcg ctcgcctggg gtcgccgata tgcggaagtc agccaggaag agctgatccg
     3721 gtggttagtg gaactgttcg cccagagata tca
//
LOCUS       DAOQUC010000208         3738 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_27844_length_3738_cov_3.717350, whole genome shotgun sequence.
ACCESSION   DAOQUC010000208 DAOQUC010000000
VERSION     DAOQUC010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3738
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27844_length_3738_cov_3.717350"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..457)
                     /locus_tag="PKH07_04845"
     CDS             complement(<1..457)
                     /locus_tag="PKH07_04845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HNT34308.1"
                     /translation="MEQDQFIEQVVELADHQETLAVCGHHDEHLRYLSGALGARIVPR
                     GNILKIIGSPEPVGKTRTVLEEMLTTLRRRGSLKRRDIKQLVMLLSQESPEAFEGLWQ
                     GAVRVPSRKKIIKPMSPRQADYLKAIQETDVVFGIGPAGTGKTYLAMAMA"
     gene            complement(669..2225)
                     /gene="dacB"
                     /locus_tag="PKH07_04850"
     CDS             complement(669..2225)
                     /gene="dacB"
                     /locus_tag="PKH07_04850"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HNT34309.1"
                     /translation="MSREKTTVSLIALTVVFLAARSEAAPRNGSDGSGQQSTSQQSMV
                     PEATGEQITLLRQKLDGIFSRSDFRGATWGVYVYSASREEEIYSFNKHTLMVPASNMK
                     LYTTAAALVKLGPNFRYETSLFRTGDIDPSGILNGSLVVRGSGDFSLSARFHNGNALK
                     PFDDWIEELKKAGINKVRGDIVGDDSVFDNKNHGPGWTMDYLDDWYAAEVSGLALNDN
                     CVDVYAYPGSSAGSPAVLKPVPDCSFVKFKNQALTGRSGETSGIRFDRPFRSNEITIT
                     GRVALGTKPQVIWITVSDPTTFFVTVLRERLVERGISVEGGAYSLRDYADKSRVARRE
                     LIAGYYSPPLSELVKVIGRNSQNLYAEQLFKTMGSRLKRNGSFAGGAKVVEEFLSGIG
                     LDSRMYQFFDGSGLSRLNLVNAFGTVRLLDCMRGEQTFPVFLDSLPEAGRTGTLSSRM
                     RGTGAEGNLYAKTGTIRNTRALSGYLTTREGELLVFSMFVNNFTPYSSGKPRMAIDEA
                     CVLLADFARN"
     gene            complement(2546..>3738)
                     /locus_tag="PKH07_04855"
     CDS             complement(2546..>3738)
                     /locus_tag="PKH07_04855"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNT34310.1"
                     /translation="ISSSPCLENRLVYPCRHSKEREDRWEMKMDLAQLHRQVCFGQSE
                     GRIIWQPRIGCWYTDKHFAGEPFPAPFTGMNLYEIYRELGCSARLYEFNSCFKRIEDP
                     APKVEHLQLNETDTQITIATPVGKQTIVVRKTSSSPHPITLKREVATEDELKVATWRE
                     EHSTWQWDQQQYERLLASVGDLGAPTMFMPRMNVQSLYVEKMGVAASVYAIYDWQDSV
                     EAFFRAVDECHIRLIDVINASPIEIINLGENVHAGTLSPDLFLKYHLPACQMRCERLH
                     QAGKFVSSHWDGDTRPLLPYAKETGLDAIEAITPQPQGDVTLEEVKEALGDDIFLLDG
                     IPAVYFDKTFSEETLIECTNRIIELFAPKLVLGISDEISSTGDLERVRLVDRMVNEYN
                     ARFQ"
BASE COUNT          828 a         1011 c          982 g          917 t
ORIGIN      
        1 cagccatggc catcgcgagg taggtctttc ccgttcctgc aggacctatc ccgaaaacca
       61 catcggtttc ctgaatggct ttcagatagt cggcctgccg tggactcatc ggcttgatga
      121 tcttcttgcg cgatggaacg cgaacagcac cctgccacag accctcgaaa gcttccggag
      181 actcctggct cagaagcatg accagttgtt tgatatcccg acgcttcaga ctgcctcttc
      241 ggcgaagcgt ggtgagcatc tcttccagga cggttctggt ttttccgact ggttcaggag
      301 agccaatgat cttgaggata ttcccgcgtg gcacaattcg agcgcccaac gcacccgaaa
      361 gatacctgag atgctcgtca tgatggccac agactgccag ggtttcctgg tgatctgcca
      421 actcgacaac ctgttcaatg aactggtctt gctccaaggc catcaaatcc tctctcgaat
      481 gctgaattga gacgttgaaa gggtgggaca accggtgaag agagaacaga atgctctgtc
      541 tgctcgagcc gactttgtgc aatgacaccg gttccgggga ctcggtggta atgggtcgcc
      601 atttccgggc agttctatat ccatcttcaa catcttgagt gaaatagtaa aggcaatccc
      661 atctgctgtc aatttctggc gaaatccgcc agcagaacac acgcttcgtc aatggccatt
      721 cgcggtttgc ccgaactgta aggagtgaag ttattgacga acattgagaa gaccaacaac
      781 tcgccttccc gtgttgtcag atagcctgaa agcgcacggg tattgcgtat ggttcctgtc
      841 ttggcgtaga gattcccctc tgcgcctgtt cccctcatcc tgctggagag cgttcctgtg
      901 cgtcccgctt cgggaagact gtccagaaag acgggaaagg tctgctcgcc tctcatgcag
      961 tccagaagtc gaacggttcc aaaggcattg accagattca gacgcgacag acccgagccg
     1021 tcgaagaact ggtacatcct ggaatccaaa ccgattccgg acagaaactc ttcaacgact
     1081 ttggctcccc ccgcgaacga accgtttcgc ttcagtcttg aacccatggt cttgaaaagc
     1141 tgttccgcat agaggttctg gctgtttctg ccgatgactt tgaccaattc cgacaacggg
     1201 ggggaatagt aacctgcgat caactctctg cgcgcaactc tggatttgtc ggcgtagtcc
     1261 ctgagagaat acgcgcctcc ttcgaccgag atacctctct cgacgagcct ttcgcgcaaa
     1321 actgttacga agaatgtggt ggggtcgctg acggtgatcc agatcacctg aggcttcgtt
     1381 cccaaagcca ctctgccggt gatggtgatc tcgttgctcc gaaaaggacg atcgaagcgg
     1441 ataccggaag tctcccccga tcggccggtc aaggcctggt tcttgaactt gacaaaagaa
     1501 cagtcgggaa ccggtttgag aacggcgggt gaacccgcac tgctgcccgg ataggcatag
     1561 acatccacgc agttgtcatt gagtgcaaga cctgagactt cggcagcgta ccagtcatca
     1621 agatagtcca tcgtccagcc gggaccatgg ttcttgttgt cgaaaacact gtcgtcaccg
     1681 acaatatctc cccgaacctt gttaatcccc gcttttttca gttcctcaat ccagtcatcg
     1741 aagggcttca gcgcattacc gttatggaat cgtgctgaga gactgaagtc gccagatccc
     1801 ctgacgacca gagaaccgtt caagatgcca ctgggatcaa tgtcacctgt tcgaaaaagc
     1861 gatgtctcgt atcggaaatt gggtcccaac ttcaccagcg ctgccgcagt tgtgtagagt
     1921 ttcatgtttg aggcaggcac catcaatgtg tgtttgttga aagaatagat ctcctcttct
     1981 cttgaagcgg agtagacata gacgccccaa gtcgcgccgc gaaaatcgga tctgctgaag
     2041 atgccatcaa gtttttgccg aagcaaggtg atctgctcac ccgtggcttc aggaaccata
     2101 gactgttgag atgtgctttg ctgaccggaa ccgtcactac catttctcgg agcggcctcg
     2161 ctgcgggcag cgaggaacac caccgtcagt gctatgaggg aaactgttgt cttttcgcga
     2221 ctcatccttt ctcccttcaa tgtctgtgtc gtgtgcagtc attgcaggct tcgctgcttt
     2281 gcgaatcatg aagcccgcag tcttgagtct gttcaaacgg acatatctgc gttccagtcg
     2341 ctcttgctgt tttggcggca cgctgatggc aacgctggtc tcgggcaaac ctcatttgtc
     2401 atctttgatc ttggttggct tacaaaggtg tcccttcttt ttcgagacgg caccgcattt
     2461 ggtgcattta tagtggcctt ttttgggctt gtccttggat tttcctttca aacatcccat
     2521 cggctgactt ccaaagtttg cgcggctact gaaagcgcgc attgtattca ttcaccattc
     2581 ggtcgacaag acgaacccgc tcaagatcgc ccgttgatga aatctcatca gagatgccca
     2641 atacaagttt gggagcgaac aattcgataa tccggttggt gcattcgatg agggtttctt
     2701 cggagaatgt cttgtcaaag tacactgccg gaataccgtc cagcaggaaa atatcgtctc
     2761 cgagcgcttc tttgacctct tcgagcgtga cgtctccctg cggttgcggt gtgatggcct
     2821 cgatggcgtc caggccggtt tccttggcat agggcagaag aggcctggtg tcgccgtccc
     2881 agtgggagga aacgaacttg ccagcctgat gcagacgctc gcaacgcatc tgacacgcgg
     2941 gcaggtgata cttgaggaac aggtcagggg acaaagttcc cgcgtggacg ttttccccaa
     3001 ggttgatgat ctcaatgggc gaggcgttga tgacatcaat gagtcgaata tgacattcgt
     3061 caacggctcg gaagaaggct tcgacgctgt cctgccagtc atagatggca tagaccgagg
     3121 cggcgactcc catcttctcg acgtacaggc tttgaacgtt catccgaggc atgaacatgg
     3181 ttggcgcacc caggtcgccg acacttgcca acagtctttc atactgttgc tgatcccatt
     3241 gccaggtgct gtgctcttca cgccaagtgg ccactttgag ttcatcctcg gtggcgactt
     3301 cgcgcttgag cgtgatggga tggggactgc tggaagtctt tcgaacaact atcgtctgtt
     3361 tgccgaccgg agtggctatg gtgatctgtg tgtcggtctc gttcaactgg agatgctcca
     3421 ccttcggagc cggatcttcg atccttttga aacaggagtt gaactcgtac aaccgcgccg
     3481 aacaacccag ttcacggtag atttcataga ggttcatgcc cgtgaaggga gcagggaacg
     3541 gctcgcccgc gaaatgcttg tccgtgtacc agcagccgat tcgaggctgc cagataatcc
     3601 tgccttccga ctgtccaaaa cacacctggc gatgaagctg tgccaagtcc attttcattt
     3661 cccaacgatc ctctcgctct tttgagtgcc gacatgggta aactagacga ttctccagac
     3721 aaggggaaga ggaaatcg
//
LOCUS       DAOQUC010000209         3735 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_27887_length_3735_cov_3.976902, whole genome shotgun sequence.
ACCESSION   DAOQUC010000209 DAOQUC010000000
VERSION     DAOQUC010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3735
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27887_length_3735_cov_3.976902"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..424
                     /locus_tag="PKH07_04860"
     CDS             <1..424
                     /locus_tag="PKH07_04860"
                     /inference="COORDINATES: protein motif:HMM:NF027666.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4976 domain-containing protein"
                     /protein_id="HNT34311.1"
                     /translation="TLIVSAPSQLAPGSKSDALVEFVDIYPTLCELCGLTVRSELEGL
                     SMAPLLNDPQKTWKSAAFSQYPRPEGIMGYTMRTERYRYTEWIQDKTSLVGTELYDHI
                     LDPDENVNCAAEPDKSELVKQLSAALHAGWKSALPLAQ"
     gene            928..1686
                     /locus_tag="PKH07_04865"
     CDS             928..1686
                     /locus_tag="PKH07_04865"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HNT34312.1"
                     /translation="MPSAKPELLSAEIHPTAVLGRNVQIGVGTSIMAHVVIGDDVCIG
                     AHVTVFPGCYIGEGTVIGERTVLKPCVVVRERTQIGHDVMVDSGAVIGSDGFGFAPNK
                     ETGKIEKIPQVGFVVVKDGAHIGANATLDRATLGKTIVGENTIVGDLAQIAHNAVLGD
                     ETLVEPQVGICGSTCIGSRVEIGRRAGIVGHLKVEDGAKIQAGSGITKDVSSGSVIGG
                     WPSVPLKELEERETLQNQLPELFKRVQSLEKRLS"
     gene            1769..2506
                     /locus_tag="PKH07_04870"
     CDS             1769..2506
                     /locus_tag="PKH07_04870"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNT34313.1"
                     /translation="MIFQSVAEYYEILSDNASRLQREQKLLMRLLAEAPGTRVADVAC
                     GTGLHADFFASLGAEVNGFDISPEMVDVARGTRKTSNVTFQVGDMRTLSGGLWDLILC
                     IGNSLSLLGSLDEIRETFVHVHSCLSPGGLFAIQILNTQSEQASKPRHRVEHKALDDG
                     SIVAVKNLIPHEGGTLLSLTFFHLQGDRMSSVSETARLLHLTLEQISQIAEQSGLKVT
                     NVYGGLDGSPYDPSTSGDLVVVMRGDS"
     gene            2681..>3735
                     /locus_tag="PKH07_04875"
     CDS             2681..>3735
                     /locus_tag="PKH07_04875"
                     /inference="COORDINATES: protein motif:HMM:NF014851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34314.1"
                     /translation="MVRMRVSRDRHRVVKGLCIVALLLFTRGGRAEGTPRAEHPRPDM
                     MREQWLSLNGVWDFAETDDDTDETFLGDISYPDEIIVPFCRESSLSGLGRLGFVRNVW
                     YRRSFALPDNWAGKRVLVHIGACDWKTRVWINGVFLGEHRGGNAPFSFELTKHLGTAE
                     NTIVIHAFDDLRSGMQAGGKQSSREESHGCFYTRTTGIWQTVWLEAVGSSFIRQYIVT
                     PDVANSRVMIQVDVDGSRQGLMIFAEAVVEGKVAAKAATRADGQSNILTLNLGKKQRL
                     WSPEEPFLYDLDLRLLEGKTELDRLRGYFGLRSVSIQGAAILINGRATFQRLVLDQGF
                     YPEGIWTAPSDEALKRD"
BASE COUNT          837 a          904 c         1065 g          929 t
ORIGIN      
        1 cacactgatc gtgtcggcac cttcccagtt ggcacctggc tcaaagtcgg atgccttggt
       61 cgaatttgta gatatctatc caacgttatg tgaactctgc ggtctgacag ttcggtcaga
      121 actggaaggg ttgagtatgg cgccactcct caacgatcct caaaaaacct ggaagtcggc
      181 ggcattcagt cagtacccga gaccagaggg tatcatgggc tataccatgc gcacggaacg
      241 atatcgttac accgaatgga ttcaggacaa gaccagcctc gtcggcactg aactctacga
      301 tcacatcctg gatccggatg agaatgtgaa ctgcgctgcc gaaccggaca agtcggaact
      361 ggtcaagcag ctcagcgcgg cacttcatgc gggttggaaa tcggcgcttc ctctggcaca
      421 gtaaggtgcg cgttttgatg gagtccaccc tcttgttgga ctgtctccca tttcctgatg
      481 gggctgtgtt tgggtgcagt tcgccttggc tttgaaccca aatggggtat aaataccaca
      541 caacgaagat gagaccctga atcggggtat ggatgcccga tttctcagcc cagttttatg
      601 gatacgagcc acccagggca aaaagaatct ctgtttcgcc agacatttca cggcacgctt
      661 gcccgagatg attatcccat aagttgttgt ttctcaatat attaaatcca tgtagatgtt
      721 catcggagtt cggggatatg gctggttggc ctgatatctg cttccaggtc ttgtggaaat
      781 gagaacgagg caagatttgg cacacttcct gcgttcttta gtcgaggttg aacggtctaa
      841 tagaagggaa aagaaaaccc aatggtcacc tgcccgaaat cacagagtga aagaatggga
      901 tggagcacga agaatgcagc ggttttgatg ccttctgcaa aacctgagtt gctctcggca
      961 gaaattcacc caacagccgt tcttggaagg aatgttcaaa ttggcgtggg aacatcgatc
     1021 atggctcatg tcgtcattgg cgatgatgtc tgcatcggtg cccatgttac tgtttttccc
     1081 ggatgctata tcggcgaggg aaccgtcatt ggtgagcgaa ctgtcctgaa accctgtgtc
     1141 gtcgttcgtg agagaaccca gatcggtcat gacgtgatgg ttgattctgg tgcggttatc
     1201 ggaagtgacg gttttggctt tgcccccaat aaagaaaccg gaaagattga gaagattccc
     1261 caagttggtt ttgttgtcgt caaggatggg gctcacatcg gagccaatgc caccctcgac
     1321 cgagcaactc ttggcaagac gattgttggc gagaacacaa ttgttggcga cttggctcag
     1381 atcgcgcata acgccgtttt gggtgatgag actttggtgg agcctcaggt tggcatttgc
     1441 ggaagcacgt gcatcggctc ccgagttgaa atcggacgac gcgcaggcat cgtgggtcat
     1501 ctgaaggttg aggacggcgc caagattcag gccggaagcg ggatcaccaa ggatgtctcc
     1561 tctggttccg tcatcggtgg ctggccttca gttcctctaa aggaactcga agagcgggaa
     1621 actctccaga atcaacttcc ggaactgttc aagcgtgtgc agtctttgga gaagagactc
     1681 tcttagaact gccctttgac aaatcagaaa caataaagat acgctgaatg ggtgaactgt
     1741 tcagcgtttg ttttttggga gaagcacaat gatcttccag tccgttgctg agtactacga
     1801 gattctgtct gacaatgcgt cccgtcttca gagggaacaa aaactgctga tgagactgct
     1861 ggcagaagcg cccgggacac gtgtggcgga tgttgcctgt gggacaggac ttcatgccga
     1921 tttttttgca tccctgggtg ccgaagtgaa tggttttgac atcagtcctg agatggtaga
     1981 cgttgcccga ggaacccgca agacgagcaa cgtcacattc caggttggcg acatgcggac
     2041 actctctggc ggcctgtggg atttgatcct ctgcatcggg aactccctct ctcttcttgg
     2101 gagtctggac gagattcgag aaactttcgt ccacgttcac tcctgcctct ctccaggcgg
     2161 acttttcgcc attcagattc tcaacacgca gtccgaacaa gcatccaaac caagacatcg
     2221 cgtggagcac aaggctctcg atgacggctc cattgttgcc gtcaagaatc tgattcctca
     2281 cgaaggcggg actcttctgt ctctgacgtt ctttcacctg cagggagatc ggatgtcgag
     2341 cgtctcggag accgcacggc tcttgcatct cactctggag cagatttctc agattgctga
     2401 acagagcggt ttgaaagtca caaatgtcta tggcggactt gacggcagtc cttatgatcc
     2461 gtccacttcg ggtgatcttg ttgtggtgat gagaggagac agttgaccca tacactcatc
     2521 gttcgggatc tttccaaacg gactcctcat atcatgaatg catcgtttgt tctgagcagg
     2581 caagcgcctg cgggtgtgtg ggctatgccc gctcccttgc ttgctcagga gatgacacgt
     2641 ttctgtagga cttcgcgtcg ccaagaggaa aggatttcag atggtcagaa tgagagtctc
     2701 gcgagatcgg catcgtgttg tgaagggtct ttgtattgtg gccttgcttc tgttcacccg
     2761 tggtggtcgt gccgagggaa cccctcgtgc ggagcatccg cgccctgaca tgatgcgtga
     2821 gcagtggctg agtttgaatg gggtgtggga tttcgctgag acagacgatg atactgatga
     2881 gacctttctt ggcgacattt cttatccgga tgagatcatc gtaccgttct gtcgcgagag
     2941 ttccctgtcg ggtttggggc gtctgggttt tgttcgcaat gtctggtacc ggcgctcgtt
     3001 cgcgctgccc gataactggg ctggcaaacg cgttctcgtt cacattgggg catgtgactg
     3061 gaagacgaga gtttggataa atggcgtctt tctcggtgag cacaggggag ggaacgctcc
     3121 gttttcgttc gagctgacaa agcacctcgg gactgccgag aacaccattg tgatccatgc
     3181 cttcgacgat cttcgaagcg gaatgcaggc ggggggaaaa cagtcttcgc gcgaggagag
     3241 tcatggctgc ttctacacac gtacaacggg catctggcag accgtctggc tcgaagccgt
     3301 cggttcatca ttcatccggc aatacatcgt gacaccagat gtcgccaact cccgtgtcat
     3361 gatccaggtt gacgtggacg gcagcagaca agggcttatg atttttgccg aagccgtcgt
     3421 agagggcaag gtcgctgcca aggcggcgac acgtgcggat gggcagagca acattctcac
     3481 gctgaatctt ggaaagaaac aacggttgtg gtcaccagaa gaaccgttcc tttacgatct
     3541 ggatcttcgg ctgcttgagg gcaagaccga gttggacaga cttcgaggat attttggttt
     3601 gcgctctgtc tcgatacaag gtgcagcgat tctgatcaac ggccgtgcta cgtttcagcg
     3661 gttggttctg gatcaaggct tctatcctga aggtatttgg acagcaccga gtgatgaggc
     3721 actcaagcgg gacat
//
LOCUS       DAOQUC010000210         3729 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_27968_length_3729_cov_3.215297, whole genome shotgun sequence.
ACCESSION   DAOQUC010000210 DAOQUC010000000
VERSION     DAOQUC010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3729
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27968_length_3729_cov_3.215297"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1157
                     /locus_tag="PKH07_04880"
     CDS             <1..1157
                     /locus_tag="PKH07_04880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34315.1"
                     /translation="LQETPIITQGAFDSQNTAYWDCVQKKYVANIRTFEAGFRTVSRS
                     VSTDFRNWSTPQFWTYGDTPREHLYTNAAVPYYRAPQFWLALPMRWERNRLTIWEDDT
                     PIDGVSDGVFLSSRNGQNWDRRFMEGFIRGGLDPLNWGNSHGNNAPVWGIVPTGDNEM
                     SIYWTEHFGASIPMGAPEEKSQKEGEVTPAEDSGTAPRIRRGVLKPDGFVSIHAGYYP
                     GEFTTKPLVFVGREMIINYETSAVGTIDVEFQDINGNPIPGFTLADCVDTYGDEVERP
                     ILWQRGVDYTTNVSALSGIPVKIRFVMNDADLYSIRFAESETLTPEPTLTPTESPTET
                     PTGMDTETPTETPADSPTETETPTETPTETPVDTPTPTTTENPVTVWQVY"
     gene            1369..2955
                     /locus_tag="PKH07_04885"
     CDS             1369..2955
                     /locus_tag="PKH07_04885"
                     /inference="COORDINATES: protein motif:HMM:NF025681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxGYxYP family putative glycoside hydrolase"
                     /protein_id="HNT34316.1"
                     /translation="MAGGTYDVISLEGLQPEQVIVLASLQGLVNRSAPRAYFLTKPDG
                     PEKHWMEWYSDYALVPNEITVDEFLAKYVGEAKGYIVYDPRLPDTLNIAVSMAGVNEC
                     VICHPSEEKKVAGLGLPCFQNLCGRWSDWKAAYDWAINELMPETNRMTLANYDYGTGV
                     QAKPTFDFLISRKGFCMGVSVNEADFPDEPELWHRVLVDAPEGAMMIGWHTARDTEAT
                     HVAFCSRYDVLVYCAGAWNMSFHQHVAHQYEYSQDHCERAECEPEGRYATMVLSDGDS
                     WHSMVDLQKHFWMHPQRGEVALGWEVSPIFARLAPALLEYYYETRTEKDYLVCGPSGI
                     GYNYLSIFGSWEDFVDKTSDVMSEVSLQAIWVINREVKHLPGGIVEHKLRGRTIHYTK
                     AQLEEFGGLKDQNGADLVDPEMVERYVKGMPMAMGFFQGWERIPGEQPRWLDGKVWCP
                     TAAVIGKNVDASVAEFEQAAQEQQPGPAFVSGHVNCYDATMETVIEVGKALESKGFKV
                     VRADEFLQLAQDAFRKGFRK"
     gene            3040..>3729
                     /locus_tag="PKH07_04890"
     CDS             3040..>3729
                     /locus_tag="PKH07_04890"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNT34317.1"
                     /translation="MKQPNVVFLMVDQHNAKCLGCYGSQHVHTPNLDALASRGVRFTS
                     AYTQCPICSPSRMCYLTGQYAHNHGYFGLRGPRPIQLPNLFSHFKGHGYHTAAIGKVH
                     LPEGWIDPNCDFFCEAVFGRSRSMGESYEEFMRRHGIEDPEDEVTRNRLAVGQFLESI
                     DGRPDWLPKELSREGFSVSAASEFVRNRPKDKPFLLWMSLSKPHPEYKPAQEFWDMYP
                     EIPLPPSADDEP"
BASE COUNT          869 a          950 c         1069 g          841 t
ORIGIN      
        1 aactgcagga gacaccaatc atcacccagg gagctttcga ctcgcaaaat acggcatact
       61 gggattgtgt gcaaaagaag tacgtggcaa acatccgcac ctttgaagcg ggttttcgga
      121 ctgtgtcccg gtctgtttca acagacttcc ggaactggtc aacgccgcag ttctggacgt
      181 acggtgacac cccacgagaa catctgtaca ccaatgcggc tgttccctac tatcgcgctc
      241 cccagttctg gttggcattg cccatgcgtt gggagcggaa ccggctgacc atttgggaag
      301 acgacacgcc gattgatgga gtttctgacg gtgtctttct ctccagccgc aatggccaga
      361 attgggatcg gcggttcatg gaaggcttca ttcgtggcgg cttggatcca ctcaactggg
      421 gcaactccca cggcaataac gctcccgtct ggggtatcgt tcccactggg gacaatgaaa
      481 tgtcgatcta ctggacagag catttcgggg catccatacc catgggggca cctgaggaga
      541 agtcccagaa agaaggagag gtgacgccag cggaagactc cggcaccgct cccagaattc
      601 ggcgcggtgt tctgaagccc gacggcttcg tgtccattca tgcaggttat tacccagggg
      661 aattcaccac caagcctctg gtctttgtcg gccgcgagat gatcatcaac tatgagactt
      721 ctgccgtggg cacgattgac gttgaatttc aggatatcaa cggcaatccc attccgggat
      781 tcaccttggc cgactgcgtg gacacctacg gagacgaagt cgaaagaccg attctctggc
      841 agagaggtgt ggactataca accaacgtca gcgctctttc aggaataccg gtcaagatcc
      901 gatttgtgat gaatgacgcg gatttgtact ccattcgatt tgccgagtcg gagaccctca
      961 cacccgagcc aaccctcacg ccgaccgagt cgccgacgga gacgccaacc gggatggata
     1021 cagagacccc gacagagaca cccgccgact ctcctaccga gacggaaact ccgacagaaa
     1081 ctccaacgga gacaccagtt gatacgccca cacccacaac aacggagaat ccggtcactg
     1141 tctggcaggt ttactgagac tttcgtcctg cataccgact gcatctggtg aagctcgtct
     1201 tttgcaccag gtcttgtcca tgctgcgtga tttgaagcac ttactcaata tcggtagact
     1261 ttgaccctgc atactgctat tctctcccgt tgaaatagcg cccgcctacc aaagcggggg
     1321 tcagtgattt tttttcagga gtcagtagac tgggaggcaa agagagttat ggccggtggt
     1381 acttatgacg tcatttcttt ggaagggttg cagcccgagc aggtcattgt gttggcgagc
     1441 ctgcagggtc tggtgaaccg ttccgctccg agagcatact ttttgacaaa acccgatggc
     1501 cccgagaagc attggatgga gtggtactct gactatgctc ttgtgcccaa cgagatcacg
     1561 gttgatgaat tcctagccaa gtatgtcggg gaagccaaag gctatatcgt ctacgatcca
     1621 agacttccag acactctgaa cattgcggtg tccatggctg gagtgaacga atgtgtaatc
     1681 tgtcatccca gcgaggagaa gaaggtggcg ggtctcgggc tgccctgctt tcagaatctc
     1741 tgcggccgtt ggtcagactg gaaagctgcc tacgattggg caatcaatga gttgatgccg
     1801 gaaaccaacc ggatgacgct tgccaactac gattacggaa cgggcgttca ggcaaaaccg
     1861 acctttgatt tcctgatatc ccgcaaggga ttttgcatgg gtgttagtgt caacgaagcc
     1921 gattttcctg atgagccgga attgtggcac cgagtgctcg tggacgctcc cgagggagcg
     1981 atgatgatcg gctggcacac ggcgcgagac acggaagcaa cgcatgtggc tttttgcagt
     2041 cgctatgacg ttctggtgta ttgtgccggt gcctggaaca tgtcctttca tcaacatgtc
     2101 gcgcatcaat atgagtattc gcaggatcac tgtgagcgag ccgagtgcga gccggaaggg
     2161 cgctacgcga cgatggtatt gtccgatggc gactcctggc attcgatggt ggatcttcag
     2221 aagcacttct ggatgcatcc ccaacgtggt gaggtggcct tggggtggga ggtgtcgccg
     2281 attttcgcgc gactggcacc ggctttgttg gagtactact acgaaactcg aaccgagaaa
     2341 gactatctgg tctgtggtcc ttcgggaatc ggatacaatt acctgagcat ttttggcagt
     2401 tgggaagatt ttgtggacaa gacttccgat gtcatgagtg aagtctcact tcaggcgatc
     2461 tgggtcatca accgagaagt gaagcatctg cctgggggca tcgtagagca caaactgcgt
     2521 ggccgaacaa tccattacac caaggcgcaa ctggaagagt ttggtggact caaggatcag
     2581 aacggtgcgg atcttgtaga tcctgagatg gtggagaggt atgtcaaggg gatgccgatg
     2641 gcgatgggct ttttccaggg ctgggaacga atccctggcg aacagcctcg ttggctggac
     2701 ggaaaagtct ggtgtccgac cgcggcggtt attggcaaaa atgttgacgc aagcgttgcc
     2761 gagttcgagc aggctgctca ggaacagcag ccaggtccgg ctttcgtctc gggtcatgtg
     2821 aactgctatg atgcgacgat ggaaacggtg atcgaggtgg gcaaggctct ggagtcgaaa
     2881 ggcttcaagg tagtccgtgc ggatgagttc ctgcaattgg cgcaggacgc attcaggaag
     2941 ggcttccgga agtaaaccga gcacagagca atggcgcaga aaccacatac acttgcgttt
     3001 tcttttgctg cggagaaacg ctttttggag tggtaaccga tgaagcaacc caacgttgtc
     3061 ttcctgatgg tggatcaaca taacgccaag tgccttgggt gctatggaag ccagcatgtg
     3121 catacgccga acttggatgc tttggcttca agaggagtcc gattcacttc agcatacacg
     3181 caatgtccga tttgttcccc aagtcggatg tgctatctga ccggacagta tgctcacaat
     3241 cacgggtact tcggactcag gggaccgaga cccattcagt tgccgaacct gttttcccac
     3301 ttcaagggcc acggttacca taccgcagca ataggcaagg tacatcttcc ggaaggttgg
     3361 atcgatccca actgtgattt cttctgtgaa gccgtgtttg gacgaagcag gagcatgggt
     3421 gagtcctatg aggagttcat gcgtcggcac ggtatcgaag atccagaaga cgaagtcaca
     3481 cgcaatcgct tggccgtggg acagtttctg gagagcattg atggcagacc cgactggctt
     3541 cccaaagagc tgagccgcga aggcttctct gtctctgctg catccgagtt tgtccggaac
     3601 agacccaagg acaagccttt tctgctgtgg atgtcgttga gcaaaccaca tccggaatac
     3661 aaaccggctc aagagttctg ggatatgtat cccgaaatcc ccctgccccc aagcgcggac
     3721 gatgaacca
//
LOCUS       DAOQUC010000211         3727 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_27981_length_3727_cov_3.955882, whole genome shotgun sequence.
ACCESSION   DAOQUC010000211 DAOQUC010000000
VERSION     DAOQUC010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3727
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27981_length_3727_cov_3.955882"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="PKH07_04895"
     CDS             complement(<1..189)
                     /locus_tag="PKH07_04895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34318.1"
                     /translation="MNTCGRVGYLNLLPGGNTSPNSKRRMSLTAYAREDLTFSLAAPL
                     LFEVPRGLSEASRFLAADI"
     gene            complement(194..>3727)
                     /locus_tag="PKH07_04900"
     CDS             complement(194..>3727)
                     /locus_tag="PKH07_04900"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNT34319.1"
                     /translation="LVAIAESICCKQYDLDAVFEEYVRRASSFSLGSTASLWISETDD
                     RIRCICDISNGGEKRSSKKSSVGTPIDLSAAAGVRSDIAFSDNSHAREMNALRQAGVL
                     LPQTRSCLLVPLKSKGTRTGFALHERFEAPEQWNENGLHFVRSASGFLAYAIEEKRIH
                     ESLEELRRTDKDYRAAIENTSGVPYRLLLPERRYAFIGSGLERLIGISRDEFTLDRLK
                     QSIREVVLMDAEGPHDPDQYKKAFADGKLTQYRADLLVETADGGLKWLSDCSVPVYDS
                     SGTVVGSLGILQDITSRKLAERDLLRKNARLRTLNEIAKLTSGRMLPEELAEKIFNAV
                     HHTIACDAFFLNQYRERDDALRRLKYYDTIDGVIQEVQPRDPAFELKPYTKSCANELR
                     EVLLERRPVMILRSPDELVESGLVVYGSDRRSASLLFVPLVAGDQCVGLMSVQSYQYS
                     VYSQEDLDFLVAVAQQTGPAFEAAILSKEILDREQAQRCFSEKLTALIQVTSELASAE
                     SLDDLCRGAVELGRSRLGFDRVSLWLLTEEPHTVEGTFGTDEFGKTRDERDVRLRLDP
                     NLPSCRVLSGDDSLILKEDWELYNHLHQVVGKGCYAVAAVWDGDRVIGFLSTDNLLTR
                     QNISEYQTEILRLYGSVLGTLYSRKRAEQQLRHSEQIYRTAIEWASGVPYQFRVGDGQ
                     FDFIGSGLESLLGISAEEFTPERFEAMIVEQTVTDPRGPSDPSEYRESFLDGVLTHYR
                     MDVKVRLADGQEKWLSDTQVPYFDTLGRVEGSLGILQDISDRKKAEEDKHRLETRILH
                     AQKLESLGILAGGIAHDFNNLLMGMLGHADLALSQMPRESPARENVTQIETAALRAAD
                     LTRQMLAYSGKGQFLIQPLNLSKLIEEMVHLLEVSISKRVTLRFSFAQNLPSVLGDPG
                     QLRQVFMNLILNASDAIGDHDGVISVTTGIVKADRDYLAQTYFDENLPEGEYVYAEVS
                     DTGCGMDRETIRRIFDPFFTTKFTGRGLGLAAVLGIVRGLKGAIRVYSHPGAGSTFKV
                     LLPSCDARVLAADQEETQLEMWRSDGVILVVDDEESVLTVAQRALELRGFSVLTAKDG
                     LKGVDLFRRHAEEIRLVLLDMTMPLMDGEEAFFEMKKIKADVPVILSSGYTEQEATNR
                     FHSNELSGFIQKPYRPIDLIKKIKALLDQKP"
BASE COUNT          845 a         1049 c          954 g          879 t
ORIGIN      
        1 aatatcagct gccaaaaagc ggcttgcttc cgagagacct ctgggaacct caaagagcaa
       61 gggtgctgcg aggctgaacg tcaaatcttc tcgtgcgtag gctgtgagac tcatcctgcg
      121 tttcgagttg ggagaggtgt ttccgccagg gagaaggttc agatagccca cgcgcccgca
      181 ggtgttcacc tgctcagggt ttctgatcca gaagcgcttt gatcttcttg atcaggtcga
      241 tgggtctgta gggcttttgg ataaaaccgc tcaactcatt gctgtgaaac cgattggtcg
      301 cttcctgttc ggtgtagcca ctggacagga taacgggaac atcggccttg atcttcttca
      361 tctcgaagaa ggcttcctct ccgtccatga gaggcatggt catgtcaagc agaacaagtc
      421 gaatctcctc ggcatggcgt cggaaaagat cgacaccctt gagtccatct ttggcggtca
      481 agacactgaa acccctcagc tcaagggcgc gctgtgcaac ggtcaaaaca gattcctcgt
      541 catccacaac gaggatgact ccatcgcttc tccacatctc caactgcgtc tcttcctgat
      601 cagccgccaa aactcgggca tcacaggatg gcaagagcac tttgaaggta cttcctgccc
      661 ccgggtgact gtaaactcgg atggcacctt tgaggcctcg gacgattccc agaacggctg
      721 ccagtccgag cccgcgtccc gtgaatttgg tggtaaagaa cgggtcgaat atccgcctga
      781 ttgtctctct gtccatgcca caacctgtgt ctgaaacctc ggcatagacg tattcccctt
      841 caggcagatt ctcgtcgaaa taggtctggg cgaggtagtc ccgatcggcc ttgacaatgc
      901 ctgtggtgac ggagatgaca ccgtcgtggt ctccaatcgc atccgaagca ttcaggatca
      961 agttcataaa gacctgccga agttgacctg gatcccccag cacagaaggc aggttctgcg
     1021 caaaactgaa acgcagtgtg accctcttgg aaatgctgac ttccagcaga tgaaccatct
     1081 cctcgatgag tttggagaga ttcagaggct ggatcaggaa ctgccccttg ccggaatatg
     1141 ccaacatctg tcgcgtcaaa tccgcggcgc gcagtgctgc ggtctcgatt tgggtgacgt
     1201 tctcgcgcgc cggagattct cgcggcattt gagaaagcgc caggtctgcg tgacccagca
     1261 ttcccatgag caggttgttg aagtcatgtg cgatgccgcc cgcgagaatt ccaaggctct
     1321 ccagtttctg ggcatgcaga attcttgtct ccagacggtg cttgtcttcc tcagcctttt
     1381 tgcggtcgga gatgtcttga agaattccca aagacccttc cacccttccc aaagtgtcaa
     1441 agtagggaac ctgggtatca gacaaccatt tctcctgacc gtctgccaag cgaaccttca
     1501 catccattct gtagtgtgtg agtacaccat ccagaaaaga ttcgcggtac tcagacggat
     1561 cggacggccc tcttgggtca gtgacagtct gttcaacaat cattgcctca aatcgttcgg
     1621 gagtgaactc ctcggctgag atgcccagca gggattccag acctgaaccg atgaaatcga
     1681 attgaccatc tccaacgcga aactgatagg ggacgccgga agcccattct atcgccgttc
     1741 ggtagatctg ctcagaatgt cgcaactgct gttctgcgcg ctttctggaa tagagggtgc
     1801 ccaagactga accgtaaagc ctcagaatct ctgtctggta ctctgatatg ttttgtctcg
     1861 tcagaagatt gtccgtgctc aaaaagccga tgacgcggtc tccatcccag acagctgcca
     1921 cggcgtaaca gccctttccc accacctgat gcaggtgatt gtagagctcc cagtcttcct
     1981 tgaggatcag tgaatcatca cccgagagaa cgcgacagga aggcaggttc ggatccagtc
     2041 tgagcctcac atccctttcg tcacgcgttt ttccgaactc gtccgttcca aaggtgcctt
     2101 ccaccgtatg gggctcttcg gtcagaagcc acagactgac tcggtcgaac cccaagcggc
     2161 tgcgacccag ttccaccgca cctcggcaga gatcatcaag cgactccgca gatgcaagtt
     2221 cactcgtgac ctggatcagt gcagtcagtt tctcactgaa gcatctctgc gcctgttcgc
     2281 ggtcaagaat ctccttgctc agtatggctg cctcaaacgc agggcctgtc tgctgggcga
     2341 cggcaacaag gaaatccaga tcttcctggc tgtatactga atactggtag ctttgaacag
     2401 acatcaatcc aacgcactgg tctcctgcaa ccagaggcac aaacaacaac gaggcagaac
     2461 gccgatcact gccgtaaaca accaaaccgc tttccaccaa ttcatctgga gatctgagaa
     2521 tcatcacggg tcttctttcc aggagtacct ctctcagttc attggcgcag gacttggtgt
     2581 agggcttgag ttcgaaggcg ggatcccgag gttgaacttc ctgaatcaca ccatcaattg
     2641 tatcgtaata cttcaggcgt cggagtgcgt cgtctcgttc ccgatactgg ttgaggaaga
     2701 acgcatcaca ggcgattgtg tggtggacgg cattgaatat cttttcggcg agttcctcag
     2761 gcaacatcct tcctgaagtc agcttcgcaa tctcattaag tgtcctcagt cgggcattct
     2821 tcctgagtag atcccgctcg gcaagtttgc ggctggtgat gtcctgaagt attcccagcg
     2881 agcccacaac ggttcccgaa ctgtcataga ccggcaccga gcagtcgctg agccacttca
     2941 aaccgccatc ggccgtttca acaagaagat cggcacggta ttgcgtcagt ttgccgtcgg
     3001 caaacgcttt tttgtattga tccggatcgt gaggcccctc ggcatccatc agaacgactt
     3061 cgcgaatgga ctgcttgagt cggtcaagag tgaactcatc tcgggaaatg ccaatcaatc
     3121 tctcaagtcc gctgccgatg aacgcgtatc ggcgttccgg caagagcaaa cggtatggga
     3181 cgcccgaagt attctcaatt gcggcacgat agtccttgtc tgttcgacgc agttcctcaa
     3241 gagactcgtg aatgcgcttc tcttctatcg cgtaggcgag gaaacctgag gcagacctta
     3301 cgaaatgaag cccgttctcg ttccattgtt cgggggcctc gaaacgctcg tgaagcgcga
     3361 agcccgttct tgtccccttg cttttcaaag gtaccaagag gcaggagcgg gtttgcggca
     3421 aaaggacgcc tgcttgcctc agagcgttca tctccctggc atggctgttg tctgagaaag
     3481 caatatcgct tctaactcct gccgcagccg agaggtctat gggtgttcca acagaactct
     3541 tcttggaact ccgcttttcg cctccattgc tgatgtcgca tatacatcga atcctgtcat
     3601 ccgtttcgct gatccagaga ctcgctgttg aaccgagaga gaatgaagat gccctgcgaa
     3661 catactcttc gaagaccgcg tccagatcgt actgcttgca gcagatgctc tcggcaattg
     3721 ccaccag
//
LOCUS       DAOQUC010000212         3727 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_27986_length_3727_cov_2.983115, whole genome shotgun sequence.
ACCESSION   DAOQUC010000212 DAOQUC010000000
VERSION     DAOQUC010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3727
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27986_length_3727_cov_2.983115"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3727)
                     /locus_tag="PKH07_04905"
     CDS             complement(<1..>3727)
                     /locus_tag="PKH07_04905"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34320.1"
                     /translation="ENPRAWESLSRLYGRQLIHDKSSDMALRLARYYRKRGVRHEAEK
                     WFREALSHVASLSVQQELAELLWSTGSRQAALDEICLVAESLAGAQQSSESVSLVSRF
                     LEEDPRNTRLLLALAGLHERVGNREAQIETLLQAAESWREKEFLSKAHLVYQQIIRID
                     PVNEKAYEALASIHLERGMDGEAVKQYLSLAEQYKALDAAQADAAKLERVLTRVLELD
                     AQNASALKSLAAIYEQQASVKDRTKAREARDRQIAVLGQLVPALLRLNDLEGASASLD
                     RLRALSPQNPVIGKLDEEIQNRRVAVLPQEALLKMLSKARQLTSEGSIPQAIQQYEEI
                     LDAEPMHVAAIVALCDVLQRTQQSDRASKLLEEKGLAFLESGLLPQAQNCLERCCSLC
                     PDNMTVHQALARTFALQGMRPRAVEILLHVASVIEEQNVEQAIQVLREAESLAPDQMD
                     LRIRIAGLLGRAEQFEESRREYSLAIDTLEQNGRPDLAAAAIESLLQHACPSEMEQKL
                     TLQMRLARRLFELDRSERASKVALEVGEAALEFSHWDVARQAFEMLFSVPQVEPAYRS
                     LDNRERLAEAQKRIGAVQKASEQYNLIADECIGKGDLKRAFSAYQKIFALNPEDMSAL
                     ERLFELAIQMDEDALGVDYGLQLGRSLAAKGLLGKAETTYQQLLNLEQRRDQFNPEIY
                     RATTSFFLDKGDSDKAVGCLLDLALRYEQKHQTDLQQEVYENILSLKIDSPKVLVACQ
                     LLARLQESSGKTEQALATYQRLAEIFRAKENLSDFIDVFRHVLRLAPHEESHYKSMAQ
                     AYIHLRQTPEAIDFFEHLADSAAKAQDWQTALLAIRQLVEIRPEWHSYRQQEILYLQN
                     LGLTDEALDCAQESAEDFLKKGMPEEAILILEALLKGQPDQPRVRSQVDEIRGQLDSD
                     KRQQDRQRVLQEAAVLENQGNISGAIAAYEKAIRSGVPAATTLESLARLYSESARSGV
                     VEHVEKAIDLYSQLAQQAQRNRQTESACSHLRKACALDPERVGLLNALARAYSDAGDM
                     ERAVATFMKVADIHQAQGLLDSLEDDYRRILAIEPENLNIRGRLGSLLAERNCVDEAT
                     EQFWMQAQEYEKREVIPRAISALQRILTLKPDHLPARRRLVQLYLQKGDARASLEQQR
                     EVVRMLLVAGKPDEAIQECRTALELEPGNLLMREELASLYTQLGYIKEALAQRLEMLE
                     ICREKELVGPILEICETILKDHPDRTDVSERLVQTLLD"
BASE COUNT          850 a         1096 c          914 g          867 t
ORIGIN      
        1 gtccagaagt gtctgtacca aacgttcact gacgtcagta cggtcaggat gatccttgag
       61 aatcgtttcg catatttcga gaataggccc aacaagctcc ttctcacgac agatctcgag
      121 catctcaagt cgctgcgcca acgcttcttt gatgtatccc aactgcgtat aaagacttgc
      181 cagttcttcc cgcatcagaa gattgcctgg ctccaactcc agcgcagtcc gacactcctg
      241 aattgcctcg tcgggcttgc ccgccaccaa caacattcga accacttctc gctgctgctc
      301 caggctggca cgcgcgtcac ctttctgaag gtacaactgc accaaccgac gacgagccgg
      361 aagatgatcg ggcttcagcg tcagaatgcg ttgcagcgca ctgatggcac gcgggatgac
      421 ctcgcgcttc tcgtattcct gtgcctgcat ccagaactgc tctgtggcct cgtccacaca
      481 gtttctctcg gccaaaaggc tgccaaggcg tccacggata ttgagattct caggttcaat
      541 cgcgagaatt cttcggtagt catcttccag ggaatctagc aagccctgtg cctgatgaat
      601 gtccgccact ttcatgaagg tcgcaacggc acgctccata tctcccgcat ctgaatacgc
      661 tcttgccaaa gcgttcagaa gtccaacacg ctcaggatcc agagcacagg ccttgcgcag
      721 atgtgagcat gcagattctg tctgccgatt tcgttgtgcc tgctgtgcaa gttgggaata
      781 cagatcgatt gccttctcaa cgtgctccac aacaccggat cgagcactct cagagtataa
      841 acgcgctagg ctttccagtg ttgtggcagc aggaacaccg cttcgaatcg ccttctcgta
      901 ggccgctatc gccccagaga tattgccctg attctccagc acggcggctt cctgaagcac
      961 tctttgtcga tcttgctggc gtttgtcact gtccaactgg cctcgaatct catcaacctg
     1021 actgcgaact ctcggctgat ccggctgtcc cttaagcaat gcttcaagaa tgaggatcgc
     1081 ttcttccggc attcctttct tgagaaagtc ttccgcgctt tcctgcgcac aatcgagcgc
     1141 ttcgtcggtc aatcccaagt tctggagata gaggatttcc tgttggcgat aggaatgcca
     1201 ttcgggacga atttccacta actgcctgat cgccaaaagc gccgtctgcc agtcctgagc
     1261 cttggccgca ctgtcggcca agtgctcaaa gaaatcaatg gcttccgggg tctgacgcag
     1321 gtgaatataa gcctgagcca tgctcttata gtgagactcc tcgtgcggag ccagacgcag
     1381 cacatggcgg aaaacgtcaa tgaaatcgga aaggttctcc tttgcccgaa agatctctgc
     1441 caaccgttga tatgttgcaa gcgcctgctc cgtcttgcca cttgactcct gaagtctggc
     1501 cagcagctga caggcaacca agaccttcgg gctatctatc ttgagagaga ggatgttctc
     1561 gtagacttcc tgttgcagat ccgtttggtg cttctgttcg tatcgcaatg caagatcgag
     1621 caagcagcca acggctttat ctgaatcccc tttgtccagg aagaacgaag tcgttgcccg
     1681 atatatctcg gggttaaact gatcgcgacg ttgctccaaa ttcaaaagct gctggtacgt
     1741 ggtctctgct tttccaagaa gacccttggc cgccaatgaa cgccccagtt ggagaccata
     1801 gtccacacca agtgcgtcct catccatctg aatggccagc tcaaaaagac gttccagggc
     1861 ggacatatcc tccggattca gggcaaatat cttctggtag gctgagaaag cgcgcttcaa
     1921 atctcccttt ccgatacact catcggcgat gagattgtac tgctccgatg ctttctgaac
     1981 agccccaata cgcttctgag cttccgcgag acgctcgcgg ttatccagac tgcgataggc
     2041 cggttcgact tgcgggacgg agaacagcat ctcaaaagcc tgtcgcgcca catcccaatg
     2101 cgaaaactcg agcgctgcct ctcccacttc caatgccacc ttcgaggcgc gttcggaacg
     2161 gtcgagttcg aacagtcttc tcgcaagacg catctgaaga gtgagtttct gctccatctc
     2221 cgaaggacac gcatgttgaa gcaacgactc gattgccgcg gcggccaagt ctggccgtcc
     2281 gttctgttca agagtgtcta tcgccagaga gtattcacgg cgagactctt caaattgctc
     2341 ggcacgtccc aacaagccag caattcggat tctcaaatcc atctgatccg gagcaaggct
     2401 ctctgcctca cgaagaacct gtatcgcctg ttcaacgttc tgttcctcaa tgacgctcgc
     2461 aacatgaagg aggatttcaa ccgccctcgg tctcatgccc tgcaaggcaa aggtgcgcgc
     2521 caacgcctga tgaaccgtca tgttgtcggg acacaaagaa cagcatcgct caaggcagtt
     2581 ctgtgcctga ggaagaagac ctgattcaag aaacgccaga cccttctcct caagcagttt
     2641 cgaggcacgg tctgactgct gagtacgctg cagaacatca cacaaggcaa caatggcagc
     2701 cacgtgcatc ggctcggcat caagaatctc ctcatactgc tgaattgcct gcggaatgct
     2761 accctctgaa gtcagttgcc tggctttcga caacatcttc agcagcgctt cctgtggcag
     2821 caccgcgaca cggcgattct gaatctcctc atccagtttg cctatgacag gattctgggg
     2881 actcaatgca cgcaagcgat ccaaagaggc tgaagcgcct tccagatcat tgagtcgaag
     2941 cagagcggga accaactgcc caagcaccgc gatctgacga tcacgcgctt ctcgtgcctt
     3001 ggttctatct ttgacggaag cctgctgctc gtaaatggct gcaaggctct tgagtgcgga
     3061 tgcattctga gcgtccagtt ccagaactcg ggtgagaact ctctccaact tcgcagcatc
     3121 cgcttgagca gcatccaagg ctttgtactg ttcggcaagg ctcaagtatt gcttgaccgc
     3181 ctcaccatcc atcccacgct caagatggat cgaggcgagc gcctcatatg ctttctcatt
     3241 gacaggatca atgcggatga tctgttgata gaccaaatgt gctttgctca gaaactcctt
     3301 ttcacgccag ctttccgcgg cctgcaagag cgtctcgatc tgggcttccc gattgccaac
     3361 acgctcatgc agaccggcaa gcgccaagag aagtcttgta tttcgtggat cttcttcaag
     3421 aaagcgactc acaagactca cggattcgga agactgctga gccccggcaa gggattcggc
     3481 aaccaggcag atctcatcga gcgccgcctg ccttgatccg gtgctccaaa gcagttcagc
     3541 aagttcctgt tgcaccgata gtgaggcgac atgggacaac gcttcacgaa accatttctc
     3601 cgcttcatgg cgcacaccgc gtttgcgata gtaacgggcg agtcgcagtg ccatgtcgga
     3661 tgacttgtcg tgaatcagct gacgaccgta aagacgagag agagattccc acgcccttgg
     3721 attctct
//
LOCUS       DAOQUC010000213         3713 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_28134_length_3713_cov_3.320667, whole genome shotgun sequence.
ACCESSION   DAOQUC010000213 DAOQUC010000000
VERSION     DAOQUC010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3713
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28134_length_3713_cov_3.320667"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="PKH07_04910"
     CDS             <1..190
                     /locus_tag="PKH07_04910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34321.1"
                     /translation="LQPDLLILDDPFAGLGTKFQNRLTEFLLSLEATVLVTSCLDEPL
                     KAFCDTFVLLQSKGRVER"
     gene            231..710
                     /locus_tag="PKH07_04915"
     CDS             231..710
                     /locus_tag="PKH07_04915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34322.1"
                     /translation="MQSEDKKQDTPSDVRWPAFLCFFLFLALAVLLPLSALDSGFRDR
                     LGKVAQLALFHYVLWAYIGYLWGPARVLRSEVPEPTLVVLFCILWFQTALFNTAILAM
                     EWRKGMVFLASLRSGVSVSIVLLGLPFILALCGLAGMILWLVLGYQNGSTKRHPSHP"
     gene            676..2487
                     /gene="uvrC"
                     /locus_tag="PKH07_04920"
     CDS             676..2487
                     /gene="uvrC"
                     /locus_tag="PKH07_04920"
                     /inference="COORDINATES: protein motif:HMM:NF001824.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HNT34323.1"
                     /translation="MVQPNDTHLIPEQPGVYLMKGEGEKVLYVGKAGNLRTRVRAHIQ
                     GTPGSGGFYRSFVPLVRDIAYLVTDNEVEALLLEYNLIKEHDPEFNVRLKDDKRYPYI
                     KVTLAEPFPRVFLTRTLAQDGSRYFGPYPHVKEARTTLNALKELFPIRQCRVPCERLR
                     RRTRPCLNYEMRKCLGPCSGKITREEYDVLVKGIVDFLSGRTRSVLSLAERKMKDYSA
                     LQHYEKAAVFRDILRAIEATSTQQRVSGALTESEDYIALDSVAEMLCVSVLRNRQGRL
                     VGGEYFFLDNAEHAETPERIRAFLRDFYALSTDIPPEIVLPVELEEQASLEEWLRSHS
                     GHGVTLHCPQRGRKVRVLELARQNAHFRAQERYLKMHGLHEEVDSGVIQIQKILGMPH
                     PPMRIECFDIANISGRDAVGSMVVFQNGAPAKSAYRRFKIQRTDQPDDYAMMREMLER
                     RFQHTDEVFGTMPDLVLIDGGKGHLNVALGVLQKYSLPIPALALAKQEEDLYLPDQDY
                     PLALDRGEASLKLLVSLRDEAHRFAQDYHHRVRRKSSQFSFLDGVSGIGPSRRKALLK
                     AFGSIKAIREATVEQLTSISGITEDMAMRIQEHLKKR"
     gene            2484..3152
                     /locus_tag="PKH07_04925"
     CDS             2484..3152
                     /locus_tag="PKH07_04925"
                     /inference="COORDINATES: protein motif:HMM:NF015961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNT34324.1"
                     /translation="MKWKEIRHRLILFVLPVLAWILRILLATLRMRVVGQEYLDEVAS
                     SDHGAIFVFWHSRILYMLVAGGTKIRVHTLVSMNRDGEYIARVAASFGRPTVRGSSSR
                     GGAVALMNMARILESGDSVAFTPDGPRGPREVFQAGAVHLSLRTGRPIVPAAYSARWK
                     KRFASWDGFVLPYPFSRGVFIVGAPLWPEDFPKEEQACELMRQAAESALRQLTVEADR
                     MVES"
     gene            complement(3439..>3713)
                     /locus_tag="PKH07_04930"
     CDS             complement(3439..>3713)
                     /locus_tag="PKH07_04930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34325.1"
                     /translation="VHAVGDAKVAPNTPGTSALFGWDIARDLECVYDESGYTVEGLVV
                     LDGMGGLYTSGNASVGAAPVFADDIAKDMELSPFFTFVTDAVVSGP"
BASE COUNT          839 a          968 c         1032 g          874 t
ORIGIN      
        1 ccttcagccc gatcttctga ttctcgacga tccgtttgct ggactgggaa caaagtttca
       61 aaaccgactc acggagtttc tgctctccct tgaagcgacg gtgttggtca ccagttgtct
      121 tgacgagccg ctcaaagcgt tctgtgatac ctttgtcctg ctgcaaagca agggcagagt
      181 cgaacgatga gagacagcac cgtcttgggg tctcggtacg aaagaacgcc atgcagtctg
      241 aagacaaaaa gcaagacaca cccagtgatg ttcgatggcc tgcgtttctc tgctttttcc
      301 tttttctggc gctggcggtg cttttgcctc tgagtgcctt ggattcgggt ttcagagacc
      361 gcctgggaaa ggtcgcacaa ctggcactct ttcactatgt gctttgggcg tatatcggct
      421 atttgtgggg acccgccaga gtgctgcgtt ctgaggttcc ggaacccact ttggtggtgc
      481 tcttctgcat tctctggttt cagaccgcgc tgttcaacac ggcgatcctg gccatggaat
      541 ggcgcaaagg gatggtgttt ctggcatcgc ttcggtcggg agtgagtgtt tccatagtgc
      601 tgctcggact gcccttcatc ctggcactgt gtggtctggc aggcatgatc ctgtggcttg
      661 tcctgggata tcaaaatggt tcaaccaaac gacacccatc tcatccctga acagcccggc
      721 gtctatctga tgaagggcga gggagagaaa gtcctctacg tcggcaaggc gggcaacctt
      781 cgcacccgtg tgcgtgccca catccaaggc acacccggca gcggaggatt ctatcggtcg
      841 tttgtcccat tggttcggga cattgcctat ctggttacag acaatgaggt ggaagctctt
      901 cttctcgaat acaatctgat caaggaacat gatccggaat tcaatgtcag acttaaggat
      961 gacaagcgct acccctatat caaggtcacc ctggccgaac cctttccccg cgtttttctg
     1021 acccgaacgc tggcgcagga cggcagtcgc tactttggac cgtatccgca cgtcaaagag
     1081 gcacgaacaa ctctgaatgc cttgaaggaa ctgtttccga ttcggcaatg ccgagttcca
     1141 tgcgagcgcc ttcgtcggag aactcgtccg tgtttgaact acgaaatgcg aaagtgcctg
     1201 ggaccctgtt cgggcaagat cacacgtgag gaatacgatg ttctcgtcaa gggcattgtg
     1261 gattttctga gtggacgcac aagatcggtt ttgtccctgg cggagcggaa gatgaaagat
     1321 tattcggcgc tccagcacta tgagaaggcc gctgtttttc gggacatcct tcgcgccatc
     1381 gaggcgacat caacgcaaca acgtgtctcc ggcgcgttga cggaaagcga ggactacatc
     1441 gcgctggatt ctgttgccga gatgctgtgt gtttcggttc tgaggaacag acagggacgt
     1501 cttgtcggcg gtgaatactt ctttctcgac aatgccgaac atgccgaaac tcccgaacgc
     1561 attcgcgcgt tcctgcgtga tttctacgcc ctttcgaccg acattccacc ggagattgtc
     1621 ctgcccgtgg aactggagga gcaggcttca ttggaggagt ggctcagatc tcattcaggt
     1681 catggagtga cactgcactg tccccagagg ggaagaaagg ttcgggttct tgaactggca
     1741 agacaaaatg cccacttccg tgcccaggaa cgatacctca agatgcacgg tctgcatgaa
     1801 gaagtggatt cgggtgtcat tcagattcag aaaatccttg gcatgcctca tcccccgatg
     1861 cgcatcgagt gcttcgacat cgccaacatc agtggcagag atgcggtcgg atccatggtc
     1921 gtctttcaaa atggtgcacc tgccaaaagc gcctatcggc gcttcaagat tcagcgaacc
     1981 gatcaaccgg atgactatgc catgatgcgt gagatgctcg aaagacgatt ccaacatacg
     2041 gacgaggtgt ttgggacaat gccggatctc gttctcatag atggaggcaa gggtcatctg
     2101 aacgttgctt tgggagttct tcagaagtac agccttccga ttccagccct cgcgcttgcc
     2161 aaacaggagg aagacctcta tcttcccgat caggattatc ctcttgcgct ggatcggggt
     2221 gaagcctcct tgaaattgct ggtgagtctt cgggatgaag cccaccgatt cgcgcaagac
     2281 tatcatcaca gagtgcgccg gaaatcgtcg cagttctctt tcttggatgg ggtttccggt
     2341 atcggtccgt cgcgcagaaa ggcgctgttg aaagcgtttg gctccatcaa agcaatacgg
     2401 gaagccactg tggagcagct gacatccatc tcgggcatta ccgaggatat ggcgatgagg
     2461 atacaggaac atctgaagaa gagatgaagt ggaaagagat tcgacatagg ctgattctgt
     2521 ttgtcctgcc ggtgctggca tggatcctca gaattcttct ggccaccttg cggatgcggg
     2581 tggtgggtca ggagtatctg gatgaggttg ccagttccga ccacggggcg atctttgtct
     2641 tctggcacag tcgaattctc tacatgttgg ttgccggagg cacaaagata cgagtccata
     2701 ccctggtgag tatgaaccgg gatggagaat acattgcccg cgtggcggca tcctttggac
     2761 gtccgactgt ccgaggttcc tcttcgcggg gaggcgccgt ggcgctgatg aacatggcgc
     2821 gtattctgga atcaggagac tccgtcgcgt ttacaccaga tgggccgaga ggaccgcgtg
     2881 aagtgttcca ggcaggagcc gtgcatttgt cccttagaac gggcagacca atcgtacccg
     2941 ctgcctacag cgctcgatgg aaaaagcgct ttgccagttg ggatggtttt gtgctgccct
     3001 atcctttcag ccgcggagtc ttcatcgtcg gtgccccgct ttggcctgaa gatttcccca
     3061 aagaggaaca agcctgtgaa ctcatgcgac aggctgcaga atcggcactt cgacagttga
     3121 cggttgaggc tgaccggatg gttgaatcgt agatattcct ttgtaccaga caccatcaag
     3181 agaatcgttc ctctgcaaaa cgatgagatt ccacattata gagtgaggtt ttggggcggg
     3241 actcgcgatc tcgcaatgac tccccgaaat gatcccgctc ggctactgca gtggaacggg
     3301 ttcttgctct tgcggatgcc tatctatcca gaacagcaaa aaatagagag cggggcagat
     3361 cgaaacctgc cccgcgcata gtcctcaaga attgcaccag ctggttgcaa tacttttgac
     3421 aacagaaatg gcaaccaact atggacctga aacaactgcg tcggtgacga atgtgaagaa
     3481 tggagacaac tccatatcct tcgcgatatc gtcagcaaac acaggagctg cgcccacaga
     3541 agcattaccg gatgtataga gaccacccat gccatcgagc acgacaagac cttcaacggt
     3601 ataaccgctt tcgtcataga cgcactccaa atctctggcg atgtcccaac cgaacagggc
     3661 acttgtgccc ggggtgttcg gagccacttt ggcatcacca actgcatgga cag
//
LOCUS       DAOQUC010000214         3713 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_28137_length_3713_cov_2.940131, whole genome shotgun sequence.
ACCESSION   DAOQUC010000214 DAOQUC010000000
VERSION     DAOQUC010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3713
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28137_length_3713_cov_2.940131"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..552
                     /locus_tag="PKH07_04935"
     CDS             <1..552
                     /locus_tag="PKH07_04935"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNT34326.1"
                     /translation="ENQMAAAAARGDQAAFNELIRLCSRTIYNVAYRIVLNEQDALDV
                     TQNVYYRIASKLGDFRATGSFRSWTAAIAAREAINHVRSPHHREIATEQETLELVSET
                     DAGRSAANPRELAVENEQREIILEAMGSLSEQQRGILTLRLLEGLGPKEIASVLDIPA
                     TQVRSQTHRAMDRIKGFLKRTVE"
     gene            576..1199
                     /locus_tag="PKH07_04940"
     CDS             576..1199
                     /locus_tag="PKH07_04940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34327.1"
                     /translation="MNGKEKQDHLEDEQRLLGFLLGDLSPEDAALFEKRLQTDRALQA
                     LEKEYRDALERAKDWLGSETPGAEGISALHTPSLSHTHRENVSTRHARSDYWRKVTKR
                     VLAAAAMFLIGFLFGQGSRLSLDNEREPLAPSFKNESVNVERMAPTPARMEEPEAPMK
                     LVDASVRRQTVQPDGRVLVETVLKETGSQALWVVDGRFRVSEPLATP"
     gene            1317..3143
                     /locus_tag="PKH07_04945"
     CDS             1317..3143
                     /locus_tag="PKH07_04945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34328.1"
                     /translation="MKRVCSTLILGWLILAAQIGVCGDQVGMDNLATVDRPQTVTLLP
                     GETMTFGKVTLRFISEGKVPGTGQPGLQNDMVGMPLAPGMGMEMGMPMDMMHGVPQVT
                     YHLILETSGPAPLKLQLTSQANLAAYYGEVVVARLGLAGNQMRANLGIQNTEIPVTNA
                     PFKKIVYFSAYCPVQIGSWELQFSPEQHTFSDGTQCYQLFAYDRVSKTTMNIPVVEKT
                     ERQMGRFSLFMGESWNDSKTVVYEIRAEADYARCGGETYVDSLQLIGGTAESSLQDMA
                     DEYGITIEWVESDSYPGLLEYCRALPFSSVRSQGLLRELLDTYLGYSLVFEWKDPYHL
                     RVSGNPQHFQIMRSKEEGARNLDEITAIFEKDYQTVTKIIPVTKMGAEALRAFIEPQL
                     SSYALVDGNSPQKNVLLQKAPYRIVASTGEFRTHTLRLSEERCISDPKTDSLLIQARP
                     STIARIEQALEDVYRLIDEGKKQSEPVSRFQVEIILLQAASSDNKSSKSSDLASFGLT
                     EDDLKPFGLDGVSKLGRGMVTLIGERSELGQARVALTDLYSCALQFQDFRKPYVVVKG
                     SLMDQNSNSIVLENTLYLEPLKPIVLGITNLKEALILVLRLK"
     gene            3231..>3713
                     /locus_tag="PKH07_04950"
     CDS             3231..>3713
                     /locus_tag="PKH07_04950"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNT34329.1"
                     /translation="MPTSGIHLSVIVPAYNEENRLGSTLERITEYLGQQDYTSEIVVV
                     DDGSQDRTSEVAKAVSPDILVIRNEPNRGKGYSVKRGMLAAHGDFRLFSDADLSTPIE
                     EVEKFWPFFDQGYDVVIASRALPGSQVEIHQNWFREMCGRAFNKVVKTIAMAQFSDTQ
                     C"
BASE COUNT          947 a          904 c         1063 g          799 t
ORIGIN      
        1 gagaatcaga tggctgccgc cgccgcaagg ggcgatcagg ctgcgttcaa tgagctgata
       61 cggctctgta gccgcaccat ctacaatgtc gcctaccgca tcgtactcaa cgagcaagac
      121 gctctggatg tgacacagaa tgtttactat cggatcgcat cgaaacttgg tgatttcaga
      181 gcaacgggct cctttcgttc ctggacagca gcgatcgcag caagagaggc aatcaaccac
      241 gttcgaagcc cacatcaccg ggagattgca acggagcagg aaacactcga actggtctca
      301 gagacagatg cgggtcggag cgcggcaaac cctcgcgagc tggcggtgga aaacgagcag
      361 agagagatta ttctggaggc aatggggagt ctctctgagc agcaacgagg cattttgacc
      421 cttcgccttt tggaagggct tggaccgaaa gagatcgcca gtgtgttgga tattcccgcc
      481 acgcaggttc gatctcagac tcatcgggct atggacagga taaagggatt cttgaagcgg
      541 acagtggagt gagttcttag acagaggaat gaagtatgaa cggcaaagag aaacaggacc
      601 atcttgagga tgagcaacgg cttcttggct ttctgttggg cgacctgagc cctgaggatg
      661 cagcgctgtt cgagaagcga ctgcagaccg acagagcgct acaggctttg gagaaagagt
      721 atcgggacgc actggaaagg gcaaaggact ggcttggatc cgagactcca ggtgcagaag
      781 gaatctcggc actgcacact ccaagtctct cacatacgca cagagagaac gtctcgacga
      841 gacatgcacg tagcgactat tggagaaagg tgaccaagag agttcttgct gctgcagcga
      901 tgtttctcat tggctttctg ttcggtcaag gttctcgctt gtccctggac aatgaacgag
      961 aacccctggc accgtctttc aagaacgagt cggtcaatgt ggaacggatg gctcccaccc
     1021 ctgcgcgcat ggaggagcct gaagcaccca tgaagctggt agatgcctcg gttcgacgac
     1081 agacggtgca accagacgga agagttctgg tagagacagt cttgaaggag acgggttcgc
     1141 aggctctgtg ggtcgtggat ggacggtttc gtgtatcgga acctcttgcg actccctgat
     1201 ttctgggaaa ccggactcgg gaaccggcgg ctcatttcgc acaagcacaa tgaaggagtg
     1261 ggcagagccc acgcgccagc aggtgaaaac ctgctctgca caaaggagag agggaaatga
     1321 aacgagtttg ttcaacactg attctgggat ggctcattct tgcggcacag attggagtat
     1381 gcggcgatca ggtcggcatg gataatcttg ccacggtaga taggcctcag acggtcacgt
     1441 tgctgcccgg cgagacgatg acgttcggta aggtcacact tcggtttatt tcagagggaa
     1501 aagtcccggg aacgggacaa cctggccttc agaacgatat ggttggaatg ccattggcac
     1561 caggcatggg aatggagatg ggcatgccaa tggacatgat gcatggggtg ccacaagtga
     1621 cataccatct catcctggag acgtccggac cggctccctt gaaactgcaa ctcacttcac
     1681 aggcgaacct ggcagcctac tacggtgaag tagttgtagc gaggctcggg ctcgcaggaa
     1741 accagatgag agcgaatctg ggtatacaga atacggaaat acccgtaacc aatgctccat
     1801 tcaagaagat cgtctatttc tcggcttact gtccagtgca gattggatcc tgggaactgc
     1861 agttttcacc ggaacaacac actttcagtg acggaaccca gtgttatcag ctgttcgctt
     1921 acgatcgtgt cagcaagaca acaatgaata tccctgtggt ggaaaagaca gaaagacaga
     1981 tgggaagatt ctctctgttc atgggagaga gctggaacga ttcgaaaacg gttgtctatg
     2041 aaatccgagc agaggcagac tatgcgcgtt gtggtggtga aacctacgtt gattccctgc
     2101 agttgattgg aggtacagca gagagttctc tccaggacat ggctgacgaa tatggaataa
     2161 cgattgagtg ggtggaatca gacagttacc cagggctgtt ggaatactgc agagcattgc
     2221 ccttcagcag cgttcgatcc caaggtctgt tgcgggaact gctcgatacc taccttggat
     2281 attcccttgt ctttgaatgg aaagatccct atcacctgcg ggtctccggg aatccacaac
     2341 acttccagat catgagatcc aaagaggaag gtgccaggaa ccttgatgag atcacggcca
     2401 tctttgagaa agactatcag acggtgacca agatcatccc cgtcaccaag atgggtgcag
     2461 aagcgcttcg cgcgttcatc gagccgcagc tttccagtta cgcactggtg gatggaaact
     2521 cgccacagaa gaatgttctt ctgcagaaag cgccctacag gatagtggcc tccacgggag
     2581 agtttcggac acatactctg cgcttgagtg aagaacggtg catctctgac cccaagacag
     2641 attcgctgct gatccaggca aggccttcaa ccattgcccg tattgaacag gcgcttgagg
     2701 acgtgtaccg gctcattgat gaaggcaaaa agcagagcga gccagtgtca cgatttcaag
     2761 tggaaatcat cctgctccaa gcagcgtcct ctgacaacaa gtccagcaag tcatcggatc
     2821 tggcttcttt cggactcacc gaagatgatc tgaagccgtt tggcttggac ggtgtctcca
     2881 aactgggtcg gggcatggtg actctgatcg gagagcgcag tgaattgggt caggctcgtg
     2941 tcgcactgac tgatctgtat tcgtgtgcgc ttcagttcca ggacttccga aaaccctatg
     3001 tcgttgttaa gggaagtctc atggatcaga actccaatag tatcgttctc gagaacacgc
     3061 tctatctgga gccattgaaa cccatagttc tgggcatcac gaatctgaaa gaggcactga
     3121 tcctcgtgct gcggctcaaa taagcgccgc agaacgttcc ggttgacaca atcgtccctt
     3181 ccagtgtacc tatacgcggt agaacactat gaaatgacag ggggaaagag gtgcccacat
     3241 ccgggatcca tctttccgtt atagtgcccg cgtacaacga agagaatcgt cttggaagta
     3301 cgttggaacg gattacggaa tacctaggtc aacaggacta cacatcggag atcgtggttg
     3361 tggatgacgg cagccaggat cgaacttccg aagtggcaaa ggctgtcagc cccgatatcc
     3421 tcgttattcg caacgagccc aatcgaggca agggctattc tgtcaaaagg ggaatgttgg
     3481 ccgcacacgg tgatttccgc ctcttcagcg atgccgatct ttccacgccc attgaagagg
     3541 tggagaaatt ctggccgttc tttgaccagg gatacgacgt cgtgatcgcc tcaagagcgt
     3601 tgccaggctc tcaagtcgaa attcatcaga attggtttcg ggagatgtgc ggtcgggcat
     3661 tcaacaaggt ggtgaagact atcgccatgg cgcagttcag tgacacgcaa tgt
//
LOCUS       DAOQUC010000215         3706 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_28205_length_3706_cov_3.023555, whole genome shotgun sequence.
ACCESSION   DAOQUC010000215 DAOQUC010000000
VERSION     DAOQUC010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3706
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28205_length_3706_cov_3.023555"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..527
                     /locus_tag="PKH07_04955"
     CDS             <1..527
                     /locus_tag="PKH07_04955"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HNT34330.1"
                     /translation="ARNYGVYNRLLLGYYSGFGYGLQRAFVQFDLSSIPSNATIQEVQ
                     FALYQDVGAAHASSDQSHVVDIYRVTSSWAEGTQNGAAGSVCWNDQPSINTTSLGSLT
                     IAGVAGGTWREWTSAGLKSVVQGWVNGSTPNYGLCVRNSTANETDDGAHKFYSKEQGS
                     APPMIYVRYWVPAP"
     gene            736..2211
                     /locus_tag="PKH07_04960"
     CDS             736..2211
                     /locus_tag="PKH07_04960"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNT34331.1"
                     /translation="MITPSTIVSTVRQGRKVLLCIPPPGDTLKGSAVGGLLKTATSLI
                     PQGICHLASACRQAGYETRILDCQALEMSLEEAVNAVCLFNPDFVGISATTLSIIHAS
                     QFAREIKKRIPTVRIVVGGYHVSALPEETLKEFQSFDAGVVGEGERTLVELLDAWMAS
                     RALSGIAGLVFREETGIRVTPRRALIKDLDSLGLPAWDLLPRLSRHYGSSAQRYRVFP
                     VASVITSRGCPHQCTFCDRSVFGTTFRGHSAEYVMAMIRTLRDEHGIRSIVFNDDLFI
                     ANKTRLTQICEAMIHENLRMSWSCDGRIDRLDDDLLKLMKRAGCWQLAVGIESGDDAI
                     LSRLNKGFTVEQARQAVVRMKGAGMSVKGFFVMGTPGETEQSLEQTVQLIKRLPLDEI
                     QITLFTPFPGTDLFRAVVSQEGFTPRWDEMNTASVSYVPKGLTKGRLLHYQRQGYLQF
                     YFRPRQVLYQLGKLKRPRFAVKMLREAFCFLQYALRRQTTQ"
     gene            2228..>3706
                     /locus_tag="PKH07_04965"
     CDS             2228..>3706
                     /locus_tag="PKH07_04965"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNT34332.1"
                     /translation="MAKKKKSVQLKPAPVKADPSTAQYETSPTLETQKIGNLLSTPVG
                     MGALLIGVPLLVRLLHFLFMLPNPLFRTVVMMDASWYHQQALAIVSGDFAPPDGFFRP
                     PFYSFFLAFVYMVFKPTMNIMMVRLFQCCLGSLNCYLTFRLGERLFSRRVAWIGWIIM
                     AFYGPLFFFDNELLSPVWEVFFNLVLLLSLTFAEGNTSMKWWALAGFCLGMSSITRAN
                     ILPFLPVMVVWLVWRTKRWLAFEEWRRPALVAVVLALLPGVLVGVRNKVGYGQFIFIA
                     HQGGLTFHVSNNPQADGLSPILPGRPDFTQSDATRIVADETKNPAPSPGQVAEYFSRK
                     ATTFIKENPGRFLMLTLKRSYYFLFSSFEGANNIDFDSYAKDSVPKMLFFWRLGRFYF
                     PSGLVIPLMWCGLAMSLPRWRRLSILYLYLFLQFASFVFFFVVARFRLPSVPIWTLFA
                     GQALTAQIGNRNMTQTQFKVFAAALVLLVLLVNADPWGASVRS"
BASE COUNT          764 a          988 c         1034 g          920 t
ORIGIN      
        1 aggcgcgcaa ctatggtgtc tacaacagac tcctgctggg ctactactcc ggattcggct
       61 acggcttgca gcgggcgttc gtgcagtttg acctttccag cattccatcc aacgcgacga
      121 ttcaggaagt gcagtttgcc ctgtatcagg acgttggagc ggctcacgcc agcagcgatc
      181 agtcccacgt ggtggatatc taccgtgtga cgagcagctg ggcggaggga acacagaacg
      241 gcgctgccgg ttctgtctgc tggaacgatc agccttcgat caataccacg agccttggct
      301 cgctcaccat tgcaggtgtc gccggtggca cctggcgtga gtggacaagt gctggtctca
      361 agagcgtggt tcaaggctgg gtgaacggct cgactccaaa ctacggactc tgtgtgcgca
      421 acagcaccgc caacgagacc gacgatggag cccacaagtt ctactccaag gaacagggtt
      481 ctgcaccgcc gatgatctac gtgcggtact gggtgcccgc accctagttc gcaacgttga
      541 gccttcgctg aggggacttt ggagccccag cgagaaggat gacccagggg gctggcgagc
      601 gattcgtcag cccccttcgc tatcatgaag gacgttcacg cccaggactt caaacgagat
      661 gatgtcgcaa tccagtcgga caagtggtag gatgaatctg tcctttgatt ggcgcaccaa
      721 atgagttctt cagagatgat tacaccttca acgatagtct ccactgtccg ccagggacga
      781 aaggtactgc tttgtattcc acccccaggt gacacactca agggctctgc cgttggggga
      841 ctgctcaaga cggccacatc acttattccc caggggatct gtcaccttgc cagcgcctgt
      901 cgtcaggcgg gctatgagac aagaatcctc gattgtcagg cgcttgagat gtcgctcgaa
      961 gaagcggtca atgcggtctg tctcttcaac cccgattttg tcggtatctc agcgacaaca
     1021 ctctccatca ttcacgccag tcagtttgct cgtgagatta agaagagaat acccactgtc
     1081 cggatagtcg tgggtggcta tcatgtcagt gctctgcccg aagaaacgct caaggaattt
     1141 cagtcctttg acgcaggtgt agtaggagag ggcgagagaa cgctcgttga acttctggat
     1201 gcctggatgg ccagccgtgc gttgtcgggg attgccggtc tggtattcag agaggaaaca
     1261 gggattcggg tgacccctcg acgagccttg atcaaggatc tggacagtct cgggttgccc
     1321 gcctgggact tgttgccaag actgtctcga cactatggaa gctccgcaca gcgatacagg
     1381 gtattccccg tagcatccgt tatcacctca cgtggatgtc cacatcaatg cacattctgc
     1441 gatcggtcag tctttggaac aaccttccga ggacatagcg ccgaatacgt catggcaatg
     1501 atccggacac ttcgcgatga gcatggcatt cgcagcattg tgttcaatga cgacctgttc
     1561 atcgccaaca agacacgctt gacccaaatc tgtgaagcga tgatccatga gaatcttcgg
     1621 atgagttggt cgtgcgatgg aaggatagac cgtctcgatg atgatctgct caaactcatg
     1681 aaacgtgccg gttgttggca actggccgtt ggcattgagt ctggggatga cgccattctg
     1741 agtcggctca acaaagggtt caccgtcgag caagcccgcc aggcggttgt gcggatgaag
     1801 ggcgccggca tgagcgtcaa aggtttcttc gtgatgggaa cgcctggcga gaccgagcag
     1861 tctctcgagc agacggttca gctgatcaag cgtctgcctc tcgatgagat tcagatcact
     1921 ttgttcactc catttccagg aacggatctt ttccgagccg tggtttcgca agagggcttt
     1981 acgccgcgct gggatgagat gaatactgcc tctgtgtcct atgttccaaa ggggttgaca
     2041 aaaggacgtc ttctccatta tcaacggcag ggatatttgc agttttactt caggccgagg
     2101 caagtgctgt atcagttggg gaaactgaag cgaccgcggt tcgcggtgaa aatgcttcga
     2161 gaggcgttct gtttcctgca gtacgcgctt cggcgccaga ccacgcaata gcacgagggg
     2221 gcaacgcatg gcaaagaaaa agaagagcgt tcaactcaag cccgcacctg tcaaagcgga
     2281 tccgtccacc gcgcaatacg aaacctctcc gacattggag acccagaaga tcggaaatct
     2341 cttgagtacc ccagtgggaa tgggagcctt gctcatcggg gtgcctctgt tggtcaggct
     2401 tttgcacttc ctgttcatgc ttcccaatcc tctgttcagg acggttgtga tgatggatgc
     2461 cagttggtat caccagcagg cgcttgcgat tgtaagcggg gattttgcgc caccggacgg
     2521 tttcttcaga cccccgtttt attccttctt tcttgccttc gtgtacatgg tgttcaaacc
     2581 gacgatgaac attatgatgg ttcgactctt tcagtgttgt ttgggcagcc tgaattgcta
     2641 tctgaccttt cgtttgggag agaggttatt ctcccgaaga gtggcctgga tcggctggat
     2701 catcatggcc ttctatgggc cacttttctt tttcgacaat gagctgctct cgcctgtctg
     2761 ggaagtcttc ttcaacctcg tgctgcttct gtcgctgacc tttgcggaag gcaacacttc
     2821 gatgaaatgg tgggcactgg cggggttttg tctcgggatg tcaagcatca cccgcgccaa
     2881 catcctgccc tttctccccg taatggtcgt ctggctggtc tggcgcacca aacgatggtt
     2941 ggcttttgaa gaatggcggc gaccggcact tgtggctgtc gtattggcac tgcttccagg
     3001 tgttcttgtc ggcgttcgaa acaaagtcgg ctatgggcag ttcatcttca tcgcacatca
     3061 gggtggactg acattccatg tcagcaacaa tccgcaagcc gatggcttgt cgcccattct
     3121 cccaggaaga ccggatttca cgcagagtga cgccactaga attgttgctg atgaaaccaa
     3181 gaatcctgcg ccaagccccg gacaggtcgc ggagtatttc tcccgcaagg cgacgacgtt
     3241 tatcaaggaa aaccccggtc ggttcctgat gttgacactc aaacggtcat actactttct
     3301 cttctcctct ttcgagggtg ccaacaacat tgattttgac tcctatgcga aagattcggt
     3361 gccgaagatg cttttcttct ggcgcttggg gcgattctac ttcccttcgg gtcttgtgat
     3421 accactcatg tggtgcgggc ttgcgatgag tctgccccgt tggcgtcggc tgtccattct
     3481 gtacctgtat cttttcttgc agtttgcatc cttcgttttc ttctttgttg tggcaaggtt
     3541 tcggctgccc agcgtgccca tctggacgct ctttgcggga caggcattga cggctcagat
     3601 cggcaaccgc aatatgacgc aaactcagtt caaagtcttt gctgcggcgc ttgtcctcct
     3661 ggtgctcctg gtgaatgctg acccatgggg agcgtctgtt cgttcg
//
LOCUS       DAOQUC010000216         3703 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_28246_length_3703_cov_2.930099, whole genome shotgun sequence.
ACCESSION   DAOQUC010000216 DAOQUC010000000
VERSION     DAOQUC010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3703
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28246_length_3703_cov_2.930099"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..513)
                     /locus_tag="PKH07_04970"
     CDS             complement(<1..513)
                     /locus_tag="PKH07_04970"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNT34333.1"
                     /translation="MRVGFPIVLASLLTLLQVCVGMTPAEQVFRQYRSPNETAVVEGD
                     ATELLASSDEIDSATLDGLLRHAARNNPSLRAAFARWQAALEQIPQMRALPDPRLSYE
                     IMARVSPREHTVMLSQMFPWVGTLSLEQKMAEKEARIECTRFRILEREIFGRIKNDYY
                     EYYYLSRRIAL"
     gene            706..2115
                     /locus_tag="PKH07_04975"
     CDS             706..2115
                     /locus_tag="PKH07_04975"
                     /inference="COORDINATES: protein motif:HMM:NF025112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HNT34334.1"
                     /translation="MRFARDSVPEEVWRIASVLSDFEFYPIGGIIRDSILHAWSLGKT
                     DVSQAARGDWDFATSALPEVVAERMERVGFLALDVGIKHGTVVAVKEGRQYEITTFRK
                     DVTTDGRHAEVSFGCSLDEDVQRRDFTINTLALNLQSLQVIDLCRGLDDLQAGIIRAV
                     GEPEIRFREDHLRLLRAARMATLLNFQIHEKTWNALVACAPMIDQVSSERIRDEIMKM
                     LATAKPSTGVRMLQRSGLLAVIMPELDRCFGVAQNQYHSHDVGEHTLLSVDAISPRFP
                     LLRMIHLLHDIGKPSARAYFPDRDDYVFYGHEKIGAEMAVEVLKRLRFSNKEIEQAQI
                     LIAEHMFKLTDPCLGKRGLRRFLRRLGRENLDAYLRLRIADRIGNEKWQGTWEPGLRE
                     AIRSLREIFRDEDALHVTDLAINGDDLIALGLKPSPLFSRILNNLLEKVLDEPSLNNR
                     HDLLCLVQQCIQEEKSSKN"
     gene            complement(2186..3364)
                     /locus_tag="PKH07_04980"
     CDS             complement(2186..3364)
                     /locus_tag="PKH07_04980"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010853440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HNT34335.1"
                     /translation="MSHFALTVSLMLLLAFVGRLSDASEEGLTQQTLFVRGEGGYNIY
                     RIPSLIVTPRGSLLAFCEGREAGDSSDIDMLLRRSDDGGKTWSERQVVWDEGKNVCGN
                     PCPVVDSDTGIIWLLMTWNNSEDRDEQLHEGTGKDTRRPFVCHSSDDGHTWSQPMEIT
                     EGAKRDGWLWYATGPGVGIQLRHESHKGRLVIPCDYTDKDGFGSHVILSDDHGKTWRI
                     GGTVSGGANECQVVELRDGTLVLNMRMQHNGDGRRGLATSRDGGETWSELVLDATLVD
                     PICQASLIRYACSETDEGDVLLFSNPAAPPLPGKTKRERAQMTVRLSLDGGKTWPFAR
                     LLHEGFSAYSCLAVMPDGTIGCLYEGGETAYSEIVFARFALGWLKTISLEKQDSQANE
                     "
     gene            complement(3361..>3703)
                     /locus_tag="PKH07_04985"
     CDS             complement(3361..>3703)
                     /locus_tag="PKH07_04985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34336.1"
                     /translation="STDFKSLWDQFVMVIGLLGGGLGGLFILGVFSKRANSFGAVCGL
                     VVGGVMQYLAKQDPRLHGYLYAGIGLISCVVVGYFASCCRPERGKNLDGLTVYTMLSE
                     SGESKERNCCR"
BASE COUNT          889 a         1046 c          941 g          827 t
ORIGIN      
        1 aagcgcaatg cgtcggctca gatagtagta ctcgtaatag tcgttcttga tccgtccaaa
       61 gatctcacgc tccagaatcc tgaatcgagt gcattcgatt ctggcttcct tctcagccat
      121 cttctgctcc aaactcaagg ttccaaccca agggaacatc tgggacagca tcaccgtatg
      181 ttctctggga gaaacgcgcg ccataatctc gtaggacaaa cggggatcag ggagcgccct
      241 catctggggg atttgttcga gagcggcttg ccatcgtgca aatgccgccc gaagggatgg
      301 attgttccga gcagcgtgcc gaagcagccc gtcaagcgtg gcgctgtcaa tctcatccga
      361 cgaagcaaga agttctgtcg cgtcgccttc gactactgct gtctcgttcg gtgaacggta
      421 ctgccggaaa acctgttcgg ctggtgtcat cccgacacag acttgcagaa gagtcaggag
      481 acttgccagc acgatgggaa aaccaactcg catgataagc ctcacctcat ctccctggca
      541 tcacagtcct gcggcatgga ctttcagata tccggagata tctcgctgca caactccccg
      601 aacgctgcaa ttcagaatag ggagtctgca aagaaatgtc aaattgacga ctggcaagca
      661 gtgtggcagt tttacccagt gaaacacgac agaaaggata ctcaggtgcg tttcgcaaga
      721 gactcagtcc cagaggaagt ctggagaatc gcttcggtac tctccgactt cgagttctat
      781 cccatcggag gaattatccg cgattcgatt cttcacgctt ggtctttggg aaagacggac
      841 gtctcgcagg ctgctcgcgg cgattgggac tttgcgacct cggcattgcc cgaggttgtg
      901 gcagagcgta tggagagggt aggttttctc gctcttgatg tgggaatcaa acacggcaca
      961 gtcgtggcag tcaaggaagg ccgccagtat gagataacca catttcgaaa agatgtcacg
     1021 actgacggtc gccacgcaga agtctccttc ggctgttccc tcgatgagga tgttcagcgt
     1081 cgtgatttca cgatcaatac tttggctctg aaccttcaat cgcttcaggt gatcgacctc
     1141 tgccgtggcc tggacgatct tcaagccgga atcattcgcg ctgtcggaga accggagatc
     1201 cgattccgcg aggatcacct caggctgctg cgggcagcgc gaatggcaac gctcttgaac
     1261 tttcaaatcc atgagaagac atggaatgct ctggtagcgt gtgctccaat gatcgatcag
     1321 gtgtcgtcag agcggatacg agacgagatt atgaagatgc tggccacagc caaaccctcc
     1381 accggcgttc gaatgctgca gcgctccgga ctgctcgcag tgatcatgcc ggaactggat
     1441 cggtgttttg gtgtggcaca gaatcagtat cactcccatg atgtgggcga acatacactc
     1501 ctctctgttg atgccatctc cccacgtttt cctctgctca ggatgattca tctgcttcat
     1561 gacatcggca agccctccgc tcgagcctat tttcccgaca gagatgacta tgtcttctac
     1621 ggtcatgaga aaatcggcgc cgagatggcg gtcgaagtgc tcaagcgcct tcgcttttcc
     1681 aacaaggaaa tcgaacaggc gcagatcctg attgcggaac acatgttcaa attgaccgat
     1741 ccctgcctcg gaaagagagg gctcaggcga tttctgagaa ggttgggacg tgagaacctt
     1801 gacgcctatc ttcggctgcg tatcgctgat cgaatcggaa atgagaagtg gcagggcaca
     1861 tgggaaccag gactgcgcga agccattcgc tcacttcggg agatattcag agatgaagac
     1921 gcgctgcacg tgaccgatct cgccatcaat ggcgacgacc tgatcgcgct cggactcaaa
     1981 ccgagccctc tgttttcaag aatactcaac aacctcttgg aaaaagtcct tgacgaaccg
     2041 tccttgaaca accggcatga cttgctttgc cttgtgcaac agtgcatcca agaggagaaa
     2101 tcttccaaga actgataggt cttgcggact tcatctcaag aggtctgcag gacagaacac
     2161 gcccccattc tggattcata ttcattcact cgttcgcctg cgaatcctgt ttctctaaag
     2221 atattgtctt gagccatccc agtgcaaatc gggcaaagac gatctctgag tacgctgttt
     2281 ctccaccctc atagaggcag ccaatagtgc cgtcaggcat cacggccaga caggagtagg
     2341 cggagaagcc ttcgtgcaag agtcttgcga aaggccaggt cttcccccca tccagactca
     2401 gccgcaccgt catctgtgcg cgttctctct ttgtctttcc gggaagcggc ggagcggcag
     2461 gatttgagaa cagcaggaca tccccctcgt cggtctcaga acaggcgtac cggatgaggc
     2521 ttgcctgaca tatcggatca acgagcgttg catcgagaac cagttctgac cacgtttccc
     2581 caccatcccg actggtggca agaccgcgcc ttccatcccc attgtgctgc atcctcatgt
     2641 tcagtaccaa agttccatct cgaagttcca caacctgaca ttcgttggca ccaccggaga
     2701 cggttccgcc aatacgccag gtctttccat gatcgtcact gaggatgaca tgcgatccaa
     2761 acccatcttt gtccgtgtag tcacagggaa tgaccagacg gcctttgtga gactcatgtc
     2821 gcaactgaat gccaacgcca ggacccgtgg cataccacaa ccagccgtct cgttttgcac
     2881 cttcggtgat ctccatgggc tgcgaccagg tatgtccatc atcactggaa tggcagacaa
     2941 agggacgacg agtgtccttg cccgtgcctt catgcaattg ctcgtcgcgg tcttcgctgt
     3001 tgttccacgt catgagaagc cagatgatgc cagtgtcaga atcaacaacc ggacagggat
     3061 tcccgcagac attcttgcct tcgtcccaga ccacttgtct ttcactccat gtctttccgc
     3121 cgtcatccga tcgtcgaagc agcatatcaa tgtcgctgct gtcgccagcc tcacgacctt
     3181 cacagaaagc cagcagagat ccccgcggcg tcacaatcag agaggggatt cgatagatgt
     3241 tgtatccgcc ttcaccgcga acaaacaagg tttgttgagt cagaccctct tccgaagcat
     3301 ctgacaaccg accaacgaag gccaacagaa gcatgagtga tacagtaagg gcaaaatggc
     3361 tcatctgcag cagttcctct ccttggattc gcccgattct gaaagcatgg tgtagactgt
     3421 aagaccgtcc agattctttc ctcgttcagg tctgcagcaa ctggcaaagt aacccacaac
     3481 gacacaggat atgagtccaa tacccgcgta caagtacccg tgcagtcgtg gatcctgttt
     3541 ggcgagatac tgcattactc ctcccacgac aagtccgcaa accgcgccaa aggagttggc
     3601 tctctttgag aagacgccca agataaagag accgcccaag ccacctccaa gaagaccgat
     3661 gaccatgacg aactgatccc agagcgactt gaagtccgtc gaa
//
LOCUS       DAOQUC010000217         3691 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_28382_length_3691_cov_3.491199, whole genome shotgun sequence.
ACCESSION   DAOQUC010000217 DAOQUC010000000
VERSION     DAOQUC010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3691)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3691)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3691
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28382_length_3691_cov_3.491199"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..636
                     /locus_tag="PKH07_04990"
     CDS             <1..636
                     /locus_tag="PKH07_04990"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103118 -
                     UDP-3-O-(R-3-hydroxymyristoyl)-glucosamine
                     N-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HNT34337.1"
                     /translation="FIGDHVVLYPMSYIGAGSAIGDESVIHPRVTIGERVSVGARAII
                     HPGTSIGSEGMNNGDQSTNPALSVEIGDDIEIGSNVVVAKGRSEPTRIGSGTKIDNLV
                     QIGSDAQIGNCCIIVAQTGIGENVVLEDGVTLAGQASIRPGIRVGKGATIAAQSVVET
                     DVPAGQVYFGFPAKPHDQEMRIKVCVSKLPNLFKTVKELEKKLENRPEGST"
     gene            729..2033
                     /gene="lpxC"
                     /locus_tag="PKH07_04995"
     CDS             729..2033
                     /gene="lpxC"
                     /locus_tag="PKH07_04995"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: protein motif:HMM:TIGR00325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HNT34338.1"
                     /translation="MRKQQSIAGQVSLTGVGLHTGNASTVTFTPAEPNSGLVFVRVDL
                     PATPRIPVHADYANPEEKLHTALKVGDAEVKTVEHVLATAIGLGIDNMEIHVTSNEVP
                     ILDGSAKPFAEALVGVGFVEQDAPCSTMGISNQISLSEGDISLTATPADHLELTFTVD
                     YPNSGIGRQTVSFAITPEVFEKEIAPARTFCFRKDVELMRAAGLIKGGSLENALVIDD
                     DGTTSQSLRYSDEIARHKLLDLLGDLALVGKRLESHVHAVRSGHAWNARFAKKILQEV
                     RMKAVGNWPLKAPLDIEQIRAILPHRYPFLFVDRLIEVNEEGTRGVGIKNVTLNEWFF
                     QGHFPNKAVMPGVLILEAMAQVGGIILLAKEKNRGQLAFLLSMEDVKFRKPVVPGDQL
                     RLEAEIVKEKKRFGRCVGKAYVDGAVVAEAAINFALVDANES"
     gene            2058..2834
                     /gene="lpxA"
                     /locus_tag="PKH07_05000"
     CDS             2058..2834
                     /gene="lpxA"
                     /locus_tag="PKH07_05000"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HNT34339.1"
                     /translation="MPNIHPTAIVDPKAELADDVEIGPYSVIGPEVIIGAGTVIGPSV
                     FIEKRTTLGCRCKVSKGAVLGTDPQDLRFKDKKTYLVIGDDCVLREFVTVNVATSEEK
                     PTRLGNNIMMMAYSHIAHDCQIGNGVIMANCASLAGHIHMDDGVILGGLAAIHQFSIA
                     GKYCIIGGFSRVTLDILPFSMAAGCPCKIHGLNMVGLKRRGFSKERLSALHQAFKILL
                     RKGLSLPDATKRLQDEMGDNSDVQEIIAFIQRSKRGIARH"
     gene            2876..>3691
                     /locus_tag="PKH07_05005"
     CDS             2876..>3691
                     /locus_tag="PKH07_05005"
                     /inference="COORDINATES: protein motif:HMM:NF013959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrin alpha"
                     /protein_id="HNT34340.1"
                     /translation="MKTKRFVEIVAVCLVWVCLSAQSPATTWVDLDDAAHTAQASILL
                     GEARNSWLGDSYHLADLNGDNVMDIVLGAYRHSSPNGARSGRVYILYGPVAGVTEPTT
                     IDLANIPGDHLILDGDAAEELFGYSITTGDLNRDQVPDLIVSAPLGTYAQINRSGKVY
                     VFFGKAGQKIISDAKGPLAAGTVADCIIYGAGAYDSAGMEMICADFTRDGTDDLIITA
                     PFGTEEGTLANRAYVFSGVLEPTAQASFSLAEDYTFLIQNLPFNNTSNLNNSGL"
BASE COUNT          882 a          907 c         1081 g          821 t
ORIGIN      
        1 ttcattgggg atcacgtcgt gctttatccc atgtcgtaca ttggtgcggg cagcgccatt
       61 ggagatgaga gtgtcatcca tccgcgagtg acgattggag aacgggtgag cgtgggtgcc
      121 cgagcaatca tccatcccgg gacaagtatc ggcagtgaag ggatgaacaa tggagatcaa
      181 tccacaaatc ctgccctttc tgtagagatt ggtgatgata ttgagattgg ctccaatgtc
      241 gttgttgcca agggcaggag cgaaccgacc cggatcggct ctggcaccaa gatagacaat
      301 ctggttcaaa tcggttcgga cgctcagatc ggaaactgct gcatcattgt tgcccagacc
      361 gggatcgggg agaatgtggt cttggaagac ggagtgactc tggcgggaca ggcatccatt
      421 cgtccgggca ttcgtgttgg caaaggagcg actattgccg cgcagagtgt cgtagagacg
      481 gacgtcccgg cagggcaggt ctactttggc tttcctgcca aaccgcatga ccaggagatg
      541 agaatcaagg tttgcgtcag caagttgccg aaccttttta agacagtcaa agaactggag
      601 aagaaactgg agaatcgtcc cgaagggtca acctaagcaa gggttgtttg ctccaacagg
      661 cagacatcga acaaaggcca aatacacctg ggcaggacag acacgttcag taaagacgac
      721 agacaacgat gagaaaacaa cagagcatcg ccgggcaagt cagcctgacc ggagtcggtc
      781 tccacacggg gaacgcttct acggttacct tcacgccagc cgagccgaac tcgggtttgg
      841 tctttgtacg ggtggatctg cccgcaacgc cgcgcatccc cgttcatgca gactacgcaa
      901 acccagagga gaaactccat acggcgctga aggtcggcga cgctgaagtc aagactgtgg
      961 agcatgtttt ggcaacggcg attggcttgg gcattgacaa catggaaatc catgtcactt
     1021 ccaacgaggt cccgattctg gatgggagcg caaagccttt tgctgaggca ctcgtaggtg
     1081 tgggattcgt ggagcaggac gcgccctgct cgaccatggg catctcgaat cagatctcct
     1141 tgagtgaggg ggatatctcc cttacggcca ctcctgcaga ccatctggaa ctgacattca
     1201 ccgtggacta tcccaattca gggatcgggc gtcagaccgt gtccttcgcg attacgcctg
     1261 aagtgtttga gaaagagatc gccccagcac gaaccttctg ctttcgcaag gatgtggagt
     1321 tgatgcgggc tgccggtctc atcaagggtg gaagccttga gaatgcgctg gtcattgatg
     1381 atgacggaac gaccagtcag agcctgcgat attctgacga aatagcgcgt cacaaacttc
     1441 ttgacttgct tggtgacctt gcgttggtgg gcaaacgact tgaaagccac gtgcatgcag
     1501 tccgatcggg acatgcttgg aatgcacgct ttgcaaagaa aattctgcag gaagtgagga
     1561 tgaaggctgt gggtaactgg ccactgaagg caccgttgga catcgaacag attcgggcga
     1621 ttctgcccca ccgttatccc tttctcttcg tggatcgtct gatagaggtc aacgaggaag
     1681 gaaccagagg cgttgggatc aagaacgtga cgctgaatga atggtttttc cagggacatt
     1741 ttcccaacaa ggccgtcatg cccggggttc tgattctgga agcaatggcg caggtcggtg
     1801 gaatcatcct gctggcaaaa gagaagaaca gggggcaatt ggcgtttctg ctctccatgg
     1861 aagatgtgaa gttcagaaag cctgtcgtcc cgggcgatca gctccgcctt gaagcggaga
     1921 tcgtcaaaga gaaaaagcgc tttgggcgct gtgttggcaa ggcctatgtg gatggagccg
     1981 ttgtcgcgga agcagccatc aacttcgcct tggttgacgc gaatgagtct tagtccattt
     2041 caggaaggaa ccaaaggatg ccaaacatcc atccaacggc aatagtggat cccaaggcgg
     2101 aattggccga cgatgttgaa atcggcccct actccgttat cggacctgaa gtgatcattg
     2161 gtgcgggtac tgtcatcgga ccaagtgtct ttatcgagaa gcgcaccaca ctgggatgtc
     2221 gctgcaaggt cagcaaaggc gcggtcttgg ggactgatcc ccaggacttg cgtttcaaag
     2281 acaagaagac ctatctcgtc attggtgacg actgcgtctt gcgggagttt gtcactgtta
     2341 atgttgccac gtcggaagag aagccaaccc gactcggaaa caacatcatg atgatggcgt
     2401 actcacacat tgcgcatgac tgtcagattg gcaacggagt cattatggcc aactgtgcct
     2461 ctctggcggg acacattcac atggacgatg gggtcatcct gggtgggctt gcggcgattc
     2521 atcagttctc cattgcggga aagtattgta ttattggtgg gttttcccgt gtgacgctgg
     2581 atattctgcc ctttagtatg gcggcaggat gtccctgcaa aattcacggt ttgaacatgg
     2641 tcgggcttaa gagaaggggc ttctcaaagg aacgcctcag cgcgctccat caggccttca
     2701 agattcttct ccggaagggt ctgtcgttgc ctgacgcaac caagaggctc caggatgaaa
     2761 tgggcgacaa ctcggacgtg caggagatca tcgctttcat ccaacgatcc aagaggggca
     2821 tagcccggca ctagatagag cgcgagaacg cctagaaaaa tcagggggca ccgagatgaa
     2881 gacaaaacga tttgtcgaga tagttgcggt ttgtcttgta tgggtctgtc tgtcggctca
     2941 gtcgccggcg acgacctggg ttgatttgga tgatgcggca cacaccgcac aggcatccat
     3001 actgttgggc gaggcacgaa acagttggct gggagattcc tatcatctgg cggatttgaa
     3061 cggcgacaat gtcatggaca tcgtcttggg cgcctacaga cattccagcc cgaacggagc
     3121 ccgatccggt cgcgtctaca tcctctacgg tcctgttgcg ggtgttaccg aaccgacaac
     3181 gattgacctg gccaacattc caggggatca cctgattctt gacggtgatg cagccgagga
     3241 actctttggg tactcgatca caacgggtga cttgaataga gatcaggtgc ctgatctgat
     3301 tgtcagtgct ccattgggaa cctatgcaca gatcaaccgt tcgggaaagg tctacgtatt
     3361 ctttggcaag gccggtcaga agatcatcag tgatgccaag ggaccgttgg cggctggcac
     3421 tgttgccgac tgtatcatct atggtgcagg ggcatacgac agtgccggta tggagatgat
     3481 ctgtgctgac tttaccaggg acggaacgga tgatctgatc atcaccgcgc cgtttgggac
     3541 cgaagaaggg acactggcca atcgggctta tgtcttttcg ggagttctgg aaccgacagc
     3601 gcaggcttcc ttctctctcg ccgaggacta cacttttctg atccagaacc tgcctttcaa
     3661 taacacatca aacctgaaca attccggact t
//
LOCUS       DAOQUC010000218         3690 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_28395_length_3690_cov_3.729849, whole genome shotgun sequence.
ACCESSION   DAOQUC010000218 DAOQUC010000000
VERSION     DAOQUC010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3690
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28395_length_3690_cov_3.729849"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..668
                     /locus_tag="PKH07_05010"
     CDS             <1..668
                     /locus_tag="PKH07_05010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNT34341.1"
                     /translation="HRFDQDKQSLERLIKSGDYGALDYIVGRNTWNCRKFPAWGAFRY
                     KIPDTLLVEGTVHHFDIMRSLTGSDAKTVHALTWNPAWSDFAGDSQGLILIEMESGVR
                     VFYEGAKSNSTTLNTWGHDYWRAECEKATLILDNRKLCTMSDLSGEHKVVDVPLAEQP
                     VWMNSWLAELFVNWVNGGETPPNSLDDNIQCAALLFAAIESAHTGKIVPVQEYLERHL
                     KAV"
     gene            941..2275
                     /locus_tag="PKH07_05015"
     CDS             941..2275
                     /locus_tag="PKH07_05015"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNT34342.1"
                     /translation="MATIPAYLKQVLTEKLADSLTATLPDLTPRRVFGEICLSGKATA
                     VVGMRRAGKTTFLHQLRRERMEAGIHREALPWINFEDERLGELKASHLSFLVDEYARR
                     FSQSRERGIVAWFFDEIQVVEGWERFVRRLLDTDRTEVFVTGSSAALLSREIATALRG
                     RAWEVLMHPFSFAEVLLHSGEVPSGEIALLDSTSRAHLERSFQNWLRVGGFPEVQRVD
                     DLTRHRLLRDYVDVAILRDVVERHRVSNVEGLRWLVRHLLGNAAGKFSVEKFHAALKS
                     QGIAISKDTVHQLLAHLEDCFLIRTVWMESSSERQRMVNPRKAYPVDAGLIPVFQLSD
                     KANTGHALETAVLVELERRRKSVTYVRTEGGFEVDFLVRSTDGGLELIQVCADASDLL
                     TGERELRALVDAGKMFPRASKRLLTLTQDSMPADVPADVTAQPAYEWFLRDS"
     gene            complement(2366..3388)
                     /gene="ruvB"
                     /locus_tag="PKH07_05020"
     CDS             complement(2366..3388)
                     /gene="ruvB"
                     /locus_tag="PKH07_05020"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007143905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HNT34343.1"
                     /translation="MPDRIVCPEQNDDEARMEETLRPATLDDFIGQNQVKTMLRIAIE
                     AALRRRDVLDHVLLSGPPGLGKTTLAHIIANELGVSLKLTSGPVLDKKAALAGCLTEL
                     QRGQILFIDEVHRLNRLVEECLYPAMEDFKIEVVIGGEGAYANTVNLPLEPFTLVGAT
                     TRSGMLTGPLRDRFGVVCRLGYYNNDDIFAIVKRSARILSVTTDEEGAWEIARRSRRT
                     PRVANRLLRRVRDYAEVRADGNINRDVAREGLQMLEVDEVGLESLDRLILSAIIEKFD
                     GGPVGLKSLAVAVGEDVETIEEVYEPFLIQEGFMHRTPQGRVVTERGYAHMGHKPKER
                     DERTLF"
     gene            complement(3425..>3690)
                     /locus_tag="PKH07_05025"
     CDS             complement(3425..>3690)
                     /locus_tag="PKH07_05025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34344.1"
                     /translation="FYDQDIVMEPNDGEVLLDMRVTDSWIYLIAINQADRQVARTFHL
                     PRRLRPERVEVMFENRVFAKPKRQFVDTFEPWDVHVYRFSRPR"
BASE COUNT          800 a          972 c         1052 g          866 t
ORIGIN      
        1 gccatcggtt cgatcaggac aagcagagtc tggagcggct catcaagtcc ggcgactatg
       61 gcgctttgga ttacatcgtc ggtcgcaaca catggaactg ccgcaaattc ccagcatggg
      121 gagcgttccg ctataagatt cccgacacac ttctggtcga aggaacagtg catcattttg
      181 acatcatgcg ttctctgacc gggtcggatg ccaagacggt tcatgcgttg acctggaatc
      241 ccgcatggag tgattttgca ggcgattcac agggactcat cctcatcgaa atggaaagcg
      301 gcgttcgagt cttctacgaa ggagccaagt ccaattccac gacgctcaac acgtggggac
      361 acgactactg gcgcgccgag tgtgaaaagg caacgctgat attggacaac agaaagctct
      421 gtaccatgtc cgatttgtcc ggggagcaca aggtcgtgga cgttcccttg gcggaacagc
      481 ctgtctggat gaactcctgg ctggcagaac tgtttgtcaa ttgggtcaac ggtggcgaga
      541 cacctcccaa cagcttggac gacaacatcc agtgtgccgc gctgcttttt gcagctattg
      601 agtctgcaca tacagggaag atcgtccctg tgcaggaata tctcgaacgg cacctgaagg
      661 cagtttgatt cggttagttc actttggatt gtgcaatcgt ttgagactct aataggggtt
      721 cctctatccg catccagacg gatggacatc gtctccaatc gttctgctcg ttcgtagtgt
      781 ttctgcacgc ttcgggtgca acgctgccga agccctgtcg ttcgtacgcc ctgcttcggc
      841 ggcacgcaaa gccttgctac gggagcatcc gaaaacagat ttctccttgt aataaggaga
      901 aattagcagt atattctccc ttaaataagg agaacttaag atggcaacca ttccggccta
      961 tctgaaacaa gtgctgactg aaaagcttgc tgactctctg acagcgactc ttccggatct
     1021 taccccgcgg agagtttttg gtgagatctg cctgtctgga aaagcgacag cggttgtcgg
     1081 aatgcgccgt gcgggaaaga caaccttttt acatcagttg cgccgagagc ggatggaagc
     1141 ggggattcat cgagaagcac tgccgtggat caacttcgag gatgaacggc tcggagagtt
     1201 gaaggcatcg catctgtcct ttttggttga cgaatacgct cggcggtttt cccagtctcg
     1261 agagagggga attgtggctt ggttcttcga cgagatccaa gtggttgagg ggtgggagcg
     1321 ttttgttcgc cgtctgctgg acactgaccg aacggaagtt ttcgtcacgg ggtcttctgc
     1381 ggcgctgttg tctcgggaga tcgccactgc actgcgaggt cgcgcctggg aagttctgat
     1441 gcatcctttc agtttcgctg aggtgctact gcacagtgga gaagtgccat cgggtgagat
     1501 tgccttgctc gactcgacgt ccagagcaca ccttgagcgc tcattccaga actggctgcg
     1561 agtggggggc tttcctgagg ttcagagagt agacgacctg acgcgccatc ggctgctgag
     1621 ggactacgtg gacgttgcca tcctgcgtga cgtggtcgaa cgacaccgag tcagtaacgt
     1681 ggaggggttg cgttggctcg tccgtcatct cttgggcaac gcagcaggca aattcagtgt
     1741 ggagaaattc catgcagctc taaaatcgca ggggatcgcg atttcgaaag acaccgtcca
     1801 tcagctgctt gcgcatcttg aggactgttt tctcataaga acagtgtgga tggagtccag
     1861 ttcggagcgc caaaggatgg tcaatccacg gaaggcatat cctgttgatg ccggtctcat
     1921 tccagtcttc caactcagcg acaaagcgaa cacggggcac gcccttgaga ctgccgtgct
     1981 ggtcgaattg gagaggcgtc gcaagagcgt cacttatgtg cgcactgaag ggggcttcga
     2041 agtggatttt ctcgtgcgca gtactgacgg cggcttggaa ctgatacagg tgtgtgcaga
     2101 tgcctcagat ttattgactg gggagcggga actgcgtgcc ctcgttgacg caggaaagat
     2161 gttcccgcgc gcctcaaaac gtctactgac tctcactcag gacagtatgc ctgctgacgt
     2221 acccgcagat gtcacggcac aaccggcgta tgaatggttt ctgagggact cctaggaaac
     2281 cagacttcga acaagcctgt ttcttaccat gagcaagcgt ttgcaggagt gggcatagcc
     2341 cacgtgcctg caggtgctaa cctgctcaga atagcgttcg ttcgtcgcgt tccttgggtt
     2401 tgtgtcccat gtgggcgtag ccacgttcag tgaccactct gccttgtggg gtgcggtgca
     2461 tgaagccttc ctggatcaga aacggttcgt agacttcctc gatggtctcc acgtcttcac
     2521 cgaccgcgac cgcgagggat ttgagaccga ccggcccgcc atcaaatttc tcgatgattg
     2581 ccgagagaat cagacggtcg agtgattcca aaccgacctc gtccacctcg agcatctgga
     2641 gtccttcccg cgccacatca cggttgatgt ttccgtcggc gcggacttcg gcgtaatctc
     2701 gaacacggcg cagcagtcgg ttggccactc ggggagttcg tcgtgagcgt cgggcgattt
     2761 cccaggctcc ctcttcatcc gtggtgacag acagaatgcg ggcagaacgt ttgacgatgg
     2821 cgaatatgtc atcgttgttg taataaccca gccggcagac cacaccgaat cggtctcgca
     2881 aagggcctgt caacatgccc gagcgcgttg tcgcgccgac cagtgtgaat ggttccagag
     2941 gaagattgac ggtatttgca taagcccctt cacccccgat gaccacttcg atcttgaaat
     3001 cttccatcgc gggataaaga cattcctcaa cgaggcgatt gagacgatgg acttcatcaa
     3061 tgaaaagaat ctgtccccgc tgcagttctg tcaggcaacc ggcaagagct gccttcttgt
     3121 caagcaccgg accggaagtc agtttcagac tgacacccag ctcgttggca atgatatgcg
     3181 ccagtgttgt cttgcccagc ccagggggtc ctgagagaag cacgtgatcc aggacatccc
     3241 gacgtcgcag tgccgcttcg atggcaattc tcagcatggt cttgacttga ttctgaccga
     3301 tgaaatcatc gagagtcgct ggcctgagcg tctcttccat gcgggcttca tcatcattct
     3361 gttcgggaca aacgatgcgg tcgggcactc ctgtcctcct gcggaagaat tcagtcttca
     3421 gattctaccg ggggcgtgag aatctgtaaa catgcacatc ccaaggctca aatgtgtcaa
     3481 cgaattgacg cttcggtttg gcaaagactc ggttctcgaa catgacctca actctctccg
     3541 ggcgcagcct tcggggcaga tggaatgtcc ttgccacctg ccgatctgct tgattgatgg
     3601 cgatcaggta gatccaggag tcagtcacgc gcatgtcgag aagtacctcg ccgtcgttgg
     3661 gttccatcac aatgtcctgg tcatagaacg
//
LOCUS       DAOQUC010000219         3680 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_28501_length_3680_cov_3.641103, whole genome shotgun sequence.
ACCESSION   DAOQUC010000219 DAOQUC010000000
VERSION     DAOQUC010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3680
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28501_length_3680_cov_3.641103"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..612)
                     /locus_tag="PKH07_05030"
     CDS             complement(<1..612)
                     /locus_tag="PKH07_05030"
                     /inference="COORDINATES: protein motif:HMM:NF024909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HNT34345.1"
                     /translation="MFGSRGNKGRRQTQLVQAQLDRLSRLGLHARSVVEGTLMGLHRS
                     HQKGWNVEFAGFREYAPGDDPRHIDWKVLGRTDKLFLKEYEEETNLRAYLVLDRSNSM
                     NYGSKDATKFEYGVALASALAYLFIRQGDAVSMATFDNQIRDYLPPRSSPDHLQRVWR
                     LLESLSPADVTNVGDPLKELAGRVRAGCRGRPVERRLQRERGAS"
     gene            complement(786..1835)
                     /gene="mtnA"
                     /locus_tag="PKH07_05035"
     CDS             complement(786..1835)
                     /gene="mtnA"
                     /locus_tag="PKH07_05035"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018086583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HNT34346.1"
                     /translation="MSQVKTIEWKDGAIVLPDQTLLPERLEMLRCEKIQQIWEAIKML
                     RVRGAPALGVAAAYAVVLGLKEFAGSAREEFDRQMNQVCDYIATSRPTAVNLFWALER
                     MKRFRQTLAEQPTQLVWEALLEEARRIHQEDEQTCQAIGDHGQTLLPNPATVITHCNA
                     GALATAGIGTALGVIYAAQQVGKKVRVYSDETRPLLQGARLTAWELIQNGVEVITICD
                     NMAAVVLKTKSVDCVIVGADRIAANGDTANKIGTYGLSILAKHHGAPFYVAAPLSTID
                     RSLSNGAQIPIEERDPDEIRRGFGRLTAPPNVQVFNPAFDVTPAENVSAIITEKGILR
                     APYERSIAEAFKGRE"
     gene            complement(1858..3240)
                     /locus_tag="PKH07_05040"
     CDS             complement(1858..3240)
                     /locus_tag="PKH07_05040"
                     /inference="COORDINATES: protein motif:HMM:NF024873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HNT34347.1"
                     /translation="MTDRLSELERLRQALKSKSPNVAASLMTGRQVLERPKPTADDLP
                     SVIPGGFVSNDVGRFYLHEKFTPADKQRGRIPVSSLAGLSSGDLARLSPDRLLTESDK
                     PVVFLDTETTGLAGGTGTYAFLVGIGYWRGDGFVVEQYFMSDYDEEPALLTALSNIDE
                     RFSALVTYNGRTFDVPLLRSRFLMNRLRSPFDRLPHVDLLGSSRRIWSRSLESCSLSS
                     VEEQVLGIRRSGDVPGAIIPSLYFNFLKRKNPEPLKPVFSHNAQDILSLALLTWRVSE
                     MLANPLEVDMPPMDLFSVGRLMEQAQSDDVALACYERVRQSHPGTKAARLARLNLAVL
                     HKRLRRWDKCVPLWKEAFQDSKEGVPYRLYRDCAIYLEHVKRQHKNAIAVVQEALDRL
                     ELDIQLGRPATMVGSRDSYAVRADFLHRLLRLTRKRDKQPGTPLDEGRDEDISQKSDM
                     DDFDSDEQTE"
     gene            complement(3247..>3680)
                     /locus_tag="PKH07_05045"
     CDS             complement(3247..>3680)
                     /locus_tag="PKH07_05045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34348.1"
                     /translation="SDALLARPLRWILWGIFSLFPNPSDSVRSESSYWVYREKMLRKS
                     LHGLAETLPQLVYDRITLLLALLAFCAAVTSLVATGNLLWFLCIGLGMFCLIVSTLSL
                     KYGMKGWILAMILFTAMLGFFLEPKIVQTGLPGLYNLFQLW"
BASE COUNT          886 a         1070 c          921 g          803 t
ORIGIN      
        1 cgaggcacct cgctctcgct gaagacgacg ttcaacggga cgaccgcgac atcctgcacg
       61 aacacgaccc gccagttcct tcaatggatc gccgacattc gtcacatcag caggagacag
      121 cgactccaaa agtctccaga ctctctgcaa gtggtcgggt gatgatcgag gtggaagata
      181 atcgcgaatc tgattgtcaa aggtggccat actgactgcg tctccctgtc ggataaaaag
      241 atacgcgagt gctgaggcca aggccacccc atattcgaac ttggtagcat ccttggagcc
      301 ataattcatc gaattggagc gatccagaac cagataagca cgaaggttgg tttcctcttc
      361 atattccttg aggaagagtt tgtccgtcct tccaagaacc ttccagtcaa tgtgtcgcgg
      421 gtcatccccc ggagcatact cccgaaaacc ggcaaactcg acgttccagc ccttctgatg
      481 ggaacgatgc aaacccatga gcgttccctc gacgacggaa cgggcgtgaa gccccagccg
      541 cgacagccga tcgagctgcg cctgaaccag ttgcgtctgt ctgcgtcctt tgtttcctcg
      601 cgagccgaac acgatctcca agcttccttc tccgactgag acgaaactac atcacagccg
      661 gtcttgcaac gtttctgata gtattattcg aagtgactgc tattgcaaac gaattccagc
      721 gaagaatctg cgatagccat cagaaactgg cacagggaat ttggatcagt gcgatcccag
      781 gatcgtcact ccctgccctt gaacgcctcc gcgattgaac gttcgtacgg agcgcgaaga
      841 atcccctttt ctgtgatgat cgctgagaca ttctccgcag gggtcacgtc aaatgccgga
      901 ttgaaaacct gaacgttggg tggcgcagtc agtctcccga agccgcggcg gatctcatcc
      961 ggatcccgtt cttcaattgg aatctgagcg ccattcgaga ggcttcggtc aatcgtcgaa
     1021 agaggtgctg cgacatagaa cggcgcccca tgatgctttg ccaggattga gagcccatag
     1081 gtgccaatct tgtttgccgt atcgccgttg gctgcaattc gatctgctcc tacaatgacg
     1141 caatccacac tctttgtctt caacaccaca gccgccatgt tgtcgcagat cgtgatgacc
     1201 tcaacgccgt tttgtatgag ttcccaggcg gtaagtcttg cgccctgcag cagcggtcgc
     1261 gtttcatccg agtacactcg caccttcttg ccaacctgct gcgctgcata aatgacccca
     1321 agcgccgttc cgattcctgc tgttgccaag gctcccgcgt tgcagtgcgt gatgacagtc
     1381 gccggattcg gaagaagcgt ctgtccatga tctcctatgg cttggcaggt ttgttcgtct
     1441 tcttgatgaa tccgacgtgc ttcctcaagc aacgcttccc aaacgagctg ggtaggctgc
     1501 tcagcgagcg tctgacggaa acgcttcatt cgctccagcg cccagaaaag gttgaccgcg
     1561 gtaggtctgg aagtcgcgat gtagtcgcag acttgattca tctgtcggtc aaactcctct
     1621 cgggcactcc ccgcaaactc cttcaaaccc aataccacgg catacgccgc agcaacaccc
     1681 aatgccggag cacctcgaac ccgaagcatc ttgatcgctt cccaaatctg ctgaatcttc
     1741 tcgcatcgaa gcatctccag acgctctgga aggagtgtct gatccggcag tacaatagca
     1801 ccgtctttcc attcaattgt cttaacctga ctcatcggat ctccctgatt caatatgtca
     1861 ttcggtttgc tcatccgaat cgaaatcatc catatcggac ttctgagaaa tgtcttcgtc
     1921 tcgaccttcg tccaacgggg tacctggttg cttatcccgc tttcgcgtca gccggagcag
     1981 tcggtgaagg aaatccgctc tgaccgcgta agagtcccga gaacccacca tcgttgcagg
     2041 tctacccagc tggatgtcga gttccagacg atccaaagcc tcctggacaa cggcaatggc
     2101 attcttgtgt tgtcgcttca catgttcgag ataaatggca cagtcacgat agagccgata
     2161 gggaacaccc tctttggaat cctgaaacgc ttctttccaa agtggcacac acttgtccca
     2221 tcggcggagt cttttgtgta gaactgccag attcaggcga gccagtcttg ccgccttggt
     2281 gcctggatgg ctctgcctga cccgctcgta acaggcaagc gccacatcgt cactctgcgc
     2341 ctgttccatc aaacggccta ctgagaacaa gtccatcggt ggcatgtcca cctcaagggg
     2401 attcgcgagc atctctgaga ccctccacgt caacagagcc agagacaaaa tatcctgcgc
     2461 attgtgagag aacaccggtt tcaagggctc cggattcttt cttttcagaa agttgaagta
     2521 tagactgggg atgatcgccc caggcacgtc tccggagcga cggattccca gaacctgctc
     2581 ttctacggaa gacagactgc aactctccag actcctagac catattcttc gactcgaacc
     2641 aagcaggtca acatgcggca gccggtcaaa gggactccga aggcggttca tcagaaatcg
     2701 ggaacgcagc aagggcacgt cgaaggtgcg cccgttgtat gtcacgagag ccgagaaacg
     2761 ttcatctatg tttgaaagtg ccgtaagcag cgcaggttct tcgtcatagt cgctcatgaa
     2821 gtactgctcg acaacaaatc cgtctccacg ccagtaacca attccgacca gaaacgcata
     2881 ggtgccggta ccacccgcca gtccggtcgt ctccgtatca agaaaaacaa ccggtttgtc
     2941 cgattccgtc aggagacggt ctggactgag gcgcgccaaa tcacctgaac tcagcccggc
     3001 aagagatgaa acgggaatgc gtccgcgttg cttgtctgcc ggagtaaact tctcgtgaag
     3061 gtagaaacgc ccaacatcat tgctgacaaa accaccagga atgacgctgg gaagatcatc
     3121 ggcagtcggt ttcgggcgtt ccagaacttg tcgtcctgtc atgagagaag ccgcaacatt
     3181 cggcgacttg gacttcaatg cctgtcgcag tctctccaat tcggaaagcc tatcagtcac
     3241 tcacgatcac catagttgga acaggttgta tagtcccgga aggccagtct gaacgatctt
     3301 tggctcaaga aagaagccca gcattgcggt aaacaagatc atcgccagaa tccatccctt
     3361 catgccgtac ttcagagaca gcgtcgagac gatcagacaa aacatcccca gaccgatgca
     3421 aagaaaccac agaagattcc cggttgccac aaggctcgtc actgccgcac aaaaggcaag
     3481 cagtgcaagc agtagcgtga ttcgatcata gaccagttgc ggaagggtct ccgccagacc
     3541 gtgaagcgat ttgcgcagca tcttctccct gtagacccag taactgcttt cggaacggac
     3601 ggaatcggac gggttgggga agagggagaa gatcccccac agaatccacc gaagcggcct
     3661 tgccaacaag gcgtcagagg
//
LOCUS       DAOQUC010000220         3670 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_28624_length_3670_cov_2.886030, whole genome shotgun sequence.
ACCESSION   DAOQUC010000220 DAOQUC010000000
VERSION     DAOQUC010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3670
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28624_length_3670_cov_2.886030"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(256..1272)
                     /locus_tag="PKH07_05050"
     CDS             complement(256..1272)
                     /locus_tag="PKH07_05050"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HNT34349.1"
                     /translation="MKALILDGDGRSHIGEVPDPVAGEGEVLVAMRCSGICGSDLVYY
                     HMSSDQLDRMGNRIPCHEPSGVVVDIGTGVTSLQPGDRVSVYHYFSCGKCRECRRGQM
                     QWCAQTRGLGGPIQGSAADLMVVPERNAMKLPDSLSFVDGALIACGAGTSFSAMNKLQ
                     PSTRDTLLVLGLGPVGLCGVAMGKALGARVVAVGKRQPRLQLASLFGADVVIDIAQTE
                     NVPKAIREACKAPTLVYETSSAPQARQWAVDTCARGGKICTVAGRGEPGWPAGPIVGK
                     EITLQGSFVLPVWMVFDLIKFLVEKDLKLDRMVTHRFPIDRADEALALFETKECGKVV
                     IEWE"
     gene            complement(1454..2215)
                     /locus_tag="PKH07_05055"
     CDS             complement(1454..2215)
                     /locus_tag="PKH07_05055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34350.1"
                     /translation="MMSKTSAHVQRLVPALLGGFVLVAFVFVMALLSSAQQPASVQAM
                     PPLENSDLDGNGVIDHQDFRLLISVWHGLATPVPSATPTPTEMPTNTPTPVPDYVVDG
                     FWVGNFTEYFSDPANHPVNRTGQFLWHLKHSGSQVTGTGDDTQYPLTISAQFENGLMR
                     GTLKSFTTFQIWAVQDIPASVTSGYVPKFEGFMWGSKDGRNYDASFTVNRSSVRPTLA
                     PTKTPTPTRSATRTPDRTFTPTRTVTRTPTPTDEF"
     gene            complement(2215..>3670)
                     /locus_tag="PKH07_05060"
     CDS             complement(2215..>3670)
                     /locus_tag="PKH07_05060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34351.1"
                     /translation="MRYFVSAQTLRRDGFLILAGVCFCMLLSNVRAADLVDAYRPIAV
                     TIGDGATREFSTRLFSDINENSEYDPGEELKNISYDLDVVPPAPLGGVINRRAGYTDA
                     NGLFWFEYRTGTEAGAVEFNISSASTSLPIFPVWIEQVVPIRTEAGQDDLLERVANTP
                     ADVTPGRYVGLQLRNDDFFRLFVGEVDFVSISMYYLKETAGQSTFMHVLQGTEEVQSN
                     VGSPSGRIWCEFVPTPNTDYQIRVTGQTFSSGYSLSAMTQVGHSVHDLTTWNVAAPTP
                     PGPTWTPTSSPTNTPPYTPTETPTRTPTLTATPTFTAKPTPFGDANGDGVVDDRDLFV
                     MMWEYANQQSPTPTQTATRTPTPTRTGTPTRTPTSVMPNLGGIWRGSFKEFYEPNDPL
                     NKPFERDSGSMIWIVTSTGSTVTAEGMKTGKSGTREMTVSGTLNETVYMTGTLRMDFN
                     PTFTLHLINSTTLTGVYRGKAESTAVYGQLNLEK"
BASE COUNT          844 a          979 c          986 g          861 t
ORIGIN      
        1 gtcaggatgc acaatcgatc cgccagttct tcagtatgtt ttctcaacag atccagagat
       61 ccgaaaggcc tggtagacct ttcccagaac agcggcgcat cattgcgcgt acaaagtgcg
      121 aggtgttccg gttctactaa tgccccctat tcccccctta ataagggggt tggggggatt
      181 cttcagactt tcttgtctcc cacattgaga ggctgctctg tgtgactcta ctctcggaac
      241 gcaatacgag tgggcttact cccactcaat aactactttg ccacactctt ttgtttcaaa
      301 caaagccaag gcttcgtcag cgcggtcgat cggaaaccga tgggtaacca ttctgtcgag
      361 tttgagatct ttttcaacca gaaacttgat cagatcgaag accatccaga cagggagcac
      421 gaaagatccc tgaagggtga tctccttgcc gacaatgggt cccgcaggcc atccaggctc
      481 cccgcgaccg gcaacggtgc atatctttcc accacgcgcg caggtgtcca ctgcccattg
      541 ccgtgcctgc ggtgcggacg aggtctcgta gaccagagtc ggcgctttgc acgcttcgcg
      601 aattgctttg ggcacattct ccgtctgtgc aatgtctata acgacatctg ctccgaagag
      661 tgaagccagt tgcagtcgag gctgcctttt tccgacagca acgactctgg ctccaagggc
      721 tttccccatt gcgacgccac agagtccgac tggccccaag ccgagtacca gaagtgtgtc
      781 tcgcgtggaa ggctggagtt tgttcatcgc agaaaaactg gttccagcac cacaggcaat
      841 aagggctcca tcaacaaatg agagcgaatc cggcaacttc atggcattgc gctcggggac
      901 gaccatcaag tcagcagcgc ttccctgaat ggggccgccc agacctctgg tctgagcgca
      961 ccactgcatc tgtcctcggc gacactcccg acacttgcca caactgaaat agtggtagac
     1021 cgaaacgcga tcccccggct gaagagaggt cactcctgtt ccgatgtcca caacaacgcc
     1081 gcttggttca tggcatggta tacggtttcc catccgatcc agttggtcgg atgacatgtg
     1141 atagtagacc agatcactcc cgcaaatgcc ggagcaccgc attgcaacaa gaacttctcc
     1201 ttcaccggca accggatcag gaacttctcc aatatgactt ctcccatcgc catcgagaat
     1261 gagtgccttc aacgtgaact gccctcctgt gccaagtgca aaacgttctg aaaaagactt
     1321 ccagcgataa taacacaaaa ccagtatgag tttctcaatg caagttcaac caaacttgat
     1381 cagacactgg gacaaagttc agtgaacgca caaaagaatg cggaagaaga taactatggg
     1441 gggaaagcag aatttagaac tcgtctgttg gcgtgggtgt tctcgttacc gttcgggttg
     1501 gagtgaacgt tcgatcgggt gttcgtgtgg ctgaacgtgt tggtgtcggt gtttttgtag
     1561 gagccagagt cggtcggacg ctgcttcggt tcacggtaaa tgaggcatca tagtttcgac
     1621 catctttcga gccccacatg aacccttcaa acttgggcac atagcctgac gtgactgatg
     1681 cagggatatc ctgaacagcc caaatctgaa aagtcgtgaa ggatttcagt gtacctcgca
     1741 tcagaccgtt ctcgaactgc gcactgatcg tgagaggata ctgtgtgtca tctccggtgc
     1801 ccgtcacctg gctgccactg tgtttgagat gccacaggaa ctgacctgtc cggttgaccg
     1861 gatggtttgc cggatcggag aagtactcgg tgaagttgcc tacccagaag ccgtccacaa
     1921 cgtagtccgg caccggtgtt ggtgtgttgg ttggcatctc ggttggcgtt ggtgtggctg
     1981 atggcacggg cgtcgccaga ccatgccaca cggagatcag cagccggaag tcctgatgat
     2041 caatgacacc gttgccatct agatccgagt tctcgagtgg aggcatcgct tgcacggaag
     2101 cgggctgctg agcactggaa agaagggcca tgacgaatac aaacgccacc agcacaaaac
     2161 cgcccaagag ggctggtacg agtctttgaa catgggcgct tgtcttgctc atcatcactt
     2221 ttccagattc agttgaccgt agacggccgt gctttcggct ttccctcggt agacccctgt
     2281 caaggtggtc gagtttatca ggtgcagagt gaatgtcggg ttgaaatcca ttctcagtgt
     2341 accggtcatg tagaccgtct cattgagagt ccctgagaca gtcatctcac gtgtgccgga
     2401 cttgcctgtc ttcattcctt ctgcagtcac ggtgctacct gtcgaagtaa caatccagat
     2461 catcgagcct gaatctcgtt caaaaggctt gttcaaggga tcattgggtt cgtagaattc
     2521 cttgaaagag cctcgccaga taccaccgag attgggcatt actgacgttg gggttcgggt
     2581 cggtgttccc gttctcgttg gtgtgggtgt tcgagttgcc gtttgggtcg gcgttgggga
     2641 ctgctgattg gcatattccc acatcatgac aaacaaatcc ctgtcatcca caacgccgtc
     2701 gccattggca tccccgaagg gagtgggttt ggctgtgaaa gtgggtgttg cggtgagagt
     2761 cggcgtcctg gttggtgttt cagtcggggt gtagggtggt gtattggttg gcgatgaagt
     2821 cggtgtccaa gttggccctg ggggcgtcgg tgcagcgaca ttccacgtgg tcagatcgtg
     2881 tactgaatga ccaacttgtg tcatggcgct cagagagtaa ccggaactga acgtctgtcc
     2941 ggtgacacga atctggtagt ctgtgttggg cgtgggtacg aactcgcacc aaatccgacc
     3001 ggacggactt ccgacattcg actgcacttc ctcggttccc tgaagcacat gcatgaaggt
     3061 agactgacca gcagtttcct tcaggtagta catactgata ctgacaaaat ccacttcacc
     3121 gacaaagaga cgaaagaaat cgtcattgcg aagctgcagg ccaacatagc gtcctggagt
     3181 cacatccgcc ggtgtgttgg cgacgcgttc cagaaggtca tcctgtcctg cttccgtgcg
     3241 gatcggcacc acctgttcaa tccaaacagg gaagatcgga agactggttg atgcggacga
     3301 aatgttgaac tccactgcac ctgcttcagt ccccgtccgg tactcaaacc agaaaagacc
     3361 attggcgtct gtgtaccctg ctcttctgtt gatcacgcct cccaatgggg caggaggaac
     3421 cacgtccaga tcatagctga tattcttgag ctcttcacct ggatcgtatt cgctattctc
     3481 gttgatgtcg ctgaaaagcc gagttgagaa ctcacgggtc gcgccgtccc caattgtgac
     3541 agcaatggga cgataggcat caaccagatc cgccgctcgt acattagaca gaagcataca
     3601 aaaacaaacg cccgccagaa tcagaaagcc gtcccggcgt agcgtttgag cggaaacgaa
     3661 atatctcatt
//
LOCUS       DAOQUC010000221         3657 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_28767_length_3657_cov_3.035258, whole genome shotgun sequence.
ACCESSION   DAOQUC010000221 DAOQUC010000000
VERSION     DAOQUC010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3657)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3657)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3657
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28767_length_3657_cov_3.035258"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..820)
                     /locus_tag="PKH07_05065"
     CDS             complement(<1..820)
                     /locus_tag="PKH07_05065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34352.1"
                     /translation="METLKQEAAARLNNVRDIDPPVLLERANLVADWIIRNQITDRQD
                     ANKGRSIRSYDAKTKEKILTGNWMCGNIANTMCAMYKRTGQERYLASAELAAHYLMSL
                     QVMDRQSRYFGAIRELTPQSIEFAPRDAVGGAWGLVWLAETTGNSEYLHRAVLFADWL
                     IEKGMYQGWPMYAIYMDDSLADFYSRGHFHSGTGLFLYDLFRLTGDQRYIDKGLLPIA
                     TIYRDSFIRDDGFLVKEMDPFTGKVTDPKSEDEPLTYAINDDFGALMMIAASRLF"
     gene            complement(835..2310)
                     /locus_tag="PKH07_05070"
     CDS             complement(835..2310)
                     /locus_tag="PKH07_05070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34353.1"
                     /translation="MIFERERLLFFDWHVVESSENTQLTLNPPTKKGPCLFIDREWEL
                     DGARAYSVLEWNGEFRLYYEINPGGDRVCFALAASRDGINWEKPNLGAVSFNGSSSNN
                     IVDVQGEYVGGACVFVDPTGPDDHRFKLLGHNPHEGMFLLTSPDGLCFKRQTGCLLAH
                     NPDSLVSAFYDPRVGKYRVYIRAWNRERPIEQVPGSRTITMAELDTLFEPFPVDPNPP
                     DPWPQKPPKKVFGDTEVSVMRRLNREIVPYVIAPDEQDPPLADLYQSAAIHYMPEVYF
                     AFPTLYYHYPGPPEGFINDGILDIQFAASRDGRVWQRDLRGSYVRLDLPDGHCTKLLH
                     LITGIVPWKHIMSQYYVGGSRTHGQGRKPGVAKPKEPTNRSGKPFCHRLEQRLDGFVS
                     ADSAYMGGHLITKPFISGSRLLLNIDTSASGVASAALLDEQGKEIQGFTLDECDRIQG
                     NDTQYEVTWNHGADLSRIRGHAVRLHLRSRSTKLFAVTTTD"
     gene            complement(2430..>3657)
                     /locus_tag="PKH07_05075"
     CDS             complement(2430..>3657)
                     /locus_tag="PKH07_05075"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HNT34354.1"
                     /translation="GRDWTGAYASIGGHVTVVKDGRVPVDKWEGKDWKDHVHPRSCVA
                     CNDEYIFFVVVDGRSERSHGMTFEQLGNFCKDMLGATWAISQDGGGSSTLVVNDKVLN
                     VPSDGRERSVANGYVMVNVQPGAYSRAFSSGQSISLPAEAMLRLGPGTNYGVNANLDE
                     GTAVEIRNHPLNGVLAKGTHWWNVQSPMLEGWISEEQLSQKTSQESHSTFGTTVKFTD
                     SPPVLDGLLNEPVWQNAGRISGFLLAEGSLKPEDDTEVLLLYDEQALYLGFIAKESNI
                     AKVQAEQSGRDAFVWRDEAVEAFLDVGDASTAYFQVATNIKGSMFDLLEDRAGNLRFE
                     WNGNWEVRTSLQQASWTAEFRIPFSELGVTESPKGTVWKVNFTRCDYAGTQQNSAWGA
                     TVNFHNQEAFKEIHFE"
BASE COUNT          838 a          991 c          948 g          880 t
ORIGIN      
        1 cgaagagacg gcttgcggca atcatcatca acgcgccgaa gtcatcattg atcgcgtatg
       61 tcagaggttc atcttctgac ttgggatcag taaccttgcc ggtgaaagga tccatctcct
      121 tgaccagaaa gccatcgtct ctgataaacg aatcccggta gattgtcgcg atgggcagga
      181 gtcctttgtc aatatagcgt tgatccccag tcaaccggaa caaatcgtag agaaacagtc
      241 cagttccgga atgaaaatga ccacgcgagt agaaatcagc aagactgtca tccatgtaga
      301 tggcatacat aggccagccc tgatacattc ccttctcgat taaccagtcc gcaaaaagta
      361 ccgcacggtg gagatactcc gaattgcccg ttgtttcggc gagccagaca agtccccaag
      421 caccaccaac ggcgtctcta ggcgcgaact cgatggactg aggggttagt tcgcggattg
      481 ctccaaagta cctgctctgg cgatccatga cctgaagtga catcagatag tgggctgcca
      541 gttcggccga agccagatag cgctcttgcc cagtgcgttt gtacatggcg cacatggtgt
      601 tggcgatgtt gccgcacatc caattgccgg taagaatctt ctccttggtc ttggcatcat
      661 agctgcgaat cgatcgtccc ttgttggcat cctggcgatc cgtgatctga ttacgaataa
      721 tccagtctgc gacaaggttc gctctctcca acagcaccgg tggatcaatg tctcgaacgt
      781 tgttcaaacg tgcggctgct tcctgtttca gtgtttccat agggtccctc ctcctcagtc
      841 tgtagtggtc acggcaaata gtttcgtgct tctgcttctg aggtgcaacc gtactgcatg
      901 tccccgaatt ctcgaaagat cggctccatg gttccatgtc acctcgtact gtgtgtcatt
      961 gccctgaatc ctgtcgcatt catccaaggt gaatccttgg atttctttgc cctgttcatc
     1021 cagcaaggca gcgctggcaa cgccgctggc ggaagtgtct atgttgagca gcaaacggga
     1081 gccggagatg aacggctttg tgatcaggtg gccacccata taggcactgt ccgcagagac
     1141 aaagccgtca aggcgctgct ccaatctatg gcaaaatggt tttcccgaac ggttggtcgg
     1201 ttctttgggc tttgcaacgc caggctttcg gccttggcca tgtgttcgag aaccgccgac
     1261 atagtattgg ctcataatgt gtttccatgg gacgattccg gtgatgagat gcagcagctt
     1321 ggtgcagtgt ccgtctggaa ggtcaagtcg gacataagaa ccgcgcaaat ccctttgcca
     1381 gactctccca tcgcgactgg ctgcgaactg aatatcgaga atgccatcat tgataaaacc
     1441 ttcgggtggt ccagggtagt ggtaatacag cgttggaaat gcgaagtata cttccggcat
     1501 gtagtgaatt gccgcagact ggtagagatc ggcaagaggg ggatcctgct cgtcaggagc
     1561 aatcacgtag ggaacgatct ctcggttcaa gcggcgcatg accgaaactt cggtgtctcc
     1621 aaagactttc ttgggtggtt tctgcggcca gggatctggt gggttgggat caaccggaaa
     1681 gggttcaaaa agggtgtcaa gttcagccat cgtgatcgtt ctgctgccgg gtacctgttc
     1741 gatgggacgt tcgcgattcc acgctcgaat gtagacgcgg tactttccaa ccctcggatc
     1801 atagaatgcg gaaacaaggc tgtcggggtt gtgagcaaga agacagcccg tctgcctctt
     1861 gaaacaaagc ccatcgggag aagtgagcag gaacattcct tcatgaggat tgtgcccaag
     1921 gagcttgaat cggtgatcgt ctggaccggt tggatctaca aagacgcaag ccccgccaac
     1981 gtattctccc tgaacatcca caatgttgtt tgaagaggat ccgttgaaac tgacggcacc
     2041 gagattgggc ttctcccagt tgatcccatc tcgactggct gccagagcga agcatacacg
     2101 atccccacct gggttgattt catagtagag tcggaactca ccattccatt ccaagaccga
     2161 gtaggcacgc gcgccgtcca gttcccattc cctgtcaatg aacaagcagg gacccttctt
     2221 ggtgggagga ttcagagtca actgagtgtt ttcagaggac tcgacgacat gccagtcaaa
     2281 aaataaaagc ctctcgcgct caaaaatcat cgtttaccct ttcatgggaa gaggcttccc
     2341 tcgatacggg gaggcgattc acgccctgta acttgccttg gagtattcaa gttctcagcc
     2401 cccgctcaca tacagccatc cgcctacctc tactcaaaat ggatttcctt gaacgcctct
     2461 tgattgtgga agttgacagt ggccccccaa gcgctgttct gctgtgttcc agcatagtcg
     2521 caccgggtga aattaacctt ccagactgtg cccttgggtg attcggtcac tcccagttcg
     2581 ctgaacggaa ttcggaactc ggctgtccac gatgcttgct gaagcgatgt ccgaacttcc
     2641 cagttgccgt tccactcaaa acgaagattc cctgcgcgat cttccagaag atcgaacatg
     2701 gaacctttga tgttggttgc cacctgaaag taagccgttg aggcgtcccc gacatcgaga
     2761 aaagcttcga ccgcttcatc ccgccagaca aaggcatcac ggcctgactg ctcggcctgc
     2821 accttcgcga tgttcgattc cttggcgatg aatccaagat agagcgcctg ctcatcgtac
     2881 agaagaagaa cctccgtatc atcttctggt ttgagactgc cttcggcgag cagaaaaccg
     2941 gatatccgac ccgcattctg ccagacaggc tcattgagaa gaccgtccag cacgggtggg
     3001 gaatcggtga atttgacagt agtaccgaag gttgagtggc tctcctggct ggtcttctgt
     3061 gagagttgct cttcagaaat ccacccttca agcatgggcg actgtacatt ccaccaatgc
     3121 gtgcctttgg ccagaacgcc gtttaacggg tgattgcgga tttcgactgc cgtgccctca
     3181 tccaagttgg cgttgacacc atagttcgtt cccggaccga gtcgcaacat tgcctctgca
     3241 gggagtgaga tgctttgccc actggagaaa gctcttgagt aagcgccagg ctgtacattg
     3301 accatcacat aaccgttggc gactgatcgc tctcgtccgt ctgaaggaac attcagcacc
     3361 ttgtcattga cgacaagtgt tgaagaaccg ccaccatcct gcgaaatcgc ccaagtggct
     3421 ccgagcatgt ccttgcagaa gttgcccagt tgctcgaagg tcattccgtg acttcgttct
     3481 gagcgtccat ccacaactac gaagaagatg tactcgtcat tgcaggcgac acaactcctg
     3541 ggatgaacat gatctttcca gtctttgcct tcccatttgt caacgggcac tctgccgtct
     3601 ttgacgaccg tgacatgtcc accaatgctg gcataggctc cggtccagtc tcgcccg
//
LOCUS       DAOQUC010000222         3653 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_28807_length_3653_cov_3.551695, whole genome shotgun sequence.
ACCESSION   DAOQUC010000222 DAOQUC010000000
VERSION     DAOQUC010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3653
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28807_length_3653_cov_3.551695"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..795
                     /locus_tag="PKH07_05080"
     CDS             <1..795
                     /locus_tag="PKH07_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015050188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0046034 - ATP metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FliI/YscN family ATPase"
                     /protein_id="HNT34355.1"
                     /translation="LGMIARYTSASVNVIALVGERGREVREFIERDLGQEGLKRSVVV
                     VATSDTPALVRILAASVAITIAEYFRDRGLDVMFMMDSVTRFAMAQREVGLAVGEPPS
                     TRGYTPSVFALLPKFLERAGRTDHSGSITGLYTILVEGDDMNEPVADQVRSILDGHVV
                     LSRQLAEANHYPAIDVLNSVSRVMRDMVPPEHMKSSQTIREALAVYQRAQDLINIGAY
                     KEGNNPQIDWAMKLHGGIVKFLRQGFDEYLNYEQCVAQVRQLAGEK"
     gene            819..1271
                     /gene="fliJ"
                     /locus_tag="PKH07_05085"
     CDS             819..1271
                     /gene="fliJ"
                     /locus_tag="PKH07_05085"
                     /inference="COORDINATES: protein motif:HMM:TIGR02473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HNT34356.1"
                     /translation="MRRFQFSLEALLQVKQHQLDVLQAEIAELERRQNDRRVLLTRLG
                     EELENTAQSRMTSILAGIDADSLQEYFHRWVYLFECIQRLDREIHDSDIFLESRRDDY
                     RILYREHQMLEEMKSRARQQWWETVLKEEQKTLDEFGTLRFVRNTGSF"
     gene            1275..1973
                     /locus_tag="PKH07_05090"
     CDS             1275..1973
                     /locus_tag="PKH07_05090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34357.1"
                     /translation="MLKVFAILIVATGLLIGVLYLTGAIKPNTWELPFKKATIVEPVK
                     SEGTESAEDSAQQTPEPLYETKEVSDLIAGTSAEIWKGMTENLKARTQELEKLQQQLA
                     QQEAQQAKDRELLQKERLDLVALQTKMEGQIKELERIRAELDARMDEQASELVAQRIK
                     IVEKMEPTQAAEMLAGLEIAQAVSVLAGMKERTAGEIMTVMAQDQVMNATAQLILRDF
                     PYPIHLPIPLKAVK"
     gene            1981..>3653
                     /locus_tag="PKH07_05095"
     CDS             1981..>3653
                     /locus_tag="PKH07_05095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34358.1"
                     /translation="MLLEQGFLSLFTRSSGSVAEGLSVEPSRSAEAETFSPEFAALLL
                     KLLGQSQNGIQQGNLNLVQLITHEPSNQTSFPSLDSSNQSQGVVRSTAHTELFLEQRP
                     PMVQKPSANLAQYPSEIFTEDPNSVVSVFIPLRSSPPVTVQQGREHVRGILVRMNQTP
                     QKTDVNKNDPSPRQLKILGEVLIPVEDLHRVLPLGDTVISEKDTDKPESTETDSNTKT
                     VHHEHRLSLESDRSYRQEIDAGLERADRASTSNLQIGLTASSNISARDPLEILTIELR
                     EIQHDAIIVSNLEIQSADTLMGMLSGRDDLAEHPEARIAPKPALRVRAENSTGEDKTP
                     DRIQIPYAPRVAGDATTATVTALKADTGADTQTKTDAKPAIFLESTSRVEAFQTPDQN
                     ATEQATPRVLVSEGVLTDEASSAHNFSHATAEPVTDIRSVNQQEMLDFLALLRNLNAT
                     RLEVFVAPEHQEASPCLKTTLVSDSKAAAENGTLLEPTAVKSTPRQQAAPSPIEIKSS
                     IESRPHETQNQQVENARPDSRTTTDVPALRSMNAPTQTIAVHLQPTTPTV"
BASE COUNT          939 a          967 c          976 g          771 t
ORIGIN      
        1 ctcgggatga tcgcccgata tacgagtgcc agtgttaacg tcatcgctct tgtcggcgag
       61 agaggacgag aggttcggga gttcatcgag cgagatctcg gccaagaggg actcaagcga
      121 agcgttgtgg tcgtcgccac ttctgacacg cctgccttgg ttcggattct tgctgcatcg
      181 gttgccatca ctatcgcgga atactttcga gatcggggct tggatgtcat gttcatgatg
      241 gattccgtga ctcggtttgc gatggctcag agagaagtgg gtctggctgt tggagaaccc
      301 ccttcgacac ggggatatac gccatcggtc ttcgcgctcc tgccaaaatt cctggaacgc
      361 gcgggaagaa cagatcactc cggaagcatt accggtcttt acaccatcct ggtggaaggc
      421 gatgacatga atgagcccgt ggcagaccag gttcgatcta tcctggacgg tcatgtcgtt
      481 ttgagccgac agctggccga agccaatcac tacccagcca ttgacgtgct caacagcgtg
      541 agccgagtga tgcgtgacat ggttcccccc gagcacatga aatcatctca gacaattcga
      601 gaggcactgg cggtctacca aagggcgcag gatctcatta acattggagc atacaaagaa
      661 ggcaataacc cacaaattga ctgggcgatg aaactccatg gtgggattgt gaaattcctt
      721 cggcagggct ttgatgagta tttgaactac gagcagtgtg ttgcccaggt gaggcagttg
      781 gccggtgaaa aatagcccca acacggagaa aagaatctgt gagacggttt cagttcagtc
      841 ttgaggcatt gctgcaagtg aagcagcacc aactggatgt tctgcaggcc gagatcgctg
      901 agttggagcg ccgtcagaac gatcgtcgtg ttctcctcac gagactgggc gaggaattgg
      961 agaacactgc ccagtcgcga atgacctcaa tcctggctgg cattgatgcg gacagtctgc
     1021 aagaatactt tcacagatgg gtctatctgt ttgagtgcat acagaggctg gatcgggaga
     1081 tccacgactc ggatatcttt ttggagtctc gaagagacga ttaccggatc ctctatcggg
     1141 aacaccagat gctcgaagag atgaagtccc gtgcaagaca gcagtggtgg gagacggttc
     1201 tgaaagagga acagaagacc ctcgatgagt tcggcacgtt gcggtttgta cgcaacacag
     1261 ggagtttcta agtcatgctc aaagtctttg caatactgat tgtggcaacc ggtctgctga
     1321 ttggggtgct ttatctgaca ggtgccatca aaccgaacac ttgggagctt cctttcaaga
     1381 aggcgacaat cgtcgaaccg gtcaaatcag aaggcaccga atcagccgaa gacagcgcgc
     1441 agcagacgcc cgagcctctc tacgagacca aggaagtttc cgatttgatt gcgggaacca
     1501 gcgcggagat ctggaagggt atgactgaga atctcaaagc caggacacag gaactggaga
     1561 aactccagca acaactggcc caacaggaag cccaacaggc aaaggatcgg gaacttctcc
     1621 agaaggaacg tttggacttg gtagcgctgc aaacaaagat ggaaggtcag atcaaggaac
     1681 tcgagcgaat tcgagccgaa ctggatgcac gaatggacga acaggcttcg gaactggtcg
     1741 cccagagaat caagattgtt gaaaaaatgg agccgactca ggccgccgaa atgcttgctg
     1801 gcctggaaat tgctcaagca gtttctgtac tggcaggcat gaaggaacgt acggcaggtg
     1861 agattatgac ggtaatggct caggatcagg ttatgaatgc aacagcacaa ctgatcctca
     1921 gagactttcc gtatcccatt catctgccaa tacctttgaa ggcggttaag taatgagtcg
     1981 atgcttctag aacagggttt tctctccctt ttcacacgct cttcagggag cgtggcggaa
     2041 ggtctttcgg ttgaaccttc ccgttctgct gaggcggaaa ctttttcccc tgagtttgct
     2101 gctttgctgc tcaaactgct cggtcagagc caaaacggca ttcagcaggg caatctcaat
     2161 ctggttcagc tcatcactca cgagccatcg aaccagacct ctttcccatc gcttgattca
     2221 tccaatcagt cacaaggtgt cgtccgctcg acggcgcata cggagttgtt tctggagcaa
     2281 agaccaccga tggtgcagaa accatcggcc aacttagcgc agtatccctc tgagattttc
     2341 acagaagatc cgaactcagt tgtcagtgtt ttcatccccc tccgcagttc cccgcctgtg
     2401 actgtgcagc aaggacgcga acacgtccgc ggcatcctgg tgcggatgaa ccagacgccg
     2461 cagaagaccg atgtcaacaa gaatgatccg agtcctcggc agttaaagat tctgggggaa
     2521 gtcttgatcc ccgttgaaga cctgcatcgc gttctcccac tcggagatac ggtaatctcg
     2581 gaaaaggata ccgacaagcc cgaaagtact gaaacggact ccaatacaaa gacagtgcat
     2641 catgaacaca ggctgagtct tgagtccgat cgctcataca gacaggagat tgatgccggt
     2701 ttggaacgag ccgacagagc ctccacatcc aatttgcaga taggcttgac ggcctcatcc
     2761 aacatctcgg caagagaccc actggaaatc ctcacaattg aactccgaga gatccagcat
     2821 gacgcaatca tcgtttccaa ccttgagatt cagtcggccg acacgctcat gggtatgcta
     2881 tcaggaagag atgatctcgc agagcatccc gaagccagga ttgctcccaa gcctgctttg
     2941 agggttaggg cggaaaactc cacaggcgaa gacaagacgc ccgacaggat tcaaatcccc
     3001 tatgcgccga gagtggctgg tgatgccacc actgccaccg tgaccgcatt gaaggccgac
     3061 actggcgcgg atactcagac caaaacagac gccaagcccg cgatcttcct ggagtccaca
     3121 tctcgggtgg aagccttcca aactccagat cagaatgcca cggaacaggc taccccacgg
     3181 gtacttgttt cagaaggtgt tctgactgac gaagcgtcat cggctcacaa cttctctcac
     3241 gccacagcag agccggtcac tgacatacgt tccgtcaatc agcaggagat gctcgacttt
     3301 cttgctctgc tcaggaatct gaatgccacc cgccttgagg tcttcgttgc gcctgagcat
     3361 caagaggcct cgccttgttt gaagactact ctggtttcgg actcgaaggc cgctgccgaa
     3421 aacgggactc tgctcgaacc gaccgcggtc aaatcaaccc ctcgtcagca ggctgcaccg
     3481 tcgccgatcg aaatcaagtc ttcgatcgaa tctaggcctc acgagacgca gaatcagcaa
     3541 gtcgaaaatg caagaccaga ttccaggaca acaactgatg tgcctgcatt acgctccatg
     3601 aatgctccaa cgcagaccat tgcagttcat ctccaaccca caacgccaac ggt
//
LOCUS       DAOQUC010000223         3650 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_28841_length_3650_cov_3.539082, whole genome shotgun sequence.
ACCESSION   DAOQUC010000223 DAOQUC010000000
VERSION     DAOQUC010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3650
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28841_length_3650_cov_3.539082"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="PKH07_05100"
     CDS             <1..237
                     /locus_tag="PKH07_05100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34359.1"
                     /translation="VSSTILLFVNKVEPFTAITMKAFYYVIPHLELFDLSRKVVHVWQ
                     PVPAWVLFGLSAYAAVYVILPLTFASLIFSRKSL"
     gene            234..1301
                     /locus_tag="PKH07_05105"
     CDS             234..1301
                     /locus_tag="PKH07_05105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34360.1"
                     /translation="MKSSGLRVNLWQIAVAISVVALVATARLGSALLAYHNAEEARVS
                     DYSNPMQGGFAVHVARELRLSIADLFWLKTEEYIHDGVQHRPHGEGDEFATTHQEQSE
                     HVHTEECDHTHEDEEYDAEGIPLKQDDSSVVGLAHDCEDHGGTLIPSRQSDFRGFLGD
                     LERQTQPYSPEHHLSADPQELLPWLRLATLSNPHHIRAYRGIAFFLATYLKRPAQAIQ
                     VIQDALVLNTDHPQLLLVLGRLHFYERKDPVAASKYFERVIELWKEDSNKGTWDEEDQ
                     FAVRQSFVHLAYCEMRQGNKDGAIWWCEEGMREFPDAEAVKRYRMRLLGQVPPRRNPP
                     RDQIPREEDHLHQHDAGRTDH"
     gene            1332..3398
                     /locus_tag="PKH07_05110"
     CDS             1332..3398
                     /locus_tag="PKH07_05110"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HNT34361.1"
                     /translation="MNRALPLLLLFGLFLFGVAVRRVHAVENPVLPSVADSGVMKHRG
                     EYYLMGVGTNGHIYISDDLIQWKGTYPAMTMKNAWARGGAATDWQIHACDLNYVNGVF
                     HLYWSVNYLDADLDIRQIGHSVAVEPLGPYIEPVTETWFDGRIDPQLFVDDDGAPYFY
                     KVLFEHGNVIYGERLKDPWTHGDDPKRLLSTLEGTWEQLDPQGYLVNEAPWVFKYRNK
                     YYMLYGANHVGLGHYAIGCAQADSPMGFSNATKYPHAVLDSKIISVGSDLSGSKQEFR
                     IHNCGQPSVVRGPNGFEWWVVYFAKYNESASSQAVDRVLFFDRELFIDGPSTTNTPGY
                     HPNPSLPTFRDLFCEDQQQSLAERWQLACGQWVSCDGLLQQQATAGGAMAIVKSTPAR
                     NYLFEASIKLSTGKGTAGVIAYWHDSENWLSLCLDRKNQGWFAERMEQGSLTLERHEL
                     PSTFNFDVFHTIRVCHNADTFEVQIDGIGAGDVWTVRTSFDKTGLPALCTKDTSALFD
                     GVTYTIGWDEFDSTIRGWGDALCGHEAAGNWNVTERGLTQNDRDSHACVYKGDLVDEY
                     EFMTQVSFAEAAESDTYAAAGIYAVYVDASNWLSAVVERPSQDLVIRGQILGDPVREI
                     RVPKSLEDSFNLRTIKMRDKVILFVDGVLGATVETAFPASQVGLLTENCEVVFNGITC
                     FDKSKR"
BASE COUNT          859 a          909 c         1017 g          865 t
ORIGIN      
        1 gtcagcagta cgattctgct tttcgtcaac aaagtcgaac cgttcaccgc gatcacgatg
       61 aaggccttct attatgtcat cccgcacctg gaattgttcg atctgtccag gaaggttgtc
      121 catgtgtggc agcctgtccc tgcctgggtg ctgttcgggc tttcagccta cgccgctgtc
      181 tatgtcattt tgcccctgac cttcgcgagc cttatctttt caaggaaatc tctatgaaat
      241 cctcagggct gcgtgtgaat ttgtggcaga ttgctgtggc gatcagtgtg gtggcactcg
      301 ttgccactgc tcgtctcgga tcggcacttc ttgcgtatca caacgctgag gaagccagag
      361 tctcagacta cagcaatcca atgcagggtg gctttgccgt tcatgtggct cgcgaactca
      421 gactgtccat cgcggatttg ttctggctca agactgaaga gtatattcac gatggagttc
      481 agcaccgtcc gcatggagag ggtgacgagt tcgcaaccac ccatcaggaa cagtcggagc
      541 atgttcacac cgaagagtgt gaccacaccc atgaagatga ggaatatgac gcagaaggca
      601 ttcctctaaa acaagacgat tcgagtgttg ttgggcttgc ccacgactgc gaagatcatg
      661 gcgggacctt gatccccagc aggcagtctg attttcgcgg tttcctgggt gatctggaaa
      721 ggcagaccca accatactca ccagaacacc atctgagcgc tgatcctcag gaactgcttc
      781 cctggctcag gcttgcaaca ctctccaatc ctcatcacat cagagcctat cgaggtatcg
      841 ccttcttcct cgcgacttat ctgaaacgcc ctgcacaggc cattcaggtg atccaggacg
      901 cgctggtgct caataccgat catccgcaac tgcttttggt cttgggacga ctgcacttct
      961 acgaacgcaa ggatcccgtt gcggcgagca agtattttga gcgcgtgata gaactgtgga
     1021 aagaggattc gaataaggga acctgggatg aagaagatca gttcgctgtc agacagagtt
     1081 ttgtgcactt ggcctactgc gagatgagac aggggaacaa ggatggggca atctggtggt
     1141 gcgaagaagg aatgcgggag ttcccagatg ccgaggcggt caagagatac cgaatgaggc
     1201 tgttggggca agttccaccg cggagaaatc cgcctcgaga tcagataccg cgtgaagaag
     1261 atcacttgca ccaacatgat gcgggaagaa ccgatcactg agagtttcac tatccaaagg
     1321 agaattcact ggtgaacaga gcgttacctc ttctgctgct gtttgggctg ttcttgtttg
     1381 gagttgcagt tcgcagggtg catgcggtcg aaaaccccgt tcttccctcc gtggcggaca
     1441 gcggcgtgat gaaacatcgt ggcgagtact acctgatggg agtgggcacc aacggacata
     1501 tctacatttc cgacgacctc attcaatgga aaggtaccta tccagcaatg acgatgaaaa
     1561 atgcctgggc gcgaggtggg gctgcaactg actggcagat tcatgcctgc gatctcaact
     1621 atgtcaacgg cgtttttcac ctttactggt ccgtgaacta tcttgacgcc gatctcgaca
     1681 tccgacagat cggtcattcc gttgctgttg agccgttggg tccgtatatc gagccagtca
     1741 ctgagacatg gtttgatggg cgcattgatc cgcagctgtt cgtggatgac gatggcgccc
     1801 cctatttcta caaagtgctc ttcgagcatg ggaatgttat ctacggagaa cgtctgaagg
     1861 atccgtggac acatggggac gatccgaaac ggttgctctc gacattggaa ggcacctggg
     1921 aacaactgga tccgcaaggc tacctggtca atgaagctcc ctgggtcttc aagtaccgaa
     1981 acaaatacta catgctctat ggtgcaaacc atgtggggct tggacattat gcaatcggtt
     2041 gtgcacaagc cgattctccg atgggtttct cgaatgcaac caaatatccc catgcggttc
     2101 tggattcgaa gatcatctct gtcggcagtg atttgagcgg ctccaagcaa gagtttcgca
     2161 tccacaactg cggacaacct tctgttgttc gaggtccgaa cggcttcgag tggtgggttg
     2221 tctatttcgc gaagtacaac gaaagtgcat caagccaggc ggtggacagg gtgttgtttt
     2281 ttgatagaga actattcatt gacgggcctt ctaccacgaa cacgccaggc taccatccca
     2341 atccgagcct tcccacattt cgtgacttgt tctgcgaaga ccaacagcag tctctggcgg
     2401 agcggtggca acttgcctgt ggacaatggg tatcatgcga cggtttactg caacaacagg
     2461 cgaccgcagg tggagccatg gcgatcgtca aaagcacacc tgcccggaac tatctcttcg
     2521 aggcaagcat caaactcagc acgggtaaag gtacggcggg agtgatcgcc tattggcatg
     2581 acagcgaaaa ctggctctcc ctctgtctgg acaggaaaaa ccagggctgg tttgcagaga
     2641 gaatggaaca aggcagcctc acactggaac gtcatgagtt gccgtccacc ttcaacttcg
     2701 atgtctttca tacgattcgt gtctgccaca atgcagacac cttcgaagtc cagattgacg
     2761 gaatcggcgc aggggatgtc tggacggtta gaacatcatt cgacaaaacc ggtctccccg
     2821 ctctttgcac aaaagacacc tctgcactct ttgatggtgt cacctacaca attggatggg
     2881 acgagtttga cagcacaatc cgaggctggg gagatgccct ctgcggtcat gaagcagcgg
     2941 ggaattggaa tgtcaccgaa cgggggctta ctcagaacga cagggactct cacgcgtgtg
     3001 tctacaaagg ggatttggtg gatgaatatg agttcatgac tcaggtttct ttcgccgaag
     3061 cagctgagag tgacacatac gctgcggcgg ggatttatgc cgtttatgtg gatgcttcaa
     3121 actggttgag tgcggtcgtc gagagaccca gtcaagacct tgtgatcagg ggacagattc
     3181 ttggagatcc ggtgcgggag attcgcgtcc ccaaaagcct ggaagactct ttcaacctgc
     3241 gtaccatcaa gatgcgcgac aaggtcatac tgtttgtgga tggagttctt ggtgcaacgg
     3301 tagaaactgc gtttccggcg tctcaggttg gattgctgac agagaattgt gaagtcgtgt
     3361 tcaatggcat cacatgtttc gataagtcga aacgctgagt ttgcatcggg cgcggacgca
     3421 tgaggcgacc aacgttactc acgagaggcc atacggcatg cactcatttc cttcttgcca
     3481 aaagggcgtg atggtgacag attacactcc tatgagacca cccgccagag cggtggtttt
     3541 ctgatttgag caggtgcgcg ggagagtggg ctccagaggc tcacagacgc tggcctgctc
     3601 atcaagaact cgagttgaat tttgtttggg aggttgcagg atgcttatcg
//
LOCUS       DAOQUC010000224         3636 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_28995_length_3636_cov_3.059481, whole genome shotgun sequence.
ACCESSION   DAOQUC010000224 DAOQUC010000000
VERSION     DAOQUC010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3636
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28995_length_3636_cov_3.059481"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            16..3630
                     /locus_tag="PKH07_05115"
     CDS             16..3630
                     /locus_tag="PKH07_05115"
                     /inference="COORDINATES: protein motif:HMM:NF021126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HNT34362.1"
                     /translation="MAEIIWGAMLAALHIIPFLDVLSATERVGRLPYLFLTSGSLQWR
                     QLVTFLLPNRFGTIIDGTWSLSPDPAAYMESCGYCGLLPLVLLPGLIRRRTDSQVVFF
                     ACLGLVGLVLSLGSSTPLYRVVAAVPLLNQARIPARWLSLVMLSVAVLGALGMQSLLS
                     QTHDRPVRRHFLSLVFVATLLLLLGAPLVTDKGLLMANIHAPIALLLTASAVVVCSCN
                     NRLPVVWKSVVLFAVLLLDISVWGGRLNPVVPSAIVFDPPPLSKQITSPDFSRTAHLP
                     PSQPPADSPLPIETGWKDGLDKYRNTMASLPGNSSLLYDVAVVGALSGTQLRDTKAIL
                     GMPAVSGGSLFFNRDASSPLLELAGVNYYINNSPVAEKHWKRLTDHYPYLFARSHAES
                     KAFLVHDSECIPDEVVQTAYLSSGKRDFRQTALLFEESPLVSSGVTPSASEQVTIAEY
                     SDKRVVVNVTTQWQSMLVLSDQYFPGWRAYVNGVETRIFKADGAFRSVMVEPGSHRVE
                     FVFDPSWFRSASLLTLVGIALCVILLILSQKAKFFAAFTPQITKKHLIWFGLLFLVLL
                     YAVREPSDSDLWWHMASGRYIVNEGRVPQNDVLSFSTDSAPWIYYSWLFGVLAYAVTL
                     LSAGHSLGSDLLVLQAAQIILCLAIFLSILLFHRKEHFWSASALLALAIVASSVRWIL
                     RPHLFGQLYLVLFYVVCDSYHRHGKKALFALPLVMILWVNMHGTYVLAPCVVGAFLVA
                     TLLGRDGSSSVRKAFVFGCLLVAVCLLVGLNPYGFSVLRVPLELASSAELRDKIGEWG
                     PPLRFDVMGPQGVLYLIAIGAFIHNLVQKRFASAFVLLAPLALYPVARRHVDVLAVLV
                     PLCAAEPFGIWCRFLFARAPKWGFVLAAALMLLTVSRGIFLLREIYGRPSIQVRYPFA
                     ALDFAVRNGLHLKYFSKYEWAGQISWLDHPRSKHFVDARFRVFNQTLRGDYEEIIHAT
                     PDAMRLLDQYGCEAVLLPYRPGGLVDPLPSLLFDSSEWCLVYWDDLAVLFVRKGLLER
                     ERKTDIQISHANPVWLQIPSGLSDEERYLVEKDLLCVIERLPSCQHARLLLADCFRTQ
                     GHASDGIQILQSLCSEPGVSFTCFAQLGAALLSEGRTTEAVIFFTEATHRFPREPAAF
                     LNLAQLLMASGDLSGARRAAQNSLNLAPDDQSALQVLASIEERLGNVAESMRIRQRLD
                     "
BASE COUNT          679 a          979 c          956 g         1022 t
ORIGIN      
        1 cgtcggcggg atggcatggc cgaaataatt tggggtgcca tgcttgcagc tttgcacatc
       61 atcccatttc tggatgttct gagcgctacc gaacgggtgg gaagactgcc ttatcttttt
      121 cttaccagcg gctcccttca gtggcgtcag ttggtgacct ttcttctgcc aaatcgcttc
      181 ggaacaatca ttgatggtac ttggtcgctt tcacctgatc cggcagcata catggagagt
      241 tgtggatatt gtggtttgct gccactggtt cttttgcctg gactcatacg cagaagaaca
      301 gactctcaag tggtgttttt cgcctgtctc ggacttgttg ggctcgtttt gtcgctgggg
      361 tcaagcactc cgttatatcg agttgtggcg gccgttcctc ttttgaatca ggcgagaata
      421 cccgctcgat ggctgtccct ggtcatgctg tcagttgctg ttctgggggc tttggggatg
      481 cagtcgctgc tgtctcaaac acacgacagg ccagttagac gccacttcct tagtcttgtc
      541 tttgtcgcca ctcttttgct gttgctgggg gctccccttg tcacggacaa gggtttgttg
      601 atggccaata tccacgcccc catcgctctg ctgttgaccg cttcagctgt tgttgtctgc
      661 tcttgcaaca accgactgcc ggtcgtctgg aaatctgttg tgttgttcgc tgtgctgttg
      721 ttggatattt cggtatgggg tggcaggctg aatccggtcg tgcctagtgc aatcgtcttt
      781 gatccgccgc cactgtccaa gcaaatcact tcacccgact tctctcgcac agcgcatctc
      841 cctccgtctc agccccccgc ggattctcct ctgcccattg aaaccggatg gaaagatggt
      901 ttggataaat acagaaacac catggcttca ctgccgggca attcatcgct actgtatgac
      961 gttgcagttg ttggggctct cagtggaaca cagttgaggg acaccaaggc tattctcggt
     1021 atgcccgcag tcagtggagg cagtcttttc ttcaaccggg acgcatcttc accgctgctg
     1081 gaactggctg gcgttaacta ctacatcaac aactcccccg tggctgaaaa gcactggaaa
     1141 aggctgaccg atcactaccc ctatctgttc gccagaagtc acgctgagag caaggccttt
     1201 ctggttcatg acagcgagtg cattcccgac gaagtcgttc agaccgctta tctttcatcg
     1261 gggaaaagag acttccggca aacagcgctg ctctttgagg agagtcctct ggtttcatcg
     1321 ggagtgaccc cttcggcgag cgagcaggta acaatagcag agtattcaga caaacgcgtt
     1381 gttgtgaacg tcaccaccca atggcagtca atgcttgttc tcagcgacca gtattttcca
     1441 ggttggcggg cgtatgtgaa cggtgttgag accaggatat tcaaagccga tggggcgttt
     1501 cgttcggtga tggttgagcc aggttctcac cgcgtcgaat tcgtgttcga tccgtcgtgg
     1561 ttcagatcgg catcgttgct gacacttgtg ggaattgccc tgtgtgtcat tctcttgatt
     1621 ctctcgcaga aggccaaatt cttcgcagcc ttcactccac agatcacgaa gaagcatctc
     1681 atctggtttg gactgctgtt cttggtgctt ctgtatgcag ttcgggaacc ttccgattcc
     1741 gatctctggt ggcacatggc atcgggaagg tacatcgtca atgaaggacg cgttccccaa
     1801 aatgacgtct tgtccttctc caccgacagc gccccctgga tttactacag ttggcttttt
     1861 ggtgttctcg cctatgcggt cacactgctg tccgcaggtc actctctggg gagcgatctc
     1921 ctggtcttgc aggctgccca gatcattctt tgtctggcga tcttccttag catccttttg
     1981 tttcaccgca aagaacattt ctggtcagca tccgctctgt tggcattggc aatagtggct
     2041 tcatctgttc gatggatcct ccggcctcat ttgtttggtc agctgtacct ggttttgttc
     2101 tacgtcgtct gtgacagcta ccacagacac ggcaagaaag ccctatttgc gcttcctctt
     2161 gtcatgatcc tatgggtcaa catgcatggt acctatgttc ttgccccttg tgtcgttggg
     2221 gcttttctgg tcgcaacact gctcggtcgc gatggctctt catcggttcg caaagcgttc
     2281 gtgttcggtt gtcttctcgt tgccgtctgc ctgctcgtcg ggctgaatcc gtacggcttt
     2341 tccgtgctgc gggtgccact tgagttggcc tcaagtgctg aactgcgaga caagattggg
     2401 gaatgggggc cccctcttcg attcgatgtt atgggcccac agggtgtgct gtatctcatc
     2461 gccattggag ccttcataca caacttggtt cagaagcgat ttgccagtgc cttcgtactt
     2521 cttgccccgc tggctttgta ccccgtcgcg cggcgtcatg tagacgttct tgccgttctg
     2581 gttccactgt gtgctgcaga acctttcggt atctggtgcc ggttcctctt tgcacgcgct
     2641 cccaagtggg gttttgtctt ggcagcagca ctaatgctac tgactgtctc gcgcggcatc
     2701 tttcttctca gggaaatcta cgggagaccc tcgattcagg tcaggtatcc tttcgccgca
     2761 ctggatttcg ccgtacgcaa tggccttcat cttaaatact tcagcaagta cgaatgggcg
     2821 gggcagatca gttggcttga ccatccccgt tcaaaacatt ttgtcgacgc tcgattccga
     2881 gttttcaacc agacccttcg aggtgactac gaggagatca ttcacgctac cccagacgca
     2941 atgaggctcc ttgaccaata tggttgcgaa gccgtcctgt tgccctaccg acctggtggt
     3001 ctcgtggatc ctttgcctag tctgctgttt gattccagcg aatggtgtct tgtttactgg
     3061 gatgacctcg ctgtgctttt tgtccgaaaa ggtcttcttg aaagggagag aaagacggat
     3121 atccaaatat cgcatgcgaa tcccgtctgg ctgcagattc caagcgggct atcggatgaa
     3181 gagagatatc ttgtggaaaa ggatctcctg tgcgtcattg aaaggttgcc atcgtgtcag
     3241 catgctcggc tgcttctcgc agactgcttt cgcacgcaag gacacgcgag cgatggcata
     3301 caaatacttc agtccctgtg ctcggagcct ggtgtctctt tcacttgctt tgcccagttg
     3361 ggggcagcac tgctttccga aggcagaaca acagaagcgg tgatcttctt tacagaagca
     3421 acacaccgtt ttcctagaga accggcggcc tttctgaatc tcgcccagtt gctgatggcc
     3481 tctggcgacc tgagtggagc gcggcgtgca gcgcaaaatt cactcaacct ggctcccgat
     3541 gatcaatcgg cgctccaagt tctggcctcc atcgaagagc gtctcgggaa tgttgcagaa
     3601 tccatgagaa tcagacaacg actcgactga tgtttc
//
LOCUS       DAOQUC010000225         3634 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_29020_length_3634_cov_3.188041, whole genome shotgun sequence.
ACCESSION   DAOQUC010000225 DAOQUC010000000
VERSION     DAOQUC010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3634)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3634)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3634
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29020_length_3634_cov_3.188041"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /locus_tag="PKH07_05120"
     CDS             complement(<1..304)
                     /locus_tag="PKH07_05120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34363.1"
                     /translation="MKMLLAKPEGQFNTTVQVTGLLVIALFALGILLPGALEASIAPQ
                     VRLDAQWLSAQQSRYVGSEGHDNALQQLLNHLERIQGITLWTHDYPLLIPHVWDAHL"
     gene            complement(316..2313)
                     /locus_tag="PKH07_05125"
     CDS             complement(316..2313)
                     /locus_tag="PKH07_05125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34364.1"
                     /translation="MNLKSDTGKNRTRFFPAILIGLLASLTIWVVTPYTNFVIGSAYI
                     ADSFLPIIGLFLILLIVLFLNPLLRVLRPKCSFASRELAVIVGIIMMACVVPSQGLLR
                     MLPYALAAGPIHVRENARYSETIKEMNLPPSLFPDKMEFGADTPVSDYFATELPPGES
                     IPWGAWMPTIFVWGLMLMSTYLMMTGLALIVYPQWRRNERLSFPLLALHQSLIDDPDE
                     GNAFAPLFRQRSFWIAASLVLLLHIFAGWKLHNPEAVPAIPLNWNISKLFTEGLFRYL
                     PGHIRVNRIYFIFVGIAFFMPSRIGFSVWFFVVAYAAYSMIGSAYFPPYYGTTVVEHR
                     FGGMIALCLAVLWLGRAHWVHVFRCLFRGASTEEDRKDRNALIMFLIGCLGMVAWLIW
                     VGVQPVWALVFLGVGFMVSLIITRFVAETGIPFIRIDMGYQMGFLRLFPRAWLSPATL
                     FFSYFIVMFFPMASRVSCATLASHAIGLNEDQPPQTQVRYSWLIIALLAVGLFLCWNV
                     HLVVNYHFSASLDGIDRPVSAWGYNLISNFFTSVQELKEGRYASPIYNQGFHLAFGFT
                     LASVLQWASLTMPKWPIHPIGLLMVNTFYSNEAWFSVFLGWLIKVIILRYGGARLYRS
                     ARPVFLGLIMGEVFAGAFWSLVPVILVFLGRPYMRVQIQPY"
     gene            2490..3395
                     /locus_tag="PKH07_05130"
     CDS             2490..3395
                     /locus_tag="PKH07_05130"
                     /inference="COORDINATES: protein motif:HMM:NF015971.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 130 protein"
                     /protein_id="HNT34365.1"
                     /translation="MNKELLTRYANNPILTALDVPFLCNAVYNPGAVKFGNRYIMIPR
                     VEDGRRDNRLHVAESGDGIHFTIRPEPIMLPLDSEAQIWEKHMYDPRVTFLEGVYYIA
                     YCAQTMEETVRIGLMKTVDFESFERVGLITPPWNRNCALFPERIGGLYARFERPMNGA
                     DAVNFISYSPDLLHWGQAKAVELKTETWLRNKWGVGPSPIKTDEGWLVIFHGVWWAIG
                     PVYRLGVMLIDLEQPHKVIGQCPEFILTPREPYERIGEVNNCVFSNGAIVEPDGQLKV
                     YYGAADTCICLATGNLVELIAACKQ"
     gene            3449..>3634
                     /locus_tag="PKH07_05135"
     CDS             3449..>3634
                     /locus_tag="PKH07_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005935887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 1"
                     /protein_id="HNT34366.1"
                     /translation="MRVLLLTKAYPPWVGGVECHVQQLAEGLASKGVDTVVLACSPSR
                     QLERICKGNLEVILVPRL"
BASE COUNT          967 a          904 c          993 g          770 t
ORIGIN      
        1 ccaaatgcgc atcccaaaca tggggaatca acaagggata gtcatgcgtc cagagagtga
       61 ttccttggat tcgttccaga tgattgagca gctgctgaag tgcattatca tggccttccg
      121 atcccacata acgtgactgc tgagcagaga gccattgggc atccagacgc acttgcggag
      181 cgatggacgc ttcgagtgca ccgggaagaa ggataccgag cgcaaacagc gcgatcacca
      241 gaagtcctgt gacttggacc gtggtgttga actgcccttc aggctttgcc aaaagcatct
      301 tcatgaaata ctccgtcaat agggctgaat ctggacgcgc atataaggtc ttccaaggaa
      361 gaccaaaatg acaggaacca gactccagaa agcacccgca aaaacctctc ccatgatcag
      421 accgagaaaa acggggcgcg cagaccggta gagccgggct cccccatatc gaaggatgat
      481 caccttaatc aaccagccca gaaacacgct gaaccacgct tcgttcgagt agaaggtgtt
      541 gaccatcaag aggccgatag gatggattgg ccattttggc atggtcagac ttgcccactg
      601 caagacggaa gccagcgtga agccaaaagc caaatgaaag ccctgattgt aaatgggaga
      661 agcgtagcgt ccttccttga gctcctgaac agacgtgaag aagttgctga tgagattgta
      721 accccaggcg ctcacaggtc ggtcaatgcc gtccaggctt gcactgaaat gatagttgac
      781 cacaaggtga acattccagc aaagaaacaa gcccacggcg agcagggcga tgatcagcca
      841 ggaataccga acttgggttt gcggcggttg atcctcgtta agtccgatag cgtgcgatgc
      901 cagagttgcg cagctcacac gagatgccat ggggaagaac atgactatga aataggaaaa
      961 aaacagagtc gccggactga gccaggctcg gggaaagagt ctcagaaagc ccatctggta
     1021 gcccatgtca atccgaatga aaggaattcc ggtctccgct acgaaacgag tgatgatcag
     1081 agaaaccatg aatccgaccc cgagaaagac caaagcccaa acgggctgca caccaaccca
     1141 gatgagccat gcgaccatcc caagacaacc gatcagaaac atgatcagag cgtttctgtc
     1201 ctttcgatct tcctcagtcg aggcacctcg gaagaggcat cgaaagacat gcacccagtg
     1261 agctcttccg agccagagga cggcaagaca cagggcgatc attccaccga agcgatgttc
     1321 cacaacagta gttccgtagt acggcggaaa gtaggcggag ccgatcatgc tgtaagcggc
     1381 gtaggccacc acaaaaaacc aaactgaaaa tccaatgcgg cttggcatga agaaggcaat
     1441 gccaacaaag atgaagtaaa tccgattaac tcgaatgtgt cctggcaggt aacgaaacaa
     1501 accttcggtg aagagtttgc tgatgttcca gttaagtgga attgccggta ccgcttcagg
     1561 attgtgcaat ttccaccctg cgaagatatg aaggagaaga accagcgaag cggcgatcca
     1621 gaaactcctc tgccgaaaca gaggagcaaa ggcattgccc tcgtcggggt cgtcaatcag
     1681 cgattggtgc aatgcgagca agggaaaaga gagcctttcg ttgcgtcgcc attggggata
     1741 aacaatcagc gccagccccg tcatcataag gtaggtggac atcaacatga gcccccacac
     1801 gaagatggtc ggcatccatg ctccccaagg tatggactca ccgggcggga gctctgtggc
     1861 gaaatagtct gagaccggag tgtctgctcc gaactccatc ttgtcgggaa agaggctcgg
     1921 aggaaggttc atctctttga tcgtctcaga gtagcgggca ttctcgcgaa catgaatggg
     1981 gccagcagcc agagcgtatg gaagcattct cagaagtccc tggctcggta ccacacaggc
     2041 catcatgatg atacccacga ttacggcaag ttcccgactc gcgaatgaac actttggcct
     2101 caaaacgcgc agaagcggat tcaggaagag gacaatcagc agaatgagga aaagcccgat
     2161 gataggcagg aaactgtccg caatgtaggc actgccaatg acaaaattag tgtaaggcgt
     2221 gacaacccat attgtcaggg acgccaacaa cccaatcagg atcgctggga aaaagcgggt
     2281 cctattcttg cccgtgtctg atttcagatt cacaatgtgc tgttttcctt agtgcccttt
     2341 gagggtctac agccgtcttt caagttgtca aaagatagcc aaaatggggt tgctaaacaa
     2401 ggtctttctt cagtcgagac gagaacggtt gccggttgga tgtgcatccc acttgacagg
     2461 cgtctactcc tttccataat accaccacta tgaacaaaga actgctgact cgatatgcga
     2521 acaatccgat tctgaccgcg ttggatgtgc cttttctgtg caatgcggtc tacaaccctg
     2581 gtgcggttaa gttcgggaac agatacatca tgattccgag ggttgaagat gggcgccgag
     2641 acaatcggtt gcacgttgcc gagagcggcg acggcattca tttcacgatt cgaccggagc
     2701 cgatcatgct tccactcgat tctgaagcgc agatctggga aaagcacatg tacgatcccc
     2761 gagtgacctt tctggaaggc gtctattaca ttgcctactg cgctcagacg atggaagaga
     2821 ccgttcggat cggtctgatg aagacggttg actttgagag tttcgagcgc gttgggttga
     2881 tcacaccgcc gtggaaccgc aactgtgcct tgtttcccga gagaatcgga ggtctgtatg
     2941 cgcgtttcga gcgaccgatg aatggggcag acgcggtcaa tttcatcagt tattcccccg
     3001 acctgctgca ctgggggcag gcgaaggcag tggaactcaa gacggagaca tggcttcgaa
     3061 acaaatgggg tgttggtcca tcaccgatca agacagatga aggttggctt gttatctttc
     3121 acggagtctg gtgggcaata ggtccggtgt atcgtctggg tgtgatgctg attgatcttg
     3181 agcagcctca caaagtcatt ggtcagtgtc cggagttcat cctgacgcca cgagagccct
     3241 atgagcgcat cggtgaggtc aacaactgcg tattctccaa cggtgcaatt gttgagcctg
     3301 acggacaatt gaaggtctat tacggtgccg cggatacgtg catctgtctg gcgactggca
     3361 atcttgttga gctgatcgcg gcatgtaagc aatgatccga atctgtatgt tcttctctca
     3421 cgcaatgctt ttgtgttagg cttccttcat gcgtgttctc cttttgacaa aggcatatcc
     3481 accctgggtt ggaggtgttg aatgtcatgt tcaacagctc gctgaaggtc tggcgtccaa
     3541 aggcgttgat acagtagttc tcgcgtgcag tccgagccgt caacttgagc gcatctgcaa
     3601 ggggaatctt gaggtgattc tcgttccacg tctt
//
LOCUS       DAOQUC010000226         3626 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_29109_length_3626_cov_4.025203, whole genome shotgun sequence.
ACCESSION   DAOQUC010000226 DAOQUC010000000
VERSION     DAOQUC010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3626
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29109_length_3626_cov_4.025203"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1114)
                     /locus_tag="PKH07_05140"
     CDS             complement(<1..1114)
                     /locus_tag="PKH07_05140"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HNT34367.1"
                     /translation="MLFFKSKKKKTCVLGLDGVPFTLLQRFVADGTMPNAARIFSQGR
                     LQQMKVTLPEISAVSWPSFMTGANPGTHGIFGFTDLKPGSYAVRYPNYRDVKAMVLWD
                     KLENKRKRSIVLNQPGTYPAQPMNGVLVSGFVAIDLRKAVTPLKYKSDLEKMGYRIDI
                     NTAACRDDHALCIKELNETLAARKKAVEFFLQAEEWDYLQVVITGTDRLHHYLWDALD
                     NTEHPYHAAFLDFYRQVDAFIGWLYQKFQGESGRDDAADGFFMLSDHGFTGIRQEVYL
                     TRWLHNEGYLKFDVEQPESLEQMSAESRAFVMDPGRIMIHRSDRFPNGCVAAQDVEPL
                     KQEMTAKLLALECSGEKVIQAVHRAEDIYSGPYVSEG"
     gene            complement(1384..1986)
                     /gene="cysC"
                     /locus_tag="PKH07_05145"
     CDS             complement(1384..1986)
                     /gene="cysC"
                     /locus_tag="PKH07_05145"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HNT34368.1"
                     /translation="MKGFTLWFTGLSGAGKSTLAEQVEQILLERGMKVEILDGDVVRT
                     NLSKGLGFSKEDRDINIRRIGFVCHLLTRNDVVAISAAISPYKSVRNENRKLIGRFVE
                     VYVKCPIEVLSERDVKGLYKKALKGEIKNFTGVDDPYEEPDNPEIIVDSSKETVQESI
                     AKIIRTLELMNYIPASDSENEYSAEEEEEIKQRLKALGYI"
     gene            complement(2061..3440)
                     /locus_tag="PKH07_05150"
     CDS             complement(2061..3440)
                     /locus_tag="PKH07_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007044584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HNT34369.1"
                     /translation="MSKLVIIGLDCATPQLVFDAWLDRLPNIRSLVQRGIAGPLKSTL
                     PPITVPAWTAMMTSKDPGMLGFYGFRNRKSYDYEDLFFANAKYVKDKTVWNYLSRNRL
                     KSLVFGVPQTYPPKPLNGVLVASFLTPSKDVQFTYPDEIKKDLDEAADGDYIIDVKDF
                     RTDNKDKLVAAIYEMTRRRFKAFRKFYTSDSYDFAMMVEMGVDRIHHGFWRYFDKSHR
                     LYEPGNPYENVIEDYYKYLDEEIGKTLEILEPDTSVMLVSDHGAKTMTGAICINEWLQ
                     QKGWLTLKEKPTKPSRMELDMIDWSKTKAWGEGGYYSRLFLNVQGREPQGQIPQAEYE
                     QFRDEVKAALEGIVDEQGNNIGTKAFRPEEVYRQATGIPPDLIVYLGNLDWRSAGSVG
                     VGSIYMYENDTGPDDANHAEYGIFVFAPGAGRTVAPKDDYSIYDIAPTILKYFDVTVP
                     EDMIGTSIL"
     gene            complement(3443..>3626)
                     /locus_tag="PKH07_05155"
     CDS             complement(3443..>3626)
                     /locus_tag="PKH07_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34370.1"
                     /translation="TKTCPHDADQHVHLSGTKVREMLSRAELPPVEFTRPEVAEVLIK
                     HARQKVDGKAQEKKEK"
BASE COUNT          808 a          992 c          901 g          925 t
ORIGIN      
        1 gcccttcgga tacataggga cccgagtaga tgtcttcagc ccgatgcaca gcctgaatga
       61 ctttttctcc cgagcattcc aacgcgagca atttcgccgt catttcctgt ttcaacggtt
      121 ccacatcctg cgctgcaacg catccattgg gaaatcggtc tgagcgatga atcatgatcc
      181 ggcctggatc catgacaaaa gctctggact cggcgctcat ctgctcgaga gactccggct
      241 gttccacatc aaatttcaga tatccctcgt tgtggagcca tcgggtgaga taaacttcct
      301 gccgaatgcc tgtgaatccg tgatcagaca gcatgaagaa gccatccgcg gcatcatcac
      361 gtcccgattc gccctgaaac ttctgataga gccaaccgat aaaagcatct acctgacgat
      421 agaaatcgag aaaggccgcg tggtaaggat gctccgtgtt gtcaagggca tcccaaagat
      481 agtggtgcag tcggtccgtt ccagtgatga ccacttggag ataatcccac tcctcggcct
      541 gaaggaagaa ctcgaccgcc tttttgcgcg ctgccagtgt ttcgttgagt tccttgatgc
      601 atagagcatg gtcatcgcga caggcagcgg tgttgatgtc aattcgatag cccatcttct
      661 ccagatcgga tttgtacttc aacggagtga cggctttgcg caaatctatg gcgacaaaac
      721 cggacaccag aacgccattc attggctgcg cggggtatgt ccccggctga ttcagcacga
      781 tggaacgctt gcgcttgttc tccagtttgt cccagagaac catggctttc acatcacgat
      841 agttgggata tcgaacagcg taggagcctg gcttcagatc ggtgaacccg aaaatgccat
      901 gtgttcctgg attggctccc gtcatgaagc tcggccagct cacagcagag atctcgggca
      961 gagtcacttt catctgctga agtctgcctt gggagaaaat ccgagccgca ttcggcatgg
     1021 tgccgtctgc aacaaaacgc tgaagcagag tgaagggcac tccgtccagt ccaagaacac
     1081 aggttttctt tttcttgctt ttgaagaaaa gcacgctcta agaccctcct cttggcacgc
     1141 tgaccccttc ggctccttct gagaaacggg aagtctcaat ccctctctca tacaaggaag
     1201 gtttggggca aggcactttc ggaaaccact cccttctgtt gatgaccgta tcggctcagt
     1261 ttcggagtga gagatgctat gctctcccga aaaagggtgc tcagagtgca cccttcatgt
     1321 tgtcttgtat gaagtgtgaa ctctgaacag gatcgcagcg ggagccttct atgggcatcc
     1381 gtcctagatg tacccgagcg ccttcagtct ctgcttgatc tcttcctctt cctcggcgct
     1441 gtattcgttt tccgaatccg aggcgggaat gtagttcatc aattccagtg ttctgatgat
     1501 ctttgcgatg ctttcctgaa cagtttcttt gctggaatca acgatgatct caggattgtc
     1561 cggctcctca tagggatcgt ccacgccggt gaagttcttg atctcaccct tcagcgcttt
     1621 cttatacaac cccttcacgt cacgttcgga aagcacttca atcggacact tgacgtagac
     1681 ctcaacaaaa cgcccgatca gttttctgtt ttcattgcgt acagacttgt acggggagat
     1741 cgctgcggag atagcgacaa cgtcgtttcg tgtcaataga tggcagacga acccgatgcg
     1801 ccgaatgttg atgtcacgat cttcctttga gaagcccaaa cctttgctga ggttggttcg
     1861 caccacatca ccatcgagga tctcgacctt catgccgcgt tcgagcagaa tctgctcaac
     1921 ctgttccgcc aaggtggact ttccggcacc tgaaagtcct gtgaaccaca aagtgaaccc
     1981 tttcattcaa actgtcctcc aagaatttga tttccaaaag catctatcga aatcgggctg
     2041 ctctgcgccc gagccgtgca tcagagaatg gacgttccga tcatgtcttc cggaacggtc
     2101 acatcgaaat acttcaatat cgtcggcgcg atatcgtaaa tcgagtagtc gtcttttggt
     2161 gcaaccgttc ttcctgcgcc cggtgcgaac acgaatatgc catactctgc gtggttggcg
     2221 tcatcgggac cggtgtcgtt ctcatacata taaattgaac ccacaccaac ggaacctgcc
     2281 gagcgccagt cgagattccc aaggtacaca atcaagtcag ggggaatgcc cgttgcctgc
     2341 cgatagactt cttcgggtct gaacgccttg gtaccgatgt tgtttccctg ctcatcaaca
     2401 atgccttcaa gggcggcctt gacttcgtcg cggaattgct cgtactcggc ctggggaatc
     2461 tggccttgtg gttcgcgacc ttgaacattc aggaacagac ggctgtaata acccccttca
     2521 ccccaggctt tggtcttgct ccagtctatc atgtcgagtt ccatgcgaga gggtttcgtc
     2581 ggtttctctt tcagtgtcag ccagcccttt tgctgaagcc actcgttgat gcagatcgcg
     2641 cccgtcatgg tcttggcccc gtggtcggat accaacatca cggatgtgtc cggctcaaga
     2701 atctcgagcg tcttaccgat ttcctcatcg agatacttgt agtagtcctc gatgacgttt
     2761 tcatagggat tgccaggctc atagagtctg tggctcttgt caaagtaacg ccagaagcca
     2821 tgatgaattc ggtcaactcc catctcgacc atcatggcga aatcataact gtccgaagtg
     2881 tagaacttcc gaaacgcttt gaaacgtctt cgggtcatct cgtagatggc agcaaccagt
     2941 ttgtccttgt tgtcggtgcg gaagtccttg acgtcaataa tgtagtcgcc gtcagcagcc
     3001 tcatcgagat cctttttgat ctcatccggg tacgtgaact gaacgtcctt gctcggcgtc
     3061 agaaaactgg ctacaagcac accgttcaga ggcttgggcg gataggtctg tggcacgcca
     3121 aacaccaggg atttcagacg gtttctggat agatagttcc aaaccgtctt gtccttaacg
     3181 tacttggcat tggcgaaaaa caggtcttcg tagtcatagc tctttcgatt ccgaaaacca
     3241 taaaacccca acatacccgg atcctttgat gtcatcattg ctgtccaggc aggaacagta
     3301 attgggggca gtgtggactt cagtggtccc gctattccac gttgcaccag gctgcgaatg
     3361 ttgggcagac gatccagcca ggcatcaaag accaactgcg gagtggcgca gtccagtccg
     3421 atgatcacca gtttggacat ggtcacttct ccttcttttc ctgcgccttg ccatccacct
     3481 tctgacgcgc atgctttatc agtacctcgg cgacctcagg tcgggtgaac tccactggag
     3541 gcaattcggc acgggagagc atttcgcgga ctttggtacc gctcagatga acatgctgat
     3601 cggcgtcatg cgggcaggtc ttggtg
//
LOCUS       DAOQUC010000227         3626 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_29113_length_3626_cov_3.622515, whole genome shotgun sequence.
ACCESSION   DAOQUC010000227 DAOQUC010000000
VERSION     DAOQUC010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3626
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29113_length_3626_cov_3.622515"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            552..>3626
                     /locus_tag="PKH07_05160"
     CDS             552..>3626
                     /locus_tag="PKH07_05160"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HNT34371.1"
                     /translation="MIIHSRFFKSVCWFVVLAFIGTTSNLECLVFADNFLNSAKPQAV
                     GPGFRLQEQLEKLTQIGVGATLGKTLGESDLRQLSSNLARVGDDILALDEEIQAEFRE
                     NITKLSSPELPPVGLERYRAFIKEYEKRKGEVLSALAAAKQSKTPQEAQDNCAALNTV
                     VAKYSRRNAIDPAQVEVMPFRGRTIKDGQTENKSSADLEKDYKEFSYEKALASSSAPT
                     AEDLAETEDIVFHSEIIEAATRLEKNPIRMFRFVADNCYYQPYYGSMKGSLETFRQQA
                     GNDLDSASLLMALYRYSGIPCRYVSGEIEIPMAQALNYIGVENSRVMGSVLATNGVPA
                     VTMINQDTGEQFLRMKHYWVMAWLPSKYRGVGGGNSGSWIYLDPSFKQHVFTTGIDTT
                     EVLELDEQSQASQVESNIVHNSPYSFNFSMDGLRGDMNGYQTKMSNHFEANVPEEAPL
                     QQMLNYMDIVREDFDFLPGSLPYRVLSTAGNFSVVPANMRFYVTLNMKNYDPYDVFGD
                     GSSLSYRASTPFLAGKRIIVGYVPASSYDASIVAAYGGINNCPASAANLKPGVRINGV
                     LAKEGSGSRIGEEEDFEIAVSGPDGTNDRISNKVVVGSYHAVALDLQGVAPKQAYERL
                     EILQATVDRIKAMVDDASGSGQPTITVDRDDVAGEFLSVAGMVYWTHMMNYAEHGAMQ
                     ENILYVKGVSEAVVSFGLDTEYFFGMPRGVDPSGSGIDVDLNIFTPFNRKGDETVNTD
                     FAKRYGYRSSIEEHSMFDELLKEVFGTTTGAAPKVTTNPFLRETEAENYPTVSAVKAL
                     KIANDQGIPIYSLDSMDAYNAVSGELSLFSNVRSDIVNGLRAGKTVVVHQRAINFYDW
                     HGFGYIITDQATGDATYMISSGSNGGDTAEKSKIEGAIEWLFGPGSTGYVIATGLFVI
                     SQFGEFLGKACGIIGAVWAIVTLIIELKNAEADGKYCPQAKEMLIDVLIMFSALSALL
                     SIIGIFVGPLMAFVLFIIGMYLSIAIDILKVMLPIICAYIDALDSVRLQIQRY"
BASE COUNT          860 a          905 c          980 g          881 t
ORIGIN      
        1 cctacacgcc aaccaacacc cgaacaccga cgcgaactcc gactcgcacc ccgacctgga
       61 ccccaacgcc aactctgaca ccgacaccga cggaatccgc gacggaaaca ccaacaccat
      121 gagcgggtca gttaagggcg ggcgatcttc ttttcaagag cagccctcag aaccgcttcg
      181 cgaacctgaa gggctggcac ccagaggatt caaccggaac gtgagaaaag agagggcacc
      241 cggctgtatc acctgcatca ctgagccagt acagaaattc gccatttctc cttgacggaa
      301 ctacgtcttt ggggctatcc tacgtgcaac tggaatctgg attttccgaa catgccagca
      361 tgttcggatc tggtcagtta gacgcggtaa gctaatacga tggttcacga aaattcagtg
      421 aggggattct gctattcagg aaaggcctgc cagcagtgta tccgcttgtc agaacagcct
      481 gatatcgttt ctcttttttc gaaccttcaa ggcgatctgc attgccttaa tcctttcagg
      541 agtgtgaaga gatgataatt cacagtcgct ttttcaagag tgtttgttgg tttgtagtgc
      601 tggcctttat cgggacaacc tcgaacctgg agtgccttgt ttttgctgac aattttctaa
      661 attctgccaa acctcaagcg gttggcccgg gctttcgcct ccaagaacag cttgagaaac
      721 tcactcagat tggcgtgggt gcgacgcttg gcaagactct tggcgaaagc gatctacgcc
      781 agttgtcctc caatctggct cgcgtgggtg acgacattct ggccttggat gaagagatcc
      841 aagctgagtt cagagaaaac attacaaagc tttccagtcc cgaacttccc cctgttggtt
      901 tggagcggta tcgggctttc atcaaagagt atgagaagcg aaagggagag gttctgtcgg
      961 ctctggctgc tgcaaaacag agcaagaccc ctcaggaagc gcaggacaat tgtgcagcgc
     1021 tcaatacggt cgtcgcaaag tattcgagaa gaaacgccat tgatccagcg caagtggaag
     1081 tgatgccctt cagaggcaga acgatcaagg atggacagac cgagaacaag tccagtgcag
     1141 atctggagaa agattacaaa gagttctctt acgagaaggc tttggcgagt tcttctgctc
     1201 cgacggctga ggatctcgct gaaacggaag acatagtctt ccacagtgag atcattgagg
     1261 cggcgacgcg acttgagaag aatccgattc gaatgtttcg atttgttgcc gacaactgct
     1321 actaccagcc ttactacggg agcatgaaag gctctttgga gactttccgt cagcaggcgg
     1381 gcaacgacct tgattcggcg tcgctgttga tggctctgta ccgctactcg gggattccgt
     1441 gccgatacgt ctccggagaa attgaaatcc cgatggctca ggcgctgaac tacattggag
     1501 ttgagaactc acgagtgatg ggttctgtgc ttgccaccaa tggcgttcct gcagtgacga
     1561 tgatcaacca agacacaggt gagcagtttc tcaggatgaa gcactactgg gtcatggcgt
     1621 ggttgccttc gaaatatcgt ggagtgggag gaggcaacag tggatcctgg atataccttg
     1681 atccttcttt caagcagcat gttttcacga cgggtattga caccaccgaa gtccttgaac
     1741 tggacgagca gagccaggca agtcaagtcg aatccaatat cgtccacaac agcccttact
     1801 ctttcaactt cagcatggac ggtcttcgag gcgacatgaa tggatatcag accaagatgt
     1861 ccaatcattt cgaggcaaat gttcctgaag aagcgccgct tcagcagatg ctgaactata
     1921 tggacattgt tcgggaggac tttgacttcc ttcctgggag tcttccctat cgggttttga
     1981 gcacggcggg aaacttctcc gtggttccgg ccaatatgcg cttctacgtt accctgaaca
     2041 tgaagaatta tgatccatac gacgtcttcg gggatggttc cagcctttcc tatcgagcca
     2101 gtacgccttt tcttgcaggc aagcgtatca tcgtcggtta cgttcctgct tcttcttacg
     2161 atgcctcaat cgtcgctgcc tacgggggca tcaacaactg ccccgcctcc gctgcaaacc
     2221 tgaaacccgg tgttcgtatc aacggggtac tcgccaagga aggatccggc tcccgaatcg
     2281 gggaggaaga ggattttgag attgcggtga gcggtccgga cggcaccaat gatcggatat
     2341 ccaacaaggt tgtcgtgggt tcctatcacg cggtcgcctt ggatttgcag ggtgtagcac
     2401 ccaaacaagc ctatgaacga ctggagattc tccaggccac agttgaccgg atcaaggcga
     2461 tggttgacga cgcatcgggg agtggccagc cgacgatcac ggtcgatcga gacgacgtgg
     2521 ccggcgagtt tttgtctgtt gccggtatgg tctactggac gcacatgatg aactacgccg
     2581 agcacggagc gatgcaggag aacattctct acgtgaaggg tgtctctgag gccgttgtct
     2641 ctttcggctt ggacacggag tactttttcg gcatgcccag gggagttgat ccgtccggct
     2701 caggcattga tgttgatctg aacatcttca ccccgtttaa tcgcaaaggt gacgagaccg
     2761 tcaataccga ttttgccaag cgatacgggt accgttcttc catcgaagag cacagtatgt
     2821 tcgatgagtt gctcaaggaa gtttttggca caactacggg tgcggctccc aaggtgacga
     2881 caaatccctt cctgcgtgag acggaagctg agaactaccc aaccgtctcg gcggtcaagg
     2941 ctttgaagat cgccaatgat cagggcattc cgatctacag tttggattcg atggatgcct
     3001 acaatgccgt ctctggtgaa ctcagtttgt tttccaatgt gcgctcggac attgtcaatg
     3061 gtttgagagc gggcaagacg gttgttgttc atcaaagggc aatcaacttc tatgactggc
     3121 atggatttgg gtacatcatt accgaccagg caaccggtga cgcaacgtac atgatctcct
     3181 caggttccaa cggtggagat acggcggaaa agagcaaaat cgaaggggcg atcgaatggc
     3241 tctttggacc aggttctacc ggctatgtga ttgcgactgg attgtttgtc atctctcagt
     3301 ttggcgaatt ccttgggaaa gcctgcggca tcatcggtgc cgtttgggcg attgtcaccc
     3361 tgatcatcga gttgaagaat gctgaagccg acggcaagta ttgccctcag gcaaaggaga
     3421 tgctgattga tgttctcatc atgttcagcg ctttgagcgc gctgttgagc atcataggca
     3481 tattcgttgg cccgttgatg gcatttgtcc tgttcatcat cgggatgtat ctcagcatcg
     3541 ccatagatat tctgaaggtc atgctaccaa tcatctgtgc gtatattgac gctctcgata
     3601 gtgtccggtt gcagattcaa cggtat
//
LOCUS       DAOQUC010000228         3626 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_29115_length_3626_cov_3.270232, whole genome shotgun sequence.
ACCESSION   DAOQUC010000228 DAOQUC010000000
VERSION     DAOQUC010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3626
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29115_length_3626_cov_3.270232"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1053)
                     /locus_tag="PKH07_05165"
     CDS             complement(<1..1053)
                     /locus_tag="PKH07_05165"
                     /inference="COORDINATES: protein motif:HMM:NF024807.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HNT34372.1"
                     /translation="MSRRICVFLAVLWTLSLVSVAQAVNWSVLPSNDVGRCGHTSVYD
                     DALDRMIVFAGRGSSGYLNSCTQMSLSLGNEAWKSLAPSGESLPVPRAYHTAIVYPAD
                     SGNYMVIFGGKGGTTRVNYYSDTWAMFMKKGEEAWSQLQPKGIIPPARESHSAIYDPK
                     WKRMIVFGGRNAATSMNDLWVLDLATLTWSAHGSTQKGPSNRAGHSAVYDAKNERMLV
                     FGGVLDTGLYTTEIWSLDLSIEDGGWTKLPTEGKGAHGVAWHCAVYDDPNKRMLTFAG
                     TDGAQTSYLQCVSYVSSVTGSWMALSPTGVTPPARQDVAAVLDPEDERLVVFGGQAGF
                     VGIFGDAHELSWRPLPT"
     gene            complement(1363..2556)
                     /locus_tag="PKH07_05170"
     CDS             complement(1363..2556)
                     /locus_tag="PKH07_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNT34373.1"
                     /translation="MTSRERVLTTLNHKEPDRIPIDFGAMRSTGIMAVEYNRLKAHLG
                     HHQGETFVYDLMQQLAEPEPWILKLFSADVEQLHRLRPAFGYSILNKRAGVLPDGSPC
                     IYPADFQPQPYKEGEALFAYGQPFAYRPESSYVFYAMVHPLSGARDTGEIDQFTFPTI
                     DDDEFQYLSWRSKQLRESTDRAILGAFGGNVIESGQFDFGYDEFLMLMATEPLLVHHY
                     FDRLTEHWIQCLDRYIEAVGDNIDIIQVGDDLGTQHAPVISPKMYREMVKPYHQRIYQ
                     HIKAKSKYHVFLHTCGAISELIPDLIEAGVDILNPVQTSAEGMAPESLKRSFGNDVVF
                     WGGGCETQTTLFQGTPQDVKREAKERLGIFGAGGGYVFTQIHNILVGVPPENVVALYE
                     AAQEC"
     gene            complement(2599..>3626)
                     /locus_tag="PKH07_05175"
     CDS             complement(2599..>3626)
                     /locus_tag="PKH07_05175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34374.1"
                     /translation="SAEPELQLETLFKPPKQGFLGADGALSIPLTATKSVWIFGDTFV
                     GEPSASIRRGSTFIRNSVAVLDRSSNPLGEVTFYWDRHSDKPSSFFPHDGIHWHWAGT
                     GTMVDGMLFLFCYKLEPATNFFQFRIVDLCLIRVENPLDCPEQWAFEITDLGFGNLDK
                     GYCSAAYAADGFIYLLGFDHLLMKSRTVLARVSVEALKSHPSRQCFEFWAHGKSGSFW
                     SAEEHNLESILSEGVAESALQRVGDLFVYTTFTPFDPAIRLYFAQDIRGPWEGPIHVY
                     DVPDHQKAKTVFSYAAKPHPELSSDPGEIILTYAANCSDFDRVLDDLELYYPRFVRVR
                     LRSTPLG"
BASE COUNT          916 a          961 c          928 g          821 t
ORIGIN      
        1 tgtcggcaaa ggacgccagc tcaattcatg ggcatctccg aaaatcccaa caaaaccagc
       61 ctgccctccg aaaacaacca gtcgctcgtc ctcaggatcc aagaccgccg ccacatcctg
      121 ccgagctggg ggagtgacac cagttgggct taaggccatc caggaacccg ttaccgagga
      181 gacataagag acgcattgca gataactcgt ctgtgcccca tccgtacccg cgaaagtgag
      241 cattcgtttg ttgggatcat catacacagc gcagtgccag gcaaccccgt gggctccttt
      301 tccttccgta ggtaacttcg tccatccacc atcttcgatg gacaagtcca gcgaccagat
      361 ttcagtcgtg tagagacccg tgtcgagaac tccaccaaag acaagcatgc gttcgttttt
      421 tgcatcgtag accgcactgt gtccggctcg atttgagggt cctttctggg ttgaaccgtg
      481 ggcgctccag gtaagagttg ccaagtccag cacccagagg tcattcatgg acgttgccgc
      541 gtttctgccg ccaaaaacaa tcatgcgctt ccatttggga tcgtagatgg cactgtggct
      601 ctctctggca ggtgggataa ttcctttggg ttggagttga ctccaggctt cttcaccctt
      661 cttcatgaac attgcccagg tgtcggaata gtagttgacc ctagtggttc ctcctttgcc
      721 accaaagatt accatgtagt tgccagagtc tgccggataa acgatggctg tgtgataggc
      781 tcttggcacg ggaagagact cccctgacgg cgccagggac ttccatgctt cgttgcccag
      841 gctcaggctc atctgtgtgc agctgttcag ataaccactg gaacctcgac ctgcaaagac
      901 gatcatgcga tccaacgcat cgtcatagac cgatgtgtga ccacaccgtc caacgtcgtt
      961 ggagggcaga actgaccaat tcacagcctg agccacagat accaggctca acgtccacaa
     1021 gacagccaga aatacacaga ttctcctaga catgaccaga ctcctttcga cggatcgacc
     1081 gtcaaaaaac cgaatgccta acgataggga ttccctgcaa acagacgcga aaaaccgact
     1141 ccgtcggatc tgacggaata ctgttgaaag gatgaacacg atccatgccc ctcacgatcc
     1201 ctcagccgct ctcgagatcg atcctgaaag gaaagaccat acagagatga aagcaaactg
     1261 agaccagaaa cctcccaatc tatttccgca atcataatag ccgcaatagg gcaaaggcgt
     1321 caagaagaaa tggcagatat gaaccgggta ttgtcgttgc catcagcatt cctgggctgc
     1381 ttcgtacaga gcaaccacat tctctggagg gactcccaca aggatattgt ggatttgcgt
     1441 aaagacgtag ccgccaccag cgccaaagat accaagacgc tccttggcct ctcgcttcac
     1501 atcctgtgga gtcccctgaa aaagtgttgt ctgcgtctcg cacccgccac cccaaaagac
     1561 aacgtcattt ccaaaactcc gcttgaggct ctcaggagcc atcccctcgg ctgaggtctg
     1621 aacagggttc agaatatcaa cccctgcttc aatgagatcg gggattagtt ctgagatggc
     1681 tccgcaggtg tgtagaaaca cgtggtactt gctctttgcc ttgatgtgct ggtagatcct
     1741 ctgatgatag ggcttcacca tctcgcgata catcttcggg gagatgacag gtgcgtgctg
     1801 ggttccaaga tcatcaccga cctggatgat gtctatgttg tctccaaccg cttcaatgta
     1861 gcgatcaaga cactgaatcc aatgttctgt caggcggtca aagtaatgat gaaccaacag
     1921 gggttcagtt gccattaaca tgagaaactc gtcatatcca aaatcgaatt gaccggattc
     1981 gatgacattg ccgccaaaag cacctagtat ggcacggtca gtgctctccc tgagttgctt
     2041 tgagcgccag gagaggtact gaaactcgtc atcatcaatc gttggaaatg tgaactggtc
     2101 aatttctcca gtgtctctgg ctccgctgag aggatgcacc atggcataaa agacgtagct
     2161 ggattctggg cgataggcaa aaggttgtcc gtatgcaaac aacgcttctc cttccttata
     2221 gggttgaggc tggaaatctg ctggatagat acaaggacta ccgtctggca aaacgcccgc
     2281 tcgcttgttg agaatggaat acccaaacgc cggacgcagt ctgtggagtt gctccacgtc
     2341 tgccgagaag agcttcagta tccatggttc gggctccgcg agttgctgca ttaggtcgta
     2401 gacaaaggtc tcgccttgat ggtgaccgag gtgtgcctta aggcgattgt attccactgc
     2461 cataattcca gtagagcgca tggctccaaa gtctatcgga atccgatctg gttccttgtg
     2521 attcagtgtt gtcaagactc tctctctgga tgtcattgtc aaacctcctt gccgaacgaa
     2581 gcagtctctt tatcaacctc agcccaaagg cgtgcttctt aaccgaacgc ggacgaatct
     2641 cggatagtac agttccagat catcaaggac tctgtcgaag tcggagcagt ttgctgcata
     2701 ggtcagaatg atctcgccag ggtcactgga taactctgga tggggttttg cagcgtagct
     2761 gaacacggtc tttgccttct gatggtcggg aacgtcgtaa acgtggatag gcccttccca
     2821 gggtcccctg atatcctgag caaagtagag tctgatggct ggatcaaagg gagtgaaggt
     2881 tgtgtagaca aaaagatccc caacgcgttg cagtgccgat tcggcgacgc cctcactgag
     2941 aatactctcc agattatgct cctctgcaga ccagaatgag ccggacttgc catgggccca
     3001 gaactcaaag cactgacgcg aagggtgtga tttcagagcc tcgacagaga ctcgtgccag
     3061 aaccgttctg ctcttcatga gaaggtggtc aaagcccagc agatatatga agccatccgc
     3121 agcgtaggca gcactgcagt agcccttgtc caggtttcca aaaccgagat cggttatttc
     3181 gaaagcccat tgttcagggc agtcgagggg attctcgact cgaatcagac agagatcaac
     3241 gatgcggaac tggaagaaat tcgttgccgg ctccaatttg taacagaaga gaaagagcat
     3301 accatcaacc atggtgcccg tccctgccca gtgccaatga atgccgtcat gggggaagaa
     3361 tgaggacggt ttgtcagaat ggcgatccca gtagaaagtg acttctccaa gagggttgga
     3421 tgaacgatcc agaaccgcaa cagagtttct gataaaagtg cttccgcgac gtatgctcgc
     3481 ggacggttct ccgacaaagg tatctccaaa aatccacact gacttcgtgg cagtgagcgg
     3541 gatggacaag gcaccgtcgg caccgagaaa cccctgtttg ggtggtttga aaagggtttc
     3601 cagctgcaac tcaggttctg cagaag
//
LOCUS       DAOQUC010000229         3623 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_29146_length_3623_cov_2.621076, whole genome shotgun sequence.
ACCESSION   DAOQUC010000229 DAOQUC010000000
VERSION     DAOQUC010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3623
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29146_length_3623_cov_2.621076"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..370
                     /locus_tag="PKH07_05180"
     CDS             <1..370
                     /locus_tag="PKH07_05180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34375.1"
                     /translation="LDALSLPAQPGVFGISLARSDSMQTDRVLFASSYRDGDKMSALS
                     GHLKLIHNIKSGKREYYDLQKDPKETQPLDEPDRPEFQNLDHALEKWQEINAAQQPLS
                     ASTELTNELSEELKSLGYLR"
     gene            419..2356
                     /locus_tag="PKH07_05185"
     CDS             419..2356
                     /locus_tag="PKH07_05185"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNT34376.1"
                     /translation="MKLREHLLSEQWGYWVATVIGAVALLIGLIVSRTDVGKVLELRL
                     LDARFLWRGRAEPESSIVVLGIDEESIDFFRDKGKPWPWPNRIYAEAVQSLTDMGASV
                     IVFDKIFKDPTSGSSDEDEQLLAQSAKRHGKTIYAVDMDKREVGGIIRESVNEPLAVI
                     AESGILGFIDLPMDEDGAVRRVTFLASCQGKQYLNLDLQAYLLIKGKTQDNVHLTPKK
                     NLQVGSLAIPLDFRNSAYINYPGKSVFEEYAFFQLFDPEQKALLQELEIFRGKTVFIG
                     VTSELFHDDYVIPFSRVSKARSTSETEREFAGTTREYGVHIHAAVLHTLLKGPRIQPV
                     PLKMVGPISTALGLLVVLLLSRAGFKLSLLLSVGLAFCCMVAGFWLFLHHAVYMNLVS
                     PLSAIAFPSVAMLYYKFLYEQSQKSQIRSYWSRYVSDDIVDLMVKGKISVELGGQRRR
                     VTVLMSDIRDFTPLCERLSPSDLIAQLNEYFSEMARAIFAHGGTIDKFIGDAIMAYWG
                     FPHEQDDHPQRACLAAVEMLRHAELLCKKWEDEGKPTFRMGVGINTGDVVIGNVGAEV
                     DNETIQNFTLLGDAVNLAARLESENKNHGTTLIVSEYTWEQVRDLKGFRARSLGELKV
                     KGKSQALQCYSIELDETVQTG"
     gene            2399..3355
                     /locus_tag="PKH07_05190"
     CDS             2399..3355
                     /locus_tag="PKH07_05190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34377.1"
                     /translation="MRSILIPTLLVLLICVGVSAHAQGQAVGDAKITSVSGTVIVRSA
                     SSSKLTPAKPAMVVSVGDTVMTRKASEATLLFQKTGAFKKLGSDENYVVQVEQQDSAT
                     KSGLGDVLSGLTELFTSQSDTREELLAISATVRKATDESGVVKNNYIFRMPLNTDLLE
                     ETPTIRWNPIQDARAYSVSIADPTGVFLEKSTMETALDLAALNKPLARNVTYTVKVRA
                     SVPDKGIVEDSGYIRVKSVQEVSQIKQAEQMIHDETGKGLDETSSALILGRYLESSRL
                     YQPAYQLYEQMLKKDSSADFAKLRMEIIEVTCQVKEPTVESN"
     gene            3374..>3623
                     /locus_tag="PKH07_05195"
     CDS             3374..>3623
                     /locus_tag="PKH07_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012098279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HNT34378.1"
                     /translation="MQQGSLSNAEIRERLGFSDDQLMRECELQPYQASGPGGQKRNRT
                     YSAVRLQHHPTGISVTAAESRSQLDNRHKALKRLRETIV"
BASE COUNT          868 a          895 c         1008 g          852 t
ORIGIN      
        1 tttggatgct ctctcgcttc ctgctcagcc cggagtcttt ggcatcagtc ttgccagatc
       61 tgactcgatg caaactgatc gggtcttgtt tgcttcgtcc taccgagacg gagacaagat
      121 gtctgcactt tcagggcacc tgaaacttat ccacaatatc aagagcggaa agcgagagta
      181 ctacgacctg caaaaggatc cgaaagaaac tcagccactc gacgagcctg atcgtcctga
      241 gttccagaac cttgatcacg ctttggaaaa gtggcaagaa atcaatgcgg cgcagcaacc
      301 gctcagcgca tccacagaac tgaccaatga gctatcagaa gagttgaaat cactcggata
      361 tcttcgctga gtgcaacaga ctggggcata aggaagtcgg gagtcactgg caactgcgat
      421 gaaactcaga gaacatcttc tttcggagca atggggctat tgggtggcta ccgtgatcgg
      481 agccgttgcg ctgttgatcg gcctgatcgt cagccgcacg gatgttggaa aagttcttga
      541 actgaggctt ctggatgcgc gtttcctgtg gagaggtcgt gctgaaccgg agagttccat
      601 cgtcgttctc ggaattgatg aggagagcat agacttcttt cgggacaagg ggaaaccctg
      661 gccttggcca aatcggattt atgccgaagc ggttcagtct ttgaccgaca tgggtgcatc
      721 ggtcattgtc tttgacaaga ttttcaagga tccgacttct ggttcgagcg atgaagatga
      781 gcaactgctc gcccagtctg ccaagaggca cggcaagacg atctatgcgg tggacatgga
      841 caaacgggaa gtgggtggaa tcatccgtga gtcagtcaac gagccacttg ctgtcatcgc
      901 tgagtcgggg attctgggat tcatcgatct acccatggac gaggatggag cagttcgtcg
      961 ggtgactttt ctggcctcgt gtcaggggaa gcaatatctc aaccttgacc tgcaagccta
     1021 cctgctcatc aaggggaaaa ctcaagacaa tgttcatctg actccgaaga agaatcttca
     1081 agttggaagt cttgcgatcc ctctggattt tcggaattca gcatatatca actatcctgg
     1141 caagagtgtc tttgaggagt acgctttctt tcaactgttc gatccggaac agaaggctct
     1201 tctgcaggag ttggagatct tccgcgggaa gactgttttc attggagtga ccagcgagtt
     1261 gtttcatgat gactacgtca ttccattcag ccgtgtctcg aaggcgcgct ccacctcgga
     1321 aactgaacgc gaatttgccg gaacgactcg ggagtatgga gtccatatcc acgcggcggt
     1381 tctgcacaca ttgctcaagg gacccagaat tcagccagtt cccctgaaaa tggtggggcc
     1441 gatctcaacc gctctgggac tgctggttgt gttactcttg agccgtgccg gattcaaact
     1501 gagtcttctt ctatcagtag gtctcgcgtt ttgctgtatg gtggctgggt tctggctttt
     1561 cctgcaccac gccgtgtata tgaaccttgt ctctcccctg tctgccatcg catttccttc
     1621 ggttgccatg ctctactaca agtttctcta cgaacaaagc cagaagagtc agattcgctc
     1681 ttactggtct cggtatgtct ccgatgacat tgtggatctg atggtcaaag gcaagatttc
     1741 tgtcgagtta ggtgggcaga gaaggcgggt cactgttctg atgtccgata tcagggactt
     1801 taccccgctg tgtgaacggc tctcacccag tgatctgatc gcgcagctga atgagtactt
     1861 ctccgagatg gcgcgggcga tctttgccca tggtgggaca attgataagt ttattggtga
     1921 tgccatcatg gcctattggg ggtttcccca tgaacaggat gatcatccac aacgggcttg
     1981 tctggctgcg gtggagatgc ttcgacatgc cgaacttctc tgcaaaaagt gggaagatga
     2041 ggggaaacca acctttcgga tgggggtcgg catcaacaca ggcgatgtgg tcattggcaa
     2101 tgtcggcgcc gaggttgaca atgagacgat ccagaacttc accctcctcg gtgatgcggt
     2161 gaacctggcc gcgcgacttg aatcggagaa caagaaccac gggacaacgc tcatcgtcag
     2221 tgagtacaca tgggaacagg tgagagatct caaaggcttt cgcgccagaa gccttgggga
     2281 actgaaagtc aagggcaaga gtcaggcact tcagtgttac agtattgaac tggacgaaac
     2341 agttcaaacg ggataaaatt ctctctcagt aagatagatt tgacggagga aagatatcat
     2401 gagaagcatc ctcattccca ctttgttagt cctgttgatc tgtgttgggg tctctgccca
     2461 tgctcaaggg caggctgtcg gcgacgcaaa gatcacgagt gtcagcggaa ccgtcatcgt
     2521 ccgatctgcg tcttcgtcga aactgactcc tgccaaacct gcgatggtcg tctctgtcgg
     2581 ggatacggtc atgacgcgaa aggcaagcga ggcgaccttg ctttttcaga agacaggggc
     2641 tttcaagaaa ctgggttcag atgagaacta cgttgtccag gtcgaacagc aggacagtgc
     2701 caccaagagt ggtctggggg atgtgctgtc gggtttgacg gagcttttca ccagccagag
     2761 tgacacgcgc gaggaactcc tggcgatctc tgcaacagtt cgcaaggcaa ccgacgagtc
     2821 gggtgttgtc aagaacaact acatctttcg aatgcctctg aataccgacc tgctggaaga
     2881 aactccgaca attcgctgga atcccattca ggatgcccgt gcttacagcg tctccatagc
     2941 ggatcccacg ggtgtctttc ttgagaaatc cactatggag actgccctgg atctggcagc
     3001 tttgaacaag ccccttgcaa gaaatgtcac ctatacggtc aaggtgcggg catcggttcc
     3061 ggacaaagga atcgttgagg attccggata cattcgagtc aagagcgtac aagaggtcag
     3121 ccagatcaaa caggccgagc agatgattca tgacgagacg ggcaagggtc tggacgagac
     3181 ttcgtcggct ctgattctgg gaagatatct cgaaagcagt cgcctctatc aaccggccta
     3241 tcagctctat gaacaaatgc tgaagaagga ctcttccgct gactttgcga aactccggat
     3301 ggagatcatc gaagtgacct gtcaggtcaa ggaaccgacc gtagagtcca actgactctc
     3361 gtgggtgtgc gttatgcagc agggctcact gtcgaacgcc gagattcggg aacgcctcgg
     3421 attctccgat gatcagctga tgcgtgagtg tgaactgcag ccctaccagg ccagcggtcc
     3481 cggaggacaa aagcgaaatc gaacgtactc cgcagttcgg ttgcagcatc atccaacagg
     3541 catcagcgtt actgctgccg aaagccgttc ccagttggac aaccgtcata aggcgctcaa
     3601 acgcctgcgt gagaccatcg tgg
//
LOCUS       DAOQUC010000230         3603 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_29376_length_3603_cov_3.336246, whole genome shotgun sequence.
ACCESSION   DAOQUC010000230 DAOQUC010000000
VERSION     DAOQUC010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3603
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29376_length_3603_cov_3.336246"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2493)
                     /locus_tag="PKH07_05200"
     CDS             complement(<1..2493)
                     /locus_tag="PKH07_05200"
                     /inference="COORDINATES: protein motif:HMM:NF016563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH116 family glycosyl hydrolase"
                     /protein_id="HNT34379.1"
                     /translation="MRWCFVVVFVITFSVLRVQSAVCQNQKTLSNGYYSVAVENGSIR
                     SLLCDPDGKGNFGVNILEKLAFDGIVASEETHCSPPAGDATPALKWTDMAAVQKGSIN
                     IKDCHIPVEVMPGSFLGQTFEVPGTFDSLAVKVPTWHSTTSSATIQLLKDGKVIAKER
                     FDPIQDNAYVRFRFEQQPAGQYEVRLLEPKGGRVGWWSADKDIYADGAGTSDGKADIS
                     CDRALEVPYSKAYSNKAEVSVILDNAKLSLAVAIPEPTEKIEIRHNAIFSVSWEWDGY
                     DTTPKGTPFKRVYSDQQRFMPIEQLKRNDHNPVLNCGQGFHFDGTGDYDLEMSCGRSS
                     LHWSYSKDHLGMTIRCPFTKDENGVTRATITLRVLPREDRFPDDWPAFETSDKDMDHD
                     LNLMLYERNFSYPSPCGPAPWIEWSAIARAWFAGAMRDGEKRHAADIVMDETGYVHTW
                     GGRPGWPFPDNNVYDTRHFDTNARLILSVWRYTLWTGDLNYLIEQLPRLRKAMQYQLT
                     VLDGTSGLIKTVSKDVNGRHRGVGNNYWDILPFGHLDAYANAAFYASLEAMAQLEEVF
                     EQRVAEEQRQSLNTETELHTPEYYRALRQKTLAEYTTKFWDEKEGRFIGCIDIDGNRH
                     DYGFTFLNLESMYYGLASPEQIKRIYNWMENGLSSTGKADIYSAWVFAPRATTIHNPM
                     WNNEHPDGLEENMTAKPWWHFGWTGTSFGDQCQDGGAIFYTSFFDLANRARFLGADDA
                     YRRWQEILVRYREPDRLCGGTPLFRGEVPQRVDPGAVGLDVPFPESGMVPCWVLYGMM
                     GIEPTPNGLRIKPNLPSQLEFCAVRNVSYRNLP"
     gene            complement(2535..3509)
                     /locus_tag="PKH07_05205"
     CDS             complement(2535..3509)
                     /locus_tag="PKH07_05205"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNT34380.1"
                     /translation="MPTNYSFGFSESLLSSVSGIPMDALHHDPDAMIRAAESVRPIAE
                     RLGVAPPRPHLAGFCYTPVAALGAKIVFPRGSEPKVYPLIQTPQQIDHLKEPEDYLAA
                     DLIQQRLAALSRLKQLCPTAGNHIGHSVEGPVTTAMLLMGQDFLLLLYDDPKRAHALL
                     DFSVRSALGYARAISMKLSGAIKSGVVGVPDDFSGMLPPELFPEFVVPYTNNLFEGLQ
                     ATERHYHSELLHNEHLPYLKDMKIDRFDPSTDQYLTPEQVAEDSPCRFMLRIHAWEVQ
                     DMSPNELVQYYKRLASLKPDLIGFSMWRVADEPKVRALLEVARELKGE"
BASE COUNT          828 a          949 c          969 g          857 t
ORIGIN      
        1 gggcagattc cgataggaca cattccgaac ggcgcaaaac tcgagttgag acggaagatt
       61 cggtttgatc ctcaggccgt ttggagttgg ctcgatgccc atcattccat agagaaccca
      121 gcaaggcacc atgccacttt ccggaaaagg aacatcgaga cccactgcgc ccgggtcaac
      181 tcgttgtgga acctctccgc ggaaaagagg tgtcccaccg cagagccgat ctggttcccg
      241 atagcggaca aggatttcct gccagcgtcg gtaggcgtca tccgctccca gaaatcgggc
      301 gcgatttgcc aaatcaaaga aggacgtgta gaagattgct ccaccatcct ggcactgatc
      361 gccaaaggaa gttcccgtcc atccgaaatg ccaccagggc ttggccgtca tgttttcttc
      421 gagtccatcg ggatgttcat tattccacat gggattgtgg atagtcgttg cgcgtggagc
      481 aaacacccaa gccgagtaga tgtccgcctt gcccgtggac gatagtccat tctccatcca
      541 gttgtagata cgcttgatct gctcaggaga ggcaagtccg tagtacatgg actccaggtt
      601 cagaaacgtg aatccgtagt catggcgatt gccatcaata tcaatgcatc cgatgaatcg
      661 gccttccttc tcatcccaga atttcgtggt gtattccgca agagtctttt gacggagcgc
      721 acggtagtat tccggagtgt gtagttcggt ttctgtattg agtgactgcc gttgttcctc
      781 ggcaactcgc tgctcaaaaa cctcttccaa ctgcgccatc gcttccaatg aagcatagaa
      841 agctgcattg gcgtatgcgt caagatgtcc aaacgggaga atgtcccagt agttgttgcc
      901 gacaccgcgg tgccgaccgt tgacatcctt gctgactgtt ttgatcagac cggaggtgcc
      961 gtccagtact gtcagttggt actgcatcgc tttccggagt cgaggcaact gttcaatgag
     1021 gtagttcaaa tcacctgtcc aaagcgtata ccgccagacc gacaggataa gtcgggcgtt
     1081 tgtgtcaaaa tgtcgtgtgt cgtagacgtt gttgtcgggg aaaggccagc ctggtctgcc
     1141 tccccacgtg tggacatagc ctgtttcatc catgacgata tctgcggcat gccttttctc
     1201 tccgtccctc atcgcgccgg caaaccaagc gcgtgcgatt gccgaccact cgatccacgg
     1261 tgcaggccca cacggtgaag gatagctgaa gtttcgttca tagagcatca gattcaagtc
     1321 gtgatccatg tccttgtcag atgtctcaaa cgcaggccag tcatcaggaa atcggtcctc
     1381 gcgtggcagg actctcaagg tgatcgtggc tctcgtgaca ccattctcat cctttgtgaa
     1441 aggacacctg atggtcattc ccaagtgatc tttggagtaa gaccaatgca gagaacttct
     1501 gccacaactc atttcaaggt catagtctcc cgttccatca aaatgaaagc cttgaccaca
     1561 attcaagacc ggattgtggt cgtttcgctt cagttgctca atgggcatga agcgttgctg
     1621 gtcgctgtaa acgcgtttga acggtgtgcc tttgggtgtg gtgtcatagc cgtcccattc
     1681 ccaggacacg gagaaaatgg cgttatgtct tatctcgatt ttctcagtcg gctctggaat
     1741 tgccacggca agcgagagtt ttgcattgtc cagtatgacg gagacttccg ctttgttgga
     1801 gtaggccttg gagtacggca cttcaagcgc ccgatcacag gagatgtcgg cttttccatc
     1861 agatgttccc gctccatccg catagatatc cttgtcggca gaccaccaac cgacgcgacc
     1921 ccctttgggc tccagaagtc ggacttcata ctggccggca ggttgttgct caaaacgaaa
     1981 tcggacgtag gcgttgtcct ggatcggatc gaagcgttct tttgcgatga ccttgccgtc
     2041 tttgagtaac tgaattgtcg cgctggaagt ggtgctgtgc catgtgggga ctttgactgc
     2101 caggctgtcg aatgtgccgg gaacttcgaa cgtctgtccg agaaatgacc caggcatgac
     2161 ctcgactggg atatgacagt ccttgatgtt gatggaaccc ttctgaactg ctgccatgtc
     2221 cgtccacttc agggctggtg ttgcatcacc ggccggtggt gaacagtggg tctcctcgga
     2281 tgcgacgatg ccatcgaacg cgagtttctc cagaatgttc accccaaagt ttcccttgcc
     2341 gtctggatcg cagagaagac ttcggatgga accgttctca acggcgacgc tgtaataacc
     2401 gttgctcaag gtcttctgat tctggcagac agcgctctga acacgcaaca cggaaaacgt
     2461 aatgacgaaa acaacaacaa aacaccaacg catgagaaaa ctccttgtct atttcacctg
     2521 cagaacggag cggtttactc gcccttcaac tctcttgcta cttcgagaag cgctctcacc
     2581 ttgggttcat cggcaactcg ccacatggag aatccaatca agtccggctt gagcgaagcc
     2641 agtcgcttgt agtactgcac caattcattg ggagacatgt cctgcacttc ccacgcatgg
     2701 attcgaagca tgaaccgaca aggggagtct tcggcaactt gttcgggagt caggtactga
     2761 tccgtgctgg gatcaaaccg gtctatcttc atatctttca ggtacggcag atgctcgttg
     2821 tgcagcagtt cgctgtggta atgacgttca gttgcctgga gcccctcgaa gaggttattg
     2881 gtatagggaa ccacgaattc cgggaacagt tcggggggca gcattccgga gaaatcgtcg
     2941 ggaactccaa caacaccgga cttgatggcg ccggagagct tcatagagat agcgcgcgcg
     3001 tatcccagag cgctccggac tgagaagtcc agaagcgcat gggcacgttt gggatcatca
     3061 taaagcaaca acaagaaatc ctgacccatc aagagcatgg cagtagtgac tggtccctcg
     3121 accgagtgac cgatgtggtt gccagcagtg ggacagagtt gcttcagccg cgacagtgct
     3181 gccagtcgct gttgaatcaa atccgcggca agatagtcct ccggttcctt gagatggtct
     3241 atttgctgcg gtgtttgaat caggggatac accttgggtt cagaacccct tgggaacacg
     3301 attttggcac ccagcgccgc gacgggtgta tagcagaatc ccgccagatg cggtcttggg
     3361 ggtgcgacgc ccaggcgttc ggcaatgggt ctgaccgatt ccgctgctcg aatcatcgcg
     3421 tcaggatcat gatgcagtgc atccatggga attcccgaaa cggaagacaa aagcgattcc
     3481 gagaagccga aggagtaatt ggtgggcatc ttgacaactc catcgaaaac ctgggcagac
     3541 aattccaggt ggattcattc aaaacgagac gcatcgtatc atgttccgtg caatcttggt
     3601 agc
//
LOCUS       DAOQUC010000231         3594 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_29471_length_3594_cov_3.287087, whole genome shotgun sequence.
ACCESSION   DAOQUC010000231 DAOQUC010000000
VERSION     DAOQUC010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3594
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29471_length_3594_cov_3.287087"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..494
                     /locus_tag="PKH07_05210"
     CDS             <1..494
                     /locus_tag="PKH07_05210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34381.1"
                     /translation="SLTGGNFFVRKEVLVQLNGFSPELGMCGDQIGLGDEDDVQFRMS
                     RDMPTEVRYYDPQLNILHWVRPDKMTLGFVLKGSFVSGQSAYRAHLGKRAERSWLVLL
                     CKQLTKALLGFVLFCVQRPLPSKHRRYQYFGNYVFENARSIVTTLGILSEKWGVGHKT
                     QRT"
     gene            534..1547
                     /locus_tag="PKH07_05215"
     CDS             534..1547
                     /locus_tag="PKH07_05215"
                     /inference="COORDINATES: protein motif:HMM:NF024914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNT34382.1"
                     /translation="MKVLHVGVFRDHALGLDHVCRSGFLKNGIDPICFDFRSSAEEMG
                     LERMNQSLLEQGADSDLIFITKGELIQPLTLKRLREKGAQVSIFYGDIRQEPENWLVD
                     NCPHCDVFFMTSAGDVLKHYYEVCKPSRAAFVWNPSNPELPDEFRDVPRSVDPPLFTA
                     TVHPHMGSERRAVYEYLCRRRDVSMVGCSDRVFRWDILNRLYYKIHPPHYLRGRDYIE
                     RIIRSRFGIGVSAMQNVKYYTSDRLVHYLTFGKCFLAYRFPGCEDVLQNGVHVVYYDG
                     IDDLARKIDYYLEHPDQAEKIGVSGQRKVLEEYNAERIVHLMLDILRSGGSNLFPWVE
                     VYG"
     gene            1558..2283
                     /locus_tag="PKH07_05220"
     CDS             1558..2283
                     /locus_tag="PKH07_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005814360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNT34383.1"
                     /translation="MNDDPEVSRFFRRTDPHEDRFVFDLPKSWWSRPYEYAWAASFAQ
                     ESDTALDAACGICHPLKFHLSDTCRVVHACDLDERILSPEAIKKDIISDFGDSALDTL
                     PTRYFDKISYAQASILSLPYADESFDKIYCISVLEHLRDFGNRYPLADLFLSRLSSFL
                     PYQIGGALKEFRRVLRDQGRVILTFDYPKINLGYLNRAVIRAGLDFVRRPDFTLPDDA
                     LQDPKGQLYCFRTVLRKAHEGKP"
     gene            2280..3497
                     /locus_tag="PKH07_05225"
     CDS             2280..3497
                     /locus_tag="PKH07_05225"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNT34384.1"
                     /translation="MNIGIVTAWCDVGAGMVSRAYRDVLQTKHNVFIYVRGGLQPRDD
                     PNWQAPNVTWGRQFRRGITRVALKEFHRWAVQHKLDVILFNEQWNFIPVVYARRHLDV
                     ALGTYVDYYKANTVAFFDLYDFLLCNTRRHHSVFQHHPHAVYIPWGTDCELFNGSCEC
                     VQQGSVVFFLSAGGNPVRKGAETAILGFKLLRGNCRFVLHLQRDLSSFPTLELLCRDD
                     PRIQVVVRNVPPPGLYHHGDVYVYPSILDGIGLTIPEAMACGLPVITTDCAPMNEFVQ
                     NGVNGRLVQPYEYRTRPDGYYWPESYCRPEDVAEAMQHYVDHLDHLGEFKKQSREYAR
                     THHDWFKNALALPEMMNGFSPSQIERQRQTELEWQALDDSYLPPMRRNFRDVLRLMNA
                     HHGHLADWLLYSR"
BASE COUNT          827 a          895 c          957 g          915 t
ORIGIN      
        1 agtcactcac cggcgggaat ttctttgtgc gaaaagaggt ccttgttcag ttgaatggat
       61 tcagtccgga gctggggatg tgtggcgatc agattggctt gggtgatgaa gatgatgtgc
      121 agtttcgaat gagtcgcgat atgccgacgg aggtgcgcta ctacgatccg caattgaaca
      181 ttctgcattg ggtcagaccg gacaagatga cgcttggttt tgtcctgaag ggctcctttg
      241 tctccggaca gtccgcctac agagcgcacc tgggcaagcg ggcggagcgt tcctggctcg
      301 tgctcctgtg caagcaactg acaaaggctc tcttgggttt tgtgctgttc tgcgtgcagc
      361 gtcctctgcc gtcaaagcac cgccggtatc agtattttgg aaactatgtt tttgaaaatg
      421 ctcgaagtat cgtcacaact ttgggcattc tttcagagaa gtggggcgtt ggacacaaga
      481 cgcaacgcac atagactccc ccacgggagc acgggaagag gaaaccacca acgatgaaag
      541 tcctgcatgt cggcgtgttc agagaccacg cacttggtct cgatcatgtc tgtcgaagtg
      601 gttttctgaa gaatgggatt gatccgatat gctttgactt tcgttcatcc gctgaggaga
      661 tgggtctgga gcgtatgaat cagagtctgc ttgagcaggg cgctgattct gatctcatct
      721 tcatcaccaa gggcgaactc atccagccat tgacgctgaa gcgtctgcgg gaaaagggag
      781 cgcaggtttc catcttctat ggagatattc gccaagagcc tgaaaactgg cttgtagaca
      841 actgcccaca ctgtgatgtg ttctttatga cctcggcagg tgacgttctg aagcactact
      901 acgaagtgtg caagccatca agagcggctt ttgtctggaa cccaagcaac ccggagctcc
      961 ccgacgaatt cagagatgtt ccccgttccg tggatccgcc acttttcacg gcaaccgtac
     1021 acccgcacat gggatcagaa agaagggcag tctacgaata cctttgtcgc cgaagagatg
     1081 tcagcatggt cgggtgttcc gacagagtct tccggtggga cattctcaat aggctctact
     1141 acaaaattca tccgccccat taccttcgcg gtagagatta catagagcgt atcattcgct
     1201 cgcgttttgg cattggcgtg agtgccatgc agaacgtgaa gtattacacg tccgatcgcc
     1261 tggttcacta tttgacattt ggaaagtgct tcttggcata tcgttttccc ggctgcgagg
     1321 acgtacttca gaatggagtt catgtggtct attatgatgg catagatgat cttgctcgca
     1381 agattgacta ctatctggag catcccgatc aagcagagaa gattggggtc tctggtcaga
     1441 ggaaagtgct agaggagtac aatgccgaac ggatcgtgca cttgatgctg gacattctcc
     1501 ggagcggggg atcgaatttg tttccgtggg tcgaggtgta tggttgagaa cgtgttgatg
     1561 aatgatgatc cggaagtctc acggtttttc cgaagaaccg acccgcatga agatcgtttt
     1621 gtgttcgatc tccccaaatc ctggtggagt cgtccttacg agtatgcttg ggctgcatcc
     1681 ttcgctcagg aatcggatac agccttggat gctgcgtgcg gcatctgtca tccactcaag
     1741 ttccatctgt cggacacctg tcgagtagtt catgcctgtg atctcgatga gagaatcctt
     1801 tcaccggaag cgatcaagaa agacatcatt tcggattttg gcgattccgc gcttgacact
     1861 cttcccacaa gatactttga caagatcagt tacgcccagg cgagcattct ctcgctgccc
     1921 tatgctgatg aatcttttga caagatctac tgcatttccg tgttggaaca tctgcgtgac
     1981 tttggaaatc gctatccact cgctgacttg ttcctgtctc ggttatcttc gtttcttccc
     2041 tatcagatcg gtggtgcatt gaaggagttt cgcagagtgt tgcgggatca aggacgcgtg
     2101 atcttgactt ttgactatcc gaaaatcaac ctcggatacc tgaaccgcgc tgtcataagg
     2161 gcaggccttg atttcgttcg acggcctgat ttcacattgc ccgacgatgc acttcaggat
     2221 cccaaaggac agctgtactg ctttcggact gtgctcagaa aagcgcatga gggcaaacca
     2281 tgaacatcgg tattgtaaca gcgtggtgcg atgtgggtgc cggcatggtc tcaagggcat
     2341 atcgggatgt actccagacg aagcacaacg tattcattta tgtacgcggc gggcttcagc
     2401 ctagagatga tcccaactgg caagccccta atgtgacatg gggaagacag tttcgacgtg
     2461 ggataacccg agttgctctg aaagaattcc accgatgggc tgttcaacac aaactggatg
     2521 tcatcctctt caacgaacag tggaatttca tcccggtggt ttatgcgcgt cgtcatctgg
     2581 atgttgcgtt gggaacttat gtggactact acaaagccaa taccgttgca ttcttcgatc
     2641 tgtacgattt ccttctatgt aacacgcgca ggcatcattc ggtctttcag catcatcctc
     2701 acgcggtgta cattccttgg ggcacagatt gtgaattgtt caacggatcc tgtgaatgtg
     2761 tgcagcaggg cagtgtcgtg ttcttccttt ctgcgggcgg aaatcccgtg cggaagggcg
     2821 cagagacagc catcctggga ttcaaacttt tgcgcggtaa ctgccgcttc gtcctgcatc
     2881 tgcaaagaga cttgagttcc tttccgacgc ttgagctcct gtgtcgcgat gatccccgca
     2941 ttcaggttgt tgttcgcaat gttcccccac ctgggctgta tcatcacggt gatgtctacg
     3001 tctacccgtc aattctggac ggtattggct tgacgatccc cgaagcgatg gcttgcggac
     3061 taccggtgat tacaaccgac tgtgccccaa tgaacgagtt cgtgcagaac ggagtcaatg
     3121 gacgcctcgt gcagccctat gaataccgaa cccgtccgga tggatattac tggccggaga
     3181 gctactgcag acccgaagac gtagctgaag ccatgcagca ttacgtggat catctggacc
     3241 accttgggga gttcaagaaa cagtctcgtg aatacgctcg aacacatcac gactggttca
     3301 agaacgccct cgcgctacct gaaatgatga acggattctc gccatcgcaa atcgaaagac
     3361 aacgacaaac tgaactggag tggcaagcac ttgatgattc ctatctgcca ccaatgagac
     3421 ggaactttcg agatgttctg cgcctgatga atgcacacca tggacacctt gccgactggc
     3481 ttctgtacag tcgctgacac agaaacacgc ccgagtgact gtagacccta tatctggcaa
     3541 agagtatttc aaaccgaccg acccgaacgg ttaggaacgg ctccgctatc gcgg
//
LOCUS       DAOQUC010000232         3591 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_29503_length_3591_cov_3.527715, whole genome shotgun sequence.
ACCESSION   DAOQUC010000232 DAOQUC010000000
VERSION     DAOQUC010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3591
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29503_length_3591_cov_3.527715"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1863)
                     /locus_tag="PKH07_05230"
     CDS             complement(<1..1863)
                     /locus_tag="PKH07_05230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34385.1"
                     /translation="MNMIRLECQRKRCCAVLIVLALIVVFHLSTPVAQGQVPSVWQSS
                     LLPGNQTLEKSVSSNEQAFVRVGTVHAPAGAGLFGSPSLMSALLFDEDWESYSSTSQV
                     FGAGGWVHSTYGNATQTLGVHSGTGHAGGISPTNVLYCNDGDGNAVHLFPATYPSDSE
                     PLVVDVWMYDEGGTNNFKGVSLIRTNDGGLTQSGRVCIHRFTDKGMYWRLRGDGLTLE
                     QFAGPGIGPGWRRIVITVGSNSCVVNVNGSTAGATSGYSFVPTGGWNAVALGYENNSA
                     YTGGEAFFDEIMVYTGQAPTMTPTPVATDTPAPADLIVDNLDAGYSDHQGDTPWITGS
                     SGSSFYGTDYRYNLTGTGLDTATWTTNIPTAGMYEVFVWYSAAINRANNAPFTVHHVG
                     GTQTVLVNQKTGGGEWYSLGVFAFSAGSADVVLSDQAEPGMVVIADAVRWLSQGITPT
                     PTSTPTPTYPPQDGAYPNLGSWRELFVDDALIEQASGLNLEMHSPEPREVVITFDAPW
                     EGSNSAYVTVFKDENLYRMYYRGNKPGNPQVTCYAESTDGINFTKPVVGIFNFGGNTN
                     NNIILVDEGTHCFAPFKDTNPAAPASERYKAVSRGDGGLIAFVSADGIHWSKLQE"
     gene            2162..>2870
                     /locus_tag="PKH07_05235"
     CDS             2162..>2870
                     /locus_tag="PKH07_05235"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HNT34386.1"
                     /translation="MRPNSCWTSLTALMALALLFALAPSVSSSQEPVVSDDYGSTLVV
                     KKPFQRVISLTPSVTEDIYSLGAQDQLVGVTTYCDYPPQARYKPQVGDIVRPNVERIL
                     NLNPDLILCAGNALQVTKKLADMGVPCLVFRSESLADIRHDLQRLGTLLGREEQARTL
                     CLDMDEQIKTVRTNAALRQTPKAVLAFDLKPLIVAGEKTLGDELLNLIGCQNVGRAAQ
                     GRYPNLSVEFLIQERPEF"
     assembly_gap    2871..2880
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2881..3103
                     /locus_tag="PKH07_05240"
     CDS             <2881..3103
                     /locus_tag="PKH07_05240"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34387.1"
                     /translation="FFFYAGKGTEEETLALRSEWVEWTSIPAVSKGHIYSVSADLVSR
                     PSLRLLEGLRGLEKILGEETQPVALGEED"
     gene            3106..>3591
                     /locus_tag="PKH07_05245"
     CDS             3106..>3591
                     /locus_tag="PKH07_05245"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HNT34388.1"
                     /translation="METQQTTSSHNLKKRFRSHVLLPLSVLLVFLLCACVLNVCIGPA
                     HISFSEVSDGLFQPRGEMTPGAQIFYHLRLPHLLLACLIGAVLALVGAALQALFRNPL
                     AEPFMIGISSGSSLAVVVATSLGIHALSFGKFGHYLVPLFAFAGGLGSLALVYWLATR
                     RG"
BASE COUNT          781 a          978 c          961 g          861 t
ORIGIN      
        1 ctcctgcagt ttcgaccagt ggatgccgtc tgcggacaca aaggcaatca agccgccatc
       61 accacgactg accgccttgt atcgttccga ggcgggagca gcgggattgg tgtctttgaa
      121 gggcgcgaag cagtgtgtgc cttcgtccac gaggataatg ttgttgttcg tgttgcctcc
      181 gaagttaaat atgccgacaa cgggtttggt gaagttgatg ccgtcggtgc tctctgcgta
      241 gcaggtcact tgaggattac ctggcttgtt cccacggtag tacatgcgat agagattctc
      301 atctttgaaa actgtgacgt aggcgctgtt cgaaccttcc cagggagcgt caaaggtaat
      361 cacgacttct ctcggctccg gactgtgcat ctcgagattc aggccggaag cctgttcaat
      421 gagagcatca tctacgaaca actcccgcca actgcccagg tttggatacg caccatcctg
      481 tgggggatag gtcggtgtcg gtgtggatgt aggcgttgga gtgatgccct gcgacagcca
      541 acgcacggca tcggcaatga ccaccatgcc aggctccgcc tgatcactca gaaccacgtc
      601 tgccgatcct gcgctgaagg caaaaactcc caatgagtac cactcaccgc cgccggtctt
      661 ctgattcacc aatacggtct gggttccccc tacatggtga acagtaaagg gtgcgttgtt
      721 ggctcggttg atcgcggcgg agtaccagac aaagacttca tacattcccg cggtaggaat
      781 gttggtcgtc caagtggccg tatccagacc ggttccggtg aggttgtagc gatagtccgt
      841 tccatagaaa ctcgatcccg acgatcccgt tatccaggga gtgtctccct gatgatcact
      901 gtaacctgcg tcgagattgt ccacaatgag atccgccggt gccggtgtgt cagtggcaac
      961 gggtgttggt gtcattgtcg gagcctgacc agtgtagacc atgatttcat cgaagaaagc
     1021 ttccccgcct gtgtaggcgc tgttgttctc gtatccgagc gccacagcat tccatccacc
     1081 cgtcggaaca aaggagtaac cggaagtagc gcctgcagta gagccattga cgttcacgac
     1141 gcaggagttg ctgccaaccg tgatcacaat gcgtcgccac ccaggaccga ttccaggtcc
     1201 agcaaactgt tccaaggtca gaccgtctcc acgaagacgc caatacatgc ccttgtcagt
     1261 gaagcgatga atacacaccc gacctgactg agtcagtccg ccatcgttgg ttcgaatgag
     1321 gctgactcct ttgaagttgt tggtgcctcc ctcatcgtac atccagacgt ccactaccag
     1381 tggctcactg tctgagggat atgtggcagg aaacagatga acggcattcc catcgccatc
     1441 attgcagtag aggacgttgg tcggagagat tcctccggca tgacctgttc cactgtgaac
     1501 acccagagtc tgagtggcgt tgccataggt ggaatgaacc cagccccctg ctccaaagac
     1561 ttgcgaggtg cttgaataac tctcccagtc ttcatcgaac aaaagagcac tcatcaatga
     1621 gggactgccg aacaacccag cgcctgcagg cgcgtgaact gtgcccactc ttacaaacgc
     1681 ttgctcattg gaagaaacag acttctccaa agtctggttt cctgggagca aagacgactg
     1741 ccagacgctc ggaacttgac cctgcgcaac gggtgttgaa aggtgaaaaa caacgatcaa
     1801 cgccagaacg atcaggacag cgcaacaacg ctttctttga cactccaaac gaatcatgtt
     1861 cattcggcct tccctgaaag atgtcttcgc tgcagcgctt tcccctgtcc agaggagaac
     1921 tctgaaaaca cccctttttg ctgttgcgaa gagaacaggc aggtcacgac acatcctgcc
     1981 acagttcatc ggacagccga ttctgcacac cggttgtcca aatgatggta ccttaagaca
     2041 agaatagagt ctctataccc cttttgtcga caatttcttt tctcacgacg actggctctt
     2101 gaaataatcc ccttggaacg ccatacttcc gttggttttt gaaaactggt gaggatgttg
     2161 catgagaccg aattcctgct ggacttcttt gactgccttg atggcgcttg cgctgctgtt
     2221 cgcactcgcg ccttctgtgt ccagttcaca ggagccggtg gtcagcgatg actatggcag
     2281 cacccttgtc gtcaaaaagc cgtttcagcg cgtgatctct ctgactccca gtgtcaccga
     2341 agacatctac tcactgggtg ctcaagacca actggtgggc gtcacaacct attgcgacta
     2401 tcccccacag gcaaggtaca agccccaggt cggtgacatc gtccgtccaa atgtggagcg
     2461 gatattgaac ctgaatcctg atctgattct gtgtgctgga aacgctctgc aggtgacgaa
     2521 aaagcttgcc gatatgggcg ttccctgtct ggtgttccgt tctgaatcgc ttgccgatat
     2581 tcgacacgat cttcagcgac tgggcacact cctgggcaga gaagaacagg cacggactct
     2641 gtgtcttgat atggatgagc agatcaaaac cgtgcgtacg aatgctgcgc tcaggcagac
     2701 gccaaaggca gtccttgcct tcgatctcaa gccgctcatc gttgccggtg aaaaaaccct
     2761 cggcgacgaa ctgctgaact tgatcggttg ccaaaacgta ggtcgagccg cccagggtcg
     2821 gtatccaaac ctgagtgtgg agtttctcat tcaggagcgc ccggagttct nnnnnnnnnn
     2881 gttctttttc tatgccggta agggaaccga agaagagacg ctcgctcttc gatctgaatg
     2941 ggttgagtgg acttcaattc ccgcagtgtc caagggccac atctactcgg tttccgcaga
     3001 tttggtctcg cgtccaagtc tgagactcct tgagggtttg aggggtcttg agaagatact
     3061 gggagaggaa actcaaccgg tagcgttggg tgaagaggac tgaacttgga gacgcaacag
     3121 accaccagtt cacacaatct caagaagcga tttcgttctc atgtgcttct gcccctgtcc
     3181 gtcttgctgg tctttcttct gtgcgcctgc gttctcaatg tgtgcattgg ccctgcgcac
     3241 atttcgtttt cggaagtgtc cgatggtctg ttccagccgc ggggcgagat gacaccggga
     3301 gcacaaattt tctaccatct tcgactccct catttgctcc tcgcgtgtct gattggagcg
     3361 gtgctggcat tggtcggagc ggcccttcag gcgcttttcc gcaaccctct ggcagaacct
     3421 ttcatgatag gcatctcgag cggatccagt ctcgcggtgg tcgtggcgac ttctctggga
     3481 atccatgctt tgtcgttcgg aaaattcggt cactatctgg tgcctctgtt tgcctttgcg
     3541 ggcgggttgg gaagtctggc gttggtgtat tggttggcga cacgccgtgg a
//
LOCUS       DAOQUC010000233         3591 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_29512_length_3591_cov_2.783937, whole genome shotgun sequence.
ACCESSION   DAOQUC010000233 DAOQUC010000000
VERSION     DAOQUC010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3591
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29512_length_3591_cov_2.783937"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..617
                     /locus_tag="PKH07_05250"
     CDS             <1..617
                     /locus_tag="PKH07_05250"
                     /inference="COORDINATES: protein motif:HMM:NF023255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3347 domain-containing protein"
                     /protein_id="HNT34389.1"
                     /translation="GAFKIDSDLQIRAGRSMMNPEPSTAHRTHDGQQTPQEPSSDVTS
                     LKEPAPLGFRRQLDMVLKSYYAVQASLAADNLEQAREAVPMLTEALDKVSLDLLQEKH
                     RALWTREKKTLSQAAAGVRDSKEIAKARESFLVLSDSLTETIRQMGVSGEVPILQFHC
                     PMAFNNRGANWLQESSELRNPYFGSSMLRCGTRTEILSEGKATQ"
     gene            670..>3591
                     /locus_tag="PKH07_05255"
     CDS             670..>3591
                     /locus_tag="PKH07_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018125811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNT34390.1"
                     /translation="MSEPPIGQQELTAHSWVDRLILFCLQKKIVVFVILLLIVWWGLM
                     VAPFDWHLGGLPRDPVAVDAIPDIGENQQIVFTEWMGRSPRDVEDQITYPLTSALLGV
                     PGVKTIRSYSMFEFSTIYVIFHEKVDFYWSRSRILEKLNSLPSGLLPEGATPILGPDA
                     TALGQVFWYTLEGRDKEDKPTGGWDLHELRSVQDWHVRFALLGVEGVSEVASVGGYVQ
                     QYQVDVDPDAMRAAGVSLEEIIDAVRLSNTDVGAQTIEINKVEYIIRGLGFVKTLEDI
                     ENAVVKVNGHVPICVKHVARVTLGPGVRRGVLDKEGTEVVGGVVVARYGENPLATIKN
                     VKRKIEEINPGLPRKTLADGTMSQVRLVPFYDRSVLIQETLGTLSRALLEQILITMII
                     VLAMRTHLRTSLLISTQLPLAVLLCFIAMKTFGVDANIVALSGIAIAIGTVVDMGIVL
                     CDNILTHLQEAAPGEDRLAVVYRASSEVGGAVLTAVATTIVGFLPVFAMVGPEGKLFR
                     PLAFTKTFALLASIAIALTVIPSLAHLFLGEQRRRRTLTERTLLYAPLLLLGAVLALK
                     AGFYWAGGLLILWAIYGFSGSRLPAAIQEIARRGATWAVIGVVVIVLASRWLPLGPDR
                     GLGRNVLFVALLILGLLLPFYLIQRFYSAILGWCLRHKLLFLSLPALLLLLGGVIWLG
                     FDAVFGWLPSEVSSRPAIVALRHEFPGLGREFMPPLDEGSYLYMPTTMPHASIGEAID
                     VLRKQDIALRGIPEVESVVGKIGRAESPLDPAPISMTETVITYRTEFLSDASGKRLSY
                     KFVHDETDFFRDVDGQTVAAPDGIPYVVQGRFVRDEMGQLIPDKNGKPFRLWRPALET
                     SLNPGRKTWEGIKKPDHIWDAIVKAAQIPGSTSAPRLQPIAARIVMLQSGMRAPMGVK
                     IKGPTLESIERTAGDVANLLKQVYSIEPSAVIEDRIVGKPYLEIAIDRHAIARYGLRI
                     ND"
BASE COUNT          755 a          918 c         1023 g          895 t
ORIGIN      
        1 atggcgcttt caagatagac agcgatctcc agattcgtgc cggaaggagt atgatgaatc
       61 ctgaaccctc gacggcacac agaactcatg acgggcagca aacaccccaa gagccttcat
      121 ccgatgtgac ctcactgaag gagcctgcgc ccttgggatt tcgccgacaa ctggatatgg
      181 ttctgaagag ttactatgcc gttcaggcat ctcttgcagc ggacaatctg gagcaggcac
      241 gagaggcagt tcccatgctt acagaggcgc ttgacaaggt cagcctcgat cttcttcagg
      301 aaaagcaccg cgcgctgtgg actcgggaaa agaagactct gagtcaggcc gcggcaggtg
      361 ttcgagattc gaaggaaatc gccaaggcgc gcgagtcttt tcttgttctt tcggattccc
      421 tcacggagac gattcgacag atgggtgtga gcggtgaagt tccgattctg cagtttcact
      481 gtccgatggc gttcaacaat cggggagcca actggctcca ggaatcatcc gagctgagga
      541 acccgtactt tggcagttcg atgcttcggt gtggaactcg gacagaaata ctctccgaag
      601 gcaaggcaac ccaatgagag gggcatctct tcctcttgtc atcgtgcgtt gcaagactgg
      661 agaagcaaat tgagcgagcc accgattgga caacaagaac tgaccgcaca ttcatgggtt
      721 gacagactca tcctcttttg tcttcaaaag aagatcgttg tcttcgtcat actcctgctg
      781 atcgtctggt ggggtctgat ggttgctccc tttgactggc atctgggtgg attgcctcgt
      841 gatccggttg ctgttgatgc catccccgat attggggaaa atcagcaaat cgtctttact
      901 gaatggatgg gtcgttctcc tcgggacgtt gaggatcaga tcacctaccc tctgacttca
      961 gcgcttctgg gagttccggg agttaagacg atccgcagct actccatgtt tgagttttcc
     1021 acgatctatg tgattttcca tgagaaggtg gatttctact ggtcacgctc tcgcattctg
     1081 gagaagctga acagtcttcc ctccggtttg cttcccgaag gtgctacgcc catcctcggt
     1141 cccgatgcca cagctcttgg gcaagtgttc tggtacacgc tggaaggcag ggacaaagag
     1201 gataagccca cgggtggctg ggatctccat gaactcagaa gcgtccagga ctggcacgtg
     1261 cggtttgcgc tccttggagt tgaaggtgtc tcagaagtgg cttctgtggg cggatatgtg
     1321 cagcaatacc aagtggacgt agatccggac gccatgcgtg cggccggcgt ttccttggaa
     1381 gagattattg atgctgtcag actatcgaat acggacgtcg gtgcacagac gatcgagata
     1441 aacaaggtgg agtacatcat ccgtggtctc ggttttgtca agacgcttga ggacatcgag
     1501 aacgctgttg tcaaggtcaa cggccacgtt cccatctgcg tgaaacatgt ggcaagggtc
     1561 acccttggcc ccggggtacg acggggtgtc ttggacaagg aaggaacgga agttgtcgga
     1621 ggcgtggttg ttgcccgata cggtgaaaac cctcttgcca cgatcaagaa tgtcaaacgc
     1681 aagatagaag agataaatcc aggactgccc cggaagaccc ttgccgatgg cacgatgagc
     1741 caggttcgcc tcgttccatt ctatgatcgc agtgttctga ttcaggagac attgggaacg
     1801 ctcagccgag ctcttctcga acagattttg atcaccatga tcatcgtgct ggcgatgcga
     1861 actcatctgc ggacttcgtt gctgatttcc acgcaactgc ctttggccgt gctcttgtgc
     1921 tttatcgcga tgaagacgtt cggtgtggac gccaatatcg ttgcgctgtc gggaattgcc
     1981 atcgcgatcg gtacagttgt ggacatgggt atcgttctct gtgacaacat tctcacgcac
     2041 cttcaggaag ccgctccggg tgaagatcgc cttgcagttg tttacagggc ttccagcgag
     2101 gtgggcggag cagtcttgac tgcggttgca accacgattg ttggttttct tcccgttttc
     2161 gcgatggtgg gacctgaagg caaactcttc cgtccactgg cgtttaccaa gacctttgct
     2221 ttgctggcat ccattgccat tgcgttgact gtcatcccat ctcttgctca tctctttctt
     2281 ggggagcagc ggcgtagaag gactctcact gagcggactc ttctctatgc gccacttctt
     2341 ctgctgggcg ctgttctggc tttgaaagct ggcttctact gggcgggagg ccttcttatc
     2401 ctctgggcta tctatgggtt ttccggaagt cggttgcccg cagctatcca ggaaattgcg
     2461 agacgtggag caacgtgggc tgtcatcgga gttgttgtca tcgttctggc gagccgttgg
     2521 cttcctcttg gccccgacag aggtctcggt cgaaatgttc tgtttgtcgc cttgctgata
     2581 ctgggtcttc tgcttccgtt ctatctgatc cagcgattct attcagcgat tctcggttgg
     2641 tgtctcagac acaaattgct ctttctgtcg cttccggctc ttctcctgct cttgggcgga
     2701 gttatctggc tcggattcga cgcagtgttc ggctggctgc cttccgaagt gagcagccgg
     2761 cctgccatcg tcgcgcttcg acatgagttt ccgggactgg gcagagagtt catgccccca
     2821 ttggatgaag gttcttacct ctacatgcca acgactatgc cacatgcttc cattggcgag
     2881 gcgattgatg ttttgagaaa acaggacatt gcgctccggg gtattccaga agtggagtcg
     2941 gttgtgggga agattggccg tgcagagagt ccacttgacc cagcgccgat ttctatgacc
     3001 gaaaccgtga tcacctatcg aacggagttt ctgagcgatg ccagtggcaa gcggctcagc
     3061 tacaagttcg tccatgatga gaccgacttc ttccgagacg ttgacggaca gacggtcgca
     3121 gcgcctgacg ggattccgta tgtggttcag gggcgatttg tccgtgatga gatgggacaa
     3181 cttattcccg acaaaaacgg caaacctttc agattgtggc ggcctgcact ggagacctct
     3241 ctgaacccag gacgaaagac ttgggaaggc attaagaagc cagaccacat ctgggatgca
     3301 atcgtcaaag cggcacagat tccgggttcc acttccgctc caaggctgca acccattgct
     3361 gctcgcattg tcatgctgca gagcggaatg cgcgctccga tgggtgtcaa gataaagggg
     3421 ccgacacttg aaagcatcga acgaacggcg ggcgatgtgg caaacctgct gaagcaggtc
     3481 tattcgattg agcccagtgc ggttatcgag gacaggattg tcggcaaacc ctatctggaa
     3541 attgccattg accggcatgc gatcgcccga tacgggcttc gtatcaatga c
//
LOCUS       DAOQUC010000234         3589 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_29538_length_3589_cov_3.147708, whole genome shotgun sequence.
ACCESSION   DAOQUC010000234 DAOQUC010000000
VERSION     DAOQUC010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3589
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29538_length_3589_cov_3.147708"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="PKH07_05260"
     CDS             <1..363
                     /locus_tag="PKH07_05260"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNT34391.1"
                     /translation="RLTSRYGWRTRPMGPTERKHFHTGVDIAAPHGRTISAAESGQVL
                     SACYAGALGNSVLISHRSGYSTLYGHAAKMLVRPGQYVKKGETIALVGSTGRSTGPHV
                     HFEVLKNGKHIDPMQVLH"
     gene            complement(709..>1098)
                     /locus_tag="PKH07_05265"
     CDS             complement(709..>1098)
                     /locus_tag="PKH07_05265"
                     /inference="COORDINATES: protein motif:HMM:NF012622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I domain-containing protein"
                     /protein_id="HNT34392.1"
                     /translation="FSPTNTPTVTPTRTATPTNSPTGTPTNSPSRTPTHTPSRTPSTT
                     PTWSPTVTPTTSGVTVPSNTPTATPTPQTRSADFDSDGDIDGRDLMVLLKMLNTTGDA
                     QTDLNGDGVTDMLDLMLFASQWNPNGS"
     assembly_gap    1099..1198
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1199..3527)
                     /locus_tag="PKH07_05270"
     CDS             complement(<1199..3527)
                     /locus_tag="PKH07_05270"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNT34393.1"
                     /translation="MMELIYDMAPGVTTLGFKTAYYSQADFANNITHLANSGMHIIND
                     DVGYGGEPWFQDGVIAVAINNFVDGGGVYFGSAGNNSQDFTYQRVFRDTNGNDWHEFE
                     GTDEVLSLVVANSKNAYCDLQWTQPWGNATTNMQIGFFRNGTLIAESTENNIGGNPND
                     TLSWTNNTGSDAQVDVIIRRAAGASAAGLTLKIIFGGKSMEYQGLYPGGSIVAHVGGS
                     KGFAVGEIPYTDPDNAGKATTYGSCPGPHTHFFNPSGTPYPTPIVYQKPDFMGIDCVN
                     TSFFGGDIDGDGLPNFCGTSAAGPNVAAVAALLQDLKGTGGSLSATQIHDLLRRSAVD
                     LKASGHDRWFGWGRVHAVGAAMLAKGPEPYEFNLYPNHMGDVVYEADFYSTADIDIVR
                     AAFRSGTSVNVTVQSLDGKSDPMIGIFKEASNDFVALDYDSGTGNAASLSFFSAAQIP
                     HRYQLLTETALTGTNAQYRLTINGGNQYTADVELDALGYANISSALGSNFHINYYRVN
                     TAHFGNYNGRLSLSLSPVGFQGMLIVFNDTTGAMLAQQYGSGVGTNVSYTLENTNAAT
                     YVVAVSSSEYSGKGNYQLSTMLTSYTPTPTRTATRTRTPTNTRTLTPTATPTFTYTNT
                     PEDTPTATPTQTPTRIPRFTDTPTGITKPTKTDTPTSTPTSFIRPSRTPTPTDEAVPT
                     GTDTPTATPTDEGQPTQTNTPSATPTRTSTNSPTVTPSSTPTYTHTETPTSTGTPSAT
                     PTPTVSSTPTNSPTDNPAFSPTNTPTVTPTRTATPT"
BASE COUNT          709 a          866 c         1010 g          904 t
ORIGIN      
        1 cgtttgactt ctcggtacgg ttggcgaacg aggccaatgg gacctacgga aaggaagcat
       61 ttccacacgg gagttgacat tgccgctccg cacggacgga cgatttctgc ggcggagtct
      121 ggtcaggtgt tgagtgcctg ctatgccggg gcgcttggca acagtgtgtt gatctcacac
      181 cgttctgggt acagtacgct ctatggtcat gcagccaaga tgctcgtacg acctggccag
      241 tatgttaaga agggtgagac cattgccttg gtcggttcaa caggccgttc aactgggcct
      301 catgtgcatt ttgaggtcct caagaatggc aaacacatag atcccatgca ggtgcttcac
      361 taggcgccgg tttccgtcat acctacgctt cccaattcaa aactagtctt gatgttgctg
      421 gtttccaagt atcatgaggt tgctcgatca ggaatcatac cgctgaagtt tacacggttg
      481 gtcaagttcg cccttgcggt cggtttcaaa ccattccagt cagtatccga cggtgacagc
      541 cctcttccct gtgcctgtct catagtgcat ctgcaaacga gattcctgtg caaggcaccc
      601 atccggctga acaagagaca tctcagttgc tgaaatgggt tagcctgaga ctcttgtgtt
      661 cccagttctt gccggagtca tccatcatgc cgggagccgg tgctttcttc aactgccgtt
      721 ggggttccac tgactggcga acagcatcaa atccagcata tccgtcacac catcgccatt
      781 gaggtctgtc tgtgcatctc cggtcgtatt gagcattttc agcaagacca tcaaatctct
      841 accgtcaatg tcaccgtcac tgtcaaaatc cgcagaacgt gtttgtggtg tcggagttgc
      901 cgttggggta ttgcttggaa cagtcacccc gctggttgtt ggtgttaccg tcggactcca
      961 tgttggggta gtgctcggtg tccgtgaggg agtgtgtgtc ggtgttcgag acggagagtt
     1021 ggttggagtt cccgtcggtg aatttgtggg tgtggctgtt ctggttggag tcacagtcgg
     1081 agtatttgtg gggctgaann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntt
     1201 gtgggtgtgg ctgttctggt tggagtcaca gtcggagtat ttgtggggct gaaggccgga
     1261 ttgtccgttg gagaattcgt aggtgttgag gacacagtcg gcgttggagt ggccgaagga
     1321 gtgcccgtag atgtcggcgt ctcagtgtgc gtgtaggtag gtgttgagga gggggtcact
     1381 gttgggctat tcgtgctggt gcgtgttggg gttgctgagg gggtatttgt ttgagtcggt
     1441 tgaccttcat ccgttggcgt tgctgtgggc gtatcggtcc ctgttggcac cgcttcatct
     1501 gtcggggttg gcgtcctgga cggccttatg aaactcgtcg gagtggacgt cggagtatcc
     1561 gttttcgttg gtttggtgat cccggtgggt gtgtccgtga atctcggaat tcgggtcggt
     1621 gtttgtgtgg gcgtagccgt tggcgtgtcc tcaggcgtgt tcgtataagt gaaggttgga
     1681 gttgccgtag gagtcaaagt ccgagtattg gttggagttc gcgttcgcgt tgcggtgcgt
     1741 gtgggtgtcg gtgtgtagct ggtcagcatg gtggacaact ggtagttgcc tttgccactg
     1801 tattcggaac ttgaaaccgc gacgacgtag gtggccgcat ttgtattctc gagcgtatag
     1861 gagacatttg ttcctacgcc gcttccgtat tgctgtgcca acatcgcacc ggtcgtatcg
     1921 ttgaacacaa tcagcattcc ctgaaaaccc acaggtgata gggaaaggct caaacgacca
     1981 ttgtaattgc caaaatgggc cgtgttcact cgatagtagt tgatgtggaa gttcgaacca
     2041 agtgcgctac tgatattggc gtatccgaga gcatccagct cgacgtctgc ggtgtactga
     2101 tttccaccat tgatcgtaag cctgtactgg gcgtttgtgc cagtcagcgc cgtttcggtg
     2161 agcagctgat aacgatgcgg tatttgtgct gcggagaaga aactcagcga tgcggcattg
     2221 cctgttccgg aatcgtagtc caaggcaaca aagtcattgg atgcttcctt aaagatgccg
     2281 atcatcggat cggatttccc atccagcgat tgcacggtga cattgacgct ggttccggag
     2341 cgaaaggccg ctcggacaat gtcaatatcc gccgtgctgt agaaatcagc ttcgtagacg
     2401 acgtctccca tgtgattggg gtacaggttg aattcatagg gttcaggacc cttggcgagc
     2461 atggctgcgc caaccgcatg aacgcgcccc catccgaacc aacgatcatg cccagacgct
     2521 ttcaagtcca cagccgaccg tctcagcagg tcatggatct gcgttgctga aagacttccg
     2581 ccggttccct tcaggtcttg gagcaaagcg gcaacggcag caacattagg tcctgcggcg
     2641 gacgtgccac agaagttcgg cagcccgtct ccatcaatat cgccaccgaa gaacgaagta
     2701 ttgacacaat caatacccat gaaatcgggc ttctgataaa cgattggcgt aggataggga
     2761 gttcctgatg ggttgaaaaa atgcgtgtga ggtccaggac aacttccgta tgtggttgcc
     2821 ttgcctgcgt tgtccggatc agtgtatggg atctcgccaa cggcaaaacc tttgcttcca
     2881 cccacgtgag ccacaatgct gcccccggga tagagtccct gatactccat gctcttgcca
     2941 ccaaagataa tcttcagcgt caagcccgct gcactcgcgc cagcggcgcg gcggatgatg
     3001 acatcaacct gggcatcact acccgtgttg ttcgtccagg aaagtgtatc gttgggattg
     3061 ccaccgatgt tgttttccgt gctctcggca atgagtgtcc catttcgaaa gaaacctatc
     3121 tgcatgtttg ttgtggcgtt tccccacggc tgcgtccact gcaagtcgca ataggcgttc
     3181 ttgctgttgg caaccacaag actgagaacc tcatctgtgc cctcaaactc atgccagtcg
     3241 tttccattgg tatctcggaa tacacgttga taggtaaaat cctgcgaatt gttgccggcg
     3301 gaaccaaagt aaacccctcc accgtctacg aagttgttaa tggcaaccgc aataacgccg
     3361 tcctgaaacc acggctcacc gccatagccc acatcatcgt tgataatatg cattccgctg
     3421 ttagccagat gggtgatgtt gttcgcaaaa tcggcttgag agtagtatgc cgtcttgaat
     3481 ccgagcgtcg ttacaccggg agccatgtca tagatcaact ccatcatggc gcgcccctca
     3541 tccgagcgca ctccactcgg atcgcccaag aacagaatgc ggttgtcgg
//
LOCUS       DAOQUC010000235         3584 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_29598_length_3584_cov_3.371777, whole genome shotgun sequence.
ACCESSION   DAOQUC010000235 DAOQUC010000000
VERSION     DAOQUC010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3584
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29598_length_3584_cov_3.371777"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="PKH07_05275"
     CDS             complement(<1..192)
                     /locus_tag="PKH07_05275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34394.1"
                     /translation="MTQKGSIDEMRSETIWLIVLILVAHVGMGFASPLGKPGTVASYE
                     LNADLADRPEYERERQLTVI"
     gene            complement(265..2679)
                     /locus_tag="PKH07_05280"
     CDS             complement(265..2679)
                     /locus_tag="PKH07_05280"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNT34395.1"
                     /translation="MACDTIGLLVSELEVCVRVRKSRKKGKAFLNLSVSESQAVFDGL
                     AELVVRQDRQHCVLWANQAACSSMGIAKDEIRGRHCYEIWRGSSEPCVGCPVTKAMET
                     GIPCESTLKAPDGRWLFIRGFPLRDNQGEIIGALEIADDVTDIKTAEMSLARRDTFIE
                     TVLDHLPVGVIVNKASTGENLFMNSAYVQMMGWSKEILLDPEKSFHCLFPDFDYRNRF
                     VDQLKTGIKTGDPEKMHWEDVMVTTGAGASLFVTIQGFLIPEQDTICFTITDMTKRFH
                     DRVTLKRSEDRFRVISELVSDHAYGLRVRENGVTVWKWGTGNRDTIFGTPLKERDFLK
                     QWKNYVHPEDAELLNRHLEKIVTGQADEAEFRIIRPSGEVRWLINQAHAWGEKEYGPG
                     NYIIGALRDVTEARLAEQEQRKMEARIQQSQKLESLGILAGGIAHDFNNLLMAILGNA
                     DLALTDLSTASPARPYLESIDQASRRAASLCQQMLAYSGKGRFVIETLNLNEVLREMT
                     HMLEVSISKKAVLKFNMDENLPSVEADATQIRQILMNLITNASEALEEQSGVIAISTS
                     AVVCSQDDLAGISLDEHLPEGLYVCIEVSDTGCGMNATTQHKMFDPFFTTKFTGRGLG
                     MAAVLGIVRGHKGAIKVTSTVGEGTTIRVLLPAREDLISPVGQPDSKRTEDWIGSGTL
                     LIAEDEEHVRTLTKSMLERLGFTVLAASNGLEAVDIYRSKRKDIVCVLLDLTMPGMDG
                     EETFHELRKINKAVRVVITSGYNEQRVTEQFAGKGLAGFIQKPYTLRSLRESLQKVLR
                     AGSSRI"
     gene            complement(2731..>3584)
                     /locus_tag="PKH07_05285"
     CDS             complement(2731..>3584)
                     /locus_tag="PKH07_05285"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNT34396.1"
                     /translation="FFAAQAALAQRKAPQHTRFLFYTWENIYRERTYASRISFVYRSI
                     EKRCYEAFHGAICATESAHQVLRRKGFTKPAAVIPYGIDSSFAATASIPKAEARAMLH
                     LPDDFLVGYVGRLLSMKGIDVLMSAFSRFNQGLLLIVGTGEEERNLRSLIASLGIGDR
                     VRLVGSVPHEQVPLYMRALDVLVLPSRTTPLWQEQLGRVLLEAMATETVVVGSSSGAI
                     PEVIGDAGLVFQEDNVEQLSEMLTRLQEQSSLMDEMRVRGVRRVLDRFTWKRFAQDMA
                     AFWESLR"
BASE COUNT          827 a         1014 c          902 g          841 t
ORIGIN      
        1 aatgacagtc agctggcgct cccgttcata ctcagggcga tctgctaaat cggcattcaa
       61 ctcgtatgag gcaacggttc cgggcttgcc caacggagag gcgaacccca ttcccacatg
      121 ggccaccaag atcagaacga tcaaccagat agtctctgag cgcatttcat caatcgatcc
      181 tttctgtgtc attttgggtc tgccaggcta ttggcgatta acatactgaa agacagtcac
      241 ttgtggtgcg tggtatctct cagttcagat tcttgaagag cccgcgcgca ggactttttg
      301 aagactctct cgcaaagagc gaagggtgta gggcttctgg atgaaacctg ccagtccctt
      361 gccagcaaac tgctcggtga ctctctgttc attgtagcca ctggtgatca cgacacgcac
      421 cgccttgttg atctttcgca actcgtggaa tgtttcctca ccatccatcc cgggcatcgt
      481 caaatccagg agtacgcata caatatcctt gcgcttgctt cgatagatat ccaccgcttc
      541 aagaccgttg ctggctgcaa gaaccgtaaa gccaaggcgc tcgagcatgc tcttagtcag
      601 cgtccgaaca tgttcttcat cctccgcaat cagaagagtc cctgacccga tccagtcctc
      661 tgtgcgcttt gaatcgggct gtccgacagg cgaaatcaag tcttctcgag cgggtaacag
      721 gacgcgaatt gtcgtgccct cgccgacggt acttgtaacc ttgatggcac ccttgtggcc
      781 acgaacgatt ccgagcacgg cggccatgcc aagtccgcgt ccagtaaact tggtcgtaaa
      841 gaagggatcg aacatcttgt gttgcgttgt agcgttcatc ccgcagccgg tgtcgctcac
      901 ctcgatgcag acgtagagtc cttccggaag gtgctcatcc agagagatgc ccgcgagatc
      961 atcctggctg cacacgacgg cactggtgct gatggcgatg acgccgctct gttcttccag
     1021 cgcctcggaa gcattggtga tcaggttcat gaggatctga cgaatctggg tagcatccgc
     1081 ctcgacggat ggcagattct catccatatt gaacttcagc actgccttct tggaaatgga
     1141 tacttccagc atgtgcgtca tttctcgaag cacttcattg aggttcagag tctcaatgac
     1201 aaagcgtcct ttgccggaat aggccagcat ctgctgacac aaagaagctg cccgcctcga
     1261 agcctggtca atgctttcca gataaggccg agcgggtgag gcggtagaga gatcggtcag
     1321 tgccaggtcg gcattgccaa gaatggccat cagcagatta ttgaagtcat gagcgattcc
     1381 accggcaaga atgccgagac tttcgagctt ctggctctgc tgaattcggg cttccatctt
     1441 tcgctgctcc tgctccgcca gacgagcctc agtaacatcg cggagtgcac caatgatgta
     1501 gtttccaggc ccgtattcct tttcgcccca agcgtgcgcc tggttgatca accagcgcac
     1561 ctcgcccgaa ggacggataa tccgaaactc ggcctcgtcc gcctggccgg ttacgatctt
     1621 ctccaggtgc ctgttcaaca actcggcatc ttcaggatgc acatagtttt tccattgctt
     1681 cagaaaatct cgttccttta ggggagttcc gaagatcgtg tctcggttgc cggttcccca
     1741 tttccaaacg gtcactccat tctcccgaac cctcaggcca taggcatggt cagaaacaag
     1801 ttcagagatg actcggaacc gatcttcact tcgcttgagc gtaacgcgat catgaaatcg
     1861 cttggtcatg tccgttatgg tgaagcagat tgtgtcctgc tccgggatga ggaatccttg
     1921 gatcgtaacg aagagtgatg cacctgcccc tgtcgtaacc atgacatctt cccaatgcat
     1981 tttctcggga tccccagtct taatccctgt cttgagttgg tcgacaaagc ggtttctgta
     2041 gtcgaaatcg ggaaagaggc agtggaagct cttctcagga tccaggagta tctccttaga
     2101 ccaacccatc atctgaacat aggcgctgtt catgaagagg ttctccccag ttgaggcctt
     2161 gttgacaatc actccaacgg gaagatgatc cagcactgtc tcaataaaag tgtcgcgtcg
     2221 ggcaagagac atctcggcag tcttgatgtc ggtgacatcg tcagcgatct ccaaagcgcc
     2281 gatgatctcc ccctgattgt ctcgcaaggg gaagccgcgg atgaacaacc acctgccgtc
     2341 cggtgccttc aaagtacttt cgcaggggat tcctgtttcc atggctttgg tcacagggca
     2401 accgacacag ggttcactgc ttccacgcca tatctcgtag cagtgacgtc cacgtatctc
     2461 atccttcgca atgcccatag acgaacaagc agcctgattc gcccaaagga cgcagtgttg
     2521 tctgtcttgg cgaacgacaa gttcagcaag cccgtcaaag acggcctggg attccgagac
     2581 actcaggttg agaaatgcct ttcccttctt gcggctcttt cggactctca cacagacctc
     2641 caactcactg accagaaggc cgatcgtatc gcacgccata tcctacgcaa agccattata
     2701 gacaaaccga gtttcgtatg gcaacagcgt tcagcgcaga ctttcccaaa atgcagccat
     2761 atcctgggca aagcgcttcc atgtgaaacg gtcgagaacc ctcctgacac cccgcactct
     2821 catttcatcc atcaacgaag attgctcctg gagtcttgtg agcatttcgc tgagttgttc
     2881 cacattgtct tcctgaaaaa cgagtcctgc gtcaccaatg acttccggaa tggctccgga
     2941 agaagagcca acgacgaccg tctctgttgc catcgcctcc agaagcactc tccctaactg
     3001 ctcctgccac agtggagtcg ttcgggaagg caacacaagc acatccaatg cgcgcatata
     3061 taagggcact tgctcatggg gaacagaacc gacaaggcgc acccgatctc cgatgccaag
     3121 tgaggcaatc agactcctca gattccgttc ctcttctccg gtgccaacga tgagaagcag
     3181 tccctgattg aaacgggaga atgcgctcat gagaacgtct atccccttca tggagagcag
     3241 gcgaccgaca taaccaacaa ggaaatcgtc cggcaggtgg agcatggcac gtgcttctgc
     3301 tttcggaatg cttgctgtgg cagcaaatga ggaatcaatg ccgtaaggga tcacggccgc
     3361 aggtttggtg aatccctttc tccggagaac ctgatgtgct gactccgtcg cacagatcgc
     3421 tccatggaat gcttcgtagc agcgtttctc gatacttcgg tagacaaagg aaatacggga
     3481 agcataggtg cgctctcgat agatgttctc ccacgtgtag aacagaaatc tggtgtgctg
     3541 tggggctttt cgttgcgcca gggcagcctg tgcggcaaag aaac
//
LOCUS       DAOQUC010000236         3566 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_29811_length_3566_cov_3.505839, whole genome shotgun sequence.
ACCESSION   DAOQUC010000236 DAOQUC010000000
VERSION     DAOQUC010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3566
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29811_length_3566_cov_3.505839"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1991
                     /locus_tag="PKH07_05290"
     CDS             <1..1991
                     /locus_tag="PKH07_05290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34397.1"
                     /translation="NVRVQQKSIAVPLMRSIFDENLAGDDTCVCLVRFRFQNTGSVAE
                     EVRLPIGYSSESGRSAPSLRPRHDRADKLFVRDNRIESLVDGKPVLRCSFETAMKPET
                     HGDVVVMTEALQPGQETELLLKIPFIALESLEELNELDMLDFDACYEDVCDFWRQEEA
                     KGAQLKTPVEPLNALHEAHVSHVQITDFRMPDEPSLINTSVGTSTYGNFSNESCMIVQ
                     ELDQRGLHEDARRRLELWLKYQGTAEQPGNFTDYDGMFFGAGGFEQGAYNQHHGWVLW
                     CLCEHLFLTGDRTWFESIADKVIAGADWVFRQRRNTMKPLSHSRGWEYGFLPAGSLED
                     VTDFHYWLSTNSLTWRGTEWAACALEHVGHPEASRVREEANAYREDLVHGFETMRRHS
                     PLVRLRDGRWIPQYPSRLYCRGRDTGWIRQLLEGSVYLLISGLYDSKSVEASWILDDY
                     QDNLYVQPPYGYLIPNFEANWYHRGGFSIQPNLLAGLLPYLERDEPEVYIWMFFNAWV
                     ACYREDINAMIEHPAPVLGYSNAAHFKTSDEANAVMWLRYMFVYSSGESLYLGRAIPR
                     GWFAAEQHFELKGVSTPFGEVGVRYEPHLAQSKIRAFADLTFARKPKRILLRFRHPEN
                     KPVRSVSVDGKKHRRFNSETGDVDLTGFEGKVAVEATY"
     gene            complement(2059..>3566)
                     /locus_tag="PKH07_05295"
     CDS             complement(2059..>3566)
                     /locus_tag="PKH07_05295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34398.1"
                     /translation="ALVVHKQWLYHEHLPYPIAGFTKSLFPPQQGGRSSLFESYPFWI
                     GLSLVFLVNLNNYLCQWFPNQLIPFRLQFDLGSLTQLFPTFQRGGGLDLMAGCCRFYF
                     TVVGMAYFLAADVSFSLGISPFVYCVVAGFFAGYGIPLVAGAFLHPMPIRFICFGSYL
                     GVFLTVLYTGRHYYAHVFREALKFHPVESDSAVAIWGLRILVVSLVAFALQLVFVGLD
                     WPLAILYTLATAIFFFIMGRVIAETGLFFFQPFWCPSVIILGVFGAKALGPQSVLIMS
                     LLCTIFMIDPREVLIPYVVNSFGLVDQLKMKISKVAQWSVVSLLVGLTVAVPVMLYFQ
                     YDRGVNSKDQWAVAWVPRFAFDEAVWVKQRLIAQDSLQTTNSLGGLSRLVEIAPDRGM
                     VLWGLAGLTAVLLLSACRLRFPRWPIHPILFLVWYSYAGKMFAWSFLLGWALKVMATK
                     YGGAAGYQKIKPFMIGIIAGDMLGAFVPFLYGLIHYFVTGDIPPAFAILPA"
BASE COUNT          912 a          906 c          981 g          767 t
ORIGIN      
        1 ggaatgtgcg tgttcagcag aagagcattg cggttccctt gatgcgttcg atttttgacg
       61 aaaaccttgc tggtgatgac acgtgtgtct gtttggttcg atttcgtttt cagaacaccg
      121 gttccgtcgc cgaggaagtg cggttgccca tcggatactc atccgaatcg gggcgttccg
      181 ctccatccct gagaccgcgt cacgatcgcg ctgacaagtt gttcgtgaga gataaccgca
      241 tcgaatcttt ggtggatgga aaaccagtgc ttcgatgttc atttgagacc gcaatgaaac
      301 cggagacaca tggtgacgtc gtcgtcatga cggaggcgct tcagcctggt caggaaacgg
      361 aactgctcct gaagattcct ttcatcgcgt tggaaagcct tgaggaactg aatgagttgg
      421 acatgcttga cttcgatgcc tgctatgaag acgtctgtga cttctggaga caggaagaag
      481 caaagggcgc gcaactcaag acgccggtgg agccgctcaa cgctctgcac gaggcgcatg
      541 tgtcccacgt tcaaatcacg gacttccgaa tgcctgacga accgagtctc atcaatactt
      601 cggttgggac ttcgacatac ggaaacttca gcaatgagtc ctgcatgatc gttcaggaac
      661 tcgatcagag gggattgcat gaagacgcca ggagacgtct cgaactgtgg ttgaaatatc
      721 aaggaacagc agagcagcct gggaatttca ccgactacga tgggatgttt tttggtgcag
      781 gtggattcga gcagggtgcc tataaccagc atcatggatg ggtgttgtgg tgtctctgtg
      841 agcacctttt cctgacgggt gaccgcacct ggttcgaatc tatcgccgac aaggttattg
      901 ctggtgcgga ctgggtcttt cgtcagcgcc ggaatacaat gaaaccgctg tcgcactcac
      961 gtggatggga atacggattt ctgcctgcgg gcagccttga agacgtcaca gatttccact
     1021 attggctttc cacgaattct ctgacctggc gtggaaccga gtgggcggct tgtgccttgg
     1081 agcacgtcgg gcatcctgag gcttcgcggg ttcgcgaaga ggcgaacgct taccgagaag
     1141 acctggtgca tggtttcgaa acgatgcgaa ggcactcgcc gctggttcgg ttgcgagatg
     1201 ggagatggat tcctcagtat cccagccgtt tgtattgccg cggccgagac accggttgga
     1261 ttcggcaact cttggaagga tcggtctatc tgctcatttc ggggttgtac gattcgaaat
     1321 ccgtcgaggc atcctggata cttgatgact accaggacaa cctttacgtt cagccccctt
     1381 acggttactt gatcccgaat ttcgaagcca attggtatca tcgaggtggt ttttcaattc
     1441 agccgaatct gctcgccggt ctcctgcctt atctcgagcg ggatgagccg gaagtctaca
     1501 tctggatgtt tttcaatgcc tgggtggcgt gctatcgcga ggatatcaac gcgatgatcg
     1561 agcatcctgc ccctgtactc ggatactcca atgcagcaca tttcaagacc agcgacgagg
     1621 ccaatgcggt catgtggctt cggtacatgt tcgtttactc ctcaggcgaa agcctttacc
     1681 tcgggcgtgc aatcccgcgt gggtggttcg ctgcagaaca acactttgaa ctcaaagggg
     1741 taagtactcc ctttggtgaa gttggcgttc gatacgagcc gcatcttgca cagtccaaaa
     1801 tcagggcgtt cgcggatttg acttttgccc gcaaaccgaa acgaattctt ctgcgatttc
     1861 ggcaccctga aaacaaaccg gtgcgaagtg tttcggttga cgggaagaag cacagacgtt
     1921 tcaactccga aacgggagac gttgatctaa ccggctttga aggcaaggtg gctgttgaag
     1981 ccacttactg atcaagggac aacgatcaga ctgggcaaca gatgatcttc tcgaggtatt
     2041 ggccttttca aaccatgtct acgccggcag gatggcgaag gcagggggaa tgtctccggt
     2101 gacaaagtag tggatcagac cgtagaggaa agggacgaat gcgccgagca tgtcgccggc
     2161 gatgatgcct atcataaagg gtttgatctt ctgatagccg gccgcccctc catatttggt
     2221 tgccatgact ttgagtgccc atccgagaag aaatgaccaa gcgaacattt ttcccgcata
     2281 ggaataccag accaaaaaca agatggggtg aattggccat cgagggaatc ggaggcgaca
     2341 agcggaaagc agaagcactg ctgtcagtcc ggccagaccc cacagcacca tgcctcgatc
     2401 gggagcgatc tctacaagtc gtgatagtcc tccgagcgaa ttggttgtct gaaggctgtc
     2461 ctgggcaatc agcctttgtt tgacccagac cgcctcatca aaagcgaatc ggggaaccca
     2521 ggcgaccgcc cactgatcct tgctgttgac gccacggtcg tattggaaat acagcatgac
     2581 cggcaccgca acggtcagcc caaccagaag tgacacaact gaccattgag ccaccttgct
     2641 gatcttcatt ttcagttgat ctaccagtcc gaatgaattg accacatagg gaatgagaac
     2701 ttccctcgga tcgatcatga aaatggtaca cagcaaagac attatcagca cactttgagg
     2761 ccccagtgcc ttggctccaa acactcccag gatgatcaca gacggacacc agaaaggttg
     2821 gaagaaaaac aaacctgtct cagcgatgac tcgtcccatg atgaaaaaga agatcgcggt
     2881 tgccaaggtg tagagaatgg ccagcggcca gtcgagaccc acgaaaacca attgcagtgc
     2941 gaacgccacg agcgacacaa caaggatccg cagcccccaa atcgcgacgg cagagtctga
     3001 ttccacggga tgaaacttca atgcctctcg aaacacgtga gcatagtaat gccgtcctgt
     3061 gtacagcacg gtcaggaaca ctcccagata cgaaccgaaa cagatgaacc gaataggcat
     3121 cgggtgcaga aacgcaccgg caacgagtgg aatgccatat ccggcaaaga aacccgcgac
     3181 gacacaatag acgaaggggg aaatcccaag ggaaaacgaa acatctgccg ccaggaaata
     3241 ggccattccc acgacagtga aatagaaccg gcagcatcct gccatcaaat ccaacccacc
     3301 gcctctctga aatgtgggaa acagctgtgt cagtgaaccc agatcaaact ggagccgaaa
     3361 cgggatcaac tgattgggga accattggca gagataattg ttcagattga ccaggaaaac
     3421 aaggctcaaa ccgatccaga atggatacga ctcgaagagc gaagaacgcc cgccctgttg
     3481 tgggggaaac aaggacttcg tgaatcccgc gattggatag gggagatgtt catggtagag
     3541 ccactgtttg tgaaccacca gagcca
//
LOCUS       DAOQUC010000237         3550 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_30004_length_3550_cov_3.889270, whole genome shotgun sequence.
ACCESSION   DAOQUC010000237 DAOQUC010000000
VERSION     DAOQUC010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3550
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30004_length_3550_cov_3.889270"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2735)
                     /locus_tag="PKH07_05300"
     CDS             complement(<1..2735)
                     /locus_tag="PKH07_05300"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNT34399.1"
                     /translation="MSPKSFRLHRVHGSIAGLLAFCIIATLSVMVTGAFGEGQSGTSD
                     AAAAISQTQQHSAAQIPQGQDTAQESAATPVDPEKTFEQGLQCYQNKDFAGALQHFNS
                     VLSVDGAHSGALKYQKLAQKGLSATRQKPVVLVQESEPTVEVAQDTTPSQAQALYREG
                     VTLYKQRQYGEAKDKFTESLALVSDSRQAQQYLAKTEKAIADEEVRKTVAAQKQAEME
                     AQQRALEDAQKQESEIASMMQQGQEQLSAGDYDAALKTFAAVLERNPRHSKAVSYQKQ
                     ATTLQQKQQESLAKARETEEKTKKEEEIRSLVATGEGFMETKEFEKAIEAYQQARVLD
                     PDNYKLYSYVLKAEQAKQKTEEAEQKSEREKERQEALAQARQQEQAQQERRQALDSLI
                     SSGRANMRQGQIEQAITDLEKAQEMDPENADATTLLARARQEKDAQLQRQQEQARLEQ
                     EQQETLKAQEQEMARQAELAQQFNEALSLYQQKDFGAARERFTALLETDANYPKANRY
                     LELARAGEENERRLLASRETELEKARQREEEVARRAAIEQNYSDGLAAFKARDYEAAI
                     AHFEGVLEIQADHRGAGRYLNKAQVALEEQQLESTPTLVVSEPESTGGLLIAATEPET
                     ATVVESVQTVPEQKEERPLPVQPFIEPFTIVQSEIVVPEPSLPPAVEPPVATTQERVQ
                     ETVSEPPVPQPEAQPIEPASTTESVPAPDVGRDERIQQHLSLGKSYLEGKLFEEAKKE
                     FAAALQLDPQNSEVAGLVAQCESEIQAKQATEAAKMERAKTVTEDTAKAQAAELTQKG
                     RDEYAQGNVIKAVEYFQEAFDLDPNNTDAENFLKITKEEYAQAVEKQKTTQKQKEKDA
                     EYDKQLSQLIPSLSFVDSPIGEVLNLLATLTGLNIVAGEGVDGVVTFNIKEKTVG"
     gene            complement(2770..>3550)
                     /locus_tag="PKH07_05305"
     CDS             complement(2770..>3550)
                     /locus_tag="PKH07_05305"
                     /inference="COORDINATES: protein motif:HMM:NF015649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HNT34400.1"
                     /translation="ADARDLLTKALDRIKEVDPSHQIVASDMVTQLETTYQTTLTDIT
                     RRVPVLFTEGRSLSDQGKAQKNLETLNQALAKWEAVAKLNPEYQNIQAELRSLNEVIA
                     SLSGEQVASEAKQRIERAKQALQELEEAIKQEDFERYQKSRNDGVEHCAFIRGLTASG
                     LDALKSEAVQLERQLRQTQFPPPVDDWELVMITERPSMGKTEYQVRLKKKGSITTVDI
                     AEGDRDSASGLTVVEVDRENKAFVWVRKPDHRKTKLTLKGE"
BASE COUNT          637 a         1027 c          896 g          990 t
ORIGIN      
        1 tctcccactg tcttctcttt gatgttgaag gtcacgacgc catccacacc ttcacccgca
       61 acgatgttga gcccagtcag agttgccaac aggttcaaca cctcgccgat tggtgaatcc
      121 acaaagctca gtgaggggat cagctgagac aattgcttgt catattctgc gtccttttcc
      181 ttttgcttct gtgtcgtctt ctgtttctcg acagcctgag cgtattcttc ttttgtaatc
      241 ttgagaaagt tctcagcgtc cgtattgttc ggatctagat caaaagcttc ctggaaatac
      301 tcaacggcct tgatgacgtt accctgtgca tactcgtctc tgcctttttg tgtgagttct
      361 gcagcctgtg ccttcgccgt gtcttcggtg accgtcttgg cacgttccat ctttgcagcc
      421 tcagtggcct gcttggcttg gatttcagac tcacactgtg caaccagacc ggccacttcg
      481 gaattctggg gatccaactg cagcgccgcg gcaaactctt tctttgcctc ctcgaagagt
      541 ttgccttcga ggtaggattt gcccagactc agatgctgtt ggattcgctc atcacgccct
      601 acatcaggag cgggaacgga ttcggtcgtc gaagcgggtt cgatcggttg cgcctctggt
      661 tgcggaacag ggggttcact gaccgtctct tgaactctct cttgggtggt ggccacggga
      721 ggttcaaccg ccgggggcag tgatggttca ggaacaacga tctcggactg gacgattgtg
      781 aaaggctcaa tgaaaggttg aacggggagc ggccgttcct ctttctgttc gggtacagtc
      841 tgcaccgatt cgactacggt cgcggtttca ggttcggtgg ctgcgatcag gagaccgcct
      901 gtggactctg gttcgctcac cacaagcgtt ggagtactct cgagctgctg ctcttccaaa
      961 gccacttgcg ccttgttcag atacctgcca gcaccccgat gatccgcctg aatctcaaga
     1021 acaccctcaa aatgggctat tgccgcctcg tagtcacgcg ccttgaaggc agccaaaccg
     1081 tcagaatagt tctgctcgat ggcagcccgt cgtgcaacct cctcttcacg ctgtcgggct
     1141 ttttccagtt cggtttcgcg agaggccaga agacgacgtt cgttttcctc acccgctctg
     1201 gcgagttcaa gatatcggtt tgctttcgga tagttggcat ccgtttccag aagagccgtg
     1261 aaccgttcgc gggcagcgcc aaaatccttc tgctgataca aagacaaagc ttcattgaac
     1321 tgctgtgcca attcggcttg ccttgccatt tcctgctctt gggcctttaa ggtttcctgc
     1381 tgctcttgct ccagacgcgc ttgttcctgt tgacgctgca actgcgcatc cttttcctgc
     1441 ctggcacgag ccagcaacgt tgtggcatcg gcgttctcag gatccatttc ctgcgccttc
     1501 tccaagtccg tgatggcctg ttcgatctgc ccttggcgca tatttgccct gccggaggag
     1561 ataagggaat ccagtgcctg ccgtctctcc tgctgagcct gttcctgctg acgtgcctga
     1621 gccagcgctt cctgcctttc tttttcgcgc tcagatttct gttcggcttc ctcggttttc
     1681 tgtttggcct gttcagcttt cagaacataa ctgtagagtt tgtagttgtc cggatcgagg
     1741 actcgggctt gttgataggc ttcaatcgcc ttctcgaatt ccttggtctc cataaagcct
     1801 tcacccgtgg caacaaggct tcgaatctct tcttctttct tggtcttctc ttccgtttcc
     1861 cgcgcttttg ccaggctttc ctgttgtttc tgctgaagag ttgtagcctg cttctgatag
     1921 gagactgctt tggaatgtcg gggatttctt tccagcactg ctgcaaaagt cttcagagcg
     1981 gcgtcgtaat cgccagcact taactgctcc tgcccctgct gcatcatgga agcgatctcg
     2041 gactcctgct tttgagcgtc ttccagcgct cgttgctggg cttccatctc agcctgtttc
     2101 tgggcagcca cggtcttgcg aacttcctca tcagcgatgg ccttttctgt tttggccaga
     2161 tactgctgcg cttgcctgga gtcagagaca agggcaagcg attcggtgaa tttgtccttg
     2221 gcttcgccgt attggcgctg tttgtaaagg gtgactccct cgcggtagag tgcttgtgcc
     2281 tgagacgggg ttgtgtcctg tgccacttcc acagtcggtt cgctttcctg aaccaaaaca
     2341 accggcttct gacgagtggc cgagagacct ttttgcgcca gcttctggta cttgagagcg
     2401 cccgaatgcg caccgtccac agacaatacg ctgttgaagt gctgaagcgc acctgcgaag
     2461 tccttattct ggtagcattg caaaccctgt tcaaaggtct tctcaggatc tacaggggtc
     2521 gcggccgatt cctgggcagt gtcctgtccc tgagggatct gtgcggcaga atgctgctgc
     2581 gtctgagaga tggcagctgc ggcatctgat gtaccggact gcccttcccc aaatgcgccg
     2641 gttaccatca ccgacagagt ggcaataatg cagaacgcca gcagcccagc aatgcttcca
     2701 tgaacacgat ggagcctgaa gctctttgga ctcatctact cgatcctccg acaatctaga
     2761 attctgattc tattcgcctt tcaaggtcaa ctttgtcttg cggtgatctg gttttctcac
     2821 ccagacaaag gccttgtttt ctcgatctac ttccaccaca gtcaacccac tcgcagaatc
     2881 tcgatctcct tccgcaatgt caaccgtagt gatcgacccc ttcttcttca gccgaacctg
     2941 gtattccgtt ttccccatgg agggtctctc ggtaatcatg accaactccc agtcgtccac
     3001 gggcggtgga aactgcgtct ggcgcagttg ccgttccagc tgtacggctt cactcttgag
     3061 cgcatccaag cccgatgccg tcaaacctcg gatgaaggca caatgctcca ccccatcgtt
     3121 gcgggatttc tgatagcgct cgaaatcctc ttgtttgatg gcttcttcaa gttcctgaag
     3181 cgcctgtttg gcacgctcaa ttctctgttt ggcttccgag gcaacctgct cgccggacaa
     3241 cgaggcaatg acttcattga gtgaacgcaa ctcggcctgg atattctggt attcaggatt
     3301 gagctttgcc actgcttccc acttggccaa cgcctgattg agtgtttcaa gattcttctg
     3361 cgccttgccc tggtcgctga gacttcggcc ttccgtaaac agcacgggca cccgacgcgt
     3421 gatatccgtc agagtcgtct ggtacgtggt ctccagctga gtcaccatgt ccgacgccac
     3481 gatctgatgg cttggatcca cctctttgat ccgatccaga gctttggtca acaagtcccg
     3541 tgcatctgcg
//
LOCUS       DAOQUC010000238         3545 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_30061_length_3545_cov_2.757880, whole genome shotgun sequence.
ACCESSION   DAOQUC010000238 DAOQUC010000000
VERSION     DAOQUC010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3545
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30061_length_3545_cov_2.757880"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..423
                     /locus_tag="PKH07_05310"
     CDS             <1..423
                     /locus_tag="PKH07_05310"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012525545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HNT34401.1"
                     /translation="GIPFVGRAGQLLTRMIEGGMGIPRSSVYICNVCKCRPPENRTPE
                     PEEMIACEPFLKQQLAIIRPEVIIAMGNVAVASLLRSKQGITRLRGKFVDYEGISLMP
                     TYHPAYLLRNPALKVESWQDLQKVMSLLGIPMPERNKG"
     gene            464..1990
                     /gene="guaA"
                     /locus_tag="PKH07_05315"
     CDS             464..1990
                     /gene="guaA"
                     /locus_tag="PKH07_05315"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HNT34402.1"
                     /translation="MNQIVIIDFGSQYSHLIARRIRESQVYAQLEPPDISPERLKAIN
                     PRGIILSGGPASVYGDGAPHCSPEIFRLGIPVLGVCYGMQLMSHLLDGKVVRSERREY
                     GHALLDFGDNKLVRGLVSPQQVWMSHGDVVEQVPSGFCLVGKTKDGLAGIIVNEAQRL
                     YGIQFHPEVVHTENGITIIRNFLFDICGCTPDWTMDDYHQRITEEIRRTVGDRSVVCG
                     ISGGVDSTVLGKLLHEAVGERLRCIFVDNGFLRKNEGEQVYKRLKAGLGLPVELVDAS
                     EQFLSALQGVDDPEEKRRRIGQIFVDVFFKTAGNFDFLAQGTLYPDVIESVSTRGPSA
                     TIKTHHNRVQAILDLIAQGRVLEPLRELYKDEVRILGERMGLPKEMLWRQPFPGPGLA
                     IRILGPVCREYLGRLREADHILVDEIKRAGLYEKLWQSFAVFLPVRSVGVMGDERTYE
                     QVIAIRAVESKDGMTADWVRLPYEILGRVSNRIINEVKGINRVVYDLSSKPPSTIEWE
                     "
     gene            1998..3476
                     /gene="guaB"
                     /locus_tag="PKH07_05320"
     CDS             1998..3476
                     /gene="guaB"
                     /locus_tag="PKH07_05320"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HNT34403.1"
                     /translation="MEKLSRERFVEDIGLTYDDVLLVPGESSILPNQVDTSVQLAGNV
                     QLNVPILSAAMDTVTESRMAIALAREGGVGIIHKNMDPKEQASHVLKVKKSESGMITN
                     PLTLSPEASVADALDLMSQYHISGIPITKNDKLVGILTNRDLRFVENLEQPVSNLMTK
                     ENLITAQIGTTLEQAREILQKHRIEKLPLVDKDFRLQGLITVKDIVKAIEFPQAAKDN
                     RRRLLVGAAVSAGTEMARDELLVEAGVDLLVVDTAHGHSENVRKKVVLLKQAFPDVAV
                     VAGNVATKEGVEYLAEAGADVVKIGVGPGSICTTRVVAGIGVPQISAIFDSARALKKY
                     PHVKLIADGGIKLSGDIPKAIAAGAHAVMLGNLLAGVDESPGELVHFKGRSFKVYRGM
                     GSIEAMRGGSADRYGQKDVDDAKLVPEGIEGRVAYKGCLSGYIFQLVGGLRSGMGYCG
                     ARDLETLRAQAKFLRITSAGMRESHPHDITITREAPNYQRGN"
BASE COUNT          817 a          848 c         1047 g          833 t
ORIGIN      
        1 ggaatccctt ttgttggacg tgccggtcag ctgctcaccc gtatgatcga agggggaatg
       61 ggcattccga gatccagtgt ttatatctgc aatgtctgca agtgtcgtcc accggagaac
      121 cggacaccgg aacccgagga gatgatcgcc tgcgaacctt tcctgaagca gcaactggcg
      181 atcatcaggc cggaagtcat catcgccatg gggaacgtgg ctgtggcgtc tttgctgcgt
      241 tcgaaacagg gcattacgcg gcttcggggt aaatttgtag actacgaagg aatttccttg
      301 atgccgacat atcatcccgc ctatcttctc agaaatccgg ctttgaaggt cgaatcctgg
      361 caggatcttc aaaaggtcat gagtcttctg gggattccca tgccggagag gaacaaaggt
      421 tgatcggaag tctgaacgat acttggctga actcgacaaa gcaatgaatc agatagtcat
      481 tattgatttt ggctcacagt acagtcattt gattgccaga cgcattcggg aaagtcaggt
      541 ctatgcccag ctggagccac cagacatatc acccgaacgg ctgaaggcca tcaatccacg
      601 cggaataatc ctttcgggtg ggcctgccag tgtctatggc gatggagcgc ctcactgcag
      661 tcccgagata tttcgtcttg gcattccggt tttgggagtg tgctacggca tgcaactgat
      721 gagccatcta ctcgacggca aggttgtccg ctctgagcgt cgagaatatg gacacgcgct
      781 cctggatttt ggtgacaaca aactcgtgcg agggctcgtt tcacctcagc aggtctggat
      841 gagccatggc gatgttgtcg agcaggtgcc gagcggtttc tgtctggttg gcaagacaaa
      901 ggacgggctt gcagggatta tcgtcaacga agcacaaaga ctctatggga ttcagtttca
      961 tcccgaagtt gtccacaccg agaacggcat tacgattatc aggaactttc ttttcgacat
     1021 ctgtggctgc actccggact ggacgatgga tgactatcac caacgaatca ctgaagagat
     1081 ccgccgaacg gtaggagacc gaagcgtggt ttgtggaatt tcgggcggtg ttgattcaac
     1141 ggttcttggg aaactgcttc atgaggctgt cggagaaaga cttcggtgca tatttgttga
     1201 caacggtttc ctgaggaaaa atgaaggcga gcaggtctac aaacggctga aagcgggtct
     1261 aggtctgcct gttgaacttg tggacgcctc ggagcaattt ctctctgccc tgcagggagt
     1321 ggatgatcca gaggaaaagc gccgaagaat tggtcagatc tttgttgatg tgttcttcaa
     1381 gactgccggg aatttcgatt ttcttgcaca gggcactctc tatcctgatg tcattgaatc
     1441 cgtgtccaca agaggccctt cggcaacgat caaaacgcat cataaccgtg tgcaggcaat
     1501 tctggatctg attgctcaag gacgtgtgtt ggagccattg cgcgagttgt acaaggatga
     1561 ggttcgcatt ctgggggagc ggatggggct tcccaaggag atgctctggc gacagccgtt
     1621 ccccggtccg gggctggcaa ttcggattct aggacctgtc tgcagggagt atcttggcag
     1681 gctgcgtgag gccgatcata ttctcgttga tgagatcaaa cgggctggac tttatgagaa
     1741 actttggcag tcctttgctg tgtttttgcc tgttcgttcg gttggagtga tgggtgacga
     1801 aagaacctat gagcaggtga ttgccattcg agcggtggag agcaaggatg gaatgactgc
     1861 agactgggtt cggctgcctt acgagattct tggccgtgtt tccaaccgca tcatcaatga
     1921 agtcaaaggc attaaccgtg ttgtctacga tctgagttcc aagccaccga gcacaataga
     1981 atgggagtag agcatctatg gagaaattga gtcgagagag attcgttgaa gacattggcc
     2041 tgacctacga cgacgtcctg ctggttccag gtgagtcctc catcctgccg aatcaagtgg
     2101 atacctcggt gcagctggct ggaaatgtcc agttgaacgt gccgatcctc agcgctgcca
     2161 tggacacggt gacagagtcc cgaatggcca tcgcactcgc tcgcgaaggc ggggtcggga
     2221 tcatacacaa gaatatggat ccgaaagagc aggcctctca tgttctgaag gtcaagaagt
     2281 cggagagcgg gatgatcacc aatcctctga ctctctctcc agaagccagc gttgctgatg
     2341 ctctggatct gatgtcgcaa taccacattt caggcatccc catcacgaag aacgacaagt
     2401 tggtgggcat tctgaccaac agggacctgc gttttgtcga gaatctggag cagcctgtca
     2461 gcaacctgat gaccaaagag aacctcatca cggcgcagat tggtaccacg ttggagcagg
     2521 ctcgggaaat ccttcagaag caccgcatcg aaaaactccc tctggttgat aaggatttcc
     2581 gtcttcaggg tttgattacg gtgaaggaca tcgtgaaagc gatagagttt ccccaagcgg
     2641 ccaaggacaa ccgtcgtcgg ctgcttgtgg gtgcggccgt gagtgctggt acggagatgg
     2701 cgcgcgatga gctgctcgtc gaggcgggtg tggacttgct ggttgtggat acggcgcatg
     2761 ggcactcgga aaacgttcgc aagaaggtcg tcctgctcaa gcaggcattt ccggatgttg
     2821 cagttgttgc gggaaacgta gccaccaagg aaggggtgga gtatctggcc gaagcaggcg
     2881 cggatgtggt caagatcggt gtggggccgg gttccatctg cacgacccgt gtcgtggcgg
     2941 ggatcggagt tcctcagatc agtgccattt tcgactcggc aagggcgctc aagaagtatc
     3001 cccatgtcaa actcattgcc gacggtggca tcaaactctc tggtgacatt cccaaagcga
     3061 ttgcggccgg tgctcatgcg gtcatgcttg gcaacctgct ggcgggcgtt gacgaaagcc
     3121 cgggagaact ggttcacttc aagggacgct ctttcaaagt ctaccgtggc atgggatcca
     3181 tcgaagccat gcgtggggga agcgctgatc gctacggaca gaaggacgtt gatgacgcaa
     3241 aacttgttcc tgaaggcata gaaggtcgag ttgcctacaa aggctgtctt tcaggctaca
     3301 ttttccaact ggttggtggt ctgcggtctg ggatgggcta ctgcggggca cgggatcttg
     3361 aaacactgag ggctcaggcc aagttcctgc gcatcacgtc cgccggcatg agggaaagcc
     3421 atccgcatga cattacaatc acacgtgaag cgcccaacta ccaaagagga aattgatttt
     3481 tcatcagacc cgggttgaac ccgggtcttt tcaactaatt gtgtaacttt ttcggggttc
     3541 ctcca
//
LOCUS       DAOQUC010000239         3531 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_30239_length_3531_cov_3.512658, whole genome shotgun sequence.
ACCESSION   DAOQUC010000239 DAOQUC010000000
VERSION     DAOQUC010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3531
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30239_length_3531_cov_3.512658"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            471..1700
                     /locus_tag="PKH07_05325"
     CDS             471..1700
                     /locus_tag="PKH07_05325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34404.1"
                     /translation="MAWTITSVTGEAQIFVDGTLIYTCDKIAKGSFSFGPDGNGYAFA
                     AASGNEWRIVTHESESDLYEQVTNPVWSSESECVYAVKSASETHVFSNQRKVHSFQGI
                     LDNRILVDPETRHFIYAGGQERDWAVFVDHAPIGEGFNTVALKLAKFCGTEGFVWVPV
                     EREGEWYFICSDGREEGPFLALSGLATSSDERHLGYIASREPGRFVVVFNGKQVATHK
                     SATDLRLSEDGQHYCYMFREGKQRFVMASGTLYGPYESVIGSSLKLSPDGNSCAFVAT
                     RDGRVFIVRDGEENKPVDDVKTKTLKFSNDSRRLRYIAKWDKREFVVLDGKAGRLFTW
                     IPPNSVLFSEDNKGIAYPVLLFYKTFMMCNERRGTYQSWIYPETVSFTSDQTVSYVAY
                     SEKQIRICRERITRDFR"
     gene            1780..2301
                     /locus_tag="PKH07_05330"
     CDS             1780..2301
                     /locus_tag="PKH07_05330"
                     /inference="COORDINATES: protein motif:HMM:NF018942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030416 - methylamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MauE/DoxX family redox-associated membrane
                     protein"
                     /protein_id="HNT34405.1"
                     /translation="MFERDRKQACCSGMMECPICRGIWWLLRNPWLGLASRVFVAAIF
                     IYASFDKIAHPPQFAHSVLNYEFFPVWSVNLIAIMVPWIELIVGILLLVGLFTYASNL
                     VVFGMLCFFFVLIAQALMRGLEIDCGCFSVHLTGQIMDWTYLVRDLLFIACSVHVFTA
                     RRLVLALDRLRGA"
     gene            complement(2304..>3531)
                     /locus_tag="PKH07_05335"
     CDS             complement(2304..>3531)
                     /locus_tag="PKH07_05335"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNT34406.1"
                     /translation="PTPERACGHPRTPVGQLLWQRHVVSTEEACYRKKTPKESLDYGA
                     AIVQRELDLILHPREGKPVNWSYFFIAYAVLTLLLALGAFIWDTSMGFRRQLARLLFR
                     KREVGLGDLVEGSRGGYFRAQWWAGYLFVAPWVIGFVVFGGGPLLFSILMSFCDYDVI
                     NPAKWIGFENYRWMFSQDELIPVAFRNTIFMVVGVPLGMIASLAIALLLNLRIRGISV
                     WRTIFYLPAIVPAVAAFILWIWIFNPAGGFINQALSLIGLEGPNWLGSEIWSKPSLIL
                     MGLWTAGGGMLIWLAGLKGIPEHYYEAAAIDGASEWKQFLHITLPMLTPYIFFNLVMG
                     LIGVFQIFDAAFVMTAGGPVNSTLFYVYHLFNNAFRYGHMGYACAMAWVLFVIILGLT
                     VVQLKLAKRWVHYESE"
BASE COUNT          866 a          893 c          907 g          865 t
ORIGIN      
        1 tgctcccacg atggttgttc cgattccttc caaccacact gggcgctccg ctgattcttt
       61 ctgggctgat ttggctacta tgccgacgac atgaccagac aaggtttccc ttcactcacc
      121 tgattcgaga gatatgcgaa ctgcagctca tttggttttt atttgaatcg ctgttccagg
      181 cgcttcttat gaaacaactt ttgctgccaa agcacgtcaa cctgttacgg cctgttgtgg
      241 cttctctgtt gttcgtagct ctttcgcgaa ggttgttcct cctcaataag aggaagatcc
      301 tgaggtttcg cagtgctgtt ttgatgatgc tttccatgat ctccttcgta atactgaccc
      361 tgttgatgtg gaaagagcaa gagagcgatt ggtctataag cataggcagt tcgtatgatg
      421 taggtaattc cattcctctt atagttggct ggcacgaggg atccgagaag atggcgtgga
      481 cgatcacaag cgtcacggga gaagcgcaga tcttcgttga cggcacactc atatacacct
      541 gtgacaagat agcaaaagga tcattctcat ttggtcccga cggcaatgga tatgcttttg
      601 ctgcggcgtc tggaaatgag tggcggatcg tcacccatga aagtgagagt gatctgtacg
      661 aacaggtcac caatcccgtc tggagttcag aatctgaatg tgtctatgcg gtgaagtctg
      721 cttcggagac tcatgtcttc tccaatcaac gcaaagttca ttcattccag ggaattcttg
      781 acaaccgcat ccttgtcgat cccgaaacaa gacatttcat ctacgctggc gggcaagaga
      841 gagactgggc ggtgtttgtg gatcatgcgc caattgggga aggcttcaac accgttgcgc
      901 tcaaactggc gaagttctgc ggtacggaag ggttcgtttg ggttcccgta gaacgagagg
      961 gagagtggta tttcatatgt tcggacggca gagaagaagg acctttcctc gcattgtcgg
     1021 ggcttgccac gtcatctgat gaaagacatc tcggctatat tgcatcaaga gagcctggtc
     1081 gttttgtcgt cgtattcaat ggcaaacagg tggcaacaca caagtcagcg actgatttga
     1141 gactcagtga agatggacaa cactactgct acatgtttcg agagggcaaa cagcggtttg
     1201 tcatggcatc gggaacgctc tacggaccct atgaaagcgt cattggatcg agcctgaaac
     1261 tatcacctga tgggaacagc tgcgcttttg ttgcgacaag ggatggtcgg gttttcatcg
     1321 tgcgagatgg tgaggaaaac aaaccggttg acgatgttaa gaccaagact ttgaaattca
     1381 gcaacgacag ccgccgcctg agatatatcg ccaagtggga caagcgtgag tttgtggtgc
     1441 ttgatggcaa ggctggacga ctgtttactt ggataccccc caacagcgtt ctcttctcgg
     1501 aagacaacaa gggtatagcc taccctgtgc tcctgttcta caagactttc atgatgtgca
     1561 atgagcgaag aggaacctat cagtcttgga tctaccctga gacggtttct ttcacttcgg
     1621 accagactgt ctcctatgtc gcatattcgg agaaacagat tcgcatttgc agggaaagaa
     1681 tcacacgtga tttccggtaa ctgatatcac tgagtgtttg tgaacttgca tcgcctgccg
     1741 aagcgttcta taactatccg tcagtaagaa aggtgcatca tgttcgagcg tgacaggaaa
     1801 caggcttgtt gttcagggat gatggagtgc ccaatatgcc gtggaatctg gtggttgttg
     1861 cgaaatccct ggctgggtct ggcttctcgg gtcttcgtcg cggcgatctt catctacgcc
     1921 agtttcgaca agattgcgca tccaccgcag tttgcccact ccgtgctcaa ctatgagttc
     1981 tttcccgtct ggtctgtgaa tctcattgcc atcatggttc cttggatcga actgatcgtg
     2041 ggaattcttc tgttggttgg actcttcacc tatgccagca atctggtcgt attcggaatg
     2101 ctttgtttct tctttgttct cattgcgcag gctctgatgc gcggactgga gatagactgc
     2161 ggttgctttt ccgtccattt gacggggcag atcatggatt ggacttatct cgtgcgggat
     2221 ttgctgttca ttgcttgcag cgttcacgta ttcaccgcgc gcagactggt gcttgccctc
     2281 gacagactcc gaggggcata gaattattcc gattcgtaat gcacccagcg tttcgccaac
     2341 ttcagctgca cgacagtcaa acccagaatg atgacaaaga gaacccatgc catcgcgcag
     2401 gcatacccca tgtgcccgta tcgaaaggca ttgttgaaaa gatgatagac atagaacaac
     2461 gttgagttaa ccggcccgcc tgcagtcatg acaaaggcag cgtcgaaaat ctgaaacact
     2521 ccgatcaagc ccatcacgag gttgaaaaag atatacggag tcaacatggg aagcgtgatg
     2581 tgtaaaaact gcttccactc ggaagcccca tctatcgccg cggcctcgta gtagtgttcc
     2641 ggaatgccct tcagaccggc aagccagatc agcattccgc cccctgctgt ccacaaaccc
     2701 atcaggatca gcgatggctt actccaaatc tccgagccca gccagttggg tccctcaaga
     2761 ccgatcaaac tcagcgcctg gttgatgaaa ccgccggcag ggttaaaaat ccaaatccag
     2821 aggatgaacg ccgccacagc aggaacaatg gcgggcaagt agaaaatcgt tctccagacg
     2881 gaaatgccac gaatacgaag attcagaagc aacgcaatgg ccagtgatgc aatcatcccc
     2941 agcgggacac cgacaaccat gaagatcgtg tttcgaaacg ccacagggat cagttcatct
     3001 tgtgagaaca tccaacgata gttctcaaac ccaatccatt tggctgggtt gatgacatca
     3061 tagtcgcaga agctcatcag gatcgagaac agaagtgggc ctccgccaaa gacgacgaac
     3121 ccaataaccc agggagcgac aaacaaatat cccgcccacc attgtgctcg gaagtatcct
     3181 cccctggaac cctcgacgag atcgcctagt cccacttcac gcttgcgaaa caacagcctc
     3241 gccaactgtc gtcggaatcc catgctggta tcccagataa atgctcccag tgccagaagc
     3301 agagtcagga cggcatacgc gatgaagaag taactccagt ttactggctt accttcccgc
     3361 ggatgaagga tcagatccaa ctcgcgctgg acgattgcgg ctccgtaatc cagcgactcc
     3421 ttcggagttt tcttgcggta acaggcttct tcagtcgaaa cgacatggcg ctgccagagc
     3481 aactgaccga caggcgttcg tggatgtccg caggcgcgtt ctggcgttgg c
//
LOCUS       DAOQUC010000240         3528 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_30282_length_3528_cov_2.895767, whole genome shotgun sequence.
ACCESSION   DAOQUC010000240 DAOQUC010000000
VERSION     DAOQUC010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3528
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30282_length_3528_cov_2.895767"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..311)
                     /locus_tag="PKH07_05340"
     CDS             complement(<1..311)
                     /locus_tag="PKH07_05340"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HNT34407.1"
                     /translation="MISGCILAGGNSSRYGNYPKGLLEVRPGTSIIENAIEALLASAI
                     EDIAIVSDHRDAYVRFGYPILADLRRGLGPLAGIEAGLTYFESSSDGVLFLPCDLPGI
                     SG"
     gene            complement(317..1300)
                     /locus_tag="PKH07_05345"
     CDS             complement(317..1300)
                     /locus_tag="PKH07_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020264103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNT34408.1"
                     /translation="MKAATPQELESSFKQISSQYDVFAPIRMHDGSRSLGRLDEGPLA
                     LMGGRIFTKTTSVFFPQQECILTQEGSGVAMQQPVGKPLLVVGFTAEDADCLAFIDKF
                     YSENFRDSIYFNKRDGSVVVVVSGRCGKDGAFQKIAGRNCDLELAYDGDRFLVIPYSA
                     AGVAVGDAIKAKGSANTLAELKAQSESLDDQDQKIIEKASDLLVAEKVPEEFWRDISK
                     RCIACTACNLSCPTCTCFDVFDWQSDNKVKRLRIWDSCQLDGFMREASGHNPMGTESL
                     RTRRRIHHKLAADRTRWGHITCFLCGRCDDVCPTGIGIKSVAREMVKRYGQ"
     gene            complement(1297..3339)
                     /gene="fdhF"
                     /locus_tag="PKH07_05350"
     CDS             complement(1297..3339)
                     /gene="fdhF"
                     /locus_tag="PKH07_05350"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HNT34409.1"
                     /translation="MKRVLTTCPYCGTGCCFYLVVNDQGQLVGVEGDSEHVVTKGQLC
                     VKGWTAYQFPNHPDRLTDPMIRRNGKMVKASWDEALNLIVSRFKDIQEKYGTDSVAFF
                     SSAKTTNEENYLMNKLARAVYKTNNVDHCARLCHASTVVGLAGTFGSGAMTNSISCMD
                     EADCFFVTGSNTTEQHPLIGTRIIRRVLERGAKLIVADNRKIRLAEFADLHIRWKNGT
                     DVPFLNSIMNVIISEGLEDKEFVANRTENYEELKKVVANYPPEKAAEICGITAEEIVQ
                     ATRMFAKADKAMIVYSMGITQHTHGVDNVKTCANLVMLTGNLGKPGTGVNPLRGQNNV
                     QGACDMGALSNVFTGYQKVTDAPVREKFEKAWGVRNLPGEVGLTVTTAINAAADGKLK
                     GLFVLGENPMMSDPDQNHVREALEALDFLVVVDIFPTQTSELADVILPAACYAEKDGT
                     FTSTERRVQRVRKAVDPPGQARADWEILCDLAKRAGYDGMQYSSPSEIMDEAASVTPI
                     YGGISFDRIDQVGLQWPCPAPDHPGTPVLHVGKFTRGLGLFSPAEYIPSAELPDAEYP
                     FILSTGRSYFHFHTGTMTRRTRILDREQPGPYVEMNLEDAKELGVRDKEWVFVSTRRG
                     EVRAKALVSENIRQGVLFMVFHFEEGPANLLTINALDPVAKIPEYKVCAARVRRAS"
     gene            complement(3336..>3528)
                     /locus_tag="PKH07_05355"
     CDS             complement(3336..>3528)
                     /locus_tag="PKH07_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020264105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HNT34410.1"
                     /translation="MYLFVIMELYQKGLQNEDIIVSLERHMKCGVGKCGHCTIEHLYC
                     CLDGPVFRLDQVINLKGAL"
BASE COUNT          826 a          988 c          891 g          823 t
ORIGIN      
        1 ccgctgatac caggaagatc gcagggaagg aaaagcacgc catcgctgga tgactcgaag
       61 tatgttaagc ctgcctcaat gcctgcaagt ggccccaaac cccttcgtaa gtccgcaagg
      121 atgggatagc caaacctgac gtaagcgtct cgatgatcgg agacgatggc aatatcttcg
      181 atggctgagg ccagaagagc ctcaatagcg ttctcaatga tactcgtccc gggacggact
      241 tccagaagcc ccttgggata gttgccgtag cgcgacgagt tcccaccggc aagaatgcag
      301 cctgatatca tgcgtttcac tgcccgtatc gcttgaccat ctctcgtgca acggatttga
      361 taccaattcc cgtgggacag acgtcatcgc atcgcccgca aaggaagcac gtgatgtgtc
      421 cccatcgggt tcggtcagca gccagcttat gatggatccg tcttcgcgtc ctgagtgact
      481 cggtgcccat agggttgtga ccactggctt ctctcataaa cccgtccaac tgacaggagt
      541 cccagattct gagacgtttg actttgttat cgctctgcca gtcaaagaca tcgaaacagg
      601 tacaggtggg acaactgaga ttgcatgcgg tgcaggcgat acaacgtttg gaaatgtcac
      661 gccaaaactc ttcaggaact ttctcagcga caagaagatc ggacgccttt tcgatgatct
      721 tctgatcctg gtcatctaga gattccgact gtgctttgag ctcagccaac gtgttggctg
      781 agcccttggc tttgatggca tcgccaaccg caacacccgc tgccgagtaa ggaatgacaa
      841 gaaaacgatc gccatcatag gccagttcga ggtcgcaatt ccgaccggca atcttctgaa
      901 aggcaccgtc cttgccacaa cgaccagaaa cgacaacgac aacggaaccg tcacgcttgt
      961 tgaagtagat gctgtccctg aaattctctg agtagaactt gtcaataaat gccagacagt
     1021 ccgcatcctc tgcagtgaat ccgacgacca gaagcggttt tccgacaggc tgttgcatgg
     1081 cgacgccgct tccctcttgc gtcagaatac attcctgttg cgggaagaag acgcttgttg
     1141 tcttcgtgaa gatgcgcccg cccatcagtg ccaacggacc ttcatcaagc cgtcccagcg
     1201 agcgacttcc atcgtgcatt cgaatgggtg caaaaacatc gtattgtgaa gagatctgtt
     1261 tgaaagaact ctccaactcc tgcggggtgg ctgccttcac gatgccctcc ttactcgcgc
     1321 ggcgcagact ttatactcag gaatcttggc gactggatcc aaagcgttga tggtcaacag
     1381 atttgccgga ccttcttcaa aatggaacac cataaacaga acaccttgtc ggatgttctc
     1441 ggaaaccagc gcctttgccc tgacttctcc acgacgtgtg ctgacgaaga cccattcctt
     1501 gtcacgcaca cccagttcct tcgcatcctc aagattcatc tcaacgtagg ggcctggctg
     1561 ttctcgatca agaatccggg tgcgccgtgt catggttcca gtatggaaat ggaaatagct
     1621 ccgacctgtg ctcagaatga agggatactc ggcatccgga agctccgccg aggggatata
     1681 ctctgccgga ctaaacaacc ccagccctcg ggtgaacttc ccgacgtgga ggacaggcgt
     1741 gcccggatga tctggagcag gacagggcca ctggagcccc acttgatcaa ttcgatcaaa
     1801 agatatccca ccatagatcg gcgtcactga tgcggcttca tccatgatct cactggggct
     1861 tgagtactgc attccatcgt atccggcacg ttttgcgaga tcgcaaagaa tctcccagtc
     1921 agctcgtgcc tgcccgggag gatcaacggc cttgcgaaca cgttgcacac gccgctctgt
     1981 ggatgtgaag gtgccgtctt tctccgcgta gcaagcggcg ggcagaatca catcagcaag
     2041 ttcactggtc tgagtcggga aaatgtccac gacgaccagg aaatccaagg cctcaagcgc
     2101 ttctcggaca tggttctgat cagggtcaga catcattgga ttctcaccga gaacaaacag
     2161 acccttgagt ttcccatccg ctgcagcatt gatcgctgtc gtgacggtca atccgacttc
     2221 gccaggaaga ttccgtacac cccaggcctt ttcaaacttc tctcgaactg gagcatcggt
     2281 aaccttctgg taaccggtga agacgttcga cagcgctccc atgtcgcaag cgccttgaac
     2341 gttattctga cctctgagag gattcactcc ggttcccggc ttgccaagat tccccgtgag
     2401 catcaccagg ttcgcacaag tcttgacatt gtccacaccg tgcgtgtgct gcgtaatgcc
     2461 catggagtag acgatcatcg ctttgtccgc tttggcgaac atccgagtag cctgaacgat
     2521 ctcctcggct gtgattccgc agatctctgc agccttctcg ggcgggtagt ttgcaacgac
     2581 tttcttgagt tcttcgtagt tctccgttcg gttggccaca aactccttgt cttccagtcc
     2641 ctcactgatg atgacattca tgatgctgtt cagaaacggc acatcggttc cgttcttcca
     2701 gcgaatgtga aggtcggcaa actccgcgag acgaatcttc ctgttgtcgg caacgatgag
     2761 cttggcacct cgttccaaaa cacgtcggat gatccgagtg ccgataaggg gatgttgttc
     2821 cgttgtgttg gagccggtga caaagaagca gtcggcttca tccatgcagg agatcgagtt
     2881 ggtcatggca ccagaaccga atgttccagc gagaccgacc actgttgatg catggcacag
     2941 acgcgcacaa tgatccacat tgttggtctt gtagaccgcc cgcgccagtt tgttcatcag
     3001 ataattctct tcgttggtcg tctttgccga agagaaaaac gcgacggaat cagttccgta
     3061 cttctcctgg atgtctttga accttgagac aatcaggttc aaggcttcgt cccaagacgc
     3121 tttgaccatc ttgccgtttc gccgaatcat tggatcagtc agacggtctg ggtggttggg
     3181 aaactgatag gccgtccagc ccttgacgca gagttgtcct ttggtgacca catgttcgga
     3241 atcaccttcc acgccaacca gttgcccctg atcgttgaca acaagataga agcagcatcc
     3301 cgttccacaa taagggcatg tcgtcagaac gcgtttcata gtgccccctt cagattgatg
     3361 acttgatcga gtcggaacac aggtccatca agacagcaat agagatgctc gatggtgcag
     3421 tgtccgcact tgccaacacc gcacttcatg tgccgctcga gcgagacgat aatatcttca
     3481 ttctggagac ccttctgata gagctccatg atgacaaaca gatacatg
//
LOCUS       DAOQUC010000241         3523 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_30344_length_3523_cov_2.910611, whole genome shotgun sequence.
ACCESSION   DAOQUC010000241 DAOQUC010000000
VERSION     DAOQUC010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3523
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30344_length_3523_cov_2.910611"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            228..974
                     /locus_tag="PKH07_05360"
     CDS             228..974
                     /locus_tag="PKH07_05360"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNT34411.1"
                     /translation="MAATPAESANTTQQTLCEVIYRLARAAEYREEDTYYHLHRMSHG
                     VAELARAMGMEPDFVERLSCAAPFHDIGKVGIPDRILLKPTKLVDWEWEVMKQHTTLG
                     KRILEGSDLPVLRMGETIAYTHHERWDGMGYPRGLQGSEIPVEGQIVSLVDVFDNLSS
                     RRPYRDACSLDEAFEAIREGSGSQFSTEMVEVFLGIRDRIIQIKEQFEECGESIFLQM
                     VGRLHIVSHPTILESEPEFDDQETKRISPA"
     gene            1089..1583
                     /locus_tag="PKH07_05365"
     CDS             1089..1583
                     /locus_tag="PKH07_05365"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HNT34412.1"
                     /translation="MEEQKMVLNRTKAVSFALLLVFGVANMASCTQTAPSVIQGTTQE
                     ASASPTKWFYSYDEALKAAQKDNKPILMDVYTDWCGWCKKMDQSTFGHKTFQAEASKF
                     VLAKVDGDKNKELVQKYGVRGYPTVVFLDSKGNLLQQSVGFKEASDFVVVMKEVLQKS
                     KPKE"
     gene            1756..2808
                     /gene="galT"
                     /locus_tag="PKH07_05370"
     CDS             1756..2808
                     /gene="galT"
                     /locus_tag="PKH07_05370"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HNT34413.1"
                     /translation="MPELRKDHIIGRWVIIASERGKRPCDFKNIPESQSQAFCPFCNG
                     NEAMTPPEVLAYRHAGTKPNEAGWWVRVVSNKFPALSPDGDVVRTGEGMYDMMNGVGA
                     HEVIIETPDHDASYATYEQSQIEEILWSFRDRMMDLRRDRRFRYILLFKNHGREAGAS
                     LEHPHSQLIATPTVPKRVQEEVSGALNYYRFKERCVYCDIVKQELDTESRVVAENDSF
                     VAFEPFASRFPFETWVVPRAHEAHFENIQKHHIMDMAKVMHTVFSRIKEVLNDPPYNF
                     IIHTSPCNEPDLAHYHWHIEIMPKLTKVAGFEWGTGFYINPTPPEQAAAWLRGELADE
                     IPQIPGANIPPKKQAV"
     gene            2837..>3523
                     /locus_tag="PKH07_05375"
     CDS             2837..>3523
                     /locus_tag="PKH07_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013840254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0280 family protein"
                     /protein_id="HNT34414.1"
                     /translation="MKSRFGTKYSKRFYRDRLPDRGLVAFSVVFEETDLYILAESDLG
                     TVAEQCVRYERACLEEYLARDSGFLNDLKPRDPRPEAPELVLSMCEAARLAGVGPMAA
                     VAGAIAQSVGRKLCAQSGEVLVENGGDIFVKTVGERTIALYAGVGSPFSMCLGLRIFP
                     DQTPLGVCTSSGTVGHSLSFGKSDASVVVARDAALADATATALGNRIQSAKDIEPAFE
                     WIQTVPGISGA"
BASE COUNT          834 a          854 c         1002 g          833 t
ORIGIN      
        1 tgaaactccc gtgcatttta tcaacggctt ctggcaaacg taggcatgaa tataaccggt
       61 tcccgttcct cgcgccaata cattatcgca gcgcggaact tgatattctt gactgtgaaa
      121 caggaaccag ggtaaactca taagtgtggg atgttgatgg cagtatggag agggatgtcg
      181 ttttgtgaga ggacgtctcc tgtgaaagga ttcgggaggg cagaaacgtg gcagcaacgc
      241 cagctgaatc ggccaacacg actcaacaga cgctctgcga ggtcatctat cgtctggcac
      301 gggctgctga gtatcgggaa gaggacacct attatcacct tcatcggatg agccatggtg
      361 ttgccgagtt ggctcgtgcg atggggatgg agcctgattt tgtcgaacgc ctgtcgtgtg
      421 cggctccctt tcatgacatc ggcaaggtgg ggattccgga tcggatattg ctgaagccca
      481 ccaaacttgt tgactgggaa tgggaagtta tgaagcaaca cacgacgctg ggcaagcgta
      541 ttctcgaagg atccgacttg ccggtgctca ggatggggga gacgattgcc tacacgcacc
      601 atgaaagatg ggacggaatg ggatatccca gaggcctgca agggagtgag attccggtgg
      661 aaggtcagat cgtctcattg gtggatgtct ttgacaacct ctcctcgcgg cgtccgtatc
      721 gggacgcctg ctcactcgat gaagcctttg aagccattcg agaaggatcg ggaagtcagt
      781 tcagcaccga gatggttgag gtctttctcg gcatccgaga caggatcatc cagattaagg
      841 agcagtttga ggaatgcgga gagagcatct ttttgcagat ggttggaagg cttcatattg
      901 taagccatcc caccattctc gaatccgaac ctgaattcga cgaccaggag acaaagagga
      961 tctctcctgc ttagttcctg ctctttgcgg gaaggaatgc actctggtga ttgtgctgaa
     1021 tcgtatctcg gccatggata caaacgtttt gtgatccaag ggtttgcgtg acccatgaac
     1081 attagattat ggaggaacag aagatggtgc tgaatcggac aaaggcggtt tctttcgctt
     1141 tgctgttggt ctttggtgtc gcaaacatgg catcctgtac gcagacggct ccttccgtca
     1201 tccaggggac aacacaggaa gcctctgcct ctccaacaaa gtggttctac agctacgacg
     1261 aggctctgaa ggcagcccag aaggacaaca agcccattct gatggatgtc tacaccgact
     1321 ggtgcgggtg gtgcaagaag atggatcagt ccactttcgg gcacaaaaca ttccaagcgg
     1381 aagcgtcgaa gtttgtcctg gccaaggttg atggggataa gaacaaggaa cttgttcaaa
     1441 aatatggagt tagaggctat ccgactgtcg tgttcctgga ttccaaggga aacctgcttc
     1501 agcagtcggt gggtttcaag gaagcttcgg atttcgttgt cgttatgaaa gaggtcttgc
     1561 agaaatccaa acctaaggaa tgacagtcta ctaattgtgt ctgtaagtgc ctttatacac
     1621 aacatttagt gtttgatggc cttctggagc ctccccttcg agctggaaaa gcttgacagc
     1681 tcgtcctgaa tcatttagag gagatgtgtt ggatttgagt gttttgttag ttttagagat
     1741 gaggagagcc tgattatgcc tgaactcaga aaagatcaca ttatcggccg atgggttatc
     1801 atcgccagcg agcgaggcaa gcgcccctgc gacttcaaga acattcccga atcccagtcg
     1861 caggctttct gtcccttctg caacggcaac gaagccatga cccccccgga agttctggcc
     1921 tatcgccatg cgggaaccaa gcccaatgag gctggttggt gggtgcgtgt tgtctcaaac
     1981 aagtttccag cgctcagtcc ggatggtgat gttgtgcgaa cgggggaggg catgtatgac
     2041 atgatgaacg gtgttggggc acatgaagtc atcatcgaaa caccagacca cgatgcctcc
     2101 tacgccacct acgaacagag ccagattgag gaaatcctgt ggtcatttcg tgacaggatg
     2161 atggatcttc gtcgtgacag acgctttcga tacattcttc tcttcaagaa ccacggtcgt
     2221 gaggcaggag cctcgctgga gcatccgcat tcccagttga ttgccacgcc aacggttccc
     2281 aagcgggtac aagaggaagt gtcgggagcc ttgaactact accggttcaa ggagcgatgt
     2341 gtctactgtg acattgtcaa gcaggagctg gacaccgaaa gcagagttgt ggccgagaat
     2401 gattcctttg tcgccttcga gccgtttgct tcccggtttc ctttcgagac ctgggttgtg
     2461 ccccgggcac atgaagcgca cttcgagaat atccagaagc atcacatcat ggatatggca
     2521 aaagtgatgc atacggtgtt ctcccgtatc aaggaagtgc tcaacgaccc tccctacaat
     2581 ttcattatcc acacgtcgcc ctgcaatgag ccagatcttg cgcattatca ttggcacatc
     2641 gagatcatgc ccaaattgac gaaggtggca ggattcgaat ggggaacagg tttctatatc
     2701 aacccgacac cacccgagca agcagcagcc tggttgcggg gagagttggc ggacgagatt
     2761 ccccagattc cgggagcgaa cattcctccc aagaagcagg ctgtctgatg gcggagtggg
     2821 gtaagcgccc acaggcatga agtccaggtt cggcacgaaa tacagcaagc gattctaccg
     2881 agaccgtctt cctgatcgcg ggttggttgc tttctccgtt gtttttgagg agacagacct
     2941 ctatatcctg gccgaaagtg atcttggcac agttgccgag caatgcgttc ggtatgagcg
     3001 tgcctgtctt gaggagtacc tggcaaggga ttcaggtttc ctcaatgacc tgaaaccgcg
     3061 agatcctcgc cctgaagcac ccgaactggt cttgagcatg tgcgaagccg ctcgtctggc
     3121 cggagtggga ccgatggccg cagtcgcagg tgccatcgca cagtcggttg gcaggaaact
     3181 gtgcgctcag tcgggggaag tgctcgtcga gaacggggga gatatttttg tcaaaacagt
     3241 tggcgagcga accatcgcgc tatatgcagg cgtaggttct cctttttcga tgtgcctggg
     3301 tttgcgaata tttcccgacc agacacctct tggcgtctgc acttcatcag gaaccgttgg
     3361 ccattccctg tcattcggca aatcggacgc ttcggttgtc gttgccagag atgctgcgct
     3421 tgctgacgcg acagcaaccg ccttgggcaa tcggattcag agtgcaaaag acatcgaacc
     3481 agccttcgaa tggattcaga ctgtcccagg tatttccgga gcc
//
LOCUS       DAOQUC010000242         3521 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_30372_length_3521_cov_3.062031, whole genome shotgun sequence.
ACCESSION   DAOQUC010000242 DAOQUC010000000
VERSION     DAOQUC010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3521
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30372_length_3521_cov_3.062031"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..746)
                     /locus_tag="PKH07_05380"
     CDS             complement(<1..746)
                     /locus_tag="PKH07_05380"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF009544.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HNT34415.1"
                     /translation="MKRRIPNWLKQPLGPSPSAMKTSLVLDESGLCTVCEEAACPNRA
                     ECYGQRTATFMILGAVCTRNCRYCNVSSGTPALPDPDEPSKVAEAVSRLGLRYVVVTS
                     VTRDDLNDKGAAQFAKVIGALKGLPSKPRIEILTPDFLGSPESVARVIEARPDVWGHN
                     IEVVPRLFPFMRPGADYHLSLDLLRRVKAEAPHIVTKSSLMIGLGETVQEILEACRDL
                     RDAETDLVVIGQYLSPTSNHTPVERFYTPQ"
     gene            complement(743..1483)
                     /locus_tag="PKH07_05385"
     CDS             complement(743..1483)
                     /locus_tag="PKH07_05385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34416.1"
                     /translation="MQLKISFCEPAAATENMARDQALVHQCAEDGISAYLRWYRWSPP
                     AFSFGYGQKPEQILDLEKAESQSIPWCKRPTGGALVYHCKDLSYGFAIPRINPFEVNT
                     GDDLYGLVNDAFMKGFHRLGIPVSRPSVVPRFRDVPQDARQLCLAHFSQGDLLATVKQ
                     KDVSAAMEQKKIGGSAQRRFRHVWLQQGFFSLVPFSVPDVFRDPTMGVLMQSACIDLQ
                     SLGFPFTRVLLQQALTDAMLERFEEAGR"
     gene            complement(1493..2869)
                     /gene="lpdA"
                     /locus_tag="PKH07_05390"
     CDS             complement(1493..2869)
                     /gene="lpdA"
                     /locus_tag="PKH07_05390"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HNT34417.1"
                     /translation="MAAERYNLIVLGGGPGGYTAALTAARHKARVALVEAQAIGGTCL
                     HWGCIPVKSLLHSAPTLDSPTPGGDWGTHWKASQKRMREQVSALMQGLTKTLKQAGVA
                     LVEGKGYVASPNSVRVGGALLETDAIILATGSKPATIPAAGLGDESSSLENWVVGNSN
                     DFLSLDDLPKSLLIVGAGAIGCEFADLMKRYGVEVCLAEMLPQILPHFDMQIAQTLSR
                     SFKKRGIEVRTGTGLNGITPDRSGVLATFQDGSSFSTDRVLVAVGRKPRIEDEVIAGV
                     NIEISDGAVVVNEHFQTSVPNLFAVGDMIGGAYLLAHAAEAQGEHAALTALGMTTSGK
                     SRSTMLIPACVYTEPEVATVGLSRDQAKAKGLECVEGKAYYLANGRAVATEQAEGVAK
                     VVAQSKTGQVIGVHICGAFATELIAAATLAMNLNGTVADMAGTVWAHPTCSEVLKQAA
                     LSCLDQLK"
     gene            3231..>3521
                     /locus_tag="PKH07_05395"
     CDS             3231..>3521
                     /locus_tag="PKH07_05395"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNT34418.1"
                     /translation="MKRVLITGAAGFIGSHLAEYFLKHGAEVIGVDNYLTGSPKNIQL
                     LQRHREFRFIEHDVCEPYTIDQPLDGVLHFASPASPPDFQRIPLETLFVSSYG"
BASE COUNT          781 a          998 c          946 g          796 t
ORIGIN      
        1 tcttgcggtg tatagaaccg ctccaccgga gtgtggttgg atgtcggtga aagatactgc
       61 ccaatgacga caagatcggt ttctgcatcc cgaaggtctc ggcaggcttc gaggatttcc
      121 tgaactgtct cgcccaaacc aatcatcaga ctggatttcg ttacaatatg cggagcctct
      181 gctttcaccc tgcggagaag atcaagagac aggtggtaat ctgccccggg gcgcatgaat
      241 ggaaagagcc gcggtaccac ttcgatgttg tgtccccaga catcgggtcg cgcttcaatg
      301 actctggcca ccgattccgg ggaacccagg aaatccggtg tgaggatttc gatacgtggc
      361 tttgacggca atcctttcag agcacctatg acttttgcga attgtgcagc gcctttgtcg
      421 ttgagatcgt cccgtgtcac agacgtcaca acaacgtatc tcagaccgag gcgtgatacc
      481 gcctcagcaa ccttcgacgg ctcatcagga tcgggaagag ccggtgtccc agaggagacg
      541 ttgcagtagc ggcaattccg agtacataca gcgcccagta tcatgaacgt cgcggttcgc
      601 tgcccatagc attcagcgcg gttcggacag gccgcttcct cacagaccgt gcagagaccc
      661 gactcgtcaa gcaccaaaga ggtcttcatg gcgctagggg aagggcccag cggctgcttg
      721 agccagttgg gaattcggcg cttcatcggc ctgcctcctc aaaccgctcc agcatcgcat
      781 cggtcaacgc ctgttggagc agcacccgcg tgaatggaaa gccgaggctc tgaagatcaa
      841 tgcaggcaga ctgcatgagc acccccatcg tcggatcacg aaacacatcg ggaacagaga
      901 acggcaccag cgagaagaag ccctgctgaa gccacacgtg ccgaaatctc cgctgtgcgc
      961 tcccaccgat cttcttttgc tccatcgcgg cagagacgtc cttctgtttg acagtcgcca
     1021 gcagatcgcc ttgggagaaa tgcgcaaggc acaactgtct tgcgtcttgc ggtacatctc
     1081 tgaatctggg aacaacactg gggcgagaga cgggaatgcc gagacggtga aagcctttca
     1141 taaatgcgtc gttcacaagt ccgtagagat catcgcctgt gttgacttca aacggattga
     1201 tgcgcggaat tgcaaatccg taggaaaggt ctttgcaatg atagacaaga gcgcctccgg
     1261 ttggacgctt gcaccaggga atgctctgtg attcggcttt ctcaagatcg agaatttgct
     1321 caggtttctg cccatagccg aaagagaagg cgggtggtga ccagcgatac catcgcagat
     1381 acgccgatat tccatcctca gcacactgat ggacaagcgc ctgatcccga gccatgtttt
     1441 cggttgcagc ggcgggttca cagaaggaaa tcttgagttg catcgttcag tttcacttca
     1501 gctgatcaag gcatgagagc gcagcctgct tgagtacctc gctacaggtt ggatgcgccc
     1561 agacggttcc tgccatatcc gcaacggtac cgttcagatt cattgccagc gttgcagccg
     1621 cgatgagctc cgttgcgaag gcgccgcaga tgtgaacgcc gatcacctgc cccgtcttgg
     1681 attgggcaac gacctttgcc acaccttcag cctgctcggt cgcgaccgct cttccattgg
     1741 cgaggtagta ggctttgcct tcaacgcact ccagaccctt tgccttcgcc tggtcacggc
     1801 tgagtccgac cgttgccact tctggttccg tgtaaacaca tgcaggtatc agcattgtgc
     1861 tcctgctttt gcctgaagtg gtcatgccga gcgccgtgag agcagcatgc tcgccctggg
     1921 cttctgcagc atgcgccaac agataagcgc ctccgatcat gtcacccacc gcaaagaggt
     1981 tgggaacgga cgtttgaaaa tgttcgttga ccacgactgc gccgtcggag atttctatgt
     2041 tcactccggc aataacttca tcttcaatcc ggggcttcct tcccacggcg accagaactc
     2101 tgtcagtgga aaaagaggaa ccatcctgga aagtggcgag cacaccgcta cggtcaggag
     2161 tgatcccatt cagtccagtt cccgttcgca cttcgatgcc gcgcttcttg aaacttcgac
     2221 tgagtgtctg agcgatctgc atgtcgaagt gaggaagtat ctgcgggagc atttcagcca
     2281 ggcacacttc cacgccgtag cgtttcatca ggtcggcaaa ctcacagcct attgctccag
     2341 cgcctacgat gagcagcgac ttgggaaggt catcgagact caggaaatca ttgctgtttc
     2401 cgaccaccca gttctccaga gaacttgact cgtctcccag tcctgctgcc ggaatcgttg
     2461 ctggttttga acctgttgcc aggatgatgg catccgtctc aagcagggct cctccaactc
     2521 ggactgagtt tgggctggcg acatatcctt tgccctcaac cagagcgact ccggcttgtt
     2581 tcagtgtttt ggtgaggccc tgcatcagag ccgatacctg ttccctcata cgtttctgag
     2641 acgctttcca gtgagtgccc caatctcccc cgggtgtcgg agagtcgagt gtgggcgcag
     2701 aatgaagcag cgacttgact ggaatgcatc cccagtggag acacgttccg ccgatggcct
     2761 gcgcctcgac gagtgcgacc ctcgctttgt gacgggctgc ggtcagcgcg gcggtgtagc
     2821 cccccggtcc ccctcccaaa acgattagat tatacctttc tgcggccatg gtttctcaac
     2881 tctcctcgtg acacagagtc caaaagaagc ctgtcatttg ctgaacgggt gtccatgaga
     2941 gtgggcatta cccacctccc tgcaggcgct ggcagccctg caaggatacc tgcccagcac
     3001 tgacggatca cgaaagcaac gttccatgga tggatattaa caggacgcac cagaagtgga
     3061 agaacaatga tgaactgcct tggcaggaaa acagcaatct tcaggctaaa cgattcagca
     3121 ccgcaatcca tcaaaatgcc tgccgttgtt ggtcttctgc gcttgacgca tcggagtcgg
     3181 tgtggctaga ctctgatatg gaatctgttg ctgaaggaga ggccacttca atgaaaagag
     3241 ttttgatcac aggtgccgct ggatttattg gatctcatct tgcggagtac ttcctcaagc
     3301 acggagccga agtcattggc gtggacaact atctgaccgg atcgccaaag aacatccagt
     3361 tgcttcaacg gcacagagag tttcgtttca tcgaacacga tgtctgcgaa ccctacacaa
     3421 ttgatcagcc acttgatggt gttctgcact ttgcgtctcc agccagtccg cccgattttc
     3481 agaggattcc tctggaaacg ctgtttgtct cttcatacgg a
//
LOCUS       DAOQUC010000243         3520 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_30387_length_3520_cov_3.356421, whole genome shotgun sequence.
ACCESSION   DAOQUC010000243 DAOQUC010000000
VERSION     DAOQUC010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3520)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3520)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3520
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30387_length_3520_cov_3.356421"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1506)
                     /locus_tag="PKH07_05400"
     CDS             complement(<1..1506)
                     /locus_tag="PKH07_05400"
                     /inference="COORDINATES: protein motif:HMM:NF018038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HNT34419.1"
                     /translation="MFHKRFRFWAMLLCCFGVASIGFCAEQEAVTPEMIAKITEAAPA
                     SAQVSPMKPRTLLVFSLCNGFVHNAIAVGAKTLEILGQKTGAFSVVVSDDIAMFEPEN
                     IKQFDAICFNNPTGELFLPKDFDALDEAQKQQAQERDARLKKTLLEYIRSGKGIVGIH
                     AATDAFYQWAEFGELMGGYFNGHPWNEDVTVKVDDPLHPVNAAFDGKDFVIADEIYQF
                     KDPYSRDNLRVLLSLNTSLTNMDKGDAIRRNDNDFAVSWVRSYGEGRVFYCSLGHRKE
                     VFWNPQILKHYLDGIQYAFGDLKADSTPSNRATDKNEWRSLFNGKDLTGWQCKPGSWV
                     VVDGVLERRGGGDIWSVGRFGDFVLELEFNVGKGTNSGVFFRTDNIADCVQTGIEMQV
                     LDSYGVENPSKHDCGSIYDCLAPTKNMARPAGEWNKAVIVAKDNKIEIDLNGERIIDM
                     DLNQWTEANKNPDGSGNKFRIAYKDMVRDGHIGFQDHGNPVSYRNIRIRKLK"
     gene            1864..2934
                     /locus_tag="PKH07_05405"
     CDS             1864..2934
                     /locus_tag="PKH07_05405"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNT34420.1"
                     /translation="MRILFLVPWPDQSGPFRFRIGQYLPFLQLNGIDYEIRPFMSPEF
                     FEIVYRKGHTVGKLAHFMESCAGRFLDTLRARKHDLLFIQREVFPYGPALLERILSLW
                     NRPIVFDFDDAIYLPASHSPNKSLAWLKCPSKTATTVRLATRVIVGNSVLEQYARRIN
                     PNVVVLPTPVDTDEIRPRENVPKQDKVTIGWTGSPSTVAYLHGLDSVFQEISRHYPIR
                     VEVYGGLYHCEGVETRSTPEWTRTGELDALNRFDIGVMPLTDDEWTRGKCGFKILEYM
                     AAGIPTVASAVGANNEIVMAGSTGLLASTQSEWIDHLETLLGNPRLRSEMGANGRFRA
                     VQEYSVQVNAPKFLDALQIYRS"
     gene            complement(2922..>3520)
                     /locus_tag="PKH07_05410"
     CDS             complement(2922..>3520)
                     /locus_tag="PKH07_05410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34421.1"
                     /translation="AVDLIPIEEIESFALSNEDFELQRKYEQLDQLVQSRYANEGERQ
                     QRLKRVKELRELTARVESERAKKVSGNIYQDMDMSQYYKGNTFDVSPGLVIEEVVDPT
                     MQMPGDAASTESYWESSVDRTNKDRSRERLQERLDANRRRTRNLAEPTPRRSRSDSRS
                     TTDRTTVRTRTGTSIPSIKSVQTTTIGENPVTIIDYER"
BASE COUNT          823 a          958 c          846 g          893 t
ORIGIN      
        1 cttcagtttt cggatcctga tgttcctgta ggaaactgga ttaccgtggt cttgaaaacc
       61 aatatgccca tctctgacca tgtctttgta ggcgattcgg aacttattgc cagaaccatc
      121 cggattcttg ttggcttccg tccactggtt caaatccatg tcaatgatgc gttctccatt
      181 gagatcaatt tcgatcttgt tgtccttggc gacgatgacc gctttgttcc actcgccggc
      241 gggccttgcc atgttcttcg tcggggcaag gcagtcatag atggagccgc agtcgtgctt
      301 gctgggattc tcaacaccat acgaatccaa gacctgcatc tcaatacccg tctgcacaca
      361 gtcagcaatg ttgtcggtac ggaagaacac accgctgttg gttcccttgc caacgttgaa
      421 ctccagttca agaacaaagt caccaaaacg cccgactgac cagatatccc caccaccgcg
      481 ccgttccaac acaccatcca ccaccaccca actgccaggc ttgcactgcc agccggtgag
      541 atccttgcca ttgaacaggc ttcgccattc gttcttgtcc gtggcgcgat tgctgggagt
      601 cgaatcggct ttgagatcgc cgaaggcata ctggatgccg tcgagatagt gcttaagaat
      661 ttggggattc cagaaaactt ccttgcgatg tcccagcgag caatagaaga cccgaccttc
      721 tccgtatgag cgcacccagc tgactgcgaa gtcattgtca ttgcgacgaa ttgcgtcgcc
      781 tttatccatg ttcgtcagtg acgtattcag actgagcagc acgcggagat tgtcccgcga
      841 gtagggatct ttgaactggt agatttcatc ggcgatgaca aaatccttgc cgtcaaaagc
      901 ggcattgacg ggatgaagcg gatcatccac cttgacggtc acatcctcgt tccagggatg
      961 tccattaaaa tagccaccca tgagctcccc gaattccgcc cattggtaga atgcatctgt
     1021 cgcggcatgg atacccacga tgcctttgcc acttcgaatg tactccagaa gggtcttctt
     1081 caaacgtgcg tcgcgttcct gcgcctgctg cttttgcgcc tcatcgagtg catcaaaatc
     1141 cttgggcagg aaaagttccc cagtcggatt attgaaacag atggcatcga actgtttgat
     1201 attctcaggc tcaaacatgg caatgtcatc actgactaca acagaaaatg cccctgtctt
     1261 ctgcccaaga atctccagtg tcttggctcc aactgcaatc gcattgtgaa caaagccgtt
     1321 gcagagactg aaaaccaaca gcgttcgagg cttcatcgga ctgacttgcg ctgaagccgg
     1381 tgctgcctcg gtaatcttcg cgatcatctc aggtgtgacg gcttcctgtt cagcgcagaa
     1441 gccgatggaa gcgaccccaa aacaacaaag aagcattgcc cagaaacgaa atctcttatg
     1501 aaacattgtg ccctgtcctc cctgaatgtt tgttctcggt tcggtcagat cagtttcagt
     1561 cctccgagag gaatggcacc aaaacaaccg aacctttcag attggaattg ttacagatat
     1621 ttgtggatta tctcaatgaa aatcatcggt acatgtggga gatatcaaaa atcagatgca
     1681 ctttgcattc cggccaaggt tcttgacgcc tacgcaaaag attgggatga tgctctcagt
     1741 aaaacagagt tcgagagggg cgtgtccttt gttgagcaag tacccgagtg aacgggcata
     1801 gcccatccgc tcgcaggtgc taacctgttc agaacctttc atgagtggag gcaagtcaga
     1861 ctcttgcgca ttctctttct tgtgccctgg ccagaccaat ccggaccttt ccgatttcga
     1921 atcggccagt atctgccttt tctccaactc aatggaattg attacgagat tcgccctttc
     1981 atgagccccg agttctttga aattgtctac agaaaaggac acaccgtcgg aaaactcgca
     2041 catttcatgg agtcctgcgc cggtcggttt ctcgacacac tccgcgcccg aaagcacgat
     2101 cttctgttca tccaaaggga agtcttcccc tatggtcctg cactgctgga gcgcattctg
     2161 tctctgtgga accgacccat cgtgttcgac tttgatgatg cgatatacct tccagcgtcc
     2221 cattcgccca acaaatcgct tgcctggctg aaatgccctt ccaaaactgc aaccactgtt
     2281 cggcttgcaa ccagggtcat cgttgggaac tcagttctgg agcaatatgc ccgacgcatc
     2341 aacccaaacg tggtcgtgct cccaacaccg gtggacaccg atgagataag gcccagagag
     2401 aatgtgccaa agcaagataa ggtgaccatc ggatggacag gcagtccctc gacagttgca
     2461 tacctgcatg gtctggattc agtctttcag gagattagtc gtcactatcc cattcgggtg
     2521 gaagtctacg gtgggctcta tcactgcgaa ggagtagaaa cccgctccac acctgaatgg
     2581 actcgaaccg gtgagctgga tgcgctcaac cgctttgaca tcggcgtgat gcctcttact
     2641 gacgacgaat ggacacgagg caaatgtgga ttcaagattc ttgagtatat ggcagctggt
     2701 attccgacag tggcctctgc agtgggtgcg aacaatgaga tcgtaatggc tggatcaacc
     2761 ggtcttcttg cctcaactca gtcggaatgg attgatcatc ttgaaacgct tttgggaaat
     2821 ccgcgcctgc gttcagaaat gggagccaac ggccgatttc gagcggtgca ggaatactcg
     2881 gtgcaagtca acgctcccaa attcctcgat gcactccaga tctatcgttc atagtctata
     2941 atggtgactg gattctcacc gatcgtggtc gtctgaacgg acttgatgga agggatggaa
     3001 gtacctgtcc ttgttcttac tgtcgtccga tcggtagtac tgcggctgtc ggaacgagac
     3061 cgtcgaggag ttggctccgc aagatttctc gtccttcttc ggttggcatc cagccgttct
     3121 tgaagacgtt cacgcgatct gtccttgttg gttctgtcaa cgctgctctc ccaataggat
     3181 tccgtggaag ccgcgtcacc aggcatctgc atcgtggggt ctacgacttc ctcaatgacc
     3241 agccccggtg agacatcaaa agtgtttccc ttgtagtact gcgacatatc catatcctga
     3301 tagatattgc cgctgacttt cttcgcgcgc tcagattcaa ctcgtgcagt caactctcgc
     3361 agttctttaa ccctcttcaa cctttgctgg cgctctccct cattggcata gcgtgactga
     3421 accaactgat ccagctgttc atacttgcgt tgaagttcaa aatcctcatt ggagagtgcg
     3481 aaggattcga tttcctcaat gggaatcaga tccaccgcat
//
LOCUS       DAOQUC010000244         3504 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_30587_length_3504_cov_3.209046, whole genome shotgun sequence.
ACCESSION   DAOQUC010000244 DAOQUC010000000
VERSION     DAOQUC010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3504
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30587_length_3504_cov_3.209046"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            94..2166
                     /locus_tag="PKH07_05415"
     CDS             94..2166
                     /locus_tag="PKH07_05415"
                     /inference="COORDINATES: protein motif:HMM:NF020700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HNT34422.1"
                     /translation="MAYHVSDSQRGVRRGQNVVTLSRVCALQICLSLIFLSGCFSTPL
                     GGEVFRGEWFDYYNRGMHRLRSGDTESAEGDLRRALAQRPGDFDSVRTYGVHFQSYYP
                     HRELGILALERGDWEDALRELESSYQKAPTSRGIHFLNEARRMKIIRSDLDHSDPVVA
                     IELTAGKTLTNQRHFQVKGTVEDDTYVAWISVNDEPVFFELSEPKRSFDEIVSLVPGK
                     NIITVSANDLGGRSSEKSIEVELDIQGPSLSLDETRVVAGAEGRRLWIKGAAFDQNGI
                     AEFRIAEKSLGQSDRIDLAEWFPVESDQDSVDYFLKDLCGNETRGKIHLDGVAQGTDQ
                     GRLPEYLREPVQMGLLHQTPLLLAGLNPTIPSWARIAAVDRGPATFDFGRLSTLNRVY
                     RRDFFINGSVYDADGIAKLNLDGEDYPFISGKEVHFSHFADLQSGTNTLRFVAENGKG
                     VITSSTLVLVLEQPACRQVASRLKLASYGFAAEGNITPATREMTRRLLESKVAEQARF
                     NLLERELLDKILLEHKIAELSEQQWPTLTKLTPADVLLFGKIIEWREGYDIFLKIVNA
                     KTPDVPINEIKIYQPRGVGMDGLYRNIKGLYYLLENEFPLVAGKVVRCDAARIITNLS
                     HAHRIRPGFDLIVYRRGEPIIEDGIDYGCQEDDLGMATVEKVLEQVSYAVTEHECGVK
                     TGDFVITR"
     gene            2256..3248
                     /locus_tag="PKH07_05420"
     CDS             2256..3248
                     /locus_tag="PKH07_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015735632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 130 protein"
                     /protein_id="HNT34423.1"
                     /translation="MAEMDLANLKSSSVVKRYPGNPVLSAKDIPYHATLVFNAGVAKF
                     RDRYVMVFRNDYGVEGKSLQGTNLGIAFSDDGMHWKVSDKPCFTMKTEEIRRAYDPRL
                     TVVEGRCYMCFAVDTQHGIRGGIAVTDDLEHFEILSLSTPDNRNMVLFPEKINGNFAR
                     LERPFPIYGRGAPEAFDIWYSDSPDCKYWGNSNLVLGSEQVPFCNSKIGPAAPPVKTE
                     RGWLTLFHAVDKQPGQVFDSWDGRWGKRYTMGIMLLDLNEPWKIVGMSPEPLMVPELD
                     YPYEMKGFRGSVLFPGGMILEDDGEVKIYYGAADTVEALATARVEDLLALCVSG"
     gene            3310..>3504
                     /locus_tag="PKH07_05425"
     CDS             3310..>3504
                     /locus_tag="PKH07_05425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34424.1"
                     /translation="MNTQDTHSDQVRGSWIESLLAKRQQIDQEIEQAREPVTILFSDI
                     VGYTRYVRTYGDRDGRLLTHK"
BASE COUNT          823 a          852 c          998 g          831 t
ORIGIN      
        1 tctgcgtgag ttcaccgact ccgaagactg cgcgttgatg gtcgtgaacg tgaagaccta
       61 tctcgtggtc tcccagtttg aggatgtgag agaatggcat atcatgtgag cgattcgcaa
      121 cggggagtca gaagggggca gaacgtggta acactgtcaa gagtctgtgc acttcagatc
      181 tgtctgagcc tgattttcct cagtggatgc ttctcgactc cgctgggtgg agaggtcttt
      241 cgcggcgaat ggtttgacta ctacaatcgg ggcatgcacc gtctgaggtc tggtgatacg
      301 gaatctgctg aaggtgatct tcggcgcgca ctcgctcagc gccctggtga tttcgattca
      361 gtgcgcacct atggcgttca tttccagtcc tactatcctc atcgtgagct cggcattttg
      421 gcgctggaac ggggtgactg ggaagacgcg cttcgggaac tggagtcttc ctatcagaaa
      481 gcacccacgt ccagaggcat tcattttctg aatgaagcgc gccggatgaa gatcatccgc
      541 agtgacttgg atcattctga tccggtggta gccatcgagt tgaccgctgg caagacttta
      601 acgaatcaga ggcatttcca ggtcaagggc acggtcgagg atgatacgta cgttgcgtgg
      661 ataagtgtca acgatgaacc ggtcttcttc gaactgagcg aaccaaagag gtccttcgat
      721 gagattgttt cattggtacc tggcaagaac atcattactg tttccgctaa tgacctcgga
      781 ggacgatctt cggagaagtc tatcgaagtg gaacttgaca tacagggacc ttcattgagc
      841 ctcgacgaaa ctcgggtggt tgcaggcgct gaggggcgcc gtctgtggat caagggcgct
      901 gcattcgatc agaacggcat tgccgagttc cgcatcgcag agaaatccct gggacagtca
      961 gatcggattg acctggcgga atggttcccc gtggaatctg accaggattc cgttgactac
     1021 ttcctgaagg atctatgtgg caacgaaacc cggggcaaga ttcatctgga cggtgtggct
     1081 caaggaacgg atcaaggacg gctgcccgaa tatctgcggg aacccgttca aatgggactg
     1141 ctccatcaga cacctttatt gcttgccggt ctcaatccga cgattccttc ttgggctcgc
     1201 attgccgctg tggacagggg gccagcgacc tttgattttg gacggctttc gaccttgaac
     1261 cgagtttacc gaagagactt cttcatcaac ggctcggtct acgacgctga tggcatcgca
     1321 aagttgaatc tcgatggtga ggattacccc ttcatctccg gaaaggaagt ccacttcagt
     1381 cattttgctg acttgcagtc gggaaccaac acgcttcgct ttgtggcgga aaacggcaag
     1441 ggtgtcatca cgtcttccac actggttctc gttctggaac agccagcgtg tcgtcaggtt
     1501 gccagccgac tgaaactggc gtcgtacggc tttgccgcgg aaggcaacat cactcctgca
     1561 actcgggaaa tgacacgtcg tttgctcgaa tcgaaagtgg cagagcaagc acggttcaat
     1621 cttctggaac gtgaactcct cgacaagatt ctgctggagc ataagatcgc cgaactctct
     1681 gaacagcaat ggcctacact gacgaaattg acgcctgctg atgttcttct tttcggaaag
     1741 atcattgagt ggagagaggg ctacgacatc tttctgaaga ttgtcaatgc caaaacgcca
     1801 gatgttccga ttaacgagat caaaatctac cagccacgcg gcgtgggcat ggacgggcta
     1861 tatcgcaata tcaaaggtct ctactatctc ctggagaacg agtttcccct ggttgcaggc
     1921 aaggttgtcc gttgcgacgc agcgcggatc atcacgaacc tcagtcatgc ccatcgcatc
     1981 agaccgggtt tcgatcttat cgtatatcgc cgcggtgagc cgatcatcga agacggcatt
     2041 gactatggtt gccaggaaga tgatttggga atggcaaccg tcgaaaaggt tctggagcag
     2101 gtctcctacg cagtgacgga acatgaatgc ggagtgaaga cgggagactt tgtcatcacg
     2161 aggtaaacag actctgtgtt cagttcgtgg ggtgcggacg ctcgaagatc tgcagccgat
     2221 gaccaacgag ttactacgtc aacaaataag gagcaatggc ggagatggat cttgcgaatc
     2281 tgaaaagcag tagcgttgtc aagaggtatc caggaaatcc cgttctctcg gcgaaggata
     2341 ttccttatca tgccaccttg gttttcaacg ccggtgtggc gaagtttcgg gacagatatg
     2401 tcatggtctt tcgaaacgac tatggtgtgg aaggaaagtc attgcaggga acgaatctgg
     2461 gtattgcctt cagcgatgac ggaatgcact ggaaggtctc cgacaaaccc tgtttcacga
     2521 tgaagaccga ggagattcgc cgtgcctatg atcccagact cactgttgtt gaaggcaggt
     2581 gctacatgtg ttttgctgtt gacacacagc acgggatccg tggaggcatc gctgtcactg
     2641 atgatctcga gcactttgaa attctcagtc tttccactcc ggacaaccga aacatggttc
     2701 tgttcccaga gaagatcaac ggaaacttcg cgcgtctgga gagacccttt cccatctatg
     2761 gacgtggcgc tcctgaggcc tttgatatct ggtattcaga ctctcccgac tgtaagtatt
     2821 ggggcaacag caacctggtt ctggggtctg aacaggtgcc gttctgcaat tccaagattg
     2881 ggccagcagc tccgccagtg aagacagaac gcggttggct gacactgttt catgccgtgg
     2941 acaagcaacc cggccaagtc tttgacagtt gggatggacg ttggggcaaa cgatacacga
     3001 tgggcatcat gctgttggac ttgaatgagc cttggaagat agtcggtatg tcgcctgagc
     3061 ccttgatggt tcccgagttg gactatccgt atgagatgaa gggctttcgc ggcagcgtct
     3121 tgttccccgg tggcatgatc ctggaggacg atggggaagt gaagatttac tacggagcag
     3181 ccgatacggt cgaggctttg gcaacagccc gcgtggaaga tctcctcgca ttgtgcgtat
     3241 cgggatagag gaagaggtac acttccgatt ggcattggag tctgaaacac cttgcggagg
     3301 ggaaatgcga tgaacacaca agacactcat tccgatcaag tcagaggttc ctggatcgag
     3361 tcgctgctcg ccaagcgaca acaaattgat caggagatcg aacaggcgcg cgagccagtg
     3421 actattctct tctccgacat tgtcggctat acgcgatatg tccgaaccta cggagatcgg
     3481 gacggtcgtc tgctaaccca taaa
//
LOCUS       DAOQUC010000245         3501 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_30621_length_3501_cov_3.393790, whole genome shotgun sequence.
ACCESSION   DAOQUC010000245 DAOQUC010000000
VERSION     DAOQUC010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3501
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30621_length_3501_cov_3.393790"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2394)
                     /locus_tag="PKH07_05430"
     CDS             complement(<1..2394)
                     /locus_tag="PKH07_05430"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNT34425.1"
                     /translation="MKILSLLLSLCCVFPFCASAGDWLQFRADATRSGYSEEPLSTDL
                     SAHWVYKPLHPPEPAWKAEDTRMPFDFAPQMVAAKGLVFLGSTVDGSVRAFDADSGKQ
                     RWVFYTDAPVRFAPAIWNDRLLVASDDGYLYALALMSGDLLWKRSGGPHEDMVLGNGR
                     MVSRWPVRGAPVVLDDTVYFAAGIWPSEGIFLYALDPQTGDVRWKNESAGSLELDQPH
                     GGARARSGVSAQGYLAAASDTLVLPTGRAMPAAFERATGEFRYFHLQAQGGGWGARKG
                     AGPFITLLESAGDYLTLSENDLFRTRDGAYLARGIPVQATVVINELLLFCDGKQIKAL
                     NRNSLFEKEERVNRKGDKEEVLVPAKPVWVIDMPSKVGTGMIAAGNTLVVGTQDNRVL
                     TADLATRTVISALEVDGTPLGLASADGRLFVSTDKGTLYCFGKKQPKEAVEVTPGRHP
                     SPYGKNPGFERAAQEIVSGLSGLSGYCLDLGCGEGRLSYELAQRTQFHIIAVDSDPEK
                     VAKARKMLDSAGLYGSRVTVLQRSLEQTTLPNYFADLIVSGNSVKETAIVVPRNEMGR
                     LQRPYGGVYRVGTPGKIESGARGALTGAGSWTHMYADATNVLCSEDTIVQAPLEMLWY
                     TDNDFEMSSRHGRGPGPLFYEGRLFVEGMHGIRAINAYNGHVLWEYPIKNILAAYDQE
                     HLNGAATTGSNFCIEGDTLYLRYENRCLCLDTTTGALKAEFTAPTRPDGTVGKWGYIA
                     CDADRLYGSLCDQGHITVWAFAKAEMEQLYSESNLLFAMDRNTGKVIWTFTPRHSIRH
                     "
     gene            2655..>3501
                     /locus_tag="PKH07_05435"
     CDS             2655..>3501
                     /locus_tag="PKH07_05435"
                     /inference="COORDINATES: protein motif:HMM:NF013662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex protein RnfC"
                     /protein_id="HNT34426.1"
                     /translation="MDNLVDIVREAGVVGAGGAGFPTHVKLSAQAEYLIINGVECEPL
                     LQVDQYLMTEYARPIVETVETVRQQVGAKKAFLTLKEKYTEARQALEAVVKDFPAIEV
                     PVFPSVYPMGDEHVLVYETVGRVVPAGGIPIDVGVVGMNVETIWNIHRAIKGEPLVDT
                     YLSIVGEVPEPMTIKVPLGTVTGDLLSLLGIKVAGLEILEGGPMMGGLTTLDTPLQKT
                     SKGLFLLKPTNPVIYRRKQPVSTFLKIARAACCHCNFCTENCPRYLLGHPLEPHRIMW
                     GVSIGE"
BASE COUNT          786 a         1008 c          931 g          776 t
ORIGIN      
        1 gtgacgaatg gaatgtctgg gtgtaaatgt ccagatcacc tttcccgtgt tcctgtccat
       61 agcgaaaaga agattcgatt cgctgtaaag ctgctccatt tctgcttttg caaaggccca
      121 tacggtgatg tgtccctggt cacataacga accgtagagc cggtctgcgt cacaggcgat
      181 gtaaccccat ttacccactg tgccgtcagg tcgtgttgga gcagtgaact cggctttcag
      241 agcacccgtc gttgtgtcta gacagagaca tcggttctcg taccggaggt agagcgtgtc
      301 tccctcgatg cagaagttgc tgcctgtggt ggcggcaccg ttcaaatgct cctgatcgta
      361 ggcggccaga atgttcttga tcggatactc ccagaggaca tgcccattgt acgcattgat
      421 ggcacgaatc ccgtgcattc cctcgacaaa cagacggcct tcgtaaaaga gcggacctgg
      481 ccccctgcca tgtcgcgatg acatctcaaa gtcgttgtcc gtataccaga gcatttccaa
      541 gggcgcttgg acaatggtat cttcggaaca gagcacgttt gtggcatccg cgtacatgtg
      601 cgtccaggaa cccgcgcctg tcaaagcccc acgggcacca gactcaatct ttcctggtgt
      661 cccaactcga taaacgcccc catagggacg ctgtagtcgc cccatctcat ttcggggaac
      721 gacaatcgca gtttctttga ccgagttgcc cgagacgatc aagtccgcga aatagttcgg
      781 gagcgttgtc tgttcaagac tacgctggag tacggtcaca cgagagccgt agagccctgc
      841 agaatccagc atctttcgcg ccttggcaac cttctccgga tcagaatcca ccgcgataat
      901 atgaaactgt gtgcgttgtg ccagctcata ggagagcctg ccttcgccac agccaagatc
      961 gaggcagtag ccactcagac ccgacaaacc ggacacgatt tcctgagcag cccgctcaaa
     1021 tcctgggttt ttgccgtaag gcgatggatg ccgaccaggt gtgacttcaa ccgcctcctt
     1081 tggttgtttc ttgccgaaac aatagagggt acctttgtct gtgcttacga aaagtctccc
     1141 atccgccgac gccaaaccca gaggcgtgcc gtctacttcc agcgctgata tgacagtccg
     1201 agtcgccaag tcggcagtca gaactctgtt gtcttgagtt cccacaacaa gagtgttccc
     1261 agcggcaatc atccctgttc caaccttgct cggcatatca attacccaga ccggcttggc
     1321 gggtacaagg acttcttctt tatctccctt tcgattgacc cgttcctctt tctcaaagag
     1381 gctgtttctg ttcaacgcct tgatctgttt tccgtcacag aagaggagga gttcgttgat
     1441 gacgactgtg gcttgaacgg gaattccccg cgccagataa gcgccatccc gagttcggaa
     1501 aagatcattt tcactcagtg tcaagtaatc gcccgccgac tccaggagcg tgatgaacgg
     1561 tcccgcgccc tttcgggcac cccagccacc gccctgtgcc tgcagatgga aatatcgaaa
     1621 ctctccagta gcgcgttcaa aagccgcggg catggcgcgt ccggtcggca gcacaagggt
     1681 gtcacttgcc gcagcaagat agccctgggc ggagacgccg ctcctggcgc gagcgccacc
     1741 gtggggttga tcgagttcca gagaaccagc tgactcattc ttccagcgga catctcccgt
     1801 ctggggatcc aaggcataga ggaagattcc ttccgatggc cagattcccg ccgcgaaata
     1861 tactgtatca tccagcacaa ccggagctcc tcgaaccggc cagcgcgaca ccatgcgccc
     1921 attgcccaga accatgtctt catggggtcc tccagagcgc ttccaaagca ggtctccgga
     1981 catcaaagcc aacgcataga gatacccatc atcgctggcg acgagaagcc ggtcgttcca
     2041 aatcgcaggg gcaaatcgaa ccggagcatc cgtgtagaag acccagcgct gtttgcctga
     2101 gtcggcatcg aaggcgcgca ccgaaccatc tacggtactg ccgagaaaga ccaaaccttt
     2161 ggctgccacc atctgaggcg caaaatcgaa aggcattcgg gtgtcttcag ccttccatgc
     2221 aggttctggc ggatgcaaag gcttgtagac ccaatgtgca ctcagatctg tggaaagagg
     2281 ctcctcggaa tacccactgc gggtggcgtc cgctctgaac tgaagccagt ctcccgctga
     2341 cgcacaaaac ggaaacacac agcacaacga caacaacaga gatagtatct tcatcggctt
     2401 acaaacctca caatagttgt catttcaatc tccttggaac tgtattcttg cggtgccctg
     2461 ttggcaagct ggtaatcggg gatcggtggc atcactgacg aggacagtgg ccacaggttg
     2521 ggcaggaacg attccctggc ggcaactacg ccttctgagc gctcacccag ccccctgaat
     2581 ctttcgcttg cgtcattttc gtgtccttcc tatgattcga aagtgctatg agcccattgg
     2641 cgaggtggag ttggatggac aaccttgtgg atatcgttcg agaggctgga gtagtgggcg
     2701 cgggtggtgc cggattcccg actcatgtca aactctctgc gcaggcagaa tacttgatca
     2761 tcaacggagt cgaatgcgag cccttgctcc aggtcgatca atacctgatg accgagtacg
     2821 cccggccgat cgtggagacg gtcgaaacgg ttcgacaaca ggtcggagcc aagaaagcct
     2881 tcttgacact caaggagaaa tacacggaag cgcgtcaggc tttggaagcc gtagtcaagg
     2941 attttccagc catcgaggtt cccgtgttcc ccagcgtcta tcccatggga gacgagcatg
     3001 tactggtcta tgagacggtg ggtagagtgg ttcccgccgg cggcattccc attgatgttg
     3061 gcgtggttgg catgaatgtc gagaccatct ggaacattca tcgagccatc aagggggaac
     3121 cccttgttga cacttatctg agcattgttg gagaagttcc ggaaccgatg acgatcaaag
     3181 tgccacttgg aacagtgacc ggcgatctgc tctccctgtt gggcatcaag gtcgctggcc
     3241 tggaaatcct cgaaggcgga cccatgatgg gcggtttgac cactttggac acgcctcttc
     3301 agaaaacatc caaagggctg tttcttctca aacccaccaa ccctgtgatc tatcgtcgca
     3361 agcagccggt cagcacattc ttgaagattg cccgcgccgc ctgctgtcac tgcaatttct
     3421 gcacggagaa ctgcccgcgc tatctgctgg gtcatcccct ggaaccgcat cggatcatgt
     3481 ggggcgtttc catcggtgag c
//
LOCUS       DAOQUC010000246         3499 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_30642_length_3499_cov_2.863531, whole genome shotgun sequence.
ACCESSION   DAOQUC010000246 DAOQUC010000000
VERSION     DAOQUC010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3499
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30642_length_3499_cov_2.863531"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1888)
                     /locus_tag="PKH07_05440"
     CDS             complement(<1..1888)
                     /locus_tag="PKH07_05440"
                     /inference="COORDINATES: protein motif:HMM:NF019236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HNT34427.1"
                     /translation="MPAAAPQQAQAPVQVEGDQDPSLAATSDAGKWAGIYFMDTSLDG
                     VCKLNNVLVQDAVIGVRMDRCSPIITNSMVEKCSRTGLLAFDNAYPVFDRVIVRDNGY
                     QGIWCDSYSAPSITGCQISNNGSTLWSASGLRVTNHSSPVVAENEIANNGVGIRIEDR
                     SCPNLGNVENTYENDDGRNTIRENAYWDIYNDSGSRIMAQNNFWASTHVPTIDKRIYD
                     DDENPVKGQVVIEPLGRSATATPTRTPTMSVTPTVPTDTPTFTRPPTKTPTSTPTAVY
                     PPSEIVADTTWSGRIYIDHDVTVKANITLFIKPGTVINVDASVVEPRIIVDGGRILAL
                     GQIENTIKFLPATTTQMWGGIEVGPARNLQSEFRFTELHRARKGLKIKNSSPLIQNCL
                     FAQCDIGIELGASVDLWAPATEPKLRCNIFRHNVTGIYMSGEKVKPDLGNSALTGTHL
                     PDPGLNSFIGNSIYDIHVKELTEGSQILAEGNYFYLDNSPSGFLERIEDPSILALRVH
                     APLRFEEGFEDVSNYSLEYVTKKGGCNEAISCDINADGLPDLVLANDGPDQVLLNAGY
                     GRFMDRTLNPLRISSPDVLTRAVLAEDLNNDGLIDLIFGGRDYLAVFIQSMSSTGVRT
                     FANRT"
     gene            complement(1897..>3499)
                     /locus_tag="PKH07_05445"
     CDS             complement(1897..>3499)
                     /locus_tag="PKH07_05445"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HNT34428.1"
                     /translation="PIQLGERMALKGEDGVDVCVLQGAANSPYPILRSSGRSDLLIQN
                     LKFQNMQRTSGNGGAIELVNSGGTIQGCVFYNNAATDGGAVWISPPATQRFTIQNNQF
                     NSNSARYGGGLYVQAGALSVDISNNTFRENTATGGPGGGFYISGSQRGSILRNAFVKN
                     VSTSGTGALGGGFGLSGSFTGNIQENTFVENRAFTGGGFAASQVVGSISKNLFYSNQA
                     TNIDIFNWGQGGGFYLSSCTQITENVFSNNSNGAFSGSATALISRNLLSGNSGNGYGA
                     MNASCQGPISNNYFLYNAISGTDELVGSSLRGNSPSIENNTFVGRGRLGEALVRVNST
                     STTLINNVFVGTDVAINQQTDQSLFIQYNDFYSITNILYVMSTPEGNNLTAIQNKYAN
                     FRNNYNHAPGIVGENQVSGVWTQIPVYSPANNTTTLSDNTKSWVLNQWRGAMLNLGTT
                     TDRRHFPILSNTNTQIIVLGNLTEVDIAYTNPYSIDDYHLASDSLNVDRGTAAVILDD
                     FEEMRDRQIRQIQQTFDSTLVRMSI"
BASE COUNT          779 a          927 c          954 g          839 t
ORIGIN      
        1 tggttctgtt ggcaaaagtg cgaacgccag ttgaactcat ggattggatg aatacggcca
       61 gatagtcgcg accaccaaaa atcagatcga tcaggccgtc gttgtttaga tcttctgcca
      121 gcaccgcccg cgtcaacaca tcgggcgagg aaatgcgaag cggattgagt gtgcgatcca
      181 tgaagcgacc atagcccgca ttcagaagca cctgatcggg tccgtcgttg gcaagcacca
      241 agtcgggcag accatcggcg ttgatgtcgc acgagatcgc ctcattgcag ccacccttct
      301 tggtcacgta ttccagactg tagttggaaa catcctcgaa accttcttcg aaccgcaatg
      361 gcgcgtgaac tcgaagggca agaatggaag gatcttcgat ccgctccagg aatccggacg
      421 gtgagttatc caggtagaag tagttgcctt ccgccaggat ttgactgccc tcggtcagct
      481 ccttgacatg aatgtcatag atgctgttgc cgataaagga gttcagaccc ggatcgggca
      541 gatgggttcc ggtgagtgcg gagttgccca gatcaggctt aaccttttct ccgctcatgt
      601 agatgcccgt aacgttgtgt ctgaagatgt tgcagcgcaa cttcggctct gttgccggcg
      661 cccacaaatc cactgatgcg cccaattcga taccgatgtc gcattgcgca aacaggcagt
      721 tctggatcag cggtgaggaa ttcttgatct tgagaccctt gcgggcacga tgcagctccg
      781 taaatcggaa ttctgactgc aggtttcttg caggcccaac ctcaatacca ccccacatct
      841 gagtcgttgt tgctggcagg aatttgatgg tgttttcgat ctgacccaac gccagaatgc
      901 gtccaccgtc aacaatgatc cgaggctcca caaccgaagc gtccacattg atcaccgttc
      961 ccggtttgat gaacaacgtg atatttgcct tcacggtcac atcatggtca atgtaaatac
     1021 gtcctgacca tgtggtgtcg gcaacgatct ccgacggcgg atacaccgca gttggcgtac
     1081 tggtcggcgt cttggtcggc ggacgtgtga acgtcggcgt gtccgtcggc accgttggcg
     1141 taacgctcat tgtcggggta cgagtcggtg tcgccgtggc actgcgtcca agtggctcga
     1201 tgaccacctg ccctttgacc ggattctcat catcgtcata gatccgcttg tcaatggttg
     1261 gcacatgtgt cgaagcccag aaattgttct gtgccatgat acggctgccg gagtcattgt
     1321 agatatccca gtaggcgttc tcgcggatcg tgttgcgtcc gtcgtcattc tcgtaagtgt
     1381 tctccacatt gccaagattc ggacaggaac gatcctcgat tcggatgccc acaccattgt
     1441 tggcgatctc attctctgcc acaacgggtg aagagtggtt ggtcactcga agccccgatg
     1501 cactccaaag cgtgctgccg ttgttgctga tctggcagcc cgtgatcgaa ggcgcggaat
     1561 acgaatcgca ccagataccc tggtagccat tgtctcgaac gatgactcgg tcgaaaacgg
     1621 gatatgcatt atcgaaggcg aggagccctg tgcgggaaca cttctcaacc attgagttgg
     1681 tgataatcgg agagcagcga tccatgcgga caccgatcac cgcgtcctga accagaacgt
     1741 tgttgagttt gcagacaccg tccagactgg tatccatgaa atagatacct gcccacttgc
     1801 ctgcatcact ggtggcagca agcgagggat cctgatcccc ttccacctgc accggtgcct
     1861 gcgcctgttg cggagcggct gccggcactg gacgaatcag atactcatcc gcaccaatgt
     1921 cgaatcgaag gtttgttgga tttgccggat ctggcggtcg cgcatctcct cgaagtcgtc
     1981 aaggatgact gcagccgttc cgcgatccac gttcagagaa tcggaagcca gatggtagtc
     2041 atcaatgctg tatgggtttg tgtaggcgat atccacctca gtcaggttcc ccagcacaat
     2101 aatctgagtg ttggtgttgc tcagaatcgg gaaatgacgt cggtcggtcg tggtgccaag
     2161 attcagcatc gcaccgcgcc attggttgag aacccaggac ttggtgttgt ccgacaaggt
     2221 cgtggtattg tttgcaggac tgtagaccgg aatctgcgtc cagacaccgg aaacctgatt
     2281 ctctccaacg atccccggag catggttgta gttgtttcgg aagttggcat acttgttctg
     2341 aatcgcggtg agattgttgc cttccggagt gctcatcacg tagaggatgt tggtgatgct
     2401 gtagaaatcg ttatattgga tgaacaagga ctgatccgtc tgttggttta tggcaacatc
     2461 cgtccctaca aagacattgt taatgagcgt cgtactggtt gaattgacgc gaacgagcgc
     2521 ttcgccaagg cgtcctcgac ccacaaaagt gttgttctca atggaaggtg agttgccgcg
     2581 cagtgacgaa ccaaccaact catcggtgcc agagatcgca ttgtagagga aatagttatt
     2641 ggagatcgga ccttggcacg atgcattcat cgcaccatag ccgttgccac tgtttccgct
     2701 gagcaagttc ctgctgatga gcgccgtcgc tgagccactg aaggctccat tggaattgtt
     2761 ggagaaaacg ttctcggtaa tctgtgtgca ggagctcaaa tagaagccac caccttgtcc
     2821 ccagttaaat atgtctatat tggtggcctg attgctgtag aagaggttct tggatatgct
     2881 tccaacaact tgagatgcag caaatccacc ccctgtgaag gctctgtttt ctacgaaggt
     2941 gttctcttgg atgttccccg taaacgagcc actcagccca aagccacccc ccaaagcgcc
     3001 ggtgccgctc gttgacacgt tcttgacaaa ggcgttccga aggattgatc ctctctgact
     3061 accagaaatg tagaagccac cgccaggtcc acctgttgcc gtattctcac ggaaagtgtt
     3121 gttggagatg tccacactca acgctccagc ctgaacgtag agcccaccac cgtagcgagc
     3181 ggagttggag ttgaactgat tgttctgaat ggtgaatcgc tgagtggccg gcggcgaaat
     3241 ccagactgcg ccgccatcgg ttgccgcgtt gttatagaac acacagccct ggatcgtgcc
     3301 accgctgttc accaactcga tggcaccacc gtttcctgaa gtgcgttgca tgttctggaa
     3361 cttcaggttt tgaatcagca ggtcacttcg gccgctggaa cgaagaattg gataggggga
     3421 gtttgcagcg ccttggagga cgcacacatc aacaccatct tcacccttca gtgccatgcg
     3481 ctcaccgagt tggatcggg
//
LOCUS       DAOQUC010000247         3497 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_30664_length_3497_cov_2.690006, whole genome shotgun sequence.
ACCESSION   DAOQUC010000247 DAOQUC010000000
VERSION     DAOQUC010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3497
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30664_length_3497_cov_2.690006"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..751)
                     /locus_tag="PKH07_05450"
     CDS             complement(<1..751)
                     /locus_tag="PKH07_05450"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HNT34429.1"
                     /translation="MKIVTRYILREVINPFAIGVFAFTFLFLANGIFRLMDWIVIGGI
                     QGSLVGGMVLALIPFILNVTFPMALLLGCVFAFGRLSEHNEIQALLTCGHTYGRIVRP
                     VILLGLGCTGFLLFWSEYIAPKASLIQNMTTFRIVEHMSPIAVLEEGKFSTRLPGRVL
                     YLQKIDLADHTVHGVAIFEMNGNKIRAALLSPRGRVQYNSQEMTLSLDLENWEVHVDV
                     ESDDPKMATINGRIQMVVDCGSMMGKLLRDKQ"
     gene            complement(748..1260)
                     /locus_tag="PKH07_05455"
     CDS             complement(748..1260)
                     /locus_tag="PKH07_05455"
                     /inference="COORDINATES: protein motif:HMM:NF019099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HNT34430.1"
                     /translation="MTLARHHWLGSDRAMRVSVWVAFGLLLHLLEGALMPRAFPFRLG
                     LAHLAALLALSLDGFGTAMQVSIWRAVVGSLFYGTFLQMSFFLSLTGAVAGTATMALA
                     LLMGKKLFSEIGISLAGAAGSAGAQCLVASLLLGSPMLFLLPVILILGLFGGLLNGVL
                     AHGILEGRTL"
     gene            complement(1288..2223)
                     /gene="truB"
                     /locus_tag="PKH07_05460"
     CDS             complement(1288..2223)
                     /gene="truB"
                     /locus_tag="PKH07_05460"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HNT34431.1"
                     /translation="MTLFGVLNLNKPAGFTSHDAVDAVRKITRIRRIGHTGTLDPMAT
                     GVLPLCVGNATKIAQFLIAQDKEYEVEMTLGLITDSQDTTGKLVEERDWRIVSDEDIR
                     RLPEKFTGTQKQVPPMVSAKHHEGKRLYQLAREGIEVEREPIEIQITQLEILSMELPR
                     IRFRVLSSKGTYIRTLCHDMGLYLGTGGTMSALVRTRCGAFHIKDSALLDDLKSPEDV
                     LGHLHTVDNALSSIPAVEIAETARRRIFQGQPVLGSHVVGVIGKFEAGGLVRIKTSKD
                     ELLAMGKAEISSVHIERLGGGLNVVKPIRLLAEGR"
     gene            complement(2220..2600)
                     /gene="rbfA"
                     /locus_tag="PKH07_05465"
     CDS             complement(2220..2600)
                     /gene="rbfA"
                     /locus_tag="PKH07_05465"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HNT34432.1"
                     /translation="MTSSSYRMERVNELVREEVGRILLLESQDPRFVSVTVTGAKVTS
                     DLNFAKVYVSILGTSEERDSVLSALGKASGFVRRQLGQQVRMKRTPQLTFVYDESLER
                     ANQIYKALEGLDLGSEKPETEETL"
     gene            complement(2694..2975)
                     /locus_tag="PKH07_05470"
     CDS             complement(2694..2975)
                     /locus_tag="PKH07_05470"
                     /inference="COORDINATES: protein motif:HMM:NF016351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HNT34433.1"
                     /translation="MHIGLLQVHVYIPTSESLKDKRQVLRSIKQRLRNSFNVSVAETG
                     QQDLWQRSDLALVMVATDKAHIQETFSNAVRLILNKENVEILEESVEFL"
     gene            3200..>3497
                     /locus_tag="PKH07_05475"
     CDS             3200..>3497
                     /locus_tag="PKH07_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase N-terminal SAM-like
                     domain-containing protein"
                     /protein_id="HNT34434.1"
                     /translation="MVILSKEQEIVELTPTQIIPENTQPCRGTVRVNGDRPKLLDLLR
                     QALRTRHYSRSTEQTYCHWVKRYVHFHNLRHPAEMAEPEINAFMTQLAVKEKVSA"
BASE COUNT          883 a          999 c          848 g          767 t
ORIGIN      
        1 gctgcttgtc acgcagcaat ttccccatca tgcttccaca gtccacaacc atctgtattc
       61 gtccgttgat ggttgccatc ttggggtcat ccgattccac gtccacatgg acttcccagt
      121 tctccaaatc cagactcaga gtcatctcct gggagttgta ctggacgcgt ccccgcggag
      181 acaacagcgc ggcacgtatc ttgttcccat tcatctcgaa gatcgccact ccatgaacgg
      241 tgtgatccgc caagtcaatt ttctgcaggt agagcactct tccagggagt cgcgtgctga
      301 atttgccttc ctccaaaacg gcaattgggg acatgtgctc cacaatccga aaggtggtca
      361 tgttctgaat caaagaagcc ttgggtgcga tgtactccga ccagaagaga agaaagcccg
      421 tgcaccccag acccagaaga attaccggtc tcacgattct cccataggtg tgaccacaag
      481 tcagtagcgc ctgaatctca ttgtgttctg acaaccgccc aaacgcaaaa acgcatccca
      541 acaaaagtgc catgggaaat gtgacgttga ggatgaatgg gatcagcgcc agtaccatac
      601 cgccgaccag actgccctga attcccccaa tcacgatcca gtccatcagt cggaatatcc
      661 cattggcgag aaagagaaat gtgaacgcga acactccaat ggcgaagggg ttgatcacct
      721 ctctgagaat gtaccgagtg acaatcttca cagagttctc ccttcaagga tgccatgtgc
      781 cagcactccg ttcaatagac ccccgaacag tcccagaatc aggattacgg gaagaaggaa
      841 cagcatcgga ctgcccaaaa gcagtgacgc gacgagacat tgtgcaccgg cactgccagc
      901 cgctccggcg aggctgatgc cgatctccga aaacagcttt ttccccatca acagtgccag
      961 agccatcgtg gcagtacccg ctacagcgcc agtcagactc aagaagaaac tcatttgcag
     1021 aaatgttcca tagaagagcg atccaaccac agcccgccag atcgaaacct gcattgccgt
     1081 gccaaaccca tccagactca aggccaacag ggcagccaga tgtgccaacc ccaaacgaaa
     1141 gggaaaggca cgtggcataa gcgcaccctc aagcagatgg agtaatagcc cgaacgccac
     1201 ccagactgag acccgcatcg cacggtctga gcccagccag tgatgtctgg caagcgtcat
     1261 caggggtctt cctgaactca ccacgcttca gcgcccctcc gccaggagtc gtatgggctt
     1321 gaccacattc aagccgcctc ccagtctctc aatgtgaacc gaggagattt cggcttttcc
     1381 catcgccaga agttcatctt tggaagtctt gatgcgaacc agaccacccg cctcgaattt
     1441 accgatcacg ccgaccacat gcgaacccaa aacgggttga ccctgaaaga tacgccttcg
     1501 ggctgtttcg gcgatctcga ccgctggaat cgaggacaag gcattgtcca cagtatgaag
     1561 atgtccgagt acatcttcgg gggacttcaa gtcgtccagc aacgcggagt ccttgatatg
     1621 aaaggcaccg catcgggtgc gtaccaaggc gctcatcgtt ccaccggttc cgagatacaa
     1681 acccatgtca tgacacagtg ttcgaatata agtgcctttg gaactgagga ctcggaatcg
     1741 gattctcggc agctccatgg acaggatttc caactgcgtg atctgaatct caatcggttc
     1801 acgctcaact tcgatccctt cacgggcgag ttgatagagt ctcttgcctt catgatgttt
     1861 ggcggaaacc attggcggaa cctgtttttg ggttccagtg aacttttcag gaagacgccg
     1921 aatatcctca tcggacacaa ttcgccagtc acgctcctca acgagtttgc ccgttgtgtc
     1981 ctgggaatcg gtgatcagac ctagagtcat ctccacttca tactctttgt cctgagcgat
     2041 gagaaactgg gcgatcttgg tggcgttccc gacacacagg ggcaacactc cggtggccat
     2101 cggatccagg gttcccgtgt ggccgatgcg ccggattcgg gtgattttcc tcacggcatc
     2161 caccgcatca tgcgaagtga agccggcagg tttgttcaga ttcagaactc caaaaagtgt
     2221 cataaagttt cttccgtttc aggtttctct gagccgagat ccagtccctc gagtgccttg
     2281 tagatttggt tggcgcgttc cagagattcg tcatagacaa acgtcaactg tggcgttctc
     2341 ttcatccgga cttgctgtcc caactgacgc cggacgaatc cagacgcttt cccgagagcc
     2401 gacagaacgc tgtcccgctc ctcagacgtg ccaagaatac tgacatagac cttcgcgaaa
     2461 ttcaaatccg atgtcacttt ggctcccgtg acggttacgg aaacgaaccg ggggtcttgc
     2521 gattccagaa gcagtattcg accgacctct tcgcgaacca attcgttgac tctttccatt
     2581 cgataagatg aggatgtcat gattcagcac cttgctttcc tacgaaacga gaccccaaag
     2641 gacggtaaac acggattcgg ttatatccaa aatgcctggg gtactcttca cctctagaga
     2701 aactccaccg actcttccaa tatctctacg ttttccttgt tcaatatcag tcttacggcg
     2761 ttggaaaaag tctcctggat atgggctttg tccgttgcca ccatgacaag cgccaagtcc
     2821 gaacgctgcc acagatcttg ctgtcctgtc tccgccacgg acacgttgaa agaattccgc
     2881 agccgttgct tgatgctcct cagcacctgc ctcttgtctt tcaacgattc ggacgtcgga
     2941 atgtatacat gcacctgcaa aagtccaatg tgcatagtga gatggattat aggtaaccat
     3001 gagaaaatcg caagagaact tgcacaaaag atctttcccc gagacagagg aacgacggca
     3061 cagacaactg cgctctgccg caaagggaag cctgagcgaa caagagcagt acgcttgacc
     3121 aagattttac ttgagttgct ctccagaaag ggagtatatt ccaagatgtg gttgtgggtc
     3181 ttctgcttag aagagcgaaa tggtgatttt gagcaaagag caagaaatcg tggaactgac
     3241 gccaacacag atcatcccgg aaaacaccca gccgtgccgt gggactgtgc gcgtgaatgg
     3301 cgacagaccc aaactgcttg atcttttgcg ccaagcgctg cgcactcgcc actacagccg
     3361 cagcaccgag cagacctatt gccattgggt caagcggtat gtccattttc ataatttgcg
     3421 ccaccctgcc gaaatggccg aaccggaaat caacgccttt atgacgcagc tggcggtcaa
     3481 ggagaaagtc agcgcct
//
LOCUS       DAOQUC010000248         3477 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_30899_length_3477_cov_3.409702, whole genome shotgun sequence.
ACCESSION   DAOQUC010000248 DAOQUC010000000
VERSION     DAOQUC010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3477)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3477)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3477
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30899_length_3477_cov_3.409702"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..236)
                     /locus_tag="PKH07_05480"
     CDS             complement(<1..236)
                     /locus_tag="PKH07_05480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34435.1"
                     /translation="MNWRSPLLRLAFEAKALRWGAETNVNRLLAKAADLGKAEKHQIK
                     SRQHQLLKELILHCDAHVPYYRKILRECELIENN"
     gene            complement(233..1426)
                     /locus_tag="PKH07_05485"
     CDS             complement(233..1426)
                     /locus_tag="PKH07_05485"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNT34436.1"
                     /translation="MGEPVNVLMLNYEYPPLGGGAGNATAYLLRELTNLDDVSVTLVT
                     SSTTSERVERISDRIWIHFLDIGKRGNPHYQSNRDLLTYSWQSLRHCRGLIRSNAYNL
                     CHAFFGIPSGVITRMLGLPYIVSLRGSDVPFYNERFRIPDLLLFRRLSVRVWRDAARV
                     VANSQQLRSLALQSSPQQKIEVIPNGVDVAEFQPAPCPLDPTEMRILCVSRLIARKRI
                     DVLIKSLAIVRDLPVSLTLVGTGNEESQLRQMVKELNLGQIVRFEGYVSHDCIAKVYQ
                     QSNLFVLPSANEGMSNTVLEAMACGLPILMRQTGGTEELIQDGSNGFVLHSGSSDEIA
                     GRIRLYCERPEMLSIHGKASRKIAESLSWRNVAQDYHQLYLTIAERRLSLRNSRVLES
                     GRQGR"
     gene            1619..2527
                     /locus_tag="PKH07_05490"
     CDS             1619..2527
                     /locus_tag="PKH07_05490"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HNT34437.1"
                     /translation="MRERLLGKRVLAIVPHQDDEVIGMGGSIAWACEVAESLDLVLVT
                     DGASTLSALTVSGLSSCCRFVGIDPEREADLLAHDASLTEKENGELHHHPDLADRFKM
                     EPDPERLGGRGSVSWGRQRDREFLSVAEQLGVSSSRIHLAYHDPSSPCAIKDGQIALG
                     GRELSPEDRVARYAEVASHYFKRLSPDVVFTMAPYEFAPPPNDHWAMAHGAEQAARDC
                     SIETVFYFHSGIHYQHVLKGGAHLGKRILLPENLWSVKKNGLREYLRWSPDEGWFATA
                     PHSVYKTFCAIFSGEGRYEYVSDNPL"
     gene            complement(2644..3216)
                     /locus_tag="PKH07_05495"
     CDS             complement(2644..3216)
                     /locus_tag="PKH07_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005813203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNT34438.1"
                     /translation="MNSLGRVYVALLHYPVYDRRRRSVSTSLTSMDVHDIARSCKTYG
                     VHAYYIVQPLRTQNLLARKIAEYWQRGPGGGFNANRKEALELIRVTESVQEMQDQILD
                     ETGLEPIYVATDAKRYPWSQSFSTVRNKIRDSDEPFTLLFGTGDGLDESVITESDILL
                     EPIEGPTDYNHLSVRGAAAIILDRLLRVDP"
     gene            complement(3304..3477)
                     /locus_tag="PKH07_05500"
     CDS             complement(3304..3477)
                     /locus_tag="PKH07_05500"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNT34439.1"
                     /translation="MGKIADFYEEQVDTAVNGLTSMLEPVMIVFLGIVVGGVVIAMFM
                     PLFKMTTILKGTG"
BASE COUNT          808 a          909 c          928 g          832 t
ORIGIN      
        1 tcgttgttct caatcaactc gcactctctg agaatctttc gatagtaggg aacatgggca
       61 tcgcagtgca gaatcagctc cttcagcagt tggtgctgac gtgatttgat ctggtgcttt
      121 tcggctttgc ccaagtccgc tgctttggcc aagagtcggt tcacatttgt ctcggcaccc
      181 caccgaagag ccttggcctc aaatgccaat cgaagcaagg gacttcgcca gttcatcttc
      241 cttgcctccc agattcgaga acgcgtgaat tcctgagaga cagccgcctc tcagcaatgg
      301 tgaggtataa ctgatgatag tcttgcgcca cattccgcca gctgagggac tctgcgatct
      361 tcctggacgc ctttccatga attgagagca tttcgggtct ttcgcagtac aggcgaatcc
      421 gtcccgcgat ctcgtctgat gaacccgaat gaagcacgaa gccgttgctt ccgtcctgaa
      481 tcaactcttc ggtgcctcca gtctgtcgca tgagaattgg cagcccacaa gccatcgcct
      541 caagcacagt gttgctcatg ccttcgtttg cagagggcag aacaaacaga ttgctctgct
      601 gatagacttt cgcgatacaa tcatgagaga catacccttc aaatcggaca atttgtccca
      661 ggttcagttc tttcaccatt tggcgaagtt gggattcctc attgcctgtc cccacaagtg
      721 tcagggaaac tggaagatct cttacgatgg ccagagattt gatcagcaca tcaatccgct
      781 ttcgggcaat gagtctcgag acacagagaa tccgcatctc agttggatcc aaagggcacg
      841 gtgccggctg aaattcagcg acatccacgc cgttgggaat gacctcgatc ttctgctgag
      901 gcgatgactg tagtgccagt gagcgaagtt gctgggagtt ggcgactacc cttgcagcat
      961 cgcgccagac tcggacgcta agtcttctga acaacaaaag gtcagggatc cgaaatcgct
     1021 cattgtagaa agggacatcg gagccacgga gcgaaacaat atagggaagc ccaagcatcc
     1081 tggtgatgac tccagacggg atgccaaaga aggcatggca gagattgtag gcgttcgatc
     1141 ggatgagacc acgacagtgt cgaagggact gccatgaata ggtgagcaag tcgcggttgc
     1201 tctgatagtg gggattccct cgcttcccga tgtccagaaa gtgaatccag atacgatccg
     1261 agatccgttc gactcgctct gatgtagttg atgaggtgac aagtgtgact gaaacgtcgt
     1321 caaggtttgt cagttcacgg agcaagtatg ccgttgcatt gccggcaccg ccaccgagag
     1381 gcgggtactc gtaattcagc atgagaacgt tgacaggttc acccattttc attccaggct
     1441 tgttgctgcc tcaatctgta acacgcaccc ttccgagttg gcaagccaga gtgagaattc
     1501 gcagtgtcct gtcaatctct gttgtctgag acgaagaggt cgaatggtgg attgtctgag
     1561 caacgtatgc catatttatc ctgatatctg tctgtctata gaagaaaggg cttaactgat
     1621 gcgagaaagg cttcttggaa aacgtgttct tgccatcgtc cctcaccagg atgacgaagt
     1681 catcggaatg ggtgggagta ttgcctgggc gtgtgaggtt gccgaatctc ttgatctggt
     1741 tctggtgacg gatggcgcgt caacgctatc cgccctgaca gtcagcggac tttcctcgtg
     1801 ttgtcggttt gtcggaatag atccggaacg agaagccgat ctgttggcgc atgatgcctc
     1861 actgacagaa aaagagaatg gagaactgca ccatcatcca gatctcgcag accgtttcaa
     1921 gatggagccg gatcccgaac gccttggcgg aagaggctcc gtctcttggg gaagacagcg
     1981 tgatcgcgaa ttcctctccg ttgcagagca gttgggtgtg tcttcttcac ggattcatct
     2041 ggcatatcat gatcccagtt caccgtgtgc gatcaaggat gggcagatcg ctttgggtgg
     2101 cagggagttg tcccctgagg atcgtgtggc gcggtacgct gaagtggctt cgcactactt
     2161 caagcgtctt tcccctgatg tggtttttac gatggctcca tacgagtttg cccctccgcc
     2221 caacgatcac tgggcaatgg cacatggagc cgaacaggct gcccgagatt gttcgattga
     2281 gacggtgttc tatttccaca gtggaataca ctaccagcac gtacttaaag ggggagcgca
     2341 tctggggaag cgcatcctgt tgcccgagaa tctctggtcg gtcaagaaga acggtctgag
     2401 agaatatctg cgctggagcc ctgatgaagg ctggtttgcc accgcgcctc attcagtcta
     2461 caagaccttc tgcgcaatct tctcaggaga aggacgatat gaatatgtca gcgacaatcc
     2521 tctctgacaa aggaagagag cctcgcccga catacacatc acagctgcct ttcgtgtgcc
     2581 tctgcggaag gtggatgcct cactttgcca caaggaacca agaacgtctg actctccctc
     2641 catctatggg tcaacgcgca agagtctgtc cagaatgatg gctgccgcac cgcgaacgga
     2701 aagatggttg tagtcggttg ggccttcgat aggctccaga aggatgtcgg actccgtgat
     2761 gaccgattca tccaatccgt ctcctgttcc aaacagcaga gtaaacggtt catctgaatc
     2821 tcggatcttg ttgcgaaccg tgctgaaact ctgtgaccag gggtatctct ttgcatcagt
     2881 tgccacgtag atcggctcca ggcccgtctc gtcaagaatc tgatcctgca tttcctgaac
     2941 ggactcggtg acccgaatca gttccagagc ttcctttctg ttggcgttaa aacctccgcc
     3001 cgggcctctc tgccaatact cggcgatttt ccgggcaaga agattctggg ttcggagggg
     3061 ttggacgatg tagtaggcat gcacaccgta tgtcttgcaa ctgcgtgcga tgtcatgcac
     3121 atccatactc gtcaaagacg ttgaaaccga ccgtcttcgt cgatcataga ctggatagtg
     3181 aagcaatgcg acgtaaacgc gtcccaatga attcaacctt ctgcacaatt ccctggatca
     3241 acacgccccc cgttttggca cggagggcgt gaatcgaacc aacctgagat agtgtctgtc
     3301 ggtctagccg gtacccttga gaatcgtggt catcttgaac aagggcatga acatggcgat
     3361 gacgacgcca ccaaccacaa ttcccagaaa cacgatcatc acgggttcca acatcgaggt
     3421 cagaccgttg acggctgtgt ccacctgctc ctcgtagaag tcagcgatct tgcccaa
//
LOCUS       DAOQUC010000249         3475 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_30926_length_3475_cov_3.652047, whole genome shotgun sequence.
ACCESSION   DAOQUC010000249 DAOQUC010000000
VERSION     DAOQUC010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3475)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3475)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3475
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30926_length_3475_cov_3.652047"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(80..622)
                     /locus_tag="PKH07_05505"
     CDS             complement(80..622)
                     /locus_tag="PKH07_05505"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008441721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006596 - polyamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase, pyruvoyl-dependent"
                     /protein_id="HNT34440.1"
                     /translation="MVPKKVFLTKGLGHHKEELRSFELALRDAGIEKCNLVQVSSILP
                     PGCKIIPRKDGLAQLKPGQITFCVLARWQSNEPRRLIVTSIGVAIPNDKSLYGYLSEH
                     HAYGQTEKTAGDYAEDLAAAMLASTLGLEFDENKDWDEQRDVFKLGNKRIVHTRNITQ
                     SAIVRQGGYTTAIAAAVFIL"
     gene            complement(656..1564)
                     /gene="speB"
                     /locus_tag="PKH07_05510"
     CDS             complement(656..1564)
                     /gene="speB"
                     /locus_tag="PKH07_05510"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HNT34441.1"
                     /translation="MIKPPIFESDVNFLGLPLEFSSWDQSKCVVLSAPLDMTTSFRKG
                     TAEGPKAIIAASTQVELYDAEFGDEPALDIGVHTLPSLEPQCVSMQAAVDRVREGVGF
                     IVEQGKIPMLLGGEHSVTIGAAQAFKDKMDKTCFVVIDAHADLRDSYHGTPLSHACAS
                     RRLWEMAPMIQVGIRSYSAEEAQWMREKPSGYTAFPARTVTTRMQAFEESLRILPDQV
                     YISIDLDGLDPSIMPAVGTPEPGGLLWEETLDVLRMVCRMKKVVGFDIVELCPMPGWV
                     APDFLAAKLCYKLLSYACSRVPSSGN"
     gene            complement(1650..1964)
                     /locus_tag="PKH07_05515"
     CDS             complement(1650..1964)
                     /locus_tag="PKH07_05515"
                     /inference="COORDINATES: protein motif:HMM:NF027581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4911 domain-containing protein"
                     /protein_id="HNT34442.1"
                     /translation="MTATRQEKFWWKHALPQERDTISVRAKVDPVIIGLLNGLVDAHD
                     GIACLRTTDPKAGKLEFWVSPNCLDDFQIMLKGLREVADVEIENEENLPYQSAVPNED
                     LR"
     gene            2229..2411
                     /locus_tag="PKH07_05520"
     CDS             2229..2411
                     /locus_tag="PKH07_05520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34443.1"
                     /translation="MTVKEKVLQAVESLPDDASIEGAMERLFFLAKVEKGIQQANTGQ
                     TLSHCQVKESLSKWLK"
     gene            2399..2533
                     /locus_tag="PKH07_05525"
     CDS             2399..2533
                     /locus_tag="PKH07_05525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34444.1"
                     /translation="MAEVRWTLQGADDLDAIAFSTCASLAASKMASEKPERLCGTVTA
                     "
     gene            complement(2677..3312)
                     /locus_tag="PKH07_05530"
     CDS             complement(2677..3312)
                     /locus_tag="PKH07_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNT34445.1"
                     /translation="MRTRTGGFTLIELLIVVAIIGILAAIAIPNFLSAQVRAKVARVT
                     ADLATLAGALETYCVDNNRFPLDSPRDPHYPGDNCERIQDCLTTPIGYLASASGMYDP
                     FRDRNIPPLSGWDRDTVSRYEYWEFTGSYIACNHHPDWGMAYMNGTDRFVGFGEWMLA
                     SYGPDTSYGPPETTDSILYTCHVLYDPTNGATSAGEIVRCRKWGVMESNWH"
     gene            complement(3278..>3475)
                     /locus_tag="PKH07_05535"
     CDS             complement(3278..>3475)
                     /locus_tag="PKH07_05535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34446.1"
                     /translation="WPYRPPARADSLEREVSEERPVVISKPKPYVEILRPYSWMLSTT
                     LVLRSHHGGLDENTHWRIHSD"
BASE COUNT          790 a          981 c          910 g          794 t
ORIGIN      
        1 ctatggagcc ccggcttggc cgctcaggcc acgggctgtc ttggcagcgc cgtacttgtt
       61 tgtcagagcg cctcgacgat cagagaataa agaccgcagc ggcgatggcg gtcgtgtagc
      121 ctccctgacg gacaatggcg gactgtgtga tattccgagt atgaacgatg cgcttgttgc
      181 ccaatttgaa gacatcgcgc tgttcgtccc agtctttgtt ctcatcgaac tccaaaccga
      241 gggtcgaggc cagcattgct gccgcgaggt cttccgcgta gtcgccagcc gtcttttcgg
      301 tctgtccata ggcatgatgc tcagacagat agccgtagag actcttatca ttcgggatgg
      361 caacgcctat ggatgtgacg atcagtcgtc ttggttcgtt gctctgccaa cgggcgagaa
      421 cgcagaacgt aatctgaccc ggtttcaact gagccagtcc atctttgcgg ggaataatct
      481 tgcaacccgg aggcagaatg ctggagacct gcaccaggtt gcatttttcg atccccgcgt
      541 ctcggagcgc cagctcgaaa gatcgcagtt cctctttgtg gtgacccaga cccttggtta
      601 agaacacctt ctttgggacc atctagttct cctctctttg cggatcgttg ctttctcagt
      661 ttcctgagga aggtacccga ctgcaagcat agctcagcag tttgtagcac agtttcgcgg
      721 ccaggaagtc cggagccacc cagcccggca tcgggcacag ctccacaatg tcgaaaccca
      781 ccaccttttt cattcggcag accatcctga gaacatccaa ggtctcctcc cagagaagac
      841 cccccggttc cggagttccg actgccggca tgatcgaagg atcaaggcca tccaagtcta
      901 tcgaaatata cacctgatcg ggcaagattc taaggctttc ctcgaaagcc tgcatccgag
      961 tggtcaccgt tcgggcagga aacgcagtgt atccggaagg cttctcccgc atccactgtg
     1021 cctcctcagc cgaataactc ctgatgccca cctgaatcat gggagccatc tcccacaacc
     1081 gacgactggc gcaagcgtgg ctcagaggtg tgccgtggta tgaatccctc aggtcagcgt
     1141 gggcatcaat gaccacgaag cacgtcttgt ccatcttatc cttgaacgcc tgggccgccc
     1201 caattgttac cgaatgctcc ccgcccagaa gcatgggaat cttgccctgt tcgacaatga
     1261 acccaacgcc ttcgcgaact cggtcaaccg ccgcctgcat cgaaacgcac tgaggttcca
     1321 atgacggcaa agtgtgaacg ccgatatcca gagccggttc atctccaaac tcggcgtcgt
     1381 acaactccac ttgagttgag gcggcaataa tggctttggg tccttctgcg gttccttttc
     1441 gaaagctggt ggtcatgtcc aagggagcgg acaacacaac acatttggat tgatcccaac
     1501 tggagaattc taacggtaag ccgaggaaat tgacatcaga ttcgaagatg ggtggcttga
     1561 tcatccctta cccttcggcc tccaagagtc ccgccaccaa aacgggtaat gcaattgtgt
     1621 catcacaata gactgttgcg gttccagcgt caacggagat cttcattcgg gactgcactt
     1681 tggtaaggca gattctcttc attttcgatt tcgacatcag caacctctcg cagccccttg
     1741 agcatgatct ggaaatcatc cagacagttc ggggaaaccc aaaactccag tttgcccgcc
     1801 ttgggatccg tggttctcaa acaggcaatt ccgtcatggg catccaccag accgttcaac
     1861 aggccgatga tcaccggatc caccttcgcg cgaacggaga tcgtatcacg ctcttggggc
     1921 aaggcatgct tccaccagaa cttctcctgc cttgttgccg tcatgggatc tctctccaag
     1981 cgcgaaagca ccgtatacat atacagcctg agggcagtct gtcaatgaaa aatgagcgga
     2041 ttgctctcgc ctgtccgttg cactcctctt cgattctctg agtcggtagc aacgctgccg
     2101 acaaaacatc tcgcttgctc cggcggcagc gcagagcctc tggcatcgaa gcataacaga
     2161 agtgtgcata gtatatccta cgtggcctgc tatctgattc attactcgca gcgtggtaga
     2221 ttgcaggtat gacagttaag gaaaaggtct tacaggcagt tgaatccctt ccggatgatg
     2281 catccatcga gggtgccatg gagcgtttgt tctttcttgc caaggttgag aagggaatcc
     2341 aacaagccaa taccggacaa accctctcgc attgccaagt gaaggaaagt ctctccaaat
     2401 ggctgaagtg agatggacgt tgcagggagc cgacgatctc gacgcaattg ccttttcgac
     2461 gtgtgcctct ctggcggcgt ctaagatggc ctccgaaaaa ccggagaggc tctgcggtac
     2521 ggtgacagct tgaagactca cagtcaccta caatccgaat atacaagggc gggattcatc
     2581 ccgcgtcttg aaaacggaat cgcagcacag atgctgtact cttgtttctc caacggcaga
     2641 ggagacccgt aaacgatggg tatctcggct gtcccctcag tgccagttgc tctccatcac
     2701 gccccacttg cgacagcgga caatctctcc ggcgcttgtg gctccgttgg ttgggtcata
     2761 gaggacatgg caggtgtaga gaatggagtc ggttgtctct ggaggcccat agctcgtgtc
     2821 cggaccgtag ctggcaagca tccattcacc aaagcccaca aaacggtcgg taccgttcat
     2881 gtacgccatg ccccagtctg gatgatggtt gcaggcgatg taactgccgg taaactccca
     2941 atactcatat cggcttaccg tgtcccggtc ccagccggac agcggcggga tgtttcgatc
     3001 gcggaaggga tcgtacattc ctgaggcact tgccagatat ccgatgggcg ttgtgagaca
     3061 gtcttgaatt cgctcgcagt tgtcccctgg atagtgtgga tctcggggtg aatccaacgg
     3121 gaacctgttg ttgtctacac agtaagtctc cagtgcacca gccaacgttg ccaagtccgc
     3181 cgtgacacgg gcaacttttg ctcgaacctg tgcggacagg aaattcggga tggcaatcgc
     3241 ggcaaggatt cctattatcg cgacaacaat cagcagttca atcagagtga atccgccagt
     3301 gcgtgttctc atcaagacct ccatggtgag agcgcaaaac caacgttgtg ctcaacatcc
     3361 atgaataagg acgcagaatc tcgacataag gtttaggttt ggagatcacg actggccttt
     3421 cttcggatac ttcacgctcc agagagtcag cccttgcggg ggggcggtag ggcca
//
LOCUS       DAOQUC010000250         3474 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_30935_length_3474_cov_3.572975, whole genome shotgun sequence.
ACCESSION   DAOQUC010000250 DAOQUC010000000
VERSION     DAOQUC010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3474
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30935_length_3474_cov_3.572975"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..304
                     /locus_tag="PKH07_05540"
     CDS             <1..304
                     /locus_tag="PKH07_05540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34447.1"
                     /translation="AMRDKIDGYRASGDFDAARKLLEEAKVFTDEVVQHMTCVAELNQ
                     GLIDAKEVPGFITANIKGWLEKEQEAIDKRFVGSSLGSEPIPDSMTTLPAEVMEQR"
     gene            complement(571..1914)
                     /locus_tag="PKH07_05545"
     CDS             complement(571..1914)
                     /locus_tag="PKH07_05545"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HNT34448.1"
                     /translation="MTVNREELKKEVLRRAKEEGVRFIRLWFTDILGFLKSFAIMIDE
                     LEGALEEGMGFDGSSIEGFARIDESDMLALPDPSTYTLFPFMGPEETTVARMFCDIIE
                     PEGTPYKGDPRWVLKKNLKDAADMGFTFYVGPELEFFYFKSAEGRPEVLDRGGYFDLT
                     PLDVASALRKQTCLCLEAMGIPVEYSHHEVAPSQHEIDLRYADALTMADSTMTYRFVV
                     KEVAIQRGCHATFMPKPIAKENGSGMHVHQSLFTGERNAFFDVKNPYHLSKVAKHYIA
                     GLLKHAPEITLVCNQWVNSYKRLVPGYEAPVYITWAQRNRSDMIRVPMYKPGKEKATR
                     IEYRSPDPACNPYLAFSVMLAAGLKGIREEYPLCEPVEENVYTMPLERRKQLNIKQLP
                     EDLWEAIQVAESSQLLRDALGEHVFEKFIENKKIEWEQYRVQVTDYEIDKYLPIL"
     gene            complement(1936..2994)
                     /locus_tag="PKH07_05550"
     CDS             complement(1936..2994)
                     /locus_tag="PKH07_05550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34449.1"
                     /translation="MLPESLVHKPDFRQAVRRWEAFWQGEMIDRPCLVVTAPKEGATS
                     APHPAYMAGHTGCYREVVEQYDRWAQGIWFGAEAMPFLEPSFGPDVFAAFVGAELKYD
                     TATSWAVPFVEDWESVLPLDLTGEDWKRMKDYVQTASEFAKGRFLIGMLDLHSNMDCL
                     ASIRNPQRLCMDVLDCPELVDRAMQNVQKLYPKVFEEVYRAGSMANQGCIGWTPVYSP
                     KRFAVTQCDFICMLGPEQARRFVIPALAEEAAYLDHCVYHYDGPGALKHLDDILAIAD
                     IDCIQWVEGAGNPPLIEWMDLLKKIQAAGKSVEVRCPAEQIPIFHKNLLPDRVVYITN
                     TATQKQAEELIIWLKANS"
     gene            3344..>3474
                     /locus_tag="PKH07_05555"
     CDS             3344..>3474
                     /locus_tag="PKH07_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010527327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HNT34450.1"
                     /translation="MAKRDPYEVLGVSRNTSAEEIKKAYRQLALKYHPDRNPGDKEA"
BASE COUNT          780 a          935 c          926 g          833 t
ORIGIN      
        1 cgcgatgcgt gacaaaattg acgggtatcg tgcctctggt gatttcgatg cggcgcggaa
       61 actgctcgaa gaggctaagg ttttcacaga tgaagtggtt cagcacatga cttgtgtggc
      121 cgaactgaac caaggattga tcgatgcgaa ggaagttcct gggttcatca cggcgaacat
      181 caaaggctgg ctcgagaaag aacaggaagc cattgacaag cgctttgtcg gttcatctct
      241 cggttccgaa cccattccgg attcgatgac cacactgcca gcagaggtca tggagcagag
      301 gtagtattgc gttctgagag tggagccgcc ggtaggatgg gtcgtgaccc atcagttgtc
      361 tgatatcggc aggttcgtag tgtgcccgta agggcatatt ccggatcacg ccttacggcg
      421 taactacaaa caaggacgcg gcataaatgc cgccctacaa ctgtaacgct gattcacgct
      481 gtaggggcga ttcatgaatc gcccctacat ctcataagac aactgatgcc gggtgccctc
      541 tctctctgga aacccgcaat gcgttgcttc ctagagaatc ggaagatatt tatctatctc
      601 gtagtcggtg acctgtactc ggtactgttc ccattcgatc ttcttgtttt cgatgaactt
      661 ctcgaagaca tgctcaccga gagcgtcccg aagcagctgg ctggactcag caacctggat
      721 cgcttcccag agatcttccg gcagctgctt gatattgagt tgctttcggc gttccagagg
      781 catggtatag acattttcct caacgggctc gcacagagga tattcttccc gaatgccttt
      841 gagtcctgct gccagcatca ctgagaaggc aagatagggg ttgcacgctg ggtctgggga
      901 tcggtactcg atgcgcgtgg ccttctcctt gcctggttta tacatcggaa cccggatcat
      961 gtccgagcgg ttgcgttgtg cccaagtgat gtagacaggt gcttcgtagc ccggaacaag
     1021 gcgcttgtag gagttgaccc actggttgca aaccaaggta atctccggag cgtgcttgag
     1081 cagtccggca atgtagtgtt tcgccacttt ggagagatgg tagggatttt taacatcgaa
     1141 gaaggcgttt cgctctccgg taaagagcga ctgatgcaca tgcatgccgc tgccgttctc
     1201 tttggcgatg ggtttgggca tgaaggtggc atggcagccg cgttggatcg ccacttcctt
     1261 cacgacaaag cgatatgtca tcgtgctgtc tgccatggtc agcgcatcag cgtagcgcag
     1321 gtctatctcg tgttgactgg gtgccacttc atggtgactg tattccacgg gaatccccat
     1381 ggcttcgaga cacagacagg tctgctttcg aagtgcgctg gcaacgtcca gcggtgtcag
     1441 gtcaaaatac ccgcctcgat ccaggacttc cggacgtcct tccgcgctct tgaagtagaa
     1501 aaattccaat tcggggccga catagaaagt gaagcccatg tccgcggcat ctttgaggtt
     1561 cttcttcaga acccagcgcg ggtcgccctt gtagggtgtg ccctcaggtt caatgatgtc
     1621 gcagaacatt cgcgctaccg tggtttcttc gggccccatg aatggaaaga gcgtgtaggt
     1681 ggaagggtcg ggcagcgcga gcatgtcact ctcatcaatt cgcgcgaagc cctcaatgga
     1741 tgaaccgtca aaacccatcc cttcttccag cgcgccttcg agttcgtcaa tcatgatggc
     1801 aaaacttttc agaaagccca gaatgtcggt gaaccaaaga cggataaacc gaacgccctc
     1861 ttccttagct ctcctcaaga cttctttctt caactcttct ctgttcacag tcaacacctc
     1921 ccgcgggttt tgattctacg aatttgcctt cagccaaatt atcagctctt ccgcctgctt
     1981 ctgtgtggcc gtattcgtaa tgtagaccac tctatcagga agcaggttct tgtggaagat
     2041 cggaatctgc tccgcagggc agcgaacttc cacgctcttg ccggcggctt gaatcttctt
     2101 gagcaaatcc atccattcga tcaggggtgg atttcctgcc ccctcgaccc attggatgca
     2161 gtcaatatcc gcaatggcca gaatatcgtc gagatgcttg agcgctcccg ggccgtcgta
     2221 gtgatagacg cagtgatcca ggtacgcggc ttcttcagcc aaagcgggga tgacaaagcg
     2281 tctcgcctgt tccggtccca acatacagat gaaatcacac tgtgttaccg cgaaccgttt
     2341 gggcgagtac accggtgtcc agccgatgca accctgattt gccatcgagc ctgccctgta
     2401 aacttcttcg aaaaccttcg gatagagttt ttgaacgttc tgcatggcac ggtcaaccag
     2461 ctcgggacag tccagcacat ccatacacag ccgttgcgga tttcggatgg aggccaggca
     2521 gtccatgtta ctgtgcaagt ccagcatccc gatcaggaaa cgccctttcg caaattcaga
     2581 ggcggtctga acgtagtcct tcatgcgttt ccagtcttca ccagtcaagt ccaaaggcaa
     2641 aacagactcc cagtcctcga caaaagggac tgcccaactg gtggctgtgt cgtatttgag
     2701 ctcggcaccc acaaacgcag cgaagacgtc gggaccgaaa ctcggctcca gaaatggcat
     2761 tgcttcggca ccaaaccaaa tcccttgtgc ccatcggtca tattgttcta ctacttcccg
     2821 atagcagccc gtatggcctg ccatatacgc cggatgaggc gcggaagtcg ccccttcctt
     2881 gggtgcggtg acgaccagac agggtcggtc gatcatttcc ccttgccaga acgcctccca
     2941 gcgtcgaacc gcttgtcgga agtccggttt gtgaaccagt gattcaggga gcattgccca
     3001 agatgcctct agtgtcctct ttgtcctcca gcgttccaat aaatccgtga aggatgaggg
     3061 gatttgagca caaagcacgg tgtttttaca gctgttttct cactgcagga ctggagcaaa
     3121 gcaatagaag ctgccgtttg gcacctgccg ttgacagaat gattcttctt atcctataac
     3181 agaatccctt ccttccagaa gaagcgaagg cttgcgtcgc aggcgtggat cgtccgtttc
     3241 gatgcactgg actgttccgc cgcatcgcgt acggcgcaag gagaggctgc atgtctccaa
     3301 agtacagtgg gtcttgtccc acggatggat aggtgacttt tcgttggcaa aaagagatcc
     3361 ctatgaagtg cttggcgtga gccgcaacac ctcagccgag gaaatcaaga aggcttaccg
     3421 acagttggct ctgaaatatc atccggatcg aaatccgggt gataaggaag ccga
//
LOCUS       DAOQUC010000251         3471 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_30982_length_3471_cov_3.356557, whole genome shotgun sequence.
ACCESSION   DAOQUC010000251 DAOQUC010000000
VERSION     DAOQUC010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3471)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3471)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3471
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30982_length_3471_cov_3.356557"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..565)
                     /locus_tag="PKH07_05560"
     CDS             complement(<1..565)
                     /locus_tag="PKH07_05560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34451.1"
                     /translation="MKYFVSLLSLLVLFAVSSWADPADFTFERSIPTAPGLYPLAVDS
                     NGGLYFGNIRGASTALYYIADPVNDATNIYMIEEFNMTAGRGITGVDVDSADNVYFTA
                     DDGPDFPVLFKKYGPAPTFVPSAAFVPSATRQQGVAMIGDGVIVGTKFPEAVFFDSTT
                     GATLHTTVTNAMYQRDVAYNPHTQDIYI"
     gene            1165..2190
                     /locus_tag="PKH07_05565"
     CDS             1165..2190
                     /locus_tag="PKH07_05565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34452.1"
                     /translation="MRIELILGLLMTITAAAETQSISEQLFDDNPGHYNYCPSYLRTH
                     FGTRWIFYCANENPKEVIDSICFRRKSPFGGWHPRQIALAPSAEGWDCVHVCDPDVVR
                     GSFRDGKDCYRYALFYLGCDRLDCTHNQLGLAVAKRLSGPWKKWHNNPLITAEQDGFW
                     GVGQPSAVSLDGKGKVLLFYTRGTAVATGMFVSEVDLSDLSRVALGEEIPLITDGLTE
                     RDERGIILNNGAVALDSKERILYLVRDRHPSDPEVPHWISTEVQVAAIPLADLLSQRG
                     AWNVVANVTPSLTGKPRNHNACILRDLYGFLPNRHKLEIAFSVSDLGDDWLWTYRIWS
                     TQLRPSR"
     gene            2407..2494
                     /locus_tag="PKH07_05570"
     tRNA            2407..2494
                     /locus_tag="PKH07_05570"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2441..2443,aa:Leu,seq:caa)
     gene            2773..3312
                     /locus_tag="PKH07_05575"
     CDS             2773..3312
                     /locus_tag="PKH07_05575"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNT34453.1"
                     /translation="MEKEMQFKEMLDTNKDRIYRICCCYVRERHTREDVFQTVLLNLW
                     EGLDGFQGRSQMNTWVYRVTVNTCLGYLRAGQSRQKLLQKAAQESASDCMNSEDVPCD
                     QPTDQQDDVGRLLDCINLLQPTDRMLISMYMEDASANEIAEVLGISPVNARVKLHRIK
                     RTLKDMVERRSHGYRSDEK"
BASE COUNT          892 a          892 c          908 g          779 t
ORIGIN      
        1 gaatgtatat atcctgcgtg tgtgggttat atgcgacatc ccgctggtac attgcattcg
       61 taaccgtggt gtgcagtgtg gcacctgtag tggaatcaaa gaagaccgcc tcagggaact
      121 tggttccaac aatgaccccg tccccaatca tggcaacgcc ctgctgacgt gttgcagatg
      181 gcacaaaagc ggcgctgggc acaaaagttg gtgcaggacc gtacttcttg aaaaggacgg
      241 gaaagtccgg accgtcatcg gctgtaaagt aaacattatc cgcagaatcc acatcaacgc
      301 cagttatccc acggcctgcg gtcatgttga actcttcgat catgtagatg ttggtggcat
      361 cattgactgg atcagcgatg tagtagagcg ctgttgatgc accgcgaatg tttccgaaat
      421 acaaaccacc attcgagtca acggccagcg ggtacaaacc cggagccgtt ggaatggacc
      481 gctcaaaagt gaaatctgct ggatccgccc agctcgacac ggcaaataga accaagagac
      541 tcaagagaga aacaaaatac ttcatgacat acatcctcct actgataacg tgaaaaagat
      601 atctaaggcc aaaactcaga tttttcgact ctgtcctcct ttctcgatag tgcttctgtt
      661 gcagttttgt gctggtcaga gaggccaatg cataacgtcc gatgagcgtg gtttccagat
      721 cagaaacccc tctcctcaga cctctcccgc tagcaacaga gggcgcacgc ccttcttgca
      781 acgcactccc tacaacggca acaatatatc acatcttcga tgaaagtgaa gcgaagaata
      841 ttccgagaca agcgcaggtt ctcatgcttc aaagggattt ctcactttat gcgcagaatc
      901 gaaggactca ggacaggctg cacggttttc cgagcaaatt cacgcagaat gcaacgagtc
      961 ggcaagttcc accgatccca acgttttcag tggcctggcg attttcgttg tatgcagata
     1021 tcaccgctgc aatactatgt ccatggcaag gtatgcgcgt tcctggattc ccgtcaagac
     1081 cgccttcttt gcggttgcgg tacgttccat cgaagccgat ggaaacgtac cccaggcctc
     1141 tgccgaacga gggacataat cctattgcga attgaactga ttttaggtct cttgatgacg
     1201 ataacagcgg cagccgagac acagagcatt tcagagcagt tgtttgacga caaccctggg
     1261 cactacaact actgcccttc ctatcttcgg acacactttg gcacacgatg gatattctac
     1321 tgtgccaacg agaatccgaa ggaagtcata gactccatct gtttccgaag gaaatcccct
     1381 ttcggtggat ggcatccgcg ccagatcgct ctcgcgcctt ctgctgaagg ttgggactgc
     1441 gttcatgtgt gcgatcccga cgtagtgaga ggatcctttc gtgatggcaa agactgctat
     1501 cgttatgcgc tcttctatct gggctgcgac cgattggact gcacgcacaa ccaactcgga
     1561 ctggctgttg ccaagcgact cagcggcccg tggaagaagt ggcacaacaa tccactcatc
     1621 acggccgaac aggacggctt ttggggagtg gggcagccaa gcgcagtgag tttggacggc
     1681 aagggcaagg tgttgctctt ctacacgcga ggcacagcag tcgccacggg gatgttcgtg
     1741 tcagaggtgg atctctccga tctcagtcgg gttgctttgg gtgaagagat tcctctgatc
     1801 acagacggac tcacagaaag agatgaacga gggatcattc tgaacaacgg cgctgtggct
     1861 ctcgattcca aagagcgtat tctgtacctc gtccgtgaca gacatccgtc tgaccctgaa
     1921 gttccacact ggatttcaac tgaagtgcaa gtcgccgcaa ttcccttagc cgatttgctg
     1981 tcgcaacggg gcgcttggaa tgtggttgcg aacgtcacgc cttcactgac cggcaaaccc
     2041 agaaaccaca atgcctgtat tttgcgagac ctctacggtt tccttcccaa tagacacaaa
     2101 ctggagatcg ccttctcagt ttccgatctt ggggacgact ggctttggac gtatcgcatc
     2161 tggtcaacac agctgaggcc atcgcgctaa ttgctgatgt tgtggtgcgg gcttcctgcc
     2221 tgcatccgat ccggagggag tttcgcaggt cagcactcat gctgcgcggg acaaatcccg
     2281 ccatacaact ggtgtttcct gcctccggca gaacgaagcc agtaccccca tacgcaatac
     2341 gcaacgcaac ttgatcttga aatccgtgta aattcagata tcatccgctt tggttgtttc
     2401 gccagtgccg gagtggtgga actggcagac gcgcgggact caaaatcccg tgaccctttg
     2461 ggtcgtgagg gttcgacccc ctcctccggc accatcgcga aacacgtcgg gaatcatcca
     2521 ccgatcaact ctttttcggc aatcaaagaa tacctcccgt caaagcactt catcttgctc
     2581 ttgagggctc cactgcattt ggagacacac aagaagacgc atgcagtggc aacagcattg
     2641 tgtgcgaact aagaacaggg gcggtgtacg taggttgatg cggatatcat ccacgacact
     2701 ttcatccgat gcgacatttt ctgtaacata ttgccacctg atgcatctaa atgagcagaa
     2761 ggaagaagaa agatggaaaa agaaatgcag ttcaaggaaa tgctcgacac aaacaaagac
     2821 agaatttaca ggatctgttg ctgttacgtt cgagagcgac acactcggga agatgtgttc
     2881 caaacggtgc tgcttaacct ctgggaggga ctggatggat tccaggggcg ttcacagatg
     2941 aacacttggg tctatcgagt gaccgtcaac acatgtctcg gatacctgcg tgcaggacag
     3001 agcagacaga aactgctgca aaaggctgct caagagtcgg cctccgactg catgaacagc
     3061 gaggatgtgc cgtgtgatca acccacggac caacaggatg atgttggcag gcttttggac
     3121 tgtatcaatc tgttgcagcc cacggaccgg atgttgatct ccatgtacat ggaggacgcc
     3181 agtgccaatg agatcgccga agtactgggg atctctccag tcaacgcccg ggtgaaactg
     3241 catcgaatca agagaacact caaggacatg gtggagagga gaagtcatgg atatcgatca
     3301 gatgaaaaat gagatgaaga aggtgaaagc gccgaagatg aattcctcca acaatggaga
     3361 ttctcacggc gagttcgaga gtttgatcgc gcgcctgaag gctgcggaag caaaagagca
     3421 gaagagtctg agacaagggc aaatcttgtt ccggtatgcc atggtgttca t
//
LOCUS       DAOQUC010000252         3471 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_30984_length_3471_cov_3.100703, whole genome shotgun sequence.
ACCESSION   DAOQUC010000252 DAOQUC010000000
VERSION     DAOQUC010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3471)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3471)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3471
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30984_length_3471_cov_3.100703"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2678)
                     /locus_tag="PKH07_05580"
     CDS             complement(<1..2678)
                     /locus_tag="PKH07_05580"
                     /inference="COORDINATES: protein motif:HMM:NF019236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HNT34454.1"
                     /translation="MIRAARGSVVWVVWAVIVAVAGYLTVSLCIHDSQADQYLSGQTP
                     DAPSIGEYSALNQWLNWRETLSQPQLKDITAVYTRTDATICDSGDTLLSLPPLTDVDC
                     VGVVDPFNIGEENPYLSLEYQDYGSSTWQTLMSEDLATVESGCPGRTIAYRSWTVDFA
                     DTQPGRSFRWKAEQEKDIVIYSTPFQVYTPTDTPSPESPTITPSFTPTISNTPTQTPT
                     GPPTDTPVGTPTNSPTPTISNTPTRTPTPTIKLSGGSLFVMQSLDPTPNPTLDIDVVI
                     PGQNIWLMWPGVNDPYRPGAPNFTDIIVDPMTQCANGTASLFNLPQMLTYNGEPYFGV
                     LLNETDYRSALPSVVLAGQSIGHTIAWRFAGEDDLYFSDCGPAQPGYRGGIRWATVTP
                     TGMTTETPTDTPTPEIPPTETPTSTVTKTPTHTPTWTFTATITPTPSPTFKVIGFQGH
                     YVFDQDQIIPEGQAWVIGSGSILEFVDADDPYRNLGFDPSKVELIVRGALIADGTQGT
                     IIFRRAMATPTFTPSYTPTSTATRTPPPTPTATFTPSRTPTQTPTQTPTPLDVGLVFY
                     AVGTAGEAAVISVNQGVLRTFSGLEPTDLESLSWWDDPANPGEMVLVGGRSGASTVGE
                     AWAIDVSDPLAPGAQPSGMLLFDLNTSIRGMAKSPESESIYISAPISDASPNQFELKT
                     WDRNSGTTTPIGVIQDEATGEDLIVRGLCFPPGSNALYGIGPVSSDYPTSWRLVRFQN
                     PATSALATTVATGDRAVNQSLVIAADSTGTRYFALGPRRFAELLREKTEEGDTIWSLS
                     PTVNYTFPDQFNLRGLEVITLPEPTPTPSPSPTWDGRDHIWVSQATGNDLPSTQGTET
                     DPFKTVTYAINARSSSPNPVFVHIKEGNYSEATGEL"
     gene            complement(2848..>3471)
                     /locus_tag="PKH07_05585"
     CDS             complement(2848..>3471)
                     /locus_tag="PKH07_05585"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNT34455.1"
                     /translation="IILANAEVLLDRVRDDEQREMLTDLAQEANQLEHMVQAALDRMR
                     SDSEHSERFSPFEVVRECADQFRRSLPDSRIEVVVKADSQLSDGDSVCARGERIKIEG
                     AIMNLLRNSREAMPEGGTIEVGVEVEEGEKTIRIYVRDNGPGISRYAIRRVFLPFYSE
                     KEKGTGLGLPTVQRIVREFGGEVAVKTRKGRGTEVSLVIPREMLASK"
BASE COUNT          757 a          952 c          963 g          799 t
ORIGIN      
        1 aagagttctc cagtcgcttc gctgtaattc ccttctttga tatgtacgaa gacggggttc
       61 gggctgctgg agcgagcatt gatggcgtag gtgaccgtct tgaaaggatc ggtctctgtt
      121 ccctgtgttg aaggaagatc gttgccggtt gcctgactga cccagatatg atcacgtcca
      181 tcccaggtcg gggacggtga cggcgttgga gtcggttccg gcagcgtgat gacttcaaga
      241 ccgcgaaggt tgaactgatc agggaacgtg tagttgaccg tcggactcag gctccagatc
      301 gtgtctcctt cttcggtctt ctcccgaagc agttcagcga accggcgagg cccgagagca
      361 aagtaccggg ttcccgtcga atccgctgca atcacgagcg actggttcac agcacggtct
      421 cccgtagcca ccgtcgtagc cagagcagac gtagcgggat tctggaaacg aacgagcctc
      481 caggacgtcg ggtagtccga cgagacgggg ccgatgccgt agagcgcatt ggatcctggc
      541 gggaagcaca gacctcggac gatcaagtct tctcccgtcg cttcatcctg gatgacgcct
      601 atcggagtgg ttgtccctga gtttctgtcc catgttttca gttcaaactg gttcgggctg
      661 gcatctgaga tcggagcaga gatgtaaatg gattcggatt ccggagattt cgccataccc
      721 cgaatgcttg tattcaagtc aaacaagagc attcctgaag gctgggctcc aggcgccagc
      781 ggatcagaaa catctatcgc ccatgcttcg ccgaccgtgc tcgcaccgct gcggcctccc
      841 accaacacca tctctccagg attagcggga tcgtcccacc aggaaaggga ctccagatcc
      901 gtgggttcca aaccgctaaa cgtccgcaac acgccttggt tgacgctgat aaccgcggcc
      961 tctcccgctg tacccaccgc atagaacacc agaccgacat ccagaggtgt cggagtctga
     1021 gtcggcgtct gagttggcgt acgcgatgga gtaaaggttg ctgtgggtgt cggaggaggc
     1081 gtccgcgtcg ccgtagaggt cggagtataa ctcggtgtga aagtcggcgt cgccatggca
     1141 cggcgaaaaa tgatcgtccc ttgcgtgccg tcagcgatca gagcaccccg aacaatgagt
     1201 tccactttgg agggatcaaa gccaaggttt ctgtagggat cgtcagcgtc cacaaattcg
     1261 aggatactgc ccgagccaat cacccatgcc tgaccttcgg gaatgatctg atcctggtcg
     1321 aagacataat gtccctggaa tccaatgacc ttgaaggtgg gcgacggcgt tggtgtaata
     1381 gttgccgtga acgtccaagt tggagtatgg gttggggtct tcgtgaccgt cgaagtcggc
     1441 gtctctgtcg gaggaatctc cggagtcggc gtgtccgtcg gcgtttcggt tgtcatgccg
     1501 gtcggcgtga cggtcgccca tcggataccg ccccgatacc ccggttgtgc cggcccgcag
     1561 tcgctgaaat acaggtcatc ttcaccggca aagcgccagg cgatggtatg cccaatggac
     1621 tgaccggcaa gaaccaccga aggcaatgca cttcgatagt ctgtttcgtt caaaagcaca
     1681 ccaaagtaag gctctccatt gtaggtcaac atctgcggca agttgaacag agaagcggtt
     1741 ccattggcgc actgtgtcat cggatccaca atgatgtccg taaaattggg ggctcccggg
     1801 cgataggggt cattgacgcc aggccacatc aaccagatat tctgtcctgg aattaccaca
     1861 tcaatatcca gagtcggatt tggtgtcgga tcgaggctct gcatgacaaa aagtgaacct
     1921 cccgagagct taattgtcgg tgtcggcgtt cgggtgggtg tattggagat ggtcggtgtc
     1981 ggggaattcg tcggggtacc gacgggagta tccgttggag gcccggttgg agtctgcgtt
     2041 ggtgtattgc ttatcgtcgg cgtgaacgaa ggtgtgatgg tcggactttc gggacttgga
     2101 gtgtccgttg gagtgtagac ctggaacggc gtgctgtaga taacaatgtc cttctcctgc
     2161 tccgctttcc atcggaagga gcgcccaggt tgtgtgtccg caaagtcaac cgtccatgaa
     2221 cgataggcga ttgtgcgacc tggacaaccg gattcaacgg ttgccagatc ctcagacatc
     2281 agcgtctgcc aagtggaact gccatagtcc tggtattcca aactcagata tggattctcc
     2341 tcaccgatgt tgaagggatc aacaacccca acgcaatcca catcggtgag tggtggaagg
     2401 ctcagcagag tatcaccact gtcacagatc gtagcatcgg tgcgtgtgta aacagctgta
     2461 atgtctttca actgaggttg tgagagtgtc tcacgccagt tcagccattg gttgagagca
     2521 ctgtattcac caatggatgg cgcgtcaggc gtttgtcccg acaaatactg gtctgcctgg
     2581 ctgtcgtgaa tgcaaaggct gactgtcaga tatcctgcca cagccacaat aacagcccac
     2641 acgacccaaa ccacgctccc gcgtgctgct ctaatcatgt ttggattctc ctcaacgaat
     2701 gttttccggt gattagtgtt cacatcaatg cctctcccac cctcacgaag tggggctaca
     2761 tgaattcatc aaaaatctaa caaatatggg tgggcgttgt caacctaaaa tccgaatttc
     2821 gtttgatttc tgtcttctga atgaaggtca tttcgaagcg agcatctccc tgggaatgac
     2881 cagtgaaact tccgtccctc ttcctttgcg tgtcttgacc gcaacttcgc cgccgaattc
     2941 acgaacaatg cgctggacgg taggcagtcc caaaccggtg cctttctcct tctcagagta
     3001 gaaaggaagg aagactcgcc gaatggcata tctggaaatg cccgggccgt tgtcccgaac
     3061 atagattcta atggtcttct ctccttcttc cacctcaact ccaacctcaa tcgttccccc
     3121 ttctggcatc gcttcccgtg aattgcgcag gagattcatg atcgctccct cgattttgat
     3181 gcgctctccc cgcgcacaaa cggaatcccc atcggacagt tgggaatcgg ccttcacgac
     3241 gacttcaatt ctcgagtctg gcagactgcg cctgaactga tccgcacatt cccgaaccac
     3301 ctcaaaaggc gagaaccgct cggaatgctc cgaatcgctc cgcatcctgt caagcgcggc
     3361 ctgaaccatg tgctccagct gattggcctc ttgagccaaa tcggtcagca tctcgcgctg
     3421 ctcatcgtcc cgaacgcggt caagcagcac ttcggcgttg gccagaatga t
//
LOCUS       DAOQUC010000253         3464 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_31078_length_3464_cov_3.384570, whole genome shotgun sequence.
ACCESSION   DAOQUC010000253 DAOQUC010000000
VERSION     DAOQUC010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3464)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3464)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3464
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31078_length_3464_cov_3.384570"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1475)
                     /locus_tag="PKH07_05590"
     CDS             complement(<1..1475)
                     /locus_tag="PKH07_05590"
                     /inference="COORDINATES: protein motif:HMM:NF017263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HNT34456.1"
                     /translation="MQSVAGAAQQITCNKEPNPAMDIHFPLFSFAYKAAVLHHVETVL
                     FFWAVCLSLLTLSPNAHSVERPESVVPSVQEHPYLFITRSDIPDIMARVEKHDWAKKT
                     YEGILENAERLLEEDLIPPPSTGRHSMVYMCPDCGGLLKTLSPTEHQCKDCQKIHTGM
                     PFDAVLYKSQHGKLENAAMTLGLASLFTGKDVYARKALEILLKYADVYDTYPMINPYG
                     EPRANGARIYDQTLNEAIWLVSISWAYDLILATDVATTEEKRHVEEHLIRPSVMTVRK
                     NPSNRISNWQTWHNAGMLAAGLCLRDQEIIDHALLADGGYFYQLEHSIKEEGMQYEGS
                     WSYHFYALHAFLKTAEMGHKSGLNTYGHEPLRRALLLPLQMAMPNGKLPAIHDGTEWA
                     PSTTYYEYAAARYDEPAFEDFLAQHKRVGWEALLMGIDDPQPHPTPLQSVVLNDSGLA
                     VLRRGGSYLLLDFGEHGGGHGHYDKLNVVYFTQGSVVASDPG"
     gene            complement(1504..2451)
                     /locus_tag="PKH07_05595"
     CDS             complement(1504..2451)
                     /locus_tag="PKH07_05595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34457.1"
                     /translation="MKIGASYMGHHNPQHIETDIKEMVSLQMDDVLLAAQENDFIHYP
                     GKLQLTARIAKDHGIRPIIIFWGALNVFGGGRMSHFLLSHPEGFQVAKDGAHLPHGCY
                     VNPVCVGRIQEMIDISAKYGFEGYFVDEPSPMRECFCASCQKKFREISGEDLLRAQPE
                     MLERFRAQCVIDYVRHISDYCKANHPNMRTMCCLMPCDRSMWKACSEIKNLDEIGTDI
                     YWINNDEDVENMRPLIRELDCFAKTQAKTHQEWLQCWDVRAGRENRIVEQGKVILQEP
                     ADALYVWAWKGQIGTTESCDDPLAAWARVCEVFRMAKQK"
     gene            complement(2448..>3464)
                     /locus_tag="PKH07_05600"
     CDS             complement(2448..>3464)
                     /locus_tag="PKH07_05600"
                     /inference="COORDINATES: protein motif:HMM:NF025681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34458.1"
                     /translation="LISILEAANEMTQGKRMITCGGFVPWWLKHCRANWAGRNPDTSK
                     HDAVPAEWRFVDILTAHNGMLDADAYGLTGLTNASVYQHCKLRDKYLQSHSGSEKTYD
                     PKKTYVLFGMLDYDSAAWLAQAIPANWDDPNRGKVPLWWGFNPILSDRVPMVFDHVYS
                     TLTPNDTVGADEGLGYVNPNLLEGDRKFSTLPPAGEMYLQHAKPYFERFSMTTTVFVI
                     VGDQVPVTERTLQILAETSPDGIGFQGGDRVRDGVYFGTAFKQEEQDWFYAMKPEETA
                     KALEHFIRQKGAGGFVFFRCILFSPTQIVKAVEQVRQQAPDLDFEVLDPVTYFRFMKK
                     EKQP"
BASE COUNT          771 a          940 c          897 g          856 t
ORIGIN      
        1 cctggatcgg aggcgaccac actgccttgt gtgaagtaga caacgttcag cttgtcgtag
       61 tgcccgtgcc cgcctccatg ctcaccgaag tcgagtagaa ggtaactgcc accccttctg
      121 agaacagcca agccggaatc gttcagaacg acgctttgca gtggggtggg atgcggttga
      181 gggtcatcaa tgcccatcag aagcgcttcc caaccgactc gtttgtgttg cgcaaggaaa
      241 tcctcaaatg ccggttcatc gtaccgagcg gcggcatact catagtatgt ggtcgagggc
      301 gcccactccg ttccgtcgtg aatcgccggg agttttccat tgggcatcgc catctgaagc
      361 ggcagaagca gtgctctgcg aagcggctca tgtccatacg tgttcaagcc ggacttgtgc
      421 cccatctctg ccgttttcag aaaagcgtgc agtgcataga aatgataact ccaggaacct
      481 tcatactgca tgccttcctc tttgatgctg tgctcgagtt ggtagaagta cccgccgtcc
      541 gccagaagcg catggtcaat aatctcctgg tcgcgcaaac aaagacccgc cgcaagcatt
      601 ccggcattgt gccaggtctg ccagttgctg atcctgttgg atggattctt tctgacggtc
      661 attactgagg gacgaatcag atgttcctca acgtgtctct tctcctctgt tgtggcgaca
      721 tctgtggcga gaatcagatc gtacgcccat gaaatcgaga ccagccagat tgcctcgttg
      781 agagtctgat cgtaaatgcg cgccccgttg gcgcgaggct ctccatacgg gttgatcatg
      841 ggataggtgt cgtagacgtc tgcgtacttc aggagaatct ccagcgcctt tcgagcgtac
      901 acatccttgc ccgtgaacag cgaggcaagc cccaaggtca tcgcagcgtt ttcaagtttc
      961 ccatgttgac tcttgtaaag aaccgcatca aaaggcatcc cggtgtgaat cttttgacag
     1021 tctttgcatt gatgctcggt tggagaaaga gtcttcagaa gtcccccgca gtcaggacac
     1081 atgtaaacca tcgaatggcg tcccgttgat ggtggtggta tcaagtcttc ttcgagaagt
     1141 ctttcggcgt tttcaagaat tccctcatag gtcttcttcg cccaatcgtg tttctcgaca
     1201 cgtgccatga tgtccggaat gtcggaacgt gtgatgaaca gatacggatg ctcttggacg
     1261 ctgggaacca ccgactccgg tctctctact gaatgggcgt tcggcgagag tgtcaacagg
     1321 ctcaggcaga ctgcccagaa aaaaaggact gtctcaacgt gatgtagcac agcggctttg
     1381 tacgcgaatg agaatagggg aaaatgaata tccattgcgg gattcggctc cttgttgcag
     1441 gtaatttgct gtgccgcacc ggcaacactc tgcatcgggc gtgtcagttc ctgacacgca
     1501 ggctcatttc tgttttgcca ttcgaaagac ttcgcagact cttgcccagg cagccagcgg
     1561 atcgtcacaa ctttccgtcg tgccgatctg acctttccat gcccagacgt agagtgcatc
     1621 ggcaggttcc tgaagaatga ctttcccctg ttccacaata cggttttctc gaccggctcg
     1681 gacatcccag cactgaagcc attcctgatg cgtcttggct tgtgtcttgg cgaaacaatc
     1741 caactcgcgg atcaatgggc gcatattctc gacgtcttca tcgttgttga tccaatagat
     1801 atccgttcca atctcgtcga gattcttgat ctcggaacag gctttccaca tgcttcgatc
     1861 gcagggcatc aggcagcaca tcgtacgcat attcggatgg tttgctttgc agtaatccga
     1921 gatgtgcctg acgtaatcaa tgacacactg tgctcgaaaa cgctccagca tctcgggttg
     1981 cgctctcagg aggtcttccc cagaaatctc ccggaacttc ttctgacaac tggcgcagaa
     2041 gcactcacgc atggggctgg gctcatccac aaagtaacct tcaaaaccat atttcgctga
     2101 gatgtcaatc atctcttgga tccgaccgac acagactgga ttcacgtagc aaccgtgagg
     2161 gagatgagcg ccgtcctttg cgacctgaaa tccttcagga tgagacagca gaaagtggct
     2221 catgcgccca ccaccgaaga cgttcagagc accccagaaa atgatgatcg gacgaatgcc
     2281 gtggtctttg gcgatcctgg cagtcaactg gagttttcct gggtagtgta taaagtcgtt
     2341 ttcctgcgct gccaacagaa cgtcgtccat ctgcagcgag accatctctt tgatgtccgt
     2401 ctcgatgtgc tgtggattgt gatgccccat ataggatgcg ccgatcttca tggttgtttc
     2461 tccttcttca tgaacctgaa ataggtgact ggatcaagaa cttcgaaatc caggtcgggg
     2521 gcttgctgcc gaacttgttc gactgcctta acaatctgcg ttggactgaa gaggatgcat
     2581 cggaagaaga cgaatccccc agcccccttc tggcgaatga aatgctccag ggctttggcg
     2641 gtctcctctg gcttcatcgc gtaaaaccag tcctgttctt cctgcttgaa tgctgtaccg
     2701 aagtagacac catcacggac tcggtcgcca ccctgaaaac cgatgccgtc cggtgaggtc
     2761 tcggcgagaa tctgcagtgt ccgttccgtc acaggaacct gatccccgac aatgacgaag
     2821 accgtggttg tcatcgagaa acgctcgaaa tagggtttgg cgtgttgaag atacatctcc
     2881 cctgccggag gaagtgttga gaacttcctg tcgccttcca agagattcgg attcacgtag
     2941 cccagtcctt catcagcgcc gacggtgtca ttaggtgtca aagtactgta cacatggtca
     3001 aacaccatgg gcacacgatc ggagaggatt ggattgaaac cccaccaaag aggaaccttc
     3061 cctcggtttg gatcatccca gttggcagga atcgcctgcg ccaaccatgc cgctgaatcg
     3121 tagtccagca ttccgaagag cacataggtc ttcttgggat cgtatgtctt ttcgcttccc
     3181 gaatggcttt ggagatactt gtctctgagt ttgcagtgtt ggtagactga agcgttagtc
     3241 agtccggtca agccgtaggc gtctgcatcc aacatgccat tgtgtgccgt gagaatgtcc
     3301 acaaagcgcc actcagcggg taccgcatcg tgtttgctgg tatctggatt tctgcctgcc
     3361 cagttcgcac gacaatgctt gagccaccac gggacaaatc caccgcacgt aatcattcgt
     3421 ttgccctggg tcatctcatt ggcggcttcc agaatactga tcag
//
LOCUS       DAOQUC010000254         3463 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_31087_length_3463_cov_3.421948, whole genome shotgun sequence.
ACCESSION   DAOQUC010000254 DAOQUC010000000
VERSION     DAOQUC010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3463)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3463)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3463
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31087_length_3463_cov_3.421948"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..849)
                     /locus_tag="PKH07_05605"
     CDS             complement(<1..849)
                     /locus_tag="PKH07_05605"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNT34459.1"
                     /translation="MDTFVVLGAGLAGLNAALHLSESTDKRVIVLEREPRVGGLAASL
                     EWKGARLDLGPHRIFTEIPEIRQLINDLVGDELIQVKRSSHMYLNNRFLAYPPRIGQM
                     ALALGPSKMIRWGLSYLAGQIPQIRRTPTDETYETFLCRQFGSALYQDFFLPFARKVW
                     GTSPSKLSSDIVKTRVASKSLLHALLGKTDKKGRPSTLREFPYPKLGIGRIAERLKER
                     LEARGVRIILESEPTLIKMSSKGEVLLAFQDRRSDQMVTAELKGVVSTIPLVELIEIL
                     RAPGWET"
     gene            complement(860..1552)
                     /locus_tag="PKH07_05610"
     CDS             complement(860..1552)
                     /locus_tag="PKH07_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018204290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNT34460.1"
                     /translation="MKTEQKLSIIIPIHNEIDLIAEVLGRVRAVEYKPKELILVDDMS
                     NDGTREYLQSEENNPNTTVLYHEKNFGKGMAIRTGLAVATGEIIIVQDADMEYNPDDI
                     LKVIQPILDGKTEVCYGSRFLGSIERMRLPNWVANRILALMVRVLYGAKITDEATAYK
                     AFRQKVIRQVELDCMGFEFCPEVTAKVLKLGYRIQEVPVTFRARTFNEGKKIGWRDFF
                     VAVWTLLKYRFV"
     gene            1732..>3463
                     /locus_tag="PKH07_05615"
     CDS             1732..>3463
                     /locus_tag="PKH07_05615"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNT34461.1"
                     /translation="MLRKILERIKQTLSGTPQEPSQRALLTEKWQQFARQNLGCSQWI
                     DLSTAEGVLVVQEMPLKDVPSSRQETVVGWVEPAHYLPDPTRIVEKAVDCRARNILVT
                     AKDPTPCNGLEVMPNLGSSVYPISLWVSQCQKKGFSSITLDFDTDGVVTILAGRRGSP
                     VPGSSPEKKPFQQVLEISEGIADKVLLAPHSGWTLADGSLASSGLGTLYAINRTSEPV
                     SLRLEIDAEADIPDSTLKVHLDFCYLQGFTLGGKTPLCVKEIILTPGGHGIQILPSDG
                     TQVKILKVQADGVSYPNDRFLLRLPCDQYQRHRVVADVVKCLAKQNRTSVLDVGGGRG
                     TLTLFLPEHQVTPLDRRAYDVVGHVRSEGERLPFGDASFEIVSSVDVLEHVPASQREG
                     FLCELWRVAKRAVILACPFHDDRVASAERLLADFAVRIRGQENAFLMEHIQNGLPDFA
                     QTQETLETFGASTVAIPNGYFARWFSMMVLRTMLEANLEHYSRLERILAAYNKGYYAL
                     DNAEPCYRRVLVACKQQEDRNRVAGFASTLIASNPTGNPSLTDPRMALAFYGIPVSEA
                     FEQWYRDSAAD"
BASE COUNT          792 a          928 c          920 g          823 t
ORIGIN      
        1 agtttcccat cctggtgccc gtagaatttc gatcaactcc accaagggta tcgtggaaac
       61 aactcccttc agttctgcag tcaccatctg atccgaccgt ctatcctgaa acgcgagcag
      121 cacctctccc tttgaactca tcttgatcag cgttggctct gactccagaa tgatgcggac
      181 tccacgcgct tcgaggcgct ctttgagtcg ttctgcaatt cggccaatcc ccagttttgg
      241 atatgggaac tctcggaggg tactgggtct gcccttcttg tcggttttcc ccagaagcgc
      301 gtgaaggagc gacttggatg ccacccgagt cttgacaata tccgatgaga gtttcgaagg
      361 agaagtcccc cagacttttc gcgcgaaggg aagaaagaaa tcctggtaga gtgcgcttcc
      421 gaactgacga caaaggaacg tctcgtaggt ctcatctgtc ggtgtgcgcc gtatttgagg
      481 aatctggcca gccagataac tgagtcccca gcgaatcatc ttcgacggac caagcgcaag
      541 agccatctgc ccaatgcgag gtgggtaggc aaggaaccga ttgttcaagt acatgtgact
      601 ggagcgtttg acttggatga gttcatcgcc aaccaaatcg ttgatgagtt gtcggatttc
      661 gggaatctcc gtgaaaatgc gatgagggcc caaatccagc cgtgcgccct tccattccag
      721 ggacgctgcc agtccgccaa cgcggggctc tctttcgagc acgatgacac gtttatccgt
      781 gctttctgaa agatgcagtg cagcattcag tccggcgaga cctgctccca agacaacaaa
      841 agtatccata tccctggctt cacacgaaac ggtacttcag caacgtccag accgcgacga
      901 agaaatcacg ccagccaatc tttttgcctt cgttgaatgt acgagcgcga aaagtaacgg
      961 ggacttcctg aattcgataa cccaacttca gaacctttgc cgtgacttca ggacagaact
     1021 caaaccccat gcagtccagt tccacctgac ggatgacttt ctgccgaaag gctttgtagg
     1081 cggttgcctc gtcggtgatt tttgcgccat agagaactcg aaccatcaag gctagaatcc
     1141 ggttggccac ccagttgggc aggcgcatcc gttcaatgct tccgagaaaa cgggagccgt
     1201 aacagacttc cgttttgcca tccagtattg gctgaatgac tttgagaatg tcgtccggat
     1261 tgtactccat atcggcgtct tgaacgatga tgatctcgcc agtcgcgacg gccaagcctg
     1321 ttcggatggc catacccttg ccgaagttct tctcatgata gagaaccgtt gtgttcggat
     1381 tgttctcctc ggactggaga tactctcgag tgccgtcgtt ggacatatca tccacaagga
     1441 tcagttcctt gggcttgtat tctacggcac ggacacgccc aagcacttcg gcaatcagat
     1501 cgatctcgtt gtggatgggg atgatgatgg acagtttctg ttccgttttc aaaatttctc
     1561 caccggccgt gaagttcctg aaatatcacc ggttctcaga ctataccgag agatgaattc
     1621 gtaatcaagc gagtatctga aacagcgcat tcacattcat cttgcattca ctgtgcccgc
     1681 gggatactct catcaggccg gacagcattc atcaacagag gtcgtttgaa catgctcaga
     1741 aaaatactcg aacgcattaa gcagactctc tccggaactc cacaggaacc ttcccaaaga
     1801 gccttactga cagagaagtg gcagcagttt gcacgacaga atcttggctg ctcccaatgg
     1861 attgacttga gcaccgctga aggggtgttg gtggttcaag agatgccgct caaagacgtc
     1921 ccttcatcaa gacaggagac tgttgtcggc tgggtggaac cagcacacta tcttcccgac
     1981 ccaactcgaa tcgtggagaa ggcggttgac tgcagagccc gaaatatcct cgtcacggca
     2041 aaggatccga ccccctgtaa cggactggag gtcatgccga atcttggttc ttcggtctat
     2101 ccgatctcgc tctgggtctc tcaatgccaa aagaagggct tcagctcaat cacactggac
     2161 ttcgatacgg atggagtcgt gaccatcctc gcggggagaa gaggttctcc cgtgccaggc
     2221 agtagtcctg aaaaaaagcc ttttcagcag gtgttggaga tctcagaggg gatcgccgac
     2281 aaggttcttc ttgctccgca tagcggttgg acactggcag acggatcttt ggcatcgtcc
     2341 gggctgggaa cactgtacgc cataaaccga accagtgagc ccgtctcgct ccgacttgag
     2401 attgacgcag aggcagatat tccggattca acactgaaag tccacctgga cttctgttac
     2461 cttcagggct tcactctggg agggaagacg cctctctgcg tgaaggagat catcctgact
     2521 cctgggggac atggcatcca gattctgccg tcggatggca cccaagtcaa gatactcaag
     2581 gttcaagcgg atggagtctc ctatcccaat gaccgatttc tccttcgtct gccttgcgat
     2641 caataccaga ggcatcgtgt ggtcgcagat gttgttaagt gccttgccaa acagaacagg
     2701 acgagcgttc ttgacgtcgg aggcggacgc gggacgctga ctctgtttct gccagagcat
     2761 caggtaactc cattggacag acgtgcctat gacgtggtgg gacatgtcag gagcgagggt
     2821 gagcgcctgc cgtttggaga tgcatcgttt gaaattgtct cctcggtgga tgtcttggaa
     2881 catgtgcctg cctcacaacg agaaggtttt ctttgcgagc tatggcgggt tgccaaacgt
     2941 gcggtcattc tggcctgtcc tttccacgat gatcgggtgg catcggcgga acgccttctg
     3001 gcggatttcg ccgttcggat acgcgggcag gaaaatgcat ttctgatgga gcacattcag
     3061 aacggtctcc ccgattttgc gcagacgcaa gaaacgcttg aaacgtttgg tgcctcgacc
     3121 gttgccattc cgaatgggta tttcgctcgg tggttctcca tgatggtgct gcgaacgatg
     3181 ctcgaagcaa atctggaaca ctacagcagg ttggagcgca ttctggccgc ctacaataaa
     3241 gggtattatg ctctagacaa cgccgagccg tgctaccgtc gtgttctcgt tgcctgcaag
     3301 cagcaggaag accggaatcg tgtcgcaggt ttcgcgtcca ccttgatcgc ctccaatccg
     3361 acagggaatc cttctctcac agatccaagg atggctctgg ccttctacgg gatccccgtc
     3421 tctgaggcct ttgagcaatg gtatcgggac tctgcggcgg aca
//
LOCUS       DAOQUC010000255         3439 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_31395_length_3439_cov_3.088652, whole genome shotgun sequence.
ACCESSION   DAOQUC010000255 DAOQUC010000000
VERSION     DAOQUC010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3439
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31395_length_3439_cov_3.088652"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2846
                     /locus_tag="PKH07_05620"
     CDS             <1..2846
                     /locus_tag="PKH07_05620"
                     /inference="COORDINATES: protein motif:HMM:NF021570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2298 domain-containing protein"
                     /protein_id="HNT34462.1"
                     /translation="HSVLDRTAVLNKIDAINLTDGSIPPSMPKAVRESERQVPEKIAQ
                     ITQCRDAWWYEVWQVIKWLAILDLLALAMLPLTCSLFRSMPDHGYPLARTLGLIVSTY
                     LVWILVNLHLGYFTVMLCWISLFALAASCWLFVTKEHLFQVIREKFSLILVTEILFLS
                     TFIIFLVIRAFNPEIFWGEKTMDFSFYNAVVRGSTFPPYEPWFSGTLLNYYYYGDILI
                     GYVTKLASTPTAFGFNLSMATIPALTVLCAFSLVYNLTKRIRWGVLGALFVGYIGNFD
                     PVYQLAIKGSLENSFNDLLRGEIVRQHGLLAGLWLSAVALAQTVVLSLAATVRAILSG
                     ALFFQPSHGRMWDSFWASSRALGEGMINEYPVWSWLFADLHAHVLVMPVSLLALSFIY
                     LAYTSRKALFENHLLLGGLFFAITIGSQLATNIWDFISYMGLFGLILFVRALVLPKKT
                     LCFLETPSSVIEGGDSDASVSFQENIVAPLFVPVCGVIGFVVAWTYLWPSICELHPYW
                     LGCINSRPVGLAIALAVVVLSTARGLVVDESAQLLSAFWKILRECAVPLIVIVGLSGL
                     LFYHFHINLHTDAATFKFNNDGYINFWNAMRHFGFFILLSLTWIFISYYSAFRGRVLN
                     KDISLPSPILPLVVGIVGSALVARQLRLSGFWLPAHGIVVYVMLLPALVSTMILWRNS
                     PTKLFISTLLFAGWTIATISELIVIIDRMNTVFKFYHHTWIFVAVGCAAAVGVIRSEW
                     MGRVFEKTKGNLLWIVHIKIVRSIFCLAFLFALFLTLICTYRAVHGTVTRNQKKSDKP
                     TLNGLNFLKHTPQEAELFEAVDWLNRNVAGVQVIAEGFTNMGYDESTIACKYTGLPTL
                     LGWPHHTKQRGHTPPELSERDQDLRQLYTTDDSASSEEICAKYNIRYIFLGHIERRLY
                     PDTETKLTNRTHLEPVFRSQSGAYIVFRVVEPS"
     gene            2909..>3439
                     /locus_tag="PKH07_05625"
     CDS             2909..>3439
                     /locus_tag="PKH07_05625"
                     /inference="COORDINATES: protein motif:HMM:NF027541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxGYxYP family putative glycoside hydrolase"
                     /protein_id="HNT34463.1"
                     /translation="MGNVLPEIVVLDLKAALSGEDERGTRWAYDAAKAIAALQGIVNR
                     RRPNLFLRYLSNGLMRERSQPTDSCSLDTYWLNWLRQSDRMLAEHTLVETDKICEVFT
                     RFADSIHGIVIWDESVPSTANVAATLAGAKNLIPLRGSTAANSFFAQFRELLPNIPVE
                     HDLRDMFTGTGKIPETT"
BASE COUNT          729 a          954 c          844 g          912 t
ORIGIN      
        1 atcacagcgt cttggatcga actgcggtgc tgaacaaaat tgacgcgatc aatctgaccg
       61 acggctccat tccgccatcg atgccgaaag ccgtcagaga atcggaacgg caggtaccgg
      121 agaagatcgc acagatcacc caatgccgcg atgcgtggtg gtatgaagtc tggcaggtga
      181 taaagtggtt ggcgattctt gatcttctgg cattggcgat gctgcctctg acatgcagtt
      241 tgtttcgctc gatgcctgac catggatacc ccctcgccag aacactgggc ttgatcgtca
      301 gcacttatct tgtttggatt ctggtgaatc tccacctcgg ctatttcacg gtcatgctct
      361 gctggatcag cctgttcgct ctcgcggcct cgtgttggct cttcgttacc aaggagcatc
      421 ttttccaggt gattcgggaa aagttcagcc tcattcttgt cacagagatt ctgtttctct
      481 caacgttcat catcttcctt gtcatcagag cattcaaccc agagatattc tggggtgaga
      541 agacgatgga cttctccttc tacaacgccg tcgtgcgagg ctcaaccttt cctccctatg
      601 agccctggtt ctccggaaca cttctcaact attactacta tggagatatc ctgatcggtt
      661 atgtcaccaa actggcttca accccaaccg ctttcggatt caacctctcc atggcaacga
      721 tcccagctct tacagtgctc tgcgctttct cactggtcta caacctgacc aagcgcattc
      781 gatggggagt tctcggagcg ctgtttgtcg gctatatcgg caatttcgac ccagtctatc
      841 agctggcaat caaaggttcg cttgaaaact ccttcaacga tcttctcagg ggagagattg
      901 ttcggcagca tggtttgctc gccggtcttt ggctttcggc ggtggctctg gctcagaccg
      961 ttgttctctc cctggctgcg acggtaaggg cgatcctcag tggtgccctc ttcttccaac
     1021 cctcccatgg cagaatgtgg gatagcttct gggcatcttc ccgagccctt ggggaaggga
     1081 tgatcaatga gtatcccgtc tggagctggc tgtttgctga tcttcatgca cacgtcctgg
     1141 tgatgcctgt ttcgctgttg gcactgtcgt tcatctatct ggcttacacc tcaagaaaag
     1201 ccttgttcga aaaccatctt ctgttgggtg gactgttctt cgccatcacg attgggtcgc
     1261 agttggcaac caatatctgg gacttcatct cctacatggg gcttttcgga ttgatcctct
     1321 ttgtacgcgc gttggtgctg cccaagaaga cgctctgttt cctcgaaacg ccgtccagtg
     1381 tcatcgaagg cggagacagt gacgcgtccg tctcctttca ggaaaacatt gtcgcgccct
     1441 tgtttgtccc tgtctgtggt gtaatcggat tcgttgtggc gtggacatat ctctggcctt
     1501 ccatttgtga acttcatcct tactggctcg gatgtatcaa cagccgaccg gtgggcttgg
     1561 ccatcgccct ggccgtggtg gtgctaagca cagccagagg tctcgtggtg gatgaatccg
     1621 cacagttact cagcgctttc tggaaaatcc tgcgcgagtg cgccgtcccc ttgatcgtta
     1681 tcgttggctt gagcggactg ctcttctacc atttccacat caatcttcat accgatgctg
     1741 caacattcaa gttcaacaat gatgggtaca tcaacttctg gaacgccatg cggcatttcg
     1801 gattcttcat cctcctttcg ctcacctgga tcttcataag ctactattcg gcttttcgtg
     1861 gacgcgtttt gaacaaagac atttccctgc cctctccgat actgccattg gttgttggca
     1921 tcgtcggatc ggcacttgtc gcaagacaac ttcgtctctc tgggttttgg cttcccgcac
     1981 acggaatcgt cgtctacgtc atgctcttgc cggcactggt cagcacgatg atcctctggc
     2041 gcaacagccc caccaagttg ttcatatcaa cccttctgtt tgccggctgg acgatcgcca
     2101 cgatatccga gttgatcgtg atcattgacc gcatgaatac cgtcttcaag ttctaccatc
     2161 acacctggat tttcgttgcg gtgggatgtg ccgccgcggt cggagtgatt cgttcggagt
     2221 ggatggggcg cgttttcgaa aaaaccaagg gcaatctgct atggatcgtg cacatcaaga
     2281 tcgttcgcag catcttctgt cttgcctttc tcttcgcact ctttctaacc ctgatctgta
     2341 cctaccgagc cgtacatgga acggtcacac gaaaccagaa gaagagcgac aagccaaccc
     2401 tgaacggtct gaacttcctc aagcacactc cccaggaagc agaactcttc gaggcggttg
     2461 actggctcaa ccgaaatgtt gccggtgtcc aggtcattgc tgaaggattc accaatatgg
     2521 gctatgatga gtccaccatt gcctgcaaat acaccggtct gccgactctc ttgggatggc
     2581 ctcaccatac gaaacagcgc ggacatacgc cacccgaatt gtccgagcgc gatcaagacc
     2641 tgcgtcagtt gtacacaacc gacgacagcg catcgtcaga ggagatatgc gcaaagtata
     2701 acatcaggta catcttcctg gggcacattg agcgtcgttt gtacccagat acggagacaa
     2761 agctgacaaa tcgaactcat cttgagcccg tattcagaag ccagtctggt gcgtatattg
     2821 tgtttcgggt tgttgaacct tcataggtct tacacaaact cgaaagtccc tttgttttgc
     2881 gaagagcaaa tctgaaagga tagtcaacat gggaaatgtc ttaccagaaa ttgtcgtctt
     2941 ggacttgaaa gccgcactga gcggtgagga tgagcgcgga acccgatggg catacgatgc
     3001 tgctaaggca atcgccgctc ttcaaggcat cgtcaaccgc cgcagaccga atctttttct
     3061 ccgctatctc tccaacggtt tgatgagaga acgcagtcag cccaccgatt cctgttcact
     3121 ggacacatat tggctcaact ggctgcggca gagcgacaga atgctggcag aacacacact
     3181 cgttgagacg gacaagatct gtgaagtctt cacacgattt gcagactcca tccacggcat
     3241 tgtgatctgg gatgagtctg ttccttcaac cgcaaacgtt gcagccaccc ttgccggtgc
     3301 aaagaacctg atccctcttc gtggctctac ggctgcaaac tcgttcttcg ctcagtttcg
     3361 cgaactcctg ccaaacatcc ctgttgagca tgatttgagg gacatgttca ccggcacggg
     3421 caagattcct gagactact
//
LOCUS       DAOQUC010000256         3434 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_31459_length_3434_cov_2.906185, whole genome shotgun sequence.
ACCESSION   DAOQUC010000256 DAOQUC010000000
VERSION     DAOQUC010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3434
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31459_length_3434_cov_2.906185"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1004
                     /locus_tag="PKH07_05630"
     CDS             <1..1004
                     /locus_tag="PKH07_05630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34464.1"
                     /translation="PALLEQASGQIYATESAGSQHRLRVCIPPSSQWTTLTNCPSIDG
                     PFKWDVEGSTCAFVQWETRDWKDKTLIVARLPDFETILTLPQSIFSEPRFDNWQEVTD
                     VAVSPDERSIAIPFRHTDAVAPKDRSSYFDLGGKCKLIVIDILSGQETARADRWASDH
                     DVCWLSDSRQVLFSSFDDESLYRTTRAEVRGGTSYGIGYAHDGKFKRGLYVFNIETST
                     TTRFADGYDQSLALATNEILVRNQDGFTLLDSSGTAQERVNARRIGFRGAVVSPSGDY
                     ILATIQRHVPFYAGGRLVLFHKSTPNIRHMLDDDFSCRVDWTTGDKGISNQGVQATP"
     gene            complement(1379..2455)
                     /locus_tag="PKH07_05635"
     CDS             complement(1379..2455)
                     /locus_tag="PKH07_05635"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HNT34465.1"
                     /translation="MIGSDVYFAPVSENEAVESIQEKVVRLYDAAGIGDCIQENDLVG
                     LKIHFGEKGNTTHICPDYVKPVVDLVKLRKGNPFWIDTNTLYNGQRAVTPNHLRVAEE
                     HGFTIERTGAPVVIIGGLTGNDACLLPVKGKYYEEVSVALDLERINSLVILSHATGHM
                     VSGFGGALKNVGMGLSSRRGKLDQHSDVKPFVREDKCRGCTVCLNWCPVQAIAMHDKK
                     AGIDGELCIGCGECLAACRYKAIGFNWSATSQVLQEKMVEYTSGILRSKAGRFGLMNF
                     LINVTKDCDCLGTPNEILAPAIGILASKDPVAIDAASLDLFRKHCGKTLCESSYPHLD
                     SEHQIKHAVELGLGNREYRLVEIA"
     gene            2566..>3434
                     /locus_tag="PKH07_05640"
     CDS             2566..>3434
                     /locus_tag="PKH07_05640"
                     /inference="COORDINATES: protein motif:HMM:NF027716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HNT34466.1"
                     /translation="MSVPTIRVKSTSAPKRALALDALRGLAILGMALSGLVPYGVLPS
                     WMYHAQVPPPNHVFDGSIPGITWVDLVFPFFLFSMGAAFPLALRRKTEDGTPYWKLSL
                     HLLERGVLLLGFGVYIQHIRPYSLNPSPQTETWVTALVGFLILFPVLTRLPKSWNQKT
                     QWAIRLAGWAAVIILMLLVRFPDDKKLSIARNDIIIIVLANMAFFGGLIWLLTRDNWM
                     LRLGVLGAMIGFRLAQKESGWVSVVWSASPIPSLYRFGFLQYLFIVLPGTIAGDQILS
                     WIQSPQQDIESAIP"
BASE COUNT          799 a          968 c          868 g          799 t
ORIGIN      
        1 ctcctgccct tctagagcaa gcgtcaggtc agatttacgc aaccgagtcg gctggttcac
       61 aacacaggtt gcgcgtctgc atccccccga gctcacaatg gacaacgttg acaaactgcc
      121 cgtcgatcga tggtcccttc aagtgggatg tagagggaag cacctgcgcc ttcgtacaat
      181 gggaaaccag agactggaag gacaagaccc tcatcgttgc tcggttgccg gatttcgaga
      241 cgatcctcac actgccacaa tccatattct cggagccacg attcgacaac tggcaagaag
      301 tcaccgacgt ggcagtgtca ccggatgaga ggagcattgc tatcccgttt cgccacactg
      361 acgctgtggc gccaaaagat cgttccagtt acttcgacct cgggggcaag tgcaagctta
      421 tcgtcatcga cattctttcg ggacaggaga cagcaagagc agatcggtgg gcttcagacc
      481 atgatgtgtg ctggctatca gactcccgcc aagtcttgtt ctcatcgttc gatgatgagt
      541 cgctttatcg gaccacaagg gctgaggtcc gcggtggcac aagctacggc attggctacg
      601 cacacgacgg aaaattcaag cgaggtctct atgtcttcaa cattgagacc tcaacaacca
      661 ctcgctttgc tgacggatat gatcagtcgc tcgcattggc aacaaacgag atcctcgtgc
      721 gaaatcaaga tggcttcaca ctgcttgatt catcgggaac tgcacaggaa agagtcaatg
      781 cgaggagaat cggctttcgc ggagccgttg tgtctccatc tggagactat attctggcaa
      841 cgatccagag gcacgtccct ttctacgctg gcggtcgcct tgttctcttt cacaagagca
      901 cacccaacat ccggcatatg ctggatgacg acttttcatg cagagttgac tggacaactg
      961 gagacaaagg aatctcgaat caaggagttc aggcgacgcc gtaagccacg agcctgactc
     1021 cacacgttgt tgatgactct gaacaacggg gtttgtagat aaaggctctt ggcgaacatg
     1081 gctttgaaaa catggattca gcggttcgtg gtgtgctcgt gaggacatat cccgagaaac
     1141 gcctgacggc gtcacgaaaa atcgagtccg agcaaagacg gtggaactcg cagactcgtt
     1201 gccaccctac ctgctggctc agaatccccc catgatcccc tcttatcgta aggggagttt
     1261 tcggaatccc cccttagtaa ggggggcagg ggggattttc ctgttttgga cagcgtcaca
     1321 gatattgccc cgagcgcggt cgaagagcct ccgcacccaa agaaacgtac gcagggtgtt
     1381 acgcgatctc caccagccga tattctcggt ttccaagacc caactctacg gcatgtttga
     1441 tctgatgttc tgaatccaga tgcggatagg acgactcgca cagcgtcttc ccacagtgct
     1501 ttcggaagag atccagagat gccgcgtcaa tggccacagg atctttggaa gccaggattc
     1561 caatcgcagg cgcgagaatc tcgtttggcg ttccaagaca gtcacagtcc ttggtgacat
     1621 tgatcaggaa gttcatcaaa ccaaaacgac cggctttgga gcgcagaatc ccagaggtgt
     1681 actcgaccat cttctcttgc agcacctgac ttgtcgcaga ccagttgaac ccaatggctt
     1741 tgtaccgaca cgccgcgaga cattccccac agccgatgca gagttcaccg tctattccgg
     1801 ccttcttgtc gtgcattgca atggcctgca ccggacacca gttcagacag accgtacatc
     1861 ctcgacactt atcctctcga acgaacggct tgacatccga atgctgatcc aatttccctc
     1921 gtctgctgga gagccccatt ccgacgttct tcagcgctcc accgaatccg gacaccatgt
     1981 gaccggtagc atggctcagg atgaccaatg agttgatccg ttcgagatcc aacgcaacgg
     2041 agacttcctc atagtacttg cctttcaccg gcagaagaca ggcatcgttg cccgtcaagc
     2101 caccgataat cacgacaggt gctccagtgc gctcaatcgt gaagccatgt tcctctgcaa
     2161 ctctgagatg gttcggcgtc acggcacgct gaccgttgta aagcgtgttg gtgtcaatcc
     2221 agaagggatt ccccttccgg agcttcacca ggtccacaac aggtttcaca tagtccggac
     2281 agatgtgtgt cgtgttgccc ttctcgccaa aatgaatttt cagtccaacg agatcattct
     2341 cctgaatgca gtcgccaatt cctgccgcgt catagagacg aacgaccttt tcctggattg
     2401 actcaaccgc ttcattctca gaaaccggcg cgaaatagac gtccgaaccg atcatcaaca
     2461 atacctcctg tatatcagaa caaatgggtt gttctggaaa gaactatggg gcatgataga
     2521 gagaccgttc gaaaatgaca agaaagaata ggctcttgga gaatcatgtc tgtcccaacg
     2581 atcagagtta aatccacttc cgcccccaaa cgtgctttgg cgcttgacgc tctccgtggc
     2641 ttggccattc tcggcatggc actgtccggc cttgttccct atggcgtctt gccctcatgg
     2701 atgtaccacg cacaggttcc acctcccaac catgtgtttg acggttcaat tccaggaatc
     2761 acgtgggtgg acctggtttt cccattcttc ctgttctcga tgggcgcggc gttcccgttg
     2821 gcactgcgtc gaaaaacaga ggatggcacg ccatactgga aactgtctct tcatcttctc
     2881 gaacgcggag tcctgctgct gggtttcggt gtctacatcc aacacatcag accctattca
     2941 ctgaacccaa gtccccagac ggagacttgg gtgaccgcgc ttgtcggatt cctgattctc
     3001 ttccctgttt tgactcggct gcccaaatca tggaaccaga agacgcaatg ggcaatacga
     3061 ctcgccggat gggctgcggt gatcatcttg atgttgctgg ttcgcttccc ggacgacaag
     3121 aagctctcca ttgcccgaaa cgacatcatc atcatcgtgc tggcaaacat ggcgttcttt
     3181 ggcgggctga tctggctgct gacccgagac aactggatgt tgcgactggg tgtactggga
     3241 gcgatgatcg gattcagact tgcacaaaag gaatcagggt gggtttcagt cgtttggagc
     3301 gcgtccccga ttccgtcttt gtacagattc ggctttcttc agtacctgtt catcgtgctc
     3361 ccaggaacca tcgcgggtga ccagattctc tcctggattc aaagcccgca gcaagacatt
     3421 gaatccgcca ttcc
//
LOCUS       DAOQUC010000257         3429 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_31514_length_3429_cov_3.756965, whole genome shotgun sequence.
ACCESSION   DAOQUC010000257 DAOQUC010000000
VERSION     DAOQUC010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3429)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3429)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3429
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31514_length_3429_cov_3.756965"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3429)
                     /locus_tag="PKH07_05645"
     CDS             complement(<1..>3429)
                     /locus_tag="PKH07_05645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34467.1"
                     /translation="QPGSSEKQRVYPFLPASVRLNTTPTEGISGELIYVGKGEPTQLP
                     AKRLQGQIAVMEMSGAWSFVEVYNAGAKAIVLIGSPTDEFKHFDSHLFRMPINVPRFY
                     IPPSSFADSLRSNSYQKARLQSHCFWKETTATNVYALVKGRQQDKPQQAVVIAVQFDA
                     FSPVPEICPGADAAVDVAFALNLIREYAKNPPSRPVLFAFLDAFSHHSKGMREMLAAL
                     SVIPEDRKDFDSEDRKLLKEYLKHWELVRDLESEEDPLESLFLDEYKPLHRYLKDEVS
                     REVVSIESKLFPLRVALHRADSAEAGTLKEKIDFLAARRTEFFAAQRQLLTKTSVADS
                     MRPLAQTLWQRAHERVQGQLKDIEQVLEVTNTRDRMREEIRSLLGLPDSQERPLGFVL
                     GVDLSDAGFSVGPALTCRYSGLNETANSQDFRRWLNTIQKKEFDALWPAELGHAVSFA
                     PLLGLDSPDSYTIGEQANITWPAAAFGLAGMTWTTLEGLRSRLGTPNDQFEALNWDRL
                     NPQIDATLALINRLCHQTDFQQTTKVLPKWTRVRGTIVDQSAGEPVPRLPMKGYVTTL
                     INGSCNADQAGVSWLPSCAGIQRQEFCMTKIDGSFLFDALPGNLAAGYGRFYVQSYLL
                     AQDGHIMRAVDIAKGGKGVDPNVDTASRMASPPKAVVFDCEEMTQMGLFDPRFLWGLP
                     GGTIMDAARAGKPQRLNFTLSGGHMTCQLEPGTKWQAIFRVGTTRNRMSLLNMMDPDD
                     AYDLPERQAMKGFALNQRLPSLPIHIGAKDMSALDEVRLRKYRTAGITSEAIDRLRER
                     TKALLSQADEAIQNDDGGALYNAATGAQANEVRAYQAVRDLADDIVRAAIFLLIALAP
                     FSYAMERLLVASPNIYKQIAGMTLIFCIMTAILWSFHPAFRMSSQPLMIVMAFGIIFM
                     SLLVITVVYAKFETGLEEMRTGRTEGSGASTSRVGVLSSAIQLGIANMRNRMMRTTLT
                     GATVVLITFVLLCFTSTSSYVGQKERRIPISPSYSGVLIHQPSSQPMSGTALTYLKTL
                     AKGRFPVLPRLWWCNPWNPQWKIHIFNPTTHQTVSLTAGIGLNRDEDKATQVRAVLPD
                     WDTFRATGGCYLSEQTASDLGLQPGDSVLVGGISLPLVGVFDSARFDSELSDLDG"
BASE COUNT          796 a          904 c          937 g          792 t
ORIGIN      
        1 ccgtccaggt cagacagttc actgtcaaaa cgagccgagt caaatactcc gacgaggggg
       61 agtgaaatac ctccaacaag aacactgtct cccggctgaa gacccaagtc ggaagcagtt
      121 tgctcagaaa gatagcagcc tcctgtagct cggaacgtat cccaatcggg caggacagcg
      181 cgaacctgag ttgccttgtc ttcatccctg ttcagtccaa tgccagcagt caatgagacc
      241 gtttggtgag tcgttgggtt gaagatgtga atcttccact gtgggttcca cggattgcac
      301 caccagagac gagggaggac tgggaaccgt cccttggcaa gagtcttgag ataggtcaga
      361 gccgttcccg acattggctg actggacggc tgatgaatca gaacgcccga ataactcgga
      421 gagatcggaa tacgccgctc tttctgaccc acgtaactgc tcgtggatgt gaagcacagc
      481 agcacaaaag tgatcagaac aaccgtcgct cccgtgagtg ttgttctcat catccggttg
      541 cgcatgttgg cgattccgag ctggattgca ctggaaagta cgccgacgcg tgaagttgac
      601 gcgccgcttc cttcggttcg cccggttcgc atctcttcaa gaccggtctc gaattttgca
      661 tagacaactg tgatgaccag aagactcatg aagatgatcc caaaagccat gacgatcatc
      721 aacggttgac tgctcattcg gaaggcgggg tgaaaactcc agagaattgc agtcataatg
      781 cagaagatca acgtcatccc ggcgatttgt ttgtagatgt ttgggctggc aaccagcagt
      841 ctctccatcg cataggaaaa tggggcaaga gcgatcagca gaaagatcgc cgcgcgaaca
      901 atatcgtctg ccagatctct gacggcttga taggctctga cttcattggc ttgagcgcct
      961 gtcgccgcgt tgtacaaggc accgccatcg tcgttctgaa tcgcttcatc ggcttgactg
     1021 agaagggctt tggtgcgctc gcgaagtcgg tcaatggctt cacttgtgat ccccgctgtg
     1081 cggtactttc gaaggcgaac ctcatccaga gcactcatgt ccttggcacc gatgtggatt
     1141 ggaagcgacg ggaggcgttg gttgagtgca aaacccttca ttgcctgtcg ctcgggcaaa
     1201 tcataggcgt catccggatc catcatgttc aagagactca ttcgattgcg tgttgtgccg
     1261 actcggaaga tggcctgcca cttggtgcca ggctccaact gacacgtcat gtgtcctcca
     1321 gagagcgtga agttcagacg ttgcggcttt cctgcacgtg ctgcatccat gatggttcca
     1381 cctgggagtc cccagagaaa tcgaggatca aagagcccca tctgcgtcat ttcttcacag
     1441 tcgaacacca cagcctttgg cggacttgcc attcgagagg cggtgtccac gtttgggtca
     1501 acgccctttc cgcccttggc gatgtccacc gcgcgcatga tgtgcccatc ttgtgcgagc
     1561 aggtaagact gaacgtagaa ccggccgtaa ccagcagcga gatttccagg caaggcgtca
     1621 aaaagaaaag aaccatctat cttggtcatg cagaactctt gtctctgaat gcctgcacaa
     1681 cttgggagcc agctgacacc ggcctgatca gcattgcacg aaccattgat gagtgtcgtg
     1741 acataccctt tcatgggaag gcgaggcacc ggctctcctg ccgactgatc cacaatagtc
     1801 ccgcgtacac gtgtccattt tggcaggact ttcgttgtct gctggaagtc agtttggtgg
     1861 caaagcctgt taatcagcgc tagcgtcgca tcaatctgtg ggttcaaacg atcccagttc
     1921 aaggcttcaa attggtcatt gggtgttccc agacgtgaac gcaagccttc gagcgtcgtc
     1981 caggtcattc cggcaagccc aaaagccgcg gcaggccatg tgatattggc ttgttcaccg
     2041 atggtgtagg agtctggtga atccagaccg agaagaggcg cgaaactgac tgcatggccg
     2101 agttcagcag gccagagcgc atcgaattcc ttcttctgaa ttgtattcag ccagcgacgg
     2161 aaatcctgcg agtttgcagt ctcattgagg cccgagtaac gacacgtcaa tgccggtcca
     2221 acggagaagc ctgcgtcgga gagatccacg ccgaggacaa atcccagcgg acgctcctgt
     2281 gagtcaggca ggccgagcag cgatcgaatc tcctctctca ttcgatctcg ggtgttggtg
     2341 acctcgagca cctgttcaat atccttcaac tgaccttgaa ctctctcgtg tgcacgctgc
     2401 cagagggttt gagccagagg gcgcatggag tcagcgacag aagttttggt gagaagttgc
     2461 ctctgtgccg cgaagaattc cgtcctgcgg gctgccagaa agtctatctt ttccttcagt
     2521 gttcccgcct ctgcagaatc tgctcgatgc aaggcaactc gaagagggaa caacttcgat
     2581 tctattgaaa cgacctctcg ggagacctcg tccttgagat agcgatgcag aggtttgtac
     2641 tcgtccagga aaaggctttc cagtggatct tcttcggact ccagatcacg gacgagttcc
     2701 cagtgcttca agtactcctt gagtaatttg cggtcttcgc tgtcaaagtc ctttctgtct
     2761 tcgggtatca ctgaaagggc ggcaagcatt tccctcatgc ctttcgaatg gtgcgaaaaa
     2821 gcatcgagaa aggcaaataa caccggtcga gacggtggat tcttggcata ttcacgaatc
     2881 aagttgagcg caaacgcgac atctactgcg gcatcggctc cgggacaaat ctccggcacc
     2941 ggggagaagg cgtcaaactg cacggcgatg actacagctt gctgtggctt gtcctgttgg
     3001 cggcctttca ccagagcata gacattggtt gctgtcgtct ctttccagaa gcaatgcgac
     3061 tgaagacgcg ccttctggta tgagttggag cggagactat ccgcaaaaga gctcggagga
     3121 atgtagaatc tcggtacatt gatgggcatt cgaaacagat gagagtcgaa gtgtttgaac
     3181 tcatcagtgg gtgagccaat aagtacaatt gcttttgcac cggcgttgta gacttcgaca
     3241 aaactccacg cgcccgacat ctccataact gcgatctgac cctgaagacg tttggcagga
     3301 agttgtgtcg gctccccctt gccaacatag atcagctctc cagaaatgcc ttctgtgggt
     3361 gttgtgttga gtcgcacgct ggctggcaga aaaggatata cgcgctgctt ttccgacgaa
     3421 ccaggctgg
//
LOCUS       DAOQUC010000258         3428 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_31529_length_3428_cov_3.653128, whole genome shotgun sequence.
ACCESSION   DAOQUC010000258 DAOQUC010000000
VERSION     DAOQUC010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3428)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3428)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3428
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31529_length_3428_cov_3.653128"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..837)
                     /locus_tag="PKH07_05650"
     CDS             complement(<1..837)
                     /locus_tag="PKH07_05650"
                     /inference="COORDINATES: protein motif:HMM:NF013479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HNT34468.1"
                     /translation="MPDTPEYGERTEPATPRKREEARKKGQVSRSPEVNYALILLCSI
                     LGLYFMSEYIFERICRTLVHYLQNPLAVDLSRPGAMALMLRLSWETILIFLPFSILIV
                     LAGLTSNLLQVGFMMTGEPLVPKWDRINPISGLSRVFSWRSLAELFKSLAKITAISIV
                     CYITLKAHLPRFFDASYSSTAGILLVFGEVLYILALRCLLVVLLIALVDFAFQRWQFE
                     RSLRMSVQEVKEEMKEMYGDPMIKARVRQIQRQMAQRRMMAKVPEAEVVVTNPTHYAV
                     ALA"
     gene            complement(847..1626)
                     /locus_tag="PKH07_05655"
     CDS             complement(847..1626)
                     /locus_tag="PKH07_05655"
                     /inference="COORDINATES: protein motif:HMM:NF013478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HNT34469.1"
                     /translation="MDLILNQSLNEFGKLLLIMCRVGGLLVFAPVLGSPDVSRQVKAA
                     LILLISLLLAASTTNLATPDITTWLFYTLIPKELTMGAMLGFAAGLIFAAVQYSGSMI
                     GLEIGFRFGTLIDPQADEEVSLVAQFQYLLASLIFVIMGGHRWVLSGLADSFQLVPPG
                     AVRFMPGVEMQLLSMAATIFVTAVQISAPVLLSLLLSSFVLGILSRTVPQLNIINVGF
                     ILKILFGLFFMLVTMDYFGEFMVYLFNSYRRDVLVLWRLFS"
     gene            complement(1664..1933)
                     /gene="fliQ"
                     /locus_tag="PKH07_05660"
     CDS             complement(1664..1933)
                     /gene="fliQ"
                     /locus_tag="PKH07_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HNT34470.1"
                     /translation="MSVLDAVNIGREVVLLALLMSAPMLGVGLAVGLIISILQAVTQI
                     NEMTLTFVPKIILVWITGLLTLPWLIDLLSDYTINLFLRMAAIVR"
     gene            complement(2000..2860)
                     /gene="fliP"
                     /locus_tag="PKH07_05665"
     CDS             complement(2000..2860)
                     /gene="fliP"
                     /locus_tag="PKH07_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012447866.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HNT34471.1"
                     /translation="MRRVHTLVQSRATGTVVRSVLLLLLLTTAVSQVQGQGVPIPRVS
                     VGVDSSSNPKDVAVTLQILFLLTILSLAPAILMLCTSFTRIVIVLSFLRRALGTQSTP
                     SNQILVGLSLFLTFFLMAPVWQEIQDTALGPYLSDEIQPEIRVKEVQGRSVEETITPF
                     QIALERAIVPIRRFMWLQLGTGGAKDVALFLNMANLGQPATEEDVPTRVLLPAFVISE
                     LKKAFIMGFILFVPFLIIDMVTSAILMSMGMMMLPPVMISLPFKLLLFVLVDGWNLVV
                     RAIGLSFIPS"
     gene            complement(2853..>3428)
                     /gene="fliO"
                     /locus_tag="PKH07_05670"
     CDS             complement(2853..>3428)
                     /gene="fliO"
                     /locus_tag="PKH07_05670"
                     /inference="COORDINATES: protein motif:HMM:NF016250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HNT34472.1"
                     /translation="IGSQAKGEPGVDRAIDEVRRQMQQTTDLRDDATDSATTVANKGI
                     PSLFSVIVRLAYALIIVVGFLAAGLYFLRRFFSTRLPGSSRYMQVISRMALSQRSSLH
                     LVRVLNRRLLIGEGPEGVRLIAEIPESDEAIAVKGDEPTSRPMKRAEFRDHLEREAKA
                     VQQETFPDRLQGAVRLCREYIGRFRRGSGDA"
BASE COUNT          899 a          954 c          904 g          671 t
ORIGIN      
        1 agcaagcgca accgcgtagt gcgtcgggtt ggtaaccacc acctcggctt cgggcacttt
       61 tgccatcatg cgacgttgcg ccatctgcct ttgaatttgg cgcacacggg ctttgatcat
      121 cggatcgccg tacatctcct tcatctcttc cttgacttcc tgaacgctca ttcggaggct
      181 tcgttcgaac tgccagcgct gaaacgcaaa atccacaagt gcgatcagga gaaccaccag
      241 caggcaacgc aacgccagaa tgtagagcac ttctccaaag accaacagaa tacccgcggt
      301 actggaatac gaggcatcaa agaaccgagg cagatgcgcc ttcagtgtga tatagcaaac
      361 aatactgatt gccgtgatct ttgccagtga cttgaatagt tccgccagac tccgccagga
      421 aaagactctc gacagacctg agatggggtt gattcgatcc cacttgggaa cgagtggttc
      481 tccggtcatc atgaagccca cctgaagcag atttgacgtc agtcccgcta gaacgatgag
      541 gatggagaag ggcaggaaaa tcagaatcgt ctcccagctc agccgaagca tgagtgccat
      601 cgctcccggg cggctcagat caaccgcaag aggattctgc agatagtgaa ccagcgttcg
      661 acagatgcgt tcgaagatgt actcggacat gaaatacaga cccagaatcg aacacagcag
      721 aatcagcgca tagttgactt caggactgcg tgagacctgt cccttcttgc gcgcttcttc
      781 gcgcttgcgc ggggtcgccg gctctgttcg ctcgccatat tctggggtgt ctggcatccc
      841 gaactcctat gagaacaacc gccagagaac gagtacgtcc cgacggtacg aattgaacaa
      901 gtacaccatg aactctccaa agtagtccat cgtcacaagc atgaagaaaa gtccaaagag
      961 aattttcagt atgaaaccga cgttgataat gttcagctgc ggcacagttc gggagagaat
     1021 tcccaggaca aaacttgaga gaaggagcga cagcagcacc ggagccgaga tctgcaccgc
     1081 tgtcacaaag atcgttgctg ccatgctgag caactgcatc tcaacccctg gcatgaaacg
     1141 aaccgcacct ggcggaacca gttggaaact gtctgccaat ccggacaaga cccatcggtg
     1201 gccacccatg atcacgaaga tcagggaagc cagaaggtac tgaaactgcg ccacaagcga
     1261 gacttcctca tccgcctgcg gatcaatcag agttccaaat cgaaacccga tctccagacc
     1321 gatcatgctt cctgagtatt ggacggcggc gaagatcagt cccgccgcaa aaccaagcat
     1381 ggctcccatg gtcagttcct tcggtatgag ggtgtagaag agccaggtgg taatgtctgg
     1441 tgtcgcgagg tttgtcgttg aggctgccag caggagagaa atcagaagta tcagcgcggc
     1501 cttgacctgc cgcgacacat cggggcttcc cagcaccggc gcaaagacca acagcccacc
     1561 caccctgcac atgatgagga gcagtttgcc gaactcattc aacgactgat tcaggatgag
     1621 gtccatgtct tgtcgcctgg atgaaagatc ctttcgttca tttctagcgt actatggccg
     1681 ccattctcag aaaaaggttg atcgtgtagt ccgagagcag atcgatcagc cagggcaacg
     1741 tcagaagacc ggtgatccag acaaggatga tcttgggaac aaacgtaaga gtcatctcgt
     1801 tgatctgggt cacagcctga agaatgctga tgatcagacc gactgcaagt ccaactccaa
     1861 gcattggagc cgacatcaga agtgccagca agaccacttc ccgtcctatg ttgactgcat
     1921 cgagaacgct catcggatct tctcctcgtt caacgcactt gccgtggcaa gtgggacgaa
     1981 caaacaaacg gcgaccggct caactgggga taaaactgag cccgatcgct cgaacaacaa
     2041 gattccagcc gtctacaagg acgaacagca gaagcttgaa cggcagcgag atcatcacgg
     2101 ggggcagcat catcataccc atggacatga gaattgcgga cgtgaccatg tcaatgatca
     2161 gaaacggcac gaaaaggatg aagcccatga tgaacgcctt cttgagctcg ctgataacaa
     2221 aagccggcaa gagtacccgc gtgggaacgt cttcctcagt tgcgggctgt cccaggtttg
     2281 ccatgttcag aaacaacgct acgtctttcg ctccaccggt gcccagctga agccacataa
     2341 agcgccgaat gggcacgatg gcacgttcca gcgcaatctg gaacggcgtg attgtctcct
     2401 cgacagaacg cccctgaact tccttgactc gaatctccgg ttggatttca tcgctaagat
     2461 agggacccag tgcggtgtcc tggatctcct gccagacggg agccatcagg aaaaaggtaa
     2521 gaaacaacga gagtccaaca agaatctgat tggatggtgt ggactgtgtt cccaacgcgc
     2581 gccgcagaaa ggaaagcaca atgacaattc gggtgaaaga ggtgcagagc atcaggatcg
     2641 ccggtgccag ggagaggata gtcaagagaa atagaatctg gagcgtgacc gccacgtctt
     2701 tcgggttgct tgaagagtcc acaccaacgg aaacgcgggg aatcgggact ccctggcctt
     2761 gcacctgaga gactgcggtg gtcagaagca gcagtaacag aacactcctg accactgttc
     2821 cggttgctct gctctggaca agggtgtgca ctctacgcat caccactccc ccgtcggaaa
     2881 cgcccgatat attcacggca cagtcgaacg gcaccctgga gtcgatcggg aaacgtctcc
     2941 tgttggacgg ctttggcttc ccgctccagg tgatctcgaa actccgctcg cttcatgggc
     3001 ctcgaagtcg gctcgtcacc cttgacagca atggcttcat cagattcggg aatctctgcg
     3061 atgagtcgaa ctccctcggg accttcccca atcaacagcc ttcgattcag cactcgcacg
     3121 agatgcagcg aggatcgctg cgaaagcgcc atacgcgaaa tcacctgcat ataccgtgaa
     3181 gacccgggca aacgagtgct gaaaaaccgt ctcagaaaat acagacctgc tgccagaaaa
     3241 ccgacgacga tgatcagagc gtacgccagg cggacgatta ctgaaaagag agagggaatc
     3301 cccttgttcg ccacggtggt ggcagagtcc gttgcatcat ctctgagatc ggttgtctgc
     3361 tgcatttgac gacgcacttc gtcaatcgct ctatccacgc ctggttcacc cttggcctgc
     3421 gaaccaat
//
LOCUS       DAOQUC010000259         3426 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_31557_length_3426_cov_3.500445, whole genome shotgun sequence.
ACCESSION   DAOQUC010000259 DAOQUC010000000
VERSION     DAOQUC010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3426
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31557_length_3426_cov_3.500445"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>545)
                     /gene="carB"
                     /locus_tag="PKH07_05675"
     CDS             complement(<1..>545)
                     /gene="carB"
                     /locus_tag="PKH07_05675"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008275843.1"
                     /note="four CarB-CarA dimers form the carbamoyl phosphate
                     synthetase holoenzyme that catalyzes the production of
                     carbamoyl phosphate; CarB is responsible for the
                     amidotransferase activity; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carbamoyl phosphate synthase large subunit"
                     /protein_id="HNT34473.1"
                     /translation="SVQEINELSGVDPWFLENLAEIVETERLLQPTSGQKGSEPSLRI
                     DTREGMLAVKQQGFSDRQLALLTGTTESEIRHTRKSLGVEPVYKIVDTCAAEFAAETP
                     YLYSSYEKGWVVVETAQQVTTKKDDQQIPGLGRDSEILPDSGKQKVMILGGGPNRIGQ
                     GIEFDYCCCHAVFALKEMGFE"
     assembly_gap    546..555
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<556..1940)
                     /gene="carB"
                     /locus_tag="PKH07_05680"
     CDS             complement(<556..1940)
                     /gene="carB"
                     /locus_tag="PKH07_05680"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008275843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HNT34474.1"
                     /translation="MPKREDIHSILILGSGPIVIGQACEFDYSGTQGCKALRQEGYRV
                     ILVNSNPATIMTDPDTGDSTYIEPLDVPTVTKIIEKERPDCILPTLGGQTALNLAIDL
                     SKAGVLDKYGIELIGAKREAIEKAEDREQFKLAMQKIGLEVPKSFYARSYREALEMAR
                     EVGFPTIIRPSFTLGGTGAGIAYNREELEYWLKWALDQSPVHEVLIEESVLGWKEYEL
                     EVMRDMADNVVIICSIENFDPMGVHTGDSITVAPAQTLTDREYQRLRDASIAVIREIG
                     VDTGGSNIQFAVNPENGRFIVIEMNPRVSRSSALASKATGFPIAKIAAKLAVGYTLDE
                     LPNDITGETPASFEPSIDYVVVKVPRWNFDKFPGSDPKLGMQMKSVGEAMAIGRSFQE
                     SLQKALRSLEMGRFGFGADGKDKHHIKQFLQGETIQIFREIESLKREIEQELRFPSPQ
                     RIFYIRYALQLG"
     gene            complement(1940..3064)
                     /gene="carA"
                     /locus_tag="PKH07_05685"
     CDS             complement(1940..3064)
                     /gene="carA"
                     /locus_tag="PKH07_05685"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HNT34475.1"
                     /translation="MGNAAYLALEDGTVFAGQSFGALAERCGEVVFNTSMTGYQEILM
                     DPSYKGQIVTMTTPHIGNYGVNWEDVESRRIQVEGFIVRELPRLESNWRSREKLEDFL
                     KSAGLPGIEGIDTRALVRKLRTQGAMKGVLSALDCDPRSLVKKAIESPGLVGVDLVQH
                     VTTENPYNWTEEDVWDPGHTPVEQEREFIVAYDFGIKYNILRCLRNRGFRIRVVPANT
                     SAEDVLAMNPDGVFLSNGPGDPEPLTTPIENIRKIIGRKPIFGICLGHQLLGLAVHGK
                     TFKLKFGHRGGNQPVKDLRSGKVEITSQNHGFCVDVDSIKQGNVALTHVNLNDQTVEG
                     MECPDDRWFSVQYHPEASPGPHDARYLFDRFAQMLREDNA"
     gene            3143..>3426
                     /locus_tag="PKH07_05690"
     CDS             3143..>3426
                     /locus_tag="PKH07_05690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34476.1"
                     /translation="MPESANPLQTSPAKNISGTSEDVNRKRKNRVKIARLQKRKVLLS
                     RHISMQEVRCARSVAHRTHETPTKQGVRLVTRSRLPVIVSFSGFHATGVL"
BASE COUNT          751 a          953 c          890 g          822 t
ORIGIN      
        1 tctcaaaacc catctccttg agtgcaaaga cagcatgaca acagcagtag tcgaactcaa
       61 tgccctgacc gattcgattg ggccctccgc cgagaatcat gactttctgc ttgccactgt
      121 caggcaggat ctctgagtcc ctgcccaagc cggggatttg ctgatcgtct ttcttcgttg
      181 tgacctgttg tgccgtttca acgacaaccc atcccttctc atagctggaa tacagataag
      241 gtgtctcggc ggcaaactcc gcggcgcatg tatccacaat cttgtagacg ggctccacgc
      301 cgagactctt tctcgtgtgg cggatttcgc tctcagtcgt gccggtcaga agagccaact
      361 gtctgtcgga gaatccttgc tgcttgacgg caagcatgcc ttctcgggta tcaatacgga
      421 gtgaaggctc cgaacccttc tggccggaag tcggttgcag cagccgctcc gtctcaacaa
      481 tctcagcgag attctcaaga aaccagggat caacgccgct cagttcgttg atttcctgaa
      541 cggacnnnnn nnnnncccaa ctgaagcgca tagcgaatgt agaagatcct ctgcggactg
      601 gggaatcgaa gttcctgttc gatctcccgc ttgagtgatt ctatttcccg gaagatctga
      661 atggtttctc cctgaaggaa ctgcttgatg tgatgtttgt ctttaccgtc ggcgccaaag
      721 ccaaatcgtc ccatttccag cgaacgaagg gctttctgca gcgactcctg aaaactgcgc
      781 ccgatcgcca tcgcctcacc aacagacttc atctgcattc ccagttttgg gtcgctgcct
      841 gggaacttat cgaagttcca gcggggaact ttgaccacga catagtcaat gctcggttcg
      901 aaggatgctg gagtctcgcc ggtgatgtcg ttggggagtt catcgagtgt gtagccaact
      961 gccagttttg cagcaatctt ggcaatggga aatcctgtcg ctttggatgc cagtgcgctg
     1021 gaacgcgaca ccctcggatt catctcgatg acaataaagc gcccattctc tggattgact
     1081 gcaaactgga tattggagcc accggtatct actccgattt cccggatgac cgcaattgag
     1141 gcatcgcgga gacgttggta ttcgcggtcg gtgagcgtct gcgcaggagc caccgtgatc
     1201 gagtcgccgg tgtgcacgcc catgggatcg aagttctcga tggagcagat gatgaccacg
     1261 ttgtccgcca tgtcgcgcat gacttccagc tcatattcct tccaacccag aacggattcc
     1321 tcgatgagga cttcgtgcac cggactttga tccagtgccc acttgagcca gtattcgagc
     1381 tcttctcggt tgtaggcaat tcccgcaccc gtgccaccca acgtgaaaga cggtcggatg
     1441 atggtcggaa agccgacttc acgcgccatc tccaaagctt cccgataact tctggcatag
     1501 aaacttttgg gcacttcaag gccgattttc tgcatggcca gtttgaactg ctctcggtct
     1561 tccgccttct cgatagcttc gcgtttcgca ccgatgagtt cgatgccgta tttgtccaag
     1621 actcctgctt tggagaggtc aatggccaga ttaagcgccg tttggccgcc aagtgtcgga
     1681 aggatgcagt caggccgttc cttttcgatg attttggtta ctgttggaac atcgagcggt
     1741 tcgatgtagg tgctgtctcc cgtatccgga tcggtcatga tcgttgccgg attagagttg
     1801 accagaatga ctcggtagcc ttcctgacgg agcgccttac agccttgagt gcctgagtag
     1861 tcgaactcgc acgcctgacc gataacgatc ggtccggagc cgagtatcag gatgctgtgg
     1921 atgtcttcac gcttcggcat caggcgttgt cctcccgaag catttgggca aagcggtcaa
     1981 agagatatcg ggcatcatgc ggacctggtg acgcctccgg atggtattga accgagaacc
     2041 agcggtcgtc gggacactcc atcccctcca ctgtctgatc gttcagattg acatgggtta
     2101 atgccacgtt gccttgcttg atagagtcca cgtctacaca gaaaccgtga ttctgtgagg
     2161 tgatctccac tttgccggag cgcaggtctt tgactggctg atttccacct cgatggccga
     2221 acttcagctt gaacgtcttg ccgtgaaccg ccagacccag aagctgatga ccaagacaaa
     2281 tgccaaagat tggctttctg ccaatgatct ttcggatgtt ttcaatcggc gtagtcagcg
     2341 gctcgggatc tcccggtcca ttggacagga agacgccgtc gggattcatc gctagaacat
     2401 cttcggcaga agtgttcgct ggcacaaccc gaatgcgaaa acctcgattt cggaggcatc
     2461 gcagaatgtt gtacttgata ccaaaatcat aggcgacaat gaactcccgt tcttgttcga
     2521 ccggcgtgtg gcctggatcc caaacgtcct cttccgtcca gttgtagggg ttttcagtgg
     2581 tgacgtgctg caccagatcg acgcccacga gaccaggcga ctcgatcgcc ttcttgacca
     2641 gactcctggg gtcacagtca agtgcgctca gaacgccttt catggctccc tgagttcgga
     2701 gctttcgaac aagagcacgt gtgtcaatgc cttcgatgcc gggaaggccc gccgatttga
     2761 ggaaatcctc cagtttttcg cggctgcgcc agttggactc cagcctggga agctcgcgaa
     2821 caatgaaccc ctcgacctgg attcttcggg attcgacgtc ttcccagttg accccataat
     2881 tgccaatgtg cggggtggtc atggtcacga tctgaccctt gtaagaagga tccatcagga
     2941 tttcctgata gccggtcatg gatgtgttaa aaacaacttc tccgcagcgc tcggcaagcg
     3001 cgccgaacga ctgcccagca aagaccgttc catcctcaag cgccaagtac gccgcattgc
     3061 ccatctacac ttctccgatg ggaaccatcc ttcagaagag acagttcctt ccacaaatac
     3121 ataacgtatg gacatagtct gcgtgccaga aagcgcaaac ccgctccaaa cctctccggc
     3181 aaagaatatc tctggaacgt cggaagacgt caacaggaaa cgtaagaaca gggtgaagat
     3241 tgccaggctt cagaaacgca aagtcctgct ttcgagacat atctcaatgc aagaggtgcg
     3301 gtgcgcccga agtgttgcac accgcaccca tgagactcca acgaaacaag gagttcgact
     3361 ggttacacgc tctcgtctgc cagtgattgt ctcattttca ggatttcatg caacaggggt
     3421 gctttg
//
LOCUS       DAOQUC010000260         3417 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_31676_length_3417_cov_4.046401, whole genome shotgun sequence.
ACCESSION   DAOQUC010000260 DAOQUC010000000
VERSION     DAOQUC010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3417
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31676_length_3417_cov_4.046401"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            77..298
                     /locus_tag="PKH07_05695"
     CDS             77..298
                     /locus_tag="PKH07_05695"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HNT34477.1"
                     /translation="MTRYSERGGFYRSRDGMIFGVCRGVAQYFDLSLFWVRIAFVAGF
                     LFSGFFPVGVLYIFMALLMKKEPYWRTIY"
     gene            342..704
                     /gene="pspC"
                     /locus_tag="PKH07_05700"
     CDS             342..704
                     /gene="pspC"
                     /locus_tag="PKH07_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006425395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HNT34478.1"
                     /translation="MEKGLYRARDGAIFGVCKGLADRFDLAVFWVRVAAVALTLFSGL
                     AAGIVAYIVVALVMKPAPAMPFASDDDREFYDSFTHSRSLALNRLKRTMDLLDRRIQR
                     IEDRVTTKDFDWESRMHR"
     gene            786..2333
                     /locus_tag="PKH07_05705"
     CDS             786..2333
                     /locus_tag="PKH07_05705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34479.1"
                     /translation="MWCSSEIIKKVVPVCCLFAVVLTVGLGYYARSPKHLFFPDAMEY
                     AAVARNMARGQGATSQALWVLRLAFTNELPASEVRRPLLLPLWTSLWFRLSAATDCVA
                     IAASGFWWYAAVLLTFLLAWKKGSPEIAAWSVLFLVFDGSFLRYSFCGLSEPLFAFLL
                     IPVFWLLCNSQRPMHFLGVGLLIGLGQWIRTNSLFLLLPALVFVVAKAREKTLLRILC
                     LVVGFGVAVVPIAVRNFSVMGTFALNPLYPYVALNSTPLIPSAHGMERSIEPISPMAY
                     FLSNPAAFFSKWGTHFVNNYQCLAFSIYPLCLGVLIVGCLCLREAQGRKACLFVLLSV
                     VVSLILLSLGEYEDIRFYVPFAPLIFVLAVQVIWEIGNNVGAKPWKTRALLGSLLTVG
                     LIISVNHFSSVDENEGRNWYLERLAARVQTSVPADGVVVSDVPWVTGWRADRISVWLP
                     AKVQELKSIEQDLPVAAVLLTQGALDPREFSPEWGAIHRGESSLNGYMRTTWDDVPGV
                     VLLRKME"
     gene            2425..>3417
                     /locus_tag="PKH07_05710"
     CDS             2425..>3417
                     /locus_tag="PKH07_05710"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNT34480.1"
                     /translation="MGDDARLTTKPMPSKTARPYVLIVDYDPVHSRIALSNLQEKFPD
                     VEFALVRNGQDAVSMMQSRIPDLTITDLQMPGLNGFALLRKIRADEYTREVPVMVLGG
                     VRDMESVRLAVSLGADDFIAKPVDFHVLSKRINQLMAKKKLGLSLRENHRAYKRREVQ
                     LPVEVHLMVVAVGADRFTVLMPGEVVNSASSLFNISRLCRALRVPVVNEPVGCLLGEC
                     RSVKGGYLVDLLLQKLPQGYPESAGRVHDVAGYASQVLGNPRQPVYVGLQCTSLDVSG
                     GGIRIESPWPFPSSAGLRISLRKLLGGLGIRAEDVHVTCIVRHAPKKQHPFHCGL"
BASE COUNT          672 a          880 c          953 g          912 t
ORIGIN      
        1 gagaaggtaa gagcagctga gcagggtgat acacaactgc atttttcagg actaagattc
       61 caaggagaaa acgacaatga caagatacag tgagcggggt ggattctatc gctcgagaga
      121 tgggatgatc ttcggagttt gcagaggtgt tgcccagtac tttgatttgt cgctgttctg
      181 ggttcgcatc gcctttgtgg caggatttct cttttcgggt ttcttcccag tgggagtcct
      241 gtacatcttc atggctctgc tcatgaagaa ggagccttac tggcggacca tctactaagc
      301 gacggattgg gcaattctgt agaacaaaac gggagtggac gatggaaaaa ggactttacc
      361 gagcaaggga tggggcgatt ttcggtgtct gtaaaggact ggcggaccgc tttgaccttg
      421 cagtgttttg ggtgagagtt gcagccgtgg cactgactct tttctcaggt cttgctgcgg
      481 gaatcgttgc gtacatcgtc gttgctctgg tcatgaaacc cgctccggcg atgcccttcg
      541 catcagatga tgatcgggag ttctatgact ccttcacaca ctccagaagt ctcgctttga
      601 atcggttgaa gagaaccatg gatttgctcg atcgtcgcat tcaacgcatc gaagacagag
      661 tcactaccaa ggacttcgat tgggaaagcc gcatgcaccg ctgaccgaaa ccatttgaca
      721 tttctgcaca ttccatgtcc tttattgtgc gagaaggcac tggcaatgaa gcaggagtgt
      781 caccgatgtg gtgttcttcc gaaatcatca agaaagtcgt tccggtctgc tgtctcttcg
      841 ctgttgtgtt gacggtgggg ctgggatact acgcgcgttc accaaagcat ctcttctttc
      901 cagacgcgat ggaatatgca gcggtggctc gaaatatggc ccgaggccag ggagccactt
      961 cccaggcgct ttgggtgctt cgacttgcgt tcaccaatga actgcctgcc tctgaagttc
     1021 gccgaccgtt gctgctgcct ttgtggacaa gtctgtggtt tcgactctcg gcagcgaccg
     1081 actgcgttgc catcgcggca agtggctttt ggtggtatgc cgccgtcttg ctgacctttc
     1141 tgctcgcatg gaagaagggg tcacccgaga tcgcggcgtg gagtgtgctc tttctcgtat
     1201 tcgatggttc cttcttgcgg tattccttct gcggtctgag tgaaccactt tttgccttcc
     1261 ttctgattcc tgtcttctgg ctgctctgca actcgcaacg gcctatgcac ttccttggtg
     1321 ttggactgct catcggtctt gggcaatgga tacgcaccaa cagcctcttt ctcttgctac
     1381 cggcgctcgt ctttgttgtc gcaaaggcaa gggagaaaac gttacttcga atcctctgtc
     1441 tggttgtggg tttcggtgtg gccgttgttc cgattgccgt tcggaacttc agtgtgatgg
     1501 gaacctttgc actcaacccg ctctatccct atgtggcatt gaactcgaca ccgctgattc
     1561 cttcggcaca tggcatggaa agaagtatcg agcccatctc ccccatggcg tacttcctct
     1621 ccaatccggc cgctttcttt tcgaaatggg gaacacattt tgtcaacaat taccagtgtc
     1681 ttgcgttttc gatctatccc ctgtgccttg gagtcttgat tgtggggtgc ctctgtctcc
     1741 gtgaggcaca aggcagaaag gcatgccttt ttgttcttct gtcggttgtc gtgtctctga
     1801 tacttctatc gcttggagag tatgaagaca ttcgattcta tgtgcccttc gctcctctca
     1861 tctttgtgct tgcggtgcaa gttatctggg agatagggaa caatgtggga gccaaacctt
     1921 ggaagactcg ggctcttttg ggctcgctcc tgaccgtcgg tctgatcatc agcgtgaacc
     1981 atttttccag tgtggatgaa aacgaaggac gcaactggta tctggaacgt ctggcagctc
     2041 gggtacagac ttcggtgccc gccgatggag tggttgtcag cgacgtgcca tgggtcaccg
     2101 gttggcgcgc cgatcgcatc tcggtctggc tgccagcaaa ggtacaggag ttgaaatcca
     2161 tcgaacagga tctccccgtc gcagcggttc tgctcaccca gggcgctctg gatccgcgtg
     2221 agttctcgcc cgaatggggt gccatccacc gaggagagag cagtctcaat ggttacatgc
     2281 gcacaacgtg ggacgacgtt cctggtgtcg ttctgttgcg caagatggaa tgagtcaacg
     2341 cccacacaca caattcggtc ttatgtccgc aaccgttttc atttgctttt cggtgtctac
     2401 acggatacac ttcttcggta gatcatgggt gatgatgcgc ggctgacaac caagccaatg
     2461 ccgtccaaga cggctcggcc ttacgtcctg atcgtggact acgatccggt gcattcccgt
     2521 attgcactga gcaatcttca agagaagttc ccagatgtcg agtttgcgct cgtgcgtaac
     2581 gggcaagacg cggtttccat gatgcagtca cgtatcccag acttgacgat tacagatctc
     2641 cagatgcccg gcctgaatgg ttttgcgctg ctgcgcaaga tccgtgctga tgaatacacc
     2701 cgagaggtgc ctgtcatggt acttggcggc gtgagagaca tggaatccgt tcggcttgcc
     2761 gtgagtctcg gagcagatga ctttattgca aagccagtgg atttccatgt actctccaag
     2821 cgaatcaacc agttgatggc gaaaaagaag ttgggcttgt cactgcggga aaaccatcgc
     2881 gcatataagc gccgagaagt ccaattgcct gtggaagtgc acctgatggt tgttgctgtc
     2941 ggtgccgatc gattcacggt cctcatgccg ggcgaggttg tcaacagcgc ttcctctttg
     3001 ttcaatatct cccggttgtg ccgagcactg cgcgttccgg tggtgaacga gccagtcggc
     3061 tgtcttctgg gcgaatgcag atccgtcaag ggtggttatc tggtggatct tctgcttcag
     3121 aagttgccgc agggatatcc tgaatcagcc ggtcgcgttc atgacgttgc cggctatgca
     3181 tcccaggttc tcggcaatcc acgtcaacct gtctatgtcg gactgcaatg cacgtctttg
     3241 gatgtgagcg gcggaggcat tcgcatcgag agtccctggc ctttccccag ctcggcagga
     3301 cttcgaatat cgctcaggaa gcttctcggt ggcctcggaa ttcgagccga ggacgttcat
     3361 gtcacctgca ttgttcgcca tgctcctaag aaacagcacc cgttccactg cggtctg
//
LOCUS       DAOQUC010000261         3416 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_31698_length_3416_cov_2.989289, whole genome shotgun sequence.
ACCESSION   DAOQUC010000261 DAOQUC010000000
VERSION     DAOQUC010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3416
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31698_length_3416_cov_2.989289"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1088)
                     /locus_tag="PKH07_05715"
     CDS             complement(<1..1088)
                     /locus_tag="PKH07_05715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="O-GlcNAc transferase"
                     /protein_id="HNT34481.1"
                     /translation="MSVKRRKRSQELNPSRGFSIVRQSVLTKALLLSFLVIGFYLPAL
                     HAGYIWDDRNVIENPNIVSPKGLFGLWRDPKSISAVESGYWPLTYTSFWIEYRIWGDR
                     PFGYHLTNVVLHLANAMLVWLVLMRLGVPWAWFAALVYALHPMRVESVAWVVERKDLL
                     SGFFYLFSLKAYLSLSQTMRSRHYWFSLAWFICALLSKSVAVTLPAAILAILWWRNGR
                     IERREVLRLIPFFAAGLVLSLLGMVLTRQSGQLMEELGVVERFLVAGRAFWFYAVKTV
                     LPFGLMAVFPKWSVDSTIWWQYLFPIFAALTFAVLWSLRERLGRGAFVSVFYFALTLS
                     PTLGFFNFPLFRYTYVTQRYAYLGSIGL"
     gene            1370..2884
                     /locus_tag="PKH07_05720"
     CDS             1370..2884
                     /locus_tag="PKH07_05720"
                     /inference="COORDINATES: protein motif:HMM:NF012435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HNT34482.1"
                     /translation="MNDSNDRSQWKSSYGFLLAAVGSAVGLGNIWRFSFLCHKNGGGA
                     FLIPYFVALFIVGIPLLVLEFALGHKKQGSAPAALEKVGKEWEWLGWWPVIFVMFGIV
                     LYYNVIIGWCVNYMVMAFRSIWSGFPWAEDSQSFFYNDFLALSDTVWSLGSLRWQILL
                     ATILVWVVNWSICFRHVEKGVEAACKIFMPLLLLLTLTLVFWSLTLPGAMQGVKAYLY
                     PDWTLLSKKQVWQDAFGQIFFSLSLGFGIMIAYASYLPRKVNIIRYALITSLADTLYS
                     FVAGFAVFSILGYMAQEIGATVHGVVTEGPGLAFVVYPKALTLMGNLTGGLLGTIFFL
                     TLCVAGLSSSISIIEAFTAAALDKFKIVRARLVTITCSIGFVGSLVFCSRAGLFWLDI
                     VDHFLNGYGLMTVGLVECLLVGWYYEHGRMRDHLGQSSGKPIGALVASWWDWSIKFIA
                     PGSLFLIIFWSLRGEISHPYGGYPVSAVVIIGGGWVLLTHFAAVFFSHLKWKLR"
     gene            complement(2921..>3416)
                     /locus_tag="PKH07_05725"
     CDS             complement(2921..>3416)
                     /locus_tag="PKH07_05725"
                     /inference="COORDINATES: protein motif:HMM:NF017828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HNT34483.1"
                     /translation="SAKPPDRSMGSRVRVLMTGVPMAHGAEKVLDIIEEHGGLVVSME
                     NCTGLKPIMDDVPCGNGDPLRAIADHYFKLPCSVKTPNNSRLNLIRRLVKEYRPECII
                     DLVWQACLTYDVESHFVSQTAEEMGLPYLRVETDYSPSDTARIAVRVEALFETAGGTP
                     SRYQ"
BASE COUNT          821 a          885 c          868 g          842 t
ORIGIN      
        1 atgagcccta tactcccaag atatgcatag cgctgtgtca catacgtgta gcgaaacagt
       61 ggaaagttga agaaacccag cgtcggggat agcgtcaggg caaaatagaa caccgaaaca
      121 aaagctccac gacccaagcg ctcccgaagt gaccacaaga ccgcaaatgt caaagcggcg
      181 aagatgggaa acagatattg ccaccagatc gttgagtcaa cagaccactt tggaaagact
      241 gccatgagtc cgaaaggcaa gacggtcttc actgcataga accagaaggc tcttccagca
      301 accagaaaac gttccacgac gcccagctct tccatcagct gcccagactg ccgggtcaga
      361 accatcccaa gcagggaaag caccaaccca gcggcaaaga acgggatgag ccgcagcacc
      421 tctcttcgtt caattctgcc gttccgccac caaaggatgg caaggattgc agcaggcaat
      481 gtcacagcca ctgatttgct tagcaacgca cagataaacc atgcgagaga gaaccagtag
      541 tgacggcttc tcatggtctg agatagcgac agataggcct tcagtgagaa caagtagaag
      601 aaaccggaga gaaggtcttt acgttccaca acccatgcaa ctgactcgac acgcattgga
      661 tgaagagcgt agactagtgc agcaaaccac gcccaaggga caccgagtct catcagaaca
      721 agccagacca gcatcgcatt tgccaaatgg agaacaacat ttgtcaagtg atatccaaat
      781 ggtctgtcac cccagatgcg gtattctatc cagaaggaag tataggtcag aggccagtaa
      841 cctgattcaa cagcggagat tgatttggga tctctccaga gcccaaacag ccccttgggg
      901 gatacaatgt ttggattctc aatgacattc cggtcgtccc aaatatatcc tgcgtgaaga
      961 gccggaaggt agaacccaat aaccagaaat gacaacagca gagccttcgt caagaccgat
     1021 tgccgaacga tgctgaagcc tctggaggga tttagttcct ggcttctctt tcgtcgcttc
     1081 acgctcatct cagaatccac cgtcaaccac ctgttccggc ggcgtgccgt catcgggcag
     1141 tcactctccg ttgatctctc tggaatccta gttcccaata atgttgaggt caagcgtgct
     1201 acgaggttct ccccacagac gtatggagga actatccagg acttcgagtg gagcatcaat
     1261 cttgtctcag atcggcaatc caagcttgaa agataggctc atttttggca acattcgacg
     1321 attcaatgcc aatcgagttc ttctgaactc gtctatggag aaccttttga tgaacgattc
     1381 aaacgacaga agccaatgga agagcagtta tggttttctt cttgccgcag ttggttcagc
     1441 cgtcggcttg ggcaacatct ggcggttcag ttttctctgt cacaagaatg ggggcggtgc
     1501 ctttttgatc ccgtattttg tggctctctt catcgttggg attccgcttc tggtgcttga
     1561 gttcgcgttg ggtcacaaaa aacagggttc tgcacccgcc gcgttggaga aggttgggaa
     1621 ggaatgggaa tggctcggat ggtggcctgt catcttcgtc atgttcggga tcgttctcta
     1681 ctacaatgtg atcatcggtt ggtgcgtcaa ctacatggtg atggcgttcc gctcgatctg
     1741 gtctggattt ccatgggcag aagatagtca gtctttcttc tacaatgact tccttgcttt
     1801 gtcggacact gtctggagtc tgggaagctt gcgatggcag atccttcttg ccacgatcct
     1861 tgtctgggtc gtcaattgga gcatctgctt tcggcatgtt gaaaagggag ttgaggcagc
     1921 gtgcaagata ttcatgcctt tgttgttgct cttgaccctc actcttgtct tctggagcct
     1981 gacacttcct ggtgccatgc agggggtcaa ggcatacctt tatccggact ggactcttct
     2041 gtcgaagaag caggtgtggc aggacgcctt cggacagatt tttttctctc tgtctcttgg
     2101 ctttggcatc atgatcgcct acgccagtta ccttccaaga aaggtgaata tcatacgcta
     2161 cgccctgatc accagccttg ccgacactct ctattccttt gtagcgggct tcgccgtctt
     2221 cagcatcctt gggtatatgg ctcaagagat tggcgcaacg gtacacggtg ttgtcaccga
     2281 aggcccgggg cttgcctttg tcgtctatcc caaagcattg acactcatgg gaaacttgac
     2341 aggtggactc ttgggtacca tcttcttttt gacgctctgt gttgcggggc tgtcgtcctc
     2401 gatctctata atcgaggcct tcacagctgc ggcgctggac aaattcaaga ttgtgcgcgc
     2461 ccgtctggtg acgatcacgt gcagcattgg ttttgttgga agccttgtct tctgctcgcg
     2521 agcaggtctt ttctggctcg acattgtgga tcattttcta aacggctacg gcttgatgac
     2581 cgttggcctt gtcgaatgcc ttttggtcgg atggtactac gagcatggaa gaatgagaga
     2641 tcaccttggc cagtcgtctg gaaaacccat aggagctctt gtcgcttcat ggtgggactg
     2701 gtcaatcaaa ttcattgctc ccggctcgct gtttctgatc atcttctgga gccttcgagg
     2761 tgagataagc catccttacg gaggataccc tgtcagtgcc gtcgtcatca tcggtggtgg
     2821 ctgggttctg ctgacacatt tcgcagcagt attcttcagc catctcaaat ggaagttgag
     2881 atagcagaga tattccaagc atttctgctt caggcggtct tcattgatac cgactcggag
     2941 tgcccccagc cgtttcaaag agcgcctcaa cgcgaaccgc aattcgggct gtatcggaag
     3001 gcgagtagtc ggtctccacg cgaagataag gaagtcccat ctcttcggca gtctggctga
     3061 caaaatgaga ttcgacatca taggtcagac aggcctgcca taccagatca atgatgcatt
     3121 ccggacgata ctccttcacc aaccttcgga tcagattcag acgcgaattg ttgggcgtct
     3181 tcacagaaca tggaagtttg aaatagtgat ccgcaatggc tctcagtggg tcaccgttcc
     3241 cacaaggaac atcatccata atcggcttga gtccggtgca attctccata gagacgacaa
     3301 gaccaccatg ttcctcaatg atatcaagca ccttctccgc accatgtgcc atgggaacac
     3361 ccgtcatcaa cacgcgaacg cgtgagccca tgctacggtc aggcggcttt gcagac
//
LOCUS       DAOQUC010000262         3407 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_31811_length_3407_cov_2.977625, whole genome shotgun sequence.
ACCESSION   DAOQUC010000262 DAOQUC010000000
VERSION     DAOQUC010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3407
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31811_length_3407_cov_2.977625"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="PKH07_05730"
     CDS             <1..351
                     /locus_tag="PKH07_05730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34484.1"
                     /translation="RFAPRLGYALLIGYALLINGVGVSASFMDALQPLSENQRYMTLW
                     IPNFTLPVLYWELIDMGRTDLWWVYFLRAPLGLWRLLALLPPLGILFFGLGLMRRVRE
                     IPSATEGVNRQESS"
     gene            complement(317..2911)
                     /locus_tag="PKH07_05735"
     CDS             complement(317..2911)
                     /locus_tag="PKH07_05735"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNT34485.1"
                     /translation="MRRTTQYHRPLFSRQTAIRLCDLFIRCGILLLIAAVPLVLTGKY
                     SALNIKEAIFQFIMMPALLAFFIRCLLVGHIMLPRCPHWSLLGCFLLWTFVSLSYTPA
                     KRTSIRNFVLFACALALLLLFSIYLRKRRFRDTCLLVMAISATLVALGSNCQLNNIFL
                     GILPRTSPDSIDRTWACSTIGHNNGVAVLVMLSSFAVLYFLLTARTRLGRVFSFLVLA
                     FMYYLILVCQTRAVWIACPIGAFLFFLISYREQFKGEYRTRILTGMLTGGIILLALAF
                     LYYAMTSDAEKRSNIPTLRERLSHFSPEILVKNTRTRITAIGTFMIKDRPLVGHGFDS
                     FKYIYPKYQGDYFARFPNSILDPTGRHSDQAHNDFLQVWIELGLPGLVLSVWILLVHL
                     RCAVGAVLEPRAFAEKVLIAASLGATVGLLIVNVASFEFHVVSSAIALLFWIGLFLQL
                     SATGKPYRLPLGAYSSKHALRNGTFVALLILATYGCLYTTRNIMSDHYYVRGKIFRDR
                     GYSLVESGKVDDGIKYVESGAAYFKLAVQLGPYNGLAHYELGQILGKIGELYLTKKRD
                     SKTSERFLYEAANTMTKAGREYQYKGLYYVLGKAHAFLYYMSRRAQDADLAERNLRLA
                     FRIFPRDTNARDQLARFHWYRGEKEKAFQLWREILVYEPDFHRTYHLMDGIRAEESGV
                     TASAEQSYQIVVALAPKEVEAWDRLARLYVREKRFEEAFGLYEDYWKQNQDHLPILRQ
                     YYRICLEAGDDNRLNQVLERAMEQTPILALRMLFESCLERKEYARGEKALEQLESLPQ
                     HARAQKQTRWNRMQLLWQMGRLPEVFQAWHSMRQSEPELRMNEAFYLGQYVLHVPIMT
                     ILAGSRPL"
     gene            complement(2939..>3407)
                     /locus_tag="PKH07_05740"
     CDS             complement(2939..>3407)
                     /locus_tag="PKH07_05740"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNT34486.1"
                     /translation="ILLPAVYLFGGVRLSWTILLLPLVVAIQVLLALGLSFFVAMGNV
                     FFRDLSILMEVILMGWFYMTPVFYPLRYAWDKMQPHPVILNLYLANPMTPIIYAYRRV
                     LLHSVPQAERFPLPLSDGQAAKYLLFSLAFSLIVFILGFLFFKRCQRYVEDYL"
BASE COUNT          850 a          863 c          935 g          759 t
ORIGIN      
        1 cgttttgctc ccagacttgg atacgctctc ctgattggct atgctctgtt gatcaatggg
       61 gtgggtgttt ccgcaagttt catggatgca cttcagccgt tgtccgagaa tcaacggtac
      121 atgactctct ggattcccaa cttcacactt cctgtgcttt attgggaact cattgacatg
      181 gggagaaccg atctctggtg ggtgtacttc ctacgtgctc cgttgggatt gtggagactc
      241 ctggcgttgc tgcccccttt gggcattctg ttctttggat tgggtttgat gcgtcgcgtg
      301 agagaaatcc cctctgctac agagggcgtg aaccggcaag aatcgtcatg atcggaacat
      361 gcagcacata ctgccccaga tagaaggctt cattcatccg gagttcgggt tctgactgcc
      421 tcatggagtg ccacgcctgg aacacttccg gcagtcttcc catctgccag agcaactgca
      481 ttcgattcca tcgcgtctgc ttctgcgcgc gtgcgtgttg gggaagcgac tcaagttgct
      541 ccaaggcttt ttctcctcgg gcatattctt tcctttcgag acaggactca aagagcattc
      601 gcaacgccag aatcggcgtc tgttccattg cgcgctccag tacttggttc aggcggttgt
      661 catcaccggc ttcaaggcag atgcggtagt actgcctgag aattgggagg tgatcttggt
      721 tctgcttcca gtaatcctca tagagtccga acgcttcttc gaaccgcttt tcgcggacgt
      781 agagccgtgc cagacgatcc catgcctcaa cttccttcgg agcgagcgcc acgacaatct
      841 gatatgactg ctcggcagaa gcggtcacgc ctgactcttc cgcccgaatg ccgtccatga
      901 gatgatacgt gcggtggaag tccggttcat acaccagaat ctcacgccac agttgaaatg
      961 ccttttcctt ttcccctcgg taccagtgga agcgtgccaa ttggtctctg gcgttggtgt
     1021 ctcttggaaa tatccggaag gcaagtcgga gatttctctc cgctaagtct gcatcctgag
     1081 cgcgcctgct catgtagtag agaaaggcat gtgccttgcc aagcacgtag taaagtccct
     1141 tgtactggta ctctcgtccc gctttggtca tcgtgtttgc agcttcgtac agaaaacgtt
     1201 ctgaggtttt ggagtcgcgt ttcttggtca agtacagctc cccaatcttg ccaaggatct
     1261 gacccagttc atagtgagcc agaccattgt atgggccaag ttgcaccgcc agtttgaaat
     1321 aagcagcacc cgattcgacg tacttgattc cgtcgtccac tttgccggat tcaacaaggc
     1381 tgtatccgcg gtctcggaat atcttgccgc gaacgtagta gtgatcgctc atgatatttc
     1441 gtgttgtgta gagacatcca taggttgcca ggatcagcag tgcgacgaat gtgccgttgc
     1501 gcagcgcgtg ttttgagctg taagcaccca aaggcagcct atagggtttt cccgttgcgc
     1561 tgagctggag aaaaagaccg atccagaaga gtagcgcgat ggcactgctc accacgtgaa
     1621 actcaaaact ggcaacgttg acaatcaaaa gacccaccgt ggcaccgagt gaggctgcaa
     1681 tcagaacttt ttcggcaaac gctctcggct cgagaactgc accaacagcg catctcagat
     1741 gtaccagcag aatccacaca gacagcacca aacccggcag tcccaactca atccagacct
     1801 gaaggaaatc attgtgagcc tggtcggaat ggcgtcctgt cggatcgagg atagagtttg
     1861 gaaaccgagc gaagtagtcc ccttgatact tgggatagat gtatttgaaa ctgtcaaaac
     1921 cgtgtccgac aagagggcga tctttgatca tgaaagtccc tatcgcggtg atccgtgttc
     1981 gggtgttctt gaccaggatc tcgggtgaaa aatgagacaa ccgttcccga agcgtcggga
     2041 tgttcgatcg cttctcggcg tcactcgtca tggcatagta gagaaacgcg agagccagca
     2101 gaatgatccc gccggtgagc ataccggtca agattcgggt tcggtactct cctttgaact
     2161 gctctcggta ggagatgagg aagaagagaa acgctccaat cggacaggcg atccagaccg
     2221 ctcgcgtctg gcagaccaga atcaggtaat acataaaggc gagtaccaag aagctgaaga
     2281 ctctccctag tcgggttcgg gcggtcagga ggaaatagag gacagcaaag gacgagagca
     2341 tgaccagtac ggcgaccccg ttgttatggc cgatggtcga acatgcccag gtgcgatcaa
     2401 tcgagtccgg actggttcgt ggcaagatgc ccaggaagat gttgttgagc tggcaattgc
     2461 ttcccaacgc cacgagggtt gcggagattg ccatcaccaa caggcaggta tctcgaaatc
     2521 gcctttttcg gagatagatc gagaacagaa gcaaaagggc aagggcgcat gcaaaaagca
     2581 caaaattccg aatggacgtt cgcttggcag gggtgtaact caaactgaca aaagtccaca
     2641 aaaggaaaca ccccagaagt gaccaatgcg ggcaccgtgg aagcatgatg tgtcccacca
     2701 ggagacagcg gatgaagaag gcaagtagcg ccggcatcat gatgaactgg aatatggcct
     2761 ctttgatatt cagagcggag tatttgccag tgagaaccag cggaacggct gctatgagca
     2821 gaaggatgcc gcaacgtatg aacaggtcgc acagtcggat tgctgtttgc ctggagaaca
     2881 gtggcctgtg atattgcgtc gttcttcgca cttgggaacg tgcctccaaa gcggctatct
     2941 aaagatagtc ctcaacataa cgctgacagc gtttgaaaaa cagaaatcca aggataaaca
     3001 caatcagaga gaaagcaagg gagaaaagaa ggtactttgc cgcttgtccg tcgctcaaag
     3061 gcaaggggaa ccgctctgcc tggggcaccg aatgcagaag cactctgcga taggcgtaga
     3121 tgataggcgt catcgggttg gccaggtaga ggttcagaat cacgggatgt ggctgcatct
     3181 tgtcccaggc atatctcagt ggatagaaga cgggggtcat gtagaaccat cccataagta
     3241 tcacttccat cagaatggag agatcgcgga agaagacatt gcccatggca acaaagaacg
     3301 aaagccccaa ggccagaaga acctgaatcg ccacaaccag gggaagcagc agaatcgtcc
     3361 agctcaaccg cacgccaccg aacagataga cggcgggcaa aaggatg
//
LOCUS       DAOQUC010000263         3394 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_31980_length_3394_cov_3.258461, whole genome shotgun sequence.
ACCESSION   DAOQUC010000263 DAOQUC010000000
VERSION     DAOQUC010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3394
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31980_length_3394_cov_3.258461"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1405
                     /locus_tag="PKH07_05745"
     CDS             <1..1405
                     /locus_tag="PKH07_05745"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HNT34487.1"
                     /translation="NTPTAQPEYRSVWADTWNTSILSPSEADDLVQTCRDNNINTIMV
                     EVRKIGDAYYSSNIEPRATNITGGSSYDPLGYLLSIAHDTSGGKKYVHVHAWFVMQRI
                     CKTETLDPQHVLIQHPEYIMSNSTGGTFNSSTSSSYLDPGHPGAVDHNIAVILDCMSN
                     YDIDGINLDYIRYPEGGLSWGYNPVSVSRFNAFYGKSGSPATADPDWADWRRECISQQ
                     VKKLYVKSWQMNSDVMLSACSINWGTNYADYTNSDAYKAVYQDWVGWLQSGIIDYNAL
                     MNYTTTTSRFNGWCNLSLASDDRRGSIIGCGNYLQTTIQNGLNQLLYARGQGAAGLNI
                     YDWGSEVQATSPAQTRATFYSQLKSQLYPSWVDPPDSPWKSSPTVGIFEGTLTDLGVP
                     VDHGTVELDGQPSTRVYTDGSGWYAIMDVAPGSYQLRFKKTGQTDLLLPATIVAGGDI
                     VTVNADFPTAVAQWMIY"
     gene            1508..2293
                     /gene="pyrF"
                     /locus_tag="PKH07_05750"
     CDS             1508..2293
                     /gene="pyrF"
                     /locus_tag="PKH07_05750"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR02127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HNT34488.1"
                     /translation="MNLTERILASQTKRKSLVCVGLDVEPSRHGAGTSLQEIERFLVE
                     AIRLTRDQVAGYKANLAFFLALGTEGILMLKKLRDEIPSDSIYVLDGKWGDIGNTSEK
                     YAEFGFKYLGADVLTAHPYMGRDGIEPFIAEESRGVFLLCRTSNPNGAEIQAYPTPCS
                     PLFMHVAQRAQTWNDRNNIGLVVGATFPQEMKRIRQECPSSIFLVPGVGAQGGRVEDV
                     LESAGRSVLIAASRSLLYPDKDAARSERVAERAAQMNQEIGWS"
     gene            2346..3143
                     /locus_tag="PKH07_05755"
     CDS             2346..3143
                     /locus_tag="PKH07_05755"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNT34489.1"
                     /translation="MSQCQSAQVVPVRTKILVVWILLILSSGLFLFLIPRHTYRHIDT
                     DTDPITATGFRAIESGDLGLAESSLLESVRRNPNQDRPYLGLAMVRRLKGDLEQASRD
                     IEKAILKNPNQPEYHVARASILRDRGQYDQALASCQRAIEADSQHVGAFQEAGYIHYI
                     QGNHREARRLLYRALALKPDLVNAERLLGSSYLAENKPQQAIPSWRRLVEREPANAEN
                     HFYLGVCLLEAGEREEGREELLRSAELDAAVYASRVQDRLTYYGLEN"
     gene            complement(3150..>3394)
                     /locus_tag="PKH07_05760"
     CDS             complement(3150..>3394)
                     /locus_tag="PKH07_05760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34490.1"
                     /translation="VLAEVFPLMRCERFAFDLELIALAHQRGYRMGELPVQLRFRRKM
                     RFGRIRLWDFFLTALDTLGILVRVRLLGQKRSSRKT"
BASE COUNT          783 a          899 c          923 g          789 t
ORIGIN      
        1 caatactccg acggcacaac cagagtacag gagcgtctgg gcggacacct ggaacaccag
       61 catcctctct ccatccgaag cagacgattt ggttcagacg tgtcgggaca acaacatcaa
      121 caccatcatg gttgaagttc gcaagatagg cgatgcctac tacagttcca atatcgaacc
      181 ccgagccacc aatatcacgg gcggctccag ctatgatccg ctgggttatc ttctcagcat
      241 tgcgcatgac acttcgggtg gcaaaaagta cgttcatgtt catgcctggt ttgtcatgca
      301 gcgcatctgc aagacggaga ccttggatcc ccaacacgtt ctgatccagc atcctgaata
      361 catcatgagc aactcgactg gaggtacttt caacagcagc accagctcca gttatctgga
      421 tcccggtcac cccggtgcgg tcgatcacaa cattgctgtc atcctggact gtatgagcaa
      481 ctatgacatt gatggcatca acctggatta cattcgttac cccgaaggtg gcctctcctg
      541 gggttacaat ccggtgagtg tcagccgttt caatgccttt tacggcaaga gtggttcgcc
      601 cgcgacagct gatcctgact gggcggattg gcgacgtgaa tgcatcagcc agcaggttaa
      661 gaaactctac gtcaagtcct ggcagatgaa ttcggatgtc atgctcagtg cctgttccat
      721 caactgggga accaactatg ccgattacac caactcggat gcctataagg ctgtctacca
      781 ggactgggtc ggttggcttc agtcggggat cattgactac aacgctctga tgaactacac
      841 aaccacaacc tcccgcttca acggctggtg caatctgtct ctcgccagtg acgatcggag
      901 agggagcatc atcggttgcg gcaactatct ccagaccacg attcagaacg gtctgaacca
      961 gctgctgtat gcgcgtggtc agggtgctgc cggcctgaac atttacgact ggggcagtga
     1021 agtgcaagca acgagtcccg cccagactcg tgccacattc tacagtcagt tgaagtccca
     1081 actgtatccg tcttgggtag atccgcctga ttcgccctgg aagtccagtc ccacggtcgg
     1141 tatctttgaa ggaactctta ccgaccttgg agtgcctgtt gaccacggga cggttgaact
     1201 tgacggacag ccttcaactc gcgtctacac cgatggcagc ggatggtacg cgattatgga
     1261 tgtcgcgccc ggttcctatc agttgcgatt caagaaaacc ggtcagaccg atcttcttct
     1321 tccggcgacc attgttgccg gtggcgacat tgtcactgtc aacgcagatt tcccgactgc
     1381 tgtggcacag tggatgattt actagcgatg agaacgtgag acggattctc tttcgtattg
     1441 ttgctctaac gaaacttcgg aagtggtctg cccgtcagtg gtgtaccaga aacagagagg
     1501 acgtgtgatg aatcttacag agcggatcct ggcgtcacag accaagcgca agagtctggt
     1561 ctgcgttgga ctggacgttg agccttcccg tcatggcgct gggacgagtc ttcaggagat
     1621 cgagcggttt cttgtcgagg ccatccgact gactcgtgac caggttgccg gctacaaggc
     1681 aaatctcgcc tttttcctgg ctcttggaac cgagggaatt ctcatgctca aaaagctcag
     1741 agatgagatt ccatccgatt ccatctacgt tctcgacggc aaatggggag acatcggcaa
     1801 cacgtccgag aagtatgctg agtttggctt caagtatctt ggtgcagacg tcttgacggc
     1861 gcatccttac atgggacgag acggcatcga accatttatt gccgaagaga gtcgaggtgt
     1921 ttttctgctc tgtcgcacat cgaatcccaa cggtgcagaa attcaggcct atccgactcc
     1981 ctgttctcca ttgttcatgc atgttgctca gagggctcag acttggaatg acagaaacaa
     2041 cattggtctg gtcgttggcg ctacctttcc gcaggagatg aaacgcatca gacaggaatg
     2101 ccccagttcg attttcctgg ttcccggcgt tggagcgcag gggggacgtg ttgaggacgt
     2161 tcttgaatca gctggcaggt cggtcctgat tgcagcgtct cgttcattgt tatatcctga
     2221 caaagatgct gccagatctg agcgtgttgc agaacgagcc gcgcagatga accaggaaat
     2281 cggctggtcg tagtcacaga agaatctggt ggcgaatccg tggagaggtg gatcttttca
     2341 gcgagatgtc ccagtgccaa tcagctcagg ttgtacccgt tagaacaaag atactcgtcg
     2401 tatggattct cctgattctt tcgtctggac tgtttctgtt cctgatacct cggcacactt
     2461 acaggcatat tgacaccgac acggatccga ttacggccac gggatttcgg gccattgaaa
     2521 gtggagacct gggactcgcc gagagcagcc ttcttgagtc agttcgacgc aatcccaatc
     2581 aggataggcc ttatcttggg ttggcgatgg ttcgacggct caagggggat ctggaacagg
     2641 caagccgcga cattgagaag gcaatactga agaatccgaa tcagccggag tatcatgtcg
     2701 cacgtgcctc gatcctgcgc gaccgtgggc agtacgatca ggcgctggcg agttgtcagc
     2761 gcgccatcga ggcagattcg cagcacgttg gagcttttca ggaagccgga tatattcact
     2821 atattcaggg caaccatcgc gaagcgcgga ggcttcttta tcgtgccttg gctttgaagc
     2881 ctgacctggt gaatgccgag cgacttctcg gctcatccta ccttgcggag aacaaaccac
     2941 aacaggcgat tccttcctgg cgcagacttg tcgaacgcga gccggcaaac gctgagaatc
     3001 acttctacct gggtgtttgt ctcttggaag cgggggaaag ggaagaggga agagaggagc
     3061 tgcttcgatc tgcagagttg gatgctgcgg tctatgcctc cagagttcag gacagactca
     3121 cgtattacgg acttgagaat tgaagcctct caggtttttc ggctgcttcg cttttgccca
     3181 agaagacgga cgcgtaccag tatccccaga gtatccagtg cagtgagaaa aaagtcccac
     3241 aggcggattc ttccaaaacg catcttgcgg cggaatcgaa gttggaccgg gagttccccc
     3301 atgcgatacc ctcgctgatg tgccagagcg atcaactcca gatcaaatgc gaatcgctca
     3361 cagcgcatca atgggaatac ctccgccagc accc
//
LOCUS       DAOQUC010000264         3368 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_32315_length_3368_cov_3.194386, whole genome shotgun sequence.
ACCESSION   DAOQUC010000264 DAOQUC010000000
VERSION     DAOQUC010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3368)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3368)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3368
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32315_length_3368_cov_3.194386"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            15..>1969
                     /locus_tag="PKH07_05765"
     CDS             15..>1969
                     /locus_tag="PKH07_05765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34491.1"
                     /translation="MFLYRQDGELAAYRPLLPSGLPSVATEFLDADVVAGIEYRYRVE
                     VREAGEVSTGESEAVVPGARDDSLVLARPMQWLADVPRGTSATLTVTVDGVFNPFQKT
                     RVSIPEELMPDLTGLSAEVGQPLVSIPGTFQVTVHAKHQIRPGTYPIPVRVQSGKTVQ
                     IVWVFVRVSVPFGSETGFAPPYPATSRKVGIHTDSQLREETNNLLVMKGELGDILGNA
                     RGTGVVALVDLGNGQIIEQADRVSNDSYYSLSIPLPSLERDTVVRTRVTWMGSSTSPG
                     GTSPILSLPVGGYSMAAEGGGLQQSRDEPEKAFFVMGVPPSGPSQEDVEQIVEHAKTT
                     VLAEFSREERVTLESKTEIESALSGAQDAQTFLFYAVGDMSQGTVQLSGGETITPQEL
                     SSMVNSLPDTVNSVVIVEGPRSGAFAASGAGYRSGTTVLTSCSARESTNKFSPWYRSF
                     SGAFFDACRVPLSVADSFSVAGLSFLDFFFGQTQTPLLISGKLSNTKLYGRFVPATLP
                     DLVSPEILGFRAERIEGEAFALDALAADNRDSAENLIVTADVLAMDGSVREGLTFAAA
                     DTGNLTHRAFLQQPGIPGFGVILSVRDSSGNMSIPFSTWVATSSSTLAFDINGDGIVD
                     AEDLIQCIAWSVDRTLPSDWQFQLASA"
     assembly_gap    1970..1979
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2135..2998)
                     /locus_tag="PKH07_05770"
     CDS             complement(2135..2998)
                     /locus_tag="PKH07_05770"
                     /inference="COORDINATES: protein motif:HMM:NF033545.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HNT34492.1"
                     /translation="MEVKALACSIPGEHGLPFSRFSHADIAREAMERGITASISGATV
                     WRWLSADAIKPWSHRSWIFPRDPGFAEKAACVLDLYQGFWEGNPLGPNDYVISADEKT
                     SIQARSRIAHDAPPAPKKIRKVEFEYQRKGYLAYMAAWDVHRAKIFGQCEQKTGIDAF
                     HSLVDLVMTREPYCSADHVFWITDNGPSHRGANAVQRLQQWYPNAIQVFTPVHASWLN
                     QIEIYFSIVQRKVLTPNNFADLNTLQDHLLRFQTFYEQIAKPFQWKFTQEDLKRMLDK
                     ISSHNMLPRAA"
     gene            complement(3006..3281)
                     /locus_tag="PKH07_05775"
     CDS             complement(3006..3281)
                     /locus_tag="PKH07_05775"
                     /inference="COORDINATES: protein motif:HMM:NF024940.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNT34493.1"
                     /translation="MPRNSPFEIVLAPDEQQYLQKIASQYTSPYCDVVRAKVVLLAAN
                     GLENKEIGQRLDLPRQIVSKWRKRFFQERLEGLQDQPRRGRPRVFPP"
BASE COUNT          733 a          867 c          969 g          789 t
ORIGIN      
        1 agtcggatgc caaggtgttc ctgtacagac aggacggaga acttgccgcg tatcgcccgc
       61 ttctgccttc cgggttgccc tcagtggcga cagagttctt ggatgcggac gttgttgcag
      121 gtatcgaata ccgatatcgg gttgaagttc gagaagcagg agaagtttcg acgggcgaat
      181 ccgaagcggt tgttcccggc gcaagagatg actccctcgt tttggcgcgt ccgatgcagt
      241 ggctggcaga tgttcctcgc gggacaagtg ccacattgac cgtgacagtt gacggcgttt
      301 tcaacccgtt ccagaaaaca agggtgtcaa ttccagaaga gctgatgcct gatctcacgg
      361 gtctcagtgc cgaagtgggt cagccccttg taagcattcc agggacattc caagtcacag
      421 ttcacgcgaa acaccagata aggcctggca cttatccgat tcccgtccgc gttcaaagtg
      481 ggaagacagt tcagatcgtt tgggtctttg tgcgtgtgtc cgtgcctttt ggctcagaga
      541 caggtttcgc tcccccctat ccggcaacgt cgaggaaagt gggcattcat accgactcac
      601 aacttcgaga agagacgaac aacctgctgg tgatgaaggg ggaactcggt gacattcttg
      661 gcaatgctcg gggaaccgga gtggttgctc ttgttgattt gggaaacggt cagatcattg
      721 aacaggcgga cagggtttcc aacgacagtt actattcact cagcattccg ctgccctcgc
      781 tggagcgtga taccgtcgtt cgaacgcgag tgacttggat gggttcgagc accagtccgg
      841 gggggacaag tccaattctg agccttcccg tgggtgggta ctcgatggcg gcagaggggg
      901 gcggtctaca acagtcgcgg gatgagccgg agaaggcgtt ctttgtcatg ggtgtgcctc
      961 cttcggggcc atcgcaggag gatgtggagc aaatcgtaga acacgcgaag acgacagtcc
     1021 ttgcggagtt cagccgcgag gagcgcgtca cgcttgagag caagaccgag atcgagtcgg
     1081 cgctttcagg tgctcaggat gcgcagacat tcctcttcta cgcagtgggc gacatgtcgc
     1141 agggaaccgt tcaattgtct gggggtgaaa caataactcc ccaagagctc agttcgatgg
     1201 tcaactcatt gcccgatacg gtcaattcgg tcgtcatcgt cgaggggccc cgaagcggag
     1261 cttttgctgc gtcgggtgcg ggatatcgtt ctggaaccac cgttttgact tcctgttctg
     1321 cgcgggagag caccaacaag ttcagtccgt ggtaccggtc attttcgggt gcttttttcg
     1381 atgcatgtcg ggtacctttg agcgttgctg acagtttcag tgttgccggg ctttcgtttc
     1441 tggacttctt ctttgggcaa acccagacgc cccttctcat cagcggaaaa ctcagcaaca
     1501 cgaaactcta tggcaggttt gttcccgcca cgctgccgga cttggtttcc ccggaaatcc
     1561 ttggattccg ggcggagcgc attgaggggg aagcctttgc cttggatgct ctggcggcag
     1621 ataacaggga ctctgcagag aacctcattg ttactgcaga tgtccttgcg atggacggct
     1681 cagtccggga aggcctgaca ttcgcggcag ccgatacagg aaacctgact cacagagcct
     1741 ttctgcagca gccggggatt ccgggtttcg gagtcattct ctctgttcgt gactcctcgg
     1801 gaaacatgtc aatacccttt agcacctggg tcgctacatc atcgagcacg cttgccttcg
     1861 atatcaacgg cgacggtatt gtggatgccg aagatctcat ccagtgcatt gcatggtcgg
     1921 tggacaggac attgccaagt gattggcaat ttcaactcgc atccgcttgn nnnnnnnnnt
     1981 cgcgcctgca agcgtgtggg ctatgcccac tccctcgaat gctcgcttgt caaaagacag
     2041 gcctcttccg aacttctgtg ccacaagaag caggcctgcg ccaacgcctt tctaagtgct
     2101 gtagttcata agttcggtga cgtatcattg gcgttcaggc tgcacgaggc agcatattat
     2161 gggacgatat tttgtcaagc atccgtttaa gatcctcctg tgtgaatttc cactggaaag
     2221 gtttcgcgat ttgttcatag aaagtctgaa agcgcaagag atgatcctga agtgtgttca
     2281 gatcggcgaa gttgttgggc gtaagaacct tgcgctgaac gatggaaaag tagatttcga
     2341 tttggttgag ccaactggcg tgaacgggag taaagacctg aatagcattt gggtaccact
     2401 gctgaagcct ctggacagca ttggcgcctc ggtgcgaagg gccattgtca gtgatccaga
     2461 agacgtggtc ggcagaacaa tacggttctc tggtcattac aagatcgaca aggctatgaa
     2521 aggcgtcgat gccggttttc tgttcacact gtccgaatat cttggcgcga tgcacgtccc
     2581 aagcggccat ataggccaag tatcccttgc gttgatactc aaactcaacc ttgcggatct
     2641 tcttgggagc gggcggggca tcgtgcgcga tgcgagaacg cgcctggatg ctggtcttct
     2701 catccgcgct aatgacgtaa tcattcgggc caagagggtt gccttcccag aaaccctggt
     2761 acaagtccaa gacgcaagca gctttctccg caaaccccgg gtctcgcgga aatatccagc
     2821 tgcggtgcga ccaaggcttg atagcgtccg ccgagagcca tctccacaca gtagcgccgc
     2881 ttatggaggc tgtaatgcct ctctccatcg cctcgcgggc tatatcggca tgactgaagc
     2941 gggagaaagg aaggccgtgc tcaccaggga tagagcaggc caaagccttg acctccacaa
     3001 cgacctcagg ggggaaaaac gcggggccgt ccgcgtctgg gctggtcctg aagcccttcc
     3061 aggcgctctt gaaagaagcg ttttcgccac tttgagacga tctgccttgg gagatcgagt
     3121 cgttggccga tctctttgtt ctccagtcca tttgcagcca ggagaacgac cttggcgcgc
     3181 acaacatcac aatacggtga cgtatattgg ctggcaatct tctggagata ttgttgctca
     3241 tcaggggcaa gaacaatctc gaatgggcta tttctgggca tatcacacct cctacggaag
     3301 aggatattgc cacatttgct ccagtaatgc caccaaaata cgtcatcata tatgagaaca
     3361 gatgtact
//
LOCUS       DAOQUC010000265         3367 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_32329_length_3367_cov_3.277476, whole genome shotgun sequence.
ACCESSION   DAOQUC010000265 DAOQUC010000000
VERSION     DAOQUC010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3367
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32329_length_3367_cov_3.277476"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /locus_tag="PKH07_05780"
     CDS             <1..349
                     /locus_tag="PKH07_05780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34494.1"
                     /translation="WMMLFLNTDRDPKTGWEGYNFVVNRLAATEKVLHLERSSGGWNW
                     QPAGQISYAVQGCEMELAIPRSLLNLEPSSKVDVEFKWADNIQQDDNIMEFVVSGDCA
                     PSGRFNYRYSEIP"
     gene            689..1024
                     /locus_tag="PKH07_05785"
     CDS             689..1024
                     /locus_tag="PKH07_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HNT34495.1"
                     /translation="MALITEEQLKKMTEIVAREVDPEAIVLFGSHAVGKAHEDSDVDL
                     LIIESASFSPGRGRRKEMARLWQALMGFPVAKDILVYSREEVERWRHSRNHIIARALR
                     EGRVLYERP"
     gene            1011..1394
                     /locus_tag="PKH07_05790"
     CDS             1011..1394
                     /locus_tag="PKH07_05790"
                     /inference="COORDINATES: protein motif:HMM:NF017020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HNT34496.1"
                     /translation="MKDREEALRLLFAAEKDIAALRGMMDLAVFAEEIFGFHAQQAVE
                     KSLKALLALLGRQYPRTHDLSLLMSQLSSSGEIVEEFIDLLELNSYAVQFRYEAFDES
                     DEGLDREQVVDRVTALAQSVRNRID"
     gene            1398..2564
                     /locus_tag="PKH07_05795"
     CDS             1398..2564
                     /locus_tag="PKH07_05795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34497.1"
                     /translation="MKAVVRSPLSPFSFLTEMVLLCLCAAGQGREISTPGAVHGANAD
                     GTVGSSTEVCITGVPGWTMEKDSSVHAAHSEGDKIMKVDGVPELGWGKGKNCTFIGAL
                     EAALAVTDHPAKYSDLMGWSGMAFRVRWYVGNDNSGACPSAAVGEFEDETAAVQKYSG
                     WSAFADFDPTPSALLRFKDKITASIDKGMPVLLYDPGWDVAVAYGYEKGGDRLFLRSY
                     TDNFSQIDLASSPALIYINEKYAEPPSRRDAFIDALNLAVSNWHREAMVREKGQYLYG
                     ELGFDKWMQDYTNFKSLSEEAQKTTAHASRFCYMTLLDCRAAAATFLDEHSDAVNEEA
                     RICLKRAADICRRQLPDEIAMGTPNGVPEHLLKAKEEFSEVIAEIGKALAAERH"
     gene            complement(2660..>3367)
                     /locus_tag="PKH07_05800"
     CDS             complement(2660..>3367)
                     /locus_tag="PKH07_05800"
                     /inference="COORDINATES: protein motif:HMM:NF013703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HNT34498.1"
                     /translation="HHLLVFRKWRLREYRDTARRILRFAVPSMLSMMLMPISAGVITR
                     LLGRFGNEPVAAVGAAGRIEMFAFMIPMALGISLTPFVSQNFGAKRLDRIQEALQVTT
                     RFAMLYGIGVAVVFFCGAPWLAAVFSKDPGVVNVLVAYVRIISFGYGMMEVHRYCGFF
                     FTGLHHPAFSTALNALRVLVFLVPLTVSGAYFFGVQGVFFGRLLTDLLAGTIGLFWVH
                     RQLRAIRASAMRTVEQA"
BASE COUNT          830 a          846 c          946 g          745 t
ORIGIN      
        1 atggatgatg ctcttcctga acacggatcg cgatcccaag actggttggg aaggttacaa
       61 cttcgttgtg aaccgtcttg ctgcgactga gaaggtgctc catcttgagc gaagttcagg
      121 tggttggaac tggcaaccgg caggccagat ttcctatgct gttcaaggct gtgagatgga
      181 actcgcaatt ccccggagtc ttctcaatct ggagccatcc agcaaggtgg acgttgagtt
      241 caaatgggca gacaacatcc agcaggacga caacatcatg gagttcgtcg tcagcggtga
      301 ctgtgctccc tcgggtcggt tcaactaccg gtattcagaa ataccttagg cgggctctac
      361 gcggagcagg ccaacgctta caccgtgtgg gctatcccct cgcttatggg tgtttgctca
      421 acaaacgagg tgtgttcctt tgactcagtg ccagaacgaa cgacgcaaag atcacttcac
      481 attactgcaa tgttctgagg tgccaaccaa ctcccacccc atattctgtt gacccagtgt
      541 ccgttcctct gtagattgga aatgtaggcg agagatcagt tggtgagatc tcgagcagtc
      601 cagcacttcc aggtgcgggg tctgttcact ctcctcctgg tacttgccta atgcagattg
      661 cgtccttagg acttggtgtc aacggaaaat ggctctgatt acagaagaac agttgaagaa
      721 gatgaccgaa atagtcgctc gggaagtgga tccggaagcc atagtgcttt ttggatcaca
      781 cgccgtcggc aaggcgcatg aagactctga cgttgatctt ctgatcattg agtcggcgtc
      841 ctttagtccg ggtcggggaa gacgcaaaga gatggctcgg ctgtggcaag ctttgatggg
      901 ttttcccgta gccaaggata tattggtcta cagccgcgaa gaagtcgaac gttggcgtca
      961 ctctcgaaat cacatcatcg cgcgagctct acgagaagga cgtgttctct atgaaagacc
     1021 gtgaagaagc acttagactt ctgtttgcgg ctgaaaagga cattgccgca ctgcgcggta
     1081 tgatggatct ggcagttttt gcagaagaga tttttggctt tcatgcccag caagcagttg
     1141 agaaatccct caaagcgttg ttggctctcc ttggccgaca gtatccaagg acgcatgacc
     1201 tctccttgct tatgtcccag ctgagctcat ccggagagat agtcgaggaa ttcatagatc
     1261 ttctggaact caactcttac gctgtccagt tccggtatga agcctttgat gagtccgacg
     1321 agggactcga tcgggaacaa gttgtcgatc gcgtgacagc acttgcgcag agcgttcgaa
     1381 acaggattga ttgacagatg aaggcagtgg ttcgatcacc cctttcccct ttctcatttc
     1441 tgaccgagat ggtactgttg tgtctatgtg ctgccggaca gggtcgggag atctcgactc
     1501 caggtgcggt gcatggagcg aacgccgatg gaaccgtcgg tagttcaact gaggtttgta
     1561 tcaccggggt tccgggctgg acgatggaaa aggacagttc ggttcatgct gcgcactcag
     1621 aaggagacaa gatcatgaag gttgacggtg tccccgaact gggttggggt aaagggaaga
     1681 actgcacgtt catcggcgct ctggaggcgg ccttggcggt gaccgatcat cccgcgaagt
     1741 attcagatct gatgggatgg tcgggaatgg cctttcgcgt gaggtggtat gtcggcaatg
     1801 acaacagtgg tgcttgcccc tccgcagcgg ttggggagtt cgaggatgaa actgccgctg
     1861 tccagaaata ctcaggttgg tcagctttcg ccgatttcga ccctacgccc tcggcattgc
     1921 ttcgtttcaa agacaaaatt actgcctcaa ttgacaaagg aatgcccgta ttgctctacg
     1981 accctggctg ggatgtcgcc gtcgcctatg gctatgagaa gggtggtgac aggttgtttc
     2041 tgcgaagtta tacagacaac ttttcgcaga tcgatctagc ttcttcgcca gccctcatat
     2101 acatcaatga gaaatatgca gaacctcctt ccaggcgaga cgctttcata gacgcgctca
     2161 atctggccgt ctcgaactgg catcgcgaag cgatggtgcg tgagaaaggt caatatctct
     2221 acggagaact cgggtttgac aaatggatgc aggactatac caacttcaag tcactctctg
     2281 aggaagcgca aaagacaact gcccacgcga gccgcttctg ttacatgacg ctcttggact
     2341 gccgagcagc agcggcgacc ttcctcgatg agcactcaga tgccgtgaat gaggaagcaa
     2401 ggatttgcct gaaacgagct gcggatatct gtcgaagaca actgcctgac gaaatcgcaa
     2461 tgggaacgcc gaatggcgtg ccagagcatc tgctgaaggc caaggaagag ttctccgagg
     2521 tcatcgctga gatcggaaag gctttggcgg cggagcgaca ttgaacgatc ccatccagca
     2581 gcagcagccg atgtgtcagg ccgtctctcc gttgccgtct gttgacgagc aattctaaga
     2641 tgagaggcag gaatgacacc tacgcttgtt ccacagttcg catcgctgag gcgcggatcg
     2701 ctctcagctg gcgatgcacc caaaaaagtc caatcgtgcc ggctagcaga tcggtgagga
     2761 gccttccgaa gaaaacaccc tgcaccccaa agaagtacgc acccgaaacc gtgagtggaa
     2821 caaggaacac caagacccga agagcgttca atgcagttga aaaagcaggg tgatgcagtc
     2881 ccgtgaagaa aaaaccgcag taacgatgca cttccatcat gccatagccg aaagagatga
     2941 ttcggacata agcaacaagg acattgacaa caccagggtc tttgctgaaa acagctgcaa
     3001 gccaaggagc gccgcaaaag aacacgacag caacaccgat gccatagagc atggcaaaac
     3061 gcgtggtgac ctgcaatgct tcctggattc gatcgagacg ctttgcgccg aagttttgac
     3121 tgacaaatgg ggtgagagag attccgagcg ccattgggat catgaacgca aacatttcga
     3181 tccggcccgc ggctccgacg gcggcaaccg gttcattgcc aaagcggccg agcagccggg
     3241 tgataacgcc cgccgagatt ggcatcaaca tcatgctcaa catgctcgga acggcaaagc
     3301 ggagaattcg tcttgccgtg tcgcgatact cacgcagtcg ccattttcgg aagacaagca
     3361 gatgatg
//
LOCUS       DAOQUC010000266         3363 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_32378_length_3363_cov_2.954655, whole genome shotgun sequence.
ACCESSION   DAOQUC010000266 DAOQUC010000000
VERSION     DAOQUC010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3363
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32378_length_3363_cov_2.954655"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1391)
                     /locus_tag="PKH07_05805"
     CDS             complement(<1..1391)
                     /locus_tag="PKH07_05805"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNT34499.1"
                     /translation="MTQRKTSIWIVLAVVFFSAFVLGRWTVPKSPSGRSDDSVDSDTD
                     ISDRVTLWTCSMHPQIQQPRFGQCPLCGMDLVPVASDEGQTGMGEWTLTLSEHSSRLA
                     EIVTSPVERKSVTSEVRMVGKVDFDEGRVRTIAAWVPGRLDRLFVDYTGVPVKKGEHL
                     VSLYSPDLVNSQEELLQSIKALSTLKSPGLESFRETAQKTVAAARERLRLWGLTQEQI
                     DEVERSGKPTTQMTITAPISGIVIQKSVTEGSYVEVGTEIYTIADLSQVWIRLEAYER
                     DLVWIRYGQRVEFTSEAYPGEVFDGKIAFIDPLLNDETRTVGVRVNADNRRGLLKPGM
                     FVSATVRSNVVSDGRVMEPSLAGKWICSMHPEIVKQDSGACDICGMALVRPESLGYAV
                     AGSGRAELPLVIPISAALLTGKRAIVYVKVPDRSGDFEGREVVLGPRAGDEYIVRGGL
                     SEGELVVTNGAFK"
     gene            complement(1446..2084)
                     /locus_tag="PKH07_05810"
     CDS             complement(1446..2084)
                     /locus_tag="PKH07_05810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34500.1"
                     /translation="MRASTSNKLNREYSCDFNGNNSYSRRWCVQTGLLLLLLMAFAPW
                     ECSAIPERVDLIPNGDTFRCLSCHVVVGGALNPFGEAVKQLVDEGSTDPFWSAELAET
                     DSDGDGLSNGEELQDPRGLWQPGQDAPGDGTRVTNPGIAEGKPVAGSGRYRFARYVEP
                     LGISTFCLMVLTALAGFFMPKNRKLLFRWHKRLAVTTILFALSHVLCVILSH"
     gene            complement(2053..2469)
                     /locus_tag="PKH07_05815"
     CDS             complement(2053..2469)
                     /locus_tag="PKH07_05815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34501.1"
                     /translation="MMKGLVFGLLIMIMVTGLFVAGCGKKTEPAKESQASAKPQVKEE
                     NRSGSVGEEVESNAAAEPEPAESKEIALEDLKAPDGSQAGCPVTGGKLNKDIWCIYNG
                     MRIYFENAEAQLDFEGDPEKYVRRLKDAGVNIEQAQ"
     gene            complement(2558..>3363)
                     /locus_tag="PKH07_05820"
     CDS             complement(2558..>3363)
                     /locus_tag="PKH07_05820"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNT34502.1"
                     /translation="TTGMAGYGDIIRAELERARLQDELASLKDGMVPLLARINRELNR
                     SSRAPIAAPDQIEDCTIELPSERTPRQVFGTLELLMAEEAVEREQIGIALARKKSLPD
                     VELGIGFREIESSDMPMSDNGRDPVLGMVSVNLPIRQSKYKAMQREAEQKSQMAIAER
                     DDLQNRLVMELEMILYDYRDARRKLALYEQELIPKAQKVVSVTQQAFATGKVDFLKLI
                     DTQRVLLDLQLNREQALTDSAKSIVRLEVLSGRDYADLDEWLELISRGQ"
BASE COUNT          812 a         1050 c          752 g          749 t
ORIGIN      
        1 ttcttgaaag cgccatttgt gaccacaagt tcgccttcgg atagccctcc tctgacaatg
       61 tattcatccc ccgcacgagg tcccagaacc acttcacgtc cttcaaaatc accagaccga
      121 tcgggcactt tcacatagac gatcgccctt ttgccagtca gcagcgccgc agagatcgga
      181 atcaccaatg gcaactcagc tcgccctgag cctgccactg catatccgag agattcggga
      241 cgcaccagtg ccatcccgca gatatcacat gcgccggagt cctgcttaac aatctccgga
      301 tgcatggaac aaatccattt tcctgcaagt gacggctcca tcacccgacc atctgagact
      361 acatttgagc gcaccgtcgc actgacaaac atacccggct tcagcagtcc cctgcgattg
      421 tccgcgttga ctcgaacacc aacagtgcgc gtctcgtcgt tgagcagtgg atcaataaaa
      481 gcaatctttc cgtcgaaaac ttcaccggga taggcttcac tggtgaactc aacgcgttgt
      541 ccgtaacgaa tccagacaag atctcgttcg taggcctcga gtcggatcca aacctggctc
      601 agatcggcga tggtgtagat ctccgttcca acttcaacgt aactgccttc cgtcacggac
      661 ttctggataa cgataccgct tatgggagcg gtgatggtca tctgcgttgt gggcttgcct
      721 gatctctcga cctcatcaat ctgttcttgc gttagtcccc aaagtcgaag tctctcccgt
      781 gccgcggcaa cagtcttctg cgccgtttca cgaaaggact ccaatccagg agatttgagt
      841 gtcgagagcg ccttgatgga ttgcagcaac tcttcttgag aattgaccag atcggggctg
      901 tacagtgaaa ccagatgctc ccccttcttt acaggtaccc ccgtgtaatc cacgaaaagc
      961 cgatccagtc tgccaggaac ccatgccgcg atagtgcgaa ccctgccttc atcaaagtca
     1021 accttgccga ccatgcggac ttccgacgta acggatttcc gctcgaccgg agaagtgacg
     1081 atctccgcca gccggcttga gtgttctgaa agcgtcagag tccattcccc catgccggtc
     1141 tgcccttcat cactggcaac aggcaccaaa tccataccac agagaggaca ctgaccgaat
     1201 ctcggctgct ggatttgagg atgcattgaa caagtccaga gcgtgacacg atccgagata
     1261 tccgtatcag aatcaacaga atcatccgaa cgtcccgaag gagactttgg aactgtccag
     1321 cgtcccaaga caaaagcaga aaagaacaca acagcaagaa ctatccagat agacgtcttc
     1381 cgttgagtca ttgtctgttc ctctttctga cagcaggtct gccgtgcgat agccgggacg
     1441 ccgtcctaat gactgaggat gacacagaga acatgagaca aagcgaagag aatggttgtc
     1501 acagccagac gcttgtgcca gcgaaaaagc aattttctgt tcttaggcat gaagaaccct
     1561 gccaaagcag tcagcaccat aagacagaat gtggatatgc ccagaggttc cacatagcgt
     1621 gcaaatcggt agcgcccact acctgcaaca ggcttcccct cagcgatccc cggattggtg
     1681 acccgggtgc catcacccgg cgcgtcctgc cccggctgcc acagtcccct gggatcctgc
     1741 agctcttccc cgttggagag accatcccca tccgaatcag tctccgccag ttcggcactc
     1801 cagaatggat ccgtcgaacc ctcatccacc aactgcttga ccgcttcacc aaagggattc
     1861 agcgctccac cgacaacaac gtgacaactc aggcatctga acgtgtctcc gttcggaatc
     1921 aagtccaccc gctccggaat ggcagagcac tcccaaggcg caaaggccat aagaagaagc
     1981 aacagaagcc ccgtttgaac gcaccagcgt cgagaatagg aattgttacc gttgaaatcg
     2041 caagagtact ctctattgag cttgttcgat gttgacgccc gcatctttca acctcctgac
     2101 gtatttctcc ggatcccctt cgaaatccag ttgtgcctcc gcgttctcaa agtaaatgcg
     2161 cattccatta tagatacacc agatatcctt gttgagtttc ccaccggtga caggacaacc
     2221 agcctgtgaa ccatccggtg ccttcaagtc ctccaacgcg atttcctttg actcagcagg
     2281 ttctggctca gccgccgcat tcgactcaac ctcttctcca actgaaccag accgattttc
     2341 ctccttgact tgaggctttg cagaagcctg actctctttg gctggttctg ttttcttgcc
     2401 gcaccccgcc acaaaaagac ccgtcaccat gatcatgatc aacaatccga aaaccaatcc
     2461 cttcatcatt gccatccttt cgaatcaact gctcaatgct ggaccaatgc agcaacacag
     2521 gcacttcaac cgtctctgca acccgcccgc agaaaaacta ctgtcctctg ctgataagtt
     2581 ccagccattc atccagatca gcgtagtctc gtccactcag tacttccaac cgaacaatgc
     2641 tcttggcgga atcggtcaga gcctgctctc ggttcagctg gagatccaaa agcactcgct
     2701 gcgtatcaat caacttcaga aagtccacct tcccggttgc gaaggcttgc tgcgtaacac
     2761 taaccacctt ttgtgccttg ggaatcaact cctgttcgta gagcgcaagt ttccgccgag
     2821 catcgcgata gtcatagaga atcatctcca actccatcac gaggcgattc tgcaggtcgt
     2881 cacgctcagc aatcgccatc tgactcttct gctccgcctc gcgctgcatc gctttgtact
     2941 tgctctgacg gattgggaga ttcacagaga ccatacccag gacaggatcg cgtccgttgt
     3001 ccgacatggg catatccgaa ctctcgatct ccctgaaccc aattcccaat tccacatcgg
     3061 gcagagattt ctttcgcgca agagcaatcc cgatctgctc cctctcgaca gcttcctctg
     3121 ccatcaacag ttccaaggtg ccaaagacct gcctgggagt ccgctcggaa ggaagctcga
     3181 tagtacagtc ctcaatctga tcgggagccg caatgggtgc ccgcgaagag cgattcagtt
     3241 ctctgttgat ccgagccaga aggggcacca tgccatcctt cagcgaagcc agctcatcct
     3301 gaagccgcgc tcgttcgagt tctgcccgaa tgatatctcc atagcccgcc atacccgtcg
     3361 tat
//
LOCUS       DAOQUC010000267         3360 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_32416_length_3360_cov_2.934342, whole genome shotgun sequence.
ACCESSION   DAOQUC010000267 DAOQUC010000000
VERSION     DAOQUC010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3360
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32416_length_3360_cov_2.934342"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(11..322)
                     /locus_tag="PKH07_05825"
     CDS             complement(11..322)
                     /locus_tag="PKH07_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018682685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HNT34503.1"
                     /translation="MVELLERHLNEIRLLCERHHVAKLEVFGSAADASFDPERSDIDF
                     LVEFVPGQSLGPWMAHYFDLQKDLQDLLGRDVDLVMASSMKNPWFRREAKKTRRTLYA
                     A"
     gene            complement(411..1373)
                     /locus_tag="PKH07_05830"
     CDS             complement(411..1373)
                     /locus_tag="PKH07_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018932336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HNT34504.1"
                     /translation="MKVGVFLALFGGQTLEKALDYVIAQGLDTVEVGAGNYPGDPHCK
                     PSVLLKDATALEKFKKTFADKGITISALSCHANPLHPNKAYADAHRKVQRETILLAEK
                     LGIRRVITFSGCPGGGPDDKVPNWITCPWPDDFSKAVAWQWEERVFPFWTDEAKFAKD
                     HGVKVCLEMHPGFVVYNPETMMKLRKEAGPMIGANFDPSHLFWQGIDPIVAVKNLGKA
                     IFHVHAKDTRVDPQNTAADGVLDVKSYGDEINRAWVFRTVGYGHGPEFWKDFVSTLRL
                     VGYDDVLSIEHEDSLMSVNEGFTKAVQFLKGLVLEEKRGAMWWA"
     gene            complement(1459..2616)
                     /locus_tag="PKH07_05835"
     CDS             complement(1459..2616)
                     /locus_tag="PKH07_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNT34505.1"
                     /translation="MKEIRIGMIGYSFMGKAHSNAYRQVAAYFDLKAKPVMKAICGLY
                     EHEAKAAAQKYGWEGYEKRWQDLIARDDIDVIDITTPNNVHCVQAIAAAKAGKHILCE
                     KPLASTLAEAKAMLAAAQKAKVKHMVGFNYRRVPALALAKKMIEKGDLGKIFHIRAVY
                     LQDWIIDPEFPLVWRLVKKEAGSGALGDLGAHIIDVAHHLVGDIDSLTATTETFIKER
                     PLPPKEYGGLAAKAASGKKGKVTVDDAALFLARFKNGALGTFEATRFAAGNKNGNGFE
                     INGSKGTLKFNFERMNELEYYNREDPADRQGFRTILVTEGCHKYAGAWWPPGHILGYE
                     HTFVHEVYDFIENITNNTKPSPDFADGVKVQAVLDAVEQSAKSKQWVKVKV"
     gene            complement(2689..>3360)
                     /locus_tag="PKH07_05840"
     CDS             complement(2689..>3360)
                     /locus_tag="PKH07_05840"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNT34506.1"
                     /translation="TNLGTPWMEREGTSSVWDVKAVIYAAGATPRMVNVPGEKELTGR
                     GVSYCATCDAPFFRDKEVVVIGGGDTALDEGHYLTRFAAKVTIVHRREQLRAEQILQD
                     RAFQNQKIHFELPYLPVEIQGDSKVEGLQIRHRDSGEMKTIPCEGVFVFVGYDPSTGL
                     LARLADLDDGGYVKTDDNMRTKVPGLFACGDCRSKLLRQAITACGEGATAAVAASHYV
                     ESLSH"
BASE COUNT          766 a         1007 c          769 g          818 t
ORIGIN      
        1 cttcggtgtc ttaggcagca tagagtgttc tccgagtctt ctttgcctct cgcctgaacc
       61 aagggttctt catcgaggaa gccatgacca gatccacatc tcgccccaag agatcctgca
      121 ggtccttctg aagatcaaag tagtgtgcca tccacggacc caaactctgg cctggcacga
      181 actcaaccaa gaagtcaata tctgacctct cgggatcgaa cgaggcatcg gctgctgagc
      241 caaagacttc cagtttggct acatgatgtc gctcacagag taatcgaatc tcattgagat
      301 gcctctccag aagttcaacc acccaacagc ctcctcgttg tcagcacagg atccagacca
      361 tttcagcaga tgctgattgg gagcaatggc acactgtcag tcaggctttc ttacgcccac
      421 cacatcgcgc cgcgcttctc ttcaagcaca agtcccttta ggaactggac ggctttggtg
      481 aagccttcgt tcacactcat caggctgtct tcatgttcaa tgctgagtac atcatcatag
      541 ccgacaagtc gtagcgtcga aacaaagtcc ttccagaact ccggaccatg cccgtagcca
      601 accgtcctga aaacccacgc tcgattgatc tcgtcaccgt acgacttgac atcgagcaca
      661 ccatccgccg ctgtattctg aggatccacg cgagtgtcct ttgcgtggac atggaaaatc
      721 gccttgccaa ggttcttgac tgcaacgatc gggtcaatcc cctgccagaa cagatggctg
      781 ggatcaaaat ttgcaccgat catcggtcca gcttccttgc gcagtttcat catcgtttcg
      841 ggattgtaaa ccacaaatcc gggatgcatc tccaagcaga ccttcacacc gtgatccttg
      901 gcaaacttcg cttcgtcagt ccagaagggg aatacgcgct cttcccactg ccaggcaacg
      961 gctttcgaaa aatcatcagg ccatggacag gtgatccagt tcggcacctt gtcatccggt
     1021 ccaccgccag gacatcctga gaacgtaatc actctgcgaa ttccgagttt ctcagccaaa
     1081 agaatcgtct cacgctggac ttttcgatgc gcatcggcgt aagccttgtt cggatgcaac
     1141 ggattggcgt ggcaactcag tgcggaaatc gtgatgcctt tgtctgcaaa agtctttttg
     1201 aacttctcca gcgctgtggc atccttcaac agcacagaag gtttgcagtg cggatcacct
     1261 ggatagttcc ctgccccaac ctcaactgtg tcaaggccct gcgcaatgac ataatccaaa
     1321 gccttttcca aggtctgccc accaaacaat gcgagaaaca ctcctacttt catttttgcc
     1381 tcctatttat cttcctctgt ccccaaaggg gaaacagggc gattcagcat cctgtgataa
     1441 gtccatgtcc cttgcgggtt agaccttcac cttgacccac tgcttgctct ttgcagactg
     1501 ttccaccgcg tccaaaaccg cctgcacttt gacgccatcg gcaaagtctg gtgacggctt
     1561 ggtattattg gtaatgttct cgataaagtc atagacttca tgaacaaacg tgtgctcata
     1621 ccccagtatg tgtcctggag gccaccaggc acccgcgtac ttatgacaac cttcggtcac
     1681 aagaatcgta cggaaccctt gacggtctgc gggatcttca cggttgtagt actccagttc
     1741 attcatgcgc tcaaagttga acttgagtgt tcccttgctg ccgttgatct caaaaccatt
     1801 gccattcttg ttgcctgctg caaatcgtgt cgcttcaaag gttccaagcg ctccattctt
     1861 gaatcgagcg agaaacagcg cggcatcgtc cacagtgact ttgcctttct ttccactggc
     1921 ggcttttgcg gcgagacctc cgtactcctt aggcggcaaa ggtcgctctt tgatgaacgt
     1981 ttccgtagtc gccgtcagtg aatcaatgtc tccgacgaga tgatgcgcca cgtcaataat
     2041 atgtgcgccc agatctccca atgcacccga accggcttcc ttcttgacga gtctccaaac
     2101 cagcgggaat tccggatcaa tgatccagtc ttgaagatag acagcacgaa tgtgaaagat
     2161 cttccccaaa tccccctttt caatcatctt ctttgccaat gccagcgcgg gcacacggcg
     2221 atagttgaac ccgaccatgt gcttcacttt tgctttctgc gccgcggcaa gcatcgcctt
     2281 cgcttccgcc aaggttgaag ccaaaggctt ctcacagaga atatgcttgc ctgcctttgc
     2341 cgcggcgatc gcttgcacac agtgaacgtt gttgggagtg gtgatatcaa tgacatcaat
     2401 gtcatcacgg gcgatgagat cctgccaccg cttctcataa ccttcccagc cgtacttctg
     2461 cgctgcggct ttcgcctcat gctcatacag gccacagatc gccttcatga caggcttggc
     2521 tttgagatca aagtaggcag ccacctgccg gtaggcgttg ctgtgcgcct tacccataaa
     2581 actgtaccca atcatcccaa tgcgaatttc tttcacggcc agacctcctt ggtgggaaca
     2641 accccaccga atgccttttt gttctatcct ccagccagtt gggctgactt aatgactcaa
     2701 ggactccacg taatgagatg cggcgaccgc tgccgtggcg ccttctccac aggcggtgat
     2761 ggcctgcctg agaagtttgc tgcgacagtc cccacaggca aaaagcccgg gcactttggt
     2821 tcgcatattg tcgtccgtct tgacgtatcc gccgtcgtca agatccgcaa gccttgccaa
     2881 caagcccgtt gacgggtcat atccgacaaa aacaaaaacc ccttcgcagg ggatggtctt
     2941 catctctcca gaatcgcgat gtcgaatctg aagaccctca accttgctgt cgccttgaat
     3001 ctccacagga agataaggca actcgaaatg aatcttctga ttctgaaacg ccctgtcctg
     3061 aagaatctgt tccgctcgaa gttgctcccg acgatgcaca atggtgactt tggcggcaaa
     3121 acgtgtcagg tagtgtccct cgtcaagcgc cgtatcaccc ccaccgataa cgacaacttc
     3181 cttgtctcgg aaaaagggtg catcacatgt ggcgcagtaa gaaacaccac ggccggtcag
     3241 ttccttctca cctggcacat ttaccatgcg cggggtagcc cctgcggcgt agatcacagc
     3301 cttcacatcc cagacgctgc tggttccctc gcgttccatc cacggggtgc ccaggttggt
//
LOCUS       DAOQUC010000268         3359 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_32427_length_3359_cov_3.480327, whole genome shotgun sequence.
ACCESSION   DAOQUC010000268 DAOQUC010000000
VERSION     DAOQUC010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3359
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32427_length_3359_cov_3.480327"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..476)
                     /locus_tag="PKH07_05845"
     CDS             complement(<1..476)
                     /locus_tag="PKH07_05845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34507.1"
                     /translation="MLGRMSLLSLILCLSSGLCLAQEDVLTQGTGKTQQGKLIGYSQD
                     SFDFAVQQGGGIFLGQLHPAEQVANIVVSSSEDQIYLLCQELASDPVLQGASIETPDR
                     PLDVLQTKTNETRTGRVEGYTDGIVFFRTSDPREADAVDLIPIEEIESFALSNADF"
     gene            complement(584..1345)
                     /locus_tag="PKH07_05850"
     CDS             complement(584..1345)
                     /locus_tag="PKH07_05850"
                     /inference="COORDINATES: protein motif:HMM:NF038353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxLYD domain-containing protein"
                     /protein_id="HNT34508.1"
                     /translation="MSYAVKFQDLFPHRGRCGEWLGLCSYKTPWLYCLSGVLIFVFCC
                     VAVMLLLPAVAVQGDEVLQTDGTKLSGRILSFDGRNLTVQSASGQVRSIPMVDVASFV
                     VDPSAELIRRIDKVQQDLDQIGRMVDNLGASWQEGRDQIRADVTDLNPMTQVVISKKS
                     GFFRKSTFVIEGEVRNRSSVFIRDVHIRATFYNREGVPVNEVVFPTLVSTISPGSGAK
                     FQKDIPNAPRFETFELTLLEGRPQTTERRGGAQKR"
     gene            complement(1377..1601)
                     /locus_tag="PKH07_05855"
     CDS             complement(1377..1601)
                     /locus_tag="PKH07_05855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34509.1"
                     /translation="MKFKCPSCFKVAEFSQEEIRKIFLNCPECSRQFRPSECSPVISA
                     DKKQDKAIRRAFTSVNRELIKEALAAQKSK"
     gene            complement(1914..>3359)
                     /locus_tag="PKH07_05860"
     CDS             complement(1914..>3359)
                     /locus_tag="PKH07_05860"
                     /inference="COORDINATES: protein motif:HMM:NF021254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archaeosortase/exosortase family protein"
                     /protein_id="HNT34510.1"
                     /translation="MMGVRTMGNAHPFCNRTVLFFRLGLTLVVMVTCFPMLQWVYLRW
                     LSPTGSFLCAAVLTATLFGWFRILLSDGESLTPIQSLRWLTGSCVFLIAFAVVYPYVP
                     GLVSSGLGACALSCMMMAVLPEVERGRSWAIPILVILSIPLTPSLQFFLGSPLRFLVA
                     RCTALMSGMSIRAIGVGLSDGHHTVFIDAPCSGIRMLTTSVLLASGTSFYLRLSRCRT
                     FLVMLFGVFLAVAGNVLRAGALFLMETRLPLGAWAHTWIGVIVFAFCAFSLVWLARAV
                     ERGDTARVGSPRPMDSLTYRVAVFVILLVAISSTIILWKPNRVTAETRGIRSDVVLPT
                     TWGGTGLDPLPLQSDVREILKGFPGQVAQFRLGDSDTVVLLRISHRATRDMHPAEDCY
                     RVLGWKCNPEPAWMDQSGHTWSSFSVEKPDGSRRLVRQCYFSVALDPGFTDMEHLLAK
                     AVSWPDASSWYWAAARPGSTVRTTLAVTVEE"
BASE COUNT          907 a          934 c          803 g          715 t
ORIGIN      
        1 tcaaaatccg cattggagag tgcgaaggat tcgatttcct caatgggaat cagatccacc
       61 gcatcagcct ctctcggatc cgatgtccgg aaaaagacaa ttccatcggt atagccttcc
      121 acccgaccgg ttctcgtctc attcgtcttg gtctgaagga catccaacgg cctgtcaggc
      181 gtttcaatgc tcgccccctg aagaacagga tccgacgcca attcctgaca aagcaggtat
      241 atctgatcct ctgaagaaga aacgacaatg tttgccacct gctccgcggg atgcagttga
      301 cccagaaaaa tgcctccccc ctgctgaacc gcaaagtcga aactgtcttg agagtagcca
      361 atcagttttc cctgttgcgt ctttcctgtg ccttgcgtca acacatcttc ctgggcaaga
      421 cacaagccac tcgacagaca cagaatgagt gacagcaaag acattcttcc taacatgatg
      481 cttcctttcc aatctcttct gcccttgcct ctgtggcaag gcggacactc ttcatctccc
      541 cccccgtacc tgggaagtga tgagtccttg aacaccgttc tctctaacgt ttctgggcac
      601 ccccacgtcg ctctgttgtc tgaggtcttc cttcaagcaa ggtcaactca aaggtttcga
      661 agcgtggggc gttgggaata tctttctgaa acttggcgcc acttccaggg ctgattgtcg
      721 agaccaacgt gggaaagact acctcgttca ccggcacacc ctcacggttg tagaaggtcg
      781 cccgaatgtg gacatcccgg atgaacacac tggacctgtt tctaacctct ccctcgatca
      841 caaacgtact tttccgaaaa aaaccgcttt tcttgctgat gacaacttgg gtcattggat
      901 tgaggtcagt cacgtcagcc cgaatctgat cccgcccttc ctgccaggac gctcccagat
      961 tatcaaccat tctgccgatt tgatccaggt cctgttgaac cttgtctatc ctacgaatga
     1021 gttctgcact tgggtcaacc acaaaggaag cgacgtcaac cataggaatc gaacgaacct
     1081 gaccggaagc agattgaacg gtcagattcc tgccgtcaaa ggagagaatc cggccggaaa
     1141 gtttcgtgcc gtccgtctgt agaacctcgt caccctgaac cgcaacagca ggcaagagaa
     1201 gcatcaccgc aacacaacag aacacaaaga tcaggactcc cgaaaggcaa tacaaccatg
     1261 gagtcttgta cgaacaaagt cccagccact ctccgcaacg gcctctgtgt ggaaatagat
     1321 cttggaactt caccgcatac gacatgtcaa gaccaccttc ttcgttgaca atgaggctat
     1381 tttgacttct gtgcggcaag agcctctttg atcagttccc gattgactga tgtaaatgca
     1441 cggcgaatcg ctttgtcctg cttcttgtca gcggagatca caggggaaca ctcacttggg
     1501 cgaaactgcc ttgaacactc agggcaattc agaaatatct tgcggatctc ctcttgcgag
     1561 aactcggcaa ccttaaagca acttggacat ttgaatttca tacctctacc ccctgattat
     1621 ttgatcagtc atttctcgaa tgcgcccata atgcctcata ttcaaactaa ccctttatgg
     1681 ccatcggtgt caacttcttt tgcaggatgg cttactggct cagctacgta ggggttggtg
     1741 aggaaggaca ttgaagcgcc ttctagatac aggcgttggg agaaacaagg ctatggattg
     1801 tgagagtaag actctggatg tgttcaccct ggacctgaac gcaccgccag ttggatggaa
     1861 taatcacaag cgatgcagta ctggtctgag agaagaaata cggtttcgga ttctcattcc
     1921 tcaaccgtca cagccagagt cgttctaacc gtggagccgg gacgagcagc agcccaatac
     1981 cagcttgagg catctggcca tgaaacagcc tttgccagaa ggtgctccat gtcggtgaac
     2041 cctggatcga gagccacaga gaaatagcat tgccggacaa gccttctgga accgtctggc
     2101 ttttcaactg aaaatgagct ccatgtatga cccgactgat ccatccaagc cggttcagga
     2161 ttgcatttcc atcccaagac tcggtagcaa tcttcagcag gatgcatgtc tcgggtagct
     2221 cgatggctga tcctgaggag aacaaccgta tccgaatcgc ctagacgaaa ctgcgccact
     2281 tgccctggga accctttcag tatctctctg acatcgctct gcagtgggag aggatctaag
     2341 cccgtgcctc cccaagtcgt gggcaggaca acgtccgaac ggattcctcg ggtctctgca
     2401 gttaccctgt tcggtttcca gagaattatg gtgctcgaga tagcaacgag aaggataacg
     2461 aagactgcaa ctcgatatgt caacgaatcc atcggtcttg gtgacccaac tctggcggtg
     2521 tctcccctct cgacagcacg agccaaccag acaagagaaa aggcgcagaa cgcgaaaaca
     2581 ataactccaa tccaagtgtg cgcccaagca cctaacggaa gccttgtctc cataagaaac
     2641 aaagcacctg cccgaagaac attaccagcc acggccagga aaactccgaa aagcatgaca
     2701 agaaacgtcc tgcacctcga tagacgaagg tagaaggagg tcccggatgc caggagcact
     2761 gaggtggtca gcatgcgaat tccactgcac ggcgcgtcta taaaaactgt gtgatgaccg
     2821 tccgacagtc caacgcctat cgcccgaatg ctcatcccgc tcatgagagc cgtgcatctg
     2881 gctacgagga aacgcaacgg actgccaaga aaaaactgta gagacggagt cagaggtatc
     2941 gaaaggataa caaggattgg aattgcccat gagcgcccgc gttcgacttc ggggagtacg
     3001 gccatcatca tgcacgaaag agcacaagcc cccagaccgg atgaaacaag accgggaacg
     3061 tatggataga caacagcaaa agcaatcaga aagacacaac tcccggtcag ccaacgcaac
     3121 gactggatcg gagtcagact ctcaccatct gacaggagaa ttctgaacca gccgaaaaga
     3181 gtggctgtca agacagcagc acagaggaat gagccagtag gtgagagcca acgcaaataa
     3241 acccactgaa gcattgggaa gcaggtcacc atgaccacca acgtaagccc cagtcggaaa
     3301 aacagtactg tccgattaca gaaggggtgt gcgtttccca tcgttcggac acccatcat
//
LOCUS       DAOQUC010000269         3355 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_32486_length_3355_cov_3.570000, whole genome shotgun sequence.
ACCESSION   DAOQUC010000269 DAOQUC010000000
VERSION     DAOQUC010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3355
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32486_length_3355_cov_3.570000"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1144)
                     /locus_tag="PKH07_05865"
     CDS             complement(<1..1144)
                     /locus_tag="PKH07_05865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34511.1"
                     /translation="MESTKHPGARWDGIPSLRDLLAVVFKHKLGSAILFLIIVSVVTT
                     ITYVRPFMYRASSKILIKFERDEVPLSRTSQVSRPILTMRSAEEDIRTEMEILSSKHL
                     IEAVVNRLASDLSGSGHPEPTTFWARTRSQIAKIMGTAFQGILQVGYRLGLMRELTPT
                     ESLLRSVQQNLEIEAIGDSNVIRISYSSSRPDLCAKVVNTMTELYLEQHLNVHKTPRA
                     QEFFTEQKTLWEERLGAQEKEILEFREKWSLSSIELERGTLLESISTAMMENDRLQAE
                     IEDAEERRDSLKKQMTERTNYIEPDKVSPALLAVDTVYRNLDNERLMTESTLQGLVAK
                     SQLYEKQLQSYRNRLDVLDQNEIQLKGMDRELAIFEDNYKTYCGKLE"
     gene            complement(1213..2115)
                     /locus_tag="PKH07_05870"
     CDS             complement(1213..2115)
                     /locus_tag="PKH07_05870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34512.1"
                     /translation="MKTIAFGKGIRTETVARMLLACVLAVCLTGCEALNALSQGAPVT
                     VSTDWSAPEVLPSPVNSPEWEDSPSITPDGKTLFFTRGRDRDVETYLTHKTETGWSEP
                     EPLDKINLKSFPTGGAHTQDGKTLFLASFRPGGLGQADIYKCQNVDGVWTNLENIGAP
                     VNTEHMESEPYISPDGKTLYFASNRTGNTDIWFCTRAGDSWTEPENMGPPINTDKDET
                     QPFLTRDGKQLYFMAVNRNGIGGPAIFHSVKRNDHWQTPTLVVSGMVGEPTLTADERY
                     LYFVHIIMSASQLTDAEIMVARRK"
     gene            complement(2129..>3355)
                     /locus_tag="PKH07_05875"
     CDS             complement(2129..>3355)
                     /locus_tag="PKH07_05875"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNT34513.1"
                     /translation="GSQILGGLKAARIVKQYSKVPVVWGGVHSSIFPTLTLKDDLVDI
                     VVRGEGEVTFYELVKRLQEGHSLDGLQGVWFKRDGQIVENPERDFINMDDFPDVPYHL
                     VNLNDYLHQFFGQRRVLEVESSRGCPYACAFCYNPLYGKRKWRALGAENVIGRIQTLV
                     DRNKVRCFHFIDDGFFIDKDRVRQIMEGIVHRGLEIKLGFQGARIDTLRKMSDEDMNS
                     LCRAGGTFLQFGVESGSPRILQLINKQIEVGEVIELNRRLARFPSLIPYYNFMCGFPT
                     ETREDLFMTTSLAWQLLAENRQAMISPFHHYKPYPGTPLAKIATDENYITGKSLEEWG
                     HFDWTEGIKHERDKSSLRLLKNVEMVSIFADRKMESQSDSRLWAFLGSLYRPIARLRL
                     RHNIYSFMPEGRLLRH"
BASE COUNT          734 a          900 c          836 g          885 t
ORIGIN      
        1 cttccagttt gccgcagtag gtcttgtagt tatcctcaaa aatcgccaac tcccgatcca
       61 ttcccttcag ctgtatttcg ttctgatcaa gcacatccag acgatttcgg taggattgca
      121 gctgcttttc gtagagctgg ctctttgcta caagcccctg aagtgtggat tcggtcatca
      181 agcgttcatt gtccagattg cgatagacgg tatcaactgc cagaagagca ggactgacct
      241 tgtcgggctc aatgtagttt gttctctcag tcatttgctt cttgagggaa tcccgcctct
      301 cctcggcatc ttcgatctcg gcctgcagac gatcattttc catcatggcc gttgagatgc
      361 tctccaaaag ggttccgcgt tccagttcga tcgaagagag agaccatttt tcacggaatt
      421 ccagaatctc cttttcctgt gctccaagac gctcttccca aagagtcttc tgttcggtga
      481 agaattcctg cgcccgaggg gtcttgtgaa cattcaaatg ttgttcgagg taaagttcag
      541 tcatcgtgtt gacgactttt gcgcacagat caggacgcga ggaggaatag ctgattcgga
      601 tgacattgga gtctccgatt gcttcaatct caaggttttg ttggacgctt ctcaacagag
      661 attcagtggg tgtcagttcc cgcatcaggc caagtctgta acccacttgg agaattccct
      721 gaaaggcggt tcccatgatt tttgcgattt ggcttcttgt ccgtgcccag aacgtagttg
      781 gttcgggatg acccgatccg gacaggtcgg acgcgagtct gttcaccacg gcctctatga
      841 gatgcttact gctgagaatc tccatctctg tgcggatatc ctcttccgcg ctgcgcattg
      901 tgagaatcgg ccgcgagacc tgactggttc tgctgagggg aacctcatct ctttcgaact
      961 tgatgagtat ctttgaggat gcacgataca tgaaaggtct cacgtacgta atcgtcgtca
     1021 caacggagac gatgatcagg aacaagatgg cagaacccaa cttgtgctta aaaacgacag
     1081 ccagaagatc tctcagtgat gggattccat cccatcgcgc tccagggtgt ttcgttgact
     1141 ccatgctttg actcctgcct tatcaatgat ttgcaggttc gccgtttccg gcgacctgtt
     1201 tgttgatgtc cttcatttcc gtctggcaac catgatctcc gcatccgtga gttgcgatgc
     1261 gctcatgatg atatggacga aatagagata tcgttcgtca gcggtcagcg ttggctctcc
     1321 aaccatccct gaaacgacca aggttggcgt ctgccaatgg tcgtttctct tgaccgaatg
     1381 gaatatcgcg gggcctccga ttccattcct gttgactgcc atgaagtaga gctgcttccc
     1441 atcgcgggtg aggaaaggct gtgtttcgtc cttatcagtg tttatgggag gtcccatgtt
     1501 ttctggttca gtccaggagt ctccggcgcg tgtacagaac catatatccg tattccccgt
     1561 tctgttggat gcgaagtaga gcgtcttgcc gtctggagaa atgtacggct cactctccat
     1621 gtgttccgtg ttgacaggtg cgccgatgtt ctccaggtta gtccaaacac catcgacgtt
     1681 ctgacatttg tagatatccg cttggccaag gccgccggga cggaatgagg ccaggaagag
     1741 tgtctttcca tcctgtgtat gtgctccgcc ggtcggaaag gacttcaggt tgatcttgtc
     1801 caacggttca ggctcactcc aaccagtctc ggtcttgtga gtcaggtacg tttcaacgtc
     1861 tcgatcacgt cccctggtaa aaaacagggt cttgccatct ggagtgatgg atggactgtc
     1921 ttcccattcc ggactgttta ccggagatgg gagcacttct ggtgcgctcc agtcagtgga
     1981 aactgtcact ggagcgccct gtgagagggc attcagagcc tcgcatcccg tcaagcacac
     2041 tgccagcaca caggcaagca acatcctcgc caccgtctct gttcggattc ctttgccaaa
     2101 agcaattgtt ttcattgcat ctgtcctttc agtgtctcag cagccgtccc tcgggcatga
     2161 acgagtagat attatgcctc agtctgagcc ttgcaatggg acgatacagg gaacccagaa
     2221 acgcccaaag cctggagtcc gactgggatt ccatctttcg gtctgcgaat atcgaaacca
     2281 tttccacatt cttcaggagc ctcaaactgc tcttgtctct ctcgtgtttg atcccctcag
     2341 tccagtcgaa atgcccccat tcctcgaggc ttttgccggt aatatagttc tcatcagtgg
     2401 caatctttgc cagaggggtg ccgggatagg gtttgtagtg atgaaagggg ctgatcatcg
     2461 cctgtcgatt ctcagccaag agttgccagg caagcgaagt tgtcatgaaa aggtcttccc
     2521 gggtctcagt gggaaagcca cacatgaaat tgtagtaagg aatcaatgaa ggaaacctcg
     2581 cgagcctacg gttcaattcg ataacctctc ccacttcaat ctgcttgttg atcaactgga
     2641 ggatgcgtgg tgacccggat tccactccga attggagaaa ggtgccaccc gccctgcaca
     2701 gagagttcat gtcctcatca ctcattttcc gaagggtgtc tatacgagca ccctggaaac
     2761 cgagtttgat ctcaagcccc ctatggacga tgccctccat gatctgtctg actcgatctt
     2821 tgtctatgaa gaaaccatcg tctatgaaat gaaagcaccg gactttgttc ctgtcaacca
     2881 gggtttggat ccgcccgatg acgttctccg ctccgagtgc gcgccacttt ctcttgccat
     2941 agagcggatt atagcagaag gcgcaagcgt aaggacaacc gcgagatgac tcaacctcca
     3001 aaacacgcct ctgtccgaag aactggtgca gatagtcgtt cagattcacc agatgatagg
     3061 ggacatccgg gaaatcatcc atgttgatga aatctcgctc ggggttctca acgatctggc
     3121 cgtcgcgttt gaaccacact ccctggagcc cgtccagtga gtggccttcc tgcaatctct
     3181 tgaccaactc atagaaggtc acctccccct cgccacgaac cacgatgtcc actagatcat
     3241 ccttcaacgt cagcgttggg aagatggatg aatgaactcc gccccacacg acaggaacct
     3301 tgctgtactg cttgacgatg cgtgctgcct tcagacctcc aaggatctgc gatcc
//
LOCUS       DAOQUC010000270         3353 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_32513_length_3353_cov_3.257429, whole genome shotgun sequence.
ACCESSION   DAOQUC010000270 DAOQUC010000000
VERSION     DAOQUC010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3353
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32513_length_3353_cov_3.257429"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..406)
                     /locus_tag="PKH07_05880"
     CDS             complement(<1..406)
                     /locus_tag="PKH07_05880"
                     /inference="COORDINATES: protein motif:HMM:NF024923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HNT34514.1"
                     /translation="MKKALVLLAIACLLGFLGFKVNSKVKEVKAKSESNSQKRGATSV
                     AVEVAPVRRATVREIGRFTGSLIPRSSFVVAPKIAGRLERLTVDTGDSVANGQLVAEL
                     EDEEYIQQVQQARAELAVANASVAECKSALEVS"
     gene            654..2099
                     /gene="rpoN"
                     /locus_tag="PKH07_05885"
     CDS             654..2099
                     /gene="rpoN"
                     /locus_tag="PKH07_05885"
                     /inference="COORDINATES: protein motif:HMM:TIGR02395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HNT34515.1"
                     /translation="MHQTLRLSQSLQQAQKLVMSPRMQQSLKLLALPSLELSSLVQQE
                     LVENPVLEEANPTDISSAGMTSADDRILSEGEEVENREELRSGVEFDQDWESYFTDTS
                     DAGPLPTATETGDDEYEPIIVQASSLKEDLLRQLSLSTDDPLMFRVGEVIINLLDDTG
                     YLLQPLEQIAQMLEVSLDDAEKALRIVQTFDPVGVGARDLRECLLIQYEAREETDPVL
                     RSVIEEHIENLEHRRLVKIAAALKTTVEKIQEVADTLANWEPIPGRKYMSSENEYLSP
                     DVFVEKVEGEYQIRINDDGTPPLRISRKYRQMLANKDQLTREEYEFLKSKCSSAIWLI
                     RNIEQRRQTLYKVTKKIMEFQIGFLENGISSLRPLRLQDVADAVGVHETTVCRVVNKK
                     YVQTPRGLFELKFFFSTGLNSDTGDDPASKAVMEMIRSLIEQENPTKPLSDQRISDLL
                     AEKGIKIARRTVAKYRENMDILPTSMRKRVH"
     gene            2195..>3353
                     /locus_tag="PKH07_05890"
     CDS             2195..>3353
                     /locus_tag="PKH07_05890"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNT34516.1"
                     /translation="MSYVLPKRSIALLPIAVIVMAGVFRFVALDRIPSGFFRDEADKG
                     YGAFCLLKTGADAQGRSWPLFISSLSIYTTPLYQYLLIPAVACFGLSEYVVRSPAALA
                     GMLTVAFVFLLVRRWMGFWAAIVASFLLAVSPWHLPFSRWANQGILLPCFLTIFMLVR
                     EKSRERRLWVFGSCLALTGALYSYEPAKVLVPLLLAGLVLHDLFRRDSRDLKSDGILI
                     GVLALSALPMLLFHLSHAEMSNARFSSISVFRGYEGDLLGVTVQIVRNFLEHVSPKYL
                     FISGDANLRHSVASMGQLYLTECIALALACFAVIREKRREDVICIIWFVAAIFPASLT
                     NEGIPHSLRTIGAVVPLTLLSARGFGVMIDYAASKGLSSKVGKGTLALLVAT"
BASE COUNT          758 a          830 c          877 g          888 t
ORIGIN      
        1 tggacacttc cagggcgctc ttgcattcgg caacacttgc attggccact gcaagctcag
       61 cacgtgcttg ctgaacctgc tggatatact cctcgtcttc cagctccgcc accagttgtc
      121 cgtttgccac ggagtctccc gtgtcaacgg tgagtcgttc gagtcgtccg gcaatcttgg
      181 gtgcaacgac aaaggaggat ctgggaatca gcgaacctgt gaatcttccg atctctcgaa
      241 ccgtggcacg gcgtacaggt gcaacctcta cagcgacgga tgtggcaccg cgcttctgag
      301 agttggactc cgacttggct ttgacctctt tgaccttcga attcactttg aaaccaagaa
      361 atcccagaag gcaggcgatg gctagaagta caagggcttt cttcatccga aatctccttc
      421 gctcatacaa acggtatgat ctctctaggc gatgcggaaa acgcttcggt tggtacacct
      481 gatgtacgca acagtgaaat ctatcgactg caggaatctt tcgtccagat tcccaagttg
      541 tcaagaatcg attgcacttt acccaaattg acacctgttg gctttcgtga tagtttgaca
      601 atgaaagaag agagaaagag acttgcgacc acaaagcagg atgttttact ctgatgcacc
      661 agacactgcg actttcacaa agtcttcaac aggctcagaa acttgtcatg agcccaagga
      721 tgcagcagtc cttgaagctg cttgccctgc catccttgga actgtccagt ctcgtacagc
      781 aggaactcgt tgaaaacccc gtccttgaag aagcgaaccc cacggacatc tcttccgcag
      841 gaatgacctc agcggacgat agaattcttt cggaaggcga agaagtcgaa aaccgtgagg
      901 aactcagatc tggcgtcgag tttgaccagg actgggagtc ctattttact gataccagcg
      961 atgcgggacc tcttccgact gcaacagaga ccggagatga tgagtatgag ccgattatcg
     1021 ttcaggcgag ttctctgaag gaagatttgc tccggcagct tagtctctcc accgatgatc
     1081 ctctgatgtt tcgtgtcggt gaggtcatca ttaacctgct cgatgatacg ggctatctgc
     1141 tacaacctct tgaacaaatt gctcaaatgc tggaagtctc tctcgatgat gcagagaagg
     1201 cactccgaat cgttcagacc tttgatccgg ttggtgttgg agccagggat ctccgagagt
     1261 gcctgctgat tcagtacgag gctcgtgagg agacagatcc tgttctgaga agtgttatcg
     1321 aggaacacat tgagaatctt gagcatcgcc gtctggttaa gattgctgcc gctctgaaga
     1381 ctaccgttga gaagattcag gaggttgcgg acacgctcgc aaactgggag cctatcccag
     1441 gacgaaagta catgagcagt gagaatgagt acctctctcc tgacgtgttt gtcgaaaagg
     1501 tcgagggaga gtaccagatt cggatcaatg acgatggaac gcccccactc cggatcagtc
     1561 gcaagtaccg acagatgctt gcgaacaagg atcagctgac ccgtgaggaa tatgaattcc
     1621 ttaagagcaa gtgcagctct gctatctggc ttatccgaaa tatcgagcag cgccgtcaga
     1681 cgctctacaa agtcaccaag aagatcatgg agtttcagat aggatttttg gagaatggaa
     1741 tatcgtcatt gcgtccactg aggcttcagg atgtggcaga cgctgtcggt gttcacgaaa
     1801 ccacggtttg ccgtgtggtg aacaagaaat atgtccagac tcctcgtggg cttttcgaac
     1861 tgaagttctt cttctcaaca ggactgaatt cagatacagg tgacgatccc gcgtccaagg
     1921 ccgttatgga gatgatccgc agccttatcg agcaggagaa tccgacgaaa ccactttccg
     1981 atcagagaat ttctgatctt ctcgcagaga agggcatcaa gatcgcccga cgaactgttg
     2041 ccaagtatcg ggagaatatg gatatccttc ccacaagtat gcgcaaaaga gttcactagg
     2101 tgattctgta tttctatctt ctttggcgaa gaacactaac ggccgatctg cgggagcacg
     2161 tcagggcagc ggtcacccat gtcattgtag ccctatgagt tacgttctac ctaagcgctc
     2221 gattgccctt cttcctattg ctgtcattgt catggcaggc gtgtttcgct ttgtggctct
     2281 ggatcgtatc ccatctggtt tcttccgtga tgaggcagat aaggggtatg gtgctttttg
     2341 cctgctcaag acgggcgcgg atgctcaggg gcgatcatgg cccctcttca tctcttcgct
     2401 ttccatctat acgactcccc tgtaccagta tcttctgatt ccagctgtgg cgtgttttgg
     2461 gttgagcgag tatgttgttc gctctccggc agctcttgca ggtatgctta ctgtcgcttt
     2521 cgttttcctg ctcgtgaggc gatggatggg gttctgggct gcaattgtgg cttccttttt
     2581 gctggcagtg tcaccgtggc accttccttt ttcacgatgg gcaaatcagg gaattctgct
     2641 cccttgtttt cttacaattt tcatgctcgt tcgggagaag tcgcgagaac gaaggctctg
     2701 ggtattcggt tcctgcctcg ctctcacggg tgccctctat tcctatgagc cagcaaaggt
     2761 tcttgtaccg ctcttgcttg ctggattggt tctgcatgat ctcttcagaa gagacagccg
     2821 agatctcaag tctgatggta ttctgatcgg ggtgctagca ttaagtgcac ttccaatgtt
     2881 gctctttcac ctcagtcatg ccgagatgag caacgcgcgg tttagcagca tttctgtttt
     2941 ccgaggatat gaaggagatc tactgggtgt cacagttcag attgtgagga acttccttga
     3001 gcacgtgtca ccgaagtatc tcttcatctc tggagatgca aatttgaggc attcggttgc
     3061 ctcgatgggg cagctctatc tcacggagtg cattgctctg gcacttgctt gcttcgcggt
     3121 cattagagag aagcggcgag aggatgtgat ctgcatcatc tggtttgttg ctgcgatctt
     3181 tcctgcttca ctgaccaacg aggggattcc gcattcgctc aggacaatag gagcggttgt
     3241 tccgttgaca ctcctttctg cgagaggctt tggcgtgatg atcgactatg ccgcttcaaa
     3301 aggcttgtca tcaaaggtgg ggaagggaac tctcgctctt ctggttgcaa cac
//
LOCUS       DAOQUC010000271         3349 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_32563_length_3349_cov_3.996053, whole genome shotgun sequence.
ACCESSION   DAOQUC010000271 DAOQUC010000000
VERSION     DAOQUC010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3349)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3349)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3349
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32563_length_3349_cov_3.996053"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1308)
                     /locus_tag="PKH07_05895"
     CDS             complement(<1..1308)
                     /locus_tag="PKH07_05895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34517.1"
                     /translation="MRNLLIYSLLALVFVVGSASAETVILQEDFSTWPTGALTTASSL
                     TAGWSVVENGLSVTTENATMGGKTVKSTAGSSVGCVRGTWSAVDPTTAAPFRLTLYMR
                     MTGSRTEKGAALKSYDNLFTGCSPMLYFCPNTATNVDYRLRDRENCGGAVPSVYAVKD
                     LYQETADKDKMIVWQILCTDVDAKLYKNGVLVKTFAGPPNGTMDNFQAVEIGVPMNVP
                     GTTFPRGSEYGPNGTFWYDFVEVAIGPFPTPTPNLTVLPTSTPTITPTHTPGPLRIDL
                     YGGKPIDTMLRGQNPRRGDGTRLNAVAISRNGCVRGVAGGLDADTYTWTDLTTGVDAR
                     NPNVSTLDFLREARDVNAQPLLTANVVGPGYRDATGTWFCQSPSDGWLEKLAADWVLY
                     TNVILQNYRQGDTLPSQYQDLLDSFNWSGKEKLLSATEPLTPKV"
     gene            1718..2242
                     /locus_tag="PKH07_05900"
     CDS             1718..2242
                     /locus_tag="PKH07_05900"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNT34518.1"
                     /translation="MPKETIDDIMQRRSCRHFTGEPLMPDDLNLLMDVVRWAPSAGNR
                     QPWFFYVVLNDQIKHLLAEAALGQGFVAQAPVVFVACVEPELSATRYGDRGRTLYAIQ
                     DVSAAVENLLIAATGLGYGSCWVGAFNEDRARAALHIPGYRRPVAMIPIGRGTLSPER
                     TDRRPVDKVFEMVP"
     gene            2323..2760
                     /locus_tag="PKH07_05905"
     CDS             2323..2760
                     /locus_tag="PKH07_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014452575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate resistance protein"
                     /protein_id="HNT34519.1"
                     /translation="MKWITRAHVHVDRVACPWLITRFIDSDAEFVFVSTGQVQDLAAR
                     EKAIAFDSPNAVLGHRNDKCTFETLIETYGITDRALQRMAKIVHAADMDEPEKDPVAA
                     GLQAIAVGYSLRYPDDWENLEHQFEVYDALYAWCRLMTARETA"
     gene            2831..>3349
                     /locus_tag="PKH07_05910"
     CDS             2831..>3349
                     /locus_tag="PKH07_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNT34520.1"
                     /translation="MANNRRLESLVEFLGLRRSIVGLLGMIVLVGMGERMAERFLPVY
                     LVALGGGEQLVGMLNAMDNLLSALYSFPGGYLSDRFGIKRSLLLFNLLAMLGFLIVIV
                     IPAWPAVFVGSVFFLSWTAISLPATMDLVAKALPKSKRTMGVSMHSLLRRVPMALGPM
                     IGGAFMVRWGQLT"
BASE COUNT          714 a          847 c          952 g          836 t
ORIGIN      
        1 cactttcggg gtcaggggct ccgtggccga cagtaacttt tccttacccg accaattgaa
       61 actgtccaga agatcctggt actgcgatgg caatgtgtcg ccttgccgat agttctgaag
      121 gatgacgttt gtgtagagca cccagtcggc cgccagtttc tccagccagc catcagaggg
      181 gctctggcag aaccatgttc ccgtcgcgtc gcgatatcca ggcccgacca cgtttgctgt
      241 gagcagcggc tgggcgttca cgtcccgtgc ttcgcgaaga aagtccaagg tgctgacgtt
      301 gggattcctg gcatcaacgc cagttgtcaa atctgtccac gtgtaggtgt cggcgtcgag
      361 tcctcctgcg acgcctcgca cacaaccgtt tctggagatc gctacggcgt tcagtcttgt
      421 tccgtcccca cgtcgtggat tctggcctcg aagcatggtg tcaatgggtt ttccaccgta
      481 gagatcaata cggagagggc caggggtgtg ggttggcgtg atggtcggtg tacttgttgg
      541 aagaaccgta aggtttgggg tgggtgtcgg aaaggggccg atggcaacct cgacgaaatc
      601 gtaccagaag gtgccgtttg gtccatattc actgcctcgc ggaaacgtgg ttccaggaac
      661 gttcatcggc acgccgattt caaccgcctg aaagttgtcc atcgttccat tcggcggacc
      721 ggcaaatgtc tttacgagga cgccgttctt gtaaagcttt gcgtcaacat ctgtgcacag
      781 aatctgccag acgatcatct tgtccttgtc cgcggtttcc tggtagagat ctttgactgc
      841 atagacgctt ggaacagctc caccgcagtt ctcacgatcc cgcaggcgat agtccacatt
      901 cgtggcagta ttggggcaga agtaaagcat cggcgaacaa cccgtgaaga gattgtcata
      961 gctcttgagt gctgcgccct tctcagtcct gctgcctgtc atgcgcatat agagtgtaag
     1021 acgaaacggt gctgcggttg tcggatccac tgccgaccag gtaccacgca cacagccgac
     1081 gctgctgcca gcagttgact ttaccgtctt gccacccatg gttgcatttt ccgtggtcac
     1141 actcaggccg ttctcaacca cagaccaacc cgcagtcagg gaagaggcgg tcgtgagagc
     1201 acctgttggc catgtcgaaa agtcttcctg caggatgact gtttctgccg aagctgaacc
     1261 cacaacaaat accaaagcga gtaaagaata aatcagaaga tttctcatcg cagatacccc
     1321 tccatctcga gcaggccagt gcctgcaggc ttgtgtgcta tgcccactcc cttggactct
     1381 tgttcggcaa atgacacgct tcttcagatc tgagtgccat caggagaatc acaaacgcac
     1441 aaggctcaaa aagtacgatt ccaagcctct tcacatcttc tcataccaat gtaggtatgt
     1501 ttccatgcaa ggcaaacagt aatctgtcgc aatccgcttg tcaataagta tagcgtcttt
     1561 ggccagagac aagtcatgtg ctccttgacc gaatcgggaa gaaaaagata cagtatgcac
     1621 gagattacgg aatggatact ctcaatctct tctggaacga agtatcgtca gcctgagaga
     1681 tttggggaga gacaatcctt gatgaaaggt ttgaagcatg cccaaagaaa ctattgatga
     1741 catcatgcag aggcgctcgt gtagacactt tactggcgag cctttgatgc ccgacgatct
     1801 gaatcttctt atggacgttg ttcgttgggc accctcggca ggtaaccgcc aaccttggtt
     1861 cttctatgtg gttctcaacg atcagattaa gcacttgctg gccgaggctg ccttggggca
     1921 gggctttgtc gctcaggctc cggttgtgtt tgtcgcctgt gtagagccgg agctttcggc
     1981 gacccgatat ggcgatcggg ggagaacgct ttacgccatt caggatgttt ctgccgccgt
     2041 ggagaatctg ctgatcgctg cgaccggtct cggatatggg agttgctggg tgggtgcctt
     2101 caatgaggat agggctcgcg cggcgctgca cattcctggt taccggagac ctgttgcaat
     2161 gatccctatt ggcagaggga ctctcagccc agagagaacg gatcgccgac cagtggacaa
     2221 agttttcgaa atggttccat agatcgagat tgtttaggag actctccaga cccataattg
     2281 ggatttgtgg gagttcaaat ctgacgcaca gtggaggttg ccatgaagtg gatcactcga
     2341 gcgcatgtgc atgtggaccg tgtagcctgt ccatggctga tcacgcggtt cattgactcg
     2401 gatgcggaat ttgtcttcgt ttccaccggt caggttcagg acctggccgc tcgggagaaa
     2461 gccattgcat ttgactcgcc caatgccgtt ttgggtcatc gaaatgataa atgcacattt
     2521 gaaacgctga tcgaaaccta tggcatcaca gacagagcgc tccagcgaat ggcgaagata
     2581 gtacacgcgg ctgatatgga tgaaccggag aaggatccag tcgccgcggg tctgcaggcc
     2641 attgcggtcg gttacagcct cagatatcct gacgactggg agaatctgga gcatcaattt
     2701 gaggtctacg atgccctcta tgcgtggtgt cgtcttatga cagcacgcga gacggcttag
     2761 tggcagggca cattggggat tcggacatcg gcagatttgc tgagtgtcta cagcctttgg
     2821 gtgtgttttg gtggcgaaca acagacgtct tgaatcattg gtggaattcc tcggtctccg
     2881 acggagtatc gttgggcttt tggggatgat tgttcttgtc gggatgggtg agcgcatggc
     2941 agagcggttt ctgccggttt accttgtggc attgggtggg ggagaacaac tggtcggcat
     3001 gctcaacgcg atggacaatc tcctttccgc gctgtactcg tttccagggg ggtacctttc
     3061 tgatcggttt ggaataaagc gatcactctt gctgttcaat ctactagcca tgctgggttt
     3121 ccttatcgtg attgtgatcc ccgcctggcc tgccgtcttt gtcggctcag tcttttttct
     3181 ttcatggacg gcaatctccc tgccggcaac gatggacttg gtcgcgaagg cgcttccaaa
     3241 aagcaaacga acgatgggag tttcgatgca ctctttgctt cgtcgagtcc cgatggcact
     3301 tgggccgatg atcggtggcg cgttcatggt tcgatggggg cagttgacc
//
LOCUS       DAOQUC010000272         3348 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_32584_length_3348_cov_2.891588, whole genome shotgun sequence.
ACCESSION   DAOQUC010000272 DAOQUC010000000
VERSION     DAOQUC010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3348)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3348)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3348
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32584_length_3348_cov_2.891588"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..783)
                     /locus_tag="PKH07_05915"
     CDS             complement(<1..783)
                     /locus_tag="PKH07_05915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34521.1"
                     /translation="MPLLPTVGRKALRVRLLILSIYGVLVLGGITMVIPFLITLTCSV
                     SNRFDLEKYNPVPTYFWEDDELYCKYLFEKYEFNLLRRLHRLVHLTTEREMRMAKDVV
                     GDFFPAVQHIRSGIGCPNIPLIRSDYESFLMRYPSERLCGLFPNMLEEKYRRWLKQKY
                     TSQLGQEALAKSAQKNEELALQLFNRDNGEIVATEFALIEFNLDLSFSPKAFEEDQLR
                     FREQRLFLAELEPQWKRPEMLDEFFERYAKRKYTTIANVNAAW"
     gene            complement(785..>1560)
                     /locus_tag="PKH07_05920"
     CDS             complement(785..>1560)
                     /locus_tag="PKH07_05920"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNT34522.1"
                     /translation="KLVGRSEWVGIDNFITVALSPDFYRYLFITFKFVFLHLSMGFIA
                     PIVLALLLNEVPKGKLFFRMVFMLPRVSVPLVITFLWKTMYHPTEAGFFNSLLLRTGL
                     ITQPLKFYDDPNIALICCIIPQVWASMGIGSLIYLAALKSVPEELYEAAEMDGASIMR
                     RLFNITLPTLKPLILINFIGACINTFHSMGNIFVMTGGGPNGATTVLSLAIWRHAFVN
                     LDFGMATATAWILGTMLIGFTIYQLKFLQKVEFRRAEVN"
     assembly_gap    1561..1570
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1571..>3348)
                     /locus_tag="PKH07_05925"
     CDS             complement(<1571..>3348)
                     /locus_tag="PKH07_05925"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNT34523.1"
                     /translation="PRYPSTRAYNEYQALNPNIKIIPFEPLRLQGALAGTDSSVLMSF
                     AGKTAPDVVMTQFHKLRAFARNNFIMDLSEFIGEDTDGDGYISDEEALWEPWKQLRPQ
                     VRCAGTIDGKPYGVPKGDFLLGLCYRRDVLESLFEKSKVKRVPENFDELFYLCQKATS
                     PQIEIRGAKYNFGRRGIHLSTYGWDWYPWLWAAGGDCVQQGKYNPKTDKWHWYKKEEL
                     DFLDPETGESLHRQPTRWKATFASEAGMEAMELYWRFCHQPWILDDQIDSETQDREPI
                     NLTDADVRRGSITLPDGRIVRFEPRDVRIGVARGYQGGDDVNAQEVFGNGEVVFTYAM
                     PLYFLASCLERGLTAQQIGFMPLPKGMGPKATPAFFWENQWLGINYLLKDDPVKKKIV
                     FDIISHSARRFTEFQVANAVESNLAEVMRPDLLEQHGYFEYVDQVPKHLADAFAMVEQ
                     YGRTEPYEGFWQPVQDVILGGLVEEVFASASYDWRKGLVEAENKANTEYLSKRPEEKM
                     QQLRRTGYPIFAAFLALFVGMFLYFLRVMRQKYLSGMDASALRQKHRSSGVFGKVIPI
                     VMIGPALCLIAIWSYYPMLKGSLMSF"
BASE COUNT          822 a          842 c          867 g          807 t
ORIGIN      
        1 ccaagcagca ttcacgttgg caatggtcgt atacttgcgt ttcgcatagc gttcaaaaaa
       61 ctcatcgagc atttccggcc gcttccattg aggttctaac tccgccagaa agagtcgctg
      121 ttcgcgaaag cgcagttgat cctcctcgaa tgctttagga ctgaagctta agtccaggtt
      181 gaactcaatg agtgcgaact cggtagcgac gatctcccca ttgtctcggt tgaacaattg
      241 cagtgccaac tcctcattct tttgggcgct cttggcaagt gcttcctgtc caagttggct
      301 ggtgtatttc tgcttcagcc atcggcggta cttttcttcc aacatgttgg ggaagaggcc
      361 acagagacgc tcagagggat acctcatgag gaaagactca tagtcggatc ggattagagg
      421 aatgttgggg cagccgatac cactacggat gtgctgcacg gcagggaaga agtctccgac
      481 cacatccttt gccatcctca tctccctctc cgtggtcagg tggacgagcc tgtgcagacg
      541 ccgcagaagg ttgaactcat atttttcgaa aaggtacttg cagtagagct catcgtcttc
      601 ccagaagtag gtggggactg gattgtattt ttcaagatcg aaccgattgg acacgctgca
      661 tgtgagggtg atcagaaacg ggatcaccat cgtgattcca cccagcacaa gaacgccgta
      721 gatggaaaga atcaagagtc ttacgcgaag ggctttgcgt ccaactgttg gcagtagagg
      781 catgtcagtt gacctccgcg cgtcgaaatt ccaccttctg caggaatttc agttggtaga
      841 tcgtgaatcc gatcagcatc gttccaagaa tccaggcggt agctgttgcc atgccaaagt
      901 caagattcac aaaggcatgt cgccatattg caagagataa gaccgtggtg gcgccattgg
      961 ggccgcctcc ggtcatgaca aagatgtttc ccatggagtg gaaagtgttg atacaagcgc
     1021 cgatgaagtt gatcagtatc agtggcttca gagttggcag agtgatattg aaaagacgac
     1081 gcatgatact ggcaccgtcc atctcagctg cctcatagag ttcctcggga acgctcttca
     1141 acgcggcgag atagataagg ctaccaatcc ccatacttgc ccaaacttgt gggatgatgc
     1201 agcatatcaa ggcgatattc ggatcgtcgt aaaacttaag tggctgtgtg atcagtcctg
     1261 tgcggagcaa tagtgagttg aaaaagcctg cttctgttgg atggtacatg gtcttccaaa
     1321 gaaatgtgat gacgagaggc acagagaccc gcgggagcat gaagaccatt cggaagaaga
     1381 gtttgccctt gggaacttcg tttaggagca acgccagaac gataggggca atgaacccca
     1441 tggaaagatg caaaaagacg aacttgaaag tgatgaacag atatctgtag aagtcaggac
     1501 tgagggcaac ggtgataaaa ttgtcaattc ccacccattc gctcctgcca acgagcttgt
     1561 nnnnnnnnnn gaaaactcat caaagaacct ttgagcattg gatagtacga ccagatggca
     1621 atgaggcaga gagcgggacc tatcatgaca attggaatga ctttgccaaa gactccactg
     1681 gagcggtgct tctgtctcag tgcggacgca tccattccag agagatactt ctggcgcata
     1741 accctgagga aataaaggaa catgccgaca aagagagcca gaaacgccgc gaagattgga
     1801 tatcctgttc ggcgaagttg ctgcattttc tcttcaggac gcttcgagag atactccgtg
     1861 ttggctttgt tctctgcctc aacgagacct ttgcgccagt cgtacgatgc cgaggcgaaa
     1921 acctcttcca cgagtccgcc gaggatcaca tcctgcacgg gttgccagaa tccttcatag
     1981 ggctctgtgc gaccatattg ttccaccatg gcaaacgcgt cggccaggtg ctttggcact
     2041 tgatctacat actcaaagta gccatgctgc tcgagcagat ccggtctcat cacctctgcc
     2101 agattgctct caactgcatt ggctacctga aactcggtaa aacgtcttgc tgagtgtgag
     2161 atgatgtcaa acacgatctt cttcttgacg ggatcgtctt tcagaagata gttgatcccc
     2221 aaccactgat tttcccagaa gaaagcaggg gttgcttttg gtcccattcc tttgggcaaa
     2281 ggcataaaac cgatttgctg tgccgtcagc cctcgttcga ggcagctggc caggaaatag
     2341 agaggcattg cataggtgaa gacaacctca ccattaccga agacttcctg tgcattcaca
     2401 tcgtcgccac cctggtatcc acgggcaaca ccaatgcgaa catccctcgg ctcgaatctc
     2461 acgattctcc cgtcaggaag ggtgatagac cctcgacgca cgtccgcgtc ggtcaagttg
     2521 atcggttcgc gatcttgagt ctcgctatca atctggtcat cgagaatcca cggctgatgg
     2581 cagaatcgcc aatacaactc catcgcttcc atacctgctt ctgaggcgaa ggttgctttc
     2641 cagcgagttg gctggcgatg aaggctttct cccgtctcgg gatccaggaa atccagttct
     2701 tctttcttgt accaatgcca cttgtctgtt tttggattgt acttgccctg ctggacgcag
     2761 tccccaccgg cagcccaaag ccacggatac cagtcccacc cataggtgga gaggtgtatt
     2821 ccacgcctac cgaaattgta ctttgcacct cgtatctcga tctgcggcga tgttgccttt
     2881 tgacagaggt agaagagttc gtcgaagttc tcaggaactc ttttgacctt ggacttttcg
     2941 aatagtgatt ccaggacatc acggcgatag caaagcccga gcaggaaatc tcccttgggg
     3001 acgccatagg gcttgccgtc tattgttcca gcacatcgaa cctgtggacg caactgcttc
     3061 cacggctccc agagggcttc ttcatctgaa atgtagccgt cgccgtcggt atcctctcct
     3121 atgaattccg agagatccat gatgaagttg ttgcgagcga aggcgcggag tttgtgaaat
     3181 tgagtcatca ctacatcggg agcagtcttg cccgcgaaac tcatgagaac actgctgtcg
     3241 gttccggcca gagccccttg cagtcgaagc ggttcgaaag ggataatctt gatattggga
     3301 ttgagtgcct gatactcgtt ataggctcgg gtagaaggat accttggc
//
LOCUS       DAOQUC010000273         3347 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_32594_length_3347_cov_3.537363, whole genome shotgun sequence.
ACCESSION   DAOQUC010000273 DAOQUC010000000
VERSION     DAOQUC010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3347
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32594_length_3347_cov_3.537363"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..507)
                     /locus_tag="PKH07_05930"
     CDS             complement(<1..507)
                     /locus_tag="PKH07_05930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34524.1"
                     /translation="MSGQKKSSIIRIAVAITCLFALTGCGLWKKYTANSQIKKVEKKL
                     EEARQKESPKYTSDLYSEAENLVTEAKSLVEQKQYNDAIQKANEAEGRVNMSIEQVDV
                     AKTRIAREIKEVRDAIEEVRVIVEKAEQSGGEQIAPDLLSKAKAGYSSLVTARDQQEQ
                     NTVQKTADY"
     gene            complement(584..964)
                     /locus_tag="PKH07_05935"
     CDS             complement(584..964)
                     /locus_tag="PKH07_05935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34525.1"
                     /translation="MAAPTRTENEIEEVRNFIENWILYMKMFKKAHYGGEITREDEQN
                     FLKLKSLVARKHQYLMDSLGSDYIGAQPVTKILSLCVNLQIITRVNRSHYVDVETKWH
                     DTFLALNETMGHLRYRLQKGGKGK"
     gene            complement(1109..1987)
                     /locus_tag="PKH07_05940"
     CDS             complement(1109..1987)
                     /locus_tag="PKH07_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34526.1"
                     /translation="MNRKNSIARVLIPVFWLAVLIVSFVFIRASLAQEEAFRQYDPDP
                     ETMMNEESRTLNRTRGEMNLRGEGMPDAQPTDQPPTIMVKTGERVFCAMCGRKLDDTV
                     HFEEVLLEQAGGYYDDGSHNDEVPRDGLPSNIIEVRTSYIGPYCGLLRDRLLSYWVKV
                     KNPQTWGYYRSASYPRHWAAVAFYGLPVAALDRVKAESPLEPLTRWSQQLEEYVAFWH
                     SFYVQPYFQYEIHPDNLAGPQMMRRGPMMWGMESPEMMMMMQEEGFYGPEDFNPQSHP
                     TADKINRANRAADMMK"
     assembly_gap    2091..2100
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2399..2968)
                     /locus_tag="PKH07_05945"
     CDS             complement(2399..2968)
                     /locus_tag="PKH07_05945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34527.1"
                     /translation="MKGTCALGLVCVALLFVAAGSWAEEKACCPSEKPCPIVSAEADP
                     APVPVAVTPERGMRARGEQGDLIGTVLGKLNLTEEQKAQIKPIREKQMEEMKTLRADT
                     TVPREEMWQKARASNEKYRAEIMGILTEEQKAEAEKLMAEARQKAREGQRPEMRNRQG
                     RREGEGQPKAEGQGAGQRRGRGGEGKKAE"
BASE COUNT          760 a          969 c          736 g          872 t
ORIGIN      
        1 gtagtctgct gttttctgaa cagtattctg ctcctgttgg tcgcgtgccg ttaccaagga
       61 ggaatatccc gccttggcct tgctgagaag atcgggagcg atttgttccc caccagactg
      121 ctccgccttc tccacgatga cgcgaacctc ttcaatggca tcccgaactt ctttgatctc
      181 tctggcaatt cgggtcttgg caacatccac ctgctcgatg gacatgttga ctctgccctc
      241 ggcctcattt gccttctgaa tggcatcgtt gtactgcttc tgttccacaa ggctcttggc
      301 ctctgtcacc aggttttcag cctcggaata caggtctgac gtgtacttgg gggactcctt
      361 ctggcgggct tcctccagtt tcttctcaac cttcttgatt tggctgttgg ctgtgtactt
      421 tttccaaagt ccacatcctg tcaacgcgaa caaacaggta atcgccacgg cgatccggat
      481 gatgctcgat ttcttttgac cgctcattcg gaattcctcc aagaattgac cgttatcttt
      541 atctttacag agaagccccc tgcggggggc tcggattcaa tgcttacttt ccctttccac
      601 ccttctgcaa acgatagcgc agatgtccca tggtctcgtt caaagccaga aacgtgtcat
      661 gccacttggt ctcaacatcc acataatggc tccggttgac acgggtgata atctgaagat
      721 tcacacacaa ggaaagaatc ttcgtgacgg gttgcgcacc aatataatca cttcccaaac
      781 tgtccatcaa gtattgatgt ttgcgggcga ccagagactt cagtttcagg aagttctgct
      841 catcttctcg cgtgatctcc ccgccatagt gcgctttctt gaacatcttc atatagagaa
      901 tccagttctc aatgaagttc cgaacttcct caatttcatt ttccgtccgc gttggtgctg
      961 ccatattctg tctgacccac cccgttggtc ttgctttatc tataccagaa agcgaccgct
     1021 ctgttcacag aaaccagact tatccatttt cccgcttcac ttgaaaatca ggaactccaa
     1081 gacgggagaa gtctcccgct ccagttgcct atttcatcat atccgcagcc ctgttggctc
     1141 ggttgatctt atccgctgtt ggatgactct gcgggttgaa atcctctgga ccgtaaaatc
     1201 cctcttcctg catcatcatc atcatctccg gagactccat accccacatc atcgggcctc
     1261 ttctcatcat ctgggggcca gcaagattgt caggatgtat ctcgtactga aaatagggct
     1321 gaacatagaa actgtgccag aatgcaacgt actcctccaa ctgctgagac caccgagtaa
     1381 gaggctcaag gggactctca gccttcactc gatcgagcgc tgcaaccggc aagccataga
     1441 acgccacagc agcccagtgt cgaggatagg acgcactccg ataatagccc caagtctgag
     1501 gattcttaac cttcacccag taactcaata gccgatcgcg tagaagacca caatacggtc
     1561 caatgtagga tgtgcggacc tcgataatgt tcgagggaag gccgtcgcga ggaacctcat
     1621 cgttgtgaga accgtcatcg tagtaaccac ctgcctgctc cagaagaacc tcttcaaagt
     1681 gaaccgtgtc atcaagcttt cggccgcaca tggcacagaa aacccgttcg ccggtcttca
     1741 ccataatcgt cggaggttgg tcggttggtt gggcgtcagg cattccctct ccacgaaggt
     1801 tcatctcgcc tcgcgttcta ttcagagttc gactctcttc gttcatcatg gtctccgggt
     1861 cgggatcata ttgtcgaaaa gcctcttcct gggcgagaga cgcacggatg aacacaaaag
     1921 acacaatgag gacagctaac caaaagacag gaatcaaaac acgagcaatg ctgttttttc
     1981 ggttcattgg ccgactcctt tccctggcgc tcccttaagg aaacagccca agagaaggat
     2041 acacctttta gacttacaac gcaaggctca aacccttctt ggaacacacc nnnnnnnnnn
     2101 ttccaagacc cggattcggc actttagcca agaatatcgt gtcgctggtc tgatgtcaat
     2161 caaaaattga atttaacaga acttaagtac gttagcttca gagcgagtat agggctatcc
     2221 aatccaactc aagacgattc catgactcaa ctgagttccg tttccagaga cctcttgagt
     2281 cgttggcagc atttcaagtc tgggtttcgg atttacctcc aaacgataaa aggcaagagg
     2341 aggaagcaaa aggccttcct cctcctcatc cgtattgttg tgaaatctcg gaacgggctt
     2401 attcagcctt tttcccttct ccacctcggc cacggcgctg accggcaccc tggccttcag
     2461 ccttcggctg gccttctcct tctcggcgac cctgacggtt acgcatttca ggtctctgac
     2521 cttcgcgtgc cttctgacgg gcttcagcca tcagcttctc ggcctcagcc ttctgctctt
     2581 ctgtcagaat ccccatgatc tcggcgcgat acttttcgtt tgatgctctt gccttctgcc
     2641 acatttcctc acgaggaacc gtggtatcgg cgcgaagagt cttcatctct tccatctgct
     2701 tctcacgaat cggtttgatc tgtgccttct gctcttcagt caagttcaac ttaccgagca
     2761 ccgttccaat caaatccccc tgctcgcctc tcgctctcat acctctctcg ggcgtcaccg
     2821 caaccggaac gggagcagga tccgcctctg cactgacgat cggacagggc ttttctgatg
     2881 gacaacaggc tttctcttct gcccagcttc ctgctgcgac gaacagcaac gcgacgcaaa
     2941 ccagacccaa cgcacatgta cctttcattc ggaactcctc cttaattctt gctttccgat
     3001 tctcttgccg gaaagtcttt cttcgcaatt catctaacaa aacgaagcag gacttgtgcc
     3061 aataacggaa tgcggtcttc cttggagttc ggtatccctt caacagtttg aaacctaaag
     3121 agttgcaaac gagccgtacc atgcgttgca cccgtggcaa ctcggagacc gattgtggat
     3181 attccccttt gcgtcgcgaa aatcgcacca tatcgtacac ttggactcga gaagagcata
     3241 cggatggcat ttgagcaggc agccgcctga aggcagatat ctggcttcct cgatagccga
     3301 gagcaagaac agatactgtc aagaaagaga cctgtgctag aatcggc
//
LOCUS       DAOQUC010000274         3342 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_32654_length_3342_cov_3.263158, whole genome shotgun sequence.
ACCESSION   DAOQUC010000274 DAOQUC010000000
VERSION     DAOQUC010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3342
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32654_length_3342_cov_3.263158"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1495
                     /locus_tag="PKH07_05950"
     CDS             <1..1495
                     /locus_tag="PKH07_05950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34528.1"
                     /translation="YTWPPSSSLSGVVDNSDAGFTVVSGTWTTGTSATDKYGADYRYK
                     TTGTGSAVVRWTPSLANTGNYDVYVWYPQGTNRPNNAPYTVNFTGGSQAFLVDQTTNG
                     GKWNLLGTFYFQSGTGSIELSDNAQASKAVMADAVRFAWSATQPTATPTRTPTQTPTY
                     TPVAGIFVDDGDSGYSSGGTWSYVSAGGTQPYNGDYYWADSNPSSESAWAKWTPNLPA
                     RRIYEVYVMFRSGSNRPTNAPYTVYYSGGSQTVTVNQTINGGVWVLLGSYIFDAGTAG
                     YVRLSNYPIDAGKAVIADAVRWLDVGAAPTNTPTNTPTRTPTNTPTRTPTNTPTNTPT
                     RTPTNTPTRTPTNTPTNTPTPAVTPVERILDDGDSGYTKSSGWTYVSAGGTEPLNGDY
                     DWASTVTGSPTRWAKWTPNLTAAGNYSVYVRFRMGSNRANNAPYTVVYNGGTQTIAVN
                     QTVGGGTWVLLGTFNFASGTAGYVRLDNGPAQGSKVVIADAVKFTPQ"
     gene            complement(1852..2184)
                     /locus_tag="PKH07_05955"
     CDS             complement(1852..2184)
                     /locus_tag="PKH07_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNT34529.1"
                     /translation="MRTRGFTLVEIMIVVAIIGLLAAIAIPNFIQARKDSQANSCSQN
                     LEQIAGAKEQWAFANNAPTTASPGTADLADYLSDSFTWECPAGGTYSINNMATNPTCS
                     IGDPHMIR"
     gene            complement(2567..>3342)
                     /locus_tag="PKH07_05960"
     CDS             complement(2567..>3342)
                     /locus_tag="PKH07_05960"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF015647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HNT34530.1"
                     /translation="TGLPVIADFRVADMAAGGNGAPLVPFADFHLFSRPEESVVIQNI
                     GGIANCTLIPASGRIEDIVAFDTGPGNMIIDGLTRHFYPDERFDHNGVHAQRGKPIEQ
                     VLNHWMAMPYIRSHPPKTTGRELFGEPFVREVIRDYPETAPDDLISTATWFTAASIAF
                     SLCEFIMPVWQFSDLILAGGGAENAFLVQALGSEMQRRMVNRSVRILKSDEVGHPSKP
                     RECVAFALLGHARLRGIPSNVPSATGAREAVLLGKIIEP"
BASE COUNT          802 a          915 c          879 g          746 t
ORIGIN      
        1 atacacttgg ccaccgagtt cgtctctttc gggtgtggta gacaactcag atgccggctt
       61 cactgtcgtt tcgggtacat ggactacagg gacttctgca acagacaagt acggagcgga
      121 ctaccgatac aagaccaccg gaaccggctc tgcagtcgtc agatggacac ccagtcttgc
      181 gaatacgggc aactacgacg tgtacgtctg gtatccccaa ggaaccaacc gccccaacaa
      241 cgcgccctac acggtcaact ttacaggtgg ttctcaggcg ttcctggtgg atcagaccac
      301 caacggtggc aagtggaatc tgctcggaac tttctatttc cagtctggaa caggttccat
      361 cgagttgagc gacaatgccc aggcaagcaa ggcagttatg gcagacgctg ttcgatttgc
      421 ctggagtgcg actcaaccga ccgcaacccc gactcgaaca cccactcaaa cgccaacata
      481 cacgccagtt gccggcattt ttgtggatga tggggactcg ggctactcaa gtggcggaac
      541 gtggtcatac gtgagcgctg gtggaacaca accttacaat ggagattact actgggcgga
      601 ttccaacccc tcctcagaat ccgcttgggc aaagtggaca ccgaatctgc ccgccaggag
      661 aatctatgaa gtctacgtga tgttccgatc gggcagcaat cgtccgacca atgctcctta
      721 cacggtgtac tacagcgggg gttcgcagac ggtcacagtg aaccagacga tcaacggtgg
      781 agtctgggtg ctcctgggga gctacatttt cgatgccgga acggctggtt atgtccggct
      841 ctccaactac ccgattgatg ccggtaaagc ggttattgct gacgcggttc gttggttgga
      901 cgtaggcgca gcacccacga acactccgac gaacacaccc acgcgaacgc cgacgaatac
      961 accgacgcgg acacccacaa atacgccaac gaacacgcct acaagaacac cgaccaatac
     1021 tccgactcga acgccaacga acacaccaac gaacacccca acaccggcag tcactccggt
     1081 ggaacggatt cttgacgatg gtgactccgg ttacacgaaa tccagtggtt ggacctatgt
     1141 ttctgccggt ggcaccgaac cactcaatgg cgactatgac tgggcgagca ccgttacggg
     1201 ctctccgaca cgatgggcga aatggacacc gaacctgacc gcagcgggca attacagtgt
     1261 ctatgtgcgt ttccgaatgg gcagcaaccg tgccaacaat gcaccatata cggttgtcta
     1321 caatggcggt acgcagacca tcgccgtcaa ccagacggtt ggcggaggaa cctgggtgct
     1381 gctgggaacc tttaactttg catcgggaac agctggttac gtccggctgg ataacggccc
     1441 agcgcaggga agcaaggtgg tcatcgctga cgcggtgaag ttcacccctc agtagacggc
     1501 tactgcagtg aaggatgtca ccgcctggtg gcatcctcga gcccaaccaa agtggctcag
     1561 gggcggaagg gtcgaatcag attcttccgc ccttgatctt ttttacgccc tttgggagac
     1621 atgagtcaga aggtgttgag ttctcgctca ttggtgctgt gcagtctgta caccgcccag
     1681 tttcgtacag gttccgaacg actcacagga tagggggccg ctttcagtgc tgaggtttgg
     1741 gtggagtgcc gtgttacaaa gactgaggga ctaccgagcg atttccatat aaacaagcaa
     1801 ggcggcgaac cataggtccg ccgccttcca agcaatcatg gtcacggtaa tctaacgaat
     1861 catatgcggg tcgccaatgc tgcaggtcgg attcgtggcc atgttgttga tgctgtacgt
     1921 tccaccagca ggacattccc aagtgaaact gtccgaaagg taatcggcca agtctgctgt
     1981 tcctggggaa gccgttgtgg gtgcattgtt cgcaaatgcc cactgctctt ttgctccagc
     2041 gatctgctcc aaattctggg agcaggagtt ggcctgtgaa tctttacgag cctggatgaa
     2101 gttcgggatc gcaattgcag ccaacaaccc gatgatcgct acgacgatca tgatctcaac
     2161 caacgtgaaa cctcttgtcc tcataataac cgcctcctat tgatttttgc tgtctcagtg
     2221 actagctttg ttcatgtatt agagcaggtg ttgtgccaat ttcgttgccg accacgatcc
     2281 acctctcgat ccaaccgtcc atgagttgat taactcattt aatataaaca ggttagactg
     2341 aacatatggc tctactgtca gaatgtcatt tgtgcggata ttcaagcgaa ttcgaggcgt
     2401 gccatgagac ttatcgttgt atttctgtcc ttttatggga catctatgtc acacaatccg
     2461 tgaatctccg ccctcgtcac cttaggttcc atatttgtcg ggacaagagc agcccaacct
     2521 tctgaatgtc tgtcgaaagg cactgaatct gccacctgtg gcaggctcac ggctcgatga
     2581 tctttccaag caagaccgcc tctcgggcac ctgttgcgga ggggacgttg gaaggaattc
     2641 cacgaaggcg agcatgaccc agaagcgcga atgcaacaca ctcacgtggt ttggaagggt
     2701 ggccgacttc atcgctcttg agaattctga ctgagcgatt gaccatcctc ctctgcatct
     2761 cgcttcccag agcctgaaca aggaatgcgt tctccgctcc accacctgca agaatcagat
     2821 cagagaactg ccagacgggc atgatgaact cgcagagact gaaagcaatt gaggcagcgg
     2881 tgaaccaggt tgccgtggaa atgaggtcat caggtgcagt ttccggatag tcccgaataa
     2941 cttctcggac aaacggttct ccaaacagtt ctctccccgt cgtcttgggt ggatgacttc
     3001 tgatgtacgg catcgccatc caatgattca ggacttgttc gatcggtttc ccacgttgag
     3061 catggactcc attgtgatca aaacgttcat caggatagaa atggcgtgtc agaccgtcaa
     3121 tgatcatgtt ccctggtcct gtgtcaaaag ccacaatatc ttcaatgcgt cctgaggcag
     3181 gaatcagagt gcagttggcg attccaccaa tgttctgaat gacaacactt tcttcggggc
     3241 gtgagaacag atgaaagtca gcaaatggca caagcggtgc gccgttgcct cccgctgcca
     3301 tgtccgccac tcgaaaatca gcgatgacgg gcagccctgt tc
//
LOCUS       DAOQUC010000275         3338 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_32697_length_3338_cov_3.261042, whole genome shotgun sequence.
ACCESSION   DAOQUC010000275 DAOQUC010000000
VERSION     DAOQUC010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3338
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32697_length_3338_cov_3.261042"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="PKH07_05965"
     CDS             complement(<1..270)
                     /locus_tag="PKH07_05965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34531.1"
                     /translation="MLFRKKTKDKEKEKSQGDFSTVGLDIFSEESPSSEPPRQPETPK
                     QTGGSVSSPPLKTKAGAPGREPVADIPALDDALVLPEDTELAQSLP"
     gene            complement(405..1466)
                     /locus_tag="PKH07_05970"
     CDS             complement(405..1466)
                     /locus_tag="PKH07_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014436962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HNT34532.1"
                     /translation="MLDINDLLKMVVDRNASDLHLRVGRPPTIRIDGRLENLGTEPLT
                     AEETQSLMESIAPDRHREAIAETGSSDFGYTFENLARFRVSIFRTMGNVGIVLRLIPH
                     RILPFEALRLPRNAIIDVLHRPRGLVLIVGPTGSGKTTSLATMIDYINANRDCHIITI
                     EDPIEYFHPHKRSIVTHIELHVDTPSFARALRHALRQDPDVILVGEMRDLETMEAAIT
                     AAETGHLVFATLHTNSAAQTINRIVDAFPVTQQEQIRMQLSVTLVAVISQQLLGHASG
                     KGRLAAYEIMIMTPAIANLIRERKTNRIISSIQTGQKLGMVTMDQFLLGLYNQKLITA
                     EDCIHKSHNADEMRQAILT"
     gene            complement(1519..3261)
                     /locus_tag="PKH07_05975"
     CDS             complement(1519..3261)
                     /locus_tag="PKH07_05975"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HNT34533.1"
                     /translation="MDHKNERILRNALEEAGLVSRERIVAAEQYAQEKGVAFEEGLVQ
                     MGIVEPADILQAVAESVGMKVAHLSECRPEKEVIELISKDQAAKYSAIPIARNGNTVQ
                     VAISDPFDVETMDLLTVLLGELQCRVEFLLAERESIERALERFYTGDEARLEEMFRNI
                     TQSEDIVRISEEMKDESIQSLAASIDESKLEQDDSPIIRLVDKILEQAIRARASDIHF
                     EPFEKIFRVRFRIDGVCQEFEAPPKRLQAAILSRLKLLAGMDLAEKRIPQDGRIQTKV
                     DERAIDFRVNALPGLFGESVVLRILDKGAVLLGLEQVGFAQEQQDIFQKLILKPNGII
                     LITGPTGSGKTTTLYAALNTINTVDRKIITVENPVEYRIEGINQVQINDDIGLTFALS
                     LRTILRQAPNVILVGEIRDQETAEIAIRAALTGHLVFSTLHTNDAPSAATRLTEMGVK
                     PFLIASSVQAVLAQRLVRKICKECKYSYKPDPLVLEDFGLDPAQYEDALLYQGRGCDN
                     CNYSGYKGRTAIFEIMIMSEELQNLILEHRPAEEIRDKAREQGMSLLREDGVIKAFRG
                     ETTLTEVARITGTK"
BASE COUNT          742 a          941 c          863 g          792 t
ORIGIN      
        1 gggaagcgac tgcgccagtt ccgtgtcctc gggaagaaca agcgcatcat caagagcggg
       61 aatatcggca acaggttccc gccctggggc tcccgccttg gtcttgagag ggggagacga
      121 caccgagcca ccagtctgct tcggagtctc gggttgcctg ggtggctcgg aagatgggga
      181 ctcctcactg aatatgtcaa gaccaacggt gctgaagtca ccctggctct tttccttctc
      241 cttgtctttg gtcttctttc ggaaaagcac ctgttagtgt gttcctcgca tttcttcgtt
      301 ctcagccaac aagcaccctc cggcgggcag acgatgccaa ttccaacggg cgcttgcttc
      361 tggcgaaaga cagacttccc gcatttctgt ctgctcgaaa gacctcaagt caaaatcgcc
      421 tggcgcattt cgtccgcgtt gtgggatttg tggatgcagt cttccgcggt gatcagtttc
      481 tggttgtaaa gaccgagaag aaactgatcc atggtcacca ttcccaactt ctgtccagtc
      541 tggatactag agatgattcg gttggtcttg cgttcacgaa tcaggttggc gatagcggga
      601 gtcatgatca tgatctcgta agcggccaag cgtcccttgc cactggcatg acccagaagc
      661 tgctgggaga tgactgcaac caaagtaacg gacaactgca tccgaatctg ttcctgctga
      721 gtgacaggaa acgcatccac aattcggttg attgtctgcg cggcgctgtt ggtatgaagc
      781 gtggcgaaga ccaagtgacc tgtttcggca gccgtaatcg cggcttccat ggtttccaag
      841 tctctcatct cacccacaag gatgacgtcc ggatcctgcc gaagtgcgtg tcgaagagcc
      901 cgggcgaaag acggagtgtc cacgtgcaat tcaatgtgag tcacgatgga tcgcttgtgc
      961 ggatgaaagt actcgatggg gtcttcgata gtgatgatgt ggcagtcgcg attggcattg
     1021 atatagtcaa tcatcgttgc cagggatgtg gtctttcctg aaccggtcgg gccgacaatc
     1081 agcaccagtc ccctgggacg atgaagaaca tcaatgatgg cgtttctggg tagccgaagg
     1141 gcctcaaagg ggagaatgcg gtgcggaatc agacggagaa cgattccgac gttgcccatc
     1201 gttcgaaaga tggacactcg aaaacgcgcc aggttttcga aggtgtaccc gaagtcggaa
     1261 ctccctgttt ccgcgatggc ttcccgatgt cgatcagggg caatgctctc catcaacgac
     1321 tgggtttcct ccgccgtgag tggctctgtc ccgagattct cgagtcggcc gtcaatgcgg
     1381 atcgtcgggg gacgcccaac ccgaagatgc agatcggacg cattgcggtc tacgaccatc
     1441 ttgagaaggt cattgatatc taacacagcg aatcatcctc cgctcggcgg gctccatccc
     1501 catctgggga cgtttcgcct acttcgtgcc cgtgattcgg gcaacttccg tcagtgtcgt
     1561 ctctccgcga aaggccttaa tgacaccatc ttcacgcaga agcgacatgc cctgttcccg
     1621 tgccttatcc cgaatctctt cggcgggacg atgttcgaga atcaagttct gaagttcctc
     1681 agacatgatc atgatctcaa aaatcgccgt tcgccctttg tatccggagt aattgcagtt
     1741 gtcgcaccct cttccctgat agagcaaggc atcttcgtac tgcgccggat ccaaaccgaa
     1801 atcctccagc accagaggat cgggtttgta tgagtacttg cattccttgc atatctttcg
     1861 cacaagacgc tgcgcaagca ccgcctgcac ggatgaggca atcaaaaaag gtttgactcc
     1921 catctccgtc aaacgggtgg ccgcggatgg agcatcgttg gtgtgcagcg tcgagaaaac
     1981 caaatgcccc gtcaaagcgg ctcgaatagc aatctccgcc gtctcctggt cacgaatctc
     2041 tccaaccagg attacgttgg gcgcctgtcg aagaatagta cgcaagctga gcgcaaacgt
     2101 caaaccaata tcgtcattga tctggacttg attgatcccc tcaatacgat actcgacggg
     2161 gttctcaaca gtgatgattt tgcggtcaac cgtgttgatc gtattgagcg cagcatagag
     2221 tgtcgtggtc tttccacttc ccgtcggtcc agtgatgagg ataatcccgt tgggcttgag
     2281 aatgagcttc tggaagatgt cctgctgttc ctgcgcgaat ccaacctgct ccaaaccgag
     2341 caaaaccgcc cctttgtcca gaatacgaag aaccacggat tccccgaaca agccgggcaa
     2401 tgcattgact cgaaagtcta tcgcccgttc atcaaccttg gtctgaattc gaccgtcctg
     2461 ggggatgcgc ttctcggcaa gatccattcc cgccagaagt ttgaggcgag aaagaattgc
     2521 ggcctgaagt cttttcgggg gtgcctcgaa ctcctgacac acaccgtcaa ttcggaatcg
     2581 cacccggaat atcttctcaa aaggctcaaa atgtatgtcg ctggctctgg cacgaatcgc
     2641 ctgttcaaga atcttgtcca caagacgaat aattggagag tcatcctgct ccagtttgct
     2701 ctcgtcaatg gaagccgcga gagactgaat gctctcatct ttcatctctt ccgagatgcg
     2761 gacaatgtcc tcactctgag tgatgtttcg gaacatctcc tcaagacgcg cttcgtcgcc
     2821 tgtgtagaaa cgttcgagcg cacgctcgat ggattcccgt tcggcaagca ggaactcaac
     2881 gcgacactgc agttctccca aaagcaccgt cagcagatcc atcgtctcaa catcaaaggg
     2941 gtctgagatc gccacctgaa cggtgttacc attgcgcgcg atcggaatcg cactgtactt
     3001 ggctgcctgg tctttactga tcagctcaat gacttctttc tccggacggc attccgaaag
     3061 atgagccacc ttcatgccca cgctttctgc aaccgcttga agaatgtctg ctggctcaac
     3121 aatgcccatc tggacgaggc cttcctcgaa ggccacgccc ttttcctgcg catactgttc
     3181 cgctgcaacg attctctccc gcgaaaccag acccgcttct tcaagcgcgt ttcgcaggat
     3241 ccgctcgttt ttgtgatcca ccttcgtcct ccgtatgctc tggctggcac tcacagcacg
     3301 acaggcaacc gaccgatctc aaagatcctg aatctgaa
//
LOCUS       DAOQUC010000276         3336 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_32733_length_3336_cov_3.352027, whole genome shotgun sequence.
ACCESSION   DAOQUC010000276 DAOQUC010000000
VERSION     DAOQUC010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3336
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32733_length_3336_cov_3.352027"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /gene="ligA"
                     /locus_tag="PKH07_05980"
     CDS             <1..258
                     /gene="ligA"
                     /locus_tag="PKH07_05980"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205556.1"
                     /note="this protein catalyzes the formation of
                     phosphodiester linkages between 5'-phosphoryl and
                     3'-hydroxyl groups in double-stranded DNA using NAD as a
                     coenzyme and as the energy source for the reaction;
                     essential for DNA replication and repair of damaged DNA;
                     similar to ligase LigB; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HNT34534.1"
                     /translation="EKPEARPHTSPIAGKTFVITGTLEGMSRDEAKAFLESYGAKVTG
                     SVSAKTDFLLCGTDAGSKLTKAQQLGVRVIELTELRSLLGE"
     gene            291..>1333
                     /locus_tag="PKH07_05985"
     CDS             291..>1333
                     /locus_tag="PKH07_05985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding family 9-like protein"
                     /protein_id="HNT34535.1"
                     /translation="MRKTRFFIPSLVSLLGLVFLFPVLGWTLEHGSVAVNTLAIPWLK
                     APKDFPGTLDKGCWTEAAQTTGFLDLQGRKMAANQTRLFMFYTDDSLWAAFRCLEPEN
                     SKMVLQVKEKDGPVWRDSSVEVFLDPMQTRKEYFQFVVNPLGILYDGKNMDGTWNSKA
                     QINSVTDEKGWTAFLRIPFADLGVTPKQGDVWVANFCRSRTIEDRTSWSRLIGSYTDP
                     VRFGQILFAGKNFKPLQVTEIKPLRMGKNDFPVQKGAFHFRIAGNNVKFERVFSIETE
                     GRDSCAFTLNNDEVQSLTIGLKDRDGRDLAEMYLPMESSRLSADASQLKRTVSVIKAQ
                     KMGFPEAMQQRII"
     assembly_gap    1334..1343
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1344..3065
                     /locus_tag="PKH07_05990"
     CDS             <1344..3065
                     /locus_tag="PKH07_05990"
                     /inference="COORDINATES: protein motif:HMM:NF024716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HNT34536.1"
                     /translation="VVNSAEPILEQVERMLKDPDGIDPDLWEKKATELQVLRMRLNSA
                     RALAETLVLYPDARFGLGIESSMKKVLIKDVPFDGWFANHYDLSLARYEREGFQIVII
                     PFDKSLKNVTVKATDLVKAGQLEPRATVQAKVSLVGHIRVEHRTPYEKEVHDWWPDPL
                     LDFQQSCDVNVEDQVAFWITVSTQSDTAAGDYLGQITVSADECPPLALNLKAHVWDFE
                     LPLLTSLRNAFTFDVHAMRKFYREKKDQEIVRKYHDFLLDYRLNIDHLYRKDPEDIEV
                     LKYGAKRGMNAFNICFTNGPERVKTIDEFVPQLKEAGLYDYAYVYGFDEVKKEKFPEI
                     KQTFGEIHKRHPGLETMTTADDKTFGKQSGLRDYVDIWVPLTWTYDLEEARQLRAEGK
                     EMWWYICVNPFHPYANWFIEYPAIESRLLTGSMSYMSETGGFLYYLINLWGNNKECIQ
                     SGPYTTWDGACLEYRPGTLWANGDGSLMCPGPDGPLSTIRLENIRDGFEDYDYFKTLV
                     ALRDKALRSEQSDAVKAFIKQADEALAVPVSLVKSLTEFTYDPTEVYSHRQKIAEAIV
                     AGRALGQ"
     gene            complement(3143..>3336)
                     /locus_tag="PKH07_05995"
     CDS             complement(3143..>3336)
                     /locus_tag="PKH07_05995"
                     /inference="COORDINATES: protein motif:HMM:NF023459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3520 domain-containing protein"
                     /protein_id="HNT34537.1"
                     /translation="LGMVLRDSQYKGASTLDDVLAIAQGSVGKDTWGYRSEFVSLVEQ
                     AKQLSTPAEVTAEVESATE"
BASE COUNT          813 a          869 c          885 g          759 t
ORIGIN      
        1 gaaaagcccg aagcacgacc gcacaccagc cctattgccg gcaagacctt tgtcattaca
       61 ggcactctcg aaggcatgag tcgcgatgag gcaaaggcgt tccttgaaag ttacggcgca
      121 aaagtcacag gctctgtcag tgccaaaacc gattttctgc tctgtggaac tgacgcaggc
      181 tccaaactca cgaaggcgca acaattgggc gtgcgtgtca ttgaacttac ggaactccgc
      241 tcccttttgg gcgagtaaat ctggaactct cgacaaggag gatttgcatc gtgagaaaaa
      301 caaggttctt cattccgtca ttggtatctc ttcttgggct tgttttcctg tttccggtgc
      361 tgggttggac gctggagcat ggttcggttg ccgtgaacac actggcaatc ccctggctca
      421 aggcaccgaa ggacttccct ggaacgctgg acaaaggctg ctggactgag gcggctcaga
      481 cgactggatt ccttgacctt caggggagga agatggccgc caatcagacc agactcttca
      541 tgttctacac cgacgattcc ctctgggctg cgttccgatg tctggagccc gagaacagca
      601 agatggttct ccaagtcaaa gaaaaggatg gtcctgtctg gcgtgacagc agtgtggaag
      661 tgtttcttga tcccatgcag acgcgaaaag agtacttcca gttcgtcgtc aatcctctgg
      721 gtattctcta cgatggaaag aacatggacg gcacctggaa ctccaaagcc cagatcaatt
      781 ccgtcaccga cgagaaaggc tggacggcat tccttcgaat cccctttgcc gatctcggtg
      841 tcactcccaa acagggggat gtctgggtag ccaacttctg tcgttcgcgc acgattgagg
      901 acaggacatc ctggtcacgg ctcatcggct cctacaccga tccggtgcga tttggacaga
      961 ttctcttcgc ggggaagaat ttcaagccgc tgcaagtcac agagataaaa ccgcttcgca
     1021 tgggcaagaa tgacttccct gtgcagaagg gcgcttttca ctttcggatt gcaggcaaca
     1081 atgtgaagtt cgagcgtgtc ttctctattg aaaccgaagg cagagactca tgcgccttca
     1141 cgctaaacaa cgatgaggtt cagagcctta ccataggcct gaaagataga gacggtcggg
     1201 atctcgccga gatgtatctc ccgatggagt cttcacgctt atctgctgat gcttcacaac
     1261 tgaaacgaac ggtgagcgtg atcaaagcgc agaaaatggg attcccagaa gccatgcaac
     1321 aaaggatcat ccannnnnnn nnngttgtga attctgctga acccatcctg gagcaggtcg
     1381 agaggatgct gaaagaccct gacggaattg accccgatct gtgggagaag aaggcaacgg
     1441 aactgcaggt actgcgaatg cgcctcaaca gcgcgcgagc attggcggag acactggtgc
     1501 tctatcccga tgcgcgcttt gggcttggga tcgaatcctc catgaagaag gttcttatca
     1561 aggatgtgcc gttcgatggt tggtttgcca accactatga tctctcgctt gcacgctatg
     1621 agcgcgaagg cttccagata gtcatcatcc cgtttgacaa gtcactgaaa aacgtgacgg
     1681 tcaaagcgac cgaccttgtc aaggccggac agttagagcc tcgcgcgacc gtacaggcca
     1741 aggtttcatt ggttggacat atccgtgtcg agcatcggac accctatgag aaggaagtcc
     1801 acgactggtg gccagatccc ttgttggatt tccagcagtc ctgcgacgtc aatgttgaag
     1861 atcaagtcgc tttctggatc acggtctcca cacagtcaga cacagcagca ggtgattacc
     1921 ttggacagat cacggtttcc gctgacgagt gtccaccgct ggcactgaac cttaaagccc
     1981 atgtctggga ttttgagctc cccttgctga catcgctccg aaatgcgttc actttcgatg
     2041 ttcacgccat gcgcaaattc tatcgcgaga agaaggatca ggaaattgtt cgcaagtacc
     2101 atgactttct cttggattac cgactgaata ttgaccatct gtatcgcaag gatccagagg
     2161 acatcgaggt gctcaaatac ggagccaaga gaggcatgaa cgctttcaac atctgcttca
     2221 ccaatggtcc cgagcgggtc aagacgattg atgagtttgt ccctcaactc aaagaggcgg
     2281 ggctttatga ctatgcctat gtctatggtt tcgatgaagt gaagaaagag aagtttcctg
     2341 agatcaaaca gacttttgga gaaatccata aacgccatcc agggctggaa accatgacta
     2401 ctgccgatga caagaccttt ggcaagcagt ccggcttgcg tgactacgtg gacatctggg
     2461 tgccactgac gtggacttac gaccttgaag aagccagaca actgcgagcc gaagggaaag
     2521 agatgtggtg gtacatctgt gtcaatccat ttcatcccta tgccaactgg tttatcgaat
     2581 atccagccat cgagtcaagg ctgctcacgg gctccatgag ttacatgtcc gaaacgggtg
     2641 gcttccttta ctacctgatc aacctgtggg gcaacaacaa ggaatgcatc cagtcaggcc
     2701 cctacaccac ctgggatggc gcctgtctgg agtatcggcc tggaaccctt tgggcgaatg
     2761 gagacggcag cctgatgtgt cccggtcccg atggcccgct ctcgaccatt cggctggaga
     2821 acattcggga tggttttgaa gactatgatt acttcaagac attggttgca cttcgtgaca
     2881 aagctctgag atcggaacag tccgatgcgg tgaaggcttt catcaagcag gccgatgagg
     2941 cactcgccgt tcctgtgtca ctcgtcaagt cgctgaccga gttcacatat gatcccacgg
     3001 aagtctacag ccatcgtcaa aagatcgccg aggcgattgt tgctggaaga gccttgggtc
     3061 agtagacaga gttcaaagag agaggccgcg ttcccgaccg tctgtcgaga atgcggcctg
     3121 ctcctcaatg cactcattct cactattcgg tggcagactc cacttcggca gtcacttcag
     3181 caggagtgga aagctgcttt gcctgctcga ccagactcac gaattctgaa cgatatcccc
     3241 aagtatcttt ccccacactt ccctgggcaa tcgcgagaac gtcatcgagc gtggatgcgc
     3301 ccttgtattg agagtctctg agaaccatgc cgagcg
//
LOCUS       DAOQUC010000277         3335 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_32748_length_3335_cov_3.427744, whole genome shotgun sequence.
ACCESSION   DAOQUC010000277 DAOQUC010000000
VERSION     DAOQUC010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3335
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32748_length_3335_cov_3.427744"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="PKH07_06000"
     CDS             <1..235
                     /locus_tag="PKH07_06000"
                     /inference="COORDINATES: protein motif:HMM:NF015291.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HNT34538.1"
                     /translation="LQALNSEYADKRTSGRLDPPVLKVVQRGESLRYRRQRVMNGTND
                     GQFKTLKLTDNPDYANHFTLEEEMSLSEVVSDT"
     gene            complement(222..1523)
                     /locus_tag="PKH07_06005"
     CDS             complement(222..1523)
                     /locus_tag="PKH07_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HNT34539.1"
                     /translation="MAKITFLGAGSTIFAKAVLGDSMLTPSLHDARIALLDIDPQRLG
                     DSEAMLNNIKATLGAKVKIEAFLAEDARKALKGADFVINAIQVGGYEPSTVIDFEIPK
                     KYGLRQTIADTLGIGGIFRGLRTLPVILEYCRIMEKVCPDVWFLNYVNPMAIVTGGVL
                     KGTSIKTVGLCHSVQACAHHLCHMLELPTDDLQWNIAGINHQGWLLEISRHGVDLYPE
                     IKRRAQLPEYVHKDTVRFEIMKRFGHYITESSEHTSEYLPYFIKQRCPELIESFHIPL
                     DEYPRRCINQINNWAKMRQDLVNSKTLEHHRTGEYASYIMNSVLTGELFKIGGNVLNK
                     HLITNLPYDCCVEVPCLVDRNGVQPCFVGNLPPQCAALTRTNVNVQELTIEAALTLKK
                     EHVYHAALLDPHTAAELTIDEIVAMCDELIEAHGDFLPKYH"
     gene            1722..2972
                     /gene="lysA"
                     /locus_tag="PKH07_06010"
     CDS             1722..2972
                     /gene="lysA"
                     /locus_tag="PKH07_06010"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HNT34540.1"
                     /translation="MNMPKSFEIQDGRILWSGQDLTQIAEAAGTPCYVYNQSVIEESY
                     RAYDEHLSSIPHLICYAMKANSSMAVLKVLADVGSSVDVVSGGELFRAQKAGLPVKKA
                     IFSSVGKGEDELREAVRAEILFFNIEAAEELDLLARIAEEEGKVAGVSVRINPDIDPG
                     THPYISTGLEMNKFGLVPDQALDVYRRAFRMPQLKVLGIQAHIGSQICSIQPFMDSLD
                     RLLFLRDKLESEGISLQYLSVGGGLGIQYNDENPPSPGDYADALAKRMQDTGLTLIME
                     PGRSIVGPGGILLTRVLYCKQTPRKRFVIVDGAMNDLVRPSLYKSYHRIVPVVLREGQ
                     SAKVDVVGPVCETGDFLAQDREMPSVQPGDLLAVCDAGAYGYTMSSNYNSRPKLPEVL
                     VNKKGFRIVRTRQTYEDLIAGESL"
     gene            3050..>3335
                     /locus_tag="PKH07_06015"
     CDS             3050..>3335
                     /locus_tag="PKH07_06015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34541.1"
                     /translation="MLLDKRKHWITLALALAVIGIWTILLRDVRDLFLEKETSEEAQR
                     DGSLSPENWPMFRGTPSLQGYVDAELPRKLFLEWVFKTKGAIRSSAAVVNN"
BASE COUNT          799 a          853 c          900 g          783 t
ORIGIN      
        1 tctccaggcg ctcaactcag aatatgcgga caaacgaact tcggggcgtc ttgatccgcc
       61 cgtgctcaag gtggtgcaga gaggtgaaag tctccgatac cgccgccaac gtgtcatgaa
      121 cggcaccaac gacggtcagt tcaagacgct gaaacttacc gacaatcccg actacgccaa
      181 ccacttcaca ctggaagagg agatgagcct ctccgaagtc gtcagtgata cttaggcaga
      241 aagtctccgt gggcttctat gagttcatcg cacatcgcaa cgatctcatc aattgtcagt
      301 tccgcagcgg tatggggatc cagaagcgcc gcgtggtaga cgtgctcctt cttcaaagtc
      361 aacgccgcct cgatagtcaa ctcctgcaca ttgacattgg ttcgcgtcaa tgcggcgcac
      421 tggggcggta gattcccaac aaagcaaggc tgaacaccat ttcgatccac aagacacgga
      481 acttcgacgc agcagtcata ggggaggttg gtgatcagat gcttgttgag cacattgcca
      541 ccgatcttaa acagttctcc tgtgaggaca ctgttcatga tgtaggaagc atactctccg
      601 gttcgatgat gctccagagt cttgctgttg accagatcct ggcgcatctt tgcccagttg
      661 ttgatttggt tgatacaacg ccgagggtat tcatcgaggg ggatgtggaa gctctcgatc
      721 agttccggac aacgttgctt gataaaatat ggaaggtact cggaagtgtg ctcgcttgat
      781 tcggtaatgt agtggccaaa acgcttcatg atctcaaagc ggactgtgtc cttgtgaaca
      841 tactcgggca attgggcacg cctcttgatt tccggataga gatccacgcc gtggcggctg
      901 atctccagca gccaaccttg atggttgatg ccggcaatgt tccactgcaa gtcgtccgtg
      961 ggaagttcca gcatgtggca cagatgatga gcacacgctt gaacgctatg acaaaggcca
     1021 accgtcttga ttgaagtacc cttcagaacg ccacccgtca cgatagccat cggattgaca
     1081 tagttgagaa accagacatc gggacaaacc ttttccatga tacggcaata ctcaaggatg
     1141 accggcaagg tgcgcaaacc ccgaaagatg ccgccgatgc ccaatgtgtc ggcgatggtt
     1201 tgcctgagac cgtacttctt gggaatctca aagtctatga ctgttgacgg ttcgtagcca
     1261 ccgacctgaa tggcgttgat cacaaaatcg gcacccttga gcgctttgcg cgcatcttcg
     1321 gcaagaaagg cttcgatctt gaccttggcg cccagagtgg ctttgatatt gttcagcatc
     1381 gcttcggaat ccccgagtcg ctgcgggtca atatccagaa gcgcgattcg tgcgtcgtgc
     1441 agcgatgggg tcaacatgga gtctccaaga actgctttgg cgaaaatagt actgccagca
     1501 ccgagaaaag taatctttgc catccaaaat cctccaataa gcggcgtcaa gtggacacca
     1561 gagtgcattg tcaaaaacgt ggggataata tgcttgaaca tcattgctgg caagccgatt
     1621 caaaccgtca cgacatctgc gcttgtttgc catgtcgttc tttgttatct ttgcccgagc
     1681 tggaagatct cttacgagaa acgggagtcg gaattgtgcc gatgaatatg ccaaagagtt
     1741 ttgagattca ggatggaaga attctgtggt ctggtcagga tctgactcag attgctgaag
     1801 ccgcgggaac gccctgctat gtgtacaacc aatcagtcat cgaggaaagt tatcgtgcct
     1861 atgacgagca tctctcttcg ataccgcatt tgatctgcta cgcgatgaag gccaactcca
     1921 gcatggccgt actgaaagtg cttgccgacg tgggttcttc cgtagatgtg gtttcgggag
     1981 gcgagctgtt tcgagcgcag aaagcgggtc tgcccgtcaa gaaagccatt ttctccagcg
     2041 tcggcaaagg tgaggacgag ctgcgcgagg cggttcgcgc agagatattg ttcttcaata
     2101 ttgaggctgc cgaagagctg gatctgcttg ctcggatcgc agaagaagag ggcaaggttg
     2161 ccggtgtttc tgttcgtatc aatccggaca ttgacccggg gacacacccg tacatttcga
     2221 ccggtttgga gatgaacaag ttcggcttgg tgcctgatca ggcacttgat gtctatcgtc
     2281 gtgcatttcg gatgcctcag ttgaaggtct tgggcattca ggcacatatt ggatcgcaga
     2341 tatgttccat ccagcctttt atggactctt tggatcggct tctctttctc agagacaagc
     2401 tcgagagcga agggatcagc ctgcagtacc tgagtgttgg cggaggatta gggattcagt
     2461 acaatgacga gaacccaccc agcccaggag actatgcaga cgccttggca aaacgaatgc
     2521 aggacaccgg tttgacactt atcatggaac caggccgctc catcgtcggc ccggggggaa
     2581 tcctgctgac acgggttctt tactgcaaac aaacccctcg caagagattc gtcattgtgg
     2641 acggagccat gaacgatctg gtgcgcccaa gcctctacaa gtcataccac cgaattgtgc
     2701 ctgtcgttct gcgtgaaggc cagtccgcca aagtggacgt ggtcggtcct gtttgcgaga
     2761 cgggagattt cctggcacag gacagggaga tgccttccgt ccaaccaggg gatctcctcg
     2821 cggtctgtga tgcaggtgcc tatgggtaca caatgtcttc gaattacaac tcgagaccca
     2881 aactccccga agtcctcgtc aacaagaagg gcttccggat agtccgaaca cgtcagacat
     2941 acgaagatct gatcgctgga gagtctctct aacacgatcg gcttgggcaa cgtctggtgt
     3001 ccacaaccgc ttgaatccct cgtgtcactg cacacaaagg aagattctta tgcttttgga
     3061 caaacgcaaa cattggatca ccttggcgct cgcccttgct gtgattggca tctggaccat
     3121 tttgctgcgt gatgtgcggg atctgttcct tgagaaggaa acttccgagg aagcccaacg
     3181 agatggatca ctgtcacctg aaaattggcc tatgttccgt ggcactccct ctctgcaggg
     3241 ttacgtggac gcagaacttc ccaggaaact cttcctggag tgggtgttca aaaccaaagg
     3301 agccatcagg tcatcggcag cggttgtcaa caaca
//
LOCUS       DAOQUC010000278         3331 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_32793_length_3331_cov_2.664835, whole genome shotgun sequence.
ACCESSION   DAOQUC010000278 DAOQUC010000000
VERSION     DAOQUC010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3331
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32793_length_3331_cov_2.664835"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1624
                     /locus_tag="PKH07_06020"
     CDS             <1..1624
                     /locus_tag="PKH07_06020"
                     /inference="COORDINATES: protein motif:HMM:NF013959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="integrin alpha"
                     /protein_id="HNT34542.1"
                     /translation="PTSVFSENLVSLDGVAVKPRLSECWRRTRHPRAGIRVALMVCLM
                     ATIQIAVLCSSAEAFNASERSNTVIYGATDSQRLGFAVAAGDVDNDGRDDWLLGTLSG
                     GIGKAYLFLSSQAHSDRISLATQAADVTFVGTKDYDDFGSALAIGDWNGDGVDDVIIG
                     SPGADYGGRSSCGVVYVFYGGTFTSGSVIDLSTQSPHVRVVGVAAGDGFGVSLSTGLF
                     NSDGYADLFAVADYGWSGKGAAYAITGGSDPAGTVIDVASSSRVQIKVYNAFSYDGIG
                     ECVGAGDINGDGRQDMILGISFAAPTFVNFLRGRIAVVFGGNTTRTINLASQNPDLTF
                     WGTSDKDAVGQAVTSGDLNGDGIDDLIFQANSDRAREEDPGIVYVLNGRTTWTPGSSY
                     TMSSVKPDVKILGEADYDGFGESLTVCQVNNAGPSDLIIGAFSHSTDPALPQEGAVYL
                     LPGNSNWQTGHVVYAGSSPEIALKITGGADTRCGWAVACGNFDGDKYRDLLIGAPIGD
                     FGASGFVGRCFVINYDTVFPRSSVDGFWMLLR"
     gene            complement(1651..3054)
                     /locus_tag="PKH07_06025"
     CDS             complement(1651..3054)
                     /locus_tag="PKH07_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNT34543.1"
                     /translation="MAEVLILDDDELFCQVLTEHIHRAGHGVSVSHTLAEGSKRASET
                     SYDIVFLDVHLPDGSGLDILSELRNSFLPPEVIIVTGYGDMEGAELAIKHGAWDYLSK
                     GASIRALELSFARALQHRESQRGAARRTLFHAPDMIGNSASFRAALSVAAQAADSDAS
                     VVVTGETGTGKELCAIAIHSNSRRAANPFVVVDCTALPETLVGSVLFGHARGAFTDAH
                     DDHIGLIGQAHRGTLFLDEVGDMPMEVQKSFLRVLQERCYRPVGADETHTSDFRVIAA
                     THRNLDDMVSQGTFRRDLLYRLRALTVSLPPLRERMDDLPSLITHFVMWQCKALDRDM
                     MGINDGFLQTLMQYSWPGNVRELSQAIVSAVVNAGDEPTLFASHLPTHIRTSVIQAGI
                     QGDGTPSRLSPENAAIFSWASELPPLREVRAQLDQTYLEEAIRRSGSDIEMLCQLSGL
                     SRPHVYVLLKKHGLKLK"
     gene            complement(3054..>3331)
                     /locus_tag="PKH07_06030"
     CDS             complement(3054..>3331)
                     /locus_tag="PKH07_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNT34544.1"
                     /translation="LTFMEKPDDFDLVLTDMTMPKMSGLELADEIHRVRPDARVILCT
                     GYGEENLKQLVTQGRIEKFILKPATIRKIALTVRQVLDRSVQSPEDC"
BASE COUNT          748 a          875 c          976 g          732 t
ORIGIN      
        1 tcccacctca gtcttctcag agaatctcgt atcccttgat ggtgtggctg tgaagccgcg
       61 gttgtccgag tgctggcgtc ggactcgtca tccccgagcc ggtattcgag ttgcgctgat
      121 ggtctgcttg atggcgacga ttcagatcgc agtcctctgt agctctgcgg aagctttcaa
      181 cgcttcggag aggagcaata ctgttatcta tggagcgacc gattctcagc ggttgggttt
      241 cgctgttgcg gcaggcgatg tggacaatga cggacgggac gactggcttc tgggaaccct
      301 gtcaggcgga atcggaaagg cctacctgtt cttgtcatcc caagcccact cggacagaat
      361 cagtcttgcc actcaggctg cggatgtgac atttgtcggg acaaaggact acgacgattt
      421 cggatcagcc ttagccattg gcgattggaa cggcgatgga gttgacgacg tgatcattgg
      481 atcgccggga gcggactacg gcgggcgaag cagttgcggc gtggtgtatg tcttctatgg
      541 aggaacgttc acgtcgggaa gcgtgatcga tctgtcaaca cagagtccgc atgtgagagt
      601 cgttggcgtt gcggcgggag atggctttgg cgtttcgctg tcaaccggac tgttcaacag
      661 tgacggatat gccgacttgt tcgcggttgc cgactatggc tggagcggga agggtgccgc
      721 atacgccatc actggaggtt ccgatccagc gggaacggta atagatgttg cttcatcttc
      781 aagggtccag ataaaggtgt acaacgcatt ctcctatgat ggcataggcg aatgcgtggg
      841 ggcgggagac ataaacggcg atggcagaca ggacatgatc ctcggcatct cgttcgcagc
      901 gccgacattc gtcaactttc tccgcggcag aatcgccgtt gttttcgggg gcaacaccac
      961 acgaacaatc aaccttgctt cgcagaatcc cgatctcact ttctggggga caagtgacaa
     1021 ggacgcagtg ggtcaggcgg tgacgagcgg cgatttgaat ggcgacggaa tagacgatct
     1081 catctttcag gcaaacagcg accgagccag agaagaagat ccgggcatag tctatgtgtt
     1141 gaacggcagg acaacgtgga cacctggctc atcgtacacg atgtcctccg tcaagccaga
     1201 cgtcaagatt ctgggtgagg cagactacga tggctttggg gaatctttga ctgtatgcca
     1261 agtcaataat gccggcccct cggatctcat catcggcgcc ttttcacatt ccacagatcc
     1321 ggctctccct caggaggggg cggtttacct gctccctgga aactcaaact ggcaaacagg
     1381 acacgtcgtg tacgcggggt cgtcaccaga gatcgccctg aaaatcacag gcggcgcgga
     1441 cactcgatgc ggctgggcgg ttgcgtgcgg gaattttgac ggggacaagt accgcgatct
     1501 tctgattggt gcccccattg gcgacttcgg ggcgtccgga tttgtcggac gttgcttcgt
     1561 cataaattat gatacggtgt tccctcgttc cagcgtagat ggattctgga tgttgttgcg
     1621 ctgagacgct acactcgaag cccgaactga ctacttgagt ttcagcccgt gttttttgag
     1681 cagcacatag acgtgggggc gggaaaggcc tgagagttgg cagagcattt cgatatcgct
     1741 gcccgatcgt ctgatcgctt cttcgaggta ggtctgatcg agttgagcac gtacctcacg
     1801 caaaggggga agttcgcttg cccaggagaa aatcgctgcg ttttctggtg acagtctcga
     1861 aggggtgccg tcgccttgga taccggcctg aatgacactc gttcggatat gcgttggaag
     1921 atgactggca aagagagtcg gctcgtcgcc agcattcacc acagcactaa cgatggcttg
     1981 agacagttcc cgaacattgc ccggccagga gtactgcatc aaggtctgaa ggaatccgtc
     2041 attgattccc atcatgtccc tgtcgagtgc cttgcactgc cacatcacaa agtgtgtgat
     2101 gagcgaagga agatcatcca tccgttcccg caatggtgga agtgaaaccg tcagggcgcg
     2161 aagccgatag agcagatccc gacgaaaagt cccctgggag accatgtcat cgaggttgcg
     2221 gtgagtcgcg gcaatgactc tgaagtcgct ggtatgtgtt tcatcagctc cgacggggcg
     2281 atagcagcgt tcctgaagaa cacgaaggaa ggatttttga acctccatgg gcatatctcc
     2341 gacctcatcg aggaacagag ttccgcggtg cgcctggccg atcagaccaa tatgatcatc
     2401 atgcgcgtcg gtgaaggctc cacgagcgtg tccgaaaagg acactgccca ccagcgtttc
     2461 gggcagtgcg gtgcagtcaa cgaccacaaa gggattcgcg gcgcggcgac tgttggaatg
     2521 gatcgcaatg gcacacaatt ccttgccagt gccggtctct cccgtcacca caacacttgc
     2581 gtcactgtcc gccgcttgag ccgcgacgga cagcgctgcc cgaaaagagg cggaattccc
     2641 gatcatgtcc ggagcatgaa acaaagtccg tctggcagca ccacgctggc tctcgcgatg
     2701 ctgaagcgcc ctcgcaaaag acagttccaa ggcacgaatc gaagcgcctt ttgagagata
     2761 atcccaagct ccatgtttga tcgccagttc agcaccttcc atatcgccat atcccgtgac
     2821 aatgatgact tcaggcggca agaaggagtt tcgcagttcc gagaggatgt ccagtccgga
     2881 gccatcgggg agatgcacat cgaggaagac gatgtcgtag gacgtttcgg acgctcgttt
     2941 ggatccttca gcaagcgtgt gtgaaacgga aacaccatgc ccggcacggt ggatgtgttc
     3001 cgtcagcacc tgacagaaca gttcatcgtc gtcaagaatg agcacttcgg ccatcaacag
     3061 tcctccggac tttgcacaga gcggtcaaga acctgacgaa cagtcaaggc gatcttccgg
     3121 atggtcgccg gcttcaagat gaacttctcg atacgacctt gagtgacaag ttgctttaag
     3181 ttttcttcac cataccccgt acagagtatc acacgagcat caggacgcac acggtggatt
     3241 tcatcggcca actccagtcc gctcatcttg ggcatggtca tatccgtcag cacgagatca
     3301 aaatcgtctg gcttctccat aaaggtgagc a
//
LOCUS       DAOQUC010000279         3328 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_32842_length_3328_cov_2.751910, whole genome shotgun sequence.
ACCESSION   DAOQUC010000279 DAOQUC010000000
VERSION     DAOQUC010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3328)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3328)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3328
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32842_length_3328_cov_2.751910"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            24..224
                     /locus_tag="PKH07_06035"
                     /pseudo
     CDS             24..224
                     /locus_tag="PKH07_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010417056.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
     gene            360..617
                     /locus_tag="PKH07_06040"
     CDS             360..617
                     /locus_tag="PKH07_06040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34545.1"
                     /translation="MNPRRIILSEIFHLQISTLTFFRLNSRIQVLMLKSSFTTILQVD
                     VEWISQQLLIDDFLLAKFQRFTRWAHLNDFFLVVLIAIFLA"
     gene            complement(801..872)
                     /locus_tag="PKH07_06045"
     tRNA            complement(801..872)
                     /locus_tag="PKH07_06045"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(837..839),aa:His,seq:gtg)
     gene            complement(884..963)
                     /locus_tag="PKH07_06050"
     tRNA            complement(884..963)
                     /locus_tag="PKH07_06050"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(926..928),aa:Glu,seq:ttc)
     gene            complement(1054..1137)
                     /locus_tag="PKH07_06055"
     tRNA            complement(1054..1137)
                     /locus_tag="PKH07_06055"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1103..1105),aa:Lys,seq:ctt)
     gene            complement(1145..1219)
                     /locus_tag="PKH07_06060"
     tRNA            complement(1145..1219)
                     /locus_tag="PKH07_06060"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1184..1186),aa:Arg,seq:acg)
     gene            complement(1226..1301)
                     /locus_tag="PKH07_06065"
     tRNA            complement(1226..1301)
                     /locus_tag="PKH07_06065"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1266..1268),aa:Asp,seq:gtc)
     gene            complement(1382..1455)
                     /locus_tag="PKH07_06070"
     tRNA            complement(1382..1455)
                     /locus_tag="PKH07_06070"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1421..1423),aa:Thr,seq:ggt)
     gene            complement(1553..1858)
                     /locus_tag="PKH07_06075"
     CDS             complement(1553..1858)
                     /locus_tag="PKH07_06075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34546.1"
                     /translation="MLYSKLGLGWVPQISFAKEEGERKLVVATRYRGMNTGEYEVCRL
                     LYVDDKLVFNSCSGNDESKWVQADIFGIYSEFAEALSSINDPLTSKMKARLKRGGLK"
     gene            complement(2033..2107)
                     /locus_tag="PKH07_06080"
     tRNA            complement(2033..2107)
                     /locus_tag="PKH07_06080"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2072..2074),aa:Glu,seq:ttc)
     gene            complement(2115..2208)
                     /locus_tag="PKH07_06085"
     tRNA            complement(2115..2208)
                     /locus_tag="PKH07_06085"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2173..2175),aa:Asn,seq:gtt)
     gene            complement(2217..2290)
                     /locus_tag="PKH07_06090"
     tRNA            complement(2217..2290)
                     /locus_tag="PKH07_06090"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2256..2258),aa:Trp,seq:cca)
BASE COUNT          742 a          892 c          757 g          937 t
ORIGIN      
        1 cctaacgatg gagggcactg accatgtttg aaacattatc acacttaaca ctgcaacaat
       61 attggtggtt cctgatatcg ctattgggag gacttctggt atttttaatg ttcgtgcaag
      121 gcgggcaaac gctttttttc accctgagca aaacacgcga cgaacgcacc atgctcatca
      181 acgcgctggg gcgcaagtgg gaatttacct tcaccacgct ggtgaagact attcttggcg
      241 ccaatctttc ttttagtctc ttccttgtgt gtctttccag tccaatcgta aaatctcact
      301 tctcgtctgc cttcttcata tgcgcgcttc agactgtttg acagttttct ggatttttgt
      361 tgaacccacg ccgtatcatt ctctctgaga tatttcatct tcagatttcc acgcttacct
      421 ttttcagact gaacagcaga attcaagttc tgatgctcaa atcctccttc accaccatac
      481 ttcaagttga tgttgagtgg atcagccagc agttgctcat tgacgatttc cttctcgcga
      541 agtttcagcg cttcacgcga tgggcacatc tcaatgattt ctttcttgtg gttctcatcg
      601 ccatatttct tgcgtgagta acccagaatt ttcccactac caagataccc atcatcgaga
      661 ttatcggtgc tgtgcatccc tacatagaac ttgcccgtga cttggcaggt ggttttgtag
      721 atataatgat acttcttctg tcggcgcata ttgttctcct ccacctattt acaggcttgg
      781 ggtacaatac gacctgaatg ggtgtctgag gaggatcgaa ctcccacttc cagggtcaca
      841 acctagcgtg cagaccacta cactacagac accattgttc ttgagcaccg tggggaatcg
      901 aaccccagtc cgaagacgtc gccttgaaag ggcgaggacc gttaccagca gtcatcacgg
      961 tgcattgttc ttggtcccgc gcccaggact cgcacctggt tccagccgct taagaggcgg
     1021 agcttcactg tcaaagtttg cacgggttgt atttggtggg ccgcctgggg tcgaacccag
     1081 atagggattg ctcccacgga ttaagagtcc gttgcataac cgctctgcca acggcccaat
     1141 gttatggcac ccccggatgg aatcgaacca ccgtctgaac gttcgtagcg tcctattctc
     1201 tccgttgaac tacgggggca ttgtttggcg acgtatacgg ggatcgaacc cgtctagccc
     1261 gcatagacag tgcggtggtc tcccagagag ccgaatacgc catgttcttg ccatcgagcg
     1321 ccaggtgtcc cacacgccct cattttgtcg ctccttgcgg agactcgatg gactttcatg
     1381 ttggtgccca agtcgagaat cgaactcgct tttcgcccat accaggggcg tgttctacca
     1441 ataaactaca agggcattat gtttggcgca gaccgtccaa gacgagaggt ggcgcggggt
     1501 cacttcgcgc ggtagccaca acccttacga gacctttcac ctgtctgcat tgtcatttca
     1561 atccaccacg cttcagccgc gctttcatct ttgatgtcag cgggtcgttg atggaactta
     1621 gagcttcagc gaactcagag tagatgccga aaatatcggc ttgaacccat ttgctctcgt
     1681 cattgcctga gcaggagttg aaaaccagtt tgtcatctac atacagcaag cgacaaactt
     1741 catactctcc agtgttcata cctcggtacc gggtggcaac gacaagcttt cgttctcctt
     1801 cttctttggc gaaggaaatc tgtggtaccc accctagtcc caactttgaa tagagcatga
     1861 gagcatcaag ttttgctctc atgctctaaa gagaacgctt tgtcagctgc gatcttcttg
     1921 agtctgtcga agaccttctg aatcttagca atcgtttctt cttcgctgct ttcaagcagt
     1981 tcatctaaca gcatttcaaa tccctcagga agattactgc agatatttat gatggtacac
     2041 cgtatgggac tcgaacccat ctctccggtt tgaaggaccg ggaatctcac ccgaaattca
     2101 acggtgcatt gtcttggctc cccgggaggg cttcgaaccc catattacgc cttgcggcct
     2161 ttacgtctgg ttaacagcca ggtctcttac cagttagagt accggggaat tgaatttggc
     2221 agaccgccag ggaatcgaac cccgtccaaa ggctttggag gcccctatgc taccggaaca
     2281 cctgcggtcc attgttcatt ttcaaagatc cgtcccagcc gatgctatcc gtgtggaagg
     2341 agccgcgtac gccgtcacca acttctatca aggtggacct ttgaaaatga acccgtcgta
     2401 agcgcagtct ccacacctca ggtgcagtag ctactccgcg acggcttcaa aatgccatcc
     2461 ctatctcgca ctcgctctgc ctcatgtagg ttcaccggca gggtcatagc ggtccagctt
     2521 gtcttctata ccgcgaacac tttcaacgcg gtttcttctg ctccaaaagc aactcaggga
     2581 accgttttac tggttccctg atcttcttgg tgcttgaggt tgctgtttag gctttcctct
     2641 ccccgaagat cagggctgtc gtatcgatct cgtggcgctc aaagcgcacg acggtattct
     2701 ggcgctgttg ccagaacatc tccgtggttt tgatgcgatt gaagaccgag ttcatgatgt
     2761 tgtccgtttt ccttgcggaa gtttgtttgc gttgatgaag ttatttatat gagaagtcag
     2821 ttttcttgcg aaacaaaaac caggttgccg ttattacgag caaacgtctc gtcaagattg
     2881 acatgagcgg cattcgcttt ctgtctttcc atctgtcgta ttgcttcttc caagacggca
     2941 gacacactca acgaccaaac acgtttaaga cttagcgaac tattcttatc gaagacagcg
     3001 agaaagatca agtcgtttct cgttattctg tgcaacgatt tcatcgcttt tcctccggag
     3061 acttgagcat cctatcaagt tggcctgctc caccttccca gcaggaaaga tattcgcaga
     3121 cttttccatc agatgttctt gcatccggcc cgtgcttctt tggtagaagt gcatgaccta
     3181 atgtgtacgc tactactgct tcctttgcta agcctggttg aaagatgtta ttgatgccat
     3241 gagacctcgc gaccatgtcg agatgtttca gcgcttcaat caactctatt tgcatttatc
     3301 cattatacat agggtttcgc aaaagtac
//
LOCUS       DAOQUC010000280         3320 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_32951_length_3320_cov_3.479326, whole genome shotgun sequence.
ACCESSION   DAOQUC010000280 DAOQUC010000000
VERSION     DAOQUC010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3320
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32951_length_3320_cov_3.479326"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(231..1652)
                     /locus_tag="PKH07_06095"
     CDS             complement(231..1652)
                     /locus_tag="PKH07_06095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34547.1"
                     /translation="MALPRRRVPSPVLAGVFFVACFLVIFVSGIARISASLCGMSKTF
                     LVLLFSSVGASLAGPYTYRCFERWADRRCEAFLARVAILLSFSIPFSLILIFLLWKLL
                     LLDHLMDFVVLSAVLAVPFLFSATIFIAVLSRFPREAGRLGLQISLGLVAGCMLGGFL
                     FHFLSDVYVTVLNGVFATLAAFAFSWERNLRKQRSAAVAMTLFVTALSYFKTDISFAR
                     LDNGVTHSPSGYVLLRSLYDLLPSESIFARMISSSVVVALLIACLFLVLFRQGRRLRS
                     SLACEKRMLLLYFVCVGIGLGIVGLSLFWEYSLFVADPLLASTLVLFILSASMATGTW
                     LVKKLALAGRRNAIHGTLLLTAGALACIITLYAGPFEDLVGLWKNAPALLRIPTVFLL
                     LFPFGMLVGVTVPLAWSFVREEMVPDLPKIAGSLSGMFSLGLLLALCLSLGQGFDVSL
                     MIGQVFLLMAFILVALMPQSPPT"
     gene            1972..2979
                     /locus_tag="PKH07_06100"
     CDS             1972..2979
                     /locus_tag="PKH07_06100"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNT34548.1"
                     /translation="MERSRRHKIVLYGQPDSYQEGLNHLSDQGHNVVVLQDINEVKRH
                     IYAEMSDVLLLDLPHLGDVGLTLCRRLKSDPRTYNKPIIVAFGRQHDSLELAAIQADV
                     DDFTMLPMAPTVLAARIQMIINRSSRQQMSNPLTGLPGNLAIEEHLNRKLSLGQPLAV
                     AYADLDNFKAFNDRYGYSRGDNVIRLTAMIVKEAIQNYGRQDDFYGHIGGDDFIMITD
                     WACLDQVCTHAITSFDTLVPFQYDEEDRVRGGVFATNRQGESVTIPVMTLSIGVVTNQ
                     KRELRNCLQIADLATEMKSHAKQFSRSLYLVDRRQEGVRMPETQSTEAQEPIADLEQE
                     S"
     gene            2976..>3320
                     /locus_tag="PKH07_06105"
     CDS             2976..>3320
                     /locus_tag="PKH07_06105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34549.1"
                     /translation="MSSKTEKPVQKSPQKPKIAITIGDPAGVGPEVVLKSLNLVERDF
                     DPILIGSLRILKDASERFRIAAKFRSWGGDAVRDSTPVVDVCPLSVPPEFGSVRREYG
                     EFVWKSIKVAVDM"
BASE COUNT          936 a          854 c          879 g          651 t
ORIGIN      
        1 gcagcccggc cactgccatg gaaccgaagc caatcacaac agcccagacc gcgtgttcgt
       61 cgtctgccgg cagtgagagc cgaagccatc tcagaattct aagtccatag cccaacgagg
      121 ctgtcaggaa gatgagaaaa tagaggagtt cgatcagagg agtgcctcca tttcagacgt
      181 tctatggtgc gaagaagatc ccagctgatg aactcatcca tttccacctt ctatgtgggt
      241 ggactctggg gcatcaaggc gaccaggatg aaagccatca ggaggaaaac ctggccgatc
      301 atcagggaaa catcaaatcc ctgtccgagc gacaggcaga gggcaagcag cagtcccaag
      361 gagaacatcc ctgagagact tcccgcaatt ttcgggagat caggaaccat ttcctcccga
      421 acgaaagacc atgccagcgg aacggtcact ccaaccaaca ttccaaacgg gaacagaagc
      481 aggaagaccg tgggaattcg aagaagagca ggagcatttt tccagagacc gaccaggtcc
      541 tcaaaaggtc ccgcgtacag agtaatgata caagccagag cacctgccgt aaggagaagt
      601 gttccgtgga tggcattgcg acggcctgcc aacgcaagtt ttttgaccag ccatgttcct
      661 gttgccatcg aggcactcag aatgaacaag accagagtcg aagcgagcag aggatctgcc
      721 acaaacagcg agtactccca gaagagtgac aaccctacga ttcccaagcc gattccaacg
      781 cagacaaaat acaacaggag cattctcttc tcgcaagcca gactcgatct caagcgccgt
      841 ccttgccgaa agagtaccag gaagagacac gcgataagaa gagcgacaac aacacttgac
      901 gaaatcatgc gtgcgaagat gctctcgctg ggaagcagat catacaaaga acgcagcagg
      961 acatatccgg acggcgaatg ggttacacca ttgtcgagac gggcaaaaga gatatcagtc
     1021 ttgaaataag acagagcggt gacgaacagt gtcatagcca ccgctgctga tcgctgtttc
     1081 ctcaaattac gttcccagga gaaagcaaac gcagctaatg tcgcaaagac cccattgaga
     1141 accgtgacgt agacatcgct gagaaaatgg aaaagaaatc caccgagcat acatcctgcc
     1201 accagaccaa gcgagatttg cagccccagc cgtccagcct ctctggggaa tcggctcaag
     1261 acagcgatga aaatagtggc gctaaacagg aacggcaccg cgagcactgc cgagagcacc
     1321 acaaaatcca tgaggtgatc cagcaggagc agtttccaga gcaagaaaat cagtatgagc
     1381 gaaaaaggaa tggaaaaact caacaagata gcgactcttg cgagaaaagc ttcgcagcgt
     1441 ctgtccgccc agcgctcaaa gcatcggtat gtataggggc ctgccagcga ggcaccgact
     1501 gacgaaaaca agagcacaag gaaggtcttg ctcataccac acagcgaagc agaaatgcgt
     1561 gcaatcccgg aaacaaagat gaccaggaag cacgcaacaa aaaacactcc ggcaagtacc
     1621 ggagatggca cacgccttcg cggcagagcc attcctcaga gcactcctca atgcttattt
     1681 gttcggcaaa gccatcgtga aggaaacagg tcctccccgg acccaatcct ctaatccaaa
     1741 agagagtata cctcaggttg tttgtagaga tcaatgctga aaaaacaact cagaaaccgt
     1801 tgacactttg ggttgtcggg atagaatgaa agtagtggag tccgatccgg tcgggcggtt
     1861 ctttttttgg caaccggtgc gctctggagt gcgagggcga tcagagtccc tctgagtttc
     1921 cgcaaggtta agaaaccaac ccatcctgat ggttggtgcg ccaggagaga tatggaacgt
     1981 tcgcgcagac acaagattgt tctttacggc cagccagatt cctaccagga aggactcaat
     2041 catctttccg atcagggcca caacgtggtt gttcttcagg atatcaatga ggtcaagagg
     2101 cacatctatg cggaaatgtc ggacgtattg ctgttggatc tccctcatct gggggatgtt
     2161 gggttgacgc tatgccgacg gttgaaaagc gatcctcgca cctacaacaa gcccatcatc
     2221 gtcgcttttg gaagacagca tgactcattg gaactggctg caattcaggc agatgtggac
     2281 gattttacga tgctgccgat ggcgccaacg gtcttagcgg cacgcattca gatgatcatc
     2341 aaccgcagca gtcggcagca aatgtccaac cccttgaccg gactgcctgg caaccttgct
     2401 atcgaagaac acctcaacag aaaactgagt ctgggtcagc ccttggcagt tgcctacgcg
     2461 gatttggaca acttcaaggc attcaacgat cgatacggtt attcccgtgg ggacaatgtc
     2521 atccgtctca ctgcgatgat tgtcaaggaa gcaattcaaa actatggcag gcaggatgac
     2581 ttctatggtc acatcggtgg agatgatttc atcatgatca ccgactgggc gtgcctggat
     2641 caggtgtgca ctcatgcaat cacctcgttt gacacccttg tgccgttcca atacgatgaa
     2701 gaagatcggg tgcgcggagg tgtttttgcc acgaaccgac aaggtgagtc ggtaacgatt
     2761 cccgtcatga ctctgtccat cggagtcgtt accaaccaga agagagaact ccgaaactgt
     2821 cttcagattg ccgatctggc cacggagatg aaatcgcatg ccaagcagtt ttcgagaagt
     2881 ctgtatctcg tagaccgccg ccaggaaggt gtgaggatgc ccgaaacgca gtcaactgag
     2941 gctcaggagc ccattgccga tttggagcaa gagagttgag ttcgaaaacc gaaaagcctg
     3001 tacagaagtc accccaaaag cccaagatcg ccataaccat aggggatcca gccggtgtgg
     3061 ggcctgaagt cgttctgaaa tccctcaatc ttgtcgagag agattttgac cccatcctga
     3121 tagggagtct tcgcatactc aaggatgctt ccgaacgctt tcggattgcc gcgaagtttc
     3181 gttcctgggg tggtgacgcc gttcgggact cgacgcctgt ggtggacgtg tgtcctctgt
     3241 ctgttccacc cgaatttggg tctgtaagaa gggagtacgg tgagtttgtc tggaagtcca
     3301 tcaaagtggc agtggatatg
//
LOCUS       DAOQUC010000281         3315 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_33029_length_3315_cov_3.153067, whole genome shotgun sequence.
ACCESSION   DAOQUC010000281 DAOQUC010000000
VERSION     DAOQUC010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3315
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33029_length_3315_cov_3.153067"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="PKH07_06110"
     CDS             <1..396
                     /locus_tag="PKH07_06110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34550.1"
                     /translation="VNESDRWEVGSFVEFLGDEQIKSALMEIVLSEPPASKPEKQWQD
                     CCDTLQRLQVEQEIQRQIQEFQNNPSVDYLREGQQQYQHSIQQHEMLWRSISETEPGD
                     RGSSAQSQVRKQRAASSAVEKHQDSEEQT"
     gene            397..2238
                     /gene="rpoD"
                     /locus_tag="PKH07_06115"
     CDS             397..2238
                     /gene="rpoD"
                     /locus_tag="PKH07_06115"
                     /inference="COORDINATES: protein motif:HMM:NF004208.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HNT34551.1"
                     /translation="MAVKKAKQSKASDKQTKKREEIKRLVEISQEHGHVTYDDINEVL
                     DDEDVNSEDLDEIFSTLADLNVEVVDDEKFDEDHAKVEPEEDRAEDSDDSQDALEIPL
                     KRAEAEVQSQARPPDRTKVDDPVRMYLREMGRVPLLTREQEVELAKRIEHGRHRVLRA
                     IAGARLTYLDLKRIYERLEASKLRIEDALQNMTLHQDEIEEERENILKDIFDKVEQIR
                     KLQREIHLTERRLAQPRLGKRQRSELSHRITGRRHKLGNVIISLNFDYEQVARTSEKL
                     LSYNEKIKECLSTIFQAESALSMDLAEMVKQHQRIGRMSPATLKKRGITKEQKDTLDT
                     WVSRAKKAREIIKRLETDSTLTSDEIDKVCKQIREGKHLAHIAKMEVVEANLRLVVSI
                     AKNYTNRGLQFLDLIQEGNIGLMRAVDKFEYRRGYKFSTYATWWIRQAVTRAIADQAR
                     TIRIPVHMIETINKMTRISRRLVQEFGREPTPEEISARMNMSVEKVRGVFKIAQQPIS
                     LETPIGDEGDTHFGDFIEDKNAVSPASAAEDLIMQEQVESVLKTLSPREETVLRLRFG
                     IGDGYQRTLDEVGNRFGVTRERVRQIETKALKKLRHPTRCKKLRDFI"
     gene            2590..>3315
                     /locus_tag="PKH07_06120"
     CDS             2590..>3315
                     /locus_tag="PKH07_06120"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HNT34552.1"
                     /translation="MSIYFLKLTVLAMLGVLLSFWPTREACANDGNEEPERCPTLSSS
                     LPKAASPCADLHLGFVAHWAWRPDVQEYLASGSTSMLMNLLCWGVTDKERQIVDFRPA
                     EAWLGDLYRSGLKGVLIIDTSLHHSSIGWRSEITKSLQENLCDNFGNQTIYSSHLSPK
                     FRQMVQQYIEDLTCWTMKNDVEHRIVAYTNGAEVFFPGILDHSRFAVEAFRIWLLERY
                     GSLDTVNERWGAGFRSIDEVEAPR"
BASE COUNT          867 a          795 c          965 g          688 t
ORIGIN      
        1 gtcaatgaat cagaccgttg ggaggtggga agtttcgtcg agtttttggg tgacgagcag
       61 atcaagtcgg ctcttatgga aattgtgttg tcggaacctc cggcgtcgaa gcctgagaaa
      121 caatggcagg actgttgtga tacgctccaa cgcctgcaag tggaacaaga aatccaaagg
      181 cagatacagg agttccagaa caacccaagc gtggactacc ttcgggaagg tcagcagcag
      241 taccagcaca gcattcaaca acatgaaatg ctgtggcggt caatttcgga gacggagcca
      301 ggcgatcgag gctcatcagc acagagtcag gtacgaaaac agcgtgcagc gagttcggcc
      361 gtcgagaagc atcaggattc agaggagcaa acctaaatgg ctgtgaagaa agccaagcaa
      421 agtaaggcgt cagacaaaca aacaaagaaa cgcgaagaga ttaagcggtt ggttgaaatc
      481 agccaggaac acggacatgt cacttacgat gacatcaacg aggtcttgga tgatgaagac
      541 gtcaactcgg aagatctgga cgagatattc agcacactgg cggatttgaa tgtcgaagtt
      601 gtggacgatg aaaaattcga cgaagaccat gccaaggtgg agcctgaaga ggatcgtgcg
      661 gaagactcgg atgactcgca ggacgccctg gagattcccc tcaaacgagc tgaggcggag
      721 gtgcagtccc aggccagacc gccagaccgc accaaggtgg atgaccctgt tcggatgtat
      781 ctgagggaga tgggaagggt tccgctgctg actcgagagc aggaagtcga gttggcgaag
      841 agaatcgaac atggacgcca cagagtgctg cgagccattg ctggagcccg actgacttat
      901 ctggacttga agcgcatcta tgaaagactg gaagcgtcaa aactcaggat tgaggatgcc
      961 cttcagaaca tgacccttca tcaggatgag attgaggaag aacgtgagaa catcctgaag
     1021 gacatctttg acaaggtgga acagatccgg aaacttcagc gcgaaatcca cctgacagaa
     1081 cgcaggttgg cacaacccag gctgggaaaa cgacaacgtt cagaactgag ccatcggatt
     1141 acaggccgtc gccacaagct tggcaatgtt atcatctcac ttaatttcga ctacgaacag
     1201 gtggcacgga cttccgagaa actgctcagt tacaacgaga agatcaagga gtgtctctcg
     1261 acaatcttcc aggcggaaag cgcgctcagc atggatttgg ccgagatggt caagcagcat
     1321 cagcggatcg gtcgaatgtc tcccgccact ctgaagaagc gtggaatcac caaggaacag
     1381 aaggatacgc tggacacttg ggtttcacgg gctaagaaag cccgggaaat catcaaacgt
     1441 cttgagaccg attcgaccct gacgagtgat gagattgaca aggtctgcaa gcagattcgc
     1501 gaaggcaaac acctggcaca tatcgccaag atggaggtgg tggaagcgaa tcttcggctc
     1561 gttgtctcga tagcgaagaa ctacaccaac cgaggacttc agtttctgga tttgatccag
     1621 gagggcaaca tcggtctcat gcgagcggtg gacaagttcg agtatcggag aggctacaag
     1681 ttttcaacgt acgccacttg gtggattcgt caggcggtca cgcgcgccat tgcagatcag
     1741 gcgcggacga tccgaattcc tgttcacatg attgagacga tcaacaagat gacgaggatt
     1801 tcgcggcgac ttgttcagga attcggtcgg gaaccgactc ctgaagagat ctccgcgcga
     1861 atgaacatgt cggtcgaaaa ggttcgaggc gtcttcaaga tcgcccagca accgatctcc
     1921 ctagaaacgc ccatcggaga tgagggagac acgcatttcg gggatttcat cgaagacaag
     1981 aacgctgtgt cacctgcctc agcagccgag gatctgatca tgcaggaaca ggtggaatcg
     2041 gtcctgaaga cactttcgcc acgtgaggaa acggtgcttc ggcttcgctt tgggatcgga
     2101 gacggctatc aacgaacctt ggacgaagtt ggcaaccgct ttggcgtcac tcgtgagcgt
     2161 gtccgacaga tcgaaaccaa ggcgctgaaa aaacttcgac atccgactcg atgcaagaaa
     2221 ctgcgcgact tcatctagga aggcgcgtct atcgcttctt cggatataga tttgtttcag
     2281 gcgggaatgg ttgagagcgg cttgagcgtt caaaactttc ccgcctttct ttcggggcgc
     2341 agacattgac ttgcaggcaa atactatccg accaaaatcc ttcctgcatt gacaccctcc
     2401 gaggagggaa ttctcggcca caggctgtcc agcgttgcgg tgactcgagt tgactggtac
     2461 tcttcagaga aaccggactc cggatgaggc ggtttctctt gcgagcaagc accgtacagg
     2521 agtgggcata gccacacgcc cgcaggcgaa tgcctgctcc gaagagacta ctacgcactg
     2581 aggtgggatg tgagcatcta ttttttgaag ttgactgttc ttgccatgct gggcgttctg
     2641 ctttctttct ggccgaccag agaggcctgt gccaatgacg gcaatgagga acctgagaga
     2701 tgcccaacgc tctcatcgtc acttcccaag gcggcgtcgc cctgcgctga tctgcatttg
     2761 ggatttgttg cccactgggc gtggcgtccc gacgttcagg aatatctggc gtcaggttcg
     2821 acctccatgt taatgaacct gctgtgctgg ggagttacgg acaaggaaag acagatcgtg
     2881 gactttcgac cggctgaggc ttggctggga gatttgtacc gaagcggctt gaagggtgtt
     2941 ctgatcattg atacgtcgct gcatcattcc agcatcggat ggcgctcgga aatcaccaag
     3001 agcctgcagg aaaatctctg cgacaacttt ggcaatcaga cgatctattc gagtcatctc
     3061 tcgccgaagt ttcggcagat ggttcagcag tacattgagg atttgacgtg ttggacgatg
     3121 aagaacgatg tcgagcacag aattgtggcg tacaccaacg gtgcagaagt cttcttcccc
     3181 gggatactcg atcacagtcg cttcgccgtt gaggcttttc ggatatggct tctggaaaga
     3241 tacggttcgt tggatacggt caatgagcga tggggtgcgg ggtttcgatc cattgacgag
     3301 gtcgaagcgc cacgg
//
LOCUS       DAOQUC010000282         3299 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_33265_length_3299_cov_2.415845, whole genome shotgun sequence.
ACCESSION   DAOQUC010000282 DAOQUC010000000
VERSION     DAOQUC010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3299
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33265_length_3299_cov_2.415845"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..855)
                     /locus_tag="PKH07_06125"
     CDS             complement(<1..855)
                     /locus_tag="PKH07_06125"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNT34553.1"
                     /translation="MRRQTSDKRHLKSSTWSLILLLAVFGIALSSCRRIDFGLMRQEH
                     TEAFTRELAERSTLELSSEQPIGLQQCIEIALRNNLNVKASEIDKRLASLNRKIAFSN
                     FLPHLDASFDTVSSLKHRMVKAGGGYVPMSDQTVTEGTLRAQQAIFAPQTWFLYDAYV
                     KGEDISNLLARRTQNMIRLQVTSLYFSCLLQEESRPSIEASLQKAMTLLSETEALARE
                     SLAMPSQIEQLRTMIKTSEAELAVNQEAQRVARAALLEAMGLSPSVPITIKKETPIVV
                     PDQEIEDQI"
     gene            complement(852..1361)
                     /locus_tag="PKH07_06130"
     CDS             complement(852..1361)
                     /locus_tag="PKH07_06130"
                     /inference="COORDINATES: protein motif:HMM:NF024988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNT34554.1"
                     /translation="MPKQETIERILSFADRLEGVDQSMLREALELIDLHQRLEHCIEN
                     MMPGSGLTARQVDILEILYHSLGQVVTSADLSEEVGLTRSAMTSALDSLERMGYVRRG
                     QHPSDRRMLAVSMTDAGENAIAEHLPIRYRHMYSIVSQLTPSERKTMRRVYNKLILML
                     NSFKEGNDR"
     gene            complement(1587..2396)
                     /locus_tag="PKH07_06135"
     CDS             complement(1587..2396)
                     /locus_tag="PKH07_06135"
                     /inference="COORDINATES: protein motif:HMM:NF024793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNT34555.1"
                     /translation="MNYLEFYHLSCLPFTNSPDARFFFESTQHKEAIIRIMHAIDNRL
                     GLAVLVGDVGTGKTTVSRRILETLDDDQYHASLLVAIHSDVSPMWILRKVAQQLGLAP
                     TGDEKGPLVETICARLFQLYEEGKKVVILVDEANMLRSPEVLEEFRGLLNLEMTDAKL
                     VTFVLIGLPELDNWLAQDKPLKQRTAVRFTLHALNPTATKSYITYRLTVAGRTEPLFT
                     EDAFKAIYEHSQGIPRLINTICDNALLEGALVKQEQLGLDLVKDVAIGLGL"
     gene            complement(2434..>3299)
                     /locus_tag="PKH07_06140"
     CDS             complement(2434..>3299)
                     /locus_tag="PKH07_06140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34556.1"
                     /translation="KRFRCSYSKCKGSRGGSLFELFRLAEELSEAQAAQRLADYFGIE
                     VHGSLEDEPSLPVEEPIVIPESAAVEEKEPELELLSLPSDVESEDELAALVEDAVQGI
                     AQDFVHSEEEIDAQAKQLDLTLSEEPPAPSVDVSDQAYQSQASESNWEVLCQAGMELF
                     EENRYSQAESKLAEAFKVAPAEQEKAQVALVLGKCYLEGRRPNEAAVLLESVLKNGGL
                     PGTLDKSLRKLLISVYEKKEDLAQVLSLMKDLARRHGEDPEITQKINQLEDALTPGAK
                     TPPKAKRISFL"
BASE COUNT          754 a          900 c          836 g          809 t
ORIGIN      
        1 gatttggtct tcaatctcct ggtccggtac cacgatcggg gtctcttttt tgatcgtgat
       61 tggcacactg ggagacaatc ccatcgcctc aagcaaggca gcacgagcca cacgttgcgc
      121 ctcttggttg acagccagtt ctgcttcgct ggtcttgatc atggttctca actgctctat
      181 ctggctgggc atcgcaagac tttcgcgtgc cagcgcctca gtctctgaca acagtgtcat
      241 cgccttctgc aggctcgcct ctatgctcgg gcgtgactct tcttgcagga gacaggagaa
      301 atagagagat gtgacctgaa ggcgaatcat gttctgagtg cgtctcgcta gaaggtttga
      361 tatatcctca cctttgacat aggcatcata gagaaaccag gtctggggag cgaagattgc
      421 ctgttgagca cgaagcgtgc cctcagtcac cgtctggtca gacataggga cgtatccccc
      481 acccgccttg accatccggt gtttcaagga cgacacggtg tcaaagcttg catcaagatg
      541 aggcaggaaa ttcgagaagg cgattttccg attcaggctc gccaacctct tgtctatttc
      601 tgaggccttg acgttcaggt tgttcctaag cgcgatctca atgcattgtt gcaacccgat
      661 cggttgttca gaggaaagtt cgagtgttga gcgttccgcc agctctcttg taaaagcctc
      721 agtgtgctcc tgcctcatga ggccaaaatc aatccggcga caagaggaca gagcgatacc
      781 gaagactgcc aacaacagga tcaaactcca cgttgaactc ttcagatgcc tcttgtcaga
      841 tgtctgtcgt ctcatctgtc gttcccttct ttgaatgagt tcagcataag gatcagcttg
      901 ttgtagacac gccgcatggt ctttcgctcc gaaggggtca gttggctgac aatgctgtac
      961 atgtgcctgt agcggatggg cagatgctcg gcaatggcat tctctccagc atccgtcatg
     1021 ctgacagcaa gcatccgtcg gtctgagggg tgctgtcccc gcctgacgta acccatccgt
     1081 tccaaggagt ccagcgcact ggtcatggct gaacgagtca acccaacctc ttccgagaga
     1141 tcggcggaag tgacgacctg cccaagactg tggtacagaa tctcaagaat atccacttgt
     1201 cgggcggtca atcccgaccc tggcatcatg ttctcgatgc agtgctcgag tctctggtgc
     1261 agatcgatca gttcgagggc ttctcgaagc atgctctggt caaccccctc aagtcggtcg
     1321 gcaaatgaga gtatacgttc aatggtctct tgctttggca tctgataact gacctctctc
     1381 aaaaagtaaa tacttcatgt tctgataata cgatatcgaa ttatttgatg tcaagttatt
     1441 tttttcagaa tccaaatccg cttcctgaga gtggctcgca gatggtggaa aaatcgtggc
     1501 aatacaagtt catgcagccg caaatcatga atatgaaaaa agatgggggg cagttcgtac
     1561 agccgaagga gcctgacttg gagccactac agcccaaggc cgatggcaac atccttgacg
     1621 agatcaagtc ccagctgctc ctgtttgaca agcgcacctt caagcagtgc attgtcgcag
     1681 atggtgttga ttagacgcgg tatgccttga gaatgttcgt agatggcctt gaaggcatct
     1741 tcggtgaaca gaggttcagt gcgacccgcc accgtcagtc ggtatgtaat gtaggacttt
     1801 gtcgcggtcg gattcagagc gtgcaaggta aaccgaaccg cggtacgctg cttgagaggc
     1861 ttgtcctggg caagccagtt gtcgagttcg ggaagaccaa tcagcacgaa ggtgaccagt
     1921 ttggcatcgg tcatttcgag attcagaagt ccccgaaact cctcaagaac ttctggggaa
     1981 cgaagcatat tggcctcatc aacgagaatg accactttct tcccttcttc gtagagttgg
     2041 aagagtcggg cgcagatcgt ctcgaccaaa ggtcccttct catctcccgt cggcgccagc
     2101 cccaactgct gcgccacttt tctcagaatc cacatgggac tcacatcgct gtggatggca
     2161 acaagaaggc tggcgtgata ttggtcgtcg tcaagagttt cgagaatccg tcgggaaacg
     2221 gtcgtcttcc ccgttccaac atctccgaca agcaccgcca ggccaaggcg attgtcaatg
     2281 gcgtgcatga tacgaatgat tgcttccttg tgctgtgtgc tttcaaagaa gaaacgcgcg
     2341 tcgggcgagt tggtaaaagg aaggcatgaa agatggtaaa actctaaata attcattcac
     2401 tggtcctgac tcaggccgat gcctctctca gcgctagaga aaactaatcc gcttggcttt
     2461 cggaggcgtc tttgctcccg gagtcaaggc atcttcaagt tggttgatct tctgggtgat
     2521 ttcaggatcc tctccgtgac gccgcgccag atccttcatc agagacagaa cctgagctaa
     2581 gtcttccttc ttctcataga ccgagatgag caacttacgc aacgatttgt ccagcgtacc
     2641 gggaagtccg ccattcttga gaaccgattc cagaagcacc gctgcttcat tcggtcgcct
     2701 gccttccaga tagcacttgc ccaagaccaa agccacctgg gctttctcct gttctgctgg
     2761 ggccaccttg aatgcttccg ccagtttcga ttccgcctgg ctgtagcgat tctcctcgaa
     2821 caattccatt cccgcctgac agagcacttc ccagttggat tccgaagcct gcgactgata
     2881 cgcctgatcg gagacgtcca ccgaaggggc cggcggttct tcggagagcg tcaaatccag
     2941 ttgctttgct tgagcgtcaa tctcttcttc cgagtgaacg aaatcctgag caatgccctg
     3001 aaccgcgtcc tcgacgagcg ccgcaagttc gtcttcactc tccacatcgg aaggcaacga
     3061 aagaagttcc agttccggct ccttctcctc aactgcagca gactcgggaa tcacaatcgg
     3121 ctcctcgact ggtagactgg gttcatcctc cagcgatcca tgaacctcaa tgccaaaata
     3181 gtctgccagc ctctgagcgg cttgcgcttc ggataactcc tctgccagac gaaacagttc
     3241 gaagagactg cccccccgag atcctttgca ttttgagtaa ctgcaacgaa atcgcttct
//
LOCUS       DAOQUC010000283         3292 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_33374_length_3292_cov_3.098239, whole genome shotgun sequence.
ACCESSION   DAOQUC010000283 DAOQUC010000000
VERSION     DAOQUC010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3292
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33374_length_3292_cov_3.098239"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..428)
                     /locus_tag="PKH07_06145"
     CDS             complement(<1..428)
                     /locus_tag="PKH07_06145"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNT34557.1"
                     /translation="MKVVQFASSSTWTGEMAHVFSLSRSLANRGAEITLAFRTIRKGK
                     GIPLPGEKATEFGFSSVLALNLDSGFYPFQMVSDLRKLIRHIDREGIQVLHTHRGQDH
                     WLAVVASKLSSRKPHVVRTRHVSVALRTHILNRWLFEQTD"
     gene            complement(425..2068)
                     /gene="lnt"
                     /locus_tag="PKH07_06150"
     CDS             complement(425..2068)
                     /gene="lnt"
                     /locus_tag="PKH07_06150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HNT34558.1"
                     /translation="MRDQKMNNPPEGDTSAPRRKRRSAKGIPSSPAGKVSVSHVCVAK
                     PQHFAKFMSLKGILLPVLTALLLLLGFPPFDLFLAPWFALVPFFLAVRHGAHWKNALK
                     GLLSGTLFFALLIWWLNYVTTIGYVLLVLYLSLYFAFASWFLGLFRSNALRALVLSPL
                     WVSLEFFRSSGIFGFSWGLLGHTQYRSLPTIQIASSTGVYGISALLAATSAALGYWFL
                     QRCEKRCRFRALHAVIVVVVLVHLFGLWALSQHPEGTPLKVGLLQGNVPQLLKWEPDM
                     EDPTIKLYTQMTHDALARGAELVVWPETAVPDVLDWKPDWENQFKQFCAKERFNLLFG
                     ALHHDVDAGRYFNSAYYMTPESASAEHGVQRYDKMRLVPFGEYVPFRNLLPFVQEVVE
                     SEGGGLFSGGVDLTVFQAGRAAFSTLICFESTLPSVARVLVQNGARALVVLTNDEWFG
                     RTPASLQHGLQSVFRAVENRVPVVRATNTGWTCSIDTRGRILATVPRFEEGILVDNMP
                     VPDRVGTVYTRWGDWFAWLCVVVVTTMAALTLKKQRVKR"
     gene            complement(2037..2279)
                     /gene="yacG"
                     /locus_tag="PKH07_06155"
     CDS             complement(2037..2279)
                     /gene="yacG"
                     /locus_tag="PKH07_06155"
                     /inference="COORDINATES: protein motif:HMM:NF015821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HNT34559.1"
                     /translation="MIQERSLPRAHKCPTCGKPVEPRKPQDPTPPEYPFCSARCKQID
                     LGRWFLGNYAISSKMQPEQEEIEPIEDERSEDEQSA"
     gene            complement(2276..2803)
                     /locus_tag="PKH07_06160"
     CDS             complement(2276..2803)
                     /locus_tag="PKH07_06160"
                     /EC_number="1.17.7.3"
                     /inference="COORDINATES: protein motif:HMM:NF016438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HNT34560.1"
                     /translation="MRTKSWEIAFHKEPCTASLCALLGEAIDRCKPRAIGVWAMQDDA
                     STERLLTMLRTILVSLNRCPVGLRLVSCPTCARCRVNLIAYAREVQQSLRERSGNLVV
                     AVMGCEVNGPGEARSSDVGIAFGRGAGLIFRKGENIRKVSADKAVQSLMDEVDLLLRV
                     DESQNSDMSKGLGVQ"
     gene            complement(3002..>3292)
                     /locus_tag="PKH07_06165"
     CDS             complement(3002..>3292)
                     /locus_tag="PKH07_06165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34561.1"
                     /translation="RSESVCAAVVIMDIRHAPTPLDKELMDWLQTFSVKIIPVLNKAD
                     KLSKSKQNERIREIRREWIWCDDKALITFSALNGAGKDQLESSIVQSIRRKD"
BASE COUNT          857 a          919 c          811 g          705 t
ORIGIN      
        1 tgatcggttt gctcaaaaag ccatcggttc agaatgtggg ttctcaaagc aaccgagaca
       61 tggcgagttc tgaccacatg aggttttcgg gaactgagtt ttgaggcgac aaccgcaagc
      121 caatggtcct gtcctcggtg tgtgtgaaga acttgaatac cttcacggtc aatgtgtcgg
      181 atcaactttc ggagatccga aaccatctga aagggataga agccggaatc cagattcaga
      241 gcaagcacac tgctgaatcc aaactcggtt gccttttccc ccggcagtgg gattcccttc
      301 ccttttcgaa tcgtccgaaa cgcaagcgtg atctccgcgc cccggttggc aaggcttctg
      361 ctcaagctga acacatgagc catctcacct gtccatgtgc tggacgatgc aaattgaaca
      421 accttcatct cttcaccctc tgctttttca gcgtcagcgc agccatggtc gtgacgacga
      481 caacacaaag ccaggcaaac cagtctcccc aacgagtgta aaccgttcca actcggtcgg
      541 gaacaggcat attatctaca agaattcctt cctcaaagcg aggaaccgtg gcaagtattc
      601 tccctcttgt atcaatggaa cacgtccatc ctgtgttggt cgcccgaacc actggaactc
      661 ggttttcaac ggctcgaaag acggactgaa gtccgtgctg aagacttgcc ggcgtgcggc
      721 caaaccattc atcattcgtc aaaacaacca aagcccgtgc accattttga accaagaccc
      781 gcgccaccga gggaagcgtg gactcaaagc agatcagcgt ggaaaaagca gctcggcctg
      841 cctgaaacac cgtcaaatca acaccaccag agaacaatcc accgccctcg ctttcgacca
      901 cttcctgcac aaacggcagc aagtttcgaa atggcacata ctctccaaag ggaacaagac
      961 gcatcttgtc atatctttgg actccatgct cggcagaagc actctctgga gtcatgtagt
     1021 aggcggagtt gaaatatcgt ccagcatcta catcatgatg aagcgcgcca aacagcagat
     1081 tgaacctttc tttggcgcaa aactgcttga attgattttc ccaatcaggt ttccaatcga
     1141 gcacatccgg aaccgctgtt tcaggccaga caaccagttc ggctcctcgg gcgagagcgt
     1201 cgtgcgtcat ttgagtgtag agtttgatcg ttggatcttc catgtcgggc tcccacttca
     1261 gaagttgcgg aacatttccc tgcagaagtc caaccttgag tggcgtccct tctgggtgct
     1321 gagacagagc ccagagcccg aaaagatgca ccaatacaac aactacaatg acagcgtgaa
     1381 gagcacgaaa gcgacagcgt ttctcacaac gctgtaaaaa ccaatacccc agagcggcag
     1441 aagtcgcagc aagcagtgca ctgattccat agactcccgt tgatgaggca atctggatcg
     1501 tcggtagact ccggtactga gtgtgcccaa gaagtcccca tgaaaagccg aaaatcccgg
     1561 acgatcggaa gaactccagc gatacccaga gtgggcttaa aacaagcgcc ctgagggcgt
     1621 tcgagcggaa gagaccgagg aaccaagagg cgaaagcgaa ataaaggcta aggtatagaa
     1681 ccagaagaac atacccaatc gtcgtcacat agttcagcca ccagatgagc agagcaaaga
     1741 aaagggttcc tgacagcagc cctttcaggg cattcttcca gtgtgccccg tgccgaacag
     1801 ccagaaagaa tggaacaagc gcaaaccagg gagcgagaaa gagatcaaac ggcggaaaac
     1861 caagcagcag gagcaacgcg gtcaacactg gcagcaggat gcctttgaga ctcataaact
     1921 tggcgaaatg ctgtggcttt gccacgcaca cgtgggagac agacactttg ccagcggggc
     1981 tcgaagggat tcccttcgcc gatcggcgct tgcgccgtgg tgccgaagtg tctccctcag
     2041 gcggattgtt catcttctga tctctcatcc tctatgggtt ctatttcctc ctgctccggt
     2101 tgcatcttcg aactaatggc atagttgccg agaaaccatc ttcccaaatc tatctgtttg
     2161 caccgagcgc tgcaaaaagg atactcaggc ggtgtcggat cttggggctt acgaggttcg
     2221 accggcttgc cacaggtggg gcacttgtga gcacgtggta acgaccgttc ctggatcatt
     2281 gtactccaag acctttcgac atatcgctgt tttggctctc atcaaccctc aacagcaggt
     2341 caacctcatc catcagagac tgtactgcct tatcggcaga gactttacgg atattctcgc
     2401 ccttgcgaaa gatgagtccc gctccgcgcc caaacgcaat cccaacatcc gaagacctgg
     2461 cttctccggg gccatttact tcgcagccca tgacagcaac gacgagattc ccggagcgct
     2521 cccgtagcga ttgctgaacc tcgcgagcat aggcaatcag attcacacga cagcgtgcac
     2581 aggtaggaca agaaaccaga cgcaatccaa ccggacaacg attcagactc accagaatcg
     2641 tcctcagcat ggtcagtagc ctctcggtgg aggcgtcatc ctgcatagcc caaacgccaa
     2701 tcgctctagg cttgcatcgg tcaatggctt cacccaataa tgcacacaag gatgcagtac
     2761 aaggctcttt gtgaaatgca atctcccagg atttcgttct catcaactcg tcggtcgaaa
     2821 gagaagcaag atcatcaagt gagcggattc ggaagacttc ttcagctccg ctctgtgagg
     2881 caatgtgatc cacaaggccg tccaccaagg cagaaagcct gttgccacgc tcctgggcgg
     2941 actggcaatc cgggacaatg agattgagaa aagttctgtc acgctcgtca aacatggctg
     3001 ttcagtcttt ccgtctgatg ctctggacaa tggacgactc gagctgatct ttgccagcac
     3061 cgttcaaagc ggaaaaagtg atcagcgcct tgtcgtcgca ccaaatccac tctctccgta
     3121 tttcccgaat gcgttcgttc tgcttgctct tggagagttt atccgccttg ttgagaacag
     3181 gtatgatttt gacactaaag gtttggagcc agtccatcag ctccttgtcg agtggtgtcg
     3241 gtgcatggcg gatatccatg atgacaaccg ctgcgcagac tgattcactc cg
//
LOCUS       DAOQUC010000284         3292 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_33376_length_3292_cov_2.916281, whole genome shotgun sequence.
ACCESSION   DAOQUC010000284 DAOQUC010000000
VERSION     DAOQUC010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3292
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33376_length_3292_cov_2.916281"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..648)
                     /locus_tag="PKH07_06170"
     CDS             complement(<1..648)
                     /locus_tag="PKH07_06170"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNT34562.1"
                     /translation="MGSTERTFAGLLIVLVFALSYGGCGGGDSSRCVLTGQILREGEL
                     DSEGIQVFLPGTTYQALTDAEGRFRMSQMEPGTYTLVAKDVGYISIREGGVVLKPGEE
                     RQLSPKTLHRAASTGNVGSVEGVFTLKEMEDHSGVLVALDSNAFSTLTDEYGKFILPD
                     IPQGDHKLSASKPGFEGVDQIPVTVFTGEKTVLSPIELPKFEEGTSSLSGSIKGLV"
     gene            complement(733..2454)
                     /gene="pilB"
                     /locus_tag="PKH07_06175"
     CDS             complement(733..2454)
                     /gene="pilB"
                     /locus_tag="PKH07_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012469711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HNT34563.1"
                     /translation="MRSSRTERSLGNFLVREGHITNEQLDMAIAKAVEVNKGLSEVLV
                     ELGLIDQENLTQALSRQHGIPYLPLADYDIDPSIIETVPEEDARRYKMIPVDRTEKTL
                     TVALADPGNILVLDELKLKTKMDIEPLLSLPKDIEQAIEKYYRKDHGDFEETMRLATQ
                     EVDMDVVPEASLEATESEDADAAPVIRLVNAIVSQAIEMRASDIHIEPQEHNLRLRYR
                     LDGVLQEKKPLPKQMQNAVISRLKIMSNLDISEKRHPQDGRFKARHNGNTVDFRVSML
                     PTVYGEKIVLRLLDKGNLSLDMAKLGFEPESLERFDRAIKRPYGIILVTGPTGSGKST
                     TLYSALSTINDPETNITTVEDPVEFQIQGINQVQAHLEIGFTFADALRSILRQDPDVV
                     MIGEIRDRETAEIAIKAALTGHLVLSTIHTNDAPSTPTRLIDMQVEPFLVTSALILVV
                     AQRLCRKICLECKEAYRPDADTVKQLGIDASLVQKMGLKEIVFYRGAGCAKCNGTGYK
                     GRMALYEVMEMDDYISSGIMKGMPPNELKKIARQRGMLTLRDSGIRKVLQGSTSVKEV
                     LSVTFEN"
     gene            2821..>3292
                     /locus_tag="PKH07_06180"
     CDS             2821..>3292
                     /locus_tag="PKH07_06180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34564.1"
                     /translation="MPVVRIASVRSVPYLCLGFVLLFSQGLCFGQTDSETASIPAAVT
                     QGLEVIQTWHDTPIVSATQTPLPPPSPTPTLKPPLNEINDWHYQLQSVNLTQLRDCAY
                     DLLVMDYADDDGNEYTSGQITGLSTQSNPKKVLAYLSIGAENYRFYWLPSWRTGN"
BASE COUNT          754 a          924 c          829 g          785 t
ORIGIN      
        1 aacgagtccc ttaatcgaac cagacaacga actggtgccc tcttcgaact tgggtagttc
       61 aatgggagaa agaactgtct tctctcccgt gaagacggtc accggaatct gatccactcc
      121 ttcaaagccc ggtttgctgg cagagagttt gtggtcgccc tggggaatat ctggaagaat
      181 gaacttgcca tactcatcgg tcagtgtcga gaaggcgttg ctgtccaatg ccaccaagac
      241 accggagtga tcttccatct ccttgagcgt gaacacccct tctaccgaac caacgttgcc
      301 agtcgaggcg gcacgatgca gtgtcttggg tgacaactgc ctttcctctc caggcttcag
      361 cacaacacca ccttcccgaa tcgagatgta gccaacatct tttgccacca gagtgtaagt
      421 gcccggctcc atctgtgaca tgcggaatcg cccctcagca tcggtcagag cctggtatgt
      481 tgttcccggg agaaagacct gtatcccttc gctgtccagc tcaccttcgc ggagaatctg
      541 ccccgtcaga acgcaacgcg acgagtcacc gccgccacat cccccgtaag agagagcgaa
      601 gacgagtacg atcagaagtc cagcaaatgt tctttcagta ctacccatgc aaatctccgg
      661 aacagtcctg tttgacttca attggattat tttcccagat ggggagactt gtcaaccgga
      721 cagaagaaaa gatcagtttt cgaaggtgac gctgagcact tccttgacgg aggtgctgcc
      781 ctgaaggact ttgcgaatgc cactgtcgcg aagagtgagc atcccacgct gccgcgcaat
      841 cttcttgagt tcgttgggtg gcattccctt catgatccca gaactgatgt aatcatccat
      901 ctccatgact tcgtacaacg ccattcgtcc cttgtatccg gttccattgc acttggcaca
      961 ccccgcgccc cgatagaaga caatctcttt caagcccatc ttctgaacta acgaggcgtc
     1021 aatacccagt tgcttgacgg tgtctgcatc aggtcggtat gcttctttgc actccagaca
     1081 gatttttcga cagagcctct gcgcaacaac cagaatcaat gcggatgtga ccagaaaagg
     1141 ttcgacctgc atgtcaataa gtcgagtggg ggtgctggga gcgtcgttgg tgtgaatcgt
     1201 tgaaagcacc aagtgtccgg taagggctgc cttgatggcg atctcagcgg tctcccgatc
     1261 tcgaatttct ccgatcatca ccacgtccgg atcttgtcgg agaatggagc gcagtgcatc
     1321 cgcaaaagtg aacccgatct ccagatgcgc ctgaacctgg ttgatcccct gaatttgaaa
     1381 ctcgacagga tcctcaacag tggtgatatt ggtttccgga tcgttgatgg ttgacaaagc
     1441 cgaatagagc gttgtggact tgccactacc ggtcggaccc gttacaagga tgatcccgta
     1501 gggcctcttg attgcgcgat cgaaacgctc caacgattcc ggctcaaatc ccaatttcgc
     1561 catatccaaa gagaggttgc ctttgtccag aagtcgaaga acaatcttct cgccatagac
     1621 cgtcggaagc atggacacac ggaagtccac agtgttgccg ttgtgtcgtg ccttgaaacg
     1681 accgtcctgg ggatgacgtt tctcggaaat gtccagattc gacatgatct tcagacgaga
     1741 gatgaccgca ttctgcatct gcttgggaag tggtttcttc tcctgaagaa cgccatccaa
     1801 acggtatcgc aaccgaaggt tgtgctcctg tggctcgata tgaatatccg aggcgcgcat
     1861 ctcaatggct tgggacacga tagcgttgac cagccgaatc acgggcgcgg cgtcagcgtc
     1921 ctcgctttcg gttgcctcaa ggcttgcctc aggcacaaca tccatgtcca cttcctgagt
     1981 ggccagacgc atcgtctctt caaaatcccc gtggtctttt cggtagtatt tctcgatggc
     2041 ctgctcgatg tccttcggaa gactcagcaa cggctcaatg tccatttttg tcttcaactt
     2101 cagctcatcg agcacaagaa tgtttcctgg atcggcaagc gcgaccgtaa gggtcttttc
     2161 ggtgcgatcc acagggatca tcttgtatcg tctggcatct tcttcgggaa cggtctcgat
     2221 aatggatggg tcaatgtcat agtccgccaa aggaaggtag ggaatgccat gctggcgact
     2281 cagcgcctgg gtcaggttct cctgatcgat caagcccagt tctacgagca cctcgctgag
     2341 ccccttgttg acttccacgg ccttggcaat ggccatatcc aactgctcgt tggtgatatg
     2401 gccttcccga acgagaaagt ttcccagtga acgctctgta cgactgctac gcatggcgac
     2461 cgcctcttct cttgctcaca accaaacacg ccgggcgaag tacgtgtacc acccagctga
     2521 atcgaatttt agcccgaacg gctttcgaat gcaactctaa gtacattcag gccaaggcaa
     2581 tcctatactt gacagccatc gtgtcaagaa gaatactact tcaggcgtaa cctgtccgtc
     2641 tttgtgagtt tctgctgact atgagtcggc agaaaggcgg agtcctcgga gcgtcgctgc
     2701 tgacagtagg cattccggct tcggcagcac tggcgaccga agcgcacttt gtgcaagaga
     2761 gactgacggg ttaccttcgg ttacgaccag cggttcgcag tgcgtcaaag gaggcaacaa
     2821 atgccagtcg tcagaattgc atcggttcgg tcggttccgt atttgtgtct tggatttgtg
     2881 ctgttgtttt cgcaaggtct gtgctttggg cagacggatt cagaaaccgc ttcgattccg
     2941 gcagcagtca cgcaaggctt ggaggtcatt caaacctggc acgatactcc catcgtctcc
     3001 gccacgcaga ctcctcttcc gccacccagc cccacaccga ccttgaaacc accactcaac
     3061 gagatcaacg actggcacta tcagcttcaa agcgtcaacc tgactcaact tcgcgactgt
     3121 gcctatgatc tcctggtgat ggactatgcc gacgacgacg gcaatgaata caccagcggg
     3181 cagattaccg gcctttcgac acagtccaat ccgaaaaagg ttctagcgta tctgagtatc
     3241 ggagcggaga actatcgttt ctactggctg cccagttgga gaactggcaa cc
//
LOCUS       DAOQUC010000285         3291 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_33393_length_3291_cov_3.424907, whole genome shotgun sequence.
ACCESSION   DAOQUC010000285 DAOQUC010000000
VERSION     DAOQUC010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3291
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33393_length_3291_cov_3.424907"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..428)
                     /locus_tag="PKH07_06185"
     CDS             complement(<1..428)
                     /locus_tag="PKH07_06185"
                     /inference="COORDINATES: protein motif:HMM:NF019156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34565.1"
                     /translation="MNILGRTLSKIFGTQQERDMRRYTPVVKQILEEIQPSLQSLSDD
                     QLRARTEELRQRYRAEVSESLLEMLGNLDTEEISVPHLRAILTEFKRAESSEERLELF
                     QELPRPFFPKHLPGPDRIVSEELHKKYTKLRFRVLYGLLP"
     gene            complement(425..3070)
                     /gene="alaS"
                     /locus_tag="PKH07_06190"
     CDS             complement(425..3070)
                     /gene="alaS"
                     /locus_tag="PKH07_06190"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HNT34566.1"
                     /translation="MMTGHEIRTAYERFFVERGHIAMPSDSLVPKTDPTLLFTSAGMV
                     QFKPYFLGQKGDELRRACSCQKCLRTSDLENVGHTARHHTFFEMLGNFSFGDYFKQDA
                     IVWAWQFMTEVLKLDKAKLWITIFENDDEAEKLWQSEAGVAADRIIRMGEDSNFWKMG
                     DTGPCGPCSEIHIDQGEEIGCGRPDCNVECDCDRFLELWNLVFTQFDRQADGTLEPLP
                     RKNIDTGMGLERIAAVMQNGRSNYDSDLFQPMFDRIQDLSGVRFGESRRTDIAMRILA
                     DHARAVLFALTDGITPSNTGRGYVIKRILRRALRQGRILGLKFPFFASVMDSVVSVMR
                     PAFSELTSTNINAANRIVEMEEESFGSILERGGELLYSRMDELRAKGESVLSGREAFK
                     LYDTYGFPFEQTLEILDEEGFSCSREDFEAKMKQQRERARQAWKGTGATDRMALDAAG
                     DLQATEFVGYTDECCEANILAIWDRHKRCTEASQGQKCEIVLDRTPFYAESGGQVGDG
                     GTLSAKGVRFVVQDTQKTAAGIVVHLGEVAEGTLLVNQDVMAEIDHDKRGRIRRNHTS
                     THLLHAALRHVVGSEAKQSGSKVDESGFRFDFACPRSLSKSELTDIEQMVNGWICGNH
                     PVQTRVVSLQDALAQGAMALFDEKYEDKVRLLEIADISKELCGGTHASRTGDIGSFRI
                     VSEGSVSAGVRRLEALTGLSAWAHAQSREAVLDDLCELLKSPCSDLSERVRQIQEQTR
                     NLEKQLEKELAQRAVSSAAELINQGEAIMVGDREVFLIRREIAGCTADQLNQMLDILR
                     DKAPEGVYVLGTQVDGKAVLVCGVSPSLTKVFPAGKIIKAAAAITGGGGGGRPDRAQA
                     GGKDGDKVPDALSKIPEILAEVQSH"
BASE COUNT          729 a          964 c          805 g          793 t
ORIGIN      
        1 tctggaagca ggccgtacaa gacacggaaa cggagtttcg tgtatttctt gtggagctct
       61 tccgagacaa ttcgatcagg tccgggaaga tgcttcggga agaatggtcg cggaagttct
      121 tgaaacagtt ccaaacgctc ctcggaactc tccgccctct tgaattcagt gagaatcgcg
      181 cgaagatggg gaacgctgat ctcttcagtg tccagattcc caagcatttc cagaagcgat
      241 tcgctgacct ccgcccgata tcgctgacgc aactcttcgg tcctggcgcg cagttgatcg
      301 tcggagaggg actgcagact gggctggatc tcctcaagta tctgcttcac cacaggcgtg
      361 tagcgacgca tatcacgctc ttgctgcgtt ccgaatatct ttgagagcgt tctgccaagt
      421 atgttcaatg gctttgtacc tcagccagta tctcgggaat cttcgaaagt gcatccggta
      481 ctttgtctcc gtctttgcca ccggcctgag ccctgtcagg gcgtccgcca cccccaccac
      541 ccgtgatggc agcagcggct ttgatgatct tgcccgcagg aaagacctta gtcagagacg
      601 gactgactcc acatactaga acggctttcc cgtccacttg ggtacccaga acatagactc
      661 cttcgggagc tttgtcgcgc agaatatcca gcatctgatt cagctggtcg gcagtacaac
      721 ccgcgatttc cctgcgaatg agaaagactt ctctgtcacc gaccatgatt gcttcacctt
      781 ggttgatcaa ctctgctgcg ctcgatactg ccctttgcgc cagttccttc tccagttgtt
      841 tctccaagtt ccgcgtctgt tcctgaatct gccgaactcg ttcgctcaag tcgctgcagg
      901 gactcttcag cagttcacac agatcgtcca agaccgcctc acgtgactga gcatgcgccc
      961 aggccgacag ccctgtcaac gcctcaagac gccggactcc agctgagaca gagccctctg
     1021 agactatccg aaaagagccg atatctccgg ttcgagaagc atgtgttccg ccacacaact
     1081 ccttgctgat gtctgcgatc tcaagcaggc gcaccttgtc ctcgtacttc tcgtcaaaga
     1141 gagccatcgc cccctgagca agtgcgtcct gaagtgatac aactcgcgtc tgcacaggat
     1201 ggttgccaca aatccatcca ttgaccatct gctcgatatc cgtcagttcc gacttgctga
     1261 gcgaccgagg acaggcaaaa tcaaaccgaa agccagactc atccaccttg gagccggact
     1321 gcttggcttc cgacccgacg acatgccgaa gcgctgcatg aagaagatgc gtagatgtgt
     1381 gattgcgtcg aatgcgtcca cgcttgtcat ggtcaatctc tgccattaca tcctggttaa
     1441 ccaacagcgt tccttccgcc acttccccca ggtgcacaac aatgcccgcc gccgtcttct
     1501 gcgtgtcttg caccacgaaa cgaacacctt tagcactcag cgttccacca tctccaacct
     1561 gccctcccga ctccgcgtag aaaggagtcc gatcgagaac aatctcacat ttctgccctt
     1621 gggaagcctc agtacatctc ttgtgccggt cccagatggc aaggatattc gcctcacagc
     1681 actcatccgt atagccgaca aactcagtcg cctgcaggtc tcccgctgca tccagagcca
     1741 tgcggtctgt cgcacccgtt cctttccagg cttggcgggc gcgttcacgc tgttgcttca
     1801 tcttcgcctc aaaatcctca cgtgaacagg agaatccctc ttcgtcaagt atttccaggg
     1861 tctgctcaaa ggggaatcca taggtgtcat agagtttgaa tgcttctcgt cctgacagca
     1921 cactttcgcc cttggctcga agctcatcca ttcgggaata gagcagttcc cctccccgct
     1981 caagaatgga accaaaactc tcttcttcca tctcaacaat tcggttggca gcgtttatgt
     2041 ttgtagatgt caattctgaa aacgccggtc tcataacgct gacgactgag tccatgactg
     2101 atgcaaagaa tggaaacttc aaaccaagaa ttcgtccctg cctgagcgca cgacgcaaaa
     2161 tcctcttgat gacatagccg cgtccggtgt tgctcggcgt gatgccatcc gtcagtgcga
     2221 acagaacggc tctggcgtgg tcagcgagaa ttctcatggc aatgtccgtt ctgcggctct
     2281 ctccaaatcg gacacctgaa agatcctgaa ttcgatcgaa catcggctga aacagatcac
     2341 tgtcgtaatt gctccgtccg ttctgcatga ctgcggcgat gcgctccaga cccatgccgg
     2401 tgtctatgtt ctttctcggc agaggctcca aagtgccgtc ggcttggcgg tcaaactggg
     2461 taaacacaag gttccacagt tcaaggaacc ggtcgcagtc gcattccaca ttgcagtctg
     2521 gacgtccgca gccgatctct tctccttgat cgatgtgtat ctccgaacat ggaccgcacg
     2581 gtccggtgtc acccatcttc cagaaattgc tgtcttcccc catgcggata atgcgatcgg
     2641 cggcaactcc ggcttccgac tgccagagtt tctccgcctc atcgtcattt tcaaagatgg
     2701 tgatccagag tttggctttg tcgagtttga gtacctcggt catgaattgc caagcccaga
     2761 caatggcgtc ttgtttgaag taatcaccaa aagaaaaatt gcccagcatc tcaaaaaagg
     2821 tgtgatgtct ggctgtgtgt ccgacatttt ccaggtcaga ggttcggaga cacttctggc
     2881 aggaacacgc gcgacgcagt tcatcgcctt tctgcccaag aaagtagggc ttgaactgca
     2941 ccataccggc gctggtgaaa aggagcgtcg gatcggtctt gggaaccagg gagtcgcttg
     3001 gcatagcgat gtgccctcgt tccacaaaga atctctcata ggctgtgcga atctcgtgac
     3061 cagtcatcat tctcgtcttt cctcattttt ctcatcggag aaagtatgca caaaggcagc
     3121 ctctgccctt ggctgcaaaa gtggtaaaac tgtagcacag agaaacgtgt tgttccactt
     3181 gagcaaacaa agactgtggt ggcgaagcga tgctctttcc tgtgcaacgt attggatcag
     3241 ttacggaggg ggtggaggta tgcttcggtt gcagaggctc tgcgacgctg c
//
LOCUS       DAOQUC010000286         3291 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_33396_length_3291_cov_3.121755, whole genome shotgun sequence.
ACCESSION   DAOQUC010000286 DAOQUC010000000
VERSION     DAOQUC010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3291
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33396_length_3291_cov_3.121755"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..375)
                     /locus_tag="PKH07_06195"
     CDS             complement(<1..375)
                     /locus_tag="PKH07_06195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34567.1"
                     /translation="MYRNRSFLPFLIVMVFPLMTPCVQAALTNGNFEYASTTGWATGA
                     GTYLITAGNRPGGSGMFYLQMVGIGGGTTISQAEFALQQGETWRVTGWIRNDTGSANM
                     NFGWYTSSLNPIATVSVTSTSWR"
     gene            complement(388..1572)
                     /locus_tag="PKH07_06200"
     CDS             complement(388..1572)
                     /locus_tag="PKH07_06200"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNT34568.1"
                     /translation="MNASEEIRERPKDPSIAVMVGLTVLVIWGCVTVLTMLQFVHEIN
                     LKMAINIHDAAEYYLRQADQASAQGDNEKAKRAQEQAHLYYKEMYRRFDRSMKLYPLE
                     METLYILGRYCIDNDNPSKGVEVLVRDLYLNPNYKWGHNNLGVCYDRLGNTEMARESY
                     YRALMVDRFQVYAHFNLGIGFLKEGKVTRAIPEFVATIESDPNKDSAYRYLGVCYKEL
                     GEYQHALETFEKYTDLLRSTKRRQDQPLDPDELQKELRLFYAEQMKLARLTEDVTAEA
                     RILKHAVDLEPGNVSTRKMLITTLQKMGDMHSVAAQIRAMTEYAPKDPHTWYNLALVE
                     ASNGRDATVVLDVVRKAAILGGPDVLERIRKERLFESYREDPAFREFLDSIPVLSATD
                     EL"
     gene            complement(1589..3091)
                     /locus_tag="PKH07_06205"
     CDS             complement(1589..3091)
                     /locus_tag="PKH07_06205"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HNT34569.1"
                     /translation="MFQLKRILWSGMYLMVVVFLVAATVNVSAQQVKAEPGKYRAFWV
                     NAFQPDMHTPQGVARAVKVARDYNFNTIIAEVRLQCDAFYNSTVEPKSPLVQPPDYDP
                     LKDMCAQAHDTTDGKQRLEVHAWIVTYRANLNMNNVPIGTGTPAHVLTQHPTWINQNF
                     KGETVSGNKNFLDPGVPGVIDHTVAVVKDIVQNYDVDGIHYDYIRYPEEDTSGACDWG
                     YNPISVNRFNRIYDRKGSPETGDPDWENFRRQQILDLLRKTYVEAKRIKPTIKMSAST
                     VNWGPCPDDFKQSAPYKRTLQDWVSFMKEGVLDINFLMNYKREHDAGQAADYRAWSKL
                     VASSKSGRHGVVGQGAYLNDPQGTVAQMKFDLSLPEIDGTALYCYSEARREADTKIVP
                     DPLIFDPIKNEIFTTPVDPPVAEWIVNPTKGICAGTVKVGKEPADRAIVTLSPGGKQT
                     HTDGTGFYAFMDVEPGDYQLKAKPLAGKETSEKTQCAKGQIAIKDITCKP"
BASE COUNT          737 a          863 c          861 g          830 t
ORIGIN      
        1 acgccagctg gttgaagtga ccgagacggt tgcaatgggg ttcagagagg atgtatacca
       61 gccgaaattc atgtttgcag agccggtatc attgcgaatc catccagtca cacgccaagt
      121 ttcgccttgc tggagtgcga actccgcctg ggagatggtg gttccccctc caatgccaac
      181 catctgaagg taaaacattc ctgagccgcc cggtctattg ccggcggtaa tcagataagt
      241 tccagctcct gttgcccacc cagtggtgga agcatactcg aagttgccat tggtgagggc
      301 agcttgaaca cacggggtca tcaaaggaaa caccataacg atcaggaaag gcaagaacga
      361 tctgttgcga tacatgatag acactcctca caactcatct gttgctgaca ggacagggat
      421 gctgtcaaga aattctcgaa atgcaggatc ctctcgatac gattcgaaca gtctctcttt
      481 ccgtattctt tcgagcacat ccggaccacc caggatggca gcttttcgca cgacatccaa
      541 gaccaccgtt gcatcccgac cattggatgc ttccaccaag gcgagattgt accaggtgtg
      601 cggatctttg ggtgcgtatt cggtcatggc tcggatttga gccgccacgc tgtgcatgtc
      661 gcccatcttt tgaagcgtcg tgattagcat tttgcgggtg ctgacgttgc cgggctccag
      721 atcaacggcg tgcttcagaa ttctcgcttc ggcggtcaca tcctcggtca atcgggcaag
      781 cttcatctgt tccgcataga acaggcggag ttccttctgc agctcgtcgg gatccagtgg
      841 ttgatcctgg cgacgtttgg ttgagcgcag caagtccgta tacttctcga atgtctccag
      901 tgcgtgttgg tattctccaa gttccttgta acacacgccc agataacggt aggcgctatc
      961 cttgttggga tcggactcga tcgttgcaac aaattcaggg atcgcccgcg tcaccttgcc
     1021 ctctttgaga aaaccgatgc caaggttgaa atgggcatag acctggaacc gatccaccat
     1081 cagagcgcgg tagtaactct cgcgtgccat ttccgtgttt cccaggcgat cgtagcagac
     1141 tcccaggttg ttgtgtcccc atttgtagtt ggggttgaga tagagatcac gaacgagcac
     1201 ctccacgcct tttgaggggt tgtcattgtc aatgcagtaa cgcccgagaa tatagagcgt
     1261 ctccatctca agcggataga gtttcatgga acggtcgaaa cggcggtaca tctctttgta
     1321 gtagagatgt gcctgctcct gagctctctt ggctttctca ttgtctccct gtgctgatgc
     1381 ctgatctgcc tgtcgcagat agtactcagc tgcatcgtga atgttgatgg ccatcttcag
     1441 attgatctca tgcacaaact gaagcatggt taagaccgtg acacaacccc agatcacaag
     1501 aaccgtcagc cctaccatta cagcaatgga tgggtctttc ggccgctctc gtatttcctc
     1561 agaagcattc atgaaagaac ctttctcatc agggcttgca agtgatgtcc ttgatggcaa
     1621 tctgcccctt ggcgcattga gtcttttccg aagtctcctt gcccgccagg ggttttgctt
     1681 tcaactgata atctccaggt tcaacgtcca tgaaggcata gaatcctgtc ccatccgtat
     1741 gggtctgttt tccaccgggg ctgagcgtca cgatggcgcg gtcggcgggt tccttgccta
     1801 ccttgaccgt gccggcacag attcctttcg ttggattcac aatccattca gcaacagggg
     1861 gatcaaccgg tgtggtgaaa atctcattct taatgggatc aaaaatcaag gggtcaggaa
     1921 caatcttcgt gtccgcttct cttcgggctt cggaatagca gtatagtgcc gttccgtcaa
     1981 tctcaggcag agaaagatcg aacttcatct gtgccactgt gccttgggga tcattgagat
     2041 aagcaccctg acccacgact ccgtggcgac ccgatttgct ggacgcgacc agctttgacc
     2101 aagcccgata gtcggcagcc tgaccggcat catgttcacg cttgtagttc atcaggaagt
     2161 tgatgtccaa aacaccttcc ttcatgaaag aaacccagtc ctgaagcgtc cgcttgtagg
     2221 gtgccgattg cttgaagtca tcaggacagg gtccccagtt gacggtggac gcagacatct
     2281 tgatggtggg tttgatgcgc ttggcttcga catacgtctt gcgaagcaga tcaaggatct
     2341 gttggcgtcg gaaattctcc caatcggggt cgccggtttc ggggctgcct tttcgatcgt
     2401 agatgcggtt aaagcggttg accgaaatcg gattgtaacc ccagtcgcag gctccggatg
     2461 tgtcttcctc tggatagcgg atgtagtcgt agtgaatgcc gtccacatca tagttttgga
     2521 caatgtcttt cacaacggcg accgtgtgat cgatgacacc tggaacgcca ggatccagga
     2581 agttcttatt ccccgacaca gtctcaccct tgaaattctg gttgatccag gtcgggtgtt
     2641 gcgtcagcac atgtgccgga gtgcctgttc caatgggaac gttgttcatg ttcaggtttg
     2701 cccgataggt gacaatccac gcgtgaactt ccaagcgctg cttgccgtct gttgtgtcat
     2761 gtgcctgcgc gcacatatcc ttcagaggat catagtccgg tggctgtacc aagggcgatt
     2821 tcggctccac cgttgagttg tagaaggcgt cgcactgaag tcggacttca gcaatgattg
     2881 tgttgaaatt gtaatctcgt gccaccttga ccgcacgagc aactccttga ggagtatgca
     2941 tgtctggttg aaaagcattg acccaaaagg cacgatactt gcccggttcg gctttcacct
     3001 gctgcgccga gacattcacc gttgcagcga caagaaagac caccaccatc aggtacattc
     3061 ctgaccatag aatacgcttc aattgaaaca tccagataac ctccgatttc ttctgcgtct
     3121 ggtcttttct gacctttgaa acagagcata tcacgaaacc gagaaaggcc tcaaggaaat
     3181 ggaggaaacg gacgtatggg ctcagttatg caggtgatac aggcaaggta tgcttcggaa
     3241 gcaaagcgga gtcccttcgg ctgcgctcag ggcaggcttc ggagcaacgc t
//
LOCUS       DAOQUC010000287         3289 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_33417_length_3289_cov_4.032468, whole genome shotgun sequence.
ACCESSION   DAOQUC010000287 DAOQUC010000000
VERSION     DAOQUC010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3289
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33417_length_3289_cov_4.032468"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /locus_tag="PKH07_06210"
     CDS             complement(<1..398)
                     /locus_tag="PKH07_06210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HNT34570.1"
                     /translation="MQIPNKTLRSGLVLLLLLFVGCQTLPTSDVPTAGQSRFSESQYV
                     EYIVGNLPVIISAPHGGRLAPPNIPDRARGTFSTDVDTDLLAREILEAFHKQTGKFPH
                     MIICHVKRIKVDCNRNLQDAALGDPGAEKV"
     gene            complement(710..1681)
                     /locus_tag="PKH07_06215"
     CDS             complement(710..1681)
                     /locus_tag="PKH07_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014246908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNT34571.1"
                     /translation="MFTNTMAQQISPTPEQRKRTPRLAIAALLFGATNMAIGSILVRI
                     SEVGPTASAFWRVALACPLLALTAQITKPRNVEGDLSDTGREKRICILAGLFFAGDLG
                     FWHYSLVWTSITNATLLTNLSPIFVVIGGWLVFRQRVSKMFLAGLVLAVGGMSALMRA
                     SFHVDSQHLVGDGMGLVSAVFYGGYLLSIKRARRRFSAVRTLAWSSLGTALVLLPATL
                     FLGEVWLPRTTRGWSIVLSLSLVAQMGGQGMVAFALAHLPAAFSSVTLLFQPAVAAVL
                     AWWLLREPVGSGQILGAMATLAGIWLARRGSDLNASGSTVRHSDSAR"
     gene            1899..>3289
                     /locus_tag="PKH07_06220"
     CDS             1899..>3289
                     /locus_tag="PKH07_06220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34572.1"
                     /translation="MRNALVILVLLGACLVSPVQGEAALELLGQTYRSDVDFPQHWPF
                     WGTPRALGGTAHVFVRNIGSDRITIGDVFLEGISLKSAIALSSQKKKGFLYASSIHFS
                     TLNAADMQKLMNAGEPIWWKSTPPKLEPGTTAEVIIRLRSVPRVQTFRVGVYTDTQTL
                     NADIPVVPIHPRIESISFSAARDKVYLYFKHPEGAAQELRHVFMDGRNVTAQTQIAYD
                     PNVSIVPAVLALAEPLVEGSFHCYWGEYADSTRASAGLRTYSGEMWYGLCGARPGSET
                     DFDLARRYLRDIHDHRINVQMPTLASSAVQSFLKTEEGQDLCEELGIRLMPNVPGKWN
                     TRDPVAFYLVDEPDVGEYSLVNLEEHQKAGTLAQNLFTYSEPFRETDPETPHLVNLDM
                     TLKPFNWYVYGLVPDVFCADPYYQNRLTTAYWSKPAEISRYQKATYITAVGELCQSAC
                     APNPLFLLLQSTV"
BASE COUNT          812 a          914 c          849 g          714 t
ORIGIN      
        1 cagacctttt cggcacccgg gtcaccgagt gcagcatcct ggaggttgcg gttacagtcc
       61 actttgatcc gtttcacatg acagatgatc atgtgcggga acttgcccgt ctgtttgtga
      121 aaagcttcaa gaatctcacg ggcgagcaaa tcggtgtcca catctgtcga aaaagtgcca
      181 cgcgcacgat cggggatgtt aggcggagca agtctgcccc cgtgaggcgc tgagatgatc
      241 acgggaagat tcccaacgat gtactccaca tattggctct cactgaatcg gctctgccct
      301 gctgtaggca cgtcgctcgt cggcaacgtc tgacagccaa cgaacaggag aagcaacaac
      361 acgagaccag accgcagtgt tttgtttggt atttgcatct ttgattccca cttttgatct
      421 gttgaaactc caggtgaagc gttcccaaac ttttcaaggc aaagcatcgt gaaaactcca
      481 acccgtactt tcaagagcaa agtattgcat cttctacaga gatttcaagg aaagatgttt
      541 gtctgctccc agcgtaaaga ggtgatgaga ctctccggct gaattctgtc cgagagagcg
      601 tattcgtgcc aacacggaaa cacggtgaca aacagaactc ggtgtcgcat ctgcactgga
      661 tagtattgca gcgcgatatt ccgtgtggct atcccacggt tctctcttct caacgggcag
      721 aatccgaatg tctgaccgtc gaaccactgg cattcagatc gctgccccgt ctcgccagcc
      781 atatccccgc gagagttgcc atcgcgccga gaatctgtcc ggagccgaca ggctcccgga
      841 gcagccacca ggcgagaacg gccgccacag cgggttgaaa cagaagcgta acggaagaga
      901 aagcagcagg cagatgagcc agcgcgaatg ccaccatacc ttgccctccc atctgtgcaa
      961 cgagtgacaa tgagagaaca atagaccaac cgcgcgtggt tcgaggcagc cagacctcac
     1021 ccagaaagag tgtcgccggc agcagaacaa gtgccgttcc cagactgctc catgccaagg
     1081 tacgaacggc ggaaaatcgc ctgcgagcac gcttgatcga aagcagatat cctccgtaga
     1141 aaacggcaga caccagaccc ataccatcgc ccacaagatg ctgtgagtca acatggaaac
     1201 tggcacgcat caacgcactc atgcctccga ctgccaagac caagcccgcg agaaacatct
     1261 tgctgacacg ctgccggaag accagccatc ctccaatgac gacaaagatc ggagaaagat
     1321 tggtcagcag cgtcgcattg gtgatcgagg tccacacaag cgagtaatgc cagaaaccca
     1381 gatctcccgc gaagaacaat cctgccagga tacagatccg cttctctctt cccgtgtcac
     1441 tcaaatcccc ctcgacgttt cgaggctttg tgatctgggc agtcaacgcg agcaaaggac
     1501 aggctaatgc aacacgccaa aaggcagagg ccgtgggtcc aacttcgctg atacgaacga
     1561 ggatcgaacc gatagccatg ttcgttgcgc caaaaagcag cgccgctatg gcaagacgag
     1621 gcgttctctt tcgttgctct ggagtcggac tgatctgttg tgccatcgtg ttcgtgaaca
     1681 actcgcttct gattctgcaa gtgcggaaac gggcagagcc ggaaaccttt tgtacctgct
     1741 atctatttgg aaagaggcgt attgtcaagc aacgggtgac atgcgcacac caacttgcaa
     1801 ttcgccagca cgatattttg taccaacgtc tggactttct gcgctaccat actttgtgag
     1861 tgaatgtatt ggccttcaat ctttcggaga cattggcaat gagaaatgct ttggtgattc
     1921 ttgttctgtt gggtgcctgc cttgtgagtc ctgttcaagg tgaagccgct ctggagcttc
     1981 tgggtcagac ctatcgaagc gacgttgatt tccctcaaca ctggccattc tggggaacgc
     2041 ctcgcgccct tggaggtacc gcacatgttt ttgtcaggaa tatcggctct gaccggatta
     2101 cgatagggga tgtctttctt gaaggaatca gcctcaaatc tgccattgct ctatcgtccc
     2161 agaagaagaa gggatttctg tatgcctcaa gcatccactt cagcacactc aatgctgccg
     2221 acatgcagaa gctgatgaat gcgggagaac cgatatggtg gaagtccact ccgccaaagt
     2281 tggagccggg cacaacggcc gaagtcatca tccgattgcg aagcgtgcca cgagtccaga
     2341 cgtttcgtgt cggagtgtat accgacacgc agacgctgaa cgccgacatt cccgtcgttc
     2401 caattcatcc tcggatcgaa agcatctcct tctccgcagc acgagacaag gtctatctct
     2461 atttcaaaca tcctgaagga gcagcacagg aactccgcca cgtgtttatg gacggtcgga
     2521 atgtcacagc acagacacaa atcgcgtatg accccaatgt gagcatcgtc cccgcagtgc
     2581 tggcattggc cgaacccctc gtggagggat cttttcactg ctactggggt gaatatgcag
     2641 acagcacaag ggcaagtgcc ggtctgagaa cgtacagcgg agagatgtgg tacgggcttt
     2701 gtggtgccag gcctggaagt gagacggact tcgacttggc tcgccgctac ctgcgagata
     2761 tccatgatca ccggatcaac gttcagatgc ccactcttgc ttcgtcagct gttcagtcat
     2821 tcctgaagac cgaagaaggc caggatctct gtgaagagct gggcattcga ctgatgccca
     2881 atgtcccggg caagtggaac acaagagacc ctgtggcgtt ctaccttgtt gacgaaccgg
     2941 atgtgggtga gtacagcctg gtgaaccttg aggagcacca aaaggcagga acactggcac
     3001 agaatctctt tacttactcc gaaccctttc gcgaaaccga tcctgagaca cctcatctgg
     3061 taaacctgga tatgacgctc aaaccgttca actggtatgt ctacggtctc gtgcctgatg
     3121 tcttctgtgc cgatccctac tatcagaatc gcctgacaac cgcctactgg tcgaaacctg
     3181 ctgagatctc ccgttaccag aaggcaacat acatcaccgc cgtaggtgaa ctgtgccagt
     3241 ccgcgtgcgc tccaaatccg ttgtttcttc tgctgcagtc aaccgtctg
//
LOCUS       DAOQUC010000288         3285 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_33487_length_3285_cov_2.950155, whole genome shotgun sequence.
ACCESSION   DAOQUC010000288 DAOQUC010000000
VERSION     DAOQUC010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3285
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33487_length_3285_cov_2.950155"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1060)
                     /gene="der"
                     /locus_tag="PKH07_06225"
     CDS             complement(<1..1060)
                     /gene="der"
                     /locus_tag="PKH07_06225"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HNT34573.1"
                     /translation="MGKPIVAIVGRPNVGKSSLFNRLVGRRQAIVLDQPGITRDRVYG
                     DVSWGNRVFALIDTGGYVSDAADVLTQSMLANIQQAVQESDLVVFLGDAVDGPTADEY
                     AIAAYLRKTGRPVIVAVNKVDNPNREDLLHAFYGLGFEPVIGISALHGLETGDLLDHI
                     VSLLPRHRDESLQEHKDAIPVAIIGRPNVGKSTLLNSLLGKQRALVDSTPGTTRDPVD
                     VLKEHDGKKYLFVDTAGIRRKGKIERGAEWVSVLLAQQSLDRCKVALLVLDAQEGPVE
                     GDAHVFGLAQKENKACIILANKWDTLEKDDKTSGEMINKIRDTIKFLPYAPIMMISGL
                     TGQRVSKIYDLIDTVYASY"
     gene            1340..>3285
                     /locus_tag="PKH07_06230"
     CDS             1340..>3285
                     /locus_tag="PKH07_06230"
                     /inference="COORDINATES: protein motif:HMM:NF024516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3971 domain-containing protein"
                     /protein_id="HNT34574.1"
                     /translation="MTKAIFVRRGFHQFQPSRQQPRPWKPIVVLAFILLLVVGLMPVP
                     SSRLEKRLQESFQEAFGGAASFSSCSIRIRDFRLYTDETQQQLLLECSRVRVGYNPVF
                     YPLTGAYGLYLSKPRLIVPNAVDGQFQTPKSLSNAFKGDSEGTSNVPSFIRVDDARIE
                     YTCMVTSVPVLGEDIDIAITRLRSEKPKALECRGRALWAGQGLSFTSEGTVNLLEKSA
                     SLSGIIRPATGTEISVNLLGYKTAGEVVSSATEQGAGEDKDSVPTGANRTQFSAKSVP
                     WTLNASILESLLSLDVALTPQEGFLTLSETLKPSLKQGTGRFKYDRAMGKFSVEDFTI
                     SLDNYHATANGEFGLTGYKPFRLKFSSQDMPSEVIRTLLAQRQPELPFVLVSGILGFQ
                     IESSGLMTNPAGTFSQGRLDCQGCTFEHVATGFRITSVSGGIDFSPEEAVFRQLEARY
                     TDRKFSIDGSVRGDPYIWDDPVVDVKAQGEIDLMQTGKFIHALIPAFVGDTKISGNAL
                     LDIAATGRVSDPNSLTFDGSVDLQNVSVSGGLFPVPFNHVSGQVVFRDNTMTLNDLQA
                     STGKTTVALQGTYKRGEQSWREGTVDLKLVGVAEAEEIFPVLQRSMPKYLEGYRVAGP
                     LEYNLSVSGLLSSTAERTWSGNAL"
BASE COUNT          760 a          857 c          888 g          780 t
ORIGIN      
        1 ggtaactggc atatacggtg tcaatcaagt cgtagatctt cgagacgcgt tgccctgtca
       61 accctgaaat catcatgatc ggcgcgtaag ggagaaactt tattgtgtcc cgaatcttgt
      121 tgatcatttc tccggacgtc ttgtcatctt tttccagcgt atcccatttg ttcgccagga
      181 tgatacaagc cttattttcc ttctgcgcga gaccaaagac atgcgcatcc ccttcgaccg
      241 gtccttcttg ggcgtccagc acgagcaacg cgaccttgca gcgatccaac gactgctgcg
      301 ccagcagaac gctgacccac tccgccccgc gctcgatctt tccttttcgc ctgatccctg
      361 ccgtatccac aaagaggtac tttttcccat cgtgctcctt caagacgtcc accggatccc
      421 gcgtcgtgcc gggagttgaa tcaacgagag cgcgctgttt gcccaacagg ctgttgagca
      481 gcgtagactt gccgacattc gggcggccga tgatggccac gggtatggcg tccttgtgct
      541 cttgaaggct ctcatcacgg tgccgaggaa gcagcgaaac aatgtggtcg aggagatcgc
      601 ctgtctcgag tccgtgcagt gcggagattc cgatgaccgg ttcgaatccc aacccataga
      661 aggcgtgcag cagatcttcc ctgttgggat tgtccacttt attgacagcg acaatcaccg
      721 gcctgcctgt tttgcgcaga taagcagcaa tggcatattc atctgccgta gggccatcca
      781 cagcgtcgcc cagaaataca accagatcgg attcttgtac cgcctgttgg atatttgcca
      841 acatggactg cgtgaggaca tcagcagcat cggacacgta gccaccggtg tcaatgagcg
      901 caaaaactcg gtttccccag gaaacatcac catagaccct gtcgcgagtg atcccgggtt
      961 ggtcgagtac aatggcctgc cggcgtccaa ccaagcggtt gaagagtgac gatttgccca
     1021 cattgggtct gccaactata gcgacaatgg gtttgcccat ggtgactcct cgagatgtcg
     1081 tgaccagcac gcctcagaac ggtagaagaa cacaaaaacg gaaatgactc aagagggagt
     1141 gcatccaaac actctgggag aactacgcct tgacagattc cgtaggaata ataaactaat
     1201 cattatcgcc aagagcaggc aagacttggg gatggccggc cggttactcc ggtgtgtttg
     1261 agtttatttc gaacgacgac tgttgttggg tcgtctaaca gccagacgtg tgagaaagga
     1321 tcctggtctt taggagcaaa tgaccaaagc gatatttgta aggcggggat ttcaccagtt
     1381 ccagccgtct cgacagcaac caaggccctg gaagcctatc gtggttctgg ctttcatcct
     1441 gctgcttgtc gtggggctga tgccggttcc gtcgtcgcgt ttggaaaaac gacttcagga
     1501 gtcctttcag gaagcctttg gtggggctgc gtctttttcc tcgtgcagta tccgaattcg
     1561 cgacttccgt ctctataccg acgagaccca acaacaactg cttctggaat gttcacgtgt
     1621 tcgtgtgggc tacaaccctg tgttctatcc gttgacaggt gcttacggtc tgtatctttc
     1681 caagccccgc ctgattgtac ccaacgcggt tgacgggcag tttcaaacgc ccaagtctct
     1741 ctcaaatgca tttaagggag attcagaagg aacatcaaat gttccctctt tcatccgcgt
     1801 ggacgatgcc cgcatcgaat atacatgcat ggtgacttcg gttcccgtat tgggagagga
     1861 cattgacata gccatcacgc ggctgagaag cgaaaagccc aaggcgctgg aatgtcgcgg
     1921 tcgagcactg tgggcgggac aaggcttgtc ctttacctcc gagggcacgg tgaatctgct
     1981 tgagaagtcc gccagccttt ccggcatcat ccgacccgcc acggggacgg agatctccgt
     2041 gaatcttctg ggatacaaga cagcgggaga ggtggtttct tctgccaccg agcaaggcgc
     2101 aggagaagac aaagattcag tgcctacagg tgctaaccgg acacaattca gcgccaagag
     2161 cgttccatgg acattgaacg cttcgatact ggaaagcctt ctgagcctgg atgtagcctt
     2221 gacaccgcag gaagggtttt tgacgctttc agaaacattg aagccctctt tgaagcaagg
     2281 aacgggaagg ttcaagtacg acagagctat ggggaagttt tccgttgagg atttcaccat
     2341 cagtctggac aactaccacg ccacggcgaa tggagaattc gggctgacgg gatacaagcc
     2401 gtttcgactc aaattttcca gccaggatat gccgtcggag gtcatccgaa cccttttggc
     2461 acaaaggcaa ccggagctgc cctttgtctt ggtgagtggc attctcggat tccagattga
     2521 gtcttccgga ctgatgacca atccagcggg gacattctct caaggaaggc ttgattgcca
     2581 gggatgcact ttcgagcatg ttgcaactgg ttttcgaatt accagcgtat ccggtgggat
     2641 tgacttctct cccgaagagg ctgtttttcg gcagttggag gcacgttaca cggacagaaa
     2701 gttttcgata gatggttcag tcaggggcga tccttacatc tgggatgatc ccgtggttga
     2761 tgtcaaagca cagggtgaaa tcgatctcat gcagacgggt aaattcatcc acgccctgat
     2821 tcctgcattc gtcggagaca ccaagatctc cggcaatgcc cttttggata ttgcagctac
     2881 agggcgtgtc agcgatccca acagtctgac ttttgacggg agcgtggatc tgcaaaatgt
     2941 aagtgtttcc ggtggtcttt tcccagttcc cttcaaccac gtttcagggc aagttgtttt
     3001 ccgggataac acaatgaccc tgaatgacct tcaagcaagc acgggaaaga cgaccgtagc
     3061 gttgcaggga acctacaagc gcggagagca gtcgtggcgg gaagggactg ttgacttgaa
     3121 acttgtcgga gttgccgaag cagaggaaat ctttcctgtt ctgcagcgca gcatgccaaa
     3181 gtatttggaa ggataccgag ttgcaggacc gttggagtac aatctctctg tcagtggcct
     3241 gctgtcgtca accgccgaga gaacctggtc aggaaatgct ctctt
//
LOCUS       DAOQUC010000289         3283 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_33506_length_3283_cov_4.534696, whole genome shotgun sequence.
ACCESSION   DAOQUC010000289 DAOQUC010000000
VERSION     DAOQUC010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3283)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3283)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3283
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33506_length_3283_cov_4.534696"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..377)
                     /locus_tag="PKH07_06235"
     CDS             complement(<1..377)
                     /locus_tag="PKH07_06235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34575.1"
                     /translation="MKSRKGFLKHRKVILAIGIITVVVLAVALMLKTKKKGSAEELLT
                     WKVERGDVIIDVVETGSLEAKDSKVIECEVEGQTRIITIVEEGTIISATDVINKRVLV
                     ELDSSDLRKKLDQEAITVQSASAS"
     gene            complement(427..2031)
                     /locus_tag="PKH07_06240"
     CDS             complement(427..2031)
                     /locus_tag="PKH07_06240"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNT34576.1"
                     /translation="MARFEKLILTLAVLLLFTGCGAEYYRRSADKEAYRIIQDKSQAV
                     EEMPAGFHIESATIPADATALFVPEELNLIDAVEIAVANSRAYQTRKENLYLQALSLS
                     SSRHEFDPIFSGKISGDLNHDAANRESFSGILSTGVSKMLATGADLSAAISTSAFRYI
                     SSGDAASAAGTAFSAGLVQPLLKGAGRDVTLENLTQAEREMVYGIREFVRYRKSFSIG
                     IAKRYYDLLQRADEIRNASRNYENLQYLRNRTENMAQAGRAASFEVDQAYQRELQAWD
                     NCVRTQERYSGALDEFKLDLGIPTDLPIRLATSELEIIETRGLEDFRITVEEAVQTAL
                     EKRLDIKTQWERVEDAKRKVTVAKNNLKPRLDLILDYSAKTDASQPLDFGDGEDSYSV
                     GADLDLPLDKKSDRNAYRRSLINLASAQRSLEQATDEVKLQVRDAYRALEQARQTYDI
                     QRESLRLAQQRAESTQLLQQAGRAVTRDVLEAEQDLLDAKNALTRALIAHLNARLDLF
                     IAMESLRIDEGGIWEEERQRSPLPSP"
     gene            complement(2116..>3283)
                     /locus_tag="PKH07_06245"
     CDS             complement(2116..>3283)
                     /locus_tag="PKH07_06245"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF002223.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HNT34577.1"
                     /translation="VFVGEERYILHLIPSGVLYPDTPCIIGNGVVVNPRVLLDERDEL
                     RAKGVAVDSDNLMISPNAHLIMPYHIALDRAADATQGIGTTGRGIGPAYVDKFGRKGI
                     TVGDLVLDPNVREKIIEATKERNKILSAVYGMEPLNPDEVADEYLEMVSEIKPMVGEV
                     NAQIRRAISAGKRILFEGAQGTMLDPDWGTYPYVTSSQTLASGAFVGAGMPAREMDQV
                     LGVAKAYTTRVGSGPFPTEMSDKDAEKIRNAGPVGEYGATTGRPRRCGWLDLPLLRRA
                     VAVNGVTSLALTRLDILSGRKEIPVCVAYEYQGKRWDFPPEWHGAWPEVKPVYEERKG
                     WQEDISEVRVFEDLPMAAQEYVRSIEETLEQSVGLISVGPARTQTIVRRQSFFR"
BASE COUNT          729 a          957 c          778 g          819 t
ORIGIN      
        1 gatgccgaag cactctgaac tgttattgcc tcctggtcga gtttctttcg aagatccgat
       61 gagtctaact ccacgaggac tctcttgttg atcacgtctg tggcactgat gatcgttcct
      121 tcttcgacga tcgtgatgat tctggtctga ccttccacct cacactcaat caccttggag
      181 tctttggctt cgaggcttcc cgtctccact acatcaatga tcacgtcgcc ccgttcaacc
      241 ttccatgtca gaagctcctc cgcagaccct ttcttcttcg tcttgagcat gagtgctaca
      301 gccaagacaa caacggtgat aatacctatc gccagaatca ctttccgatg cttcaagaaa
      361 cccttcctgc ttttcatctt tgtttctttg gtgtcctttc ccatctccga cgttggctga
      421 ctgttgtcac ggcgatggca ggggagatct ctgcctctcc tcttcccaga tacccccctc
      481 atcgatccga aggctctcca tggcgataaa caaatccaac cgcgcattca gatgagcgat
      541 caaggcgcga gtcagcgcgt tttttgcatc caacagatcc tgctccgctt ccagaacgtc
      601 gcgtgtgaca gcacgtccag cctgctgcaa cagttgtgta ctctccgcac gctgttgcgc
      661 cagtctcaaa ctttcccgtt ggatgtcgta ggtctgtcgg gcttgttcca gagcacgata
      721 cgcgtcacga acttgaagtt tgacttcgtc cgtggcctgt tcaagacttc gttgtgcgga
      781 ggccaggttg atcaaagatc ttcgataggc atttcgatct gatttcttgt ccaaggggag
      841 atcgagatcg gctcccacgc tataggaatc ctcaccatcg ccgaagtcca gaggctgaga
      901 ggcatctgtc ttggcggaat agtcaagaat caaatcgagg cgtggtttca gattattctt
      961 tgccacggtg acttttctct tggcgtcttc cactcgctcc cactgagtct tgatgtccag
     1021 tctcttctcc agtgcggttt gaacggcctc ttcaacggtg attcggaaat cttccagacc
     1081 tctggtctct atgatctcca actcgctggt cgccaagcgg atgggaagat cggtgggaat
     1141 tcccagatca agtttgaatt catccaaggc gccactgtaa cgctcctggg tgcgcacgca
     1201 gttgtcccac gcctgcaatt ccctttgata ggcttgatcc acctcaaagg aggctgcacg
     1261 gcctgcctgc gccatatttt ctgttcgatt gcgaagatac tgaagattct cataattgcg
     1321 tgacgcattg cggatctcat cggcccgctg cagaagatca taataccgct tggcaatacc
     1381 gatggagaaa gacttgcgat atctcacaaa ttcgcggatg ccgtatacca tctcccgttc
     1441 tgcctgggtg agattctcca gtgtgacatc tctgcccgcc cccttgagaa gaggctgaac
     1501 caaccctgcc gagaatgccg ttccagctgc ggaggctgca tccccgctgg agatgtaacg
     1561 aaaggcgctg gtgcttattg ccgcagacaa atctgcacct gtcgcaagca tcttggaaac
     1621 accagtggag agaattcctg agaaactttc ccggttggct gcatcatgat tcaggtctcc
     1681 ggaaatcttt ccggagaaaa taggatcaaa ttcatgtcgt gaggaactca gagaaagggc
     1741 ttgaagatag aggttttcct tacgagtctg atacgctctc gaattggcaa ccgctatctc
     1801 aacggcatct atcaggttca actcctctgg gacgaacaag gcagtggcat cggctggaat
     1861 cgttgccgat tcgatatgaa aacccgcggg catctcctca acagcctgac tcttgtcctg
     1921 tataatccga taggcttctt tatctgcact tcggcgatag tactcggcac cacaacccgt
     1981 gaacagcaaa agcaccgcca gagtcagaat cagcttttca aatcttgcca aagaactata
     2041 tcctctcact tgaagatacc catcatccga aaacgaccga ggacattccc tttctgggaa
     2101 acagagccgc cattcttacc tgaagaaact ctgtcttctc acgatggtct gtgttcgtgc
     2161 cggaccgacc gagatcagac cgacagattg ctccagtgtt tcttcgatag agcggacata
     2221 ttcctgggct gccatcggaa gatcttcgaa gacacgtact tcgctgatgt cttcctgcca
     2281 acccttgcgc tcttcgtaga caggcttgac ctctggccag gcaccgtgcc attcgggcgg
     2341 gaaatcccag cgcttgccct gatactcgta cgcaacgcag accggaatct ccttcctgcc
     2401 ggaaaggatg tccagtcggg tcagggcgag actcgtcacc ccattgacgg caaccgctct
     2461 tcgaagcaga ggcaggtcaa gccagccaca tcttctggga cgacccgttg tcgctccgta
     2521 ttcacccacc ggacccgcat tgcgaatctt ctcagcgtcc ttgtcggaca tctctgttgg
     2581 aaaaggaccg gagccaaccc gagtcgtata agccttggca actcccagaa cctgatccat
     2641 ttcacgggca ggcatgccgg caccaacaaa ggctcctgat gccaaagtct gagaggaagt
     2701 gacataagga taggttcccc agtcgggatc cagcattgtt ccctgcgccc cttcaaacaa
     2761 aatgcgcttt cccgcactga tggcgcgccg aatctgtgca ttgacctctc cgaccatggg
     2821 tttgatctcg gagaccatct ccagatactc atctgcaacc tcatccggat tcaaaggctc
     2881 cattccatag actgcggaca gtatcttgtt gcgctccttg gtggcttcga tgattttttc
     2941 ccggacgttg gggtcgagca ccaagtcccc aacggtgatc ccttttcttc caaacttgtc
     3001 aacgtacgcc gggccaatcc cgcgccctgt ggtgccaatt ccctgcgtcg catctgctgc
     3061 gcggtccaag gcgatatgat agggcatgat caaatgagcg ttggggctga tcatcagatt
     3121 atctgaatcc acagccactc cctttgcgcg aagttcatcc cgttcatcca acagcaccct
     3181 gggattgaca acaacgccat tgccgataat acacggagtg tccggataca ggactcctga
     3241 cgggatcaga tggagaatgt agcgttcttc tccaacaaaa acc
//
LOCUS       DAOQUC010000290         3275 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_33631_length_3275_cov_3.015528, whole genome shotgun sequence.
ACCESSION   DAOQUC010000290 DAOQUC010000000
VERSION     DAOQUC010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3275
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33631_length_3275_cov_3.015528"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1213
                     /locus_tag="PKH07_06250"
     CDS             <1..1213
                     /locus_tag="PKH07_06250"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNT34578.1"
                     /translation="AEAENAQKVARTVKSLNKETVVILGGHHASSLPESFLRETPDVD
                     YIVHGEGELTLAELVERIDGGQDVSDVQGIAYRRGDSVVVTPRRELIADLDALPLPAR
                     NKLDLNKYIPRSGTGNYLQLPTTGIMASRGCPYQCHFCSKGVWGTTIRFRSVESVIEE
                     IQVCMKDYGIRDFRFYDDALTLPKWELSRFCETLIRLNLGITWNCYSRVNHINEEKLR
                     LMKRAGCYHVKYGLEFGTEKALKLANKAATLEQARTAVALTKKVGLECKGNFMLGIPG
                     ETLEDCQTTISFAIELSPDLASFYPFDLIPGSEFYRRLKTGDPSVGSALPKALVQRMS
                     ERAYLRFYVRGGYIKQRLARLLMHPARESKVLGSGVYMMTRFFMTQILNQLVGVLVKR
                     NGKRELNQSAK"
     gene            1203..2474
                     /locus_tag="PKH07_06255"
     CDS             1203..2474
                     /locus_tag="PKH07_06255"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNT34579.1"
                     /translation="MRSDRSLNVLIMTPIFPLPLMSGGQVRLFNIIKRLSEYCRVSLL
                     SLVDSQQAHQIVHLSPYCTRLETVEATVFPSTFKRWLHALSPAEILRTAKRLVFLGRG
                     IPFQVCRFYHREMEQRLKRMLVEQQFDVIEGVYPQMAPYLLTARKVAPRAKFILEEID
                     LSFISKEREKPTKSGMNQWLASLEHDRMRRYSANLWPLFDRVIAMSETDRDSLLSLMP
                     TAKVSVVPNGVDTEFFVPPAKQGSPDRLKVAFLGGSQHYPNVDAMKFFATQIAPRIRC
                     SQSDFVFKIIGAFDGRLIPQLPFPTEFTGYVEDMRPHLCECSLLIVPLRIGGGTRLKI
                     LEAMSLGVPVLTTTIGCEGIEAVSGRDVIIVDDPQAFADQITAILADGRLRERLSRYG
                     RQLVEQRYSWPEIVESLVQVYQEVCCSGVVG"
     gene            2525..>3275
                     /locus_tag="PKH07_06260"
     CDS             2525..>3275
                     /locus_tag="PKH07_06260"
                     /inference="COORDINATES: protein motif:HMM:NF013959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP repeat protein"
                     /protein_id="HNT34580.1"
                     /translation="MKQTHMTTKTAINAVLAILALMQFESSYASVVVDARNADEDMAV
                     YGDRLLDGLGYPLLAADINGDGIKDVIVAATASDGLDQTEVNCGVVYVIFGSDSLPRS
                     AQMAADADITIYGVEHGINGAEGDEAGYALAAADFNHDGIDDLVIAAIYGDGENNSRP
                     DCGDVYIIYGRKDFPSLMDLEVDANVTIYGKDGGNGEGNPWDPDKGDLAGSALTVGDF
                     NGDGMADLAIGAVLGDEPNESQIHCGEVYILW"
BASE COUNT          752 a          787 c          946 g          790 t
ORIGIN      
        1 tgctgaggca gagaatgccc aaaaagttgc tcgaacggta aaatccctca acaaggagac
       61 tgtggtcatc ctcggtggtc accatgcttc ctcgctcccc gagtcatttc ttcgcgagac
      121 accggatgtt gattacattg tccacggaga gggggaactg actctggcgg aattggtcga
      181 aaggatcgac ggcggtcaag atgtatcaga tgtccagggg attgcatatc ggcgggggga
      241 ctccgttgtt gtcactccgc gcagagagct gattgctgat ctcgatgcac tgcctttacc
      301 tgcgaggaat aagttggatc tgaacaagta cattccccgc tcaggcaccg gcaactatct
      361 ccagttgccg acaacaggca tcatggccag ccggggttgt ccctaccagt gccatttctg
      421 cagtaagggc gtctggggaa caactattcg atttcgatca gtggagagtg tgatagagga
      481 aatccaagtt tgcatgaagg actatggcat ccgggatttt cgtttctatg acgatgcact
      541 cacgctcccc aagtgggagc tcagcagatt ctgcgagacg ctgatccgcc tgaatcttgg
      601 tatcacatgg aactgctata gtcgggtcaa ccatatcaat gaggaaaaac ttcgtttgat
      661 gaaacgcgct gggtgttatc atgttaagta tggtctcgaa ttcggtaccg agaaggcatt
      721 gaaactggcg aacaaagcgg ccactcttga gcaagccaga actgcggtag cgctcaccaa
      781 gaaggtaggt ctggagtgta aaggcaactt catgctggga attcccggtg agactcttga
      841 agactgccaa acgacgattt ccttcgcgat agagttgtcg cccgatctgg cctccttcta
      901 tccgtttgac ctaatcccgg gcagcgagtt ctatcggcgg ctcaagaccg gtgaccccag
      961 tgttggttcg gctctgccca aggcattagt gcagcgcatg agtgagagag cttaccttcg
     1021 cttttatgtg cgcggcgggt atatcaagca gcgcctggct cgactactca tgcatccggc
     1081 cagggagtcg aaggtactgg gcagtggagt ctatatgatg acccgcttct ttatgaccca
     1141 gatactcaat cagcttgttg gagtccttgt taagcggaat gggaaaagag agttgaatca
     1201 gagtgcgaag tgacagaagt ttgaacgttc tcatcatgac gcccatcttc ccactgcccc
     1261 ttatgtcggg tggacaggtg cgcctgttta atatcatcaa acgactgtcc gagtattgtc
     1321 gggtatcgct gctgtccctg gttgattcac agcaagccca ccagatcgtc catctctccc
     1381 cgtattgcac acgactggag acagtcgagg caacggtctt ccccagtaca ttcaagaggt
     1441 ggcttcacgc tctgtctcca gcggagatcc tccgcactgc caagagactg gtctttctgg
     1501 gcagagggat accttttcag gtgtgtcggt tctatcaccg agagatggaa caacggctca
     1561 agagaatgct tgtcgagcag cagtttgatg tcatcgaggg cgtctatcct caaatggcac
     1621 cgtatctcct gacggcgagg aaagtcgccc caagagcgaa gttcattctg gaagaaatcg
     1681 atctgtcatt catctccaag gaaagggaga agccgaccaa gtccggaatg aatcagtggt
     1741 tggcgtccct ggagcacgac agaatgcgtc ggtattcggc aaacctgtgg ccgctctttg
     1801 acagggtgat tgccatgtct gagacggaca gagactctct tctttcactt atgccgacgg
     1861 ccaaagtgtc tgtcgttccc aatggggtgg acacggagtt cttcgtacct ccagcgaaac
     1921 aaggctcgcc agatcggctg aaagtcgctt tccttggtgg ttcgcagcac tatccgaacg
     1981 tagatgccat gaagttcttt gcaacacaga tcgcgccaag gattcgttgc tcgcagagtg
     2041 atttcgtttt caagatcatt ggtgcgtttg acggtcgttt gatccctcaa ctgccattcc
     2101 caaccgagtt tactggatac gtagaggaca tgcgtcctca tctgtgcgag tgctcgcttc
     2161 tgatcgttcc tctgaggatt ggcggaggca caagactgaa gatccttgaa gcgatgtcac
     2221 tgggggtccc tgtgttgacg acaaccatcg gctgcgaggg tatcgaggca gtttccggga
     2281 gggatgtgat catcgttgat gatcctcaag cgtttgcgga tcagataacg gctatccttg
     2341 ccgatggtcg gttgcgagag aggctttcca gatacggacg gcaacttgtt gagcagagat
     2401 actcgtggcc tgagatcgtt gagagccttg tgcaggtcta tcaagaggtg tgttgttcag
     2461 gtgtggttgg atgagccggc gacattgtgt cgtttggcag atttgaaaac gaggactggt
     2521 ggacatgaaa caaactcata tgacaacaaa gacagcaatc aatgcagttc tggctatcct
     2581 tgcgctgatg cagtttgaga gttcctatgc cagtgtggtc gtagacgccc gcaacgcaga
     2641 tgaagacatg gccgtctatg gggatcgtct gcttgacggc ctgggctatc cccttcttgc
     2701 cgcggatatc aacggggatg ggatcaagga tgttattgtt gctgcgacag catctgacgg
     2761 cttggatcag actgaggtga actgtggagt tgtctatgtc atttttggca gtgattcttt
     2821 accccgctcg gcacagatgg cagccgatgc cgatatcacc atctatggag tcgagcatgg
     2881 catcaacggc gctgaaggcg atgaggcggg ttacgcactg gcagccgctg atttcaacca
     2941 tgatggcatt gacgatctgg tcatcgcagc gatctatggg gatggtgaaa acaacagccg
     3001 gcctgactgt ggggatgtct acatcattta cggtcgaaaa gactttccga gtctgatgga
     3061 tctcgaggtg gacgcgaacg tgaccatcta cggaaaggat gggggcaacg gagaggggaa
     3121 tccctgggat ccggacaaag gcgatcttgc tggaagtgcg ctgaccgtcg gggacttcaa
     3181 cggtgacggg atggcagatc tcgccattgg cgcagtcttg ggagatgagc cgaacgaatc
     3241 acaaattcac tgtggagagg tctatatact ctggg
//
LOCUS       DAOQUC010000291         3275 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_33633_length_3275_cov_2.825776, whole genome shotgun sequence.
ACCESSION   DAOQUC010000291 DAOQUC010000000
VERSION     DAOQUC010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3275
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33633_length_3275_cov_2.825776"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(78..1286)
                     /locus_tag="PKH07_06265"
     CDS             complement(78..1286)
                     /locus_tag="PKH07_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HNT34581.1"
                     /translation="MGNSFEAVKITDDVYWVGAIDWAIRDFHGYHTGRGSTYNAYLIL
                     ADKVTLMDTVKSPFFEEMMGRIRSVIDPAKIDYLISNHSEMDHSGSIRQTIATIQPEQ
                     VFASPMGAKALVEHFHLSQEITPVKDGETLSLGNRTLTFLETRMLHWPDSMVSYLAEE
                     QLLFSQDGFGMHLATSERFADEIPSDILEFEGAKYYANILLPYSALVLKLAKRIEELG
                     LRFSIIAPDHGPIWRKNPEKIIGLYVRWAEQKPGNRAVVAFDSMWGSTELMARSVADG
                     LAGEGTKTKVLNLRASHRSDLPTEVLGAGALLVGSPTLNNGIFPTLADNLCYLKGLKP
                     QNLIGGAFGSYGWSGESVKQLEALLKEMGVDVACDGLRIKYVPDQKALAQSREWGAAV
                     ARQLKGRLGI"
     gene            complement(1312..1806)
                     /locus_tag="PKH07_06270"
     CDS             complement(1312..1806)
                     /locus_tag="PKH07_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015947122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HNT34582.1"
                     /translation="MAIQFNADEIFEIAEQIERNGAKFYRRAAKGFDSSETLQLLGDL
                     AIMEDQHEQTFSMMRKQLGSGDRSEMTFDPYGEAALYLKAMADGYVFNVREDPSNRLT
                     GEETLQNILTTAIGLEKDSIVFYLGIKELVPAGLGRDKIDGIIKQEMGHVTILTKHLA
                     ALEQ"
     gene            complement(1833..3104)
                     /locus_tag="PKH07_06275"
     CDS             complement(1833..3104)
                     /locus_tag="PKH07_06275"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNT34583.1"
                     /translation="MKPESMNLSLFDLVRCFASAMDLVSSVVGEHQRRVSCIAYRLAS
                     EMGLSEKDQEDVSLAGAMHDCGVFSLQEKLALMSADSDLPNIHRHAEIGYRALKKFPA
                     FERFALIIRYHHLPWHNGAGAEKNGERVPIESHIIHLADRIDTFINRKEYIIGQTDDI
                     LRRVQERSDDFAPQALAAFDSLQHREFFWLDVVHGAPSPVFDPSMRSSDRSLGLSDLE
                     VISRVFCQLMDFRSHFTATHSSGVAATAEALARLCGFSDVECQVMRVAGQLHDLGKFA
                     IPVELLEKPAHLTDEEFRLIRSHTYHTNRILKTIPELNVVNAWGSLHHEKLNGRGYPF
                     HMDDLELSEGSRIMAVADVFTALTEDRPYRKGMTRQGTLDIMKRMAHDGALDQNILET
                     LERHFEFLESIRIKAQEAEAMAYYDVSQVQN"
BASE COUNT          805 a          947 c          781 g          742 t
ORIGIN      
        1 ctgcatgtcg ttcccgtctt catccggcag cagtcaaaca gaatgcccgc acaacgctca
       61 gactaccccc gaacagatta gatccccaaa cgtcccttca actggcgcgc caccgcagct
      121 ccccattccc gactctgagc caaagccttc tggtcgggta catacttgat gcgtagtcca
      181 tcgcaagcca cgtcaacgcc catttctttc agaagcgctt cgagctgttt gacagactca
      241 ccgctccaac cataggaacc aaaagcccca ccgataaggt tctggggctt gagtcccttc
      301 aggtagcaga ggttgtccgc caaggttggg aatatgccat tgttgagcgt tggagagcca
      361 accaacagag caccggctcc aaggacttct gtcggaagat cacttcgatg agaggcacgg
      421 agattcagaa cttttgtctt tgttccttcg ccagcaagtc catcggcaac agaacgagcc
      481 atcaactcgg tgctccccca catcgagtca aatgcaacaa ccgccctatt tccaggcttc
      541 tgctctgccc atctcacgta gagaccgatt atcttctccg gattcttccg ccagatgggt
      601 ccatggtcgg gagcaatgat ggaaaaacga agaccgagtt cctcgatccg cttcgccaat
      661 ttcaaaacca atgccgaata gggaagaaga atgttggcgt agtactttgc cccttcaaac
      721 tcaaggatat ctgaaggaat ctcatccgcg aatcgttcac tggtggcgag atgcatccca
      781 aacccgtcct gcgagaaaag cagctgctcc tcggccagat acgaaaccat gctgtcaggc
      841 caatggagca ttcgggtctc aagaaatgtc aacgtccggt tgccaagact gagagtctca
      901 ccatccttca ccggagtgat ctcttgactg agatgaaaat gctcgacgag ggcttttgcc
      961 cccatcggag aggcgaagac ttgctccggc tgtatggtgg caatggtctg tcggatgctg
     1021 ccggaatgat ccatctcgga atgattggag atcagatagt ctattttcgc cgggtcaatg
     1081 acagagcgga ttcgacccat catctcctca aagaaaggcg acttgacggt gtccatcaga
     1141 gtcaccttgt cggcaaggat cagataggca ttgtatgtcg agcctcttcc cgtgtgatag
     1201 ccgtgaaaat cgcgaatcgc ccagtctata gcaccaaccc aatagacatc gtcagtgatc
     1261 ttgactgcct caaacgaatt ccccatcgaa cacactcctt caactcggaa attattgctc
     1321 cagtgcggcg agatgcttcg tcaggattgt gacgtgaccc atctcctgct tgatgattcc
     1381 atcaatcttg tctcgcccca agccagcagg aacgagttcc ttgatcccca ggtagaacac
     1441 gatggaatcc ttctccaagc caatagcggt cgtaagaatg ttctggagcg tctcctctcc
     1501 cgttagcctg ttcgagggat cttcccgaac gttaaagacg tatccatccg ccatggcctt
     1561 cagatagaga gccgcctcac cgtagggatc aaaggtcatc tcagatctgt caccgctgcc
     1621 aagctgtttg cgcatcatcg aaaaagtctg ctcgtgctgg tcttccatga tcgccagatc
     1681 tcccagcagt tgcagggtct ccgagctgtc gaagcccttc gccgctcgac ggtagaactt
     1741 ggcaccgttg cgctcgatct gttccgcaat ctcaaatatc tcatctgcgt tgaactgaat
     1801 cgccatagcc cgaaatcctc ccaaaatcaa ctctagttct gtacttgtga aacatcataa
     1861 taagccatcg cttccgcctc ctgagctttg atgcgaatgg actccaaaaa ctcaaagtgt
     1921 ctttccaaag tctccaagat gttctgatcc aaggctccat catgtgccat gcgcttcatg
     1981 atatcaagcg tgccttggcg ggtcattccc ttgcgatatg gacgatcttc agtaagggca
     2041 gtaaacacgt ctgccacagc cataatccga gaaccttctg acagctccag atcatccata
     2101 tggaatggat agccgcgacc gttgagtttc tcatggtgca gcgatcccca ggcattcaca
     2161 acattcagtt caggtattgt cttcaggatt cgattcgtat gataggtgtg ggaacggatc
     2221 agtcgaaact cctcgtccgt cagatgcgcc ggtttctcca gcagttccac aggaatggca
     2281 aatttcccca gatcgtgcag ttgtccggca acacgcatca cctgacattc cacatcagaa
     2341 aagccgcaca gcctcgccag tgcctccgcc gtcgcagcaa cacccgagga gtgtgtcgct
     2401 gtaaaatgac tccgaaaatc catcagttga cagaacaccc tcgagatcac ctccaaatca
     2461 gagagcccca gcgagcggtc tgaagaacgc atggacggat caaaaacagg actcggagcg
     2521 ccatgaacaa catccagcca aaagaactcg cgatgttgca gcgagtcgaa ggccgcaagc
     2581 gcttggggtg caaaatcatc cgaacgctct tggacgcgtc ggagaatgtc atccgtctgt
     2641 cctataatgt attccttccg attgataaac gtatcaattc ggtctgcaag atggataatg
     2701 tggctttcga tgggaacgcg ctcgccattt ttctccgcgc cagctccgtt gtgccaaggc
     2761 aggtgatgat acctgatgat cagagcaaac cgctcaaacg ctggaaactt cttcaatgca
     2821 cggtaaccga tctcggcgtg tcggtgaatg ttcgggagat cactgtcggc gctcatcaag
     2881 gcaagtttct cctgcagtga gaacactccg cagtcgtgca tagcgccggc aagggaaaca
     2941 tcctcctgat ctttctcgga gagacccatc tcagaggcca gcctgtacgc gatgcaggag
     3001 actcggcgct ggtgctctcc caccaccgag ctgaccaaat ccatggcgga agcaaaacac
     3061 ctcaccaaat caaaaagtga caagttcatg gattccggtt tcactgacat actccacaga
     3121 ttctcagtcg tcttcgctcc taagaaacct gactccagag gagttggctt cctctctgaa
     3181 caagcgcatc gcaagggtgg gcatagccca cgtgcgtgca ggcgcaggcc tgctctttgg
     3241 gaacctgact ccggaggagt tggtctcctc tctga
//
LOCUS       DAOQUC010000292         3274 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_33646_length_3274_cov_3.512582, whole genome shotgun sequence.
ACCESSION   DAOQUC010000292 DAOQUC010000000
VERSION     DAOQUC010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3274
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33646_length_3274_cov_3.512582"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1142
                     /locus_tag="PKH07_06280"
     CDS             <1..1142
                     /locus_tag="PKH07_06280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="phenylacetate--CoA ligase family protein"
                     /protein_id="HNT34584.1"
                     /translation="NEVHLERFSQVPFLTKQIIREQGRALISEDCPRRGSYPNTSGGS
                     TGEPVRFLQDARYKAHNWADVLLYNRVLGKELGMREVKLWGSERDILQGGTGWITQMG
                     NFLYNRLLLNSFLMTPDLMREYVSRINHFKPYSLWTYVESAFELARFVDREKLSLVPI
                     PVTITTAGTLTESVREFIEKHLQTKVHNQYGSREVGPIACECPMRNGLHLFEWTILLE
                     TVDEKGQSTEPGKEGEIVVTLLSNYSMPLIRYRIGDRGTLTERSCPCGRQTRLLETVS
                     GRIVDHFRLSDETLVGGEYFTHLLYHRPWVQKFQFVQDRFDHVSVYLVVEQQPQPSEL
                     DEIRSKVQVVMGKQCEVTFQFVEEVPPTTSGKRRYTVCAIAKEQS"
     gene            1139..2107
                     /locus_tag="PKH07_06285"
     CDS             1139..2107
                     /locus_tag="PKH07_06285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HNT34585.1"
                     /translation="MNSGTNKSRMRYVFQTSQFVLAVILIVFLLWKLDIGEVLKAFHT
                     LSVGWLLLSWLCILSVFYCRALVLCWIAENKARLAPTRVFLLNILGSFFTNFLPSRAG
                     GDLVKGVYLAEYFPSMSDAYAAMILQRLVGIVGAFAVIVIATLVLFPFKQALWVLVPA
                     VVIVAGISVLAKFSELWETHRKSSWAMRMERFVITRLMLRLVDAMVIYRHEKRRFVAP
                     FLFSMAITCQGILGAFLAGRAVGSSATFTQIFVASTAAQISAFIPLTPGGLGVGESSF
                     VLACLSFGVPKEEAFATAVLVRLILVSISLLGGAFYLFNPIRRNEP"
     gene            2104..3048
                     /locus_tag="PKH07_06290"
     CDS             2104..3048
                     /locus_tag="PKH07_06290"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNT34586.1"
                     /translation="MSREGLLRRYRPFYLLARLARRVLPYCLSQKLLSRRSVPAAQLY
                     CGKGNCSWTYLRIIQQTDLSLLFERPESLVVLETGTGFDNPLSAPLLLTGIRQLLLLE
                     PYVGARADHHRLQERLRYLVDCAESDPEFPLSRRQTVSDLFGQNSKPHQMPANVQILD
                     RFWEDTGLPDGSVDLILSASVLEHLRKPDAVLRESSRILRPDGWMLHLVDLRDHFFRY
                     PFEMLKYSPRAWSLLTTHTGGSGFQNRWRLPQWLDALEQCGFNTQVIPQLTLRDEMLL
                     ERPFFHSDFQGFSDGDLQIAVAILVSRRGSTVNLPTQS"
     gene            complement(3035..>3274)
                     /locus_tag="PKH07_06295"
     CDS             complement(3035..>3274)
                     /locus_tag="PKH07_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNT34587.1"
                     /translation="NDDDFIFDNQILVQAHYFGYRIGEVSCPTRYFSEASSINFRRSL
                     KYGLGVLGVSIQFVLARIGVVRPRIFRKDDQFRIG"
BASE COUNT          718 a          847 c          853 g          856 t
ORIGIN      
        1 acaacgaagt acatctcgaa cgattctctc aggttccatt cctcaccaag cagatcattc
       61 gcgaacaagg ccgtgctctc atcagtgagg attgtccccg tcgcggcagt taccccaata
      121 catccggtgg ctcgacggga gagcccgtcc ggttccttca ggatgcacgc tacaaagcac
      181 acaactgggc tgatgttctc ctctacaacc gagtcttggg aaaagagctg ggaatgcggg
      241 aagtcaaact ctggggatcg gaacgtgata ttctccaagg cggaaccggt tggatcacac
      301 agatgggaaa cttcctctac aatcggctgc ttctgaacag ctttttgatg acgcccgatc
      361 tgatgcgcga gtatgtgtca agaatcaacc atttcaaacc gtactcacta tggacctatg
      421 tggaatcagc gtttgaactt gctcgatttg ttgaccgtga gaaactgtcc ctcgtgccta
      481 tcccggtcac gatcaccaca gcgggcacct tgacggaatc ggttcgtgag tttattgaaa
      541 agcaccttca aaccaaggtt cataatcaat atggatctag agaggtcggc ccgatcgcct
      601 gcgagtgtcc tatgcgcaac gggcttcatc tgttcgagtg gacgattctt ctggaaactg
      661 ttgacgagaa aggccagagc accgaacctg gcaaggaggg agagatcgtc gtcacactgt
      721 tgagcaacta ctcgatgcct ctgattcgat accgcattgg agacagaggg actcttactg
      781 agcgatcctg tccctgtggg agacagactc gactgctgga aacagtgtcc ggtcggatcg
      841 tggatcactt ccggctgtcc gacgaaacgc ttgtgggcgg ggagtatttc acgcatctgc
      901 tgtatcatcg cccgtgggtt cagaagtttc agttcgttca agaccgattc gatcatgttt
      961 ctgtctatct cgttgtggaa caacagccgc agccctctga actggatgag atccgatcca
     1021 aggtccaagt cgtcatgggt aagcaatgcg aggtaacctt tcagttcgtt gaagaagtgc
     1081 caccaacgac ctcaggaaag aggcgctaca cggtctgcgc catcgcgaaa gagcaatcat
     1141 gaactcaggc acaaacaagt cacggatgag atatgtcttt caaacttccc agtttgtgct
     1201 ggcagtgatc ttgatcgtct ttcttctctg gaaactggac attggggaag tcctgaaggc
     1261 atttcacact ctgagtgttg gatggttgct gctgtcatgg ctttgcattc tcagcgtctt
     1321 ctactgtcga gcgctggtgt tgtgttggat tgcagaaaac aaagcacgac tcgcaccgac
     1381 acgggtattt ctgttgaaca ttcttggcag tttctttacc aatttcctgc cgtcacgcgc
     1441 cgggggcgat ttggtcaagg gcgtctatct tgcagagtat tttccttcga tgagcgatgc
     1501 ctatgcagcc atgatcctgc agaggcttgt cggtattgtt ggagcttttg ccgttatcgt
     1561 gatcgccacg ctggtgctct ttcccttcaa gcaggcgctg tgggtgcttg tgcctgctgt
     1621 tgttatcgtg gcaggcatat ctgtcctggc gaagttcagt gaattgtggg agacacacag
     1681 aaagagttct tgggctatga ggatggagcg gttcgtcatc actcggctca tgctgcgact
     1741 tgttgatgcg atggtcatct accgacatga gaagaggcgg tttgttgcac catttctgtt
     1801 ctccatggcc atcacctgtc agggaatcct tggtgctttt ctggctgggc gtgcagtggg
     1861 cagttcggcg acattcaccc agatattcgt cgcctcgacg gccgcccaga taagcgcatt
     1921 catcccgctc acaccggggg ggcttggtgt tggagagagc tcttttgttc tggcatgtct
     1981 ttcctttggc gtcccaaagg aagaggcctt tgccacagca gttctggttc ggctcattct
     2041 tgtctccatc agccttctcg gaggagcgtt ctacttgttt aaccccatca ggagaaacga
     2101 accatgagtc gggaaggact cctgcggcgc tatcgtcctt tttacctcct tgcacgactg
     2161 gctcgaaggg tgctccctta ctgtctttca caaaagctct tgtctcggcg ctcagtacct
     2221 gccgcccagt tgtactgtgg gaagggaaac tgctcgtgga cgtatttgag aatcatccag
     2281 cagaccgatt tgtctctttt gttcgaacgg ccggagagtc ttgtcgtcct ggagacaggc
     2341 actggctttg acaacccact gtcagcaccg ttgttgctga caggaatcag acagttacta
     2401 ctgcttgaac cctatgtcgg tgccagagcc gatcatcatc gtcttcagga acgtcttcga
     2461 tatctcgtgg attgcgccga gtcagatccc gaatttccgc tctccaggcg acagactgta
     2521 tctgatttgt tcggtcaaaa ctcgaagccc caccaaatgc ccgccaacgt gcagattctt
     2581 gaccgattct gggaagacac gggtttgcct gatggttctg ttgatctgat tctttctgcc
     2641 tcagtgctgg aacacctcag aaagcctgat gcagtcttga gggaatcgtc aagaatcctc
     2701 agaccagatg gctggatgct gcatcttgtt gaccttcgtg accacttctt tcggtatcct
     2761 tttgagatgc tgaagtactc cccccgggca tggtctctcc tgacaaccca cacgggaggc
     2821 tcaggattcc aaaatcgctg gcgactccct cagtggctcg atgcgctcga acaatgcggg
     2881 ttcaacaccc aggttattcc tcagttgact cttcgggatg agatgttgct agaacggcct
     2941 tttttccact ccgactttca gggattctct gatggggatc tgcaaatcgc cgtagctatt
     3001 ctggtttcga gaagaggatc tactgtaaac ctgcctaccc aatcctgaat tggtcatcct
     3061 tccgaaagat tctcgggcga accacaccta tcctggcgag cacaaactgg atggagacac
     3121 caagaactcc caatccatac ttcaaactgc ggcgaaagtt gattgatgag gcttcggaaa
     3181 aataccttgt tgggcaggac acttcgccga ttcggtatcc aaaatagtgt gcctgaacga
     3241 gaatctgatt atcgaagatg aagtcgtcat catt
//
LOCUS       DAOQUC010000293         3272 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_33675_length_3272_cov_3.854523, whole genome shotgun sequence.
ACCESSION   DAOQUC010000293 DAOQUC010000000
VERSION     DAOQUC010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3272
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33675_length_3272_cov_3.854523"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..393
                     /locus_tag="PKH07_06300"
     CDS             <1..393
                     /locus_tag="PKH07_06300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34588.1"
                     /translation="MGNSGGGTTTYFAGCLEPRISVSMPSCYVCTFRGCIGSIDHCAC
                     NYVPNIMQYFEMGDLACLIAPRPLIVVAGLQDNIFPIAEVRKNFDLIQSIYAKAGVAD
                     KCRLVVGESGHQFYPQLGWPAFRELAQW"
     gene            483..1532
                     /locus_tag="PKH07_06305"
     CDS             483..1532
                     /locus_tag="PKH07_06305"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNT34589.1"
                     /translation="MNSRERVLTSLAWQEPDRVPIQIYLTPEIAGKLADRFKGRDLLE
                     VFGVDFRHVGAAWRGTVKPPQGEVYYDIWGVGYKNQRYEFGTYPEAYDLSLARIETMD
                     DFVRYPWPSVDDFDVSKVWERCDAIKDYSVCCGGAGHPDIINGVSRGRGMQRVIEDIM
                     LEDEVGIAIIDRRVDFCYRELERILEAGRGKIDIVCLGEDCGTQNGRLFSQKVFDDFF
                     RPRLKRFYDLAHRYGAKAMMHSCGDTHEIMPTFIEMGLDVLDAMQPEPPGMQPEAIRK
                     ACFGKLAFCGLISTQQTLPRGTVEECRVEARHRLDVIAKGGGYIFAPAHCIQPDTPVE
                     NILAVFEQALGRPLE"
     gene            1597..2145
                     /locus_tag="PKH07_06310"
     CDS             1597..2145
                     /locus_tag="PKH07_06310"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HNT34590.1"
                     /translation="MKILAIVGSRNAEGMTATATKSLLKGAQAAGAEVETVFLPWKNI
                     ERCRQCDDSGWGICRTTGNCAIEDDFAHLVNAIRSSDRIVLATPVYFSDLSESMKAFL
                     DRLRRIGMHEKGKDGIEAKVAVAICVAGGGGGGAPQCACIMDRILRTCGFDLVETIPV
                     RRQNLDLKKSLLETTGGWLASS"
     gene            complement(2251..>3272)
                     /locus_tag="PKH07_06315"
     CDS             complement(2251..>3272)
                     /locus_tag="PKH07_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNT34591.1"
                     /translation="RIAEEPEVMWLTDNTMIDAWLGDDAAWLTDAQVLAAPCRDDDDT
                     LAAVMLFRDAAEPFEDSHYELLEAICPVFGSHLVKVINIDQSPIGRTPRSNPATYVKL
                     FDPIRTLFAQLPEAKIRGFGPGHFSFNVPGGRCEACEGDGHKRIEMHFLADVFVTCDV
                     CRGKRFNRETLQVKFKNHSISDVLDLTVEQARALFAHQMRVQRILRTLVDVGLGYIRL
                     GQSAPTLSGGEAQRIKLAKELCRASTGRTLYILDEPTTGLHFADIEKLLKVLNRLADK
                     GNTIIIIEHNLDVVKSADWIIDLGPDGGDAGGEVVAQGPPEKLVRSPASYTGQFLNQV
                     LNGSR"
BASE COUNT          714 a          848 c          982 g          728 t
ORIGIN      
        1 atgggcaatt ccggaggcgg cacgacaacc tactttgccg gatgtctgga gccgagaatc
       61 tcggtttcga tgccctcttg ttacgtgtgc acgttccgtg gctgcatcgg ttcgatagac
      121 cactgtgcct gcaactacgt gcctaacatc atgcagtact tcgaaatggg agatttggct
      181 tgtctgattg cgccaagacc attgatcgtc gtagcgggac tacaagacaa catcttccca
      241 atcgcggaag tccgaaagaa cttcgacttg atccagagca tttatgccaa ggcgggcgtt
      301 gctgacaaat gtcgtctggt ggttggtgag tcagggcatc agttctatcc gcagctgggc
      361 tggcctgcgt ttcgagagct ggcgcagtgg tagatgaagt ggaaactctg tcaatgtgac
      421 aggagcattg cgccccgact gggagataat gcaactccaa ggacggagag aggagaattc
      481 atgtgaactc gcgtgagcgt gtccttactt cattggcatg gcaggagccc gacagggttc
      541 ccattcagat ctatctcact ccggagattg ccgggaaact ggcagatcgc ttcaaaggtc
      601 gcgatcttct ggaagtcttt ggcgtggatt tccggcacgt cggagcggcc tggcgaggga
      661 cggtcaagcc accccaggga gaggtctact acgacatttg gggggttggc tacaagaacc
      721 agcgctatga atttggaacc tatcccgaag cctacgattt gtcgcttgcc cgaattgaaa
      781 cgatggacga ctttgtccga tatccctggc cgagcgttga tgatttcgat gtctccaaag
      841 tctgggaacg ttgtgatgca atcaaggact attctgtctg ctgtggggga gccggtcatc
      901 ccgacattat caacggtgtt tcgcgaggac gcggcatgca gcgcgtgatc gaggacatta
      961 tgctggaaga cgaggtcgga atcgcaatca tcgatcggcg cgtggacttc tgttaccggg
     1021 aactcgaacg gattcttgag gcagggcgtg gcaaaataga tattgtctgt ttgggcgagg
     1081 actgtggaac gcagaacggc cgcctgttct cgcagaaggt gtttgacgat ttcttccgtc
     1141 cccgtctcaa acgattctat gatctcgcgc atcggtacgg cgcgaaggcg atgatgcact
     1201 cgtgtggaga cacacacgaa atcatgccca cgttcattga gatgggtctc gatgttcttg
     1261 acgccatgca acccgagcca ccaggaatgc agccagaggc aatacgcaag gcatgctttg
     1321 gcaaacttgc gttctgcggc ttgatcagca ctcagcagac actgccgaga ggaacggttg
     1381 aagagtgtcg cgtcgaggcg cgacaccggc tcgatgtcat cgccaaaggc ggcgggtata
     1441 tctttgcgcc agcgcattgc atccaacccg acacgcccgt tgagaacatt ctcgccgtgt
     1501 tcgaacaggc attgggacga ccgctcgaat aggtgtttta cgccgacgca aggatgctct
     1561 gcatacaagt atctttgtaa gaccgggagg catatcatga agatcttggc aattgttgga
     1621 agtcgaaatg cggaagggat gactgcgaca gccacaaagt ccctgctgaa gggagcgcaa
     1681 gccgccggag ccgaggtgga gacggtattt ctcccttgga agaacatcga acgttgtcgg
     1741 caatgcgacg actcaggatg gggaatctgc cgcacgacgg gcaactgtgc tatcgaggac
     1801 gatttcgccc atctggtcaa cgccattcgg agttcggaca ggatcgttct ggcaactcct
     1861 gtttacttca gcgatctcag cgagagcatg aaagccttcc tggatcgact gaggagaatc
     1921 ggcatgcatg aaaagggcaa ggacggcatt gaggccaagg ttgccgttgc catctgtgtc
     1981 gcggggggcg ggggtggcgg tgctccccag tgcgcctgca tcatggaccg cattctgcgg
     2041 acctgtggat ttgatcttgt cgagacgatc ccagtccgac gccagaacct tgatctgaag
     2101 aagtcacttc tggagacaac cggcggttgg ttggcctcat cataataccg cttgccaacc
     2161 ggttcagatt tcccgctcga ggggagtcct gatcgcgtgc ttgactgaaa ctgtcagagc
     2221 agagcgatgt ggcaatcacc cagcggggat tcagcgcgaa ccattcagca cctggttgag
     2281 gaattgcccg gtataggagg caggagatcg aaccagtttc tctggtggcc cctgtgccac
     2341 gacttctccg ccagcgtccc caccgtcggg tcccaaatca atgatccagt ccgcagattt
     2401 cacaacatcc agattgtgtt cgatgatgat gatcgtgttg cccttgtctg caaggcggtt
     2461 gaggactttg agcagttttt cgatgtcggc aaaatgaaga ccggttgtgg gttcgtccag
     2521 gatgtagagt gtcctgcctg tggaagcgcg gcacagttcc ttggcaagtt tgatccgctg
     2581 tgcttcgccg ccagagaggg taggcgccga ctgtcccaac cgtatatatc cgagacccac
     2641 atccacaagt gttctcagga ttcgctgaac cctcatctga tgggcaaaca gagcccgagc
     2701 ctgttccacc gtcagatcca gcacgtcgga gatgctgtga ttcttgaact tgacctggag
     2761 tgtttctcgg ttgaagcgct ttccacgaca cacatcacaa gtcacaaaca catcagcaag
     2821 gaagtgcatt tcaatgcgct tgtggccgtc gccttcacag gcttcacatc ggcctcccgg
     2881 cacattgaat gaaaaatggc ctgggccgaa cccccgaatt tttgcctcgg gaagttgtgc
     2941 aaacagtgtt cgaatgggat cgaagagttt cacataagtg gctgggttgg agcggggcgt
     3001 gcggccgatg ggactctggt caatgttgat gaccttgacc aggtgcgagc cgaacacggg
     3061 gcagatggct tcaagcagtt cgtagtgact gtcctcgaac ggttcggcgg cgtcgcggaa
     3121 gagcatgacc gcggcgaggg tgtcgtcgtc gtcgcggcag ggggcggcca gcacctgggc
     3181 gtcggtcagc caggcggcat catcgcccag ccacgcatcg atcatcgtgt tgtcggtcag
     3241 ccacatcacc tccggttcct cggcgatgcg ag
//
LOCUS       DAOQUC010000294         3268 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_33734_length_3268_cov_3.929972, whole genome shotgun sequence.
ACCESSION   DAOQUC010000294 DAOQUC010000000
VERSION     DAOQUC010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3268)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3268)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3268
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33734_length_3268_cov_3.929972"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1520
                     /locus_tag="PKH07_06320"
     CDS             <1..1520
                     /locus_tag="PKH07_06320"
                     /inference="COORDINATES: protein motif:HMM:NF012509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="galactokinase family protein"
                     /protein_id="HNT34592.1"
                     /translation="IYGDSGALIYRRQKGHINALLQFAYAFGEERECLLVRCPSRINL
                     LGMHVEHRGGYVNPIAIGSELVVVASSRRDDRFHIRNVESDLFPDTSFALHELLPNDP
                     QMDWVTFVNHVQIERGHWGNYVRAALLRLQMEFPDKPFCGMDMMVYGDIPRGAGVSSS
                     SALVVATALAAVNLNRLEVDRKHLTVLCGEGEWYVGTRGGAGDQGAMLLGKQNCITHM
                     QFFPLHYETIPLPAGYVVVLCRSFVDAHKAAGARSVFNERVATYDIGLMLIRKRYPQL
                     ASRIRYLRDVSPGHLGVDDAWIYRLLQNVPESMNRDELLAILPEETERLEGLFSTHED
                     PPTGYQIRQVLLYGIAECAKADYAGSLLRRGDIEEFGRLMSISHDGDRVVRYDSKGFA
                     VSHNSEISDKDLDRLIEQCCSDRCPESAKIYRQSGGYACSHPRVDQIVDILCQIPEVL
                     GARVTGAGLGGCVVALVPSAYAQEAINALDSQYYGVHGLPEGAEVCVSVEGACTL"
     gene            complement(1554..2504)
                     /locus_tag="PKH07_06325"
     CDS             complement(1554..2504)
                     /locus_tag="PKH07_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013164783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 and DUF1722 domain-containing protein"
                     /protein_id="HNT34593.1"
                     /translation="MNEKIRIGISSCLLGENVRFDGGHKLDRFLRDTLGQYVEYVGVC
                     PEVECGFTVPREPMRLVGDPVAPKLLTVRTGQDHTERMKTWALRRVKELEKEELCGYI
                     FKSGSPSSGMERVKIYDANGVPSKVGVGLFAQAFMDHFPLLPVEEEGRLHDSPLRENF
                     IERIFVLKRWRDVLASGRKLSSLVDFHTRHKLLIMSHSNKHLKDMGALVAKAKGLPKK
                     GVFQEYFELLMGSLKLRATVNKHTNVLMHVMGYFKRALTADEKKELLEVIENYHQQYV
                     PLIVPITLLNHYVRKYEQPYLKEQYYLNPHPVELQLRNHV"
BASE COUNT          762 a          823 c          877 g          806 t
ORIGIN      
        1 gcatatatgg ggattccgga gcgctcatct atcgaagaca aaagggacat atcaacgctc
       61 tgctgcagtt tgcgtatgct tttggagagg agcgggaatg tctcctcgtg cggtgtccgt
      121 cgcgtatcaa tcttcttggg atgcacgtag agcatcgcgg aggctatgtg aaccccatcg
      181 ccatcggttc ggagctggtt gtggtcgcgt cctcaaggcg agatgaccga tttcatatac
      241 ggaatgttga gtcagatctc tttccagaca cttcgttcgc tcttcacgag cttctgccca
      301 acgatccgca gatggattgg gtcacgtttg tcaatcacgt tcagatagaa agagggcact
      361 ggggaaatta cgtacgagcc gcgctgttga ggctgcaaat ggagttcccc gacaagccgt
      421 tctgcggcat ggacatgatg gtgtatgggg acatcccgcg aggggctgga gttagttcct
      481 cctccgcttt ggtggtagcc acagcgctcg ccgcagtgaa tctgaacaga ctggaagtag
      541 atcgaaagca tctcactgtt ctttgcggcg aaggggagtg gtatgtcggt acaaggggtg
      601 gtgctggaga tcagggtgcc atgcttctgg gcaaacagaa ctgcatcacg cacatgcagt
      661 tttttcccct ccattacgag accattccgc tgccggccgg ttatgttgta gtgctgtgtc
      721 gctcgtttgt ggacgcgcat aaggctgctg gagcgagaag cgtgttcaat gaacgagtgg
      781 caacctatga tatcggactg atgttgatcc gaaagagata tccgcaactg gcttcacgaa
      841 ttcgatacct tcgggatgtg agtcctggac atcttggagt ggacgacgcc tggatatatc
      901 ggcttttgca gaatgttccc gagtccatga atcgggatga actgctggcg attctgcccg
      961 aggaaacaga acggctggag ggtttgtttt ccacccacga agatccgccg actggttacc
     1021 agatccggca agtgcttctc tatggaatcg ccgaatgtgc gaaggcagac tacgcgggat
     1081 ctcttctgcg tcgaggagac atcgaggaat tcggcaggct catgagcatt tcccatgatg
     1141 gcgatcgggt ggtgcggtat gacagcaagg gttttgccgt ctcacacaac tcggagatca
     1201 gcgacaagga cctcgatcgt ttgatcgagc aatgctgctc ggacagatgt cccgaatccg
     1261 cgaagatcta ccgtcaatcg ggaggctatg cctgcagcca tcccagggtg gatcaaatcg
     1321 tggatatcct ctgtcagatt cccgaagtgc tcggagcacg ggtgacaggc gcgggtttgg
     1381 gcggttgcgt tgtggcgctc gtgccgagtg cgtacgctca ggaagcgatc aatgccttgg
     1441 actctcagta ctacggcgtc catggtcttc ctgaaggtgc cgaagtctgt gtttcggtgg
     1501 aaggtgcatg caccctgtaa actgctcgga tcgagacgag agcatccgga ttttcaaacg
     1561 tggttgcgca gttgcagttc gaccggatgc gggttcagat aatactgctc cttgagatag
     1621 ggttgctcat actttcgcac gtagtggttg agcagtgtga tcggaacaat cagaggcacg
     1681 tattgctgat gatagttctc gatgacctca agaagttcct tcttctcgtc cgctgttaga
     1741 gcgcgtttga agtaccccat tacatgcatc aggacattgg tgtgcttgtt gactgtggcg
     1801 cgcagtttga gactgcccat gagtagttca aaatactcct gaaaaactcc cttcttcggc
     1861 agacccttag cttttgcgac caaagctccc atatccttca gatgcttgtt gctgtgggac
     1921 atgatcagca acttgtggcg cgtgtgaaaa tcaaccagag aggagagttt tctgcctgac
     1981 gccagaacat cgcgccaccg cttaagcacg aagattcgct caatgaaatt ctctcgaagc
     2041 ggcgagtcat ggagccgtcc ctcttcctca accggcagca gaggaaaatg atccataaac
     2101 gcttgagcaa aaagcccgac cccgaccttg gacggtacac cgtttgcatc atagattttc
     2161 acccgctcca tgccgctgct cggagaaccg ctcttgaaaa tgtacccgca cagttcttct
     2221 ttctcaagtt ccttaacacg ccgcagtgcc caggttttca tgcgttccgt atggtcttga
     2281 ccagttcgaa ctgtgagcaa ctttggagcg actggatcgc caacaagcct catcggctct
     2341 ctggggactg taaacccgca ttccacctcg ggacaaacac ccacatactc gacatattgc
     2401 cccagtgtat cgcgaaggaa gcgatccagt ttgtggccac cgtcaaagcg cacattctcc
     2461 ccaagaagac aagaactgat accgattcgg attttctcgt tcatctcttt cctctctttg
     2521 ccaatcgccc gatccaaaag atcaagggct tctcaagatg atcaaactgc cgaacgtggt
     2581 cctcagtgtc aactctgctc tttcgtccgg cctcgtctct atgtcggatt atgccgaata
     2641 cccgtcgttc cggcaatgcc cgatcacaaa tgtcaagaca gcacaaggga atcgggttcc
     2701 catacaccaa gttgacgcgt gctgtcagga aggcttttcc ttgagtcaaa gatcgcactg
     2761 tcccgaccga agaaatatct ggtttcagat ttccagtaaa cctttttgcg ccgtgcggcg
     2821 tctctttgtt tgaagtgagt tgaatacatc cgtggtgaac gaagtacaga ggatcggtgc
     2881 tgttatcgct tcgtacatca gcaaacagag aagcgatgtg agctcttcgc gattttgact
     2941 tcattgtgta cggaggaatt tgagatagtc tggatcaaac cgaagcaagt ctccaaggac
     3001 accgtcgtcc tcagaaatgc catcctgcag ttccggattg ccagatgatg agccaagaaa
     3061 gaccgtcgtg ttcgcaagtc ggcacagaaa cggatatcaa cagtacatta tccctcaaaa
     3121 aaatacttga cagtatttcg gagagtgtat actgttctag tagtgtatac agttaagtga
     3181 ggattccaat gattatcaga atagagaaag cggccggtgt tccgatcacg agacagattc
     3241 tcgaccagat tcgaacacac tgtgcgtc
//
LOCUS       DAOQUC010000295         3262 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_33830_length_3262_cov_3.305582, whole genome shotgun sequence.
ACCESSION   DAOQUC010000295 DAOQUC010000000
VERSION     DAOQUC010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3262
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33830_length_3262_cov_3.305582"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..502
                     /locus_tag="PKH07_06330"
     CDS             <1..502
                     /locus_tag="PKH07_06330"
                     /inference="COORDINATES: protein motif:HMM:NF024820.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNT34594.1"
                     /translation="PRRAGQGDGAALLDGGQAAKAIQECRRIAGLLEKLEPEVAIKRY
                     KMILELTSDDLHAHQGLIRVYRRQNQKEQAFEHLVAAGRLLLQQRHHHKAAQAFWQAI
                     QIEPRDEEVYRHLALCQENLGEPHHAWNTLESRADVLAKSGRVHEAVEEYLHLADLYL
                     QEGAAD"
     gene            517..>3262
                     /locus_tag="PKH07_06335"
     CDS             517..>3262
                     /locus_tag="PKH07_06335"
                     /inference="COORDINATES: protein motif:HMM:NF024820.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNT34595.1"
                     /translation="MRAFQTQSSANYQKAALVLNKALALDPDNLIIHERLASVYERQG
                     NTRSAAERMQFIAHTAKRAGNGALLEKTLLKLVDLLPDDPEVLQQMAHFRTKQGQLRE
                     AVVVLENLLGIHERQGALPKIASTCEQILALEPQRSDIREKLIGIQEEKGSQKEVLAE
                     RRALAESLVEQGNLQDALQQFRLILSVEPEDIAVLERMAELSDRTGLKEQSHDGYVKL
                     ASLAAQPEQALQIYQKAVQTFATKPRTWQKLAECAEATGQKDVAVAAFEKASQLFLDT
                     SEPVLAEKCLRKVRELAPDSQRAREMLIRLLKSQGKRGEALEELLDLAGILREKGETT
                     EACRLYQEAHSENPDSLAAAFQLGEVYQETGQTQAFCQLYEKLGAQRSAEGDLEGSLD
                     LYKRLLGVDPDNVDALQGLATTLRAMGDTKQAVLNVRRLAEILCAQAQDSSAEAVRPL
                     LLSRARQCLWDAIEWASEDADLHCQMAAVEALRGDKEQAARILVDFCKRSLLKFRSEE
                     TQTGEVAAKETFEKLLAQTENIVGDSPLVHEMRSEVNSAFGQIQEAAQDLLNAGDLHQ
                     KRGNPAAALLDFERARNVLPDSDQPLERIFALCKQRGDANAAVAAAWQLAKRFRDRSQ
                     GEALDQMLGEIAVLTPSDARVHEWLVLSALESGQQQKAIEKALLGADALIAQQHFQEA
                     VVLCRTAREKIGLNPTLSEKIMECLVQRQDIRGAIAEATDLATQYQQEGVLDRAIALY
                     QRAITWEPRNTVLRKSLAEQLASAGRIDDAKREWLELAGLYTQSGLAEKAVEVLRGVR
                     NRFAQDFSIRHQLVQLLLKEGMNRSAAQEGSALLDEIWGQIQTSEGDQKRGLLQESRQ
                     LAEQVLAANPDLGDIVAVYAEILFCLGDESAYADQKIRQATLLRETNDYTGATNCLQE
                     S"
BASE COUNT          758 a          810 c          986 g          708 t
ORIGIN      
        1 cccgcgacgg gctgggcaag gtgacggtgc tgcacttctg gacggtgggc aagctgccaa
       61 ggcgattcag gaatgtcggc ggattgccgg tcttctggag aaactggagc cggaagttgc
      121 catcaagcgc tacaagatga tcctcgaact gacttccgat gatctgcatg cgcaccaagg
      181 cttgattcgt gtctatcggc gtcagaatca gaaggaacaa gcctttgagc acttggttgc
      241 ggcagggagg ctcctgcttc agcagcgtca ccatcacaag gcggctcagg ctttttggca
      301 ggcgatccag atcgagcctc gcgatgaaga ggtctatcgt catctggctt tgtgtcagga
      361 gaatcttggc gagccacacc acgcgtggaa tacgcttgag agccgggcgg atgtcctggc
      421 gaaatcaggc agagtgcatg aagccgtcga ggagtattta caccttgctg atctctatct
      481 gcaggaaggc gctgcagact gaacaggtta gaagcgatga gggcatttca gacacagtct
      541 tctgccaact accagaaagc cgctttggta ctcaacaagg cgttggcgct ggatccagat
      601 aatctgatta tccatgagcg gttggcgtct gtctacgagc gacaagggaa tacgcgctct
      661 gcagccgagc ggatgcaatt catcgcccat acggccaaaa gagcaggcaa cggtgccttg
      721 ctcgaaaaga cccttttgaa actggtggat ctgcttcctg acgatcccga agttctccaa
      781 cagatggcgc atttccgaac caagcagggg caactcaggg aagcagtagt ggtcttggag
      841 aatctgcttg gcatccatga acgacaggga gcgttgccca aaattgcctc aacctgtgag
      901 cagattctcg ctcttgaacc tcagcgtagc gacattcgtg agaaactcat cggtattcag
      961 gaagagaagg gatctcagaa agaggtactt gccgagcgac gtgctcttgc cgaaagcctc
     1021 gttgagcagg gcaatcttca ggacgctctt caacagtttc gcctcattct ttctgttgag
     1081 ccggaagata tcgcagttct ggaacggatg gcggaactca gcgatcgaac gggtctcaaa
     1141 gagcaatctc atgacggata tgtgaaactg gcttcgcttg cagctcagcc cgaacaggct
     1201 ctccagatct accagaaggc tgttcagacg tttgcgacca aacctcgtac ctggcagaaa
     1261 cttgctgaat gtgccgaagc gacggggcag aaggacgtag cggtcgccgc ttttgaaaag
     1321 gcttcgcagc tgtttctgga caccagtgag cccgtgcttg ctgagaagtg tttgcgcaaa
     1381 gtcagggaat tggcgcccga ttcacagcgt gcgcgtgaga tgttgattcg tttgctgaag
     1441 tctcaaggca aacgtggtga agcccttgag gaactcctgg acctggctgg aatccttcgc
     1501 gagaaggggg aaacgactga ggcgtgtcgg ctctatcaag aagcgcattc ggagaatccc
     1561 gattcgctgg ctgctgcttt tcaacttggg gaagtgtatc aggaaacagg tcagacacag
     1621 gctttctgtc aactctatga gaaactgggg gcacaacgaa gtgccgaggg tgacctggag
     1681 ggatctttag atctctacaa acggctgttg ggagtggatc ccgacaatgt tgatgcgctg
     1741 caggggcttg ctacgacact gcgggcaatg ggtgacacga aacaggcggt tctcaatgtc
     1801 cgtcgcctgg cagaaattct gtgtgctcaa gcccaggaca gctccgcgga ggcggtgagg
     1861 cctctgcttc tttctcgggc acgtcagtgt ttgtgggacg cgatagaatg ggcttctgag
     1921 gatgcagatc tccactgtca gatggctgca gttgaagcgt tgcgaggtga caaggaacaa
     1981 gcagcgcgaa ttcttgtgga cttctgcaag aggagtcttc tgaagtttcg gtcagaggag
     2041 acgcagacgg gtgaagttgc ggccaaagag actttcgaga aactgcttgc ccagacagaa
     2101 aacatcgtcg gtgattcgcc acttgtgcat gagatgcgct cggaggtcaa cagcgccttt
     2161 gggcagatcc aggaggctgc gcaggatctt ctgaatgctg gggatcttca tcagaagcgg
     2221 ggcaatccag ccgctgcatt gctggatttt gaacgtgcgc gcaatgtgct tcctgactct
     2281 gaccaaccat tggagcgaat ctttgcactg tgtaagcaac ggggtgatgc aaatgctgcg
     2341 gttgctgcag cctggcaact ggcaaagcga ttccgtgacc gatcccaggg ggaagccctc
     2401 gatcagatgc tcggagagat cgcagttctc actccttccg atgccagagt tcatgagtgg
     2461 cttgtgctga gcgccctgga aagcggtcag cagcaaaaag ccattgagaa agccctgctc
     2521 ggagcggatg ccctgattgc acaacagcac tttcaggaag cggttgtcct ttgtcgtacg
     2581 gcgcgggaga agatcggtct gaatccaacg ctctccgaga agatcatgga gtgtctcgtc
     2641 caacgccagg acattcgagg cgcgatcgcc gaagcaacgg atctggccac acagtaccaa
     2701 caggaagggg ttcttgatag agccattgcc ctctatcagc gtgccattac ttgggagcct
     2761 cgaaatacgg ttctccgcaa atcgcttgct gagcaactgg cctcggcggg caggattgat
     2821 gatgccaagc gcgagtggct cgaactggcc ggtctctata cacagtccgg tctggcggag
     2881 aaggcagtcg aagttctgag aggcgtgaga aaccgttttg cgcaggactt ttcgatccgt
     2941 catcaacttg tgcagttgct tctgaaagaa gggatgaacc gatcagccgc tcaggaagga
     3001 tcggctctgc tggatgagat ctgggggcag attcagacgt ctgagggcga tcaaaagaga
     3061 ggccttcttc aagagtcgcg tcagttggct gagcaggtgc tcgcggccaa ccccgatctt
     3121 ggggatattg ttgccgtcta cgctgagatt ctcttctgtc tgggggatga atcagcctat
     3181 gcggatcaga aaatccgtca ggcgactctg ctcagagaga ccaacgatta cactggtgca
     3241 accaactgct tgcaggagtc gc
//
LOCUS       DAOQUC010000296         3259 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_33888_length_3259_cov_3.316792, whole genome shotgun sequence.
ACCESSION   DAOQUC010000296 DAOQUC010000000
VERSION     DAOQUC010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3259
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33888_length_3259_cov_3.316792"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..513
                     /locus_tag="PKH07_06340"
     CDS             <1..513
                     /locus_tag="PKH07_06340"
                     /inference="COORDINATES: protein motif:HMM:NF027666.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4976 domain-containing protein"
                     /protein_id="HNT34596.1"
                     /translation="PLIFAGPGIEEGNRSEQGLVSLLDLLPTLCEYAGIRTQPRREGI
                     SLVPWLAGDTSSDPHHFVACQWHTEWGFTIEPGRMIRTPRFKYMRYLEEDGEQLYDLQ
                     TDPGEVHSLVNDPSYEQVLDEHRTLLKQYIAATGDPFFSLGWKADTRWRSHPPGYRNH
                     RGDAAPTADA"
     gene            565..1803
                     /locus_tag="PKH07_06345"
     CDS             565..1803
                     /locus_tag="PKH07_06345"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HNT34597.1"
                     /translation="MNMTLSIILMSLLCFLLTCAECLASLSAQGVTLQEDEKTVQIVS
                     VNRWDLLFEYVAEAWPGLRIGGSPKAEYSVSAARKDLDDLARLNLKVLRVSATLYWST
                     PMKKTFYEDSKRWWSAFEQMLDDCDQRGIRLVLLLNHHSQLFPDLGHESRQDFFRNRD
                     SVSRQLHDNYIREVVSRFRNRKTLYFWEIANELNLGENLAFHDPEGLKHRDHLVGMET
                     LMNYPIVRSASNHYTTQDTVNYMRETAELIRTLDQKHLIGSGHSTPRPAAMHLWRAQQ
                     THGRGDWTQDSLEENIEYIRLIHPDPVDLISIHYYEDAQKLFGGELGNLENIATLMAI
                     SREIGKPLYLGEIGTPSREYASEESLAWLSKALEMIRKEAVPLTLFWTWNDPGRDLFL
                     NVGQTDKAIEIISNTAAQPD"
     gene            1886..>3259
                     /locus_tag="PKH07_06350"
     CDS             1886..>3259
                     /locus_tag="PKH07_06350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34598.1"
                     /translation="MKRFHLILVSVFLALCSVGEARDRDINLWPLLTLDSDDLSTDLQ
                     YLIPLGRIAENESERVNRLFPFHLYKRHLATDFTSLDLLWPLFNVTNSPGEFRKLTLL
                     PFQGTWEKDGEYLATVLPLYWQFRDDRFEGVVILPAWWVTGVMDTDQYRMGVAPLFWS
                     WNTSSKGFWFAPVLSVRDKGSSFLGILPVWARWKEETPKSEGFWFMPVYWHRSAGDRY
                     CLSVVPIYWQWQNGRVVFPIYWENDSGRVVFPFYWQADDHQVLFPLYWSLDYGKLFFI
                     FPFYGRFRGVNESWTAIMLPAYLESTRGDYKQKDILWPLIHWGSDEKTRRELSFFPFY
                     SQTWKENYEQGNFTYSADETRILLFQWGRNGYSYKEKNGGTVDQSSEGSSLFPFLFLR
                     KTENRNVTASGSEQWVRDNTSTTVFPFFWKQRNTDWEAQIENRPEEIREGHSLNLFPF
                     FWSGEKHV"
BASE COUNT          758 a          814 c          883 g          804 t
ORIGIN      
        1 cctctcattt tcgcggggcc tggaattgag gaaggcaatc gttcggaaca aggtctggtt
       61 tcgctgttgg atttgttgcc cacgctgtgc gagtacgcgg gaatccgaac tcagccgcgg
      121 agggagggga tatccctggt gccgtggttg gcgggagata cctcaagcga tccacatcac
      181 tttgttgcgt gtcaatggca caccgaatgg ggttttacta tcgagccggg tcgcatgatt
      241 cgcactcccc ggtttaaata catgcgctat ctcgaagagg acggagagca actgtacgat
      301 ctgcagaccg atcctggaga ggtacatagt ctggtgaatg atccctcgta tgagcaggtt
      361 ctcgacgagc accgcacatt gttgaaacag tacatcgccg caacggggga tccctttttc
      421 agcctcggat ggaaagcgga cactcgctgg agaagccatc caccggggta caggaatcat
      481 cgaggcgacg cagcacctac agccgatgcg taaacagtct tagacgcaag ccgtgagcca
      541 tccaggaact ccaggagtgt gaaaatgaat atgaccttat ccatcatctt aatgagtctc
      601 ttgtgttttc tgctgacgtg tgctgagtgc cttgcctcgc tttcggctca gggcgtgact
      661 cttcaggaag atgagaagac agtgcagatc gtctcagtga accgatggga tctcctgttc
      721 gaatatgttg ccgaagcctg gcctggtctt cgcatcgggg gttcacccaa ggcagagtac
      781 agcgtctcgg cagcgcgaaa ggatctggat gacctggctc gactgaatct gaaagtcctt
      841 cgcgtttcgg caacactgta ttggtcaaca cccatgaaga agactttcta cgaagactcc
      901 aaacgctggt ggagcgcctt tgagcagatg ctcgatgact gcgaccagcg tggcattcgc
      961 cttgtgctgc ttctcaatca tcactcgcag ttgtttcccg acctcggtca cgagtccaga
     1021 caggattttt tccgaaatcg agatagcgtc tcacgccaac ttcatgacaa ctacattcgg
     1081 gaagtggtca gccgttttcg aaaccgcaag accctctact tctgggaaat cgccaacgag
     1141 ttgaatctgg gagagaatct tgcctttcac gatccggaag ggctgaagca tcgcgatcac
     1201 cttgtcggca tggaaaccct tatgaactac cccatcgtgc gtagcgcaag caatcactac
     1261 acaacacaag atacggtcaa ctatatgcgt gagacagccg agttgattcg aaccctcgat
     1321 cagaaacatc tcatcggttc aggccattcg acacctcgtc ccgcagcgat gcacctctgg
     1381 cgagcacagc agactcatgg aagaggtgac tggacgcaag actcccttga ggaaaacata
     1441 gaatacatcc ggctgattca ccccgatccg gttgacctga tctcgatcca ctactatgaa
     1501 gacgcgcaga aactcttcgg cggcgaactg ggcaatttgg aaaacatcgc cacactgatg
     1561 gcgatcagcc gtgagatcgg caaaccgctt tacctgggtg agattggaac tccaagtcga
     1621 gagtatgctt ccgaggaaag cctggcctgg ctttcaaaag cactcgagat gattcgaaaa
     1681 gaagctgttc cgctcaccct tttctggaca tggaatgatc cgggacgtga tttgtttctg
     1741 aacgtcggtc agaccgacaa ggccattgag attatctcga atacagcggc gcagccggat
     1801 tgagtggaca gattcaggat gatcctcgta tcatcaatag ggttcccctg tagggaagat
     1861 tctatgaggc gaagaggtgg cggcgatgaa acgatttcat ttgattcttg tttctgtgtt
     1921 tctggctctc tgttccgtgg gtgaagcgcg tgaccgagat atcaatcttt ggcctttgct
     1981 gactctggac tccgacgatc tttcgacaga ccttcagtat ctgattcctc tgggaagaat
     2041 cgctgagaat gagtctgaaa gggtcaacag gctctttccg tttcatcttt acaaacgcca
     2101 tcttgcgacg gatttcacgt ctctggattt gctatggcct ctgttcaatg tcaccaacag
     2161 ccctggcgag tttcgcaagc tgactctgtt gccttttcag ggcacttggg agaaggatgg
     2221 tgaatacctc gcaacggtac tgccgcttta ttggcagttc cgagatgacc gttttgaagg
     2281 tgttgtcatc cttccggcgt ggtgggtcac aggggtcatg gatacggatc agtaccgcat
     2341 gggtgtggca ccgcttttct ggtcttggaa cacctcgtca aagggcttct ggtttgcacc
     2401 ggtattgagc gttcgggaca aggggagcag ttttcttggc atcctccccg tctgggcacg
     2461 atggaaagag gagaccccca agtcagaagg attctggttc atgccggtct attggcaccg
     2521 gtctgcgggt gacaggtact gcctttcggt ggttcccatt tactggcaat ggcaaaatgg
     2581 aagagtcgtc tttccgatct actgggagaa tgattcgggg cgggttgtct ttccgtttta
     2641 ctggcaggct gatgaccatc aggtgctttt tccattgtat tggtctctcg actacggaaa
     2701 actctttttc atatttcctt tctacggtcg ctttcgtggc gtgaatgaga gttggactgc
     2761 catcatgttg cccgcctatc ttgaaagcac gagaggcgac tacaagcaaa aggacattct
     2821 ctggcctctg attcactggg gcagtgatga gaagacacgc agggagttaa gcttttttcc
     2881 cttctactcc caaacgtgga aggagaacta cgagcaagga aacttcacct attcagccga
     2941 cgaaacgcgg attctccttt ttcagtgggg acgcaatggc tattcataca aggagaaaaa
     3001 cggcgggact gttgaccaga gttcagaagg ttcttctttg tttccctttc tgtttctgcg
     3061 gaagaccgag aacagaaatg tcaccgcgtc gggcagtgag caatgggtga gagacaacac
     3121 gagtacaacg gtgttcccct tcttctggaa gcagcgcaac acagactggg aagcccagat
     3181 tgaaaaccgt cctgaggaga ttcgagaagg tcattcgctg aatctctttc cattcttctg
     3241 gagcggagag aagcacgtc
//
LOCUS       DAOQUC010000297         3244 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_34145_length_3244_cov_2.468172, whole genome shotgun sequence.
ACCESSION   DAOQUC010000297 DAOQUC010000000
VERSION     DAOQUC010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3244
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34145_length_3244_cov_2.468172"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..761)
                     /locus_tag="PKH07_06355"
     CDS             complement(<1..761)
                     /locus_tag="PKH07_06355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34599.1"
                     /translation="MSTPSTRAAQNSEVPTPQSLGEILGQLGLADTPENRQIVAALIT
                     QGLPLDQQHVQLLSSLQGNPELAAILLGLGIELSDDILAQILDLGQATTASGQVLKEV
                     LELLQQVLVTRILDSDSTKMVESLIAQIRQTVMIAETGATDLQIKDFLALLVSATAGA
                     TAESVSGAVQTSLDTFLLSLSDNLNDISVLLGREMLSGTVRDTLLKRLSESRQHISST
                     AMGQILMRTFHQAASSRESSLVFFPLLWHEEPGTLT"
     gene            complement(863..1621)
                     /gene="tpiA"
                     /locus_tag="PKH07_06360"
     CDS             complement(863..1621)
                     /gene="tpiA"
                     /locus_tag="PKH07_06360"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012189471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HNT34600.1"
                     /translation="MRRKIIAGNWKMYCGTPQEAADLAGQLAKQVGSLTDRDVILCPP
                     FTALAATVQAVKGTNIAVGAQNMYPESKGAFTGEIAPEFLLSLGCTYVIIGHSERRTY
                     FKETNEFINKKVKCALAAGLNPIMCLGETLEERQANKINDVVKDHVQGGLAGMSAAEA
                     AKVVLAYEPVWAIGTGLTAEPKDAEAVHQFIRQVLTDMFDKATAEVIRIQYGGSVKPD
                     NAKTLLTMPNIDGALVGGASLKADSFTGIIKYEG"
     gene            complement(1749..2813)
                     /locus_tag="PKH07_06365"
     CDS             complement(1749..2813)
                     /locus_tag="PKH07_06365"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF002528.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HNT34601.1"
                     /translation="MEPVNVLVLSGGPSTERMVSLESGRMVSEHLDSERYHVVPAQIS
                     RQGLWYLAGELESDTHNMGTQEEAEALLPFQKRPLLEVGRSIDRILLGEEIDVAFIAM
                     HGSYGEDGTIQGLLESFGVRYTGSGVCASALAMDKVRSRHMFMQHHLSVPRWMTFDRN
                     QWAGKSQDCLEAIRSRLGLPCVVKPSNQGSSIGVHLVQDAASLEQAIVDTLSLARRVV
                     IEEYVQGTEITCGVLEVSAQAKALPIVEIVSQRNFFDYEAKYDPRLNEEIVPARIGEA
                     VADSASKAALKAHRALGCAGFSRTDMIVVGETPIVLETNTIPGLTPVSLFPKAAAAAN
                     IEFSRLLDIMVEGALSDQSY"
BASE COUNT          747 a          922 c          832 g          743 t
ORIGIN      
        1 gtcagtgttc caggctcttc atgccagaga agtgggaaaa acaccagcga tgactctcta
       61 gaggaagcgg cttggtggaa cgttcgcatg agaatctgtc ccatcgcggt gctggagatg
      121 tgctgacggc tttccgaaag gcgcttgagc agtgtatccc ggactgttcc agaaagcatc
      181 tctcgtccga gaagaacgga gatatcattc aggttgtcac tcaacgagag gagaaaagtg
      241 tcgagagaag tctgcacggc accggacacg ctttcagcgg ttgcccccgc ggttgccgaa
      301 acaagcagcg ccaggaaatc cttgatctgc agatccgttg cacccgtctc cgcgatcatc
      361 acggtctgcc gaatctgcgc gatcagggat tcaaccatct ttgtagaatc tgaatcaaga
      421 atccgcgtca caagaacttg ctgaagcagt tccagaacct ccttgagaac ctgtcccgat
      481 gctgtggtgg cttgcccaag atcgagaatc tgagccaaga tatcatctga cagctctatc
      541 ccaaggccga gcaaaatcgc cgccagttct ggattgccct gcagcgagct gagcagttgc
      601 acatgctgtt gatccagggg caaaccctga gtgatcaacg ccgcgacgat ctgtctattc
      661 tccggcgtat ctgccaaccc cagctgaccc aggatctctc cgagactctg gggcgtcggc
      721 acttcagaat tctgtgctgc tcttgtggag ggtgtagaca aagagggttc cctgacttgg
      781 gaaccctctt gaactgtcgt tggcttcccg agctgaggac tgactggttg cgtcgggatt
      841 gggtcaacca tgagttgtga actcagcctt cgtacttgat gatccccgta aaggaatcgg
      901 ccttcagact ggcaccacca accaatgcac cgtcaatgtt tggcatggtc agaagggtct
      961 tcgcgttgtc gggcttcacg ctgccaccgt actggatgcg gatgacttcg gcggtggcct
     1021 tgtcgaacat gtccgtcaaa acttgtcgga tgaactgatg gacggcttcg gcatccttcg
     1081 gctcggcggt caagccggtt ccaatcgccc aaacgggttc ataggcgaga accacttttg
     1141 ccgcctcagc ggcggacatg ccagcgagac caccctgaac gtgatctttg acgacgtcgt
     1201 tgatcttgtt cgcctgtcgc tcttcgagcg tttcaccgag acacataatc ggattcagtc
     1261 ctgctgccaa agcgcacttg accttcttgt tgatgaactc attggtctct ttgaaataag
     1321 tgcgccgttc tgaatggccg atgatgacgt acgtgcagcc caagctcaga aggaactcag
     1381 gagcaatctc gccggtgaac gctccctttg actcagggta catgttctgt gcaccgacag
     1441 caatgttggt gcctttgacc gcttgaactg tggctgccaa tgcggtgaac ggtggacaca
     1501 gaatgacgtc ccgatccgtc aacgacccga cctgttttgc cagttgtcca gccaggtcag
     1561 ccgcctcttg tggcgtgcca cagtacatct tccaatttcc cgcaatgatc tttcttcgca
     1621 tctcttctct ttcctccata gggattttat ctaaagcgga ttcatcatct gggaaatata
     1681 ccaactgttc ccatacggag cgtctctctc cgtctggacg atgtccgcct ttgatttctg
     1741 tccagttact aatacgactg atcggacaag gcaccctcaa ccataatgtc caacagtcgt
     1801 gaaaactcga tgttggccgc cgcagcggcc ttcggaaaca ggctcactgg agtcaatcca
     1861 ggtatcgtat tggtctccag aacgattggt gtttctccga ccacgatcat gtccgttcgt
     1921 gaaaaccccg cgcatcccag cgctcggtgc gccttcagag cagccttaga ggcactgtct
     1981 gcaaccgcct caccaatgcg cgccggcacg atctcctcgt tgagcctcgg atcgtacttg
     2041 gcttcgtagt caaagaagtt cctctgcgag acaatctcga caatgggaag cgccttggct
     2101 tgtgctgaaa cctcaagcac gccgcacgtg atctccgttc cctgaacgta ttcttcaatg
     2161 acgactcttc gggcaagcga cagcgtgtct acgatcgcct gctccaacga tgcggcatcc
     2221 tgcaccagat gaacgccaat gctggagccc tgattgctcg gtttgaccac acagggcaac
     2281 ccgagtcgcg aacgaatcgc ctcaagacaa tcctgacttt tccccgccca ctgatttcgg
     2341 tcgaaagtca tccaacgcgg cacggacaga tggtgctgca tgaacatgtg acgggagcgg
     2401 accttgtcca tcgccagagc gctggcacac actccagagc cggtatatcg aactccaaaa
     2461 gactccagaa gaccttggat ggtgccatct tcgccgtaag agccgtgcat ggcaataaat
     2521 gcaacgtcta tctcttcacc cagaaggata cggtcaatac tccgtcccac ctcgagcaag
     2581 gggcgtttct gaaaaggaag caaggcttcc gcctcttcct gggtgcccat attgtgagtg
     2641 tcagattcga gctcaccggc caaataccac aatccctggc gtgatatctg cgccggtacg
     2701 acgtgatacc gctcggaatc gaggtgttcg ctgaccattc tccccgattc cagagaaacc
     2761 attctttcgg tcgagggacc gccagaaagg acaagaacat taacgggttc caaggttctg
     2821 cctcaattga gatgaaaaaa gacattcgga tggagccgaa cacccgagtc ctgagcattt
     2881 ctgcatgaga gtcttgctca aaaaagggaa ggcagattct agcccccgcc gttgccgtgt
     2941 gtcggacagg tttaaaagcc agccctcaac aggtgggcgc ctaaaatggt cattaagacc
     3001 acggaaaggt tccctattct acttagtggc tcaaaacaag tagaacatca cgagcaacag
     3061 caggtggtag aatctgcctt ccctgtcgct ctctattgtt aatctaccaa gaatcccccg
     3121 agtgcgcaag cccttttgga gcaaagaggc atatcacatc caaaggtgtc acgctccttc
     3181 gggcaacacg ccggaatatg tatcagatcg gatgtgtgtc agtcctgcct tgtcatagag
     3241 gcag
//
LOCUS       DAOQUC010000298         3237 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_34255_length_3237_cov_3.424261, whole genome shotgun sequence.
ACCESSION   DAOQUC010000298 DAOQUC010000000
VERSION     DAOQUC010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3237
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34255_length_3237_cov_3.424261"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1333
                     /locus_tag="PKH07_06370"
     CDS             <1..1333
                     /locus_tag="PKH07_06370"
                     /inference="COORDINATES: protein motif:HMM:NF019325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HNT34602.1"
                     /translation="EELLTAESGGVIGLFASTGRGCPPHHEIYLRALHECLFVRGINS
                     IGALSAMGKILFQTQDKATDPIFMFQIFGDPLTRLRLPEVKGHLAVNPSQIQSRYGGR
                     VLVSEQPGLSLQGKQYAFIANARGELIPEPKMLSHGSADLPVGFDVPADLAPGTCSAT
                     IFSASMSPTQPATVLLGQFECLPREPLHEEALPSEARPNLRIETADISFSDFSPVNGQ
                     TVFITVRVHNTGHRSVRDVEVRAYDGDPQTGGLVLNDDVQWPFRTIALIRPGESETIR
                     LRWDPFQNAGEHRIWVQLDPSNLIEEENKQDNLASRPIVVRKKADLVIEGDLSRNEAR
                     NRHELSYTIKNIGEDTAREFQLQLKGYRSEDDLDPVVRDFDAIRLVAPGASRTGAGIG
                     ISSRFVRIELIVDPDDIVDEETHANNTLVLTVPSVQDDSTGAAQQTPTPEP"
     gene            1422..2696
                     /locus_tag="PKH07_06375"
     CDS             1422..2696
                     /locus_tag="PKH07_06375"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNT34603.1"
                     /translation="MMRFEYFIGWRYMLARSQVKFISVISLISISGVAVGVMSLIVVI
                     SVMNGFDRDLRERILGTKSHMVINDFYGITNYMELAKEIEREPNVVAAAPSVIGQGML
                     KYYNAALGVVAKGLDPNMEDRVTRLRENIVAGSDELLVEDVQKAQARVTDPSRTVETV
                     PIGEIKPHYSGIILGKEVAKQLFGLTTPPEDNHKEIEKEMLRSVIGKRLKFISFAEED
                     TPAGRVPRTETLEVAGVFDSGLYDYDMSLVFLALPTAQRLYDRKDKAMQIEMRLKDLN
                     KVDETAVLLQQRLQDRFDRLYGFVTWKEMNSVFFRALEIEKLAMFVILVLIVLVAAFN
                     IASTLIMVVMEKTKEIGILMSMGAKPGSIMRIFLFEGGVVASVGTLLGSIAGVLICLF
                     LRVYEIKMPGEAISTTSSGCPSRCWQVISSSL"
     gene            2781..>3237
                     /locus_tag="PKH07_06380"
     CDS             2781..>3237
                     /locus_tag="PKH07_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014160735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNT34604.1"
                     /translation="MSELLRVRGLCKTYQHGTNTLTVLKDLTFNLPAGDFVAIVGASG
                     SGKSTLLHLLGTLDRPTSGEITLAGQDYSQLDTAQLAEVRNRKIGFVFQFHHLLPEFT
                     ALENVLIPSLITGSDRKKARQHAEFLLECLGLADRLKHKPSELSGGEQQR"
BASE COUNT          764 a          825 c          910 g          738 t
ORIGIN      
        1 cgaggaactg ctcaccgcgg agagtggggg tgtgatcggg ctgttcgctt caacaggaag
       61 gggctgccca ccacaccatg aaatctatct gcgcgccctt catgagtgtc tgttcgttcg
      121 ggggatcaat tcaatcgggg cgctctccgc gatgggcaag attctgttcc agacgcagga
      181 caaagcgacg gatccgatct tcatgtttca gatctttggc gatccgctga ctcgcctccg
      241 tttgccggaa gtcaagggac acttggcggt caacccttca caaatccaaa gccgctatgg
      301 tggaagggtg cttgtgtccg aacaacccgg gctctcgctc caggggaaac agtatgcctt
      361 catcgcaaac gcacgtggtg agttgatccc agaaccgaag atgctgtccc atggttcggc
      421 ggatctgccg gtgggctttg acgtgccagc cgatctcgct ccgggaacct gttcggcaac
      481 aatattctct gcgtccatgt caccgacaca gccggcaacc gtcctgctcg gccagttcga
      541 gtgtctgcca cgagagccgc tgcacgagga agcactccct tcagaggcca gacccaacct
      601 gcggatcgaa accgccgata tcagtttcag cgatttcagc ccagtcaatg gacagaccgt
      661 cttcatcacg gttcgggttc acaacaccgg acatcgctca gttcgagatg ttgaggtcag
      721 agcctatgac ggggatcccc agacaggggg actggtgctc aacgatgatg tccaatggcc
      781 tttcagaact atcgcattga tcaggcccgg ggaatcagag accatccgac ttcgctggga
      841 tccttttcag aatgcgggcg aacacagaat ctgggtgcag ctggatcctt ccaacctgat
      901 cgaagaggaa aacaaacagg ataatctggc ttcgagaccc attgtcgtaa gaaagaaggc
      961 tgaccttgta atcgaaggtg atttgtcgcg caacgaagcc aggaatcgtc acgagctgag
     1021 ttacacaatc aagaatatcg gtgaagacac agcaagggaa ttccagttgc agctgaaagg
     1081 ctatcgttcg gaagatgatc ttgatccggt cgtgcgggat ttcgatgcca tccgtctggt
     1141 ggcacctggt gcaagtcgga cgggagcggg aatcggtatc tcgagtagat tcgtcagaat
     1201 cgaacttatc gtggatcccg atgacattgt ggacgaggag acgcatgcca acaacacact
     1261 cgttctgacg gttccttcgg ttcaagatga ttccacgggc gcggcgcagc aaacgccaac
     1321 gcctgagcct tgatgcagat caacgatgca ccatactcat tgacaggcat cggctgttgg
     1381 ggttactctt cgtcctgttc taaagaaaaa gttggcggtt aatgatgcga tttgagtatt
     1441 ttatcggctg gcgctacatg ctggccagaa gccaggtcaa gttcatctca gtcatctctc
     1501 taatatccat cagcggtgtc gcggtaggag tcatgtcgct catcgtggtc atctcagtga
     1561 tgaatggatt tgaccgcgac ctgagagagc ggattctcgg aaccaaatcc cacatggtga
     1621 tcaatgattt ctacggaatc accaactaca tggagttggc gaaagagatc gaaagagagc
     1681 ccaatgtcgt tgcagcggct ccctctgtga tcggtcaggg aatgctcaag tactacaacg
     1741 cggctctggg agtggttgcc aaaggtctcg atcccaacat ggaagatcga gttactcgat
     1801 tgcgtgagaa cattgttgct ggctctgacg aactgctcgt tgaggacgtt cagaaagccc
     1861 aggcacgagt taccgaccca agccgaaccg tggagactgt tccaatcgga gagatcaagc
     1921 cccactactc gggtatcatc ctgggcaaag aggttgccaa acagttattt gggctgacca
     1981 caccgccgga ggacaatcac aaggaaattg agaaggagat gcttcgatcg gtcatcggaa
     2041 agagactcaa gttcatctcc tttgctgagg aagatacgcc tgcaggcagg gttcctcgca
     2101 cggaaacatt ggaggtggct ggcgtattcg attcgggctt gtacgattat gacatgagtc
     2161 tggtgttcct cgcacttccc acagcacaac gtctctatga tcgaaaagac aaagccatgc
     2221 agatcgagat gcgactgaag gatctgaaca aggttgatga aaccgcagtg ctccttcaac
     2281 aacgtcttca ggatcgtttc gatcggctct acggctttgt cacctggaag gaaatgaata
     2341 gcgtcttttt tcgcgccttg gaaatcgaga aactggcaat gttcgtcatc ctggttctca
     2401 tcgtgcttgt ggcggcgttc aacatcgcga gcacgctcat catggtggtc atggagaaga
     2461 ccaaagaaat cggaattctg atgtccatgg gcgccaagcc cggcagcatc atgcggattt
     2521 ttctttttga aggcggtgtg gttgcctctg tgggaacatt gcttggctcg attgctggag
     2581 tgctcatctg tctgttcttg agggtgtacg agatcaaaat gccgggggag gcaatatcta
     2641 ctacatcgag cggttgcccg tccagatgct ggcaagtgat ttcatcatca ttataggaac
     2701 gaccatcctc gtcagtttgc tggctgccat ctacccagcg tggcaagcgt cacgtctcgt
     2761 tccaacggag gcgctacgct atgagtgagc ttcttcgggt cagggggctg tgcaagacat
     2821 accaacacgg aaccaacacg ttgaccgttt tgaaggattt gaccttcaac ctcccggcgg
     2881 gagattttgt cgcgattgtt ggagcgtcgg gttccggcaa gagcacgctg ctacatctgt
     2941 tgggaacact ggatcgtccg acgtccggag agattacgct ggcgggtcaa gattactctc
     3001 aactggacac tgcccagttg gcagaagtac gcaacaggaa gatcggcttc gtctttcagt
     3061 ttcatcactt gctgccggag ttcaccgctt tggagaatgt tctgatcccc agtctcatca
     3121 cgggtagcga tcggaagaaa gccagacaac acgccgagtt tctcctggag tgcctgggtc
     3181 tggctgaccg gctgaagcac aaaccgtcag aactgtcggg tggagagcag cagaggg
//
LOCUS       DAOQUC010000299         3234 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_34306_length_3234_cov_4.620006, whole genome shotgun sequence.
ACCESSION   DAOQUC010000299 DAOQUC010000000
VERSION     DAOQUC010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3234)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3234)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3234
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34306_length_3234_cov_4.620006"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            354..1241
                     /gene="cysK"
                     /locus_tag="PKH07_06385"
     CDS             354..1241
                     /gene="cysK"
                     /locus_tag="PKH07_06385"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004850316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HNT34605.1"
                     /translation="MWTSCVLDLIGNTPLIRLGNEPVFAKAEFLNPGGSIKDRVARAM
                     IEGAEKRGALVAGATIMEPTSGNTGIGVALVGRLKGYKVRIVMPEGMSEERKKIIRAL
                     GAELVLTPDEEGIAGAVEFVRRAAAADPTIFVAQQFENPDNPRVHYEQTARELWEQIE
                     GDVDCFVAGVGSGGTLQGIGKFLREHKPDIKIVAVEPKNVSALLGHEPGLHQIQGIGD
                     GFVPAVLDVSLIDEVLEVTDDVAIQTTRDLACEYGLLVGISSGANVWAARQLASRIQG
                     KIATILPDRAERYFSTALL"
     gene            complement(1251..2285)
                     /locus_tag="PKH07_06390"
     CDS             complement(1251..2285)
                     /locus_tag="PKH07_06390"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNT34606.1"
                     /translation="MGITIKDIARMANTSIATVSNVIGDKPGVGKAKKKQVLEIIKRT
                     GYRPHRSARSLVLQKSAMLGFVVSDLSNAVYVNVFRGVESVFRKRGYQVFLADSEMDG
                     AKERDNIELMLDNRVEALVIIPVHEFKLGDDVVHLIKLHQEGVPFVVMGRLENFEGDF
                     VTSEETRSAARMAQHLMGLGHTRIAFVGEDPDNRAAVERYLGVKRAVESDSRAELVTV
                     RCPANSSVRRWASTVDSVLRMPERPTALIMVNDITAITAIQRCKAVGLGVPEDISVVG
                     FGNLMTASVIEPALTTFAENVPGIVNKIVEALTRKIADPQCESIQSLVPQKMLRRKSS
                     APPPRRIKIL"
     gene            2579..>3234
                     /locus_tag="PKH07_06395"
     CDS             2579..>3234
                     /locus_tag="PKH07_06395"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HNT34607.1"
                     /translation="MVTEQNPNAVVLKAFSCLAQTASGIEGRPVLDTLVRELARCLGM
                     KWCFVCRLHSESAHHAETVSFWTEDSWRDNTDLQIADTPLAEVLVLGKRLILNGVGCQ
                     YGSLENLGITAAESFIGMGIKDAEGLPLGLICALDDKPVEPPDCVFDMLSLFADRAAA
                     ELMRLRAEHLLQKTQQELERSVRSRVEFLSTVSHELLTPLNAVIGFSEMLLSGRVGGL
                     T"
BASE COUNT          685 a          824 c          943 g          782 t
ORIGIN      
        1 atcttggtag caggtcagta cctgcggcgc gcgggctacg ctgactccct tggacgctta
       61 ctcggcaaat gactcgcttc tgctggactg agtgtcattg ggaggcagga tgacacttgg
      121 gcgggtttgc gtgaacctcg cttgacgaac cgattgaaat cggggattgt cttgttcggt
      181 ttgctttttg gtgcagagac gactttgtct gctgacacga aaagagcgtg agttctcatg
      241 ccagctgata cggcgcattc gtgtgggcta cgcccactct tacaaacgcg tgcgcattgg
      301 aagaaacaga cttcttggaa gtctcgtttc ctgggagtcc aggagggaga aatatgtgga
      361 cgagctgcgt tcttgatttg atcggaaaca ctccgttgat ccgtctcggg aatgaacctg
      421 ttttcgcaaa agccgagttt ctgaatcctg ggggcagtat caaggatcgg gttgcccgtg
      481 cgatgattga gggtgccgaa aagcgaggcg ctctggttgc aggtgcgacg attatggagc
      541 caacatccgg caacacggga atcggagttg ccttggtggg gaggctcaaa ggttacaaag
      601 ttcgtattgt gatgcctgaa ggcatgagtg aagagcgaaa gaagatcatt cgtgccctgg
      661 gggcggaact ggtcctgacg ccggacgaag agggcattgc cggtgcggtg gagttcgtgc
      721 ggcgtgccgc ggctgccgat ccgactattt tcgttgcgca gcagttcgag aatcccgaca
      781 atccacgcgt tcactatgaa caaacggcca gggaactctg ggaacagatc gaaggtgacg
      841 tggactgctt tgtggcgggt gtagggagtg gtggtacgct tcaggggatc gggaaattcc
      901 tgagagagca caagcccgac atcaaaatcg tcgctgtgga acccaagaac gtttcggcac
      961 tgttggggca tgaaccaggt cttcatcaga ttcagggaat cggagacggc tttgttccgg
     1021 cggttctcga tgtctccctg atcgacgaag tgctggaagt gaccgatgat gtggcgattc
     1081 aaaccacccg agacctcgcc tgtgaatacg gacttctggt tgggatttca tcgggcgcca
     1141 atgtgtgggc tgccagacaa ttggcttctc ggattcaggg aaagatcgcc acgattctac
     1201 ctgaccgtgc cgaaaggtat ttcagtactg cgcttctgtg atcttctcca tcacaggatc
     1261 ttgatgcgcc gagggggtgg agccgaggac ttgcgtcgaa gcatcttctg tggcaccagg
     1321 ctttgaatgc tctcgcattg tgggtcggca atcttgcggg tcaacgcttc cacgatcttg
     1381 ttgacgatgc ccggtacatt ctcggcgaaa gtggtgagtg caggctcaat gacactggca
     1441 gtcatcagat tgccaaatcc gacaacggag atgtcttcag gcacgcccag cccaaccgct
     1501 ttgcagcgct gaatggcagt gatggcagtg atgtcattga ccatgatcaa ggctgtcgga
     1561 cgttcaggca tgcgcagcac gctgtccact gttgaggccc agcgacgcac ggacgagttg
     1621 gcaggacatc gaacagtcac caactccgcc cgactgtcag actctacggc tcttttcaca
     1681 cccaggtagc gctccaccgc ggctcggttg tcgggatcct cccccacaaa agcgatgcgt
     1741 gtgtgtccca agcccatgag gtgttgagcc attcgcgcag cggaccgcgt ctcttcgctg
     1801 gtcacaaagt ctccctcaaa attctccagt ctgcccatca caacaaaggg aacgccttcc
     1861 tgatgcaatt tgatcaagtg aaccacatcg tcgcccagtt tgaattcgtg aacggggatg
     1921 atgaccagcg cctcaacacg gttgtcgagc atcagttcga tgttgtctct ctccttggca
     1981 ccatccatct cggagtctgc cagaaagact tgatagccac gcttgcgaaa gactgactcc
     2041 acgccccgaa acacgttcac atagacggcg ttgctcaagt cagacaccac aaaaccgagc
     2101 atggcgcttt tctggagaac cagacttcgg gcacttcggt gaggtcggta gcccgtgcgc
     2161 ttgataatct ccaaaacctg ctttttcttt gcctttccaa ctccgggttt gtccccgatg
     2221 acattggaga ctgtcgcgat ggatgtgttt gccatgcgag caatgtcttt gattgtaatt
     2281 cccattgtcg tccttcaggt cagaaatatg actggagccg tcttaccttt tgattccaac
     2341 agaggcggtt cccactcaca aggctagtat tcgtcctttg gcgtatccgc gtcaagtatt
     2401 ttcctctggg ataggattca attctggatt gccacaaagc aggtaaatgc ggataatgca
     2461 cgcatggttc aggaatgcgt tcgcagggtg ctgtgtttct gattcctaga cggacaaaca
     2521 ggtccgagac gtatcctcct gatgcgtgag tgccttgcgg atacttggcg gagagggcat
     2581 ggtgacagaa cagaatccca atgcggttgt gctgaaggct ttttcctgtc ttgcacaaac
     2641 cgcttcaggc atcgagggca ggccggttct ggacacgctt gttcgggaac tggctcggtg
     2701 tttggggatg aagtggtgct ttgtgtgccg actgcactct gaatctgcgc accacgctga
     2761 gacggtttcc ttctggacgg aagactcctg gcgagacaac accgatctgc aaatcgccga
     2821 tacgcctctc gcggaagtac tggtgttggg caagaggctt attctcaatg gagtcggttg
     2881 tcagtatggt tcgttggaga atctcgggat caccgcggca gagtccttta tcggtatggg
     2941 catcaaagat gccgaaggcc ttccgctcgg tctcatctgc gctttggacg acaaacccgt
     3001 cgagcccccc gattgtgtct ttgacatgct ctcacttttt gcagatcggg cggcagccga
     3061 gttgatgcgt ttgagagcgg agcatcttct tcagaaaaca caacaagaac tggagcgctc
     3121 cgtgcggtct cgtgtggaat tcctttctac ggtcagccat gagttgttga caccgctgaa
     3181 tgccgtgatc ggattctcag aaatgctctt gagtggtcgc gtgggcggcc tgac
//
LOCUS       DAOQUC010000300         3220 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_34546_length_3220_cov_2.792417, whole genome shotgun sequence.
ACCESSION   DAOQUC010000300 DAOQUC010000000
VERSION     DAOQUC010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3220
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34546_length_3220_cov_2.792417"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            3..899
                     /locus_tag="PKH07_06400"
     CDS             3..899
                     /locus_tag="PKH07_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014553408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HNT34608.1"
                     /translation="MTTRKAMLAVLLALLPAIVASGIVFGYRALALIVVCGAVCVITE
                     AAFQYFTGRKVCIQDGSALLSGVLLALILPANLPFYQAVVGSAFAMLFGKHLFGGLGR
                     NIWNPALLGRAFLMAAYPVSMTNYPRLPDAVTQATPLAAMKFNQEPTSLLSLFFGNVS
                     GSLGETSAVALIIGGVLLIWLRYADWRIPTGMLGAVIAITGANWVIRQGQVASPLFHL
                     LSGGLLIGAFFMATDPVTTPVTKLGRWIFGLGVGFLVALIRLKGGYPEGVMFAILLMN
                     SVRPLLDRLTVPKTFGQKRVAK"
     gene            920..1633
                     /locus_tag="PKH07_06405"
     CDS             920..1633
                     /locus_tag="PKH07_06405"
                     /inference="COORDINATES: protein motif:HMM:NF016121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HNT34609.1"
                     /translation="MKKILHMVAVLGLTGLISGWSLAYVYQVTKPVIEANDAARLENA
                     LHDVLPQATSFDRVDIAEGVYYVGKDGSGTVVGYATPVQGNGYQGAIRMILGIDIRLE
                     TMTGLVIFPNDETPGLGNRILEKPWRDQFAGLSPYPQVNYVKGRSREKPNEIQAITGA
                     TISSRSVVSAVNKGLTVLLREVGGTNPDQASQFGAFQCEDCPKWMNRAGQSSGTTPKE
                     PQPAAAHQPAATSESGGEQ"
     gene            1630..2253
                     /gene="rsxE"
                     /locus_tag="PKH07_06410"
     CDS             1630..2253
                     /gene="rsxE"
                     /locus_tag="PKH07_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013277525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HNT34610.1"
                     /translation="MSGTGRPLYREFIKGIWQENPVLVLVLGTCPTLAVTTSVLSGFS
                     MGMAATFVLVCSGVALSPIRKLIPDEVRIATYTAIIALFVTVAKMVLDARFPEISAKL
                     GPYIPLIVVNCIILGRMEAFCNKNNMIRSFLDTLGMGVGFTLTLLVLGAVRELLGSGT
                     VLGGTPWETTIYGETIKPMVVMLLPAGAFIMFGLLVGAYRALNPAKR"
     gene            2260..2841
                     /locus_tag="PKH07_06415"
     CDS             2260..2841
                     /locus_tag="PKH07_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013113074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit A"
                     /protein_id="HNT34611.1"
                     /translation="MGAAILVFIGAAITNNLVLVYFLGICPYLGVSNKLSSALGMATA
                     TTFVMTLTAPICWLVYHRLLIAHNLPFLQYVVFIIVIASLVQLVEMVLRKYVPVLYRA
                     LGIYLPLITTNCAVLGLALFMVLREYTFLESLFFGFGTGCGFSLALLIMAGIREEIQD
                     ADIPEPLKGVGITLISAGLLALAFMGFSGMVSA"
     gene            2879..>3220
                     /locus_tag="PKH07_06420"
     CDS             2879..>3220
                     /locus_tag="PKH07_06420"
                     /inference="COORDINATES: protein motif:HMM:TIGR01944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HNT34612.1"
                     /translation="MSVVVLASLSMFAIAAIFAVCLGLANLKLQVQKDARVVKARESL
                     AGVDCGGCGYPSCNGYAEAVVFDGASVTLCSPGGAETVKALADLMGIEPPVVSNRVAV
                     VHCGATKLTRKR"
BASE COUNT          633 a          811 c          917 g          859 t
ORIGIN      
        1 gtttgacgac tcgaaaggcg atgctggcag ttctcctggc actgctgccg gcgatcgtgg
       61 cttcggggat tgtttttgga tatcgtgcct tggctttgat cgtcgtctgt ggtgcggtat
      121 gtgtgatcac ggaagcggca ttccagtact tcaccggcag gaaagtgtgt attcaggatg
      181 gcagcgcatt gttgtccggt gtccttttgg cattgattct tccagccaat ctgccctttt
      241 accaggctgt tgttggttcg gcttttgcaa tgttgttcgg caagcacctc tttggtggac
      301 tggggcgcaa catctggaat ccggcacttt tgggcagggc atttctgatg gctgcgtatc
      361 cggtctccat gaccaactac ccaaggttgc ctgatgcggt cactcaggcg acgcctcttg
      421 cggcgatgaa attcaaccag gaaccgacct ctctcctgag tctcttcttc gggaacgtct
      481 ccggatctct tggagaaaca tctgccgtgg cgctcatcat cggcggtgtt ctgttgatct
      541 ggcttcggta cgcggactgg cgcattccca ctgggatgct cggtgccgtg atcgccatca
      601 ctggagccaa ctgggtgatt cgtcagggtc aggtggcctc acctctgttt catctgctga
      661 gcggtggact cctcattggg gcgttcttca tggcgaccga tcctgtgaca acacctgtga
      721 cgaaactggg acgatggatt tttggtctcg gtgttggttt cctggtggct ttgattcgcc
      781 tcaaaggggg ctatcccgaa ggagtcatgt tcgccatttt gctcatgaat tctgtgcgtc
      841 ctttactgga caggttgacg gtgcccaaga cctttggtca gaaaagggtc gccaagtgat
      901 ttgtttctgg tggtgctaga tgaagaagat tcttcacatg gttgcagttt tgggactgac
      961 agggctgatc agcggttggt cgcttgccta cgtctaccag gtcaccaagc ctgtcattga
     1021 ggccaatgat gcagcacgac tcgaaaatgc tctgcatgat gtacttcccc aggccacttc
     1081 cttcgacaga gtggacatcg ctgaaggggt ctattatgtc ggcaaagacg gttctggaac
     1141 tgtggttggc tacgccaccc cagttcaggg caatggctat cagggcgcga tccggatgat
     1201 tctgggtatt gacatccgcc tggagacaat gactggcctc gtgatctttc ccaatgatga
     1261 gactccaggc cttggcaacc gaattctcga gaagccatgg cgcgatcagt ttgccggact
     1321 gtctccctat ccacaagtca actacgtcaa aggtcgctcg cgtgagaagc cgaacgaaat
     1381 ccaggcgatt accggcgcga ccatctccag ccgcagtgtt gtcagtgcag tcaacaaggg
     1441 actgaccgtg cttctccgtg aagtaggcgg aacaaaccct gatcaggctt cccagtttgg
     1501 ggcatttcaa tgtgaggact gtcccaagtg gatgaacagg gcagggcaat cctctggaac
     1561 aacgccgaaa gagccgcagc cggcagcagc acatcagcct gctgcgactt ccgaatcagg
     1621 aggcgaacaa tgagcggcac cggcagaccg ctttaccgtg aattcatcaa aggaatctgg
     1681 caggagaatc ctgttttggt gctcgtgctg ggaacctgcc ctacgcttgc cgtaacgact
     1741 tccgttctca gtggtttctc catgggtatg gctgccacct tcgtcctcgt gtgttcggga
     1801 gtggctttgt cacccatccg aaaactcatc ccagatgagg ttcgtattgc cacatacact
     1861 gcgatcattg ctctttttgt tactgttgcc aaaatggttc tggacgccag atttcctgaa
     1921 atcagtgcaa aactgggacc ttacatcccg ctcatcgtcg tcaactgcat tatcctcgga
     1981 agaatggaag cattctgcaa caagaacaat atgatccgat cgtttctcga tacactcgga
     2041 atgggtgttg ggttcacgtt gacgctcctg gttcttggtg cggtgcgtga attgcttggc
     2101 tcaggcacgg tgctgggtgg gacaccgtgg gaaaccacaa tctatggtga gacgattaag
     2161 cccatggtgg tgatgcttct gcctgcaggt gcattcatca tgtttgggct tctcgttggt
     2221 gcgtatcggg cgctcaaccc ggcaaagagg taagacaaaa tgggtgcagc gattctcgtt
     2281 ttcattggtg cggcgatcac caacaacctg gtgcttgtct actttctggg tatatgtccc
     2341 tatctgggcg tctccaacaa actttcatcg gcacttggga tggccacggc gacaaccttt
     2401 gtcatgactt tgacggctcc gatctgttgg ttggtgtacc accgacttct tattgcccac
     2461 aacctgccat ttttgcagta tgtcgtcttt atcatcgtca tcgcatctct ggtgcagctc
     2521 gtcgagatgg ttcttagaaa atacgtgccg gtcttgtacc gcgcactggg tatctatctt
     2581 cctttgatca caaccaactg cgccgttttg ggcttggctc tgttcatggt gctgcgggaa
     2641 tacacctttc tggaaagcct gtttttcggt tttggcacag ggtgtggttt ctctctggca
     2701 ctcctgatca tggcgggcat tcgagaggaa attcaggacg cagatattcc cgaaccactc
     2761 aaaggagttg gcatcacgct tatcagcgcc ggtcttttgg ctctggcgtt tatgggattc
     2821 tctggaatgg tttccgctta gcaggaaaag gaccgaagcg gaaggttaag gaaaagtcat
     2881 gtccgttgtc gtcttagcgt cattgagcat gtttgccatc gcggccattt ttgctgtctg
     2941 tctcggtttg gcaaacttga aactgcaggt gcagaaggat gcacgagtcg tgaaggcacg
     3001 cgaatctctt gccggagtgg actgtggggg atgtgggtat cccagctgca acgggtacgc
     3061 tgaagccgtg gtttttgatg gggcttcggt taccctgtgc agccctggcg gagcggagac
     3121 cgtgaaagcc ttggcggact tgatgggtat cgaaccgccc gtggtgagca atcgagttgc
     3181 cgtggtgcat tgcggtgcaa caaaactgac tcgaaagcgg
//
LOCUS       DAOQUC010000301         3215 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_34623_length_3215_cov_2.580380, whole genome shotgun sequence.
ACCESSION   DAOQUC010000301 DAOQUC010000000
VERSION     DAOQUC010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3215
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34623_length_3215_cov_2.580380"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1379
                     /locus_tag="PKH07_06425"
     CDS             <1..1379
                     /locus_tag="PKH07_06425"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HNT34613.1"
                     /translation="AAQQSLTTVTGGLVLSIKVLGELVGFGFLVCLSNFCSACESALT
                     CLGRTGRESIKDRDLGKWDEEPYRYLVTVMVGNVLSIIGAALMAQAVAFRYFSFINIP
                     NAFLLACLTSFFATAFSVFYVGEVLARTLGKKYALRIAPKAIKALDLLYYSILWLPTR
                     LVLVLTGKMFQIAGIPGQPAQIIRTEEELRNLLHTGSQDTELEQEQSEMIHSIFEFGE
                     TVAREVMVPRTDIDCVSEDATLQELLHCVTDCGHSRIPIYREKTDNIVGILYAKDLLR
                     YWQIRQDAQQQDSQVQNDPFRIEQFMRPPHFVPENKSLRDLLKDFRSKRMHLAIVVDE
                     YGGTAGLITIEDVLEEIVGEIQDEYDSEEELYSIQEDGSILIDAKMSAYEITEQLGIH
                     LTGEDFETLGGFLFSVIGDVPTVGREVLHDDLRITVVEADERHVIKARIQRLTKPTPS
                     TESEGTPQ"
     gene            1376..2074
                     /locus_tag="PKH07_06430"
     CDS             1376..2074
                     /locus_tag="PKH07_06430"
                     /inference="COORDINATES: protein motif:HMM:NF016270.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HNT34614.1"
                     /translation="MKHVPFTKGSTLKTLRGHFFAGLVALAPLFITVWMLLTIFNYLD
                     ARIRGYFMELFNLQVPPYGAGVLVTFLGIVFVGMVARNFIGGRILQFFEGILLKVPVA
                     NRLYGAVKQIIGAFVGRDKTVFEKVVLVQYPRQGCYCLGFLTYPEQIRFRGHDNLPEL
                     LCIFVPTTPNPTSGMLLLFPEEQVQILSINIEDGLKMIISGGVVLPESVAPCGEPGSL
                     SVQDGKRYDEKPNA"
     gene            2107..2448
                     /locus_tag="PKH07_06435"
     CDS             2107..2448
                     /locus_tag="PKH07_06435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34615.1"
                     /translation="MKSVILLICVVLLALCPCILEGQADDHEHDCKTHPQDQLSVGLC
                     CGDCCENARMCRSHMDAHMDDQETLPQPLRESVSLPIFSALSRFCPSEKQDELSRFGL
                     LDLTGIVVLRC"
     gene            2606..>3215
                     /locus_tag="PKH07_06440"
     CDS             2606..>3215
                     /locus_tag="PKH07_06440"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNT34616.1"
                     /translation="MWIYRRTSMLMTVWVLLVSTAQPATLEQVLERMRSGNPAFETAQ
                     AAVEVSKGYHRQAGLRSNPSLSVENEEMSLSRFGSLGRASTTLALSQPFDSWNKRSLN
                     QLTTAKDVALARLETRALELELSSLVCQTFSLILVSEEREHLLKKFLEQSQRVLETVT
                     EQVLGGERPIMEQLRVQAELHNIEVRQQQDEAEQQRLILQLAA"
BASE COUNT          723 a          840 c          858 g          794 t
ORIGIN      
        1 cggcggcaca acagtcgctg acaactgtca cgggaggatt ggttttgagc attaaggttc
       61 tcggcgaatt ggtggggttt ggctttctcg tatgcctctc gaatttctgt tctgcgtgcg
      121 agagcgctct cacctgcctg ggcagaacgg gcagggagag catcaaggac agagacctgg
      181 gcaagtggga tgaggaaccc tatcgctatc tcgtgacggt catggtcgga aatgtcctct
      241 caatcatcgg cgccgctttg atggctcagg cggtggcttt tcgctatttc tcgttcatca
      301 acattcccaa cgcttttctc ctggcttgcc tgaccagttt cttcgccacg gcgttctccg
      361 tattctatgt gggcgaggtt ctcgccagaa ccctcggcaa gaagtatgct cttaggatcg
      421 cccccaaggc cattaaggct ctcgatctgc tctattacag cattctttgg cttcccaccc
      481 gcctggtgct tgtgctcacc ggtaagatgt ttcagatcgc gggaatccct gggcaaccgg
      541 cacaaatcat ccgaacggag gaagaactcc gaaatctgct ccacacgggc tcccaggata
      601 ccgagttgga gcaggaacaa agcgagatga tccacagcat cttcgagttc ggcgaaaccg
      661 tggcacgcga ggtcatggtt ccccgaaccg atattgactg tgtcagtgag gacgccacgc
      721 ttcaggaact tctgcactgt gtcactgatt gcggacactc acgtatcccc atttacaggg
      781 agaaaacgga caacattgta ggtattctct atgccaagga tcttctccga tactggcaga
      841 tacgtcagga cgcccaacag caagacagcc aggtgcagaa tgacccgttc cgcatcgagc
      901 agttcatgcg tccgcctcat tttgttccag agaacaagtc cctcagagat ctcttgaagg
      961 atttccgatc caagcgaatg catctcgcta tcgttgtaga tgagtatggt ggcacagccg
     1021 gtttgattac gatcgaggac gtgcttgagg aaattgtcgg cgaaatacag gatgagtatg
     1081 attctgaaga ggaactgtac tcaatacagg aagacggatc gatcctcata gatgccaaga
     1141 tgtccgccta tgagatcacc gagcaactgg gaattcacct gactggcgag gattttgaga
     1201 cgctgggagg attcctcttt tctgtcatcg gtgacgttcc caccgtggga cgtgaagtgc
     1261 tccatgacga tctccgaatc acggttgtcg aagcggacga gcgacatgtc atcaaggctc
     1321 gcatccaaag actcaccaaa ccgactcctt caacagaatc ggaaggaacc ccacaatgaa
     1381 acatgttccc ttcacaaaag gctctacgct gaagaccctg agagggcatt tcttcgcagg
     1441 cctggtggct ctggcacctc tcttcatcac cgtttggatg ttgctcacaa tcttcaacta
     1501 tctggacgcg agaattcgtg ggtatttcat ggagcttttc aatctccagg ttccacccta
     1561 tggggcaggc gtccttgtca cttttctggg gattgttttt gtcggaatgg tggcacgcaa
     1621 tttcatcggg ggaagaattc tgcagttctt tgaagggatt cttctgaaag tacctgttgc
     1681 caatcgtctc tacggagccg tcaaacagat catcggagca ttcgtcggtc gcgacaagac
     1741 cgtattcgag aaggttgtct tggttcaata ccccaggcag ggctgctact gtcttggttt
     1801 tctgacttac ccggaacaga tccgattccg tggtcacgac aatctgccag aactgctctg
     1861 tatctttgtt ccaacaacac ccaatccaac ctctggcatg cttctgctct tcccagagga
     1921 gcaagtccag attctctcca tcaacatcga ggatggcctg aagatgatta tctccggcgg
     1981 cgtggtctta ccggaaagcg tcgccccatg cggagagccg ggaagtctgt cagtacaaga
     2041 cgggaaacgt tacgatgaga aaccgaatgc gtaatttgta taggcttttt tgattgaggg
     2101 acaaggatga aatctgtaat cctgctgatt tgtgtcgtgc tcttggctct ttgtccctgt
     2161 attctggaag gtcaggcaga tgatcacgag cacgactgta aaacgcatcc gcaagatcag
     2221 ctgagtgttg gactgtgctg tggtgactgc tgtgagaacg ccagaatgtg ccgttcccac
     2281 atggatgcgc acatggatga ccaggaaacg cttccccagc ctctgcggga gagcgtctct
     2341 cttccgatct tttctgccct ttcacgcttc tgcccttctg aaaagcaaga cgaactgtct
     2401 cgattcggac tgctcgatct gacaggaatc gtcgtccttc gttgttgaac cacctaacta
     2461 ccggccggcg cacggactgc tttctcccgt gcctgccttc gctgagaaca gcattctctt
     2521 gatatcttct cttgcggggc tcctgtcggt aatcgttgtc gtctttttcc cgagtattga
     2581 aactggccag aaaggttgat gcaatatgtg gatatatcgt cgcacttcta tgctgatgac
     2641 cgtatgggtg ctgttggttt caacggcaca accggcgact ttggaacagg tgctggaaag
     2701 gatgcgttca ggcaatccgg cattcgagac ggcacaggcg gccgtggagg tttccaaggg
     2761 ttatcatcgc caggccggcc tgcgctccaa cccttcgttg tctgtggaga atgaggaaat
     2821 gtctttgagc cggttcggca gtctgggacg ggccagtaca acgttggcgt tgagtcagcc
     2881 gttcgattcc tggaacaaga gatccttgaa ccagttgaca acagccaagg atgtcgctct
     2941 cgctcgactg gagaccagag cccttgagtt ggagctcagt tccctggttt gtcagacgtt
     3001 ctccctgatc cttgtctcgg aagagagaga gcacctgctc aaaaagttcc tggagcagtc
     3061 tcagcgggta ctggaaaccg tcaccgaaca ggttctcggc ggagaacgtc caatcatgga
     3121 gcagctacgg gttcaggctg aactgcacaa catcgaggta aggcagcaac aggatgaagc
     3181 cgaacaacag cggctgatac tccagttggc agcct
//
LOCUS       DAOQUC010000302         3213 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_34659_length_3213_cov_2.714693, whole genome shotgun sequence.
ACCESSION   DAOQUC010000302 DAOQUC010000000
VERSION     DAOQUC010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3213
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34659_length_3213_cov_2.714693"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..756
                     /locus_tag="PKH07_06445"
     CDS             <1..756
                     /locus_tag="PKH07_06445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34617.1"
                     /translation="LVMAAYPGCSMAVYDPTQPWALGTNPRRTPGLSKLVYRPHEMCL
                     SPDGERVLIGGVPDYGLLGGAISIFNPETMKIEAEYRNIIPNQSITTLCLDGQGRVVA
                     GSCTQGGSGSHTTEKEAHLFLWDYDKREKAFDVVPVTGEGIIRAIRLGPDGLLYGLTH
                     NGHFFVFNSESLQIVHLEKLPYGRPTFHPMELAESGLFYVTFGNAIVEITPGTFHHRI
                     LADFPEAIHSGIALVKGRVYFSSQDHLLSYPLP"
     gene            753..2225
                     /locus_tag="PKH07_06450"
     CDS             753..2225
                     /locus_tag="PKH07_06450"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNT34618.1"
                     /translation="MRKKSQTFVHSLSAIWHRWSEHPWFAKGMFLLLFLLAIALRLYG
                     INSEVLWFDEIASVTKAGLPTLSEVSRKVLATETTPPLYYLLLHFWLKISASTEWIRL
                     SSTLMSLACLATTGLLAYRIKGTALALLSCFLMAISQYQIYYAQEARAYAGLMLICVV
                     AALLLSRCFDEPSRKRNWLFYAIALTVGMHFHYCFYFTLLGHAGYAAWNLSCRPIARR
                     QTWKMLLVIGLLAGVNLFFAARMFSLQRGEGLTDWIPRMSWGNFLGVLRSFSCGISAT
                     LSKHLINACVLIGLSMWTWFFCSKTVSVKWRGRLFWFAVFPMAFMIVLSFWIPVVFYG
                     TRYLSICQPFFVIALAGGFLAFPKRWNILLLSVLSVIWLMAVQNAVNTTQKRRYDLAA
                     EIIADQKHEGDSVLVIPNYSYECLNYFRPDIAPALDFPTSDLADRFEHIFGAGQRVWV
                     VSITPSETTQERMMQPFAERNMSVDMPRGPYLRVSRYVAK"
     gene            complement(2247..>3213)
                     /locus_tag="PKH07_06455"
     CDS             complement(2247..>3213)
                     /locus_tag="PKH07_06455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34619.1"
                     /translation="DDSGLCLTAQISGDLKVKRLYVPQENGFRVVTTLSNTGASRSDP
                     AILRSMTQWDLKDFRRWVLVLPGTQSPHSFEISTLFSSDEPIREFATAQFGSAWVLWN
                     ATSSIGVMGESDASVKTLVFELDKKRSVLKVNQRTGFHAFRKKGGWVTERKYQIIRST
                     PEGFSTEPKSPDLKRESSVVIEPDSFRLFRQGELSRIVTDRSAEGGVCGWLTGHTREW
                     AIQAEVPSGRLDPERKYQLVAHVKAVKKSDNGSAYTVGIWDHDSHRAVLSVTRDASKA
                     SGDWDEFVVGEFVPGKSFYIWICPEANPDAISEIRVARFVIRAAS"
BASE COUNT          700 a          885 c          805 g          823 t
ORIGIN      
        1 cttgtgatgg ccgcctatcc cggctgctcg atggctgttt acgatccgac acaaccctgg
       61 gcactgggca caaatcctcg aaggacacct gggctctcga aacttgtcta tcgcccccat
      121 gaaatgtgtc tttctccgga cggcgagcgt gttctcatcg gtggggttcc cgactacggt
      181 ctcctgggag gggcgatctc catcttcaat ccagagacga tgaagataga agcggagtat
      241 cgtaatatca ttccaaacca gagcattaca acactttgtt tggatggaca gggaagggtt
      301 gtcgcaggca gctgcactca gggaggctca gggagccaca ccacggaaaa ggaagcgcat
      361 ctctttctct gggactatga caaaagagag aaggccttcg atgtcgtccc tgtcaccggc
      421 gagggaatca ttcgcgccat caggctcggt cccgatggac tgctctatgg gttgactcac
      481 aacggacatt tcttcgtctt taactctgag agccttcaga tcgtccacct tgagaaactt
      541 ccctatggca ggccaacgtt ccatcccatg gaacttgcag aaagcggcct gttctatgtg
      601 accttcggca atgctattgt ggagatcacc ccaggaactt ttcatcatcg gattctggct
      661 gattttcccg aggccattca ttcgggtatc gctctggtca aaggcagagt ctatttcagt
      721 tcacaggatc atctgctcag ctatcccctg ccatgaggaa gaaatcccaa acctttgttc
      781 attcgctcag tgcgatttgg caccgttggt ctgagcatcc ttggttcgcc aaagggatgt
      841 tccttcttct cttccttctg gcgattgcac tccgactcta tgggatcaat tctgaggttc
      901 tctggtttga cgagatcgca tcggtgacca aggcaggcct gccgacattg tcagaggtct
      961 cccgaaaggt acttgccaca gagaccacgc cgcctttgta ctatctcctt ttgcatttct
     1021 ggctcaagat ctctgcaagc accgaatgga tcaggctttc atctactctc atgagccttg
     1081 catgtcttgc cacgacagga ctcctggctt accgcatcaa aggaaccgcg cttgcgctgc
     1141 tctcatgctt cttgatggcg atatcgcagt accagattta ctatgctcaa gaggcgcgtg
     1201 cgtacgcggg tctcatgctg atttgtgtcg tcgcagcact actcttgagc aggtgttttg
     1261 acgaaccctc tcgaaagcga aactggctct tctatgcaat tgccctcaca gtcggaatgc
     1321 acttccatta ttgtttctac ttcacacttc tcggacacgc gggctacgct gcctggaacc
     1381 tgtcgtgccg ccccattgcc cgaaggcaga cttggaagat gctcttggtc atcggtttgt
     1441 tggccggagt gaatctgttt ttcgcggcaa ggatgttttc tctccagcgg ggggaagggc
     1501 tgacggactg gattcccagg atgagttggg gcaactttct cggagtgctt cggtctttca
     1561 gttgtgggat ttcggccaca ctttcaaaac accttatcaa cgcatgcgtc ttgatcggtc
     1621 tttcgatgtg gacttggttt ttctgttcca agacagtttc cgtcaaatgg agaggaagac
     1681 ttttctggtt tgcggttttt cccatggcct tcatgattgt gctctcgttc tggattccgg
     1741 tggtttttta tggaacacgg tatctcagca tctgtcagcc gttttttgtg attgccctgg
     1801 caggtggctt tctggcgttt cccaaacgtt ggaacattct cttgttgtcg gtgctctcgg
     1861 tcatctggct gatggcggtt cagaatgcag tcaacacaac tcagaagcgg cgatatgatc
     1921 ttgctgcgga gatcattgcc gaccagaagc acgagggaga ctctgtcctc gtcattccaa
     1981 actactccta tgagtgtctc aattacttca ggccggacat cgctcccgca ttggactttc
     2041 cgacgagcga tcttgcggat cgtttcgagc acatctttgg tgctggacaa cgggtctggg
     2101 tcgtcagtat cactccgtcg gaaacaacac aagagcggat gatgcagcca tttgcagaac
     2161 gcaatatgtc ggttgatatg cctcgcgggc cctatcttag agtctccaga tacgtggcaa
     2221 agtgagagag ccgttcaaag catctctcag gatgcagctc gtatcacgaa cctggcaacg
     2281 cgaatctcgc tgattgcatc aggattggct tctggacaaa tccaaatata gaaggacttt
     2341 ccaggtacaa actcgccgac gacaaactcg tcccaatcgc ctgacgcttt tgatgcatcc
     2401 cgagtaacac tgagaaccgc tctatgagaa tcgtgatccc agattccaac cgtatacgcg
     2461 gagccgttgt ctgacttctt caccgccttg acgtgagcca cgagttgata cttcctctca
     2521 gggtcgagtc tgcccgaagg cacctccgcc tgaattgccc attcccgggt gtgaccagtc
     2581 aaccagccac acacgccacc ctcagcagat ctgtcggtga caatgcgact caattctccc
     2641 tgccgaaaca gtcggaaaga gtctggctcg atcaccacag acgattcacg cttcagatcc
     2701 ggcgacttcg gttctgtgga aaaaccctct ggcgtactgc ggatgatctg atatttgcgc
     2761 tcagtgaccc atcctccctt tttccgaaag gcgtgaaaac cggttctttg gttcaccttc
     2821 aggacacttc gcttcttgtc caactcaaac acaagcgtct tgaccgatgc gtctgactca
     2881 cccatgactc caatggatga agtggcattc cagaggaccc atgcagaacc gaactgcgcc
     2941 gtggcgaatt ctcgaatcgg ttcatcggac gagaagagtg ttgaaatctc aaaggagtgg
     3001 ggcgactgcg ttccaggcaa aaccaagacc caccttctga aatccttgag atcccattga
     3061 gtcatggaac gaaggatcgc cggatccgac cgagaggcac cggtgttgct cagcgtggtc
     3121 accacccgaa agccattctc ctggggaacg tagagccgct tcaccttcaa gtcaccagaa
     3181 atctgagccg taaggcacaa tcccgaatca tcc
//
LOCUS       DAOQUC010000303         3211 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_34686_length_3211_cov_3.521546, whole genome shotgun sequence.
ACCESSION   DAOQUC010000303 DAOQUC010000000
VERSION     DAOQUC010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3211
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34686_length_3211_cov_3.521546"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..536
                     /locus_tag="PKH07_06460"
     CDS             <1..536
                     /locus_tag="PKH07_06460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNT34620.1"
                     /translation="HKRFLHDDLQRQGFRKKLALVAETILKEVPGAAIASDQPYREYD
                     LGIDFREDVTPLAAEEVRLIKDIFKRMGAHAKISSIHVNGWFGDFDKLTTAKLWAQER
                     LGVDLEDARDRFAFCGDSPNDEPMFAFFPLSFGVANVRPFLDMIECHPALITTREHGA
                     GFCEITDLILQARTLAR"
     gene            complement(553..1593)
                     /locus_tag="PKH07_06465"
     CDS             complement(553..1593)
                     /locus_tag="PKH07_06465"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HNT34621.1"
                     /translation="MMNVLLILLSLCLSLSVFPVLCRAESPSSFSVTTSEELLDAVKS
                     VGGRGGIIRLAPGDYSISQPIEITNTNQLTFLGSGWSTVIRKKGAGDALRFVESHFCF
                     VKDLMIVGEIESASGSGIFFTDSSSCTVDSCRICTFPESGIRFAGRESRQMSSNSVNN
                     CHFISNKGDQLHSLYNNDFYIIGNQFGTHHGNPNSGCALNHSSAGTYSMNYHWGNKNA
                     FRLDGRCHFNRIENNRFEESVEAGMVLGATDADFNVFNIITGNTIHTNSKGNFGLFAA
                     VEAYKCTETTFCQNQILSWDSSSTRHKTGLAIGEGCVHWIVKDNILRHQTERALVYDE
                     NAGHIVKDNLTD"
     gene            complement(1715..>3211)
                     /locus_tag="PKH07_06470"
     CDS             complement(1715..>3211)
                     /locus_tag="PKH07_06470"
                     /inference="COORDINATES: protein motif:HMM:NF027452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HNT34622.1"
                     /translation="KAASQDLGDEGYGVATKGRRLFLWGGRTRGVINAVFALLEEDLD
                     CRWYTKDHFRIPKREHLVFSPVQRTYNPRLKLRDPFYFVSFDAEWSFRNRTNAPSALV
                     PEEWGGRMDYGEWFVHTFNALVPPAKYFAEHPEYFMLDAAGNRSTQQLCTTHPDVVRL
                     VIERVRDYLCRNPNVEIVSVSKNDGGQTCLCESCRALDDVEGTNMAALLHLVNKVAEA
                     VESDYPHVLVSTLAYLETVGVPKTMRPNKNVVIRLCNDTVGAWSHPFTPGEECQFGNL
                     MKAWSKAHDKISIWDYTVNFSHYMAPMPNMRVIAENIRFMVENNAIGIMTQGAYQSPG
                     GERDWMRSWVIGKLMWDPSRDLLELMRDFIWGHYGEAAAAIEEYNRLLEGQAETFKES
                     LAQPPGGIRYGMDHPFLSREFLNTANAIFDRAEKLAESEAILHRVQQDRLPIMYVELS
                     RGRELLGARYDGVLDRFEKIARRVGVTHLAEDWSGPDFESKIKAWRGH"
BASE COUNT          754 a          882 c          801 g          774 t
ORIGIN      
        1 tccacaagcg ctttctgcac gacgatttac agcgccaggg ctttcgcaaa aagctcgccc
       61 ttgtggcaga gacgattctg aaagaggtgc ctggcgccgc catcgcttca gatcaaccat
      121 accgggaata tgatctcggg atagattttc gggaggatgt tacacctctg gcagccgaag
      181 aggttcgctt gatcaaggat atcttcaagc gcatgggcgc ccatgcaaag atctcctcaa
      241 ttcatgtcaa cggctggttt ggtgatttcg acaaactcac aaccgccaaa ttgtgggcac
      301 aagagcgttt gggagtggac ttggaggatg cccgcgacag gttcgccttc tgtggcgact
      361 cccccaacga tgaaccgatg tttgccttct ttcccctgtc gtttggcgtt gcgaacgttc
      421 gacccttcct ggacatgatc gaatgtcatc cggcgctgat cacaacacgt gaacacggcg
      481 ccggattctg tgagatcacc gatctgattc ttcaggctcg cacgctggct cgttagcagt
      541 tcattgcttc gctcagtccg tcagattgtc cttgacgata tgacctgcgt tctcatcata
      601 taccagagcc ctttcggtct gatgccgaag gatgttgtct ttgacgatcc agtgtacgca
      661 tccttccccg atagcaagcc ccgttttgtg ccttgtcgaa ctggagtccc aactcagtat
      721 ttgattctgg cagaaggttg tctccgtaca tttatacgcc tcaacagccg caaacagtcc
      781 gaagtttccc ttggagtttg tgtgtatcgt attgcctgtg atgatattga agacgttaaa
      841 atcagcatcc gtagcgccca gaaccatacc tgcctcaacg ctctcttcaa aacgattgtt
      901 ctcgatgcgg ttgaaatgac agcgtccgtc aagacgaaag gcgttcttgt tgccccagtg
      961 atagttcatt gaataagtgc cggcggaaga atggttgagg gcacatccgg aattcggatt
     1021 tccgtgatgt gttccaaact gattgccgat gatgtagaag tcgttgttgt agaggctgtg
     1081 aagttggtcg cccttgttgc tgatgaagtg gcagttgttg acactattgg aggacatctg
     1141 gcgactctct cttcccgcaa accgaattcc gctctcagga aaggtacaaa tgcgacaaga
     1201 gtccacagtg caggaactgg agtcggtaaa gaagatgcca gagcccgaag cagattcgat
     1261 ctctccgacg atcatcaagt ccttgacaaa acagaaatgg ctctcaacaa aacgcagggc
     1321 atccccagca cctttctttc ggatgacagt gctccaaccg ctccccaaaa acgtcaattg
     1381 gttggtgttc gttatctcaa tgggctgaga aatggaatag tcacctggag caagtcgaat
     1441 aatccctccg cgccctccaa cagactttac cgcgtccaag agttcttcgc ttgtcgtgac
     1501 tgaaaacgac gacggcgact cggcacggca caggacgggg aacacggaca ggctgaggca
     1561 gagcgaaagc agtatcagca acacgttcat cataagactc ctgaatcgca ttatcggtac
     1621 cctccacaaa agaaagactt ccatccctga tatgatggca caaaggctgt gcgggcacag
     1681 tagaaaatgc cgcgagcgca ggaaaacgct tagatcaatg tccacgccat gccttgatct
     1741 ttgactcgaa atcgggtccg ctccagtctt cagcgagatg ggtcacacca actcgcctgg
     1801 caatcttttc aaaacggtcc agcactccat cgtatctcgc tccgagtaac tcccgtcctc
     1861 gggacaactc gacatacatg atcggaagcc tgtcctgctg cacgcggtgc aagatggcct
     1921 cactctcggc aagtttctca gcgcggtcga atatcgcgtt ggcagtattg agaaactccc
     1981 tggaaaggaa tggatgatcc atgccgtacc gaattccccc aggtggttga gccagtgatt
     2041 ccttgaacgt ctctgcttga ccttcgagca accgattgta ttcttcgatg gcggctgccg
     2101 cttccccata gtgtccccag atgaagtcac gcatcagttc gagaaggtct cgggaaggat
     2161 cccacatcag ttttccgatg acccatgagc gcatccagtc ccgctctccg ccggggcttt
     2221 gataggcacc ctgtgtcatg atcccgatgg cattgttttc gaccatgaac ctgatgttct
     2281 cggcaatcac tctcatgttg ggcattggtg ccatgtaatg gctgaagttc accgtgtagt
     2341 cccagatgga gattttgtcg tgagcctttg accacgcctt catcaggttg ccgaactggc
     2401 attcctcacc aggcgtgaag ggatggctcc acgcaccaac ggtatcattg cacaacctga
     2461 tcaccacatt cttgttcggt ctcattgtct tgggcacacc cacagtttca aggtaggcca
     2521 gagtggaaac cagtacatga ggataatcgg actcaaccgc ctcagcgacc ttgttgacca
     2581 gatggagtag agccgccata tttgtgcctt ccacatcatc gagcgctctg caactttcac
     2641 acaaacaggt ttgcccgcca tcgttcttgg aaacgctaac gatctcaaca ttgggattgc
     2701 ggcacagata gtctctcacc cgttcgataa ccaaccgaac cacatccgga tgagtcgtgc
     2761 atagttgttg ggtgctccga ttgcccgcag cgtcaagcat gaagtattcg ggatgctcag
     2821 cgaagtactt tgcaggcgga accaatgcgt tgaaggtatg cacaaaccac tctccatagt
     2881 ccatgcgtcc tccccattct tctggaacca gggcactggg agcgttcgtt ctgttgcgaa
     2941 aagaccactc cgcatcgaag gagacaaagt agaagggatc ccgcaatttc agcctcggat
     3001 tgtaggtacg ttgaactggt gagaagacaa gatgctcccg cttgggaatt cggaagtgat
     3061 ctttggtata ccagcgacag tcgagatcct cttccagcag cgcaaacact gcgttgatca
     3121 cgcctcgtgt tcttcctccc caaagaaaga gcctccgccc cttggtggcg actccatatc
     3181 cctcgtctcc caagtcctgg gacgcggctt t
//
LOCUS       DAOQUC010000304         3208 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_34734_length_3208_cov_2.979702, whole genome shotgun sequence.
ACCESSION   DAOQUC010000304 DAOQUC010000000
VERSION     DAOQUC010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3208
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34734_length_3208_cov_2.979702"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            23..>294
                     /locus_tag="PKH07_06475"
     CDS             23..>294
                     /locus_tag="PKH07_06475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34623.1"
                     /translation="MSVRVRDGRTPKARDLSAWISLSLRQLGFQPSSERRLCDEKALL
                     SSVDMIESADSLCAEGGLFTLEKVEVLSRTPEGDLVSLMDVPVPEAG"
     assembly_gap    295..304
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <305..569
                     /locus_tag="PKH07_06480"
     CDS             <305..569
                     /locus_tag="PKH07_06480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34624.1"
                     /translation="GWRRRVYTRGHVGYAHSRAVLAHRRDDSVLRRQAPAGVRAMPAP
                     TNACSLEELDFFEVWFPGSFILKEQVFTHGHSGKVQCDGQEWK"
     gene            566..2134
                     /locus_tag="PKH07_06485"
     CDS             566..2134
                     /locus_tag="PKH07_06485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HNT34625.1"
                     /translation="MNTRVLVSIDNYETRAAILENSVLTEYLFERNEHRRMNGNIYKA
                     RVCSIIPGMQASFVDIGLGRNAFLFVTDVVAPTWSGYRDMLELDGETTELDEEEIDET
                     WHHDVPLPDLSIEDLMKEGQEILVQMEKEPIGDKGARVTANISLPGRFVVLLPNSRHV
                     GVSRKISSLEEKQRLRDIGKQILHEEYGLILRTAAEGISAELLQADMEGLVRQWERIK
                     RSSDESRAPALLYHEMGLAQRILRDCLNHDSAEFIVDSPGLYEQLRDYAHVLRPDLDC
                     LVTLYEGRAPLLRAHGIESEVEKLLERKVWLDCGGHIVIDEAEALTVIDVNTGKYTGD
                     RDLEETVLRANLEAGQEIARQLRLRNIGGIIVIDFIDMKQKENWDTLIAHLESFLQKD
                     RAFTRVLGVTDLGLVEMTRKRVHKSLGRSLCQACPYCRRDGMVLSAQTMAIKALRRLE
                     AITDEQPGKSVTLYVHPAVAGQVHQEFGSSLEAIRAKTGKDVDVVGHEQIHFEEIRLK
                     PVAASSEAVLNLSL"
     gene            2160..2471
                     /gene="rplU"
                     /locus_tag="PKH07_06490"
     CDS             2160..2471
                     /gene="rplU"
                     /locus_tag="PKH07_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HNT34626.1"
                     /translation="MYAIIETGGKQYRVSVGDLIDVEKLANEPGDSIEFGRVLLVNKG
                     EQPEMGTPCIEGAKVIGRLISQGKARKVIIYRYKRRKNYRRKKGHRQPFSRVRIEQIV
                     A"
     gene            2670..>3208
                     /locus_tag="PKH07_06495"
     CDS             2670..>3208
                     /locus_tag="PKH07_06495"
                     /inference="COORDINATES: protein motif:HMM:NF013205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HNT34627.1"
                     /translation="MFVDRVKVKVRAGSGGNGCISFRREKYVPHGGPDGGDGGHGGSI
                     YFLVHPGETTLLNLHSRPSYRASDGGHGLGKCMHGADGQDIILEVPPGTVVHNRADMS
                     LIGELLREGDRLLVARGGRGGRGNTHFKTPERQAPRRATPGVPGEERELLVELKLVAQ
                     GGLVGLPNSGKSTLLRRLTR"
BASE COUNT          729 a          765 c          979 g          725 t
ORIGIN      
        1 tcgagaaaga accgaattgg agatgtctgt tcgagtccgc gatggaagaa cgccaaaggc
       61 acgagacctc tcggcgtgga tttctctgtc gctgaggcag cttggttttc agccatcttc
      121 cgaaagacga ctctgcgacg aaaaagcgct gttgtcgtct gtagatatga ttgagagtgc
      181 agacagcctc tgtgcagagg gcggtctgtt tactctggag aaggtggagg ttttgagtcg
      241 gacacctgaa ggtgatttgg tgtctttgat ggatgttcca gtgccagagg ctggnnnnnn
      301 nnnnaggctg gagacggcgg gtctacaccc gcgggcacgt gggctatgcc cactcccgtg
      361 cggtgcttgc tcacagaaga gacgactccg tcctgaggag gcaagcgcct gcaggtgtgc
      421 gggctatgcc cgctccgacc aacgcctgtt cattggaaga actagacttc ttcgaagtct
      481 ggtttcctgg gagttttatc ttaaaggagc aggtcttcac ccacgggcat tcgggcaaag
      541 tccagtgcga cggacaagag tggaaatgaa taccagggtt ctggtcagca tagacaacta
      601 cgaaacacgg gcggcaattc tcgagaattc cgtgctgacc gaatatcttt ttgaacgaaa
      661 tgagcacagg cggatgaacg gcaatatcta caaagcccgc gtgtgttcaa tcattcctgg
      721 aatgcaggct tcatttgtgg acataggtct gggtcgaaac gctttcctct ttgtgacgga
      781 tgtggttgcc cctacctggt cgggctatcg ggatatgctc gaactcgatg gagaaaccac
      841 ggagctggat gaagaggaga tagatgagac ctggcatcat gatgttcccc ttcccgatct
      901 ttccatagag gatctcatga aggaaggtca ggagatcctg gttcaaatgg agaaggaacc
      961 catcggggac aagggtgccc gagtgacagc aaacatttcg ctgcccggtc ggttcgttgt
     1021 cttgttgccc aactccaggc acgtcggcgt gtcccgaaaa atctcctcgt tggaggagaa
     1081 gcagaggctc cgtgacattg gcaagcagat attgcatgag gaatacggct tgattcttcg
     1141 aaccgccgca gaaggaatct cagcggagtt gcttcaagcg gatatggaag gcctggttcg
     1201 tcaatgggaa cgtatcaaac gcagttcaga tgagagtcga gcgcctgcgc ttttatatca
     1261 cgagatggga ctggcacaac gaatcctcag ggactgcctg aaccacgatt cggctgaatt
     1321 catcgtggat agtcctgggc tctatgagca actgcgtgac tacgcccatg tgcttcgacc
     1381 ggatctggac tgtctggtga ccctctatga aggacgggcg ccactcttga gagcacatgg
     1441 aatcgaaagc gaagtcgaaa aacttctcga gcgcaaggtc tggctggact gtggcggaca
     1501 catcgtcatt gacgaagcgg aagccctgac cgtcatagat gtcaatacag gcaagtatac
     1561 gggcgaccgc gatcttgagg agaccgtgct tcgcgccaac ttggaagcag gccaggagat
     1621 tgcccggcaa ctacgacttc gaaacatcgg tggaatcatt gttattgact tcatagacat
     1681 gaaacagaag gagaattggg acacgctgat tgcgcacctg gagtcgtttc ttcaaaaaga
     1741 tcgggcgttc acccgggtgt tgggggtcac cgatcttgga cttgttgaga tgacccgaaa
     1801 gcgtgttcat aagagcctgg gtcgctcttt gtgtcaggcg tgtccatact gcaggcgcga
     1861 tggcatggtt ttgtccgcac agacgatggc gatcaaagcg ctcaggcgtc tggaagccat
     1921 cacggacgag cagcctggca aatccgttac cctttatgtt cacccggcgg tggcgggaca
     1981 ggtgcatcag gagttcggtt caagtttgga agcaattcga gctaagacgg gcaaggacgt
     2041 ggatgttgtt ggccatgagc agattcactt tgaagagatt cggctcaagc cggtggccgc
     2101 ttcttcggaa gccgttctga acctgtcgct ctgaacagat tttgtttttg gaggagagag
     2161 tgtacgcgat tattgaaaca ggtgggaagc agtatcgggt ttctgttggc gatctcattg
     2221 acgtcgagaa actggccaat gagcctggag acagcatcga attcggtcgg gtgctgctgg
     2281 tcaacaaggg agaacaacct gaaatgggca ctccctgcat cgaaggagcc aaagtcattg
     2341 gccgtctgat cagtcagggc aaggcacgca aggtgatcat ctacaggtac aagcgccgca
     2401 agaactaccg tcgcaaaaag ggtcatcgtc aacctttttc tcgggttcgc atcgagcaga
     2461 tcgtcgcctg actttgcgtc tgccacaaag tttcggaacg gtgaaccgca ttcattgtgt
     2521 ggattgctgt cctacagaag tgtccgcccg gaagacctcg atctccagcc cgcacgttca
     2581 ccgtgcgggt tgttgcttat aggtgtgttt gcccgcagga cgcgtggtct gtacactgac
     2641 tgtgcgaatt gctgctgagg agttgtctgt tgttcgttga ccgagtcaaa gtaaaggttc
     2701 gtgctggatc gggtggcaac ggatgcatca gtttccgacg cgagaaatat gtgccccacg
     2761 gaggcccgga tggtggtgat ggggggcatg ggggaagtat ttacttcctg gttcaccccg
     2821 gcgagaccac acttctgaat ctccacagcc gacccagtta cagggcatct gacggtggtc
     2881 atggtctggg caagtgcatg catggagccg acggtcagga catcattctt gaggttcccc
     2941 ccggaaccgt ggtgcacaat cgagccgaca tgagtctcat cggggaattg ctccgagaag
     3001 gggatagact tctggtggcg cgtgggggaa ggggaggcag gggaaacaca catttcaaga
     3061 cgcctgagcg acaagctcct cgccgtgcca cgccgggcgt tcccggtgaa gagcgcgagc
     3121 ttctggttga gttgaaactg gtggctcaag gcggtttggt gggtttgccc aattcgggta
     3181 agtcaacact ccttcggcgt ttgactcg
//
LOCUS       DAOQUC010000305         3187 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_35097_length_3187_cov_2.961367, whole genome shotgun sequence.
ACCESSION   DAOQUC010000305 DAOQUC010000000
VERSION     DAOQUC010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3187
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35097_length_3187_cov_2.961367"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            304..1407
                     /locus_tag="PKH07_06500"
     CDS             304..1407
                     /locus_tag="PKH07_06500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34628.1"
                     /translation="MLRTRAGLGSGVELILAGRLYVNVPVFERFAAESPLTLFCRDGE
                     YFIHVDPRRSVPVRLAPRPWFYDEKTSSGVEMHRIGVMQGTTLGIYPSKVCEFWEMEP
                     RVNCRFCSTGLNVGVSEEGGKTVRDVVEVCQAARKLDKITFVHFNTGYYSEDALDIVE
                     PYVVAVKKETGLLIGVQCPPSRDLKKYDHLKAVGVDHLSFCYEFHNPDFFARHCPGKA
                     IHLGQKAFFEAIEHTSHLFGKGRVSGEIIAGLEPIEDTLRAIDYITSVGAFPTICIFR
                     PLLGTDAEQETSPSAEDMRRVFRHLYERLRDHSIMTGIAPGVRTSLVILPFEASYFRK
                     GWRLSDVLYAAKTCLARLIYGLYFRARLLRFPN"
     gene            1433..2308
                     /locus_tag="PKH07_06505"
     CDS             1433..2308
                     /locus_tag="PKH07_06505"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNT34629.1"
                     /translation="MKSQIATTRQAIEIALRQYLPSANGRDNQISKAIEYIVSAPGKR
                     LRPVLMLEIARCLGFASEQIMPSACAIEYIHTSSLLLDDLPCMDDAKVRRGQPTVHLA
                     FNEYTAILASIALLMEAFALIARNAKQLNVPQAGIADLVSDTAQTIGLKGMIVGQYLD
                     LSLEDSHLEQQQVDYIHDHKTTVLFEASVNIACTLCGVERQIRDALVEYGRTIGRAFQ
                     IADDILDASDEVQPRSKDKHKDAGKTTYVNLLGIEGSKKRLHVLVETAVEAVQVVPSN
                     ENLQVLARYIEEQVS"
     gene            2305..>3187
                     /locus_tag="PKH07_06510"
     CDS             2305..>3187
                     /locus_tag="PKH07_06510"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HNT34630.1"
                     /translation="MKGISAEDSGGLKVFLELFRPFTLLPPATGIFCGALCAIGAWSR
                     TDADLAALEILQRRWFLILVAATAASILNAASNVLNQYTDKDIDSINKPSRPLPSGRV
                     TERSILILTGVLFSVGLLTAWALSLCGLGDFFVLALLGTVSTVLYSVKPFRLKRFLYF
                     SNLAIAVPRGYLLIVAGWSCATSVSVFEQREPWYLGMVLFLFLLGAASTKDFSDMRGD
                     QLGGCVTLPIKFGARKAAWMIAPFLTLPWLLLPYDVLRRMNHHHIGVLSADRVLLLLL
                     GLGLAGYGAYVSHLIVRA"
BASE COUNT          691 a          827 c          860 g          809 t
ORIGIN      
        1 gtgactgttg aaacggaaga aacctatacc tggacgatga cgatctacgc tccggaaaaa
       61 cctggtgact atctcaccga ctggcagatg cacaaggaag gtctcggtgc gtttggtgat
      121 atcattcgcc atgagattac agttctgccc aatacgtcgg gtcagacgga gtgaacgctt
      181 gagactcaat cccggactgt ggtatatgac ttgcccatga accaaggcga actcaaactc
      241 cttcttctct gccacggtgt cagattggac tcttcctgtg acctggaacg tgacgggaga
      301 cccatgctgc gtacgcgtgc gggtctggga agcggggttg aactcatcct ggcaggccgt
      361 ctctatgtca acgtccctgt ttttgaaaga tttgcggccg agtcccccct tacgctcttc
      421 tgcagagacg gtgaatactt catccatgtg gatccccgcc gctccgtgcc cgttcgactt
      481 gctcccagac cttggttcta tgatgagaag acttcatccg gagtcgagat gcatcgaatt
      541 ggtgtgatgc agggcaccac tcttggcatc tatcccagca aggtctgcga gttctgggag
      601 atggagccaa gggtgaactg ccgattctgc tccaccggac tcaatgtggg tgtgagcgaa
      661 gaggggggca agacagtcag agacgttgtg gaagtctgcc aagcggcaag aaagttggac
      721 aagatcactt ttgttcattt caacacaggg tactattcgg aagatgcact cgacattgtg
      781 gaaccttatg ttgtggcggt caagaaggaa accggtctgc tcattggggt gcagtgtccg
      841 ccgtctcgtg acttgaagaa atatgatcac ctgaaggctg taggagtgga tcatctgtca
      901 ttttgctatg agtttcacaa tcccgacttc tttgcacggc attgtcctgg caaagcaata
      961 catctgggac aaaaagcctt ttttgaggcg attgagcaca cctcgcacct ctttggcaaa
     1021 ggtcgtgtgt cgggtgagat catcgcagga ctggagccaa ttgaggatac gcttcgagcc
     1081 attgattaca tcaccagtgt gggcgctttt cccacgatct gcatcttccg tcccctgctt
     1141 ggaaccgacg cagaacagga gacctcccca tcggcggaag acatgagacg tgtcttccgg
     1201 cacctgtatg agcgtcttcg agaccactcg atcatgacgg gaatcgctcc tggtgttcgc
     1261 accagtctgg tgattcttcc ctttgaggcc agttactttc gcaagggatg gaggttgtcg
     1321 gatgttctct acgcggcgaa gacctgtctg gctcggctga tttatggttt gtactttcga
     1381 gcaagactgc taagattccc aaattgacat ccaagacagg atgaaagaca gaatgaagtc
     1441 tcagattgca acaacgcgac aggccatcga aattgctttg cgtcagtacc ttccttccgc
     1501 caacggtcga gacaatcaga tatctaaggc tatcgagtac attgtatcgg cacctggaaa
     1561 gaggctgcgt ccggtgctta tgcttgagat cgcccgctgt ctcggttttg cctctgagca
     1621 gatcatgccg tcagcgtgtg ccattgagta catccacaca tcgtcactgc ttctggatga
     1681 tctcccctgc atggacgatg ccaaggttcg ccgaggacaa ccgaccgtgc atctggcttt
     1741 caacgagtac actgccatcc tggcatccat cgctctgctg atggaagcct ttgccctgat
     1801 cgcccgaaat gcgaagcagc tcaatgttcc gcaggcgggg attgcagatc tggtgagcga
     1861 cactgcccaa accatcggtt tgaaaggcat gatcgtgggt cagtacctgg atctttcgct
     1921 tgaggattcc cacttggaac agcagcaggt ggactacatc cacgatcaca agaccactgt
     1981 tctttttgag gcctcggtga acattgcctg caccctttgc ggcgttgaga ggcagatacg
     2041 tgatgccctt gtcgagtatg gcagaaccat tgggagagcc tttcagatcg ccgatgacat
     2101 ccttgacgcc agcgacgaag tacagccccg cagcaaggac aagcacaaag acgcaggcaa
     2161 gacaacctat gtgaaccttc tcggaattga aggctcaaag aagagattgc acgtgcttgt
     2221 cgagacggct gttgaagccg tacaagtcgt tccttcgaac gaaaatctcc aagtgctcgc
     2281 cagatatatc gaggaacagg tctcttgaaa ggcatcagcg cagaggatag tggcggcctg
     2341 aaagtctttc tggaactgtt tcgtcctttc accctgcttc cgcccgcaac ggggatattc
     2401 tgtggtgctc tgtgcgcgat aggagcgtgg agtcgcacgg acgccgatct tgccgcgctt
     2461 gaaatcctgc agaggcgttg gtttctcata cttgtggccg ccactgctgc cagtattctg
     2521 aacgctgcct caaatgtcct gaatcagtac acggacaagg acattgacag catcaacaaa
     2581 ccttcaagac cactgccttc cggtcgtgtg acggagcgga gcattctgat cctgacaggt
     2641 gttcttttct ctgtgggact tctgaccgcc tgggcgcttt ccttgtgtgg tttgggagac
     2701 ttctttgttt tggcattgct gggaactgtt tccacggtgc tgtactcagt caaaccattt
     2761 cggcttaaga gatttctcta cttttcaaat cttgccatcg cagttcccag aggatacttg
     2821 ctgatcgttg ctggatggag ttgcgccact tctgtcagcg ttttcgaaca aagggagcct
     2881 tggtatttgg gcatggttct ctttcttttt ctgttgggtg ccgcatccac gaaggacttc
     2941 tcagacatgc ggggtgacca acttggtggc tgtgtcacat tgccgatcaa attcggtgcc
     3001 cgcaaagccg cttggatgat cgcgcccttc ctcacgcttc catggttgct attgccctat
     3061 gacgttctcc gaaggatgaa ccaccatcat atcggtgtgc tctcggcgga tcgggttctg
     3121 ctgcttcttc tcggtcttgg cctggcggga tatggtgcct atgtaagcca tctgattgtg
     3181 cgcgcac
//
LOCUS       DAOQUC010000306         3185 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_35128_length_3185_cov_2.583387, whole genome shotgun sequence.
ACCESSION   DAOQUC010000306 DAOQUC010000000
VERSION     DAOQUC010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3185
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35128_length_3185_cov_2.583387"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1004)
                     /locus_tag="PKH07_06515"
     CDS             complement(<1..1004)
                     /locus_tag="PKH07_06515"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNT34631.1"
                     /translation="MNLVVVWHAGAAFPERLEALANQPDVDLTLIVPQVWTKGLVQPA
                     IASKMQQTSFPVIALHAACQIKEVFHYYPALSETLHKLKPALLYLIEEHYSFVTFQAL
                     RWRERHSPKTAFAFRTWQNIAKNYPVPFRWMERHVLKRADGAISANQEGAAILQRKGL
                     AGKLAIIPDGVDTRRFNIEVGLEKRVEILKRFQVQRESVPLIGFIGRLVPEKGLETLF
                     RAFSEVSSEARLVMIGAGRMQKELEGLSRVLGIQDSVLFADAVSRNDMPHWLAALDVL
                     VLPSLSRPNWKEQFGRILVEAMACGVPVIGADSGEIPQVIGDGGVVFAQGDVLALSEW
                     L"
     gene            complement(1001..2521)
                     /gene="murJ"
                     /locus_tag="PKH07_06520"
     CDS             complement(1001..2521)
                     /gene="murJ"
                     /locus_tag="PKH07_06520"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HNT34632.1"
                     /translation="MTEISGILPWKRFFKWGAGIAGLAVLSRCSGLIREMVVAHCVGI
                     GESVDVYYLASAVPLFVAWMGDAATTQTVLPLFTRRIEADSLESAWRLACGVLWIALL
                     IVAVPVVAATVAPLALWRAISASDGASNAALARMIILLSLPVMLVQTVGGWQTGILQS
                     RKRFFLPAAIPMAYNLALVFVLLVGARHWGVLALAVGLLAAEAMRTLLQWPTLVRMGI
                     RMHHVFRPDWTSARRSLTHLIPIASVVGLQQIVFITDRLMAKGLPEGSVSALAYGHRL
                     LILPYSMLVAAVATPLFSMFSECAAQDDIPRLKEWVVGGLRLMIRLGLPITLMLVVLR
                     VPLVRMLFQHGAFDEHATQLASSAVLGYGMSLVFFAVLPVLSQYFYAVGEVRTPLLAS
                     GVCAVLNIGLNTLFRDLWSHAGIAVASAISFAVCAGLHLPALQKRLGSLAKEGHLMLR
                     EFLLSGLAMGFCMAVCLVLAQRMAFSRPIVADLVTTTIVGGVGVLAGLVVWRLSKR"
     gene            complement(2570..>3185)
                     /locus_tag="PKH07_06525"
     CDS             complement(2570..>3185)
                     /locus_tag="PKH07_06525"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HNT34633.1"
                     /translation="VILNRKSLNKLIEPLPLESIRGGFIARCIAFIIDWVVAIPKFAF
                     VLKHYKLGTETDFQYLLMIETVLAFFYFLLFNYGVGMTLGKLFAGLRVVDSMGRMISA
                     GQAFGRAIISFLSLSLVPAVVHAICFFVSPERKGLHDWLLFTLVVKDEPWKKMAKEAV
                     AFGGRLPKKPSPKTAPKEPSESQSWETQSESQEDKIDDTTASYY"
BASE COUNT          834 a          913 c          829 g          609 t
ORIGIN      
        1 agccactcgc tgagcgccag cacatcccct tgggcgaaaa ccaccccccc gtctccgatc
       61 acttgcggaa tctcgcccga gtcggctccg atcaccggca caccacaggc catggcctcc
      121 accaggatgc gcccgaattg ctctttccag ttcggtcgac ttaacgatgg cagcaccagc
      181 acatcgaggg ctgccagcca atgaggcata tcgttgcggc tgactgcgtc cgcaaagaga
      241 acggaatcct ggattcctaa aactcttgag agaccttcga gttccttctg catgcgacca
      301 gcaccgatca tcacaagtcg ggcttcggaa gaaacttctg agaacgcgcg aaacaacgtc
      361 tcaagcccct tctccggcac aagccttccg atgaagccaa tcaagggaac agactccctc
      421 tgcacctgga aacgtttgag aatctcaacc cgtttctcca gacccacttc gatgttgaat
      481 cgtcttgtat ccaccccatc tgggatgatt gccagttttc cagccaaccc tttgcgctgg
      541 aggattgctg cgccttcctg atttgccgaa atggctccgt cggcacgctt taagacatgg
      601 cgctccatcc atcggaacgg cacgggatag ttcttcgcga tattctgcca ggtgcggaag
      661 gcaaatgccg ttttgggtga atgtctctcg cgccatctga gcgcctggaa agtgacaaat
      721 gagtaatgct cttcgatcag atagagcaaa gcgggcttca gtttgtgcag ggtctcgctc
      781 aatgcagggt agtaatgaaa gacctccttg atctggcagg ccgcatgaag cgcgatgaca
      841 ggaaaggaag tctgctgcat cttggaagca atcgcaggtt gaaccaagcc ctttgtccac
      901 acctgaggaa cgatcagagt gaggtcaacg tccggctgat tcgccaaggc ttcgagtcgc
      961 tcgggaaatg ctgctcctgc gtgccaaacc acaacgagat tcatcgcttt gaaagcctcc
     1021 agacgacaag accagccaga acacccaccc cacccacaat tgtcgtcgtg acgagatcag
     1081 ccacgatcgg tcgtgagaag gccattctct gagcgagcac aaggcataca gccatgcaaa
     1141 agcccatcgc cagccctgag agaaggaact cccgcagcat gagatgtcct tcctttgcca
     1201 aagagcccag tctcttctga agcgcgggaa gatgcaagcc cgcacacacc gcgaaagaga
     1261 tggcactcgc gacagcgatt ccagcgtgac tccacaagtc tcgaaacaga gtgttgaggc
     1321 cgatgttcag cacagcacac acgccgctgg cgagaagagg tgttctgacc tctccgacgg
     1381 catagaaata ctgtgaaagc acaggcaaga cagcaaagaa gaccagactc atgccgtatc
     1441 caagtacggc ggaactggca agttgtgtgg cgtgttcgtc aaacgccccg tgttggaaaa
     1501 gcattcgaac caacggcacc cgaagcacca ccaacatcaa ggtgatgggg agtcccaaac
     1561 ggatcataag cctcagtcca ccgacgaccc attctttgag cctcgggatg tcatcctgcg
     1621 cggcgcactc cgagaacatg gaaaacaggg gagtggcaac ggctgcaacc agcatggagt
     1681 aaggaagaat cagcaaccga tgtccgtagg ccaatgccga cacagagcct tcgggaagtc
     1741 cctttgccat cagtcggtct gtgatgaaaa caatctgttg gagcccaacc accgaagcta
     1801 ttggaatcaa atgcgtcaga gatcttctcg cggaagtcca atcgggtctg aagacgtgat
     1861 gcattctgat gcccattcga acgagcgtag gccactgcaa gagtgtccgc attgcctccg
     1921 ctgccagaag accgacggcg agagcaagca caccccaatg tcgagcaccc accagcagaa
     1981 caaacaccag agccaggttg taagccatgg gaatggccgc gggaagaaag aaacgttttc
     2041 gggactgaag aattccggtc tgccagcctc caacggtctg aaccagcatg accggcagag
     2101 agagcaggat gatcattcgc gccagggcag cgttggatgc cccatccgaa gcggagatcg
     2161 ctctccagag agccaacggt gcaacggttg ctgcaacaac aggcacggcc acgatcaaca
     2221 gggcgatcca aagaactccg caggcaagac gccaggcgct ttccaaggag tctgcttcga
     2281 tgcgacgagt gaagagtgga agcacggtct gagtggtagc ggcatcgccc atccaggcaa
     2341 caaacaaggg aactgcactt gcaagataat acacatccac cgattcgccg attccaacgc
     2401 agtgggcgac caccatttcg cgaataagtc cggagcatcg gctcaacacg gcaaggccag
     2461 caatgccagc gccccatttg aagaaacgct tccacggtaa gattccagag atttctgtca
     2521 agttatcaga atgctccctg atgcacggca gatgatgaag gccgatcgat cagtagtacg
     2581 acgctgttgt gtcatctatc ttgtcttcct gtgactccga ttgtgtctcc caactctggg
     2641 attcgctcgg ctcttttggc gctgtcttcg gggatggttt ctttggcaag cgcccaccaa
     2701 atgcgactgc ttctttcgcc attttcttcc agggttcatc tttcaccacc aaggtgaaaa
     2761 gaagccagtc gtgcagtccc ttgcgctcgg gggaaacgaa gaagcatatc gcgtgaacta
     2821 ctgcaggaac aagtgacagc gagaggaacg agatgatggc tcggccaaaa gcctgtcccg
     2881 cagagatcat ccttcccatg gagtccacaa cacgcaaacc ggcaaaaagt ttcccgagcg
     2941 tcatcccaac accatagtta aacaacagga aatagaagaa ggcgagcacc gtttcaatca
     3001 tcagcagata ctgaaagtcc gtctcggttc caagtttata gtgcttcaga acaaaggcga
     3061 actttggaat ggcaacaacc cagtcaatga tgaaagcaat gcaacgcgcg atgaatcccc
     3121 cccgaatgct ttcaagaggc aggggttcga tgagtttgtt gagactcttc cggttcagga
     3181 tcacc
//
LOCUS       DAOQUC010000307         3177 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_35279_length_3177_cov_2.536835, whole genome shotgun sequence.
ACCESSION   DAOQUC010000307 DAOQUC010000000
VERSION     DAOQUC010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3177
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35279_length_3177_cov_2.536835"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="PKH07_06530"
     CDS             complement(<1..334)
                     /locus_tag="PKH07_06530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34634.1"
                     /translation="MHRISVVVLWLILQCCLCQAQVIIDHRCTDASQIPEAWILQAKE
                     DLHIAYQHTSHGSQLTTGIEILNGRNPGGLFAFEEGGGEGLLDYRDYAMQSYAEPPTD
                     YMDLGQPTL"
     gene            complement(488..1537)
                     /locus_tag="PKH07_06535"
     CDS             complement(488..1537)
                     /locus_tag="PKH07_06535"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNT34635.1"
                     /translation="MSQPIFRIQNRSLIRFFQAVAVAAFLLTLAFQGYTFVMKEHAER
                     PLPPLFGNFNDNQDILERIPPKEEFSFAVAGDTRSFGTFERICEELRKTDIDFAVLLG
                     DCTYTGTEEQHQYLRAEWVREYALPCPVFYLVGNHDINPKTFPLNRFEEVYGPSIFSF
                     EYQGSLFIMLRILHEKRGSNEESLAFLRSFRNKPLDQYKYIFAFMHMPPHISPFFHSK
                     GYPDEKELVSLFEELGVDYVFSGDFHGYARVKLRETTFIISGGGGGHLEKQPSGQFTH
                     ALMMRVTPDSVEERIVHVPESKGFVDRMERFAIVRFWPKMSRYPSVTVTLNLAVLLAL
                     ILFLRFTSKCLKAAA"
     gene            1716..3011
                     /locus_tag="PKH07_06540"
     CDS             1716..3011
                     /locus_tag="PKH07_06540"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HNT34636.1"
                     /translation="MERSKIESRLKLGMRIILPALLLILAYWQRKVLGGVLKPVLVGL
                     LLAYIFDPFVTRLERGRFRMPRQCGILLLGLTMLVFIVLVGWLLIPGLVRELADLVAR
                     VPDYAQLAGDFLYDRFGKTWEALPETTRDWLRGFFSRDRLLQTWQDRIIPYLEQTGYD
                     ENAQAVARGIRDASSAIIGGVVFTLGLLLGGVTGAFNLVFSLVLAVIVSFYLLKDFPN
                     IKQKVVERVAEEHRDHFLSVVNELDTLVSGFFRGQLLVCFCIAVMTGIGMVGLGVNKP
                     LLVAFCAGAFNVVPYLGPVMGAMPAIVLTVAEYYPMGFMTDVMPRVIGIVIWFAFVQT
                     MDGLVISPRIMSRTVRLHPLIILFALLVGGQLFGLVGIMLAVPTVAVIKILCRQVYII
                     LYKRPTGTSPQVPVAADSLSGGADASEAQGNEGQEQTDQ"
     gene            2983..>3177
                     /locus_tag="PKH07_06545"
     CDS             2983..>3177
                     /locus_tag="PKH07_06545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34637.1"
                     /translation="MKDKNKLTNEEIVSSIREKLSQKNRRIAELEQTIAALRESLSSN
                     QKELEGRDRLFQMLSDLLGEE"
BASE COUNT          762 a          758 c          848 g          809 t
ORIGIN      
        1 ccagagttgg ctgccccaga tccatgtaat ccgtaggagg ttctgcgtaa gattgcatag
       61 cgtaatctcg gtagtccaga agcccttccc ctccaccttc ctcgaatgca aagagccctc
      121 ccggattccg tccgttgaga atctcgattc cggttgttaa ttgactcccg tgcgaagtgt
      181 gttgataggc gatatgcaga tcttcctttg cctggagtat ccaagcctcg ggaatctgcg
      241 acgcgtccgt gcatctgtga tcaatgataa cctgtgcttg gcacaggcaa cactggagaa
      301 tgagccagag cacaaccaca ctaatccgat gcaaaaaacc tgttccgtct gttgatgaag
      361 gtatcatttg cgtccctctc ggtgtgagtg aaagcctcaa atatcggtgc cggacaaatc
      421 tttggccact cagcctcatg agatagtcca ataagagttt agcagaaagc tcgaactgtg
      481 caaccagcta tgcggctgcc ttgagacact tggatgtaaa gcgaagaaac aagatcaggg
      541 cgagcagaac agcaagattc aaagtcacag tcacagatgg atatctgctc attttaggcc
      601 agaagcgaac aatggcaaat cgttccatgc gatccacgaa acctttagac tcgggaacat
      661 ggacgatcct ctcttcaaca gagtcaggcg tcactctcat catcagagca tgagtgaact
      721 gaccagaagg ttgcttttcg agatgtccgc cacctcctcc agagatgatg aaggttgttt
      781 ccctcagttt cactcttgcg tatccgtgga aatcgcctga gaagacataa tcaacaccca
      841 gctcttcgaa gagactgacc agttccttct catcaggata cccttttgag tgaaagaacg
      901 gtgaaatatg gggcggcatg tgcatgaagg cgaaaatgta cttgtactga tcaagtggtt
      961 tgttccggaa tgaacgaagg aaggctagac tctcttcatt cgaacccctc ttttcgtgaa
     1021 gtatacggag catgatgaag agagagcctt ggtattcgaa actgaagatg ctcggcccat
     1081 agacctcctc aaaccgattg aggggaaatg tcttgggatt gatgtcgtgg tttcccacca
     1141 ggtagaagac gggacagggg agggcatact ctctcaccca ctctgctctc agatactgat
     1201 gctgctcttc agttccagtg taggtgcagt ctcccagcaa gacggcaaag tcaatatccg
     1261 tcttgcgcaa ctcttcacaa atcctctcga aagtaccgaa actcctcgta tcaccggcaa
     1321 ctgcgaagga gaactcctct ttgggcggaa tgcgctctaa aatgtcctgg ttgtcattga
     1381 aattgccaaa cagaggagga agaggtcttt ctgcgtgctc cttcatgacg aacgtgtaac
     1441 cttgaaatgc aagagtgagc agaaatgcgg ccactgccac agcctggaaa aaccgaatca
     1501 gactcctgtt ctgaatccgg aagataggtt gggacatagg gcaccctctt ttcattgctg
     1561 tctgatggac gaggcaggac agatctgatg ccaacctgag aacgcctcga agtacaccct
     1621 gagaatgata tctgaatcgt tgacaaagga caattgcatt tgccacatta gatatgagat
     1681 actctgttgg caaagcaaat gtaaagagga atcaagtgga aaggtctaag attgaatcca
     1741 ggctaaaact gggcatgagg atcattctgc cggcgttgtt gctgatcctc gcctactggc
     1801 aacgaaaggt tttaggagga gtcctaaaac ccgtcttggt cggactactg ctcgcgtata
     1861 tctttgatcc gtttgtcacg cgccttgaaa gaggacgttt caggatgcca agacaatgtg
     1921 gcatattgct gcttggtctc acaatgcttg tctttatcgt gcttgttgga tggctcctca
     1981 ttcctggtct ggtgcgagaa ctagcggatc tggttgcgcg agttcctgac tacgcgcagt
     2041 tggcggggga ctttctctac gacaggttcg ggaaaacttg ggaggcactg cctgagacaa
     2101 ctcgcgactg gcttcgagga ttcttctctc gtgacagatt gctgcagacc tggcaagaca
     2161 ggataatccc ttatcttgag cagacaggct acgatgagaa tgctcaggct gttgcgcgtg
     2221 ggatacgcga tgcctcctct gctatcattg gtggtgttgt tttcaccctc ggtttgctcc
     2281 tggggggtgt gacgggagcc ttcaatctag tgttcagctt ggttcttgcg gtcatcgttt
     2341 ccttctacct tctgaaggat tttccgaaca tcaaacagaa ggttgttgaa cgtgttgctg
     2401 aagagcatcg agaccacttc ctgagcgttg tcaatgagct ggatacgctg gtgagtggat
     2461 tcttcagagg tcagctgctt gtctgcttct gcatcgcagt catgactggg atagggatgg
     2521 tgggattggg tgtgaacaag ccgctgctgg ttgcattctg tgcgggagcg ttcaatgttg
     2581 ttccctatct gggacctgtc atgggagcca tgccggctat tgttctcacc gttgcggagt
     2641 attacccgat gggtttcatg actgacgtga tgccgcgcgt gattgggatc gtgatctggt
     2701 tcgcctttgt gcaaaccatg gacggcttgg taatttcgcc caggatcatg agccgtacgg
     2761 ttcggcttca cccattgata atactcttcg cgctgttggt tggggggcag ttgtttgggt
     2821 tggtgggcat catgcttgcc gtaccaaccg ttgcggtcat caaaattctt tgccggcagg
     2881 tttacatcat tctctacaag cgaccgactg gcacttctcc tcaggtgccc gtcgctgcag
     2941 attccctgtc aggaggtgcg gatgcttcag aggcgcaagg aaatgaagga caagaacaaa
     3001 ctgaccaatg aagaaattgt ctccagtatt cgagagaagc tctcccagaa gaaccgtcga
     3061 atcgctgagt tggagcagac gattgccgct ttgcgagagt ctctctcctc aaaccagaag
     3121 gagttggagg ggagagacag actcttccag atgctttccg atctgctggg tgaggag
//
LOCUS       DAOQUC010000308         3170 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_35377_length_3170_cov_2.887319, whole genome shotgun sequence.
ACCESSION   DAOQUC010000308 DAOQUC010000000
VERSION     DAOQUC010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3170
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35377_length_3170_cov_2.887319"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..416)
                     /locus_tag="PKH07_06550"
     CDS             complement(<1..416)
                     /locus_tag="PKH07_06550"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010936446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HNT34638.1"
                     /translation="MRSWHNIRWSTLKMDPTRMKDWQIAEAASANMKPIAKLADELEL
                     YNSEIIPMGHHIGKVDYARVLNRVKENPSAKYIDVTAITPTPLGEGKTTTTIGLIQGL
                     GVLGKKVTGAIRQPSGGPTFNIKGSAAGGGLSQCVPL"
     gene            complement(468..>3170)
                     /locus_tag="PKH07_06555"
     CDS             complement(468..>3170)
                     /locus_tag="PKH07_06555"
                     /inference="COORDINATES: protein motif:HMM:NF021126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HNT34639.1"
                     /translation="YSGMPFLADVQSAVLYPLNLALTFFVDEGALSFAALQNQIIFHY
                     FLAGAFMFALLRHFRLGRFSCLIGSVSFMFCGFLVAHLGHVGMSNVAVWIPLVFLFFH
                     KSLTERHWLSAVWAGFFYGICIMGGHSQPALYMLATMSLYVLWSVLASIMEQRPNRLS
                     HSFSQIGLFALMMLVTLGVAAAQIAPMAELALHSIRAEEGFDFATSYSLPPRQLITLL
                     IPKFFGMTVPGERDYWGEDNFTEMCVYVGILPIVLAAIALLYRKDRMTRFFGLLALFS
                     LLMALGKHTPLYRLLYALPTPLRLVRVPARFGYVLGFSTAILAGFGAETFLSELNESA
                     RISLRRILVATVVLMLMGSLLLAALYGSWIANSQAEDSWKYATAADQTLLFLLILFSC
                     WMVLFMRTWKNMSPLASGISLLVVLVVDLLAFGAEYNLAKGTPEQVQYERNAAVDYLK
                     AEIGTGRFEQTVGIPYNTATRHGIFDVEGWNPFSLNEYDVFRKGVKSPQKRGQPEDVE
                     RLRYVLGVNFVVGKHLQLARERRTVNAIEFDASTFESVGPAGEPSLLLDNLPVVLTDR
                     IGLVSDLGYAVETPQGTLVGELEVETFSAKRWRFPIRAGIESSEWACDYRDNLPYMKH
                     LPARVAWEHVRGDIPAKTFIAEFDLPELEYIRSLHIVPQDSKAVINVHSFSLIDSQSG
                     QSVPIGAGGIRFDQTLNDIIILRTRSALPRALLVHEVEWAQSFEDSIEKVREQAYDLR
                     EKVILEGSSDQQEDVPLGAGVGELVQVSVYRPNEVVIRVEAHAAGVLVLNDVLYPGWR
                     AYLDGKRVRLFRANGIMRAVAVPPGRHWIRMAYRPRLVKQAGRASLLTLLIVALLAFL
                     PIFGWFQRTFLRCRPADSVSQVHSDSRCRRGIAVRFTTPPAIQY"
BASE COUNT          853 a          822 c          819 g          676 t
ORIGIN      
        1 agaggaacgc actggctcag acctccgccc gccgcagacc ccttgatgtt aaatgtaggt
       61 ccgccactgg gttggcgaat tgcgccggtc acttttttgc cgagaacacc aagaccttga
      121 atcaagccga tcgtggtcgt cgttttgccc tcaccgagag gagtcggggt gatggcagtg
      181 acgtcaatgt acttggcaga tgggttctct ttgacacggt tgagaacgcg cgcgtaatca
      241 actttgccga tgtgatgacc cattgggatg atttcactgt tgtacaactc cagttcatcg
      301 gcaagtttgg caatgggttt catgttcgca gacgcagcct cggcgatctg ccagtccttc
      361 attctggttg gatccatttt cagagtactc catctaatgt tgtgccatga acgcatgaca
      421 aagggtcaac tctattcaca acaaggtatt ggaatgcttc cggcaagtca atactggatc
      481 gccggtggtg tggtaaagcg aaccgcaatg ccccttctgc atcgggaatc ggagtgcacc
      541 tgactcaccg agtcggcggg tctgcatctt aggaatgttc gttgaaacca tccgaaaatc
      601 ggaagaaaag ccaagagcgc aacgataagc aatgtcagaa ggcttgctcg tcccgcctgt
      661 ttgacaagcc ttggacgata ggccattcga atccaatgcc ttccgggtgg taccgcaacg
      721 gcgcgcatga tcccattggc tcggaaaagc cgaaccctct ttccatcaag atatgcacgc
      781 caacctgggt aaagtacatc gtttagaacc aggacacccg cagcgtgtgc ctcaactctt
      841 atcacgactt cgttgggacg atacacgctt acctgtacaa gttctccgac tccagctccc
      901 aggggaacat cctcctgctg gtctgaagag ccttcaagaa taaccttctc acgcagatca
      961 taggcttgct ctcggacttt ctctatagag tcctcaaagg attgagccca ctccacctca
     1021 tgaacaagaa gagctcgggg aagagccgat cgggttctga gtatgataat gtcgttcagt
     1081 gtctgatcaa accggattcc gcccgcaccg atgggaacgc tctgaccgct ttgtgagtcg
     1141 atcaatgaga aactgtggac attgataacc gctttggaat cctgaggcac aatatgcaag
     1201 gagcggatgt actccaactc gggaagatca aactcggcga taaacgtctt tgcaggaatg
     1261 tctccacgaa cgtgttccca ggcaactctg gctggcagat gcttcatata gggaagattg
     1321 tcacggtaat cacaagccca ctccgaactc tctatgcctg cacgtattgg aaaacgccat
     1381 ctctttgccg agaaggtctc aacttcgagt tccccgacaa gtgttccttg aggcgtttca
     1441 acagcgtagc ccaaatcact gacgaggccg attcggtcag tgagaacgac gggaagattg
     1501 tccagaagca gagagggttc tccagcaggc cctacagatt cgaaagtgga tgcatcaaac
     1561 tcgatcgcgt tgactgttcg ccgttctctg gccaactgaa gatgtttgcc aacaacgaag
     1621 ttgacaccca gtacgtatct gaggcgctcc acatcctcag gctggcctcg cttttggggg
     1681 gactttacac ccttcctgaa gacatcatac tcattcagac tgaaaggatt ccatccttcg
     1741 acatcgaaga ttccatggcg cgtggctgta ttgtatggaa tccccactgt ttgctcgaaa
     1801 cgtcctgttc ctatctcggc cttcagataa tcaactgctg cattgcgttc gtactgtacc
     1861 tgttcgggtg tcccctttgc gagattgtac tctgctccaa aggcaagcaa atccacaacc
     1921 agcacgacga gcaatgaaat gcctgaggcc agaggactca tgttcttcca ggtgcgcatg
     1981 aacaaaacca tccaacagga gaaaagaatc agcagaaaga ggagcgtctg gtctgctgcc
     2041 gtcgcatatt tccaggagtc ctccgcttgg ctattggcga tccaagagcc atacagtgca
     2101 gcaagcagga gtgatcccat aagcatcaga acgacagtgg ccaccagaat gcgacgaagc
     2161 gaaattctgg ctgactcatt aagttccgaa agaaacgtct cagcgccgaa tccagccaga
     2221 atggcggtac tgaatcccag aacgtatcca aatcgggcag ggacacgcac caagcgaagc
     2281 ggagttggta aagcgtaaag cagacgatag agaggagtat gttttccaag cgccatcagc
     2341 aaggaaaaca gtgccagaag tccgaagaaa cgagtcatcc tgtctttccg atagagcagg
     2401 gcaattgcgg ccaagactat ggggagaatt ccgacataga cacacatctc cgtgaaattg
     2461 tcttcacccc aatagtctct ctcacctgga accgtcatcc cgaaaaactt agggatcagc
     2521 agggtgatta actgacgcgg gggaagggaa taagacgttg cgaaatcgaa tccctcttct
     2581 gcgcggattg aatggagagc cagctcagcc atgggtgcaa tctgggctgc ggcaacgcca
     2641 agagtgacga gcatcatcag ggcgaacagg ccgatctgcg aaaatgaatg agaaaggcga
     2701 ttggggcgtt gctccataat ggatgcaaga acagaccaga gcacatatag actcatggtt
     2761 gccagcatat agagagcggg ttggctgtgc cctcccatga tgcagattcc gtagaagaaa
     2821 cctgcccaga cggcgctgag ccaatgcctt tcggttaggc tcttgtgaaa gaaaaggaag
     2881 accaaaggga tccagaccgc cacattcgac atgccgacat gacccaggtg tgccaccagg
     2941 aatccgcaga acatgaaaga gacgcttccg ataaggcagc tgaatcgtcc caaacgaaaa
     3001 tggcgaagca gcgcaaacat gaaagcgccg gcaaggaaat agtgaaagat gatctgattc
     3061 tgcaatgccg caaaagacaa ggctccctca tctacaaaaa aggttaacgc gaggttgagg
     3121 ggatagagca ctgctgactg cacatcggcg agaaaaggca tcccagaata
//
LOCUS       DAOQUC010000309         3164 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_35458_length_3164_cov_4.273078, whole genome shotgun sequence.
ACCESSION   DAOQUC010000309 DAOQUC010000000
VERSION     DAOQUC010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3164)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3164)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3164
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35458_length_3164_cov_4.273078"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1362)
                     /gene="leuS"
                     /locus_tag="PKH07_06560"
     CDS             complement(<1..1362)
                     /gene="leuS"
                     /locus_tag="PKH07_06560"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HNT34640.1"
                     /translation="MSEVRQESCFDHAAIETKWQQYWDQHGTYRVNLHEAANPFYNLM
                     MFPYPSAEGLHVGNVFAFTGSDIYGRFMKLQGYDVFEPIGFDAFGMHSENFAIKQGLH
                     PATLVPNNIRNFRKQLKRIGAMFDWDHEVSTTEAPYYKWTQWILIQLYKQGLMERKKG
                     NVNWCPSCQTVLANEQAEGGHCERCDTPIEQRNIEQWYFLISKYAQRLLDNLSWIDWS
                     EVTRKAQENWIGRSEGAEIDFGIDGSTEKIRVFTTRPDTLFGATYVVLAPEHPLVEML
                     TPADYRATVAGYREETSRKNRLERTDLNLEKTGAFIGAYAVNPANDQKIPIWISDYVL
                     MEYGTGAIMAVPAHDERDFAFASKFGLPIPCVISEDGIAREMTEAYTGEGTMVNSGQF
                     SGTPSASGKKAVTEWLQKRGLGEERITYRLRDWCISRQRYWGPPIPIIYCDKCGVVPV
                     PEDQ"
     gene            1737..2042
                     /locus_tag="PKH07_06565"
     CDS             1737..2042
                     /locus_tag="PKH07_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020249274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HNT34641.1"
                     /translation="MNSEIEGHLESLKQFCQKWHIRELSLFGSALRPEEFNSESDLDF
                     LVSFGQEASWDLFDLVTMKQELESNLGRPVDLVEKEALHNPWRRREILRTREVIYEG"
     gene            2032..2397
                     /locus_tag="PKH07_06570"
     CDS             2032..2397
                     /locus_tag="PKH07_06570"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HNT34642.1"
                     /translation="MKADPRDMARLWDMIDCAKAIMQFIEGRCFEDFVSDRMLRNAIE
                     RNLEIIGEAARNVSAETRNRLTDIPWTSVVGLRNIIAHEYGEIRYERLWSICENRLPQ
                     LIQSLQSVEVTKDLSSDEN"
     gene            2470..>3164
                     /locus_tag="PKH07_06575"
     CDS             2470..>3164
                     /locus_tag="PKH07_06575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNT34643.1"
                     /translation="MDCTRLLRWNCKTFITIVGVSLCVISVVSAQPVRDDSDIVATFR
                     GGTLTLGQVRAHIFMTKTKELAFSKDLVMERVWSDPQRRMRGIETVAFDTMLGTYARQ
                     NNIPLSAEQEPFYKAERDLLFSRMYYEEKVKPVIEQERATFKPGYRSYYESHLNDYRL
                     PDRYSYRQVVLSASSEAGWEQLTKSKLEDALQRIVNGEDFREIAQGISSATESPKGSV
                     IGPVESTEVEPEFR"
BASE COUNT          765 a          798 c          848 g          753 t
ORIGIN      
        1 ctgatcttcg ggcacaggca ccacgccaca cttgtcacaa tagatgattg gaatcggcgg
       61 tccccaatag cgttgacggg agatacacca gtcgcgcagt cggtaggtga tccgttcctc
      121 acccaaaccc ctcttctgga gccactcggt aactgccttt ttgccagacg cagaaggtgt
      181 tcctgagaac tgccccgagt tgaccatggt accttcgccg gtgtatgcct cggtcatctc
      241 gcgagcaatc ccgtcttcgg aaatcacgca gggaataggc agaccaaact tggacgcaaa
      301 cgcgaaatct ctttcgtcgt gcgctggaac tgccatgatt gcacccgttc catattccat
      361 cagaacgtaa tcggagatcc aaattggaat cttctgatcg ttggctgggt tgacggcgta
      421 ggcgccaatg aaagcgcccg tcttttccaa attcagatcg gtgcgttcca ggcgattctt
      481 tcgagaggtc tcttctcgat agcccgcgac cgtcgcacgg tagtccgcgg gagtcaacat
      541 ttccaccaga gggtgctcag gagccaacac aacataggtt gcaccgaaca gagtgtccgg
      601 tcgagtcgta aagacacgga ttttctccgt tgatccgtca ataccgaagt cgatctcagc
      661 gccctcggag cgtccgatcc agttctcctg ggcttttcga gtcacctctg accagtctat
      721 ccatgaaaga ttgtccagga gccgctgggc atatttggaa atcaggaaat accattgctc
      781 gatattgcgc tgttcaatcg gggtgtcgca gcgttcgcag tgtccgcctt cggcctgctc
      841 gttggcaaga accgtctggc aggaagggca ccaattgacg ttgccttttt tgcgctccat
      901 caaaccttgt ttgtaaagct gaatcagaat ccactgtgtc cacttgtaat agggggcttc
      961 ggtggtcgag acctcgtgat cccagtcaaa catggctccg atgcgcttga gttgcttacg
     1021 gaaatttcgg atgttgttgg gaaccagagt tgcggggtga agtccctgct tgatggcgaa
     1081 attctcactg tgcattccaa aggcgtcgaa cccgatcggt tcgaagacat cgtatccctg
     1141 cagtttcatg aaccgtccat agatgtccga gccggtaaac gcaaagacat tccccacatg
     1201 gagtccttcc gcggacggat agggaaacat catcaaattg tagaacggat tggccgcctc
     1261 atgaaggttg acacggtaag ttccgtgttg atcccaatac tgctgccact tggtttcgat
     1321 tgctgcatgg tcgaaacacg attcctgcct gacttcgctc atcgtgaccg tcctctcact
     1381 gctcttatcc aagagcgttt tcaaacaaca gaatgtaaac ttatcagaac gaccggatct
     1441 tcgcaaacga ctcagactgg aaagaatcgc tcgggtgaag tatattattg cagaagtctc
     1501 aggagtacgt tcccctgcag actgcgtcat aaatgacgaa aggtgatagt atatcattct
     1561 gagtgagtta gcacctgcgg gcgtgtgaac tgtgcccact cccgcaaacg cttgctcatt
     1621 ggaaggaaca ggcttcgagg tagggttgcg cttcagagtg tgcgagcttg ttcgcgatag
     1681 actcggacaa ctccgccacg gctgtaggtt gtgcttcagc caatgagagc gtattcatga
     1741 actcagagat tgaaggtcat ttggagtcgt tgaagcaatt ctgtcagaag tggcatatca
     1801 gggaactgtc actgttcggt tcggcgcttc gaccggaaga gttcaactct gaaagtgact
     1861 tggatttcct ggtgagtttt gggcaggagg cttcctggga tctcttcgac ttggtcacca
     1921 tgaaacagga gttggaaagc aaccttggaa gacctgtgga tctggttgag aaggaagcgt
     1981 tgcacaatcc ctggcggaga cgcgaaatcc tgagaacgcg cgaggtcatc tatgaaggct
     2041 gatcccaggg acatggctcg actgtgggat atgatagact gcgccaaggc aatcatgcag
     2101 ttcatagaag gcaggtgctt cgaggacttt gtctcagatc gcatgttgcg caacgccatc
     2161 gaaagaaatc ttgagatcat tggagaagcc gcgagaaacg tctctgccga gactcggaac
     2221 agacttacgg atattccttg gacctctgtt gttggtctga ggaatatcat tgcccatgaa
     2281 tacggagaga tacgatatga gagactgtgg agtatttgcg aaaacagact tccccaactg
     2341 atccagagtc tgcagtcggt ggaagtcacc aaagatctct catcggacga gaactaagac
     2401 tattgtatag tggagttgcg cttcagagtg tgcgcgattt cccagcacaa gaaaagagag
     2461 gattttgcca tggactgtac cagattgtta cgttggaact gcaagacttt catcaccatt
     2521 gtcggagtgt cgctgtgtgt gatctctgtt gtcagcgcgc agccagttcg tgacgattct
     2581 gacattgtcg ccaccttccg cggtggaacg ctcacgcttg gtcaggttcg tgctcacata
     2641 ttcatgacca aaacgaagga actcgctttc agcaaagatc tcgtcatgga gcgagtctgg
     2701 agtgatcctc agaggcggat gcgtgggatc gagacagttg ccttcgacac catgctcgga
     2761 acctatgccc gacagaacaa tatcccccta agtgcggagc aggaaccctt ctacaaggcg
     2821 gaacgggatt tgcttttcag tcggatgtac tacgaagaga aggtcaaacc ggtcatcgaa
     2881 caggaacgtg ccaccttcaa gccagggtac cggagctact atgagagcca tttgaatgac
     2941 taccgtcttc cagaccgcta ttcgtataga caggtcgtcc tgtcagcctc gtctgaagcg
     3001 ggttgggaac aacttacgaa gtcaaaactg gaagacgccc ttcaacgcat cgtcaacgga
     3061 gaggatttcc gtgaaatcgc ccaaggcatc tcatcagcga cggaatcccc caagggatcc
     3121 gtcataggcc ccgttgagag cacggaagtt gagcccgagt ttcg
//
LOCUS       DAOQUC010000310         3161 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_35514_length_3161_cov_3.295557, whole genome shotgun sequence.
ACCESSION   DAOQUC010000310 DAOQUC010000000
VERSION     DAOQUC010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3161
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35514_length_3161_cov_3.295557"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="PKH07_06580"
     CDS             <1..315
                     /locus_tag="PKH07_06580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34644.1"
                     /translation="RDGWCEVFPDERPCVWVRAYRNLGAEEAEKQFADQTILPRNHAL
                     DQTASWLNYYAERDHEAHYGIAPEERPPSLFDQPEPQKQAAVDEPSAMPEAPPDQNQQ
                     QV"
     gene            complement(481..1266)
                     /locus_tag="PKH07_06585"
     CDS             complement(481..1266)
                     /locus_tag="PKH07_06585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34645.1"
                     /translation="MDISIFTLTMGREKYLRRLIETIRNNHRRSLSLEHHIAFQGVKP
                     SPELESFLSEYRDSTGCNLVLHLWPENCGTGEACNRLVPLLEGRIIIKFDEDAALCSE
                     DFFTHMMEINRVEPDVVFSPFPVGLIGAVGGPMSTDRYVKYGAETDTYYTFRVVRHVG
                     GFARITPAHTIKSYHWPNDLNECTSGIDDVNFSKYCCQTGIKMCYLENGLIVEHQESS
                     LGQLERYKAYFTKQTIAAQKPLPIRAIRKARRIAARFVNLFRG"
     gene            complement(1313..2773)
                     /gene="hisS"
                     /locus_tag="PKH07_06590"
     CDS             complement(1313..2773)
                     /gene="hisS"
                     /locus_tag="PKH07_06590"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HNT34646.1"
                     /translation="MTGETKRALVNPQTLKGFQDFLPEAMILRNQVAQRIKSVYESYG
                     FLPIDTPILEHLASLIGTGGEETNKELFRLESPENEAVAMRYDLTVPLARIVAQYRDT
                     LKLPFRRYHFGPVFRADKPGFGRFRQFTQFDIDVVGTESVAADAEIIAAMCEVMRAVG
                     ICSDDEVRDFEIRINNRKLMDALLQGFGLRSMELQKHVLRVIDKLKKVGLDNVRKELG
                     QGRVDESGDPIKGVGLSPDIIEKVLAFISIRGSSRHGVLEEVQKHLSDSEASRTALSE
                     MRTLADCLDALHVRESEALFDPSLARGLDYYTGPIYEAYLLAAPEFGSVIGGGRYDRL
                     VERFLDVAVPGTGASIGFDRFVSALEKLGKLPAQAAVTKALLITLGDVQMTDVLGCAK
                     ELRNAGIPTEVYLGEGKAGMKTQLSFANTRRIPIAVILGEDEVKAGKVSIKDLQAGFE
                     QRAEIKDHDAYRKAGKSGQVTVDRSAMVETVKSLLG"
     gene            complement(2841..2918)
                     /locus_tag="PKH07_06595"
     tRNA            complement(2841..2918)
                     /locus_tag="PKH07_06595"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2881..2883),aa:His,seq:gtg)
     gene            complement(2963..>3161)
                     /locus_tag="PKH07_06600"
     CDS             complement(2963..>3161)
                     /locus_tag="PKH07_06600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34647.1"
                     /translation="APEENVPKLLKLIPLEASFTVCEGNDCNKLIDIVIEKQLARDMI
                     PVLARNGTKRISVETLAILYE"
BASE COUNT          750 a          903 c          776 g          732 t
ORIGIN      
        1 cgcgacggat ggtgtgaggt ctttcccgat gaacgcccct gcgtctgggt tcgcgcctat
       61 cgaaacctcg gtgcagaaga agcagagaag cagttcgccg accagacgat ccttccgaga
      121 aaccatgcac tcgatcagac tgcgtcctgg ttgaactact acgcggaacg ggatcatgag
      181 gctcactatg gcatcgcacc tgaagaacgt ccgccttcac tgtttgacca accagaaccg
      241 caaaaacagg ccgcagttga tgagccatcg gcgatgcctg aagcaccacc cgatcagaat
      301 caacagcagg tctgaagggc tgtttcagca tactggctca gttatgctgg tgatacagac
      361 aggctatgct tccgaagcaa cgccaccaaa acccgctgtc tgaccgtttc cgcttcggca
      421 gcggcgcaga gcctctgcta ccgaagcatg cctccacccc tccgaaactg agaggctgtc
      481 tcagccgcga aacaggttga caaaacgagc ggcaatgcgc cgagctttcc ggatcgctcg
      541 aatcggaaga ggtttctgtg cagcgattgt ctgtttggta aaatatgcct tgtagcgttc
      601 cagctgccca aggctgcttt cctgatgctc gacaatgagg ccgttctcca ggtagcacat
      661 cttgatcccc gtctggcagc agtacttgga gaaattgaca tcgtcaatgc cacttgtgca
      721 ttcattgagg tcattcggcc aatggtaaga cttgatcgta tgtgcaggcg tgattcgggc
      781 aaagccccca acatgtcgca cgacgcgaaa ggtgtagtag gtatcggtct cagcaccgta
      841 cttcacgtaa cggtctgtgg acatgggtcc gcccacggca ccaatcaaac caactgggaa
      901 gggcgagaaa acaacatccg gctcaacgcg attgatttcc atcatgtgtg tgaagaaatc
      961 ttccgaacag agagcggcat cttcatcgaa cttgatgatg atgcgacctt cgagtaaggg
     1021 aacaagtctg ttacaggctt cccccgttcc gcagttttcc ggccagagat gcagcacgag
     1081 attgcatcct gtgctgtccc gatattccga aaggaatgac tccagttcgg gagagggctt
     1141 aacaccctga aaggcgatgt gatgttcgag tgagagggat ctcctgtggt tgttcctgat
     1201 ggtctcgatg agccgcctga gatacttctc tcgtcccatc gtgagcgtga agatactgat
     1261 atccatcaga tctctctatc gtctcccctg aagagacaca aattggaaac aactatccga
     1321 gaagactctt gacagtctcc accatcgcag aacggtcaac agtcacctgg ccactcttgc
     1381 cggctttgcg gtaagcatca tgatccttga tctctgcacg ctgctcgaaa cctgcctgca
     1441 gatccttgat ggacactttg ccagccttga cttcatcttc acccaggatg acagcgatgg
     1501 gaatgcgcct cgtattcgcg aaagacagtt gggtcttcat tccggctttg ccttcgccaa
     1561 ggtaaacctc ggtcgggata cccgcgttgc gaagttcctt ggcacacccc aaaacatcgg
     1621 tcatctgaac gtcacccaga gtaatgagca gtgcttttgt cactgccgcc tgagcgggaa
     1681 gtttgcccag tttctcaagc gccgagacga atcgatcaaa tccgatggat gcccctgtgc
     1741 caggtaccgc gacatccagg aaacgctcta ccagacggtc gtaccgtccg ccgccgatga
     1801 cggagccgaa ctcaggagct gccagtagat aggcttcata gatcggtccg gtgtagtagt
     1861 ccaagcctcg cgccaggctg ggatcaaaca aggcttcaga ctcccgaaca tggagcgcat
     1921 ccaggcagtc agccaaagtc ctcatctccg acaatgcagt ccggctggcc tccgaatcgg
     1981 aaaggtgttt ctgaacctct tccagaacac catggcgcga actccctctt atcgagataa
     2041 atgccaggac tttttcgatg atgtcgggac tcagtccaac gcctttgatg gggtcacccg
     2101 actcatctac acggccttgc cccaactctt tcctgacatt gtccaagccg accttcttga
     2161 gtttgtcaat gacacgcaga acatgcttct gcaactccat actgcgaagg ccaaatccct
     2221 gcaacaaagc atccatcagt tttctgttgt tgatgcgaat ctcaaagtcc cgaacctcat
     2281 catcgctgca aatccctacc gcacgcataa cttcacacat ggcggcgata atctcggcgt
     2341 cagccgccac cgactcggtc ccaacgacat caatatcaaa ctgtgtgaac tggcgaaatc
     2401 ggccaaatcc tggtttgtcc gctcgaaaca caggaccaaa atgatagcgc ctgaatggca
     2461 acttcagtgt gtctcgatac tgggcaacga tcctcgcgag cggaacggtc agatcgtagc
     2521 gcattgccac cgcttcattc tcaggactct ccaaacggaa cagttccttg ttggtctctt
     2581 caccgcctgt tccgatcaga ctcgccaagt gctccagaat cggtgtgtca atgggcaaga
     2641 aaccgtaact ttcgtagaca ctcttgatcc gctgggcaac ttgattgcga agaatcatcg
     2701 cctcgggaag gaagtcctga aatcccttca gcgtctgagg gttcaccaat gcccttttcg
     2761 tttcgccggt catggtctct ttgctcctcg ctgtaatgct ctcatgtcag atgttggatg
     2821 gcggtgtatg gaaggaaagg tggtaccggg aatgagactt gaactcatga cctcctgatc
     2881 cacagtcagg cgctctaaac caactgagct atcccggcac cgttgttcat atccctacca
     2941 cacttttcca tcggatcgca acctattcgt atagaattgc gagcgtttcg actgaaattc
     3001 tcttggttcc atttcgagcc agcaccggaa tcatgtcccg agccagctgc ttttcaatga
     3061 cgatatctat caacttgttg cagtcattac cttcacagac ggtgaaactg gcttccaaag
     3121 gaatcagttt cagcagtttt ggaacgttct cttccggagc g
//
LOCUS       DAOQUC010000311         3160 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_35534_length_3160_cov_3.090821, whole genome shotgun sequence.
ACCESSION   DAOQUC010000311 DAOQUC010000000
VERSION     DAOQUC010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3160
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35534_length_3160_cov_3.090821"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1738
                     /locus_tag="PKH07_06605"
     CDS             <1..1738
                     /locus_tag="PKH07_06605"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HNT34648.1"
                     /translation="EKYVGGRGFGAKLVWDNLRRHNFKLDPLGPENLIAIAPGPLSGL
                     YLPSSGKNSFVAISPATGLYADSSLGGHFGVELRQAGFDLLSITGKTDVLSILFIDNS
                     DISIIPMPELKGKTSLETEGMIKERLGTHAIHVATIGVAGENLVRFACINSDWSRNAG
                     RTGMGAILGSKNLKAIAVRGHHDLPVHDLKALVEESNRAIKYMKDHKYFRLWQQQGLM
                     NVIDYANEKGILPTYNFKDSTFSRSNKINGQVMLDHYKIGDSACFACPMCCGNICLVK
                     NGKFTGTVTEGPEYESCAMLGSNVGIDNFSAVLAANRLCDELGVDTISTGSIVAAVTE
                     GYEKAIISLEDLDGREITWGDEDAILELIRKIAYREGIGDTLAGGSKAIIARWPEMAK
                     VVLQVKGLEQSAYDSRGGVSMGLAYATCDIGAHHTRAWTIAKEIEEGSNWTNEDRVKT
                     VIYHQKLRPLFDMLGVCRLPWIELGLNERHYENFYTHVTGKKMTLEELLELSNDLYNL
                     TRLINSKMGVSRKDDSLPYKNYACAPLTGPNAGKSINQEEFQKLLSLYYKARGWDENG
                     QPPADTEQRFMD"
     gene            complement(1741..2652)
                     /locus_tag="PKH07_06610"
     CDS             complement(1741..2652)
                     /locus_tag="PKH07_06610"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HNT34649.1"
                     /translation="MQTAAEGGKGDDFALELARMKLRAKVEQIKNLPTLPQVVSHLLS
                     MMVEDTCSASQLSQEISKDQSLTAKILKVVNSAYYGFHRQISALADAVVILGLEEIQR
                     LTATIAVFGMVHIKPIGDFSPRALWEHSVATAAGAEMISNLTPHPSKGAFVCGLLHDI
                     GKVVFDQFFNPQWQAVVLKAQEKSLWIGIPEQKEFGTHHGEIGAWLTERWSLPPWIVE
                     GIRFHHETVEPHLISSASNLWLVVQLADRIAREENYGSGGDKQMPPAFPGIEEYLSIQ
                     PEQIEDIRNRLKDQMERLRTQWNFLGA"
     gene            2768..>3160
                     /locus_tag="PKH07_06615"
     CDS             2768..>3160
                     /locus_tag="PKH07_06615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34650.1"
                     /translation="MSDKSTTATEQKGRRGFLRRGLIGLLGGLFTRKAMAQQQDLPPA
                     TGMKGDILHTHFRKGNPPVQPLDTMVLFERGDDNNDRAMTHEVLSLIHEEKGKNSYPW
                     TVYASLETHHEVGDACVLCSRLHKYGPGW"
BASE COUNT          765 a          820 c          873 g          702 t
ORIGIN      
        1 cgagaagtac gtaggcggac gcgggtttgg agccaaactg gtctgggaca atctacgccg
       61 tcacaatttc aaactggatc cgctgggacc ggaaaatctt atcgccatcg caccgggacc
      121 cttgtcaggt ctttatcttc cgtcgtcggg aaagaactcc tttgtggcga tctctccggc
      181 gaccggactg tacgcagact cgtcgctcgg tgggcacttt ggtgtcgagt tgcgccaagc
      241 cggatttgat ctgctttcga tcacaggaaa gacggatgtg ctctcgattc tgttcattga
      301 caattcggac atcagcatca tccccatgcc cgaactgaaa gggaaaacct ctctggagac
      361 agagggcatg atcaaggagc gtctgggcac tcacgcgatt catgtggcga ccatcggtgt
      421 tgccggtgaa aacctcgtcc gattcgcctg catcaacagc gactggagcc gaaatgccgg
      481 aagaacaggg atgggcgcta tcctgggttc caagaacttg aaagccatcg ctgttcgggg
      541 acatcacgat ctccctgttc acgatctgaa agcactggtg gaggagtcca atcgtgccat
      601 caagtacatg aaggatcaca agtacttcag gctctggcag cagcagggct tgatgaatgt
      661 tattgactat gccaatgaga aaggcattct ccccacctat aacttcaagg acagcacctt
      721 ctctcgctcg aacaagatca acggtcaggt catgctcgac cattacaaaa ttggagacag
      781 tgcctgcttc gcctgtccca tgtgctgcgg gaacatctgc ttggtgaaga acggcaagtt
      841 cacgggcacc gtaaccgaag gtcctgaata cgagtcctgc gcgatgttgg gatcgaatgt
      901 gggtattgac aatttctcgg cggtgcttgc cgccaaccgc ctgtgtgatg agctcggagt
      961 ggacacgatc tctacaggca gcatcgtcgc tgcggtcacg gagggttacg aaaaggcaat
     1021 catcagcctt gaagatctcg acggtcgtga gatcacatgg ggtgatgagg atgcgattct
     1081 ggagttgatt cggaaaatcg cctatcgcga aggcattggc gacactcttg ccggtggctc
     1141 gaaagccatc attgcgcgct ggcctgagat ggccaaggtt gtacttcagg tcaaagggct
     1201 ggagcagtcg gcctatgaca gtcgtggagg agtctcgatg ggattggcct atgcgacctg
     1261 cgacattggt gcgcatcata cgcgagcttg gacgatcgcc aaagagatcg aggaaggaag
     1321 caactggact aacgaggatc gagtcaaaac ggtcatctat catcagaagc ttcgaccgtt
     1381 gttcgatatg ctgggtgtgt gtcggttgcc gtggatcgaa ctcggtctca atgagcgaca
     1441 ctacgagaat ttctataccc acgtcacggg caagaagatg acgctggagg aattgctcga
     1501 gctgtccaat gacctctaca atctgactcg gttgatcaac tccaagatgg gcgtttcccg
     1561 aaaggatgac tcacttccct ataagaatta cgcctgcgcg cccttgaccg gtccaaatgc
     1621 aggaaagtca atcaaccagg aagagttcca gaaactcctt tccctctact acaaagcacg
     1681 gggatgggac gagaacgggc aaccgcctgc tgacacggag cagcggttca tggactgaat
     1741 ctaggcgccc aggaaattcc actgggttcg aagtcgctcc atctgatcct tgagacgatt
     1801 gcggatatcc tcaatctgtt ccggctgaat gctcaggtac tcttcaatgc ctgggaacgc
     1861 agggggcatc tgtttgtcgc caccagaacc atagttctcc tcacgcgcga tccgatctgc
     1921 caactgaacc accaaccaga gattggatgc cgaggagatc aagtgcggtt cgactgtctc
     1981 gtggtgaaac cgaatgcctt cgacaatcca aggtggaagg ctccagcgtt ctgtcagcca
     2041 ggctccgatc tcaccatggt gggttccgaa ttccttctgc tcgggtatcc caatccagag
     2101 agacttctcc tgtgctttca gaacgaccgc ttgccattga gggttgaaga actggtcgaa
     2161 aacaaccttt ccgatgtcat gcagcagacc gcagacaaat gcaccctttg agggatgagg
     2221 agtgaggttg gagatcatct cagctccggc ggcggtcgca acggagtgtt cccacaacgc
     2281 gcgaggtgag aaatccccga tgggcttgat gtgaaccatc ccgaagacag caatggttgc
     2341 ggtgagtctt tggatttcct caagtcccag gatgacgact gcgtcagcca atgcgctgat
     2401 ctgtcggtgg aagccgtaat aggcggaatt gacgaccttg agtatcttgg ccgtcaatga
     2461 ctgatctttg gatatctcct ggctcaattg gctggcagaa caggtgtctt ccaccatcat
     2521 actaagcaag tggcttacta cttgtggcag ggtcggcaga ttcttgatct gctccacctt
     2581 tgcacggagt ttcattcggg cgagttccaa ggcgaaatcg tcgcctttgc cgccttccgc
     2641 agctgtctgc accaatgtat caaccctcca tcactcaatt ggtcaggaat tatagcaaga
     2701 acccgatgga gcgtcaaaag caatcatgat agtctgggag tgaccaaatc atgccaagga
     2761 ggcaatcatg tcagacaaaa gcacaaccgc aacagagcaa aaaggcagac gtggatttct
     2821 gcggcgtgga ttgatcggac tactgggcgg tctgttcacc cgaaaggcaa tggcacagca
     2881 gcaggacttg ccacccgcga cagggatgaa aggtgacatc cttcatactc acttccgaaa
     2941 agggaatccg cccgttcaac ctcttgacac catggtgctc ttcgaacgtg gtgatgacaa
     3001 caacgaccgt gccatgactc acgaagtgct gtcactcatt cacgaggaga aagggaagaa
     3061 ttcctatccc tggacggtct acgcgagttt ggaaacacac catgaggttg gagacgcctg
     3121 tgtgttgtgc tctcggcttc acaagtacgg tcctggctgg
//
LOCUS       DAOQUC010000312         3158 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_35556_length_3158_cov_3.537867, whole genome shotgun sequence.
ACCESSION   DAOQUC010000312 DAOQUC010000000
VERSION     DAOQUC010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3158
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35556_length_3158_cov_3.537867"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1160
                     /locus_tag="PKH07_06620"
     CDS             <1..1160
                     /locus_tag="PKH07_06620"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HNT34651.1"
                     /translation="YALTQVNPDVLNRIRKHLDAQSLMIQYLFYEDNLFLFAVTSDKL
                     DYRTVKIDKKNLESRITYLRDLLRISFRFYPASVQSRIEGDPKWKSSYQETFLMPIAQ
                     VGNRLYGELIAPLGDLLAGKTRLVVIPNQGLHYLPFAALVISQEGDRTRYLVEDYEIL
                     KQGSLTLLDFARGGAVEEPVNVLAIGNADGTLPNAQEEAERLKTVFATANVLLRDQAA
                     EDVFKGLCSEKTILHLATHAFLDPGDLDKCYIKLFPAWDKGEDGCLWRREVLDLTLDK
                     VSLVTLSACETGIGEHRLQGDEVYGMEQSFVKAGAETVISTLWPVEDKATGLFMQTFY
                     ENLVKSLAESQGERRMSKLTSLRKAQLTLLNDPQYFHPYFWAPFVMTGEAE"
     gene            1163..2479
                     /locus_tag="PKH07_06625"
     CDS             1163..2479
                     /locus_tag="PKH07_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008006281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BNR repeat-containing protein"
                     /protein_id="HNT34652.1"
                     /translation="MKYVLILISGCVFCRVASAEVTQEILDVTPVWSGHPVGFCLLTK
                     GSRQFAAFYDAERNMTVASRVLDSTEWLFVRLPEKLGWDSHNAVTMVLDDDSRIHLTG
                     NMHAVPLVYFRTREPLDITTFERLDRMVGDREAKCTYPSFMRGVNNEFIFTYRDGSSG
                     NGDQIYNVYDHQTQLWKRLLDQPLLSGKGLMNAYLHGPVKGPDGLHHLCWVWRDRSGC
                     ELNHDLSYARSRDLIHWETSSGEPLELPLTIDNAEIVDPVPVRGGMINGNTRIGFDSK
                     KRPVISYHKFDQQGLTQAYNARLEDGTWAIYQVSNWDYRWDFSGGGTIIFEVRVNSVR
                     VQTDGRLSQTFSHIKYGTREWILDEETFSVIEEREISPASPTPSTPSESEAAVLASRR
                     CGDSGSSDDPSVHYVLEWKTLPQNRDRPREGELPSPSMMRILKIRK"
     gene            2492..>3158
                     /locus_tag="PKH07_06630"
     CDS             2492..>3158
                     /locus_tag="PKH07_06630"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HNT34653.1"
                     /translation="MRICFCVVLFSMFGSVSTAEPVVMDGKTPRLQNDFDRPTTVDVF
                     CNPLEVSLGDPQILQDGDMYYLYGTTYDTLGFEVWSSSDLVHWRKHGFCWQKSGTTWA
                     QRYLWAPEAIKVGGTYYLFYTGATASDPRLRVCVASSTSPLGPYADLQAPLVGMGEVN
                     HFDPSVYRDPVTDDCFLYYTVDTTQSGSNYSEIRCVRLGSDMKSLIGFPVVCIKGDQN
                     WEYT"
BASE COUNT          708 a          815 c          853 g          782 t
ORIGIN      
        1 tctatgctct aacacaggtc aatccggacg ttctgaaccg catcaggaaa caccttgacg
       61 cccagagcct catgatccag taccttttct atgaagataa tctctttctg ttcgctgtca
      121 cgtcagacaa actggattat cgaaccgtca agatagacaa gaaaaatctg gaatcacgca
      181 tcacttattt gcgcgacctt ctcaggattt ccttccgttt ctatccggcc tctgtacaaa
      241 gccgtatcga aggtgatccc aaatggaaga gttcctatca agagaccttt ctcatgccga
      301 ttgcccaggt cggcaatcgt ctctacggtg aactcattgc gccgttgggt gatctgttgg
      361 cggggaaaac tcgtttagtg gttataccga accagggcct gcactatctg ccttttgctg
      421 cgttggtgat atctcaagaa ggcgatcgaa cgcggtacct cgtcgaggat tatgagatcc
      481 tgaagcaagg aagcttgacc cttctcgatt tcgcacgtgg cggtgcggtt gaggaaccgg
      541 tcaacgttct tgccattggc aatgcagatg gcacactgcc gaacgctcag gaagaggcgg
      601 aacgtctgaa gacagtcttc gccaccgcaa atgtgctgct cagggatcag gcggccgagg
      661 atgtgttcaa gggtctgtgt tcagagaaga ccattctcca tctcgcaaca catgcatttc
      721 tggatccagg cgacttggat aagtgctaca tcaaactttt tcccgcatgg gataagggtg
      781 aagacggctg cctgtggcga cgggaagttc tcgatctcac actggacaaa gtgagcctag
      841 tcactctgtc cgcttgtgaa acaggcattg gcgagcatcg gcttcagggc gacgaggtct
      901 atggcatgga acagagtttc gtcaaggcag gtgcggagac ggtcatttca acactctggc
      961 ctgttgaaga caaggccacc ggtcttttca tgcaaacctt ctacgagaac ctggtcaagt
     1021 ccctcgccga gagccaggga gagagacgga tgagcaagtt gacttcactt cgcaaggcgc
     1081 agttgacgct gctcaacgat ccccagtatt tccatcctta cttttgggca ccttttgtta
     1141 tgacgggcga agcggagtaa ccatgaagta cgttctgatt cttatttctg ggtgtgtttt
     1201 ctgtcgtgtc gcaagcgctg aggtgactca ggagatcctt gacgtcacgc cagtctggtc
     1261 aggccatcct gttgggttct gtcttctgac caaaggcagc aggcagtttg ccgcgtttta
     1321 tgatgccgag cgaaacatga cggttgcttc gcgagtcctg gacagtacag agtggttgtt
     1381 tgtccgactg cctgagaaat tggggtggga cagccacaac gcagtcacga tggtgctcga
     1441 tgatgatagt cggattcatc tgacaggcaa catgcatgcg gttcctctgg tctacttccg
     1501 aaccagagag cctttggata tcacgacgtt cgaacgactc gatcgcatgg tgggcgatcg
     1561 tgaggccaaa tgtacttatc cctccttcat gcgcggcgtg aacaacgagt tcatattcac
     1621 ctatcgggat ggttccagtg gcaatggtga tcagatctac aatgtttacg atcaccagac
     1681 gcagctgtgg aaaagactcc tggatcagcc actgctttcg ggaaagggtt tgatgaacgc
     1741 gtaccttcat ggccctgtga aggggcctga cggtctgcac catctttgct gggtctggcg
     1801 agaccgatcg ggttgtgagc tcaaccacga tctgtcctat gcccgcagtc gagacctgat
     1861 tcattgggag acaagttccg gagagccgct tgaactccct ctcaccattg acaacgcgga
     1921 gattgttgat ccggtgccgg ttcgcggcgg tatgatcaac ggcaatacca ggatcggttt
     1981 tgactccaag aagcgtccgg tgatcagtta tcacaaattc gaccagcagg gattgaccca
     2041 ggcatacaac gctcgacttg aggacggaac ctgggcgata taccaggttt ccaattggga
     2101 ctaccgatgg gacttcagtg gcggtggcac gatcatcttc gaagtcagag tgaactctgt
     2161 ccgcgttcag accgacggca gactctccca gacattcagc cacatcaagt atggcacgag
     2221 ggagtggata ctcgacgaag aaaccttctc tgttattgag gagcgcgaaa tctctcctgc
     2281 ctctccgacg ccgtccacac ccagcgagtc ggaggccgca gttctggcat cccgacgttg
     2341 tggcgattca ggttcttccg atgatccttc ggttcactac gttctcgaat ggaagacttt
     2401 gccccagaac cgtgacagac cgagagaagg cgaactccct tctcccagta tgatgcggat
     2461 tctcaagatt cggaaataga ggatccatga tatgcgtatt tgtttctgtg ttgttttgtt
     2521 ctccatgttc ggaagcgttt ccacggcgga acccgttgtc atggatggca aaacgccgcg
     2581 ccttcagaat gactttgacc ggccgacaac ggtagatgtt ttctgcaatc ctctggaagt
     2641 gtccttgggt gacccgcaga ttctgcaaga tggggacatg tactacctct acggcacaac
     2701 gtacgacact cttgggttcg aagtctggtc atcctctgat ttggtgcatt ggcgcaagca
     2761 cggattctgt tggcagaaaa gcggtaccac ctgggcgcaa cgctatctgt gggcgccaga
     2821 ggcgatcaag gttggtggta cctactatct tttctacaca ggcgcaaccg catccgaccc
     2881 gcgacttcgc gtgtgtgtcg cctcaagcac ctctcccctc ggtccctatg cagatcttca
     2941 ggctccgttg gtggggatgg gtgaggtgaa ccatttcgat cccagtgtct atcgtgatcc
     3001 ggtcaccgat gattgttttc tttactacac ggttgacacc acacagtcag gatcaaacta
     3061 cagcgaaatt cgttgtgttc gtcttgggtc agacatgaag tcgctcatcg gttttcccgt
     3121 cgtttgcatc aagggtgacc agaactggga atacacgg
//
LOCUS       DAOQUC010000313         3152 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_35661_length_3152_cov_3.091702, whole genome shotgun sequence.
ACCESSION   DAOQUC010000313 DAOQUC010000000
VERSION     DAOQUC010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3152
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35661_length_3152_cov_3.091702"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(126..701)
                     /locus_tag="PKH07_06635"
     CDS             complement(126..701)
                     /locus_tag="PKH07_06635"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNT34654.1"
                     /translation="MQLQRISGGTALFASGCLFCYVFLRVNEMKIAEKLSLLRVKKGL
                     SYGRLAQLARISPSTYLQWEQGVAPTRLKLVPVLDVFSNLYGMDIPEREMRDESQALR
                     FDNMGKPSFARETVARNPGISADRGNTSTSLEPQARPETILEYEGIYSRLRVENSLTE
                     QEAQMLLYLVKGFLAENHKVTDGKRNSRRRK"
     gene            859..2823
                     /locus_tag="PKH07_06640"
     CDS             859..2823
                     /locus_tag="PKH07_06640"
                     /inference="COORDINATES: protein motif:HMM:NF021379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2079 domain-containing protein"
                     /protein_id="HNT34655.1"
                     /translation="MSVPKGWKQLLPSLLVIVFAGWNFAIAVSNKGMLLPFRAENASL
                     VSDYREVWGNIFWFVPFFITLWLVIGWWVSRDGVRSLADALERDAFRTLPLLLLVPLA
                     WFTSELGWSLSSRMLIVQIVLGVTMVLRLSEKRSSCDLHPAIAKMIIILAGFAFAIVV
                     SRFAIHRHREFHTYAFDLGMVSNIVWNTSRGRLFQTSLFPWSILGEHLAFVYVLFAPL
                     YWLWEDPRMLIIVQALLLGLSGVAFYLLSQRVLRQTAPAFCLALAYLLHPGLRGVGLT
                     EVHEVAFFPLLFFSALYVGWPDEVERRWTSGKSAAFLILLFGAVACREDTALLVAALG
                     IYLILGQNRTRLGIGIVVGALAWYILATGLVIPFFRGKPYSYTVRYAEMGGTFGGMIS
                     TAIANPVYTLRLIFSEAHRPFLWQALGSVLFVALLSGWACLMLLLALAACVLASEPQQ
                     YSLLSQYPAVWLPVVFVTVLLAMRRLAKVGVSDAQDDEKPYVQKNRLRSSLKPMPIAM
                     TLLALAVLTPPFSVVPPWKTMVNSGSWSLVYPPQPFGEDARTVLARFTDRTGSVSAQT
                     IFVPHVASRSKVFQFPDGLNTDYILVDIGQSSNSYPLTKEELRNSILTLLKQGTYQVK
                     DRSGSVCLLSLGGVPTEATGLVIRELEAGQ"
     gene            2859..>3152
                     /locus_tag="PKH07_06645"
     CDS             2859..>3152
                     /locus_tag="PKH07_06645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34656.1"
                     /translation="MKAALFGTFLILIALSIVAWLIEPPLAEHGKTPLIWTSDDNPAR
                     REQIDLFNELHPMFQVKLDPSNSDMQKVIVQSLAGVGPDLFCSYNGFQLSAYVK"
BASE COUNT          620 a          812 c          836 g          884 t
ORIGIN      
        1 aaagacgatc tgaatgttca gtttgttcac gataccgtga atatccactg ggatcgtgac
       61 gctgggaaga cgattcttca acgtatcctg cgcgatctct tcaggttttc ttgtcgcttt
      121 tcttgtcact tccgccgcct gctgtttcgt ttgccatctg ttaccttgtg gttctcagca
      181 aggaatcctt tgacgagata aaggagcatc tgagcttcct gttccgtaag actgttttcg
      241 actctgagtc ttgagtaaat cccttcatat tcgagtatgg tctctggacg cgcctgaggt
      301 tccagcgacg ttgaggtatt ccccctgtca gcagagatgc caggattcct tgcgacggtc
      361 tctctggcaa acgaaggctt gcccatgttg tcaaatcgaa gcgcctgaga ctcatcccgc
      421 atttctcgtt cggggatatc cattccgtac agattcgaaa acacatccaa aactggaacc
      481 agtttaagcc gtgttggcgc cactccctgt tcccactgaa ggtaagtgct gggggaaatc
      541 ctcgccaact gagccaagcg tccgtaagag aggcctttct tgactctcaa cagactcaat
      601 ttctctgcta tcttcatttc attcacccga aggaatacat agcagaacag acagcccgat
      661 gcaaacaatg cggttccacc tgaaatccgt tgcagttgca ctctcaatcc cctagacttc
      721 caaagtcagc aacggcatac tcctggcgac acagagcctg agagatgtgt gtcctttgtg
      781 gaacacgcat ccggagggag tgagcatagc ccgaacacct gcaggcgctg gcctgctcag
      841 aatgtgtgga atcacaacat gagcgttccc aaaggttgga aacaactgct gccgagtctt
      901 ctcgttattg tctttgcagg ttggaacttc gccattgcgg tttccaacaa gggcatgctt
      961 ctaccattcc gcgctgagaa cgcgtcactt gtttcggatt accgtgaagt gtggggaaac
     1021 atattctggt tcgtgccttt ttttattaca ttgtggctcg taatcggatg gtgggtttca
     1081 cgtgacggtg tgcggagtct ggctgatgcg ctcgaacggg atgcattcag aacactcccc
     1141 ctcctcttgt tggttcctct ggcctggttt acctcggaac tcggttggag tctttcatcg
     1201 aggatgctca tcgttcagat cgtccttggc gtgaccatgg tgctccggct ttccgagaaa
     1261 agatcttcat gcgatcttca tccagcaatc gcaaagatga tcatcatctt ggcgggattt
     1321 gctttcgcga tcgttgtctc ccgctttgcc atccatcggc atcgggagtt tcacacctac
     1381 gcttttgatc ttgggatggt cagcaacatt gtctggaata catctcgggg caggttgttt
     1441 cagacttcgc ttttcccatg gtctatcctt ggggagcacc tggcatttgt ctacgtgttg
     1501 ttcgcaccgt tgtactggct ctgggaggac cccaggatgt tgataatcgt gcaggcgctg
     1561 cttctgggtt tgagcggtgt tgccttctac cttctcagcc agagagtttt gcgacagact
     1621 gcgcccgcgt tttgtctggc cttggcttat ctgttgcatc ccgggcttcg aggtgtggga
     1681 cttaccgaag tgcatgaagt tgcgttcttc ccgcttctct tctttagcgc gctgtacgtt
     1741 ggctggcctg atgaagtgga acgaagatgg acaagtggca aatctgcggc gttcttgatt
     1801 ctattgtttg gtgcggttgc ttgtcgtgag gatacggctt tacttgtcgc ggctctcggc
     1861 atttacctaa tcctgggaca aaacagaacg cgcctgggaa ttgggatcgt ggtgggagca
     1921 ctcgcatggt acatcttggc aactgggctc gtgatcccat ttttcagagg gaagccgtac
     1981 agttacactg tgaggtatgc cgaaatgggt gggacttttg gagggatgat ctcgactgcc
     2041 attgccaacc cggtctacac acttcggctg attttctcag aagcgcacag accttttctc
     2101 tggcaggcgt tgggatcggt cttgttcgtc gcgttgttgt cggggtgggc ctgtctgatg
     2161 ctcttgcttg cgcttgctgc ctgtgttctt gcgtccgagc cccagcagta ttcacttctt
     2221 tcacagtatc cggcggtctg gttgccagtg gtctttgtga cggttcttct ggcgatgcgg
     2281 cgactcgcca aggtcggagt atcagatgcg caggatgacg aaaagcctta cgtacagaag
     2341 aaccgtttga ggtcgtctct caaaccgatg ccgatagcca tgacgttgtt ggcgctggcg
     2401 gttctgacgc cgcctttctc ggtcgttcca ccatggaaga cgatggtcaa ttctggatcg
     2461 tggtcgcttg tctatccgcc ccaacctttt ggtgaggatg cgcgaactgt gctggctcgc
     2521 tttaccgacc ggacagggtc tgtttcagcg cagaccatat ttgttccgca cgttgcctct
     2581 cgaagcaaag ttttccagtt ccccgacgga ctgaataccg attacattct cgtggatatc
     2641 gggcagtcaa gcaattccta ccctctcaca aaagaggaac ttaggaacag catcttgacg
     2701 ctgttgaagc agggaacgta ccaagttaag gacaggtcag gttctgtttg cctgttaagc
     2761 ctgggtggcg tgccgacaga ggctacgggt ttggtcattc gggagctcga agcggggcag
     2821 taacctgcgg tgcctgttaa tcttctcggg agattcgttt gaaagccgct ctttttggaa
     2881 catttctgat actgatcgcg ctttctattg ttgcgtggct catcgaacct cctctggcag
     2941 agcatgggaa gacgccgctt atatggacaa gtgacgacaa ccccgctcga cgggagcaga
     3001 tcgatctctt caatgaactt caccccatgt tccaagtcaa acttgatccg agcaactccg
     3061 atatgcagaa ggtcatcgtt cagtctctcg ctggcgtcgg ccccgatctt ttttgcagtt
     3121 acaacggatt tcagctctct gcctacgtca ag
//
LOCUS       DAOQUC010000314         3149 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_35707_length_3149_cov_3.363284, whole genome shotgun sequence.
ACCESSION   DAOQUC010000314 DAOQUC010000000
VERSION     DAOQUC010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3149
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35707_length_3149_cov_3.363284"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3149)
                     /locus_tag="PKH07_06650"
     CDS             complement(<1..>3149)
                     /locus_tag="PKH07_06650"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNT34657.1"
                     /translation="IDDLYRRTVELGLTRLGFDRLGIWLCSPENPGEILGTFGTDFSG
                     HVQDERGMRVTVPKDHFLAALVFGNQPLVTDIRFDEKEDKSAPIHCAAASIYDGERTF
                     GIIRTDNVSSHVRPFPQQSEILRLLASTLGALCSLKRAEEALRYSDEIYRTAIEKASG
                     VPYRLRYKDWRYEFVGSGAQALLGIPNDQLTLDLMSDMIQDTTIPHPDGTVSSSEYAN
                     AFLRGERDLYQVDIRIVTPDGQSKWISDTAIPLKDSRTGDVYGSLGILQDITERKHVE
                     WQLRENNRRLVALNSVAQVTLGTLSPEKLCDNLAEVFRSLMPCDACVFDLYDSGNDVV
                     SPMRLYDTVQGIFQTVPSVQAVVKPTGTMRRVIEERSPSLIHRKKPKQERAGFIGFGD
                     ASRPSASLLFAPMIAGDRVMGVLSIQSYTFNAYKESDLDLLASIAQQIGPAFEAALLS
                     DEIRTREEQERAFGRQLATLVEVTNDLSAAETLDEMIAKAVQQGHHRLGFDRLGIWLL
                     DSENLEFIQGTFGIDENGTLRDERSSRMKWHPTSVMGQVLAGESGCVLSRDAPLFDNA
                     AEVVGTGMQAVVAIQVGQTILGCLSIDNLIHGQEITEQHCEVLRLFASALGALTTSKR
                     ARQELVQTHHIYREAIQNAHGVPYLLNYTTGKYDFMGSGCEVLLGVTPDELSYDLMER
                     MVVESRPTAVGGETDPAKIVQSFIDGKVDRYQSDIRVRTPQGEEKWLSDSSISVRDPR
                     TGRVTNSIGILQDITQRKQIEEALRESEAQFRAVAETTPSALFVIQDDRFAFVNPATA
                     EITGYPMEELLQMHFWEFVHPDDQETVRQRSYSRREGGLEPNRYECRIVTKEGQTKWL
                     DISVSLVELEGRPAVITVAFEISERKRIEEEREALRRLSQALTAPLTLREIARMIADE
                     CRRFFQYDAFWFDVYDSARRLFPGTYTEDTPVNSSTPIEVETSTNVVEGMTASVVLEG
                     RAYRVDCDTDMNNGEASSSMGFVSSRTRSIICAPILWQGRGVGIISVQSHTTGKYTDS
                     DLSLLHSFADQCGGALHRI"
BASE COUNT          724 a          878 c          836 g          711 t
ORIGIN      
        1 aatccggtga agggctccac cacactgatc agcaaaggaa tgcaggagac tcaaatccga
       61 atccgtgtat tttcccgtgg tgtggctttg gacggagata attcccaccc cacgtccctg
      121 ccagagaatt ggagcacaga tgatagatcg tgtgcgtgac gacacaaagc ccatggaaga
      181 ggaggcctcg ccgttgttca tgtccgtatc gcagtccacc cgatatgccc tgccttccag
      241 gacgaccgac gcggtcatgc cttccacaac attcgtgctt gtctccactt caatcggagt
      301 ggaagagttg acaggcgtat cctcggtgta ggtgcctggg aagagccgcc gtgccgagtc
      361 gtaaacatca aaccagaaag catcatattg gaagaaacgg cgacattcat cggcaatcat
      421 ccgggcgatc tcacgcagtg tcagtggtgc agtgagggct tgagacaagc gtcgcagggc
      481 ttcacgttcc tcctcgatcc tcttgcgctc cgagatctca aaggcaactg tgatgaccgc
      541 gggtcttccc tccagttcaa ccaaactgac ggatatgtca agccacttcg tctgaccttc
      601 tttggtcacg attctgcatt cgtagcgatt gggttcgagc cccccttctc gccgtgaata
      661 actgcgctgc ctgaccgtct cctgatcgtc aggatggaca aactcccaaa aatgcatttg
      721 caggagttcc tccatcggat agcctgtgat ctcggcggtc gcaggattga caaaagcaaa
      781 ccggtcatcc tgaatcacga acagcgcaga aggtgtcgtc tccgcaacag cgcgaaactg
      841 tgcctcgctc tcacgcagtg cttcctcgat ctgtttgcgc tgtgtaatat cctgaagaat
      901 gccgatggaa ttagtgactc ggccagtgcg aggatcgcga accgagattg aggaatcact
      961 cagccacttt tcttctcctt gtggtgtccg aactcggata tccgattgat atcggtcaac
     1021 tttgccatca ataaaggact gaacaatctt ggcgggatcg gtttctccac caactgctgt
     1081 gggtctgctc tcgacaacca tacgctccat gagatcgtag gaaagctcgt cgggagttac
     1141 acccagaagc acctcacagc cggagcccat gaagtcgtac ttaccggtcg tatagttgag
     1201 aagataggga actccgtgag cattttgaat cgcctcacga tagatgtgat gggtctgcac
     1261 caattcctgt cgggcacgct tgctcgtcgt caaagcgcca agcgctgagg caaagagtcg
     1321 cagaacctca caatgctgct cggtaatttc ctgtccatgg atcaggttat caatggaaag
     1381 gcagcccagt atggtctgac cgacctgaat cgcaacaacc gcctgcatgc ctgttcccac
     1441 gacttctgca gcgttgtcga acaagggtgc atcccgactc aagacgcacc cgctttcacc
     1501 tgcgagtacc tgtcccatca cggacgtggg atgccacttc atgcggctgc tacgctcgtc
     1561 gcgcaatgtc ccattctcgt caataccaaa agttccctgg ataaactcca gattctcact
     1621 gtcgagaagc catataccca atcgatcaaa gccgagccga tggtgtcctt gttggactgc
     1681 ctttgcgatc atctcgtcca gagtctctgc cgccgaaaga tcgttggtca cttcgaccag
     1741 cgtcgccaat tgtctcccga atgcgcgttc ctgttcttcc cgagttcgga tctcatcact
     1801 cagcagggct gcctcaaatg cggggccgat ctgctgagcg atggatgcca gaagatcaag
     1861 gtcactctct ttgtaagcat tgaaggtata actctgtatc gagagaacac ccatgactcg
     1921 gtcgcccgca atcatcgggg cgaacagcag cgaagcagac ggtcgagagg catctccgaa
     1981 accgatgaat cctgctcttt cctgtttggg ttttttgcga tgaatcagcg atggggatcg
     2041 ttcctcaatg acccgacgca tggtaccggt aggcttgaca actgcctgaa cggaaggaac
     2101 ggtctgaaat atgccttgca ccgtatcata taaccgcatc ggggatacga catcgttgcc
     2161 tgaatcatag agatcgaaga cacaggcatc acaaggcatc aagctgcgaa agacctcggc
     2221 gagattgtcg cacagcttct ccggactcaa ggtgccgagc gtgacctggg caactgaatt
     2281 cagagccaca agacgccggt tgttctcccg caactgccac tcgacatgct tgcgttccgt
     2341 aatgtcctgg aggataccga gtgaaccata gacatcgccc gtccgggaat ccttcagcgg
     2401 gatggccgtg tcactgatcc atttggactg accgtcagga gtgacgatcc gaatgtctac
     2461 ctgatacaga tcccgctcac cacgaaggaa tgcgttggcg tattcgcttg aagagacggt
     2521 gccatccgga tgcgggatgg ttgtgtcctg gatcatgtca ctcatgagat ccagggtgag
     2581 ctgatcattg gggataccta ggagcgcctg tgcgccggag ccaacgaatt cgtatctcca
     2641 gtccttgtag cgaagccgat agggaacccc cgaagccttt tcgatggcgg ttcggtagat
     2701 ctcatcggaa tagcgcagag cctcttccgc ccgtttcagc gagcagagtg ctcccagggt
     2761 ggatgcgagt aaccgaagta tctctgactg ctgaggaaac ggacgcacat ggctggatac
     2821 attgtcggtg cggattattc cgaacgttct ctctccgtcg tagatggagg ctgcagcaca
     2881 gtgtatcgga gcggatttgt cttccttctc gtcgaatcgg atgtctgtga ccaggggctg
     2941 atttccaaac accagggcgg ccaggaagtg atccttggga acggtgactc tcatccctcg
     3001 ctcgtcctga acgtggccgc tgaaatctgt cccgaacgta ccgagaatct caccaggatt
     3061 ctccggagaa cacagccaga tgccaagacg atcgaaaccg agtcgagtca gtcccagttc
     3121 gactgtgcgt cggtagaggt cgtcaatgg
//
LOCUS       DAOQUC010000315         3149 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_35709_length_3149_cov_3.169037, whole genome shotgun sequence.
ACCESSION   DAOQUC010000315 DAOQUC010000000
VERSION     DAOQUC010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3149
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35709_length_3149_cov_3.169037"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /locus_tag="PKH07_06655"
     CDS             complement(<1..226)
                     /locus_tag="PKH07_06655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34658.1"
                     /translation="MRIIDINSFIGHWFSRPVRGTAAQRLKQMDKVGIQYSFISPLEA
                     FLYDDPNEANEELFRVTEKHKDRFEPVPVVA"
     gene            complement(213..1025)
                     /locus_tag="PKH07_06660"
     CDS             complement(213..1025)
                     /locus_tag="PKH07_06660"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNT34659.1"
                     /translation="MKQLRNNGLIIDSHVHLRGELIPPDEYASWCRQYSEALGASYKV
                     ALIPFCEKWSYYPNHDEVQQGHEILAQYLESYPDLFYGWAYVNPADPDFALQEIERWV
                     ATGHFIGIKLWVAKKCSESVVVPILQRAAQLRVPVLQHAWDKSTGQLKDESLSMDVAL
                     AAQSVPNCSIIMAHLGGFWQRALKAVSPYRNIHVDCSGGWAHAGAIEKAVTLLGSERV
                     LYGSDAPLRDIPTQMSKVFGANIAASDKRKILSENFLRLTAHARRISPNANH"
     gene            complement(1047..2267)
                     /locus_tag="PKH07_06665"
     CDS             complement(1047..2267)
                     /locus_tag="PKH07_06665"
                     /inference="COORDINATES: protein motif:HMM:NF015039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HNT34660.1"
                     /translation="MKNSSLVCLCVGLILSGCVGFQKPSDVRDRGVFVMERNEYEARL
                     RSQVGQTPEQSLRQFRVDLSGVSVPDSTRVFQTVWHNPPVSQDLTGSCWAFGSTSFFE
                     SEVYRLFGQKTKISEMYTVYWEYVEKARRYVQQKGNSVFGRGSQPNATIRIWKEYGAV
                     PAESYPGRPSESTIQDHARMVSAMQDYLATVQKRKLWDEEEVVAAIRVILDKYMGKPP
                     SEVLVNGQRMSPKDYLSKVLKLDLDAYVNVMSLMQQPFFEQVEYEVADNWWHSSDYSN
                     IPVDLFIKTIRSAVNKGFSVCIAGDTSEAGLIPSMNVALIPTFDITSRFIDDAARQFR
                     FSNSSTTDDHVVHLIGHKGLGNWYLMKDSGTSARNGKCDGYMFYQEDYIKLKMMNFLV
                     HKSILEEVLAQSRP"
     gene            2523..>3149
                     /locus_tag="PKH07_06670"
     CDS             2523..>3149
                     /locus_tag="PKH07_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HNT34661.1"
                     /translation="MRELASGNQAIARGAWEAGVVLATAYPGTPSTEILEEIVKYPEI
                     DASWSPNEKVALEVGMGGAFAGARVLVAMKHVGVNVAADPLMTLSYTGLKGGLVLISA
                     DDPGMHSSQNEQDNRYYARFAKIPMLEPSDSEEARQFVKLAFEISERFDTPVMLRCTT
                     RVNHGKSIINLEERVMPECPIAFERNQQKYVMIPAYARKRHEVVESRYQ"
BASE COUNT          766 a          778 c          827 g          778 t
ORIGIN      
        1 gcgctacaac tggaacgggt tcaaatcggt ctttgtgctt ttccgtcacg cggaagagtt
       61 cttcgttagc ttcgttgggg tcgtcataga ggaaggcctc cagaggtgag atgaaactgt
      121 attggatacc gaccttgtcc atctgcttca gccgctgcgc tgcagtaccc cgaacaggtc
      181 ttgagaacca gtgtccgatg aatgagttga tatcaatgat tcgcattggg ggaaatcctc
      241 cgcgcgtggg ctgtaagtct caggaaattc tctgagagga ttttgcgctt gtcagatgct
      301 gcgatgttgg ctccaaaaac ctttgacatc tgggttggta tatcgcgcag tggagcgtct
      361 gaaccgtaaa gaaccctctc ggaacccagc agtgtgactg ccttttcaat tgcgcccgca
      421 tgcgcccatc cccctgaaca gtccacatgg atgtttcgat agggactcac ggccttgagc
      481 gcccgttgcc agaatccgcc gagatgagcc atgatgattg agcagttcgg aacgctttgg
      541 gcagccagcg ccacatccat tgaaagcgac tcatccttca actgaccggt tgatttgtcc
      601 caggcgtgtt gaagcacagg gacgcgcaac tgggctgctc tttggagaat cggaacaacg
      661 acagattcgg agcatttctt tgcgacccaa agcttgatgc cgatgaagtg cccggttgcc
      721 acccaccgtt cgatctcctg aagagcaaaa tccggatcag ccggattcac ataagcccat
      781 ccgtagaaga gatcaggata ggattccaga tactgagcga gaatctcatg tccttgctga
      841 acttcatcgt gattgggata gtaactccac ttctcgcaga acgggatgag tgccaccttg
      901 tacgaagcac ccagtgcttc agaatactgt ctgcaccagg aagcatactc atccggcgga
      961 atcagttctc cacgcaaatg aacatggctg tcaatgatca atccgttgtt tctcagttgt
     1021 ttcattgtca tcttacgaat ggaaaatcag ggacgagatt gcgccagaac ctcttccaga
     1081 atgctcttgt gaacgagaaa attcatcatt ttgagcttga tatagtcttc ctggtagaac
     1141 atatagccat cgcacttgcc gtttcgcgcc gaagtgcctg agtctttcat cagataccag
     1201 tttcccagtc ctttgtggcc tatcaaatgt accacgtggt cgtcggtcgt gctggagttg
     1261 ctgaatcgga attgcctcgc agcgtcgtca atgaatcggg aagtgatgtc aaatgttggg
     1321 atcagtgcaa cattcatgct cgggatgagc cccgcttcgg aggtgtcgcc ggcgatacag
     1381 acagagaaac ctttgttgac agcgcttctt atcgtcttga tgaagagatc aactgggatg
     1441 ttgctgtagt cactgctgtg ccaccagttg tcggcaacct catattcaac ctgctcaaag
     1501 aaaggttgtt gcataaggga catcacattg acgtaggcgt ccaggtccaa cttgagaacc
     1561 ttcgagagat agtccttggg agacattctc tggccgttga ccagaacctc cgaaggcggc
     1621 ttgcccatat acttgtccaa gatgacccgg atcgccgcga cgacttcttc ttcgtcccag
     1681 agtttgcgtt tttgaactgt tgcaaggtag tcctgcatcg cagagaccat tcgggcatga
     1741 tcttgaatcg tgctctctga aggtctccca ggataggact ccgctggaac cgctccatat
     1801 tccttccaga ttcggatagt tgcgttgggt tgagaaccac gcccaaagac agagttcccc
     1861 ttctgctgga catagcgtct tgccttttcg acatactccc agtagacggt atacatctct
     1921 gagatctttg ttttctggcc gaacagacga tagacctcgg attcaaagaa agaggttgat
     1981 ccgaacgccc aacaactgcc cgtcaagtcc tgagatactg gtgggttgtg ccagactgtc
     2041 tggaagacac gtgttgaatc gggaacactt acacccgaca agtcaaccct gaactgtcgg
     2101 agggattgct ccggggtttg accaacctgg gaacgcaaac gcgcttcata ctcgtttcgc
     2161 tccatcacaa aaacacccct gtcgcggaca tccgatggtt tttggaaacc gacacacccg
     2221 ctcagaatga gacccacaca caggcagacg agggaggaat tcttcatcca agtgctccaa
     2281 agaacagcaa aattaggttg aatcatgatt tggagagtat acgctttcag gaacgattcg
     2341 caagcagatg cccgatattc ttgacggttg tgtgtggcct atggtatttt ccgaaaactc
     2401 aacgtgtggc ggtgaataaa ataacggcca caaagtcaag agtaagtagt gctcgttgca
     2461 tcatagcatt gcctttggtt tgagcgatgg gtgcactcat ttttcctaaa ggatggagat
     2521 tcatgcggga acttgcttca ggaaatcagg cgatagcacg tggggcgtgg gaagccggag
     2581 tcgttctggc cacggcttac ccgggaactc ccagcacgga gatactggaa gaaattgtca
     2641 agtacccaga gatagacgcc tcctggtcgc ccaatgagaa ggtggctctg gaagttggca
     2701 tgggtggagc gtttgcgggt gctcgtgtgc tggtggcgat gaaacacgtt ggtgtcaatg
     2761 ttgccgcaga tcctctgatg acgttgtcct ataccggcct taagggtggg ttggttctta
     2821 tcagtgcgga tgatcctgga atgcacagct cacagaatga gcaggacaac cggtactacg
     2881 ctcgttttgc caagattcca atgcttgaac caagtgacag tgaagaagca aggcagttcg
     2941 tgaaactcgc ctttgaaatc agcgaacgtt ttgacacacc ggttatgttg cgctgtacaa
     3001 ctcgtgtcaa tcatggcaag tccatcatca acttggaaga gcgtgtgatg ccggaatgcc
     3061 caattgcttt tgagcggaat caacagaagt acgtcatgat tcccgcctat gctcgaaagc
     3121 gccatgaagt ggtggaatcc agatatcag
//
LOCUS       DAOQUC010000316         3146 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_35757_length_3146_cov_3.011970, whole genome shotgun sequence.
ACCESSION   DAOQUC010000316 DAOQUC010000000
VERSION     DAOQUC010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3146
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35757_length_3146_cov_3.011970"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1196)
                     /locus_tag="PKH07_06675"
     CDS             complement(<1..1196)
                     /locus_tag="PKH07_06675"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HNT34662.1"
                     /translation="MVVRPNKLKNLQERHLQGILQYVTLMQGVTKIQLSNLLGMSLSS
                     MNELVNRLLEQGWLRQAGTFPGDRGRKAQLFQMNPDHACLLGIDCGRKTTKAVVADLW
                     LRPIAIETTQTPFKESQQTILRNIVQTAQAALRSSGKPLKKIVGGGLGLAGQVDERAG
                     RCLNFFSKPEWNDFPIQSYLSEHLNLPFLIVGWSRGGLLAEKWFGKARNVQSLLFVHI
                     GSTISMGMAFNGSLYLSASGVSGELGHMTAKPNGPRCYCGNQGCLEAVASENGILEQV
                     ASALSEGIQSRVNDYGRKDNYTYEDVVQAANDGDKLAFQVLEQTGEYLGTCVADAINL
                     LSPELVILGGHVFDKARQIIRTVEVTIRKRALETATRNARIEFTSFGAETLVLGAAAA
                     VFECIVT"
     gene            1537..>3146
                     /locus_tag="PKH07_06680"
     CDS             1537..>3146
                     /locus_tag="PKH07_06680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34663.1"
                     /translation="MPRKTVQKRISAPSVPVEMRFPLVRSASRNPRTLVDRAKRTQGE
                     TIASGTLSFRSGKASVQLKERKAESLATALEKAAAFLLKKSKGSFVGSVLQNALKKQT
                     SFPLSVTWQNSSLMASRLEKSKMGIVVDADVNSEFKRDSDSLAIVLSMGLLSHFSWML
                     GSTEREAIHSFLGLFQLLSDKERARLVQLLRESLWDSSGCFSIFLHSACDAVLAASED
                     EAPIDLFDGVDSESLRQLGLSLKRHVQEGSVNFSPSVVRILQKVQANIAAKPALESKW
                     SNIAAAISKKSFGSAEQAELLTAAERSLKWRDKWVTWMLGRLRLDIPYNQWEIIDLLS
                     TDMEDVDEKRALVNQALSRSFDWQVEGANIQRISDWALENDVHLVGGRLSRAFGNQAH
                     LLSAARYVTDRSFLKEPVERLGRLSAGIDSLKTHTTRISSLVSRAQEVPYAQLKGAID
                     QFEQTLIDHQNDAQKEAVAHRNSAAESIFHEPVAGAKQTLTEMNAGFSEIYGQIDKVR
                     SALAQAPKRYAAYVIMLQRLHPAETINLA"
BASE COUNT          745 a          810 c          816 g          775 t
ORIGIN      
        1 gtgacaatgc attcgaacac ggccgcggcg gcgcccaaca ccagcgtctc cgctccaaag
       61 gatgtgaact cgattcgagc gtttcgagtt gcggtctcca aggcacgctt tcttattgtc
      121 acttcgacag tgcggatgat ctgtcgtgct ttgtcgaaca catgtccccc caggatcacc
      181 agttccggcg acagcagatt gatggcatct gcaacacagg tgcctaggta ctctccagtt
      241 tgttccagaa cctggaatgc gagtttatcg ccatcattgg cagcctgtac cacatcctca
      301 taggtgtagt tgtctttgcg tccgtagtcg ttcacacgag actgaatgcc ctcactcagt
      361 gctgaagcca cctgttcgag gatgccgttc tctgaagcaa cggcttcaag gcagccctgg
      421 ttgccgcagt agcagcgagg cccgttgggt ttggccgtca tgtgacccag ttctccggaa
      481 acgcctgaag cactcaggta gaggcttccg ttgaaagcca tgcccatgct gatcgtgctt
      541 ccgatgtgga cgaacagcag gctctgaaca ttcctggcct tcccaaacca tttctcagcc
      601 aacagtccac cacgtgacca tccgacgatc aagaagggca gattgagatg ctcgctcagg
      661 tatgactgta ttgggaaatc attccattcg ggttttgaga agaagttcag gcatctgccg
      721 gctcgttcat caacctgtcc tgcaagcccc aagcctcccc cgacgatctt cttcagaggc
      781 tttcctgagg agcgaagtgc cgcctgagcc gtttgcacga tattgcgaag aatcgtctgc
      841 tgactttcct tgaagggagt ctgagtcgtc tctattgcaa taggtctcag ccagagatct
      901 gcaacgaccg ctttggttgt ctttcggccg cagtcaatac cgagcagaca ggcatgatcg
      961 ggattcatct ggaagagttg ggcttttcga ccgcgatccc ctgggaaagt tcctgcttgt
     1021 ctgagccagc cctgttcgag caaccggttg acaagttcgt tcatggatga caggctcatc
     1081 ccaagcaaat ttgaaagctg aatcttggtc acgccctgca tgagcgttac atattgtaga
     1141 atcccttgga gatgccgctc ctgcaggttt ttcaacttgt tggggcgaac gaccatggta
     1201 tactccgttt taactgaatc ctcggcttgt ttggaatcct tcaacgccat tcggattcga
     1261 acactgagga atataaccaa aaatgcagga ttagtcaaga aatgagagag aataagacaa
     1321 atcgaagagg atttattata taaatcaata aatatgaata agttatttaa ttcagctata
     1381 ggcataattg ttcgaataga tcgacattgc catgcttatt ggcaagattc ttcttgacat
     1441 aatggttttt ttgcccgatt ctcgcattca tattttgcat tcttagtaaa aataagggag
     1501 aaccaagttg agagtctccc agtaaggaag atagcaatgc caaggaagac tgttcagaag
     1561 agaatctccg ctccgtctgt gcccgtagag atgcgatttc cactcgtgcg atcagcatcc
     1621 aggaatcctc gcaccctcgt ggatcgcgcc aagcgcaccc aaggcgaaac cattgcctct
     1681 ggaactttgt cctttcgaag tggaaaggca agcgtccaac tgaaggagcg caaagccgaa
     1741 tcgttggcca ctgcgcttga aaaagcggcc gcttttctgc tgaagaaatc caagggttcc
     1801 tttgtcggca gcgttctgca gaatgccctc aagaagcaga catcgtttcc actttctgtg
     1861 acgtggcaga actcctcgct catggcttcc cgtctcgaga agtccaagat ggggatcgtg
     1921 gttgatgccg atgtgaactc cgagttcaag agagactcgg actctctggc tattgttctc
     1981 tccatggggc tcttgagtca tttctcgtgg atgctcggca gcacagagcg agaggccata
     2041 cacagttttc tgggcctgtt ccagttgctg tcagacaagg agcgagcgcg actggtgcaa
     2101 cttcttcgag aaagcctttg ggacagcagc ggatgttttt ccatctttct tcactccgca
     2161 tgtgatgcgg tgcttgcagc gtcggaagac gaggcaccca tagatctgtt tgacggggtg
     2221 gattccgaga gtctcaggca acttggtctt tcactgaaac gtcatgtgca agagggtagt
     2281 gtgaatttct ccccctcggt tgtcagaatt cttcagaagg ttcaagccaa cattgcggca
     2341 aaacctgctc ttgaatcgaa atggtcgaat atcgccgcag ccatttcaaa aaagtctttt
     2401 ggttctgcgg agcaggcgga actgctgacc gcagccgagc gctctctcaa atggcgtgac
     2461 aaatgggtga catggatgtt ggggcgtctg cggctggata ttccttacaa ccagtgggag
     2521 atcatcgatc tcttgtctac cgatatggaa gatgttgacg agaagcgcgc tttggtgaat
     2581 caggcgctct cgcgatcttt cgactggcag gtggaaggtg caaacattca acgcatcagc
     2641 gactgggctc tggaaaatga tgtccatctt gttggtggac gtctgagccg cgcctttgga
     2701 aaccaggctc atctgctttc cgcagcccgg tatgtaaccg accgttcttt tctgaaggaa
     2761 cctgttgaaa gactcggcag gctttcggcg ggcattgatt ccctcaagac tcatacgact
     2821 cgcatctcca gtctggtcag cagagcacaa gaggtgcctt acgcccagtt gaagggagcc
     2881 atcgatcagt tcgaacaaac gctcatagac catcagaacg atgctcagaa ggaagccgtt
     2941 gctcacagaa actccgctgc cgagagcatc tttcatgaac ccgttgcagg tgccaagcag
     3001 actctgacgg agatgaatgc gggtttctcg gaaatctacg gccagataga caaggttcga
     3061 agtgccctgg cacaagcccc gaaaagatac gccgcctacg tcatcatgct tcagcggctg
     3121 catccggcgg agacgatcaa cctggc
//
LOCUS       DAOQUC010000317         3138 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_35890_length_3138_cov_3.599416, whole genome shotgun sequence.
ACCESSION   DAOQUC010000317 DAOQUC010000000
VERSION     DAOQUC010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3138
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35890_length_3138_cov_3.599416"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1172)
                     /locus_tag="PKH07_06685"
     CDS             complement(<1..1172)
                     /locus_tag="PKH07_06685"
                     /inference="COORDINATES: protein motif:HMM:NF018004.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HNT34664.1"
                     /translation="MSEVLPFQGIRFDVPRIGGDMWPVLSPPWDKITPKIGETLRSAH
                     PNNMAHLVVPAAGEVETPWPASARLLFDKWLQSGVINRDPGTGFYGCRMTFTHQGTRY
                     CRSGFMGLCRLGRPEVSKVHPHERIFPDHYQTRLALIRETRTNFEPVFLIYPDPKEET
                     ISLLGEPDRVSGIRAEDPDQNIVYEFWPVFCRDRCERIQQLMAARDLLIADGHHRYGA
                     AQAYAEEWHANNPTAPAVTGVDYRMCYLTPLESDGVLILPTHRVFRGLLEHLPALESS
                     MMARYFQVVSDQYGNEKQLLDAMWAHREREPHAKVLGLFDGTRFVLLRWTGAEPDSYS
                     TTHAERTSVWKSLDVNVLHEILLLGVLKWNRGTDSQWVSHHRDAGTAVAEVRAAPG"
     gene            complement(1169..1774)
                     /locus_tag="PKH07_06690"
     CDS             complement(1169..1774)
                     /locus_tag="PKH07_06690"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNT34665.1"
                     /translation="MSPTKKHAPSQQESVRALLKTHQAEDEHSEQVARLALVLFDQLK
                     DLHRLGERWRNLLYLGALLHDIGLSESISRHHKHGCEIIANAPLDGFSEAEKWILANL
                     ARYHRKALPKASHECFSKLCAEDQEGVQKTAALLRVADGLDRSHRNAVSSIECQIQTG
                     AVLASLEGRDLDDEVWAAEKKRDLFEEVYGMRLIFSRKESS"
     gene            complement(1970..2947)
                     /locus_tag="PKH07_06695"
     CDS             complement(1970..2947)
                     /locus_tag="PKH07_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020381680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HNT34666.1"
                     /translation="MSGQRNFVIGVDLGGTNIAAGLVNSAGQVLRQEKVKTKAQKGAQ
                     EILDRLMSTIEKVSDKDTWKQVQAIGIGSPGPLDPETGVILDTPNLSLKNCPLGPAVQ
                     VRFGVPVFVDNDVNVGTLGEYVYGAGRGFRNVVGIFVGTGIGGGLILDGKLWHGTSKN
                     AGEIGHMVIQPDGPLCGCGNRGCLEALASRKSIMRDLALAVQNGRPTSLLDSVGKDRD
                     LLKVRSKAIAKSYHNGDPLVKETLDKAAGYIGLAVKSLVHLLGAELFVLGGGVVEAIG
                     EAFLEPIRAEVLHCLPGTAEGVEVRISALGDQAGILGAAALARTRLTES"
     gene            complement(2952..>3138)
                     /locus_tag="PKH07_06700"
     CDS             complement(2952..>3138)
                     /locus_tag="PKH07_06700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HNT34667.1"
                     /translation="IGLDPDPATSFKIFQARVIEEFNNMIREFGFFVVDGEKPIHTQQ
                     RIIRDYFKKFLAPTESS"
BASE COUNT          712 a          939 c          783 g          704 t
ORIGIN      
        1 ctcccaggag cggctcgtac ttcggcgaca gccgtgcccg catcccgatg atgggagacc
       61 cattgagagt ccgtccctcg gttccatttc aacacgccca gcaatagtat ctcatgcagc
      121 acgttgacgt ccagagattt ccagacggaa gtcctctctg cgtgggtggt ggagtaggaa
      181 tccggctcgg cacccgtcca tcgcaagagg acaaaacgcg tcccgtcaaa tagtcccagc
      241 accttggcgt ggggttcccg ttcccggtga gcccacatgg catccagcag ttgcttttca
      301 tttccatact ggtctgaaac aacctggaaa tatcgtgcca tcatactgct ttcaagtgcc
      361 gggagatgct caagcaggcc gcgaaagaca cggtgtgtcg gcagaatcag gaccccgtca
      421 ctttccagcg gtgtcaaata gcacatgcgg tagtccacac ccgtgaccgc gggtgctgtt
      481 gggttgttcg catgccactc ttcggcatac gcctgcgcag caccgtatcg gtgatgtccg
      541 tcggcaatca ggagatctcg cgctgccatc agttgctgaa tcctctcgca gcgatctctg
      601 caaaaaaccg gccagaattc atagacgata ttctgatcgg gatcttcggc acgaatcccc
      661 gaaacccgat caggctcccc cagaagagaa attgtctcct ctttcggatc cggatagatc
      721 aaaaacaccg gctcgaagtt cgtacgcgtt tcacgaatga gtgccagtct ggtctgatag
      781 tgatccggga aaatcctctc atgaggatga accttggaga cttccggacg tcccagtcgg
      841 cacagtccca taaaacctga acggcagtat cgagtcccct gatgcgtgaa tgtcatgcga
      901 catccgtaga atcccgttcc cggatctcga ttgatgaccc cgctctggag ccatttgtcg
      961 aaaagcagtc gagcggacgc aggccagggc gtttcgactt ccccggccgc aggcaccacc
     1021 agatgcgcca tgttgttggg atgtgctgac cgaagcgtct ctccaatctt cggtgtaatc
     1081 ttgtcccaag gcggtgaaag tacaggccac atatctccgc caatcctggg aacatcaaaa
     1141 cgaatccctt gaaaaggtaa tacttcgctc atgaactctc ttttctcgaa aaaatgaggc
     1201 gcatcccgta gacttcttca aagagatcgc gctttttttc tgcagcccag acttcgtcat
     1261 ccaagtcgcg gccttccaaa ctggccagga cggctcctgt ctgaatctga cactcgatgc
     1321 tacttacagc atttcgatgg gatcgatcaa gtccgtcggc gacgcgcaac aaggccgcgg
     1381 ttttctgaac accttcctga tcctcggcac agagtttact gaaacactcg tgactggctt
     1441 tgggcaaggc ctttcgatgg taccgcgcaa ggttggccag gatccatttc tccgcttcgg
     1501 agaagccgtc gagtggcgcg ttggcgatta tctcgcaccc gtgtttgtgg tgccgactga
     1561 tactttccga cagaccaatg tcatgcagaa gcgccccaag ataaagaaga ttcctccagc
     1621 gttctcccaa ccgatggaga tctttcaact gatcgaaaag caccaacgcg agtcgggcaa
     1681 cctgctcgga atgctcatcc tcggcctgat gcgttttgag cagggcacga acactttctt
     1741 gctgtgaggg agcgtgtttc tttgttggag acaagaacgt ttgtccttct ttcctatata
     1801 ctctctcgaa accccccctg ccccccttaa caaagggggg taagaacggg tctgcccccc
     1861 ttaacaaggg gggatcaagg ggggatttgt catccccttc gggtgcgctt agcccataag
     1921 aggcttcgat tgaagaactc ttggctggag attgtcccga gaaagcagat catgactcgg
     1981 tgagcctcgt tcttgccaga gccgcagcgc cgaggatgcc cgcctgatct cccaaggccg
     2041 agattcgaac ttccacgcct tctgccgtac ctggaagaca gtggagaacc tccgcccgaa
     2101 tgggctccag gaaagcctcc ccaatggctt caaccacacc cccccccaaa acaaacaact
     2161 ccgcccccag caggtgaaca agactcttga cggccaaccc gatgtagccc gccgctttgt
     2221 ccagcgtctc cttcaccaaa ggatctccgt tgtggtacga ctttgcaatg gccttgctgc
     2281 gaaccttgag caaatcccta tccttgccaa ccgagtccaa cagtgatgtc ggacgtccat
     2341 tctgaaccgc caaagccaga tcacgcatga tggatttccg actggccagt gcctccagac
     2401 agccccggtt gccgcatcca cacaagggac catccggttg aatgaccatg tggccgatct
     2461 caccggcatt cttgctggtg ccgtgccaga gcttcccgtc aagaatcagt ccgccaccga
     2521 tgcccgtccc gacaaaaatg cccacgacat tccgaaatcc gcgacctgct ccgtagacat
     2581 attctcccaa ggttccaacg ttgacgtcgt tgtccacaaa gacaggcacg ccaaaacgta
     2641 cctgaacagc aggtcccagc ggacagttct tcagggagag atttggggta tccaggatga
     2701 ctccggtctc cggatccaaa gggccgggag agccaatgcc tatcgcctgc acttgtttcc
     2761 aggtgtcttt gtcagaaacc ttctcaatgg tgctcatcag tcgatcaaga atctcctggg
     2821 cacctttctg agccttggtt ttgacttttt cctgacgcag aacctggccg gcggaattga
     2881 caagtcccgc ggcaatgttg gttcccccca agtcaacccc gatgacgaaa tttctctgtc
     2941 cactcatggg cttaggatga ctctgtcggt gcaagaaact tcttgaaata gtcccgaata
     3001 atcctctgtt gtgtatggat gggcttctcg ccatctacaa caaagaatcc aaactcacgg
     3061 atcatattgt tgaactcttc gatgacgcgc gcctggaata tcttgaagga cgtcgccgga
     3121 tccggatcca gaccgatg
//
LOCUS       DAOQUC010000318         3128 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_36045_length_3128_cov_3.888057, whole genome shotgun sequence.
ACCESSION   DAOQUC010000318 DAOQUC010000000
VERSION     DAOQUC010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3128
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36045_length_3128_cov_3.888057"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(25..1149)
                     /locus_tag="PKH07_06705"
     CDS             complement(25..1149)
                     /locus_tag="PKH07_06705"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HNT34668.1"
                     /translation="MKEYKSILLVRLRAVGEVVMTLPVLDIVRRNYPNAFLAMLVESP
                     CEEIFEADSRADKVFVFNKPYLRSLPYHKKMKAFWEFVFSLRQQRFDLAVDLHNVSRS
                     QWLTFLSGARERIGMRTRALTYHLHTTTMEALRDTRQHNIARYLQILRNMGMNVDEYT
                     YEMKISPDALQWAGQWLTEQTRNRAVRVVLHPGAAHVHKCWPVEQFARFGDLLAERHQ
                     ADIIITCGPGEMSLGEQVQKNMRAESRLVADVGLQQLAALLSKCDLLVSNDTGPTHIS
                     SVVGTPTIAVIGPTNPYVSGPATTCSRIVWAERYCSPCYRDMFTCPIRKCFMMIEPEQ
                     VLEIANKILQEGRPIQHESAVPGIHPRKDLPKRAYVASRV"
     gene            complement(1146..2123)
                     /locus_tag="PKH07_06710"
     CDS             complement(1146..2123)
                     /locus_tag="PKH07_06710"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HNT34669.1"
                     /translation="MEILRKYGRGILISLVFGAVVFIVLSFWSGFGEVFEELKQFNWW
                     LVPLVVALAFANYLVRFVKFHYYVRVLDIDLSKKDSFAIFLSGLVMSVTPGKFGEVLK
                     SVLIKAVNGTSISRSAPIVLAERFTDFVALALMSLLGVYYYNFGAKTYIISAILILGP
                     MFLVSSKSASHGLIRVLGKLPGLSKASPKMLVAYESTASLLSMKNLFSMTIISILSWF
                     CECFAFYLVCNGFSGSGVPLLGLTFVYSFSILVGAIMMLPGGIGATEASLTGLMVTAM
                     GMVKSVAVAATFIVRACTIWFAVLIGAATLMAYQKKLSPKAVQEIAEGQ"
     gene            complement(2214..>3128)
                     /locus_tag="PKH07_06715"
     CDS             complement(2214..>3128)
                     /locus_tag="PKH07_06715"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNT34670.1"
                     /translation="RSGVEHVSGVRKAVIPAAGFGTRLYPATKAVKKELFPIVDRDGL
                     AKPFIQLIVQEAFSAGIERVCLVLQPDDVPIFQSYFQGDLSPDLSEKLSVRSWAREQT
                     EVLRQMGGRIDYAVQKTQDGFGHAVLCAREWVGEEPFLLLLGDHAYISDIEKPCALQL
                     IETFQRSGTSCVGVDRTQGEFVHLYGTLKAQPDLRQSRQYVVERYVEKPTLEYANANL
                     EVQGLGENTFLCLFGQYVLKSEIFDFISHLLKDGVKNQGEVQFTDALQMLRDSEGCHH
                     ALEIEGRRYDIGVPEQYVATLMALAGKH"
BASE COUNT          785 a          850 c          790 g          703 t
ORIGIN      
        1 ttctcaggca ctgctgaacc tctttcacac acgacttgcg acatatgccc gcttgggcaa
       61 gtccttgcgt ggatgaattc ctggaacggc actttcgtgt tgtatgggac gtccctcttg
      121 aagaatcttg ttagcgatct ccagaacctg ttccggctcg atcatcatga agcatttgcg
      181 tatcggacag gtgaacatgt cccgatagca gggcgagcag tagcgttccg cccacacgat
      241 cctgctgcat gtcgtggcag gaccgctgac gtaaggattc gttggaccta tgaccgcgat
      301 ggttggagtc ccgacaacag aggaaatgtg ggttggtccg gtgtcattgg agaccaggag
      361 atcgcacttg gagagaagcg cagccagctg ttgaagaccg acatctgcaa caaggcgcga
      421 ctcggctctc atgttcttct ggacttgctc tcccaatgac atctcgcctg gcccacaggt
      481 gatgatgata tccgcctggt gcctttctgc aaggagatca ccgaagcgtg cgaactgctc
      541 aacaggccag cacttgtgaa catgggctgc gcccggatga agcaccaccc gaacggcgcg
      601 gtttcgagtt tgttcagtca accattgtcc tgcccattgg agcgcatctg gggatatctt
      661 catctcatag gtgtactcat ccacgttcat gcccatgttc ctgaggattt ggagatatcg
      721 ggcaatgttg tgttggcggg tgtcccgaag ggcttccatg gttgtcgtgt gcagatgata
      781 ggtcagagcg cgtgttcgca taccgatgcg ttcccgtgct ccggaaagga aagtcaacca
      841 ttggcttctg gagacgttat gcagatcgac ggcgagatcg aaccgctgct gtctcagcga
      901 aaatacaaac tcccaaaacg ccttcatctt cttgtggtag ggaaggctcc gaaggtaagg
      961 tttgttgaac acaaagacct tatctgcccg gctgtctgct tcaaagattt cctcgcaggg
     1021 tgattcaacg agcattgcaa gaaaagcgtt cggatagttg cgacggacaa tatccagaac
     1081 cgggagagtc ataacaactt ccccgacggc acgtaaccga acaagcagta tggatttgta
     1141 ctccttcatt gtccttctgc tatctcttgc accgctttgg ggctgagttt cttctgatag
     1201 gccatcagcg ttgctgctcc gatcagtacg gcaaaccaga tggtacatgc ccgaacaatg
     1261 aatgttgccg ccactgcaac agacttaacc attcccatag cggtcaccat tagcccagtc
     1321 agactcgctt ccgtggctcc aatgccaccc ggcagcatca tgatggcacc gacgagaatc
     1381 gagaaagaat agacaaaagt caaccccaac agaggaacgc ctgaacccga gaatccgttg
     1441 cagaccagat agaaggcgaa gcactcgcag aaccaggaga gaatcgagat aatcgtcatc
     1501 gagaacaggt tcttcatgga caaaagtgag gcggtgctct cgtaggcaac gagcatcttg
     1561 ggtgaggcct ttgagagtcc aggcagtttc cccagaactc ggatcaaccc atgcgatgct
     1621 gacttggagc tgaccagaaa cattggacct agaatcaaga tggcactgat gatgtacgtc
     1681 ttggctccaa aattgtagta gtagacgccc aagagcgaca tcaacgccaa agccacaaaa
     1741 tcggtaaacc gttccgcaag gacgatgggt gcggaacgac tgatggacgt gccattgacg
     1801 gccttgatga gaacggattt cagcacttca ccgaacttac caggtgttac gctcatcacg
     1861 agtccggaaa ggaagattgc gaaactgtcc ttcttgctga gatcaatgtc caggacgcga
     1921 acgtagtagt gaaatttgac aaaccgaacg agatagtttg cgaaggccaa ggcgaccacg
     1981 agcggaacca gccaccagtt gaactgcttc agttcctcaa agacttcgcc gaagccagac
     2041 cagaacgaga gcacgatgaa aacaactgcc ccaaacacca gggaaatcag aatgccgcgg
     2101 ccgtattttc gaagtatctc cacgagttct tcaccttgtc cttgctcgtc cccaaatctt
     2161 tcggccttca gactccttgg aatatccgcc gaaagatttc ctgcgaggcg cttctagtgt
     2221 ttacctgcca aagccatcaa tgtggcaaca tactgctccg ggacgccaat gtcatagcgt
     2281 ctcccctcta tctccaaagc atgatggcag ccctcagaat cccgaagcat ctgcaaggca
     2341 tctgtgaact gcacttcccc ttgattcttg actccatcct tcaatagatg gctgatgaag
     2401 tcgaaaattt cggatttcaa aacgtattgt ccgaagagac agagaaacgt gttctcgccc
     2461 agaccttgca cttcgagatt tgcattggca tattccaacg ttggcttctc aacgtaacgt
     2521 tcgacgacgt actgcctgct ctggcgcaag tccggttgag ccttgagcgt tccgtacagg
     2581 tgcacaaatt ccccttgggt gcgatccacg ccaacacaac tcgttccact tctctgaaac
     2641 gtttcgatca actgaagagc gcatggtttt tcaatgtctg agatatatgc gtggtctccc
     2701 aacagcagaa gaaaaggctc ctcacctacc cactcacggg cgcacagcac cgcatgacca
     2761 aagccatcct gcgttttttg aacagcatag tcaatccgac cgcccatctg tcggagcacc
     2821 tctgtctgtt ctcttgccca ggaacgcaca gagagctttt cggaaagatc gggggaaaga
     2881 tcgccctgaa agtaactctg aaaaatgggc acatcatcag gttgaagaac aagacagaca
     2941 cgctcaatgc ccgccgaaaa ggcttcctga acgatcaact ggatgaacgg ctttgccagc
     3001 ccatctcgat cgacgatcgg aaagagttcc ttcttgaccg ccttggttgc cggatagaga
     3061 cgggttccga agcccgctgc ggggatgact gctttgcgaa cgccgctcac gtgttcgact
     3121 cctgacct
//
LOCUS       DAOQUC010000319         3112 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_36305_length_3112_cov_2.946680, whole genome shotgun sequence.
ACCESSION   DAOQUC010000319 DAOQUC010000000
VERSION     DAOQUC010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3112
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36305_length_3112_cov_2.946680"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..436)
                     /locus_tag="PKH07_06720"
     CDS             complement(<1..436)
                     /locus_tag="PKH07_06720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34671.1"
                     /translation="MRLRRSEIPQLNRFFEGLLWCLAVAALLTLLFQYGFILTLSQQR
                     LILYVDIAIVLAFVSEAFVKLLLSSSRKRFFRKRLADFILIFLFPILYFFIGRKSAIP
                     IKMLILLSQLYLLGAIGLRALRLNKVIVRWRVSPAKLVVYSFV"
     gene            740..>3112
                     /locus_tag="PKH07_06725"
     CDS             740..>3112
                     /locus_tag="PKH07_06725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNT34672.1"
                     /translation="MNETIEILRTHESWLMKRILGYAKDRGYAKYTSTLEEAWRLSIA
                     GLTASLAEGARRFDCIPELGPEEDLTKDPLTSFGVVEARRHRERGISLPMFLGLMKYY
                     RQSYCDLITEQVASEKLPRVRLFLDRCFDRIEIAFCQEWAKCDSDERVRELQKNARLI
                     MNEKTAYLTVFESLAEPVILLDQDDLIVNMNHAAVSLVIPEPTPGALYYHPEEILRRE
                     GTNPQNSCHAFIGRKVHQVLPWIFGGETEFPSKSQLWRAQGIVANQQRCYDVKHTEML
                     DLSGKFTTGILNLNDVTDRENAANILRQSEEKFRTYFEHSRVGMAITSPDYGWLEVNQ
                     RLCELLRCFPKDLGSMTLETLTHPDDRHKDRERTEKMLRGEIDGYTVEKRLVCMDGGY
                     VDTITSVRAIRRSDGTVSHCFVQVQDVSNLKQIERRLRESEQMLRLVLDNVPQRVFWK
                     NREFKYLGCNRPFARDAHLESPQEITGKEDFDLSWKEVAHLYRRDDQEVMESGQPKLG
                     YEEPQSRPDGSLMWLLTSKVPLRNEQGDVVGVLGTYEDITMKKSVESALRISEGRLRS
                     LFDAITESTLLVDIKGTILDLNEAAARVAGLSREQAQGTNLSEHIPPEVAALHVPIIL
                     ECYEKAEPVQFELVLHKRILSVSVYPVQDDSKRVVQFAIFAQDCTERHQAEQVRERLA
                     LELARKNKELQQLIFIASHDLRSPLVNVMGFSRELESHIQDLSKAIEATCADAVRQGS
                     LARLLTQDVPECLSFIAASTAQMDRLLKSLLKISRLGQSVLHFENLDMRVF"
BASE COUNT          793 a          753 c          862 g          704 t
ORIGIN      
        1 gaacgaaact gtagacgact aattttgctg gcgagacgcg ccatcgaaca atgaccttgt
       61 tgagacgaag cgctcgaaga ccaatggctc cgagcagata gagctgacta agaagaatga
      121 gcatcttgat cggaatggcc gactttcggc caatgaagaa gtagaggatc ggaaacaaaa
      181 aaatcaggat aaaatccgca agtcttttcc tgaagaagcg tttccgactg ctcgaaagca
      241 ggagtttgac aaaggcctcc gacacaaaag caaggactat cgcgatatcc acatacagaa
      301 tcaggcgctg ttgagagagg gtcaatatga aaccgtactg aaaaagaagc gtcaacaatg
      361 ccgcaacggc gagacaccac aggagaccct caaagaaccg atttaattgg ggtatctctg
      421 aacgacgtag tctcaagata tactctcagt ccaacttctt gactgctgca tccagatggg
      481 aatccggatg acacgtctct tttggtgtgg gcacatccca tcccgaacta gtcaacgctc
      541 taaccttcag taagagtatc agaagggcgg ttcaaaggca agacttccca agaggcgaca
      601 aatgatcgca cttgctttgt tttgggatgc gggtaagaat ctatctggct attgcgagtg
      661 ctcgtgtgtt ttcttgctgg gttggttgag cgccaggaaa cgatgtctcg cggagtctgc
      721 gctggtggag atggcaggga tgaatgagac aatcgagatt ctcagaaccc acgagtcttg
      781 gctcatgaaa aggatccttg gctacgccaa ggatcgggga tacgccaagt atacatctac
      841 gttggaggag gcgtggcggc tttccattgc cggtctgacc gcctctctgg ctgagggtgc
      901 gagacgattt gactgcattc ctgaacttgg ccctgaagag gatttgacaa aggatccttt
      961 gacttccttt ggtgttgtcg aagccagacg acaccgtgaa cgtgggatct ccctgccgat
     1021 gtttctcggt ctgatgaagt actaccgtca aagctattgt gatctcatca ccgagcaagt
     1081 tgcctcagag aaactgcctc gggttcgcct gtttctggat cggtgttttg atcggatcga
     1141 gatcgctttc tgtcaggagt gggcgaagtg tgacagtgat gaacgtgttc gggagttgca
     1201 gaagaatgct cgactgatca tgaatgagaa gacggcttac ctgacagtct tcgaaagcct
     1261 cgccgagccg gtgattttgc tcgatcagga cgatctgatt gtgaacatga accatgcagc
     1321 ggtatcattg gtcattcccg aaccgacacc cggggcactc tactaccatc ccgaggagat
     1381 tcttcgcaga gaaggcacga accctcagaa cagttgccat gctttcatcg gcagaaaggt
     1441 acaccaggtt ctcccctgga ttttcggggg tgaaacggag tttccctcca agtcacaatt
     1501 gtggagagcg caaggaatcg ttgccaacca gcaacgctgt tatgatgtca agcacaccga
     1561 gatgcttgat ctgagcggca aattcactac cggaatcctg aacctcaacg atgtgaccga
     1621 tcgagagaat gccgccaaca tactgcgaca aagcgaggag aaattcagaa cctacttcga
     1681 gcactctcgt gttggaatgg caatcacgtc accggactat gggtggctcg aagtcaacca
     1741 gcggttgtgt gagttgttgc gatgttttcc caaggacctg ggttcgatga cgctggagac
     1801 actgacgcac cctgatgaca ggcacaagga tcgggagcgt actgagaaga tgctccgtgg
     1861 tgaaattgat gggtataccg tcgagaaaag actcgtatgt atggacggtg gctatgtgga
     1921 caccatcact tcagtgcgtg cgattcgacg ctctgatgga acggtctcgc actgctttgt
     1981 tcaagttcaa gacgtcagca atctcaagca gatcgagcga aggctccgag agtcggaaca
     2041 aatgctgcga ctggtactgg acaatgtgcc ccagcgcgta ttctggaaga atcgagagtt
     2101 taagtacctt ggctgcaacc gaccttttgc gcgagatgca catctggagt ctccacaaga
     2161 aatcactggg aaagaggact tcgatctgtc ctggaaggag gtagcacacc tctaccgccg
     2221 tgatgaccaa gaggtcatgg agtcaggaca gccgaaactg ggatacgagg agccacagtc
     2281 caggccggac ggaagtctca tgtggcttct caccagcaaa gttccccttc gaaacgaaca
     2341 aggagatgtt gtcggggttc tgggaaccta cgaagacatc acgatgaaga agagcgtcga
     2401 gtccgccctt cgaatcagcg aggggcgact ccgctccctt ttcgatgcaa ttaccgaaag
     2461 cacgcttctg gtggacatca aagggactat tctggatctc aacgaagcgg ctgcacgggt
     2521 tgccggtctg tcccgagaac aggctcaagg aaccaatctc tccgaacaca tacctcccga
     2581 agtggcagcg ctccatgtcc caataatact tgagtgctat gagaaagcgg agccagtaca
     2641 gtttgaactt gttctgcata agaggattct ctcggtctct gtgtatcccg ttcaggatga
     2701 ctccaagcgc gtagtgcagt ttgccatctt cgcccaagac tgtaccgagc gccaccaggc
     2761 agagcaggtg cgtgagcgtc tggctttgga gctggcaaga aagaacaagg agctgcagca
     2821 gttgattttt atcgcatccc atgatctgcg atcgcctctg gtcaacgtca tggggttttc
     2881 cagggagttg gagtcgcaca ttcaggatct gtccaaggcg attgaagcaa cctgtgccga
     2941 tgcggtcaga cagggaagtc ttgcccgtct gttgacgcag gatgttcctg agtgcctcag
     3001 ctttattgct gcttcgacgg ctcagatgga cagactactc aagagccttt tgaagatctc
     3061 acgtctgggt caaagcgtgc tgcactttga gaatctggat atgcgggtct tt
//
LOCUS       DAOQUC010000320         3111 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_36323_length_3111_cov_3.055301, whole genome shotgun sequence.
ACCESSION   DAOQUC010000320 DAOQUC010000000
VERSION     DAOQUC010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3111
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36323_length_3111_cov_3.055301"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(469..1671)
                     /locus_tag="PKH07_06730"
     CDS             complement(469..1671)
                     /locus_tag="PKH07_06730"
                     /inference="COORDINATES: protein motif:HMM:NF015316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HNT34673.1"
                     /translation="MRESSFFKTFRKSLSSPYRVAIVLFVLLAGSEAALGQIPGMDAE
                     FAGAGARSMAMGSAFIGLADDATASEFNPAGLQRLRRPELALQFRLVDERRTEHRFAK
                     SLREVFEGNGQPRSGTFHDTYEDISFAAFTYPMQRLTLSLSQMSPIYFERTYFDRATV
                     WGQDIVFGRAQDVSLRNYGLSAAYSLTDTLSLGVTGKFGRFTYDEKNSWGTDDVLRDN
                     TLGANFGVLWKFHPWWSLGAVYKTSQHVEGSFEGTRIKLRIPETWGTGLAFHPNDRWR
                     FLADVDYINWSNYDDSLAVNRNNVTGKLEWKHQDVTRYHLGSEYLLGHFWNTGVFLRA
                     GYMLEDSNARYYAGSDPYFLKAAPKPDDIDHYSVGLGFARQRFQLDFACDYSEEKTQV
                     IASTVIYF"
     gene            complement(1690..>3111)
                     /locus_tag="PKH07_06735"
     CDS             complement(1690..>3111)
                     /locus_tag="PKH07_06735"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HNT34674.1"
                     /translation="VDQQPERSGWRVEDGKWTALDVRQMAESKALLPLIVFANACQSG
                     RTEDWGKEEHLYGLARAYLLAGVQHYIGTLWDILDVPGSEFAAVFYDSLLEGQTIGTA
                     IREARRKMSQSHGEKSLIWASYVLYGDPTAQVFGIPDLPEATAPSQTLATAPVTEQDH
                     PKPAPLSVGGETRSQQQAPSKWKALVGVGVVIAILLGLWAALRDAPAPKPPTSRPVGA
                     TQTLLDELSSRTRDPEYQKRLQDQDDWTSRRLSVALLPLSSESDSADAVAVAEMLRAG
                     LEQQFGTGRGVQVLERRQLESILAEQNLGASSLADPETLARLGRLRFARLLLSGKVFA
                     DPSTGTFLSYSLFDCETTETVATGMVGGASDPADITRAVAERSFTNLAEKYPIRGRVL
                     QIEDNRLFLNIGSDVGVQKEQSFRILSDPQQRGTQMVDLMQEMAQGTIEEVAPGASIG
                     VLQSDGTSLHEGIRVEIKERRRI"
BASE COUNT          736 a          886 c          787 g          702 t
ORIGIN      
        1 gcctgtggct tccggatcta cgcgtaggac acgagcttct gctgctgcag tgaagagagc
       61 catggctacg cttgccaaaa cgaaaagact gaaacactct gtcagcaagg gagtcactgc
      121 tctggatcga aatgcctgcc tggatccaaa tccgtgacga agtgatttcc tgagcatgca
      181 tgttcccgtc tgaccggata ttccacatct acctgacttt gcagaagcat tcataagcag
      241 cattgatcca agactccctg attttgtcgt actcgtcgtt ctgaaagccc caatggcctg
      301 tcgctgaaag ccactcttca ggaatgttcc aaccattcca tacaattgtc aagacagacg
      361 gatcaagtat ctcctgacag atcggtctct cagcgacacc gagtccaaaa gagccttgtc
      421 atccaccgag caagcatcct ggcgagtggg cataacctga aagtcagact agaagtagat
      481 gacagttgaa gcgatgacct gcgttttttc ctcactgtag tcacaggcaa agtccagctg
      541 aaatcgctgt cttgcgaatc ctagtccgac ggagtagtga tcaatgtcat ccggcttggg
      601 cgctgccttc aggaaatagg gatccgagcc tgcgtagtat cttgcattgg agtcttcgag
      661 catgtagcct gcacgcagaa agacccccgt gttccagaag tgtccgagaa gatactcgct
      721 tcccagatgg tagcgcgtca catcctgatg cttccattca agtttccctg tgacattgtt
      781 ccggttgact gcaagtgagt catcatagtt tgaccagttg atatagtcca catctgccag
      841 aaagcgccat cggtcattgg gatgaaacgc gagtccggtg ccccacgttt ctggtatccg
      901 aagtttgatt cgagtgcctt cgaaagatcc ctcaacgtgt tgggatgtct tatacacggc
      961 acccaatgac caccagggat ggaatttcca gagaacgccg aaattggcac cgagcgtatt
     1021 gtcgcgaagg acatcatccg tgccccagct gttcttttca tcgtaggtga atcttccgaa
     1081 ttttccagtt actccaagcg agagtgtgtc tgtgagtgag taggcggcgg aaagaccata
     1141 gttgcgcaat gagacatcct gggcacgtcc aaacacgatg tcctgtcccc agacggttgc
     1201 ccgatcaaag taagttcgtt cgaaatagat gggactcatc tgagagagag acagggtgag
     1261 acgttgcatg ggataggtaa aggcggcgaa agagatgtct tcataggtat cgtgaaatgt
     1321 tccacttcgc ggctgtccat ttccctcaaa gacttctctc agcgacttgg caaaccgatg
     1381 ctccgtccgc cgctcatcaa ccaaccgaaa ctgcagcgcc aactcaggac ggcgaagacg
     1441 ctgcaaaccc gccgggttga actcggacgc cgtcgcatca tccgccaaac caatgaaggc
     1501 agatcccatc gccatggatc gtgctcctgc accagcaaat tctgcatcca tgcctggaat
     1561 ctgacccaac gcagcttcgc tgccagccag tagaacgaac aggacgattg ccacacggta
     1621 cggtgatgac aaagatttcc gaaacgtctt gaagaaagat gactctctca tggaactact
     1681 cctgagaaac tagatgcgac gccgctcttt gatctcaacc cgaatcccct catgtaagga
     1741 ggtgccatcg gactgaagaa caccgatcga agctcctgga gcaacctctt ctattgttcc
     1801 ctgcgccatc tcctgcatca gatctaccat ctgagttcca cgctgctgag gatcgctcag
     1861 gattcgaaac gactgctcct tctgaacacc aacgtccgac ccgatattca ggaaaagacg
     1921 gttgtcttct atttgaagaa ctcggccgcg aatgggatat ttctccgcaa gatttgtgaa
     1981 ggaacgttca gcgacagctc ttgtgatatc tgcaggatcg ctggctccgc cgaccatgcc
     2041 ggtggcgact gtttcggttg tttcgcagtc aaagagactg taggagagga atgttcccgt
     2101 actcgggtcg gcaaagactt ttcctgacag gagaagtcga gcaaagcgga ggcgtcccaa
     2161 acgcgccagt gtttcgggat ccgccaacga agaagcgcca agattctgtt cagcgagaat
     2221 gctctccaac tgccgacgct cgagaacctg cactccacgc cccgtgccaa actgttgctc
     2281 cagccccgcg cgcagcatct ccgcgacagc cacagcgtca gccgaatcgc tctcagacga
     2341 caaaggcaac agagcgacgg agagtcttcg agaagtccag tcgtcttgat cctgaagccg
     2401 cttctgatat tcaggatcac gcgttcggct gctgagctca tccaataacg tttgtgttgc
     2461 accaacgggt cttgacgttg gtggtttggg ggcaggcgcg tctcgaagcg ctgcccagag
     2521 accgagcaga atggcgatca caactcccac gcccacaagt gccttccatt ttgaaggcgc
     2581 ttgttgctga ctcctggtct cccctccgac actcaaggga gcaggctttg ggtggtcctg
     2641 ctctgtcaca ggcgcagttg ccaaggtctg acttggtgct gtcgcttccg gaagatccgg
     2701 tatgccaaag acctgagcgg taggatcccc atagaggaca taacttgccc agatgagtga
     2761 cttctcccca tgtgactgcg acatttttct ccgcgcttca cgtattgctg ttccgatggt
     2821 ttgcccttcc agcagcgagt catagaatac agcagcaaac tcactgccag gcacgtcgag
     2881 aatgtcccag agcgttccaa tgtagtgctg cactcctgca agcagatagg cgcgcgccag
     2941 tccatacagg tgctcttcct tcccccagtc ctcggttcta cccgattggc aggcattggc
     3001 gaacacgatc agcggaagca gcgctttgct ctccgccatc tgacgcacat caagcgcagt
     3061 ccactttcca tcctcaactc gccacccgct gcgttccggc tgctggtcaa c
//
LOCUS       DAOQUC010000321         3105 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_36426_length_3105_cov_4.251803, whole genome shotgun sequence.
ACCESSION   DAOQUC010000321 DAOQUC010000000
VERSION     DAOQUC010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3105
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36426_length_3105_cov_4.251803"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1019
                     /locus_tag="PKH07_06740"
     CDS             <1..1019
                     /locus_tag="PKH07_06740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="type III restriction endonuclease subunit R"
                     /protein_id="HNT34675.1"
                     /translation="EYTKLMGMSKPTPPLHITVRTDLSKEQQEQLDIRLPFLSPRIHR
                     AKGNIQSLDVGSIQPGNRKIKDFSKQKKREIVFTDILTDEEDHTTELETILEPIFASV
                     VGYIARKIVLDVRVLGHFDCICSKMREYIETRLFEEPVCADDENILRNLCESVVRKFL
                     IDEFKKALNALTVKNADTVHVTGEIRVSQAMPQVVSYQDHLFPTKSLLTPLIGDSDLE
                     LEFAKSLDCYKDIVSFAKNRPLGVNFYMEYQTQNGSIARYYPDFIVKKSDEEIWIIET
                     KGLETTEAPGKFKRLLQWCQDATEYDQNGKNQYNALYVVEDEWEEYRPSSFAELCELF
                     RPGE"
     gene            1634..>3105
                     /locus_tag="PKH07_06745"
     CDS             1634..>3105
                     /locus_tag="PKH07_06745"
                     /inference="COORDINATES: protein motif:HMM:NF024573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNT34676.1"
                     /translation="MLLKQLVVKGFKSFADKTEVGIPPGITIFVGPNGCGKSNVSDAI
                     RWVMGEQNPRNLRGMKMQDMIFAGAETRKAESMSEVSLVFDNPDGFFNLPQAEVMITR
                     RLYRSGESEYLINKTPVRLKDILELFMDTGLGHHGYTLIEQGRVEHMLSSKPSDRLAI
                     FEEAAGVTRYKARRDEALRKLHRAEQDLLRLNDIEEELGKQSRSLRRQAQQASRHKRL
                     VEELQSLDQIIASREGTRLTAEKQALEKQTQEVTDLLNGEQSQIVALETRQAEVSLHI
                     DGFTARLREQHENYHRLQMDIQKRESQLAFLKETKTELSQRRANLEKEIQQNQSKKQH
                     LTAEIENKRLQIEMLEQQQNEHQQQYDSRLKNLEQIRADRRSLDQQMEGLRNRLWEIQ
                     KDILSCHNEMQQIQQQKEFLDSQDERALRERESLEQQLQNVIESQGHLSEEIQAKREE
                     LQQTIERLTEVQESLARSSDAIKDLTERLRQEEKLLDQYKV"
BASE COUNT          845 a          764 c          836 g          660 t
ORIGIN      
        1 tcgagtacac aaagctcatg ggcatgagca aacccacccc gccgcttcat attacggtca
       61 gaactgattt gagcaaagag caacaggaac agctcgatat tcgtctcccg tttttgtcgc
      121 cccgcattca cagagccaag gggaatatcc agtctttgga cgtcgggagc attcaacctg
      181 ggaatcggaa gatcaaagac ttctcaaagc agaaaaaacg ggaaatcgtc ttcacggaca
      241 tccttacaga cgaagaagat cacacgacgg aactcgagac cattctggag ccgatttttg
      301 ccagcgttgt cggttacatt gcgcgaaaga tcgtccttga tgtccgtgtg ttgggtcatt
      361 tcgactgtat ctgctcgaag atgcgcgaat acatcgaaac acggctgttt gaggaacctg
      421 tttgtgcgga cgatgaaaac attctgagaa acctttgcga aagcgtcgtt cgcaaatttc
      481 tgattgatga gttcaagaag gcgctcaacg ctttgacagt caagaatgcg gacaccgtgc
      541 atgtgactgg ggagattcgc gtgagccagg caatgccgca ggtggtcagt tatcaagacc
      601 acctctttcc aacgaagtca ttgctcaccc cgcttatcgg agacagcgat ttagaactgg
      661 agttcgccaa atcactcgac tgctacaagg acattgtgtc gttcgcaaag aaccgcccgt
      721 tgggagtgaa cttctatatg gagtaccaaa cgcagaacgg ttccatcgcc cgttactatc
      781 ccgatttcat cgtcaagaag agcgatgagg aaatatggat tatcgaaacc aaggggttgg
      841 agacaacgga agcgccaggg aaattcaagc gtttgcttca gtggtgtcag gacgccacgg
      901 aatacgatca aaatgggaag aaccagtaca acgcccttta cgttgtggaa gacgagtggg
      961 aagagtatag accttccagt tttgcggaat tgtgtgagtt gttcagaccc ggagagtagg
     1021 atcttgtttg tagttacgcc gtgaggcgtg atgaggatat gcccttacgg gcaaactaca
     1081 aacttcctcg ttcgtagtta cgccgtgagg cgtgatcctc tcccgtttgt agttaccccg
     1141 tcaggggtga tccggatatg ccctcacggg cacactacaa acagatgatc ccgagtcgag
     1201 ccgaaaggga actgtggctc gggacatcca cgctcacagt catgtccgct cctgttccat
     1261 gagggttttt tgagtctctt tcgtgccaac ccttccaaag atcaggactt gcgtattccc
     1321 ggacagacca caatatctag tatgaggggg atcaaaaata tgctttctat tgtggattga
     1381 ccttgactta gcagaacgta cgccttatat tctcactaac actgacaggg gataactgag
     1441 agctccgctt gacacgaaaa cagcaaaggc gtgtccggtt ctcaggagca gatcggcgtt
     1501 cgcggacaga gtgacttttg ggtctgcttg ccttggtcag gaattccggt tccgtcttcc
     1561 ctttgtggga gaggctcacg agccggagca gatgtgtgta actgtgcaag agttttgtcc
     1621 agggagttaa gtcatgcttt tgaagcaact cgtcgtgaag ggttttaagt cttttgcgga
     1681 caagacggaa gtcgggattc caccggggat tacgattttt gtgggtccga acggatgcgg
     1741 aaagagcaat gtctcagacg ccatccgctg ggtgatgggt gagcagaatc cgcgcaacct
     1801 tcgtggtatg aagatgcagg acatgatctt cgcgggtgcg gaaactcgaa aagccgaatc
     1861 catgtcggaa gtctcattgg tgttcgacaa tcccgatgga ttcttcaatc tgccacaggc
     1921 tgaggtgatg ataaccagac gcctgtaccg ttcgggtgaa tccgagtatc tgatcaacaa
     1981 gacgccggtc cggctcaaag atatcctcga actcttcatg gacaccggct tgggacatca
     2041 cggctacacc ctgatcgagc agggtcgcgt ggaacacatg ctcagttcca aaccttccga
     2101 ccgacttgcc atcttcgaag aagcggcagg tgtcactcgg tacaaagccc gaagggacga
     2161 agctcttcga aaactgcacc gtgccgaaca ggacttgctt cggctgaacg acatcgaaga
     2221 ggaacttggc aagcagtccc gttccttgcg aagacaggct cagcaggcat ctcgacacaa
     2281 gcggcttgtg gaggaattgc agtcgctgga tcagattatt gcttctcggg aaggcacacg
     2341 actcacagcc gaaaaacaag cactcgaaaa gcagacgcaa gaagtgaccg atctcctgaa
     2401 cggagagcag tcacagatcg tggcgctgga aacccggcag gcagaagtct cgttgcacat
     2461 tgacggattc accgcgcgcc tgcgggaaca gcacgagaac taccaccggc tccagatgga
     2521 catccaaaaa cgcgaaagtc agctggcatt tctcaaggag accaagacgg agctctccca
     2581 gcgacgtgcc aatctggaaa aggaaatcca gcagaaccag tctaagaagc agcatcttac
     2641 tgccgaaatc gagaacaaac gacttcagat agaaatgctc gaacagcaac agaacgaaca
     2701 ccaacagcag tatgactcac ggctgaagaa tctcgaacaa atccgggcag accggcgaag
     2761 cctggatcaa cagatggaag gtcttcggaa tcggctctgg gagattcaaa aggacattct
     2821 ttcctgccac aacgaaatgc agcagatcca acagcagaag gaatttctgg attcccagga
     2881 tgagcgtgca ttgcgtgagc gggaatccct tgaacagcaa cttcagaatg taatcgaatc
     2941 gcaaggccat ttgtcagaag agatacaggc caagagagaa gaactgcaac agaccatcga
     3001 gcgcctgacc gaagttcagg agagtcttgc gcgtagcagc gacgctatca aggatctcac
     3061 cgaacgactt cggcaggagg agaagctgct ggatcaatac aaggt
//
LOCUS       DAOQUC010000322         3105 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_36429_length_3105_cov_3.552131, whole genome shotgun sequence.
ACCESSION   DAOQUC010000322 DAOQUC010000000
VERSION     DAOQUC010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3105
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36429_length_3105_cov_3.552131"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(47..1885)
                     /locus_tag="PKH07_06750"
     CDS             complement(47..1885)
                     /locus_tag="PKH07_06750"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNT34677.1"
                     /translation="MDGELRLTPIRVLALRAHDASGGVRKTGPIEREKGKVFSNKGWR
                     DLFDQRTTLFLSAVSAVSVVALTVGMIVFAFEYGRMDHFDRESFLSLKASFREDPSSE
                     ALANAIRVQDVFIREQHLRYIRRVKYGGLLLLGSMIALALCLRRLASLREQMPRARRA
                     AQIESEQEQYRFKVITMLLVVILPVLLILFVAIHRPPKLDLSVTTSGLGSAVLVASSD
                     EPVSDDQKWPQLFGFMGTRRIPDMDVPTNWDAATGENLLWKADIPIGGNSSPVVWGNK
                     AFVTGADLQSRHVYCFDTRDGSLVWTCRIQTDALLGADFRLPAETGLASPTVATDGTL
                     VYAFFGTNELAAVDFNGQMVWSRWFGKPESIYGIATSPIVHDGKIILQLDQGDADENI
                     SFLYALNTSDGKELWKTPRDNGATWTSPVISESDGQTVIVTTSDPYVIGYDLGSGREL
                     WRVKALSGDVAPVPTVGGGMVFVTTQYAPLSAIRLGGSGDVTETHVAWSYDGDTPDVS
                     SPLTDGLLLILANGYGTVVCFDAVTGEEIWKEEFRQGFWSSPVLVGDKVFLTDMDGKT
                     FIFMMERSYVQKGVGSVGEFIGATPAFADSRIFIRSQRTLYCLGKK"
     gene            complement(1767..3026)
                     /locus_tag="PKH07_06755"
     CDS             complement(1767..3026)
                     /locus_tag="PKH07_06755"
                     /inference="COORDINATES: protein motif:HMM:NF024585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNT34678.1"
                     /translation="MSARQARSYVSLYKVLEPCAGGSPHPESEKRSAMAGSVSWFPQS
                     GKLKFAVRLMFLWVLLLALPDGTGAQQNFPPPEFESGYNLPVATFQLFHAGAMPYLDV
                     LLLVLALAAASWFLHKIRSRKGVLILGLVCLAYFGFYRKGCICPVGSVQNMFLGVLDS
                     SFPVSIPVALVFLTPLVFALVFGRVFCGAVCPLGMVQDIVIFKPLRVPLWIEKPLSLL
                     RFVYLGLALFLVLSSRRFIICEFDPFVGFFRMNGFAWKQILGGLLLLLGLFVARPYCR
                     YLCPYGALLSLPSRASNVKVRITPDSCIRCGLCEPKCPFGAIRPPQEESVASTRIQRL
                     SIVASIVVPVGLGGVGYYLLNRSVAGALLGLWVGFVVSVRLMELIYFGRRTTFDADPS
                     SCLACARCFRWCPKDRAYREREGKSVQ"
BASE COUNT          860 a          884 c          770 g          591 t
ORIGIN      
        1 agaccgtcca gatcacgaac gtctttctct tccatcactt cagacatcac ttctttccaa
       61 ggcagtacaa cgtgcgttgg cttcggatga agattcttga atctgcaaac gcaggagtcg
      121 ccccgatgaa ctctccgacg cttccaacgc ctttctgaac ataggatcgc tccatcatga
      181 agatgaacgt tttgccatcc atgtcagtca gaaacacctt gtctccgacc aagacgggag
      241 aagaccagaa accctgtcgg aattcctctt tccagatctc ctcaccggtc acagcgtcga
      301 aacacaccac agttccatat ccgttggcaa ggatcagcaa cagaccatcc gtcagcggac
      361 ttgacacatc aggagtgtca ccatcgtagc tccaggcaac atgcgtctca gtcacatcgc
      421 cagagccacc aaggcggatc gcactcagcg gcgcatactg ggttgtcacg aagaccatac
      481 caccaccgac tgtgggaact ggtgctacat ctccagagag ggccttgacg cgccagagtt
      541 cccttcccga cccaagatcg tagccgatca cataggggtc agaagtggtg acaatgactg
      601 tctgcccatc agactcagag atcactggac ttgtccacgt cgccccattg tccctcggtg
      661 ttttccagag ctcctttcca tcagaggtgt tgagagcgta gagaaatgat atgttctcgt
      721 cggcatctcc ctggtcgagc tgaagaatta tcttgccgtc gtgaacaatc ggtgaagtcg
      781 ctattccata gatgctttca ggctttccaa accagcgaga ccagaccatc tgcccgttga
      841 agtcaactgc agccagctcg ttggtgccaa agaacgcata gaccaaggtg ccgtctgtcg
      901 caacagttgg cgatgcaagt cccgtctccg ccggaagacg gaagtccgct cccagcaatg
      961 cgtcagtctg aatcctgcac gtccatacca aagaaccgtc gcgggtatca aaacagtaga
     1021 cgtggcgcga ttggagatcg gctccggtga caaaagcctt gttaccccaa acaactggtg
     1081 agctgttccc accaatggga atgtccgcct tccagagtaa gttctctcct gtggctgcat
     1141 cccagtttgt tggaacatcc atatcaggaa ttctccgagt ccccataaac ccaaacagtt
     1201 ggggccattt ctggtcgtcc gaaaccggtt catccgaaga ggccacgagc actgctgatc
     1261 ccaagccaga agtcgtcaca gacagatcta acttcggagg gcgatgaatc gcgacgaaca
     1321 ggataagcaa gacgggcagg ataacaacaa gcagcatcgt gatgaccttg aagcggtatt
     1381 gttcctgctc actctcgatc tgtgcagcgc gtcgcgcgcg aggcatctgc tctcgaagac
     1441 tcgcgagtcg tctcaagcag agtgccagag cgatcatgct gcccagaagg agcagtccac
     1501 catacttcac tctgcgaatg tagcggaggt gttgctcccg aataaaaaca tcctgcacgc
     1561 ggatggcgtt cgcaagagct tcactgctcg ggtcttcacg aaacgacgcc ttcagagaaa
     1621 gaaaactctc gcggtcaaag tgatccattc tgccgtattc aaaggcgaag acgatcattc
     1681 cgactgtcag cgcaacgaca gagacagcgc tcacagcgct aagaaacagc gttgtcctct
     1741 ggtcaaacaa gtcacgccag cccttgttac tgaacacttt tcccttctct ctctcgatag
     1801 gcccggtctt tcggacacca cctgaagcat cgtgcgcacg caaggcaaga actcggatcg
     1861 gcgtcaaacg tagttcgccg tccaaagtaa atgagttcca ttagccggac cgacacaaca
     1921 aacccgaccc agagaccaag cagagcgccg gcaacactgc gattgagcag gtaataaccc
     1981 actcctccca gtccaactgg cacaactatc gaagcgacga tggaaagtct ctggatcctc
     2041 gttgaagcaa ctgactcttc ctgtgggggt ctgatggcac caaaagggca tttgggctca
     2101 caaagcccac accgaataca tgaatccggc gtgatcctga ccttgacgtt actggctcgg
     2161 gaaggcagag aaagcagagc cccgtatgga cagagataac gacaataagg acgggcaaca
     2221 aagagaccga gaagaagcag gagtccaccg agaatctgtt tccaggcaaa tccattcatc
     2281 cggaagaatc ccacgaaagg atcgaactca cagatgataa accgacgaga gctgagaaca
     2341 aggaataaag ccaatcccag gtatacaaac cgcagaaggc tcaacggctt ctcgatccaa
     2401 agaggaacac gaagtggctt gaagataact atgtcctgaa ccatcccgag agggcaaacc
     2461 gctccacaaa acactctgcc gaacacaagc gcaaaaacga gtggcgtcaa aaagacgagg
     2521 gccactggga tggaaacagg gaacgaggag tcgaggactc cgagaaacat gttctgcact
     2581 gaaccaacgg gacagataca tcccttcctg tagaatccga aatacgcaag acagacaaga
     2641 ccaagaatca ggacaccctt tctgcttcgg atcttgtgaa gaaaccagct cgctgcagcg
     2701 agagcaagga cgagaagcaa gacatcgaga tacggcatcg cgccagcgtg aaagagctga
     2761 aaagtcgcaa cgggtaggtt gtacccagac tcaaactctg gtggagggaa attctgttgc
     2821 gctccagtgc catcaggcaa cgcaagcagg agcacccaga ggaacataag ccgaacggca
     2881 aatttgagct taccggactg cggaaaccaa gagacagatc cagccatagc gcttctcttc
     2941 tcggattctg gatgcggcga accgcctgca caaggctcga gcaccttgta cagtgaaacg
     3001 tatgagcgag cctgacgggc actcatcaat ttctcccgac tgccatgtct tcaaaagcga
     3061 acgatctcac gcagtgcttt gagaaaggcc tgctgacgca ctcca
//
LOCUS       DAOQUC010000323         3103 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_36469_length_3103_cov_4.225722, whole genome shotgun sequence.
ACCESSION   DAOQUC010000323 DAOQUC010000000
VERSION     DAOQUC010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3103
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36469_length_3103_cov_4.225722"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(247..1746)
                     /locus_tag="PKH07_06760"
     CDS             complement(247..1746)
                     /locus_tag="PKH07_06760"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HNT34679.1"
                     /translation="MRKGTRHTTKTDPRLAALSILKGVRVGKVLVDELLQEHLSEIKD
                     PRDRALAHELAFGVLRQRSLLDWYLLQNIEGQSLRVPAVVQDILRLGAYQILFCERIP
                     TYAAVSQTVGLCRETGFSGLCGLVNGILRSLDRRTRAGVSSTTVEEDSDPETPAQSLS
                     DLPLPKEDEKPLTIQGAEGIFPEQTEGFVKHVSVRYSLPEWIIEKLLEVWSPEDVVGF
                     ATASLEVPPLTIRVHPRILSPSLFAEQCRDSGIEAEPVSWLPDAFFVRSRIPVSQLPG
                     IEQGGLQVQDLGAQLTTLLLDPLPGERILEVGSAPGGKTTHISERIRPTGRLFAVDNS
                     AHRCEVLQKNLDRIGARNVQLIIADGTKPLPLEPDSMDRALVDAPCSGLGTLRRRVDL
                     KWRLQPRDIVALSETQRALLASTAEYVKPDGVLVYSTCTILREENEAIVEEFIADHPN
                     WKVESARAFLPDPMKSAVLPSGGLQTLPHRHGMDGNFGIRLRRTPKSTK"
     gene            complement(1797..2474)
                     /locus_tag="PKH07_06765"
     CDS             complement(1797..2474)
                     /locus_tag="PKH07_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012465821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HNT34680.1"
                     /translation="MFLDPRYFVYVFPAILLVIYAQMKVKSTFAEFSRYRTRLGYTGG
                     EVARGLLDKAGLHDVPVEMSRGWLSDHYDPLKKVLRLSPEVYQSNSVASVGVAAHEVG
                     HALQDQKGYLPLRVRTGLFPLTAAGSQLGPILVILGVIFRFGGLINIGIVLFSAAVFF
                     TLITLPVEFNASRRAVAQLVQGGYVSDQEVAGVKKVLSAASLTYVAAAAQAILTLLYY
                     LSLRNRR"
     gene            complement(2525..>3103)
                     /locus_tag="PKH07_06770"
     CDS             complement(2525..>3103)
                     /locus_tag="PKH07_06770"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTAP family purine nucleoside phosphorylase"
                     /protein_id="HNT34681.1"
                     /translation="LEIKPCDFVVPDQVIDRTRQRPLTFFDDLAVHLPFADPFCPQLS
                     RLYVESATKAGVTIHEGGTYVCIEGPAFSTKAESRLYRSWEADIIGMTCLPEARLARE
                     AEMCYATLAASTDYDVWHEEPVTTEIVLANFARNLDNIRKIVREAISKVSDETECCCQ
                     QALRGTLITASSAVTDSDRERYGLLLGRYLKA"
BASE COUNT          773 a          890 c          793 g          647 t
ORIGIN      
        1 atgagtaaag cccgacagtt cccaccgccg ccactaaaac cagcaggaag gtgtagaaaa
       61 caagacgtct gagacttcga aaaaccttca ccatggtgtc cagtatctcc gattcggaat
      121 cacaaccgaa ttctatgctg cgcttgactc acggcgcaaa gcctgcgcgc acgatcgcaa
      181 ttgccccaag aatcctccca acccccctta ttaagggggg ataaggggga ttagaagcac
      241 cggtcattat ttagtagatt tcggcgttcg acgcaaacga attccgaaat ttccatccat
      301 cccgtgccga tgaggaagtg tttgaagccc acccgatggg agaaccgccg atttcatcgg
      361 gtcggggaga aaggctcggg cagattccac cttccaattc gggtgatctg ctatgaattc
      421 ctctacgatc gcctcattct cttcacgcaa aatggtacac gtgctgtaga ccaatacgcc
      481 atcgggttta acatactccg cagtggatgc cagcaacgcc cgctgtgtct cggaaagtgc
      541 gacaatatcc ctgggctgaa gtcgccattt caaatccacc cgtcggcgca acgttcccaa
      601 gccggaacag ggtgcatcca caagcgcccg atccatggaa tcgggctcca gaggaagagg
      661 ctttgtccca tcagcaatga tgagttgaac atttctcgcg ccgattcgat cgagattttt
      721 ctgaagaacc tcgcaacggt gggcactgtt gtccacagcg aataaccttc ccgttggtcg
      781 aatccgctct gatatgtgcg ttgtctttcc acctggagcg ctgcctactt caaggattct
      841 ctctcccgga agcggatcca gaagcagtgt cgtcaactgc gcaccaagat cctgaacctg
      901 aagcccacct tgctcgatgc ccggtaattg tgaaacggga attctcgacc gaacaaagaa
      961 agcatctggc agccaggaaa caggttctgc ctcgatcccg gaatcacggc attgctctgc
     1021 aaaaagggaa ggagagagaa tgcgcggatg aactcgaatg gtcagcggcg gcacttccag
     1081 tgatgcagtg gcaaacccaa caacgtcctc tggcgaccag acctcaagca gcttttcgat
     1141 gatccattcg gggagagagt atcttacgct gacgtgctta acaaaaccct ctgtctgttc
     1201 tggaaaaatc ccctctgccc cctgtatcgt gagaggcttt tcatcctcct ttggcaaggg
     1261 gagatccgag agagattgag caggcgtttc aggatcagag tcctcttcga ctgttgttga
     1321 agatactcct gcccgcgttc ttcgatccag tgacctcagt attccattga ccagtccaca
     1381 cagaccggag aagcccgttt cccgacacag cccaactgtc tgtgaaaccg cggcgtaagt
     1441 gggaatccgt tcgcagaaaa gaatctgata ggccccgagt cggagaatgt cctgaacaac
     1501 agcaggaacg cgcaaggatt ggccttcaat gttctgaagc agataccagt ccaacaaaga
     1561 acgctgtcgc agcacaccaa aggcaagctc gtgggcgaga gcacgatccc gaggatcctt
     1621 gatttcggag agatgttctt gcagaagctc gtcaacaaga accttcccga cacgaacgcc
     1681 tttcaggatg ctgagggctg cgagcctcgg atcggttttt gtggtgtggc gtgttccctt
     1741 tcgcatcgct ctccaacggg acttcttatg aaacgaaccg gttgaggccg gttagtctac
     1801 cgtctgttgc gcagggaaag atagtagagc agtgtcaaaa tcgcctgtgc cgccgcggcc
     1861 acgtatgtca gggacgcagc ggagagcacc ttcttgacac ctgccacttc ttgatcggag
     1921 acatatccac cctgaaccag ttgtgccaca gcccgacgac tggcattgaa ttcgactggc
     1981 agcgtgatca gcgtgaaaaa gaccgctgcg gagaaaagca caataccaat gttgatcaga
     2041 ccgccaaagc ggaaaatgac tcccaagata accagaatgg gccccagttg actgcctgca
     2101 gcagtcaggg gaaacaaacc ggtgcgaaca cgcagaggca gatacccttt ctgatcctgc
     2161 agcgcgtgac caacttcgtg tgccgccaca ccgacggaag cgacactgtt gctctgatac
     2221 acttcggggg aaagacgaag aactttcttc aaagggtcat agtgatcgga cagccaacca
     2281 cgactcatct cgacaggaac atcgtgcagc cctgctttgt ccagcaaacc ccgcgcgact
     2341 tctccaccgg tgtaaccgag tcgcgtccgg tatcggctga attcggcaaa ggtgctcttg
     2401 accttcatct gagcgtagat caccagcaga atcgctggaa acacataaac aaaataccgg
     2461 ggatccagga acatctttcg tcacctcgtc atgccgcctc attctccatt gccagcacgg
     2521 aaaatcaggc tttcaaatat cgtccgagca acagcccgta acgctcgcgg tcagaatccg
     2581 tcaccgcact ggacgccgtg atgagcgtcc cgcgaagcgc ttgttggcag caacactcag
     2641 tctcatcgct cactttggaa atcgcctccc ggacaatctt cctgatattg tccaggttcc
     2701 tggcgaaatt cgccaggacg atttccgtcg ttaccggctc ctcgtgccaa acatcatagt
     2761 cagtcgaagc ggcgagcgtg gcgtagcaca tctccgcctc acgagccaag cgcgcttcgg
     2821 gaaggcaggt catcccgatg atgtccgctt cccagctgcg atacagccgc gattcggctt
     2881 tggtcgagaa cgccggacct tctatgcaaa catacgttcc cccctcatgt attgttacgc
     2941 ccgcttttgt ggcgctttcg acatagagac gcgacagctg agggcaaaaa gggtcggcaa
     3001 aggggagatg aaccgcaaga tcatcaaaga acgtcagagg cctttgtcgg gtacgatcaa
     3061 tcacctggtc gggaaccacg aaatcacacg gcttgatctc cag
//
LOCUS       DAOQUC010000324         3103 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_36481_length_3103_cov_3.092192, whole genome shotgun sequence.
ACCESSION   DAOQUC010000324 DAOQUC010000000
VERSION     DAOQUC010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3103
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36481_length_3103_cov_3.092192"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(172..633)
                     /locus_tag="PKH07_06775"
     CDS             complement(172..633)
                     /locus_tag="PKH07_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012882734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HNT34682.1"
                     /translation="MKATEGRVGRVFIIRLEDGDVVPECIERFAEEKGVHHGQAVLIG
                     GLGPGQVVVGPRDSESMPPEPMLLPVDGAHEVVAVGVLAPTETGKPILHIHGALGRSG
                     QTMTGCLRPGVETWVVAEVVLYEILNAKATRIPDERTGFSLLQPGVANSEP"
     gene            804..2372
                     /locus_tag="PKH07_06780"
     CDS             804..2372
                     /locus_tag="PKH07_06780"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HNT34683.1"
                     /translation="MIYSAKHFLDKRQTGHLRRFVNETIKRWQVPGVAIGVVSDNETV
                     LAEGFGIRGLKCKSPVSAETLFAIASCTKAFTSLAVAIAVEEGKISWDTPVREYLPEF
                     RLSDPCASERITPKDLLTHASGLPRHDDVWWKCNDTREEYVHKLRFLEPSKDLRSKYQ
                     YQNLMYMVAGYLVGKVYGTTWEAFLQSRLLDPLKMTATNFSIADSRKSDDYAFPHVTI
                     KNKTRSTDFYDILEGVGPAGSMNSNVTDMLKWLSFHLNKGLIDGQQIVPEACVLKTHT
                     PQIVCEPSGFKAMPYLTYAMGWHATQYRGFRLLFHGGNVGGFSCNTAFLPDENVGIVV
                     LTNLSSSSVGKIVTYHLCDLLLLGNQTSNKTVSSKSTHRKGARIADTSAGTGLLEMKP
                     LPWNDLYQEQVDKERAEAKKKQQQQRRNRVKGTKPSHSLAEFVGNYRNQAYGSMRVTL
                     QKGSLYCEYQEKVLPLVHYHYDVFERVDASYGSRTLVAFHTRTNGHIGNLSIRLEPEV
                     SEIIFERVRDGSPV"
     gene            complement(2411..2836)
                     /locus_tag="PKH07_06785"
     CDS             complement(2411..2836)
                     /locus_tag="PKH07_06785"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNT34684.1"
                     /translation="MQYVYKTENLKRYRFPTHINDIVIDRSESRCSEVFVVVVEPHKG
                     THLHKHEDTEQVFYVLSGNGRLEIGQEKAPHTIEPGDVIRVPLSTWHSVHCVGERDLV
                     YLAIDCFDGGRPNAEPTWDAHVRVMCQQQGWEYDKVVEK"
BASE COUNT          749 a          834 c          801 g          719 t
ORIGIN      
        1 cctgaaaggt atatccggtt ttgttgacgg cgggattgca gaacagattg gtaatgctcc
       61 ccgcaatcgt aacaggcact cgatagggtc tgccttcgtg aaaagcagcc cagaccttgc
      121 ggacttcttc attgtgcttc tcaaagtcaa tcggctctgt gaggaagaaa ctcatggttc
      181 gctgtttgcc actccgggtt gcaggagact gaacccagtc ctttcatcgg gaattcttgt
      241 cgccttggcg ttgagtatct cgtacagaac aacctcagca acgacccagg tttccacccc
      301 gggcctcaga caaccggtca tggtctgccc tgagcgtccg agagcaccat gaatgtgcag
      361 gatcggcttg cccgtttccg tgggagccag cacgccgacc gcaacgacct catgggcacc
      421 gtccaccggc agcaacatcg gttcgggcgg catagactcg gaatcgcgcg gcccgacaac
      481 aacctgaccc ggaccgagac caccaatcaa gacggcctgg ccatggtgta cacccttttc
      541 ctccgcaaaa cgctcaatgc attccggaac aacatctcca tcctcaagtc ggatgatgaa
      601 cacccgaccg actctgcctt cggttgcttt catggccttt ccacctccgc ggtgcaataa
      661 gtcacttcaa cttctttgcg gatacagcat atcctgaatc gtcggagatt tcaaagcagg
      721 tttcggttga cgttccgacg gtgaagtgaa taatgaacca ataccgatag ggactcagtc
      781 aacagaatgg aggctccaga tttatgatct actcggcaaa gcatttcctc gacaagaggc
      841 agacagggca cctgagaagg tttgtcaatg aaacaatcaa gaggtggcag gttccgggag
      901 ttgccatcgg cgtcgtttcc gacaatgaga ccgttcttgc cgaaggcttt gggattcgcg
      961 gcttgaaatg caagtcacct gtgagtgctg agaccctttt cgcgattgca tcatgcacca
     1021 aggcattcac cagtcttgct gttgcgatcg ctgtcgagga agggaagatc agttgggata
     1081 ctccggttcg ggagtatctg cccgagttcc gtctctccga tccctgtgcc tcggagcgca
     1141 tcacgcccaa agatttgctg acgcatgcat ccggtcttcc tagacatgac gatgtgtggt
     1201 ggaaatgcaa cgataccaga gaggaatatg tccacaaact tcgatttctc gagccgagca
     1261 aagatcttcg ttccaagtat cagtatcaga acctgatgta catggtcgcc ggttacctgg
     1321 tcggcaaagt ctacggaacc acttgggagg cctttctgca gagtcggctg ctcgatcccc
     1381 ttaagatgac agccaccaac ttctcgatcg cagactccag aaagtcggat gactacgcct
     1441 tcccccatgt cacgatcaag aacaagacga gaagcaccga tttctatgac atccttgagg
     1501 gagtgggtcc tgctggaagc atgaactcca atgtaacaga tatgctcaag tggctttcgt
     1561 ttcacttgaa taaaggtctg attgatggtc agcagatcgt tccagaggcc tgcgtattga
     1621 agacccatac accacagatc gtctgcgagc catcgggatt caaagccatg ccgtacctga
     1681 cctatgccat gggctggcat gccacgcagt atcgaggctt tcgcctgttg ttccatggcg
     1741 gcaacgttgg cggtttcagc tgcaacacgg cctttctacc ggacgagaat gtggggattg
     1801 tcgtcctcac caatctgagt agttcctcgg tggggaagat cgtcacctat cacctctgtg
     1861 atctcttgct cctgggaaac cagacttcga acaagacggt ttcttccaag agtacacatc
     1921 gcaagggagc gcgaatagcc gacacatctg caggcaccgg cctgctcgaa atgaaaccgt
     1981 tgccatggaa cgatctctac caggagcagg tggataagga gagggcggag gccaagaaga
     2041 agcaacagca acagcgcagg aatcgagtga agggaacaaa gccctcacat tcactggccg
     2101 aatttgttgg gaactatcgc aatcaagcct atggatcaat gcgggtcact ctccagaaag
     2161 ggagcctgta ctgcgagtat caggagaagg ttctgccctt ggtgcattat cactatgacg
     2221 ttttcgaacg ggtggacgcc tcatacggtt ctcgaacact cgttgcattt cacaccagga
     2281 caaatggaca catcgggaat ctctcgattc gcctggagcc cgaagtatca gagatcatct
     2341 ttgagagagt ccgtgacggg tcgcctgttt agtcgctggt cgggtctgtg gagcagtcaa
     2401 aaagagatga ctatttctcc acgactttgt cgtactccca gccctgctgt tggcacatga
     2461 cgcgaacgtg cgcatcccag gttggttcgg cattgggacg tccaccatca aaacagtcta
     2521 tcgccaggta gacgagatct ctctccccga cacagtggac gctgtgccaa gtagacagcg
     2581 ggacacggat cacgtcaccc ggctcaatgg tgtgcggagc cttttcctga ccgatctcca
     2641 aacggccatt accactgagt acatagaaga cctgttccgt gtcttcgtgc ttgtgcagat
     2701 gagtcccttt gtgaggctcg accaccacaa caaagacttc cgagcaccgt gattctgaac
     2761 ggtctatcac gatatcattg atgtgagtgg gaaacctata gcgcttgaga ttctcggttt
     2821 tatagacgta ttgcatgaga ttggcgcact cctttcagag agactgtgct ggaatccttg
     2881 aattgtgtca tttcggatga agttccggct ttgcagaatg aaaaaagaaa tccctcaagg
     2941 ttccatagac ccttgtatct caagagagat tcttcacttc actcgcaaag ggtcgctctg
     3001 acactgctct tgccgtaaga acaggactac tggccgccgg tctttttctg gacttttgcc
     3061 caggaatccc gaaggctaac cgtccgattg aaaaccaaga gct
//
LOCUS       DAOQUC010000325         3088 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_36741_length_3088_cov_3.063963, whole genome shotgun sequence.
ACCESSION   DAOQUC010000325 DAOQUC010000000
VERSION     DAOQUC010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3088
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36741_length_3088_cov_3.063963"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="PKH07_06790"
     CDS             complement(<1..258)
                     /locus_tag="PKH07_06790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34685.1"
                     /translation="MMRFLATGLMVLMFLSLSIRVSALEVISSYSRPDRNSGADRQVW
                     EGFFWAEGWNADEQFYPMHFKPAGSLHVFLRNNTEKTQTLSL"
     gene            complement(511..1662)
                     /locus_tag="PKH07_06795"
     CDS             complement(511..1662)
                     /locus_tag="PKH07_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013084313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNT34686.1"
                     /translation="MANIKTAVIGTGFIGPAHIEGIRRLGYVDVVALAEENDQVARQK
                     ADALCIPQAFGNYRDLLADKSIQAVHICTPNFLHYEMVKAALEAGKHVVSEKPLAMNS
                     KESGELVAMAREKGLQNAVNFNYRFYPLVQEARQKIAAGEPGKVRLVHGSYLQDWLLF
                     DTDWNWRLEPGVGGDSRAVADIGSHWCDLLTYITGQKIVEVYAKLDTIVPSRKKPKGR
                     VETFTGSGTGDFEQVAIKTEDTASVMFKMFNGAVGNFMVSQVSPGRKNRLYFEIDCER
                     MSFVWDQENPDSMWIGYRDKANQVLMRDGSLLSPQAAAFAHYPGGHPEGYPDLLKNFF
                     DRFYAPIAKGTPIKTGELPPYADFAAGDYEVRVVEAILTSNKKQAWVSL"
     gene            complement(1706..2239)
                     /locus_tag="PKH07_06800"
     CDS             complement(1706..2239)
                     /locus_tag="PKH07_06800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34687.1"
                     /translation="MNETPLKKLYGKLKNLSSKELSELALAIQSEAQRKQNPLRRERD
                     RVLARIQKLESQVEQEKLRLEKIQSELGEIKSATSPRIQKASKRRKGVRCSGALAGAR
                     EGSLRWHMAESAKASGFPKLFTTHELFDVIKEKVKAKSEKSLWAQFVATLSRCPEFRN
                     VTRGRGAKRGKWRIVSE"
     gene            2399..>3088
                     /locus_tag="PKH07_06805"
     CDS             2399..>3088
                     /locus_tag="PKH07_06805"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HNT34688.1"
                     /translation="MTQVALGIDLGGTDIKAGTVDRSGRVLNRWKKSTEARGGAQVIL
                     QRMIELAEEIIQQSKSEGMEIVGIGIGSPGVIDYKNGIVVKMIDNLPGWSGMRLAERF
                     QERLGYPTRIDNDANVCALAECLFGAGQGKSDIVCYTLGTGVGGGIVIDRKVHHGAHG
                     YAAELGHDTVELDGLPCSCGNIGCVEMYASARAIALYAQERLRAGDQSLMSQTAESIE
                     KVTSKTVYQAAV"
BASE COUNT          743 a          828 c          791 g          726 t
ORIGIN      
        1 caacgagagt gtctgcgtct tctcggtgtt gttgcgcaga aagacatgca gactccctgc
       61 cggtttgaag tgcatcgggt agaactgttc gtctgcattc catccttctg cccagaagaa
      121 tccttcccaa acctgcctgt cagcacccga gttgcgatcg ggtcggctgt aggacgaaat
      181 gacctcaaga gccgataccc tgatggagag agatagaaac atcaacacca tcaaaccagt
      241 tgccagaaat ctcatcatac ttactccttt cgctcccaag aatgtgactc cacaggagac
      301 ggcttatttg acgagcgagc acacgaaata aataggcact gccatcacac cttttggatg
      361 cagaacttcc tggaaaggca ggcttgaaaa gcaccatcat gacactctgc gggaaccaga
      421 aaatgaggcg aaacgcctcg tctgcaaaaa agatcgtagt catgaagtct gcaccgcaaa
      481 agaagagccc tgcctgccag acaagacgtg tcagagagaa acccaggcct gcttcttgtt
      541 gcttgtcagg atcgcttcga cgactctgac ctcgtagtcg cctgctgcaa aatcagcata
      601 gggaggcagt tctccggtct taatgggagt tcccttggcg attggagcat agaagcggtc
      661 aaagaagttc ttcagcaaat cggggtatcc ttccggatgg ccgcctggat agtgtgcaaa
      721 cgctgcggct tgcggactga gcagtgagcc atcgcgcatc agaacctgat ttgccttgtc
      781 gcgatatccg atccacatgg aatcgggatt ctcctgatcc cagacaaaag acatgcgctc
      841 gcagtcgatc tcaaagtaca gccgattctt tctgccaggg ctgacctgtg agaccataaa
      901 attgccgact gcaccattga acattttgaa catcaccgaa gcggtgtcct cggtcttgat
      961 cgccacctgc tcgaagtcac ctgtacccga accggtgaaa gtctccacac gacccttcgg
     1021 cttcttgcga ctcggaacaa tggtgtccag tttggcgtac acttcgacaa ttttctgacc
     1081 cgtgatgtag gtgagaaggt cacaccaatg tgagccgata tcggccacag cacgggaatc
     1141 accaccaaca cccggctcaa gtctccagtt ccaatcggta tcaaaaagca accagtcctg
     1201 cagataggaa ccatgaacca aacggacttt gccaggctca cccgcagcga tcttctgtct
     1261 ggcttcctgt accagcggat agaagcggta gttgaagttc actgcatttt gaagcccctt
     1321 ctcacgcgcc atagcaacca actcacccga ctccttcgag ttcatcgcaa gtggcttctc
     1381 cgaaactacg tgtttgccag cttccaaggc tgccttgacc atctcatagt gaaggaagtt
     1441 cggagtgcag atgtgaaccg cctgaatgga tttgtcagcc aagagatccc gatagttccc
     1501 gaacgcttga ggaatgcaaa gtgcatccgc tttctgtcgg gcgacttggt cgttttcctc
     1561 tgcaagcgcc acaacgtcca cgtagcccaa acgcctgatt ccctcgatgt gcgctggtcc
     1621 gataaaacct gttccgatga ctgctgtctt aatgtttgcc atatccctgc tcctcctgtg
     1681 gaagtagact acagatgaga gtgtttcact cagaaacgat ccgccacttg cctctcttgg
     1741 caccacgacc gcgagtgaca ttcctgaact ccggacagcg agaaagtgtt gcgacaaact
     1801 gtgcccaaag actcttctca gacttcgctt ttaccttctc cttgatcaca tcaaacagct
     1861 catgagtggt gaaaagtttc gggaatccag aggcctttgc ggattcagcc atgtgccacc
     1921 tcaggcttcc ctcacgagct ccagccaatg cgccagagca cctgactccc tttctgcgtt
     1981 tcgatgcctt ctggattcgg ggagatgtag cagatttgat ctcacccagc tcactctgaa
     2041 tcttctccag cctgagcttc tcctgctcga cttgcgattc gagtttctgg attcttgcca
     2101 gaacgcgatc cctttcccga cgaagaggat tctgcttgcg ctgcgcttcc gattggatcg
     2161 ccaatgcaag ttcgctgagt tcttttgagg aaaggttctt cagctttcca tacaacttct
     2221 tcagtggcgt ctcattcacg atagtgtcct cctgcggttg gcttgtgtcc gctgtagcta
     2281 aaggtaacag aactctttgc cctgtcaagc attgtgtgat atctaataca ggctgacaat
     2341 agatgctgct atcagcatca aacatttata caagccctat ggtgggagga tgtcattgat
     2401 gacacaggtg gcacttggca tagaccttgg cggtacggat atcaaggcgg gaacagtcga
     2461 tcgaagcgga cgggttctga atcgctggaa gaagtcaaca gaggctcgtg gcggagctca
     2521 ggtgatcctt cagagaatga tcgaacttgc agaagagatc atccagcagt ccaagagcga
     2581 aggcatggag attgttggca tcggaatcgg aagtccgggt gtcattgact acaagaacgg
     2641 cattgttgtg aagatgattg acaaccttcc tggatggagt ggcatgcgcc tcgctgaacg
     2701 gttccaggaa cgcctgggct atccgacccg aattgacaat gatgccaacg tgtgcgcttt
     2761 ggccgaatgc ctgtttggag cgggtcaggg aaagtcggac atcgtctgtt acaccttggg
     2821 aacgggtgtt ggcggtggga tagtcatcga tcgaaaagtt catcacggag cacatggata
     2881 tgcggcggag ttgggacacg acaccgttga actggacggg ttgccttgct cctgcgggaa
     2941 cattggttgc gtcgagatgt atgcttccgc tcgggcaatc gcgctgtacg cgcaggagcg
     3001 acttcgtgca ggagaccaga gcctcatgtc tcagacagct gagagcattg agaaagtgac
     3061 ttccaaaacc gtctaccaag cagcggtc
//
LOCUS       DAOQUC010000326         3086 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_36784_length_3086_cov_2.830749, whole genome shotgun sequence.
ACCESSION   DAOQUC010000326 DAOQUC010000000
VERSION     DAOQUC010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3086
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36784_length_3086_cov_2.830749"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3086)
                     /locus_tag="PKH07_06810"
     CDS             complement(<1..>3086)
                     /locus_tag="PKH07_06810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34689.1"
                     /translation="SFVDENTTVFDWAMPNRNDDWDFWVDLNADSVFTPGVDPVVIDV
                     NANGIADVNDHVMFEGNVFGSTLQLVGKNDDEDAWFGDYPRVPESSRVFWPRVDEVIK
                     EDEGEGNIWANTKGYYDGTSLDETQIGDEVLFFGAQANLDAYGNEVSDAAEDDRTTPT
                     TEWSSQWVNADFWTATYNRTQPDSAFAATRAFFDHVENGYYLTMTDPVVVIRDSGSVK
                     GVIDTGDRLISYPGSSSNPLPLNNVNNLANRFLLMKGINVRGLNDDYLVNHPRTDGGI
                     ADLFAGNPALLTQAETLLANPIAQAALGISDPTEFFRIPIGDAYLERGTNAPGFDAGV
                     DEIWIDINLDGRLTIADVNGCERFPGLRPPSSGTFTQVADTTNLALSQLRDVISSNSN
                     LFLEESEERYNGIDDDGDGYVDEDCSDFRLFLLIDEDDYDFYVDTDGVSPFDPNTDPY
                     YVDLNADGVYQEGEWVNADDLGISDPLSFDVEYLLQAGIVRLDNDSDGRENEDPKRRV
                     GNDPNVTLKPIHAELIDEDGLDLFDNDGDGLIDEDCWFVPLFPLVDEERLDYFVDQEG
                     GVPGVFNNGIDEYYVDLNGNGLYDPIVAYFKGGYYRQEKECVYPGRDGTPDIPAGFAF
                     NVLMGVDNDRDGLVNEDVRDPNMQIYDFDSNYSDDRQDGFQLWADTDGNGRFDRTVDT
                     IISNGYSWQRILPSGVPTNTADFSNGINEDYLVRVSVDTNRTACIVPITDDGNFDFFM
                     VIRTDSGDSGGAPSGADKEGDTKELKLADLSIFGVDWGDDFSLQAVTDGVRVILNNNS
                     ARGGMSIAQEQTKRQPCLFYAADVLGFYGTTPNNVFDDDDDGVVSEEYLNGLDDDGDG
                     EDDDGNPLTPARADGLDNNNNGFIDEGIDKDYRHLFANNLDDDGDGEPGSPVDFFTGK
                     MMADGIDNNGDGRIDEGIDEEIPNGYDDDGDGEDDDGNPFTRARADGIDNDGDGLIDE
                     GIDEDCGDYGVGHASTHGSVQGYWGGWQPEAVHQFGGIPYVDATSAPIPLLGLNVT"
BASE COUNT          737 a          900 c          706 g          743 t
ORIGIN      
        1 cgtcacattc agcccgagaa gcggaatggg tgcactggtt gcatccacgt aaggaattcc
       61 gccgaactga tggacggcct caggctgcca tcctccccag taaccctgaa cggaaccgtg
      121 tgtgctggcg tgtccgacac cgtaatcacc gcagtcttcg tctatccctt catcaatgag
      181 accatcccca tcgttgtcaa taccgtcagc ccgcgctcga gtgaacgggt tgccgtcatc
      241 gtcttcccca tcgccgtcat cgtcgtagcc gttggggatt tcttcatcta tgccttcatc
      301 aattctacca tcaccgttgt tgtctatacc gtcagccatc atcttgccgg tgaaaaagtc
      361 aactgggctt cccggctctc cgtcgccatc atcatccagg ttattcgcaa agagatgccg
      421 atagtccttg tcaatccctt catcaatgaa gccgttgtta ttgttgtcca gaccatccgc
      481 gcgtgctgga gtgagaggat tgccatcatc atcctcacca tctccgtcat cgtccaagcc
      541 gttgaggtac tcctcagaga caacaccatc gtcgtcgtcg tcaaagacgt tgtttggagt
      601 cgtgccgtag aagcccagga catccgccgc atagaacaga catggttgcc tcttggtctg
      661 ctcctgcgca atgctcattc caccccgagc gctgttgtta ttcaggatga cgcgcactcc
      721 atcggtaacg gcctgcaaac tgaaatcgtc gccccagtcc actccaaaaa tggatagatc
      781 cgccagtttg agttccttgg tgtcaccctc tttgtctgcc ccggatggcg caccacccga
      841 atcaccggaa tctgtcctga tgaccataaa aaagtcaaaa ttcccatcat cagtgatcgg
      901 aacaatgcac gccgtacggt tggtgtccac cgaaacgcgt accagatagt cttcattgat
      961 tccattgctg aaatcggctg tgttcgtcgg aacacctgag ggaagaatcc gttgccacga
     1021 atatccattc gagataatcg tgtccacagt ccggtcgaat cggccattgc cgtcggtatc
     1081 ggcccacagc tgaaagccgt cctgtctgtc atcactgtag ttgctgtcga aatcgtaaat
     1141 ctgcatgttg ggatcgcgaa catcttcgtt gaccaagcca tcgcgatcat tgtcaacacc
     1201 catcagtaca ttgaatgcaa accccgcagg aatatccgga gtgccatcgc gtcccggata
     1261 cacacattcc ttttcctgac gatagtatcc acctttgaaa taggcaacaa tggggtcata
     1321 gagtccattg ccgttcagat caacgtagta ttcatcaatg ccgttgttga agactcccgg
     1381 cactccccct tcctgatcca caaagtagtc cagacgctcc tcatccacca ggggaaacag
     1441 aggaacgaac cagcagtctt catcgatcaa cccgtctcca tcgttgtcaa ataggtcgag
     1501 tccatcttca tcaataagct cagcatgaat cggtttcagt gtcacgtttg gatcattgcc
     1561 gacgcgacgt ttgggatcct cattctcccg accatcagaa tcgttgtcga ggcgaacaat
     1621 ccctgcctgc aaaaggtatt caacgtcaaa actgagagga tcggaaatgc ccagatcgtc
     1681 tgcattgacc cattcaccct cttgatagac tccatcggca ttcaagtcca catagtaagg
     1741 atcagtattg gggtcaaagg gactcacacc atccgtatcc acgtagaaat cgtagtcatc
     1801 ttcgtcaatc aacaagaaca gccggaaatc ggaacagtcc tcatctacat agccatcccc
     1861 gtcgtcatca attccgttgt accgttcctc gctctcctca agaaagagat tcgaattcga
     1921 ggagatgaca tctcgaagtt ggctcagtgc aaggttggtc gtgtctgcta cttgagtgaa
     1981 cgtgccactg gaagggggtc tcaagccagg aaaccgctcg catccgttga catcggcaat
     2041 agtcaacctg ccgtccaagt taatgtcaat ccagatttcg tcaacaccag catcaaagcc
     2101 aggggcattg gttcctcttt ccaggtaagc atctccaatc gggatccgga agaactcagt
     2161 cggatcgctg atacccaaag ccgcctgtgc aattggattt gccagaagag tttcagcttg
     2221 ggttaaaaga gccggattcc ccgcgaagag gtcggcaatt cccccgtccg ttcgggggtg
     2281 attcaccaga tagtcgtcat tcagaccacg gacgttgatg cccttcatga gcaggaatcg
     2341 gttcgccagg ttgttgacgt tgttcaaggg gagcgggttg ctggaagaac caggatagga
     2401 aatcaggcga tcccctgtgt caataacccc tttcaccgac ccgctatcac gaatgaccac
     2461 aaccggatcg gtcatagtca aatagtatcc attctcgaca tgatcaaaaa acgcccttgt
     2521 cgctgcaaag gcggagtccg gttgagtgcg attgtacgtg gccgtccaga aatcggcgtt
     2581 gacccactga ctgctccact ctgtggtcgg cgtggtgcgg tcgtcctcag ctgcgtcgga
     2641 aacctcgttt ccataggcgt cgagattcgc ttgcgcccca aagaacagga cttcgtcacc
     2701 gatctgagtc tcgtcgagtg aggtaccatc atagtagccc ttggtgttcg cccagatgtt
     2761 cccctctccc tcatcttcct tgataacctc atccactctt ggccagaaga ctctggaact
     2821 ctcgggcacg cgaggatagt caccaaacca ggcatcctca tcatcattct tgccaaccaa
     2881 ctgcaatgtg ctgccgaaga cattgccttc aaacatcaca tggtcattga catcggcaat
     2941 accgtttgcg ttgacatcaa tgactaccgg atccactcca ggagtgaaaa cgctgtctgc
     3001 gttcagatct acccagaaat cccagtcatc gtttctgttc ggcatcgccc agtcaaagac
     3061 ggtcgtattc tcatccacaa aactat
//
LOCUS       DAOQUC010000327         3081 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_36879_length_3081_cov_2.828486, whole genome shotgun sequence.
ACCESSION   DAOQUC010000327 DAOQUC010000000
VERSION     DAOQUC010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3081
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36879_length_3081_cov_2.828486"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1816
                     /locus_tag="PKH07_06815"
     CDS             <1..1816
                     /locus_tag="PKH07_06815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34690.1"
                     /translation="WSGNAILQQGTLQGGVLIDALSDVTAEISLQSNQIQEFLLRWKR
                     ANVPGRVSYSQKQGRHSLIFRSDSQLEELRRFGQKYLGWDARDFQLAGSVFSDVETSW
                     LTGASDQASVKGRIDFEGCQVGHKNWPLPVESLEGLVKIDDSSFVSDRLSFTSGKASV
                     AIKATYKKGSFSMESLESVQLADVMGKLQQLYPTRYTGWSAGGEIGFTLQSEPPRVED
                     GTAALRKYEGDLSFYEAFVKTDRMDQPVEKIRGMVHFTRNSAQAEDLVFAFAGTEGTC
                     SLTVDDFDQPVVVGEVLAANLDISRLLEMLKFRSMATPQSKPPGLHLQRVSGLVKADK
                     FKFSSLSGTALSTRFEWTDQDEISSLNLPEIAFSAWAGNATGAFEAGMSESNLIYSGT
                     LEANAISLRDMLVAMGTSSPGQVDGEFDLVIDYTGTGLTPDGFEARGEFEARNVTSLR
                     HDFFSRLSEAVKLDLFREITFDRVYAPFLITNKRIFTDDLRWIGRFSEMQWKGSIGMD
                     QTLNYDIKAAISASGASGTSPIEGTPAGPLFRFLTAKSIDLSLTGTLKEPKFAVRPLK
                     TLIQPAKDVKDIVIVPVGKVINTLRSLPPSTNRKSAPR"
     gene            2084..>3081
                     /locus_tag="PKH07_06820"
     CDS             2084..>3081
                     /locus_tag="PKH07_06820"
                     /inference="COORDINATES: protein motif:HMM:NF012340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HNT34691.1"
                     /translation="MRNTRPTRMRSWLFVAVLTVLIPSASAQTARIAFEKNDTLEEIR
                     QKIEANGYAFQVEPNWVYKLPESDRQRLLSRRASPKPLKPLDEPDYGPLMQVLSRQNG
                     LPSSFDWRSQGGHTYIGAVRNQGDCGSCYSFGATAALESVYNINNGLYDGNCVDLSEA
                     FLAFCLGELPAYYDHFYGCDGADYDYLELQALAEYGLPLESAFPYSDSLTACQQDSWN
                     ASRYSVQSWHRVPCADVNAIKTAIYNYGVVDAAVLAGDAFMAYSTGVYDDTNASCDTT
                     PCYYAETNHAIALVGWNDDPVDGGYWILRNSWGTSWGESGYMRIKYQAAHVSCAVC"
BASE COUNT          698 a          762 c          873 g          748 t
ORIGIN      
        1 ctggtcaggg aacgcaatct tgcagcaggg aactcttcag ggtggagtgc tcatagatgc
       61 actcagcgat gtgaccgccg aaatctccct gcagtccaat cagattcagg agtttcttct
      121 ccgctggaag cgggcaaatg ttccaggaag ggtgtcctat tcacagaaac agggacgaca
      181 cagtctcatc tttcgatcgg attctcagtt ggaggagcta aggcgcttcg ggcagaagta
      241 cctgggttgg gatgctagag attttcagct tgcagggagt gtcttttcgg acgtcgagac
      301 gagttggttg actggcgcat cggatcaggc ttctgtgaaa ggaaggatag atttcgaggg
      361 atgtcaggtt ggtcacaaaa attggcctct gcctgtggag agtctggagg ggttggtcaa
      421 gatagacgat tcttccttcg tctcagaccg tttgagtttc accagcggga aagcctctgt
      481 ggcaatcaag gcaacgtaca agaaaggctc tttcagcatg gagtcgcttg agagcgtgca
      541 gttggccgat gtgatgggca agctgcaaca actctatccg acgcgctaca caggatggtc
      601 ggctggtgga gagatcggat tcactctgca gtccgaacca ccccgagtcg aagatggaac
      661 tgccgccctg agaaagtacg aaggggatct gtccttctat gaagcttttg tcaagactga
      721 tcgaatggat caacccgtgg agaagattcg ggggatggtt catttcacgc gcaattcagc
      781 acaagcggaa gatctggttt tcgcctttgc tggaacggag ggaacctgtt ctctgaccgt
      841 tgatgatttt gatcagcccg ttgtggtggg agaagtgctg gcggcaaatc tggatatctc
      901 ccgactgctt gagatgctga agtttcgctc catggcgaca ccgcagagca agcctcctgg
      961 gctccacttg cagcgtgtgt ccggcctcgt gaaggcagac aaattcaagt tttccagtct
     1021 ctctggcaca gcgttgagca ctcgatttga gtggacagat caggatgaga tttcttctct
     1081 gaatctccct gagattgcat tttcagcttg ggcgggaaat gcaacgggcg cgttcgaagc
     1141 agggatgagc gaaagcaatc ttatctactc tggaacgctg gaagcgaatg ccatttccct
     1201 cagggacatg cttgtggcca tgggaaccag cagcccgggt caggtggatg gcgagtttga
     1261 cctggtgatc gactacactg ggacgggatt gactccggac ggcttcgaag ctcggggaga
     1321 gtttgaggca cggaatgtga cttcgcttcg acacgatttc ttttcgcgtt tgtcagaagc
     1381 cgtgaaactg gatctctttc gggaaatcac ttttgacaga gtttacgccc cgttcctgat
     1441 caccaacaag cgtatcttta ccgatgacct tcgttggatc ggacgattca gcgagatgca
     1501 gtggaaaggc tccatcggga tggatcagac tctcaactat gacatcaagg cggccatttc
     1561 agccagtggc gcgtcaggga caagcccaat tgagggaact cccgccggtc cgttgttccg
     1621 gtttctcacg gccaagtcca ttgatctgag cctgacaggg acactaaaag agccaaagtt
     1681 tgcggtgcgc ccgctgaaaa cgctgatcca gcccgccaaa gatgtcaaag acatcgttat
     1741 agtgcctgtt ggcaaagtga taaacaccct gagaagtctt cctccgagca caaatcgcaa
     1801 gagcgctcca cgttgaacag accttacctt tcgttctgcg ctcccttgtt cgcatggtgc
     1861 cgaaccccgc ttgttctcaa ggtttgatat gactgcccgc atctacaccc cgtgtctttt
     1921 gtcgctcccg ataggactca gtccagaaga agcgtgtcat ttgccaagca agcgtccctg
     1981 agagtgtgca gagtccacgc gcccgaaggt gctaacccgc tcggtatttg acaaagattc
     2041 ggtcagtcct tcatagtatc cgaaaacaga gagggaacga acaatgagaa acaccagacc
     2101 gacaaggatg agatcttggc tttttgtggc tgtgcttact gtgctgatcc cgtctgcctc
     2161 agcacagacg gcccgaattg cgttcgaaaa gaacgacaca ctggaggaga ttcggcagaa
     2221 gatcgaagct aacggctatg cctttcaggt tgagcccaac tgggtgtata aactgcctga
     2281 atctgatcgg cagagacttc tcagcaggcg tgcgtcgcca aaaccgttga aacctttgga
     2341 tgaaccagac tacgggcctt tgatgcaggt gctctcgcgt cagaacggtt tgccttcttc
     2401 ttttgactgg cgcagtcagg gtggtcacac atacatcggg gcggttcgaa accagggtga
     2461 ttgcggttcc tgctacagtt tcggcgccac cgcagcgctt gagtctgtct acaacatcaa
     2521 caatggtctg tatgatggca actgcgttga tctgtctgag gctttcctgg cattctgtct
     2581 tggggagctg cccgcctact atgaccactt ctacggatgt gacggagcag actacgacta
     2641 tttggaactg caggcgttgg cggagtatgg tcttcctctt gagtccgcgt ttccgtactc
     2701 tgattccctg accgcatgtc agcaggattc ctggaatgca tcaaggtatt ctgtgcagag
     2761 ttggcatcga gttccgtgtg ccgacgtgaa cgccatcaag actgcgatct acaactacgg
     2821 agtcgttgat gcggcagtct tggcaggtga tgccttcatg gcttactcaa caggtgtcta
     2881 cgatgacacg aatgcgtcct gcgacacgac gccttgttac tatgccgaaa ccaatcatgc
     2941 cattgcgctt gttggatgga atgatgatcc cgtagatggc ggatattgga ttcttcgcaa
     3001 cagctgggga acgtcatggg gagagagtgg ctacatgcgc atcaagtatc aggctgctca
     3061 cgtttcctgt gcggtctgct a
//
LOCUS       DAOQUC010000328         3079 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_36909_length_3079_cov_3.478505, whole genome shotgun sequence.
ACCESSION   DAOQUC010000328 DAOQUC010000000
VERSION     DAOQUC010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3079
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36909_length_3079_cov_3.478505"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1501
                     /locus_tag="PKH07_06825"
     CDS             <1..1501
                     /locus_tag="PKH07_06825"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNT34692.1"
                     /translation="LGFVCKDLSDYGVTWDEAKPNFEAALRQADWFSRLGELERPFSR
                     ETIDKYWKTTSDHPSLPRTLAALSYLGLNRILGTIPALRFPNAVWFALMAAVVFHWTA
                     SKWTIQAGFAALAALLLMPRLFSEAHILSLDLPITALWVLTAWAFVKGLESWGWGVAT
                     GILYAIAVTTKLHAFFLPFPLVAWTLWRAHKHWYRPVLPMAVLAPVFYVGLQPWLWFD
                     TGTRLAERFFHYADKSVGNPIKLFYFGQLYSNNTPWHYSIVLILVTVPILTLVLSLIG
                     ISRVWVHRHAEPDGILLLLLFLVPPCLLFLPRAQGYDGTRLFMECFPFLACLSGIGWE
                     QLRTRMLHKVGLDRMKARRILIVLTFVWLIPICVQMVLLHPCQLGYYNELIGSLPGAR
                     LVGMESTYWCDAITPDLIEEMNTALPENSHVVTMSMSVAVLPFYQQVGLLRSDLRLDV
                     PDTPADFHLLQCRQGMFGQIEWYYYKTRTPIYRVERFGVPLMLLFSEEQ"
     gene            1498..2709
                     /locus_tag="PKH07_06830"
     CDS             1498..2709
                     /locus_tag="PKH07_06830"
                     /inference="COORDINATES: protein motif:HMM:NF014177.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0032775 - DNA methylation on adenine
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HNT34693.1"
                     /translation="MKHSIPTLHVMRYMGNKQKLLPQIISAVEKHAPPGSIIVDLMAG
                     THAVGYSLKSQYRIYANDVQKYTLPIGKALLCSDGISVSSEKAKCDLEAFYEENQEKR
                     IANFFQQHYSDTYLSARQCAELDSIRYAIERYREDLPNPDRSDLYLTALISTVCNAQS
                     TPGHFAQFLPPNHQRTQPLRQIRLWSQFLRYADKLGIHQTPSLHPLLLPIEKGSDFRD
                     MLTDQEWSGNANRVFAHSWREFFERKDILEEASEFGLIYVDPPYSQEQYSRFYHFLET
                     LVLLDQPQTRFKARYREGRFMSDFCYRGRVASEFDALLRTTSTVCCCPVLISYGSKGL
                     LPKDRLLEICQRHYEIVQLEEIPYAHSTLGKGNVPDVLELLLSCSRPKNVRASSRHTE
                     LQLSRDDQRTA"
     gene            complement(2816..>3079)
                     /locus_tag="PKH07_06835"
     CDS             complement(2816..>3079)
                     /locus_tag="PKH07_06835"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNT34694.1"
                     /translation="QEALREFCSHPNRFDVIVTDLTMPTMTGLELADRIRRVRPDVPI
                     ILCSGYAEAVPKEVSQESDITLCLRKPMTSLNLGRAIQRLVRR"
BASE COUNT          664 a          877 c          764 g          774 t
ORIGIN      
        1 gctcggtttc gtatgcaagg atctctcgga ttacggagtg acttgggatg aggcaaaacc
       61 gaactttgaa gcagcactca gacaagccga ttggttcagc cgcctgggag aactggaacg
      121 tcccttctcc cgcgagacca tagacaaata ctggaaaaca acgtccgatc acccgtcact
      181 gcctcgaaca ctggcggcat tgtcgtatct cggactgaac cgcattctgg gaaccattcc
      241 cgcactgcgt ttcccgaatg ccgtttggtt tgccttgatg gcggctgttg tctttcattg
      301 gacggcaagc aagtggacga ttcaggcggg atttgcggcc ttggctgctc tgttgttgat
      361 gcctcgcctt ttttccgagg cacacattct gagtctggat cttcccatca cagcgctttg
      421 ggtcttgact gcctgggcgt tcgtcaaggg tctcgaatca tggggttggg gtgtggcgac
      481 gggcattctc tacgcaatcg ctgtaacgac gaaactccat gcgttcttcc ttcccttccc
      541 gctggtggct tggactcttt ggagagcaca caaacactgg taccgtccgg tgcttcccat
      601 ggcggtgctt gcaccggttt tctacgtcgg tctgcaaccg tggctctggt ttgatacagg
      661 aacgcgactg gcagagcgtt tctttcacta cgccgacaaa tcagtgggca atcccatcaa
      721 actcttctac tttggacagc tgtactcgaa caacacccct tggcattact caattgtcct
      781 gattctggtg acggtgccga ttctcacgct ggtgctttct ttgattggta tctcccgtgt
      841 ttgggttcac cgtcacgctg aaccagacgg catcctgctt ctgttgcttt tccttgtgcc
      901 tccctgtctt ctctttctcc cacgggcaca gggttacgat ggcacgcgac tcttcatgga
      961 gtgctttccg tttctggcct gtctctcggg catcggatgg gaacagttgc gaactcggat
     1021 gcttcacaag gttggtcttg accggatgaa agcgcgaagg atactcatag tcctgacgtt
     1081 cgtctggctg attccgatct gcgtccaaat ggtgctgctg cacccctgtc aactggggta
     1141 ttacaacgaa cttatcggca gccttccggg cgctcggctc gttggcatgg aaagcactta
     1201 ctggtgtgat gcgatcaccc ctgatctcat tgaagaaatg aacacagcac tgcccgaaaa
     1261 ctcgcatgtc gtcaccatgt ccatgagcgt tgcggttctg cctttctatc aacaggtcgg
     1321 tctgctgcga tccgatctgc gcctggatgt tccagacacg ccagccgatt tccacctgct
     1381 ccagtgccgc cagggaatgt tcgggcagat cgaatggtat tactacaaga cacgaacccc
     1441 gatctacaga gtcgaacgat tcggagttcc actgatgctt ctgttcagcg aagagcaatg
     1501 aaacactcga ttcccacact tcacgtcatg cgctacatgg gcaacaagca gaagctgctg
     1561 ccgcaaatca tcagcgccgt ggaaaaacat gcaccaccgg gaagcatcat cgtggacttg
     1621 atggcaggaa ctcatgccgt tggctattcc ctgaagagcc aataccgcat ctacgccaat
     1681 gatgtgcaga aatacaccct tcctatcgga aaggcactcc tgtgctctga cggtatctcg
     1741 gtttcctcag aaaaggccaa gtgcgatctc gaagccttct atgaggagaa tcaggagaag
     1801 cgcatcgcca acttctttca gcaacactac agcgacactt atctcagtgc tcgacaatgc
     1861 gcggagttgg actccattcg ttatgccatc gaaagatatc gcgaagattt gcccaatcca
     1921 gaccgttcgg atctctatct gaccgcactg atttcaaccg tctgcaacgc acagagcaca
     1981 cccgggcatt tcgcccagtt tcttccgccg aatcaccaac gaacccagcc tcttcgtcaa
     2041 atacgcctct ggagtcagtt cctcagatat gccgacaagt tgggtattca ccaaacccct
     2101 tcgctgcacc ccctcttgct ccccatcgaa aaagggtcgg atttccgaga catgctcact
     2161 gaccaggaat ggtctgggaa tgccaatcgg gtctttgcac acagctggcg tgaattcttc
     2221 gaacgcaagg atattctcga agaggcatcg gaattcggtt tgatctatgt ggatccccca
     2281 tactctcagg agcagtattc ccgtttctac cactttctgg aaacgctggt tctcctggat
     2341 caacctcaga cccgattcaa agcgcggtac cgcgaaggcc ggttcatgtc cgacttctgc
     2401 tatcgtggga gagtcgcttc ggaattcgat gcacttctgc ggacgacttc aaccgtgtgc
     2461 tgctgtcccg ttctcatcag ctatgggagc aagggtcttc tacccaaaga caggctcctg
     2521 gagatttgcc agagacacta cgagatcgtt cagcttgaag aaatccccta cgcccattca
     2581 accttgggca agggaaatgt tccagatgtc ctcgaacttc tgttgtcctg ttccagaccc
     2641 aaaaacgtgc gggcatcttc ccgacacacc gaacttcagc tcagtcggga tgaccagcgc
     2701 accgcatagc tttgcctcta tctttcgcaa actctctgtt tcagcatgtg gaaatgcagt
     2761 ggatgttctg ccccctgttg cgtgcagaat gaacgtcttg tctttgtcga gacaatcatc
     2821 gccgcacaag tctctgaatt gcccgtccga gattcagact tgtcatgggt tttctcaggc
     2881 agagtgttat gtcagactct tgactcactt ctttgggaac agcttccgca tatcccgaac
     2941 agaggatgat cggaacatcc ggccgaacgc gccgaatcct gtctgccagt tcaaggccgg
     3001 tcatggtggg catggtgaga tcggtgacaa tgacatcgaa ccgatttgga tggctgcaaa
     3061 actcccgcaa ggcttcctg
//
LOCUS       DAOQUC010000329         3078 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_36930_length_3078_cov_3.230566, whole genome shotgun sequence.
ACCESSION   DAOQUC010000329 DAOQUC010000000
VERSION     DAOQUC010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3078
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36930_length_3078_cov_3.230566"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="PKH07_06840"
     CDS             complement(<1..228)
                     /locus_tag="PKH07_06840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34695.1"
                     /translation="MICTQMTESQRIIIQFSTRVVSVTTGGGVGMAVFVGVQEGVWDG
                     VIVGVLVGVIVAVGVRVGVSVGVCVGVLVEVG"
     gene            552..1637
                     /locus_tag="PKH07_06845"
     CDS             552..1637
                     /locus_tag="PKH07_06845"
                     /inference="COORDINATES: protein motif:HMM:NF017847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HNT34696.1"
                     /translation="MDIGIYGLSSSGKSVLFDLLTGLPESGDRGKTRIGSVTVPDPRL
                     DQLVNWYHSRKVTPIHLELHDPGLGHSEKSEDSLSDARLNLIRSFDIIALVVRCYESE
                     SVVHPEGTVNPSRDLDLLLTQLILADLEMIERRLVRIEESFRKLPKDDREAYEHEKQA
                     LQRIKQTLESESPWETTTLSDFEKKKVSHYSLFHMKRYVVVGNLGDCSASQDAAHWAA
                     LRESCQSHRIPCVGVNLKLELEMKDMDPSEAQEFRESMNLESESCRQLVKGLYDQLGM
                     TTFYTAGEKESAARCVVQGTLAPQAAGTIHSDLERGFIRAEVFSFDDLVKAEGSEPKV
                     KQLGCLRIEGKDYVVQDGDIVLIRFSA"
     gene            complement(1727..2662)
                     /locus_tag="PKH07_06850"
     CDS             complement(1727..2662)
                     /locus_tag="PKH07_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008245117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045121 - membrane raft [Evidence IEA];
                     GO_process: GO:0034982 - mitochondrial protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stomatin-like protein"
                     /protein_id="HNT34697.1"
                     /translation="MNAGTVILIVVAFFALIVLFKSIRIVPQKSAFIVQRLGKYHSTL
                     EAGFHILLPFVDALAYHHTLKEQAIDVPPQTCITKDNISVEVDGILYLQVVDPQKASY
                     GITNYLFASTQLAQTTMRSVIGKLELDRTFEERDAINSLIVDAVDKASDPWGIKVTRY
                     EIKNIVPPQSIRDAMEKQMRAEREKRAMIAESEGDKQAKINRAEGDKQEMIERSEGEK
                     QKRINEAEGRAAEIEKVAVATAGGIREIAAAINERGGMEAVNLRVAEQYLTGFANLAK
                     TTNTLILPANLADIAGVIKTATAAIHSVSGGGQTA"
     gene            complement(2659..>3078)
                     /locus_tag="PKH07_06855"
     CDS             complement(2659..>3078)
                     /locus_tag="PKH07_06855"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HNT34698.1"
                     /translation="MLFLELILPGLIIFFFGVGAIVVGLICLIVEPSTNVQIALFLVT
                     SILLLLALRKKITGAFVGYVTSRGNMSKVPDEFVGKRATVVREIHPNLNGKVALHGSN
                     WEAQADETIAEGEPVEIIGRENITLRVKRVVQRGEQE"
BASE COUNT          728 a          792 c          782 g          776 t
ORIGIN      
        1 gccgacttcg accaacacgc cgacacagac accgacagag acacctactc gcacaccgac
       61 agccacaatg actccaacga gaacaccaac tatgacacca tcccagactc cctcttggac
      121 tcctacgaat accgccatcc caacaccgcc ccctgtcgta accgagacta cacgcgtcga
      181 gaattggatg atgatgcgct gagactctgt catttgagtg cagatcaaag cgcactcctt
      241 gacacaagca cattatcacc aggtcgagta ttcgctgact tgcttcctga gaatgcccca
      301 tccgaatccg ccgacgacaa ggtcgaggtt ctgaactccc tgcctgccaa aagagaacat
      361 cggagcaaca cggtgcgcag acagtcgaat agatttctgc ggaaaggttg tttttctgtg
      421 ctatgttttc agatgtctga tcttggtcgt aactccagat cattgacagg gatgtcttga
      481 aagagcgtga agcggaacgt gttttgcttc ccaaggcatt catactctac ctgtaaaggg
      541 gtttggtgtt tgtggacatt ggaatttatg gtctgtcgtc aagtggaaag tctgtcctgt
      601 ttgacctttt gacgggtctt ccagaaagtg gggacagagg caagacacga attggctctg
      661 taactgttcc tgatccgaga ctcgatcagt tggtgaactg gtatcattcc aggaaggtga
      721 ctcccattca cctggagctc catgatcccg gtttgggcca ttctgaaaag agtgaggatt
      781 cgctgagtga cgcccgccta aacctgatcc ggagttttga catcatcgct ttggtcgttc
      841 ggtgctatga atcagagtcg gtagttcatc ccgaaggaac cgtgaatccg tcacgcgatc
      901 tcgatcttct tctgacacaa ctgattcttg ctgacttgga gatgattgag agacgtcttg
      961 tccgaatcga agagtccttt cggaaactcc ccaaggacga tcgagaagcc tatgagcatg
     1021 aaaagcaggc actgcagcgc ataaagcaga ctctcgaatc tgagtcgccc tgggagacga
     1081 ccacactgag tgacttcgag aagaagaagg tgagccatta ctcactcttt cacatgaaga
     1141 gatatgttgt ggtgggaaat cttggcgact gctcggcaag tcaagatgct gcccactggg
     1201 cagcgcttcg tgagtcctgt cagagtcatc gaattccttg cgtcggggta aatctcaaac
     1261 tggaattgga aatgaaggac atggatccgt cagaagccca ggaattccgc gaaagcatga
     1321 atctggaatc cgagtcgtgc agacaactgg tcaagggtct ctatgatcaa ttgggcatga
     1381 ccacgtttta caccgcaggt gagaaggagt ctgccgctcg atgtgtggtg cagggaactc
     1441 ttgcccctca agcggcaggc acgattcact cggacttgga acggggtttc attcgtgctg
     1501 aagttttctc gttcgatgat cttgtcaagg cagagggttc tgaaccgaag gtgaagcaac
     1561 tcggctgcct tcggattgag ggcaaagact atgtcgtgca ggatggcgac attgttctga
     1621 ttcgcttctc ggcgtaggat cagctgccga ctcgaatccc ttgttcaaag aaaagaccct
     1681 gggtgatggt ggtcacgcca gggtcttctt tatctatcgc ggccgatcag gccgtttgtc
     1741 cgccgcctga gactgagtgg atcgctgcgg ttgccgtttt gattactcct gcaatgtctg
     1801 caagattggc aggaagaatc agggtattgg ttgtcttggc gagattggca aaaccggtga
     1861 ggtactgctc ggcaacccgg aggttcaccg cttccatgcc acctcgctca ttgatggcgg
     1921 ctgcaatttc acggatccca ccggcggtgg caacagcaac cttttcgatc tctgctgcgc
     1981 gaccttcggc ttcgttgatc cgcttctgct tttcaccctc agagcgctcg atcatctcct
     2041 gcttgtcgcc ttcggctcga ttgatcttgg cttgtttgtc gccttcagat tcagctatca
     2101 tggcgcgttt ctctctctcg gcgcgcatct gcttttccat ggcatctcga attgactggg
     2161 gcggcacgat gttctttatc tcgtagcgag tcaccttgat accccaagga tctgacgcct
     2221 tgtcaactgc atcaacaatg agactgttga tggcatccct ctcctcaaat gtccggtcaa
     2281 gttccaactt tccgatcaca ctgcgcatgg ttgtttgagc caactgtgtt gaggcgaaca
     2341 gatagttggt gattccgtaa gacgccttct gaggatctac cacctgaagg tagaggatgc
     2401 catctacttc gaccgagatg ttgtccttgg tgatgcacgt ctggggagga acgtctatgg
     2461 cttgttcctt taacgtgtgg tggtaagcaa gtgcatccac aaaaggcaac agaatgtgga
     2521 aacctgcctc cagcgtcgaa tggtattttc ccaatctctg tacgatgaag gccgacttct
     2581 ggggcacgat gcgtatgctc ttgaacagaa cgatcagcgc gaaaaaagca acaacgatca
     2641 gaatgacagt tcctgcgttc attcttgttc tcctctctga acgactcttt tcactcttag
     2701 tgtaatgttt tctcgtccga tgatctcaac tggctctcct tcagcgatcg tttcatctgc
     2761 ctgggcttcc cagttagagc catgtagagc gactttgcca ttgaggttcg gatgaatctc
     2821 ccgtaccaca gtggccctct ttccgacaaa ttcatctggc accttggaca tgttcccgcg
     2881 agaggtgaca tatccgacaa aagcacccgt gatcttcttt cgaagtgcca aaaggagcag
     2941 gatcgaggta accaaaaaga gggctatctg aacatttgtt gatggctcaa ctatgagaca
     3001 gatcaaacct acaactatcg ccccgacacc aaagaaaaag atgatcagac ctggcagaat
     3061 caactcgaga aacaacat
//
LOCUS       DAOQUC010000330         3071 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_37054_length_3071_cov_4.096485, whole genome shotgun sequence.
ACCESSION   DAOQUC010000330 DAOQUC010000000
VERSION     DAOQUC010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3071
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37054_length_3071_cov_4.096485"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..990
                     /locus_tag="PKH07_06860"
     CDS             <1..990
                     /locus_tag="PKH07_06860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34699.1"
                     /translation="WGIGWSHQNMFYDLQRSVARKPIFNSENHLQPDGSISYVPPEHY
                     RTALWQGAIHGQGATTIWVWERTLERSHKDYAASVPFYGNVMDRPGCAQEVGTTCLDL
                     NRFAEEVTALQNVRAPVALVYSMASYARNDAYLDFLNRAYAAINFCGVKVDFISEKQF
                     AEGKGSEYRVLILPNVTNLERSTFESLLGLPETVRLVSLGEIAIRDPYGNMFSPEESN
                     RIAERTLVLPTDEISEALWHDIRAELLRASSLPNVRVVDAETGEPVWGVEWLPAKVGD
                     RTIVNIVNLLNESREIKLMLRGETVNARDLLSFGGKREVRRLEPITPVLAEIR"
     gene            1154..2185
                     /locus_tag="PKH07_06865"
     CDS             1154..2185
                     /locus_tag="PKH07_06865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNT34700.1"
                     /translation="MRTRVVFLIVMGVFLNVAFCCWAQQTSEPAGASVSAATSDPKEL
                     SRFAQEASNRRPYGGFEQALAKIDQMLLEENYRDVVELSLLMMFRYWDDRLPLIQRCG
                     RGLVGMERWKDAIESFELVARTAMSADDVKGATEFFYQDAVANLVVTQTNYWLKQDDS
                     SSRAPESIKTNCLEILNEFPDEEFILLAHYKGWRHPEAILALGAVCVGSATPNLQSRE
                     LKLLEGLVVSTDGRKRTNLRNERGVTYSFLALRQWWVLMEQMDKPFAEKAQELEKFAN
                     RSVEDYTTPLAHVLLGRLYSSTAKESTKAVQYYKQALAMKLPPDDECWKGYHEECMDA
                     LEKLGAAEQ"
     gene            2210..>3071
                     /locus_tag="PKH07_06870"
     CDS             2210..>3071
                     /locus_tag="PKH07_06870"
                     /inference="COORDINATES: protein motif:HMM:NF014851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta galactosidase jelly roll domain-containing
                     protein"
                     /protein_id="HNT34701.1"
                     /translation="MTRRDAEGLSPSGRRVKVSLNAGWLFEKQSRGIGELGSYERRRG
                     RESETERIFVGAEQLEYDDSCWERVDIPHCWNAHDVMTAQSGYWRGIGWYRKTFRLGE
                     QWSEKRVRLQFEGVNQRVTLWLNGRVLGSHKGGYTPFEFDITEHLNPLGEVNILAARV
                     DNLYDPDMPPTVKTDINFVGGIYRDVRLEGTYPIHVSSVWVQSSKLDHKKAVIVAKTV
                     VANNSSATRVVQVLHKVFDGDGNAVCSFSGEHELVANELGEFEHRLQTVSNPRLWSPE
                     SPHLYRLLTQV"
BASE COUNT          728 a          694 c          908 g          741 t
ORIGIN      
        1 tgggggatcg gatggtctca tcagaatatg ttctatgatc ttcaacggtc cgtggcacgc
       61 aagccgattt ttaactctga gaatcatctt caaccggacg gctccatcag ttatgttcca
      121 ccggaacact atcgaacagc actctggcaa ggagcgatac acggtcaggg agcaaccacg
      181 atctgggttt gggagagaac gctcgagcgg agtcacaagg attatgccgc ttcagtgccg
      241 ttctacggca atgtcatgga caggcctgga tgcgctcaag aggttgggac tacctgccta
      301 gacctcaacc gatttgccga ggaggtcacg gcgcttcaga acgttcgtgc tccagtggca
      361 ctggtatatt cgatggcctc atacgccagg aacgatgcat atctggactt tctcaacaga
      421 gcctatgcag caatcaactt ctgcggagtc aaggttgatt tcattagtga gaaacagttt
      481 gcggaaggca agggctcaga gtacagagtc ctcattctcc caaatgtgac caatctggag
      541 cggtcaacgt ttgagtcgct cctaggactc ccagagaccg tccggcttgt gagtttagga
      601 gaaattgcca tacgagatcc ctatggaaac atgttttccc cagaggagag caaccggatt
      661 gcggagcgga cactcgttct tcccaccgat gagatttccg aagcgctttg gcatgatatt
      721 cgtgctgagc ttctccgtgc gtcaagtctt cccaatgttc gggtggttga tgctgagacc
      781 ggtgaaccgg tctggggtgt cgagtggttg cccgcgaagg ttggagacag aacgatcgtc
      841 aatatcgtga atcttctgaa tgagtctcgt gagatcaaac tgatgcttcg tggagagacc
      901 gtgaacgcaa gggatctgct gagttttgga gggaagaggg aagtccgaag gcttgaaccc
      961 atcacaccag tgttggccga gatcaggtga agcgtttttg atcgttggag tggccttgcg
     1021 agcgtggacg aacaactcgt ttgacgtcac ctgcccttgc cgatagtatc cgtgtgtgag
     1081 tctatatgtc ttcagggaac gattcaggac tccgaaccga aagttcccga aatggttatc
     1141 aagaggtggc aggatgcgaa caagagtggt cttcctaatt gtgatgggtg tattcctgaa
     1201 tgtggcgttt tgttgttggg cgcagcagac atctgaacct gctggggctt ccgtttcggc
     1261 ggcaacatca gatccgaagg agctctctcg atttgcacag gaggcttcca atagaagacc
     1321 ctatggaggc ttcgagcagg ctttggctaa gattgatcag atgctcctcg aagagaacta
     1381 tcgggatgtg gttgagttga gtctgctgat gatgtttcgg tactgggatg acaggcttcc
     1441 gctcatccag cgctgtggcc gtggtttggt tggtatggag cgatggaaag atgccattga
     1501 gagtttcgaa ctcgttgcca gaacggcaat gtcggccgac gacgtcaagg gagcaacgga
     1561 gttcttctat caggatgcag ttgccaatct cgttgtgact cagaccaact actggctcaa
     1621 acaggatgac tcttcctcaa gggcgcccga gtcaatcaag acgaattgtc tcgaaatact
     1681 caatgagttt ccggacgagg agtttattct gttggcgcac tacaaagggt ggagacatcc
     1741 tgaggcgatt ctggcactgg gggctgtttg tgtcgggagc gcaacgccca atcttcaatc
     1801 gcgggaactg aaacttctgg agggtctggt cgtaagcacc gatggacgga agcgtacaaa
     1861 tctgcgcaac gaacgtggag tgacctattc gtttctggct ttgcgacagt ggtgggtgtt
     1921 gatggaacag atggacaagc cgttcgcaga aaaggcgcaa gaactggaga agttcgccaa
     1981 tcgttctgtt gaggactaca cgacaccttt ggctcacgtc ttgcttggca ggctctattc
     2041 cagcacagca aaggagtcca caaaggcagt gcagtactac aagcaggcac ttgcgatgaa
     2101 acttccgcct gatgatgagt gttggaaggg ataccacgaa gagtgcatgg acgctcttga
     2161 gaaacttggt gccgccgaac aataaccaac tggggtgtag tatcgggcta tgacacgacg
     2221 agatgctgaa gggttgtcgc catctggcag gcgcgtgaag gtcagcctga acgctggatg
     2281 gctttttgag aagcagtccc gcgggattgg ggaattgggt tcctacgaac ggagaagggg
     2341 aagggaatca gagaccgagc ggatctttgt cggtgctgaa cagcttgagt acgatgactc
     2401 ttgctgggaa cgtgtggata ttccccattg ctggaacgcc catgacgtga tgacggcaca
     2461 gtcaggttat tggcgtggca tcggttggta tcgcaagacg ttccgacttg gggagcaatg
     2521 gtcagagaag cgcgtgagac tccaatttga aggtgtgaat caacgggtaa ctctctggct
     2581 caatggacga gttcttggaa gtcacaaagg gggatatacg ccttttgagt ttgacatcac
     2641 tgagcatttg aatcctctgg gtgaagtaaa tatcctggca gcccgcgtgg ataatcttta
     2701 cgacccggat atgccaccga cggtcaagac ggacatcaac ttcgttggtg ggatctaccg
     2761 tgatgtcagg cttgagggaa catatcccat ccacgtcagc tccgtatggg ttcagtcttc
     2821 aaagcttgat cacaagaagg ctgtcattgt ggccaagaca gtcgttgcca acaacagttc
     2881 agccaccaga gtagtgcagg ttcttcacaa ggtgtttgat ggggatggga acgctgtgtg
     2941 ttcattttcc ggtgaacatg aactggtcgc aaacgagctt ggtgagtttg aacatcggtt
     3001 acagactgtc agcaatccca gactctggtc gcctgagagt ccgcatctct atcgtttgtt
     3061 gactcaggtt g
//
LOCUS       DAOQUC010000331         3066 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_37162_length_3066_cov_2.643640, whole genome shotgun sequence.
ACCESSION   DAOQUC010000331 DAOQUC010000000
VERSION     DAOQUC010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3066
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37162_length_3066_cov_2.643640"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(127..>3066)
                     /locus_tag="PKH07_06875"
     CDS             complement(127..>3066)
                     /locus_tag="PKH07_06875"
                     /inference="COORDINATES: protein motif:HMM:NF028199.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HNT34702.1"
                     /translation="GGVLDKTVAKTSSESVKWSHAESEQLTLLSDSPMNWSEWSAMRF
                     WLHSEKTTGSSFMLLLFSEDENNEGIDYYSIRITLNWTGWREFLVPFREMGVTRDPVG
                     FHKIDIVRFTATGWGNVPDPEAIVHLDGFHLLQVSAQETILTDEEFLGKLDLSSPALA
                     PIRDAIAKADFIEAKRLLALHIKNRTYPKWFFDWRENPMQGVTPPPNQADISPDQWDY
                     YLHKITIDWKGWKHFRLDKAAFDTQANVERDSKVEKQGKNPIGWHWSRNRAICADGWG
                     LTPDFQTVLYFDAVRLITKTGSVSIDDFESECPKLRGLERTTQRRKQGKHSGKWALLP
                     LNTKLRCSAIPHDWTDVESLEFWLYSEEATGAEIVLILDSDLPGNNSVAENTLAKKFS
                     WNYGNRDWSIQFDEKINWAANPTTGPDKTHLWNESLNRHRFFVDVAQAYWDTGNERYA
                     EGLVQMWMDWIQSNPRPYLTSGNQAPGGNYAWQTLTTAIRIEHVWLDALYKIMISPAV
                     TGEVLFAILKSIWEQADHLMKWPSKGNWLTEECCGLFAAGMMFPEFADATAWRATAIE
                     KLYAQLDDEVYPDGMQVELAAGYNNWVVNNMTAILERAEMNQLRKELPKDFQAKIEKM
                     YDYLLSALMPNGQIPGLNDSGNTNVRQMLAKGYVLFPHRKDFLFGASAGRAGIEPTMA
                     SVALPYTGHYVMRSGWDADAVYLLFDSGPLGLGHDHEDKLHFVLFAYGKQLVLDPGNY
                     SYDRSKWRRYILDTPGHNTILVDGLGQNRRRDRANRVWPKPWDKAAPADNDTLWSSDD
                     KTDFASGIYRHGFGTDAILDVTHTRKIWSMKPDYFVIEDILTPPDDQPHTYESLFHLD
                     APSATAEIDTGVVLTENPDSANLAIVPLAVDGLTVSVVKGQEDPVQGWANHPWRAVPT
                     AIYTLKGTGTQRFVYVLYPIRKGAVCPIESLEDMVIEKDGESRKALRIRFLNGKKTIL
                     SLEGFAF"
BASE COUNT          692 a          777 c          821 g          776 t
ORIGIN      
        1 acagacgaca agactccgca agagattgct gaggttgtga tcagtttcgt gcgtgagagc
       61 atcatctgag tgcaacggtc ttgcgacgga ctctgtgaga acgcgtgaca caagacaaag
      121 accggctcag aatgcaaacc cctccagaga cagtatggtt ttcttgccat tgagaaatct
      181 tattctcagg gcttttctcg actcaccgtc cttctcaatg accatgtcct caagcgattc
      241 gatagggcac accgcgcctt tccgtattgg atagagcacg tagacaaagc gttgagtccc
      301 agtgcctttc agggtgtaga tcgccgttgg tacggcgcgc cagggatgat tcgcccaacc
      361 ctgaacgggg tcttcctgtc ccttcacgac ggacaccgtg agtccgtcaa cagccaatgg
      421 cacaatggca agatttgcag aatctggatt ctcagtgagc accacaccgg tgtcgatttc
      481 agcggttgcg gaaggtgcat ccagatggaa cagtgattcg taagtgtgag gctgatcgtc
      541 gggtggcgtc agaatatcct cgatgacaaa gtagtccggt ttcatagacc atatctttct
      601 cgtgtgtgtg acatcgagta ttgcatcggt accaaagcca tgccgataga tgccactcgc
      661 gaaatctgtc ttgtcgtcac tcgaccagag cgtatcgttg tctgccggcg cggctttgtc
      721 ccagggcttt ggccaaacac ggtttgctct gtcgcgtcgg cgattctgac cgaggccatc
      781 cacaaggatc gtgttgtggc cgggtgtgtc cagaatgtaa cgacgccatt tgcttcgatc
      841 gtatgaatag ttgccaggat cgagaaccag ttgttttcca taggcgaaga ggacgaaatg
      901 aagtttgtct tcatggtcat ggcccaatcc gagcggcccc gaatcgaaaa gcagatagac
      961 ggcgtcggca tcccagcctg agcgcataac gtaatgccca gtatagggaa gtgccacaga
     1021 agccatggtt ggctctatcc cggcacggcc tgctgatgcg ccaaagagga aatccttgcg
     1081 gtgcggaaac aggacgtagc ctttggcaag catctgtcga acgttggtgt tcccggagtc
     1141 gttcaagcct ggaatctgtc cattgggcat gagcgccgaa agaaggtagt cgtacatctt
     1201 ctcgatcttt gcctggaaat ccttcgggag ttccttgcgt agctgattca tctcagcgcg
     1261 ttcgaggata gccgtcatgt tgttgacgac ccagttgtta tatcctgcgg caagctccac
     1321 ctgcataccg tcgggataga cctcatcatc caactgagcg taaagtttct caatagcagt
     1381 ggctcgccag gcggtggcat cagcaaactc ggggaacatc atacctgctg cgaacagtcc
     1441 gcagcattcc tcggtaagcc agtttccctt cgatggccat ttcatcagat gatccgcttg
     1501 ttcccaaata gatttgagta tggcaaacaa aacttcgcct gtgacggcgg gtgagatcat
     1561 gattttgtag agtgcatcga gccaaacatg ctcgatccga atagctgtcg taagcgtctg
     1621 ccatgcatag ttgccgcccg gtgcctgatt gcccgaagtc aaataaggtc ggggattgct
     1681 ttggatccaa tccatccaca tttgcaccag tccctcagcg tatcgttcat tgcccgtgtc
     1741 ccagtatgcc tgagccacgt caacaaagaa gcgatggcgg ttcagggatt cgttccaaag
     1801 atgcgtcttg tccggaccgg ttgtggggtt ggctgcccag ttgatcttct cgtcaaactg
     1861 gatagaccag tctctgttgc catagttcca actgaatttc tttgccaggg tgttttcggc
     1921 aacactgtta tttcccggaa gatctgaatc cagaatcagc acgatttctg cacccgttgc
     1981 ctcttcagag tagagccaga actccagcga ttcgacatcc gtccaatcgt gcgggattgc
     2041 ggagcagcgc agtttggtgt tcaaaggaag aagtgcccat ttgccagagt gctttccctg
     2101 tttgcgtcgt tgtgttgttc gttccaagcc tcggagtttt gggcattcgg attcgaagtc
     2161 atcaatactt acagagcctg tcttggtgat gagacgaaca gcatcaaagt acaacactgt
     2221 ctgaaagtcc ggtgtgagtc cccacccatc tgcacagatg gcacggtttc ggctccagtg
     2281 ccatccgatg gggttcttgc cttgtttctc caccttggag tctctttcaa cgttcgcttg
     2341 agtgtcaaac gccgctttgt cgaggcgaaa gtgtttccaa cctttccagt ctatggtgat
     2401 cttgtgtagg tagtaatccc attggtcagg ggatatgtcc gcttgattgg gtggaggtgt
     2461 gacgccctgc atgggattct cgcgccagtc gaaaaaccac ttgggatagg ttctgttctt
     2521 gatatggaga gccaagaggc gcttggcttc gataaagtct gcctttgcga tggcatcgcg
     2581 aatcggcgcc aacgcgggtg aagacaagtc cagtttgcct aagaattcct catctgtgag
     2641 tatagtctct tgggcagata cctggagtag gtggaaacca tccagatgga caatcgcctc
     2701 aggatcggga acattgcccc atccggttgc ggtaaagcgc actatgtcaa ttttgtggaa
     2761 tccaacagga tctcgggtga cgcccatctc tcggaacgga acaaggaact cacgccagcc
     2821 tgtccagttg agtgtgattc gtattgagta atagtctatt ccctcattgt tctcatcttc
     2881 ggagaagagg agaagcatga aggatgagcc tgttgtcttt tcagagtgaa gccagaaccg
     2941 catggcagac cactctgacc agttcatagg actgtcagag agcaaagtga gttgctcact
     3001 ctccgcatga ctccacttga ctgattctga ggaagtcttg gcaacggttt tgtcgagaac
     3061 tccacc
//
LOCUS       DAOQUC010000332         3063 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_37198_length_3063_cov_2.890957, whole genome shotgun sequence.
ACCESSION   DAOQUC010000332 DAOQUC010000000
VERSION     DAOQUC010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3063
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37198_length_3063_cov_2.890957"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..243)
                     /locus_tag="PKH07_06880"
     CDS             complement(<1..243)
                     /locus_tag="PKH07_06880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HNT34703.1"
                     /translation="MTEVRSDIDRVLDVLHQKQRRNITHWAHLPAEPARFADLPQGLS
                     DELVVALKKRGFSKLYTHQASAFEAVRSGKDVVIVTP"
     gene            466..2700
                     /locus_tag="PKH07_06885"
     CDS             466..2700
                     /locus_tag="PKH07_06885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34704.1"
                     /translation="MNGPSSKIPAPVRWFGIFALCLGATCIFYWRIILRGEAPLFGDV
                     AVQFLPWKILIKRTLLSGHFPLWNPYSMAGKPLLANMQSAVLYPIDLLLMLFPIMRFY
                     AWSLLLHTALAGTFTWFLARRLGLSMASATLACIAFMFNGFFLIHVFAGNMLTVISSC
                     WIPAIFLAGYNLTDWSRGLSGKWFRALCATSFALSFQVLSGHPQMSFYTLFFVTLWIT
                     LLVCSQQSDIRQLVKTLCLWFAALAIAFCLCAAQLFPTLEYMRLSSRGTLSFEQATEF
                     SFSLQGLLGMVFPEFLGTHAEGTFWHRWYYWSCAYMSTLLFLLALVALPLARRMGVER
                     TQRRRFFVPLWCVVAVSVFLALGRQNPLYHLVFQLPGFSSFRAPAKFLPFAVLGLCLL
                     GARGLDVLLTLLSAPKTSDRVTDRHLVQGLALVIGVILLGSLLIWPFRAIPEVQQAQT
                     RFTVAASRSLTNTAFHLLCAVVILLGVWRRYWSKPAAVGSLLVLVFTDLWNFGLKYQT
                     FSRPEGLYEETNEIRYLYPETWEDYWRIATMNDVPYPEAFAVFRKENLCGYDPLSLGT
                     YAEMFKVSQGQSPSDFRDEFNLERISDALLDAFNVKYVLTRRTLDSPTMKRVFSGTRF
                     DVYERANCPPRVQGEGAQIRWKRPKFNEYRFSVYAASETVVRIAQLAYPGWRAMLNDT
                     PTEISADEQGFMTLRVPEGRSEVWIFYRPQSFLMGLWISVAALALMSICLLAAAVGRS
                     QPKH"
     gene            complement(2743..>3063)
                     /locus_tag="PKH07_06890"
     CDS             complement(2743..>3063)
                     /locus_tag="PKH07_06890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34705.1"
                     /translation="GFFIQTEAPGFYRVSLPTSMKDAEVSPSVFCVNLDTVESDLTTF
                     ERKSLDAALAGRFKVEYLQPSPFVGERLAKAREGVGLWNYLLLLLWGVVMAETIYANR
                     LTQS"
BASE COUNT          641 a          822 c          799 g          801 t
ORIGIN      
        1 cggcgtaacg atcaccacgt ccttgcccga acgcaccgct tcaaaggcgg acgcctgatg
       61 tgtatagagt ttggagaaac cgcgcttctt tagagcgaca acaagttcgt ccgacaaacc
      121 ctggggaaga tcggcaaaac gagctggttc cgctggaaga tgcgcccagt gggtgatatt
      181 gcgtcgctgc ttctgatgca acacgtccag aacgcgatca atgtcagacc gtacttccgt
      241 catatctcaa cgtaacctct ttcaacctac ggcagccagg atgcgcaacc ccgcgcactc
      301 acaacacgta acccttcctt gcctgcgctg gatcgtccga acggcaggaa tgatatcatt
      361 ctgcatgaaa ccactcaagc gatcatgcga atggctctgt attgttctgt gtttatgagt
      421 tattgtaaat cctattgttt tgcagaccac aaaggtaaga aagacatgaa cggcccttcc
      481 agtaagatac cagcacctgt tcggtggttc gggatattcg cgttgtgcct cggtgcgacc
      541 tgcattttct actggcgaat catccttcgc ggtgaagcgc cgctgtttgg cgatgttgcg
      601 gttcagtttc tcccgtggaa gatattgatc aagcgcacgc tcctgagtgg acatttcccc
      661 ctgtggaatc cttatagcat ggcgggaaag cctcttctgg cgaacatgca gtcggcggtg
      721 ctctacccca tagatctcct tctgatgctg tttccgatca tgcgtttcta tgcctggagc
      781 ctgcttcttc acacggcgct ggcgggcacg ttcacatggt ttctggccag acgactcggg
      841 ctttcgatgg cttcggcgac gctggcttgc attgcattca tgttcaacgg ctttttcctg
      901 atccacgtct ttgcggggaa catgctcacg gtcatctcca gctgctggat tccggcaatc
      961 ttccttgcgg gatacaacct gacagactgg agtcgtggcc tgtcaggcaa gtggtttcgc
     1021 gccttgtgtg ccacctcatt tgccttgtcc tttcaggtat tgtccggtca tcctcaaatg
     1081 tctttctaca ccctcttttt tgtcacgctc tggatcactc tgctcgtctg ctcacagcaa
     1141 tctgacatca ggcaactggt caaaacactt tgtctgtggt ttgctgcctt ggcgattgcc
     1201 ttctgtctgt gcgcggcgca actttttcca actcttgagt acatgcgtct ttcaagtcgg
     1261 ggaacactct ccttcgaaca ggccacagag ttctcttttt ccctgcaggg acttctcggc
     1321 atggtctttc cagaattcct gggaacacat gccgaaggaa ctttctggca tcggtggtac
     1381 tactggtctt gtgcgtacat gagtacgctc ctgtttctgc tcgcactcgt tgcgctgcct
     1441 ctcgcgagaa ggatgggggt tgaacgcacg caacggcgaa ggttctttgt tccgctttgg
     1501 tgtgtcgtcg ctgtctctgt gttcctggcg cttggaagac agaatccgct ctatcacttg
     1561 gtatttcagc ttccagggtt ttcatccttt cgcgcacctg ccaagtttct gccgtttgcg
     1621 gtgctgggct tatgccttct cggagcgcga ggtctggacg tgcttctgac tttgttgagt
     1681 gcgcccaaga cctctgatcg agtcacagat cggcatcttg tgcaaggact cgctctcgtg
     1741 atcggtgtca ttctcttagg ttcccttttg atctggccat tcagagcgat tcctgaggtt
     1801 cagcaagcac aaacgcgatt cacggtagcg gcgagtcgct ccttgacgaa cacggcattc
     1861 catctgcttt gtgcggtcgt gattttgctc ggagtttggc gaagatattg gagcaaacct
     1921 gcggctgttg gatcactgct agtattggtt ttcacagact tgtggaactt cggtttgaag
     1981 tatcaaactt tctccagacc cgaaggtctc tatgaggaga ccaacgaaat tcgatatctt
     2041 taccctgaaa cgtgggagga ttattggcgc atcgccacaa tgaatgatgt tccctacccc
     2101 gaagcgttcg ctgtctttcg gaaagagaac ctctgcggtt atgatcccct cagcctcggt
     2161 acctatgcgg agatgttcaa ggtttcacaa ggtcagtctc catctgactt tcgcgatgag
     2221 ttcaaccttg agaggatttc ggatgccctg ttggatgcat tcaacgtcaa gtatgttctg
     2281 acgcgacgga cactggacag ccccaccatg aaacgggttt tcagtgggac acgttttgat
     2341 gtctatgagc gagcgaattg tccgccaaga gttcagggag aaggcgctca gatcaggtgg
     2401 aaaaggccaa agttcaacga ataccgattc tctgtctacg ctgcgagtga gacggtcgtg
     2461 cgcatcgcac aactcgccta ccctggatgg agagcgatgc tcaacgacac accaaccgag
     2521 atatctgccg atgagcaagg attcatgaca ctccgagttc cagagggacg gagtgaagtt
     2581 tggatattct acagaccgca gtcgttcctg atgggtctat ggatcagtgt tgcagcactg
     2641 gcgctgatgt ccatctgcct tcttgcggcc gccgtaggac gtagccaacc caagcactga
     2701 aggtcatcaa aacactcggg gggtctcaat tggaaacgca tactacgact gggtaagccg
     2761 attggcgtag atcgtctcag ccatcaccac gccccagagc agcaagagaa gatagttcca
     2821 cagtcctacg ccttcgcgag ccttggccaa acgctcgccc acaaagggtg atggttgcag
     2881 gtactcgact ttgaagcggc cagcaagcgc agcatccagg ctctttcgct caaatgtcgt
     2941 cagatcggat tctacggtat ccaagttcac gcagaacaca gagggtgaga cctcagcatc
     3001 cttcattgag gtgggcaaag agacccgata gaatccgggt gcttcggtct ggatgaagaa
     3061 acc
//
LOCUS       DAOQUC010000333         3062 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_37211_length_3062_cov_3.198204, whole genome shotgun sequence.
ACCESSION   DAOQUC010000333 DAOQUC010000000
VERSION     DAOQUC010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3062
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37211_length_3062_cov_3.198204"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1155)
                     /locus_tag="PKH07_06895"
     CDS             complement(<1..1155)
                     /locus_tag="PKH07_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HNT34706.1"
                     /translation="MSKVNLTPEEKDRERRLKWFRAARFGMFIHWGLYSQLGRHEWVM
                     NRERIPIPEYEKLASTWKPKRNAPRVWARLAKEAGMRYMVMTTKHHEGFCLFDSKLTD
                     YNAAQRGPGRDLVSEYVEAARAEGLRVGFYYSLMDWHHPDGARCIKDERARRRFVDYI
                     HGHVRELCTNYGKVDILWYDVGWPLSAEGWESAKMNAMVRQLQPDIIINNRSKVPEDF
                     GTPEQHITPEKSGRMWEACMTFNQSWGYTPIDSRYKDAWDVISMLRQVAAGGGNLLLN
                     IGPTPEGNVPSVCSKVLKQVGPWLQKHGDSIYQAIDPMHSEWMCTGAFTRKGDIAYYH
                     CNQWPGRQLAIGGLMNKVEDVRLMGGARVKFAQTKDRLVISGLPEKAPDPI"
     gene            1384..2316
                     /locus_tag="PKH07_06900"
     CDS             1384..2316
                     /locus_tag="PKH07_06900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34707.1"
                     /translation="MRLALTVTLVLALAPIVQAGTWIDNFEDGALWTEWQVFEGAEKV
                     DAPSIEEADGRATLSVQPGQTLTLSAGQFEGDFFFTLRSGLPSITAKGKIGLFLGKAP
                     LTPGTPAVHASLVHSPDSPEFRAGVVGETESNKPYMAISSFAVVLSRAGEQVTVFIGE
                     DDTEPLDQLGQAFALPAGPVDAFIALSADADAEPWKIQIDEVKITGDSVPDKAPATVA
                     AATEATGEGKPFDPNEASRHANSGFKMLQRGQFEEALNEYAIAAQLSKRYVEIHNTLR
                     DHIVPALRSPGGREAIRDVPIGPKGTTIGEWLQW"
     gene            2418..>3062
                     /locus_tag="PKH07_06905"
     CDS             2418..>3062
                     /locus_tag="PKH07_06905"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNT34708.1"
                     /translation="MRTSALLKIGSKRILLDPCVDFRQQALRFSVDHVDAVLVTHPHA
                     DHIFGLDELRIYSQRTNAPISVYGSRATIEEIKVTFRYVFRPPQMGGGVPQLDLHIVQ
                     GSFLVCGLPFQTIPIKHGVLDIEGYRIGDVAYLTDCSEIPQTSWPLLEGVQTLVLGAL
                     RFRPHPTHFSLDQALAVVARLKPKNTFFVHICHDMDHDSVCEQLPPGVSLAYDGL"
BASE COUNT          681 a          822 c          769 g          790 t
ORIGIN      
        1 aatggggtca ggcgcttttt ccggcagtcc actgatcacg agacgatcct tggtttgggc
       61 aaacttgacc ctcgccccac ccatcaatcg cacatcctca accttgttca tcaaaccgcc
      121 gatggcaagt tgccggcctg gccattgatt gcagtgataa taggcgatgt cgccctttcg
      181 agtaaatgct ccggtacaca tccactccga gtgcatcgga tcgattgcct gatagatgga
      241 atcaccgtgc ttctgaagcc agggaccaac ctgtttgagg actttggagc agactgatgg
      301 aacatttccc tctggagtag gcccgatatt caggagcaag ttgccaccac ccgccgcgac
      361 ctgccgaagc atggatatca catcccaggc atccttgtac ctgctatcaa ttggagtgta
      421 accccaactc tggttgaaag tcatgcaggc ttcccacatt cgcccagatt tctccggcgt
      481 aatatgctgt tctggagtac caaaatcctc tggaaccttc gagcggttgt taatgatgat
      541 atcaggctgt aactggcgaa ccatcgcgtt cattttagcg gattcccagc cctccgcact
      601 gagcggccag cccacgtcat accagagaat gtcaaccttg ccatagttgg tgcagagttc
      661 cctgacatgg ccgtgaatat agtccacaaa acgacgcctg gctctttcgt ccttgatgca
      721 tctggcgccg tcgggatgat gccagtccat cagtgaatag tagaacccaa cgcgaagccc
      781 ttcagctcga gccgcttcaa catactctga gacaagatcc cggcctgggc cgcgctgcgc
      841 tgcattgtag tcggtcagct tcgagtcaaa aagacagaaa ccttcatggt gcttcgttgt
      901 cataaccatg tagcgcattc cggcttcttt cgccagtctt gcccaaacgc gaggagcgtt
      961 tcttttcggc ttccacgtgg aagccaattt ttcatactca ggaattggga ttcgctctcg
     1021 gttcatcacc cactcatgac gtcccaactg ggaatagagt ccccaatgaa tgaacatccc
     1081 aaaccgggct gcacgaaacc atttcagtct tcgttctcta tccttttcct ctggagtcag
     1141 atttactttg gacatccctg catctccctt ctagctcacc aggcgtcagt tcggtttgcg
     1201 ctctccactg ccgattcgca gcagaattga tacctatgtt ggtataagtt atttgttccg
     1261 atgtcaagtg tcatgttatc caaggtgttt atccttcaga atttcttgtc tctcccttca
     1321 ttttcatcta gaatgccttt ggttgatgag aagcccatca gaaacgaatg gaggaaggca
     1381 gcgatgagac tggcattgac tgtgacctta gtacttgcgt tggctcccat cgttcaggca
     1441 ggtacgtgga ttgataattt tgaggacggt gcgttgtgga ctgaatggca ggttttcgaa
     1501 ggagccgaga aggtggatgc accctcaatt gaggaagcag acggcagagc gaccctctca
     1561 gtccaacccg gccaaaccct gactctatcg gcaggtcaat tcgaaggcga tttcttcttc
     1621 actcttcggt ccggcctgcc gtcaattacc gccaaaggaa agatcggttt gtttctcggg
     1681 aaagcacccc tgacgcctgg cacgccagct gttcatgcat cgctggtaca ctcacccgat
     1741 tctccggaat ttcgtgcagg ggttgttggt gagactgaga gcaacaagcc ctatatggcc
     1801 atcagctctt tcgcagttgt gctgtctaga gcgggcgaac aggttactgt cttcattgga
     1861 gaagatgata cggaaccact tgatcaactt ggccaagctt ttgcgttgcc tgccggccca
     1921 gtagatgcgt tcatcgccct ttccgcggac gcggatgctg agccgtggaa aatccagatt
     1981 gacgaagtga agatcacggg agacagtgtt cccgacaagg cacctgcaac tgtagccgct
     2041 gcaaccgaag cgacaggcga aggaaaacca ttcgatccga atgaggcctc gcgacatgcc
     2101 aattccggct tcaagatgct gcaacgggga caatttgaag aagcactgaa tgagtatgcc
     2161 attgcagctc agctgagcaa gcggtatgtt gagatccaca acaccctgcg agatcatatc
     2221 gttcccgcgc tccgctctcc aggtggacgc gaagcgattc gagacgtgcc gattggacct
     2281 aagggaacta ccatcggcga atggcttcag tggtgagcga tagtcttctc tttcttggga
     2341 ctgggacgtc tagcggtgtt ccggttattg gatgtttttg tgatgtctgt tgttcttcaa
     2401 atccaaagaa caatcggttg agaacttctg cattgctcaa aatcgggtcg aagagaattc
     2461 tgcttgatcc ctgtgttgat tttcgtcaac aggcacttcg gttctcagtt gatcatgttg
     2521 atgctgtctt agtgactcac ccccacgcag accatatctt tggactggat gagttgcgaa
     2581 tctactcaca gcgcacaaac gcaccgattt ccgtctacgg ttcgagagca actattgagg
     2641 agatcaaagt cactttccgt tacgttttca ggccgccaca gatgggtggt ggagttcccc
     2701 agctagacct gcacattgtt cagggttcct ttcttgtctg cggactcccc tttcagacga
     2761 tcccgatcaa gcatggtgtc cttgacatcg agggatatcg cattggcgat gttgcttatc
     2821 tgacagactg cagtgagatc ccccaaacgt cgtggcctct gcttgaggga gtgcagactt
     2881 tggtcttggg tgcgttacgg ttccgtccac atcccacgca tttcagtctc gaccaggcac
     2941 tggcagtggt ggctcgtctc aagccgaaga acacgttctt tgtccatatt tgccacgaca
     3001 tggatcacga ttcggtatgt gagcaacttc cacccggtgt gtctcttgca tatgatggct
     3061 tg
//
LOCUS       DAOQUC010000334         3058 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_37284_length_3058_cov_3.025308, whole genome shotgun sequence.
ACCESSION   DAOQUC010000334 DAOQUC010000000
VERSION     DAOQUC010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3058
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37284_length_3058_cov_3.025308"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1108
                     /locus_tag="PKH07_06910"
     CDS             <1..1108
                     /locus_tag="PKH07_06910"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNT34709.1"
                     /translation="SMKILHIYKDYYPPVLGGIETHINALATGMAHRHEISVLVCGKG
                     WQTTRENLDGVTVVRIGCPIRAFSNPFAPCFPYWLRRLDADILHFHLPLPTATVAWQI
                     ARPKGKVIVTYHSDIVRQSWALAAYGPLQQAFLRKAFRILVTSPNYLQSSTHLQSHRE
                     RCRVVPLGMDLALFEPNEAVIRHATMLREQWGSRVVLFVGKLRYYKGLTYLISAMKSV
                     NATLVLVGGGPLETQLRRQAQEEGISERVVFVGDMERDQLPSYYYACDLFCLPSIYRS
                     EAFGLVQIEAAACAKPVVSTALASGVPYVNQHEKTGLIVPPQNPEALAQAINTLLDDP
                     ALSQRMGSYARERAFREFTLEKMLERVESIYEEC"
     gene            1274..2818
                     /locus_tag="PKH07_06915"
     CDS             1274..2818
                     /locus_tag="PKH07_06915"
                     /inference="COORDINATES: protein motif:HMM:NF022623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HNT34710.1"
                     /translation="MKCALAVVCLVLPFCSPSFAQFDLRQLSELTPGVVRTENSLWPE
                     NPIEVRFDSPSEEVVLADLKGPGEITMIHFAMPESSILRPTEYTFGRDLVMQIYWDGE
                     KTPSVDCPVVDFFCDPAGLRDIVNTALVNKCRGFNAYFPMPFRKSARVILRYDGEEPP
                     SWDRWRLLPCYGYVMHRSGIKISSRQGYFHAQFRQQALLLGKEDYVALEAQGRGKFVG
                     WNVTVRRPGTVGYPVDMNEKFFVDGEDTASTEFQGLEDSFGFSYGFPPAENFFPFTGF
                     FPFTNEGAAAYRFFVNDSISFKKSLRVTIGFGVNENPMFRQSFSAWGTSLQFSSVCYW
                     YQTEPHAPFPKMLSAEDRAPAPSQLFWPDQENLPSEDDLRSQSVQLEMWCGRPDPEVI
                     YAEPGYSAQTRQGESWCGWNAPTYHCRSHREELIIDLTVPSQASGIVRVYIKDPDNYM
                     GGRSQRVLVGDKDLGEHSQFGEGRWLEVPVTAEMTSQGIVTVRAMNLNPASNAVISNI
                     QWLRAP"
     gene            complement(2830..>3058)
                     /gene="rbsC"
                     /locus_tag="PKH07_06920"
     CDS             complement(2830..>3058)
                     /gene="rbsC"
                     /locus_tag="PKH07_06920"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="functions to transport ribose at high affinity;
                     forms a complex with RbsA2C2B; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HNT34711.1"
                     /translation="GTGLELEVITAVVLGGTSITGGRGTILGTFLGVLILGFARNGLN
                     LAGISSVWQAILAGSLLILTAIVNNRLASRR"
BASE COUNT          688 a          848 c          797 g          725 t
ORIGIN      
        1 ttctatgaag atactgcata tctataaaga ctattaccca cccgttttgg gtggtatcga
       61 gacccatatc aacgcgctgg ctacaggaat ggctcaccgt cacgagatct cggttctcgt
      121 ttgtggcaaa ggatggcaga cgacacggga gaatctcgat ggggtcactg tagtccgaat
      181 cggctgtccc attcgagcct tttcaaatcc ttttgccccg tgttttccct actggctgag
      241 aagactggat gcagacattc tacacttcca tcttcctcta ccgacggcaa cggtcgcctg
      301 gcaaatcgcc cgccccaagg gcaaggtcat cgtgacgtat cacagtgaca ttgtcagaca
      361 atcatgggca cttgcagcct atggccccct gcagcaggca ttcttgcgca aggcctttcg
      421 tatcctcgtc acatctccca actacctgca atcctcaacg catctccaat cacatcgaga
      481 gcgctgtcgc gttgtcccgc ttgggatgga tttggcactc ttcgagccaa atgaagccgt
      541 tattcgccac gccacaatgc ttcgagaaca atggggatcg cgagtggtgc tgttcgtcgg
      601 aaagctccgg tactacaaag gtttgaccta tctcatcagc gccatgaagt ccgtcaacgc
      661 aaccctggtt ctcgttggcg gaggcccctt ggaaacacag ttgcggagac aggctcagga
      721 agaggggatt tccgagcgtg ttgtctttgt gggagacatg gaacgcgacc agttgccgtc
      781 atactactat gcgtgtgatc tcttctgtct gccttcgata taccgaagcg aggcatttgg
      841 tctcgtacaa attgaagccg ccgcgtgtgc caagccggtg gtaagcaccg ccttggcttc
      901 cggcgtacct tacgtcaacc agcacgagaa gaccgggctt attgtgcccc cccagaatcc
      961 agaagcgctc gcacaagcga tcaacactct ccttgacgat cccgccctgt cgcagcggat
     1021 gggttcatac gctcgggagc gagcatttcg ggaattcaca cttgagaaga tgttggaacg
     1081 ggttgaatcc atctatgagg aatgctgagt ggcaaccagg cgatacgttt ttctgcagag
     1141 ggcatagagg cgaggagaga tgaaaccaaa gacatcccgt ctccatctcc tcttgcgtga
     1201 atgacgccag ttgtcccctg cgatgaggtt ttcctatgtt cagagtacct tttgatccag
     1261 gagttgaact accatgaaat gtgcattggc tgtcgtctgt ctcgtgttgc ctttctgttc
     1321 accctctttc gctcagttcg acctgcggca actctctgag ctgacacccg gtgttgttcg
     1381 aactgagaac tccctctggc ctgaaaatcc catcgaagtg agatttgact ctcccagcga
     1441 agaagtcgtt ctcgccgatc tgaaaggtcc tggcgagatc acgatgattc acttcgcgat
     1501 gccggagagt tccattcttc gaccaactga atacacgttt ggacgagatt tagtgatgca
     1561 gatctactgg gatggtgaaa agactccgag cgttgactgc cctgtggtgg atttcttctg
     1621 cgatccggca gggcttcgag acattgtcaa caccgccttg gtaaacaagt gtcgtggttt
     1681 caacgcttat ttcccgatgc cgtttcgaaa gtccgcacgt gtcattctgc gctacgacgg
     1741 agaagaaccg ccgagttggg atcgctggag acttctgccg tgctatggct acgtcatgca
     1801 ccgcagcggc atcaagatct cgtccaggca gggctacttt cacgctcagt tccgtcaaca
     1861 ggcactcctt ctgggtaaag aagattatgt ggctctggaa gcgcaggggc ggggcaaatt
     1921 cgtcggctgg aacgtcaccg tccgaaggcc tggcactgtc ggctatcccg ttgatatgaa
     1981 tgagaagttc tttgtagatg gcgaggacac agcttccaca gagttccaag gtctggaaga
     2041 ttccttcggt ttcagttacg gatttccgcc agcggagaat ttctttccct tcacaggctt
     2101 cttccctttc acaaacgaag gagccgcggc ctatcggttc tttgtcaacg attcgatcag
     2161 tttcaagaag tctcttcgcg tcaccatcgg attcggcgtc aatgaaaacc cgatgtttcg
     2221 tcagagtttc agtgcctggg gcacctcgct tcagttctca agcgtctgct attggtatca
     2281 aacagagcct catgcaccgt ttcccaagat gctgtccgcc gaggatcgtg ctcccgcgcc
     2341 gtctcagctg ttctggcctg accaggagaa tctaccctct gaagacgacc tgcgctccca
     2401 aagcgttcag ttggagatgt ggtgtggcag accagatcca gaggtcatct acgcagaacc
     2461 aggatactcc gcccagacca gacaaggtga atcctggtgc ggatggaatg cgccaaccta
     2521 tcactgccgc tctcatcggg aagaactcat cattgatctg acggttccca gccaagcaag
     2581 tggcattgtc cgagtctaca ttaaggatcc ggacaactat atgggcggac gcagtcagag
     2641 agttctcgtc ggagacaaag acctgggcga gcacagtcag ttcggtgaag gtcgttggct
     2701 tgaggttcca gttacagccg aaatgacttc ccaaggcatc gtaactgtgc gggcaatgaa
     2761 tttgaatcct gcttctaatg cagtcatctc aaacatccaa tggttgcggg cgccctgaca
     2821 ctcccgaaat caccggcggg atgcaagacg gttattcacg atggcggtca agatgagcag
     2881 ggaacccgcg aggattgcct gccagacaga tgaaatgccc gcaaggttca aaccatttcg
     2941 cgcaaaaccc agaatcagca cgccgagaaa cgtcccaagg atcgttcccc gtcctccggt
     3001 gatcgaggtg ccacccagaa caaccgccgt gatcacttcc agttccagac ccgtccct
//
LOCUS       DAOQUC010000335         3057 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_37312_length_3057_cov_2.893738, whole genome shotgun sequence.
ACCESSION   DAOQUC010000335 DAOQUC010000000
VERSION     DAOQUC010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3057
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37312_length_3057_cov_2.893738"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..699)
                     /locus_tag="PKH07_06925"
     CDS             complement(<1..699)
                     /locus_tag="PKH07_06925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34712.1"
                     /translation="MKSRERLLRALNCERPDRVPISTYELVGYNSRSFENNQPSYKSL
                     MDFIRQETDCIAMWNPACNAHFVLSAYPVEVETDQKRDGDTTTIRQVFHTPQGDLTCT
                     SKRLDGVNTTWRVERLCKNTTDVDRALLVPYVPPTYDASDYARIKSEVGNHGIIMASL
                     ADPLCHAAELMEFGEYTIWAMTEPDHFTKTLQALHERVMDNLTRMLEVNVVDLYRICG
                     PEYATPPYLPPECFA"
     gene            complement(725..2911)
                     /locus_tag="PKH07_06930"
     CDS             complement(725..2911)
                     /locus_tag="PKH07_06930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34713.1"
                     /translation="MGCSSKFRVCMNLLTIGVLATCSSLAFSQIKDSSLASMRRDLVT
                     LFRTQTSQPSLAYHGEMATRLEPILVQQSRYLVSLLRPWEQDEHALLLTKGGSGEHDI
                     RPNAHTAAGLATIYRCLPREQFPADFTPEFCRDRAIDILRFLLLTHGAGGKRCADGKN
                     WRGQWQSAHWAASTGTACWLLWDELSEDMRWLAAKMITEEADRFVGVIPPAQVKNDTK
                     AEENAWNSEVISLAFNMFPDHPHHDIWGETAIRWAVSSFARQQDVTCDMLCDGRPLRD
                     WLTAPNIHDDYTLENHSRVHPDYMNTIDMLLMQIRFYEWADRPHPEALEHNVRGIYEN
                     LKVLSFADGGYLYPNGQDWHLHRCPDWFRTHVSQAVLYGDRQAARLARICLETIECMA
                     ARNPDGALFLQDEFFFASTQHMFFELACQAYLMLRTKGEGAPPISEKELICQLAGTYL
                     FESGEFAILRTPNSVSTFSWGARTMGMVLPLQKDILLTPYESSLVGTVIIDSVRHERS
                     VVQEVRYIEMERGFAVVGVLERAEGAVKQRFGFAALRDDRCLYADRLYSRSEVHIDAL
                     ELGTIGIANEPHWVHHDGVRTLHFGRGREYITGDGLDETPFLFSHPWFNIDNVMGIVS
                     LAGSGNQTWTPLHRYQRGRLQQLFVLNAYDDRSALIREEDGLVAETAIVFYPGQSQRK
                     TRQAASRCRYQALPDDRFAICLEDGVRVQFDLQRLTVNVLPASRQN"
BASE COUNT          727 a          812 c          805 g          713 t
ORIGIN      
        1 tgcgaagcat tccggcggca gatagggtgg cgttgcgtat tccggaccgc atatccgata
       61 gagatcaaca acgttgacct caagcatacg ggtgaggttg tccattacgc gctcatgaag
      121 agcctgcagg gtcttggtga aatgatccgg ctcggtcatt gcccagatgg tatattcacc
      181 aaactccatc aactcagccg catgacagag agggtcggcc agagaagcca tgatgatgcc
      241 gtgattgccg acctcagact tgattcgcgc gtagtcagac gcatcgtaag ttggggggac
      301 atagggtacg agtagagccc gatccacatc tgttgtgttc ttgcacagtc gctcgacacg
      361 ccatgtggta ttgacaccat caagccgttt ggacgtgcac gtcagatctc cttgaggagt
      421 atgaaagacc tgtctgatcg tggtggtatc cccgtcgcgt ttctgatcgg tctcgacttc
      481 caccggatag gccgacaaca caaaatgagc attgcaggcg ggattccaca tcgcgatgca
      541 atcggtctcc tgtcggataa aatccatcag cgatttgtag gagggctgat tgttctcaaa
      601 tgatctgctg ttgtacccga ccagttcata cgttgagatg ggtacacgat ccggtctctc
      661 gcaattcaat gctctcagaa gccgttctct cgatttcatg aaccgtattc ctcatttacc
      721 aaaatcagtt ctgccgactt gctggcagga cgttgactgt gagtctctgc aagtcgaact
      781 gaactcggac tccatcttca aggcaaattg cgaaccgatc atctggcagc gcctgatatc
      841 ggcaacggga cgcagcctgt cgcgtttttc gctgagactg acctggataa aagacaatgg
      901 ctgtctcggc aacaagcccg tcttcctcgc gtatcaaggc actgcgatcg tcataggcgt
      961 tcaagacaaa cagctgctga agcctgccac gctgataccg gtgcagaggt gtccacgttt
     1021 ggttgcctga tccggcgaga ctgacaattc ccatcacgtt gtcaatgttg aaccaaggat
     1081 gggagaacaa gaaaggcgtt tcatccagac cgtcgccggt gatgtactca cgccctcttc
     1141 caaagtgcaa agttcggaca ccatcatgat gaacccaatg cggttcattc gcgattccga
     1201 tcgttccaag ttccagggcg tctatatgga cttctgaacg tgagtagagg cggtctgcat
     1261 agaggcaacg atcgtctcta agtgccgcaa atccgaatcg ctgctttact gctccctctg
     1321 ctcgttccaa gaccccgacg acagcgaagc ctctttccat ctcaatgtag cggacctctt
     1381 gcaccacaga acgctcatgg cgcacagagt caatgatgac tgttcctacg aggctcgatt
     1441 catacggtgt gagcagaata tccttttgaa gaggcaaaac catgcccatg gttctcgctc
     1501 cccaggagaa tgtcgagacg gagtttggcg ttctcaggat ggcaaactct cccgactcaa
     1561 agagataggt tccagccagt tgacaaatga gttccttctc gctgatgggg ggagcgcctt
     1621 ctcctttcgt ccgcaacatc agatacgcct ggcacgccag ttcaaagaac atgtgttgtg
     1681 tcgaggcaaa gaagaactcg tcctgcagaa acaaggctcc atcggggttt cgggcggcca
     1741 tgcactcgat ggtttcaagg cagatacgtg ccaggcgagc ggcctggcgg tcgccataga
     1801 gaactgcctg acttacatgc gttcgaaacc agtctgggca gcggtgcaga tgccagtctt
     1861 gaccgttcgg atagagatat ccgccatcgg caaaactcag cactttcagg ttctcataga
     1921 ttccgcgtac attgtgctcc agcgcttcag gatggggacg atctgcccat tcatagaagc
     1981 gaatctgcat gaggagcatg tcaatggtat tcatgtagtc cggatggacg cgggaatggt
     2041 tctccaatgt gtagtcgtca tggatgtttg gtgccgtcag ccaatcgcgg agcggacgac
     2101 catcacagag catatcgcag gtcacatcct gctggcgcgc aaaagatgat acagcccagc
     2161 gtatggccgt ctctccccag atgtcatgat ggggatgatc gggaaacatg ttgaatgcaa
     2221 gagagatcac ttccgagttc caggcgtttt cttccgcttt ggtgtcgttc ttgacttgag
     2281 cgggaggaat cactccaaca aagcggtccg cctcttccgt gatcatcttt gctgcgagcc
     2341 aacgcatgtc ttcggacaac tcgtcccaaa gaagccagca cgcggttcca gtactggcag
     2401 cccagtgtgc cgactgccac tggccgcgcc agttcttccc atcagcgcac cgtttgccac
     2461 cggctccatg cgtgagaagc agaaaacgca ggatgtctat ggcacgatcg cggcaaaact
     2521 ctggggtgaa atcggcaggg aactgctctc tgggcaggca tcggtagatc gtcgccaaac
     2581 cagccgcggt gtgggcgttc ggtctgatgt catgttctcc cgagcctccc ttggtcagca
     2641 gaagagcatg ctcatcttgt tcccagggtc tgagcagtga gaccagatat cgcgattgtt
     2701 gaacaagtat cggctccagg cgagtggcca tctcgccatg atatgcaaga ctcggttgag
     2761 aggtctgtgt tcggaacaga gtgacaagat cccttcgcat agaagccagc gaagagtctt
     2821 tgatttgtga gaacgcaagg gaactgcatg ttgccaaaac gcctatcgtc aagagattca
     2881 tacagactct gaatttggat gaacaaccca ttcggaagac accttcccgt ttctttcaga
     2941 tcaactcaaa cagcaagcaa gaatatccaa gactcacctt gtacgcaacg agcaatccgt
     3001 ttttttactg cagattcttc ttgagacagc atcggtagaa tttcagaacc aatctta
//
LOCUS       DAOQUC010000336         3050 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_37452_length_3050_cov_3.142905, whole genome shotgun sequence.
ACCESSION   DAOQUC010000336 DAOQUC010000000
VERSION     DAOQUC010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3050
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37452_length_3050_cov_3.142905"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..767)
                     /locus_tag="PKH07_06935"
     CDS             complement(<1..767)
                     /locus_tag="PKH07_06935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34714.1"
                     /translation="MSMRTMPTARLFLALLALAVVMMPATRGQAEPVGCMGGAAEPAI
                     ALKGAHPGLEDLEKAFASAAKEFGVPVEILEAVGYKESAWVQAGPTIDYGYGIMHLVE
                     GKTLQEAAELLGCSIEDLKSDALLNIRGGAALIAKYAKETVGKAKSLEDYWDALKKFT
                     ALEGDQIQEIQTEEYFEIINNGVTAENGLGMKIALSGTPVDVSAKVTKDKFVQSTDYA
                     PAVWIAARNYSSRTSGIDRWVNHWVGVGTFAGAVSWF"
     assembly_gap    1379..1478
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1741..>3050
                     /locus_tag="PKH07_06940"
     CDS             1741..>3050
                     /locus_tag="PKH07_06940"
                     /inference="COORDINATES: protein motif:HMM:NF022481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HNT34715.1"
                     /translation="MKGAAAGILLFWGIFAGVMSVYLLTLCPSIYWGDSAEFVTVVHT
                     LGVPHPTGYPLYCIFGKIFDLLPFGNPASRLNLFSALAASLGVAVVFLIVRASLLSAP
                     ELSSRSLAMAFWAAACLAFTQLYWSQAIIAEVYSLSALLIALLILVSFRNWHVPQRSY
                     EIAFGLLAGLCLTHHAQSVFVIMPLGLLMIWRMGFSTGYMRRWLAMGVCAIVPLGLYL
                     YLPLRSRANPPIDWGNPETLANFLWMISGGQFKMFLWKGLTGSSSLLAYWSRMTTFYA
                     TQFWSQWHLLSLLIPFGIRRLWKHDRAPCFALVVAWVALVFHALNYQVGDLEVYFLPT
                     YLIAVVFMAHGLFDILEWLGQRASPSYTKALNAAVLCIPLAFLTANLPYVSLSGFRAA
                     EDYGVSVFEQVQAPAVIITHSDNDIYPLWYQKYVCGLGQDVAVVG"
BASE COUNT          611 a          818 c          729 g          792 t
ORIGIN      
        1 tggaaccaac tgaccgcacc tgcaaaagtt ccaacgccaa cccagtggtt gacccaacgg
       61 tcaatacctg acgttcggct gctgtagttc cgagcggcaa tccatacagc cggagcgtag
      121 tccgtagact gaacgaactt gtccttggtc accttggcag agacatccac aggagtgcca
      181 gacagtgcga ttttcattcc cagaccattc tccgctgtaa caccattgtt gatgatttca
      241 aagtactctt cggtctggat ctcctgaatc tgatcccctt caagggcagt gaatttcttc
      301 agtgcatccc aataatcttc aaggctcttg gccttcccaa cagtctcctt tgcatacttg
      361 gcgatcagag cagcgccacc gcggatattc agcagtgcgt cactctttag atcttcaatg
      421 gagcaaccga gcaattcggc cgcttcttga agagtctttc cctcaaccaa gtgcatgatt
      481 ccgtagccgt aatcaatggt aggcccggcc tgaacccagg cggactcttt gtagcccacg
      541 gcctccagaa tctcaacagg cacaccgaac tccttggctg cactggcaaa agccttctcg
      601 aggtcttcca agccggggtg tgctcctttc agagcaatgg ctggctcagc agcgcctccc
      661 atgcaaccga ccggttcggc ttgtccacgg gtcgccggca tcatcacaac agcaagtgcc
      721 agcaacgcca ggaacagacg tgccgtcggc atcgttcgca tactcattct tttcctcctg
      781 ctacggtgat cccatcggat cactcatcaa ggatttcatg cttccctcac aagctcattg
      841 cgttgatcac agtgcatctc ataggaaacc tcagcaacgc aaagcttgtt ccgataggtg
      901 tcgaaactct ttgggcatcg cctcctttcc caagaaaaat gaataccatc ctgctagaga
      961 tcgcggctga tctgtggagc agaatgggga aaactctttc gtgtaaatgc aggtaatcgc
     1021 ttacgaaacc gtgtcaaacg catcgcagta caactccccg gagcagaaaa tcctcgtcaa
     1081 catttcagag aaattccgcg ttccttttcc ttccgagtaa ataccgctaa tccaatccca
     1141 attatccgcc cagtccgact tcggatgacc aaagcaaaac agaaaaaaag cccgaataca
     1201 atacaacaca ccacaatcca ttccgactcg tttatataag ttttactgct accgaagggt
     1261 actgtcaagg attttttgcg gcatacttct cttgcttttc gtggctgact gtggaagcat
     1321 tggcaccaag tcattcgctt tctcgcagat aggcgaactc cattctgagc aggccagcnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnca ccaagtcatt cgctttctcg
     1501 cagataggcg aactccattc tgagcaggcc agcgcctgca ggcgtgtggg ctatgcccac
     1561 tccttcggac gcttgctcaa tggatgatat gcgcctttcg gactttgtgt cactaggagc
     1621 aggtttgaac ctgtgggcgt gtgggctatg ttcgctcttg gagacgcttg ctcggtagat
     1681 ggcacgcttg ttctgagccg agtgttacta caggagtaag ggagatctac tgcattttca
     1741 atgaaaggtg cggcggctgg aatcttgctc ttttggggaa tctttgcggg cgtcatgtcc
     1801 gtctacctct tgacgctctg tccctcgatc tactgggggg attctgcgga gtttgtgacg
     1861 gtggttcaca ccctcggagt ccctcaccct acgggctatc cactctactg catctttggc
     1921 aagattttcg acctgctgcc ttttgggaat cctgcctcgc gactgaatct gttttccgct
     1981 cttgctgctt ctctcggggt ggccgtggtc tttctcattg tgagagcatc cttgctttca
     2041 gcacctgaac tcagcagccg gtctttggcg atggcattct gggcggcggc ctgcctcgcc
     2101 ttcacccaac tctattggtc tcaggcaatt atcgccgaag tgtattctct cagtgcgctc
     2161 ctgattgccc ttttgatcct tgtcagtttt cggaattggc atgttcctca gcgaagctat
     2221 gagatcgctt ttggacttct ggccggtctg tgtctgacgc atcatgctca gtccgtgttt
     2281 gtcatcatgc cattgggact tctgatgata tggcgaatgg gcttctccac agggtacatg
     2341 aggcgttggc ttgctatggg agtgtgtgcc atagtgccct tgggactgta cctgtacctg
     2401 cctcttcgtt cccgtgccaa cccaccaata gactggggaa atcccgaaac actggccaat
     2461 tttctctgga tgatctccgg tggacagttc aaaatgttcc tttggaaagg tttgaccgga
     2521 tcgagttcgc tgttggcgta ctggagccgc atgactacgt tctacgcaac tcagttctgg
     2581 agccaatggc atctattgag tcttctgatt ccattcggaa ttcggaggct ctggaagcat
     2641 gaccgagcgc cctgtttcgc acttgttgtc gcttgggtgg cgttggtctt tcacgccctg
     2701 aactatcagg ttggcgactt ggaagtgtat ttcctgccca cttatttgat cgccgttgtg
     2761 ttcatggcac acgggctttt tgatatcctg gagtggcttg ggcaacgtgc gagtccttcc
     2821 tatacaaagg cgctcaatgc cgcggtgttg tgtattccac tggcctttct gacggcaaac
     2881 ctgccttacg tcagtctttc aggattccga gcagcggaag actacggagt ttcagtcttc
     2941 gagcaagtgc aggcgcccgc agtgatcatc actcattctg acaatgacat ctacccactc
     3001 tggtatcaga agtacgtctg cggcctgggg caggatgtgg ctgtcgtggg
//
LOCUS       DAOQUC010000337         3050 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_37454_length_3050_cov_3.038063, whole genome shotgun sequence.
ACCESSION   DAOQUC010000337 DAOQUC010000000
VERSION     DAOQUC010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3050
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37454_length_3050_cov_3.038063"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    932..941
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <942..1272
                     /locus_tag="PKH07_06945"
     CDS             <942..1272
                     /locus_tag="PKH07_06945"
                     /inference="COORDINATES: protein motif:HMM:NF014618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HNT34716.1"
                     /translation="PIVIGIFEAHAIQNRISNSFFPRPLTHDLLANILQEIGVTVERI
                     VVTKLEKTVVDDEEHGTFFARIHLALPSGEKVDVDARPSDAIAVAVRVECPIFAEEEV
                     LNQSGAL"
     gene            1300..1497
                     /locus_tag="PKH07_06950"
     CDS             1300..1497
                     /locus_tag="PKH07_06950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34717.1"
                     /translation="MVRFQCEGSLRAVLRQFKEQVLNKISESQLDGEVCLSIRPRNES
                     DAPEAQTGDLREQLLELLDEE"
     gene            1515..1874
                     /locus_tag="PKH07_06955"
     CDS             1515..1874
                     /locus_tag="PKH07_06955"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNT34718.1"
                     /translation="MTQVAERRSHPRVAVSLDFNIKSGEQLLAASTINLSAKGLSCML
                     SHHIPLFTKLGIFLMLPEPEDRSHEKIGPINCDGVVVRVDKVAKQGEERYSTAVFFTQ
                     IDKDAVERIQHYLQPYK"
     gene            1922..2308
                     /locus_tag="PKH07_06960"
     CDS             1922..2308
                     /locus_tag="PKH07_06960"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HNT34719.1"
                     /translation="MRCAFCWTPPVLYAALIFWASSRSEFRIADRRLMVFDKAAHFFE
                     FAILALLIMRALLHSSLKRTSAWKNVVVVFLLVSLYALTDEWHQSFVPNRHPDVMDWA
                     VDTLGAAVFLLPFLFSSVSDRLSKEG"
     gene            complement(2528..>3050)
                     /locus_tag="PKH07_06965"
     CDS             complement(2528..>3050)
                     /locus_tag="PKH07_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013181580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNT34720.1"
                     /translation="GALGFWKALKEIFPTTRHQRCWVHKTANILDKLPKKVQPRAKGM
                     LHEMYLAPTKEDALQAYSAFLRNYEAKYPRACECLEKDKEVLFTFYDFPAEHWIHLRT
                     TNPIESTFATVRLRTKRTKGCGSTQATRTMVFKLATEAQKHWRRLHGYKRIAEVLEGR
                     IFVNGVLQGQQAA"
BASE COUNT          694 a          746 c          777 g          823 t
ORIGIN      
        1 cacggtcttc acgtcgaaga ccattagttc cgaaaatggt agcgttgtcc accgtccaac
       61 ctttcttgaa aaagaaaacc gtaacctacc ctctcaagag acgccgcaga agcatctgcc
      121 agccttcttg ccctgcccca gttacacttt cttcacagtt gccgactcag agtcaagtcc
      181 gaaacactcg atttctctgc ccctcgaagt ctcaatgtga gaaagagagc atttcgaacg
      241 cgaacacgat acgaatttct tggccgagtg agtcagcaac tcaatgtcct tacttatgaa
      301 tgccttaacg acaggcattg ctctgagcgt cattcctggc tgccgactgg gcacaacttt
      361 tccgtgtcgt gttgccacag gcttccagag cggttctcgt taggttttcc ccaaacgcac
      421 ttgagttatt cacagttttt ccacggacgc tcttccccgt tcagggatgc actgacgcta
      481 acactaccga agtcactttt caagcgaaat tcaagcgaaa gtttcatgaa aattcaaact
      541 ttgctgcagt cgctgtgtac gacagtactc tcctcttgtg tcgttcttct gtgtcttcga
      601 tttcgcctaa tcagttgcac gttttttgag tgagttcaag agaaatcttt gataaaattc
      661 aggccaattt gcagatcgag aaatcgaatc atgctctgac acatctgaat tttcgcgaac
      721 gatctttgaa atcaaggttt tgcaaatatg aatgtggaga aagaaagttt tgtacttgcg
      781 atattccttt cgtttcgcct atagtgaact caggctctta ggcagaatct tttcaggacg
      841 aggttcattt catgaaagag atggagctgt tcgaactgag aatagatgac acatcccctc
      901 agcagatact gattctgcgt gaaaagcaag gnnnnnnnnn ntcccatcgt gatcggaatc
      961 ttcgaagcgc atgcgatcca aaaccggatc agcaacagtt tctttccccg tccactgaca
     1021 catgatctgc tggcgaacat cctgcaggaa atcggcgtga ccgttgagcg gatcgttgtg
     1081 accaaattgg agaagacggt tgttgatgat gaagaacacg gaaccttctt tgcacggatt
     1141 catcttgcat tgccttcagg agagaaggtg gatgttgacg cccgaccgag cgatgccata
     1201 gctgttgccg tgcgtgtcga gtgtccgatc tttgccgaag aggaggtctt gaaccaatcg
     1261 ggtgccctgt gacagggcac accaacgggt gagacgatcg tggttcgttt tcagtgtgaa
     1321 ggttcattgc gggcggtgct tcgacagttc aaggagcagg tgcttaacaa aatctctgaa
     1381 tcccaactgg atggagaggt ctgcttgagc attcgaccgc ggaatgagtc agatgcgcca
     1441 gaagcacaga caggcgatct cagagagcaa ctgttggaat tgcttgatga ggaatagatg
     1501 gaggcatgac agagatgacc caggttgcag agcgtcgttc ccatcccaga gtggccgttt
     1561 cgcttgactt caacatcaag agcggggagc agctgcttgc tgccagtacc atcaatctga
     1621 gcgctaaggg actttcctgt atgctcagtc atcacattcc actctttacg aaactaggta
     1681 tcttcttgat gcttccagag ccggaagaca ggtcacatga gaagatcggt cccatcaact
     1741 gtgatggtgt tgttgtgaga gtggacaaag tggccaagca gggcgaggag cgttacagca
     1801 cggccgtatt ctttacacag attgacaagg atgctgttga gagaattcag cattaccttc
     1861 agccctacaa gtagacgtgg gatttcgcaa ggttctccat cgttttcggg aagtcccttc
     1921 ggtgcggtgt gcgttttgct ggacgcctcc ggttctctat gctgcgttga tcttctgggc
     1981 ctcttccagg tcggagttta gaatcgcgga tcgccgactg atggtttttg acaaggcagc
     2041 ccacttcttc gaattcgcaa tcctggcatt gctcatcatg cgggcgttgc ttcactcaag
     2101 tctgaagagg acatctgctt ggaagaatgt agttgttgtg ttcctgctag tgtccttgta
     2161 tgcactcacg gatgaatggc atcagtcatt cgtgccgaac cggcatccgg atgtcatgga
     2221 ttgggctgtg gatactctgg gtgcggctgt ctttcttttg cctttcctgt tctccagtgt
     2281 atctgatcgt ctgagcaaag aggggtagtc ggaaaagtca tctctcggac aagtccacag
     2341 ctgcagtacg tgggttatga tcactcctgt tcagtgcatg ttcgcgaaag aaagagcctc
     2401 ttgcgaagtc tcgtttccct gaaggatgac acacggcgat atgtctccca gcgatgccgg
     2461 tacttcagct cacgagatat tgtcaagagt tgtgtttgga tacatgagag cgttccttct
     2521 tgagatgcta agcagcctgt tgtccctgga gcactccgtt gacgaaaatt cttccttcga
     2581 gaacttctgc gatgcgcttg taaccgtgta gccgtcgcca atgcttttgt gcctctgtcg
     2641 ccagtttgaa gaccatagtt cgagttgcct gcgtactccc gcagcctttg gttcgcttgg
     2701 ttcggagtct gactgtggca aacgttgact cgatggggtt ggtcgttctc agatggatcc
     2761 agtgttccgc agggaagtcg taaaaggtaa acagcacttc cttatccttc tccaagcatt
     2821 cacacgcccg tggatacttc gcttcatagt tgcgcaggaa tgcgctgtac gcctgcagcg
     2881 catcttcctt ggttggtgcc aagtacatct cgtgaagcat tcccttcgca cggggctgaa
     2941 ccttcttggg caacttgtcc agaatgttgg cggtcttatg cacccagcaa cgctgatgcc
     3001 gtgttgtggg aaatatctcc ttgagtgcct tccagaatcc aagtgcccca
//
LOCUS       DAOQUC010000338         3043 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_37571_length_3043_cov_3.461178, whole genome shotgun sequence.
ACCESSION   DAOQUC010000338 DAOQUC010000000
VERSION     DAOQUC010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3043
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37571_length_3043_cov_3.461178"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1821
                     /locus_tag="PKH07_06970"
     CDS             <1..1821
                     /locus_tag="PKH07_06970"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNT34721.1"
                     /translation="PRPHTLLPDFFFFSILAGASCIRRKSGMPHPESRSGYRISTAEG
                     RLSLFAAACCVLYLVCASWALISLLSRDQTPEHGDQGAYLELAVNIAEGRGFVSDNLS
                     AFQPKPDVTHRETNRQPLYPYLLSSVAGRDVGFLWRAKCLTAIIAVLLLIVVLVCVSL
                     RFGLAAGLVATVLLGIHGTFMTLATEVWCENVLVLFLVLTWFLLARSQQDGSPKRSWQ
                     WAAGAGTALGLAYLTKSSAVMVVPSLAVALLLSRPKTDLGDADGLSTERKRCIFLNRF
                     GAFLVFSLVASVAVLPHLYRTWRATGSPFYSPEISGMMWIDFGPRDAWRVYDTLPTMS
                     SYFERHTVQEASVKWFRGLRLVLRRFLELARDHPGRLPVGWLMLPLALLGLWREQRVI
                     RGMILVFALLNVASFAWYAHVDVAPRFVYPLTVFYFIYAGAGTSAAVERFFPRKIRRA
                     SVAYCVVIGALMLWSLPGLWTHANWRSKGGESKTLRAEEAETLRAIATIVQLDEVLCM
                     GPSHELAYYWVINRKRVFVPTYTELDEFRKHLDRFNVRYFLLDTSVIRRNMPLLGRHF
                     AADPFVGILQKEGIPWLEKVYENRGSPLRFILFRVREKEG"
     gene            1927..>3043
                     /locus_tag="PKH07_06975"
     CDS             1927..>3043
                     /locus_tag="PKH07_06975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34722.1"
                     /translation="MSRWSIMSVLLIAVFLLLLSLPSACEAATKTQSVLHSTSRRIAA
                     IKNAERFAWAKSARDSIVEQAEPWKQMSYDETWNLMFSSNISRSWMVWSDGFCPECHQ
                     DVRMYTWIIDIWGHPFKVQCPHCKSLFPTNDFEAFYRSGLNRSGEFDPALADRSLLFN
                     QNHPDPQDPKHLFGVDDGEGYLQEAKRWRFIGYYLSAGQWRQKIVGGVASLAEAYSIT
                     GDMEYSRRAGILLDRIADLHPSFDFATQGEVYEKRGSNGYVSVWHDACAEIQRLIYAY
                     DQIFDGIKDDLELISFLSRKATQTACPNAKSSFAEIQENIESRIFLDSIAQKHKINSN
                     YPMTPVAIMTINAVLGWPVTREAVLESLCEIARTSTVK"
BASE COUNT          643 a          796 c          810 g          794 t
ORIGIN      
        1 ccccgtccgc acacgctctt gcccgacttc tttttcttca gtattctggc tggagccagt
       61 tgcatcagga gaaagtcggg tatgccccat ccagagtccc gttccggata caggatatcc
      121 acagctgaag gccgcctcag tctcttcgcc gcggcgtgtt gtgtactcta tctggtgtgt
      181 gcgtcatggg ctcttatctc tctgctctct cgagatcaga ccccggaaca cggagaccaa
      241 ggggcatatc ttgagttggc ggtgaacatc gctgaaggac ggggcttcgt ctcggacaat
      301 ctgagcgctt tccagcccaa gccagatgtg acccataggg aaacaaatcg tcagccgctc
      361 tatccctatt tgctctccag cgttgccggt cgggatgtgg gttttctgtg gcgtgcgaaa
      421 tgccttaccg ccatcattgc tgtcctgctt ctcattgtgg tgctggtctg cgtctccctg
      481 cgtttcggtc tggcagccgg tcttgttgcc actgtgcttt tgggcattca tggcacattc
      541 atgactcttg ccacagaggt ctggtgtgag aatgtacttg tcctttttct tgtcttgacg
      601 tggtttctgc ttgctcgatc acaacaggat ggatccccaa agagatcatg gcaatgggcg
      661 gcgggcgcgg gcacagcgct gggcttggca tatctgacaa aatccagtgc tgtcatggtg
      721 gtgccgtctc ttgcagtggc actgctgttg agcagaccga aaacagatct cggagatgca
      781 gatggcctct cgactgaacg gaaacgatgc atcttcctaa ataggttcgg tgcctttctt
      841 gttttcagtc ttgtcgcatc agttgcggtc ttaccccatc tctacagaac ttggcgcgct
      901 acaggcagtc cattctacag ccctgagatc tcaggcatga tgtggataga tttcggtccg
      961 cgagacgcct ggcgtgtcta cgacacattg cccacgatgt cgagttactt tgaacgccat
     1021 accgttcaag aagcctctgt gaagtggttt cggggcttgc gtcttgttct tcggcgcttt
     1081 ctcgaactgg caagggatca tccgggcagg ctgcctgtcg gctggctgat gttgcctctt
     1141 gcgctgctcg gtctgtggcg tgagcaaagg gtgattcgag ggatgatttt ggtcttcgct
     1201 ctgctgaacg tcgcgtcctt cgcttggtat gcacacgttg atgttgcccc tcgatttgta
     1261 taccccctga ccgtattcta tttcatctat gcaggcgctg gaacgtctgc agcagttgaa
     1321 aggttctttc cccggaagat tcgacgtgct tctgttgcct actgcgttgt tattggagcg
     1381 ctgatgctct ggagtctccc gggtttatgg actcacgcaa actggcgttc caagggggga
     1441 gagtcaaaaa cacttcgagc tgaggaagcc gaaactctca gagctatcgc aaccatcgtc
     1501 cagctggacg aagtcctctg catgggaccc agccacgaac tggcttacta ctgggtcatc
     1561 aatcgcaaga gagtttttgt tccgacgtat actgagctcg atgagttccg aaagcatctt
     1621 gaccgattca atgtgagata ctttctgctc gacacatctg tcatacggcg aaatatgccc
     1681 cttctgggaa ggcacttcgc tgcagaccct tttgtcggca ttcttcagaa ggaaggcatt
     1741 ccctggcttg agaaggttta tgagaaccgc ggatccccgc ttcgtttcat tctgtttcga
     1801 gtgagggaga aggaagggta gatatggatt ccgcactcac tttgttgcgc aggcctggtc
     1861 tcgagacgat ttcgaacaac agcctcgcat agttcggata gcagactttc acgaaggagt
     1921 atccaaatgt cacggtggag catcatgagc gttttgttga tcgctgtctt tctcctcttg
     1981 ctgtcgcttc cctctgcgtg tgaggcggca acaaagacac agagcgttct tcactcgacg
     2041 tcacgacgca tcgccgcgat caagaacgcc gaacgtttcg catgggcaaa gtcggcacga
     2101 gattctatcg tcgagcaggc ggagccttgg aagcagatgt cctatgatga gacctggaat
     2161 ctgatgttca gttccaacat ctctcgaagc tggatggtct ggtctgacgg tttctgtcct
     2221 gaatgccatc aagacgttcg aatgtacact tggatcatag atatctgggg tcatccattc
     2281 aaggtgcagt gcccacactg caagagcctc ttcccaacca atgacttcga agcgttttac
     2341 agatccggtc tgaaccgatc aggagaattt gatccagcac tggcagatcg ctcactcttg
     2401 ttcaatcaaa accatcccga cccacaagac cccaaacacc tcttcggtgt ggatgacgga
     2461 gaaggctatc tgcaagaggc caagcgatgg cgattcatcg gttactatct cagtgccggt
     2521 caatggcggc agaagatcgt cggcggtgtt gcgagtcttg cagaagctta cagcatcaca
     2581 ggggacatgg aatactcacg gagagcgggg atacttcttg atcgaattgc tgacctccat
     2641 ccgtcctttg actttgcaac gcagggcgaa gtctacgaaa aacgcggctc caacggctat
     2701 gtcagcgttt ggcatgatgc ctgtgcagaa attcagcgac tcatctatgc ctatgaccag
     2761 attttcgatg gtatcaagga cgatctcgag ttgatttctt tcctttctcg gaaagcgaca
     2821 cagacggcat gtcccaatgc caagagttcg tttgcagaga ttcaggaaaa catcgagtcc
     2881 aggatatttc tggattcaat cgcgcaaaaa cacaagatca actcaaacta ccccatgacc
     2941 cctgtcgcaa tcatgacgat caacgcggtt cttggatggc ctgtgactcg tgaggcggtg
     3001 cttgagtcac tgtgtgagat cgctcgtacc tcgacagtga agg
//
LOCUS       DAOQUC010000339         3037 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_37667_length_3037_cov_3.557679, whole genome shotgun sequence.
ACCESSION   DAOQUC010000339 DAOQUC010000000
VERSION     DAOQUC010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3037
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37667_length_3037_cov_3.557679"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..955
                     /locus_tag="PKH07_06980"
     CDS             <1..955
                     /locus_tag="PKH07_06980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34723.1"
                     /translation="YREVTVPEPGPYEIVVRTHYSWISNGTEGSLLRGERVNGETPYK
                     PGDRWPFPIVAGYQSTGIVESVGDSVQLVQPGEWVFCPMGRIDGMYEAWGGHISLKVA
                     HETQVWKIPEDVSPIAASGLVLTQVGYNCGIRAPINISDVALVIGDGMVGHWAAQTLH
                     WRGAKVILAGKHDDRLSFFPEGNNRYRVNVTRQDILEVVRAIAPCGIQVLVHSSGPLS
                     SVMECHPLMKHDGHIVSAGFIKEDSLIDVQRLRFGELALHCPSGWSRQRMDETLSLIA
                     SGYLDTESLITHRFPVKDAAEAWNVIIARTETVLGVILEWV"
     assembly_gap    1157..1166
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1167..2040
                     /locus_tag="PKH07_06985"
     CDS             <1167..2040
                     /locus_tag="PKH07_06985"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HNT34724.1"
                     /translation="VTTCPHWDMHILGGVPMLAGWPLEYPYTLGQPGHEACGDIEAVG
                     SEVTEVSVGQRVCAWRDQGHHRPGCYAQYAILDVENVIAVPRAIPPEHCASLELAMCI
                     SAHILYAEKLDAIAGQRIGVFGLGPAGLVCIQLLRAAGASEVIGFDPMPERREMALSF
                     GAARAIDPSGGEAAAFPRRGQSGALHATFDCVGVPKAVHQAMELTNTLVILFAVQREP
                     YIFAPQYWVGMALVGAQMHSRAAAEFAAAKLASGQLNLAPLVTRTMRLEDYGEAVGLL
                     KNREAIKVAFMPQL"
     gene            2275..>3037
                     /locus_tag="PKH07_06990"
     CDS             2275..>3037
                     /locus_tag="PKH07_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012685284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNT34725.1"
                     /translation="MSTPVAAEKEALLEVRELQTHFLLREGIARAVDGVSFSVSRGET
                     LSLVGESGCGKSVTALSILGLVPSPPGRTVGGEILFDGEDLIHKSDREMRAVRGNRIS
                     MIFQEPSSALNPVFTVGYQLMEVFRLHKRLSVREARAQSILMLEKVRMASAADCMNRY
                     PHELSGGMCQRVMIAMALACQPELLIADEPTTALDVTVQARILELMQDLKQQFGSSVL
                     LITHDLGVVAEVAEKVVVMYAGEVVEMAPVQSLFAN"
BASE COUNT          617 a          759 c          947 g          704 t
ORIGIN      
        1 ataccgcgaa gtcactgttc cggagccagg tccctatgag atcgtcgttc ggacgcacta
       61 ttcatggatc agcaatggga cagaaggctc cctgctgcga ggagaacgcg tcaacggcga
      121 gactccctac aaaccaggag atcgatggcc atttccgata gtggctggat accagagcac
      181 gggcatcgtc gagagcgttg gggactccgt gcaattggtt cagccgggcg agtgggtctt
      241 ctgccccatg ggacgcatag atggcatgta cgaagcttgg ggagggcaca tctctttgaa
      301 ggtggctcac gaaactcaag tctggaagat accggaggac gtttcaccca tagcagccag
      361 tggattggtt ctgacgcagg ttggctacaa ctgtggcatt cgagcgccca tcaacatcag
      421 cgatgtcgct ttagttattg gcgatggcat ggttggccat tgggcagcgc agacactgca
      481 ctggcgcggc gcaaaggtca ttcttgccgg caagcatgac gatcgacttt ctttctttcc
      541 agaaggcaac aaccgctatc gagtgaatgt caccaggcaa gacatcctcg aggttgttcg
      601 agcgatagcc ccatgcggaa ttcaggtgct tgttcatagc agcggaccgt tgtcgagtgt
      661 gatggagtgt catccgctca tgaagcacga tggacatatc gtttccgcag gcttcatcaa
      721 ggaagactct ttgatcgatg tccagagact ccgcttcggc gaactggcgc tgcattgtcc
      781 ttccggatgg tctcgacagc gaatggacga gacgttatct ctgatcgcga gtggatatct
      841 ggatacggag agtctgatca cgcaccgatt tccggtcaaa gacgcggcgg aggcgtggaa
      901 cgtgatcatc gcccgaacgg agacagtgtt gggtgtaatt cttgaatggg tctgaacgcg
      961 ccctcacgtg ggctgtgtcc acttccctgg acgcttgctc ggcaaaggat gcgcctgttc
     1021 tgaaccgagt ttcagtagga gaaaggaagg gaattgatga aagcactgca gataatggga
     1081 cctgagaagt atgtgatcct ggatgttcct atgcccaagc ctgggcctcg agaagtcctg
     1141 ctgaaggttc tggcggnnnn nnnnnnggtg accacttgtc cgcattggga tatgcacatc
     1201 ctgggtggtg tgccaatgct tgccggctgg ccattggagt atccctacac gttgggtcaa
     1261 ccgggtcacg aagcctgcgg tgacattgag gcggtcggca gcgaggtgac cgaggtgagc
     1321 gttggtcagc gtgtctgtgc ttggcgcgat cagggtcacc atcgtccggg ctgctacgcg
     1381 caatacgcca tcctggatgt ggagaacgtg attgcggttc cgcgagcgat tccgcccgag
     1441 cattgcgcgt ctttggaact ggccatgtgc atcagcgcgc acattctgta cgcggagaaa
     1501 ctcgacgcca tcgcgggaca aaggatcgga gtctttggtc tcggccccgc gggcttggta
     1561 tgcattcagc ttctccgtgc ggcaggtgcg tctgaagtca tcggtttcga tccgatgccg
     1621 gaacgccgag agatggcctt atccttcggg gcagcccgag cgatcgatcc ttcaggagga
     1681 gaggccgccg cttttccccg cagaggacag tcgggcgctt tgcatgcgac cttcgactgt
     1741 gtgggtgttc cgaaagcggt tcatcaggcg atggaattga ccaacacact ggtgatcctt
     1801 tttgctgttc agcgcgaacc gtacatcttc gcgcctcagt attgggttgg gatggcgctg
     1861 gtcggcgcgc agatgcattc gcgtgccgct gcggaattcg ccgcggcaaa gttggcttca
     1921 ggtcagctga acctggcgcc tctggtcact cgaaccatgc ggctggagga ttacggtgag
     1981 gcggtcggac tgttgaagaa tcgcgaagcg atcaaggtcg cgttcatgcc ccagttgtag
     2041 gaggacatcg gacggcgggc atgtatctac atggagcagt acggactgcg aggaggtctc
     2101 caacctgcag atgcactcat cacagcgact gcaattgaaa acgcggacac gctttgctct
     2161 ggcaacgcca gacactatgg tatcatttcc gatatccttc tgaaggtctt tcgcgcctga
     2221 ggttcggcat cggctttaca agtagtcctc ccttcggcta ggatgccttc acttatgagc
     2281 acacctgtgg cagctgaaaa agaggcactt ctggaagtca gggaactgca gacgcatttt
     2341 cttttgcgag aaggcattgc gcgtgctgtg gacggggttt cattctctgt atctcgtgga
     2401 gagacgctga gtcttgttgg ggaatccggc tgtggcaaga gtgtgaccgc tctctcgatt
     2461 ctgggtctgg tgccgtcccc gccaggaagg acggttggtg gcgagattct ttttgacggt
     2521 gaagacctga ttcacaaatc ggatcgggag atgagagcgg ttcgaggcaa tcgcatctcg
     2581 atgatctttc aggagccttc cagtgcgctc aatcccgttt ttaccgttgg gtatcagttg
     2641 atggaagtct tccggctcca caaaagactt tcggtacgtg aggcgagggc gcaatccatc
     2701 ctgatgctgg aaaaggttcg catggcgagt gcagcggact gtatgaaccg gtatccgcat
     2761 gagttgtctg gtggcatgtg tcaaagggtc atgatcgcga tggcgctggc ctgtcagcca
     2821 gagttgctta ttgctgatga gccgacgacg gcacttgacg tgaccgttca ggccaggatt
     2881 ctcgaactca tgcaggatct gaagcagcaa tttggatcgt ctgtacttct gatcacgcat
     2941 gatctggggg ttgtcgctga ggtagcggag aaggttgtcg tcatgtacgc cggtgaagtg
     3001 gtggagatgg cacctgtgca gtcgctcttt gccaacc
//
LOCUS       DAOQUC010000340         3033 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_37744_length_3033_cov_2.925453, whole genome shotgun sequence.
ACCESSION   DAOQUC010000340 DAOQUC010000000
VERSION     DAOQUC010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3033
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37744_length_3033_cov_2.925453"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1439)
                     /locus_tag="PKH07_06995"
     CDS             complement(<1..1439)
                     /locus_tag="PKH07_06995"
                     /inference="COORDINATES: protein motif:HMM:NF039640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6057 family protein"
                     /protein_id="HNT34726.1"
                     /translation="MTESATVFRYCRTGCIRTVLLASFVLVFLWKAVRPELVYHNQSP
                     VFSFSSQFLSEYMGTPGEPVRYLSHLVYLSYLWPALGALLTTLLILGICLLAGRYFRV
                     SGISHFSSMHLIPGAFLVLLHNSYQHSLSLDIGFAVALVAALTYARHGAWHLFARVVL
                     FLVTAGVIFYLATGAYLVFVALSAVFECVRNRRAWLASVLLVLSLFIPLAYSLLVFGV
                     SAGKAYASLIPPEYDGKIPSALVCLWLSCPGLVIGVLAWEKILARKMDWEARERMRRG
                     DIAEESHSVQEKSHRHRGGQREKSIIRRSLLASSVLAVVLYFSFPMHVRTLIEIDYHA
                     TNEEWEKVLVASKRLPVNLMTHFAFHDTNRALFHLGVYPERMFEFPQTDQLNTLMPPE
                     KLSESMHACLKFSDLFYDLGLISESEHLAYEALSSYGNHPAILKRLVQTNALKGRAQA
                     ARQFLNVLQTYPAARKWAKKFRTFLDSEP"
     gene            1829..2665
                     /locus_tag="PKH07_07000"
     CDS             1829..2665
                     /locus_tag="PKH07_07000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34727.1"
                     /translation="MKKNTCLMSFICVLVLALSWSCVALAVQQETPFIYTTGFEPDEV
                     PAFSVGTLPQNGWESSGTATITDSIAYSGVQSLAISPQATVDKAMDVAGHQEVWLEGW
                     YKTTPEPEYPDLAVLEPGSCLVLFHSIEGISCLDGDGQGNGAWISTGVMVDPARWYRV
                     TIRQNYQTHKWDCFVDGYLKLRGLGFKNNINKLSGFRLQQGENNNGNLDEFVVQTEPL
                     IPSFGYQELFRFSRTWHWLNETPYEAPFNESEFNRYNRMRDELINGSDLTELIKAWHW
                     DR"
     gene            2832..>3033
                     /locus_tag="PKH07_07005"
     CDS             2832..>3033
                     /locus_tag="PKH07_07005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34728.1"
                     /translation="MSKQELFGARNQRLWLLFGISVVLSGLCCVPWLAFCQTIIVDDL
                     HSAFEATEGWQQGDVLVRRHSTT"
BASE COUNT          801 a          736 c          768 g          728 t
ORIGIN      
        1 aatggctcac tgtccaggaa agttcggaac ttctttgccc acttgcgagc tgccggatat
       61 gtctgaagaa cattgagaaa ctgtcgtgcg gcttgcgccc tgcctttcag ggcattggtc
      121 tgaacgagtc tcttcaaaat agctgggtga ttaccatagc tggacaatgc ttcatatgcg
      181 agatgctcgg attcgctgat cagccccaga tcataaaaga gatcactgaa tttcaaacag
      241 gcatgcatag actcactcag cttttcgggg ggcatgagag tgttcaactg gtcggtctgt
      301 ggaaactcga acatacgctc cggatagaca ccaagatgaa acagcgctcg gttggtatca
      361 tgaaaggcaa aatgagtcat aaggtttacc ggcagccgct tggatgccac gagaactttt
      421 tcccattcct cattggtggc atgatagtct atctcgatca gagtcctgac gtgcatggga
      481 aatgaaaaat acagcaccac cgcgagaact gaactggcga gaagagagcg gcgaatgatg
      541 ctcttctctc gctgacctcc tctgtgccga tgagacttct cttgcacaga atgcgactct
      601 tctgcaatgt caccacgacg catccgttct ctggcttccc aatccatttt cctagcaagg
      661 atcttttccc aagccaaaac accgatgact agacctggac aagacaacca aagacacacc
      721 agtgcagagg gaattttccc atcgtattca ggagggatta gactcgcgta ggctttccca
      781 gcactcacac caaaaacaag caatgaatac gccagaggga tgaaaagtga gagaaccaac
      841 aacactgagg caagccaggc acggcgattt ctaacgcatt cgaatactgc tgacaatgct
      901 acgaaaacga gatatgcacc cgttgccagg tagaagatca caccagccgt tacaagaaac
      961 agaaccacac gggcaaacaa gtgccaagcg ccatgtcggg cgtatgttaa agccgcaaca
     1021 agtgccactg caaatccgat gtccagactc agcgaatgct gatagctgtt gtgcagaaga
     1081 accagaaatg ctccagggat cagatgcatg gaagagaaat gggagatccc actgacacgg
     1141 aaataccttc ctgccaagag acagatcccc aatatgagca gtgttgtcag aagggctccc
     1201 agtgctggcc acaggtacga aaggtacacc agatgggata ggtaccggac tggttctcct
     1261 ggtgttccca tgtactccga gagaaactgg gaactgaagg aaaagacagg cgattggttg
     1321 tggtagacca gctccggtcg gactgccttc caaagaaaga ctaacacgaa ggacgcaagc
     1381 aggacagtac gaatacagcc agtccggcaa tatcgaaaga cggtggcaga ttcagtcaat
     1441 gcttgctctc tcctcgtaag attcgcaaga gtagatatga gatctgtttc tgcatcttgc
     1501 catcatactc caacatgatg tcaagactca caattgctgt catattctcc cgattgaagc
     1561 aaacagttca gttcaaactc attcagccac tcatatcgtg gcataacaat ttaggatgct
     1621 accctaactg gcgttctgaa gcaagtcgca cggacgacct ggaaatgaga tgggatccag
     1681 gctggatatc ctctttcgat acagcacccg ttttctgaaa gcggagacgg aattggttga
     1741 caaaggacgc aatatttcct tattatcccc aaagtgaaca tgttctggcg gaatacgcca
     1801 atctcgtttc ttagggagtc tgacagaagt gaagaaaaac acctgcctga tgtcatttat
     1861 ctgtgtcctt gttttggctc tttcctggtc gtgtgtggct cttgccgttc agcaggagac
     1921 tccatttatt tatacaacgg ggtttgagcc tgacgaagtt cctgcctttt ccgtcggaac
     1981 tcttcctcag aatggatggg agtcatctgg aaccgccacc atcaccgatt ccattgccta
     2041 cagcggggtg cagtctctgg ccatttcccc acaggccact gtggacaagg cgatggacgt
     2101 tgccggacat caggaagtct ggcttgaagg atggtacaaa accacaccag aacccgagta
     2161 tcctgatctt gccgttctgg aacctggcag ctgccttgtg ctgttccatt cgatcgaagg
     2221 aatctcgtgt ctagacggtg acggacaagg caacggggcg tggatctcta ccggagtcat
     2281 ggttgaccct gctcgttggt accgagtgac gattcgccag aattaccaga cacacaagtg
     2341 ggactgtttt gttgatggct atctgaagtt gcgtggtctt ggtttcaaga acaacatcaa
     2401 taaactttcc ggtttcagac tacagcaagg tgagaacaac aacggaaatc tggacgaatt
     2461 tgttgttcag acagagcctt tgattccatc gttcggctat caggagctgt tccgtttctc
     2521 tcgaacctgg cattggctga atgagacacc ctatgaggct cccttcaatg aaagcgagtt
     2581 taaccgctac aaccgaatgc gtgacgagct gatcaacggt tcggacttga ctgagttgat
     2641 taaggcttgg cattgggaca ggtgatacga caggaatttc ctatgcagcg attttcgggg
     2701 gcgaggcgac tgccaacttc gtccccgttt cttttgtccc catggcataa gtaggtaact
     2761 acttaggctt gcttttgcgg atgtgaacag ttataaatgg gctgagtatt tcgcgaggtg
     2821 aggagtctaa catgtccaag caagaactct ttggcgcaag gaatcagaga ctgtggttgc
     2881 tctttgggat ttctgtggtg ctcagcgggc tttgctgcgt gccgtggttg gccttttgcc
     2941 aaaccatcat tgttgatgac cttcattctg cctttgaggc aacagaagga tggcagcagg
     3001 gtgatgttct cgtcagaagg cattcaacaa cct
//
LOCUS       DAOQUC010000341         3032 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_37757_length_3032_cov_3.668794, whole genome shotgun sequence.
ACCESSION   DAOQUC010000341 DAOQUC010000000
VERSION     DAOQUC010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3032
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37757_length_3032_cov_3.668794"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            60..1640
                     /locus_tag="PKH07_07010"
     CDS             60..1640
                     /locus_tag="PKH07_07010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34729.1"
                     /translation="MATNTSHSHIVSVGQVFTPITWAEWLLLRWGVFDAWITGASVCD
                     PTAGEGVFALALFRLARARGIAVKPEMLVKLTLIEVQPTRLEAFRVKASGDFGIEFPN
                     SNLFAQDIITEPHPCKYDILVGNPPWANFTDLPPSYKEQLKPFFVAEGLVPDKKRVLL
                     GSSRTDIAALVLKVVLGRLLGEEGFGCFYLPSSLFFGDDAHIGFRDYTANHRFFSVDE
                     VCEFTLSRVFDGVGTSYCCARFQMDVQQSFPVRYFRESSGDWIEHSAMPLKRPDDQWR
                     VLEHGDNSCFDGPLGIELDATQKPRQGVNTCGANSVFIFDRKPSHLPSEYLYPLATKE
                     VWREGNGEPHKWILLPYDAKTGRPLPWSKIEEITELRDYLNGNRGLLQSRKGTLIQSA
                     ISKGLWWSLLGVGPYSFAPYKVIWEAYGQKQFRPIRLHEFSGHVWQANQAMHAFIPCW
                     SEKDAKRIQEGLLNPDILSLLRQLNGDGKCNWAQPGKIQKILAYETPQSHQPLLCAER
                     REECRTRGSSVRRVPRRR"
     gene            2217..>3032
                     /locus_tag="PKH07_07015"
     CDS             2217..>3032
                     /locus_tag="PKH07_07015"
                     /inference="COORDINATES: protein motif:HMM:NF033550.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HNT34730.1"
                     /translation="MQLPMSTSNSISVLGEWEGYRVGRTQRVEGVKGKGCEEVWIELL
                     PEETGSMVCSACGGKAKSIHEVSERMIRDLPILGAKTWLFVKRRRVLCERCGPKLERL
                     SWLDRHQRVTKRFAESIVRLCEVLAISQVALYFDLGWETVKNIHKRALKRRLEPVELS
                     GLEVIAMDEFAIQKGQQYATCIVNPKNKQVLWVGRGRGREDIRPFFQLLGPERCKALK
                     AVAMDMNGAYEQEVRSHCPNAQIVYDLFHVLAKYGREVVDKVRAEEGRRLQQAD"
BASE COUNT          731 a          723 c          891 g          687 t
ORIGIN      
        1 ccagcgggtc agtcgcgacg ccctgaaacg tatcgcagca ctggaagagt tcaggaagaa
       61 tggctacgaa cacttcacat tcgcacatcg ttagtgtcgg ccaagtgttc acgcccataa
      121 cttgggctga atggcttctt ctgcgttggg gtgtctttga cgcatggatc acgggcgctt
      181 ccgtctgtga cccaactgcg ggcgagggcg tctttgcact cgccctcttc aggcttgcta
      241 gggccagggg aattgcagtg aagccagaga tgctcgtgaa gttgactctc attgaggttc
      301 aacccacgcg cctagaggca tttcgggtca aggcatctgg cgattttggc atagaatttc
      361 cgaattcaaa ccttttcgcg caagacatca ttacagaacc ccatccctgc aagtacgaca
      421 tcctcgtagg caatcctccg tgggccaact tcactgacct gccgccatcc tacaaagagc
      481 aactgaagcc cttcttcgtg gcggagggac tggtgcctga caagaaacgg gttctactcg
      541 gttcgtcacg gacggatatt gccgctctcg tgcttaaggt cgtgttgggg cggcttcttg
      601 gtgaagaagg ctttggttgt ttctatctgc catcttctct gttttttggt gacgatgcgc
      661 acatcggatt ccgggattac acagccaacc acagattctt ttcggttgat gaagtctgcg
      721 aattcacttt gtcaagagtt ttcgacggtg tcggcacgtc ttattgctgc gcacgttttc
      781 aaatggatgt ccaacagagc ttccccgtcc gctatttcag agagtcctcg ggtgactgga
      841 tcgagcattc cgccatgccc cttaagaggc ccgatgatca atggcgtgtc ttggagcatg
      901 gtgacaattc ctgttttgat ggtccgttgg ggatagaact ggatgcgacg cagaagccac
      961 gccaaggcgt caacacttgc ggtgcaaata gcgtgttcat ctttgatagg aaaccgagtc
     1021 atctgccgag tgaatatctt tacccgctgg caacaaagga agtatggaga gagggcaacg
     1081 gcgaaccaca taagtggatc ctgcttcctt atgatgccaa gacaggcagg ccactcccat
     1141 ggtctaaaat tgaagaaatc actgaactca gagactactt gaacggcaat cgcgggttgc
     1201 ttcagtcccg gaagggcaca ctcattcagt cggcgatttc caaaggtctc tggtggtctt
     1261 tactcggagt cggtccatac tcatttgccc cctataaggt catttgggag gcctacggcc
     1321 agaagcagtt caggccaatt cggcttcacg agttctcagg gcatgtgtgg caggctaatc
     1381 aagcgatgca cgcatttata ccttgctgga gcgagaaaga tgctaagcgg attcaagagg
     1441 gattgctaaa cccagatatt ctatcactgc tgagacaact gaatggcgat gggaaatgca
     1501 actgggctca accaggaaag atccagaaga tacttgccta tgagacccct cagagccacc
     1561 agcccctttt gtgcgcggaa cggagagaag agtgtcgaac caggggttcc agcgtacgtc
     1621 gcgttccgcg ccgccgctga cccctggcgt tcaatgtggg tgaacgatgt cgaaggataa
     1681 acacaagaag caccggtctc attccaagag cgagtaggcc gggttccccg gaggctgtct
     1741 caaaactcat cagagcgaca ccttctgtca ttctgaacgg agcaaagcaa agtgaagaat
     1801 ctcgattcta tggggctttc agcgatcaga gagatgcttc gcttcgctca gcatgacaac
     1861 cccaagaggg ttttgagaca gcctcccccg aaaccgggat cttggctaca gactgagcga
     1921 agaagtcgta ggggcgctgt ttgctgcgct ctttctggag ggcaagccct actcctacaa
     1981 accgaacgcg gttgctatga gtgggtagca acgctgccga cgatgttctt gtggcttcgg
     2041 cagcgcgcaa aggcttgcta ctaaagctcc tcattggcct acggcgcacc tgaaggggat
     2101 gacaaatccc cccttgatcc ccccttatcg aaggttcttc aaggaaaagc ggggtagagg
     2161 gaggacaaag gcagcgttaa tatccttcaa aagcatgtgt gttaaaggag aaggatatgc
     2221 agctgccaat gtccacgagc aatagtatca gcgttttggg agaatgggaa ggctatcgtg
     2281 tagggagaac gcaacgtgta gaaggcgtga agggaaaagg gtgtgaggag gtgtggatcg
     2341 aactattgcc ggaagaaaca gggagtatgg tctgcagtgc ctgcggtggg aaagccaagt
     2401 cgattcatga agtgagtgag cgtatgattc gagacctgcc gatattggga gcgaagacat
     2461 ggttgttcgt gaaacgcaga cgggtgttat gcgagaggtg tggaccgaaa ctggagcgac
     2521 tgagttggct ggaccggcat caacgggtaa cgaaacggtt cgcggaaagt attgtaaggc
     2581 tgtgcgaggt actggctatc agccaggtgg cgctctattt tgacttgggt tgggagacgg
     2641 tgaagaacat ccacaagcgg gccttgaaga ggcggttaga gccggtggaa ctctctgggt
     2701 tggaagtgat agcaatggac gagttcgcca ttcagaaagg gcaacaatac gcgacatgca
     2761 ttgtcaatcc aaagaacaag caggtgcttt gggttggtcg aggccggggg cgtgaagata
     2821 ttcgtccctt ctttcaactg ctgggtcccg agaggtgtaa ggcgcttaag gctgtggcga
     2881 tggacatgaa cggcgcttat gagcaagagg tgaggagtca ttgtcccaac gcgcagatcg
     2941 tctatgacct gtttcatgtg cttgccaagt atggtcggga agtcgtggac aaggtccggg
     3001 ccgaagaagg ccgacgactc caacaagcag at
//
LOCUS       DAOQUC010000342         3029 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_37823_length_3029_cov_2.474109, whole genome shotgun sequence.
ACCESSION   DAOQUC010000342 DAOQUC010000000
VERSION     DAOQUC010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3029
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37823_length_3029_cov_2.474109"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1000)
                     /locus_tag="PKH07_07020"
     CDS             complement(<1..1000)
                     /locus_tag="PKH07_07020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34731.1"
                     /translation="MRQTTIVILFLFTHLGAYSAMGETQSNQSSWDLYSDTWVATDGL
                     GRALPTHSEVGAPRKDRFVGVFYFLWLGQHSKTGPHDVTKILQAHPEAMQDAGHPAWG
                     PEHAYHHWGEPLFGNYVSDDAYVIRKHAEMLSDADVDVIFFDVTNNATYRNCYMTLCR
                     VFSEVRRDGGRTPQIGFLCPFGNPAKVVQELYTDLYAPNLYPDLWFCWEGKPLILADP
                     AHVEDEHRNFFSFRKPMPSYFAGPSGPNQWGWLEVYPQHVFRNALGEAEQMTVGVAQN
                     AVEGRLGSMSESKGACGRSYHSGHKSEDPDAVFWGLNFEEQAKRALEVDPKFIFITG"
     gene            complement(997..2664)
                     /gene="ccsA"
                     /locus_tag="PKH07_07025"
     CDS             complement(997..2664)
                     /gene="ccsA"
                     /locus_tag="PKH07_07025"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HNT34732.1"
                     /translation="MKILAPLLAVSLFILLVSVDIIRGETSVLAPLHDIAIQDRGRLK
                     PLDTFARETLRTITGNEVWASFKGSGFPMSGQPVSLLLSMMLKWQTWSEEPVIKVRLA
                     ALRSDLDFPSRRVSFKMLSEHKVFREALRRAFTSKEQGKELTEKERALLELFQQVELF
                     RSLSKPQDALAIICPTTGEHREEMTWLTVNQAIEAGFDNADVIAEAFTALETAVAESQ
                     FEVLLESANRLADLLAAAKSHLRHPQLELHYNRLKPFRLASILLLVSAFFWLVALLVE
                     KRTALVAAWALSVLGIASQIWGFTLRVLISGRAPVTNMYESLVFMALGIVLVSALFEG
                     LSRSKMLMLSGSLTAGMLLVMAQVLPIDSSIAVIVPVLRSNFWLVLHVLVIMLSYSAM
                     AEAWALGHVILLRSTLKPEGLDALKSEVTFLYRTLQVGVLLLTAGIVLGAMWANVSWG
                     RYWGWDPKETWSLICLFGYLALLHARSVNWVGRFGLAVGSILAFMLVVMCYYGVNFLL
                     RQGLHNYGSGSGGVPYAIAYLVADAVFVLAVFWRHRRITQIGSLENQ"
     gene            complement(2661..>3029)
                     /locus_tag="PKH07_07030"
     CDS             complement(2661..>3029)
                     /locus_tag="PKH07_07030"
                     /inference="COORDINATES: protein motif:HMM:NF016995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HNT34733.1"
                     /translation="LRLSQREVPLGFQIRLLEFGNPTYGGTGMAARYYSTVDVSPTDK
                     PDLVEKHTISMNQPLVMNGFRLFQSSYYQDPDGTSVSVLSVSRDPGVPLVYSGSCLLI
                     VGIALTFYLPRQRTGAAQNG"
BASE COUNT          788 a          834 c          774 g          633 t
ORIGIN      
        1 agccggtgat gaagatgaat ttcggatcga cctccagtgc gcgtttcgct tgctcttcga
       61 aattcagtcc ccagaaaacc gcatctggat cttctgactt gtgcccactg tgataactcc
      121 gcccgcatgc gcctttgctc tcgctcatgg agcccagtct gccttccacg gcgttctgtg
      181 ccacaccgac ggtcatctgt tccgcttctc ccaatgcatt gcgaaacacg tgttgtggat
      241 agacttcaag ccagccccac tgattcggac cgctgggccc tgcaaagtaa gacggcatcg
      301 gtttgcgaaa gctgaagaaa ttccggtgct cgtcttccac gtgtgctggg tccgccagga
      361 tgagaggctt gccttcccag cagaaccaca gatcaggata cagattcggg gcgtagagat
      421 cggtgtagag ttcctgaacc accttggcgg gatttccaaa cggacacaag aaccctatct
      481 gcggtgtcct tcctccgtct cggcgaactt ctgagaacac tctgcacagg gtcatataac
      541 agttgcggta ggtggcattg ttggtcacgt caaagaatat gacatccaca tccgcgtccg
      601 agagcatctc cgcgtgtttg cgaattacat aagcatcgtc ggaaacataa tttccaaaga
      661 gcggttctcc ccagtgatgg taggcatgct ccggtcccca tgcgggatgg ccagcatcct
      721 gcatcgcttc ggggtgcgcc tggaggatct tggtgacatc atgcggaccc gtcttgctgt
      781 gctgacccaa ccagagaaag tagaacactc caacgaagcg gtcttttcgt ggggcaccaa
      841 cttcactgtg tgtcggcaac gcacgaccca aaccatcggt ggcaacccag gtgtcgctgt
      901 aaagatccca agaagattga ttcgattgtg tttctcccat ggctgaatag gctcctagat
      961 gagtgaacag gaataggatc acgatagttg tctgtctcat tggttctcaa gactccctat
     1021 ctgagtgatt ctccggtgcc gccaaaacac agccagaaca aagactgcat cggcaacaag
     1081 gtaagcaata gcatacggaa caccaccgga accagaaccg tagttgtgaa gaccctgcct
     1141 cagcaggaag ttcacgccgt aataacacat gaccacaagc atgaatgcca ggatgctccc
     1201 aaccgccaag ccaaaccgtc ccacccagtt gacagaacga gcatgcaaca aggcaaggta
     1261 tccgaagaga cagatcagcg accacgtctc tttgggatcc catccccagt atcttcccca
     1321 tgagacgttt gcccacatgg caccaagaac aatgccggca gtcagcaaga gcactccaac
     1381 ctgaagtgtg cgatagagga atgtgacctc agacttgagc gcgtccagac cctccggctt
     1441 caaggtcgag cgcagcagaa tgacatgccc cagcgcccaa gcctctgcca tggcgctgta
     1501 actgagcatg ataaccagta catgcaagac cagccagaag ttgcttcgaa gtactggcac
     1561 aatgacggcg atgctcgaat cgatcggtag cacctgcgcc atgaccaaga gcattcctgc
     1621 ggtcaatgaa ccagacagca tcagcatctt ggaacgcgaa agtccctcaa aaagggctga
     1681 caccagcacg atcccaagag ccatgaaaac caaagactcg tacatgtttg tgaccggcgc
     1741 acgaccactg atcaagacac gcaatgtaaa tccccaaatc tgagaggcga tccccaaaac
     1801 tgaaagcgcc caggcagcaa caagagcagt tcttttctcg acaagtaagg caacaagcca
     1861 gaagaacgcg gacacaagca agagaatgga tgccaaccga aaaggtttga gacggttgta
     1921 gtgcagctcc agttgtggat gtctcaagtg agacttggcg gcggcgagga gatcagccaa
     1981 ccgattggca gactccaata ggacttcgaa ctgactctct gcaacagccg tctccagtgc
     2041 tgtaaaggct tcagcgatta catccgcatt atcaaaaccc gcctcaatcg cttgattgac
     2101 cgtcagccag gtcatctctt ctctgtgctc tcctgtcgtt ggacagatga tggcaagggc
     2161 atcttgaggc ttggagagag agcgaaacaa ctcaacctgt tggaacagct cgagcaaggc
     2221 gcgttctttt tctgtcagtt cctttccctg ttccttactg gtgaaagcgc gtcgcagcgc
     2281 ctctcgaaac actttgtgtt ctgaaagcat cttgaaggac actcttcgag atgggaaatc
     2341 cagatcggaa cgaagagcag ccaaacgcac cttgataacg ggctcttctg accacgtttg
     2401 ccatttgagc atcatagaca gaaggaggga aacgggttga cccgacatgg ggaatccaga
     2461 acctttgaaa cttgcccaca cctcgttgcc agtgattgtt cgaagtgtct ctcgtgcaaa
     2521 tgtgtccaag ggtttcaggc gacccctgtc ctggatcgcg atatcatgca gtggtgcaag
     2581 aacggaagtc tcgccgcgaa tgatatcaac cgagacaagc aggatgaata aggatacggc
     2641 aagaagcggt gcgagaatct tcatccgttt tgagccgctc ctgttctctg acgtggcagg
     2701 taaaaggtca gtgcaatgcc aacaatgagc aagcaactcc cagagtacac gagcggaacc
     2761 cccggatcgc gggagacgga gagtacggag acgctggttc catcgggatc ctgatagtag
     2821 ctggactgaa acagtcgaaa cccattcatc acaagtggct gattcatgct gatcgtatgt
     2881 ttttcaacaa ggtcgggctt gtctgtcgga ctgacatcta ctgtgctgta ataacgagcc
     2941 gccatccccg ttccgccata ggtcggattg ccgaactcca gaagacgaat ctgaaaccca
     3001 agaggcactt cgcgttgtga cagtcgcag
//
LOCUS       DAOQUC010000343         3029 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_37825_length_3029_cov_2.132818, whole genome shotgun sequence.
ACCESSION   DAOQUC010000343 DAOQUC010000000
VERSION     DAOQUC010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3029
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37825_length_3029_cov_2.132818"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..682)
                     /locus_tag="PKH07_07035"
     CDS             complement(<1..682)
                     /locus_tag="PKH07_07035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34734.1"
                     /translation="MCTRTFCGVLAICFLAGSAAANTVIDYALDDGWTYEEGYPGTLS
                     LAANPSGGIIANVDQPVGIEDGGLVASRHDLPGYSLNNVGCIQLEYSALSSTITGEAG
                     LNLVLELEFYDAGGQEYEMAMAIWLTNGGAEFETWYGPNIAEEFYDTPVPDGLLLAEG
                     ALGLYSDGSHVLPYFKDVAGSVFYPFSTWDISGLVGMHRYSVDNDFEAETIDGGTVAA
                     SVNLERVVY"
     gene            complement(849..1283)
                     /locus_tag="PKH07_07040"
     CDS             complement(849..1283)
                     /locus_tag="PKH07_07040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34735.1"
                     /translation="MNKSRVSLLLVFFGLAGCAHGPMASVPSVSSPDEAAEVIVIRRK
                     SAVESPLSYIVMLDGQEIFRMKAGDYASFKVEPGTHSIGVEWLYSWPSKWREMTLIVA
                     CDPRTKYYFLVRPRGTMTIIEPISEQRGLELVSKSTYKPMTQ"
     gene            complement(1519..1824)
                     /locus_tag="PKH07_07045"
     CDS             complement(1519..1824)
                     /locus_tag="PKH07_07045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34736.1"
                     /translation="MVRREGGAYPAREKNYAIEVYYEDDVPPTSGVRIGCVQVLSHGQ
                     DMNAVKLEGELELKDLARGLGGDYVVAETPRVSRGGIYTYVCIRGLVYRARPAGGTE"
     gene            complement(1917..2366)
                     /locus_tag="PKH07_07050"
     CDS             complement(1917..2366)
                     /locus_tag="PKH07_07050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34737.1"
                     /translation="MSNYLCGRLCGAIVGLVAFSGCVTVPQRIGYHEDMQLDVPECRG
                     DNNCAVLLSENIKAVNPKLLLKVLGEIDAVVLHESSLYLYAVRREHAHILAPSEGGET
                     LSIHLKEITDGTTFVSIFTRLNEQGEGVDRWSKVVADRLAEEARDRR"
     gene            complement(2391..>3029)
                     /locus_tag="PKH07_07055"
     CDS             complement(2391..>3029)
                     /locus_tag="PKH07_07055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34738.1"
                     /translation="CDVRFSGDRYSSLVATLCFLLVAVASGCASRVVIPIRYSGVDDV
                     PSLDPPLRHLKVLVVGFSDVRKNPEVIGRAGSRRVHTTGNVPQLVTEAVVDALCKAGL
                     TVARQPVYDPASSRDYDVVILGRVVEFLATIKSGWSRLPCSTIVSVQCKVICKGGRES
                     EWTETVSGMDERKVESQVYFTEVRDFTDSALQACMRNLVAHLIENRLLECNF"
BASE COUNT          737 a          923 c          687 g          682 t
ORIGIN      
        1 catatacaac ccgttccaga ttcacggacg ccgccacggt ccccccatcg atggtttcgg
       61 cctcgaaatc attgtccacg ctgtatctgt gcatcccgac gagcccggat atgtcccagg
      121 tagaaaacgg atagaacacg gagcccgcta catccttgaa atacgggagc acatgcgagc
      181 catcgctgta gagccccaac gcaccctcgg caagcaagag cccatcggga actggcgtgt
      241 catagaactc ctccgcgatg ttcgggccat accatgtttc gaactctgct cccccattgg
      301 taagccagat cgccatggcc atctcgtact cctgtccgcc tgcatcgtag aactcaagct
      361 ccaagacaag gttgaggccg gcctcgccgg ttatcgtgga ggagagagcg ctatactcca
      421 actgaatgca tccgacattg ttcagcgaat accccggcaa atcgtgcctt gatgctacga
      481 gccctccatc ttctatgccg acgggttggt cgacattggc gatgattccg ccggagggat
      541 tcgcagccag cgaaagcgtt cctgggtacc cttcttcata ggtccagccg tcatccaatg
      601 catagtcaat gaccgtattg gcggctgccg atccggcaag gaaacagatt gccagaacac
      661 cacagaatgt tcgtgtacac atgtcattcc tccttctctc caagtcgttc gaatgccgta
      721 gcccacgtcg tcagacaatc gcttccttat atcatcctat gtccaagaac gcgccggcgg
      781 aacccagacc gaagatcaca agaaccagcc cgccaggggc gggcaccgtc acccccatct
      841 tcggcccgtt attgagtcat tggtttgtag gtactctttg aaacaagctc cagcccgcgt
      901 tgctctgaga tcggttcaat tatcgtcatt gtaccgcgag gccttacgag aaaatagtac
      961 ttcgttctcg gatcacacgc tacaatcaat gtcatttccc tccattttga aggccaggaa
     1021 tagagccact caacgccaat agagtgcgta cccggctcca ctttgaagct ggcgtagtct
     1081 ccggccttca ttcggaatat ctcctggcca tccaacataa ctatgtagct aagtggtgat
     1141 tcaacagcac tcttccttct aatcacgatg acttcagccg cctcatctgg agaagaaaca
     1201 ctgggtacgc tggccattgg tccatgagcg cagccggcca ggccaaagaa aactaagaga
     1261 agcgacaccc gactcttgtt catagctcaa tctcctttac tgcggattct ccttagggac
     1321 tgggtattca atcgtaagta gacagattcg gacaaaatac ggcggtctgg cttcccatcg
     1381 ccgcaacaca ccgccccgcc gcgtggcgat ccgagaagcg gcttcaggat tgataatgcc
     1441 gagacaacac acctgagacc ttcagttctt ttacaggtcg ccggggtgac ttcgacaacg
     1501 tcaatccacg gaggttccct attcggtgcc tcccgctggt cttgctctat acacaagtcc
     1561 tcgtatgcac acataggtgt atatcccacc acgcgacact ctcggcgtct cagcaactac
     1621 atagtcgcca cctaatccac gcgcaagatc cttcagttcc aactctcctt ccaacttcac
     1681 agcattcatg tcttgcccat gagacaacac ctgaacacac ccgatccgca cgccgctggt
     1741 cggaggaaca tcgtcttcat agtagacctc tatggcgtag ttcttctctc gcgccggata
     1801 cgcaccgccc tccctgcgca ccattgtatt cgattctggg ccacacccag acgccaacat
     1861 catgaacagg attggccaat tggacttatt agaacacaca ggattcgcac tacctactac
     1921 ctacgatccc ttgcttcttc ggcaagccga tctgcaacta ccttcgacca tctgtcgaca
     1981 ccttcacctt gttcgttcag tcgtgtgaag atactcacaa atgtcgtgcc atctgtaatc
     2041 tccttcaaat gtatggacag agtctccccc ccttcgctgg gtgcgagaat atgggcatgt
     2101 tcgcggcgca cggcatagag atacagggag ctttcgtgca aaacaaccgc atcaatttcg
     2161 cccagcacct tgagcaacaa cttcggattg accgctttga tattctcact taggagaacg
     2221 gcgcagttgt tgtcaccacg gcattctggc acgtcgagct gcatatcctc gtggtagccg
     2281 attcgttgtg gcaccgtcac acaaccggaa aacgccacca acccaacgat tgcaccacat
     2341 aatcgtccgc ataggtagtt gctcatgtta gcactccctt cacagatggt tcagaaattg
     2401 cattccagaa gtctgttctc gatgaggtgc gcaacaagat ttctcataca agcctggagg
     2461 gcagaatctg tgaagtcacg aacctctgtg aaataaacct gtgattccac ctttcgctcg
     2521 tccatccctg aaacggtctc agtccactca gattccctcc cccctttgca tatgaccttg
     2581 cattgaacag agacaatggt gctgcagggc agacgtgacc aacccgattt gattgtcgcc
     2641 agaaactcga ccactcttcc aagtatgacc acgtcatagt cccggcttga tgcaggatcg
     2701 taaactggtt gccttgctac ggtgagccct gctttgcaga gagcatcgac cacagcttct
     2761 gtcaccaact gcggcacgtt acctgtcgtg tgcactctcc gcgaaccagc tctgccaata
     2821 acctctgggt tcttgcggac atcgctaaaa ccgacaacca gcacctttaa gtgacgcaaa
     2881 gggggatcca gcgatggaac atcatccacg ccggaatagc gaattggaat aacaacacgt
     2941 gatgcgcagc cactggcgac tgcaacaagg agaaagcaca aggtggcgac aagagaagaa
     3001 tatcggtcgc ctgaaaacct cacgtcaca
//
LOCUS       DAOQUC010000344         3025 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_37898_length_3025_cov_3.006397, whole genome shotgun sequence.
ACCESSION   DAOQUC010000344 DAOQUC010000000
VERSION     DAOQUC010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3025
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37898_length_3025_cov_3.006397"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..889
                     /locus_tag="PKH07_07060"
     CDS             <1..889
                     /locus_tag="PKH07_07060"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNT34739.1"
                     /translation="LDSLPFPARYLVRNELYRRPDMDVPQTTIVTSRGCPFKCVFCLS
                     RVVAGTKPRTRAPENILEEIRECVERFGIRDFLFRSDTFTARRSWIMDFCKALRSSDL
                     RIRWSCNSRVDTIDTEMLSAMKSAGCWLMAFGLESGSQEILDVIHKGISKDQIRNALK
                     ECRRAGIRSSVYFLLGLPWETEKTFQETVQFACEISPDFIEFFYTYPFEGTELYDVCI
                     REGLLNPDELPSLAYSQPAYATGHFTLEQLAQQRPKALRTFYLRPGYILRTLSRARSL
                     KILANYVRYGWKQLNYLMR"
     gene            920..1786
                     /locus_tag="PKH07_07065"
     CDS             920..1786
                     /locus_tag="PKH07_07065"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNT34740.1"
                     /translation="MIIDAHNHPDWRHHDLNAFLKDMNQCRIDKTWLLSWESPQDEYD
                     PHAIPVITDPGPRGPIPFARCLSYAERCPERFILGFSPDPRRPEAIDQLQAMMDIYGV
                     RICGEFKFRLMFDNPDAIRLFRFCGERGLPVTIHLDYELQRPSKYPRPSYWYGGSHES
                     LERALELCPDTVILAHAPGFWARISDDDRYLQESYPKGKVIPEGKVVQMLRKYPNLYG
                     DLSGGSGHNALARDPEFGREFVLEFQDRLLYGRDLFDNRHQEVLQVMDLPDDVLEKVF
                     SGNALRLVPDKG"
     gene            1880..>3025
                     /locus_tag="PKH07_07070"
     CDS             1880..>3025
                     /locus_tag="PKH07_07070"
                     /inference="COORDINATES: protein motif:HMM:NF020120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-rhamnosidase N-terminal
                     domain-containing protein"
                     /protein_id="HNT34741.1"
                     /translation="MAAAKKSGIKPVYLRCEYKVNPLGIDVKKPRLSWELSSCNPDRH
                     GQRQIAYEIQAATSKSLLTKGQPDLWDSGMLRSDATNQITYRGRALKSHMECWWRVRV
                     SDEQGATSPWSEPAYWSMGVLNATDWKARWIGYDKPVPRPEIPAEMESQLSLSACRWV
                     WFPEGNPADEAPVGSRYFRRTLSIPDGVKIRSASFLLTADDQFTLYVNGVQAGKSDGK
                     RDAWKRPRTLVVADSLRCGINTLAIEATNTTVSPAGLAGVLVVLLDNGDSVVQRINRE
                     WKCNNEILSGWHQSDFDDSQWPNAAELGDMGRPPWGLPGKSEALHLPPPPYLRKSFVV
                     QKAVKRVVVYASALGLYELRINGEKVGNDYFTPGWTDYRKRVYYNTYD"
BASE COUNT          713 a          713 c          842 g          757 t
ORIGIN      
        1 cctggactca ttaccattcc ctgcgcgata cctggttcgc aacgagttgt acagaaggcc
       61 ggatatggat gttccacaga cgacaatcgt gacgagtcgg ggatgtccat ttaagtgtgt
      121 gttctgcctc tctcgggtcg ttgcaggaac gaagcctcga acccgtgcgc ccgaaaacat
      181 cctggaggaa atccgtgagt gtgtggagcg ttttggcatc agggactttc tgtttcgttc
      241 ggatacattt acggctcgtc gctcctggat catggacttc tgcaaggcac ttcgctcctc
      301 ggacttgagg attcgttggt cgtgcaacag tcgtgttgac actatcgaca ctgagatgct
      361 ctcagccatg aagagcgccg gttgttggct gatggctttc ggtctggaga gtggcagtca
      421 ggaaatcctg gatgtcatcc acaagggaat atcgaaggat cagattcgca atgcgttgaa
      481 ggaatgccga cgagccggta ttcggagcag cgtttatttt ctgttgggat tgccttggga
      541 gacagaaaag actttccagg aaacggttca attcgcatgt gagatatcgc ctgatttcat
      601 cgagttcttt tacacatatc cctttgaagg gacagaactt tacgacgtat gtattcgtga
      661 agggcttctg aatcccgacg aacttccctc tcttgcgtat tcgcaacctg cgtatgcgac
      721 agggcatttc actctggagc aattggctca gcaaagaccg aaggcgctga gaacgttcta
      781 ccttcgtccc ggctacatct tgagaacatt gtcgcgtgct cgttccctga aaatactggc
      841 caattacgtt cgatacggat ggaagcaact gaattatctg atgcgataga tatcttgatg
      901 cgtctatggg agggattgca tgattattga tgctcacaat catccagact ggcgacacca
      961 tgatttgaac gcctttctca aggacatgaa tcagtgtcga attgacaaga cttggctact
     1021 cagttgggaa tcacctcagg acgaatacga tccccacgca attcctgtga tcaccgatcc
     1081 aggtccccga ggcccaatac cctttgcgcg ctgcctcagc tatgcggaac gatgtccgga
     1141 acgtttcatc ctgggatttt cacctgaccc cagaagaccg gaagctattg accagctcca
     1201 agcaatgatg gacatctacg gcgttcggat ctgcggcgag ttcaagtttc gtcttatgtt
     1261 cgataacccc gacgccattc gtctgtttcg tttctgtggc gaaagaggtc tccctgtaac
     1321 cattcatctg gactatgaac tgcagaggcc gtcgaaatac ccccggccaa gttattggta
     1381 tggggggagt catgagtcgc tggaacgcgc attagaactc tgtcctgaca cagtcattct
     1441 ggcacatgct cctggattct gggcacgcat atcggatgac gatcgttacc tgcaagagag
     1501 ttaccccaaa ggcaaagtga ttccagaggg caaggttgtc caaatgctcc ggaaatatcc
     1561 aaatctctac ggcgatcttt ccggtggatc tgggcacaat gcacttgctc gagatcctga
     1621 gtttgggcgt gagtttgttc tggaattcca ggatcgtctg ctttacggca gggatttatt
     1681 tgacaaccgt catcaggaag tccttcaagt aatggatctt cccgatgatg tcttggaaaa
     1741 ggtctttagc gggaacgcgt taagactcgt tcctgacaaa ggatgaagag atttgaatcg
     1801 cactgagaac agtaagcgga tctgggtcgc acttgcggca tttgtcctct tatgttttct
     1861 gggtagggga gaagaaacta tggctgctgc aaagaaatca ggaatcaagc cggtctactt
     1921 gcgatgtgag tacaaggtca atccgttggg cattgatgtg aagaagccac gcctgagttg
     1981 ggaactgtct tcgtgcaatc ctgatcgcca tggtcagaga cagatagcct atgagattca
     2041 ggcagcgacg agcaagtcct tactcacaaa ggggcaaccg gacttgtggg attccgggat
     2101 gctgagatct gatgccacta accagataac ctatcgggga agggcgttga agtcccacat
     2161 ggaatgctgg tggcgagtga gagtatcgga tgagcagggt gcaacgtctc catggagtga
     2221 accggcctat tggagtatgg gcgtgctgaa tgcgactgac tggaaggctc gatggatcgg
     2281 ctacgataaa cctgttccga gacccgagat tcctgcggag atggagtcgc agctgagtct
     2341 tagtgcatgt cgctgggttt ggtttccgga ggggaatccg gctgacgagg cacctgttgg
     2401 cagcaggtat ttccgacgca ctctttcgat ccccgatggc gtgaaaatca ggtcagcctc
     2461 atttctgctg acggccgacg atcagttcac cctttatgtg aatggtgttc aggcgggaaa
     2521 gagcgatgga aagcgcgatg cctggaagag gccaaggaca ctggtcgtgg cagattctct
     2581 gagatgcggt atcaacacgc ttgcaattga ggcgaccaat accacggtca gcccagctgg
     2641 tttggctggc gtgctggttg ttcttctgga caatggtgac tcagtcgttc aaaggatcaa
     2701 ccgagaatgg aaatgcaata acgagatctt gtccggctgg catcagtctg attttgatga
     2761 ttcccaatgg ccaaatgcgg cggaacttgg cgatatggga cgtccacctt ggggacttcc
     2821 tggcaagagc gaagcgcttc atctgcctcc gccgccatac ctgaggaaga gttttgtcgt
     2881 tcaaaaagcg gtaaagagag tggttgtcta cgcatcagct cttggtctct atgagctgcg
     2941 tatcaacggc gagaaggttg gcaacgacta ttttacccct ggatggactg actacaggaa
     3001 gcgtgtttac tacaacacgt acgat
//
LOCUS       DAOQUC010000345         3019 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_38004_length_3019_cov_2.725709, whole genome shotgun sequence.
ACCESSION   DAOQUC010000345 DAOQUC010000000
VERSION     DAOQUC010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3019
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38004_length_3019_cov_2.725709"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            201..1268
                     /locus_tag="PKH07_07075"
     CDS             201..1268
                     /locus_tag="PKH07_07075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34742.1"
                     /translation="MLTDEELASEQAGETMIDFYGRVVDQHGDPVVGAQISVGMLHIA
                     KPPVREERVVETDLSGEFVIHWGKGSFLVINKIQRNGYEIGPKMYQYSYRSNWGYRHI
                     PDESNPVVFKMRKRAAEPTFLFHHIAADLKFDESNGLHTGFDVIRSYVDPDHRVLQRF
                     RDLEALALWEPGDATCEITLKANGDDTAGLILSDELLGLAPESGYQESLTLTFEDRKP
                     VQQKYLYVRSRTPAIYSRFEIGGAGIGRDWLELGLQGHTNPYGERNLEMDEQFPGAAS
                     SHIEAMVRKAYRDNVSLDVAPLKELIEVAKGTTKEESLRISREARDLEDKVEREERRN
                     FEIDTATLTEMYRKAKKVQER"
     gene            1632..2870
                     /locus_tag="PKH07_07080"
     CDS             1632..2870
                     /locus_tag="PKH07_07080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34743.1"
                     /translation="MNRIEVILLGIACMCATVTVPGVTDYAESQKSYSQRLQEESSRV
                     AAEEEPIEFYGKVIDHHGDPVCGATVLIQVGGASMGKRVQKAQVLTSLDGCFEYHSER
                     ASGLRIEEIAKPGYEFRRYQGMLCEFRYRKDYNPRHIPDRDSPVVFRVCKMDAEPTYL
                     LHDPFSLGGIVLDEKRSYQWGYDAIKPFVISSDFERGKVDDIHISVSRLESSTKCIFT
                     LTAEGGEEAGVYPSNEFLGMAPDSGYEPHYTFTAVRCPTGTLIIGPTGLQKILSMPLD
                     LSGMHFYIRSRMPFLYSHFHIQGISVGSKSIRLVAQIYTNPLGDRNLEMDEQFPGAAS
                     SHIEAMVRKAYPDGVPLDVVPLKELIEAAKGTTKEESLRISREVRDLEDKVEREERRN
                     FEIDTAMLTEMYLKAKKVKN"
BASE COUNT          794 a          687 c          833 g          705 t
ORIGIN      
        1 agtttctatc gaacgtcgta gatgaatggc tctactggta ttggctgcgg aggcacgcga
       61 tgcttccatg caccatgcct attcattgat tgtagtagca aaggagattt gtcgtgacta
      121 aaacagaccg tgtatcgaaa cttgtatggg tctgcttatt gttactcgga acagctgggg
      181 cgtttgcaca agaggaattc atgctgacag atgaggaact cgcaagcgag caggcgggag
      241 aaaccatgat tgacttctat ggcagagtgg ttgaccagca tggcgatcca gttgtgggag
      301 cacagatatc tgttggcatg ttgcacattg caaagccgcc cgttcgggaa gagcgagttg
      361 ttgaaacaga tttgtctggc gagtttgtca ttcattgggg caagggctct ttcttggtga
      421 tcaacaagat tcaaaggaat ggctatgaga tcggaccaaa gatgtaccag tatagctacc
      481 gttccaactg gggataccgt cacatccccg acgagtccaa tccagtcgtt ttcaagatgc
      541 ggaagagggc ggcagagccg acctttctct ttcaccatat cgctgctgat ctgaaattcg
      601 atgaatccaa cggccttcat acgggcttcg atgtaatcag gtcctacgtg gatccggatc
      661 atcgcgtcct gcaaaggttt cgcgatcttg aggctttggc gctttgggag cctggtgacg
      721 caacctgcga aatcaccttg aaggcaaacg gagacgacac agcagggctc atcctgagcg
      781 acgagttact cggcttggct ccggagtcag gttaccaaga gtcactcact ctcacttttg
      841 aagacaggaa accggtccag cagaagtacc tttacgttcg tagcagaact ccggcgatct
      901 acagtcgttt tgagatcggc ggtgcaggga taggcaggga ttggctggag ctcgggctcc
      961 aaggacatac caacccctac ggcgagcgca acctggagat ggacgagcag tttcccggag
     1021 cagcctcaag ccatattgaa gcgatggtaa gaaaagcgta tcgtgataat gtttctcttg
     1081 acgttgcccc tctgaaggag ttgatcgaag tggcaaaagg aacaaccaag gaagaaagtc
     1141 ttcgtatctc ccgagaagca cgtgatctcg aagacaaagt cgagcgcgaa gagagaagaa
     1201 acttcgaaat agatacggca acattgacag aaatgtaccg caaagcgaag aaggttcagg
     1261 agaggtaggg cagtcgagta gggtggaact cctggactcg ttgccaccct atccacttgg
     1321 agtggccgga attcccgccg gacgcccctg cagtggttgt ggaggacgga gacttggtgt
     1381 tccccgatga cacgcatgag atattcagct atgctgcgga agcacgaagc aaagcacttg
     1441 gcgctcagac cgagctaaca tatttttctg atttcgatct ccaatcgtcg ccgctcaatt
     1501 atgatgaaaa ccactactct cacagccgcc agtttcgctc gaatatcatc gacgaaaaga
     1561 catactggtc gtttgtgcat gagtgcttat ctctatctga gtgattcagt cccattagga
     1621 aaggaataaa catgaatagg atagaagtga tcttgctagg aatcgcctgc atgtgcgcca
     1681 cggtcactgt gccaggagtg acagactatg ctgagtcgca gaagtcttat tcacagcgac
     1741 tgcaggaaga gagtagtcga gttgcggctg aagaggagcc catagagttt tacgggaaag
     1801 tcattgatca ccatggcgac ccggtctgtg gcgctactgt gctgattcaa gtcggtggtg
     1861 cttctatggg gaagagagtc cagaaagcgc aggtacttac ttcactggac ggttgctttg
     1921 aatatcactc cgaaagggca tctgggcttc gaatcgaaga gatagccaaa ccaggctatg
     1981 agttccgccg gtaccaaggc atgttgtgcg aattcaggta tcgcaaagac tacaacccaa
     2041 ggcatatacc tgacagggac agccctgtag tgtttagagt ctgcaagatg gatgctgagc
     2101 cgacctactt gctacacgat cccttctcat tgggggggat tgtccttgat gagaagcgaa
     2161 gttaccagtg ggggtacgat gccatcaaac cttttgtcat ctccagtgat ttcgagcgag
     2221 gaaaggttga tgacattcac atttcagttt cacgcttgga gagtagtacg aagtgcattt
     2281 tcacattgac ggctgaaggc ggtgaagaag cgggcgtgta cccaagcaac gagttccttg
     2341 gcatggctcc agattcagga tacgagccac actacacttt tacggcagtg agatgtccga
     2401 caggcacgct gattatcggc ccaacaggtt tgcagaaaat tctgtccatg cctctggatt
     2461 tgtcaggcat gcacttctat atccgaagcc gaatgccctt cttgtactcg cattttcaca
     2521 ttcagggaat cagtgtgggt tcgaaatcca ttagactggt tgcgcaaatc tacaccaatc
     2581 ctctcggcga ccgcaatctg gagatggacg agcagttccc cggagcagcc tcaagccata
     2641 ttgaagcgat ggtgagaaaa gcctaccccg atggtgtgcc ccttgatgtt gtgcctctta
     2701 aagagttgat cgaagcggca aaaggaacaa ccaaagaaga gagtcttcgg atctcccgag
     2761 aagtacgtga tctcgaagac aaggtcgagc gcgaagagag aagaaacttc gaaatagata
     2821 cggcgatgct gaccgaaatg tacctcaagg cgaagaaggt caagaactga gagcagtgcg
     2881 gcattcatgc cgcgaaaagg agcgggagca tcttgctccc gactggactt tggccattac
     2941 tagatttttc gcaagttttc tcgtgtgaag tgattttgat gttgcatttg agaggggaag
     3001 aatgatagat ttatatcta
//
LOCUS       DAOQUC010000346         3017 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_38037_length_3017_cov_3.570223, whole genome shotgun sequence.
ACCESSION   DAOQUC010000346 DAOQUC010000000
VERSION     DAOQUC010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3017
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38037_length_3017_cov_3.570223"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1086
                     /locus_tag="PKH07_07085"
     CDS             <1..1086
                     /locus_tag="PKH07_07085"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HNT34744.1"
                     /translation="HLEPQEDTLRIRYRIDGMLYDFMTMPKERSVPVISRLKILAGMD
                     ISERRRPQDGHISMQADGREIDLRLATLPTVLGEKIVLRVLEETSVLKGLNQLGFESD
                     DLEKVRKAVHAPNGMVLVTGPIGSGKTTTLYSALTEVNVQTRNIVTIEDPVEYQLPGI
                     NQVQVDPKINLTFAEGLRAVLRQDANVLMVGEIRDVETAKVAVRAALTGHLLLSTLHT
                     NSALGAISTLYHLGIEPILLSSAVSCVIAQRLVRKVCQQCCQMRLPAAGLRSQLGLPA
                     SSKKKIPYAKGCKECFHTGYSGRTAVYEVICVSDNLREMILQGASESAMIEAAKKDKS
                     SFLLENGKKKVLEGITTFEELVRTIYM"
     gene            1146..2243
                     /locus_tag="PKH07_07090"
     CDS             1146..2243
                     /locus_tag="PKH07_07090"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNT34745.1"
                     /translation="MADEQNKNSAAGSTHKKMQFRGASRREIAAFARELSVLMDVGMP
                     LLKALRILARRVSNHDLSTVISDVAASIEKGSTLSDALSRHPKLFDGLFINVVRVGEV
                     GGVLDQSLRRLANFLEEQARIKNKVFYALMYPCTVVFLSLVALCVIVGWVVPQFMAAF
                     PDVSKLPTPTRLVYGWGLFLESYWWLVLIALIVLVFLFKVWKKTAGGRQVVDGLKLRL
                     PIFGRFGTKVVTERVTTTFATLLRSGIQILDALKIVGNTADNVVVEQAFLEAAAKVER
                     GENLAESLEKANIFPPTVVDMIAIGDEAGALDVVLEKVSTMLKEEIDYALDGLTSLLE
                     PVLIIVLGIVIIFIALSVYLPYWKLGQQVFG"
     gene            complement(2444..2983)
                     /locus_tag="PKH07_07095"
     CDS             complement(2444..2983)
                     /locus_tag="PKH07_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HNT34746.1"
                     /translation="MTPEPTAEIIARALRIKLLCLDVDGVMTDGRIVFDSDGAESKFF
                     HVQDGSGIRYAQRAGLTVAFVTGRESPVVGRRAEELGVSLLYQKVKQKLPVVEELLSR
                     LGLTFEETLCMGDDLPDFPVMRRAGLAVAPANAVQEIKEVAHLVTDRSGGHGAVREMI
                     DWLLKTNGTWQGIMERYLK"
BASE COUNT          691 a          769 c          859 g          698 t
ORIGIN      
        1 cacctggagc ctcaggaaga cacgcttcgc atccgctacc gaattgatgg aatgctgtac
       61 gacttcatga ccatgcccaa ggagagaagt gtacctgtca tttcacggct gaaaattctt
      121 gctggtatgg acatcagcga gcggcgacgt cctcaggacg gtcatatttc catgcaggca
      181 gatggacgtg agattgatct tcgactcgcc acgcttccca cggtgctggg cgaaaaaatt
      241 gttctacgtg ttctggagga gacctctgtt ctcaagggtc tcaatcagct cgggtttgag
      301 agcgacgacc tggagaaagt tcgaaaagcg gttcatgcac ccaacggaat ggttctggtt
      361 acaggtccca tcggaagtgg taagacgacg accctctact cggcattgac cgaggtcaat
      421 gtccagactc gcaacattgt gaccattgag gacccggtcg agtatcagct tcccgggatc
      481 aaccaggtgc aagtggaccc gaagatcaat ctgacctttg ccgaaggact tcgagcggtg
      541 ctgaggcagg atgccaacgt gctcatggtg ggtgagattc gagatgttga aaccgccaag
      601 gtggctgttc gtgctgcact gaccggtcat ctgctgctca gtacacttca caccaacagt
      661 gcattgggtg ccatttcgac gctctatcat ctgggcatcg aaccgattct gctctcgagt
      721 gcggtcagtt gtgtgatcgc ccaacggttg gtgcggaagg tctgtcagca atgttgtcaa
      781 atgaggcttc ccgcggcggg actcagaagc caactgggtc ttcccgcaag cagcaagaag
      841 aagattccct acgccaaagg ctgcaaggaa tgcttccata caggctacag cggccgcacg
      901 gcggtctatg aagtgatttg tgtttctgac aacctgaggg agatgattct tcagggcgct
      961 tcggagtctg ccatgatcga ggcagcgaaa aaggacaaaa gcagtttcct gctggaaaat
     1021 ggcaagaaga aagttcttga aggaatcacg accttcgaag aactggttcg aaccatttat
     1081 atgtaaagaa accataggaa caaagtagta cagctgaatt tcagatggta ttgaggaggt
     1141 tggcgatggc ggacgaacag aacaaaaatt ccgcggctgg ttccacccac aagaagatgc
     1201 agtttcgagg ggcaagcagg cgggagatcg cggcgtttgc tcgggagttg tcggttctca
     1261 tggatgttgg gatgccactg ctgaaagcgc tgcggattct agcccggcgg gtcagcaatc
     1321 acgatctctc gacagtgatt tcggatgtcg cagccagcat cgagaaaggc tcaacacttt
     1381 ctgatgcctt gtcgagacat ccgaagcttt ttgatggtct cttcatcaat gtggtcagag
     1441 tgggagaagt cgggggcgtc ctcgatcagt cccttcgacg cctggcgaac ttcctcgagg
     1501 agcaggcgcg catcaagaac aaagtcttct atgctttgat gtacccctgc acggtggtct
     1561 ttctgagtct cgtggccttg tgcgtcatcg tgggatgggt cgtgcctcag ttcatggccg
     1621 cgtttcctga tgtcagcaag ttgccgaccc cgactcgcct ggtctatgga tggggacttt
     1681 tcctcgaaag ttactggtgg ctggttctga ttgcccttat tgtcctggtg ttcttgttca
     1741 aggtttggaa gaagaccgca ggtgggcgac aggttgtgga tggactgaaa ttaaggctcc
     1801 ccatcttcgg tcggttcggc acaaaagtcg tcacggagcg agtgacgaca accttcgcca
     1861 ccctgttgcg cagtgggatt cagattctgg atgctttgaa gattgttgga aacactgcgg
     1921 acaatgttgt tgtggaacaa gcgttcctcg aagccgcggc caaggtcgaa cgtggggaga
     1981 atcttgccga gtctctggag aaggcgaaca tattcccacc cacggttgtg gatatgatcg
     2041 caataggcga cgaagcaggt gctcttgacg ttgttcttga gaaagtctcc acgatgctca
     2101 aggaggaaat tgactatgct ctggatggtc ttacctctct cttggagccg gttttgatca
     2161 tcgttctcgg tatcgtgatc atcttcattg ccctgtccgt ctatctgcct tactggaaac
     2221 tcggacagca ggttttcggc tagagctgga cgacagacga atcacgtggt cacatcaact
     2281 gagggagagg aaagcctctc cctctttctt tccaaacaga gggggcgtag cgcaagagtt
     2341 gaatgctcgt tttatgcctg agaggacgga tccgaatcac gccttacgac gcaactgcag
     2401 acccagagaa gaactcgcat cgggagaatg cgagatagaa gactcacttc agataacgtt
     2461 ccatgatgcc ttgccaggtg ccgttggttt tcagcagcca gtctatcatc tcacgcaccg
     2521 caccatgtcc gccagagcga tcggtgacca ggtgcgccac ctctttgatc tcctgcacag
     2581 cgtttgccgg agcgacggca agacccgcgc gacgcatgac cgggaaatcc ggcagatcat
     2641 ctcccatgca gagagtttct tcaaacgtca acccgagcct gctgagcaac tcctcaacga
     2701 ccggcagctt ctgcttgacc ttctgataca gcagcgaaac gccgagttcc tctgctcgtc
     2761 ggccaacgac gggggactct ctgccagtga caaaagccac cgtgagaccg gcacgttggg
     2821 cataccggat gccgctccca tcctgaacgt ggaagaactt gctctcggct ccatctgagt
     2881 caaagacaat tctgccgtcg gtcatgacac cgtccacgtc caggcagagg agtttgatac
     2941 ggagcgcacg ggcgatgatc tcagcggttg gttcgggggt cattaccgac ggcagaattg
     3001 tctttgactc agatgga
//
LOCUS       DAOQUC010000347         3010 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_38168_length_3010_cov_3.879865, whole genome shotgun sequence.
ACCESSION   DAOQUC010000347 DAOQUC010000000
VERSION     DAOQUC010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3010
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38168_length_3010_cov_3.879865"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..527)
                     /locus_tag="PKH07_07100"
     CDS             complement(<1..527)
                     /locus_tag="PKH07_07100"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNT34747.1"
                     /translation="MSRLLIVDDDPLNRALLEEELGDSGYRTFLAEDGDTALQILEEK
                     NIDLVLLDIMMPRMSGLDVLAKIRESHSVADLPVIMVTAKHDSEDIVQSLNQGANDYI
                     VKPIDFGVLQARIRTHLTMKSLSQEKDHFISIASHDLKNSLTTIIGAARVIGAMLPSN
                     DTSLETVRGMVANIH"
     gene            complement(773..>3010)
                     /locus_tag="PKH07_07105"
     CDS             complement(773..>3010)
                     /locus_tag="PKH07_07105"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNT34748.1"
                     /translation="ELELRRQDWTTWWASVTVTALRDARGDIIYIDGTMQDITRLKHV
                     EDALREQIERNELILHRSMDGFLLMNINGKILDVNPAFSRIVGYSRAELIGRSISDLE
                     DPNAAVLLTQNLEEICSKGSDRFETRFLRRSNLGIDLEISANFANVGDTDFVFCFFRD
                     ISERKKAEAEIRHRLSIEEALAKVSALFVSSGSLDLQEILAILATALGTSRAFLVLVN
                     DTDGRVLSAAEWCDSSVSPPAVSVLGSCLLSCSTMVDRLRCDENLVFSDVLELDSETS
                     RERDFLIGQNVGALLSVPVKQTEQHLGGLIGVVSQQAPRYWSEADAQALRVAGEMAFR
                     YVERRQADAALRESEEKYRLLVENVNDGIVIGQNDRFVFINRRFAEMLGYEPEELLMK
                     DYRKIYTPAAVERLWTRRQSRDLGESVPERYETVFRRKDGTEMDVETSVTIIDYKGEL
                     ATFGVVRDITERKNAEREFRNHQRELEQLTSELAAARDRALAASRAKSAFLANMSHEL
                     RTPLNAIIGFCRIVIRKTRDSIPEQQIQNLQRVLVSANHLLALINDILDLSKIEARRM
                     PVNVDSLDIPALIHEVIGTVHSLLESNRNRLVVNVAGNLPRIRSDRTKVWQILLNLLS
                     NAAKFTQNGTITVSVWVIDRVSAHLPELAMDQEGQLAIQVQDTGIGMSEEHLRHIFEE
                     FWQVDDSTTRQHGGTGLGLTVSRRLAHLLGGTISAESQLGHGSRFTLKLPLTTPPTLA
                     DDEFE"
BASE COUNT          696 a          856 c          744 g          714 t
ORIGIN      
        1 tcgtggatat tggcaaccat gccacgcacg gtctccaaac ttgtgtcgtt cgaagggagc
       61 atcgcaccaa tcactcgggc ggcaccgatg atcgtggtca gagagttctt caggtcatgg
      121 ctggcaatgc tgatgaaatg atccttctct tgtgaaagag atttcatcgt caaatgcgtt
      181 cggatgcgag cttgcagcac cccgaaatca atgggcttga caatgtagtc gtttgcccct
      241 tggttcagtg actgaacaat gtcctcggag tcgtgcttgg ccgtcaccat gatgaccggc
      301 agatccgcga cggagtggga ctcccgaatc tttgcgagca catccaagcc gctcatgcgc
      361 ggcatcatga tatccaacag caccaggtct atgttctttt cttcgagaat ctgaagtgcc
      421 gtgtccccgt cctcggccag aaacgttcga tatccagagt cccccagttc ttcttccaac
      481 aaagcccgat ttagcgggtc atcgtccaca attaagagcc tgctcatcct tgtcctattc
      541 tttcctccgt gtcatcaaac tgggtcttga gaccctttga agtcattcca aatcccagga
      601 acccatgttc caagatgccg ccccaagtcc cgcaaaggtt ctctcttcgt atctcgatca
      661 agagccgaag agattattcc ctgcgttcct aagaagccgg actctagagg aaatggtttc
      721 ctataccaac aggcgcctcg cataggtggg cagagcccgc atgccggcca tatcactcga
      781 attcatcatc agccaaagtt gggggtgttg tcaacggcag tttgagtgtg aaccgagatc
      841 cgtgtcccaa ctggctctct gcagagatag tccccccaag cagatgagcc aggcgtctgc
      901 tgactgttag acccaatccg gtacctccat gttgtcgagt cgtggagtca tccacctgcc
      961 aaaattcctc gaaaatgtgt cgcagatgct cctccgacat tccaatgcct gtgtcctgta
     1021 cttgaatggc cagctgtcct tcctgatcca tggccaactc cggaaggtgt gcggaaaccc
     1081 tatcgatcac ccagacggag acagtgatgg ttccgttctg ggtgaacttg gccgcgttgc
     1141 tgagaagatt caggagaatc tgccagacct tagtcctatc ggaacgaatc cttgggagat
     1201 tcccggcaac gttgaccacc aatcggtttc ggttggactc cagcaggcta tgcacagtgc
     1261 caatcacttc gtggatgagt gcagggatat ccagggagtc cacattcaca ggcattcgcc
     1321 tggcttcaat cttggacaga tcgaggatat cattgatgag tgccagcaaa tggttcgcgc
     1381 tgaccagcac acgttggaga ttctgaatct gctgttctgg aatggagtct cgagtctttc
     1441 gaatcacgat acgacaaaat ccgataatgg cattgagcgg ggtgcgaagc tcatgagaca
     1501 tattggccag gaaggcggat ttggcgcggc tggctgccag cgcgcgatct cgcgccgccg
     1561 ccagttctga ggtcagctgc tccaattccc tctgatgatt cctgaactcg cgttcggcgt
     1621 tcttgcgttc ggtgatgtct cgaacaacac caaaggttgc cagttctccc ttgtagtcaa
     1681 tgatcgtgac actggtttcc acgtccattt cggtgccatc cttgcgacga aaaaccgtct
     1741 catagcgttc cggaacagac tcaccaagat ctcggctctg tcttcttgtc cagagacgct
     1801 ccaccgcggc aggcgtgtat attttgcgat agtccttcat caagagttct tcaggttcat
     1861 agcccagcat ctcggcaaag cgacggttga taaacacaaa gcgatcgttt tggccaatga
     1921 caattccatc attgacattc tcgaccagca ggcgatattt ctcctcgctt tctcgaagag
     1981 cagcgtctgc ctggcgccgt tccacatatc gaaaggccat ttccccagcc acgcgaagcg
     2041 cctgggcatc ggcctccgac cagtagcgtg gtgcctgttg ggataccact ccgatgagtc
     2101 ctcccaagtg ctgttcagtt tgttttaccg gaacgctgag caatgcccct acgttctgtc
     2161 cgataaggaa gtctctttcc cgagaagttt ctgaatccag ttccaaaaca tcggagaaga
     2221 ccaggttctc gtcacaccgc aaacggtcaa ccattgtgct gcagctcagc agacaactcc
     2281 ccagaacgga gacagccggt ggagatacgc tcgagtcgca ccactctgcg gcactcagaa
     2341 ctcgtccatc cgtgtcgttc accagaacca aaaaggcgcg tgaggttcca agcgctgttg
     2401 ccaaaatggc gagaatctcc tgcaaatcga gactgcccga ggaaacaaac aacgccgaga
     2461 cctttgccaa agcctcctcg atggagaggc ggtgacgaat ctccgcttcc gcttttttgc
     2521 gctcagagat atctcgaaaa aagcagaaaa cgaaatcagt gtcacccaca ttcgcgaaat
     2581 tggcgctgat ctccagatcg atgcccagat tcgagcggcg caggaaacgc gtctcaaaac
     2641 gatcggaacc ctttgagcaa atctcctcca ggttctgcgt caacaggact gccgcattgg
     2701 gatcttccag atcagagata cttcggccaa tcaactccgc tcgagagtaa cccacgatcc
     2761 ttgagaaggc tggattcaca tcaagaatct tcccattgat attcatgaga agaaatccgt
     2821 ccatagagcg atgcaggatc aactcatttc gctcgatttg ctcacgcagt gcatcttcga
     2881 catgtttcag ccgcgtgatg tcctgcatcg ttccgtctat gtagatgatg tctccacggg
     2941 cgtcccgaag tgcggtcacg gtgactgacg cccaccaggt tgtccaatcc tgtctgcgga
     3001 gttccagctc
//
LOCUS       DAOQUC010000348         2999 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_38400_length_2999_cov_2.899117, whole genome shotgun sequence.
ACCESSION   DAOQUC010000348 DAOQUC010000000
VERSION     DAOQUC010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2999
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38400_length_2999_cov_2.899117"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1636
                     /locus_tag="PKH07_07110"
     CDS             <1..1636
                     /locus_tag="PKH07_07110"
                     /inference="COORDINATES: protein motif:HMM:NF036710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbohydrate-binding protein"
                     /protein_id="HNT34749.1"
                     /translation="LSANIYGVHNYAGDLVATLKNGKGFYTVDMIVSEAWANDAGYRS
                     GYTNTVREAFNNGFTVILRIDYQVNQVCPPENDWVGRYHFGERCKDIVQTLKDYCRIW
                     IIGNEMQAGYANPISKEWYTTCFAGDTNGCYDKIHSVQSDAIVCFGGLGGWPGLKSEP
                     DSGVNYLDYFCKNCVGKVDGFTVHSYGGPANQALGGSLDDPRTSDIGSFGAYKAFCEV
                     IYENFRGSKPVYITESNTYWWIASPHPPHSMDSYQAGWIQKAFQAIDQWNQASDLKVH
                     ALCWYSYAHYGVCDGNAGDFWHNAIQPGRCPSDPELDQARSDYQWTTTNTDYRWDIPS
                     DGTLKIQVENYNNSVLWGDTIATGWEGTDYHDSEPANQGGGYRPDGVDIVENGDLIYV
                     GWTEAGEWLRYTVAGGKNTWYRVDLRYARQVAGNGQCRILVDGVNATGTILCPSTGGW
                     ESFSTASSPAFSLTDGVHDVRLLFDGNYLNADWFAFVPVEEPTATPTRTPTPTVSPTR
                     TPSPTSTQTNTPTPTRTATPTPMPTAPASASNWMGY"
     gene            complement(1676..2404)
                     /locus_tag="PKH07_07115"
     CDS             complement(1676..2404)
                     /locus_tag="PKH07_07115"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HNT34750.1"
                     /translation="MFHEGKVSEEDYLRLSRAMARNPKQPETPEETVAKRGRLRKSWS
                     NGVVGGLCAGLAEYLNVDVVVVRGIVIAAAILLSPLLILLYIILCSLTPWDDEQSAKA
                     LERTRETRLLVGTVAVFVLVIPTLYSLLVFPRLQEMYSNLGLSIWSANYQSSLPGRAI
                     DCVSEYAGYVRLMGRAQPDEWLILGLLFLVAVFSLALIGATYSCFCKERMRKNFARAT
                     IGLSVLWFAFLVLGTLMPLLGIQR"
     gene            complement(2449..2808)
                     /locus_tag="PKH07_07120"
     CDS             complement(2449..2808)
                     /locus_tag="PKH07_07120"
                     /inference="COORDINATES: protein motif:HMM:NF021388.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2089 family protein"
                     /protein_id="HNT34751.1"
                     /translation="MSDRKQTDCPYCKTPMRPVTMACDHCGVEIRGRFRQTRFAQLSD
                     ADSEFLERYLLAGFSIKALAEESGFGYVAIRSRLDRLIERYQKLRDDEDAKRAILDRL
                     EKGEITPSEAAEAIERL"
BASE COUNT          738 a          792 c          790 g          679 t
ORIGIN      
        1 tctttcggcg aacatctacg gtgtccacaa ctacgcgggt gacttggtgg cgacgctcaa
       61 gaacggcaag gggttctata cggtggacat gattgtaagt gaggcgtggg caaatgacgc
      121 cggttaccga agtggctaca ccaatacggt gagagaggct ttcaacaacg gtttcacagt
      181 cattctacgc attgactatc aagtcaatca ggtctgtccg ccggagaatg attgggtcgg
      241 gcgataccac ttcggagagc gctgcaagga cattgttcaa acactgaagg actattgccg
      301 catctggatc atcggaaatg agatgcaggc gggttacgcc aatcccatat ccaaggaatg
      361 gtacacgacc tgttttgctg gagacaccaa tggctgctac gacaagattc acagcgttca
      421 aagtgatgcc atcgtctgct ttggcggcct gggtggttgg cctggactga agtccgagcc
      481 ggacagcggt gtcaattatc ttgattattt ctgcaagaac tgcgtgggca aagtagacgg
      541 atttacagtg cattcttacg gaggtcccgc caaccaggct cttggtggct ctttggatga
      601 tccgaggaca tcagatattg ggagtttcgg tgcatataag gcattctgcg aagtcattta
      661 tgagaatttt cgaggatcca aacctgtcta tatcacggag agcaacacct actggtggat
      721 tgcgtcgcca catccccctc acagcatgga ttcctatcag gcaggatgga ttcagaaggc
      781 ttttcaggca atcgatcaat ggaatcaggc gagtgatctg aaggttcacg ccttgtgctg
      841 gtattcctat gcgcactatg gtgtgtgtga cggcaacgca ggcgatttct ggcacaacgc
      901 cattcagcca gggcgctgcc cgagtgatcc ggaactcgat caggcgcgtt ctgattatca
      961 atggacgacc accaacacgg actaccgctg ggacatcccc agcgacggta cactcaagat
     1021 tcaagtcgaa aactacaaca atagtgtgct ctggggagac accattgcca cgggatggga
     1081 aggcaccgac taccacgatt ccgaaccggc aaaccaaggc ggtggatacc gtcctgacgg
     1141 ggtggatatt gttgagaacg gtgacttgat ctatgtcggt tggacggaag cgggggaatg
     1201 gttacggtac actgtcgcag ggggaaagaa cacatggtat cgagtagacc ttcgctacgc
     1261 ccgacaagtg gcaggcaacg gacaatgtcg cattctggtg gatggagtga acgcaaccgg
     1321 aacgatcctc tgtccttcaa cgggtggatg ggagtcgttc agtacggcga gcagccccgc
     1381 gttcagcttg accgacggtg ttcatgacgt ccgattgctc ttcgatggca attaccttaa
     1441 tgcggactgg tttgccttcg taccagtcga agagccaacg gcgacgccaa cccgcacacc
     1501 gacaccgacg gtctctccca cgcggacacc atccccgacc tcaacacaaa ccaatacacc
     1561 aactccaact cgaacggcga caccaacgcc gatgccgacc gctccggctt cggcatccaa
     1621 ctggatgggg tactgaccgg cgggttagca agccgtgttc tgcgccacgc aagcattatc
     1681 tctgtattcc taagagaggc ataagtgttc ccaagaccag gaatgcaaac cacaaaacgc
     1741 tgaggccgat cgtagctcgt gcaaagttct tacgcatccg ttctttgcag aagcatgaat
     1801 acgtcgcgcc gatcagtgcc agactgaaca ccgcgaccag aaagagcagt ccaaggataa
     1861 gccattcatc aggctgagca cggcccatca gtcggacgta tcccgcatac tcagagacac
     1921 agtctatcgc tcttcccggg agactactct gatagtttgc agaccagata ctcagtccga
     1981 gatttgaata catctcttgc agccggggga aaaccagcag ggagtatagg gttggtatca
     2041 ccagaacgaa aactgccacg gtgcctacaa gcaagcgggt ctctcgcgtt cgctcaagag
     2101 cctttgcgga ttgctcgtca tcccaaggag taagactgca caggatgatg tacagcaaga
     2161 tcaaaagcgg cgacagcagg atggctgcgg caatgacgat accgcgaaca acgaccacat
     2221 ccacgttgag atactctgcc agtccggcgc agagtcctcc gacgacaccg tttgaccagc
     2281 tcttccggag tcttccccgc ttggcaacgg tctcttctgg ggtctctggc tgtttgggat
     2341 ttcgtgccat agcacgggat agtcgaaggt agtcctcttc cgagaccttt ccttcatgaa
     2401 acatctgatc gagcttgtcc tgtgatgtaa ctgcatttgc ttccattctc atagcctttc
     2461 tatcgcctcc gctgcttcgg acggcgttat ctcgcccttt tcaaggcggt caagaatcgc
     2521 ccttttggca tcctcatcgt ctcgcaattt ctgatatcgt tcgatcaacc tgtccaaacg
     2581 actccgaatt gcgacataac cgaaaccgga ctcctcggcg agcgccttaa tgctgaatcc
     2641 ggccagaaga tagcgctcga gaaactccga atccgcatcg gagagttgcg caaatcgtgt
     2701 ctgccgaaag cgacctcgga tctccacacc gcagtggtca catgccatgg tgacaggcct
     2761 cattggcgtc ttgcagtatg gacaatctgt ttgttttcta tctgacatca tggatgaaaa
     2821 atatctattt tatagaaaaa gtcaagtatt attttgatat tgtatctaat accacaataa
     2881 aattattgtc tggttgatgt gagtctgcgt ccttgtttgt ctgtaacccg agcaacgcat
     2941 gccgcaaatt gggaatttca ccaccaggaa atgtgggcac catgccacgt tgcgaggga
//
LOCUS       DAOQUC010000349         2995 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_38474_length_2995_cov_2.740136, whole genome shotgun sequence.
ACCESSION   DAOQUC010000349 DAOQUC010000000
VERSION     DAOQUC010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2995)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2995)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2995
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38474_length_2995_cov_2.740136"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2995)
                     /locus_tag="PKH07_07125"
     CDS             complement(<1..>2995)
                     /locus_tag="PKH07_07125"
                     /inference="COORDINATES: protein motif:HMM:NF020280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HNT34752.1"
                     /translation="FQVDIAVVKPDAIVVCELKDVGGPVVGSENGVWFVEDAQGSHPL
                     KGGRFPNPYLQARRLRASVLDLFKSRRAEFLNKRSQFVPFNRCVTAVVAFSPTEPDES
                     NIQIGSGSRWFSHCGVDKLHEVVAQQRAGIICLADDEIVRLIENTLSLQEARLIGTTP
                     MCPVSTSTAGEIQPEETPQVEPVTESVLIEDAELVPEPVVQTSEAEPEEIVLVPEKIT
                     VVRAPIVAAKRPSCIVCGLGAHACDVQHLKGRLLRINEKWCSLVISVDQIGEISVTYD
                     PRENPDLNETLPLMKSRLRADQRVTVAFWHLNRDENGLRMDEQSLFILEPDWLINVSD
                     LLNTDYCLRQVLVSRYGSDMPSESAILGNAVHHAFPETWEGCDQGKGMKAVQESLDSQ
                     ATDLVVTSTVPEDISDKAEEHIKNLCHFAQAKQKASILRRETFVLSPELGLKGKIDAI
                     WERDGQPVMVGELKTGKLSNYPKKNEAKEEHKLQVAAYAFMLIARGDADPRKLRTILL
                     YSIDLDNLCQAVVLDSGLLHRVMRTRNALVRLDFSGNAIFETNTRKCFPCSNRFTCRH
                     IGLLEAHADPRETSHVRKMLPAGDPFVSEDDRKLFSHYRGLITAEYAAVMRNVARLWG
                     MEPEQRALDGKALRLDSTRPPEGTKDPADGTYAYRLQTSHGEKNTSELRPNDRVLLSS
                     EDGLMAGRIATAKVTKVEADGIEVKMEEELLFCPAWADVQEDESLTERMYKGLYTFCS
                     SSHPLKKFLYHTNGDPTFTRECKDLHGPEWFEAVRSNARQTEALKRSLKADHFLLVNG
                     PPGSGKTALIARIVQAHLAEDKRVLVTAYTNRALDQVLGEIAEVVRESDWLRLGDPTS
                     SADEKMSAQTIEEIVRRDGLNTAQNMLLNRPVIGGTISSLLSGKFTEALGEFDLVIVD
                     EATQAKIPETLGVACLAERLVLIGDPQQLSPIVQKDSIPRDDSFSIGVSQNASSDLPD
                     LDVSLFEFLWERYGNVAGVALEQQYR"
BASE COUNT          660 a          843 c          765 g          727 t
ORIGIN      
        1 ccgatactgc tgttccagtg ccacgccggc gacgttgccg tatcgctccc aaagaaactc
       61 gaacaaggac acatccagat caggaagatc ggatgatgca ttctgcgaaa caccaataga
      121 aaaactgtca tctcgcggaa tcgaatcttt ctgaacaatg ggggacagct gctgtggatc
      181 tccaatgagc accaggcgtt ccgcgaggca ggcaacacca agggtttcgg gaatctttgc
      241 ctgcgttgcc tcgtccacga tgaccagatc aaattcgcca agcgcttctg tgaattttcc
      301 gctcaacagg ctgctgatcg tgccaccaat aaccggacgg ttcagtaaca tattctgtgc
      361 tgtgttcagt ccatcgcgcc gaacgatctc ttcaatggtc tgagcactca tcttctcgtc
      421 cgctgatgag gtcgggtctc caagtcgaag ccagtcgctc tcccgcacga cttcggcaat
      481 ctcaccgagc acctgatcca gagcgcgatt ggtgtaggcg gtgactaaca ccctcttgtc
      541 ttcggccaaa tgcgcctgaa cgattcgcgc aatgagagcc gtcttgccgg aacccggagg
      601 accgttgacc agaagaaagt gatccgcctt gagggagcgt ttaagcgcct cggtctggcg
      661 tgcattggaa cgcaccgcct caaaccattc cgggccgtgc aggtctttgc actcacgggt
      721 gaacgtcgga tcgccgtttg tgtgatagag gaatttcttc agcggatgcg atgagctgca
      781 gaacgtgtaa agccccttgt acatgcgttc ggttagtgat tcatcttcct gcacatcagc
      841 ccatgcgggg cagaacaaga gttcttcctc catcttcact tcgattccat cagcctccac
      901 tttggtaacc ttggcggtgg cgatgcgacc cgccatcagt ccgtcttcac tgctcaggag
      961 aacacgatcg ttgggacgca attcgctggt gtttttctct ccgtgagatg tttgcagtct
     1021 gtaggcatag gttccgtccg cagggtcttt tgttccttcg ggaggtctgg tggagtccag
     1081 tcgcaaagcc tttccgtcta atgcacgttg ttctggctcc atgccccaca agcgagcaac
     1141 attgcgcatc accgccgcgt actccgctgt gatgagtccc cgatagtggc tgaagagctt
     1201 ccgatcgtcc tcagagacaa acggatctcc cgcaggaagc atcttcctca catgggacgt
     1261 ctcgcgcgga tccgcatgtg cctcaagaag accgatatgg cgacaagtga atctgttgga
     1321 acagggaaaa cacttccttg tattggtttc aaaaatggca tttccgctga aatccaaacg
     1381 caccagagca tttcttgtac gcatcacgcg atgcaaaaga cctgaatcga gcacgacagc
     1441 ctggcaaaga ttgtccaaat caatgctgta cagaaggatg gttcggagct ttcttggatc
     1501 tgcgtctcca cgtgcgatga gcatgaatgc ataagccgcg acctgaagct tgtgttcttc
     1561 ctttgcctca ttcttctttg ggtaatttga cagcttgccg gtcttcagct ctccgaccat
     1621 aacaggttgc ccgtcgcgct cccagattgc gtctatcttt cctttgagac ccaattcggg
     1681 cgacagcaca aatgtctccc ttcgaagaat ggaggctttc tgtttcgctt gggcgaagtg
     1741 gcaaaggttc ttgatgtgct cctcggcttt gtcggagata tcttccggca cggtggatgt
     1801 cacgacgaga tcagtcgctt gagagtcgag cgattcctgc acggctttca tgcccttgcc
     1861 ctggtcacat ccttcccatg tttctggaaa ggcgtggtgt acggcgtttc caaggattgc
     1921 cgattcgctt ggcatatctg agccatagcg gctaacaagc acctgtctca aacagtaatc
     1981 ggtgttcaga aggtctgaaa cgttgatcaa ccagtccggt tccaggatga aaaggctctg
     2041 ttcatccatc cggagaccgt tttcatcgcg gttcaaatgc cagaacgcca cggtcacccg
     2101 ttgatccgcc ctgagccttg acttcatcag cggaagagtc tcgttcagat cgggattctc
     2161 gcgtggatca tacgttacgc tgatttctcc gatctgatcg actgatatga ccagagaaca
     2221 ccatttctca ttgatgcgca gaagccgtcc cttgagatgc tgaacatcac acgcatgagc
     2281 ccccaacccg cacacaatac aagacgggcg ttttgcagcc acgatgggag cccgaaccac
     2341 ggtgattttt tcaggaacca gaacgatctc ctcgggttcg gcttccgagg tctgaacgac
     2401 cggctccggc accaactcgg catcttctat caacactgat tccgtgactg gttcaacctg
     2461 aggggtttct tcgggctgta tttcgccggc tgttgatgtt gagacgggac acatcggagt
     2521 ggtgccgatc aatcgtgctt cctgcaacga aagtgtgttc tcgatgagcc ggactatctc
     2581 gtcatcagcg aggcagatga ttcctgctcg ctgttgtgcg acaacctcat gcagcttgtc
     2641 tactccgcaa tgtgagaacc agcgggaccc gcttccgatc tggatatttg attcatctgg
     2701 ctcggttgga ctgaatgcca caacagcggt cacgcaccgg ttgaatggca caaattggga
     2761 acgcttgttg agaaattccg ctcgtctgct cttgaacaga tcgagaaccg aagcacgcag
     2821 ccgccgcgcc tggagatagg gattcgggaa tcgcccccct ttcaggggat gactgccttg
     2881 ggcatcttcg acaaaccata ctccgttttc actgcccacg acaggtcccc caacatcctt
     2941 cagctcacaa acaacaatgg catcaggctt gactacagca atatccacct gaaag
//
LOCUS       DAOQUC010000350         2988 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_38605_length_2988_cov_3.309581, whole genome shotgun sequence.
ACCESSION   DAOQUC010000350 DAOQUC010000000
VERSION     DAOQUC010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2988)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2988)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2988
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38605_length_2988_cov_3.309581"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1378
                     /locus_tag="PKH07_07130"
     CDS             <1..1378
                     /locus_tag="PKH07_07130"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34753.1"
                     /translation="TLKKPSDELEDLSPSGCCKYRCEVRSDTVPFEELIAGDARLAEL
                     PRHWLNVFQYRPDIGILANNVVSDNVLFCMFTFSGLAPYTPLLPGNIEAIELVRESLD
                     RYFGGAPGYGQNTDHFTDSIPSMLIAAWDVIVTKGDMDLLSRWLPKMEQLAETIEGQD
                     RNGNGLLESRRTGNRGEGLEPHMRTGNWWDCINFGHEDAYVSALAYRAFRGLAHLERL
                     AGRVEQAARFDGDADRIKQVYFKTFYNSETGILAGWKSEDGELHDYWFTFINGIAIAY
                     DLVPEDEAGAIMDRLQAKMKEVGYDRFDLGLPGNLVPVAKNDYVFQGALGSPKEEDGS
                     DTFGVFENGGATACYAYFYVQALYQLGRREEADQILWPMIETYARGGFQNGVGKGGEW
                     RRWDGTPSGYEGFLADAYYSQLALFTGYYGILLGDVGFKLAPWSPLKGQKVATKLKFM
                     GKNVSWID"
     gene            1772..>2988
                     /locus_tag="PKH07_07135"
     CDS             1772..>2988
                     /locus_tag="PKH07_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019004995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNT34754.1"
                     /translation="MSEVEEKTPSDQGTYDEKSIVVLEGLEAVRKRPAMYIGSTSSMG
                     LHHLVYEVVDNSVDEAMAGFCDRIHVTIHFNNSVTVTDNGRGIPVGRHDKLKDKSAVE
                     VVLTKLHAGGKFDNKSYKTSGGLHGVGVSVVNALSEWLEIEVKRDGSVYYQKYARGIP
                     QSDLECVAKTKSTGTKVVFKPDPKIFEDTEFSFETLAGRLRELAFLNKGVEIVLEDER
                     SEKRSEFKYRGGIIEFVEYLSKGKQVLFPKPIALTRSKEIEREGKDPEFIELEIAIQY
                     NDSYNETVFSFANNINTKEGGSHLVGFRSALTRTINDYVRKNDLLKKSDLAITGDDVR
                     EGLCAVISVRISDPQFEGQTKTKLGNSEVKGLVESIVNEGLGEFFEENPSIARKVAAK
                     SISAAQAREAARKA"
BASE COUNT          744 a          724 c          850 g          670 t
ORIGIN      
        1 cacgctgaag aagccttccg acgaactgga agacttgagt ccgtccggtt gctgcaaata
       61 ccgttgtgag gtgcgttcag atacggtacc tttcgaggaa ctcatcgccg gagacgcccg
      121 acttgcagag ttgcccagac attggctcaa tgtgttccag tatcgtcccg acataggtat
      181 tctggcaaac aatgtcgtaa gcgacaatgt gctcttttgt atgttcacct tctcgggact
      241 ggctccttac acgcccctgt tgcccggcaa tatcgaggcg atcgaactgg ttcgggagtc
      301 actggatcgc tatttcggcg gcgctccagg ctatggtcag aacacagacc acttcaccga
      361 ttcgattccc tcgatgctca tcgctgcttg ggatgtcatc gtgaccaagg gtgatatgga
      421 tctgctttca cgttggcttc cgaagatgga gcagttggcg gaaaccatcg aaggacaaga
      481 ccgaaacgga aatggtctgc tcgagtccag gcgcacaggc aatcgcggcg aagggctgga
      541 gcctcacatg cgcacgggca attggtggga ctgcatcaac ttcggccatg aggacgccta
      601 tgtctccgct cttgcctatc gagcatttcg aggtctggcg catctcgagc gactggcggg
      661 tcgcgttgag caggcagcac gttttgatgg ggatgctgac cggatcaagc aagtctattt
      721 caagactttc tacaactccg agacaggaat tctggcgggc tggaagagcg aggatgggga
      781 actgcatgac tattggttca ccttcatcaa cggaatcgct atcgcctacg atttggtgcc
      841 cgaagacgaa gcgggcgcca tcatggatcg gctccaagcc aagatgaaag aggttggata
      901 tgaccgattt gatctggggc taccaggcaa tctcgtacct gtcgcaaaga atgactacgt
      961 cttccaggga gccttgggct caccgaaaga agaggacgga agtgacacat tcggtgtttt
     1021 tgagaatggc ggagcgacgg cctgttatgc ctatttctat gttcaggcac tctatcaact
     1081 cgggcgcaga gaggaagccg atcagattct ctggccgatg atagaaacat acgccagggg
     1141 cggattccag aacggtgttg gaaagggtgg cgagtggaga cgttgggacg gaacaccctc
     1201 aggctatgag ggctttctgg cggacgcata ctacagccaa ctcgctctct tcactggata
     1261 ctatggcatt ctgctcggtg acgtggggtt caaactggca ccttggtcgc ccctgaaagg
     1321 ccagaaagtg gcaacaaagt tgaagttcat gggcaagaac gtgagctgga tagactaggt
     1381 tccgaactgc ttaccccgga gccagatttt agcgcttgcc ggctgttgaa gtactctctc
     1441 tgttgctcag gacagaaatg tgcttgttca acaatttatt ggggtatctc cgctttctgg
     1501 tatcaagata ttgtcgtttg agggaagcgt tgtccttgac aaaagcagcc catttcccta
     1561 caatacaata gattttgcag attcagcagg ttgtgcctgc acccgtgtgg gcaatgccca
     1621 atttgtcggc agagtgctca tcgaggagaa cggagttctt tgggtctgct ctatgagagc
     1681 aggacttgta tttgtgtgca ttacgacgat gtgtcaagcc gacacacgcc agtttaagag
     1741 tgtttattct ttgggagagt gagaaacaac aatgtcggaa gtagaagaga aaacgccttc
     1801 agatcaaggg acttatgatg agaagagcat cgttgttctt gagggtttgg aagcggtccg
     1861 caaacgtcct gccatgtaca ttggcagcac cagcagcatg gggttgcacc acttggtcta
     1921 tgaagtggtt gacaacagcg tggatgaagc catggcgggc ttctgtgaca ggatccacgt
     1981 cacgatccat ttcaacaaca gtgttacggt tacggacaac ggccgtggga tccctgtcgg
     2041 gcgccacgac aagttgaagg acaagtccgc cgtagaagtc gtgctcacga aactgcatgc
     2101 gggtggcaaa ttcgacaaca agtcttacaa aacttccggc ggtctgcacg gtgtgggtgt
     2161 gtccgtcgtg aacgcactct ccgaatggct cgaaatcgag gtcaagcgcg atggcagtgt
     2221 ctactatcag aagtacgctc gcggcattcc acaaagtgat ctggagtgtg ttgccaaaac
     2281 aaagtcaaca ggcaccaaag tcgtcttcaa gccggatccc aagatttttg aagacacgga
     2341 gttttccttc gaaacactgg ccggacggtt acgcgaactg gcgttcctga acaagggcgt
     2401 ggagattgtc ctggaagacg aacgctccga aaagcgctcc gagtttaagt accgaggcgg
     2461 catcatcgag tttgttgagt acctcagcaa aggcaagcaa gttctatttc ccaaacccat
     2521 tgcgctcacc cgcagcaaag agatcgaacg cgaaggaaag gatccggaat tcatcgagct
     2581 ggagatcgcg attcagtaca atgacagcta caatgaaaca gtcttctcct ttgccaacaa
     2641 cattaacacc aaagagggtg gttcgcatct ggtcgggttt cgttcagcac tgactcgaac
     2701 gatcaacgac tacgtgagaa agaacgactt gctcaagaag agtgatctgg cgatcacagg
     2761 ggacgacgta cgagaaggct tgtgcgcggt catcagtgtt cgaatcagcg atccgcagtt
     2821 cgaaggacag acaaagacaa aactgggcaa cagtgaagtc aaaggcttgg ttgagtccat
     2881 tgtcaatgag ggactcggtg agttcttcga ggagaaccca agcattgcaa gaaaggtcgc
     2941 cgccaagtcc atctccgccg ctcaggctcg cgaagcagcg cgcaaagc
//
LOCUS       DAOQUC010000351         2988 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_38606_length_2988_cov_3.262871, whole genome shotgun sequence.
ACCESSION   DAOQUC010000351 DAOQUC010000000
VERSION     DAOQUC010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2988)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2988)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2988
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38606_length_2988_cov_3.262871"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1723)
                     /locus_tag="PKH07_07140"
     CDS             complement(<1..1723)
                     /locus_tag="PKH07_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012033277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HNT34755.1"
                     /translation="MRYDRLTLKSQEAIESAISNARERNHQEVDVEHLLIALIDQEEG
                     IVRPLLAKIGADVATLARDVEQALLQRPAVHGGGTSDVRMSKSLAQLLNAAEKEASRM
                     GDEYTSTEHLFLAIVSATASAVAAILKSHGLAKDKVLTALREIRGNQRVTSQSPEATF
                     NALQKYSRNLVELARRGDLDPVIGRDNEIRRVMQVLSRRTKNNPVLIGDPGVGKTAIA
                     EGLAQRVVSGDVPESLKNRQLLALDLGALVAGSKFRGEFEERLKAVLKEVENAKGDII
                     LFIDELHNLVGAGRAEGSMDASNMLKPALARGQLHCIGATTLDEYRKHIEKDAALERR
                     FQPVLVGEPSVEDSVAILRGLKERYEIHHGVRITDGAIVAAAKLSHRYITDRFLPDKA
                     IDLVDEAASRLRIEIDSMPTEMDEISRRIMQLEIEREALKKEKDPDSIKRRDAVQAEI
                     AELQEQLNQLKAHWQNEKDAIGRIQQLKERAEQLHQQEIEAQRSGNLERAAQIRYGEL
                     IENQKRLESEQQRLNELQKDTKMLKEEVEEEDIAEVVSNWTGIPVSRMLESEIEKLKQ
                     MEDRLRQR"
     gene            2059..2862
                     /locus_tag="PKH07_07145"
     CDS             2059..2862
                     /locus_tag="PKH07_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HNT34756.1"
                     /translation="MRLDQLLVERGLAKNREEAKRFILAGEILVEDRRDVKPGQMFAD
                     TVAIECRGKTCRYVSRGGEKLERALAVFNVNPSGLVCGDFGASTGGFTDCLLQHGACR
                     VYAFDVGQGQLAWSLRQDARVILTENCNLRFLQKEHVPEPLQIIAIDVSFISLSLILP
                     SASGVLAPEGAVICLVKPQFEIGKGRVGKGGVVRDPEDWKEVLNAHCQHAANSGFHIH
                     GLTVSPIQGSGGNVEFLSLLRETPCPDMNPSQAIQACIEAGSILKTAQS"
BASE COUNT          587 a          861 c          798 g          742 t
ORIGIN      
        1 ccctctgtcg aagccgatcc tccatctgct tgagcttctc gatctcgctc tccagcattc
       61 ggctgacggg aattcccgtc cagttggaga cgacttcggc gatgtcctct tcttcgacct
      121 cctctttgag catctttgtg tctttctgaa gttcgttgag gcgctgctgt tcggattcca
      181 accgcttctg attctcgata agctcgccat atcggatttg tgcggctctt tcgagattcc
      241 ccgaacgctg cgcctcgatc tcctgttggt gcaactgctc ggctcgttcc ttcaattgct
      301 ggatgcgacc gatggcgtcc ttctcattct gccaatgcgc ctttaactgg ttcagctgct
      361 cctgaagttc tgcgatttcg gcctgtaccg catcccgtcg cttgatggaa tcggggtctt
      421 tctccttctt gagcgcttca cgctcgatct ccaactgcat gatccttcgg gagatttcgt
      481 ccatctcagt cggcatactg tcaatttcga ttctcaagcg cgaagccgcc tcatccacga
      541 gatcgatggc tttgtcaggc agaaagcgat cggtgatgta ccgatgagag agtttggcgg
      601 ctgccacgat cgccccgtcg gtgatccgaa cgccatggtg gatttcgtag cgttccttca
      661 gtccccgaag aatggcgacg gagtcttcca cggatggctc accgacaagg acgggctgga
      721 aacgacgctc cagcgccgcg tccttctcaa tgtgctttcg gtactcgtcc aaggtggtcg
      781 caccgataca gtgcagctgc ccccgtgcaa gcgccggttt gagcatgttt gaggcatcca
      841 tggaaccttc cgcacgaccc gcgccgacga gattgtgcag ctcatcaatg aacagaataa
      901 tatcgccctt ggcgttctcc acttccttca agacggcctt cagtcgctct tcgaactcgc
      961 cacgaaactt gctgcccgcg accaaggctc ccaaatccaa ggcgagcaac tgacgattct
     1021 tgagagactc aggtacgtct ccggaaacaa ctcgctgcgc cagaccttcg gcaatcgccg
     1081 tctttccgac acccgggtct ccgatcagaa cggggttatt cttggtgcgc cgagagagca
     1141 cctgcatgac gcgccgaatc tcattgtcgc gtccgatgac cggatccaga tcccccctac
     1201 gggcaagttc gacgaggttt ctggaatact tctgcagtgc gttgaatgtt gcttcgggac
     1261 tctggctggt gacccgctgg tttcctctga tttcccgcag tgctgtcaga actttgtcct
     1321 ttgccaaacc gtgtgatttc agtattgcgg caacagcgct cgctgttgcg gagacgatgg
     1381 cgagaaacag atgctcggtg ctggtgtatt catcgcccat ccgagaggcc tctttctcgg
     1441 cggcgtttag aagctgggca aggctcttgg acattcggac atccgaagtc ccgcctccat
     1501 gaacggcagg cctctgcaag agcgcctgct ccacatcccg agccagcgtt gcaacgtcag
     1561 cgcctatctt ggccagcagt ggtctcacga ttccttcctc ctggtcaatg agcgcgatga
     1621 gcagatgttc gacatccact tcctgatgat tgcgctcgcg ggcattgctg attgccgatt
     1681 cgatggcttc ctgtgatttg agcgtcaatc ggtcataccg cattcttcat gcctcctcct
     1741 gggaaaccag actttgaaga agtctgctcc ctccaatgag caggcgtttg caggagcgcg
     1801 cacagcccac acgtctgcaa atgccagcct gctcagatgc cattgtcatc gggtgggtgg
     1861 ttcatccttc acatacaccg aaaaccgcgt gccacgtcag aaccttgcac ggtccaccac
     1921 ttcccgagaa ctcttcatca attgaacgat tcaacccccc aaagagtcta cgcttcgcgc
     1981 tgcaaagcgc atgttctttg tgacgtggtc tccttgcacg tgtctgtgta taatccacgc
     2041 atgagcagct gtcagaaaat gagactcgat caactccttg tcgagcgggg cttggcgaaa
     2101 aaccgagagg aagccaagcg atttattctc gctggagaga tcctcgttga agatcggcgc
     2161 gacgtgaaac ctgggcagat gtttgccgat acggttgcca tcgagtgtcg ggggaagacc
     2221 tgtcgctacg taagccgtgg tggggaaaaa cttgaacgtg ctctcgccgt cttcaacgtc
     2281 aatccttcag ggctggtgtg tggtgatttc ggagcgtcca caggcggctt taccgactgt
     2341 ctgctgcagc acggtgcctg cagggtctat gccttcgatg ttggacaagg ccaactggcg
     2401 tggagtctca gacaagacgc cagagtcatt ctgacagaaa actgcaacct tcgattcctg
     2461 caaaaggaac atgttccgga accgcttcag atcattgcca ttgatgtttc gttcatttcc
     2521 ctcagtctca tccttccgtc tgcttcaggt gtccttgctc ctgaaggagc ggtcatctgc
     2581 cttgtgaagc cgcagtttga aattggcaag gggagagtgg ggaagggagg cgttgtgcgt
     2641 gatcccgaag actggaaaga agttctgaat gcacactgcc agcacgctgc gaactcgggt
     2701 ttccacatac acggtctgac cgtctcaccg attcagggct ctggcggcaa tgttgagttt
     2761 ctcagccttc tcagggagac gccctgtcct gatatgaacc caagtcaggc gattcaggct
     2821 tgcattgagg cgggatcaat tctgaagact gcgcagtctt agggggtcga acgcgaccct
     2881 cgcaggggcg gcgtttttga tctcttggtc gcgcaaaggg attgaagaag ccagtccggg
     2941 tttcaagttc cggcgtcaaa tatctcttga caagtcctta gcgtgtca
//
LOCUS       DAOQUC010000352         2988 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_38611_length_2988_cov_2.456529, whole genome shotgun sequence.
ACCESSION   DAOQUC010000352 DAOQUC010000000
VERSION     DAOQUC010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2988)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2988)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2988
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38611_length_2988_cov_2.456529"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="PKH07_07150"
     CDS             <1..399
                     /locus_tag="PKH07_07150"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNT34757.1"
                     /translation="IRRQKKEFKMAKILLVDDDRDILEVLSLFLQAKGHEISASTNSQ
                     DGMKAVKSFQPDLVVLDVMMVQPDEGIVMAQQLRREGFSKPIILLTAISKVTGMTFGK
                     DDDLVPVDAFVDKPVNPDSLLTKINELLKK"
     gene            413..928
                     /gene="nuoE"
                     /locus_tag="PKH07_07155"
     CDS             413..928
                     /gene="nuoE"
                     /locus_tag="PKH07_07155"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005034123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HNT34758.1"
                     /translation="MLVTEQERLREQIAAWVDQYGSDRSTLMPVLQQVQKTYGRISNL
                     AMQEIADRLGIHPVEVYGVVSFYSFLHDKPQGRFVIRLCRTISCDMAHKDRVARQLEN
                     DLGVKFGETTSDGLFTLEWANCLGMCDQGPAMLINDEVHTRVTPESIHDILEKCRQTL
                     GASALTTEKES"
     gene            931..2121
                     /locus_tag="PKH07_07160"
     CDS             931..2121
                     /locus_tag="PKH07_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005034121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HNT34759.1"
                     /translation="MIKTKTGDLTFKSVALNKGAETALGMSRADIIGQLSESGIKGRG
                     GAGFPVSVKWNFAAAAQSDQKYVVCNADEGEPGTFKDRVILTDFADLVFEGMTIGAYL
                     IRATKGILYLRGEYTYLRGHLEAVLAKRRKEKLLGENAFGKKDFNFDIEIRLGSGAYV
                     CGEETALIESLEGQRGEPRNRPPFPVDTGFMGHPTIVNNVETLAWVAAIFANGASWFK
                     SVGTDRSRGFKLFSVSGDCERPGVYEFPLGITISQLLGTVGGEGAKAVQVGGASGNCV
                     PSCEFERTLAFEDVATGGSIIVFGQNRDMLQVAKNFMEFFLDESCGQCTPCREGNLKL
                     MEGLELLEQGRCPMKYLRELTALGETMQLASKCGLGQSSPNAFLTIVKHFKDEIMGRV
                     HLSH"
     gene            2250..>2988
                     /locus_tag="PKH07_07165"
     CDS             2250..>2988
                     /locus_tag="PKH07_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007039337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HNT34760.1"
                     /translation="MISVTIDDKELRVPMGTTILEAAKQVGIHIPTLCYHEDLCIAGV
                     CRVCVVEVEGQRTLQASCAFPITAPIKIKTHTAKVRKARRHILDLLLSRHYGECYACA
                     RNNNCELQRLAKEYGVDFFRFGHPDTPMHDVDRSSYSVVRDMNKCVLCRRCIRTCIDL
                     QEVGVLEVINRSNQSLVSTFMEKPLGEVICINCGQCINRCPTGALRANDPTDEVWAAI
                     DDPTKHVVIQTAPSPRAAIGECFGLEPG"
BASE COUNT          747 a          775 c          822 g          644 t
ORIGIN      
        1 atacgcagac agaaaaagga gttcaaaatg gcaaagattc tcttggtaga cgacgacagg
       61 gacattcttg aagtgctgtc cctctttctg caagccaaag gacatgagat cagcgcctcg
      121 acgaacagcc aggacgggat gaaggcggtc aagtccttcc agccagactt ggttgtgctg
      181 gatgtcatga tggttcaacc tgacgaagga attgtgatgg cgcagcagct gagacgtgaa
      241 ggcttcagca aacccatcat tctcctgact gccatcagca aggtaactgg tatgactttt
      301 ggcaaggatg atgatctcgt tcctgttgat gcctttgtgg acaagccagt gaaccccgat
      361 tccctgctca caaagatcaa cgaactactc aagaaatagg agggcgtttc cagtgctcgt
      421 aacagaacag gaaagattgc gtgaacagat agcagcatgg gtggaccaat acggcagtga
      481 cagaagcacc ctgatgccgg ttctgcaaca ggtacagaag acatacggca ggatttcgaa
      541 cctcgcgatg caggagattg cggacaggct cggcattcac ccggttgaag tctatggcgt
      601 cgtatccttc tacagttttc tccacgacaa gcctcagggg cgctttgtca ttcgactttg
      661 ccgcacgatc tcatgcgaca tggcacacaa ggatcgcgtc gcccgccaat tggaaaacga
      721 tctgggagtg aaattcggtg agacaacatc ggatgggctg ttcacactcg aatgggcgaa
      781 ttgcctggga atgtgcgacc agggcccagc aatgctcatc aatgacgagg tccacacaag
      841 agtaacccct gagagcatcc acgacatcct tgaaaagtgc cgtcagacgc tgggtgcctc
      901 agctctcacc accgagaagg agtcctgaac atgatcaaga caaagacagg cgatttgacc
      961 ttcaaatccg tagcattgaa caaaggcgca gaaactgctt tggggatgag ccgtgccgat
     1021 atcatcggcc agctgagcga gtccggcata aagggacgag gcggagcagg tttcccagtc
     1081 agtgtgaaat ggaattttgc cgccgcggca cagagtgatc agaagtatgt cgtctgcaat
     1141 gccgatgaag gggaaccagg taccttcaaa gaccgcgtca ttctgacgga tttcgccgat
     1201 ctggtctttg agggaatgac cataggggca tacctgatcc gagccaccaa gggaattctc
     1261 tatcttcgcg gcgaatacac ctatctgcgt ggacatcttg aggcagtgct cgccaagaga
     1321 cgcaaagaga aactgctggg tgaaaatgct tttggcaaga aggatttcaa ctttgatatt
     1381 gagatccgtc tcggttcggg tgcctatgtg tgcggagagg aaacagccct catcgaatcc
     1441 ctggaaggac agcgtggcga gcccagaaac cgcccacctt tccccgtaga caccggattc
     1501 atgggacatc caaccatcgt caacaatgtc gagacgctgg cttgggttgc cgccattttt
     1561 gccaatggtg cctcttggtt caagagcgtt gggacagacc gctcaagagg tttcaaactc
     1621 ttcagtgtct ccggagactg cgaacgccct ggggtctacg agttcccact gggaataacg
     1681 atcagccagc tgcttggtac cgttggcggc gaaggcgcca aagcggttca ggtcggagga
     1741 gcatcgggca actgcgttcc cagttgcgaa ttcgaaagga ctctcgcttt tgaagatgtc
     1801 gccactggcg gatcgattat cgtcttcgga cagaatcggg acatgcttca agttgccaag
     1861 aacttcatgg agttcttcct ggatgaatcc tgtggtcagt gcactccgtg tcgagagggt
     1921 aatctcaaac tgatggaagg tttggaactg ctcgagcagg gacgttgccc gatgaagtat
     1981 cttcgtgaac tgaccgctct tggcgaaacg atgcagttgg catccaagtg cggcttggga
     2041 cagtccagtc ctaacgcctt tttgacgatt gtcaaacact tcaaagacga gatcatggga
     2101 cgcgttcact tgtcccattg aacgatgaaa ggtgtacaac catgtcgaat aagaagactt
     2161 ccgtctcctc agatgcaatg tctcgcgctc ccaaaagtca gcgagtaggt ccggtcgcag
     2221 cctccgccga ttccaaagac attggcgcga tgatctctgt caccattgac gacaaggaac
     2281 ttagggttcc aatgggaacc acaatccttg aagcggctaa gcaggttgga atccatatcc
     2341 caacactctg ctatcacgaa gatctgtgta tcgctggagt gtgccgcgtg tgcgtggtcg
     2401 aagtggaagg gcagcgcacg cttcaggcgt cctgcgcctt tccgatcacc gcgccgatca
     2461 agatcaagac tcatacggcc aaggttcgaa aggccagacg ccatattctg gatctgcttc
     2521 tctccagaca ctacggagaa tgctatgcct gcgcccgcaa caacaattgc gagcttcaaa
     2581 ggcttgccaa ggagtatggc gtagacttct tccggttcgg ccatcccgac acgccgatgc
     2641 acgatgtgga tcgctccagt tactcggtcg ttcgggacat gaacaaatgc gtgctgtgcc
     2701 gccgctgtat tcgcacctgc attgaccttc aggaagtggg cgttctggaa gtcatcaacc
     2761 ggagcaacca gtccttggta tccacattca tggaaaagcc tctcggtgag gtcatctgca
     2821 tcaactgcgg ccaatgcatc aaccgatgtc cgaccggagc gctcagagcc aatgatccaa
     2881 ccgatgaagt ctgggcggcc attgatgatc cgacaaagca tgtggtcatc caaaccgcac
     2941 ccagtccgcg agcggccatc ggagagtgtt ttggacttga accgggca
//
LOCUS       DAOQUC010000353         2984 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_38677_length_2984_cov_3.351314, whole genome shotgun sequence.
ACCESSION   DAOQUC010000353 DAOQUC010000000
VERSION     DAOQUC010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2984)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2984)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2984
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38677_length_2984_cov_3.351314"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="PKH07_07170"
     CDS             <1..324
                     /locus_tag="PKH07_07170"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HNT34761.1"
                     /translation="DCGVVSMETAVMTTEDVLNRLRDFKNKRQTEFGLVSLGVFGSFA
                     RGQAGAESDVDVFFETTEPNLFRTARMKRELEQVMSRRVDVVRLREQMNPRLRRRILQ
                     EACYV"
     gene            317..694
                     /locus_tag="PKH07_07175"
     CDS             317..694
                     /locus_tag="PKH07_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006509550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HNT34762.1"
                     /translation="MFDRPEILDRLSDLLQALERIPKRCSSINSPEDFLVDDMGQEHL
                     DSICMVLVAAGEAFRWLDAKTERKFLSRYQQIPWRDVIAVRNVIAHGYFDVDTEQVFS
                     ICKKDIPELIEVVRMMIRDLQAD"
     gene            complement(729..1253)
                     /locus_tag="PKH07_07180"
     CDS             complement(729..1253)
                     /locus_tag="PKH07_07180"
                     /inference="COORDINATES: protein motif:HMM:NF024135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HNT34763.1"
                     /translation="MVEILVTYATKYGATREIAERIGETLRQLGLEIEVESVEHVGDV
                     SRYKAIVLGSGVYAGRWRKEAARFLVTNQDELARHLVWLFSSGPTGKGDPVELMKGWR
                     FPAGLQTVADRIRPRDIALFHGVLNVSKMNIIERLLVKMIKAPLGDFRDWNAISAWAD
                     KIAEGLEHPTPRSF"
     gene            1363..2634
                     /locus_tag="PKH07_07185"
     CDS             1363..2634
                     /locus_tag="PKH07_07185"
                     /inference="COORDINATES: protein motif:HMM:NF018743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HNT34764.1"
                     /translation="MLHFVHVLIILCLLFASGMYLAWPVAAQMDSSAFQQQLQSLMNT
                     LHGKRVALLTNPTGVDDHFDFIADRLISDASITLVCFFAPEHGLRGDQQAGGGVQDYT
                     DPISGLPVYSLYGSRLAPTADQLQGVDVLVFDIQDVGVRFYTYVWTMTYAMESAAANG
                     VKFVVFDRPNPIGCDRVEGAPIPFDGGLVGRLWPGQPFGVSTRHGMTVGEIARLVNGA
                     WMNPKAELEVIPIPGYTRSMTFQQTGYPWVIPSPNMPTIDTATVYPGTCVFEGVNLSE
                     GRGTTRPFEFIGAPFINAFDYANALNALDLPGVRFRAAYFTPMFDDHAGKQCGGVQVH
                     VTNVNQFDPIRTGLYMLKTVVTMYPSNVTVGSYVSTLMGFSNLHTRIQTEQVDSLIAE
                     WQTNLDAFKTLREEYIIYPTSVGGVSLWATQ"
     gene            2652..>2984
                     /locus_tag="PKH07_07190"
     CDS             2652..>2984
                     /locus_tag="PKH07_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015720790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNT34765.1"
                     /translation="MSTTEESVSALAIQTRNLCVQYRRFLRPAVDALDGLNLSVSYGE
                     VFGFLGPNGAGKSTAIKTLLGLVFARTGSAQILGRPAGSLEARRRIGYLPEVALYYPY
                     LKASEMLAM"
BASE COUNT          647 a          800 c          794 g          743 t
ORIGIN      
        1 gattgtggag tagtctcaat ggaaactgca gtgatgacaa cagaggacgt tctcaaccgg
       61 ctgagggact tcaagaacaa acgtcagact gagtttggtt tggtctccct tggcgtcttt
      121 ggctcttttg cccgtggcca ggctggcgca gagagtgacg tggatgtctt tttcgaaacc
      181 actgaaccga atctcttccg cactgcgagg atgaaacgcg agttggagca agtaatgtcc
      241 cgtcgcgtgg atgttgtcag gttgcgagag cagatgaacc ctcgcctgag acgccgcatt
      301 ctccaagagg cttgctatgt ttgaccgacc agagattctc gacagactta gcgatctgct
      361 ccaagccttg gagcggattc caaagcgctg ctcttccatc aattcaccgg aggatttcct
      421 ggtggatgat atgggacaag agcatcttga cagtatctgc atggttcttg tcgcggcagg
      481 agaggccttt cgatggcttg acgcaaagac ggagcggaaa ttcctatccc gctatcaaca
      541 gatcccctgg cgggatgtga ttgctgtgcg caatgtcatc gcccatggat acttcgatgt
      601 tgacaccgag caagtgttca gtatctgcaa aaaggacatt cctgaactca tcgaagtggt
      661 tcgcatgatg atcagggatc tgcaggcaga ttgagtgtca ccttgcctga atctgtagct
      721 ctgtgcgatc agaaagaccg tggcgttgga tgttctaacc cctcggctat cttatctgcc
      781 caggcggaaa tggcattcca gtctcggaaa tcaccgagtg gtgccttgat catcttaacg
      841 agcagccttt caatgatatt catcttgctc acgttcaaca cgccatgaaa cagagcgata
      901 tcacggggac gtatgcggtc tgcaacagtc tgcagacctg ctgggaaccg ccaacctttc
      961 atcaactcca cgggatcccc tttgcctgtt ggcccgctcg agaaaagcca gacgagatgt
     1021 ctggccagct catcttgatt ggtgacaaga aaccgcgctg cttccttgcg ccatcgcccg
     1081 gcgtacactc cactgcccag gacgattgcc ttgtagcgag aaacgtctcc cacatgctcg
     1141 acagactcca cctcaatctc caatcccagc tgccgtagcg tctcaccgat cctctcggca
     1201 atctcgcgtg ttgctccgta cttggtcgca taggttacaa gaatctcaac catgggatgc
     1261 tcctcttttg taggatatgg gccaaccaat tcgcaccact tgcactcttt tctgaatacc
     1321 aataccatca tacattaaag aacttcagga ggaaattgtc acatgcttca tttcgtgcac
     1381 gttctgatta tcctgtgtct cctttttgca tcaggaatgt accttgcctg gcctgttgct
     1441 gcacaaatgg attcgagcgc tttccaacag caactgcagt cgctgatgaa cacccttcac
     1501 ggcaagcgag ttgccctctt gacgaatccg acgggtgtgg acgatcactt cgacttcatc
     1561 gccgaccgac tgatctccga tgccagcatc accttggtct gcttctttgc gccggagcat
     1621 ggacttcgcg gagatcaaca ggcgggaggc ggcgttcaag actacacgga tcccataagc
     1681 ggtctgccgg tctattcgct ctatggttca cgtcttgcgc cgaccgctga tcaacttcag
     1741 ggcgtggatg tgttggtgtt tgacattcag gatgttggcg ttcgtttcta cacgtatgtc
     1801 tggacgatga cctacgcgat ggaatccgcg gcggccaatg gtgtgaagtt tgtggtcttt
     1861 gatcggccaa atcccattgg atgcgatcgg gtcgaaggtg ctcccattcc atttgatggg
     1921 ggtctggtcg gacggctctg gcctggacag ccttttggcg tctcgacgag gcatgggatg
     1981 accgtgggtg agatcgctcg tctggtcaat ggtgcctgga tgaaccccaa agccgaattg
     2041 gaagtcattc caatccccgg ttacacgcgc tcgatgacat ttcagcagac gggctatccc
     2101 tgggtgattc cttctccaaa catgcccacc attgacacgg caacagtcta tcctggtact
     2161 tgtgtctttg agggagtgaa tctcagcgaa ggcagaggaa caacccgacc ttttgagttt
     2221 atcggagcgc cattcatcaa cgcgttcgac tacgccaatg cgctcaatgc gctcgatttg
     2281 cctggtgtgc gttttcgagc cgcctacttc actcccatgt ttgacgacca cgctgggaag
     2341 cagtgcggtg gcgttcaggt gcatgtcacc aatgtcaatc agttcgaccc tatcagaaca
     2401 ggcctctata tgctgaagac tgtagtgacg atgtatccca gcaatgtcac cgtcggctct
     2461 tacgtctcca ctctgatggg tttttcgaat ctccacacgc gaatccaaac agagcaagtg
     2521 gattccttga tcgccgaatg gcagacgaat ctggacgcct ttaagacgct tcgggaagag
     2581 tacattatct atccgacgag tgtcggtggc gtgagtctct gggcaacgca gtgacttggg
     2641 agtgtcagag catgagcacg acggaagaga gcgtttcggc attggcaata cagactcgga
     2701 atctctgcgt tcagtaccga cgattcctgc gacctgccgt tgatgccctg gatggtctga
     2761 acctttccgt gtcgtacgga gaggtgtttg gctttctggg acccaacgga gcaggcaaga
     2821 gcactgcgat caaaaccttg ctgggtcttg tttttgcgcg aacgggctct gcgcagattc
     2881 tcggcaggcc ggctggctcc ctggaagcca gaagacgaat cggatacctt ccagaggttg
     2941 cgctctacta tccctatctg aaagccagtg agatgctcgc catg
//
LOCUS       DAOQUC010000354         2980 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_38749_length_2980_cov_3.462564, whole genome shotgun sequence.
ACCESSION   DAOQUC010000354 DAOQUC010000000
VERSION     DAOQUC010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2980)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2980)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2980
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38749_length_2980_cov_3.462564"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1635
                     /locus_tag="PKH07_07195"
     CDS             <1..1635
                     /locus_tag="PKH07_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34766.1"
                     /translation="YASILSSKNRFYTHFNSHFVEFDPRTRAFTFFQKTTPQMAMAMT
                     EDDNGVIWSVTYPDSGVVSFDPSAVALRDYGSLNKENWRQYPRHIAADDKGWIYFGIG
                     STASQIIILDPKTGKSQTVLKDDERLKGYATVYRDVNGKVYGNSGGGSKEDGYEFYVG
                     EAKKVLTPENINKKPFISSSQALFHGEFPDGRKLRRCDLVERLIAVENPETGEVKEST
                     FEYTSEGAHLMGLATAPDGTICGGTAFPMRFFSYNPKTDEWINRAAYGQWNTVAAKED
                     RFFVGGYTGGFLLEWDPSKPWVPTVKGEDGCNPKYLTECKPTINRPHYLLAHPDGKTL
                     VLAGTPDYGYTGGGLLFWDRETQTQTVIEHTEILAEHSTASMVALPEGKLLGGSTTSP
                     GTGGETKAKEAELYIMDMVSKKVEWHSAVFPGAQYYTDMVGMATGLVYGVADRKIFFV
                     FDPVKRVVVHQSDVTAEFGLSIYHQGPRVFVPAADGSLYMLFVKGVAQVDLNTYAITL
                     LAESPVPIHAGGDILDGRIYFSSGSHLYSYEVPAAR"
     gene            1781..2764
                     /locus_tag="PKH07_07200"
     CDS             1781..2764
                     /locus_tag="PKH07_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNT34767.1"
                     /translation="MSQDAVGIETCNLEKSFDDIRAVDGITFSVDRGELFGFLGPNGA
                     GKTTTINILTGLARPDSGTVCIAGINCTHNAKAAQHLIGVVPDESNLYPELTGYENLC
                     FCAALYGMRKRERQERARQILGVFGLDQAANRLFGGYSKGMKRKLTIAAGIIHSPEIL
                     FLDEPTSGIDVASARQIRRIIADLNHAGTTVFLTTHYIEEAERLCNRIAFVVRGRIVA
                     NDSVENLLQPVMERHIVRISISSVPQDLHQSLCSAFPELEFTLEERFQIRVESDREIR
                     VGSLVRFLEEQGAEVSEARRLRPSLEDVFVRVTGIEGDAMRKEKEKKGGSG"
     gene            2761..>2980
                     /locus_tag="PKH07_07205"
     CDS             2761..>2980
                     /locus_tag="PKH07_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNT34768.1"
                     /translation="MKTWIAFWNILLKDIRTYYLKPPNISWGLVFPLAWAGMFFIKSG
                     TGLESIPRVLPGLVAVSVLFGTTSMLAVT"
BASE COUNT          743 a          760 c          818 g          659 t
ORIGIN      
        1 tacgcttcga tcctgtcgag caagaacaga ttctacactc atttcaacag tcactttgtc
       61 gagttcgatc cacgaacgcg tgcgtttaca ttcttccaga agaccacccc acagatggcg
      121 atggcaatga cggaagacga taacggcgtc atctggtcag taacctatcc agacagcgga
      181 gtcgtgtcgt ttgatccttc cgcagtcgct ctgagagact acggttccct caacaaggaa
      241 aactggagac agtacccacg tcacatcgca gcagacgata agggttggat ctacttcggc
      301 atcggcagca cggccagcca gatcatcata ctcgacccca agaccggaaa gagtcagacc
      361 gttctcaagg acgatgaacg tctgaagggc tatgcaacag tctaccgtga cgtcaacggc
      421 aaagtgtacg ggaactcagg tggaggctcg aaggaagacg gatacgaatt ctacgttggc
      481 gaagccaaga aggtgctcac accagagaac ataaacaaga agccatttat cagctccagt
      541 caagccttgt tccatggaga gttccccgac ggcagaaaac ttaggcgctg cgatctggtc
      601 gaacggctga tcgccgtcga gaatccggag acgggcgagg tcaaggaaag tactttcgaa
      661 tacacgagcg agggcgcaca tctcatgggg cttgccacgg ccccagacgg cacgatctgc
      721 ggcggcacag cgttcccaat gcgtttcttc agctacaatc cgaagacaga tgaatggatc
      781 aaccgtgctg cctatggtca atggaacact gttgctgcaa aagaagatcg cttttttgtt
      841 ggcgggtata cgggaggatt cctgctggaa tgggatccgt ccaagccatg ggttccaacg
      901 gtgaagggcg aagatgggtg caaccccaag tatctcactg aatgcaagcc aaccatcaac
      961 cgtccgcact atctcttggc gcaccccgac ggcaagacat tggttcttgc gggaacaccg
     1021 gattacggat acacgggtgg cgggctgttg ttctgggatc gggagaccca aacccagaca
     1081 gtgatcgagc acactgagat tcttgccgag cattccactg caagcatggt ggctcttcct
     1141 gaaggcaaac tgctgggcgg aagtacaaca agccctggaa ccggaggcga gacgaaagcc
     1201 aaggaagcgg aactttatat tatggatatg gtttcaaaga aggtcgaatg gcatagtgcc
     1261 gtcttccccg gagcacagta ctacacggat atggtcggca tggcaacggg tcttgtctac
     1321 ggggttgcgg acaggaagat tttcttcgtc ttcgatcctg tgaagagagt cgttgttcat
     1381 caatcagacg tgacagcgga gttcggactt agcatctacc atcaaggacc tcgtgttttt
     1441 gtgcctgcag ctgatggatc gctctatatg ctctttgtca aaggagtcgc gcaggtggat
     1501 ttgaacacgt atgcaatcac gctgctcgcc gagtcacccg tacctatcca tgcagggggt
     1561 gacatcctcg acggacggat ctacttctcc agcggatcgc atctgtacag ctatgaggtg
     1621 cctgctgcac ggtgagatac aagtgtgaga ccgcttaggc tcgccctcta cagcctaaga
     1681 tctatgcgct ctcaacactg caggatcact atgagatagt acgcgactca gacaggctcg
     1741 tgacagtctg attgaggcga tgaacaaagg attcggaaaa gtgtctcaag atgctgttgg
     1801 catcgaaaca tgcaatctgg aaaagagttt cgacgacatt cgagccgttg atggcatcac
     1861 attctctgtt gatcgagggg agctgttcgg ttttctcggc cccaacggtg caggaaaaac
     1921 cacgacaatt aacatcctga caggcttggc gcgcccggat tctggaaccg tctgcatcgc
     1981 gggaatcaac tgcacgcaca acgccaaagc ggcacagcat ctcatcggag tcgttcccga
     2041 cgagagtaat ctctatccgg aactcaccgg atatgagaac ctgtgtttct gcgcagctct
     2101 gtatggaatg cgcaagcgcg agcgccaaga gcgtgcgcga cagattctag gtgtctttgg
     2161 attggatcag gcggcgaatc gtctcttcgg tggctactca aaaggaatga agagaaagct
     2221 caccatcgct gccggaatca tccacagtcc ggagattctc tttcttgatg aacccacctc
     2281 tggcatagat gtagcgagtg cacgacagat caggcggatc atcgctgatc tgaaccacgc
     2341 tggcacaacc gtattcctga ccacccacta catcgaagag gctgagcggt tgtgcaatcg
     2401 tatcgctttt gtcgtccgcg gtaggattgt ggcgaacgac agcgtggaga atctcctaca
     2461 gccagtcatg gaaaggcata ttgtacggat ttcgatttcc agcgtacctc aggacttaca
     2521 ccagagcctt tgctcggcat ttcccgagct tgaattcaca cttgaggaac gtttccagat
     2581 tcgtgtggaa tcagaccgag agattcgtgt cggatccctt gtgcgttttc ttgaagagca
     2641 aggtgccgaa gtatcggagg ctcgaagact gcgtccatcg ctcgaggatg tatttgtgcg
     2701 tgtcaccggt atcgaagggg acgcaatgcg aaaggagaag gaaaagaagg gaggcagcgg
     2761 gtgaagactt ggatcgcctt ttggaacatt ctgcttaaag acattcgaac ttactatctc
     2821 aagccaccca atatcagttg gggacttgtc ttcccacttg catgggcggg catgtttttc
     2881 atcaaatccg gcactggcct ggaaagcatt ccacgagtgc ttcccggttt ggtggccgtg
     2941 tctgttctct ttggcacaac gtccatgctg gcagtaacag
//
LOCUS       DAOQUC010000355         2972 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_38895_length_2972_cov_3.751800, whole genome shotgun sequence.
ACCESSION   DAOQUC010000355 DAOQUC010000000
VERSION     DAOQUC010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2972
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38895_length_2972_cov_3.751800"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1806
                     /locus_tag="PKH07_07210"
     CDS             <1..1806
                     /locus_tag="PKH07_07210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34769.1"
                     /translation="VRCLPDLPGSLAVVLLLLALEMNARPLVLGLVFAIVCCLKPTFA
                     TLLPLVLWGACDEKSWKARLTAALYVGFGSLLIIWPWMLRNFLITRNPFYSFLGYDLL
                     SHTPSFRNVYVWNLTDAPNPMGFALRHPIEMLLKVFQVIRFTAGFCRPEAMWVLFFAI
                     PAMLFFLPRIAMRNRLLAAIMLIVLPLFVTHTEARYFAPVLVPLVVLCLWVTEHKLRE
                     RTGAWPLAVFWTASVLVAFLLLNVSGLAGLRKHSYSRNWLRWDQVAFFENRTTPEHLI
                     ASDVPFAISWECNRTTTFLPFREGDLRAVDKVHPVDFVFVQQPRFNASLHDYEKSTWF
                     QENFRILNIFDDDTGSRLYRRRTSILGGFDAPQLCQVRGNLFVLRERIVHSVDLPSLS
                     RHPIQTVPEGVVRINGDETTGGFHLMSDDGTILGLAGAAGYNPEPPLEGVPIDFKFTP
                     NGQGIFFLMDDGRVVPRGDAVDFGVRAGVIEQPLRLAVTSTGKGVLVLSADGTVVVCG
                     DAKSGYERYFGEPLARDIALVPGTNDYYILDAFGSTHPSRTDSILAVGPYRADRRWAL
                     DLEILPGGAPYVLSDEGEILRYELKRADQVQATVP"
     gene            1847..>2972
                     /locus_tag="PKH07_07215"
     CDS             1847..>2972
                     /locus_tag="PKH07_07215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34770.1"
                     /translation="MIRKWISLIFSFAAVCSIAPCGGSALEVVNAQYHADVHLPQYLP
                     LYHEGWNQPDKDGQPIQYAHDEMQLGGYLHVFFQNVSDKTVQVDDVLLGSVSEGVHSS
                     EGLSSARGVSPKEGIRWGGNARSGIRDAHLCFSKLPKSDLDKLHELGEPVWWKVNPVF
                     IEPGRIAEVVIRLRRNPKVSNVAVTVLAGVMTFEATVDTRQETPRLQGLSFSQNLDTV
                     FLYARHPKGKGMIPVQVIFDGQVLTDRCVVGNDPSMEVIPICLSLERPLEKGSVHYYQ
                     VTYPDSLSAAAVQRAWSDQLAYGMWGSRKKGQTSDEQVREYLTNMSRHNINVHMGMGD
                     PRPDELMLTEDGIAFCQSIGIRGMSLNFGNMKNPFFYFLMD"
BASE COUNT          598 a          755 c          828 g          791 t
ORIGIN      
        1 gtccgatgcc ttcccgatct cccaggatct cttgctgttg tgctgctgct cctggctctg
       61 gagatgaatg cgagacctct cgtcttgggt ttggtttttg ccatcgtctg ttgtctgaaa
      121 ccgactttcg ccactctgct gcctctggtc ttgtggggag cctgcgacga gaagtcgtgg
      181 aaagcacgcc tcaccgcagc tctgtatgtt ggctttggtt cgcttctgat tatctggcca
      241 tggatgcttc ggaatttcct tatcacgcga aatccgttct acagttttct gggctatgat
      301 ctgctgagtc acacgccatc ttttcggaat gtctatgtct ggaatctgac ggatgcaccc
      361 aatcccatgg gatttgcatt aaggcatccg atcgagatgc tgctcaaggt gtttcaggtc
      421 atccgattta cggcgggatt ctgccgtcct gaagcaatgt gggtattgtt ctttgccatt
      481 cccgcgatgc ttttctttct gcctcgaatc gcgatgcgca accgtttgct ggctgccata
      541 atgctcatcg ttttgcctct ctttgtcacg cacacggaag cgcgctactt cgcgcccgtg
      601 ctggttccac ttgtggtgct gtgcctttgg gtcacggagc acaaactgcg agaaagaacc
      661 ggtgcgtggc ccttggccgt gttctggaca gcatcggttc tcgttgcctt tctcctgctg
      721 aatgtcagcg gccttgcagg actgagaaag cacagttaca gtcgcaactg gcttcgctgg
      781 gatcaggttg ccttctttga gaaccggacg acaccagagc acctgatcgc cagtgatgtt
      841 cccttcgcca tcagttggga atgcaaccgc acaacgacgt ttctgccgtt tcgagaagga
      901 gatctgcggg cggtggacaa agtccatcca gtggatttcg ttttcgtcca gcaaccacgt
      961 ttcaacgctt cgcttcatga ctatgagaag agcacatggt ttcaggagaa cttccgaatt
     1021 ctcaatatct ttgacgatga cacgggctcc aggctctacc gacgacggac aagtatcctt
     1081 ggtggctttg acgctcccca gttgtgtcag gttcgcggca acttgttcgt tcttcgtgag
     1141 agaattgtgc attcggttga tctgccatcg ctgtcccgtc atccaattca gactgttcct
     1201 gagggtgtgg ttcgcatcaa cggcgacgag acaaccggtg gattccattt gatgagcgac
     1261 gatggaacga ttctggggct ggcaggtgcg gctggctaca atccggagcc cccgctcgaa
     1321 ggcgttccca ttgacttcaa gttcactccg aacggtcaag gcatcttctt cctcatggat
     1381 gatggtcggg ttgttccgag gggagacgcg gttgactttg gagtccgggc tggagttatc
     1441 gaacagcctc ttcggcttgc cgtgacctca acagggaagg gagtccttgt actgtctgcc
     1501 gacggaacgg ttgttgtctg cggtgatgcg aaatctggat atgaaagata tttcggagaa
     1561 cccctcgcgc gggatattgc gctggttcca ggaacgaacg actattacat tctcgacgcc
     1621 tttggctcta cacacccgag caggacagat tcgattctgg cggtaggacc ctatcgtgcc
     1681 gaccgacgat gggcgttgga tttggagatt ctacctggcg gcgctccata cgttctttca
     1741 gacgaaggcg agattctgcg ctatgaattg aagcgtgccg accaagttca ggccacagtc
     1801 ccttgagtga aaccgaggaa ctatcttgcc ccttagggag gtttcgatga tcagaaaatg
     1861 gatttctcta atcttctctt tcgcggccgt ctgttctatc gcgccttgtg ggggatcggc
     1921 gcttgaggtc gtcaatgcgc agtaccatgc cgatgtccac cttccacagt atctgcctct
     1981 ttaccatgag ggttggaatc agcctgataa agacggtcag ccaattcagt atgctcacga
     2041 tgagatgcaa ctgggggggt atctgcatgt gttcttccag aatgtcagcg acaagactgt
     2101 tcaggttgat gatgtccttc ttggctctgt ttctgagggc gtacactcat cagaaggctt
     2161 gagttcggca aggggtgtca gtcccaagga aggcatccgc tggggtggca acgcccgttc
     2221 tggaattcgt gatgcgcatt tgtgtttctc caaactgcct aagtctgatc tggacaaact
     2281 ccatgaactt ggtgagcctg tctggtggaa agtcaatcca gttttcatcg aacctgggag
     2341 aatcgcggaa gttgtcattc ggcttcgaag gaacccgaaa gtctctaatg ttgcggtgac
     2401 ggtattggct ggcgtgatga cctttgaggc aacggttgac acaagacaag agacgccgag
     2461 gcttcagggg ctgagtttct cccagaatct ggacacggtc tttctctatg ctcgtcaccc
     2521 caaggggaag gggatgatac ccgtccaggt gatctttgat gggcaggtct tgaccgatcg
     2581 ctgtgttgtt gggaatgatc cttcgatgga agtgattcct atctgccttt cactcgaaag
     2641 gccgctcgag aagggttctg tccattacta tcaggtcacc tatccagaca gtctgtcagc
     2701 agcggcagtg caacgggcat ggagcgatca actggcctat ggcatgtggg gctcccgcaa
     2761 aaaagggcag accagtgacg agcaggtgcg cgagtacctg acgaatatgt ccaggcacaa
     2821 tatcaacgtt cacatgggca tgggtgaccc tcgtccagat gaactgatgc tcactgagga
     2881 cggcatcgca ttctgtcagt ccattgggat acggggaatg tctctgaact ttgggaacat
     2941 gaagaatccc tttttctatt tcctcatgga tg
//
LOCUS       DAOQUC010000356         2969 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_38961_length_2969_cov_2.422100, whole genome shotgun sequence.
ACCESSION   DAOQUC010000356 DAOQUC010000000
VERSION     DAOQUC010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2969)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2969)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2969
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38961_length_2969_cov_2.422100"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1042
                     /locus_tag="PKH07_07220"
     CDS             <1..1042
                     /locus_tag="PKH07_07220"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNT34771.1"
                     /translation="RGKASRKALTAMTRQLTTLIGAGLPLRRSLSILEEQCEDSNLRD
                     ALGGIGEDIEAGSNFSEALAKYPRIFNKLFINMVKAGELSGALEEVLDRLSIFAEKAA
                     GVRAKVRSAMMYPAFVVLFSAILVSLILIFIVPKFEAIYTGFGAKLPAMTQLLVVASG
                     ILMHHAPAVILGFVAFMILLWRVNKTEKGKYCTDYLLLKLPIFGAIIQKGCIAKFSRT
                     FATLLQTGVPILQSLVVVKDTAGNEVVARAMIRVHASIREGDTISEPLRECWVFPPLV
                     IHMIAIGEETGAIDTLLAKVAEAYEREVDAAVDGLTALIEPLIIIILGVIIGFIVIAL
                     YMPIFKLVEAVK"
     gene            1114..2550
                     /locus_tag="PKH07_07225"
     CDS             1114..2550
                     /locus_tag="PKH07_07225"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNT34772.1"
                     /translation="MKVWYLKSSRHLFWIVLVVALVVRLPFLFFTADDAFITYRHVTN
                     LLSGDGLVYNPGELVLGASSPLYAMALAALGLGGLDFILWGKLLGIVAGSLACVLLYR
                     ILRCEVGEETALLAGLLGALSPYLVVWSASGMETGTALLLALLTIDMYRQNKMVPTGI
                     FLGLSILTRFDAGLLGFVLCCHYGMKERRFPIELIASACSVVTPWLAFSLAYYGMLLP
                     NSLVAKFLVYKGTSEVTPLVEKLGVFFGKPDKFGFSLLVIWGYIRIFRTKKTSLFLLG
                     FWSLLHLCSLLLSEGCMFAWYYCPVFPGYVALGAWGVVDLCDRFASRVRQHPKTLFLA
                     TLLFLVLLQSFRLIHSWKLHQEIQAEMTQVHKQIGLWLKDKSQPKDTILVGDIGYIGY
                     YSERHILDWMGLVSPKVIPYHKNGIPEKVVLDFRPEYIVLGEYDYLFPLLTNQNWFSR
                     MYQQAQVFSEGSQVYHIWEMRSNDPGGL"
     gene            2616..>2969
                     /locus_tag="PKH07_07230"
     CDS             2616..>2969
                     /locus_tag="PKH07_07230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34773.1"
                     /translation="MVPTLAVSMLTLVCLNQSKDIGERPYEMEWANRREDAHQPLVDF
                     EYLAGWHIETRDASAELVRTREQQIWGTYVAKLTYCATGDDPEVLIRPPSKVPIHGAW
                     DALTLWVYGNNWEWAP"
BASE COUNT          636 a          743 c          769 g          821 t
ORIGIN      
        1 ccgtggcaag gcttctagaa aagcgctcac agcaatgacc cgacagctca cgaccctcat
       61 cggcgctggc cttcctctca ggcgaagcct ctcgattctg gaagaacagt gtgaagactc
      121 caatctccga gacgctctcg gcggaattgg tgaggacatt gaggcgggct caaacttctc
      181 tgaggccttg gcgaagtatc ccagaatctt caacaagctg ttcatcaaca tggtaaaagc
      241 aggagagctg agcggtgcct tggaagaggt cttggatcgt ctctcaattt tcgcggaaaa
      301 ggcagcaggt gttcgggcaa aagtccgctc ggccatgatg tatccggcat tcgttgtact
      361 gttttccgcg attcttgtgt ccttgattct catctttatc gttcccaagt ttgaggcaat
      421 ttacacaggg tttggcgcga aacttcccgc aatgacccag ttgcttgtcg ttgccagtgg
      481 aatcctgatg caccatgctc ccgccgtgat tttgggattt gtcgccttta tgattctgct
      541 gtggcgcgtg aataagactg agaagggaaa gtattgtacc gactatttgc tgctgaaact
      601 gcctattttc ggtgctatca ttcagaaagg ttgtatcgcc aagttttccc gcacttttgc
      661 aacgttactc cagactggtg ttccgattct ccaatccctc gtagtcgtga aagacaccgc
      721 tggcaatgaa gtggtcgcga gggccatgat tcgagtccat gcctcaatcc gcgagggaga
      781 cactatctcg gagccactcc gtgaatgttg ggtcttccct cccctggtca ttcacatgat
      841 tgccattggt gaagagactg gtgccattga cacactgctg gccaaggtgg ccgaagcgta
      901 cgaacgtgaa gtggacgccg cggttgatgg tttgaccgct ctaattgagc ctttgatcat
      961 tatcatcctt ggagtcatca tcggttttat cgttatcgcc ttgtacatgc ccatcttcaa
     1021 actcgtcgag gcagtcaagt aggaattggg cgtagatatc ccagaaaacc ggaatctgga
     1081 agagtctgaa tcgttttgat agactcttca gatatgaagg tttggtatct gaagtcatcg
     1141 agacatctct tctggattgt ccttgtcgtc gcgctggtcg tgagactacc gtttctgttc
     1201 ttcacggctg atgacgcctt catcacctat cgtcacgtca ccaatcttct ttcgggagac
     1261 ggacttgtct ataaccctgg agaacttgtc ctgggagcca gcagcccttt gtatgccatg
     1321 gccttggccg cattgggctt gggaggcctg gatttcatcc tgtgggggaa actcctgggg
     1381 atagtcgctg gatcgcttgc ctgcgttttg ctctacagga ttcttcgttg tgaggtagga
     1441 gaggaaaccg cactgctggc tggactactt ggcgcgctct ctccctacct cgtcgtatgg
     1501 tctgcctcag ggatggagac tgggacggcg ctcttgctgg ccctactaac cattgacatg
     1561 tacagacaga acaagatggt tccgacaggt atctttcttg ggctctccat tctgacacgg
     1621 ttcgacgcgg gacttcttgg tttcgtgctg tgctgccact atggaatgaa ggaaagacgc
     1681 tttccgatag aactgatcgc ttccgcttgt tcggttgtca ccccatggct cgccttttcg
     1741 cttgcctact acggtatgct gcttcccaat tccctggtcg ccaagtttct agtctacaaa
     1801 ggaacttctg aggttacgcc tttggtcgaa aagcttggag tgttcttcgg aaaaccggat
     1861 aagtttggtt tttcgctcct ggtgatatgg ggatacattc ggatttttcg gacgaagaag
     1921 acatccctct tcctcctggg tttctggtct ctactgcact tgtgttcgct gctgctgtca
     1981 gaaggttgca tgtttgcatg gtactactgt ccggtcttcc ctggctatgt ggcattgggg
     2041 gcttggggag tcgttgatct gtgtgatagg tttgcctctc gtgtcagaca acatcctaaa
     2101 acgttgtttt tggccacact cctcttcctc gttctacttc agtcgttccg cctgatacac
     2161 agttggaaat tgcaccaaga gatacaagcc gagatgacac aagttcacaa acaaatcgga
     2221 ctttggctca aagataaatc tcagccaaaa gacacaatcc tggtaggtga tatcggatac
     2281 atcggatact attcagaacg ccacattctg gactggatgg gtctggtctc gcccaaggtg
     2341 atcccgtatc acaagaacgg gattcctgag aaggtcgttt tggactttcg ccctgagtac
     2401 attgttttgg gagagtacga ttatctcttc cctcttctca ctaaccagaa ctggttttcc
     2461 aggatgtacc aacaagcaca agtctttagc gaaggctctc aggtgtatca tatctgggaa
     2521 atgagatcca atgatcctgg cggtctttga tggaggttgc agtaatgatg gtctctttgt
     2581 tgttgcgcca tcactgctct aggaggtaag gtgaaatggt accgactctc gccgtttcca
     2641 tgttgacttt ggtgtgcctg aatcagagca aagacatcgg tgaacgcccg tatgagatgg
     2701 aatgggcaaa tcgccgtgag gatgcccatc aaccactcgt agacttcgaa tatcttgcag
     2761 gctggcacat cgagactcga gatgcttctg cagaacttgt ccgcacccgc gagcaacaga
     2821 tatggggaac gtacgttgcg aagctcacct actgcgcaac cggtgacgat cctgaagttt
     2881 tgattcgtcc accttccaaa gttccgatcc acggtgcctg ggatgccctc accctctggg
     2941 tatatggcaa caactgggaa tgggcaccc
//
LOCUS       DAOQUC010000357         2967 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_38985_length_2967_cov_3.308379, whole genome shotgun sequence.
ACCESSION   DAOQUC010000357 DAOQUC010000000
VERSION     DAOQUC010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2967
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38985_length_2967_cov_3.308379"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..419)
                     /locus_tag="PKH07_07235"
     CDS             complement(<1..419)
                     /locus_tag="PKH07_07235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HNT34774.1"
                     /translation="MLEPGFWLMFAVRVPALLVALTVHEWAHAYTAMRKGDLTAYDAG
                     RVSLNPFVHLDPIGSIGLLLGFFGWGRPVPVDHRLLKNPTRDSMWISFAGPLSNLTLA
                     VAFGVIFRIWHLVAPPTEPVVLGEVEYLRVNLWQLSTL"
     gene            complement(602..1237)
                     /locus_tag="PKH07_07240"
     CDS             complement(602..1237)
                     /locus_tag="PKH07_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="corrinoid protein"
                     /protein_id="HNT34775.1"
                     /translation="MADLQALADQLFAGKQEEVKKLTQQAIDEGVSPKEILTNGLIKG
                     MEKVGMKFKNNEIYVPEVLIAARAMKAGMGLLKPLLAAAGHEPIAKICLGTVRGDLHD
                     IGKNLVVMMMEGAGFDVVDMGIDVKPEQFVEAAKTHNPQLIGMSALLTTTMPAMKDTI
                     DSLKAAGLTNVKTIVGGAPLTQKYADEIGASGYAPDAASAVDTAKQLLGLA"
     gene            complement(1611..2180)
                     /locus_tag="PKH07_07245"
     CDS             complement(1611..2180)
                     /locus_tag="PKH07_07245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34776.1"
                     /translation="MSTLRRMFLVVVIMQIASALSFRCALAADEGSSEEKIGIYDSRA
                     VAVAFCGSATHTKQITELDAEYERALKAGDTEKVERIKSERQGSQTRMHLQGFSTAPV
                     DDILATIEKPMEKIKTANGLSEIVSKWDKEKLKKYRNAKQLDITMDLVRAFAPNERQM
                     KSAVEIQKVKPCPMPTESGKALDAQSEHR"
     gene            complement(2660..>2967)
                     /locus_tag="PKH07_07250"
     CDS             complement(2660..>2967)
                     /locus_tag="PKH07_07250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34777.1"
                     /translation="GDDSNLVSRSELNIDAATRQWVVIRMKVSAGTFAELFYKAPKEI
                     FSAAKKKRFDLLNNTDFVTYTLDMSGESNWTGTIDHLRIDPTTAVGATVEIDYVLVP"
BASE COUNT          698 a          890 c          683 g          696 t
ORIGIN      
        1 agagtggaca actgccacaa attcacccgc aaatactcca cttcaccaag aacaacgggt
       61 tccgtcggtg gggcaaccag atgccagatt ctgaagatta caccaaacgc gacggcgaga
      121 gtcagattcg acaaaggccc ggcaaacgaa atccacatgg agtcccgagt cggattcttg
      181 agcaagcgat gatccaccgg aacaggcctt ccccatccga aaaaccccaa cagcaatccg
      241 atgctgccta tgggatccag atgcacaaat ggattcagag agacccgacc cgcatcgtaa
      301 gcggtcagat ctccctttcg catcgccgta taggcgtgtg cccattcatg taccgtgagc
      361 gccacaagca gcgccggcac tcgaacggca aacatcaacc agaaaccagg ctccaacata
      421 ctccctcgcc tcaacgtgga taacataccc ctttgtctgg gggagtacaa cacaaaccta
      481 ccataaatga aagcccctct gaagcatgaa caaacattca gagggacttg tcttgctcgt
      541 attcacatcc cagacgggtc aacaggcaga actctggaac cgccctggca tattcgtccg
      601 cttaggccaa tcccaagagt tgcttcgccg tgtccaccgc actggccgcg tcgggcgcat
      661 aaccactggc accaatctca tccgcatact tctgagtcaa cggtgcgcca ccgacaatgg
      721 tcttgacatt ggtcaggccc gctgccttga gagagtctat ggtgtccttc atagcgggca
      781 tcgtggtggt caacaacgcg gacatcccaa tcaactgggg attgtgggtc ttggctgcct
      841 cgacaaactg ctccggcttt acgtcaatgc ccatatccac cacgtcaaaa cctgcacctt
      901 ccatcatcat cacgacaagg ttctttccga tatcgtgcag gtcgccccgg accgtcccca
      961 ggcaaatctt ggcaattggc tcatggcctg ctgctgccag gagaggcttc agcaatccca
     1021 tgccggcttt catggctcga gcggcgatca gaacttcggg aacgtaaatc tcattgttct
     1081 taaacttcat cccgactttt tccatgccct tgatgagccc attggtgagg atttccttgg
     1141 gggaaacacc ttcatcaatc gcctgctgtg tcagcttctt gacctcttcc tgcttgcccg
     1201 caaagagttg atccgctaat gcctgcaaat ctgccatttg agtctcactc ctgatttctc
     1261 cccgagggga gggtttcgtc tcagtatttt tgggaagcta tcatcgtgat tgttatcagt
     1321 caagagaaaa caagaaacgg gctggtctca ataggccggg gtgagcagtc ggaacactcc
     1381 ttccgcttta tcccgcagtc tatacgcccc ccgttcgcga tccaggagtg acctgcaaca
     1441 ggtagagtgc ggtccgcaga gtagaccgca ccctatcccg ctttcgatca gaatcgtccc
     1501 acggttaacg gctctcttcg caaagccatg ctatcagaag cgttgactta ctgagaacga
     1561 ggaacaggcc tcagtggcgc agaagacagc cacccagaga gcaaagaact ctaccggtgc
     1621 tcagactggg catccagcgc tttgccactt tccgtcggca tcggacacgg cttgaccttc
     1681 tgaatctcaa ccgccgattt catctgcctt tcgttgggag cgaaggccct cacgagatcc
     1741 atggtaatgt ccagctgttt ggcattccta tacttcttca atttctcttt gtcccatttc
     1801 gagacgatct ccgatagtcc gttagccgtc tttatcttct ccatcggctt ctctatggtt
     1861 gccaagatat catccaccgg tgcggtgctg aacccttgga ggtgcatacg cgtctgactg
     1921 ccctgtctct cgctcttgat cctctccacc ttctccgtgt ctcctgcctt cagcgctctt
     1981 tcgtattccg catcgagttc tgtgatctgc ttcgtatgcg ttgccgagcc gcaaaacgct
     2041 acagctactg cacgtgaatc atagatgcct atcttctcct ctgaggaccc ttcatcagcc
     2101 gccaacgcgc accgaaaact cagcgcggat gcaatctgca tgataaccac caccagaaac
     2161 attcttctca aagtactcat ccctttctcc tccgttccgc attcagcggt ctaggctact
     2221 tgctgtacat cttcattctc accaaaacgt gtacgaccga tcacctccaa tcatagataa
     2281 gatatataat gtatatatat atactatata tgtgtcaagt aaaatccgcc caggtacgaa
     2341 atcggacagc tcggcgatcg ccgcgaccga ggagttcctc ctctcatgtc aaccagcgaa
     2401 ggattcttcg ctggatgttt ccgagggaga tgcttccgat gagtgagcac gctggctcag
     2461 ttacgctggg ggcaaactga acaccagtcc aggcgagagt gtcaatctga gcggaatccc
     2521 ttcgactacg ctcaggagat gcttcggagc aaagagcctt tgctgatggg cacggagtag
     2581 caagagagtt ccctcatctc gttccgccaa gacgaaactc catactgaaa gacatagtgc
     2641 aagagttcgg caccgtccct cagggcacca gcacgtagtc tatctcgact gtggcaccga
     2701 ctgctgtagt cgggtcgatc cgcagatgat caatagttcc tgtccagttg gattcaccgg
     2761 acatgtccag cgtataggtc acaaagtccg tgttgttcag cagatcgaac cgtttcttct
     2821 tggcagcact gaagatttcc ttcggtgcct tgtagaacaa ctccgcaaac gtacctgccg
     2881 aaaccttcat ccgaatcacg acccattgcc tcgttgccgc atcaatgttc aactccgaac
     2941 gagagacaag attcgagtcg tctcccg
//
LOCUS       DAOQUC010000358         2964 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_39039_length_2964_cov_3.850120, whole genome shotgun sequence.
ACCESSION   DAOQUC010000358 DAOQUC010000000
VERSION     DAOQUC010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2964)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2964)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2964
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39039_length_2964_cov_3.850120"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            247..1428
                     /locus_tag="PKH07_07255"
     CDS             247..1428
                     /locus_tag="PKH07_07255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34778.1"
                     /translation="MDIEVSQTTLGSRFPSISSGAQDSLQSASPSSSGGATQQFFSTQ
                     DQLEISSEARASLVALREAEAEAVSVLQEFAAQFSASYQSQTLAQVSRVANNSRSSLS
                     VLSQTTFNFSVQISASSLSTQQGGIDLSSLSDAKLKDYLILLRNFLKDMRKGMNEFFK
                     DFSTWVKGDGTMDLDKVTSFINNLAGDLEDFLGNQAAGSNVSGVAASLRNINISLEVS
                     ASNTTVVMEQIGDPLVLDLDGDGVQLTSLLDGVNFDLNGDGIQDRMAALRGADAFLAW
                     DRNGNGIIDNGKELFGDQHGSSHGFGELARFDQNRDGLIDVQDEIFSNLLLFVDTNRD
                     GVSQKSELRSLMQAGIQSLSLKYMDVQNVSRGGMVSQVGSFNRTDGTQGLLADVWLKY
                     I"
     gene            1509..2807
                     /locus_tag="PKH07_07260"
     CDS             1509..2807
                     /locus_tag="PKH07_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNT34779.1"
                     /translation="MVFADKAGEIVEHPEWEFTGQSASYFVRISEDDLIALPESSRLF
                     TLPGRFPVGWDPERRSFDIVQDMAEVYDENGLVQGVAAFLPPGYLRCVLPAFARTEEA
                     PMLPLWAYTAVGWRDGGFVVPAVQVDKLWYWEPNQFDDRKLVPLVEKRLKENPRNRLL
                     KHLSRCALTYHCFAGKNLFLQRGEAPLPIAPACNCACIGCLSLQSQQGCEASHERINF
                     VPTPEEILELAVPHLETVPDSIVSFGQGCEGDPILYGDTLVQAISGMRRATQKGTINL
                     NTNGSLPETVEKVAKAGLDSIRISMNAVSPERYARYYRPKSYAYEDVVHSLKIASDNG
                     VHVAINLLIFPGITDLRDEYDGLIKLVRECGVHMIQLRNLNIDPDLYLDAVGRPEETG
                     MGIKAFLESLKKDCPELILGYFNRPRNRFSPKSLMGIRCI"
BASE COUNT          668 a          774 c          802 g          720 t
ORIGIN      
        1 gatcctgaag tacgggaggt tttgatgaac tggtgtgcct ccacagactc ctttgaaatt
       61 ccgagtgaac catcttctca gaagccaaac atgaagaagg cctgatcttt ccaaagacat
      121 tttctttccc aaagttttct ctgcctcagt ctgccaaggc tgatactatt cggctttttc
      181 tgctcaagat agttctccaa cctgccgata ccgaatataa gtaagaagat cgcgtgtggc
      241 gggactatgg atattgaagt ttcccagaca actctgggca gtcgttttcc aagcatctct
      301 tcaggagcgc aggattccct gcagagcgcc agtccctctt cgtcaggcgg agcaactcag
      361 cagttttttt caacgcaaga ccaacttgag atctcctccg aggctcgggc gtccctggtc
      421 gcgttgcgcg aggccgaggc ggaagccgtt tcggtgctcc aggaatttgc ggcgcagttc
      481 tccgcttcgt accagtctca gaccttggca caggtctcca gagtggccaa caacagccga
      541 tcgagtctct ccgtattgtc tcagaccaca ttcaacttca gcgttcagat atccgcttct
      601 tcgctctcga cgcagcaggg aggcattgat ctttcttcgc tttcagacgc caaactgaaa
      661 gactatctta ttctgctcag gaatttcctc aaagatatgc gcaaagggat gaatgagttc
      721 ttcaaggatt tctccacgtg ggtcaaggga gacggaacca tggacttgga caaggtgacc
      781 tctttcatca acaatcttgc cggagatctg gaagatttcc tgggcaacca ggccgcgggt
      841 tcgaacgttt ccggagttgc ggcgagtctt cgcaatatca acatctctct tgaagtatct
      901 gcgtccaaca caacggtggt aatggagcag atcggggatc cgctggtgct tgatctggat
      961 ggtgatggag tacaactgac gtcgttgctt gacggagtca acttcgattt gaacggcgat
     1021 ggcatccaag accggatggc cgcactccgg ggagccgacg cttttttggc gtgggatcgc
     1081 aacggaaatg gcatcattga caacggaaag gaactctttg gcgaccagca cgggtcatcg
     1141 catggatttg gcgaactcgc gcgattcgat caaaaccgcg acggcttgat tgacgttcag
     1201 gacgagatct tcagcaactt gctgcttttt gtggacacga atcgagacgg tgtcagccag
     1261 aagagcgagc tccgctctct gatgcaggcc ggcatccagt cgctgagcct caagtatatg
     1321 gatgttcaga acgtctcacg tggggggatg gtctcccaag tcggttcctt caatcgaacg
     1381 gacggaacac aaggccttct tgctgatgta tggctcaagt acatctgagc gagtcttcac
     1441 aagaaggagc caaccagtca agactcctgt gctatagtct tctcatgagt agcagacgca
     1501 atacattgat ggtgttcgcc gacaaggcgg gtgaaatagt cgagcatccg gaatgggagt
     1561 tcactggaca gtccgccagc tacttcgtcc gcatttcgga agacgatctg attgctctgc
     1621 ccgagtcgag ccgactgttc actctgccgg gaaggttccc tgttgggtgg gatcctgaga
     1681 gacgcagttt tgacatcgtc caggacatgg ctgaggtcta tgacgagaat gggcttgttc
     1741 aaggtgtcgc cgcatttctt ccgccgggat atttgcgttg tgttctgcct gctttcgcca
     1801 ggacggaaga agcgccaatg cttcccctgt gggcttatac cgctgtcgga tggcgggatg
     1861 ggggtttcgt ggttcccgca gttcaggtgg acaagctgtg gtactgggag cccaatcaat
     1921 tcgatgaccg aaagctggtg cctctggtcg aaaagcgtct caaggagaac cctcgaaacc
     1981 ggcttctgaa gcatctgtct cgttgcgctt tgacgtatca ctgttttgcg gggaagaatc
     2041 tctttctcca aagaggtgag gctcctttgc ctattgctcc cgcgtgcaac tgtgcctgca
     2101 ttggttgcct gtcgcttcag tcccagcagg gatgcgaagc atctcacgag cggattaact
     2161 ttgttcccac accagaagag atcctggaac ttgccgttcc gcacctcgag acggttccag
     2221 acagcatcgt cagttttggg cagggttgtg aaggggatcc cattttgtac ggtgacactt
     2281 tggttcaggc aatctcggga atgcgaagag ccacacaaaa aggcaccatc aacctcaata
     2341 ccaacggcag cctgcccgaa actgttgaga aagtcgccaa agccggtttg gatagcattc
     2401 gcatcagcat gaacgcggtt tcgccggaac gctacgcccg ctactaccga ccgaaaagct
     2461 atgcgtatga ggatgtggtg cattccctga aaatagcctc cgacaacgga gttcatgtcg
     2521 cgatcaatct tctgatcttt cccggcatta ccgatctcag ggatgaatac gacgggttga
     2581 tcaaacttgt tcgagaatgc ggcgttcata tgatccaatt gaggaatctg aacattgacc
     2641 ccgatctcta ccttgatgct gtaggtcgtc ccgaggaaac cggcatgggc atcaaggcct
     2701 ttttggaatc tctcaagaaa gactgtccgg aactgatact tggctatttc aaccgaccgc
     2761 gaaatcgctt ctccccgaag tctctcatgg gcatacggtg catctgaagg gaatgacaaa
     2821 tccccccttg atcccccctt ggcaaggggg gcagtctccg gaatcccccc ttgatccccc
     2881 cttggaaagg ggggtatttt cggaaccacc ttgacgaagg gggctcttgt tctgaacccc
     2941 ccttaacaag gggggctggg ggga
//
LOCUS       DAOQUC010000359         2961 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_39104_length_2961_cov_3.662423, whole genome shotgun sequence.
ACCESSION   DAOQUC010000359 DAOQUC010000000
VERSION     DAOQUC010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2961
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39104_length_2961_cov_3.662423"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..312
                     /locus_tag="PKH07_07265"
     CDS             <1..312
                     /locus_tag="PKH07_07265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34780.1"
                     /translation="VLFLEITDWVDAVEEQQLPLMKKPVQVLVVNIALHGSVVDLQSG
                     QVLSAGSVTASSPRSEIVVDGHGDLNMQIKVSEEAADKFLGLLSADGVLQWEPQEPSQ
                     E"
     gene            373..1857
                     /locus_tag="PKH07_07270"
     CDS             373..1857
                     /locus_tag="PKH07_07270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34781.1"
                     /translation="MMRTNRLAYRFLLCVLLLPLCGCSVLTHYTDRVSPVRDVFVQGN
                     PQGALSLLQTRKVNSATDRVCYLLEEGTLKHTAGDFAGSNQIFFDAVSIMQDRDMRAV
                     VSGGKTLEQAGSVLINEKTIAYEGEGFERVFVHTLQALNFLLSNDKGSARVEIKKAYE
                     EADWLEQRNYKKLEAAQDKLQEENAKRQQQNQQLSQTAILDNANSTFSQDPDYAMLQS
                     KALSVLNAYQNAFTYYLSSLVYEMNGDYNDAYIDCKTAHQLRPGVPAVQRDLVRLAAL
                     NGFKDDLEQWEKEFGLNYAEMPMDVGDLIVVHQCGTAVEKKQIWVPIPIPEVGIQSFA
                     FAVYRPVPFPAWAVSVVDGSTTLGATDFLADMDALAVRNLNDRIPEIVIRTAVRFAIK
                     ALATYQMKKEMGAGGALIGMALTALTEQADLRSWLLMPSNLQIARIPLPPGEHRLGIV
                     LEDSRRQTLDRRDITVTIEKGKMTLVNLRGIQKIVNDVQISSSL"
     gene            1878..2309
                     /locus_tag="PKH07_07275"
     CDS             1878..2309
                     /locus_tag="PKH07_07275"
                     /inference="COORDINATES: protein motif:HMM:NF018889.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HNT34782.1"
                     /translation="MKTRVVLLTGVLLLGLVSMISGCARNTTSNVAELRTSGDGTILD
                     KRIIINHRGFDKRVAIQGLDVRKAGDILQARVVLQNNKKKTIAFAYKFEWYGQDGFPI
                     DTGTSVWKNEWVKGKDLKNLLGTAPSAKATDVRLLIQQVKK"
     gene            2347..2952
                     /locus_tag="PKH07_07280"
     CDS             2347..2952
                     /locus_tag="PKH07_07280"
                     /inference="COORDINATES: protein motif:HMM:NF024436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34783.1"
                     /translation="MRIAYRIVLLGLVVGLAGCASAPRVTEVSSMPDPEVNRLDGTWG
                     SEDVHLVVEKMVSSMISDEPIASRTDVPLVFFLGVKNMTSDHEIQPVEIEDAISVAAH
                     KSRKVKFSAVKDISDEMVDQLGFQKSALTDPETARQYGKMKGWQYTLYGQVTSIVKRD
                     PKIREKRYRMTLKLADIETGVIEWLDEKEIRLREKRSSVGW"
BASE COUNT          732 a          688 c          862 g          679 t
ORIGIN      
        1 gttctctttc tggagatcac cgactgggtg gacgccgttg aggaacagca gctgcctctc
       61 atgaagaaac cagtccaggt cttggtggtc aacattgcgc tccatggcag cgttgtggac
      121 ttgcagtccg gccaggtact ctcggcgggt tcggtcaccg catcatcgcc aagaagtgag
      181 attgtcgtgg atggccatgg tgatctgaac atgcagatca aggtctccga agaagcagcg
      241 gataagttcc tgggactgct ctctgcagac ggtgttctcc agtgggaacc gcaagagccg
      301 agtcaggagt gaggtgacac gatctgaatg ctgcagactg agttgaatcg ctgcagtgtg
      361 tttgtcagga gaatgatgcg gacgaatcgc ttggcctatc gtttcttgct ttgtgtgctg
      421 ctgcttccgc tgtgcggatg cagcgtcctg acgcattata ccgaccgggt gtctccagtt
      481 cgtgacgtgt tcgttcaggg caatcctcag ggggcgcttt cgctgcttca gactcggaaa
      541 gtgaacagtg cgaccgacag agtctgctac cttctcgagg aaggaacact aaagcacacg
      601 gctggagact tcgcgggcag caaccagatt ttcttcgatg cagtgtcgat catgcaagat
      661 agagacatgc gggcagtggt ctccggcggt aagacgctgg agcaggccgg ttctgtcctg
      721 atcaatgaaa agacaatagc ctatgagggg gaaggctttg agcgtgtctt tgtgcacacg
      781 cttcaggcac tgaactttct gctttccaac gacaagggaa gcgcccgtgt cgaaatcaag
      841 aaggcctatg aagaagccga ctggctggaa cagcgaaact acaagaaact cgaagcagct
      901 caggacaaac tgcaggagga aaacgccaaa cgtcagcaac agaaccagca gcttagccaa
      961 acggcgatcc tcgacaatgc caacagcacc ttctcccagg atcccgacta tgcaatgctt
     1021 cagagcaagg ctctgtcggt cttgaacgct taccagaatg cgtttactta ctacctctct
     1081 tcactcgtgt acgagatgaa tggtgactac aacgatgcct acattgactg caagacggcg
     1141 catcagttga gacccggagt gcctgcagtg cagagggatc tcgttcgctt ggcggcactg
     1201 aatggcttca aagatgacct ggagcaatgg gagaaagagt ttggcctcaa ctatgccgag
     1261 atgccaatgg atgtgggtga tctcattgtt gtgcatcagt gtggaactgc tgttgagaag
     1321 aaacagattt gggttccgat tcccatcccg gaagtgggaa tacagtcatt cgcgtttgcg
     1381 gtgtaccgac ccgtgccttt tcccgcgtgg gctgtgtcgg tcgtggatgg ctccacaacg
     1441 ctcggagcaa ctgatttcct ggcggatatg gatgccttgg cagtgaggaa tctcaatgat
     1501 cgaattcctg agattgtgat tcgaactgcg gttcgatttg ccatcaaagc cttggcaaca
     1561 taccagatga agaaagaaat gggagccggt ggagcgctca ttggaatggc actgacagcg
     1621 ctgaccgaac aagccgacct gcgcagttgg cttctgatgc catcgaacct tcagattgcc
     1681 cgcatcccgt tgccacccgg ggaacatcgt ctgggcattg tgcttgagga ctcccgcaga
     1741 cagacattgg acagacggga catcaccgtg actattgaaa agggaaagat gacgcttgtg
     1801 aatcttcgag gaattcaaaa aatagtgaac gatgttcaga tttcatcgtc tctatagaga
     1861 gtgaaaggag tcgaaggatg aagacgagag ttgttttgct gacaggtgtc ttgctcttgg
     1921 ggttggtttc gatgatctcg ggctgcgctc gcaatactac cagcaatgtt gctgagttgc
     1981 ggacgagcgg cgacggtacc atacttgaca agcggatcat catcaaccat cgtggctttg
     2041 acaaacgggt tgccattcag ggtctggatg tccgaaaggc cggagacatt cttcaggcgc
     2101 gcgtggttct tcagaacaac aagaagaaga ccattgcctt cgcctacaag tttgaatggt
     2161 atggtcagga tggctttcct attgacacgg gaaccagtgt ctggaaaaac gaatgggtga
     2221 agggcaaaga cctgaagaac ctgcttggca cagcgcccag tgccaaagcg acggatgttc
     2281 ggttgctgat ccaacaggtg aagaagtagt cagatgaagt attgttgtga aaggagttca
     2341 ctgcaaatga gaatcgcgta tcgaatcgta ttgttaggtc ttgttgtggg gttggcaggt
     2401 tgcgccagtg ctccacgtgt taccgaagtg tcttcaatgc cggatcctga agtcaaccgt
     2461 ctggacggca cctggggctc agaagacgtg catctggtcg tggagaagat ggtttcatcc
     2521 atgatttcgg atgagcctat tgccagtcga acggacgttc cactggtatt ctttctcggt
     2581 gtcaagaata tgaccagcga tcacgagatt cagcccgtag aaattgaaga tgccatttcg
     2641 gtggcagctc ataagtcacg aaaagtgaaa ttcagtgccg tcaaagacat ctccgacgag
     2701 atggtggatc agcttggatt tcagaagtcc gcgctcactg atcccgaaac agcccgccag
     2761 tatggcaaga tgaagggctg gcagtacacg ctctacggac aggttacgtc aatcgtcaaa
     2821 cgtgatccaa aaattcgcga gaagcgctat cggatgacgc tgaaactggc ggatattgag
     2881 actggtgtga ttgagtggct ggatgaaaag gaaattcgcc ttcgcgagaa gcgtagcagt
     2941 gtaggctggt aagagcagct g
//
LOCUS       DAOQUC010000360         2957 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_39186_length_2957_cov_3.031702, whole genome shotgun sequence.
ACCESSION   DAOQUC010000360 DAOQUC010000000
VERSION     DAOQUC010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2957)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2957)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2957
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39186_length_2957_cov_3.031702"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..494
                     /locus_tag="PKH07_07285"
     CDS             <1..494
                     /locus_tag="PKH07_07285"
                     /inference="COORDINATES: protein motif:HMM:NF014867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HNT34784.1"
                     /translation="RFRYLLSRHCRVDSRNVHAYILGEHGDSEVAAWSLTHIAGIQMD
                     DCCRVCPRQCGLVERAGIVEQVKNSAYHIIDAKGFTNYAVGLAMVRIVQAVLRNENSI
                     LTVSTLLGGEFGIEDVCLGVPTVVNANGADMIFDAPLPDGERKALQASADCIRQIIRQ
                     VGF"
     gene            554..1129
                     /locus_tag="PKH07_07290"
     CDS             554..1129
                     /locus_tag="PKH07_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HNT34785.1"
                     /translation="MASLKGSKTEKNLLAAFAGESQARNRYTYFAGQAKKEGLEQIAW
                     VFGETADQEKEHAKRFFKFLEGGDVEIVAAYPAGVIGTTAENLKAAADGEQHEWTTLY
                     QNFSKQARDEGFDMVVKAFESILIAEKFHEKRYRGLLKNIEAGTVFKKAQPVVWRCRN
                     CGFIHEGNEAPNVCPACLHPQAFFELSLENW"
     gene            1174..1548
                     /locus_tag="PKH07_07295"
     CDS             1174..1548
                     /locus_tag="PKH07_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007292452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HNT34786.1"
                     /translation="MAQKFEIYKCEKCGTIVEVLHGGVGQLVCCGEPMKIYVENTVDA
                     AKEKHVPVIEKGCCEVKVKVGSVAHPMEEKHYIEWIEVIADDKVHRQFLKPGQAPEAT
                     FQVKADVITVREYCNLHGLWKA"
     gene            1645..2163
                     /gene="tpx"
                     /locus_tag="PKH07_07300"
     CDS             1645..2163
                     /gene="tpx"
                     /locus_tag="PKH07_07300"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012765903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HNT34787.1"
                     /translation="MANQEKGSVTFQGNPLTLIGDEIQVGQPLPDCELLDKGLGAVKL
                     SSYRGKVCVISTVPSLDTSVCDLQTRRFNQEAAALGEDVVILTVSMDLPFAQARWCGA
                     AGVDRVITLSDHREAVFGKACGLLIKELRLLARAVLVVDRQGTVRSVEIVREITNHPD
                     YENAVKAVKSLL"
     gene            2228..2752
                     /locus_tag="PKH07_07305"
     CDS             2228..2752
                     /locus_tag="PKH07_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HNT34788.1"
                     /translation="MLSKKMQKALCDQVTAEHYSAYLYLGMSAYFNSIDLPGFANWMY
                     IQAQEELVHGIAIYHQIHERNGEVTLNTIEAPPKKWKSPVDVFENTCSHEQKVTASIN
                     NLVNLAIEEKDHATNNFLQWYVKEQVEEEATAYAILQKLKMMKDAPGGIFMMDKELGA
                     RVFTMPSIMAASAD"
BASE COUNT          732 a          666 c          907 g          652 t
ORIGIN      
        1 cacgttttag atacctgttg agccgccact gtcgcgttga ctcccgcaac gttcatgcat
       61 acatcctggg agaacatggt gacagtgagg tggccgcatg gagtctgacg catattgccg
      121 gaattcagat ggatgattgc tgccgcgtgt gtccgaggca atgtggcctg gttgagcgcg
      181 caggtattgt ggagcaggtc aaaaactccg cgtatcacat cattgacgcc aagggcttta
      241 ccaactatgc tgttggtttg gcgatggttc ggattgttca ggcggttctt cggaacgaga
      301 acagcatcct gaccgtttcg acattactcg gcggagaatt cggaattgag gatgtctgtc
      361 tgggagttcc gacagtggtc aacgccaatg gagcggacat gatttttgat gcgccgcttc
      421 cggatggtga gagaaaggcg cttcaggctt cggcggactg tattcgccag atcatccgac
      481 aagtcggttt ctgaaacggt ctatccgttt gaacatagtc tagaaacagg acaacaataa
      541 ggaggaagag aacatggcat cgttgaaagg gtcgaagacg gagaagaact tgctggcggc
      601 ttttgccggg gagtcccagg cgaggaaccg ctacacctac tttgccggtc aggcaaagaa
      661 ggaaggactt gagcagatcg cctgggtttt tggagagacg gcggatcagg agaaggaaca
      721 tgcaaagcgt ttcttcaagt tccttgaagg tggggacgtg gagatcgtcg ccgcctatcc
      781 tgccggcgtg attggaacca cggcggaaaa cctcaaggct gctgccgatg gggagcaaca
      841 tgagtggacg acgctttatc agaacttctc gaagcaggcc agggatgaag ggtttgacat
      901 ggttgtcaag gcctttgaga gcattctgat tgctgaaaag ttccacgaga agaggtatcg
      961 cggtcttctg aagaacatcg aggcgggcac agtgttcaag aaggcgcaac cggttgtctg
     1021 gagatgtcgc aactgcgggt tcatccatga aggcaacgaa gctcccaacg tctgtccggc
     1081 atgtctgcat ccgcaggcgt tcttcgagct gtccttggag aactggtagt cccggatagg
     1141 gtttgagaat ccatcgaatg aggaggagaa gagatggcac agaaattcga aatctacaag
     1201 tgtgagaagt gcggaaccat tgtggaagtt cttcacgggg gcgtagggca gctggtgtgc
     1261 tgtggcgagc cgatgaaaat ctacgtggag aacacggtgg atgctgccaa ggaaaagcac
     1321 gtccctgtga tcgaaaaggg atgctgcgag gttaaggtca aggtcggcag tgttgcccat
     1381 ccgatggagg aaaagcacta catcgaatgg atcgaggtga tcgcggacga caaggtgcat
     1441 cgtcagtttc tgaaaccggg tcaagccccc gaggcgacgt tccaggtaaa agctgacgtg
     1501 atcacggttc gcgaatactg caaccttcac ggtctttgga aggcctgatt gaaaagctga
     1561 agatgtgctg gcggggtgac ggctgagact ctcctcatcc cgccggacag acagcgggca
     1621 gagtcaattg agaggaggag tcctgtggct aatcaggaaa aaggttctgt gacgtttcaa
     1681 ggcaatccgc tgaccctcat tggcgatgag atccaggtgg ggcagccgtt gcctgactgt
     1741 gagctgttgg acaagggtct tggtgcggtc aaactctcca gttacagggg aaaagtatgc
     1801 gtcatctcga cggtgccttc actggacacc tcggtctgtg atctgcagac gcggcgtttc
     1861 aatcaggaag ccgccgctct gggagaggat gttgtcatac ttacagtcag catggatctt
     1921 ccgttcgcgc aggctcgttg gtgtggtgct gcaggggtag accgagtgat caccttgtcc
     1981 gatcaccgag aggcggtttt cgggaaggcg tgcgggctgc tcatcaagga actgagactt
     2041 ctggcgagag ccgttcttgt ggtcgatcgg caaggaacgg ttcgctctgt tgagattgtc
     2101 cgagagatca ccaatcatcc ggactatgaa aacgcggtga aggctgtcaa aagccttctg
     2161 tgaagcaatt cgaaacaagg gcgaggttgt ttatttcctt tgcccatcaa gaggaggaag
     2221 aggacttatg ttgagcaaga aaatgcagaa agcactttgt gatcaggtca cggcggagca
     2281 ttattccgca tatctgtatc tggggatgtc agcctatttc aactcgattg atcttcccgg
     2341 gtttgccaac tggatgtaca ttcaggctca ggaagaactg gttcacggca ttgccattta
     2401 ccatcagatc cacgagagga atggcgaggt gacgctgaac acgatcgagg ctcccccaaa
     2461 gaagtggaag tcgccagtgg acgtttttga gaacacctgt tcccatgagc agaaggtcac
     2521 ggcgagcatc aacaacctgg tgaatctggc tatcgaagag aaggatcacg ccaccaacaa
     2581 tttccttcag tggtatgtca aggaacaggt tgaggaagaa gcaacggcat acgcgattct
     2641 ccagaaactc aagatgatga aggatgcccc tggtggtatc tttatgatgg acaaggaact
     2701 cggcgcacga gtattcacga tgccgtccat catggcggcg tctgcggact gaagaaggcg
     2761 tgtgctttgg caccaaggtt tcgagaggca taattgactg tgtactacag tcgtgccttc
     2821 cagattggag aaaaggaata tggccgaaac aaagcaacag attctggaca cagcgttgaa
     2881 actggagcag gacggtcatg cgttctacat ggacgctgcc aactcggctt ccaacccgct
     2941 ggccaagaag atgtacc
//
LOCUS       DAOQUC010000361         2952 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_39284_length_2952_cov_3.642043, whole genome shotgun sequence.
ACCESSION   DAOQUC010000361 DAOQUC010000000
VERSION     DAOQUC010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2952)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2952)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2952
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39284_length_2952_cov_3.642043"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..423
                     /locus_tag="PKH07_07310"
     CDS             <1..423
                     /locus_tag="PKH07_07310"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNT34789.1"
                     /translation="VFVEQLLQGMDYEIRIAGADVSLDVLPPCRADAAQLSQVFSNLV
                     SNALKYLDPKRRGVIRISGSEAADGVVYRIQDNGVGIAPQDWDRIFDPLVRVEGRDAD
                     GEGLGLTVVKTVVERHGGKVWLESAPGKGSTFFISLPR"
     gene            442..900
                     /locus_tag="PKH07_07315"
     CDS             442..900
                     /locus_tag="PKH07_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017773783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNT34790.1"
                     /translation="MNREVEIILVEDDEGHVSLVQRNLRRAGILNTIHCFQDGADVLD
                     FLFRKGKPPHRKSGVPYLLVLDIRMPKVDGVEVLRELKQDPELRKIPIIMLTTTDDPR
                     EVERCHALGCSHYITKPIDYDTFINAIQQLGLFLMVVEVPKINGEYAAST"
     gene            945..>2952
                     /locus_tag="PKH07_07320"
     CDS             945..>2952
                     /locus_tag="PKH07_07320"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNT34791.1"
                     /translation="MKERIRIPRLAMLVSGFILVAMLGCKAAKTAPNVLLITLDTVRA
                     DRLGCYGYGDIKTPNIDQLAREGILFEKAYCQVPITLPSHASILTGLYPPNHGIRLNG
                     PYLLPSDVPTVSSLLQEKGYRTGAFVSASVLLSQFGMSRGFDIYDDEILSASTAYMPE
                     RRAAVTGQRAVNWLKSSSMDSFFLWVHFFDPHMPYDPPEPFRSQYEANPYDGEIAYVD
                     SVLGKVLGALEETGERDNTLIILTADHGEGLGEHEETTHSLFVYDGTVRVPLILVLPK
                     NQVKDSSPWKPGTRVSQVVETVDIVPTVLDVLGMKTALRFDGKSLLNADKETSRYAYA
                     ECLYPMSMGWCPLQTARQDQWKYIRAPRRELFDTDTDPSERANLYSDDHERARDLEKW
                     LSGYPIYSEREASLKQNVSPEVARSLTDLGYLSLGTTVRRSEESLFKLPDPKDRTAVF
                     EQINKAPYLMNTGHAQEALDLLIPLLKGGEHSNPVLNRMLGINYVKMGLFETGLPFLK
                     AYRLLAEGDLESSYYLGVALLRSGSTEAALAPFRECVEAMPSYAAAWDQLAVAYGMQK
                     DYDKALEAGTKAVEREPLNPEYLKNLGVTLLRKGLPDQAETHFAKALEVQPDSADLHH
                     HSGLALKFQGKNEQAARSFEKALTLQPDFVDASQYLVEVLFKMGN"
BASE COUNT          708 a          743 c          816 g          685 t
ORIGIN      
        1 gtctttgtgg aacagctgct gcagggaatg gactatgaga tcagaatcgc gggggcggat
       61 gtgtctctgg atgtgctccc gccctgtcgg gcggatgccg cgcaactgag ccaggtcttc
      121 agcaatcttg tcagcaacgc cctcaagtat ctcgacccga aacggcgcgg tgtgattcga
      181 atctccggat cggaagccgc cgatggggtc gtctatcgta ttcaagacaa tggagtcgga
      241 attgctccac aggactggga tcggattttt gatcctttgg tccgagttga aggtcgagat
      301 gcagatggcg aaggtcttgg attgactgtg gtcaagactg tggtagaacg tcatggagga
      361 aaggtttggc tggaatcagc tcctggcaag ggaagcacgt ttttcatttc cctgccgaga
      421 tagaggtttg gaggaatcat gatgaaccgg gaagtcgaga tcatcctggt cgaagacgat
      481 gaggggcatg tctctttggt tcagagaaac ctgagacgag cgggaattct caataccatt
      541 cactgttttc aggatggagc ggatgtactt gacttcctgt ttcgaaaggg aaagcctccg
      601 caccgaaaat caggcgttcc ctatctcctg gtactggaca tcagaatgcc caaagtggat
      661 ggtgtcgagg tgcttcggga gctcaaacag gatcccgagc tacggaaaat cccaattatc
      721 atgctgacta cgacagacga tccccgcgaa gtggaacgct gccacgcctt gggatgcagt
      781 cactatatca ccaagccgat tgattacgac accttcatca atgccattca gcagctaggg
      841 ctttttctta tggttgtcga ggtgccgaag atcaatggag agtatgctgc gagcacatag
      901 ccgatagact cacgatcggc ggagagaaca caaaggacag agagatgaag gaaagaatcc
      961 gcattccacg tctggcgatg ttggtgtcag gtttcatcct ggtggcgatg ctcgggtgca
     1021 aggcagccaa aactgcgccc aacgttctcc tgatcactct tgacactgtt cgtgctgacc
     1081 gactgggttg ttacggatat ggcgacatca agacgccgaa catagaccaa ctggcaagag
     1141 aagggatcct tttcgagaag gcatattgcc aagttccaat cacgctccct tcgcacgctt
     1201 ccatcctgac tggcctctac cctcccaatc atggtatcag gctcaatggc ccctatctgc
     1261 ttccctcgga tgtgcccacc gtttcctctc tccttcagga gaagggttac cgaaccggcg
     1321 cctttgtaag tgcgtcggtg ctcttgtccc agttcggcat gtccagagga ttcgatatct
     1381 atgacgacga gatactgagt gcatcaacag cctatatgcc cgagcgtcgc gcagcggtaa
     1441 cgggacagcg tgccgtgaac tggctgaaat cgagcagtat ggactctttt ttcctctggg
     1501 ttcatttctt tgatccacac atgccctatg atccgcctga gccatttcgc agccaatatg
     1561 aagccaaccc ctatgatggg gaaatcgctt atgtggacag cgtcttgggc aaggtacttg
     1621 gagcccttga agagacaggt gaaagagaca atacgctgat catcctcaca gcggatcatg
     1681 gagaaggctt gggagaacat gaggaaacca cacattcact tttcgtatac gacggtactg
     1741 tccgcgttcc cctgatcctg gttctcccga agaatcaggt caaggattcc agcccctgga
     1801 aaccgggcac acgtgtcagt caagttgtcg aaactgtaga tattgtcccc acagttctcg
     1861 acgtattggg aatgaaaacc gcacttcgct ttgacggaaa aagtcttctg aacgccgaca
     1921 aagagacgtc ccgctatgcc tacgctgaat gcctttaccc gatgtccatg ggttggtgtc
     1981 cgcttcagac agcgcgccag gaccaatgga agtacattcg ggcaccacga agggaattgt
     2041 ttgataccga cactgatccc tccgagcgcg caaatctcta cagcgacgac cacgagcggg
     2101 cgagggatct cgaaaaatgg ctctctggct atcccatcta ttctgagcgc gaagccagtc
     2161 tcaagcagaa tgtgtcaccg gaagtcgcca ggagtcttac cgatcttggc tatctttcac
     2221 tgggaacaac tgtccgtcga agtgaggaga gccttttcaa actccccgat cccaaggatc
     2281 gaaccgcggt gtttgaacag atcaacaagg ctccttatct gatgaatacc ggccatgctc
     2341 aagaggcttt ggatttgctg attcctctgc ttaaaggtgg ggagcacagc aatccggtgc
     2401 tcaacaggat gctggggatc aactacgtca agatggggtt gtttgaaacg ggacttccct
     2461 ttctgaaggc ctatcggctc ttggcggaag gggacttgga gtcatcatac tatcttggcg
     2521 ttgccttgct gcgtagcggt tcgacagaag ctgcgcttgc gcctttcagg gaatgtgtcg
     2581 aggcgatgcc gagttacgct gcggcgtggg accaacttgc ggttgcctac ggcatgcaga
     2641 aagactacga caaggctctc gaagcgggca caaaggcggt cgagagagaa ccactgaatc
     2701 cggagtacct gaagaatctc ggagtcaccc tgctcagaaa aggtctgcct gatcaggcag
     2761 agactcattt tgcaaaggcc ttggaagttc agcctgattc tgctgatctg caccatcaca
     2821 gtggtcttgc actgaagttt cagggtaaga atgaacaggc agcgcgttca tttgagaaag
     2881 ccctgacact gcagccggat ttcgttgatg ccagtcagta cttggtggaa gttctgttca
     2941 agatggggaa ta
//
LOCUS       DAOQUC010000362         2945 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_39409_length_2945_cov_3.753633, whole genome shotgun sequence.
ACCESSION   DAOQUC010000362 DAOQUC010000000
VERSION     DAOQUC010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2945)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2945)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2945
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39409_length_2945_cov_3.753633"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1492
                     /locus_tag="PKH07_07325"
     CDS             <1..1492
                     /locus_tag="PKH07_07325"
                     /inference="COORDINATES: protein motif:HMM:NF021351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GldG family protein"
                     /protein_id="HNT34792.1"
                     /translation="VVTNFRAVRDSLKSRNTRHGIGQLLLFLCVSVTMCFLLLLVNSH
                     RQRFDFTQSRRYTLDAATRKVLANLQDDIQIVCCYGMGDMMWRLTHEKLIAQYSALSD
                     RVKYEFINPTKEPEKVLLFRDELIEDALPAIFVRNGSRFERARDAKEQYITNAIIRVT
                     RKGTQAVYFLQGHGERDIHSTRDAYALGFVKTKLEEKGYAVKELDMQRQSAVPTDCSV
                     LIIAAPQTDLPAFEIEAIGLYLETGGSVLILLEPETVPDLVEWLTQFGVLVGNDIVCE
                     TEIKRFLAGGRPRGEVSTSIRLTLTRKMYSPSHPITRDFQLSTEYVGCRSVSTTSTGR
                     EGIEVLKLLETKGYMEDLKMQGSWAETNIDLLRKDKAEYNEGQDIPGPVTIAAAVSLY
                     PASAEAEHKDRQRPPKRGSLIVFGDADFPTNGSRGYGGAQLFLNSVSHLCGEEDLVNV
                     RHKSIEYTDLEMTPAQRRTVSVVAVFVLPLVLIIPGTLVWLKGRRA"
     gene            1489..2868
                     /locus_tag="PKH07_07330"
     CDS             1489..2868
                     /locus_tag="PKH07_07330"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HNT34793.1"
                     /translation="MRRKTLLVAFLIMVSVIILHFWDQSRIFETKRQEQTEKRLTWGT
                     HEDIGSVEIHSTTETIQIARDTKGWRLNKPCLDLADEWQVRVLLDSLLYAEIEEIVAE
                     SSHNLANFGLEPPRIAVDLRGRAGQVLSTLLFGDRTPTGTSCYAMLKGDPRVFTLSVE
                     NYYVVSRPASEIREKTVFRHDRDNICRLEVAWGNGEGVAVSRDESGLWRLIVPTMATA
                     EQDKINVFLGKITGLRVSRYLSDVTADAAKYGLVEDVTRVTVADETSETTLTLGAWHQ
                     EGIHAARREDTASVIGIESRFVESVPTHSLDWRPQRILPVPPFQVSLLRLENPLTESV
                     LEMQKMTDGIWKVIKPEHKTDTVLDSERVSQYLSIIYDYRILDYLPNSSILPDTGLDP
                     SALRLTTGSRYQSATLVLSTISDDSKNLRATFQGSEEIFLLDKRLSDLLSVSTEEIIA
                     RLRASDASE"
BASE COUNT          729 a          761 c          798 g          657 t
ORIGIN      
        1 tgtggtcacc aatttcaggg cagtcagaga ttccctcaag agtcgcaaca cacgccacgg
       61 aattggtcaa cttctcttat tcctttgtgt ctccgtgacc atgtgcttct tgcttttgct
      121 ggtcaacagt caccgacagc ggtttgactt cactcagtca agacgatata cgctggatgc
      181 agcgacccga aaggttctag cgaatctgca agacgacatt cagattgtct gctgctacgg
      241 aatgggcgac atgatgtgga gactcaccca tgagaagctc atcgcccaat acagcgctct
      301 atcagacaga gtgaaatatg aattcatcaa tcccacaaag gaacccgaaa aggtgctgct
      361 gttcagagat gaactcatcg aagatgccct tcccgcaatt ttcgttcgaa atggctctcg
      421 atttgagcga gcaagagacg ccaaggaaca gtatattacc aatgctatca tcagggtcac
      481 tcgcaaaggc acgcaagcag tctacttcct tcaaggccac ggtgaacgcg acatccacag
      541 cacacgagat gcgtacgcgc ttggcttcgt caagaccaag ctcgaagaaa agggctatgc
      601 agtcaaggaa cttgacatgc agcgccaatc tgccgtacca acggattgct ctgtcctcat
      661 cattgcagca ccgcagacag acctcccggc ctttgaaatc gaggcaatcg gactctatct
      721 ggaaacgggc ggaagcgttc tgatcctctt ggagccggag acggttccag atttggtgga
      781 gtggttgacc cagtttggtg tgttggtggg caatgacatc gtgtgtgaaa ccgagatcaa
      841 gcgctttctc gccggtggca gaccaagggg tgaggtatcc acatccatcc gcttgaccct
      901 cacacggaaa atgtattcgc caagtcatcc catcacgcgc gacttccagc tgagcacaga
      961 atacgttggg tgccgctcgg tgagcacgac ttcgacaggg cgcgagggaa tcgaagtgct
     1021 caaactgctt gagaccaaag gatacatgga agacctgaag atgcagggca gctgggcgga
     1081 gacaaacatt gatctcctga gaaaggacaa ggccgagtac aatgagggtc aggatatccc
     1141 tggaccagtg acgattgcgg ctgccgtttc cctatacccc gcctctgcag aggcagagca
     1201 caaagatcgc cagcgtccgc cgaagagagg cagtctgatc gtctttggag atgccgattt
     1261 ccccactaat ggaagtcgag gatatggtgg tgcgcagctg ttcctcaaca gtgtcagtca
     1321 cctctgcgga gaagaagacc ttgtcaacgt gcgccacaag tccatcgaat acaccgatct
     1381 ggaaatgact ccagcgcagc gacgtaccgt gagcgtggtc gccgtctttg tcctgcctct
     1441 ggtgctcatc attccaggaa cgctggtttg gctgaaggga aggcgagcgt gaggcgcaag
     1501 acacttctcg tggcattcct gatcatggtc tctgtgatta tcctgcattt ctgggatcag
     1561 tccaggatat tcgagaccaa gaggcaagag cagacagaga agagactcac ctgggggact
     1621 cacgaggaca taggttcggt ggagattcat tcgacaacag agacaattca gatcgcacga
     1681 gacacgaagg gatggagact gaacaagccc tgtctggatt tggctgatga gtggcaagtg
     1741 cgtgtgctac tggacagtct tctctatgct gagatcgagg agattgtcgc cgaatcctcc
     1801 cacaaccttg caaacttcgg gctcgagcct cctcggatcg ctgtggacct tagaggcaga
     1861 gctgggcagg ttctttctac gttgttattt ggcgaccgga ctcccactgg caccagctgc
     1921 tatgccatgc ttaaaggtga tccacgggtc ttcactcttt ccgtggagaa ctactatgtg
     1981 gtttcaagac ctgcctcgga gatcagggag aaaaccgtct ttcggcacga tagggacaat
     2041 atctgccgcc ttgaagtagc ttggggaaac ggagaaggcg tagcagtctc gagagacgaa
     2101 tcaggtttat ggcgtctgat cgtccccacc atggcaactg ctgaacagga taagatcaac
     2161 gtctttctgg ggaagattac cggcctgcgt gtcagtcgct atctctcaga tgttacagcg
     2221 gacgctgcca agtatggact cgttgaagac gttacccggg tgacggttgc cgatgagacg
     2281 tccgaaacga cattaactct cggcgcttgg caccaggaag gcattcatgc ggcaaggcgg
     2341 gaagatactg caagcgtgat tggcatagag agtcgctttg tcgaatcggt gcccactcac
     2401 agccttgatt ggcgtcccca gaggattctc cctgttccac cctttcaagt ctccttgctg
     2461 agacttgaga accccttgac ggagtcagtt cttgagatgc agaaaatgac cgatggcatt
     2521 tggaaagtca tcaagccaga acacaaaacg gacaccgtcc tggattccga aagggtatcc
     2581 caatatctct ccattatcta tgattacaga attctggact acctgccaaa cagttctatt
     2641 ctgccggata ctggattaga tccatctgcc ttgcgactga caacggggag cagatatcag
     2701 tctgcaactc tcgttctcag caccatcagc gacgacagta agaatctccg tgcgaccttt
     2761 cagggctccg aggaaatctt tctgctggac aagaggttgt cggatttgtt gtcggtctcc
     2821 accgaggaga tcattgcacg actgcgcgcg tccgatgctt cggaatgacg taaccagaaa
     2881 agcaagcccc ggggtggaaa tcccggggcc gcagttcagt agttgtatgc gctgaccttt
     2941 gagaa
//
LOCUS       DAOQUC010000363         2944 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_39427_length_2944_cov_3.012807, whole genome shotgun sequence.
ACCESSION   DAOQUC010000363 DAOQUC010000000
VERSION     DAOQUC010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2944
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39427_length_2944_cov_3.012807"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>481
                     /locus_tag="PKH07_07335"
     CDS             <1..>481
                     /locus_tag="PKH07_07335"
                     /inference="COORDINATES: protein motif:HMM:NF024572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNT34794.1"
                     /translation="THRMEGDKQKAAEAYMQIIKRYPNSVQAEEAWLNLADLYFAEER
                     YSQALEAYSNVLKVQDRRTVTADGLADYPLGEARVKAELRIAECLRKLERLRETQESL
                     LTLQSRYPAGEIAEQISLALADLYFEWGTRENNLELFDQGIREGEKFLENYPNSQKAS
                     "
     assembly_gap    482..491
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <492..2103
                     /locus_tag="PKH07_07340"
     CDS             <492..2103
                     /locus_tag="PKH07_07340"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNT34795.1"
                     /translation="ELLGDLYHQCGLRDRVERSKYFTSALEKYQKAGEYHKADPLLPF
                     VPATLLSKEGDVRMDLGQYPEAQDLYQQVLASARLASGTVEATIKLGRSLLRQGKFSE
                     SAKVLERQLEGSVLTEQQESRACYLLGDAYYESKRYDMMMEAYGRALRLEEYVAADED
                     IDPMRAHMRMTNFLYLAQGKYKEAVAQYEEILKKYPQGRYRFRTIYYLGQAYSAMNNL
                     NEAARYFTMLIGENPELRYVDPAFLKDAYFKLGRIHFQKKDYPKAIKAYEALMERYPD
                     DPLVPSILERQGDCYAQLALYAKAEEVYLQVLDNFPESSNWSLTALKAGQLKENLSEF
                     AAASEFYARIKRRDPSDPLCTESEYRRLEMELRMAQTEPGVAAERRFASVLRDAVQAQ
                     SEYPDEIRFYAQQGVAAYSLKDFSTAADAFVKYLDQSGDLERIPRCTFLLGDALLQLE
                     RHQEAADILQKRIEMEGDDDERAHALFLLAVCQKKLNEFDKAIVTLNELLTSHPSSSF
                     IPEASAEKSRLEWSSRNVGYLQGTSQAP"
     gene            2289..2690
                     /gene="flgB"
                     /locus_tag="PKH07_07345"
     CDS             2289..2690
                     /gene="flgB"
                     /locus_tag="PKH07_07345"
                     /inference="COORDINATES: protein motif:HMM:TIGR01396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009429 - bacterial-type flagellum basal
                     body, proximal rod [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HNT34796.1"
                     /translation="MYFERIIPWSARLLREGMSQTSFRHNLITHNIANVDTPGFRRKD
                     VTFDEALKRATMELVSQAQSGRAFSSGQSTARLSVFEDDRPMLRQDGSNVDIDEEMAL
                     MAMNAGDYMRYAELHDRQMRLIRAAISERMP"
     gene            2687..>2944
                     /locus_tag="PKH07_07350"
     CDS             2687..>2944
                     /locus_tag="PKH07_07350"
                     /inference="COORDINATES: protein motif:HMM:NF012674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body protein"
                     /protein_id="HNT34797.1"
                     /translation="MSGFLISASGLTAQRTRMNVIAENLANASTTHPTPYRRKIVLFE
                     ERLAEAMDSSQSRSGAGGVRVKSIVEDNGRNAFRYLYDPDHP"
BASE COUNT          739 a          710 c          821 g          664 t
ORIGIN      
        1 gacgcacagg atggagggag acaaacagaa ggctgccgag gcctacatgc agatcatcaa
       61 gcgctatccc aacagcgttc aggcggaaga ggcatggctg aatctggccg atctctattt
      121 tgcggaagaa cgctacagcc aggctttgga agcctattcg aacgtgctga aggtgcagga
      181 caggcggaca gtgactgccg atggcctggc cgactatccg ctcggtgaag cacgggtgaa
      241 agcagaactc agaattgctg aatgtctgag aaaactggag cgtcttcggg aaacacaaga
      301 gtccttgtta acccttcaaa gtcggtatcc agcaggggag atcgccgagc aaatctcttt
      361 agcattggcc gatctttact ttgagtgggg aaccagggag aacaatctgg agttgttcga
      421 tcaagggatt cgcgaagggg agaagttcct ggagaactat cccaattccc agaaagcctc
      481 tnnnnnnnnn nggagttgct tggcgacttg taccaccaat gcggtctcag agatcgggtg
      541 gagcgaagca agtattttac ctcggcgttg gagaagtatc agaaggcagg agagtatcac
      601 aaagcagatc cgctcttgcc gtttgttcct gctacattac taagcaagga aggcgacgtt
      661 cgaatggatc ttgggcagta tccggaagcc caggatctct accaacaggt tcttgcgagt
      721 gcccgcctgg cgtctggcac ggttgaagcg acgatcaaac ttggtcgttc ccttctccgg
      781 cagggcaagt tttccgagag tgccaaagtc cttgaaagac aactcgaagg cagcgttctg
      841 accgaacaac aggaatcacg tgcctgctat cttcttggtg atgcctacta tgaatccaag
      901 cgctatgaca tgatgatgga ggcctacggc agagcactgc gtttggagga atatgtggcc
      961 gcggacgaag acattgatcc catgcgtgct cacatgcgga tgacgaattt tctctatctc
     1021 gctcaaggaa agtacaagga agctgttgcg cagtatgagg aaatactcaa gaagtatcct
     1081 caaggccgct accgattcag aaccatctac tacctgggac aagcctattc cgccatgaac
     1141 aatctcaatg aggccgcccg ttatttcacc atgctcatcg gcgagaatcc cgaactgcgc
     1201 tatgtcgatc cagcgtttct caaagatgcc tacttcaaac tggggcgcat ccatttccag
     1261 aagaaggact atcccaaggc aataaaagcc tatgaggcac tgatggagcg atacccggac
     1321 gatccactgg ttccttcaat tctggaacgt cagggcgact gctatgctca actggcactt
     1381 tatgccaagg cagaggaagt gtatctccag gtcttggata actttccgga gagttccaac
     1441 tggagtctca ccgcactgaa ggctggacag ctcaaggaga acctttcgga gtttgctgcc
     1501 gcttcggaat tctatgcccg catcaagcgt cgtgatccca gtgaccctct gtgtaccgaa
     1561 tccgagtacc gtcgtctgga gatggaactc agaatggcgc agaccgaacc cggtgttgct
     1621 gctgaacgtc ggtttgcatc cgttttgcgc gatgcagtac aggctcaatc tgaataccca
     1681 gatgagatcc gtttctatgc ccagcaggga gttgcggcgt attcactgaa ggatttctcc
     1741 acggcagcgg acgcctttgt gaaatatctg gatcagtccg gtgacttgga gcggattccc
     1801 cgttgcactt tcctgctcgg cgatgcgctg ctgcagctgg aaagacatca ggaagccgca
     1861 gatatccttc agaagagaat cgagatggaa ggcgatgacg atgagcgtgc gcatgcgctg
     1921 tttctcttgg cggtgtgtca gaagaaattg aatgaattcg acaaggcgat tgtgactctg
     1981 aatgaactcc tcacaagtca cccgagcagt tcattcatac cggaggccag tgcggagaaa
     2041 tcacgcttgg aatggagttc caggaatgtt ggttatcttc agggaacgtc ccaggcaccg
     2101 tgacggaagc ctggaaatcc ttgccgagtc aggaaggcag tcttttccta tatgggatgt
     2161 tccgcgtgaa tggagtagag gtttgtggct cttaatagac tgaaaaataa gtacttatga
     2221 attcacgaga acctcggcat gttttctgct aagtactttg ggataagaag gagtactcga
     2281 acaaagcgat gtattttgaa cgcatcatac cgtggtcagc gagattgctt cgggaaggca
     2341 tgagtcagac ttcctttcga cacaacctga tcacgcacaa tatcgcgaac gtggacaccc
     2401 ccgggttcag acgaaaggat gtcacgtttg acgaagcact taagagggcg acgatggaac
     2461 tggtgtcgca agcccaaagt ggaagagcgt tttccagcgg tcagtcaact gctcgtcttt
     2521 cggtcttcga agacgacaga cctatgctga ggcaggacgg cagcaacgtt gacattgatg
     2581 aagaaatggc cttgatggcc atgaatgccg gcgactatat gaggtatgcg gaactgcatg
     2641 atcggcagat gcgtttgatt cgagcggcca tttcggagcg tatgccatga gtggttttct
     2701 catctcggca tcaggtttga cggcacagcg gacacgtatg aacgtgattg ctgagaacct
     2761 tgccaatgcg agcacgacgc atccaacacc ctatcggcgg aagattgtgc tctttgagga
     2821 gcgtcttgcc gaggcaatgg acagttctca atcgcgctcc ggtgcaggcg gtgttcgggt
     2881 taaaagcatc gtggaagaca acggccggaa tgcatttcgc tacctgtacg atccggatca
     2941 ccct
//
LOCUS       DAOQUC010000364         2939 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_39542_length_2939_cov_3.073856, whole genome shotgun sequence.
ACCESSION   DAOQUC010000364 DAOQUC010000000
VERSION     DAOQUC010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2939)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2939)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2939
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39542_length_2939_cov_3.073856"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(270..1121)
                     /locus_tag="PKH07_07355"
     CDS             complement(270..1121)
                     /locus_tag="PKH07_07355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34798.1"
                     /translation="MRCWSEEDDVFKKPVLLVTLMLSLPLLLFGVLSLRRNVTVEFQI
                     DPMHQIKSPLNSEEVDQLSREKGELLHELTSGVPPDASSEPTPSLLSKPDIVSDLDRL
                     AEKTDAWRYRSFYRIGEVEYGQLEDMASKQMIKVVEGGIHDEVIFTNLNESICTVVLG
                     EASRSLALDLVPEQPTETPLDADSPESLRFSDYRLKSEPTMRAIFSQYTPKDPNSPLR
                     TPPTSEEVETAAAAYYATVRPTVQAAEDMPELIQNPNPSEGLTELEEWVDTFRSVQLN
                     LRETPGH"
     gene            complement(1153..>1341)
                     /locus_tag="PKH07_07360"
     CDS             complement(1153..>1341)
                     /locus_tag="PKH07_07360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34799.1"
                     /translation="CSRLPSLIDWRMSGELKGRQHTSKQRLAVMERTCFIRFADGCQF
                     DRRKPALDLILGYVYIIE"
     assembly_gap    1342..1351
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1352..2210
                     /locus_tag="PKH07_07365"
     CDS             <1352..2210
                     /locus_tag="PKH07_07365"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNT34800.1"
                     /translation="LRTAIQRGADINGLDQQGMTPLYLSVVEGHEEIMRFLVESGADV
                     NGRTSEGFTPLIRAAESRAIQTMEYLISKGAQVGIAGNDGYIALHAVLIKADKTKQEV
                     LDMVELLLVHRADVNANSGPGKPLHYAVGGYGYPEIVQLLLSKGADVDAVGPDGTALS
                     LAAARDSLELVLLLLEHGADPNLEDYKGKRPLHNAARRGNEQIGKALLDHGADINARM
                     IGSETPLHEAMKYRHLSMMKFLLDNGADVNAETAQGTLLDEAEREQRKEMCSLLLKYG
                     ARRSNNPRK"
     gene            complement(2304..>2939)
                     /locus_tag="PKH07_07370"
     CDS             complement(2304..>2939)
                     /locus_tag="PKH07_07370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34801.1"
                     /translation="TDSGQGAHLRSALDNYFSATGNRSHIVGMWQRSLALTSNPNNGN
                     FDDLIYCVNSRLRWASLETYCSHQGFITGTDPDGNNIGQTDDYYLATRLTAPIRRWSL
                     VCTNSRLKGNLTPSKFGTYPGYSKSFYKFLNREFWFMANGWYDGARTQVSTNVKEILR
                     TGMDGYTWTPGEGLWQLGTGYTTRDGFFEKYIMWYSVGGNTNAHSDGVDAR"
BASE COUNT          713 a          738 c          792 g          686 t
ORIGIN      
        1 cgcgggttac tggcaacaaa tcgaggtcta tattcgcgat cattcggatg ccgtgttcag
       61 ccatggctac tgtccagact gccttgaaaa ggcgctgcag aagcagaaac cctcaggccc
      121 ttgagttcgg atgtgggagc actcgctgag gcagggactt ggaatattcg tcctggatca
      181 gcctgtcccg ttttcgcagg atatttgaac ccggacactg aaacgtggta tgccgtccag
      241 acgccgttct gtacaaacat tgtccaagct cagtgtccgg gtgtttcgcg cagattcagc
      301 tgaacggaac gaaaagtgtc aacccattcc tccagttctg tcagcccttc tgagggattc
      361 gggttctgta tgagttcagg catgtcttcc gctgcttgta ctgtcggcct gaccgtggcg
      421 taatacgcgg cagcagcagt ctcgacttct tctgacgtgg gaggagttct gagggggctg
      481 tttggatctt tcggcgtgta ttgggagaag attgcgcgca tggtcggttc gctcttcagg
      541 cggtagtcgg aaaagcgcag agattccgga gagtctgcgt ccaatggtgt ttctgttggc
      601 tgctcaggaa caaggtcaag agccagagat ctcgaggcct ctcccagaac gacagtacag
      661 atggattcgt tcaggtttgt gaagataacc tcgtcgtgta tcccaccttc cacgaccttg
      721 atcatctgct ttgaagccat gtcttccagt tggccatatt ctacctcacc gatccgataa
      781 aatgagcggt agcgccatgc atccgttttt tcggccaatc gatcaagatc agaaacaatg
      841 tcaggtttgg aaagcaggct tggtgtcggt tccgaagatg cgtccggtgg gacgccagat
      901 gtcaactcat gcagcaattc acctttctcg cgcgagagtt gatccacttc ttcactgttc
      961 aaagggctct tgatctgatg catgggatct atctgaaatt cgacggtcac atttctgcga
     1021 agcgagagga ctccaaaaag cagcaacggg agagagagca tcaaagtgac caagagaaca
     1081 ggcttcttga acacgtcatc ttcctctgac caacaacgca tctcaatgtg tgcacaaact
     1141 tattgcacat gttcattcaa taatgtaaac atatcctaga atgagatcaa gtgctggttt
     1201 tcggcggtca aactgacatc catcggcgaa tctgatgaaa caagtcctct ccatcacggc
     1261 aagtctctgt ttgctggtat gctgtcgccc tttgagttct ccagacatcc gccagtcaat
     1321 caaggagggc aacctggagc annnnnnnnn nacttcgtac cgcgattcag cggggagctg
     1381 atatcaacgg acttgaccaa caaggaatga caccgctcta tctctctgtt gtcgaaggac
     1441 atgaagagat catgaggttt ctggttgaaa gcggtgctga cgtcaacggc aggacgtctg
     1501 aaggattcac cccgctgatt cgtgcggcgg agagtcgagc tatccaaaca atggagtacc
     1561 tcatatcaaa aggcgctcaa gtcggtattg ctggcaatga cggatacatc gcacttcatg
     1621 ccgtgctcat caaggcggac aagacaaaac aggaagtcct ggacatggtg gagttgttac
     1681 ttgtccacag agcagacgtc aatgccaaca gcggacctgg aaaacccctt cactatgcgg
     1741 tcggcggata cggttatcct gaaattgtgc agctgctcct ttcgaagggt gctgatgttg
     1801 atgcggttgg gcctgacggg acagccttga gccttgccgc tgcccgagac agtctcgagc
     1861 ttgttctcct gctgctagag catggagcag accctaatct ggaagactac aagggcaagc
     1921 gcccgctaca caacgccgcg cgaaggggca atgagcaaat cggcaaggcc ttgctggatc
     1981 atggtgccga tatcaatgcc agaatgatcg gcagtgagac gccgctccat gaggcgatga
     2041 agtaccgcca tctgtccatg atgaaattcc tcttggacaa cggtgcggat gtaaatgcgg
     2101 agacagcaca aggaacgcta ttggatgaag ctgagcggga gcagagaaaa gagatgtgca
     2161 gtcttcttct gaaatacggc gcaagaagat cgaacaatcc acgaaaatga tctgttttcg
     2221 gaatgaaaac agagcccgag cgcgatcaga acgatcgggc tcgggctccg ttttgtgaag
     2281 tccaaaacga gaaacgcagt tctctagcga gcgtctacac cgtcagaatg agcgttcgtg
     2341 ttgcccccaa cgctgtacca catgatgtac ttctcaaaga agccatcgcg agtcgtgtag
     2401 cccgttccaa gttgccaaag accctcaccg ggtgtccaag tataaccatc cataccggtt
     2461 ctgagaatct ccttgacgtt ggtgctgacc tgcgttcgag ccccgtcata ccaaccgttt
     2521 gccatgaacc agaactcacg gttgaggaat ttgtagaacg acttgctata gccgggatag
     2581 gtgccgaact tcgagggagt cagattgccc ttcaatctcg aatttgtgca taccaacgac
     2641 caccttcgaa tcggagccgt caaacgtgtt gccaagtagt aatcatcagt ctgtccaatg
     2701 ttgttcccat cgggatcggt gcccgtgatg aaaccttggt gcgaacagta ggtctcaagg
     2761 ctcgcccagc ggagtcttga gttgacgcag tagatcagat cgtcgaagtt gccgttgttg
     2821 gggttggatg tcagcgcaag gcttctctgc cacattccaa caatgtgaga gcgattgccg
     2881 gtagcgctga agtagttgtc caacgccgat cgaagatgcg ccccttggcc ggaatctgt
//
LOCUS       DAOQUC010000365         2938 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_39566_length_2938_cov_2.415539, whole genome shotgun sequence.
ACCESSION   DAOQUC010000365 DAOQUC010000000
VERSION     DAOQUC010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2938)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2938)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2938
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39566_length_2938_cov_2.415539"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="PKH07_07375"
     CDS             complement(<1..295)
                     /locus_tag="PKH07_07375"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNT34802.1"
                     /translation="MTLSSAEVRKQSLSVFFPCHNEADNIGALVDKTVAVLRKLVDDF
                     EVIIVNDGSKDKTREVADALAAKYPEVRAVHHEVNKGYGGAVWTGLRSATKQLV"
     gene            complement(387..1382)
                     /locus_tag="PKH07_07380"
     CDS             complement(387..1382)
                     /locus_tag="PKH07_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNT34803.1"
                     /translation="MLRVCVIGMGHIGNLHASIYQEDPLVDLVGVCDLIEQRARTAGE
                     KFGVPWFLSAEKMLSALAPDICSIATGGYEYSSDHYEPTIQALEAGCHVLCEKPICNQ
                     IQHAEEMVRVARERKRCFGVDFNHRFTPAARIAKQWMDEGRLGSLLFVNMGLWIGRPK
                     DEDSPYYHIKALNPHSVDMIRHYCGSIVKIHCFAMKAPGRTMWSTASFNMLLANGMVG
                     HLTSSYDIARGHPMERCEVAGLKGRFVLQDMWREVTLYPADSDVKSVYTNPVFGGYRD
                     FGDTFKERLHCFVKQVADRVAPEDIDGSGMDGLRAQKVIAAGIRSLETGLAVDVD"
     gene            complement(1385..2116)
                     /locus_tag="PKH07_07385"
     CDS             complement(1385..2116)
                     /locus_tag="PKH07_07385"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HNT34804.1"
                     /translation="MLITIIGRGHSGTRAMSHTLSASGVYMGAQLNGSGDLIPPGDMY
                     EACRVMGKYLVHKGGLEWDFSKVLNDPIDPEFEGLIRNYLSSVLNSDAERKGWKIPET
                     TLVYPWIVRMFPDAYYIHWVRDPRDSIIGAHLTDNLADFGVPYDFTDDLRLRRAISWK
                     YQREIVKATPVPKRVIHVRFEDFVLKQQETLRRLEQFLGFPLETIPVSRDPIGRWKTD
                     EGRHIFDFLLQDMDELGYEVPAEVR"
     gene            complement(2237..>2938)
                     /locus_tag="PKH07_07390"
     CDS             complement(2237..>2938)
                     /locus_tag="PKH07_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNT34805.1"
                     /translation="IRALGLEEDTLLVFDADHGETLYDHDCFFDHHSIYDPTLHIPLF
                     FHLPGKVPAQRFSDICQMKDITPTILDILGMKTKIKFDGRSLQPLMQGKKRVPEPEMY
                     LTECTWMRKHGWRTPEWKLIHALEPDFHFKPEVELYNLITDPEENQNLAKKETKVVQM
                     LEERMQAHIAKREKQTGRTNPMYTNLNWHGHGCGPFKTSQQAYDTMHIGSPKTAEKIQ
                     AQMRELRTQRGAKKL"
BASE COUNT          682 a          845 c          714 g          697 t
ORIGIN      
        1 agaccagttg ctttgtggcg gaacgaagcc cagtccagac cgcaccccca taacctttgt
       61 tgacctcatg gtgtacggct ctaacctcag gatactttgc cgccaaagcg tcagcgactt
      121 ccctggtctt gtccttggag ccgtcgttga cgatgatgac ctcgaaatcg tcaacgagtt
      181 ttcgcagaac ggcgacagtt ttgtccacca acgcgccgat attgtcggct tcattgtgac
      241 acgggaaaaa gaccgataaa ctctgtttgc gaacttcagc gctgctcaaa gtcatatcaa
      301 aacagccccc tcaatctgtg atcttgtctt cattatggac aaggcagcag aactgtcaag
      361 agcgaagcac aatgtgcttc ttgcgttcag tccacatcca ccgcaagacc agtttcgagt
      421 gaccggattc ctgcagcgat gactttctgt gccctcagac cgtccattcc tgagccgtca
      481 atatcttctg gagcgacgcg atcagcaacc tgtttcacga agcaatgcag tcgttcctta
      541 aacgtgtctc cgaagtcacg gtatccgccg aagacggggt tggtgtaaac cgacttcaca
      601 tcgctgtcag ccggatagag cgtcacttca cgccacatgt cctgcaggac gaaacgcccc
      661 ttaagaccag ccacttcgca gcgctccatc ggatgcccac gagcaatgtc atagctgctt
      721 gtcaggtgtc ccaccatacc atttgcgaga agcatgttga atgatgcagt tgaccacatg
      781 gtgcgtccag gagccttcat cgcgaaacag tgaatcttca caatactgcc gcaatagtgg
      841 cgaatcatat caaccgagtg agggttcaat gccttgatgt gatagtaggg agagtcctca
      901 tcctttggtc tgccaatcca cagtcccata ttgacgaaaa ggagagaacc cagacgtcct
      961 tcatccatcc actgcttggc aatcctcgca gcgggtgtga accgatggtt gaagtccacc
     1021 ccaaagcacc gtttgcgctc acgggcaacg cgcaccatct cttccgcgtg ctgaatctgg
     1081 ttgcagatgg gcttctcgca cagaacgtga cagcccgctt ccaaggcttg gatcgtcggc
     1141 tcgtagtggt cgctgctgta ttcgtagcca cccgtcgcaa tgctgcatat atctggagcc
     1201 aaagcactca gcatcttctc cgcagacaga aaccacggaa ccccaaactt ctctcccgct
     1261 gttcgcgctc gctgttcgat gagatcacag acaccaacca gatccaccag aggatcttcc
     1321 tgatagatac tggcatgaag atttccaata tgtcccatgc caatgacgca aacacgcagc
     1381 atggctatcg cacctctgct ggaacttcgt agcccagttc atccatatcc tgaagcagga
     1441 aatcaaatat gtgccgacct tcgtccgtct tccagcgtcc aataggatca cgacttacag
     1501 gaatagtctc caatgggaag ccgaggaatt gctccaggcg tcggagtgtc tcctgctgct
     1561 tgagcacaaa atcctcaaat cgaacatgga tcacccgctt gggcactggt gtcgccttga
     1621 cgatctctcg ctgatacttc cagctgatcg cccgacgcag cctgagatca tcggtgaagt
     1681 catagggcac tccaaagtca gccaggttgt ccgtcaggtg ggcgccaatg atcgagtcac
     1741 gcgggtcgcg cacccagtga atgtagtaag catcaggaaa catccgaacg atccagggat
     1801 acaccaaggt cgtctcggga atcttccagc ctttgcgctc tgcgtctgaa ttgaggacag
     1861 aagaaaggta gttgcggatg agtccctcga attccggatc aatgggatca ttaagcacct
     1921 tggaaaaatc ccattccaaa ccgcctttat gcaccagata cttgcccatc actcgacagg
     1981 cttcatacat gtctcctgga ggaatcaggt cacccgaacc gttcaactgc gctcccatgt
     2041 aaaccccgct ggcactcagc gtgtgcgaca tggcacgcgt tcccgaatga cctcttccga
     2101 taattgtgat caacattcaa gtttctcctt ggaaactgat ctcctcgtga cacagagtcc
     2161 gagagagtcg tgacatctca tgagcaagcg actgaaggag tgggcatagc ccacacgccc
     2221 gcaggtgcta acctgctcag agtttctttg ctcctcgttg tgttctgagt tccctcatct
     2281 gcgcctgaat cttctccgcc gtcttgggag agccaatatg catcgtgtca tacgcctgct
     2341 gggatgtctt aaacggccca cagccatgac catgccagtt cagattcgtg tacatggggt
     2401 tggtgcgtcc cgtctgcttc tcgcgcttgg cgatgtgtgc ctgcatgcgt tcttcgagca
     2461 tctgaaccac tttggtttcc ttcttcgcca agttctggtt ctcctccgga tcggtgataa
     2521 ggttatacag ctccacttcc ggtttgaagt ggaagtccgg ctccagcgca tggatcaatt
     2581 tccactcggg ggttctccaa ccgtgctttc gcatccaggt gcattcggtc agatacatct
     2641 ccggctccgg aacgcgcttc ttgccctgca tcaacggctg aaggcttcga ccatcaaact
     2701 tgatctttgt cttcatcccg agaatgtcaa gaatggtcgg tgtgatgtct ttcatctgac
     2761 agatgtctga aaaacgctgc gccggaacct ttccaggaag atggaagaac aacgggatat
     2821 gcagagtcgg gtcatagatg ctgtgatgat cgaagaagca gtcgtggtca tagagcgtct
     2881 cgccgtgatc tgcatcgaac accagaagcg tgtcttcctc aagcccaagc gctcgaat
//
LOCUS       DAOQUC010000366         2931 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_39720_length_2931_cov_3.765647, whole genome shotgun sequence.
ACCESSION   DAOQUC010000366 DAOQUC010000000
VERSION     DAOQUC010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2931)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2931)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2931
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39720_length_2931_cov_3.765647"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..473)
                     /locus_tag="PKH07_07395"
     CDS             complement(<1..473)
                     /locus_tag="PKH07_07395"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNT34806.1"
                     /translation="MSKGGRRGRVLNALLCWSDLLRLPDRPLSFPLTIWVENTTACNL
                     RCEMCYKSASTSVCPSNVHLPPEFMERLLPEAQSACFSLLLGYGEPILSPNFFPWLER
                     LIGIGCTTTTSLNGLILTSERARKIVESGVAQVSFSLDATTAETYSQIRKGGDWD"
     gene            complement(487..1215)
                     /locus_tag="PKH07_07400"
     CDS             complement(487..1215)
                     /locus_tag="PKH07_07400"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010322377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HNT34807.1"
                     /translation="MKVRLGVNIDHVATLRQQRGTLYPDPVAAAVLAQLGGADCITVH
                     LREDRRHIQDRDVRLLREMLEISLNLEMAATDEMVQIASQVKPDQVTLVPEKREERTT
                     EGGLDVVSQKEHLKKQIARLHDAGIPVSLFVEPERAALDGSAAVGAGIVELHTGRYAD
                     AISSEAQHQELQSLIQASDYSHQIGLTVNAGHGLHYRNTRPVAEIKGMNELNIGHSII
                     CHAVFVGLERAVREMRDILDSVSD"
     gene            complement(1252..1947)
                     /locus_tag="PKH07_07405"
     CDS             complement(1252..1947)
                     /locus_tag="PKH07_07405"
                     /inference="COORDINATES: protein motif:HMM:NF019561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HNT34808.1"
                     /translation="MAQGIFSFRIRNLGLKVLALVLSLLLWYLATDSRLRSKVINRVP
                     LQFQSENLPPNMYHEISGERFSSFKVLGPSEDIASLTADNFMVRIPLQSVSVGPNTIP
                     IRREMIAKINLQDRVKKRVVVLPGSIVPDQITIDIAYYQREVPIEVVTSGDPAPGHHV
                     KETQITPETVLVTGSPDELDKITEIPTDTLDITNIESNVTVDVMLNFKGLSVQPVSEA
                     SRQVRVNFVVQKK"
     gene            complement(1960..2715)
                     /gene="cdaA"
                     /locus_tag="PKH07_07410"
     CDS             complement(1960..2715)
                     /gene="cdaA"
                     /locus_tag="PKH07_07410"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HNT34809.1"
                     /translation="MWLHIFLSDPLRNILDVSVLWVLIYYVVKVLRGTRAMQVLQGLL
                     LLLGLSVFARWASLYTINWVLDNFWPVLLISLVILFHPEIRRLLAGIGRRSLFRTLLR
                     DQSETVEIVVKAASLLSKKHIGALVAFEREGSLQRFVDTGVQIDGMVNADLLTTIFTP
                     HTPLHDGAVVIQGDRISAAACLFPLTERELADKSLGTRHRAAIGLTEEVDAIVVVISE
                     ETGTISVAIGGRLTRDLDPAQLRHILTRLFHRR"
BASE COUNT          677 a          817 c          784 g          653 t
ORIGIN      
        1 ttgtcccagt cgcccccctt tcggatttgg gagtaagttt cagctgtggt cgcgtccaga
       61 gagaaactca cctgtgcgac accggactca acaatctttc gcgcccgctc cgaggtgaga
      121 atcagaccat tgagacttgt tgtcgtcgtg catccgatgc caatcagccg ctccaaccag
      181 gggaagaagt tcgggctgag aatcggctct ccgtatccaa gcaacaggga gaaacatgcc
      241 gactgcgctt ccggaaggag acgctccata aactctgggg gaagatgcac gttggaggga
      301 cagacactgg ttgaggcgct cttgtagcac atctcgcaac gcagattgca cgccgttgta
      361 ttttcgaccc agatggtcag gggaaacgaa agaggccgat cgggaaggcg caggagatca
      421 gaccagcaca acaacgcatt gagcactcga ccgcggcgtc cgccttttga caaggcacat
      481 cactccttaa tctgaaacgc tgtcaagtat atcccgcatc tcccgaacgg cgcgttccag
      541 accgacaaag accgcatggc agatgatgct gtggccgatg ttcagttcat tcattccctt
      601 aatttcggca acgggtctcg tgtttcgata gtgaagaccg tggcctgcgt tgaccgtcag
      661 gccaatttga tgcgagtaat ctgaggcctg aatcagactc tgcagctcct ggtgctgtgc
      721 ttcagaggaa atcgcatctg cgtatcgtcc ggtgtgcagt tccacaatcc cggcacctac
      781 ggctgcgctg ccgtccagcg cggcgcgctc cggctccaca aacaacgaga cagggatacc
      841 ggcatcatga agtcgggcga tctgtttctt caggtgttcc ttttgcgaga caacgtccag
      901 cccaccttct gtggtgcgtt cttcccgttt ctcgggaacg agggtcacct gatcgggctt
      961 gacttgagag gcgatctgaa ccatttcatc agtggctgcc atctccaaat tgagggagat
     1021 ctcaagcatc tcacgcaaca gacgcacgtc tctgtcctga atgtgccgtc ggtcttcacg
     1081 cagatgaact gtaatacaat ccgccccacc cagctgcgcc agaaccgctg cggcaactgg
     1141 atcgggatac agagttcccc gctgttgccg cagagtggca acatggtcaa tgtttactcc
     1201 caaacgtacc ttcatgggga ttatctcctc tctgaacact ctcaagacag atcatttttt
     1261 ctgtacaaca aagttcacac gaacctgccg actggcttcg gaaacgggct gtaccgaaag
     1321 ccctttgaag ttcagcatga cgtccacggt aacgttgctt tctatgttcg tgatatccaa
     1381 tgtatcggtg ggaatttcgg tgattttgtc cagttcgtcg ggggaacccg tgacgagaac
     1441 ggtctccggc gtgatctgcg tttccttgac atgatgcccg ggtgccggat ctcccgatgt
     1501 caccacctcg atggggacct ctctctggta gtaagcgatg tcaatggtga tctggtcagg
     1561 cacaatggag cctggcaaca caacgacgcg tttcttgacg cgatcctgca ggttgatctt
     1621 ggcgatcatc tctcgacgga tgggaatggt gttgggaccg acagaaacac tctgaagtgg
     1681 aatcctgacc ataaaattat cagcagtcag cgaagcaatg tcttcgctgg ggcccagaac
     1741 cttgaatgag ctgaaacgct ccccggagat ttcatgatac atgttcgggg ggagattctc
     1801 gctctggaac tgaagtggga cgcggttgat gaccttgctt cttagtcggg agtccgtcgc
     1861 gagataccac agcaaaaggg aaagcaccag cgccaggacc ttcagcccca ggttgcggat
     1921 gcgaaaagag aaaatccctt gtgccatgtt tgcgtccttc tatcgtctgt ggaagagccg
     1981 ggtcagaatg tgtcgcagct gcgccggatc gagatcgcga gtcagccgtc ccccgatcgc
     2041 tacggagatt gttcccgttt cttctgagat caccacgaca attgcatcca cttcctctgt
     2101 cagtccgatg gcagcccgat ggcgggttcc caggctcttg tcggccagct cgcgctcggt
     2161 caggggaaag agacatgccg ctgcgctgat tcgatcccct tgaatcacca ccgctccgtc
     2221 gtgcaacggc gtgtgcggag taaaaatggt cgtcagcaaa tccgcattca ccatgccatc
     2281 aatttggact ccggtgtcaa caaaacgctg aagactcccc tcacgctcaa aagcaaccaa
     2341 agcgccaatg tgcttcttcg acagaaggga tgctgctttc acaacaatct cgacggtctc
     2401 agactggtct ctcagcagcg ttctgaacag agagcgtcgc ccaattcccg ccagcaggcg
     2461 ccgaatctcc ggatggaaca ggatcaccaa agagatcaac aagaccggcc agaagttatc
     2521 caatacccag ttgatggtat agaggcttgc ccagcgcgca aaaacggaaa gaccaagcag
     2581 gagcaacaga ccttggagaa cctgcatggc acgggtcccg cgcaacactt tgactacgta
     2641 gtagatcaga acccacagaa ccgaaacatc aagaatgttc cgaaggggat ccgacagaaa
     2701 tatgtgcagc cacataagcg ttagccgttc atgccttgcc cgcgtagacg gtctgtctca
     2761 gttttcgttt aggcagagtt cctgagccga cggcgaaccg attgcctcta ggcgttgccg
     2821 agcctctgat tcagttttct tctacgctca taggcatact ttcgagcgtt ggtcttcaag
     2881 cggatgttgg tgaatggatc acgcaccgca tcggtattgt cggaatgtcg c
//
LOCUS       DAOQUC010000367         2926 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_39834_length_2926_cov_2.720307, whole genome shotgun sequence.
ACCESSION   DAOQUC010000367 DAOQUC010000000
VERSION     DAOQUC010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2926)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2926)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2926
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39834_length_2926_cov_2.720307"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1081
                     /locus_tag="PKH07_07415"
     CDS             <1..1081
                     /locus_tag="PKH07_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="homocysteine biosynthesis protein"
                     /protein_id="HNT34810.1"
                     /translation="VVTTGTFGPMCSSGAYINIGHAKPRIKLGGGHVRLNDVPAYAGF
                     AAVDVYIGATALPDEDPRNRNHPGEFRYGGGHVIEELVSGKDIRLTATAYGTDCYPRR
                     KLETYINIKDVNEAVLFNPRNSYQNYNVAVNLSDRPIYTYMGILKPKLGNANYCSAGQ
                     LSPLLNDPNYRTLGIGTRIFLGGGEGYVVWHGTQHNPGVKRGENGVPRMPAGTVAVIG
                     DLKQMESKWLKGVSFRGYGTSLSVGIGLPIPVLDEDVCRSAAVNDEDIYATIVDYSKS
                     YPYVEPGNLGEVSYAQLKSGSITLEGKQIPTTPLSSYVKAKEIANTLKERIQRGQFLL
                     TEPVAPIPKADSGYAFRPLAERPIE"
     gene            1239..1961
                     /locus_tag="PKH07_07420"
     CDS             1239..1961
                     /locus_tag="PKH07_07420"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HNT34811.1"
                     /translation="MEIMGHRGAAGVAPENTMPAFKVSADLGIPWVELDIRLTKDGDL
                     VLLHDKSLERTTDGTGEVGQLTVAEIQKLDAGSWFSADFAGVRVPTLVQVLETFQERI
                     QFNIEIKDEDDRLEEQIARLFEILDRHQLRHRVILSSFKEKILGAVREEDERVQLAII
                     ANQSADQMKRIATELSCQMMNPGNKILSEPLIKWAHERGKRITGWMANEKEILHLLNQ
                     WNVDYCCTDFPGMAQEYLEELP"
     gene            1958..>2926
                     /locus_tag="PKH07_07425"
     CDS             1958..>2926
                     /locus_tag="PKH07_07425"
                     /inference="COORDINATES: protein motif:HMM:NF013468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HNT34812.1"
                     /translation="MTDSAPTLSITIQSVKRIRLDIRGVVQGVGFRPFVTRLARDLSL
                     SGNVRNCGSGVIIEVQGMSEAVEQFQSNLVSNAPEHARIERVEKMEVEVEAAEGFSIL
                     DSLDQHAQRVLIPPDVGCCDDCLTEVFDEGNRRYRYAFTSCVNCGPRFTLIEGLPYDR
                     TKTTMARFEMCSSCRKEFEDVDNRRYHVQPNACSVCGPRVTFLDSNGQPIECADPIAE
                     AARAIRAGRIVAVKGLGGYHLACDATNPHAVSLLRHRKRRPNRPFAMMFPDVQSVRRL
                     CELSSEEERELTGSRRPIVLLAVKKQIGASTLTQRRPRVPVEEVAPG"
BASE COUNT          697 a          688 c          888 g          653 t
ORIGIN      
        1 tgtagtgacg acagggacat ttggtccaat gtgttcttcg ggagcctaca tcaacattgg
       61 tcacgcgaag ccgcggatca aacttggagg aggccatgtt cggctgaatg atgtgccggc
      121 gtatgcggga tttgcggcgg tggacgtgta tataggagcg acagcgctgc cggacgagga
      181 tccgcggaac aggaatcatc ctggggagtt tcggtacggt gggggtcatg ttatcgagga
      241 gttggtgtct ggtaaggaca ttcgactaac ggcaacggcg tacggcacgg actgttatcc
      301 tcggaggaag ttggagacgt acatcaacat caaggatgtc aacgaagcgg tcttgttcaa
      361 cccgaggaac tcgtatcaga actacaatgt tgcggtgaat ctttcggatc ggccaattta
      421 cacatacatg ggaattctga agccgaagtt gggcaacgcg aactattgta gcgcaggcca
      481 gttgtcaccg ttgctgaacg atccgaacta ccgaaccttg ggcattggaa ccaggatttt
      541 tctgggcgga ggagaaggct acgttgtgtg gcacgggaca cagcataatc ctggagtgaa
      601 gcgaggagag aacggagtcc cgaggatgcc agcggggacg gtggcggtga taggcgacct
      661 gaagcagatg gagtcgaagt ggttgaaggg agtatcgttt cgagggtatg ggacatcgtt
      721 atcagttgga attggactcc cgatacctgt tctggatgag gacgtttgtc ggagtgcagc
      781 cgtgaacgat gaggatatct acgcaacgat tgttgattac agcaagagtt atccttatgt
      841 tgagcctggg aatctgggag aggtgagtta cgcccagttg aagtcgggtt cgatcacgct
      901 tgagggcaag cagattccta caactcctct ttcaagttat gtgaaggcga aagagatcgc
      961 caacactttg aaggagagaa tccaacgcgg ccagtttctt ctgacggaac ccgtcgcacc
     1021 gatcccgaaa gcggattccg gttatgcgtt ccgaccgctg gcggaaagac cgatcgagta
     1081 agcggcaagg tcgcaccaca gatcaagcga ctgagtatgg gagccagacc tgttctggct
     1141 cccaactctt ctgttcggaa agcaggtttc cggcgtccat tcttgacaaa cccatggctt
     1201 tcgataatgc ttttctccat tgtgaggagg gtcagcaaat ggagataatg ggtcatcggg
     1261 gagcagcagg agttgctccc gagaacacga tgccggcatt caaagtcagc gccgatttgg
     1321 ggattccttg ggtcgaactg gatattcgtc tcaccaagga cggtgatctg gttcttttgc
     1381 acgacaagtc cctggaacgc acgaccgatg gcaccggaga agtgggtcag ttgaccgtgg
     1441 ctgagattca gaagttggac gccggcagtt ggttctcggc ggactttgcc ggtgttcgag
     1501 tccccacact cgtgcaggtt ctggagacat ttcaggaacg gattcagttt aatatcgaga
     1561 tcaaggatga agatgaccgt ttggaggaac agattgcgag actgtttgag attctcgacc
     1621 gccaccaact gcgccatcga gtaatcctgt cgagtttcaa ggagaagata ctaggcgctg
     1681 tccgcgagga agatgaacga gtgcagctgg cgatcatcgc caaccaaagc gctgaccaga
     1741 tgaagcgaat cgcgaccgag ctcagctgcc aaatgatgaa cccaggcaac aagattctat
     1801 cggaaccatt gatcaagtgg gctcatgagc gtggcaagcg gatcaccggc tggatggcca
     1861 atgagaagga gattctccac cttttgaacc agtggaatgt tgactattgc tgcaccgact
     1921 ttccggggat ggctcaggag tatcttgagg agctgccttg accgattctg ctcccactct
     1981 cagtatcacg attcagagcg tcaagcgtat tcggcttgac ataagaggcg tggttcaagg
     2041 agtcggcttt cgaccctttg tcactcgcct agccagggat ttgagccttt ccgggaatgt
     2101 gagaaactgt gggagcgggg tgattatcga agttcaaggc atgtctgaag cggtggagca
     2161 gttccagagt aatctagtct caaacgcccc agagcatgcc cgaattgagc gagttgagaa
     2221 gatggaagtg gaagtagagg ccgctgaagg tttctcgata ttggacagcc tcgaccaaca
     2281 cgctcagaga gtcctgattc ctcccgatgt tggctgctgt gacgactgtc ttaccgaagt
     2341 tttcgacgag gggaatcgca ggtaccgata tgcattcacg agttgtgtga attgtggacc
     2401 gagattcaca ctgatcgagg gacttcccta tgatcgaacg aaaacaacga tggcacggtt
     2461 tgaaatgtgt tcgagctgcc gaaaggagtt cgaggatgtg gacaaccgac gttaccatgt
     2521 tcagcccaac gcctgctcgg tctgcggccc gcgtgtcact tttctcgact cgaacgggca
     2581 accaattgaa tgcgccgatc ctatcgctga agccgcaagg gcaatccgcg caggccgcat
     2641 agttgcggtg aaagggcttg gtggctatca ccttgcgtgc gacgccacca acccacatgc
     2701 ggtctcgctg ctcaggcatc gcaagcgcag gccaaacaga ccatttgcca tgatgtttcc
     2761 tgatgtgcag tccgtccgac ggttgtgtga actgtcgtcg gaagaggaaa gagaattgac
     2821 tggttctcgt cgtcccatcg ttctgttggc cgtgaaaaag cagatcggcg caagcacgct
     2881 gacacagcga cgacctcgtg ttcctgtcga ggaggtcgct cccggg
//
LOCUS       DAOQUC010000368         2925 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_39869_length_2925_cov_2.672474, whole genome shotgun sequence.
ACCESSION   DAOQUC010000368 DAOQUC010000000
VERSION     DAOQUC010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2925
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39869_length_2925_cov_2.672474"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1723
                     /locus_tag="PKH07_07430"
     CDS             <1..1723
                     /locus_tag="PKH07_07430"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HNT34813.1"
                     /translation="AIFGVPFNASDAELGVQYVELYYRKDGQGAYVKYGTTFTNSPIS
                     FDSGTTGGDGIYEFYTVATDNSSNSESAPSVADAMTRVFGSFSGSRVYVDCNASGSET
                     GLSWTDSFKTIDVAAFVANAFAVASVWVADGTYSESVTLLSGVSLYGGFAGTETDLSQ
                     RNVVSNETTIDASLVNGGSPAMHAVLIRNVTQARLDGFRITGAGALGSEPGGGVLCIN
                     ADDTNAVANCTITANQCGSGAGVFAQHSALRFESCLISQNNSAGYGPGAGVYLDYCEA
                     VVLNECTISENTGSDGSGIFAYMSNVSIEDCVIRDNVATSYSSLGGGIHFRYGESLLV
                     TNTLIEGNFAGWCGGGAYIYEASPVFTNVIFNANVSQWGGAAVSSYYAEPEFVNCNIT
                     NNVGTACGGFWAYYGSKPSFTNTIFQGNNRYAINAYGLSAAPPVTNCLFYNNADGDYF
                     IDGYSPTPFIGAAQINAAVPGASGNIDGDAQFVNSSVGDFRLSNLSPCVDSGTDLDAP
                     LFDFAGDLRPHDVPGRGADSTGKEFDIGAFENQDTGVYTPTNTPTQTATPLPTGVTGV
                     HVWEEYR"
     gene            1914..>2925
                     /locus_tag="PKH07_07435"
     CDS             1914..>2925
                     /locus_tag="PKH07_07435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34814.1"
                     /translation="MSDSSGFVDHGIPVKVSSTFSSAVGMDANGKAHFYQARGQSPTP
                     FFILDICLDTHETVQANTQVPGACFPWGMCVASNGKVYVGTADTGHLFEYDPLSKQLV
                     DLGRPGEESYIWTLAEGTDKKIYGGTYGRCKLVRYDPATGQSDDLGRMDPTEQYLRSV
                     VADSDGYVYAGIGSARAGLIAYHIESATWSQLIPEDQRVPGWGAVHIGEDGQCYATSP
                     AGNFHLTGGIAIPVETPAKKSGYLLPDGRSLSKHDAEKNYVIVKSATDSQEIRIDYEI
                     ENAGTRIFVVDKAPDGKVYGSSFLPLQLFVYDPETGQSQNLGKPTVSAGEIYSFTIFK
                     NQL"
BASE COUNT          670 a          725 c          792 g          738 t
ORIGIN      
        1 cgcgattttt ggtgttccgt tcaatgcttc agatgcggaa ttaggtgtgc agtacgtcga
       61 actgtactac cgcaaggacg ggcaaggtgc ctacgtcaag tacggcacaa cctttaccaa
      121 cagtcccatc tcttttgaca gcgggacgac cggtggtgat gggatttatg aattctacac
      181 cgttgctaca gacaattcga gtaattcgga gtctgcacca tctgttgcag atgccatgac
      241 acgagtcttt ggcagcttct caggatccag agtttatgtg gactgcaacg cctcgggcag
      301 tgaaaccggc ctgagttgga ctgattcctt caagacaatt gatgtggcgg catttgttgc
      361 caacgcattt gctgtcgcct ctgtctgggt tgcagacggt acctattctg aatctgtcac
      421 gctgctgagc ggcgtctctc tttacggtgg gtttgccggc actgagactg atctctcaca
      481 gcgcaatgtc gtgtcaaatg agacaacgat cgatgcttca ttagtaaacg gcggatcgcc
      541 agcgatgcat gcagttctca tcaggaacgt gacccaggca aggctcgacg gctttcggat
      601 aacgggtgca ggtgctctgg gctccgagcc aggtggtggt gtcctgtgta tcaacgcgga
      661 tgacaccaat gcggttgcca actgtacgat caccgcaaat caatgtggat cgggagcggg
      721 cgttttcgca cagcacagtg cgctgaggtt cgaatcttgt ctgatttctc agaataacag
      781 tgctggttat ggacccggtg caggagtcta tctcgactat tgtgaagccg ttgtccttaa
      841 cgagtgcaca attagtgaaa acactggtag cgatggatcg gggatttttg cctacatgag
      901 caatgtgtcc atcgaagact gtgtcatcag ggacaatgtt gcgacatcgt actcgtcatt
      961 gggtggcggg attcatttcc gttacggtga atcgttgttg gttaccaata ccctgatcga
     1021 agggaacttt gctggatggt gcggtggtgg agcgtatatc tatgaagcgt ctcccgtttt
     1081 caccaatgtc atcttcaatg caaatgtctc acagtggggt ggcgctgcgg tatcttccta
     1141 ctatgccgaa cccgagtttg tgaactgcaa tatcaccaac aacgtgggaa ccgcctgcgg
     1201 tggtttctgg gcgtactacg gttccaaacc gtctttcaca aacacgatct ttcagggcaa
     1261 caacagatat gcgatcaatg cctatggact atcggccgct cccccggtaa cgaactgcct
     1321 cttctacaac aacgcggacg gtgactattt cattgacgga tactcaccga cgcccttcat
     1381 cggtgccgca cagatcaacg ctgctgttcc cggagcatct gggaatattg acggtgatgc
     1441 gcagttcgtc aactcttccg ttggagattt ccgcctctcc aatctgtcgc cctgcgtgga
     1501 ctcgggaaca gatttggacg ctccgctttt tgactttgct ggcgatctca gacctcatga
     1561 cgttccaggc aggggtgctg acagtacggg taaggagttc gacatcggcg cgtttgagaa
     1621 tcaggatacc ggcgtttaca cgcccacgaa cacaccaact caaacagcga ctccgctgcc
     1681 aactggagtg accggcgttc atgtttggga agagtacaga tagtccgccc ctgatccaag
     1741 gggactgacg atgggaaagt ctcgaacagg gaaaagcgag attaaaccct ccgattgttg
     1801 acatgagaga cgggtttgtt actatcgcca atatgaaacg tctatcgctt cttcagctca
     1861 tgttggtggt tctcgtgtct tttccgtcat tctgtgttgt cacggaggct tctgtgtccg
     1921 attcttcagg tttcgtagat cacggtatcc cagtgaaagt cagcagtaca ttcagctctg
     1981 cagttggaat ggatgccaac ggaaaggctc atttctacca ggctcgtggg cagtccccta
     2041 cacctttctt tatactggac atctgccttg acacgcatga aacggttcaa gccaatacac
     2101 aggtgcctgg tgcctgtttc ccatggggca tgtgcgttgc cagcaatgga aaggtctatg
     2161 tcggaacagc cgatacaggg cacctctttg agtatgatcc gctgagcaaa cagttggttg
     2221 atcttggccg ccctggagag gagtcttaca tctggacgct ggcagaggga acagacaaga
     2281 agatttacgg tggaacctat ggcaggtgca aacttgtccg ctacgatcct gccacgggtc
     2341 agtccgatga tctggggaga atggatccaa cagagcagta cttgcgaagc gtcgtcgctg
     2401 attcggatgg ctatgtctat gctggcatcg gatccgcgcg agcaggactg atcgcctatc
     2461 acatcgagtc cgctacgtgg agccaactca ttcccgagga ccaacgagtc cctggttggg
     2521 gtgctgttca tatcggggag gatggacaat gttatgccac atctccggct ggcaatttcc
     2581 atctgacggg tggcatagct attccagtcg agactcctgc caaaaagtca ggatatctcc
     2641 ttccggacgg gagatctctg tccaaacacg atgctgaaaa gaactatgtt atcgtgaaat
     2701 ccgccaccga ttcacaggag atccgaattg actatgagat agagaatgcg ggaactcgaa
     2761 tctttgtcgt ggacaaagct ccggacggaa aggtatacgg aagcagtttt ctacccctgc
     2821 aactgtttgt ttatgatcct gagacagggc agtctcagaa tcttgggaaa ccgacggtct
     2881 ctgctgggga aatatactca ttcacgattt tcaagaatca acttg
//
LOCUS       DAOQUC010000369         2921 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_39947_length_2921_cov_2.632589, whole genome shotgun sequence.
ACCESSION   DAOQUC010000369 DAOQUC010000000
VERSION     DAOQUC010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2921)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2921)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2921
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39947_length_2921_cov_2.632589"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..140)
                     /locus_tag="PKH07_07440"
     CDS             complement(<1..140)
                     /locus_tag="PKH07_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNT34815.1"
                     /translation="MASEEIGKNQGFTLIELLVVVAIISLLTGIAVPNFLSAQTRSRA
                     ARA"
     gene            complement(145..720)
                     /locus_tag="PKH07_07445"
     CDS             complement(145..720)
                     /locus_tag="PKH07_07445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34816.1"
                     /translation="MASKRIQKQNPVASAVAVFFLLGVTLFGISRLPWFGYLRGETNT
                     KNPYDLSALERMSPERRDKQIQDWVDEWRNLPDDEKEIRADQYLDAVVDYTAKRLGFS
                     QSQKAETRGLIKSAALLAQDLRLEDRQLEDRREVIQDLRAQVLKEMDRVMSEEQRVKL
                     QELIEERRRMEPPRYAPLRDGAPIRRRTPQE"
     gene            946..1263
                     /locus_tag="PKH07_07450"
     CDS             946..1263
                     /locus_tag="PKH07_07450"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HNT34817.1"
                     /translation="MIELLKKRRDALERICTEYKVLRLEAFGSAVKDKAFDPKTRDLD
                     FLVEFLSLEIGEYAACYFGLLEALESLYQCHVDLVMLSAIKNKYFLQMIEPDREDIYA
                     ACL"
     gene            complement(1318..1824)
                     /locus_tag="PKH07_07455"
     CDS             complement(1318..1824)
                     /locus_tag="PKH07_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008083985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HNT34818.1"
                     /translation="MENILRGKAWKFGDNISTDHIAPGRYFHLRSQLDELAKHVLEDA
                     DAEFASKMAPGDFVVGGSNFGLGSSREHAPTIIKIAGVSAVIAESFARIFFRNCINVG
                     LPVVWLDTENIETGDELEVDLEKGILLNVTKGTKQEFPALPPVMTQILEDGGLAEHVR
                     KNGDLKLA"
     gene            complement(1875..>2921)
                     /locus_tag="PKH07_07460"
     CDS             complement(1875..>2921)
                     /locus_tag="PKH07_07460"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008084069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase/3-isopropylmalate dehydratase large
                     subunit family protein"
                     /protein_id="HNT34819.1"
                     /translation="PRKELSNDHMTLRAFAAKAGAVVCEIGRGVCHQVVAEQYAKPGE
                     ISIGADSHSCTAGALAGLGTGMGSTDVAIGIALGKTWLRVPETFRINVTGKLPKGVYA
                     KDIILHLIGKISADGATYMSLEFVGDTIEKMPMHERLVLSNMAVEAGAKCGLIASDDI
                     TKDYLAAQGRVADFKPLAGDPDAPCAREMTIQAETLVPTVAFPHTVDNTRTIDQAAGV
                     KIDQVVIGTCTNGRIEDLAIAAGILKGRHIAPHIRMLVIPASAQIYLQALEKGYLSAF
                     IEAGGTICNPGCGPCVGVHQGILGDGEKCLSTQNRNFQGRMGNPNGEIYLASPATAAA
                     TAIEGVIADPRKYL"
BASE COUNT          620 a          810 c          777 g          714 t
ORIGIN      
        1 gctcgtgccg ctcgggatcg agtttgcgca ctgaggaagt tcggcacggc gattccagtc
       61 agaagcgaga tgatcgccac gaccaccagc agctcaatga gcgtgaatcc ctggtttttg
      121 ccgatttctt ctgatgccat ggctctattc ctgcggtgtt cgccttcgga tgggagcacc
      181 atctcgcagt ggtgcatatc tcggaggttc cattcggcgc cgttcttcga tcaactcctg
      241 aagcttcacc cgctgctcct cgctcattac acgatccatc tccttgagaa cctgtgccct
      301 gagatcctgg ataacttctc ggcggtcttc caactgtcgg tcttccaggc gaagatcctg
      361 cgccagcaga gctgcactct tgatcaagcc tctggtctcc gccttctgtg actgagagaa
      421 accaagtcgt ttggcagtgt aatccacgac agcatccagg tactgatcgg cacggatttc
      481 cttctcatca tcgggcaggt tgcgccattc gtccacccag tcctgaatct gtttgtcgcg
      541 tcgctcagga ctcattcgct ccagtgccga caggtcatag ggattctttg tgtttgtttc
      601 tccccgcagg tatccaaacc aaggcaggcg cgagattccg aacagggtca ctcccaacag
      661 aaaaaagacg gcaaccgccg atgccactgg gttttgtttc tgaatcctct ttgaagccat
      721 cgttctctca ctggacgatc tatcgccgta agttgtaact gtttctgtca caggaatcat
      781 atagaaaacg gagacattgg caagtgcact ctgcgaggga tggggttgtt gtgtcagatg
      841 tctgatttaa gcaccgacaa gccggcttga cgtcgaggcg tcttttatag tctagtctgt
      901 catgttccgg ttttggtcag cagcaacttg gttgagaggc tggagatgat cgagttactc
      961 aagaaacggc gagatgcttt ggagagaatc tgcaccgagt acaaggttct tcggctggag
     1021 gcttttggct ctgcagttaa agacaaagcc tttgatccga agacaagaga tctcgatttc
     1081 ctcgtggagt tcttgtctct ggagattggt gaatatgctg catgctattt cggtcttctt
     1141 gaggcgttgg agtctctcta tcagtgccat gttgacttgg tcatgctgag cgcgatcaag
     1201 aataagtact tcttgcagat gatagagccc gatcgtgagg atatctatgc agcttgtctc
     1261 tgaggtgcag agcctttcgg atggataact gcgcaggctg cgccccatca ccacacgcta
     1321 tgccagtttc agatcgccgt ttttcctgac gtgttccgcc aaaccaccat cttcaagaat
     1381 ctgagtcatg acagggggga gggcagggaa ctcctgcttc gttcccttgg tcacgttaag
     1441 aaggataccc ttctccaagt ccacttccaa ctcgtcgccc gtttcgatgt tttccgtgtc
     1501 aagccacacg accggcagac caacgttgat acagttgcgg aagaaaatgc gggcaaagga
     1561 ctcggcaatg acagcactga caccggcgat cttgatgatc gtgggagcgt gctcacggct
     1621 tgaacccaac ccgaagttgc tgcccccaac gacgaaatcc cctggagcca tcttggacgc
     1681 aaactccgca tcggcatctt ccaacacatg ctttgccaat tcgtccaact gggagcgaag
     1741 gtggaagtat cttcctggcg cgatatgatc ggtcgaaatg ttatctccaa atttccaagc
     1801 tttgccgcga aggatgtttt ccattttgcc aaaacccttt cttcagtttg agtcggtgcc
     1861 aagtctcgac tgactcaaag gtattttctc ggatcggcga tgactccctc gattgccgtt
     1921 gctgcagcgg ttgctgggga cgccaggtag atctcaccgt tgggattgcc cattctgccc
     1981 tggaaattcc gattctgtgt ggacagacat ttctctccat ctccgagaat tccctgatgg
     2041 acgcccacac aaggaccaca ccccggattg cagatggtgc cacctgcctc gataaaagcg
     2101 ctcagatagc ccttttccag cgcctgaagg tagatctgcg ccgaagcggg gatgacaagc
     2161 atacggatgt gcggcgcgat gtgccgaccc ttcagaatgc cagccgcgat ggcaaggtct
     2221 tcaattcgtc cgttggtgca ggtgccgata accacctggt ctatcttcac gcctgctgcc
     2281 tgatcaatgg ttcgagtgtt atcaaccgta tgcgggaacg ccactgtggg gacgagagtc
     2341 tccgcttgaa tcgtcatctc tcgggcacac ggggcatccg gatcacccgc aagaggtttg
     2401 aaatcagcca ctcgcccctg agcggcaagg tagtccttgg tgatgtcgtc cgaagcgatg
     2461 agaccgcact tggcaccagc ctctaccgcc atattggaga gcacgagacg ctcgtgcatg
     2521 ggcatcttct caatggtgtc gccgacgaac tcaagagaca tataagtagc cccgtccgcg
     2581 gagatcttgc cgatgagatg aaggatgatg tccttggcgt acacaccctt ggggagtttc
     2641 ccagtgacgt tgatacgaaa tgtctccggc acacgcaacc aggtcttacc taaggcaatg
     2701 ccgatggcga catcggtgga gcccattccg gttccgagac ccgcgagagc gcctgccgtg
     2761 caactgtgag agtcagcgcc aatggaaatc tctccgggct tggcgtactg ctccgcgacg
     2821 acctgatggc agacgccgcg tccaatctca cacaccactg cgccggcctt cgctgcgaag
     2881 gcgcgaagcg tcatgtgatc gttggagagt tcctttctcg g
//
LOCUS       DAOQUC010000370         2916 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_40036_length_2916_cov_2.736106, whole genome shotgun sequence.
ACCESSION   DAOQUC010000370 DAOQUC010000000
VERSION     DAOQUC010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2916)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2916)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2916
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40036_length_2916_cov_2.736106"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..512
                     /locus_tag="PKH07_07465"
     CDS             <1..512
                     /locus_tag="PKH07_07465"
                     /inference="COORDINATES: protein motif:HMM:NF013989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RibD family protein"
                     /protein_id="HNT34820.1"
                     /translation="KDDPMLDCRLPAQERPWRQPLRVILDPDERTPEEAQVVQTTTQA
                     GTLLVLARPAQRAEVWRRLGVQTWVCPSERGILDLDALLGQLGQQKVTGLLVEGGTTT
                     HTYFWQKGAVDKYTVFVAPKIVGGDSALWPIRGEGCETIGQATVLDDVTVEAFGNDVA
                     ITGYCRRKE"
     gene            564..1214
                     /locus_tag="PKH07_07470"
     CDS             564..1214
                     /locus_tag="PKH07_07470"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008733456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HNT34821.1"
                     /translation="MFTGIVEEVGECLRLQASGGTAALEVLVGGIIADLKLGDSLSVQ
                     GVCLTVCRIAGNRVSLDVSGETLSRTTLGSLTPGQKVNLERSLAVNGRLGGHIVQGHV
                     DGIAVVQSLQMERDYATLRCRIPASFERYIAEKGSVALDGISLTVAEKSPAGVFAVAV
                     IPTTIQNTTLRFRKAGDRLNLECDVLAKYIESLLQGKQTRQGLSEGFLLEHGFLAG"
     gene            1246..2439
                     /locus_tag="PKH07_07475"
     CDS             1246..2439
                     /locus_tag="PKH07_07475"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HNT34822.1"
                     /translation="MERLTIEQAIQHISEGKMLIVTDDESRENEGDFIMAADRVTPEA
                     INFMATHGRGLICVAMKGERLKELDLPPMVQDNTAKMRTAFTVSVDASEGTTTGISAH
                     DRAKTIQVLMDPKSAPEDLSRPGHIFPLRAVDGGVLRRAGHTEAVVDLTRLAGLTPAG
                     VLCEIMDEDGSMARMPKLVELAKRFGVGIVTIQDLIAYRHRTEKLVHCELVTPLPTKF
                     GNFQLHLYSSELEADHHLALVKGTPKPEEPTLVRVHSQCLTGDILGSMRCDCGDQLGL
                     ALQMIEREGCGVLLYMRQEGRGIGLVNKLRAYVLQDQGMDTVEANLHLGFKADERDYG
                     TGAQILCDLGLKRIRLLTNNPKKRAGLEGYGLEIVERVPVRIGHNKMNERYLRTKKEK
                     LGHDL"
     gene            2472..>2916
                     /gene="ribE"
                     /locus_tag="PKH07_07480"
     CDS             2472..>2916
                     /gene="ribE"
                     /locus_tag="PKH07_07480"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009674421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HNT34823.1"
                     /translation="MAKTVEGNLVGEGKKFGIVLSRFNEFISGRLLEGCLDGLVRHGV
                     KDEDIVVHRVPGSFEIPLVTQKLAESRKYDAVICLGAIIRGATPHFDYVCNETAKGIA
                     STSLKYGVPVIFGVLTTDTIEQAVERAGTKSGNKGWDAAVAALEMS"
BASE COUNT          649 a          660 c          936 g          671 t
ORIGIN      
        1 gcaaagatga tccgatgtta gattgccggc ttccggctca ggagcgtcca tggcggcagc
       61 ctcttcgcgt gattctggat cctgacgaga ggacgccgga agaagcgcaa gtggtacaaa
      121 caacgactca agcgggtacg ttgcttgtat tggcgcgccc cgctcaaagg gctgaagtgt
      181 ggcgacgctt gggcgttcaa acttgggtat gcccatcgga gcgagggatt ctggatttgg
      241 atgctcttct tgggcaactc ggtcagcaaa aggtgacggg gctgcttgtg gaaggtggaa
      301 ccaccacaca cacatatttc tggcaaaagg gtgccgtgga caagtatact gtctttgtcg
      361 cgccgaagat cgttggtgga gattcggcgc tttggccgat tagaggcgaa ggctgcgaga
      421 ccataggtca ggcaacagtg ctcgacgacg tcacggtaga agcctttggc aatgacgtcg
      481 cgataacggg ctattgccgc aggaaagagt aggatcggtt ttgcatgcct tgcgtgttga
      541 tacttgcatg aaggagtaca agtttgttca ctgggatcgt tgaggaagtt ggcgaatgcc
      601 ttcgactgca ggcttcgggc ggcacagccg ctcttgaagt gctggttggg ggcatcattg
      661 ctgatctcaa actcggtgac agtttgagcg ttcagggtgt ttgccttacg gtttgcagga
      721 tcgccggcaa tcgcgtttct ctggatgtgt ccggagagac gctctctcga acgactctgg
      781 ggagtctgac ccctgggcag aaggtgaatc tggagcgttc cctggcggtc aacggtcgtc
      841 tgggcggaca tattgttcag ggacacgttg atggaatcgc tgttgtccag tcccttcaga
      901 tggagcgcga ctatgcgacg cttcgatgtc gaattccggc gtctttcgag cgctacatcg
      961 ccgaaaaagg ctctgtggct ctggacggaa tcagtctgac cgtggcggag aagagtcctg
     1021 ccggggtttt cgcggtagcg gtgattccca cgacgattca gaatacgaca ttgcgttttc
     1081 ggaaagccgg tgatcgattg aacttggagt gtgacgtctt ggcgaagtat attgagtctc
     1141 tgctgcaagg aaagcagact cggcaaggct tgtccgaggg ctttctcttg gagcacgggt
     1201 ttctggctgg ctgatttttc tacagtggtg tgaggagatg agtgcatgga gcgattgaca
     1261 attgaacaag cgatacagca tatctctgaa gggaagatgc tgatcgttac ggatgatgaa
     1321 tcccgcgaga atgaagggga tttcattatg gcggccgatc gagtgacgcc ggaagcgatc
     1381 aacttcatgg cgacacatgg ccgtggcctg atctgtgttg cgatgaaggg tgagaggctc
     1441 aaagagttgg atctgccacc catggttcaa gacaacacgg ccaagatgcg gacggctttt
     1501 acggtatccg tagatgcttc ggaagggacg acaacgggca tttccgctca tgacagagcc
     1561 aagaccattc aggtcttgat ggatccgaag tctgcgcccg aggacttgtc gcgccctggt
     1621 catattttcc cattgcgggc ggtggatggt ggcgttttga gacgtgccgg ccatacagag
     1681 gctgtcgttg atctcacgcg gttggccgga ctgactcccg ctggcgtgct ttgcgagatc
     1741 atggatgagg acggcagcat ggctcggatg cccaaactgg ttgaactggc caagaggttt
     1801 ggtgtgggaa tcgtgaccat tcaggacttg attgcctacc gacacagaac ggagaaattg
     1861 gttcactgtg aactcgtcac gccgcttcca accaagttcg ggaatttcca actgcacttg
     1921 tattcaagcg aattggaagc ggatcatcat ctcgcgcttg tcaagggtac accgaagcca
     1981 gaggaaccga ctctggttcg ggtgcactcg cagtgcctga caggcgacat tcttggctcg
     2041 atgcgctgcg attgtggcga tcagctggga ctggcattgc agatgattga gcgtgaaggc
     2101 tgtggggtgc tcttgtacat gaggcaggaa gggcgcggca tcggcctggt gaacaagttg
     2161 cgtgcctatg tgctgcaaga ccaaggcatg gacaccgtgg aggcgaatct gcatttgggt
     2221 ttcaaggctg atgaacgaga ctatggtacg ggcgcacaga tcctgtgcga tttggggttg
     2281 aaacgcattc gcttgttgac caacaatccc aagaagcgcg ctgggcttga gggatacggt
     2341 ctggagattg tggagcgtgt tccggtcaga atagggcaca acaagatgaa cgaaaggtac
     2401 cttcgaacca agaaggagaa actgggtcat gatctctgat cgcaggggat taaataatgc
     2461 aggaggcatc aatggcaaag acagttgaag gaaatctcgt cggtgaaggg aagaagttcg
     2521 gcatcgttct gtcgcgtttc aatgagttca tttcgggacg tcttctggaa ggatgtcttg
     2581 atggactggt gcgccacggg gtgaaagacg aagatatcgt agtgcatcgc gtcccagggt
     2641 cgtttgagat tccactggtg acgcagaaac tcgcagaaag caggaagtac gacgcggtca
     2701 tctgtctggg cgctatcatc cgaggggcaa cgcctcactt tgactacgtc tgcaatgaga
     2761 cagctaaggg aattgcctcg acctcattga agtatggcgt cccagtgatc tttggtgttc
     2821 tgaccaccga taccatcgaa caggcggttg agcgggctgg aaccaagagc ggcaacaagg
     2881 gctgggatgc agccgtcgcg gccttggaga tgtcaa
//
LOCUS       DAOQUC010000371         2913 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_40100_length_2913_cov_3.372288, whole genome shotgun sequence.
ACCESSION   DAOQUC010000371 DAOQUC010000000
VERSION     DAOQUC010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2913
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40100_length_2913_cov_3.372288"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            74..757
                     /locus_tag="PKH07_07485"
     CDS             74..757
                     /locus_tag="PKH07_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNT34824.1"
                     /translation="MRILVIEDEKKVANALDKGLRSEGYEVTSAATGEEGFFLLNAET
                     FDLVILDLMLPRRSGLEILSTIREKGVRTPVLILTARDGLEDKVEGLDRGADDYLVKP
                     FAFPELLARIRALLRRGVAEEPPILSAADLEMQLTHRKVIRSGQPIQLTAREFELLAY
                     LLQHKGHVVSREMIARDVWQEPNRAFSLDNVVDVHIARLRRKVDDPFPVKLLKTIRGV
                     GFIIEEGTQ"
     gene            754..2133
                     /locus_tag="PKH07_07490"
     CDS             754..2133
                     /locus_tag="PKH07_07490"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNT34825.1"
                     /translation="MMGTPLSIRRKLTLWYATALGLSLLVFSTALIGFVCSSMISQVD
                     TALKQDFLMIEKALEEDDDTERKEELDELSELNPVICFHLTAVSLTYASPRWHKESLD
                     DSLKAHRNHKRWYWESSRDNRYRLRTATLLVRGETHKVTAARSFDSVYRTIRALLIAM
                     AVTAPCTLILAMLGGSALAGRALAPIACMTNQARQITAENLSERLPVGKVDDELGRLA
                     QVLNDMLRRLQTSFESLRQFTADASHELRTPLTALRSIGQVGLQQPLDAAAYRDLIGS
                     MLEESDRLTHLVDSLLTLTRGESGHVQLTLEPTELGALVRDVAEWLSVLAEEKKQTFE
                     VSVSDSLPVSVDQPTIRQALLNLLDNAIRYTPDGGRIQVRAFADERGNAVVEVQDNGS
                     GIPKDQQSLIFERFYCLDKSRSREMGGTGLGLAIARWEVQANQGRIEVESTEGEGSIF
                     RVILPLRKE"
     gene            2241..>2913
                     /locus_tag="PKH07_07495"
     CDS             2241..>2913
                     /locus_tag="PKH07_07495"
                     /inference="COORDINATES: protein motif:HMM:NF022838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-like domain-containing protein"
                     /protein_id="HNT34826.1"
                     /translation="MMRKLGWIALSLLVAGFAIEALCEDKEEAISIEQLPAAVRTALE
                     AKTAGCTIVEIEKESEDGKVVYEAEYKTADGKKCEIELSEDGTILEEEDADEDDEDDG
                     DEEEVKVSLDQVPAAVKAAVEKAVANGTLIELTKETEDGKVVYEAEYKTADGKKREIE
                     MAEDGTVLEEEDADEDDEDDEDEEEEEVKVSLDQVPAAVKAAVEKAVANGTLIELTKE
                     TEDGKV"
BASE COUNT          743 a          714 c          861 g          595 t
ORIGIN      
        1 agattcttaa gggaatcttc agttgaccca gagcaatgac tgaagaagaa tttgtcacag
       61 aggagtacca atagtgcgga ttctggtcat cgaagatgag aagaaggtcg ccaatgcgct
      121 ggacaaagga ctgcgctcag agggctatga ggtaacatcg gcagcgacgg gcgaagaggg
      181 tttcttcctt ctcaacgccg agacctttga tctggtgata cttgatttga tgctccctcg
      241 gcgaagtggc cttgagattc tgagtacgat tcgagaaaag ggtgttcgaa cgcctgttct
      301 catcctgact gcgcgggatg gccttgaaga caaagttgag ggtcttgaca ggggagccga
      361 tgattatctg gtcaagccct ttgcgtttcc cgaattgctt gcgcgcattc gggctcttct
      421 ccgacgaggt gttgccgagg agcctcccat tctcagcgcg gcggatcttg agatgcaact
      481 gacccaccgg aaagtgatcc gatccggtca accgatccaa ctgaccgccc gcgagttcga
      541 actgcttgcg tatcttctcc aacacaaggg acatgttgtc tcgcgagaga tgatcgcccg
      601 cgatgtatgg caggagccca accgtgcatt ttctctggac aatgtcgtgg atgtccatat
      661 cgcacgactg cgaagaaaag tggacgatcc gttcccggtg aaactgctga aaaccattcg
      721 gggcgttgga ttcatcattg aggagggaac tcaatgatgg gaactccctt gagcattcgt
      781 cggaaactca ccctctggta tgcaacagca ctggggctga gtcttctcgt cttctcgaca
      841 gcactcattg gctttgtttg ttccagcatg atcagtcagg tagatacagc actcaaacag
      901 gatttcctga tgatcgagaa ggctttggaa gaagatgatg acacggaaag aaaagaagaa
      961 ctggacgaac tgtccgaact caatccggtg atctgctttc acctgacggc ggttagcctg
     1021 acctacgcca gtccgcgctg gcacaaggaa tctcttgatg attcactgaa agcgcaccga
     1081 aatcacaagc gctggtactg ggaatcctct cgggacaacc gataccgact tcgcacggcg
     1141 acgttgttgg ttcggggcga aacccacaaa gtcactgcgg cacggtcttt cgactccgtc
     1201 tatcgtacga tcagagccct tctcatcgcc atggcagtca cagccccctg cactttgatc
     1261 ctggcaatgt taggaggttc cgcactcgcg ggacgtgccc ttgccccgat cgcttgcatg
     1321 accaatcagg cacgacagat caccgcggag aatctctcag aacggttgcc tgtgggcaaa
     1381 gtggatgatg aactgggacg ccttgctcag gttctgaacg acatgctcag aaggcttcaa
     1441 acctcctttg aatcattgcg ccaattcacc gctgacgcat cccatgagtt gcgcactccg
     1501 ttgacggcgc ttcgaagtat cggacaggtc ggtcttcagc agcccttgga cgccgcggca
     1561 tacagagatc tgattgggag catgttggaa gaatccgatc gcctgacgca cctggtggac
     1621 agcctcctga cgctgactcg tggtgagtcg ggacacgtcc aactgacact cgagccaaca
     1681 gagcttggcg cactggttcg ggacgttgca gagtggcttt ccgttctggc tgaggagaag
     1741 aagcagacgt ttgaagtcag cgtctccgat tcacttcccg tttcggttga tcaacccacg
     1801 attcgacagg ctctgctgaa tcttttggac aacgcgattc gatacactcc cgacggtgga
     1861 cgaattcaag tgagagcatt cgcggacgag cgtggaaatg ccgttgtcga agtacaagac
     1921 aatggttctg gcatacccaa ggatcagcag tccctcatct tcgaacgttt ctattgtctg
     1981 gacaaaagcc gttcgaggga gatgggagga acaggtctgg gtctcgccat tgcgcgctgg
     2041 gaggttcaag cgaaccaagg acgcatcgaa gtcgaaagta ccgaaggcga aggcagcatt
     2101 tttagagtga tcctgccttt gagaaaggag taatgaaagg caggcacatc tcgggagttg
     2161 ccgtcccgcg tttgaagcaa gagccaaagg ctgccttacg gaaggcagca cacaataaag
     2221 gagcaaagga gcaaaagacg atgatgagga aactcggttg gatcgcattg agtttgttag
     2281 tcgcaggttt cgcaatagag gccttgtgtg aggacaaaga agaggcgatt tcgatagaac
     2341 agctgcctgc ggcggtgcga acggcactcg aagccaagac ggcaggatgt actatcgtgg
     2401 aaatcgaaaa ggagtcggaa gacggtaagg ttgtctacga ggcggagtac aagaccgctg
     2461 atggcaagaa atgcgaaatc gaactgtccg aagatggaac cattctcgaa gaagaggacg
     2521 ccgacgaaga tgatgaggac gatggcgacg aggaagaagt gaaggtctca ctcgaccagg
     2581 ttcccgctgc ggtgaaggcc gctgtggaga aggctgttgc caatggaacg ctgatcgaac
     2641 tgaccaagga aaccgaagac ggcaaggtcg tctacgaggc ggagtacaag accgccgacg
     2701 ggaagaaacg cgagatcgaa atggcggaag acggaaccgt cctcgaagaa gaggacgccg
     2761 acgaagatga tgaggatgat gaggacgagg aagaggaaga agtgaaggtc tcactcgacc
     2821 aggttcccgc tgcggtgaag gccgccgtag agaaggccgt tgccaatgga acgctgatcg
     2881 aactgaccaa ggaaaccgaa gacggcaagg tcg
//
LOCUS       DAOQUC010000372         2908 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_40204_length_2908_cov_3.171048, whole genome shotgun sequence.
ACCESSION   DAOQUC010000372 DAOQUC010000000
VERSION     DAOQUC010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2908
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40204_length_2908_cov_3.171048"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="PKH07_07500"
     CDS             complement(<1..272)
                     /locus_tag="PKH07_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HNT34827.1"
                     /translation="MKPYTDKAHIAVIGGSGLYSLEGMQMKESIQVDTPYGKPSGGIR
                     IGQLEGKTIAFLARHGEGHRFNPTHVPYRANIYALKSLGVRRILAVS"
     gene            complement(360..794)
                     /locus_tag="PKH07_07505"
     CDS             complement(360..794)
                     /locus_tag="PKH07_07505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34828.1"
                     /translation="MSLAAILDKGDIVPFGNIHHGVHVAGIAKGYEALRAVEESVVNG
                     TLQQLTLSHRERSVFVDLLGNLGSGEASCIAIAKERKGIVVADDSAARGVCHQWGCSV
                     TGTIGLLSDLRRERRLSPQQANGLLKEMIQHGFYSPVKKVPY"
     gene            735..1488
                     /locus_tag="PKH07_07510"
                     /pseudo
     CDS             735..1488
                     /locus_tag="PKH07_07510"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
     gene            1758..>2908
                     /locus_tag="PKH07_07515"
     CDS             1758..>2908
                     /locus_tag="PKH07_07515"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNT34829.1"
                     /translation="MNQEAALEIQTPGWMSELNPSQRLAVLHSGGPLLVLAGAGSGKT
                     RVITYRIAYLIEACGASPWNILAVTFTNKAAHEMKERVVSLLGERAEAVLVSTFHSLC
                     ARMLRRDAVSLGISPEFTIYDETDSLSVVQQAKAQVAGECALKPQRIRAKISAAKSEM
                     MTPEDLAQSLDKDYVEGQQLARIFAAYEEILRANQAMDFDDLLLVTIRLLTEVSDVRD
                     RYQRRFRHVLVDEYQDTNSAQYKILKLLTGGSSKPDLCVVGDDDQSIYRWRGARLNNI
                     LDFEKDFPGTRVVRLEENYRSTQTILNASNAMVAHNQKRKGKTLWTRNVQGAALQLLS
                     GDDEQQEAAQVVETILRERQSRGTGTGIAVFYRTNSQSRALEETLIQWRV"
BASE COUNT          682 a          762 c          826 g          638 t
ORIGIN      
        1 gaaaccgcaa gaatccttct cacaccaaga ctcttgagcg catagatgtt ggcacggtac
       61 ggaacgtggg ttgggttgaa gcgatgccct tctccgtggc gagccaggaa ggcgatggtc
      121 ttgccctcca actgcccgat gcgtatcccg ccggatggct ttccataggg ggtgtctacc
      181 tggatggact ctttcatctg catgccttcc aggctgtaca gtcccgagcc tccgatgacc
      241 gcgatatgtg ctttgtcagt atagggtttc acgacagtgc tacctccgaa taggaatcct
      301 gatccttggg aacaatagtg atactgcaag cgacaagtca aggcgatgaa agaatggggt
      361 cagtacggaa cctttttcac gggggaatag aagccatgct ggatcatctc tttgagcagt
      421 ccgtttgcct gttgtggcga gagacgcctt tctcgtcgca gatccgagag cagtcctatc
      481 gtccctgtga ccgaacaacc ccattgatgg cagacacctc gtgcagccga gtcatccgcg
      541 acaacaatac ctttgcgctc cttggcaatg gcgatgcaag aagcctctcc tgaaccgagg
      601 tttccgagca gatcaacgaa gacagaacgc tctcgatgcg acagagtcag ctgctgtagg
      661 gtgccattga caacggattc ttcgactgca cgcagtgcct catatccttt cgcaatgcct
      721 gcaacatgga cgccatggtg gatattgcca aaaggcacaa tatccccctt atcgaggatt
      781 gcagccaggc tcatgtaacg aggtacaagg ggcgctatcg ccgcgtttag cctgcagcag
      841 tcaaagcaca tgaccacggg cgacggtggc ctgacgatca ccgcccgtga ggactgggca
      901 gagcgtatgt ttctcttccg cgacaagggc tggacgcgca aacccggctg gggggcgcgt
      961 acttacgctt ttctggcacc caactaccgc atgacggaat tgcagggcgc ggtaggtctg
     1021 gctcagataa agaaagtgcg tggcgttgtc gagaaacgaa tggaattggg caaccgcctg
     1081 acggaactga tcgccgatgt tgctggtctg acccccgccc ccgttaccca aggatcggaa
     1141 cacagttact ggtcataccc gctgcatgtt gacggctggc cgaccgacgg atttgcaacc
     1201 gctctgaaga gggaagggat tgcggtaggc ctgggctaca tcggactgcc catcttcctt
     1261 tgcatggaag cgctggcttc caagaagacc tttgggaatt cgacgcatcc tttcgatggc
     1321 tgccacggtg gacggacgat tgaatacaca gaaggcatgt gcccccgcac agaacaactc
     1381 ttgcagcaga tgattaacat cggcttcaac gaaaacttca ctgtcaaaga cattgaagac
     1441 atctctggcg ctattcgcaa ggtggctgaa ctgctggaaa aggagtgagg ggtgtcttct
     1501 ggatagttgg gtggcctctt tgctcacatc tgcctgtggt tgctggaatc attgtcttgc
     1561 cgatagttgt gatgtccatt ccaaggaaac atcggaattc cgccaactcc tgccgaccac
     1621 cgaaaacccg cacagcaaag tgcggcttag agatttcgtc atccccagca actctgtcca
     1681 tgccacggga gagattctct cgttgcccct gggttggcaa cccactatac tgccctgcag
     1741 ttgattgagg acaaggaatg aaccaagaag ctgcgctgga aattcagacg cctgggtgga
     1801 tgtcagaact caacccgagc caacgacttg ccgtactgca ttcgggtgga ccgctgctcg
     1861 tgcttgcggg agccggcagt gggaagaccc gagtaatcac ttaccgtatc gcgtatctga
     1921 tcgaagcgtg tggagccagt ccgtggaata ttctcgccgt gacgttcacc aacaaagcgg
     1981 ctcacgagat gaaggagcgt gttgtttccc tgttgggaga gagggcggaa gcggtgctgg
     2041 tgagcacatt ccattccctg tgtgcgcgca tgcttcgaag agacgccgtt tcattgggta
     2101 tttcacccga gttcacgatc tacgatgaga ctgacagcct cagtgtcgtt cagcaggcca
     2161 aggcacaagt ggctggcgaa tgtgctttga aaccgcagcg catccgggca aagatatcgg
     2221 cggcgaaaag cgaaatgatg acgcccgaag atcttgccca gtctttggat aaggattatg
     2281 tggaagggca acagttggca agaattttcg cagcctacga agagattctc cgcgccaatc
     2341 aggccatgga tttcgacgat ctgttgttgg tgaccattcg acttctgaca gaagtttccg
     2401 atgttcgaga tcggtatcaa aggcgttttc ggcacgttct ggtggatgag tatcaagata
     2461 ccaacagtgc gcagtacaag attctgaaac tcctgaccgg tggcagttca aagccggact
     2521 tatgcgttgt aggagacgac gaccagagca tctatcggtg gcggggtgcc cgtctgaaca
     2581 acattctgga ttttgaaaag gatttccctg gcacccgggt ggtgcgtctt gaggagaact
     2641 atcggtctac ccaaactatt ttgaacgcct ccaatgccat ggtcgcacac aatcagaagc
     2701 gcaagggcaa aacgctctgg actcgaaatg tccagggcgc tgcacttcag ctcttgagtg
     2761 gagatgacga acaacaagag gcggcacagg tcgtggaaac cattctccgc gagcgacaga
     2821 gccgtgggac gggaaccggt attgccgtat tctaccgcac caattcacag tcccgtgcat
     2881 tggaggaaac gctgattcag tggcgtgt
//
LOCUS       DAOQUC010000373         2904 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_40275_length_2904_cov_2.965602, whole genome shotgun sequence.
ACCESSION   DAOQUC010000373 DAOQUC010000000
VERSION     DAOQUC010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2904
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40275_length_2904_cov_2.965602"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..392
                     /locus_tag="PKH07_07520"
     CDS             <1..392
                     /locus_tag="PKH07_07520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34830.1"
                     /translation="VRKRLLTTRQQAIAAVIKQAVNNLLSLPVEQYFQLLERKIKVLP
                     IQGTTELLLNPKDRQRVPPGFLERLNQSLANGSIVLSDETRDIDGGFILRTGRTEENC
                     SFEAVLQGIRDALEVEIGQALFGESKQ"
     gene            427..1431
                     /locus_tag="PKH07_07525"
     CDS             427..1431
                     /locus_tag="PKH07_07525"
                     /inference="COORDINATES: protein motif:HMM:NF014094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HNT34831.1"
                     /translation="MEWIEFQEDDRYVFAVARVRAMEARMLDDYFLSRLTSESPDGAM
                     RLLSDSVYRDVFAGIERLEDFAPALEDHLKLVFDELQGMAAEPALIQSLRARYDYHNL
                     GPVFMAFVRKTPLDAALLSKVGNVPVIDLASAVERQEDSLLREFFGAIWDKCLQVWNE
                     LPEIQAREKITVLLDDAYWESMTDEASHCENALLRRYYTLRLQWENLERIVRQMTLYQ
                     DGTSIRIDLFEESEWGNDVWRRLGVTPLDQLAQEVPMSLLKAPFAETLSLLIQEKRMD
                     GFLTGRHEWLKKLLETTSYVSFGPEPLIAFALKKEMEADRIRAALIRILYPVSKGQQG
                     "
     gene            1444..1755
                     /locus_tag="PKH07_07530"
     CDS             1444..1755
                     /locus_tag="PKH07_07530"
                     /inference="COORDINATES: protein motif:HMM:NF014092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HNT34832.1"
                     /translation="MARMAGVGSKVCLWPFRYFGIETFFVPEQGTAAEIMKNLIGSKD
                     YTVILVTEKVYREAQELIEAQRESYLPAITLIPDLEGSEGLALERITNAIRTAVGMDI
                     A"
     gene            1798..>2904
                     /locus_tag="PKH07_07535"
     CDS             1798..>2904
                     /locus_tag="PKH07_07535"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012996225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HNT34833.1"
                     /translation="MNEGRVVKVAGPLVVAENMHSARMYDVVHVSDQNLIGEIIELRE
                     DRASIQVYEETGGLGPGEPVRTTGEPLSVELGPGVLTSIFDGIQRPLDVIMQIAGTYI
                     TRGISVPSLDRKKKWAFKPIAQVGQQVTGGDFLGRVQETTLIEHRVMVPPNVAGKVIE
                     IHEGEFTVEETVAVLEAEDGQKISLTMMQKWPVRKARPYKRRIAPEIPLVTGQRILDV
                     FCPVAKGGTASVPGPFGAGKTVIQHQLAKWADAEVIIFIGCGERGNEMTDVLLEFPEL
                     KDPKSGEPLMLRTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYSVALMGDSTSRW
                     AEALREMSGRLEEMPGEEGYPAYLPSRLAEFYERA"
BASE COUNT          690 a          728 c          832 g          654 t
ORIGIN      
        1 aagtcagaaa acgccttctg accactcgac agcaggccat tgctgccgtc atcaaacagg
       61 cggtcaacaa tcttctttct cttccggtgg agcagtactt ccagcttctg gaacgaaaga
      121 tcaaagtctt gccgattcag ggtacaacgg aacttcttct gaatccaaag gacaggcagc
      181 gagtgccacc aggttttctg gagcggttga accaatccct tgccaacggg agtatcgtgc
      241 tttcagacga gacaagggat attgacggag ggttcatcct tcgaaccgga cgaacagagg
      301 agaactgctc attcgaagcc gttcttcaag gaattcgtga cgcactcgaa gtggagattg
      361 gacaggcgct gtttggcgag tcaaagcagt agttgttcgc gtttttcgga cagggtttag
      421 gtcgctatgg aatggatcga atttcaggaa gatgaccggt atgtgtttgc ggtcgcccgc
      481 gttcgcgcca tggaagctcg gatgttggat gactatttcc tctcgcggct cacttccgaa
      541 agtcccgatg gagcaatgcg tctcctgagt gattcggttt accgtgatgt gtttgccggc
      601 atcgagcgcc ttgaggattt cgcgcccgcc cttgaagatc atctcaaact ggtttttgat
      661 gaactccagg gtatggcagc tgaacccgcg ctcatccaat cattgcgcgc ccggtacgac
      721 tatcacaact tgggtcctgt tttcatggcc ttcgttcgaa aaactcccct cgatgcagca
      781 cttctgtcga aggtcggaaa tgttcccgtg atagacctcg cctctgcagt cgagcgtcaa
      841 gaagactccc tgctccgtga gtttttcgga gcaatatggg acaagtgcct gcaggtctgg
      901 aatgagctgc ccgaaataca ggccagagag aagatcaccg ttcttctcga tgatgcctat
      961 tgggaaagca tgacagacga ggcttctcat tgcgagaatg ctctgttgcg gcgctactac
     1021 acgctgaggc ttcagtggga gaatcttgaa cgaatcgtcc gacaaatgac tctttatcag
     1081 gacggcacaa gcatcaggat agatttgttc gaggaaagtg agtggggcaa cgatgtttgg
     1141 cggcgcttgg gggtcactcc actggatcag ctggctcagg aagtgccgat gagtcttctg
     1201 aaggctccat ttgccgagac gctgtctctg ctgatccagg agaaacgtat ggacggtttc
     1261 ttgacgggca ggcatgagtg gctcaagaag cttctggaga cgacctccta tgtcagtttt
     1321 ggtccagaac cgttgatcgc tttcgctttg aaaaaggaaa tggaagcaga ccgaatcagg
     1381 gctgccctga tcaggatcct gtatcctgta tccaagggac agcaaggtta agagaaggtt
     1441 tctatggcta gaatggctgg agttggttcc aaagtgtgtc tgtggccgtt tcggtatttc
     1501 ggcattgaga ctttctttgt ccccgaacag ggcaccgctg ccgagattat gaaaaacctg
     1561 atcggctcca aggactacac cgtcattctg gtgaccgaga aagtctatcg tgaggcacag
     1621 gagttgatag aggcacagcg ggagtcttat ctgcccgcaa tcacgttgat ccccgatctt
     1681 gagggcagcg aagggctggc tctggaacgg atcaccaatg cgattcggac cgctgtgggt
     1741 atggatattg cgtagaagaa acgctgcgat ctcaggctcg gaaaagggag cacgtctatg
     1801 aatgaaggca gggtagtgaa agtcgcggga cctctggtcg tcgcggagaa catgcacagc
     1861 gcaaggatgt atgacgtcgt tcacgtcagc gaccagaacc tcatcggcga gatcattgaa
     1921 ctgcgtgagg atcgcgcctc aatccaagtc tacgaagaga ctggtggact gggtcccgga
     1981 gagccggtca gaaccacagg agaacctctg agtgtggaat tgggccctgg tgtcctgacc
     2041 tccatcttcg acggaatcca aagacccttg gacgtgatca tgcagattgc gggaacctat
     2101 atcacgcgcg gaatctccgt cccgagtttg gatcgaaaga agaaatgggc attcaaaccc
     2161 atcgctcagg tcggccaaca ggtcactggt ggcgacttcc tgggaagagt ccaggaaacc
     2221 actctgatcg aacaccgcgt catggttcca ccgaacgtcg ctggcaaagt catagaaatc
     2281 catgaaggcg agttcactgt tgaggaaacc gttgccgtcc ttgaggctga agacggtcag
     2341 aagatttcct tgacaatgat gcagaaatgg cctgtccgaa aagccaggcc ctacaagaga
     2401 cgaatcgctc ctgaaattcc cctggtcacg ggtcagagaa ttctcgatgt cttctgcccg
     2461 gttgccaagg gaggcacggc gtcggtgccc ggcccctttg gcgcaggcaa aacggtcatt
     2521 caacatcagt tggcaaaatg ggctgacgcc gaggtgatta tcttcatcgg ctgcggagaa
     2581 cgtggcaacg agatgacaga cgtgcttctg gagttccccg agctgaaaga ccccaaatcc
     2641 ggggaacctc tgatgctcag gactgtgctc atcgccaata catcaaacat gcctgtggcc
     2701 gcacgcgagg cgtccattta cacaggcata accattgccg agtacttccg agacatgggc
     2761 tacagtgtcg cactgatggg ggattcaaca tcccgttggg cagaagctct tcgcgagatg
     2821 tcaggacgtc ttgaggagat gcccggtgaa gaaggctatc ctgcatatct gcccagccgt
     2881 ctggccgagt tctacgaacg cgcg
//
LOCUS       DAOQUC010000374         2898 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_40413_length_2898_cov_2.589870, whole genome shotgun sequence.
ACCESSION   DAOQUC010000374 DAOQUC010000000
VERSION     DAOQUC010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2898)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2898)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2898
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40413_length_2898_cov_2.589870"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..531)
                     /locus_tag="PKH07_07540"
     CDS             complement(<1..531)
                     /locus_tag="PKH07_07540"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNT34834.1"
                     /translation="MSQEVPRILVVDDELGIREGCRKIFSVEGYGVEVACNGLEGLET
                     FKSQGSFSVVLVDLNMPKMGGLELIEHLRELDDDVVIFVITAYATIETAVEATRKGAY
                     GYIPKPFTPDELLLPVRNGLERRALTLESKRLREERERRLLELAVERSRSSTIISGMS
                     DGVLVINRDRQIVLDAL"
     gene            complement(528..950)
                     /locus_tag="PKH07_07545"
     CDS             complement(528..950)
                     /locus_tag="PKH07_07545"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNT34835.1"
                     /translation="MAHPSTKILLVDDDPDFLEISRGILESAGYQVVVASSPEEAMPL
                     LHLEHPSLVITDLMMVSLDSGFRFVSEIRETEGFQTIPVVMLTAVASQRGFDLAPRSA
                     DDLRAMQVDAFIPKPVKPNSLLQTVASLLSKTGENSAR"
     gene            complement(1052..>2898)
                     /locus_tag="PKH07_07550"
     CDS             complement(1052..>2898)
                     /locus_tag="PKH07_07550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34836.1"
                     /translation="TIVNRQTKDLPAQVMLHFSPGAEFTTSPTLSVSGKRACVHIDDR
                     VLAIVDSDALPESEMVVSGNALSISGMMKAGSNAKVTACLPGWSLALERRAEVQLGDD
                     LFERFKEYWTRVMAPAMQVQLPDAWLEQVIRANQVHTLCAARNEDQGKRIVPWIASDR
                     YYTAIDSEGNSVVRGMMYWGHQDFAERSFEFFFSRYNPEGFMTTGYTLMGNGWHLWAL
                     GEFMRLADDRTWFLGVQSKPLELCRWIQAQIQKTRRLTSSGERVPEYGLMPPGVQADW
                     SNYAYYFYLNGYFYAGLKATGNVLKELGNPDAEAVLKTAEELRAEIVRAFRQVQAQAP
                     VVGLNNGTWVPYFPASVYTPGKMADFFPGQDGNRSWAYDVDLGAHHLVPQGVLSPTEP
                     AVDWMCDSLEDNDFLGEGWGGYPASLNHEDWFNMGGFAKVQPYYCRIAEIYALRDEVK
                     PFLRSYFNTLASLIDATALSIFEHFSNFCYNKTHETGYFLHQSRTLLLTEREEELWLA
                     PFVPQDWFDDGKTISVSNAPSRFGTVSYTIKSFLNAGYIEVVVEPFGERKPKQVVLRF
                     RHPQGLQIRQAILNGTKLLDVDTQSGTVRMTPISKVMSVRAEFASLEP"
BASE COUNT          702 a          813 c          739 g          644 t
ORIGIN      
        1 cagcgcgtcc agcacaatct gtcggtcgcg gttgataacg agcacgccgt cgctcatgcc
       61 gctgatgatc gtgctggatc gagagcgctc aacagcaagt tccagaaggc gtctctcccg
      121 ctcttcccga agcctcttgc tttccaaggt gagggcacgc cgctccagac cgtttcggac
      181 ggggagaagc agttcatcgg gcgtgaaagg tttgggaata tagccatagg ctcccttgcg
      241 tgttgcctcg accgcagtct ctatcgttgc gtaggcggtt atgacaaaga taaccacatc
      301 atcatcgagt tcccgaagat gctcgatcag ttccaaaccg cccatcttgg gcatgttcaa
      361 gtccacaaga acaacggaga aactcccctg gctcttgaaa gtctccagac cctcaagacc
      421 gttgcaggcc acctcaacac catagccttc aacggagaag atcttccgac atccttcacg
      481 aatgcccagc tcatcatcta caacgagaat tctgggaact tcctgactca tcgtgctgag
      541 ttttctcctg tcttgcttaa caaagatgcc acagtctgca gaagactgtt gggtttcaca
      601 ggtttcggga tgaaggcgtc cacttgcatg gcgcgcaggt catctgcact tcgaggtgcc
      661 aaatcgaagc cacgctggct tgccacagct gtcagcatga cgacaggaat ggtctgaaaa
      721 ccttcggtct ctcggatttc tgacacaaat cggaaccccg aatccaggga aaccatcatg
      781 agatccgtga tcaccaacga cggatgttca agatgaagca gtggcatggc ctcttccgga
      841 cttgatgcga cgaccacctg atagccggct gactcgagaa tgcctcgact gatctccaga
      901 aaatcgggat catcatctac gagcagaatt ttggtacttg gatgagccat ctgtcacatc
      961 tcacagcctt cagactctgg taaaatgagc atatccgatt cgcctgggca ttgcaagcaa
     1021 gcctactgac tgcacgactg ctttactgat ctcatggttc cagagaagcg aattcagctc
     1081 gaacgctcat caccttggag atcggagtca tccgaacagt tcccgactgg gtgtcaacgt
     1141 caagcaactt ggtaccattc aggatggcct ggcggatttg aagcccttgt ggatgcctga
     1201 atctgagaac aacctgcttg ggttttcgct caccgaaagg ctccacgacc acttcaatgt
     1261 atcctgcgtt gaggaaggac ttgatcgtgt aggagacagt accaaacctg ctgggtgcgt
     1321 tactgacaga tattgtcttg ccatcatcaa accagtcttg aggcacaaaa ggtgcgagcc
     1381 agagttcctc ttctcgctct gtgagcaaga gagttcgaga ctggtgcagg aagtacccgg
     1441 tttcgtgcgt cttgttgtag cagaagttgg agaagtgttc gaaaatggaa agcgctgttg
     1501 cgtcaatgag cgatgcgagc gtgttgaagt aacttctgag gaaaggcttc acttcatccc
     1561 gaagtgcgta gatctcagcg atgcggcaat agtagggctg aaccttggca aagcctccca
     1621 tgttgaacca atcctcatgg ttgagcgagg cgggatagcc tccccaaccc tcgccgagaa
     1681 agtcattgtc ttcaaggcta tcgcacatcc agtctacggc tggttccgtt ggactcagca
     1741 ctccctgagg aaccagatga tgagcaccaa ggtctacgtc ataggcccaa ctgcggttgc
     1801 cgtcctgacc tggaaagaag tccgccatct tacccggtgt gtagaccgag gccggaaagt
     1861 acggaaccca cgttccgttg ttgagaccaa caacaggtgc ctgcgcttga acctgtcgaa
     1921 acgcgcgaac aatctccgct cggagttcct cggcagtctt gagaacggct tctgcatcgg
     1981 gattgcccag ttccttcaag acattccccg ttgccttcaa acctgcgtag aagtacccgt
     2041 tgagatagaa gtaataggcg tagttgctcc agtccgcctg cactcccggc ggcatcagac
     2101 cgtattcggg cacccgttcg ccgctggaag tgagtctcct ggtcttctga atctgtgcct
     2161 gaatccaacg gcagagttcg agtggcttgg actgaactcc aagaaaccac gtgcgatcat
     2221 ccgccaagcg catgaactca cccaacgccc acagatgcca accgttaccc atcagcgtgt
     2281 agcccgtcgt catgaagcct tcaggattgt accgagagaa gaagaactca aaactccgtt
     2341 cggcaaaatc ctgatgtccc caatacatca tgccgcgaac gacagagttg ccttcactgt
     2401 caatggccgt atagtaacgg tctgaggcga tccagggaac aatacgcttc ccctgatctt
     2461 cgttacgcgc ggcacagaga gtgtgaacct ggttggctcg aatcacctgt tccagccagg
     2521 catcaggcaa ttgaacctgc atcgcaggag ccatgactcg agtccagtat tccttgaaac
     2581 gctcgaataa gtcgtcacca agttgaacct ccgcccgccg ttccaaagca agactccagc
     2641 ctggaaggca tgcagtcacc ttggcgttcg agccggcttt catcatcccg gaaatgctca
     2701 aggcgttgcc ggacaccacc atctccgatt caggaagagc atccgaatct acgatggcga
     2761 gaaccctgtc atcaatgtga acgcaagcac gtttccctga aactgagagg gtcggcgatg
     2821 tcgtgaactc agcacctgga ctgaagtgca acatgacctg agcaggcaga tcctttgtct
     2881 gacgattcac gatggtgt
//
LOCUS       DAOQUC010000375         2888 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_40623_length_2888_cov_3.484645, whole genome shotgun sequence.
ACCESSION   DAOQUC010000375 DAOQUC010000000
VERSION     DAOQUC010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2888
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40623_length_2888_cov_3.484645"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(202..1377)
                     /locus_tag="PKH07_07555"
     CDS             complement(202..1377)
                     /locus_tag="PKH07_07555"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HNT34837.1"
                     /translation="MQIRPAKKLDLFPVYLFDQLDQDKAKVEATGADVIDLSVGDPDR
                     ETPAHIVQALCEAARNPDHQHYPSYRGLPVFRKAIADWYEQVFRVTLDPDAEVLSLIG
                     SKGGIGHIPSALVDPGDVVLIPDPCYPAYKPGIVLADAKFESMPLYESNGYLPDLKAI
                     PASICKRAKLMILNYPQNPTAAVATREFFEEAVAFAEQNEIIIVHDAAYSQAYFDGER
                     VISFLEVPGAKDVGIEINSMSKTFNMSGWRVAYAVGNRRVLAALGKVKSNVDMGVFEP
                     IQIAAVAALRGSQDCVTQMRSLYQGRRDVLVEGLRSLGWKVEKPKATFFVWLPVPEKG
                     KPSGVFASEVLQKAHVLITPGKGFGDCGEGYVRIALTVEEERFREVIQRLKKSNFVY"
     gene            complement(1528..2763)
                     /locus_tag="PKH07_07560"
     CDS             complement(1528..2763)
                     /locus_tag="PKH07_07560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34838.1"
                     /translation="MGMVAAVAWRLFKRPMVVFVSGLIFLFLPIGFWWSRGYQSENWL
                     ILWLTWAFFLLLPAGGQDDSPGKETKRLVLAGLLSMLAVYTNMTALPYVLLFLVFVPL
                     RFGKRSLAFLSPFFLVGAAVFLVMLIYSEGTYIENVWSNQIGTYPKENTLQYFAEKLS
                     GQGVKILYLEGPMILLALLGLARYMRSTEVPARMFVTWYSLFCFGSILFVTKGGTMDY
                     IFSLAEPVLSVFAAWFLCDLIHELGLFRKGFWTQKANLVPALVLFPTILTVLTFPALR
                     FEAQTLAEETYENNTEGVLGVMNFIEQHSKPGELVLMPAYYAYLSGRRLPYDCSSSYM
                     WFMRWFNADRDGARDAKVERLLETVTGALRRKEIRIVFLHKRQFGVITPIRQAVEEHY
                     RPVSRLQTLNEDLEIFLPQ"
BASE COUNT          716 a          819 c          726 g          627 t
ORIGIN      
        1 tcatttgcgg agcaagcgtt caaggaagtg ggcatagccc acgcgcccgc aggtgctcac
       61 ctactctgaa tgcctgattt cggtcgaagc ctttcacctt tggcttttcc gccgtgtttc
      121 agaatcgtgt tgtggaagtc aaaatgtcgg gaacccctac gctgtttccc gcttcgtccg
      181 cgccaagtgt ttctttctcg atcagtatac gaagttcgat ttcttcagcc gctgaatcac
      241 ctcccgaaaa cgttcttcct caaccgtcaa ggcaatccga acatacccct caccacagtc
      301 gccaaaacct tttcctggag tgatgagtac gtgcgctttc tgcaagactt ccgaggcgaa
      361 cacccctgac ggtttgccct tctcgggaac gggaagccag acgaaaaacg tggcctttgg
      421 cttctcgacc ttccagccaa gacttcggag accctcaacg agcacgtccc gcctgccttg
      481 gtacagggat cgcatctgtg tcacgcagtc ctgagaacct cgcagcgccg cgactgccgc
      541 gatctgaatc ggttcgaaca ctcccatgtc cacattggac ttcactttgc ccaaggcggc
      601 gagaacccgt ctattgccca cggcataggc aactcgccag ccagacatat tgaaggtctt
      661 ggacatggag ttaatctcaa tgccaacgtc cttggctcca ggcacttcga ggaacgagat
      721 gacccgttcg ccgtcaaaat aggcctgact atatgcggca tcgtgaacga tgatgatctc
      781 gttttgctcc gcaaacgcga cggcttcttc aaagaattcc cgcgttgcca ccgccgccgt
      841 cggattctga ggatagttca gaatcatcag tttggctcgc ttgcatatcg aggccggaat
      901 cgccttcaga tcaggaagat acccattgga ctcatacaag ggcatggact cgaacttcgc
      961 gtcagccaac acgatgccag gcttgtaagc cggatagcac ggatctggga tcagcaccac
     1021 atctcccgga tcgaccagcg cggatgggat atgtccgatt ccacctttgg agccgatcag
     1081 agaaagcacc tcagcgtctg gatccaacgt gacacggaag acctgctcgt accagtctgc
     1141 aatcgcctta cggaatacgg gcaagccacg ataggaaggg tagtgctgat gatcgggatt
     1201 cctggctgct tcacaaagcg cttgcacaat gtgtgcggga gtctctcgat ccgggtcgcc
     1261 aacactcaaa tcaatgacgt cggctccggt ggcttccacc ttcgccttgt cctgatccag
     1321 ttgatcgaaa agatagacag ggaaaagatc aagtttcttt gccggacgta tctgcatcga
     1381 gtcattctcc tctgccagcc tgcgcctatc attgtatggg gcgcacttct tctaagaata
     1441 gatgggagaa accggattcc gcttgcgtcc agtttcctcc gagtcggcca gcacaccact
     1501 ctacatctca gatgatacac cattccacta ttgaggcaag aaaatctcca agtcttcatt
     1561 caaagtttgc aggcgcgata ccgggcggta atgttcctca accgcttggc gaatcggcgt
     1621 aatcacgccg aactgccttt tgtgcagaaa gacgattcgg atctcctttc gacgaagcgc
     1681 tcctgtgacg gtttccaaaa gcctttccac tttggcatcc ctcgcgccat cccgatcggc
     1741 gttaaaccac cgcatgaacc acatataact tgatgagcag tcatacggaa ggcgccgacc
     1801 actgagatag gcatagtacg cgggcatcag tacaagttcc ccgggcttgg agtgctgttc
     1861 aatgaagttc atcaccccaa gaactccttc tgtgttgttc tcataggttt cttccgcaag
     1921 tgtctgtgct tcaaaccgaa gtgccggaaa agtcagaact gtcagaatag ttggaaacag
     1981 aaccaatgcg ggaacaagat ttgccttctg tgtccagaag ccttttcgga acagtcccaa
     2041 ctcatggata aggtcacaga ggaaccaggc agcgaacaca gacagaacag gctctgccag
     2101 agagaaaatg tagtccatcg ttcccccttt ggtgacaaag aggatcgagc caaagcagaa
     2161 aagggaatac caggtgacaa acatcctggc tggaacctcc gtgctgcgca tgtatcgagc
     2221 caagcccagg agtgccagaa ggatcatcgg tccttcgagg tagagaatct tcacaccttg
     2281 accactgagc ttttcggcga agtattgcag ggtgttctct ttgggatagg tgccgatctg
     2341 gttgctccag acgttctcga tataggtgcc ttcggaataa atcagcatga ccagaaacac
     2401 tgctgcgccg accagaaaaa acggcgacag gaatgccagc gacctctttc caaagcgcag
     2461 aggcacgaag accagaaaca ggagaacgta aggcaacgcg gtcatgtttg tatagacggc
     2521 gagcattgag agcaaacctg ccagcaccaa tctctttgtc tctttgcctg gagagtcgtc
     2581 ctgtccccct gcgggcagga gcaggaagaa agcccacgtc agccacagaa tcagccagtt
     2641 ctctgactga tatcctcgac tccaccagaa accgatggga aggaaaagaa agatcaagcc
     2701 agacacaaag acaaccatcg gacgtttgaa caaacgccag gcaactgccg ccaccatgcc
     2761 catcgttgca agatgccaga ggagcagaaa aacttagtgg tggtgctcgc cattctggcc
     2821 ggtcccggcc gacaaggcca tgccgttgat ggtgttgatg gtgcccctga ccatgccgga
     2881 agcgctca
//
LOCUS       DAOQUC010000376         2887 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_40637_length_2887_cov_4.742232, whole genome shotgun sequence.
ACCESSION   DAOQUC010000376 DAOQUC010000000
VERSION     DAOQUC010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2887
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40637_length_2887_cov_4.742232"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            138..938
                     /locus_tag="PKH07_07565"
     CDS             138..938
                     /locus_tag="PKH07_07565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34839.1"
                     /translation="MKDKNKSKPKFTGNALDRDEFRKRFLMEMVCHPLSLYPFVLGAT
                     LLMASWALTLRSGIAVFAGLAAILGSVGAFCTRLLLGSELTARKVVEQLEKEVQDERE
                     RSLDDLERRLGSDEDPRTEAALRDLRTLAKAFRETREWSDSLKAQPTLDILSGVEQLF
                     ARCVVLLERTLTLWYTARKITSSEARKPILDQREQIIADIGESIKQLGSILAGIQAIR
                     VGDSGDRSDIRQIRHELDQSLEVAKMVEKRMQAFNQDIDSRIAETQEP"
     gene            998..1831
                     /locus_tag="PKH07_07570"
     CDS             998..1831
                     /locus_tag="PKH07_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020148611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34840.1"
                     /translation="MFKALFRWMKAVGYLMTGQIDAARRSLDINPHVVRAKYDEIVRE
                     KTGRIHQYKEAVAGLIAQQEGKMAKVRGLTEDVQRLERLKAGALAKAKQRVAQLPGLS
                     KEQIQEDEDYKTCLAAYNDFSSTLAEKQERIAELEADVQSYGKRITEHKVQLQQLVRE
                     IENLRAEATDAVADMITAQQEKEIADAFSGIAKDGTAEELQRMRQLRQEVKAEARISK
                     EIAGTETKVQEAEFLEYARSSSHQNEFDSLIGLATQTDEKKAADTEPVKESGTSLPLP
                     E"
     gene            1878..>2887
                     /locus_tag="PKH07_07575"
     CDS             1878..>2887
                     /locus_tag="PKH07_07575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34841.1"
                     /translation="MLRFRFRTGVLLLALFGLLVVWDRQSPAETPSFSLAWSEYPSWS
                     AFGVAHEVKLINGKKGEMGPIETKWGVDIELKEADYDPCLVMYGANQCDAVCITNMDV
                     LNPSLSRKSVAILPTSTSAGADACIVTNDIASVQDLKGKNVYGLAKTVSEYCFVRNLE
                     ILGEKEKDYTFVNMDPAAAALAMQQREPGYQAIVVWNPFVLSTLSKRNDVKVLFDSTA
                     IPGEIIDMVVVAQATLDKPGGAKFACAIIDAFYALNKRIADPATHDDTLVALGEKFSN
                     LDLESMKTVVKQTKFYATPEEGIQVLNDLGTKDTMKKVVDFCVSHEIVAQAPVLGCGA
                     KSA"
BASE COUNT          731 a          689 c          855 g          612 t
ORIGIN      
        1 gtcggccagc gccgactggc aggtgggcta tgcccacttg tacgaataga ctgctcgtag
       61 caggaatcag gtctttttga agttgggtcg ccaagagacg gccagctgag cgacaagaag
      121 acaacaaaga tacgtccatg aaggacaaga acaagtcaaa accaaagttc acgggaaacg
      181 ctcttgatcg agatgagttt cgcaagagat tcctgatgga gatggtgtgc catccgcttt
      241 cgctctatcc ctttgtgctt ggagcgacac tgctgatggc aagttgggcg cttacgcttc
      301 gatcgggtat cgcggttttt gcgggacttg ctgcgatact gggttcggtc ggggcatttt
      361 gtacgcggct cctgctgggc agtgagttga cggccaggaa agtcgtggag caactggaga
      421 aggaagtcca ggatgagcga gaaaggagtc tcgacgatct cgagcggcgc ttgggcagcg
      481 acgaagaccc tcgcaccgaa gcggctctgc gcgatcttcg aacgctcgcg aaagcatttc
      541 gcgaaacccg agagtggtcg gactccctga aagctcaacc gacgctggat atcctttcgg
      601 gcgttgagca gctgttcgct cggtgtgtcg ttttgcttga gcgaacattg acgctctggt
      661 acactgcccg aaagataacc tcttcggaag caaggaagcc gattctcgat caacgggagc
      721 agattatcgc cgacatcgga gagagtatca agcagttggg cagcatcctt gctggcattc
      781 aggcgatccg agttggagac agtggcgatc gatctgatat caggcaaatt cggcatgaac
      841 tggatcaaag cctggaagtg gcgaaaatgg ttgagaagcg catgcaggca ttcaaccagg
      901 acatagattc gagaatagcg gaaactcaag agccttgacc ctgttgcggc ttggttctgc
      961 aggtttgttc tgagttctgc gaaaggagac agagaatatg ttcaaagctt tgtttcgatg
     1021 gatgaaggca gtggggtacc tcatgactgg tcagattgac gccgcccgac gttccctgga
     1081 tatcaatccc cacgtggttc gggctaagta tgatgagatc gttcgggaga aaaccggccg
     1141 aattcatcag tacaaggaag cggtggccgg actgatcgcc cagcaggaag gcaaaatggc
     1201 caaagtgcga gggctgaccg aggacgtgca gaggctggaa cgcctgaagg caggcgcact
     1261 cgcaaaagcc aaacagcgcg tcgcacagct tccggggctc tctaaggagc agattcaaga
     1321 ggatgaggac tacaaaacgt gccttgctgc gtacaatgat ttctcttcga cgctggcaga
     1381 gaaacaggag cgcatcgcgg agctggaggc tgatgtacag tcctatggca agcgaattac
     1441 cgagcataag gtgcaactgc agcagttggt acgtgagatc gagaacctcc gtgctgaagc
     1501 cacggatgcc gttgccgaca tgatcaccgc acagcaggag aaggaaattg ccgatgcctt
     1561 ttcgggtatt gccaaagacg gcacagcaga agaactgcag cggatgcgcc agttgcgtca
     1621 ggaagtgaaa gccgaagccc gcatctccaa ggagatcgcc ggcacggaaa ccaaagttca
     1681 ggaagctgaa tttttggaat atgcgcgcag ctcatcccat caaaatgagt tcgattccct
     1741 gatcggtctg gcaactcaga ccgatgagaa aaaggcagcg gacaccgaac cggtgaaaga
     1801 atcagggaca agcctgcctt tgcctgaatg aacagaggac acaaagagtc caatagagaa
     1861 aaaaaggaga aagagtcatg ttgagattca gattccgtac tggtgttctg ctacttgctt
     1921 tgttcggatt gctggtggtt tgggacaggc agtctccagc tgaaacgcct tcgttctcac
     1981 tggcatggag tgagtatcct tcctggagcg cgtttggtgt tgcccatgag gtcaaactga
     2041 tcaacggcaa gaagggcgag atgggtccca tcgagaccaa gtggggcgtg gacattgaac
     2101 tcaaagaagc cgactacgat ccgtgcctgg ttatgtatgg cgcgaatcag tgcgatgccg
     2161 tctgcattac gaacatggat gttctcaacc cgagtctgag ccgaaagtcg gtcgcgattc
     2221 tgccgacctc tacgagtgct ggggcggatg cctgcattgt gaccaatgac attgcctcag
     2281 ttcaggatct gaagggcaag aatgtctatg gccttgccaa gacagtttcc gaatactgct
     2341 tcgttcgaaa tctggaaatc ctgggtgaga aagagaagga ctacaccttt gtaaacatgg
     2401 atcctgccgc cgcggcgctt gcgatgcagc agcgtgagcc tggatatcag gcaattgtgg
     2461 tttggaatcc gttcgttctc tcgaccttgt ccaaacgaaa tgatgtcaag gttctctttg
     2521 attcgaccgc catcccggga gagatcattg acatggtggt cgtagcccag gcgacactgg
     2581 acaaacccgg gggagctaag tttgcgtgtg ccatcattga tgcgttctat gcgctcaaca
     2641 aacgcattgc cgatccggca acacacgatg acacactcgt ggcgcttgga gagaagttct
     2701 cgaatctcga tctcgaatcc atgaagacgg tcgtcaagca gacaaagttc tatgccactc
     2761 cagaagaagg catccaggtt ctcaacgatc tgggcaccaa ggataccatg aaaaaagtag
     2821 tggatttctg tgtcagccat gagatcgttg ctcaggctcc ggttctcgga tgtggagcca
     2881 agtcggc
//
LOCUS       DAOQUC010000377         2887 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_40639_length_2887_cov_3.570975, whole genome shotgun sequence.
ACCESSION   DAOQUC010000377 DAOQUC010000000
VERSION     DAOQUC010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2887
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40639_length_2887_cov_3.570975"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1316
                     /locus_tag="PKH07_07580"
     CDS             <1..1316
                     /locus_tag="PKH07_07580"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNT34842.1"
                     /translation="GGRIVDDSSKEHPMNVWTLAIREILHRKGLFALGVIAVAVAVGV
                     LVGSVELLRAHDLQTASILTQRQDEMEETFIVMQDDYRKIMKKLGFNLLILPQEQSLE
                     EFYANGALSHFMPQEYSDRLARSGLATIQHLLPLLEQQIQLPEFGDRAVLLVGVKGEV
                     PQLGQPSKEPIMVSVPDGAAVVGYAIWKNLGLKPGDGIKVLESEFRVAECLEERGSKD
                     DISIWINLNKAQQLLDKPDLINAIQALKCHCQGSDLPNIRKQIAGVLPGTQVIEFASE
                     VITRAEARDTAKAVAEAMIEQERINRAQLRETRESFVSWLIPVVIIGSGFLVAVLAFG
                     NVRERRAEIGILRAIGVASKQVLQLFLWRGVLIGLLGAPLGFLAGCISAAFLGAEAPG
                     SIQFICDPRLLLMVWAAAPILAAFASWPPAMAASQQDPALILREQ"
     gene            complement(1373..2104)
                     /locus_tag="PKH07_07585"
     CDS             complement(1373..2104)
                     /locus_tag="PKH07_07585"
                     /inference="COORDINATES: protein motif:HMM:NF039185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HNT34843.1"
                     /translation="MWGLVEHFMNALFPPLCRVCRCGLTPPFDASRVCDACLRKIRLL
                     QPPYCPTCGRPVATDALSPRLKTCEGCPPTKIYFDMARAWLEYGGVTADLIKLLKFRY
                     KKDILPMLVDFLLRAYEDQYKEISFDLIMPVPLHWMRLRYREFNQSELLATALGGTVG
                     VDVELEALCRIRRTPPQAQQSRDKRYSNVRGAFDVVVPEAVHGKSILMVDDVYTTGTT
                     VNECARVLKLAGASRVYILTIARAL"
     gene            complement(2122..>2887)
                     /gene="pelF"
                     /locus_tag="PKH07_07590"
     CDS             complement(2122..>2887)
                     /gene="pelF"
                     /locus_tag="PKH07_07590"
                     /inference="COORDINATES: protein motif:HMM:NF038011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GT4 family glycosyltransferase PelF"
                     /protein_id="HNT34844.1"
                     /translation="KLLTWVFKMIERINARITHRIVACSEKVRQDLIQFRVAPQEKIV
                     TIPYGFNLSRFARAAACPGKIRAELGVSTSVKVVGIVARLVPIKGHEVFLQAARNLVE
                     KMPESELEFWIVGDGELRESIKSQVTHLGLTSRVRFLEWRQDIESVYADLDVLALTSF
                     NEGLPLVIIEAMAASRPVVATNVGGVPEMISEGETGFVVPVGDSEAIAEGLSRILGDP
                     NRAKAMGVLGQKLSFEKYGIERLVRDLEALYESLLK"
BASE COUNT          665 a          794 c          775 g          653 t
ORIGIN      
        1 gtggcgggcg catcgttgat gattcgtcga aggagcatcc aatgaacgtc tggactcttg
       61 cgatccgaga gatccttcat cggaagggac tttttgccct gggcgtcatc gctgtcgctg
      121 tggcggtagg ggtgcttgtc ggatcggtcg aactccttcg ggcgcacgat cttcaaacag
      181 ccagcattct cactcagcgt caggatgaga tggaagaaac tttcattgtc atgcaggacg
      241 attatcgaaa gatcatgaag aagttgggct tcaatcttct gatcctgccc caggagcaat
      301 ccctggagga gttctatgcc aacggagcct tgtcacattt catgccgcag gagtattcag
      361 atcggttggc acgctccggt ctggcaacca ttcaacacct tcttcctttg cttgagcaac
      421 aaattcagct gccagagttc ggagaccggg cggtgcttct cgtgggcgtt aagggtgaag
      481 tccctcagct gggtcagcca tccaaggagc cgatcatggt ctccgttccg gatggcgcag
      541 cggttgttgg atatgcgatc tggaagaatc tgggtctcaa gcccggagat gggatcaaag
      601 tgctggagtc cgaattccgt gttgccgagt gtctggaaga gcgcggaagc aaggatgaca
      661 tcagcatctg gatcaatctc aacaaggcac agcaactcct cgacaaaccc gatctcatca
      721 atgccattca ggccttgaag tgccattgcc agggctctga tcttcccaac attcgaaaac
      781 agattgcggg agttcttcct ggcacacagg tcatcgagtt tgccagtgaa gtcatcacac
      841 gcgcggaagc acgagatacc gcaaaagccg ttgctgaggc aatgatcgag caggaacgaa
      901 tcaaccgagc acagctgcgg gagacgaggg agtctttcgt ttcctggttg attcctgtgg
      961 tgattattgg gagtggtttc ctggtcgcag ttcttgcctt tgggaatgtc agagaacgca
     1021 gagcggagat cgggattctc cgtgcgatag gagttgcctc aaagcaggtg cttcagttgt
     1081 tcctatggcg cggggtgctg atcggccttt tgggtgcacc cttgggcttc ttggcaggct
     1141 gcatcagcgc tgcctttctc ggagcagaag cacccggctc gatccagttc atctgtgatc
     1201 cgaggctctt actgatggtc tgggctgctg ctccgattct ggcggctttt gccagttggc
     1261 caccagcgat ggccgcttcg caacaagatc ctgctctgat tctcagggaa cagtaaaccg
     1321 ccagacttgg ggacggattc gacggaagaa ctcattcctt ggtacacaaa cgtcagagcg
     1381 ctcgcgcaat ggtgagaatg taaactcgag acgcaccggc aagtttcaga actcgggcgc
     1441 attcgttgac cgttgttccc gttgtgtaga catcgtccac cataaggata ctctttccat
     1501 gaacggcttc gggcacgaca acgtcaaacg ccccacgaac gttggaatag cgtttgtctc
     1561 gactttgctg tgcttggggt ggtgttcttc tgattcggca gagtgcctcc agttccacat
     1621 caacgccaac tgtgcctccc agtgccgttg ccaatagttc tgactggttg aactcccgat
     1681 accgcaaccg catccaatga agcgggaccg gcattatgag gtcgaatgaa atctctttgt
     1741 attgatcctc gtaggctcga agcagaaagt ccacaagcat cggaaggatg tccttcttgt
     1801 agcgaaattt gagaagcttg atcagatcgg cagtcacccc accatattcc aaccacgccc
     1861 gcgccatgtc gaagtaaatc ttggttgggg gacaaccctc acaggtcttg agccttggag
     1921 agagagcatc cgtcgcaaca ggtcttccac aggtggggca gtagggtggc tgaagaagtc
     1981 gaatcttccg cagacacgca tcgcagacgc gtgaggcgtc gaacggcggc gtcagcccac
     2041 agcgacaaac ccgacacagg ggcgggaaca aggcgttcat gaaatgctca acgagacccc
     2101 acataatctg caaccatcca cttatttcag aagagactca tagagtgctt ccagatcgcg
     2161 cacgagacgc tcaatgccgt atttttcaaa ggacaacttc tgaccaagca cccccatcgc
     2221 tttggcgcga ttcggatctc caagaatccg ggagagcccc tccgcgattg cctctgaatc
     2281 cccgacggga acgacaaagc ctgtctcgcc ctcagatatc atttcgggaa cgccacccac
     2341 gttggttgcg acaaccggtc gcgatgccgc catggcttcg atgatcacca gtggaagccc
     2401 ctcattgaaa gacgtcagtg cgagcacatc cagatccgcg tagacggatt cgatatcctg
     2461 tcgccattcg agaaaccgaa ctctcgatgt caaacccaga tgagtcacct ggctcttgat
     2521 ggattcccgc agttccccat cacccacgat ccagaactcc agttccgatt ccggcatctt
     2581 ctccacaagg tttcgtgctg cttggagaaa aacttcatgc cctttgatgg gaacaagccg
     2641 ggcgacaatg ccaaccacct tgacactggt ggaaacacct aactccgccc gaatctttcc
     2701 aggacacgct gctgcccggg caaatcggct cagattgaaa ccgtagggaa tggtgacaat
     2761 cttttcctgc ggcgcgacac ggaattggat caaatcctga cgaacctttt cactgcacgc
     2821 cacaattcga tgcgtgattc gagcattgat ccgctcgatc atcttgaaaa cccaggtgag
     2881 cagcttc
//
LOCUS       DAOQUC010000378         2885 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_40681_length_2885_cov_2.587279, whole genome shotgun sequence.
ACCESSION   DAOQUC010000378 DAOQUC010000000
VERSION     DAOQUC010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2885)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2885)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2885
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40681_length_2885_cov_2.587279"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            262..1575
                     /locus_tag="PKH07_07595"
     CDS             262..1575
                     /locus_tag="PKH07_07595"
                     /inference="COORDINATES: protein motif:HMM:NF025065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HNT34845.1"
                     /translation="MGNTELFGKIFDCECGKKHHIQPATVVLSENAVEAMPLEIRRLD
                     IGTSAVVLMDHRTQEVAGEAILDALNRSGFRAMRNVVPDDSRGNSPVCDDVTKSALVS
                     EIPQADFFVSVGSGVITDLAKWISFDRNIPFVVFATAASMNGFTSANVAPTIKGLKTL
                     VRAEPPRAVFSSPKVLCEAPYEMTASGLGDILAKSVSSADWRLNNLLFGDYYCARSVN
                     LIADIEPLYLNHPQDILNRRSSAIEALFQGLLLTGAAMTMAETSSPSSGGEHLISHTL
                     DMLTHIDGTPHDLHGRQVGVGTILAAELYDRILRCESPTFGIPSDGIDRDFWGVVADV
                     VENDYRLKAPRLESAVSTLSTGNTWDALRKELASLLRPPSVIHSCLSGASAAYLPEHI
                     GCSRERLLNVFLHAHEVRSRFTVLDLARLVGVLPSAAEEIIDQWM"
     gene            1591..>2885
                     /locus_tag="PKH07_07600"
     CDS             1591..>2885
                     /locus_tag="PKH07_07600"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNT34846.1"
                     /translation="MSKPNVIVCLCDQLRAFEVGCYGNSVVRTPNIDRLAENGVRFET
                     ACSNNPVCSPSRASLLTGQYSRTCAGMLLNADFHPPLKQRTQLLNTTMPEVFRNAGYH
                     TSLIGKWHIKPHPLTVGFDDALFPCFRHRYTGQTFIRNFGEDFPVEDYSPEYEIEQVS
                     RLLQENTERPFFLYYNISPPHMPLHDVPEFYRTLYGRDDVPLRKNVWRDGRMPWDERW
                     FKIYLWDFLYYLHHQPHTERLPDGFDLRDLTALYYGLTSWVDDLVGRLIDELKARGLL
                     DNTLIVFTSDHGDNLGSHHLFNKGSLIEESIRIPLIFHFPQRLHTLLNRSQVASIVDV
                     MPSVLGLLGIPIPEGVQGTDLSTVLLGQHNSVGENVAYIETYPGEIGIRTATHLYGIK
                     ADVKNRQGNECTVLDDRYMFFDLDNDPYELNNIAMTEDNA"
BASE COUNT          686 a          772 c          702 g          725 t
ORIGIN      
        1 gtcttatgag aaaattcaac aagagatttc aggatgaggg atttcccttc atcaggaaga
       61 atctgcaaga cgcaaaagag ccgagagaag acttccgagc agacgactgg cgttcccagg
      121 gtgctaattg tctctcattc gctgtatcgt gcccttgaac aaatcggagc gacaaatcat
      181 gtgctttctg gtagattcaa gaaactaatt cattctcgcc cagccaaggg tgagtacatc
      241 tcattgaaat ggaaaggtca gatgggaaat acggaactat ttgggaaaat ctttgattgt
      301 gaatgtggga agaagcacca tatccagcct gcaacagttg ttctctcaga gaatgccgtc
      361 gaggcaatgc ctctcgagat caggagactc gatatcggga caagtgctgt tgtgctgatg
      421 gaccacagaa ctcaggaagt tgccggtgag gcaatactgg acgctctcaa ccgttcagga
      481 ttccgtgcga tgcggaatgt tgttcccgat gacagcagag gtaactcacc ggtgtgtgac
      541 gacgtcacaa aatctgctct cgtatctgag attccccaag cagatttctt cgtctccgtc
      601 ggcagcggcg ttatcacaga tctcgcaaaa tggatctcct ttgatcgaaa tatcccgttt
      661 gtcgtttttg ccactgcggc aagcatgaat ggcttcacgt cggcaaatgt tgcaccaacc
      721 atcaagggct tgaaaacatt ggttcgcgct gagccaccac gagcagtctt ctcctctcca
      781 aaggttctct gtgaggctcc ctatgagatg accgcatcgg gtctggggga cattctggca
      841 aaatccgtca gttccgctga ctggcgattg aacaatctcc tttttggaga ttactactgc
      901 gcccgatcgg tgaatctgat cgcggatatc gagcccctct atctcaacca tcctcaggat
      961 atactcaaca gaagatcttc tgccattgaa gccttgtttc aagggcttct tttgacgggt
     1021 gcagccatga ccatggctga gacttcgtca ccttcgtctg ggggagaaca cctgatctcc
     1081 cacacactgg acatgctgac ccatattgat ggcactcctc acgatctgca cggaaggcag
     1141 gtcggtgtgg gaactattct tgccgctgaa ctctacgatc gcatactgcg ttgtgagtcc
     1201 cccacttttg ggattccatc cgatggaatt gacagagatt tctggggagt tgttgctgat
     1261 gtggttgaga atgactacag actcaaggca ccgagacttg aaagcgctgt gtctacactc
     1321 tccactggga atacatggga tgcacttcgc aaggagcttg cctcgttgct ccgccccccc
     1381 tcggtgattc acagttgcct ctccggagcc agcgcagcat acctgcccga gcatatcggt
     1441 tgcagtcggg agagactgct caatgtcttt ctccatgccc atgaagtccg atctcgattc
     1501 actgttctcg atctcgcccg cctggttggt gtgctcccga gcgctgccga ggaaatcatc
     1561 gatcagtgga tgtaggagtc tcccagtcca atgagcaaac caaacgtcat cgtctgtctg
     1621 tgcgatcaac tgcgggcatt tgaagtgggt tgctacggca actcggtcgt ccgcactccg
     1681 aatatagacc gactcgccga gaacggagtt cgttttgaga ccgcctgttc caacaatccg
     1741 gtatgcagcc cttcgcgtgc atcgttgctg accggtcagt acagccgtac ctgtgcaggg
     1801 atgcttctga atgcggattt ccatccccct ctcaagcaac gaacacagct tctcaacacg
     1861 acgatgcccg aagtatttcg gaatgcaggt taccacacat cgctgattgg aaaatggcat
     1921 atcaaaccgc atccgctcac agtgggcttt gatgacgctc tctttccctg cttccgtcac
     1981 cgttacacag gacaaacgtt cattcgaaac ttcggggagg actttcccgt tgaagactat
     2041 tcacccgagt acgaaatcga acaggtgagt cgtcttcttc aagaaaacac cgagcgtccc
     2101 ttcttcctct attacaatat ctcaccgcct catatgccgc tgcacgacgt acctgagttc
     2161 tatagaaccc tgtacggtcg cgatgatgtt cctctcagga agaacgtctg gagagatgga
     2221 aggatgccct gggatgaacg ctggttcaag atttacctct gggactttct ctactatctt
     2281 catcatcagc cgcacacgga aaggttgcca gacggttttg accttcgtga cctgacagcg
     2341 ctttattacg gtctgacaag ctgggtagat gatctggtcg gtcgcctgat tgacgaactg
     2401 aaggccagag gccttctgga caacactctg attgtcttca cttctgacca tggggacaac
     2461 ctgggaagcc atcacctctt caacaaaggt tccctgatcg aggagtctat tcggattcca
     2521 ttgatcttcc attttcctca gcgtctgcac actcttttga atcgttcaca ggttgcctca
     2581 atcgttgatg tcatgccctc agtgcttgga ctcctgggca tcccgattcc ggaaggcgtt
     2641 cagggaacag acctctcaac cgtcctcctc ggccaacata actcggtagg cgagaacgtc
     2701 gcttatattg aaacctatcc cggtgaaatc ggcatccgaa cagcaaccca tctctatggc
     2761 atcaaggctg acgtcaagaa tcgccaagga aatgaatgta ctgtccttga tgaccgatat
     2821 atgttctttg atctggacaa cgatccctat gagctcaata acattgccat gacagaagac
     2881 aatgc
//
LOCUS       DAOQUC010000379         2883 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_40708_length_2883_cov_3.476308, whole genome shotgun sequence.
ACCESSION   DAOQUC010000379 DAOQUC010000000
VERSION     DAOQUC010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2883
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40708_length_2883_cov_3.476308"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1028)
                     /locus_tag="PKH07_07605"
     CDS             complement(<1..1028)
                     /locus_tag="PKH07_07605"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HNT34847.1"
                     /translation="MVSIATPVLLLWLACSAIPCFANEKSESKGIHVICWNLSGHCDD
                     LKSTVEKMLDLDYVSGLLLYVDWRQFEPERGGYQWELFEIPLEAAEKRDKTISLALMV
                     CARSPDWVMQKTETFAFRHIHPSVGDVTAPVPWNADYTDALYKTVAAVGARLDGHKGL
                     RYVSINGPSTLFGVETNFPIASIAEHEANKVAFSLDKYERYWKESIDHFIDCFPKTPL
                     ALGLHDQISPKPRNSEQDVAVAKAIRNYAIEQERSKNGKRIVLRLLGLGSDNPKYFPG
                     PYQGDPSILNDYLSLVWEVREHVAVAFEATRVFRNSNAGDRKPLSAEDFGRVLQNGIS
                     YSPDWIEI"
     gene            1790..>2883
                     /locus_tag="PKH07_07610"
     CDS             1790..>2883
                     /locus_tag="PKH07_07610"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HNT34848.1"
                     /translation="MSQQKRTIITLAVLALTLLGSISASAAEFRGMWVTRFEWPNSNQ
                     TTAKAKIDTIMTNLKDHGFNAVLFQMRGQGDVLYPSPYEPWSALLGGLGQNPGWDPMQ
                     YAINSAHSRGLEFHVYINTHVVWSSSSQTPPAHTTPEHPYWLYYNPSGNHDWTICDSA
                     GTPVYYSSDNYCWCAPGVPSFQEWIRKVTMHVVNTYDVDGIHYDRIRVPANTYSYDPI
                     SNARRAGSGNPHSLDFKNWTRDQITRMLTDIYGSVMEVKPWVTMSCSPFGIYDRSRFA
                     GYSGFTDGYDRWLQESQHWLEVGCMDAVVPMIYWDIPDPAPNFDILVQDWIDYRYGRH
                     VYAGASAANYTFPELGNEITQTRTRGGQGFV"
BASE COUNT          659 a          728 c          791 g          705 t
ORIGIN      
        1 ttgatctcaa tccagtctgg actgtaggag ataccgttct gcagaactcg accgaaatcc
       61 tcggcgctga gaggctttcg atctccggcg ttgctgttcc tgaagactcg agttgcctcg
      121 aaagcaacgg caacgtgttc tctcacctcc cagaccagag agaggtaatc gttcagaatt
      181 gagggatctc cctgataggg gccagggaag tatttcggat tgtcggaacc caatcccagc
      241 agacgaagga caattcgttt gccattctta ctccgctcct gttcgattgc gtagttgcga
      301 attgccttcg ctacggcgac atcctgttcc gagtttcgtg gcttcggact gatctggtca
      361 tgaagtccca aggccagggg cgttttcggg aaacagtcta tgaagtgatc aattgattct
      421 ttccagtatc tttcgtattt gtcgagactg aatgcaacct tgttggcttc atgctctgca
      481 atggatgcga tggggaagtt cgtttccact ccgaagagtg tcgagggccc gttgatgctc
      541 acgtatcgca gtcccttgtg cccgtcgaga cgtgcgccaa ccgccgccac ggttttgtac
      601 agcgcgtcgg tgtagtcagc gttccaggga acgggagccg tgacgtcgcc aacactggga
      661 tggatgtgtc tgaaagcaaa cgtctctgtt ttctgcatga cccagtcggg agaacgagcg
      721 cagaccataa gagccaggga aatggtcttg tctcgcttct ctgcggcttc caagggtatc
      781 tcgaacagct cccactgata tccacccctt tctggttcga actgtctcca gtcaacgtag
      841 agaagcagtc cggagacata gtcaagatcg agcattttct cgacggttga cttcaggtca
      901 tcgcagtgtc cagagagatt ccaacaaatg acatggattc ctttgctctc tgatttctca
      961 ttggcaaagc acggaatcgc agagcacgcc agccacagga gcagaacggg agtcgcgatg
     1021 cttaccatgg tgacatggaa cctcctgctc ggtctgaggt gttcgagcat gaacaggata
     1081 tcctctcaag tgtctgagtg acaagggcaa aatctcaaca gcgtgggcaa agaacaacat
     1141 gggcgtatca gctcagttac gctggcgcta cagccaggat atgcttcgga agcaaagcgg
     1201 agtcttcgga gtgacgctac cgaagcctgc tgtcttgctg ttctcgcttc ggcagcgtcg
     1261 cagagcctct gcaaccgaag catacctcca ccccttccgt aactgatcta gtacacatga
     1321 gcaaagaaaa cgctttacat gaccaaaacg ggagtgtatt ctatctatat aaagtgttgt
     1381 gattgttgtc ttgaggctgt gccaccaggg caggctgatc gacagaatgt gttgggattg
     1441 agttgtgggg atgtgattat ccccttgagt tgaccgggta agctaattga atgcatcttt
     1501 tgtggtaaac agtcaagatg ggctggctct atgcggcagt tgttctgtag tatgcgtgtt
     1561 gcctatttgc tctgtcccat ctgaggcgtg ttcaccgggc gtggagacca gccaagcgtg
     1621 tggctgggat tccatcaaga gctgaaaggg ggagtgtgtc gcacggagtc ctgatgatgc
     1681 ggtgtattga agccctgagg aagttggact gaaatcctgt ctcttccttc ggggagggaa
     1741 acgagctttg tgctccagtg ttttgggaag caagcgaaag gaattattca tgagtcagca
     1801 gaagagaacc atcattactc tggcagtcct ggccttgacg cttctgggaa gcatctctgc
     1861 ttctgccgca gagtttcgag gcatgtgggt cactcggttt gaatggccga acagcaatca
     1921 aacaaccgcc aaagcgaaaa ttgacacgat tatgaccaac ctaaaagatc acggtttcaa
     1981 tgccgtgctc ttccagatga ggggacaggg cgacgtgctc tatccttcgc cctatgaacc
     2041 ttggtctgca ttgttgggag gactgggaca gaatcctggt tgggatccga tgcagtacgc
     2101 catcaactca gcgcactccc gtgggttgga gtttcatgtg tacatcaaca cgcatgtcgt
     2161 ctggtcttca agcagccaga caccaccggc acacacaacg ccggaacacc cctactggct
     2221 gtactacaat cccagtggga atcacgactg gacaatttgc gactcggcag gcactccggt
     2281 ctattattcc tcggataact attgctggtg cgctccaggg gtaccgtcgt tccaggagtg
     2341 gattcgcaag gtgaccatgc atgtggtcaa cacctatgac gtggacggga ttcattatga
     2401 ccggattcga gttccagcaa acacgtattc ctatgatccg atcagcaacg cccgacgtgc
     2461 cggctcaggc aacccccaca gcctggattt caagaactgg acacgggatc agattacccg
     2521 aatgctcacg gacatctatg gctctgtcat ggaggtgaag ccgtgggtca caatgtcctg
     2581 ctcgccattc ggtatctacg atcgaagtcg ttttgcaggg tacagcggat tcaccgatgg
     2641 ctatgaccgg tggcttcagg aatcccagca ctggcttgag gtcggttgca tggatgctgt
     2701 agttccgatg atctactggg atattcccga tccggctccg aactttgaca tcttggttca
     2761 ggactggatt gactatcgct atgggcgcca tgtctacgcg ggcgcatccg cagcaaacta
     2821 tacgtttccg gaacttggca atgaaatcac acaaacgcga actcgtggag gacagggctt
     2881 tgt
//
LOCUS       DAOQUC010000380         2870 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_40971_length_2870_cov_3.658615, whole genome shotgun sequence.
ACCESSION   DAOQUC010000380 DAOQUC010000000
VERSION     DAOQUC010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2870
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40971_length_2870_cov_3.658615"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(365..928)
                     /locus_tag="PKH07_07615"
     CDS             complement(365..928)
                     /locus_tag="PKH07_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HNT34849.1"
                     /translation="MKKVKGFTLIELLIVVAIIGILAAIAIPNFLAAQTRSKVARAEA
                     EHRQLATALETYYIDNNVYPPAGNWAAPGGQPFDTYGALMDVPYNITTPVAHLTSLPT
                     DPFRDNNTKSYMYGANAPNGLTCWMLTSYGPDNTKTGTIETPYLSDGTVPGSCDPSVR
                     ADRINPVNIYDPTNGTTSVGEIYRTGP"
     gene            1648..2799
                     /gene="dnaN"
                     /locus_tag="PKH07_07620"
     CDS             1648..2799
                     /gene="dnaN"
                     /locus_tag="PKH07_07620"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HNT34850.1"
                     /translation="MRFKIDRNKFQRGIQIVQNIPQSNLSNPILEHMYLAVDASGTVT
                     MRTSNLTLNAICRVEGTVMEPGEIAVPMKEMAQIVKELPIAEVMVEGDEGMIRIDCGP
                     ASLRLRGVNADEFPQVTFLENGVELELSASEFGGMVDKTIFATSEDRSRYILEGIKID
                     AEGQRLKFISTDGKRLSLVERAMEKELEKSVSVLVPGKTMGEIRRIITGEDRIRIKIG
                     EKKIEFFGDDFTLTSNLLVDNFPPYRQLIPSEFTLSFESGTADFLRSVRRASVLVDER
                     TLMVVLEVSPEGILVRGQSQAHGEANTIVDVEYDSEPFKTAFRCDFLQEVLKVLEGEK
                     VRFEFKNPTSPAVIKDSKDPGFLHVLMPLKVEENIAPAAEEQPEDEEEQ"
BASE COUNT          793 a          657 c          667 g          753 t
ORIGIN      
        1 gtagcctctc gagtcgctta tcaagtgtat ggagaaaaga agtatccgga aactgatccc
       61 aaagaacagg aagtggcttg tctcgaggcc gaatcttcag atcacaaatc cgcgcttgtg
      121 cctccctatc cgagagacga catccgatgc catacacggt atctgtcggg aaaatgatga
      181 tttctgacat ttctcccagc cctctcccat gaaactggac ttcaaagaag tctctttctt
      241 ttcatggaca agcactcaag gctgtgggca tagcccacgc gcttgcagtt tccaacctgc
      301 ttgaaagcaa aagaggggag agcgtttttc atctctcccc tccattagaa ttgttccgat
      361 gaaattacgg accagttctg taaatctcac caacactggt cgttccgttc gtcggatcgt
      421 aaatgttgac aggattgatt ctgtcagcac gtacggatgg atcgcaagag ccaggaaccg
      481 tgccatcact caaatacgga gtctcaattg tccccgtctt tgtgttatct gggccgtaac
      541 tggtcagcat ccagcatgtc aaaccgttgg gcgcattggc accatacata tatgactttg
      601 tgttgttgtc acggaatgga tccgtcggca acgaagtcaa gtgcgcgacc ggagtcgtga
      661 tattgtacgg aacatccatc aacgctccat aagtgtcaaa cggctgtcca cccggagcag
      721 cccaatttcc cgccggcgga tacacattat tatcaatgta ataagtctcc aacgcagtag
      781 ccagctgacg gtgttcagcc tcagcgcgag ccaccttgga acgagtctgc gccgccagga
      841 agttcggaat cgcaatagct gccaaaattc cgatgatcgc gacaacgatc aacaactcaa
      901 tcaacgtgaa accctttact ttcttcatta tttacatttc acctccttcc ctctaggtca
      961 ggaaacattg cgtatacaac gcaaaaatca ctcttcccaa gagcctccct ttgccctctg
     1021 cctgatagag tccacttcta tccaaggttc tctaccaaaa atcatactcc aatgtcaaga
     1081 aaattcttct ttttttttcg cttttgccac cctggacaac aatcataaga ccttatcttt
     1141 caccaacgtt aaatctatct tcaacccacc tcagcatcaa gacaaaattc agatgactta
     1201 ctcgatatcc gcttcacccg ctaagaagcc aatctggaca agaccacaaa gtgagtatcc
     1261 tgtgaagaaa tcgcttccaa aatcaaaaag caccatttcc agacaagaaa cctccgaatc
     1321 ctgtggaaag tctgaacaac ctcgtcttct tatccacagg tttttgaatt ccctaaaccg
     1381 ataaaaacta acagtctaga gcgaagttaa ctctatccac aggctctctt tctctattga
     1441 catctttaga ttgatcttag atactcattt gaattcagat ttgttcaaac agtgaaatcc
     1501 caagattggt cgttgggaat gggattcctg atacttggtc tgttctcgta gccaaaaatc
     1561 tgagtaagca ggaaatggta gtcgcctgac ccaggttttg tggtagagtc aaactggcta
     1621 tcagagcaat gaaaggagac tgcggatatg cgtttcaaga ttgataggaa taagtttcag
     1681 aggggaattc agattgttca aaacattccg caatcaaatc tctcaaatcc gattcttgaa
     1741 cacatgtatc tggcggttga tgcttctgga accgtcacaa tgcgtacaag caatctgacg
     1801 ctgaatgcaa tttgtcgtgt tgaaggaacg gtgatggagc caggagagat cgcagttccg
     1861 atgaaggaga tggcgcagat tgtgaaggaa ctgcccattg ccgaggtcat ggttgagggt
     1921 gatgaaggga tgatccgaat tgattgtggt ccggcatctc ttagattacg cggtgtcaac
     1981 gctgacgaat tccctcaggt gacgtttctt gaaaatgggg tggaactgga attgagtgcc
     2041 tctgagtttg gtggcatggt ggataagacc attttcgcca catcagagga tagatcgaga
     2101 tatatcctcg aagggatcaa gattgatgct gaaggccaac gattgaaatt catcagtaca
     2161 gatggaaaga ggctttcctt ggtcgaaaga gccatggaaa aggagttgga aaagtctgtt
     2221 agtgttctgg ttcccggaaa gacgatggga gagatccgga gaataattac aggtgaggat
     2281 agaatccgga ttaagatcgg ggaaaagaag atagagttct ttggtgatga tttcacgctg
     2341 acatctaatc tattggtgga taattttcca ccatatcgtc aactcatccc ctcagagttc
     2401 acgctttctt ttgagtctgg aactgctgat tttctgcgct ctgttcgccg tgcctcagtg
     2461 cttgttgatg agagaacgct tatggtggtt cttgaagtct caccagaagg aattcttgtt
     2521 cgaggtcaat cacaggctca tggtgaagcc aatacaattg ttgatgtcga gtacgattcc
     2581 gaacctttta agacggcatt tcgatgtgat tttttgcagg aagttctcaa ggttctcgaa
     2641 ggagagaaag ttcgttttga gttcaaaaat ccaacgagtc cggcggtcat taaggattca
     2701 aaagatcctg gattccttca tgtgttaatg ccactgaagg tggaggagaa tatagcgccg
     2761 gcagctgaag aacagcctga ggacgaagaa gagcagtagg tgtaatatcc caacggccta
     2821 tctacgatct gtggagtcct gacacctacg gagagggcga tagcccagcg
//
LOCUS       DAOQUC010000381         2870 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_40973_length_2870_cov_3.389343, whole genome shotgun sequence.
ACCESSION   DAOQUC010000381 DAOQUC010000000
VERSION     DAOQUC010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2870
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40973_length_2870_cov_3.389343"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..877)
                     /locus_tag="PKH07_07625"
     CDS             complement(<1..877)
                     /locus_tag="PKH07_07625"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HNT34851.1"
                     /translation="MNSRRALVSVVGLSVMCLMGGCLVLEQVEEILGGSISQTAAPEV
                     PREFRGVWVATVSRIDWPPEAGTEEQKKAMVDILDLAVEMNLNGVVFQVRPAADALYE
                     SKLEPWSRYLTGSQGKAPEPYYDPLAFAVEQAHKRGLELHAWFNPYRAGLSQNEEYAS
                     NSIVKARPELVRKHGSLKWMDPGEDDVKKQSLDVMLDVVKRYDIDAVHMDDYFYPYGA
                     GEGFDDDASWKKYKKSGGTLEKGDWRRKNVDDFIEKLSQEMRKIKPYVKLGISPFGIW
                     RPGYPEGISGMDQYNS"
     gene            complement(936..1925)
                     /locus_tag="PKH07_07630"
     CDS             complement(936..1925)
                     /locus_tag="PKH07_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNT34852.1"
                     /translation="MALKVGVVGVGNIGTLHAECYHGNPKAELVAVCDIVKEKADRAA
                     QRFGAKPFYSVKALLKSGIHLDACSMCTKGEENGGDHFTPTMELLEAGIPVLGEKPIS
                     NKVSEGKKMVALGKKMKIPYAVNLNHRFTPAALRAKEWIAEGRLGTLKTINMRMWINN
                     PVETSPWFHLRALHPHSFDVIRYFCGNVKSVAAFLNKGDGRITWSNAQVILHFENGVV
                     GHLLGSYDAGPMYGLEACDVTGSKGRFVIDDACEHLRFYSRSSIEMEQYSYLGGMRSF
                     GETFKSRIGRWVEQLESGVKPEDVDGSGEDALKAQLIIEAAIKSWQTHTIVDL"
     gene            2159..>2870
                     /locus_tag="PKH07_07635"
     CDS             2159..>2870
                     /locus_tag="PKH07_07635"
                     /inference="COORDINATES: protein motif:HMM:NF013595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34853.1"
                     /translation="MKRRLVPIVTLAVGLVIIAVVLLALNSQRQHSEQPHPVEEQPLE
                     PSRPWVYEPPDDEATLIFGQCYADQAIPNGLDARGLVQPNWVAVDTSVEPYRLYVVDA
                     QNFRVLGYKNPLAEPPVAADLVLCQTSEYTAYFFEEDPGRTQFWNISAVAVDSEGNVY
                     ITDSDHHRIVRFDRPFETDNLADAVYGQPSFDEGDPDYGTDRLAFPRGLWFDHQSDTL
                     WVADTGNNRVLAYINPLED"
BASE COUNT          655 a          830 c          697 g          688 t
ORIGIN      
        1 tcgagttgta ctgatccatt ccagagatac cctccggata gccgggtcgc cagattccaa
       61 agggactgat tcccagtttg acatagggct ttatctttct catctcctgg ctcagcttct
      121 cgatgaagtc atccacattc ttgcggcgcc aatctccctt ttccaaagtg cccccggatt
      181 ttttgtactt cttccagctg gcatcatcgt caaagccctc tcccgcacca tagggataga
      241 aatagtcgtc catatgcacc gcatcaatgt catagcgctt aaccacatcc agcatgacat
      301 ccagggattg cttcttcaca tcatcctcac ctggatccat ccatttcagg ctgccgtgct
      361 tgcgaacaag ttccggacgt gccttgacaa tactgttgga ggcgtattct tcgttctggg
      421 aaaggccggc gcgataggga ttgaaccagg catgaagttc caagcctcgc ttgtgcgcct
      481 gttcgacggc aaacgccaga gggtcatagt acggctcggg agcctttccc tggctccctg
      541 tcaaatagcg agaccacggc tccaacttgg attcatacag agcatccgcc gcgggacgaa
      601 cctggaacac gactccattg aggttcattt cgaccgcgag atcgaggata tcaaccattg
      661 ccttcttctg ctcttcggta cccgcttcag gtggccagtc aattcggctg accgtggcaa
      721 cccagactcc gcggaattcc ctggggactt cgggagcggc agtctgtgaa atggaaccac
      781 ccagaatctc ctccacttgt tcgagcacga gacaacctcc catcagacac atgaccgaca
      841 gcccgacaac cgaaaccaac gctctccttg agttcatgtc catgtcctcc cttggccgtc
      901 tacggccaga cccggatgct acggcgctcc aaatctcaca gatcaacgat cgtgtgcgtt
      961 tgccatgatt tgatcgcggc ttcgatgatc agctgcgcct tcaaagcatc ctcaccggaa
     1021 ccgtccacat cttcaggttt cacacccgac tccaactgct ctacccagcg accgatgcga
     1081 ctcttaaagg tctcgccaaa agaacgcatc ccgccaagat agctgtactg ttccatttcg
     1141 atactgcttc gagagtagaa acggagatgc tcacaggcat catcaataac aaatcggcct
     1201 ttcgatccag tgacgtcaca ggcctccaga ccatacatcg gtcccgcatc ataactaccc
     1261 agcaagtgac cgacaacgcc gttctcaaaa tgcagaatga cctgtgcatt tgaccaggtg
     1321 attcggccgt cgcctttgtt cagaaacgcc gcaacactct tgacgttgcc gcagaaatac
     1381 cgaatcacgt cgaacgaatg cgggtgcaag gctcgcaggt gaaaccatgg agaggtttcc
     1441 acgggattgt tgatccacat gcgcatgttg atcgtcttga gggttcccag acggccttcc
     1501 gcaatccatt ccttggcacg cagcgccgca ggcgtgaagc gatgattcag attgaccgca
     1561 tagggaatct tcatcttctt tcccaaagcg accatcttct tgccttcaga gactttgtta
     1621 gagatgggtt tctcgcccag aacggggatg cctgcttcca agagttccat cgtcggggtg
     1681 aagtgatcac caccattttc ttcgcctttg gtgcacatgc tgcatgcatc cagatgaatg
     1741 ccgctcttca gcagtgcttt gacactgtag aaaggcttgg caccgaatct ctgcgcagcc
     1801 cgatcggcct tctcctttac aatgtcgcag actgcaacca gttctgcctt tggattgccg
     1861 tgatagcatt ctgcgtgaag cgttccgatg tttccaactc cgacaactcc caccttaagc
     1921 gccatgttct tctcctgttc atagtgtccc catctcggtt gagtcgggga cgtctttttg
     1981 gtctcaacag atatcatgga tgatacggca gaccgacgga aagtgtccat tgaattgtgt
     2041 accgctcaga caatcccgca gttgaaaacg gaaacacaat gatgtaatcc tgttgaagac
     2101 tgaaatggac tttggttctg ctttgtggtg aattcgagaa aatgaggaca aaggactcat
     2161 gaaaagaaga cttgttccaa tcgtgacact cgccgttggc ctggtaataa tcgcggtcgt
     2221 tctgctagcg ttgaactctc agaggcagca ttccgagcag ccccatcctg tggaagagca
     2281 gcctcttgag ccctcccgtc cgtgggtcta tgaaccgccc gacgatgaag ccactctcat
     2341 tttcggtcaa tgctacgctg accaggccat cccaaacggt cttgacgcac ggggtctggt
     2401 gcaaccgaac tgggttgcgg tggacacgag tgttgagccc tatcggctgt atgttgtaga
     2461 cgcacaaaac ttccgagtgt tgggatacaa gaatcctctt gcggagccac cggttgcagc
     2521 ggaccttgtg ctctgtcaaa ccagtgagta cacggcgtat ttcttcgagg aagatccggg
     2581 gcgaacgcaa ttctggaaca tctccgcggt ggcggtggac tcagaaggca acgtctacat
     2641 caccgacagt gaccaccatc gcatcgtccg atttgatcgg ccttttgaga cagacaactt
     2701 agcggatgcc gtctacggtc agccttcctt tgatgagggt gatcctgact atggcaccga
     2761 ccgacttgct tttccgagag gactctggtt tgaccatcag tccgatacgc tctgggtggc
     2821 agataccgga aacaaccgag tgcttgccta catcaatcca ctggaagacg
//
LOCUS       DAOQUC010000382         2870 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_40974_length_2870_cov_3.326821, whole genome shotgun sequence.
ACCESSION   DAOQUC010000382 DAOQUC010000000
VERSION     DAOQUC010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2870
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40974_length_2870_cov_3.326821"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..791
                     /locus_tag="PKH07_07640"
     CDS             <1..791
                     /locus_tag="PKH07_07640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34854.1"
                     /translation="SEIQGVSLAQHLRGASSKESRKAVSENLDPETYHLRSIRGNRFK
                     YIELKPGGSVLYDLKGDPGEHSDVSSERIEVSEELRAELDRFVGGSLLGWHIRAGWMG
                     SAHRFSISVSAISGFRNTVASDNTSLEVLDNGTMLVWQGSAGPNASVSLRFDTEAETI
                     LLSLRKDDETIPPTEVFVGSLVRSPKHEELSGLISVGGFLDAHDASIPVGAHQEFENA
                     SVSGVLIWKSLGSAELNPLDAEPEIAENLRSLGYVGQVRQATRK"
     gene            936..2048
                     /locus_tag="PKH07_07645"
     CDS             936..2048
                     /locus_tag="PKH07_07645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34855.1"
                     /translation="MSSEKKNVNPENPLGPLGWSLIVFVPIVLFVLVIMRLTSQHSRP
                     ASERSKGAVVETAAVQPTKPRPRQPENPNVNKDIENQLVVQMTQIAMQQMAQACHPWV
                     EPDLSHLRSLRVRYAVSDDDSNVVHELRIQDSSVWLFTEQVVSDGSNLAAGAPFLGAL
                     GDGHVWFWEQPDSDAQLFSTVSEASLWGVLIQPVPLEILRLLVTDQGEFECLISGDAV
                     VEQKLCFDIEVNPKKEVFVASEPPLGLRERVLGSAWVVRKIKLVIERTTSLIRELTLI
                     GDEREMIVTFSDYGVVHGGSEAPTSVALKWRERGQDTLSIRCNYGWIEYSLWALDNAE
                     IRWPSDNRVQMVSVKIDTDTEELASLMRRLKAPAEN"
     gene            2346..>2870
                     /locus_tag="PKH07_07650"
     CDS             2346..>2870
                     /locus_tag="PKH07_07650"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HNT34856.1"
                     /translation="MLRFSKALLVVPPTGLYIREDRCQTPIEELVTVAPRPPIDLMYI
                     AAMLEREGVECRILDCPVEKKSWQDFETILTDFAPGALFICITTPTLSEDLLAASIAK
                     RFDKSILTVAKGPHFHFRDQATLLKHPDLDMVIRGEYEETAAELARDGDLDKIPGLTF
                     RSERNFVRTQERAFV"
BASE COUNT          657 a          633 c          824 g          756 t
ORIGIN      
        1 cttccgagat tcagggggtc agtttggcac agcatctcag gggtgcttcc agcaaagaga
       61 gtcggaaagc ggtctcggaa aacctggatc ctgagacgta tcatcttcgg agcattcgag
      121 gcaaccggtt taagtatatt gaattgaagc caggtggttc tgttctctac gatctcaagg
      181 gagatccagg tgaacattct gatgtttcat cagagaggat tgaggtttct gaggaacttc
      241 gcgcagaact cgataggttt gttggcggtt cacttttggg atggcacatc cgagcaggtt
      301 ggatgggatc cgcacatagg ttctccatat cagtctctgc catttctgga tttcggaata
      361 ctgttgcttc tgataacaca agccttgagg ttctggacaa cggaacaatg cttgtctggc
      421 agggaagtgc agggccaaac gcttccgttt ctctgaggtt tgatactgag gcagagacga
      481 ttctgctttc gctcaggaag gatgatgaaa ctattccccc gacagaggtg ttcgttggaa
      541 gtctcgtgag gagtccaaaa cacgaggaat tgtcggggct gatatctgtc ggtggatttc
      601 ttgacgccca tgatgccagc attcctgtcg gagcacatca ggagtttgag aatgccagtg
      661 tctccggggt cctgatatgg aaatccttgg ggagcgccga gttgaatccg ttggatgcag
      721 agcctgaaat tgccgagaat cttcgctctt tgggatacgt tggccaagtg aggcaagcaa
      781 ctcgtaagtg aggtttgcat ccgtttgatt tgttttctct tttctggtta ggcaatagtc
      841 atgggtctgg tgtgaaaatg agacattctt tgtttgaaca tctctcggac aacgtgtagt
      901 cttttcctgc aaatacgcat tttggaggaa cggagatgag ttctgagaag aagaatgtga
      961 atcctgaaaa ccctcttggc cctctgggat ggagtctcat tgtctttgtc ccgatcgtcc
     1021 ttttcgttct tgtcatcatg aggctgacaa gccagcattc ccggccagcg agcgaacgct
     1081 cgaagggtgc ggtggtggag acagcagcgg tgcagccgac caagcctcgt cccaggcagc
     1141 ctgagaaccc gaatgtcaac aaggatatcg agaaccagtt ggttgtgcag atgactcaga
     1201 ttgccatgca acagatggcg caggcctgcc atccgtgggt ggaacctgat ctgtcacact
     1261 tgagatctct tcgggttcgc tatgctgtct ctgatgatga ttccaacgtg gttcacgagc
     1321 ttcggattca agactccagt gtatggttgt ttactgagca agtggtcagt gacggttcga
     1381 atcttgcggc gggtgctcct tttcttgggg cacttggtga cgggcatgtg tggttctggg
     1441 aacaaccgga ctctgacgct caactcttca gcacagtctc cgaagcgtct ctttggggcg
     1501 ttctgattca gcccgtgccg ttggagatat tgcggctact ggtcacggat cagggtgagt
     1561 ttgagtgcct cataagcgga gatgccgttg ttgagcagaa actatgcttt gatatagagg
     1621 tgaatccgaa gaaggaagtc tttgttgctt ccgaacctcc tctggggctt cgggagagag
     1681 ttctgggatc tgcctgggtt gttcggaaga tcaagttggt cattgagaga acgacaagcc
     1741 tcatcaggga gttgacgctc attggtgatg aaagagagat gattgtcact ttctcggact
     1801 acggtgtcgt acatggtggc agtgaggcac ctacttccgt ggcgctgaaa tggcgtgaga
     1861 gaggacagga tactctttcg attcgctgta actatgggtg gatagaatac tcgttgtggg
     1921 cgttggataa cgccgaaatc cggtggcctt cggacaaccg agtccagatg gtcagcgtga
     1981 agattgatac agatacagag gaactggctt cgttgatgcg tcgcctcaag gcaccggcgg
     2041 agaattagga aatttgacgc actcaagcct ggaattttgt tggcatccac atcagggtgt
     2101 ttcatgcgat cacatcttaa atccgatgct cttccttgca tccttgcaag gaatccgata
     2161 ctgtttcttg cagtcagccg tgaagttcga gagtcttgct ggttgaggaa ttgattcagt
     2221 cttcatattg gctgtcgaga aactagactt tggagaattc tggtttcccc tcaaaatgag
     2281 cacgtgcggg attgggcaga gcccaagcgc ctgtgagtgc agggatgttc ggaagggaag
     2341 caaaggtgct caggttcagc aaagcactgc tggttgttcc accaacagga ttgtacattc
     2401 gcgaagaccg atgccagacg cctattgaag aactggtcac ggttgccccg cgtcccccca
     2461 ttgacttgat gtatattgcg gcaatgctcg agcgagaagg cgttgaatgt cgcatacttg
     2521 actgtccagt agagaagaaa agctggcagg atttcgagac aattctcacc gactttgcac
     2581 caggggcatt gtttatctgt attaccactc cgacgttgtc tgaggattta cttgctgcct
     2641 caatagccaa gaggtttgac aagagcattc tcacggtagc taaaggtcca cactttcatt
     2701 ttcgggatca agcgaccctc ctcaagcatc ctgacttgga tatggtcatt cggggagaat
     2761 atgaggaaac cgcagccgag ctcgcacggg atggggattt ggataagata cccggcctca
     2821 catttcggtc agaacggaat tttgtgcgaa cgcaagaacg cgcctttgtg
//
LOCUS       DAOQUC010000383         2869 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_40999_length_2869_cov_3.749822, whole genome shotgun sequence.
ACCESSION   DAOQUC010000383 DAOQUC010000000
VERSION     DAOQUC010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2869
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40999_length_2869_cov_3.749822"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..391)
                     /locus_tag="PKH07_07655"
     CDS             complement(<1..391)
                     /locus_tag="PKH07_07655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34857.1"
                     /translation="MKIKTRKKQHGRLSKTLILLLLLVTAFFVLPNVLIAPRLREKLE
                     KKMNRAGSLQGSMGSLTINLFTQRISFGNVSVNYLEKAYSASAKVPSIKVRSRTLPLL
                     RGEIVPRSIVITKPVIVVEEPGVPLPRG"
     gene            505..1854
                     /locus_tag="PKH07_07660"
     CDS             505..1854
                     /locus_tag="PKH07_07660"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNT34858.1"
                     /translation="MVLLLGTGPLPLYTHDRLYAFGVRTWHFARCLLDEDIPTVLVTF
                     EFGRKGSEVLSINSSDIPERLKHVALPEPDGTNLEFLVEQIKQLIAQYQPTCMVTAGS
                     NLPAQIAVRCGSEIPLWVDLFGDMMTEVQAKLAFAPASEMDFFLRLYHSLLFRGDRFS
                     TVSEAQKDAVIGQLSMTGRLTGATRDCDLIHVIPVSFDDRTPPPVVREPLLRGRRTPE
                     NAFIILWSGGFNTWADIPTLFTGLECAMSANSDIHFAASGGSITGHHEAGFRQFLDLV
                     QTSKHKERFHILGWLPNEAVAGLYAESDLGINVDLDIYEARLGSRCRIVNWMNAGLPC
                     LTTRTTELSKILEANQIGFVFPPSSPEALSEAILHLASNRQGIRDTGTRARVYAEEHF
                     AFGRTIRPLLEWVSNPVVAPDRVQESHSPLMRSLHDLLTNASAPQAPRTSFWKWLRR"
     gene            complement(2306..>2869)
                     /locus_tag="PKH07_07665"
     CDS             complement(2306..>2869)
                     /locus_tag="PKH07_07665"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNT34859.1"
                     /translation="ERGELDLVRTRNGLLPKLDMFIALGGTTYASSFSPGDDRDGQGW
                     DASLGLKFQYNLGNRAELAQHRRTTLNQLQVEQSLRNMEQTVQVDVRSAHVKVQVAAE
                     KIRATEATRKLREESSRIEAEKFRVGRSTEILVAQAWRDLVTAEIAEISAVIAYRKAL
                     VDLYRLEGSLLERRGVIVSEPGESLGN"
BASE COUNT          687 a          779 c          738 g          665 t
ORIGIN      
        1 cccccctggg taagggaact ccaggctctt caacgacaat tacgggcttt gtgatgacga
       61 tggagcgagg aacgatctcc cctcgcaata aggggagagt ccgagacctg actttgatcg
      121 aaggaacctt tgcagatgca gaataggcct tctccagata gttcactgat acgtttccaa
      181 aggaaatcct ctgtgtgaaa aggttgatcg tcaggctacc catcgagcct tgaagtgaac
      241 ccgcgcgatt catcttcttc tccaacttct ccctcaagcg cggggcgatc agcacattgg
      301 gaaggacaaa gaatgctgtc accagcagca agaggagaat cagcgttttg gaaagccttc
      361 cgtgttgttt cttgcgggtc ttgattttca tagattacct ctaaaatcca ttaacacatt
      421 gtctttcgcc cgaacatact ttattcttat caacaagaat gcctatcgga cagagagtct
      481 atcaggaaag cggactgaga caaaatggtg ttgcttctcg gaacaggccc actgccgctg
      541 tacacacatg atcggctgta cgcctttggg gtgagaacct ggcacttcgc tcggtgtctt
      601 ctcgatgagg atatcccgac agtgctggtg accttcgagt tcggaagaaa aggttccgaa
      661 gtgctgtcca tcaactccag cgacattccc gagcgactga agcatgtggc tcttcccgaa
      721 ccagacggaa cgaatctgga gtttctggtg gaacagatca agcagctcat tgcccagtac
      781 cagcccacct gtatggttac agcaggctcg aatcttcccg cgcagattgc tgtccgatgc
      841 gggtcggaga tccctctctg ggtggatctc ttcggggaca tgatgactga ggtgcaggca
      901 aagttggcct ttgcccctgc atctgaaatg gatttcttcc tgcgccttta tcacagtttg
      961 ctgtttcgtg gagaccgatt ctcgaccgtt tcggaggcac agaaagatgc cgtgatcggt
     1021 caactgagca tgacagggcg cttgacggga gcgacacgag attgtgactt gatacacgtt
     1081 attcctgtga gttttgatga caggacacca ccccctgtag ttcgagaacc attgcttcgg
     1141 ggcagacgta cgccagagaa tgcgttcatc attctttgga gtggtggttt caacacttgg
     1201 gcggacattc cgacactgtt tacagggttg gagtgcgcca tgagtgccaa ctccgacatt
     1261 cacttcgccg cgtccggagg ctcgattacc ggtcaccatg aagcgggctt cagacagttt
     1321 ctcgatcttg ttcagaccag caaacacaag gagcgttttc acatccttgg atggctgccc
     1381 aatgaagccg ttgccggact ctacgcggaa tccgatcttg ggatcaacgt tgatctggat
     1441 atctacgaag cgcgcttggg gagccgttgc cgcatcgtca actggatgaa tgccggcttg
     1501 ccctgtctga ccacccgaac cacagagttg tcaaagatcc ttgaggccaa tcagatcgga
     1561 tttgtatttc caccctcaag cccagaggca ctttcggaag cgattcttca cctggcgtcc
     1621 aaccgccagg gcattcggga cacaggaaca cgcgccagag tctatgcgga ggaacacttt
     1681 gcctttggta ggacgattcg gcctttgctg gaatgggtca gcaatcctgt cgtggctcca
     1741 gacagagttc aggagtcaca ctcgccgctc atgagatcgc tgcatgatct cctcacaaac
     1801 gcttctgcac cacaggctcc gcgaacttct ttctggaagt ggctcaggcg atagcctcaa
     1861 ccgtgcgttg ttcttctgaa ccgttgtaat caaaagaaaa atcaggagag aaaacaggcc
     1921 accgttcttt gggggaacgg tggccgattg ccctgggtgt gtctgggaat gctgcgggga
     1981 acgatggtgc gaggcacagg acaaccgggc caccgttgaa acgtagactg ccgaaaagga
     2041 cgaaagttac ctaaccggca gagtcattac atcgcggagt gagaaactca acacgaatgc
     2101 cgaaagtgaa gcggtcacct ccaaaaccac atgaaaccca agttccctta aaaaatccaa
     2161 tcacaataca aagacagaat caattgcttc aacgttatta tgattgccac aggaagatgc
     2221 aaaaagcaag gcttttttcc gctttttttg cctgtagatc acgtttgctc gccaacggtt
     2281 gccaaagaag cacggccaga gcaaatcagt tccccagaga ctctccgggc tccgaaacta
     2341 tgacaccgcg tctctccaag agagagcctt caagccggta gagatccaca agcgctttcc
     2401 gatacgctat gaccgctgag atttcagcga tttccgctgt gaccaagtcc cgccatgcct
     2461 gggcaacaag gatttccgtc gagcgaccta cacggaattt ctccgcctca attcggctgc
     2521 tctcttctcg gagttttcga gttgcctcag tagctctgat cttctccgca gcgacctgaa
     2581 ccttcacatg ggcgctacga acgtctacct gcaccgtttg ctccatgttg cgcagcgact
     2641 gctccacctg aagctggttc agagtcgttc gacgatgctg cgccaactcg gctcggttgc
     2701 ccaggttgta ctgaaacttc aggccaagag aagcatccca gccctgccca tctcgatcat
     2761 cacccggaga aaaagagctg gcataggtgg ttccacccag ggcgataaac atgtccagtt
     2821 tgggaagcaa tccgttgcgg gttcgaacga gatccagttc cccacgctc
//
LOCUS       DAOQUC010000384         2869 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_41004_length_2869_cov_2.831912, whole genome shotgun sequence.
ACCESSION   DAOQUC010000384 DAOQUC010000000
VERSION     DAOQUC010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2869
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41004_length_2869_cov_2.831912"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1607)
                     /locus_tag="PKH07_07670"
     CDS             complement(<1..1607)
                     /locus_tag="PKH07_07670"
                     /inference="COORDINATES: protein motif:HMM:NF022623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HNT34860.1"
                     /translation="MSHSTFLVFALSLLSLVSSAGGRELTYEELVYRMIDLEKISVLP
                     EDGENCAQWSSYDRRSQYDSESDRYVEWSANGDGDGFIREEDGVQVLAEMDGPGCIWR
                     IWSARADSGHMKIYLDDDTAPVLDRPFQEFFDPDNGVFPFSNLVHTTAKGKNCYIPIP
                     YQKSCKIVAEAGWGKYFQFTYSTFPKGTTVPSFSQPLLEQNRQALANVDCCLGTKSGK
                     NPVTQYTDAKTLTRKVSAAPGETVRIARLRGPRAIVFLNLNIHLKDRDEEIQALRELV
                     LQIRWDDEEQPSVWTPLGDFFGTAPGINPYLSLPLGMNEQGFYSLWYMPFRKSAKVEI
                     INEGSSPRSLDVTISHSRLNRPVAEYGRFHAKWHRDVLEPAEPERWPDWTLLKTTGRG
                     RYCGAHLHVWSPRKGGCKEFAGEGHWWWGEGDEKFFVDGEKFPSTFGTGTEDYFGYAW
                     CDPQRFENAFHNQSLNEGNAGHISVNRWHIADNVPFQESFEASLEKYFPNRWPTVYAA
                     TVYWYLAPDGTDPYLPVHVWERLGHWM"
     gene            complement(1684..>2869)
                     /locus_tag="PKH07_07675"
     CDS             complement(1684..>2869)
                     /locus_tag="PKH07_07675"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNT34861.1"
                     /translation="SLTIDSTASLSLRDTTRRRGRSLATIALIAFGSFVVVAVGANRR
                     GSVEDFLDRSSGTGGFAVYAESALSVTQHPANILEQVGLSEGVSAELTVLPMRVRDGE
                     DASCLNLNRAQSPRIIGVNPDPLSSRHSFSFTKTVANSDQFSGWNLLKRDQPNAVVPA
                     IGDEATVIWGLGKSVGDKLTYLDDQGGAFDIVIVGLVKRSILQGSLFIAEDAFLEHFP
                     SESGYRAFLFDCPLERRTESISMLSQAFQDAGMEVTTTESRLSRFDTVENTYLEIFLT
                     LGGLGVILGISGLGVVVMRNAFERRSEFALLQAVGFPRRTIRFMVLCEHAFLISYGLL
                     CGTLSGLVALIPRFTQVAPEFPWLILGLHLSAMFSCGILCAWFASAFVCSGSILDALR
                     GE"
BASE COUNT          715 a          757 c          752 g          645 t
ORIGIN      
        1 tgcatccagt ggccaagtct ctcccaaacg tgaacgggca ggtatggatc tgttccatct
       61 ggagcgaggt accaatagac tgtggcggcg taaaccgtcg gccagcggtt tgggaaatac
      121 ttctcaaggg atgcttcaaa ggactcctga aaaggtacat tatctgcgat atgccaacgg
      181 tttaccgaga tgtgaccggc atttccctca ttgagcgact ggttgtggaa tgcgttctca
      241 aatctttgag ggtcacacca ggcatagccg aagtaatcct ctgttccggt tccaaatgtg
      301 gatggaaact tctctccatc cacgaagaat ttctcgtctc cttcacccca ccaccaatga
      361 ccttcacctg cgaactcctt gcagccgcct tttcggggac tccaaacgtg aaggtgcgcg
      421 ccgcaataac ggcctcggcc tgttgtcttc agcagtgtcc agtctggcca gcgctctggc
      481 tcggcgggct ctaagacgtc acgatgccac ttcgcgtgaa aacggccgta ctccgcaacg
      541 ggtctgttga gcctcgaatg actgatggta acatccaatg atcttggaga tgacccttcg
      601 ttgatgatct cgactttagc gctcttgcga aaaggcatat accagagcga gtaaaatccc
      661 tgctcgttca ttccaagggg aagcgacaga tagggattga ttcctggagc ggttccaaag
      721 aagtcaccca agggagtcca tacgctgggt tgctcctcgt catcccagcg aatttggaga
      781 accaattccc taagcgcttg aatctcctca tcgcgatctt tgaggtgaat gttcagatta
      841 aggaacacaa tggctcgggg gccacgcagt cgggcaatcc tgacagtctc gcccggagct
      901 gccgagactt tccttgtcaa cgtctttgcg tctgtatatt gggtgactgg attcttcccg
      961 gatttggtgc caaggcagca atccacattt gcgagtgcct ggcgattctg ttcgagcaaa
     1021 ggttgcgaga aggaaggaac agtagttcct ttgggaaaag ttgagtatgt gaactgaaag
     1081 tacttgcccc aacccgcttc cgcaacgatc ttgcaggatt tctggtaggg aatcggaatg
     1141 tagcagttct tgcccttggc ggtcgtgtgt accagattgg agaaggggaa gacgccgttg
     1201 tcaggatcga agaattcttg aaaaggcctg tcgaggaccg gtgcggtgtc atcgtcaagg
     1261 tagatcttca tgtgacccga gtctgctcgt gctgaccaga ttcgccaaat acagccggga
     1321 ccgtccatct cagccagaac ctgtacgccg tcttcctctc ggatgaatcc atccccgtcg
     1381 ccatttgcag accattcgac atatcggtct gactctgaat cgtattgact tcgacggtca
     1441 tagcttgacc actgagcgca gttctcccca tcttcgggca gcacggaaat cttttccaaa
     1501 tcaatcattc gatagacaag ctcctcatag gtcagttctc ggccacctgc acttgacacc
     1561 agagacaaca gactcagcgc aaagaccaga aatgtggaat gtgacatgaa gacctccgtg
     1621 gattccaaat cggcgtatca tccactctgg ggacgataac atcccgtaga agacgcagca
     1681 attctattct cctcgaagag catccaggat ggagccgctg cagacaaaag ccgaagcgaa
     1741 ccaggcacag agaattccgc aggagaacat ggcagacaag tgcagtccga gaatcagcca
     1801 ggggaattct ggtgcgactt gtgtgaatct ggggatcaga gcaaccaagc ctgacaatgt
     1861 tccacagaga aggccatagg aaatcaggaa tgcgtgctca cacagcacca tgaatcgtat
     1921 tgtccgtctt gggaatccta ccgcctgaag aagcgcgaat tcactgcggc gctcgaaagc
     1981 gtttcgcatg acgacgacgc ccaatccgga tattcccagt atgacgccga gtcccccaag
     2041 ggtcaggaat atctcgaggt aggtattctc cacagtgtca aatctcgaca atcggctctc
     2101 agtagtggta acttccatgc cagcatcctg aaaggcctga ctgagcatcg agatggactc
     2161 agttcgcctt tccagtgggc agtcaaacag gaaagctcga tacccactct cagaaggaaa
     2221 atgctccagg aatgcgtcct ctgcgatgaa cagactgcct tgcaggatgg agcgtttgac
     2281 cagcccaacg atgacgatat cgaaagcccc tccttggtcg tccaggtaag tcaacttgtc
     2341 accgaccgat ttcccgagac cccagatcac agtggcctca tctcctattg caggaacaac
     2401 ggcatttggc tgatcacgtt tgagcaggtt ccagccactg aattggtcag aattcgcaac
     2461 tgtcttggtg aaggagaacg aatggcgtga agaaagaggg tcaggattca caccgatgat
     2521 ccgaggtgac tgcgctcgat tcagattgag acaacttgca tcctctccgt cacgaacgcg
     2581 catgggaagg actgtcagtt cagcagaaac accttcggag agtccaactt gttcgagaat
     2641 gtttgccgga tgttgtgtga cagaaagggc ggactctgca tagacggcaa aacctccagt
     2701 accagaactc ctgtcgagaa agtcttcgac ggagcctcgt cgattggctc caacggcgac
     2761 cacaacaaag cttccaaagg caatgagggc tatggttgca agacttcgtc ctcggcgtcg
     2821 ggttgtatcg cgcagagaca atgaagcagt tgagtctatc gtgaggctg
//
LOCUS       DAOQUC010000385         2861 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_41189_length_2861_cov_2.598004, whole genome shotgun sequence.
ACCESSION   DAOQUC010000385 DAOQUC010000000
VERSION     DAOQUC010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2861
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41189_length_2861_cov_2.598004"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2861
                     /locus_tag="PKH07_07680"
     CDS             <1..>2861
                     /locus_tag="PKH07_07680"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HNT34862.1"
                     /translation="TFTIEVFPADHYLSYVTPSHGGNSGSTHIQVRGLRFVPGMHVKI
                     RNQLGDIEAESGVADLEETLLGLSLNLTGMSPGVRSIVVRWPSGTEQMLENSFQIVPG
                     VGPKFEARLILPETTRQGRKYVLWAEYENKGDSDMPSPLLALATTSDVSMSFSPEGPF
                     LRNPLLFLAVNFSDPAGILPPGSKNRIPLYFIATDPCQFSFGTVGFFGVLDTEFSSME
                     DYFLAMSNAATRLTRRGDGIKYKLDDLDPFERDRRNGISVGAISGTVFNEYTQSQAWN
                     TKVQAIPDGEVAQVTGESVTNDLGHFVMERLEPGGFDFHVPGYFMPSAVEVELATDED
                     VNDVDLYIWPLATPTPDPCDGWWGAPPVGDASAQSVMVLGTHCVDGSFSSESLTSKLK
                     WLKLTGADKGFKVAARLQGCGEINGCELSISGGLSGAVDTPFEGFEISGSGKGKWKVD
                     PEKCEWDFKSGNATLSGQVTKKYPIGMKFLPPGMKFLDIAKLDLAVSASASGSAAWKE
                     GDYFPGLPSDGSVSLGVGFSAIGSVRSYTGSPKGEVSVTGSVNATLGFGNTPSSVVGC
                     ATLKASGEIGKIRYNYTLQECEHFVGGSPASYSSDIVPEIEIISYIGLPTRFGSNTVL
                     ASVESDLQEDGAPSLAVSPGGVVLCVWTKDRPESNLLGSAVVASEYSGGTWTTPTTIA
                     SYDRICTNAAVVYDSSGEAWAVWNSAPTVTQGSSAEEISEALNSSTIYYSHRVAGTWS
                     TPAALGDLEGLDKMPTVAAGDNGLLCAAWVCSASENSSIVASLYDGSQWSQPEVLSSG
                     PASEQPTVVFDGSIPCVVWSEEAALCMARTASSVLMSSEYRSSAWSFPQPVHNEPRET
                     QSFPTMAIDDNGIKYLAWQESGLIHCATGQNGNWLASYTISDSTGSSPKMALCGNKPF
                     IIYETGFGNDADIVYIMGELSGSSFLWSDKETLSPTTAY"
BASE COUNT          650 a          708 c          816 g          687 t
ORIGIN      
        1 cgactttcac catagaggtc ttccctgccg accattatct ttcctatgtg actccatctc
       61 acggcgggaa ttccggctca acacacatcc aagttcgagg gcttcgtttt gttcccggaa
      121 tgcatgtaaa gattcgcaat caactgggcg atatcgaagc ggaatcgggt gtggctgatc
      181 tggaggaaac gttgcttggg ttgtccctta atctgacagg aatgtctccg ggggtacgca
      241 gcattgttgt gcgctggcct tcgggaacag agcagatgct agagaattct ttccagatag
      301 tgcctggagt cggaccgaaa ttcgaggctc gcctgattct tccagagaca acgagacagg
      361 ggagaaagta tgtgctctgg gctgaatacg agaacaaagg tgacagtgac atgccatcgc
      421 cacttctcgc tcttgcaacc acttcggacg tctcaatgtc attttctcca gagggacctt
      481 ttctgcgtaa tcctctgctg ttcttggcgg tcaatttcag cgatccagcc ggcatcttgc
      541 ctccgggttc aaagaatagg attcccctct atttcattgc gacggatccg tgccaattct
      601 ccttcggaac agtgggtttc tttggagtgc tggacaccga attctcgtcc atggaagact
      661 acttcctggc aatgagtaat gcagcaacac gcctgacaag gcgcggtgac gggatcaagt
      721 acaagttgga cgatctggat ccatttgagc gcgacaggcg aaacggcatc tccgttgggg
      781 caatatccgg aaccgtgttc aacgaatata cgcagtcgca ggcttggaat accaaggttc
      841 aggcaatccc ggatggagaa gtcgcgcaag tgaccggaga atctgtaacg aacgatctgg
      901 ggcactttgt gatggagcgt ttggagccag gaggatttga ttttcacgtt ccagggtact
      961 tcatgccttc tgcggtcgag gtggaactgg caactgacga ggacgtcaat gatgtagatc
     1021 tgtacatctg gccactcgca acgccaactc cagatccgtg cgacgggtgg tggggagctc
     1081 cgcctgtggg agatgcctcc gctcagtcgg tcatggttct gggaacccat tgtgttgatg
     1141 gctctttttc ttcggagtcc ctgacctcaa agttgaagtg gttgaaactt accggtgcgg
     1201 acaagggttt caaagtggcg gcgcgactcc aaggctgtgg ggagatcaat ggttgcgaac
     1261 tctcaatcag cggaggtttg agtggagcag tggacacacc gttcgaaggt ttcgagattt
     1321 ctggttcagg caaaggcaaa tggaaagttg accctgagaa atgtgaatgg gatttcaagt
     1381 caggaaatgc cactctttct ggccaagtca cgaaaaaata cccgataggc atgaaatttc
     1441 tgccaccggg aatgaagttc ctggatatcg caaagttaga tcttgcggtc tccgcctcag
     1501 cgtcaggaag cgcagcgtgg aaagaaggag actattttcc cggtttgcct tccgacggtt
     1561 ccgtttcctt gggagtgggc ttctcagcaa ttggttctgt gaggtcctac accggctcgc
     1621 caaagggcga agtgagcgtc acgggttcgg tcaatgcaac gcttggtttt ggcaacactc
     1681 cgagcagtgt tgtagggtgt gccacgttaa aagcttcggg tgaaattggc aagatacggt
     1741 acaattacac cctgcaagaa tgcgagcatt tcgttggagg ttcacccgcc tcgtatagca
     1801 gtgatattgt tccggagata gagatcatct cgtatatagg tctaccaacg agattcggtt
     1861 ccaacacggt gctggcgagt gtggagtccg atcttcagga ggatggtgcg ccaagtctgg
     1921 cggtcagtcc tggaggcgta gtcctgtgtg tttggacaaa ggaccgcccc gaaagcaatt
     1981 tgttagggag tgctgtggtt gcatcagagt actctggtgg gacttggacg accccaacaa
     2041 ccattgcgtc ctatgacaga atttgcacta acgctgccgt ggtctatgac agcagtggcg
     2101 aggcttgggc tgtctggaat agtgcaccaa cagtcactca ggggagttcc gcggaagaga
     2161 tatccgaagc actcaacagc agcaccatct actactcgca ccgagtggca ggcacctggt
     2221 caacgccagc cgcgttgggt gacttggaag ggcttgacaa gatgcccacg gtggcagcag
     2281 gagacaacgg gcttctctgc gctgcctggg tctgttctgc ttcggagaac agcagcattg
     2341 ttgcctcgct ctacgatggc agccaatgga gccaacccga agtcctgtct tccggacccg
     2401 catccgagca gcctacagtt gtcttcgatg gttccatccc ttgtgttgtg tggtctgaag
     2461 aggctgcact gtgcatggcg agaaccgcct cgtccgtgtt gatgagttca gagtatcgtt
     2521 cttctgcgtg gtcgttccca caaccggttc acaatgaacc cagagagacg caaagcttcc
     2581 caacaatggc cattgatgac aacggcatca agtatcttgc gtggcaggaa tctggcctga
     2641 tccactgcgc tacgggacag aacgggaatt ggcttgcttc ttacacgata tctgattcga
     2701 ccggatccag ccccaagatg gccctgtgtg gcaacaagcc cttcatcatc tatgagacag
     2761 gctttggaaa cgacgctgac atagtctaca tcatgggcga gctaagcggt tcgtcgttct
     2821 tgtggtcaga caaggagact ctctctccga caacagcgta t
//
LOCUS       DAOQUC010000386         2858 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_41252_length_2858_cov_2.975740, whole genome shotgun sequence.
ACCESSION   DAOQUC010000386 DAOQUC010000000
VERSION     DAOQUC010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2858
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41252_length_2858_cov_2.975740"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1557
                     /gene="mutL"
                     /locus_tag="PKH07_07685"
     CDS             <1..1557
                     /gene="mutL"
                     /locus_tag="PKH07_07685"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HNT34863.1"
                     /translation="ALGSRLTISEDGTQWSQAGCPEGTTVEVADLFYNVPARRKFLKS
                     TQTEYNAVAETLVRQALAFPSVHFVLTHNGRKTLEFPGVDSREQRVLQVLGKSAAEHF
                     MPVAGLSGDFSLEGFISLATEHRKNAAQIYFLVNDRYVQDRLMLRALLDPYRTLLPSG
                     RYPFGVLYLAMPPDTVDVNVHPAKMEIRFHDENSVFHLIRSTVFRSLNEFTGQTLAVQ
                     TPQNSTARATPEIPRQTSMFPQSQHTSSTKRERDLDEVIQSAVSAFGDRLAAQERAET
                     LGSIQGQSVPTGTAPSASTTTHDGFTTPVSEQKPVPAPVTPQILFDEHRRFVPRMQLN
                     NVYMVGYVNGEPDLIIIDQHAAHERILFEQFRASRAGHCMPSQNLLIPLTWDVAPSDV
                     DIVCSHLDALRTLGFEIEYFGGETFNVTAIPATLDLERSAQLLQDCISDLAQGQKDHS
                     AAEDLENALLERLACHAAVRAGQALSLEEMESLGLQLCRTPNGGTCPHGRPTTLRLSE
                     SDLKKHFKRD"
     gene            complement(1629..>2858)
                     /locus_tag="PKH07_07690"
     CDS             complement(1629..>2858)
                     /locus_tag="PKH07_07690"
                     /inference="COORDINATES: protein motif:HMM:NF013056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HNT34864.1"
                     /translation="IFVQPVILQNAGMDFGAVMTATCIAGALGTFLMAFLANYPIALA
                     PAMGHNLFFAFVICGAMGFTWQQALGANFISGCLFILLAAFKFRERIIEAVPDSLKNA
                     IAAGIGLLIALVGLGYGGLVVKSPGFLLEMGNLRSGAALLTLFGVGLIAVLLVLRVQG
                     AILWGILICALIGVPVGLVQKPDSIISSPPSLMPTLFKMDVMGALNKNLIAVIFVLFF
                     LDLFDTVGTLIGVAERGGMMKDGTLPRAGRALLSDAIATVAGTCLGTSTVTSYVESAA
                     GVAEGGRTGLANVVTGILFLLALFFSPLVRIVGGGYMPEGASMALYPAIAPALIIVGS
                     FMIGCVGRVNWDDYSEAIPAFLTVIVMGVTMNITEGIAFGFISYVTLKTVRGEGCKVH
                     PLTYIFAVLFLVRYMFL"
BASE COUNT          722 a          801 c          745 g          590 t
ORIGIN      
        1 gcgctcggca gtcgcctgac catctccgaa gacgggacac aatggtcgca agcaggctgt
       61 cccgagggaa cgacagtcga agtggccgat ttgttttaca atgttcccgc acgacgcaag
      121 tttctcaaga gcactcagac tgagtacaac gcggttgctg aaacgctggt caggcaggcg
      181 cttgcttttc ccagtgtgca ttttgtgctc acccacaatg gtcgaaaaac attggagttt
      241 cccggggtgg attctcgcga gcaacgggtg cttcaggttc tcggaaaaag tgcagcagaa
      301 cacttcatgc ctgtcgcagg tctttccgga gacttctcgc tggagggttt catctctctg
      361 gcgacagagc atcgaaagaa cgcagcgcag atctatttcc tggtcaatga ccgatacgtt
      421 caagaccgtt tgatgttgcg tgccctcttg gatccgtacc gaacgctgct tccgtcgggt
      481 cgctatcctt tcggcgtgtt gtatctcgcg atgccccccg acacggtgga tgtcaatgtc
      541 catcccgcca agatggagat tcgttttcat gatgagaaca gcgtttttca tctgatacgc
      601 tcaacggtgt ttcgcagtct gaacgagttc acgggacaaa cgcttgcggt gcaaacaccg
      661 cagaattcga cagcaagggc gactcctgaa atcccgcgac agacttcgat gtttccacag
      721 tcacagcaca cctcaagcac aaaacgggaa cgcgatctgg acgaagtcat ccaatcggcg
      781 gtcagcgcat tcggtgatcg gttggctgca caggagagag cagaaacgct cgggtctatt
      841 cagggtcagt cagtgccgac cggtacggct ccgagtgcct cgacaacgac ccacgatgga
      901 tttactacgc cagtatctga gcagaaaccc gttcctgctc cagtaactcc gcagattctt
      961 tttgacgaac accgcaggtt cgtgccgcgg atgcagctga acaatgtcta tatggtcggc
     1021 tatgtcaatg gtgagccgga tttgattatc attgaccaac atgcggctca tgaacgcatc
     1081 ctcttcgagc agtttcgcgc ttcacgagca ggacactgta tgccctcaca gaatctcttg
     1141 atcccattga cgtgggacgt tgctccttcg gatgtggaca ttgtctgttc gcatctggat
     1201 gctcttcgta ccctgggttt cgagattgag tatttcggcg gagagacctt caacgtcaca
     1261 gccatccccg caacactgga tctggagcga agtgcgcagt tgcttcaaga ctgcatctcc
     1321 gatctggcgc agggacagaa agatcattct gcggcagagg atcttgagaa tgcgctgctg
     1381 gaacgtctgg catgccatgc ggcggtgcgc gcaggacagg ctctgtcgct tgaggagatg
     1441 gaatcgcttg ggcttcagct gtgtcgtacg cccaatggcg gaacctgtcc ccatggaaga
     1501 cccaccacac ttcgtctgag tgaaagcgat ctgaagaagc acttcaaacg agactgacca
     1561 cagtcgaatg tcggcaacaa tcacgagagc agatgagagg attcatttcc gcagaccgat
     1621 gagtttttct acagaaacat atatcgaacg agaaacagca cggcaaatat gtacgtcagt
     1681 ggatgaacct tgcatccttc tccccgaact gttttcagcg tcacgtaact gatgaatccg
     1741 aaggcgatac cctccgtgat gttcattgta actcccatga cgatcacagt gagaaaagca
     1801 ggaatggctt ccgaataatc atcccagttc actctgccga cacatccaat cataaagctg
     1861 ccgacaatga tcaaggctgg cgcgatggcg ggatagagtg ccatggacgc accttccggc
     1921 atatacccac caccaacaat tcgaaccaaa ggcgagaaga acagcgccaa cagaaaaagg
     1981 ataccagtca ctacattggc aagccctgtt ctgcctcctt cggcaacacc tgccgcgctc
     2041 tcgacgtagc tggtcacagt cgaagtaccc agacaggtgc cggcaaccgt cgcgatggcg
     2101 tctgaaagaa gcgccctgcc cgcacgcggc aacgtgccat ctttcatcat gccaccacgt
     2161 tcggcgactc caatcaacgt tcccaccgtg tcaaagagat cgaggaagaa caggacaaag
     2221 atcaccgcaa taaggttctt gttcaaggct cccatcacat ccattttgaa gagcgtcggc
     2281 atcaaggacg ggggtgagga aatgatcgaa tccggtttct gaaccagtcc gacgggaacc
     2341 ccaatgagcg cgcagataag aataccccaa agaatagccc cttgaacacg aagcaccaag
     2401 agcactgcaa tcagaccgac gccgaaaagc gtaagcagcg ccgctccact tcgcaggtta
     2461 cccatctcca aaagaaaacc gggactcttc actaccagtc caccgtagcc aagtccgaca
     2521 agggctatca acagaccgat gcctgctgcg atggcgttct tcagggagtc aggaaccgct
     2581 tcgataatcc tctcgcgaaa cttgaacgcc gccagcagaa tgaagagaca gccagaaatg
     2641 aagttggcgc ccaaggcctg ttgccaggtg aaccccatcg ctccgcagat cacgaaagca
     2701 aagaacagat tgtgccccat cgccggagcc agtgctattg gatagttggc cagaaaagcc
     2761 ataagaaacg ttccgagcgc accggcgatg caggtggcgg tcatcaccgc gccgaagtcc
     2821 atgcccgcgt tttgcaggat gacgggttgc acaaagat
//
LOCUS       DAOQUC010000387         2854 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_41344_length_2854_cov_2.941408, whole genome shotgun sequence.
ACCESSION   DAOQUC010000387 DAOQUC010000000
VERSION     DAOQUC010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2854)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2854)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2854
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41344_length_2854_cov_2.941408"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            8..859
                     /gene="nadC"
                     /locus_tag="PKH07_07695"
     CDS             8..859
                     /gene="nadC"
                     /locus_tag="PKH07_07695"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003613566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HNT34865.1"
                     /translation="MNLKIDAKIRSRIKAALQEDLGKSGDITSLWTIPDQMHGKAEII
                     SRTSGVVCGVTVVEKVFACLDKSVETDFSVSDGDRVKSEQVVATISGSMRSILAGERT
                     ALNYLTHLSGIASLTRRFVDLASPFGVSVLDTRKTTPLLRTLEKHAVVCGGGKNHRMG
                     LYDMVLIKDNHIAAVGGVRQAIRAAKASRENEDIQIEVEVSTIQEARIAAEEGVDIIL
                     LDNMTPAIVRDAVEVIAGMAEVEVSGGITLENVHDYAITGIDRVSIGSLTHSVHALDM
                     SLEIVQN"
     gene            905..1990
                     /gene="thiE"
                     /locus_tag="PKH07_07700"
     CDS             905..1990
                     /gene="thiE"
                     /locus_tag="PKH07_07700"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HNT34866.1"
                     /translation="MRPMDEGIYRMIDANLHRAAEGLRVLMDIARFVCDDVGASGRLK
                     AVRHGLFEAVETLPSYYAKAVQARDAEEDVGRSAGAGVMGRRHDLPDLYLANLHRSQE
                     ALRALEECAKLISPVLGSQLEDLRFTLYSVDKAFLPSVLKAGSESLMDFDLYVVTDQS
                     LSRGRDFVTVIESAVQGGAGAIQLRAKHLGKREMLSVARDIRSLTRERGITFFVNDHL
                     DIALACEADGCHLGQDDFPIDEARRIVGAGFILGASTHSVEQALEAEAQGASYVNVGP
                     IYPTKTKETSCPPVGVELITKVKERVRIPQTCMGGINSSNVHEVILAGADRVAVVSAV
                     VAADDIEAAARNLLDRISQAKKQRGDD"
     gene            1990..>2854
                     /locus_tag="PKH07_07705"
     CDS             1990..>2854
                     /locus_tag="PKH07_07705"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: protein motif:HMM:NF014067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HNT34867.1"
                     /translation="MTLLEEARQGKVTEEIERVAALEHVDSEWLRNRVASGKVVIPAN
                     RHRRLEKICGIGAGLKTKVNANIGSSPFNPCPEAEERKLRVAEDAGADTVMDLSTGGD
                     LNEMRQHVARTTRIPLGTVPIYQYMVDYKNSCKPDLGNLMLEYLDQHGKDGVDFVTVH
                     CGVTRQAIESIEEERLTGIVSRGGAFLARWMREMNEENPLYSRFDEVCDVLAQHEMTL
                     SLGDGLRSGCIHDANDRAMIHEMLTLGALARRARAKGVQVMIEGPGHMAVNQIEANVR
                     LQKQVCDGAPYY"
BASE COUNT          648 a          679 c          873 g          654 t
ORIGIN      
        1 tgtgagaatg aacctgaaga ttgacgcgaa gatccgaagt cgtatcaagg ctgctttgca
       61 ggaagatctc ggcaaatccg gagacatcac atccctatgg acgattcccg atcagatgca
      121 cggcaaagca gagatcatct ccaggacttc tggagtggtc tgcggtgtga cagtggtaga
      181 gaaggtattt gcctgcttgg acaagagcgt tgagacagat ttttcggttt ccgatggcga
      241 tcgggtcaag tcggagcagg tggtcgcaac catctctggt tccatgcgtt cgattctcgc
      301 gggggagcgg actgcgctga actatttgac tcatctgtcg ggaattgctt ctttgacgcg
      361 gcgatttgtg gatctggcct cacctttcgg tgtttcggtc ttggacacgc gcaagaccac
      421 gccccttttg cggacacttg aaaagcacgc ggttgtgtgt gggggtggga aaaatcaccg
      481 catggggctc tatgatatgg ttctcatcaa ggataatcac atcgccgctg tcggaggagt
      541 gcgccaggcg attcgagctg ccaaagcgtc ccgcgagaat gaggacatcc agatcgaggt
      601 tgaggtcagc accatccagg aagctcgaat cgctgccgaa gaaggcgttg acatcattct
      661 gctggacaac atgactccgg ccattgttcg agatgccgtc gaggtcattg ccggaatggc
      721 ggaagtcgag gtttctggtg gcattacact cgagaatgtc catgactatg ccatcacggg
      781 catagacagg gtttcaatcg gatctctgac gcactcggta catgctctgg acatgtcact
      841 ggagatagtt cagaactgac atctgtcagg cgctgtttgg tgcctgagtg ccaaggaaat
      901 atacatgaga ccgatggacg aaggcatata ccgaatgatt gacgcgaatc tgcaccgcgc
      961 tgccgaaggg ttgcgtgtgc tgatggatat tgcccgattt gtctgtgatg acgtcggtgc
     1021 gtccggtcgg ctcaaggcag tccgccatgg gcttttcgag gctgtcgaga ctctgccctc
     1081 gtactatgcc aaggctgtcc aggcgcgcga tgcggaagag gacgtagggc gttctgcggg
     1141 tgcaggcgtc atgggacgcc gccacgatct ccctgatctg taccttgcca atctgcaccg
     1201 cagccaggaa gcacttcggg cgctggaaga atgtgcgaaa ctgatctctc cggtcctggg
     1261 aagccaactg gaagatctcc gattcacact ctactcagtg gacaaggctt ttctgcccag
     1321 tgttctcaaa gcaggttctg aatcgctgat ggattttgat ctgtatgtgg tgaccgatca
     1381 gagtttgagc cgcggtcgag atttcgtcac agtgatcgag agtgctgttc agggtggtgc
     1441 cggcgcaata caactgcgtg cgaagcactt gggaaagcgc gaaatgctgt ctgttgccag
     1501 agatatcaga tcgctgacac gggagcgtgg cattacattc tttgtcaacg atcatctgga
     1561 cattgccctt gcatgtgagg cggacggctg tcatctgggt caggacgatt tcccgatcga
     1621 tgaagccaga cggatcgtcg gtgccggatt catcctggga gcgtccactc acagcgtgga
     1681 gcaggcgctt gaagccgagg cgcaaggcgc ttcctatgtc aacgtcggtc ccatttatcc
     1741 aaccaagacc aaagagactt cctgcccacc ggtaggggtc gaactgatta cgaaggtcaa
     1801 agaacgggtg agaatacctc agacttgcat ggggggcatc aattcgtcta atgttcacga
     1861 ggtcatattg gcaggtgctg accgggtggc tgtggtttcg gcagttgttg ccgcggacga
     1921 cattgaagca gccgcccgta atcttttgga tcggatcagc caggccaaaa aacagcgagg
     1981 cgatgattga tgactttatt ggaagaggca cgacagggta aggttactga ggaaatcgag
     2041 cgggttgccg ctttggagca tgtggattcg gaatggctca gaaaccgggt tgcatcaggc
     2101 aaggtggtca ttcctgcgaa tcggcatcgg cgtcttgaga agatctgcgg catcggtgcc
     2161 ggactcaaga ccaaagtcaa tgccaatata ggatcttctc cattcaaccc ctgtcctgag
     2221 gccgaagaac ggaaacttcg cgttgccgaa gacgccggtg cggacacagt catggatctg
     2281 tccacgggtg gagatctgaa tgagatgagg cagcacgtcg ccaggactac gcgcatccct
     2341 cttggcacgg tgccaatcta tcagtacatg gttgactata agaacagttg caagccagat
     2401 ttgggcaacc tgatgctgga gtatctggat cagcatggga aagatggggt ggatttcgtc
     2461 acggttcatt gtggagttac gcggcaggcg attgagagca tcgaagaaga acgactgacc
     2521 ggcattgtca gtcgcggtgg tgcctttctg gcgcgttgga tgcgggagat gaacgaggag
     2581 aatcctcttt actcgcgttt cgatgaagtg tgtgacgtat tggcgcagca tgagatgact
     2641 ctgagtcttg gagatggtct gcgctccggc tgcattcacg atgccaatga tcgagccatg
     2701 attcatgaaa tgctcactct gggagcattg gcaagaaggg ctcgtgccaa aggggttcag
     2761 gtcatgatag agggtccggg tcacatggcc gtcaatcaaa ttgaggcaaa cgtcagactc
     2821 cagaaacagg tctgcgatgg tgcaccctat tacg
//
LOCUS       DAOQUC010000388         2852 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_41380_length_2852_cov_3.269575, whole genome shotgun sequence.
ACCESSION   DAOQUC010000388 DAOQUC010000000
VERSION     DAOQUC010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2852
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41380_length_2852_cov_3.269575"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="PKH07_07710"
     CDS             complement(<1..210)
                     /locus_tag="PKH07_07710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34868.1"
                     /translation="MKKAVPYPSEILKGEKSQRCFSGANAIQVAMPIGGIGAGCICLN
                     GSGGFQDFSIRGKPLTSAAPDGHRYI"
     gene            423..1292
                     /locus_tag="PKH07_07715"
     CDS             423..1292
                     /locus_tag="PKH07_07715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34869.1"
                     /translation="MQTWIWRSLRIDLPDDWEMLQFSRNPERGRCAFADRYQFRLELS
                     WRKVGSSPDFPQMIKDYESQLRQESDRSVIRRISHGMWSGVDCDSKAMLSSRLGRYFE
                     AESCLVEIVLIWPDVRDSALMCQILSSIDAYSASNEGLQRWKAFGIDLWAPKSFALQK
                     CTVEPARAVLSFTDSHRLQNQYFERLGMVSHWLKVPVSQWLREQSPKGIRISAETAVL
                     SSQHSIETITGIGRLRMVLPCFGKRLHYASVAWICPKDGRLYRVCSHSFAPIAPEVLA
                     SRLSCCTEKEKSK"
     gene            1289..1693
                     /locus_tag="PKH07_07720"
     CDS             1289..1693
                     /locus_tag="PKH07_07720"
                     /inference="COORDINATES: protein motif:HMM:NF017239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HNT34870.1"
                     /translation="MKARPSRNLTKSQSSWLAMLRAIPVQNTAAHVSESEQDEVCIIV
                     KIKPIPFLFAPFSWLIRPRRERRFRLEALGSRVWHLCDGERTVEEIVDIFAIQFGLTF
                     HEARVAVTRYMKTLVQHGALAVAVDNAQEVGA"
     gene            1690..>2852
                     /locus_tag="PKH07_07725"
     CDS             1690..>2852
                     /locus_tag="PKH07_07725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34871.1"
                     /translation="MTLRAVVIGLLGVSVICGFSYFNDNILQQTLSVGNSMPATVYGG
                     LLLFMLFINPLLRKFKQRYAIRGSELAVALTLTLAACVVPESGLLRTFTASLIMPHHY
                     YRTEPGWKEQKVIEIVPSHMLVDPSEDESLILNGFIQGLSSGKRHISPAKVPWHAWIT
                     PLAFWIPLVLALWVAVIALSVVLHKQWSAHEQLPYPIATFANSLLPREGETTPPIFRS
                     RSFWIATGVIFLMNFNNYLCQWFPDKMINIPNTFSLVPLRTLFPTFIAGGGERLLTVT
                     LYMTPIAVAYFLPTDVSFSLGLGPFLWFISIGVFAKYGISLTGTVEGASWYTSLKPQT
                     FLLFGANLGVFLTILYTGRHYYLSIVRQSFVPFRKSTTDMDAVWGFRVFAIALA"
BASE COUNT          639 a          768 c          730 g          715 t
ORIGIN      
        1 aatgtaccgg tgaccgtcag gagctgcgct tgtcagaggt ttgcccctga tggaaaaatc
       61 ctgaaaccca ccagaaccgt tcagacagat gcagcccgcg ccaatgccac cgataggcat
      121 ggcaacctga atggcattgg cacctgaaaa acacctttgg ctcttctcgc ctttcagaat
      181 ctcagaagga taggggacag cctttttcat gaactcacct ctcgccgtac tccgagtaac
      241 ttccatcctt gtcgcaagga caaatcctca tcagaattcc agcaatcgta gcacaatcga
      301 agacctttgt ggatgaaatc cgcgatccct tcaatgacaa ccctgtcatt gagccttgcc
      361 ttcacccaaa cgggtggcta tagtccgaag gaagtctgag ttcacgtggg caagaggacg
      421 caatgcagac atggatttgg cgcagtctcc gaattgacct tccagacgat tgggagatgc
      481 tgcaattctc taggaatccc gaacgcggtc gctgtgcctt cgccgatcgg taccagttcc
      541 gtctggaact gagttggaga aaagtcggct catctccaga ctttcctcag atgatcaagg
      601 actatgagag ccagctgagg caggagtccg acagatcagt gatcagaaga atttcgcacg
      661 gcatgtggtc aggtgttgac tgtgactcaa aggcaatgct aagttcgcgt ctgggtcgct
      721 acttcgaggc agagtcttgt cttgttgaaa tcgttcttat ctggccggat gtcagagatt
      781 cggctttgat gtgccaaatc ctttcaagca ttgacgccta ctctgcgtca aatgagggat
      841 tacagagatg gaaggccttt gggatagact tgtgggctcc gaagagtttt gctcttcaga
      901 agtgtacagt ggagcccgcc agagcagttc tctcattcac ggactcccat cggctgcaaa
      961 atcagtattt tgaacgtctt ggtatggtct cgcattggct gaaagtgccc gtcagccaat
     1021 ggctcaggga gcaatcccca aaggggatca ggatttctgc agagaccgct gtgctgtcga
     1081 gtcaacactc gatcgagaca ataacaggta tcggaaggtt gagaatggtt ttgccctgct
     1141 ttggaaagcg cttacactat gcatcggtgg cctggatctg ccccaaggac gggcggctct
     1201 acagagtctg cagccattcg tttgccccga ttgcgccaga ggttctggct tcacgactct
     1261 cttgctgcac tgagaaggag aagtccaagt gaaggcaaga ccctcaagaa acctcacaaa
     1321 aagccagtct tcctggttgg cgatgctcag agccattcct gttcagaaca ccgcggcaca
     1381 tgtgtcggag tcagagcagg acgaagtttg tatcatcgtg aagatcaagc ctattccgtt
     1441 tctcttcgcc ccgttctcat ggctcattcg tcccagaaga gaaaggcgat tccgcctgga
     1501 agccctgggt tcacgagtct ggcatctttg tgacggggag aggactgtcg aggagatcgt
     1561 ggatatcttc gccatccagt ttggactgac ctttcatgag gctcgcgtgg cggtcactcg
     1621 atacatgaag acacttgtcc aacacggtgc gcttgccgtc gcggtagaca atgcccagga
     1681 ggtcggagcg tgaccctcag ggctgttgta atcggcttgc ttggtgtctc tgttatctgt
     1741 ggattcagct attttaatga caacattctg cagcagaccc tctcagtcgg caacagcatg
     1801 ccagcaactg tctatggtgg cctgcttctg ttcatgctgt tcatcaatcc tctgcttcga
     1861 aagttcaaac agaggtacgc aatccgaggc agtgagttgg cagtcgctct aacattgacg
     1921 ctggccgcgt gcgtcgtacc cgaatcggga cttctccgaa ctttcaccgc atcgctgatc
     1981 atgccacatc actattaccg aaccgagccg ggatggaagg agcagaaggt catcgagatc
     2041 gtgcccagtc acatgctcgt agatcccagc gaagacgaga gtctgattct gaatggtttc
     2101 atccagggac tcagttcggg aaaacgacac atctcccccg caaaagtgcc ctggcacgcc
     2161 tggatcactc ctcttgcgtt ctggattcct cttgtgctcg cgctgtgggt ggccgtcatt
     2221 gctctttccg tggttttgca caagcagtgg tctgctcacg aacagctgcc ctatccgatt
     2281 gccacattcg cgaattctct tctccccaga gaaggtgaaa ccacaccgcc aattttcaga
     2341 agtcgttcat tctggattgc caccggcgtt attttcctga tgaacttcaa caactaccta
     2401 tgccaatggt tccctgacaa gatgatcaac atcccgaata ccttcagtct cgtcccactc
     2461 aggacactct tcccgacgtt cattgccggt ggcggagagc gactgctgac ggtcacactc
     2521 tacatgaccc ccatcgccgt ggcttacttc cttccaacag atgtttcctt ttctctcggt
     2581 ttgggaccgt tcctttggtt catatccatt ggcgtttttg caaagtatgg catctcactg
     2641 acggggactg tggaaggagc aagttggtat acctctctga agccgcagac cttcctgctc
     2701 tttggcgcca atctcggtgt gtttctaacg atcctctaca caggaaggca ttactatctg
     2761 agcatagtgc gtcagtcctt tgttcctttc agaaagagca ccaccgacat ggatgccgtg
     2821 tggggtttcc gggtgttcgc gattgcactc gc
//
LOCUS       DAOQUC010000389         2852 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_41384_length_2852_cov_2.782982, whole genome shotgun sequence.
ACCESSION   DAOQUC010000389 DAOQUC010000000
VERSION     DAOQUC010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2852
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41384_length_2852_cov_2.782982"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1384
                     /locus_tag="PKH07_07730"
     CDS             <1..1384
                     /locus_tag="PKH07_07730"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNT34872.1"
                     /translation="YRSTSLLSLDRTGCCEAMQPKIKTEMQAIREGLKCSNLEAVFAT
                     VHVVLTQGVFLTNYVLDLKSSNMVCGIIESLPSLVQFSYFLSPILVRRLKYRRPIAVF
                     FFTAHRLAWLLLIALMYVDCSNRCKQVLLVLTLLGANCCAVIAGNAWFSWMTDLVPPA
                     IRGSYYGRRNVYLGITSMTSLFIGTQLLTFFRRVDMARSGYTLCFSVAIASALFSARI
                     MARQYEPKMKPVPKMSLVRLFQTVRHNRPLKDFIRFFIIWQFSMGISSAFFGVQMVRL
                     LKMTPAQMGYQTLISSVAALVGSRFWGRAMDEIGERAVTLASGFLIAMHIWFWLPARE
                     GFLQPIWVTSVLGGFSWAGFNIAVFSWLQRICGEEERQYTYGLLGAFAGPGFMLGSLA
                     GGLLTTVVPETLFSINAFAVTHFHLVFVLSSVGRMLAIFWIAAKSMGYGRDHRTILRC
                     LVDSALSLRR"
     gene            complement(1387..2121)
                     /locus_tag="PKH07_07735"
     CDS             complement(1387..2121)
                     /locus_tag="PKH07_07735"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HNT34873.1"
                     /translation="MLFPTAPHARRISSKWKSRGGQKLGRADTGNWLRGTNNRLYHVP
                     LYDSEEEVLHGVFLAVLDPVNMRIKETYRAEKGFYREGRWVFENCLHSVMGNGKIVRQ
                     ERFPTREMDLDEEPEDFAGVQLSAHEMGYFQLRDLIRDLGLTGGILKRYQAEMSYKLA
                     LPVSCFVLTLVGMAWSLKPHFSGISVELLGSILIGAGYMAANAFCLSLGGKGVLSPWI
                     AGWITTLLLAFIGFVLLIRRQYFAFA"
     gene            complement(2039..2479)
                     /locus_tag="PKH07_07740"
                     /pseudo
     CDS             complement(2039..2479)
                     /locus_tag="PKH07_07740"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
     gene            complement(2486..>2852)
                     /locus_tag="PKH07_07745"
     CDS             complement(2486..>2852)
                     /locus_tag="PKH07_07745"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HNT34874.1"
                     /translation="GLSRTELRERRDALLQSPKLEKEASKTLARIETELNNRIALPFA
                     CLVLAALGAPLGFWMQRGSRGACFALALVVILTYYFLMGFGTSLVEREVLSIQMGAWV
                     PNVIMGILGIMANVHVARR"
BASE COUNT          632 a          776 c          746 g          698 t
ORIGIN      
        1 ttacagatcc accagccttc tttcgcttga cagaaccgga tgttgtgagg ctatgcaacc
       61 aaagataaag actgaaatgc aggccattcg agaggggctg aagtgttcca acctcgaggc
      121 cgtctttgcc acagttcatg ttgttctgac acagggtgtc tttctcacca actacgtgct
      181 tgatctgaag tcctcaaaca tggtctgtgg gatcattgag tcacttcctt ccctggtgca
      241 gttcagttac ttcctgtccc ctatactggt gcggcggttg aagtaccgca gacccatcgc
      301 cgtgtttttc ttcactgccc accgactcgc ctggctgttg ctgatcgctt tgatgtatgt
      361 ggactgctcg aaccgatgca aacaagtcct tctggttctg acgctgttgg gggcaaactg
      421 ctgtgccgtg atcgctggca atgcctggtt ttcatggatg acggatctgg ttccaccggc
      481 cattcgaggc tcctactacg gtcgcagaaa tgtctacctc ggcatcacct ccatgaccag
      541 cctgttcatt ggtacacagc tgctgacctt cttccggcgg gtggatatgg cgcgcagcgg
      601 atacactttg tgtttctctg ttgccattgc ctcggcactg ttttccgcaa ggatcatggc
      661 gaggcagtac gagccgaaaa tgaagcccgt tccgaagatg tcgctggtaa gactgtttca
      721 gacggttcga cacaatcgcc cacttaagga tttcatccga ttcttcatca tctggcagtt
      781 ctcaatgggc atcagttcgg cgttctttgg ggttcagatg gtgcgtctgc tgaagatgac
      841 gcccgctcag atgggctatc agactttgat ctcaagcgtg gccgcacttg tgggcagtag
      901 gttctgggga cgcgccatgg acgagatcgg agagcgcgct gtgacactcg catcgggttt
      961 tctgatcgcg atgcatattt ggttctggtt gcccgctcgt gaaggatttc tgcagccgat
     1021 ctgggtcacc agcgtcttgg gtgggttctc ttgggcgggt ttcaacatcg ccgtcttttc
     1081 atggcttcaa cgcatctgcg gagaagagga acgacaatat acctatggtc ttctgggagc
     1141 gtttgccggc cctggattca tgcttggatc gcttgcgggt ggactgctga cgacggtcgt
     1201 tcctgagaca ctgttctcga ttaacgcctt tgcagtcacg catttccatc tggtctttgt
     1261 tctttcttcg gtggggcgaa tgcttgctat cttctggatc gctgccaagt ccatgggata
     1321 cggtcgtgat caccgcacga tccttcgctg tcttgtggac tcagcgctct ctctccgacg
     1381 ttgattctag gcaaaggcga agtactgtct tcgaatcaag agaacaaatc cgataaaggc
     1441 gagcaacaga gtggtgatcc agccggcaat ccagggactg agcactccct tgccacccag
     1501 actgagacag aaggcgttgg cggccatgta gccggcacca atcagaatac ttccgagcag
     1561 ttccaccgat attcctgaga aatgcggttt gagtgaccaa gccattccaa ccagagtcag
     1621 aacaaagcag gatacaggca gcgccagttt gtaggacatc tctgcctggt atcgtttgag
     1681 aatgcctccg gtaagtccca gatcacggat gagatcgcgc aactggaagt accccatctc
     1741 atgggcactc agctggactc ccgcaaaatc ctcgggttcc tcgtccagat ccatctcacg
     1801 cgtgggaaac ctctcttgtc gaacgatctt gccatttccc atcacgctat ggaggcagtt
     1861 ttcaaagacc catcttccct cgcggtagaa gcctttctcg gcgcgatatg tttctttgat
     1921 cctcatgttg accggatcca ggacggcaag aaagacgcca tgcagcactt cctcttccga
     1981 atcgtagagt ggcacatggt agagtcggtt gttggtgccc cgaagccagt tgcccgtatc
     2041 ggcgcgaccg agtttctgcc ccccccttga tttccacttc gaggatatcc tgcgagcgtg
     2101 cggtgcagta ggaaacaaca tactcctgaa catagaagaa caggatactg atcaagacgg
     2161 acaagaccaa tgttggtgcc atcagtctca cgggagagaa tccggacacc agacactcca
     2221 tcagttcgtt gcggctgttc attcgatgca ggaaatagaa cgatgccaga agcatgacca
     2281 aaggcatgat ttccgccacc gacagaggga cgtagaaaag aatgtatttg accgaaagcc
     2341 ccagaggtgc ttcgtttttg agaagtgttt cgaattcctt gagcgccacc gacacgccca
     2401 tgatggtggt cacacccacc aggacggaca acagtacgat gacatactcc ctcaggagat
     2461 atcgatccag tgtattcatt ctaagttacc ttcgagccac gtggacattt gccatgatac
     2521 ccagaattcc catgatgaca ttgggcaccc aggctcccat ttgaatgctc aggacttcgc
     2581 gctccaccag agaggttcca aagcccatca gaaagtagta tgtcaggatc accacaagag
     2641 ccagagcaaa acaggcaccg cggctgccac gctgcatcca aaaacccagg ggcgctccaa
     2701 gggctgcaag cacgagacag gcaaagggaa gggcaattcg attgttgagt tctgtctcga
     2761 tccgcgccag agtcttggat gcttctttct caagttttgg cgactgcaaa agagcatcac
     2821 gcctttcccg cagctccgtt cgggacagtc cc
//
LOCUS       DAOQUC010000390         2851 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_41413_length_2851_cov_2.887339, whole genome shotgun sequence.
ACCESSION   DAOQUC010000390 DAOQUC010000000
VERSION     DAOQUC010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2851
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41413_length_2851_cov_2.887339"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            204..1037
                     /gene="kdsA"
                     /locus_tag="PKH07_07750"
     CDS             204..1037
                     /gene="kdsA"
                     /locus_tag="PKH07_07750"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007958967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HNT34875.1"
                     /translation="MASNNMKIGAIQVDSGGPLFVIAGLCVLESEALSLSVAHTLKEI
                     CSRYELPYLFKASFDKANRSSLRSSRGPGIEKGLEILKRIKEECEVPVVTDVHEASQV
                     MPVAEVVDVLQIPAFLCRQTDLLIAAAETGRTVSVKKGQFLAPWEMKNVVEKIESVGN
                     HNILLVERGTTFGYNNLVVDFRSIPMMRSLGYPVVFDCTHSLQMPGSLGERTGGQPEF
                     IPTLARAAVAAGADGVFLETHPSPRDALSDGANCLILTQFESLIESLAALSRVRKQFS
                     V"
     gene            1081..>2851
                     /gene="gyrA"
                     /locus_tag="PKH07_07755"
     CDS             1081..>2851
                     /gene="gyrA"
                     /locus_tag="PKH07_07755"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013118934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HNT34876.1"
                     /translation="MFAQNERIRPVNIEEEMKVSYISYAMSVIVSRALPDVRDGLKPV
                     HRRVIFSMHELGLGPSRPYRKSAKIVGETMGKYHPHGDSAIYDSLVRMAQDFSIRYPV
                     VDGQGNFGSVDGDPPAAMRYTEARLSRVGEAMLTDIDKETVDFIPNFDESEMEPTVLP
                     SVIPNLLVNGSAGIAVGMATNIPPHNLVEVVDAVLAQIDNPDITTRELMALLPGPDFP
                     TGATICGRQGILDAYETGRGRVVQRATAVLEQSKAGKQNLIVTEIPYMVNKANLIREM
                     ADLIRDKRIEGIADLRDESDRDGMRIVIELKRGENYEVVLNQLYKHTKMQNTFGVILL
                     ALVDGRPVYLSLKEIIDQFIKHRREVITRRTRFLLAKAEARAHILEGLRIALDNIDAV
                     VALIRKSADAEQAKTGLMNKFKLSDKQAQAILDMRLQRLTGLEREKLEEEYRALLKDI
                     EYYKNVLSNAALLTEIMKTELREIKERFGDDRRTKIVEDETDLDIEDLIAEENMVVTV
                     SHQGYIKRISTDTYRQQKRGGRGIIAVETKETDFVEQMFIASTHHYVLFFTNQGRVYW
                     QKVYALPQGGRATRGKAIVNLLEL"
BASE COUNT          674 a          678 c          816 g          683 t
ORIGIN      
        1 ctagcaatcg ctccgatgtc tataatccgt caaagatact agaccgcaat tgtgttcatt
       61 tcaacgcagt acaggtggcc gttttcagca tgagttatcc ttgactgaat agggctgctc
      121 tgatatagtt cctgtagtgt atgattcttc gcataagtgt ttgagatctc acaacgtaga
      181 gtcgcgagga gtgttttgtc agcatggctt cgaacaatat gaagataggg gccatccagg
      241 ttgattctgg tggccctcta ttcgtgattg cgggcctctg tgttctggaa tccgaggcac
      301 tatcgctttc ggtcgctcat acattgaagg agatttgcag tcgttatgag ttgccctacc
      361 tgttcaaggc ctcctttgac aaggccaatc gttcgtcgct aagatccagc cgagggcctg
      421 gcatcgaaaa gggacttgag attctgaaaa ggatcaagga agaatgcgaa gtgcccgtgg
      481 ttacagacgt gcatgaagca tctcaagtga tgcctgttgc agaagttgtt gatgtgcttc
      541 aaatccctgc ttttctgtgc cgtcagaccg atttgcttat cgctgccgcg gagactggaa
      601 gaactgtgag cgtaaagaaa gggcagtttc tcgcgccatg ggagatgaag aatgttgtgg
      661 agaaaataga aagcgtggga aatcacaata tcctgttggt ggagcgtggc accacattcg
      721 gctacaacaa tcttgttgtg gatttccgat ctattccgat gatgcggtca ttgggctatc
      781 ctgtagtttt cgactgcacc cacagccttc agatgcctgg cagcttgggt gagcgcactg
      841 gaggacagcc cgaattcatc ccaacgttgg cacgggctgc ggtggccgct ggtgccgatg
      901 gtgtgtttct cgaaacgcat ccttcgccca gggacgccct gtcggacggc gcgaattgcc
      961 tgattttgac ccagtttgaa tctttgattg agtctctggc cgccttgtcc cgtgtgcgga
     1021 agcagttctc agtatagatg tgcagcatgt gtagtttttg atggaggttg acagcagagt
     1081 atgtttgccc agaatgagcg aattcgtcct gtaaacatcg aagaggagat gaaggtctcc
     1141 tacatcagct atgcgatgtc cgttatcgtc agtcgtgccc ttccggacgt tcgcgacgga
     1201 ctgaagccag ttcatcgaag agtcattttc tccatgcatg agttgggtct tgggccctcg
     1261 aggccttacc gcaagtctgc aaaaatcgtt ggggagacta tgggtaaata tcatccgcac
     1321 ggggattccg ccatctacga ttcgcttgta cgaatggcac aggacttttc cattcgatac
     1381 cccgttgtgg atggacaggg caatttcgga tcagttgacg gtgaccctcc tgcagcgatg
     1441 cggtacaccg aggctcgttt gagtcgagtc ggggaagcga tgctcactga tattgacaag
     1501 gaaacggttg acttcattcc gaactttgac gaatccgaga tggagcctac tgttttgccg
     1561 agtgtcatcc cgaacttgct ggttaacggc tctgcgggca tcgcggtggg aatggcaacg
     1621 aacattcccc cccataatct cgttgaggtg gtggacgcag ttctggctca gattgacaat
     1681 ccagatatca caactcgcga gttgatggct ctcctgccgg ggcccgattt tccgacgggg
     1741 gcgacgatct gcggacgtca gggaatactg gatgcctatg agacgggacg aggtcgtgtc
     1801 gtccagcgtg cgactgctgt cttggagcag agcaaggcag gcaagcaaaa tcttatcgtg
     1861 accgagatcc cgtatatggt caacaaggcc aatctgattc gtgagatggc tgacctgatc
     1921 cgggacaaga ggattgaggg aatcgctgat ctcagggacg aatccgatcg cgacgggatg
     1981 cgcatcgtga tcgagctgaa gcgcggtgag aactatgagg ttgtgctcaa ccagttgtac
     2041 aagcacacca agatgcagaa cacttttggc gtcatcctgc tcgctttggt ggacggacgt
     2101 ccggtgtatc tctcgctcaa ggagatcatt gaccagttca tcaagcaccg acgggaagta
     2161 atcactcgtc gcacccggtt cctgttggca aaagccgaag ccagagcgca catcttggaa
     2221 ggccttcgaa ttgccttgga caacatagac gccgtagtcg ccctgatcag aaagtcagcg
     2281 gatgcggagc aggcgaagac gggcttgatg aacaagttca aacttagcga caagcaggct
     2341 caggcgatcc tcgatatgag gctgcagcgc ttgactggct tagagcgtga gaaactcgaa
     2401 gaggagtatc gtgcgctgct gaaggacatc gagtactaca agaacgtgct ctccaatgca
     2461 gcacttctca ccgagatcat gaaaaccgaa cttagggaga tcaaagagcg attcggtgat
     2521 gaccgtcgga cgaagatcgt cgaggatgaa accgatctgg acatcgagga tcttattgca
     2581 gaagagaata tggtggtgac ggtttcgcat caaggctaca tcaagcgaat ctccacggac
     2641 acctaccggc aacagaaacg aggtggtcga gggatcatcg ctgttgagac gaaggagacc
     2701 gactttgtgg agcagatgtt cattgcttcc acgcaccatt acgtcctctt cttcaccaat
     2761 cagggacgag tgtactggca aaaggtctat gcgcttccgc aggggggacg cgcaacccgg
     2821 ggcaaagcga tcgtgaatct gcttgaactt c
//
LOCUS       DAOQUC010000391         2849 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_41460_length_2849_cov_3.198282, whole genome shotgun sequence.
ACCESSION   DAOQUC010000391 DAOQUC010000000
VERSION     DAOQUC010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2849
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41460_length_2849_cov_3.198282"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1008)
                     /locus_tag="PKH07_07760"
     CDS             complement(<1..1008)
                     /locus_tag="PKH07_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019490178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNT34877.1"
                     /translation="MSNQVAIQIEGLGKRYRLGQTVDLRRTFRETLVSLPRFVGHKAK
                     TALKTALQSDGNGVADPETPLGTFWALRDVNLQIQRGEATGIIGRNGAGKSTLLKILS
                     RITAPTKGRAEIHGRLSSLLEVGTGFHSELTGRENIYLNGAILGMRKAEIDRKFDEIV
                     AFAEIDRFLDTPVKRYSSGMYVRLAFAVAAHLEPEILVIDEVLAVGDIQFQKKCLGRM
                     GEVTQEGRTVLFVSHNMEAVRNLCQKGILLDKGKVVARGSMADVIHEYTQTGAPHESC
                     FRLPPPVEKDFPGHATELIVEDQNGTRTNSVPVGKPWQVRVRFRVLRRIEHFIMALGL
                     RT"
     gene            complement(1005..1976)
                     /locus_tag="PKH07_07765"
     CDS             complement(1005..1976)
                     /locus_tag="PKH07_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009282091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNT34878.1"
                     /translation="MKRAVVCGAGGFIGGHLVKRLKREGFWVRGVDIKTHEFCKSSAD
                     EFLLLDLRIPGNCASALDLGQEQPDEVYQLAADMGGMGFIHSAECEIMHNNVLINIHM
                     TDAAARGGVPRYFYSSSVCIYRDMKFGEPEMVEEQAYPAMPDNEYGWEKLYSERVAYA
                     FARKYGMKVRIARFQNCYGPEGTWTGGREKAPAAICRKVAEAQDGGTIDVWGDGTAVR
                     SYIYVDDLVDGIYRLMHSDLESPANIGLPQYVSVTDLVNTVADVAGKRIGIRYVDGPV
                     GVQSRNFSNEKIYSTGWKPKFMLKDGISRTYPWIEKQVQKSREQSAE"
     gene            complement(2000..>2849)
                     /locus_tag="PKH07_07770"
     CDS             complement(2000..>2849)
                     /locus_tag="PKH07_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNT34879.1"
                     /translation="PRGLVAINAREIWAYRELFAFLVWRDVKVKYKQTLLGAAWAVLV
                     PFFQMIVFTVIFGGLARIPTNGLPNPVYYMANLVIWTYFATSLSMSSQSMVGNERLLT
                     KVYFPRLIVPLAPCLASLVDFLIAFAILIGILFCYGIVPSPAIFLLPVMVLMAFGTAA
                     GAGLFLSALNVKYRDVRYVIPFLIQTWMYCSVIIPFSKFGEHFGRWRYLYGLNPMGTV
                     VEGFRWCLMHHRMTVESRVGDPVVQMAPEAPWMLFLMSVPVVLVMLCIGIAYFKRMES
                     QFADIV"
BASE COUNT          698 a          832 c          680 g          639 t
ORIGIN      
        1 cgtgcgtagg ccgagtgcca tgataaaatg ttcgatgcgt cggagcactc gaaagcgaac
       61 gcgaacctgc cagggcttgc ccaccggaac cgagttggtg cgggtgccgt tctgatcctc
      121 aacaatgagt tcggtagcgt gaccagggaa atccttttcg acaggtggtg gcagacgaaa
      181 acaggattca tgaggagcgc cggtttgggt gtactcatgg atgacatcag ccatgcttcc
      241 tctcgcaacc accttccctt tgtcaaggag aattcctttc tgacagagat tgcgaaccgc
      301 ttccatgttg tgactcacaa acaggactgt ccttccttcc tgcgtgactt ctcccatcct
      361 gcccaggcat ttcttctgga actggatatc cccaacggca agcacttcgt cgatcaccag
      421 aatctccggt tccagatgcg ccgccacagc gaaagccagg cgaacgtaca ttccgcttga
      481 ataacgcttc accggtgtgt ccaggaagcg gtcaatttcg gcaaaggcca caatctcatc
      541 gaattttcga tcaatctctg ctttccgcat gcccaggatc gccccattga gatagatgtt
      601 ctcacgacct gtcagctcac tgtgaaatcc agtccccact tccagcaatg aagacaggcg
      661 gccatggatt tccgcacgcc cttttgtagg agcagtgatt cgagagagta tcttgagcag
      721 ggtggatttg ccagccccat tacggccaat gattccagtt gcctcacccc gctggatctg
      781 aagattcacg tcccgcaacg cccaaaaggt tccaaggggt gtttccggat ccgccacacc
      841 gtttccatcg ctctgaagcg ctgttttcag agccgtcttc gctttgtgac cgacaaagcg
      901 tggcagactg acaagggtct cgcgaaaagt tcttctgagg tccacagtct gtcccaaccg
      961 atagcgcttg ccaagacctt caatctgaat ggcgacttga ttgctcattc tgccgattgc
     1021 tcccgagatt tctgaacttg cttctcaatc cacgggtagg ttcgagagat tccgtctttc
     1081 agcatgaact tcggtttcca tcccgtcgag tagatcttct cgttgctgaa gttcctcgac
     1141 tgcactccca cagggccatc tacataccga attccgattc gcttgccagc aacgtccgca
     1201 acggtattca ccaaatccgt cacagaaacg tattgaggca agccaatgtt ggccggagac
     1261 tccagatcgg aatgcatcaa tcggtagatc ccatcaacca gatcatccac atagatgtat
     1321 gaccggactg ctgttccgtc accccagaca tcgatcgttc cgccgtcttg tgcctcagcc
     1381 accttgcggc aaatggctgc cggcgccttc tctcggcctc cagtccaggt tccttcgggt
     1441 ccgtagcagt tctggaaacg cgcgattcga accttcatgc catattttct cgcaaaagcg
     1501 tatgcgactc gttcggaata gagtttctcc cagccgtatt cattgtcagg cattgccgga
     1561 taggcctgct cttccaccat ctccggctct ccaaatttca tgtcgcgata gatgcagaca
     1621 gaggacgaat agaaataacg agggacccct ccccgcgctg ccgcatcagt catgtgaatg
     1681 ttgatgagca cgttgttatg catgatctcg cattctgccg agtgaatgaa ccccatgcct
     1741 cccatgtcag cagccaactg gtagacttcg tctggttgct cctgaccgag atccagagcg
     1801 gacgcgcagt tgcctgggat gcgcagatcc aggaggagaa actcatctgc ggaagatttg
     1861 cagaattcat gggtcttgat atcaacacct cgaacccaga atccctctcg cttaagcctc
     1921 ttgacaaggt gcccgccaat gaatcctccg gctccacaaa cgaccgctct tttcatgtcc
     1981 gtagatctcc tcaactggtc tacactatat ctgcgaattg cgattccatc ctcttgaagt
     2041 aggcaatgcc aatgcaaagc atgacgagca ccacaggaac gctcatcaga aaaagcatcc
     2101 aaggagcttc aggtgccatc tgcaccaccg gatcgcccac cctactctcc actgtcatcc
     2161 gatggtgcat cagacaccac cggaagcctt ccactactgt ccccatcgga ttcagaccgt
     2221 agagataacg ccatctgccg aaatgctctc caaacttgct gaacgggatg atcaccgagc
     2281 aatacatcca agtctgaatc agaaaaggga tgacgtatcg gacgtccctg tatttgacat
     2341 tcaacgcgga gaggaaaagt cccgcacccg cagcagttcc gaatgccatg aggaccatga
     2401 cgggcagaag gaagatggct ggcgaaggca caattccgta gcagaacaga atcccaatca
     2461 gtatggcaaa ggctatcagg aaatcaacca aactcgccag gcaaggcgcg agaggaacga
     2521 tcaaacgcgg gaagtagacc ttggttagca aacgttcgtt gccaaccatg ctctggctgg
     2581 acatgctcaa tgatgtggcg aagtacgtcc agatgaccag atttgccata tagtagaccg
     2641 gattcggcaa cccatttgtc gggatgcgag ccaagccacc gaagataacc gtgaagacta
     2701 tcatctgaaa gaagggcact aggactgccc aagcggcacc caacagcgtt tgcttgtact
     2761 tgaccttaac atcccgccat accaagaagg caaagagctc ccgatatgcc catatctcac
     2821 gtgcattgat tgcaaccaga ccccgaggt
//
LOCUS       DAOQUC010000392         2849 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_41462_length_2849_cov_3.132785, whole genome shotgun sequence.
ACCESSION   DAOQUC010000392 DAOQUC010000000
VERSION     DAOQUC010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2849
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41462_length_2849_cov_3.132785"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..318)
                     /locus_tag="PKH07_07775"
     CDS             complement(<1..318)
                     /locus_tag="PKH07_07775"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNT34880.1"
                     /translation="MPVIRTSKAAMGEGNRPDWCQVTSAGIFWVPVKEGRFDCHYHDC
                     HEYWLVFQGKAKVMSEGEESYVQRGDIVCTKAGDEHDVLEVYEDLVAFWFEDATPQGG
                     RLGH"
     gene            complement(380..1393)
                     /locus_tag="PKH07_07780"
     CDS             complement(380..1393)
                     /locus_tag="PKH07_07780"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HNT34881.1"
                     /translation="MMFIHSNLINVCKLKNGLRRYMLDRFFSEQQMEPALFEIFLPQW
                     GSKTLALILHLATGQRLFVRLYRPWQRPEAEKNKAITQLLMDNGVSVPRLLGYADMPK
                     RYYSIISVEEYVESTPVVPEHYTPELAARLATQLAKLHSIRAPRWGQPPEPSGRSGFG
                     SYIIRHVLNRVSRIQQHRVLGAIPNRKELIAQLRQWKNELNLIPQYNLTHDKIPGRNV
                     ILSPDGRLFFIDFPTAYYSFFAKDLVSFHHRFCSDDEEKIEVFNAAYFASMLPEDKTR
                     FERLHPFYDAWFHLSRCAENANAAQKCFRAGEGYGREYQKKSQEHWETASCILKEHPP
                     RQG"
     gene            complement(1559..>2849)
                     /locus_tag="PKH07_07785"
     CDS             complement(1559..>2849)
                     /locus_tag="PKH07_07785"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HNT34882.1"
                     /translation="LADCTRDEIQAVAAHELAHILRGDALYVTFVCSLVNVFERLREF
                     FEPSTATSGDTQHRDAAGGSLIYVAAWLSSTMMLLFSALLSRQREFMADAAAVELCRD
                     PMALSRAIYKANTRYSQVGGTSDVYSPIFIVDPRSVGLEDDSGFFGRLFGSHPAAMKR
                     IERLAAMAGKRATDVLGEIRDIQKERSRNKTVIKHFVSSHPEQDAGVQSEDPTWLACD
                     GKGQWHGPYRVEELVFLPFFTTRVRVRNLIENQAGLASDFKPIVELLNRAGTHARHPD
                     SNQCPRCKVALSQSLYEGTTIHACARCGGKYLNTSALDKILVRRDVVFDETLCQKARE
                     FRDTVLLNPLKRKPGREAASPMLYTSPLTAKQVPKSPQSETLLCPTCSAMMIEHPFSY
                     QYFVPVFKCLLCRTFWFDPDNLEILQILVESNRETEF"
BASE COUNT          690 a          762 c          738 g          659 t
ORIGIN      
        1 atgtccgagc cgtccccctt gcggcgtggc atcttcgaac cagaaagcga cgagatcctc
       61 atagacttcg agaacatcgt gctcatcacc cgctttggtg caaacaatgt ccccacgctg
      121 cacgtaagac tcctcacctt ccgacatgac cttcgctttc ccttggaaaa ccaaccaata
      181 ctcatgacag tcatggtaat gacaatcgaa acgaccttcc ttgacaggaa cccagaagat
      241 tcccgcgctg gtcacttgac accagtcagg gcgattcccc tcgcccatcg ctgctttcga
      301 agtccgaatc acaggcatgt tcttttctcc tctttgatca ttgtctgagt agcaatgcta
      361 tcatgaaacc gttttcagat catccttgac gaggcggatg ctccttcaga atgcaggagg
      421 ccgtttccca atgctcttgg gatttcttct ggtactcgcg tccgtaaccc tctcctgcac
      481 ggaaacactt ttgtgccgcg ttggcgttct ccgcgcaacg ggaaagatga aaccaggcat
      541 cgtagaatgg atgcagtcgc tcaaagcgcg ttttatcttc aggcaacatt gacgcaaagt
      601 aggccgcatt gaatacttcg atcttctctt cgtcgtcaga acaaaagcgg tgatgaaaag
      661 agacaagatc cttggcgaag aaagagtagt acgctgtggg gaaatcaatg aagaacaacc
      721 gaccgtcggg agacaaaatc acatttcttc ctggaatctt gtcatgagtc agattgtatt
      781 gtggaatcag gttcagctca ttcttccatt gccgcaactg agcgatcagc tccttcctgt
      841 ttggtatggc acccagaact cggtgctgct gaatgcgcga aacccgattg agaacgtgac
      901 ggatgatgta tgagccaaag ccggatcttc ccgagggctc aggcggctgc ccccacctcg
      961 gagcgcgaat cgaatgcagt ttcgccaact gagttgccag gcgcgcggca agttcgggag
     1021 tgtagtgctc gggaacgacc ggtgtgctct cgacatactc ttcaaccgag atgatgctgt
     1081 agtaacgctt gggcatatcc gcataaccaa gcaatcttgg aaccgaaaca ccgttgtcca
     1141 taagcagttg tgtgatcgcc ttgttcttct ctgcttcggg tcgctgccag ggtcggtaaa
     1201 gccgaacgaa taggcgctgt cccgtcgcaa ggtggagaat caaggcgagc gtcttggaac
     1261 cccactgggg aaggaatatc tcgaaaagag caggttccat ttgctgttcg gaaaagaatc
     1321 ggtcgagcat gtaacggcgc agcccgttct tcagcttgca cacattgata aggttgctgt
     1381 gtatgaacat catgactctt ccaggttgcc tctcgacaac ggagacttga aacacgtgat
     1441 actagtgagg cgacgctttg agtgtcaaga aaatgtgtca acctgccagg tgtcaccgag
     1501 cggacgtgga caccgtgagt caatgcctct ttcaagatcg cgcagggaac acaccttttc
     1561 agaactctgt ctctcggttg gattcaacaa gaatctgcaa gatttccagg ttgtccggat
     1621 caaaccaaaa ggtgcggcac aggagacatt tgaacacggg cacaaagtac tgataactga
     1681 acggatgctc gatcatcatc gcactgcatg tggggcacag aagcgtctcg gactgcggcg
     1741 atttgggaac ctgtttggct gtgagcggac tcgtgtaaag cattggcgag gccgcctccc
     1801 gtccaggttt ccttttgaga gggttaagca gcacagtatc tcgaaactct cttgctttct
     1861 ggcatagagt ttcatcaaac accacatctc gtcgaaccag tatcttgtcc agggcagagg
     1921 tattgagata ctttccgcca caacgagcgc acgcgtgaat tgttgtccct tcgtacaaac
     1981 tctgactgag agcgaccttg cagcgtggac attggttgct gtccggatgt ctcgcatgcg
     2041 ttccagcccg attcagaagt tcgacaatgg gcttgaaatc ggaagcaagt cctgcttggt
     2101 tttcgatcag atttctgaca cgaacacgcg ttgtaaagaa gggaagaaaa acaagttcct
     2161 cgactcgata ggggccatgc cactgtcctt tcccatcaca ggcaagccag gttggatctt
     2221 cgctctggac acctgcgtct tgctcaggat ggcttgacac gaagtgcttg atcaccgtct
     2281 tgttccgcga gcgttctttc tggatgtcgc gaatctctcc caggacatcg gtcgcgcgct
     2341 tgcccgccat cgcagccagc cgttcgatac gcttcatggc agcggggtgc gaaccgaaga
     2401 gtcgtccaaa gaatccgctg tcatcttcga gaccgacgga acgaggatcg acgatgaaaa
     2461 tgggactgta gacatcgctg gttcctccca cttgagagta gcgtgtattg gctttgtaga
     2521 tagcccgcga aagtgccatc ggatcacgac acagttccac cgccgctgca tctgccatga
     2581 attcacgttg gcgactcaac agggcactga acaagagcat catggtggac gaaagccacg
     2641 cagccacata gatcaatgaa ccgcctgccg catctcgatg ctgcgtgtca cccgaggtcg
     2701 ctgtggatgg ctcaaagaac tcgcgcaggc gctcaaaaac gtttaccagg gaacagacaa
     2761 atgtaacgta cagggcatct ccccgaagaa tatgagccag ttcgtgcgca gcgacagcct
     2821 gaatctcatc tctcgtgcag tctgcaagg
//
LOCUS       DAOQUC010000393         2849 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_41463_length_2849_cov_3.115963, whole genome shotgun sequence.
ACCESSION   DAOQUC010000393 DAOQUC010000000
VERSION     DAOQUC010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2849
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41463_length_2849_cov_3.115963"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..573)
                     /locus_tag="PKH07_07790"
     CDS             complement(<1..573)
                     /locus_tag="PKH07_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HNT34883.1"
                     /translation="MLKDCRISETFSPDKAVVVFPGDCRDLLSQVPDDSIQLIVTSPP
                     YNLGKEYERKLQIDAYLSQQRNVIRECVRVLAPQGSICWQVGNYVDNGAIIPLDTVLF
                     PIFSELGLKMRNRIIWHFEHGLHCKRRFSGRYETIIWFTKTKDYTFNLDPVRVPQKYP
                     GKKYFKGPKAGEYSCNPLGKNPGDLWVIPNV"
     gene            733..1764
                     /gene="hisC"
                     /locus_tag="PKH07_07795"
     CDS             733..1764
                     /gene="hisC"
                     /locus_tag="PKH07_07795"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HNT34884.1"
                     /translation="MDWTRLDLKDVEGYVPGEQPQMARLVKLNTNENPYPPSPLVKET
                     FDKFSVESLRLYPDPMMTELRKTASDVYGVAADWIIGGNGSDEILSMLLRCAVSPKEK
                     VWTVYPTYSLYPILVRIAGGEPASMDLKDDGALPAGLADVPGRLLLLANPNPPLGTVY
                     SLQEIEQLCLAQNKIIAVDEAYADFAGVSAISLLEKYENLVVIRTFSKSYSLAGVRLG
                     LAFCRGEIRDAMLKVKDSYNVNRLTQALGIAALKDQACLRQNVAKIVKTRKRLTESLE
                     ELGFTVFPSGGNFVFAKPPGRNAREVFEKLRSQGVLVRYFPVRRLDDGLRITVGTEPE
                     IERLLEILQ"
     gene            1815..2420
                     /gene="hisB"
                     /locus_tag="PKH07_07800"
     CDS             1815..2420
                     /gene="hisB"
                     /locus_tag="PKH07_07800"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HNT34885.1"
                     /translation="MPKKTTKERWALVARKTKETDIEVSMTLDGTGTQDISTGVPFLD
                     HMLTLFARHGRFDLRIQAQGDTQIDAHHTVEDVGICMGKALLEALGDRGGIRRYGHVA
                     LPMEEALVLAAIDISGREGFFSALGEMKPKVGEFDVELVPEFFRAFTSNGEFTLHLRV
                     LAGTNTHHVIEALFKACAVALRNAVEVVDPSLKIPSTKGIL"
     gene            2427..2648
                     /locus_tag="PKH07_07805"
     CDS             2427..2648
                     /locus_tag="PKH07_07805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34886.1"
                     /translation="MAESSRPIKIVRVIARLNIGGPAVHTILVTQALQGTKYENILVA
                     GVESKDEGSMDFLAAQHEVQPLILRGLGR"
     gene            2658..>2849
                     /locus_tag="PKH07_07810"
     CDS             2658..>2849
                     /locus_tag="PKH07_07810"
                     /inference="COORDINATES: protein motif:HMM:NF024968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNT34887.1"
                     /translation="MDDVKTLVRLYRLFRKERPDIVETHTAKAGAVGRVAAWLARVPV
                     RIHFFHGHVLYGYFGKLLTW"
BASE COUNT          652 a          704 c          789 g          704 t
ORIGIN      
        1 gacattcgga atcacccata gatccccagg attctttccg agaggattgc atgagtattc
       61 tcccgccttc ggccctttga agtacttctt gcccggatac ttttgaggca cgcgaactgg
      121 atccagattg aaggtgtaat ccttggtctt ggtaaaccag attatcgttt cgtaacgacc
      181 agaaaaccgc cgcttgcagt gtagcccatg ctcaaagtgc cagatgatcc tgttgcgcat
      241 cttcaaaccc aattcgctga aaatggggaa taaaactgta tccagtggaa taatcgcgcc
      301 attatctacg taattcccga cctgccaaca gatgctccct tggggagcaa gcacacgtac
      361 gcactctctg atgacattcc gctgttggct gagataggcg tcgatctgta gtttcctctc
      421 atactccttc ccaagattgt agggcggaga ggtcacaatg agttgaatcg aatcatcagg
      481 cacctgggat aacagatcac gacaatctcc ggggaagacc acaacagcct tgtccggtga
      541 aaaagtttca gatattctgc agtccttgag catgtctgca tcatacgcca tgcctgacat
      601 tggggtcaag gaattcacca tccctaagag agtcattgca gtcgtgagac tgatttttct
      661 ttctattccc attgaactat agtagctttt aagtttgttg ccaagagaat cagacatcga
      721 ggaggcacgt ctttggactg gacgcgtctg gatttgaagg acgttgaggg atatgttccc
      781 ggggagcagc cgcagatggc gcggctggtc aagctcaata ccaacgagaa cccatatcct
      841 ccttcgccgc tcgtgaagga gacttttgac aagttttctg ttgaatcgtt gcgcctgtat
      901 cccgatccga tgatgaccga gttgcgcaag accgcttctg acgtctatgg cgttgctgca
      961 gactggatca tcggcggaaa tggctcggat gaaatcctga gcatgctgtt gcgctgtgct
     1021 gtttcgccaa aggagaaggt ctggactgtc tatccgacgt actccctcta tcctattctg
     1081 gttcggatcg cggggggaga gcctgcctcg atggacttga aggatgacgg agcactgcct
     1141 gcaggtcttg ccgacgttcc aggccgactg ctcctgcttg cgaatccgaa tccacccttg
     1201 ggaacggtct acagcctgca ggagatcgag cagttgtgtc ttgctcagaa caagatcatc
     1261 gcggtggatg aggcctacgc ggatttcgcc ggcgtcagcg ccatttcact gcttgagaaa
     1321 tacgagaatc tggtggtcat tcgaacgttc tcgaaatctt attctctcgc gggagtgcgg
     1381 ctgggacttg ccttttgccg tggagagatt cgggatgcaa tgctcaaggt caaggattcc
     1441 tacaacgtca atcgcctcac gcaggccttg ggaatcgctg ctctcaagga tcaggcttgt
     1501 ctaagacaga atgttgcgaa gattgtcaag acaagaaaga ggctcacgga gtccctcgaa
     1561 gaattgggat tcactgtctt tccttcggga ggaaacttcg tctttgccaa accgccgggg
     1621 agaaatgctc gtgaggtatt cgaaaaactg cgctctcagg gcgttctggt tcggtacttt
     1681 cccgttcgtc gcctggatga cggattgagg atcacggttg gcacggagcc ggagatagag
     1741 aggctgcttg aaatccttca gtgagatatt tttggaagtc ttgaacagag aagatggagg
     1801 cgaaggtgaa gaagatgccc aagaaaacaa caaaggaacg ctgggcgctt gtggctcgaa
     1861 agaccaagga aacggacatt gaggtcagca tgacactcga tgggactggg actcaggata
     1921 tttccacggg tgttcctttt ctggatcaca tgttgacgct ttttgcacgg catggtcggt
     1981 ttgatcttcg cattcaggcg cagggagaca cccagattga cgcgcatcac acagttgaag
     2041 atgtgggcat ctgcatgggt aaggccttgt tggaggcact tggcgaccgt ggtggcatcc
     2101 gccgctatgg ccacgtcgca ttgccgatgg aagaggcgct tgtcctggcg gcgatagaca
     2161 tttccggtcg tgaagggttt ttctccgcct tgggtgagat gaaaccgaaa gtcggagagt
     2221 ttgacgtcga attagtgccc gagttcttcc gagcatttac atccaacggt gagttcacgc
     2281 tgcatctgcg cgtgctggcg ggaaccaaca cccaccacgt catcgaagcg cttttcaaag
     2341 cctgtgccgt cgcgctccga aatgctgtgg aagtggtgga tccttcactc aagattcctt
     2401 cgaccaaagg aattctctaa aaactcatgg cggagtcttc cagaccaatc aagatcgttc
     2461 gcgtcattgc acgcttgaac attggggggc ctgccgtgca tacgattctt gtcactcagg
     2521 cgctccaagg caccaagtat gaaaacatcc ttgtggcagg tgtcgagagc aaagacgaag
     2581 gcagtatgga tttccttgcc gctcagcacg aagtgcagcc cttgattctg agaggcttgg
     2641 ggcgctagat ctccattctg gatgatgtca aaacgctcgt tcgactctac cgtctctttc
     2701 ggaaagaacg tccggacatt gtggaaaccc atacggcaaa agcgggggcg gtgggtcgcg
     2761 ttgcggcttg gcttgcacgg gtgccggttc gcattcattt tttccacggt catgtcttat
     2821 acggctattt cgggaagctg ctcacctgg
//
LOCUS       DAOQUC010000394         2848 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_41484_length_2848_cov_3.432152, whole genome shotgun sequence.
ACCESSION   DAOQUC010000394 DAOQUC010000000
VERSION     DAOQUC010000394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2848)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2848)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2848
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41484_length_2848_cov_3.432152"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            130..2493
                     /gene="lon"
                     /locus_tag="PKH07_07815"
     CDS             130..2493
                     /gene="lon"
                     /locus_tag="PKH07_07815"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:NF008053.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HNT34888.1"
                     /translation="MRAKKITKKTRAGGEVKSDLEQLPLLPLRDVVVFPNMIQPLLVG
                     RKRSVAAVEKALEKDKRLFIVTQQQPETEDPGLKDLYPIGTLSVVHQVVRLPDGSMKV
                     LMEGVTRCQLVDVFPVPEMILCWVKPVEEPTSSGSEVEALIRLVSSLFENYVKLNPRV
                     PQETLMVLYNVSSDPHRLADAVGSNLFIDVQEKQGILSTLDPVERLRKLCEILNSENE
                     ILELEQNIKSQVRKQMEKTQREFYLNEQIKVIQRELGREGDMAEEVLELEEKIAAAKM
                     SPEADEKARKELTRLQHMPMMSPEAAVVRTYLDWLVTLPWKKSSKEQLDIEQAEKILD
                     EDHYGLKKPKERILEYLAVRKLSKHLRGPILCFVGPPGVGKTSLARSIARALNRKFVR
                     VALGGVRDEAEIRGHRRTYIGALPGRIIQSLKRVKTNNPVFLLDEVDKMSTDFRGDPS
                     SALLEVLDPELNNTFSDHYLEVEFDLSDVLFIATANTKYPIPPALRDRMEIIELPSYT
                     EQEKLAIAEQFLVRKQIRANGLSRKRVTFTTDALKTLVRGYTKESGVRNLEREIANVL
                     RKVAKAIASNKAKGGLVIDGTAVEKYLGVPKFRQTEIERGGGVGLATGLAWTEFGGEV
                     LTTEVSIMEGRGELVLTGKLGDVMQESAKAALTYTRMQTRRFGLARDFHQRFDIHVHI
                     PEGAIPKDGPSAGITIATAIISALTGMSVRQDLAMTGEITLRGRVLPVGGIKEKVLAA
                     HRAEIHTVLLPDENRKDLEDIPNAVLQELEIHFVKNMDEVIERALVE"
     gene            2529..>2848
                     /gene="yihA"
                     /locus_tag="PKH07_07820"
     CDS             2529..>2848
                     /gene="yihA"
                     /locus_tag="PKH07_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012031979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HNT34889.1"
                     /translation="MKRIDFSQAQLLACAASPRDFPPPTLPEVAFLGRSNVGKSSLIN
                     RLTRRKNLAKTSSTPGKTRMAIFFDVQGILRLVDLPGYGYAAVNVSMRQQWQTLMDAY
                     FARS"
BASE COUNT          682 a          670 c          834 g          662 t
ORIGIN      
        1 attgccttgt cgggaatcaa aggttgatcg ttttgcacga tctgtcctat aggagtttcc
       61 gaggggttgt tttgcgggac aacgcacttt gtgctatata cgagccgaca ttttagaaag
      121 ggctttttga tgagggcaaa aaagataacc aagaagactc gagccggtgg tgaagtcaag
      181 agcgaccttg agcagttgcc tcttcttcca ctccgggatg tcgtcgtatt tcccaacatg
      241 atccagcctc tgttggtagg tcgcaagaga tcagttgcgg ctgtggagaa ggcactggag
      301 aaggacaagc gtctctttat cgtgacgcaa cagcagcctg agacggaaga tcccggcctg
      361 aaggatctct atccgattgg gactctcagc gtggtgcatc aggtcgtgcg tctccccgac
      421 ggttcgatga aggttttgat ggaaggcgtg acgcgctgtc aattggtgga tgtttttccg
      481 gtgcccgaga tgatcctctg ttgggtcaaa ccagttgaag aaccgacatc ttcgggttct
      541 gaagtggaag ccctgattcg gcttgtcagc agcctttttg aaaactatgt caaactgaat
      601 ccaagggtgc ctcaggagac gctcatggtg ctctataacg tctcctctga tccccatcgt
      661 ctggcggatg ccgttggttc caacttgttc attgacgttc aggaaaagca ggggattctc
      721 tcgactctgg atccagttga gcggcttcgg aaactctgtg aaatactgaa ctcggagaat
      781 gagattctgg aacttgagca gaacatcaag tctcaggttc gaaagcaaat ggagaagacg
      841 cagcgcgaat tctacctcaa tgagcagatt aaggtcatcc agcgcgaact ggggcgcgaa
      901 ggggacatgg cagaggaggt tctggaactc gaagagaaga tcgctgcagc caaaatgtct
      961 cccgaagcgg acgaaaaggc tcgcaaggaa ctgaccagac ttcagcacat gccgatgatg
     1021 tctcctgaag cggccgttgt tcgaacttat cttgactggc tggttactct gccttggaag
     1081 aagagttcca aagagcaact cgacattgag caggcggaga agattcttga tgaggatcac
     1141 tacggactga aaaaacccaa ggaaaggatt ttggaatacc ttgcggttcg gaaattgtcg
     1201 aaacatcttc gagggccgat tctctgtttc gtcgggccgc ctggcgtggg gaagacttcc
     1261 ttggcgcgtt ccatagcgcg tgccctgaat cgcaagtttg ttcgcgttgc ccttggtggg
     1321 gttcgcgatg aagcggaaat acggggtcat cgaagaactt atattggcgc tcttcccgga
     1381 cgcatcatcc aatcgcttaa acgtgtaaag accaataatc cggttttcct tctggatgaa
     1441 gtggacaaga tgagtacaga ctttcgggga gatccttcat ctgcattgtt ggaagttctg
     1501 gacccggaac tgaacaacac attcagtgac cactatcttg aggtcgagtt cgatctctca
     1561 gatgtgcttt ttatcgccac ggccaacaca aaatacccca ttccgcccgc gcttcgcgat
     1621 cgaatggaga ttatcgaact gccgtcgtac accgagcagg agaaacttgc catcgcggag
     1681 cagtttcttg tccggaagca gattcgggcg aacggtctga gccgcaagcg agttaccttc
     1741 acgaccgatg cactgaagac actcgttcgg ggctatacca aggaatctgg agtcaggaac
     1801 cttgagcgtg agattgccaa tgtccttcgg aaagttgcca aggcgattgc cagcaataaa
     1861 gccaaaggag gcctggtcat tgatgggacg gcggtcgaga agtacctggg tgtaccgaaa
     1921 ttccgtcaga cagaaattga acgtggtggt ggcgtaggtc ttgcaacggg actcgcgtgg
     1981 actgaattcg gtggagaagt gcttacgacg gaagtctcga ttatggaggg gcgtggggaa
     2041 ctggttctga ctggaaaact cggcgacgtc atgcaggaat cagccaaggc agccttgacc
     2101 tatacgcgga tgcagacgag gcgcttcgga ttggcgcggg attttcacca acggtttgac
     2161 atccatgttc acattcccga gggagccatt cccaaggacg ggccatctgc tggaatcacc
     2221 atcgcgaccg cgatcatctc ggcgctgact gggatgtcgg tacgtcagga tctggcaatg
     2281 acgggcgaga ttacgcttcg tggccgtgtc ctgcctgtcg gggggatcaa ggagaaggtt
     2341 cttgcggcac atcgagcgga gatacacacc gtgctgctac cggatgagaa caggaaagat
     2401 ctggaagaca ttccgaacgc agtgcttcag gaacttgaaa tccacttcgt caagaatatg
     2461 gatgaagtga ttgaacgtgc cttggtggag tgaacggggc gaaataacgc tggactggag
     2521 tccgaggttt gaagagaatt gacttctcgc aggcgcaact actggcttgt gctgcaagcc
     2581 caagagactt tccgccgccg acgcttccgg aagtggcctt tctcgggcgt tccaatgttg
     2641 gcaaatcgtc gttgattaac cggctgacgc gacgaaagaa ccttgctaaa acgtccagta
     2701 ctcccgggaa gactcggatg gcgatctttt tcgatgtgca gggcattctt cgtttggtgg
     2761 atcttcccgg ctatggctat gcggcggtca acgtttcgat gaggcagcag tggcagacac
     2821 tgatggacgc ctactttgca cggagtga
//
LOCUS       DAOQUC010000395         2844 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_41583_length_2844_cov_3.683041, whole genome shotgun sequence.
ACCESSION   DAOQUC010000395 DAOQUC010000000
VERSION     DAOQUC010000395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2844)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2844)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2844
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41583_length_2844_cov_3.683041"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="PKH07_07825"
     CDS             complement(<1..252)
                     /locus_tag="PKH07_07825"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNT34890.1"
                     /translation="MKRTGKHGFTLLEVIIALGLLGLGLASILQAFPGAVRSSVRVQY
                     NSRASLLAQQIIETLRADSKNLERSASNLLPIFHDFDQNE"
     gene            complement(627..1409)
                     /locus_tag="PKH07_07830"
     CDS             complement(627..1409)
                     /locus_tag="PKH07_07830"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNT34891.1"
                     /translation="MKNRQSGFSLVEMLVVLAIIVVMGGVVSFSYWNVLHSRRVAGGA
                     EAVWSALSSSRQAAITMRESRALELDLLEDSFQILREVYRYDRATGKETSEWESSGGA
                     EQILASVDVTDICFSSDDVPQDMDQWLAIVQRQAKVRFVFNRRGALSEVSYQVSKDQW
                     QTVESKNPIIHVYSLGRDGSDKVHGSQIFLAGRAYPYRDPKNTRNDVLNQADLRTKLR
                     GLPVDSRERRKCYSVKLYGATGRSIVYDYGIGYPWPSSMMYE"
     gene            complement(1572..>2844)
                     /locus_tag="PKH07_07835"
     CDS             complement(1572..>2844)
                     /locus_tag="PKH07_07835"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HNT34892.1"
                     /translation="FHVADTWWVSRFLGAEATAALGSSGFFAWAIFSIGMMASDGVNA
                     MVARFTGAGDRRMTGEVAYQGFVLAMIVSMVVMVAGFAFKQPIFGFLQTEDSVTTLAY
                     QYLDIIIWGASTVMLFMVVTAIFRAIGDTTTPLILLVFAGSMNFLLDPLLMLGLGPFP
                     RLGVQGAALATVISRLFGVVFGVYLLCRHRGGGKVDWHLPQWMPNPRMYLRILRIGIP
                     SSLSGLLFSLVYFCLLRVIAQVSPENRTVSVAALTIGFRVESFAFMWSAGFSTAAATL
                     VGQNLGSGKPDRAEQCAWRSALTLCLITGTAGVFMMIYPKQLVGLFSKDPDVIVRAAS
                     YVWVMGVIQVPQSLAGVLRGAFAGAGNTLPTLLIDAPMNFARVPLGYLMAIPLGLGLQ
                     GVWWAIGLSNVGKGLTLSAWFSRGRWKHHKV"
BASE COUNT          766 a          821 c          673 g          584 t
ORIGIN      
        1 ctcgttttga tcgaaatcgt gaaagattgg cagcagattg ctggcgctgc gttcaaggtt
       61 cttgctgtct gccctgagtg tctcgatgat ctgttgtgct agcaaagaag cacgggaatt
      121 gtactgcaca cgaacgctgg agcgaactgc cccaggaaac gcctgaagaa ttgaggcaag
      181 gcccagtcca agcaagccca gggcaatgat cacttcaagc agtgtaaacc catgttttcc
      241 agttcgtttc aagcgatgtc agccctttct ttcgcagacg gaatgtgtga acgctccaca
      301 ttcacggatc ataccacatt ttctcaaagg aatagtagca taattcagac cacgagtcac
      361 cctttatgcc aagcctgttg cacaccttcg aagagacggc aaacggagca taccagaaag
      421 atttttcgaa gaaacagtca cactcgagca tcaaattcat ccgtcctgtc agtacaggtc
      481 agcttgcaag gagcgttggt gtgcattttt gttcacacta atgcgcagtt tacttcccag
      541 aatctgcatt tagaagactc aaaacccttt gatgattcgg ttgatagaaa tggtttctta
      601 agatttgaaa tgaaactccg acaacgctac tcatacatca tggaactcgg ccatggatac
      661 ccgattccgt agtcatagac aatggatctt cctgtcgccc catagagttt gactgagtag
      721 cacttccggc gctccctgga gtccaccgga aggcctctga gttttgttcg aagatcggcc
      781 tgattcagaa cgtcgttccg cgtgttcttg ggatcccgat acggataggc tcttccggca
      841 agaaatatct gcgagccatg gactttgtcg gaaccgtcac gacccaatga gtagacgtga
      901 ataatcggat tcttactttc aacggtctgc cactgatcct tggagacttg ataggagact
      961 tcggacaagg caccacgtct gttgaataca aaacgaacct tcgcttgcct ttggacaatt
     1021 gccaaccatt gatccatgtc ttggggaacg tcatcggaag agaagcaaat atcggtcaca
     1081 tccaccgagg caagaatctg ctccgctcca ccagaagact cccattctga tgtttccttg
     1141 cccgttgcgc gatcgtaccg gtagacttcg cgaagaatct gaaatgagtc ttccagcaga
     1201 tcaagttcca gtgctcggga ctccctcatc gtgatggctg cctgcctgga cgaggacaaa
     1261 gccgaccaaa cggcctcggc tcctccggcg actctcctgc tgtgaagcac gttccaataa
     1321 gaaaatgaga ccactccacc catgacgacg atgatcgcca gcaccaccag catttccacg
     1381 aggctgaaac cgctttgacg gtttttcata tagcgcttct cctgacagga tgccgcctca
     1441 tcgtcgaagg ggctgtactg cgtccttcaa tcatctgcca acctgactat gaatcttatc
     1501 gcttgcctcg ttttctgtca acgaagccac tacactgaag caaaagcacg acgacccgct
     1561 cttggaaaac gtcacacttt gtggtgtttc caccgaccgc gggaaaacca ggcactcagc
     1621 gtaagcccct tccctacgtt cgaaagcccg atcgcccacc agactccctg aaggccaagt
     1681 cccaaaggaa tcgccatcag ataccccaac ggaacacgag cgaaattcat cggtgcgtca
     1741 atgagtagag tcggcagcgt attgcctgca ccggcaaagg caccacgaag aacaccggca
     1801 agagactgcg gaacctggat gacgcccatg acccaaacat aggaagccgc acgaacaatg
     1861 acatcaggat ctttgctgaa caatcccacc aactgcttcg ggtagatcat catgaacact
     1921 cccgctgtgc ccgtgatcag acacaaggtc aaagcggaac gccaggcaca ctgctctgcg
     1981 cggtcgggct tgccggaacc aaggttctgg ccgaccagcg tcgccgcagc agtactgaat
     2041 ccagccgacc acataaaagc aaatgactcc acgcgaaaac caatggtcag ggcggcaacg
     2101 gagactgtcc tgttctccgg tgaaacctgg gcgatcacac gcagcagaca gaagtatacc
     2161 aacgagaaca atagccccga aagactgctc gggatgccga ttcgaagaat ccgaagatac
     2221 attctcggat tcggcatcca ttgtggcaga tgccagtcaa ccttccctcc acctcgatga
     2281 cggcagagca gatagactcc gaagaccact ccaaacagac ggctgatcac ggttgccaga
     2341 gccgcaccct gcactcccag gcgaggaaag ggtcccaagc ccagcatcag aagcggatcc
     2401 aacaggaaat tcatcgaacc tgcaaaaacc aacaagatga gcggagtcgt tgtgtcccca
     2461 atggcgcgaa atatcgccgt gacaaccatg aacaacatca ctgtcgaagc gccccagatg
     2521 atgatatcga ggtattggta ggccaatgtc gtcacactgt cttccgtttg gagaaatcca
     2581 aaaatcggct gcttgaaggc aaaaccagcg accataacca ccatcgagac aatcatcgcg
     2641 agaacgaagc cttggtaagc cacttcgccc gtcatgcgtc ggtcgcccgc gccagtgaaa
     2701 cgtgccacca tcgcattcac tccatcggaa gccatcatgc caatggagaa tatcgcccag
     2761 gcaaaaaaac cgctgcttcc aagcgctgcc gtggcctcag cgcccagaaa ccgactgacc
     2821 caccaggtat ccgccacatg aaag
//
LOCUS       DAOQUC010000396         2844 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_41590_length_2844_cov_2.920760, whole genome shotgun sequence.
ACCESSION   DAOQUC010000396 DAOQUC010000000
VERSION     DAOQUC010000396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2844)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2844)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2844
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41590_length_2844_cov_2.920760"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(364..1890)
                     /locus_tag="PKH07_07840"
     CDS             complement(364..1890)
                     /locus_tag="PKH07_07840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34893.1"
                     /translation="MFMGRRCLVVTVVGFMFVLFVSGSLAETAADAGKLIGEAQRLFF
                     QGKTDEADASLKKVETMMSDAQKNNDQDSLTKLKLVDGRVKTLRKQIDTKLGKTAAPA
                     PVAVQQEQPATTQAASQQALPSTVTTRLKKITEAVEQGHSWLEQDQKSSARNSHETAQ
                     RLMDEITQSYGTQISMEHPDIVAAKQSIAGLQSKLAEGDAKVAAEKEAAAQSAVQAKM
                     TSDEWLVKLKPYAMGMGRPEYDPERYFVGSYTEEMEEMNKRMRIFGLVQAEMESYHAS
                     GPGENASDELAEIVRQLDYEMKTFQESCKTMANLNVDDAERDIDHLLTRIDEESKKVG
                     TGDLPILMDKFTFENAKAKLDRAANLLGSDDARIVTVTGKYTTLLEKDAAIRKARVAD
                     TRMIGDKYTGSDTETLKKKAQDVVLAKNAGAKVLRTTLINDDWREENVVEWTDTTKSA
                     LRHRVTRYMAGQVAAKTGDETKIYTVHLAKDRRTDGSWGELQGHIMFTDPILEENVNK
                     "
     gene            complement(2145..>2844)
                     /locus_tag="PKH07_07845"
     CDS             complement(2145..>2844)
                     /locus_tag="PKH07_07845"
                     /inference="COORDINATES: protein motif:HMM:NF024687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HNT34894.1"
                     /translation="PVKMLEDPEKMTRVFEDFHLRTLDELYVSVGFGKVLTKHVISRL
                     LPKTQLFQKKQASEATPGMIPIRDIDGYSYRRALCCNPLPGDPIVGFVTKNRGISIHA
                     KECQSLASANVSEARLLPLYWDRGESESFQVEIGVEATDRERLLSDITSVIASTGTNI
                     RGSVARSQDDGTAKLVFMMDVVDVDHVNRVINRLIPIPGVKTVIRRTKPSRQLSDAAS
                     TSPKGKRKRTHKKK"
BASE COUNT          590 a          801 c          719 g          734 t
ORIGIN      
        1 aactgccgaa gtgttctgtc cgcttcctaa cgagaagcag catcttgaag tcggtttcct
       61 gagcactccg gatgccatcg tacttgagac gatatttgag taaaagacac agaacgacga
      121 aactgcagaa acgaacgatc cggagttgat ctgcaccgta gtcaagccgt gtgcgggtag
      181 gtgtccgcat agatcttaat gctgaactat ggttctgggc gcagcggaga gttcactgtc
      241 cacagatgtc gcagatttcg cagtgcacca cgtccacttc atcaaacggc gatggatcag
      301 aagccaccac gggctgacaa gggcacagcg aactccacag aaccgactac gccagttcga
      361 agatcacttg ttcacattct cttcgaggat cggatctgtg aacatgatgt gaccctggag
      421 ttcaccccag gaaccgtccg tgcgtcgatc ctttgccaag tgaacggtgt agatttttgt
      481 ctcgtcgcca gtcttcgcgg caacctgtcc ggccatatac cttgtgacac ggtgacgcag
      541 ggcggacttg gttgtgtccg tccattcaac gacgttttcc tcacgccagt cgtcattgat
      601 aagtgtggtg cgaagaacct ttgctcctgc gttctttgcc aggacaacgt cctgtgcctt
      661 ctttttaagt gtctcggtgt cggatccggt atacttgtcg ccgatcattc tcgtgtccgc
      721 gactcgcgct ttgcggatcg ccgcatcttt ctccaacagc gtcgtgtatt tgcctgtcac
      781 cgtaacgata cgcgcgtcat ctgaaccgag cagattcgcc gctctgtcga gtttggcttt
      841 ggcattttca aaagtaaact tgtccatcag aattgggaga tcccccgtac ccactttctt
      901 ggactcctcg tcaattcgcg tcaggagatg gtctatgtct ctctccgcgt catccacgtt
      961 gagattggcc atcgtcttgc aggactcctg gaacgtcttc atttcgtagt ccagttggcg
     1021 cacgatttcc gccagttcgt ccgaggcgtt ctctccaggc ccactggcgt ggtagctctc
     1081 catctcggcc tgcacaagac cgaagatcct catgcgcttg ttcatctctt ccatttcttc
     1141 ggtatagctg ccgacgaagt accgctccgg atcatattcc ggacgcccca tccccatggc
     1201 gtaaggcttc agtttgacga gccactcatc ggacgtcatc tttgcctgga cggcggactg
     1261 tgctgctgcc tctttctctg ctgccacttt cgcatcgcct tccgccagtt tggactgcaa
     1321 gccggcgatg gactgcttgg cggcaacgat gtcggggtgt tccatggaaa tctgagttcc
     1381 atagctctga gtaatttcgt ccatgagtct ctgtgccgtt tcatgggagt ttcgagcgga
     1441 tgacttctga tcctgctcaa gccaactgtg gccttgttcc accgcctcgg taatcttctt
     1501 cagtcgtgtg gtgacggtag aaggcaacgc ctgctgtgac gcagcctgag tggttgccgg
     1561 ctgctcctgc tgtacggcca cgggagcagg agccgcagtc ttgcccagtt tggtgtcaat
     1621 ctgctttcgc agggttttca ctcttccgtc caccagtttc aacttggtta aggagtcttg
     1681 atcgttgttt ttctgcgcat cggacatcat tgtctcgacc ttcttcaaac tagcatctgc
     1741 ctcgtctgtc tttccctgaa aaaacagccg ctgcgcttca ccaatcagct tgccggcatc
     1801 tgcagcggtt tctgccaaag atccggagac gaacagcaca aacatgaagc ccacaacagt
     1861 cacaaccaga caacgacgac ccataaacat gatcaaacct ccaagatttc ttttgaacac
     1921 acaccgttcc caggtaagtg ggtgtgtggt tcggattcct tttcctcgcg ctggggtctg
     1981 acttcagttt cagactacgc cttttgttca gattcccaga ctcgacaaac ccttatcttc
     2041 taaagaaggc tatcctgaag catgatagtg taaactgctc tgtctcgcaa ccagatgagc
     2101 gcgacgatac aacggacaag gaagtttttg agaacagtga acagctattt cttcttgtgc
     2161 gtccgctttc gtttgccctt ggggctggta ctggcagcgt cggatagctg gcgggaaggt
     2221 ttggttcttc ggatgacggt cttgacccct ggaataggaa tcagtctgtt gatgacccgg
     2281 ttgacatgat ccacgtccac cacatccatc atgaacacaa gtttcgccgt gccatcatcc
     2341 tgagagcgcg cgaccgagcc tcggatattg gttcccgttg aggcaatcac gctggtgatg
     2401 tctgagagca ggcgctccct gtcagttgct tccaccccga tctccacctg gaatgactcc
     2461 gattcgcccc gatcccagta aagaggcagc aaccgcgctt cgctgacatt ggcagatgca
     2521 aggctctgac attccttggc atggatcgaa atgcctcgat tcttggtgac aaaaccaacg
     2581 atcggatcac cgggcagagg attgcagcac aacgcgcgac gataggagta gccgtcaatg
     2641 tcccgaatgg ggatcatgcc cggggtggct tccgatgcct gtttcttctg aaacaactgc
     2701 gttttgggaa gcagtcgcga gatgacatgc ttggtcagca ccttgccgaa gcccacactg
     2761 acatagagtt cgtcgagagt tcgcagatgg aaatcctcaa agactcgcgt catcttctcg
     2821 ggatcttcaa gcatcttcac agga
//
LOCUS       DAOQUC010000397         2842 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_41631_length_2842_cov_4.163258, whole genome shotgun sequence.
ACCESSION   DAOQUC010000397 DAOQUC010000000
VERSION     DAOQUC010000397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2842
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41631_length_2842_cov_4.163258"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="PKH07_07850"
     CDS             complement(<1..237)
                     /locus_tag="PKH07_07850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M24"
                     /protein_id="HNT34895.1"
                     /translation="MTHKEFDEKSRRVRSLMEREGLGAVLFTTQAGFSWITCGGRNHV
                     SLASTKGCTKVLMTRDKTYALCSLIELYRMRDEEV"
     gene            complement(1108..1506)
                     /locus_tag="PKH07_07855"
     CDS             complement(1108..1506)
                     /locus_tag="PKH07_07855"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNT34896.1"
                     /translation="MKILVVEDDDTFRELLAEFLERYGHEVSAVGKASQAQTLIDITQ
                     GDFDILISDIFLPGSTGFNVLRYVKDNFAHITTLVMTGFPNIVNSTQAIELGAYSFIP
                     KPLKLVDMISLLRRIEKMREREGTLTGVKT"
     gene            1840..2562
                     /locus_tag="PKH07_07860"
     CDS             1840..2562
                     /locus_tag="PKH07_07860"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HNT34897.1"
                     /translation="MSDFRAIVYDLDGTLVDTFQDISEAANHALVTCGRKPLSLETWK
                     RLVGYGVKHLYGCALAGGLDENGNLLGPKPTDEEIEKAIQATKEYYDKYPIVHSKPYL
                     GVEEVLEAFHREGLKQAILSNKMHAVTDLTVNRLGLARWIDPVIGESSRFPTKPDPTA
                     LKYLMQTWKVTPQQTLMVGDAETDLNTAKNAGVRVCLLTYGARSREQLQALEPDWLID
                     DFRSLRGIVLGCPPSQKTAEAL"
     gene            2562..>2842
                     /locus_tag="PKH07_07865"
     CDS             2562..>2842
                     /locus_tag="PKH07_07865"
                     /inference="COORDINATES: protein motif:HMM:NF027338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding family 9-like protein"
                     /protein_id="HNT34898.1"
                     /translation="MKSYPLTWLQDCESPVAWDTIPEIEIACFPWYEKGDKQRTWAQA
                     AISEKFLHVRFRCEDKHIYAQFTQPNDPVSRDSCVEFFFAPEPDVSICY"
BASE COUNT          674 a          738 c          725 g          705 t
ORIGIN      
        1 cacttcctca tcacgcattc gatacaactc aatcaaactg cagagcgcat acgtcttgtc
       61 ccgggtcatc aagaccttgg tgcatccctt ggttgaagca agactgacgt gattgcgtcc
      121 gccacaggta atccacgaga aaccggcctg tgtggtaaac aagaccgctc ccaagccttc
      181 acgttccatc agactgcgaa ctcgtctcga cttctcatcg aattccttgt gagtcatttc
      241 atcgctcctt gaatggcatc acacttattg gcatatcaca tcaaggatga tagtacgagg
      301 agaccaacag tgctatcgaa acagaacctt cttggtactg gctcagttaa ggagggggtg
      361 gaggtatgct tcggttgcag aggctctgcg acgctgccga agcgagaaca gcaagacagc
      421 gggcttcggt agcgttgctc cgaagactcc gctttgcttc cgagcaggtt agcacctgcg
      481 ggcgcgtggg ctatgcccac tctctcggat gcttgctcgg taaatgacac gtccctcgga
      541 ctctgtgtca ctaggagcat accttgcctt tatcacctgc ataactgagc caataccctt
      601 cttgaacatg ctgtccctgg acagatcacg atctgattca ctccccattt cccactcaat
      661 gaaaaccccc caaggttcac tgaaaccttc ggggttgatc ttcttctttt cggagtgcgg
      721 cggagtggtc ggtctaacga tgccgttgcc ttactaccca ctgcgtttgg gaccctgcaa
      781 cctgcgactg cattcggggc agaggaattt gggaggcatc tatcggtcgc ttcggctgcg
      841 tacgggcctt aatcgctcta ttcctcctga tgctgatgat ttgtttttgt taatcaattg
      901 aaatcagtcc agcatcatct tcccgaccat ccgccgtctc ctctttgtat ggactcaggg
      961 cgttgcgcca ccctggtgcg cttggagcac ttctgaatca gtcactcctt tctcccggga
     1021 tgaaacccga gctctgtggg ggtgcactct ctcggcacct gctttgccca cgccagggga
     1081 aaagtcccac ctggcagaat aaccatccta agtcttcact cccgtgagcg tcccctcacg
     1141 ctctctcatc ttctcgatac gccgcaacag ggaaatcata tccaccagtt tcagcggctt
     1201 ggggatgaat gagtatgcac ccagttcaat ggcctgtgtc gagttgacga tgttcggaaa
     1261 acccgtcatg accaatgtgg tgatgtgtgc aaagttgtct ttgacgtacc tcaggacgtt
     1321 gaacccggtg ctgcccggca aaaagatgtc cgaaatcaga atgtcaaaat ctccttgtgt
     1381 gatgtcaatc agagtctggg cctgagaggc tttgccgacg gcagagacct catggccgta
     1441 tcgttcaagg aactcagcga gcagttctcg gaacgtgtca tcatcttcaa ctactagtat
     1501 cttcatggtc tctttcctct tatacgctca tgtcaattca gtaataagca tacatcgtgc
     1561 caaagtgaaa gaactgggat tgggtcgtga tcagaaggaa aaaagcacaa aatgaagatc
     1621 tgacgatctc tacaatatat tgttatcaat aggcttaggc tctcaggcct gtcttgacga
     1681 tactgccgtc ccattcatga agaacagatt tggtggaaag cggaaagcca caagttgggg
     1741 tgtaaccaca aatgttgggg ttagttgtgg ggacttgaca aggctgtaac aaggtgcttt
     1801 gcttcctcga tctgtttgtg gaaaggaaag agaaagtctg tgagtgattt cagggcgatc
     1861 gtctacgatc tggatgggac tctggtagac acatttcagg acatttctga ggctgccaac
     1921 catgccttgg tgacctgtgg tagaaaacca ttgagtctgg agacatggaa gcgtcttgtc
     1981 ggttacggag taaagcatct ctatggatgc gctttggcgg gtggtctcga tgaaaacggg
     2041 aatctcctcg gccccaaacc taccgatgag gaaatcgaga aggcaattca ggcaaccaaa
     2101 gagtactacg acaagtatcc gattgtgcat tcaaagcctt accttggtgt tgaggaggta
     2161 ctggaggcct ttcatcgtga aggactcaag caggccattc tgagcaacaa aatgcatgcc
     2221 gtcacagatc tgaccgtcaa tagactgggg ttggctcgtt ggatcgatcc cgtgatcggc
     2281 gagtcgtctc gatttcccac caagccggat ccgacggcac tgaaatatct gatgcaaacg
     2341 tggaaagtca caccgcagca gacactcatg gtcggtgatg cagaaaccga tctgaatacg
     2401 gcaaaaaatg ccggtgtacg tgtatgcctg ttgacctatg gagcgcgttc aagggaacaa
     2461 ctgcaggcgc ttgaacccga ctggctcatt gatgattttc gatcccttcg cgggatcgtt
     2521 ctgggttgtc cacccagtca gaaaactgcg gaggcgctgt gatgaaatca tatcctctca
     2581 catggctgca agactgcgaa tcccctgtgg cttgggacac aatccctgaa atcgagatcg
     2641 cctgtttccc ctggtacgaa aagggagaca agcaacgaac ctgggcgcag gcagcgatct
     2701 cggagaagtt cctgcatgtg cgttttcgat gcgaggacaa acacatctac gcccaattca
     2761 cccaacccaa tgatccggtt tcccgggaca gctgtgtgga gtttttcttc gctcccgagc
     2821 cggatgtttc gatctgttac tt
//
LOCUS       DAOQUC010000398         2837 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_41736_length_2837_cov_3.049605, whole genome shotgun sequence.
ACCESSION   DAOQUC010000398 DAOQUC010000000
VERSION     DAOQUC010000398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2837
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41736_length_2837_cov_3.049605"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..394)
                     /locus_tag="PKH07_07870"
     CDS             complement(<1..394)
                     /locus_tag="PKH07_07870"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HNT34899.1"
                     /translation="MNHWKQITDIIDGHQRFLIVSHQYPDGDASGSCLGLLHYLCSLG
                     KQSRCVNCSPIQKVLEFLDPERVHQVYDKNTFDWSWPEVVFILDTSDVKRLGPVGDDL
                     PWDRVISVNIDHHRSSSEAIPASVSQVDA"
     gene            complement(889..2283)
                     /locus_tag="PKH07_07875"
     CDS             complement(889..2283)
                     /locus_tag="PKH07_07875"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HNT34900.1"
                     /translation="MNEIGYVSMNQPSCARGSWREVFLVAYPLILSSFSATLMHFVDR
                     LYLSWYDTRTIAAAMPGGILSFTMVCLFMGTARFTNTFVAQFYGAKDYERCAAATWQG
                     VYFALLAAVLIPLTRPLGERILTWGNHSAEIQAMERLYFRILLYGGGLEVLNAAFSSF
                     YTGRGKTLVVLWVTLIANAANAVLDYAMVFGRLGFPEMGIKGAALATVICSAIPCVIF
                     MVLMLGRENRRLYATTRFVFRKDLFWNLIRYGVPSGFNLFLDISSFTLFVILVGRLGE
                     SQLAASNIALSINTLSFFPILGISIATSTVVGQCIGARDIRAAQRTPYSALRLALTYA
                     GVFALLFGCAPDAFFRLFAQRSEGGIAFETVARHGRYLMRLVALYGLFDALNLTFCGA
                     LKGAGDTRFAMWVGLIGSWLFFVPCVYVLIHFFQANLLAVWGCMTIYAISLGVAYFVR
                     FRMGQWKSIDLIEH"
     gene            complement(2445..>2837)
                     /locus_tag="PKH07_07880"
     CDS             complement(2445..>2837)
                     /locus_tag="PKH07_07880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNT34901.1"
                     /translation="KSENKWLEIVVLLIPTHNDSPEEIQDLCGWVMDNLGPDVPMHFT
                     RFHPTYRLKDVPPTPPSTLEKAREIGMAKGLHYVYAGNVPGNPGENTYCPKCKALLIE
                     RKGFFIISNTLVGGKCPKCSEPIPGVWA"
BASE COUNT          745 a          763 c          700 g          629 t
ORIGIN      
        1 ccgcatccac ttggctgacg gatgccggaa ttgcttctga cgaggaccga tggtgatcaa
       61 tgtttacact gatcacgcga tcccatggaa gatcatcacc caccgggccg agacgtttca
      121 catcactggt gtccaggatg aaaaccacct cgggccatga ccagtcaaaa gtattcttgt
      181 cgtaaacttg atgtactctc tccggatcga ggaactcgag cactttctga atcggggagc
      241 agttgacgca tcgtgattgt tttccgagcg agcagagata gtgaagcagt ccaagacagg
      301 aacctgaggc atcaccgtct ggatactgat gactgacaat cagaaatctc tgatgtccgt
      361 caatgatatc cgtgatttgt ttccagtgat tcattgcccc gcttgtccct gcctttggtg
      421 aatcggcttt ctcttctttt ctcttttcgc tctgtctctt ctcgaccgcc actccacaac
      481 ttattcggtt caggcagctg tcattccaca acccctagtg ttgtttgatt atcagagccg
      541 attgtgaatg tcaatgattt ttttcatgaa attgatcttc tgatgaaaaa agtttcttgt
      601 gcatccgaat gtgattggtc accagatcat catgtgccgt gcgtgatcga tcgttgaaaa
      661 gcccatcaca agcctttctc agcgatcata agcacgcagt gatgatctcg aaggacaaca
      721 cgtgacttca gaacgcacca caatcgcaat caactcttcc tgcatgagac acccatgaaa
      781 tcatgtcttc gatgtgatca aacacatcaa tgatctgtcc aaacgagaac aaagatcagc
      841 aagcgtggat cgttctctgc atgagacagc agagcacgca gcgatcggct agtgctcgat
      901 taaatctatg gatttccact gtcccatgcg gaatcgaacg aagtaagcaa cgcccaaaga
      961 gatcgcatag atcgtcatgc atccccacac ggcaagcagg ttcgcctgaa agaaatggat
     1021 caacacatag acgcaaggaa caaagaacaa ccagctgcca atcagtccca cccacatggc
     1081 aaatcgtgta tctcctgccc ctttgagtgc accacagaag gtcaggttca gagcgtcgaa
     1141 gagaccatac agtgccacca gccgcatgag atagcgtcca tgtctcgcca ccgtttcaaa
     1201 agcaatcccg ccttccgagc gctgtgcgaa aagtctgaag aacgcatcgg gcgcacatcc
     1261 aaaaagcagg gcaaacactc ctgcgtacgt gagtgccaga cgcagtgcgg agtacggtgt
     1321 gcgttgcgcg gcgcggatgt cccgtgctcc gatgcattga ccaacaaccg tgctggtggc
     1381 gattgaaatc cccaggatcg gaaagaacga cagagtgttg atgctcagcg cgatgttgga
     1441 tgcggccagt tgcgattcac ccagacgtcc taccagaatc acgaacaagg tgaaagaaga
     1501 gatgtcgaga aacagattaa accccgacgg tactccgtaa cggatcagat tccagaagag
     1561 atcctttcga aacacaaatc gtgttgttgc atagagacgt cggttctcac gtcccaacat
     1621 caacaccata aagattacac atgggattgc cgaacaaatg accgttgcca gcgccgcgcc
     1681 tttgatgccc atttctggga aaccaagacg tccaaacacc atggcgtagt ccaacaccgc
     1741 gttggcggca ttggcgatga gtgtcaccca gagcaccacc agcgtcttgc ctcgaccggt
     1801 gtagaaagat gaaaatgccg cgttgagtac ttcaagtccg cccccataga ggagaattcg
     1861 gaaatagaga cgctccatgg cctggatctc tgctgaatga ttgccccaag tgaggattcg
     1921 ttcccccaaa ggccttgtca gtggaatcaa gaccgcggca agcaatgcga aatagacacc
     1981 ttgccatgtt gccgccgcac agcgctcgta gtctttggca ccataaaact gcgcgacaaa
     2041 agtattggtg aaacgggctg tacccataaa aaggcagacc atggtgaaac tgagaatgcc
     2101 acccggcatc gccgcagcga tggtgcgcgt gtcgtaccag gacagataga gccgatccac
     2161 gaaatgcata agcgttgcgg agaaactgct caaaatgagc gggtatgcaa cgagaaaaac
     2221 ctcgcgccaa ctgccacgag cacaggatgg ttgattcatg gacacatatc cgatctcgtt
     2281 cactcttctc tcccaagaga ctgcacctga cttgtcatgc taagcaggtt agcacatggg
     2341 cacgtgggct atacacatac cttcactacg cttgctcgcg aaagaaatcg gttcttccgg
     2401 agtttggctt cccgagagca ggttagcacc tgcgggcaca tgggctatgc ccacacacca
     2461 gggatgggct ccgaacactt ggggcatttg ccaccgacca aagtgttgga gatgatgaag
     2521 aagccttttc gttcaatgag cagtgccttg cacttcgggc agtaggtgtt ttcacccggg
     2581 ttccccggaa cgttgccggc atagacgtaa tgaagtccct tcgccatacc gatctcgcgc
     2641 gccttctcca gcgtcgaagg tggcgttggt gggacatctt tcaagcgata tgtcggatga
     2701 aatcgggtga aatgcatcgg aacatcaggg cccagattgt ccatcaccca accgcagaga
     2761 tcctgaatct cctcgggact gtcgttgtgc gttgggatca gtagaacaac aatttccagc
     2821 cacttgttct cactctt
//
LOCUS       DAOQUC010000399         2836 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_41757_length_2836_cov_3.233010, whole genome shotgun sequence.
ACCESSION   DAOQUC010000399 DAOQUC010000000
VERSION     DAOQUC010000399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2836
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41757_length_2836_cov_3.233010"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(233..2440)
                     /locus_tag="PKH07_07885"
     CDS             complement(233..2440)
                     /locus_tag="PKH07_07885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34902.1"
                     /translation="MPLSSLLIVGVLLAAASFLYSPRIVYNPLRSQFRWLLSILRMTF
                     LVLVFWLCFKPFFVRDERVQVPNRVLLLLDDSFSMTLDSGFQGTQGQTISRADFIRDN
                     ILGKHGLASQLQRRGFQCQIRSLSTGSVLQDENEYMENAPITAIGEALRDGIARTEDL
                     DTDKIVLVTDGCNNAGEDPLNVVQTARVPVFALGMGQTDQPTDVAVTGFVVPERVFRG
                     QEVGIHIELESSSDVDCATVLLSTEGNVISSATTPIRQGKAAIDFETSFEIEGVVLLK
                     AEVVPIPGESLTTNNTSERAIHVTRQLTRGLIVWGHPDWEFRFLTRALRQDKRLAVTT
                     ACAMDGRQTIVVRTAVEQDGTSDRTDSERLMVPVSQVIREETRQGLDWVLLGNLPASD
                     LGAQGLDTIAHWVSESGGNLFVTGGPKSFAAGGYSGTALEKILPVVLSGQSDYQTGQP
                     AIELTDAGREHLPFAAVASNPLPPLSGANSFQALKPGTDVLFSVAQAAEISPSIVTGR
                     SGLGRVLVFGGYGTWAWQMYPSKTQSLEMGTSDIHGRFWRSFVDYFSAEPGSSTVAIE
                     TDKSVYEQGSEAQVWVAMPPSILGASPEPQIRMNVQDPEGETDSLVLGLRGAEGGIYT
                     GRYRLRKAGVHVIEFPWAGKTSTREITAEPARLEYAQLVQNEAMLRSLAQATGGAYFT
                     GLQWERLLESIPLTHKSVRQRRFVLVGQQPWLFGLLLGLLAAEWILRRRVGLP"
     gene            complement(2480..>2836)
                     /locus_tag="PKH07_07890"
     CDS             complement(2480..>2836)
                     /locus_tag="PKH07_07890"
                     /inference="COORDINATES: protein motif:HMM:NF014872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylenolpyruvoylglucosamine reductase"
                     /protein_id="HNT34903.1"
                     /translation="QNIRSEMERICQKRWEKLPRPDVATAGSFFKNLPPRAPGEHRQA
                     IGAFLDQAGAKSLRVGDASVYPRHANVIVNDGSASAQDVLKLAHQMAVLVKERFGIIA
                     EPEVRLIGKGISWQPA"
BASE COUNT          660 a          783 c          743 g          650 t
ORIGIN      
        1 ttcgattggg atgttcgcag gaatctggta tgcatcagca gcgatcgcac ttgtgactgg
       61 aatcgtcttc atgtttgttt ttcgcagcca gtgccctgct gaaatcggca gaaagtagga
      121 atctgtccct atccgtagac gtagtctcca gtctctagga atgcttgtcg tggcagggaa
      181 gtctcttggt acgactgaga cctgaatggt caaatcggct gcttgcgaat gctcatggaa
      241 gcccgacacg tcggcgcaaa atccattctg ctgcgagcaa tccaagcaac agcccgaaaa
      301 gccatggctg ctggccgaca agaacaaacc ttctctgtcg gacagacttg tgtgtcaaag
      361 ggatactctc cagcaatcgt tcccattgta gtccggtgaa ataggcgccg ccagtcgctt
      421 gagccaatga acgaagcatc gcttcgttct ggacgagctg ggcgtactcc agccgtgcag
      481 gctcggcggt gatttcgcgt gttgatgttt tgcctgccca agggaattca atgacgtgta
      541 ctcctgcttt gcgtaaccga tatctccctg tgtagattcc cccctcggca cccctcagtc
      601 ccaagacgag agaatccgtc tctccctcag gatcctggac gttcatgcgg atttgcggct
      661 cggggcttgc acccaggatg gatggtggca tcgcaaccca gacctgcgct tctgagccct
      721 gctcatagac cgatttgtct gtctcgatcg caacagtgga tgatccgggt tcggcgctga
      781 agtaatccac aaacgatcgc cagaatcgcc catggatatc agaagtgccc atttccagtg
      841 attgggtctt ggatggatac atttgccaag cccatgttcc atagcctccg aaaaccaaga
      901 cccgtcccaa accggaacgt cctgtcacta ttgagggaga aatctccgcc gcctgagcaa
      961 ccgaaaaaag cacatctgtt ccgggtttca gagcctggaa agagttggct ccagaaagtg
     1021 gcggaagggg attggaggct actgccgcga aaggcaggtg ctctcggcct gcatccgtca
     1081 actcgatggc gggttgacct gtttggtaat ccgactgacc tgatagtaca acgggcagaa
     1141 tcttctccag tgcagtaccc gaatagccac ccgcggcgaa agacttggga cctcctgtca
     1201 caaacagatt gccaccggat tcagagaccc agtgcgcgat cgtgtcgaga ccctgggcac
     1261 ccaagtccga tgcaggaaga tttcccagca gaacccaatc gagaccttga cgtgtttcct
     1321 cccttatcac ctgtgaaaca ggcaccatca gtctttcaga atccgttctg tcagaagtgc
     1381 cgtcctgttc aacggctgtg cgaactacga tggtctgccg gccatccatg gcgcaggctg
     1441 tcgtgacggc aagcctcttg tcctgtctca atgcccgcgt cagaaaacgg aactcccagt
     1501 ccggatgtcc ccagacgatc aaacctcttg tcagttgtct ggtgacgtgg atggcgcgtt
     1561 cagaagtgtt gttggtcgtc agcgattccc cggggatcgg cacgacttca gctttcagga
     1621 gaacaacacc ttcaatctcg aatgatgttt caaagtctat ggcagccttt ccctgacgga
     1681 tcggtgtcgt cgcggaagag atgacattgc cttcggtgga gagcagtact gttgcgcagt
     1741 ccacatcact ggatgattcc aactcaatat ggatgcccac ttcctgaccg cgaaaaaccc
     1801 tttctggcac cacgaagccg gtgacggcca catccgtcgg ctgatccgtc tgtcccattc
     1861 caagagcgaa aactgggaca cgggcagttt gaacaacgtt cagaggatcc tcgcctgcgt
     1921 tattgcatcc gtctgtgacc aaaacgatct tgtctgtatc cagatcttct gtgcgggcga
     1981 ttccgtctct gagtgcctcg cctatggctg taattggagc gttttccatg tactcgtttt
     2041 catcctgaag aaccgatcca gtggacagac ttctgatttg acactgaaag cctctccgct
     2101 gaagttgtga agcaagaccg tgcttgccaa ggatattgtc tctgatgaaa tcagcacggg
     2161 agatagtctg tccttgagtg ccctggaagc ccgaatccag agtcatactg aaggaatcgt
     2221 cgaggagcag aagaaccctg ttgggcacct gtacccgctc gtcccgtaca aagaatggct
     2281 tgaaacacaa ccagaaaacc aagaccagga aagtcattcg gagaatggaa agcaaccatc
     2341 gaaactggga acgaagtgga ttgtacacaa tacgaggtga ataaagaaaa gatgcagcag
     2401 ccaaaaggac acctacgatg agaagcgaag acagtggcat atccgactcg ttccattcct
     2461 ttcgattggc cgtttctcgt caggctggct gccaggaaat ccccttgccg atcagccgga
     2521 cttccggttc ggcgatgatg ccgaagcgct ccttgacgag taccgccatc tgatgagcca
     2581 gtttcaggac atcctgggcg ctggcagaac cgtcgttgac aataacgttg gcatgcctgg
     2641 gatagactga ggcatctcca acgcgaagac tctttgcgcc ggcttgatcc agaaaggcac
     2701 caatcgcctg acgatgctct cctggagccc gcggcggcag attcttaaaa aaactccctg
     2761 ccgttgccac gtcaggtctt ggcagtttct cccaacgttt ttggcagatc ctttccatct
     2821 ctgaacggat gttctg
//
LOCUS       DAOQUC010000400         2836 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_41764_length_2836_cov_2.745775, whole genome shotgun sequence.
ACCESSION   DAOQUC010000400 DAOQUC010000000
VERSION     DAOQUC010000400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2836
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41764_length_2836_cov_2.745775"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            62..1078
                     /locus_tag="PKH07_07895"
     CDS             62..1078
                     /locus_tag="PKH07_07895"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011553361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HNT34904.1"
                     /translation="MVKNYRPVVMPRCVMCDEDTLTSEYGKFVIEPLERGYGRTLGNA
                     LRRVLLSSIQGAAITSVRIDGVLQEVYSIPGVYEDVTDVIMNLKGMVVSLHGSEPTKL
                     YLKAEGPKEITLADIQPNPKVEILNPQHHIAHLEEGGSLEMEVSIDFGRGYVPAEANR
                     TDDMPIGVIPVDSIYSPIRKVNFLVEMARVEKITDYDRLILEIWTNGTIDPRDALNYA
                     GRVLRDHLSMFTTFEQEPEKIEPEIKVSIPAANPNLFKSVEELELSVRSYNCLQTAGI
                     RTIAQLVQMSESDLLRFRNFGKKSLQEIKEILEKMELHLGMELPNPEQFERFDHASSG
                     SKRS"
     gene            1124..1471
                     /gene="rplQ"
                     /locus_tag="PKH07_07900"
                     /pseudo
     CDS             1124..1471
                     /gene="rplQ"
                     /locus_tag="PKH07_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514262.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
     gene            1623..2027
                     /locus_tag="PKH07_07905"
     CDS             1623..2027
                     /locus_tag="PKH07_07905"
                     /inference="COORDINATES: protein motif:HMM:TIGR04138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34905.1"
                     /translation="MSPSEFMKELLSRRTIYRPEAYVFVLQGMQYTQKKLEKPRHLTG
                     QEFARSLAAFARHLYGALAKDVLAEWGIKKTIDFGHIVYDLIEMGQMSKRPEDSLEDF
                     RDIYDFDDELGSGFDWSDEILAGLSRNSKQPE"
     gene            complement(2158..>2836)
                     /locus_tag="PKH07_07910"
     CDS             complement(2158..>2836)
                     /locus_tag="PKH07_07910"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNT34906.1"
                     /translation="DAFMARPVEDLRRFAQRMKEEVGLPFVVTGVTPTNLTDEKLEIL
                     LNGGLQWIRLGIQTTSERVNRNVYNRPTSFEAIKRAVEIIRKYHHRMDRVWYDFIIDN
                     PWETQEETLETLRFMLTLPRPFALCVYSLTYFPKTRIAERALQEGILNDPNSQVYRKS
                     YKNYQRTYINNLFLFFTLSRLPRWVLGLFVNRWILRLGLHHPLTACHTVLRKAKSRFL
                     QWKYRNR"
BASE COUNT          669 a          684 c          791 g          692 t
ORIGIN      
        1 taattgccct ttgtactgtc ctaagttcag aggggagttg aagaacagga ggctagttgg
       61 cttggttaag aactacagac ctgtagtgat gccccggtgc gtcatgtgcg acgaagacac
      121 tttgacgtcg gaatacggca agtttgtcat cgagccgttg gagcggggct atggccgaac
      181 tttgggaaac gctttgcgga gagtgttgtt gtcctctatt cagggagcgg cgatcacctc
      241 agtgaggatt gatggtgtgc tccaggaggt ctactccatt cccggcgtct acgaagacgt
      301 caccgacgtc attatgaatc tcaaagggat ggtcgtgagt ctgcatggat ctgagcccac
      361 aaagctctat ctcaaggctg aaggacccaa ggaaattacc ttggcagaca ttcagcccaa
      421 tcccaaagtg gaaatcctca atcctcagca ccacattgcc cacttggaag agggcggttc
      481 tctggagatg gaggtcagca tagactttgg tcggggttat gttcctgcgg aagccaatcg
      541 caccgacgac atgcccattg gcgtgatccc tgtcgacagc atctactccc caatcaggaa
      601 ggtgaacttc ctggtggaga tggcgcgagt cgagaagatt accgactacg accgtcttat
      661 tctggaaatt tggaccaatg gaaccattga tcctcgcgat gcgctcaact atgcgggtcg
      721 cgttttgcgt gatcatcttt cgatgttcac cacctttgag caggaaccgg agaagatcga
      781 gccggaaatc aaggtttcga taccggctgc caatccgaat ctgttcaagt ctgtcgagga
      841 actcgaactg tcggtgcgtt cgtacaattg tctgcagaca gcaggaattc ggacgatcgc
      901 tcagttggtg cagatgtcgg agtccgatct gttgcgtttc cggaacttcg ggaagaaatc
      961 gctgcaggag atcaaggaaa tcctggaaaa gatggaattg catctcggca tggaactccc
     1021 caatccggag caatttgaac gattcgatca tgcatcctca ggttcgaaac ggtcatagtg
     1081 ttctcgccgg agacggcggc agcccacgga ggaagaaagt agcatgagac acgggaagac
     1141 acataggaaa ctgggccgta cgacgtccca tcgtcacgct ttattgcgga acatggccac
     1201 ttcctttttt gagcatgaac gcattcggtc taccgaagcc aaggtcaaag aactcagacc
     1261 tgtggttgag aagatcatca ctcttgcgcg ccgaggcgac gttcattccc atcgcctggc
     1321 gatgcgtgta cttcggagcc gagaagtggc acacaaggtt tttgaggaga tcggtccgcg
     1381 atacaaggaa cggcctggtg gatatacgag aatcgtccgt ttgggacgcc gcactgccga
     1441 cgctgctccg atgtgtctga tcgaactcgt ctgaggttcg ttcagtcttg actattcgat
     1501 gatcgcctgt tgttttgtgg tgagtacatt tccgcgagca acaggtgatt cgtttttgtg
     1561 tatgggagca ggagaccttc gcagacttat ctctcgagtt tttcggcaaa ggagctggtt
     1621 tcttgtcacc cagtgagttc atgaaagaac ttctctccag aaggactatc tacaggcctg
     1681 aggcctatgt cttcgtcctt caggggatgc aatacaccca gaagaaactg gaaaagcctc
     1741 gccatcttac cgggcaggag tttgcgcgat cgctcgcagc ctttgcacgt cacctctacg
     1801 gtgcacttgc aaaggatgtt cttgcggaat ggggaatcaa gaaaacgatc gatttcggcc
     1861 atatcgtcta tgatctaatc gagatgggtc agatgagcaa acgccctgag gactctctcg
     1921 aggacttcag ggatatctac gatttcgacg atgagttggg ttccgggttc gactggtcgg
     1981 acgagatatt ggcgggactg agcagaaact ccaaacagcc agaatagtcc tcacgctcct
     2041 gttctgagac cagagaaagg gtatgatttc ttctcaaatg catgccttgg gggagtggta
     2101 tggcctactt ggccaccggc gcagggctct cagttgcagg acacggatac ccttacttca
     2161 tcggtttcga tacttccatt gcaggaagcg ggacttcgcc ttccgcagca ctgtatgaca
     2221 agccgtcagg ggatggtgaa gtcccagtcg aaggatccat ctgtttacaa aaagtccaag
     2281 cacccacctt ggaaggcgtg agagagtgaa gaacagaaac agattgttga tgtaggttct
     2341 ttggtagttc ttgtaactct tcctatagac ttgggaattg gggtcgttca ggatgccctc
     2401 ttggagagcg cgttccgcga tgcgggtctt tggaaaatag gtcagtgagt agacacacag
     2461 tgcaaagggt ctgggaagag tgagcatgaa gcgcaaggtt tccagagtct cttcttgggt
     2521 ttcccaagga ttgtcaatga tgaaatcata ccagacacga tccattctgt ggtgatactt
     2581 gcggatgatc tcgaccgctc gcttgatcgc ctcgaatgag gtcgggcgat tgtacacatt
     2641 ccggttcact cgctcacttg tggtttgaat gccgaggcga atccactgca gtccgccgtt
     2701 caagagtatc tccaacttct catcggtcag attggttggc gtgactccgg tcactacaaa
     2761 aggaagtccc acttcttcct tcatcctctg tgcaaacctg cggagatctt ctacgggacg
     2821 ggccatgaag gcgtcg
//
LOCUS       DAOQUC010000401         2833 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_41834_length_2833_cov_3.336573, whole genome shotgun sequence.
ACCESSION   DAOQUC010000401 DAOQUC010000000
VERSION     DAOQUC010000401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2833)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2833)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2833
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41834_length_2833_cov_3.336573"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..942)
                     /locus_tag="PKH07_07915"
     CDS             complement(<1..942)
                     /locus_tag="PKH07_07915"
                     /inference="COORDINATES: protein motif:HMM:NF012618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HNT34907.1"
                     /translation="MNRMICRMIVVHCLLLVCALGWGQETQTRVLQQCGYGMLNCAAY
                     SPNPDRPFVVTGGSSGAVIWNVLTAQPIRFLRAPGHTVSSLAIAPGGTQVLTGSKEDC
                     TARLWNAATGVVVRTFELSRSVECVAFSPDGTKVLTGSDDQTARLWDVTTGDLIRSFT
                     GHASEVRGVAFSPDGTKIVTGSDTGNPEYRGVAKLWDAATGDLIRTIEGPVESYVTSV
                     SYSPDGTKILTGGWDGVARLWNATSGDLVRNFVGLTVPVNSVAFSPDGSKVLTGSSDD
                     TAKLWDTDTGDLVRTIEGHEWYVVSVGFSPDGTRILTG"
     gene            complement(1133..1549)
                     /locus_tag="PKH07_07920"
     CDS             complement(1133..1549)
                     /locus_tag="PKH07_07920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34908.1"
                     /translation="MRISVPKIVSLLVLIWCIRSGMNAMQINVRQILAIPQRIVAFQE
                     VGQIHTLVLSHLTLASKDYVEIVLQQFCRENITGKGRDPATDPWGNPYRIWCNRYLFP
                     STFGVRLGENDRDKFLIVSGGEDGQFNNRGDIKSRP"
     gene            complement(1546..>2559)
                     /locus_tag="PKH07_07925"
     CDS             complement(1546..>2559)
                     /locus_tag="PKH07_07925"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNT34909.1"
                     /translation="QGVLILQESLLERFAAVVYLLAFVFPILILVWHVLRVLWLNYAP
                     IVRRVLSRISRLASGSSRRMQLKCEEILTCIRSVPVFGGLSEESLTRLADCLEIECYR
                     KRAPIVSQGDKGDAFYVIYQGSVAIISESDTGLETVLAVLGNNDSFGEMSLVNESPRT
                     ATALALTPVVALKLSKDVFQHFVMEHIEDKEAIVRLVQLGSFLNNTPLFEEVPTEVMY
                     QVLMHAEQREFRPGDIIVREGESGDEFFVIRSGEVRFWKDYGRANAVEIRTLTQGGHF
                     GEIALMEAGLRTATAVAETECKLVVLTRAAFFLLLQSSVTSGVMLEMTTRARLRSDME
                     LAR"
     assembly_gap    2560..2569
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2570..>2833)
                     /locus_tag="PKH07_07930"
     CDS             complement(<2570..>2833)
                     /locus_tag="PKH07_07930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34910.1"
                     /translation="LVTVFLDLSPFVPREMLRALDRLAGKSQLLENLRAFVDKKYVKR
                     VLLLSSSFQNEGYFVLFGTFAVLWVYSAYRIVSLVIEWHLSRL"
BASE COUNT          719 a          823 c          646 g          635 t
ORIGIN      
        1 tcccgtcaga attctggttc catcagggga aaatcctacg gataccacat accattcatg
       61 cccctcgatc gtacggacaa gatccccagt gtcagtgtcc caaagcttgg cagtgtcatc
      121 actacttccc gtcagcactt tcgaaccgtc aggggaaaaa gcaacagagt tgacaggcac
      181 cgtaagtccc acaaagttgc ggactagatc gccactagtg gcattccaca gtctggcaac
      241 tccgtcccaa ccgcccgtga gtatcttcgt cccgtccggg gagtaagaga cggaagtgac
      301 gtaagattcc acggggcctt caattgttcg tatcagatcc cccgttgcag catcccaaag
      361 ttttgcaacg ccgcgatact cagggttgcc tgtgtcgctt ccggtgacaa tctttgtccc
      421 gtctggggag aaggcaacgc cgcgaacttc agatgcatgc ccagtaaaac tccggatgag
      481 atcaccggtg gtgacatccc acagcctcgc agtttggtca tcgcttcccg tcaggacttt
      541 cgtcccgtcc ggcgaaaaag ccacacattc gactgatctc gaaagttcaa aggtgcggac
      601 aactactcca gtggcagcat tccagagtct tgcagtacag tcttccttac ttcccgtcag
      661 aacctgcgtt ccaccaggag caatcgccaa tgaactcaca gtgtgccccg gtgcccttaa
      721 gaaacggatc ggctgtgcag tcaaaacatt ccagataact gctcctgaac tcccacctgt
      781 tacaacaaac ggtcggtcag gattgggtga ataggctgca cagttgagca tcccatatcc
      841 gcattgctgt agcactcgcg tctgcgtctc ctgtccccat cccagtgcac ataccaacag
      901 aagacaatga acaacaatca tcctgcaaat cattcgattc atctcaaatc ctccggttgt
      961 agttgtagtg tggaacgagt ctgcgagttc cacctttcat ccttccgcgc aagcaagatt
     1021 gcgcactccc taagtccatc gttccaaaat ccgcctttca ccacacctat acgagagacc
     1081 ctcgcgactt ctttcaagat aactgcaaaa tgatatctca ccgatcttgc tttcacggtc
     1141 tgctcttgat atctcctctg ttgttgaatt ggccatcttc gccgccggaa acaatgagaa
     1201 acttgtcacg atcattttct ccaaggcgca cgccgaacgt cgaagggaaa agataacggt
     1261 tgcaccagat tctgtaggga tttccccagg gatccgttgc cggatctcgg ccttttcccg
     1321 tgatgttctc tcggcagaac tgctgaagca cgatttctac atagtctttt gaggcaagtg
     1381 tcaggtgact cagaacaagg gtatgtatct gccccacttc ctgaaaggca acaatccgct
     1441 ggggaatcgc aaggatctgt ctcacgttga tctgcatggc gttcataccc gatcgaatac
     1501 accagatgag cacaaggagt gagacgattt tcgggacaga gattctcatc gcgccaactc
     1561 catgtcactt ctgagtcttg cccgggttgt catctcaagc atgacgcccg acgttacgct
     1621 cgactgcagc agaaggaaga atgctgctcg ggtcagcacg acgagtttgc attcagtttc
     1681 tgccaccgcc gttgcggtac gaagtcccgc ctccatcagc gcaatctccc cgaaatggcc
     1741 tccttgagtc agcgttcgga tttcaactgc gttggctcgg ccatagtcct tccagaatcg
     1801 cacctcaccc gaacgaatga cgaagaactc atcgcccgac tccccttctc tgacgatgat
     1861 gtccccaggc ctgaactccc gttgctccgc atgcatcagc acctgataca taacttctgt
     1921 tgggacttcc tcaaaaagag gcgtgttgtt cagaaacgaa cccaattgta ccagtctgac
     1981 gatggcttcc ttgtcttcga tgtgttccat gacaaaatgc tggaagacat ccttgctcag
     2041 tttcagagcc acaacgggag tcaaagccag agccgtcgct gtcctggggc tttcattcac
     2101 caaagacatc tcaccgaagg aatcattgtt cccaagaacg gccagaaccg tctccaatcc
     2161 tgtatccgat tctgagatga tcgccacgga accctggtag attacataga atgcgtcccc
     2221 cttgtctccc tgagagacaa tgggcgctct cttccggtag cattcaatct cgagacagtc
     2281 cgcaagtcgt gtcaacgact cttcagaaag ccccccaaaa acaggaacgc tacgaataca
     2341 ggtgaggatt tcctcacatt tcaactgcat tcgacgcgaa gagccggatg ccagtcgcga
     2401 gatccgactg agcactcgcc tgacgattgg cgcatagttc agccagagca ctctcagcac
     2461 atgccagaca agaattagga tcggaaagac aaaggccagc aggtacacga ctgcggcaaa
     2521 acgctccaga agactttcct gcagtatgag aacgccttgn nnnnnnnnnt gaagccgact
     2581 caagtgccac tcgatgacaa gactcacaat acggtatgcg gaatacaccc aaagcacggc
     2641 aaatgtgcca aagagtacga aatatccctc attctgaaaa gacgagctca acagaagaac
     2701 ccgtttgacg tacttcttgt caacaaacgc ccttagattt tcaagaagct ggctctttcc
     2761 ggcgagtcga tccagagccc tcagcatctc tctcggaaca aagggagaga gatcgagaaa
     2821 gacagtcaca agg
//
LOCUS       DAOQUC010000402         2833 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_41838_length_2833_cov_2.966523, whole genome shotgun sequence.
ACCESSION   DAOQUC010000402 DAOQUC010000000
VERSION     DAOQUC010000402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2833)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2833)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2833
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41838_length_2833_cov_2.966523"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(81..>2833)
                     /locus_tag="PKH07_07935"
     CDS             complement(81..>2833)
                     /locus_tag="PKH07_07935"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNT34911.1"
                     /translation="EEACVEEPRHGDSDLEHGSRDRGAQKGKTRTLAKRAAVAAAVLV
                     VIALGYGSLHRKQAQTNTPASPPQKSQRTDPTDEGLIPHTNDGQSLPASPKPEPARPT
                     VNPGQQPDRPPESVSIDKIDEQLSKALQDGDDALAIQCVDRFGSIQDKNSVLRAFPIR
                     EAIKYQRIAVVSHILQVPVGADDLDGDDTLHKAAETNKVVLIEALVKNGHLDVDQKNA
                     EGQTPLVVAAVSGRKEAVTALLKLGADKNAVDKEDTTPLHAAVERHQREVVKVLLAHG
                     VDTRVRRQEKTAREWAEINGDSQIAADIARSESEKDGIYPLRPNASPTEIAARLSRAL
                     QGGDDAFAIQCVDKLGSLRDQAVVSEALPIREAIKYRRIAVVSHILQVPVGADDLDGN
                     NTLHNATETNKVALIEALVQKGHLEPDQKNADGQTPLILAAVSGRNEAVTSLLKLGAN
                     KDTADAGGMTPLHAAVEGRHAQVVTILLQEGADTEIRWEGKTARERAETKGYVEIVRL
                     FDELLSPWNKRVVSVPGMYYILKNMAVVRLTPLNNGQCQMDIAFATKRPGVAQRIRPL
                     REEKSSFDFDGHPYDVTVHEYRVERQQAVVTLKPRELVSADTGPPLSCVLIKEPAQYI
                     GETQIRLENGVRIGLADIVMRNRAPTVPVTVAFPKDSTRTLPPANLKQLECTQFSFDG
                     RDYFVDVHSLNPDSRTVTAAVRVLDRPAIGTPLETAKDIGHWDRQRLDVPGAYYILNN
                     MAVVTFRPHQAACHVVITFVSERNPVGRLVRSPRDVISFPFGGTEYSLTVHDYNVDRQ
                     QAVATLKPVDTLVSGSASFPSCELTKEPTEYAAGTQIRLDNGIELSLGKINWQNRTAR
                     LPVGVDFPKTSIRERKTWSLEALASQEFTFDNVDYFVDVHSLNPDRQTATIAVRTYNS
                     SK"
BASE COUNT          531 a          815 c          825 g          662 t
ORIGIN      
        1 gtgagcatgc tcttgagtct ggacgagatc cgatttattg tcaccatgac tccgatcaac
       61 ttgcagttgc gtcgcacggt ctacttcgac gagttgtacg tccggaccgc tatggtggcc
      121 gtctggcgat ccggattgag cgaatgaacg tcgacaaagt agtccacatt gtcgaaggtg
      181 aactcctgtg aggccagggc ctcgagtgac caggtcttgc gttctcgtat cgaagtcttg
      241 gggaagtcaa caccgacagg gagccgtgca gttctgttct gccagttgat cttcccaagg
      301 gacaattcga tcccgttgtc gagcctgatc tgtgtcccgg ccgcgtattc agtaggctcc
      361 ttcgtgagtt cgcacgaggg aaaggaggcc gagccggaaa cgagagtatc gaccggcttc
      421 agcgtggcca ccgcctgctg ccgatccacg ttgtagtcgt gaaccgtgag gctgtactcg
      481 gttcccccga aaggaaacga gatcacgtct ctcggcgatc gaaccaggcg tcccacggga
      541 ttgcgctcgc tcacgaacgt gatcacgacg tggcacgcag cctggtgggg cctgaacgtc
      601 acgacagcca tgttgttgag aatatagtat gcgccgggca catccagccg ttgcctgtcc
      661 cagtgtccaa tatccttcgc cgtctcaagc ggtgttccga tggcgggacg atcgagtacg
      721 cgaacggccg ccgtcaccgt ccggctgtcg ggattgagag aatggacgtc aacgaagtag
      781 tctcgaccgt cgaatgagaa ttgcgtgcac tcaagttgct tcagattggc tgggggaagc
      841 gttctcgtgg agtctttggg gaaggcaaca gtaacgggaa ccgtcggtgc tcgatttcgc
      901 ataacgatgt ccgcaagacc tatccgtacg ccgttttcca gccggatctg cgtctcgccg
      961 atatactgag cgggctcctt gatcagcacg catgaaaggg gaggtcccgt gtcggcggac
     1021 acgagttccc tgggcttgag cgtaaccact gcttgctgtc gctcgactct gtactcatga
     1081 acggtcacgt cgtacggatg tccatcgaag tcgaacgagg acttctcctc tcgtagcggc
     1141 ctgatgcgtt gtgccacacc tggacgtttc gtggcaaacg cgatgtccat ctggcattga
     1201 ccgttgttca gtggagtcaa tctaacgacg gccatattct tgaggatgta atacatgccg
     1261 ggcaccgaca cgactcgctt gttccacggg gacagcaatt cgtcaaacag tcgaacgatc
     1321 tccacatagc cttttgtttc cgcccgttcg cgtgcagtct ttccctccca tcggatctcc
     1381 gtgtccgcac cttcctggag aaggatggtg acaacctgtg cgtgccttcc ctcaacagcc
     1441 gcgtgcagtg gggtcatacc cccagcatcc gcagtgtcct tgtttgcccc gagtttcaac
     1501 agcgacgtca cagcctcatt cctgccagat actgctgcga gaataagagg agtctgtcca
     1561 tcggcgtttt tctggtccgg ttccagatgt cccttttgga ctagggcttc gatcaaggcc
     1621 accttgttgg tttccgtggc gttgtgcaga gtgttatttc cgtcgagatc gtccgccccg
     1681 actggtacct ggaggatgtg gctgacgacg gcgatacgtc gatatttgat ggcttcgcgg
     1741 atagggagtg cctctgagac gacggcctgg tcgcggaggg aaccgagttt gtcaacgcac
     1801 tggatggcaa atgcatcgtc cccaccttgc agcgctctgg acaaccgtgc ggcgatctcc
     1861 gtcggagagg cattgggacg cagtggataa ataccatctt tctctgattc agatcgggca
     1921 atatcggctg caatctgcga atcgccgttg atctccgccc attcgcgcgc ggtcttctct
     1981 tgacgacgaa ctcgggtgtc aacaccatgc gccaggagga ccttgacgac ctcacgctga
     2041 tggcgttcaa cagccgcatg gaggggagtc gtgtcttctt tgtcgacggc gttcttgtcc
     2101 gccccgagtt tcaacagcgc ggtcacagcc tccttcctgc cagacacggc cgcgacaaca
     2161 agaggagtct gtccctcggc gttcttctgg tccacatcca gatgcccatt cttgaccaag
     2221 gcttcgatca gaaccacctt gttggtttcc gccgccttgt gcagagtgtc atctccgtcg
     2281 agatcgtccg ccccgactgg gacctgtaga atgtggctga cgacggcgat acgttgatac
     2341 ttgatggctt cgcggatagg gaacgccctt aagacgctgt tcttgtcctg gatggaacca
     2401 aacctgtcaa cacactggat tgcgagtgca tcatccccat cttgcagtgc tttggacaac
     2461 tgttcgtcga tcttgtcgat tgagacactc tccggaggcc gatcaggctg ctgtccggga
     2521 ttcacggtag gcctcgcagg ctccggcttg ggagaagcgg gcagcgactg cccgtcattg
     2581 gtgtgtggga tcagcccctc atccgtagga tcggtgcgtt gagacttctg cgggggactg
     2641 gccggggtat tcgtttgggc ctgcttgcga tgaagcgaac cataccccaa ggcgatgacc
     2701 acgagcactg ccgcggcgac ggcggctcgc ttggcaagcg ttcgagtctt gcctttttgc
     2761 gccccgcgat ctcgtgagcc atgctccaaa tcgctgtccc cgtgacgcgg ttcctcgacg
     2821 caggcttctt cca
//
LOCUS       DAOQUC010000403         2832 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_41866_length_2832_cov_2.675909, whole genome shotgun sequence.
ACCESSION   DAOQUC010000403 DAOQUC010000000
VERSION     DAOQUC010000403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2832
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41866_length_2832_cov_2.675909"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            71..856
                     /locus_tag="PKH07_07940"
     CDS             71..856
                     /locus_tag="PKH07_07940"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNT34912.1"
                     /translation="MRKLTIVFTLLMVLALTSGCTRTQQGALIGGGIGAGTAAIWVHN
                     SSNKLSDAEGTLIGMAAGGLAGALVGDMLDEVETNKEKADLEQQISDLNSKLADKDKT
                     IDDLKREIDQLKKDLANRPEVKVDESKGKIRFTILNEVLFDSGKDKLKPEGKQVIDAV
                     ADIIKQRYADREVNVEGHTDTDPIKISGWKSNWELSAARSLSVVHYLIGNGKVAPEKM
                     SGQAFSQYRSIASNDTPEGKAQNRRAVIVVMPAEGKVVKERSE"
     gene            870..1595
                     /locus_tag="PKH07_07945"
     CDS             870..1595
                     /locus_tag="PKH07_07945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34913.1"
                     /translation="MQTVMIVSSRQQRYFCGFLLSAFALLLLGCATTRETAPRSMAPM
                     QSTEPVRVAILPPEIDASLLPENIRPESSWFVRLFWLGRGAVITSSELQKEIRLVLIS
                     YLTMGKKYELVFPVETVEEAVDRHADWVLSCKVHDARTVLLGPNRRYFYVMLTPLPTQ
                     YFIRCLTLEARLDWDIDVVSLCSQETVFKKHHRANYLKTVRYAFPKYFEPKMWRYLRY
                     EALPAHIMDTFELPQPTPTAAAD"
     gene            1702..2412
                     /locus_tag="PKH07_07950"
     CDS             1702..2412
                     /locus_tag="PKH07_07950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34914.1"
                     /translation="MRRSFILPVLLVALLSVVGCANQKSWVYSPNSYGAPTLQRDQRV
                     AVLPFTDERPNTNKNMILMYLLPIPAHGWVTYDVPEGAAMHVNSSLWVNYKPTEDYPK
                     ALAEELSRSGIFEEAFFDYKKGTSDLYVKGTILNTRYNATMISYGLSVYGPLLWIVGL
                     PVGTVHNDLSLQMTCADSTTNEVLFTKVYSAPHYRKMSWLYKLADEFNYPVMLQGIYK
                     DFMGDLVTALAAKPTPEH"
     gene            2609..>2832
                     /locus_tag="PKH07_07955"
     CDS             2609..>2832
                     /locus_tag="PKH07_07955"
                     /inference="COORDINATES: protein motif:HMM:NF012537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide reductase N-terminal alpha
                     domain-containing protein"
                     /protein_id="HNT34915.1"
                     /translation="MARKKSSASDAKEFRPAELSENALCVLQRRYLLRDKMNDIVETP
                     QDLFRRVASNMALADRKYSPKPDIDAIAER"
BASE COUNT          701 a          707 c          774 g          650 t
ORIGIN      
        1 tagtcaagag tttggagtcg tccgcccaag cgagaaaaaa aagacgaacc gaaaatgggg
       61 gtgagaaaga atgagaaaat tgacaatcgt atttacgctt ctgatggtgc tggcgctgac
      121 gtccgggtgt acccgaacgc agcagggtgc attgatcggt ggcggaatcg gtgccggaac
      181 agcagccatc tgggttcaca acagttccaa caaactgagt gacgcggaag gcacgctgat
      241 cggtatggcg gcaggtggtc ttgctggagc cttggtcggc gatatgcttg atgaggtcga
      301 gacgaacaag gaaaaggcgg atttggagca acagatcagc gatctgaact ccaagttggc
      361 tgacaaagac aagaccattg atgacctgaa gcgtgagatc gatcagttga agaaggatct
      421 tgccaaccga cctgaagtaa aggttgatga gagcaaaggc aagattcgct tcacgatcct
      481 taatgaggtg ctgttcgact ccggtaagga caagctgaaa ccggaaggca aacaggtcat
      541 tgacgcggtc gcggatatca ttaagcagcg atatgcagac cgtgaggtca atgtcgaggg
      601 tcacacagac accgacccga ttaagatttc cggatggaag tccaactggg aattgtccgc
      661 agcacgttcc ctgtccgtgg ttcactacct gatcggtaac gggaaagttg ctccggagaa
      721 gatgtccggt caggcgttca gccagtatcg ttcgattgcg agtaacgaca ctccggaagg
      781 caaagcacag aaccgccgtg cggttatcgt cgtgatgccg gccgaaggca aggttgtgaa
      841 agagcgcagc gaatgatcgg aatctgctca tgcagacagt tatgatcgtt tcaagccgac
      901 agcagaggta cttttgtggt tttctgctgt cggcttttgc tttgttgctt ctcgggtgtg
      961 ccacgacacg ggaaacggca cccagaagca tggctccaat gcagagcaca gagccggttc
     1021 gtgttgccat cctgccccct gagattgatg caagtctcct ccctgagaac attcgtcccg
     1081 agagttcctg gtttgttcgg ctcttctggt tgggacgagg ggcagtcatc accagttctg
     1141 aactccagaa agaaatccga ctggtgctca tcagttatct aaccatgggg aagaagtatg
     1201 aactggtctt tcctgtcgag accgttgaag aggcagtgga cagacatgca gactgggtct
     1261 tgtcgtgtaa agtgcatgat gccaggacgg tgcttcttgg acccaatcgg cgctatttct
     1321 atgtcatgct gacgcccctg cccacacaat acttcattcg gtgtctgact ctggaagcta
     1381 gactggactg ggacatagac gtcgtttctc tctgttccca ggaaacggtt ttcaagaagc
     1441 accatcgtgc caactatctc aaaacggttc gatatgcctt tccaaagtac tttgagccga
     1501 agatgtggcg ctatcttcgc tatgaagcgc tgcctgccca cattatggat acgttcgaat
     1561 tgccccaacc taccccaacc gcagcagcag actgatattg accacagcgt tttgcacttc
     1621 gatctggaat gcagacgcgg tttgatgtac atttgttgcg atttccagac gtttggctga
     1681 acaatccagg aggtacttgc catgagaagg tctttcattc ttccggtgtt gcttgtcgct
     1741 ctgctttctg ttgttggctg cgccaaccag aagtcctggg tgtactcacc caattcctat
     1801 ggggcaccga cgctccagag ggatcagcgt gttgctgtac ttcctttcac agatgaaaga
     1861 ccgaacacga acaagaatat gatcctcatg tatcttcttc caatccccgc acacggctgg
     1921 gtcacctacg atgtgccaga gggcgccgcc atgcatgtga actcaagtct gtgggtcaac
     1981 tacaagccaa cagaagacta tcccaaggct ttggcagagg agttgtcacg ttcgggaatc
     2041 ttcgaggaag cctttttcga ctacaagaag gggacctctg atctctacgt gaaaggcaca
     2101 attctgaaca ctcgctataa tgccacaatg atctcctatg ggctgagcgt ctatgggcct
     2161 ctcctgtgga tcgtcggtct tcctgtggga accgttcata acgacttgtc gctccagatg
     2221 acttgcgccg actcgacaac caatgaagtc ctgtttacca aggtctatag tgcgcctcac
     2281 tacagaaaga tgtcctggct ctacaaactc gccgatgagt tcaattatcc cgtcatgctc
     2341 cagggaatct acaaggactt catgggagat ctggtcaccg cattggcagc aaagccaacg
     2401 ccagagcact agagaccgag agcattcctt ggcgaagaga atgacacact cgggaaccag
     2461 tccacaggaa cggccgagcg agactctgaa gaaatgtctt cggcaattgg ctgaattcca
     2521 acggcaatac cgcaggcagt tgcccgcgct atccccagtg tggtagaagg gtatcctgag
     2581 aactggatat tcagggaggt cgagtcaggt ggcaaggaag aagtcttcag ccagtgatgc
     2641 caaggagttc cgtccggcgg agctttcaga gaatgcgctt tgtgttctcc agcgacgcta
     2701 tctgttgcgg gacaagatga acgacatcgt ggagacccct caggatctgt tccgaagagt
     2761 ggcttccaac atggcgctgg cagaccgcaa gtactctccc aaacctgaca tagacgccat
     2821 tgctgagcgt tt
//
LOCUS       DAOQUC010000404         2829 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_41913_length_2829_cov_2.666907, whole genome shotgun sequence.
ACCESSION   DAOQUC010000404 DAOQUC010000000
VERSION     DAOQUC010000404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2829
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41913_length_2829_cov_2.666907"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1295)
                     /locus_tag="PKH07_07960"
     CDS             complement(<1..1295)
                     /locus_tag="PKH07_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNT34916.1"
                     /translation="MAREVPLERVRNIGIIAHIDAGKTTVSERILYYTGRTHRLGEVH
                     DGQATMDWMEQERERGITITSAATTCFWNDHRINLIDTPGHVDFTVEVERSLRVLDGA
                     VALFCGVGGVEPQSETVWRQADRYEVPIIAFVNKMDRVGADFYNVVDLIESQLGANPV
                     PIVIPMVSDEGLIGVIDLINHVAHYYDEPDKVSEARHDEPIPEQWRTEHDRWFKHLVE
                     KASEVDEVLLEKFCADEEISSAELVRGLRRATLARTVVPVLCGAAFKNKGVKRLLDAI
                     VEFLPSPIDLPAIIGESPEGTEIERIPKDDGRLAALAFKVVADKHMGKMVYIRVYSGT
                     MQRGSYVLNSTEGKTQRVGRLMQMHANHQENREAIYAGDIGVVVGLSDTSTGDTLCDP
                     NNPIVLEAIEFPAPVISVSISPETRSDSEKLSLGLMRLA"
     gene            complement(1480..>2829)
                     /locus_tag="PKH07_07965"
     CDS             complement(1480..>2829)
                     /locus_tag="PKH07_07965"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HNT34917.1"
                     /translation="SILLAYWVRVELIIPWMPLKPGVPPLLSRYVLLALANAGVGVAV
                     FSTSGLYRERRGMARFASATRIATMVLVSHILLLALVGLYRKYTFSRIVMAFTFGFEL
                     VFAVASNAILKRVQEAMIQRGIGFFRTLLIASEQSCRDLLPRLESTHGSLHQVVGVVL
                     CDQGALPECVLGVPVTGRLEDASSWCDAEKVDRVILALPAAHHTQTLDAISVCEQKGI
                     DYRIIPDLFEMLTLRVSVGDLNGLPTITLGETPLEGWGRFAKRTMDIVLSVLFLLLVS
                     PLMLVSAALIKLTSPGPVFYTQERVGSDGRRFTIYKLRSMRSDAEGSGIGWSVKGDSR
                     ITAVGRVLRRLDIDEFPQFYNVLRGDMSLVGPRPERPYFVDQFKTNIPRYMRRHIAKS
                     GITGWAQVHGLRGDTSIAERVRYDLYYIENWSLWLDVKILFLTALDVFRRIVRGVSC"
BASE COUNT          673 a          828 c          732 g          596 t
ORIGIN      
        1 tcagcgagtc gcatcaagcc gagggaaagt ttctcgctgt cagagcgcgt ctccgggctg
       61 atgcttaccg agataaccgg tgcggggaac tcaatggctt caagaacgat ggggttgttc
      121 ggatcacaga gcgtatctcc cgttgatgtg tccgaaagcc ccaccacgac gccaatatcg
      181 ccggcgtaga tcgcctcgcg gttttcctgg tggttggcgt gcatctgcat gagtcttccc
      241 acacgctgtg tcttgccctc ggtggagttg agaacatagg aaccacgctg catggttccc
      301 gaatagactc gaatataaac catcttcccc atgtgtttgt cggcaacgac tttgaaggcc
      361 aacgccgcaa gacgaccgtc gtctttgggg atgcgctcga tctctgttcc ttcgggcgac
      421 tcaccgatga tggcaggtag atcgatcggc gaaggcagga attccacgat ggcatccaga
      481 agcctcttga cccctttgtt cttgaacgcc gccccacaca agaccggaac aacagttcgt
      541 gccagagttg cgcggcgaag tcctcgtacc agttcggcag acgagatttc ctcgtcggca
      601 cagaactttt ccagcaagac ttcatcaacc tcgctggcct tttccaccag atgcttgaac
      661 cagcggtcgt gttctgtccg ccattgttcg gggattggct cgtcgtggcg cgcttcgctg
      721 actttatccg gctcgtcgta gtaatgcgcc acgtggttga tgagatcaat caccccaatc
      781 aaaccctcat cactgaccat agggatgacg atgggaacag gatttgcccc aagctgcgat
      841 tcgatgagat cgacaacgtt atagaaatcc gcacccacgc gatccatctt gttcacgaat
      901 gcaatgatcg gaacctcata ccggtctgcc tgacgccaga cggtttctga ctggggttcc
      961 acacccccca ctccgcagaa aagcgccacg gctccgtcga gcacccgcaa cgagcgttcc
     1021 acttcgacgg tgaaatccac gtgtcccggg gtgtcgatca gattgatacg atggtcattc
     1081 cagaagcagg ttgtcgccgc cgacgtgatg gtgatcccgc gctcgcgctc ctgttccatc
     1141 cagtccatgg tggcttgtcc gtcatggact tcccccaatc ggtgtgttcg accggtgtag
     1201 tagagaattc tctcactcac cgttgtcttt ccggcgtcaa tatgcgcgat gatcccgatg
     1261 ttcctgaccc gctcaagcgg cacttccctg gccattgttc ctctctgtcc tttctgatct
     1321 acaccgtgtt tggggtattc gatcgctgaa agcaaagggc gcgtttctag cagactcctc
     1381 ttcggtctgt caagccctta acccaaaact aacgttcaac tgggcaataa agcaacgtaa
     1441 gagcaaaccg tgaaattccg gtcaaccaat cgcatctgcc tagcaggaaa cgcctcgcac
     1501 aatgcgtcga aatacatcca aagcggtgag aaagaggatt tttacatcaa gccacagaga
     1561 ccaattctca atgtagtaga ggtcatatcg gacacgctct gcgatcgagg tgtcgcctcg
     1621 caagccatgc acctgtgccc agccggttat accggacttg gcgatgtgtc gccgcatgta
     1681 tcgcgggatg tttgttttga actgatccac gaaatagggt ctttccggac gcggaccgac
     1741 aaggctcatg tccccacgaa ggacgttgta gaactggggg aactcatcaa tgtccaaccg
     1801 acgcagcacc cgaccgacag cagtgatccg agagtcgccc ttgaccgacc agccaatgcc
     1861 ggatccttcc gcatcggatc tcatcgaacg aagcttgtaa atcgtaaagc gcctgccatc
     1921 gcttccgact cgctcctggg tgtaaaagac gggacctggc gatgtcaatt tgatcagtgc
     1981 ggcggacacc agcatcagag gagagaccag aagaaggaac agcacggaca gcacaatatc
     2041 catcgttctc ttggcaaagc gcccccatcc ttccaagggc gtttcaccca gcgtgatggt
     2101 cggcaggccg ttcaagtcgc ccacgctgac acgaagtgtc aacatttcga aaagatcagg
     2161 gatgattcgg tagtcaatac ctttctgttc acacacactg atcgcatcaa gggtttgcgt
     2221 gtgatgggcg gcgggcagcg cgaggatgac tcgatccact ttctccgcgt cgcaccatga
     2281 agaagcatct tcaaggcggc ctgtgacagg aacccccagc acacactcgg gaagagcgcc
     2341 ctggtcacac aagacgacgc cgacaacctg atgcagcgaa ccgtgagtac tctccaacct
     2401 cggaagcagg tcgcggcagg actgttcact ggcgatgaga agtgttcgaa agaaaccgat
     2461 gcctctttgg atcatggctt cctgcacgcg cttcaagatg gcattggaag ccaccgcgaa
     2521 aaccaactca aacccaaatg tgaacgccat cacaattcgg gagaaggtgt atttccgata
     2581 aagacccaca agcgccagca gaagaatgtg gctcacaaga accatcgtgg caattcttgt
     2641 cgcactggca aaccgcgcca tgcctcttcg ttcccggtaa agtcccgaag tcgagaagac
     2701 ggcaacaccc acacctgcgt tggcaagtgc caagagcaca taccgactca acaggggagg
     2761 cacacccggc ttgagaggca tccacgggat gatcaactca actcgaaccc aataagcaag
     2821 cagaattga
//
LOCUS       DAOQUC010000405         2827 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_41944_length_2827_cov_3.439394, whole genome shotgun sequence.
ACCESSION   DAOQUC010000405 DAOQUC010000000
VERSION     DAOQUC010000405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2827
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41944_length_2827_cov_3.439394"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..573)
                     /locus_tag="PKH07_07970"
     CDS             complement(<1..573)
                     /locus_tag="PKH07_07970"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HNT34918.1"
                     /translation="MMQRTQRVVLINANRMKPPIAPIGLDYVGSALQAQGFDVVLADL
                     TFATDIEGELDAVFEREPLAVGISVRNIDDSYLASSAFFLDEVRDLVTQIRARTQSPI
                     VFGGVGFSISPQEALEFCQGDFAIRGDGELALPMLLHRIRDGGEPSDVPGLLSRTDGR
                     VHVGTSPAFFDLDSVFLSSRSLVDNGRYFRE"
     gene            complement(592..1854)
                     /locus_tag="PKH07_07975"
     CDS             complement(592..1854)
                     /locus_tag="PKH07_07975"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNT34919.1"
                     /translation="MMFRNRLCAVVTVLSVMCILAPNALTQEQPISATPEPENLRRDL
                     ETFQRSFIEIKRRCIYERSARELLQSALKGMVEDLKDPYSSYLSNETANKMKSLLQEE
                     QSELTGIGVQMGYEDSQLKVIAPIQDSPAWRAGIQAGDRITQVDGVVVENMDDAVSRI
                     TGEKGTAVKITVIRSGLKEPIEFNLVREPITLKNVNREVICPPIGYVRLASFSNRSRT
                     EIIEALKFLEVSGCTAFILDLRSNPGGLLEAAIGVTDLFLEKGLLITYTVPRDEAGKK
                     EWYASGQTDYSKQPLVVLINRGSASASEIVTGALKDHHRATVIGEKSFGKGSVQEIID
                     LPDGSALKLTVAKYHTPSGICIHEMGIEPDITVAGEQESSAGTEKPSDVLTSLHERWH
                     FEERILKGLQRDPTLLAAVNKLSQQIQP"
     gene            complement(1867..>2827)
                     /locus_tag="PKH07_07980"
     CDS             complement(1867..>2827)
                     /locus_tag="PKH07_07980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34920.1"
                     /translation="AIPLDSSLFSSASLELGRLLIRLERWNEASAVLQQVAWIPLEAT
                     ATVGQLPLPLQRQRALSPDAVQSSILLAQAYLHLKKTSDASNTLGGLLKSSWQFLPED
                     KARIHLLLGHCFSQLGSLDKAQKEYEASMAAQDETTRQKARFYLAEMLFWHGEIKQAQ
                     QAYREILEGDYSRECVNDVLRRLALYTVTSEDGLKKYAQADFFAWQGRLDDAVNGFRE
                     IAAKHSGSDLSVWALLRAAEIQQESGKAEVARVEYERLLTMTENVTLLGSIQMKLAST
                     AQESQTLNKKMEEIVLQVPDSIFADLARLRLQELTTPTALPAN"
BASE COUNT          630 a          796 c          735 g          666 t
ORIGIN      
        1 ctcacgaaag tagcgtccgt tgtccaccaa agaccgtgat gagagaaaca ctgaatccag
       61 atcgaaaaaa gcgggagagg ttcccacatg cacccttcca tcggtacggg aaagcaaacc
      121 aggcacgtct gaaggttcac ccccgtcgcg aatacgatgc agcaacatcg gcagcgccag
      181 ttcgccatcg cctctgatgg cgaagtctcc ctggcagaac tcaagcgcct cctgtggcga
      241 aatagagaag ccaacgcctc caaagactat gggtgactgt gtccgtgctc tgatctgagt
      301 gaccagatct ctgacctcat cgaggaaaaa cgcagaactc gccaggtaag agtcgtcaat
      361 gttacgtacc gagatcccga cggcaagggg ctcgcgctcg aaaacggcgt caagttcccc
      421 ttcaatgtct gtggcaaagg tcagatccgc cagaaccaca tcgaatccct gagcctggag
      481 cgccgaacca acgtagtcca aaccgattgg agcgattggt ggcttcatcc ggttggcgtt
      541 gatcaagacg actctttgtg tgcgttgcat catgcgattg cctcatacgg gttacggctg
      601 gatctgttgt gagagtttgt tgacagctgc gagcagcgtt ggatctctct gaagcccctt
      661 gaggatacgc tcttcgaagt gccatcgctc atgcaaggac gtcagaacat ccgagggctt
      721 ttccgttccc gctgacgatt cctgctcgcc tgccacggtg atgtccggtt cgatgcccat
      781 ctcgtgaatg cagattccag atggcgtgtg atacttggca accgtcagtt tcagggcgga
      841 accatcgggc agatcaatga tttcttgaac ggaacccttt ccgaaactct tctctccaat
      901 gactgtggcg cgatggtgat ccttcagcgc tcctgtgacg atctccgagg cgctggcgga
      961 accccgattg atgagcacga caagcggctg cttgctgtag tcagtctgtc cgctggcgta
     1021 ccactctttc ttgcccgctt catcgcgagg tactgtgtag gtgataagga gtcccttttc
     1081 gagaaacaga tccgtgaccc caatggccgc ctcaagcaaa ccgccaggat tgcttcgaag
     1141 atccaggatg aatgccgtgc aaccggaaac ttccaggaac ttcaatgctt caataatctc
     1201 ggtgcgactt cgatttgaaa aggaggccag tcgcacatat ccgatgggag gacagatcac
     1261 ttctcggttg acattcttca atgtgatcgg ctctcgcaca aggttgaact caatcggctc
     1321 cttgagtccc gaacggatga ccgtaatctt cacggcagtg cccttctccc cggtgattcg
     1381 agaaacggcg tcgtccatgt tctccacaac gactccatcc acttgggtga ttctatcgcc
     1441 agcctggatt ccagcgcgcc aggcgggaga atcctgaatc ggggcgatca ccttcaactg
     1501 ggaatcctca tatcccatct gcacgccaat gcccgtcaac tcgctctgct cctcttgaag
     1561 caaagacttc atcttattgg cagtttcatt tgagaggtac gatgagtagg gatctttcag
     1621 atcttccacc atacccttca gggcgctctg caggagttcc cgagcggagc gttcatagat
     1681 gcagcgcctc ttgatctcga tgaacgatct ttggaacgtc tccagatccc ttctaaggtt
     1741 ttccggttcc ggagtggctg atattggctg ttcttgagtg agcgcattgg gcgccaggat
     1801 gcacatgaca gagaggacgg taaccacagc acatagtcgg tttctgaaca tcatgcttct
     1861 ctttcctcag tttgctggaa gggctgtcgg cgttgtcagt tcctgaagac gcagtcgtgc
     1921 caagtctgcg aagatcgagt cgggaacttg caggacgatc tcctccatct tcttgttgag
     1981 ggtctgactt tcctgtgccg tgctcgccaa tttcatctgg atggatccga ggagagtcac
     2041 gttttccgtc atcgtgagca gtcgctcata ctcgactctg gcaacctcgg cctttcccga
     2101 ctcctgctga atctctgcag cgcgcaacaa tgcccataca gaaagatcag aaccggaatg
     2161 cttcgctgcg atctcccgaa agccattgac ggcatcgtcc aagcgacctt gccaggcaaa
     2221 gaaatccgcc tgtgcgtatt ttttcagacc gtcttccgat gtcaccgtat agagcgcaag
     2281 tcgtcgcaag acatcgttga cacattctcg tgaatagtcg ccttcaagaa tctcccgata
     2341 ggcctgttgt gcctgcttga tctcgccatg ccagaaaagc atctctgcaa gatagaatcg
     2401 tgccttctgg cgagtggttt catcttgcgc cgccatggag gcctcgtact ctttctgtgc
     2461 cttgtcaagc gagcccagct gcgaaaaaca atgcccaagc agcagatgaa tcctcgcttt
     2521 gtcctcgggt aaaaactgcc aggaactctt gagcaagccg cccagcgtat tgctggcatc
     2581 cgaagtcttc ttcagatgga gataggcctg tgcgagcagg atgctcgact gtactgcatc
     2641 cggagacaag gctcgttgcc gttgcaacgg gagtggcaat tggccaacgg tggctgttgc
     2701 ctcaagggga atccatgcca cttgttgaag aaccgcgctt gcttcgttcc aacgctccag
     2761 acgaatcagc aaccttccca gttccagtga ggcactggag aacaggctgc tgtccaaagg
     2821 aatcgct
//
LOCUS       DAOQUC010000406         2822 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_42062_length_2822_cov_2.756053, whole genome shotgun sequence.
ACCESSION   DAOQUC010000406 DAOQUC010000000
VERSION     DAOQUC010000406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2822
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42062_length_2822_cov_2.756053"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="PKH07_07985"
     CDS             <1..306
                     /locus_tag="PKH07_07985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34921.1"
                     /translation="GCDRADQIDVNGVSAGDHISGMLTQASESTLVVSNLMGNSLLRM
                     AELMDVAGLCIAGGTLPEQEFIESAQERGIILIASPYELFETCGRLYERLKAEGALV"
     gene            315..719
                     /locus_tag="PKH07_07990"
                     /pseudo
     CDS             315..719
                     /locus_tag="PKH07_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012269444.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            780..1862
                     /locus_tag="PKH07_07995"
     CDS             780..1862
                     /locus_tag="PKH07_07995"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HNT34922.1"
                     /translation="MCKECLRSCPTRAVRVRNGSPQILEHLCIDCNACIGECPTDALS
                     IKSAFNTQSSLRGTLVVSGAFLAQFGTAFPADVVLQALKALGFERIHILESWSLGIQQ
                     ASVEYAHRQTESQTVIVPTCPAVLNLIQLRFPSLIPHVAPFLTPLESAEAMLHTDKPA
                     FMAFCPSQRSRLREHKVYDERILIPSDLRSRVLTWLNANAGIELLSQRGSNVESDSEI
                     LSVCGLEHVMGVLNDIEDGLLRDIGVVELLGCDQGCFGSPLLSEDCYVAANRWRRCGA
                     CTVVHPAVPRLEPLVARAGIRLDENMLRAIMKLKQMQEVRSMLPGRDCGVCGAPTCDD
                     LAEDVVLNRADMAQCAYLGQQGDQRR"
     gene            1859..2206
                     /locus_tag="PKH07_08000"
     CDS             1859..2206
                     /locus_tag="PKH07_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine kinase"
                     /protein_id="HNT34923.1"
                     /translation="MIVRQLVEQLDLESLTPELDASLDVSRGYVSDMLSDVLANAPQD
                     GVLVTIQLHLNVIAVAIHAGLKAVIFTSGRRPEENVIQKACEEQIQLFVSRDCSFDLV
                     GRLYELGLRSGDA"
     gene            2199..>2822
                     /locus_tag="PKH07_08005"
     CDS             2199..>2822
                     /locus_tag="PKH07_08005"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HNT34924.1"
                     /translation="MLRLWSADLHIHTALSPCAEEEMTPPAIVEAAVAADLALIAICD
                     HNSMENVEAVQDASEGESLTVLAGMEITTAEEVHVLGLFPNTDAARSVGRLIRETLPL
                     GEKEGRLVQCQWLLNAAGEQIGWEMGMLSSASGFTLKEVVEMVHASAGLAVASHVDRP
                     SFSVTSQLGFIPDDVDFDALEISSLAHADWRKKHPMSLGVKRPILFSS"
BASE COUNT          596 a          695 c          863 g          668 t
ORIGIN      
        1 ggatgcgatc gagccgatca gatcgacgtg aacggcgtat ctgcgggaga tcatatcagt
       61 ggcatgttga ctcaggcttc cgaaagcacg cttgttgtca gcaacttgat gggaaacagt
      121 ctgcttcgga tggccgagtt gatggatgtc gccggtctgt gtatcgccgg tggtacgctg
      181 ccggagcagg agtttatcga gtccgcccaa gagcggggaa tcatcttgat cgcatcgccc
      241 tatgagctgt ttgaaacgtg tggcaggctc tacgagcgac tgaaagccga aggtgcgttg
      301 gtgtgacaga tccgatcatc ttccagatta aggcagggga ctatgacggc ggcgggaaag
      361 cctcgagggc gctcaaggag catctgaaga aggttggcgc cgacggcaag gcagtccgcc
      421 gtgccatgat tgcctcttac gaagcagaga tgaatgttgt catccattct gtcgggggac
      481 agatgacaat tcagatagag ggggatcggt tgagtgtcga agttaaggat gaaggccccg
      541 ggatcgctga tctcaacctg gccatgcgtg aaggctactc cacagcaccg gcaaaagcgc
      601 gtgagtacgg atttggcgca gggatggggt tgccgaacat caaacgcaat tcagatgagt
      661 tccatattca gtcccaagtc ggagaaggga cgacacttca gttcaaagtc ttgctcaagc
      721 aacccgaact gagtgcgggc acgcctcact ctgttcacat tcgcgcagcg ctctgtcgct
      781 tgtgcaagga atgtcttcgg tcgtgtccaa cacgagcagt ccgggttcgc aatggttcac
      841 cacaaattct ggagcatctc tgcatagact gcaatgcctg tatcggtgaa tgtccgaccg
      901 acgctttgtc gatcaagtct gcttttaaca cgcagtcttc cctcaggggt acattggttg
      961 tatcgggtgc atttctcgcg cagttcggaa cggcattccc cgccgacgtc gtgcttcagg
     1021 ctttgaaagc gctggggttt gagaggatac acattctgga gagttggtct cttggaattc
     1081 agcaggcgtc cgtggagtac gcccatcggc agaccgaatc ccagacagtc attgtgccca
     1141 catgtccggc ggtcttgaac ctaatccagc tgcgctttcc ttcgctgatt cctcatgtag
     1201 caccgttctt gacaccactc gaatctgccg aggcaatgct ccatacggac aagcctgctt
     1261 tcatggcatt ctgtcccagt cagcgttcac ggctgaggga acacaaagtg tatgacgagc
     1321 gtattctgat tcccagcgat ttgcgcagtc gagttctcac ctggctgaat gcaaatgccg
     1381 gcatagagct attgtcacag agagggtcga atgttgaatc ggattcagag attctaagcg
     1441 tttgtgggct ggaacatgtg atgggggttc tcaacgacat cgaggacgga ctcttgaggg
     1501 atattggagt tgtggagttg cttggctgcg accaaggctg cttcggttcg ccactgttga
     1561 gtgaggactg ttacgttgcg gccaatcgtt ggcgtcgttg cggcgcgtgt accgtcgtcc
     1621 atccggcagt cccgcgactc gaacccttgg tggcacgagc cggaatcagg ttggatgaga
     1681 atatgcttcg ggcgatcatg aaactcaaac agatgcagga ggttcgttcg atgcttccag
     1741 ggagagactg tggtgtctgc ggtgctccca cctgtgatga cctggcagag gacgtggttc
     1801 tgaaccgggc agacatggcg caatgcgcct atcttgggca acaaggagac caaaggagat
     1861 gatcgtgcgg caactggttg agcaattgga tctggagtcg ttgacgcctg agttggatgc
     1921 gtcactcgat gtttccaggg gctacgtttc ggacatgctg agtgatgtgc ttgccaacgc
     1981 tccgcaggat ggtgttctgg tgaccattca gcttcatctg aatgtgatcg cggtggccat
     2041 ccacgccgga ctgaaagcgg tgattttcac atcggggcgc cgtccggaag agaatgtgat
     2101 tcagaaggcg tgcgaagagc aaattcagct ctttgtgtct cgcgattgtt ccttcgactt
     2161 ggtgggacgt ttgtatgaac tgggtctgag atcgggcgat gcttagactc tggagtgccg
     2221 atcttcatat ccacacagcg ctgtctccct gcgccgagga agagatgact cccccagcca
     2281 ttgtggaagc ggccgttgct gctgatcttg ctctgatcgc catctgtgat cacaacagta
     2341 tggaaaacgt ggaggcggtt caggatgcct cggaaggcga gtcgctgacc gtcctggcgg
     2401 gaatggagat caccacggcg gaagaagtcc atgtgttggg cttgtttccg aacaccgacg
     2461 cggcgaggag cgtaggacgt ctgatccgcg agactctgcc acttggggaa aaggaaggcc
     2521 gcctggttca atgccaatgg cttttgaacg cagcagggga acagatcggt tgggagatgg
     2581 gaatgctttc gtcagcgtcc ggattcactc tcaaggaagt cgtcgagatg gttcatgcct
     2641 cggccggtct ggcggttgcc tcgcatgtag acagaccttc attcagtgtc accagtcagc
     2701 tgggcttcat tccggacgat gtggattttg atgcgctgga gatatcctcc cttgctcatg
     2761 ccgattggcg aaagaaacat ccgatgtcgc tgggcgtcaa acgcccgatt ctgttttcct
     2821 ct
//
LOCUS       DAOQUC010000407         2816 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_42193_length_2816_cov_3.067005, whole genome shotgun sequence.
ACCESSION   DAOQUC010000407 DAOQUC010000000
VERSION     DAOQUC010000407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2816
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42193_length_2816_cov_3.067005"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1489)
                     /locus_tag="PKH07_08010"
     CDS             complement(<1..1489)
                     /locus_tag="PKH07_08010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34925.1"
                     /translation="MPVTSAKHSGALSTLLAVSFVVLLCLIAPWTPGVDAAMIQADFE
                     EYLRHFAQYPTRMAGSPENEACARFIEQQFRDIGLEDVTAEEFETVVPVDKGATLSLV
                     ADASAFPIHCLWPNLVRTSTLPEEGISGEIVYGGKGDYADYNGRRVQDSIVLMDFGSG
                     NNFLKARSLGAKAIVFFDNGYVNYSQSVAKFLEVPADIPRFWIDRQSAQILLDRLSLG
                     AVQVRLSARMDLERVKAWNIFGFRHRGMRGLSVTLDRTTNRRLSEMLKSDGLDLADKT
                     FLLDDSKSVADVLKRLQLKSPSGVVLTFDRSTHEEIQNQVADRFQHVLVEDRSLRNRI
                     LKKTEFWKLIALNGYYDSSSVVPSLAPGAESASSVSSLLALAKHLDPSETDYSLMFLA
                     SGNHFGGLRGTSEFLYWHTRSRKEFAREVKPEQSIPIQLFVGLDLSSANDQVATTAMG
                     CYFYGWLHHRIWDINKNKLASYPKLFDEFIDRRADWKTEDLEDPIR"
     gene            complement(1560..2285)
                     /locus_tag="PKH07_08015"
     CDS             complement(1560..2285)
                     /locus_tag="PKH07_08015"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNT34926.1"
                     /translation="MGSLDMIRSCAGSSSAGSFRRLMQIHGRRRFTSYLTLSLWLFLM
                     LVTEVPAQESSPESFDDLVRRGDALMDARKAGDALSLWEQARIQRPDDFDLRIRIGIA
                     RSLLGEWRKAENEFRYVLETNPNNTKALFNLALVFYNQGDYERTKQYLDDLSALDPKY
                     PELNFHQGLLLEIGGDVDGAREYYIQEVNINPSCAKAWERLYARGKKEDEGPSPERCL
                     ALGGVCFLAGLLLLLYHRRKIVS"
     gene            complement(2339..>2816)
                     /locus_tag="PKH07_08020"
     CDS             complement(2339..>2816)
                     /locus_tag="PKH07_08020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34927.1"
                     /translation="SSEPSPFDSGEPSMDGGTRPGIWISDTHCNVFQAGVYLQNVLQG
                     IISNMLIYKAPDSSEDCAGVRLSRCSDLKMHHSIFVSQSRKGSFVGLLTENGTVRCQI
                     SDNTFEWCNESIHLGMGTSGCHVWDNQVQGGGSGVCVDEGERNRFRALGVDSWGSV"
BASE COUNT          722 a          808 c          668 g          618 t
ORIGIN      
        1 tccgaatggg atcttccaga tcctcagtct tccagtcagc ccgccgatca atgaactcat
       61 caaagagttt cggataagag gcaagtttgt tcttgttgat atcccagata cgatgatgca
      121 gccatccgta gaaataacat cccatggctg tggtggcaac ctgatcgttg gcgctgctga
      181 ggtcaagacc cacaaacagc tgaatcggaa tgctctgttc tggcttgact tcccgcgcga
      241 actccttccg acttcgcgtg tgccaataga gaaactccga agtcccacgc aggccaccaa
      301 agtgattgcc cgatgcgaga aacatcaacg agtagtccgt ctccgacgga tccagatgct
      361 tcgccaacgc cagcaagctg ctgaccgatg aggcgctttc agctcctggt gccagcgacg
      421 gcacaacaga tgaagagtcg tagtagccat tgagcgcgat gagtttccaa aattccgtct
      481 tcttcagaat cctgttacga agcgatctgt cttcgaccaa cacatgctga aatctgtcag
      541 caacctggtt ctgaatctcc tcatgagtgg atctgtcaaa cgtcaacacg acgccggagg
      601 gactcttcaa ctggagccgt ttgagaacat cggcaacgct cttgctgtcg tccaacagga
      661 aagtcttgtc tgccaaatcc agtccatctg acttcagcat ttcactcaaa cgcctgttgg
      721 ttgttcgatc cagcgtaacc gaaagaccgc gcataccgcg gtgacgaaaa ccgaagatgt
      781 tccatgcctt gacccgctcc aaatccatcc gagcactcaa acgaacctgc acagcaccaa
      841 gggagagtct gtcgagaaga atctgcgcac tctgccggtc aatccaaaaa cgcggaatgt
      901 ccgccggaac ttcgagaaac ttcgccacag attgtgaata gttcacataa ccgttgtcga
      961 agaatacgat ggcctttgcc cccaaagacc gtgccttgag aaagttgttc ccactgccga
     1021 agtccatcaa gacgatgctg tcttggactc tcctcccatt gtagtcagcg tagtctcctt
     1081 tgccaccata gacgatctct ccagagatcc cctcttcagg cagagtgctg gtgcggacga
     1141 gatttggcca gagacaatgg atcgggaacg cagaagcatc ggcaaccagc gaaagcgttg
     1201 cccctttgtc caccggcaca acggtctcaa actcctcagc cgtgacgtcc tccaaaccga
     1261 tgtcacggaa ttgttgctca atgaaacgcg cgcatgcctc attctctgga gaaccggcca
     1321 tgcgtgtggg gtattgggca aaatgcctga gatactcctc gaaatccgcc tgaatcatcg
     1381 cagcatcaac acctggagtc caaggagcga tcaaacacaa caacacgacg aaggaaaccg
     1441 ccaatagagt cgagagcgcg ccactgtgtt tcgcagaagt cacaggcatg atccgattgg
     1501 actccaaaaa agcatttgac tttcagagca catccgatgc cgagaggcac gtcacggctc
     1561 taggagacga tctttcggcg atggtacaac aacaagagca aacccgcaag gaaacagaca
     1621 ccgccaaggg caagacatct ctcgggcgac ggcccctcgt cttctttctt gccacgggcg
     1681 tagaggcgtt cccacgcctt cgcacagctg gggttgatgt tcacttcctg aatgtagtac
     1741 tcgcgcgcgc catccacatc tcccccaatt tcaagtagaa gcccttgatg gaagttcaac
     1801 tcagggtatt tcggatcgag cgcagacaga tcatcaagat actgttttgt tctttcgtaa
     1861 tcaccctggt tgtagaaaac caaggccagg ttaaaaagtg ccttggtgtt gtttggattg
     1921 gtctccaaca cgtaacgaaa ttcgttctca gcttttctcc attcgccaag caacgaacgc
     1981 gcaattccga tacggattct cagatcgaaa tcgtccggtc tttggatccg tgcctgctcc
     2041 caaaggctaa gtgcgtctcc agctttcctt gcgtccatca aggcatctcc tcggcgaacc
     2101 aaatcgtcaa aggactcggg cgatgactcc tgagcaggta cctcggtaac aagcatcaga
     2161 aacagccaga gagagagtgt aagatatgaa gtgaaccgtc gcctgccgtg aatctgcatc
     2221 aagcggcgga aagagcctgc tgatgaactt cctgcacacg atcggatcat atcgagactt
     2281 cccattcttg caaaaacctc aactgagact cacaagtcca gaggactgtc ctttccaact
     2341 acacggatcc ccagctgtcc acgccaaggg ctcggaatct gttccgttcc ccttcgtcca
     2401 cacagacacc cgatccacct ccctggactt ggttgtccca gacatggcaa ccagatgtgc
     2461 ccattccaag atgaatgctc tcgttacacc attcgaaggt gttgtccgat atctgacaac
     2521 gaaccgttcc gttctccgtc agtagtccaa caaaggaacc ttttcgactc tggctgacaa
     2581 agatggaatg atgcatcttg agatctgaac atcgtgagag acgaacgccc gcacaatcct
     2641 ctgaagagtc gggcgctttg tagatcagca tattgctgat gattccctga agaacgttct
     2701 ggagatagac ccctgcctga aagacattgc agtgcgtatc ggaaatccag atccctggac
     2761 gggttccccc atccatggac ggttcgccag agtcaaacgg actcggctct gacgac
//
LOCUS       DAOQUC010000408         2814 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_42232_length_2814_cov_2.664009, whole genome shotgun sequence.
ACCESSION   DAOQUC010000408 DAOQUC010000000
VERSION     DAOQUC010000408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2814
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42232_length_2814_cov_2.664009"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            264..>1892
                     /gene="argS"
                     /locus_tag="PKH07_08025"
     CDS             264..>1892
                     /gene="argS"
                     /locus_tag="PKH07_08025"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HNT34928.1"
                     /translation="MSTKAFIDETLRQVIANEGQTHGWSLPADLEVVTEVPSDPRHGD
                     LSTNIPFALAKIVRQAPLRVAQTIAEALSALPEDRMFFETVHVAPPGFLNFVVAKTWL
                     ERDLREAFQNPERYGHSQAMAGEKVMVEFVSANPTGPLHVGHARGAIAGDTLANLLES
                     QGAQVTREYYYNDGGLQMETLGRSARARYLEKLGKPFEFPEDGYRGEYMKDIAARMVE
                     QLGESLVNCTETRPFTEFAASDILKTIDKDLKDLGIKFTSWTNESEFFQMGKVLEAVE
                     RLKSAGIAYESEGAVWLRSSADGDQRDRVLVRSNGVPTYITPDIAYHINKFERGFDRV
                     INVLGGDHHGHVPGLKAALRALGFPVERLDFALIQMVSLKKGEERIKLSTRAGDFLPL
                     YEMIAEIGSDVTRYFFLMRSIDAQMTFDWDLAKSTSMENPVYYIQYLHARVCSLAKKA
                     TDMNVDWPLDPMTDLLPLELPEERTLIKAAFSYPSILASAASSLEPHRLTAFLEDLAK
                     SFHNYYTYHRIVDAEKPELTKARLYLVEAIRGIIA"
     assembly_gap    1893..1902
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1903..>2814
                     /locus_tag="PKH07_08030"
     CDS             <1903..>2814
                     /locus_tag="PKH07_08030"
                     /inference="COORDINATES: protein motif:HMM:NF016311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HNT34929.1"
                     /translation="ARRLCADQNVVGFMYALYNVLGFLYLLIAGPAYLLKVYRNRDYR
                     QGFGSRFGFFADDLAKRLAGHRWIWVHAVSVGEVIAARPVLGEIRKRFPNDKILLSTV
                     TPTGQQMARESKDADMTVFLPVDVAWWAVNRLVAMVNPKVLVIMETELWPNLIRSVSK
                     RGVPIAMINGRISDRSFKRYHKARIFLRDLLSRISFFGMQTEDDARRIMTIGAPKERV
                     HVSGSCKFDTLNIANTENLETLRALIVAGRDRRVIIAGSTHPGEEEMLLRVYEKTRRE
                     VENPLLVIAPRHIDRSPAIKTLVEQTG"
BASE COUNT          641 a          670 c          826 g          667 t
ORIGIN      
        1 gaggaagtgc aagagtactg ctccagcagg cggggtgtct tcggtgtgaa atgttctgaa
       61 ggcgggactt cgcgattgtt tggggactgg cagtcactca ggtagacaag cctttcttgt
      121 ctcgttccca agtcccttta cactcaaccc ccagtgtcct atagttcctt tgctgtcggt
      181 ggaaatactg ttgtcttgag agagaatgcg tcccgatgcg cccaagaggc gctgacgggt
      241 gcaggtaagg agtcaaacag gagttgagca cgaaagcctt tattgatgag acgctgcgac
      301 aagttattgc caacgagggg cagacgcatg ggtggtctct gcccgcggac ttggaggtcg
      361 tgacagaggt tccgtctgat ccgcgacacg gagatctgag caccaacata ccttttgcat
      421 tggcaaagat cgttcgtcag gcacctctga gagtggcgca gactattgcg gaagcgctga
      481 gtgctcttcc tgaggatcga atgttcttcg agacagttca cgtcgcccca ccgggctttc
      541 tcaattttgt tgtggccaag acgtggctcg aacgggattt gagagaggcg tttcagaatc
      601 ccgaacgcta tggacattca caagcgatgg ctggtgagaa agtcatggtc gagttcgtca
      661 gtgccaatcc caccggaccg cttcatgtgg gtcatgcccg tggtgccatc gccggagaca
      721 cactggcgaa ccttctggaa tcgcagggtg ctcaggtcac gcgtgaatac tactacaacg
      781 atggcggtct tcagatggag acccttggtc ggtcagcgcg tgcgcggtat cttgagaaac
      841 tgggcaagcc ctttgagttt cctgaagacg gatatcgcgg cgaatacatg aaagacattg
      901 cagcaaggat ggttgagcag cttggcgaga gtctagtcaa ctgcaccgag accaggcctt
      961 tcacggagtt tgctgcatcg gatattctga agacgattga caaggacctg aaagaccttg
     1021 gaatcaagtt caccagttgg acaaacgaga gtgaattctt tcagatgggc aaggtacttg
     1081 aagctgttga acgactgaag tctgccggga ttgcctatga aagcgagggc gcagtctggt
     1141 tgcgttcgtc ggcggatggc gatcagcggg atcgggtttt ggttcgcagc aatggtgttc
     1201 caacgtacat tacgcccgac atcgcctatc acatcaacaa gtttgagagg ggctttgacc
     1261 gcgtcatcaa tgtccttggt ggagaccatc atggacatgt cccaggtttg aaggcagccc
     1321 tgagagccct tggctttccc gtggaacggc tggatttcgc tttgattcaa atggtcagcc
     1381 tgaagaaggg tgaggagagg ataaaactct caacaagggc gggcgacttt ttgcccttgt
     1441 acgagatgat tgccgagatt ggcagtgatg tgactcgata cttctttttg atgcggagca
     1501 ttgatgcgca gatgaccttc gactgggatc ttgccaagag cacatccatg gagaatccgg
     1561 tttactacat ccagtatctt catgcgcgtg tgtgcagtct tgcgaagaag gctaccgata
     1621 tgaatgttga ctggcccttg gatccgatga cggatcttct gccgttggag ttgccggaag
     1681 agcgaacgct cataaaagca gcgttcagtt atcccagcat tcttgcaagc gctgcgtcgt
     1741 ctttggagcc gcacaggctg acggcgtttc tggaagatct ggccaagtca ttccacaact
     1801 attacaccta tcaccgaatt gttgatgcgg agaaaccgga gctgaccaag gcaaggttgt
     1861 acctcgtcga ggcaattcgg ggaatcattg ccnnnnnnnn nngtgctcgg cgtctctgcg
     1921 ccgaccagaa tgtagtggga ttcatgtacg cactctacaa cgttctgggt ttcctttatc
     1981 tgctcattgc agggcctgcg tacctgttga aagtctatcg aaacagagac taccgtcagg
     2041 ggtttggcag ccgatttggt ttctttgcgg atgatctggc caagagactt gccgggcaca
     2101 gatggatttg ggtgcatgct gtctctgtcg gtgaggtcat tgctgcaaga cctgtgcttg
     2161 gcgagatacg aaagaggttt cccaacgaca agattcttct ctcgacagtg acgcccacag
     2221 ggcagcagat ggcacgggag tccaaagacg ccgacatgac ggtgttcctg cctgtggatg
     2281 ttgcgtggtg ggcggtgaac cgtttggtcg ctatggtgaa cccgaaggtg ctcgtcatca
     2341 tggagacaga actctggcca aatctcattc gatctgtttc aaaacgaggc gttcccattg
     2401 cgatgatcaa cggtcgcatc tcggatcgtt ctttcaaacg ctatcataag gcgaggattt
     2461 ttcttcgcga tcttctgtcg aggatttcct tctttgggat gcagactgaa gacgacgcgc
     2521 gaagaatcat gaccatcggc gctcccaaag agcgagtcca tgtgagcggc agctgcaaat
     2581 tcgacacgtt gaacattgcg aacaccgaaa acctggagac gcttcgtgcg ctgatcgttg
     2641 cgggaagaga tcgaagggtg atcattgccg gaagcacaca ccccggggaa gaggagatgc
     2701 ttctgcgtgt ctatgagaag acgcgacgcg aagtggaaaa cccgctcttg gtgatcgcgc
     2761 caagacatat tgacaggtct ccagccatca agacgttggt ggagcagaca ggac
//
LOCUS       DAOQUC010000409         2813 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_42247_length_2813_cov_3.764322, whole genome shotgun sequence.
ACCESSION   DAOQUC010000409 DAOQUC010000000
VERSION     DAOQUC010000409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2813
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42247_length_2813_cov_3.764322"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..547
                     /locus_tag="PKH07_08035"
     CDS             <1..547
                     /locus_tag="PKH07_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012466592.1"
                     /note="back-translocating Elongation Factor EF4; binds to
                     the ribosome on the universally-conserved alpha-sarcin
                     loop; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HNT34930.1"
                     /translation="VGGIMRLAMDRRAVDKGSEYLPGNRVILYYLFPLNEVISDFYDR
                     LKSVSSGYASLDYDFFGYQAGDLVKLDILLNGDPVDAFSMICHRSKAELRGRSMAEKL
                     RKAVPRQMYDVAIQAAVGNRIIARETVKAIRKNVTAKCYGGDITRKRKLLEKQKEGKR
                     RMKKVGKVNLPQEAFRVVLDV"
     gene            565..2772
                     /locus_tag="PKH07_08040"
     CDS             565..2772
                     /locus_tag="PKH07_08040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34931.1"
                     /translation="MPTALKMLLFLLAIPTLGGATMFDMNEFLGFGVQDQRILSASEP
                     KVTFQTNTSYDPRIAIAVDAVIVHRHGVPDDDIARTLQSWADKGFCPQRMFFADSDAG
                     WHYTRGTFDGVNHEEDIELTQDGTQVLCAGVRPYMLPTEGWTAYLKTQVRNAIDGGAK
                     AIYPEEPLAHNFTGYEKSFQKAFQKEYGTPWVPSHTSPDAFFKTARLKNKLYHQLEQE
                     LQEYTQEYAAETGRDVDFYIPIHSLYSNIPASLVAPLGTSLSITGHKGYIGQIWTGPV
                     RWALANYSDKNATFFDSAYLLYDYFVNLVRGSDLILYLLGDPVEDDPSHRWDEYDVWY
                     KECLVAKLMFPDVNTYEAMPWPDRIFLPGYSTGGGTPAPSRYRTILMSALTVLQDAPL
                     GAVHDLQGGTHGIGMLIGDTAMWQNNHQPKLDPLFGILMPLLQQGLPVCSVPAERLHD
                     KGYMDLFQMLLVSYDAWKPEQSDYHEQLAQWVKSGGVLMLFDGQDSFDDIEMFWKAEG
                     FPNPQAHLLKKLGVKYSDWKENEGKSDLRYLHHAYGKGHVIASRITPKLCSHDATVRE
                     AFLNLIRESATRYLGSDLTEAGFLSVRRGNYLVAHTFGTTQTLDGRFLDIYHEDLPLT
                     SNVALQPNCSTVLLDVSEAMQSPIPKILHATYRLMGKKETADHTTFFIQGPDGTEGCV
                     RLFAAGRKVASVQVRTKQGDAVECGVSETGDGTVLVKFPYNAEGTGFRVDWAQ"
BASE COUNT          651 a          700 c          771 g          691 t
ORIGIN      
        1 tgttggtgga atcatgaggc tggcaatgga tcggcgcgca gtggacaaag gctccgagta
       61 tcttcctggc aaccgagtca tcctctatta tcttttccca ctcaatgaag taatttctga
      121 tttctatgat cgtctcaaga gcgtttcgag tgggtatgca tctctggact atgatttctt
      181 tggatatcag gcgggcgatc tggtcaaact tgacatcctt ctgaacgggg atcctgtgga
      241 cgctttctcg atgatctgtc atcgctcgaa agccgaactg cgtggccgtt cgatggcgga
      301 gaaacttcgg aaggcggttc cgcggcagat gtatgatgtg gcgattcagg ctgcggtggg
      361 gaaccggatc atcgcccgtg agacggtcaa ggcgattcgc aagaatgtta cagccaaatg
      421 ctatggcgga gatattaccc gaaaaaggaa acttcttgag aaacagaaag aaggcaagag
      481 acgaatgaaa aaagtcggga aagtgaatct gcctcaggaa gcttttcggg ttgtattgga
      541 tgtctgacgc ttgggagaca caacgtgcca actgcgctga aaatgcttct gtttcttctt
      601 gccattccaa ccttgggagg agccaccatg tttgatatga atgagtttct tggctttgga
      661 gtccaggatc aacggattct ctccgcctca gaacccaaag tgacctttca gacaaacaca
      721 tcctacgatc cgcgcatcgc gattgcggtg gatgcggtga ttgtccatcg tcatggagtt
      781 cctgatgacg atatcgctcg gacgcttcag tcctgggcag acaagggatt ttgccctcag
      841 cgtatgtttt ttgctgattc cgatgccgga tggcactata cacggggcac tttcgatggg
      901 gtgaaccacg aagaggatat cgaactcacc caagatggca cccaggttct ttgtgctggt
      961 gttcgaccct atatgctccc gactgaagga tggacggcct atctgaagac ccaggttcgc
     1021 aatgccattg acggtggcgc gaaagcgatc tatcccgaag aaccactggc acacaatttc
     1081 acgggctacg agaagagttt tcagaaagcc tttcagaaag agtatggaac gccatgggtg
     1141 ccttcccaca cgtctccgga tgcctttttc aagacggcgc gactgaagaa caaactctac
     1201 catcaattgg agcaggaact gcaagagtac acgcaggaat atgccgctga aaccggtcgc
     1261 gatgttgatt tctacattcc gatccacagc ctctacagca acattccggc gagtcttgtc
     1321 gcgcctcttg gcacatccct ttcaataacg ggccacaaag gttacatcgg ccaaatatgg
     1381 acagggcctg ttcgctgggc attggcgaac tacagcgaca agaacgccac attcttcgat
     1441 tcggcttatc tgctgtacga ttatttcgtc aacctggttc ggggatccga tctcatcctc
     1501 tatctgcttg gcgatcctgt tgaggacgat ccatcgcatc gttgggatga gtatgatgtc
     1561 tggtataagg aatgcctcgt tgcgaaactc atgtttcctg acgtgaacac ctacgaagcc
     1621 atgccctggc cagaccggat cttcctgcct ggctacagca ccggaggcgg aactcctgct
     1681 ccgtcacgtt accgaacgat tctgatgtcc gccctgaccg ttcttcagga tgcgcctctc
     1741 ggcgcggtgc atgatcttca ggggggaacc catggtattg gaatgttgat cggtgatact
     1801 gccatgtggc agaacaatca ccaaccgaag ttggatccct tgttcgggat tctgatgcct
     1861 cttctgcagc agggactgcc ggtgtgcagt gttcccgcgg aaaggcttca tgacaaaggt
     1921 tacatggatt tgttccagat gctcctggtc tcctacgacg cctggaagcc cgaacagtcg
     1981 gattaccatg aacaactggc gcagtgggtc aagagtggtg gcgtcctgat gctctttgat
     2041 gggcaagact ccttcgacga cattgagatg ttttggaagg cggaaggatt tccgaatcca
     2101 caggcccatc tcttgaagaa acttggtgtc aagtactcgg actggaaaga aaacgaaggc
     2161 aagtcagact tgcgctatct gcatcatgct tacggcaagg gacatgtgat tgcctcgaga
     2221 atcactccca aactctgttc tcatgatgcg actgtgcggg aagcctttct gaacttgatt
     2281 cgggagagtg cgacgcgata tctcggcagt gatttgacgg aagcgggttt cctgagcgtt
     2341 cggcgcggga actatctggt tgcgcacact tttggaacga cgcagacact cgacggacga
     2401 ttccttgata tctaccacga agatcttccg ttgacgagta acgtggcgct tcagcccaac
     2461 tgcagtacag ttctgctgga cgtttctgag gcgatgcaga gcccgatccc gaagattctt
     2521 catgccacct atcggttgat gggcaagaag gagactgctg accacaccac ctttttcatc
     2581 cagggtcccg acggtactga gggttgtgtt cgcctctttg cggcaggcag aaaggttgct
     2641 tccgttcaag tcaggacaaa gcagggagat gctgtcgaat gcggcgtgag tgagactggc
     2701 gacggtacag ttcttgtgaa gttcccctat aacgcagaag ggactggatt ccgtgtggac
     2761 tgggctcaat agaccagccc tgcttttgtt gacatcttca gcgctcaagt ggt
//
LOCUS       DAOQUC010000410         2811 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_42292_length_2811_cov_3.460813, whole genome shotgun sequence.
ACCESSION   DAOQUC010000410 DAOQUC010000000
VERSION     DAOQUC010000410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2811
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42292_length_2811_cov_3.460813"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..162
                     /locus_tag="PKH07_08045"
     CDS             <1..162
                     /locus_tag="PKH07_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013272556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HNT34932.1"
                     /translation="VRIIPVIGKSVGDMVEFGGLLGSAPIMSIQPHSSERFIHRGGRI
                     PAPINSLRN"
     gene            complement(208..687)
                     /gene="ilvN"
                     /locus_tag="PKH07_08050"
     CDS             complement(208..687)
                     /gene="ilvN"
                     /locus_tag="PKH07_08050"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005010851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HNT34933.1"
                     /translation="MEHQHIISVLVQNRFGVLARVAGLFSGRGFNIDSLSVGETQEPS
                     ISRMTIVSSGDDQVMEQINKQLNKLIDVLRVTDFTDTDHVERELVLIKVSTEKKPRSE
                     VLPIVDIFRAKIVDLQPTTLTVEMTGSQDKIEAIIELLRPFGVKEIVRSGRIAIARG"
     gene            complement(689..2398)
                     /gene="ilvB"
                     /locus_tag="PKH07_08055"
     CDS             complement(689..2398)
                     /gene="ilvB"
                     /locus_tag="PKH07_08055"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007223422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HNT34934.1"
                     /translation="MSKTGAQIVVDALIKEGVEIMFGYPGGSVLDIFDALYESPIRFY
                     LPRHEQGAAHAADGYARATGKVGVCMATSGPGAMNLVTGLATAHMDSIPVVALTGQVP
                     TQLIGNDAFQEADVVGVTRPITKHNYLVKSIEELPIIMKNAFHLARTGRPGPVLVDIP
                     KDIQKMVLPKYSYPKSPDVPGYKPTVQGHPRQIKLVAEAVKDSKRPLLYVGGGAIASG
                     ASEEIYQLATKCSIPVTTTLMGLGAFPETHDLSLKMLGMHGTTYANYAVQNADLVIAV
                     GARFDDRITGRIDDFAPYAKIIHIDIDPSAISKNIRVHIPVVGDCKNVLKELNKLVES
                     TEHTEWLQLIAKWKKESPLSYKNDDKLRPQFVIEQIWELTKGDAIITTEVGQNQMWGA
                     QFYKYIKPRTWLSSGGLGTMGYGFPAAIGAKLGKPDKLVIDIAGDGSIQMNIQELATA
                     VINDIQVKIVILNNGCLGMVRQWQELFYKSRYAHTILGPDPTTLIRDGIGYIPDFVKL
                     AEAYGAVGMRVTEKKDVKKALKKAFETPKVVILEFIVNEEENVYPMVPAGASLNQMIG
                     GMA"
     gene            complement(2527..>2811)
                     /locus_tag="PKH07_08060"
     CDS             complement(2527..>2811)
                     /locus_tag="PKH07_08060"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HNT34935.1"
                     /translation="LRYWETKFTKLTPTRVGGNQRKYTRQDLELLYTIVRLLYEEGYT
                     LEGAERKLRKGWPPAKRAGASAVVGADLNVRRELVTIREQLEQVLSLLDA"
BASE COUNT          636 a          770 c          732 g          673 t
ORIGIN      
        1 gttcgcatca ttccggtgat tggcaagagt gttggggaca tggttgaatt tggaggactt
       61 ctcggatcgg caccgatcat gtccatccaa ccacactcaa gcgagaggtt catccatcga
      121 gggggcagaa tccccgcacc gataaacagt ttgagaaact agtcgcctcg cgcatcgttg
      181 ctgcaacact acgtcaagaa cgcgacacta gcctctggca atcgcgatac gacctgagcg
      241 gactatttcc ttgacgccaa aaggtctgag taactcgatg atggcctcga tcttgtcctg
      301 gctgccggtc atctcaaccg tgagtgtggt cggttgaaga tcaacgattt tggcgcggaa
      361 aatgtcaacg atgggcagga cttccgagcg cggtttcttc tcggtgctga ctttgatcaa
      421 gaccagttcc cgctcgacat gatcggtatc ggtgaaatct gtgacccgaa gcacgtctat
      481 gagcttgttg agttgcttgt tgatctgttc catgacctga tcgtcgccac tggagacgat
      541 agtcatgcgc gaaatgcttg gctcctgggt ctcaccgacg gacaggctgt caatgttgaa
      601 acctcgaccg gagaaaagtc ccgccacgcg ggcaagaact ccgaaacggt tttgaacaag
      661 cacggagatg atatgctggt gttccatgtt acgccatacc tccgatcatc tggttcagag
      721 aagcccccgc gggaaccatg ggataaacgt tttcctcctc gttcacaatg aactcaagga
      781 taacgacttt gggagtttca aacgccttct tgagcgcctt cttgacgtcc ttcttttctg
      841 taactctcat gccgaccgcc ccgtaagctt ccgcaagttt gacgaaatcg ggaatgtacc
      901 cgataccatc tcgtatgagg gttgtgggat cgggaccgag aatcgtatgg gcatatcgag
      961 acttgtagaa caactcctgc cactgccgaa ccatgccgag acagccgttg ttgagaatga
     1021 ctatcttaac ctggatatca ttgataactg ccgttgccag ttcctgaatg ttcatctgga
     1081 tggaaccgtc gccggcaatg tcaatgacca gtttgtccgg cttgccgagt ttggcaccga
     1141 tcgcggctgg gaacccgtag cccatggtgc ccaaaccacc cgaggacagc cacgttctcg
     1201 gcttgatata cttgtagaat tgagcacccc acatctgatt ctgtccgact tcggtcgtga
     1261 tgatcgcatc acccttggtg agttcccaga tctgctcgat gacgaactgt ggccgaagtt
     1321 tgtcgtcgtt cttgtaactc agagggctct cctttttcca cttggcaatc agctggagcc
     1381 attcggtgtg ctcggtggac tcaacaagtt tgttgagttc cttcagaacg ttcttgcagt
     1441 ctcccacaac ggggatatgc actcgaatgt tcttgctgat ggcagaggga tcaatgtcta
     1501 tgtggatgat cttggcataa ggggcaaaat cgtcaatgcg tcccgtgatt cggtcgtcaa
     1561 agcgtgcccc cacggcgatg acaagatccg cattctgaac cgcatagttg gcataggttg
     1621 ttccgtgcat cccgagcatc ttgagagaca gatcgtgcgt ctcgggaaat gcgcccaaac
     1681 ccatgagagt cgtggtcacg ggaatgctgc atttggtcgc cagttggtag atttcctcag
     1741 aggcacccga ggcgatcgca ccgcccccca catacagaag aggccgcttg gaatccttga
     1801 cggcttccgc gaccagtttg atctggcgcg gatgaccctg aaccgtgggc ttgtagccgg
     1861 gaacgtccgg actctttgga taggagtact tcgggagcac catcttctga atgtctttgg
     1921 gaatgtccac cagcacaggt cccgggcgac cggttcgcgc cagatggaac gcattcttca
     1981 tgatgatcgg aagttcttcg atactcttga ccaggtaatt gtgtttggtg atagggcggg
     2041 taactccaac cacatcggct tcctgaaaag catcgttccc aatcagctgc gtcggaacct
     2101 gcccagtcaa ggcaaccaca ggaatggaat ccatgtgagc ggtcgccaaa ccggtgacaa
     2161 gattcatggc tccaggtccc gatgttgcca tacagacacc gactttgcct gtggcacgcg
     2221 cgtacccgtc ggcggcgtgc gccgcgccct gctcgtgtcg gggcagatag aaccgaatcg
     2281 gtgactcata cagtgcatca aagatgtcca gaaccgatcc acccggatag ccgaacataa
     2341 tttcaacacc ctctttaatc aaggcatcta caacaatctg cgcaccagtt ttagacaaaa
     2401 ctaagttcct ccagattctc aataatctac gtcaatttat cccagaccgt acccggaaag
     2461 tcaagaccag ggcatccagg cagctcggga tgtgaggtaa tgcctcccaa gtgcatcatg
     2521 actggattaa gcatccaaca ggctcagcac ctgctccagc tgctcacgga ttgtcaccag
     2581 ttcccgacgt acattcagat ctgctccgac gacggcactc gctccggcac gtttcgcggg
     2641 aggccagcct ttgcggagtt tccgctcggc accttccagt gtgtatcctt cttcataaag
     2701 aagccttacg atggtataga gcagttccaa atcctgccga gtgtacttgc gttggttgcc
     2761 cccaacacgg gtcggagtca gttttgtgaa cttggtctcc caataccgaa g
//
LOCUS       DAOQUC010000411         2811 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_42302_length_2811_cov_2.862482, whole genome shotgun sequence.
ACCESSION   DAOQUC010000411 DAOQUC010000000
VERSION     DAOQUC010000411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2811
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42302_length_2811_cov_2.862482"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1724)
                     /locus_tag="PKH07_08065"
     CDS             complement(<1..1724)
                     /locus_tag="PKH07_08065"
                     /inference="COORDINATES: protein motif:HMM:NF023218.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HNT34936.1"
                     /translation="MKSFCILTISSVMMFSSSRALSTDNPFGICHPWNGLGELRIGWC
                     RSGAGATAFDWGRMAPDRDTLTFKHAEGEYDNWMKPFRIKHVGLLGYTPRWASSAPEG
                     AQNSGAYPPSDLFDWSDYIERTTKHFAGRIPYYEVWNEQDIGFWKGTCNEYCELLKSA
                     YLAVKRGDPSAKVVYGGLAGVNLPFLKECYEHGVAGYFDAMAVHPYQWGDTFNDEWFY
                     RQIRDLRELMLDNGDDKEIFLTEFGWESKGTQESEDIQARLLMQAILTSLVLREEARV
                     EIVFPFTVRDWGSPSYGLIRDDDTRKPSWTAYSVLIEKMLGRRCQYRLPIKEPLRGYL
                     FSPGRQSDPTEFVLALFSTDKDIHSLEFDIDSDEVSVTSMLGESQILKSSEGKLSLSV
                     GPQPVYIQGIGAQAIRPAEPIQALDPRSEATRDLPDVWISLDIPNTSRSLFLRRGVKE
                     TICLKVSNDSEETQSLRVSMSLSNGALVKPRVMRKAITIEPKTLARLHVPIRIPSSAA
                     LEVSELTVTASVGSRSIPPLNERVRITDGFAVEFLANSQIEEEHIVDFGGSCGAPSVR
                     FGGEGWTYL"
     gene            1889..>2811
                     /locus_tag="PKH07_08070"
     CDS             1889..>2811
                     /locus_tag="PKH07_08070"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNT34937.1"
                     /translation="MMDFTKPNPNLYGIPWSTDSFNEMPYRKLGTSGFRVPNIGLGTW
                     KIGYPETGDGSRTDERTALQILDRSVEIGVTLWDTANRYNDSSGNSERIIGTWFANNP
                     DQRRNIVVATKVFGGMDGITPNHSRLSRSNILESLYACLKRLRLEYVDILYFHAYDPD
                     TPLDESLTAVEDLVQRDMVRYFAVSNFSTQQLKDAMHVASRLSSRCRIQAVQNRLDIL
                     DGEKKEHEGVLQLCAEAGVSFVPYSPLALGLLTEKYLDISKVGKGDRLYDEDALSKVA
                     SKARLNKVRQLASLAHGLGLRLNELSLVYMLT"
BASE COUNT          704 a          786 c          667 g          654 t
ORIGIN      
        1 aggtacgtcc aaccctctcc accaaagcga acgctcggag cgccgcagct tcctccgaaa
       61 tcaacaatat gctcttcttc aatttgactg ttggcgagga actcaaccgc aaagccgtcc
      121 gtgattctga ctcgctcatt caacggaggt attgagcggc tgccaaccga agcggtcacg
      181 gtgagctcag agacttcaag cgcagcggac gatgggattc gtatcggcac atgcaacctt
      241 gcaagcgtct ttggctcaat ggtaatggct ttccgcatca cccgtggctt cactaaagcg
      301 ccattggata gggacattga gacccgaagg ctctgcgtct cttcggaatc gttagatacc
      361 ttcaggcaaa tcgtctcttt cacacctctc cgcagaaaga ggctccggct cgtgttcgga
      421 atatcgagac tgatccaaac gtccggcaga tcgcgggtgg cttccgatct gggatcaagg
      481 gcttgtatcg gctctgcagg acggatagcc tgcgcgccaa ttccttgaat gtagacaggc
      541 tggggcccaa ccgaaagact caacttccct tccgaactct tgagtatctg cgactcccca
      601 agcatcgagg taacagacac ttcgtcagag tcaatgtcaa actccaaaga atgaatgtcc
      661 ttgtccgtcg agaatagcgc aagcacgaac tccgttggat cgctctgacg ccctggagag
      721 aaaagataac cgcgcagagg ctctttgata ggaagccgat actggcagcg ccgcccaagc
      781 atcttttcga tgagaacaga gtacgccgtc caactcggct ttcgtgtgtc atcatctcgg
      841 attaacccat aacttgggct tccccaatct cgaacggtga atggaaatac aatctcgact
      901 cgtgcctctt ccctaagaac cagacttgtc aggatggctt gcatcaaaag tcttgcctgt
      961 atgtcctcac tctcctgcgt tcctttgctc tcccagccaa attcagtcag gaatatctcc
     1021 ttgtcgtccc cattatcaag catcagttcc cgcaaatccc gaatctgtct gtaaaaccat
     1081 tcatcattga atgtgtctcc ccactgatat ggatgcaccg ccatcgcgtc gaagtatcct
     1141 gcaacaccgt gctcatagca ttccttcaga aaaggaagat tcactcccgc aagtccaccg
     1201 tagacaacct tcgcggacgg atcgcccctc ttgaccgcaa gatacgctga cttcagcagt
     1261 tcacaatact cattacaggt gcctttccag aagccgatat cctgctcgtt ccagacttca
     1321 tagtacggga tccgaccagc aaaatgcttt gtagtacgct cgatgtaatc agaccagtcg
     1381 aacagatccg aaggaggata agcccccgag ttctgggcac cctcaggtgc tgaggatgcc
     1441 catcgtggag tgtatcccaa caaaccaacg tgtttgatcc taaaaggctt catccagttg
     1501 tcatattcac cctcggcatg cttgaaagtg agagtatccc gatctggagc cattctcccc
     1561 caatcaaacg cagtcgctcc ggctccggag cgacaccagc cgatccttaa ctctcccagt
     1621 ccgttccacg gatggcaaat cccaaacggg ttatcagtgc tcagcgctct gctagatgag
     1681 aacatcatga ctgaactgat cgtgagtatg cagaaacttt tcatggatga actccaacgg
     1741 aaaacaggat gtccaaaggc ataaccatgc aatgactcta tcatcaacag cataggtgtc
     1801 aacgctgtgg cggctcttgt tctgctttta cattggaaca ctggtctggt actatttcat
     1861 tggaattatt tggacaaagg acggaaagat gatggacttc acaaaaccga acccgaatct
     1921 ctatggaatt ccctggtcaa cagactcttt caacgaaatg ccctatcgaa agttaggcac
     1981 ctctggcttc cgagtgccga atatcggcct gggaacgtgg aagataggat accctgagac
     2041 tggagacggc tcgcgaaccg acgaaaggac tgctctgcaa attcttgacc gctccgtcga
     2101 gattggagtg actctttggg atacagcaaa ccggtacaat gattcatcag gaaactctga
     2161 aaggatcatc gggacctggt tcgccaacaa cccggatcaa cgcagaaaca tagtcgttgc
     2221 gaccaaagtg tttggaggca tggatggcat cacacctaac cacagccgcc tttcccgatc
     2281 gaatattctc gaatccctct atgcctgcct gaaacgactt cgtctggagt acgtggacat
     2341 cctctatttc catgcctatg accctgacac acctctggac gagagtttga ccgcagttga
     2401 agacttggtt cagcgcgaca tggtccgata cttcgccgtt tcgaatttct ccacacagca
     2461 attgaaggac gcgatgcacg ttgcgtcgcg tctctcaagc cgttgccgca tccaagccgt
     2521 gcagaatcgc ctcgacatcc tggacggcga gaagaaggag catgaggggg ttcttcaact
     2581 ctgcgctgaa gccggggttt cctttgtacc ttacagcccg cttgccttag gactgctgac
     2641 ggagaaatac ttggatattt cgaaagtcgg taagggggat cgcctttacg acgaagatgc
     2701 tctgagcaag gttgccagca aggcgcgact caacaaagtt cggcagttgg cttccctggc
     2761 acatggattg ggactgagac tcaacgaact ttcgcttgtc tatatgctca c
//
LOCUS       DAOQUC010000412         2809 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_42344_length_2809_cov_2.998184, whole genome shotgun sequence.
ACCESSION   DAOQUC010000412 DAOQUC010000000
VERSION     DAOQUC010000412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2809
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42344_length_2809_cov_2.998184"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1197)
                     /locus_tag="PKH07_08075"
     CDS             complement(<1..1197)
                     /locus_tag="PKH07_08075"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNT34938.1"
                     /translation="MKRTLILIATILLIASTGWSTGDLDPVLLQKLDPSLIREFGMLS
                     QPISITGTGRPFQKETALLKPQDRMPSEPERLVNVTKTRLGNDEFLNVMIHLREDKDV
                     KTLKNLGIRVNTLSGGWATALIPKEDLVGLLRRPEVLYIHASKPVLPCMDVSVPATRA
                     SELHAPSSVKGQGVLVGIVDSGIDWTHEDFKKPDGTSRIRYIWDQEDTVGPNPSGFSY
                     GTEWTKAQIDVGNCRETDTNTHGTHVAGTVAGDGSATGNGQPANRFVGMAPEAEIVFV
                     KYSDDENSIVDGVSYIFQRAEALGKPVSVNISLATQRGPHDGTYPQDAALNNLVGSGK
                     PGKILCIAASNEGYSQYPIHAGATLATPDRGNDLYPYTAHRPWSNQQFFIAEVWYPAG
                     QTVNLRL"
     gene            complement(1220..>2809)
                     /locus_tag="PKH07_08080"
     CDS             complement(1220..>2809)
                     /locus_tag="PKH07_08080"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNT34939.1"
                     /translation="CTQQDEPAAGCHGTHVAGIAAGDGSGTGNGQPANRFKGVAHQSE
                     MMFVKYKDLDSATDGVAYMFQKAQQAGKPIAVNMSLGTIDGPHDGTTASEQLLAGVLG
                     TDVPGRSLIIAAGNSGSQTIHARSASLPAKIGDTPASYPHIVMTAYPREYVGVVPVVP
                     VEIYYPHGSNFKVSVVYPADAMYTQTAQTPWVEPGNTYEQTSIQVGPLAGGYIGILHE
                     SPNALGNTDLNRVLILMGNNSNPIIPLESYKYYILLDGAGVAIDAWHIFRDKGCLVPR
                     SAVTNPPTTMLDADDWYTVARPASMTGSICVGSYVSKTQWTDVDAQTQTQQGATLNAI
                     SGFSSRGRRRDEVQKPDLCGPGEVIISTLAANYTDAPRAHVERDGVHQKMQGTSQSAP
                     HVCGVAALMLQKNQTLTQSQIKNHLKNTAIDQGTSGWDGIWGAGKMDALAALNAVPAD
                     GATPTPTATLPPTPTSPPGNPGDVNLDGLLDTRDAIRLLRHVEGIEVLTGQALVNADA
                     NQDSAVNNADVDWILDRTVGK"
BASE COUNT          565 a          782 c          742 g          720 t
ORIGIN      
        1 caggcgcagg tttaccgtct gcccagcggg ataccagact tcagcaatga agaattgctg
       61 attcgaccag ggacgatgcg ctgtatatgg atagaggtcg ttcccccgat ccggagttgc
      121 cagggtcgca cccgcgtgaa tcggatactg gctgtatccc tcattgcttg ctgcaatgca
      181 cagtatcttc cctggctttc cacttccaac tagattgttc aaagcagcat cctgaggata
      241 cgttccgtcg tgaggcccac gctgcgttgc caaagagatg ttcactgaga ccggcttgcc
      301 aagcgcttcc gctctttgga aaatgtagct cacaccatcc acgatggagt tctcatcgtc
      361 actgtactta acaaagacaa tctctgcttc gggagccatc ccgacaaaac ggttggcagg
      421 ctgtccgttg ccggtcgctg aaccgtcgcc tgcaacggtt ccagcaacgt gtgtgccgtg
      481 tgtgttcgta tctgtctctc ggcagtttcc tacatcaatc tgtgcctttg tccattccgt
      541 tccataactg aatccagacg gattggggcc gacggtatct tcttgatccc agatgtaccg
      601 aattcgagag gtgccatcgg gcttcttgaa gtcttcgtga gtccagtcaa tgcccgagtc
      661 cacaatgccc accagcactc cctgtccttt gacgcttgat ggagcatgca actcggaagc
      721 ccgcgttgca ggaacgctga catccatgca gggtaaaaca ggttttgagg cgtgaatgta
      781 aagcacttca ggtcttctca ggagaccgac caaatcttct ttgggaatca gcgccgttgc
      841 ccatccaccg gacaacgtgt tgactcgaat gccaaggttc ttaagcgttt tgacatcctt
      901 gtcttcccga aggtggatca tgacgttcag aaactcgtcg ttgcccagtc ttgttttcgt
      961 cacgttgacc aggcgttctg gctctgaagg catcctgtct tgcggtttca gtagcgctgt
     1021 ctctttctga aagggtctgc cggtgcccgt gatgctgatc ggttgactca gcattccaaa
     1081 ctctcggatg agcgaagggt caagtttctg aagaagcaca ggatcgagat caccggtact
     1141 ccagccggtt gaggcaatca ggagaatggt tgctatgagg atgagtgttc gcttcatggt
     1201 cttgtctccc ttttgtgatc tacttcccga ccgttcggtc gagaatccag tccacgtcgg
     1261 cgttgttgac agcagagtcc tgattcgcgt ccgcgttgac caacgcttgt cctgtcaaaa
     1321 cctcaatccc ttcgacatgt ctcagcagtc gaatggcatc gcgtgtatcg agcagcccat
     1381 cgagattgac gtctcctgga tttcccggtg gactggttgg tgtcggtggg agagttgccg
     1441 tcggtgtcgg cgttgctccg tcagcgggca ctgcgttcag agccgcaagt gcatccatct
     1501 ttcccgctcc ccagatgccg tcccacccgc tcgtgccttg gtcaatggcg gtattcttca
     1561 gatggttctt gatctgactc tgcgtcagtg tttgattctt ttgaagcatc agagccgcga
     1621 caccgcacac atgcggtgca gactgactcg ttccctgcat cttctggtgc acgccatcgc
     1681 gttccacatg ggctcttgga gcgtccgtgt agttggcggc aagtgtcgaa atgatgactt
     1741 caccaggtcc acagagatcc ggcttctgaa cttcgtcccg ccgtctgcct cgactcgaaa
     1801 agcccgagat ggcgttcagt gtggctccct gttgcgtctg tgtctgagca tccacatccg
     1861 tccattgtgt cttgctgaca taagagccta cacaaatcga tcccgtcatg cttgcaggcc
     1921 gtgccactgt gtaccagtcg tcggcatcca acatcgtcgt gggtgggttg gtcactgctg
     1981 agcgaggcac aagacacccc ttgtctcgga agatgtgcca ggcatcaatg gcgacacccg
     2041 caccatcaag cagaatgtaa tacttgtaac tctccaaggg aatgatggga ttggagttgt
     2101 tccccatcag aatcagaaca cgattcaaat ctgtgtttcc cagcgcattt gggctctcat
     2161 gcaggatgcc gatgtaccca ccggcgagag ggccaacctg aatgctggtc tgttcatagg
     2221 tgttcccagg ttccacccaa ggagtctgcg cagtttgggt gtacattgca tctgctggat
     2281 agacgacgga gactttgaaa ttgctgccgt gcggatagta gatttccacc ggcacgacgg
     2341 gaacaacacc gacatactct ctcggatagg cagtcatgac aatgtgcgga tatgatgcgg
     2401 gggtgtctcc gatctttgcg ggtagacttg ctgaccgagc gtgaattgtc tggctgcccg
     2461 aattgccggc agcgatgatc aacgagcgtc ccggaacatc cgttcccaag acaccagcca
     2521 gcaactgttc cgaggcagtt gttccatcat gaggtccgtc aattgtgccc agtgacatat
     2581 tgactgcgat gggtttgcca gcctgttgtg ctttctgaaa catgtaggcc acaccatctg
     2641 tcgcactgtc caagtcctta tacttaacga acatcatctc ggattgatgt gccacacctt
     2701 tgaacctgtt tgcgggttgt ccatttcctg ttcccgaacc gtctcctgcg gcaattcctg
     2761 cgacatgggt tccatggcac cctgcagcag gctcgtcttg ctgagtgca
//
LOCUS       DAOQUC010000413         2808 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_42374_length_2808_cov_3.006175, whole genome shotgun sequence.
ACCESSION   DAOQUC010000413 DAOQUC010000000
VERSION     DAOQUC010000413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2808
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42374_length_2808_cov_3.006175"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..323
                     /locus_tag="PKH07_08085"
     CDS             <1..323
                     /locus_tag="PKH07_08085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34940.1"
                     /translation="VGEGAHRIAYLRTLVWSPEQKKAQIEIGSDDGVKVWLNDEVVHS
                     KNVGRGCTPGEDKARVVLKEGWNRLLVKVSNYTGGWGLALRIRSPKGRTLDGLEVALE
                     ESPK"
     gene            449..1849
                     /locus_tag="PKH07_08090"
     CDS             449..1849
                     /locus_tag="PKH07_08090"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNT34941.1"
                     /translation="MPDKRKNTQEKTELSRRDFFQSAAATGVGLFFAGKAFAQNESSA
                     APSAAEIADASAAATGPKPDELRVAIIGPGSQGRMLMMNALKIPAIRFVAVCDIWPYN
                     QLYSSNILKKYDQPCNVYTDYQEMLAQEKDLDAVIVATPDWVHAEHAVACLKAGLHVY
                     CEKEMSRNLENAKEMVLAARETGKLLQIGHQRRSNPRYLHAMKLIEKDKVLGRITHVQ
                     GQWNRPVTEDLGWPKGKELDQATLEKYGYESMHQFANWRWFKKYSGGPISDLGSHQVD
                     IFNWFLKTTPSSVQATGGLDFYTNREWYDNVLTLYDYKSADGPVRGFYQVMNTCSYGG
                     FYELFLGTEGALMISEDERRGFMFREVAAKKREWEDEAEKVENMGQEAIALKIGETLK
                     ADGTKSAEAEALMAEAQKPTLQLHLENFFNAIRNGTPLTSPAEIGYETAVCVLRVNEA
                     LEKGTRVDFKPDDFKV"
     gene            1863..>2808
                     /locus_tag="PKH07_08095"
     CDS             1863..>2808
                     /locus_tag="PKH07_08095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34942.1"
                     /translation="MMNRFVRQFLTVPIGQFWCGVLLCCGVSALVSGLSASYTYAQSK
                     DTAMRTDSLAPYVHRITLYDENGEVIDPEDDFAPVYSPKGTCGKCHAYDQIASGWHFN
                     ASDSDRPTGRRAQAWLYTDQRMGIQIPIAERDWPGVYKPEDIGLSAWQFVLTFGRHHT
                     GGGIAEPPADAEGELNARWAISGGLAIDCMICHSADGNYDQNERVGQIERQNLRWAPL
                     AASGIAAIRGAAKTLRDDYDPMMAAMTPDQAGPKVLFDKTRFDLDDRVFFDMAKRPKS
                     ERCLCCQTNRDVGEGVPESWQHDQDVHLNAGMSCADCHR"
BASE COUNT          678 a          687 c          841 g          602 t
ORIGIN      
        1 ctgtcggtga gggcgcccat cgaattgcct atcttcgaac actggtctgg tcgccagagc
       61 agaagaaggc tcaaatcgag atcggaagtg atgatggagt caaagtctgg ctcaatgatg
      121 aggtggttca cagcaagaat gtggggcgag gatgcactcc tggtgaggac aaagcccgcg
      181 ttgtgctgaa agagggctgg aaccgacttc tggtcaaagt gagcaactac acgggggggt
      241 ggggacttgc cttgagaatc cgaagtccca agggcagaac cttggacggt cttgaggttg
      301 cgttggagga atcaccgaag tagccttcag gttactgtct gctgctcacg agttgtcaga
      361 actccactga gcagcacaaa agacgtatca tgttaatcgt cacagtatct gtgacggaaa
      421 tgtttgtatg aggaggcagt tttccaaaat gccggacaag agaaagaaca cgcaagagaa
      481 gacggaactg agccgacggg atttcttcca gtcggcagcg gcgactggag taggtttgtt
      541 ttttgcgggc aaagcctttg cgcagaacga gtcatctgcc gcgcccagtg ccgcggagat
      601 cgctgatgcc tctgcggcag ccacaggccc gaagcccgat gaactcagag tggcgattat
      661 tgggccgggc agtcagggca gaatgctgat gatgaatgcg ctgaagatac ctgcaattcg
      721 cttcgtcgcg gtctgcgata tctggcccta caatcagctg tactcctcaa acatcctgaa
      781 gaagtatgac cagccctgca acgtttacac cgactatcag gaaatgctgg cgcaggaaaa
      841 ggacttggac gcggtcatcg tcgcaacgcc ggactgggta catgccgaac atgcagtggc
      901 ttgtctgaag gcggggcttc acgtctattg tgaaaaggag atgtccagga atcttgaaaa
      961 tgccaaggaa atggtgcttg ccgccagaga gaccggcaaa ctccttcaga tcgggcatca
     1021 acgtcgcagc aatccgagat acctgcatgc catgaagctg attgagaaag acaaggttct
     1081 cggtcgaatc actcatgtgc aaggtcagtg gaaccgtcca gtcacagaag atctcggctg
     1141 gccgaagggg aaggaactgg atcaggcgac gctggagaaa tacggctatg agtccatgca
     1201 tcagttcgcc aactggcgat ggttcaagaa gtattctggt ggccccattt cagatctcgg
     1261 ttcgcaccag gtggacattt ttaactggtt tctcaagacg acaccatcct cggtgcaggc
     1321 aacgggcggt ctcgactttt acaccaaccg tgaatggtat gacaatgttt tgacgctcta
     1381 tgattacaaa tccgccgatg ggccggtgcg tggtttctat caggtcatga acacctgtag
     1441 ttacggaggc ttctacgagc tttttctcgg caccgaaggg gccttgatga tctccgaaga
     1501 cgaacgcaga ggcttcatgt tccgtgaagt ggcggcaaag aaacgcgaat gggaagacga
     1561 agcggagaaa gtcgagaata tggggcagga agccattgcc ctcaagatag gagaaacgct
     1621 caaggctgat ggcaccaagt ccgctgaagc agaggccttg atggcagaag cgcagaagcc
     1681 gacgctgcag ctgcatctgg agaacttctt caacgccatc cgcaacggga cacccttgac
     1741 gtctcctgct gaaatcggat atgagacggc tgtgtgcgtt cttcgggtca acgaagccct
     1801 tgaaaaaggt acgcgcgttg acttcaagcc ggatgacttc aaagtctagg atcggacaag
     1861 tcatgatgaa ccgctttgtg cgtcagtttt tgacggtgcc aatcggccag ttttggtgtg
     1921 gggtcctttt gtgctgtggt gtctctgcgt tggtgtccgg gctttctgcc tcctacacgt
     1981 acgctcagtc gaaagacaca gccatgagga cagacagcct tgcgccgtac gtgcatcgca
     2041 taacactcta cgacgagaat ggtgaagtca ttgacccaga ggatgacttt gcccctgtct
     2101 actcacccaa ggggacttgt gggaagtgtc acgcctatga tcagatcgca tcgggttggc
     2161 atttcaatgc ttccgactcc gaccgcccta cgggcagaag ggcacaggcc tggctctata
     2221 ctgatcagcg aatgggaatt cagattccaa tcgcggaacg ggattggcct ggtgtttaca
     2281 agcccgagga tatcggcctt agcgcatggc agttcgtact cacctttggc aggcaccaca
     2341 cggggggagg cattgctgaa ccgcctgccg acgccgaagg agaactgaac gcgcgatggg
     2401 cgatttcggg tggtcttgcg attgactgta tgatctgtca cagcgcggat gggaactacg
     2461 atcagaatga acgtgtggga cagattgaac ggcagaacct ccggtgggca cctttggctg
     2521 caagtgggat tgccgccatt cgaggcgcgg caaagacgct tcgtgatgac tatgatccca
     2581 tgatggccgc aatgactccg gatcaggcgg gtcccaaggt cttgttcgac aagactcgat
     2641 ttgacctgga cgatcgtgtc ttcttcgaca tggccaagag gccgaagtcg gagcgatgcc
     2701 tttgctgtca gacaaatcga gatgtcggag aaggagttcc ggaatcctgg cagcacgatc
     2761 aggacgtgca tctcaatgcg ggaatgtcct gtgccgattg ccatcggc
//
LOCUS       DAOQUC010000414         2806 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_42421_length_2806_cov_3.181025, whole genome shotgun sequence.
ACCESSION   DAOQUC010000414 DAOQUC010000000
VERSION     DAOQUC010000414.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2806)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2806)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2806
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42421_length_2806_cov_3.181025"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1456)
                     /gene="uvrB"
                     /locus_tag="PKH07_08100"
     CDS             complement(<1..1456)
                     /gene="uvrB"
                     /locus_tag="PKH07_08100"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HNT34943.1"
                     /translation="MRSFKLVSDYDPSGDQPQAIERLTKGLRSDMKYQTLLGVTGSGK
                     TFTIANVIANVNRPTLVISHNKVLAAQLYSEFREFFPENAVEYFVSYYDYYQPEAYLP
                     KTDTYIEKEVTINEEIDRLRLAATTALLQRRDVIIVASVSCIYGLGTPEDFKQMILML
                     EVGQTIRREDILRQLVALQYSRNDVDFYRGMFRVRGDIIEIFPAYEEVPYRICLFGDE
                     VEQLGEFEPLRGNMVRPMDQLVIYPAKHFVMPKERLQGAFQAIRDELRERLIELRSQG
                     KLLEAERLEQRTTFDLEMLSEIGYCQGIENYSRHLSGRAPGEPPACLVNYFPSDFLLI
                     VDESHIMLPQLRGMYAGDRSRKMTLVEYGFRLPSAMDNRPQQFDEFFRLVSQAIFVSA
                     TPADYELGISDQVVEQIIRPTGLVDPVVVVVPATGQVDDLYGRIRERVDRGERVLVTT
                     LTKRMAEDLAKYFEEMGLKVRYLHFEVETLERVEI"
     gene            2088..>2806
                     /locus_tag="PKH07_08105"
     CDS             2088..>2806
                     /locus_tag="PKH07_08105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34944.1"
                     /translation="MNRRMGFVCVVWGWIMVLLVWACPVWAQETLETFTITKIEPDPE
                     QESVNITFSKPVDWTTLERKIQFVPWARLYWYSSEYQSKTNSLFLKGSFKNGVQYRLS
                     FPQGTKNGKGMVYQETVCTFFMPDKPPKLALAEKQTLIERDSRQMVHLNLTNVDELLF
                     EGVEIPPALIPLVRSASDGISGQKLAPVVERLRQWNQPIADALSRHAQLGIPAGEVSQ
                     AMQLFFTQAEKNKDIRFSAPLT"
BASE COUNT          637 a          736 c          751 g          682 t
ORIGIN      
        1 taatctcgac tcgctccaga gtctccactt caaagtgcag ataccgaacc ttgagaccca
       61 tctcctcgaa gtatttcgcc aggtcttctg ccatgcgctt ggtgagtgtc gtaaccagaa
      121 cacgttcgcc acggtcaacg cgctcgcgga tgcgtccata gagatcgtcc acttgacccg
      181 tggccggcac cacgacgacc actggatcca ccaaccctgt cggccggatg atctgctcga
      241 cgacctggtc gctgataccc agttcgtaat cggcaggcgt cgcggatacg aagattgcct
      301 gcgagacaag acggaagaac tcatcgaact gctgcggtcg gttgtccatc gcggacggca
      361 gccggaatcc gtactcgact agagtcatct ttcgagatcg gtcgccagca tacatccccc
      421 gcaactgcgg cagcatgata tgcgactcat ccacgatcag caggaaatct gacggaaagt
      481 aattcacgag acaggctggt ggctcgcctg gtgccctgcc tgaaagatgt ctcgaatagt
      541 tttcgatgcc ctggcaatac ccgatctcgg agagcatctc cagatcgaag gtcgtccttt
      601 gctccagtcg ttcggcctca aggagtttcc cctgcgaacg cagttcaatc agccgttcac
      661 gcagctcatc ccgaatggcc tggaacgcgc cttgaagtcg ctccttgggc atgacaaagt
      721 gcttggccgg atagatcaca agttgatcca tcgggcgaac catgtttcct cgcaacggtt
      781 caaattctcc cagttgctcc acttcgtccc caaagagaca gattcggtag ggaacctctt
      841 cataggcagg gaaaatctca atgatgtctc cacgtacgcg aaacataccc cgatagaaat
      901 ccacatcgtt gcgcgagtac tgcagcgcca ccagctggcg cagtatatct tctcgacgga
      961 tcgtctgccc gacttccaac atgagaatca tctgcttgaa atcttccggt gttcccaaac
     1021 catagataca agacacagag gcaacgatga taacatcgcg gcgttggagc agggcagtgg
     1081 tcgccgccag tcgaagccgg tctatctctt cattgatggt gacctctttc tcgatgtagg
     1141 tgtccgtctt gggcagatag gcttccggct ggtagtaatc atagtaggac acaaagtact
     1201 cgacagcatt ctcgggaaag aactctcgaa actccgaata cagttgagcc gcgagaactt
     1261 tgttgtggga gatgaccagc gtcggacggt tcacgttggc gatgacgttg gctatcgtga
     1321 acgttttgcc agagcccgtg accccaagca gggtctgata cttcatgtcg gacctgagac
     1381 ccttcgtaag gcgttcgatg gcttgcggtt gatctcccga cggatcataa tctgagacaa
     1441 gtttgaatga tctcactcga caacatcctc gacagtcgca actgacgtca atttggtata
     1501 ctagccttgg cgtttgtctt tcggcaagtc gtgacagagg gaggtctcgg atgaagacgt
     1561 tattcccagg agtgtttccc atgttccttc ttctgtcttg tttggccagt agacccacgg
     1621 tcgttggcac ggaacaagtc gaaccgttct gtaacatctg tgtaagtgtc cttgagacga
     1681 tgggttccat caaacacaac gcgttgggct ttctccactc gtttcgtgct ggaacagacc
     1741 attgaaagcc ctgcgaaaga gatgcgaatt catctgccga gtctggagcc tgctgcagcg
     1801 atttcggttg tgctcagtgg gatatgatct gccggtgacg gacttgtctg ccacgccgct
     1861 tgccctcggt ctgcaggagc gtagaattat tttcagaatt ggtgacagcg ctcgaataag
     1921 ttcttgtggc aggcgtcaat gagggtgtca gatcgcgatg tcacttccag tacactgctt
     1981 cttgctgtga gtgcagacag acggcatgtc tggggtgata ggaatgttgg agctgttgct
     2041 ggggtatgcc ctgatagtgg gcgggttttt ccgaaaggag caggacgatg aatcggagaa
     2101 tggggttcgt gtgtgttgtt tggggttgga tcatggtttt gttggtctgg gcgtgtccag
     2161 tctgggcaca agagactttg gagaccttca cgatcaccaa gatcgaacct gatcccgaac
     2221 aagagagcgt caatatcacc ttcagcaagc cggtggactg gactaccctg gaacgcaaga
     2281 tacagtttgt tccctgggcg agactttact ggtattcgag cgaataccag tcaaaaacca
     2341 attccctttt cctcaagggg tctttcaaga acggtgtcca gtaccgactt tccttcccgc
     2401 aaggaaccaa gaatggcaag ggtatggtct accaggagac cgtttgcacc tttttcatgc
     2461 cagacaaacc gcccaaactg gcgttagcgg aaaagcaaac cctgatcgag cgagatagtc
     2521 gccagatggt tcatctcaat ctcaccaatg tagatgaatt gctttttgag ggtgtggaga
     2581 tcccccctgc tttgattcct ctggtgcgtt cggcatccga tgggatttct ggtcagaaat
     2641 tggcacccgt tgttgaacgc ctgcgccaat ggaatcagcc cattgcggat gcgttgtcaa
     2701 ggcatgctca gttgggcatc cctgcggggg aagtctctca agccatgcag ttgtttttca
     2761 cacaagcgga aaagaacaag gatatccgat tttctgctcc cttgac
//
LOCUS       DAOQUC010000415         2803 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_42485_length_2803_cov_3.382460, whole genome shotgun sequence.
ACCESSION   DAOQUC010000415 DAOQUC010000000
VERSION     DAOQUC010000415.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2803)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2803)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2803
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42485_length_2803_cov_3.382460"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2803)
                     /locus_tag="PKH07_08110"
     CDS             complement(<1..>2803)
                     /locus_tag="PKH07_08110"
                     /inference="COORDINATES: protein motif:HMM:NF021570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2298 domain-containing protein"
                     /protein_id="HNT34945.1"
                     /translation="GMLAAFFIGLMGNFQSFLRLLHNLTTVASTVQGHPFQSVGAQLE
                     RLNAGQGLLSRLLYFVAGLWASLKYVLSLVWSAGWNQTDGHGHIPLVGFDSYYWKCGH
                     DIIPQTAANEFPLWSFLFADLHAHIIVMPFFLLFITLCLNLLLAVGCSRPSATSGRRL
                     LAEGEPETSSNATDRAEHYASVPSFLFPWRFLVYALSFGVVGCINAWDLPSSVLILLM
                     TLGLLLFNYRLECSVGALLRGVHWMKKMRIARAWQRRLLMAAHRTLNQIVVPFLLILV
                     TGYLLFLPFHIHFNPVIKMSLESVNLLRYGMTNPLAFLKIFAFFIFILVGYLVLRLYR
                     ISRWSMWALIPCLLFLLLPQGLVGVTKLIERFGASPWQVLTLCSQYHLAASLVPVVLL
                     ALTLMLRRTNSKEASFGLMLCLLGLGIACGLEIWYIQEFMGARFNTLFKLYLEVWNLW
                     SLVAAYFLYRTFDPSWTWPLLWARVFRRTVKVVWGVSFAFLLLAGCLFCVAGPYSLIA
                     TNAHCNRASIATMNGLQFKLEGKPTEYWAILWMNRFLRNSPTIAESHAERIYSDYSRI
                     SMYTGLPVIIGWDNHIKERGHAREIETRTRDLRQIYLEKDPKRFSTLLDKWKVQYLYF
                     GDLERDYGLSSLERLKTFGHRLDLVYQDGDVNIFQARPGWSADQMQYPWTESLGLDQQ
                     EPGVNLFQSGRGDGNGNFVGPRGIAVDAQGDIYVADTFNHRIQKFGSNGDFKRTWGEM
                     GAMEEQFKEPNDVLPDGTRILVADTWNHRIQVFDLNGRPLTDWDTKNAIPLFGPRALT
                     SDGSGHFFVSDTGNARIAILNSRGTITKVFGERGTEQRQMLEPVGLAVSPDKRLFVAD
                     TRNDRVQIFTLEGDFLKSFPTRTARSGDDSNEAHMAISKSGRLFLTDPAGLGVVEYDL
                     DGTFIRQYQADSQGRALVSV"
BASE COUNT          710 a          776 c          756 g          561 t
ORIGIN      
        1 caacggagac aagtgcacgt ccttgtgaat cggcctggta ttgtcggatg aaggttccat
       61 cgagatcata ttccacaacg cccaagcctg ccggatcggt gagaaacaga cgcccgctct
      121 tgctgatggc catgtgcgcc tcgttggagt catctcccga acgggcggtg cgagttggga
      181 acgacttcag gaagtctcct tcgagtgtga atatctgtac gcgatcgttt ctcgtgtccg
      241 cgacgaacaa acgcttgtcg ggagagacgg caaggccaac cggttcgagc atctgcctct
      301 gctccgtgcc ccgctcccca aagaccttgg tgatcgtgcc tcgtgagttg aggatggcga
      361 ttcgagcgtt tcccgtatcg gacacaaaga agtgacccga accgtccgag gtcaacgccc
      421 gcgggccaaa cagcggaatg gcgttcttgg tgtcccagtc ggtgagtggt cgtccgttga
      481 ggtcgaaaac ctgaatccga tgattccatg tgtccgccac aagaattcga gtgccatcag
      541 gcagaacatc gttgggttcc ttgaactgct cttccatagc acccatctcg ccccaggtac
      601 gcttgaagtc accgtttgag ccgaacttct ggatccgatg gttgaacgtg tcggccacgt
      661 agatgtcccc ttgggcgtcc actgcgatgc cccgcggtcc gacgaaattc ccgttcccat
      721 cgcctcggcc tgactgaaag aggttgactc ccggttcctg ctgatccagg ccaagactct
      781 ccgtccaggg gtactgcatc tgatcggcgc tccaacccgg tcgtgcctga aagatgttca
      841 catcaccgtc ctgatagacc agatccagtc tgtgtccaaa tgtcttcagc cgctccagac
      901 tcgacaggcc atagtccctt tccagatcgc cgaagtagag gtactgcact ttccacttgt
      961 ccaggagagt ggagaaacgt ttgggatcct tttcgagata gatctgcctc aaatcgcgtg
     1021 ttcgagtttc gatctctctc gcgtgtcccc gttccttgat gtggttgtcc cacccaatga
     1081 tgactggaag tcccgtgtac attgagatgc gtgagtaatc cgaatagatg cgctctgcgt
     1141 gactttccgc aatcgtcggg gaattcctca gaaagcgatt catccagaga atcgcccagt
     1201 actccgttgg cttgccttcg agcttgaact gcagcccgtt catggtggca atggaggctc
     1261 tgttgcagtg ggcattggtg gcgatcagag agtaaggacc cgcaacgcaa aacagacatc
     1321 ctgccagcag cagaaaggcg aaactcactc cccagacaac tttgaccgtt cggcgaaaga
     1381 cacgtgccca aagcagtggc catgtccagg agggatcgaa ggttcggtac aagaagtatg
     1441 cggccaccag tgaccacaga ttccaaactt ccagatacag cttgaacaac gtgttgaaac
     1501 gcgcccccat gaattcctga atgtaccaaa tctccaaccc gcaggcgatg ccaagtccca
     1561 acaggcacag catcaaccca aaagaggctt ccttggaatt ggttcttcgg agcatcaagg
     1621 tcaaggccag cagtaccacg ggcaccagag atgccgccag atgatactga gagcagagag
     1681 tgagaacttg ccagggactt gcgccaaaac gctctatcag cttcgtgacg ccgaccagac
     1741 cttgcggcaa cagcaaaaac agcaggcagg gaatcaaagc ccacatgctc catcggctga
     1801 ttcgatacag acgcagcacc aggtatccga ccaggatgaa gatgaagaac gcaaatatct
     1861 tcagaaatgc caagggatta gtcattccgt agcgcaagag attgacactc tcaagactca
     1921 tcttgatgac cggattgaaa tggatgtgaa acggaagaaa gagcagataa cccgtgacca
     1981 gaatcagcaa gaaaggaacg acaatctgat tgagcgttcg gtgagccgcc atcaagagcc
     2041 ggcgttgcca ggctcgggca atgcgcatct tcttcatcca gtgtacaccc cggagcagcg
     2101 cacccacgct gcattcgagc cgataattga acagaagcaa tcccagtgtc atcagcagga
     2161 tcagaacgct tgaggggaga tcccatgcgt tgatgcaccc gacgacacca aacgacaacg
     2221 catagacgag aaatctccac gggaacagaa aagaaggaac gctggcgtaa tgctcggcac
     2281 gatccgttgc gttggaagaa gtctccggtt ctccttccgc caggagccgc cttcccgatg
     2341 tggcacttgg tcgggaacac ccgactgcca gaagcagatt caggcacagt gtgatgaaga
     2401 gcaggaaaaa gggcatgacg atgatatgcg catgaagatc ggcaaagaga aatgaccaca
     2461 gtgggaactc attggcggcg gtctgcggga tgatgtcgtg accgcatttc cagtaataac
     2521 tatcgaaacc gacaagagga atgtgcccgt ggccatcggt ctggttccag cccgcgctcc
     2581 aaacgagaga gagcacgtac ttgagcgatg cccaaaggcc ggcgacgaaa tagagcagcc
     2641 gtgacaacaa tccttgaccg gcattgagtc tttccaactg agcaccgaca ctttgaaatg
     2701 gatgcccctg cacggttgat gcgacggttg tcagattgtg caacagtcgc agaaaactct
     2761 gaaagtttcc catcaggccg atgaagaatg cggcaagcat ccc
//
LOCUS       DAOQUC010000416         2801 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_42549_length_2801_cov_1.942462, whole genome shotgun sequence.
ACCESSION   DAOQUC010000416 DAOQUC010000000
VERSION     DAOQUC010000416.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2801)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2801)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2801
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42549_length_2801_cov_1.942462"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..912
                     /locus_tag="PKH07_08115"
     CDS             <1..912
                     /locus_tag="PKH07_08115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA+ family ATPase"
                     /protein_id="HNT34946.1"
                     /translation="GPAPDVGGAVLQVRQAVAQKTDADSAVTARLPEAYQWLLSPTQS
                     SPQAPLQWQAIRLSGQESLAERASKKLRNEELLVTVFASSRLRMELDRVPLWRGEHVK
                     ISQIIEDFARYNYLPRLKNSTVVMQAIADGIGLLTWMMDSFAYADGYDQKAERYQGLR
                     GGERISVSEEGLLVKPDIAQRQIEKETRKPEKQEELTNPPSETKLKEPSGKTPETEQT
                     PVPPPAPKPKRFHGSVILDTERVGRDASKVAEEVIAHLSVLPESKVKVTLEIEAILPE
                     GAPENVVRTVTENCQTLRFETQGFEKD"
     gene            complement(1425..1763)
                     /locus_tag="PKH07_08120"
     CDS             complement(1425..1763)
                     /locus_tag="PKH07_08120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34947.1"
                     /translation="MRRAERKRETREQAEREKKEAEARQKYLDSLRGKENEIWEQIEK
                     LILTRQPRRYDEAPALPRDLRDSATMQDRKGDYDVRLDALKQRHSQKSALLRRLKHTV
                     VTGIAADGLG"
     gene            complement(1841..2110)
                     /locus_tag="PKH07_08125"
     CDS             complement(1841..2110)
                     /locus_tag="PKH07_08125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34948.1"
                     /translation="MALTLEARSTLGKLPTCATGYTATHPSLFPVRPVIAQYPSSRKS
                     DIASARIAGVDLEVSWNFKHIVHFEKRAGYQGVNLIHGYPAIRAP"
     gene            2413..>2801
                     /locus_tag="PKH07_08130"
     CDS             2413..>2801
                     /locus_tag="PKH07_08130"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNT34949.1"
                     /translation="MNQDRKHILIADDEERILSSLSMALENIGYDVSTASSGSEAMEK
                     LLGCIEGSRPVDLLISDIQMAGMNGLELLDKINDSGLEIPVMVITGFGDKGMVVELMR
                     KGCADYIDKPLEPVSFTNRVQAVLNSSS"
BASE COUNT          703 a          701 c          735 g          662 t
ORIGIN      
        1 gggccagcac cagatgtggg cggggcagtt ctacaagtgc gacaagccgt ggcacagaag
       61 acagatgcgg acagcgcggt caccgcacgc ctgccggaag cctatcaatg gctgctttca
      121 cccacacaga gttctcccca ggctccatta caatggcagg ctattcgact ttccggacag
      181 gaatcccttg ccgaacgtgc cagtaagaaa cttcgcaatg aagaactgct cgtgacagtt
      241 ttcgcctctt ctcggctgcg aatggaactg gaccgtgttc ctctctggcg tggtgaacat
      301 gtcaagatca gtcagattat agaggatttc gcccgataca actatctgcc cagactcaaa
      361 aactccactg tggtcatgca ggcaatcgca gatggcatcg gattgttgac ctggatgatg
      421 gactccttcg cctacgcgga cggctatgat cagaaggcag aacggtatca gggtctgcga
      481 ggtggggaaa gaatctccgt cagcgaagag gggctgcttg ttaagccgga tattgctcag
      541 agacaaatcg agaaagagac caggaagccc gaaaagcagg aagaactaac caatcccccc
      601 tctgaaacga agctgaaaga gccctcagga aagacgcccg aaacggaaca aactccggtt
      661 ccgccaccag caccaaaacc gaaacgcttc cacgggtcag tcattctcga cacggagcgt
      721 gtcggcagag acgcttccaa ggtcgccgag gaagtcattg ctcatctctc cgtccttccg
      781 gaatccaaag ttaaggtgac tctggaaatc gaagcaatcc ttcccgaagg cgctcctgaa
      841 aacgtggttc gcaccgtgac cgagaactgt caaacgctga gattcgaaac acaggggttt
      901 gaaaaggact gagagacttt tccgcaggca acaggagaaa agatggagcg ggaaacgggg
      961 ttcgaacccg cggccttcag attgggaaag tgttcttcga ttttcgagtc cagcccgatt
     1021 tgaccgtaac tggtagggtt tagaaggatt tactgcaact acattctcgg ctttgacttg
     1081 gttttgccgt gtctgacctg atccatttgc acaccagatt gcccaccaaa tcctcttggg
     1141 cgaaagcctt tcgcggaatg gcggccatgt gttattcttc tcttggaggc ggatctagag
     1201 atgaaaccag agaagagatt tgactgcgtg cggatgaaga atgaaatcca ggcaaagatt
     1261 ctgaagcgtt tggagggcat gtcgcctcac gaagcgagat tggccaagag gcgaatggtg
     1321 gaagaggacc caatactcgc aaaattcgca aagcacacga gaccttcctc acagatcact
     1381 cgaaacagac ttgatcatat ctagcgccgc cgaaggccaa ttcttcatcc aagtccatcc
     1441 gcggcaatcc ctgtgacgac agtgtgtttg agtcgtctaa gaagcgcact cttctgcgag
     1501 tgtctctgtt tcaacgcgtc aaggcgaaca tcatagtcgc cttttctatc ctgcatagtc
     1561 gccgagtctc gtaaatcccg cggcagagcc ggcgcctcgt catagcgcct tggctggcga
     1621 gtcaggatga gtttttcgat ctgctcccag atttcattct ctttacctct tagcgaatcg
     1681 aggtatttct gcctcgcctc ggcttccttc ttttcgcgct ctgcttgctc gcgagtttcc
     1741 cgctttcttt cggcgcgtct catgaatctg aaggggagta gcgctacagc gtgggggatg
     1801 tcaatgtgtt tggaatcgct aatcttgcgt cattctggat tcatggcgcc cgaatcgctg
     1861 gatagccatg gatcaggttc acgccttgat agcctgctct cttctcaaag tgaacaatat
     1921 gcttgaaatt ccagctgacc tcgaggtcaa ccccagcgat ccttgctgaa gcaatatcag
     1981 actttctcga agaaggatac tgagcaatta ccggtctcac gggaaaaagg ctgggatgtg
     2041 ttgcggtgta gccggtggca caggtgggaa gtttgccaag tgtagacctc gcctcaagag
     2101 tcaatgccac aaccgaatca catggattct catgagcagt tcgggtcagg gaattctatt
     2161 gtcgtatcgg gtgtcaagtt catgtgttgc catgtcacca cgaaatgcaa cttatctcat
     2221 taagaatcag actgctattg atagtttcgc acattgaccg taaaatgtct taacacggtc
     2281 tattggattg ttctcagagg tatcctcttt cataagcact tctttttgag caacttactc
     2341 catcattctc aagttggcac gctgtctgct aatacttggc tgcagaaatg cgatgaacga
     2401 ggaggcgtat ccatgaatca agacaggaag cacatcctga ttgcggatga tgaagagaga
     2461 atcctcagtt ccctttccat ggcactggag aatattggtt acgatgtgtc cacagcaagt
     2521 tcgggctccg aggcgatgga aaaactgctc ggctgtattg agggaagcag accggttgac
     2581 ctgctgatat ccgacattca gatggcgggc atgaacggac ttgaactact ggataagatc
     2641 aatgacagcg gcctggagat accggtgatg gtgatcacag gctttggtga taaaggaatg
     2701 gttgtcgaac tgatgcgcaa gggttgtgct gactacattg acaagccttt ggaacctgtc
     2761 tctttcacca acagagtgca agcagtcctg aactcatcgt c
//
LOCUS       DAOQUC010000417         2799 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_42587_length_2799_cov_2.551385, whole genome shotgun sequence.
ACCESSION   DAOQUC010000417 DAOQUC010000000
VERSION     DAOQUC010000417.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2799
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42587_length_2799_cov_2.551385"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1120)
                     /locus_tag="PKH07_08135"
     CDS             complement(<1..1120)
                     /locus_tag="PKH07_08135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34950.1"
                     /translation="MNRREAMQTILTGAGAMTCIAATETSAEQSKHSASAKPIEKDAL
                     CFWLETSLRRVFPNSSPRDASPLHLLAARNEQISFQACFRNNRTTFVRVRLSVQGAPE
                     CSVSIRRVGYVPLQGLNTDTPKSDLDGIGHIPGLVPDPLFPEDSASVGPESNGAFWIT
                     IKTPADLSPGLRTLTVRFTFEDEFSFPVWLGTAPFSVEIPVFVEVCPLVIESRKDFSA
                     THWINIESIWDYYKTAPFSERFWELAETYIANMVTHNLDVVYTPIFNARHEIMPRPGQ
                     LLRVRRLDEDQYDFDFTDVRRWVQLALKHGAQKLEFTHFFTPAPTSGKHPQHIFHRWP
                     QTRDILWSPDISATSNVYRNFLGQFLPQFGRFLRDEGIL"
     gene            complement(1158..1904)
                     /locus_tag="PKH07_08140"
     CDS             complement(1158..1904)
                     /locus_tag="PKH07_08140"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HNT34951.1"
                     /translation="MDHLSENVWTWCKSGQFVCAFALLLCFSGCSLKQQAIEVLEREY
                     NEARWEETIRKFEEKDKVQPPKPNGILFIGSSSVVRWDTEKYFPGYGVINRGFGGSQI
                     VDSVHYADRIVIPYKPALIVFYAGNNDIKAGKPPEEVAADFEKFRAVVHKELPETRII
                     YISIHPSVSRWSHDPEMRKANSLIKKQCSKDKRLTFVSIYKEMLGKDGTPNKELLDPD
                     MLHLSPKGYEVWTKKLTPYLDKFMKTKKSR"
     gene            2057..2377
                     /locus_tag="PKH07_08145"
     CDS             2057..2377
                     /locus_tag="PKH07_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNT34952.1"
                     /translation="MEEGIQARYEARARVLKALSHPTRLFIVDQLSLRDHCVNELTEM
                     IGSDVSTVSKHLSVLRNAGIIDDDKRGTQVFYSLRVPCITQFFGCVEAVVKTQAEHQI
                     ASVR"
     gene            2464..2697
                     /locus_tag="PKH07_08150"
     CDS             2464..2697
                     /locus_tag="PKH07_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNT34953.1"
                     /translation="MKKIQILGTGCSKCKKLTGNAEQAVRALGADCAVEKVTDINEIL
                     KFGVMMTPALAIDGEVKSVGKVLSADDIKVLIQ"
BASE COUNT          676 a          669 c          760 g          694 t
ORIGIN      
        1 gcagaatccc ctcgtccctg agaaaccgtc cgaactgggg taggaactga ccgagaaagt
       61 tgcgatagac attggatgtg gcgctgatgt ctggactcca gaggatatcc cgcgtctgag
      121 gccagcggtg gaagatgtgc tgagggtgtt tgccactcgt cggtgccgga gtaaagaaat
      181 gggtgaactc gagcttctga gcgccatgct tcaaagccaa ttgcacccat cgtcgaacat
      241 ccgtgaagtc aaaatcatat tgatcctcat caagacgtcg cactcgaagc agctgcccgg
      301 gtctaggcat gatctcatgt cgggcgttga aaatgggtgt atagaccacg tccagattgt
      361 gcgtgaccat gttggcgatg tacgtctctg ccaactccca gaaccgctcg ctgaaaggtg
      421 cagtcttgta gtagtcccag atggactcta tgttgatcca gtgtgttgcc gagaaatctt
      481 tacgagactc aatcacaaga ggacagactt caacaaagac agggatttca acagagaagg
      541 gagccgttcc cagccagaca gggaaactga actcatcctc gaacgtaaaa cgaacggtga
      601 gcgtgcgcag tccgggagac agatcagcag gagtcttgat ggtgatccag aacgcgccgt
      661 ttgattctgg tcccacgctt gcggaatctt cgggaaagag agggtcggga acaaggccag
      721 gaatgtgtcc aataccatcc agatcgctct tgggagtatc tgtattcaag ccctgcaggg
      781 gaacatagcc cacccttctg atggagaccg agcactcggg agcgccctgc acactcagtc
      841 ttacccgaac aaatgtcgtg cggttgttgc ggaagcacgc ctgaaaagag atctgctcgt
      901 ttcgagctgc caagaggtgg agcggggagg catctcgagg ggacgagttt ggaaagactc
      961 ttctcagtga ggtttcaagc cagaagcaaa gcgcatcctt ttcgatgggc ttggcagagg
     1021 cagaatgctt ggattgttct gctgaggttt ccgtcgctgc aatgcacgtc atggctcctg
     1081 ctcccgtaag aatcgtctgc atcgcctctc gtcggttcat atcgtgctct ccagttcaga
     1141 aaaactaatg tcgtaatcta tcgggacttc ttcgtcttca tgaacttgtc gagatagggt
     1201 gtgagtttct ttgtccaaac ctcatagccc ttcggcgaaa gatgcaacat atccggatca
     1261 agcagttcct tgttcggagt tccgtccttg cccagcatct ctttgtagat gctcacaaat
     1321 gtcagcctct tatctttgga acactgcttc ttgatcagag agttggcctt gcgcatctca
     1381 ggatcatgac tccatcttga gacgcttgga tgaatgctga tgtagatgat cctggtctca
     1441 gggagttcct tatgaacaac agcacggaat ttctcgaaat ccgccgcaac ttcctccggt
     1501 ggtttccccg ctttgatgtc attgtttcca gcgtagaaga ctatcaaagc aggtttgtat
     1561 gggataacga ttcggtcggc gtagtgcacc gagtccacta tctgagagcc cccgaatccc
     1621 ctgttgatga caccgtatcc tgggaaatac ttctcagtat cccaccgcac gacactcgaa
     1681 cttccgatga acagaattcc gttgggtttg gggggctgga ctttgtcctt ctcttcaaat
     1741 ttccgaatcg tctcttccca gcgtgcttcg ttgtattcgc gctcgaggac ctcgatcgct
     1801 tgttgcttca aagaacaccc cgaaaaacac aaaagaaggg caaaagcaca cacaaactgt
     1861 cctgacttgc accatgtcca gacgttttct gacaaatgat ccatctcatg cactcctcga
     1921 tgaaagaatt gccgtgaagg ctttagggcg atcttagacc acacaagcgc aagatggtca
     1981 aggagagaaa tgggttgacg cgacttttct attttgttag tttaccaaat agccaaatag
     2041 agtataagga gtgaagatgg aagagggaat acaggcacgc tatgaggctc gggcaagagt
     2101 gcttaaggct ctgtcgcatc cgacacgatt gttcatcgtt gatcagctgt ctttgcgcga
     2161 ccattgtgtc aatgagctga ccgagatgat tggatcggat gtttcgacgg tttccaagca
     2221 tctgtcggtg ctgcgaaacg ccgggatcat tgacgatgac aagcgtggaa cacaggtatt
     2281 ctactcgctt cgggttcctt gtatcacgca gttctttggg tgcgttgagg cggttgtgaa
     2341 gactcaggcg gagcatcaga ttgcatcggt acggtgaggg gtctctgttg ccaagagctc
     2401 atctgatcgt gtgaaagagc gagtcggaga tcccgaccgg ctgctgcaga caggagtagt
     2461 tcattgaaga agatacagat tctggggacg ggctgttcca agtgtaagaa actgactgga
     2521 aatgccgagc aggctgttcg tgcacttggt gccgactgcg ctgttgagaa agtgaccgac
     2581 atcaatgaga tcctcaagtt tggcgtcatg atgacaccgg ctttagcgat tgatggtgaa
     2641 gtcaaatcgg tcggaaaagt gttgtctgct gatgacatca aggttttgat tcaatagttc
     2701 aaaggagtta catgatgaaa gacgaatgcc tctgcactgc tgctcccact ttgatattca
     2761 gttgctctgg cgcggctgac gtgggtgcta tcgctgatc
//
LOCUS       DAOQUC010000418         2797 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_42623_length_2797_cov_3.266594, whole genome shotgun sequence.
ACCESSION   DAOQUC010000418 DAOQUC010000000
VERSION     DAOQUC010000418.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2797
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42623_length_2797_cov_3.266594"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            112..2244
                     /locus_tag="PKH07_08155"
     CDS             112..2244
                     /locus_tag="PKH07_08155"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNT34954.1"
                     /translation="MVRSRKRDPKSAVAVSPHAEALREMAQRYENLVQNLSILRQLDE
                     LDRPDREMQVLCREMVETIAFGLAAENCSLMLLDHEKTCLQLRAACGPYQTKGQSYAP
                     GEWGGESFPLGYGIVGKVAQAGVPIRVNDVSKEKDFLPRQDSLVELGSLMSFPLRCEG
                     EVIGVLNVSHREKGFFSAESLATLALIAERTAQIISNRLVHEELRRSEIHLREILENA
                     GDAILVYDASGELIDANSAVERITGIAPQKYIQQEVKWESGVHPDDAEQYTVLLSSSL
                     ATGKPELFEYRYLDPQGGIHYLEQKNSRFLDSSNQVERIVVTIRDTTERRQLQEQLIK
                     AQKMESLGVLAGGVAHDFSNLLTSIVGNSSLMLSEMPSESPLQEGLKQIAKAAEIASG
                     LCRQMLAYSGKGRFVVLPINLTDVVNEMKDLLDVTVSKTVEIRYELDTSLPCVEADAA
                     QMYQVISNLVTNASESIGNRKGTVTIKTASRECTREFFADCYLNLVLQEGSYVMLEVS
                     DTGCGMTPETMQKMFDPFFTTKFTGRGLGLSAVLGIVRGHSGTIQVNSEPGQGTTFRL
                     LLPATDRCPSNHQPAHVESKTFLRGLTILVVDDDPAVLDLIELILDGEGSKVLKATDG
                     LEALDVYQAHAKEIALVILDMTMPNLSGEDTLFRLRAIDPDVRVLLSSGYSETPEKFR
                     FEEWGAKGFIHKPYPVADLIKAIERSLD"
     gene            complement(2354..2554)
                     /gene="rpmE"
                     /locus_tag="PKH07_08160"
     CDS             complement(2354..2554)
                     /gene="rpmE"
                     /locus_tag="PKH07_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013626279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HNT34955.1"
                     /translation="MKKDIHPEYHQTKIVCGCGNVIETGSTKQNIRVEICSNCHPFFS
                     GKQRFVDTAGRIDRFKKRYKMK"
BASE COUNT          643 a          690 c          791 g          673 t
ORIGIN      
        1 tttcgtattt tggccttgaa cagacatctc tggaaagcct tgccgaagac ttacagacct
       61 tccgtcagga gacatgtcag tttctcgagt tactcaaggg gggtggaaat aatggtacgt
      121 agcagaaaga gagatccaaa atctgcggtt gctgtctccc cgcacgctga agcgttgcgt
      181 gagatggcgc aacgatacga aaaccttgtt cagaacctat ccattctgag gcagctggat
      241 gagctggatc gaccggaccg ggaaatgcag gtgctttgtc gagagatggt cgagaccatt
      301 gcctttggtc ttgctgccga gaattgctcc ctgatgctct tggatcacga gaagacttgt
      361 cttcagttga gagcggcctg tgggccctat cagaccaagg gacagagtta cgcccccgga
      421 gagtggggtg gagagtcctt tcccttgggg tatggcattg tgggaaaggt tgcccaggca
      481 ggagttccga tccgggtcaa tgacgtttcc aaggagaagg attttcttcc aaggcaggac
      541 agcctcgtgg agcttggctc gctcatgagt tttcccctgc gctgtgaggg ggaggtcata
      601 ggagtcttga atgtcagtca tcgggagaaa ggattcttct ccgccgagag tctagccacg
      661 ctggctctta tcgcggaacg aacggcacag atcatctcca accggttggt tcacgaagaa
      721 ctgaggcgat cagagatcca tctgcgtgag attcttgaga acgcgggaga cgccatcctt
      781 gtctatgatg ccagcgggga gttgatcgac gccaactctg cggtcgagcg catcaccggc
      841 atcgcgcctc agaagtacat tcagcaggaa gtcaagtggg agtcgggagt ccatcccgat
      901 gatgcagaac agtataccgt gctgttgtcg agttcccttg ccacaggcaa gcctgagcta
      961 tttgagtatc gttatcttga cccccagggg ggtattcact atctggagca gaagaacagc
     1021 cgatttctgg acagttccaa tcaggttgag aggatcgttg tcactatccg tgacaccacc
     1081 gaacgaaggc agttgcagga acaactcatc aaggcgcaga agatggaaag tctcggagtg
     1141 ttggctggcg gtgtggcaca tgatttcagc aaccttctga ccagcattgt cggaaactcc
     1201 agcctgatgc tctcggagat gccatccgag tctcctcttc aagagggtct caaacagatc
     1261 gcaaaggctg cagagatcgc ttcgggtctg tgtcggcaga tgcttgccta ttcaggcaag
     1321 gggcgttttg ttgtccttcc gattaacctg acggatgttg tcaatgaaat gaaagatctg
     1381 ctcgatgtca ctgtttccaa aacggtcgag atccggtacg agttggacac aagccttccc
     1441 tgcgttgaag cggatgccgc ccagatgtac caggttatct cgaatcttgt taccaatgct
     1501 tcggaatcta tcggcaatcg caaagggacg gtgacaatca aaacagcttc tcgcgaatgc
     1561 accagggagt tctttgcaga ctgctatctg aatctcgtct tgcaggaagg ttcctatgtg
     1621 atgcttgagg tttcggacac gggctgcggc atgacacccg agacgatgca gaagatgttc
     1681 gatcccttct tcacgaccaa gtttaccggt cgagggctgg ggctctccgc ggtgcttggt
     1741 attgttcgcg gtcattcagg cacgattcag gtcaacagcg aaccggggca gggcaccact
     1801 tttcgtctgc tgttgcccgc aaccgatcgg tgcccttcta atcatcagcc ggcgcacgta
     1861 gagtccaaga cgtttctgcg tgggcttaca attctggtcg ttgatgatga tccggcagtc
     1921 ctggatctca ttgagttaat tctggatgga gaaggttcga aagtgctgaa agcaacggat
     1981 ggtcttgagg cactggatgt ttatcaggct catgccaagg agatcgccct ggtgatcctg
     2041 gatatgacga tgccgaatct gtcaggggag gacacattgt ttcgactcag ggcgattgac
     2101 ccggacgtcc gggtgctgct ttccagtgga tattcagaga caccggagaa gttccgattc
     2161 gaagaatggg gtgcgaaggg ttttatccat aaaccctatc ccgtggctga cttgatcaag
     2221 gcaatcgagc ggtcgctgga ctgatattga caaggcagtc gttcctgcgg agcagattcg
     2281 tccagacaca gaaaaaacgt tgcgggcaaa acgcccgcac cgctattgta accgagaaca
     2341 aacgagatga agactatttc atcttgtaac gtttcttgaa acggtcaatt ctgcccgcgg
     2401 tatccacaaa tctctgcttt ccagagaaaa acggatggca gttggaacag atttcaacgc
     2461 ggatgttctg cttggttgaa cctgtttcga tgacgttccc acaaccacac acaatcttgg
     2521 tttggtggta ctctggatgg atatccttct tcacgacgaa atcaacctcc ttcactctgc
     2581 ggtaccaacg ggcaccttag cgacacaggc caatcggatt ctactcgctc ttgggtggct
     2641 cttgctgttg tgttgcctgg ggagcagcct tggccttgtt tctctccaaa agttccgcga
     2701 cgccaaacgc cattgaaaac aacaatgccg tgttggcgag gctctggaac gtggacggcc
     2761 ggatgccacc ttcgacgaac atgcggatcg gtctgtg
//
LOCUS       DAOQUC010000419         2795 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_42673_length_2795_cov_3.432482, whole genome shotgun sequence.
ACCESSION   DAOQUC010000419 DAOQUC010000000
VERSION     DAOQUC010000419.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2795
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42673_length_2795_cov_3.432482"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2474
                     /locus_tag="PKH07_08165"
     CDS             <1..2474
                     /locus_tag="PKH07_08165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34956.1"
                     /translation="SVDEVCSMTISGSLAQSLACAVLCSLYLMIYPVCARAQESSSEW
                     TERLLPLPKKISINGSATVPAGRMLVTMPEGQSPVLQTAKGILKPFNTGSNGFEIRMV
                     LTSDSDPSCPRSVLESLARVPNSDQAYAIEPQEHDGRFDGLLLVANTPVGLLYAARTL
                     SQMLPTEDQIGESVVFPGASVLDWPDLAERGQWGGNCTSDLPWLSERKMNVVEVHANL
                     GFDEQHHPTASLDQKTLDTAKRLGIKVVPIIMHLEQLAPTQMFQFFPELAATPDPHTS
                     LPTDFKPAVCFSQPKATEILSQWMKQILSTGEVTEIMVWLSEDAAPCFCTSCAGKEPY
                     VREVECIQNAFSQARMTRSDGALRLLTSQGSYPVNDRIIEVLNTDTKLSFYDGSKTYD
                     SSHKPMIIHLLEQFSASGRWLGVYPQLTNSWRSVFPFTGPQFIHTRMKEFADKKLHCL
                     IGYATPSNRFYEFNVTAAAEWSWNSKGRTPTEFAEVYARRKGVSNPDRFAEWAQIVGT
                     VGWDIAGSRVLLTLLYNPTMPVIPKDGKKSENILETIPDMVFGQGLLGEFPTKEHFEE
                     DIVLAERALHLARIEKSAEMIEESLSVLNTLRFLQGLKQMADAKTLSEEEALQAFVQG
                     TAQVREAARQVTGALYRWGAIVNPGALPSRYRDTVNVFSKTAAAATALSSSLGIEDPS
                     PEYSPRLIQEWKSEDFRNNPEAVLWADISDFVTSDGQFDVTFQFTSGAVGLEILSVAL
                     LKGASQEGATVFDETRSTFHVGKNDSWTEYWMQVPKDVFRTKTSEERLFLKCEVRGPA
                     LDLPSNRRTTKGQILIRKSWRDSTP"
     gene            2496..>2795
                     /locus_tag="PKH07_08170"
     CDS             2496..>2795
                     /locus_tag="PKH07_08170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34957.1"
                     /translation="MMNNREWIQRTLAHEDTGAVPYNFAFSPPARQKVEKHYGGSPLE
                     QILSFPIRMTGLNSIKPLYASRSEYGESIRDEYGVVWSTNDIDRGSPTGPCLLEPS"
BASE COUNT          677 a          762 c          728 g          628 t
ORIGIN      
        1 tgtctgtaga tgaggtttgt tcaatgacca tcagtggttc tctcgcgcag tctcttgcct
       61 gtgctgtgct gtgttctctg tatctgatga tttaccctgt ttgtgctagg gcgcaggagt
      121 cttcttcgga atggacagag cgactgctgc cacttccaaa gaagatcagc atcaatggaa
      181 gtgccacagt tccagcaggg cgcatgcttg tcacaatgcc agaaggtcag agccctgttc
      241 tgcaaacagc gaaagggatt ctgaaaccct tcaacacggg cagcaacggc tttgagatca
      301 gaatggttct gacatccgat tcagatccat cctgcccgcg ctccgtgctg gagtcactgg
      361 cacgagttcc gaactcggat caggcctatg ccattgaacc ccaagagcac gacggccgtt
      421 ttgacgggct tctgcttgtc gcgaatacac ctgtcggact cctctatgct gcccgaactc
      481 tgagccagat gcttcccaca gaggatcaga tcggtgagtc ggtagtgttt ccaggtgcat
      541 ccgttctgga ctggcctgat ctggctgagc gcgggcagtg gggtggcaac tgcacatccg
      601 atctcccgtg gctttccgaa cgaaaaatga atgtcgtgga ggtgcatgca aatctcggtt
      661 ttgatgaaca acatcatcca acggcgtctc tcgatcagaa gacactcgac acggccaaac
      721 gactgggaat caaagtcgta ccgatcatca tgcatctgga gcagctcgcc ccgacccaga
      781 tgttccaatt cttcccagaa ctggctgcaa ccccggatcc ccatacatcg ctcccaacgg
      841 acttcaaacc cgctgtctgt ttctcacagc ccaaggccac agaaatcctc tctcaatgga
      901 tgaaacagat tctttcgact ggcgaagtca cggaaatcat ggtctggctt tctgaagacg
      961 cagcgccctg cttctgcaca agttgcgctg gcaaagaacc ttacgtccga gaagtggagt
     1021 gcatccagaa cgcattcagc caagcccgaa tgacaagatc agacggggca cttcgactgc
     1081 tgacgtctca aggcagctat cctgtcaatg accggattat tgaagtgctg aatacggaca
     1141 ccaaactcag tttctatgac ggttcgaaga cctatgacag ttcccacaag cccatgatca
     1201 ttcacttgtt ggagcagttc tcggcatccg gtcgctggtt gggagtgtac ccacaactga
     1261 caaactcctg gcgttccgtc tttccgttca cgggtcccca gttcatccat acccgaatga
     1321 aggagtttgc cgacaagaaa cttcattgcc tcatcggtta cgcaaccccg tcgaatcggt
     1381 tctacgaatt caacgtgacc gcggcagctg aatggagttg gaacagcaag ggcagaacgc
     1441 caactgaatt cgcggaagtc tacgctcggc gaaaaggcgt ttccaaccct gaccgttttg
     1501 cggaatgggc tcagatcgtc ggcactgtcg gttgggatat tgctggaagc cgggtactcc
     1561 tcactcttct ttacaatccc accatgcccg tcattccgaa agatggaaag aagtcggaaa
     1621 acatcctaga gacgattccg gacatggttt tcggacaggg cctgctcgga gaatttccaa
     1681 cgaaggagca ctttgaagag gatattgttc tggctgaaag agccctgcat ctggctcgta
     1741 tcgaaaaaag tgcggagatg atcgaagagt cgttgtccgt cctgaacacc cttcggtttc
     1801 tccaaggtct gaagcagatg gcagacgcca agacgctttc tgaagaagaa gcgctccagg
     1861 ctttcgtgca gggaaccgca caggttcgag aggccgcacg gcaagtcaca ggagcactct
     1921 accgttgggg agcaattgtg aatccaggtg cccttccttc ccggtaccgt gacacggtca
     1981 acgtcttctc gaaaacagcg gctgccgcca cagctttgtc gtccagcctt ggcattgaag
     2041 atccaagtcc cgaatacagc cctcggctta ttcaggaatg gaagtctgaa gattttcgca
     2101 acaatcctga agcagttcta tgggcggata tttcagactt cgtgacaagc gatgggcagt
     2161 ttgacgtcac cttccaattc acctcggggg cggtggggct ggaaatcctt tcggtggcac
     2221 tgttgaaagg agcctcacag gaaggcgcca ccgtcttcga cgagacacgt tcaacctttc
     2281 acgtcggaaa gaatgactcc tggacggaat actggatgca agttccgaaa gatgtattcc
     2341 gcacgaaaac gtccgaggaa cgcctgttcc tcaaatgtga agtcagaggc cctgctttgg
     2401 atttgccttc caaccgtcgc accacgaagg ggcagatcct gattcgaaaa tcctggcgcg
     2461 acagcactcc ttgagaagat gaggcattcg aaaagatgat gaataatcgg gaatggattc
     2521 aacgtacatt ggcacatgag gacactggcg ctgtcccgta caatttcgcg ttttcaccac
     2581 cggcaagaca gaaggtcgag aagcactacg gtggctctcc tttggaacag atactcagtt
     2641 tccccatccg catgacggga ctgaattcga tcaagcccct gtacgcctcc cgaagtgaat
     2701 acggggagag catacgagat gaatacggcg tcgtgtggtc taccaatgac attgatcggg
     2761 gttctcccac aggcccatgc ctcctcgagc caagc
//
LOCUS       DAOQUC010000420         2790 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_42791_length_2790_cov_3.741865, whole genome shotgun sequence.
ACCESSION   DAOQUC010000420 DAOQUC010000000
VERSION     DAOQUC010000420.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2790)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2790)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2790
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42791_length_2790_cov_3.741865"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            165..509
                     /locus_tag="PKH07_08175"
     CDS             165..509
                     /locus_tag="PKH07_08175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34958.1"
                     /translation="MAEIRKAQTLKDWYIAFFEGAEIYSDEPVPRFLGRISDRLSPDS
                     IFNEIGPYGDRFGAVSIWNEFGNYGGQFSPYSPFNQFGTSPPFIIKDNRRIGRLTINE
                     FFQGAISPYWLK"
     gene            722..1654
                     /locus_tag="PKH07_08180"
     CDS             722..1654
                     /locus_tag="PKH07_08180"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HNT34959.1"
                     /translation="MTSAMLLANQNVEPSGSQVHKEDYFLEPGDTLELRFFYEPDLKE
                     SEYRLKPKDSFKVVSFRNPELDREVTVRPDGRVALPLIGEITAEGLTPEEIRDHIASC
                     YKPILKEPDVTVDVTGFQGQKTYNRTALSMSGTQTFTIRPDGKVSLQLIGELSAAGKT
                     PAELSETILAQYEPLLKDPRATLVVTGFSIQKLYVGGEVIAPGVIPLNKFGQTTVLQA
                     ISHSGGFRETADPRSVIVIRRAPGSETVIKKLNLTAVLKGETLEDDIPLQALDVIYVP
                     KTFIAEADKFVEQHVRQLVPVDLQAAFTYGLVKN"
     gene            1726..2334
                     /locus_tag="PKH07_08185"
     CDS             1726..2334
                     /locus_tag="PKH07_08185"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HNT34960.1"
                     /translation="MRRLCAKILTGSEKQHSTVIMVSSCDRGEGTTTVATNLARTFVQ
                     EAGGQVLLIDANLRHPALHTLFGFDMEPGLSDVTRNGTQVKDAIQESKADSLHVLSAG
                     TQIAYPGLVFNSPAMDSCLEQIRKKFRVIVLDAPPIVPYSDSLYLAKKADIVLLVIEA
                     EHTRWEVAQEAIQKLDYVGVQLYGTILNKKRFYIPSFLYRAL"
     gene            2425..>2790
                     /locus_tag="PKH07_08190"
     CDS             2425..>2790
                     /locus_tag="PKH07_08190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34961.1"
                     /translation="MKNLLRTTSLRMFKLLDIASIAVSFILAIIISTRRLEASELLHT
                     QIQVSDIVAFLVLLFGWHMVFLWGHLYESRRIGTRAGEYYEILKCITMGSVLVLALGV
                     FLHWEILNKSFFAWFWGLLL"
BASE COUNT          664 a          677 c          749 g          700 t
ORIGIN      
        1 tacaaattgg aatctgaaca ttcctataga gagctggtag ggcattgctg catagtagta
       61 ggtgtgtaca cacgttccca atcgttatcc ttgtctgcgt gggatgtgcc cgaggtttct
      121 atccttcccc gggaaatgaa gatgatcgca cacatcagtg gcgcttggcg gaaattcgaa
      181 aggcacagac tctcaaggat tggtatattg ccttttttga gggtgctgag atttacagtg
      241 atgaacctgt cccaagattt ctgggaagga tttctgaccg actcagccct gattcgattt
      301 tcaacgagat tggaccctat ggagaccgtt tcggagcggt cagtatttgg aatgaattcg
      361 gcaattacgg agggcagttt tcgccataca gcccgttcaa ccagttcggc acctctcccc
      421 cttttatcat caaggacaac aggaggattg gtcggctgac aatcaatgag ttcttccagg
      481 gagcgataag tccgtactgg ctgaaatgac gtatgtcaga gggtactcag actccatgtt
      541 gtaagggtgc acgggcagtc cgaacttggg gagtatatat gattggctgg aggttgagat
      601 gaaggattgc agtccaaaac cgagagtggc acggtggctt gcagagggtc gttgttcatg
      661 tgtgttggtt ctactgctgg ttcttactgt cttttccgca tgtgtaaaaa ggcctccgga
      721 tatgacatct gcaatgcttc tggcaaatca gaatgttgaa ccgagtggat ctcaagttca
      781 caaagaggac tatttcttgg aaccagggga cactctcgaa cttcgcttct tctatgagcc
      841 cgacctgaaa gaatccgagt atcgcctgaa acccaaggat tcctttaagg tggtttcgtt
      901 tcgaaacccg gagttggaca gagaagtgac cgtccgtcct gatgggcgag tcgcgcttcc
      961 cttgatcggc gagatcactg cagagggcct cactcccgag gagattagag atcacattgc
     1021 ttcctgctac aagccgatcc tgaaggagcc tgatgtgacc gttgacgtca cgggattcca
     1081 gggacagaaa acctacaacc ggacagcgct ctccatgtcc ggaacgcaga catttacgat
     1141 tcgccccgat ggaaaggttt cgcttcagct catcggtgaa ctgtctgctg caggcaagac
     1201 gccggcagaa ctcagcgaga caatcctggc tcaatacgag cccctgttga aggatccgcg
     1261 cgcgacgctt gtggttacag gtttctcgat ccagaaactc tatgttggag gagaggtcat
     1321 cgctccggga gtcataccgc tgaacaagtt tggtcagaca acggtactgc aggcgatctc
     1381 ccattccggt ggttttcgtg aaaccgctga ccccagaagc gtcatcgtca tcaggcgtgc
     1441 tccaggcagt gaaacggtca tcaagaagtt gaacctcaca gccgttctga agggagagac
     1501 actcgaagac gatatccctt tacaggcgct tgacgtgata tatgtcccca agactttcat
     1561 tgctgaagca gataagtttg tcgaacagca tgtccgtcag ctggtacccg ttgatcttca
     1621 ggctgcattc acctatggcc tggtcaagaa ctgagaggcg cacaagagat gtttggaaaa
     1681 tcggttcccc tgaaaacgtt gggctccgca caaggtgtgg aggagatgag gcgactgtgt
     1741 gcgaagatac tcacaggttc agagaagcaa cactccactg tgatcatggt ctccagctgt
     1801 gatcgtggtg aaggaacaac tacggttgca acgaaccttg ctcgcacgtt cgtccaggaa
     1861 gcaggaggcc aggtgttgct cattgatgca aacctccgac atcctgcgct ccatactctt
     1921 ttcggattcg acatggaacc gggtctgtcg gatgtcaccc gaaatggcac acaagtgaaa
     1981 gatgcgattc aggagtcgaa agccgacagc ctgcatgttc tgagtgctgg tacacagatt
     2041 gcctatcccg gtctggtttt caactctccc gccatggaca gttgtcttga gcaaatcagg
     2101 aagaaatttc gggtcatcgt gctagatgca ccgccaattg tcccttactc ggattccttg
     2161 tacctggcaa agaaggcgga tatcgttttg ctggtcatcg aggcggagca tacacgctgg
     2221 gaagtcgctc aagaggctat ccagaaactg gattatgtcg gggttcagct ctatgggacg
     2281 attctgaaca agaagcgatt ctacattcct tcgttcttgt atagagctct gtagattgtt
     2341 gagagataga gaggctgacg gcactggcca cctgaggtct ttcccgatgc gtttgccgaa
     2401 tgcacagcag acaattggac gactatgaaa aacctgttac gcacaacatc gctgaggatg
     2461 ttcaaactcc tggacatcgc cagcatcgcc gtttcattca tcctggccat catcatctcc
     2521 accaggcgtt tggaagcctc tgaacttctg cacacccaga ttcaagtgtc agatatcgtg
     2581 gcattcctcg tgcttctttt tggttggcat atggtcttcc tctgggggca cctctacgaa
     2641 tccaggcgaa tcggcacaag agcaggagaa tactatgaga tactcaagtg catcacgatg
     2701 ggcagtgttc tcgtcttggc gcttggtgtg tttctccatt gggagatcct gaacaagtca
     2761 tttttcgctt ggttctgggg gctgttgctc
//
LOCUS       DAOQUC010000421         2789 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_42818_length_2789_cov_2.864301, whole genome shotgun sequence.
ACCESSION   DAOQUC010000421 DAOQUC010000000
VERSION     DAOQUC010000421.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2789
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42818_length_2789_cov_2.864301"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..816)
                     /locus_tag="PKH07_08195"
     CDS             complement(<1..816)
                     /locus_tag="PKH07_08195"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNT34962.1"
                     /translation="MRKSSELLGQFLQHIQGAQHVSEKTVEAYRRDLIQFFNYLEGDW
                     SGRIPCQVVEDHFPETDISEVDELRIRGFLSYLHESGSSSQTVQRKMSAIRSMLRYLA
                     AGGLIPSNPADRVRTMKMGRHLPRALSQEGAEQLIESPEENEFCQVRDRAILETLYST
                     GMRVTPLTRINVADYDSRVGSVRIVSKGSKEQILPLGQCALQALDEYRKVRSNLLKQL
                     GKPEVDALFLNRVGERITQRNVQRLTRKWALKTGLGSVTPHTLRHSFATHLLEN"
     gene            complement(855..>2789)
                     /gene="topA"
                     /locus_tag="PKH07_08200"
     CDS             complement(855..>2789)
                     /gene="topA"
                     /locus_tag="PKH07_08200"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HNT34963.1"
                     /translation="AITPATVREAVEHPGPIDMSKVNAQQARRVLDRLVGYKISPLLW
                     RSVGKGLSAGRVQSVAVRLICEREDAINAFVPVEYWSIVAQLCHDTGAPFNAYLYAIG
                     EARVGKEDGDLKDAKVRRIATGEEAEDLVSRLKGEAYRVVNVDKKPKNRSAAPPFITS
                     TLQQEASRRLGFSPDRTMRIAQRLYEGVEIGSDIVGLITYMRTDSVRISEEGLTEARQ
                     YINTQIGQDYLPEKANRYRQKSSAQDAHEAIRPNYVSRTPEEMKVYLEKDELRLYELI
                     WRRFVASQMVPAQFMATTVDISAGDCMFRATGLQLLFEGFLKVYGRNAEDEEEGMLPE
                     LSPQDLLKLLSLRSEQHFTKPPARYNEASLIKELEDKGIGRPSTYASIVKTIQDRGYV
                     EKKERVFFPTELGFLTNKLLIEAFPEILDVGFTARVEDRLDKVEEGNEDWQALLSGFY
                     TPFSKAVDSALAGLRKSIKDMQEETEEVCEKCGKKMVKKWGRNGWFLACPGYPDCKNA
                     KALNGNGTQQVRETGEPCPECGKPLLIREGKRGEFYACSEYPKCKYSKSIGIGINCPK
                     PDCDGQVTALKGKRGKLFYGCTNYPKCDFIAWDEPVVEKCPECGNPFLVRKEYKSKGS
                     VIKCPQKECKYLRPVEPKPD"
BASE COUNT          607 a          820 c          675 g          687 t
ORIGIN      
        1 attctccaag aggtgtgtcg cgaaagagtg cctgagagta tgcggagtaa cacttcccaa
       61 accggtcttc aacgcccatt tccttgtcag gcgctgaaca ttgcgttgcg tgatacgctc
      121 gcccactctg ttcaagaaga gagcatcaac ttccggcttg cccagttgct taagcaaatt
      181 ggagcgaacc tttcgatact catccaaggc ctgaagcgcg cactgaccca gcggcagaat
      241 ctgctccttg ctccccttgc tgacaattcg cacgcttccg acccgtgagt cgtagtcagc
      301 gacattgatt ctggtaagtg gagtcacgcg catccccgtg gagtacagag tctccaagat
      361 cgccctgtcg cgcacctgac agaactcatt ttcttcaggc gactcgatga gctgctctgc
      421 tccctcttgg ctgagtgctc tggggagatg cctgcccatc ttcatcgtgc gtactcgatc
      481 tgcgggattg ctcggaatca gcccccccgc agcaagatat ctcagcatgg accgaattgc
      541 agacatcttc ctttgcacag tctgggaact ggaacccgat tcatggagat aggaaaggaa
      601 cccccgaatt ctcaactcat ccacttcgga aatatctgtc tcaggaaagt ggtcttccac
      661 gacctggcac ggaatgcgcc ctgaccagtc accttccaga taattgaaaa actggatcag
      721 gtcgcggcgg tacgcttcga ccgttttctc cgaaacatgc tgagcgccct gaatatgttg
      781 cagaaactga cccaacagct cggaactttt cctcacaacg atctcccgac aaagggtcac
      841 tcggaaggtt tctctcaatc cggctttggt tcgaccgggc gcagatattt gcattccttc
      901 tgaggacact tgatgaccga tcccttggac ttgtactcct tgcgaaccag aaatggattt
      961 ccacattcag ggcacttctc caccaccggc tcatcccacg caatgaagtc gcacttgggg
     1021 tagttggtac aaccgtagaa taatttcccc cgcttgcctt tgagtgcggt gacttgcccg
     1081 tcacagtcag gcttgggaca gttgatacca atgccaatgg acttcgagta tttgcatttg
     1141 gggtactccg agcaggcata gaactccccg cgtttgcctt cgcgaatcag taggggctta
     1201 ccgcattcgg gacacggctc gcccgtctcg cgaacttgtt gggttccgtt gccattgagc
     1261 gccttggcat tcttgcagtc cggatatccc gggcaggcaa gaaaccagcc attgcgaccc
     1321 catttcttga ccatcttctt gccgcacttc tcacaaactt cttctgtctc ttcctgcatg
     1381 tccttgatgg acttgcgaag tccagccaat gcagaatcaa cagcctttga gaatggtgtg
     1441 tagaaaccgg aaagcagcgc ctgccagtcc tcattccctt cctcaacttt atccagacgg
     1501 tcttcaacac gtgccgtaaa gcccacatcc aggatttccg ggaacgcttc gatcaggagt
     1561 ttgttggtca aaaagcccag ttccgtcggg aaaaagaccc tctccttttt ctcaacatag
     1621 ccccgatcct ggatcgtctt cacgatggaa gcataggtgc tcggacgccc gatgcctttg
     1681 tcctccaact ccttaatcag gctcgcttca ttgtatcgcg ccggtggttt ggtgaagtgc
     1741 tgctccgatc tgaggctcag caacttcaga aggtcttgtg gtgaaagttc gggaagcatt
     1801 ccctcctctt cgtcttctgc atttcgacca tacaccttga gaaagccctc gaagagcagt
     1861 tgaagaccag tggcacggaa catacagtcg cccgctgaga tgtcaaccgt agtcgccata
     1921 aactgcgctg gcaccatctg cgaggcgaca aagcgacgcc agataagctc atagaggcgc
     1981 agttcgtcct tttccaagta aaccttcatc tcttcaggcg ttcgggagac atagttggga
     2041 cgaatagcct catgagcatc ttgtgcggaa ctcttctgtc tgtaccgatt cgccttttcg
     2101 ggcagataat cctgaccgat ttgtgtgttg atatactgcc tcgcctcggt aagtccttct
     2161 tccgaaattc gaactgagtc cgttctcatg taggtaatga ggccgacaat atcggaaccg
     2221 atctcaacac cctcatacag gcgttgcgca attctcatgg ttctgtcagg actgaagccc
     2281 agacgtctcg aagcttcttg ctgcagcgtg cttgtgatga acggtggtgc ggcgctgcgg
     2341 ttcttcggct tcttgtccac attcacaaca cggtacgctt cacccttcag acggctgacg
     2401 agatcctcag cttcttcgcc cgtggcaatc cgtcgaacct tggcatcctt gagatctccg
     2461 tcttccttcc ccacacgcgc ttcaccaatg gcatagagat aggcgttgaa gggtgctccg
     2521 gtatcatgac acagttgggc gacaatagac caatactcga cagggacaaa tgcgttgatg
     2581 gcatcctcac gctcgcagat gagccggacg gcaaccgact gcactcttcc cgccgacagt
     2641 cccttaccga cactgcgcca caacagagga ctgatcttgt atccaaccag acggtctaat
     2701 acgcgtcgtg cctgctgcgc attgaccttg gacatgtcaa tcggtccggg atgctccacc
     2761 gcttccctga ctgtagccgg tgtgattgc
//
LOCUS       DAOQUC010000422         2787 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_42860_length_2787_cov_3.259883, whole genome shotgun sequence.
ACCESSION   DAOQUC010000422 DAOQUC010000000
VERSION     DAOQUC010000422.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2787
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42860_length_2787_cov_3.259883"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..523)
                     /locus_tag="PKH07_08205"
     CDS             complement(<1..523)
                     /locus_tag="PKH07_08205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34964.1"
                     /translation="MRTKKTTKKTTKKPAAAKKPTAKKPAAAKKPTAKKPAAAKKPTA
                     KKSTKKANKRKHYATLPSHDQAIRRMAKASGLSMTKTKELLSKAAKGAKGKDRVEPPK
                     RKTHKMNKRATPSLSKAHDRVARDLHVSPSLVHRTMTQHSQRPHYNRNPLDAIYAPNP
                     TFSAGQTAAAIITT"
     gene            complement(1179..2045)
                     /locus_tag="PKH07_08210"
     CDS             complement(1179..2045)
                     /locus_tag="PKH07_08210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34965.1"
                     /translation="MSTWFLHPRNFQEKIHLPLDFSDPELGFFHVTSHAARLQKSWQY
                     RSRLELRSEGIVGLSGGPQDAPNKISFILELDRAKTLLHAIRTMSRACHDQISIRDLL
                     STMFDWLDFPDCSGWEFAMMNVFGLDEESCSMVDYNEDTESFLKSLLNAILVDESLVS
                     PELLTSSGWRNLLKGLGKDWENQFIYPINRYDAARAFETVLEKVLGDEYVGAGEIPGI
                     CVPIVGFVAPFEDFLRVQPDDICITQAAVKEGASIETNYGECEVRLFPKEVKLVAMRV
                     EERGIFMEDQTD"
     gene            2227..2580
                     /locus_tag="PKH07_08215"
     CDS             2227..2580
                     /locus_tag="PKH07_08215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34966.1"
                     /translation="MEDVAQEIVDAIKPAIVAALPEITNKMVDEAIPRIKQEIPQLVS
                     VATSHLQEEMKKLQPQLTEMAIASAHEVLADEQVQREVAAKTKEIRDNIRTGFVASTL
                     TIVAVILFATWYGKR"
BASE COUNT          638 a          713 c          731 g          705 t
ORIGIN      
        1 ccgtcgtgat gatcgccgct gcggtctgac ccgccgagaa cgtcgggttg ggggcgtaga
       61 tggcatcgag tgggtttcga ttgtaatggg gacgctgaga gtgttgcgtc atggtccggt
      121 gaaccagcga aggactcacg tgcagatcgc gcgcgacgcg atcatgggcc ttgctcagag
      181 aaggcgtggc acgcttgttc atcttgtgcg tctttcgctt cggtggttcg acgcgatcct
      241 tgcccttggc tcccttggca gctttgctga gtagttcttt ggtcttggtc atggagaggc
      301 cagacgcttt ggccatgcga cgaatggcct gatcgtgcga agggagcgtg gcgtagtgct
      361 tgcgcttgtt ggctttcttc gtcgacttct tggcggtcgg cttcttcgca gcggctggct
      421 tcttggcggt cggcttcttc gcagcggctg gcttcttggc ggtcggcttc tttgcagcgg
      481 ctggcttctt ggtggtcttc ttggtggtct tcttggttct catggacgtg tcctcagtgg
      541 ccctcgcatt tctggaagga gagccttgtt tgagttgttt tttgacggtc gaaagcttgg
      601 ctgatgagtg ggctcgattg tgtttggaaa agttcttctt cgcgtcttcg attcgacgcg
      661 acagcccttg caattttttt cgataagagg ctgcatcggc tgtctcgcgc cagcccaaag
      721 aatcaaacca cagaggagac gaaagcacct tggagacggc ttcgtcggca aggtctagat
      781 cctctgggga gacatccagt gtctctacag tggagatgaa gtgcgggcgc gtctcggtat
      841 ctccaggtat atgcctgaag ttccgagtga gcgcggccaa gagaatttca gacacagcgg
      901 gtgtcatgaa gatcgcttct tctagcgggc gtccgatctt ctcggattcc ttcgatgcaa
      961 ccaccttgcg aagccattgc accaattttg tcttggagac cttcccagaa gaagcaaggt
     1021 cgtccacgaa gtccattgcg ccatcagaat cgaatggtcc agtaccccac gttcccatgg
     1081 aatcacgctt gccctttccg acaaaaagat gaaattctcc acggtcctga gacttgcgag
     1141 ttcggtgcgg aggagaagaa ccgtgtcgac atgtttgatc aatccgtctg gtcttccatg
     1201 aagataccac gttcttcgac gcgcatagcg accagtttca cttctttcgg aaagagcctc
     1261 acttcacatt caccatagtt cgtctcaatg gatgcgcctt ctttcacagc agcctgtgtg
     1321 atgcaaatgt catccggctg gacacgtaga aaatcttcga atggcgcaac aaatccaaca
     1381 attggaacgc aaatgcctgg tatttcacca gcgccaacat attcatcgcc tagcactttt
     1441 tccaacactg tttcaaacgc cctggcggca tcatatctgt ttattggata aataaattgg
     1501 ttctcccaat ccttgcccaa tcctttcaac aaatttctcc acccagagga tgtcagcaat
     1561 tctggagaaa ccagcgattc gtccaccaag atcgcattca agagagactt caaaaacgat
     1621 tccgtgtctt cattgtaatc aaccatgctg cacgattctt cgtccaagcc aaatacattc
     1681 atcatggcga attcccatcc actacagtct gggaaatcca accaatcgaa catggtcgac
     1741 aacaagtctc gaattgaaat ttgatcgtgg caagcacgac tcatggttcg aatcgcatgg
     1801 agcaacgttt ttgcccgatc caattcaagg atgaatgata ttttgttggg tgcgtcctgg
     1861 ggaccaccac tgagcccaac gataccttcg ctccgcagct ctagacgact tctgtattgc
     1921 cacgattttt gtagccttgc tgcgtgactc gtgacgtgaa agaatccgag ttctggatca
     1981 gaaaaatcga gtggtagatg gatcttttcc tgaaaattcc ttgggtgaag aaaccaagtt
     2041 gacatactca atgagtaaca tactcatgag cgtgtatccc taaattaaaa acacaagcgc
     2101 aatggattcg tccattctaa aggcatgatg cataccctgt gcagaccgac catttcgcca
     2161 ttcatgctgg aacgaccgat tttgatcttg gcagaacaac cgtcgaacga cgacaagacc
     2221 aaccttgtgg aagacgttgc tcaagagatt gtcgatgcaa tcaagccagc catcgtggcc
     2281 gcgctaccag agatcaccaa caagatggtg gatgaagcga tcccgaggat caaacaagag
     2341 atcccacaac tcgtgagcgt ggccacgagc catctgcaag aagagatgaa gaagttgcag
     2401 ccacagctca cagagatggc catcgccagc gctcatgaag tgctcgctga tgaacaagtt
     2461 caacgagagg tcgccgcgaa aacgaaggag atccgagaca acatccgtac tggatttgtg
     2521 gcctctacgc tcaccattgt cgctgtgatt ttgttcgcaa cttggtatgg taagcggtga
     2581 ctctccgctg aatctatgaa tccttcaacc gggtccgggt ccggggccgg ggccaacttg
     2641 cgggcgagct tctgtggctg ggcaaagttc gcagttgacc ctacgaacgg cacgtttttc
     2701 agcgagagaa acctctggcc tgttcacgat gtccttgagc gcagacctga gttgcaattg
     2761 gagagttttg ctgatgggga actctgg
//
LOCUS       DAOQUC010000423         2787 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_42868_length_2787_cov_2.710469, whole genome shotgun sequence.
ACCESSION   DAOQUC010000423 DAOQUC010000000
VERSION     DAOQUC010000423.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2787
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42868_length_2787_cov_2.710469"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1064
                     /locus_tag="PKH07_08220"
     CDS             <1..1064
                     /locus_tag="PKH07_08220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34967.1"
                     /translation="GCLNDLLFLLLVWLVWLGTKQRLSTRGFWKQITFLTVPAAVIAS
                     PVFIKNWILTGNPFFPILAGVLGRNRPGLEITHAHVMDHTPPAAIFTPLGFLKWFAVR
                     WTGVNYAGTFVLNLAAIALLTTLLMLLCRRQNWKEFPQWIRFHVLFIAAAWVMNLLCC
                     NNMDGRFFLPIVPAICVQVSLWLCCEAWGHLPGGRQRLRPLLLLCLVVLSLFGFIMER
                     SRFAETMNESLVPVLTESARSQYYKDRIPKYDVVEYVNANLPQDSCILSIGYPARRRT
                     IGTACGAPNPVNAAIGTDTYTADDLLRAFRLLGVTHFVHPGSNALQPEAVDGLIEQGL
                     GKEVFRSEQGYALFAIANQ"
     gene            complement(1251..1829)
                     /locus_tag="PKH07_08225"
     CDS             complement(1251..1829)
                     /locus_tag="PKH07_08225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34968.1"
                     /translation="MKHPEIILLPVLMLADYYLTLYSDYLRLKKHSDYFDLESFELNL
                     GMQKEIRNRRLFSPKHCIGVVFMTLALWCTSLAEEGVYEFILGFFLVTFGILLSRHIA
                     NIVMFDFVRRNAETIHGKISVSYEFSLCSSRSSISVSLIPLILVSVFRPSIFVFGGIL
                     ANLNLIYVHFAWGRRHRKRRIRSRELQDEARS"
     gene            complement(1897..>2787)
                     /locus_tag="PKH07_08230"
     CDS             complement(1897..>2787)
                     /locus_tag="PKH07_08230"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNT34969.1"
                     /translation="ADAMTDLRTVLSLVPDDTETTRNLRTAKFLLGLSLARQNKYQLA
                     YDVFQELLQGDPDSVEIAHNVALLAAACEKYAEAVRMWKALFAHYQAQLDKVSEDEYL
                     AHLIVEIQKKIAENSARIPAEKPEEKTVFAPQAPDAKTGVKLAVSLMEQKLYAQALQI
                     LERAMSEGDNSLDVVDNLGWAYLHMGNIEESFRLWRKGLRDHNQHPRLKDSIIRGHVH
                     VGRSMLEGNHITPALTHLKQALAADSKNIEAYDLLAQIYLQKGDPMAAIDLWTKALKI
                     DPRDKKIQRSIRAAKIRTRR"
BASE COUNT          638 a          722 c          711 g          716 t
ORIGIN      
        1 gtggctgctt gaacgacctt ttgttcttat tgctggtctg gctggtttgg ctggggacga
       61 agcaacgctt gagcacaaga ggattctgga agcagattac attcctcact gtgcccgcag
      121 cggtcatcgc ctctcccgtg ttcatcaaga attggattct gacaggcaat ccgtttttcc
      181 cgatcctggc tggcgttttg ggcaggaacc gacccggact tgagatcact catgcccatg
      241 tgatggatca tacacccccc gccgcgatct tcactccatt ggggtttctc aaatggtttg
      301 ctgtccgatg gacgggggtc aactatgcgg ggacttttgt cctcaacctc gcagccatcg
      361 ccctgctgac aacacttttg atgctgctgt gtcggaggca gaattggaag gagtttccgc
      421 agtggatccg attccatgtt cttttcatcg ccgcggcgtg ggtgatgaat ctgctttgct
      481 gcaacaacat ggatggtcgc ttttttctgc ccatcgtccc tgcaatttgc gttcaggtga
      541 gcctctggct ctgctgtgaa gcctggggtc atcttcccgg cggtcggcag cggctgaggc
      601 cgctcttgtt gctgtgcctg gttgttctca gccttttcgg ttttatcatg gaacgctctc
      661 gattcgccga gaccatgaat gagtccctgg tgcctgtgct gactgagtct gctcgaagtc
      721 aatactacaa agaccgcatc cccaagtatg atgttgtgga atatgtcaat gccaatctac
      781 cacaagactc ctgcattctt tcgatcggct atcccgcacg gagacgcaca atcggaaccg
      841 cctgtggtgc acccaatccg gtcaacgccg ccattggcac agatacctac acagccgatg
      901 accttctacg cgcctttcga ctgctcggag tgactcattt tgtgcatcct ggatcgaatg
      961 ctctgcaacc tgaagcggtg gacggcttga ttgagcaagg gctggggaag gaagtcttca
     1021 gaagtgaaca gggctatgct ttgttcgcca tagccaacca gtagaggcaa atccgctgat
     1081 acactctcaa cgtctgctca gactcctctt gtatgcccaa gacttgcttg cgtcatagtt
     1141 gccgactaac gcacctcagg gaagccgcat attgagcgtc gagaagacac tgaaacagaa
     1201 cagacatcat gtgcttgact gatcaatcag aaagcaagga actcattgcc ctatgaccta
     1261 gcctcgtcct gcagttcgcg agacctaatc cttctttttc gatggcgacg tccccaggca
     1321 aaatggacgt aaatcagatt gagattcgcg agaatgccgc caaagacaaa gatactgggt
     1381 cgaaagacag agaccagaat cagaggaatc agggagactg aaatcgagga tctggaacta
     1441 cacaaactaa actcatagga caccgagatt ttgccatgga ttgtctcagc attccttctc
     1501 acgaaatcaa acatgacgat gttggcaatg tgtctactca gcaagatgcc aaaggtcacg
     1561 aggaaaaaac cgaggatgaa ttcatacaca ccttcttcag ccaaagaagt acaccagagc
     1621 gccagtgtca tgaaaacaac gccgatgcaa tgctttgggc tgaaaagtcg tctattcctg
     1681 atttccttct gcataccgag attgagttcg aatgactcca aatcaaagta gtccgagtgt
     1741 ttcttcagtc tcaggtaatc tgaatacagt gtcagatagt aatctgcaag catcagaacg
     1801 ggtaacaaga tgatctccgg atgcttcatc ggacttcctc ctcagagttt gtcgctctcg
     1861 gtctacaaag aagaaacgtc gtcggtgtcg ttatgactat cttctcgttc ttatctttgc
     1921 cgctcgtatg gatcgttgga ttttcttgtc tcgcggatcg atcttgagag cttttgtcca
     1981 aagatcaata gccgccattg gatctccctt ttgcagatag atctgggcaa gaaggtcata
     2041 ggcttcgatg ttcttggagt ccgcggcaag cgcctgtttg agatgtgtca gcgcaggagt
     2101 gatatgattg ccttcaagca tacttctgcc aacgtgaaca tggccgcgga tgatgctgtc
     2161 tttgagtcgg ggatgctgat tgtgatcccg aagtcccttg cgccagagtc ggaaagactc
     2221 ttcgatgttg cccatgtgga gataagccca accgagattg tccacgacat caaggctgtt
     2281 gtcgccttcg ctcatcgccc gttcaagaat ttgaagcgcc tgggcgtaga gtttctgttc
     2341 catcaacgag acagccagtt tgacgccggt cttggcatca ggcgcttgtg gcgcaaagac
     2401 ggtcttctct tctggcttct cggctggaat tcgcgctgaa ttctcagcaa tcttcttctg
     2461 aatctcaacg atgagatgcg cgagatactc gtcttctgaa accttgtcca actgtgcctg
     2521 gtagtgcgcg aagagcgctt tccacattcg gacggcttca gcgtactttt cacaggccgc
     2581 tgccaggagc gccacgttgt gggcgatctc aacgctgtcg gggtcccctt gaagcaactc
     2641 ctgaaaaaca tcgtaagcaa gctgatactt gttctgtcga gcaagagaca gtcccagcag
     2701 gaatttggca gtgcgaagat tccgagttgt ctcggtgtcg tcaggaacca gactgagtac
     2761 cgtccgcaga tccgtcattg cgtcagc
//
LOCUS       DAOQUC010000424         2786 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_42885_length_2786_cov_2.927865, whole genome shotgun sequence.
ACCESSION   DAOQUC010000424 DAOQUC010000000
VERSION     DAOQUC010000424.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2786
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42885_length_2786_cov_2.927865"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1173
                     /locus_tag="PKH07_08235"
     CDS             <1..1173
                     /locus_tag="PKH07_08235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HNT34970.1"
                     /translation="DGSLKQLLQSRDSRTGQWLASPMKHPMRGKRRPVDNVHWLEVIG
                     AHANNLKNVDVRFPLQRLSVVTGVSGSGKSSLVRDVLSRACRAKLNNKRIPKNCCKNL
                     LGWKSLSRTMEVDQTPIGRTSRSCVATYVGLMDDIRNLFAMTSEARMLNYGAGRFSFN
                     VPGGRCEKCLGQGSIDVEMSFLPNVAVPCEECEGKQYNEDTLKVHFKGKNIAQVLSMS
                     VEEALEFLAGFSSLTEPLSFLRDIGLGYLTLGQTSPTLSGGEAQRIKLTSELSRRSRT
                     PCCYFIEEPTTGLHGSDVIKLLNVLHALVDKGHTLVVIEHHLDVIAEADYVVDLGPEA
                     GQEGGWVVASGIPEEIAEIEHSHTARFLSKVLKHKPKRITRPQMPSGLGKRPLRQK"
     gene            complement(1118..2368)
                     /gene="amrB"
                     /locus_tag="PKH07_08240"
     CDS             complement(1118..2368)
                     /gene="amrB"
                     /locus_tag="PKH07_08240"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HNT34971.1"
                     /translation="MTDFPKLRPVEAFQAQVSGKDVVCVRDATGFVDHIIAVPVPVFY
                     LFPFMDGNHSIRDIQAEFMRTTGEMVTSEQVAEIIASLDEHYLLDTERFAVHRENAMR
                     DFSNETIRKATHAGLSYESEPEALKRQLDTYFENIRDRQDSRRLSSRAHITGLVVPHI
                     DIRAGGPCYASAFKCLSSATQSPERVVILGTAHRGLENLFAATRKDFETPLGISATDQ
                     AFLDRLSEVFGDSLFSDEFAHRAEHVIEFQVTFLQHSFSSRVKIVPILCSFDHSMVDG
                     ESFPNESRRIQSFCRALKTCIEEFPGTCVIASADLSHLGPLYGDPEGLDEKGILSLEQ
                     GDNEILQSIESGSASGFAEAIRKNDNATRICGFPPIYTMLSVLGDQRGLVLDYGGTQM
                     DEHGSVVTFASVVFSPDLKASGDV"
     gene            complement(2378..>2786)
                     /locus_tag="PKH07_08245"
     CDS             complement(2378..>2786)
                     /locus_tag="PKH07_08245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34972.1"
                     /translation="ALLVATLCLIVLFMMDYYGSYASYSAQDWDYGWRDALARLQAER
                     TTHPNAVISGWTSYPDVFIRFYMGLLPNPPGDLPPKGFSFLPFKSDASHYLERFPRPA
                     VFLGRPGELQGLPVLETIRYPDGSPAWNIIEID"
BASE COUNT          739 a          717 c          706 g          624 t
ORIGIN      
        1 gacggaagcc tcaaacaact tcttcagagc agggattcaa ggacgggaca atggctcgcc
       61 tctccgatga agcatcccat gagaggcaag agacgtccgg ttgacaatgt ccactggctt
      121 gaagtaatcg gtgctcatgc aaacaacctc aagaacgtag atgtccgatt tcctctgcag
      181 agactttctg ttgtgactgg agtgtctggt tccggcaaga gcagcctcgt gcgagatgtt
      241 ctgtcccgtg cctgcagagc caagctcaat aataaacgaa tacctaagaa ttgctgcaaa
      301 aatctccttg gctggaagtc cctttctcgt acgatggaag ttgaccagac acctatcggg
      361 cggacatctc gatcctgtgt agcaacctat gtcggtctta tggacgatat ccggaatctc
      421 ttcgctatga cttcagaggc gcggatgctg aactacggag ccggtcgttt ctctttcaat
      481 gtccccggtg gccggtgtga gaagtgtctt ggacagggca gcatagatgt cgagatgagc
      541 ttcttaccaa atgttgccgt tccctgtgag gaatgtgaag gaaagcagta caacgaagat
      601 acgctcaaag tgcacttcaa aggcaagaac atcgctcaag ttctctcaat gtccgttgaa
      661 gaagcgctgg agttccttgc ggggttttcc tctctgacag agcctctctc tttccttcgt
      721 gatatcggtc ttggctatct gacacttggg caaacaagcc cgacgctttc cggtggagaa
      781 gcccaaagga tcaaactgac atctgaactc tcaaggagat ccagaactcc ctgctgctac
      841 ttcatcgagg aacctacaac aggacttcac gggagcgacg tgatcaagct tctgaatgtt
      901 ctccatgctc ttgtagacaa gggacacact ctcgtggtga ttgagcatca tctcgatgtg
      961 atcgcagaag cggactacgt tgtggatctc ggacctgaag ccggtcaaga gggaggctgg
     1021 gttgtcgcca gtggaatccc tgaagagatc gccgagattg agcactccca tacagcacgt
     1081 ttcctctcca aagtactcaa gcacaagccg aagcgcatta cacgtcccca gatgccttca
     1141 ggtctgggga aaagaccact gaggcaaaag tgacgaccga gccgtgctca tccatctgcg
     1201 tcccaccata atccaggacg agacctcgct gatcacccag tacactgagc atcgtgtaga
     1261 ttgggggaaa accacaaatc cgggtggcat tgtcgttttt tcgaatagct tctgcaaatc
     1321 cactggcact cccagattct atggactgaa gaatctcatt gtcaccttgc tcgagtgaga
     1381 gaattccctt ttcgtccaga ccttcagggt caccatagag cggcccaaga tggctgagat
     1441 cagcgctggc aataacacac gtccctggaa actcctcgat gcatgtcttg agagcacggc
     1501 agaacgactg gattctccga gactcattcg ggaacgattc cccatcaacc atagagtggt
     1561 caaaggaaca gaggatcgga acaatcttga cgcgactgga aaaggagtgc tgaagaaacg
     1621 taacctgaaa ctcaatgacg tgttcagcac gatgggcaaa ctcgtcactg aaaaggctat
     1681 cgccgaagac ttctgaaagc ctgtcaagga aggcttgatc agtcgctgaa atacccaacg
     1741 gtgtctcgaa gtccttcctg gttgctgcaa aaagattctc cagaccgcgg tgtgctgttc
     1801 ccaatatgac gactctctct ggagactgcg tggctgacga aaggcatttg aaggcgctgg
     1861 catagcaagg cccaccagca cgaatgtcaa tgtgaggcac gacaagacct gtgatgtgag
     1921 cccgtgatga aagccttctc gagtcctgtc ggtctcggat gttctcaaaa taggtgtcca
     1981 gttgcctctt caatgcctct ggttccgact cataggaaag ccctgcatga gtagctttcc
     2041 gaattgtctc atttgagaaa tccctcattg cattctcgcg gtgaactgca aaacgctcag
     2101 tgtcaagaag gtaatgttca tccaggctcg caatgatctc tgctacctgt tcggaagtga
     2161 ccatctcgcc cgttgtgcgc atgaactcag cctgaatgtc tcttatcgag tgattcccat
     2221 ccatgaaagg aaacaagtag aatacaggaa ccggaacggc aataatatga tccacaaagc
     2281 ccgtcgcatc cctaacacac acaacgtctt tgccggagac ttgcgcctga aacgcttcaa
     2341 caggcctcag ttttgggaaa tcagtcatcg gaaaacctca atctatctcg atgatattcc
     2401 aagcgggcga accgtctgga taacgtatag tctcaaggac aggcaacccc tgcaactcac
     2461 caggcctgcc caggaagaca gcgggtcttg gaaaacgctc caagtagtga gaagcatcag
     2521 acttgaatgg aagaaagctg aaacccttgg gcggcagatc accgggtgga ttgggtagaa
     2581 gacccatgta gaaacggatg aacacatcgg gatagctagt ccaaccagag ataacagcat
     2641 tcggatgtgt tgttcgctcg gcttgaagtc tggccaaggc gtctctccag ccatagtccc
     2701 aatcctgagc ggaatatgat gcgtaagagc cgtagtaatc catcatgaag agaacgatca
     2761 aacagagtgt tgcaaccagc agagcg
//
LOCUS       DAOQUC010000425         2786 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_42888_length_2786_cov_2.360308, whole genome shotgun sequence.
ACCESSION   DAOQUC010000425 DAOQUC010000000
VERSION     DAOQUC010000425.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2786
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42888_length_2786_cov_2.360308"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="PKH07_08250"
     CDS             <1..271
                     /locus_tag="PKH07_08250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34973.1"
                     /translation="LGNVPLNHMTATAVVEMNVQGTVAGVMIAPGMVAGVDVRREAVV
                     TVSQTDVALEEDSIEPGVGRRGDFPFPPEVLISCDAYWNQKTSPL"
     gene            237..692
                     /gene="dut"
                     /locus_tag="PKH07_08255"
     CDS             237..692
                     /gene="dut"
                     /locus_tag="PKH07_08255"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012963809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HNT34974.1"
                     /translation="MRIGIKRLPHSEGLPLPQYMTAGSSGIDLLAAVQGEVVLRPGEI
                     QAIPTGLIMAIPTGFEGQVRPRSGLALRYGISLPNAPGTVDSDYRGEVKVILINLGKE
                     DFVIQRGDRIAQMVFCHVEQAQLNELTELECTDRNDGGFGHTGHSVQDG"
     gene            780..1220
                     /locus_tag="PKH07_08260"
     CDS             780..1220
                     /locus_tag="PKH07_08260"
                     /inference="COORDINATES: protein motif:HMM:NF014078.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF116 domain-containing protein"
                     /protein_id="HNT34975.1"
                     /translation="MLDRFIIFLNKAIGKLRTSRAKPSEILVLLPHCIQNKDCDLDLT
                     SGVQICRRCGRCKIREVSLLQEEYGFRSAVVGGGQQAVQATKDKGVKCVVAIACEKEL
                     AAGILSVLRKPVHTIRNSQPFGPCVNTDMDADSLREMLQKIVKR"
     gene            1424..>2786
                     /locus_tag="PKH07_08265"
     CDS             1424..>2786
                     /locus_tag="PKH07_08265"
                     /inference="COORDINATES: protein motif:HMM:NF025182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4185 domain-containing protein"
                     /protein_id="HNT34976.1"
                     /translation="MDTYSTRSSPIGHLFFLLLAAIVAYLLPVLVLADTPLYFAGTTR
                     KILQVTGDTDQPLRRNTLPMTRTNAGVIATDLGSSFEHNGRLCFLFGDTFGTNGTQDR
                     DCLAFSDATVPEDLTLDFHLGQDGVFLPIAIPSVSQGAYEVPSYGISVGGNIYIVHTT
                     NWYSTNSNMERSVMARSNDDGLTWTRLYTLSSAQSHDMSQAHFINVSMAKIAAEDYPG
                     ELPYATGEIVLIWGSGAYRKSNVYLACIPSAQIDDRSALRYLSGYTAGGVPLWSTEES
                     ESLALFEHPCVGEFSTAWIAPLQRWLILYNGSLPRGITMRSALKPWGPFSSGTVILDP
                     WADSAYGKYLHVPWNWVRMDGFHDSGRPNEWGGEYGPYLIPRFTRGTARECQIYYTLS
                     TWNPYQTILVRSTVGQPLPFTSANEKIYLMPGDETWNTTSPAFFVPLTVAGRAYISTW
                     TSEQ"
BASE COUNT          621 a          721 c          765 g          679 t
ORIGIN      
        1 tctggggaac gtccccctga atcacatgac cgcgacagcg gtggtggaga tgaacgttca
       61 agggacggtg gcaggggtta tgatcgcccc aggaatggtg gcgggggtgg acgtccgcag
      121 agaggcggtc gtgaccgtca gtcagaccga cgtggccctc gaagaggata gcatagaacc
      181 tggtgttggg aggagagggg acttcccctt tcctcccgag gttctcatct cttgtgatgc
      241 gtattggaat caaaagactt ccccactctg aaggcctccc ccttccgcag tatatgactg
      301 cagggtcctc gggaattgat ctccttgcgg cggtgcaggg agaagtggta ctcaggccag
      361 gagaaattca ggctatccca accggactga taatggcaat tccaactggc tttgaaggac
      421 aggttcgtcc tcgaagcggt ctcgcgcttc gctacgggat ttcacttcca aatgctcccg
      481 gaaccgttga ttctgactat cgtggggagg tcaaagtcat tctgataaac ctcggcaaag
      541 aggatttcgt catccaaagg ggcgatcgga ttgcacagat ggttttctgt cacgtggaac
      601 aagcccagtt gaatgagctg acagaactgg agtgcacgga ccggaatgac ggtggattcg
      661 gtcacacggg acatagtgtg caagacggct gattgacaat atgttttccc aattccacaa
      721 tctgcccgaa agaggttttc gagagttcag cagagactct aatcagggac ggctaaggga
      781 tgcttgaccg attcatcatt tttctgaata aagcgattgg caaactgcgt acaagccgtg
      841 cgaagccctc ggagatcctt gtgctcctcc cgcattgcat ccagaacaaa gactgtgatc
      901 tcgatctgac gagtggtgtt caaatctgcc gtagatgtgg acgctgcaag attcgggaag
      961 tgagtctgct tcaagaagag tatggcttca gaagtgctgt tgttggcggt gggcagcagg
     1021 cggtgcaggc caccaaggac aagggcgtga agtgcgttgt tgccattgca tgtgaaaagg
     1081 aacttgcagc aggaatcctc tccgttcttc ggaagcccgt ccatactatc cgtaacagtc
     1141 agcccttcgg gccttgtgtc aataccgaca tggacgcaga ttccttgcgc gagatgcttc
     1201 agaagattgt gaagcgctga aaaccagact gttccgcggt gcgatcgcgt gtcatccttt
     1261 ttgtgggttg acagagacgc cgtccagtgg catggtttcc atgcacgtcc ggaactgacg
     1321 gatctgccag ggcttctgac ctgccctcat tttcagctag tgcctgctcc ctatttggag
     1381 cgggtcttct catcccactt cgaaaggtct gagagaacat actatggata cttacagcac
     1441 gcgatcctca cctatcggac acctgttctt tttgcttctc gctgccattg tggcatatct
     1501 gcttcccgtc ttggtcttgg cagatacacc gttatatttc gccggcacaa ctcggaagat
     1561 cttgcaggtc accggtgaca cggatcagcc tctgcgacga aacactctgc cgatgacccg
     1621 aaccaatgct ggggtgattg cgactgactt ggggagctct ttcgagcaca acggaagact
     1681 gtgctttctc ttcggcgaca cgtttggtac caacggtaca caagacaggg actgccttgc
     1741 cttttctgac gcgacggtgc ccgaagacct gacactggat ttccatctcg gacaggatgg
     1801 ggtttttctt ccaatcgcga tcccaagtgt ttctcaaggt gcatacgaag ttccttcgta
     1861 cggaatatcc gttggaggaa acatctacat cgttcatacg accaactggt acagcacgaa
     1921 ctcgaacatg gagcgttctg ttatggctcg ttcgaatgac gatggactga cttggacgcg
     1981 gctctacacg ctgtcctcgg ctcagagtca cgacatgagc caagcgcatt tcatcaacgt
     2041 gagcatggca aagatcgcgg ctgaggacta tcccggtgaa ctcccctatg ccactggaga
     2101 aattgtcctc atctggggca gcggtgccta ccgcaagagc aacgtatatc ttgcctgtat
     2161 tccatcagct cagattgatg accgttccgc actccgatat ctctcaggat atacggctgg
     2221 cggagttcct ctatggagta ctgaggagtc ggaaagcctc gcgctgtttg aacatccctg
     2281 tgtgggggag ttttccactg cgtggatcgc tcctctacaa cgctggctga tactgtataa
     2341 tggaagtctg cctcgtggta tcacgatgcg atctgctctg aaaccatggg gacccttttc
     2401 gagtggaacg gtcatccttg acccatgggc tgattcggcc tatggcaagt atcttcatgt
     2461 gccgtggaat tgggttcgaa tggatggttt ccacgattcc ggacgcccta atgaatgggg
     2521 aggcgaatac ggtccctatc tgatcccaag gttcaccaga ggaacggctc gggagtgtca
     2581 gatatactac acgctctcca cttggaatcc ctatcagacg atcctggttc gatctacggt
     2641 tggccaacct ttgcccttta cttccgccaa cgagaagata tacttgatgc cgggagacga
     2701 gacttggaac accacctcgc cagcgttctt tgttccactc acagttgctg gcagagccta
     2761 tatttcaact tggacctctg agcagg
//
LOCUS       DAOQUC010000426         2785 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_42910_length_2785_cov_3.052747, whole genome shotgun sequence.
ACCESSION   DAOQUC010000426 DAOQUC010000000
VERSION     DAOQUC010000426.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2785)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2785)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2785
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42910_length_2785_cov_3.052747"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..531
                     /locus_tag="PKH07_08270"
     CDS             <1..531
                     /locus_tag="PKH07_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNT34977.1"
                     /translation="NRQRAGDSDAFSGGQRQRIGIARALALNPRLIVCDEPVSALDVS
                     VQAQIINLLKHLQREFGIAYLFIAHDLSVVENISRRIGVMYAGRIVEFAETTELFSHP
                     LHPYTEALMSALPQIDRRARGKRIILPGEVADPSDLPTGCAFHPRCPKAQACCSSEVP
                     QLRLISEGHFSACLFS"
     gene            complement(640..2511)
                     /locus_tag="PKH07_08275"
     CDS             complement(640..2511)
                     /locus_tag="PKH07_08275"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNT34978.1"
                     /translation="MARRLAVRGLIFGLFSCCALCAMAFQEAPMLAERVEQGLLAPVQ
                     ERLPEEPLVVEPIESIGRYGGIWRRVVVGPFDMMLGARMGYEPVVRWDRTGKHVVPGL
                     AQSWEIEDEGRTYVLHLRRGLRWSDGVEFTSEAFRFAYEEFLLNKELVPVFPGWLTID
                     GTPAVIQTPDKHTVVFHFEKPYGIFLELLAFRGGQGAVYLPSHYLKQFHINHADKSAI
                     QELLKEAGFELWYQLFARRASLNENPDLPTMGPFQLKTKPPAQRVIAERNPFYWKVDP
                     VGNQLPYIDQVAFAQVQNIESANFKALTGDVDYQARYMNPANYSLFMQNRAKGGYRVQ
                     ADVNPCSLVIYVNQCSKDLQLRPLLQDRRFRIALSVAINRKELIDLVYSGMAEPARGV
                     ASPFDPYYLPEYDSKYLEYDPELANRLLDELGLKRTSAGMRRMPDGKPFRQILNIYPS
                     EMGTSFDLWELVADYWREVGLDFVTKTDAKTLSVLQVQNGNSDFWAYSIAGLHWVIDP
                     LWYIPWYKSSYFAPLFGQYQDSGGKVGEKPLPEYQRLIDWYLELRSVVGDDALKLELG
                     RKILGQWAEECYTIGICRDQLITIVSNRFRNVPKEIIHDYRVLTPGYIGIEQFYMEQ"
     gene            complement(2581..>2785)
                     /locus_tag="PKH07_08280"
     CDS             complement(2581..>2785)
                     /locus_tag="PKH07_08280"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNT34979.1"
                     /translation="SGVDAFPILREKRPQMKILICSGFELDSVSQRLIEAGAGGFVQK
                     PFELHTLAREIRAILDKKTDARS"
BASE COUNT          684 a          786 c          685 g          630 t
ORIGIN      
        1 aatcgccagc gcgcgggcga ttccgatgcg ttcagcggtg gccagcgtca gcgcatcggg
       61 atcgcccgcg cgctggcgct taacccaaga ttgattgtct gtgatgaacc cgtctcagcc
      121 cttgatgtat cggtacaggc gcagatcatc aatttgctca agcacctcca gcgagagttc
      181 ggaatcgcct atctgtttat cgcccatgat ctttctgtcg ttgagaacat ttcacggcgg
      241 ataggcgtca tgtatgcggg gcgaattgtg gagtttgcgg aaacaacaga gctgttttcg
      301 catccacttc atccctacac agaggcactc atgagcgccc ttccccagat tgaccgccgc
      361 gccagaggca aacgaatcat tctgcccgga gaagtcgccg atccatccga cttgccaacg
      421 ggctgcgcct tccacccacg gtgtccgaaa gcacaggctt gttgctcgag tgaggttccc
      481 caactgcggc tgatcagcga agggcacttc tctgcttgtc tcttctccta ggaaagcaga
      541 cttcggacga ggctgtttct ctcgcgagca ggccccgcac aggactgggc aaagtccatg
      601 tgcctgcagg cgttagtctg ctcatgaaaa accaagcgcc tactgttcca tgtagaattg
      661 ctcgatgccg atgtacccag gagtcagaac gcggtaatcg tggatgattt ccttggggac
      721 attccgaaaa cgattggaga caatcgtgat aagctgatcg cgacagatgc cgatggtgta
      781 acattcctca gcccattgac ccagaatctt cctgcccaat tccaatttca gggcatcgtc
      841 gccgaccact gaacgcagtt cgagatacca gtcaatgagg cgctgatatt ccggcagcgg
      901 tttctcccca acctttcctc cgctgtcctg atactgcccg aacagcggtg caaaataact
      961 ggatttgtac caggggatgt accaaagtgg atcgatcacc caatgtagac cggcaatgct
     1021 gtaagcccag aaatcactat ttccgttctg cacttgaagc acactcagtg tcttggcgtc
     1081 tgtcttggtc acaaaatcca gcccaacctc ccgccaatag tctgccacca attcccacag
     1141 atcgaagctc gtgcccatct cggaaggata tatattcaga atctgcctga aaggcttgcc
     1201 gtcaggcatt cgacgcatcc ccgccgaagt tcgtttgagc cccaactcat ccaacaagcg
     1261 attggccagt tccggatcgt actccagata cttcgagtca tattcaggca ggtaataagg
     1321 atcgaatggt gaagccactc cgcgcgctgg ttccgccatt ccggaataga caagatcaat
     1381 gagctccttt cggttgatcg ccaccgaaag tgcgatgcgg aaacggcgat cctgcaacaa
     1441 cggccgcagt tgcaagtctt tgctgcattg attcacataa atgaccagcg aacagggatt
     1501 gacgtccgcc tgaaccctat acccaccctt ggcacggttc tgcatgaaaa gcgagtagtt
     1561 ggccggattc atatatcgcg cctggtagtc cacatccccc gtcagcgctt tgaaattggc
     1621 tgattcgata ttttgaacct gtgcaaatgc aacttggtca atgtaaggca actgattccc
     1681 caccggatcc actttccagt agaaggggtt tcgttctgcg atgactctct gggctggcgg
     1741 tttcgtcttc agctgaaagg gccccatggt tggaagatcg gggttttcat tgagtgatgc
     1801 gcgtcgcgca aaaagttgat accacaattc aaaacccgcc tctttgagca actcctgaat
     1861 tgcagatttg tcggcgtggt tgatgtgaaa ttgtttcaga tagtgagaag ggagatagac
     1921 cgccccttgt ccgcctcgaa acgccagcag ctccaggaag atgccgtaag gcttctcaaa
     1981 gtgaaagacg acggtatgtt tgtcaggagt ctgaatgacg gccggagttc catcaatggt
     2041 caaccaacct gggaagacag gaacgagttc tttattaaga aggaattcct cataggcgaa
     2101 acgaaatgcc tcagatgtaa actcaactcc atccgaccat ctgagtcctc tgcgaagatg
     2161 aagaacatag gttctgccct catcctcgat ctcccaagac tgcgccagac caggaacgac
     2221 atgcttccct gtcctatccc agcgaacgac cggctcatat cccatcctcg cacccaacat
     2281 catgtcaaag ggaccaacaa caacgcggcg ccagattcca ccgtatcggc cgatactctc
     2341 gatgggctca acgacaagag gttcctcggg aagtcgctcc tggacgggtg caagaagacc
     2401 ctgttcaacg cgctcagcaa gcatgggtgc ttcctgaaat gccatggcac acaacgcaca
     2461 gcaagaaaaa agaccaaaga taagtccgcg tactgccagt cgtcttgcca ttggacgctc
     2521 caatatgaac tccatggaga tcaaagttca aaatagctcc atggggctcc gtctagaatg
     2581 tcaactcctt gcatcagttt tcttgtcaag aatcgctcga atctctcgag caagggtgtg
     2641 cagttcgaaa ggtttctgaa caaaacctcc ggctcccgct tcgatcagcc gctgggaaac
     2701 ggaatccagt tcgaatccac tgcagatcag tatcttcatc tgaggccgct tctcgcgaag
     2761 gatgggaaat gcatcaaccc cactc
//
LOCUS       DAOQUC010000427         2777 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_43081_length_2777_cov_3.170096, whole genome shotgun sequence.
ACCESSION   DAOQUC010000427 DAOQUC010000000
VERSION     DAOQUC010000427.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2777)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2777)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2777
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43081_length_2777_cov_3.170096"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            171..1430
                     /locus_tag="PKH07_08285"
     CDS             171..1430
                     /locus_tag="PKH07_08285"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNT34980.1"
                     /translation="MRILHLLSDSRFTGPAQPVLQLVMELRRRGHDAQIGCNRRPPTW
                     RISKKLHGEGHFLFLARRLKVEPVGDFEREIRNVQVPLVEQLDLNRYFNIRDNIRSLF
                     ALRDLQRGEKYDIIHLHLPHDHWIGAVAKWIFRSDANLVATIHKVEKPHSGLFHKLLY
                     TRMIDRIAVVSERSRQMVLRHLRVHSRNVRTIWGAVDVEKFHPGITGEKIRAELGIQP
                     DALVIGMVARFQPHRQHAILVRAADRVLERFPHARFLLSGRGEYKEEIEKLGYQRGLE
                     QKVLFAGYRKEDYPETIAATDLLFFSVPGSDGTCRAVLETMALGKPVVAFDTGVLPET
                     IEDEHTGWITPTGDQLALEETLMKALRNPDQIRRMGEAARETVCRRHTVSGQADIIEE
                     FYSDLLEEKALAAGTRLAHRAGSMPQA"
     gene            complement(1414..>2777)
                     /locus_tag="PKH07_08290"
     CDS             complement(1414..>2777)
                     /locus_tag="PKH07_08290"
                     /inference="COORDINATES: protein motif:HMM:NF028476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34981.1"
                     /translation="CRKRLLEIFSMMRSDGWFPRQYSVAGVGGTHDLREYVDSGWWVW
                     ELFYEYLCQTRDFSVLNEAVGYLDSDAKSTVIEHACQLLGYALHKDNLGEHGLLKIRE
                     GDWNDSVNRAGVLGRGESVMASAQVVLALTQAVETFSYLLKTKVITHSLKRSLEPLIK
                     KYKTAMTRMIRALLIHARNQQGYFNGVFNDAGKWCFSPCDPDGRSRINGPVNSFAVIA
                     GIVGGAEREQVFEALNSLKTDVGWLLFSPAIGNPPIEHLGRIGSGDLAEGLGENGTPY
                     NHGSHGFLGRACWTAGKGNMLRDVLCSMLPYDQRAHPVGRSLTAPYAVVNHWRTAPGM
                     TGEGGDTFLSGSISTALRNIWHGLVGFRPDVEGLVLDPCIPTDWKEVHATVSHLGKTF
                     RMAIHNPNGVSCGVHTVRVGKDLYKPNRVFKQGGRKVICIPAEDLEALSDKSIDVIVT
                     LGA"
BASE COUNT          632 a          776 c          738 g          631 t
ORIGIN      
        1 attcgcatgt acttctttca gcgtggattc cttgacggca cacccggctt gattgtgtgc
       61 atgctgggaa gcctcagcgt atttgcgcgt tatgcaaggc tgtgggaaat gctcgagatt
      121 cgctcaaagc agaatgcgaa cgacagccag agccccgatc ggagaacgag atgaggattc
      181 tccatctgct gagtgattcc cgtttcacgg gccctgcaca gccggtactg caactggtca
      241 tggagctgag acgccgcggg catgacgccc agatcggctg caatcgccgt cctcccacat
      301 ggcgaatctc caagaaactt cacggagagg gtcattttct ctttctggca cgccgattga
      361 aggtggaacc tgtgggggat ttcgagagag agattcgcaa tgtgcaggtg ccgttggttg
      421 agcaactgga tttgaaccgt tatttcaaca ttcgggacaa catccgaagt ctgtttgctc
      481 tgagggatct gcagcgcggc gagaagtatg acatcattca tcttcacctg cctcacgatc
      541 actggatcgg cgctgtcgcc aagtggatct tccgaagtga cgcgaatctg gtcgccacaa
      601 tccacaaagt tgagaagccc cacagtggcc tttttcacaa attgctttac accaggatga
      661 ttgaccgaat cgctgttgtg tctgaacgaa gtcgccagat ggtcttgcgt catctgcgcg
      721 ttcattctag aaatgtgcgc acaatttggg gtgcggtgga tgtcgaaaag ttccacccgg
      781 gaatcacggg agagaaaatc cgcgcagaac tcggcatcca acctgacgcc ctggtgatcg
      841 gtatggtggc gcggtttcag ccacatcgcc aacacgcgat tcttgtgcga gcggcagacc
      901 gcgttctgga gaggtttcct catgctcggt ttcttctgag cggccgaggt gagtacaaag
      961 aagagatcga gaagctgggc tatcagcggg ggttggagca gaaggtactc tttgccggtt
     1021 accgtaagga agactacccg gagaccattg ccgccacgga tctgctcttc ttctctgtgc
     1081 ctggttcaga tggaacctgt cgtgcagtgc ttgagactat ggcactcggc aaaccggtgg
     1141 ttgcgttcga cacaggagtt ctcccggaga ctatcgagga cgagcacacc ggctggatca
     1201 ccccaacggg ggatcaactt gccttggaag aaaccctcat gaaagccctt aggaatcccg
     1261 atcagattcg acgtatgggc gaggctgcgc gcgaaacggt ctgccggcgt catactgttt
     1321 ccggtcaggc ggacatcatc gaggaattct actcagattt gcttgaggag aaggctctgg
     1381 cagccggtac gcgtctcgca catagggcgg gttctatgcc ccaagcgtaa cgatcacatc
     1441 aatgctcttg tctgatagcg cctccaaatc ctccgccgga atgcagatca cctttcttcc
     1501 accctgcttg aagaccctgt tgggcttgta gagatctttg ccgactcgta cagtgtgcac
     1561 accacacgaa acaccgttgg gattgtggat ggccattctg aacgtctttc ccaaatggct
     1621 gacggttgca tgaacttcct tccaatcggt cggaatgcac gggtcgagca ccaagccctc
     1681 aacatccgga cgaaagccga ctagaccatg ccagatgtta cgcagtgcgg tgctgatcga
     1741 tccggagaga aaagtatctc ctccttctcc tgtcatccca ggtgctgttc gccaatgatt
     1801 cacaacagca tagggagccg tcagtgatct gccaacggga tgcgccctct gatcgtaagg
     1861 aagcatagag cacagaacat cccgcagcat gttccccttc cccgcagtcc agcacgcacg
     1921 cccgagaaaa ccatggctgc cgtggttata tggcgtaccg ttttctccaa gtccttccgc
     1981 cagatctccc gacccaatac gacccaagtg ttcgatgggc ggattcccaa tggcgggaga
     2041 gaacaacaac cagccgacat ctgtcttcaa cgagttgaga gcctcgaata cctgctctcg
     2101 ctcagctcca cctacgatgc ccgcaatgac tgcaaagctg ttgacgggtc cgttgattct
     2161 cgatctgccg tctggatcac acggagagaa acaccatttt ccggcatcgt tgaaaactcc
     2221 gttgaagtaa ccttgctggt ttcgagcatg aataaggagc gctcggatca ttcgcgtcat
     2281 tgctgtcttg tactttttga tcaagggctc cagagaacgc ttgaggctat gggtgatgac
     2341 cttcgtcttg agaagatatg agaacgtctc caccgcctgg gtaagcgcca aaacaacctg
     2401 cgcgcttgcc atgacagatt caccacgccc gagaactccc gcacggttga cactgtcgtt
     2461 ccagtcgcct tcgcggattt tgagtaagcc gtgttctcca agattgtcct tgtgcagagc
     2521 gtagccgagc agctgacagg catgctcgat gacagtgctc tttgcatcac tatcgagata
     2581 accgacggcc tcattcaata ccgaaaagtc tcgcgtctgg cagagatact cgtagaacaa
     2641 ttcccaaacc caccagccac tgtctacgta ctctcgcaga tcgtgcgtgc caccaacacc
     2701 cgcgacgctg tactgtcttg gaaaccagcc gtcactgcgc atcatggaga atatctccaa
     2761 cagccttttg cgacacg
//
LOCUS       DAOQUC010000428         2776 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_43115_length_2776_cov_3.004410, whole genome shotgun sequence.
ACCESSION   DAOQUC010000428 DAOQUC010000000
VERSION     DAOQUC010000428.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2776)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2776)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2776
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43115_length_2776_cov_3.004410"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..726)
                     /gene="rseP"
                     /locus_tag="PKH07_08295"
     CDS             complement(<1..726)
                     /gene="rseP"
                     /locus_tag="PKH07_08295"
                     /inference="COORDINATES: protein motif:HMM:TIGR00054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HNT34982.1"
                     /translation="MGFDALSVLIVLGLAILFHELGHFVVAKLLGVRVLRFSIGFGPK
                     LFGVKRGHTEYILSAIPMGGYVKMAGDEWGQEHEGGPWEFLAQTPMRRNLIVLAGPVM
                     NVVLAYVIYFLIFAFWGQFFVATTEVGVVDMGSPAAIAGIRPGHRVSSINGKKMESWD
                     DVMASFAATPLDKYEIEVVSETGTYSTTVKFDSSEEKDPAGSLPPVIGSLSPGSVAKS
                     MGIEPGSTVLSVNDEPVACWEELR"
     gene            943..1728
                     /locus_tag="PKH07_08300"
     CDS             943..1728
                     /locus_tag="PKH07_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019534367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HNT34983.1"
                     /translation="MPVKRKVGVIVESFRKQVREGIHLASQLKLDGIQVYVTHGEMAP
                     ENLSKSGREEFLAFVRSQGLELSALCGDLGHGFVDPNKNQELIEKTKKMVDLCLDLST
                     PILSTHIGVIPEGAQHPVWQVLTEALEEIGAYAENRDCVLATETGPESTELMARFLGS
                     LKTSAIKVNYDPANLAMNEFDPVKGVRDLGQRIVHTHAKDGLGPKNGGPKEVPLGEGD
                     VPFKDYLAALDSIGFDGFLTIEREVGDDPVADITKARDFLRSL"
     gene            complement(2054..>2776)
                     /locus_tag="PKH07_08305"
     CDS             complement(2054..>2776)
                     /locus_tag="PKH07_08305"
                     /inference="COORDINATES: protein motif:HMM:NF014714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HNT34984.1"
                     /translation="RIRKMKRLVDLMLVILPFEEQLYQEAGVPVHFVGHPLLDQIPDQ
                     LLQETVRQTNLIGLLPGSREGEVRRILPVMRDAALLLRKSLPAIELFLPLSPNVPRSL
                     VEEILGNIEVNIVPSPAYELRHQAGFALTASGTATLENALLGLPMAIIYKLMPLTYLI
                     GRLLVQVDRIGLVNIVAQEDVVPEFIQSRARPEDISRYVLEYLQNPSHQKTVLGALQK
                     VQKSLGEPGASERAAGLVLGKE"
BASE COUNT          758 a          718 c          662 g          638 t
ORIGIN      
        1 acgaagttcc tcccaacagg caacaggctc gtcattgact gacagaacgg tgcttccagg
       61 ttcgatgccc atagactttg ccacgctgcc gggactcaga ctacctatca cagggggaag
      121 ggaaccggcc ggatccttct cttctgaaga gtcaaacttc acagttgtgc tgtatgtccc
      181 cgtctctgag accacttcaa tctcatactt atccaagggt gttgcggcga aactcgccat
      241 cacatcatcc catgattcca tcttctttcc gttgatgctg gagacgcgat gccccgggcg
      301 tattccagca atagccgccg gactacccat atcaacaaca cccacttctg tagtcgcaac
      361 aaaaaactgt ccccaaaatg caaaaataag gaaatagatg acgtaagcca gaacaacatt
      421 catcaccggg ccggccagga cgatcaaatt cctccgcatc ggtgtctgag cgagaaactc
      481 ccaaggtccc ccttcgtgct cctgccccca ttcatcacct gccattttga catatccacc
      541 cattgggata gcagaaagga tatactccgt gtggcctctc ttgacgccaa aaagcttagg
      601 accaaagcca attgagaatc gtaatacccg gactcctaag agtttagcaa caacaaaatg
      661 acctaattca tgaaataaaa tggctaaacc caaaacaatt aaaacactta gcgcatcaaa
      721 acccatgttc caaaccttca aacaaggata tagttgacaa taggcaaaga actctaatgc
      781 atccttctcc gactgtcaaa aactttctct acaatctgac aacttcaaac gaaacagaaa
      841 ccatcaggca gaaacctctt ttcgcttgaa gactgtcctt agaacaacta acatcccaaa
      901 agtgaaaata ttcattccaa acatctaatc aggaggctat tcatgccagt gaagagaaaa
      961 gttggagtta tcgttgaatc cttccgtaag caggttagag aaggaataca tctggcctca
     1021 cagctgaaac tcgatggtat tcaggtctat gtcacccacg gtgaaatggc tccggagaat
     1081 ctctcaaagt cgggaagaga agagttcctt gcctttgtaa gatcacaggg gcttgagctg
     1141 agtgcgctct gcggggattt gggccatggc tttgttgatc cgaataagaa tcaagagctt
     1201 atcgaaaaga ctaagaagat ggtagacctc tgtctggact tgagcacacc tattctctcc
     1261 actcatattg gtgtgattcc agagggtgct cagcatcccg tatggcaagt cctcaccgag
     1321 gcacttgagg agatcggagc ctatgcggag aacagggatt gtgtactcgc gaccgagacc
     1381 ggccctgaaa gtactgagtt gatggcaaga ttccttggat cactcaagac gtcagccatc
     1441 aaggtcaact acgatccggc caatctcgcg atgaacgaat tcgatcctgt caagggagtg
     1501 agagatcttg ggcagcgcat cgtccataca cacgccaaag atggactggg acccaagaat
     1561 ggcggaccaa aggaagtccc tctgggcgag ggcgatgttc cattcaaaga ctaccttgca
     1621 gcactagaca gcatcggctt tgatggattc ctcacgatcg aaagagaagt gggcgatgat
     1681 cctgtcgcag atatcaccaa ggccagagat ttcctgcgta gtctctaacc gccgcaggca
     1741 actgtaacgg ctcaattatg atacgcgaca tcatcggtct gctcttgatg gtctcacttg
     1801 tggccgcaat cctgacagtc gcctgcagac gcctcactgg caagcgaggg agatcgaaat
     1861 acgattgccc gacgtgtgtg catgatttgg agaactcatg ccgcattccc gacagaccat
     1921 acatggagaa ctgtagagcc tacaggagag tgtcggggaa gcacggctga gaagcgacag
     1981 atttgatgtt acttaagact aaaagaaagt ttacataaga tatcttatgc gacattttgt
     2041 ccttggtgtc ctcttactct tttccgagca caaggcctgc tgccctctct gaagcacccg
     2101 gttccccaag cgacttctga actttttgca gtgccccaag aacggtcttt tgatgtgatg
     2161 ggttttgaag atattcaaga acgtagcgag agatgtcctc gggtctcgct ctgctctgga
     2221 tgaactcggg aacgacgtct tcctgggcga caatgttcac gagtccaatc ctgtcaactt
     2281 gaacaaggag acgcccgatg agataggtca gcggcatcaa cttgtagatg attgccattg
     2341 gaagtccaag gagggcgttc tcaagcgtgg cagttcccga tgcagtgagt gcaaagcctg
     2401 cctggtgcct caattcgtac gcgggactcg gaacgatgtt gacctcaatg tttccgagaa
     2461 tctcctccac gaggcttctg ggaacattgg gtgacagcgg aaggaagagt tcaatggccg
     2521 gcagactctt tctgagcaga agtgccgcat cccgcatcac gggcagaatc cgtcggactt
     2581 ctccttccct gcttccggga agaaggccaa tcaggttggt ctgcctgact gtctcctgca
     2641 gcaattggtc gggtatctga tccaacagcg ggtggccaac aaaatgaaca ggaactcctg
     2701 cttcctgata taattgttcc tcaaatggta atatgacgag catcagatca acaagacgct
     2761 tcatctttcg gatccg
//
LOCUS       DAOQUC010000429         2773 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_43174_length_2773_cov_3.434879, whole genome shotgun sequence.
ACCESSION   DAOQUC010000429 DAOQUC010000000
VERSION     DAOQUC010000429.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2773
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43174_length_2773_cov_3.434879"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2051
                     /locus_tag="PKH07_08310"
     CDS             <1..2051
                     /locus_tag="PKH07_08310"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HNT34985.1"
                     /translation="GDKKPSRYWDLFDVLAGANGAKEVFLLTATPVNNSLHDFRHMIE
                     LFSRQKEDYFKDTLGIHYLRGHFVNLDKKLQATRPKSEVDSLGETNLVEAEQVLTTDA
                     IFDALVVQRSRAYVQKSQVQQGFSSTLFPVREHPQVAPYSVKKTYGRLLDMVEKAFKK
                     EKPLFVLGIYYPLAYYKGPDVSVDPMLENRQKQVVSLIRTQFLKRFESSAKSFELSCA
                     RLMKKLLAWLTKYAETDHEKRRLDRFLAKEGDLIGHVQKSAKDLWDLNEEEAEEDFIT
                     EEMLEAVEDLPPNEYKVDEMIDDCFDDLEQIAEFLRELQQFQAKHDDKLNALVKLLKK
                     DSILSKHKVLVFTEFADTAHYLKQQLKEAGIDGVFQIDSGTKGDRSSILERFAPYYNG
                     TSSAEIEARGEKEIRVLISTDVLSEGLNLQDATRLINYDLHWNPVRLMQRIGRVDRRM
                     NPAVEKQMLEDHPEMEKVRGTVAYWNFLPPDDLEELLKLYNRVSNKTLVISKTFGIEG
                     GKLLHPDDDLEGLKEFNQEYEGETSPTEELLLEYQGHLKDHPELAERLSKFPLRVFSG
                     KQHPKKDSRAAFFCYRIPHMDREMLDEEGEPTWTEEAGETRWYLYNLEDCLILESSQA
                     IADFIRCQLQTPRHCVTPQETLSEVRRKIEKHIKNTVLKQLQAPLWVKPLLKAWMELS
                     "
     gene            2251..>2773
                     /locus_tag="PKH07_08315"
     CDS             2251..>2773
                     /locus_tag="PKH07_08315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34986.1"
                     /translation="MGTTHRDQLRDIRTFPSLMKYLRDELHWPIGSESFDDLTFDYEP
                     EELGIDSEVAAKIDSIKQLRPLSSNQPWGIFFVKFEPKRLPVVALRRVLRSLVIRKRS
                     SAARANQAAWNLHDLLFISAYGEEAERHITFAHFTEDPNSGDLPALRVLGWDDADTAL
                     HLDHVHQELSDKLK"
BASE COUNT          685 a          683 c          763 g          642 t
ORIGIN      
        1 tgggagataa gaagccgtcg cgctactggg acctcttcga tgttcttgca ggcgccaacg
       61 gagcaaagga agtctttctc ttgactgcga ccccagtgaa caactccctg catgatttcc
      121 ggcacatgat tgaactgttt tcgcgacaga aagaggacta tttcaaggac actctgggca
      181 ttcattatct acgaggacat ttcgtaaatc tggacaagaa gcttcaggcg acacgcccca
      241 aatctgaggt ggattcattg ggggagacca atcttgttga agccgagcaa gttcttacaa
      301 cggatgctat cttcgacgcc ctggttgtgc agcgcagccg tgcttatgtt cagaagagcc
      361 aggttcagca aggcttcagt tcgacgctct tcccagttcg ggaacacccc caagttgccc
      421 cttactcggt caagaagacg tacggaagac tcctggacat ggtcgagaag gctttcaaga
      481 aagagaagcc gcttttcgtc ctgggcatct actacccact ggcttattac aaggggccag
      541 atgtttccgt cgatccgatg ttggagaacc gacagaagca ggtggtcagc ctgattcgaa
      601 cgcagtttct caaacggttt gagagttcgg caaagagttt tgaactctcc tgtgcgcgtt
      661 tgatgaaaaa acttcttgcc tggctgacaa aatatgcaga gacagatcat gagaagcgcc
      721 gactcgatag attccttgca aaggaaggtg atctgatcgg ccatgttcag aaatcagcaa
      781 aggacctgtg ggaccttaac gaagaagaag ccgaggagga tttcatcacc gaagagatgt
      841 tggaagccgt tgaagattta cccccaaacg aatacaaagt tgatgagatg atcgacgact
      901 gctttgacga tctggagcag attgccgagt tcttgcgtga actgcagcag tttcaggcga
      961 agcacgatga caagttgaat gctctggtca agttgctcaa gaaggactcc attctcagca
     1021 aacacaaagt attggtattc accgaattcg ccgacacggc ccactatctc aaacagcaac
     1081 tcaaagaggc cgggatcgat ggcgttttcc agatcgatag cggcaccaag ggcgaccgaa
     1141 gttccattct tgagcgattc gccccctatt acaacggaac ctccagtgcg gaaattgagg
     1201 cgcggggaga gaaggaaatc cgcgttctca tctccacgga cgttctttcg gaaggtttga
     1261 accttcagga cgcgaccagg cttatcaact acgatttgca ctggaatccc gttcgtttga
     1321 tgcagagaat cggtcgtgtg gatcgccgta tgaacccggc tgttgaaaag cagatgcttg
     1381 aggatcatcc ggagatggag aaggtccgag gaaccgttgc ttattggaac ttcctgcctc
     1441 ccgacgatct tgaggaactg ctcaaactct acaatcgcgt ctcaaataag acgttggtca
     1501 tctccaagac ctttggcatt gaagggggta aacttctgca tcccgatgac gacttggagg
     1561 gtctgaagga gttcaatcag gaatacgaag gcgagacttc cccgacggaa gagttgcttc
     1621 tggagtatca ggggcacctg aaggatcatc cggagttggc tgaaaggctt tccaagtttc
     1681 cactacgagt cttctctggc aagcaacatc cgaagaagga ttcccgtgcg gctttctttt
     1741 gctaccggat tccccacatg gaccgggaga tgttggacga ggaaggcgaa ccgacctgga
     1801 cggaagaagc tggagagaca cgctggtatc tctataacct tgaggactgc ctcattctgg
     1861 agagttcgca agccattgcg gatttcatcc gttgtcagct acagactcct cgccattgcg
     1921 tcacgcccca ggagactctt tctgaggtgc gcagaaagat tgaaaagcat atcaaaaaca
     1981 ctgtccttaa gcagttgcag gctcccctct gggttaagcc tttgctgaaa gcgtggatgg
     2041 agctgagtta gcgcggaggt tgaggatgcc ggaaaacggg acgtattgtg gtgcaggctt
     2101 ccagcctgcc tctgcggacc gttgtggtgc gggcttccag cctgcctctg cggaccgttg
     2161 tgatgcaggc ttccagcctg catccgatcc ggaagaagaa gacgcaggca ggatgcctgc
     2221 accgcaacta taggagggga aggaaaaact atgggcacga cgcacagaga tcagcttcgg
     2281 gatatacgga cttttccgtc cctcatgaaa taccttcggg atgaactgca ctggcccatt
     2341 ggatcggaga gtttcgacga tctgactttt gactatgagc ctgaggagtt gggtatcgac
     2401 tccgaagtgg cggcgaagat cgactccata aagcaactgc gtccgttgag ttccaatcag
     2461 ccgtggggaa tctttttcgt caagttcgag ccgaagaggc ttccggtggt tgcgttgagg
     2521 cgtgttcttc gttcccttgt catccggaag cgttcttccg cggcaagagc gaatcaagcg
     2581 gcttggaatc ttcacgattt gcttttcatc tccgcatacg gcgaggaagc ggagcgccac
     2641 attacctttg cccatttcac tgaagacccg aactcgggtg atctgcctgc cttgagagtt
     2701 ttgggctggg atgatgcaga cacggcactt catctggacc acgtgcatca ggagctttct
     2761 gacaagctca agt
//
LOCUS       DAOQUC010000430         2772 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_43200_length_2772_cov_3.201325, whole genome shotgun sequence.
ACCESSION   DAOQUC010000430 DAOQUC010000000
VERSION     DAOQUC010000430.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2772)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2772)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2772
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43200_length_2772_cov_3.201325"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..586)
                     /locus_tag="PKH07_08320"
     CDS             complement(<1..586)
                     /locus_tag="PKH07_08320"
                     /inference="COORDINATES: protein motif:HMM:NF014766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HNT34987.1"
                     /translation="MALSFEKFQLVLKEPFGISRGVSSVRDSLFVTMDGCVGEAVPSL
                     YNGETIETVISLMKRFEDQIPAEPEGVIHFMQQVNRQIKGNPTFKAALSMLLWDRLGK
                     DLGAPLYKIFGLSPSKAPQTSFTVGISTKEDMLRKVQDAAEFPILKIKLGFPKDIEVM
                     EAIREITDKVLRVDANGGWTAAEAIGKVDRLAELG"
     gene            complement(626..1456)
                     /locus_tag="PKH07_08325"
     CDS             complement(626..1456)
                     /locus_tag="PKH07_08325"
                     /inference="COORDINATES: protein motif:HMM:NF016816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HNT34988.1"
                     /translation="MKVFMSADIEGVSGVASDQRQAESSGMDYQRARRLMALDVNAAI
                     EGLMMAGAKEIVVNDSHGGMTNLWPEDIHPEAQLILGGRKPLSMMQGIDASYDAAVFV
                     GYHARAGSRYGTLAHTYSGQIFELRLNGKPAGETQVNGALAGYFGVPLILVTGDQELA
                     QQVKESNPAIETAVVKYAHTRGATQCLHPEKGRAIIREAAQRALQRKQDIAPLKYEMH
                     VRFEVTFYATQMADIVERVPGTERVDNRTVSYVAQKDYLEGFKAFLAIMSLSAGAKDV
                     "
     gene            complement(1453..1749)
                     /locus_tag="PKH07_08330"
     CDS             complement(1453..1749)
                     /locus_tag="PKH07_08330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNT34989.1"
                     /translation="MCRLRFGMKVEDVDVTKALRRLNNTAVLIIQSEHDPIVSRQQTE
                     RILASLGQFGAYWFVPNAQHVRGFEEQPDEYKRRVLGFFADSLKEARQQRGERS"
     gene            complement(1765..2355)
                     /locus_tag="PKH07_08335"
     CDS             complement(1765..2355)
                     /locus_tag="PKH07_08335"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNT34990.1"
                     /translation="MLVLTSGLFSAVCVILGVAYYSSKQITIPCRGCPQGDPGDLGLP
                     FENVQFTTEDGLTLEGWYIPAKSSESSSQRVVIVCHGQMAIRWDCLDYVPFLREAGYG
                     VLLFDFRAHGTSEGDICTIGFLERLDVKAAVQFMLHHSQPEWIGILGLSMGGSAAILA
                     AVDLPAINAVVADCPFATFTDAIFDVCRKFRLPVLG"
BASE COUNT          634 a          812 c          674 g          652 t
ORIGIN      
        1 cgccgagttc tgcaagccga tcaactttcc cgatggcttc cgccgcagtc cagcctccgt
       61 tggcatccac acgaagaacc ttgtccgtaa tctctcgaat ggcttccatg acttcaatgt
      121 ctttgggaaa accaagcttg attttcagga tgggaaactc cgcggcatcc tgaaccttgc
      181 gcagcatatc ttctttggtg gaaatgccga cggtgaagga cgtttgcggc gccttcgacg
      241 gcgacaagcc gaatatctta tacagtggcg cgcccaaatc ctttccaagg cgatcccaga
      301 gcaacatgct cagagcagcc ttgaatgtcg gattgccttt gatctgtctg ttgacctgct
      361 gcatgaaatg aatgacgcct tccggctccg ctggaatctg gtcttcgaaa cgcttcatca
      421 gcgagatgac cgtttcaatt gtctccccgt tatagagact cggcacggct tccccaacac
      481 agccgtccat ggtgacaaaa aggctgtccc gaacgctgga cacacctctc gaaatgccaa
      541 aaggctcctt caaaaccagc tgaaatttct caaacgatag tgccattttg cttcctccag
      601 gaatcgttaa tgcgttcttc gctgctcaaa catccttcgc tccggccgac aaagacatga
      661 ttgccagaaa ggccttgaac ccttcaagat agtccttctg cgccacataa ctgacggtgc
      721 gattgtccac tcgctctgtt ccgggcactc gctcgacaat gtccgccatc tgtgtcgcat
      781 agaacgtcac ctcaaatcga acgtgcatct catatttcag aggcgcaatg tcttgtttcc
      841 tctgcagggc tcgttgggca gcctcccgaa tgatcgcccg tcctttttcg ggatgaaggc
      901 attgcgtggc gccacgtgtg tgggcatatt tcacaacggc cgtctcgatg gccggattgc
      961 tttccttgac ttgttgcgcc aactcctgat cccctgtcac cagaatcagc ggaacaccaa
     1021 agtatcctgc aagagcaccg ttgacctgtg tctctccagc gggtttcccg ttcagtcgaa
     1081 gttcgaagat ctgccccgag taggtgtgtg ccagcgttcc atatcgtgat ccagcgcggg
     1141 catggtaacc aacgaaaacg gctgcgtcat aactggcatc aatgccctgc atcatggaca
     1201 ggggtttgcg accacccaga atcaactgag cctcagggtg aatgtcctca ggccagagat
     1261 tcgtcatccc gccatgcgag tcattgacga cgatttcttt ggcacccgcc atcataagcc
     1321 cttcgatggc agcattcacg tccagtgcca tgagtcgtcg ggcacgctgg tagtccattc
     1381 cagatgactc tgcctgcctt tggtcagatg ccactcccga gaccccttca atatccgcgg
     1441 acataaagac tttcaactcc gttctcctct ctgttgtctt gcttctttga gactgtctgc
     1501 aaaaaagccg agcacccgac gcttgtactc atcgggctgt tcctcaaaac ctcgaacatg
     1561 ctgggcattc ggaacaaacc agtatgctcc aaactgcccc agcgatgcaa gaattcgttc
     1621 agtttgctgc cgcgagacga tgggatcatg ctcactctgg ataatcaaca ccgccgtgtt
     1681 gttcaagcgt ctgagggctt tggtcacatc cacatcttcc accttcattc caaaacgcag
     1741 ccgacacatc tggatgccca tccatcaacc gagaacaggc agtcggaatt tgcgacagac
     1801 atcgaaaatg gcgtctgtga atgtcgcgaa cggacagtcg gcgacaaccg cattgatggc
     1861 aggtagatcg actgcggcca gaattgctgc ggatcccccc atcgagagac caaggatccc
     1921 gatccattcg ggttggctat ggtgcaacat gaactgcact gctgccttga catcaagccg
     1981 ctccagaaaa ccgatggtac agatgtcgcc ctcactggtt ccatgtgccc ggaagtcaaa
     2041 tagcagcacg ccgtagcccg cttcacgcag gaagggaaca taatcaagac agtcccagcg
     2101 aatggccatc tgaccgtgac agacgatcac cacccgctgt gatgatgatt cggacgattt
     2161 ggctggaatg taccaccctt ccaaggtcaa accgtcctcc gtggtgaatt ggacgttttc
     2221 aaacgggaga ccaagatcgc cgggatcacc ctgcgggcag cccctgcatg gaattgtgat
     2281 ctgcttggaa gaataatagg caacacccaa gatcacgcat actgccgaga acagtcctga
     2341 tgtgagaaca agcatctgag ccctttcagg caatggactt accgaacgca tatattgcca
     2401 tgggacaaca agagagcata tcggaaactt ggattccgtc aagcgcagga tactgcatca
     2461 cttccctcgc cacttgcccg ttgtcgctgc gtgatgccga gtgccctttc tttccagttt
     2521 ggcagccttc aactcggagg ctgtctcaaa accctcttgg ggttgtcatg ctgagcgaag
     2581 cgaagcatct ctctgatcgc tgaaagcccc atagaatcga gattcttcac tttgctttgc
     2641 tccgttcaga atgacagaag gtgtcgctct gatgagtttt gagacagcct cctcgttgag
     2701 ggctgcttct cgatgtgaca caggcattcg tgccgatgat cctctgccct gggatcgccg
     2761 agcgtctgct cg
//
LOCUS       DAOQUC010000431         2770 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_43233_length_2770_cov_3.729282, whole genome shotgun sequence.
ACCESSION   DAOQUC010000431 DAOQUC010000000
VERSION     DAOQUC010000431.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2770
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43233_length_2770_cov_3.729282"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..508
                     /locus_tag="PKH07_08340"
     CDS             <1..508
                     /locus_tag="PKH07_08340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34991.1"
                     /translation="IKIANIPSPLDVGEGRILFSGGYNAGAMMIRLTRRADAIEPEIL
                     FRTDAKTFGADQHTPIYYEGLIYGIIPGGKLACLTPEGQRLWVDEENNFGLGPFMMVD
                     EKLLVLDDDQKKPGGLSVFRVGSEGATKIATTSVLDGHEAWAPMAFASGKLVLRDVTT
                     MVCLDLNW"
     gene            505..1131
                     /locus_tag="PKH07_08345"
     CDS             505..1131
                     /locus_tag="PKH07_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005866845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HNT34992.1"
                     /translation="MNTVELERLRGTMMNENKHKHLTRREFMDRTTRGVTAFVLGGVA
                     GKVLFNGIKHERVWQIDPFKCVQCGKCATHCVLTHSASRCVHAYEVCGYCRICSGFFP
                     TDVAVLDEGAENQICPVGAIKRTFVEEPYYEYTVDRDLCIGCARCVKGCTAHGNGSLY
                     MQIQRDLCVNCNECSIALVCEGKAISRIAADVQYLKKGFVDNSEQVST"
     gene            1312..>2770
                     /locus_tag="PKH07_08350"
     CDS             1312..>2770
                     /locus_tag="PKH07_08350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34993.1"
                     /translation="MLEVNYETGRMSCGQRNTVFSDRPYLYSLRGRVGSSEMMGAELT
                     QRAVLSKRTETIITGLFRGLGVALRQRFCVCGSGIQETITLKNLNAEPVLLSDIGLGF
                     AAEIGGRSEWRLFAIPFLVQLDGNRHDYTTSDLTGGTFRNAVYSDETRPFIPLAEEGR
                     LRSEAWGWWHKDHGLVVIKYNDEDIELSVAQPLVIGDEQILRFGGVGTCLYGEPSGGH
                     RLQPDGKVTPKCCQWDESTGHCALAPESEFTFGTTYYLPVEGTIEDALVCYRDFLDSR
                     GHCFPTDYDPPVNWNELYDVGWYHSDAEKLSQNYTRERLLKEAEKASDCGCELLYLDP
                     GWEQAEGTTLWDEERLGTVGSLIEVLKKDYGLQLGFRTILRCYRDYWPHRHMVQHPGR
                     PKEPINWPGQLLWELCLCDHSLWEQKLERISKIAQEGVRFLMVDEMDWRGPCHDPTHG
                     HAVPTTASDHARAVYGLCRELRRLCPGLAIECHDPV"
BASE COUNT          619 a          661 c          828 g          662 t
ORIGIN      
        1 gatcaagatt gccaatatac cctctcctct ggatgttgga gaggggagga ttcttttcag
       61 tggaggctat aacgctggtg ctatgatgat ccgactgacc aggcgtgccg acgccataga
      121 gccggagatt ctcttcagga cagacgcaaa gacttttggt gccgatcagc ataccccgat
      181 ctactacgaa ggcctgatct atgggatcat cccaggaggc aaactcgcct gtctgactcc
      241 ggaaggacag cgtctgtggg ttgatgagga gaacaacttc ggcttaggcc ccttcatgat
      301 ggttgacgaa aagttgctcg tgctcgatga tgatcagaaa aagcctggtg gactgtctgt
      361 ttttcgggtc ggttcagagg gagccacgaa aatcgctaca acctcagtgc ttgatggcca
      421 tgaagcgtgg gcacccatgg cttttgcatc gggcaaactg gttcttcgag acgtgacaac
      481 gatggtgtgt ctggatttga actggtgaat actgtggagt tggaaagact cagaggtacc
      541 atgatgaatg aaaacaaaca caaacacctg actcgcaggg agtttatgga cagaaccacg
      601 cgaggggtga cggcttttgt attgggcggt gttgcgggca aggttttgtt caacgggatc
      661 aaacatgaga gagtctggca gatcgatcct ttcaagtgtg tccagtgtgg caaatgcgcc
      721 acgcattgtg ttctgacgca ttccgcttcc cggtgtgttc acgcgtatga ggtatgtggc
      781 tattgccgca tctgctcagg tttctttccc accgatgttg ctgttttgga cgaaggtgct
      841 gagaaccaga tctgtcctgt tggtgctata aagaggacat ttgtcgaaga gccctattac
      901 gagtacacgg ttgatcgaga cttgtgcatc ggctgtgctc gctgtgtaaa aggatgcact
      961 gcgcacggta acggttctct ttacatgcag atccagagag atctgtgcgt caactgcaat
     1021 gaatgttcca ttgcgttggt ttgtgaaggc aaggcgatca gccggattgc ggcggacgtg
     1081 cagtatctca agaaaggctt tgttgacaat tccgagcagg ttagcacctg aaggcgtgtg
     1141 ggctatgccc actccttcgg acgcttgctc aatgaatgat aagcgccttt cggactttgt
     1201 gtcactagga gttccgaggg atgaggggat tcgcattctc ttaagaggct ttgatccaat
     1261 ttgctgatca gtggtttccc gttcgacaga aggcgggagg aggcatcacg catgcttgaa
     1321 gtaaactatg aaacaggacg tatgtcgtgt gggcaacgga atacggtatt ctcggataga
     1381 ccttatcttt actcactgcg cggccgtgtc gggagctcgg aaatgatggg tgccgagttg
     1441 acccaaagag ccgttttgtc gaaacgtact gagacaatca ttactggact ctttcgcgga
     1501 ctcggcgtcg ctttgcgaca gaggttctgc gtttgcggtt caggtatcca agaaaccatc
     1561 acgctcaaga accttaacgc agagccagtt ttgttgtcgg acattggtct tggttttgcc
     1621 gctgagatcg gaggacgatc ggaatggcgt ctgtttgcca ttccttttct ggtgcaactg
     1681 gatggcaaca ggcacgatta caccacatcg gacttgacgg gaggaacatt ccgaaatgcg
     1741 gtctattcag acgagactcg ccctttcatc cctctcgccg aggaaggccg tttgcgctcc
     1801 gaggcctggg gttggtggca caaagaccat gggctggttg tcatcaagta taacgatgag
     1861 gatatcgagc tgtcggtggc gcagcctctt gtgattggcg atgagcagat tctcagattt
     1921 gggggtgttg gcacttgtct gtatggtgaa cccagcggtg gacaccgtct gcagccagac
     1981 ggcaaggtga cgcccaagtg ttgtcagtgg gatgaatcaa ccggacactg cgcgcttgcg
     2041 ccagagagcg aattcacatt tggcacgact tattatcttc ctgtcgaagg caccattgag
     2101 gacgcgcttg tctgttacag agatttcctt gattcacgcg gacactgctt tcccaccgat
     2161 tatgatccac cggtcaactg gaatgagctc tacgatgtcg gatggtatca ttccgatgcc
     2221 gagaaactga gccagaacta cacccgagag aggctactca aagaggccga gaaggccagt
     2281 gactgtggct gtgagctgct ctatcttgat ccaggatggg agcaggcgga aggaacgacc
     2341 ctgtgggatg aggagcgtct cggaacggtg gggagtctga tcgaggtgct caagaaagac
     2401 tacggtttgc agctcggatt tcgcacgatc ctgcgctgct atcgggacta ttggccacat
     2461 cgccacatgg ttcagcatcc tggaaggccc aaagagccga tcaactggcc tgggcaactc
     2521 ctctgggaac tctgcctctg tgaccattca ctctgggagc agaaactgga gcggatcagc
     2581 aagatcgcac aagaaggggt tcggtttctg atggtggacg agatggactg gcgcgggccc
     2641 tgccacgacc cgacccatgg gcacgccgtt ccgacaacag cctcggacca cgcgcgcgcc
     2701 gtgtacgggc tgtgccggga gctgaggcgc ctgtgccccg ggctagcgat cgagtgccac
     2761 gatccagtgt
//
LOCUS       DAOQUC010000432         2770 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_43242_length_2770_cov_2.800368, whole genome shotgun sequence.
ACCESSION   DAOQUC010000432 DAOQUC010000000
VERSION     DAOQUC010000432.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2770
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43242_length_2770_cov_2.800368"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="PKH07_08355"
     CDS             complement(<1..182)
                     /locus_tag="PKH07_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003351999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT34994.1"
                     /translation="MVTHPKEPWQALSVLVVGFGSIGRRHLRILQELGLGNLSVVDPR
                     PDRRETACRDFGITEV"
     gene            complement(176..991)
                     /locus_tag="PKH07_08360"
     CDS             complement(176..991)
                     /locus_tag="PKH07_08360"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNT34995.1"
                     /translation="MAENQTVQELFDLTDKAAFVTGGARNLGFDMALALAESGANVTI
                     TSRNLEQCKKSAAQINKTTGRRVLPLALDVCREDQVERAIEAHISEFQRLDILVNNAG
                     NVLSTPETAPLEKRSLEDWHAVIEVNLTGVFLCTKHALAKAMMPQRSGSIINIASVTG
                     MIGKDRRVYRGTTMGGATIDYHAAKGGVISMTRDMACYLAPFNIRVNCISPGGFWRNQ
                     PEAFVKAYSETIPMGRMGQDGKEMKGAVVFFASEASSYCTGQNLAIDGGLTAW"
     gene            complement(1024..2358)
                     /locus_tag="PKH07_08365"
     CDS             complement(1024..2358)
                     /locus_tag="PKH07_08365"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HNT34996.1"
                     /translation="MTSPAQVQQSSFDKVEPQPTATEWLRTKPDLVVYVPDNPESPDN
                     TNQHFNVVATPEGDFLATWTTATMESCPDQRVVLSRSTDRGRTWSEPEVLDGPEPEDP
                     PGTGLASWQFLIVAPEIAPAGKTRIWCFYNKNVGVDDARTADTGTLRGRYSDDGGQTW
                     SANYHEYPIAPNSISNPDPSIPSTWIVYQNPVVTKERHVLAGFTRWASNAVDPGMGMF
                     ERHSEICFLRFENILQEPDPERLIVTTWPRAPHGICVPNPNRPGRSTAQEPTAQTLSD
                     GRLISVFRTMQGRIFYSLSEDDGRSWDAPRVLRYSPGGEELRNPLAPCPLYKLCDGRF
                     LLVFFNNDGSVHGGLGPTDSVNVRNPAFVTVGREIQGETDHPIRFGSPKVFADTDWTP
                     PYPGGKHTQVASYPSLLEDRGERILFYPDRKHFLLGKYLTDEWLDECDPGSL"
     gene            complement(2530..>2770)
                     /locus_tag="PKH07_08370"
     CDS             complement(2530..>2770)
                     /locus_tag="PKH07_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HNT34997.1"
                     /translation="RVFLVYGYRHQPFGIRARILNSECSDFKTAEEIVLRDDGGSTDL
                     GYPWAAMLPDGRILAVYYFNLSNGTRQIAGTFITL"
BASE COUNT          639 a          773 c          704 g          654 t
ORIGIN      
        1 aagacttcgg tgatgccgaa atctctgcag gctgtctcgc gcctgtctgg tcgagggtct
       61 acgacactca ggtttcccag gccgagttcc tgcaagatgc gcaggtgcct ccgaccgatg
      121 gaaccaaagc ccaccaccaa gacgctcaga gcctgccacg gttcttttgg atgggttacc
      181 atgcagtgag ccctccatca atggcaaggt tctggcctgt gcagtaactt gatgcctcag
      241 aggcaaaaaa gaccacggca cctttcattt ccttgccatc ctgtcccata cgtcccatag
      301 ggattgtctc gctgtaagcc tttacaaaag cttcaggctg attacgccag aagccacctg
      361 gactgataca attgacccga atgttgaagg gagcaagata gcacgccatg tcacgagtca
      421 tactgatgac gccacccttg gcagcgtggt agtcaatcgt ggctcctccc atggtggttc
      481 ctctatacac gcgcctgtcc ttgccaatca tccccgtcac tgaggcgatg ttgatgatgg
      541 aaccgctgcg ctgtggcatc atggcctttg ccaatgcgtg ttttgtgcaa agaaagacgc
      601 cggtcagatt cacctcaatg acagcatgcc agtcctcaag actgcgtttc tcgagtggtg
      661 cggtttccgg tgtgctgagc acattgcccg cattgttgac aaggatgtca agtctttgga
      721 actcgctgat gtgagcttcg atggcacgct cgacttgatc ctcccgacac acatccaacg
      781 ccaaaggcag aacacgcctt cccgtggttt tgttaatctg cgctgcagac ttcttgcact
      841 gttcgaggtt ccgtgacgta attgttacgt tggcacccga ttcggccagc gccagtgcca
      901 tgtcaaagcc gagattcctc gctccccccg taacaaaggc agccttgtcc gtgaggtcaa
      961 acagttcttg gactgtttgg ttttcagcca ttttccctac tctctccttc ttcatccgca
     1021 gtctcataga ctgccaggat cacattcatc aagccattca tcggtcagat actttcccaa
     1081 aagaaaatgt ttacgatcag ggtagaacaa gatgcgttca cctctgtcct caagcagact
     1141 tggataactg gcgacttgcg tgtgtttgcc accaggatac ggtggagtcc agtctgtatc
     1201 agcaaaaacc ttgggcgatc caaaccgaat tgggtgatca gtctcacctt gaatctcacg
     1261 tccaaccgtc acaaaagcgg gattgcgcac attgacactg tccgttgggc caaggccacc
     1321 gtgaacgctt ccatcattgt tgaaaaaaac caacagaaac cgcccatcgc agagtttgta
     1381 cagtggacag ggtgcaagcg ggtttctcaa ctcctctcca ccagggctgt aacgcagcac
     1441 ccgaggcgca tcccaacttc ggccatcgtc ctcggaaagg gaatagaaaa tcctgccctg
     1501 catcgtgcga aacacagaaa tgagtcgtcc gtcagaaagg gtttgagccg tcggttcctg
     1561 cgccgtgctt ctgcccggac ggttaggatt tggaacgcag atcccgtgag gcgctctcgg
     1621 ccaagtggtt acaatgagcc tctcgggatc aggctcttgc aggatattct caaatcgcag
     1681 aaaacagatc tcagaatgcc tctcaaacat ccccattccc ggatcaacgg cattgctcgc
     1741 ccagcgagtg aagccagcca ggacatgcct ctctttcgtg acaactggat tctggtagac
     1801 aatccaggtt gagggaatgg aaggatcggg attgctgatg gaattggggg cgatgggata
     1861 ctcgtgataa ttggcactcc aagtctgtcc accatcatcc gagtaacgtc cacgcagtgt
     1921 gccggtatct gccgttcggg catcgtccac accaacattc ttgttgtaga aacaccagat
     1981 tcttgtcttg ccagccggtg cgatctccgg agccacaatc aagaactgcc aactggcaag
     2041 ccctgttcca ggtggatctt ccggctcggg accgtcgagc acttcaggtt cgctccaagt
     2101 acgtcctcgg tcggttgatc ggctgagaac aaccctttga tcgggacaac tttccatcgt
     2161 cgcagtagtc caggtggcca aaaagtcgcc ttctggcgtg gccaccacat tgaagtgttg
     2221 attcgtgttg tcaggagact ctggattgtc aggcacatag accaccagat caggttttgt
     2281 cctcagccat tcggtcgctg tcggctgggg ttccacctta tcgaagctgc tctgttgaac
     2341 ctgtgcagga ctcgtcattc tacctcagtc aagacccaaa agggcctatt gccgcgctca
     2401 atgttgtaag aacgtctgaa caactataca acttctatca gaggatgagg acgaaatcaa
     2461 gcagaaacat tcgatgatac gcattctcag ccaaggcggc gttttcagcg ttgggcggat
     2521 cttgccgttc tacagcgtga tgaaagtccc ggcgatttgt ctggtgccgt tgctcaggtt
     2581 gaaatagtag acagcgagaa ttcgtccgtc agggagcatc gcggcccagg gataacccaa
     2641 gtctgtgctt ccaccatcat ctcggagcac gatctcctca gcagtcttga agtcggagca
     2701 ttctgaattg agaatccggg cgcggatgcc aaagggctga tgacggtatc catagaccag
     2761 aaacacccga
//
LOCUS       DAOQUC010000433         2769 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_43265_length_2769_cov_2.539794, whole genome shotgun sequence.
ACCESSION   DAOQUC010000433 DAOQUC010000000
VERSION     DAOQUC010000433.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2769)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2769)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2769
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43265_length_2769_cov_2.539794"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..739)
                     /locus_tag="PKH07_08375"
     CDS             complement(<1..739)
                     /locus_tag="PKH07_08375"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNT34998.1"
                     /translation="MVESGFKECPYCFEVIREKAKVCRFCHAVLTEEPIEPVFVTPSK
                     AALEHERALEDLPSSQLTREEILNKEQENGIRQILKNAPDEYRMATVLFADLCGYTRV
                     SSEVSAEEMKGYLDAFYDQSIHAVSVYNGFVVKFVGDGILAVFGAPVAYDRDAESAVR
                     AALEIRDQVSAMPSINGKHLRVKVGIHTGEILSTVVTARGRQATFDIFGDAVNIACRL
                     QSVAKDGQILVSGQTFDLVRHAFELGNK"
     gene            complement(767..955)
                     /locus_tag="PKH07_08380"
     CDS             complement(767..955)
                     /locus_tag="PKH07_08380"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNT34999.1"
                     /translation="MMEEKLLTLDDLAEYFQVSKKTIYRLVQQKELPAVRISHQWRFR
                     KQDVEGWIQEKMVEDQSA"
     gene            1146..2102
                     /locus_tag="PKH07_08385"
     CDS             1146..2102
                     /locus_tag="PKH07_08385"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNT35000.1"
                     /translation="MTHRERAITALKRGQPDHVPTFELVFHETEADFQGRTFFGAPGQ
                     PDPTGTTYEDQLRHNAQLYVDLARHFDHDILFLSSGTPPAAKDHLQCLLDTAQAIRDI
                     AGDEYLLMAHGDATYGVPSGQHMMDFVMWLADKPDEAKDQAKRSVDNMLEASRKMLEG
                     GIEGFIQCSDYAFNSGPFLSPAMFAEFVTPFLKELTAQQRKMGAYVIKHTDGNIMPII
                     DQLVDCEPHALHSLDPMAGVDIKYVKETYGDRIALCGNVHCAHLQTGTREEIAESAEY
                     CLKWAKPGGGYIFCTSNCVFRGMPIESYHLIHSIWMKHRDYL"
     gene            2489..>2769
                     /locus_tag="PKH07_08390"
     CDS             2489..>2769
                     /locus_tag="PKH07_08390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35001.1"
                     /translation="MPANAPDESPVQAQPSGISPQGERSDVSPRQARKSDGESPTQPN
                     VAFSQDDDVYDVSERHFSSESSPLGAILEVCRMLTRALDLEEVLPRALQV"
BASE COUNT          660 a          758 c          708 g          643 t
ORIGIN      
        1 gtttgttccc caactcaaaa gcgtgcctga ccagatcaaa ggtctgaccc gagacgagga
       61 tttgcccatc tttggcaacc gactgcaggc gacaggcgat gttcaccgca tcaccaaaaa
      121 tgtcaaaagt ggcttgtctg ccacgtgccg tcaccactgt ggagagaatc tcgccagtgt
      181 gaatgccgac tttgacgcga agatgcttgc cattgatgga cggcatggcg gagacctgat
      241 cgcgaatctc caatgccgca cgtaccgcag actccgcgtc acggtcatag gcaactggag
      301 cgccgaaaac tgccagaatg ccatctccga cgaacttcac aacaaatccg ttatacacag
      361 acacggcgtg gatggattga tcatagaaag cgtcaaggta ccccttcatc tcctcagccg
      421 aaacttcgga agaaacgcgg gtatagccac aaagatcagc aaagagcacc gtcgccattc
      481 ggtactcatc aggtgcgttt ttcagaatct ggcgaatgcc gttttcctgt tctttgttga
      541 ggatctcttc acgggtcagc tgacttgacg gaaggtcttc aagtgcccgt tcgtgctcca
      601 aggctgcttt ggacggggta acaaaaacgg gctcaatcgg ctcctcggtc agtacggcgt
      661 gacagaaccg acagactttg gccttctccc ttatcacttc gaagcaataa gggcattcct
      721 tgaaacctga ttcgaccacc tgtttcccct ccctctcgtg cggagatcaa gcggactgat
      781 cttcaaccat cttttcctgt atccagcctt ccacatcctg cttgcggaag cgccactggt
      841 gcgaaatgcg caccgcaggc aattcctttt gctggaccaa acggtagatg gtcttcttgc
      901 tgacctggaa atactctgcc aggtcgtcaa gtgtcagaag tttctcttcc atcattttgt
      961 cctcttttgt ctactgttgt ccagttctga gtatatttat acatcattgg aatctcttgt
     1021 caactatttt ctctgaatct gacctgttgc ctttttgaaa ccgaaaagcg atgagaattc
     1081 ctgaaaaagc gtgttacaat gcactctgat actaacactg ctgaatgagg agagacacgg
     1141 actcaatgac ccaccgagaa agagcaatca ccgccctgaa acgagggcag cccgaccacg
     1201 ttccaacgtt tgagctggtc tttcacgaga cggaagccga tttccaggga cgcactttct
     1261 ttggtgcacc cgggcagccg gacccgacgg gaacgaccta tgaggatcaa cttcgccaca
     1321 atgcccagct gtacgttgac ctggcgcgac actttgacca tgacatcctg tttctgtctt
     1381 cgggaactcc cccagctgca aaggaccatc tgcagtgcct cttggacaca gcccaggcga
     1441 ttcgtgacat tgcgggggac gaatatcttc tcatggcaca cggtgatgcc acttacggag
     1501 tcccatccgg tcaacacatg atggatttcg tgatgtggct cgcggacaaa cctgatgagg
     1561 ccaaggatca agccaagcgc agcgtggaca atatgctcga ggcctcccgc aagatgcttg
     1621 aaggtggaat cgagggattc atccagtgct cagactacgc cttcaactcg ggtccgttcc
     1681 tgtcgcccgc aatgttcgcg gaattcgtca cgcctttcct gaaggaactg accgcccaac
     1741 agcgcaaaat gggcgcctac gttatcaagc ataccgatgg caatatcatg ccgatcatcg
     1801 atcagctggt ggactgcgaa ccgcatgccc tgcattcatt ggatcccatg gcaggcgttg
     1861 atatcaagta cgtcaaggaa acgtatgggg atcgaattgc cctgtgcgga aatgttcact
     1921 gcgcccatct tcagacaggc acgagagaag agattgctga gagtgcagag tactgcctca
     1981 agtgggcaaa acccggtggc ggatacatct tctgcacgtc aaattgcgtt tttcgcggta
     2041 tgcctatcga gagttatcac ctcatccatt ccatctggat gaagcatcgg gactatctat
     2101 agcacggcag tctgttacat tcgtgtctgt acagacatca tgggcacaga ggttccccct
     2161 ctgagtgcgt tggcatcttg cctcgactct gtcaccgtaa cgatgagtcg ccagacccaa
     2221 ccgcacccgg atgacccatc gagacagaca ttgtcccttt gagactgcac aaaaaccttg
     2281 acagtctctt ctgagacagc tagcttgtca tggaaagttg gaacgtatga gggacttgcg
     2341 ccttggtagg ggcgaagtgg aggaaatgcg ttttgagccg gagggatgac tgcgttatag
     2401 cagcctggcc gacggaaggt gaacttgaag acaacggcct tatcttaaga agttgcctgc
     2461 gaccgcctct gtgaggaggg aacgactgat gcctgcaaat gctccggatg aatcccctgt
     2521 tcaggcgcaa ccgtctggca tttctccaca gggagagcgt agcgacgtat cgccgcgaca
     2581 agctcgtaag agcgatggag agtctccaac gcagccaaat gtcgctttct ctcaagatga
     2641 tgatgtctac gacgtcagtg agcgacattt ctcatccgag tcttctcctc tcggcgcgat
     2701 tctggaagtc tgccgaatgc tgacacgtgc actggacttg gaggaagtgc tgcccagagc
     2761 gcttcaagt
//
LOCUS       DAOQUC010000434         2763 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_43394_length_2763_cov_2.935007, whole genome shotgun sequence.
ACCESSION   DAOQUC010000434 DAOQUC010000000
VERSION     DAOQUC010000434.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2763
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43394_length_2763_cov_2.935007"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..815)
                     /locus_tag="PKH07_08395"
     CDS             complement(<1..815)
                     /locus_tag="PKH07_08395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HNT35002.1"
                     /translation="MATLATDIFLKGYQWPFDHDKIANAGSSLIDVLVQNEMINRGRR
                     VFMDFTSNPVATQGLDDFRLESLRPEALEYLQKSGALQNTPIQRLAHMNQPSIDLYKQ
                     MGVDLWKEPLEIGVCSQHCNGGFLVDAWWESNVKNLFVIGELAGTHGVKRPGGSALNS
                     GQVGGLRAALRIAHVYFDRADSMDDFADRASGVVKRRIAEVAQVRQCSSAARDCSQIR
                     QAIQRRMSEHAGMVRRREGVREALECAWREYKDIQKNGMKQSTPGYLDAVIVR"
     gene            complement(956..1720)
                     /locus_tag="PKH07_08400"
     CDS             complement(956..1720)
                     /locus_tag="PKH07_08400"
                     /inference="COORDINATES: protein motif:HMM:NF013086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HNT35003.1"
                     /translation="MIHCSEIDIEGIRVPVYSLNTVIVGSGAAGLNCAARLFREMEEL
                     GFDTPENEIAVVTEGIGHGTSNNSGSDKQTYYKLGTHGVEPDSPIDFAKTLTAAGSAH
                     GDLALMEGENSLRCFHHLVDIGVPFPHTASGSFVGYKTDHDPRQRATSAGPWTSRYMV
                     QKLIAELRRYGVQFFSHHHVLSVITEGGSERACGLLCADSRLETSDNHGFVLFNCRNV
                     VMAGGGPGDLYEISVYPKKQMGPFAALFELVQLQEI"
     gene            complement(1746..2549)
                     /locus_tag="PKH07_08405"
     CDS             complement(1746..2549)
                     /locus_tag="PKH07_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020596550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HNT35004.1"
                     /translation="MKVSLEGKRVALVTGAGQGIGRGIAVRLAQEGYSLAVNDIIADA
                     GNVQSGAYEVKATIERGGSVAEVFCADIASAKARETLVDEVFSRFGKIDLLVNNAGVA
                     PKERRDILNASEESFERLISINLQGPYFLTQLVANRMIQWKDEGKIETARIVFITSIS
                     AYTSSTARGEYCISKAALSMAAKLYADRLAEYGIPVIEIRPGIIETPMTSVVKDKYDK
                     MISDGLLPIKRWGKPDDIARVVSAFARGDLDYSTGECIEVGGGFGIRRL"
     gene            complement(2551..>2763)
                     /locus_tag="PKH07_08410"
     CDS             complement(2551..>2763)
                     /locus_tag="PKH07_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889061.1"
                     /note="NADH-dependent; catalyzes the conversion of
                     pyrimidines to 5,6-dihydro compounds in pyrimidine
                     degradation; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidine dehydrogenase"
                     /protein_id="HNT35005.1"
                     /translation="QDDLQSVLTNVSLTRSDLIATDPETMETSLEGVYAGGDIVNGGA
                     TIVQAIAEGMRAATAIGQWLMSNKEI"
BASE COUNT          680 a          831 c          620 g          632 t
ORIGIN      
        1 tcacgaacaa tgaccgcatc cagatatcca ggcgtgctct gtttcatgcc attcttctga
       61 atgtctttgt actcccgcca ggcacattcc agagcctctc gaacgccttc tctgcgacga
      121 accatgccag catgttcact catccgtcgc tggatagcct gccgaatctg agagcaatcg
      181 cgagccgccg aggagcactg gcgaacctgc gccacctctg cgatacgcct tttgactact
      241 ccggaagccc gatcggcaaa atcgtccatc gaatctgcac ggtcgaagta aacatgcgcg
      301 atcctcagtg cggcgcgaag ccctccaacc tggcctgagt tcagagccga cccgcccggc
      361 cttttcacgc catgagttcc agcaagctcc cctatgacaa agagattctt tacgtttgac
      421 tcccaccagg catccaccag aaagcctcca ttgcagtgct gcgaacacac acctatctca
      481 agaggctcct tccaaaggtc aacacccatc tgtttgtaca gatcaatact cggctgattc
      541 atgtgagcca gtcgctggat cggagtattc tggagcgctc ctgacttctg aagatactcg
      601 agagcctctg gtcgaagtga ctccaaacga aagtcatcca gtccttgggt ggcaacggga
      661 ttgctcgtga aatccatgaa tacacgacgg ccccgattga tcatctcgtt ctgcacaagc
      721 acatcaatga gcgatgaacc ggcgttcgca atcttgtcat gatcaaacgg ccattggtag
      781 cccttcagga agatatccgt agcgagcgtc gccatgcagt cgaaccatgg gttcagaaac
      841 tcctgaacat cccccccgtc agagtcggtg ctaaaatacc tcggaacaac ctgttgatag
      901 gttcccgaaa ggttccatcg cgggttcaga gaagccagtc caaactgtga ttccgtcaga
      961 tttcctgcag ttgcaccagc tcaaacaacg ctgcaaacgg acccatctgc ttcttgggat
     1021 agaccgatat ctcgtacaag tcccccggtc caccacccgc cataacgaca ttgcgacagt
     1081 tgaacagcac gaaaccgtga ttgtcacttg tctccagacg tgaatctgca cagagcagtc
     1141 cacatgccct ctcactgccg ccttccgtga taaccgagag aacatgatga tgactgaaga
     1201 actgaactcc gtaacgtctc agttcggcaa tcagtttctg aaccatataa cgggatgtcc
     1261 agggaccagc agaggttgcc cgttgtctcg gatcgtgatc tgtcttgtac cccacaaaac
     1321 tcccagaggc agtgtgcgga aaaggaaccc caatatccac cagatgatga aaacaccgca
     1381 gcgagttctc accttccatc aaagccagat caccatgggc agagcccgca gcggtcaagg
     1441 tcttcgcaaa gtctattggg ctgtctggtt caacgccatg ggtgcccaat ttgtagtaag
     1501 tctgcttgtc cgaaccggag ttgttggatg tgccatgccc aatcccttcc gtcacaacgg
     1561 cgatttcatt ctctggagtg tcaaacccga gttcctccat ctcgcgaaag agtcgagcag
     1621 cacaattcaa gcctgctgct ccactgccaa caatgaccgt attcagtgag taaacgggaa
     1681 cacgaattcc ctcaatgtct atttcgctac aatgaatcat cagcctggag acaccccttc
     1741 cctcttcaga gacggcgaat tccgaatcct ccaccaacct cgatacactc acccgttgaa
     1801 taatcaagat caccccgagc aaaagccgaa acaacccgtg caatgtcatc aggctttccc
     1861 caacgtttta tcggaagcag tccgtctgat atcatcttgt catatttgtc cttgacgaca
     1921 ctggtcattg gagtctcgat aatcccaggg cggatttcaa tgactggaat gccgtattcc
     1981 gcaagacgat cggcgtaaag tttcgctgcc atgctcagag cggctttcga gatgcagtac
     2041 tctccccgag ctgttgatga ggtataggcc gaaatggaag tgatgaagac gatacgcgcg
     2101 gtctcaatct ttccctcatc cttccattga atcatccgat tggccaccaa ctgggtaaga
     2161 aaatacggtc cctgcaggtt gattgagatg agcctctcaa aactctcctc tgatgcattg
     2221 aggatatccc gacgctcttt gggtgcgaca cctgcattgt tcacaagaag atcaatcttg
     2281 ccaaaacgcg aaaacacttc atcaaccaga gtttcccttg ccttcgcaga ggcaatgtca
     2341 gcgcaaaaga cctcagcgac gctgccacca cgttcaatgg tcgccttcac ctcgtatgct
     2401 ccagactgaa cattaccggc atcggcaatg atgtcgttga ctgcaagaga atagccttct
     2461 tgtgcaagtc ggacagcaat ccctcttccg atgccctgtc ctgctccagt aacgagcgcc
     2521 accctctttc cctcaagcga aaccttcact tcagatctcc ttattactca tgagccattg
     2581 accaatggcc gtggccgccc tcattccttc tgcaatggct tggacgatcg tggccccgcc
     2641 gttgacgatg tcgccaccag cgtaaacacc ttccagcgaa gtctccatcg tctcagggtc
     2701 agtggcaatc aaatcgcttc gagtgagact gacattggtc agcacgctct gcagatcgtc
     2761 ttg
//
LOCUS       DAOQUC010000435         2759 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_43494_length_2759_cov_2.389793, whole genome shotgun sequence.
ACCESSION   DAOQUC010000435 DAOQUC010000000
VERSION     DAOQUC010000435.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2759)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2759)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2759
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43494_length_2759_cov_2.389793"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..408
                     /locus_tag="PKH07_08415"
     CDS             <1..408
                     /locus_tag="PKH07_08415"
                     /inference="COORDINATES: protein motif:HMM:NF025839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4432 family protein"
                     /protein_id="HNT35006.1"
                     /translation="RDAEAEKGKEQFAQFDPPTPYYKEKVYYHDLVTEADGTVKTAIV
                     NENLEGGKFGVSVTYNQKELPFFTEWKMMGEATYVVGLEPANCRVEGRAKERERGTLI
                     ELEPGEIREYHVEIGVLEDAKAVDAFLSSMVKV"
     gene            412..1590
                     /locus_tag="PKH07_08420"
     CDS             412..1590
                     /locus_tag="PKH07_08420"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HNT35007.1"
                     /translation="MNDPRDISTGWEIPSEGYCDQPYVVKTSDGAWLCVMTTGRGVEG
                     ESGQHIVSIRSGDHGRTWSSLVDIEPADGPEASWAVPLIVPSGRIYVFYTYNAENLRE
                     VIADNEYARRRVDTLGEYAFRFSDDHGRTWSSERFFIPVREMEIDRRNPYGGKVRFFW
                     GVGKPIVHNGSVYFGFAKVGRFGDGFMAESEACFLCSDNILTESNPRKIRWETLPEGE
                     HGLRSPEGLIAEEPSVTSLSDGSLFCTYRTVASHPCQAYSRDGGRTWTPPEFMRYSPG
                     GSFVNHPRAANFVRRLDEGEWTGRFIYWFHNHNGRSYEGRNPVWLLGGVEKEGPEGKV
                     IHWGLPEVVLYAKDREIRISYPDFIEDAGRFFVTETQKTIARVHEVPRWLLSRLWEHC
                     "
     gene            1922..2587
                     /gene="rpsB"
                     /locus_tag="PKH07_08425"
                     /pseudo
     CDS             1922..2587
                     /gene="rpsB"
                     /locus_tag="PKH07_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008336545.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
BASE COUNT          634 a          673 c          819 g          633 t
ORIGIN      
        1 cgagatgccg aggcggagaa gggcaaggag cagttcgccc aattcgatcc accgaccccc
       61 tattacaagg aaaaggtcta ctatcacgat ctcgttaccg aagccgatgg aacggtcaag
      121 acggccatag tgaatgagaa ccttgagggt ggtaagtttg gcgtttcagt tacctacaac
      181 cagaaagaac tcccattttt taccgagtgg aagatgatgg gtgaggcaac ttacgttgtc
      241 gggctggagc cggcaaactg ccgcgttgaa ggcagggcaa aagaacggga acggggaacg
      301 ctgatcgaac tggagccggg agagattcgc gaataccacg ttgagatcgg cgttttggaa
      361 gacgccaagg cggtggatgc ttttctgtcc tccatggtga aggtctagtc gatgaacgat
      421 ccgagagata tctcgaccgg ttgggaaatc ccttccgagg gctactgtga tcagccgtat
      481 gtcgtcaaga catccgacgg cgcctggttg tgcgtcatga ctaccggtcg cggtgtcgag
      541 ggggaatcgg gtcagcacat cgtttccatc cgaagcggtg accatggtcg aacgtggtct
      601 tctctcgtcg atattgagcc tgctgatggt ccggaggcat cttgggccgt gcctctcatc
      661 gtgccttcag ggcgcatcta cgtcttctat acatacaacg ccgagaacct tcgggaagtg
      721 atcgcagaca acgaatatgc ccgacgccgt gtggacactc tcggagagta cgcctttcgt
      781 ttctcggatg accacggtcg gacatggtcc tcagagcgtt ttttcatccc agttcgggag
      841 atggagatcg atcgcaggaa tccttacggg ggtaaagtgc gctttttctg gggcgtaggc
      901 aagcccatcg tgcacaacgg atcggtgtat tttggttttg ccaaagtggg acgttttggt
      961 gatggcttca tggcggagtc cgaggcctgt tttctgtgca gtgacaacat actgactgaa
     1021 tccaacccca gaaagatccg ttgggagact cttccggaag gagagcatgg gcttcgttca
     1081 cccgagggtc tgatcgctga ggagccgagt gtgacctcgc tcagtgacgg ctctcttttt
     1141 tgcacatacc ggactgtggc aagtcatccg tgtcaggcct attcgcgtga tggcggccgc
     1201 acatggactc cacccgagtt catgcggtat tccccaggcg gttcctttgt gaaccatccg
     1261 cgggcggcca acttcgttcg aagactggat gaaggggaat ggacaggtcg ttttatttac
     1321 tggtttcaca atcacaatgg ccgaagctat gaaggccgga atccggtctg gctgctgggg
     1381 ggtgtcgaga aagagggtcc cgaaggcaag gtgatccatt gggggctgcc tgaggtcgtt
     1441 ctctatgcca aggatcgcga aatccggatc agctaccccg acttcatcga agacgccggg
     1501 cgcttttttg tgacagagac gcagaagacc attgcccgag ttcacgaagt gccacgatgg
     1561 ctgttgtctc ggctctggga gcattgctga accgacctat ctcccaattg cattggagcc
     1621 ttttcctgtc cttccatttc ctgcacgcaa ggaaagaaac ctctatcgca aaaagcgctc
     1681 aagctaaaga atttcttgca tttgcggttg aaatcatggt aagtatttct gttcgtcaac
     1741 caacccggta aggttggttg tgcgagcata ttgaccccgc acgttcggga acggttgata
     1801 agcgtcaccg tcggaacaac ggggccggtt cagtacccgc cttcagggat gtctatttgt
     1861 gtccctggtg cgtggtgtcg aatcgaaaaa tgaggttatt gaggaggatt ggacttcggt
     1921 gatggtaaca atgaagcagt tgttggagtc agggacccat tttgggcacc agactcggag
     1981 atggaacccc aagatgaaga agtacatctt tggggagaga aatgggatct acatcattga
     2041 cttgaagaag accgttcgtc ttctcaagga cgcctgccgt ttcgttcgcg acatggtggc
     2101 cgatggtggt gccgttcttt ttgttggaac caagaagcag gcgcaggata ccgtttttga
     2161 cgaggcgcgc cggtgcggtg cgtttttcgt caatcagaga tggctgggtg gtatgctgac
     2221 aaacttccag accattcaga agcgcatcaa gaggctgaag gaactggatc agatggctgg
     2281 cgatgggtca ctgagccatt acaccaagaa ggaagcctct atgatgatga aggagaggga
     2341 gaaacttcag aaatccctcg gtggcatcgc ggatatgaca aagcccccca gtgttctctt
     2401 tgtcacagac tcccggcagg agaggattgc cgtctccgaa gcccgtaagc tgggaattca
     2461 ggttgtagcc attgtggata caaacagcga tccggatgaa atagactatg tcattccagg
     2521 caacgatgat gccattcgag caattcgtct gctggcatcc aagatcgctg atgccgtcat
     2581 cgagggcaag gcaatccatc aggcacgcct tgagggaacg gaactcccac catcagatat
     2641 gcccgactcg gaaacaggtg aggaactgga agaagaggcg gtgccacagg cgccagtcgt
     2701 ttacaagcct gaaccgccag tagcccccgt tccggaaccg gaggttgttg tctcttctc
//
LOCUS       DAOQUC010000436         2757 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_43533_length_2757_cov_3.776462, whole genome shotgun sequence.
ACCESSION   DAOQUC010000436 DAOQUC010000000
VERSION     DAOQUC010000436.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2757
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43533_length_2757_cov_3.776462"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..808
                     /locus_tag="PKH07_08430"
     CDS             <1..808
                     /locus_tag="PKH07_08430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35008.1"
                     /translation="IYNKEISIDMAYQVFSWWQRACTDSVEIVPTLLLSMYDKNQTPV
                     FTEEELSNLCKFFRGKINPKHCAVYDVYPNRIQPGDLTVLTRYYPDGLIRVGLQPGEE
                     LTPPFKSAVEDTWSGFCHGIRNEQDWLQPGFGAESLRKWILERNNTTTPIAWDLIVVA
                     WDYSVTQRGEFPGYDDADKNMPLPAGRNLMAVKEILSLARPSSLAGFSSDLFILNENS
                     RSAQHDGGDGAFYRTLREGKQYNGYYAEAFREILAAYRHLAKGRIPEEGH"
     gene            complement(1118..2593)
                     /locus_tag="PKH07_08435"
     CDS             complement(1118..2593)
                     /locus_tag="PKH07_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNT35009.1"
                     /translation="MGIHRDQQSDKGCLGKDLMESDQLAPILLVDDDPQALGTGRSIL
                     FCGGFHSLICCKDAREVPLVLSQCPVRTVLLDLSMPYVSGQEILSLLSREYPDIPVII
                     VTAANDVSTAVECMRQGAFDYLVKPVEASRMIASVRRALELNELRRENEALRATLLTD
                     HLRNPEVFEEITTDHPTMRSIMQYAEAIAKTGKPVLITGETGVGKDLFAKAIHRASGC
                     SGEFVPVNIGGLDDFAFSDTLFGHLKGAYTGAETARRGLIEQASDGTLFLDEIGELSQ
                     ASQIKILRLLNQQEYRQLGSDVPKTSNARIIVATNQDLSRSLERETFRKDLYYRLQTH
                     HIHIPPLRERMSDLPLLLERFFSSATSELGKKIPPYPAELLAVLSAYSFPGNVRELES
                     MVFDCVAQHRTGRLSLKLFRDKTTHIPAVQGMGRARTITADSSFLSSLDKLPTLKEAA
                     ELLVEEALQRAKGNQTVASKILGITQSGLSKRLKRSHSLQD"
BASE COUNT          678 a          705 c          708 g          666 t
ORIGIN      
        1 gatctacaac aaggaaatct ctattgatat ggcgtaccag gtcttttcct ggtggcagag
       61 agcatgtacg gattctgtgg aaattgtgcc gactctgctt ctgagtatgt acgacaagaa
      121 tcagactcca gtcttcacag aagaggaact gagcaatttg tgcaaattct ttcgcggcaa
      181 gatcaacccg aagcactgtg ccgtctacga cgtttacccc aaccgcattc agccgggcga
      241 tctgactgtc ctgactcgtt actacccgga tggtctcatc cgagtcggtc ttcaaccggg
      301 cgaagaactt actccaccct tcaagagcgc cgtggaagac acatggagcg gattctgtca
      361 tggaatacgc aatgagcaag actggctcca gcctggattt ggcgccgagt cacttcgaaa
      421 gtggattctc gaacgcaaca acacgaccac accaattgcc tgggatttga tcgtggttgc
      481 ctgggactac agcgtcactc agaggggcga gtttcctggc tatgatgatg ctgacaagaa
      541 tatgccacta cccgctggtc ggaatctgat ggcagtcaag gagatactca gtcttgcaag
      601 gccttcctca cttgccggtt tcagttctga cctcttcatt ctgaatgaga attcccgcag
      661 tgcacaacac gacgggggtg atggggcgtt ctaccggacg cttcgcgaag ggaaacagta
      721 caacggttac tacgccgagg catttcgtga gatcctggcg gcttacagac atctggccaa
      781 gggaagaata cccgaagaag ggcactaagc aagaaacaga cactctgaga gatgcaacat
      841 ctctcagaat gcctgttctt ggattgcttt ttcccccaac ccggttgtcc cgtacctcgt
      901 ttctccaaat gcatcagcaa cttatgtgcc atcgggagac agttatccca atgcattgat
      961 aatagaaaca ttacctcggg gaacgcttac agaacaaatg atggcttact atgaattttg
     1021 gcatgcacgg atagttatag aagagataat tacttaaaat agaatagctt atatattcaa
     1081 tgacaagatc gagcgctatg ccatttgtca tggcattcta atcttggagc gaatgagagc
     1141 gcttgagacg cttgctcaag ccggactgag tgatcccaag tatcttcgag gccaccgtct
     1201 gatttccttt tgcccgctga agtgcttctt ctaccagcaa ctctgccgcc tctttcagtg
     1261 tgggcagttt gtcaagagat gaaagaaagg acgagtctgc ggtgatggtt cgagcacgtc
     1321 ccatgccttg tactgccgga atgtgcgtgg tcttgtccct gaagagtttg agtgaaagtc
     1381 ttcccgttcg gtgctgcgca acgcagtcga aaaccatgga ctccagttca cgaacgtttc
     1441 ctggaaatga gtatgctgaa agaacggcaa gcagttcggc aggatagggt ggtattttct
     1501 tccccaactc agaggtggct gaactgaaga aacgctccag aagcagtggc agatcgctca
     1561 tcctctcacg caaaggcgga atgtgaatgt ggtgcgtctg cagtcggtag tacaagtcct
     1621 ttcggaacgt ttctctctcc aatgaccgag acaaatcctg attggtggca acgatgatcc
     1681 gagcattgct ggtcttggga acatcggagc ccagctgacg atactcctgt tggttgagga
     1741 gcctgaggat tttgatctgc gatgcctggg agagttcacc gatttcatcg aggaagaggg
     1801 ttccgtcgga cgcctgctca atgagtcccc ttcgagcggt ttccgctcca gtgtaagccc
     1861 ctttgaggtg tccgaatagt gtgtccgaga aagcaaagtc atccagacca ccgatattaa
     1921 cgggaacaaa ctccccgcta catccactgg cacgatggat cgctttggca aagagatcct
     1981 ttccaacccc agtttctcca gtgattagaa ccggcttgcc agtcttcgcg atggcttcgg
     2041 cgtattgcat gatggagcgc atcgtcgggt gatcggttgt tatctcttca aagacttcgg
     2101 gattcctgag atgatcggtc agaagtgttg cacgaagagc ctcgttttcg cgtctcagct
     2161 cattcaactc caacgcgcgc ctgactgatg caatcatgcg gctggcttcc accggcttga
     2221 ccagataatc aaaagccccc tgtcgcatac attcaactgc cgtactcaca tcgttggcgg
     2281 cagtcacgat gatcacgggg atatcggggt actctctgga aagaagactc aggatttcct
     2341 gacccgatac gtagggcata ctgagatcca aaagtactgt tctgactggg cattggctca
     2401 gaacaagggg tacttcgcgg gcatccttgc aacagataag actgtgaaaa cccccacaga
     2461 agaggatgct tctgcctgtg ccgagcgcct gtggatcgtc gtccacaagc aaaatgggag
     2521 ccaactgatc tgattccatc aaatccttcc cgagacaccc cttgtcagat tgctgatctc
     2581 tatgaatccc catctctttc agcaactata tcaagagcat cggaggaagg caagaagcct
     2641 caggtctcat tggtttctgg tgaagagcca ggcttgagcg gaagtttcgg aacacggtat
     2701 ctgcaactga cggggcggtg attcacccat tcccaccctt catccgagcg tgccacg
//
LOCUS       DAOQUC010000437         2757 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_43535_length_2757_cov_3.456329, whole genome shotgun sequence.
ACCESSION   DAOQUC010000437 DAOQUC010000000
VERSION     DAOQUC010000437.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2757
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43535_length_2757_cov_3.456329"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="PKH07_08440"
     CDS             complement(<1..282)
                     /locus_tag="PKH07_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013706216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HNT35010.1"
                     /translation="MENSNADALSASQEDYLEAIFHIVRENQVARVRDISKRLNVKSS
                     SVTGALQSLSERDLINYAPYDLITMKPKGLSLAKNIVRRHNALRDFFIKV"
     gene            complement(403..1200)
                     /gene="budA"
                     /locus_tag="PKH07_08445"
     CDS             complement(403..1200)
                     /gene="budA"
                     /locus_tag="PKH07_08445"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HNT35011.1"
                     /translation="MFPRSKTRLLFFMVAILVCCPAFGLGRVERDILYQVSTLDALMA
                     GLYDGVQSIGELSRHGDFGLGTFDALDGEMVVLDGRFYQVRFDGTTTRVNKRFTTPFA
                     CVTFFDKDLTIPLTVPHDWGRIQGVLDGALSSRNAFHAIRIDGTFLFVKTRSVPRQEK
                     PYTRKLAEIAAEQSVFEMRNITGTIVGFFCPYYSKGANLPGFHLHFISQDRTQGGHVL
                     EASLLHGTLFVDETLGFQMSLPSTDSFRRLVIQEADKEAVSVVERGK"
     gene            complement(1236..2132)
                     /locus_tag="PKH07_08450"
     CDS             complement(1236..2132)
                     /locus_tag="PKH07_08450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35012.1"
                     /translation="MIRGKLFGLAVLPAFILVRYARRKIAWWYKNAVMRKKAESILSQ
                     LPFLQRRREIGSGNIGIVYDVMLNNGWRDEMQVLWQTLNPMPERVAVKIVPMESGSRQ
                     AETLCHLAPRIESATAASKPLALCPFLALGLLNHHQTREPFLVEIMPFLQAESLLKVL
                     RREKVQYREGIKELMRVFDTIFFLESQGCFERSLDNENVMVLPDGTWIRIDVDSAEMV
                     QAMPRYRMIRVARLTAEVLERLHGVPDRARLRSVLRRLQLTAKCPLKRYEGKPLPKKY
                     QGALFESPEEVRDALLSILEGA"
     gene            2397..>2757
                     /locus_tag="PKH07_08455"
     CDS             2397..>2757
                     /locus_tag="PKH07_08455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35013.1"
                     /translation="MKSLLTTLFITVAMAASAFGADLSTLEQAFEQAQTYVFGQSRQS
                     LATIEDWIRHNLDNEAERAEVAIRLAGLLGSETTLECKDFACRQLALIGTKDQIPALA
                     PLLTEPQTSDMARYALER"
BASE COUNT          693 a          774 c          668 g          622 t
ORIGIN      
        1 cactttgatg aagaaatcgc gaagcgcatt gtgacgacga acgatgttct tggccaggga
       61 aagccccttg ggcttcatgg tgatcaaatc gtagggagcg tagttgatta ggtcgcgttc
      121 ggacaggctc tgaagcgccc cggtcacaga ggaagacttg acattcaggc gcttggaaat
      181 gtctcgcaca cgagcgacct gattctctcg gacaatgtga aaaatcgcct ccagatagtc
      241 ctcttggctg gcactcaagg cgtcggcatt tgagttctcc attggcaccc tcttctcccg
      301 ctctgttacc tagagcgaca aatgttagct tggactaaaa atagccgtcg aaccgaacgg
      361 ttgtcaaggg aaatctgtgc gaggagaaac tgaacgaagc ggctacttcc cccgttcgac
      421 aacggagact gcctctttgt ccgcttcctg tatgacgaga cggcggaacg agtcggtgct
      481 cggaagactc atctgaaacc ccagcgtctc atcaacgaag agggttccgt gcaggaggga
      541 tgcttccaga acatgtcccc cctgagttct atcctggctg ataaagtgaa ggtgaaaacc
      601 cgggagattc gcccctttgc tgtaataggg gcagaagaaa ccaacgatcg tccccgtaat
      661 atttcgcatc tcaaaaacac tttgctctgc ggcaatctca gcaagtttcc gcgtatacgg
      721 tttttcctgg cggggaacgc ttcgcgtctt gacgaacagg aaagttccat caattcggat
      781 agcgtggaaa gcatttcgag agctcagggc tccgtccaga actccctgaa tgcgtcccca
      841 gtcgtggggt acggtaagag gtatcgtcaa gtccttgtca aaaaaggtta cacaggcaaa
      901 gggcgtcgtg aaccttttgt tgacccgcgt tgtcgtacca tcgaatcgaa cctggtaaaa
      961 acgtccatcc aagactacca tctcgccatc gagagcatca aaagttccca acccgaaatc
     1021 tccgtgcctg ctcagttcac cgatgctctg caccccatcg tagagacctg ccataagtgc
     1081 atccagggta gacacctgat aaagaatatc ccgttctacc ctgcccaggc cgaaagcggg
     1141 acaacagacg agaatcgcca ccataaagaa caacagccgt gtcttagatc ttggaaacat
     1201 gacattcctc ccgttgggaa agcgtctata gcctctcagg caccttcgag gattgagaga
     1261 agcgcgtccc gaacttcttc cggactctcg aacaaagccc cttggtactt cttcggcaaa
     1321 ggctttccct catatcgctt gaggggacac ttggcggtca gctggagccg cctcaaaaca
     1381 gaccgcagtc tggcgcggtc gggaacccca tgcaaacgtt ccagaacctc tgctgtcaaa
     1441 cgtgcaactc gtatcatcct gtagcgaggc atcgcctgaa ccatttcagc agaatcaacg
     1501 tcaattcgaa tccaggtacc gtcgggcagt accattacat tctcattgtc aagactgcgt
     1561 tcgaaacacc cctgcgactc gagaaagaaa atcgtgtcga aaacacgcat cagctctttg
     1621 atcccttccc gatactgtac tttttctctt ctcaggactt tcaacagcga ctcagcctgc
     1681 aggaaaggca ttatctccac aaggaacggc tcccgcgtct ggtggtgatt cagcaacccc
     1741 agcgcgagaa aaggacagag cgccaaaggt ttcgaagcag ctgtggcaga ttctatcctg
     1801 ggtgccagat gacacaatgt ttctgcctgt ctactcccag attccatcgg cacaatcttg
     1861 acggccacac gttcgggcat agggttcaaa gtctgccaga ggacttgcat ctcgtcgcgc
     1921 cagccattgt tgagcatcac atcgtagacg atgccgatat tcccagatcc tatttccctt
     1981 ctcctctgaa gaaaaggaag ttggctgaga atgctctccg ccttctttct catgacagcg
     2041 ttcttgtacc accaagcgat cttccggcgc gcgtaacgca ccaggataaa tgctggaagg
     2101 actgccaaac caaacagttt accgcgaatc atgacttgtt ccctttccga aagatagact
     2161 ctacgttcga tttgacagtg ctgtcaataa tctcatggta tgcttaccta ttgtaccaac
     2221 acaagacctc gatgcaaggg gacgaacccg aaagcacggg gtgttcgcaa agatcccacc
     2281 tctttgcagg tcttgacaaa gacttctctc tcacatagct tccaccaata catcatccaa
     2341 cggtgtgtct cttgagccat tcagctggcc aacgaagaac ccggacggag gggatcatga
     2401 aatcacttct cacaacgctc ttcatcactg tagcaatggc ggcgtccgca ttcggtgcgg
     2461 acctttcgac gttggaacag gcttttgaac aggcacaaac ttatgtgttc ggtcaaagcc
     2521 gccagtcact cgccaccatc gaggactgga tcaggcacaa ccttgacaac gaagcagaac
     2581 gtgccgaagt ggcgatccga ctggccggtc ttcttggttc tgaaacgact ctggaatgca
     2641 aggattttgc ctgccgtcaa ctcgcgttga tcggcaccaa ggatcagatt cccgcactgg
     2701 ctccattgct gaccgagcca cagacttcgg acatggctcg atatgccttg gaacgaa
//
LOCUS       DAOQUC010000438         2752 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_43654_length_2752_cov_3.347052, whole genome shotgun sequence.
ACCESSION   DAOQUC010000438 DAOQUC010000000
VERSION     DAOQUC010000438.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2752)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2752)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2752
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43654_length_2752_cov_3.347052"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..301)
                     /locus_tag="PKH07_08460"
     CDS             complement(<1..301)
                     /locus_tag="PKH07_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008619500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein"
                     /protein_id="HNT35014.1"
                     /translation="MQWFLARKCGIEGVGILKAKQTRSRLFLLAAVIWIAVVATSLIW
                     NWYESEHAVITAAISEARIAFEKDIVYRRWASSHGGVYVPPSETTPPNPYLAHIPD"
     gene            complement(737..2077)
                     /gene="gdhA"
                     /locus_tag="PKH07_08465"
     CDS             complement(737..2077)
                     /gene="gdhA"
                     /locus_tag="PKH07_08465"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013162370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HNT35015.1"
                     /translation="MAYVDDVMDLVVRRNPGELEFHQAVKEVLDSLRPVMERHKKYQK
                     NSILERFVEPERVLMFRVPWVDDKGNVQVNRGFRVQMSSALGPYKGGLRFHPSVYLGI
                     IKFLAFEQILKNSLTTLPMGAGKGGSDFDPKGKSDMEVMQFCQSFMTELCRHIGADVD
                     VPAGDIGVGGREVGYLFGQYKRIRNEFTGVLTGKGLGWGGSLIRPEATGYGAVYFAEQ
                     MLAMRGDEIKGKVCTVSGSGNVAQYTVEKLNQLGATAVSLSDSNGTIHDPDGINAEKL
                     AWVMDLKNVKRGRIKEYADKFKGSKYLEGVNPWGIKCDCAFPSATQNEISGEDAKTLV
                     KNGCCLVAEGANMPTDPDGVDVFLAKKVFYGPGKAANAGGVAVSGLEMSQNSMRLSWT
                     REEVDSRLKGIMTAIHTASYEASQEYGDPGNLVMGANIAGFVKVADAMLDHGVV"
     gene            2432..>2752
                     /locus_tag="PKH07_08470"
     CDS             2432..>2752
                     /locus_tag="PKH07_08470"
                     /inference="COORDINATES: protein motif:HMM:NF020050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged-helix domain-containing protein"
                     /protein_id="HNT35016.1"
                     /translation="MDINTRRKIVAILRELHDSERPLGSTRIGEALQAQGIDLSERTV
                     RHYLGLTDKAGLTANMGKRGRKLTPQGERELESALVVDKVGFVAARVDSLAYQMSFSL
                     SKRKG"
BASE COUNT          682 a          793 c          644 g          633 t
ORIGIN      
        1 gatccggaat atgcgccagg taaggattcg gcggtgtcgt ctctgaagga ggaacataca
       61 cccctccatg agaagacgcc caccttcgat agacgatgtc cttctcaaat gcaatccgtg
      121 cctctgagat tgccgctgtg atgactgcat gctctgactc ataccagttc cagatcagtg
      181 aagtcgccac tacggcgatc cagataaccg cggcaagaag aaaaagtctg cttcgcgtct
      241 gcttggcttt tagaataccc acaccctcaa ttccgcactt tctcgccaga aaccactgca
      301 cagaaaagtt ccgttatcct cttcataatg tactaccacc aaggaaacgt gtcaaaggaa
      361 agcgatactg aggagcaccg gctcagttac ggagggggtg gaggtatgct tcggtagtag
      421 aggctccgcg acactgccga agcgagaaca gcgagacagc gggcttcggt agcattgctc
      481 cgaagcctgc cctgagcgca gccgaaggga ctccgcgttg cttccgaagc ataccttgcc
      541 tgcatcacct gcataaccga gccagtacaa aaaaagcgac actgaggctc tgagtgctgt
      601 gggcttgctt tcgggaaacc ccgaccatag aaaacggtca ggggcacggt acaccgcacc
      661 cctgagtctt atctacgatc aacttgttca atctgtctga aactatcaat ggtcaatcgt
      721 ccattgacaa ttcactctag acaactccgt gatccagcat ggcatccgcc accttgacaa
      781 atccggcaat gttggcaccc atgaccagat tccctggatc gccgtattcc tgtgatgcct
      841 cgtaagaggc cgtgtgaatc gcggtcataa tccctttgag gcgactgtca acttcttctc
      901 tcgtccagga cagcctcatg gaattctgcg acatctccag accggaaacg gccacaccac
      961 cggcgttggc agccttgcct ggtccataga aaaccttctt ggccaggaac acatctacgc
     1021 catcggggtc agtcggcatg ttggcgcctt cagcaaccag gcaacaaccg tttttcacca
     1081 acgtcttggc atcctcacca gagatctcat tctgtgtcgc agaggggaat gcacagtcgc
     1141 acttgatgcc ccatggattg accccttcga ggtacttgga acctttgaac ttgtcagcat
     1201 actccttgat acgtccacgc ttgacgttct tcagatccat aacccaggcc agcttctcgg
     1261 cattgatgcc gtcgggatca tgaattgtcc cgttcgaatc cgaaaggcta actgccgtgg
     1321 ctcccaactg gttgagcttt tcgaccgtgt actgcgcaac gttacccgaa ccggagacgg
     1381 tgcaaacctt cccctttatc tcatcgccac gcatcgcaag catctgctcg gcaaagtaga
     1441 ccgcaccgta gccagttgcc tcaggtcgaa tcaacgaacc accccagccg aggcctttgc
     1501 ccgtcagaac accggtgaac tcattgcgaa ttcttttgta ctgcccaaac agatagccga
     1561 cttcacgacc accaacgccg atatctcctg cgggaacatc cacgtcagca ccaatatgac
     1621 gacatagttc cgtcatgaac gactgacaga actgcatcac ttccatgtcc gatttgccct
     1681 tgggatcgaa atccgagccg cctttgcctg ctcccatcgg cagcgtcgtc aaagagttct
     1741 tcagaatctg ctcgaaagcc agaaacttga tgattcccag atagacactc ggatgaaaac
     1801 gaaggccgcc cttatagggt cccaaggcgc tgctcatctg aacgcgaaaa ccacggttga
     1861 cctggacgtt gcccttgtcg tcaacccagg gaacgcggaa cataagcact cgctcgggct
     1921 ccacgaatcg ttcaagaatg ctgttcttct ggtatttctt gtgccgctcc atgactgggc
     1981 gaagtgaatc gagaacctct ttaaccgctt ggtgaaactc cagttcacca ggatttctcc
     2041 tgaccaccaa atccatcaca tcatcaacgt acgccatctc ttgctccttc ttctttccgc
     2101 cacttctgtg gctttgacac tccaagttcg gagtccagtt tctatcaaac gcatctacct
     2161 ctttatcggt aatgcattgc ctataattca tagagaaaag gacttgtcaa gaaaaatatt
     2221 atctttgata ttaagatatt gatttagaat gctttatacc gtcacattcc caggagatat
     2281 caaaatatca aaaaatgaca acttgtcggc aagaatgccg tctaaactgc ttgacaactg
     2341 gaagatcctt ggatatggtt gccgatgatg ttgccttcta aagtgtcttg gcaacctagt
     2401 ctatctcatg tgctgaggca ggcaggacgt tatggacatc aacaccagac gcaagatagt
     2461 cgccattctc cgcgaacttc acgattccga gcggcctctc ggcagtacgc gcatcggtga
     2521 agctctgcaa gcccagggga tcgatctgag cgaacgcact gttcggcact accttggcct
     2581 gaccgataaa gccggcctta cagcgaatat gggcaagaga ggccggaaac tgactcccca
     2641 aggcgagaga gagttggaaa gcgcgctggt agtggacaag gtcggctttg tggccgctcg
     2701 cgttgactct ctggcatatc agatgtcctt cagtctcagc aagcgcaaag gc
//
LOCUS       DAOQUC010000439         2744 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_43865_length_2744_cov_2.906657, whole genome shotgun sequence.
ACCESSION   DAOQUC010000439 DAOQUC010000000
VERSION     DAOQUC010000439.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2744)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2744)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2744
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43865_length_2744_cov_2.906657"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..310)
                     /locus_tag="PKH07_08475"
     CDS             complement(<1..310)
                     /locus_tag="PKH07_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014377417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35017.1"
                     /translation="MAKSIGSGATEGRDKAVDLAVSQIEKLYGKGAIMRLSEKPQFEV
                     SVIPTGSLALDAALGIGGLPRGRVVEIFGPESSGKTTLALHAVAEAQKANGVAAFVDA
                     E"
     gene            complement(327..659)
                     /gene="yajC"
                     /locus_tag="PKH07_08480"
     CDS             complement(327..659)
                     /gene="yajC"
                     /locus_tag="PKH07_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013164116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HNT35018.1"
                     /translation="MLFLFTALAHAQQVAPPAPGGQFSPSNLILLYAIIVGGFVFLII
                     LPQRRKQKDHDRKLEALKQNDRVITNGGIFGTISRIKGNVVELKIADNVKVDILKSCI
                     SSTVEEEK"
     gene            complement(662..1447)
                     /locus_tag="PKH07_08485"
     CDS             complement(662..1447)
                     /locus_tag="PKH07_08485"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNT35019.1"
                     /translation="MQKTPPEMYRKFAEIYDVVMRQIDYITWESHVTSLAKRWCPNMR
                     DILSVACGTGSLEVLLAQRDYNVFCLDLSEDMLRVAERKFEVLPEKPPTKCACMTNFD
                     LAKQFDVVLCLYDSLNYLTRPEQVQGAFERAYVHLRPGGIYIFDVTTEYNILQHFAEY
                     TFAENLDGYSYIWDNAYNIGTKLCTSEVTIFEEVEPGLYRRFNERHDQRMYANKDISR
                     WLNQAGFKLQGIYDGFSIAPPKDHSERIHFVAQRPENDNEEGV"
     gene            complement(1454..2569)
                     /locus_tag="PKH07_08490"
     CDS             complement(1454..2569)
                     /locus_tag="PKH07_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012003311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HNT35020.1"
                     /translation="MNEQDSQIPAQPLRSTVAVLKTEPETVLDDYTQLMRLAEFEKHL
                     DKNAPVILKDNISWHLMFPGANTTPWQLEAVIRTLLDAGYKELICVQNQTVVTNAFKG
                     EALNKYLGVLEKYNIPVLYNFRPQDMKWIRYQPKAKMLVLDKIFPEGILIPDFFPGKN
                     VLHLPTVKCHIYTTMTGAMKNAFGGLLNTRRHYTHSVIHETLVDLLNIQKEIHAGIFT
                     VMDGTTCGNGPGPRTMIPVIKDYLLASGDSVAIDAISAKLMGFDPMSLKKIRLATEAG
                     LGTGILENINVVGADISQENFGFTVGDNAASTVGDMLWFGPLKWLQHLMFHTPLVYFF
                     IWGSFVYHDFIWYPNVGKPIVQDWMKTKWGQLFQKYE"
BASE COUNT          626 a          737 c          693 g          688 t
ORIGIN      
        1 gctcggcgtc tacaaaggca gcaaccccat tggctttctg tgcctcagca accgcatgaa
       61 gtgccagtgt tgtcttgccc gaggattccg gtccgaaaat ctccacaacg cgaccccggg
      121 gcagcccacc aatacccaaa gcggcatcga gcgccagaga accggttgga atcacggaca
      181 cctcgaactg cggcttttcc gacagccgca taatcgcgcc tttgccatag agtttctcga
      241 tctggcttac cgccaaatcc actgccttgt cacgaccttc tgttgcacca ctgccgatac
      301 tctttgccat tgtctgaaac ctccccctat ttctcttcct cgactgttga actgatacac
      361 gacttcagga tatccacctt gacgttgtcg gcgatcttca gctcaaccac attacctttg
      421 atccgagaga tcgttccgaa gattccgcca ttggtgatga ccctgtcgtt ttgtttgagc
      481 gcttccagct ttctgtcatg atccttttgc ttccggcgtt gaggcaggat gattagaaac
      541 acaaatcctc cgacaatgat cgcgtacaaa aggatcaaat tcgagggtga gaactgtccc
      601 ccgggcgctg gtggcgcgac ctgctgggca tgggcgagag cggtgaatag gaacaacaca
      661 atcaaactcc ttcttcgttg tcgttttcgg gacgttgggc gacgaagtga atgcgttccg
      721 aatgatcctt tggaggagca attgagaatc cgtcgtagat tccctgcaat ttgaatccgg
      781 cctggttcag ccagcgactg atgtccttgt tggcgtacat ccgctggtca tgccgctcgt
      841 taaagcggcg gtacaagccc ggctccactt cctcaaagat ggtgacttcg gaagtgcaca
      901 gtttcgtccc aatgttgtag gcattatccc agatatagga ataaccgtcc agattttccg
      961 caaaagtgta ttccgcaaaa tgctgcagaa tgttgtactc ggtggtcaca tcaaaaatgt
     1021 aaatcccgcc cgggcgcaga tgaacataag cccgctcaaa ggcgccctgc acttgttccg
     1081 gtcgggtcag atagttcagg ctgtcataga gacacaagac gacatcgaac tgtttcgcca
     1141 gatcgaagtt ggtcatgcag gcgcatttgg tcggtggttt ttcgggaagc acttcgaatt
     1201 tccgctccgc gactcgaagc atgtcttcgg acagatccag acagaagaca ttgtagtcac
     1261 gttgcgccaa cagcacttcg aggcttccgg tgccacaggc aacactcaga atgtcgcgca
     1321 tatttggaca ccaacgtttg gcgagactcg tgacgtgtga ctcccacgtg atgtagtcaa
     1381 tctgacgcat gaccacgtcg tagatctcag cgaatttccg atacatttca ggtggagtct
     1441 tctgcacaga acctcactcg tatttctgga acaattgtcc ccacttggtt ttcatccaat
     1501 cttgaacgat gggctttcca acgttggggt accagatgaa atcgtgatac acaaatgagc
     1561 cccagatgaa gaaatagacc aagggcgtgt gaaacatgag atgctgaagc cacttcaggg
     1621 gtccaaacca cagcatgtcc ccgaccgtgc ttgccgcgtt gtctccaacg gtaaatccga
     1681 agttctcctg cgaaatgtcg gctccgacaa cattgatgtt ctcaagaata ccggtaccga
     1741 gccccgcctc cgtggccaaa cgaatcttct tgagactcat cgggtcaaag cccataagtt
     1801 tcgcggagat tgcgtcaatc gctacactat ctccactggc cagcaagtaa tctttgatga
     1861 ccggtatcat ggttcggggt ccgggaccat tgccgcaggt ggtgccgtcc atcaccgtga
     1921 agattccggc gtgaatctcc ttctgaatgt tcagcaaatc aacaagggtc tcatggatga
     1981 cgctgtgggt gtagtgtcgt cgggtgttca gtagtccacc gaaagcattc ttcatggctc
     2041 ccgtcatcgt ggtgtagata tgacacttca ctgtgggaag atgcaggacg ttctttccgg
     2101 ggaagaagtc cgggatcagg atgccttccg gaaagatctt gtcaagtacc agcatcttgg
     2161 ctttcggctg gtaccgaatc catttcatgt cctgcgggcg gaaattgtag agcaccggga
     2221 tattgtactt ttccagaacg ccaagatact tgttgagcgc ttcgcctttg aatgcgttgg
     2281 tgactacggt ctgattctgg acacagatca gctctttgta acctgcatcc agaagcgttc
     2341 ggatgaccgc ttccaactgc cagggcgttg tgttggctcc tggaaacatc agatgccagg
     2401 agatgttgtc tttcagaatg accggagcgt tcttgtcgag atgcttttca aactccgcca
     2461 gtctcatcag ctgagtgtag tcgtccagta ctgtttccgg ttcggtcttc agaacggcga
     2521 ccgttgaacg caacggctgc gccgggattt gactgtcttg ctcgttcacg gtttcttctc
     2581 ctttcacaaa ttacaattca aagattccag aaaaacccaa gaaggcatcc ttggtgtcct
     2641 atggagtccc ggcttggccg ctcaggccac tggctgtctt ggtagcgcca tacttgtttg
     2701 tcagagcgcc tcgacgatca gagaataaag accgcagcgg cgat
//
LOCUS       DAOQUC010000440         2731 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_44190_length_2731_cov_3.166667, whole genome shotgun sequence.
ACCESSION   DAOQUC010000440 DAOQUC010000000
VERSION     DAOQUC010000440.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2731
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44190_length_2731_cov_3.166667"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..705
                     /locus_tag="PKH07_08495"
     CDS             <1..705
                     /locus_tag="PKH07_08495"
                     /inference="COORDINATES: protein motif:HMM:NF013641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HNT35021.1"
                     /translation="GAGETGQQAARNMQARGVKKVIVTNRTLDRAAPLAQCFKCEILP
                     FQHLDEGLQAADIVISCTSSPEPILTRQTVTSAMAKRAQSDPLMLIDLAVPRDIEESV
                     AGIAGVALFNIDDLQSVVEANAEKRMAEAEKAHILVNKAAEKFADWQRTLIAVPTIRE
                     LQTLFSRIQKEELAKYSEQVTPEELQRIEKVSGAITRRIAQLAISRMKEASGRPNAQA
                     FFESVQRLFDLEDTKQ"
     gene            702..1616
                     /gene="hemC"
                     /locus_tag="PKH07_08500"
     CDS             702..1616
                     /gene="hemC"
                     /locus_tag="PKH07_08500"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR00212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HNT35022.1"
                     /translation="MKDILRIGARASQLSLRQAEWVSTQLTVHSPQLHIEITTITTAG
                     DRDQDRKFADMGVTGVFTREIDRALLEGKIDVAVHSLKDLPTIPTQGLTLSAVPCRED
                     PRDAFISLDGRTLVEMDPDAKIGTSSVRREAQLRVLRSDLQIVALRGNVDTRIRKLRE
                     SKQYDGIIIAYAGLKRLDRADEATEILETERWLPAPGQGALALVTRESDETIRALVQP
                     LDDPDSHLGIVAERTLLESLGAGCHVPVGAYARIHSTGVTLDGFISEVDGSSFVRDRI
                     DGRREKAAMLGRTLAQTLREQFDRTRKS"
     gene            1613..>2731
                     /gene="cobA"
                     /locus_tag="PKH07_08505"
     CDS             1613..>2731
                     /gene="cobA"
                     /locus_tag="PKH07_08505"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF004790.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HNT35023.1"
                     /translation="MTETAHGIGVVWLVGTGPGDPDLITLKGLRCIAEADVIVHDRLI
                     PHVLLNHAKEKCVLIDVGKRPDHHTLQQEQINELLADQAAQGKRVCRLKGGDPLVFGR
                     GSEEALFLAERGIGFEIVPAVTAGVGALAYAGIPLTHRQIASCAAFITGHEDPTKPES
                     AIAWEALASFPGTLVFYMGVRHLQQLTANLIQYGLSPSTPAAVIENGTTPRQKVLVAD
                     LASLARRAEQQQFRPPSLIVVGEVVALRREMSWFEKRPLFGKTILVTRSREQASELVA
                     RLAELGAEVFQAPAIGIESLAQTTPMREAAASLEDFDWIVFTSPNAVDAFWGALRLER
                     RDARAFAAVKVASIGPSTAERLLQHGIVSDLLPEHFVSE"
BASE COUNT          650 a          715 c          796 g          570 t
ORIGIN      
        1 ggtgcagggg agaccgggca gcaggccgcg cgcaatatgc aggcacgcgg cgtgaagaag
       61 gtcatcgtca ccaaccgaac cttggatcgg gcggctcccc tggcacagtg tttcaaatgt
      121 gagattcttc cttttcaaca cttggatgag ggattgcagg cggctgatat cgtcatcagt
      181 tgcacgagtt cacctgaacc cattctgacg cgacagaccg taacatctgc tatggcaaag
      241 cgcgctcaat ccgatccctt aatgctcatc gatcttgctg tgcccagaga catcgaggaa
      301 agtgtggcgg gtattgccgg tgtggcgctc ttcaacattg acgatctgca gtccgttgtt
      361 gaggcaaatg cggagaagcg tatggcggaa gccgaaaagg cgcatattct ggtgaacaaa
      421 gctgccgaga aatttgccga ttggcagaga accctcatcg ctgttccaac gatccgcgag
      481 ttgcaaactc tctttagccg cattcagaaa gaggaacttg ccaagtattc cgaacaggtc
      541 acaccagaag agctgcagcg aattgaaaag gtctcagggg cgatcacccg acgcattgcc
      601 caactggcta tctcccgaat gaaggaagcg tcaggcagac ccaatgcgca ggcgttcttt
      661 gagagtgttc agcggctctt cgatctggaa gacaccaagc aatgaaagac atcctacgca
      721 tcggcgcacg tgcaagccaa ctcagcctca ggcaggcaga atgggtctcc acacagctga
      781 ctgtgcactc tccccaattg cacatcgaga taacaaccat cacgacagcg ggcgacagag
      841 atcaggatcg aaagttcgct gacatggggg tcacgggtgt tttcactcgc gaaatcgatc
      901 gcgctttgct tgaaggcaag atagatgttg ccgttcacag cctgaaggat ttgccaacaa
      961 tcccaacgca gggattgact ctcagtgccg tcccgtgtcg ggaagaccca cgggacgctt
     1021 tcatcagcct ggacggaaga acgcttgtcg agatggatcc agacgcgaag ataggaacca
     1081 gcagcgttcg cagagaagcg cagttgcggg ttcttcgaag cgatcttcag attgtcgctc
     1141 ttcggggcaa cgtggatacg agaatccgga aactccgcga gagtaagcag tatgacggga
     1201 tcatcatcgc gtacgcaggc ctcaagcgtc tcgacagagc ggacgaggcg acagagatct
     1261 tggaaacgga gcgttggctc cctgcgcctg gacaaggcgc gctggcatta gtcacaagag
     1321 agtccgacga gacgatcagg gcactcgttc aaccgctgga tgatccggat tctcaccttg
     1381 gtattgttgc cgagagaacc cttctggagt ccttgggcgc cggatgtcat gttccagtcg
     1441 gagcgtacgc caggattcac tccaccggag tgactctgga cggcttcatc tcggaagttg
     1501 acggaagttc ctttgtccgc gacagaattg acgggagaag agaaaaggcg gcgatgcttg
     1561 gaaggacact tgcgcagacg ctgcgcgagc agttcgatag gacaaggaaa tcatgactga
     1621 aaccgcacat ggaatcggcg tcgtctggct cgtgggaacc ggtccgggag atccggactt
     1681 gatcactctg aagggcttga ggtgcatcgc tgaggcagat gtgatcgtgc atgaccgtct
     1741 cattccgcat gtgcttctca accatgcgaa agagaaatgt gttctgatag atgtgggcaa
     1801 acggcctgac caccatactc tgcaacagga gcagatcaac gaacttcttg ccgatcaggc
     1861 agcgcaggga aagagagttt gtcgtctcaa aggtggcgat cctctggttt tcggcagagg
     1921 tagcgaagaa gcgctctttc ttgcagagcg gggcattggt ttcgagattg ttccagcggt
     1981 gacggcgggt gtgggtgctc tcgcttacgc tggcattccg ctgacgcata gacagatcgc
     2041 ttcatgcgcc gcattcatca cgggtcatga ggatcctacc aagccagaaa gcgcaatcgc
     2101 atgggaggct ctggcatcct ttcctggaac acttgttttc tacatgggag tcagacatct
     2161 tcagcagctg actgcgaatc tgatccaata cgggctctct cccagtacac ccgctgcagt
     2221 tatcgagaac ggaacaacgc cccgccagaa ggtcttggtg gcagatttgg caagtcttgc
     2281 acgacgtgca gaacaacagc aattcaggcc tccttcattg atcgtggtgg gggaggtcgt
     2341 cgcacttcga agggagatga gttggtttga aaaacgacca ctcttcggga agacaattct
     2401 tgtcacgcga tcccgagaac aggcatccga gcttgtggca agacttgcgg aacttggtgc
     2461 tgaagtgttt caagcccctg cgattggcat cgagagtctc gcacagacga cacccatgag
     2521 agaggcggca gcatcattgg aggacttcga ctggatcgtc ttcacgagtc ccaacgcagt
     2581 tgacgccttc tggggagcac tccggctgga acgccgagat gcccgcgctt ttgcggcagt
     2641 caaagttgca tccataggcc cttcgaccgc ggagagactc ttacagcacg gtatcgtctc
     2701 agatcttctg cccgagcatt ttgtctcgga g
//
LOCUS       DAOQUC010000441         2724 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_44378_length_2724_cov_2.418509, whole genome shotgun sequence.
ACCESSION   DAOQUC010000441 DAOQUC010000000
VERSION     DAOQUC010000441.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2724)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2724)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2724
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44378_length_2724_cov_2.418509"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="PKH07_08510"
     CDS             <1..224
                     /locus_tag="PKH07_08510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35024.1"
                     /translation="PPPRRFVKNYFEFFGGLQMTRNLSGRLPDPHPGLLREGEGVAEQ
                     TPCDPRAIRQCSRPTGGFLNGVFSFEPFD"
     gene            274..1605
                     /locus_tag="PKH07_08515"
     CDS             274..1605
                     /locus_tag="PKH07_08515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35025.1"
                     /translation="MKQFGHFLLLISTIVSFSSCSAFQKGGESMREIGNFFEHVDTGV
                     SLPEDAVYPKGRIFPYMGYSGIHVEDATNGFTVAGHHYGSLTLQKEKLHLAKVAGLSY
                     IFGVGLDGTFVKEPPLTYTPQLLRDTVRQQVLEVVNDPFVLWWYVRPEELRMWRPEED
                     EYLRIATETIRQNDPHDRPIWMYDPNHRNAESLMKTGRYLDIIGKGCYANAAGHQDSR
                     IWIRWSIDQETTACRELEKLDGRPRTPLVMPQMSKDPDDPALDGMIPTWARHDVYLGL
                     MAGAKGVCIFSLFPRAAITRTHKIHYEAFSRIGKELTGELNLGKVFLFGEERSDLVIQ
                     QTEGPATVELYTGPKNKLEENTTSQEERQRHLTTYPALMSKEISYAGSRYLFSCNSTQ
                     EQIVCEVSGIPQEGVIIEKVFDGKRVDAPDGSLTLTFDPWQARCIRISRAE"
     gene            1651..2640
                     /locus_tag="PKH07_08520"
     CDS             1651..2640
                     /locus_tag="PKH07_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017302454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HNT35026.1"
                     /translation="MQMDAPRNRTLECSAEELSVLSSALLQLSGPTDLQEIADRVIHQ
                     DFREACPLLPRSFADLLILDPPYNLTKNFNGRVFRAQEAEEYTSWFESMLATVLPSLR
                     PNASVYVCSDWRTSTLVFPVLDRHLHVRNRITWEREKGRGAKANWKNNTEDIWFCTLS
                     DSYTFNVDAVKLKRRVLAPYRHEDGRPKDWDESANGDYRLTHPSNLWSDITIPFWSMP
                     ENTDHPTQKPEKLIAKLILASSNPDDFVFDPFLGSGTSAVVARKLQRRYCGIELDWEY
                     CCWALKRLEAARTDADIQGYSDGVFWERNGISTFKSVVPIRIPMMMKTVKVKP"
BASE COUNT          672 a          723 c          743 g          586 t
ORIGIN      
        1 accccccccc ccgccgcttt gtcaagaact attttgagtt ttttggcggg ctccagatga
       61 cgcgcaatct cagcgggaga ttgccagacc ctcatcccgg ccttctccga gagggagaag
      121 gagtcgccga acagactccc tgcgatccca gggcgatacg ccagtgctcc cgaccaacgg
      181 gcggttttct gaacggcgtc ttttcatttg aaccctttga ctgaccggtg ctattgttcc
      241 tcgatggagc gagcgaggca cgggaggctt gagatgaaac agtttggaca tttcctcctg
      301 ctcatcagca caatcgtgtc attctcaagt tgctcggcgt ttcagaaagg tggggaatcc
      361 atgcgcgaga tcgggaactt ctttgagcat gtggacacgg gagtatcgct tcctgaggat
      421 gctgtttacc ccaagggacg gatatttccg tacatgggtt attccggaat tcacgtcgag
      481 gacgccacaa acggcttcac tgtggccgga catcactacg gttccctgac gctgcagaag
      541 gagaagttgc atctggcaaa agttgcggga ctttcctaca tcttcggcgt tggtttggac
      601 gggacattcg tcaaggaacc tccgctcacg tatacaccgc aacttctgag agacactgtc
      661 cgacaacagg ttcttgaggt ggtgaacgat ccttttgtgc tctggtggta cgtccgtccc
      721 gaagaactcc gaatgtggcg accggaagaa gacgagtatc tccgaatcgc cacggaaacc
      781 attcgccaaa acgatcctca cgacagaccg atttggatgt acgatccgaa tcatcgcaat
      841 gcggaatccc ttatgaaaac gggacgttat ctggacatta tcgggaaagg ctgctacgcc
      901 aacgcggcgg gacatcagga cagccggatt tggattcgct ggtcaatcga tcaggaaacg
      961 actgcttgca gggaactgga aaaacttgat ggcagaccca gaacaccgct cgtcatgccg
     1021 cagatgtcca aagatccaga tgatccggcg ctcgacggca tgatccccac ctgggcgcgt
     1081 catgatgtgt atctcggctt gatggctggc gcgaaaggcg tctgcatctt ctcactgttc
     1141 ccacgcgctg caattacgcg aacgcacaag attcactatg aagcgttcag ccgcatcggc
     1201 aaggaactga cgggcgagtt gaacttggga aaggtcttcc ttttcggaga ggaacgcagc
     1261 gatcttgtga tccaacagac tgaaggacct gcgaccgtcg aactctacac cggccccaag
     1321 aacaaactcg aggaaaacac aacgagtcaa gaggaacgac aacgccacct gacaacctac
     1381 cctgcactga tgagtaagga gatctcttac gcgggaagcc gctatctgtt ctcgtgtaat
     1441 tccactcagg aacagatcgt ctgcgaagtg tcaggtatcc cacaggaagg cgtcatcatt
     1501 gagaaagtct tcgacggcaa acgcgttgac gcacccgatg gaagcctcac actgactttc
     1561 gatccctggc aggcaaggtg tatcaggata tcaagggcag agtagaatgc tcctggcgag
     1621 atctcaacgg ctgggatggc tctggagaaa atgcagatgg atgcaccaag aaatcgaaca
     1681 ctggaatgtt ccgccgagga gttatctgtt ttgtcctcag cgttgctcca actgtcgggt
     1741 cctacagatc tgcaagagat agcggatcgg gtcatccacc aggattttcg agaagcgtgc
     1801 ccactccttc cgcgctcctt tgcagacctt cttatccttg acccgcccta caatctcaca
     1861 aagaacttca acgggcgcgt tttccgcgcc caggaagccg aggagtacac ctcatggttc
     1921 gagagcatgt tggcaactgt cttgccctcg ttacgcccga atgcttcagt ctacgtatgc
     1981 agcgactggc ggacgtccac cctggtcttc ccagttctgg ataggcatct ccatgtccgc
     2041 aacagaatca catgggaacg cgagaaagga cgcggtgcaa aggcaaattg gaagaacaac
     2101 acagaggaca tctggttctg cacactgagc gattcttaca ccttcaatgt tgacgccgtg
     2161 aagctgaagc ggagagtcct cgcgccatat cggcacgaag atggcagacc aaaggattgg
     2221 gacgagtccg ccaacggcga ttataggttg acccatccgt cgaatctgtg gtctgacata
     2281 acgattccct tctggtctat gccagagaac actgaccatc ccacgcagaa gccggaaaaa
     2341 ctcatagcca agctgatttt ggcaagttcg aatccggatg acttcgtttt cgatccattt
     2401 ctcggtagtg gaacaagtgc cgttgtggcg aggaaactgc aacgccgcta ttgcggtatc
     2461 gaattagact gggaatactg ttgttgggcg ctcaaacgac tggaagccgc tcgaacagat
     2521 gcggacattc agggctactc ggacggcgtt ttttgggaga ggaatggtat ttcaacgttt
     2581 aagagtgtgg tgccaataag gatcccaatg atgatgaaaa cggtcaaagt caagccataa
     2641 agcagaatgt ccagccaggg ccggcgcaca gcaggcgcag ttactggctg cggtgcggca
     2701 accggttgag ggttgacggc aaca
//
LOCUS       DAOQUC010000442         2723 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_44402_length_2723_cov_2.842579, whole genome shotgun sequence.
ACCESSION   DAOQUC010000442 DAOQUC010000000
VERSION     DAOQUC010000442.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2723
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44402_length_2723_cov_2.842579"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2712
                     /locus_tag="PKH07_08525"
     CDS             <1..2712
                     /locus_tag="PKH07_08525"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNT35027.1"
                     /translation="ITNSPPLPIPYSLSVMLGQRMLPFLALLLCVAFLQLIPTVENRE
                     RQDSRALQMRLTRFIMIFVLLLLTLHPGKNIDLSSHLALRCLGSLLILLGLGQRIWTS
                     GSVQLERVLYSLIFKSSHLVFLSTLSFCFILAALLFCGLVLGFLPHVADSMSLLWQGK
                     IFAAGRLSIPALPREIQPFFVFFYAITGDSLYSIYPPGQPLLFAIGCLLGAPYLVSPL
                     LGAGSLVGIYLVGRQIADDAIGRLSALLASFSPFLLFMSGSFMAHTGTLFFNTFGTYF
                     FLRLVKPKEGSSACSPSRRLSFLDASACGFCFGMAFLCRPYTTAAFFPVILAGSFWFL
                     IRNRWRNSTSFLLAGLIAAIFLVGFMFYNQKTTGDPLTTGYQVGSSMEQFSLQWSPAN
                     GVRNTATNFSALHLLLFAWPVPSLFAVWWLLQSRRMRRTDIYLLLMGLAPVAAYFFYS
                     FQDLCYGPRFFYTSSLFFIVLSARALMELRMQVRHICRGIHPLPVALTLVCFVFTVLR
                     IAPNMNSYWQASPERTVQKVRDAGITDGLVLVDRWDALPTTLNSISFDSRIVYALNRG
                     HRNLMFLHWYPDRDIYHVRPEGVTPVRILSQAEPGERLHNDLLREKLSNPTLAYQRLD
                     TDLDEEIMNRIISLEKTPFLWADMVLDNSDQVFIMDVWGRVYRRDPDRIRMLNTAPFK
                     GINRTLIATSFCLNPTDNSFTVLDQRGGIYQVSTMQREPEVLELPNLSVLTLQIEYGS
                     EGYFLVLCEDGSVLRWNPDSGYHTVSSSPETENVEYRMCVFDDGRAIVTMDTRGALYL
                     IGSAQTLVDVPLGKTWDWEAARAIRYLGTEPASFVTMDAFGAIYIRGEPGLDLFTENL
                     PYNTSWEYAVDIEIGKDSQTLYLLNRNGEVWLSRPQDHSREVSGSGF"
BASE COUNT          575 a          726 c          656 g          766 t
ORIGIN      
        1 atcacaaact ctccgccact ccccattcct tactccttga gtgtaatgct tggccagagg
       61 atgcttccat ttctggcact gcttctttgc gtcgcttttc tgcaactgat cccaactgtg
      121 gagaacagag aacgccaaga ttccagagcg ctccagatgc ggttgactcg tttcatcatg
      181 attttcgttc ttcttctcct aactcttcat cccggcaaga acattgacct ctcatcgcat
      241 ctagccctgc gatgcctcgg aagtctgctg attcttcttg ggttgggaca gagaatctgg
      301 accagtggca gtgttcaatt ggagagagtt ctctacagtc tcatattcaa gagcagtcac
      361 ttggtcttct tgagcacact ttccttctgc tttattctcg ccgcactgct cttctgcggt
      421 ctggttcttg gatttcttcc tcacgttgct gattcgatgt ctctactatg gcaggggaag
      481 atattcgcgg caggacgtct ctcaattcct gcactcccca gggagataca gccctttttc
      541 gtcttcttct atgctataac aggggatagc ctgtactcca tatacccccc gggacaaccc
      601 ttgcttttcg ctattggctg cctgctcgga gctccctatc ttgtcagccc cttactgggc
      661 gcagggtcct tggttggcat ttatcttgta ggaagacaga tcgccgatga cgcaatcggt
      721 cgtctttcgg cactgctggc ttcattcagt cctttccttc tattcatgtc ggggagtttc
      781 atggcgcata caggaactct cttcttcaac acttttggca cctacttctt ccttcgtctg
      841 gtcaagccga aagaaggttc ttcagcgtgc agtccaagcc gacgtctctc ttttttggat
      901 gcatcggcct gtggcttctg ttttggcatg gcctttctct gtcgtcccta cacaacggca
      961 gccttctttc cagtgatact agcgggcagt ttttggttcc tcatcaggaa tcgctggcgc
     1021 aacagcacat cgtttctctt ggctggactc attgcagcca tttttcttgt tggatttatg
     1081 ttctacaatc agaagaccac aggtgatccc ttgacgacag gatatcaggt cggaagcagc
     1141 atggagcagt tttcgctgca atggagtcct gcaaatggag tgcgaaacac cgcaacgaat
     1201 ttctcagcgc ttcatcttct gctgtttgca tggcctgtac cctctctttt tgctgtgtgg
     1261 tggctcctgc agtctcgtcg aatgagacgc actgatatct atctgcttct tatgggcctt
     1321 gccccagtgg cagcgtactt cttctacagc tttcaggatc tctgttatgg tccgcgattc
     1381 ttctacacat cctcactgtt ctttatcgtg ctttcggcac gcgcactgat ggagctcagg
     1441 atgcaagttc ggcacatatg tcgagggatt catccgcttc cagttgcttt gacattggtg
     1501 tgctttgttt tcacagtgct tcggatcgct cccaacatga acagttattg gcaggcttcc
     1561 cccgaaagaa cagttcaaaa ggttcgcgat gcagggatca cggatggtct tgttctggtt
     1621 gacagatggg acgctcttcc taccaccctc aatagtattt cgtttgatag cagaatcgtc
     1681 tatgctttga accggggtca caggaatctg atgtttcttc actggtatcc tgaccgtgat
     1741 atctatcatg tgagacccga gggtgtcaca cccgttcgga tcctgagtca agcagaacct
     1801 ggagagcgtc tgcacaacga cctacttcga gagaagctca gcaatccaac tttggcatat
     1861 caaaggctcg acaccgatct tgacgaagag atcatgaacc ggatcatttc gctcgagaag
     1921 actccgtttc tctgggcaga catggttctt gacaactcag atcaggtatt catcatggac
     1981 gtctggggac gagtctatag gcgcgatcca gaccgcatcc gaatgctcaa cacagctcca
     2041 ttcaagggca tcaatcggac actgatcgcg acaagctttt gcctgaaccc aacggacaac
     2101 tcgttcacag tgcttgatca gcgcggcggc atctaccagg tttccaccat gcagagagag
     2161 cctgaggttc tcgaattgcc caacctgtcc gtcttgacac ttcagatcga gtacggttcc
     2221 gaaggatatt tccttgtact ctgcgaggat gggtcagtcc ttcgatggaa tccggattca
     2281 ggatatcaca cagtctcttc atcgccagag acagagaatg ttgagtaccg aatgtgtgtc
     2341 tttgatgatg gcagggcgat agtgactatg gatacacgag gggcactcta cctgatcgga
     2401 tcagcacaga cattggtgga cgtacctctt ggcaaaactt gggactggga agctgcccga
     2461 gccattcggt atctcggcac ggagcctgcc tcctttgtca cgatggatgc ttttggcgcg
     2521 atctatatta gaggagagcc tggtctcgac ctcttcacag agaacctccc ctacaatacg
     2581 agctgggagt acgcagttga cattgagata ggcaaagaca gtcagaccct ctacctgctc
     2641 aacaggaacg gagaggtatg gttatccagg ccacaggatc atagcagaga agtctcaggc
     2701 tcaggattct gactttcgga cgg
//
LOCUS       DAOQUC010000443         2722 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_44419_length_2722_cov_2.966254, whole genome shotgun sequence.
ACCESSION   DAOQUC010000443 DAOQUC010000000
VERSION     DAOQUC010000443.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2722)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2722)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2722
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44419_length_2722_cov_2.966254"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..867)
                     /locus_tag="PKH07_08530"
     CDS             complement(<1..867)
                     /locus_tag="PKH07_08530"
                     /inference="COORDINATES: protein motif:HMM:NF037626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35028.1"
                     /translation="MNAPCVAAPLSFLYAEREGLNRVLERLPKPGEQATFGGLVSSSP
                     AWLVAFLAQQRPRPFLVIVRDQNRLYEMVEDLQFFFREALGETAVKVLPFPHVETLPY
                     ESKPPEMSIEVERAEVFKQLLAPSGQVENLAPMDEGGSSDEQRVIIVAPIRALQRRLP
                     PAWLVRDILWVLEVGSQVDRNSLVRWAEQEGYERRDLVAIPGHYSIRGGIMDLFAFTH
                     EIPIRIEFFGNEIESIRLFDPMTQRSVKQVKSVHLVPLGRENLVVQSWKSGQALDSLL
                     AYLPERTLVAVCE"
     gene            complement(988..2355)
                     /locus_tag="PKH07_08535"
     CDS             complement(988..2355)
                     /locus_tag="PKH07_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020614165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNT35029.1"
                     /translation="MSFRILVVEDDTNTREALRKALAVPGNEVITAQDGSSALAILDD
                     KVVDLVVSDIRMPRMDGMQLLTEIRGRFPSTHVILVTAYGKIESAVEAMKQGAFHYLT
                     KPINLDELDQLVSRVKSQHGLEIENEYLREEFEKRYGLRSLIAESPPMRNVFEIISRI
                     APSRSNVLITGESGTGKELVAYSIHLQSPRAKKPFVAVHCAALAENLLESELFGHERG
                     AFTGAVQQRKGRFEIADGGTLFLDEITEISPAIQVKLLRVLQERQFERVGGTDTLTVD
                     IRIVAATNRDIEKAVEEGRFRDDLYYRLKVVTIPVPPLRERKEDIPLLVDSFVTQFCE
                     ENNKRKMPLAQEAMRALCEHEWKGNVRELRNVIESMVVLARGETLTVADLPREIAREP
                     EPSNLVIPKFMPLADVERHHILKTLQDVEGNKSRAADLLGIGRRTLIRKLHEYQKNGL
                     YKETE"
     gene            complement(2500..>2722)
                     /locus_tag="PKH07_08540"
     CDS             complement(2500..>2722)
                     /locus_tag="PKH07_08540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35030.1"
                     /translation="WVPSLKPAHRATENAVGPCADRLLLGIPQSKEVGIGHTPAGVWP
                     ARNDKCATVLVFFWLGGEISSAGGRRGHR"
BASE COUNT          631 a          778 c          706 g          607 t
ORIGIN      
        1 ctcacagaca gcaacaaggg ttcgctccgg cagataggca agcaggctgt ccaatgcctg
       61 ccctgacttc cagctctgaa cgacaagatt ttccctgccg agaggaacca ggtgaaccga
      121 tttgacctgt ttcaccgagc gctgcgtcat tggatcgaac aatcggatgg attcaatttc
      181 gttgccaaag aattcgatgc gaatcggaat ctcatgcgta aaggcaaaga gatccatgat
      241 cccgccacga atcgagtaat gtcctggaat agcgacgaga tctcgcctct cgtagccttc
      301 ctgctccgcc catcggacaa ggctgtttcg atcaacctga ctgcccactt ccaacaccca
      361 gaggatgtcc cgaaccagcc atgcaggggg aagacgccgt tggagtgcac ggatgggagc
      421 aacgatgatc accctctgct cgtcggaaga acctccctca tccatcgggg caagattctc
      481 cacctgtcct gaaggggcga gcaactgctt gaacacttca gcacgctcaa cttcaatgga
      541 catctcgggt ggcttgctct catagggaag tgtctcgaca tgcggaaaag gcagcacctt
      601 gacagctgtc tcacccaagg cttcacggaa gaagaactgg agatcctcaa ccatttcata
      661 gagacggttc tgatcgcgaa cgatcacaag aaacggtctg ggtcgttgct gcgccagaaa
      721 ggcgaccagc cacgcgggcg aggaactgac aaggccgcca aatgtcgcct gctcccctgg
      781 cttggggagt cgctcgagaa cgcgattgag accttctctt tctgcgtaca gaaaagacaa
      841 cggcgctgcc acgcaaggag cattcatcag acccattgct cctttgagtg agtctgcagc
      901 gatgtccagc cgtcgtggag agagactcca gtcggatgat tctcaggaag aactactgga
      961 aataaaaagg cgggtcgcac ggtggtttta ctctgtttct ttgtagagac cgttcttctg
     1021 gtactcatga agctttcgga tcaatgttct tctgccaata ccgagaagat cggctgcacg
     1081 gctcttgttg ccttcaacat cctgaagcgt cttcagaatg tgatgccgtt cgacatccgc
     1141 gagcggcatg aatttcggaa tcaccagatt cgagggttcc ggctctcgag ctatttctct
     1201 gggtaggtcg gcaacggtca gtgtttcgcc tcgggcgaga acgaccatac tctcgatgac
     1261 gtttcgcagc tcccgcacgt tgcccttcca ttcatgttcg caaagcgccc tcatggcttc
     1321 ttgtgcaaga ggcatcttgc gcttgttgtt ctcctcacaa aactgcgtca cgaagctgtc
     1381 caccaggagt ggaatgtctt ccttgcgctc acgaagaggc ggaacaggaa tggtcacgac
     1441 cttcaaccga tagtaaagat catctcgaaa acgaccttcc tcaaccgctt tctcgatgtc
     1501 tcgattggtg gccgcgacaa tgcgaatgtc caccgtcagt gtatcggttc cgccaacacg
     1561 ctcgaactgc cgctcctgaa gtacgcgaag aagtttgacc tgaatcgcgg ggctgatttc
     1621 ggtgatttca tcaagaaaca gggttccacc gtccgcgatt tcaaagcgtc ctttgcgctg
     1681 ctgaaccgca ccggtaaatg ctcccctctc atggccaaag agttcactct ccagcaggtt
     1741 ttccgccaaa gcggcgcagt gtaccgccac aaaaggcttc ttggctcggg ggctttggag
     1801 atgtatggag taggcaacaa gttctttgcc ggtgccggac tctcctgtta tcaggacgtt
     1861 cgaacgggaa ggtgcgatgc ggctgatgat ctcgaaaaca tttcgcattg ggggactctc
     1921 agcgatgaga gacctgagcc cgtagcgctt ttcaaactcc tcgcgaaggt attcattctc
     1981 aatttcgaga ccatgttggg atttcacccg actgacgagc tgatccagtt catcaaggtt
     2041 gatcggtttg gtcaggtaat gaaaagcgcc ctgtttcatc gcctcgactg ccgactcgat
     2101 cttgccgtag gcggtgacaa gaatgacgtg cgtggaagga aaccggccac ggatttcggt
     2161 caacaactgc ataccgtcca tccgaggcat acgaatatcg ctgacgacga gatccaccac
     2221 tttgtcgtcc agaatcgcca gcgccgaact cccatcctgc gcggtgatga cctcgtttcc
     2281 cggcactgcc aacgccttgc gaagcgcctc acgggtgttg gtgtcgtctt ccacaacaag
     2341 aattcgaaaa ctcaaccgca gtcacctctg catctcggta ttgctgtcgt gctactgctg
     2401 cccgctcaac acatcctaca tctaccacaa agcaggaacg gaatccaatg ggagccgtag
     2461 gatagatcgc cagacccaaa cgttgtcttt tgactctctt taccggtgac cacgccgacc
     2521 acctgctgaa gagatttcac caccaagcca gaaaaacacc aagactgtgg cacatttgtc
     2581 atttcgagca ggccacacgc ctgcaggcgt gtggcctatg cccacttcct tggactgagg
     2641 tatccccagg agcagacggt cggcacacgg gccaaccgcg ttctccgtcg ctcgatgggc
     2701 aggtttgagt gagggaaccc ag
//
LOCUS       DAOQUC010000444         2718 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_44511_length_2718_cov_3.276756, whole genome shotgun sequence.
ACCESSION   DAOQUC010000444 DAOQUC010000000
VERSION     DAOQUC010000444.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2718
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44511_length_2718_cov_3.276756"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..397
                     /locus_tag="PKH07_08545"
     CDS             <1..397
                     /locus_tag="PKH07_08545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35031.1"
                     /translation="IERDIMAAQERIKSPVLLNLTADYGNVNSDDIYPKRLPDLFLND
                     AIILYGLYDKQQSCALRIVGETGQEKREFLFDIPLPKEDTGPEDIARYWALQKIYELI
                     GRMSRTGEEPGLQSQVDEISRQFKLKVPY"
     gene            421..1377
                     /locus_tag="PKH07_08550"
     CDS             421..1377
                     /locus_tag="PKH07_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35032.1"
                     /translation="MDKLDFTRIKTVPIASRESKVRLEDLAQVPRPGASFAEFFNSLP
                     SILKVQDLRLLVAKIAEAVRDNKPRIVLFGAHLIKCGLSPYLVEGMKRGIWTTLATNG
                     AGSIHDFEIAIQGATSEDVGKGLADGTFGMAEETGRWMNEAIREGAESGLGFGEALAR
                     KMESLQLPYRHHSVVYQAMQTNVPMCVHVALGTDIIHQHPEASGSAIGETSFRDFRKL
                     TECVAELKGGGVVLNLGSAVILPEVFLKALTVARNLVDGVHGFTTANFDMLQTYRPHH
                     NVVSRPTETGGIGLSFIGHHELLLPLFFYAVFEACKSKSQLF"
     gene            1461..>2718
                     /locus_tag="PKH07_08555"
     CDS             1461..>2718
                     /locus_tag="PKH07_08555"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HNT35033.1"
                     /translation="MQQVIVIVVDSLSAQQFEKQLALGHLPNIQRDFVEWGLWVRECV
                     ASFPPTSFSSHASIFTGTLPYRHGVASLRWFDRESGHHRSYAGFGAMLLNQDLLNGLG
                     TLHHSFVGEKNTAIGQVVNEGASSAVPPLIPSDGVRMKILRRQFSGKRPPALATIWLT
                     ELDPVAHKYGPESPQAERHLQQIDRMIGALMNDLHRRGLDEKTLCVFLADHGQVPCQQ
                     FESLPTFLKRTGFDVLDIFYYTLRDEFLFRRYDAVAWGCGVGVYFLYFPHRTGASRHL
                     NWKQRPSLNELHSYPTKGKTVDLVHALLDRESVGLVVVREETQAVRVFGKSGSCRIRW
                     ILDEHIPTMTRASFAVEIVEGEDPLDFARWQETAALMDGAFHPASDWFSATVRTEMPD
                     APVQLVQAMLSERAPDIMVLAAPKWEL"
BASE COUNT          615 a          718 c          748 g          637 t
ORIGIN      
        1 tatcgaacgc gacattatgg cggcacagga acgcatcaag tcccccgtgt tgctcaatct
       61 cacggcggac tacggaaatg tgaactcgga tgacatctat cccaagcgtc tgcccgatct
      121 cttcctcaac gatgcaatca tcctctacgg cctctacgac aagcaacaaa gctgcgcgct
      181 tcgcatcgtt ggtgaaacgg gtcaggaaaa acgcgaattt cttttcgaca ttcctctacc
      241 gaaagaggat accggtcccg aagacatcgc ccgctattgg gcgcttcaaa agatatacga
      301 gctaatcgga aggatgtccc gcacaggaga ggaacccgga ctccagagcc aggttgatga
      361 gatcagcagg cagttcaaat tgaaggttcc ctattgagtc atcctgaatg aaggtggggt
      421 atggacaaac tggatttcac ccgaatcaag accgtcccga tcgccagtcg ggaaagcaaa
      481 gttcgtctgg aagatctggc acaggttcct cgacctggtg cgagtttcgc cgagtttttc
      541 aacagccttc cctcgatcct caaagtccag gatctgcgac tgcttgtcgc caagatcgct
      601 gaggctgtcc gtgacaataa gcctcgaatc gtgctcttcg gagcgcacct gatcaagtgt
      661 ggcctgagcc catatctggt tgagggcatg aagcgcggca tctggacgac gcttgcgacc
      721 aacggagcgg gttcgattca cgatttcgag atcgcgattc agggcgcgac ttccgaagat
      781 gtcggcaagg gtctggcaga tggcaccttt ggcatggcgg aagaaacagg gcgatggatg
      841 aacgaggcaa tccgggaagg cgctgagtca ggcctgggat ttggggaagc ccttgctcga
      901 aagatggaat cactccaatt gccataccgc catcacagcg ttgtctatca agcgatgcaa
      961 accaacgttc caatgtgcgt tcatgttgcc ttgggtacgg acatcatcca tcagcatccc
     1021 gaggcgtcgg gcagcgccat tggcgagacg tcttttcgtg atttcagaaa actgaccgag
     1081 tgtgtcgccg agctcaaagg cggtggcgtt gttctcaatc tcggctcggc agtcattttg
     1141 ccggaagtct ttctcaaggc gttgactgtt gcccggaatc tcgtggacgg tgttcatgga
     1201 ttcactacgg cgaatttcga tatgcttcag acttaccggc ctcatcacaa tgtggtttcc
     1261 cgtccgacag aaactggtgg catcggtctg tctttcatcg ggcatcatga acttcttttg
     1321 cccctgtttt tctacgcggt ttttgaagca tgcaagtcca agtcacagtt gttttaacat
     1381 ccgttctctg tttcacgatc tgcgctcaag ggactgatag tcccgtctct tctgtggtga
     1441 cagagagatc ctctcaagat gtgcaacaag tcattgtcat cgtggtggac agtctgagcg
     1501 cgcaacagtt tgagaagcaa cttgctctgg ggcatctgcc gaacatacaa agagactttg
     1561 tggagtgggg actctgggtc agggaatgtg tggcgagttt tccgccgacg tcgttttcaa
     1621 gtcacgcctc gatcttcacc ggaacgctgc cgtatcgcca tggagtggca agtcttcgat
     1681 ggttcgacag ggagtcaggc caccatcgct cctatgctgg ctttggagcg atgctcctga
     1741 atcaggatct gctcaacggt ctgggaacgc tccatcactc gtttgttgga gagaagaaca
     1801 cggcgatcgg tcaagtggtc aatgaggggg catccagtgc cgtcccacct ttgatcccca
     1861 gtgacggcgt ccgaatgaag attctgcgaa ggcagttttc cggaaaaagg ccaccggcac
     1921 tggcgacaat ctggctcacc gaattggatc ctgtcgccca caaatacggc cccgaaagtc
     1981 cacaagccga aaggcacctg cagcagattg acaggatgat tggtgcattg atgaacgatc
     2041 ttcaccgccg tggtcttgat gagaagacac tgtgcgtatt cctggcagac cacggacaag
     2101 ttccctgcca acagttcgaa tcgctcccca catttctcaa aagaacgggt tttgatgttt
     2161 tggacatttt ctactacacg ctgcgcgatg agtttctgtt ccgacgctat gacgcagtgg
     2221 catggggttg tggcgtcggt gtctattttc tctacttccc ccaccgaaca ggagcgtccc
     2281 gacacctaaa ctggaaacag aggccaagtc tgaacgaact gcactcctat ccgacaaagg
     2341 gcaagacggt tgatcttgtc cacgctcttc ttgataggga atccgtcggg cttgtggtgg
     2401 ttcgggaaga gactcaagca gtgcgtgtct ttggcaagtc aggcagttgc agaatccgat
     2461 ggattctcga tgagcatatt ccaacgatga ctcgcgcctc gttcgccgtg gagattgtgg
     2521 aaggagaaga tcccctggac tttgcccgtt ggcaggaaac ggctgcactg atggacggag
     2581 cctttcaccc cgcgtccgac tggttctctg caacggtgcg gacagagatg cccgatgctc
     2641 cagttcaact cgtgcaagca atgctctccg aacgggctcc cgacataatg gttctcgcag
     2701 caccgaagtg ggaacttg
//
LOCUS       DAOQUC010000445         2711 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_44665_length_2711_cov_3.317018, whole genome shotgun sequence.
ACCESSION   DAOQUC010000445 DAOQUC010000000
VERSION     DAOQUC010000445.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2711)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2711)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2711
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44665_length_2711_cov_3.317018"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..916)
                     /locus_tag="PKH07_08560"
     CDS             complement(<1..916)
                     /locus_tag="PKH07_08560"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35034.1"
                     /translation="MQPEAVQAAQSLQSQFNWMSWMIILAYVVFTTWLGHKLSGRQAT
                     IKDFFLGGRKLPWWAVSGSVIATEISAMTLVGVPAFLWAETGNMAYMVLGIGSVISRV
                     IVGLYFVPAYYEREIYSPYEYIGQKLGTRAQRVTSSLFMIGGSLGQGTRVLLTAMVAH
                     VVTDLNIYTCIWLVGLVAVLWTYIGGIVTVIWTDVIQFFIFLFSALVTLYAVGAVLMQ
                     NGISVGEMINAAHSAGKFHWLNFSWDFRQNYTIWAAVIASSIGGLNAYGVDQMMVQRC
                     FCCKGPKEARKAIIWSSIGQLIMIICLFV"
     gene            complement(1143..1436)
                     /locus_tag="PKH07_08565"
     CDS             complement(1143..1436)
                     /locus_tag="PKH07_08565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35035.1"
                     /translation="MDNVLELANPFAQETTEEGNVERFILPPKVQAKQQQETQSISSK
                     LKAMDDALIRFKFSVQQYRDSVTPGNECIIQDIDHGIMDLFALLEILKMGHRM"
     gene            1789..2106
                     /locus_tag="PKH07_08570"
     CDS             1789..2106
                     /locus_tag="PKH07_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HNT35036.1"
                     /translation="MHNAAKTKQDVLDRLHETRERIAALGVERLGLFGSFVHDTQTAA
                     SDVDILVEFQPGGKSFDHFMDLCFLLEDVLGREVELVTKESLSPYIGPHILKEIAYVP
                     VAA"
     gene            2253..>2711
                     /locus_tag="PKH07_08575"
     CDS             2253..>2711
                     /locus_tag="PKH07_08575"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNT35037.1"
                     /translation="MKIAVAHLERADFPVIREWIDTKVFPIFREPIDDGQLEMLLTQS
                     KDGRLTDLGLKAVDEDTGQALGFVHVVLDWKNELGHIQQILIGSPELRRQGIGSVLMQ
                     HTLGVLFGDHRLHRVQLFVDEVNPSAIAFYKKMGFQVDGFMRESRKVGDTF"
BASE COUNT          659 a          736 c          709 g          607 t
ORIGIN      
        1 caacaaacag acagatgatc atgatcaatt gtccgatgct gctccagatg atcgccttgc
       61 gggcttcctt gggaccctta cagcagaaac agcgctgcac catcatctga tccacgccat
      121 acgcattcag tccaccgatg ctcgacgcga tcaccgccgc ccagatcgta tagttctgcc
      181 ggaaatccca ggagaaattc agccaatgga atttgcctgc gctgtgcgcc gcgttgatca
      241 tctccccaac ggagatgccg ttctgcatca gcaccgcacc gacggcgtag agcgtcacca
      301 gggcggagaa caggaagatg aagaactgga tgacatccgt ccagatgacc gtcacgatgc
      361 ccccgatata tgtccagaga accgccacca gacccaccag ccagatacat gtatagatgt
      421 tcagatcggt aacgacatgg gcaaccatcg ccgtcaggag aacgcgagtg ccctgaccca
      481 gcgagccacc gatcatgaag agcgaactgg tcactctctg cgcacgggtt ccaagtttct
      541 gaccgatgta ttcatagggg ctgtagatct ctcgttcgta ataggcgggc acaaagtaca
      601 gtccgacaat gacgcgactg atgaccgaac cgatccccag caccatgtaa gccatgttcc
      661 ctgtttctgc ccacagaaat gcgggaacac caaccaaagt catggcgctg atctcagtgg
      721 cgatcacgga acctgaaacc gcccaccagg gcagtttgcg tccaccgaga aagaaatcct
      781 tgatcgttgc ctgcctgcca ctgagcttgt ggccaagcca tgtggtaaag accacgtagg
      841 ccaggatgat catccaactc atccagttga actggctttg cagcgactgc gccgcttgaa
      901 cggcttctgg ttgcatcacg cgctccttcc ggaagtgtga attacggcct tcagcaaagc
      961 agtttctccc tctctcgtca aggcttttca agagacaaca gagatgggtc tagagtccat
     1021 agctcccaga tgctattttc cagacttctt cgaagtatgc tgtaggggca acgcgcgcct
     1081 cgctcccgct cctcaatccc tgtctcgtcc ggtcgaagag gctcccgtgc ccgctcgtcg
     1141 cgctacatcc tatgccccat tttgagtatc tcaagaagag cgaatagatc catgatacca
     1201 tggtcaatat cctggataat gcattcgttt ccaggagtca ctgagtccct gtattgctgg
     1261 acagagaact tgaaacgtat cagcgcatca tccatggctt tcagtttgga tgatattgac
     1321 tgtgtttctt gttgctgttt cgcctgtact ttgggaggca gaatgaatct ctcaacatta
     1381 ccttcttccg tcgtctcttg cgcaaacgga ttggccaatt cgagtacgtt gtccatcttt
     1441 gttctccttt ctgtttacac accacacgca tcaaggcttg accagaacca tcttggctgc
     1501 agaggcatta ttgacgaaag gcaggacaga atctgtcaca tctgctgaga ttccttgcca
     1561 tcaagggcat tacttacacg gcgacgatgg ccgcctcagg atcggcactg gagggcaagc
     1621 gtgccatcgc caacaatccc gtgaccgtca aatcccttta ggaataccag gtggaaacgc
     1681 accttatgcc ggagtgaagc gggaggggat gcttgacggc aagcccaaga tggtgtctaa
     1741 tcttctcagt gttcgtcgca tccgttgccc ccgacgagga ctggtaccat gcacaatgca
     1801 gccaaaacaa aacaagacgt gttggacagg cttcatgaga cccgggagcg gattgccgct
     1861 ctgggagtgg agcgattggg gctgtttggt tcctttgtac atgacacgca gacagcagcc
     1921 agtgacgtag acattcttgt cgagttccag cccgggggaa aatcatttga tcatttcatg
     1981 gatctctgtt tcctgctgga agatgtcctc ggacgcgaag tcgaactggt gacaaaagaa
     2041 tccctcagcc cctatatcgg gccgcatatc ctgaaagaga ttgcctatgt ccctgtcgcc
     2101 gcttgaatac atcaagcata tgttggagga atgcgatttt ctctgccgag aggcgttggt
     2161 acagaccacc tgagtcgaga gttcggaaat actgtgggaa gaaggtctag ggcaaacggt
     2221 ggtttgcgct cggcagccaa aaaaggaaga gcatgaagat agccgtagca catctggaga
     2281 gagcggattt cccggtcata cgtgaatgga tcgacacgaa ggtattccca atcttccgag
     2341 aaccgatcga cgacgggcag ctcgagatgc ttctcacgca aagcaaggac ggcaggctca
     2401 ctgacttagg cctgaaagcg gttgacgaag atactggtca ggcgctcgga ttcgttcacg
     2461 tcgtgctgga ctggaaaaac gagctgggac acattcagca gatcctcatc ggaagcccag
     2521 agttgcgccg acaagggatc ggatcggttc tgatgcaaca cacgctcgga gttctattcg
     2581 gcgaccatcg gctgcatcga gttcagctgt tcgtggatga ggtgaatccc tccgctatcg
     2641 ctttctacaa gaagatgggt tttcaggtgg acggattcat gagggagtcc agaaaagtcg
     2701 gcgatacctt c
//
LOCUS       DAOQUC010000446         2702 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_44891_length_2702_cov_3.123158, whole genome shotgun sequence.
ACCESSION   DAOQUC010000446 DAOQUC010000000
VERSION     DAOQUC010000446.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2702
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44891_length_2702_cov_3.123158"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2702
                     /locus_tag="PKH07_08580"
     CDS             <1..>2702
                     /locus_tag="PKH07_08580"
                     /inference="COORDINATES: protein motif:HMM:NF019912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35038.1"
                     /translation="QIVAFNIYDEPTLEMARNALILSRAVEDADLRHRPAINVLWNPR
                     TLNRIYQELPPRAVFTDWYPLGANTPVGMYGQLESNIEAWGRTAFARGVPFWMVLQGL
                     NIPSSLRFPTTAECRYMAYLSLANNAQGILYYVYEPSGRGLVRQDFTPRPHYEAIGRM
                     YARLIRHESLLVSLDPSAPLVSCAPPGYTKTFKDDSDQCFAFVVNRDVKTERSIPVSC
                     PASVTQQFIVHDVFRNTVVPHRITSTDQGLLAEFYVGLAPGAGTLLQIQGVGRETLPL
                     SDVPLYDKSEGASVAVAEPCGMTEKETLGRQNTERGVSPAHTIECTNREQLQRTSLPD
                     IPGALAALQFPNVPEVFGFALTAQAAYIAAGESGLISVHLSPLSVVDATTEGKAFDVV
                     LSEDILSIAAGDEGARLYRVLEDSSLERQSVISGSPVFVAAIEDSLMALSSGLDEISF
                     WNVSQPEYPKFVSECSVGGCIHQIVFSNQNAFVALGDDGLVVLDLSKPDKPIERLRIG
                     GSVTRIGFSDHVLLACGSSGLCGFQITEQPFAATKVWSIPQLQYGSISASGNQAVHAA
                     GNLTYLNKVESGTPEITACLGLLSSARSVAGRNGNTFVLGDDMGFRVFDDRSRKAPAA
                     VASKPSTAPRGITTDGTHLFLVDARDGLIILDTAQDGSWRAVGSLKCPYPVDSVSVSG
                     SVAYLSSKTAGFFVADISNPADPQLIGMDTERTGNAVRTMASDNLLFVASQRFGLKIY
                     DRTDPSQPLFLSQLASGFSGNDLTLLGERVYLAAQGDYSTGQGLVAVDVGIPAKPQVL
                     EVSYTVNPLSICTVGELLAVADDAEGVLIFDTNGSSLKLVHRFDDLEAVKVVSHGSWM
                     AILTRTSGWWLARIQQDLRSEIYGAFGLTTEIPLDACFHDERLW"
BASE COUNT          580 a          752 c          728 g          642 t
ORIGIN      
        1 acagatcgtg gcattcaaca tctatgatga gccgacactg gagatggctc gaaatgccct
       61 catcctcagc cgagccgttg aggatgccga cctgcggcac cgacctgcga tcaatgttct
      121 ttggaatccc aggacgctca atcgcatcta ccaggaactg ccgccacgag cggtcttcac
      181 ggactggtac cctcttggtg ccaacactcc tgtgggcatg tatggccaac tggaaagcaa
      241 catcgaggca tggggacgaa ccgccttcgc gagaggtgtt ccattttgga tggtcttaca
      301 gggactcaac attccatcct ctctccgctt cccaacaacg gcggaatgcc gctatatggc
      361 ctacctctcg ctggccaaca acgcacaagg cattctctac tacgtctatg agccaagcgg
      421 gcgcggtttg gtgcgtcagg acttcacgcc acgcccccat tacgaggcca ttggtcggat
      481 gtatgcccgg cttattcggc acgagtcact gctcgtttca ctagaccctt cagcgccgct
      541 tgtctcgtgt gccccgcctg gatatacaaa gaccttcaag gacgatagtg accagtgttt
      601 cgcattcgtt gtgaaccgag acgtcaaaac agaaaggtca atccccgtct cctgccccgc
      661 cagcgttaca cagcaattca ttgtccatga cgtgtttcgc aatacagtcg ttccccatcg
      721 aatcaccagc accgatcaag ggttgctcgc ggagttctat gttggtctgg caccaggtgc
      781 aggaacactc ttgcagattc agggtgttgg acgcgaaact ctgcccctgt cggacgtacc
      841 cttgtatgac aaatccgaag gggcgagtgt cgcagtcgca gaaccctgtg gcatgaccga
      901 gaaagaaact ctcgggagac agaacacaga gcgcggggtc tcccctgcac acacgattga
      961 gtgcacaaat cgcgagcagt tacagagaac atctctgcct gatattccag gggcactcgc
     1021 tgcgctccag ttccccaacg ttccggaagt atttggattt gcgctgacag cacaggccgc
     1081 ctatatcgct gccggagagt ccgggttgat ctcggtgcac ctgagtccac tttccgtggt
     1141 ggacgcgaca acagaaggga aagcgtttga tgtggtgctg agcgaggaca ttctcagcat
     1201 tgccgccggt gacgagggag cgcggctcta ccgagttctg gaagacagtt ctcttgagcg
     1261 tcaatcggtt atctcaggct ctcctgtatt tgttgctgcg atcgaagact ccctcatggc
     1321 attgtcgtcc ggtttggacg agatttcatt ctggaatgtc agccagccag agtacccgaa
     1381 gtttgtctct gaatgcagcg ttggtggatg tattcatcag atcgtattca gcaaccagaa
     1441 cgcttttgtc gctctgggag atgacgggct tgtcgttctt gatctttcaa aaccagacaa
     1501 acccattgag cggcttcgta taggaggcag tgtcactcgg attggcttct ccgaccatgt
     1561 gcttctcgcc tgcggttcat cagggctttg tggttttcag atcacggaac aaccctttgc
     1621 agcgacaaag gtctggagca tcccccaact ccaatatgga agcatatctg cctcagggaa
     1681 tcaggcagtc catgcggcag gaaatctcac gtatctgaac aaggtggaat caggcactcc
     1741 ggaaatcacc gcttgccttg ggcttctgtc gtcagcgaga tccgtcgccg ggcggaatgg
     1801 aaataccttt gttctcggcg acgatatggg ctttcgggta ttcgatgacc gctcacgcaa
     1861 agcacctgca gctgttgcat ccaagccgtc aactgcaccc agaggaatca caacagatgg
     1921 aacgcatctg tttctcgtag atgccagaga cggcctgatc attctcgaca ccgcgcagga
     1981 tggctcctgg cgtgcagtag gatcgttgaa atgcccctat ccagttgatt ccgtgtcggt
     2041 gagcggctcg gttgcatatc tgtcgagcaa gactgcgggg ttcttcgtcg cggatatctc
     2101 caatcccgct gatccacagc tgatcggcat ggatacagag agaacaggga acgccgtccg
     2161 cacgatggct tctgacaatc tcctcttcgt cgcgtcacag cggttcgggc tgaagatcta
     2221 tgatcgaacc gatcccagtc agcctctgtt tctctcccag ttggcttcag gattttcagg
     2281 caacgatctg acccttcttg gcgagcgtgt ctatctggcg gcgcagggtg actattccac
     2341 cggtcaggga cttgttgctg tggatgtagg cattccggca aaaccacaag tccttgaagt
     2401 ctcctatacg gtcaacccgc tttcgatttg caccgttggc gaactgcttg ccgttgccga
     2461 tgacgctgag ggtgttctca tcttcgacac gaacggatca tcactcaaac tggtccatcg
     2521 cttcgatgat ctggaagcgg tgaaagtggt ctcccatggt tcctggatgg cgatcctgac
     2581 caggacatct ggatggtggt tggcaaggat tcagcaagac ctcagaagcg agatttacgg
     2641 tgcattcggt ctgaccactg agattcctct cgacgcttgt tttcatgatg agaggctctg
     2701 gg
//
LOCUS       DAOQUC010000447         2700 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_44930_length_2700_cov_3.286200, whole genome shotgun sequence.
ACCESSION   DAOQUC010000447 DAOQUC010000000
VERSION     DAOQUC010000447.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2700
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44930_length_2700_cov_3.286200"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..786
                     /locus_tag="PKH07_08585"
     CDS             <1..786
                     /locus_tag="PKH07_08585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35039.1"
                     /translation="RGPCHDAEHGHDVPTTAVDHVRAVYALCRELKRRCPKLIIECHD
                     PVWPWQTCIYVPTYFQQGFGERGAYDENWGFEYMWDCIQDLRSGKALALYYYNLGCNI
                     PLYLHITMAADNDACLFFWWAASTVRHLGIGGKLGHETVNPSGLPPHDPERRFAAYKR
                     QMHLYRQLKPYFVRGVFHGIGEHIHLHTLPGRSGGVMVVFNLTDGDQVIETCISHGLL
                     QASTLLPVKGAVATQENRGIKVTAEVPAMSPAVICIGDAVGAL"
     gene            897..>2700
                     /locus_tag="PKH07_08590"
     CDS             897..>2700
                     /locus_tag="PKH07_08590"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 20 zincin-like fold
                     domain-containing protein"
                     /protein_id="HNT35040.1"
                     /translation="MNTELALIPKPRNYSEDGGTLALKEDFKVWCTDDTALEAERLRE
                     FLAEHLDLRLSLMALEMEGFVVALSPRKPVIEQLLRRVDPEGYIIHVGRRELILRGGS
                     RTGIFWGEQTLRQMFSSGSRTVSSRIMDDPALSWRGLHLDLSGATPSFASLVLLLDQM
                     SQYKMNTVIMEYQDRLVYDGHPRLSRSGALTSSQLQRVLDFANDRHLQVIPLVPTVGR
                     TGYILRHQEYRKLGEKGSLQQVQLGNPAVLDLMKQILDQVLAVHPQCEFIHLGGDMCP
                     EAARAPEMGRKIRKKGQLQPFMKHIGDLCQHVLDKGVRPMIWTDVFWQKMDPFAVRAL
                     PKEVVLVDRNYAAEDVRCPFVFWGPQDIRLSRHAVETSQRFPRTSSLWFDGLSEAELG
                     FLEKYWQPDATWGQGEAFPLLKFFLDEGFEVLGAGAVKGVDDKAPFVPRHRVRLNNLC
                     TWAKVAERDGLLGVVATARSRDSLFAPVAEPYIGLTYTMLAAADFFWRGAHSDVEDYE
                     RRAGLNLYGDDEQLPNAIALLDEGIRQDDLLRLGLASEILSNIRENAVHEKRFVDYLA
                     LACDLLSWQKEFDAQLDEIGGRLHLADRGKMLPEE"
BASE COUNT          580 a          662 c          794 g          664 t
ORIGIN      
        1 cgcggcccct gccacgatgc ggaacatggt catgatgtac ccaccacggc agttgatcat
       61 gtccgcgccg tctatgctct gtgtcgtgaa ctgaagcggc ggtgtcccaa actcatcatc
      121 gagtgtcacg atcccgtctg gccatggcaa acatgtattt atgttccgac ctacttccaa
      181 cagggttttg gcgagcgcgg tgcgtacgac gagaactggg gcttcgagta tatgtgggac
      241 tgcatccaag acctgagaag tggcaaggcg ctcgcgctgt attactacaa cttggggtgc
      301 aacataccgt tatatctgca tatcactatg gcagccgaca atgatgcctg tctctttttc
      361 tggtgggcag cgtctacggt tcgccacttg gggattggcg gcaaactggg tcacgagaca
      421 gtgaatccca gtgggttgcc tccgcatgat ccggagagac gctttgcagc ctataagaga
      481 cagatgcacc tctatcggca actcaaaccg tactttgtgc gaggagtctt ccatgggatt
      541 ggagaacaca ttcatcttca cacacttcct ggccgttctg ggggtgtgat ggtggtcttc
      601 aatcttacgg atggggatca ggtcatcgag acgtgcatct ctcatggact gcttcaagcc
      661 agcacattgt tgcctgtcaa gggtgctgtc gcaactcaag agaatagagg gattaaggtc
      721 accgctgagg tgcctgcgat gtcgccagcc gttatatgca tcggggatgc cgtcggagcc
      781 ttgtaatccg tgtttcagtc aaggctggac gtttttgggg ttttctcttg aagaccaacc
      841 cgagatgtgc aaatatcctc aacaaatact gactaggggg cttgcgctta gttattatga
      901 atacagaact tgcgttgatt cccaagccgc ggaactattc cgaggatggg ggaactcttg
      961 ccctcaaaga ggatttcaag gtctggtgta ccgatgacac ggcattggag gccgagcggc
     1021 tccgagaatt tcttgccgaa cacctggatc tcaggctgag cctgatggcg cttgagatgg
     1081 aaggctttgt cgtcgcgctc agcccaagaa agcctgtgat cgagcagttg ctgcgccgtg
     1141 tggatcctga aggatacatt atccatgtcg gaagacgtga gctgatcctt cgtggtggca
     1201 gccgcaccgg gattttctgg ggtgaacaga cactgcgtca gatgttcagc tcgggcagca
     1261 gaactgtttc gtcacgaatc atggatgatc cggcgctgtc gtggcgagga ctgcatcttg
     1321 atctgagcgg tgctacgccg agttttgcct ctctggtgct gctgttggat cagatgtccc
     1381 agtacaagat gaacacggtt atcatggagt accaggatcg gctggtctac gacggacatc
     1441 caagactttc acgctccggt gctctgacct catcgcaact tcagagagtg ctcgattttg
     1501 ccaacgatcg acatcttcag gtgattcccc tggtgccgac agtgggaaga acagggtaca
     1561 tcctgcgtca tcaggaatat cggaagttgg gggagaaggg ctccctgcag caggttcagc
     1621 tcggcaatcc agcggtgctt gatctgatga aacaaattct ggatcaggtg cttgcggttc
     1681 atccccaatg tgaattcatc cacctgggtg gagacatgtg tcctgaagcc gctcgagcgc
     1741 ccgaaatggg aaggaagatc agaaagaaag gccagctcca gcctttcatg aaacatatcg
     1801 gtgacctgtg tcagcacgtt ctcgacaagg gtgtacgtcc catgatttgg acggatgttt
     1861 tctggcagaa gatggatcct tttgctgttc gagcattgcc caaggaagtt gttctggtgg
     1921 atcgaaacta tgctgccgag gatgtccgct gcccgtttgt attctggggt ccacaagata
     1981 tccgattgtc gaggcacgcg gttgagacaa gtcaacgatt cccgaggacc tcgtcgcttt
     2041 ggtttgatgg tctttccgaa gcggaactcg gttttttgga gaagtattgg caaccggatg
     2101 cgacatgggg acagggagag gcttttccgc tactcaagtt ctttctggat gagggttttg
     2161 aagtcctggg tgcaggagcg gtcaagggtg tggatgacaa ggcacccttt gtaccacggc
     2221 atcgcgtgcg tttgaacaac ctctgcacat gggcgaaggt tgccgaacga gatggacttc
     2281 tgggggttgt ggcaaccgcg cgttcgcggg actccctttt tgctccagtt gccgagcctt
     2341 atattggctt gacctacaca atgctcgccg cggcagactt cttctggcga ggtgcccatt
     2401 cggacgttga ggactacgag aggcgtgcag gcctgaatct ctatggtgac gatgaacaac
     2461 tgcccaacgc catcgcgctt cttgatgagg gcattcgtca ggatgacctg cttcgcttgg
     2521 ggttggcatc agaaatcctg agcaacattc gggaaaatgc cgtacatgag aagcggtttg
     2581 tcgactatct cgctcttgcc tgcgacttgc tcagttggca gaaggaattt gacgcccagc
     2641 tggatgagat tggtggcaga ctccatctgg ccgatcgggg aaagatgctt cccgaggaga
//
LOCUS       DAOQUC010000448         2698 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_44971_length_2698_cov_3.670450, whole genome shotgun sequence.
ACCESSION   DAOQUC010000448 DAOQUC010000000
VERSION     DAOQUC010000448.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2698
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44971_length_2698_cov_3.670450"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..982
                     /locus_tag="PKH07_08595"
     CDS             <1..982
                     /locus_tag="PKH07_08595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35041.1"
                     /translation="ALIKSRLDALSNGGNGSFAKQIGEHLKKIEIGQPVHSHSLSLFP
                     LLGSWEEKSRYVLLEEGLAAGTVAVTETSEAGQVPYLLLRNDGDLPVLLPEGAILKGE
                     KQNRVINTTILVAAKSVFTVPVSCVEQGRWHRSRHGFQADSHAPSSLRSKKMRAVQES
                     RAFRGTAESNQSEVWREVAFAMDSLNVRSNTGSMSDAYERRRVDLDVLRRNLTPPPQA
                     LGVVMCRNNRIVGMDLFDSSQTFSKVWPRLADGYLIEALNDAPCDQAVNASSVESFLS
                     DIPQFSRRADPTLGIGTEFNIQGNQMVGTGVLYEEAVCHLCAFAGSASGL"
     gene            complement(1074..>2698)
                     /locus_tag="PKH07_08600"
     CDS             complement(1074..>2698)
                     /locus_tag="PKH07_08600"
                     /inference="COORDINATES: protein motif:HMM:NF036826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1349 domain-containing protein"
                     /protein_id="HNT35042.1"
                     /translation="SNKYFIEGPYVIKYGSYYYMQYSGNAWNTPQYGVGYATATSQMG
                     PWTKSSENPFLQATSEVYGPGHGCFTQSPDRSEWFIVYHSLLTSTSWNRELNVDRVCF
                     FDAPSGPPRLGLFDGVTRRPQLFPFGAKVVPSGVNDEFEAPVDRNLWRIVNEDSARWN
                     IQDGSLTIQAQDGEVWLDRTDGKNIFLQYAPSGDFVAETKVFFKPQVNNEKAFLTLWY
                     DQNNYVSFGPMYFLGMGLPGPLLYSVFECNGVRTQNYVINPSPDSPVHVRIARTGTSF
                     QPSYSWDGETWSPLGTGYELNMSGPLQTGVGAFSPGVALGRVASFDSFRFLSAEWHLA
                     GVSDEFEESTLDRNLWTTHHEVSHAWTIQDGSVSITTAQGDFWRFRSDAQNLFLQRAP
                     TADFRIASQITITPLVNYEQAMIVVWQDHNNYLKLSTVYDNGLCLEAACETDGVYTKT
                     SIPNPFGSSLILAIERKEGHYFYQASGDGEDWIDVGTGCADPFVPDSGFALRVGFGAK
                     APESGAARIARFDWFRVTPSEGPSFLLDWTQY"
BASE COUNT          640 a          688 c          767 g          603 t
ORIGIN      
        1 tgcgctgatc aagtccagat tggatgctct gagcaatgga ggaaacgggt ctttcgccaa
       61 gcagatcggc gagcatttga agaagatcga gatcggacaa ccggttcaca gtcacagtct
      121 gagtttgttt cccctgttgg gttcgtggga agagaagtct cgttacgtac ttctcgagga
      181 gggacttgct gcggggacgg tggctgtgac ggagaccagt gaggcggggc aggtgcccta
      241 tcttctcttg aggaatgatg gggatctgcc cgtgcttctc cccgaagggg caatcctgaa
      301 gggcgagaaa cagaatcgag tgatcaacac gacgattctt gttgccgcca agagcgtgtt
      361 cactgttccc gtcagctgcg tcgagcaggg gcgttggcat cgcagcaggc atggttttca
      421 ggccgattca cacgctcctt ccagcctgcg gtcgaaaaag atgcgcgcag tacaggaaag
      481 tcgtgccttc cgcggcacgg cggagagcaa tcaaagtgaa gtctggcgag aggttgcctt
      541 cgcgatggac tcgctgaatg tgagatcgaa cacgggttcc atgagtgacg cctatgaaag
      601 acggcgcgtg gacttggacg ttctgaggag aaacctgaca cctccacccc aagcactggg
      661 agtggtcatg tgtcgaaaca atcgcatcgt cgggatggat ttgttcgact cttcgcagac
      721 tttctcgaaa gtatggcctc gtcttgccga tggatatctg atcgaggcgc tgaatgacgc
      781 accctgcgac caggcggtta atgcttcaag tgtggagtct ttcctgtctg atatcccgca
      841 attctccagg cgtgctgacc cgactctcgg cattggcacc gagttcaaca ttcagggcaa
      901 ccagatggtc ggtacggggg ttctgtatga ggaagcagtc tgccatctgt gcgccttcgc
      961 aggatctgcg tctgggttgt aggtggatag agggatcgtg cgtctgccct gtcacaactc
     1021 gatggggcag acgcgctttc tcatacgaca ggattcagat cagacaggcc gcgctagtat
     1081 tgcgtccagt cgagcaagaa cgatggccct tctgatggag tcacacggaa ccagtcaaaa
     1141 cgagcaatcc gcgcggcacc actctccggt gcttttgcac caaaaccaac tctcagagca
     1201 aatccggaat ccggaacaaa gggatctgca cagccggtgc ccacatcaat ccagtcttca
     1261 ccatcccccg aggcctgata gaagtaatgt ccctcttttc tttctatggc aagaatcaga
     1321 ctggagccga acggattcgg aatgcttgtc ttggtgtaaa ctccatcggt ctcgcaagcg
     1381 gcttccaggc acaagccgtt gtcgtagacg gttgagagtt tcaggtagtt gttgtgatcc
     1441 tgccaaacga cgatcatggc ttgttcgtag tttaccaaag gtgtgatggt gatttgagag
     1501 gcgatgcgaa aatcagccgt aggtgcgcgc tgcaggaaga gattctgcgc gtcggagcga
     1561 aaacgccaga aatcgccctg tgccgttgtg atggagaccg atccatcctg gatagtccaa
     1621 gcgtgggaca cttcatgatg agttgtccag agatttcggt cgagcgtgct ttcctcaaac
     1681 tcgtcactga ctcctgccag gtgccattca gcggacagaa atcgaaaaga gtcgaaagat
     1741 gcaacacgac ccaacgcgac acccggcgag aaggcgccaa cacccgtctg aagcggaccc
     1801 gacatattga gttcgtatcc tgttcccaag ggagaccacg tttcaccatc ccagctgtat
     1861 gagggttgaa acgaagtgcc cgttctcgca attcggacgt gaacgggact atcgggtgag
     1921 gggttgatca cgtaattctg tgttctgacc ccgttgcatt caaaaactga atagagcagg
     1981 ggacctggca gacccatccc caaaaaatac atggggccga aactgacgta gttgttctga
     2041 tcgtaccaca atgtcagaaa tgccttttca ttgttgactt gcggtttgaa gaagaccttg
     2101 gtctccgcaa cgaaatctcc ggaaggcgca tactggagaa agatgttttt cccatcggtt
     2161 cggtcaagcc agacttcgcc atcctgtgcc tggatggtga ggcttccgtc ctggatattc
     2221 catcgagcgg aatcctcatt gacaattcgc cacaggttcc gatcaacagg agcctcaaac
     2281 tcatcgttca caccagaggg cacaactttg gctccgaagg gaaacaactg aggtcgtctt
     2341 gtgaccccat caaaaagccc caaccgaggg gggccggatg gagcatcaaa aaagcaaact
     2401 cgatcaacat tcagttcccg attccaggaa gtgcttgtca ggagagagtg atagacgatg
     2461 aaccattccg aacggtcggg tgactgagtg aaacagccgt gtccggggcc atagacttcg
     2521 cttgttgcct gcagaaaagg gttctcggaa gacttcgtcc aaggtcccat ctgggatgtg
     2581 gccgtggcat agccaacgcc atactgaggg gtattccatg cattgcccga gtactgcatg
     2641 taatagtagc tcccgtactt gatgacataa ggaccttcga tgaagtactt gttgctgg
//
LOCUS       DAOQUC010000449         2695 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_45069_length_2695_cov_3.221212, whole genome shotgun sequence.
ACCESSION   DAOQUC010000449 DAOQUC010000000
VERSION     DAOQUC010000449.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2695
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45069_length_2695_cov_3.221212"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..411)
                     /locus_tag="PKH07_08605"
     CDS             complement(<1..411)
                     /locus_tag="PKH07_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004573261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HNT35043.1"
                     /translation="MHGKHVRLAHGGGGRLTGDLVREVFLKAFHNPTLNLLADAAPIQ
                     IARGQAIAFTTDSFVVSPPFFAGGDIGRLAIAGTVNDLAVSGSIPRYIACSFILEEGF
                     PMEHLERIVQSMAATANEAGVEIVTGDTKTVEKGR"
     gene            499..648
                     /locus_tag="PKH07_08610"
     CDS             499..648
                     /locus_tag="PKH07_08610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35044.1"
                     /translation="MLRLQRLCDAAEAGTAKQRASVALLRRLRFASEAYPGFITSTTE
                     PYPLP"
     gene            818..1339
                     /locus_tag="PKH07_08615"
     CDS             818..1339
                     /locus_tag="PKH07_08615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35045.1"
                     /translation="MYGSEARRQPTPTLVYAMGLLLFFLGAMGFFSNLQWVLLSLQRS
                     LPEGVLSQDLARHLEMLGTLEEQIPLFTFWVYYGLVLHSLFVFASYGLLRFSDFARRF
                     MQILLATDALAFLSLVVYYQMTKHVPRIVEHFHMRFVVAASRIVFLIFLGSPAFMESF
                     RTKGKRPKDNTVA"
     gene            1360..>2695
                     /gene="ligA"
                     /locus_tag="PKH07_08620"
     CDS             1360..>2695
                     /gene="ligA"
                     /locus_tag="PKH07_08620"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HNT35046.1"
                     /translation="MDIERRIQGLRNEIRKHDRLYHVENRPIISDQEYDQLYRELVDL
                     EQQHPEFLTPDSPTQRVGGTPLDGFRSERHRIPMLSISNTYSPGEVREFDARIHRMLQ
                     TADYDSALGISEQEFRNACGKPVEYVVEPKVDGVAIALCYEQGTFVKALTRGDGVEGD
                     DVSANVRTMRTVPLHLESIPESLSHLEVRGEVYMERADFESYNAELLQQAKEPFANPR
                     NATAGSLKQLDPREAAKRPLKMFVHSLGYMEGIQFQTHAEALHALADFGLRVIAPFHV
                     LPDIAAVIALCDEFESKRYDLPFGIDGLVIKVNSRHQQAILGSTTKSPRWVIAYKFKA
                     EQATTLLSNITWQVGRTGALTPVAELEPVPLAGTVIRRATLHNYDEIERKDLRIGDRV
                     IIEKGGEVIPKVVLSLKETRTGIERVPERPTQCPSCGGVVHRLEDEVVYRCDN"
BASE COUNT          615 a          708 c          726 g          646 t
ORIGIN      
        1 gcgacctttc tcaacggtct tagtgtctcc ggtgacaatc tcaacacctg cttcattggc
       61 cgtggcggcc atagactgaa cgatcctctc cagatgctcc atcgggaaac cttcttccag
      121 gatgaaactg caggcgatat aacgcggaat ggagcccgaa acggcaaggt cattgacggt
      181 gcctgcgatg gcaaggcgtc cgatgtctcc gcctgcgaag aatggtggcg aaacgacaaa
      241 gctgtcggta gtaaaggcga tggcctgacc acgagcgatc tggatcgggg ctgcatcggc
      301 cagcaaattc aaagtcgggt tatgaaaggc ctttagaaaa acctcgcgga caagatcacc
      361 cgtgagtctc cctcctccac catgtgccaa acgaacatgc tttccgtgca ttgctcaaac
      421 tctccattca agaccataat ccaaacgaca cagccccttg ccgttcgcgt attggctcag
      481 tcatggaggg gtggaggtat gcttcggttg cagaggctct gcgacgctgc cgaagcggga
      541 acagccaaac agcgggcttc ggtagcgttg ctccgaagac tccgctttgc ttccgaagca
      601 tatcctggct ttatcaccag cacaactgag ccgtatccgt tgccttgatt atggcacaaa
      661 cccagatgac tttgtgctat tgtctttgtg ccactctgtg gtggctgaga cttgggctat
      721 gcccacgcca gcaaatgctg atctgtatgt gtagcgatga ggcgagacac atcggttgtc
      781 cggatatcct attgcgtgcc aggagtaatt gagttctatg tacggatcgg aagcaagaag
      841 acagccgaca cccacgctgg tctacgcgat gggattgctt ttgttctttc tcggagcaat
      901 ggggtttttc tccaatctcc aatgggttct tctgtcgctt cagagaagtc tgcccgaagg
      961 cgtcttgtcg caagaccttg cccgccatct tgagatgctc ggcacgctcg aggaacagat
     1021 tccgctcttt acattctggg tttactacgg tttggtgctt cattcgttgt ttgtatttgc
     1081 ctcttacgga ctgctccgat tcagtgattt tgcgcgtcgg tttatgcaga ttctgctcgc
     1141 aacagacgct ctggcgttcc tgagtcttgt ggtctactat cagatgacca agcacgttcc
     1201 cagaattgtg gaacactttc acatgaggtt cgttgtggcc gccagccgca tcgtgtttct
     1261 gatctttttg ggatctccgg cttttatgga gtcctttcgc accaagggaa agaggccaaa
     1321 ggacaacacg gtcgcctgag aagggagtcc tgaattcgga tggatatcga gagacgcatt
     1381 caagggttgc gtaatgagat ccgaaaacat gatcggctct accatgtcga gaaccgtccg
     1441 atcatctccg atcaggaata cgaccaactc tatcgagaac tggtggatct ggagcagcaa
     1501 catcccgaat tcttgacgcc agactctcca acacagcgtg tcggcggaac tcctttggat
     1561 ggattccgct cagagcgaca ccgcattccg atgctctcta tttccaacac ctattctcct
     1621 ggggaagttc gggaatttga tgccagaatt cacagaatgc tacagactgc agactacgat
     1681 tcggctctgg ggatctcgga gcaggagttc cgaaacgcct gtggcaagcc ggttgaatat
     1741 gtagttgagc caaaagtgga cggcgtcgcc atcgcactgt gctacgagca gggaactttt
     1801 gttaaggcgt tgacgcgggg agatggggtc gagggagatg atgtttcagc caacgtgcgg
     1861 acgatgcgca ctgtcccgct gcacctggaa tccatcccgg agtctttatc tcatctggaa
     1921 gtgcggggtg aagtctacat ggagcgtgct gactttgagt cctacaacgc agagctgctt
     1981 cagcaagcca aggagccttt cgcaaacccg agaaacgcca cggcaggctc attgaaacaa
     2041 cttgatcctc gtgaagcggc caagcgtccc ctgaagatgt ttgtgcattc actgggatac
     2101 atggaaggca tccaattcca aacacatgct gaagctctgc atgcccttgc ggacttcggc
     2161 cttcgtgtca ttgcaccgtt tcatgtgttg ccggacattg cggctgtcat tgcactctgt
     2221 gatgagtttg agagcaagcg ttacgatctg cccttcggca ttgacggctt ggtcatcaaa
     2281 gtcaactcac gacaccagca agcgattctt ggcagcacga ccaagagccc gcgttgggtc
     2341 atcgcctaca agttcaaagc agaacaggca acaactttgt tgagcaacat cacttggcag
     2401 gtgggtcgga caggagcatt gacaccggtt gccgagttgg aaccggtgcc tttggcaggt
     2461 actgtcatcc gacgtgcaac tctgcacaac tatgatgaga tcgagcgtaa ggatttgcgc
     2521 atcggcgatc gtgtgatcat tgaaaaaggc ggggaagtca tccccaaagt ggtcctgtct
     2581 ctcaaagaga cacgcaccgg tatcgaacgc gttccagaac gaccaactca atgcccttcc
     2641 tgcggcggag tcgttcatcg tctcgaagac gaagtcgtct atcgctgcga taatc
//
LOCUS       DAOQUC010000450         2693 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_45117_length_2693_cov_3.144428, whole genome shotgun sequence.
ACCESSION   DAOQUC010000450 DAOQUC010000000
VERSION     DAOQUC010000450.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2693
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45117_length_2693_cov_3.144428"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="PKH07_08625"
     CDS             <1..345
                     /locus_tag="PKH07_08625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35047.1"
                     /translation="DGDWTWPRQEGLALLALIRQVAASANALPSVRMEKFSPSVEIVV
                     RQTEDDQRIFLIHTAQGMRCNDRLVLSSSPASEPKVWADFEERPVKIETNDSGESVLV
                     LPAFSDCCTVQW"
     gene            381..1820
                     /locus_tag="PKH07_08630"
     CDS             381..1820
                     /locus_tag="PKH07_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNT35048.1"
                     /translation="MNRREFVKVLGWGACVAVAKSGWAMGRKVPSKPNVILCMADDQG
                     WGDMGYNGHPVLKTPNFDAMASEGVRFDHFYSAAPVCSPTRGSCMTGRHPNRFGCFSW
                     GYPLRPQEITVAKALKSAGYATGHFGKWHLGSVCKGSPVNPGASGFDEWFSSPNFFDV
                     DAVLSREGTAVQTHGESSMVTVDAALEFIRDKAEHKQPFLAVVWFGSPHDPYVALDED
                     KALYPDERPQLRSYYGEITAMDRAFGKLRNELGSLGIRDNTILWYCSDNGGVSKVSVT
                     GGRKGKGSIYEGGLRVPALLEWPAAFPEPFVTDLPSCTSDFYPSLLDSAGVRIKGQPP
                     LDGISLLPLLRKGSAKRPRPMGFWDYAAKGIRTPSKEWMAEMLQKQMAGQDETDPTRL
                     LMDAAVIEKQYSEEDYPGHAAWNDGNWKLHRITDKSGSITWELYNLREDPEEQRDVLP
                     NNARRVKRMQRELHQWLASVTRSLNGKDY"
     gene            complement(1814..>2693)
                     /locus_tag="PKH07_08635"
     CDS             complement(1814..>2693)
                     /locus_tag="PKH07_08635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35049.1"
                     /translation="RITIKAVDLGGWPVGTVFLPATLFVIVSKRVSGVLRRILLLGLC
                     LFLFWAYTYRDTRFLLLPLAFLSLGIGICADPILRKRGLWGQSVAWVFAIGMLVNYAS
                     FAHSLFSRHFPGEYLTNTIGREGFLAKRLDYYPAFMELEKLSDDNISDSDKVLFIGEH
                     RGMYCPVDYVASDWFDTPVIVRWLRETRDNLELLERLRAEGIGYVLYNEKELSLYFSP
                     WFLLQFLPEAQALQCLETARNQGRNLNSVEERQFLERQATMSPLFERYKQFIENRQWL
                     EPIWEEQGVTLFRVLQ"
BASE COUNT          638 a          673 c          787 g          595 t
ORIGIN      
        1 gatggcgact ggacttggcc cagacaggag ggacttgcac tgcttgccct tattcgacaa
       61 gtggctgctt ctgcaaacgc acttccttcc gttcgcatgg agaagttttc cccttcggtc
      121 gaaatagtcg tgaggcagac ggaagacgat caacgcatct tcttgattca taccgctcag
      181 gggatgcgct gcaatgaccg cttggtgctt tcctcttcac cggcgtcgga accgaaagtc
      241 tgggctgatt tcgaagagcg tccggtgaag atcgagacca atgactcagg ggagtccgtt
      301 ctggtgcttc ccgcgttcag tgactgctgt acggtgcaat ggtgaaatcc gcagtgagtt
      361 gacggagagg agaagacaag atgaatcgtc gtgagtttgt caaggtgctg ggctggggtg
      421 catgtgtggc tgttgcgaag tcgggttggg caatggggag aaaagtgccc agcaagccca
      481 atgtgattct ctgtatggct gacgaccaag gctggggaga tatgggctac aacggacatc
      541 ctgttctgaa gactcccaac tttgacgcca tggcctcaga aggagtccgg tttgatcatt
      601 tttactctgc cgctcccgtc tgctctccga cacgtggcag ctgcatgaca ggacgtcatc
      661 cgaatcgttt tgggtgcttc agctggggct atccacttcg tcctcaggag atcacggttg
      721 ccaaagcgct caagagtgcg ggctatgcga cggggcactt tggcaaatgg catctcggtt
      781 cggtctgcaa aggaagccct gtgaatccgg gcgcaagtgg cttcgatgaa tggttttcat
      841 cgccgaactt ctttgacgta gacgcggttc tgagtcgcga agggactgca gttcagacgc
      901 atggcgagag ttccatggtt acggtggatg cagcactgga gtttattcgc gacaaggcag
      961 agcacaaaca gccctttctt gctgtcgtat ggtttggctc tccgcacgat ccctacgtgg
     1021 cacttgacga agacaaagcg ctttacccag acgaacgccc acaactccgt agctactatg
     1081 gtgagattac cgctatggat cgcgcttttg gaaagcttcg aaatgagctg gggagtttgg
     1141 gaatccgcga caatacgatt ctctggtact gcagtgacaa cggtggtgtc agcaaggtga
     1201 gtgtcactgg tggaagaaag gggaaaggtt cgatatacga aggcgggctc agggttcccg
     1261 cactgcttga atggcctgca gcatttccag aaccttttgt gaccgatttg ccttcctgta
     1321 ccagcgattt ctatccttcg cttttggaca gcgccggtgt gaggatcaaa ggtcagcccc
     1381 ccttggatgg aatcagtctc ctgccgttgc ttcgaaaggg cagcgccaag cgtcctcgac
     1441 cgatggggtt ctgggactat gctgcgaaag gcatcaggac tccctcgaag gaatggatgg
     1501 ccgagatgct gcaaaagcag atggctggcc aggatgaaac cgacccgacg cggcttctca
     1561 tggatgcagc ggtgatcgag aagcaatact ctgaagagga ttaccctggg cacgcggcat
     1621 ggaatgatgg aaactggaag ttgcatcgca tcacagacaa gtcgggcagc attacatggg
     1681 aattgtacaa tctcagagag gatcccgaag agcagagaga cgttcttcca aacaatgccc
     1741 gtcgggtgaa gcggatgcag cgcgaactgc atcagtggct tgcatcggtt acacgaagcc
     1801 tgaatgggaa ggactattga aggactcgga acagggtgac accttgttct tcccagatgg
     1861 gttcgagcca ctgcctgttc tcgatgaact gcttgtatcg ttcgaatagg ggactcatgg
     1921 tcgcctgccg ctcaagaaac tgtcgttcct cgacgctgtt gagattcctt ccctggttgc
     1981 gtgcggtctc caggcactgc agggcttgag cctcaggcag gaactgaagc agaaaccacg
     2041 gcgagaaata gaggctcaac tctttctcat tgtaaaggac atagccgatc ccttccgcgc
     2101 gcaggcgttc caacaactcc aagttgtccc gagtctctcg aagccagcgt actatcaccg
     2161 gagtgtcaaa ccaatcggat gcgacatagt ccactgggca gtacataccc cgatgctccc
     2221 cgatgaacag cactttgtcg ctgtccgaga tgttgtcatc ggaaagtttc tcgagttcca
     2281 tgaatgcagg atagtagtcc agtctcttgg cgaggaatcc ttctcgacca atggtgttgg
     2341 tgagatactc accagggaaa tggcggctga aaagagaatg cgcaaaggat gcgtaattga
     2401 caagcattcc aatcgcgaaa acccaggcaa cgctctgccc ccagaggccg cgttttctga
     2461 ggatgggatc cgcgcagatt ccgataccaa gactgagaaa agccaaggga agcaggagaa
     2521 atcgggtatc acgataggtg taagcccaga acaagaagag gcacagaccc agaagcagga
     2581 ttcttcgaag cacccccgat actctcttgg agacgattac aaagagagtt gctggcagga
     2641 agacggttcc aacaggccag ccaccaaggt caacggcttt gatcgtgatg cgc
//
LOCUS       DAOQUC010000451         2693 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_45124_length_2693_cov_2.923427, whole genome shotgun sequence.
ACCESSION   DAOQUC010000451 DAOQUC010000000
VERSION     DAOQUC010000451.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2693
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45124_length_2693_cov_2.923427"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..583
                     /locus_tag="PKH07_08640"
     CDS             <1..583
                     /locus_tag="PKH07_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870473.1"
                     /note="has hydroxylamine reductase activity, catalyzes the
                     reduction of hydroxylamine to ammonia and water; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HNT35050.1"
                     /translation="EAEGFTEDGPNREIMVGFGRQAVLSVADKVIEAVKSGAIRHFFL
                     VGGCDGAKSGRNYYTDLAKAIPKDCVVLTLACGKYRFNKLDFGDIGGIPRLLDIGQCN
                     DAYSAIQIAVALAGAFNTDVNSLPLSLILSWYEQKAVAILLTLLHLGIQNIRLGPSLP
                     AFVSPNVLNVLVDLFNIKPVGTVEQDLAEILGA"
     gene            704..1195
                     /locus_tag="PKH07_08645"
     CDS             704..1195
                     /locus_tag="PKH07_08645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35051.1"
                     /translation="MVIRQWITWVFVAFVFFTGSVAFGQEAVQAEKPQQEVKTQTTCP
                     VMGGTIDKNLYVDHEGKRIYLCCQGCADAVKKEPEKYIKKLEDEGITLDKTPKLQTKC
                     PMMNRPIDKKLYVDHQGKRIYLCCKGCVATVKKDPGKYIKQLEAEGVTLDKAPEEKKQ
                     KAE"
     gene            1293..2582
                     /locus_tag="PKH07_08650"
     CDS             1293..2582
                     /locus_tag="PKH07_08650"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HNT35052.1"
                     /translation="MSQWISQLLTETLEAGSWLSYIVLFGSGVITSFTPCTYPVLPLT
                     VGVIGGMAGGSRMRGLFLSVLLVLGMAFVYAVLGTVFAALGMQFGSLWGNGWVVYLIG
                     LFFIFMGLFLLDVFTFPSIAVFEGLRAKLDARKTGWIGVFAVGGVSALIVGPCTGPIL
                     AVVLGYITLSLRSTTGVAFGMQALNSGMKLLAFGLGQGALIVLCGTFAGFLAVLPRSG
                     AWLVQVKKAFALLIIVGASLLLVYVGQATDFPDLIGLLASLETTAPSEEQMPLDLDVS
                     AEEPATLDEDEAPELDDSFAPDFSLRVLDGSEFRLSQVRGSKAVVLVFFATWCPSCMA
                     EVPMINEFTEQIKDKDILVYGVNFRQKEDILRQFREKREVQYSILLDTDGVVARDYEV
                     TGIPLIVGVSKKGSVVYRDHHLPENRSAFVQSLLTGQ"
BASE COUNT          586 a          639 c          772 g          696 t
ORIGIN      
        1 cgaagcggaa ggattcaccg aggatggtcc gaatcgtgag atcatggttg gctttggacg
       61 gcaggcagtt ctcagtgttg cggacaaagt cattgaagcc gtcaagagtg gcgcgattcg
      121 ccatttcttt ctcgtcggcg gatgcgatgg agccaagagt ggtcgcaact actacaccga
      181 ccttgccaag gcgattccca aggactgcgt cgttctgaca ctggcctgtg gcaagtatcg
      241 cttcaacaag ttggattttg gtgacattgg gggcattcca cgccttctgg atatcgggca
      301 gtgcaatgac gcctattcgg cgattcagat cgccgttgca cttgcagggg ctttcaacac
      361 cgatgtcaac agccttccgt tgtcgctgat tctgtcttgg tacgaacaga aggcggtggc
      421 aattctattg acgctgctcc atctgggcat ccagaacatt cggcttggcc caagccttcc
      481 cgcttttgtc tctccgaacg tcttgaacgt cttggtggac ttgttcaaca tcaagccggt
      541 cggcacggtc gagcaagatc ttgcagagat tctgggtgcc tgagtcagga gctgacaaat
      601 cggcgcgagt ttgtgggaga cttgcatgac tcaagtctcc cccgactcgt tctgaaaaca
      661 cgttgagagt gtgatagatc tctttagaaa aggagtcatg aaaatggtta ttcggcaatg
      721 gatcacatgg gtcttcgttg catttgtgtt tttcacaggt tcggtggcct ttggacagga
      781 agccgtgcag gcggagaagc cgcagcaaga agtgaaaacc caaacgacgt gtcctgtcat
      841 gggtggaacc attgacaaga atctctatgt ggatcacgaa ggtaagcgta tctatctctg
      901 ctgccaggga tgcgctgatg ccgtgaagaa ggaacccgaa aagtacatca agaaacttga
      961 agatgagggg atcaccctgg acaagactcc gaaactgcag accaagtgtc ccatgatgaa
     1021 caggccaatc gacaagaaac tgtatgtaga ccatcagggg aaacgcatct acctctgctg
     1081 caaaggatgt gttgccacag tgaaaaagga tcctggcaag tacataaaac agcttgaggc
     1141 cgaaggcgtc acgcttgaca aggcaccgga agagaagaag cagaaggctg agtaacttct
     1201 ggtttgaggt ctatgctcaa caaatcaccg gggtgtggtt cggtctgcga accacaccct
     1261 gtgtgaacag atgacaatag gggtgttgca tcatgtcaca gtggatctct caactgctga
     1321 ccgagacgct cgaagcaggc tcgtggctgt cgtatatcgt cttgtttggg agcggagtta
     1381 ttacgagttt cacgccctgc acgtatcctg tcctgcctct cacggttgga gtcattggag
     1441 gcatggcagg ggggagtcgt atgcgtggtc tgttcctgag cgttctgctg gtattgggga
     1501 tggcctttgt ctatgctgtg cttggaacgg tgtttgccgc tttggggatg cagttcggtt
     1561 ccctctgggg caacggttgg gttgtctatc tgatcgggct gttcttcatc ttcatgggtc
     1621 tattccttct cgatgttttt accttcccaa gcatcgctgt ctttgaagga cttcgagcaa
     1681 aactggacgc ccgcaaaacg ggatggatag gcgtcttcgc cgttggtgga gtgagtgcac
     1741 ttattgttgg tccttgcacg ggaccgattc tcgcagtggt gctcggttac attacccttt
     1801 cacttcgttc cacgacgggg gttgcttttg gaatgcaggc gctcaacagc gggatgaaat
     1861 tgctcgcgtt cggtctggga cagggcgctt tgatcgtact ttgtggaacc ttcgccggtt
     1921 ttttggcggt gctccctaga tcgggggcat ggcttgtgca ggtgaagaaa gcctttgcac
     1981 ttctgatcat cgttggcgcc agccttctct tggtctatgt cgggcaggcg acagatttcc
     2041 ctgatctcat cggcttactt gcgtctttgg aaacgactgc accttctgag gagcagatgc
     2101 ctttagatct ggatgtttct gctgaagagc ctgcaacctt ggacgaagat gaagcacctg
     2161 aattggacga ctcctttgca cctgatttct ctctgagagt tctcgacggt tcagagtttc
     2221 gactcagtca ggtgcgtggc agcaaggctg tggtgctggt gttcttcgcg acgtggtgtc
     2281 cttcatgcat ggctgaagtc ccaatgatca atgagttcac ggaacagatc aaggataagg
     2341 acatactggt ctacggagtc aacttccgac agaaggaaga cattcttcga caatttcggg
     2401 aaaagaggga agtccagtat tccattctgt tggacaccga tggcgttgtc gcacgtgact
     2461 acgaggtgac aggaattccc ttaatcgtcg gggtcagcaa gaagggaagt gttgtctatc
     2521 gcgaccatca cttacccgag aaccgcagtg cctttgttca gagtcttctt acagggcagt
     2581 aggttttccg aagccagttt cccttacacc acgggtgtgc cgctgtgtgc tattccaatc
     2641 ggactgcggt tccgtagacc agaagttccg cggctccact catcatactg gat
//
LOCUS       DAOQUC010000452         2692 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_45155_length_2692_cov_3.075465, whole genome shotgun sequence.
ACCESSION   DAOQUC010000452 DAOQUC010000000
VERSION     DAOQUC010000452.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2692
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45155_length_2692_cov_3.075465"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..386
                     /locus_tag="PKH07_08655"
     CDS             <1..386
                     /locus_tag="PKH07_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HNT35053.1"
                     /translation="GRYEIQILDCYENLSYADGMTASVYGEFPPLVNACRKPGEWQTY
                     DIIWHGPRFEGDKLVKPARVTVLHNGVLVHHDTELFGPTTHRAIAPYTPQPTVGPLKL
                     QDHGDLVRFRNIWYRPVFDYDEASK"
     gene            complement(496..>1147)
                     /locus_tag="PKH07_08660"
     CDS             complement(496..>1147)
                     /locus_tag="PKH07_08660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35054.1"
                     /translation="VLLLLFLLGRSFFSAKTMPFSWHRLWVFLPGAVGVACPYLFRVF
                     AQDDNSGSITAMPLLIATVSPLIISTLRVRGRLLSTLLVLVGAMLTVGIALSIMEPSV
                     LASLWQILMISAMYGAVMGVHAVILCVVMHYRYTRKRLVIAYVLGSVLPTFFLIVLLM
                     WEAPKNFVMWVSVLFLLAPLPLTMLIALNSWVRAIMTGELFLKSERVEVPTAEPES"
     assembly_gap    1148..1157
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1278..2447)
                     /locus_tag="PKH07_08665"
     CDS             complement(1278..2447)
                     /locus_tag="PKH07_08665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35055.1"
                     /translation="MIKKIAVAVGLSLWVISACLADGGTLTFTDQGTPGRSKPAAGYN
                     WSTSTTVKASVRLPVAAPVADTTDSFVLSDEPQSVFVLSDMNVMVSEGSPRRVFSDLN
                     GDKECQAEEMGFSPRDNADEMRIPLNLGVTASDERIVDTTVYIYTSSRYCSLTVFAVA
                     GHYEGEIELAGNTYPARLDFANPMSKPSKGLVSLDCDADGEFASSIDLWFDPEFGVVF
                     DGVFYESSLTCRDATAEIRLVSTDESMGTLSLKGEGIVWASVRNALERPLFLPRRDSF
                     LYRMPVGDYKILACLLNDRSEPPNAFQLNVPSYDLPGFQIPADATHELALGGPLSQNI
                     VIESKPLLNLVFLDNKGLKNGAGFVYDLGERGAEPRYQIKNRRGDVVAKGKFEFG"
     gene            2500..2652
                     /locus_tag="PKH07_08670"
     CDS             2500..2652
                     /locus_tag="PKH07_08670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35056.1"
                     /translation="MQDGSLATQIVAITYWLSYARDEVNRHSEPFVGVYPEQRRRTQG
                     RLSLRA"
BASE COUNT          716 a          692 c          707 g          567 t
ORIGIN      
        1 tgggacgtta cgagattcag attctcgact gctacgagaa tctatcctat gcggacggga
       61 tgaccgcctc agtttatggt gagtttccgc cgttggtgaa tgcctgtcgc aaacccggtg
      121 agtggcaaac gtacgacatc atctggcatg gtccccgatt tgagggagac aaactggtga
      181 agccggcacg ggtgacggtt ctgcacaatg gcgtccttgt gcaccatgac accgagttgt
      241 tcggaccaac gacccacaga gcgatcgctc cctatactcc tcagccaacc gtgggaccac
      301 tgaaacttca ggatcacggt gacctggtgc ggttccggaa tatctggtat cggccagttt
      361 tcgactatga cgaagcctca aagtagcgga tcatcctaaa gagaccgatt ctgtttttag
      421 acgaaagaaa agccccagga actgtcaagt tctctggggc tttcggtatt gatatctttc
      481 agccaaatcg gcttgtcagg attctggttc agcggtcggt acttcgactc gttccgattt
      541 cagaaacagc tcacctgtca tgatggctct gacccaagag ttcagagcaa tgagcatggt
      601 aagcgggaga ggagcgagca ggaaaagaac cgacacccac atgacaaaat tctttggggc
      661 ttcccacatg agaagaacga tcaaaaagaa agttggaagc acagaaccca gaacataggc
      721 gatgacgaga cgctttctgg tgtatctgta gtgcatgaca acacaaagga tgacggcatg
      781 cactcccatt accgcaccgt acatcgcaga aatcatgagt atttgccaga gcgaggcaag
      841 cacgcttggc tccatgatgc tcaaggcaat gcccacagtc aacattgccc ccaccaacac
      901 cagcaacgtg gagagaagac gacctctcac tcgcagtgtg ctgatgatca gcggggagac
      961 agtggcaatc aggaggggca tcgccgtgat actgccagag ttgtcgtctt gcgcgaaaac
     1021 tctgaagagg tacggacaag ccactcctac agcacccgga agaaacaccc aaagccgatg
     1081 ccaggaaaaa ggcattgttt tcgcgctgaa gaagctcctg cctaggagga acaggagaag
     1141 aagaacannn nnnnnnngaa gtatgagaaa actgtactct ctgttttgac gaaatcggac
     1201 gccgtagcgt cagtcgcaac tgagtagttg ccataggaaa ggatgggcat tcgcacggaa
     1261 gcccgacatg tgccacctca accaaactcg aacttgcctt tcgcaacaac atcgcctcgg
     1321 cgattcttga tctggtatct cggctcagcg cctcgttctc ccaagtcata gacaaaaccg
     1381 gcgccgtttt tcagaccctt gttatcgaga aagaccagat tcagaagagg ctttgattcg
     1441 ataacgatat tctggctcaa cggtccacca agggcgagtt cgtgtgtcgc atccgcagga
     1501 atctgaaacc ctgggagatc gtaggacgga acgttaagct ggaacgcgtt ggggggctcg
     1561 gagcgatcgt tcaggagaca ggcgagaatc ttgtagtcgc caacaggcat tcgatagagg
     1621 aaactgtctc ggcgtggcag gaaaagcgga cgctccagag catttcgaac ggatgcccag
     1681 acaatgccct cgcctttgag ggaaagtgtg cccattgatt catcggttga aacaagacgg
     1741 atttcagcag tcgcatcacg acaagtcaat gaggactcat agaagacgcc gtcgaagaca
     1801 acaccaaatt ccggatcgaa ccagaggtca atgctcgacg caaattcccc atccgcatcg
     1861 cagtcaagtg agacaagacc tttactgggc ttggacattg gattggcaaa gtccaaccga
     1921 gctggatagg tgttgccagc cagttcgatc tccccttcat agtgccccgc aactgcgaaa
     1981 accgtcagac tgcagtagcg ggaactggtg tagatgtaga cggttgtatc aacaatgcgt
     2041 tcgtcgcttg cagtcactcc cagattcaag ggaattctca tctcgtcggc gttatcccta
     2101 ggggagaatc ccatttcctc agcctgacac tccttatcgc cattgagatc agaaaaaact
     2161 ctccggggac tgccttctga aaccatgacg ttcatatcag aaagcacaaa aacagattgt
     2221 ggttcatcag agagaacaaa ggagtccgtc gtatctgcaa ctggagcagc aacaggcaat
     2281 cggacagatg cctttacggt tgttgaagtt gaccagttgt atcccgccgc cggcttgctt
     2341 ctgccaggcg tcccctgatc ggtaaaagtc agagttccac catccgccag acacgccgag
     2401 atgacccaaa gggacagacc aacggcaact gctatcttct tgatcacgta tatgtcctca
     2461 tattcaggag tttgtttagc gaacgagagt gaggttagca tgcaagacgg gtcgctggca
     2521 acgcaaatcg ttgcgatcac gtactggctc agttatgcga gggatgaagt gaaccgtcat
     2581 tccgaaccct tcgtcggagt ctatcctgag caacgccgaa ggactcaggg caggctcagt
     2641 cttcgcgcgt agtgaggaat ctctcttaca gtccgaatcc tgtctgcaaa gg
//
LOCUS       DAOQUC010000453         2690 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_45212_length_2690_cov_3.116888, whole genome shotgun sequence.
ACCESSION   DAOQUC010000453 DAOQUC010000000
VERSION     DAOQUC010000453.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45212_length_2690_cov_3.116888"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..325
                     /locus_tag="PKH07_08675"
     CDS             <1..325
                     /locus_tag="PKH07_08675"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNT35057.1"
                     /translation="QRQMHERRFAEQQFQVIQIAGEDVGILATVREVDCIKVNQLFIL
                     PEYQGRGIGKACMAQVLASAKTEGLPVRLRVLKASPRAEAFYRALGFKETGETGTHIE
                     MERVP"
     gene            complement(924..1964)
                     /locus_tag="PKH07_08680"
     CDS             complement(924..1964)
                     /locus_tag="PKH07_08680"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNT35058.1"
                     /translation="MSILEALPKEEVIKAVERRNPIRVPVSMARWWGEGLHEQYGDRL
                     SAFDKYPDDCCRIWFPQPGTTPRGDGYYWRLDEEHLRKSRMGHDAGAVTGWEWLDAYE
                     SEIPNFEAPGLFDPFMPIVEEARKRNQYLILCWWGLFYEPIWGLRGMENLLMDYYLHP
                     EEIHRLHKARLAFYEGLIRRAAEEIAPDAFQSSDDLGHQTQLMMNPTQFREFIKPYYT
                     SLYRLSHKLGMHTWLHTCGNVTDIMCDLIESGLDVVHPIQKGAMDGEQIAKDWGDKIS
                     FWAGMDVQHALQEETPEGVRREVRWMIDTYDGHKGGLIIAAGNGIVAGTPFDNIEAFL
                     DESYRYGTEHRR"
     gene            complement(2190..>2690)
                     /locus_tag="PKH07_08685"
     CDS             complement(2190..>2690)
                     /locus_tag="PKH07_08685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35059.1"
                     /translation="RRYLDRGVRILRVIPAFHPSVLDEMNLKGLAEFLAGEEIVLLFQ
                     WMIEDYRSFHGLVRPAFPSRQQVLGFLSQMPCDVVLGGITYIEAEHILANTGGKVFVE
                     ISYIEKTRAMEDLVTPENSNRLLFGTMAPLLVPEAATMKVQMSRLDAKTKIRIFSQNA
                     LQLIGG"
BASE COUNT          637 a          695 c          722 g          636 t
ORIGIN      
        1 acagcgtcag atgcacgaaa gacgcttcgc tgaacaacag tttcaggtga tccagattgc
       61 tggagaggat gttggcattt tggcgacagt ccgtgaggtg gattgcatta aagtcaacca
      121 gctgttcatc ctgccggagt accaaggcag aggcataggg aaagcctgca tggcacaggt
      181 tttggcgtct gccaagacgg aagggcttcc cgtgcgcttg cgagttctca aagcatcccc
      241 gcgagcggag gccttctatc gtgccctggg atttaaggag actggtgaga ccggtaccca
      301 catcgagatg gagagagtgc catagcaggc gcgtgtggtc caacgtgcag aacgttcaac
      361 tcaaagagcc aatacattgc cgcgagaaga acgcatctcc atggacagcc tttgagaagg
      421 tcgagagatt ctacatcatt gaggctcttc acgtgcgacg gcctggatat ctacaccaaa
      481 ggaaagattc tgagaaagga aggatttgac gtattctatg tcgtcatctc gtcgaaaagg
      541 agcacagata tctgaagcgc taccgtgccc tgagtgtggg gcgatgcaga tggcctatac
      601 actcgaggat tgcaggactg aggatggtct aaccatccga cgccttgggc accttaagtt
      661 tagtgcttgt ggcactcgct tctttgatga cgatgccgtt caccgcatcc aaaaggagcg
      721 ggccagtcac ccgcttgtgc gtgctgtgtg actcacacgt tcgagcaaag cattggagtg
      781 gacgtactaa cgcgaagcgc tcagtttggg cgcgttggaa gcctttccct attcggatat
      841 agcagccctc agaagtgctc cgcattccta aaggcgaccc agaggagtga gcgggagcat
      901 gagagcaaag aattcacatg gcactacctt ctgtgttctg tcccgtagcg gtagctttca
      961 tccaggaatg cttcgatgtt gtcaaagggt gttccagcaa cgatgccgtt gcccgcagcg
     1021 atgatgaggc ctcctttgtg gccgtcatag gtgtcgatca tccagcggac ttcgcgacga
     1081 acgccttccg gagtctcctc ttgcagcgcg tgttgaacat ccattcctgc ccagaaggat
     1141 atcttgtcgc cccagtcttt ggcgatctgt tcaccgtcca tagcgccctt ttggatagga
     1201 tgcacaacgt cgaggcctga ttcgatcagg tcgcacatga tgtcggtgac attgccgcaa
     1261 gtgtgcaacc aggtgtgcat tccgagtttg tgggagagtc tgtagagcga agtgtaataa
     1321 ggcttgatga actctcggaa ctgagttggg ttcatcatga gctgtgtttg atgacccaga
     1381 tcatcactgc tctgaaaagc atctggggcg atctcctctg ctgctctcct gatgagacct
     1441 tcgtaaaagg ccagtcttgc cttgtggaga cggtggattt cctcaggatg gagatagtag
     1501 tccatgagca agttctccat tccccgaagt ccccatatcg gttcatagaa aagcccccac
     1561 cagcagagga ttaggtattg gtttcgcttt cgcgcttctt cgactatggg catgaaaggg
     1621 tcaaatagcc ctggcgcttc aaagttcgga atctccgatt cataagcatc aagccattcc
     1681 caacctgtga ccgcgccagc gtcatgaccc atgcggctct ttcgcaggtg ctcttcatcg
     1741 agtcgccaat agtaaccatc gcctctcgga gtggtgcctg gttgcggaaa ccagattctg
     1801 cagcagtcgt ccgggtattt gtcaaaagca gagagcctgt caccatactg ctcgtggagt
     1861 ccttctcccc accagcgcgc catggaaacg ggcacacgaa tggggttcct tctctcaaca
     1921 gccttgatta cttcctcttt cggaagtgct tccaatatgc tcatcttctc tggattctcc
     1981 tgtcagcagt caatttctgg aacaatagca gttgggaaat agagcaacaa gctgatgctc
     2041 tccatcagct caaagcgtta ctctgtgctc tgcggagagg gctctctccc gaagtcagac
     2101 ggagagtttc cgaaaagcct ccaagctgtg aagaaaggtt tacccaagtg gcctcaggat
     2161 aattcgcctg aatctgcatg gctgctgcac tacccaccga tcagctggag ggcgttttga
     2221 gagaagatgc ggatcttcgt cttggcatcc agccgagaca tttgtacctt catcgtggct
     2281 gcttcaggaa cgagaagcgg cgccatggtg ccgaagagaa gtctgtttga gttttcggga
     2341 gtgaccagat cttccatggc acgcgtcttc tcaatgtagg aaatctcaac aaagactttc
     2401 ccgcccgtgt ttgcaagaat atgctccgct tcgatgtagg taataccgcc aagcactaca
     2461 tcgcagggca tttgtgataa aaagcccaaa acctgctgcc ggctggggaa agcgggcctg
     2521 accaaaccgt ggaacgaacg atagtcctcg atcatccatt ggaaaagaag cacaatttcc
     2581 tcaccagcaa gaaactcggc cagacctttg agattcatct cgtcaagcac cgaagggtga
     2641 aaagcgggaa tgactcgaag aattctcacg cctcgatcca agtatcttcg
//
LOCUS       DAOQUC010000454         2689 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_45225_length_2689_cov_3.916097, whole genome shotgun sequence.
ACCESSION   DAOQUC010000454 DAOQUC010000000
VERSION     DAOQUC010000454.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2689
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45225_length_2689_cov_3.916097"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1126)
                     /locus_tag="PKH07_08690"
     CDS             complement(<1..1126)
                     /locus_tag="PKH07_08690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35060.1"
                     /translation="MATIPEYAKRAGVRVQRLLDFLEQSGHHGVSLDMELPDELSQLL
                     ESKFPLRSSDSSQMQGLREAALGGSEQEPGDTESAEDETQEPIPEIDETNAGVGKPLP
                     EQTPTPPEQQTEPVVEEQTPEELEITNLLKSLGSVEPEPEPEPEPEKEAAVQESEPET
                     EPVLPEPERPSPEEVPTEPEPETEVSETIDLEAFLQQLDSAQEPSSKTESEPSEPSEP
                     AQSASEPTEGEEETVELEELLKSLGQEVKSDATAAEPKVAKPAEAVPAAAPKTPTEKP
                     AGVKAQAPKPVHEEVVSERPSRRRPLPKPLELALNYSLQGVALTKDVWKEISLSLNFW
                     QKGILLGLVLATTGIVVGAIVKHSMDHAPGVEKSLLDEAQT"
     gene            complement(1130..2497)
                     /locus_tag="PKH07_08695"
     CDS             complement(1130..2497)
                     /locus_tag="PKH07_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNT35061.1"
                     /translation="MSVRTVLVADDEESIQKLLKRVLEREGYRVVVASDGAEAVEVAA
                     EERPSLGLFDLKMPGMDGVQALEAIRQQGLQFPVVMVTAHSTVESAVKAMKLGAHDYI
                     KKPFDVEELKLIIQKALEFSSLEREVLRLRRDVQCKYSLDQIVGEDPKMKEILQMIQT
                     VAATRSTALICGESGTGKELIARAIHYLSPRMDGAFVKVNCAALSENLLESELFGHEK
                     GAFTGAIRTHQGKFEQADGGTLLLDEISEMSASVQAKLLRVLQEKEIDRVGGRDPIQV
                     DVRVIATTNRDLEIECKEGHFREDLFYRLNVVKMAFPPLRERRGDIPLLAEHFRNLYA
                     EDMGKPVERISAEAMDALVRYDWPGNVRQLENALERAVVFVTGDTILPAHLPPEIGGV
                     APESQTATAEAWVGRTIEEIEREVILSTLKRTQDNRTQAADLLGISVRTLRNKLNEYR
                     EAGVI"
     gene            complement(2494..>2689)
                     /locus_tag="PKH07_08700"
     CDS             complement(2494..>2689)
                     /locus_tag="PKH07_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNT35062.1"
                     /translation="KEKGTGLGLSVSHRIVQEHGGLIQVVSEESKGTTFTVLLPLYAG
                     GYLAEVTNDLQTILRGIQTR"
BASE COUNT          547 a          804 c          667 g          671 t
ORIGIN      
        1 gcgtctgtgc ttcgtccagg agactctttt ccacaccggg tgcgtgatcc atgctgtgct
       61 tgacgatcgc tccaaccacg atgcccgttg tcgcgagcac caatccgagt aagatccctt
      121 tctgccagaa gttcagggac agggagattt ccttccaaac gtccttggta agtgcaacgc
      181 cctgcaggga gtaattcaag gccagttcca aaggcttggg caaaggtctt cttctgcttg
      241 gccgttcaga aacaacctct tcgtgcactg gtttgggagc ctgagccttg acaccggcag
      301 gtttctcggt cggagtcttc ggagcagctg caggaaccgc ttccgcgggt ttggcaacct
      361 ttggctcagc agccgtcgcg tccgatttca cttcctgccc aagtgacttg aggagttctt
      421 cgagttcgac cgtctcttct tcgccttcgg ttggctcgga ggcggactgt gctggttctg
      481 atggttctga tggttctgac tctgtcttgg acgatggctc ctgagctgaa tcgagttgtt
      541 gcaaaaaggc ttccaggtct atcgtttcac tgacttctgt ctccggttct ggttccgtag
      601 gtacctcttc cggtgaaggc cgttcgggtt ctggaagaac cggttcggtc tcaggctcag
      661 attcctgaac agcggcttct ttctcaggct ctggctctgg ctccggctct ggctcgaccg
      721 aaccaaggga cttcagaagg ttggtgattt caagctcttc gggggtctgt tcctcaacaa
      781 caggctcagt ctgttgttcg ggtggagttg gagtctgctc tggaagaggc tttccaacac
      841 ccgcgttggt ttcatcaatt tcaggaatgg gttcctgcgt ctcatcttct gccgattccg
      901 tatcgccagg ctcttgttcg gagcctccaa gggctgcttc gcgtagcccc tgcatttggg
      961 aagagtctga tgaccgaagc gggaatttgg actcaagcag ttgagaaagt tcgtcgggca
     1021 gttccatatc caacgaaaca ccgtgatgcc ctgattgctc caggaaatcc aacaggcgtt
     1081 ggactcggac acccgctctc ttggcatact caggaatcgt tgccacccgc tagatcactc
     1141 ctgcttcccg atattcattg agcttgtttc gcagtgttcg gacactgatg ccgagcaagt
     1201 ccgccgcttg cgtccgattg tcctgcgtcc tttttaaggt gctcaatata acttcccgtt
     1261 cgatctcctc aattgttcgt ccgacccagg cctcggcggt cgccgtctgg gattcgggag
     1321 caactccccc gatttcaggg ggaagatgtg ccggcagaat tgtgtcacct gtgacaaaga
     1381 cgacggcgcg ttccaatgca ttctccagct gccgaacatt cccaggccaa tcatagcgca
     1441 ccagcgcatc catggcctcg gcggagatac gctcaaccgg cttgcccatg tcctcagcat
     1501 agagatttct gaaatgttca gccagaagag gaatatcgcc gcgacgttcc cgaagtggag
     1561 gaaaagccat cttgacaacg ttcagacggt agaacaaatc ctcacgaaaa tgcccttcct
     1621 tgcattcgat ctccaagtct cgatttgtcg tggcgatgac tcgaacatcc acctgaatcg
     1681 gatcgcggcc tccgacacgg tctatttcct tttcctgcaa gacacgaagc aacttcgcct
     1741 gtaccgaggc actcatttcg ctgatctcat ccagcagcag cgttcccccg tccgcctgct
     1801 cgaatttccc ctgatgcgtt ctgatggcgc cagtgaaagc gcctttctcg tgaccgaaca
     1861 actcactctc gagaagattc tcggacaacg ccgcacagtt gaccttgacg aaggcaccat
     1921 ccatcctcgg actcaggtaa tgaatggcac gtgcgataag ttcttttcct gttcccgact
     1981 caccacagat cagcgccgtg gaacgtgtcg cggcgactgt ctgaatcatc tggagaatct
     2041 ccttcatctt gggatcttcc ccgacgatct gatccaaact gtacttgcac tgaacgtcgc
     2101 gacgcagacg cagaacctcg cgctcaaggc tcgagaactc aagtgccttc tggatgatca
     2161 gtttcagctc ctcgacatcg aagggcttct tgatatagtc atgggctccc agtttcatcg
     2221 ccttcacagc cgattccact gtcgaatgtg ccgtcaccat caccacaggg aattgaagac
     2281 cctgctggcg aatcgcctca agagcctgca ctccatccat accaggcatt ttcagatcaa
     2341 aaagccccag actcggccgt tcctctgcgg caacctcaac tgcctcagct ccgtccgaag
     2401 caacgaccac acgatagccc tcacgttcca agacccgttt cagcaacttc tggatgctct
     2461 cttcgtcatc cgctacaagc accgttcgaa cgctcatctt gtctgaatcc ctctcagaat
     2521 agtctggaga tcattggtga cctctgcaag ataaccaccc gcgtaaagag gcagcagcac
     2581 cgtaaaagtc gtgcctttcg actcttcact gactacctgg atcagtcctc catgctcctg
     2641 cacgattcgg tgggaaactg aaagcccgag accggttccc ttctccttg
//
LOCUS       DAOQUC010000455         2688 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_45258_length_2688_cov_3.464109, whole genome shotgun sequence.
ACCESSION   DAOQUC010000455 DAOQUC010000000
VERSION     DAOQUC010000455.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2688
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45258_length_2688_cov_3.464109"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(151..816)
                     /locus_tag="PKH07_08705"
     CDS             complement(151..816)
                     /locus_tag="PKH07_08705"
                     /inference="COORDINATES: protein motif:HMM:NF014696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HNT35063.1"
                     /translation="MATNKSNEFISLWERLADRIGLNRLFLRQGETRLDERDEILISP
                     IDGKVQHIAPIGTDGVIQEKSLFGKPRFVCLKDHLCDSPHMKAFLGGHYINLYLAPWN
                     LHYILFPAAGKVVETRYLPGACYPLVFCRMGDVKNEKLFAIVETRWGFPIAMVLVGSF
                     AVCGIHLDAKQGKDYEKGHRLGCFKLGSTVVMAFPPGKVETLVEERQRIRLGDPLARL
                     LCS"
     gene            1071..1691
                     /locus_tag="PKH07_08710"
     CDS             1071..1691
                     /locus_tag="PKH07_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020249238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNT35064.1"
                     /translation="MRRKGFTLIELLIVVAIIGILAAIAIPNFLAAQVRAKVARAKSE
                     MKTLQTAAETYLVDCNMYPRNTSEPPYSTMGDGGGPLWGSGYCSPDFTTPIAYIKKAA
                     LRDPFNDVPGLSSTPEDEIIYSYHTIGPNRAWTDTTGKFRNVYGKYRFTSYGPDRNYW
                     NNPTNTASRTQRIIYDPTNGTVSQGNIFISQKAGIMTKEPLESDLY"
     gene            1881..>2688
                     /locus_tag="PKH07_08715"
     CDS             1881..>2688
                     /locus_tag="PKH07_08715"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF009855.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HNT35065.1"
                     /translation="MNKNSLIAIDVGNTNIALGLFTPEGELTHTYRLVTDRWRTADEI
                     QVLWTPFLQHAGADPDFVLSSVVPPATRELVRAVHKYYSKGVYCVIPTELNAMPVKLH
                     RPEELGPDRLVNAVAGRDLYGPPLIIVDLGTATTYEIVSEQGEYIGGAICPGLHISAQ
                     ALFEKTALLQRVEVVPPPSAIGKSTIECLQVGLFYGLVAQVEGLVERMQKALPHRAKV
                     IATGGYAELLRDQTPSVDIIDPDLTLKGLQILYRLSQEGAIHLRCTNSSST"
BASE COUNT          641 a          711 c          703 g          633 t
ORIGIN      
        1 gtccaaagga gtgggcatag cccacgcgcc tgcaggtgct aatctgctcc tcgtgacact
       61 gagtccgaca gcggcctgtc atataccgat caatcgtcca agggagtggg catagcccac
      121 gcgcccgcag gcgctaacct gctcaggata tcaggaacaa agcagcctgg ccaaaggatc
      181 gcccaaccga attcgctgtc tttcctccac aagggtctcc actttgccgg gtggaaacgc
      241 catcacgact gtcgagccga gcttgaaaca cccgagccgg tggccctttt cgtagtcttt
      301 gccctgtttg gcatccagat ggattccaca gacggcaaag gagccgacca gcaccatcgc
      361 gattggaaat ccccatcttg tctccacgat tgcgaagagt ttctcattct taacatctcc
      421 catacggcaa aataccaacg gatagcacgc tccaggcaag tagcgagtct ctacaacctt
      481 cccggcagca gggaaaagaa tgtagtgcag gttccaggga gcgagataca gattgatgta
      541 atgtcccccc aggaacgctt tcatgtgagg ggaatcgcag agatggtctt tcaggcacac
      601 gaaacgaggt ttgccaaaga gtgatttctc ctggatgact ccgtccgtgc ctatcggagc
      661 gatgtgctgg actttgccgt caatagggct gatgagaatc tcatctcgtt catccaaccg
      721 ggtttccccc tggcggagga agaggcggtt caagcctatc cggtctgcca gccgttccca
      781 aagcgagatg aattcatttg acttgtttgt tgccatttga ttagaatgct tattcaacat
      841 gggcagtagg ttaaatgaag aaaggaggat gcgcaacacc gtcgtttttg gtattgctaa
      901 tgaagaccag atattctgag tctttagccg aaggcgagac agggattcat cctgattggg
      961 atatgcaggc tttatagaat cttgtcttga taaaaccatt taggatgcat ccgtaagtca
     1021 agccttcatg agaattgttc ggagaacaaa tacccaggag gtagatttcg atgcggagaa
     1081 aaggtttcac tttgattgag ttgctgatcg tcgtcgccat catcggcatt ttggcggcaa
     1141 tcgcgatccc gaactttctg gcagcgcaag tgcgagcgaa agtcgcccgt gccaagtcgg
     1201 aaatgaagac cctgcagaca gcggcggaaa cttatctggt agactgcaat atgtatcctc
     1261 gcaacaccag tgagccgccc tacagcacga tgggcgacgg tggtggtccc ctttggggtt
     1321 ccggctactg cagcccggat ttcacaacgc ccattgccta tatcaagaaa gccgcgttgc
     1381 gggatccctt caatgatgtt cccggccttt cgagcacacc cgaagatgag attatctatt
     1441 cgtatcatac gatagggcca aacagagcct ggacggatac gactgggaag ttcaggaatg
     1501 tatacgggaa atatcgcttc acttcatacg gtcccgaccg aaattactgg aacaatccaa
     1561 ccaatactgc ttctcgaaca cagcggatca tctacgatcc gaccaatggc actgtcagcc
     1621 agggcaacat cttcatctcc cagaaggctg ggatcatgac caaagagcct ctggagtcag
     1681 atctgtatta gtctggagtt ctgtctcgat gacttattca gagccacagg gaatttccct
     1741 gtggctcttt tgcttgggtg tggggctgac tgacttctcg catcattccg cttgcctatg
     1801 cgcctcaatt cgtatatgtt gtgcttatca acgttgtttc gcccatcata ttgggctgaa
     1861 tcgcagaaga atgggtgctc atgaacaaga attcactcat tgccattgat gtcggaaata
     1921 caaatatcgc tctgggtctt ttcacgccag aaggcgaact cacgcacaca tatcggctcg
     1981 tcactgatcg atggcggacg gcggacgaga ttcaggtgct gtggacgccg tttcttcagc
     2041 atgccggggc ggatcctgac ttcgtcctct caagcgttgt gcctccagcg acgcgggaat
     2101 tggtgcgtgc cgttcataag tactacagca aaggcgtcta ctgcgtcatt ccgaccgaac
     2161 tgaacgccat gcccgtcaaa ctgcatcgcc ccgaggaact tggccccgac cgccttgtca
     2221 atgcagttgc aggacgcgat ctgtacggtc ctcccttgat catcgtggat ctgggaacgg
     2281 caacaacata cgagatcgtt tcggaacagg gggagtacat cggaggcgcg atctgtcccg
     2341 ggctgcacat ttccgctcag gctctctttg agaagacagc tctcttgcag cgagtggaag
     2401 tcgtgccacc accttccgca atcggcaaga gcacgattga atgccttcag gtcggtcttt
     2461 tctatgggct tgtcgcccaa gtggaaggcc tggtcgaacg catgcagaaa gccctgccac
     2521 atcgggcaaa agtgatcgct accggaggct atgcggagtt gcttcgcgat cagactccca
     2581 gtgtagatat catagatccg gatctcaccc tgaagggatt gcagattctc tatcggctat
     2641 cacaggaagg ggcgattcat cttcgatgta cgaacagttc ttcaacct
//
LOCUS       DAOQUC010000456         2688 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_45269_length_2688_cov_2.327003, whole genome shotgun sequence.
ACCESSION   DAOQUC010000456 DAOQUC010000000
VERSION     DAOQUC010000456.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2688
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45269_length_2688_cov_2.327003"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(191..373)
                     /gene="tatA"
                     /locus_tag="PKH07_08720"
     CDS             complement(191..373)
                     /gene="tatA"
                     /locus_tag="PKH07_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013598249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HNT35066.1"
                     /translation="MGRIGPTELVIILCIVILIFGANRIPQLAKSLGEGIKEFRKSVK
                     TATKDEDDSAKPSDKA"
     gene            589..1980
                     /gene="cysS"
                     /locus_tag="PKH07_08725"
     CDS             589..1980
                     /gene="cysS"
                     /locus_tag="PKH07_08725"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014933939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HNT35067.1"
                     /translation="MLRFYSTMSRRKEDFIPLEFPKVGMYTCGPTVYNFAHIGNFRAY
                     VFEDILRRWLKHRGYQVTQVMNITDVEDKIIRDSTVQGVDIYTFTAKYTEAFFEDLDA
                     LRIERAEFYPKATDHIPEMIAIIKTLIEKGHTYERDGSLYFRLSTFSQYGKLSGVRPD
                     EVLSGARVDADEYEKDDARDFVLWKANKGESAWWDTELGRGRPGWHIECSAMSMKYLG
                     ESFDIHTGGEDNVFPHHENEIAQSEAATGRPFAKYWMHCRYLLVDGEKMSKSKGNFFT
                     LRDLLAKGHDPAAIRYLLASGHYRSPLNFTFDGLRAAESSVHRLKDCVLRLTEVASNA
                     AGTGHVFPMAQEAEKRFSDAMDDDLDMPNALAAVFDFVRAVNQAIDDRSLTPQDATAS
                     LEFLKTADSIMNVMDIGNDEILDQDIQRLIDERQAARKRRDFAGADAIRDQLAAMGIV
                     LEDTPQGVRWKRR"
     gene            complement(1999..>2688)
                     /locus_tag="PKH07_08730"
     CDS             complement(1999..>2688)
                     /locus_tag="PKH07_08730"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNT35068.1"
                     /translation="TLFLDEVSNISLEIQAKLLRVLESRKVKRVGDTAERDVRIRLIA
                     ATNQDLGELVQKRDFRADLYYRLNVVPIQVPPLRDREGDIPLLCMTFLERFRKATRTK
                     ARQFTPQAMNGLQDYSWPGNVRELRNIVERIAILCPSETVDVGDLPEEIRRVSLDGVV
                     RALPVTWEEFKRLKRRVEKTAVEDIERRFLQQALERTGGNVTKAAENIGMQRTNLHAL
                     IQKYGISVRQM"
BASE COUNT          619 a          707 c          712 g          650 t
ORIGIN      
        1 ttcagtgttt gactgagcaa gagtccaagt gggtgggaat agcccacgca cccacaggca
       61 cagacctgct tggaatgcac ggctttagcg ataacgagga atgctgcgaa taagagtgca
      121 cggtgtccaa aggcgccaag cctttgggca gggtggagag aacaggcgat tcggtcagaa
      181 aagtcggttt ctatgctttg tcgctcggtt tggcgctgtc gtcctcgtct ttggttgcag
      241 tcttcactga cttacggaat tccttaatcc cttcgcccaa agacttggcc agttgtggga
      301 ttctgttcgc tccaaaaatg aggatgacga tacagagaat gataacgagt tcggtcggtc
      361 caattcgtcc catggatgtt cacatccctt ctgatgtcag agcacgctca tcgccgagaa
      421 gaccgaaaat ctgagcgttt ctgcattgtc tacattaaga accagcgctc gggttgtcaa
      481 ctacattgtt gcagagcatc ttgttctctt ggggaaaaat ccttgccaaa cattctctcc
      541 tgaaggatat tgttcctggt tttcgatcga ttctcggagg tgtgatcagt gctgcgtttc
      601 tacagtacga tgagccgtcg caaagaggat ttcattcctc tcgagttccc caaggtcggc
      661 atgtatacct gcggaccaac tgtctacaac tttgcccaca tcggcaactt tcgcgcttat
      721 gtgttcgagg acatactcag gcggtggctc aaacacagag gctatcaggt tactcaggtg
      781 atgaatatca cggacgtcga ggacaagatc atccgcgact ccaccgtaca aggggtggac
      841 atctacacat tcaccgccaa gtacactgag gccttctttg aggatctgga tgcccttcgc
      901 atcgagcgag ccgagttcta tcccaaggca acggatcata ttcccgagat gatcgcgatc
      961 atcaagacgc tgatcgagaa aggtcatacc tacgagcgcg acggcagtct ctattttcga
     1021 ctctccacat tctcgcaata cggcaagttg tcaggagttc gtcctgacga ggtgctgtcg
     1081 ggtgctcggg tggatgccga cgaatacgag aaagacgacg ctcgtgactt cgttctttgg
     1141 aaggccaaca aaggggaatc cgcgtggtgg gacaccgagt tgggacgtgg gagaccaggt
     1201 tggcacatcg aatgttcggc catgtccatg aagtatctgg gcgaatcatt tgatattcac
     1261 acgggtggcg aagacaacgt tttcccgcac catgagaacg agatcgccca aagtgaagcg
     1321 gcgaccggca gaccgtttgc caagtactgg atgcattgtc ggtacctgct ggtggacggt
     1381 gaaaagatgt cgaaatccaa ggggaatttc ttcacgcttc gcgatctgct ggcgaaagga
     1441 cacgatcccg ctgccattcg atatcttctc gcttcgggac actacagaag tccgttgaac
     1501 tttacttttg acggtcttcg agcggccgaa tcctcggttc atcgcctgaa ggattgcgtg
     1561 ttgcgtctga ccgaggttgc ctccaacgcg gcaggtactg gacacgtctt cccgatggca
     1621 caagaagccg aaaagcgatt ctctgatgca atggacgacg atctggatat gcccaacgcg
     1681 ttggccgccg tttttgactt tgtccgagcg gtcaaccaag ccattgacga caggagcctg
     1741 actccgcagg acgcgacagc atccctcgag tttctcaaga ccgctgactc aatcatgaac
     1801 gtcatggata tcggcaacga tgagattctc gaccaggaca ttcagcgcct tattgacgag
     1861 agacaagctg cccgaaaaag acgggatttc gccggagccg atgccatccg agatcagttg
     1921 gctgcgatgg gaatcgtgct ggaagacact cctcaggggg tgcgttggaa aagacgctga
     1981 agacttttca ccgctcgtct acatctgtct gaccgaaatg ccgtatttct ggatgagcgc
     2041 gtgcagattc gttcgctgca tcccaatgtt ctctgctgct ttggtcacat ttccgcctgt
     2101 tcgttccaat gcctgttgga gaaagcgacg ctcaatgtcc tctaccgccg tcttctccac
     2161 ccggcgtttg agccgtttga attcctccca cgttaccgga agcgccctca ccacaccatc
     2221 gagactgact cttcgaatct cttcgggcag atcgccaacg tcaacagttt ccgaaggaca
     2281 gagaattgcg attctctcca cgatgtttcg aagctcccgc acgtttccag gccaggagta
     2341 gtcttgcagt ccgttcatcg cctgcggggt gaactgccgg gctttggttc gagttgcttt
     2401 cctgaatcgc tcaagaaaag tcatgcagag aagcggaatg tccccctcgc gatctcttaa
     2461 aggtggaacc tgaatcggaa cgacattgag ccgatagtac aagtcagctc ggaaatcacg
     2521 tttttgaacc agttcaccaa gatcctgatt ggttgcagcg atgagtcgga ttctgacgtc
     2581 cctttccgct gtatcgccaa ccctcttgac cttgcggctc tccagaacgc gcaggagttt
     2641 tgcctgaatc tccagactga tgttggagac ttcatcaagg aaaagagt
//
LOCUS       DAOQUC010000457         2684 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_45358_length_2684_cov_3.200837, whole genome shotgun sequence.
ACCESSION   DAOQUC010000457 DAOQUC010000000
VERSION     DAOQUC010000457.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2684)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2684)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2684
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45358_length_2684_cov_3.200837"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1263
                     /locus_tag="PKH07_08735"
     CDS             <1..1263
                     /locus_tag="PKH07_08735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35069.1"
                     /translation="GVLIAEDFNADGRRDLALYKGSTVPDGFHFIFNHGEVFFVTTSS
                     KDYSDFIDPYSAKYEWPSDATVAVAGNFDADGTPDLALLDFGTRVVSIRKAVSGGVTQ
                     PAYDYSVHLIKEGDLYRMWYGCRFIRLDEKGEKVRGGDGDHVCTAYSRDGRRWIRRSD
                     APALLKGEEEGYIGTWWSKNYLEPEVIKLGNLFYMYTQLMIDKEDLLDEPAVIAEGPA
                     DRMQLCLSPCGEKWTRFTERSVVINVDKPAKVKLTHHEVLYVPEDEQPFWFYVFWFEG
                     GTPKGHVRMRSSDLFTFDWQKREHVSGMAQIGNQLAYGDTVDGKRVFFRITHSASEQG
                     KRVPAMQVSGDGLKWSPPNALLAGCEEDLGCFFLGLSTIDGTGRLDRREDGSFHALYG
                     ATTAKTPVAPEIFWSEIGVGEVIIRIGE"
     gene            1275..2621
                     /locus_tag="PKH07_08740"
     CDS             1275..2621
                     /locus_tag="PKH07_08740"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNT35070.1"
                     /translation="MRIGRISQYQSLTMVVFAALILCSCTGKTSDVGEVVSIWHPWMM
                     DIGDDFLAALELFEKSNPQFDIQALYAANTLSSNQKLFLSIAGGVPPDVTFVDGPQVA
                     EWACRGALEPLDDYIAQAGIEPDDFWEPCWKQTVFDGQVYALTLVADPNFGFFWNKEV
                     FRDSGLDPESPPKTLEEMDQMAAQITRSESARLTRIGLIPWSVYGNANSIFTWGWAFG
                     GEFYDPETNKISADHPKVVEALEWMVSYAKQYDIRKISSLAAGFGSAEQNPFYTGRLG
                     MQPMVVYMLRDIDRYRPDLDYGITILPQPDGGEYNCSWVGGWCLAIPRGAQNPRGAFE
                     LIRWLCASSEGTTLVANAIGSTPGYRKSAAFEEARRDPRMRAFVEILENSRHQRPVMP
                     AQAFYMGELERAVDDALFGKKTPQQALADATARTQKELDGILERYYERKARRESTE"
BASE COUNT          599 a          643 c          790 g          652 t
ORIGIN      
        1 ggagtgttga tcgccgagga tttcaatgca gacgggcgcc gcgatctggc gctgtataaa
       61 ggctccaccg ttcctgatgg attccacttc atcttcaacc acggtgaggt tttctttgta
      121 acgaccagca gcaaggacta ttcggacttc attgatcctt actccgccaa atatgaatgg
      181 ccgtctgatg caaccgtggc tgttgcagga aactttgatg ctgacggaac gccggacttg
      241 gcgctcttgg acttcggaac gcgagtggtt tctatccgaa aggctgtttc aggtggggtg
      301 acgcagccag cgtatgatta ctccgttcat ttgatcaaag aaggcgatct ctatcgaatg
      361 tggtacggct gtcgttttat ccgtcttgac gaaaagggtg agaaggttcg tggtggtgat
      421 ggagaccatg tgtgtaccgc ttactccaga gacggtcggc gttggattcg tcgctccgac
      481 gcaccggcgc tgctgaaggg tgaggaagag ggatacatcg gcacctggtg gtcgaagaac
      541 taccttgagc cggaggttat caaacttggg aatctcttct atatgtatac tcagctgatg
      601 atagacaagg aagacctgct tgatgagccg gcagtaattg cagagggacc agccgatcga
      661 atgcagttgt gtctaagccc ttgcggtgag aaatggacgc ggtttaccga acgttcggtt
      721 gtcatcaatg tagacaagcc tgctaaggtg aaactgacgc accatgaagt gctttacgtc
      781 cccgaagacg agcagccctt ctggttctac gtcttctggt tcgagggagg tactcccaag
      841 gggcatgttc ggatgcgctc gtcggatctc ttcacctttg actggcagaa acgcgaacat
      901 gtctcaggga tggctcagat cggcaatcag ttggcatacg gcgatacggt ggatggaaag
      961 cgcgtattct tcagaatcac acacagcgct tcggagcaag gcaaacgcgt tccggcgatg
     1021 caggtttcgg gcgatggcct gaaatggtca ccccccaatg cgctgcttgc tggttgtgag
     1081 gaagatttgg gttgcttttt ccttggactt tcgactattg atggaacagg acgattggac
     1141 agaagagaag acgggagttt tcatgccctc tacggagcaa cgactgccaa gactcctgtc
     1201 gcgcctgaga tattctggag cgaaatcggt gttggagaag tcatcattcg catcggagaa
     1261 taggttgtgt tgatatgaga atcggtcgga tatctcaata tcagtctttg actatggtcg
     1321 ttttcgcggc actgattctc tgttcgtgca caggaaagac atccgacgtg ggcgaggttg
     1381 tcagcatctg gcatccttgg atgatggata ttggagatga ctttctggca gcgttggagt
     1441 tgtttgaaaa gagcaatccc cagttcgaca ttcaggcgct gtatgctgcc aacacgcttt
     1501 catcgaatca aaaacttttc ctttccatcg cgggtggtgt tcctcctgac gtcacgttcg
     1561 ttgatggtcc ccaagttgcg gaatgggcgt gccgcggcgc attggagcca ttggatgatt
     1621 acatcgctca agcgggcatt gagcctgacg acttctggga gccttgttgg aaacaaacgg
     1681 tctttgacgg acaggtctat gcgttgactc tggtggctga tccgaacttc ggtttcttct
     1741 ggaacaaaga ggtctttcgt gactcgggac tggatccgga gagtcctcca aagaccctcg
     1801 aagagatgga tcagatggca gcgcaaatca cccggtctga gagtgctcgc ctgacgcgga
     1861 tcggccttat cccctggtcg gtctacggca acgccaattc catcttcact tggggatggg
     1921 cattcggggg ggagttctac gatccggaaa cgaacaaaat ctctgccgac catccgaaag
     1981 ttgtcgaggc tttggagtgg atggtgtcgt acgccaagca gtatgacatc cgtaagattt
     2041 cctcgctggc tgccggattt ggctctgcgg agcagaatcc gttttacacc ggaaggcttg
     2101 ggatgcagcc gatggtcgtc tacatgcttc gagacattga ccgataccga ccggacttgg
     2161 actatggcat cactattctt ccacaacctg acgggggaga gtacaactgc tcctgggttg
     2221 gtgggtggtg tcttgccatc ccacgtggcg ctcaaaatcc acgcggagca tttgaactga
     2281 ttcggtggtt gtgcgcctct tcggaaggaa caactcttgt cgccaacgcg ataggttcca
     2341 cgccgggata tcggaagtcg gctgcgtttg aagaagcacg ccgggatccg cgaatgcgcg
     2401 cctttgtgga gattttggaa aactcaagac accaaaggcc agtcatgccg gcgcaggcat
     2461 tctacatggg agaactggag cgcgcggtgg atgatgctct cttcggcaag aagactcctc
     2521 aacaggcact tgccgatgcc acagcgcgga cgcaaaagga gttggacggc atcctcgaac
     2581 gctactacga acggaaagcg cggagagaga gtacggaatg aatcgaagaa acatactggt
     2641 cggtctcgct ttcatctcac cctgtttggt cggatttctg ggac
//
LOCUS       DAOQUC010000458         2683 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_45384_length_2683_cov_3.747717, whole genome shotgun sequence.
ACCESSION   DAOQUC010000458 DAOQUC010000000
VERSION     DAOQUC010000458.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2683
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45384_length_2683_cov_3.747717"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(246..2213)
                     /locus_tag="PKH07_08745"
     CDS             complement(246..2213)
                     /locus_tag="PKH07_08745"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HNT35071.1"
                     /translation="MLFAIKTLIIVLLLTTSVTVWAQEKEAEKKEEEPINWLGIFVMH
                     YHNRVGQFRQENPTLPPDRRNIVFVGDSITEGFALPGYFPGLPVLNRGIGADGIGFDN
                     DRGILHRMNESIFDCSPSAVFLLIGVNDLPQDPKTPEACIAGYRQIVEEILAKKPDTA
                     VILQTLLPVGLKYFWHEKVNPRISEFNDLIRALAQEKGLDVLDLHALYRDEKGFLPES
                     VSNDGLHLKPEGYRTWAEQAWALLLKRDLVTQQQYQARHSAVHYKDWQIRNGRFYDNG
                     KWVFLKIGKPLRNFADEVQVDKLIADLETIKKKGYNCLEINCYWHHFDTDGDGVPDVS
                     LEPLRRLMDTLYEKGIYPCLSVETYGVGGGQIPEGFWQKYPNAVAINDHGKPISDTEY
                     GTGVRVPSIFCGEYREAAHTFIKSLARALDTRKILYFETTVEPQYMGSENLCYSTHAR
                     KEFQAWATGRQIECPPMPEGFPIPASFRDLPVWNQFRSEFLSQWINDDAAAFREIAGE
                     KAFVAVDYLETDGPEMVNRLGNRHTFLSCLTSPNIIQVNWHWHLIRKTPNQVAYDTVR
                     QVMKETGRDWAIMEHMTFNGTDYSAQEAPAVLENTLKNGTRFGWEFVDLGASSANGFS
                     MYQDDWSPKPLIAVVDDAWAEWLTNIQKDEE"
     gene            complement(2335..>2683)
                     /locus_tag="PKH07_08750"
     CDS             complement(2335..>2683)
                     /locus_tag="PKH07_08750"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011361379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TdeIII family type II restriction endonuclease"
                     /protein_id="HNT35072.1"
                     /translation="VFLFDIKTAKPNISNFKDFKRTLLEWVAIYLAGNPDDDVHSYIA
                     IPYNPYDPKPYERWTMKGMLDLDHELKVAEELWDFLGRGGIYSEVLRCFERAGNVLRP
                     ELDKFFAAFAQAT"
BASE COUNT          630 a          745 c          657 g          651 t
ORIGIN      
        1 acgactgtct ccacaggaga gaaattcgaa agccgaagaa tcttgcgcac ggtgttctcg
       61 tccacaagga aattctgccc cttcaccttg tgaatcgcca aaccccgctc ttgaataatg
      121 tctcgaacct gtgccgctgt gagcatgatg accagtagcc tattgtgtta gagcgctcag
      181 gtcaaatgaa aggcgcggcg ctcgccggaa agtctggggt tggtctgaaa ctcaaaccgg
      241 ccaaatcatt cttcatcttt ttggatattc gtgagccact ccgcccacgc atcatccacc
      301 acagcgatca gcggcttggg cgaccagtcg tcctgataca ttgaaaagcc gtttgctgaa
      361 gatgctccca gatccacaaa ctcccagcca aatcgagttc cgtttttcag agtgttttcc
      421 agaaccgctg gtgcctcttg agcactgtaa tccgtcccgt tgaaagtcat gtgttccatg
      481 attgcccagt cgcgtccagt ttccttcatc acttggcgca ccgtgtcata ggcaacctgg
      541 ttcggagtct ttctgatcag gtgccagtgc cagttgacct ggatgatgtt cggactggtc
      601 agacaactca ggaatgtgtg ccgattgcca aggcggttga ccatctccgg cccgtcggtc
      661 tcaaggtagt caacagcaac gaaagccttc tcgcccgcaa tctctcgaaa cgccgcagca
      721 tcatcattga tccattgtga cagaaactcg gaacgaaact gattccatac cggaagatca
      781 cgaaacgacg cgggaatggg aaaaccttct ggcatgggcg gacattcgat ctgcctgcct
      841 gttgcccaag cctgaaattc ctttcgtgca tgggtgctgt agcagaggtt ttccgacccc
      901 atatactgag gctccaccgt ggtctcaaag tagaggatct tgcgagtatc caatgctcgc
      961 gcaagggatt tgataaacgt atgcgccgct tcccggtatt ccccgcagaa gatcgaggga
     1021 acccgcacgc cggtgccata ttcggtgtcg ctgatcggtt tgccatgatc gttgattgcg
     1081 acagcgtttg gatacttctg ccaaaaccct tccgggattt gtccaccacc aacaccatag
     1141 gtctccacag aaagacaagg gtaaatccct ttctcgtaga gggtatccat gagacgtctc
     1201 agcggctcca aagatacatc gggaactccg tcgccatcgg tgtcgaagtg atgccaatag
     1261 cagttaatct ccagacagtt gtagcccttc ttcttgatcg tctcgagatc ggcgatcaac
     1321 ttgtccactt gcacttcatc cgcgaaattc cgcaggggct ttccaatctt cagaaacacc
     1381 cacttgccat tgtcgtaaaa ccgaccgttc cgaatctgcc agtccttgta gtggacggca
     1441 ctgtgacgcg cttgatactg ttgctgggtc accagatctc gcttgagcag cagcgcccaa
     1501 gcctgctccg cccaggttcg gtacccttcc ggtttgagat gcaggccatc gttgctgaca
     1561 gactcaggaa gaaacccttt ctcgtcacgg taaagggcat ggagatcgag cacgtcaagc
     1621 cccttctcct gagccagtgc tcgaatcaga tcattgaact cagaaatgcg gggattgact
     1681 ttctcatgcc agaagtactt cagcccgacg ggaagaagcg tttgcaggat gaccgccgta
     1741 tcgggttttt tcgcgagaat ctcttcaacg atttggcgat atcccgcaat gcacgcttcc
     1801 ggagtcttgg gatcctgcgg cagatcgttg acgccgatca acagaaacac cgccgacgga
     1861 gaacaatcga agatactctc attcatccga tgcagaattc cccggtcatt atcgaatcca
     1921 attccgtccg cgccgatgcc acgattcaga acgggcagtc cgggaaagta acctggcagg
     1981 gcaaatcctt ccgtgatgga atccccgacg aagacgatgt tcctgcgatc ggggggcagc
     2041 gttggatttt cctgtcgaaa ctgcccgact cgattgtggt agtgcataac gaaaatgccc
     2101 agccagttga tcggctcttc ttcctttttc tcggcttcct tctcctgtgc ccagacggtc
     2161 accgaagtcg tcagaagcag aacaatgatt agcgtcttga tggcaaataa catgcccaaa
     2221 acctcccgtg tgcgtttact ggaaaccaat cccgcttccc gggagtggct ccttttgtag
     2281 ggtattcata ctacagtatt ctatttttgg acaaacgaag cgcgagaact ggccttacgt
     2341 tgcttgagca aacgcggcga agaacttatc tagctcgggt ctcaagacgt tgccagctct
     2401 ttcaaaacaa cggagcactt cagaatagat tcccccacgt ccaaggaagt cccacaattc
     2461 ttcggccacc ttgagctcat gatcgagatc caacatgccc ttcatcgtcc agcgctcata
     2521 gggtttcgga tcgtatgggt tgtagggaat cgctatgtat gaatgcacgt cgtcgtctgg
     2581 atttcctgcc aggtagattg caacccattc aagcaacgtc ctcttgaaat ccttaaagtt
     2641 actgatatta ggtttggcgg tcttgatatc gaaaaggaag acc
//
LOCUS       DAOQUC010000459         2680 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_45465_length_2680_cov_3.018667, whole genome shotgun sequence.
ACCESSION   DAOQUC010000459 DAOQUC010000000
VERSION     DAOQUC010000459.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2680
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45465_length_2680_cov_3.018667"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            209..898
                     /locus_tag="PKH07_08755"
     CDS             209..898
                     /locus_tag="PKH07_08755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35073.1"
                     /translation="MRNLRLACCLVGCLILSTGCSFLHFAKGQGPVTKSEAFALFNRT
                     PEKMPDDLHAQAQLLLRTAESKPVRTWKTKSGESISCRSAEPLSWTLLLGFHTKPSAF
                     LTERQGRMEEVVASRLLIAPLFPLFMPYMHGRVHFYDNGTGQEISFTEVDLLGFVGLL
                     GGTMRGAMTQSSGNRPPYTFVGEQDYRAFKGWYLLSGFLSYGTVNDTTYLQLAWIPIP
                     VHVQKRQTQSP"
     gene            1012..>2680
                     /locus_tag="PKH07_08760"
     CDS             1012..>2680
                     /locus_tag="PKH07_08760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35074.1"
                     /translation="MRPPLRILGRFAVLLLALSAAARFDPQGKDTTKMRFVCSESNDL
                     YSLLAQLGKVYPRHGTFAEAIAQASPKSAVLVLADQYPEKGTVIEETELDAARSKSLK
                     VYIEYPQKLPGFELGTPQKALWERGVVATDLFGFLLPKHRILDLHSCQFVPAQAPQHW
                     LSLGVVAGYDTAVYGIPEKAHPLLFQADKGALLIATTKLSNFVTGRYAPSKDWDIFWL
                     RLLMMLSPDDAPESLEHTPLVRARWGKNEALPENYQKQAFSAAAQWVYDSHLLLSKET
                     KAINDPLIINNVEWTSAPKPDEPIGDGRYGILEGYAAVIEPDGSQQRRTPIRADCHAE
                     SAMVLSLGWKMNQSQRDRDTARNLLNYVYFDSGMHDGVRGDPKNPNFGHIAWGALSHL
                     WEKANYGDDNARVLLGTMVASACLETDAWDQSMMRALLANLRTTGPLGFRGGRIDVVP
                     LETDGWKHYYDAERVHYSPHYESFMWACYLWAYRATGHREFFDKTKIAIRMTMEAYPD
                     GWLWQDSLERARMLLCLSWLVRLEDTPEHREWLHRVADDLLAYQVSCG"
BASE COUNT          592 a          717 c          737 g          634 t
ORIGIN      
        1 tactttgcgt tgacagtgtg agaagagtaa cgggaaaaac gaaaccttgt gctcctgttc
       61 gacagaggtc ttgacactga agagcgaatc gctattctcc ccttcgacca tgctgcgttc
      121 aatggtcaag tcaaagacca agggctgatg atttgatctt gcacctgtgc tgtgccgcta
      181 cactttgtgg acactgaaag gatttctcat gagaaatttg cgactcgcct gttgtcttgt
      241 tggttgtctg attctctcta cgggctgcag tttcctgcat tttgccaagg gacagggtcc
      301 agttaccaag agtgaggcat tcgccctgtt caacagaacg cctgagaaaa tgcccgacga
      361 tctccacgcg caggcgcagt tgcttctgcg aacggctgag tcaaagcccg ttcggacatg
      421 gaagaccaaa tccggggaga gtatcagttg tcgcagtgcg gaaccattga gttggacgct
      481 gctactcggc ttccacacaa agccctctgc gtttctgaca gagagacagg gccgaatgga
      541 agaggttgtt gcgtccaggc tcctgattgc acccttattt ccccttttca tgccctatat
      601 gcatgggaga gtccatttct atgacaacgg tacaggtcag gaaatctcat tcacggaggt
      661 agatctactc ggctttgttg gtctcctcgg tggaacgatg cgtggagcta tgacgcagtc
      721 atccggtaac agacctcctt acacatttgt cggcgagcag gactatcgag ccttcaaagg
      781 ctggtatttg ctgtcagggt tcttgtccta tggcacggta aatgacacga cctatctcca
      841 actcgcctgg attcccattc ccgtgcatgt tcagaaaaga cagacacagt caccctgaag
      901 tgcagcctct gcgtttgcac agtccgcctc ctctcaggac tcttctggtg cagtctgtgt
      961 ctcttgtgct ataagagcgc gaagacgatc ctgaaagttc gggatgttaa gatgagacct
     1021 ccattgcgca tcctgggacg ctttgctgtc ttgctcctcg ctctgagcgc agcagcgcgt
     1081 ttcgatcccc agggaaagga cacaacgaag atgcgatttg tctgctctga atccaacgat
     1141 ctctacagtc tgcttgccca gttggggaag gtctatcccc ggcatggaac gtttgccgaa
     1201 gccattgctc aggcaagtcc caagtctgcc gttctcgtgc tggccgatca gtaccctgag
     1261 aaaggcacgg tgattgagga gacggaactg gatgctgccc gatccaagtc gttgaaagtc
     1321 tacattgaat atccccaaaa actccctggc tttgagttgg gaacgccaca gaaagccttg
     1381 tgggagcgcg gtgttgtcgc gaccgatctc ttcggattcc tgcttcccaa gcaccgaata
     1441 ctcgatcttc acagttgcca gtttgttccg gcacaagcgc cacagcactg gttgtcgctg
     1501 ggtgttgtcg caggctatga cacggctgtc tacggcattc ccgagaaggc acatccactg
     1561 ctgtttcagg cagacaaggg agcgcttctc attgccacga ccaaactcag caacttcgtc
     1621 accggacgat atgctccgtc gaaggactgg gacatcttct ggctgcggtt gctgatgatg
     1681 ttgagtcctg atgacgctcc ggaaagcctg gagcacacac cgctggtcag ggcgcggtgg
     1741 ggaaagaatg aagccctgcc cgaaaattat cagaagcagg cattcagcgc cgccgctcaa
     1801 tgggtctatg attcgcatct ccttctcagc aaggagacca aagcgatcaa tgacccgctg
     1861 attatcaaca acgtcgaatg gacgtctgct ccaaagcccg atgaacccat tggcgacgga
     1921 cgctacggga ttctggaagg ctacgccgcc gtcatcgaac cggacggcag ccaacaaaga
     1981 cgaacgccga ttcgggcaga ctgtcatgcc gagtccgcca tggtgttgtc tctcggatgg
     2041 aagatgaacc agtcgcagcg ggatcgagac acggcgcgga atcttctgaa ctatgtctat
     2101 tttgattccg gaatgcatga tggcgttcgc ggggatccca agaatccaaa ttttggacac
     2161 atcgcctggg gagcactctc gcacctttgg gaaaaggcca actacgggga tgacaacgcc
     2221 cgtgttctct tgggaacaat ggtcgcctcg gcctgtctgg aaaccgatgc ctgggatcag
     2281 tccatgatga gagcactgtt ggcaaacctt cggacaaccg ggccactagg cttccgaggc
     2341 ggacgtattg atgtcgttcc tttggaaacg gatggttgga agcactatta tgacgccgag
     2401 cgtgtccact actcgccaca ctatgagtct ttcatgtggg cgtgctacct ctgggcgtat
     2461 cgtgcgacag gacatcggga gttctttgac aagaccaaga ttgccattcg gatgacgatg
     2521 gaagcctatc cggacggttg gctctggcag gacagtctcg aacgggctcg aatgctcctc
     2581 tgtctctctt ggctcgtgcg gttggaagat acaccggagc atcgcgagtg gcttcaccgt
     2641 gtcgccgatg atctccttgc ctatcaggtg tcgtgtggcg
//
LOCUS       DAOQUC010000460         2677 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_45552_length_2677_cov_3.028986, whole genome shotgun sequence.
ACCESSION   DAOQUC010000460 DAOQUC010000000
VERSION     DAOQUC010000460.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2677
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45552_length_2677_cov_3.028986"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..373
                     /gene="rph"
                     /locus_tag="PKH07_08765"
     CDS             <1..373
                     /gene="rph"
                     /locus_tag="PKH07_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015401725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HNT35075.1"
                     /translation="VLQADGGTRTASVTGASVALYDALSIFKQAGVITQWPLPYLVAG
                     VSVGIFKDQVVLDMDYREDSEAEIDMNVIMTEKGEFVEVQGTGESHSFSREQLDQMLD
                     LAGSGIRTLIGMQKKALGIEK"
     gene            complement(452..>2677)
                     /locus_tag="PKH07_08770"
     CDS             complement(452..>2677)
                     /locus_tag="PKH07_08770"
                     /inference="COORDINATES: protein motif:HMM:NF018606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35076.1"
                     /translation="IRQLVLAGAKWLDRDAWQQVHRWVKRGGVFLVLQDEPVSDIHGD
                     TSLWKAQQPADTGLVDIANRALTAETRQAIVDAVWSHSAKRIGKGWVLTLDTKDIPAD
                     RIAWIVSSLCMQAGTRVGKPDWNLKFLDNGIDGILSTLFPSRILYYNKTSQRKTLFLS
                     LREEDFPEGQPRPSSSQIRLEIDPKTIAVVPLDEANKFERPERATTRTGGQDRMKRAD
                     EILAKVAEYADNAIQYGRDVYGEKPSPLFVDGLNVLTRKPVIWRLPADSTKVSSKGMP
                     RESVMCNFASQQTLLRVLDGLTDLTGEPRYRQAATETIEYAFENLQHRTGLLFWGGHL
                     FVDLATRKFVGEAHRDASVGISGLAHELKCSYPHYELMYQVNPEATVRLICSIWANHI
                     VNWETLEFNRHGYYDVFPDGVWDHEYKGGEVPIVTKNMAFINTGSDLFYAGSMLYHLT
                     GEKPALEWSKRLLGRYEDIRHPKTGLAGYQFTIVPDRRVQRLFEAKHGTRINESTIVN
                     VGYTVQRHALIVVCRLRIAEMLGNAGKEFFDSAIADVVALARHGFDRETNTFWAMNND
                     GLILKPEEMQQEGYFTRADLGRRPVTGLLLWAYALAFRLTRDPFLQETVTSMGQGLGL
                     WDSAGTMDLETETSDVHTLMAALELYEATKEDACLSLAERIGTNLLEHQFRNGFFVNG
                     PRHINAPFGHQTPLALLSLISVMKEMPMPKGLPSNYFFHCEYEGLGRTYDKQAIYTQE
                     E"
BASE COUNT          601 a          727 c          729 g          620 t
ORIGIN      
        1 cgtgctgcag gccgatggag gtacgcgaac ggcgtcggta acgggagcct cagttgcctt
       61 gtatgacgcc ctcagcattt tcaagcaggc aggggtaatc acccagtggc cattgccgta
      121 tctggttgcc ggtgtcagcg taggcatctt caaggatcag gttgttctcg atatggatta
      181 tcgagaagac tccgaggccg agatcgacat gaatgtcatc atgaccgaga aaggcgagtt
      241 cgtcgaggtt cagggaaccg gggagtccca ttcattctcg cgagagcaat tggatcagat
      301 gctcgatctg gccggttccg gcattcggac gctgattgga atgcagaaga aagcgctcgg
      361 aatcgagaag tagggcaggc tccggttcgt gtttgcagcg acacactccg gcccacgcct
      421 cacaaaccca tacgcagcag acaatgacga tctactcttc ttgtgtgtag atggcttgct
      481 tgtcgtaggt tcttcccagt ccttcgtact cgcaatggaa gaaataattg ctgggcagtc
      541 ctttgggcat cggcatttct ttcatcaccg atatcaacga cagcaaagcc aagggagtct
      601 gatgtccgaa cggcgcattg atgtgacggg gaccgttgac aaagaaaccg tttcggaact
      661 gatgctcaag aaggttcgtt ccgattcgct cagcaagact gagacaggca tcctcctttg
      721 tcgcttcata cagttccaag gcagccatga gggtatgcac atcgctcgtt tcggtttcca
      781 gatccatagt gccggcagag tcccagagac caagcccctg tcccatgctg gtgaccgttt
      841 cctggagaaa aggatcgcgg gtcagacgga acgccagggc gtaagcccag agcagcaagc
      901 cggtcaccgg acgtctgcca agatccgcac gtgtgaaata accttcttgc tgcatctctt
      961 cgggttttag aatgagtccg tcgttgttca tcgcccagaa ggtgttggtc tctcgatcaa
     1021 agccatgtcg cgccaatgca acgacgtctg caatggcgga atcaaaaaac tccttgcctg
     1081 cgttgcccag catctcggca atccggagtc ggcatacgac aatcaaagca tgtctttgta
     1141 cggtgtaccc gacgttgaca atggtggatt cgttgatgcg tgtcccatgt tttgcctcga
     1201 aaagtctctg gactcgacga tcgggaacaa tcgtgaactg atagcctgcc aagccagtct
     1261 tggggtggcg gatatcttcg tagcggccca gcagcctttt cgaccactca agggcaggct
     1321 tctcacctgt gagatgatag agcatcgagc cggcgtagaa aagatcggaa ccggtgttga
     1381 tgaacgccat gttcttggtg acgatgggta cctcaccccc cttgtattca tgatcccaaa
     1441 ctccatcagg gaagacatcg tagtaaccgt gtcgattgaa ttcgagtgtt tcccagttga
     1501 cgatgtgatt cgcccatatc gaacaaatga gtcgaacggt ggcttccgga ttgacctggt
     1561 acatgagttc atagtgcgga tagctgcact tgagttcatg cgccagacca gagatgccca
     1621 ccgaggcgtc tcgatgcgct tcaccgacaa acttccgcgt cgccaaatca acgaacaggt
     1681 gtcctcccca gaagagcagg ccggtgcgat gctgaagatt ttcgaaggcg tattcgatcg
     1741 tttcggtcgc cgcctggcgg tatcggggct cacccgtcag atcggtcaac ccgtccagaa
     1801 cgcgaagcaa cgtctgctgg ctggcgaagt tacacatgac agattcccgt ggcatccctt
     1861 ttgaactgac cttggtgctg tcggcaggga gacgccagat gaccggcttc ctggtcagga
     1921 cattaagccc gtccacaaag agcggagatg gcttctcccc ataaacatcc cttccgtatt
     1981 gaatggcgtt gtctgcgtac tctgctacct tggcgagaat ctcatcggcg cgtttcattc
     2041 tatcctgacc tcctgttctt gttgtggctc gttccggacg ctcaaacttg tttgcctcat
     2101 caagaggcac cacggcaatg gtcttcggat caatctcaag tcgaatctga ctggaagacg
     2161 gtctcggctg accctcagga aaatcctctt cacgcagaga gagaaacagg gtctttcgtt
     2221 ggctggtctt gttgtaatag agaattctgc tcggaaacag ggtactcaga atcccgtcaa
     2281 taccgttgtc caggaacttg agattccagt ctggtttccc aacgcgggtg cctgcttgca
     2341 tgcacagaga actgacgatc catgcgatgc gatcagcggg gatgtctttc gtgtccagcg
     2401 tcagcaccca gcctttgcca atccgtttgg cggagtgcga ccaaacagcg tcaacgatgg
     2461 cttggcgtgt ctcagcggtc agagcccgat tcgcaatgtc caccaggccg gtgtcggcag
     2521 gttgctgtgc cttccataga gaagtgtctc catggatgtc cgaaacaggc tcatcctgaa
     2581 gaaccaggaa taccccaccc cgtttgaccc aacgatgcac ctgttgccag gcatcgcgat
     2641 caagccactt cgcaccggcc agcaccagtt gtcgaat
//
LOCUS       DAOQUC010000461         2677 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_45553_length_2677_cov_2.963768, whole genome shotgun sequence.
ACCESSION   DAOQUC010000461 DAOQUC010000000
VERSION     DAOQUC010000461.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2677
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45553_length_2677_cov_2.963768"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(91..834)
                     /locus_tag="PKH07_08775"
     CDS             complement(91..834)
                     /locus_tag="PKH07_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020263042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNT35077.1"
                     /translation="MIRVTNLAKRLGDKDILRGVNLDIHKGETMVILGRSGEGKSVLL
                     KHFVRLMKPDSGQVFIEGQEITHLRPSELVPVRVKVGMLFQGAALFDSMTIEENVGFA
                     LYEHSGLSKQEIRSRVIQELAHVQLTSQVMNKKPAELSGGMKKRVGLARAIIIRPNIM
                     LYDEPTTGLDPITADAIDELIIQMQRDLGITSVVVTHDMKSAFKVADRMALLNKGRIH
                     KVGSKEEFQNTDDPMVRQFILGQARGPMT"
     gene            complement(899..1669)
                     /locus_tag="PKH07_08780"
     CDS             complement(899..1669)
                     /locus_tag="PKH07_08780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNT35078.1"
                     /translation="MRRLRSILIFIGSVVNSVLQELGSFALMVLDTFRKTPARPWRWS
                     LLLEQMESIGFNSLPIVLLTSTFTGMIMVLQTGIQLERFGAKVYAVGGATVALARELG
                     PVLTSLVLAGRVGAGIAAELGTMKVSEQVDALETLATDPIHYLVVPRFVAACIMLPIL
                     ATLSNVVGILGGMVVAKLSLGLSYRLSYRTALEWLTFEDLYSGLAKTLFFGMIISLVG
                     CYHGMKTSGGAEGVGRSTTKAVVTGSVLILVSDYFLTE"
     gene            complement(1683..>2677)
                     /locus_tag="PKH07_08785"
     CDS             complement(1683..>2677)
                     /locus_tag="PKH07_08785"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF015736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HNT35079.1"
                     /translation="DLLEQMQQQLLVLSQNTRTSGFRHVSEMLDTVMQRIRVARDDHS
                     RVTGVPSGFDGLDDLTAGFQKSDLVVLAARPSVGKTSLALNVASHAAVENGIPVGVFS
                     LEMNAEEIIERLLCSAARVNLKSVKDGWIDDEEFDRLVSCGGVLSTSPIHIDDSANLP
                     ITDIFTRAIRLKAKEPKLGLIVLDYLQLITAPPTSGYRNENRQQEVAGICRSLKSLAR
                     ELDVPLLVLSQLSRASEKRHDDPTPRLSDLRDSGAIEQDADVVMFLYQAQEPGQQTSH
                     YSAGSGEEETPEENSMVTLTIAKQRNGPRGSVSLYFCKQYTRFENLAMEEAPPY"
BASE COUNT          641 a          772 c          663 g          601 t
ORIGIN      
        1 atgatcgccg cgacaacgac aagcccaccc acacgcaact cagttctttt taactgcatt
       61 ccaaactccc tttcccatgg ccacctgctc tcaggtcatt ggaccacggg cctgcccgag
      121 aataaactgc cgaaccatcg ggtcatcggt gttttgaaac tcttccttgc tacccacttt
      181 gtggattctt cccttgttaa gaagcgccat cctgtctgca actttgaacg cgcttttcat
      241 gtcatgggta acgaccacag aggtaatccc gaggtcacgc tgcatctgaa tgatcagttc
      301 atcaatggcg tcggcagtga ttgggtcaag acccgtcgtc ggctcatcat agagcataat
      361 gttcgggcgg atgataatcg cgcgagccaa gccgacacgc ttcttcattc ccccggacag
      421 ttccgccggc ttcttgttca tcacctggga cgtcaactgc acatgcgcca attcctggat
      481 cactcgcgaa cggatctcct gttttgaaag accagaatgc tcatagagcg caaatccaac
      541 attctcctct atcgtcatgg agtcaaaaag cgcggctccc tgaaacagca ttcccacctt
      601 gacacgcact ggcaccagtt cggaaggccg caggtgagtg atctcttgcc cttcaatgaa
      661 gacctgtccg gaatccggtt tcatcagccg aacgaagtgc ttgagcaaaa cactcttgcc
      721 ctcgccgctt cggccaagga tgaccatcgt ctcccccttg tgaatgtcca agttcacacc
      781 acgcaggata tccttgtcac ccaacctttt ggccagattt gtcactcgga tcatcggttg
      841 tccattggtg tttggatcca tattcctcat cccaggaacg tcacaagaag gtcagaagtc
      901 actcggtcag aaagtagtcc gagaccaaaa tgagcacgga accggtgacc acagctttgg
      961 ttgttgagcg accaactcct tcggcgccgc cgctggtctt catcccgtga tagcatccga
     1021 ccaaagaaat gatcatgccg aaaaaaagcg tttttgccag cccactgtac aagtcttcaa
     1081 aggtcagcca ttcgagcgct gttcgatacg aaagtcggta tgacagtccc agagagagtt
     1141 tcgccaccac cataccgccc agtatgccga ccacattgga cagtgtcgcc agaatcggca
     1201 gcatgatgca ggctgcaaca aatcgcggaa caaccagata gtgaattggg tcggtcgcca
     1261 acgtttccag agcatcaacc tgttcactga ctttcatcgt gcccagttct gcggcaatac
     1321 cggcacccac ccgacccgcc agcacaaggg aagtcagaac aggccccagt tcgcgtgcca
     1381 gagcaactgt cgctccccca acggcataga ccttggctcc aaatcgctcc aactggatgc
     1441 cagtctgcaa taccatgatc atgccggtaa atgtcgaggt cagcaacacg attggaaggg
     1501 agttgaagcc gatgctctcc atttgctcaa ggaggagtga ccatcgccat ggtctcgccg
     1561 gagttttccg aaaggtgtca agcaccatca gcgcaaagga cccaagctct tgcagaacgc
     1621 tgtttacgac ggaaccgatg aagatgagga tgcttctgag tcttctcatg gtctgcgaaa
     1681 tctcaataag gtggtgcttc ctccatggcc aggttttcga aacgtgtata ttgcttgcag
     1741 aagtagaggc tcacactgcc tcggggaccg ttgcgctgct tggcaatggt gagtgtgacc
     1801 atactattct cttcgggagt ctcttcctcg ccggaacccg cagaataatg agaggtctgc
     1861 tggccaggtt cctgggcttg atagagaaac atcaccacgt cggcatcctg ttcgatggct
     1921 cccgagtctc tgagatccga gagacgggga gtgggatcgt cgtgcctctt ctcactcgct
     1981 cgagacagct gagacaagac cagcagaggc acatccaact ccctggcgag tgacttcaga
     2041 gatcggcaga tgccagccac ttcctgctgt ctgttttcat ttcgatatcc ggaggtgggc
     2101 ggagccgtaa tcaactgaag atagtccagg acgatgagtc caagtttggg ttccttggcc
     2161 ttcaggcgaa tggctcgcgt gaatatgtca gtaatgggaa ggttggcgga gtcgtcaatg
     2221 tggatcggac tggtggagag aacgccacca caggaaacca gtcggtcaaa ttcctcatca
     2281 tctatccaac catccttgac gctcttgagg ttcaccctcg cggcactgca cagaaggcgt
     2341 tcaataatct cctcagcatt catctcaagg ctgaaaactc cgactggaat tccattctca
     2401 accgctgcat gggatgccac attcagtgca agtgaggtct tgccgacact cggacgagca
     2461 gccagaacca caaggtctga cttctgaaaa cccgccgtca gatcgtccag accgtcaaac
     2521 ccgctgggaa caccggtaac tctggagtga tcgtcacgag caacgcgaat acgctgcatg
     2581 acggtgtcca gcatctccga cacatggcgg aatccacttg tcctcgtgtt ctgagacagg
     2641 acaagtaact gctgttgcat ctgctccagg agatctg
//
LOCUS       DAOQUC010000462         2672 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_45700_length_2672_cov_3.167367, whole genome shotgun sequence.
ACCESSION   DAOQUC010000462 DAOQUC010000000
VERSION     DAOQUC010000462.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2672
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45700_length_2672_cov_3.167367"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..915
                     /locus_tag="PKH07_08790"
     CDS             <1..915
                     /locus_tag="PKH07_08790"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HNT35080.1"
                     /translation="EEVQTHPILEQALLEGKTLLLPRVVAGCGDMTFWRVRDLEYDLR
                     CEPPFGIPQPIPERCEPVSLAEASLVLVPGVAFDRCCNRLGRGKGYYDHALSQMRPKQ
                     VTVGLAFEDHVFREIPTTPNDIPVGLVVTEKYIYAHRSFTVTCDSPGATEHLGFCLGS
                     SLPSDKIVSLIAPLGSGKTVIVKGMVCARGGTVPATSPTYTLVNVYETQHQPFRHLDL
                     FRWNSGKATEENIEELLDILSEPGLIAVEWADRAEEHLPMQSPCIRLVAGRERHRVWQ
                     IETFLLEDSHWLDECLCLISHKGLVSGI"
     gene            972..1472
                     /locus_tag="PKH07_08795"
     CDS             972..1472
                     /locus_tag="PKH07_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013237572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HNT35081.1"
                     /translation="MLKGKAFKFGDDVDTDAIIPARYLTTTDPKELASHCMEDSTDPD
                     FIKRVQPGDIMVAGRNFGCGSSREHAPRAIKAAGVSCVIAETFARIFFRNSINIGLPI
                     LECAEAARAIKAGDTVSISLQEGVILDETTGKQFAAEPFPEFMQKIVDKGGLMAVAQE
                     MIKDGR"
     gene            1673..>2672
                     /locus_tag="PKH07_08800"
     CDS             1673..>2672
                     /locus_tag="PKH07_08800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35082.1"
                     /translation="MNRVLLLLCITVLVGANTIPSFADVGTNKREDEDQMTNIEMLTP
                     LMGGCGGVYFLAEPGELTVVVEKRDRNRGTFQTEFRALLLAPDRSVVDEAIIGDDGMP
                     ADSGIGPAQRVRLSAHVSRAGVYALNITMPQDRYGLESVWGFWTDCDKYLIETARGHR
                     DLRHQEPIVLESPGRSANICFQPRQKAFTMELSALPEGSSPLALYDGADNLIQSIPVN
                     EDGTVKTVIEADLKRDNTPWRLYLPSAKGIVIIDGLNQWNSDDRYPDICCWTAQRQSW
                     FSLLEYRWLLSPYMRVIYGQPREQGSLTFQIHNNSDREELVRLNVEFSDKPFSVELD"
BASE COUNT          596 a          652 c          761 g          663 t
ORIGIN      
        1 gaggaagtcc aaacccatcc cattctggaa caagccctcc tggagggaaa aacactgctt
       61 ctgcctagag ttgttgcggg ctgtggtgac atgacttttt ggcgtgtccg ggatctggag
      121 tatgacctcc ggtgcgaacc tccgtttggc attccgcaac ccattccgga acgttgtgaa
      181 ccggtctcac ttgctgaggc gtcactcgta ctggttcctg gagttgcgtt cgatcggtgc
      241 tgcaatcgtt tgggaagagg caaggggtat tacgatcacg ccttgagtca gatgagaccg
      301 aagcaggtga cggttgggct ggcatttgaa gatcatgtat tcagggagat tcccacaact
      361 ccgaatgata ttcccgttgg tctcgttgtc actgagaaat acatctacgc gcaccgcagc
      421 ttcactgtga cctgtgacag cccgggagcc acagagcatt tggggttctg tcttgggtcg
      481 agtcttcctt ctgacaagat agtcagtttg atcgcccccc tgggcagtgg caagacggtc
      541 atcgtgaaag gcatggtttg cgcgagaggt ggaacggttc cggcaaccag tccgacctac
      601 accttggtca atgtctatga aacacagcac cagccgtttc gccacttgga tctgtttcgt
      661 tggaatagcg ggaaagccac agaagagaat atcgaagaac ttttggatat cctctcggaa
      721 cctgggttga tcgctgttga atgggcggat cgggctgagg aacatcttcc gatgcagtct
      781 ccctgcatcc gtcttgtcgc gggtcgggaa agacatcgcg tgtggcagat tgaaaccttc
      841 ctgcttgagg attctcactg gctggatgaa tgcttgtgtt tgatttccca caagggattg
      901 gtttctggta tatgattgca ggtaggacta atcggaaata ggtgagaacc tttactgata
      961 cggaggagaa catgttgaaa ggaaaggcat tcaagtttgg agatgacgtg gacacagacg
     1021 ccatcatccc agcccgatat ttgacgacca cggatcccaa ggaactggca tcacactgca
     1081 tggaagattc cacagatcct gatttcatca aaagagtgca gcctggggat atcatggttg
     1141 ccggcaggaa tttcggttgt ggctcttctc gggaacatgc tccgcgcgcg atcaaggccg
     1201 cgggtgtttc ctgtgtcatt gcagagacct tcgccaggat atttttccgc aactcaatca
     1261 acatcggctt gccgattctt gaatgtgcgg aagcggccag agcgatcaag gccggtgaca
     1321 ccgtcagtat cagccttcag gaaggtgtca tccttgatga gacgacggga aaacaattcg
     1381 ccgcagagcc gttccctgag ttcatgcaga agattgtgga taaaggcggt ctgatggctg
     1441 tggcgcagga gatgatcaag gacggccgct agaacacgat tcctgcgctg attcacctga
     1501 gagtgactgg cgactcggtt ctttgtctgt cgttttcccg gtcagtctgc ccagagtcaa
     1561 taggccggac acaggcgaga ctcaagttca tccggactac tgcaaaactt ctggggtgtc
     1621 cgtctggatt tgaagtcacc cattccagca ggcagtttct tgggagatct ttatgaatag
     1681 ggtattgctt cttctctgca tcaccgtgtt ggttggagcg aacacaatcc cctcgtttgc
     1741 agatgttggc acgaataaga gagaagacga ggatcagatg acaaacatcg aaatgcttac
     1801 ccctctgatg ggaggctgtg gtggagttta cttccttgct gaacccggcg aactgactgt
     1861 cgtggtcgaa aaacgcgata ggaaccgtgg gacgtttcag acggagtttc gcgccctgtt
     1921 gctggctcca gatcgctccg ttgttgacga agccatcatt ggcgatgacg gtatgcctgc
     1981 cgacagcggg atcggtccgg cgcagcgtgt tcggctttcc gcccatgttt ctcgcgcagg
     2041 tgtctatgcg ctgaacatta ccatgccgca ggatcgctac ggcctggagt cagtctgggg
     2101 gttctggaca gactgcgaca agtatctcat cgaaacagcg cgtggtcatc gagacttgcg
     2161 ccaccaggaa ccgattgttc tggagagtcc tgggcggtct gcgaacatct gctttcaacc
     2221 acgtcagaag gctttcacga tggaattgtc ggcgctgcct gaaggttcat cacctctggc
     2281 attgtatgat ggtgctgaca acctgattca gtccataccc gtgaacgagg atggaaccgt
     2341 gaagacggtc atcgaggcgg atttgaaacg ggacaacact ccatggcggc tttatcttcc
     2401 ttctgccaag ggtatcgtga tcattgacgg tctgaaccaa tggaacagtg atgatcggta
     2461 tccagacatc tgttgttgga ctgcgcagcg tcagtcctgg ttctctcttc tggagtatcg
     2521 ttggcttctg tcgccctata tgagggttat ctatggacaa ccgagagagc aaggctcgtt
     2581 gacttttcag attcacaaca actccgaccg agaggaactg gtgcgtctca acgtcgagtt
     2641 ttcggacaaa cctttttccg tggaactgga ta
//
LOCUS       DAOQUC010000463         2666 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_45869_length_2666_cov_2.278820, whole genome shotgun sequence.
ACCESSION   DAOQUC010000463 DAOQUC010000000
VERSION     DAOQUC010000463.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2666
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45869_length_2666_cov_2.278820"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="PKH07_08805"
     CDS             complement(<1..186)
                     /locus_tag="PKH07_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNT35083.1"
                     /translation="MYHRQNAFTLIELLIVVAIIGILAAIAIPNFLAAQIRAKVAKSK
                     AEMQSLSLGLESYCVDHA"
     gene            395..1285
                     /locus_tag="PKH07_08810"
     CDS             395..1285
                     /locus_tag="PKH07_08810"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HNT35084.1"
                     /translation="MKISENRDSVFRRFDWGGLCGAKFAAILVIVLAGCATGGLTRLA
                     GDLFVPPEQEIKLGQQFAAEIPKEYKICTDEVIQAYVRDVGTQIATAASKDRSDVTYT
                     FTVIDDPEQVNAFAVPGGYIYVFTGLMLFAENEAELASVLGHEAGHIVGRHSANQLGA
                     RIGLSLVSSVALGQNPTLLQQIVASMATTGSVLKFSRDDEREADSLGLKYTIQGGYDP
                     NAMVSFMQRLHKKSGAGDSEVAAFLSTHPLTSERIGNLQVEMAKYGKTKGTLNEKAFK
                     RIQEQIKALPKDGSATSTAK"
     gene            complement(1271..2335)
                     /locus_tag="PKH07_08815"
     CDS             complement(1271..2335)
                     /locus_tag="PKH07_08815"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNT35085.1"
                     /translation="MAVFHAQKTEATTNEAERLREVFRPVAAVLAKAQRKYESMLRTG
                     VSEFLLEDFAIRNISSQSDFLDRMLETISSAQGKWLRAGITLLCTKVTGVDPITSVDV
                     AVACEMIHTASLMHDDVLDGASLRRGQPSASSRWGNSLAVLGGDLLLALAFNLLTRSN
                     SQACLEDISGSAVRVCLGEMAQQNASGSASVTEEEYLGIIDRKTASLFRSCARCGGLL
                     GKAERPIVDALGDFGHSFGMAFQIVDDLLDFLSEDSFSGKTLGTDISQGKTTLATIHH
                     LATKSPERRDNTVIAELEAEDLRKTGSLDFAFQKARYYSEASQEALGKIQTADSASLE
                     SLRSLSSFILERTPLQLLGR"
BASE COUNT          674 a          661 c          722 g          609 t
ORIGIN      
        1 cgcgtggtca acacagtagg actcaagccc gagagaaaga ctctgcattt cagccttgga
       61 tttcgcaacc ttcgcgcgta tttgcgctgc cagaaaattg gggatcgcta tggccgcaag
      121 aattccgatg atggcgacaa caatgagcag ttcgatcagg gtaaaggcgt tctgacgatg
      181 atacatggca agttacctcc tgtttcttct atcctagcgc aatgtgccaa cgagggggaa
      241 gctgttttct tgaacaacca gaagaatgtg atatgctcat aatagacagg agactgttga
      301 gtgacccaaa gcatgtaaca aggtggccag tcagcaaaca agagcaggcc ggatgtccga
      361 caagtgctgg ttgattccaa aggagtccga gattatgaag atatctgaga atcgagattc
      421 tgttttccgc agatttgact ggggaggatt gtgtggcgcg aaattcgcgg ccatcctagt
      481 gatcgtgttg gcagggtgcg ctaccggggg cttgacaagg cttgcgggcg acctgtttgt
      541 tcctcccgaa caggagataa aacttggtca gcagtttgcc gcggaaatac caaaagaata
      601 caagatctgc acggatgagg tgattcaagc gtacgttcgg gatgtgggga cccagatcgc
      661 tactgccgca tccaaagatc gttctgatgt cacctacacc ttcacggtta ttgacgaccc
      721 agaacaggtg aatgcttttg ctgttccggg cggatatatt tacgttttta ccggcctgat
      781 gctctttgca gagaatgagg ctgaactggc atcagttttg ggtcatgagg cgggacacat
      841 tgtcggccgg cattccgcca accagttggg tgctcgaatc gggctttcgt tggtcagttc
      901 ggtcgcactt ggacagaatc cgactctcct gcagcagatt gtggccagca tggcgaccac
      961 cgggtccgtc ctgaagttca gcagagacga tgagcgagag gctgattccc tggggcttaa
     1021 gtacacgatt caaggtggct atgacccaaa tgccatggtt tccttcatgc agcgactgca
     1081 caagaaatca ggggctggcg actccgaggt tgccgcgttt ctatccacac acccattgac
     1141 ctctgaacga attggcaatc ttcaggttga gatggcgaag tacgggaaga ccaagggtac
     1201 tctcaatgaa aaagccttca agcggattca ggaacagata aaggcgcttc ccaaggacgg
     1261 ctctgccacg tcaacggcca agtagttgga gtggagttcg ctccaagata aatgaggaaa
     1321 gggatcgaag ggattccagg ctggcggagt cagcagtctg gattttccca agtgcctctt
     1381 gggaggcctc tgagtagtac cttgccttct gaaacgcaaa gtccaaactg cccgtttttc
     1441 gtagatcttc cgcttccaat tcagcgataa ccgtgttgtc tcggcgctca gggcttttcg
     1501 ttgccagatg atgaatggtc gccagagttg tcttgccttg cgaaatatca gttcccaatg
     1561 tcttgccgga aaaggaatcc tcactcagaa aatccagaag gtcatcaaca atttggaagg
     1621 ccattccaaa ggaatggccg aaatcaccaa gtgcgtcaac gataggacgt tccgcctttc
     1681 caagcagacc accacagcgg gcgcacgatc ggaacagcga agcagttttt cgatctatga
     1741 ttcccaggta ctcttcctca gtgactgaag cagagcctga cgcattctgc tgagccatct
     1801 ctccaaggca gaccctgaca gcggatcccg agatatcctc aagacacgcc tgagaattcg
     1861 aacgagtcaa gaggttgaac gcaagagcca aaagcaggtc tccgccaaga accgcaaggg
     1921 aattgcccca gcgactggat gcactcggtt ggcctcgacg cagggatgcc ccatccagaa
     1981 cgtcatcatg catcagggaa gccgtgtgga tcatctcaca ggcaacggca acatccaccg
     2041 aagtgatcgg gtctactcca gtcaccttgg tgcacaggag tgtaatgcct gcgcggagcc
     2101 acttgccctg tgctgaagat atggtttcaa gcattctatc cagaaaatca gattgtgatg
     2161 atatgttacg gatcgcgaaa tcttcgagaa ggaactcgct cactcccgtt cgaagcatgg
     2221 actcgtactt tctctgtgct tttgcaagga cagccgccac cggtcgaaat acctctctga
     2281 gtctctctgc ctcattggtg gtggcctctg tcttctgggc atggaaaact gccatcgtca
     2341 agaatctccg gtcatcggtt tgtcgtgaag gtacatatac caacgggcgg gaaatgtcta
     2401 ccgtttcatc ctcagaggca gctgacgatg gagtcagacc atatgactga aaaaccgatg
     2461 aaagagacag cggactttca cttgaccctc ttcgggagtc aaggtacact cccaaaagta
     2521 tcgtgtgctg tggtggagtg cgtgttccag gagtttgtga gagtgatgaa gagcggaaag
     2581 aagaagacaa aagcccctca tgatcaagaa gttggctccg acagcggaga taccacttca
     2641 tcatcttcga gatctgatcc ggcatc
//
LOCUS       DAOQUC010000464         2664 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_45920_length_2664_cov_2.916826, whole genome shotgun sequence.
ACCESSION   DAOQUC010000464 DAOQUC010000000
VERSION     DAOQUC010000464.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2664
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45920_length_2664_cov_2.916826"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1088)
                     /locus_tag="PKH07_08820"
     CDS             complement(<1..1088)
                     /locus_tag="PKH07_08820"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNT35086.1"
                     /translation="MASQTPQVENRKCPAFLNHWSRQILLLFFITGFFLRVLHLAGMT
                     KAPDFHAPILDSLYHDLWATRFVQGQLPQEPYFRAPLYPTLLGCLYRFFGRDLFIPRI
                     FGIFLGLGAAGLVLAIGTRVFGRAVGILSAGILLFYWPLIYHETELLIPATLVFLLML
                     ALWNLTRALERPERGTRWLLTGVCFGLAAITRPNILLFMPVLAGWLVWREKARARAWL
                     AVTLLTVGSIITIAPVTLRNYFVGRDFVLIASQGGINFYIGNNPESDGFTAKTPLGYN
                     WHGDYEDSVALFAKRRAEKIVGHPMKASEISRFWFREGYQFWIESPAQALRLLARKLY
                     LFWNAYEIKNNKNMYFQKRYSPVLRLPL"
     gene            complement(1063..1509)
                     /locus_tag="PKH07_08825"
     CDS             complement(1063..1509)
                     /locus_tag="PKH07_08825"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HNT35087.1"
                     /translation="MSAANVSPVNELTFRICYADTDAGGVVYYGNYLRFLEQGRTALL
                     RQMGWKIEELHRDGIIFPVHELNVLYKSPATLGEEIRVRTHLVEINRFSLRFRTEIES
                     IQTDRILVQAQVTNACINEAGKIQKIPDAMREVLEKALNGKSDATS"
     gene            complement(1506..>2664)
                     /locus_tag="PKH07_08830"
     CDS             complement(1506..>2664)
                     /locus_tag="PKH07_08830"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNT35088.1"
                     /translation="EVATRLAQKHQVRLFVPDYDKYFPRGQVKGELNLEIERIPFNTW
                     SFTRWNVNRRFLKALEAYSPDKVIVTDGWYFKPPLIRALKDFKPIVRYYAYEGLCLHR
                     HGIFYRYKYGRICEKDYLLGGLKDFFVCSKCMLGFLKATRCSYFGQEYLFSGAWLPSY
                     RKVVEEALSCASTVIVYNRFIADRVRRYCDDVRIVPSGVDPDLFPELPQTGNHEPVIG
                     MVGRVADPLKGRDLLFKAIDILRKKRTDFEAWLTVTPDMGFIPAGYHLVPWQTQENLH
                     KVYRHLDICVVPSLWPEPFGIVAVEAMACQRPLVATRVGGLQEIVVDGETGFLVDPSD
                     PQMLADRLEQLLDDPELRRSMGLKGRERVLNEFTWDHIVQKHYLPLLEQNQ"
BASE COUNT          661 a          721 c          695 g          587 t
ORIGIN      
        1 aaaagaggca accgcaacac cggcgagtat ctcttctgga agtacatgtt cttgttgttc
       61 ttgatctcgt aggcattcca aaagagatag agttttctcg ccaggagacg cagtgcttgt
      121 gccggactct cgatccagaa ttggtatcct tcccgaaacc agaatcggga gatctctgag
      181 gctttcatgg gatgtccaac tatcttctcg gctcttcgtt ttgcgaacag tgccaccgag
      241 tcctcatagt ccccatgcca attgtagccg agcggcgtct tggcagtgaa gccatcggac
      301 tcgggattgt tgccgatgta gaagttgatg ccgccctggg atgcaatcag aacaaagtct
      361 cttcccacaa aatagtttct gagagtcact ggagcgatcg tgatgatgct gcccactgtc
      421 aacagtgtca cggcaagcca cgctcgcgcc cgcgccttct cacgccacac cagccaaccg
      481 gcaagcacgg gcatgaagag aaggatgttc ggtcgagtga tcgccgccag gccgaaacag
      541 actccagtca gcaaccaccg agttccgcgt tccggtcgct ccagggctcg cgtcaggttc
      601 cacaacgcca acatgagcaa aaagacaagc gttgccggaa tcaggagttc cgtttcatga
      661 tagataagcg gccagtagaa aagcagaatt cccgccgaga gaattcccac cgctctgcca
      721 aaaactcttg tgccgatggc aagcacaagt cctgcggcac ccaagcccag aaagatacca
      781 aagattctgg gaatgaagag atctcgtcca aagaatctgt acaagcaccc aaggagcgtc
      841 gggtaaagag gtgcccgaaa atagggttcc tgaggcaact gaccttgcac aaagcgtgtt
      901 gcccaaaggt catggtagag gctgtccaag atcggagcat gaaagtccgg tgctttggtc
      961 atgccggcca gatgaagaac gcggaggaaa aatcccgtga tgaaaaacaa cagcagaatc
     1021 tgcctcgacc agtgatttag aaaggcaggg cattttcggt tctcaacttg tggcgtctga
     1081 cttgccattg agcgcctttt ccagaacttc acgcatcgcg tccggaatct tctggatctt
     1141 gccggcctca ttgatgcagg cattcgtgac ttgtgcctgc accagaattc ggtcggtctg
     1201 aatgctctcg atctcagttc gaaatcgaag gctgaatcgg ttgatctcga ccaggtgagt
     1261 ccgaacccga atctcctcac ccaacgttgc aggcgacttg tagaggacat tgagctcatg
     1321 cactggaaag atgattccat cccggtgcag ttcctctatc ttccacccca tctgccgcaa
     1381 gagcgccgtt cgcccctgtt cgagaaagcg caggtagttc ccatagtaga caactccgcc
     1441 ggcgtctgtg tcggcgtagc agatccgaaa ggtgagttca ttaactggag aaacattcgc
     1501 agcgctcatt ggttctgctc caacagggga aggtaatgtt tttggacaat gtgatcccaa
     1561 gtgaattcat tcaaaacgcg ttccctgccc ttcagtccca tagaccgcct gagttcgggg
     1621 tcgtccagaa gttgttcgag ccggtctgca agcatttgtg gatcgctcgg atcaacgaga
     1681 aatccggtct ccccatctac aacgatctcc tgaagaccac caacccgggt tgccaccaac
     1741 ggtctttggc atgccatggc ttccaccgca acgatgccaa atggttcagg ccagagcgag
     1801 ggcacaacgc aaatgtcaag gtggcgatag actttgtgca ggttttcctg cgtttgccag
     1861 ggaaccaggt gatagcccgc agggatgaag cccatgtcgg gcgtgacggt cagccaggct
     1921 tcaaagtcag tgcgcttctt tcgcaaaatg tcaatggctt tgaaaagaag atctcttccc
     1981 ttgagtggat cagccacgcg ccccaccatc ccgataactg gttcatggtt tccagtctgg
     2041 ggcaattcgg gaaacagatc aggatccaca ccggaaggca cgattctcac atcgtcgcaa
     2101 taccgtcgga ctcggtcggc gatgaaacgg ttatagacga tgacggtact ggcacacgac
     2161 agggcttcct cgaccacttt cctgtagctg ggcaaccatg ctcctgagaa cagatactcc
     2221 tgtccgaagt aagagcatcg ggtcgctttg agaaagccca acatgcactt ggagcagaca
     2281 aagaagtcct tcaatccgcc gagcagatag tccttctcac aaatgcgacc atacttgtag
     2341 cggtagaaga tcccgtgtcg gtgaagacac agcccctcat aggcgtaata gcggacgatg
     2401 ggtttgaaat ccttcagtgc ccgaatcagg ggcggtttga agtaccatcc gtcggtgaca
     2461 ataactttgt ctggagagta cgcctcaagc gccttgagga atcggcgatt tacgttccaa
     2521 cgagtaaaac tccacgtgtt gaagggaatt ctctcgatct ccagattcag ttcgcctttg
     2581 acctgtcccc gtggaaaata cttgtcatag tcgggaacaa aaaggcgaac ctgatgcttt
     2641 tgggcaagtc gcgtggcaac ttcc
//
LOCUS       DAOQUC010000465         2646 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_46395_length_2646_cov_3.588576, whole genome shotgun sequence.
ACCESSION   DAOQUC010000465 DAOQUC010000000
VERSION     DAOQUC010000465.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2646
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46395_length_2646_cov_3.588576"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..376
                     /locus_tag="PKH07_08835"
     CDS             <1..376
                     /locus_tag="PKH07_08835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35089.1"
                     /translation="LIRGQEDQYIYNRNWAAATMEVRILTPEDALVLVDQDSVALIYN
                     CHREGWHTMHHRLKVSEREPGCDVLMDYIRDGARYFVVTDVAGFEYNRPVAEYVKSHA
                     KLIVDTPKHYLFEFFPLKESES"
     gene            373..1542
                     /gene="nagA"
                     /locus_tag="PKH07_08840"
     CDS             373..1542
                     /gene="nagA"
                     /locus_tag="PKH07_08840"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HNT35090.1"
                     /translation="MNGTERLIIKGGRIITSDQILDGFSILVEAGLIKAIEPSFPDQS
                     AAVIDAQSLYVSPGFIDMHIQGVLGRDVWEVETDGIEALCMLLPRFGCTAFLPTTNYD
                     EEHLPRLCARIASEHTGAQTLGLHLEGPFVHPQRRGALKVQTELPVSLENAKRVLDYS
                     RGLLKLITLAPELPGCLDVVSFFSAQGVLPSLGHTDSSFEIVQQAAARGLKHATHCFN
                     AMRPLNHREPGAVGAALLTDEISVQVVSDNHHLHPAMYDLIARVKPWEKIILVTDSIQ
                     AAGQPPGRYNSVGHGKEIFLKEDGTVRLADGTLAGSALTINQALQNFYEQVECSLPEA
                     VRLVTANPAKTLRVDHLMGSIKPGYQADLVLFDEAFNVRQTLVKGQTVFLFHPVS"
     gene            1685..1771
                     /locus_tag="PKH07_08845"
                     /pseudo
     CDS             1685..1771
                     /locus_tag="PKH07_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003112842.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
BASE COUNT          594 a          718 c          703 g          631 t
ORIGIN      
        1 cctgatccga ggacaggaag atcagtatat ctacaaccgt aactgggctg ccgcgacgat
       61 ggaagtccgt attctcaccc cggaagatgc tctcgtcctg gtagaccagg attccgttgc
      121 cttgatctat aattgccatc gtgaaggatg gcacaccatg catcaccgtc tgaaagtctc
      181 cgagcgagag cctggatgcg acgtgctgat ggactatatt cgcgacggag ccaggtactt
      241 cgttgtcacg gatgtcgcag gatttgagta caaccgtcct gtggcggaat atgttaagtc
      301 ccacgccaag ctcattgtgg atacccccaa acattacttg tttgagttct tccccctgaa
      361 ggagtcagaa agttgaacgg cacagaacgt ctcatcatca agggggggcg tatcatcacg
      421 agcgaccaaa ttcttgatgg cttcagcatc cttgttgagg caggtctcat caaggcgatc
      481 gaaccgtcct ttcccgatca aagcgcagcc gttattgatg ctcaatctct ctatgtttca
      541 cccggcttta ttgatatgca catccagggt gttctcggcc gcgatgtctg ggaagtggaa
      601 acggatggca tcgaagcgtt gtgcatgttg ctgccccggt tcggatgcac ggcatttcta
      661 cccaccacca actacgatga agaacatctg ccaaggctct gcgcgcggat tgcctccgaa
      721 cacacaggcg cgcagacact tggacttcat ctcgaaggac cttttgttca ccctcagcgc
      781 cgtggagcac tcaaagtgca gactgaactt cccgtgagtc tggaaaacgc caagcgcgtt
      841 ctggactatt ccagggggct cctgaaactc attacgctgg ctcctgaact accggggtgc
      901 cttgatgtcg tttccttctt cagtgctcag ggagtgcttc cttctctggg tcacacggac
      961 agttcctttg agatcgttca acaggcggct gcccggggac tgaagcacgc gacacactgc
     1021 ttcaacgcga tgcggccgct gaatcaccgg gagccgggag cggttggcgc cgcgcttctg
     1081 accgacgaaa tctccgtgca ggttgtctct gacaatcatc atctccaccc tgcaatgtac
     1141 gacctgatcg ctcgcgtcaa accctgggag aagatcatcc tggtcacaga ttcgattcag
     1201 gcagccggtc agcctccggg cagatacaat tccgtcggcc acggcaagga gatctttctc
     1261 aaagaggacg gaacggtgcg tctcgccgat ggtacgcttg ccggcagtgc cctgaccatt
     1321 aaccaggcac ttcaaaactt ctatgagcag gtagagtgtt cattgcctga ggcagtgcga
     1381 ctggtgacgg caaaccccgc caaaacgctc cgagttgacc atctgatggg cagcatcaaa
     1441 ccgggctatc aggcggatct tgtgctcttt gacgaggcct tcaatgtgag acagaccttg
     1501 gtcaagggac agaccgtctt cttgtttcac cccgtttcct agggcgtaag gtgacaaaga
     1561 gtgagtgcag actaccagcg cgattttctt ttgacaagga tagggcagac cagtaagata
     1621 acaaaggatc gatagagtcc gggagggatt ttgggatttc tctccaaaga aggaggtgag
     1681 aagtttgaag aacaaagctt tcacgcttat tgagcttctg attgttgttg ccatcattgg
     1741 aatcctggcg gcgattgcca ttccgaattt cctgatggcg caggtgcgag ccaaggtgtc
     1801 gcatggcgtt gcggagatgt cttcctgcgt cacggctctt gagtcgtatc gaatagacaa
     1861 cagccagtac ccgcccgccg gtgatcttcg ggcaggcctg aatccgagaa caacgggata
     1921 tcattcacgt ctcccaagtt atctgacgtc ccctgtagcc tacattaaga gtcttccttt
     1981 tgatccattc gtcgaagaac agagcacttg gtcaaatact tactatccgg tttcttccgg
     2041 tgtgggcaag agatatgtct actaccaaac gcagttcatg gtggaagaca acggcgatct
     2101 ttggggcggc cttcagcagt gggttggcgc atggctgttc tacggatatg gcccagacaa
     2161 aacgcctttc catggtgcaa gagccacctt gctcccttat gatccgacca atggcacgat
     2221 cagtctggga aatgtcatca agtgtcagaa gaaagcggac ggaattccgg tgcatccgac
     2281 aacaggcacc tatctgtggc cgtaagactt tttaccgcat tctgtcacaa ggcaacaacc
     2341 gacacctgag tattcgctca ggtgtcggtt ctcatttcac accgatgatc tgattcattg
     2401 cagacgcttg tcgcagctgg cagcacttac cagcatggcg gtatgcgttt gacaggaaag
     2461 tcctttccgg caggattgac aaggagaggc aactcttctt ccatcggctg gtgagaattc
     2521 tccgtcagtt tgctttcagc aattcggccc gcagtaggat caacagcggt ccttgtccct
     2581 ctccgggccg ggtgccctct cttctcatgc tccgactgac tcctctgggt gccagcctaa
     2641 gcggtc
//
LOCUS       DAOQUC010000466         2645 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_46421_length_2645_cov_3.140927, whole genome shotgun sequence.
ACCESSION   DAOQUC010000466 DAOQUC010000000
VERSION     DAOQUC010000466.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2645
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46421_length_2645_cov_3.140927"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..652
                     /locus_tag="PKH07_08850"
     CDS             <1..652
                     /locus_tag="PKH07_08850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35091.1"
                     /translation="TEGIPSDWRERVTVLLEMIYPVQRKRSDVESWLRDISTRAWTAM
                     PSDGVSAAEDARVQLGMETERPPLNSARSHIQQRINEEVFSLDRLRSDASGTLLDPQG
                     HEQDEFDFRYWCPRSFLPPMLETAADAAVPLCFVKMKTRPDGDSMSNAKADFYRAHLA
                     DYLAERGSMLIDLSQNPEIDFTWFSNESHISPQFRRRFTELFYEASKSIFGAEASP"
     gene            843..>2645
                     /locus_tag="PKH07_08855"
     CDS             843..>2645
                     /locus_tag="PKH07_08855"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HNT35092.1"
                     /translation="MRTRPSLFVVSILSVFVASPCFAGVVAFNSPEGDTFMSAKQAAD
                     NAESAVHAVLVGPTEEEIALGIWSAIPQKTRALSIDVFENGVLINLSAEAAEGLDDAT
                     WETMQRQFLWTMRPFGLEERVKVLIDGREVTELLSPTGPTLYSAPTDEERASGRSVEL
                     GDVGLSGKRITLCPGHGHYWNGSGWYFERPFICGYEQEDMRNMRVSKYLRVHLENDGA
                     YVQPTREFDMTRGTGPSGVPWWQESAYIYLKDQNYSCANVWANATGRCTDITGAGVTR
                     LNDTIRAKPLASNNDDRGNTDILIDIHSNAYQGECYGTSCPTGTDGMYTDDGGHEPWA
                     AQSISLAQKTQDGQIAAVQAAGFSYGHHGSTAALYGNFASTRMAQRPAALLEIGFHDS
                     CTTDAPKINDPFFQDCGAWGIYNGVCQYFGNTPTYGLYDSEYISDTIPGAMVAGEVRT
                     VQITFKNKSVRWDEAHAFRLGAVGDSDPFSATRHTIVGIIENNDTYTFSVQFAAPTTP
                     GTYTTDWRMVRDMFAWFGQTLTKEVTVSGSGATATPTSTPTQTPLPDPRFYNALQNGG
                     FENDFTNWVAGGSGATSYEILTSGAHSGIKACRFY"
BASE COUNT          643 a          680 c          727 g          595 t
ORIGIN      
        1 cacagagggc atcccgagcg actggcggga aagagtgacg gttcttctcg agatgatcta
       61 tccagttcaa aggaagcgtt cagatgttga gagctggctt cgagatatct cgactcgtgc
      121 ttggacagca atgccgtcgg atggtgtatc tgcggcagaa gatgcccgcg tacaattggg
      181 catggaaacg gaacgtccac cactgaactc agcacgatct catattcagc aacgcatcaa
      241 cgaagaagtc ttctccctgg acagacttcg ttcagacgct tctggcactc tgctcgatcc
      301 ccaggggcat gagcaagatg agttcgattt ccggtattgg tgtccccgat cctttcttcc
      361 gccaatgctt gagacggcag cggatgctgc agtcccactc tgtttcgtca agatgaagac
      421 gcgaccagat ggcgactcga tgtcaaacgc gaaggcagat ttctaccgtg ctcacttggc
      481 ggattatctt gcggaaaggg ggagcatgtt gattgatctg tcccagaatc cggagataga
      541 cttcacttgg ttcagcaatg agtcccacat cagccctcag ttccgacgaa ggtttaccga
      601 acttttctat gaggcgtcca agagcatctt cggggcggaa gcatcgcctt gatggctcta
      661 ccgaatctct acactcacta tccaaagatc atacgtgtgc atatactgat catcctaaca
      721 tactgataaa tgagaagata ttaaggggtg tcattttctg ttgaaacggt aaacgtttac
      781 ctatatcttc aaagtgaagt gtgtctctgg tcgggggaag caaggaaagg aaggccttgc
      841 cgatgcgaac cagaccttcg ctctttgttg tttccattct ctctgtgttt gttgcctccc
      901 catgtttcgc cggagtcgtt gcgttcaaca gccccgaagg cgataccttc atgtctgcaa
      961 agcaagcagc agacaatgct gaatctgctg ttcatgcagt gctcgtagga ccgaccgaag
     1021 aggagattgc cttggggatc tggtctgcaa taccccaaaa aacgagagcc ctgagcattg
     1081 atgtattcga aaacggcgtc ctcatcaacc tctctgctga agccgctgag ggtttggatg
     1141 acgcaacctg ggagacgatg cagaggcagt tcttgtggac aatgagacct tttggattgg
     1201 aagagagagt caaagtgctc attgacggca gagaggtcac cgagttgctc tcacccaccg
     1261 gccccacact gtactccgct ccaacggatg aggagcgtgc gtcaggacga tctgtcgaac
     1321 tgggcgatgt tggcctctcg ggcaagcgaa tcacgctgtg tccaggccat gggcactact
     1381 ggaacggaag tggctggtat tttgagcgtc cctttatatg cggctacgag caagaggaca
     1441 tgcgaaatat gcgtgtgtcc aagtatctca gagtccatct tgagaatgat ggtgcctatg
     1501 ttcagccgac gcgtgagttc gacatgactc gaggcacagg cccttcagga gtgccttggt
     1561 ggcaggagag cgcctacatc tatctgaagg atcagaacta cagttgcgca aatgtctggg
     1621 caaatgcaac aggacgatgc accgacatta ccggtgcagg tgtgacccgc ttgaatgaca
     1681 cgattcgtgc caaaccgctg gcgagcaata acgacgatcg gggcaacaca gatatcctga
     1741 tcgatatcca cagcaacgcc taccaggggg aatgttacgg gacaagttgt ccgactggaa
     1801 cggacggtat gtataccgac gacggcggtc acgaaccctg ggcggcacaa agcatcagcc
     1861 tcgcccagaa gacacaggac ggtcagattg cggcggttca ggctgcaggt ttcagctatg
     1921 gacaccacgg cagcaccgca gcactgtatg gcaacttcgc ctcaactcga atggcacagc
     1981 ggcctgcggc gctcttggag ataggcttcc acgacagttg cacgacggat gctcccaaga
     2041 tcaatgatcc ctttttccag gattgcgggg cctggggcat ctataatggc gtctgccagt
     2101 attttggaaa tacacctacg tatggactat atgattccga gtacatatcg gataccatcc
     2161 ccggcgcgat ggtcgccgga gaggttcgaa cggttcagat cactttcaag aacaaatctg
     2221 tgcgttggga cgaggcgcat gcttttcggc ttggagcggt tggggacagc gatccctttt
     2281 ccgcaacccg ccatacgata gttgggatca ttgagaacaa cgacacttac acattcagcg
     2341 tccagtttgc cgcaccaact acaccgggaa cctacacaac ggattggaga atggtgcgtg
     2401 atatgtttgc ctggtttgga cagaccctga ccaaagaggt cactgtttca ggcagtggcg
     2461 caacggcaac cccgacaagt accccgacac aaacacctct gccggatcca cgtttctaca
     2521 atgcgctcca aaacggtgga tttgagaatg acttcacgaa ttgggttgcc ggcgggagtg
     2581 gcgcgacgag ttatgagatt ctcacaagtg gtgcccactc ggggatcaag gcatgccgat
     2641 tctat
//
LOCUS       DAOQUC010000467         2639 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_46586_length_2639_cov_3.263932, whole genome shotgun sequence.
ACCESSION   DAOQUC010000467 DAOQUC010000000
VERSION     DAOQUC010000467.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2639
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46586_length_2639_cov_3.263932"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1097)
                     /locus_tag="PKH07_08860"
     CDS             complement(<1..1097)
                     /locus_tag="PKH07_08860"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase N-terminal
                     domain-containing protein"
                     /protein_id="HNT35093.1"
                     /translation="MKRILFVLCFGVLMTGFLLGTSASAQEGIARGIVFNDQNNNGVR
                     DAHEKGLRGVGVSNGTVVVQTDGEGKYELPVQGEETCLFVIKPTNWTPPLNDSNLMRF
                     HYFHKPKGSQGLKVAGLPPSGDLPDSVDFPLIRRIEPTRFKVLMLGDPQTTNSTEVSY
                     YARDVLAELVGTDAAFGVTLGDIVNDRPLMFEDNVAAVATVGVPWHHVIGNHDRNYDA
                     QDYDSYDDTYNRVVGPSHYSFNWGGVHFIVLNNVHSLGKYDYEGKLTQAQLDFVKHDL
                     AVVPDKQLIVLMMHIPLNETRNREEIFRLIEKRPYTFSISAHNHNTKHRFFEQAQGWQ
                     GTKPHHHFVSPTSCGSWWMGVPDEVGIPHAM"
     gene            complement(1129..>2639)
                     /gene="galK"
                     /locus_tag="PKH07_08865"
     CDS             complement(1129..>2639)
                     /gene="galK"
                     /locus_tag="PKH07_08865"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HNT35094.1"
                     /translation="LKMLNSAADLLKKLQTGDRTISQRIEEVYGDDRSERSVQFERLR
                     LLLQCHAEEHGASSPVQVFHAPGRVNLIGEHTDYNGLPVMPVAIGRDILCAASPRQDG
                     QMVVQNVNTRCGAFECPVASVPRPFEMGHWGNYVKAALQGLFDDGREYGVHADKFTGC
                     SFLIEGNVPSAAGLSSSSALVVVSALALLGVHGCEIPKPNLAQILARAEHFVGTQGGG
                     MDQTISLLGQRDHALKIDFNPFGYEPVPVPANVRIVVSNSLVKAAKTGDARAAYNRRV
                     VECRTAAALLDKAVRERISPETQIRLLGDFTPQRTGVSESDLDRIAEDTIPKTPLSIS
                     EAAKRLSVSESEYQKRCCTLRSGEILQPPSEGFQVWKRYAHVVSEGRRVESAMQALKA
                     GDLEQVGRLMLESHASCRDLYEISCDALDFLVSLAQSSGALGARLTGAGFGGCTVSLV
                     RAEQVEAFISTIHREYYEGYLQKNHPDICKYMPRVDSCLFAVRPAEGAGRIL"
BASE COUNT          639 a          748 c          653 g          599 t
ORIGIN      
        1 atcatcgcgt ggggaatacc gacctcgtcc ggtactccca tccaccagga accacaactg
       61 gtcggactaa cgaaatgatg atgaggcttt gttccctgcc acccctgcgc ctgttcaaag
      121 aaacgatgct tggtgttgtg attgtgcgcc gagatggaaa aagtgtacgg tctcttctcg
      181 atcagccgga agatttcttc ccgattcctg gtttcgttca aaggaatgtg catcatcaat
      241 acgatgagtt gcttgtcggg cacaacagcc agatcgtgtt ttacaaagtc caactgggct
      301 tgagtcagtt tcccttcgta atcatatttg cccagggaat gaacattgtt gagaacgatg
      361 aaatggactc ctccccagtt gaaactgtaa tgggaagggc caacaacgcg attgtaggtg
      421 tcatcatagg aatcatagtc ctgcgcatcg tagttgcgat catggtttcc aatgacatga
      481 tgccagggaa cgccaacggt cgccaccgcc gctacattgt cttcgaacat cagcggcctg
      541 tcattgacaa tgtcgcccag cgtgacacca aacgctgcgt ccgtacccac caactcggcc
      601 aacacatcac gggcgtaata ggagacttcc gttgaattgg tcgtctgcgg atcgccaagc
      661 atcagcactt tgaaccgtgt cggctcgatc cttcggatga gcggaaagtc aactgagtcc
      721 ggcaaatcgc ccgacggtgg aagtccagca accttgagtc cctgtgatcc ctttggtttg
      781 tgaaaatagt ggaagcgcat gagattgctg tcattcagcg gtggagtcca attagtcggt
      841 ttgatgacaa aaagacaggt ctcttcgccc tgcacaggca gctcatactt tccttcgcca
      901 tccgtctgta cgaccaccgt accgttggaa acaccaacgc ctcggagtcc tttctcatgg
      961 gcatcgcgaa cgccattgtt gttctgatca ttgaagacga tgcctcgggc gatcccttcc
     1021 tgtgctgacg cggatgtccc cagaagaaaa cccgtcatca gaacgccaaa acacaaaacg
     1081 aaaagaatac gtttcattct tctcccttcc tttcctgtct cgaacggatc aaagaatcct
     1141 tcctgcgccc tctgcggggc gaaccgcaaa gagacaggaa tcaacgcgag gcatgtactt
     1201 gcagatgtcc ggatggttct tctgaagata tccctcgtag tattctcgat gaatggtgct
     1261 gatgaaggct tcgacttgtt ctgccctcac cagactgacg gtacagccgc caaaacccgc
     1321 tcctgtaaga cgggctccca gagctcccga tgactgtgcc agtgaaacca aaaagtccaa
     1381 tgcgtcgcaa ctgatctcat agaggtcgcg acaacttgca tgagactcaa gcatgaggcg
     1441 tcccacctgt tcaaggtctc ccgctttcaa agcctgcatc gccgattcaa cacgccgacc
     1501 ttcgctgaca acgtgggcat accgcttcca aacctgaaaa ccttcggatg gcggctggag
     1561 gatttccccc gaccgaagag tgcagcagcg cttctgatat tcactctcgg acacactcag
     1621 gcgtttggcc gcttcagaga tggaaagcgg tgtcttggga atagtgtctt cagcgattcg
     1681 atccaagtcg gactcggaca cacccgttcg ttgtggggta aaatcaccca agagacgaat
     1741 ctgagtttcg ggagaaatgc gctctctgac ggccttatcc aaaagcgccg cagcggttcg
     1801 acattccacc acacgcctgt tgtatgcggc acgagcatct cctgttttgg ccgctttgac
     1861 cagactgttg ctgacaacaa ttcggacatt cgctggcact ggaaccggct catatccaaa
     1921 ggggttgaag tcaatcttga gcgcatgatc ccgctggcca agaagagaaa tggtctgatc
     1981 cataccgccg ccctgcgttc caacaaaatg ttcagcacgt gcgagaatct gtgcaagatt
     2041 cggcttgggg atctcacaac catgaacacc cagcagggcg agggcggaca caaccaccaa
     2101 agcggaagac gaactcaatc ccgccgcgct tggaacattt ccttcaatga gaaagctgca
     2161 accggtgaac ttgtccgcgt gaacaccgta ctctctgccg tcatcgaaaa gcccttgtaa
     2221 ggcggccttg acatagtttc cccagtgtcc catttcgaaa ggtctcggaa cagaggccac
     2281 cggacactca aacgcgccac agcgcgtgtt cacgttctga acaaccatct gcccatcctg
     2341 tcgtggagag gcggcacaga gaatgtcccg accgatggca acaggcatca ctggaagacc
     2401 attgtaatcg gtatgctctc cgatcaggtt gacccgacca ggtgcatgaa agacctgtac
     2461 gggacttgac gctccgtgct cttcagcatg gcattgcagc agaagtctca gtctttcaaa
     2521 ctgcacagac cgctcggatc tgtcgtctcc atagacttcc tcaatcctct gggagatagt
     2581 cctatccccc gtctgaagtt tcttcaacaa gtctgccgcg gagttcagca ttttcagtg
//
LOCUS       DAOQUC010000468         2638 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_46619_length_2638_cov_2.917151, whole genome shotgun sequence.
ACCESSION   DAOQUC010000468 DAOQUC010000000
VERSION     DAOQUC010000468.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2638
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46619_length_2638_cov_2.917151"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..454
                     /locus_tag="PKH07_08870"
     CDS             <1..454
                     /locus_tag="PKH07_08870"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HNT35095.1"
                     /translation="TILGEGRSSRLYRILQEQKRLVSDIDAFEEMFRRGGFLAVYACF
                     DKSRTKAVTRAIWEVFDSLRDSLVQPEELAKVKTMLCTDFLFANERISSMASTLGDYQ
                     INHCLEQASEYTDRMQSVTASQVQDIIRRYCRPESQVLGLVVPKKERN"
     gene            461..1783
                     /locus_tag="PKH07_08875"
     CDS             461..1783
                     /locus_tag="PKH07_08875"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNT35096.1"
                     /translation="MLPKMTTRETLLSNGLKLLVRENTENEIVAIRILFKTGNRYETA
                     SNYGISNLAFRMLLKGTESRSAEQIADDLDSIGAHLEVGVEKDAGGLMLQCTAPVLDE
                     ALEILWDVANRSVFPADRLRWEKKRVKMEILEDEDSPMTYTFRRFWENLYGSHPYSAL
                     SKGYPETLSRMTKARVKKHYLTCGSSSGIVATIVGHVNATRITQWFESHFGTRQLDSK
                     RPSEPDDVVLLACSGSEKHTQHQRMVEAENLVLGYLAPCVLSSDFPAMKVLDGVLGGS
                     MDSRLFVEVREKRGLAYSVGSTYIPRRGPGALAIYLGTEPSNHEEALSQCLLQVEQLQ
                     NDLVPEDELRRTIQYICGLHIMGQESNMGQAEMLASSTFFDLGSGYISEYPALIEAVT
                     AEDVRHVAQKYLTDYASAVTTPCPSEPSSTQGDIKRPMLDSEDLDALE"
     gene            1799..>2638
                     /locus_tag="PKH07_08880"
     CDS             1799..>2638
                     /locus_tag="PKH07_08880"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNT35097.1"
                     /translation="MASPNTMTSRERVIRSLCHQTPDRVPMNYHADGNFSKQLRARFG
                     LQGNQARNCWLTGNEDPRLLEALGCDLRMLVPDYVGPEIKTNPDGSFTSVFGEHLLIR
                     ENEIGAAFFEYPFLPLADAKSVEQVNAHPWPKAEWFDFACIKDQLDQWDEYAIIGGDM
                     GTLDCMNRCLTLFGYERVMIGMAERDPVILTAFERIITFYNEYLKRLTDAAGDRIDII
                     WLGEDLGTQKGPRISASMFRELIRPLWESVLRDIHSRGYFVMQHSCGSTRAFYPDFIE
                     MGID"
BASE COUNT          628 a          687 c          705 g          618 t
ORIGIN      
        1 aacaattctg ggagaggggc gcagctcgcg tctgtatcgg attcttcagg aacaaaagcg
       61 ccttgtctcc gacattgatg cttttgaaga gatgtttcgt cgagggggct tccttgcagt
      121 ctatgcctgt tttgacaaat ccagaacgaa agccgtcact cgagccatct gggaggtttt
      181 tgacagcctg agggattctc ttgtccagcc cgaggaactg gcgaaggtga agaccatgct
      241 ctgcacggac tttctttttg ccaacgaacg aatatcgtca atggcgagca cactgggcga
      301 ctatcagata aatcattgcc tcgaacaggc gtccgagtac actgacagga tgcagtcggt
      361 cacggcatct caggttcagg acataatcag acgctactgt cgtcctgaat cacaggtcct
      421 gggtctcgtt gtgcccaaga aggaaaggaa ctgagaaccg atgttgccga aaatgacaac
      481 ccgggaaaca ctcctttcca acggattgaa actgcttgtt cgcgagaaca cagagaacga
      541 aattgtagcg attcggatcc tcttcaagac gggaaaccgc tatgaaacag cctccaacta
      601 tggaatctcg aatctcgctt tccggatgct cctgaaggga acggaaagcc gcagtgctga
      661 acagattgcc gatgatcttg attcgatagg ggcacatctc gaggtggggg tcgagaaaga
      721 tgcgggcggg ctaatgctcc aatgcactgc tccggtgctg gatgaggctc tggagattct
      781 ttgggatgtc gccaaccggt cggtcttccc agccgacagg ctgcgatggg aaaagaagcg
      841 cgtgaagatg gaaatcctcg aagatgagga ttctccgatg acctatacct ttcgacgttt
      901 ctgggaaaac ctctatggca gccatcccta ttcggctctc agcaaaggct atcccgagac
      961 gctatccaga atgacaaagg ctcgggtcaa aaagcactat ctcacatgtg gatcatcctc
     1021 aggcatcgtt gcgactattg tcggacatgt caacgcgacc aggatcaccc agtggttcga
     1081 gagtcatttc ggaacacggc agctggactc caaacgtcct tcggaacccg atgacgttgt
     1141 gttgttggca tgttctggtt cggaaaagca cacccaacac cagcgaatgg tcgaagcgga
     1201 gaatctcgtt ctcggctacc tcgcaccctg tgtgctgtcc tctgattttc ctgccatgaa
     1261 ggtgctggat ggtgttctgg gtggttcaat ggattcgcgg ctgtttgtgg aagttcgcga
     1321 aaagagggga ctggcttact cagtcggctc aacctacatc ccccgtagag gtcccggtgc
     1381 cttggcgatc tatcttggaa cagagccatc caatcacgaa gaggctctgt cccaatgcct
     1441 gctccaagtc gagcaactgc agaatgacct tgtgccggaa gatgaactgc gacgcacgat
     1501 ccaatacatc tgtggactcc acatcatggg tcaggagagc aatatgggtc aggctgagat
     1561 gctggcctca agcactttct tcgatcttgg ctcgggttat atttcggagt atcctgcact
     1621 cattgaagca gtgaccgcag aggatgtgcg tcatgtcgcc cagaaatatc tgactgacta
     1681 cgcctcagca gtcacaacgc catgtccttc agagccatct tcgacacagg gagatatcaa
     1741 acgccctatg ctcgattctg aggatctgga cgccctcgaa tgaaagggag gctttcctat
     1801 ggcaagtccc aacaccatga catccagaga gcgggtcatt cggtcgctgt gccatcaaac
     1861 cccggatcgc gttccgatga actatcatgc cgacggcaac ttctccaaac aacttcgagc
     1921 ccgtttcggc ctgcaaggaa atcaggccag aaattgctgg ctcacgggaa acgaagaccc
     1981 tcgcctgctg gaagccctcg gttgcgatct ccgaatgctg gttcctgact acgtgggacc
     2041 ggagatcaaa accaatcctg atggtagttt cacatctgtc tttggagaac atctattgat
     2101 tcgggagaac gagatcggcg ctgctttttt tgagtatccc ttcctgcctc tcgccgatgc
     2161 caagtcagtg gagcaagtca atgcacatcc ttggccaaaa gcagaatggt ttgatttcgc
     2221 ctgtatcaag gatcaactcg accaatggga tgaatacgcc atcatcggtg gagatatggg
     2281 caccctggat tgtatgaacc gatgccttac cctctttggc tacgagcgcg tgatgatcgg
     2341 gatggcagag cgagatcccg tcatcctgac tgccttcgaa cggatcatta cgttctacaa
     2401 tgagtatctc aagcgactca cggatgccgc gggcgatcga attgatatca tctggctggg
     2461 agaggatctc ggaacgcaga aggggcctcg tatcagcgct tcgatgttcc gcgagttgat
     2521 ccgtccactt tgggagtctg tcttgcgaga cattcactcc cgagggtatt tcgtcatgca
     2581 gcattcatgc ggttccaccc gcgcttttta ccctgatttc atcgagatgg gcattgac
//
LOCUS       DAOQUC010000469         2635 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_46703_length_2635_cov_3.456977, whole genome shotgun sequence.
ACCESSION   DAOQUC010000469 DAOQUC010000000
VERSION     DAOQUC010000469.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2635
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46703_length_2635_cov_3.456977"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..526
                     /locus_tag="PKH07_08885"
     CDS             <1..526
                     /locus_tag="PKH07_08885"
                     /inference="COORDINATES: protein motif:HMM:NF021507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HNT35098.1"
                     /translation="SDHVYASRHPRTAVAYNDTHVFFVVCDGRSAESRGMSSQELSNF
                     LMSTLGVTNALNLDGGGSSTMVVKGTVVNVPSDGSERAVGNGLMMVNLKPKTQSQTFQ
                     TGQRIAAASGVDVRLGPGTNYGSLKTLTETTLVTVEPHSLNGIHATADHWWKVDCQDV
                     VGWLRETGLTATGD"
     gene            674..1342
                     /locus_tag="PKH07_08890"
     CDS             674..1342
                     /locus_tag="PKH07_08890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35099.1"
                     /translation="MNKPGSVMLILELPEPPLRQLADRYASEAEALGIQVLEVPLERA
                     ARESFDTIKSRIESNGLLAAIIHVAVGWPLSECETKPCLVSPRDHLALWDFAEYSPRD
                     AGGSVAFLDISHPYDEALLHMAEQTAREMQMTWASEVIASHPCPWILTPTEKAILQRT
                     GCQTVTRIVPLTAKLARAFGIPVLGILALPEWKQDATFTDIGESLEGFLDRLFENIRC
                     VGDL"
     gene            1339..>2635
                     /locus_tag="PKH07_08895"
     CDS             1339..>2635
                     /locus_tag="PKH07_08895"
                     /inference="COORDINATES: protein motif:HMM:NF016623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HNT35100.1"
                     /translation="MMRLLMKPWSQMSRGLRVVLATSALIGLLIAPVAALGQYQVVFD
                     VEDPCFEIYSGAWTLEKTGTYLGSFYYAISGPGTGAGTARWTAEGLPAGSYLVECWAN
                     NGNYASDARYQVICSDGAHSVSLNMNYVTAGWYSLGTYSIDRVCVVNVSDYWTGSGTL
                     LSVDALRFTYQGTVPAPPATDIVPHIGVCIDDAGSVNPTDPAQPIYSMLRLPFPLTIA
                     VMPQRTYTTQTANEVYARGSEVILHQPMAAITVPDPGAGGITSSMTLEQVRTVVSTNL
                     NAVPHCIGMNNHMGSLITQQTDKMQVCMEELKARDQFFYDSRTITTSVGYDVAKNNGL
                     LTAERDLFIDGNSKEESKALIRSLALRALHAPYVTHCAIGHVRANTAAALQEMVSELE
                     AMGVEIWPISRCMTQIIETDNQPSGCSVSLEGAWIADTPD"
BASE COUNT          576 a          701 c          757 g          601 t
ORIGIN      
        1 gtctgaccat gtctacgcct ctcgtcatcc aagaacagcc gttgcctaca acgacacaca
       61 cgttttcttt gtcgtttgtg atggacggtc agcggagagt cgaggcatgt ccagtcagga
      121 gttgagcaac ttcctcatga gcacattggg cgttaccaat gccctcaatc ttgatggggg
      181 aggttcttcc accatggtgg taaagggaac cgtggtcaac gttccctcgg atggctccga
      241 acgggcggtg ggtaacggtc tgatgatggt caacctgaaa cccaagaccc aatcgcagac
      301 ttttcagaca gggcagagaa ttgctgctgc ttcgggtgtg gacgtacgac tcgggcctgg
      361 cacaaactac ggctcactga agactctcac ggagacgacg cttgtcaccg ttgagccaca
      421 ttccctgaat ggcatccacg ctacagcaga ccattggtgg aaggtggact gccaagatgt
      481 ggttggttgg ctgagagaaa ccggactcac ggcaaccgga gactgaccag gcactgactg
      541 gctcttggtg acgcagagtc cgagcggcgt gtctcatttg ttgagtaagc gtccaaggga
      601 gtgggcatag cccacacgcc tgcaggtgca acctgctttt ggaatgtggt ttcctgggag
      661 aagaggatac accatgaaca agcctggttc tgtcatgttg attcttgagt tgcctgagcc
      721 gcctcttcgt cagcttgccg accggtatgc atcagaggcc gaagccctcg gcattcaggt
      781 tctggaagta cctctcgagc gtgctgccag ggagtcgttt gacaccatca agagccgaat
      841 cgagtccaat ggccttcttg cggcaattat ccacgttgcg gtgggttggc cgctttcgga
      901 atgtgaaacg aagccttgtc tggtttctcc aagggatcat ctcgcgctct gggactttgc
      961 cgagtattct ccgagagatg cgggaggttc ggtcgcattc ctggacatca gtcatcccta
     1021 cgatgaggct ctgctccaca tggcagagca aacggcgcga gagatgcaga tgacctgggc
     1081 ttcagaggtg attgccagcc atccctgtcc ctggattctc acgccgaccg aaaaggcgat
     1141 actgcaacgc accggatgcc agacagtgac acggatcgtg ccgctgacgg cgaaactggc
     1201 tcgcgcgttc ggcattcccg tgttgggaat tcttgccttg cctgaatgga aacaagacgc
     1261 tacctttacg gatattggag agtcgctgga aggctttttg gatagactct ttgaaaatat
     1321 ccgatgcgta ggagatctat gatgcgtttg ttgatgaagc cctggagtca aatgagccgg
     1381 ggactgaggg ttgtgctggc aacgagtgcg ttgattggat tgctcatcgc gccggtggcc
     1441 gcgctcggtc agtatcaggt tgtatttgat gtggaggatc catgttttga aatctacagc
     1501 ggcgcatgga cgctggaaaa gacgggaact tatctcggct ctttctacta tgccatcagc
     1561 gggccgggaa caggtgccgg aaccgcgaga tggactgccg agggactgcc cgcaggttcc
     1621 tatctggtcg agtgttgggc gaacaacggc aactatgctt ccgatgctcg ttatcaagtc
     1681 atctgctccg atggagcgca cagtgtctcc ctgaatatga actacgtcac ggcaggatgg
     1741 tattccctcg gaacgtacag cattgatcgc gtctgtgtcg tcaatgtcag cgattactgg
     1801 acgggatccg gaacgctcct gagcgtggac gcactgcgtt tcacctatca gggcaccgtt
     1861 ccagcgccac cagcgacgga tatcgttccg cacattggtg tgtgcattga tgacgcaggc
     1921 agcgtgaatc cgaccgatcc ggcgcagcca atctactcca tgcttcgcct tcccttccct
     1981 ctgactattg ccgtcatgcc gcagcgaacg tacacaacac agactgccaa tgaggtctac
     2041 gcccgtggtt ccgaagtgat tcttcaccag cccatggctg ccatcacggt tcccgatcca
     2101 ggagcaggtg ggattaccag ttccatgacc ctggagcagg ttcgaactgt cgtctccacc
     2161 aatcttaacg ctgttcccca ttgtatcggg atgaataacc acatgggctc actcatcaca
     2221 caacagaccg acaagatgca ggtgtgtatg gaagagctga aagcacgcga ccagtttttc
     2281 tacgacagcc gaacgattac gacatcggtc ggctacgacg ttgccaagaa caacgggctt
     2341 ctgaccgcag agcgcgattt gtttattgat gggaactcaa aggaagagtc caaggcgctg
     2401 attcggagcc tcgcccttcg agcgctgcac gctccttatg tgacgcactg tgcgattggg
     2461 catgttcggg ccaacactgc cgccgcgctt caggagatgg tctcggaact ggaagcgatg
     2521 ggtgtcgaga tctggcctat ctctcgctgc atgacacaga ttatcgaaac agacaaccag
     2581 ccatccggtt gttcagtaag tctcgaagga gcatggattg ctgatacgcc agatg
//
LOCUS       DAOQUC010000470         2635 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_46712_length_2635_cov_2.905039, whole genome shotgun sequence.
ACCESSION   DAOQUC010000470 DAOQUC010000000
VERSION     DAOQUC010000470.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2635
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46712_length_2635_cov_2.905039"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..864
                     /locus_tag="PKH07_08900"
     CDS             <1..864
                     /locus_tag="PKH07_08900"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HNT35101.1"
                     /translation="TRTGQFSIGFRRGGSEWQKDLGALVRWTVESGLGAIDLGRDADE
                     KASVVLDAGLRIGSVDLKAWQPMLSPDKGKRDEAVAQNAEYIKACAKHGAMNHFLVML
                     PEKPELPRSENFGYMVESFAQLVPVLEHNNAKLVIEGWPGAGALCCTPEGYRSFFEKL
                     PSMALGINYDPSHLIRMGIDPIRFLHEFGDRVYHIHGKDTEIFDENLYEFGTEQPPTF
                     AKGFRFGSIAWRYTIPGHGRMRWVQGFKILEEKGYSGCVCIELEDGNFNGSEQTEMAG
                     IMLGARFLHGC"
     gene            1142..1909
                     /gene="hisF"
                     /locus_tag="PKH07_08905"
     CDS             1142..1909
                     /gene="hisF"
                     /locus_tag="PKH07_08905"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HNT35102.1"
                     /translation="MVDYRRVIPCLDVKDGRLVKGVNFVALKDVGDPAEAGAAYSAAG
                     ADELVFLDITATVECRQTLVEAVKRTLAKINIPLTVGGGIASLQDVKRLMDIGVSKVS
                     MNTAAVYNPDLIKEAVYEYGKDRVVVAIDTRKNSELPSGFEVMVKGGKKPTGMDAVEW
                     AKGAEECGAGALLPTSMDADGTQAGYDIPMTRAIADAVKIPVIASGGAGKLEHLAEAV
                     TEGHADAVLVASIAHFGTFTIRQMKEFLKSKGIPVKL"
     gene            1998..2348
                     /locus_tag="PKH07_08910"
     CDS             1998..2348
                     /locus_tag="PKH07_08910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35103.1"
                     /translation="MVWGDGIDQLPFETVLYLALMVVLLGMGVIWFWLIARLYRRLER
                     DHPEKYLAMGKPTLFWRSSLVSSFDLLRFIFLQEHRELNDAHLSRLRDRMRLLLLTYT
                     LLLAVELFLLARIG"
BASE COUNT          578 a          671 c          764 g          622 t
ORIGIN      
        1 acgcgtacgg gacagttcag tatcggcttc cgacgaggcg gaagcgaatg gcagaaggat
       61 ttgggggctc ttgtccgatg gacagtcgag agcggtctgg gagcaattga tctcggtcgg
      121 gatgccgatg agaaagcatc cgtggtcttg gatgccggtc tgcgaatcgg ctctgtggat
      181 ctcaaggcat ggcagccgat gctctcaccc gacaaaggca agcgagatga ggcggtggca
      241 cagaacgccg agtacatcaa ggcctgcgcg aaacacggcg ctatgaacca ttttctcgtc
      301 atgctgcccg aaaagccgga actccccaga tcggaaaact ttggctacat ggtcgagagc
      361 ttcgctcagt tggttcccgt tctggagcac aacaatgcca aactggtcat tgaaggctgg
      421 cctggagccg gagcgctgtg ttgtacgccg gaaggctatc gttccttctt cgagaaactg
      481 ccatccatgg cgcttggcat caattacgat ccgtcgcatc ttattcgcat gggcattgac
      541 ccaattcgtt tccttcatga atttggtgac cgtgtctatc acatccacgg caaggataca
      601 gagatctttg acgagaacct ctatgaattc ggcacagagc aaccgccgac ttttgcgaag
      661 gggttccgtt tcggatccat cgcctggcgg tacaccatcc ctggccacgg cagaatgcgc
      721 tgggttcagg gattcaagat tcttgaggaa aaggggtatt ccggctgcgt ctgcatcgaa
      781 ttggaggatg ggaatttcaa cggctctgag cagacggaga tggcaggcat tatgctcggc
      841 gcgcgttttc tgcacggctg ctgagcaggt tagcgcctgc gggcgggcta tgctcactcc
      901 catgttcggt gaatgagagt ctcctcgtca cagggagcag cgggtagcac ccatctgggt
      961 gtattctgga tttgtgtgct caggccagaa accagattcc tttggagtcc aggttttcga
     1021 gaatatgcag aatggctctc aaagtgggga cagcctgcgt tattcttgtt ttgcctgttg
     1081 aaaacgaaca cgtaagtctt taatatcgct acgagatttg aagaatacca aggaggtctc
     1141 aatggtggac tatagaagag taatcccatg tttggacgtg aaagacggcc ggctggtcaa
     1201 gggcgtcaac tttgttgccc tcaaggacgt aggtgatccc gcagaggccg gcgctgctta
     1261 cagtgccgcg ggcgcagacg aactggtatt cctggacatt acggcaacgg tcgaatgccg
     1321 ccagacactt gtcgaggcgg tcaagcgcac tctggcaaag atcaatatcc ccctcacggt
     1381 cggaggcggc atcgcaagcc tgcaagacgt caagaggctc atggacattg gagtctccaa
     1441 ggtctccatg aacaccgcag ccgtttacaa ccctgacttg atcaaggaag ccgtctacga
     1501 gtacggcaag gatcgggtcg ttgtcgccat tgatacccgc aagaattccg aacttccttc
     1561 tggttttgag gtcatggtca agggtggaaa gaaaccgacc ggaatggatg ctgtcgaatg
     1621 ggcgaaaggt gctgaggaat gcggcgccgg tgctcttctt cctacaagca tggatgcaga
     1681 tggaacccag gccggctacg atattcccat gactcgcgcc attgcggatg cggtcaagat
     1741 tcccgtcatc gcttccggtg gcgccggaaa actcgagcac cttgcagaag cggtgactga
     1801 aggacacgcg gacgctgttc tcgtggcttc cattgcccac tttggcacgt tcacgatccg
     1861 acagatgaaa gaattcctga agagcaaggg aattccagtc aagctgtagg atagcctact
     1921 atcattctgt cgctgtagaa tgctgcctag cctgaagcga aaactctgca caagtctggc
     1981 gcatgaggtg agggtgcatg gtttggggag atggaattga tcaactgccc tttgaaaccg
     2041 tcctctatct agcgttgatg gttgtcctct tgggtatggg cgtcatctgg ttctggttga
     2101 ttgcccggct ttaccggcgg ttggagagag atcacccgga gaagtatctg gcgatgggca
     2161 agccaacgtt attctggcgc agcagtctgg taagttcttt tgatctcttg agatttatct
     2221 tccttcaaga gcatcgcgaa ctgaacgatg cgcacctttc aagactccga gaccggatgc
     2281 gattgcttct tctgacctat accctattgc ttgctgtcga gttgtttctt ctggcaagga
     2341 ttgggtaagc cggtaggacg gggggactgg tttcatcctg cgcagcataa atgctgccct
     2401 acctcctgtc attccgaacg cagtgaggaa tctcgccggc gttcaaccac cggatcattg
     2461 gttcctacaa aaccatctac ttatactccg accaaccctg cacccccgtc gaaagcgttg
     2521 gcgttgctgt cggtgtgggt gtgtctgttg gcgtttgtgt cggcgtggcg acctggaatg
     2581 tcaccgaatc ctccgccaaa actgtcggcg tcggactgcc gttcacaatc tgcgc
//
LOCUS       DAOQUC010000471         2633 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_46761_length_2633_cov_3.411559, whole genome shotgun sequence.
ACCESSION   DAOQUC010000471 DAOQUC010000000
VERSION     DAOQUC010000471.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2633
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46761_length_2633_cov_3.411559"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..354)
                     /locus_tag="PKH07_08915"
     CDS             complement(<1..354)
                     /locus_tag="PKH07_08915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35104.1"
                     /translation="MAGCRVTRTWKSKKSVILVDTVRVTLSERVFEFHDGGYVMRRFL
                     IFGALVAICLSSWADSPEFRGVYVPYWEMNTQAKCDTIIAKILARNINSVYIQVRGRG
                     DAFYFPNRESTVYPNP"
     gene            complement(565..1749)
                     /locus_tag="PKH07_08920"
     CDS             complement(565..1749)
                     /locus_tag="PKH07_08920"
                     /inference="COORDINATES: protein motif:HMM:TIGR03942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HNT35105.1"
                     /translation="MLIKPVSAGCNLNCEYCFYRRVEHIYPKKPTERMPDAILEKLLR
                     EHMSFRLKQSVYGWQGGEPTLAGLDFFKKVVEFQKRFGAPGQVVGNSLQTNGILLDEK
                     WCAFLAEYKFLIGLSIDGPPDLHNLHRKNFGDEGTHEQVMNSARLMRRHGVEFNALTV
                     LTPAVEGRAKDIYRWFVSNEFNFMQFIPCCDLDPETGKPTDWFLTPEIYGQFLCDLFD
                     EWYPKDVGRISIRLFDAMIGYAVDRKMLTWCTFGDRCDDYLLVEYNGDIYPCDFFVYP
                     EWKLGSLTTGTLVEAAQSERRVEFARNKKVSEACQGCRFVQHCHGGCQKDRLLGKDGR
                     SVYCSAYQRFFAHTADAFRKLGDEVLEMRRQRTMQTHTPVGRNDPCPCGSGKKFKLCC
                     GK"
     gene            complement(1782..>2633)
                     /locus_tag="PKH07_08925"
     CDS             complement(1782..>2633)
                     /locus_tag="PKH07_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013566389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNT35106.1"
                     /translation="GVPGLAKTSLVQAIARVLALTFKRIQFTPDLMPSDITGTDILEV
                     DTATGERYFRFVKGPLFAQIILADEINRTPPKTQAALLEAMQEYSVTASGKKYDLEKP
                     FFVLATQNPIEQEGTYPLPEAQLDRFMFQLNVDYPETEEEAEIVKLTTSAYLAQLSTV
                     LSGQEILDIQDLVRRVPIADEVIQYAIRLVKASRPQQGEQTGELADFVKRYINWGAGP
                     RASQYLVLGGKARAILNGRYGVSHEDIQSVARPVLRHRILVNFRAEADGVNSERIIEQ
                     LLQQVPR"
BASE COUNT          628 a          701 c          682 g          622 t
ORIGIN      
        1 cgggttgggg tagacagtgg attccctgtt ggggaagtag aaagcatccc ctcgacctcg
       61 aacctgtatg taaaccgagt tgatgttccg ggcaagaatc ttggcgatga tggtgtcgca
      121 tttcgcctgg gtgttcatct cccaataggg aacataaacg ccccgaaact caggagagtc
      181 tgcccagctc gacagacaga tcgctaccag cgccccaaaa atcaggaatc ttctcatcac
      241 atagcctcca tcatggaact caaagacccg ttcggacaaa gttacccgga cggtgtctac
      301 aaggatgacg gacttcttgc ttttccaggt gcgtgtcacc cgacagcctg ccatccaaat
      361 gaaggttatc cttatggggt ggaaaatgtc aactttcttg ttggacgagg gatttggatg
      421 tagttggtgc aaggagatag aaaaccgcgg cgtgaaacag gcaaagaaga ctttgcgggc
      481 aagcgcgttc tatctctgga ttcatgtgtg agttcgacgt ctgggttgtc ttgggactat
      541 caacaaaagg ccaacccctc tgtcctattt tccgcagcag agtttgaact tcttgccgct
      601 gccgcaggga cacggatcgt ttctcccaac gggcgtgtgg gtctgcattg tcctctgtct
      661 gcgcatctcc agcacctcat cgccaagttt gcgaaaggca tctgcggtat gtgcgaagaa
      721 gcgctggtat gccgagcagt agacgcttcg cccgtctttt cccaagagcc gatccttctg
      781 gcaaccaccg tgacagtgtt ggacaaaacg acagccctga cacgcttcag agactttctt
      841 gtttcgggcg aattcaactc tgcgctccga ctgcgccgcc tcgacgagag tccctgttgt
      901 cagcgaaccc agtttccatt cgggatagac aaagaaatca caggggtaga tgtctccgtt
      961 gtattccacc aagagatagt catcgcatcg gtctccaaac gtacaccagg tcaacatctt
     1021 gcgatccacc gcatagccaa tcatggcgtc aaagagtctg atcgaaattc gaccgacatc
     1081 tttcgggtac cattcatcaa acagatcgca gagaaactgc ccatagatct ccggtgtgag
     1141 aaaccaatcg gttggtttgc cggtctcggg atcaaggtca cagcaaggga tgaactgcat
     1201 gaagttgaac tcattgctca caaaccatcg gtagatgtcc ttggcacggc cttcaacggc
     1261 gggcgtcaag acggtgagtg cattgaactc aacaccatgc cgacgcatga gacgcgcgct
     1321 gttcatgacc tgctcgtgcg tgccttcatc accgaaattc ttccgatgca gattgtgcag
     1381 atcgggggga ccatcaatgc tcaagccgat taagaacttg tactcagcaa ggaaagcgca
     1441 ccatttctcg tccaaaagaa tgccgtttgt ctgcagcgag tttccgacca cctgtcccgg
     1501 ggcaccaaaa cgcttctgga actcaaccac tttcttgaaa aagtccagac ctgcaagcgt
     1561 cggttctcct ccctgccagc catagaccga ttgcttcaga cgaaaagaca tgtgctcacg
     1621 gagcagtttt tcgagaatgg catccggcat tcgctccgtc ggcttctttg gatagatatg
     1681 ttccacacgg cgatagaaac agtattcgca attcaggttg cagcctgcgg agactggttt
     1741 gatgagcaac tgtataggtt tcaatacttc tttaactgac ctcaccgagg gacttgttgc
     1801 agcagttgtt caatgatccg ctcggagttg actccatcgg cttcggctcg gaaattgaca
     1861 agaatgcgat gccgaagcac cggacgggca acggattgaa tgtcctcatg ggaaactcca
     1921 tatcgtccgt tgagaatggc tcgcgcctta cccccaagaa caaggtactg cgacgctcgc
     1981 gggcccgccc cccagttgat atagcgcttg acaaaatcag ccagttcacc ggtttgctcg
     2041 ccctgctgtg gacggctcgc cttgaccaga cgaatggcgt actggatcac ttcgtcggca
     2101 atgggaactc ttcgaacgag gtcttgaatg tccaggattt cctgaccgga aagcacggta
     2161 ctgagttgcg caagataggc agatgttgtc agtttgacga tctccgcctc ttcttcggtt
     2221 tcaggatagt caacattcaa ctgaaacatg aaacgatcga gttgtgcttc gggcagggga
     2281 taggtgcctt cctgttcaat gggattctga gtcgccaaga caaagaaagg tttctccagg
     2341 tcatacttct tcccagaagc agtgacgctg tattcctgca tggcttccag caaagccgcc
     2401 tgagttttgg gcggcgttcg gttgatctcg tccgccagga tgatctgggc gaacaggggc
     2461 cctttcacaa aacgaaaata gcgttcgcct gtcgctgtat ccacttccag gatgtcggtt
     2521 cccgtgatgt ctgaaggcat caagtcggga gtgaactgaa ttcgtttgaa cgtcagagcc
     2581 agcactctgg caatggcttg aaccaacgaa gtcttcgcca gacctggaac acc
//
LOCUS       DAOQUC010000472         2633 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_46772_length_2633_cov_2.584174, whole genome shotgun sequence.
ACCESSION   DAOQUC010000472 DAOQUC010000000
VERSION     DAOQUC010000472.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2633
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46772_length_2633_cov_2.584174"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(156..2531)
                     /locus_tag="PKH07_08930"
     CDS             complement(156..2531)
                     /locus_tag="PKH07_08930"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNT35107.1"
                     /translation="MAVQRIGLGLFLFLFCLVPLMSAQESDMASRCDINRDGTVDEKD
                     LLLVMSFWKHRVGATTTPTWVFTPTDSPTSEPSVSPTETPSPETTETETPTMAETPSS
                     TPTQEITPTETDTPTVQPTDTPTEAPSASPTETSTDTPTIAPTFSDTATSTPSETPTD
                     TPTLTSTETPTATDTPTPTATDTDTPAPPTPTSPIELVLIPGGVSWMGSPQEGTDPDD
                     EKPLHQVYLDTFEIGRYEITRAQYAAFLNAGGYTDNRYWYPKAREWRENDRPDGKPIY
                     WDSPPSWIHNPDWPEDGPAVYLTVHEASAYCSWLAENTGGVYKYRLPTEAEWEKAARW
                     DPSTQTLRVYPWGNLWEPGCCNWNADTLSGGYLDNIMPVGSYPCGASAYGLLDMAGNA
                     AELVIEAYDHDFYRRGHGWDFLEPSIVRYNPLNWVARHAYTGRDNRVGQHTVVRGGDL
                     NADQQKLRAARRHHEWHGLAEINGFRVVRCIVPTPTCTPPPHPTPGEMALIPAGVFVM
                     GTSPTRPHDYGYAAPELPEHEVYLDAYEVGKYEVTRGEFREFIEDQGYDKNQYWPTPA
                     LTPKWALSWHEPGYWTTPPPDWSGGDYPPADNHPVLGVSWYEAQAFAKWKSEKTGDKY
                     RLPTEAEWERAARWNEIEQWGFEYPWGDFFDSCKANTPADPKYAGIQTAPIGSFPEGI
                     SPAGCHDMSGNVWEWTGDYFDTTYYSRGPCGSVPMCGTIWDNPKGASPTPHPTNGCLG
                     RASIRGGSFDKDDGIHGMRTATRGLDGGPCFQSHNYGFRLVREIPTSTPTP"
BASE COUNT          534 a          735 c          734 g          630 t
ORIGIN      
        1 atcgtttcga gcaacgcatc gaagcaggac tgcagcagac gcggacaagt cgcgggaaat
       61 atcactatag tcggagcgtc gtcgaatggc ggtcggcaag ttgttggaca agaacagtta
      121 tgttctttcc atctcgggcg tcatgcagag ccgatctacg gcgtcggtgt cgaggtcgga
      181 atttccctca ccagacgaaa accgtagttg tggctctgga aacaggggcc tccgtccaaa
      241 cctctcgttg ccgttctcat tccatggata ccgtcgtctt tgtcaaagct gcctccacgg
      301 atggaagcac gccccagaca tccgttggtt gggtgaggtg tcggtgaggc tcccttgggg
      361 ttgtcccaga tcgttccgca catcggaacg gatccgcaag gaccccgact gtaataggtt
      421 gtgtcgaaat agtcccccgt ccactcccag acattgcccg acatatcatg gcatcccgca
      481 ggactgatcc cctctgggaa actcccgatg ggtgccgtct gtattcccgc gtacttagga
      541 tctgctggcg tgtttgcctt gcaggagtca aagaagtctc cccagggata ttcaaatccc
      601 cattgttcta tttcattcca tcgcgctgcc cgctcccatt cggcctccgt gggaagacgg
      661 tacttgtctc ccgtcttctc gctcttccat ttggcgaatg cctgcgcttc gtaccaggaa
      721 acacccaaga caggatggtt gtccgcagga ggataatccc cacccgacca gtccggtgga
      781 ggggttgtcc aatatcccgg ctcatgccag gacagcgccc atttcggcgt cagcgcggga
      841 gtcggccagt actgattctt gtcatagcct tggtcttcaa tgaactcgcg gaactcgcct
      901 cgtgtcacct catatttccc gacctcgtag gcgtcaagat agacctcgtg ttcaggaagc
      961 tcaggcgctg cgtagccgta gtcatgagga cgagtcggtg aggttcccat cacgaaaacg
     1021 cctgccggaa tgagcgccat ctctccaggg gtcggatggg gtggcggcgt acatgttggc
     1081 gtcggaacaa tgcaacgaac cactcgaaat ccattgatct ctgccagacc gtgccattca
     1141 tggtgccttc tggccgcacg aagtttctgc tgatcggcgt ttagatcacc gccacgaaca
     1201 acagtgtgct gtccaactcg gttatccctg ccggtatagg cgtgacgcgc cacccaattg
     1261 agaggattgt atcgaacgat gctgggctcc aagaagtccc agccgtgtcc acgtctgtag
     1321 aaatcatggt cataggcttc aatgaccaac tccgccgcgt tgcccgccat gtcaagaagt
     1381 ccatacgcac tcgctccaca tggataactg cctactggca tgatgttgtc caagtatcca
     1441 ccggagaggg tgtccgcgtt ccagttgcag caccccggct cccagagatt gccccaaggg
     1501 tatactcgaa gagtctgcgt ggaaggatcc cagcgcgctg ccttctccca ttccgcctcg
     1561 gtgggtagtc tgtatttgta gacacccccc gtgttctcgg ccaaccatga gcagtaggca
     1621 ctcgcctcat gtaccgtaag gtagacggca ggtccatcct caggccagtc cggattatga
     1681 atccagctgg gcggactatc ccagtatatc ggctttccgt ccggcctgtc attttcacgc
     1741 cactcccggg ctttgggata ccagtatctg ttatcggtat atcccccggc attgaggaac
     1801 gccgcgtact gagctcgggt gatctcatat ctgccaatct cgaaagtgtc cagatacacc
     1861 tgatgaagcg gcttttcgtc gtcggggtct gttccttcct ggggactccc catccaagag
     1921 acaccaccag gaatgagaac caactcgatg ggacttgttg gcgtcggggg cgcaggtgtg
     1981 tcggtgtcag tcgctgtggg tgtcggggtg tccgttgctg tcggtgtctc agttgacgtc
     2041 agcgtcggag tatccgttgg tgtctccgac ggagtagatg tggctgtgtc tgagaaagtc
     2101 ggcgcgattg tcggtgtatc tgttgaagtc tcagtcggcg atgccgaagg tgcttctgtt
     2161 ggtgtatcgg tcggctgcac ggtcggcgta tcggtttcgg ttggagttat ctcctgggtt
     2221 ggtgtactgc tcggagtctc tgccatggtt ggcgtctccg tttcggttgt ctctggcgat
     2281 ggggtctccg tcggggatac agaaggctcc gatgtagggc tgtcggttgg agtgaagacc
     2341 catgtcggag ttgtcgttgc tccaactcgg tgcttccaga aagacatcac gagcaggaga
     2401 tctttttcgt ctacagtacc atcgcgattg atgtcgcatc gggatgccat atccgattcc
     2461 tgtgcactca tcaaaggcac aagacagaag aggaagagaa acaaccccaa accaatacgt
     2521 tgtactgcca ttctcctgaa acctccagat ctgaagaaaa acctcacacc gaggatctga
     2581 gacagatgca tattacagga aatgtgacaa cctgttcaaa ctattctgag cag
//
LOCUS       DAOQUC010000473         2632 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_46790_length_2632_cov_2.848661, whole genome shotgun sequence.
ACCESSION   DAOQUC010000473 DAOQUC010000000
VERSION     DAOQUC010000473.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2632
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46790_length_2632_cov_2.848661"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            311..2356
                     /locus_tag="PKH07_08935"
     CDS             311..2356
                     /locus_tag="PKH07_08935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35108.1"
                     /translation="MKYAEMSLLLGLVLCAPMALADDWISAEVNVETENRIGSIEPDM
                     LGVGLHIGTKQMIGNPESAEQVVQMGFKMIRFPNGCQADEYNWLAAQDKGQVTVDDFL
                     EFCEKTNTTPYYTLNMQGGTDGLDHPIPENASLDERIRYKHHAPNPCGYTAERYHYGT
                     LDEAVQLVSKYTVERFLNGDQPIQHYELGNENWGQCSSDWQPEVYCRTAEVYASAIRS
                     VVNEEAREHPELHSVKLRITAVGYPIMGNNQDPEQATNHKINVAWTEGLNDLYRRGII
                     DAVQEHFYPHSTGTGDALVWTEHNLNNIMLTRRGIPNPRLNGYFDSDLDYEMPIDYTE
                     WNLKCWGPAPQFHPVANADFEEGLANWITEASPERGARLLVTPRARRRGEKGLEIRTG
                     WRTAVAEASITFMPDGKQPDLYMGGWVRTNKPDAVSLILRNAGQVIQERKALSKDRWQ
                     RYLVGADLPEATSEAEFVIRVHGSRVKVWFDNLEAITYASKASRAPVSVNTFEQQLYG
                     VDAIRKMIEHGTALTHFHHLFGNYGCPTLKVDCTPRDNANIFKFYSGRIGTHRLKSET
                     HVGTFDYASHAASWATDFNALAPNTTSIPCLSALATRDDNELHLLLINRSTDRNVRTR
                     IRLSEKPSSETASVRRITSEDYNASGAVLSEHERSVSKTFTHIVPPHTADMITIAME"
BASE COUNT          660 a          713 c          714 g          545 t
ORIGIN      
        1 ataaggcagc agccctcaga accgcttcgc gaacctgaag ggctggcacc cagaggagtc
       61 agtcggagca tgagaagaga gagggcaccc ggcagaatcc ccctttgatc cccccttgtt
      121 aagggaggca ggaccgttct taccctttgg taagggcagg ggggattttc gggagagcat
      181 acctccaccc catccgtaac tgagccagtt ctctctgttt ctgtttatcc tttgtcgcag
      241 ggtgctgcca tgcaataatg gtcttgcatc tcttactgag acggaatcgg aagtaactgg
      301 agaggcgcag atgaagtatg cagaaatgtc tttactcctc ggactcgttc tctgtgcgcc
      361 aatggcgctt gccgacgact ggatttccgc agaggtcaat gttgagactg agaaccgaat
      421 cggctccata gagcctgata tgctgggtgt cggccttcat atcggaacca agcagatgat
      481 tgggaacccc gaaagcgcgg agcaggtggt gcagatgggt ttcaagatga tccgcttccc
      541 aaacggctgt caggctgacg agtacaactg gcttgctgct caagacaagg ggcaggtcac
      601 ggtggatgat tttctcgaat tctgtgagaa gaccaacacg acgccatact atacgctgaa
      661 catgcagggg ggaacagacg gactcgatca tccgattccg gagaacgcca gcctcgatga
      721 gcggattcga tacaagcacc acgctccaaa cccatgcgga tatactgcag aacgctatca
      781 ctacggcaca cttgatgaag cagtgcaact ggtaagcaag tacaccgtcg agcgctttct
      841 gaatggcgac cagcccatcc agcactatga actgggcaat gaaaactggg gacagtgctc
      901 gagtgactgg cagccggaag tctactgcag aacggctgaa gtctatgcgt cggcaattcg
      961 atctgtggtc aatgaggagg cacgtgagca tccggagctg cattccgtca aactccgaat
     1021 cacagcggtc ggctatccga tcatgggaaa caaccaggat cccgaacaag ccaccaatca
     1081 caagatcaac gtggcatgga cggaaggtct gaatgacctc tatcgaaggg ggatcattga
     1141 tgcggttcag gaacatttct acccacacag taccggaacc ggcgatgcgc tggtgtggac
     1201 agagcacaat ctgaacaaca tcatgctgac ccgtcgcgga attccaaatc cacggctgaa
     1261 tggttatttc gattccgatt tggattacga aatgcccatt gactacaccg aatggaatct
     1321 caagtgttgg ggacccgcgc cacagtttca tccggttgcc aacgctgact tcgaagaggg
     1381 attggcgaac tggatcacgg aagcctcacc agagcgggga gcgcgactct tggtcacacc
     1441 gcgagcgcgg cgtcgtggag agaagggtct ggaaatccgc accggttggc gcacagccgt
     1501 cgccgaggca tccatcacgt ttatgcctga tggaaagcaa ccggatttgt acatgggtgg
     1561 ctgggttcgc accaacaaac cagatgccgt atcgctgatt cttcggaacg ccggtcaggt
     1621 gattcaggag agaaaggctc tttcgaagga tcgttggcag cggtatcttg tcggtgccga
     1681 tctgccggag gcgacttcgg aagccgagtt tgtgattcgc gtccacgggt caagggtgaa
     1741 ggtctggttc gacaaccttg aggcgatcac ctacgcaagc aaagcgtcgc gggcacctgt
     1801 ctcggtcaac acatttgagc agcaactgta cggcgtggac gcgattcgaa agatgatcga
     1861 gcacggcact gctctgacac actttcacca cctctttgga aactatggtt gtccaacgct
     1921 gaaagtggac tgcaccccac gggacaacgc caacatcttc aagttctact caggccgaat
     1981 tggaacgcac cgactcaaat cagaaacaca cgttggcacc tttgactacg caagtcatgc
     2041 agcttcgtgg gcaacggatt tcaatgctct ggcgccaaac acaacatcca ttccctgtct
     2101 ttcggcgctg gccacgagag atgacaacga actgcatctg cttctgatca accgaagcac
     2161 cgatcgaaat gtcagaacac gcattcggct ttccgaaaaa ccgtcttctg agactgcctc
     2221 tgttcgccgc atcacttccg aagactacaa tgcttctgga gccgttctct cagaacacga
     2281 gcgatcagtc tcaaagacct tcacacacat cgtaccaccc catacggccg atatgataac
     2341 cattgcgatg gagtgaagac gaagcacttg agcaggcacg aacgttcgtc caagcagtcc
     2401 gcgtcttctt tgagcgcaag agcgactcgg aaagttgaac tctacggtct ccgcgctgaa
     2461 gacaactgat gaggcagaat ctgcaccgcg tatctacctt ctcacaaact tcacggcatc
     2521 cgcagcaaca aagccgtccg cgttgttggt gatttcgata tactggttct cgttggcgcg
     2581 accaaacttg tagattccca gggaatgcca gccgacggag tcccgcgagt ag
//
LOCUS       DAOQUC010000474         2631 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_46808_length_2631_cov_4.133929, whole genome shotgun sequence.
ACCESSION   DAOQUC010000474 DAOQUC010000000
VERSION     DAOQUC010000474.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2631)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2631)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2631
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46808_length_2631_cov_4.133929"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..696)
                     /locus_tag="PKH07_08940"
     CDS             complement(<1..696)
                     /locus_tag="PKH07_08940"
                     /inference="COORDINATES: protein motif:HMM:NF019908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NPCBM/NEW2 domain-containing protein"
                     /protein_id="HNT35109.1"
                     /translation="MAQAVEVTKAELSQRDGWVAVKFEGRQSENQLREGLLVIRNYGA
                     VQQNQRDGLPLRIAETTYARGIFTHAVSRIKVLLPSPGKTFSASVGIDTNGSYVGGSS
                     EFCVVVGDEERYRSPVLCRGESALPIGVDLDGAKEFVLAVTDGGDNVNSDQASWADAK
                     VLMENGEEFWLGDALMFTCDDLPLSTTPPFSFNYGGRPSSEFLSQWSVSRDSRKVDEA
                     RTEHTLRYSDPVTG"
     gene            complement(781..1161)
                     /locus_tag="PKH07_08945"
     CDS             complement(781..1161)
                     /locus_tag="PKH07_08945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35110.1"
                     /translation="MKSCLLLLTFWAMQAVAQILFKYGSSNANRWLLFFILGNVFGAS
                     SIWLLMLLYRHMNVNVALALAAGGGFLFCQLAVALVYHSRLSFVQVIGILGIAICMSL
                     VTFGERKQPEPASNRVQMTESDCS"
     gene            complement(1332..2438)
                     /locus_tag="PKH07_08950"
     CDS             complement(1332..2438)
                     /locus_tag="PKH07_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019535929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNT35111.1"
                     /translation="MKKAKIGVIGCGNICGIYFKAGQTFDILEIVACADIIPQRAEEK
                     AREFGIPKALTVKELLNDPEVEIVLNITPPNGHAEIGIAALKAGKHVYNEKPLTIHRK
                     DAKRMMELAKEKGLRIGCAPDTFMGAGLQTCRKVIDDGWIGRPIAATGFFMGGGPEGW
                     HPNPDFFFKVGAGPMFDMGPYYLTAMVSLLGPVKRVTGSAQISFPKRIATCQQQYGKI
                     IKVETPTHIAGVMDFADGVVGTLITSFDVKAGANLPNLEIYGTEGTLSMPDPNTFAGS
                     VRVRRAGQSEWKEVPFSHIYAENSRGIGMADMAYAIRSKRKHRACGEMAYHVLDLMHT
                     FGEASAKGKHINVESTCERPKPLPLGLRTGILDE"
BASE COUNT          618 a          778 c          628 g          607 t
ORIGIN      
        1 acccgtgaca gggtcactgt acctcagtgt gtgctccgtt cgcgcctcgt ccactttcct
       61 gctgtctctg ctgacgctcc attgggacaa gaattcggat gacggtcttc cgccatagtt
      121 gaaagaaaac ggaggtgtcg tgctcagagg caaatcgtca caggtgaaca tgagcgcgtc
      181 tccaagccag aattcctcac cattttccat aagcaccttg gcgtcagccc aactggcctg
      241 gtcgctgttg acgttgtcac cgccgtctgt taccgcaagg acaaactcct tcgcaccatc
      301 cagatcaaca cctatcggca acgcggactc cccacgacac agcacaggcg aacgatagcg
      361 ctcctcatcg ccgactacga cacagaactc cgaactccct ccgacgtagg agccattggt
      421 gtctatgcct acgcttgcgc tgaaagtctt tcctggggag ggaagcagga ctttgatccg
      481 gctgacggcg tgcgtgaaga tgcctcgagc gtaggtcgtc tcggcgatcc tcaagggcag
      541 tccgtcccgc tgattctgct gcacagcacc atagtttctg atgaccagca agccctctcg
      601 aagctgattc tcggattgcc ttccctcgaa tttcacggcg acccaaccat ccctctgaga
      661 caactctgcc tttgtgacct ctacggcttg tgccatgagc gttcctccta tcatcatcaa
      721 caaggcgaca aatagcacac gtgacattgc agtgaatcgt accatagcgg tgacctttcc
      781 ctaactgcag tcgctctctg tcatctgtac tcggttgctt gcaggttccg gctgtttcct
      841 ctccccaaaa gtgaccaacg acatgcatat cgcgatcccc agaatcccga tcacttgaac
      901 aaaggacaat cgtgagtgat agaccaacgc cactgccagt tggcaaaaaa ggaatccacc
      961 acccgctgcc agggcaagcg ccacattgac gttcatgtgt ctgtagagga gcatgagcag
     1021 ccagatgctt gaagcgccga agacatttcc aagaataaag aaaagcagcc acctgtttgc
     1081 gttcgaactc ccgtacttaa acaggatttg tgcgacggct tgcattgccc aaaacgtcaa
     1141 cagaagaaga caacttttca tagtcctgcc tttcccggcc aacaaatggc gctttccttg
     1201 gagtatactg acgcacattg ccagataccc gcaaactcca aagcaatacc tctacgcaag
     1261 tccgcagtcg cccttcgtca actgaagcgg ttcgccatct gtaagcgcaa cccgtaaggc
     1321 gggtataaag actactcgtc cagaattcct gttcgcagtc cgagaggcag aggcttgggt
     1381 cgctcacagg tgctttcgac gttaatgtgt ttgcctttcg cagatgcctc gccgaaggta
     1441 tgcatcaagt ccagaacatg ataggccatc tcaccacagg ctcggtgctt gcgtttggag
     1501 cgaatggcgt acgccatgtc tgccataccg attccacggc tgttctccgc gtagatatgg
     1561 ctgaacggga cttccttcca ctcactctgt cctgcacgac gcactcgaac cgatcctgca
     1621 aaggtattcg ggtcaggcat gctcaaagtg ccttcggtgc cgtagatctc caagttggga
     1681 agattggcgc ccgccttcac atcaaaggac gtgatgagcg ttccaacaac gccatcggca
     1741 aaatccatca ctccagcgat gtgagttgga gtctcgactt tgatgatctt gccatactgc
     1801 tgttgacagg tggcgattct tttcggaaag gaaatctgag ctgagccggt gacacgcttg
     1861 acaggtccca atagggatac cattgccgtg agatagtaag gacccatgtc aaacattgga
     1921 cccgcaccca ccttgaagaa gaaatccgga ttgggatgcc agccttcggg tccgccaccc
     1981 atgaagaaac ctgttgctgc gatggggcga ccgatccaac cgtcatcaat gactttccga
     2041 caggtctgca accccgcacc catgaacgtg tccggcgcac agccaatgcg caaccctttc
     2101 tcctttgcca actccatcat cctcttggcg tccttgcggt gaattgtcag gggcttctca
     2161 ttgtagacat gcttgcccgc cttcagtgcg gcgatgccga tctccgcatg gccgttgggt
     2221 ggggtgatgt tgagtacgat ctcaacttcg ggatcgttga gtagttcctt cactgtcagt
     2281 gcctttggga ttccaaactc cctggctttc tcctccgccc gctgcggaat gatgtcagca
     2341 caggcgacaa tctcaagaat gtcgaacgtc tgacccgcct tgaagtagat cccacaaatg
     2401 ttcccgcaac cgatgactcc aatctttgcc ttcttcatgc tgatatgctc catgctgata
     2461 gaatgaacca gtctcgtctg aattgatgag gcatcatctc agacaatgca aactgcgaaa
     2521 cgatagcact cagagggcaa caggagcaag cacgactgca tccctatctt ctcacaactg
     2581 tcaacgcaaa gtagaaatgt ccctttttct gcaaaataga aatgtcccca t
//
LOCUS       DAOQUC010000475         2631 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_46811_length_2631_cov_3.746894, whole genome shotgun sequence.
ACCESSION   DAOQUC010000475 DAOQUC010000000
VERSION     DAOQUC010000475.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2631)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2631)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2631
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46811_length_2631_cov_3.746894"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1086
                     /locus_tag="PKH07_08955"
     CDS             <1..1086
                     /locus_tag="PKH07_08955"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNT35112.1"
                     /translation="EAKADLEQAQQAAQRTKNGARQAQVEMAGALLALSVGASREAGE
                     HLNKAGMLLEPVDEPRLKMELANLQGIHAWRAGRLDDAEGLYHGVIEIGDTLSDLTYV
                     ANAYNNLGLIAYSKGDLHRAFELHSKALKVRQQMDDLWGQAASHNNIGIVFENNNHED
                     SAEREYLQALNLARTTGFREVETAALANLGQCAENKGLFPQSLEYNSRSLDLATRLGD
                     RRSQAIALDNLGNTHYLCGLFSEAQDKHGAALRLAESLDDGDICCRALLGLCFDLING
                     GKTDEAESLCDRAEQIVVRLGFNETRPRLYRARAELLLARGDIPGAQDEASKAMRSAQ
                     EMHLRAEETRATVLWNRLRQDKSGNGK"
     gene            1097..1672
                     /locus_tag="PKH07_08960"
     CDS             1097..1672
                     /locus_tag="PKH07_08960"
                     /inference="COORDINATES: protein motif:HMM:NF012271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HNT35113.1"
                     /translation="MRIIRKVHLRILGLCFLISVCAVGVGAFLATPDTSNVEKEYDRI
                     TLGGGESFQGRIVCSDSITLVAGGNVQKSSRFEVISVKTQGNNGIATAIGSNSVYLNW
                     ASSPDGVVQTYTVYRSNSKDGTYEVVAVLGGTVLEYTDTGLNPATTYFYKVVALGVDS
                     SSWTVLGPIGVKTKQVSMTPLAADGNWREYR"
     gene            1695..>2631
                     /locus_tag="PKH07_08965"
     CDS             1695..>2631
                     /locus_tag="PKH07_08965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35114.1"
                     /translation="MKRWAMVVGCLLFTCSMVWAQGVTFNYEGRIKIDGNPYTGDGYF
                     KFAIVDVSGDISHWSNDGTSTVGNEPSAAIVSSVTDGVFNVIIGDASIANMSVLIPSM
                     FNAREKVFLRVWFSDTGSAFEQLVPDREITNPALLGIQTSDELIVYVDAAMGDDRFTG
                     YEEDRPKKTIQSAWDSIPSMVRSNVTVRLAQGIYRESVLLAGKTLIGDATISLVGDRS
                     APGNVILTGADSGNETLPVRTHGLTIRSQKGVYVKGIQLQYFSFSGIYFLSGSHAYVW
                     DCKCLNNRIGIYVTRLSILEAKNVEAGNGLGGSQGI"
BASE COUNT          643 a          614 c          768 g          606 t
ORIGIN      
        1 gaggcaaagg ctgacttgga gcaggctcaa caggccgctc agcggacaaa gaacggcgca
       61 cgtcaggctc aggttgagat ggcgggtgct ttgttggctc tgtccgtggg agcgtcgaga
      121 gaagcaggag agcatctcaa caaggccgga atgttactgg agccagtgga tgagcctcgc
      181 ctgaagatgg aactggcgaa tctccaaggc atccatgcct ggagagcagg ccgactggac
      241 gacgcagagg gactctacca cggggtgatc gagatcggag acacgctcag tgatctgacg
      301 tacgttgcca acgcttacaa caatcttgga ttgatcgcct acagtaaggg tgaccttcat
      361 cgggcattcg aactgcattc gaaagccttg aaagtcagac aacagatgga tgatttgtgg
      421 ggtcaggccg cttcacacaa caacatcgga atcgtgttcg agaacaacaa ccatgaagat
      481 tccgccgagc gtgagtactt gcaggcactg aatctggctc gaacaacagg atttcgagaa
      541 gtcgagactg cagcactcgc caatctcggt cagtgtgcag agaacaaagg actctttcct
      601 cagtcactcg aatacaattc acgctctctc gatctggcaa ctcgcctggg ggacagaaga
      661 tctcaggcaa ttgctctgga taatcttggc aatactcact atctctgtgg tttattttca
      721 gaggcacagg acaaacacgg agctgcactg cgcctggcgg agtctcttga tgatggagac
      781 atctgctgcc gagccctgct gggtctctgt ttcgatttga tcaatggggg aaaaaccgac
      841 gaagccgagt ctctttgcga ccgagcggaa cagattgttg ttcggcttgg tttcaatgag
      901 actcggccga gactgtatcg agccagagcg gagctccttc tggctcgcgg cgatatcccc
      961 ggtgcacagg atgaggcgag caaggcaatg agaagcgctc aggagatgca cttgagggca
     1021 gaggaaactc gggctactgt actttggaac aggttgcggc aggacaagag cgggaacgga
     1081 aagtgaggag agacaaatgc gcatcatccg taaggttcat ctgaggattc tcggtctttg
     1141 ctttctcatt tctgtttgtg ctgtcggtgt tggcgcgttc ttggcaactc cagacacttc
     1201 taatgtggaa aaagaatatg accgcatcac gcttgggggt ggggagtctt tccaaggtcg
     1261 aatcgtctgc tctgattcca tcacacttgt tgcgggaggc aatgttcaga aaagcagccg
     1321 gttcgaggtg atctctgtca aaacgcaggg caacaatgga atcgccacag ccatcggctc
     1381 caattcggtg tatctcaact gggcgtcaag ccccgacggc gttgttcaga cctataccgt
     1441 ctaccggagc aattcgaaag atggaaccta tgaggttgtg gcagttctcg gtggaaccgt
     1501 acttgagtac acagacacgg gactcaaccc ggcgacgacc tatttttaca aagttgttgc
     1561 cttgggtgtt gactcaagtt cctggactgt tctgggccct attggagtga aaacaaaaca
     1621 ggtgtccatg acaccgttgg cggctgacgg aaattggaga gagtaccggt gatgtgcagg
     1681 gaaggagagc agaaatgaaa cgatgggcaa tggttgttgg atgtttgttg ttcacctgtt
     1741 cgatggtttg ggcacaaggc gttacgttca actatgaagg ccggatcaag atagatggaa
     1801 acccctacac gggagacggt tactttaagt ttgccattgt ggatgtcagt ggtgacatca
     1861 gccattggtc caacgacggc accagcacag tgggaaatga gccgtctgca gcgatcgttt
     1921 ccagtgtgac ggacggagtc ttcaacgtca tcatcggcga tgcatccatt gccaacatgt
     1981 cggtgctcat tccaagtatg ttcaacgcga gggagaaggt gttcctgcgt gtgtggttca
     2041 gtgatacggg aagcgctttt gagcagcttg ttccggacag agagatcaca aacccagcac
     2101 tcttgggaat acagacttcc gatgaactca tcgtctacgt ggacgcggcc atgggagacg
     2161 atcggttcac tggctatgaa gaagatcgtc cgaagaagac gattcagtct gcgtgggact
     2221 cgattccgtc catggttcgc tccaatgtga cggttcgatt ggctcagggc atctatcgcg
     2281 agtcggtact tctggcggga aagacactta tcggcgatgc aacgatatcg cttgttggag
     2341 atcgttcagc cccaggcaat gtcattctca ccggagcgga ttcaggtaat gagacactgc
     2401 ctgttcgaac gcacggcctt acgatcagga gtcagaaggg cgtttacgtc aaagggattc
     2461 agcttcagta cttcagcttt tcagggatat acttcctttc tggctctcac gcttatgtat
     2521 gggactgtaa gtgcctcaac aacaggatcg gtatctatgt gacgagactc tcgatacttg
     2581 aggcaaaaaa tgtcgaggca ggaaatggac tgggtggttc tcaaggcatc t
//
LOCUS       DAOQUC010000476         2626 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_46956_length_2626_cov_3.369117, whole genome shotgun sequence.
ACCESSION   DAOQUC010000476 DAOQUC010000000
VERSION     DAOQUC010000476.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2626
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46956_length_2626_cov_3.369117"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..613)
                     /locus_tag="PKH07_08970"
     CDS             complement(<1..613)
                     /locus_tag="PKH07_08970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35115.1"
                     /translation="MTHTGRKNRLFIILLGLFLFINLAVVAVHPVGQEGERVWKVALR
                     SLVDAARPATQHKSNRESAGSLDSGRANQRGETSIPEDNTGKTASASGELDIPAVGDS
                     SAIRSVSDSLLPSSPGTSGSSVEKDEDQVALDTESTRSPHTEAEETALAQARETATAL
                     AAATPSPDENTTTPTALSIPTATCTPNINARTLSLATVSVKPGE"
     gene            complement(610..1686)
                     /locus_tag="PKH07_08975"
     CDS             complement(610..1686)
                     /locus_tag="PKH07_08975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35116.1"
                     /translation="MKERCSSFSFIHRTGRFCNLLNRFILVGLTLAFPLGYCFAIERL
                     IQVTNGAAQPGEETAIFLKMNEGQLYAGGNFWIDYNPQALSIVDVQKTALTTDFILTK
                     GFPKPGRLSIAMASTEGLAAAANADLAIIKIQVLAGAPANSLLSMTFVDAYWFDENSV
                     RHTYLGDNGLVFAGNTVPSETPLQLQVGSDEAEAGTTAALSMTLSLAEGASSVRGTLH
                     FDPSLLLNPTLSLAGDLPGWQESHSVSNERLTFALSGTANLAGTGERILAECEFDVSS
                     SAGAGTHIAVELEQPAVANVNDFGFYVEPVHGEILIADPTAQPTATPTPRTNSLADFN
                     TDGQVDERDLMEFVSTWHQEGDTK"
     gene            complement(1787..>2626)
                     /locus_tag="PKH07_08980"
     CDS             complement(1787..>2626)
                     /locus_tag="PKH07_08980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I repeat-containing protein"
                     /protein_id="HNT35117.1"
                     /translation="GLVGIFTWSPVEKAKHYEILFHQINQMEPRRDGSDPLRPPREMF
                     DPPTSALVEDVTYALVIPDATAGAEYVWKVRAIVDEGVDGVEKLLPTRWSFPRSFVIG
                     PVVRPEEIGEPIVLLSPADGVIFAGPEAQISFSWTQRDPGRLCQVTITDVSGNLVMRQ
                     YRKQRWDAEIVGFGEYKWQVRGVAIGRDGRVLRLGPPSEERGFEIRPDIVVDGAQQLG
                     DLVQDNCVDSGDAIRALRHSTDPENNPLRVRDILAGDCNGDGRVDSGDAIYMLRKAVG
                     SNR"
BASE COUNT          573 a          756 c          700 g          597 t
ORIGIN      
        1 gttctcccgg cttcacgctc accgttgcca gactcagagt ccgcgcattg atgttgggtg
       61 tacaagtcgc ggtggggatg ctcaaagcgg ttggggttgt tgtgttctcg tccggagatg
      121 gggttgcagc agccaacgcc gttgctgttt cccttgcttg tgcaagcgct gtctcttctg
      181 cctctgtgtg aggagatcgg gtactttctg tgtcaagtgc cacctgatct tcgtctttct
      241 caactgaaga gccgcttgtt ccaggcgagc ttgggagtaa cgaatccgag acactgcgaa
      301 tggcgctgga atcaccaaca gcagggatgt cgagctcacc cgaagcggac gctgtctttc
      361 ccgtgttgtc ctccggtatc gaagtctccc cgcgttggtt cgctctgccc gaatccagac
      421 tgccagcact ctcccggttc gacttgtgct gtgttgccgg ccgcgcagca tcaacaaggg
      481 aacgaagcgc aaccttccac acccgctcac cttcctggcc tacaggatgc accgctacta
      541 cagcaaggtt gatgaacaga aacagaccca gcagtatgat gaacagccta ttctttcgcc
      601 cggtatgcgt catttggtgt ctccctcctg gtgccaggtg gagacgaact ccatcagatc
      661 ccgctcgtca acctgtccgt ccgtgttgaa atcagccaat gagtttgtcc ggggtgtggg
      721 cgttgccgtg ggctgtgcag tcggatcggc gatcagaatc tcgccgtgaa cgggctcgac
      781 gtagaaacca aagtcgttca catttgccac agcaggctgc tcaagttcaa cggcaatgtg
      841 ggtgcccgcg cctgcgctgc tggatacgtc gaactcgcat tcagcaagga ttcgttcgcc
      901 cgtgcctgcc agattggctg ttcctgaaag cgcaaaagtc agccgttcgt ttgaaacgct
      961 gtgggactct tgccaaccgg gaagatcccc tgccagagag agcgtgggat tgagcaacag
     1021 gctcgggtca aaatgcagtg tgcctcgtac cgaactggct ccttctgcaa gcgatagggt
     1081 catagacaac gctgctgtgg tacccgcttc ggcttcatca gaacccacct gcaactgtag
     1141 cggtgtctct gagggaactg tgttgccagc aaagaccaag ccgttgtctc ccagataggt
     1201 atggcgcacg ctgttctcat caaaccaata ggcatctacg aaagtcatcg aaaggagaga
     1261 attggccgga gcgccagcca acacctgtat cttgatgatg gcaagatcgg catttgcggc
     1321 agctgcaagt ccttcggtgg atgccatggc aatgctaagt cttcccggct tggggaagcc
     1381 tttggtcaga atgaagtctg tggtgagtgc cgtcttttga acatcaacaa tggagagtgc
     1441 ctggggattg taatcaatcc agaaattccc acccgcgtac agttgtccct cgttcatctt
     1501 gagaaagatc gcggtttcct caccgggttg agctgctcca ttggtcactt ggatgagtct
     1561 ttcgatggca aaacaataac ccaatggaaa cgcaagtgta agacctacta aaataaaccg
     1621 gttcagcagg ttgcagaacc tgcctgttcg atggataaat gagaacgaac tgcaccgttc
     1681 cttcacgcta taggactctc caacctatag tatagccaaa aacaacgcag aggggaaacc
     1741 tttttcaggt ctcccctccg cagtttccgt gtcggcacga ccgcatctag cggttcgagc
     1801 caactgcctt tcgaagcata tagatggcgt cgccagaatc cacacggcca tcgccgttgc
     1861 agtctccagc caagatatcc ctgacgcgaa ggggattgtt ttccggatcc gtgctgtgtc
     1921 tcagggcacg gattgcgtca cccgagtcca cacagttgtc ctgaaccaag tctcccagtt
     1981 gctgggcacc gtccacgaca atgtccggtc ggatctcaaa gcctcgttcc tcgctggggg
     2041 gacccagcct cagcacacga ccatctcgac cgatagccac accgcgcacc tgccacttgt
     2101 attcaccaaa accgacaatt tcggcatccc atcgttgctt tcggtactga cgcatgacga
     2161 ggttgccgct gacatcggta atcgtgacct ggcaaagccg ccctgggtct ctctgtgtcc
     2221 aactgaaaga gatttgggct tccggcccag cgaagatcac accatcggca ggagaaagaa
     2281 gtacgatagg ctctcctatc tcctcaggtc gaactaccgg accaatgacg aacgagcgtg
     2341 ggaaagacca tcgtgtcggg agcagtttct cgacaccgtc cactccctca tcaacgatgg
     2401 ctcgcacttt ccatacatac tccgctcccg cagtggcatc tggtatgacc aacgcatagg
     2461 tcacatcttc aacgagagcg ctcgtcgggg gatcaaacat ctcccgcgga ggccgcagcg
     2521 ggtccgatcc atcccgtcgg ggttccatct gattgatttg atggaacagg atttcatagt
     2581 gctttgcctt ctcgacgggg ctccaagtaa agatgccgac cagacc
//
LOCUS       DAOQUC010000477         2623 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_47047_length_2623_cov_4.228972, whole genome shotgun sequence.
ACCESSION   DAOQUC010000477 DAOQUC010000000
VERSION     DAOQUC010000477.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2623
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47047_length_2623_cov_4.228972"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="PKH07_08985"
     CDS             <1..240
                     /locus_tag="PKH07_08985"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNT35118.1"
                     /translation="ELLAEWSPQNLVASRHPNLLAQAVTRVLTSPDQAREMAQRGLEW
                     LQSRYRWDAVMPRVEQLIESVAAGKGIPPEIRFEE"
     gene            complement(246..328)
                     /locus_tag="PKH07_08990"
     tRNA            complement(246..328)
                     /locus_tag="PKH07_08990"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(293..295),aa:Leu,seq:taa)
     gene            complement(461..1486)
                     /locus_tag="PKH07_08995"
     CDS             complement(461..1486)
                     /locus_tag="PKH07_08995"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNT35119.1"
                     /translation="MTKTRFGIIGVGGWGEFHVRVLQDHPFAQVTAVCDANIGRARSV
                     AEKYGIPFATEDFHQLLKQDDVDSVSIITPDFAHLEPTLAALESGKNVLVEKPMATTL
                     EDCIRISDLASRSKGKFMVDFHNRWSPPFYKAKDAIDKGELGKVQMISYRLNDTIFVP
                     TKMLSWSGKSTVMWFIGSHSLDTLFWLFGKPLQRVYSVARSEVLASKGITTPDYYCCI
                     LEFEGGGTAMLENGWILSESTPNIIDLKCSILGSEGTIYIDGSHHRMLQKYTATEATY
                     PDVFVLPTVYGKPGGFAAESIRHFADCIVENKQPMVGAKEGLAVTKAILAIEESVRSG
                     HPVTLSK"
     gene            complement(1603..>2623)
                     /locus_tag="PKH07_09000"
     CDS             complement(1603..>2623)
                     /locus_tag="PKH07_09000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35120.1"
                     /translation="RKENCQKRDNLKAKQRELTQQISETQTEMETLNKKVAAVLLEAK
                     VETPEQFNEVCAEKDRLVHIEQQIENLEQQRQLLVGETSEAGLAEEERQIVSRLLVLD
                     AKPAPCAPDPKALQTVGQDLSRQKERIQSLRAEVAHEEGQQKAFSDSVRSLADVDEQL
                     ELANADLNRLRDYQDAVLLAMKVIDESARDFHERVFAPEMGRLCADLVSKVTAGRYKE
                     IQLEKELTAIHVIGSNDESLDGSWLSRGTIEQVYFALRVAIGTFLTQGRERIPLVLDD
                     TFANTDDQRLDLIAQLLLRLSEQTQVILFTCHPFQYDRILQTTAETPQQWKTNQLGDF
                     TVSWL"
BASE COUNT          592 a          731 c          650 g          650 t
ORIGIN      
        1 gagctgcttg cggagtggtc gccgcagaat ctcgttgcgt cgaggcatcc caatctgctt
       61 gcccaggcgg tcacaagagt tctgaccagt ccagatcagg caagggagat ggctcagaga
      121 ggtctcgaat ggcttcagtc aaggtaccgt tgggatgcgg tcatgccaag agttgaacaa
      181 ctcatcgaga gcgttgcagc gggcaagggg atcccgccgg agatacgatt cgaggagtga
      241 ggaaataggc gaggtgggat tcgaacccac aagcggtttc ccgcgaggga ttttaagtcc
      301 ctcgtgtata ccgttccacc actcgccctc aaagcgactg acttcggact gaacatagga
      361 tagtttcagc cctgaataag tcaagcgaaa cggtgctgtc actgggggta cagtcctgcc
      421 cttgcttcgt ttgtgtcaac ccaaagacct gatgttcagc tcacttgctc aaagtcaccg
      481 gatgcccgga tcgaacagac tcttcgatgg caagaatcgc ctttgtgacc gcaagccctt
      541 ccttggcgcc caccatcggt tgtttgtttt cgacaatgca gtccgcgaaa tgacggatgc
      601 tctccgccgc aaagccgcca ggctttccat agacagtagg caagacaaaa acgtctggat
      661 atgtggcttc ggtcgccgtg tatttctgaa gcatccggtg gtgactgcca tcaatgtaga
      721 tagtgccttc ggagccaaga atcgagcatt tgagatcgat aatgtttgga gtgctctccg
      781 agagaatcca accgttttcc agcatggcgg ttcctccccc ttcaaactcc aaaatgcagc
      841 agtaatagtc cggcgttgta attcctttgc tggcaagcac ttcagaacgc gctacggaat
      901 aaacgcgctg caatggcttg ccaaacagcc agaacagtgt atccagactg tgacttccga
      961 tgaaccacat cacggtggat tttcccgacc aggagagcat cttcgtcggg acgaaaatcg
     1021 tgtcattgag tcggtaggaa atcatctgca ctttgcccag ctcgcccttg tctattgcat
     1081 cctttgcctt gtagaacggt ggactccatc ggttgtggaa atccaccatg aacttgcctt
     1141 ttgaccgaga cgccaagtcc gagattcgga tacagtcttc cagcgtcgtt gccatcggtt
     1201 tttccaccag cacgttcttg cccgactcca acgcggccag cgtcggctcg agatgcgcaa
     1261 agtccggagt gatgatgctg acgctgtcca cgtcgtcctg cttcaacagt tggtgaaagt
     1321 cctctgtcgc gaatggaata ccgtactttt ctgccaccga acgggctcgc ccgatattgg
     1381 catcgcagac tgctgtcacc tgcgcgaaag ggtgatcctg cagcactcga acgtggaatt
     1441 ctccccatcc accaacgccg ataattccaa atcgtgtctt ggtcatctca taactcctct
     1501 attccatctt gtcatcaggt caagttcctg gatccccaga tcgctgagtg cagcgtatga
     1561 ggttgtgtct tctatcaggg cacagagaat ccgcgcatag ccctagagcc aactgaccgt
     1621 gaaatcaccc aactggtttg tcttccattg ttgaggcgtc tctgctgtcg tttgaagaat
     1681 tctatcatac tgaaatgggt ggcaagtaaa aagaatcacc tgcgtctgtt cgctcaaacg
     1741 aagaagcagc tgggcaatca gatcaaggcg ctgatcgtcc gtgttagcaa acgtatcgtc
     1801 aaggaccagg gggattcgct ctctgccctg cgtgagaaag gttccaatgg cgacacgaag
     1861 agcaaaataa acctgttcga tagtacctcg ggacaaccag gaaccatcca gagactcatc
     1921 gttagatcca ataacatgta tcgcagtcag ttctttctca agttggattt ccttgtagcg
     1981 tccagcagtg actttggaga ccaaatcggc gcaaagtctc cccatctctg gtgcaaaaac
     2041 ccgctcgtgg aaatcccgtg cactctcatc tataaccttc atcgccaaca ggacagcatc
     2101 ctgatagtcc ctcaaccggt taagatcggc gtttgccaac tccagttgtt cgtcaacgtc
     2161 cgccaaactt cgcacactgt ccgaaaaagc tttttgctga ccttcctcgt gggcaacctc
     2221 tgcgcgaagc gactggattc tctccttctg acgcgaaaga tcctggccga cagtctgaag
     2281 tgctttggga tcgggagcac agggcgcggg tttcgcatcc agaaccaaca ggcggctcac
     2341 gatctgcctt tcttcttccg ccagtcccgc ttcgctggtc tcgccaacaa gcaactgcct
     2401 ctgctgctcc aggttctcaa tctgttgctc aatatgaacc agtctgtctt tctctgcaca
     2461 gacttcattg aactgctcgg gcgtttcgac tttggcttcg agaagaacag cagccacttt
     2521 cttgttcaaa gtctccatct cggtctgtgt ttcactgatt tgctgcgtca gctctcgctg
     2581 ctttgccttg agattgtcgc gcttctggca gttctctttt ctc
//
LOCUS       DAOQUC010000478         2619 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_47169_length_2619_cov_3.134945, whole genome shotgun sequence.
ACCESSION   DAOQUC010000478 DAOQUC010000000
VERSION     DAOQUC010000478.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2619)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2619)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2619
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47169_length_2619_cov_3.134945"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            309..>2619
                     /locus_tag="PKH07_09005"
     CDS             309..>2619
                     /locus_tag="PKH07_09005"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HNT35121.1"
                     /translation="MAKKKNVFKTDFGGAPHATDPEALFRDLRGRSPDIRHLWSHQAD
                     LLREYHRSFKNTKDVAIELPTGAGKTLVGLLVAEFRRQTLGERVAYLCPTRQLARQVG
                     AQATKYGIKAHVFIGKQRDYPAKEYSEFQSSKALAITTYSAIFNSNPRIRDAQSLILD
                     DAHASENYIAGMWSVEITREDSGKLYRAIVDLFREGLPSSFYADITNGIEGNERETSL
                     IELIPGRHVRTYASGLRDLLDNGIEEETPGWYAWEIIKEHTAACGIFVSWNSILIRPF
                     IPPALTHTPFTQANQRVYMSATLGPGGDLERITGVKSIQRLPIPAGWDKRGSGRRLFL
                     MPQVTMSDQEALQVVLSATKDFDRTLVLSPTQYEAAEVRSHLTSIGLTVLGAKDIEDS
                     IDPFVTNDGAALVLSRYDGLDLPDEACRLLVIGGLPSGTNLQEKFLWSRIAASSLLRD
                     RVLTRFTQGVGRCTRSDNDFAVVFIWGRPLVDFVLKQDNRKMLHPELQAELQFGIENC
                     QDKNADDFRELWEAFIEQDEGWESAEEAIVRLRDQYSRHDDPISERLCAAVTDEVEYL
                     YSMWNGDFESALEQARKVADSLQGDETKGYRGWWYYLAADAAMALHEATRDHNLLETA
                     KDCLNRAGKCCPVISWFARLARVTRGNTEQQEVDEVTAIAIENIRSKLTDWGTVGQRF
                     EREVEQAINDLQTTAHKKYHRGLKVLGEMLGFRAELPDGDGDPDCVWSIGNDYYIVHE
                     AKSDHTPSDPIGINDIRQAQSHEDWVRSHR"
BASE COUNT          641 a          674 c          764 g          540 t
ORIGIN      
        1 tactccggca agcgcctctg cgcacttcgt gacagcggtc gggtgacgct tttcccggca
       61 agttatcgac tggtctacga gcatgaccga gcgaaggaga agtcggatga atgacctcaa
      121 cgcttggttc aaagatcgac cgaggtggat gcaggaagcc gccagtcttc tccttgacga
      181 agggccgcat ggcggatggt gattcgaaac gttcctcggc aagtgtctgc gggaggtcgg
      241 cttaaaggac agggcgacgt gaggcactac gaaatgagga aaaacggttg gagcagaggt
      301 caaatgtaat ggctaagaag aagaacgtat tcaagaccga ttttggcggt gcaccacacg
      361 ccactgatcc agaggcgctg tttcgagact tgcgtggccg gtcgccagac attcggcacc
      421 tgtggtcaca tcaagcagac ctattgaggg agtatcaccg gagttttaag aatacgaagg
      481 atgtggcaat cgaacttccc acaggcgcgg gaaagaccct tgttgggctg ctcgttgccg
      541 agttccgccg ccagaccttg ggcgagcgag ttgcctacct ttgtccaaca cgacaacttg
      601 cacggcaggt cggggcgcag gcgacaaaat acggcatcaa agcgcatgtt ttcattggga
      661 agcaaaggga ttacccggcg aaggagtaca gtgaattcca gtcgagtaaa gcgttggcga
      721 taaccacata cagtgccatc ttcaactcca acccgcgcat ccgcgatgca caatctctca
      781 ttctggacga tgcccatgcc agcgagaact acatagctgg tatgtggtcg gtggaaatca
      841 ccagggaaga cagcggaaaa ctttacaggg caatagtgga cctttttcga gaaggtctgc
      901 cttcatcttt ctatgccgac atcaccaacg ggatcgaggg taacgagaga gagacgtcac
      961 tgatcgagct gatcccgggc aggcacgtca ggacttacgc atctggacta agggacctat
     1021 tggacaacgg gattgaggaa gaaacgcccg gctggtatgc ttgggaaatc atcaaggaac
     1081 acactgccgc ctgcggtatc ttcgtttcct ggaattcgat actaatacgc ccgttcattc
     1141 cgcccgcgct tacacacacc ccgttcacgc aggctaacca gcgagtgtat atgtcggcca
     1201 cgctcggccc gggcggtgat cttgagcgga ttacaggcgt caagtctatc cagaggctac
     1261 cgatcccagc gggatgggat aagcgcggta gtggccgtcg actcttctta atgcctcagg
     1321 tgactatgtc agaccaggag gcattgcagg tcgttctctc cgccaccaaa gatttcgata
     1381 ggactctcgt cctcagcccc actcaatacg aagcggcaga ggtccgcagt cacctaacga
     1441 gcatcgggtt gaccgttctg ggtgcaaagg acattgaaga ctccatagac ccattcgtca
     1501 ccaacgatgg tgcagcgctc gtcctttcgc gatatgacgg cttggacctt cccgatgagg
     1561 cgtgtcgact actcgtcatt ggggggttgc ctagcggaac gaatcttcag gagaagttcc
     1621 tttggtcccg gattgccgca tcctctttgt tgagagaccg cgtcttgacg agattcaccc
     1681 aaggggtcgg acgatgcaca cgatctgata atgactttgc tgtggtgttc atttggggac
     1741 gccctctggt cgattttgtt ctgaagcagg acaatagaaa aatgctgcat cccgaactgc
     1801 aggctgaact ccagttcggc attgaaaact gccaggacaa gaatgcagat gatttcaggg
     1861 aattgtggga ggccttcatc gagcaagacg agggttggga atctgcagaa gaggccatcg
     1921 tgcgcttgcg cgatcagtac tcgcgtcatg atgaccctat atccgaacga ctgtgcgccg
     1981 ctgttaccga tgaggtggag tatctttact cgatgtggaa cggagatttc gaatctgcat
     2041 tggagcaggc aaggaaggtc gccgattctc tgcagggtga tgaaacgaaa ggctacagag
     2101 gctggtggta ctacctggca gccgatgcag cgatggcgct tcatgaggca actagggacc
     2161 acaacctgct tgaaaccgcc aaggattgcc tgaatcgggc gggcaagtgt tgcccggtga
     2221 tcagctggtt tgcgcgcctt gcccgagtaa ctcgtggcaa tactgaacaa caggaagttg
     2281 acgaagttac ggcgatagca atcgagaata tacgttccaa gctgaccgat tggggcacgg
     2341 ttgggcaacg atttgaacga gaagtggagc aagcaatcaa tgacctccaa acgacggcgc
     2401 acaagaaata ccaccgaggc ttgaaggtgc tcggagaaat gctcggattc cgcgccgaac
     2461 tacctgacgg cgacggtgac ccggactgcg tctggtccat cggtaacgac tattatatcg
     2521 tccacgaggc caagtcggat catacgccca gtgatcccat cggaatcaac gacatccggc
     2581 aggcccagag ccacgaggac tgggtgcgaa gccaccgtc
//
LOCUS       DAOQUC010000479         2619 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_47183_length_2619_cov_2.320983, whole genome shotgun sequence.
ACCESSION   DAOQUC010000479 DAOQUC010000000
VERSION     DAOQUC010000479.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2619)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2619)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2619
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47183_length_2619_cov_2.320983"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..947)
                     /locus_tag="PKH07_09010"
     CDS             complement(<1..947)
                     /locus_tag="PKH07_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35122.1"
                     /translation="MNSTSTQSQIKAGSLANVSDKAMSLLGKSGTSGKYGESSGDAKR
                     LAQNTVFYLGMGTCGLGAGAGKTLEVLKAELAARGATAHIVEVGCIGLCSAEPMMDVQ
                     LPGRTRVSFMQITAERVPSFLDAVLNGEMPSERVLGQHRCNQCTAWEGVPFIDEHPFF
                     APQTRWVLANCGIIDPNQIEQYGARGGYEALRKALQTMTPSEVCDEVERSGLRGRGGG
                     GFPTGRKWKFALNAESPQKYLICNADEGDPGAFMDRAVIEGDPHRLVEGIAIAAYAIG
                     ASKAYIYIRAEYPLAIGRLKNALAQARECGLISSNILDTG"
     gene            complement(944..1417)
                     /gene="nuoE"
                     /locus_tag="PKH07_09015"
     CDS             complement(944..1417)
                     /gene="nuoE"
                     /locus_tag="PKH07_09015"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HNT35123.1"
                     /translation="MATSIETILEQHPHAGRDSLIPILQEVQENEGFISQDAVVRISD
                     HLGLAASKIYGVSTFYNQFRFHAPGRFHIQVCRGTACHVKGSAAVLESLMRALKLKSG
                     ETTRDGLFSLEVVACIGACGQAPVIAVNGEFHAKVTPDSVVEIIEDYRRQAAERS"
     gene            complement(1445..2164)
                     /locus_tag="PKH07_09020"
     CDS             complement(1445..2164)
                     /locus_tag="PKH07_09020"
                     /inference="COORDINATES: protein motif:HMM:NF003995.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HNT35124.1"
                     /translation="MEYSSPVKVRSPFVQSLYSLRHANSRKIAKSKDPDVSEKTIGRL
                     SQYRRILKELLDQSLGNVYSHELARRAGVSAAQVRRDLMVVGYSGNPHSGYEIVALIK
                     SISNFLDDSQVQRVALIGVGSLGRAILSQFAGNGTRLQITAAFDTSGNASSSVVTGCR
                     CRPIEELEQVVQGEGIKVGLIAVSSTEAQAAADRLIEVGVLSIVNLTPASIQVPDYVH
                     VENLDITMSVERAAFYARQRD"
     gene            complement(2238..>2619)
                     /locus_tag="PKH07_09025"
     CDS             complement(2238..>2619)
                     /locus_tag="PKH07_09025"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNT35125.1"
                     /translation="MRDTKVVLLIDDDVDFLASNRLLFESEGFSVVTCSDGDEGLEKA
                     KTCKPAVIVLDVMMKTSTEGFHLAKQLRSLQETKSVPIVMLTGVNQHAPFKFEAEDTW
                     LPVDKFLEKPVAASQLLAEVKAVI"
BASE COUNT          597 a          765 c          646 g          611 t
ORIGIN      
        1 ccggtatcga gaatgttgga gcttatgaga ccgcattccc gtgcctgcgc cagagcattc
       61 ttgagtcggc cgatcgccag agggtactcc gccctgatgt agatgtaggc tttggaagcc
      121 ccaatggcat aggctgcgat ggcgatccct tccaccaaac gatgcggatc cccctcgatc
      181 actgcccgat ccatgaatgc tccgggatcg ccttcatcgg cattgcagat gagatacttc
      241 tgaggcgact cggcattgag cgcgaatttc cattttctgc ccgttggaaa accaccgccc
      301 ccacgcccgc gaagtccact gcgctccacc tcatcgcaga cctctgaagg agtcatggtt
      361 tgcagagcct tccgtagagc ctcatatccc ccgcgcgctc catattgctc tatttgattt
      421 gggtcaatga ttccgcaatt ggcaagaacc catcgtgtct gaggtgcaaa gaagggatgc
      481 tcgtcaatga atggcactcc ttcccaggcg gtacactgat tacaccgatg ctgcccaaga
      541 actctttccg agggcatctc tccattgagc actgcatcca gaaatgaagg cactcgctcc
      601 gcagtaatct gcatgaatga aacccgagtt ctgcctggca gctgcacgtc catcatcggc
      661 tctgcagaac acagtccgat acaaccgact tcgacgatgt gcgctgtagc ccctcttgca
      721 gcaagttccg ccttgagaac ttccaacgtc ttgcctgcac cggctcccag tccgcaagtt
      781 cccatgccaa gatagaagac agtattctgg gcaagcctct tggcatcacc agaagactca
      841 ccgtacttgc cgcttgtgcc gctcttgccg agcagggaca tcgccttatc agaaacattg
      901 gcaagcgagc cggccttgat ttgactctgt gttgaagttg agttcatgat cgctccgccg
      961 cctgtctgcg gtaatcctct atgatctcta cgacgctgtc aggagtgacc ttggcgtgaa
     1021 actctccgtt tacggcaatg acgggagctt gaccacaagc tcctatgcag gcaacgactt
     1081 ccaagctgaa aaggccatcg cgagtggtct cacccgactt gagctttagt gcccgcatca
     1141 gagattccag aaccgccgcg gagcctttga catgacacgc agtgccacga cacacttgaa
     1201 tatgaaatcg tcccggtgcg tgaaatcgaa actggttgta gaaggtcgaa acgccataga
     1261 tcttgcttgc agccaagcca aggtgatcgc taattcgaac gaccgcatcc tgtgatatga
     1321 atccctcgtt ttcctgcact tcctggagga tcgggataag ggaatcccgt cctgcatggg
     1381 gatgctgctc aagaatggtc tctatggaag ttgccatagt cagtcctctt ctcctgccct
     1441 gtgttcagtc tctttggcgt gcataaaatg ctgcccgctc aacagacatg gtaatgtcca
     1501 gattctcgac atgaacgtag tcgggaacct gaatagaagc gggtgtcaga tttactattg
     1561 agagcacccc aacttcgatc agtcggtcag ccgcggcctg ggcttcagta gatgagacgg
     1621 cgatgaggcc gactttgata ccctcgccct gtacgacctg ttcgagttcc tcgatcggac
     1681 ggcatctgca acctgtcaca acgctgctgg atgcgttgcc ggaggtgtca aaggcagcag
     1741 taatctgaag cctcgttcca ttgccagcaa attgtgaaag gatggctctt cccagactgc
     1801 caacgccaat caaggcgacc cgttgtacct gagaatcgtc caagaagttg ctgatgctct
     1861 tgatgagcgc cacaatctca tatccagaat gcggattacc tgagtacccg acaaccatga
     1921 gatcccgacg cacctgtgca gcagaaaccc ctgcacgccg cgccagctca tgcgagtaca
     1981 cattccccaa ggactgatcg agcagttcct tgagtatcct ccgatactga ctgaggcgac
     2041 cgatcgtctt ctcggacacg tcgggatcct tgctcttggc tatctttcga ctgtttgcgt
     2101 gtctcagact gtagagactc tgtacgaatg gacttcgtac tttgacgggt gatgaatact
     2161 ccatagaata gctcactccc tctaatcgtg ccctcactcc tcattcgcac cggacaaggt
     2221 ggagagcaat agatcgttca gatgaccgcc ttgacctctg cgagcaactg ggaagccgca
     2281 accggcttct ccaggaactt gtcaaccgga agccatgtat cttcagcctc aaacttgaat
     2341 ggggcgtgct ggttgactcc agtcagcatg acaatcggaa cagacttcgt ctcctggagg
     2401 cttcgaagct gcttggcaag gtgaaatcct tcggtggaag tcttcatcat gacgtccaaa
     2461 acaatcaccg ccggcttgca ggtcttggcc ttttcgagac cttcatcccc gtccgaacac
     2521 gtgactacgg agaagccctc gctctcaaac agcagcctgt tcgatgcaag gaaatcaaca
     2581 tcgtcatcga tcagcagaac cacttttgtg tctctcatt
//
LOCUS       DAOQUC010000480         2618 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_47200_length_2618_cov_3.502536, whole genome shotgun sequence.
ACCESSION   DAOQUC010000480 DAOQUC010000000
VERSION     DAOQUC010000480.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2618
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47200_length_2618_cov_3.502536"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(788..2224)
                     /locus_tag="PKH07_09030"
     CDS             complement(788..2224)
                     /locus_tag="PKH07_09030"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNT35126.1"
                     /translation="MEKEAIELSVLIVEDDPNTLLMFGRVVESQGAKCVLVGTFEDAM
                     KATTDQRFDMLLVDRKLPDGDGIDLIEQVRHTDPKALVVMVTAHGTIDIAVQAMKMGA
                     WDFLAKPFCFERLEVLLQRVKEHLASQRENERLRREVMEVRAGEMIGSSPAIRSVKER
                     IAQIAPTPSTVLIQGETGVGKELAARAIHRMSSRSSGPFVVVDCAAIPETLLESALFG
                     HEKGSFTDAHVTRKGMVELAAGGTLLLDEVAELAPSLQSKLLRLLQERVYRPIGSETE
                     KSANIRVVSATRWDLSSRIRSGLFREDLYYRLNVITLNIPPLQKRPSDIPVLANWFLY
                     QQSVRAEKAMEGISEPAMQLLKRYDWPGNVRELQHAIEHAVALAPGTMIEADYLPKTI
                     LESASVNSLPRSFTHAPKAFVQPDLSESMSWKDYQEKAMEPHLKSYVQSLLQQHGGNV
                     SAAAKHAGLSRNGFYMLMRRANWESDGA"
     gene            2357..>2618
                     /locus_tag="PKH07_09035"
     CDS             2357..>2618
                     /locus_tag="PKH07_09035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35127.1"
                     /translation="MQTCVLLCLILFSLRSTAHAEQQTIRISSFQTVPPICRASRPAA
                     LSATITNAGTEPREATLRLTLPEGVRLLKGEQETNLSLNAVSE"
BASE COUNT          607 a          714 c          634 g          663 t
ORIGIN      
        1 agaggggtgc gttccacgca caacgcaccc ctgagggaaa gaaaggaagg agaaaccttt
       61 gatcggtttc atttgggctt catgttgttc gtttccgatg aacttcatct ttgctcgatt
      121 cgcggagttt ttcgttctct tccgataccc agtctccgct cacttacacc agttcgaatc
      181 ctctatctgc tccggagaac caccgttccc cgtttctctt tcgggcgggt cgggaacccg
      241 acccgctccc cttaaagaag ccacttggct tgtgtgcctt gtaaggtcgc taacgccctg
      301 aacagagtaa gaatcttttt tcctgcacct ttcaagccat cacaacacta ctggcctcaa
      361 accctacgta ttccttttga tcttttagca ctttcgatgc caatttactt ctgtaagcat
      421 gaaactttat tatatattga ttagtcttga cttatgccct aaggccttct gtttctcctt
      481 cttctgggtg tggaatatgc tcactaaagt gagcagactc cgtaggcacg ccctaccggg
      541 taacttgagt cagagaggaa tgaatacttt atcttgtgtc ttcgcagtga gaaatgccca
      601 tatcctgtgt caaaaaggca ggtcgcaaga actgtcatca gaagataaca gcctcatgct
      661 tgggttggtt cgcaggagaa tcaggtatac ttttcggttt gctgggccga atctgttcaa
      721 atatgagaca tattatgcgc tttagtgggt gtcaactgaa atgagcacat gctaagtaaa
      781 ttgagcacta cgcgccatca ctttcccagt ttgcacggcg cataagcatg tagaaaccgt
      841 ttctggagag tcctgcgtgc tttgcagcag cactgacgtt tccaccatgc tgctgcagaa
      901 gtgactggac ataggacttg aggtgtggct ccatagcctt ctcctgatag tccttccaag
      961 acatgctctc ggaaaggtca ggttgaacaa acgctttggg cgcatgggtg aaagacctcg
     1021 gcagcgaatt gacagaggcc gattcgagaa tcgtttttgg cagatagtcc gcctcgatca
     1081 tggttcctgg tgccaaagcc acagcgtgtt cgatggcgtg ctgcagttcc cgcacgtttc
     1141 caggccagtc gtacctcttc aggagttgca tggccggttc ggaaatgccc tccattgctt
     1201 tctcggcgcg aacgctctgt tggtagagaa accagttggc cagcacggga atatcactcg
     1261 gtcttttctg cagtgggggg atattcagcg taatgacgtt gagacggtag tacaagtcct
     1321 cacggaacaa accacttcgg atcctcgaac tcaaatccca ccgggtagcg gagacaacgc
     1381 gaatgtttgc gctcttctcc gtttccgaac cgatggggcg gtagactctt tcctgcaaaa
     1441 gtcgaagtaa cttggactga agactgggag ccagttccgc cacttcgtcc agcagcagcg
     1501 tgccaccggc agccagttcg accatcccct ttcgggtaac atgggcatcg gtaaaagagc
     1561 ctttctcgtg tccaaacaat gcgctttcca gtagggtctc cgggattgcg gcacagtcca
     1621 ccacgacaaa aggtcccgag gaacgggaac tcatccggtg tatggcacgg gctgccaact
     1681 ccttcccaac gccagtctct ccctgaatca agacggttga gggcgttggg gcaatttggg
     1741 caatgcgttc cttgaccgat cggatcgccg ggcttgatcc gatcatctca cctgctcgca
     1801 cttccatgac ctcacggcgc agacgttcat tctcacgctg agaggccaag tgctctttga
     1861 cccgctgcag aagcacttcc aggcgctcaa aacaaaaagg ctttgcaaga aaatcccaag
     1921 cccccatctt catcgcctga acggcaatgt ctatcgtccc gtgcgcagtc accatcacaa
     1981 cgagagcctt gggatcggta tgtcgcacct gttcaatgag atcaatcccg tcaccgtctg
     2041 gcagctttct atccaccaaa agcatgtcga aacgctggtc tgtggtcgcc ttcatcgcgt
     2101 cttcaaacgt tcccacaagg acgcacttgg caccttgtga ttccaccacc cgaccgaaca
     2161 tcaaaagcgt gttgggatca tcttcaacga tcagaacgga gagttcaata gcctcttttt
     2221 ccatcggatt tgcgtgtatg catctgcccc ttcagaaatc gcatttgtct tatcaacaaa
     2281 gacactgtaa tttatcccag tattcaaaaa gtgtaaagag aatccttggg gctggaaagt
     2341 tgaggtcatc gccatcatgc agacttgtgt tttgctctgt ctgattctgt tttcactccg
     2401 aagcacggct catgcagagc agcagaccat tcggatcagc agttttcaaa cagttccgcc
     2461 gatctgtcgt gcgagtcgtc cggcggcact ctctgcgacc attaccaacg cagggacaga
     2521 accccgtgaa gcaacgcttc gtctcaccct tcccgaggga gtgagactgc tcaagggaga
     2581 gcaggagaca aatctctctt tgaacgcagt atctgaac
//
LOCUS       DAOQUC010000481         2612 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_47380_length_2612_cov_3.077826, whole genome shotgun sequence.
ACCESSION   DAOQUC010000481 DAOQUC010000000
VERSION     DAOQUC010000481.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2612
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47380_length_2612_cov_3.077826"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(122..2215)
                     /gene="fusA"
                     /locus_tag="PKH07_09040"
     CDS             complement(122..2215)
                     /gene="fusA"
                     /locus_tag="PKH07_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011365698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNT35128.1"
                     /translation="MGVKTMDVVASLAKLRNIGIMAHIDAGKTTTTERILFYTGKTHK
                     LGDVDDGDTETDWMALEKERGITITSAATTCMWRDHQINIIDTPGHVDFTVEVERSLR
                     VLDGAVAVFCAVGGVEPQSETVWHQADRYRIPRIGFVNKMDRMGADFDRAVQMMKDKL
                     QAPVVVTQFPIGCEEKFQGVVDLVSMQAIYWTETDLGSTPRFDEIPEHLRERAEQMRA
                     ELVEKAVEHDEALLEKYFNGQEIDSDEIRRALRHLTVRNLGVPVLCGAAVRNKGVQPL
                     LDAIVDYLPSPMDIPPVEGWHPETEAVLRRAPSLEEPLCALAFKIMADPHGKLTFVRV
                     YSGKLKSGDQVWNVNKKKKQRAAKLFHIHANKRQAVEELRAGDIACVLGLNETVTGDT
                     LTDRAHAIVLGSMQIPEPVISVAIEPRTMADEEKLNDSLAKLADEDPTFRVHRDSETN
                     QLIISGMGELHLEILVERLLREYAVSANVGKPQVSYRETIRKAAEAEGKLERQFGGKT
                     QFAQVLLRVQPLENGTGFQFESVLREGQIPKDFIAAVKQGVSESMSAGVLAGYPMVDV
                     KITLLDANMDIENSTEIAFEVAASIAFKEASRKADPVMLEPIMKLEIIVPEEYMGEVI
                     GNVNSRRGKLCGMEGHKRLQIIDAEVPLAECFGYATTLRSLTQGRGNYTMQVSRYEQV
                     PSDVASELLIRLRGY"
     gene            complement(2385..>2612)
                     /locus_tag="PKH07_09045"
     CDS             complement(2385..>2612)
                     /locus_tag="PKH07_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018083820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HNT35129.1"
                     /translation="GATYQVPVEVRPNRRTTLAFRWLLSAARSRSERTMALKLASELI
                     DASNNQGTAIKKKLDTHRMAEANKAFAHYRW"
BASE COUNT          572 a          771 c          622 g          647 t
ORIGIN      
        1 gatggtgccc acgtttacat ggggcttcgt tcgctcaaat ttcgccttgg ccatttgcgc
       61 acatcctccc ctgacttagt atttctggct ctcctgtttt tctcgagacc cttgccctga
      121 actagtaacc tctcagccgg atcagaagct cggatgcgac atccgagggc acctgctcgt
      181 agcgagagac ctgcattgtg taatttccac ggccttgtgt caaggatcgc aacgttgtcg
      241 catatccgaa acactctgcc aaaggaactt cggcatcaat gatctgaagt cgcttgtgtc
      301 cttccatgcc gcagagtttt ccgcgtctgg aattcacatt tccaatgacc tcgcccatat
      361 actcttcggg aacgataatt tcaagcttca tgatcggctc caacattact ggatcagcct
      421 ttcttgaagc ctctttgaac gcaatggaag cggctacctc aaacgcgatc tctgtcgagt
      481 tctcgatgtc catatttgca tccagcagcg ttattttgac atccaccatg gggtacccgg
      541 caagaactcc cgccgacatg gattcggaga ctccctgttt cactgctgcg atgaagtcct
      601 tgggaatctg tccttcccga agtacactct cgaactgaaa tcccgtgcca ttctccaagg
      661 gttggactcg aagcagcact tgggcaaact gggtcttgcc accgaactgc ctctcgagtt
      721 tgccttccgc ctctgcggct tttcgaatcg tctcccggta tgaaacctgg ggtttgccca
      781 cattcgccga gacggcgtat tccctcagga gtctctcgac aagtatctcc agatgcagtt
      841 cacccatgcc ggagataatc agctgattgg tctcggagtc gcggtgaact ctgaaagttg
      901 gatcttcgtc ggcgagtttc gccaggctat cattgagttt ttcttcatca gccatcgttc
      961 ggggttcgat ggcaaccgag atgactggct ctggaatctg catggagccc aagacaatgg
     1021 catgtgccct atccgtaagg gtgtccccgg tcaccgtctc attgagcccc agcacacagg
     1081 caatgtcgcc ggcacggagt tcttccactg cctgccgttt gttggcatga atgtgaaaga
     1141 gcttggccgc acgttgtttc ttcttcttat tgacattcca gacttggtcg cccgacttga
     1201 gtttgcctga atagacgcgg acgaaggtca gtttaccgtg cgggtcggcc attattttga
     1261 aggccagcgc acacagtggc tcttccaaag aaggtgcacg cctcaaaaca gcttccgtct
     1321 ccggatgcca tccttcgacg ggcggaatat ccatgggaga gggaagataa tctacaatgg
     1381 catcgagaag aggctggacg cccttgttgc gcaccgcagc accacaaaga acgggaacgc
     1441 ccaaattgcg aacggtcaga tgccggagtg cccttcggat ctcatcggaa tctatctcct
     1501 gaccgttgaa gtacttttcc agcagcgcct catcgtgctc gacggccttt tcgaccaact
     1561 cagcccgcat ctgctcagct cgttcccgca agtgctcggg tatttcatcg aaacgcggag
     1621 tgctgcccaa atctgtttca gtccagtaga tcgcctgcat tgagacaaga tcgacgacgc
     1681 cctgaaactt ttcctcgcaa ccgattggga actgagtcac caccacagga gcctgcaact
     1741 tgtccttcat catctggaca gccctgtcaa agtctgctcc catgcgatcc attttgttga
     1801 caaaaccaat acggggaatt cgataacgat ccgcctgatg ccagaccgtt tctgactggg
     1861 gttccacacc accaacagca cagaagactg cgactgcgcc atccagaacc ctcagacttc
     1921 gctcgacttc caccgtgaaa tccacatgcc cgggtgtgtc aataatgttg atctgatggt
     1981 cgcgccacat acaggtggtc gcagcagagg tgattgtaat cccccgctcc ttctcaaggg
     2041 ccatccagtc ggtttccgta tcaccgtcat caacatcacc cagtttgtgg gtctttccgg
     2101 tgtagaacag aatccgctct gttgttgtgg tcttcccggc gtcaatatgg gccataatcc
     2161 caatatttct gagcttcgcc agggatgcca ccacatccat cgtcttcaca cccatcgtaa
     2221 aaactcgtat ccttctcgac ttccaacttg ctggctcgca acgctgtcga gcccgaaacc
     2281 gccgcatcat acacacaaag aaatccgaag tcttctcttt ggtaacaaat cagacctcgg
     2341 acctcttcag aggctaagag cgtgaaccgt aacagcacca cccactacca acggtagtga
     2401 gcaaaagcct tgttggcttc ggccattcgg tgcgtgtcca gctttttctt gatcgctgtt
     2461 ccctgattgt tggaagcatc gatcaactcg gaagccagtt tcagagccat cgtgcgctct
     2521 gaacgacttc gcgcggctga gagcaaccac cgaaatgcca gcgtcgttct acggttcgga
     2581 cggacttcaa cgggaacctg atacgtggca cc
//
LOCUS       DAOQUC010000482         2609 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_47469_length_2609_cov_3.861002, whole genome shotgun sequence.
ACCESSION   DAOQUC010000482 DAOQUC010000000
VERSION     DAOQUC010000482.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2609
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47469_length_2609_cov_3.861002"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..604)
                     /locus_tag="PKH07_09050"
     CDS             complement(<1..604)
                     /locus_tag="PKH07_09050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35130.1"
                     /translation="MRTEQIATGLLLAVMIASSGFAAQDIPDSWLSQVQNDIAASEYN
                     ITLQSDAAPSTLGGEGRGEETSSCPDSTRGDLLPPSPLLRGDQGGITGFYQAPNRKQN
                     LRTYFTDSGIRVVRRTETNPSWEWGLELVGCTKDEGGRMRDELGNAPIPPVEGRKATG
                     NRFEFNRGNVTEWYVNSPEGLEQGFTINQPQSLKDTKDSDN"
     gene            1084..2082
                     /gene="ilvC"
                     /locus_tag="PKH07_09055"
     CDS             1084..2082
                     /gene="ilvC"
                     /locus_tag="PKH07_09055"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007223015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HNT35131.1"
                     /translation="MARIYYDKDASLDILKGKKIAVIGYGSQGHAQAQNLRDSGLTVI
                     VSELEGTANYDLAVEHGFKPMPAAEAAKQADLIQILVPDQTQAMLYRGAVQQAMGPGK
                     TLLFSHGFNIHFGQIVPPATVDVIMVAPKGPGHLVRRVYTEGGGVPCLIAVQQDASGK
                     AKETALAYARGIGGTRAGVLETTFREETETDLFGEQVVLCGGLTELIRAGFETLIEAG
                     YQPEIAYFECLHEVKLITDLIYEKGISGMRESISDTAEYGDLTRGRRIITEDTRDEMQ
                     MILEEIQSGEFAREWLLECQVNRPVYNAVKKQDASHLIEKIGAELRSMMPWIGKKK"
     gene            2224..>2609
                     /locus_tag="PKH07_09060"
     CDS             2224..>2609
                     /locus_tag="PKH07_09060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35132.1"
                     /translation="MANGEEHRESIQEFVESYVGIWSRLFTKPVAFFAEAVHEAGITP
                     IVVFAAINFVFAGVLAWLGVGKAELLVGFPLALLAGSLFCAAAYLVCLRTLKVQADYA
                     PLFRISLIITAAVAIPMAFPLLIIPAR"
BASE COUNT          565 a          671 c          737 g          636 t
ORIGIN      
        1 cattatcact gtcctttgtg tctttgagac tctgtggttg attgattgtg aagccttgct
       61 ccaggccttc ggggctattg acgtaccatt cggtcacgtt gccacggttg aactcgaagc
      121 gattgcccgt tgccttcctt ccctcgacag gtggaatcgg cgcgtttccc aattcatccc
      181 tcatccttcc gccttcatcc ttcgtgcatc ccaccagttc caagccccat tcccatgatg
      241 ggttggtttc cgtgcggcgc acaacgcgga tgccgctatc ggtgaagtac gttcggaggt
      301 tttgtttgcg gttgggtgcc tgatagaagc cggtaatccc cccttgatcc ccccttagta
      361 agggggatgg aggaagaaga tccccccttg tcgaatcggg acaggaagag gtttcctcac
      421 cccgaccctc tcccccaagg gtcgaaggag cagcatcact ttgtaaggtg atgttgtatt
      481 ccgaggcggc aatgtcgttc tggacttggg ataaccaact atcaggaata tcctgtgccg
      541 caaaacccga tgaagcgatc atcactgcca gaagaagtcc tgttgcgatt tgttctgttc
      601 tcatggtgtt cctcccgtca acatcttccg aaagactcaa tccgaattgg gggctgctcg
      661 acaagcagtg agcgttcccc ttacaccagc gatgtccact catggggcga atccctgcac
      721 cgtctgaaaa ccgctagaat agtccatggt tgtccagcca atcgagtttt gtcaagtgcg
      781 ataccgatga aacaccagag tcagtctatt caggatatgt gaggcgatca tgacttgctt
      841 cgtcacagca cacccggatc cgcccatttc ccgcatctct ggaaatttgc acggcacaaa
      901 atcttcactt gtgctaggat gcccattgtt ccgtttgaca cgaaccttgg aatcaggcct
      961 caagattcga ggacgtgtca tcgcaacaac aaacaaagca aagcgcctgg gaagaggttc
     1021 caagcgccgc ctttggtttt tgtgactgaa ggcaaaagaa aaccctggag gggagaagaa
     1081 gttatggcaa ggatttatta cgacaaggac gcgagcctgg acattctgaa aggcaaaaag
     1141 atcgctgtca tcggttacgg cagccaaggt catgcccagg cgcagaatct ccgagacagt
     1201 ggcttgacgg tcatcgtgtc agaactggaa ggaaccgcca actatgatct cgcggtcgag
     1261 catggcttca agccgatgcc tgccgcagaa gcagccaagc aggcggactt gattcagatt
     1321 ctggttccgg atcagacgca ggcgatgctg tatcgcggtg cggttcagca ggcgatggga
     1381 ccgggaaaga ccctgttgtt ttcccatgga ttcaacattc attttggcca gatcgttccg
     1441 ccggcgaccg tggatgtcat catggttgcg ccgaagggac ctgggcatct ggttcgccgc
     1501 gtttacactg agggtggtgg ggttccgtgc ctgatcgccg ttcagcagga tgcgtccggc
     1561 aaggcaaaag aaaccgcact ggcatacgcc aggggcattg gcggcactcg tgctggcgtt
     1621 ttggagacca ctttccgtga ggaaaccgaa accgatttgt ttggggagca ggtcgttctc
     1681 tgcggtggcc tgaccgagtt gattcgcgct ggattcgaga cgctgatcga agcgggctat
     1741 cagccggaaa tcgcctattt cgagtgcctg catgaagtca aattgatcac cgatctgatt
     1801 tatgaaaagg gcatctcggg gatgcgtgag tccatcagtg acacggcgga atatggtgac
     1861 ctgacccgcg gtcgtcggat catcaccgaa gatacccgcg atgagatgca gatgatcctt
     1921 gaggagattc agtccggtga gtttgctcgc gagtggctcc tggagtgtca ggtcaaccga
     1981 ccggtctaca atgctgtcaa gaaacaggat gccagccatc tgatcgaaaa aatcggcgcg
     2041 gaactgcgtt cgatgatgcc gtggatcggc aagaagaagt agttttttct ttcggacaaa
     2101 cggagaggaa gacaaggcgt ccaccgttgt gcagtcgttt ggcgtctgtc cgtgttggag
     2161 actgcttttc ttcctctctg tttgagagaa tcaggcaaca gccggaaagc gaggacatgg
     2221 atcatggcca atggtgaaga gcatcgggag tctatccagg aatttgtgga atcgtatgtg
     2281 gggatctgga gtcggttgtt caccaaacca gtcgcctttt ttgcagaggc ggttcacgaa
     2341 gccggaatca cgccgattgt cgtctttgca gcgatcaact ttgtgtttgc gggtgttctg
     2401 gcgtggcttg gtgtaggaaa agcagaacta ctcgtcggct ttccgcttgc cctgttggca
     2461 ggttctctgt tttgcgccgc agcctatctg gtctgtctgc gaacgttgaa agtccaggcg
     2521 gactatgcgc cgcttttccg catctccctg atcatcacag cggcggttgc cattccgatg
     2581 gcatttccat tgctgattat ccctgctcg
//
LOCUS       DAOQUC010000483         2609 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_47475_length_2609_cov_3.129209, whole genome shotgun sequence.
ACCESSION   DAOQUC010000483 DAOQUC010000000
VERSION     DAOQUC010000483.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2609
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47475_length_2609_cov_3.129209"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(115..1902)
                     /locus_tag="PKH07_09065"
     CDS             complement(115..1902)
                     /locus_tag="PKH07_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase domain-containing protein"
                     /protein_id="HNT35133.1"
                     /translation="MQNTESLVAILSERGRRKLPVERIYRQLYNEDLFLTAYGNIYGN
                     KGATTPGSDPQNQVDGMSLERIRRIIESLRNGSFQWKPVRRNYIPKKNGKMRPLGIPN
                     WDDKLVQEVMRILLEAYYEPQFSSHSHGFRRRKGCHTALEEIARTFKGSVWFIEGDIK
                     GCFDNIPHEKLAEVIRNNVHDERMVALIQGLLKAGYLEDWKWNATYSGTPQGGIISPL
                     LANIFLDQLDQFIETKLMPIYNRGTVRRQNPPYRIAQKRARRAFSAGRTEEGLYYRKI
                     QRATPSGDPMDKTYRRLGFVRYADDFILGFAGPKSEALAIKNEIGQFLASIGLELSEE
                     KTKITHARTEKARFLGYELLVSQNDTKQTSQTDGIRRRSVNGAIWFGIPQDTVHKWKR
                     KYSRNGKPIHKGEMVADSNFDIVSRYQMAFRGLAQYYSWAHNRSKTLWPLQWVMSRSL
                     TSTLADKHKTSCKAIVDKLKTRMVVEGKEFVVLKVIVKRDHGKKPLETHWGGISLARN
                     PAIIRDDPNHYLLHQGRTELVQRLLADKCEICGSRESVQVHHIRALKDLKKRKGREPL
                     PHETLMAARQRKTLVVCLECHQKIHSGKL"
BASE COUNT          492 a          666 c          614 g          837 t
ORIGIN      
        1 gcgaggtact cagcggagtc cttttccgac cgcccctctc agaaccggac gtgcatcttt
       61 caacgcatcc ggctcgccag taagtcgttt tgtatactct gtatcccatt gccattacag
      121 tttcccgctg tggattttct gatgacattc caggcatacg accagggtct tccgttgtcg
      181 tgcagccatc agtgtttcat gtgggagagg ttctcggcct ttacgtttct tcaggtcttt
      241 caaggctctg atgtggtgta cctgaacgga ttcgcgtgat ccacagattt cacacttatc
      301 cgccagtagc ctttgcacca gttccgttct cccctgatgg agcagatagt ggttggggtc
      361 gtctcttatt attgcggggt ttctggctaa agagatgccg ccccagtgcg tttccagcgg
      421 ctttttacca tgatctcgtt tcacgataac cttgagtacc acgaattctt ttccttcgac
      481 taccattcgt gtcttgagtt tgtcaacaat ggccttgcag gatgttttgt gcttgtccgc
      541 cagagtgctt gttagcgaac ggctcattac ccattgaaga ggccagaggg ttttgcttcg
      601 gttgtgcgcc catgagtagt actgggccag ccctcgaaac gccatttggt atcttgaaac
      661 aatatcaaag ttgctatccg ctaccatttc ccctttatgg atgggtttac catttctgga
      721 gtatttcctt ttccatttgt ggacagtatc ttgtgggata ccaaaccata tagctccatt
      781 cacactccta cgtctgatgc catcggtttg cgaggtttgt ttggtgtcgt tctggcttac
      841 caaaagttca taccccagaa accttgcttt ttctgttctg gcatgagtga tcttggtttt
      901 ctcttcggaa agttcaagac cgattgaagc gagaaactgt ccgatctcat tcttgatcgc
      961 caatgcttct gattttgggc cagcaaatcc caatataaag tcatcggcat atctgacaaa
     1021 ccccaatctt cggtaggtct tgtccatagg atcgccgctt ggtgttgccc gctggatttt
     1081 ccgatagtac aagccttctt cggttcgccc tgcgctgaaa gcccgtctag ctcttttctg
     1141 tgctatccta tatggtgggt tttgtcgccg tactgttcca cggttataga taggcatcag
     1201 ttttgtttca atgaattggt ctaactggtc gaggaaaatg tttgccagta atgggctgat
     1261 gatgccgccc tgcggtgttc cgctatacgt ggcgttccat ttccagtctt ccaggtaacc
     1321 ggcttttagc aaaccctgaa taagtgcgac cattctttca tcgtgaacat tattccgaat
     1381 tacctcagcc agtttttcat gcgggatgtt gtcaaagcat cctttgatgt ccccctcgat
     1441 gaaccagacc gatcctttga atgttcgggc tatttcttct agggctgtgt gacagccctt
     1501 tctgcgtcga aagccgtgtg agtgtgagct gaactgtggt tcgtagtacg cttcgaggag
     1561 tattctcatg acttcttgaa ctagcttgtc gtcccagtta ggaattccaa gcgggcgcat
     1621 cttcccgttt ttcttgggaa tgtagttgcg tcggactggt ttccactgaa acgatccgtt
     1681 cctgaggctt tcaatgatcc ttcgaatcct ctctagcgac atgccgtcca cttggttttg
     1741 tggatcgctt cctggagtgg ttgccccttt gttcccgtat atgtttccgt aggcggtcaa
     1801 gaataggtct tcattgtaga gttggcggta tattctttcg actggaagct ttcgccttcc
     1861 ccgttcggat agtattgcta cgagtgattc ggtgttttgc atttcgcaat tgcctcctca
     1921 ttggggcgtt tgtgagattc agagagttgt tgacttaccg tttttctcat cgagaaactg
     1981 cacaggtccc gtgcttgtgt tctctttgat actgggcctc tttgcccttt ccacaggttt
     2041 acttgttcac ctgggtaccc tattgcaggg gttggttcgg ctggcacaga gagccttctg
     2101 tgccttttct accaactgac tactataggc cctccgtcgc cataggtctc tcgaccctta
     2161 ggcgatcccg tagttgcgtg agtgagacgt atcgagtgga atttaggatg tcttccgatg
     2221 cgttgacaac ctttatcggt ctgccttctg gatctgagca ttcctgccac aatgtaccat
     2281 tggcagtatt ccagaactac gccgttagat gtgttggcaa ggggtctcac attatcccgg
     2341 tagcatctcg acttcggaca ttgaccgtcc tatccgtatt ccacaggtct tgcggagcaa
     2401 tagattacac atcttcctct gctctccgct tttcaagcat gcttctttca gccattcctt
     2461 tcggtttgac ctgagcttga tgacctatag gttgttgtgc tcagagacct atacttattg
     2521 cataagcaat cctcctcacg ccacaacggc gcactatatt tgggagccgt caatgcctac
     2581 ggcttccacc gcttcgccgt caggctgtt
//
LOCUS       DAOQUC010000484         2608 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_47501_length_2608_cov_3.544849, whole genome shotgun sequence.
ACCESSION   DAOQUC010000484 DAOQUC010000000
VERSION     DAOQUC010000484.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2608
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47501_length_2608_cov_3.544849"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..446
                     /locus_tag="PKH07_09070"
     CDS             <1..446
                     /locus_tag="PKH07_09070"
                     /inference="COORDINATES: protein motif:HMM:NF013595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HNT35134.1"
                     /translation="SGGLQGEWGSKGTRSGKFDEPVGIAAGPNGHIYVADTGNKRIQV
                     FESSEPYRCVAVWNVPGWSLLTSEPYLAFGPDNCLYATDAGSGRILRFSPDGQKADIY
                     SLPPGGGAELTRPTGIAVASDGTIFVSDRERAQILKVRPVEISEQ"
     gene            574..1050
                     /locus_tag="PKH07_09075"
     CDS             574..1050
                     /locus_tag="PKH07_09075"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNT35135.1"
                     /translation="MNIVRIRTAEFGDIPSVVSLWRGMLDFHYLRDGRFELAEDADAL
                     FASHLEQALLRNDHRVAVALREAQVVGYVHAAFLENLEMFRIRRYGLLSDLAVAPEMR
                     RQGIGMALWHAAQQWFSEQGISSVQLNVSEANPEAQEFWRACGFREFLKVLWCDLA"
     gene            1147..1521
                     /locus_tag="PKH07_09080"
     CDS             1147..1521
                     /locus_tag="PKH07_09080"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HNT35136.1"
                     /translation="MKHLMPSTLCLLLLTGCLHSYVNVASDPPGARVFFNHEDKGKTP
                     AKIEFLWYGKQKIQLFKEGYQRKDEIVDVKCPPHLYFPLDLFFTAMPFKFEDNHHFTY
                     TLTPNVPEPQEAPKAVQETPQQ"
     gene            1609..>2608
                     /locus_tag="PKH07_09085"
     CDS             1609..>2608
                     /locus_tag="PKH07_09085"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNT35137.1"
                     /translation="MVDSRPRVLVVDRETEETKRLLAYLERERYETLWARDGEAGFNV
                     LDSPSPPEALISEIRLHRINGMRLLELARSRRSDICAVMIATAAEIDLAIEAMRQGAL
                     DFQVKPLNLDKLALTLERGLAHQRLVWELSDLRYRFQEKEGFHGLIGISPGIRQLFET
                     LEQVATTNAAVLIIGETGTGKDSIARAIHEASERRNAPFVKLSCGALAESLIETELFG
                     HERGAFTGAVSPRAGRFELADGGTFFLDGVDELPLQTQGKFLRILEKREFEPLGSTVS
                     RKVDIRIIAAARQDLGELVQKGKVREDLYYRLRVVQLPIPPLREQKEDIPPLVSHFL"
BASE COUNT          596 a          667 c          761 g          584 t
ORIGIN      
        1 gttcaggcgg actgcaggga gaatggggca gtaaaggaac ccgctcaggg aaattcgatg
       61 agcccgtcgg aattgcagcc ggcccgaacg gacatatcta cgtggcagac accggcaaca
      121 agaggattca ggtctttgag tcttcagagc catatcgttg tgtggccgtc tggaacgttc
      181 caggctggag cctactgaca tccgaaccgt atcttgcctt tggacccgac aactgtctct
      241 acgccacgga tgccggatcg ggaaggattc tgcggttctc tcctgatggt cagaaagcgg
      301 atatctactc tctgcctcca ggtggtggag ccgagttgac ccgacccaca gggattgccg
      361 ttgcttctga cggtacgatt tttgtgtccg accgagaacg agcacaaatt ctcaaggtgc
      421 gaccggtcga gatctcagaa cagtagacct tcgcctcaca tgcgtactgc gcgagggatt
      481 ctcgacacga ttgtcgagca agcatccgag ggagcggcat agcacacgcg cctgcaggtg
      541 ctaatctgct caggatgatg gggcgtatcg ttcatgaaca tagtccgcat ccgaacagcc
      601 gaatttggag acattccctc cgtcgtgagt ctgtggcgcg ggatgttgga ttttcactat
      661 ctgcgcgacg gacgatttga actggcagag gatgcggatg ccctttttgc gagccatctg
      721 gaacaggcgc tgcttcgaaa cgatcatcgg gtggcagtcg cgctcagaga ggcacaggtt
      781 gtgggttatg tacatgcggc gttcttggag aacctggaga tgtttcggat tcgccgctac
      841 gggctgttgt cagatctggc agttgcaccg gagatgcgtc gtcagggaat cggaatggca
      901 ctatggcatg ctgcgcaaca gtggttctcc gagcaaggca tctccagcgt tcagttgaat
      961 gtttctgagg ctaatccgga agcacaggag ttctggcgag cgtgcggttt tcgggaattc
     1021 ctgaaggtac tttggtgcga cttggcatga gtcaacgcga tgcgctatct tttttgcctc
     1081 gcttgacttt gcgaaagcca attggtaggt ttcactgcga accctaaacg gagggaaata
     1141 ggtgctatga aacatctgat gccttcgacc ctttgtctgc tgttgctgac tggctgtcta
     1201 catagctatg tgaatgtggc cagcgatccg ccgggtgctc gcgtcttctt caaccatgag
     1261 gacaagggca agacacccgc gaaaattgag tttttgtggt atggaaagca gaagatccag
     1321 cttttcaaag aaggttatca acgcaaggac gagattgtgg atgtgaaatg tccgccgcat
     1381 ctgtatttcc cgttggactt gttcttcact gccatgccgt tcaagttcga ggacaatcac
     1441 catttcacct acaccctgac gccgaacgtt ccggaacctc aggaggcgcc caaggcagtt
     1501 caggaaacgc cgcagcagta ggtgactccc atcgtcccct tgtaggaatg ctccccggac
     1561 tgaacggttc acgggttcgg tcacgggtgt cgttcgagga gttcctcagt ggttgattca
     1621 agacccagag tgcttgtcgt ggatcgggaa accgaggaaa ccaagcgtct gctggcctat
     1681 ctggaacgag aaagatacga aaccctctgg gcaagagacg gtgaggcagg tttcaacgtc
     1741 ctcgactccc cttccccacc cgaagcactc atcagcgaga ttcgcctgca ccggatcaat
     1801 ggaatgagac ttctggaact ggcgcgttcc agacgctcgg atatctgtgc ggtcatgatc
     1861 gccacggccg cagaaatcga tctggcaatt gaggccatgc gccagggagc attggacttt
     1921 caggtcaagc cattgaatct cgacaaactg gcattgacgt tggagagagg acttgcccac
     1981 cagaggcttg tctgggaact ctccgatctt cgataccgat tccaggaaaa ggaaggcttc
     2041 cacggactga tcggcatatc accagggatt cgtcagctgt tcgaaacact ggagcaggtc
     2101 gcaaccacga acgccgcggt tttgatcatc ggggaaaccg gcactggcaa ggacagcatc
     2161 gcgcgagcga tccatgaagc cagtgaacgc cgcaatgctc cttttgtaaa gctcagttgt
     2221 ggtgcacttg cggagagtct tattgagacg gagctgttcg gccacgagcg gggagcgttc
     2281 acgggagctg tctctccgag ggcaggacga tttgaactgg ccgacggggg aacattcttt
     2341 ctggacgggg tggacgagtt acctctgcaa acccagggca aattcctgag aatcctggag
     2401 aagagagaat tcgagcctct tggaagcacg gtatcccgca aagtggacat tcggatcatt
     2461 gccgctgcac gccaagactt gggagaactg gttcagaagg ggaaggttcg agaagacctg
     2521 tactatcggc tccgagtggt tcaacttcca attccccctt tgagagagca aaaagaagac
     2581 atcccaccac tggtctcaca ctttctca
//
LOCUS       DAOQUC010000485         2605 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_47583_length_2605_cov_3.207451, whole genome shotgun sequence.
ACCESSION   DAOQUC010000485 DAOQUC010000000
VERSION     DAOQUC010000485.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2605
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47583_length_2605_cov_3.207451"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="PKH07_09090"
     CDS             <1..217
                     /locus_tag="PKH07_09090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35138.1"
                     /translation="YHMPETNHFIIPALSSLPAASEKVQGAAEGPAKVWRIYLLSLCA
                     LVLPIALWRLIVAYQKERNRTKTVFSG"
     gene            complement(620..>2605)
                     /locus_tag="PKH07_09095"
     CDS             complement(620..>2605)
                     /locus_tag="PKH07_09095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35139.1"
                     /translation="HPDNSQRPFRTSPILIGLLASAAVWIVTPYNNFVILGAFVSDSY
                     LPIAGFTALLIIVLLANPMLRRYMPAIAYSEKDFALILSMILIASVLPGQGLLRTVPY
                     SLVGLAVHVRENARFAEFFATMRLPSVLFPETMAFGVATPVADAFVNELSPGDSIPWG
                     AWVVPVLVWGVFFICCFLLMIGLALILFPQWRNNERLSFPLLAVHQSLIEDPKEGSFF
                     SALFKERSFWLAAVLVFIIHQFAGWQLQNPEGVPAFPVSWTLRRLFLDGPLRYLPAHI
                     HTNRLYFVFLGIAYFMPSRIGFSIWFFAVAYGFYVVAGNTLFPPYYSMTINDHRFGAM
                     LALSLWVVWLGRAHWAEIIQCMIRKPLDAEGRRNRQAGMMFLTGCVGMFLWLVWVGVQ
                     PAWALVFIVLGFMISLTITRLVAETGMPFIRIDMAHQMSFLRLFPNSWLTGTSLLFSY
                     FIVMVFAIGSRVSCTTMAAHAIGLHERESPSVQSRLCWLLVGVLAIGLIIGWGASLTA
                     NYHYSASINGEERPVCVYAYGIVGKIVTALQDFSQGRFTPPPYRQILHIGFGFAAVAL
                     LQWASLVMPTWPIHPIGFVMINTYYMNEAWASVFLGWLIKILILKYAGARAYRAARPF
                     FLGLIVGELIAGAFWNVILGVLVLLGMSYTRVKIQPY"
BASE COUNT          699 a          680 c          716 g          510 t
ORIGIN      
        1 ataccatatg cccgagacga accatttcat cattcctgcg ctttcgagtc ttcctgctgc
       61 cagcgagaag gttcaggggg ctgctgaagg tcctgccaaa gtgtggagga tctacctgtt
      121 atcgctgtgt gcacttgtgt tgcccattgc tctgtggagg ctcattgtgg cctatcaaaa
      181 agagagaaac cgtacaaaga ctgtcttcag tggatgacgc ggatttcaga gattcatccg
      241 tagtgcggca ttcatgccgc gatttcgttt gcagttacgc cgctaggcgt gatcttctcc
      301 cgtttgtagt taccacgtca ggggtgatcc ggatatgccc ttgcgggcac actgcaaacc
      361 tttgggcgat gcgttcttcg taagagttca acctttaggt tgctccggaa aaagacacgc
      421 taaagcgtga actcttacaa aatacagtgt cggatcctca gtgtctcact actagcttct
      481 ccttcaggag aaggagtccc cctgggtgcg ccaagtgtgc acttggctct tcttttttcg
      541 aactgccgag ctgacgctct gcaatcccag ggttgtctgg cttcggccgt gctattcgca
      601 gaaagccatc ttcccaagat caatacggct gaatcttcac tcgtgtgtag gacattccca
      661 agagtacgag gactcccaga atgacgttcc agaaagcacc ggcgatcagt tcgcccacga
      721 tgaggccgag aaagaaagga cgtgctgcac gatacgctcg agcaccagcg tacttgagaa
      781 tgagtatctt gatgagccat cccaggaaga cgctcgccca cgcttcattc atgtaatagg
      841 tgttgatcat gacaaagccg atggggtgaa tcggccaggt gggcatgacc agtgacgccc
      901 attgaagcag tgccaccgcg gcaaatccaa aaccgatatg aagtatctgc cggtatggcg
      961 gcggagtgaa tctgccctgt gagaaatcct gcagtgccgt gacgatcttg cccacgatgc
     1021 cgtaggcata gacacagacc ggtcgctcct cgccgttgat tgaggcgctg tagtggtaat
     1081 ttgcggtcag acttgccccc caaccaatga tcagaccaat cgccagaacg cccaccagaa
     1141 gccaacacag acggctctgc accgaaggag actctctctc atgaaggcct atcgcatggg
     1201 ctgccatcgt ggtgcaactg actcgggaac cgatggcaaa gaccatgacg atgaagtagg
     1261 agaacaggag cgacgtgccc gtcaaccagc tgtttgggaa caaccgaagg aaactcatct
     1321 gatgtgccat gtcaattcgg atgaacggca tcccagtctc ggcaaccaag cgggtgattg
     1381 tcaaagagat catgaagccc aggacgatga acaccagcgc ccacgctggt tgaacaccca
     1441 cccagacgag ccagagaaac attccgacgc atccagtcaa gaacatcatt cccgcctgac
     1501 gattccggcg gccttctgcg tcgagaggct ttcgtatcat gcattggatg atctctgccc
     1561 aatgggcgcg ccccagccag acgacccaca gcgaaagggc aagcatggca ccaaatcggt
     1621 gatcattgat ggtcattgaa tagtaaggtg gaaacagggt gttaccggca acgacataga
     1681 agccataggc aactgcaaag aaccagatgg agaaaccgat tcggctcggc atgaaatacg
     1741 caatcccaag aaagacaaaa tagaggcggt tggtatggat atgtgccggc agatagcgca
     1801 gcggaccgtc gagaaacaga cgtctgagcg tccaactgac ggggaaagcc ggaactcctt
     1861 cgggattctg caactgccaa ccggcaaact ggtgaatgat gaaaaccagc actgccgcaa
     1921 gccagaaact cctctccttg aacaaggcgg agaagaatga accttccttg gggtcttcga
     1981 tcagggactg atggacggcg agcaatggga acgaaagccg ttcattgttg cgccactgag
     2041 gaaagaggat caacgccaaa ccaatcatca ggagaaaaca gcagataaag aagacgcccc
     2101 agacaagaac aggaacaacc catgcccccc acgggatgga atcacccgga gagagttcat
     2161 tgacaaaggc gtctgcgact ggggtcgcca caccgaacgc cattgtttcc gggaagagaa
     2221 ccgaaggcaa tctcatggtt gcaaagaact cggcaaagcg agcattttcg cgcacatgca
     2281 ccgcaagccc caccagtgaa tagggaacag ttcgcaggag tccctgtccg ggcagtacgg
     2341 acgcaatgag aatcatggac aggatcagtg cgaaatcttt ctctgaatag gcgatggcag
     2401 gcatgtacct tctgagcatg ggattcgcca agagcacaat gatcagaaga gcagtgaagc
     2461 cggcgatggg cagataactg tcggagacaa acgcacccag aatgacaaaa ttgttatagg
     2521 gagtgacaat ccagactgcc gcagaggcaa gcagaccgat caggatgggg cttgttctga
     2581 agggacgttg gctgttgtca ggatg
//
LOCUS       DAOQUC010000486         2598 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_47778_length_2598_cov_3.519072, whole genome shotgun sequence.
ACCESSION   DAOQUC010000486 DAOQUC010000000
VERSION     DAOQUC010000486.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2598
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47778_length_2598_cov_3.519072"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..582
                     /locus_tag="PKH07_09100"
     CDS             <1..582
                     /locus_tag="PKH07_09100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35140.1"
                     /translation="DLIRVALASRRFYDLNGMYPPAVDDLIEAGLIGSVTSHFMMETN
                     VSEIQILDILGSETIPDPRIFAAERLLASSLLDHYLTVEASDDEIVLKGSRNKLREAG
                     GQSLAERDWFSEWSDALATLDPWFGEPTYIEHPDPFYSMASFVVPVNIPKHFWWAYSP
                     GPDGEETGGYPVYDWGHGPTSEGDIGCFVGCEW"
     gene            663..>2598
                     /locus_tag="PKH07_09105"
     CDS             663..>2598
                     /locus_tag="PKH07_09105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35141.1"
                     /translation="MIKKRSVFVNELHRRWKIAVFLLVADALLSACIGVTLYVSKQDQ
                     ASLMDTVTWFQFPYGLFLVTVLGVSAFERTEKDGAKFFLYHTPVSSTRVFWAKYVSGL
                     LLTLVLIASIAAVFAALRKTDTTLSQNPGTWVLIVPVVWCLYTVGFLVSPLFETDIVA
                     FGVALIQTALAMILAYTFWLKFPCLSPLIKGVNRWQFLFCPELLVVSGFLALGRLLFR
                     HFGCLRVSFRRRRWTLLMLMLAAIALSFCNTFIDFHDLFYLMTTRTKVHAANEETQRS
                     ALLAMAEKVDRYREEIYGPSCYADYLAIFGDESMVSYPRQQQVSWCSQRYRLLSRNPE
                     TRQMSEAYFHLRSPWWDKLREALACGMMYRLCTDMYMSPEEPEVDLLLVCYCIEDLCR
                     KALYDSSNELGIVSAYSLSLALELSSVIVTDNMAGFRSAYLARVVDTCADMLWRSPTP
                     EWISEVTLSFEETHPRAAHIDPPAVDRFITLVRYHQWILDLNEEAKGIKGIYSPINHI
                     RLHLLLSYLHAMQEGGSSSIPKWLSRLNENANDELWRTIPYQHESPYSMRLTTAPTLR
                     RRVAELSTGSTLVDRDLTPFEPADFEELDAETVAALTTDYLFSQGFEHTGRRDISEIF
                     THFDLIRVALASRRFYDLNGM"
BASE COUNT          590 a          653 c          664 g          691 t
ORIGIN      
        1 gatctcatcc gcgttgctct ggcttccagg cgattctatg acttgaatgg gatgtatccg
       61 cctgcggttg acgatctcat tgaggcggga ctcattggaa gtgtgacaag ccatttcatg
      121 atggaaacca acgtatccga gattcagatt cttgacatcc tcggatcaga aacgatccct
      181 gacccacgga tatttgccgc tgagcgactc ctagcatctt cattgttgga tcactatctg
      241 actgtggagg caagcgacga cgagatcgtc ctgaaaggta gtcggaataa gctcagagag
      301 gcgggaggac agtctttggc tgagcgagac tggttctcgg aatggagcga tgcccttgcg
      361 acgcttgatc cctggttcgg agaaccgacc tatatcgagc acccagatcc cttttacagc
      421 atggcgtcat ttgttgttcc ggtcaacatt ccaaagcatt tctggtgggc gtattctccg
      481 ggacccgacg gagaagaaac cggcggatat cctgtttacg attggggaca cggaccgaca
      541 tcagaaggcg acatcggatg ttttgttgga tgtgaatggt aagattgttt ttctggactt
      601 tcatcgtcca ggaaacatcc cgtgctgtga gagtgcgggc aacaactgca gagggaaaca
      661 agatgatcaa gaaacgtagt gtgtttgtga atgaactgca caggcgatgg aagatcgctg
      721 tgtttctgct tgtggcggac gctcttcttt cggcctgcat cggtgttacg ttgtatgtct
      781 ccaaacaaga ccaagcaagt ctcatggata cagttacatg gttccagttt ccttacggtc
      841 tctttctagt gaccgtgcta ggcgtttctg cctttgagag aaccgaaaag gacggagcca
      901 aattctttct ttatcacacc cccgtctcgt cgacgagagt gttctgggct aagtatgtat
      961 ccggtcttct tctgacgctc gtcctgatcg ccagcattgc cgctgttttt gcagcgctta
     1021 ggaaaacaga cactacactt tctcagaatc caggtacatg ggttctcata gtaccggtag
     1081 tctggtgtct ctatacggta ggattcctgg tctctccgct gttcgagaca gacatcgtcg
     1141 cgtttggtgt tgcgcttatt cagactgccc ttgccatgat tctggcatac accttctggt
     1201 tgaagttccc ttgcctatct cccctcatca agggtgtcaa tcgatggcag tttctcttct
     1261 gcccagaact cttggttgtt tcgggctttc ttgctcttgg cagattgctc ttccgacatt
     1321 tcggatgctt gcgcgtctct ttccgaagac gcagatggac gctgcttatg ctcatgcttg
     1381 ctgcaattgc tctgagcttc tgcaacactt tcatagattt ccatgatctg ttctatctga
     1441 tgaccacccg aacgaaagtg cacgctgcca acgaggaaac gcagcgcagt gcattacttg
     1501 cgatggcgga gaaagttgat aggtacagag aggaaatata cggtccgtcc tgctatgcgg
     1561 actaccttgc tatctttgga gatgagagta tggtcagcta cccaaggcaa cagcaggtgt
     1621 cttggtgctc ccaacgatat agactcttga gcaggaatcc cgagactcgt caaatgagcg
     1681 aagcgtactt ccacctccgt tccccatggt gggacaaact ccgagaagca cttgcctgtg
     1741 ggatgatgta ccggctttgc acagatatgt acatgtctcc agaagaaccc gaagtagacc
     1801 tcctcttggt atgctattgc atcgaagatc tctgcaggaa ggcattgtat gattcttcga
     1861 atgagctggg gatagtgagc gcatacagtc tctcgttggc tcttgaattg agctcagtga
     1921 ttgtaaccga taacatggcc ggattccggt ccgcatacct ggcgagggtt gtagatactt
     1981 gtgcagatat gctctggcga tctccgacac cggaatggat atcagaagtt actctctcat
     2041 ttgaggaaac ccatcctcgt gccgcgcata ttgatccgcc cgcagtggac agattcatca
     2101 cccttgtcag atatcaccaa tggattctgg acctgaacga agaagcaaag ggaatcaagg
     2161 gtatttattc tcccattaat cacatccggc tacatcttct gttgagctac ctccatgcaa
     2221 tgcaggaagg aggcagctca tccattccga agtggttgtc ccgtctgaac gagaacgcca
     2281 atgacgaact ctggcggacc ataccgtatc agcatgaatc cccttactca atgcgtctga
     2341 caacagcccc aaccctgaga aggcgggttg cggaactgag cacaggctcc actctagttg
     2401 accgggactt aactcctttc gaacctgcag actttgagga actggatgcc gagactgtgg
     2461 ctgctcttac gacggactat ctgttttccc agggctttga gcataccggg cggcgcgaca
     2521 tctcagaaat cttcacacac ttcgatctca tccgcgttgc tctggcttcc aggcgattct
     2581 atgacttgaa tgggatgt
//
LOCUS       DAOQUC010000487         2597 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_47814_length_2597_cov_3.219512, whole genome shotgun sequence.
ACCESSION   DAOQUC010000487 DAOQUC010000000
VERSION     DAOQUC010000487.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2597
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47814_length_2597_cov_3.219512"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1332
                     /locus_tag="PKH07_09110"
     CDS             <1..1332
                     /locus_tag="PKH07_09110"
                     /inference="COORDINATES: protein motif:HMM:NF039847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNT35142.1"
                     /translation="EREEGANKGRTEFSGRRTFIKTSLAAVTGTMLAPTFIPARALGL
                     EGRPAPSDRITLGFIGVGSMGSGNLRGFRSASDTQVLALCDVDRRRLASAKAETESFY
                     AQQQTDGTYHGCSDYVDFRQLVARQDIDAVVISTPDHWHVLPSIAAVRMGKDVYCEKP
                     LSLTIAEGRALSDAVKRHGRVFQTGSQQRSSQNFRLACELVRNGRIGKLHTIEVLLSS
                     GKQTTVQPPQPVPAELEYNLWLGQAPEEPYTSMRCHYNFRFIRDYSGGQLTNWGAHHL
                     DIAQWGHGSDHSGPVQVQGTGTFPSSGLWNNPTQYQIEYTYSDGVKVICKTSGSSVRF
                     IGTEGEVYVSRERLSAKPLSILESPIASNEIHLYDSRNHHRNFLDCIKTRRETAAPVE
                     TAHRTITMAHLGNIAMLLGRTLKWNPDTERFVDDPGADRMLSRSMRKPWHL"
     gene            1392..2201
                     /locus_tag="PKH07_09115"
     CDS             1392..2201
                     /locus_tag="PKH07_09115"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNT35143.1"
                     /translation="MVHLKSVALKAPLDDSDREFPYSVPVIKAFQKIEFTSPVTFFVG
                     ENGSGKSTFLESLACAIGSVTAGAKSVESDETLQAVRAFSKRLRLTWQLRTRKGFFMR
                     AEDFFGYAKRMAQERIELINDLRDAESRFKDRSQTAYDHARAPYAKEIGLIEKYYGEG
                     LDVGSHGECFLKLFKARFVPGGLYLMDEPEVPLSPLRQLSLIAMLKEMVQEENAQFII
                     ATHSPILLAFPEAIILSFDSGRVHAVDYESLEHVTLTRAFLNNPEAYLRHL"
     gene            complement(2212..>2597)
                     /locus_tag="PKH07_09120"
     CDS             complement(2212..>2597)
                     /locus_tag="PKH07_09120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35144.1"
                     /translation="RSTKAPMASRPWIWSDAAWHHPDEFLSRMPKSVVQSNWYYGMDF
                     TEENVAYKTWLLLAENGFDQIPCGSTWIHEKNFPTIVESLSERLGGQHLLGFMQSVWK
                     PMLEECRTTQVHALDMLRQAHSLVP"
BASE COUNT          589 a          714 c          687 g          607 t
ORIGIN      
        1 gagagagaag aaggcgcaaa caaggggcga accgagtttt ctggtcggcg caccttcatc
       61 aagacgtcgc ttgcagccgt cacgggtacg atgctcgccc ccacgttcat cccagcaagg
      121 gctttggggt tggaaggccg accggcaccc agcgatcgga tcactctcgg attcattgga
      181 gttggaagta tgggatccgg caatttgcgg gggttccgaa gtgcatctga tactcaggtg
      241 cttgcgctgt gtgacgtgga tcgcagacga ctcgccagcg ccaaggctga gacggagtcc
      301 ttttatgcac agcaacagac cgacggaacc tatcacgggt gctcggacta tgttgacttc
      361 cgccagctgg tggcgcgtca ggatattgac gcggttgtga tctcaacgcc cgaccattgg
      421 catgtgctcc caagcatcgc cgcagttcgt atgggcaagg acgtttactg tgagaaaccc
      481 ttgtctctga ccatcgccga gggacgtgca ttgagcgacg cggtcaaacg ccatgggaga
      541 gtttttcaaa ccggaagcca acagcgctct tcacagaatt ttcggctggc gtgtgagctg
      601 gtacgaaatg gccggatcgg gaaacttcat actatcgagg ttcttctctc ttccgggaag
      661 cagaccaccg ttcagccacc tcagcctgtt cctgccgagt tggagtacaa tctttggctg
      721 ggacaagctc ccgaagaacc ctatacttca atgcgctgcc actacaattt tcgattcatc
      781 cgtgactatt ccggtggcca gttgaccaat tggggcgcgc atcatctcga tatcgcacag
      841 tggggacacg gttcggatca ctccggtccc gttcaggtgc agggaaccgg tacctttccc
      901 tcaagcggtc tttggaacaa tcccacgcaa taccagatcg aatacacgta ctctgatggc
      961 gtcaaagtca tctgcaagac cagcggttcc agcgtccggt ttatcggcac agagggagaa
     1021 gtctacgttt ctcgcgaacg cttgtccgca aagcctctca gcattctcga atctcccatc
     1081 gcttccaatg agattcatct ctacgacagc agaaaccatc accggaattt cctggactgt
     1141 atcaagaccc gtcgtgagac tgctgcgccg gttgagaccg cccatagaac catcacgatg
     1201 gcacatctgg gcaacattgc catgcttttg gggcgtacgt tgaaatggaa tcccgataca
     1261 gagaggtttg tggatgaccc tggtgccgac cgcatgcttt ctcgaagtat gcgtaagccc
     1321 tggcaccttt agtgcctcag ttctctgagc gttttctcag tatgcctgtg tcactgtgca
     1381 gaatgcaaaa aatggttcat ctcaagagcg tcgcactcaa agccccattg gacgattccg
     1441 atagagaatt cccctattct gtacctgtga tcaaggcgtt tcagaagatt gagtttacct
     1501 caccggtcac cttcttcgtt ggtgaaaacg gctcaggcaa gtccaccttc ctggaatctc
     1561 tggcatgcgc tatcggttct gtaacggctg gagcaaaaag cgttgagagc gacgagacgc
     1621 tccaggcggt tcgcgctttc tcgaagaggc tccgcctgac ctggcagcta cgaacccgaa
     1681 aaggcttctt catgcgcgcg gaggacttct tcggttacgc aaaacgaatg gctcaggaac
     1741 gcatcgaatt gatcaacgat ctccgagatg ccgaatcccg cttcaaggat cgttcgcaaa
     1801 ccgcttatga tcacgcgcgt gctccctacg ccaaagagat tggattgatt gagaagtact
     1861 atggtgaagg actggatgtt ggctcacatg gagagtgctt tctcaaactc ttcaaggcgc
     1921 gctttgttcc tggcggactg tatctcatgg atgagccgga agtcccactt tctccactgc
     1981 gacaactgag tttgatcgcc atgttgaagg aaatggtgca ggaggagaac gcgcagttca
     2041 tcatcgccac gcattcaccg atccttctgg cgtttccaga ggccatcatt ctgagtttcg
     2101 actcaggacg cgtccatgcg gttgactatg agagcctcga acatgtcaca ctgacgcgag
     2161 cgttcctgaa caatcccgaa gcgtatcttc gacacctcta gacagacgca actagggcac
     2221 cagggagtgt gcttgtcgaa gcatgtcaag cgcatgaacc tgtgttgtgc gacattcctc
     2281 cagcattggc ttccagacac tctgcatgaa tcccaacagg tgctgtccac caagtcgctc
     2341 tgagagtgat tccacgattg tcgggaagtt cttctcatgg atccacgtgc ttccgcaagg
     2401 tatctggtcg aaaccgttct cggcaagaag cagccaggtc ttatacgcta cattctcttc
     2461 agtgaagtcc attccgtagt accagttgct ctgaaccacg gacttcggca tccgagataa
     2521 gaattcatcg ggatgatgcc aggcagcatc cgaccagatc catggcctgg acgccattgg
     2581 tgccttcgta gatctgg
//
LOCUS       DAOQUC010000488         2596 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_47855_length_2596_cov_3.002361, whole genome shotgun sequence.
ACCESSION   DAOQUC010000488 DAOQUC010000000
VERSION     DAOQUC010000488.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2596
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47855_length_2596_cov_3.002361"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /locus_tag="PKH07_09125"
     CDS             complement(<1..440)
                     /locus_tag="PKH07_09125"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase, FolE2/MptA family"
                     /protein_id="HNT35145.1"
                     /translation="MPKKQETVELRDVQSQPAEHPVVIHKVGVRSVPFPLVVMDKEHE
                     VQHTVALINMYVDLPKQFRGTHMSRFIEILNEYRGVFVVRNIYAILKDIKKRLKAESA
                     HLEASFSYFMNRKAPVSGSAGLMEFRCQFIASLNGEGRRDFVLV"
     gene            complement(533..1945)
                     /locus_tag="PKH07_09130"
     CDS             complement(533..1945)
                     /locus_tag="PKH07_09130"
                     /inference="COORDINATES: protein motif:HMM:NF015894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HNT35146.1"
                     /translation="MSERAEIKKVVYNEGGVQLKTSHDGLCAYVRFLENYPGNLDLRS
                     FLMLLQQQSIRNGLLPDAIKKSLESKDRDTDILIARGTPPVHGINGSVKYTIPPDAIR
                     AAPRLNPDGKVDMKELQVLHNFHTNDVLAELIPHTLGIAGKSVSGEPIEPQPGKPATL
                     TVGKGTMVSPDRTKLVAANDGALVYHEGIICIYPVFTVKEDVDYSVGNLDFVGTIFVK
                     GNVLSGFRVKAEGDISIDGLVEAAQIFAGNEIVIRGGVQGGGRAIIEAGGSIYANFIN
                     DAKLRAGEKIETRGSIMRCEVFAADEIVSPSKEGEIVGGKVIAGRKVQVQNLGTALGV
                     TTFIEVGTDPSIVSRLETLQALLAEDQEAFKQIAQTVDKIRTLERRIGRLPSRNQREF
                     EAALNNLNTLRANIELGQNEITRLEQQLEAMKESSVCVLGTVHPGVVIKIGKSTFKVA
                     EAMQAVRFQLQDDRIRISAL"
     gene            complement(2135..>2596)
                     /locus_tag="PKH07_09135"
     CDS             complement(2135..>2596)
                     /locus_tag="PKH07_09135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35147.1"
                     /translation="RKLTELAGMISQRLRTVRPGIHIAMYTWGPHVVSGHPVAQDWPT
                     WAKKGYIDSVNVSGYCFPDNYGDKYLEVYEQRLVLAKQMLSEASPSCEVTLCLGVKTS
                     HGEVKSADDINTYLEIAKKHGYRGVSFFTWSTLLKHLDGFQQNQYLGRFLE"
BASE COUNT          582 a          673 c          673 g          668 t
ORIGIN      
        1 tcgacgagca caaagtctct cctgccttcc ccattcaggg aggcgatgaa ctggcagcgg
       61 aattccatca gccccgcgct tccagagaca ggtgcttttc ggttcatgaa gtaggagaaa
      121 gacgcttcga ggtgagccga ttcggccttg aggcgcttct tgatgtcttt gagaatggcg
      181 tagatgttgc gaactacgaa aacacctcga tattcattga gaatctcaat gaagcgcgac
      241 atgtgtgttc cgcgaaactg tttgggaaga tccacgtaca tattgatcag ggcaaccgtg
      301 tgctggactt catgttcctt gtccatgaca accaatggaa aagggacaga tcgaacccca
      361 actttgtgga tgacaactgg gtgttcggcc ggttggctct gaacgtcacg caactctact
      421 gtctcttgct tcttcggcat accagatcct cactgaaaca accgtagcac aaacgcggct
      481 ttactgggaa gtcgcaaaaa aggggcagtg gtaccgtgcc tgtagaagag gtctagagtg
      541 cgctgattcg aatgcgatca tcctgcagtt ggaaacgaac cgcctgcatg gcctcggcaa
      601 ccttgaacgt gcttttcccg atcttgataa ccacaccggg atgcactgtg cctagcacgc
      661 atacggaact ctccttcatc gcttcaagtt gctgctccag tctcgtgatc tcgttctgac
      721 ccaactcgat gtttgctcga agcgtattca ggttgttcag agcggcttcg aactccctct
      781 gatttcggga aggcagtcgt cctatacgtc tttcgagagt tcttatcttg tccaccgttt
      841 gggcgatctg cttgaatgcc tcctggtctt ctgccaagag agcctgaagt gtctccaggc
      901 ggctgacgat gcttggatcg gtgcccacct cgatgaaggt ggtgacgccc agcgccgtgc
      961 ccagattctg cacctggact tttcgaccgg caatcacttt tccgccaaca atttcccctt
     1021 ctttggaggg ggaaacgatc tcatcggccg caaaaacttc acaacgcatg atggagccac
     1081 gggtttctat cttctcaccc gcacgcagtt ttgcgtcgtt gatgaaattg gcgtagatgc
     1141 tgcctccggc ttcgatgatc gcacgcccgc caccctgtac accgcctcgg atgacaatct
     1201 cattgccggc aaagatctgt gcggcctcta ccaggccatc aatagagata tctccctcgg
     1261 ctttgacgcg gaaaccggac agcacattcc ccttgacgaa gatcgtcccg acgaagtcaa
     1321 ggttgccgac cgaatagtcc acatcttctt tgacggtgaa gaccggatag atgcagataa
     1381 tcccttcgtg gtacacaagc gcaccatcgt tggcggcaac gagcttggtt cggtcgggcg
     1441 agaccatcgt tcccttgccc accgtgagtg tggctggctt gccaggttgt ggttcaatgg
     1501 gttcgccaga taccgacttt ccagcaattc ccagagtgtg gggaatcagt tctgccaaga
     1561 catcgttggt gtggaaattg tggagaacct gcagctcctt catatccact ttgccgtcag
     1621 ggttaagtct gggcgctgca cgtattgcat cggggggaat ggtgtacttg accgaaccgt
     1681 tgattccatg gactggtgga gtacctcgcg caatgagaat gtctgtgtcc ctgtccttgc
     1741 tctccaagga tttcttgatg gcatcaggga gaagaccatt gcgaatggac tgctgctgaa
     1801 gcagcatcag aaaggagcga aggtcaagat taccaggata gttctcaaga aaacggacgt
     1861 aagcacacag accgtcatga gaagtcttca gttgaactcc accttcgttg taaactactt
     1921 tttttatttc tgcccgttcg ctcatattgc agccagccga ttcggctcag atacatatca
     1981 gagaagcatt tgtcttgagg caaatgctct gcatcttaag attagaccac ttctttctca
     2041 gatttcaagc tttatgtttg ataaactgaa gtattttatt agagaggcgt tcataagtga
     2101 tctgactctc cttgagagat tgtctgtgag agccctattc caggaatctt cccagatact
     2161 ggttctgttg aaaaccgtcg agatgcttca gcaaggttga ccaggtgaag aaggagactc
     2221 ctctgtagcc atgcttcttg gcgatctcca gataggtgtt gatgtcgtca gcggatttca
     2281 cttcgccgtg actcgtcttc acacccaggc agagagtgac ttcacaggaa ggactggctt
     2341 ccgagagcat ctgtttggcc aggacaaggc gttgttcata gacctcaagg tacttatctc
     2401 catagttgtc tggaaagcag tagcctgaga cgttgacact gtctatgtag cccttctttg
     2461 cccacgttgg ccagtcttgg gcgacaggat gaccgctgac tacatgtggc ccccaggtat
     2521 acatggcgat gtgtattcca ggacggacgg tgcgcagtcg ttggctgatc attccggcca
     2581 gttcggtaag cttgcg
//
LOCUS       DAOQUC010000489         2595 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_47877_length_2595_cov_3.738976, whole genome shotgun sequence.
ACCESSION   DAOQUC010000489 DAOQUC010000000
VERSION     DAOQUC010000489.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2595
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47877_length_2595_cov_3.738976"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="PKH07_09140"
     CDS             complement(<1..192)
                     /locus_tag="PKH07_09140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HNT35148.1"
                     /translation="MVGYNWKPINDLPDHWNLLVQEDVQSLASVWNDQAKKIRDSSSF
                     HDFLERLKRRWAIETGIIEG"
     gene            complement(227..>2595)
                     /locus_tag="PKH07_09145"
     CDS             complement(227..>2595)
                     /locus_tag="PKH07_09145"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF004349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HNT35149.1"
                     /translation="RHDIGREEMIRRIWEWKQEHGDAILKQLEALGCSCDWDRTRFTL
                     DEGLSRAVREAFVRLYEEGFIYRGTYLVNWCPRCQTTISDDEVEYTDRNGSLYYMKYP
                     LVDAENQFITVATTRPETMLGDTAVAVNPKDERFHDLIGRKLILPLMNRRIPIIADPV
                     VEVEFGTGAVKVTPAHDPNDFLMGQRHGLEQINIFTETAVINENGGAYQGLDRYKARK
                     KVLADLETLGLLEKVNPYQGRVGVCYRCTTEIEPYLSLQWFLKMKPLMQEPIRAVREE
                     RVRFVPKNWENTYFAWVENIRDWALSRQIWWGHRMPVWYCNDCGEIVVSREDPTQCSR
                     CKSANLRQDEDVLDTWFSSSLWPFSTFGWPDKTPDLQVFYPTSTLVTAYDIIFFWVAR
                     MIMMGLKFMGDVPFRDVYITPLVFDEQGRKMSKSLGNAVDPIDLIERHGVDAVRFTLT
                     IMAAQGRNINLSEQRIEGYRNFCNKIWNAARLILSGVQEPLDFRVLPEINPEHDVYEA
                     WIVSRLQRTIDAVRKSLEGYRFNDACETAYAFFWHEYCDWYLELAKPALYGADPDKAQ
                     KARTVALWVLENMLRILHPVIPFITEEIWQKMHEIAGQKPSPESIMIAEYPKLDAERL
                     CENVEAHMDSLQQVCYHIRNIRGELGIAPGEALDVIFRITQPDAKTLLQTHLSALKSL
                     LNLSENIALGSADQAMPKAASTAAVPGIEIAVVWTEDVRRREIERLQKILAKAEQEII
                     SRTVKLQNEKFVSRAPDHVIQKEKDALQTAQDEKEMVSKRLEGLKNVS"
BASE COUNT          578 a          711 c          677 g          629 t
ORIGIN      
        1 tccttcgatg attccggtct cgattgccca tcgtcgcttc aaacgttcaa gaaagtcatg
       61 gaaagatgag gagtctctga tcttctttgc ctggtcgttc cacaccgacg cgagactctg
      121 tacgtcctcc tgaacaagca gattccagtg atcaggcaag tcatttatcg gtttccagtt
      181 atatcctacc atgagcatac ctccctgatc atcggtcaca gagctctcat gacacgttct
      241 tcaggccctc aagccgtttt gagaccattt ccttttcatc ctgtgctgtc tgcagtgcat
      301 ccttctcctt ctggatcaca tgatcgggtg cacgagagac aaacttctcg ttctgcagct
      361 tgactgttcg agagatgatc tcctgctcgg cttttgccag gatcttctga aggcgctcga
      421 tctcgcgacg acgaacatcc tccgtccaga cgacggcgat ctcgatacca ggcacggccg
      481 cggtcgaagc agcttttggc atcgcctgat cggcacttcc caaggcgatg ttttcgctga
      541 gattgagcag gctcttcaac gcggagagat gcgtttgcag gagagtcttg gcatcgggtt
      601 gtgtgatgcg gaagatgaca tccaaagcct ctcccggagc gatgccgagt tccccacgaa
      661 tgtttcggat gtggtagcag acttgctgaa gggagtccat gtgcgcttcg acgttctcgc
      721 aaagcctctc cgcatcaagc ttcggatact cggcgatcat gatgctttcg ggagacggtt
      781 tctggccggc aatctcgtgc atcttctgcc agatttcctc cgtgatgaac ggtatcaccg
      841 gatggagaat tcgcagcata ttctccaaaa cccacagcgc cacagttcga gctttctgag
      901 ccttatcggg atccgcaccg tacagcgccg gctttgccag ttcgagatac cagtcacaat
      961 actcatgcca gaagaatgcg taggctgtct cacaggcatc attgaaccgg tagccttcaa
     1021 gagattttcg aacggcgtct atggttcgct gcaggcggga gacgatccat gcttcataga
     1081 catcgtgctc cggatttatt tctgggagca ctcgaaagtc gagaggctcc tggacaccgg
     1141 acagaatgag ccttgcggca ttccagatct tgttgcagaa atttctgtaa ccttcgatgc
     1201 gttgttccga gagattgatg tttcttccct gcgctgccat tatggtcaac gtgaagcgca
     1261 ctgcatccac tccgtgacgc tcgatgagat caatgggatc cacggcattg cccaaggact
     1321 tgctcatctt gcgcccctgc tcatcaaaaa ccaacggagt gatgtacaca tcacggaatg
     1381 gcacgtcacc catgaacttc aagcccatca tgatcattcg ggcaacccag aagaagatga
     1441 tgtcgtaagc agtcaccagc gtcgaagtgg ggtagaagac ctgcaagtcc ggtgtcttgt
     1501 ctggccagcc gaacgtggag aacggccaga gagaggatga gaaccatgtg tcgagaacgt
     1561 cttcatcctg tcgaaggttg gcgctcttgc agcgactgca ttgggtggga tcctcacgag
     1621 agacgacaat ctcaccacag tcgttgcagt accagacggg cattcgatgt ccccaccaaa
     1681 tctggcggga caatgcccag tctcgaatgt tctccaccca tgcgaagtag gtgttctccc
     1741 agtttttcgg aacaaaacga acacgctctt cacgaacggc acgaatcggt tcctgcatca
     1801 ggggtttcat tttgagaaac cactgcagag aaagataggg ttcgatctct gtcgtgcagc
     1861 gatagcatac accgacgcgc ccctggtaag gattcacctt ctcaagaagc cccaaggttt
     1921 ccaagtcggc gagcaccttc ttgcgagctt tgtagcggtc gagaccttgg taggcaccgc
     1981 cgttttcatt gatgactgca gtctcggtga agatgttgat ctgctccagt ccgtggcgct
     2041 gtcccataag aaagtcgttg gggtcgtgcg ctggtgtgac cttgacggct cccgtaccga
     2101 attccacttc caccacaggg tctgcgatga tcggaatccg tcggttcatc agcgggagaa
     2161 tcagttttct cccgatgaga tcgtgaaaac gttcatcttt cggattcacg gcaacggctg
     2221 tatcgccgag catggtttcg ggtcgggtcg tggcgacggt gatgaactga ttctctgcat
     2281 ccaccaatgg atacttcata tagtagagag agccgttgcg gtcagtgtac tcaacctcat
     2341 cgtcagagat cgtcgtctga catcgaggac accagttgac gagataggtg ccacggtaga
     2401 tgaagccttc ctcatagagt cgcacaaagg cttcacgcac agcccgggaa agcccttcgt
     2461 cgagagtgaa acgggtgcga tcccagtcac aggaacagcc caacgcttcc aattgtttga
     2521 ggatggcatc gccatgttcc tgtttccatt cccagatgcg ccggatcatc tcttcacggc
     2581 caatatcatg gcgcg
//
LOCUS       DAOQUC010000490         2588 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_48082_length_2588_cov_2.543624, whole genome shotgun sequence.
ACCESSION   DAOQUC010000490 DAOQUC010000000
VERSION     DAOQUC010000490.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2588
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48082_length_2588_cov_2.543624"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="PKH07_09150"
     CDS             <1..282
                     /locus_tag="PKH07_09150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35150.1"
                     /translation="KVSIRAVDGEGNKSAPVNLEIVDKTQKYELTVAQDLLGVVKASF
                     IFPEDPAGFIAVVRSLFKQGVRRGFLAKGVATELEETIVKVLERKTEKE"
     gene            1052..>2588
                     /locus_tag="PKH07_09155"
     CDS             1052..>2588
                     /locus_tag="PKH07_09155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35151.1"
                     /translation="MFLAEIGKSIKNSDDFERILDATSGLLSTEFRHMKEILAADVFP
                     VKREDVEAVRRKFQSCPEVGTSSLLQLDSLIAPPRPQLPQSSDQWGVEEWIRWAIRDY
                     MPYRHWQTKAKVFDAEVEQQAGHFTDWYLRQYSTLHQQASVSLTHVLSSLRDRLSADA
                     LALIVLLDGLPAIYWRHLSEALRVAGLHQVDSGWRLAPLPSHTALCKPLLLSGGWDDS
                     NQDYRAIVELRAQQEWGKRAIVYVSSLKELSDCQLSQEPSVVIFNFLSTDELLHADVE
                     SENATHEEKLHLLFVKLQETVVDLYKRWGGPIDQFSLYALTDHGACRVLGEEKQTLDS
                     KVVQKIFTEANHRFAEVGADEAQNVPQNLWGLGYRFQPPIAKVQDRCFFIPKGHNTVK
                     LPKQAKGYLHGGATPEEIIVPWVVWRTVRPAIKDLAARFLDLPVPIVFYVQRLTTLAV
                     EISNPNNQRVRVLEIRVVQPETDLVKFEPLDLEGKQSGQIAASMYFKKSALGRGILQL
                     ALDY"
BASE COUNT          600 a          682 c          746 g          560 t
ORIGIN      
        1 aaagtcagca tccgcgcagt ggacggagag gggaataaaa gtgctccggt gaacctggaa
       61 atcgtcgaca agacacagaa atatgaactg acagtggctc aagatctgct cggcgttgtc
      121 aaagcgagtt tcatcttccc tgaggatcca gctggattca tcgcagtggt gcggtccctg
      181 ttcaaacagg gtgtccgccg cggatttctg gcgaagggag ttgccactga attggaagag
      241 acaattgtaa aagtgttgga gcgcaagaca gagaaggaat gatctcacca tgatggcaag
      301 tccaacgatt cgagtgcgcc tggtcggggc ggaattgcct ttagatcccg cctccggttt
      361 cgttttggag tccaaccgtg attatgtccg ggcatgcgag ctgattcggc aacagacggc
      421 gaaagatcag gacctggtca tttcaatccg ccaggagaag cacggctggt ggttgcggcg
      481 gttcctggag cagacaggca ttccacacag tttttcagag tacggcccgc tggagatcct
      541 ggccgaacgc tggaacgtcc acgtgccaga ttggctcact ccggagaaca tcgtcgagaa
      601 tcacttgttg gacctccaca ttcgacagca aacgaaggtc gagttcgagg atgcactcct
      661 gtcccatctg ctcgggaaga tattcaacac ggtcgcgatt gccgaggaac atattcccac
      721 cctggttcaa gtgctcacgc ggcccgaaac atctcacgcc ttcagccagc acccgattgt
      781 gcgaaagtgc ctagaggtga aatgtggatg gtgggcggag cagacggaga tcagctggat
      841 caaagacatc tgcatccggc tctcgcaaga tttagacacg atctggcagg aactaaccgc
      901 ttgggctctg ttgggaactt accccagcaa gctgttggag tttgtggtgc cgctgcatcg
      961 cgccaaccag ttgcgttcga taccagcagg gatactgaat gggatgccct ttcacgggaa
     1021 gacaaagcag caggctgaga ctcaaattga gttgtttctc gcggaaatcg gcaaatccat
     1081 caagaacagt gatgactttg agcggatcct ggacgcgact tcaggcctgc tgagcactga
     1141 gttccggcac atgaaggaga ttctggctgc agacgtcttc ccagtgaaac gagaagatgt
     1201 tgaggcggtg aggcgaaaat ttcaaagttg ccctgaagtg ggaacttcga gcctgctgca
     1261 acttgacagt ctcatcgcgc ctccccgacc ccaactgcct cagtcatccg atcagtgggg
     1321 agtggaagag tggatccgct gggcgatccg cgactatatg ccttaccggc attggcaaac
     1381 gaaagcgaag gtgttcgacg ctgaggtgga acagcaggcc ggtcacttca ctgattggta
     1441 cctccgccaa tattccacgc tgcaccagca ggcatcagtg agtctgaccc atgtgctcag
     1501 ttcgctgcgg gatcggctga gcgctgatgc gcttgccttg attgtcctgc tggatggtct
     1561 gcctgcaatc tactggcggc atctcagcga agccctccgc gtggccggcc tacaccaggt
     1621 ggattccggt tggcggctgg cgccgctccc aagccacact gctctctgca agcccctgct
     1681 gttgagcggc ggttgggatg actcgaacca ggattatcgg gcgattgttg agctgcgcgc
     1741 ccagcaagag tggggaaaac gagcaatcgt ctatgtttcg agccttaaag aactcagtga
     1801 ctgtcaactg tcccaggaac caagcgttgt aatctttaac tttctctcga cggatgaact
     1861 cctgcacgcc gatgtggaat cggaaaatgc cacccacgag gagaagctgc acctcctctt
     1921 cgtcaaattg caggagacgg tcgtcgatct ctacaaacgc tggggcggac caatcgacca
     1981 gttttccctt tatgccttga ccgaccacgg cgcgtgccgg gtattgggag aggagaaaca
     2041 gactttggac tccaaggttg tgcagaaaat attcaccgag gccaatcacc gctttgcgga
     2101 agttggtgcg gatgaggcgc agaacgtgcc tcagaatctc tggggcctcg ggtatcggtt
     2161 ccaacctcct attgccaagg tgcaagatcg ttgctttttc atccccaaag gccacaacac
     2221 cgtgaagctg cccaagcagg ccaaaggata cttgcatgga ggcgccaccc cggaagagat
     2281 catcgtccct tgggtggtgt ggcgtactgt gcgaccggcc atcaaagacc tggccgcccg
     2341 gttccttgat ctgccagttc ccattgtttt ctatgtccaa aggctgacca ccctggcggt
     2401 cgagatctca aatcctaata atcagagggt gcgagtcctg gagatccgtg tcgtgcagcc
     2461 ggaaacggat cttgtcaagt ttgaaccact agatctcgag ggaaagcaga gtggccagat
     2521 cgctgcttcc atgtatttca aaaagtcagc attgggaagg ggaattcttc agcttgcgtt
     2581 ggactatg
//
LOCUS       DAOQUC010000491         2584 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_48165_length_2584_cov_4.134045, whole genome shotgun sequence.
ACCESSION   DAOQUC010000491 DAOQUC010000000
VERSION     DAOQUC010000491.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2584
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48165_length_2584_cov_4.134045"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            433..1338
                     /locus_tag="PKH07_09160"
     CDS             433..1338
                     /locus_tag="PKH07_09160"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNT35152.1"
                     /translation="MGAFKCSVIIPTFRQTSLLEQTLTTLSRQSLARDEYELVVVDNN
                     TDPQYSTRVREAAEKFSAVSLHEPKPGAAAARNRGVDHGASEIVVFVDDDIEVREDFL
                     QQHLAIHQREEKAVVVGRVVEGLKTAGWFGRFLNDCQIINRQPDGATEVDFRQFYGAN
                     ASVRKRWIVEAGMFDPTFLRRQDGELAFRLKKMGLNFRSCPSAVAIHHSQFSPEGYLK
                     RARDNGYYLAMLWTKHPELKVLENEAQYRGLKPIVARVLGNCLFALGWLVYPITPLFV
                     HKGFVARVLVENVRGIRDFETRQEC"
     gene            complement(1390..>2584)
                     /locus_tag="PKH07_09165"
     CDS             complement(1390..>2584)
                     /locus_tag="PKH07_09165"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNT35153.1"
                     /translation="FGNLVHKYSDSESASRGGRIGPVSQGSMDPVIEREIFSLEPGQV
                     SSCIETYSGFHLFIVDNRTSALSPQWDQIKDRVISQIEEEHYGELVQKTKEETLKTAD
                     VTNNLDKLVVMNKPSDLLFRVGETRFTVDDYLYLYPHSRPEIGSGATGVERFDKNVLR
                     EVFERALLAEAARRRGYLDVSEIRKQLERKSRQLLIDLQLQSLCSEHFKDIVSDEAQL
                     RAFYDSEKETFRTDLRVIVDRVTLGPATAQGEESTQAQQLARKRLNKQVTEVFELLRN
                     GSSASELSKHGVQQWRSSESPQEITHFEQELQESLRTKAHQLLDSPPVKDVLPDPRVI
                     EPVKVEDKFVILRIREVFPSRLKAFEEVRHEIEGRLLETKQQDVALQQAGEILMAIQF
                     QLTRR"
BASE COUNT          589 a          650 c          707 g          638 t
ORIGIN      
        1 acaaagaggc tggacgcctc ttctcctttt gagatcgaaa gaagaatcag acagagcaag
       61 agccttgcca atctgttcat aaacgagaag aataaatcgg aaggtcagaa gatttacgga
      121 attccacgtc gtctccacct gtgtctctcc tttgcgttcc gtcaaacaaa caagtttccc
      181 agaaaagtca gatagccaag tgtctctacc tttacgacag aggatttggg gttgtcaagg
      241 agaatctcca agacgcaaaa gagttgtctt gccgctgagc gctgtgcacc gcaaagagcc
      301 ggtaggttct gtagagggga cattttggct gtgctaccac actttgcctt gacgccgttg
      361 acgatccgac tgcagcatga tagcttttcc aacagttctc attcactgag gttttcggaa
      421 ctggattgaa gcatgggtgc gttcaaatgc agcgttatca taccgacctt tcgtcagacg
      481 tccctgttgg agcagaccct gacaacactg tccaggcaga gccttgctcg ggatgagtat
      541 gaactcgttg tcgtggacaa caacaccgac ccgcagtaca gcacaagagt ccgcgaggcg
      601 gcggagaaat tctctgccgt ctcccttcat gaaccgaagc caggagcggc ggcggctcga
      661 aacaggggtg ttgaccatgg cgcgtcagaa attgtggttt ttgtggatga cgatattgag
      721 gttcgcgaag actttctgca gcaacatctg gcgattcatc agcgggagga aaaggcagtt
      781 gtcgttggca gagtcgtgga agggttgaag accgcaggtt ggtttggccg atttctcaat
      841 gactgccaga tcatcaatcg ccagcctgat ggtgcgactg aagttgactt tcgtcagttc
      901 tacggtgcca atgcctcagt gagaaaacga tggatcgttg aggcagggat gttcgatccg
      961 acgtttcttc gaagacagga cggagagctg gccttccggc tcaagaagat gggcctgaac
     1021 ttccgatcgt gtccgtccgc cgtcgccatt catcactcac aattttcgcc cgaaggctat
     1081 ctcaaacgcg cccgagacaa cgggtactat ctggcgatgc tctggacaaa gcatccggag
     1141 ttgaaggtct tggagaatga ggcgcaatat cgaggtctga agcctattgt cgctcgggtt
     1201 cttgggaatt gtctttttgc gttgggttgg cttgtttacc cgatcacacc attgtttgtt
     1261 cacaagggct ttgtcgcccg agttcttgtt gaaaacgtcc ggggaattcg agattttgaa
     1321 acgagacaag agtgttgatt ccccggtctc ttgcgacgcc tcttttcctt gccggagatt
     1381 cacctgaggt cagcgccggg tcagttggaa ctggatcgcc atgaggatct cacctgcctg
     1441 ctgcagtgcc acatcctgtt gtttcgtctc cagaaggcgt ccttcgatct cgtgtcggac
     1501 ttcctcaaag gccttcaggc gggaaggaaa cacttcgcgg atgcgaagaa tcacgaattt
     1561 gtcctcaact ttgaccggct ctatcactcg aggatcggga agtacgtctt tcacgggggg
     1621 cgagtcaagc agctgatgtg ccttggtgcg cagcgattcc tgaagttcct gctcgaagtg
     1681 agttatctcc tgaggggatt cactgctgcg ccattgctga acaccgtgct tgctgagttc
     1741 agaggccgaa gaaccgtttc gaagaagttc aaagacctct gtcacctgtt tgttcaatcg
     1801 cttacgtgcg agttgttgtg cctgagtaga ctcctccccc tgagccgttg ccggccccaa
     1861 agtgacccga tctacaatga cgcgaaggtc ggttcgaaag gtctcttttt cggaatcgta
     1921 gaacgctcgc agttgggctt catcgctgac aatatccttg aagtgctccg agcagaggct
     1981 ctgcaactga aggtctatga gaagttgtcg gctcttgcgc tcaagttgtt ttcgaatctc
     2041 ggaaacgtcg agatagccgc ggcgcctcgc tgcttcggcc aacaaagcgc gttcgaaaac
     2101 ctcacgaagc acgttcttgt cgaatcgttc aacgccagtg gctccagaac ctatctccgg
     2161 cctggagtgt ggatagagat agaggtaatc atcaacggta aaacgcgtct caccaacccg
     2221 aaacaacaaa tccgacggtt tgttcatcac aaccagtttg tccaggttgt tggtgacatc
     2281 ggctgtcttg agtgtctctt ccttggtctt ctgaacgagt tcgccgtagt gctcttcctc
     2341 gatctgggag atgactctgt ctttgatctg atcccactgg ggagagagcg cactggtgcg
     2401 attgtccacg atgaaaaggt gaaagccgga ataggtttcg atacaggaac tgacctgacc
     2461 cggttccaga gagaagattt cccgctcgat gactggatcc atggaccctt gagagaccgg
     2521 tccaatgcgg ccaccacggg atgcagactc agagtccgag tatttgtgaa ccagattgcc
     2581 gaac
//
LOCUS       DAOQUC010000492         2578 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_48357_length_2578_cov_3.104637, whole genome shotgun sequence.
ACCESSION   DAOQUC010000492 DAOQUC010000000
VERSION     DAOQUC010000492.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2578
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48357_length_2578_cov_3.104637"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..977)
                     /gene="larA"
                     /locus_tag="PKH07_09170"
     CDS             complement(<1..977)
                     /gene="larA"
                     /locus_tag="PKH07_09170"
                     /inference="COORDINATES: protein motif:HMM:NF033504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HNT35154.1"
                     /translation="MSEQRTVHVAYGKTTVPLSLSPDAEWEIIQPHYEAPLPNAQQVF
                     LDLCKKPVGCPTLGDIVQETDRVAVVTSDGTRAVPNHLLIPWLLEVLPCPVENVTVIL
                     GTGTHRPNTPQEIEAMVGSEVAKQVRIINHNCFDPSENCRVGQTSTGGETFLDRHYVE
                     ADKRIVLGFIEPHFFAGFSGGAKGIVPAIASRDTIFHMHSAPLIADPKSTWGILDENP
                     LRQEIEEMVGLCPPDFMINVTLNLKKEISAIFAGHYQTAHRPGCEHVRKVAMRKLLHR
                     FPIVITSNSGFPLDQNLYQTVKGLSAAARVVEQDGLIFVASECSDGVPN"
     gene            1149..>2578
                     /locus_tag="PKH07_09175"
     CDS             1149..>2578
                     /locus_tag="PKH07_09175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35155.1"
                     /translation="MKHLVSRHYCIQLALLVLCHFVSPLDAQSADWRFCYPLPQGNSL
                     HAVWSAGENDVFVGGDAGLVMRWNGSDWTIMNTPTQKSIYSISGTSATDVWAVGGDSK
                     TTDYTKIGLVMHYDGSKWSLVTPPDFSGYTYILRGLTALAPNNAWAVNNGGPSLIRWN
                     GTNWQFDLLNGAYFLEGRFETTFALDANHVFAVGTHGQIVHRYDNLWHTELKEETGSF
                     STNILTGIWGSDLDNLYACGNWGQVYKRNPNGTWSSMPSVFPSALGNGFLGMTGRSAS
                     DIYLLRTQSIVHYDGADTATEISFDYQIGSSWTAGTIAPDGLYLVGPSGTASKYRFAG
                     EQQTSGLSSLSASGPRVGLWNQNIRECGDGFLIYGYGQPPAYEPLLYFDGTIPVRFPS
                     VPDDFDSARETSVYAENINDITMTWWSSNTNFFYRKHWDGEKWSDAGYDYPFNMTHFF
                     KSPTGILYGMTNGNVFKKDKVLGWTR"
BASE COUNT          615 a          697 c          673 g          593 t
ORIGIN      
        1 tgatttggaa ccccgtccga gcattcactc gcgacaaaaa tgagcccgtc ctgttcaacc
       61 actcgagccg cggcagacaa acctttgacc gtctggtaga gattctgatc caaagggaaa
      121 ccgctgttgg aagtgatcac gatcggaaag cgatggagca actttcgcat cgccactttt
      181 ctgacgtgtt cacatccggg tctgtgggca gtctgatagt gccccgcaaa aatcgctgaa
      241 atctccttct tcagattcag cgtgacattg atcatgaaat ccggaggaca cagcccgacc
      301 atttcctcga tctcctgtcg cagcggattt tcgtccagaa ttccccaagt gctcttggga
      361 tcggcaatga gtggagcgct gtgcatgtgg aagatggtgt cacgactggc aatcgccgga
      421 acaattcctt tcgcaccacc agagaaaccc gcaaagaaat ggggctcgat aaagccgagc
      481 acgatccgct tgtctgcttc gacataatgc cggtcgagaa aggtttcgcc acccgtggat
      541 gtctgtccga cgcggcagtt ctccgagggg tcaaagcagt tgtgattgat aattcgcacc
      601 tgcttggcca cttcagaacc aaccatcgcc tcgatctcct gaggggtgtt tggccgatgt
      661 gtccctgttc ccagaataac ggtcacattc tcgacgggac acggcaggac ttcaagaagc
      721 caggggatca aaaggtgatt gggaacggcg cgagtaccat cggatgtgac aacagcgact
      781 cgatctgtct cctgaacaat atccccaagc gttgggcacc caacgggctt tttgcagaga
      841 tcgagaaaga cctgctgcgc gttgggcagc ggagcctcgt aatgaggctg tataatctcc
      901 cactcggcgt ctggagacag actcagggga accgtggtct tcccataggc aacgtggact
      961 gttcgttgtt cgctcatgtt ctacacctca tctcacgacg acgactctgg ataaactagc
     1021 ggacattcaa gccaatggca agtaacgacc tctgtagaag agccatgtgg aaaatggatt
     1081 tcgccaccac caaaatgtgc tagaatgacg ggaaatatgt ctcttccatg agaaaggaaa
     1141 tgactttgat gaaacatctt gtatctcgac actattgcat tcaactcgcg ctgcttgttc
     1201 tctgccactt cgtgtctccg ctggatgcgc agtctgccga ctggcgattc tgctatcccc
     1261 tcccacaagg caattcactt catgctgtct ggtctgcagg ggagaacgac gtcttcgttg
     1321 gtggagacgc cggtttggtg atgcgctgga acggctctga ctggacaatc atgaacacac
     1381 ccacccagaa gagcatctac agcatctccg gcacttcggc aactgatgtc tgggcagtgg
     1441 gtggcgacag caagacgaca gattatacca agatcggact tgtcatgcac tacgatggct
     1501 ccaagtggtc gttggtcact ccgccggact tctcgggcta cacctacatc ctcagaggac
     1561 tgactgcgtt ggcacccaac aacgcctggg cggtcaacaa cggtggccct tccttgattc
     1621 gttggaacgg tacgaactgg cagtttgatc tcctcaatgg agcctatttc ctcgaaggca
     1681 gatttgagac aaccttcgct ctggacgcga accatgtttt cgctgtggga acacacggtc
     1741 agatcgttca tcgctacgac aacctctggc atactgaact gaaggaagag acagggagtt
     1801 tctcaaccaa catcctcact ggaatctggg gctccgatct ggacaacctc tatgcctgtg
     1861 gaaactgggg acaggtctac aagagaaacc ccaatggcac ctggagcagc atgccctctg
     1921 tctttcccag tgctctgggt aatggatttc tgggcatgac tggccgctcg gcgtcagata
     1981 tttacctgct ccgaacgcag tcaattgtcc attatgacgg cgcagacacc gccacggaga
     2041 tcagtttcga ctatcagatc ggaagttcct ggaccgcagg cacaatcgca ccggacggcc
     2101 tctatcttgt cggaccgtct ggaacggcca gcaaataccg atttgccggc gaacaacaga
     2161 catcgggcct ttcgagtctt tcggcaagcg gtccgcgcgt cgggctctgg aaccagaata
     2221 tcagggaatg tggggacggc tttctcatct atggctacgg tcagccacct gcctatgaac
     2281 cactgctcta tttcgatgga acgattccag tgcgctttcc gtcggtgcct gatgattttg
     2341 actctgccag agaaacatcg gtctacgccg agaacatcaa tgacattacg atgacatggt
     2401 ggtcgtcgaa tacgaatttc ttctaccgaa aacactggga tggtgagaag tggtctgatg
     2461 cgggttacga ctatccgttc aacatgacgc actttttcaa atcaccgaca ggcatcctgt
     2521 atggtatgac caacggaaac gtgttcaaga aagacaaggt tcttggctgg actcgaat
//
LOCUS       DAOQUC010000493         2575 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_48457_length_2575_cov_2.862698, whole genome shotgun sequence.
ACCESSION   DAOQUC010000493 DAOQUC010000000
VERSION     DAOQUC010000493.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2575
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48457_length_2575_cov_2.862698"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..870)
                     /locus_tag="PKH07_09180"
     CDS             complement(<1..870)
                     /locus_tag="PKH07_09180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HNT35156.1"
                     /translation="MRVALCAPWMTFVIVAFFSALLSASCDASQRVPARVEVLSDGRL
                     AVNGVPTFFLGFAPGPPMDLKTPEGGNGWQELAEGGISVVRQGAAPRTDWNDEEESRF
                     EQYLDNAIKHGVRVWPMLRELVVVSDTQSRERLTSFVNRFKDHPGILLWKSADEPQWG
                     QIPAEPLTEAYRLMRRLDPNHPVWICHAPRGTLDELRPYNDACDVLSTDIYPVSEPPG
                     KHSLEANKGLSMVGDYTKRMVELSEGRKFVFMVLQVCWSGVMSRPEKTNRLMFPTFRQ
                     ERYMMYQAIICGAR"
     gene            complement(1033..2356)
                     /locus_tag="PKH07_09185"
                     /pseudo
     CDS             complement(1033..2356)
                     /locus_tag="PKH07_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007651515.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
     gene            complement(2368..2547)
                     /locus_tag="PKH07_09190"
     CDS             complement(2368..2547)
                     /locus_tag="PKH07_09190"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNT35157.1"
                     /translation="MDKIFVPFFTTKDVGQGTGLGLPVVHGIVTAHGGSIEVSSHVGE
                     GTRFVVHLPLSFTDV"
BASE COUNT          595 a          763 c          625 g          592 t
ORIGIN      
        1 tctggctcca cagatgatgg cttgatacat catgtagcgt tcctgccgaa acgtcgggaa
       61 catcaggcga ttggtctttt cgggacgact catcacacca ctccaacaga cctgaagaac
      121 catgaaaacg aacttcctgc cttcggacaa ctcgaccatt cgcttggtgt aatcaccaac
      181 catgctcagc cccttgttgg cttccagcga atgtttccca ggtggctcac tcacagggta
      241 gatatcagtg ctcagcacgt cgcaggcgtc attgtagggt ctaagttcgt cgagagtgcc
      301 tcgtggagca tggcagatcc acacggggtg attcggatca agtcggcgca tgagtcggta
      361 cgcttcggtg agtggttccg ccggaatctg tccccattgc ggctcatcgg cacttttcca
      421 aagcaggatc cccggatgat ccttgaagcg attgacaaac gatgtcagtc tttccctgct
      481 ctgtgtgtcg gaaacaacaa cgagttctcg aagcatgggc cacacgcgca ccccatgctt
      541 gattgcgttg tcgagatatt gctcgaaacg gctttcctct tcatcgttcc aatccgtcct
      601 gggcgccgcg ccttgcctca cgacagagat tcccccttct gccaactcct gccagccatt
      661 gcccccttca ggcgtcttca gatccatcgg cggtccaggc gcaaaaccaa gaaagaatgt
      721 cggcacacca ttcacagcca gccgaccatc ggacagaact tcgacgcgag cggggacacg
      781 ttgcgatgca tcgcatgaag cgctcaagag tgcggagaag aaagccacta tcacaaaggt
      841 catccacggt gcgcaaagtg caactctcat cttatgaact ccaacaaaaa tgccagcagc
      901 tgtccctgtg ttacaattct ctgtcttgag tctcttgttc cttcgcatcc gacatggaat
      961 gcctgctcat ccggattgcc aagtccattt ccaggaatag atcccaccgt tcgatcggtt
     1021 cgaactttac tctcaacgga tctttgacct cagtgtcttt ctatccaccc caagtatgct
     1081 tgccgcgcgg ctgatattgc tgtcaacgct ggcaagcaca ttccgaatat gctccgcttc
     1141 gacttctgca agggttctgt gcactcccag agatttggga acagagaacc tcatgagaga
     1201 cggaagatcg gatacctcga ctgttgtgcc ttccatcatc acaacaaggt gctgaatcag
     1261 attctccaac tctctgacat tgccaggcca ttcatagtgt cgcatggcat caagcgcttg
     1321 tgccgagaac tcaggaggct gtttttcgta atctgtcgca tacttggcag caaagtgcct
     1381 ggcgagaacc acgccgtcct ctcctctgtc tcgaagtgcg ggaactgaga tggcaatcac
     1441 attcaaccga tagaaaagat cttcccgaaa cttgccccgc ttgacgagcg agagaaggtc
     1501 atggtttgtg gcggcaacca cccggacatc cactctgcgt gccttgctgg aaccgaccat
     1561 gcggacttcc ttgtcttgga gaacccgaag aagactaacc tgcattgaag gactcgtctc
     1621 gctgatttca tccagaaaga tcgttcctcc gtcagcagaa tggaagaatc cggcacgaga
     1681 ttccgctgca ccggtaaagg ctccctttac atgaccgaac agttcactct cgagaagccc
     1741 ttctgggatg cttccgcagt tcaccggcac aaagggggcg gaagccctcg cgccgctgta
     1801 gtgaatggca cgagcaacca attccttccc tgtaccgctc tccccagtga tcagaaccgt
     1861 ggcagaggtg gctgaagcct tctcgatagc agcaaacact tttcccatcg cagcggccaa
     1921 tgatcccgta ctgtgccatg gacacatccg aaggagactg gcttgaaact cgcttggcct
     1981 gcaatttgtc cagtactttc cgaaccgctg tcaggagttc ctcatcggtg aagggttttg
     2041 ccagatattg ctcggcaccc gccttcaccg cttcaaccgc cccatccaga gacggatacc
     2101 cagtgatcat catgatctct gtttctctgt ggttgtgacg aacgtgtcgg acgagatcca
     2161 gcccgctgat gccgggcatc ttcagatcgg tgatcaccag gtcgtacgaa gcctgctcaa
     2221 ggagtgtgac ggcctgctga acatcggaac acgtgagcac ttcatatccc gcggcgacca
     2281 gattgcgttg cagtacctca agggtgctga gcgaatcatc taccgcaagg atcctctctt
     2341 tgtgcttctc tgtcacactg acactcctca aacatctgtg aatgacaacg gaagatgaac
     2401 aacaaagcgc gtcccctcac caacatggct cgaaacttca atggaacccc catgtgcagt
     2461 gacgatccca tgcacaaccg gaagtcccag accggtgcct tgacctacgt ctttggtcgt
     2521 gaagaacggg acaaatatct tgtccatgat atctggctcc atgccacatc cggtg
//
LOCUS       DAOQUC010000494         2574 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_48483_length_2574_cov_3.308059, whole genome shotgun sequence.
ACCESSION   DAOQUC010000494 DAOQUC010000000
VERSION     DAOQUC010000494.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2574
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48483_length_2574_cov_3.308059"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            917..>2574
                     /locus_tag="PKH07_09195"
     CDS             917..>2574
                     /locus_tag="PKH07_09195"
                     /inference="COORDINATES: protein motif:HMM:NF019314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNT35158.1"
                     /translation="MIYLDLVLNLTLLVALSVISGFVDKRWPRHTRLGVLMQGSLFGV
                     TSVIGMLKPLVVGPGLIFDGRSVLLSLCALFFGPWAAVTAAPMPIACRIWMGGSGSRM
                     GVLVVLSSVAIGLWAHSRLRPHERPISSRSLYLFGWAVHLAMVAMMFTLPPEMIFQVM
                     FRIGPPVLLLYPLATLLAGKILADQLETQRLIRQLHESQEFTKTILDNLPIGVAVNSL
                     HPEVVFTYMNDNFPRFYRTSRETLAHPDAFWSAVYEDPDFREQLRKRVLEDCSSNDPQ
                     RMEWVDIPITRAGSETTYITARDIPVPGKSLVISAVWDVTDRRMAELALRESESRFRL
                     FAELAPVGIVISDVQENTLYVSQKFVEMFGYTMEDMPSVEHWWPLAYPDEKLRNQVRA
                     HWKQALEYARATRSEIAPVEFPVTCKDGTVREIEFRMSNSGDLNFVLFTDITERRRTE
                     RETEQLQSQLLQAQKMESVGRLAGGVAHDFNNMLMVIAGHTQMALEHLNPQDAVYADL
                     VEVQKAASRSTDLTRQLLAFARKQAIAPRVLDLNETVEGMLKVLR"
BASE COUNT          580 a          652 c          693 g          649 t
ORIGIN      
        1 gagcaagggt tcgcaacgtg cgacatgaga atcccccctc aatcccccct taacaagagg
       61 ggcaggggag atttgtccat aatgaggagg tcgtgactgt aacaaaactg aggttgagcc
      121 agcccctgcc tggctccttg acttctcttc gcagatattg tctctcattt ttaacactcc
      181 ctctcggcca gattcagccg gatactggtt aggaattgta tcccaaaatc cagccaggtg
      241 ctactactaa cgaatgagaa tggactttgg tcgcgttcgg tgcagaaagg actgaaccga
      301 aacacttcgt atctgtcttg tcctaagggg ctctcgaagg tgggagaaag gtgctctggg
      361 ttcaagaaat ccgcctgact tggtgcgcca gacaaggttc tgtctcggat ccaaaggctc
      421 cctctcatcc aacggaagcc gcagttgaag acctgatcat cctttcaact cctttccaat
      481 aaccatttta ccgcagagtc tccaggtgtc aaatgtttcg taggccggtc tgcattggct
      541 cagttatgca ggtgatacag ataggatatg ctctcccgaa aatcccccct gccttgttaa
      601 ggggggttca gaacaagaca ccccttcgtc aaggtggttc tgaaaacacc cttcgacaag
      661 gggggatcaa ggggggattt gtcatcccct tcgggtgcgc ttggccatga gaggcttcgg
      721 cagtgtcgca gagcctctgc aaccgaagca tacctctacc ccctccgtaa ctgagccagt
      781 aggccggttt tggtttgaca agggttccag attggataaa cttaagaaaa agggtacttt
      841 gttctgagtt cataagagtc ttgcattctg tacaagacgt agcgcgggtg gtttgtttct
      901 caaaggactg tgaaacatga tctacctcga cctggttttg aacctcacgc tgctggtagc
      961 actcagtgtc atctccggtt ttgttgacaa gcgctggcct cgacacaccc gactgggtgt
     1021 cctgatgcag ggatcgctct ttggcgtgac ctctgtcatc ggcatgttga aaccacttgt
     1081 cgttggtcct ggcctgatct tcgacggtcg ttcggtgctg ttgagtcttt gcgctttgtt
     1141 tttcggacct tgggcagcgg tgacggctgc ccccatgccg atcgcatgtc gcatttggat
     1201 gggtggttcc ggatcgcgca tgggggtttt ggtcgttctc tcgtccgtag cgatcggttt
     1261 gtgggcgcat tcaagactcc ggccgcatga gagaccgatc tcctctcgaa gtctgtatct
     1321 gtttggctgg gcggttcacc tggcgatggt cgccatgatg tttacgcttc ccccggagat
     1381 gatcttccag gtcatgtttc gaatcggtcc gcccgtgctt ctactctatc cactcgcgac
     1441 gcttcttgcg ggtaagattc ttgccgatca actcgaaacg caaagattga tcaggcaact
     1501 gcatgagagc caagaattta ccaagacaat tcttgacaat cttccaattg gtgttgctgt
     1561 caactccttg catcctgaag ttgttttcac gtacatgaat gacaactttc caagattcta
     1621 ccgtacctct cgtgagacac ttgcccatcc ggatgctttc tggagcgctg tgtatgaaga
     1681 tccggacttc agagaacaac tgcgcaagag agtcttggaa gactgctcaa gcaatgatcc
     1741 tcagaggatg gaatgggtgg acattcccat cacaagagca ggttccgaga caacctacat
     1801 caccgcgcga gatattccag ttccgggaaa gtccttggtt atctccgccg tctgggatgt
     1861 gacagacaga agaatggcgg agttggcgct gagagagagt gaatcgcgtt ttcgtttgtt
     1921 cgcggaactg gcacctgttg gcatcgtcat ttccgatgta caagaaaaca cactctacgt
     1981 gagccagaag ttcgtcgaaa tgttcggcta taccatggag gatatgcctt ctgtcgagca
     2041 ttggtggcct cttgcctatc ccgacgaaaa actcagaaat caggttcgcg cgcattggaa
     2101 gcaggcgctt gaatatgcgc gggcgacccg aagtgagatt gctccagtgg agtttccggt
     2161 gacctgcaaa gacgggacag tgcgagagat cgagtttcgg atgtccaaca gtggtgatct
     2221 gaatttcgtc ctctttacag atattaccga acggagacgc acagaaaggg aaacagagca
     2281 gctgcaatca cagctgctcc aagcgcagaa gatggagtct gtgggcaggt tggcaggtgg
     2341 cgtggcacac gatttcaaca atatgctcat ggtcattgca ggtcacactc agatggcatt
     2401 ggaacacctg aatcctcaag atgccgtcta cgctgatctc gtcgaggttc agaaggcggc
     2461 cagtcgttcg acagatttga ctcgccagct gctagccttc gcccgcaagc aggcgatcgc
     2521 tccccgcgtt cttgacttga acgagacggt agaaggaatg ctgaaagtat tgcg
//
LOCUS       DAOQUC010000495         2571 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_48569_length_2571_cov_3.352544, whole genome shotgun sequence.
ACCESSION   DAOQUC010000495 DAOQUC010000000
VERSION     DAOQUC010000495.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2571
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48569_length_2571_cov_3.352544"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1564)
                     /locus_tag="PKH07_09200"
     CDS             complement(<1..1564)
                     /locus_tag="PKH07_09200"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF037667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HNT35159.1"
                     /translation="MLGHGLLVRCLLLVVSLCLVPIYSHAIPEFLAATGTQVVLDISV
                     DAKGVSQVVSGANVDMAYKGVSAIVVFDLAAQQIEKPYALRVDAPEPPFLTVNGKELV
                     VVPVRGVDGWYYRVGVYYLAVGRNEIAMAPADDSSLDLAAVQMASLEHTAEDADFDRF
                     FSVQKPSLAVQPAIDPEQAKYDAAHYDLTITLNMGSRFVSGALMMTATSTNSTLTTAV
                     LDLDDNGGSIVVSQVDQGPSTATLPFTHNASSNRVFITLPAAVPAGSTFTVRVFYSGT
                     PLTTGTFGPGYNRTTHSGTSLIYTFSEPYKARTWWPCKDVPGDKATMDLHVTCPTGYF
                     VVSNGTLVSTVDNGNGTLTFNWSEAYPMATYLASICCTNYQKASGTYTALDGVTTMEV
                     AHYVYPESYAFDSQGLPGTLQAMGYFANTFGEYPFLTEKYVTATHGSGSGMEHQTCTS
                     MPNNDVGISGGTGRRNIHELSHMWFGDMITMAHFDHLWLNEGYATYCEALFYEWRDGR
                     QAYHNYVNAWTTA"
     gene            2089..>2571
                     /locus_tag="PKH07_09205"
     CDS             2089..>2571
                     /locus_tag="PKH07_09205"
                     /inference="COORDINATES: protein motif:HMM:NF037817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5610 domain-containing protein"
                     /protein_id="HNT35160.1"
                     /translation="MNAAAIGYSNDRAIQSYSLSGRLEYQSYEYAKVSRTNGASGLTD
                     IVELGRGEKVSIEFTLSMVQERTQSKIAARLPEAPSTPGLPSFQGLDTSPEATAQRIV
                     DFATAFLGAYKEQHPELGDVEALEEFMELIRGAIDEGFAQAKDIIDSLNALTPELNQT
                     I"
BASE COUNT          650 a          701 c          670 g          550 t
ORIGIN      
        1 cggcagttgt ccatgcgttg acatagttgt gatatgcctg gcgtccatcg cgccattcgt
       61 aaaagagcgc ttcacagtaa gtggcgtaac cctcattgag ccagagatga tcaaagtgcg
      121 ccatggtaat catatcgccg aaccacatgt gcgacagttc atggatgttt ctgcgaccgg
      181 ttccgccgct gatacccaca tcgttgttgg gcatgctggt gcaggtctga tgctccatac
      241 cggagccgct gccatgcgtg gcggtcacat acttctctgt cagaaatgga tactcgccaa
      301 aagtgttcgc aaagtaaccc atcgcctgaa gtgttccggg aagcccttgg gaatcgaagg
      361 cgtaactctc cggatagacg tagtgagcga cttccatcgt cgtgacacca tcgagagccg
      421 tgtatgtacc cgatgccttc tggtagttgg tgcagcagat ggatgccaga taggtcgcca
      481 tcggataggc ttcgctccag ttaaacgtca gagtgccatt gccattgtct accgtactga
      541 caagggtgcc attggacacg acgaaatagc ctgtcgggca ggtcacatgc aaatccatgg
      601 ttgccttgtc gcccggaaca tccttgcacg gccaccaggt ccgtgccttg tacggttcgc
      661 tgaacgtgta aatcaaagat gttccggaat gtgttgtcct gttgtatcca ggtccaaatg
      721 taccggttgt caggggagtt cctgagtaga acacgcgaac agtgaacgtg gagcctgcgg
      781 gtacggcggc gggaagtgtg atgaaaaccc tgttgctgga ggcgttgtgc gtgaacggaa
      841 gagtcgctgt ggacgggcct tgatccacct ggctgacgac aatactgcca ccattgtcat
      901 ccagatctaa aactgcagtc gtcaacgtgc tgttggtgct cgttgcagtc atcatcaatg
      961 caccggaaac aaaccgactg cccatgttca gggtgatggt caaatcgtaa tgagcggcgt
     1021 cgtacttggc ttgctccgga tcgattgcgg gctgaacggc caatgacggc ttctgaacgc
     1081 tgaaaaaccg gtcaaagtct gcatcctctg ccgtatgctc caaggaagcc atctggacag
     1141 cagccagatc cagtgaagag tcatccgccg gagccatcgc tatctcattg cgaccgacgg
     1201 ccagatagta cactccaaca cggtagtacc aaccatcaac ccctcgaaca ggaaccacga
     1261 ccagttcctt gccgtttacc gtcaggaacg ggggctctgg agcatctact cgaagggcat
     1321 agggcttctc aatctgttgg gcagcaagat caaagacaac aattgcggag acacccttgt
     1381 aagccatatc aacattggca ccagacacaa cctgggatac gcccttcgca tcaaccgaga
     1441 tatcaagaac gacttgcgtc ccagtggcgg cgagaaactc cggaatagcg tgggagtaaa
     1501 tgggaacgag acacagagag acaaccagaa gcaaacatcg caccagcaga ccgtgaccga
     1561 gcattggcaa ctccttaagc aaacgacgca tttgaatacc ctatgtgatg acgttacctc
     1621 actctcgcca tggcgggcga gaacacctaa acacccctcc ctccagagga aagggtgagt
     1681 gtaaccaaac agtccttctg ccacacaacc atacccagac ttctgcagat tctgcccgag
     1741 ggctgatgct aacctgctca gcttatctgt gggattctag tccagacaac tggcagtcgt
     1801 caacttgatc agccaatctc ccccatggga cttcacatca gtccgattgt ggtcgtcaga
     1861 catccgttcc tgaccgtcgc tcagagtcaa cagaaacacg agggcttcat agagagccct
     1921 tcataagatg ataacgacga atcaagagag ccgtgttcag aagtcgagtt ctgacaaaac
     1981 atttctcttg cagggattcc agcacatttc ctctcaagtt ccggtcgaat acggtcgaaa
     2041 accaaactag cgtgtgaaca atgtaatcct cttggggagg tcttgaaaat gaatgcagcg
     2101 gcgataggtt actcaaacga cagagcgatt cagtcctaca gtctcagcgg gcgacttgaa
     2161 tatcagtcct acgaatatgc caaggtgagc cgaaccaacg gcgcttcggg tttgacagac
     2221 atcgtcgaac tcggacgagg ggaaaaggtg agcattgagt tcactctctc catggttcag
     2281 gaacgaaccc agagcaaaat cgcagcacga cttccagaag ccccatccac acccgggctt
     2341 ccttcgtttc agggtctgga caccagcccc gaggccaccg cgcagagaat tgtggacttt
     2401 gccaccgcct ttctcggtgc ctacaaagaa caacatcctg agctgggaga cgtcgaggcc
     2461 ttggaggaat tcatggagct gattcgtggc gccattgatg agggttttgc ccaggccaaa
     2521 gacatcattg attctctgaa tgcgctcacc cctgagctca accagaccat c
//
LOCUS       DAOQUC010000496         2566 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_48726_length_2566_cov_2.675030, whole genome shotgun sequence.
ACCESSION   DAOQUC010000496 DAOQUC010000000
VERSION     DAOQUC010000496.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2566
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48726_length_2566_cov_2.675030"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1125
                     /locus_tag="PKH07_09210"
     CDS             <1..1125
                     /locus_tag="PKH07_09210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35161.1"
                     /translation="PTRTPSETPSYTPTITPTNTPSETPTNTPTVTPSNTPTPTVTNT
                     PTPTYYLGLYILSGLGTIHAVDDAPVFDPMGQFMGWDIARDIEVTWDQNGHASGLYQL
                     SGFGDVHQFGNAPIYPDSPIPYAGWDVFRDLEPAMDWTDERYGQAGFYLLDAFGGVHP
                     IGETFRTDPGSGRQYFKQYRTARTLEFGEDRYDYWGWDIARDMEVAVGLRQDDPQSQP
                     YPIVRGYYILDGYGAVHWSLEGDSGNVELAPWFPAGQPYFGWDIAKDIELSPTGLGYY
                     LLDGYGGIHIVGDARLPDAPPSLYYGWDIAKSLKIFVDKNGTTRAIVVLDGFGGIQDW
                     GTLELGPSQFFGWDIARAAEGLPFHNYEFCDVHLSAFPTE"
     gene            complement(1212..2369)
                     /locus_tag="PKH07_09215"
     CDS             complement(1212..2369)
                     /locus_tag="PKH07_09215"
                     /inference="COORDINATES: protein motif:HMM:NF025465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PmoA family protein"
                     /protein_id="HNT35162.1"
                     /translation="MAVQLVVKAGAHARRNCVTRAVVPWKGSLPKSVSMKSASETVVC
                     AQVESQTEDSLSVVWVVPAMLAGETETWTLHVADEKTPEEVVCAQETDDQLSLKVTGT
                     EVGRYQYGSKWARPFLYPLLAPKGLRPTRGFPVVEGIADEDHDHPHHKSVWVAHGDVN
                     GTDNWSEHTGHGATRHLGFNKIISGSVFGGFSSSNEWLTGDEKKILSESREVLLYNTS
                     NDERLLDFAITFHATEGDVKFGDTKEGGLLSVRVRGSMNGKREGLITNANGALTESET
                     WGHRAAWCDYSGPADGLLCGIAVFDNEANPLFPTYWHVRDYGLMTANPFALSAYKNDK
                     TLDGSWTLPAGKTATFRYRILIHLGNCSEGRVAEKYQDYANAPRVDVVSGA"
BASE COUNT          586 a          727 c          664 g          589 t
ORIGIN      
        1 ccgactcgga caccctccga gaccccatcg tacacaccga cgatcactcc aaccaacacg
       61 ccatcggaga ccccaacgaa tacgcccaca gtgacaccga gtaacacgcc gacaccaaca
      121 gtcaccaata ccccaacgcc gacatactat ctggggctct atattctcag cggtttggga
      181 acgattcatg cagtagatga cgcgcccgtt ttcgatccaa tgggacagtt catgggatgg
      241 gatatcgccc gagacatcga agtgacctgg gatcaaaacg gtcacgccag tggtctctat
      301 cagctgagcg gattcggtga tgtgcatcaa ttcggaaatg ctcccatcta tccggactca
      361 cccattccat acgctggctg ggacgtcttc cgagatcttg aacctgctat ggactggacg
      421 gatgaacggt acggacaggc aggtttctat ctgctggatg cctttggcgg cgttcatcca
      481 attggcgaaa cgttccgaac agatcccggc agtggtcgcc agtatttcaa gcagtatcga
      541 accgctcgaa cgcttgagtt cggggaagac cgctacgatt actggggctg ggatattgcg
      601 cgcgatatgg aagttgctgt tgggctgcga caggacgatc cgcagtcaca gccctatccg
      661 atcgtccgtg gttactacat tcttgacggc tacggtgcag ttcactggag cctcgagggt
      721 gacagcggca acgttgaact ggcaccttgg ttccccgctg gacaaccgta ttttggatgg
      781 gacatcgcca aggacattga actgtcaccg actggcttgg ggtattacct gctggacggt
      841 tacggtggca ttcacatcgt gggagatgcg agacttcctg acgcaccacc ctcgctctat
      901 tacggttggg acattgccaa gtcgctcaag attttcgtgg acaagaacgg caccacacgg
      961 gcgatcgtcg tcctggacgg atttggcggc attcaggact ggggcactct cgaactcggc
     1021 ccgagccagt tcttcggctg ggatatcgca cgagcagcgg aaggccttcc cttccacaac
     1081 tatgagttct gcgacgttca cctgagcgca tttccaacag aataactgac gagaatcaga
     1141 tcaacagaac aggcgcatgg gattcctact catgcgcctg ctcttttgtt gaagaatcgc
     1201 tgaggctcct gtcaggctcc ggacacaacg tccactcgcg gagcattggc atagtcctgg
     1261 tacttttccg ccactctgcc ttcggaacag tttcccagat gaatgagaat ccggtaccgg
     1321 aaggtcgcgg tctttccagc tggaagcgtc caggaaccgt ccagagtctt gtcgttcttg
     1381 taagcagaca gggcaaatgg attggccgtc atcaggccgt agtcccgaac atgccaatac
     1441 gttggaaaga gcggatttgc ttcattgtca aagacggcga ttccacacag aagcccatcg
     1501 gcaggacccg agtaatcgca ccaggcagcc cgatgtcccc aggtttccga ttcggtcaga
     1561 gcaccgtttg cgttagtgat cagaccctcg cgtttcccgt tcatactgcc tcgaactcga
     1621 accgagagaa gacccccttc cttggtgtcg ccaaacttca catcgccttc ggttgcatgg
     1681 aatgtgatgg cgaaatccaa caggcgctcg tcattggatg tgttgtacaa gagcacttct
     1741 cgcgattcac tgaggatctt cttctcgtcg ccggtcagcc attcgttact tgaggaaaaa
     1801 ccaccgaaga ccgaaccgga gatgatcttg ttaaagccga gatgtcgggt ggcgccgtgt
     1861 ccggtgtgtt ccgaccagtt gtctgttcca ttgacatcac catgggcaac ccagacagat
     1921 ttgtggtggg gatgatcgtg gtcttcgtca gcaattccct ccacaaccgg aaatccacgc
     1981 gtcggtcgaa gtccctttgg agccagaaga ggatacagga acggacgtgc ccatttggag
     2041 ccgtactgat atcgtcccac ttctgtgcct gtcaccttga ggctcaactg gtcatcggtt
     2101 tcctgggcgc agacgacttc ctccggagtt ttctcatcag ccacatgaag cgtccatgtt
     2161 tctgtctctc cagcgagcat cgccggcacc acccaaacca ctgagagcga atcttcggtc
     2221 tggctctcaa cttgtgcaca gacgacagtt tctgaagcgg atttcatgga taccgatttc
     2281 ggcaacgaac ctttccaagg cacaactgcg cgtgtgacac aattgcgacg agcatgagcc
     2341 cctgctttga caaccaactg cactgccatt tcaatacctc ctgcattctg agttccaaaa
     2401 cagtccgatt ctgcatgaaa atccctcgtc tgtgcaaatg cagaacgacc gcccaatgaa
     2461 atcatttcga tttgagaaga atccacattt gggctactct ctctcatgtg tgcgagatga
     2521 aaagcgcagg ttaaccggtt tttggaatcg gtcaatgtct atgcgc
//
LOCUS       DAOQUC010000497         2561 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_48868_length_2561_cov_3.007981, whole genome shotgun sequence.
ACCESSION   DAOQUC010000497 DAOQUC010000000
VERSION     DAOQUC010000497.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2561
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48868_length_2561_cov_3.007981"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..400)
                     /locus_tag="PKH07_09220"
     CDS             complement(<1..400)
                     /locus_tag="PKH07_09220"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HNT35163.1"
                     /translation="MNRYRFVVILLILTSCAIDASAFGFGKVKEYCNPLDVPVADPQI
                     LKDGDTYYVYGTRHGGRGYEAFSSKDLVHWRREGFCFDKNRDSWGQWDFWAPEVIRSK
                     DQYYLFYSAYSPVEKRHSVCVAVSDSPKGPF"
     gene            710..1897
                     /locus_tag="PKH07_09225"
     CDS             710..1897
                     /locus_tag="PKH07_09225"
                     /inference="COORDINATES: protein motif:HMM:NF022623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HNT35164.1"
                     /translation="MELFQSGNGRQSRWVSFENPTGARGAAAQENKGAKGHAFDHLEQ
                     GETKTLLDVQGSGMVRRMWFTIGPRNPQILRGLRLEMWWDGAATPAVTVPFGDFFGFL
                     MGAVPPPFENALFASPEAKSYVCYIPMPFRSAARITLTNDSPDRLAHLFYDIELTMDE
                     QHGDDVLYFHSSWRRERMTKCGKDFQILPKVKGVGRFVGSHMGMMTNPILKGWWGEGE
                     IKIYLDGDREFPSLVNTGTEDYISTGWGQGVFINRYHGSFVADGDKKRWTFYRYHVPD
                     PVFFYEDCVVTMQQIGGTMKAEVAQMMREGKPIQPISLDSSDHREFYKFLEMQNVNLE
                     DDSLPDGWVNFYREDDWCATAFFYLNKPENGLPALADYAERVAYISDKPEGAPQSNEA
                     INV"
     gene            complement(1973..>2561)
                     /gene="lnt"
                     /locus_tag="PKH07_09230"
     CDS             complement(1973..>2561)
                     /gene="lnt"
                     /locus_tag="PKH07_09230"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HNT35165.1"
                     /translation="LLGSDGAPLGEHFKHKLVPFGEYIPFSNVFPVLKRVFQNANPNL
                     NTQPGREFQIWTLKDVRMAAQVCFEILYPNLARRFVREGARLIINSANDGWYQDIGQT
                     EQHLALGIYRCIETRVPMVRATNTGVSAYVSAAGEIQGETSPFEQPWVDCRSVPVPEL
                     SSFYRRFGDVFGIGCVLFVILAFVRNAIISRNRHL"
BASE COUNT          629 a          672 c          691 g          569 t
ORIGIN      
        1 caaagggacc tttaggtgag tccgagactg ccacgcagac ggagtgcctc ttttcgacgg
       61 gggaataagc actgtagaac aaatagtact gatccttgga acggatgacc tccggtgccc
      121 agaaatccca ctgtccccat gagtcacgat tcttgtcaaa gcagaaaccc tctcgtcgcc
      181 aatggacaag atctttggac gaaaaggctt catagcctct tccgccgtga cgggttccat
      241 agacatagta cgtgtcacca tccttgagaa tctggggatc agcaacagga acatccagcg
      301 gattgcagta ctctttgact ttgccgaacc caaatgctga agcatctatg gcacacgacg
      361 tcagaatgag cagaatgact acaaaacgat accgattcat gaaatgacct ccctgtgtcg
      421 tatctcagaa cacctctgcg ctgatcagaa aatgccacaa tctggaatgt tttccaatca
      481 aaaaccatcc tgctgcggat cggtgatttg acatatcggc ttctccgcat ctaccatcac
      541 aagaccgtaa gagatcaatg gacagcggat ctgaagcccc tgccgataga agcatccgca
      601 ggttttctct gagggggggt gtcggaatgt ccaatcctca gagaaacagg aaaatccgca
      661 agtctccgtt gaatgtcatt caagacagag actgtttgag gagttgttca tggaactgtt
      721 tcagagtggt aacggcagac aatcgcgctg ggtgagtttt gagaatccca caggtgccag
      781 gggtgcagcg gcacaggaaa acaagggagc caaggggcat gccttcgatc atctggagca
      841 gggagagacc aaaacgttgc tggacgttca ggggagtggc atggttcggc ggatgtggtt
      901 taccatcgga ccgagaaatc cccagattct gcgcgggctg cgtctcgaga tgtggtggga
      961 tggtgcagca actcctgcgg tcactgtgcc gtttggtgat tttttcggct tcctcatggg
     1021 cgccgtgcca ccgccttttg agaatgcact gttcgcttct cctgaggcga aatcatacgt
     1081 ctgttacatc ccgatgccgt ttcggagcgc cgcccgcatc acgctcacca acgactcccc
     1141 cgaccggctg gcgcatctgt tctatgatat cgagttgacg atggacgaac agcacggcga
     1201 tgacgtcctt tactttcact ccagctggcg acgcgagcgg atgacaaaat gcggtaagga
     1261 tttccagatt ctgcccaagg tcaagggggt cggacgcttt gtgggttccc acatgggaat
     1321 gatgaccaac ccgattctca aaggatggtg gggcgaagga gagatcaaaa tctacctcga
     1381 cggcgaccgc gaatttccgt cactggtaaa cacaggtacc gaggattaca tcagcactgg
     1441 ttgggggcag ggggtgttca tcaaccgcta ccatggctcg ttcgttgcgg atggtgacaa
     1501 gaagcgctgg accttctatc gctaccatgt tcccgatccg gttttcttct acgaagactg
     1561 cgttgtgacg atgcaacaga tcggtggcac gatgaaggcg gaagtcgcgc agatgatgcg
     1621 tgaaggaaag cccattcagc cgatcagtct cgattcctcg gatcatcggg agttctacaa
     1681 gtttctcgaa atgcagaatg tgaatctcga agatgattct ctccctgatg ggtgggtcaa
     1741 cttctatcgc gaggatgact ggtgtgccac ggcgttcttc tatctcaaca agccggagaa
     1801 cggtcttcct gcgttggcag actatgccga gcgcgtggcg tacatctctg acaaacccga
     1861 aggcgcaccc cagtccaacg aggcgatcaa cgtgtaaagg caaatgctat tagagagatt
     1921 cttcacttcg ccttgctccg ttcagaatga cggactatgc gactgggacc tatcacagat
     1981 gcctattgcg tgaaatgatc gcgttccgca cgaacgccag gatcacaaat agaacacaac
     2041 cgattccaaa cacgtcgccg aaacggcggt agaagctgct cagctcgggc accggaacgc
     2101 ttcgacagtc cacccaaggt tgctcaaacg gagatgtttc tccctggatt tcacctgcgg
     2161 ctgagacata agctgaaacg ccggtattgg tcgcacgcac catgggaacc ctggtttcga
     2221 tgcaccgata gatccccaaa gcaaggtgct gttcggtctg acctatgtcc tgataccagc
     2281 catcgttcgc ggaattgata atcagcctcg cgccctcacg gacaaaccgc cttgccagat
     2341 tcggatagag aatttcgaaa cacacctgag ccgccattcg aacgtccttg agcgtccata
     2401 tctggaactc tcttccgggc tgcgtgttca gattggggtt ggcattctgg aagacacgct
     2461 tgaggacagg aaacacgtta gagaatggga tgtattcccc aaacggcacg agtttgtgtt
     2521 tgaaatgctc tcccaacggc gcgccatcgg atccaagcag g
//
LOCUS       DAOQUC010000498         2559 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_48918_length_2559_cov_3.773562, whole genome shotgun sequence.
ACCESSION   DAOQUC010000498 DAOQUC010000000
VERSION     DAOQUC010000498.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2559
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48918_length_2559_cov_3.773562"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..679)
                     /locus_tag="PKH07_09235"
     CDS             complement(<1..679)
                     /locus_tag="PKH07_09235"
                     /inference="COORDINATES: protein motif:HMM:NF015604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046559 - alpha-glucuronidase activity
                     [Evidence IEA];
                     GO_process: GO:0045493 - xylan catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucuronidase family glycosyl hydrolase"
                     /protein_id="HNT35166.1"
                     /translation="MYHLFCFLLLMSMTCCLGASADSSRTVNLVKNGISRYRIEIPRN
                     PSPAVSYAAEELQRFVEECTGARLPVTAEERFAFAPSVLIGPCRKSRKAGLLEQAAAL
                     PEDGVIIRSLGRDFALLGQNERGNLYSIYVFLERYLGVRFLAWDQMHVPKRTDLSLPR
                     INFSYAPPFMYRETLYYDSFPKEIAARQRLNGPTTKCDESVGGKIDFFPYVHSFNDLI
                     PEKEYFEQ"
     gene            complement(826..>2559)
                     /locus_tag="PKH07_09240"
     CDS             complement(826..>2559)
                     /locus_tag="PKH07_09240"
                     /inference="COORDINATES: protein motif:HMM:NF037878.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35167.1"
                     /translation="MIVNGHGNEAQLNCHLLSMAIALYDEDPVPYQYCAYRILEELVP
                     MRRFEYQSPRHNQGIGYGIFRFCWDMHAAWLFYRMSGERVFDDNIEDVRNYWLYMRLP
                     NEQSLPDGDGNADGRKANLGATALLTYAYANDPIMKRDFVRQGGLRYDPILVLLLNDP
                     DLKPADDFSSLPLTIDFGPILGSMVARTGWNMGEGASDVVVEMKGGGYHFGNHQHPDA
                     GSFQIYYRGRQVVDLGQYKFYGTPYDTNFNKRSISHSMMLAYDPEEKFLDGKLVNDGG
                     GIYRNYCPPTPEHAQTERRCDNGRILSADFGPSKQRPFFCYFSVNLTAVYSEKIKDYV
                     RTFCFLNLDDDTHPAALIILDNMATVNPSFKKYWQINTLNPPELTKDGVILRNSDLGL
                     DGKVTVRMLRPHPQERYVEILSGTESTTVFGNYFEAPKPDGAEANGHRIMFSPSKEQA
                     SDLFLTVMTMSDAKAADLPVSVEEFPDAFVVSLADRVVLMNKTGQMLTKALEVAVNAD
                     GESQLLLTGLAPGAWSIRSEDGKIQHNTQVEPNKNTAFFVVPRGHYVTKPEAIPGAAE
                     FQPKPDLMPQL"
BASE COUNT          620 a          677 c          651 g          611 t
ORIGIN      
        1 gctgctcgaa atactccttc tcgggaatga ggtcattgaa agagtgaaca tacggaaaga
       61 agtctatctt cccacccacg ctttcgtcgc atttcgtggt agggccgttc aaccgttgtc
      121 ttgcggcgat ctcttttggg aaggagtcat agtacaatgt ttctcgatac ataaaaggag
      181 gcgcgtagga gaagttgatt ctcggcagtg acagatccgt tcgcttgggt acatgcatct
      241 gatcccacgc tagaaagcgg acgcccagat acctctccaa aaagacgtag atgctgtaga
      301 gattcccgcg ttcattctga ccaaggagag caaaatccct cccgagagaa cggatgatga
      361 ctccgtcctc gggcaacgct gcggcttgct cgagaagtcc tgccttacgc gacttcctac
      421 acgggccaat cagaaccgac ggtgcgaagg caaaccgctc ctcggcagtg acgggcagcc
      481 ttgcaccagt gcactcctcc acaaaacgct gaagttcctc agccgcatag gaaaccgctg
      541 ggctggggtt gcgaggaatc tcgattcgat acctgctgat gccgttcttc accaagttga
      601 ccgttcgtga tgaatcggca gaggctccca ggcaacacgt catggacatg agcaacagaa
      661 agcaaaacag gtggtacatt ctctcctcct cgatgtgagc ggagtacatc ctcttctgtg
      721 tgagacagcc ttcagagcca gatcattccg gattcggcag cgttgtaacg ccgctcttac
      781 tgatgcacat cgagccactt ttcctgtata gcacaacgtg cagcattaca attgcggcat
      841 aagatcaggt ttcggttgga actcggctgc gcccggaata gcctctggtt tggtgacgta
      901 atgtccacgt ggaaccacga agaacgccgt gttcttgttg ggttccactt gtgtattgtg
      961 ctgaatcttt ccatcctcgc tacggatact ccaggctccc ggagccagac cggtcagtag
     1021 taactgagat tccccgtccg cattgacagc cacctccaat gcttttgtca gcatctgtcc
     1081 tgtcttgttc atcagtacaa ctctgtcggc aagactgaca acaaaggcat cagggaactc
     1141 ctcgacactg acaggcaaat ccgcagcctt ggcatcggac atcgtcatga cggttaggaa
     1201 aagatcgctc gcctgctcct tgctgggaga gaacatgatc cgatggccgt ttgcttccgc
     1261 tccatcgggt ttgggtgctt cgaagtaatt gccaaataca gtcgtggact ccgtgccact
     1321 cagaatctca acatatcgct cttggggatg aggacgcagc atccggacag tcaccttgcc
     1381 atctaagcca agatcgctat ttctgaggat gacaccgtcc ttcgtcagct caggtgggtt
     1441 aagtgtattg atttgccagt acttcttgaa tgagggattt accgttgcca tattgtcgag
     1501 gatgatcagc gccgccggat gcgtgtcatc atcaagattc agaaagcaga acgtccgaac
     1561 atagtccttg atcttctcgc tgtagactgc tgtcaagttg accgagaagt aacagaaaaa
     1621 cggacgttgt ttggaaggtc caaagtctgc cgagaggatt ctcccattgt cgcagcgacg
     1681 ttctgtctgt gcgtgttctg gcgtaggtgg gcaatagttg cgatagattc ccccgccgtc
     1741 gttgaccagt ttgccatcca ggaatttctc ctcgggatcg taggcaagca tcatactgtg
     1801 tgagatggag cgcttgttga aattggtatc atagggggtt ccgtagaatt tgtactgtcc
     1861 caagtcaaca acctgacgac cacggtagta gatttggaaa cttcctgcgt ccggatgctg
     1921 atggttgcca aagtgatatc ccccgccttt catttcaacc accacgtcgg atgcaccttc
     1981 acccatattc caaccagtac gtgcgaccat tgaaccgaga atcggaccga aatcaatagt
     2041 gagcgggagc gaactgaaat catctgcagg cttcagatcc ggatcgttca gaaggagaac
     2101 cagaatagga tcgtaacgca agccaccctg acggacgaag tcgcgtttca tgatcgggtc
     2161 gttcgcataa gcatacgtaa ggagtgccgt tgctccgaga ttggccttcc tgccatcagc
     2221 atttccgtcg ccatcgggaa gactctgttc gttgggcaga cgcatgtaaa gccagtaatt
     2281 gcgcacgtct tctatgttgt catcaaaaac ccgttcaccg ctcattcgat agaacaacca
     2341 cgcggcgtgc atatcccaac agaatcgaaa tatcccgtac ccaattccct gattgtgacg
     2401 cggggattgg tactcaaacc gccgcatcgg aaccaactcc tcaagaatgc ggtaggcgca
     2461 gtactggtag ggaacaggat cttcatcata caaggcaatt gccatagaga gaagatgaca
     2521 gttcagttgc gcctcattgc catgaccatt gacgatcat
//
LOCUS       DAOQUC010000499         2559 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_48927_length_2559_cov_2.945687, whole genome shotgun sequence.
ACCESSION   DAOQUC010000499 DAOQUC010000000
VERSION     DAOQUC010000499.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2559
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48927_length_2559_cov_2.945687"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2220)
                     /locus_tag="PKH07_09245"
     CDS             complement(<1..2220)
                     /locus_tag="PKH07_09245"
                     /inference="COORDINATES: protein motif:HMM:NF039643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6067 family protein"
                     /protein_id="HNT35168.1"
                     /translation="MTTLSRTLTLLAALLAARCASTPTEVDDMFPKTTIPVISILEER
                     CNDDDLAVFLPERLELVCRAQPRDQWGYCFTGFETNWNGPIELEVQGSGDYKIHNHLS
                     TAALSLDFGATGGWSERSLIGLGLLQGWRPPHAPSWGAGWNGQKIVRGNLLNAGPEKQ
                     KIVVTPSDYAPEDWDGRLWIGLLLHNTGASTTLKARITNALPKSGNLADSDPDEEYLW
                     TAMREHQIAYIKPAVKELLRYESRLRLPDTSREVKFYAQEIIDNAPRPVAIRKIEAAL
                     AQAKQGPIPAAFFMEAVSSLLKADTDDDRPEAIAQLNRLYETWHETGAFGKELGCIIR
                     TATNLDKIALRDCGTGEVLAAPAAAIRLSAARHEYEGFQIILTPLPDSVSKLKLAVSD
                     LRSGDSVIPSTNITVNPVGYTRVLQHQGDGILCPDPLLIGDIPDLSCGEHQPVWITVY
                     VPENALPGLYEGTVTLSSPERDAVAQVPIEIRVRNFNIPKRISLRTSFWMFREQLNRF
                     YHLDEIPLDDYMKWIDFALQHRLCPIDAEEGACIPMIDLVLAEKTSDGLDMLRANPSP
                     FFGKWDTYLDHLISGGASTIHLAPSQHFGTWYATQQNPVGSEEQLRQVVDALRVLREH
                     CRERGLFDMHYLQLRDEASTPDSLAVYRRVREELPDLKLLLTWPLGEAVSLIDIPCPI
                     TSGFTEPWRDETKANGGEFWWYVAWIPQGEYANLFIEQTGPQNRALFWQTWKYKVDGL
                     "
BASE COUNT          565 a          652 c          739 g          603 t
ORIGIN      
        1 gagtccatca actttatact tccacgtctg ccagaagagg gcgcgattct ggggccccgt
       61 ctgttcgata aaaagattgg cgtattctcc ttggggaatc caggcgacat accaccagaa
      121 ctcgccaccg ttcgccttgg tctcatcgcg ccaaggttcc gtaaaccccg aggtgatcgg
      181 acaggggata tcaatgagag agaccgcctc accaagaggc caggtgagaa gcagtttgag
      241 atccggcaat tcctctcgca cccgacgata gacagcaagg gagtcgggag tgctggcctc
      301 atcgcggagc tggaggtagt gcatgtcgaa gagcccacgc tctcggcaat gctcacgtag
      361 aactcgtagc gcatcgacca cctgacggag ttgttcttcc gacccgacag gattttgttg
      421 ggtggcatac catgtgccga agtgctgaga aggagcgaga tggatggtgc ttgccccgcc
      481 ggagatgaga tgatcgaggt aggtgtccca tttcccaaag aacggcgacg gatttgcgcg
      541 aagcatgtct agtccgtcgg acgttttttc agccagaacg aggtcgatca ttggaataca
      601 ggccccctcc tccgcatcga taggacagag ccggtgctgc agggcgaagt cgatccattt
      661 catgtaatcg tcgagaggta tctcatccaa gtggtagaaa cggttcagtt gctctctgaa
      721 catccagaaa ctggtgcgca aggaaatcct cttcgggatg ttgaaattcc taacgcgaat
      781 ctcgatggga acctgtgcaa ctgcatctct ttccggtgag gaaagggtga ccgttccttc
      841 gtagaggccc ggaagtgcat tctccggaac atagaccgtg atccaaacgg gttggtgctc
      901 gccgcaggaa agatcaggaa tgtctccgat cagcagagga tcgggacaaa gaataccatc
      961 gccctgatgt tggagcactc tcgtatatcc aactgggttg accgttatgt ttgttgaagg
     1021 gatcacggag tcgcccgatc gcagatcgga aacagccaac ttcaacttgg agacagagtc
     1081 cggcagagga gtaagaatga tttggaaacc ttcatactcg tgccttgctg cgctgagccg
     1141 gatggccgct gccggagctg ccaggacctc acccgttccg cagtcccgta aggcgatctt
     1201 gtccaagttt gtggcggtgc gaatgatgca ccccaactcc ttgccgaagg ccccggtttc
     1261 atgccatgtt tcgtaaaggc gattgagttg tgcgatggcc tccgggcgat cgtcgtccgt
     1321 gtctgctttg agcagggaag acacagcctc catgaaaaaa gccgccggaa tgggaccttg
     1381 ttttgcctgt gcaagggcgg cttctatttt ccggatagcg actgggcgcg gagcgttgtc
     1441 tatgatctcc tgtgcataga acttcacctc acgcgacgta tcgggaagcc tcagacgcga
     1501 ttcatatcgg aggagctctt tgactgctgg cttgatatag gcaatctgat gctcgcgcat
     1561 tgccgtccag aggtattctt cgtctggatc tgaatccgcc aggtttcccg attttgggag
     1621 cgcgtttgtg atgcgcgcct tcagggtggt tgaagccccc gtgttgtgaa gcagaagtcc
     1681 aatccagaga cggccatccc agtcttcagg tgcgtaatcg gagggggtga cgactatttt
     1741 ctgcttctcg ggtccagcat taaggagatt acctcgaacg atcttctgac cgttccaacc
     1801 tgccccccag gaaggtgcgt gtggaggacg ccagccctgg agaagtccga gaccgatcag
     1861 gcttctctcg ctccagccac ccgtcgctcc aaaatccagt gacaaggcgg ccgtggatag
     1921 atggttatgg attttgtagt cgccgcttcc ttgcacttcg agttcgatag ggccgttcca
     1981 gttggtctcg aagcctgtga aacagtaacc ccattgatcg cgcggttgcg cgcgacagac
     2041 caactcaagg cgttccggca gaaatacagc aagatcgtca tcattgcagc gctcctccag
     2101 aatgctgatg acgggtatgg ttgtctttgg gaacatgtcg tctacctccg tgggtgtcga
     2161 agcgcatctt gcggcaagca atgcagccag aagcgtgaga gtcctcgata atgttgtcat
     2221 tctgtcctcc tcggtgcagt atggtgagta tagagactcc cagtggagcc aggcaaggag
     2281 atttgtcccc agtcagtatt tgattccacc ttgccttacg aattgattac gctatactcg
     2341 ccagtcatat ccaaggtttt aaaggacctt cttcgcatat tgcagacgtt ggaggagatg
     2401 gaaagatgat cgtgaagaga cgttttggat tgcctccgct ttttctgatg gttttgactt
     2461 tcggtctggg gttagtcgca aagacaaatg gaggacaggc acagatgagc aagtttgatg
     2521 gagttttctg tgagggggag ggcgactccc agtggttcc
//
LOCUS       DAOQUC010000500         2556 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_49006_length_2556_cov_3.275890, whole genome shotgun sequence.
ACCESSION   DAOQUC010000500 DAOQUC010000000
VERSION     DAOQUC010000500.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2556
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49006_length_2556_cov_3.275890"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            303..563
                     /locus_tag="PKH07_09250"
     CDS             303..563
                     /locus_tag="PKH07_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011937518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HNT35169.1"
                     /translation="MEFEFDTRKSAANKRKHGLDFVEAQALWEDPDLVEIPARTEDEP
                     RFLVIGKTTGKHWSGVITYRDDRIRIISFRRSRKEEVEIYES"
     gene            553..783
                     /locus_tag="PKH07_09255"
     CDS             553..783
                     /locus_tag="PKH07_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015163396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family antitoxin"
                     /protein_id="HNT35170.1"
                     /translation="MKAKDFDKAFDSDEDVTQFLDFSKARRPEQEQKRVNVDFPVWMI
                     QSLDKEAKRLGVPRQSLIKVLIAQHLEHATAR"
     gene            946..1395
                     /locus_tag="PKH07_09260"
     CDS             946..1395
                     /locus_tag="PKH07_09260"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HNT35171.1"
                     /translation="MKIILIVVGVLAVVAISVWLQKLHDAGLRRLAKEFMGNRPRRSS
                     REFGQTFYPDHAEVAATVRDILAQHHPIDLAQLEPSDQPVADLKMDDLDSMSTVEFVI
                     AVEKHFGITIKDADAEKMRTFDDICTHVIAKLKEKEVDNNSVEHIHA"
     gene            1432..>1757
                     /locus_tag="PKH07_09265"
     CDS             1432..>1757
                     /locus_tag="PKH07_09265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35172.1"
                     /translation="MKKMESIQRRLEAKTRRWWFFVALVAIQFLVLPFSSKGFFPEKT
                     GEIIGYTLGHAIWFECVPAFPVFQIVAIVLFTTIPVLRNKMRVVLAVHAGISYVLFAI
                     LQNVAVA"
     assembly_gap    1758..1767
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1768..2271
                     /locus_tag="PKH07_09270"
     CDS             <1768..2271
                     /locus_tag="PKH07_09270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35173.1"
                     /translation="PRFGLSIVTVNVVMFLMVAATWFLEAAVVRNDLSSRRRHPWQFA
                     AIAPAVLAFWLPINWSTGLPDFRLLYFLTSGSALAFCMMTPVYLAVLIIVYPQVNLLT
                     LRVTSSVGVIMGLYNVIPKLVLRAYSSWWDGALHIPLLLLSVWGVALSMTKLEAAGHN
                     EQILRTS"
BASE COUNT          585 a          695 c          689 g          577 t
ORIGIN      
        1 caaatacgac gggcgattta ccgtctacct taccgatggc accaagaagg acctgtccat
       61 cagtaactgg gtcggtcttc aacacgttgc tggcaagacg gatctcggcg attacaagat
      121 catgatagcc gaattgaagc tgctgaaggt ggtccgcacc aagaagtgat gatgtcggca
      181 tgaatggccc aacaaggctc gggaggcgac gcggtgcccg cgcgcctcag tcccgccgtt
      241 cgaatcaaga agaagaaccg ttctattgac attcaccacc tggcgggtat attatctata
      301 ccatggaatt tgaattcgac acacggaaga gtgcggcaaa taagcgaaag cacggcctcg
      361 actttgtcga ggctcaggca ctgtgggaag acccggatct cgttgagatc ccggctcgaa
      421 cagaggacga gccgcgtttc cttgtgatcg gcaaaaccac cgggaagcat tggtccggag
      481 tcatcacgta ccgtgatgac cgcatacgga tcatctcgtt tcggaggtcc cgaaaggaag
      541 aggtggagat atatgaaagc taaggatttt gacaaggcgt tcgattccga cgaggacgtt
      601 acgcaatttc tcgatttctc aaaggcacgc cgccctgagc aagagcagaa acgcgtgaat
      661 gttgacttcc ctgtctggat gattcaatca ctagacaaag aagcaaagcg tctcggagtt
      721 cccagacaat cactgatcaa ggtgctgatt gctcagcacc ttgagcatgc aacagcacga
      781 tgaagaaatt cgaaccagcc cctgcacggt acgtcgctac cgcgccgccc gtgaggggtg
      841 gcgttcggtg ttttccccga agccgcttcc gaaaactggg tcttatgccg accggaaatc
      901 agataaaggt tcttatacgg aaaccatata tacattgttg tccacatgaa gataatattg
      961 atagtcgtgg gtgtcctcgc cgtggtcgcc atttcggtct ggctccagaa acttcatgat
     1021 gccggactga ggcggttggc caaggaattc atgggtaacc gaccacggcg atccagtcgt
     1081 gagttcggcc agaccttcta tccagaccat gctgaggtcg ccgccacagt gcgagacatc
     1141 cttgcacagc atcacccgat tgacttggcc caacttgaac cgtccgatca acctgtggct
     1201 gacctcaaaa tggatgatct ggactcaatg tccacggtcg agtttgtcat cgcagtcgaa
     1261 aaacactttg gcatcaccat caaagatgct gatgccgaaa agatgagaac gttcgatgac
     1321 atctgcaccc acgtaatcgc gaagctgaaa gagaaggaag tggacaacaa cagcgtggag
     1381 catattcacg cgtagccgcg cgaaatgctc acgctggacg ttgtgacgac aatgaagaag
     1441 atggaaagca tacagcgtag gcttgaagcc aaaacacgac ggtggtggtt cttcgtcgcc
     1501 ttggtggcga ttcaatttct cgtcttgcca ttctcgtcca aggggttctt ccccgaaaag
     1561 actggtgaga tcatcgggta cactctcggg cacgccatct ggttcgagtg cgtgccggct
     1621 ttcccggtgt tccagatcgt cgcgatcgtg ctgttcacca cgattcccgt tctccgcaac
     1681 aagatgcgtg tcgtgcttgc tgtccacgca ggcatctcct acgtgttgtt cgcgatcttg
     1741 cagaatgtcg ccgtagcnnn nnnnnnnccc aggttcggat tgagcatcgt gaccgtcaac
     1801 gtcgtcatgt tcttgatggt cgcagcaaca tggtttctgg aggcagccgt cgtccgcaac
     1861 gatctctctt cgcgcagacg ccacccatgg cagttcgcag caatcgcccc cgctgttctt
     1921 gcgttctggc tcccgatcaa ctggagcacg ggcttgcccg actttcgact cctgtatttc
     1981 ctgacatcag gttcggccct ggctttctgc atgatgacac cggtgtacct ggcagtgctc
     2041 atcatcgtct acccgcaagt gaacctcctc acgctccgtg ttacaagctc cgtcggggtg
     2101 atcatggggc tgtacaacgt tatccctaag ctggtgttgc gagcctacag ctcgtggtgg
     2161 gatggagcac tccacattcc actgcttctt ctctccgtgt ggggagttgc cctgtcgatg
     2221 acgaaactgg aagcggcagg acacaacgag cagatactcc ggacctcgtg acctcggcgg
     2281 gagatcttta cgttggcttg ccgaatgcga caaggataga aagccgcagg ctgttaccag
     2341 ttccaaatct tgttgaagtc tttcgttgcg gtgttttccc caaagccagt agtcggcggg
     2401 attcatcccg cgcagcataa atgctgccct acggggttac cctcaaccca ggatcccggt
     2461 ttgcggggga aaccgggcta cttgcttcga ccaagcagac gcgccttgat caggtgtcga
     2521 tcaggctgcg ttctcccagc acaatgttgt caatga
//
LOCUS       DAOQUC010000501         2555 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_49038_length_2555_cov_3.499200, whole genome shotgun sequence.
ACCESSION   DAOQUC010000501 DAOQUC010000000
VERSION     DAOQUC010000501.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2555
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49038_length_2555_cov_3.499200"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..908)
                     /locus_tag="PKH07_09275"
     CDS             complement(<1..908)
                     /locus_tag="PKH07_09275"
                     /inference="COORDINATES: protein motif:HMM:NF012861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HNT35174.1"
                     /translation="MNQQQPLLQQYRRAWEYLVKWSIISVSIAIPAALSVWALRTFVD
                     ILFMRLCGKASALLFFMPAVGGLVVGLILYRIDHRVSGEGVPRYVKAVLENNGAIPFR
                     IWPLKILATITSLGFGGSGGLVGPLAYAGGALGSSVGRALKKLPFLLPLQQSDLKVAT
                     VCGVGAALGALLKAPVAGAILAGEILYTASLSYRDLFPAMLASACSYLVYCFATDFKS
                     FFDVAPFANSPQMVGYSALVGLAGGFVGILFVQTLRVVRHGFDRCFLPIVLRPAAGGV
                     IVGILAWLVGREIMSVGRPLFHVAVTG"
     gene            complement(913..2202)
                     /locus_tag="PKH07_09280"
     CDS             complement(913..2202)
                     /locus_tag="PKH07_09280"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNT35175.1"
                     /translation="MPLTVTANEELILLVDDNRPYREAVKRNLEFIGYAVEEAEDLDS
                     ALVQLRRTRPMLIITDLDMRTHTEGLDLIRTVKEQYPRIPVILISAVGTFDEGALARE
                     LGALYVISKSRIDEEIENLYQCLDDIKGMLRRLRGVRERVEAYLFGDTQISRSSLLEE
                     IEQSIADPGVDMGLKGDLFDMMNQIQSVEREKEVKAELEKLGDLPAAPNFANLQTSLK
                     KAIPRWDQLHEESRSMMETAEIFKAIEESDSTLSVSRNVGFSYCFAVENEIKQRIGRK
                     IARFLSSKQFVTLLQQLYDTSLGNLDLHFTQYLMISKHIRQDDLNLDIIRQVIERMNK
                     HRDRYKPDGLKALGVVLFCYGRNHQFKSARQAVQIENPLGLKGLTDDEVTDLAAALIR
                     LQHFRNPYIHPEFSEHEKTDRLREVALESLILASQLS"
     gene            complement(2330..>2555)
                     /locus_tag="PKH07_09285"
     CDS             complement(2330..>2555)
                     /locus_tag="PKH07_09285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35176.1"
                     /translation="ELAADLALAPDGTGYYILDAYGGIHVSSPDLPILTTRYDDREQW
                     ALDLEILSDGSAYVLSRDGMIYHCKSSNEN"
BASE COUNT          638 a          713 c          652 g          552 t
ORIGIN      
        1 cctgtgacgg caacatgaaa caagggtctg cccacactca tgatctctcg tccaacaagc
       61 caggcgagga ttccgacgat cacgccgccc gctgcagggc gcagtacaat gggcaagaaa
      121 cagcgatcga agccgtgacg aacaacccgt agagtctgca caaacaagat cccgacaaat
      181 ccacccgcaa gtccgactaa ggcgctgtag cccaccatct gtggcgaatt ggcaaatggg
      241 gcaacatcga agaaactctt gaaatccgtg gcaaaacagt agaccaaata actgcatgca
      301 ctggcgagca ttgcaggaaa caagtcccga taggagagcg atgctgtgta gagaatctcc
      361 ccggcgagaa tcgctcctgc aacgggcgct ttgagcagtg ctcccagtgc tgcacccaca
      421 ccgcagacag tggcgacttt gagatcgctc tgctgcagag gcagaaggaa aggaagtttc
      481 ttcaatgcac gtccaaccga cgaaccaagc gcacctcccg cataggcaag aggcccgacg
      541 agtcccccgc tgcctccaaa gcctaggctt gtgattgtgg caagaatctt caatggccat
      601 atccgaaacg ggatggcgcc attgttctcc agaactgcct tcacgtagcg cggaacgcct
      661 tcaccactca cgcgatgatc aatgcggtag aggatcagtc cgacaaccaa accacccacg
      721 gcaggcatga agaaaagaag cgctgatgcc ttcccacaca gtcgcataaa aagtatgtca
      781 acaaacgttc gaagggccca aacgctcagc gcggcaggaa tcgcaatgct gacggagatg
      841 atgctccact tgacgagata ttcccacgct ctacggtatt gttgaagcag aggctgttgt
      901 tggttcatca actcaactca gctgcgaggc aagtatcagg ctttccaatg ccacttcacg
      961 aagtcggtcg gtcttctcgt gttcactgaa ttcaggatga atgtagggat tccggaaatg
     1021 ctgaaggcga atcagcgcag ccgccaaatc tgtcacctca tcatcagtaa gccccttcag
     1081 tccgagaggg ttctcgattt ggacagcctg tcgagctgac ttgaactgat ggttgcgtcc
     1141 gtagcagaac agcaccactc ccagcgcctt gagtccatcg ggtttgtatc ggtcgcgatg
     1201 cttgttcatg cgctcgatga cttgcctgat gatatccaga ttcagatcgt cctgacgaat
     1261 gtgtttggag atcataaggt attgagtgaa atgcaaatcg agattcccaa gactggtgtc
     1321 atagagttgc tgcaaaagcg tcacgaactg cttgctggaa aggaatcggg cgatctttcg
     1381 gccaatgcgc tgcttgatct cgttttccac tgcaaagcag taggaaaaac ccacgttgcg
     1441 agaaacggaa agcgtcgagt cggattcttc gatggccttg aagatctctg ccgtctccat
     1501 catgctgcgg ctttcctcgt gcagttgatc ccatctggga atagccttct tgaggctggt
     1561 ctgaaggttg gcaaagtttg gggctgccgg caaatcacct agtttctcga gctccgcctt
     1621 gacttccttc tcgcgttcga cactctgaat ctggttcatc atgtcgaaca agtcgccctt
     1681 cagacccatg tccacaccag ggtcggcgat ggattgttcg atttcctcca agagcgacga
     1741 acgggatatc tgcgtgtccc cgaaaagata ggcctccacc ctttcacgaa cgcctctgag
     1801 acggcgtagc atgcccttga tgtcatccag acactggtat agattctcga tctcttcatc
     1861 aatacggctc ttgctgatga catagagcgc gccgagttcg cgcgccaagg caccctcgtc
     1921 gaaagtcccc acagcggaga tgagaatcac gggaatccga ggatactgct ccttgacggt
     1981 tcggatgaga tccaggcctt cggtgtgcgt gcgcatgtcc aagtcggtga tgatcaacat
     2041 ggggcgcgtg cggcgcagct gtaccagtgc tgaatccaga tcctcggctt cctcaaccgc
     2101 atagccgatg aactccagat tgcgttttac cgcttcccgg tacggcctgt tgtcgtccac
     2161 cagaaggatc agttcttcat ttgctgtgac cgtcaaaggc acctgaacct catgaaaaat
     2221 ctgcgaatag tctcatccaa atcactatag cccatttgga tatcaaagac catatctata
     2281 taaaaatgcc aatccagagg aattctgtca acccggcacc gaaagatgct cagttctcgt
     2341 ttgacgactt gcagtggtag atcatgccat ccctcgacag cacgtaggcg ctgccgtcac
     2401 tgagaatctc caagtccagt gcccattgct cacggtcgtc atatcgagtg gtcaggatag
     2461 gaagatcagg cgagctgacg tgaatcccac cataggcgtc cagaatgtaa tagcccgtgc
     2521 cgtcaggagc caatgccaga tcagcggcca actcc
//
LOCUS       DAOQUC010000502         2550 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_49200_length_2550_cov_2.803607, whole genome shotgun sequence.
ACCESSION   DAOQUC010000502 DAOQUC010000000
VERSION     DAOQUC010000502.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2550
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49200_length_2550_cov_2.803607"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>498
                     /locus_tag="PKH07_09290"
     CDS             <1..>498
                     /locus_tag="PKH07_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNT35177.1"
                     /translation="TLLNRQSGEISIDEAYGLSATQQKRGRYRMGEGVTGKVIATGEP
                     AVVPRISEEPMFLDRTGARKALDRKDISFLCVPIKLGNEVIGALSVDRLFDDSTALEE
                     DIRLLSIIASMISQAVRLRRAAQEERLRLMEENSRLREELKSRFRPSNIIGASNAMQG
                     VFDLIA"
     assembly_gap    499..508
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <509..1409
                     /locus_tag="PKH07_09295"
     CDS             <509..1409
                     /locus_tag="PKH07_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018526269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNT35178.1"
                     /translation="QVSRSDATVLIRGESGVGKELVANAIHYNSNRANKPFVKVNLAS
                     LPETVVESELFGHERGAFTGALTQRKGRFELADGGSIFLDEIGDLSPSMQVRLLRVLQ
                     EREFERVGGVETIKVNVRVISATNRNLEELVTEEAFRQDLYYRLNVFPIHVPPLRERR
                     TDILLLADHFIEKCSRANNKSVRRISTQAIDMLMAYHWPGNVRELENSIERAVLLSND
                     DVIHGHHLPPTLQTAEASGTVHQGSLQVTLAGVERDLICDALKSSRGNMAQAARALGI
                     TERIMGLRVKHFGIDLKRFRTQM"
     gene            1980..2534
                     /gene="rfbC"
                     /locus_tag="PKH07_09300"
     CDS             1980..2534
                     /gene="rfbC"
                     /locus_tag="PKH07_09300"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015350608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HNT35179.1"
                     /translation="MRITPVEIPGILVIEPDVFRDSRGLFLETYHQAKYFQSGIREPF
                     VQDNRSRSLKNVIRGLHAQRQQTQGKLIHVIEGEIYDVAVDVRRGLPTFGKWFGIKLS
                     QDNFKQVFIPPGFVHGFAVLSDFAQVEYKCTDFYRPEEEITIRWNDPDIGIDWQIKNP
                     ILSDKDRNAPLLNEVMDILPIVKA"
BASE COUNT          603 a          630 c          674 g          633 t
ORIGIN      
        1 gaccttgctc aacagacagt cgggggagat ttccattgac gaggcgtacg gtctctccgc
       61 aacccagcag aagcgtggca ggtatcggat gggggagggt gtcaccggta aggtgatcgc
      121 caccggtgag cctgcagtag tgcctcggat ctccgaggag ccaatgttcc tggatcgaac
      181 aggagcacga aaggctctcg acagaaagga tatctctttt ctctgtgttc ccataaaact
      241 gggcaacgag gtcatcggtg ccctgagtgt agaccgattg tttgatgatt caactgcttt
      301 ggaagaggat attcgacttc tttcgattat cgcatccatg atctcgcaag cggttcgtct
      361 gcgtcgtgct gcccaggaag aacggctccg actgatggag gagaactcac gccttcggga
      421 agagctcaag agtcgttttc gaccttccaa tatcatcggt gcttccaacg cgatgcaggg
      481 ggtatttgac ctcattgcnn nnnnnnnntc aggtctcccg aagtgatgct acggttctaa
      541 tccgggggga aagcggtgtt ggcaaggaat tggttgccaa cgcgattcac tacaacagca
      601 accgtgcaaa caaacctttt gtgaaggtga atcttgcctc tctgcccgaa acggtagtcg
      661 aaagtgaact ctttggtcac gaacgcggtg ccttcacagg ggctctgacg caacggaagg
      721 gaagatttga acttgcggac ggtggaagca tctttcttga cgagattggg gatctatccc
      781 cttccatgca ggttcgtttg cttcgcgtcc tgcaggagcg tgagtttgaa cgcgtcggcg
      841 gagtggagac gatcaaggtg aatgtccgtg tcatctcagc caccaacagg aatcttgaag
      901 aacttgtgac cgaagaagca ttccgacagg atctctacta tcgcctcaac gtttttccga
      961 ttcatgtgcc tccgcttcga gagaggcgga ctgacattct gctcttggcg gatcacttca
     1021 ttgagaaatg cagccgtgcg aacaacaagt ccgttcgccg aatctcaact caggccatag
     1081 acatgctgat ggcatatcac tggccgggga atgtccgtga gctggagaac tccatcgaac
     1141 gagccgtatt gctctcgaat gacgatgtca tccatggaca ccatcttccg cctacccttc
     1201 aaactgctga agccagtgga acggttcatc aaggctctct tcaggtgacg cttgcaggtg
     1261 ttgagcgcga cctcatctgc gacgcgttga agtcctctcg aggcaacatg gctcaggcgg
     1321 ctcgggcact gggcatcaca gaacgcatta tgggtctcag ggtgaaacac tttgggatcg
     1381 atctcaagcg atttcgaaca caaatgtagt ttcctgttgc ctatcctaca ttttggtagc
     1441 gcattgtgtt ccatctgggc aagacgcaat gccctatgtc tccataattt attgatttca
     1501 aagatcttaa cagttcatcg ggcagatggc atgtgatctg ctaatgaatc cttcaaccag
     1561 aggggactga ggccttcaag aaaaacacct ctcctcatgc cccgaggtat atggggtgga
     1621 ccgccagaat ccaccccttt ctctttctgt cgttctggat caagcgagtc cctgagcggt
     1681 gtacaggatc tcctctctgc agatcagaga gacaggacat ctatcttcga acggctagac
     1741 agacgggtga cagaaccttg tggcaattca gttgcactct tctcgaacaa tccgtagtat
     1801 tgctttggat gaacggaaac gctctcttct tatgatagaa aaactcgggt acttgccgag
     1861 agcgcgttag catcttaacg tgctgggact gtgcccgctt aagtgcgatg actgcaggct
     1921 caagagaccg ttcccttggg agtccggttt cctgggagca acacaaagga ggcagttcga
     1981 tgaggattac gcctgtggaa atccctggta ttctggtgat cgaaccggac gttttcagag
     2041 actcaagagg actttttctg gaaacgtacc atcaggcaaa gtactttcag tcgggcattc
     2101 gagagccctt tgtacaggac aatcgttcgc gatcactcaa gaatgtcatt cgaggtctcc
     2161 atgctcaaag acaacagacg caggggaagc ttatccatgt tatcgaaggg gagatttacg
     2221 atgtcgctgt ggatgttcgc cggggcttgc ccacctttgg aaaatggttt ggcatcaaac
     2281 tctcacagga caatttcaaa caggtgttta tcccacctgg atttgttcat ggttttgccg
     2341 ttctcagtga ctttgcgcaa gttgagtaca aatgcactga tttctaccgt ccggaagagg
     2401 agattaccat tcgctggaat gatcccgaca tcggcattga ctggcagata aagaatccga
     2461 tcctttctga caaggatcgc aacgcgcctc ttcttaatga ggtcatggat attcttccga
     2521 ttgtcaaagc ctgagacgcg actctaggct
//
LOCUS       DAOQUC010000503         2549 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_49226_length_2549_cov_3.570569, whole genome shotgun sequence.
ACCESSION   DAOQUC010000503 DAOQUC010000000
VERSION     DAOQUC010000503.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2549
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49226_length_2549_cov_3.570569"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2393
                     /locus_tag="PKH07_09305"
     CDS             <1..2393
                     /locus_tag="PKH07_09305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35180.1"
                     /translation="TGSDPFLYSPAINVNADGRHYLLIRMRSKLKTARIDRYPSSPAK
                     IYFTTQAQPSFSEMRSVGFVAYGTNHWNNYLVFLGDHPYWTGTVTQLRLDPCSLGGVL
                     LEIDKILFLEDVTVPEFVLNSNWNPREGETIRDLSPSLRLFDYHDNASGIKEAKFYYQ
                     EITNGTAGQWLQDGVADVTPEDGFSHTFNSLHAGAYNFSASLTDKAGNTTWRRREDQI
                     LSHITLDASAETLITVDAFKDLGEVNKKVFGNNMHTDHFSDSVSVTGEWVAAFDQRVR
                     EMGLPFHRFHLGYSSDRFTWKDCVWINGFRPDLIPDPCSGDTTTNLGPVRFGMEEFFA
                     RCEREGTVPYLTLEYRCSLDTQEAFERAVQDAADLVEYCLAPAGVNINGGTDWAQIRA
                     DNGHPAPYQIEYIELGNEAWGYDACGNVARYVTEPAGSDPAIVAERNRNMARRYMLDY
                     FLYQEAMKAVDPSVKLVACGLISIQEGMGVGERDEIAWDSELYSVGGDDLDAVQGHPY
                     YPYSAWQSDTTKLYWETLATAAQYDSVIQNRRAMVRRHAPGSYGRLQTVFSEYNVNYS
                     FGFGDPARINLDCSRKLMACVTLADVLGVCLNNSDMILGCHHWHLYKSTYYAAINKSG
                     SSYWRNGQFFILKMFNQYFGSRMLDARVTGVETYDYTKPKGCILPPQHGVPMLSAYAS
                     RNTEGDLVYLVVVNKHMTDPVTAIVSLQNYVASGVDLQTTIREFNGPTPLSYNTASSE
                     VLNITESSGTFNGIFEYTFPAHSVTNFAFSTTGETIPTPYPTNTTGVAVPWSEAYR"
BASE COUNT          613 a          661 c          675 g          600 t
ORIGIN      
        1 caacaggaag tgatcctttt ctgtattccc cagccatcaa cgtcaatgca gatggtcggc
       61 actatctctt gatccgaatg agaagcaagc tgaagactgc tcgaatagac cggtatcctt
      121 cctcaccggc gaagatctac ttcaccacgc aagcgcaacc gtccttttcc gagatgagaa
      181 gcgttggttt cgtcgcctat ggcaccaatc attggaacaa ctaccttgtg tttctcggag
      241 atcaccccta ctggacaggc acagtcactc agttaaggct cgacccctgt tctcttggag
      301 gtgtgcttct ggagattgat aagattctct ttctggaaga cgtcacagtg cctgagtttg
      361 tgctcaacag caactggaat cctcgtgaag gcgagaccat ccgagatttg tcgccgagcc
      421 ttcggctttt cgactaccac gacaatgcca gcggcatcaa ggaagccaag ttctactatc
      481 aggagatcac gaatggcact gcaggacaat ggcttcagga cggagttgca gatgtgactc
      541 ccgaagatgg attctcccat acgttcaact ctctgcacgc aggagcctac aatttctcgg
      601 caagtctgac ggacaaggca ggtaacacca cctggagacg gcgggaagat cagattctct
      661 cgcacatcac actggatgcg agtgctgaga ctctcatcac tgttgatgcg ttcaaagatc
      721 ttggcgaagt caacaagaag gtttttggca acaatatgca cacggatcat ttctccgaca
      781 gcgtgagcgt gaccggtgag tgggtcgccg ctttcgacca gcgggtcaga gagatgggct
      841 tacccttcca tcggttccat cttggataca gttccgaccg attcacctgg aaagactgtg
      901 tttggatcaa cggttttcgg ccggacttga tccccgatcc gtgtagtggc gacacaacaa
      961 caaatctcgg ccccgtgcgt tttggcatgg aggagttttt tgcgagatgt gaacgtgaag
     1021 gcaccgttcc ttacctcaca ctggagtatc ggtgttcttt ggacacacag gaggcttttg
     1081 aacgggctgt tcaggatgcc gctgatctgg tggagtattg tcttgcaccg gcaggtgtga
     1141 atatcaatgg aggcaccgac tgggcacaga ttcgtgcgga caatggtcat ccagcaccct
     1201 atcaaatcga gtacattgaa ctgggcaatg aggcctgggg ctatgacgct tgcggcaatg
     1261 tagctcgcta tgtgacagaa cctgcaggga gtgatccagc gatcgtggcg gaacgcaacc
     1321 gaaatatggc acgccgttac atgctggact atttcctcta tcaggaggca atgaaagcgg
     1381 ttgatcccag tgtcaaactg gttgcctgtg gactgatctc cattcaggaa ggcatgggag
     1441 tgggtgagcg tgatgagatc gcctgggact ccgaactata cagcgttgga ggagatgacc
     1501 tcgatgccgt gcaggggcat ccgtactacc cctacagtgc ctggcagtcc gacaccacga
     1561 aactctactg ggagaccctc gcaaccgcag cgcagtatga cagtgtgata cagaatcggc
     1621 gtgcgatggt tcgacgacat gcgcctggca gctatggaag gctccagacg gtgttttccg
     1681 aatacaatgt caactacagt tttggctttg gagatccggc aaggattaac ctggactgct
     1741 ctcgaaagct gatggcatgc gtcactttgg ctgatgtgct cggtgtttgc ctcaataatt
     1801 ccgacatgat cctgggatgt catcattggc atctgtacaa gagcacttac tatgctgcga
     1861 tcaacaagtc cggatccagc tactggcgca acggacagtt cttcatcctt aagatgttca
     1921 atcagtactt tggctccagg atgcttgacg ctcgtgtcac cggtgtggag acctatgact
     1981 acacaaaacc gaaagggtgt attctcccac cgcaacatgg agtgccgatg ctttcggctt
     2041 acgccagcag aaacaccgaa ggtgatttgg tctacctggt cgtcgtcaac aaacacatga
     2101 ccgacccagt cacagcgatc gtatcgttgc agaactatgt tgcctcaggt gttgatcttc
     2161 agacgacgat cagggagttc aacgggccga ctccgctctc ctacaacacg gcgagttcgg
     2221 aggttcttaa catcacagag tcctcaggga cattcaacgg cattttcgag tacacttttc
     2281 cagcgcactc agttaccaac tttgcctttt ctactacagg tgaaaccatt cctactccct
     2341 atcccacaaa cacgactgga gtcgccgtac catggtctga ggcatataga tgagagcgca
     2401 atgtttctga ccgtgcgtgt gcagggacac tgaccagcaa ccaggttgac ctagatagag
     2461 gatgagaggc cggagcccct acgtctcata cagaacagaa ggcaactgat gggtctgggg
     2521 tgatcatcct tgcgcgatga ctggctcaa
//
LOCUS       DAOQUC010000504         2549 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_49236_length_2549_cov_3.071371, whole genome shotgun sequence.
ACCESSION   DAOQUC010000504 DAOQUC010000000
VERSION     DAOQUC010000504.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2549
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49236_length_2549_cov_3.071371"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1564
                     /locus_tag="PKH07_09310"
     CDS             <1..1564
                     /locus_tag="PKH07_09310"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNT35181.1"
                     /translation="VEATSQKGRATFQGIAGLSSNEPHITGTDKTLIWGRWFAEDERD
                     TCLLPKTAAESLGITQQDMERGTASVDLLGLKLRVVGVFREDRDNGAPGMNDITDLNG
                     EDDTLMPTTYVFTSQVRVSDFSQGGIEAAAAFEFAYLDARSVAIVPFETVLSAGGTVR
                     SVAAAPKPNAQVDLDSFLTDLMLARSVVAYAGLLIPGSKDSDSLQSELMTKGRLKAML
                     YSSVGAASMKGLRNLAIPIAIAALTILNTLLGSVYEREKEIWTYSSVGLSPIHIACLF
                     IAEACVFGVIGSVGGYLIAQVIGKLVTTNVLSIGALALTNPFFLPGLTLNFSSTSAVA
                     SVILVMLITLLSAIYPAKRAHELATPEIERRWQLAPPTSEQWRVRMPFIVRHAHVPAM
                     NAYLFDFLDMHRQEMVGNFFVAQLSPVAPASDESLAIEFKCWLAPYDFGVSQQVRLAT
                     FPTHDEDLETFRLTLDRQSGDVQSWLRTNRAFLNELRKALLIWRTMTAEGRAHYELTA
                     QQLFKGYCDAET"
     gene            1610..>2549
                     /locus_tag="PKH07_09315"
     CDS             1610..>2549
                     /locus_tag="PKH07_09315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide transporter"
                     /protein_id="HNT35182.1"
                     /translation="MSLMDEVVREMGEDRLKGVDEPYQEGFNIKSVIGGLFLGFVMLP
                     GAMYLSLVAGAQGQMQTAGQSVTVILFTEIARRTFTRMKRQELVILHLLAGGLVVAGG
                     PFFALIWNQYLVQSSEAQAFGIVDELSKLHWLAPPIGSEALKLRSFLHKAWAPAILVI
                     IISQILDRLNHFSFGYVLFRLTSDVEKLPFPMAPIAAEGATALAESYEKEETWRWRLF
                     SIGASLGMIFGIIYIVFPTITGGITNRPIQMLPIPWIDLTVGVEKYLPAALFGIGTDA
                     AAIFVGFVLPFWLVLGQFAASIAVHMVANPILFKMGV"
BASE COUNT          547 a          686 c          699 g          617 t
ORIGIN      
        1 ggttgaggcg acaagccaga aaggtcgtgc gacttttcaa ggcattgccg gcttgtcaag
       61 caatgaaccc catattacgg gaacagataa gacactgatc tgggggcgct ggtttgcaga
      121 agacgaacga gacacctgtc tgcttcccaa gacggccgcc gaaagtctcg gtatcaccca
      181 acaggatatg gaacgaggca ccgcatcggt agacctgctt ggtctgaaac ttcgcgtggt
      241 cggggttttc agagaggatc gcgacaacgg cgcaccaggg atgaatgaca tcaccgatct
      301 gaacggtgaa gatgacaccc tgatgccgac gacatacgtt ttcacatctc aggtcagagt
      361 cagcgacttc tctcagggag gaatcgaagc cgcggcagcc tttgaattcg cctatctgga
      421 tgcgcgctcg gtggcaattg tcccgttcga aacggtgctg tcggcaggtg gcactgtacg
      481 ttcagtcgcc gcagcgccca aaccaaatgc tcaagtggac ttggattctt ttctcaccga
      541 tctcatgctt gcccgatcgg tcgttgcata cgccggcctg cttatccccg ggtcaaagga
      601 ttcggattcc ttgcagtcgg agctgatgac aaaaggacgg ctcaaagcaa tgctctacag
      661 ttcagtcggc gctgcatcca tgaagggact tcggaacctt gccatcccga tagccatagc
      721 ggcgttgacc atcctgaaca ccctgctcgg ctctgtttac gagcgcgaga aggagatttg
      781 gacttacagt tccgtcggcc tgtcaccgat ccatatcgcc tgtctgttca ttgctgaggc
      841 gtgcgtcttt ggtgtgattg gatcggtcgg tggctatctc atcgcgcaag taatcggaaa
      901 acttgtcaca acaaatgtgc tgtccatcgg tgcactggcg ttgacaaatc ccttctttct
      961 gccgggcttg acgctgaact tctcatccac cagcgcagtg gcttctgtga ttctggtcat
     1021 gttgatcaca ctcttgtcgg cgatatatcc ggccaaacgg gcacatgaac ttgcgacacc
     1081 agagatcgag cgacgctggc agctggcacc accgaccagt gaacaatggc gtgttcggat
     1141 gccctttatc gtccgacatg cccatgtccc tgccatgaat gcttatctgt ttgattttct
     1201 ggatatgcat cgccaggaaa tggtcggcaa cttctttgtt gctcaactga gccccgttgc
     1261 tcctgcgtca gatgaatcac tggccattga gttcaagtgc tggttggctc cctatgattt
     1321 cggagtcagt caacaagtcc ggctggcaac attcccgaca cacgatgagg acttggagac
     1381 ttttcgtttg accctggatc gtcagagtgg cgatgtgcag tcatggctca gaaccaaccg
     1441 tgcttttctc aacgaactgc gaaaggcgtt gctgatttgg cgcacgatga ctgctgaggg
     1501 aagagcgcac tatgagttga ccgcacaaca gctgttcaaa ggatactgcg acgcggaaac
     1561 ataacggaat gaagatgaga catgacgcca tgaccaaggg agagtgccaa tgagcctgat
     1621 ggacgaagtc gtccgagaga tgggtgagga tcgcctgaag ggcgttgatg aaccctatca
     1681 ggaaggcttc aatatcaaat cagtcatcgg aggattgttc ctcggcttcg tcatgcttcc
     1741 tggtgccatg tatctctctc tggtcgccgg tgcccaggga cagatgcaga ccgccggtca
     1801 atcggtgacc gtcattctgt tcacggagat cgcccgccga acctttaccc gaatgaaacg
     1861 ccaggaactg gtgatcctac acttgctggc gggcggtctg gttgtcgctg ggggtccctt
     1921 cttcgccctg atctggaacc agtatctggt acagtcttca gaggctcaag ccttcggcat
     1981 cgtggatgaa ttgtcgaaac tgcattggct ggcaccaccg attggctcgg aagccctcaa
     2041 actcaggagt tttcttcaca aggcatgggc acctgcgatc ctggtcatca tcatttcgca
     2101 aatcctcgat cgcctgaacc atttctcatt cggctacgtg ctgttccgac tcacttccga
     2161 tgttgaaaag cttcccttcc cgatggctcc gattgcggcg gaaggtgcaa cggcgttggc
     2221 tgaatcgtac gagaaggaag aaacatggcg atggcggctg ttctccatcg gtgcgtcgct
     2281 tgggatgatc ttcggcatca tctacatcgt atttccgacc atcacgggcg gcattaccaa
     2341 tcgccccata cagatgcttc ccatcccatg gatcgatctg actgtcgggg tggagaagta
     2401 tctccccgcg gcactgtttg gaatcggaac ggacgccgca gcgatttttg tcggctttgt
     2461 tttgccgttt tggctggtgt tgggacagtt tgcagcatcc atcgctgttc acatggtcgc
     2521 caaccccatc ctgttcaaga tgggcgttt
//
LOCUS       DAOQUC010000505         2547 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_49296_length_2547_cov_3.083066, whole genome shotgun sequence.
ACCESSION   DAOQUC010000505 DAOQUC010000000
VERSION     DAOQUC010000505.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2547
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49296_length_2547_cov_3.083066"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..865)
                     /locus_tag="PKH07_09320"
     CDS             complement(<1..865)
                     /locus_tag="PKH07_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNT35183.1"
                     /translation="MDKKGISRRKFLRRSGVAAAAFCIVPRHVLGGPGQTPPSEVITR
                     GVIGTGGMGMNHVVPNQEGQPPETLAVCDVDKDHLANGLRKAGHSCDGYSDFRRVLER
                     KDIDTIHIATPPHWHALISIAAAQAGKDIVCEKPVSKYIAEGRAVINAVQRYGRVFQI
                     GTYRRFGTYEQFGESKRLRKLISSGLLGSPLIVRVTRGQGFNWKVDQWSGKTDLVPQP
                     VPDVLDYDFWLGPAPFKPYHPHRVHGSFRGYWDYDGGGLSDMGQHYLDPIQYFLNKDH
                     TNPVEIEAIAPW"
     gene            complement(958..1539)
                     /locus_tag="PKH07_09325"
     CDS             complement(958..1539)
                     /locus_tag="PKH07_09325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35184.1"
                     /translation="MRFFPTYPCPKCGTDLGFPNIYNRSCISYRFWCQYVFRCSGCGS
                     ILRVRNLWRAAAIPCLSLVSLIVVICFSITSFPTVRPLLERNMTTSVAIFLLTIYLHL
                     IWCSARCPCLQEVSVKSNALQGRASLVTLGFALMTCLLFAFALSRESDSRSLAGLIFG
                     FLLALITLVLWISKAKVSPEEPSRRDVFRDKER"
     assembly_gap    1640..1649
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1950..>2547)
                     /locus_tag="PKH07_09330"
     CDS             complement(1950..>2547)
                     /locus_tag="PKH07_09330"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNT35185.1"
                     /translation="HPEKIGLGGATKRVTIIFTDLCGFTSVSEKMTSDQVVTMLNEYF
                     QEMLKAVSSYGGMVDKYVGDSIMVVFGLPEAGPHDALDAVHCALDMLERLEARKKSQT
                     PGFFSNLNMRTGINTGNVTAGNIGTERKQQYTVIGDSVNVACRLESLAPQNGILISDT
                     TYHEVQQEIAVRSLGSVPVKGKEEGVEVYEVLGRKAEM"
BASE COUNT          633 a          652 c          647 g          605 t
ORIGIN      
        1 gccatggagc gatggcttcg atctcaacag gatttgtgtg atccttgttc agaaagtact
       61 gaatcggatc cagatagtgc tgtcccatat cactcagtcc accgccgtca tagtcccagt
      121 agcctcgaaa acttccatgt acgcgatggg gatggtatgg tttgaacggg gcaggtccca
      181 gccagaaatc gtagtcaagc acgtccggta caggttgtgg gacgaggtct gtctttccac
      241 tccactgatc cactttccag ttgaatccct gtcctcgggt cactcggacg attagcggag
      301 agcccagcaa cccactgctg atcagtttcc taagtctctt tgactctcca aactgttcgt
      361 aggtgccaaa tcgccgatag gtgccgatct gaaaaacgcg accgtagcgt tgaacggcgt
      421 tgatgaccgc acgaccttcg gcaatgtact tgctcacggg tttctcgcag acgatatcct
      481 tgcctgcctg tgcggcggcg atggagatca gagcgtgcca atgaggcggt gtggcaatgt
      541 gtatcgtgtc tatgtctttc cgctccaaga cacggcgaaa gtcgctgtat ccgtcacagg
      601 aatgtcccgc cttcctaagt ccgttggcca gatgatcctt gtccacatcg cagacggcaa
      661 gagtttccgg tggctgtcct tcctggttgg gaacgacgtg gttcattccc atcccaccgg
      721 tgccaatcac tccacgagtg atcacctcgc ttgggggcgt ttgaccggga ccgcccagca
      781 catggcgtgg cacaatgcag aatgcagctg cagccactcc ggaacggcgc agaaactttc
      841 gacgtgaaat tccctttttg tccatagtgt gaaaaacctc cggaagcctg agaacattgc
      901 ctgcaaatat aggtatgtca gtccaaagtg gcaagggaaa atgaaaatgt tttctgtcta
      961 ccgctctttg tctcgaaaga catctcttct tgaaggttct tcaggggaaa ccttcgcctt
     1021 cgaaatccaa agaaccagcg tgatgagggc aagcaggaat ccgaaaatca gtcccgccag
     1081 actccgtgaa tctgattctc gtgacaacgc aaaagcaaaa agcaggcagg tcatcaacgc
     1141 aaaaccaaga gtgacaagcg atgccctgcc ttgtaatgca ttactcttta cagagacttc
     1201 ctgaagacaa ggacatcgag cgctgcacca gatcaagtga agatagatcg tcagaagaaa
     1261 gatggctaca ctcgttgtca tgttgcgctc gagaagagga cgtacggttg gaaaggacgt
     1321 gatagagaaa caaatcacca caataagaga aacaagcgat aggcatggga ttgcagcggc
     1381 ccgccagaga ttgcgaacgc gcagaatgct accgcatccc gagcagcgaa aaacatattg
     1441 acaccaaaac cgataactga tgcagctgcg gttgtagata ttgggaaagc ccagatcggt
     1501 gccgcatttg gggcatggat aagtgggaaa gaacctcatg gtgtcacctc atatacggca
     1561 ttctaatctc agttctcgca aagtcaatat ctgtagcaca ttgcgtcttt gagatgttgg
     1621 atctgtcttg gcggaacggn nnnnnnnnng atggaatctt gatgtgtgag ggatttgcga
     1681 aagtcgcaac cgagagcatg tcttgacaca gatttggtga gagacttctt gcaaccggtc
     1741 tgaatttgac gaggtcgtga aagacatccg gaagtacatt cgagagaaac gggaggtcga
     1801 acaagaggct tgagcgatat ctgcagatcg tgcagcgcag cgctacgctt ctgccaaacg
     1861 gctactgtga ccgttcatag agagcaccct accaaaaacg tttgcactca catgctaccg
     1921 gccaatccct gtagaagtcc tgttcatgtt cacatttccg ctttgcgtcc caagacttcg
     1981 tagacttcta cgccttcttc cttacccttg accggaaccg atcccaatga acgaacagcg
     2041 atctcctgtt gtacctcatg ataggtagtg tctgagataa gaataccgtt ttgtggagcc
     2101 aacgactcca gtcggcatgc cacattgacg ctgtcaccaa tgaccgtgta ttgctgtttg
     2161 cgttcagttc cgatattacc tgccgtaaca ttgcccgtgt ttatccccgt cctcatgttg
     2221 aggtttgaga aaaagccagg tgtctggctc ttcttgcgtg cttcgagtct ttcgagcata
     2281 tcgagagcgc aatgaacggc gtctaaagcg tcgtgggggc cggcttccgg aagcccaaat
     2341 acgaccatga tggagtcgcc tacgtactta tccaccattc ccccatacga tgagacggct
     2401 ttcagcatct cctggaaata ctcgttgagc atcgtgacaa cctggtctga ggtcatcttt
     2461 tccgagacgc tcgtgaatcc gcaaaggtct gtaaagatga ttgtcaccct ttttgttgcg
     2521 ccaccaagac ctatcttttc aggatgg
//
LOCUS       DAOQUC010000506         2540 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_49513_length_2540_cov_3.831388, whole genome shotgun sequence.
ACCESSION   DAOQUC010000506 DAOQUC010000000
VERSION     DAOQUC010000506.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2540
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49513_length_2540_cov_3.831388"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            487..>2540
                     /locus_tag="PKH07_09335"
     CDS             487..>2540
                     /locus_tag="PKH07_09335"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35186.1"
                     /translation="MGKKKTIHVLSNTHWDREWRFPFEETRFLLVDLVDWLLDLMEST
                     PEYQFYNFDSQTIFLEDYLEYRPENRERLEALIRAGRLIVGPWYTLPEMFSVGGEAVV
                     RNLLIGGRIADEFGACSKVGYTPTSYGQVSQIAQIYNSFGIDGIIFYRGIHHSECTNE
                     YILEAPDGTRILGICLSRHFCRGAFYIHVVKRTMHQPEWYQKGGFRYGEEDTLAFHLC
                     RADEDHEEEPVLLRSPFGKMCLLKHVAAGVHGAMEDVLEKATTDCLVLMDGMDSTYPN
                     PLLPKILKKANEVNPDWKFIHSSLPKFLEDLKSRIDPKKLTVLKGERRHPSPDTMFSA
                     FLKDSLSARLYIKQRNAQVERTLSRWAEPFSCVASSLTGKEYPTRPLLKAWKTLLACH
                     AHDSIGGLSPDQIHKDMMGRFDQADLIGKTITKRALGEVITQVDTSDCQSEDVLVTVF
                     NPLSFATNAVPYAYVDLPRERNIRAFSMYDHKGKKVPHQVISREDSYLIATEPSETPM
                     TFNTTKWKVAFEAKDIPAVGFKTFTVRPEPGKRTNYGSLITGTNTMENEFLKVAIQPD
                     GTLTVTDKTSGEVYSKLLHFEDAGEWGDPWTRMVPLGDRIYSSLGQPVKVQLLEDGPV
                     CAAYSIEMKWALPVGIDNASKQRTTEEKEICIISKISVKKGVPRVFIDMEVDNTVGNH
                     RLR"
BASE COUNT          619 a          682 c          659 g          580 t
ORIGIN      
        1 cgactcgcat cacattcatc ccggccttct tcatgagagc aatatcctcg tctatctgct
       61 ccagaggcca gtcttcgggg taatatgctg cacccaggta tggtggtttg acagaagcca
      121 tgtttccttg tcctcttttc tattcgagat gccgttgtgt ccgactaacg gacgaggcaa
      181 cgtgtattct aggtttttct attcggctgt caaggcacgt gcggatatct ctgtgaacag
      241 accttctgtg atctgctgtc agtcgtgcgg gatatccatg ttgaagttcc cgctgccttg
      301 acacccgatg aaggtgggtt tacctttcta tcatgaaagg aaatgaccgc ccctgtgggt
      361 ggggctaccc atatagtatg agcaccacac tcccccctgt ggcggtgtca tacagcatca
      421 gtttgtaagc ggttgcaaag gcattttggt gccgtgactc tgcggtgacc gacgaaagga
      481 aaaacgatgg gaaaaaagaa gacgattcat gtgctctcca atactcattg ggatcgcgaa
      541 tggcgattcc cctttgaaga aacgcgattc ctgctggttg acctggtgga ttggctgctc
      601 gatcttatgg agtccacccc ggagtatcag ttctacaact ttgactccca aaccatcttt
      661 cttgaagact accttgagta tcgtccagaa aaccgagagc gcctggaagc cctgatccgt
      721 gccggtcgtc tgatcgtagg gccttggtat acgcttcccg aaatgttctc cgttggtggg
      781 gaagccgtcg tgagaaatct tctcatcggc ggacgcattg cggacgagtt cggtgcgtgc
      841 tccaaggttg gttacacgcc cacgtcctac ggacaggttt ctcagatcgc ccagatttac
      901 aacagcttcg gcattgacgg cattatcttc taccgaggca ttcatcattc cgaatgcacc
      961 aatgaataca ttctggaagc acccgatggc acgaggattc tgggcatttg tctcagccgt
     1021 catttctgtc gcggcgcctt ctacattcac gtcgtcaaga gaaccatgca ccagccggag
     1081 tggtatcaga agggaggctt ccgctacgga gaggaagaca cccttgcgtt ccatctttgc
     1141 cgagcggatg aagatcatga agaagagccg gtactgctgc gttccccgtt tggcaagatg
     1201 tgtctcttga aacatgttgc tgcaggcgtc cacggtgcga tggaagacgt gctggagaag
     1261 gccacaaccg actgcctggt gctcatggac ggcatggaca gcacctatcc caatcccttg
     1321 cttccaaaaa tccttaagaa agccaatgag gtcaaccccg actggaaatt catccacagt
     1381 tcgctgccga agttcctgga agatctgaaa tccagaattg atcccaagaa actgaccgtt
     1441 ctcaaaggag aacgacgaca tcccagtccc gacaccatgt tcagcgcatt cctgaaagac
     1501 agcctctcgg cgcgtcttta catcaagcag cgaaacgcgc aggtggaacg aacgctgagc
     1561 cgttgggcag agccgttctc ctgcgttgcc tcttcattga caggcaagga atatccgaca
     1621 cgcccactgc tcaaagcctg gaagaccctt ctggcttgcc atgcccacga tagcattggc
     1681 ggactgagtc cggatcagat tcacaaggat atgatggggc gattcgacca agcggacttg
     1741 atcggaaaga ctatcaccaa gagagcgttg ggagaggtca tcacacaggt ggatacctcg
     1801 gattgccagt ctgaagacgt gctcgtgacc gtgttcaatc cgctctcatt tgccaccaat
     1861 gcggtgccgt atgcttatgt tgatctgccg cgggagagga atatccgagc cttctcgatg
     1921 tacgatcaca agggcaagaa ggtgccacat caggtcatct cacgtgaaga cagctatctc
     1981 atcgccaccg aaccaagtga aacgcctatg accttcaaca ccacgaaatg gaaagtggcg
     2041 tttgaagcaa aggatattcc tgctgttggt ttcaagacct tcaccgtgcg acccgagcct
     2101 ggcaaacgca ccaactacgg atccctgatc accggcacca ataccatgga aaacgagttc
     2161 ctcaaggttg cgatacagcc cgacggcacg ctcaccgtca cggacaagac atcaggagaa
     2221 gtctactcga aacttcttca ctttgaggac gctggagaat ggggcgatcc ctggactcgg
     2281 atggttccac tgggagacag aatctactca tcgcttggac agccggtcaa agtgcaactt
     2341 ctggaagacg gtcccgtctg cgcggcatac tccatcgaga tgaaatgggc tctgccggtc
     2401 ggcatagaca acgcaagcaa gcagcgcaca accgaggaga aagagatctg catcatctcc
     2461 aagatctcag tcaagaaggg cgttccaaga gtcttcattg atatggaagt ggataacact
     2521 gttggcaatc acaggcttcg
//
LOCUS       DAOQUC010000507         2540 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_49517_length_2540_cov_3.263581, whole genome shotgun sequence.
ACCESSION   DAOQUC010000507 DAOQUC010000000
VERSION     DAOQUC010000507.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2540
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49517_length_2540_cov_3.263581"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..370
                     /locus_tag="PKH07_09340"
     CDS             <1..370
                     /locus_tag="PKH07_09340"
                     /inference="COORDINATES: protein motif:HMM:NF012936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HNT35187.1"
                     /translation="GKYILNGLITKFLLRRVLQSQMPPRVLHQMARSRDVLFRRWIDV
                     DLRELILDTLSAARALSTTIFSRREVAKLLSRHESGESKVWPLLWSLLVLELWYLNQR
                     TQTVRVTSYPGEQHRTDGIR"
     gene            complement(397..1107)
                     /gene="tatC"
                     /locus_tag="PKH07_09345"
     CDS             complement(397..1107)
                     /gene="tatC"
                     /locus_tag="PKH07_09345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HNT35188.1"
                     /translation="MTFLEHLEDLRKCLLVSIGMVFGTTVLAFFFSDWLLKIFSKPIQ
                     GIVKLHTSSFAETFGVSIKLSVICGLFISFPVVLHQLWRFVSPGLYNKERKYLVRFII
                     WAWISFIIGGFFAYFAMIPVALQMMVRFTPAEIENTWFVSKYISLVMIMMLSCGLLFD
                     TPLVILLLSRLGIINPRKLSKYRGYALLASFIIGAVLTPPDPITQTMLAVPLYVLFEI
                     SIWLAVLLGYGTKEETST"
     gene            complement(1159..>2540)
                     /locus_tag="PKH07_09350"
     CDS             complement(1159..>2540)
                     /locus_tag="PKH07_09350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35189.1"
                     /translation="GMTLHWVSQPETFAPRVEAVPATLTDGEGALVAYIWERKDIPGI
                     RDEGNSPPLSQLVHSVKVSTCPDHLVAMRRAESLFLGRDESDDVFQILTAKLASPEMS
                     DREKTDAVYQWIQDNIQSGGSANRTVLDVLHLGEGSSLERALLARTLLDHLNIDSRIQ
                     FSFELGSESQTPPIPLGDRYGSPLLFVEDATGESRLLDLSSQYLSPGEITASFLNGLL
                     LNVDSLGYSVEPAATQQVNAGWIARDLKFKFLNLDSTEVGGELVYEGLYRPLMRQMLE
                     DRRVAERVLDQQVAKQLHGLVTEHSMPVLESNQAPRLQFKGRMPHMLIPSGSSEDNSG
                     AFEFVPITDPADLSSLVPDATREHPMVFTQWVVIQPYQATFAFEGERGGSSLDGFVFR
                     HIPDDQVLMTEFGIHVITYRLRGDVLRVRRSLIVPQQRISVDKYPRFARFCRLVDETE
                     KRKIVVERF"
BASE COUNT          637 a          688 c          651 g          564 t
ORIGIN      
        1 cggcaagtac attctcaatg ggctgatcac caagtttctg ctgcgcagag ttcttcagtc
       61 gcagatgcca ccacgcgttc ttcaccagat ggctcgcagc agggatgttc ttttccgccg
      121 gtggatcgat gtggatttgc gggaactcat tctcgacacg ctttctgcag cacgagcgct
      181 gagtacgacg atattttcac gccgagaagt cgccaaactg ctctccaggc atgaatctgg
      241 agagtccaaa gtctggcctc ttttgtggtc gcttctggtt ctggaactct ggtatctgaa
      301 ccaacgcact cagacggtga gagtgacatc ctatccaggg gaacaacata gaacagacgg
      361 aatcagatga gctggcgcat ccacctgttg ggatcctcaa gtcgaggtct cttcctttgt
      421 tccatatccc aacaagaccg caagccagat ggaaatctcg aacagcacat acaagggcac
      481 tgcaagcatt gtctgtgtta tcgggtcagg aggcgtcaag accgctccga tgatgaagct
      541 tgccagaaga gcatagcctc gatacttgga caacttcctc ggattgatga ttccgagcct
      601 tgaaagcagc aggatgacca gtggtgtatc aaaaagcagt ccacatgaca gcatcatgat
      661 catgaccagg ctgatgtact tgctgacaaa ccaggtgttc tcaatttcgg caggtgtgaa
      721 gcgaaccatc atctgaagcg ccactgggat catcgcaaaa taggcgaaga agccaccaat
      781 gataaagctg atccacgccc agatgataaa gcgaaccagg tacttgcgtt ccttgttgta
      841 gaggccaggc gacacaaacc gccacagttg atggagcaca accggaaaac tgataaagag
      901 tccgcagata acgctcagtt tgatgctgac cccaaacgtt tcggcaaagg aagaagtgtg
      961 cagtttgaca ataccctgga tgggtttgct gaagatcttc agaagccagt cagagaagaa
     1021 aaaggccagc acagtcgtcc caaaaaccat cccgatcgaa acaagaagac actttcggag
     1081 gtcttccaga tgctccaaga acgtcatgta tttctcacct ggacgtgggt ccgtcgtttt
     1141 gttctcgtcc gtgtccattc agaacctttc tacaacgatt ttgcgcttct ctgtctcatc
     1201 aaccaagcga cagaatcgtg caaaccgagg gtacttgtcc acagaaattc tctgctgggg
     1261 aacgatcagg gaacgccgca cacgcaagac atcccccctc agccgatagg tgatcacatg
     1321 aataccaaat tctgtcatga gcacttgatc atcgggaatg tgccgaaaca cgaatccatc
     1381 aagggaagat ccgccgcgtt ccccttcgaa agcaaaggtc gcttggtaag gttgaatgac
     1441 cacccactgc gtgaacacca tcgggtgctc tcgagtggca tcgggaacca gggaagaaag
     1501 atccgccgga tccgtgatgg ggacaaactc aaatgccccc gagttatcct ctgaagagcc
     1561 gctggggatg agcatgtgcg gcatcctgcc tttgaactgg agccttggtg cctggttgct
     1621 ttcgagaact ggcatcgagt gttccgtcac cagaccatgt agctgttttg ctacctgttg
     1681 atccaggacg cgttctgcga ctcttcggtc ttccagcatc tggcgcatga gtggtctgta
     1741 gagcccttcg tagaccagct ccccacccac ttctgtgctg tccagattga ggaacttgaa
     1801 cttgagatcg cgcgcgatcc aaccggcatt tacctgttgt gtcgccgccg gttcaaccga
     1861 gtatcccagc gaatccacat tcaagagcaa tccgttcaga aacgacgcgg taatctctcc
     1921 tggcgagagg tattgagaac tcagatccag aagtctcgat tctccggttg cgtcttcaac
     1981 aaacagaagc ggactcccat agcggtctcc caaggggatg gggggtgttt gagactcact
     2041 ccccagctcg aaggaaaact ggattctgga atctatgttg agatgatcca gaagcgtccg
     2101 tgcgagcagt gcgcgttcca gcgacgagcc ctctcccaaa tggagcacat ccaagaccgt
     2161 ccggttggca gaaccacctg actgaatgtt gtcctgaatc cactggtaga cggcatcggt
     2221 cttctcacgg tcactcatct caggcgatgc cagtttcgca gtcaatatct ggaacacatc
     2281 atccgactca tcgcgtccga gaaaaaggga ttcggctcga cgcatggcga caaggtgatc
     2341 tggacaggtg gagactttca cagaatgaac caactgcgac agcggagggc tattgccttc
     2401 atcacggatg cctgggatat ctttgcgttc ccagatgtac gccaccagcg cgccctctcc
     2461 atcggtcagt gtggccggaa cagcctcaac tctcggagca aaagtctcgg gttgacttac
     2521 ccagtgaagc gtcatccctt
//
LOCUS       DAOQUC010000508         2536 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_49655_length_2536_cov_3.156389, whole genome shotgun sequence.
ACCESSION   DAOQUC010000508 DAOQUC010000000
VERSION     DAOQUC010000508.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2536
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49655_length_2536_cov_3.156389"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1605)
                     /locus_tag="PKH07_09355"
     CDS             complement(<1..1605)
                     /locus_tag="PKH07_09355"
                     /EC_number="1.5.1.20"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF006396.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional homocysteine
                     S-methyltransferase/methylenetetrahydrofolate reductase"
                     /protein_id="HNT35190.1"
                     /translation="MQRVPFMDRLRSGVIVCDGAMGTLLYSKGIYINRCFEGLNLQSP
                     NLISEVHEAYLKAGAEIIETNTFGANRIKLVHHGLEDQLELINRKGVELARSIAGDTA
                     YVAGSVGPVGKSLEPFGPIALSVAKETFREHVFALVAAEVDLLILETFSNLEEIRLAL
                     ETAREITDIPIVAQMSVSESGTTVFGVTPEEIAKTLTEAGATVVGLNCSVGPKETLDA
                     LARMRRATSAPLCAQPNAGLPQIIEGRTLYLTTPEYMAVYARRMIESVGLSIIGGCCG
                     TTPSHIRAIKSLVKMLQPTQQPKESLEVEILDARSPVEPSFTPDYSLSPLAAKLSSGV
                     FVKSVEISPPQGTDLEKTLAGARLCAEAGIDCINIPDGPRASARMSPLALAVLFRNDI
                     GIDPIVHLCCRDRNIIGMQADLLGLNALGIHNVLLVTGDPPKLGDYPDATAVFDVDSI
                     GLVQMTQRLNQGSDLAGKAIGKPTSLFIAVGADPGALDFDKEIERLQRKIKAGASCIF
                     TQPVFDMDLFERFQKAVAPLNTPVFVG"
     gene            1827..>2536
                     /locus_tag="PKH07_09360"
     CDS             1827..>2536
                     /locus_tag="PKH07_09360"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNT35191.1"
                     /translation="MAPSSALAIVGGEPLIKDRFPDVFKWPVVTQEDEEAVLEVLRAG
                     SMSGTGVTKQLEVEFAAWQGAKYALGHNNGTAALHAAMFGCGVGVGDEIICPSITYWA
                     SCLPALSLGASVVFADIKLETINVDPADVEHRITDRTKAIVVVHYSGMPVDMDPIMDI
                     AQRHGLKVIEDVSHAHGGLYKGRKVGSIGHVGAFSCMSGKALPWGEAGILVTDDREIY
                     ERALIFGHYERHGAEALLT"
BASE COUNT          618 a          627 c          681 g          610 t
ORIGIN      
        1 accaacaaaa acaggagtat tcagaggagc cacggccttc tgaaagcgct caaacaagtc
       61 catatcaaag acgggctgcg tgaatataca cgaagcaccg gctttgatct tccgttgcag
      121 tcgctcaatc tctttgtcaa agtcaagagc tcctggatcg gcgcccacag caatgaagag
      181 actggtcggt ttgccgatcg cctttcctgc caagtctgac ccttggttca gtcgttgtgt
      241 catctgaacc aatccaatgg agtcaacgtc aaagacagcc gtggcgtccg gatagtctcc
      301 taatttgggt ggatcgcccg tgacgagaag gacattgtga atcccgagtg cattgagtcc
      361 gagaaggtct gcctgcatgc ctatgatgtt cctgtctctg caacacaaat gaacaatcgg
      421 atcgataccg atatcatttc ggaagagtac cgcgagtgcc agagggctca ttcgggcact
      481 tgcacgtggc ccatctggga tattgataca gtcaattcct gcctctgcgc acagccgcgc
      541 accggcaagg gtcttctcga gatcggtgcc ttgcggaggg ctgatctcga cacttttgac
      601 aaacactccg gaagagagct tggcagccag aggggaaaga gaatagtccg gtgtgaaaga
      661 aggctctacg ggagaacgcg cgtcaagtat ctcgacttca agagattcct ttggctgttg
      721 tgttggctga agcatcttga cgagagactt gattgccctg atatgagaag gcgttgtgcc
      781 acaacagcca ccgataatgc tcagtcctac agattcgatc attcgtctcg catagactgc
      841 catgtattca ggagtagtca gatagagagt tctgccttcg atgatctgtg gaagtcctgc
      901 attcggttgt gcgcatagtg gtgctgaggt agctcttctc atccgggcaa gggcatccaa
      961 agtctccttc ggaccgacac tacaattgag accgacaact gtggctccag cctcagtaag
     1021 cgtctttgca atctcctcgg gagttacacc aaagacggtt gtgccactct cagaaacact
     1081 catctgggca acgatgggga tatccgtgat ctctctggca gtttcgaggg caagtcgaat
     1141 ctcctccaag ttggaaaacg tctccaggat gaggagatca acttcggcag ccacaagcgc
     1201 gaaaacgtgc tcgcgaaatg tctccttggc tactgagagt gctatcggtc caaatggttc
     1261 aagactcttg ccgacgggcc caacagagcc agcgacatag gcagtatctc cggcaatact
     1321 tcgagcaagt tccactccct ttcgattgat gagttccaac tgatcctcaa gtccatgatg
     1381 gacaagtttg atgcgattcg caccgaaagt attcgtttcg atgatttcgg cccctgcctt
     1441 cagataggcc tcgtgtacct cggagattaa gttcggagat tgaagattca gtccctcaaa
     1501 gcatctattg atgtaaatgc ctttggagta tagaagtgtt cccatagctc cgtcacaaac
     1561 gatgactccc gaccgaagac gatccataaa aggaactctt tgcatgagaa caacctcctt
     1621 gatgacccag gaatgcccat atcacgtcac ctatcatgat gacgactcag acagatgagg
     1681 gcattccaga gaatccaaac ttattgggaa tgcttacccc tggtcaagta caaaatatgc
     1741 cgagccctgc tgaacggatt tgacatggac gatcttctga ttcaggattt gcagagttgc
     1801 agtagctatc tctgaggagg tttgaaatgg ctccgagttc tgcattggca attgttggtg
     1861 gtgaaccttt gatcaaagac aggtttcctg atgtgttcaa gtggcctgtt gtgacacagg
     1921 aagacgagga ggctgttttg gaggtactcc gcgccggttc catgtccgga acaggcgtga
     1981 ccaagcaact cgaagttgag tttgcggcct ggcagggcgc caagtatgcg ctgggtcaca
     2041 ataacggaac tgctgcactt catgccgcga tgtttgggtg cggagtcgga gttggtgacg
     2101 agattatttg cccaagcata acctattggg catcctgtct gcccgctctc agcctcggcg
     2161 catccgttgt ttttgccgat atcaagttgg agacgatcaa cgttgatcct gcagatgtgg
     2221 agcatcgcat caccgatcga acaaaggcga ttgtggtcgt tcactatagt ggaatgcccg
     2281 ttgatatgga tccgatcatg gatattgcgc aaaggcacgg gctcaaagtc attgaggatg
     2341 tgtcacacgc acatggtgga ctgtacaagg ggcgcaaggt cggaagcatc ggccatgttg
     2401 gagccttttc ctgtatgtcc ggaaaggcct tgccgtgggg agaggctggc atactggtaa
     2461 ccgacgatcg ggaaatctat gagcgggctc tcatcttcgg ccattatgaa cgccatggtg
     2521 cggaagccct gctgac
//
LOCUS       DAOQUC010000509         2535 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_49694_length_2535_cov_2.912500, whole genome shotgun sequence.
ACCESSION   DAOQUC010000509 DAOQUC010000000
VERSION     DAOQUC010000509.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2535
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49694_length_2535_cov_2.912500"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1537)
                     /locus_tag="PKH07_09365"
     CDS             complement(<1..1537)
                     /locus_tag="PKH07_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016695 - oxidoreductase activity, acting
                     on hydrogen as donor [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase, group A"
                     /protein_id="HNT35192.1"
                     /translation="MIQIEANNRTIDAKEGETILSALKRAGISVPTLCHMEGMTPTGA
                     CRICVVEVEGQNSLIPSCAYPVSAGMKIRTHSERALRARKTVVELLLANHPDDCLYCI
                     RNGNCQLQSLAEEFGIRQRRYVGNKNQFNLDVSSPSIVRDPEKCILCGKCVRVCEEVQ
                     GVSAIDFIGRGCRTQIGTAFNQGLNVSSCVNCGQCIMVCPTGALREQSSVKDVIDALQ
                     DPDKYVVVQHAPSVSVTLGEEFGIRPGVDVAGIMTAALRRLGFERVFDTAFGADLTVM
                     EEASELVDRIKNQGTLPMLTSCSPGWVKFVEQFYPEFLANLSTCKSPQQMLAAVIKSY
                     FAEREGLNPEKIFSVSIMPCVAKKFEAERPEMLREGLPDIDAVLTTRELARIIKMRGL
                     DPKSLEPEPPDTPFGMRSTAGKIFGASGGVAEAAIRTAHYLLTGTEAQELKVQAARGL
                     EGIKEFSVSVNGVEINVAVVSGLSNARRVLDAVKQGRKQLHFIEVMTCPGGCIAGGGQ
                     PYQA"
     gene            complement(1558..>2535)
                     /locus_tag="PKH07_09370"
     CDS             complement(1558..>2535)
                     /locus_tag="PKH07_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015311392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HNT35193.1"
                     /translation="FVCGEETALIHSIEGKRGMPRPRPPFPAVKGLFGKPTVINNVET
                     LANVPDVIARGSGWFSAVGTATSKGTKVFALSGMVERTGLVEVAMGMSIRQIIFDIGG
                     GIPNGKRYKAAQIGGPSGGCIPEEHLDLQIDYESLKRFGAIMGSGGLVVMDEGACMVD
                     VAKFFMDFIQRESCGKCIPCREGTRRLLEILERITKGRHGEQKTDALLRFQGVMYLDR
                     LARVIQDTSLCGLGQTAPNPVLSTLRWFRDEYEAHIYERRCPAGVCRDLLHYRIDAEK
                     CKGCTLCLKKCPVQAIMGAPKSPHYIIADKCIGCGNCQEVCRFGAVVVE"
BASE COUNT          591 a          779 c          588 g          577 t
ORIGIN      
        1 cggcctgata tggctgacca ccaccggcaa tacatccccc cggacaggtc atcacctcga
       61 tgaagtgcag ttgctttctt ccctgtttga cggcgtccag aactcgcctg gcgtttgata
      121 gaccactgac aacggcaaca ttgatctcca caccgtttac cgaaacgctg aattccttga
      181 tgccttccaa acctcgggca gcctgaacct tgagttcttg agcctctgtg ccggtcagca
      241 gatagtgtgc cgttcgaatc gctgcttccg ccactccacc tgaagcaccg aatatcttcc
      301 ccgccgtact tctcataccg aagggagtat cgggaggctc tggctcaaga ctcttcggat
      361 ccaaaccccg cattttgata attcgagcca attctcgcgt cgtcaacacg gcatcaatat
      421 caggcagacc ctcgcgaagc atctctggac gctcggcctc gaatttcttg gcaacgcacg
      481 gcatgatgga aacactgaat atcttctcgg gattcagccc ctcacgctca gcaaaatagc
      541 tcttgatgac ggcagcgagc atctgctggg ggctcttgca ggtggaaaga ttcgccagaa
      601 actcggggta gaactgttcg acaaacttga cccatccagg agagcagctg gtcaacatcg
      661 gaagggttcc ctggttcttg attcggtcaa caagttcgga cgcttcttcc atcaccgtca
      721 gatcggcgcc aaaggccgtg tcaaagacac gttcgaatcc aagccttctc aatgctgcgg
      781 tcatgatccc cgccacgtct acacccggcc tgattccgaa ctcttctccc aatgtcaccg
      841 aaacactcgg agcgtgctga accacaacat acttatcggg atcctgaagc gcgtcaatga
      901 catccttcac actgctctgc tcccgaagcg caccagttgg acagaccatg atgcactgac
      961 cacagttgac acacgatgaa acattcagtc cctggttaaa ggcggttcca atctgcgttc
     1021 tgcaacctct gccgatgaaa tcaatggcag aaacgccctg cacctcttca caaacccgaa
     1081 cgcacttccc acagagaatg catttctccg gatctcgaac gatcgaagga ctcgaaacat
     1141 cgagattgaa ctggttcttg ttgccaacat aacgccgctg acggattcca aactcctcag
     1201 ccagtgactg tagttggcag ttgccgttgc gtatacagta cagacagtcg tccggatgat
     1261 ttgccagcaa aagttccaca acggtctttc gagctctcaa tgcccgttcg gagtgcgtcc
     1321 taatcttcat cccggcactg accggatatg cacagctggg aatcaggctg ttctgtcctt
     1381 ctacctcaac cacgcagatg cgacaggctc ccgtaggagt cattccctcc atgtgacaca
     1441 aagtcggcac actgataccg gctctcttga gggcactgag aattgtctca ccctctttgg
     1501 catcaatggt tctgttgttg gcttcaatct gaatcatgac agtatctatc ctttgtatca
     1561 ttcaacgaca acggcaccaa accgacacac ttcctggcag ttgccacagc cgatacactt
     1621 gtccgcaatg atatagtgag gactcttggg cgctcccatg attgcctgaa ctgggcattt
     1681 cttcaggcaa agagtacacc ctttgcactt ttcggcatca atccggtagt gaaggagatc
     1741 cctgcaaaca cccgcgggac agcgacgctc gtagatgtgc gcctcgtatt catcgcgaaa
     1801 ccagcgcagt gtgctcaaca ctgggtttgg agcggtctga cccagtccac agagactcgt
     1861 gtcctgaatc acccgtgcca gacggtccag atacatcact ccctggaatc tcagcagcgc
     1921 gtcagtcttc tgctctccat gacgaccttt ggtgatgcgt tcgagaatct caaggaggcg
     1981 tcgagttccc tcacgacacg gaatgcactt gccgcagctc tcacgctgga tgaagtccat
     2041 aaagaacttg gctacatcca ccatgcacgc cccctcgtcc attaccacaa ggccccctga
     2101 tcccatgatt gcaccaaatc gcttcaggga ttcgtagtca atctgcaagt ccagatgctc
     2161 ctcaggaatg cagccacctg aaggcccgcc aatctgcgct gccttgtaac gcttgccgtt
     2221 gggaatgcca ccgccgatgt caaagataat ctgccggatg ctcataccca tggcgacttc
     2281 cacgagacct gtccgctcaa ccattcccga caaggcaaag actttcgtcc ccttactggt
     2341 ggcagttcca accgcactga accaacctga tccgcgtgcg atgacgtcgg gcacattagc
     2401 aagagtttcc acgttgttga taaccgtcgg cttaccgaaa aggcctttca cggctggaaa
     2461 aggtggtcgt ggccgaggca tcccgcgctt gccttcgatg ctgtggatca gtgctgtctc
     2521 ctcaccgcag acaaa
//
LOCUS       DAOQUC010000510         2535 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_49695_length_2535_cov_2.879839, whole genome shotgun sequence.
ACCESSION   DAOQUC010000510 DAOQUC010000000
VERSION     DAOQUC010000510.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2535
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49695_length_2535_cov_2.879839"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..544
                     /locus_tag="PKH07_09375"
     CDS             <1..544
                     /locus_tag="PKH07_09375"
                     /inference="COORDINATES: protein motif:HMM:NF021903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HNT35194.1"
                     /translation="RAVQPGERLNIMGPLGKGFALPEADQPALLVAGGVGIAPLHLLA
                     RRLSEQGRPVTVLWGGASREAVDLGMETSWPLLEATMDGSIGYQGMVTDLLVRFLEQE
                     GNIPWVGSCGPEPMLRQVARVCASKGVAVQLCFEARMACGWGVCLGCAVAVKDGQGSV
                     SYKRVCSDGPVFWGHELTEW"
     gene            612..1658
                     /locus_tag="PKH07_09380"
     CDS             612..1658
                     /locus_tag="PKH07_09380"
                     /inference="COORDINATES: protein motif:HMM:NF015087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HNT35195.1"
                     /translation="MKTVSIGKRLLGEGQPCYIIAEAGKNHNGDVELARELVRKAAEA
                     GVDAVKFQSIDTGRLMIRSGLRPAYFDETVGSKETEFDITKRLELPDSAHPELADLAR
                     SLGIDFISTPEDAGHADLLCDLKVPLLKVSSLNIDNVPLLRHMASKGLPMVISTGMAT
                     LGEVEQALDVVLSNGCPGVVLLQCTSNYPALPEDVNLRAMVTMRQTFGVPTGFSDHTM
                     DDTMALGAVALGASVYEKHFTLDRNMEGPDHRLSADPSEMKAIVERIRLLEKGLGNPI
                     KKPTQTELEMKMFKRRSIVAARAVKQGTVLTADDLDCKCPGTGLSPRFFDILIGRKAQ
                     RDLPQDSILTWDDV"
     gene            1712..2293
                     /locus_tag="PKH07_09385"
     CDS             1712..2293
                     /locus_tag="PKH07_09385"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNT35196.1"
                     /translation="MKRNCLVAVLLACCVLSCGGRKAPEDLSTIVLTHETIHAAAAAG
                     DEREARGFIARGVDVDTRDRNECTPMHYAAEGGHAATVRALLSELADPQAKDSLGWTP
                     LHRAAAEGHLDVVKMLVPRCRSIDEETDSSETALHFAAEKGHFEVVKYLLEQGAVVNP
                     KNLAGETPLRLASNNHHENVVKHLLENGADFND"
     gene            2346..>2535
                     /locus_tag="PKH07_09390"
     CDS             2346..>2535
                     /locus_tag="PKH07_09390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35197.1"
                     /translation="MRKLFGVVFFVTLFVFCLGCSGKPKETAEPQQQGAVMREEAVAQ
                     EEAQEQQAQQQEAQPAAEK"
BASE COUNT          580 a          591 c          809 g          555 t
ORIGIN      
        1 gcgcgcggtt cagcctggag agcgtctgaa catcatgggg cctttgggca agggttttgc
       61 acttcccgag gcagatcaac cggcgctgct cgttgctggt ggcgtgggca ttgcgccgct
      121 tcacctgctg gcaaggcgtt tgtcagagca gggacggccg gtaacggttc tttggggtgg
      181 cgcgagtcgg gaagcggttg acctcggcat ggaaacatcc tggccgttgc tcgaagccac
      241 gatggatgga agtatcggat atcagggcat ggtgaccgat ctcctcgtca ggtttctcga
      301 acaggaggga aacattcctt gggtgggttc ctgtggcccg gaaccgatgc ttcggcaggt
      361 tgcccgagtc tgcgcatcca agggtgttgc ggtgcagctt tgtttcgaag caaggatggc
      421 gtgtgggtgg ggagtctgtc tgggctgcgc tgttgcggtg aaagacggac agggatcggt
      481 atcttacaag cgcgtttgtt cggatggccc tgttttttgg ggacatgagt tgacagaatg
      541 gtagaataga tttggagtag aagatcggtc tgcatgacat cggttgcagg cgttcaaaaa
      601 ggatgggatc aatgaagacg gtatcaattg gaaagcgact tcttggagaa ggacagccct
      661 gctacatcat cgccgaggcc ggaaagaacc acaatggtga tgtcgaactc gcacgggaac
      721 tggtgcgcaa agcagccgag gctggagtgg atgcggtcaa gttccagtcc attgacaccg
      781 ggcgactcat gatacgaagt ggtctgagac cggcctattt cgatgagact gtgggatcca
      841 aggagacgga attcgacatc acaaagaggc tggagcttcc ggattccgcc catccggagt
      901 tggccgatct ggcacggagt cttggcattg atttcatctc gactccggag gatgctggac
      961 acgcggactt gctgtgtgat ctgaaggtgc ccttgctgaa ggtttcatct ttgaacattg
     1021 acaatgttcc cttgttacga cacatggcgt ccaagggttt gccgatggtc atttccaccg
     1081 gcatggcaac gctgggcgaa gtggaacagg ctctggatgt ggtgctctcc aatggatgcc
     1141 caggcgtggt cttgctgcaa tgcacatcga actatccggc gttgccggaa gatgtgaatc
     1201 tgcgtgccat ggtgactatg cgacagacgt ttggagtccc caccggcttc tcggatcaca
     1261 ccatggatga tacgatggct ctaggtgccg tggcacttgg agccagtgtg tatgagaaac
     1321 attttacact agacagaaac atggaaggcc ccgatcatcg tctgtccgcc gacccatcgg
     1381 agatgaaggc cattgttgag agaatacgcc tgctggagaa gggactcgga aatccgatca
     1441 aaaaaccaac gcaaaccgag ttggagatga agatgttcaa acgaagaagc atcgtcgcag
     1501 cacgtgctgt caaacaggga acggtactga ctgctgacga tctggactgc aaatgtccag
     1561 gaactgggct ttcccctcgc ttttttgata tccttatcgg aagaaaggca cagagggatc
     1621 ttccccagga tagtatacta acctgggatg atgtctagac tgcttaacga cgcggagccg
     1681 gtgcgtcggg aatacacggg agggacttct gatgaaaagg aactgtcttg ttgcagtgtt
     1741 gctggcgtgt tgtgtgctat cctgtggggg aaggaaggcc cctgaggatc tgagcaccat
     1801 tgttctgact cacgaaacga tccatgccgc agccgcggca ggtgatgaac gggaagcacg
     1861 cgggtttatt gcccgcgggg tggatgtgga cacgcgagac aggaatgagt gtacaccgat
     1921 gcactacgct gcagagggtg gccatgcggc cacagttcga gcgcttcttt cggaactggc
     1981 cgatccccag gccaaagaca gtctcggatg gacgcccctg catcgcgctg ccgcagaagg
     2041 ccatctcgat gtggtgaaga tgctggttcc ccgatgccga agcattgatg aggaaacaga
     2101 ttcgagtgag acagcgcttc acttcgctgc agagaaaggt cacttcgagg tggtcaagta
     2161 tctgctcgaa caaggtgctg ttgtgaaccc caagaatctc gcaggggaga cgccgctgcg
     2221 gttggcttcc aacaaccatc atgaaaatgt cgtcaagcac ctgctggaga atggagccga
     2281 ttttaacgac tgattgtctc tccagggttg ggtgatactt gggcaggaga aatgaggagg
     2341 tagagatgcg aaaattgttt ggtgttgtgt tctttgtgac tttgtttgtc ttctgtttgg
     2401 gctgctcggg aaagccaaag gaaaccgcgg aaccccaaca acagggagcg gtcatgagag
     2461 aagaagccgt cgcccaggag gaggctcaag agcagcaggc acagcagcag gaggctcagc
     2521 ccgctgcaga aaagc
//
LOCUS       DAOQUC010000511         2531 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_49820_length_2531_cov_2.985864, whole genome shotgun sequence.
ACCESSION   DAOQUC010000511 DAOQUC010000000
VERSION     DAOQUC010000511.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2531
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49820_length_2531_cov_2.985864"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2531
                     /gene="uvrA"
                     /locus_tag="PKH07_09395"
     CDS             <1..>2531
                     /gene="uvrA"
                     /locus_tag="PKH07_09395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HNT35198.1"
                     /translation="DNVVLILGFPLKIPKTLPVDEAVGYVLQQGFTRVWYQGSIRPIK
                     KGEWYPTQGVEFLVVVDRVALNDQSRQRVVDSIETCMKFGGGLVHIIRPDGTLDKHSE
                     HLHCPFCDIEYSAPHANLFSFNSPLGACEACKGFGRIITVDPAKVIPDPQKSLAEGAV
                     KPWTTDSYSECQQDLLRFCRKQRIPINLPYRGLSESQRQMIYCGKGDFYGVKGFFEWL
                     ESKSYKMHIRVLLSKYRAYIPCPVCHGTRLRDEASWYRLGDRTITEISSLPILQARQF
                     FAALAVEQSRDKATEILVEEIANRLQYLVDIGLDYLTLDRQSRTLSGGEAQRVNLTRA
                     LGGSLVETLYVLDEPSIGLHPRDTDRLITLLYHLRDRGNTVIVVEHDPSLIRAADFVV
                     DLGPGAGSAGGGIVCAGSVGEVIDCPESVTGKHLRLSETERSPQIRPRVIGQENSIRI
                     LGARAHNLKGIDVTIPLGGLVCVTGVSGSGKSSLIQDVLWEALKTERFSELETGPTFD
                     RIETRGDFHGCAYLDQSPAAATPRMNAATAVKAFDGIRKCFAETEISRSRGYSAGTFS
                     FNTPGGRCDVCEGAGHEVVEMQFLSDVSLTCPECGGTRYKPEIRDVLWRGRSIVDVLS
                     MTVSDARSLFHDVPSVDRPLSVLETVGLGYITLGQPSNTLSQGENQRLSLARDIFLYG
                     RQQPMLFILDEPTTGLHLADIQVLLTAFEQMLEQGHSLLVVEHNLEVIRNAHWIIDLG
                     PEGGGLGGNIVVAGTPEQVMACSKSHTGRFLRSYVRGKGLLEKPYNAPSSSRDRPSAR
                     EYLEIFGAHEHNLHNVSVRIPRNKFVVVTGVSGSGKSSLVFDIVF"
BASE COUNT          583 a          636 c          709 g          603 t
ORIGIN      
        1 cggacaatgt tgttctgatt cttggatttc ctctgaagat accgaagaca ctcccggttg
       61 acgaagcggt gggatatgtt cttcagcagg ggtttacgag agtctggtat caaggcagca
      121 tccggccgat caagaagggc gaatggtatc ctacacaggg tgtggagttc ctcgttgttg
      181 ttgacagggt cgcactgaat gatcagtccc gacagagagt cgtggattcg attgaaacgt
      241 gcatgaagtt tggaggcggt cttgtccata tcatcaggcc agatggcact ctggataagc
      301 actctgaaca tcttcactgt ccgttttgtg atattgaata ctctgcaccc cacgcgaatc
      361 tcttttcttt caacagtcca ctcggcgcct gtgaggcctg caaagggttt ggccgcatca
      421 tcacggtcga tccggccaag gtaatcccgg atccgcaaaa gtcgctcgct gaaggagctg
      481 tcaaaccttg gacaacagac tcctacagtg agtgccagca ggatctcctg cggttttgca
      541 gaaaacagag aatcccgatc aacctcccat atcgaggcct ctccgaatcg cagaggcaga
      601 tgatctactg cggaaaaggt gacttctatg gagtgaaggg attctttgag tggcttgaat
      661 caaaatcgta taagatgcac atccgtgtac tgctatcgaa atatcgtgcc tatattccgt
      721 gccccgtgtg ccatggaact cgtcttcgag acgaggcttc ctggtaccgc ttgggggaca
      781 ggacgatcac ggagatcagc tcccttccca tcttgcaggc caggcagttt ttcgcagcac
      841 ttgcggtgga acagtccaga gacaaggcaa cagagatcct tgtcgaagag attgcaaatc
      901 gccttcagta cctcgtggac ataggtttgg actacctcac tctcgatcgg cagtccagaa
      961 cgctctcggg gggtgaagcc cagagagtga atctgactcg cgctttgggc ggaagccttg
     1021 tcgaaacatt gtatgtcttg gatgagccga gcattggact gcacccgaga gacaccgatc
     1081 gcctgatcac gctgctctat catctgcgag atcgtggtaa cacagtcatc gtggtggagc
     1141 acgacccttc tctgatacgt gcagcggact tcgttgttga tctcggtccg ggtgctggaa
     1201 gtgctggcgg aggaattgtc tgtgcaggat cggtcggaga ggttatcgat tgtcctgagt
     1261 cagttacagg aaagcacctt cggcttagcg agacagagcg atcgcctcag attcgtccac
     1321 gcgttattgg tcaagagaac tccatcagaa tccttggcgc acgagcacat aatctgaagg
     1381 ggattgatgt gacaataccc ttggggggac tggtgtgcgt caccggagtc tccggttctg
     1441 gcaagagttc cctgatacag gatgttctct gggaagctct caagactgaa aggttctccg
     1501 agttggagac agggcctacc ttcgaccgga tcgagacaag gggcgacttt catggatgcg
     1561 cctatctgga tcagtcaccc gcagctgcca caccacgcat gaatgctgcc actgccgtaa
     1621 aggcttttga cgggattcgc aagtgctttg ccgaaacgga gatttccagg agccgcggat
     1681 acagtgccgg aacattctcc ttcaataccc ctggcggacg ttgtgacgtg tgcgagggtg
     1741 ctggtcatga agttgtcgag atgcagtttc tgtcggatgt ctccctgacc tgtcccgagt
     1801 gtggaggaac aagatacaag cctgagatac gagatgtcct ttggagaggg agaagcattg
     1861 ttgacgttct atccatgaca gtgagcgacg cgagaagtct ctttcacgat gtccccagcg
     1921 ttgatagacc actgtcggtc ttggagacgg tcggtcttgg gtacatcaca ttgggacaac
     1981 cgtcgaacac tctctctcaa ggcgagaacc agaggctgag tcttgcacga gacatctttc
     2041 tctacggccg acaacagcca atgctgttca ttctggatga accaacgact ggactccatc
     2101 tcgccgatat tcaggttctc ttgactgcct ttgagcagat gctcgagcag gggcattctc
     2161 tgcttgtagt cgaacacaat ctggaagtaa tccgaaatgc gcactggata atagaccttg
     2221 gccctgaagg tggaggcctg ggcggaaata tcgtggtcgc aggaactccg gagcaggtga
     2281 tggcgtgcag caagtcgcac accggcagat tccttcgcag ttacgtccgc ggcaagggtc
     2341 tcctcgaaaa gccttacaac gctccttcgt cctcccgaga caggccatct gcgagagagt
     2401 atctggagat atttggcgct catgaacaca atctccacaa tgtcagcgta agaatcccac
     2461 gcaacaaatt tgttgttgtg acaggagtca gtggttcggg aaagtcctcg ctggtgttcg
     2521 acattgtctt t
//
LOCUS       DAOQUC010000512         2531 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_49823_length_2531_cov_2.925687, whole genome shotgun sequence.
ACCESSION   DAOQUC010000512 DAOQUC010000000
VERSION     DAOQUC010000512.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2531
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49823_length_2531_cov_2.925687"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..961
                     /locus_tag="PKH07_09400"
     CDS             <1..961
                     /locus_tag="PKH07_09400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35199.1"
                     /translation="LWNQSRGIGGSIYMHTGGMAGRYSDDEGITWSEIQDIPFPNGPL
                     DDPQGKIPPEWIVWQIPTRDLKDRFYVGFSHWVNKTAGYKHPPGRLGWTWLESVVEFM
                     RFENIDNDPEPKDIQVVFRSYGDEALRVPHWRDPLVSVAQEPSLVRLPDQRLFCTMRT
                     NTGYIWYSLSEDDGEHWCMPRPLLDKDFGRPLLQPVGCCPIYDISAGRYLLLYHNNRG
                     NITPLPEEPEETACPRNPAYFALGEFRPKAHQPIWFSKPKVFINIRTEGVDGKPNPTQ
                     SIACYTSFTNVQGNRVLWYPDRKYFLLGKRVRDEFLADMQVPD"
     gene            1086..1385
                     /locus_tag="PKH07_09405"
     CDS             1086..1385
                     /locus_tag="PKH07_09405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35200.1"
                     /translation="MPKERRQSVKPMREQAESVPATPQAVKPDERFKLWEYALVNLIF
                     WAFCMVQYVVVRVVTRESLGLMFFFSSLAIGFSVVSVLSWCFDAFYPEKDTEDTC"
     gene            1591..2379
                     /locus_tag="PKH07_09410"
     CDS             1591..2379
                     /locus_tag="PKH07_09410"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HNT35201.1"
                     /translation="MKVVTGKTMADLDRRAIQEHGIPSLQLMEKAGYGAMEILLRRFE
                     PCPESRIVIIAGCGNNGGDGFVLARLLQEKGFVPTVWIASTPDRLKGDALENYKRYSA
                     MAKPVSLLTGEKDLDVLADSCACADVIVDALLGTGFRGQVQSPYLEIIETMNKSFAKV
                     LAMDVPSGLNADDGTVSGACVKADVTATMQLPKLGLLVPPGVDFAGDIEVIPLEFPKE
                     LLVNDGHSPEFLTERDVASLLPIRERSAHKGTAGHLLILAGSQV"
BASE COUNT          589 a          638 c          702 g          602 t
ORIGIN      
        1 gctctggaat cagagtcgag gcattggcgg aagcatctat atgcacacag ggggcatggc
       61 agggcgctac agtgacgatg aaggcataac gtggtctgag atacaggata tcccctttcc
      121 caatgggcct cttgatgatc cccagggcaa gattcctccg gaatggatcg tctggcagat
      181 tccgacgcgg gacttgaaag accgtttcta tgttgggttt tcgcattggg tcaacaagac
      241 agcgggctac aaacacccgc cgggaaggct gggttggact tggcttgagt ccgtcgtcga
      301 gtttatgcga ttcgagaaca ttgacaacga tcctgaacca aaagacatac aggttgtctt
      361 ccgctcatac ggtgatgagg cgcttcgggt tcctcattgg cgggatccat tggtcagtgt
      421 ggcacaggaa ccaagcctgg ttcgactgcc tgaccagcgc cttttctgca cgatgcgaac
      481 caataccggg tacatttggt acagcctttc cgaagacgat ggggagcact ggtgcatgcc
      541 aaggccgctc ctggacaagg attttggcag accgctactt cagccggtcg gctgttgtcc
      601 catctacgac atatcagcgg gcagatattt gcttctctac cacaacaacc gcgggaatat
      661 cactcctctt ccagaagaac ccgaagagac tgcttgtcca agaaacccag cctacttcgc
      721 cttgggagag ttccgaccaa aagcccatca gccgatctgg ttcagcaaac ccaaagtttt
      781 catcaatatc agaactgagg gagtggacgg gaaacccaac ccgacacagt ccattgcctg
      841 ttacaccagt ttcaccaatg ttcagggaaa cagggttctc tggtacccgg atcgcaagta
      901 ttttctgctc ggcaagcggg tccgagatga attcctggcc gacatgcagg ttccagactg
      961 aaaaaccttg acagctgtga acgatttcac caatatatat tgagacgtgg ttgatatagc
     1021 gtgcgagtaa tcgtacgtag aaaggacgat tctaagtttc agttcctatg ggaacaagag
     1081 ataggatgcc taaagagaga agacagtcag taaaaccaat gcgtgagcaa gcggagtccg
     1141 taccggcaac tccgcaggct gtaaagcccg atgagcgttt caaactgtgg gagtacgctc
     1201 tggtcaatct cattttttgg gctttttgca tggttcaata cgtggtcgtt cgggttgtta
     1261 cccgggaatc cctcggcttg atgtttttct tttcatcgtt ggccataggg ttctcagttg
     1321 tttcggtatt gtcatggtgt tttgatgcgt tctatccgga aaaggatacc gaagacacct
     1381 gttgatccga aatccgcaac gcatgcttct ctgcacgttt cctatctttg tttgaggaac
     1441 cgactggttc gtcgtggaat tcaactgtgt ccctgtgcgc ttgcatgagg gctgtgctct
     1501 atgcagagag ttcgtatatg gagcgactgg cacttggaga ggtcatcgcc cacacgcccg
     1561 caggtgccaa cctgctctag aggaagattg atgaaagtcg ttactggaaa gaccatggcc
     1621 gacctggatc gaagagccat ccaggaacat gggattccca gccttcaact gatggagaag
     1681 gcgggatacg gtgccatgga aatcctcctc agaagattcg aaccgtgtcc ggaaagtcga
     1741 attgtcatca ttgccggctg cggcaacaac ggtggagatg gcttcgtcct cgcccgcctt
     1801 ctgcaggaga aaggctttgt tcccactgta tggattgctt caactcccga ccgactcaag
     1861 ggagacgcac tcgagaacta caaacggtac agcgcaatgg caaagcccgt ttcactgctc
     1921 acgggtgaaa aggatttaga cgtcttggcg gattcatgcg cttgtgctga cgtgatcgtg
     1981 gatgcgctgc tgggcaccgg attccggggt caggtgcagt cgccctatct cgagattatc
     2041 gagaccatga ataagtcctt cgcgaaagtg ctcgccatgg acgttccctc cggcctgaat
     2101 gccgatgacg ggacagtttc tggcgcctgc gttaaggccg atgtgaccgc aacaatgcag
     2161 ttgccaaaac ttgggttgct tgtgccacct ggggttgact tcgcgggtga tatcgaagtt
     2221 attcctctgg aatttccaaa ggaactgctt gtgaatgatg gccattctcc agagtttctg
     2281 actgaaagag acgtggcttc tctgctaccg atccgagaac gttccgctca caaaggcact
     2341 gctggccatc tgctgattct tgccggatcg caggtctgac tggagcggcg gcattggctg
     2401 ccgagagtgc tgtaaggagt ggtgcaggac tggtcacatt gggcgttccg gaaagcctga
     2461 atccgatatt ggagaccaag gtcaccgagc caatgactct gcccctgccc gagacaggcg
     2521 atcacacgct g
//
LOCUS       DAOQUC010000513         2528 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_49904_length_2528_cov_3.541448, whole genome shotgun sequence.
ACCESSION   DAOQUC010000513 DAOQUC010000000
VERSION     DAOQUC010000513.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2528
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49904_length_2528_cov_3.541448"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..825)
                     /locus_tag="PKH07_09415"
     CDS             complement(<1..825)
                     /locus_tag="PKH07_09415"
                     /inference="COORDINATES: protein motif:HMM:NF020700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35202.1"
                     /translation="MKRNSLILVLLVLCCLVSCARKDGYYRIEKPRIFRGEWFDYYNR
                     ALWLIQEGKHQQAEGDLRSAIADLDRDTWEERTYGVHFIEYFAHRELGILLFNQGRLD
                     ESLVELETSLKQADSGRTKFYLNRVHRELISSKGLDATEPSITLETPDDNSTVPDYSV
                     VLKGSAEDDTLVFAVRINGEPVFIELAEPTVDFERVVPLQSGSNEIELEVEDIVGRKT
                     KRTLRVGADREPPLLLVHQTELRRGSFHLVATLEDPGGLRSARFGSSVLELEGHASY"
     gene            complement(901..>2528)
                     /locus_tag="PKH07_09420"
     CDS             complement(901..>2528)
                     /locus_tag="PKH07_09420"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNT35203.1"
                     /translation="LRLAREHIIKEYGESCLIWATYLMYGHPDRCLFAAHQEDSPACS
                     EPLEQVRSTTPAVKAAASGSGFRKSWLAVAASVVVVVCLGSYLFTNSHNSGDPRSASE
                     ILQQGYGAVLAGDYLSAMACFDSIALRKHSLVGKTGLAYVALKQERFDDATKLSDEVL
                     EVDPKASFALTIKGAVLLRQNQPQTAKPLLEAGSSGSLGFSWQRAFALSLLAQVLAQE
                     SDEPGAIAKAEEAVQAAPEFPDALKTLADLYVKAGRNADALRLYEKVSASSPNDRWIS
                     FRTYELKRREKMEQEKLSQERVNFLLQELSKDTPLRNVEEGDRWTSRVVGMTIVPPEI
                     HGSEVSQQGEDEYLVYCLSDLLAKSGRFRVVDRSLLDSVLMELNLSNDGLANAQNAVR
                     VGELLSARYVIAGTLFYLPGGIIKGKFRVIDTETSEIVAYPSAESDSGFDSLIDSLGS
                     VLLESIVSRNPIQGKVVPSEGSSTELKVNVGRAHGVKEGMVVEILHADTSERLSEGRV
                     VSVDDKTAQIRVESGTDGIAVGSRIRESQASES"
BASE COUNT          615 a          724 c          599 g          590 t
ORIGIN      
        1 gtagcttgca tggccttcga gttcaagaac actgctccca aaccttgcag aacgaagccc
       61 accggggtct tcaagtgttg cgacaagatg aaacgaccct cttctcagtt ccgtctgatg
      121 taccagcagc agaggtggct ctcggtcagc tccgactcgc agtgtcctct ttgtctttct
      181 tccgacgatg tcttccactt ccaactctat ttcgttcgaa ccggactgta agggaaccac
      241 cctctcaaaa tccacggtcg gctccgcaag ttcgataaac accggctctc cgttgatgcg
      301 tactgcaaac acgagtgtgt cgtcctcagc tgaacctttc aacacgaccg aatagtccgg
      361 aaccgtagag ttgtcgtctg gagtctccaa agtgatcgaa ggctctgttg cgtcgagtcc
      421 tttcgaggag ataagttctc ggtgcaccct gttgagatag aattttgtcc tgccggaatc
      481 agcctgtttc aaggacgtct ccaactcaac gagagattcg tccagccttc cttgattgaa
      541 cagcaggatt cccaactccc gatgtgcaaa gtattcgatg aaatgaacgc cataggtgcg
      601 ctcttcccaa gtgtctctat ccagatcagc aatagcagat cgaagatccc cctccgcctg
      661 ctgatgcttc ccctcttgaa tcagccagag cgctctgttg tagtagtcaa accattcacc
      721 ccggaaaatg cgaggcttct caatacgata gtagccatcc ttacgcgcac aactgacgag
      781 acaacagaga acgagaagta caaggatcag agagttcctc ttcatactcg ctctgtacat
      841 ttttgtgcag tgcatatctc tactcaacac ttcggccttc tacgaatctg gaacaagggc
      901 tcaggactcc gaggcttgac tctcccttat ccggcttcca accgcaatcc catcagtgcc
      961 tgactccacg cggatttgcg cagtcttgtc atcaacactc accacgcgtc cttctgagag
     1021 ccgttcgctt gtgtccgcat ggagtatctc aacgaccatt ccttctttga ctccatgggc
     1081 ccgtccgaca ttaactttca gctcagtaga agatccctcg gatggaacaa ccttgccctg
     1141 aatgggattt ctggaaacaa tgctctcaag gagaacagat ccaagagaat caatcaatga
     1201 gtcaaatccg ctatcggact ccgcagatgg ataggcgacg atctcactgg tctccgtatc
     1261 aatgacgcga aactttcctt tgatgattcc cccgggaaga tagaacagag ttcccgcgat
     1321 gacatacctt gcggacagca actcacccac tcgaaccgca ttctgtgcat tcgccaaacc
     1381 gtcattgctc aagttgagtt ccatgagtac ggaatccagg agtgagcgat caacaacgcg
     1441 gaaacggccg gactttgcca agagatcgga cagacagtaa acaaggtatt catcctctcc
     1501 ctgctgagag acctcagaac catgtatttc agggggaaca atcgtcatgc caacaaccct
     1561 ggaagtccat cggtctcctt cttccacatt tctaagtggc gtgtctttgg agagttcctg
     1621 aagaaggaag ttgactcgct cctgagagag tttctcctgc tccatcttct cacgtcgttt
     1681 cagttcatag gttctgaagg aaatccatcg atcgtttgga ctgcttgcgc tcactttctc
     1741 atagaggcgc agggcatcag cattccgacc cgctttgaca tagagatccg ccagtgtttt
     1801 gagagcatca ggaaactcgg gcgctgcttg gactgcttct tctgccttcg cgatcgcccc
     1861 aggttcatct gattcctgag ccaatacctg agcaagcagc gacaacgcaa aggcacgctg
     1921 ccaactaaag cccaaagaac ccgaagatcc cgcctcaaga agcggcttgg cagtctgcgg
     1981 ttggttctgg cgaagtagta ccgcgccctt gatggtcagg gcaaacgaag ccttgggatc
     2041 cacctccagt acctcatcag acagtttcgt ggcatcgtca aagcgttcct gcttgagtgc
     2101 gacgtaggcg agacccgtct ttccgacaag gctgtgcttt ctgagggcaa tactgtcgaa
     2161 acaggccatc gctgaaagat agtctccggc aagaaccgct ccatagccct gctgcaatat
     2221 ctctgatgcc gaacgggggt ctcctgagtt gtgagagttg gtgaacagat aggacccaag
     2281 acacactacg acaacgacac tggcagcaac agcaagccat gacttcctga agccactgcc
     2341 gcttgccgcg gccttcacgg ccggagtggt ggaacgtacc tgttccagag gctcggaaca
     2401 agcaggcgag tcttcctgat gcgcagcaaa caaacagcgg tctggatgac catacataag
     2461 ataggttgcc cagatgagac aactctcacc gtactccttg atgatgtgtt cgcgagccag
     2521 cctcaagg
//
LOCUS       DAOQUC010000514         2528 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_49930_length_2528_cov_2.253134, whole genome shotgun sequence.
ACCESSION   DAOQUC010000514 DAOQUC010000000
VERSION     DAOQUC010000514.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2528
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49930_length_2528_cov_2.253134"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..992)
                     /locus_tag="PKH07_09425"
     CDS             complement(<1..992)
                     /locus_tag="PKH07_09425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35204.1"
                     /translation="MKTTKLSVFVLFSMCIMFGQYGFAQVDQNLRVDDWNLSLLNKGW
                     KADQMTIQGEYDAAPGTTNPMIQGMTFNFYDGIWGGGLCTDTLISPYFRQGRGSKLEY
                     KNGPEQGAVLLNIGGSSRSRFNFSMRDTYAGARGVTPVQVELEMFPVAGYPFVYFNDT
                     LTILNQLKDPYGFRGSKFNLPVSPVLDEGDGDEVFVDSLIINRNLFAVGKDKCRIAAR
                     IPTLPFFNPGPVEQIEFDLPFGPTTVTTPIGWLRANSNGTILSYNGSLGAIFITNGLP
                     IPAWANGAEIEITLNIVRRTLSIYVTKMTLWDRILNAPNGDNVVNPYDINLSFI"
     gene            complement(1186..2172)
                     /locus_tag="PKH07_09430"
     CDS             complement(1186..2172)
                     /locus_tag="PKH07_09430"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNT35205.1"
                     /translation="MRLSRTEIVLLAVLGVVVLVGLARVLPDSSSRNGLVSTKGTLGE
                     KQPETHAEKDASPRGSQDTDRPNGDNLDLQDANRSQGAESSEKPASSSVSPLAGSQPK
                     KARNIELPAGRISADEALASLCDQLGLELFRSGNANLKAEVQVDDPTPSAILDAVARA
                     ARVPLAGNLDDLDQGYRHQIHFTPLALYVGDNRDVVKALEQLSQGSPESRTDAIKDLG
                     DIAFEQGENLESVLSHNLQTDFNEEVRAEAAVALEHAQSGESIDALVAALSDSSVLVR
                     EHARTTLGLVGTDEVLQKLKQAYPSIEDNEIRKIFDQILEERFDQPIRIEGL"
BASE COUNT          626 a          669 c          594 g          639 t
ORIGIN      
        1 tcaataaagc tcagattgat atcataggga ttgaccacgt tgtccccatt gggtgcatta
       61 agtatcctgt cccacagagt catcttggtt acatagatgg atagcgttct tctaacaatg
      121 ttcaaagtga tctctatctc ggctccattc gcccaagcgg ggatgggcag accgttggta
      181 ataaagatcg ctccgagaga accgttgtaa ctcagaatgg ttccgttgct gttggctctc
      241 agccaaccga tgggagttgt gacagttgtt gggccgaaag gaagatcgaa ctcaatctgt
      301 tcaaccggtc ctggattgaa gaacggcagc gtgggaatgc gagctgcaat tcgacacttg
      361 tccttaccga cggcaaagag attgcgattg atgatcaaag aatcaacaaa cacttcgtca
      421 ccatcgcctt catcaagaac tggactgacg ggcaggttga acttagaacc ccggaagcca
      481 tagggatcct tcaactggtt cagaatcgtc agggtgtcat tgaaatacac gaaggggtat
      541 ccagccacag gaaacatttc gagttcgact tgaacaggtg tcacccctct tgcgccagcg
      601 taggtgtctc tcatactgaa attgaaccgg ctgcgcgaac tgccacctat gttcaacagg
      661 acagcccctt gctcgggacc attcttgtac tcgagcttgc ttccccgccc ctgtcggaaa
      721 tatggggaga tcagcgtatc ggtacacaat cctccgcccc agatcccatc gtagaagttg
      781 aaggtcatcc cctgaatcat cgggttcgtt gttcctggcg cagcatcata ctctccctga
      841 atggtcatct gatctgcctt ccagccctta ttcagaagac tgagattcca gtcatccaca
      901 cgcaggttct gatcaacctg ggcgaatccg tactgtccaa acatgataca catgctgaac
      961 aatacaaaga cactaagttt agtcgtcttc attgttcaaa gattcctttc tctagcttct
     1021 tgaagaaatg ctctacagtc tgcctctcat gttttgcaga ctgctccgac aaacaaggca
     1081 tctcctgttg tggagacacc actcctctgt tccctccgca gacctcaccc cctttcgacg
     1141 catgtgcgtc ttctctcggt caccccagtt ggagactccg atctcctaga gtccctctat
     1201 cctgatgggc tgatcaaaac gctcctccag aatttggtcg aagatctttc gtatctcgtt
     1261 gtcttcaatt gacggataag cctgtttcag cttttggagt acctcatcgg tgccaacaag
     1321 ccctaatgtc gttcgggcat gttcccgaac aagcacagag gaatcggata gagcagcgac
     1381 tagtgcatca atactctccc ctgactgtgc atgttcgagt gctacagccg cctcagcacg
     1441 aacttcctcg ttgaaatcgg tttggagatt gtgagaaagc accgactcca agttctcccc
     1501 ttgttcaaag gctatgtccc ctagatcttt gattgcgtcc gtcctcgact cgggtgaccc
     1561 ttgggagagt tgctcaagag ctttcaccac atcacggtta tcaccgacat agagtgcgag
     1621 aggcgtaaaa tgaatctggt gtcggtatcc ttggtcaaga tcatcaagat taccagcaag
     1681 gggaactcgc gcagcccgtg ctactgcgtc caatatggcg gacggcgtcg gatcgtccac
     1741 ctgaacttct gctttcagat tggcattgcc cgatcgaaag agttccaagc caagttgatc
     1801 gcagagagag gcaagtgcct catccgcaga gatacgtccc gcgggcagtt caatgttcct
     1861 ggccttcttc ggttgagatc ctgccaaagg agatacagat gacgaagcgg gcttctctga
     1921 agattcagcg ccttgggagc gattcgcgtc ttgtaagtcc agattgtccc cattgggtct
     1981 gtctgtatcc tggctgcctc taggcgaggc atctttctcg gcatgggttt ctggctgctt
     2041 ctcaccgagt gttccctttg tgcttacaag accattcctt gaggacgaat caggaagcac
     2101 tcgagccaac cctacaagga ctacgacgcc aagaaccgct aacagaacta tctctgtgcg
     2161 agaaagcctc atcagcaaac ctccaaggca atacgtctct tctgcttttt ttattatctg
     2221 agagtcagaa ctctcccaca gtctctgcac aatcgaataa tctgttttgg atcatttgtc
     2281 aagaagaatg aatcgctacc gatcggatcg ttttagagta ctctacatat agtgcagttg
     2341 tgccaccgaa aaccaagcgg tagataaagt gagtttcttg tgtaaaagga acgaggccac
     2401 aggaaaggat gatcgactcg atcttgcagc agtctgcgat cgatagaaaa aaacacgcac
     2461 aaagacatcc ggcatccttc gatcaatacc ctgaagaacg gtaagatatg tgtgacacgc
     2521 tactcggg
//
LOCUS       DAOQUC010000515         2526 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_49970_length_2526_cov_3.545933, whole genome shotgun sequence.
ACCESSION   DAOQUC010000515 DAOQUC010000000
VERSION     DAOQUC010000515.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2526
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49970_length_2526_cov_3.545933"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            164..1249
                     /locus_tag="PKH07_09435"
     CDS             164..1249
                     /locus_tag="PKH07_09435"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HNT35206.1"
                     /translation="MSNEVQEYFERLAARIRSASDVDSLNRYLNLYLKESNHDRRYFH
                     FSVQASQQEGRVQLTGEVQYPEQIEGLVSLLKTLGFETENHVAVLTEGTRQGDRYGIV
                     KVPFAGFYVEPNPETEQVTQARYGERVQVISGPRDGHYLVRSHDAYLGWASCECFEYC
                     PKDRWLTWDNCPRVRFGTSYDHDGVTLLANTELPLRDKDQVEFPDGALQDLSQITGSG
                     AQVERLPFAATSAIREKIIELARTWLETKYVWGARTNQGTDCSGFAQSMYGFCGIYLP
                     RDARHQFLVGRVCGGRDFMQALPGDLLFFLGRTGVVTHVAISLGDYRFIHAGGGRVHE
                     ASLSPNDPDYQDQRAHTFFCAKRVIPA"
     gene            1246..2079
                     /locus_tag="PKH07_09440"
     CDS             1246..2079
                     /locus_tag="PKH07_09440"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HNT35207.1"
                     /translation="MIGSLRQCFSKPLSCQWFAADTDSERGLDDWGYSHYLSRWCAKQ
                     VGALGVVRKESNSVFRKVFFVLIALCLLGVSYSQAQGQPFKWGVVDIDRILDEYKFVA
                     QAFKKLEQDYTREQSVLDARADEINRIDKEMRLKDELTADESSDTKKGKEEKLKAETL
                     EYFRRGREMSEKLERKKSFHIRKILEVVGATIEDFGKKEGYQAIFKRNFLAYVEENSP
                     NDVTDKIIALLNAAPVPDLAKIEQEFEDFSKAEAAREATGSKTDTSGIEQSPTSGRTA
                     P"
     gene            2165..>2526
                     /locus_tag="PKH07_09445"
     CDS             2165..>2526
                     /locus_tag="PKH07_09445"
                     /inference="COORDINATES: protein motif:HMM:NF016494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LpxD N-terminal domain-containing protein"
                     /protein_id="HNT35208.1"
                     /translation="MPVLTTGELAQELGAILEGDATVPLSGVSGIVEADPGDLSFIHN
                     PKYIDKIAQTQASALIVPMTLETDFRPILRAENPYLTFAKAISILAPGRQLPPPGIHS
                     TAVIGQNVSIGEGASIQA"
BASE COUNT          602 a          627 c          717 g          580 t
ORIGIN      
        1 ggctgttccc gcttcggcag cgtcgcagag cctctgcaac cgaagcatac ctccaacccc
       61 gccgtaactg agccgacact tcggttggag gtaatccctt gacaacgtct ggcacaatga
      121 ctagcttgcc tgaaaatcag aaacgatgga gcgaggtaat atcttgagca acgaagtaca
      181 ggagtatttt gagaggctgg cggcgagaat ccggtcggcg agtgatgtgg atagcctgaa
      241 tcggtatctc aatctgtatt tgaaggaaag caatcacgac agaaggtatt tccatttcag
      301 cgttcaggcc tcacaacaag aggggcgtgt gcaactgacg ggtgaggttc agtatccaga
      361 gcagattgaa ggtcttgtga gtctgttgaa gaccttgggg tttgagacgg agaatcacgt
      421 tgcagtgctt acagaaggca ctcgacaggg ggacagatac ggcattgtga aagtcccctt
      481 tgctggcttc tatgtggaac ccaaccctga aacagagcag gtgacacagg cgcgatacgg
      541 agaacgggtg caggtgattt ctggtcctcg tgacgggcac tacctcgttc gaagccatga
      601 tgcctacttg ggttgggcgt cctgtgagtg ctttgaatac tgtcccaaag accgttggct
      661 gacctgggac aactgtccga gggttcgctt cggcacatcc tatgatcacg acggagtgac
      721 tctgcttgcc aacacagagt tgccccttcg cgacaaggat caagtggaat tccccgacgg
      781 ggccctgcag gatctttctc agattaccgg ttccggtgca caggtggaaa ggcttccttt
      841 cgccgcgaca tctgccatcc gcgagaaaat catcgaactg gcgcgaacct ggcttgagac
      901 caaatatgtc tggggtgcca gaaccaatca aggcacagac tgttccggtt ttgcgcagtc
      961 catgtatggt ttctgcggca tctaccttcc gagagatgca cgacaccagt tcttggttgg
     1021 ccgtgtgtgt ggagggaggg atttcatgca ggcgcttccc ggagacctgc tcttcttcct
     1081 gggacgtacc ggagtcgtga ctcatgtggc gatttctctg ggcgactatc gctttatcca
     1141 tgccggaggc ggtcgggtgc atgaggccag cctgagtcca aacgatccgg attaccagga
     1201 tcaacgcgcc cacacattct tctgcgccaa aagagtcatt ccggcatgat cggaagcctc
     1261 aggcagtgtt tcagcaaacc actcagttgc caatggtttg ctgctgatac cgactcagaa
     1321 cgcgggcttg acgactgggg ctattcccat tatctttccc gatggtgcgc caaacaggtt
     1381 ggcgctttag gtgttgtacg gaaggagtcc aatagcgtgt ttcgaaaagt tttctttgta
     1441 ctgatagcat tgtgtctttt gggagtgtct tattcacagg cacagggtca gccattcaaa
     1501 tggggggttg ttgatattga tcgaattctt gatgaataca aatttgtggc gcaggcattc
     1561 aagaagttgg agcaggacta cactcgtgag cagagcgttc tggatgcccg agcggatgag
     1621 atcaaccgaa tcgataagga aatgcgcctc aaagatgaac tgacggctga tgagtcctca
     1681 gacaccaaga agggcaaaga agaaaagctg aaagcagaga cgctggagta cttccgtcgt
     1741 ggccgtgaga tgagcgagaa acttgagcgc aagaaatcct ttcatattcg caagattctt
     1801 gaggtcgttg gcgcaacaat cgaagacttc ggcaaaaaag aaggctatca ggccatcttc
     1861 aagcgcaact tcctcgccta tgtagaggag aactcaccga acgatgtgac cgataagatc
     1921 atcgctcttc tgaacgcggc tccagtgcct gatcttgcga agattgaaca ggagtttgaa
     1981 gacttttcga aggctgaggc ggcaagggaa gcaaccggtt cgaagactga tacttcaggg
     2041 attgagcagt cgcccacgtc cggccgaact gcaccgtaaa gccgagggaa attcagactg
     2101 ttttgggggt agactcctct gaattcgggg gaggttcggg agtcactgac agggagatta
     2161 gaggatgccc gttttaacca caggtgagct ggcacaagag ctcggtgcca tcctggaagg
     2221 ggacgctacc gttcccctct caggcgtttc gggcattgtg gaggcagatc ccggagatct
     2281 ctctttcatc cacaacccga agtacattga caagatcgct caaacacagg cttcggcgct
     2341 cattgttccg atgactcttg agaccgattt ccgccccatc ttgcgagcgg aaaaccctta
     2401 tttgactttt gccaaggcca tttcaatcct cgctccggga cgccaactgc ctcccccggg
     2461 gattcactct acggcggtta tcggacagaa cgtttccatc ggtgagggcg cttcaattca
     2521 ggcgca
//
LOCUS       DAOQUC010000516         2520 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_50146_length_2520_cov_3.424746, whole genome shotgun sequence.
ACCESSION   DAOQUC010000516 DAOQUC010000000
VERSION     DAOQUC010000516.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2520)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2520)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2520
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50146_length_2520_cov_3.424746"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(181..1482)
                     /locus_tag="PKH07_09450"
     CDS             complement(181..1482)
                     /locus_tag="PKH07_09450"
                     /inference="COORDINATES: protein motif:HMM:NF016612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HNT35209.1"
                     /translation="MNAGFCSVKITPEIGTPIAGNYRADNLTHGVFHDIFSRSVCLRD
                     GDSLCSLTSVDLCAIAAWQVDAIRERVVRATPLAKENVTVAAIHNHSGPTTGVLTPGG
                     ERQDVIDRLIEQIADSIIGAYEDLQPVSLRLGRAEEAELPNNRRVWCQDGKIHMNWEK
                     HDTSFWIQEAGPIDPELLTLSFHDPHGSVRGIIVNYTLHPAVLAGDNNLTSADYVGFM
                     VRALSSELPGSPEVLFFNGAEGNVNHINPWDPDQIRGFKEAERIGRRVAMQAATAYAS
                     AEPMESPELVSIQKIIPVPRRKVSEEKIAWAQGVLAKWDGKPITLVDGCPDEVYAQAA
                     LKLASLQHEMVEAEVQIHQIGDLRIVTLPGEFFVEYGLRIKQVAGQGRTMVIGLANGV
                     IGYLPTPKAFEEGGYEITLGHQSYLDETAGEIVLAAIERML"
     gene            1793..2422
                     /locus_tag="PKH07_09455"
     CDS             1793..2422
                     /locus_tag="PKH07_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020249000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNT35210.1"
                     /translation="MKQHHGFTLIELLIVVAIIGILAAIAIPNFLAAQIRAKVARARA
                     ELQAINTCLESYRVDQNDYPPNDGVYNVLPIQITTPVSYISNSRLIDPFSAHLTHPVF
                     GDLARYYTYNRIVTSQELVELTSLGQWIPPIEAVDSPTFNRDAFRKYGKWRTLSNGPD
                     STYSDPATFGSDYPLFGADILYDPTNGVVSWGNILRTQKSPIGRIDPVP"
BASE COUNT          611 a          711 c          598 g          600 t
ORIGIN      
        1 gagaatcgaa gacaagatcc cggtgattct ctccagtggc tacaatgaac aggaagtcac
       61 ttcgcgattc aacgagcaag gactcgcagg ctttatcgaa aagccctatc gtctcggcag
      121 actcacacta gccctacgca gatgtctgga gcaaagcgca gatcgctccg agacttagaa
      181 tcaaagcatc cgctcaatgg cggcaagaac aatctctccg gcagtctcgt ccagataact
      241 ctgatgtcca agcgttatct cgtagccacc ctcttcaaag gccttcggcg ttggaagata
      301 tccaatgact ccattggcaa ggccaataac catggttcgg ccctgacctg ccacctgctt
      361 gatcctgagt ccgtattcaa caaaaaactc cccaggcaga gtgacgattc gcaaatctcc
      421 aatctgatga atctggactt ccgcttcaac catttcgtgc tgaagtgaag cgagttttag
      481 cgccgcctgg gcatacacct catcaggaca tccgtccaca agggtaattg gcttaccatc
      541 ccacttggca agaactccct gcgcccacgc aatcttctcc tcactgactt tgcgtcgagg
      601 aacaggaatg atcttctgaa tggagacaag ttctggcgac tccatcggct ctgccgaggc
      661 ataagccgtg gccgcctgca tcgcgactct gcgtccgatg cgttccgcct ctttgaatcc
      721 tcgaatctga tctggatccc acggattgat atggttcacg tttccttcgg cgccattgaa
      781 gaaaaggact tctggggagc cgggtagttc gctgctcaat gcgcgtacca tgaaaccaac
      841 atagtccgct gaggtaagat tgttgtcgcc agcaagaact gcgggatgca gggtgtaatt
      901 cacaatgatt cccctgacgc ttccatgcgg gtcatggaaa ctcaatgtca gcagttcagg
      961 atcgatcggg cctgcttctt ggatccagaa ggacgtgtca tgcttctccc agttcatgtg
     1021 aatcttcccg tcctgacacc agacacgacg gttgttgggc agttctgcct cctccgcacg
     1081 acccagacgc aatgagactg gctgcagatc ctcataggcg ccaatgattg aatccgcaat
     1141 ctgttcaatc agacgatcga tgacgtcctg tctctctccg cctggcgtca gcaccccggt
     1201 ggtggggcct gagtgattgt ggatggctgc aacggtaacg ttctccttcg caagcggcgt
     1261 cgcccgaact acacgctctc gaatggcgtc cacctgccag gcggcgatcg cgcagagatc
     1321 aacactcgtc agactacaca gcgagtctcc atcccgtaag catacagaac gcgagaatat
     1381 gtcgtgaaag acaccatggg taaggttgtc cgctctgtag ttgcccgcga ttggtgttcc
     1441 tatctctggc gtgatcttga ctgaacagaa tccggcattc atctttgcct cctggactaa
     1501 gatctttgtt atggtcagca ctgatgggaa tgtacacgat ccagagcgcc acgccaacaa
     1561 cggcgtgaaa gactacagaa tcgtgaaagc cttgttgcgg atgctctggt tttgagaaag
     1621 gactccgttg atttttcttg atccctggac cagcaaacgg tatatttgga ataatctttg
     1681 ttcaagagtc aaatgttgaa ctgagaatgg gttattttgc tttgggaatg aaggttactc
     1741 gaaaacatct gatttgattc aggcatgact cgtcagggag gatatctgca tcatgaaaca
     1801 gcaccatgga ttcacactta tcgaactttt gatcgtcgtc gccatcatcg gcatccttgc
     1861 agcgatagcg atcccgaatt tcctcgccgc acagattcgg gcgaaagttg cccgtgcccg
     1921 tgcggagctt caggccatca acacctgcct ggaaagctat cgcgtagacc agaacgatta
     1981 tccacccaac gatggagtct acaacgtctt gcccattcag ataaccactc cggtctccta
     2041 tatctcaaac tcgcggctga ttgacccatt ctccgcacac ttgactcacc cagtttttgg
     2101 agaccttgcg cgatactaca cctacaacag aatcgtgact tcccaggaac tcgtggaact
     2161 gacttctctc ggccagtgga ttcctcccat tgaagcagtg gattctccaa cattcaacag
     2221 agatgccttc aggaaatacg gtaaatggag aaccctgtcc aatggccctg actccaccta
     2281 ttccgaccca gccaccttcg gatctgatta cccgctcttt ggtgctgaca tcctctacga
     2341 tccaaccaac ggtgtcgtca gttggggaaa tatcctgaga acacaaaaaa gcccgatcgg
     2401 aaggatcgat cccgtccctt gatcacattc gagcctgcag tgcacaccga aagataagac
     2461 agatcgccct gcagaattca tagatcctgc agggcgtcat ctcgacttat tctcgtgtga
//
LOCUS       DAOQUC010000517         2519 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_50178_length_2519_cov_3.241477, whole genome shotgun sequence.
ACCESSION   DAOQUC010000517 DAOQUC010000000
VERSION     DAOQUC010000517.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2519
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50178_length_2519_cov_3.241477"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..914
                     /locus_tag="PKH07_09460"
     CDS             <1..914
                     /locus_tag="PKH07_09460"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNT35211.1"
                     /translation="FAQEHGGTPYSDYSRMFDEAELDAVSICTPGGNHLPLVVAAADH
                     GVHALVEKPLEVSKSALDEMIRACKTNNVKLAGIFQLRTTPVFKTVRKIVTSGKLGRM
                     ILADAYLKYYRTPEYYQSASWRGTYAMDGGGCLMNQGIHCVDQLLWCMGSVKSVFAHA
                     DHLVHKIESEDTAVCVLKYANGAFGVIEGATSAYPGLKYRLEFHGDCGTLRVDGHQIV
                     QFESSLVSPEEAQRIVASIVNVPNESQRDPLALGIDNHVEQVHDFINALIEDREPLVP
                     GYEARRSVDLILAIYESARTGKEINLI"
     gene            927..1793
                     /locus_tag="PKH07_09465"
     CDS             927..1793
                     /locus_tag="PKH07_09465"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNT35212.1"
                     /translation="MLRVGIVGSENSHTLHFAKLINVEKKFRGTKVVAVWGEEPNHTQ
                     NVAQQTGIPEIVTKPEKMVGKVDLAIVDHRHARYHIPAATPLVRAGIPVLVDKPFSWN
                     VPEGKRLLALAAKKKTLVTSYSCVRYSDGFLKDKETIQGLGKITSVDYFGPCDVNSPY
                     GGIFFYGIHQVEMMTAHLGIDVVAVQFNKGTTNDHTATVYYKDGPIVTLHLLKDYKGG
                     FRYTFCGAGGTVHLPLDYSGLYEKGLKAFFRMVKSGKNDFSEKELLAPVAILETLAKS
                     IQSGQREKIKKF"
     gene            1836..>2519
                     /locus_tag="PKH07_09470"
     CDS             1836..>2519
                     /locus_tag="PKH07_09470"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HNT35213.1"
                     /translation="MDPKRLVGIALICFGVVFYVQRNIVEAVQGHGNDFAHLYLGARA
                     LQQNVNPYDGAEIRRLAEQSGIPRMNPYVYPPLMAIAMKPLAGLRYDEARQIWFLVNQ
                     VFLLLSFVLLLLLWRPKSPMLAAGFLAVVYAFFFPLTRTLTAGQLNALLLLLLAVAAC
                     LERSGWSFPAGAVMGLASIIKVYPAIFLVYYVGRGNWKAVCSMVIAIVVLLGISVSVA
                     GVKTHLDYFP"
BASE COUNT          578 a          641 c          684 g          616 t
ORIGIN      
        1 agttcgctca agagcacggt ggaactcctt actcggacta cagtcggatg tttgatgaag
       61 ccgaactcga cgcggtatct atctgcacac cgggtggcaa tcatcttccc cttgtggtcg
      121 ctgcggcaga tcacggcgtc catgcgctcg tcgagaaacc gctcgaagtt tccaaatctg
      181 cgttggatga gatgatcaga gcctgcaaga caaacaacgt caaacttgcc ggcatttttc
      241 agttgcgcac aactccggtt tttaagaccg ttcgaaagat tgtcacctcg ggaaaacttg
      301 gaaggatgat tctggcagac gcctatctga agtactaccg aacacccgaa tactaccaaa
      361 gtgcttcctg gcgcggcacc tatgcgatgg acggtggcgg atgcctgatg aatcagggca
      421 ttcactgcgt ggatcagctg ctgtggtgta tgggttcggt caagagcgtc tttgcacacg
      481 cagatcacct ggtgcacaaa atcgaatcgg aagacaccgc ggtctgtgtg ctcaaatatg
      541 ccaacggtgc ctttggagtg atcgagggcg ctacatccgc ctatcctggc ctgaagtatc
      601 ggctggagtt tcatggcgac tgcggaacgt tgcgtgtgga cgggcatcag attgtccagt
      661 ttgagagttc gcttgtttcg cctgaagagg cccaacgcat cgtcgccagt atcgtcaatg
      721 ttcccaatga atcccagcgc gatcctctgg cactcgggat agacaatcat gtggagcagg
      781 ttcacgattt tatcaatgcc ctcatcgagg atcgtgagcc tttggtgccg ggatacgaag
      841 cacgcagatc ggttgatctg atcctggcga tctacgaatc agcccgaaca ggcaaggaaa
      901 tcaacctcat ctgaaggaga gagaagatgt tgagagtcgg tattgtcgga agtgagaatt
      961 cccacacact ccattttgca aaactcatca atgtggagaa gaagtttcga ggcaccaagg
     1021 tcgtggcagt atggggggag gaacccaacc acacacagaa tgtcgctcag caaacaggca
     1081 ttccagagat cgtcaccaag cccgagaaga tggtgggaaa agtggatctt gccatcgtgg
     1141 atcatcgcca tgccaggtat cacattcccg cagcaacccc tctcgtgagg gctggaattc
     1201 cggtacttgt ggacaaacca ttttcctgga acgttccgga aggcaagagg cttctggcac
     1261 tggccgccaa gaagaagacg cttgtcactt cgtacagttg cgtccgctac agcgatggat
     1321 tcctgaagga caaagagaca atccaaggac tgggcaagat cacctctgtg gattacttcg
     1381 gcccatgcga tgtcaactct ccctacggag gaatcttctt ctacggcatt caccaggtgg
     1441 agatgatgac cgcgcatctt ggaattgatg ttgttgcggt tcagttcaat aaaggcacga
     1501 ccaatgatca cacggcaacg gtctactaca aggacggtcc aatcgtcacc ctgcacttgc
     1561 tgaaggacta caagggcggg ttccgctaca ctttctgcgg tgcgggtgga acggttcacc
     1621 ttcctttgga ttactcagga ttgtacgaaa aaggcctcaa ggcattcttc cgaatggtca
     1681 agagcggcaa aaatgacttc tccgaaaagg agttgctcgc tccggtggca attcttgaaa
     1741 cgctggcaaa gtccatccaa tcaggacaga gagagaagat caagaagttc tagctgtggt
     1801 gttgtttcca cacaggaacg acggagtcgg agaacatgga tcccaagcgg ttggtcggca
     1861 tcgcgttgat ctgcttcggc gtggttttct acgtccagag aaacatcgtt gaagccgttc
     1921 agggtcatgg aaacgatttt gcacatctct acctcggagc ccgcgccctc caacagaatg
     1981 tgaatcctta cgacggtgct gagataaggc gtctggcaga acagtctggc attccgcgga
     2041 tgaatcccta tgtctatccc ccgttgatgg cgattgcaat gaagccgctt gccggtcttc
     2101 gctatgatga agccaggcaa atctggtttc ttgtgaatca ggtctttctc ctgttgtcct
     2161 tcgtgcttct tctcttgctg tggcgaccga agtctccgat gctggcagca ggttttcttg
     2221 ccgttgtgta tgccttcttc tttcctctga ccaggacact gaccgctggt cagctaaacg
     2281 cactgcttct gctcttgctg gctgtagcgg cgtgccttga gagaagtggc tggtctttcc
     2341 ccgcaggcgc tgtgatggga ttggcatcca tcattaaggt ctaccctgcc attttccttg
     2401 tctattatgt gggtcgtggc aattggaaag cggtctgctc catggtgatt gcgatcgtcg
     2461 ttctgttggg aatctctgtc agcgttgccg gtgtcaagac ccatcttgac tactttcct
//
LOCUS       DAOQUC010000518         2519 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_50185_length_2519_cov_2.877841, whole genome shotgun sequence.
ACCESSION   DAOQUC010000518 DAOQUC010000000
VERSION     DAOQUC010000518.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2519
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50185_length_2519_cov_2.877841"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..769
                     /locus_tag="PKH07_09475"
     CDS             <1..769
                     /locus_tag="PKH07_09475"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNT35214.1"
                     /translation="QGIITTWEDFERYQWPKPEDVNYRSFEAISSVLPDEMKVIGQYG
                     DIFTQVWMTMGFEAFSFAQIEQPDLIEALFEKFGELIVGLFETMAEFDCVGALWYSDD
                     IAYTEGLMVSPVFLRTYLFPFMSRIGAVCRERDIPFLYHSDGVLYEVLNDLEQCGVNA
                     LQPVEPKAMDIVELKKKVAGRFCLIGNVELDAFLCRGTPETVEQEVKRLIREVAPGGG
                     YCLGSSNTVPRFVKKENYRAMIEAGRKYGRYPINVPM"
     gene            766..2280
                     /locus_tag="PKH07_09480"
     CDS             766..2280
                     /locus_tag="PKH07_09480"
                     /inference="COORDINATES: protein motif:HMM:NF019311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA14 domain-containing protein"
                     /protein_id="HNT35215.1"
                     /translation="MNTPKVSSSLVEDEPKELRQAFGWLNPIFRTLSFLPLTLALFGM
                     LGTTALIRQQQFQVSYHESTSETETPLWVGTVRHIRFINDDGYFLPDSLSTSDFSLRF
                     RARLWISTAGEHTFALDSDDASWLFLDGRQILDNGGRHRMLGRTATVLLEQGWHDLEL
                     DYRQYDLESVLMLRWARPEQDELELLRSPYVYLPETEVLPLSSLSVQRWFSFGGVVLL
                     LVQLWSLAVIVFCFPKARAKMSARLALIALLGTLGGLGVLWSPSRPAPAGLWAMPAPT
                     DACSLEETGFFKGLFSRRVSEGSILSSGPLTHAIFPFFTRFWGSPSAAASAVFLCAVL
                     SFVGLQYRLGKDFFASKAAGIVASAFLAVVTAFMLREGITFAGESLVYGSLMLLFSWR
                     ICRQRSLWSEPMNPLLFILFTVLGFWTSWVVLCFFLSSLTMMALGRKWEASIVSLATF
                     TSKALLVLAGVAVLLVALSMAFPAWRESSQLPTAFLVFALAIGHIGAGLIVKTS"
BASE COUNT          489 a          609 c          706 g          715 t
ORIGIN      
        1 acaagggatc ataaccacat gggaagattt tgaacgctac cagtggccga agcccgaaga
       61 cgtcaactat cgttcattcg aagcgatcag cagtgtcctt cccgatgaga tgaaggtcat
      121 tggacagtac ggcgatattt ttacccaggt ctggatgacc atgggttttg aggccttttc
      181 ctttgcgcag attgagcagc cggatctgat cgaggctctt tttgagaaat tcggggagct
      241 tattgttggc ctctttgaaa ccatggcgga attcgattgt gtgggtgccc tttggtacag
      301 cgatgatatc gcctatacag aagggctgat ggtttccccg gtttttctcc gaacatatct
      361 atttccgttt atgagtcgca ttggtgcagt ctgtcgagaa agagacatcc cttttctcta
      421 tcactccgat ggtgtgctgt atgaagtgct caacgatctg gaacaatgtg gggtgaatgc
      481 tcttcagccc gttgagccta aggccatgga tatcgtggag ctgaagaaga aggttgccgg
      541 acggttttgt ctgattggca atgtcgaact ggatgctttt ctgtgtcggg gcactcctga
      601 gacagttgag caggaagtca agcgtctgat ccgtgaggtg gctccgggtg gcggttactg
      661 tctgggttcg agcaatacgg tgcctcgctt tgtcaaaaag gagaactacc gtgccatgat
      721 cgaagcgggc aggaagtacg gtcgctatcc cattaatgtc ccaatgtgaa cacaccaaaa
      781 gtctcatcca gtcttgttga ggatgaaccg aaggaactca ggcaggcctt cggctggctg
      841 aatcccattt tcaggacgct gtcgtttctc ccgcttacac tggccttgtt cggaatgctt
      901 ggcacgacgg ctctgatccg tcagcagcaa tttcaagtct cctaccatga gagcacttcg
      961 gagactgaga cccctctctg ggtgggtaca gtgcggcaca ttcgattcat caacgatgac
     1021 ggatattttc ttcccgattc cctgagcacg agtgatttca gtcttcgttt tcgggcaaga
     1081 ctttggatct ctacagcggg agaacatact tttgcactgg actctgatga tgcctcctgg
     1141 ctctttctgg acggcaggca gatacttgac aatggtggcc gacacaggat gctcggcagg
     1201 actgccactg tcctgttgga acaggggtgg catgacctgg aactggacta ccggcaatac
     1261 gacctggagt ctgttctcat gcttcgatgg gcacgtcccg aacaggatga attggaactt
     1321 ctgagatcgc cttacgtcta cttacctgag accgaagtgc tgccactttc cagcctgtct
     1381 gtgcagagat ggttttcctt cggcggtgtc gttctcttgc tggtgcaact gtggtctctc
     1441 gcagttattg tcttctgctt cccaaaggcc agagccaaga tgagtgcgag gctggctttg
     1501 attgctttgt tgggtacgct cggtggctta ggtgttttgt ggtctccgag caggccagcg
     1561 cctgcaggcc tgtgggctat gcccgctcct acagacgctt gctcattgga agaaactggt
     1621 tttttcaaag gcttgttttc caggagagtc tcagaaggtt ccattctgtc atcaggtccc
     1681 ttgacgcatg cgatctttcc tttcttcacg cgtttctggg gttcgccgtc tgctgcagca
     1741 agtgcggttt ttttgtgtgc ggtcttgtcc tttgtgggac ttcagtacag gcttggaaag
     1801 gatttctttg ccagcaaagc ggcgggaatt gttgccagtg cgtttctggc tgttgtcacg
     1861 gcctttatgc tgcgagaggg tatcactttt gctggggaat ctctcgtata tggttctctg
     1921 atgctgcttt tcagttggag aatatgtcgg cagagaagcc tgtggtcgga acccatgaat
     1981 cctttgttat tcattctttt cacggtgctg ggattctgga cgagttgggt tgtcctgtgc
     2041 ttttttctct cgtcgctgac tatgatggct ttgggtagaa aatgggaggc cagcatagtc
     2101 tcattggcga cgttcacttc gaaggctttg cttgtgctgg caggggtggc tgtcctgctg
     2161 gtcgcattat ccatggcttt cccggcgtgg cgagagtcca gtcagttgcc cacagccttc
     2221 ttggttttcg ctctggctat cgggcacatt ggagccggtc tgattgtcaa gacttcgtag
     2281 ggacaccact tcctgcgcac tttggctgtc agagcaccat ccttaacttg tagaaccctg
     2341 agagatccct catttcattc cgcaaagagg ggtaggttgg gtcgtgaccc aacatctgaa
     2401 ctccttctgc gacgggatag aatccggatc acgccaacgg agcgggggca tcttgccccc
     2461 gaatggaaga atgtggggcg agacgcccca cctcctatca gcgttttcta actctctct
//
LOCUS       DAOQUC010000519         2517 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_50228_length_2517_cov_4.119415, whole genome shotgun sequence.
ACCESSION   DAOQUC010000519 DAOQUC010000000
VERSION     DAOQUC010000519.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2517
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50228_length_2517_cov_4.119415"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(145..822)
                     /locus_tag="PKH07_09485"
     CDS             complement(145..822)
                     /locus_tag="PKH07_09485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35216.1"
                     /translation="MIEVSFIPSAFIMELIHPPLNHPDRILKEVFLEVSEPHRYSSFQ
                     LVGGRPGAVLASGDRRHCEILTDRLIIREERTESTFRDFAQDSLNLVDAVRRRVPIPI
                     FLTTAFTIRSLVPLPPNQTDSVRLLVEKCWKMPSESFSSFNRPLGGIGFRFVFPPVQE
                     DRSEVQVRLEPSFGDRRMLFVEIHYRFFNPVQTREELEKVAETVYDFQSRQVADFLNQ
                     MVREESL"
     gene            complement(830..1342)
                     /locus_tag="PKH07_09490"
     CDS             complement(830..1342)
                     /locus_tag="PKH07_09490"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF012304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HNT35217.1"
                     /translation="MERIPPKPKAEKIEDVDLNDLSGWKPLEVPQGEYVVYVDGCSKG
                     NPGEAAYAFVAFAHGGVEVLRGGDALGKKTNNQAEYEGLIASLEICLKMGLLNILVVS
                     DSELLVKQIQGLYRVKNPDLRTLWGQAKSLAHRFEKFDIRFTPRKGNAAADKLANEAL
                     RIGLKSRRGR"
     gene            complement(1433..2239)
                     /gene="larE"
                     /locus_tag="PKH07_09495"
                     /pseudo
     CDS             complement(1433..2239)
                     /gene="larE"
                     /locus_tag="PKH07_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007778222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
BASE COUNT          579 a          703 c          635 g          600 t
ORIGIN      
        1 agcggtaaat ctcgcgatag gctgcctcgc gccttgcctt caatacgtcc agaaacttgc
       61 tcatagcttt cctttggcct taggtgtcac cgcaccgcgg ctgttggatt tgacgagaaa
      121 gatatcgtga gaacccgatt gatttcaaag ggattcttct cgaaccatct gattgaggaa
      181 atccgccacc tgtctcgact gaaaatcata gaccgtctca gcgactttct ccagttcctc
      241 acgggtctga acgggattga agaaacggta atgaatctca acgaacagca tacgcctgtc
      301 accaaaagat ggctccagtc gcacctgcac ttcggatcgg tcttcctgca cgggtgggaa
      361 gacaaaccga aaaccgattc ctcccagagg tcggttaaac gaggagaaag actcggacgg
      421 catcttccag catttttcca cgagaagacg aacactgtcg gtctgatttg gtggcagtgg
      481 aaccaggctt ctgatcgtga aagccgttgt gagaaagatc ggaatgggaa cccgccgcct
      541 gacggcatct acaaggttga gcgaatcctg cgcaaaatcc cgaaatgtgg attcggttcg
      601 ctcctcccga atgatgaggc ggtcggtgag tatctcacaa tggcgacggt cacccgaagc
      661 caagaccgca cccggcctgc cacccaccag ctggaaggac gaataccgat gcggttccga
      721 gacttcgaga aagacttcct tgagaattcg gtcgggatga ttcagtggag gatggatcaa
      781 ctccatgatg aatgcggatg gaataaaact gacttcgatc ataatggtgt caccttccac
      841 gccttgattt cagtccgatt ctgagtgcct cgtttgccag tttgtccgct gcggcattgc
      901 cctttctggg ggtgaagcga atatcaaact tctcaaaccg atgcgccaga ctcttcgcct
      961 gcccccagag tgttcgaaga tcgggattct tcactcggta aagcccctga atctgcttga
     1021 ccagcagttc gctgtcggag actacgagaa tattcaagag acccatcttt agacagattt
     1081 caagcgaggc gatcagacct tcgtattccg cctgattgtt ggtcttcttc cccaaagcgt
     1141 ctcctccgcg taggacttcc accccgccgt gggcgaaggc aacaaaggcg taagctgctt
     1201 cgcccggatt gcccttggaa cagccgtcaa cgtacacaac gtattcgccc tgtggaacct
     1261 ccagaggctt ccagcccgaa aggtcgttca gatccacatc ctcgatcttt tcagcctttg
     1321 gtttgggtgg gatccgctcc atcggtggga atctctcgtc ctgaaaaagc gcctcctctg
     1381 aaagagggaa agtttcggaa caggcttgct gtccaccgtt gggtatggag agttcaggca
     1441 aagtggcgtt catgcttcca gttcgatatc cttccaaatc catggcaaca aattggtagc
     1501 ctgcagcctt gaagtgctcc acgatcttct cgcgattctt caccacccga tcaaacgact
     1561 cgatatccgt ttcgatccgc gcaatgtcct gatggcttcg cacccgatac tgtcgaacgc
     1621 ccagttgttt cagcagcaac tcggcgtcgg aaactctctt caacttctcc tccgtgatcc
     1681 tttctccata cggaaaccgc gaagcaagac aagccaccgc gggtttgtcc cagacggaaa
     1741 gattcagctg ccgtgccaac tgacgcacgt cgtccttggt catttccgct tccagaagag
     1801 gactgcgaac gcccagttcc tgtgctgcct tgtgaccggg tctgtagtct gacaaatccg
     1861 aaacagtggc tccgacaagg acagaggcca acccttcctt gtcggcgatt gtccgcagct
     1921 gtgtaaaaag ctctttctta caataatagc agcgctcagg tggattgtca cgaaagccag
     1981 gcaaatccag ctcacaggaa tgaatcgtcc tgtgccgggc accgatctcc tttgctactc
     2041 gacgcgcctc ttcggcttcc catgccgggt acgttggcga gagggcggtg actgcgagaa
     2101 ccctatcgcc cagaacgtcc tgtgccactt tgagcagcag gctgctgtcc agtccccccg
     2161 aaaatgcgac aaccgcggac tccatttctt tgatgagttc tttcaacttt tcatatttct
     2221 gcatctcgtc acactccagg gtcgggtcaa aacaccttgc gaccttggac actgtaccca
     2281 acacgctcat tgacatcaag tttcggacaa ctatagtata ccaaatatgt ccagcacggt
     2341 tgtgcaggca ttgtgtgcct gccagacgtg agtgcattct gaagattctg tttggggacg
     2401 atttgtgaag cgagtttttg tcgcagaaga cgatctgaca tcgctgaagc ttctgggcgc
     2461 ggtcttgaag aaatggggat atgacgtggt ctccgcttct gatggaagca ccgcttg
//
LOCUS       DAOQUC010000520         2517 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_50233_length_2517_cov_3.468725, whole genome shotgun sequence.
ACCESSION   DAOQUC010000520 DAOQUC010000000
VERSION     DAOQUC010000520.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2517
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50233_length_2517_cov_3.468725"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..827)
                     /locus_tag="PKH07_09500"
     CDS             complement(<1..827)
                     /locus_tag="PKH07_09500"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNT35218.1"
                     /translation="MTAVNRLYIPSRRRSRWQMQRQQGGFTLLEILAAVFIFVLVIGC
                     IWSTFHSTLAAFEAGEKTMDTYQDARVTMQNFTRDLRRAVSPNSRWSNDQKAILNTIA
                     QEEQRQMQEGIFDLANDPFANDPSLNRDIRFVGTSNEVTLTLSEQLKDPKFPYDLCQV
                     RYFADKEREQLVKSTVQSIVALRREEWRADRILNETDYREVHDMTASSFEVQKEQVIG
                     NSIKELTLRYFDGAEWRDEWDSDQPIEPEEDEYEEETEEEIEEHWESYNYIESEKVGL
                     "
     gene            complement(824..1285)
                     /locus_tag="PKH07_09505"
     CDS             complement(824..1285)
                     /locus_tag="PKH07_09505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35219.1"
                     /translation="MSQISPQRLFPKHRCSSTRRSSTGGFLLMEVLAAVIILTVVMVA
                     LTQSTVQSMNSASEMKDYTRVIMLAQQKMWELENEYAFTQKGSTSQDGIFAEPFSAYN
                     WEADIEADEERLVFVISLKVSWVFRQKDRWFNLTSEVPMRGDEVERKRDQR"
     gene            complement(1282..1863)
                     /locus_tag="PKH07_09510"
     CDS             complement(1282..1863)
                     /locus_tag="PKH07_09510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35220.1"
                     /translation="MNANADPKCGKPARFGAVETSGFTLMEMMAVIIIIMMMTVMAVP
                     AFRGFYRKTQEDTVAAQLARLLRYAHQKAVYRSQVTRVDIELEKGEFWVFVPAKESDR
                     FSQAKKELESVLPRFFAFESIWFPEVQKRQDRGRVSVHFFPDGSAEEARIVLLSEQAG
                     DFQPKRILVFVRPNTGKIVTRVLEAGEDVEDFL"
     gene            complement(1860..2294)
                     /gene="gspG"
                     /locus_tag="PKH07_09515"
     CDS             complement(1860..2294)
                     /gene="gspG"
                     /locus_tag="PKH07_09515"
                     /inference="COORDINATES: protein motif:HMM:TIGR01710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HNT35221.1"
                     /translation="MRDISKQKGFTLVELMLVVVIIGLLAGLAVPNIMKQLGKAKIGK
                     AQGDITTLDNALSMFAMQVGDYPTTEQGLKALMTNLDNNPEWNGPYLRKGIPSDPWKE
                     AYVYRRESNHENVDFDLYSKGPDKQENTDDDIGNWSTESKVE"
     gene            complement(2314..>2517)
                     /locus_tag="PKH07_09520"
     CDS             complement(2314..>2517)
                     /locus_tag="PKH07_09520"
                     /inference="COORDINATES: protein motif:HMM:NF019934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspG"
                     /protein_id="HNT35222.1"
                     /translation="QEGLRALMENLRNDQDWRGPYVEGRDMPKDPWGQYYVYRRTPDP
                     EVRFVLLSMGPDGREGTDDDVTI"
BASE COUNT          537 a          734 c          607 g          639 t
ORIGIN      
        1 aggccaacct tctcgctttc gatatagtta taggattccc agtgttcctc gatttcttcc
       61 tcagtctctt cttcgtattc gtcttcctca ggctctatgg gttgatcgga atcccactca
      121 tcacgccact ctgcaccatc aaagtaccga agtgtgagtt ccttgatcga attgccaata
      181 acctgttcct tctgcacctc gaaactgctt gccgtcatgt catggacttc gcgatagtct
      241 gtttcgttca agatacggtc tgccctccat tcctcccgtc ggagcgcgac aatgctctga
      301 accgtgctct tgaccaactg ctctcgttct ttgtcggcaa agtaccgaac ttggcacaga
      361 tcgtatggaa acttgggatc tttgagctgc tccgacagtg tcagagtaac ttcattgctg
      421 gtgccaacga aacgaatatc ccgattgagt gagggatcat tggcgaaagg atcgttggcg
      481 aggtcgaata tgccttcctg catttggcgc tgttcttcct gtgcgatcgt gttcagaatc
      541 gccttctgat cgttgctcca gcgggaattc ggtgaaacgg cgcgtcgtaa gtcacgtgtg
      601 aagttctgca tggtgacacg ggcatcctgg taggtgtcca tggtcttctc gcctgcctca
      661 aaggccgcca gcgtggaatg gaacgtactc catatgcacc cgatgaccaa cacaaagatg
      721 aataccgcag caagaatctc cagcagcgta aagccacctt gttgtcgctg catctgccaa
      781 cgcgaccggc gacgacttgg aatgtacaat cggttcactg cggtcatctt tgatctcgtt
      841 tgcgctccac ttcgtcgcct cgcatgggga cttcactcgt caggttgaac cagcgatcct
      901 tctgccgaaa tacccaggag accttcaggg aaatgacgaa gaccagacgt tcttcgtctg
      961 cttcgatgtc cgcttcccag ttgtatgcac tgaacggttc ggcgaaaatc ccgtcctgcg
     1021 aggtggagcc tttctgcgtg aacgcatatt cgttctccag ctcccacatt ttctgttgag
     1081 caagcatgat cacccgcgtg tagtccttca tctcgctggc agagttcatg gactgaacgg
     1141 tggactgcgt cagtgccacc atgacaacgg tcagaatgat gaccgcagcc aacacttcca
     1201 tcagcaaaaa gccaccggta gaggaccttc tggtactcga gcaccgatgc ttcgggaaga
     1261 gcctttgggg agagatctgg ctcacaggaa gtcctccacg tcttcgcctg cttccaagac
     1321 ccgagtgact attttgcccg tgttgggacg aacaaacaca aggattcgtt tgggttgaaa
     1381 gtcacccgcc tgttcggaca gaagaacgat ccgtgcctct tcggcagagc catcgggaaa
     1441 gaaatggaca ctgacgcgcc cacgatcctg cctcttttgg acttcgggaa accagatact
     1501 ttcaaacgcg aagaacctgg gcagaacaga ctcgagttcc ttcttggcct gactgaaacg
     1561 gtcgctttct ttcgctggaa cgaaaaccca gaactccccc ttctccagct cgatatccac
     1621 gcgggtcact tgtgaacgat agaccgcctt ctggtgggca tatcgcagaa gccttgccaa
     1681 ctgtgcagca actgtatctt cctgggtctt ccggtagaat ccccgaaacg caggaaccgc
     1741 catgaccgtc atcatcatga taatgatgat cacggccatc atttccatca aggtgaagcc
     1801 actcgtttcg accgcgccga atctggctgg cttcccgcat ttcggatcgg cgtttgcatt
     1861 cattcgactt tcgattccgt gctccagttg ccgatgtcat catcggtgtt ttcctgcttg
     1921 tcaggccctt tggagtagag gtcgaaatcc acattttcat ggttgctctc acgccggtac
     1981 acatacgcct ctttccacgg atcgctcgga atgcccttgc gcaggtaagg tccattccac
     2041 tcggggttgt tgtccaggtt cgtcatgagg gccttgagtc cctgttccgt tgtaggatag
     2101 tcgccaacct gcatggcgaa catggacaga gcgttatcca gcgtggtgat atccccttgg
     2161 gctttgccga tcttggcctt acccagctgc ttcatgatgt tgggaacagc cagtccggca
     2221 agcagtccaa tgatcacgac caccaacatc aactccacca acgtgaaacc cttctgcttc
     2281 gagatatctc tcatcaaaaa ccctccttga atgctatatg gttacgtcgt catccgtgcc
     2341 ttcacgtccg tcaggcccca tcgaaagaag cacaaaccgt acctcgggat ccggcgtccg
     2401 ccgatagaca tagtactgtc cccatggatc cttgggcatg tcccgtcctt ccacataggg
     2461 accgcgccag tcctgatcgt ttctcaagtt ctccatcaat gcccgaagtc cttcctg
//
LOCUS       DAOQUC010000521         2517 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_50241_length_2517_cov_2.635256, whole genome shotgun sequence.
ACCESSION   DAOQUC010000521 DAOQUC010000000
VERSION     DAOQUC010000521.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2517
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50241_length_2517_cov_2.635256"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(15..>2517)
                     /locus_tag="PKH07_09525"
     CDS             complement(15..>2517)
                     /locus_tag="PKH07_09525"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNT35223.1"
                     /translation="VGQIVGGDIQSWSGSGPHANGSLGVNITANGAWGPGSVPINEYR
                     NGWGAWETWGGTSRSCPLAAGIAALVYEAYQQKHGVLPTPQTVKDILMNSAVDQKQDV
                     FRQGSGRINAGRAVQLASENSGVEVTPSQWIPGDYRGEAYDFYARLVYGGDIRTITHT
                     IQNHSDQPVTLNVSTVGLEEIGYRDFEIQTVASPLEEMNSKKPDYIRLLESDVEKNIP
                     ANTDLIVLEAIFPFEDFDTDFNGNANSVTPPRENAYQLLVYNWTDINQNGKLFDDSLG
                     SVPNLVEANEIESGEFTRMDYGYNNGTQLKCAIADPLRRMGDGIWFGIRHRAGSKGYS
                     TTLQVRVRFYAQVACPWLSASVDQVVVPANGQANPQAQVEIPMNMAGGFYSAKLLLQD
                     ASGQTETTVIPVQICVAADLDSNVVTFGGGGTDTTPYSNNVVRGDFSWQGRDEAGDGR
                     FFCVDAVHPSPGDQIIVRTEWQDSLPTDIDTIVAGPASDSFSNPLSSFYDPSFGPGGL
                     EWVAGASSPGRPTCTFYTATGSTVDYACGALKDGLHGIFLHNVLFSGKNFAVPFSTQV
                     STFNTAPSEFHLPVGDVTATVSLMSATDMTDFRVHAYGPSLLEKFRDQPVERWNRDDP
                     MSKAWVYDFTVQNGGLLDFRLSSGEPDLDLYLFKDGWDGSAPDGQFDFYNEKVIASET
                     SLSSEFIWWRIPPNGFYRIAVVGYALSSNSGLFDLDATVVQGTLTSVLPTSFAELKDR
                     VTASLALGLSLPNAGQYGVFMLNGPAEAQYLIPTLLTINAGAWGDLDGDGEITLRDLV
                     QASIYWGRSAPANVDADDDGIMTAIDLLEFIRSLK"
BASE COUNT          583 a          728 c          635 g          571 t
ORIGIN      
        1 ctgttccgat cagatcactt cagacttctg atgaactcca gcaggtcaat cgccgtcatg
       61 atgccatcat cgtctgcatc aacattggcc ggagcagatc tgccccagta gatggaggct
      121 tgaaccaaat ccctgagggt tatttcaccg tcgccatcaa gatcgcccca agcccctgca
      181 ttgatcgtca acagtgtcgg gatcagatat tgcgcctcgg ccggcccgtt gagcataaag
      241 acgccgtatt gtcccgcatt gggaaggctc aaccccagag caagactcgc tgtcactcta
      301 tccttcaact cggcgaatga tgtgggcaac acacttgtga gcgttccttg aaccactgtg
      361 gcatcgaggt cgaacagccc tgagttcgag gagagagcat atccgactac cgctattctg
      421 tagaaaccgt tgggaggaat tctccaccag ataaactcgg atgaaagact ggtttccgaa
      481 gcgatcactt tctcgttgta gaagtcaaac tgaccatcgg gagcggagcc atcccatcca
      541 tccttgaaga gatacaagtc cagatcgggc tccccgctgc tcagtcgaaa gtcgagaagc
      601 ccaccgtttt gtacggtgaa gtcatagacc catgctttgc tcatcggatc atcccgattc
      661 catctttcga cgggttgatc ccgaaacttc tccaggagcg acggcccgta ggcatggacg
      721 cgaaagtccg tcatatccgt tgcactcatc agactgacag ttgccgtgac gtctcccacc
      781 ggcagatgaa actctgatgg tgctgtgttg aacgtgctga cctgggttga aaaaggaaca
      841 gcaaaattct tcccgctgaa gagcacgttg tgcaggaaga ttccgtggag accgtccttg
      901 agcgcaccac aggcgtagtc aactgtgctc cctgtcgcag tgtaaaaggt gcaggtgggc
      961 cgccccggac tgcttgcacc agcaacccac tccaggcccc ccggcccgaa actgggatca
     1021 tagaaactgc tcagcggatt ggagaaggaa tcactcgccg gaccggcgac aatagtgtca
     1081 atgtcggtcg gcaggctgtc ctgccattca gtcctcacga tgatctgatc ccctggagat
     1141 ggatggactg catccacaca gaagaaacga ccgtcccctg cttcatcgcg accttgccag
     1201 gaaaaatcac cgcgaacgac gttgttgctg taaggggtcg tgtcagtccc acccccgcca
     1261 aaggtcacca catttgaatc caaatcggcc gcgacacaga tctgaacagg gatgacagtg
     1321 gtctcagtct gtccggaagc gtcctgcagc agcagtttgg cggagtaaaa accaccggcc
     1381 atattcatcg gaatctcaac ctgcgcctga ggattggcct gcccgtttgc agggacgacc
     1441 acctgatcca ccgacgcgct taaccaggga catgcgacct gtgcgtagaa cctaaccctt
     1501 acctgaagcg tcgttgagta acctttggag ccagctctgt ggcgaatccc aaaccatatc
     1561 ccgtcgccca tccgccgaag gggatctgcg atcgcgcatt tcaactgcgt tccgttgttg
     1621 tagccgtaat ccatacgagt gaactcaccg gattctatct cgttggcttc cacaagattc
     1681 ggaacacttc ccagagaatc atcgaacagt ttcccgttct ggttgatgtc cgtccagttg
     1741 tatacaagca gttggtacgc gttttctctg ggtggtgtga ctgaattggc gtttccgttg
     1801 aaatcggtgt caaaatcctc gaatgggaaa atggcttcca agacaatcag atccgtattc
     1861 gcagggatgt tcttctccac gtcgctctca agaagccgaa tataatctgg ctttttcgaa
     1921 ttcatctctt ccaagggaga agcgactgtc tggatttcaa aatccctgta gccgatttcc
     1981 tccaggccga ccgtgctgac gttcagggtc acgggctgat ccgagtgatt ctgaatcgtg
     2041 tgcgtgatgg ttctgatatc cccaccatag acgagcctgg catagaaatc gtaagcctct
     2101 ccccggtaat cgccaggaat ccattgggac ggagtgacct ctacacccga gttctcggaa
     2161 gccaactgaa cggcacgacc tgcgttgatt cgtcccgatc cttgacggaa tacatcctgc
     2221 ttttgatcaa cggcggagtt catcaggata tctttgaccg tctgcggcgt gggcagaaca
     2281 ccgtgcttct gttgatacgc ttcatataca agggcagcga tccctgcagc gagtggacaa
     2341 gagcgactcg ttcctcccca ggtttcccat gcgccccagc cgtttctgta ttcgttgatc
     2401 ggcacactgc cgggtcccca ggcaccattg gcggttatgt tcactccgag agaaccattt
     2461 gcatgaggac ccgaaccact ccaactctga atatcaccgc caacgatctg acccacc
//
LOCUS       DAOQUC010000522         2513 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_50343_length_2513_cov_4.146867, whole genome shotgun sequence.
ACCESSION   DAOQUC010000522 DAOQUC010000000
VERSION     DAOQUC010000522.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2513
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50343_length_2513_cov_4.146867"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..529
                     /locus_tag="PKH07_09530"
     CDS             <1..529
                     /locus_tag="PKH07_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012924111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35224.1"
                     /translation="VDTPTDTHVPTFTHTETPTLVPTETPTYTPTNIPTDTPTSVPSN
                     TPTDAPSDTPTRLPTDTPTELPTETPTDLPTDTPTDAPTDTPSETPTDIFTETPTDTP
                     EIQPTDTPTKRPTPKIRPDFNGDNVIDSEDALLLLNAYGTDDMRFDLNEDGVIDAEDM
                     FIFSSFWNQEQDSGN"
     gene            677..1969
                     /locus_tag="PKH07_09535"
     CDS             677..1969
                     /locus_tag="PKH07_09535"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNT35225.1"
                     /translation="MRQSQKGLLFIGIACTVLLFGCGQRVETTLRFWNGFTGPDGRTM
                     LRMVKRFNRENPDVHVLMQRMDWGTYYNKLFVAGIGNRSPEVFIIHTDVLERFIQAGF
                     LRSVDDLVGEPGSLDSGDFDANVWEAVVSGAKHYAVPLDVHPLGVYYNRALFRQAGIV
                     DASGEPKPPTNREEFLDTLKHLTRDTDGDGRTDQWGFVFTWQRTNVFTFMNQWGGRFF
                     NEKYDECLLNTPENVEALQFCVDLIQKHKFAPSPENFDSWIGFRQGKVGMVLEGIYML
                     ADVQKQQDLDYAGAPVPQVGAVKAAWASSHNMCLRADLSGDKLQAAWRLVRFLSDNSL
                     DWAEGGQVPVRKSLRDSARFETMSVPREFAKQIQDIRYMPKLPFVFEFYTEFDAAIEK
                     ALRGTLPPKQALDTATKNINEIIERHKSLQTQLRGQEP"
     gene            1966..>2513
                     /locus_tag="PKH07_09540"
     CDS             1966..>2513
                     /locus_tag="PKH07_09540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNT35226.1"
                     /translation="MRKNAWHRWSGYLFLLPYLVLFVSFLIVPLFYGLGLSLFQWEVL
                     SRVPPQLVGLGNFLEALRSSYFWKAFLATFRFVIMTVPLSVGFALFVACGINAIPFKR
                     QSFYRASIFLPTIISISVAGILWRWFFNGEFGLFNALLGHFGYKVPWLSDVNWAMKSI
                     VFMTLWWTVGGPMVVLLAGLQQ"
BASE COUNT          572 a          661 c          659 g          621 t
ORIGIN      
        1 ggttgatacg ccaacggata cacacgttcc aacattcact catactgaga cccccaccct
       61 cgtgccgaca gagactccaa cctatacacc cacgaacatc ccgacagata ccccgactag
      121 tgttccgagt aacacaccca cagatgctcc gagcgatact ccgacgcggc tcccaaccga
      181 caccccaacc gaactaccga cagaaacgcc aacagacttg ccgaccgaca ccccgacaga
      241 tgctcccaca gacacgcctt ctgaaacacc tacggacatc tttacggaga ctccaacgga
      301 tacgccggaa atccagccaa ctgatacccc aacaaagaga cctactccaa agattcgtcc
      361 tgatttcaac ggggacaatg tgattgactc ggaagacgca cttcttcttc tgaatgctta
      421 cggaaccgac gacatgcggt ttgacctgaa tgaagatggg gtcattgacg ccgaggatat
      481 gtttatcttt tcctcattct ggaaccagga acaagattcg ggaaactgag agcagaacgc
      541 ccggttcagt tagaagttca tacggcgcat cacggttatc cgtggtgcgc tgttggttct
      601 atctgtcgtt tgactcttct cgacgaagcg tctaatctcc tcctgcaatc gagctgttgt
      661 tgtctgagga gatgaagtga gacagtcaca gaaaggtctc ttgttcatag gaatcgcatg
      721 cactgtgctc ttgttcggtt gtggccaaag ggttgaaacc accttgcgtt tctggaatgg
      781 atttacgggt cccgacggcc gaacaatgct tcgaatggtc aagcgcttca atcgagaaaa
      841 ccccgatgtt catgtactca tgcagcgcat ggattggggg acttattaca acaaactctt
      901 cgtagccggc atcggcaacc gttctccgga agttttcatc atccatacgg acgttctgga
      961 gcgctttatc caggcgggat ttctgcgttc cgtggacgat ctggttggag agccagggag
     1021 tctggattca ggagatttcg acgccaatgt atgggaagcc gtcgtgagtg gagcaaagca
     1081 ctatgcggtg cctctggatg tccatccgct gggtgtctat tacaacagag cgctctttcg
     1141 tcaggcaggc atcgttgacg caagcgggga acccaagcca ccgacaaacc gagaggagtt
     1201 tctcgatacc ctcaagcacc ttactcgtga tacggatggc gatggcagaa ccgaccagtg
     1261 gggatttgtc ttcacctggc agcgaacaaa tgtcttcact tttatgaacc agtggggggg
     1321 aagattcttc aatgaaaagt acgacgagtg tctgctgaac acgcctgaga atgttgaagc
     1381 actgcagttc tgtgttgatc ttatccagaa acacaagttt gctccgtcgc ctgagaactt
     1441 cgactcttgg attggatttc ggcaagggaa ggtcgggatg gttctggaag gcatctacat
     1501 gctggcggat gtccagaaac agcaggatct ggattacgcc ggagcccctg ttccacaggt
     1561 tggtgccgtg aaggcggcat gggcgagttc tcacaatatg tgtcttcgtg ctgacctctc
     1621 cggcgacaaa ctgcaggctg catggcgtct cgtccgattt ctctccgaca acagtctcga
     1681 ctgggcggaa gggggacagg tgcctgttcg caagagcctt cgtgactccg ctcgattcga
     1741 aacgatgagc gttcctcggg agtttgccaa acagattcag gacatccgat atatgccgaa
     1801 actgccgttc gtctttgagt tctataccga atttgatgca gccatcgaaa aggcattgcg
     1861 tggaacgctg cccccgaagc aggcgcttga caccgcaaca aagaatatca acgaaattat
     1921 cgaacgccac aagtcgcttc aaacacagtt gagggggcag gaaccatgag gaagaacgcc
     1981 tggcatcgct ggagtggtta cctttttctc ttgccctatc tagtcctgtt tgtgtcgttt
     2041 ctgatcgttc ctctcttcta cggcttgggc ttgagtcttt tccagtggga agttctctct
     2101 cgggttcctc cacaactcgt cggtctgggc aactttcttg aggcgcttcg aagcagctac
     2161 ttctggaaag cgtttctcgc cacatttcga tttgtgataa tgactgtgcc tttgtccgtg
     2221 ggctttgctc ttttcgttgc ctgtgggatc aatgccattc cgttcaaacg acagtctttc
     2281 taccgggcgt ccatctttct tccgacgatc atctccatca gtgtggcggg aatcctctgg
     2341 cgctggttct tcaacggtga gtttggcttg ttcaatgcgc ttctgggaca cttcggatac
     2401 aaggtgccct ggctgtctga tgtgaactgg gcgatgaaat ccattgtgtt catgacgctc
     2461 tggtggacgg tggggggacc gatggtggtg ctcttggcgg gacttcagca gat
//
LOCUS       DAOQUC010000523         2513 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_50351_length_2513_cov_3.504068, whole genome shotgun sequence.
ACCESSION   DAOQUC010000523 DAOQUC010000000
VERSION     DAOQUC010000523.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2513
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50351_length_2513_cov_3.504068"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            501..2078
                     /locus_tag="PKH07_09545"
     CDS             501..2078
                     /locus_tag="PKH07_09545"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNT35227.1"
                     /translation="MRDWFQLSDNDLTKKVVLLLVAIVALRVSTFGAYPLLDPTEGRY
                     AEIGREMLQTGNWVTPQLHPGLPFLGKPPLSFWATAVFLKFFGINEISVRLASLVFIL
                     IMGLFSFLLFKEISGTRVALWGLLILSTTGLVNFIAGGVMTDPSLGATVTAAMVSFAM
                     ALRSKSRLKRILWGYGFFISLGLTSLAKGLVGWFLIGIALVLWTAWEKRWKEILRSLP
                     WITGILVMFAIAVPWHLLAEKENPGFLNYYIIGEHFMRFIQPEWEGDRYATPHDMPRG
                     TIVLFAVAAALPWTIVLGACAAHFWKTRQRIKVLLSDPWRSFLVFWALSPLILFTLSS
                     NIMITYVLPGMPAFAMLTAMALQSLMVNSTAHQRPWYLKTQTLLASSAVVPVGFMIIA
                     LTILPAQGIGRSQKNIIARFRELTLDTHANLVYTSKIPFSADFYAHDFPVDFSEYSRH
                     EPEKVLMELRDTDLDFFVVKRSHLDDLPPEALRLTQQVGEFGKYTLRRELKPEEMKQV
                     LSASPEIRAVSPTFSVD"
     gene            complement(2112..>2513)
                     /locus_tag="PKH07_09550"
     CDS             complement(2112..>2513)
                     /locus_tag="PKH07_09550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35228.1"
                     /translation="EDGHGSKLLEWLQRRANHQLCVLAYDDREITYEGKKVVGPTGGT
                     FRATQRMVDSFITQIPISEERIGDLVHYSGMNGQIDMTVHLNPKNEILHTVLVGDMNG
                     FIYSMAVGTRYEKSACQFNGDLAYEKWIQPF"
BASE COUNT          547 a          660 c          650 g          656 t
ORIGIN      
        1 ggccatgatc agcctttcac ttgccgcagg tctgcctgct cggccaagcg cagaggcggg
       61 aatcaaccag ggcagagcca agccaactgc accaaccgat tgtctgataa atgttcttcg
      121 agtgatggag caggacattg gcggtttgac ctcctgagcc actcagacgt tggagaagat
      181 gacttaccgt cctgcggcca ctgtcccgca gaaacgactc aagtgagaca attcatagag
      241 ctttttcagt cttcaagcaa gcacggagga acgcaagcag gatgatatga ggcagggcct
      301 tatgttctga ataatcttgc tctccatgcg ccattgtcct cacgttgtgg cggctccatc
      361 cgtttgtaag aaaggcttgg cagtcaacac acagagaact gaaaacgtct gatagggctt
      421 tctgtcatgt tcttaagaga atcttcagtt gacccagtgc cgaagatact gaagaatccg
      481 cgccagagga gttcggagag atgagagatt ggtttcaact gtcggacaac gacctgacaa
      541 agaaggttgt tctcttgctt gttgccatcg tagcgttgag agtttcgact ttcggtgcct
      601 atccgctgct tgatccgacg gaagggcgct atgccgagat cggtcgcgaa atgctccaaa
      661 cgggtaactg ggtcacacct cagttgcatc ctggtctccc atttcttggc aagcctcccc
      721 tgtccttttg ggcaacagct gtctttctga agttcttcgg gatcaacgaa atctccgtac
      781 ggttggcttc gcttgttttc attctgatca tgggcttgtt ctcatttctt ctgtttaagg
      841 agatttcggg cacaagagta gccctttggg gactactgat cctcagcacc acgggtctgg
      901 tgaacttcat tgccggaggc gtgatgacag atccttcact gggtgcaaca gtcaccgcag
      961 caatggtctc attcgccatg gcactacgct caaaatcgag attgaagagg attctctggg
     1021 gctacggctt ctttatctca cttggtctga cttccttggc gaagggactc gttggctggt
     1081 ttcttatcgg cattgccctg gtgctctgga ctgcctggga gaagcgctgg aaagagattc
     1141 tccgcagctt accttggatc actggaatcc tcgtgatgtt tgccatcgct gttccctggc
     1201 acctgcttgc agaaaaggag aatcctggct ttctgaacta ctacatcatc ggggaacact
     1261 tcatgcgatt catccaaccg gaatgggaag gagatcgcta tgcaacgccg catgatatgc
     1321 cacgaggaac aatagtgctc tttgctgtgg cggcggcctt accctggacg attgtcctcg
     1381 gagcctgtgc ggcgcatttc tggaagaccc ggcagcgcat caaggtgttg ttgagcgacc
     1441 cgtggcgatc tttccttgtg ttctgggcgc tgtctccgtt gattctcttc acactctcaa
     1501 gcaacatcat gatcacctat gtgctccccg gaatgcctgc gtttgcgatg ctgaccgcca
     1561 tggcgttgca gagcctcatg gtgaactcga cggcgcacca gcggccatgg tatctgaaaa
     1621 cgcagactct gctggcatcg tccgctgtgg ttcctgtcgg attcatgatc attgctctga
     1681 caattcttcc agcacagggt atcggtcgtt cacagaagaa tatcattgct cgctttcgag
     1741 agctgacatt agatacgcac gccaacctgg tctacacatc caaaatcccc ttctccgccg
     1801 acttctatgc tcatgacttt cccgtagatt tttctgagta ctccaggcat gaacctgaga
     1861 aggttctcat ggaactgcgt gatacggact tggatttctt tgttgttaag agaagtcatc
     1921 tggatgattt gccgcctgaa gcactcaggc tgactcagca ggtgggcgag tttgggaaat
     1981 acacgctccg ccgggagctg aaacctgaag agatgaagca agtgctctcc gcaagccctg
     2041 agatcagagc ggtctcgccc accttctctg ttgactagac tttggagaaa acgactctat
     2101 gcgccgctcg cctaaaaagg ttggatccat ttctcatagg cgagatcgcc gttgaactgg
     2161 caggcgctct tctcgtatct ggttccgact gccatcgagt aaataaaccc gttcatgtct
     2221 ccgacaagaa ccgtgtgcag gatttcattc ttcggattca gatgtaccgt catgtctatc
     2281 tgaccattca ttcctgagta gtgaaccaga tcgccaattc gttcttcaga aatagggatt
     2341 tgtgtgatga aggagtccac catacgctgc gtggcgcgaa aggtgccacc ggtaggtcca
     2401 accactttct tgccttcata ggtgatctct cggtcatcgt aggcaagaac gcacagttgg
     2461 tggttggctc tccgctggag ccattccaac aacttgctgc catgcccgtc ttc
//
LOCUS       DAOQUC010000524         2512 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_50395_length_2512_cov_2.564510, whole genome shotgun sequence.
ACCESSION   DAOQUC010000524 DAOQUC010000000
VERSION     DAOQUC010000524.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2512
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50395_length_2512_cov_2.564510"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="PKH07_09555"
     CDS             <1..181
                     /locus_tag="PKH07_09555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35229.1"
                     /translation="ERSEEIKLKLKQANGMRRRIRNVMKGQAFPEDIREIAEGQVSGR
                     EQRAKMILESLGKSK"
     gene            278..1840
                     /gene="gguA"
                     /locus_tag="PKH07_09560"
     CDS             278..1840
                     /gene="gguA"
                     /locus_tag="PKH07_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HNT35230.1"
                     /translation="MSSSPLLEMRLIRKAFPGVLALDDVNLVAHAGEIHALVGENGAG
                     KSTLMKVLSGAYPDHGGEILIQGEKISIPNPRAAEQAGIATIYQELNLVPDLSVAENI
                     FLGREPVTRWRTLDRRRMERDAKALLGQLNCVVNPWRRLCELRLGEQQLVEVAKALSL
                     DARILIMDEPTSALSDQEAHRLFQVIRAMKAEGMTVLYISHKMDEVFALADRITVLRD
                     GRVVGTKRREDTVPEQIITMMVGRQIEEMFPVQSRQCKETVLRVEDLSTAHPTRVGER
                     FLDRVSFQLRQGEVVGIAGLMGSGRSELLEALFGSPRTPLLGGQILVDEQVRAIRKPM
                     DAIKSGLALVTEDRKGTGLFLQMTVGQNITIASLTKLARLGWIPGQAEIRVGKTYVEK
                     LSIRTPGLHTSVENLSGGNQQKTIIARWLLTEPRVLLLDDPTRGIDVGAKAELYRLMD
                     ALAGQGIGILMASSELPELLALCDRILVLCEGRLTGQLTRDEATEERIMALATIRHSA
                     HVGLSSVETAGN"
     gene            1895..>2512
                     /locus_tag="PKH07_09565"
     CDS             1895..>2512
                     /locus_tag="PKH07_09565"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNT35231.1"
                     /translation="MEQESRLHGLRQALRFLVRFQSLAGLIVVIVLAVVFSPVRRGAI
                     VFLNPDNLTDILRQISEKGILAVGMTLVILSAGIDLSVGSILAYSATLTAMMLRRWGW
                     PIAPAILVVLLSSLLLGTVNGVVTAKTRIQPFIVTLAMMSAARGLAKLLSNNSNVEIG
                     FGKEDSVARFMETVSSKFFVIPAFLIVVIVVYLLLRKTRFGRYTIA"
BASE COUNT          545 a          595 c          769 g          603 t
ORIGIN      
        1 ggagcgttcg gaagagatca aactgaaact caagcaggca aacggaatgc gtcgccgaat
       61 tcgcaatgtg atgaaggggc aggcctttcc tgaggatatc cgggagattg ctgagggaca
      121 ggtctccggg cgagagcagc gggcgaagat gatcctcgaa tcgctcggta agtccaagta
      181 atgatgtctg actgccaaac gagagtcttc cttcgcgcat ggagcgactg cgtcagttct
      241 catttcagtg cgttgtgaaa gggacacatc tggttacatg tcttcatcgc cacttttgga
      301 aatgcgtctg attcggaaag cctttcccgg cgtacttgcc ttggatgatg tgaatctcgt
      361 cgcacatgcg ggcgaaattc acgctctggt gggtgagaat ggagcgggca agtccactct
      421 gatgaaagtc ctcagtggcg cctatccaga tcacggcggt gagatactca tccagggtga
      481 aaagatatcc atcccgaatc cccgagccgc ggagcaggct ggcattgcga ctatctatca
      541 ggaactgaat ctcgttcctg atctctccgt ggcggagaac atcttccttg gtcgggagcc
      601 ggtgactcga tggcggactc ttgaccgacg ccgtatggag cgagacgcca aagcgctttt
      661 ggggcaactg aactgtgtcg tgaatccatg gcgacggttg tgtgaacttc gcctgggaga
      721 acagcaactg gtcgaagtgg ccaaggcgct ctcgctggac gcgagaatcc tcatcatgga
      781 cgagccaacc tccgcattga gcgaccagga ggcgcatcgg ttgttccaag tcatccgggc
      841 gatgaaggcc gaaggcatga ccgttcttta tatctcccac aaaatggatg aagtatttgc
      901 gctggccgat cggatcactg ttctcaggga cgggagagtc gtcgggacga agcggcgcga
      961 agacacggtt ccggaacaga tcatcactat gatggttggc agacagatag aagagatgtt
     1021 tcccgttcag agtcgtcagt gcaaagagac tgttctgcgc gttgaggatc tgagcaccgc
     1081 gcatccgact cgagttgggg aacggtttct cgatcgggtg agttttcagc tcagacaggg
     1141 agaggttgtc ggaattgccg gtctcatggg ttcggggaga agtgagttgc ttgaggcttt
     1201 gtttggttct cccagaacgc ccttgcttgg aggacagatc ctggtggatg aacaggtgcg
     1261 agcgattcga aagcctatgg atgcgatcaa atctggcttg gcacttgtta ctgaagatcg
     1321 gaaaggaact ggtctgtttc tccaaatgac ggtaggtcag aacattacca ttgccagcct
     1381 gacaaaactg gcgcggcttg gatggattcc aggacaggcc gagatccgtg tggggaagac
     1441 gtatgttgag aaactgtcca ttcggactcc tggcctgcac acgtcggttg agaacctctc
     1501 tggagggaat cagcagaaga cgattattgc gcgatggctg ctcacggaac ctcgtgtact
     1561 tcttctcgac gatccgaccc gtggaattga cgttggagca aaggcggaac tgtatcggtt
     1621 gatggacgct ttggcagggc aggggatcgg gattctgatg gcctcgtccg agttacctga
     1681 acttctggcg ctgtgcgatc ggattctcgt tctgtgcgag gggcgtctga cgggtcaatt
     1741 gacgcgcgat gaggcaaccg aggaacggat tatggcattg gcgaccatac gacattctgc
     1801 acatgttgga ttgtcttcgg ttgaaacggc agggaactga cgggacagtg ttgtcatcac
     1861 agcgctggaa ctgtctcttg gaggtctctg ggaggtggaa caagaaagca gacttcacgg
     1921 actcaggcag gctttgagat ttctcgtgag atttcagagt ctggcgggtc tgattgttgt
     1981 catcgtcctt gctgtggtgt tttcaccggt tcgtcgggga gcgattgtgt ttctcaaccc
     2041 ggacaacctc accgacattc tcagacagat cagcgagaag ggaattcttg cagtggggat
     2101 gaccctggtc attctctcgg caggcattga tctcagcgtc ggttccattc ttgcttattc
     2161 agcgacgttg acagcgatga tgcttcggcg atggggttgg cctattgcac cggcgattct
     2221 ggtggtgctc ctgagctctc tgctgctcgg cactgtcaac ggagttgtta cggcaaagac
     2281 cagaattcag ccgtttattg tgactctggc aatgatgagt gccgcgcgag ggttggccaa
     2341 actgctctcg aacaactcca acgtcgaaat cggttttggc aaagaagatt cagtggcgcg
     2401 atttatggag acggtgagca gcaagttctt cgtgattccc gccttcctga ttgtcgtcat
     2461 cgttgtgtat ctcttgcttc gcaagacacg tttcgggcga tacaccatcg cc
//
LOCUS       DAOQUC010000525         2509 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_50478_length_2509_cov_3.019152, whole genome shotgun sequence.
ACCESSION   DAOQUC010000525 DAOQUC010000000
VERSION     DAOQUC010000525.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2509
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50478_length_2509_cov_3.019152"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            91..339
                     /locus_tag="PKH07_09570"
     CDS             91..339
                     /locus_tag="PKH07_09570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35232.1"
                     /translation="MRQDKPRRKEPVFRTIAGAFDTGSAAWCRGTIICGRRVNLLSLL
                     AVVILCSAKDNFLDKGEKGYIEDPSSGKKKIIKDNIEQ"
     gene            341..>916
                     /locus_tag="PKH07_09575"
     CDS             341..>916
                     /locus_tag="PKH07_09575"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35233.1"
                     /translation="MNERQRFRETLLFGKPDKIPFAPGGPRESTLKVWHQQGLPQGAS
                     YMATLLETLGIQIPPTKPRTDVGVSFRMIPEFEEKVLEHRDGHYVVQDWMGAITEISD
                     QYDYTYIRHARDFVTRKWHKFPVESRADWENMKWRYDPKTPARFPGNFLERCNALQDR
                     DYVATIHFNAPFWQLREWCGFENLCLLMVDDP"
     assembly_gap    917..926
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <927..1468
                     /locus_tag="PKH07_09580"
     CDS             <927..1468
                     /locus_tag="PKH07_09580"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNT35234.1"
                     /translation="EMVEFWTDFVSQTMAPILDNVELDQVGMSEDMAFKCHSMISPKM
                     VREFLMPAYNCWVPQIKKSGCPIVFMDSDGYVADLIPLWIEAGINVNSPVEVAAENDI
                     VAYRQQYGKSMAYTGGIDKRAIAKGGKVMEQELERVVPPLLEDGGFIPGCDHGVPPDI
                     SWPSFVEYSRLLAKMTGWL"
     gene            1610..>2509
                     /locus_tag="PKH07_09585"
     CDS             1610..>2509
                     /locus_tag="PKH07_09585"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNT35235.1"
                     /translation="MSRSFRLIVLASASFGLATGCYDFILPFYMEAKNISFGNMGIIF
                     SLSGLAMVLGRVFVGRHSDRVGRKPYFLASLGLLSISTMSVPLLARAAALIISRTMTE
                     ISKELRATVHSVIVFDHVGERFLRFIGRASGPVYIMEGIGVIVAGLLLRWTGFTWTFV
                     LMGAQLGAITLIFRKGFQEGAGLVKGERDTESLLSWHLPKELWLIALAGFLQVAGMST
                     SHCFVMPLFFKDRFGLSPENVALILAVHRITLGLPMLTSGITYGMSQRSVWVVFSTAQ
                     SVTMLATGLAPNVLWAVLFWWAHD"
BASE COUNT          550 a          605 c          719 g          625 t
ORIGIN      
        1 ttttggattg cgtatgtggg cgttttgtgg ctaatcttgc gtcacttcta gatggctgga
       61 gtccgcaccg gcaaggagag atgcatcagg atgcgacagg ataaaccgag aagaaaagaa
      121 ccggtcttca gaacgattgc aggtgccttt gacacaggat ctgcagcatg gtgccgaggc
      181 acaatcattt gtggtagaag agtcaacctt ctatcgctct tggccgtcgt cattctctgc
      241 tcggcaaagg acaatttcct ggacaaaggt gagaaaggct atatcgaaga cccgagttcg
      301 ggaaagaaga agatcatcaa agacaacatc gagcaatgaa atgaacgaac gacagcggtt
      361 tcgagagacg cttctttttg gaaaaccaga caagatccct tttgcaccag gggggccgag
      421 agagtcaacg ctcaaagtgt ggcaccagca ggggcttccg caaggcgcca gttatatggc
      481 cactcttttg gaaactctcg gaatccagat tccaccgacg aagccacgaa cggatgtcgg
      541 tgtctccttt cggatgattc cggagttcga ggagaaggtg ctcgaacatc gggacggtca
      601 ttatgtggtt caggactgga tgggtgccat caccgagatc tccgatcagt atgactacac
      661 ctacatacga cacgccagag attttgtcac tcgcaaatgg cacaagtttc ctgtggaaag
      721 ccgcgcagac tgggagaaca tgaagtggcg ttatgacccg aagacgcctg cccgcttccc
      781 agggaatttt ctggagcgct gcaacgcact tcaagatcgg gactatgtgg caaccatcca
      841 tttcaacgcg ccgttctggc agcttcgtga atggtgtggc tttgagaatc tctgtctgct
      901 gatggtggat gatccgnnnn nnnnnnatga gatggtcgaa ttctggacgg atttcgtctc
      961 acagacaatg gcgccaattc tggacaatgt cgaactggat caggtcggga tgtctgagga
     1021 catggcattc aaatgccaca gtatgatttc gccgaagatg gtgcgcgagt ttctgatgcc
     1081 cgcctacaac tgttgggttc ctcagatcaa gaagagcggt tgccccatcg tgttcatgga
     1141 ctcggacgga tatgttgccg acctgattcc cctctggatc gaagcaggga tcaatgtgaa
     1201 tagtcctgtc gaagttgcgg cggaaaatga catcgtcgct taccgtcaac aatacggcaa
     1261 gagcatggcc tatacgggcg gcatagacaa acgtgccatc gccaagggcg gcaaggtcat
     1321 ggagcaggaa cttgagcgag ttgtgccacc actgctcgaa gacggtggct tcatcccagg
     1381 atgcgaccat ggcgttccgc cggacatttc gtggccgagt tttgtggaat actcgcgact
     1441 gctcgccaaa atgaccggat ggttgtaagc aggcgggcac ccaatctctt gacggcgtgg
     1501 cttgacatga ctcagtcagg caaggtaggc atagacggtt gccttgagtg agatggcatg
     1561 tcttccgatg gagaatttga tttgggagcg ctgtgagcgt caatagttta tgtcacgtag
     1621 ttttcgtttg attgttctgg caagtgcctc attcggtctg gcaacgggct gctacgattt
     1681 cattctgcct ttctacatgg aagccaagaa catttccttc ggaaatatgg gcatcatctt
     1741 ctccctctct ggactggcga tggtgctcgg acgagtcttt gtcggtcgtc attcggatcg
     1801 agtgggacga aagccctatt tcctggcgtc cctggggctg ctctcgatct cgaccatgtc
     1861 ggttccgctg cttgcccgtg cagctgcgtt gatcatctcc cgaacgatga cagagatcag
     1921 caaggaactg cgcgccacgg ttcattcggt gatcgttttt gatcacgtcg gtgagcgatt
     1981 tcttcgcttt attgggcgag ccagtggccc cgtttacatt atggaaggaa ttggggtcat
     2041 tgtggcggga ctgttgctcc gctggacagg gtttacgtgg actttcgtcc ttatgggagc
     2101 gcagttggga gccatcaccc tgatcttcag gaagggtttt caggaaggtg ccgggcttgt
     2161 caaaggggaa cgagataccg aatctcttct ttcctggcat cttcccaagg aactctggct
     2221 cattgcgttg gcggggtttc tccaagtagc gggaatgtcc accagccatt gttttgtgat
     2281 gcctttgttt ttcaaagacc gtttcggact ctctccggag aatgttgcac tgattctcgc
     2341 cgttcatcgg atcacgcttg gactccccat gctgacatcg gggatcacct atggaatgtc
     2401 acaacgcagt gtctgggtcg tcttctcgac ggctcagtcg gtgaccatgc tggcaacagg
     2461 tcttgctccg aatgtcctgt gggcagtgtt gttctggtgg gcacacgat
//
LOCUS       DAOQUC010000526         2509 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_50481_length_2509_cov_2.968215, whole genome shotgun sequence.
ACCESSION   DAOQUC010000526 DAOQUC010000000
VERSION     DAOQUC010000526.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2509
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50481_length_2509_cov_2.968215"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2147
                     /locus_tag="PKH07_09590"
     CDS             <1..2147
                     /locus_tag="PKH07_09590"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNT35236.1"
                     /translation="YAATKKSNELQASTYARLYDIPMTGLRFFTVYGPKGRPDMSIRM
                     FMEGLDRGEPIPMFGDGSFERDFTYVDDIIDGVVKALKVARGKKGWDHVFNLGEFRTT
                     TVRRMILFIVESLGRWKCDTSAEQLSEQQVQEAIDSLVNSGLVVRLPVPLGDVPKTCA
                     DISKAREILGYDPKTPLAEGIRKTARWHLEQGSSRQDPLRQAFHKAIREYWKIRNRAS
                     LDSLGRPKEPVYTESDLGVLLHCLDQFEKAAQISNSRCEKMLATRLIAWSWEVVGWIA
                     SSLSRDGEPKSAGLGRLTVHRKRMRLVQLLRDSGHDPLHDERLDTAMSLSREIINLTG
                     ERERAIVVAAAGYGSRVAKDLGGLNKKHSLFLGDEMLLLSLRNVIPFARRIIVVANER
                     NVSEIQERLERSEINESNGFQIEYVIQENRFGDGEAHLTAWKALRDFNGVALFVFADA
                     PTKTPETIERMLILKEALGPLVPLVVPCEDDDNPYSPIMLASDGLDRGRVLWNWQKAD
                     EQDFPEAALARKTYGLRNVGLFAAETCVFPFMEEFRDEVFSEYPRFRKWQQAMKQWQQ
                     KGSPESGKPKEPEYGFADLMKVLPRRGIEVAAPNLATRADRLNVNSLEDAETVKRVFL
                     ERNPLARVEVERRPDSSEIYVRMIDLDPKGNPIIYQGVPSLRHCTRFRFARGTRLDEA
                     KVEKQIGQHIEHISKRIYEELGIKVVCDTHNA"
     gene            2180..>2509
                     /locus_tag="PKH07_09595"
     CDS             2180..>2509
                     /locus_tag="PKH07_09595"
                     /inference="COORDINATES: protein motif:HMM:NF021387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HNT35237.1"
                     /translation="MKSDSCNVAFKFGQKSLETFIPKPFDLLSPRYTPALDCVERVVQ
                     ESLANPIGCGPLASLVRGKRSVCVVISDKTRPIPYQMILPPLLTVLLKTGLREEQISF
                     VIATGIHA"
BASE COUNT          596 a          600 c          709 g          604 t
ORIGIN      
        1 cttatgctgc aacgaagaaa tccaacgagt tgcaggcatc aacctacgcc aggctctatg
       61 acattccgat gacggggcta cgcttcttca cggtctacgg gccaaaaggc agaccggata
      121 tgtcgatcag aatgttcatg gaaggtctcg atcgcggtga gcccatcccg atgtttgggg
      181 atggttcttt tgagcgggat ttcacctatg ttgatgacat cattgacggc gttgtgaaag
      241 cgctgaaggt ggcacgtggg aagaagggtt gggatcatgt cttcaatctg ggtgagtttc
      301 gaacaaccac agttcggcgc atgatcttgt tcatcgtgga gtcgctcggt cggtggaagt
      361 gcgacaccag tgcagagcaa ctgtccgaac agcaggtgca ggaggcaatc gacagccttg
      421 tgaattccgg attggttgtg cgtcttcccg ttcccttggg ggatgttcca aagacctgtg
      481 ctgatatttc caaggcaagg gaaatccttg gatatgatcc aaagacacca cttgccgaag
      541 gaatccgcaa gactgcgcgg tggcatctgg agcagggttc ttctcgccaa gacccactgc
      601 gacaggcatt ccacaaagcc attcgggagt attggaagat ccgcaatcgt gcctctttgg
      661 acagcctggg acggcccaaa gagccagtct atactgaaag cgacctcggt gtcctactcc
      721 actgtttgga tcaattcgag aaagcagcgc agatatcgaa ctctcgatgt gaaaagatgc
      781 tggcaactcg cctgatcgcc tggtcatggg aagttgtggg ttggattgca tcgagtctct
      841 caagggatgg tgagccgaag tctgcaggtc ttggcagact gaccgttcat cgcaagagga
      901 tgaggctcgt tcagttgctt cgggactcag gccatgatcc cttgcacgat gagcgactgg
      961 acactgctat gagcctttct cgggagatta tcaacctgac tggggagagg gagcgagcca
     1021 ttgttgttgc cgccgcgggc tatggaagcc gtgtcgccaa agatcttggt ggtcttaaca
     1081 agaagcacag ccttttcctg ggcgatgaga tgttgctgct cagtctcaga aatgtcattc
     1141 cttttgcccg ccggattatc gtagttgcca acgagcgcaa cgtttcagaa atccaggaac
     1201 gcctcgagcg aagtgagatt aacgagagca acggatttca gattgaatat gtgattcagg
     1261 aaaaccgttt cggtgatgga gaagcacacc ttacggcgtg gaaggcactg cgggatttca
     1321 acggtgtggc gctctttgta ttcgctgacg caccgacaaa aactcctgag accatcgaga
     1381 ggatgctgat actgaaagag gcgcttggtc cattggttcc tcttgttgtc ccctgtgaag
     1441 atgacgacaa tccctattct ccgatcatgc ttgcctctga cgggctggat cgcggtcgcg
     1501 ttctctggaa ctggcagaaa gcggacgaac aggatttccc cgaggcggca ctggcaagga
     1561 agacctacgg gctccgaaac gtggggcttt ttgctgccga gacatgcgtt tttcctttca
     1621 tggaggaatt tcgagatgaa gtattctcgg agtatcctcg ctttaggaaa tggcaacagg
     1681 cgatgaaaca atggcagcaa aagggtagtc cggagtcagg caaaccaaag gaacctgagt
     1741 acggtttcgc ggacttgatg aaggtgctgc ccagacgtgg aattgaggtt gcggctccca
     1801 acttggccac ccgagcagac aggctgaacg tcaattcgct ggaagatgcg gagacggtta
     1861 aacgggtgtt tctcgaacgc aatcctctcg ctcgggtgga agttgaaagg cgcccagaca
     1921 gttccgaaat ctatgtcagg atgattgacc ttgacccgaa aggcaatccg atcatttatc
     1981 agggtgttcc gtcgcttcgt cattgcaccc gattccgatt cgccagaggt actcgtcttg
     2041 atgaggcgaa ggtcgagaag cagattgggc agcacattga gcatatctcc aagcggatct
     2101 atgaagaact cggaatcaaa gttgtttgtg atacgcacaa cgcttagaga cagtacttcg
     2161 gttttctgga atctcaacag tgaaatctga ctcctgcaac gttgcattca agttcggtca
     2221 gaagtcgttg gaaacgttca tcccaaagcc ttttgatctg ctgtctcctc gatacacgcc
     2281 agcattagac tgcgttgaga gagtcgttca agagtcactt gccaatccga tcggttgtgg
     2341 tccacttgca tcgcttgtga ggggcaaacg ctctgtttgt gttgtcattt ctgacaagac
     2401 gagaccgata ccctatcaga tgattcttcc gcccctgctg acagttctcc tgaaaaccgg
     2461 tttgcgggaa gaacagatca gttttgtcat agcgactggc attcatgca
//
LOCUS       DAOQUC010000527         2505 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_50600_length_2505_cov_2.722857, whole genome shotgun sequence.
ACCESSION   DAOQUC010000527 DAOQUC010000000
VERSION     DAOQUC010000527.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2505
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50600_length_2505_cov_2.722857"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..422
                     /locus_tag="PKH07_09600"
     CDS             <1..422
                     /locus_tag="PKH07_09600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35238.1"
                     /translation="AGPEQYDQSWAGTPQEKRKGTHACGTPCCIGGHAAIELRRREYL
                     AKGFATEEELDDPECSNLLMRDRDRFARCGIVYYNDSESIVTYLSRLCDIPYDISHHI
                     CWNNTLPNTTLEAAIRMLEYCRDTGIASWPRAMNGHI"
     gene            657..1259
                     /locus_tag="PKH07_09605"
     CDS             657..1259
                     /locus_tag="PKH07_09605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35239.1"
                     /translation="MSPDDADVSGIHDKGIYQKRMSRIKEYCTETYGVSMQENPDTGN
                     QESAISFCLRALCNIPQNVAEDLCFNLFEILSDMKKTKPGSTQREAVIAILQHCRDTG
                     QIEIPPGWAPPLTENQIELQELAREALSVQDACNLCGVAQGFARAMSRLRKLYPDTSV
                     YNSHPIAHAWINKMMDLSGTDLDGACFANVQEIIDNPPAI"
     gene            1269..1589
                     /locus_tag="PKH07_09610"
     CDS             1269..1589
                     /locus_tag="PKH07_09610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35240.1"
                     /translation="MRRKVNSGGLTLSVVKPTPTDTEEPLKDGDTAVLLQDMPADVDG
                     IVLKVGDCVLVEQVVVPGEIGRSLGAVTYIMISKKTYSPDGISEYNVRIPVLPEQLKK
                     VKLY"
     gene            1653..1859
                     /locus_tag="PKH07_09615"
     CDS             1653..1859
                     /locus_tag="PKH07_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35241.1"
                     /translation="MAKTAGVKVEVDKKKGTLTIVLPLQEPTASGSGKNMVVASTKGN
                     MRVDAEYDGKPLTIGVNAYYSANG"
     gene            1927..2235
                     /locus_tag="PKH07_09620"
     CDS             1927..2235
                     /locus_tag="PKH07_09620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35242.1"
                     /translation="MSFETWTIHERMEKIVGKVDLMDIRLSNHNKQSVQFVVRLTTSK
                     WGHPVIEIISRAKSGKKRVRRLQGVPMDSALLDQTIEYEKLKKKEKREKKLKKKEGVK
                     "
     gene            2232..>2505
                     /locus_tag="PKH07_09625"
     CDS             2232..>2505
                     /locus_tag="PKH07_09625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35243.1"
                     /translation="MTSQVYPGLQIIIDHLRTIPETDFDMNEGGEDSLRLPYGTKKEI
                     TCKSPCCIGGHARILLYNKIPEKERDLQQGRPLHIEQALAKLCDVPS"
BASE COUNT          727 a          593 c          639 g          546 t
ORIGIN      
        1 aggcgggacc agaacaatac gatcaaagct gggctggcac cccacaagaa aaaagaaaag
       61 gaactcatgc ttgtggaact ccctgctgca tcggcggaca cgcggcaatt gaactgagaa
      121 gacgcgaata ccttgccaaa ggatttgcga cagaggagga gctggacgac cctgagtgtt
      181 cgaatttgct gatgcgggat agggacaggt tcgccagatg cggcattgta tactataacg
      241 actccgaatc catcgtaacg tatctgtcaa gactgtgcga cattccctat gacatctccc
      301 atcatatttg ttggaacaac acccttccga atacgacgct ggaagcagcg atcagaatgc
      361 tggaatactg ccgagacact ggaatcgctt cttggccccg ggctatgaat ggtcatattt
      421 gacgttcttt tctgctccac tcttttcgaa aggtcgttac aatggcgtca ccagaatttc
      481 cagtccagtc aaatcccgtg tatccgggac ttcaaaacat catcgacatc ttggaatacc
      541 ttgttgatga tgagaacttc agttacataa atccgggtta tccccacctg tcgcacgatt
      601 gtagaacaca ctgttgcatc ggtgggcatg ctcagatcgc actggccatc cgatcaatgt
      661 ctcccgatga tgcagacgtc agtggaatac acgacaaagg tatttaccag aaaagaatgt
      721 cgcgtattaa agaatactgc acggagacct acggggtgtc catgcaggaa aaccctgata
      781 cagggaatca ggagtctgca atctcattct gtctgcgggc tctgtgtaac atcccacaaa
      841 atgtggcgga agatctctgt ttcaacctgt tcgaaatttt aagtgacatg aagaaaacaa
      901 agccgggaag cacacagcgt gaagccgtga ttgctattct ccaacactgt cgcgacacag
      961 gacagattga aatccccccg gggtgggcac ctccgttgac agaaaatcaa atagaactgc
     1021 aagagctggc ccgcgaggcg ttgtctgtac aagacgcctg taatctgtgt ggagtcgcac
     1081 agggtttcgc ccgagctatg tcgagattgc gaaaactgta tcctgacacc agcgtttaca
     1141 attcacaccc gatcgctcac gcgtggatca acaagatgat ggatctgtcc ggaacggatc
     1201 tggacggagc ctgttttgcg aatgttcaag agattattga caacccccca gcaatctaag
     1261 gaaaagtcat gcgaaggaaa gtaaactcag gtggtctcac tctgtccgta gtcaagccca
     1321 ccccaacaga cacagaagag ccgttgaaag atggagacac agccgtattg ctgcaggata
     1381 tgcctgcaga tgtagacggc attgttctga aagtggggga ctgcgtgctg gtggaacaag
     1441 tggtagtgcc tggggagatt ggacgttcgc ttggtgccgt tacatacatc atgatttcca
     1501 aaaagacgta cagcccggac ggcataagcg agtacaatgt tcgtattccg gttctgccgg
     1561 aacagttaaa gaaagtgaag ttgtactagg ctgcgaccta cagtctggta tgcgaattct
     1621 tctaggtctg gtttcttgaa aggtgtttag tgatggcaaa gaccgctggt gttaaggtgg
     1681 aagttgacaa gaaaaaggga actctgacaa ttgtgctgcc gctgcaggaa ccaacggcat
     1741 ccgggagtgg aaaaaacatg gtcgttgcct caaccaaagg caacatgcgt gttgacgcgg
     1801 agtacgacgg taagcccctg accatcggag tgaatgcgta ctactctgcc aacgggtagt
     1861 ccgtatcgtc caaagactaa gacagacgac aatggtgtcg tctgtctttt tagaaaggca
     1921 tttacaatgt catttgaaac atggaccatc cacgagcgga tggaaaaaat cgtcgggaaa
     1981 gtcgacctca tggatatacg actttccaat cacaacaaac aatccgtaca gtttgttgtc
     2041 cggctcacta catccaagtg gggacacccc gtcatcgaaa tcatttcccg ggccaaaagc
     2101 ggaaagaagc gagttcggcg gcttcaagga gtccccatgg attcagcttt gctcgatcaa
     2161 acgatcgagt acgaaaagtt gaagaagaag gaaaaacgag aaaagaaact gaaaaagaaa
     2221 gaaggtgtaa agtgacatca caagtatacc ccggtctgca gatcatcatc gaccatctgc
     2281 gtaccattcc agaaacggac ttcgatatga acgaaggagg agaggacagc cttcgccttc
     2341 catatggcac caaaaaggag attacctgca agagcccctg ctgtattgga ggtcacgccc
     2401 ggattctgct gtacaacaaa atccccgaaa aagaaaggga tctgcagcag ggcagaccac
     2461 ttcacattga acaggcacta gcaaaactat gcgacgttcc tagtg
//
LOCUS       DAOQUC010000528         2503 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_50662_length_2503_cov_2.825980, whole genome shotgun sequence.
ACCESSION   DAOQUC010000528 DAOQUC010000000
VERSION     DAOQUC010000528.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2503)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2503)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2503
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50662_length_2503_cov_2.825980"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..539
                     /locus_tag="PKH07_09630"
     CDS             <1..539
                     /locus_tag="PKH07_09630"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNT35244.1"
                     /translation="KVRIGEYSKGMMQRVGLAQSLMSDPQLLLLDEPLSGLDPIGRKE
                     ITDVILELGKQGKTIFFSSHILSDVETLCDRIGILNRGRLRSIGKVSELVGAKVTSIT
                     VEVADLPQERLAEIREKTTRVQDLGANQVLLEVPDQETVSHIVQVASASKAHILSITP
                     RKESLEDYFLREIKEESR"
     gene            539..1309
                     /locus_tag="PKH07_09635"
     CDS             539..1309
                     /locus_tag="PKH07_09635"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNT35245.1"
                     /translation="MRIWAIALNTFREATRNRVFYMILIFIVLMLLSSAALGMLSLGE
                     QDRVVKHLGLSAMNIFGLMLAMFVGVALVHEELENKTIYTLMSAGVSRGQFLFGKFLG
                     LTMTIVVNITMMTLLLTLLIFMTPGSHLTVSVYQAALMSLFEMMIIIAIAILFSVVTS
                     PVLAMILTFLMWVIGMMSQSLQLFALRLGELGHSVAKYVILGILLFLPRLDVFNLKDE
                     IVYSEVLDFRYLYMFYGLLYTGAVLAVSNFIFSRKDFQ"
     gene            1313..2368
                     /locus_tag="PKH07_09640"
     CDS             1313..2368
                     /locus_tag="PKH07_09640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35246.1"
                     /translation="MAWARKKVVRSVLVCLLLLATLGTQIGVQQLRSTQRVRDYIYLV
                     PDLKALKVISLSFRSLVADMLWLRAIQYFGGWYSQMKKQPMGFIHLINAVVYLDPRFV
                     QAYSFGAFCIAEGLDDYDAAVELLVRGAETNSENPQAWRLMFDAGFIRFYNQKRNEEA
                     KELIDRASQMPGADEFTKRTAAYIDSVSGRRDIARERYRAMYETAETELIRDLALKHL
                     IRIERDEHTEILKSAVEEFKRKMERKPRDLKELVEKGFLKAIPSDPGGSQYVYVAKSD
                     VVLLKEVLDIARDTLAAAIKTQAIAFEEARGRKPLSLQELIDERFVGADLTDPAGMHI
                     ELDLESYSIIFSETPES"
BASE COUNT          595 a          630 c          651 g          627 t
ORIGIN      
        1 caaaggttcg aatcggcgag tactccaaag gcatgatgca gagggttggg cttgcgcaat
       61 cgctgatgag tgacccacaa ctgcttctgc tcgatgagcc actctcgggt cttgacccca
      121 ttggaaggaa ggagattacc gacgtaatcc ttgagctggg aaagcaaggc aagaccatct
      181 tcttcagttc acatattctc agtgacgttg agaccctctg cgatcgaatc ggcatcctca
      241 accgaggacg cctcagaagc atcggcaaag tctcagaact ggtaggtgca aaggtcactt
      301 cgataacagt cgaagtggca gacctaccgc aagaacgcct cgcggaaata cgagagaaga
      361 caactcgagt tcaggatctg ggtgccaatc aggttctgct tgaggtgccc gaccaggaga
      421 ccgtatccca tattgttcag gtggcgtctg caagcaaggc acacattctt tccatcacac
      481 cacggaaaga gagtctcgaa gactatttcc ttcgagagat caaggaggaa tcccgataat
      541 gagaatctgg gcgatcgcgc tgaacacttt tcgcgaagcc acccgaaaca gggtcttcta
      601 catgatcctc atcttcatcg ttctcatgtt gctgagctcc gccgccttgg gaatgctctc
      661 tctcggggaa caggatcggg tggtaaagca tctgggactg tctgccatga acatttttgg
      721 actgatgctg gcaatgtttg tcggggtcgc tctggtacat gaagagttgg agaacaagac
      781 aatctatact ttaatgtctg cgggagtctc tcgcggacag tttctattcg ggaagtttct
      841 gggtctgaca atgaccatcg tggtcaacat caccatgatg actcttctac tcaccttgct
      901 gatattcatg actcctggtt cacacttgac agtaagcgtc tatcaggctg ccttgatgag
      961 tctcttcgaa atgatgatca tcattgccat tgccattctc ttctccgtcg tcacctcacc
     1021 ggtactggcg atgatcctga cctttctgat gtgggtcatc ggaatgatgt cgcagagcct
     1081 gcagttgttt gcgcttcgac ttggtgagct tggacactcc gtcgcaaagt atgtcatcct
     1141 tggaattctg ttgtttcttc cccgcctgga cgttttcaat ctgaaggacg agattgtcta
     1201 tagcgaggtt ctcgacttcc ggtatttgta catgttctat ggactgctct acacgggagc
     1261 ggtgctcgcg gtatcgaatt tcattttcag ccgaaaggac ttccagtaga tcatggcttg
     1321 ggctcgcaag aaagttgttc ggagcgtctt ggtctgcctt ctcctgctcg ccactctcgg
     1381 cacccagata ggcgtacagc aactccgatc cacccagagg gtcagagact atatctacct
     1441 tgttcctgat ctcaaggcgc tcaaggtcat ctcgctcagt ttccggagcc tcgtggcgga
     1501 catgctctgg cttcgcgcga tccagtactt tggtggctgg tattctcaga tgaagaagca
     1561 acccatgggt tttatccatc tgataaacgc tgtcgtgtat cttgatccaa gattcgttca
     1621 ggcctacagt tttggcgcat tctgtatcgc ggaaggtctg gatgactatg atgctgccgt
     1681 tgaactcctg gtacgggggg ctgagacgaa ttcggaaaac ccacaggcgt ggcgtctcat
     1741 gtttgatgca ggattcatcc ggttctacaa ccagaagcgc aacgaggaag ccaaagaact
     1801 tatcgatcgg gcatcccaga tgcctggcgc cgatgaattc acgaagagaa ccgcggccta
     1861 cattgacagt gtttctggcc gcagagacat tgcacgcgaa cgctacagag caatgtatga
     1921 aacagctgaa accgagttga tccgcgattt ggcacttaag catctcatcc ggattgaacg
     1981 ggatgaacac actgagattc tcaagtcggc tgtcgaagag tttaagcgca agatggagcg
     2041 taagccccgt gatctgaagg aattggtgga aaagggattt ctcaaagcaa tcccgtcaga
     2101 cccaggaggc tcccagtacg tttatgttgc caagtcggat gttgtgctcc tgaaagaggt
     2161 tcttgacatc gcgcgtgata ctctcgccgc agcgattaag actcaagcga ttgcttttga
     2221 ggaagctcgt ggcaggaaac ccctaagcct tcaggaactc attgatgaga gattcgtcgg
     2281 cgccgatctg accgatccag cgggaatgca catcgaactt gacctggaat cctattcgat
     2341 aatcttctcg gaaacacctg aatcatagag tccacctccg ctggtttgag ttgcatgggt
     2401 ccgcttgccg acacatgatt ctgatgctat cgttgtctaa gagcatagga gagaggcttc
     2461 tgaaacaccc atgaaaacgc tcccgaacca gatcgcagag tgg
//
LOCUS       DAOQUC010000529         2502 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_50696_length_2502_cov_2.583163, whole genome shotgun sequence.
ACCESSION   DAOQUC010000529 DAOQUC010000000
VERSION     DAOQUC010000529.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2502)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2502)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2502
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50696_length_2502_cov_2.583163"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            19..477
                     /locus_tag="PKH07_09645"
     CDS             19..477
                     /locus_tag="PKH07_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015750602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HNT35247.1"
                     /translation="MTAIKTKTSRMTRQKRIILEEISQLKSHPSASEVYEAVKKRLPK
                     ISMGTVYRNLQRLSSCGEIRALSPVGSSKRFDDNPDDHYHVRCMCCGRLVDVCLDSLD
                     DMEESVSDTTQFMVEGHFLEFYGVCPCCRQAEPSQRPHRKRAKASSGATS"
     gene            474..1289
                     /locus_tag="PKH07_09650"
     CDS             474..1289
                     /locus_tag="PKH07_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02757 family protein"
                     /protein_id="HNT35248.1"
                     /translation="MIQRKVKREALERLRRKYDSRDYVHPDPVEMLYPYQDSADREIV
                     GLIASCLAYGRVGQILKSAESLLDIMGSPRRFVEESTCSQMHHLLRGFKHRFTRADQI
                     VDLLCRVRAVLKEYGSLCECFVSGMSSQDDSLVPALTAFATKLGDSSAQGFSLVPSPL
                     DGSACKRLNLYLRWIVRKDNVDMGDWANAVPQSRLIIPLDTHMHWICRNLGLTDRKQA
                     DFRAAIEVTEGFRHFDAEDPVRYDFALSRLGIRERIKPQALWSVLAEQSEVSA"
     gene            1286..>2502
                     /locus_tag="PKH07_09655"
     CDS             1286..>2502
                     /locus_tag="PKH07_09655"
                     /inference="COORDINATES: protein motif:HMM:NF015715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HNT35249.1"
                     /translation="MTGHLMSAVAEFWGVLTEMSPYLLFGFLVAGILSIVISQKLVER
                     HLGGRGFLPVLKASLFGVPLPLCSCGVIPVAVSLRHQGATRAATTSFLLSTPQTGVDS
                     ILVTFSLLGPVFAVFRPLAAFVSGLLGGGLLIPFEKDDPAHGSSTEPCAESCCYNGDN
                     ALRGRLLHGLRHGFVTLPGDIYKSLALGIALAAIISSIVPDDFFHGVFGGGFASMLLM
                     MLVGLPLYVCATASVPIAAALIAKGVSPGTAFVFLTTGPATNAATIVTIWRTMGRRTA
                     VLYLLTVSVTAIGGGLILDQVYQTTGQQAMTHYHSMMPLWLKSVSALVLCAICGAAWI
                     RSWKTSAKPATLTPETGTVLQVSGMTCAHCAETVRKTLSACRGVSSVSVDLKQGLAQV
                     QGHELDFEQMRSQL"
BASE COUNT          506 a          641 c          723 g          632 t
ORIGIN      
        1 tagtagtcgg aggcggagat gactgcaata aagacaaaga cttcaagaat gactcgtcag
       61 aagaggatta tcctcgaaga gatttcccaa ctgaagtcac atccatccgc atccgaagtt
      121 tatgaggccg tcaagaagag acttccgaag atcagtatgg gtacggtcta tcgaaatttg
      181 caacgactct cgtcctgtgg agagatcagg gcattgtctc cagttggatc ctcaaaacgc
      241 ttcgatgaca atcccgacga tcactatcat gttcgatgta tgtgctgcgg acgcctggtt
      301 gatgtttgcc tggactccct ggatgatatg gaggagtcgg tctccgatac gacccaattt
      361 atggtcgaag ggcattttct ggagttttat ggtgtctgtc cttgctgtcg gcaggctgag
      421 ccgtcacaga ggcctcatcg gaagcgggca aaggccagtt cgggggcaac ttcatgatcc
      481 agcggaaagt gaaaagggaa gcattggaac ggctgaggcg gaagtacgac agccgcgact
      541 atgtgcatcc ggatcccgtc gagatgctct atccctacca ggattccgca gaccgtgaaa
      601 tcgtcggact cattgcctcg tgcctagcct atggccgtgt cggacagatt ctcaagagtg
      661 ctgaatcact gctggatatc atgggcagtc cccgtcgctt tgtcgaagag tccacatgca
      721 gtcagatgca tcatcttctg cgcggtttca aacaccggtt cactcgagcg gatcaaatcg
      781 tggatttgct ctgtcgagtg agggctgttc tcaaagagta tggctcattg tgcgaatgct
      841 ttgtaagtgg catgagctct caagatgaca gcctcgtgcc cgcactgaca gcctttgcca
      901 ccaaactggg cgactcctct gcccaaggtt tcagtctggt gccttcgcct ttggatggaa
      961 gtgcctgcaa gcgtctgaat ctctatctca ggtggattgt tcgaaaggat aatgtggaca
     1021 tgggcgactg ggcaaatgct gttcctcaaa gccgacttat cattcctttg gatacacaca
     1081 tgcactggat atgccggaat cttggtctga cagacagaaa gcaggcagac tttcgggcgg
     1141 cgattgaagt cacggaagga ttcaggcatt tcgatgcgga ggatcccgtt cggtatgact
     1201 ttgccctgag tcgccttggg attcgagaga gaatcaaacc acaggcgctc tggtcggtac
     1261 ttgctgagca aagtgaggtg tcggcatgac gggtcatctg atgtcggctg tggcggagtt
     1321 ctggggtgtc ttgacggaga tgtctcccta tctgctcttt ggattcctgg tcgctggcat
     1381 tttgtccatc gtgatatcgc agaaacttgt ggagaggcac cttgggggac ggggtttcct
     1441 tcccgttctg aaggcttcgc tctttggtgt tccacttcct ttgtgttcgt gtggggtgat
     1501 tcctgttgcg gtttcgttga gacaccaagg agccacacgg gcggcaacca cgagttttct
     1561 tctgtccacg ccgcagacgg gtgtagacag tattcttgtc accttcagtc tcctgggtcc
     1621 tgtgtttgca gtcttcaggc cattggccgc ctttgtgagt ggacttcttg gaggcggact
     1681 cttgatcccg tttgagaagg acgatcctgc tcacggttcc agcaccgaac cctgcgctga
     1741 gagctgctgc tacaacgggg acaacgccct cagaggacga ctgctccatg ggttgcgaca
     1801 tggctttgtg acattgccgg gggatatcta caaatcgctc gcactgggga tcgccctggc
     1861 ggcgatcatc tcctcgattg ttccggatga tttctttcac ggagtctttg ggggaggctt
     1921 tgcctcaatg cttctgatga tgctggtcgg tctgcccctc tatgtttgtg cgacggcttc
     1981 ggttccaatc gccgcggcac ttatcgctaa aggggtctct ccaggaaccg cgtttgtgtt
     2041 cctgacgacg ggtccggcaa ccaatgcggc aacgatcgtt acgatctggc gcaccatggg
     2101 acgacgcacg gcggttctct atctactcac cgtttcggtc acagcaatcg gtggcgggtt
     2161 gatcctcgac caagtctatc aaacgacggg acagcaggcg atgacgcact accacagcat
     2221 gatgccccta tggctcaagt ctgtctcagc cttggtgctc tgcgccatct gcggcgctgc
     2281 ctggattcgt tcatggaaga cgtccgccaa acctgctacg cttaccccag agacggggac
     2341 ggttttacag gtttccggga tgacctgcgc tcactgtgcc gagaccgtca gaaagacatt
     2401 gtccgcgtgc cgtggtgtgt cgtctgtgtc ggtggacttg aaacaggggc ttgctcaggt
     2461 gcaggggcat gagctcgact ttgaacagat gcgctctcaa ct
//
LOCUS       DAOQUC010000530         2501 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_50725_length_2501_cov_3.206868, whole genome shotgun sequence.
ACCESSION   DAOQUC010000530 DAOQUC010000000
VERSION     DAOQUC010000530.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2501
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50725_length_2501_cov_3.206868"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1138
                     /locus_tag="PKH07_09660"
     CDS             <1..1138
                     /locus_tag="PKH07_09660"
                     /inference="COORDINATES: protein motif:HMM:NF012622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dockerin type I domain-containing protein"
                     /protein_id="HNT35250.1"
                     /translation="LGSARKDQRVLFVAGKPMAFLKENGFKPLRDFIKETMQDRGIAG
                     DKMVCLSSLESNLQTVTAAINALQVAEELLVIVVGEGTRDGVKLGDGYVLTPQILGTI
                     AAQIPGMSIFIIDSDHSGIFGGPLVGSTLLGSRRIFFTSSGSAQKAWFTSGGLGSFTG
                     LFCANLRQSLNMGEAFEKVCNHFKIMGTFTGQTPQSFAVNTNSQDIVFGYAKGTETSD
                     LLPPVVVTLDAPSSILLGQSSVVSAQVLEASGVLSVTGTFTSGTTFAVQEESFTLISG
                     STDTYQSPPMLFDEVGNLVVSIIAMDIAGNVSDPTAALVPVYVRPEKGDLDGNGVVDW
                     KDLLVFQAAWTGSSSSAGYNHLADLNDDGIVDHKDLLLFVKRML"
     gene            1401..2219
                     /locus_tag="PKH07_09665"
     CDS             1401..2219
                     /locus_tag="PKH07_09665"
                     /inference="COORDINATES: protein motif:HMM:NF016816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HNT35251.1"
                     /translation="MLMTDLEGVAGVLNNHDWTSPDGRFNEKGMRLLTEEVNAAVDGL
                     FAGGAREVVIVDGHGSGAIDPEILDERAQLSRGRHEQVWPWGLDNTFHGLGFVGQHAK
                     AGTPYSHITHTQNMRYIDLSINGVSIGEYGQLALCAMELGIPTIFACGEQALAVEAEN
                     LTPGVITVAVKRGLLPDGLDHLSAEEYKKAKLSAIHVSPKKSRALIRHGAFKAAERLR
                     NDPRSFSYPKFAPPYVRTARFRTLEDKPAYQARDEHPNSITSLMNMPFTPLVTP"
BASE COUNT          574 a          653 c          679 g          595 t
ORIGIN      
        1 tcttggctcc gctcggaagg atcagcgggt tcttttcgta gctggcaagc ccatggcatt
       61 cctcaaggag aatggattca agccccttcg ggatttcatc aaggagacca tgcaagacag
      121 agggatcgct ggcgacaaga tggtctgtct gtcttcactc gagtccaacc tgcagacagt
      181 gactgcagcg atcaatgcgc tgcaggtggc tgaggaactc ctcgtcatcg tggttggcga
      241 aggcaccagg gacggcgtca agttgggcga tggctatgtg ctgacccctc aaattcttgg
      301 gacgatcgcc gcacaaattc cagggatgag tatcttcatc atagactctg accattcagg
      361 catcttcggg gggcccttgg tcggcagcac acttttggga agtagaagga tcttctttac
      421 cagttctggc tcggcacaga aggcctggtt tacgagtggt ggtttgggtt cattcacggg
      481 actcttctgc gccaaccttc gccagtcgct caacatgggc gaagcattcg aaaaggtctg
      541 caatcatttc aagatcatgg gaacgtttac cggacagact ccccagagtt ttgccgtcaa
      601 taccaactct caagatatcg tttttggcta cgccaaaggt accgagacaa gcgatttgct
      661 cccgccggtt gtcgtcacac tggatgcccc atcctcgatc ctgttgggac agagcagcgt
      721 cgtttcggcg caggtgttgg aagcatccgg tgtgctcagc gtcacgggaa ctttcaccag
      781 tggcaccacc ttcgccgtgc aggaggaatc ctttaccctc atatcaggtt ccacggatac
      841 gtatcaaagc ccccccatgc tcttcgatga agtcgggaac ctcgtcgtct ccatcatcgc
      901 gatggatatt gccggaaacg tctccgatcc gacagcggct ttggttccgg tctacgtccg
      961 tcctgagaaa ggcgacttgg atggcaatgg tgtcgttgac tggaaggatc tcctggtctt
     1021 tcaggcagca tggactggca gcagttcatc cgcaggctac aatcatctgg cggatctgaa
     1081 cgacgatgga atcgttgatc acaaggattt gcttctgttt gtcaagagaa tgctctgagt
     1141 tcttcctgcc ttctcggggc tttcaatagg tctctgccga atgcagagca tccaggagtt
     1201 ggcaacgagc cgaagggatt tgaatatcga agctgactct ttctgtcttg tcccaatctt
     1261 cggtaaaaat cgtttggact tgcctctgag tcttggagtg gtacagtaga ctcagcaagt
     1321 tgtcggacag attgccgagt ccgacgcacg tgtatctcga tttcgggtta ttctttggag
     1381 gaacgaccgc agtgaagata atgctcatga ctgacttgga aggcgttgcc ggagttctca
     1441 acaatcatga ctggacttcg cccgatggca gattcaatga gaagggaatg cgtcttctga
     1501 cagaagaagt caacgctgcc gttgacgggc tttttgcagg tggagcacgt gaagttgtca
     1561 ttgtggatgg ccatggctca ggagccattg atccggaaat tctggatgaa cgtgcccaac
     1621 tgtcgagggg gcgacatgaa caggtgtggc cgtggggact cgacaacacg ttccatggac
     1681 ttgggtttgt aggccaacac gccaaggcag gcacccccta ttcacacatt acacatacgc
     1741 agaacatgcg gtacatcgat ctctccatca acggcgtctc aattggagag tatgggcagc
     1801 tggctctctg tgccatggaa ctagggattc caacgatttt cgcctgtgga gaacaggcac
     1861 ttgcagttga ggcagaaaat ctgacacctg gggtgatcac agtcgccgta aagaggggct
     1921 tgctgccaga cggtctcgac cacctcagcg cggaagaata caagaaggcc aaactcagcg
     1981 cgattcatgt ctctccaaag aaatcccgcg cgttgatacg acacggtgcc ttcaaggctg
     2041 ctgagaggct tagaaacgat ccgagaagtt tctcataccc caagttcgcc cccccgtatg
     2101 tacgaactgc tcgatttcga acactggaag acaaacccgc ctatcaagca cgcgatgagc
     2161 atcccaacag catcaccagt ctgatgaata tgccattcac gccgctagta acaccgtaga
     2221 ctatagtttc acagacaaga tgcttgaact tccgaacaga tgagcacctg cgggcacggg
     2281 ggttataccc actcctgcaa acgcctgctc atcggaggaa gctgacttct tcaaagtctg
     2341 tttgttcatc cggatgatcg gcagtcccaa gttgggggtc actcgaatat ggtagtgaat
     2401 ctcatctgcg caaaaaaccg cgccgatttt tctcgggttg gggtgttggt ttcaactcga
     2461 cccgtgtgcc cgaggcagtt ccccaagtta cttggcctcc g
//
LOCUS       DAOQUC010000531         2500 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_50753_length_2500_cov_2.655624, whole genome shotgun sequence.
ACCESSION   DAOQUC010000531 DAOQUC010000000
VERSION     DAOQUC010000531.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2500
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50753_length_2500_cov_2.655624"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            110..985
                     /locus_tag="PKH07_09670"
     CDS             110..985
                     /locus_tag="PKH07_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HNT35252.1"
                     /translation="MLPKELISRIRQIEIRTKRFVDDVLAGEYHSVFKGRGMEFNEVR
                     EYTAGDDFRSIDWNVTARYGHPFVKRYVEERELTVILVVDVSGSERFGTKGQMKSELC
                     AELSALLAFSAIRNNDRVGLILFSGEVEKFVPPKKGRKHVLRLIREILYFQPSGATTS
                     ISNALQFMAQVQKRRCVAFLISDLIDEGYEKTLSIAARRYDLIALKISDPREREMPRI
                     GFLEVEDAETGETFLLDTQDREFQGRYQQLAEKREQSCVQMFNRIGLDHVPIQTGESY
                     IIPLVRFFQRRARER"
     gene            982..1989
                     /locus_tag="PKH07_09675"
     CDS             982..1989
                     /locus_tag="PKH07_09675"
                     /inference="COORDINATES: protein motif:HMM:NF025674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4381 family protein"
                     /protein_id="HNT35253.1"
                     /translation="MRGWNVTFRCQLSSIAIGLLLTFAAGLAMADGNSLPSPVEVTVQ
                     ARPMEITMGDRVHYQLDIVYPEGLSIQERKPLHELGDFEILDATAPTETHDKQGRKVL
                     SKAYSITAFATGTLSIPPYEVFYSDAQGVGGSVKSAPLTIAVKSLIGSGTPTLRDIKP
                     PATIPMQVSSWVWWILGALIALVVLVLLLGFWKKPQSVAEVLPPEFQIPADTWALQQL
                     RKIRLGPILKEKQTKALALAVSEIVRRYLDRRFQMNTIDMTTEEILDILKETPILREL
                     DSLFGDFLSLCDLIKFAKYQPPDEKLVSLLDQAETIVKETAPAHTTEQQPSETQEEGL
                     S"
     gene            1986..>2500
                     /locus_tag="PKH07_09680"
     CDS             1986..>2500
                     /locus_tag="PKH07_09680"
                     /inference="COORDINATES: protein motif:HMM:NF024909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNT35254.1"
                     /translation="MRFESPWYLIGLVLIPLLPVFRHYRIRKDRPRLRFSSVAALKAA
                     GSSPKAFLRFVPGLLRLSAMMLVVLALARPQSYIDIFKSYTEGLDIVLALDVSTSMRA
                     EDLAKGKNRLDVAKEVVDWFLSKRETDRVGMVVFAAVAFTQCPLTLDYGLVRQFLETL
                     ETGMVEDGTAIG"
BASE COUNT          567 a          645 c          703 g          585 t
ORIGIN      
        1 tgaagcggag gactacacgt cagacacggt agtccagaag gttttcgatc acgtcgaggt
       61 accttaggag tctgtcggaa gcggccaatc ctgatggagg ttcatgttca tgctacccaa
      121 ggaactgatc agcaggattc gtcagattga gattcgaacc aagcggtttg tggacgatgt
      181 ccttgcgggc gaataccaca gcgtgttcaa aggcaggggc atggagttca acgaggttcg
      241 cgagtatacc gcgggggatg attttcgctc gatagactgg aacgtcaccg cgcgctacgg
      301 acatcccttt gtcaaacgtt acgttgaaga aagagagttg accgtaatcc tggtggttga
      361 tgtgtccggc tcggaacgct ttggaacgaa agggcagatg aagtccgaac tgtgcgccga
      421 attgtccgcg ctgctggcgt tttctgcgat ccgcaacaac gatcgggtcg gactgatcct
      481 cttcagcggc gaggtcgaga agttcgtccc gcccaaaaag ggacgaaagc acgtcttgcg
      541 tctgattcgc gaaattctct atttccagcc ttctggtgcc accacgagca tttcaaatgc
      601 gctgcaattc atggcgcagg ttcagaaacg tcgctgtgtc gcattcctta tctccgatct
      661 gattgacgaa ggctatgaga agactctctc catcgccgca cgccgttacg acttgatcgc
      721 gctcaagatt tctgatccga gggaaaggga aatgccgcgc atcggtttcc ttgaagtcga
      781 agacgcagaa acgggagaga ctttccttct ggacactcag gatcgggagt ttcaaggccg
      841 ctatcagcag ctggccgaaa aacgtgagca gtcctgcgtt caaatgttca accgaatcgg
      901 tcttgaccat gtgccaatcc agacggggga atcgtatatc attcctctgg tgcgcttctt
      961 ccaaaggagg gcacgagagc gatgaggggt tggaacgtca catttcgatg tcaactttct
     1021 tcaatcgcca tcgggctgct tttgacgttc gcggctggtc tggcaatggc ggatgggaac
     1081 agtcttccat ctcccgtcga ggtgacggtg caggcacgcc ccatggagat caccatggga
     1141 gatcgagttc actatcagtt ggatatcgtc tatccggaag gtctcagtat tcaagagaga
     1201 aagcctctgc acgagttggg ggattttgag atcctggatg ccaccgcgcc aacggaaaca
     1261 cacgacaagc agggcagaaa ggttctgtca aaggcctatt cgataaccgc attcgcaacc
     1321 ggaaccctct ccatcccgcc atacgaggtc ttctacagtg atgctcaggg ggtgggaggc
     1381 tcggtgaagt ccgcacctct gacgattgcc gtcaagagtc tgatcggctc gggaactccc
     1441 acgctacggg acatcaagcc accagctaca atcccaatgc aagtctcctc atgggtatgg
     1501 tggattctgg gagccttgat cgcgctcgtg gttcttgtgc tgctcttggg attctggaag
     1561 aaaccacaat ctgtcgccga ggtgctccca cccgagtttc agattcctgc cgacacatgg
     1621 gctttgcagc aactgaggaa gattcgactt ggtccgattc tgaaggaaaa gcagaccaaa
     1681 gcacttgctc ttgctgtcag tgagatcgtc cgtcgctatc tcgacaggcg ttttcagatg
     1741 aacaccatag acatgaccac agaggagatt cttgacattc tgaaagaaac accaattctg
     1801 agagagttgg atagcctctt cggtgacttt ctttccctgt gtgacttgat taagtttgcc
     1861 aagtatcagc cgcctgatga gaagcttgtc tcgttgttgg atcaggcgga gaccatcgtc
     1921 aaggagaccg caccggcaca taccacggag cagcagcctt ccgaaacaca agaggagggc
     1981 ttgtcatgag gtttgaaagt ccctggtatc tgatcgggct ggttctaatc ccattgctgc
     2041 ccgtctttcg gcactaccgt atcaggaaag acaggccgcg actgcgcttt tcatcggttg
     2101 ctgccctcaa ggctgcggga tcctcaccca aggcattcct tcggtttgtt ccaggactgt
     2161 tgagactctc ggcgatgatg cttgtagttc tcgccctcgc tcgacctcag tcgtacatag
     2221 acatcttcaa atcatacact gaggggctgg atattgtcct tgcactggat gtttcgacca
     2281 gtatgagggc ggaagatctg gccaagggca agaaccgtct cgacgtcgcc aaggaagtcg
     2341 tggactggtt tctgagcaaa cgggagaccg atcgagttgg catggtcgtc ttcgccgccg
     2401 tcgcattcac tcagtgtccc ctcacgcttg actacggact ggttcgacag tttctggaaa
     2461 cgctggagac cggcatggtg gaggatggga cggccatcgg
//
LOCUS       DAOQUC010000532         2499 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_50768_length_2499_cov_3.584288, whole genome shotgun sequence.
ACCESSION   DAOQUC010000532 DAOQUC010000000
VERSION     DAOQUC010000532.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2499
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50768_length_2499_cov_3.584288"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(24..971)
                     /locus_tag="PKH07_09685"
     CDS             complement(24..971)
                     /locus_tag="PKH07_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007360426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNT35255.1"
                     /translation="MSTPRVGIIGCGGIAYAHRDGYRANGIEPIAFMDTNKAAAEALA
                     KDVKNAKVFDTAQALIESGMVNVVSVCSPPAFHEEATVLSLRNGIHTLCEKPLAHTIE
                     SGKNIAAAAASSSALCMTAFRHRFLASMVKIRSLLDEDRIGSVVFFNNIFCGHAPGMK
                     DKWFSKKALAGGGSMLDTSSHSADLFRFLVGEVAEQKAVTNRRMENADVEDSAVLIVK
                     ADNGAIGSMTSSWMAGTGEAYVDIMGEKGRIFYDYIKGTEVRVELLNGEPEVHNVEVT
                     WGFKEEIEHFLGAIEGKYPLACTAHDGLRSLEIIMRCYS"
     gene            complement(968..2359)
                     /locus_tag="PKH07_09690"
     CDS             complement(968..2359)
                     /locus_tag="PKH07_09690"
                     /inference="COORDINATES: protein motif:HMM:NF024120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HNT35256.1"
                     /translation="MLKFLLLLLVFAGCVSFSSRQSVTPSQTTSRGFDVREFGAQGDG
                     MTDDTQAFQDTLDRCAESGGGVVSVPAGKYLIKTNILVPPSVTLEGTWRAPSSVDRYH
                     RVPQEPDKGPELQGSVLLAVENPGLEDATAFIRLERNATLKGVTIFYPHQTKTNPPVA
                     YPWTVATVGADNCSIIDVLMVNPYQAVDFGNRASGRHYIRSLYAQPLRRGIYVDHCLD
                     VGRLENIHLWPFWTAADPDSPVGRFMLEQGEAFIFGRSDWEYVSNCFAIAYKIGFRFI
                     KGKGEGPFAGGGNYLLSQSGADMCDMAVLAEDIQAHSGVSFSNSQIFGDIITQETNHG
                     MLRFSSCGLFGSISGRNGVGLARIAGRGRVSFDNCHFYAIDRRIETDRLIHVVEGRVS
                     ITDSVFINSWDAPYNHQPIVLERPVRAAIITNNEFYGRAGIVNNAIGRVVIEGNVENT
                     DIEPFPNKGEEVK"
     gene            complement(2375..>2499)
                     /locus_tag="PKH07_09695"
     CDS             complement(2375..>2499)
                     /locus_tag="PKH07_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome C assembly protein"
                     /protein_id="HNT35257.1"
                     /translation="VRGWRGRRATRWLGAGAVLLLLAYVGSRFVFEVLVGRVAG"
BASE COUNT          555 a          720 c          670 g          554 t
ORIGIN      
        1 cagctgagga aatcaagaac tcatcaggaa taacaacgca tgatgatctc cagcgaccgc
       61 agcccgtcgt gtgcagtgca ggcgagagga tatttccctt cgatggcacc gagaaagtgt
      121 tctatttcct ctttgaagcc ccaagtcacc tcgacgttgt ggacttccgg ctcaccgttc
      181 aacaactcga cgcggacttc ggtgcctttg atgtagtcgt agaagatgcg tcctttctcg
      241 cccatgatat ccacataggc ctcgccggta ccagccatcc aggatgaagt catcgagccg
      301 attgcgccat tgtccgcttt gacgatcagc actgccgaat cctcgacatc cgcgttctcc
      361 atacgtcggt ttgtgactgc tttctgttcg gcaacttcgc caaccaaaaa ccgaaagaga
      421 tcggcagagt ggctactggt atccagcatc gaaccaccgc ccgcaagcgc ttttttggag
      481 aaccacttgt ctttcatccc aggcgcatgg ccgcagaata tgttgttgaa gaagacaacg
      541 gatccaattc tatcttcatc aagaagggaa cggattttga ccatcgaggc cagaaagcgg
      601 tgtcgaaagg ccgtcatgca aagagcgctg gatgaggccg ctgcggccgc aatgttcttc
      661 cccgattcga ttgtatgcgc aagtggtttc tcgcagagtg tgtggatgcc gtttctcagg
      721 ctcagcacgg tcgcctcttc atgaaaagcg ggtggcgagc acacgctcac gacattgacc
      781 atgccagatt cgatgagagc ctgcgcggtg tcaaagacct tggcgttctt gacgtctttt
      841 gccagggctt ccgccgctgc cttgttggtg tccatgaaag cgattggctc gattccattg
      901 gcgcgatacc catctcgatg ggcataggcg atgcccccgc atccgatgat tccgactctg
      961 ggcgtgctca ttttacttct tctcctttat tgggaaacgg ttcgatgtcc gtgttttcga
     1021 cgttgccctc gatcacaaca cgaccgatgg cgttgttgac gatgcctgct ctgccgtaga
     1081 attcgttgtt ggtgatgatg gctgcacgca cggggcgctc cagcacgatg ggctgatgat
     1141 tatacggcgc gtcccaactg ttgatgaaga ccgaatccgt aatgcttacc cgtccctcaa
     1201 ccacatggat cagccgatcg gtttcgattc gtcggtcaat ggcatagaag tgacagttgt
     1261 caaatgaaac acgacctctg ccggcaattc gtgccagacc gacaccgttt ctgcctgaga
     1321 tcgagccaaa cagaccgcaa ctcgagaagc gcagcatccc atgattggtc tcctgcgtga
     1381 tgatgtctcc aaagatctgc gaattggaaa aagacactcc cgagtgcgct tggatatcct
     1441 cagccagaac agccatatca cacatgtctg ctccgctctg ggacagcagg tagttgccac
     1501 cgcctgcaaa ggggccttct ccttttccct tgataaatcg aaagccaatc ttgtacgcaa
     1561 tggcgaagca gttactcaca tactcccagt cacttcgacc gaagatgaat gcttcgccct
     1621 gctccagcat gaaccttcca accggactgt ccggatcggc agcagtccag aacggccaga
     1681 gatgaatgtt ctccagacgt ccgacgtcga ggcagtgatc aacgtagatt ccccggcgca
     1741 gcggctgggc atacagactg cggatgtaat gccgaccgga tgctcgattt ccaaagtcaa
     1801 ccgcctgata gggattgacc atcaagacat caatgatgga acagttgtct gctccgacgg
     1861 tggcgactgt ccaggggtag gcgacagggg gatttgtttt tgtctgatgg ggatagaaga
     1921 tggttactcc cttcagcgtt gcatttctct ccagtcgtat gaatgctgtg gcatcttcca
     1981 gtccaggatt ctcgactgcc agcaagacac tgccctgcag ttccggcccc ttgtccggtt
     2041 cctgtgggac gcgatgatag cgatccactg atgaaggagc gcgccatgtt ccttcaagcg
     2101 tcacagaggg aggcaccagg atatttgttt tgatcaggta tttccctgca gggacggaaa
     2161 ctacaccgcc gccggattct gcgcagcgat ctaacgtatc ctgaaacgct tgggtgtcgt
     2221 ctgtcattcc gtcgccctgg gcaccaaact cccgaacgtc aaaaccgcgg ctggtggttt
     2281 gggaaggagt cacagattgt ctggaagaaa aggagacgca gccggcaaac accagcagca
     2341 ggagcaggaa tttcagcatc ggcgcagccc ctcgtcagcc ggccacgcgg ccgaccagca
     2401 cctcgaagac gaagcgcgag ccgacgtagg ccagcagcag cagcaccgcg ccggcgccca
     2461 gccagcgcgt ggcccgccgc ccgcgccagc cgcgcacct
//
LOCUS       DAOQUC010000533         2499 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_50769_length_2499_cov_3.405074, whole genome shotgun sequence.
ACCESSION   DAOQUC010000533 DAOQUC010000000
VERSION     DAOQUC010000533.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2499
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50769_length_2499_cov_3.405074"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            82..2325
                     /locus_tag="PKH07_09700"
     CDS             82..2325
                     /locus_tag="PKH07_09700"
                     /inference="COORDINATES: protein motif:HMM:NF017263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HNT35258.1"
                     /translation="MKRVVALVGLLSLALLFSCGRSQAIKAESNKSNPSERTSSVKEE
                     RTKSTMSTIEFPDHPRLLLNAREIELIKERIAKHDWAQAYWKQVQERADTVLEQSVVL
                     PPRGGNWWHWYACPEHGGRLRTGKRIGDWEWEHTCSVGGEVFRGDTSAPKTDYDGCTL
                     QGEHDGWSKVVLDLGLAYAISGRKDYANKAREILLAYADRYSTYPLHTIYGEDKIGGG
                     RIGAQTLDESVWLIPVCQGADLVWDTLTEEDRKHLAEGLLLPAVTEVILPHKLGIHNI
                     QCWKNSAVGLVGFLLGDRKLVQNAIENPERGFRQQMAKGVLPDGLWWEGAWGYHFYTM
                     MSLWSLTEAARNCGIDLYEPAFKRMYDGPIRFAMPNLLLPAFNDSGTASVKGSASHYE
                     LAFRRFRDPEYCAVLETSNRITDKAFLYGEMSLPSTGARQRESVNYPESGYAILQRGQ
                     GEGATWLCLKYGPHGGGHGHPDKLSFVLCSKGEILGVDPGTAAYGLPIQNGWYKTSIA
                     HNTLVVDGNSQAPAQGKCLAFGTESGVDYAQAEAGAIYDGVKFHRTVALVEEDLLVFL
                     DQVESATEHTYDLAYHQSGAWAGTVSDQPFILEEKPGYAYLQDAVVRRVSEPAFLKVE
                     TRGKLSVNIGFLPEQPTEVITATGVGAHLNDRVPVAIFRRRASNACFVWTVVIGRDEP
                     SVRLLRTEDLEGNAVPGHVAAAVVVKREQGSLLVVANPSKDYLIVTLPDGTRQRTDQS
                     VFVRALQ"
BASE COUNT          573 a          633 c          749 g          544 t
ORIGIN      
        1 tctgctcccc ggaattgtca tttgaaaggg gagcgcggac gccatgaaat ggcttgcgga
       61 taggcatcag gaggttggct cgtgaaaaga gttgtcgctc tggttgggtt gttgtctctg
      121 gcgttgcttt tttcatgtgg gagatcccaa gccatcaagg cagagagcaa caagagcaat
      181 ccatccgaaa gaacatcttc ggtcaaagag gaaaggacta agagcaccat gagcaccatt
      241 gagtttccag atcatcccag actcctgttg aacgccaggg agattgagtt gatcaaggag
      301 cgaattgcca aacatgattg ggctcaggcc tactggaagc aggtgcagga acgagcggat
      361 acggttctgg aacagagtgt cgttctgcct ccgcgtggcg gcaactggtg gcattggtac
      421 gcctgtcccg aacacggagg caggctccga acgggaaaga gaatcggcga ctgggaatgg
      481 gagcatacct gttccgtggg tggagaggtt ttccgaggcg acacgagcgc acccaagacg
      541 gactacgatg gatgcacgct tcagggagaa cacgacggtt ggagtaaggt agttctggac
      601 ttgggtctgg cctacgcgat cagtggcagg aaggactatg cgaacaaggc acgggagatc
      661 ttacttgctt atgccgaccg gtattcgacc tatccgcttc ataccatcta tggcgaagac
      721 aagattggtg gcggtcgcat cggtgcgcaa acgctggatg agtcggtctg gctcattcca
      781 gtctgtcagg gagctgacct cgtgtgggac actttgactg aagaggatcg gaagcacttg
      841 gccgaagggc tccttcttcc cgccgtgaca gaagtgattc ttccccacaa acttggcatc
      901 cataacatcc aatgctggaa gaactccgcc gtaggcttgg tcggttttct gctgggagac
      961 aggaagttgg ttcagaatgc catcgagaat cccgagagag gatttcgcca acagatggca
     1021 aagggtgtac ttccagacgg actgtggtgg gaaggtgcct ggggctatca cttctacacg
     1081 atgatgtcgc tgtggagcct gactgaagcg gcgaggaatt gtggcattga tctctacgag
     1141 cctgcgttca agaggatgta cgatggtccc atccgtttcg ccatgcccaa cctgcttctg
     1201 cctgccttca atgacagcgg cacggcaagc gtcaaaggaa gtgccagtca ctatgaactg
     1261 gcgtttcggc gatttcggga tcccgagtac tgtgccgttc ttgaaacatc caaccgaatt
     1321 acggacaagg cctttctcta cggagagatg agtcttcctt caaccggagc caggcagcgc
     1381 gaaagcgtga actaccccga gtccggatat gccattcttc agcgaggcca gggagaaggg
     1441 gcaacgtggc tgtgcctcaa gtacggtcct catggcggtg gacatggtca tccggacaaa
     1501 ctcagctttg tgctctgctc caagggagag attcttggcg ttgacccggg aaccgcagcc
     1561 tacggtctcc cgattcagaa cggttggtac aagacaagca tcgcgcacaa tacactggtc
     1621 gtagacggaa acagtcaggc tcctgcccaa ggaaagtgcc ttgcatttgg caccgagtca
     1681 ggcgtggact atgcacaggc tgaggcgggg gcgatctatg atggcgtcaa atttcaccgg
     1741 acagtggcgc ttgtcgaaga agatcttctg gtgtttctcg atcaagtcga gagcgcaact
     1801 gagcatacat acgatcttgc ctatcatcag tcgggagcct gggcaggcac ggtatcagac
     1861 caacccttta tcctggagga aaagcccggg tacgcttacc tccaagatgc cgttgtgcgc
     1921 cgtgtgtccg aacctgcttt cctgaaggtt gagacaagag gcaaactgtc cgtgaacata
     1981 ggctttctgc cggagcaacc aactgaagtc atcacggcaa caggtgttgg cgcacatctg
     2041 aatgaccgcg tgcccgtggc aatcttccgc cgacgcgcat cgaatgcctg tttcgtttgg
     2101 actgttgtca tcggacgcga cgagccatcc gttcgtctgc ttcggacaga agaccttgag
     2161 ggaaacgcag tccccgggca tgttgccgcg gctgttgtcg ttaagcgaga acagggttct
     2221 ctcctcgtgg tcgccaaccc gtcgaaggat tatctgatcg tgacgttgcc cgatggcact
     2281 cggcagcgaa ccgatcagtc tgtcttcgta agggcacttc aatgacggtt gaactgattc
     2341 aggacgctct gaacaagacc catgatgtgc ttccacgaga acgatgggag agtttggggg
     2401 ctgtcagcat ctttctcagt aactggcttg tgagccggta cagggcacag cacgggcaag
     2461 tggacgcagc cttcattctg aagaactccc cagttcgtg
//
LOCUS       DAOQUC010000534         2498 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_50801_length_2498_cov_4.081867, whole genome shotgun sequence.
ACCESSION   DAOQUC010000534 DAOQUC010000000
VERSION     DAOQUC010000534.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2498)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2498)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2498
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50801_length_2498_cov_4.081867"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="PKH07_09705"
     CDS             complement(<1..330)
                     /locus_tag="PKH07_09705"
                     /inference="COORDINATES: protein motif:HMM:NF013355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HNT35259.1"
                     /translation="MKSAQSTVDLSVKVGDVALRNPIMTASGTSGCGEELSRFIELSS
                     LGAFVTKGVTLEPRQGNAPPRIVETCGGMLNAIGLQNVGLECFLQEQLPRLKDCGTPV
                     IVNIAGNT"
     gene            complement(421..753)
                     /gene="bamE"
                     /locus_tag="PKH07_09710"
     CDS             complement(421..753)
                     /gene="bamE"
                     /locus_tag="PKH07_09710"
                     /inference="COORDINATES: protein motif:HMM:NF016258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HNT35260.1"
                     /translation="MKSRRQRLVLCVAILPMMCFLLGCFTMGKEFQEDSVRAIQQGKT
                     SKNNVLNMFGAPGSKGIENGEETWTYSHYQFRLLSFETWAKDLTIRFDKAGIVNSYSY
                     SSSWPPTR"
     gene            complement(958..2406)
                     /locus_tag="PKH07_09715"
     CDS             complement(958..2406)
                     /locus_tag="PKH07_09715"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNT35261.1"
                     /translation="MRVFLGNSPWSKPGLYGVRAGSRWPHFERCGDPYMPFPFYLAYA
                     TAVLEKAGHDVLLLDGIAERISDDTFFERMQHFKPDLVLLEVSTPSIGKDLGDARRVK
                     ETLGKSVPIVFAGPYIEMYRSGFLEAQQDVDFVLMGEYEYTLRDLVEAYSTGKSWLGI
                     AGLIGRGSSGQVVVGERRPLIENLDELPWPARHHLPMASYHDCPGGIPCPSLQMWASR
                     GCPYKCIFCAWPQIMYHGSRYRVRNPKDVVDEIEHCVREYGIQSLYFDDDTFNIGKKR
                     ILEICDELKSRRLGLPWAVMARADTADLEMLQAMKGAGLQSIKYGVESASQKLVDACG
                     KALDLKKVEQIVKHTRNLGINFHLTFTFGLPGETRETIRQTIRYAKKLSPDSIQFSIV
                     TPFPGSAYFDMLDKQGFLLSKDWTQYDGSNQAVIRTENLSKEQLEEGLRMAYREWDRH
                     KFWRTFRQGKYLKKGMLHPVKALRYIRRHLGA"
BASE COUNT          585 a          725 c          607 g          581 t
ORIGIN      
        1 ggtgttgccc gcgatattca cgatgaccgg tgttccgcag tccttcaggc ggggaagctg
       61 ttcctggaga aagcactcca aaccgacatt ctgaaggccg atggcgttga gcatgccacc
      121 gcaggtttcg acgattctcg gtggcgcatt gccctgtcga ggttccagag tgacgccttt
      181 ggtgacaaac gcaccgagcg atgacagttc gatgaaacgg gagagttctt ccccacagcc
      241 ggaagttccg gatgccgtca tgataggatt gcgaagtgcc acatccccaa ctttgacgga
      301 cagatccacc gtggattgtg cgctcttcat gcagtcccct cttgatcccc ctgggtcgaa
      361 gggggacgag aatgatggaa cggctccaac caacgacgac cagaaacaat catgcaggca
      421 ctatctcgtt ggaggccagg aactggagta actgtaggag ttgacaattc cggctttgtc
      481 aaaccggatg gtcaagtcct tcgcccaagt ctcaaagctg agcaggcgaa actggtagtg
      541 gctgtacgtc caggtttcct cgccgttctc aatgcccttg gaacccgggg caccaaacat
      601 attgagaaca ttattctttg acgtcttgcc ctgctgaatg gctcgaaccg aatcttcctg
      661 gaactccttt cccatggtga aacatccaag taggaaacac atcatgggca aaatcgccac
      721 acacaatacc aaccgttgcc gtctgctttt catcccaatc atctctccct ttccaagact
      781 gtttttgtgt agattctagc aaacctgttc atcaatttca ctctttgatc ttgccggaca
      841 ctcgcgccag tacatacatc acccctgtga cgagcgtcag ccagagtaaa ttcagcgcct
      901 tccattttcg tcgtctcagc atggctggtc tccctcgaac gagacatcca caaaccacta
      961 cgcgcccagg tgccttcgga tgtatcgcaa cgccttcacc ggatgcagca tccccttctt
     1021 gagatacttt ccctgccgga aagtccgcca aaatttgtgc ctgtcccatt cccggtaggc
     1081 cattcgaagc ccttcctcga gttgctcctt ggacagattc tcagttcgga tcaccgcctg
     1141 attgctgccg tcgtattgag tccagtcctt gctcagcaga aagccctgct tgtcgagcat
     1201 gtcaaaatag gcacttccag ggaacggcgt cacgatggaa aactggatcg aatccggact
     1261 gagtttcttg gcatatcgta tcgtctgtcg gatggtttct cgggtctcac ctggaagtcc
     1321 gaacgtaaat gtcagatgga agttgatgcc aaggtttctg gtgtgcttga caatctgttc
     1381 cactttcttc aaatccagtg ctttcccgca ggcgtccacc aacttttggc tggcggattc
     1441 gactccgtac ttgatggact gaagtcccgc acctttcatc gcttgaagca tttcaagatc
     1501 agcggtgtcc gcgcgagcca tcaccgccca gggcagtccc agacgccgtg acttaagctc
     1561 gtcacaaatc tcaagaattc tcttcttgcc tatgttgaat gtatcgtcgt caaagtaaag
     1621 tgactggata ccgtactccc gaacacaatg ctctatctcg tccacaacat ccttgggatt
     1681 gcgcacccga taccgcgaac cgtgatacat gatctgcggc caggcgcaga agatgcactt
     1741 atacggacaa ccacggcttg cccacatttg aaggctcgga caggggattc caccgggaca
     1801 gtcatggtag gacgccatcg gtagatgatg ccgggcaggc cagggtagct cgtccaggtt
     1861 ttcgatcaaa ggtcgccgct cccccacgac aacctgtccg gatgatcccc gaccgatgag
     1921 accagcaatt cccaaccagg actttcctgt cgaataagct tcaacaagat ctcgaagcgt
     1981 gtattcatat tcccccatca ggacaaaatc cacgtcctgc tgcgcttcga ggaatccaga
     2041 acgatacatc tcgatgtacg ggccggcaaa gacgatggga acgctcttcc cgagcgtctc
     2101 cttcacgcgt cgggcatcgc ccaagtcttt cccaatggat ggagtggaaa cttcgagaag
     2161 caccaaatcc ggtttgaagt gctgcatacg ttcgaagaat gtgtcatcgg agatccgttc
     2221 tgcaatgcca tccagcagca gcacatcatg acctgccttt tcgagtacgg ccgtggcata
     2281 cgccaggtag aaagggaaag gcatatacgg atcaccacag cgttcaaaat gcggccatcg
     2341 ggatccggca cgaaccccgt aaagccccgg tttgctccaa ggcgaattgc ccaaaaagac
     2401 tcgcatcttc agaccgtcct gactcaggct gttgtcaaca gcctcggcat ttcagcgcgt
     2461 tccttggaaa agtagaacga tccccatttc ttgtcaaa
//
LOCUS       DAOQUC010000535         2496 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_50878_length_2496_cov_3.199508, whole genome shotgun sequence.
ACCESSION   DAOQUC010000535 DAOQUC010000000
VERSION     DAOQUC010000535.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2496)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2496)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2496
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50878_length_2496_cov_3.199508"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1009
                     /locus_tag="PKH07_09720"
     CDS             <1..1009
                     /locus_tag="PKH07_09720"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HNT35262.1"
                     /translation="RMQEPSAEPMTLDTIFDMASCTKATSTASAVMLLVQDGKIGLDD
                     PVAKHVPEFTHKDKDSITILSLLTHCSGLPAYTNAQYLEEHYGPRPKPDALIQRISEL
                     PVQGSVGEKYIYSCLNYLVLARVVQNVCGENMSDFLRERLWSPLGMKETTFYLNEKQL
                     AQTAPTIYEKGTLRRGLVHDPLAYYSVCEKYAPGNAGMFSTAEDLSRYVRMILKGGTW
                     GRRTVFTPDTWARITTNQSPSTLDTKRSCGWGIWTENAYATPLNNSPETAVLGHTGYT
                     GTLIWMDKLSKCYVIFLSNCVYPEDKSETKDAVIQGRRRIISVLLDHLEMYRGVRNES
                     K"
     gene            1056..1325
                     /locus_tag="PKH07_09725"
     CDS             1056..1325
                     /locus_tag="PKH07_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HNT35263.1"
                     /translation="MRAIVTVIGVDRMGIIAGVSQILAELRVNVLDISQTVLQEYFAM
                     IMLADLSQMTCSFAELSAKLNDEGHLLGVSIQVQREEVFKAMHQV"
     gene            complement(1354..>2496)
                     /locus_tag="PKH07_09730"
     CDS             complement(1354..>2496)
                     /locus_tag="PKH07_09730"
                     /inference="COORDINATES: protein motif:HMM:NF012551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HNT35264.1"
                     /translation="DPRVKELTDPAIWAHRMGEMHIQTEPNAEVHVQQTRHDFGFGTA
                     LSTGMFKQDADESVRAKYFQLLIDNFNCAVIENAFKWHQAEPYQNDLHWTTVDAMLDF
                     CDQHEMPLRGHCLFWAAEKYVPRWVKDISDEALREAIANRIRVTCERYRDRIGDYDVN
                     NEMIDHHYFENRLGEEIHDEMFRMAHEVDPKATLYLNDYSILDGSHLDAYEKQIRGFL
                     QRKVPVGGIGCQGHFLSPTFPSYERIMESLDRLARFGLPIKVTEFDREVDDEQQHARD
                     LDAFFRICFSHPSVRAILQWGFWEGAHWKPKAALYRKDFSPKPAAEAYRNLVFNEWWS
                     DCKATADSGGKCTVRVFYGHYTIETDGKTFSVDFPHQEKRKWLDLR"
BASE COUNT          575 a          654 c          664 g          603 t
ORIGIN      
        1 ccggatgcaa gagccttccg ctgagccgat gacgctggat acgatcttcg atatggcctc
       61 atgcaccaag gcgacctcaa cagcctcagc ggtcatgctg ttggtgcagg acggaaagat
      121 tggactcgat gacccagtgg ccaagcacgt tccggaattc acgcacaaag acaaagactc
      181 gattaccatc ctctctcttc tgacgcactg ctctggtctg cccgcctaca caaacgccca
      241 gtatcttgaa gaacactacg gaccacgtcc gaagcctgat gctctgattc agcgtatctc
      301 cgaactgccc gttcagggat ctgtcgggga gaagtacatt tacagctgtt tgaactatct
      361 ggttctggca cgtgtggtgc agaacgtttg cggtgagaac atgtccgatt ttctgcgcga
      421 acgtctttgg tctccacttg ggatgaagga aacgaccttc tacctcaacg agaaacaact
      481 ggcacaaact gcaccgacga tctatgagaa gggaactctg cgccgtgggt tggttcatga
      541 tccattggcg tactactcag tatgcgagaa atatgcgcct ggaaatgccg gaatgttcag
      601 cacagcagag gatttgagtc gctacgtgcg catgattcta aaagggggaa cctggggacg
      661 tcgaactgta ttcacgccgg atacatgggc gcgaatcacg accaatcagt ctccaagcac
      721 tctggacacg aagcgttcct gtggttgggg aatctggact gagaacgcct atgcgacccc
      781 gctgaacaac agcccggaga cggctgtcct gggccacacc gggtacactg gaacccttat
      841 ctggatggac aaactcagca agtgctacgt tatttttctc agcaactgcg tctatcctga
      901 ggacaagagt gagaccaagg atgctgtgat tcaggggcgt cgtcggatca tctctgttct
      961 cctggatcat ctggagatgt accgcggggt caggaacgag agcaagtgat ctggctctac
     1021 ggtgagtcag actatcccaa aggaacagta gcgtcatgag agcaattgtc accgttatcg
     1081 gcgtggacag gatgggaatt atcgctggtg tgagtcagat ccttgctgaa ctcagggtca
     1141 acgttctcga catcagtcag accgtgcttc aggagtactt tgcgatgatc atgctggcgg
     1201 atctttccca gatgacctgc tcgttcgcgg aactgtccgc caaactcaac gacgaaggcc
     1261 atcttcttgg ggtttccatc caggtgcaac gggaagaagt cttcaaagcc atgcaccagg
     1321 tctagactgt tgctgtctcg tgtttgcctg tggtcatctc aagtccaacc acttccgctt
     1381 ttcctggtga gggaagtcaa ctgagaacgt cttgccatct gtctcgatgg tgtaatgtcc
     1441 atagaagact cggactgtgc acttgccgcc gctgtcagct gttgctttgc agtccgacca
     1501 ccactcattg aaaacgagat tcctgtaagc ttcggcagcc ggtttgggtg agaaatcctt
     1561 gcggtacagc gccgctttgg gtttccaatg tgctccttcc cagaagcccc attggagaat
     1621 cgccctgacc gaggggtggg agaaacagat cctgaagaaa gcatccagat ctctcgcgtg
     1681 ttgttgttca tcatccacct cgcgatcgaa ctcagtgact ttgatcggga gtccgaagcg
     1741 tgccaggcgg tcgagactct ccatgattcg ttcgtaactc gggaaggttg gactgaggaa
     1801 gtgcccctga cagccaatcc cgccgacagg taccttgcgt tgcagaaaac cgcgaatctg
     1861 cttctcatag gcgtcaagat ggcttccatc aagaatagag taatcgttta gatagagcgt
     1921 tgcctttgga tcaacttcat gagccatgcg gaacatttca tcatgtattt cctcgccgag
     1981 acggttctcg aagtagtgat ggtcgatcat ctcgttgttg acatcgtaat ccccgattcg
     2041 atcccgatac cgttcgcagg tcactcgaat gcgattcgcg attgcctctc gcaacgcttc
     2101 atcggagata tccttgaccc atcgaggaac atacttctcc gctgcccaaa acagacagtg
     2161 accacgcagc ggcatttcat gctgatcgca gaagtcaagc atagcgtcaa cggtagtcca
     2221 gtgaagatcg ttttggtacg gttcggcctg atgccatttg aaggcatttt cgatgacggc
     2281 acagttgaag ttgtctatga gcagttgaaa atacttggcg cgcacggact cgtctgcatc
     2341 ttgcttgaac attccggtgc tcaaggcggt gccaaaaccg aaatcatggc gagtctgctg
     2401 tacatgcact tcggcgtttg gctcggtctg gatgtgcatt tctcccatgc gatgcgccca
     2461 aatggcaggg tcagtcagct ctttgactct tggatc
//
LOCUS       DAOQUC010000536         2496 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_50880_length_2496_cov_3.137239, whole genome shotgun sequence.
ACCESSION   DAOQUC010000536 DAOQUC010000000
VERSION     DAOQUC010000536.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2496)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2496)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2496
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50880_length_2496_cov_3.137239"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..406)
                     /locus_tag="PKH07_09735"
     CDS             complement(<1..406)
                     /locus_tag="PKH07_09735"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNT35265.1"
                     /translation="MTHPDTQPKVPILFIASGSSAGGAEVHLARLVRELAHGAYRITV
                     LCKQGSFLERTLTGQSVDIWTADIPHWRKLGGRLRMPFFISRMTAKAKRASIALVVSN
                     DFRTAPHALSIAEKMGIPTVSMIQDSIVDAKKS"
     gene            complement(403..1419)
                     /locus_tag="PKH07_09740"
     CDS             complement(403..1419)
                     /locus_tag="PKH07_09740"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNT35266.1"
                     /translation="MNETLSRIRDYLEWTVLRLSWSYSRLVPSWAVDRTISFLSTLTC
                     AFSTGMREQSLDKYAKLLMSEPGGMGTDKAISAERVSSVRVPSVFQCECLTEKLVGSF
                     LRNLLELDWIQRMVHEGPREFVTFDHLERLDAALAAGRGAVLATVHLGNWELVGSALS
                     LLGYPLRGVAWTARNRFIQKHLENLRSQTGLVTIFPDASAQIRIYRDLKANRPIAFVL
                     DLGQWENGVPVRFCGNDYLFPKGPLFFAQRTGAALIPSVSYRDETGRIHLLFEKPLDV
                     PSQGASSIETEKAMQTLATVLEGRVRQHPGQWLWVPAAKHQIAEMRRRDESMGALEQS
                     SENR"
     gene            1682..>2496
                     /locus_tag="PKH07_09745"
     CDS             1682..>2496
                     /locus_tag="PKH07_09745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35267.1"
                     /translation="MIRILPEFGVACCVIIVCLSARICLADQGDELPKPAYIVLDPNA
                     ARPPFAEGEPVPQLMKPPQAPRRTFGVPTIRSTSTAEGERIELDNEVINITLTVDCHR
                     RVLISSIRTGNQREILSAPASPFVVVDPEGRVTRDASDISVKSWMPRQHSIYTEIEIR
                     LTAPEPVLWRVRLYRNKPFLEQQISVPVHWRDRGCAVAQILPTGEMLKPVMPNNSFGR
                     GFSEGKPNLKGMHRFEFVSQSDHLCYDPESVSGVAAFVAGIGGEERIQQGHPPL"
BASE COUNT          597 a          693 c          647 g          559 t
ORIGIN      
        1 gagatttctt ggcatccaca atactgtcct ggatcatcga gaccgttgga atgcccattt
       61 tctctgctat agaaagggcg tgtggcgcag tccgaaaatc atttgagaca accaaggcga
      121 tggaagccct ttttgccttt gctgtcattc gagaaatgaa gaagggcatc ctcaggcgtc
      181 caccgagctt tctccaatgt ggaatgtccg ccgtccagat atcaacagat tgtccggtga
      241 gcgttctctc gagaaaactt ccttgcttgc agagcacagt aatccgatag gcaccgtgag
      301 caagttcgcg aaccagacgt gcgagatgaa cttcggcacc acccgcagaa gagcctgagg
      361 cgatgaacag gatcggcacc ttgggttgtg tatcgggatg ggtcatctgt tttccgaaga
      421 ttgttccaga gcgcccatgg attcgtctcg tctacgcatc tcggcgatct gatgtttcgc
      481 cgccggcacc cagagccatt gccccgggtg ttgtcgaaca cgtccttcca gaactgtggc
      541 aagagtctgc atcgctttct cagtctcaat cgaactcgcg ccctgagagg gtacgtcgag
      601 aggcttttcg aacagcagat gaatccgacc ggtctcatct cggtaactga ccgagggaat
      661 cagcgcagcg ccggttcgtt gagcgaagaa aagaggccct ttggggaaaa gatagtcgtt
      721 gccacagaaa cgcacaggaa ctccattctc ccactgccct agatcaagca cgaacgcaat
      781 ggggcgattg gctttcagat cccggtagat tcttatctgg gcagaagcat cgggaaagat
      841 ggtgacaagt cctgtttggc ttctgagatt ttcaagatgt ttttggatga atcggtttct
      901 cgctgtccag gcaaccccac gcagtggata gcctagaagt gagagtgcac tgcccaccaa
      961 ttcccagttt cccagatgaa ctgtcgccaa aactgcaccc ctgcccgcgg caagtgctgc
     1021 atcgaggcgc tcgagatgat caaaggtcac aaactctctg ggaccctcgt gaaccatcct
     1081 ctggatccag tccaactcga gaagattgcg caaaaaactc ccaacaagct tctcagtgag
     1141 acactcgcac tgaaaaacag acggaacacg cacactcgaa actctttcgg ccgatatggc
     1201 cttgtcggtt cccatgccgc ctggctcgct catcaggagt ttggcatact tatcgaggct
     1261 ctgctccctc atgccggtgc tgaatgcaca cgtcagcgtt gaaagaaacg agattgtcct
     1321 atcaacagcc catgagggga caaggcgact gtaactccag ctcagacgca gtaccgtcca
     1381 ttccaggtaa tctcgaatgc gagaaagggt ctcattcatc tccctgccca ctctttcatt
     1441 cttattgctg gtaccatagc agaaaccgaa tacaggtgca tcccaagaaa ctaatccgct
     1501 gagagacaag actgagaatt ctggccgatt ttctccaaca gtagtatcgc tgactctaac
     1561 tgcgggaatg ttgcctcaat tgcctttgat caaatctgaa cccgtgcagg ggcttatgtg
     1621 ttaatgtttt tccgtctgac tgcagcgaac cgcagaagat gccaaaggag tgaagcagaa
     1681 gatgataaga atactcccgg aattcggagt cgcctgctgc gtgatcattg tgtgtttgag
     1741 tgccagaatc tgtctggcag accaaggtga tgaactcccc aagcccgcct acatcgtact
     1801 ggatccgaat gcagctcgcc ctccgttcgc cgaaggtgag cctgttcctc aactcatgaa
     1861 gccaccccag gcaccgcgac gcaccttcgg tgtgcccacc attcgttcga ccagcacagc
     1921 tgaaggggaa cgcattgaac tggacaacga ggtcatcaat atcactctga cggtggactg
     1981 ccaccgcaga gtcctgatca gcagcattcg tactggaaac cagcgtgaga ttctgtctgc
     2041 acctgcctct ccctttgtgg tcgttgaccc ggaaggccga gtgaccagag acgcatcgga
     2101 catttccgtt aagtcgtgga tgccgagaca gcactccatc tataccgaga tcgagattcg
     2161 actcaccgca ccggaacccg tgctctggcg agtgagactg tatcggaaca aacccttcct
     2221 agaacagcag atcagcgttc cggtgcattg gcgtgatcgc ggttgtgccg tcgcgcagat
     2281 actgcccaca ggcgagatgc tgaaaccagt catgccaaac aattcgtttg gcagaggctt
     2341 ctccgagggg aaacccaatc tcaaggggat gcaccgtttt gagttcgttt cacagtccga
     2401 ccacctgtgc tatgaccccg agagcgtgag cggtgtggca gccttcgttg ccggcattgg
     2461 cggtgaggag cgtattcagc aaggtcatcc gcccct
//
LOCUS       DAOQUC010000537         2493 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_51000_length_2493_cov_2.979081, whole genome shotgun sequence.
ACCESSION   DAOQUC010000537 DAOQUC010000000
VERSION     DAOQUC010000537.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2493
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51000_length_2493_cov_2.979081"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="PKH07_09750"
     CDS             complement(<1..287)
                     /locus_tag="PKH07_09750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35268.1"
                     /translation="MLIWFGISAAFCVLFLFESVSHAHQLRKPPHTVDRLLLLQRAED
                     FAGGSFDHCQLVSESTPAVTLKAKGDGTYPERGVYTSREFMSDFPFNELLP"
     gene            complement(493..945)
                     /locus_tag="PKH07_09755"
                     /pseudo
     CDS             complement(493..945)
                     /locus_tag="PKH07_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018449919.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
     gene            complement(942..1547)
                     /locus_tag="PKH07_09760"
     CDS             complement(942..1547)
                     /locus_tag="PKH07_09760"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HNT35269.1"
                     /translation="MILLIDNYDSFTYNLAQYLGELGAELHIRRNDRITLGDIEKMAP
                     RAIVISPGPCTPLEAGISNDVILKFGQSTPILGVCLGHQCIGHVYGGVVKRARQVMHG
                     KTSMIYHTGRTIFNGLANPFEATRYHSLIVERETLPKCLQITAETDSDEIMGLKHVDF
                     PVWGVQFHPESILTREGKHLLANFLDLACSESRVPAQEVKR"
     gene            complement(1544..>2493)
                     /locus_tag="PKH07_09765"
     CDS             complement(1544..>2493)
                     /locus_tag="PKH07_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016482288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HNT35270.1"
                     /translation="PDAAYEEAIGKIDRLVEKLRKPLEDIPFGCEGADKLDVKSNMSE
                     QQYVEAVRRSKEYILAGDIFQVVLSLRQQVSIQCKPFDIYRALRTINPSPYMFYIQYG
                     DLHLAGSSPEILVKLLGDTVQVRPIAGTRARGRSLEEDARLEKELLADEKELAEHIML
                     VDLGRNDVGIVSRSGTVKVTDLKVIERYSHVMHIVSNVEGQLRPEKDAFDVLRACFPA
                     GTVSGAPKIRAMQIIEELEPTRRGPYAGALGYISVSGDLDTGITIRTVVIKGDTAYVQ
                     SGAGIVADSVPEKEYEEAQNKARGMLKAIQLAEGARGIR"
BASE COUNT          576 a          736 c          614 g          567 t
ORIGIN      
        1 cagggaagca gttcgttgaa cgggaaatca ctcataaact cccgtgaggt atagacgcct
       61 ctctcaggat atgttccgtc ccctttggct ttgagtgtaa ctgcaggagt tgactcactt
      121 accagctgac agtgatcgaa cgaaccaccc gcgaaatctt ccgctctctg aagcagcaac
      181 agacgatcta cggtgtgcgg gggctttctc aactgatggg cgtgggaaac ggattcaaaa
      241 aggaaaagga cacaaaaagc agcggaaata ccgaaccaga tcagcactcg atttttcacc
      301 tttctatgat ttcgcataca gttcatcagg atcaattcct gcgtctcgca tggcttcccg
      361 cgccgaaggg gtcagcgttg aacccttcgc cagccggatg ctctgtcccg actccaaagc
      421 cttcctgaca tcgtcggccg tcagacaggt gccctgcagt gttgttccgg acttctctga
      481 acagcagccc ccgaggggtt tgagatgccg cttttccact gccagaaact tgtccaggcg
      541 accttgatac ttcccgccga gaaactttga accgaggaac gttttcacaa tctccatggc
      601 cagtgcttcg cccaacactt ttccaccaag gcacaacaca ttggcgttgg agtgttcctg
      661 actcatacgg gcgctgaagg tgtccatgca gaccgccgca taaacgccag gcagtttgtt
      721 cgccaacatt gcggacgagt acccaacccc atccaccaga atggcacgct cacattctcc
      781 catcgctaca gccatggcag ccggatagat caaatcaggc cagtccaccg cttcttttga
      841 atgggttcca aaatccaccg gctcatgacc catctcgacc aacgccttgc ggacgatttc
      901 cttcagttcg aacccaccat gatcagcagc cagtgcaatc ttcatctttt cacttcctgt
      961 gcaggcactc tggactcgga acaggccaag tccaggaaat ttgccagcag atgtttgccc
     1021 tcacgggtca agatggattc cggatgaaac tgaacgcccc aaaccggaaa gtccacatgc
     1081 ttcaaaccca tgatctcgtc actatcggtt tccgcggtga tctggagaca ttttggaaga
     1141 gtctcccgtt ccacgatcag agagtgatat cgcgttgcct caaaaggatt tgccaacccg
     1201 ttgaagatgg ttcgccctgt gtggtagatc atggacgtct taccgtgcat gacctgtcgg
     1261 gcacgcttca cgacgccccc gtagacatga ccgatacact gatgccccag acagacaccc
     1321 agaatcggag tgctctgacc gaacttcaga atgacgtcat tggagatgcc ggcttcgagc
     1381 ggcgtacagg ggcctggaga gatgacaatc gctctgggtg ccatcttttc gatgtcgccc
     1441 aaagtgatgc gatcattgcg ccgaatgtgc aactccgcgc ctaactcccc caggtactgg
     1501 gcgaggttgt aggtaaatga atcgtagtta tcaatgagaa ggatcatcga atgcctcgcg
     1561 ctccttccgc cagctgaatg gccttcaaca tgcctctggc cttgttctga gcttcctcgt
     1621 attccttctc aggaaccgag tccgccacaa tgcccgctcc cgactgcaca tacgccgtgt
     1681 cccctttgat gacgacggtt cggatcgtga tgccggtgtc cagatcgcct gagacactga
     1741 tgtatcccaa agcacctgcg tagggacccc gacgggtcgg ttcgagttcc tcgatgatct
     1801 gcatggcccg aatcttcgga gcgccggaaa cagttcccgc cggaaaacag gctcgaagca
     1861 catcaaaagc gtccttttcc ggtcgcaatt gtccctcaac gttggagaca atgtgcatga
     1921 catggctgta acgctcgatg actttcaaat cggtcacttt gaccgttccc gaacgcgaga
     1981 cgatgccgac atcatttctg cccaagtcca ccagcatgat gtgctcggcc agttcttttt
     2041 cgtcggcaag cagttccttc tccaggcggg catcttcttc cagacttcgc ccgcgggcgc
     2101 gggtgcctgc aatgggtcga acttgaaccg tgtctcccag aagcttgacg agaatctccg
     2161 gcgaggaacc cgcgagatgc agatcgccgt attgaatata gaacatgtag ggcgaagggt
     2221 tgatcgtccg cagggcacga tagatgtcaa acggcttgca ctgaattgag acctgctgac
     2281 gcaaagacag aaccacctgg aaaatgtctc ccgcaaggat gtactccttc gaacgccgaa
     2341 ctgcctcaac atactgctgt tccgacatat tggatttgac atcaagtttg tcggcacctt
     2401 cacagccgaa gggaatatct tccaacggct ttctcagttt ctccaccagc cgatcgatct
     2461 ttccgatagc ctcttcatag gccgcgtccg gat
//
LOCUS       DAOQUC010000538         2489 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_51136_length_2489_cov_3.721446, whole genome shotgun sequence.
ACCESSION   DAOQUC010000538 DAOQUC010000000
VERSION     DAOQUC010000538.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2489
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51136_length_2489_cov_3.721446"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            95..1219
                     /locus_tag="PKH07_09770"
     CDS             95..1219
                     /locus_tag="PKH07_09770"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HNT35271.1"
                     /translation="MSNEVTVFDLDRLINAFVECIRGNFKDDVALVLGYGSRFRGDHH
                     PKSDLDLVFINDTPRGEEASAQFVIDDIGSDFFPISWERAEGIVSFREALLSLILESA
                     VLYSRSDADLERLESLREKARWMMSPEGRPRMLRTASEHFEKCCVSLYNMRIAAERDD
                     LASLKIEAANLIHGLLFVIFMINQTYARKSPVKEVIALPIKPEGLDELIGLLMNELDP
                     QGVLAAGERLADGTRNLLVEEIRKIARPKEYPDVYTGLYEEFTGYVQKVFRACDISDP
                     HEAFFKCQLLQSEIARFLDDIESSTGWNRLLLHSDIRQAYQNANLPDLMDAATRSDFH
                     AIREAVGWLDGNLKQLFESRGVPLNVFRSIEEFESFLRNR"
     gene            complement(1736..1812)
                     /locus_tag="PKH07_09775"
     tRNA            complement(1736..1812)
                     /locus_tag="PKH07_09775"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1776..1778),aa:Arg,seq:cct)
     gene            complement(1817..2329)
                     /locus_tag="PKH07_09780"
     CDS             complement(1817..2329)
                     /locus_tag="PKH07_09780"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015144273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HNT35272.1"
                     /translation="MDLKSLIRDIPDFPKKGIIFKDITPILGNKDAFRQVIDTFADRY
                     KDKGVEYIVGVEARGFIFASALALRLNVGFVPVRKPKKLPWATHRVEYELEYGTDALE
                     IHQDAVPKGSKVVLLDDLLATGGTTAATLKLLQKLDAKVIETAFVIELGFLKGREKLK
                     DCPVYAMIRL"
BASE COUNT          590 a          627 c          662 g          610 t
ORIGIN      
        1 acgaaaggaa tcgccaagta tgagtccacg cttcggcgtg ctcatgaaga ccgcctgaag
       61 gcggaactcc tacatgagag aaaggacaat acctatgagt aacgaagtta cggtttttga
      121 cttagacaga ttgataaatg ctttcgtgga gtgtatccgc ggcaacttca aggatgatgt
      181 cgcactggtg ttgggctacg gctcacgctt ccgaggcgac catcatccga agtccgacct
      241 agacctggtt ttcatcaacg atacgcctag gggtgaggag gcttctgccc agtttgtcat
      301 cgacgatatc gggtccgact tcttccccat tagttgggaa agggcggaag gcatcgtctc
      361 cttcagggag gcgcttctgt cgcttattct cgaatcggcc gtcctctact cgcgctcgga
      421 tgccgatctg gagaggcttg aatcgctgag agagaaagcc cggtggatga tgtcgccgga
      481 gggccgaccg aggatgctga gaacggcgtc cgagcacttt gagaagtgct gcgtgtcgct
      541 gtacaacatg aggatagccg ctgaacgaga tgatcttgcg agcctcaaga tagaggcggc
      601 caacctgatc cacggtctgc tgtttgtcat ctttatgatc aaccagacct atgccaggaa
      661 atctcccgtg aaggaggtca tcgcgctgcc gatcaagcct gaaggacttg atgaactcat
      721 cgggttgctt atgaacgagc ttgacccgca aggagttctg gcagctggtg agcgtctggc
      781 cgatgggaca agaaatctct tagtcgagga gataagaaag atagcccgac cgaaggagta
      841 tcccgacgta tatacgggcc tctacgagga attcacaggc tatgtgcaga aagtctttcg
      901 cgcctgtgat atttcagatc ctcatgaggc tttcttcaaa tgtcagctgt tgcagagcga
      961 aatcgcgcgg ttcctggatg atatcgaatc cagcacagga tggaaccgtc tgcttctcca
     1021 ctcggacata cggcaagcct atcagaacgc aaatctgccc gatctgatgg atgccgcgac
     1081 gcgctctgac tttcacgcga ttcgggaggc ggtcggctgg ctcgacggga acctgaagca
     1141 acttttcgag tccaggggag tgcctttgaa tgtgttccgg agcatagagg aattcgagtc
     1201 gttcctgaga aacaggtgag ggtaacagag ggtgacccat gacgatattg cgtcatcgag
     1261 attgagagga tgagggctgt actaaatatc tcggttttat agtgcctgag ctggaagtcc
     1321 cttgctatag gaaaggcgag gaccgatttg caccgagcct cgcctcattt gctcatgtga
     1381 cagtatggac tcgtgattcc atatgcctgt cgctcagtct cctccgattg ggtcggtctg
     1441 ccggcaaata tcctgccatc tgagagcacg ggcatataga ttcctagagc aacgaccttc
     1501 gccccacctt cctcaacgac cttgtcgaat tcaatctgaa gagcaggata gtttgattgt
     1561 cgatcacgtg ttgatcaagg aagtctggat gaggcaattt ctggagattc tcaccccaag
     1621 accgagtacg ctccgaacgt gtcccagagg gcattttctt ttttgagatt gtcgcaaaat
     1681 tgtgacattt caaaagtgaa ggggttacgc cgttgacgta accccttgat tttgctggta
     1741 cgcccggaag gattcgaacc ttcggcctac ggtttaggaa accgccgctc tatcctactg
     1801 agctacgggc gcagaatcag agtcggatca tggcataaac agggcagtct ttcaacttct
     1861 ctcttccctt cagaaagccc agttcaatga cgaatgccgt ctcgatgact tttgcatcca
     1921 acttctgcag cagtttcaat gtcgccgccg tcgttccacc ggtggccaga aggtcatcca
     1981 gaagcaccac tttgctcccc ttcggcacag catcctgatg aatctccagt gcgtcggtgc
     2041 cgtactccaa ttcgtattca acccgatgcg tcgcccaagg gagctttttc ggtttgcgaa
     2101 caggcacaaa gcccacgttg agccgcaaag ccaaggccga agcgaagata aagccacggg
     2161 cttcaactcc gacaatgtac tcgacgccct tgtctttgta gcggtctgca aaggtgtcaa
     2221 tgacctgccg aaaggcatct ttgttaccaa gaatcggtgt gatgtccttg aagataatcc
     2281 ctttctttgg aaagtcggga atatctcgga tcagtgactt caaatccatt tctgttttgt
     2341 cctccaagtt gaactcattt attatctgta acaacagcgc attattgcgc gtccaaaaag
     2401 caaggtattc cggtcgtcct aatccccctt attcccctta ataggagggg ttggagggtt
     2461 cttcagactt cctattaagg ggaataagg
//
LOCUS       DAOQUC010000539         2484 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_51290_length_2484_cov_3.605187, whole genome shotgun sequence.
ACCESSION   DAOQUC010000539 DAOQUC010000000
VERSION     DAOQUC010000539.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2484
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51290_length_2484_cov_3.605187"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            99..>2484
                     /locus_tag="PKH07_09785"
     CDS             99..>2484
                     /locus_tag="PKH07_09785"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNT35273.1"
                     /translation="MGESVLALLALPDANSDGWPEVVAATDKGVVTAFQAGGANAGAI
                     LWTQTSTCGVNVLSVFQDRNADGYPEVLFAGADQRVHLVSGKTGVHLWNRYLSASGYA
                     YVHVVAASQDLNGDTVFDPVAWTWDGRLWALNGTNGTDLWRVSIDGGFTEGMTCAGDI
                     NGDGTPDFLAGGNSMSLKLCSGTNGSVLWTCPLGRPIRDVVVVDDVTGDGVKDGFACT
                     AGGVIACISGAGSGTVTPVWTASIGDVCRMLARPTDCNGDGKPDAVVCGENGEVACFS
                     GSDGAELWRWQGPDVVRAIELVADVDGDAIADIAAACLDGTIALLPNAPSAWGVPKAD
                     PSAGKVLEFIPADSSIRMSRSDIPSAVPLEHPGLEPFATEVPSKNLAIAAGATSVPIL
                     LYHDVIPEAFYPYGVSLDNFRAQMDLVVQGNYTCVSLDLIADWIAGTAELPENPICIT
                     FDGPYEGHHTWAYPILQERGLSAVSYITSDWIGTANHADWHQLREMDAAGIEDVQNHT
                     INHPSLTSLTQEAAIAQLQGCSASLRIHMGKESFHHAYPGGSYNSSVMQILRTLGFKT
                     ATTVVQRHVVRTDDPMALPRYSVLVNTTIAEFKTKIRYAEITPTPLPEKTLPYQFVSN
                     VGSGWTAPSYADVDAEGKLWVCDYNAKQVRVFSSSGVEMGFSPITQGLNQSGVVVPME
                     APSGVAVTPSGDVLISICDYFGSPQYLGLFRYRASDGQVLNGWDINYKIGDVDCDANG
                     LIYTVNKVGGDWHVYTSNMTEVAGSPFGTYVSSNISRGISVRPDSTRVYIISETD"
BASE COUNT          536 a          628 c          731 g          589 t
ORIGIN      
        1 acagcaaaga tgaaattcta gcgggtctgg attctggcca ggtcttgtgt ctgagcacgc
       61 agtcagcaac agaagcaacg gtgctttgga gcgtctcgtt gggagagtct gtgcttgcgc
      121 tgctcgcatt gcctgatgca aacagcgacg gttggccgga agtggttgcg gcaacggaca
      181 aaggcgtggt cacggcgttt caagcaggtg gtgccaacgc gggtgcaata ctctggaccc
      241 aaacatccac ctgcggtgtc aatgtgctgt cggtcttcca ggatcgtaac gccgacggat
      301 atcccgaagt gctcttcgcc ggtgcagacc agcgtgttca ccttgtttca ggcaagaccg
      361 gagttcatct ttggaacaga tatctgtcgg cgagtggcta cgcttatgtt cacgttgtgg
      421 cagcaagcca ggatctcaat ggagacacgg tcttcgatcc cgttgcctgg acatgggacg
      481 gaaggctttg ggcgctcaac ggaaccaacg gtacagatct ttggagagtc tccattgacg
      541 gtggattcac tgagggcatg acctgtgccg gagacatcaa cggggacgga acgcccgact
      601 ttctcgcggg tggcaactcg atgtccctga agctctgttc gggaacaaac ggatctgtct
      661 tgtggacatg tccgcttggg cgaccgattc gggatgtcgt ggtcgtagat gatgttacag
      721 gcgatggggt caaggatggc tttgcctgca ctgcgggggg cgttattgcc tgcatttcag
      781 gagccggttc gggaaccgtg actccggtct ggactgcctc gattggtgat gtgtgccgaa
      841 tgttggcacg tcccacagac tgcaacggtg acgggaaacc tgatgccgtt gtgtgtggtg
      901 aaaacggaga ggtagcctgt ttttcgggaa gtgatggtgc agaactctgg cgctggcaag
      961 gaccggacgt cgttcgtgcc atcgagttgg tcgcagacgt tgatggagat gcgattgccg
     1021 atatcgccgc ggcttgtctg gatggaacga tcgcactgct tccaaatgcc ccgagtgctt
     1081 ggggtgttcc caaagcagat ccgagtgcgg gcaaggtgtt ggagttcatt cctgcagaca
     1141 gttcgatcag gatgagtcga agcgacatcc caagcgcggt gccgttggag catcctggcc
     1201 tggaaccttt tgcgacggaa gtgccatcaa agaatctggc gatcgcggct ggtgcgacca
     1261 gtgttcctat cttgctctat cacgatgtca tccctgaagc gttctatccc tatggtgtat
     1321 cgctcgacaa ctttcgggca cagatggatt tggtggtgca gggcaactac acatgcgttt
     1381 ccctcgatct gatcgcagac tggattgccg gtacggcaga actgcccgag aatccgatct
     1441 gcatcacatt cgatggcccc tacgaaggtc atcacacctg ggcatatccg atccttcagg
     1501 aacgagggct ttctgctgtt tcgtacatca cgtccgactg gattggcacg gcaaatcacg
     1561 ccgactggca ccaactgcgc gagatggatg cagcgggtat tgaggatgta cagaatcaca
     1621 cgatcaacca tccttctttg acttctctca ctcaggaggc cgccattgcc cagctgcagg
     1681 gctgcagtgc ttcattgagg attcacatgg ggaaagagtc ttttcaccat gcgtatcccg
     1741 gagggtctta caattcttct gtcatgcaaa tcctgagaac cctcggcttc aagacggcaa
     1801 caaccgtcgt gcaacgccat gttgtcagaa cagatgaccc aatggcgctg ccgagatact
     1861 ccgttttggt gaatacaacg attgcagaat tcaagacaaa gattcgttat gcggagataa
     1921 caccaacgcc gctgcctgag aaaacacttc cctaccagtt tgtttcgaat gtgggtagtg
     1981 ggtggacagc gccgtcctat gccgacgtgg acgccgaggg aaaactctgg gtctgtgact
     2041 acaatgcaaa gcaagtgcgt gttttcagct cgtccggtgt agaaatgggc ttttccccca
     2101 tcacacaggg tctcaatcag tcaggtgtcg tggtacccat ggaagcgccc tcaggagtgg
     2161 cggtcactcc ttctggtgat gttctcatca gcatctgtga ctattttggc agccctcaat
     2221 atctggggct gtttcgatat cgcgcatcag atgggcaagt tctgaatggg tgggatatca
     2281 actacaagat cggagatgtg gactgcgatg ccaacggcct gatatatact gtcaacaaag
     2341 tgggtggcga ctggcatgtg tacacgtcaa atatgacaga ggtcgctgga agcccgtttg
     2401 ggacttatgt gtcgtcaaac atttcccgtg gcatcagcgt ccgtcctgac tccacccgcg
     2461 tctacatcat ctcagagaca gatt
//
LOCUS       DAOQUC010000540         2480 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_51419_length_2480_cov_3.044124, whole genome shotgun sequence.
ACCESSION   DAOQUC010000540 DAOQUC010000000
VERSION     DAOQUC010000540.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2480
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51419_length_2480_cov_3.044124"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..173)
                     /locus_tag="PKH07_09790"
     CDS             complement(<1..173)
                     /locus_tag="PKH07_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015574701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HNT35274.1"
                     /translation="MDRFQPIGLFDSGIGGLTVAREIKALLPEERLVYFGDTARVPYG
                     SKSRNTIQIYTRE"
     gene            complement(180..929)
                     /locus_tag="PKH07_09795"
     CDS             complement(180..929)
                     /locus_tag="PKH07_09795"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNT35275.1"
                     /translation="MIFAVLPAYNESLALPLLLEEYCELSPQLSEPLRVVVVDDGSTD
                     STVQTAQTFSERLELVILEHKVNRGLGAAFLTGFSYVAQAGKTSDSVVTMDADNTHSP
                     RYIPEMLASLNNSDIVIASRYASGGKETGVPFLRSLLSHGASLLYSRLIGIPQVRDYT
                     CGYRVYRLGIIQQGIEKYGSEFIQEPGFCSTGEILVKLAPLTESISEVGFELRYDLKG
                     GMSKMPKVKTVLRTLQLLRKLRRISRTLKVE"
     gene            complement(1079..>2480)
                     /locus_tag="PKH07_09800"
     CDS             complement(1079..>2480)
                     /locus_tag="PKH07_09800"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNT35276.1"
                     /translation="FFCLSVFLGTLGLIFARQYEQAKVDLSSLEKVLIAGRAFWFYIG
                     KTLWPGNLMAVFPKWDISPTSPWQYLFPLGASVLLVTLWIASRRVGRGPAVAVSFFAI
                     TLAPTLGFISFPLFDYTQVALRYTYLASVGLSALLAVLASRCAERFRLSPRTKQSVAA
                     GVLTLLGILTFQQARTYHDMETLFRHCLNKNPKALLAYINVGQTLQEQGKTNEAVSFY
                     EKALAIDPNLPSAHYNYAICLEQLGKDDEAIGHYERALEHMPKSPSRADIHNNLGKIL
                     VRHGPAERAEEQFRAAIAVNPKHALAYSNLARFLVGQGKMDEARESALQAIRIAPQFP
                     AAHYNLALTYSRSGRPELAIEHFRTALRFDPEFSQAALELAWILATSPNDTLRNGIVA
                     SEMAERVCSKSDGATATALDVLAAAYAETGQFAQAVRTAERALARAQAENKQHLVEPL
                     RARIDLYRSNRPYRGK"
BASE COUNT          588 a          654 c          664 g          574 t
ORIGIN      
        1 ttttcacgag tatagatttg aatcgtgtta cgtgattttg agccataggg cacccgcgcg
       61 gtatcaccaa agtaaaccag acgttcctca gggagcagag ccttgatctc cctggccacg
      121 gtcagaccac ctatacctga gtcaaacaga cctatgggtt ggaatcgatc catcatgggt
      181 cactcaacct tcagtgttct gctgatcctt cgcagtttcc ggagcaattg aagagttctc
      241 agcaccgtct tgactttggg catcttactc atgcccccct tgagatcata gcgcagctcg
      301 aaacccactt ccgatatgga ctcggtgagt ggggcgagtt tgacaaggat ctcgcccgtt
      361 gagcagaacc caggctcctg gatgaactct gatccatact tctcaatgcc ttgctggatg
      421 atcccgagtc ggtagacacg atacccacat gtgtagtctc tgacttgtgg aatgccaata
      481 agcctgctgt acagcaggct tgctccgtga ctcaacagag aacgcaggaa aggaacaccg
      541 gtctcttttc cgcccgaagc ataccttgag gcgatcacga tatctgagtt gttcagagag
      601 gcaagcatct caggaatgta gcgcggtgaa tgagtgttgt ccgcatccat cgtcacaacg
      661 ctgtcactgg ttttgcctgc ctgtgcgaca tacgaaaaac ccgtcagaaa ggcagccccg
      721 agtcctcggt taaccttatg ctccagtatc acgagttcaa gtctttccga gaacgtctga
      781 gcggtttgca ccgtggagtc tgtcgaaccg tcgtctacaa cgaccacccg cagaggttcc
      841 gatagctggg ggctcagttc acagtactcc tcaagcagca gaggcagcgc caaggattca
      901 ttgtatgcgg gcaggacagc aaagatcaca gaagaacatc catatcccag aacgttttga
      961 gcagcacaaa caaccgctct atactaatat gggacaaatg cctaccatgc cgcccgcatt
     1021 ctgtaaacca aaatccgtta cggcgatcga aatgtcatgt tcacaggcac cgtagcggct
     1081 atttccctcg atagggtctg tttgacctgt acagatcaat tcgggctctt agcggttcaa
     1141 ccaagtgttg tttgttctcc gcctgggctc gcgcaagagc ccgctctgca gtgcgaactg
     1201 cctgagcgaa ctggccggtt tctgcatagg cggctgccaa gacatccagc gctgtggctg
     1261 ttgccccgtc ggatttgctg cagactcgct ccgccatctc tgaggcgacg atgccattgc
     1321 gcaaggtgtc gtttggggat gtcgcaagaa tccaagccaa ttccagagcg gcttgtgaaa
     1381 actctggatc aaaacgaagt gccgttcgga agtgttcgat cgctagttct ggccgcccgg
     1441 aacggctgta tgtcagtgca agattgtagt gggctgctgg gaattgaggg gcgattcgaa
     1501 tcgcctgcag agcgctttcg cgcgcttcat ccatctttcc ctgcccgaca agaaagcgcg
     1561 caagattact gtaggcgagg gcatgcttcg gattcacggc aatggctgca cggaactgtt
     1621 cttctgctct ttcggccgga ccgtgcctga caagaatctt ccccagattg ttgtgtatgt
     1681 ctgctcttga cggagacttg ggcatgtgtt ccaaggctct ttcatagtga ccaattgctt
     1741 catcatcctt gcccagttgc tcaagacaga tggcatagtt gtaatgtgca gagggaaggt
     1801 tgggatcgat cgccaaggct ttctcataga acgagacggc ctcgttagtt ttcccctgct
     1861 cctgcagtgt ttgaccgaca ttgatgtacg ccaataacgc cttggggttc ttgttgaggc
     1921 agtgtcgaaa gagcgtctcc atgtcatgat atgttcgcgc ctgttgaaac gtcagaatgc
     1981 ccagaagtgt aaggactccc gctgcgacac tctgcttggt tctgggtgac agcctgaacc
     2041 tctccgcaca ccgtgaggcc aggacagcga gcagggcaga gagtcccaca cttgcaagat
     2101 atgtgtagcg aagagcgact tgcgtgtagt caaaaagagg aaaactgatg aaaccgagtg
     2161 tgggagcaag cgttatcgcg aaaaacgata ccgcgacggc ggggcctctc cccacgcgcc
     2221 gagaggcaat ccaaagggtc acgagcagta ctgaggctcc gagaggaaag agatattgcc
     2281 atggagaagt tggactaatg tcccattttg gaaaaaccgc catcagattg ccaggccaga
     2341 gcgtctttcc gatatagaac caaaaagcgc gaccggcaat cagcactttc tcgagtgacg
     2401 acaggtctac cttggcttgt tcatactgtc tggcgaagat tagtccaagg gttcccagga
     2461 acaccgagag gcagaagaag
//
LOCUS       DAOQUC010000541         2478 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_51486_length_2478_cov_1.951713, whole genome shotgun sequence.
ACCESSION   DAOQUC010000541 DAOQUC010000000
VERSION     DAOQUC010000541.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2478
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51486_length_2478_cov_1.951713"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1225
                     /locus_tag="PKH07_09805"
     CDS             <1..1225
                     /locus_tag="PKH07_09805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35277.1"
                     /translation="WPGYENLKPFMNGILADIQAPARLIHAWESTFWWGGTRVLPEGP
                     VQYPGDRRAFATARNDIRNVWRNLSDNPPKYDQFVDVGMAAWVESDPYNLYIGWPSGY
                     LTDLPWSNLPYALAYSDEYVWVFDTHTQYPQTKDVLNPFMASIANRTFNTGEERVSSV
                     SEDFQTDPMKRGWYFDFDMLDIGQEVHEGFLPAMNTDNVAYVWSESAEAVQVKGAWLG
                     GLHGDTVVLMDKQRRRYVRPLQPFGRNSHFEAEMDFYITDFGTDADNPIVLGLFNSDK
                     RVDQQSLILRIAGSNSATVTIAGDGTRWDVPLSLSSALQTSRTYRIQFDYDGTSGRFK
                     ATLTDVAGSSVIAQLQQTPPSSIGLFELDEIGAAMWDAIATMTTPEQAYKYFLQSVSL
                     TTILPSSVENWMMMK"
     gene            1290..>2478
                     /locus_tag="PKH07_09810"
     CDS             1290..>2478
                     /locus_tag="PKH07_09810"
                     /inference="COORDINATES: protein motif:HMM:NF027224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BNR-4 repeat-containing protein"
                     /protein_id="HNT35278.1"
                     /translation="MVFFGIVAFLALSGNFVNTSSHPIEISMTLIDDHAIGYATFQSH
                     NQKVVSNQHGIFLTYLKTRTDAYDSQLWRLMRSTDGGTTFSLVYEATAATHPPAIETD
                     GDGNIYLMHGNISNGQGRLYRFLAKEDFKIPNYTVIPNSWADKFSTFLDMERKQIYFA
                     QVSHSDDETFYAIGLDGAVNFTQRLTVHGPHAYFLYPHLAMSEEGILHLAYTTFKNGN
                     GYMYWDIHHILSDDAGQTWKTLAGANVTIPFIPDDTGPIPRITKDNEFDVQTWLSNMA
                     VKKGKVHFLYEAQFNPCQQNYVRYDIESATEDIRVTPVFKGESIRLGHLDGFFAHDAH
                     SPDGPLFCIARYIPQDVRRIGCLVSRDNGVTWHDHALSAPVVNPYSIGGCRQLTSDGY
                     VIGS"
BASE COUNT          624 a          633 c          622 g          599 t
ORIGIN      
        1 atggccagga tatgaaaact tgaaaccttt tatgaacggc attctagccg acatacaagc
       61 gccggctcgc ctgattcatg catgggaatc aaccttctgg tggggaggaa cgagagtgct
      121 tcctgaggga ccagttcaat accctggtga ccgtcgtgcc tttgccacag cacgcaatga
      181 catcagaaat gtctggcgta acttgagcga caatcctccc aagtatgacc agtttgtgga
      241 cgtgggcatg gcagcgtggg tggaaagcga tccctataat ctgtacattg gctggccaag
      301 tggttatctg acagatctgc cctggtcgaa tctcccgtat gcgcttgcct acagcgatga
      361 atacgtctgg gtttttgaca cacacaccca gtatcctcaa accaaggacg tgctgaatcc
      421 atttatggcc tccattgcca accgcacctt caataccggg gaagagcggg tcagcagcgt
      481 ctccgaggat ttccagaccg atccgatgaa gcgtggctgg tactttgact tcgacatgct
      541 tgatattgga caggaggtgc atgaaggttt ccttcccgcc atgaacacgg ataacgttgc
      601 ttacgtatgg tctgagtccg ctgaagctgt tcaagtcaag ggagcatggc tgggaggact
      661 tcatggcgac accgttgtct tgatggataa acagcgccga cgctatgtgc gtccgctaca
      721 gccttttggc aggaatagcc attttgaagc tgaaatggat ttctacatca ccgattttgg
      781 gactgatgcg gacaatccca ttgttctggg attgttcaac agtgacaaac gagttgacca
      841 gcaatccctc attctgcgca ttgccggttc aaactccgca acagtaacga ttgccggaga
      901 cggaacgcgg tgggacgttc ctctctctct cagcagtgcc ctgcaaacat cccgaacgta
      961 caggattcag ttcgactacg acgggacctc tggtcgtttc aaggcaaccc tgacggacgt
     1021 ggcgggttct tccgtcatcg cgcagttgca gcaaacacct ccttcgagca ttggtctttt
     1081 tgaactggac gaaatcggtg cagcaatgtg ggatgctatc gcaaccatga ctacgcctga
     1141 gcaagcttac aaatacttcc ttcagagtgt ctcgttgacg acgattcttc catccagcgt
     1201 cgagaattgg atgatgatga agtagattct gacggataga agatatcgca gaatcagaat
     1261 gtcttggcac ctcaaggaca tatagtgaaa tggtcttctt tggaatagtg gcatttttgg
     1321 cgctttcggg gaactttgta aacacgagtt cgcacccgat cgagatcagc atgacgttga
     1381 ttgacgacca tgccatcggt tacgcgacgt ttcagagcca caatcagaag gttgtcagca
     1441 accaacatgg gatctttctt acttatttga agacccgcac tgatgcgtac gattcacagc
     1501 tctggcggtt gatgcgaagc accgacgggg gaacgacgtt cagccttgtc tatgaggcaa
     1561 cagccgcaac gcatccgcca gcaattgaga ccgatgggga cgggaacatc tatcttatgc
     1621 acggaaacat aagtaacggg caaggtcgtc tgtatcgttt cctggcaaaa gaggatttca
     1681 agattcccaa ttacacagtc attccaaatt cctgggctga caaattcagc acgttcctgg
     1741 atatggagcg aaagcaaata tacttcgccc aggtctccca ctccgatgac gagactttct
     1801 atgccatcgg actggatggc gcggtaaatt tcacccaacg gcttacagtt cacggtccac
     1861 acgcctattt cctgtaccca catctcgcaa tgtcggaaga aggcatactc caccttgcct
     1921 acaccacatt caagaatggt aatggataca tgtattggga cattcaccac atactgtcag
     1981 atgacgctgg acaaacatgg aagacgctgg cgggagcaaa cgtgactatt cctttcattc
     2041 ccgatgacac gggtcccata cctcgcataa caaaagacaa cgaatttgat gttcaaacat
     2101 ggctctccaa catggcggta aagaagggca aggttcattt tctgtacgaa gctcagttca
     2161 atccctgcca acagaattac gttcgatatg acatagaatc tgcaactgag gatattcgtg
     2221 tgacccctgt tttcaaggga gaatctattc gactggggca tcttgacggt tttttcgccc
     2281 atgacgcgca cagtccggac ggacccctgt tctgcatcgc cagatacatt ccccaagacg
     2341 tcagaagaat cggatgtctg gtgagtcggg acaatggagt tacgtggcac gatcacgccc
     2401 tcagcgcgcc tgttgtgaat ccttactcca ttggcggatg ccgccagttg acctctgacg
     2461 gatacgtcat cggcagtt
//
LOCUS       DAOQUC010000542         2476 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_51543_length_2476_cov_2.574143, whole genome shotgun sequence.
ACCESSION   DAOQUC010000542 DAOQUC010000000
VERSION     DAOQUC010000542.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2476
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51543_length_2476_cov_2.574143"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..115)
                     /locus_tag="PKH07_09815"
                     /pseudo
     CDS             complement(1..115)
                     /locus_tag="PKH07_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007293152.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(116..1387)
                     /locus_tag="PKH07_09820"
     CDS             complement(116..1387)
                     /locus_tag="PKH07_09820"
                     /inference="COORDINATES: protein motif:HMM:NF014135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HNT35279.1"
                     /translation="MLEFSMVSLVVFIAAYVLFIFLPHKRTLVSVAAAFVIVLSGCIS
                     LREAVAAINWNVMGIFVGTLVLADIFMESRVPAYLAEIIVDKAKSTCWAILFICMMTG
                     FISAFVENVASVLIVAPIAMALAQKLKINPVNMMLGIAVSSNLQGAATLIGDPPSMLL
                     GGYARMTFMDFFFFKGKPSIFFGVELGALMSVFVLYFFFRQHREKTESVAIEKVQSWF
                     PTILLVSLIIMLALSSFFDKGFSYLAGIICMGFGAVATLWEKLINKGSIVEGLKKLDW
                     DTTAFLMAVFILVGSLTITGWIDRIALSLSNIFGGNTFMGYTAIVFISILVSAFVDNV
                     PYLAAMLPVAVSLSAKMGIDPTLFLFGLLIGASIGGNITPIGASANIVACSILKKEGY
                     HVSFWEFAKIGLPFTLTAVIPAYLFFWFIWN"
     gene            complement(1486..>2476)
                     /locus_tag="PKH07_09825"
     CDS             complement(1486..>2476)
                     /locus_tag="PKH07_09825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35280.1"
                     /translation="EVSEQTWRTSCWAYYIPGMTATEFGFKDQGGNTEASVPINTITW
                     GFYSVDWLIADNIDSQAWGTGTQGVVIDDVRAGPASKMNWITDWIWNGTHDSGSKSTR
                     LTTDYFAADGGEASILPAPGSVNGGRMWTATSRGDGFINLNETIGGSPTNCAAYAHIY
                     VIADTAKSGVRLLAGSDDGVRVFLNGALIHNNDIDRSHDYFNPDTDAISGLSLNAGQN
                     RLLVKVRQETGGFSLSARFCDINGNALSGLTYSLSPILVPTNTPTFTATWTPTHTPTW
                     TSTQTHTPTITPSWTPSWTPTRTPSWTPTNTAIPTPTPVVTHNTAVENWMMLR"
BASE COUNT          645 a          627 c          626 g          578 t
ORIGIN      
        1 cgctttcgca ccattcgctc atcaggaaaa gacaaatcag acagtcttcg ttagtgaaaa
       61 acaccctctg atagcgattg ccacgctgtg ttacatggtg agggtatccc ggcgcctagt
      121 tccagataaa ccagaaaaac aggtatgcgg ggataacagc cgtcagcgta aaaggcagac
      181 cgattttcgc aaattcccag aacgagacat gataaccttc ttttttcaaa atggagcatg
      241 cgacgatatt tgcggaagcg cctatcggtg ttatgttgcc tccgatgctt gcgccgatca
      301 ggagtccgaa cagaaacaag gtcggatcaa ttcccatttt agccgacagg gagaccgcga
      361 cgggcagcat agctgcaagg taaggcacat tgtccacaaa cgcggacacc agaatggaaa
      421 tgaaaacgat agcggtgtac cccatgaagg tattcccacc gaatatgttc gagagggata
      481 acgcaattcg atctatccag ccggttatcg ttaggcttcc aacaagaatg aaaacggcca
      541 tcagaaaggc ggtggtgtcc caatcaagtt tcttcaggcc ttcgacgatg gagcctttgt
      601 tgatgagttt ctcccatagc gtcgctacag cgccgaaacc catgcatatg atccccgcca
      661 gataggaaaa tcccttgtcg aaaaacgaag agagtgcaag catgattatc agcgatacga
      721 gcaggatggt tggaaaccac gattggactt tttctatcgc gaccgactcg gtcttttcgc
      781 gatgttgtct gaagaaaaaa tagaggacga ataccgacat aagcgcgccc aactcaacgc
      841 cgaaaaagat gctcggtttc cctttgaaaa agaagaagtc cataaacgtc attctggcgt
      901 atccgcccag aagcatgctt ggaggatctc ctatcagagt ggcggcgccc tgaagattgc
      961 ttgataccgc aattccaagc atcatattca cgggattgat tttcagtttc tgcgcaagcg
     1021 ccatggctat gggagccaca atcaaaaccg acgccacgtt ctcaacaaaa gccgatataa
     1081 agcccgtcat catacagatg aaaagaatcg cccagcatgt gcttttcgct ttgtccacta
     1141 tgatctcggc cagataagca gggacgcggc tttccatgaa aatgtctgca agaacgagcg
     1201 ttccgacgaa tatgcccatc acgttccagt tgattgcagc cactgcttct ctgagcgata
     1261 tgcagccgct caacacaatg acaaacgcgg cggcgacgga gacaagcgtt cttttgtggg
     1321 gaaggaaaat gaacagaacg tacgcggcga taaacacaac gagagaaacc atcgagaatt
     1381 ccaacattca acgctccata ttcattttgc ccgccaagaa gagcggatcg ttttggaata
     1441 taaaccaaat gaatcttcat aacaaacggc gcacgaatcc ccgactcagc gaagcatcat
     1501 ccagttctcc actgctgtgt tgtgggttac cacaggcgtt ggggtcggta tggctgtgtt
     1561 cgttggagtc catgacggag ttctcgtcgg tgtccatgat ggagtccaac tgggtgtaat
     1621 cgtgggggta tgagtctgtg ttgatgtcca cgttggcgta tgagtgggag tccaagttgc
     1681 cgtgaaggtc ggtgtgtttg tcggaacgag aattggactg aggctgtagg tcaagccaga
     1741 aagagcgttg ccgtttatgt cgcagaagcg ggcagaaaga ctgaatccgc ctgtctcctg
     1801 acggactttg accagcagtc ggttttgccc cgcattgaga gagagtcccg agatggcgtc
     1861 cgtatctggg ttgaaatagt catggcttct gtcaatgtcg ttgttgtgaa tcaatgctcc
     1921 attcaagaag acacgcaccc cgtcgtcgct tcccgcaaga agccgcactc ccgactttgc
     1981 agtatcggcg atcacgtaga tatgagcgta ggcagcgcag tttgtcggac tgccaccaat
     2041 ggtttcattg aggttgatga aaccatcgcc tcgacttgtc gccgtccaca tccttccgcc
     2101 gttgactgaa ccgggtgcgg gcaggatgct tgcttcacca ccatcagcgg cgaaataatc
     2161 ggttgtcagc ctcgtgctct tggaaccgct gtcatgcgtt ccattccaga tccaatcggt
     2221 aatccagttc atctttgagg caggacctgc acgcacatca tcaatgacta caccttgcgt
     2281 tccagttccc catgcctggc tgtcaatatt gtcagcaatc agccagtcta ctgaatagaa
     2341 tccccatgtt attgtgttga taggcactga ggcctcggta ttgccccctt gatctttgaa
     2401 gccaaactca gtggcagtca ttccaggaat gtagtaagcc caacaactgg ttcgccacgt
     2461 ctgttcgctg acctcg
//
LOCUS       DAOQUC010000543         2473 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_51611_length_2473_cov_2.997932, whole genome shotgun sequence.
ACCESSION   DAOQUC010000543 DAOQUC010000000
VERSION     DAOQUC010000543.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2473)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2473)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2473
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51611_length_2473_cov_2.997932"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="PKH07_09830"
     CDS             complement(<1..295)
                     /locus_tag="PKH07_09830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35281.1"
                     /translation="MRKSLFLSLMLVVATGAYAANPVTVDNTNTSPDGVSTFNTITSA
                     IASWCAGAANAGETAPFVINVVNGPYTENIRINKDLASLGDIVGELIIQAENPV"
     gene            complement(469..1944)
                     /locus_tag="PKH07_09835"
     CDS             complement(469..1944)
                     /locus_tag="PKH07_09835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35282.1"
                     /translation="MKKYFFLSLMLVLATGAYAGNPVTVDSTNATPDGVNTFSTITSA
                     ISSWCAGGTNAGQTAPFVINVVNGPYSENIRIDKGLVGLGDIVGELIIQAATPVNIRL
                     NPSSTVGGMLLYQDTYDVTFNNFIFSPDAGSSYVMNTLIMINDPASPAMNRNWVKFYN
                     CVFTEAYDTGAPMVTDKASALANKDALPGTRSGKMSASAGKCLMKGATNNGLQSVRLD
                     NCVLYGPPQIGFELNVRAGDDIIVHDTLAVYCAYSAYRCLGNAVANQSFTGTDAGAGP
                     TQCTAIINSPWSVASTAANGKALRFEASAAVTSTVSNVLISNSFLSGNAYVGGINING
                     NQGSVNMDNVIIDTHGDSILMQPANACTLSNATLNSSARAGIAFTAAAAGSLTVTNTI
                     FSGAGTKITGTVPTAGLSVNYSAFVESGPDAITARYAGQVITYGVNIINNDPEFKSKD
                     VTNEDFFDLASGYYPTKGIGGTDVAGGADCDATAIADWMLY"
BASE COUNT          634 a          650 c          575 g          614 t
ORIGIN      
        1 tcaccgggtt ctcagcctga atgatcagtt ctcccacaat atcacccaag cttgcaagat
       61 ccttgttgat tcggatgttc tccgtgtacg gaccattgac aacattgatc acaaatggcg
      121 ctgtctcacc cgcatttgca gcaccggcac accaactcgc aattgccgat gtgatcgtgt
      181 tgaatgtgct tacaccatcg ggactggtat ttgtgttgtc cacagtgaca ggatttgccg
      241 catacgcgcc tgtcgccaca accagcatca gactcaaaaa caaactcttt ctcatagtct
      301 acactcctta aaaactctgt tttgttgact ccaagccgaa gggaaaaggc tctgaagaac
      361 actattccct tcggtctcgg agttcattgt tcttgtctct cctgcacctg tcgctcagaa
      421 cacaacctcg gggtcagtct gtcgacagat gcttgtgtgc ttttcagatt agtaaagcat
      481 ccagtcggcg atagccgttg catcacagtc cgcaccaccc gccacgtctg tgccaccaat
      541 tcccttcgtg ggatagtatc ctgaagcaag gtcaaagaag tcctcattgg taacgtcttt
      601 gctcttgaat tcgggatcgt tgttgatgat gtttacaccg taggtaatca cttgtcccgc
      661 ataacgcgcc gttatggcat cgggaccact ctcgacaaac gccgagtagt tgacgctcag
      721 accagcagtg ggaacggtgc cagtgatctt ggtgccagcg ccgctgaaga tggtgtttgt
      781 gacagtcaga ctgccagcgg cagctgctgt gaaggcaata ccagcacgag ccgatgagtt
      841 cagcgtggcg ttgctcaagg tgcaggcatt cgccggctgc atcagtatgg aatcaccatg
      901 tgtgtcaatg attacattgt ccatgttgac actgccttgg tttccattga tgtttattcc
      961 accaacgtat gcattgccag aaagaaagct gttcgatatc agcacattgc tcacggtgga
     1021 agtcaccgca gcactagcct caaacctgag agccttgcca ttcgcagcag tagatgcgac
     1081 cgaccacggc gagttgataa tcgcggtgca ttgtgtcgga ccagcgcctg cgtccgtgcc
     1141 agtaaaggac tggttggcaa ctgcattacc caagcaacgg taggcactat acgcacagta
     1201 gacagccaaa gtatcgtgaa caataatgtc gtcgccggct ctgacgttca actcaaagcc
     1261 aatctgaggt ggcccataga gaacacagtt gtcaagacga accgactgca gtccgttgtt
     1321 ggtcgcacct ttcatgagac attttccagc ggaggcactc attttgccac ttcttgtgcc
     1381 agggagcgcg tccttgtttg ccagagcaga agccttatcc gtgaccatcg gtgcaccagt
     1441 gtcataggct tctgtaaaaa cgcagttgta aaacttgacc cagttccgat tcatggcagg
     1501 cgaagccgga tcattgatca tgatcaaggt gttcataacg taagagctgc ccgcatcggg
     1561 actgaatatg aaattgttga acgtcacatc gtacgtatcc tggtacagca gcattccacc
     1621 gacggtgcta cttgggttga gtctgatatt tacaggtgtc gcagcctgaa taatcagttc
     1681 gccaacaata tcgcccagac caacaagccc tttgtcgatg cgaatattct ccgagtacgg
     1741 accgtttaca acgttaatca cgaaaggtgc cgtctgaccc gcgttcgtcc cgccagcaca
     1801 ccaactggat atcgcagacg tgatcgtgct aaacgtgttc acaccatccg gcgttgcatt
     1861 cgtgctatct accgtaacag gattccctgc ataggcacct gtagccaaaa ccagcatcaa
     1921 actgagaaag aaatacttct tcatcgaaac ttcctccaat agactcttta atatgactcc
     1981 ggtctcgtgt ggagacccca acaaacccat gccttctcag aaccacctcc ttccagcaac
     2041 attctcttcc ttggattcta gttcctatcc tctaccggtg tctctctttt ctctgatatc
     2101 gcagagtttt actgaacatg tgtagtttat ggaaaccaaa tttgcatgtc aagtgttctt
     2161 tttgtatatg tgaatttcat aacctgtgtt caaaaacatg cggaaatgga atggcaaatg
     2221 ccccgacaga ggctatcccc tctccaaaga gagagtggca ccagttcacg acaggcccaa
     2281 agaaccaccg ggaggcgaga aagaagaaaa acttgacatg aaccttgaaa gcaatataat
     2341 gcaaacatgt tcatcgtttg agtgcacata tctaccaaat ggagatttgg ttcacggact
     2401 gaagtcccag agatttggag gcaggagtag gagatggcat cacagaggga aacagagata
     2461 atcgaacgcc ctg
//
LOCUS       DAOQUC010000544         2470 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_51716_length_2470_cov_3.045963, whole genome shotgun sequence.
ACCESSION   DAOQUC010000544 DAOQUC010000000
VERSION     DAOQUC010000544.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2470
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51716_length_2470_cov_3.045963"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..435
                     /gene="rplD"
                     /locus_tag="PKH07_09840"
     CDS             <1..435
                     /gene="rplD"
                     /locus_tag="PKH07_09840"
                     /inference="COORDINATES: protein motif:HMM:NF012782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HNT35283.1"
                     /translation="PYRQRGTGRARQGSFRSPVRRGGGTVFGPHPRDYRKALPKKIRQ
                     EAVRIALSQRLREGSFSVVDSIEVPQPKTKTVAEVMKSFDLNGKILVVHDGRDGNLVL
                     SGRNLMGVTLIAARELNALVVLENERLLVTEPALQEIEQLWG"
     gene            426..713
                     /gene="rplW"
                     /locus_tag="PKH07_09845"
     CDS             426..713
                     /gene="rplW"
                     /locus_tag="PKH07_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012811930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HNT35284.1"
                     /translation="MGITQSPYNVIKGAVFTERSTGLKDENRYVFNVAFEANKVSIRR
                     AIEQIYRVHVEEVNTMVRQGKQRRLRYQLGRTPRSKRAIVRIRAGEKIDLI"
     gene            760..1584
                     /gene="rplB"
                     /locus_tag="PKH07_09850"
     CDS             760..1584
                     /gene="rplB"
                     /locus_tag="PKH07_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012174139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HNT35285.1"
                     /translation="MGIKTFRPVTPSQRYRTVPDFEEITKKSPVKSLTEAKPSKGGRN
                     SHGHVTMRHRGGGHKRRYRIVDFKRDKHGIPAKVEAIEYDPNRSARLALLLYADGERR
                     YILAPEGLVPGMTVVSGQKVEPQVGNAMQLANILEGTFVHAIELRKGQGAVLVRSAGG
                     YAQLMAKEGGYAHLKLPSTEVRLVPLECQATLGQVGNLLHNNVSIGKAGAQRWRGRKP
                     RNRGVAMNPVDHPMGGGEGKSSGGRHPCSPWGQIAKGLRTRRNRRTDRMIVRGRKR"
     gene            1626..1910
                     /gene="rpsS"
                     /locus_tag="PKH07_09855"
     CDS             1626..1910
                     /gene="rpsS"
                     /locus_tag="PKH07_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018046578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HNT35286.1"
                     /translation="MPRSVKKGPFIDLHLSKKFDVLNQTGEKRVIKTWSRRSTVMPYM
                     VGHTVAIHNGKKFIPVFLTENMVGHKLGEFAPTRTFRSHGGKTARVAKVH"
     gene            1962..2294
                     /gene="rplV"
                     /locus_tag="PKH07_09860"
     CDS             1962..2294
                     /gene="rplV"
                     /locus_tag="PKH07_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007191153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HNT35287.1"
                     /translation="MGATAKAKYVRISAKKARLVADMVRGRPLEEAKIALRFTDKKAA
                     RIFLKVLESAQANVETSTSLDIDQLVVKTVTVDEGFRLKRFRPCPQGRASRILKRTSH
                     ITVVLAEG"
     gene            2316..>2470
                     /gene="rpsC"
                     /locus_tag="PKH07_09865"
     CDS             2316..>2470
                     /gene="rpsC"
                     /locus_tag="PKH07_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012454030.1"
                     /note="forms a complex with S10 and S14; binds the lower
                     part of the 30S subunit head and the mRNA in the complete
                     ribosome to position it for translation; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HNT35288.1"
                     /translation="MGQKVHPYGLRLGIIKTWQSIWFADRKKYAQLLHEDIAIRALIK
                     ERLKHAAV"
BASE COUNT          608 a          572 c          780 g          510 t
ORIGIN      
        1 ccgtaccgac aaaggggtac tggtcgagcg cgacagggat cttttcgctc tccggtgaga
       61 cggggaggcg gtaccgtgtt tggtcctcat ccgagggatt accgcaaggc gcttccgaag
      121 aaaattcgtc aggaagcagt tcgaattgcc ttgtcacaac gcttgcgtga aggcagtttt
      181 tcggttgtgg actccatcga ggttcctcaa ccgaagacca agacggtggc agaggttatg
      241 aagagttttg acctgaacgg caagattctg gtcgttcatg acggtcggga tggcaatctg
      301 gttctttccg gtcgcaacct catgggagtg acattaattg ccgcccggga attgaacgct
      361 ctggttgtct tggagaacga aagactcctt gtcaccgagc cggcacttca ggagatcgaa
      421 cagctatggg gataacacaa tcaccgtaca acgtcattaa aggtgctgtc ttcacagagc
      481 gctcgacggg tctgaaggat gagaaccgct atgtgttcaa cgtggcgttt gaggccaaca
      541 aagtctcaat tcggcgtgct atcgagcaga tctatcgcgt ccatgttgaa gaagtgaaca
      601 ccatggttag acagggaaag cagcgtcgtc tccgctatca gctggggaga acacctcgaa
      661 gcaagcgggc gattgtccgg atccgagcgg gcgagaaaat tgacctgatc taagggtaga
      721 gccgggtgtc gtcacccgga gcatgaggag gagtcgatct tgggcatcaa gacattcaga
      781 ccggtgacgc caagtcagcg atacagaacc gttccagatt ttgaggagat taccaagaaa
      841 agtccggtga agtccctgac ggaagccaag ccatcaaagg gtggcaggaa ttcccacggg
      901 cacgtcacca tgagacatcg gggcggtggc cacaagcgaa gataccgtat cgtggacttc
      961 aagagagaca agcacggtat cccagccaag gtggaggcga ttgagtatga tccgaaccgt
     1021 tccgcgcgtc ttgctctact actttacgcg gacggcgaga gacgctatat ccttgcgccc
     1081 gaaggcttgg tgcctggaat gaccgttgtc tccggccaga aggttgagcc gcaggttggc
     1141 aatgcaatgc agttggccaa catcctcgaa ggcacgtttg ttcatgccat cgaacttcgc
     1201 aaagggcaag gagcggtttt ggttcgcagt gctggtggct atgcacagct gatggcaaaa
     1261 gaaggtgggt acgcccatct gaaattgcct tcaacagaag tccgactcgt gcctttggag
     1321 tgtcaggcga ctctcggaca ggtcggaaac ctgctgcaca acaatgtgag tattggaaaa
     1381 gcgggcgccc agcggtggcg tggtcgcaaa ccgcgaaacc gaggcgttgc gatgaatccg
     1441 gtagaccatc cgatgggtgg tggagagggc aagtcttctg gtggtcggca tccatgcagt
     1501 ccatgggggc agattgccaa agggttgaga accaggcgga accgacgcac ggacagaatg
     1561 atcgtgcgcg gtcgaaagag gtagccaggg cacagccctg agtgaggcag aaaggggaat
     1621 ctggaatgcc tcgttctgta aaaaaaggtc cgtttatcga tctgcatctg tccaagaagt
     1681 ttgatgtgct caatcagacg ggagagaagc gggtcatcaa gacgtggtcc aggcgttcga
     1741 cggtcatgcc ttatatggtc ggtcacacag tggcgattca taatggcaag aagttcatcc
     1801 ctgtgttttt gaccgagaac atggtgggac acaaactggg tgagtttgct ccgacgcgaa
     1861 ccttccggtc gcacggtggc aagacggcga gagtggcaaa agttcactaa cacgcggcgc
     1921 aggcatctcg gtgtcggtgc gcctgaagag gacaaagggc aatgggtgcg acagccaaag
     1981 cgaaatatgt gcggatatct gcaaagaagg caaggctggt ggcagacatg gttcgtggcc
     2041 ggcctttgga agaagcgaaa attgctttgc ggtttaccga caaaaaggcg gcgcggatct
     2101 tcctaaaagt gttggagtct gcgcaggcga atgtcgagac gtccacaagc ttggatattg
     2161 accagttggt ggtcaagacg gttaccgtgg acgaggggtt tcggttgaag cgatttcggc
     2221 cctgtccgca ggggcgtgcg tcgcggattc tgaaaaggac gagtcacatc acggttgtgt
     2281 tggcggaagg ctaagacgat agaggaggtg cccccttggg acagaaggtt cacccgtacg
     2341 gactgcggct tggaattatc aaaacctggc agtcaatatg gtttgccgac aggaagaaat
     2401 atgcacagtt gctgcatgaa gatattgcga ttcgggcttt gatcaaagaa cgtctgaagc
     2461 acgccgcggt
//
LOCUS       DAOQUC010000545         2467 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_51833_length_2467_cov_3.262852, whole genome shotgun sequence.
ACCESSION   DAOQUC010000545 DAOQUC010000000
VERSION     DAOQUC010000545.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2467)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2467)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2467
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51833_length_2467_cov_3.262852"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..471)
                     /gene="panC"
                     /locus_tag="PKH07_09870"
     CDS             complement(<1..471)
                     /gene="panC"
                     /locus_tag="PKH07_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HNT35289.1"
                     /translation="MRVLRTVAELRSCRKDLSRTGSSVGFVPTMGCLHEGHLSLMRTA
                     RQQNDSVWVSIFVNPTQFGPNEDFERYPRTESRDIELCRECGVEILFIPPAREMYPEG
                     SQTWVEVEHLPKALCGASRPTHFRGVTTVVAKLFNLVQPDRAYFGQKDAQQVIVL"
     gene            complement(560..865)
                     /locus_tag="PKH07_09875"
     CDS             complement(560..865)
                     /locus_tag="PKH07_09875"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HNT35290.1"
                     /translation="MDTATVEKNTVRITILDKTYSLRATMSQQHMQRVAAFVDEKVRK
                     LRHDFPEMPIQRLAILAAIQIADEHLTTAPMNQDNAGEIRQRINSLISHIDELLSKD"
     gene            complement(878..1078)
                     /locus_tag="PKH07_09880"
     CDS             complement(878..1078)
                     /locus_tag="PKH07_09880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35291.1"
                     /translation="MDVMEVRDGLRLLEEKMGSLLDQLEFLRQERDRLLSENDSLREE
                     RDAVRNQLEELLERIDRLTSNS"
     gene            1275..>2467
                     /locus_tag="PKH07_09885"
     CDS             1275..>2467
                     /locus_tag="PKH07_09885"
                     /inference="COORDINATES: protein motif:HMM:NF012475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HNT35292.1"
                     /translation="MKSFSLAVNTLTWLIACGILSVGAIAQVDIGTGWDRAAGRPAWA
                     SSTWGDNVAGNAVDASTYTAWNSLESRGFLWVDLGARMRIVAIGQDLSLPTKIGASTD
                     LIGFGSEGWHVASTVLSTTTCAGLLADTTVELNCRSVGLQSSGYIQTGNRSSWKDERA
                     SLSTFKAYQAGWRPKKGAADVRLQDFCLFQHGGKMYIASMMKDYCSQGIIIGRSDDLW
                     HWNEIGKAVTTRTAEDACMVWAPHVVEDAGVCYMFYTGVTCPQSGAWCQRILVATTTD
                     PDNPSTWQRNTSVQFDVAGTIQSWFRPSHAGAVWTDSGWADCRDPMVMKHGDTWYLFY
                     SGRDAGFGICGVATAPDILGPWVDHGAVLKTPDPTIPESCFVLEDPDGTFVMTLNHAA
                     GGGEVV"
BASE COUNT          518 a          613 c          713 g          623 t
ORIGIN      
        1 aaggacgatt acctgctgtg catccttctg accgaaatag gcgcggtcgg gttgcacgag
       61 attgaagagt ttcgccacga cggttgtcac gcctcggaaa tgcgtgggac ggctggcacc
      121 acagagcgct tttggcagat gttctacttc aacccaggtt tgactgcctt cggggtacat
      181 ctcccgggcg gggggaatga agaggatttc cacgccgcat tcacggcaca gctcgatatc
      241 ccgagactcg gttctgggat agcgctcaaa gtcctcgttg ggaccgaatt gggttggatt
      301 gacgaagatc gagacccaga cgctgtcgtt ctgttgtcgg gcagtgcgca tcagtgaaag
      361 gtgtccctcg tgcaagcagc ccattgtagg cacaaaaccg acggagcttc ccgtccgcga
      421 gagatccttt cggcacgatc tcagttcagc gactgttcgg agcactctca tggttgttct
      481 ccctcagggc tttgcattgc gaacatcgcc cattatcgtt tgggtacggt tgagtttggg
      541 ttgtcggcat ttcgggtgtt cagtcttttg agagaagctc gtcaatgtgg ctgatcagcg
      601 agttaattcg ctggcgaatc tctccggcgt tgtcctgatt catgggagct gtggtcagat
      661 gctcatccgc aatctgaatt gccgcaagga tggcaaggcg ctgaatgggc atttcgggaa
      721 aatcgtgacg cagtttgcgg actttttcat ccacaaaggc agcaacgcgc tgcatgtgtt
      781 gttgggacat tgtcgcccga agtgaatacg tcttgtccag gatggtgatc cgaaccgtgt
      841 ttttttcgac cgttgctgtg tccaaggcat gaacccctta agaattgctg gtcagccgat
      901 ctattctctc cagaagttcc tcgagctgat ttctcacggc gtctcgctcc tcacgaagac
      961 tgtcattctc tgaaagaaga cggtctcgct cctgacgcaa aaactcaagt tgatccaata
     1021 gcgaccccat cttctcctca aggagtctga ggccatctcg aacttccatt acgtccatgc
     1081 gtcttcacca tctcgacagc attacctaaa gttctatcgc aatctatacc ctttaacttt
     1141 tgtcttgtca agctgattga tgttcttttt cttctgtttg acagaaggcc aacagcagtg
     1201 gataatcggc aaaatctggg gaaaaggtgt cagggatttg tatatcgtgg gcacctccag
     1261 gaggcgaaac gctcttgaag tctttttctc tcgctgtgaa tactctgacg tggttgattg
     1321 cttgtgggat tctctcggtt ggtgctattg cccaagtgga tatcgggacg ggatgggatc
     1381 gcgcggcggg gcgacctgca tgggcgagtt ccacttgggg agacaacgtc gctggaaatg
     1441 ctgtggacgc aagcacttat accgcatgga attccttgga atcaagggga tttctttggg
     1501 tggatctggg ggcgagaatg aggattgtcg ccatcggtca ggatctctcc cttccgacga
     1561 agatcggtgc ctcaaccgac ttgattggat ttgggagtga gggatggcat gttgcttcaa
     1621 cggttctcag tacgacgact tgtgctggct tgttggctga taccacggtc gaactgaact
     1681 gccgcagcgt cggcttgcag tcttccggct acattcagac ggggaatcgg tcttcctgga
     1741 aggacgagag ggcttctctg agcacgttca aggcctacca ggcgggatgg agacccaaga
     1801 aaggcgctgc cgatgtgcgt ctacaggatt tctgtctgtt tcaacatggt ggaaagatgt
     1861 acatcgcttc gatgatgaag gactactgct cacaaggaat catcatcggc agatcggatg
     1921 acctgtggca ctggaatgaa atcggcaaag ctgtgactac gcgcacagca gaggatgcct
     1981 gtatggtttg ggcaccacac gtggttgagg atgccggtgt ctgctacatg ttctacacag
     2041 gcgtcacctg cccacagtcg ggggcttggt gccagagaat cctggtggca acaaccacag
     2101 atcccgacaa cccctcaacc tggcagcgga acaccagtgt tcagttcgat gttgccggaa
     2161 cgattcagtc ctggttcaga ccaagccatg ccggcgctgt ctggacagat tcgggttggg
     2221 cagactgtcg ggatccgatg gtcatgaaac acggggatac ttggtacctg ttttattccg
     2281 gacgtgatgc aggatttgga atctgcggtg tcgccacggc accggacatt ctgggccctt
     2341 gggttgacca tggcgctgtc ctgaagacac cagatcccac gataccagaa tcctgttttg
     2401 tgcttgagga tccggatgga accttcgtca tgacgctcaa tcacgcggca ggcggtggcg
     2461 aggttgt
//
LOCUS       DAOQUC010000546         2466 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_51872_length_2466_cov_3.620904, whole genome shotgun sequence.
ACCESSION   DAOQUC010000546 DAOQUC010000000
VERSION     DAOQUC010000546.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2466)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51872_length_2466_cov_3.620904"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..477)
                     /locus_tag="PKH07_09890"
     CDS             complement(<1..477)
                     /locus_tag="PKH07_09890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35293.1"
                     /translation="MSSILKALRKREQARSGEQSPFQGGGYSPESQPVERLMPWIKAC
                     LLIALSLCLLSGGLWLGFKAFSQLKRPESPATPQRESLEEIQEIIRAQIAQALPTMTF
                     TPTSTPTPIVPTAAPPEPTETLKPTRTPTAVQRMPIEQVRKPIPPSQTPTALPKTTP"
     gene            complement(474..>2466)
                     /locus_tag="PKH07_09895"
     CDS             complement(474..>2466)
                     /locus_tag="PKH07_09895"
                     /inference="COORDINATES: protein motif:HMM:NF024793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNT35294.1"
                     /translation="YLFLTEKHREALAHLVYGIEQRRGFIVITGEVGTGKTTICRSLL
                     TQFGEDTRIALVLNPMLSEVELLESILNEFYISIAEHTKKSLLEQLDAFLIQQHSQGR
                     NVVLVIDEAQNLPLETLEQIRIISNLETEKVKLIQIVLLGQPELSEKLASPQLRQLEQ
                     RVSVRFHLTPLTEEETREYIQHRIMVAGGQGTIHFDAQALKFISDKTQGIPRKINVLC
                     DRALLVAFVEGAGRITEAIVKQAFGEIQSERPSRKTLSKAKSAEPDLSILGKAAPLDS
                     PRVNLLRTVVVLILILLVALLGVNFWLVLTVRDLSRPNVVSHADSLEALSDSEPAIES
                     GNKSDDRLESTVKDTAIPSTEIPEVSSLNESAVPDTLAAQVQIGEATPELPQPHLVQD
                     QTSESLLSPKGELDGEVLEEPTSEPVRVQATALPEPVSASAERAIATGEEVFDSEGVF
                     RAHRPESAPWAVRATLCRVWDASEEQLAEAFKQASLQSSATPFLALQNGFVEESFVLD
                     ATRVRGIGIPCILWSDTEEPAVLLSVQDDQATLAWASLGKFKTKWGELLTDWSGNVTY
                     IVPKSWRLEENWRPGVDASKAIFRMQNALREMGYFSYTPNGNYGSKTEEAVRRFQADR
                     GLTPDGIVGQETYIALKSAMELGVPHLSRADTQETERTP"
BASE COUNT          556 a          648 c          696 g          566 t
ORIGIN      
        1 gggtgtggtc ttcggaagtg cggtcggcgt ctgtgaaggg ggaatgggtt tgcgaacctg
       61 ctcaatgggc attctctgaa cggcagttgg agttcgagtt ggcttgagcg tttccgtcgg
      121 ttcaggcgga gctgccgttg gaacgatggg tgttggggtt gaggtcggag tgaaagtcat
      181 cgtcggcaac gcttgggcaa tctgggcacg gatgatttcc tgaatttctt ccagagattc
      241 cctctgggga gtggcagggg attccggcct cttgagttga gagaacgcct tgaaacccaa
      301 ccagagccca ccggagagaa gacagaggga tagggcgatg agcaaacagg ctttaatcca
      361 aggcatgagc ctctcaacag gctgagactc gggcgaatag ccaccacctt gaaagggcga
      421 ctgctctcct gagcgtgcct gttcgcgctt gcgaagtgct ttgagaatgg aactcatggt
      481 gttctttcgg tttcctgtgt gtccgctcgg gaaagatgag ggactcccaa ttccatcgcc
      541 gacttgagcg caatgtacgt ctcctgcccg acaataccgt caggagtcag ccctcgatct
      601 gcctgaaacc gcctcacggc ttcttcggtt ttgctcccgt agttcccgtt gggtgtatag
      661 gagaaatacc ccatctcacg caacgcgttc tgcattcgga agattgcttt gctggcatca
      721 actccaggac gccaattctc ttcgagtcgc caggacttgg ggacgatata ggtaacgttg
      781 ccagaccagt ccgttagaag ttcaccccat ttcgtcttga atttgccgag cgaagcccac
      841 gcaagagtgg cttgatcatc ctggactgag agaagaacgg caggctcttc ggtatctgac
      901 cacagaatgc acggaatgcc gatgccacgc actcgggtgg catccaagac gaaggactct
      961 tcaacaaatc cgttctgaag agcaagaaaa ggggttgctg aagactgaag actggcctgt
     1021 ttgaatgcct cagccaattg ttcctctgag gcgtcccaaa ctcgacacag tgtcgccctg
     1081 acagcccagg gagcagattc gggacggtgc gctcggaaca caccttcaga atcaaaaacc
     1141 tcttcaccgg tcgcgattgc ccgctctgcc gacgctgaga ctggctcagg cagagcggtt
     1201 gcctggactc gaaccggttc tgatgtaggc tcctccaaga cttctccatc caactccccc
     1261 ttgggagaaa gcaaagactc cgaggtttga tcctggacaa gatggggctg cggaagttcg
     1321 ggcgttgcct cgccgatctg aacttgagcc gcaagagtgt caggcacggc agattcgttg
     1381 agtgagctca cctcaggaat ctcggtagat ggaatagctg tgtccttgac agtggattcc
     1441 aaacggtcat cagatttgtt gccggactca atagccggtt cagagtcaga caacgcctcc
     1501 aatgagtcag cgtgtgatac cacattgggt ctgctcagat cgcgtaccgt cagcacaagc
     1561 cagaaattga ctcccagcaa agccacaagc agaatgagaa tcagtacgac aacagttctc
     1621 agcagattca ccctggggga atccagaggc gcagccttgc ccaggatgct caagtcgggt
     1681 tctgcagact ttgcctttga gagagttttc ctgctggggc gttcgctctg aatctctcca
     1741 aaggcctgtt tgacgatggc ttcagtgatg cgacctgctc cttccacaaa cgcgacaaga
     1801 agagcacggt cacaaagcac gttgatcttt ctcggaatcc cctgggtctt gtcgctgatg
     1861 aacttcagcg cctgggcatc gaaatggatg gtgccctgtc cgcccgccac catgatgcga
     1921 tgctggatgt actcgcgtgt ttcttcctca gtcagtggag tcaggtgaaa tcgaacggaa
     1981 acgcgctgct cgagctgccg cagttggggg gatgccagtt tctcagacag ctccggctgt
     2041 cccaagagca cgatttgaat cagtttgacc ttctctgtct cgagattgga gatgatccga
     2101 atttgctcca aggtttccaa aggaaggttc tgcgcttcgt caatcaccaa aaccacattg
     2161 cgcccctgag aatgctgttg gatcagaaaa gcatccagtt gttccaacag acttttcttg
     2221 gtgtgttcgg cgatggagat atagaactcg ttgaggatgc tctcaagcag ctccacttca
     2281 gaaagcatgg gattcaggac aagggcgatt cgggtgtctt caccaaactg ggtcagcaga
     2341 gagcgacaga tggtggtctt cccggttcca acctctcccg tgatgacgat gaatccgcga
     2401 cgctgttcga taccgtagac cagatgcgcc aaagcttccc ggtgcttctc cgtcaggaaa
     2461 aggtat
//
LOCUS       DAOQUC010000547         2466 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_51886_length_2466_cov_2.927416, whole genome shotgun sequence.
ACCESSION   DAOQUC010000547 DAOQUC010000000
VERSION     DAOQUC010000547.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2466)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51886_length_2466_cov_2.927416"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            84..317
                     /locus_tag="PKH07_09900"
     CDS             84..317
                     /locus_tag="PKH07_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009509129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35295.1"
                     /translation="MGHPTKVQLIQRKKGVSQYYINFPTVLAEAMEFQKSETVEWIVA
                     DKANLILHRKHTPASPVDVKKKTDRLCSMRSTD"
     gene            complement(866..1417)
                     /locus_tag="PKH07_09905"
     CDS             complement(866..1417)
                     /locus_tag="PKH07_09905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35296.1"
                     /translation="MTDLCLSDAEERFFIAEVGFDIPAPEVILEEFLERELSICADEI
                     SWFAIEQFGVFSKSIRERSDNHKSQIMPVACWAPSDGSDGFDAEVVLFATGICVDCLP
                     GSIRVLSQLLWGGQLWSVDSVSTLGLLLVWESVYFGVCPDSPDHGGTFRQAGEKGPVG
                     IASINYSHHGTRVNGRVGIQLLA"
     gene            complement(1646..2197)
                     /locus_tag="PKH07_09910"
     CDS             complement(1646..2197)
                     /locus_tag="PKH07_09910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35297.1"
                     /translation="MQVRFTISLALLVGLLLSPQHGLGNAGTLVYVAALEAATEDGKT
                     TWVPTLYAIDVEQSKIDRKTPLPSSGSPVECRGIFHGRIEVEQHDGIVSLSTRPGNRV
                     TLVTAFNAETLSVLEQIRRDGIADPTVPREREPTLLKPEWLSMTRTERLLSVSGLWPQ
                     SGFGGAVEESGIEVWEDFWGLCV"
     gene            complement(2212..>2466)
                     /locus_tag="PKH07_09915"
     CDS             complement(2212..>2466)
                     /locus_tag="PKH07_09915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35298.1"
                     /translation="ISEGRYVFETYYHDRQTATQRTWPETSDGDASVPNREDLAYYGY
                     THGEHLMRQVTGETWAARVQAYNGVQRLREYKRTRPWLNQ"
BASE COUNT          613 a          736 c          574 g          543 t
ORIGIN      
        1 gtgaagtgat tttgatgttg catttgagag gggaagaatg atagatttat atctagtata
       61 tatactagat taaaggagaa gccatgggcc acccgaccaa agttcagttg attcaaagga
      121 agaaaggcgt cagtcagtac tacatcaact ttccgacagt gcttgcagaa gcaatggagt
      181 ttcaaaagag cgaaacggtt gagtggatcg ttgccgacaa ggcaaatctg atccttcatc
      241 gcaagcacac gccagcgtcg ccagtggatg taaaaaaaaa gaccgacaga ctctgctcga
      301 tgaggtcgac agattgatct cccagtgtgc cgtggcaggc ggtgggcagg ctcacaagaa
      361 tcgcatcaaa gagcacatgt tgagttctct tgtatgcttg ggaagacata cggtgaccgg
      421 acatctggcg acggcgggaa gacagttcca ggactggact ggggcgtatc gtctgtattc
      481 ccaagacagg ctcgatagta ccttgctgtt tgaacccatt ctgcgctcat tggtgcagca
      541 tgttcaggca gagcatcccg tcgtggtggc agtggacgat accaggttga agaagagcgg
      601 tcgcaaaata ccaggagtcc aatataccag ggatcctttg gggcccccgt tcaacgtgaa
      661 tttcatcctc gcccagcgat tccttcagat gtcaattgca tggtcctgcg atgagcggca
      721 agtccggatg attccagtag acttccttca cgccccaaga ccacagagac cacccaagaa
      781 tgcttcccaa gagcaacttc agcaatatcg cgacgcttct cgacaagcgg ctttgccaca
      841 cgttgcggcg caaagactgg cattactacg caagcaattg gatgccgacc cgtccgttca
      901 ccctcgtccc atggtggctg tagttgatgg aggctatacc aaccggacct ttctctccag
      961 cctgcctgaa cgttccaccg tgatcgggag aatccgggca gacgccaaaa tatacgctct
     1021 cccaaaccaa caaaagccca agggtagaca cgctgtctac ggaccacaac tgcccacccc
     1081 agagcaactg cgacaagact ctgatactcc ctggcagaca gtcgacgcat atgcctgtgg
     1141 caaacaacac cacttccgca tcaaaaccat cgctcccatc agatggcgcc caacaggcaa
     1201 caggcatgat ctgcgacttg tggttatcgc tccgctctcg tatagacttc gaaaagactc
     1261 caaactgctc tatcgcaaac cagcttatct catctgcaca gatactcaac tcccgctcga
     1321 gaaactcctc cagtattacc tctggcgctg ggatatcgaa accaacttcc gcgatgaaaa
     1381 aacgctcctc ggcgtcggac aggcacaagt ccgtcatccc cagtccgttc acaccgctcc
     1441 agcgctctcc gtggccgctt acgcctcttt gctcaccgcg gcttgctccc tctcacaact
     1501 gcatcaactc aacaaccaac tcccgccagc caaatggtcc cacaagaacc ctcaaagact
     1561 ctccacgcag aacttgatag accgcctcag aaacgacctc tggagtgatg ccattgtttc
     1621 ttcacacttg cagaccaaca ccacctcata cacaaagtcc ccaaaaatcc tcccagacct
     1681 ctattccgct ctcttctacg gcgcctccaa aacctgattg tggccaaagt ccagatactg
     1741 aaagaagcct ttcagtacga gtcatggaga gccattccgg cttcagcaac gttggttccc
     1801 gctccctcgg aaccgtggga tcggcgattc catcacgtct gatttgttcg agaaccgata
     1861 gtgtctcagc gttgaaagcg gtgactagcg tgactctgtt tccaggcctc gtcgagagcg
     1921 atacaatgcc gtcgtgctgc tcgacctcga tccgtccatg gaatatgccc ctgcactcta
     1981 ctggagaccc tgaagacggc agaggtgtct tcctatcaat cttgctttgc tcaacatcaa
     2041 tagcgtagag tgtcggtacc caagttgtct taccgtcctc cgtagcggct tccagggctg
     2101 caacataaac gagcgtgcca gcattcccca acccgtgttg tggtgacaac aacaatccca
     2161 cgagcagggc caaagaaata gtgaaccgaa cttgcatatt ctgctctccc ttcactggtt
     2221 aagccaaggt cgcgttctct tgtactcacg caaccgctga acgccattgt atgcttggac
     2281 ccgcgcggcc caagtctccc ctgtaacttg tcgcatcaaa tgctctccat gggtgtaccc
     2341 gtagtacgcc agatcctccc tgttcggaac cgaagcatcg ccgtccgatg tctccggcca
     2401 cgtccgttgc gttgcagtct gcctgtcatg atagtacgtt tcgaatacgt acctgccttc
     2461 ggagat
//
LOCUS       DAOQUC010000548         2465 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_51916_length_2465_cov_3.565975, whole genome shotgun sequence.
ACCESSION   DAOQUC010000548 DAOQUC010000000
VERSION     DAOQUC010000548.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2465)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2465)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2465
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51916_length_2465_cov_3.565975"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..526
                     /locus_tag="PKH07_09920"
     CDS             <1..526
                     /locus_tag="PKH07_09920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35299.1"
                     /translation="TLTIAPETGSEKVRRQLGKLLTDADILNTASRAIEAGIPSLKLY
                     FLAGVGDLLQDESGVSGESAAIVSLVREIAQRRQEKGSMVVGLSPLVPRYGTPLQSKP
                     LPDRRHVQQVISAVRHGLRDFSGISVRAGGWFEAATEWILSHATAEMSETLMRAGVDT
                     QNRRAILAEEIAKR"
     gene            complement(749..>2465)
                     /locus_tag="PKH07_09925"
     CDS             complement(749..>2465)
                     /locus_tag="PKH07_09925"
                     /inference="COORDINATES: protein motif:HMM:NF019552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HNT35300.1"
                     /translation="LGWPVTREAVLESLCEIARTSTVKDGLTGERGLSGYSAIFPFLF
                     SDIIARFDALDSTLIDELFRRHPALGKTWRFFIDTWCLEKFYPCVGDCGRFGAIFPNF
                     VGVSLNVPVRFTSPSGFSFMWRLYEITGDPDYVKVIYKANDYKTADLPHDLGEKDPDA
                     FQQKVQKVIDQTGQNLTTGDSLFPQWGISVSRSGQGDFQNALWIMNNSSGNHGHLNAL
                     NIGLYARGTDLLPDFGYPPVGYGGWNAPKATWYLETAAHNTVVADAVAQQRADGAFAL
                     WCPGKELDLVKASAAPSAGCEIYQRTLLKVDISDEDFYVVDLFQIVGGSEHAYFLSSG
                     LGKAQATGLSLEEEVPAPFPNLTRNFRGQSSPAQGWTLDWCLRDESESRSKDAPPLHL
                     SYIGLTENASVSLGECWIDTSNSFGGREEWLPRLMVRRRSETPGLRSDFLGILQPYDR
                     EPFIISAIRQGLTYDPHREALVVDLVDGRRQIFLFSPVSGSPIEPKLVLPRVRTDAEF
                     VFLSFHRDRLEQAGLYNGTRLEIGRALVSLTADADVVPYAELSRAGGKLKVISKNDIA
                     VKIKP"
BASE COUNT          576 a          683 c          675 g          531 t
ORIGIN      
        1 gacgttgacc attgcgccgg agaccggcag cgagaaggtc aggcggcagc tcgggaagct
       61 tctgaccgac gcagacattc tgaacaccgc ctctcgggcg attgaggcag gaatcccatc
      121 gctcaaactg tattttctcg caggtgtggg tgaccttctt caggatgagt ccggtgtgtc
      181 aggagagtct gcagccatcg tctcattggt acgcgagatc gcacaacgcc gacaagagaa
      241 ggggagtatg gtcgtgggtc tttcgcctct cgttcctcga tatggaacgc cactgcagtc
      301 gaagcctctt cccgacagac gccatgttca gcaagtcatc tcggccgttc gtcacgggct
      361 gcgtgacttt tccggaatct ctgttcgtgc agggggttgg tttgaagctg caaccgaatg
      421 gattctgagc cacgcgacgg cggaaatgtc cgaaacactc atgcgcgcag gagtggacac
      481 acaaaaccgc agggcgattc tggcggaaga gatcgccaag agatagatca actcggcatg
      541 tgcaggcgca atggtcatac ccactcgtgc gtggtgcttg ctcctgcaag agaacctgcc
      601 tcttctgaag tctgattccc cgggagttgg aatgcagtca gttccacaat gactgcccaa
      661 gttttcctga caatgatgac aaggaatcag actgcacttc tcccccgcaa gaaatgctct
      721 ggtcttctgg ctgagccgat cgtgtgtctc aaggctttat cttaaccgcg atgtcgtttt
      781 tggagatgac cttcagtttg ccacctgcgc ggctcagttc cgcgtaggga acgacatctg
      841 catctgcagt cagagagaca agggctctcc cgatttcgag tcgagtgccg ttgtataggc
      901 ctgcctgttc gagtcggtcg cggtggaagg agaggaaaac gaactccgcg tcagttctca
      961 ctcttgggag cacaagtttt ggctcaattg gagacccgga gacaggggag aaaaggaaga
     1021 tctgccgcct tccatccaca aggtcaacga ccagggcttc tctgtgcgga tcgtacgtca
     1081 ggccttggcg tatcgccgat atgatgaacg gttcacggtc gtacggctgc agaattccca
     1141 ggaagtccga acgaagacct ggcgtttccg atcttcgtcg aaccatgaga cgaggcagcc
     1201 attcctctct gccgccaaag gagttggaag tgtcaatcca gcattcgcca agagagacac
     1261 tcgcattctc ggtcaaaccg atgtacgaaa gatgcagagg cggcgcgtct ttcgatctgc
     1321 tctcggattc atcccgcaga caccagtcca atgtccaacc ctgtgcagga gaactctgtc
     1381 ctcgaaagtt tctggttaga ttcggaaagg gagctggcac ctcttcttcc agacttaagc
     1441 ctgtggcctg agcttttcct agacccgacg agagaaagta ggcgtgttcc gaaccaccga
     1501 caatctgaaa gagatccacg acgtagaagt cctcgtcgga gatatcaacc ttcaaaagag
     1561 tccgctgata gatctcgcaa ccggcactcg gcgccgcgct ggctttcacc aagtccagtt
     1621 ctttcccagg gcaccaaagc gcaaaggctc catcagccct ttgctgtgca accgcgtcag
     1681 cgaccaccgt attgtgcgcc gccgtttcaa gataccatgt tgctttcggg gcgttccaac
     1741 caccgtaccc caccggcgga tagccgaaat caggcaggag atctgtgcca cgcgcataca
     1801 aaccaatgtt gagcgcgttc aggtgaccat ggttcccaga ggaattgttc ataatccata
     1861 gggcgttctg gaagtccccc tggcctgatc tcgaaactga aataccccac tgcggaaaca
     1921 gcgaatcgcc agttgtcaga ttctgtccgg tttggtctat gactttctgc actttctgct
     1981 gaaaggcgtc tggatctttc tcccccaagt catgagggag atcggcggtc ttgtagtcat
     2041 tcgccttgta gatgaccttg acgtagtctg ggtctccggt gatctcgtac agcctccaca
     2101 tgaaagagaa tccagaagga gaggtgaatc gcactggaac attgagactt actccgacga
     2161 agttggggaa aatcgcaccg aagcggccac agtcccccac acaggggtag aacttctcga
     2221 gacaccaggt gtcaatgaag aaccgccagg tcttgccgag cgctggatgc cgccggaaca
     2281 gctcatcaat aagcgtcgag tcaagagcat caaagcgggc aatgatgtcg gagaagagga
     2341 atgggaatat agccgaataa ccagagagtc ccctctctcc agtcaggccg tccttcactg
     2401 tcgaggtacg ggcgatctca cacagtgact caagcaccgc ctcacgagtc acaggccatc
     2461 caaga
//
LOCUS       DAOQUC010000549         2462 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_52017_length_2462_cov_3.084753, whole genome shotgun sequence.
ACCESSION   DAOQUC010000549 DAOQUC010000000
VERSION     DAOQUC010000549.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2462)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2462)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2462
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52017_length_2462_cov_3.084753"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /locus_tag="PKH07_09930"
     CDS             complement(<1..254)
                     /locus_tag="PKH07_09930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35301.1"
                     /translation="MNQAIGLPFWGIVTRVRLGLSMMIWMLFCHYSPRNKQVAGAKNS
                     MLASTTAIGFSPSLDMVGGLWHDQNQVHGGKLRYERGLED"
     gene            165..2291
                     /locus_tag="PKH07_09935"
     CDS             165..2291
                     /locus_tag="PKH07_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004501907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HNT35302.1"
                     /translation="MTEEHPNHHRESQSHTSDNSPEGKPNRLIHEKSPYLLQHAYNPV
                     NWYPWGEEAFREASRQNKMVFVSIGYATCHWCHVMERESFEDETTAEILNGHFVSVKV
                     DREERPDIDAIYMDAVHSMGQQGGWPLNIFLTPDAKPIAGGTYFPSRSMHGRRSFTEV
                     LAILIEVWSDRRKEALDAADDLVTHLRDAYSPMSGTELPDRQCVERAVGLYERLFDSR
                     FGGFRTDSANKFPPNMALRLLMDYFYRSGRENCLDMCVKTLVAMKTGGIYDQVGGGLC
                     RYSTDHQWLVPHFEKMLYDNALFLSALADCYQLTQNRFFRQAAYDVMNYIRRDMRTPE
                     GAIASAEDADSEGKEGAFYLWSLSEFRTVTKDDSDVMENFWGLAEEGNFEGMNILHEE
                     PSSRSESHALKAGQEMEKIIGRNRERLLTRRNTRPRPLRDDKVLTSWNCLYIQALARA
                     GRAFHDVEIIREAELIHSFVNVFLVNSEGRLLRRYREEESAIYGYLSDYSEMALASLE
                     LFRSTFQLRYVESAVRFVNEAVRLFLSDSGSFYETGSDAPALIRRTINAYDGVEPSGN
                     SSMAKALCALAFLGIDSVRYLELAEGVFRSQKDQIQRNPMSCPALLQAFLFFALPKTE
                     VVLIGSQESHQMQESLQWLNQDYRSDLVVVVATPENLEQMKKMIPLLEGKKSQGDYVG
                     YVCRDMTCQKPCQSWADLKAILTTMK"
BASE COUNT          601 a          561 c          675 g          625 t
ORIGIN      
        1 tcatcttcaa gacctctctc atacctgagt tttcctccat gaacctggtt ctgatcatgc
       61 cacagaccac ccaccatgtc cagcgacgga ctgaagccga tagcggttgt gctggccaac
      121 atgctattct tggccccagc cacttgcttg tttcgaggtg aataatgaca gaagagcatc
      181 caaatcatca tagagagtcc caatcgcaca cgagtgacaa ttccccagaa gggaagccca
      241 atcgcctgat tcacgagaag agtccctatt tgcttcagca cgcatacaac ccagtgaatt
      301 ggtacccatg gggagaagaa gcctttcgag aagcgagccg tcagaacaag atggtctttg
      361 tttccatcgg atatgctacg tgccattggt gccatgttat ggagcgagag tcttttgagg
      421 atgagacaac tgcagagatt ctgaatggcc attttgtttc agttaaggtg gatcgtgagg
      481 agaggccaga tattgatgcc atctacatgg acgcagttca ttcgatgggg caacagggtg
      541 ggtggcctct gaacatattc ttgactcctg acgccaagcc aattgctggc gggacatatt
      601 tcccgtctcg ttcgatgcat ggacggcgga gtttcactga agtactggcg atcctcattg
      661 aagtgtggag cgacagaagg aaggaagctt tagatgcggc agatgatttg gtgacccacc
      721 tcagagatgc gtatagtccg atgtctggca ccgagctgcc ggaccgtcag tgcgttgaac
      781 gagcagttgg tctgtatgag cggcttttcg actcacgttt tggtggtttt cgtactgatt
      841 cagcgaataa gttccctcca aatatggcgc tcagactact catggactac ttctatcggt
      901 ctggcaggga gaattgtctg gacatgtgcg tgaagacact tgttgcaatg aagaccggtg
      961 gaatctacga tcaggttgga ggaggcttat gccgttactc cactgatcat caatggttgg
     1021 ttcctcactt cgagaagatg ctctatgaca acgcactctt tctctctgct ttggctgact
     1081 gttaccagct gacgcagaac cgattctttc gtcaggcagc ttatgatgtg atgaactata
     1141 tccgcaggga tatgagaact cctgaaggag ctattgcgtc tgctgaagat gcggattcag
     1201 agggcaaaga gggtgctttc tacttgtggt cgctcagcga gtttcggact gtgacgaaag
     1261 acgattcgga cgtgatggag aatttctggg gattagccga agagggcaat ttcgaaggaa
     1321 tgaacattct tcatgaagag ccttcctctc gttcagaatc tcatgctctg aaagcggggc
     1381 aggagatgga gaagatcatt ggccgcaaca gagaacggct gctcaccaga cgcaatactc
     1441 gccctcgacc tcttcgagat gacaaagtgt tgacgtcatg gaactgtctg tacatccagg
     1501 cactcgcgag agcaggacgc gccttccacg acgtggagat catccgtgaa gcggagttga
     1561 tccattcttt tgtaaacgtc ttcctggtca actccgaggg cagactgctt cgaaggtatc
     1621 gagaagagga gagtgccata tatgggtatc tgagcgacta ttcagaaatg gcgctggcgt
     1681 ctctggaact gtttcgaagc acttttcagc tccgatacgt ggagagcgcc gttcgattcg
     1741 tgaatgaagc tgttcgtctg tttctgtcgg actctggatc attctatgag acaggttctg
     1801 atgctccagc tctcatacgg agaaccatca atgcctatga tggtgtggag ccctcgggca
     1861 acagcagcat ggcaaaggcg ctatgcgctc ttgcgtttct gggtattgac tcagtgcgat
     1921 atctcgaact ggcagaggga gtgtttcgat cacagaagga tcagatccaa cgcaatccga
     1981 tgagctgtcc ggcactgcta caggcctttt tgttttttgc tctaccgaaa acagaagttg
     2041 tgttgattgg atcacaagag agccatcaga tgcaagagag tctgcagtgg ttgaaccagg
     2101 actatcgcag tgaccttgtt gtggtggtgg caactccgga gaatctcgaa cagatgaaaa
     2161 agatgattcc tttgcttgag ggcaaaaagt cccaagggga ttatgtcggg tacgtttgca
     2221 gggatatgac atgccagaaa ccgtgtcaaa gctgggctga tctaaaagcc attctgacga
     2281 cgatgaagtg aagtccggtg aattgaggcc ttgcaggcag atccagtgac ggcgaacaga
     2341 gtcacttctt tgttgcttgc tgtgacttct gcgaggctgt tgactgatac tttgcctttc
     2401 cgagaaatct accagaactg tcatagaagc gagaagagcc tgactgttcg tcaatgctct
     2461 tg
//
LOCUS       DAOQUC010000550         2460 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_52060_length_2460_cov_3.680249, whole genome shotgun sequence.
ACCESSION   DAOQUC010000550 DAOQUC010000000
VERSION     DAOQUC010000550.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2460)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2460)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2460
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52060_length_2460_cov_3.680249"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..926)
                     /locus_tag="PKH07_09940"
     CDS             complement(<1..926)
                     /locus_tag="PKH07_09940"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HNT35303.1"
                     /translation="MSQLLPGNQDSKKPVSSNEQMSAGANIAQTPAGAGLLGIDVGTS
                     GIKAVVFDLNGKTIASSFQEYPLLTPKPQWAEQNPEDWWQATCLACQQLLAADGVSAE
                     RILGLSFSGQMHGSVFLDKDGSVVRPCILWCDARTGEECRIITEKAGRDNLHAWTSNP
                     ALAGFTAPKAIWLQRHEPENFDKVRTLLLPKDYVRFRFTGELSTEVSDNAGTLLFNVG
                     TKKWAAELLKVLGIPEGWMPPIYESFEITGRVTEIASKQTGLKVGTPVVAGGADNPCG
                     AIGTGVVSEGQMFSSIGSSGVVFAPCSGLKVDP"
     gene            complement(923..2098)
                     /gene="recF"
                     /locus_tag="PKH07_09945"
     CDS             complement(923..2098)
                     /gene="recF"
                     /locus_tag="PKH07_09945"
                     /inference="COORDINATES: protein motif:HMM:NF000823.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HNT35304.1"
                     /translation="MKNQPELRALKKNMFLNQIKVRNFRNIEAADLKLGTGYNVFLGA
                     NAQGKTNILESILFLATSTSHRTLREKYLIRHGQDVAYIQGEIDTRRGPVRLESGLSL
                     EKKILKVQGRPLSRVGDLYGHLRVVLFAPEDLFLIKGSPRLRRKFLDLAIAQVQPDHV
                     PLLQSYRKLLRQRQTILRQRADHPGLNAQLDAWEQQLAIPAAEIVSRRRSLVTKLTER
                     TRHLAEQLSGGADCLELIYRSSVGDFDPAQGVPVFLERFRASRERDRQQKQSTVGPHR
                     DELSFLLHGRDVAVFGSQGQVRAASLVLRLAEASLIETEAGEAPVLLIDDVIFEMDAG
                     RRTRFFALLPKASQGLVTATQQQDVAPLIDAGHVFEVKMGTFTALDLDGRTPQGGTS"
     gene            2192..>2460
                     /locus_tag="PKH07_09950"
     CDS             2192..>2460
                     /locus_tag="PKH07_09950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35305.1"
                     /translation="MSQSRSGMCSSDKAQVVEAVVLSAKRLEGPYLRLAVRLPEPTLC
                     CPGQFFNLKTGGEPEPLWRRPLSVFDQNEDTVEFYIRIVGRGTEWL"
BASE COUNT          534 a          678 c          675 g          573 t
ORIGIN      
        1 ggatccactt tgaggccaga gcatggagca aacaccacgc ccgaagaacc gatactggaa
       61 aacatctggc cttcagagac aactcctgtt ccaatggccc cgcagggatt gtctgcgcca
      121 cctgccacaa ccggtgttcc gactttcaat ccagtctgct ttgaagcgat ttccgtcacg
      181 cgtccggtga tctcaaagga ttcgtatatc gggggcatcc acccttcggg gatacccaaa
      241 accttcaaaa gttctgctgc ccatttcttt gtgccgacat tgaacagcag cgttcccgcg
      301 ttgtcggaca cctctgtgga tagttccccc gtgaagcgga atctgacgta gtccttgggc
      361 agaaggagcg ttcgaacttt gtcgaagttc tccggttcat gtcgttggag ccaaattgcc
      421 ttcggagctg tgaaacctgc caatgcggga ttggaagtcc aggcatgaag attgtcacga
      481 cctgccttct cagtgatgat tcgacactct tcccctgttc gggcatcgca ccaaagaatg
      541 cacggtcgaa ctacactgcc gtccttgtcg agaaagacag agccgtgcat ttgacccgag
      601 aaactcaagc caagaattct ctccgcgctg actccatcag cggcgagcag ctgctgacag
      661 gcgagacacg ttgcctgcca ccagtcttcc ggattctgtt ccgcccattg cggtttggga
      721 gtaagaagtg ggtattcctg aaaggatgag gcaatcgttt ttccgttcag gtcaaaaaca
      781 accgccttga ttccagaagt tccaacgtca atgccgagca ggccagcgcc tgcaggcgtt
      841 tgggcgatgt tcgctcctgc agacatttgt tcattggaag aaacgggttt cttcgaatct
      901 tggtttcccg ggagcagttg gctcatgatg ttccaccttg cggtgtccgc ccatccaaat
      961 cgagggctgt gaatgttccc atctttactt caaaaacgtg cccggcgtca atcagaggcg
     1021 ctacgtcctg ttgctgtgtg gcggtgacaa gaccctgcga cgccttcggc aggagcgcga
     1081 agaaccgtgt cctgcggccg gcatccatct cgaaaatgac atcatcgatc agcagaaccg
     1141 gcgcttcccc agcctcggtc tcgatcaagg atgcctccgc gagtcgaagc accaggctgg
     1201 cggcgcgaac ctgtccctga gagccgaaaa ccgcgacatc cctgccgtgc agcagaaaac
     1261 tcagttcgtc gcgatgcgga ccgaccgtgg actgtttctg ctgacggtcg cgttcacgcg
     1321 aagcccgaaa ccgttcgagg aagactggta ccccctgcgc cgggtcaaaa tcccccaccg
     1381 aagaccggta gatcaactcc aggcagtctg caccaccgga cagctgttcc gcaagatggc
     1441 gggtccgctc cgtaagtttt gtgacgaggc ttctgcgtct ggagacgatt tccgcagcgg
     1501 ggatagccag ttgttgttcc caggcgtcca gttgcgcgtt gagcccggga tgatcggcac
     1561 gttggcgcag aatggtttgt cgttgccgaa gcagtttgcg gtaactctgg agcaggggca
     1621 catgatccgg ttggacttgt gcaatggcca gatcaaggaa tttgcgtctg agtcgtggac
     1681 ttcctttgat gagaaagaga tcctcgggtg caaagagcac cacacgcaag tgtccataga
     1741 gatccccaac acgtgacaag ggtctgccct gcactttcag gatcttcttc tccaatgaca
     1801 gaccggattc aagccgaaca ggccctcttc tggtgtctat ctcaccctga atataggcca
     1861 catcctgccc gtgccgaatc agatatttct ctcgaagagt gcgatgcgaa gtggatgttg
     1921 ccagaaagag tatggattca aggatattgg tcttgccctg ggcgttggct ccgaggaaga
     1981 cgttgtaccc cgttccgagt ttgagatcgg cggcttcgat attgcgaaaa ttcctgacct
     2041 tgatttgatt cagaaacatg ttctttttga gcgccctgag ttcgggttga tttttcaccc
     2101 ctatgcctga taagattacc cgattctgta atcttcttca gggcggatca tcctgaccga
     2161 aacgggcgtc cggtgtcaac ccctgagaga gttgagtcaa tcgaggtcgg gaatgtgttc
     2221 ttctgacaaa gcgcaggttg ttgaggcagt ggtgctctcg gccaagaggc tcgaaggtcc
     2281 ctacctccga ctcgctgtcc gactgccgga gccaacactc tgttgtccgg gacagttctt
     2341 caatctgaaa acaggcggag agccagaacc cctttggcga aggcctttga gcgtcttcga
     2401 tcagaatgag gacactgtgg agttctatat acgcattgtt ggtcggggaa ccgaatggct
//
LOCUS       DAOQUC010000551         2455 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_52212_length_2455_cov_2.971667, whole genome shotgun sequence.
ACCESSION   DAOQUC010000551 DAOQUC010000000
VERSION     DAOQUC010000551.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2455
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52212_length_2455_cov_2.971667"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2455)
                     /locus_tag="PKH07_09955"
     CDS             complement(<1..>2455)
                     /locus_tag="PKH07_09955"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNT35306.1"
                     /translation="VKDEVSEKVNAITTFPTDAERPIVTELKLRRPVLQIALAGNLTE
                     RQIKELAEDIKKDIETLPHISQVSVGGTRDYEIAVEVSEERLREYGLTFGDVSRAIRR
                     GSMNLPGGTVRGENEEIKIRTLGRRYTGEEFASLVVLSKPDGTLIRLDQIASIRDEFE
                     EEGVYGTFNGKRAAIISVLKTEDEDAIAISKEVKEYIRRTKGILPPTVTLTPWADYSI
                     YVQQRLEMLLRNGRMGLILVFIILWMFLDIRLSFWVTMGIPISIAGALGLMALSGMTL
                     NMMTLFALIMIIGIIVDDAIVVGESIYIKRRQGLSPIQASLEGVAEVAWPITAAVTTT
                     LCAFLPLMFVGGVMGKFIKMLPQVVIWALSVSLFESLFLLPAHLSHLPDMNRPESELS
                     PLARRSFRIRRRVGTLLEWFTKRIYAPAITLASKFRYVTIALAIALLLLTFGMIAGGF
                     IKYTVFPKMDSDTMTARVEFPDGTPLQVTRQAVERIEAALMKLDRDFQKETGQPLVLS
                     RYAIAGSFTGAEGFSGSHLGEVSVEMLESKYRKWHYRDIVAVWEKEVGAIVGALSNTY
                     QGLQAGPGGKDIEIWLTGDRIEDLLAASSEIERNLRETAGVFEIENDFRPGKRELRAR
                     LKPGAEVLGITTDMLAEQLRSGYYGSEAVRIQRGRDDIRVKVRYPLAERRSLGDVEKI
                     RIRTATGQEVPLMAVADVSLEVGYTRIQRQNGKRRVIVTAETRPPANANEIMDFLQHS
                     LLPALTARYPGVRYSAEGSRQTTREALGSLKYGFPVAILGIFLILATEFRSYFQPIVI
                     MVTIPFGLIGAAFGHLLLGY"
BASE COUNT          559 a          712 c          626 g          558 t
ORIGIN      
        1 cataccccag aagaagatgc ccgaaggctg ctccgatcag accgaacgga atggtcacca
       61 tgatgacaat aggttggaaa tacgatcgaa attccgtcgc gaggatcagg aatatcccga
      121 gtatggctac agggaaaccg tatttgagtg atcccaacgc ctctcgagtg gtttgtcgag
      181 aaccttcggc agaataccgc actccgggat accgtgcagt caatgccgga aggagactgt
      241 gctggagaaa gtccatgatt tcgttggcat ttgcgggagg gcgcgtttcg gcagtgacga
      301 tcacccgtcg cttaccattc tgtctctgga tgcgcgtgta gcccacttcg agagagacgt
      361 ccgccactgc catcaaggga acttcctgtc ccgtagcggt tcggatcctt atcttctcga
      421 cgtcacccaa agagcgtctt tcagcgagcg gatagcgcac cttcacacga atgtcatctc
      481 tgccccgctg aatccgcact gcctcggatc cgtagtagcc cgagcgaagt tgttcggcaa
      541 gcatgtctgt ggtgatgcca agaacctcgg cacccggctt cagtcgcgct cgcagttccc
      601 gcttgccggg tctgaagtcg ttttcgattt cgaatacgcc tgccgtctcc cgaagatttc
      661 tttcgatctc cgaggatgcc gcgagcaaat cctcgatgcg gtctccagtg agccagattt
      721 cgatatcctt gccaccgggt cccgcctgca gtccctgata tgtattggag agcgctccga
      781 cgatcgctcc gacttccttc tcccaaacag cgacgatatc tctgtagtgc catttgcggt
      841 acttgctctc aagcatctcg acgctgacct cgcccagatg gcttcccgaa aaaccctcag
      901 caccagtgaa ggaacccgcg atggcatatc gagaaagcac cagaggttgc cccgtctctt
      961 tctgaaagtc ccgatccagt ttcatcaagg ccgcttcgat cctctccact gcctgccggg
     1021 tgacttgcag cggagtcccg tcgggaaact caacacgggc ggtcatggta tccgaatcca
     1081 tcttgggaaa gaccgtgtac ttgatgaaac cacctgcaat catgccaaac gtcaagagca
     1141 gcaatgcaat ggcgagagcg attgtcacgt aacggaattt tgaggcgagc gtgatcgccg
     1201 gtgcgtagat tcgcttggtg aaccactcca gcaacgtacc gacgcgtctt ctgatccgaa
     1261 aggatcttct cgccaaggga gagagttctg actcgggtcg gttcatatcg ggaaggtgac
     1321 ttaagtgtgc cggcaacaga aaaagactct cgaacaggga gacactcagt gcccagatga
     1381 ctacctgcgg aagcatcttg ataaactttc ccatcactcc acccacaaac atcaggggaa
     1441 gaaaggcaca gagtgttgtg gttaccgcgg cggtgatcgg ccaggctacc tcggcaacgc
     1501 cctccagcga ggcttgaatg ggtgagagtc cctggcgacg cttaatgtag atggattccc
     1561 caaccacaat ggcatcgtcc acgatgattc caatgatcat gatgagcgca aagagcgtca
     1621 tcatattcag tgtcataccc gagagagcca tcaaacctaa cgcaccggca atggagatgg
     1681 ggattcccat tgtgacccag aaactcagcc gaatatcaag aaacatccag agaatgatga
     1741 acacaagaat cagacccatt cggccgttgc gcaacaacat ttccagccgc tgctgaacat
     1801 agatcgaata gtcagcccac ggagtcagcg tcaccgtcgg gggaagaatc cccttcgtcc
     1861 ttctgatgta ttctttgact tctttggaga tagcgattgc gtcttcgtct tccgttttca
     1921 ggacgctgat gatcgccgcc cgtttgccat tgaaagtccc gtagacaccc tcttcctcaa
     1981 actcgtcccg aatgctggcg atctgatcca gacgaatgag cgttccgtcc ggtttcgaca
     2041 gaacaaccag actcgcaaac tcctcacccg tgtaacgcct gcccagcgtc cgaatcttga
     2101 tttcttcgtt ctctccgcga accgttccac cgggaagatt cattgagcca cgtcgaattg
     2161 cccgactgac gtctccaaac gtcagcccat actcacggag tctttcctcg gagacttcca
     2221 cggcgatctc gtagtccctg gtgccaccca cgctgacctg cgatatgtga ggcagggttt
     2281 cgatatcttt cttgatgtct tcggcgagtt ccttgatctg gcgctctgtc aagttgcctg
     2341 ccaaggcgat ttgaagcacc ggacgccgca gtttgagctc agtgacaatg ggtctctcgg
     2401 cgtcagtggg gaatgttgtg atcgcgttta ccttctcact gacctcatcc ttgac
//
LOCUS       DAOQUC010000552         2453 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_52277_length_2453_cov_3.874479, whole genome shotgun sequence.
ACCESSION   DAOQUC010000552 DAOQUC010000000
VERSION     DAOQUC010000552.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2453
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52277_length_2453_cov_3.874479"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2453
                     /locus_tag="PKH07_09960"
     CDS             <1..>2453
                     /locus_tag="PKH07_09960"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TaqI-like C-terminal specificity
                     domain-containing protein"
                     /protein_id="HNT35307.1"
                     /translation="FLKEIEGWRVELAKNIALRNTELSTDDLNRAVQLIIDRIVFLRM
                     AEDRGLEPYEQLLKLCSGADVYRKFAQKICRVADDKYNSGLFHFQKEPSVHEQPDRIT
                     PNLIIDDKVFKPILQKLYFEHGSPYQFNVLPVEMLGTVYERFLGKVIRLTKGHQAKVE
                     EKPEVRKAGGVYYTPSYIVDYIVKNTVGKQIEGKSPAQLSGKGKGDHIFRVLDMACGS
                     GSFLLGAYQYLLDHFLKWYLTNKPEKHPNAIRKATRSENARETTTPPHVSPDWCLTIQ
                     EKKRILTTHIFGVDIDPQAVEVSKLSLLLKVLEGESDESVGDTMRLWQERALPNLADN
                     IKCGNSLIAPDYFSGTLIPDPDEIKRVNPFDWKREFANAMSEGGFDCVIGNPPYVRQE
                     ILGELKTYLKEHYQVYHGIADLYSYFIERGVSLLCAGGIFSFIVANKWMRANYGEPLR
                     RWLKTKCVEEIIDFGDLPVFKNATTYPCIIRINNAEISAKPPSTIPCARIRTLDFRDL
                     TEHVQTCLFPVEISHLQDSGWTLADSTKQNLLAKIRAAGVPLGEYVKGKIYYGIKTGL
                     NEAFVIDRETRERLIAKDPKSAENIKPFLAGRDIKRYQPPTSEKYLICIPRGWTRQRS
                     EDTRNAWAWFEKNYPAIAHHLTPFAAKGEKRCDKGEYWWELRACDYYEEFEKPKIIFP
                     DMATRGQFTFDADGRFFSVNTTYFFTSSDVALLATLNSRLMAFYYAENYAAYRGGYLR
                     FFEQYLIELPIALSVVTSLPREKLSDLALRMLIFHRHFAAASSESERIVLQRQIDATD
                     REIDQLVYELYGLTEEEIR"
BASE COUNT          620 a          631 c          641 g          561 t
ORIGIN      
        1 gtttctcaag gaaatcgaag gctggcgggt ggaactggcg aagaatatcg cccttcggaa
       61 caccgagctt tccaccgatg atctcaaccg tgcggtgcag ttgatcattg accgcattgt
      121 ctttcttcga atggcggaag accgaggact agagccctat gagcaactgc tgaaactctg
      181 ctccggcgcg gatgtgtacc gcaagttcgc gcagaagatc tgtcgggtcg ctgatgacaa
      241 atacaattcc gggctcttcc actttcagaa ggagccgtca gttcatgaac aaccggatcg
      301 aatcacgccc aatctgatca ttgatgacaa agtcttcaag ccgatacttc agaaactcta
      361 tttcgaacac ggctcgccgt atcagttcaa cgtgcttccg gtggagatgc tgggcaccgt
      421 ctacgagcgt ttcctgggca aagtcattcg tctgaccaag gggcatcaag cgaaggtcga
      481 ggaaaagcct gaagtccgaa aagcaggtgg cgtctattac acaccgtcct acattgtgga
      541 ctacatcgtc aagaatacgg ttggcaaaca gatcgaaggc aagagtccgg cgcagttgtc
      601 aggaaaaggc aaaggggatc acatctttcg agttctggac atggcatgcg gttcagggtc
      661 gtttctcctg ggggcgtacc agtatctcct cgaccatttt ctcaagtggt acctgaccaa
      721 caaaccggaa aaacatccga acgcaattcg gaaagcaacc cgatccgaga acgcccggga
      781 aacaactaca ccccctcatg tctccccaga ttggtgcctg acgattcaag agaaaaagcg
      841 cattctgacg acgcacatct tcggcgtgga cattgacccg caggccgttg aggtctccaa
      901 actgtctctg ctgctcaaag tgcttgaggg cgagagcgac gaatccgtcg gggacacaat
      961 gcggctctgg caggagcgcg cgctgccgaa tcttgctgac aatatcaaat gcggcaactc
     1021 gctcatcgca ccggattact tctccggaac gctcattccc gatccggatg aaatcaagag
     1081 ggtgaatccg tttgactgga agcgggagtt tgcaaatgcg atgtccgaag gtggcttcga
     1141 ctgcgtgatc gggaatccgc cgtatgtccg tcaggaaatc ctcggcgaac tcaagacgta
     1201 tctcaaggag cactatcagg tctatcatgg cattgcggat ctgtattcct acttcattga
     1261 gcgcggcgtt tcacttcttt gtgcaggtgg tatcttctcg ttcatcgttg ccaacaagtg
     1321 gatgcgtgcc aactacggtg aaccgttgcg acggtggttg aagaccaagt gcgtggagga
     1381 gatcattgat ttcggggatc tgcccgtttt caaaaatgcc accacatatc cgtgcatcat
     1441 tcggatcaac aatgcggaga tctcggcgaa accgccttcg acaatccctt gtgcgcggat
     1501 tcgaacgctg gattttcgcg atctgactga gcacgttcag acgtgtctct ttccagtcga
     1561 aatctctcat ctgcaggatt ccggctggac acttgccgat agcacaaaac agaacctgct
     1621 ggcgaaaata cgcgctgccg gtgttccact tggggaatac gtgaagggga aaatctatta
     1681 cggtatcaaa accggtctca atgaagcttt cgtcatagac agagaaacgc gcgaacgcct
     1741 gatcgccaaa gatcccaaga gcgccgagaa catcaaaccg tttcttgccg gcagagacat
     1801 caaacgatat caaccaccaa caagtgagaa gtacttgatc tgtatcccaa gaggctggac
     1861 aaggcagcga tctgaagaca ccagaaacgc ttgggcttgg tttgagaaga actaccccgc
     1921 cattgcacac catctgactc catttgccgc caaaggagag aagcgctgcg acaaaggaga
     1981 gtattggtgg gaactcaggg catgtgacta ctacgaggaa ttcgagaagc cgaagattat
     2041 cttccctgat atggcaaccc gcggacagtt cacatttgat gccgatggca gattcttctc
     2101 ggtgaatacc acctatttct tcacttcctc agacgttgct ctcctggcaa cactgaactc
     2161 aagattgatg gcattctatt atgctgagaa ctatgcagcg taccggggcg gctatctccg
     2221 cttctttgaa cagtatctca tcgaactacc tatcgctctt tcagtggtta cttcgttacc
     2281 aagagagaaa ctgagcgatc ttgcacttcg aatgctcatc ttccaccggc attttgcggc
     2341 agcgtcttct gagtcggagc ggatagtctt gcagcgtcag attgatgcca cggatcggga
     2401 gatcgatcag ttggtgtatg agttgtacgg gttgacggaa gaggagattc gga
//
LOCUS       DAOQUC010000553         2450 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_52386_length_2450_cov_2.933612, whole genome shotgun sequence.
ACCESSION   DAOQUC010000553 DAOQUC010000000
VERSION     DAOQUC010000553.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2450
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52386_length_2450_cov_2.933612"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..573
                     /locus_tag="PKH07_09965"
     CDS             <1..573
                     /locus_tag="PKH07_09965"
                     /inference="COORDINATES: protein motif:HMM:NF014017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HNT35308.1"
                     /translation="QEATLKKWLEATDEGFLFSMQANRLITHSRKLRDVDGLLHAFLD
                     RISTMGSQLAPTLFQLPHSMEKDTALLRDFLALLPGDRESVMEFRHASWFDDEVLMCL
                     REHRVGYCIVSAPRLPVRIEVTAPFAYIRLHGKRRWFTDNYTDEDLEWWAREVRHILD
                     QGTRVFAYFANDEDAAAVFNAKKFEQLLSP"
     gene            complement(604..>2450)
                     /gene="dxs"
                     /locus_tag="PKH07_09970"
     CDS             complement(604..>2450)
                     /gene="dxs"
                     /locus_tag="PKH07_09970"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HNT35309.1"
                     /translation="SDLDMLAEEIRDLIVTTVSRTGGHLAPSLGAVELTLALHRVFDS
                     PRDKIIWDVGHQSYAHKILTGRRDQFHTLRQFKGISGFPRIEENPHDIFGAGHSSTSI
                     SVAVGLAAARDFREEKHKVIAVIGDGALTGGMAFEALNHAGSLNMDLIVILNDNEMSI
                     SPNVGGMSKYLSRIITGQFYNQAKVDIEDFVNRLPRIGPKLKRLLNRMEESLKGFIVP
                     GVLFEELGFRYFGPIDGHNMPLLVETFSNLQRIGGPVLLHVITRKGKGYVPAEEHPTH
                     FHGTGPFDKVSGEPKKSSKDTYTDVFGRVVTRLAATDRRVVVITPAMTVGSGLVEYAQ
                     QFPDRFFDVGIAEQHATTLAGGLARGGMKPILAIYSTFLQRAYDQVFHDVCLQNQPVV
                     FAIDRAGLVGPDGATHQGVFDISYLRVLPNLTVMAPMDEVELEMMLSHSLSINSPVAI
                     RYPRDYVEQVTESQLHHQPLQEGKALVVRDGKDVAIWAVGPLVYRVLDALPILEKAGV
                     RPVVVNPRYLKPVDDELICRQARKGFRIVTFEEHALAGGFGSVLLETLEQRGIADTPV
                     LRLGIPDCFVEHGTRDQLLKLLQLDTQSIAERIIRFVNEPVSISARQG"
BASE COUNT          598 a          701 c          623 g          528 t
ORIGIN      
        1 caagaggcca cgctcaaaaa atggcttgag gccaccgatg agggatttct cttttccatg
       61 caagccaaca gactcatcac acactccaga aaacttcggg atgtggatgg acttcttcac
      121 gcattccttg atcgaatctc gacaatgggc agtcagctcg ccccaaccct gtttcaactg
      181 cctcactcga tggagaaaga taccgcgctc ttgcgggact tcctggcgtt gcttcccgga
      241 gacagggaat ccgtcatgga attcagacac gccagttggt ttgatgatga agttctcatg
      301 tgccttcgtg agcatcgagt tggctactgt atcgtcagtg cgccaagatt gccagtgcgc
      361 attgaagtaa ccgcaccctt cgcgtacatc cgacttcacg gcaagcgccg ttggttcaca
      421 gacaactaca ccgacgagga cttggagtgg tgggcgaggg aagtccggca catactcgat
      481 caagggacaa gagttttcgc atacttcgcc aacgatgaag atgccgccgc ggtgttcaac
      541 gccaagaaat tcgagcagct gttgtcgccg taaggaagat cccccgaaca aacggtgtga
      601 ttctcagccc tgacgtgccg agatggaaac aggctcgttc acaaaccgga tgatccgttc
      661 tgctattgac tgggtatcca actgaagcag tttcaagagt tgatccctgg tgccgtgttc
      721 aacaaaacag tctggtattc caagtcgcaa cacaggtgta tcagcaatcc cacgctgctc
      781 caaggtctcc aggagtacag agccaaaacc acccgcaagc gcatgttcct caaacgtcac
      841 aattcggaac ccctttcggg cttggcgaca gatgagctcg tcatcaaccg gcttcagata
      901 gcgcgggttg accaccactg gacgaactcc tgctttctcc aaaatgggaa gcgcgtcaag
      961 cacccggtaa accaaggggc ctaccgccca aatcgccaca tcttttccat cacgaacaac
     1021 aagggctttg ccctcctgca gtggttggtg atgcagctgg gactcagtca cttgctcgac
     1081 atagtcccgc gggtaccgga tggcaacagg actgttaatc gagagcgagt gggaaagcat
     1141 catctccaac tcgacctcat ccattggtgc catcacggtc aaattgggaa gcacccgcag
     1201 atagctgatg tcgaaaaccc cttgatgtgt cgccccatca gggccaacca gtcccgcacg
     1261 gtctatcgca aacacgacgg gctgattctg aaggcagacg tcatggaaaa cttgatcgta
     1321 cgctcgctga agaaacgtcg aataaatcgc cagaatcggc ttcatgcctc cacgcgccag
     1381 accaccggca agcgttgtag catgttgctc ggcgattcca acatcgaaga agcggtcggg
     1441 aaactgctga gcatattcga ccagaccact gccaacggtc atcgccggcg tgatcaccac
     1501 aacgcggcga tctgtagctg ccagtcttgt gacaacgcga ccaaagacat ccgtgtaggt
     1561 atccttggat gacttcttcg gttcacccga aaccttatcg aatgggccgg tgccgtgaaa
     1621 gtgcgttgga tgctcttcgg caggcacata gcctttcccc tttcgggtga taacgtgaag
     1681 aagaacaggc ccaccaattc tctgaagatt gctgaatgtt tcgacgagca aaggcatgtt
     1741 gtggccatct atcgggccga aataacgaaa gccaagttcc tcaaaaagca ccccgggaac
     1801 aatgaaacct ttgaggcttt cctccatccg attcagaagt cgtttcagct tcggaccgat
     1861 ccttggaagc cgattcacaa agtcctcaat gtccaccttt gcctggttgt agaactgacc
     1921 cgtgatgatg cggctgaggt acttcgacat acccccaacg ttgggggaga tggacatctc
     1981 gttgtcattg aggatcacga ttaagtccat gtttagactg cccgcgtgat tcagtgcctc
     2041 aaatgccata ccccccgtca aggcaccgtc cccgatgacc gcaatgacct tgtgtttttc
     2101 ctcacggaag tctcgagccg cagccagacc gaccgccacg gaaatggaag tgctgctgtg
     2161 acccgctccg aagatgtcat gcggattctc ttcgatacgg ggaaagccac tgattccttt
     2221 gaattggcga agtgtgtgga attgatcccg ccgacccgtc aggatcttgt gcgcatacga
     2281 ctgatgaccc acgtcccaga taatcttgtc gcgcggactg tcgaatacgc ggtgaagggc
     2341 aagtgtcagt tcgacagcac ccagactcgg agccaggtgc ccccccgtgc gggacaccgt
     2401 cgtcacgatc agatcccgaa tctcttctgc aagcatatcc agatcactgt
//
LOCUS       DAOQUC010000554         2449 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_52415_length_2449_cov_3.131161, whole genome shotgun sequence.
ACCESSION   DAOQUC010000554 DAOQUC010000000
VERSION     DAOQUC010000554.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2449
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52415_length_2449_cov_3.131161"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(93..1505)
                     /gene="hslU"
                     /locus_tag="PKH07_09975"
     CDS             complement(93..1505)
                     /gene="hslU"
                     /locus_tag="PKH07_09975"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014098676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HNT35310.1"
                     /translation="MTKEPGVQQPQYRIDDLTPREIVTELDKYVVGQDDAKKAVAIAL
                     RNRARRRELSAELQEEIYPKNILMIGATGVGKSEIARRLARISNAPFYKVEASKFTEV
                     GYVGRDVESMIRDLVEIAVNDVREYQMEECMEMAESLAEERLLDLLLPAQHPSEVEAH
                     EGAEVSSESHDAMVARLHETREKLRKKLRNGDFEDRVVEPEVRERTTSVMGFIPGGPG
                     MEEIGIDLQEMLERMMPSRKKRKRMTVAEARPFLEQEEASRLIDMDAVVREALDRVEE
                     LGIVFLDEIDKIVGGEARSGPDVSREGVQRDLLPLVEGTAVFTKYGVVRTDHILFIAA
                     GAFHASKPSDLIPELQGRFPIRVELQSLTQRDFERILTEPKNSLLIQYQALMETEGIK
                     LEFKENAVTAIASLACQVNRETENIGARRLHTMVEKLLEELSFNAPDMDEKHFVIDEI
                     YVQERLSVLVTDSDLSRYIL"
     gene            1633..2265
                     /locus_tag="PKH07_09980"
     CDS             1633..2265
                     /locus_tag="PKH07_09980"
                     /inference="COORDINATES: protein motif:HMM:TIGR01470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HNT35311.1"
                     /translation="MYTIALVLQGRRCVVIGGGQVAQRKILSLLESEAEVLVVSEQLT
                     TTIREWVDQQRVQWLARRFQSGDLNGAFLAIAATNDRTVNEAVAKEAARERVLINVVD
                     DPELCDFYVPAVLKRGDLQIAVSSGGKSPVLSKHVRERLEKQFGEEYGLFLNMVKRLR
                     EQMRLLRLDRAIRHEAEKSFVESPALSLLAQGKREQAEKVLRECLSKYTD"
     gene            2241..>2449
                     /locus_tag="PKH07_09985"
     CDS             2241..>2449
                     /locus_tag="PKH07_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HNT35312.1"
                     /translation="MFVEVHGLNHTTASVEIREKVWIPPDRLAEALVMLKSLPHVEQC
                     FILSTCNRTEVYVLRPTSPGATGRPA"
BASE COUNT          530 a          680 c          656 g          583 t
ORIGIN      
        1 ctggaactac ttcgatctca ccgaactgat aggtttgaat cttcacgatt ccctgtccct
       61 ttttgccgtt cagtgaacgc cgaacgggat tcctaaagaa tgtaccgact caggtcggaa
      121 tcggtcacca agaccgatag tctctcttga acgtagattt cgtcaatgac gaaatgcttt
      181 tcatccatgt cgggagcgtt gaacgacagt tcctcaagga gtttctccac catagtatgc
      241 agacgccgcg ctccaatgtt ctcggtctca cgattgacct gacaagccag cgacgcgatg
      301 gcagtaacgg cattctcctt aaattccagt ttgatacctt cggtttccat gagcgcttga
      361 tactggatga gcagggagtt cttgggttct gtcaggatgc gctcgaaatc ccgctgtgtc
      421 agggattgca gttccacgcg aatgggaaag cgtccctgga gttcagggat caaatccgat
      481 ggttttgagg cgtgaaaagc tccggcagcg atgaacagga tgtggtcggt tctcacgaca
      541 ccgtacttag tgaagaccgc ggttccctca accaggggga gcaagtctct ctggactcct
      601 tcacgggaaa cgtcagggcc ggaacgggct tctccgccca cgatcttgtc gatctcgtcc
      661 agaaagacga tgccgagttc ctcgactcga tccaaggcct cccggacgac ggcatccatg
      721 tcgatcaagc ggctcgcttc ttcctgttcc aggaaaggcc gtgcttcggc aaccgtcatg
      781 cgcttgcgct tctttcggga cggcatcatc cgctccagca tttcctgaag gtcaatgccg
      841 atctcttcca tacctggccc gccggggata aatcccatga cgctggtggt tcgctcacgg
      901 acttctggct cgacaacacg atcctcaaaa tccccattgc ggagtttctt gcgaagtttc
      961 tcccgcgttt cgtgcagccg tgctaccatg gcatcatggc tctcggaact cacctcagca
     1021 ccttcgtgcg cctcgacttc cgatgggtgc tgggcgggaa gaagcagatc caggagccgc
     1081 tcttccgcga gggactccgc catctccatg cactcttcca tctggtattc acgaacatcg
     1141 ttgacggcga tctcaaccag atccctgatc atggactcca catcacggcc gacatatccg
     1201 acctcagtga acttggatgc ctcgaccttg tagaagggtg cgttggaaat gcgggccagg
     1261 cgacgggcga tctcactttt tcccacgccg gtggcaccaa tcatgaggat gttcttgggg
     1321 tagatttcct cctgaagttc agccgacaat tcccgtcgtc gagccctgtt tcgaagggca
     1381 atcgccacgg ctttcttggc gtcatcctgc ccgacaacat acttatccaa ctcggtgaca
     1441 atctcccgcg gtgtcagatc gtcaatacgg tactggggtt gctgcacccc gggttctttt
     1501 gtcaatgaat catccttgtc catctttggc ctttccggcg gtatcaatca tttctcatga
     1561 ccgcacgttt catacccatc tgacttgccc aatgcaagtt caagcatgta tcatacatga
     1621 gaagaggtaa atttgtatac tatcgctttg gtgctacaag gacgacgttg cgttgtcatc
     1681 ggaggcggcc aggtcgccca acgcaagatt ctctctttgc tggaatcgga agccgaagtc
     1741 ctcgtcgtca gcgagcaact gacaacaacg attcgagaat gggtggatca gcagcgagtg
     1801 caatggcttg cccgacgatt tcagtcggga gatttgaatg gagcgtttct ggcaatcgcc
     1861 gcaaccaacg atcgaaccgt gaacgaggct gttgccaaag aagccgcgcg cgagagggtg
     1921 ctgattaacg tagtggacga tcctgagtta tgtgatttct acgttccagc agtgctcaaa
     1981 cgcggagacc ttcagatcgc cgtctcgtcg ggtggcaaga gtcctgtcct gtccaaacat
     2041 gttcgggaac gccttgagaa acagtttggt gaggagtatg gtctctttct gaatatggtg
     2101 aagaggctcc gagaacagat gaggttactc cgtctggaca gggcaattcg tcacgaagcg
     2161 gagaagagtt ttgttgagtc tccggcgctc tccctgctcg cgcaagggaa aagagaacaa
     2221 gccgaaaagg tcttgaggga atgtttgtcg aagtacacgg actgaaccat acaactgcaa
     2281 gcgttgagat ccgggagaag gtctggattc ctcccgatcg ccttgccgag gcgctggtga
     2341 tgctcaagag tctcccgcac gtcgagcagt gtttcattct ttccacgtgc aacagaacgg
     2401 aagtctacgt cctgcgcccc acgtcccccg gggcaacggg gcggccggc
//
LOCUS       DAOQUC010000555         2448 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_52451_length_2448_cov_3.198913, whole genome shotgun sequence.
ACCESSION   DAOQUC010000555 DAOQUC010000000
VERSION     DAOQUC010000555.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2448)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2448)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2448
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52451_length_2448_cov_3.198913"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..672)
                     /locus_tag="PKH07_09990"
     CDS             complement(<1..672)
                     /locus_tag="PKH07_09990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35313.1"
                     /translation="MHRRLLLVLALCSIVPMAMADTVIESFENANPAGWRTTSPQPGQ
                     DRNIASPRYSPIPQYVTEHVTHGAAAGRFGANWTLPGVDYPANANVYESGGTTLYWAL
                     RISTASGAALGNIPTSAELHADIYNRTADPMQFAFVVSDGGLERGPLTTLAPNASTEY
                     VWKLSEGCTGWVTGNGVWNSGTVVMNSMLLYTTTAPTAAQFTFDIDNVRVVTPQTDFT
                     PPAPPV"
     gene            complement(1131..2261)
                     /locus_tag="PKH07_09995"
     CDS             complement(1131..2261)
                     /locus_tag="PKH07_09995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35314.1"
                     /translation="MKRIILAAVLMLVVSPAFGQLAGISVDWAKTMTMEGAVSSVAYN
                     YATDHFLVCQYNAPVGKKVAIVNQAGDTILGYLNETGLTFGTLNVFAICVGSDGVIFG
                     GGNPDPDGAGTLEDRYLYRWANESAVPTQFNLGQADSANAANDFGMIFPRAMDVYGTG
                     VNTVVASSGDNSYDVSILTTTDGVNFTFAYRTNADNATDAATQIKQGVALTSDPAEIY
                     GVKADGAGQVVSIVKSVTDTWFAKSGFVPANSYGVPPAGFGAAALIDYSEAQNAVFVI
                     GYSQTDDWLTALDGTTGATLFQTQIGQNVSTYGYGTMSVNDAQLGGYFGSRALGTNLA
                     LFGKFSYTTLATPTNTPTETPTFTPEATPTKSSGVKEFEVYE"
BASE COUNT          649 a          670 c          577 g          552 t
ORIGIN      
        1 caccggagga gctggaggcg taaaatcggt ctgaggcgtc acgactctga cgttgtcaat
       61 atcaaaagtg aactgcgctg ccgttggtgc cgtggttgta tacagaagca ttgagttcat
      121 gaccacggtg cctgagttcc agacaccgtt tccggtgacc caaccggtgc atccctcaga
      181 aagcttccag acatattctg ttgatgcgtt cggcgccaat gtcgtgaggg gacccctctc
      241 caaaccaccg tcactgacca caaaggcaaa ctgcattgga tcagcagttc ggttgtagat
      301 gtccgcatgc aattcggctg atgtaggtat gttccccaaa gcggctccac tggctgtgct
      361 gatgcggagc gcccagtaga gggttgtccc gccactttca tagacgttcg cgttcgccgg
      421 atagtcaaca cctggcagag tccagtttgc accaaagcgt cccgcggccg caccgtgggt
      481 gacatgctcg gtaacgtatt gcggaattgg cgaataccga ggtgacgcaa tgtttctgtc
      541 ctgacccggc tgaggcgatg ttgtccgcca ccctgccgga ttggcattct caaaactttc
      601 gataaccgtg tctgccatcg ccatgggaac aattgaacaa agagccaaaa ccagcaaaag
      661 tcttctatgc atctctcaaa actcctttca cgaaaaacaa gacgaaaaaa agtataacac
      721 tcgctcaaaa accaaatgat caactcttcc gagaactacg ctgcaatcac ctccttgcct
      781 tcggcaaccc gatacataca tacccacttc gttaaggcca aagatatcat ccctgaaaat
      841 gacgtgtcaa tgagttttct cagaaaatgg tactgcctca gctatcgcag gggggcacga
      901 agaacgacag tgtaagcaag atgtcactct gagcggagtc ttcggagcga agagtctctc
      961 tttgccttcg ccgaagggct tcggactgta ggagagattc ttcacttcgc tcccaagagc
     1021 gttgctacgt tcagaatgac atacccttct ttcgggagag catccccaaa gaaacagggc
     1081 tgggaacaat cccagccctg tttgtttgtt catcgaatcc gaagtaacga ttattcgtag
     1141 acttcaaatt ccttcacacc ggaggacttt gtcggtgtcg cttccggagt aaatgtcgga
     1201 gtctcggtcg gagtgttcgt tggcgttgcc aatgtggtgt aggagaactt gccaaagagc
     1261 gccaagttcg tgccgagtgc acgtgatccg aagtaaccgc caagttgagc atcattgacg
     1321 gacattgtgc cgtagccgta agtgctgacg ttctgtccaa tctgagtctg gaacagagtt
     1381 gcacctgttg tcccatcaag cgcggtcaac cagtcgtcgg tctggctgta accaatgaca
     1441 aacactgcgt tctgtgcctc tgaatagtca atgagcgccg cagcgccaaa acccgccgga
     1501 ggaacaccgt agctgtttgc cggaacaaaa ccacttttcg caaaccaagt gtctgtcact
     1561 gacttgacaa tcgagacaac ctgacccgcg ccatcagctt tgacgccgta gatctctgcg
     1621 ggatcacttg tcagagcaac gccctgctta atctgggtgg cagcatcagt cgcattgtca
     1681 gcgttggttc tgtatgcgaa cgtgaagttg actccatcgg tcgtcgtcag gatggaaacg
     1741 tcgtaagaat tgtcacccga tgaagcaaca acggtgttca caccggtgcc ataaacatcc
     1801 atggcacggg ggaaaatcat cccgaaatcg ttagctgcat tcgctgagtc agcttgacca
     1861 aggttgaact gagttgggac tgccgactca ttcgcccaac gatagagata ccgatcctcg
     1921 agtgtaccgg caccatcagg atctgggttg ccaccaccaa agatcactcc gtctgaacca
     1981 acgcaaattg caaagacatt aagagtccca aaagtcagac ccgtctcatt taagtaacca
     2041 agaatcgtgt cacccgcttg attgacgatg gcaaccttct tgcccacagg ggcgttgtat
     2101 tggcagacca gaaaatgatc cgtcgcatag ttgtaagcaa cggaagaaac cgcaccttcc
     2161 atcgtcatcg tcttagccca atctaccgag ataccggcaa gttgtccaaa cgccggagaa
     2221 acaaccaaca tcagaactgc agccaaaata attctcttca tttcagaaaa ctcctttcaa
     2281 aatagaaaac accaaaataa aacaaaacac acgtcgccag ataaaatcac cgatttgtcc
     2341 tttcgcctag accatcacct cctcattcag cagcgccgga acagtctctc cggcaatgct
     2401 gctcagaaac caaatcaact tcctgaacta acctatatca ctttccga
//
LOCUS       DAOQUC010000556         2442 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_52666_length_2442_cov_2.181818, whole genome shotgun sequence.
ACCESSION   DAOQUC010000556 DAOQUC010000000
VERSION     DAOQUC010000556.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2442
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52666_length_2442_cov_2.181818"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..156)
                     /locus_tag="PKH07_10000"
     CDS             complement(<1..156)
                     /locus_tag="PKH07_10000"
                     /inference="COORDINATES: protein motif:HMM:NF013707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HNT35315.1"
                     /translation="MEQRIEVGGHGGQGVVLMSYLLAHLGMRHGLNVTWFPSYGAEMR
                     GGTASCSV"
     gene            complement(141..885)
                     /locus_tag="PKH07_10005"
                     /pseudo
     CDS             complement(141..885)
                     /locus_tag="PKH07_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739363.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
     gene            complement(863..>1805)
                     /gene="vorB"
                     /locus_tag="PKH07_10010"
     CDS             complement(863..>1805)
                     /gene="vorB"
                     /locus_tag="PKH07_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HNT35316.1"
                     /translation="AAGGAFVQAESELAAINMVLGAAAAGARAMTSSSSPGISLMQEG
                     ISYMAGQRLPAVIANVVRGGPGLGNISAAQSDYFQSTRGGGHGDYYTPVLAPNSVQEM
                     YDLMYDAFAIAERYRTPTIVLADGMLGQMAEPLAIRSRPAPPAIEKPWALTGAKGRAP
                     NSVFSLMIGPGRLEEHVRTLKNTYREMEREIRFERFQCDDAETIMVAYGSCARFCRKA
                     VVLARSKGVRVGLFRPITLFPFPAEALQKEIARGTRDFLVAELSAGQMVEDVKLSLWG
                     SNATVRFFGRTAGSFPSTDSILAALRGEPQDGSFYEA"
     assembly_gap    1806..1815
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1816..1933)
                     /locus_tag="PKH07_10015"
     CDS             complement(<1816..1933)
                     /locus_tag="PKH07_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007935260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     beta"
                     /protein_id="HNT35317.1"
                     /translation="MIGNDALCQGAIDAGCRFYAGYPITPQNETIEYMANHME"
     gene            complement(2012..>2442)
                     /locus_tag="PKH07_10020"
     CDS             complement(2012..>2442)
                     /locus_tag="PKH07_10020"
                     /inference="COORDINATES: protein motif:HMM:NF013887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35318.1"
                     /translation="CTESLRAFQRRRPPHAATHDTQYTSPGHTIGHALETCTEYHEWL
                     HGEAVAVGMLAETRIAQKLGLTTDDTFRRQEQLFQKVGLPTRLPSMDMERFQKTVLHD
                     KKNLQMKIRLVLPVRIGQVQVVDDVPLSLVSETVRELTSQ"
BASE COUNT          539 a          728 c          628 g          537 t
ORIGIN      
        1 cacggaacag gacgccgtac cacctcgcat ctctgcacca taggagggga accacgtgac
       61 attcaaaccg tgacgcattc ccagatgcgc caacaagtac gacatcagga caacaccctg
      121 tccgccatgt ccaccaactt ctatccgttg ttccattgcg gctccttgta gacacccaac
      181 gggaattcga ctttgttgac ttcattgatg tacttcatgg attcgaccgg tgtcattccc
      241 caattgggtg gacaatttga aagaatctca acaagcgaaa aaccaaggcc tgcaatctgt
      301 acctgaaagg acttgactat ggctttcttg gctttgatga catccttggg tgagtcaatc
      361 gcgacgcgct caagatagac aaccccagga agcggggcga gcatttcgca caccttgagt
      421 ggaaaccctg cacccgctgc attgcgtccc aaaggagttg tcttggttct ttgtcccatg
      481 agcgttgtgg gtgccatctg accacccgtc attccataga ccccgttgtt catgaagacg
      541 acggaaatcc gttctccccg cgcggcgctg tggataatct ctccggtgcc gatggcggcc
      601 aagtctccgt caccctggta cgtgaacacg gtatggtcgg gcagtacacg cttgatgccg
      661 gtggcgacgg caggagtcct gccatgaggc gcttccgcca catcaaagtt ccagtaatca
      721 taggagaaaa cggcacaacc cactggagcg actccgatcg tcgtctctga ttcccaactg
      781 gtctatagct tctgccacca atcgggtagc gatgccatgt ccgcagcccg ggcagtagcg
      841 ggtctgctca gatgtcattg cctcaggctt cgtaaaaact accatcttgt ggttctcctc
      901 tcagtgcggc gaggatgctg tcagtactcg ggaaagaacc tgcagtgcgt ccgaagaatc
      961 gcactgttgc attggaaccc caaagtgaca gtttgacatc ctcaaccatc tgtccggcac
     1021 tcagctcagc gacgaggaaa tcccgtgtgc cacgggcaat ctctttctga agggcttcag
     1081 caggaaaggg gaatagtgta atcggacgga aaagcccgac ccgaacgccc ttcgagcgag
     1141 caagcactac tgcttttcga cagaatcggg cacacgatcc ataagcgacc atgatcgtct
     1201 cggcgtcatc gcactgaaaa cgctcaaaac gaatctcgcg ctccatctca cgataggtgt
     1261 tcttcagagt tcgaacgtgc tcctccagtc gccccggccc gatcatcagg gaaaacactg
     1321 agttgggcgc gcgtcctttt gccccagtca gcgcccaagg cttttcgatc gcaggtggcg
     1381 ccggccggct cctgatcgca agtggttcgg ccatctgccc caacataccg tctgccagaa
     1441 caatggtagg cgttcggtag cgttctgcaa tggcgaaagc gtcgtacatc agatcataca
     1501 tctcctgcac actattggga gcgagtactg gcgtgtaata gtctccatga ccgccaccgc
     1561 gtgtggactg aaaatagtct gactgcgccg cggagatatt ccccagtcca ggtccccctc
     1621 gcacgacgtt ggcaatgacc gcgggcaaac gttgacccgc catataggaa atgccctcct
     1681 gcatcaggga aatcccaggg ctggaggaac tcgtcatggc gcgggcacct gcggccgccg
     1741 cgcccaacac catgttgatc gcggccagtt cactctccgc ctgcacaaag gccccaccgg
     1801 cagccnnnnn nnnnncctcc atgtggttgg ccatatactc aattgtttca ttttgcgggg
     1861 tgatcggata gccggcgtaa aaccgacatc cagcatctat ggctccctgg caaagggcat
     1921 catttccgat catcagaacc ttgccattgg ttgtcacttt gaagcctctt ttcaaaggcg
     1981 ctcttgagtc gagcaccgcg ttttatcgtt cctactgact ggttagttcc ctgaccgttt
     2041 ccgagaccag cgacaatggc acatcgtcaa ccacctgaac ctgcccaata cggacgggca
     2101 gaaccagtct tatcttcatc tgaaggttct ttttgtcgtg cagcaccgtc ttctgaaacc
     2161 gttccatgtc catggagggc aggcgcgtcg ggagaccaac cttttggaac agctgttcct
     2221 gtctgcggaa ggtatcatcc gtcgtcagac cgagtttctg ggcgatacga gtctctgcga
     2281 gcattccaac ggcgaccgcc tccccatgca gccactcatg atactcagta cacgtctcca
     2341 gggcgtgccc aatcgtatgc cctggagacg tgtactgagt atcatgagtg gctgcatggg
     2401 gaggccgtcg ccgttggaat gctcgcagag actcagtaca cg
//
LOCUS       DAOQUC010000557         2438 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_52804_length_2438_cov_2.824171, whole genome shotgun sequence.
ACCESSION   DAOQUC010000557 DAOQUC010000000
VERSION     DAOQUC010000557.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2438
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52804_length_2438_cov_2.824171"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..473
                     /locus_tag="PKH07_10025"
     CDS             <1..473
                     /locus_tag="PKH07_10025"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNT35319.1"
                     /translation="LLGYEITMMSMFGMVALAGIVVNDAIVFIDCFNERLRQGLPFIE
                     ALREGGMRRLQPILLTTSTTVAGLAPLIMEKSFQAQFLIPMAVSIASGLIFATLLTLF
                     FIPCLLVALNDLRRLWRLLWTGSMPTREAVEPAITLRVEDHEPFVESKVRDYGV"
     gene            463..1941
                     /locus_tag="PKH07_10030"
     CDS             463..1941
                     /locus_tag="PKH07_10030"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNT35320.1"
                     /translation="MEFRRGIANLFRVTDVTLIRRKATRRSARRPMWRLAPVFLCVCS
                     LLSFGCAVVGTGVYDVASMTLSPLPELSESQELILSEILTLDQAKQIALENSPTVQQA
                     LARISAAEARIMEARSAYFPKLDAEGAAIRTHQSPDWQTVGSERRTLYSNELSVSWVL
                     FDGLARRARLQSARYGKLSNLAARENVCRLLLRGVVESYMGAQLADARIQIASSDAEF
                     NTVRLDESKKKERAGSASRSEVLNFEIRVNQALSDVVTARVSRELALATLADLLGVPA
                     TGFCEVVRLEPLPSESSAVPPISIEEEVEQALQNRPDLLVQRSALEQSRLDVKASRAE
                     FLPKVVLQGSTGHQSIEDFSTSEEDRATSYGVFAQWNLFSGGARLARLRATQARTKEQ
                     EQLVEEMTLGIVAETRQVCSQILAASEQVALTRHSLDLTEATRNLVEKEWQAGQASLT
                     RLNEAQSDLIRAQSRFALTLIQQRQALENLRVVTAKDTSRDW"
BASE COUNT          543 a          642 c          722 g          531 t
ORIGIN      
        1 ttcttctggg gtatgagatc accatgatga gtatgtttgg catggtggct ctcgcgggaa
       61 tcgtcgtcaa tgacgcaatt gtgttcattg actgtttcaa tgagcggttg cgtcagggct
      121 tgccctttat cgaggcgctg cgcgaaggcg ggatgcggcg actgcagccg attcttctca
      181 ccacatccac cacggttgcg ggcttggcgc ctctcatcat ggagaagagt tttcaagcac
      241 agtttctcat cccgatggcg gtgtcaatag cttcgggact catttttgca acgctcttga
      301 ccctgttctt catcccgtgt ctgctggtgg cgctcaatga tctccgccgc ttgtggagac
      361 tgctctggac aggaagtatg ccgacgagag aggcggtcga accagcgatt acactgcggg
      421 tggaagacca tgaacctttc gtggaatcta aggtgaggga ctatggagtt taggagagga
      481 attgccaatc tgtttagagt gacggatgtg acactcatca ggcgaaaagc gacacgaaga
      541 tctgcacggc gtcccatgtg gagacttgct cccgtctttc tgtgtgtttg ttctctgttg
      601 tctttcggat gcgccgtagt gggcacaggc gtctacgatg tcgcgagcat gacgctttcc
      661 ccgttgcccg aattatcgga atctcaggaa ttgatccttt cggaaatcct tacactggat
      721 caggccaagc agatcgcttt ggagaacagc ccgacagtcc agcaggcact cgcgcgcatc
      781 agcgccgcag aggccaggat catggaagcg cgttctgcct attttccgaa actggatgcc
      841 gagggagcgg ccatcagaac ccaccaatca cccgattggc agacggtcgg ttccgaacgc
      901 cgaacgcttt attccaacga actcagtgtc agttgggtct tgttcgacgg actggctcgg
      961 cgtgcacgtc ttcagtctgc ccgatatggg aaactatcca acctggcggc tcgcgaaaac
     1021 gtctgccgac ttctgctgcg cggcgtggtg gaatcgtaca tgggcgcgca actggcagac
     1081 gcacgaatcc agatcgcctc ttcagatgcc gagttcaaca cggttcgact ggatgagtcc
     1141 aagaagaagg aacgtgcagg aagcgcttct cgaagcgaag tgctgaactt cgagattcgt
     1201 gtcaatcagg ctttgagcga tgtggtgact gctcgcgtgt ccagagaact ggcgcttgct
     1261 acgctggctg atcttctcgg agtgcccgcc acgggtttct gtgaagtggt acggctcgaa
     1321 ccgcttcctt cggagagcag cgccgtccca ccaatcagca ttgaagaaga agtcgaacag
     1381 gcgcttcaga atcggcctga ccttctcgtt cagcgatcgg cattggagca gtcccggctc
     1441 gatgtcaaag catcccgtgc ggagtttctg ccaaaagttg tgcttcaagg cagcaccgga
     1501 catcaaagca tcgaagactt cagcacgtcc gaagaagacc gcgccaccag ctacggagtc
     1561 ttcgcgcagt ggaatctctt ctcaggggga gcacgactgg ctcgacttcg tgccactcag
     1621 gcacgaacga aggaacagga gcagttggtc gaggaaatga ccttgggaat tgtcgcagaa
     1681 acaaggcagg tatgttccca gattcttgcc gcatccgaac aggtcgcgtt gaccaggcac
     1741 agcctcgatc tgacggaggc aactcgaaac ctggtcgaga aggaatggca ggcgggacag
     1801 gcgtctctga cgagactcaa cgaagcacaa agtgatctca tccgcgccca gagccgcttc
     1861 gcgcttaccc tcatccaaca gcgtcaggca ctggaaaacc ttcgtgttgt gacagccaaa
     1921 gacacttcac gggattggta gaaggtctcc agcacctatt cccaaaaacg tcttgagaca
     1981 gtcttctcat tgagggctgc tctgggatcg ccgcgcgtct gcttgggaat ctggttgtgg
     2041 cgcaggcatc ttgcctgcac aaaggagcgg gagcatcttg ctcccgacga aatccgtgtg
     2101 ggcgagacgc tccagctcgt gaagaatttg accgtgtgcc tcaaatatga gagacttgac
     2161 atcatggtga gttcatggat tcaagatgaa tcagcagccc tcagaaccgc ttcgcgaacc
     2221 tgagggctgg cacccggaga ggtcagtcgg agcatgagaa gagagagggc acccggcacc
     2281 cagagaagtc agtcggagca tgagaagaga gggcacccgg cagccgagcc gattggtaag
     2341 gaggttgcga agggatgcga gtgaggaagc ggtggtcgtt cgcagtggcc ggagcaatgg
     2401 ctgcaattct cattgcgctc ctttgctatt caagtttt
//
LOCUS       DAOQUC010000558         2437 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_52833_length_2437_cov_2.869857, whole genome shotgun sequence.
ACCESSION   DAOQUC010000558 DAOQUC010000000
VERSION     DAOQUC010000558.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2437
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52833_length_2437_cov_2.869857"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..601
                     /locus_tag="PKH07_10035"
     CDS             <1..601
                     /locus_tag="PKH07_10035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35321.1"
                     /translation="MALSQAPASKPEPAQTKGKTPKSSVSTQPSEEVLELSRQVGSLK
                     EAVSQKDEQIERFKRMVAGAQQAEASASSRAEALTTKLIEWDRLLNERAQELKTQASR
                     IRENELKNREYVSSLQRQVAELRSRLERSGVMPSDATATVSVQPTQEDTRGVEIKTED
                     DSDESQGETDVEIPPMELLNEFGELEDRATVPESATKEP"
     gene            641..1417
                     /gene="larB"
                     /locus_tag="PKH07_10040"
     CDS             641..1417
                     /gene="larB"
                     /locus_tag="PKH07_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014183140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HNT35322.1"
                     /translation="MNRKALESLLKSVQSNDITIEDALQRLRNLPYEDLEFVKIDHHR
                     ALRQGYPEVVFCPGKTVEQAREIITRLWDSGSDVLATRGSEELFHLVQSIVPQAQFHH
                     LSKAIVLQHEPSHELRPGPILVVCAGTSDLPVAEEAAVTAKAMGNEVQMLTDVGVAGI
                     HRLLNHRDLLWAATVVIVVAGMEGALASVVGGLVNIPVIAVPTSIGYGASFNGLAALL
                     AMLNSCAAGVTVVNIDNGFGAGFAASQINRLRERPVKGRC"
     gene            complement(1418..2086)
                     /locus_tag="PKH07_10045"
     CDS             complement(1418..2086)
                     /locus_tag="PKH07_10045"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HNT35323.1"
                     /translation="MAKQFAVIGLGRFGQKLAVSLEERGGEVLAIDSDARRVEEIKDK
                     VSAAIRMNGTDESALRAQGVDKVDVAVVCIGESFEANLLTTVIVKRMGVPLVITRTSA
                     EVHSQILSLVGADKVIFPEEDVAVKLAQNLTIETILDYVPISDEHTAAQVSAPKKFWG
                     KTLGELDMRRKFGVNLVAIKRMEGSERPGFVDDMPGAETAIAQGDILIVVGRGEDIET
                     MASS"
     gene            complement(2105..>2437)
                     /locus_tag="PKH07_10050"
     CDS             complement(2105..>2437)
                     /locus_tag="PKH07_10050"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HNT35324.1"
                     /translation="EIWSRRIPASVVESAAIVVFLAAGLVATSVLVLSMTEDASLRDI
                     IFEEVSAFGTVGLSLGLTPKLTDAGKMVIILSMLVGRLGPITLVLSLAQPRRRVGYDL
                     PDAPIMIG"
BASE COUNT          559 a          698 c          663 g          517 t
ORIGIN      
        1 catggctctg tctcaggctc ccgcttccaa gcccgaaccg gctcagacca aaggcaagac
       61 acccaagtca tccgtcagta ctcaaccctc ggaggaagtt ctcgaactca gccgccaagt
      121 gggaagcctc aaggaggccg tttcgcagaa agacgagcag atcgagcggt tcaagcgcat
      181 ggttgcgggg gcacagcagg cagaggcttc ggcatcaagc cgcgctgagg cactgaccac
      241 gaagttgatc gaatgggaca gactgctcaa tgagagagcc caagaactca agactcaggc
      301 gtctcggatt cgtgagaacg agttgaagaa tcgggaatat gtcagcagcc ttcagaggca
      361 ggtggccgag ctgcgatcgc gtctggagcg ttcgggagtt atgccttccg atgcgacagc
      421 gaccgtttcc gttcagccca cccaagagga cacacgcggc gttgagatca aaaccgaaga
      481 tgactccgat gaatcacaag gcgagacgga tgttgaaata ccccctatgg agctgctgaa
      541 tgagttcggg gaactggaag accgcgccac cgttcctgag tctgccacca aggaaccttg
      601 atctgccaga gtccgtctgc tcacaggagt cgcatcgaag atgaaccgca aggcgcttga
      661 atcccttttg aagagcgttc agtcaaacga tattaccatt gaagatgctc ttcagcgact
      721 ccgaaacctt ccctacgaag atctggaatt cgtcaagatt gatcatcaca gggcgttgcg
      781 tcaagggtac ccggaagtgg ttttctgccc tgggaagacg gtcgaacagg ccagggaaat
      841 catcacccgc ttgtgggatt ccggctcaga tgttctggcg actcggggct ccgaggaact
      901 cttccacctc gttcagtcta ttgttccgca ggcgcagttt catcatctct cgaaagcaat
      961 cgtcctgcaa cacgaacctt cacacgagtt gcgcccaggt cccatcctcg ttgtctgcgc
     1021 agggacgtca gacctgcccg tggcagagga agccgcggtg acggccaagg ccatgggcaa
     1081 cgaagtccag atgctcaccg atgtaggcgt ggcagggata cacaggcttc tgaatcatcg
     1141 ggatctcctc tgggcggcga cggtggtcat tgtcgttgcc ggcatggaag gagccttagc
     1201 gagcgttgtt ggagggctgg tgaacattcc tgtgatcgca gttccaacca gtattggcta
     1261 cggtgcaagt ttcaatggac ttgcggcgct gctcgccatg ctgaacagtt gtgcggcagg
     1321 tgtgactgtg gtcaacattg acaatgggtt tggagcgggt tttgcagcat cacagatcaa
     1381 ccgtcttcgg gaacgtcctg tgaaaggtcg ttgctgacta tgacgaggcc atcgtctcga
     1441 tgtcctcacc tcgtccgact acgatgagaa tgtctccctg agcgatggct gtctccgcac
     1501 caggcatatc atccacaaaa ccaggccgtt cagacccctc catacgcttg atcgcgacaa
     1561 ggttgacacc gaattttcgg cgcatatcca attctccgag agtctttccc cagaacttct
     1621 tgggggcgct gacctgcgcg gcggtgtgtt catcggagat cggtacgtag tccagaatgg
     1681 tctctattgt cagattctgg gcgagtttga cggcaacatc ctcttcggga aaaatcacct
     1741 tgtctgctcc aaccaggctc agtatctggc tgtgaacctc tgccgaggtc ctcgtgatca
     1801 ccaggggaac gcccatcctc ttgacgatga cggtcgtcag caaattggct tcaaaactct
     1861 ccccaatgca gacaacggcg acatcaacct tatccacccc ctgtgcgcgc agagcactct
     1921 cgtcggtgcc attcatccga attgctgcag acaccttgtc cttgatttcc tcgactcgcc
     1981 gtgcgtcgct gtctatggca agcacttccc cgcctctctc ttcgagcgat acagccagtt
     2041 tctgcccaaa tcttccgagg ccgatgaccg caaactgctt agccattcag atgtacctcc
     2101 taagctaccc aatcattatc ggggcgtcgg gtagatcgta gccgactcgt cttctgggtt
     2161 gggcaaggga cagtaccagt gtgattggtc ccagcctccc aaccagcatg gaaaggatga
     2221 tgaccatctt gccggcatcc gtcaacttgg gagtcaaccc cagactgagc cccacggtgc
     2281 caaacgcaga gacttcctca aaaataatgt cacgaagaga ggcgtcttcc gtcatggaaa
     2341 gcacaagtac tgaggtggca acaagcccgg ccgccagaaa aaccacaatg gcggcgcttt
     2401 caaccacaga agccgggatg cgccttgacc atatttc
//
LOCUS       DAOQUC010000559         2436 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_52863_length_2436_cov_3.293154, whole genome shotgun sequence.
ACCESSION   DAOQUC010000559 DAOQUC010000000
VERSION     DAOQUC010000559.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52863_length_2436_cov_3.293154"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="PKH07_10055"
     CDS             <1..197
                     /locus_tag="PKH07_10055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35325.1"
                     /translation="QQRNHRRARKPEQVQQAVRECMNAAKVGGGYVIMPTAAPINIPL
                     AKKTEDNYRTFIETALEYGR"
     gene            211..1302
                     /locus_tag="PKH07_10060"
     CDS             211..1302
                     /locus_tag="PKH07_10060"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNT35326.1"
                     /translation="MKLNNIPLRQPKPYAERFIGILTGRAEGSKPPLVEYLVDDTLMK
                     PILRDLLERPWVDPVDRSTLESYLDNFICFWHRMGYDFVRFETSLPFPMHRLITKDTG
                     SDKQRGWVDEHQGMITTWEEFEKYPWPEVEKMDFFPYEYINSHLPEGMGLMTCHAGGI
                     YEHLSYLMSYEGLAFALFDNPELVRAVTDRLGELFTGFYRHLVELDRVVAIFPGDDMG
                     FRSGTLIAPQSLREYCLPWHQRFAEMAHQKGLRYFLHSCGCLESIMEDLIETVKIDGK
                     HSYEDAIIPVEKFQEKYGSRIAILGGVDVNTLSSGTLEEVRQRVRFLLETCGAKGRYA
                     LGSGNSIPSYVPLENYLTMVDEVVGFQGC"
     gene            1325..2212
                     /locus_tag="PKH07_10065"
     CDS             1325..2212
                     /locus_tag="PKH07_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019004066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HNT35327.1"
                     /translation="MKKTGDIQIRDPFVVPIEQEKRYYLYGTTDVNCWGPPGVGFDVF
                     TSTDLKHWKGPLPAFRPPKDFWSDHNFWAPEVHRVSDRYFMFASFKAKDICRGTQILV
                     SDSPKGPFSPHSERPLTPSHWESLDGTLYFDKDNYPWMVFCHEWIQVHDGEICAVRLS
                     PEFDQALGEPILLFRASEAPWVLKPEETDGATEKPNLVTDGPFLHRTKNGTLLMLWSS
                     FSATGYTTGVARSLSGEIHGPWLQDPEPIYAKDGGHAMLFRTFEGQLMLSLHTPNKSP
                     HERALFLPIAECEGRLHLL"
     gene            complement(2236..>2436)
                     /locus_tag="PKH07_10070"
     CDS             complement(2236..>2436)
                     /locus_tag="PKH07_10070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35328.1"
                     /translation="AFEPTAHREFLTLTTIDDVSVAFEKQSSKGVTTLTLAMPDSKAE
                     LTLEPKRFKVSTKGFRRSFADE"
BASE COUNT          572 a          650 c          641 g          573 t
ORIGIN      
        1 cacaacagcg caatcatcgt cgtgcgcgaa agccagaaca ggtgcagcag gctgtccgtg
       61 agtgtatgaa cgcggcgaaa gtcggaggtg gctatgtcat catgccgacc gctgcaccaa
      121 ttaacatccc acttgccaaa aaaaccgagg acaactatag aacgttcatc gaaactgcct
      181 tggagtatgg cagataactt ggagtcaaga atgaagttga acaacattcc tcttcgtcag
      241 ccgaaaccat atgccgaacg atttatcggg atcctcacgg gtcgcgcaga gggttcgaaa
      301 cctccgctcg ttgaatacct ggtggacgac acgctgatga aaccaatcct gcgagacttg
      361 ctcgaacgtc cgtgggttga tcctgttgat cgttcaacgc ttgagagtta tctggacaac
      421 ttcatctgtt tctggcaccg gatgggatat gactttgtcc gctttgagac aagtcttccc
      481 ttccccatgc atcgtctaat cacaaaggac accggttcgg ataagcagcg aggctgggtg
      541 gatgagcacc agggaatgat caccacatgg gaagagtttg agaagtatcc ctggcctgaa
      601 gttgaaaaga tggacttctt cccctacgag tacatcaact cacacctgcc cgaaggcatg
      661 ggtctgatga cctgccacgc gggaggcatc tatgagcacc tctcctatct catgtcctat
      721 gagggcttgg cttttgcctt gtttgacaac cctgaattgg tcagagccgt gacagacaga
      781 ctcggggaac tcttcactgg cttctatcgg cacctggtcg aactggatcg cgtcgttgcc
      841 atctttccag gggacgatat gggatttcgg tcgggaacgc tgattgcgcc tcagtcgctc
      901 cgtgagtact gcctgccctg gcatcaacgc ttcgccgaga tggctcacca gaagggactc
      961 cggtatttcc ttcattcctg tggttgtctg gagtccatca tggaagacct gatcgagacg
     1021 gtgaagatag acggcaaaca ctcttatgaa gatgccatca tccctgtaga gaagttccag
     1081 gagaagtacg gcagtcgtat cgcaatactg ggtggagttg atgtcaatac cctctcatcg
     1141 ggaacactag aagaagtgcg tcagagggtt cgctttctct tggagacctg tggtgcgaag
     1201 ggtcgctacg cgctcggttc cggcaactca attccgagtt atgttcctct cgagaactac
     1261 ctgaccatgg ttgatgaagt cgttgggttc caaggctgtt gatgagaaag agagtccttc
     1321 atctatgaag aagaccggtg acattcagat tcgcgatcct ttcgtagtgc caatcgagca
     1381 agagaaacgc tactacctct atggaacaac ggacgtgaac tgctggggac ccccgggtgt
     1441 cggctttgat gtgtttacca gcacagattt gaagcactgg aaagggcctc ttccggcatt
     1501 tcggccacca aaggatttct ggtctgacca caacttctgg gcaccggaag tccatcgcgt
     1561 gagcgatcgc tatttcatgt ttgccagctt caaggccaag gacatctgcc gcggcaccca
     1621 gattctcgtc tcagacagtc ccaaaggccc tttctctccg cacagcgagc gacccctgac
     1681 accctctcac tgggaaagcc tcgacggcac tctttacttt gacaaagaca actacccttg
     1741 gatggttttc tgccatgaat ggattcaggt tcacgatgga gagatctgcg ctgttcgcct
     1801 gtcacctgaa ttcgatcagg ctttgggtga accgattctg ctctttcgtg cgtcagaagc
     1861 gccctgggtt ttgaaaccag aagagaccga cggtgcgact gagaaaccca atcttgtcac
     1921 ggatggcccc tttcttcatc gcacaaagaa tgggacgctc ctgatgctgt ggtcaagttt
     1981 cagtgcgacg ggatacacaa caggcgtggc acgctcgctt tcgggtgaga tccacggacc
     2041 ttggctgcag gatcccgaac cgatctatgc caaagacggc ggacacgcaa tgctctttag
     2101 aaccttcgag ggtcaactca tgttgagcct tcacacaccg aacaagtctc cgcatgagcg
     2161 cgctctgttt cttccgatcg ctgagtgcga aggacggctc cacctgctct gacacagaga
     2221 tgattcctct gcagtctatt cgtctgcaaa acttcggcga aagcctttcg tagagacctt
     2281 gaaacgcttg ggctcgagcg tcagctctgc tttggagtca ggcatcgcaa gcgtaagagt
     2341 cgtaacaccc ttcgacgatt gcttctcgaa ggcaaccgac acgtcatcaa tggtagtcag
     2401 cgtcaggaat tcccgatgcg cagttggctc aaaagc
//
LOCUS       DAOQUC010000560         2436 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_52874_length_2436_cov_2.793784, whole genome shotgun sequence.
ACCESSION   DAOQUC010000560 DAOQUC010000000
VERSION     DAOQUC010000560.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52874_length_2436_cov_2.793784"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1179
                     /locus_tag="PKH07_10075"
     CDS             <1..1179
                     /locus_tag="PKH07_10075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35329.1"
                     /translation="LVYQDTHNVTFRNFILSPHPGSSYIMNALVTINDPASPAMTRNW
                     VKFYDCVFTEAFATGEPMVTDKASALANKDALPGARSTRMSSTAGKCILKGATNNGLQ
                     SVRLDNCVFYGPPQIGFELNVRTGDEIQVHDTLVVLCGFSAFRCLGNAVAHHSFTGTD
                     VGAGPTQCTAIINSPSTTATTDDNGKALRLASSAAATTTISNVLISNSFLAGNAFIGG
                     ININGNQGNVSIEDVIIDTYGTAILVQPANTCTVSNATLHTSGAGGAGIAFTAAAAGS
                     LTVADTIFSGAGTKIVGDLPTGGLSVNYSAFVENGSDAITARDAGQSITYGANIITAD
                     PQYVSKDATSANAFDVRGLTYKGAGSAGSNLAGGADFILPPDTPTHSTSVDPSWELYD
                     "
     gene            complement(1661..1933)
                     /locus_tag="PKH07_10080"
     CDS             complement(1661..1933)
                     /locus_tag="PKH07_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005671138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HNT35330.1"
                     /translation="MNKAELIEAVASDAGLTKKDAAAAVDAVFGNIVKSLKKKQTVQL
                     VGFGTFLVAQRKARSGVNPATKEKIKIKAAKVPKFKPGKALKDAVN"
     gene            complement(2089..>2436)
                     /locus_tag="PKH07_10085"
     CDS             complement(2089..>2436)
                     /locus_tag="PKH07_10085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35331.1"
                     /translation="VQSAGLFSRTQPFANYGQVPDVAYAAMRTAVGECTGLIEATVPV
                     LVENKEVARTTVGYFILQVLEHVEPDMTAYHEKERATLLNSMLNERRNAAIQDWLKQR
                     RAQSKIDIKMKDR"
BASE COUNT          530 a          691 c          611 g          604 t
ORIGIN      
        1 ttggtctacc aggacacgca caacgtgacc ttccggaatt tcatcctcag tccacaccct
       61 ggaagctcct acatcatgaa tgccttggtt accatcaatg accctgcttc tcccgcgatg
      121 acacggaatt gggtcaagtt ctatgactgt gtcttcaccg aagctttcgc taccggagag
      181 cccatggtta cggacaaggc atccgccttg gcaaacaaag atgctcttcc gggtgcacga
      241 agtactcgga tgagctctac ggccgggaaa tgtatcctca agggtgctac aaacaacggt
      301 cttcagtcgg ttcgtcttga caactgtgtt ttctatggac caccccaaat cggttttgaa
      361 ctaaatgttc gaacgggtga cgagatacag gttcatgaca cgctggttgt tctgtgcgga
      421 ttcagcgcat ttcgatgtct cgggaatgca gtggctcatc actcgttcac tggcaccgat
      481 gttggtgcag gcccgaccca gtgtactgcg atcatcaatt cgccgtccac gactgcaacg
      541 actgacgaca acggaaaggc gcttagactg gcatcgagtg ctgcagcgac taccactatc
      601 agtaacgttc tgatttcaaa cagtttctta gctggcaacg cattcatcgg tggaatcaat
      661 atcaatggca accaaggcaa tgtttccata gaagatgtga tcattgacac ctacggtaca
      721 gccatcctgg ttcagccggc aaacacatgc acggtaagta acgccacact ccatacttct
      781 ggcgctggtg gagcgggtat cgccttcact gccgctgctg ccggcagtct gactgttgcg
      841 gacactatct tcagtggcgc aggtaccaag atcgttggcg atcttcccac cggtggcttg
      901 agtgtcaact actctgcgtt tgtcgagaac ggatccgatg ccatcacggc tcgtgatgct
      961 ggacagtcga tcacttacgg tgcaaacatc atcactgccg atccgcagta cgtcagcaaa
     1021 gacgcaacat cagcgaacgc atttgacgtt cggggactca cctacaaagg tgctggatct
     1081 gctggctcga atctcgcggg tggcgctgac ttcatccttc ccccagacac accaacgcac
     1141 tcaacgagcg ttgatccgtc ctgggagttg tacgactgag cccatttcct gccatataga
     1201 atgctgtggc aggattgttg tcattcgccc gcaggtgtcg gtttttcccc catgcctgcg
     1261 ggtggattct ttttggggga tcgagacagg gctggctgct aacctttcta tcaggtctct
     1321 gtgtaagatc aaggcgcagg cttcgaccac gagcaaagat gctcgtggca caggagtggt
     1381 gccagctcag ttacactggc gatagagcca ggatatgctt cggaagcaaa gcggagtctt
     1441 cggagcgacg ctaccgaagc ccgctttctg tctggtcccg cttcggcagc gtcgcagagc
     1501 ctctgcaacc gaagcatacc tcccccccct tccgtaactg agccagtacc aggagtgacg
     1561 gatttccacg tgaccgctta gatataaaaa ggaaagggga catactcttc ggattcagac
     1621 cgtaagaaat gtcccctgtc aataacagtt tgacgcgata tcagttcacg gcgtctttca
     1681 gtgccttccc gggcttgaac ttcgggactt tggcagcctt aatcttgatc ttctccttcg
     1741 ttgccggatt gactccagaa cgagctttgc gctgtgcgac caggaaagtg ccgaagccaa
     1801 ccaactgaac agtctgcttc tttttcagac tcttcacgat gttgccgaaa acggcatcaa
     1861 cggcagcggc ggcatccttc ttcgtcagac cagcatcact ggcgaccgct tcgatcaatt
     1921 ccgctttgtt cacctatact tccctccctt cttagagacg gcatatttga ttctgcccta
     1981 taataggtct gtccacccct gttgtcaaga gggactaggg cttttcaaca aagattttct
     2041 tgctcccaat ctgcactcac ttcgtcacgg cacaaccgga ttggtggctc accgatcctt
     2101 catcttgatg tcaattttgg actgcgctct ccgctgtttc agccagtcct gaatcgctgc
     2161 atttcgtcgc tcattgagca tgctgttcaa aagagtggcg cgctctttct cgtgataggc
     2221 ggtcatgtcc ggttcgacat gctcaagcac ctgaaggatg aaatatccaa cggtcgttcg
     2281 tgccacttcc ttgttctcca cgagtacggg cactgtggct tcgatgaggc ctgtgcactc
     2341 tcctacagcc gtcctcatcg cggcgtaggc cacatcgggg acttgcccgt agttcgcgaa
     2401 cggctgagtg cggctgaaca agccagcgct ttgaac
//
LOCUS       DAOQUC010000561         2434 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_52922_length_2434_cov_3.592266, whole genome shotgun sequence.
ACCESSION   DAOQUC010000561 DAOQUC010000000
VERSION     DAOQUC010000561.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2434
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52922_length_2434_cov_3.592266"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..456)
                     /locus_tag="PKH07_10090"
     CDS             complement(<1..456)
                     /locus_tag="PKH07_10090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35332.1"
                     /translation="MKKLLLVIALTLCVSVSYGAMTLPLEDNFNAGGPDHTWDFYGGT
                     YSAAGSGTHIMTGFNGRDCLAVDDGSGYQLVFPHADEGTQSAQVMVEAWQYIPSNDSG
                     TTWARIGVGCRVKGTGWADARDSGYWVYADTDSDNYFTVGVCFRNWVAPG"
     gene            683..1246
                     /locus_tag="PKH07_10095"
     CDS             683..1246
                     /locus_tag="PKH07_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020589632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HNT35333.1"
                     /translation="MTHLRSERPRRDQTDSVSKGKGRSSMFVPTLIMAGIALVLVGIG
                     YHRGQGQHIVGMKCGLRLTVEVLPLLLFAFIVAGMIQTLVSREALARWVGGQSGLSGI
                     LIGTVAGGLCPGGPYVSLPIVASLLKVGASTGVMVAFLTSWSLWAVSRIPMEVGILGW
                     KFTLVRLASTFFFPPIAGLIAQKMTQG"
     gene            1307..>2434
                     /locus_tag="PKH07_10100"
     CDS             1307..>2434
                     /locus_tag="PKH07_10100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35334.1"
                     /translation="MDTHTLAVLEFDKIRRLIRKCLATPIGAQRVDALEPIADPSELD
                     RRRTRLREMRHLLEGSSGLPLDGARDIRKSLETARIEGSILEPGALLDILTTSTASGK
                     VRRSIVASDRRIPELREMVSVLEPDPPLQNAISESIAADETIKDSASKSLAKIRKELS
                     ALRATITERVQQILKRRSVQEILQENFYTQCEGRYVVPIQAQFKGRFRGIVHDRSDTG
                     ATVFVEPAEIVPLGNKLRELEVEERRECNRILRSLTSKVRHSYSQLSINQEILADLDF
                     LQAVSRYSETYHMVQPDLAASGSLRLHGARHPLLLAQMPFEKVAPLDMDLAPEKSGSA
                     KTVVVVTGPNTGGKTVVIKTAGLLVLMALAGLDVPAEHGTII"
BASE COUNT          552 a          678 c          642 g          562 t
ORIGIN      
        1 ccccggtgcg acccagttcc tgaagcagac acccactgtg aagtagttgt ccgagtcagt
       61 gtcggcgtac acccagtatc ctgaatcccg tgcatctgcc cagccagttc ccttcacccg
      121 acagcccaca ccgatccgag cccaggttgt ccctgagtcg ttggatggaa tgtactgcca
      181 ggcctcaacc atgacctgag ccgactgtgt tccctcatcg gcatgcggga acacgagctg
      241 atagccagag ccatcatcaa ccgccaggca atcccgaccg ttgaacccgg tcatgatgtg
      301 ggtgccagaa cccgctgcac tgtaagtgcc accataaaag tcccaagtat gatccgggcc
      361 tcccgcgttg aaattatcct ccaaaggaag agtcatggct ccgtaggaaa ctgacacgca
      421 cagagtcaat gcaattacaa gtaagagttt tttcatatct ctctcacctc cctcgctcac
      481 agaataacat tacacctcta ctaatcttgg caatctacgc ctctcagttg tgaaaagtca
      541 aacattatgt tcaacggctc ggaactgtcg agcatagtca ctggtctacg cgcctcttcg
      601 gtgtctcttt gaaccgccgg agcgtttctg atactcttca atgtgccggg gccctggacg
      661 tcccgtcagg agaacatctg gcatgacaca tctgcgttct gagcggccaa gaagagatca
      721 aacggatagc gtctcaaagg gaaaggggcg atccagcatg tttgtgccaa cactgattat
      781 ggcaggtatt gctctggtat tggttggtat cgggtatcac cgtggacagg ggcaacatat
      841 cgtcggcatg aagtgcggat tgagactgac ggtggaagtg ctcccactgc tccttttcgc
      901 cttcatcgtt gcagggatga tccaaacgct tgtctcaagg gaggcccttg ccagatgggt
      961 tggtggacag tcggggttga gcggaattct gattgggact gtggcgggtg gtctctgccc
     1021 cggaggcccg tacgtcagcc ttccaattgt cgcaagcctt ctgaaagtcg gtgccagcac
     1081 aggcgtcatg gttgctttcc tcaccagttg gtcgctctgg gcggtctcca ggattccaat
     1141 ggaagtcggc attctgggct ggaaattcac tttggttcgc ctggcaagca cattcttctt
     1201 tccccccatt gcaggactta ttgcacagaa aatgacgcag ggatgaaccc ttgagagaac
     1261 ggctttccag tagtccctca ctttgctaca ttagacccga ttgaacatgg atacacatac
     1321 tctggctgtt ttggaattcg acaagatcag gcgcttgatt cgcaagtgtc tcgccactcc
     1381 catcggggca caacgcgtgg atgctctcga accgattgcc gatcccagtg aactcgaccg
     1441 ccgccgcact cggttgcgtg agatgcgtca tcttctggaa ggatcgtccg gtctgcctct
     1501 ggacggagca cgagacattc ggaaatctct ggaaactgcc cggattgagg gctccatcct
     1561 ggagccgggt gccttgctcg acatcctcac aacaagcacg gcttcgggta aagtcaggcg
     1621 ttcgattgtg gcaagtgaca ggagaatccc agaactgcgt gagatggtct ccgttctgga
     1681 gcctgacccg cctttgcaaa acgcaatttc ggaatccatc gcggcagacg aaaccataaa
     1741 ggattccgcg tccaagtcgc tcgcgaaaat ccggaaggaa ctctcggcac tgcgtgcaac
     1801 gatcacggag cgagtacagc agatattgaa acgtcgcagt gttcaggaga ttctccagga
     1861 aaacttctac acccaatgcg agggacgcta tgtcgttccg atccaggctc aattcaaagg
     1921 ccgatttcgc ggcattgttc atgatcgctc cgataccggt gcgacggtct ttgtcgagcc
     1981 tgcggagatt gtgcctctgg gaaacaaact gcgtgaactg gaagtcgaag agcgacggga
     2041 atgcaaccga attcttcggt cgctgacttc aaaggtaagg cactcctatt ctcaactcag
     2101 catcaatcag gagattctgg cagatctgga ctttcttcag gctgtcagcc gatactcaga
     2161 aacctatcac atggttcagc ccgaccttgc agcttcaggt tcgttgcggc ttcatggagc
     2221 tcggcatccc ttgcttcttg ctcaaatgcc ctttgagaaa gttgccccgc tcgatatgga
     2281 tttggctccc gagaaatctg gttcggccaa aacagtagtg gtagtcacag gccccaacac
     2341 cggtggcaag actgtcgtca tcaagaccgc cggacttctg gttttgatgg cgctggcggg
     2401 actcgacgtc ccagccgaac atggaacaat catc
//
LOCUS       DAOQUC010000562         2432 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_53010_length_2432_cov_3.043753, whole genome shotgun sequence.
ACCESSION   DAOQUC010000562 DAOQUC010000000
VERSION     DAOQUC010000562.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53010_length_2432_cov_3.043753"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1503)
                     /locus_tag="PKH07_10105"
     CDS             complement(<1..1503)
                     /locus_tag="PKH07_10105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35335.1"
                     /translation="MKSEISATLNGATLTFDAQTGGITKLSYPGPGAMIETTPDKAGL
                     LDLAYPVSDFQPLRLASRFSKGASFDVQDDRVTVRYERLGGSRSVFEQPGAVAATVTL
                     EAATDGKSIVMSCTVENHSDVTVRQVIFPDLMGLIPFEGPDQTEFRTASFVSKPFREL
                     VPDEGRLSLHFCQDQSCGMVEYKSGGMFSEMWLRWMDLGGLKGGLSLFQKAWGWDVRP
                     TVRLHLDPNEPKLRLLCTHLLTIEPGAKWESGEFVLTPHANGWAKGIEPYAEYARSHL
                     NKEFPMPKHVREGLGFRTIWMSKGFPKDPQDTVWKFEDLPALAEDSLRHGIAEMVMWS
                     YTEGFKLPIPKPSSNLGTEQDLVNGIKRARELGVNVAPFISVLQAGHDTGAKYGLTIP
                     TEGGWPQHPEAIPPFQAPYISLYQSAGVDTNNELWQKEVLESCLHYVNIGIPSLSWDQ
                     YWTEPNMNIIRIMSKVRAEAKKADPESTFSTEELWNMEIDADYIDYTWNWG"
     gene            complement(1661..>2432)
                     /locus_tag="PKH07_10110"
     CDS             complement(1661..>2432)
                     /locus_tag="PKH07_10110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35336.1"
                     /translation="WQKEVLESCLHYVKIGIPSLSWDQYWTEPNMNIIRIMSKVRAEA
                     KRLDPESTLSGEEMVNLEVDADYLDFTWNWGGFRNCQAFTNVFPSPRISCIVTEAPYV
                     VKAAFLDNLYLNVMPRKTESVNGSDWIYKYQAMSEALKECAALRSQFLEYFTEGRLIG
                     SCLLSEPCPNTHIASYIMPDRALLLVMNQGGAGPVHFKCDLAPWLDSDKGDYTIRQYD
                     STGKLTDTLHLKNGNWNGKTPSVETFEITLFEITGGEH"
BASE COUNT          548 a          657 c          637 g          590 t
ORIGIN      
        1 cccccagttc caggtgtaat caatgtagtc ggcatcgatc tccatattcc agagttcttc
       61 cgtggagaag gtggattcgg gatcagcctt cttggcctca gctctcacct tcgacatgat
      121 cctgatgata ttcatgttcg gctcagtcca gtactgatcc catgaaagcg aggggatgcc
      181 gatgttcacg tagtgcaggc aggattccaa gacttccttc tgccacagtt cgttgttggt
      241 gtcgacgccg gcactctggt agagcgagat gtacggggcc tggaagggcg gaatcgcctc
      301 gggatgctgc ggccatccgc cttcggtcgg aatggtgagg ccatatttgg caccggtatc
      361 atgacccgcc tgcaaaacgc tgataaaggg cgcaacattc actcccaact ctctggctct
      421 cttgattcca ttgacgagat cctgttccgt ccccaagttt gaggaaggtt tgggtatggg
      481 aagtttgaag ccttctgtgt aactccacat aaccatttcc gctatgccgt gacggaggct
      541 gtcctctgcc agtgcgggca ggtcttcgaa cttccagaca gtatcctgag gatcttttgg
      601 aaagccctta ctcatccaga tcgtgcgaaa ccctagacct tcgcgcacat gctttggcat
      661 ggggaattcc ttgttgaggt ggcttctcgc gtactcggcg taaggttcaa ttcccttggc
      721 ccaaccgttt gcgtgaggag tcagaacgaa ttctccgctc tcccatttcg cgcccggttc
      781 gatggtcaaa aggtgagtgc aaagcagccg cagttttggc tcgtttggat ccagatgcag
      841 acgcacagtg gggcgaacgt cccatcccca ggccttctga aaaagactca gaccaccctt
      901 aagtccgccc agatccatcc aacgcaacca catttcgctg aacatcccgc ccgatttgta
      961 ctcgaccatg ccgcaggact gatcctgaca gaaatgaagg ctcaaccgtc cctcatcagg
     1021 taccagttct ctgaagggtt tgcttacgaa ggaggcggtt cggaattcag tttgatccgg
     1081 cccttcgaaa gggatgagtc ccatcagatc agggaagatg acctggcgaa cagtcacgtc
     1141 ggagtgattt tcaaccgtgc aactcatgac gatggacttc ccatcagtgg cggcttcaag
     1201 cgttaccgtc gcggctacgg ctcccggctg ttcgaagacc gaacggctgc caccgaggcg
     1261 ctcgtagcga acggtcaccc gatcatcttg aacatcgaac gatgcacctt tcgaaaaacg
     1321 cgaagcgagc cgcagaggct ggaaatcact gacgggatag gcgagatcca gaagtcccgc
     1381 cttatctgga gtagtctcaa tcatggctcc agggccggga tagctgagtt tggtgatgcc
     1441 accggtttgt gcatcaaagg tcagtgttgc accattgagt gtggcgctga tctcagattt
     1501 catcggttca tctccttggg ctgacgagat aattgcagct cctgtcacaa taataaccca
     1561 cagtcttttc atccacatag catatttccc ttcacggcag agatagtgcg ggttgtgccc
     1621 gacaaaaaca agcagtcggg ctaagcccga atgacggttt ctaatgctct ccgccggtga
     1681 tctcaaacag ggtgatttca aatgtctcca ctgatggagt cttgccgttc caattgccgt
     1741 tcttcaggtg aagcgtatcc gtcaatttcc ctgtcgaatc gtattgcctt atcgtgtaat
     1801 cgcctttgtc ggaatcaagc cagggtgcca aatcgcactt gaagtgaact ggccccgccc
     1861 cgccctgatt catgaccaac aacaatgcgc gatcgggcat aatgtaactg gcgatatgtg
     1921 tgttcgggca gggttcactg agcaggcaac tgccgatcaa acggccttcg gtgaagtatt
     1981 caagaaactg actgcgtaag gcggcgcatt ccttgagtgc ctcgctcatc gcctgatatt
     2041 tgtatatcca gtcagaaccg tttacagatt cggtcttgcg aggcatgaca ttcagataga
     2101 ggttgtccag gaatgccgct ttgacgacat aaggtgcttc cgtgacgatg caactgattc
     2161 gaggggaagg gaagacattc gtgaacgcct ggcagttgcg gaatcccccc cagttccagg
     2221 tgaaatcgag atagtcggcg tctacctcaa ggttcaccat ctcctcaccg gaaagggtgg
     2281 attcggggtc aagccgtttc gcctcagcgc gaaccttcga catgatcctg atgatattca
     2341 tgttcggctc agtccagtat tgatcccatg aaagagatgg gatgccaatc tttacataat
     2401 gcaggcagga ttccagaact tccttctgcc ac
//
LOCUS       DAOQUC010000563         2428 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_53119_length_2428_cov_3.723978, whole genome shotgun sequence.
ACCESSION   DAOQUC010000563 DAOQUC010000000
VERSION     DAOQUC010000563.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2428)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2428)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2428
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53119_length_2428_cov_3.723978"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="PKH07_10115"
     CDS             complement(<1..202)
                     /locus_tag="PKH07_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018267936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35337.1"
                     /translation="MKIVVCIKQVPDTTEIRINPETNTLMREGVPSVVNPFDLYALEE
                     GLMLRDQLGGTVTTLSMGPPQAA"
     gene            complement(199..1356)
                     /locus_tag="PKH07_10120"
     CDS             complement(199..1356)
                     /locus_tag="PKH07_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNT35338.1"
                     /translation="MDYFLTDDQKMIKDLARKIAEEKIKPVRAEFDESEQFPWEIMKT
                     LAQSDLFAVFVPEEYGGLGGGCFELCLVTEELSRICGGIAVCYAATALGAYPILLFGS
                     DEQKKKYLPAIAAGERLAAFALTEAQAGSDAGNIQTTATRDGDNYILNGTKQWITNGG
                     EAEIYTVVALTDRKKGARGASTFIVEKGTPGMTFGKKEKKMGIRASATREVIFQDCVV
                     SKENLIGREGMGFMVAMKTFDSSRPGIGAQSVGIAQGALDEALSFARQRVQFGQSVSS
                     IQAVQHMLSDMATQIEAARALVLATARHVDSGNPRVSKESAMAKLFASDVAMKVAVDA
                     VQICGGSGYMRDYPVEKYMRDAKITQIYEGTNQIQRNVIAQSLIRETVSKQ"
     gene            complement(1434..2228)
                     /gene="rnc"
                     /locus_tag="PKH07_10125"
     CDS             complement(1434..2228)
                     /gene="rnc"
                     /locus_tag="PKH07_10125"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007934574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HNT35339.1"
                     /translation="MKEPHLLGEERTRAISALAKTLALEIQDVTLLNRAFIHTSYANE
                     THLAEHLNNERLEFLGDAVLGFLVSEYLYRHSKTLREGDLSKMKARIVCQKVLARRAR
                     EIGLGNYLLLGRGEELSGGRYRDSILADCFEAVVGAVLLECGIDTVREFVHQNLSQEM
                     ERVRRGKGHVTDYKSILQEVVQKELNLLPKYRVVRTVGPDHDPTFEVTVSAKETILGM
                     GQGKCKKEAEQLAAQNALENIPAALNEIRSNMNLEQPTQSVPPGGK"
BASE COUNT          499 a          726 c          593 g          610 t
ORIGIN      
        1 cagcagcctg gggaggtccc atcgagaggg tcgtcactgt tcctcccaat tggtcgcgaa
       61 gcatcaggcc ctcttcgaga gcgtaaagat cgaacggatt cacaacactc ggaacacctt
      121 cccgcataag cgtgttggtc tccggattga tgcggatttc cgttgtatcg ggtacttgtt
      181 taatacagac aacgattttc actgtttgct caccgtctcc ctgatcagac tttgcgcgat
      241 gacgttgcgc tgaatctggt tggttccttc atagatttgt gtgatcttgg cgtcgcgcat
      301 gtatttctcg accggatagt cgcgcatata gccgctgcca ccgcagatct gcaccgcgtc
      361 cacagcgacc ttcatcgcga catccgaagc gaagagtttg gccatcgccg attccttgct
      421 cacccgcggg ttgccgctgt ctacgtggcg agcggtagcc aggactagag cacgcgctgc
      481 ttctatctga gtcgccatat ccgagagcat gtgctgcacg gcctgaatgc tggaaacgct
      541 ttgaccgaac tgtaccctct ggcgcgcaaa agacaacgct tcgtccagag caccctgggc
      601 aattccaaca gactgggcac caattccagg acgcgagcta tcgaatgtct tcatcgcaac
      661 catgaagccc atgccttcgc gtccgataag gttttccttg gaaacgacac agtcctgaaa
      721 gatgacttcc cgggttgccg aggcacgaat tcccatcttc ttctccttct tgccgaaggt
      781 catgcctggg gttcccttct cgacgataaa ggtggaagca ccgcgtgcgc ccttcttgcg
      841 atcggtcagg gcgacaaccg tatagatctc cgcttcacca ccattggtga tccattgctt
      901 ggtgccattc aggatgtagt tgtcaccgtc ccgtgttgcg gttgtctgga tgttgcctgc
      961 gtcactgccc gcttgagctt cggtcaatgc aaacgcggcg aggcgttccc cggcggcgat
     1021 tgccggcaga tacttcttct tttgttcatc gctgccaaac agaaggatgg gataggcacc
     1081 cagcgccgtt gcagcgtaac agaccgcgat accgccgcaa atgcgtgaga gttcctcagt
     1141 gaccagacac agttcaaagc acccaccacc gagtccacca tattcctctg ggacaaacac
     1201 tgcaaacaga tcgctctgcg cgagggtctt cattatctcc cacgggaact gctctgactc
     1261 atcaaactcg gctcgaaccg gcttgatctt ttcctcggcg attttccgcg ccaagtcctt
     1321 gatcattttc tgatcgtctg tgagaaagta atccattttc ttttcctttc ctccttctgc
     1381 cttgtggcag agtcaaaccc tctccagagg gtactttctg tttcgctgca ttcctatttc
     1441 ccgcctggcg gcacactctg cgtcggctgc tcaaggttca tgttgcttcg aatctcgttc
     1501 agcgcagcgg gtatgttctc aagagcgttt tgagccgcca actgctccgc ctctttcttg
     1561 cattttccct gtcccatacc caaaatggtc tctttcgcgc tgacggtcac ctcgaaggtc
     1621 ggatcatggt cgggaccgac ggtccggacg acacgatact tcggcaggag gttgagttcc
     1681 ttttgaacca cttcctgcag gatagacttg tagtccgtca catgaccttt gccgcgtcgg
     1741 acgcgctcca tctcctgact gagattctga tggacaaact ctcgtaccgt gtcaataccg
     1801 cattccaaca acacagcgcc tacgaccgct tcaaaacaat ccgcaaggat ggaatcgcgg
     1861 tagcgcccgc ccgacaattc ctctccgcgc cccaagagca ggtagttgcc cagaccgatc
     1921 tccctggcgc gccgtgccag aaccttctgg cagactatcc tggccttcat ctttgacagg
     1981 tcgccttcgc gaagggtctt cgaatgtctg tagagatatt ctgagaccaa aaaacccaga
     2041 actgcgtctc caaggaactc caagcgctca ttgttcagat gctccgccag atgcgtttca
     2101 ttggcgtagg aagtgtggat gaacgctctg ttgagcagag tgacatcctg tatctccagg
     2161 gcaagagtct tcgcgagcgc cgagatggct ctcgttcgtt cttcgcccaa caaatgcggt
     2221 tccttcatca caaaactctt gttattccga ctcaaaacaa gccggagtac tgttcctctt
     2281 tgtccttgtt acggtttcgt ccgcaaactt cttttgaact gtgtgtcatc aggaaccatc
     2341 aggcgtacga atcagcatcg cccacatgtc tgaaaacgcc gaggcactcc tatttttcaa
     2401 agcggctgat cgccagaacc gcgttatg
//
LOCUS       DAOQUC010000564         2428 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_53125_length_2428_cov_3.380531, whole genome shotgun sequence.
ACCESSION   DAOQUC010000564 DAOQUC010000000
VERSION     DAOQUC010000564.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2428)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2428)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2428
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53125_length_2428_cov_3.380531"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..615)
                     /locus_tag="PKH07_10130"
     CDS             complement(<1..615)
                     /locus_tag="PKH07_10130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35340.1"
                     /translation="MSYIPATVSGIETHTVGGLMETHDRTFLCLLLIALASLCVEPLA
                     VAQTTLLPPQGLHLTDTPNDNGSGVCLIWDVSPNATKAHEYVIFSSTSAEGPFHEIKR
                     IPATLSLKSDYPEFFGFSKTHKNFHCASLLYPLKSDTREPIPTWYRLAITDGTDLVFA
                     SDTVTGIPTEEWTAWNKTNNFILMLVLFAAIGYYIQRARTNPNLF"
     gene            complement(657..>2428)
                     /locus_tag="PKH07_10135"
     CDS             complement(657..>2428)
                     /locus_tag="PKH07_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008454377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNT35341.1"
                     /translation="TSTETPVPTLSETQTPTKTSTPTDSQTPTETAVPADTQTPSSTP
                     STSDTPTDTPTETPTETPTKTPTETPTETATAAPTETETPSATDTPTETAGDTPTNTP
                     TSTSTPTETPSDLPTETPTPSESPTDTETATPSATPSLTSTPTETQTPFPTDTPSGSS
                     TQTDTPTETHSPTQTPTPTMTSTETASPTITPTSLLTETPTPTATETATGTPSWTPSR
                     TATVTPTDTPTDTPSRTPTATPSNTPIDTSTPSDTPTSAPTETPSETATEAPTFTPTQ
                     TPTWTVTDTPTWTRTDTPTRTPTQTLTDTPTQTPTVTPSETPTRTPTGASTPSFVTLL
                     EVSSEGTFKMGNNGSEPFSYAYELPQHDVLLSSYYISVTEVPISGYVQFLNAGGNDDH
                     WRSQMTSTTFCGILQSGSPGSYTYSVATDRGNYPVTYVTWNDATHFCDWMTMTYTGTW
                     RLPTEAEWEKAARWVAATSTARVYPWGNSWDCNALNNGYAACVRIFRPNVVGSYPTGI
                     SPYGLLDMAGNVAEWVSDYYSATYYQSSPSENPTGPTTGTNRVVRGGSFLNGLDETRC
                     AARQNFSPTSVLNSVGFRVVRVP"
BASE COUNT          454 a          529 c          807 g          638 t
ORIGIN      
        1 gaacagattc gggttggttc tggctctctg aatgtagtag ccgatggcgg caaagagcac
       61 cagcatcaag atgaagttat tggtcttgtt ccaggcagtc cattcttccg ttgggatgcc
      121 tgtgaccgtg tctgacgcga aaacgagatc cgttccatcg gtgatggcca ggcgatacca
      181 tgtgggaatc ggttcacgcg tatcagactt gagagggtac agcagagagg cacagtgaaa
      241 gttcttgtgc gtctttgaaa acccaaagaa ctcgggatag tccgacttga gcgacaacgt
      301 cgcaggaatg cgtttgatct catgaaaagg accttctgct gaggtggatg agaagatcac
      361 atattcatga gccttggttg cgtttggaga cacatcccag atgagacaca cgccgcttcc
      421 gttgtcattg ggtgtgtccg taagatgaag accttgtggt ggcagcaggg ttgtctgcgc
      481 gacagcgagt ggctcgacac acaagctcgc aagcgcgatg agaagcaaac acaaaaacgt
      541 tctatcgtga gtttccatca agccgccgac agtatgcgtt tcaattccag atacagttgc
      601 gggaatatag ctcatccgtc ctgatatttc aaggagtttg tcccaacttg ctgcaatcag
      661 ggaacacgca cgaccctgaa tcctaccgag ttaagaacgc tggtcgggga gaagttctgt
      721 cgtgccgcgc agcgtgtctc atccaggccg ttcaggaagg accctccgcg aacgactcgg
      781 ttggttcccg ttgtcggtcc cgtggggttt tccgaaggac tggactgata gtaagtcgcg
      841 ctgtagtagt ccgaaaccca ttcggcaacg tttccagcca tgtcaagcag tccgtaagga
      901 ctgatgccgg ttggataact tccaacgaca tttggcctga agatcctgac acaggcggcg
      961 tagccattgt tcagagcgtt gcagtcccag gaatttcccc aaggatagac gcgggcggtg
     1021 cttgtcgcgg caacccatcg agcggccttc tcccactcgg cttcggtcgg aaggcgccag
     1081 gtgccggtat aggtcatggt catccagtcg caaaaatgcg ttgcatcgtt ccaggtgaca
     1141 taggtcacag gatagttccc ccgatccgtg gcaacagaat aagtgtaact gccgggtgaa
     1201 ccgctctgga ggatgccgca gaaggtggtg ctggtcatct gactgcgcca gtgatcgtca
     1261 ttgcctcctg cattcagaaa ctggacataa ccgcttattg gaacttcggt tacggagatg
     1321 tagtacgaac tcagaaggac atcatgctga ggcagttcat aggcatacga gaagggttca
     1381 gagccgttgt ttcccatctt gaacgtgcct tcggatgaga cttccaacag ggtgacaaag
     1441 gaaggagtgc ttgcgcctgt gggcgttctt gttggtgttt cagacggcgt cacggtcggt
     1501 gtctgggtgg gagtatccgt cagagtctga gtcggcgttc gagtcggggt gtctgttcga
     1561 gtccaggtgg gtgtgtcggt aacagtccag gttggggtct gtgttggagt aaacgtcgga
     1621 gcctctgtgg ctgtttcaga gggagtctcc gtcggagcag aagtgggtgt atctgatggt
     1681 gtggatgtat ctattggggt gttggagggt gtcgcggtcg gcgttcttga cggggtgtct
     1741 gtcggagtat cggtcggggt caccgtggct gttcttgagg gtgtccaact gggggttccg
     1801 gttgccgttt cagtcgccgt aggcgtagga gtctctgtca acaggctcgt cggtgtgatt
     1861 gtgggagatg ccgtttcggt gctggtcatg gtcggagtcg gggtttgcgt tggcgaatga
     1921 gtctctgttg gagtatcggt ctgagtggaa gagccagatg gggtgtccgt cgggaaaggt
     1981 gtctgggtct ccgttggcgt actggtcaag gagggagttg ctgaaggtgt tgctgtttct
     2041 gtgtccgtcg gactttcgga cggtgtcggc gtctccgttg ggagatccga aggtgtctct
     2101 gttggtgttg aagttgatgt gggcgtattt gttggcgtgt cccctgccgt ttcagtcggg
     2161 gtgtcagtgg ctgagggtgt ttcggtctcc gtcggggctg cagttgccgt ctctgtgggt
     2221 gtttccgttg gagtttttgt cggagtctct gtaggtgtct ccgttggtgt gtctgttgga
     2281 gtatcagacg ttgagggtgt cgaggaaggc gtttgtgtgt cagccggtac agcggtttct
     2341 gttggagttt gtgagtcagt cggtgtggat gtcttggttg gtgtctgcgt ctctgagagg
     2401 gttggcacag gcgtttctgt tgaggtgt
//
LOCUS       DAOQUC010000565         2428 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_53141_length_2428_cov_2.591656, whole genome shotgun sequence.
ACCESSION   DAOQUC010000565 DAOQUC010000000
VERSION     DAOQUC010000565.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2428)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2428)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2428
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53141_length_2428_cov_2.591656"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1990)
                     /locus_tag="PKH07_10140"
     CDS             complement(<1..1990)
                     /locus_tag="PKH07_10140"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HNT35342.1"
                     /translation="MEIRLDGHDWQLLPLLPFEQDWRKVWEEKWEPATGFPATSDWIS
                     GTVPGDVISDAVEAGLAPDPWVDRNSLCCEWLSERNWVYRKKFAVGTELRGKTVRLRF
                     DGVDHSCTVYLNGETLGSHEGMCLPFEFNVTDRIRFDAENTLVVVTEHAPSVSQVQGQ
                     IGRTSQARVWKARFAYDWDWCTRLIPLGIWQSVRLLVTDPVWLDDVWVRPQISDGTAN
                     IVTRATMCSSDSSVPVQKVRIRALDPDGVCFADQKTEVAIEKGRGVAEFSIAVPAPRL
                     WYPNDMGEQPLYRIVVELVDEAGNVSDTKTVSCGLRQVRMIPNENAAPDALPYTLEIN
                     GKRLFVKGWNWVPIDQMYGRVQPERYERWLNLVVHAHGNLLRVWGGGYLEREEFYELC
                     DRKGILVWQEFMQSSSGIDNAPPTDDEYLGYIENQARSLVVQRRNHPSLVIWCGGNEL
                     MEADWTPLNDAAPSLARLKSVVRELDPDRIWLPTSASGPTEGANEALAGTGKLHDVHG
                     PWQYQGPEEHYRYFEKIDPLFHSEFGTEGSTNLWTLRRFVSEKFLFPPDPSNPVWVHH
                     GAWWLHRPKLEQMFGSLETIEEFVKASQWMQAEGLRYIIESHRRRKWHCSGTCPWQFN
                     ESFPNTACTNSVDYLGWPKPVYWWVRRAYEPIHISLKYE"
BASE COUNT          582 a          723 c          583 g          540 t
ORIGIN      
        1 tctcatactt cagactgatg tgaatcggtt cgtaggcacg cctgacccac caataaaccg
       61 gcttcggcca gccgagatag tccacggagt tggtacaggc tgtgttggga aatgactcat
      121 tgaactgcca gggacacgtg ccactgcaat gccattttcg tcggcggtgc gattcgatga
      181 tgtaccgaag tccctccgcc tgcatccatt gactggcttt gacaaattct tcgatggtct
      241 ccagcgaacc gaacatctgc tccaacttgg gacggtgcag ccaccaggct ccatgatgca
      301 cccagaccgg attgctcggg tcgggtggaa agagaaactt ctcagaaaca aagcggcgca
      361 atgtccaaag atttgtcgag ccttccgttc caaactcaga gtgaaacaga ggatcaatct
      421 tctcaaagta acggtaatgc tcttcagggc cttgatattg ccaggggcca tgcacatcgt
      481 gaagtttgcc ggtgcctgcc agcgcctcgt tggcaccctc cgttggaccg gacgctgacg
      541 ttggaagcca gattcggtcg ggatccagtt cccgaaccac ggacttcagg cgtgccagag
      601 aaggtgccgc gtcgttcaga ggggtccagt ccgcttccat cagttcattg ccaccacacc
      661 agatcaccag agaaggatga ttccttctct gcacaacaag gcttcgcgcc tgattctcaa
      721 tgtagcccag atactcgtca tctgtgggcg gtgcgttgtc aatgcccgac gaagactgca
      781 tgaactcctg ccacacgaga attcccttcc gatcgcagag ttcatagaat tcctctcgtt
      841 ccagataccc tccaccccag acgcgaagaa gattgccgtg agcatggaca accaaattca
      901 gccaacgctc atagcgctca ggttgcacac gaccgtacat ctggtcaatg ggcacccagt
      961 tccagccttt gacaaaaagc cgcttgccat tgatctccag tgtatagggc aaggcgtccg
     1021 gtgcagcgtt ctcgttggga atcatccgaa cctgacggag tccgcaagag acagtcttgg
     1081 tgtcactcac attcccagcc tcatccacaa gttcaacaac aatacggtaa agtggttgct
     1141 cccccatgtc gttcggatac caaagacgag gcgctggcac agcgatcgaa aactcggcaa
     1201 ctccccttcc cttctcaatc gcaacctcag tcttctgatc ggcaaagcag acaccatcgg
     1261 gatcgagagc acggatccgc accttctgca caggcacgct tgagtcgctg ctgcacattg
     1321 tcgcgcgagt cacaatgttc gcagtaccat cggagatctg tggtctgacc cagacatcgt
     1381 ccagccaaac gggatcagtc acgagcaaac gaacgctttg ccaaatcccc aaaggaatga
     1441 ggcgtgtaca ccagtcccag tcataggcaa atcgggcttt ccacacacgc gcctgactgg
     1501 ttcgaccgat ctgtccttgc acttgagaga ccgatggggc atgctcagtg acgacaacga
     1561 gagtattctc agcgtcaaat cgaattctgt cggtgacatt gaactcgaat ggaagacaca
     1621 tcccctcatg tgatccgagt gtttcaccat tcaggtagac agtacatgaa tgatccactc
     1681 catcaaatcg cagtctgacg gtctttcccc tcagttctgt cccaaccgcg aacttcttcc
     1741 gatagaccca gttgcgctcg gaaagccatt cgcaacaaag gctgtttcga tccacccaag
     1801 ggtctggtgc cagacctgcc tcgaccgcat cggagatcac atctcctgga accgttcctg
     1861 aaatccagtc actggtggcg ggaaaaccag ttgccggttc ccacttttct tcccagacct
     1921 ttcgccagtc ctgttcaaaa ggcagcagtg gcaacaactg ccaatcatga ccatccagac
     1981 gaatctccac gcaagtctcc tttcggtttc ccagcgacac tcctggcgct gaaaaactga
     2041 ccgcccgttt ctgatactca cggaagcaaa ccttcacaca gaggaattgt caagcggaat
     2101 tggtgaagag gcgggtggga tacctcgcac ggcagcactt cttgatcaca ctctgccatg
     2161 tctcccaggg acttcgaaga agcctatctc ttccaattag cgagcgcgtg caggagcgga
     2221 catagcccac ttggctgtag actctggcta ctcatactcc cctctgctga ccatctcgtc
     2281 acgtcagagt tcacattccg acgcgctgaa acggtctcac gtgtggccta catgaattct
     2341 gtgttccaaa gttgtcaaag tggacgtata ctctcccaaa atgaaccttg gagcgttttc
     2401 caatgaaccg atgtcttttt gccctgat
//
LOCUS       DAOQUC010000566         2426 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_53210_length_2426_cov_2.792071, whole genome shotgun sequence.
ACCESSION   DAOQUC010000566 DAOQUC010000000
VERSION     DAOQUC010000566.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2426
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53210_length_2426_cov_2.792071"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="PKH07_10145"
     CDS             complement(<1..206)
                     /locus_tag="PKH07_10145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35343.1"
                     /translation="MLTCDVLRCLLVLLIPLALFLWPIGQGNEHPLLTSLSPKQWLWF
                     LSSVVFLVGALTRMYVPARMSIMP"
     gene            283..636
                     /locus_tag="PKH07_10150"
     CDS             283..636
                     /locus_tag="PKH07_10150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35344.1"
                     /translation="MNNQATALVEPGSSAEIRATHAIWFNRSPNDDMDCPSQRQRKLR
                     SRNKSFIRFSLSAYCDDFPLPVKDSDAPDAAEHMMFTVRCDHGARHLLSQRGILPFTV
                     DKIGCQGTIRMMVEI"
     gene            677..1603
                     /locus_tag="PKH07_10155"
     CDS             677..1603
                     /locus_tag="PKH07_10155"
                     /inference="COORDINATES: protein motif:HMM:NF016668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HNT35345.1"
                     /translation="MFRVFIGILVAAIFVFTRSGVSPAQTNESENSSSQSIFGDGKTR
                     LIIRVDDIGFTHAANLAMKKLAEEGCVTAASVMVTTPWLDEAVELLKQHPEISVGVHT
                     CLNCEWMPYRWGPVSPVTEVPTLVDEWGKFFGSRQQLRDNNPNVDEVEKEIRAQVDLA
                     LKKGLKISYMDHHMSAAVDTPAMRERFEKVAREYGLAISRWFGEKEGPAIYSVEPDKK
                     ADFLLAEIEKLESGNLYLMVGHPGMNTPELAVLKDIHPWAPQNMSEHREAETNALCDP
                     RFKELLAKKGIELVGYDIIKEKYLDRMARPAE"
     gene            complement(1630..>2426)
                     /locus_tag="PKH07_10160"
     CDS             complement(1630..>2426)
                     /locus_tag="PKH07_10160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35346.1"
                     /translation="HIYQRNEVKGIKYLVQGTSGDFLGAQDYSQSFVEFSTSDYYTYT
                     KIEIDGPVMTGTTKTAAGEVIDVFQIHDYILVDDGDPGFSLNGSWTYQSSPTYTEAIR
                     QDYYYVATATTETRSAIWRPSLDAGVYDVYVRYRMGSNRAPDAPYTVYYHGGSQTVLV
                     NQTILGGQWVLLGRYPFISGDSGYVKLASGPAGTGKNAIADAVKWVHVQSLSTETPTP
                     LPTWTPTATLTPTVVVTPSPTQTSLPSPTASVTPSNSGVNNWMLFR"
BASE COUNT          605 a          612 c          667 g          542 t
ORIGIN      
        1 aacggcatga tggacatccg ggcggggaca tacattcgcg tgagcgctcc cacaagaaac
       61 acaactgaac tcagaaacca tagccattgc tttggggaca gggaagtcaa aagtggatgc
      121 tcgtttcctt gcccaattgg ccacaagaac aatgccaatg gaatcagaag caccagaaga
      181 catcggagga catcgcatgt aagcatgcaa cgctttgagt cccatcgatc cacgtagaca
      241 cccaaaatag gaccaagaac aaggacagga agttgggatg cagtgaacaa ccaagcgacc
      301 gccttggtcg agcccggcag cagcgcagaa ataagggcaa cacacgccat ctggttcaac
      361 cgatcgccaa acgacgatat ggactgcccc agccagagac agagaaagtt acgttctcga
      421 aacaagtctt tcattcgatt ctcactttcg gcgtattgtg acgatttccc attgcctgtc
      481 aaggattcgg atgctccaga tgccgcggaa cacatgatgt tcacggtcag gtgtgaccat
      541 ggtgcaaggc atctgttgag tcaaagaggg attctgccct ttacagtgga caagattggc
      601 tgccaaggta caatacgaat gatggtcgag atatgatgct cggcttgtca aacgatcgat
      661 ggaggtaatc atcatcatgt ttcgtgtctt cattggaatt cttgttgcag cgatctttgt
      721 ctttacgcga tccggagtct cccccgccca aacgaatgag tctgagaact cgtcctctca
      781 atcgattttt ggagatggca aaacgcggct gatcatccga gtggacgata tcggtttcac
      841 tcatgcagca aacctcgcta tgaaaaaact tgccgaggaa ggttgcgtca cagcggcttc
      901 cgtgatggtc acaacgccct ggcttgacga ggcagtcgag cttctcaaac agcaccctga
      961 gatcagtgtt ggagtccaca cgtgtctgaa ctgcgagtgg atgccgtacc gctggggacc
     1021 tgtttctcct gtcaccgaag tgcccactct ggtggatgag tggggcaagt tcttcggtag
     1081 ccgtcagcaa ctgcgcgaca acaatcccaa cgtggacgaa gtagaaaagg agattcgtgc
     1141 gcaggtggat ctggctctga agaagggctt gaagatttcc tatatggatc atcacatgag
     1201 tgccgcagtg gatacccctg ccatgcgcga gcgttttgag aaagtggcgc gggagtacgg
     1261 tctggcaata tctcgttggt ttggtgagaa ggaaggtcct gccatctact cagtcgaacc
     1321 ggacaagaag gccgattttc tccttgcaga gatcgaaaag ttggaatcag gaaacctcta
     1381 tctcatggtg ggacatccgg gcatgaacac tcccgaactc gcggtgctga aagacattca
     1441 tccctgggca ccccagaata tgtccgagca ccgagaggct gagaccaacg cgctctgcga
     1501 tcctcggttc aaggagctgc tcgcaaagaa agggatcgag ttggttggct acgacataat
     1561 caaagagaag tatctcgatc ggatggcacg ccccgcagag taattctttg ctggacgccg
     1621 caactgcgtt cagcgaaaca gcatccagtt gttcacgccg ctgtttgacg gcgtcacaga
     1681 ggccgttgga gagggtaggc tcgtctgtgt cggggacgga gttaccacaa ccgttggtgt
     1741 caatgtcgca gtgggagtcc aggtcggcaa tggtgttggc gtctctgttg acaatgactg
     1801 aacgtgaacc cacttgacgg catccgcgat agcgttcttt ccagttcccg ctggaccgct
     1861 tgccagtttg acatagcccg aatcgcccga aatgaaggga taccgaccta gcaacaccca
     1921 ttggccgccc aaaatggtct ggttcaccaa aaccgtttgt gagccgccgt ggtagtagac
     1981 ggtgtaagga gcgtctggag cccgatttga acccatccga tagcggacat aaacatcata
     2041 gacgcccgcg tcaagacttg gtcgccagat ggcagagcgg gtttctgtgg tggccgtggc
     2101 aacgtaatag taatcctgcc gaatcgcttc cgtgtatgta gggctgctct gatatgtcca
     2161 cgaaccgttc agtgagaagc ctggatcgcc gtcatccaca agaatgtaat cgtggatctg
     2221 gaaaacatcg atcacttcac ctgcggcagt ctttgtggtg ccggtcataa caggcccatc
     2281 gatctctatc tttgtgtaag tgtagtagtc gctggtcgag aattcgacaa acgactggct
     2341 gtagtcctga gcgccaagaa agtcgccgct ggttccctgc acaagatact tgatgccttt
     2401 gacttcattt cgctgataga tgtgat
//
LOCUS       DAOQUC010000567         2425 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_53248_length_2425_cov_2.880591, whole genome shotgun sequence.
ACCESSION   DAOQUC010000567 DAOQUC010000000
VERSION     DAOQUC010000567.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2425
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53248_length_2425_cov_2.880591"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(226..684)
                     /locus_tag="PKH07_10165"
     CDS             complement(226..684)
                     /locus_tag="PKH07_10165"
                     /inference="COORDINATES: protein motif:HMM:NF021473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2190 family protein"
                     /protein_id="HNT35347.1"
                     /translation="MANNFRHRYFDPEPAMIPVESATVIEIGDFVVLSGDYAAPAGSL
                     ADAGDAAANREAAADAFVGIAMSASAVGETEDIRVGTSGVWELNQKSAAAIHFGDPVG
                     IYASADACEDQTCVEDATSPIGVCWKAKLSTSDPSVLVKILPSKINTLNA"
     gene            complement(689..1591)
                     /locus_tag="PKH07_10170"
     CDS             complement(689..1591)
                     /locus_tag="PKH07_10170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35348.1"
                     /translation="MENQNLEAELLLVECNDSPWCETSLDRQNLLLRNVCLLGPNSRN
                     GRRYPDSVIQEALGFFDGCRAYLNHPTQGQSGQGVCRDVRDLVGRYESPRIEGGKLRA
                     DLRLLPQHADLVFSLAETMPESVGMSINARGRTCVRDGEEIVQAITEIKSVDLVTDPA
                     ATRGLFESLTKRETSDKESVLWSDLDVASLTARRPDLIEQLERPLRETLHELRDQSAQ
                     QARRANVQEALRCANLPLELVSDIFIETLLEAHDDEHIHRLIEDRKSTAQQSKAVRTQ
                     SQEKGFRAGGLTDQEFYGSTRKRR"
     gene            complement(1923..>2425)
                     /locus_tag="PKH07_10175"
     CDS             complement(1923..>2425)
                     /locus_tag="PKH07_10175"
                     /inference="COORDINATES: protein motif:HMM:NF027691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha amylase family protein"
                     /protein_id="HNT35349.1"
                     /translation="ARPGVFVGDYVGSWYPDYASLGANWASRKFHAEYTWMMPDYNRT
                     GYAELVDMLAVGLYYPNVTIEEAQSVPLKDYYSVEGAADMAQKVVSNAAPLIGTLYLL
                     QYKDNPHQFVRAVQMARQKTNGVGIFDAVYLANYDWWDELETALKGPAEVLPLMPADI
                     PVEATP"
BASE COUNT          545 a          708 c          612 g          560 t
ORIGIN      
        1 gatggggccg accgtctgtt cccacagtcg cctcaacgag aaactctccg gtttcacttt
       61 gcggctgtcc aagtcctcac gcaacttgtc caccacttcc ttcaaattgt gattttcaag
      121 aagcgccttg aagtcaattc ttttcatgtg ctttacctct cttgatcttc ccaatgacgc
      181 tgaggacaga cagtgctgcc tgtccccaac cgctctttct tttgactacg cattcaacgt
      241 attgatcttc gaaggcagga ttttcaccag caccgaagga tcggaagtgc tgagtttcgc
      301 cttccagcaa accccgatcg gggaggtcgc atcctcgacg cacgtctggt cttcacaggc
      361 gtcagcgctc gcatagatgc ccaccggatc gccgaaatga atcgccgcgg cggacttctg
      421 gttgagttcc cagacaccgg aagtcccgac gcgaatatct tcggtttcac ccaccgctga
      481 ggcgctcatt gcgatgccga caaacgcgtc cgccgcagct tccctgttgg cagcggcgtc
      541 gcccgcgtcc gcgagagagc cggcgggagc cgcgtagtcg ccactcagaa caacaaaatc
      601 cccaatctcg atcactgtgg cgctttccac aggaatcatc gccggctcag gatcgaaata
      661 ccgatgtcga aagttgtttg ccatagcctc atctcctttt tcgagttgat ccatagaact
      721 cctgatccgt cagaccaccc gctcgaaagc ctttctcttg ggattgggtt ctgacggctt
      781 tgctctgttg tgcggtgctt tttctatctt ctatcaatcg gtggatgtgt tcgtcgtcgt
      841 gggcttccag aagtgtttcg ataaaaatgt ccgagaccag ctcaagagga aggttcgcac
      901 accgcagggc ttcctgaaca ttcgcacgtc gggcttgttg tgccgactga tcccgaagtt
      961 catgcagcgt ctctcgaagt ggcctctcca actgctcgat caaatccgga cggcgtgccg
     1021 taagagaagc gacgtccaaa tcgctccaga gaacactctc tttgtcggat gtctctcttt
     1081 tcgtcagaga ctcaaaaagc cctcgagtcg ccgccggatc ggtcaccaga tcgacgctct
     1141 tgatctccgt gatggcctga acgatttcct ccccatcgcg aacgcatgtt ctgccccgag
     1201 cattgatgga catgccaacg gattccggca tggtctcggc aagagagaag accagatccg
     1261 catgctgcgg tagaagccga aggtcagcac gaagtttgcc cccttcaatt cgcggagact
     1321 cataccgccc gaccagatcc cgaacatcac gacagactcc ctgcccagac tgcccctgcg
     1381 tcggatgatt caaataggca cgacatccat cgaaaaatcc caacgcctct tgaatgaccg
     1441 agtcgggata gcggcgaccg ttgcgcgaat tgggtcccag cagacaaaca ttgcgcaaca
     1501 gcaggttctg ccgatccaaa gaagtttcac accaggggga atcgttacac tcaacaagaa
     1561 gcaactccgc ttccaggttc tgattctcca tatctcaaca gacctccttt ctatttcaag
     1621 aatgatcaat cgcggttgcc tgcaggcatt tcgccgcttg ggcaaacaca acctgtcgga
     1681 gatgtgccaa cattccatgg atttcaggaa gagccgagag gagtcatgaa acgtcaatgg
     1741 acgacaatct gttgagaaaa ggattcattg ggggcaaatg gcatacagcc agagctgcgg
     1801 ctggcattcg atcatgtcag tcttagggat gcaagaggat gctctctgga aagtccgcac
     1861 tgttcctctt cacaaagagg gcatcaatgc gtccatttgg gatcaattca aggctgcagc
     1921 cgtcaagggg ttgcttcgac aggaatatct gccggcatca gaggcaggac ttccgccggt
     1981 cctttcagcg cggtttccag ttcatcccac cagtcgtagt tggcaaggta gaccgcgtcg
     2041 aatatcccga ctccattggt cttctgacgt gccatctgaa ccgctcggac aaactgatga
     2101 ggattgtctt tgtactgcag cagatacaat gttccgatca gaggcgcggc attcgagaca
     2161 accttctgcg ccatatccgc tgctccctca accgagtagt aatctttcag gggaaccgat
     2221 tgcgcttcct cgatcgtgac attcgggtag tagagaccga cggcaagcat gtccacaagt
     2281 tccgcgtagc cggtgcggtt gtagtccggc atcatccaag tgtactcggc atggaatttt
     2341 ctggaagccc agtttgctcc cagggacgcg tagtccggat accaggagcc gacatagtcg
     2401 ccaacaaaga ctcccggtcg ggctt
//
LOCUS       DAOQUC010000568         2418 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_53498_length_2418_cov_3.698265, whole genome shotgun sequence.
ACCESSION   DAOQUC010000568 DAOQUC010000000
VERSION     DAOQUC010000568.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2418
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53498_length_2418_cov_3.698265"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="PKH07_10180"
     CDS             <1..224
                     /locus_tag="PKH07_10180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35350.1"
                     /translation="HFEKKREALEAYHSQFVKPERGPEYRMIGVKDYIQTFASRAIHY
                     GSQIGVQYGEAYVVESPIRVMSPERILDI"
     gene            248..1399
                     /gene="bshA"
                     /locus_tag="PKH07_10185"
     CDS             248..1399
                     /gene="bshA"
                     /locus_tag="PKH07_10185"
                     /inference="COORDINATES: protein motif:HMM:TIGR03999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl L-malate synthase
                     BshA"
                     /protein_id="HNT35351.1"
                     /translation="MLRIGIACYPSIGGSGIVATQLGHELCLRGHQVHYFSYDVPVRM
                     LQCECSCEFHHVDVPAYPLMRFPPYAMALASKLYEIACEPGLDVIHVHYAIPHSMSAL
                     LAKQMIQRTTGRTIPFVTTLHGTDTYLVGQEPSYRAVVQHCLQESDAVTAVSEHLRQE
                     TIRIFQLSKPVEVIHNFVECKPLPEHRLKDIGRAETGRTPIHVSNFRSLKRVEDALQV
                     FELVSRACPCCRMVMVGDGPETAAAHKLAEQLGISDQVTFVGRATDVVHYMNSADLLL
                     STSEIESFGLAIAEAMACAMPVVCTRVGGVPEVVEHGKTGFLCDTGDVCGMACAVLQI
                     LTNPSRARSMGIAGRERVEKYFSPDVIVPKYETLYQNLLSSFPSSAEPF"
     gene            complement(1568..>2418)
                     /locus_tag="PKH07_10190"
     CDS             complement(1568..>2418)
                     /locus_tag="PKH07_10190"
                     /inference="COORDINATES: protein motif:HMM:NF014026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HNT35352.1"
                     /translation="SCTLVWQSAEGHLSMGIAHAAELAAKSEVAVVFVGDCISTCGEI
                     RDRAFLDLSGGQDELLRAVYATGTPTVLVHIGGRPLTMEWAFDHVAAALTAWYPGEEG
                     GNAIADVLFGDCTPSGKLPMTWPAYVGQLPLTYDEPMGGRGGHRYIFNSSEPRFVFGY
                     GLSYTTFAYSNLRVPQTVPYGANVPVSVDVTNTGTREGTEVVQVYVRDVLATVKRPKR
                     QLKSFAKVTLKPGETKTVSFELKPESLALWDQQMERVVEPGDFLVMIGGSSKTDLEAG
                     FTVQTP"
BASE COUNT          543 a          660 c          631 g          584 t
ORIGIN      
        1 cgcatttcga gaagaagcgc gaggcattgg aagcctacca ttcgcagttc gtaaagcccg
       61 agcgaggtcc agagtacaga atgatcggcg tcaaggacta cattcagacc tttgcttccc
      121 gagcgattca ctatggttct cagattggtg ttcagtacgg agaggcttac gttgtcgaaa
      181 gcccaattcg ggttatgagt cccgagagga ttctcgatat ttgacctgga tgcattgagg
      241 atttcctatg ctacgtatcg gaattgcctg ttatccctct atcggaggca gtggcattgt
      301 agccacgcaa ttgggtcatg aactctgtct ccgtgggcat caggtacact acttctccta
      361 tgatgttccg gttcgaatgc tgcagtgtga atgctcctgc gaatttcacc atgtagatgt
      421 gcccgcatat cctctgatgc gctttccacc ttacgcgatg gcgctggcat ccaaactcta
      481 tgagatcgcc tgtgagcctg gtcttgatgt catccacgtt cattatgcca taccccattc
      541 catgtccgca cttcttgcca aacagatgat tcagagaacg actggcagga caattccgtt
      601 cgtgacaacg ctgcatggaa ccgacaccta cctcgtcggg caggagcctt cctatcgcgc
      661 tgtagtccag cattgcctcc aggagtcgga cgccgtgaca gccgtttcgg aacacttgag
      721 gcaggaaacc atacgaatct ttcagttgtc gaagcccgta gaggtgattc acaattttgt
      781 tgagtgcaag ccgctgcccg aacaccgact gaaggatatt ggccgtgcag agacgggacg
      841 aacaccgatc cacgtctcca attttcgatc gctcaagaga gtggaagatg cgcttcaggt
      901 ctttgaactg gtgtctcgcg cctgtccctg ttgtcggatg gttatggtgg gggatggacc
      961 tgaaactgcg gcagcccaca aactggcgga gcaacttggg attagcgatc aggtgacttt
     1021 tgtcggacgg gccactgacg ttgtccacta tatgaattca gcggatctgc tgctctccac
     1081 atcggagatc gaatcttttg gactggcgat cgctgaagcg atggcatgtg ccatgcctgt
     1141 tgtctgcacc cgtgttggcg gtgtccccga agtggtcgaa cacggcaaga cgggattcct
     1201 ctgtgatacg ggcgatgttt gcggcatggc ttgcgccgta ctccagatcc taaccaatcc
     1261 gtcaagagcc aggtctatgg gcattgctgg tcgagaacgc gtcgaaaaat acttttctcc
     1321 ggatgttatt gtgcccaaat acgaaaccct ctatcaaaat ctgctgtcgt catttccttc
     1381 gagtgcggaa ccgttctagc ctgacgcatc caaatcaaga gatggtttct gttttgccgt
     1441 gataccatag gacaccagct cagacaacat tctcacatgg gcacaccaca gacgtccaaa
     1501 tatgcaacgg tgccatttgt tgcatccaac ggctcccagt ttgtgagaaa aagtggtttg
     1561 cccacgctca gggcgtttgc actgtaaaac cggcttccag atcggtcttg gaactcccac
     1621 caatcatgac gaggaaatcc cccggctcaa caacccgttc catctgttga tcccaaagag
     1681 ccagcgactc gggcttcagt tcaaaggata cggtcttcgt ctcgcctggt ttgagtgtca
     1741 cctttgcgaa agatttcagc tgccgttttg gcctcttgac tgtggcgaga acgtccctta
     1801 catagacttg taccacttcg gtgccctctc ttgtgcctgt gttggtcaca tcaacagaaa
     1861 cgggaacgtt tgcaccatag ggaacagttt gaggaacccg aagattgctg taagcaaacg
     1921 tggtgtaact cagtccataa ccgaagacaa agcggggctc tgacgagttg aagatgtacc
     1981 tgtggccgcc tcttcctccc atgggctcat cgtaggtaag aggcagctgt ccaacatacg
     2041 caggccatgt cataggcagt tttccgctcg gcgtacagtc gccgaagaga acatccgcaa
     2101 tggcgttgcc gccctcctcg ccgggatacc atgcagtcag cgccgcggca acatggtcaa
     2161 aagcccactc catggtcagt ggacgtccgc cgatatgaac caagaccgtg ggtgtacctg
     2221 ttgcatagac tgctcgcagc aactcatcct gacctccgga gagatcgaga aatgcccgat
     2281 ctcggatctc accacacgtt gaaatgcagt ctccgacaaa cactacggcc acttcgctct
     2341 tggcggccag ctctgcggcg tgggcgattc ccatgctcag atgtccttcg gcgctttgcc
     2401 agacaagggt gcaggagt
//
LOCUS       DAOQUC010000569         2418 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_53508_length_2418_cov_3.059247, whole genome shotgun sequence.
ACCESSION   DAOQUC010000569 DAOQUC010000000
VERSION     DAOQUC010000569.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2418
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53508_length_2418_cov_3.059247"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..481
                     /locus_tag="PKH07_10195"
     CDS             <1..481
                     /locus_tag="PKH07_10195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35353.1"
                     /translation="GTGRAAVYIMGGEVPVLGDLQNAEASDDGNNIFNESLYPNSGYF
                     VWNMSPVTVYAQNNFWSIQYDYGIDQRIYDEEENTGSGRVLFEGYQYDAPNLPFLVAG
                     DADNDGHITQLEMQEIVGSWNTVMGMPGFRKWVDLNEDRFIDSEDIFRMSVLWQTNR"
     gene            720..2384
                     /locus_tag="PKH07_10200"
     CDS             720..2384
                     /locus_tag="PKH07_10200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35354.1"
                     /translation="MARTKLFRVLSIGMGFLFFSFCWIAKGLAQEVTIDTSFEAAEGW
                     ESHIPSSGAWSDTLNNGQTWTGGTLASGGTGTAWIREGAGDATFPAPHAGVRYAVIDY
                     DETGTRGTTYLKTDRVSNPASIRFYVSKSHRFTGKMKMEVSVNGTSWSDTGWQADGWG
                     NATDGNTDLVTVRTWYERTWNINLIGDYYIRWVMHEEGQLPEACFQVDDIRLESLPAT
                     ATPTETPTATDTPTATKTPTSTNTPTFTATATDTPTFTPTTPPTEFWIAPGESIVHVI
                     QTEELNGNIPSTTRPFSIYLSVGRYDDPEALHDSVSGFKYAELREFWNLKGVLEAGKQ
                     WDDKVSSVLENSLLCRGSNTVLDFGILIKENSAYGIAFGGNSICTRNVHITSHPGASM
                     TTGLVSQQDHQNNSAERCVIQNVRTGLWISGVSGLKIRRSRIGQCYYCLVTDGTLNLG
                     RVENDEDPGRNTFQQGVDGLFCLVQSSEIFIPAEGNCWKDPSGNIFTNPDSVYQRMTL
                     SAGGGSEVERTSLAAVESLVDIIPMMCFDPNLPGSGVNSEWWNVLE"
BASE COUNT          568 a          619 c          687 g          544 t
ORIGIN      
        1 gggaaccggt cgcgctgcgg tctacatcat gggtggagag gttcccgttc tgggcgatct
       61 tcagaacgcg gaagcgagcg atgacggaaa caacatattc aatgagtcgt tgtatccgaa
      121 ttccggatac ttcgtctgga atatgtcgcc ggtgaccgtg tacgcacaga acaacttctg
      181 gtctatccag tacgactacg gcattgacca gagaatctac gacgaggagg agaacacggg
      241 ttcaggacgc gttctgtttg agggatatca gtatgatgcg ccgaacctgc cgttcctggt
      301 cgcaggcgat gccgacaacg acggtcacat cactcaactt gagatgcagg agatcgtggg
      361 ttcctggaat acggttatgg ggatgcccgg tttccgcaag tgggtggatc tcaatgaaga
      421 ccggttcatt gactcggaag acatcttccg catgtcggtg ctctggcaga ccaacagata
      481 acaacgcaca gggcaggcct tcgggcctgc cctgatccag cgcaagtggc ttggtaaggg
      541 ggtactgaga atgcttgcac cagacacccg caaggcagtt cagctgtggc tcaagacgca
      601 ccgacagcaa cacaccaaca gcaacgcgct tgattgcatc ctcacatctt ggtaatgtgt
      661 ctgtcgtggt aacgaatggg ttcttcccag tcattctgtt tgtgtggaga cgttttctga
      721 tggcacggac aaaattattc agggttcttt ccatcggcat gggcttcttg tttttctcgt
      781 tctgctggat cgccaagggt cttgcgcagg aggtcactat tgacacaagc ttcgaagccg
      841 cagaagggtg ggagagccat attccctcgt cgggggcatg gtctgacacg ctgaacaacg
      901 gacagacctg gactgggggc actctggcgt caggaggaac tggcacagcc tggattcgtg
      961 agggggcagg tgatgcgaca tttccagccc cacatgcggg agttcgctac gcagtcattg
     1021 actacgatga gacaggaacc cgaggaacta cctatctgaa gacggatcgg gtcagcaatc
     1081 ccgcttcgat tcgcttctat gtctccaaat cgcaccgatt cacgggcaag atgaaaatgg
     1141 aggtttccgt caacgggacg agctggtcgg acaccggctg gcaagccgat ggctggggca
     1201 acgcgacgga tgggaacacc gatctggtga ccgttcgcac ctggtatgag cggacttgga
     1261 acatcaatct catcggagac tactacattc gatgggtgat gcacgaagag ggtcagcttc
     1321 cagaagcgtg ttttcaggtt gacgatattc ggctggagtc tttgccagcc actgcgacgc
     1381 ccactgagac tccgacggca accgatacgc cgaccgcgac gaagaccccc accagcacaa
     1441 acactccgac attcaccgct actgcaaccg atactccgac gttcactcca accactcctc
     1501 caacggagtt ctggatcgcg cccggggaga gcatcgtcca tgtcatccag actgaggaac
     1561 tcaatggcaa tataccaagt accaccagac cgttctcaat ctatctgagc gtcgggcgat
     1621 atgatgatcc ggaagcactt cacgattccg tgtccggttt caaatatgcg gaactgaggg
     1681 agttctggaa tctcaaaggc gttctggaag caggcaagca gtgggatgac aaggtttcat
     1741 ccgtcttgga aaactctctg ctgtgtcgag ggagcaacac cgtcttggat ttcggtattc
     1801 tgatcaagga aaacagcgct tacggcattg ctttcggcgg gaacagcatc tgcacccgga
     1861 atgttcacat cacgtcccac ccgggagcat ctatgaccac tggactggtg tcgcaacagg
     1921 atcatcagaa caattctgct gaacggtgcg tgattcagaa tgtgagaacg ggtctatgga
     1981 tcagtggtgt ttcaggtctc aagattcgac gttcacgaat cggccagtgc tactactgtc
     2041 ttgtcacgga cgggacgctg aatctcggcc gcgttgagaa tgatgaagac cctggacgca
     2101 acacatttca acagggtgtt gatggactct tttgccttgt tcagagcagc gagattttca
     2161 tccctgccga aggtaactgc tggaaagatc catcaggcaa cattttcacc aatccagatt
     2221 ccgtctatca gcgaatgacg ctttcggcgg gtggaggctc tgaagtggag aggacttcac
     2281 ttgcggctgt cgaaagcctt gtagacatca ttccgatgat gtgctttgat ccaaacttgc
     2341 ctggcagcgg cgtcaacagc gaatggtgga acgttcttga gtaaggtttg gctgttgtgg
     2401 cggtgaaggc ttccatct
//
LOCUS       DAOQUC010000570         2417 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_53545_length_2417_cov_2.960627, whole genome shotgun sequence.
ACCESSION   DAOQUC010000570 DAOQUC010000000
VERSION     DAOQUC010000570.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2417
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53545_length_2417_cov_2.960627"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..553)
                     /gene="hypD"
                     /locus_tag="PKH07_10205"
     CDS             complement(<1..553)
                     /gene="hypD"
                     /locus_tag="PKH07_10205"
                     /inference="COORDINATES: protein motif:HMM:TIGR00075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HNT35355.1"
                     /translation="MRFQDLHEAFRQQQLTQRLAHSIRHLLNGQTVRFMEVCGGHTAA
                     VLRFGIQALLPDSIQLLSGPGCPVCVTPQVVVRQSLEVADRDDSLLATFGDMMRVPST
                     DGSLEDARARGASIRVIYSPLDVLDLARENPLKKVVLLAIGFETTAPATAKLLLEAER
                     ANVKNLFVLCAHKTMPEAMSWLLE"
     gene            complement(558..2270)
                     /gene="sppA"
                     /locus_tag="PKH07_10210"
     CDS             complement(558..2270)
                     /gene="sppA"
                     /locus_tag="PKH07_10210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HNT35356.1"
                     /translation="MKRSKRWILLLVLLSIIGVIVIIGSERLATEKAVITPNSVFILD
                     LRESYPEVVTTGIAELMGEPRLSFRQLVTSVRRAAEEPNVRACLLNTGMGGLGWAQTE
                     ELRDALVYFKSTGKPVIAYLETAQTRDYYLASVATEVYMAHEGFLHLSLLAEVPFMNR
                     ALGKLRIEPDFIWEGKYKSSPEMFLRDSMSEPHREEMNSLLDSIKYTMMQSVADVRGI
                     SPEIFQSALDETFLTAKRAKELRLVDQLLYPDEVTQSIKTQLEVTEIGKSYPADYGFT
                     DGLLAKISNRPKVAVVYAVGVIQAGSSQQGGIFGDILGSDTLWRQLEDLRKDSAVRAV
                     VLRIDSPGGSGLASDIIWRGVTRLREKKPVIVSMGDMAASGGYYIAMGADHIIAEPNT
                     LTGSIGVFAGKFNMKGFYDWLGLTFEQLKGGQQADIFSDNSNFSPEQRAIIQSSLSDF
                     YKTFVAKAAQGRKKEFEQIHAVAQGRVWSGIQALDAGLVDQLGGLDTAIDVARERAGI
                     VGDIQLVIYPKKKGILSFFRKGEGKFELQMPEQIQTLMRYEALARMFERDRVLALVPE
                     FIKQ"
BASE COUNT          576 a          696 c          595 g          550 t
ORIGIN      
        1 gttcgagcag ccaggacatg gcctctggca tcgtcttgtg agcacaaaga acaaacaggt
       61 tcttaacatt cgcccgctct gcttccagca gcagtttggc cgtggcaggg gcggtagtct
      121 caaatccaat cgccagcagt accactttct tgagggggtt ctctctcgcc agatcgagca
      181 catcgagtgg cgagtagatc actcggatgg atgcacctct tgcacgagca tcttcaaggc
      241 tgccatcggt ggaaggaacc cgcatcatgt ccccgaatgt cgccaggagc gaatcgtccc
      301 tgtcggcaac ctccaaagac tgtcgaacaa ccacctgagg cgtcacacag accggacatc
      361 ccgggccgga gagcagttgt atgctgtcgg ggagaagcgc ttgaatgcca aatcgaagaa
      421 ccgccgccgt atgcccccca cagacttcca tgaatcgaac ggtctgaccg ttgagcaaat
      481 gacgaatgct gtgcgccaac cgttgcgtca actgctgctg tcgaaaggct tcgtgaagat
      541 cttggaatcg catgaatcta ctgcttgatg aactcgggaa ccagcgcaag aactcggtcg
      601 cgttcaaaca tcctggccag cgcctcgtat ctcatcagtg tctgaatctg ttccggcatc
      661 tggagttcga acttgccctc tcccttgcgg aaaaaggaga ggatcccctt cttctttgga
      721 tagatgacca gttgaatatc cccaacgatg cctgctcgct cccgcgctac atcaatggct
      781 gtgtcaagcc ccccaagttg atccaccaga cccgcgtcca gagcctgaat gccagaccag
      841 actcgtcctt gcgcaaccgc gtggatctgc tcgaattcct tctttctgcc ttgagctgcc
      901 tttgcaacaa aagtcttgta gaaatcagaa agcgacgatt gaatgatggc gcgctgctca
      961 ggactgaaat tgctgttatc actaaagatg tccgcctgct ggccaccctt caactgctca
     1021 aaggtcaaac caagccagtc gtagaagccc ttcatgttga atttcccagc aaagacgccg
     1081 atcgaacctg tcagcgtgtt cggttctgca atgatgtgat cggctcccat ggcaatgtaa
     1141 tagccaccag atgcggccat gtctcccatg gagacgatga cgggcttctt ctccctcaag
     1201 cgtgtaacgc cacgccagat gatgtccgag gcaagccccg atcctcccgg actgtctatc
     1261 cgaagcacca cagcacgaac agctgaatcc ttgcgcaagt cttccaattg tctccagagg
     1321 gtgtcgcttc ccagaatgtc cccaaagatg ccaccctgct gacttgaacc cgcttggatc
     1381 accccgaccg catagacaac tgcaaccttc ggtcggttgg atatctttgc cagcagtcca
     1441 tctgtgaaac cgtaatctgc cggataggat ttgccaatct ctgtcacttc caactgcgtc
     1501 ttgatggatt gggtcacttc gtcggggtac agaagctggt caacaagtct gagttccttc
     1561 gctctcttgg cggtgagaaa cgtctcgtcc agcgcagatt ggaaaatctc aggactgatt
     1621 cctcgaacgt ccgcaacgct ctgcatcatg gtgtatttga tggagtccag cagggagttc
     1681 atctcttcgc ggtgcggttc cgacatggaa tccctcagaa acatttctgg agatgacttg
     1741 tacttgcctt cccagatgaa gtccggttca attcgaagct tccccagcgc cctgttcatg
     1801 aacggaactt cggccagcag gctgagatga agaaaaccct catgagccat atagacctct
     1861 gtcgctacgg acgcaaggta gtagtcacga gtctgggcag tctccagata ggcaatcacg
     1921 ggtttaccgg tactcttgaa atacacaaga gcgtcacgaa gttcttccgt ctgcgcccaa
     1981 cccagaccac ccatgccagt attcagaaga caggctcgga cgttcggctc ctcggccgct
     2041 cgacgaacag aggttaccag ttgtcgaaaa gatagacgtg gctcccccat aagttctgca
     2101 atgccggtcg tcaccacttc gggatagctc tctcgaagat ccaatatgaa cacgctgttg
     2161 ggagtgatta ccgccttctc ggtcgcaaga cgctccgaac cgatgatcac aatcacaccg
     2221 atgatggaca gaagcacaag caggagtatc catcgcttgc ttcgtttcat gatttcatcc
     2281 tccagatact atcgcctcaa tcgaggctcc aaaaaaggat tatcccagaa cgatcaatgt
     2341 ctggcaagtt gtgagaagat aggtgtcgca gcaaagtagt aatgtcccct atgtgcaaag
     2401 taggattgtc ccctttg
//
LOCUS       DAOQUC010000571         2416 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_53582_length_2416_cov_3.132571, whole genome shotgun sequence.
ACCESSION   DAOQUC010000571 DAOQUC010000000
VERSION     DAOQUC010000571.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2416
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53582_length_2416_cov_3.132571"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..863)
                     /locus_tag="PKH07_10215"
     CDS             complement(<1..863)
                     /locus_tag="PKH07_10215"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNT35357.1"
                     /translation="MLSAEEVRRQFAEICDHLDPLSGQKSLLDFSTRLIQQYADLEAD
                     MDSRLEDVTMLHNISRSLMSILDVDDLLRNIVDSVKERLCVASCSLLLLDQNKAQLQI
                     HAASGNRFDNKMLRETRIPVGEGISGTVAQTGEPILVRDIESDPTWGRSNRAGYSSRS
                     LLCVPMKVQDEVIGVLNVNEKKNGTPFDEYDLKILSILAANASAALASARFVNQIRGK
                     ESLISNIAQSVPSGILALGLDGQVLLVNRLLRRFMKIEDSTPTGKSYRSVLDPGVLGM
                     LEPIITRAWETG"
     gene            complement(850..1170)
                     /locus_tag="PKH07_10220"
     CDS             complement(850..1170)
                     /locus_tag="PKH07_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HNT35358.1"
                     /translation="MRQDETKSDQTRKAAALRYRPREDAAPMVVAKGRGLIAERIIAI
                     AKEHEIPIFRDDLLVGLLINQPLDRGIPPTLYEAVAQVLAFLYRLKRKSVARPKGVRE
                     NAVR"
     gene            complement(1219..>2416)
                     /locus_tag="PKH07_10225"
     CDS             complement(1219..>2416)
                     /locus_tag="PKH07_10225"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNT35359.1"
                     /translation="LFSETDKQTIVQTLDRAGLFDKFIAKFWEINSVRQVDLLAMLKY
                     TFMETHVDFLRSCLAHPNTEISSQALLLLAKLKSGLQQGDFAKALGSQNPETVLSAVA
                     ILSEEGTVESLPAIVPLLNHTSEEVARHAALCVARISERQLFSRFDSLGDKVLERIAK
                     TLHRLNPNLISDISAKLDSLSGKEKAKSVRILLSLSTEQEAREALETLMEDENAHVRA
                     SAAKGVSGFSSDEVAGILDKLLQDEDPRVRANAIEEIAGSPNQRVVQMLFQSARSRHG
                     RERANALKKLWELGYRGYELSVAQMIEDPDPYIRASGLWVVGEIEAPSLMKLVQDRLS
                     DPQPIVRRSAVNAIRRKAESNQVRTLFPLLKDPDESVRHAVNMALKERLNLEMAPGHE
                     PEQTGQ"
BASE COUNT          543 a          668 c          620 g          585 t
ORIGIN      
        1 cctgtttccc acgcccgagt gatgatcggc tcgagcattc cgagaacccc agggtccagc
       61 acagatcggt aggatttccc cgtcggggtg ctgtcttcga ttttcatgaa ccggcgcagc
      121 aggcgattca ccagcaatac ctgtccatcc aaacccagtg ccagaattcc actgggaacg
      181 ctctgagcga tgttagagat aagcgattct ttaccccgaa tttggttgac aaaacgtgcg
      241 gatgccaacg cggcggaagc gttcgctgcg agaatgctca aaatcttgag atcatattcg
      301 tcaaacggag ttccattctt tttttcgttg acattgagaa ctccgatgac ttcatcctga
      361 accttcatcg gaacgcagag caacgagcga cttgaatacc ccgcgcggtt gctacgcccc
      421 catgttggat ctgattcgat gtcccggacg agaatgggtt ctccagtctg cgccactgtt
      481 ccgctgatac cttcaccaac ggggatccgc gtttcccgga gcattttatt gtcaaagcga
      541 ttgccactcg cggcatgaat ctggagctga gccttgtttt ggtcgagaag aagcaacgag
      601 cagcttgcca cacagagtct ttccttcacc gagtcaacga tgttgcggag cagatcgtcc
      661 acgtcgagaa tgctcatcag ggagcggctg atattgtgca gcatggtcac atcttcgaga
      721 cgcgaatcca tgtccgcttc cagatcggcg tattgctgaa tcaggcgcgt gctgaaatcc
      781 agaagtgact tctggcctga gagtggatcc aggtggtcac agatttcagc gaattgtcgc
      841 cgaacctctt cagcggacag cattttccct gactcccttc ggtctggcta ccgacttgcg
      901 tttcaaccga tacagaaagg cgagaacctg ggcaacggcc tcgtaaagtg tcgggggaat
      961 gcccctatcc aaaggttgat tgatcagcaa gcccacaagc aggtcatcac gaaagatcgg
     1021 aatctcatgc tctttcgcga tggcgatgat acgttcggcg atcagaccac gacccttggc
     1081 cacaaccatg ggagcagcat cttcgcgcgg ccggtatcgc agtgcggcgg ccttgcgtgt
     1141 ctgatcgctt tttgtctcgt cttgtctcat gtaaacctta gttgaaccgt ctctggggaa
     1201 ggtcacactc cagagactct attgtcctgt ttgctctggc tcatgtcccg gagccatttc
     1261 cagattcagt cgttccttca aggccatgtt gactgcatgt cgaacgcttt catccggatc
     1321 tttcaaaagt ggaaacagag ttcgcacctg gttcgactcg gcctttcttc ggatggcatt
     1381 cacggccgat cttcgaacaa tgggctgcgg atcggacaat cgatcctgca ccagtttcat
     1441 caaacttggt gcttcgatct ctcccacgac ccacagtccg ctggcacgaa tataaggatc
     1501 cggatcctca atcatctgag cgacggacaa ctcatagcct cgatagccca actcccagag
     1561 ttttttcagg gcgttggctc tttctcttcc atgtcggctt cgagcggact gaaacagcat
     1621 ctgaaccacc cgctggttcg gggaaccagc gatctcctca atggcgttgg cacgaactct
     1681 gggatcctcg tcctgaagca gtttgtccag gataccagcg acctcgtcgg acgagaatcc
     1741 agagacacct ttggcagcgg atgcccgaac atgggcgttc tcatcctcca tgagtgtctc
     1801 aagcgcctct cgggcttcct gctccgttga gagagagagc aaaattcgta ccgatttcgc
     1861 cttctccttg cccgacagtg aatctaactt ggcggagatg tcactgatga ggtttggatt
     1921 caaacggtga agcgttttcg cgatgcgttc gagcaccttg tctccaagtg agtcgaaacg
     1981 tgaaaacaac tgcctttcgc tgattcgagc cacgcacagc gctgcatgtc gagccacctc
     2041 ttcggatgtg tggttcaaca gcggaactat ggctggcaat gattcaactg tcccttcctc
     2101 agagaggatg gcaaccgcgc tcaatacggt ctccggattc tgagagccca gcgccttagc
     2161 gaaatcgccc tgttgaaggc cagacttaag ctttgccaac agaagcagag cctgagaact
     2221 aatctccgtg ttgggatgcg caaggcaact tcgcaggaag tctacatggg tctccatgaa
     2281 ggtatacttg agcatggcca agagatcgac ttgtcttacc gagttgattt cccagaactt
     2341 agcgataaat ttgtcgaaaa gtccggcgcg atccaaagtc tgaacaatgg tctgcttgtc
     2401 cgtttccgag aagaga
//
LOCUS       DAOQUC010000572         2416 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_53593_length_2416_cov_2.576027, whole genome shotgun sequence.
ACCESSION   DAOQUC010000572 DAOQUC010000000
VERSION     DAOQUC010000572.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2416
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53593_length_2416_cov_2.576027"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..691)
                     /locus_tag="PKH07_10230"
     CDS             complement(<1..691)
                     /locus_tag="PKH07_10230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35360.1"
                     /translation="MFVRMKTSYFLICFLVLCATAAQGARVPIQNAGFELDLNADGVP
                     DGWNAFADTKKYDASAPERAHSGMASVRLAGNGYWQVISVAPGIPYLYRSWTRGQSGV
                     ENVSHSVDFHGTSGYLETTSQSSVTPSFYRQTGIWFRPIAEANRAALSSRTSRGDWVW
                     QDDVEVFDGFLRNGSLEVKGNGDGVPDCWERKGAPFVIEEEMHRGILGDRHVSARRRE
                     SAGKEVVKHIVL"
     gene            complement(682..1725)
                     /locus_tag="PKH07_10235"
     CDS             complement(682..1725)
                     /locus_tag="PKH07_10235"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HNT35361.1"
                     /translation="MSDSPSRILIVRLSAIGDVIHALPCLHALRAAFPKAHIGWVVEE
                     FAAPLLERMSGLDQLHCLSRGGSKTFRKSGSSKPFLSLVKEVRQAHYDVSVDLQGLTK
                     SAVWGLLSGARMRIGYGDKDGRELSRLLNNRRIVPQANVRHVVDRNLSLLSALGINNP
                     TVRFDLPVHQESAGWARRLMADAGLKPPCVVTHPGAGWQTKRWSPKRYAEVCHRLHDD
                     FGFSVILSTGPREESLCHDFLESLGSVPSAAPAMDQLQLQETIRAADLFIGPDTGPMH
                     LAVALGKPTVAIFGGSDPIRNGPYGSKNLVLTTALSCQPCWRTQCPAVRCLEELTVDE
                     VYPAVKEYWLRCL"
     gene            complement(1722..2204)
                     /locus_tag="PKH07_10240"
     CDS             complement(1722..2204)
                     /locus_tag="PKH07_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002627532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HNT35362.1"
                     /translation="MTTIAKYRTVEQLVEIVREAKAQGKTVVLGNGAFDLLHVGHVRY
                     LQGAKALGDLLIVAVNSDVSVRSIKGSTRPILQEYDRICLVAAIAAVDYVTSFDDTKV
                     SRLLRTLKPDIHAKGTDYRTPEQVPEAAVVAEYGGRTALVGDPKDHSTTDIIGKIRSL
                     "
BASE COUNT          553 a          696 c          642 g          525 t
ORIGIN      
        1 ccagaacgat gtgctttacc acttctttac cggcggactc gcggcgacgg gcggaaacgt
       61 gacggtcacc gaggattccg cgatgcatct cttcctctat cacgaaaggc gcaccttttc
      121 tctcccagca gtctgggacg ccgtcaccat ttcctttcac ctcaagagaa ccgttgcgca
      181 gaaacccatc aaagacttcc acatcgtcct gccaaaccca gtcgccacgg gacgttctcg
      241 aagacagggc tgctctgttt gcctcagcaa tcggacgaaa ccagatacct gtctgacggt
      301 aaaaggatgg agtgacgctg gattggctcg ttgtttccag gtagccggac gtgccatgga
      361 aatccacact atgagagaca ttctcaacgc ccgactgtcc gcgcgtccaa ctgcggtaga
      421 ggtaaggaat tccaggggca acgctgatga cctgccagta gccatttcct gccaatcgaa
      481 cggaagccat tccggagtgt gcacgttccg gcgcgctcgc atcatacttc ttggtgtcgg
      541 cgaacgcatt ccagccgtcc ggaacgccat cggcatttaa gtctagttcg aaacccgcat
      601 tttggatggg aacacgagca ccctgtgccg ctgtcgcaca cagcaccaga aagcagatca
      661 gaaagtacga ggtcttcatc cttacaaaca tcgcaaccaa tactctttga ccgcaggata
      721 gacttcatcc acggtcaact cttccaggca tcgaaccgct ggacattgag ttctccagca
      781 cggctgacag gaaagggctg tggtgagaac cagatttttg gatccgtagg ggccgttgcg
      841 gatggggtcg gaaccaccga atatcgcaac ggtcggcttt cccaaggcaa cggcaaggtg
      901 catcggtcct gtgtcgggac cgataaacag atccgcggcg cgtatcgttt cctgcaactg
      961 caattgatcc atggcagggg ctgcgctcgg aaccgatccg agcgattcaa ggaagtcgtg
     1021 gcacaaggat tcctcacgag gtcccgtgct caggatcaca gagaatccga aatcatcgtg
     1081 aagtcggtgg cagacctcgg catagcgttt gggtgaccag cgcttggtct gccatcccgc
     1141 accaggatgc gttacaacac agggcggctt caagcccgca tcagccatca ggcgtctcgc
     1201 ccaccctgct gattcctgat gaacaggaag gtcaaatcgg acagttggat tgttgatgcc
     1261 aagcgcggaa agcaaggaaa gatttctgtc caccacatgc cggacattgg cctgaggcac
     1321 aatgcgtctg ttgttgagga gtcgtgagag ttccctgccg tccttgtctc catatccgat
     1381 gcgcattcga gcacctgaga gcagtcccca gacggcgctt ttggtcagac cctgaagatc
     1441 aacgctcaca tcatagtgag cctgccggac ttccttgacc agagacagga acggttttga
     1501 cgagccgctc ttgcgaaaag tcttgctgcc accacgactc aggcaatgca gttgatccaa
     1561 tcccgacatt ctttccagaa gaggcgcggc gaattcctca accacccaac cgatatgtgc
     1621 cttgggaaag gcagcacgaa gcgcatgaag acagggcaac gcgtggatca catcgccaat
     1681 ggcgctgagt cgaacgatga gaatccgtga gggtgagtca ctcatagcga ccgtatcttg
     1741 ccgatgatat ccgtggttga atgatccttt ggatccccaa ccaatgccgt tctgccccca
     1801 tactcggcga cgaccgccgc ttcgggaacc tgttcgggcg ttcggtagtc tgttcctttg
     1861 gcgtggatgt ccggtttgag tgttcgaagc agtcgcgaca ccttcgtgtc atcgaaagaa
     1921 gtcacataat ccacagcagc aatggctgcg acgagacaga ttctgtcgta ctcctgcagg
     1981 atcggtcggg tcgagccttt gatgctccgg acagaaacat cgctgttgac tgccacgatg
     2041 agcaaatccc caagagcctt ggctccctgc agatagcgga catgccccac atggagcaga
     2101 tcaaaagcgc cattgccaag gacgacggtc ttgccctggg ctttcgcctc tcgaacaatc
     2161 tcgacgagtt gctcgacggt tctgtacttt gcaatggtgg tcaagtcatc cataccgaat
     2221 tgaggtactc ctttggaatg gaacgcgagg aaaccgactg tgcgatcagt tgccgaagtc
     2281 cattttggta aacaggttta cctgaagatc agcaatgctg ttctccgtca cccgccttcc
     2341 ctcgtattca tattccccct cggtttcgag atagtgcgtc cccttctgct ctgtgcgaag
     2401 gacacgaccc ttcttg
//
LOCUS       DAOQUC010000573         2411 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_53772_length_2411_cov_2.650255, whole genome shotgun sequence.
ACCESSION   DAOQUC010000573 DAOQUC010000000
VERSION     DAOQUC010000573.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53772_length_2411_cov_2.650255"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2411
                     /locus_tag="PKH07_10245"
     CDS             <1..>2411
                     /locus_tag="PKH07_10245"
                     /inference="COORDINATES: protein motif:HMM:NF024972.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35363.1"
                     /translation="GSVQGTFSGQEVNKFLGIRSWGRPIFRVELDYGQSSANETIDNL
                     MFEGEGPTPTETPTQVPMEGCISPGEVYSVPTEGLAVIDLGVLTMTQAKDASGTPIAL
                     SVQDCGYDTLLDAYIPWSEASGTQRAMITLADFCDGMPPQTVRILLHYGSSTLRLNAF
                     NSNGALLDTEYATPGSQEQWVVLHSTDGIRRVRFVGSEICIMRICWTCFGEEPTATDT
                     PTLTPTPPTQESCISAGEVYTSPAFNVPTVDLGVLRFVQAKDAAGVAIPLSVQDCGND
                     TNQDVYIPWSEASGTQRAFLEFTNACAGVAPQVVKILLSYGSPTLRLVGVNSANTIVD
                     TQYAAAGSQEQWVALYSADGVQRVRFVGSEICVIRVCWNCIGERPTATNTPTTTPSFV
                     ASSTPTNTPTNTATSTSTRTPTFTSTFTPSNTPTRTPTSTPTRTPTRTPTRTPTRTPT
                     RTPTRTPTSTPVALDLRFTDHEVVQVIQNSAKNVPLVAGKPTVFRAYVALDPPLASQV
                     GLITGKLYYRRAGVTYGPVFPMNNMSDGIEAVQNPLRKNQKHSLNFRLPKAATLAGTT
                     QFYVELNPTTSLFFYSLLPNAPHLGYVQPMYSAIWSRWLNENDYGNNRYPSAGYVSST
                     FNARTNSFRIGYYSVYYDPPDKAGAAPTSVIHDGDNMFRRIFPVDPDNVDYRYMGQFT
                     FTGNLAQESEIPNIMGQLNLAWCISQLLHAFGAGPQYDQMHAWLPSGAVSYNGCSDPT
                     WYNGGLSRSAFSNATAGALLYESRYRRTFAHEIGHNFGCYHPNDDPSGPWYIDETDCG
                     FDVANG"
BASE COUNT          601 a          689 c          594 g          527 t
ORIGIN      
        1 ggaagtgtgc agggaacctt cagtggccag gaagtcaaca agtttttggg tatccgttcc
       61 tggggtcgtc cgattttccg tgttgaactt gactacgggc agagttccgc caacgagaca
      121 atcgacaatc tgatgtttga aggcgagggt ccaacaccaa cagagacacc gacacaagtc
      181 cccatggaag gctgtatctc accaggagag gtctattcgg ttccgacaga ggggctcgca
      241 gtcatcgatc tgggagtact gacaatgacg caagccaaag atgcctcagg aacaccgatc
      301 gccctaagcg ttcaggactg tggatacgat acgttgcttg atgcctatat tccgtggtca
      361 gaggcttcag gaacgcaacg cgccatgatc acactcgcgg atttctgcga cggaatgcca
      421 ccacagactg tcagaattct gcttcactat ggatcttcaa cactgcgctt gaacgcgttc
      481 aacagcaatg gggctctgct ggatactgag tacgccactc ctggatctca ggagcaatgg
      541 gtggtactgc acagcacaga tggcattcgc cgagttcggt ttgtcggatc tgaaatctgc
      601 atcatgcgga tttgctggac gtgctttgga gaagaaccca ccgccaccga tactcctaca
      661 cttacaccaa caccaccaac acaggagagt tgcatctccg ccggagaggt ctatacatca
      721 ccagccttca acgtgcccac ggtagacctt ggcgtcttga ggtttgtcca ggcaaaggat
      781 gccgccgggg tggcgatccc cctgagcgtt caagactgcg gtaacgacac gaaccaagac
      841 gtgtacattc cctggtcgga agcctccggc acacagagag catttctaga gttcaccaat
      901 gcgtgtgcag gtgttgcgcc acaagtcgtg aagatcctgc tttcatacgg ctcacccaca
      961 ttgcggttag tgggagtcaa tagcgcaaat accatcgtag acacgcagta cgcagcggca
     1021 ggttcacaag aacaatgggt ggccttgtac agtgcagatg gtgttcagcg agtgcgtttt
     1081 gtcggatcgg agatctgtgt tatcagggta tgctggaact gcattggaga gcgacccaca
     1141 gcgacaaata ctcctaccac cacaccctct tttgttgctt caagtactcc aacaaacact
     1201 ccgacaaaca ctgcaaccag tacgtccaca cgcacaccaa cattcacctc gacattcacc
     1261 ccctcgaaca ctccaacacg tacaccaacg tctaccccga cgagaactcc cacgagaaca
     1321 ccgacccgga ctccaacaag aaccccaaca agaacaccaa ctcgaacgcc tacgtcaacg
     1381 ccagtggcac tcgatctccg attcactgac catgaagtgg ttcaggtgat tcagaacagc
     1441 gccaagaacg tgcccctcgt cgctgggaaa ccaactgtgt ttcgtgccta tgttgctctc
     1501 gatcctcctc tcgcttctca ggttgggttg atcactggaa aactgtacta ccgtcgagca
     1561 ggagtcactt acggaccagt cttcccaatg aacaatatgt ccgatggcat agaagccgtt
     1621 cagaatcccc ttcgtaaaaa ccagaagcac tctctgaact tcagacttcc aaaagcggca
     1681 acgctcgccg gaacaactca gttctatgtg gaactgaacc cgacgacttc gttgttcttc
     1741 tacagtcttt taccgaacgc cccacacctt gggtatgtcc agccgatgta ctccgcgatt
     1801 tggtcgcgat ggctcaatga gaatgactac ggcaacaacc gttatccatc tgcaggttat
     1861 gtctcctcga ccttcaacgc ccgcacaaat agcttccgca ttggctatta ctcagtttac
     1921 tacgatccac cggacaaagc gggtgcggct ccgacaagcg taatccatga tggggataac
     1981 atgttccgac ggatattccc cgttgatccg gacaatgtgg actatagata catgggacag
     2041 ttcactttca ccggcaatct ggcacaggag tctgagatac ccaacatcat gggacagttg
     2101 aatctggctt ggtgcatcag ccagctactt catgcattcg gagcagggcc tcagtatgac
     2161 cagatgcacg cgtggctgcc ctccggcgca gttagttaca acggttgttc agacccgaca
     2221 tggtacaatg gtggtctgag tcgttccgct ttcagcaatg cgacggccgg tgctttgctc
     2281 tacgagtcaa gatatcggcg gaccttcgcc cacgagattg ggcacaattt cggatgctac
     2341 catccaaatg atgatccctc gggaccctgg tatatagacg agacggactg cggctttgac
     2401 gtcgccaacg g
//
LOCUS       DAOQUC010000574         2410 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_53805_length_2410_cov_3.267091, whole genome shotgun sequence.
ACCESSION   DAOQUC010000574 DAOQUC010000000
VERSION     DAOQUC010000574.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2410
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53805_length_2410_cov_3.267091"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(147..746)
                     /gene="nadD"
                     /locus_tag="PKH07_10250"
     CDS             complement(147..746)
                     /gene="nadD"
                     /locus_tag="PKH07_10250"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HNT35364.1"
                     /translation="MRVAVFGGTFDPIHVGHLIIAEQARELLSVEQVIFVTASQPPHK
                     RPAECPPEQRLRMVELATQGNPYFQVSQVETNRKGPSYTIDTLRQILSDRSGEIDEMI
                     LLIGGDSLREFDMWKDWREIIQLSSLAVVPRPGVDLAGPPDALARSRILEDIVLTDIS
                     STAIRRRIRKGQSIRYLVHDAVSLYIEQEGLYLREEEKP"
     gene            1039..2199
                     /locus_tag="PKH07_10255"
     CDS             1039..2199
                     /locus_tag="PKH07_10255"
                     /inference="COORDINATES: protein motif:HMM:NF013507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35365.1"
                     /translation="MLKRVFFVCLVTMLSASAAFSAITWTPTSALPAGTKDGALIAYN
                     GYVYLFGGRSAANDVAVSTAFYAPINTDGSLGAWQSTTALPEARALPGAYAYNGRVYV
                     WGGWRADWTTMRTGFYASINPDGSLGSWTTSASLIPQSGGVDQMDSFGRGILGLGKYV
                     YIIGGEMNDASLTNKCYYSEIQGSGDFGTWNETTSLPVPTNGTLKGYWFHGVGIVQGT
                     TSSYIYVVGGNHGGTSESHILSNTINADGSLGTSWTESSTPLLHALYEHGCAVQGNVI
                     YAVGGLNGSTPQTYVQAITVDPTTGTVQGVVDETALAEGRARTHAVCYSAGGKDILLN
                     AGGAVYSGAPYYDTCFYSDITIPTATPTETDTPVPPTPTLSSGVNYDVNRYE"
BASE COUNT          567 a          638 c          621 g          584 t
ORIGIN      
        1 cagaaaccta tctttgtgaa tcggacagaa agcccggatg acgctcccgt gctgttcgac
       61 acgatccaga ttgaagccaa gcatctgaag cgtctgctcc gcattcaaat tgctattgag
      121 acgttcgatc agcatctgca gctgactcac ggcttttctt cctctctcag gtacagaccc
      181 tcttgttcaa tgtacaggct caccgcgtcg tgcaccagat accggatgct ctgacccttg
      241 cggattctcc gacgaatcgc cgttgacgaa atgtccgtca acacaatgtc ctccagaata
      301 cgacttctgg caagagcgtc aggaggtcct gccaaatcca ctcctggcct cggaaccacc
      361 gcaagagaac tcaactggat aatttcgcgc caatccttcc acatgtcaaa ctctcttagc
      421 gaatctcctc caatgagcaa aatcatctca tcaatttcgc cggatcggtc gctcagaatc
      481 tgtcgaaggg tatctatagt ataggaaggc ccttttcgat tcgtctcaac ctgggagact
      541 tgaaagtagg gattgccttg ggtggccagt tccaccatcc gcagacgctg ctcgggtgga
      601 cactccgctg ggcgcttgtg aggcggttga gacgccgtaa caaagatcac ctgttcaacg
      661 ctcaggagtt ccctcgcctg ctcggcaata atcagatgcc cgacatgaat cgggtcaaac
      721 gttcctccga acacagcaac tctcatcttc gtcagttcga ctccttgaat gacagatggt
      781 aacaaagtct cccgctcttg tgcaagtctg gatcaaaaac acccggatca aaaacaaatc
      841 tcgcgttctg cttgacagac ctgatctccg agtcctaaac tggtcgcaaa gtatagcctt
      901 acttgtgttt cagtgaaaga ttcggtggta tggctcgacc gaaaggaggt gagagtccga
      961 gctcgttact tccatagagg ttgaagggtg tttggagact cgatcttcca agtcatagaa
     1021 tgatggaggt ctagagaaat gttaaagaga gttttctttg tttgtctggt gacaatgttg
     1081 tcggcgtcag ctgctttctc ggcaatcact tggacgccca caagtgcact gccggctggt
     1141 accaaggacg gtgcccttat tgcctacaat ggatatgtct atctgttcgg tggaagaagc
     1201 gccgcaaatg acgtagcggt ctcaacggct ttttatgctc cgatcaacac ggacggctca
     1261 ctgggtgcct ggcagtcgac aactgcattg cccgaagcgc gagcccttcc aggtgcctac
     1321 gcctacaacg gtcgagtgta tgtgtggggc ggatggcggg ctgactggac aacgatgcgc
     1381 acgggcttct acgcttcgat caacccagat ggttcgctgg gctcatggac gacctctgca
     1441 tcccttatcc cgcagagcgg aggggtggat cagatggact ctttcggtcg aggcattctc
     1501 ggactgggaa agtatgtcta catcattggc ggtgagatga acgacgcaag tctcaccaac
     1561 aaatgctact attccgagat ccagggtagc ggagacttcg gaacgtggaa tgaaacaacc
     1621 agtctgcccg tgccaaccaa tgggactctc aaaggctatt ggttccatgg agttggcatc
     1681 gttcaaggaa ccactagcag ttacatatac gtcgtcggtg gcaaccatgg cggaacaagt
     1741 gagtcccaca ttctttccaa tactataaat gcagatggga gtctggggac gtcatggaca
     1801 gagtcatcaa cccctctgtt gcatgctctt tacgaacatg gttgcgccgt ccaaggcaat
     1861 gttatctacg cggtaggtgg tttgaacggt tccacacccc agacttacgt tcaggcgata
     1921 acagttgatc ccactaccgg cacggttcag ggagttgtgg atgagactgc gctcgctgaa
     1981 ggtcgtgcac gaacccatgc tgtgtgctat tcggctggcg ggaaggatat tctgctgaat
     2041 gcaggtggcg cggtctactc aggagcaccc tactacgata cctgtttcta ctcagacatc
     2101 accattccga cagcaacacc gaccgaaaca gatacaccag tgccaccaac acctacactg
     2161 tcaagcggtg tcaactacga cgtcaacaga tacgagtgac aaagtttaga gtgatcctct
     2221 ggggaactgc aggagcggag gtctattctc cgctcctcat ttcccgtggg atggtctctt
     2281 tttgttttgg cgtagtagat ccacgtggct ggtgagctct ctttcctgcg tggcagcctt
     2341 caactcattg agggctactc tttgatgtga cacaggcatt cgtgcgtgtg attttcagtg
     2401 ctgagacaca
//
LOCUS       DAOQUC010000575         2409 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_53842_length_2409_cov_2.935429, whole genome shotgun sequence.
ACCESSION   DAOQUC010000575 DAOQUC010000000
VERSION     DAOQUC010000575.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2409)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2409)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2409
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53842_length_2409_cov_2.935429"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            517..1260
                     /locus_tag="PKH07_10260"
     CDS             517..1260
                     /locus_tag="PKH07_10260"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HNT35366.1"
                     /translation="MLFIAHRGASYDAPENTLASINLAWKVNAKAVEIDVYLTADENI
                     VAIHDKTAERTGGKDLLIREQTLDALRQLDFGRHKGEQWAGERIPTFPEILATVPEDG
                     KMFVEIKCGSEIIFKLRECVQRSGLRTDQIVYICFDLPTLEAVKRALPEHLCYWLRSI
                     KKNPETGVWEPDVESMIEGASKAGLDGLDVQAREIVDSSFVKKVRGAGMGIYVWTVNE
                     IPLAQAMVEAGVDGITTDRPHWLRESLGL"
     gene            1455..>2409
                     /locus_tag="PKH07_10265"
     CDS             1455..>2409
                     /locus_tag="PKH07_10265"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HNT35367.1"
                     /translation="MPGRDSLSPRILCGGRQKQRYVLISGNGNADCVGGITTKVNLPL
                     GKTYCLRVQFKMTRDVNPLLNLLYEISHGGVSQQIVEFHRLSDSRAVGEAMVVTSDGQ
                     GSVSAEIRLIYHLCAKGKIWIEKVSLTETDTMPPRKVRVACTGGPDSLEEYGLPTFQK
                     ALDAVGRKKVDLVLLPEYFHGEYVQEPLSGPSATLMSQKAKEYAMYVAGTIGLHDEAK
                     DRVYNAALLYDRQGNLIGRYDKFHLYGPELHSDGITPGESVPVFQTHFGRVGFMTCYD
                     SWFADVAELLALRGADIILFPSLGFDRALMHARSLDNCIYIV"
BASE COUNT          585 a          551 c          653 g          620 t
ORIGIN      
        1 ctctgtctgc tattctttgt catacggata aacctccctg gattgaattc acaagtttct
       61 taaatgtatg gcaatctcta tgccaaatct ttgctcattc gagattcaat gacattatct
      121 attgatgata aaggagatat gaaggaagtg aattgctctc ccagaagtcc agtgattcag
      181 agaaactaca ttgccgtagg atagcaatat gctagccacc atcgtgtcgg tctgattaga
      241 aatgtcgtcc atcgtttctg ctgcggcttc ctctgacgta gaaggatcgt gccaggtatt
      301 cagcgacagg tacaactgaa cacttggcca tacaacctct gaaggtgtgc agaagcccag
      361 gatgagtaca ttggcgatct cctcgactgc tttgtcgcga gcacatgtcg tctatgcctc
      421 ttgcacattc cacggccgaa agatagactc tgtgcaagtg aaccatattg gacgaaactt
      481 gcagaactat ctgcgcagac ccaaggagac cgtcttatgc tgtttattgc tcatcgtgga
      541 gcgtcttatg acgctccgga gaacacgctt gcctcgatca accttgcatg gaaagtgaat
      601 gccaaggcgg ttgagataga cgtctatctg actgctgatg aaaacatcgt tgcgatacac
      661 gacaagactg ctgagcggac aggtggcaag gatttgctca tccgtgagca gaccctcgat
      721 gcactgagac agttggactt tggcagacac aagggagaac aatgggcggg ggagaggatt
      781 cccacatttc ccgaaatcct tgcaactgtg cctgaagatg gaaagatgtt tgtcgagatc
      841 aagtgcggtt ctgagataat tttcaaactt cgggagtgtg tacagcggtc tggtcttcgg
      901 actgaccaga ttgtctacat ctgctttgat ctgccaactt tggaagcagt caagagagca
      961 cttcctgagc atctctgcta ttggcttcga agcatcaaaa agaatccgga aaccggtgta
     1021 tgggagcctg atgttgagtc tatgattgag ggtgcgagca aggctgggct tgacggtctg
     1081 gatgttcagg cacgcgagat tgttgatagt tctttcgtga agaaagttcg aggcgcgggc
     1141 atggggatct acgtctggac agtcaacgag atcccactcg cccaggcaat ggtcgaagcc
     1201 ggcgtggacg ggattactac cgatcgtcct cattggctcc gcgaaagtct aggtctgtag
     1261 agcgacatca ttggagtttg gggcttggag taagactggt gtctactgtc tcaaaatctc
     1321 tctgttattc tcaaacgtta ctctggctat gcatggtggc aagcggaatt gcctgggcgc
     1381 aggaaacgaa tctggttcca aacggatctt ttcagtgccc aattgtcaac ggactcccag
     1441 agggctggga gaaagtgcct ggtagagatt ctctttcgcc ccggatactg tgcggtggca
     1501 gacagaagca acgatacgtt ctgatttcag gcaatggcaa tgccgactgt gttggaggaa
     1561 tcacaacaaa ggtaaatctc cctctgggaa agacctattg tctccgtgtt caattcaaaa
     1621 tgactcgtga tgtcaaccca ctattaaacc tcttgtatga gatctcccat ggtggtgttt
     1681 cacaacagat cgttgagttt cacagactca gtgacagtcg agcagtggga gaggcgatgg
     1741 ttgtcaccag cgatgggcaa gggagtgttt cggcagagat tcgccttatc tatcaccttt
     1801 gcgccaaggg gaagatatgg attgaaaaag tatcgctcac tgagactgat acgatgccgc
     1861 ctcgaaaggt tcgggttgcc tgcacaggtg gtccagacag ccttgaagaa tatgggcttc
     1921 cgacatttca gaaagctctg gacgctgtcg gacggaagaa ggttgacctt gtccttcttc
     1981 ctgagtactt ccatggtgaa tacgttcagg agccactctc aggcccgtcg gcaaccctga
     2041 tgtctcagaa ggctaaagag tacgcgatgt atgtggccgg aactattggt ctccacgacg
     2101 aagcgaaaga ccgtgtctac aatgccgcgc tgctctacga ccggcagggg aatctcattg
     2161 gtcgttatga taagtttcac ctctatggtc cggagttgca ctcggatggt atcactccag
     2221 gagagagtgt tcctgtgttc cagactcatt ttggaagagt tggtttcatg acgtgctatg
     2281 acagttggtt tgcagacgtt gctgaactgc tggcgttgcg cggggcagac atcatcctgt
     2341 tcccgagttt gggatttgac cgcgctctga tgcacgcacg ttcgctggat aactgcatct
     2401 acatcgttg
//
LOCUS       DAOQUC010000576         2409 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_53856_length_2409_cov_2.432031, whole genome shotgun sequence.
ACCESSION   DAOQUC010000576 DAOQUC010000000
VERSION     DAOQUC010000576.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2409)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2409)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2409
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53856_length_2409_cov_2.432031"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1789)
                     /locus_tag="PKH07_10270"
     CDS             complement(<1..1789)
                     /locus_tag="PKH07_10270"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNT35368.1"
                     /translation="MAKFEVGENVAGFLIESEIGRGGMGVVYKARQISLQRTVAIKRI
                     PESCSADADSLGRFKREALATARLNHPGIVQIYDYCDVDGESFYVMEYLEPKEWTSLQ
                     KILDEEILLESNRALMLCRGILSGLKVAHEAGLVHRDIKPANIMVNKSGEVRILDFGI
                     AICAEMTRLTMDQCTPSGTLYYMSPEQGAGGDIDGRTDIYALGTMLYQMLAGVLPFRA
                     QDPMALLRMRSDTDAAPLRKANPDVSEDVESIVMKALARDPAQRYATAQEMADDIELF
                     LKKQPFMVSDVTRDEPKSQECDEDAEVFILEINVEESRLRVGYGTQGKEIPTVSRYDH
                     AQVTEKEMAEWSAKCFSIIYEANKTSSPNPKYLAELKSLSQSMYERLLPLSVKTALQA
                     TSAKQMLLRLDDKAIQIPWELLWDGKEFLCLRFRCGRMVSTFKAVFDQPARTVGDTTR
                     FLILSNPKGDLKASTEECRILKDQIAGFSHVNVRVRNARISVNQTREDIRDADIVHYS
                     GHARYDTLNPEGSGWLLNDGVFGATNVRQLQGGHSTLPILVFANACRSATFDANDTKS
                     APGRVFSLANAFLLAGVQNYIGTFSEVLDA"
     gene            complement(1776..>2409)
                     /locus_tag="PKH07_10275"
     CDS             complement(1776..>2409)
                     /locus_tag="PKH07_10275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35369.1"
                     /translation="FEGMRSDAPCFLHFRGGVKGEIALHFEQVTFDMNDLLALATNLV
                     SEGRFADAIGIFKERAHVGKDKDVFERLLDVLAGGTSILGELVPVTAGSSLRSGSPSE
                     ATSDSTRVLTEELLGRWSSQLPLISRSVEECREILNVLRQAKKDRALDGRVDLVCRVL
                     EVWSRVDEKEREVEDVLFAMAAELKAIVDEQGDTSCTAEQEGEGISNGEI"
BASE COUNT          563 a          721 c          556 g          569 t
ORIGIN      
        1 gagcgtccaa gacctcagaa aaagtgccaa tgtaattctg aacacctgcg agcaggaatg
       61 cgttggccag actgaagact ctgcctggag cgctcttggt gtcgttcgca tcgaacgttg
      121 cagaccggca cgcgttggca aaaacaagga tcgggagcgt cgaatggcca ccctgcaact
      181 gacgtacgtt tgtggcacca aagacaccat cattgagcag ccatccagag ccttctggat
      241 tcagagtatc gtaccgcgca tgtccgctgt aatgtacaat gtctgcgtcg cggatgtctt
      301 cacgagtctg gttcactgag atgcgcgcat ttcgaacacg aacattcacg tgagagaaac
      361 ccgcgatctg atccttgaga atacggcatt cctctgttga cgccttgaga tcgcctttgg
      421 gattgctcag aatgagaaag cgcgttgtat caccaaccgt ccgggcaggt tgatcgaaga
      481 ctgctttgaa ggtgctgacc atccggccac aacgaaaacg cagacagaga aactccttgc
      541 cgtcccagag cagttcccat ggaatctgta tcgccttgtc atccagcctc agaagcatct
      601 gcttggcgct ggtggcttgc agagcagtct ttaccgacag aggcaacagc ctctcgtaca
      661 tgctctggct caggctcttc aactcagcaa gatacttcgg attgggggat gaagtcttgt
      721 ttgcctcata gatgattgag aaacactttg cactccattc ggccatttcc ttctcagtta
      781 cctgggcatg gtcatagcgg cttactgtgg gaatctcttt accctgagtc ccatagccga
      841 cacgcaaccg ggattcctca acgttgatct ccaggatgaa tacctcagca tcttcatcac
      901 actcttgtga cttcggttcg tcccgcgtga cgtcagatac cataaaaggc tgcttcttca
      961 gaaacagttc aatatcgtcc gccatctcct gagcggtcgc atacctctga gctggatcgc
     1021 gcgccaatgc cttcatgaca atactctcga catcttccga aacatccgga tttgcctttc
     1081 tcagtggcgc tgcatcagta tctgaccgca tgcgaagcag tgccatcgga tcctgcgccc
     1141 ggaaaggaag tacacccgca agcatctgat agagcatcgt gccaagtgcg tagatgtccg
     1201 tgcgcccatc aatgtcgccc ccggcaccct gctccggcga catgtagtac agggtgccgg
     1261 aaggagtgca ttgatccatc gtgagccgcg tcatctcggc gcaaatcgcg atcccgaagt
     1321 caagaatcct gacctctccg gatttgttta ccatgatgtt ggcaggcttt atgtcccgat
     1381 gaacaagacc ggcctcgtgc gccactttga gtcccgagag gattcctctg catagcatca
     1441 gcgcccgatt cgattcaagg agaatctcct cgtcgagaat cttctgcagt gatgtccatt
     1501 ccttgggttc cagatattcc atgacataga acgattctcc atccacatcg cagtagtcgt
     1561 atatctggac gattcccgga tgattcaggc gcgccgtcgc cagcgcttct ctcttgaatc
     1621 gtccaagtga atccgcatcg gcagaacagc tctccggaat tctcttgatg gcaacggttc
     1681 tttgcagaga tatctgacgc gccttgtaga cgacacccat accccctcgc ccgatctcag
     1741 actcgataag aaagccggca acattttcac ccacttcaaa tttcgccatt tgagataccc
     1801 tcaccttcct gttcagccgt gcatgacgta tcgccctgtt catcaacaat tgccttcaac
     1861 tcagccgcca tcgcaaacaa tacgtcttcc acttctcttt ctttctcatc cactcgtgac
     1921 cacacttcaa gcactctgca caccagatca accctcccat caagcgccct gtccttcttt
     1981 gcctgcctga ggacgttgag aatctcgcga cactcttcca cactccttga gatcaacggc
     2041 agttgagatg accagcgccc cagcagttcc tctgtcagga ctcgggtgct gtcactggtc
     2101 gcttcagaag gggagccact gcgaagagag gatccagcag tcactggaac caactcaccc
     2161 aggatggacg ttccacctgc aagcacatca aggagacgct caaatacatc cttgtccttc
     2221 ccaacgtgag ccctctcttt gaaaatgccg atcgcgtcag caaatctacc ttctgaaacc
     2281 aggttcgtgg ccagagccag aaggtcgttc atgtcaaagg tgacctgctc aaaatgcagt
     2341 gctatctcgc ctttcactcc acctcggaaa tgcagaaaac agggagcatc gctcctcatc
     2401 ccctcaaaa
//
LOCUS       DAOQUC010000577         2408 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_53900_length_2408_cov_2.502762, whole genome shotgun sequence.
ACCESSION   DAOQUC010000577 DAOQUC010000000
VERSION     DAOQUC010000577.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2408)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53900_length_2408_cov_2.502762"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..531)
                     /locus_tag="PKH07_10280"
     CDS             complement(<1..531)
                     /locus_tag="PKH07_10280"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNT35370.1"
                     /translation="MAVKLAIHGGPRTIEVGRIQPWPPIDDTDRQFVLKSLESHRPSF
                     GPNCEALQEEFATWNGNRFAIATNSGTAALHMGVAACGCGAGDEVIVPAYSWSSSVTC
                     VLHHNAIPIFVDIDWETMNLDVDRIEEAITPRTKAILVVHLHGLVAPMDKILAIAEKH
                     GLKVIEDACQAHGARYK"
     gene            complement(769..>2408)
                     /locus_tag="PKH07_10285"
     CDS             complement(769..>2408)
                     /locus_tag="PKH07_10285"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HNT35371.1"
                     /translation="DQYAVGKIRLEGNTLFGEDEIRQAMLTDRGQVFNQSQFVKDLET
                     VRDLYRDQGYILAQIQPRLDKDEQNDVVDVTLSISEGYLIFLGNVIVQGVTTFDDGTR
                     EAIGLKTRDHVILREIRMSWDERIAWKKVGSDPSVYEQKDDVAQEREEPYVLDWGEVK
                     KSKRRLENLGFFKKDRVEFEPQLTEDPEMMDLLVRLEEQHTGNISFGAGFSTEYGASL
                     FFGLSEKNLFGRGQALDLSGEIGSRRQSFRIGFTEPYLFDSDYSFGADLYNTTIERFS
                     GRDFQENRKGGSIRIGRQFWEHYTWYVRLKHEAVKLDDVFYGGGRVVTEEMPPEYEEL
                     STKVNSLTLTLVKDTRDLFQAPTKGYKNLVSLEQAGFGGTPFTKVKAETSWYTKLTQK
                     MVLAVNGELGAIGEFSDEIPLSERFFLGGAETVRGYEYGTISPVDFISYWRDDHGLYL
                     DRRDVRVGGEEMMLLKTEVRYPITDIIEGVVFVDAGGTWRELGDFDTGVIRYSTGLGL
                     RIQLPIGAMIRLDYGHALNPDEYQDTQPIHFSFGHSF"
BASE COUNT          540 a          702 c          609 g          557 t
ORIGIN      
        1 tttgtaacgg gctccatgag cctgacaagc atcctcgatt accttcagtc cgtgcttctc
       61 ggcgatggcg agaatcttgt ccataggcgc aacaaggccg tggagatgca caaccagtat
      121 tgcttttgtc ctgggtgtaa tggcctcttc gatccgatcc acgtccaggt tcatcgtctc
      181 ccagtcaata tcaacgaata tgggaattgc gttgtgatga agaacacaag ttaccgagga
      241 ggaccaggag taagcgggaa cgatgacttc atcccctgca ccgcatccgc aagcagcaac
      301 gcccatgtga agagccgcag tccccgaatt ggtcgcgatg gcaaaccgat tgccattcca
      361 agtggcgaac tcttcctgaa gtgcttcgca gttggggccg aaacttggac ggtgggattc
      421 cagagacttg aggacgaatt gtcgatctgt gtcatcaatg ggtggccaag gctggatgcg
      481 tccgacctca atggttctgg gtcctccgtg gatagcgagt ttcactgcca tggcgaggct
      541 cctttcgttc ctgaggatac atcccgtgca tgacccatcc cattcctgag aaaggacgct
      601 ccagaacaca aacatcccag tgacactcag tccaaggaga gttgtcgttc acagagcagg
      661 cgtcaataga ttggtcttat tccgctcaac cgcaggtgct gagttgctca gcaggtgcag
      721 atctgctcag tgcgaacggt catgtcggca accttcatgc acctggttct agaacgaatg
      781 cccaaacgag aaatggatag gctgtgtgtc ctggtactcg tccgggttca gcgcgtgacc
      841 atagtccaaa cggatcattg ctccgatggg cagctgaatt cgaagaccca agcccgtgct
      901 gtaacggatg actccggtgt cgaagtcgcc aagttcacgc caggttccgc ctgcgtccac
      961 aaacacaacc ccttcgatga tatcggtgat gggatagcgc acttccgtct tcagaagcat
     1021 catctcctca ccgccgacgc gcacatcacg gcgatcgagg tagaggccat ggtcatcacg
     1081 ccagtaactg atgaagtcta ccggactgat tgtgccgtac tcatagccac gaacggtctc
     1141 tgcgccaccc aggaagaaac gttccgaaag cgggatctcg tcgctgaact ccccaatcgc
     1201 tcccaactca ccattgacgg caagcaccat cttctgggtg agctttgtgt accacgaagt
     1261 ctcggccttg acctttgtga acggagttcc gccgaagccc gcctgttcca gcgacacgag
     1321 attcttgtag cccttcgtcg gtgcctggaa taaatcgcga gtgtctttga ccaacgtcag
     1381 agtcagactg ttgaccttgg tggagagttc ctcatactcg ggaggcattt cctccgtgac
     1441 cacacgcccg ccgccgtaga agacgtcatc cagtttgacc gcttcgtgtt tcaggcggac
     1501 ataccacgtg tagtgctccc agaactgtct gccgatgcga atggatccac ccttgcgatt
     1561 ctcctggaaa tcacgtccac tgaatcgctc aatcgttgtg ttgtagagat ccgcaccaaa
     1621 ggagtaatcg ctgtcgaaca aataaggttc tgtgaagcca attcggaacg actgccgtcg
     1681 gctgccgatc tctccgctga gatccagcgc ctgtccgcga ccaaagaggt tcttctctga
     1741 caggccgaag aacagagacg ccccgtattc agtgctgaat ccggctccga atgagatgtt
     1801 gccggtatgc tgctcctcaa gccgtaccag caagtccatc atctcgggat cttcagtcaa
     1861 ctgaggctca aactcaactc ggtctttctt gaagaaaccc aagttctcca accgtctctt
     1921 gctcttcttt acttcgcccc agtccaagac ataaggctct tcgcgctcct gcgcgacgtc
     1981 gtctttctgc tcgtatacgg aaggatcgct tccaaccttc ttccaagcga tccgctcatc
     2041 ccacgacatg cgaatctcgc gcaggatgac gtgatcgcgg gtcttcagac cgatggcttc
     2101 gcgagtgccg tcatcgaatg tggttacgcc ctggacgatg acgttgccca gaaagatcaa
     2161 gtatccctca ctgatggaga gcgtgacatc aacgacgtcg ttctgctcat ccttgtccag
     2221 tcggggctga atctgtgcaa gaatgtaacc ttgatcccga tacaaatccc gcaccgtttc
     2281 aagatccttg acgaactgcg actggttgaa tacctgcccg cggtcggtca gcatcgcttg
     2341 ccggatttca tcctctccaa agagggtgtt tccctccaaa cgaatcttgc caacagcgta
     2401 ctgatctc
//
LOCUS       DAOQUC010000578         2405 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_54004_length_2405_cov_2.659574, whole genome shotgun sequence.
ACCESSION   DAOQUC010000578 DAOQUC010000000
VERSION     DAOQUC010000578.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2405)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54004_length_2405_cov_2.659574"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            263..1219
                     /locus_tag="PKH07_10290"
     CDS             263..1219
                     /locus_tag="PKH07_10290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35372.1"
                     /translation="MRSWVTFILIGFLVCLALNEEALCQQTSSKVLAKIPWGEKDNQA
                     GLLSRPEMERCGPLSFCRSGLDEFLLLDTVKARVVQVEKGGAISTLATDVLGWSICAV
                     GGQGFFVLGEKGLSQFGFLGEPRALFALDADRKIIQGYGTEVMLLRIPNAVASTVAIA
                     NVDQMVYPIAGGTVGMEMKTLTGEQARAQLGKSDYDGMIYQVKRINSRDVRVLGLDSG
                     GKELVTIPIQSGSDALGAVLFKCDDGKGGVFVELERLVGQKAELEVHHYARTGELLCL
                     HRLPNDYFTTVYKKTEIGADGEVIQMLTRKDGVLFIQYGEEH"
     gene            1221..>2405
                     /locus_tag="PKH07_10295"
     CDS             1221..>2405
                     /locus_tag="PKH07_10295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HNT35373.1"
                     /translation="MTIACKRSVLMLCFVFGLCLPAWTVNYPYEGSVSSNVKRDRILA
                     IAEDYATLYYYQSAANIDYSGYTGATCPDATVGWKTGMKYCWGGEDSTREYLLRMTEG
                     DGAGNKDTSSSSSYDQYCAGADCSGFVSNAWTSSRVATSGFPNISDDILWEQLRQGDA
                     LNDAGSHIRLFDYFISQVGTAMLYESTAGGGAWKCIHRSLARDDTYQPIRYRAATYRV
                     VVYPQPDITYIKRTGVERVEVRWDGEANTGFNLYLSLDGTTWTRIRDSVALTYLDRVC
                     EVSGLLPDHTYYFKMTSQNTAGESVDSDVVAYRLDGALTHRLLLVDGSDRYNDQNSGA
                     SHTLLTRFGDALAQCGIGFDFCSNEAVVDEQIDLRSYSAVVWMLGEESTFDETFSWAE
                     QMH"
BASE COUNT          527 a          603 c          720 g          555 t
ORIGIN      
        1 ccttctcctg tgggagaagg tcgcgatgag ggcaagcgga cgcgtcgctg caaggtttgt
       61 aatgtgcccg taagggcata ctccgggtca ccccttacgg cgtaactacg aactgacaag
      121 agatcacgcg atgcatctcc acaacgaagt tgaccctgtg cgtcggtctg tgtaagactt
      181 gcacctgaga tggtgtggag cgtctgtgct catctgaaat gggttttgtt gatgctgctg
      241 acaacagata aggagaactg ccatgcgttc ttgggttacc ttcatcttga taggcttcct
      301 tgtgtgtctc gcgctcaatg aagaggcgct atgccaacag acttcgtcca aggtgctcgc
      361 gaaaatcccc tggggcgaga aagacaatca agccggcctt ctgagccgac cggagatgga
      421 gcgatgcggg ccactgagtt tttgtcggag cggtctggac gaatttctgc ttctggatac
      481 cgtcaaggcg cgggttgtcc aagttgaaaa gggcggcgca atctcgactc ttgcgacaga
      541 cgttcttggg tggtctatct gtgcagtcgg tggccaaggc ttctttgtcc ttggtgagaa
      601 gggactctcg cagttcggct ttctgggtga gccgagagcg ctcttcgcgt tggacgccga
      661 ccggaagatc atccaaggct atgggacgga ggtcatgctc ctgcgcattc ccaatgccgt
      721 tgccagtaca gttgcgatcg ccaatgttga ccagatggtc tatccgatcg ctggaggaac
      781 tgtgggcatg gagatgaaaa ccctgaccgg tgagcaggcg cgggcgcaat tggggaagag
      841 cgactacgac ggcatgattt accaggtgaa gcgcatcaac agtcgagatg ttcgcgtgct
      901 gggacttgac tcgggtggca aggaactggt gacgattccc attcagtcgg gatctgacgc
      961 actcggggca gtgctgttca aatgcgacga tggcaaggga ggcgtgtttg tcgagttgga
     1021 gcgtctggtt ggtcagaagg ccgagctgga agtacatcac tatgcgcgaa cgggtgaact
     1081 tctctgtctc catcgtcttc caaacgatta cttcacgact gtctacaaga agactgagat
     1141 tggcgccgac ggtgaggtga ttcagatgct cacccgcaaa gacggcgttc tctttattca
     1201 atatggggag gagcactgaa atgaccattg catgcaagag aagtgtgctc atgctgtgtt
     1261 ttgtgtttgg actatgcctt ccggcgtgga cagtgaacta tccctatgag ggttcagtca
     1321 gcagcaatgt caaacgggat cggattcttg ccatcgctga agactatgcg acactgtatt
     1381 actatcaaag cgctgcaaac atagactact caggctacac tggagcaacc tgccccgacg
     1441 cgactgtcgg ctggaagacc ggcatgaaat actgctgggg aggcgaggac agcacccggg
     1501 aatacctgct ccgcatgacc gaaggcgacg gcgcgggaaa caaggacacc agttcctcat
     1561 catcctatga ccaatactgt gcgggtgcgg actgctcagg atttgtgtcc aacgcctgga
     1621 caagttcccg agtcgcgact tcgggattcc cgaatatctc ggatgacatt ctctgggagc
     1681 aactgcgtca gggcgatgca ctcaacgatg cgggcagcca cattcgtctc tttgattact
     1741 tcatctcgca agtgggaaca gcgatgctct atgaatccac tgccggcggg ggtgcgtgga
     1801 aatgcattca tcgttctctg gctcgcgacg acacctatca gccgatacgg tatcgcgccg
     1861 cgacgtaccg cgttgtcgtc tatccacagc cggacattac ctacatcaag cgaaccggtg
     1921 tcgaacgggt cgaagtgcga tgggatggcg aagcaaacac gggcttcaat ctctatctga
     1981 gcctcgacgg aacaacatgg acgcgcattc gcgactcggt agccctgacg tatctggatc
     2041 gggtctgtga ggtttcggga ctgttgcccg atcacacgta ctatttcaag atgacatctc
     2101 agaatactgc tggggaatcg gtggactcgg atgttgttgc ctatcggctg gatggtgcgc
     2161 tgacgcatcg gctcctgttg gtggatggca gtgaccgcta caacgatcag aactccggcg
     2221 cttcacatac actgctgacg cggtttggtg atgcactggc gcaatgcggc atcggttttg
     2281 atttctgcag caatgaagcc gttgtagatg agcagatcga tcttcgcagc tacagcgccg
     2341 ttgtttggat gctgggcgaa gagtccacat ttgatgagac cttttcctgg gcagaacaaa
     2401 tgcac
//
LOCUS       DAOQUC010000579         2404 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_54024_length_2404_cov_3.326096, whole genome shotgun sequence.
ACCESSION   DAOQUC010000579 DAOQUC010000000
VERSION     DAOQUC010000579.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2404
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54024_length_2404_cov_3.326096"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..488)
                     /locus_tag="PKH07_10300"
     CDS             complement(<1..488)
                     /locus_tag="PKH07_10300"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HNT35374.1"
                     /translation="MLAIGAHPDDAELGCGGLLASLADKGHRVGIIDLTRGEMGSSGS
                     PEIRAEEARRAGAILGIDFRLNLDMGDGQIVDSQENRLKLAQAFREYKPTVVATSHPI
                     DRHPDHSAATALVKAAALAARLPKMPLNGSIHCVTRILLYCIHDVATPSFIVDISSHF
                     EK"
     gene            complement(517..>2404)
                     /locus_tag="PKH07_10305"
     CDS             complement(517..>2404)
                     /locus_tag="PKH07_10305"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HNT35375.1"
                     /translation="SMRRKRLGDLLVENGMISRSQLETIIGEVSSRGSRLGENLVERG
                     LATEEGIGQALATQLSMPYYPLDSEELDPKVVRLIPRALASKFGMLAVELEGSVLVVA
                     MIDPLDVRAFDTLRAQLGYQIRPAVAPKKAILKCIQTYYDMAGVLDETISKATSEQAA
                     RSLEVEDILDETEAEGGDVSEVGAPVVTMVNTLLQRAVESEASDIHIEPEEDETHVRF
                     RNDGTLRHILNIPKNLHDAVVSRVKIMSRLDISERRRPQDGRAKMRILGRPIDFRISV
                     LPTLLGEKVVIRILDKEQAVRKMDDLGMLQTERAPLEQALQRTRGIILVTGPTGSGKT
                     TTLYAALQSIREESRNLLTIEDPVEYRLQGVNQIQINPKAGLTFASGLRSMLRQDPDI
                     ILVGEIRDRETAEIAIQAALTGHLVLSTLHTNDAPSAVTRLMDMGIEPFLISATVTCA
                     IAQRLIRKICNSCKTEIEPPADVLNRLGIRSLGGRKVYQGTGCVRCRATGYSGRTAIF
                     EVMSIDQKLREIIAARGTRSELSRAASKNGMRSLLVSGLEKVFAGISTLEEVLRLSED
                     KPEDLAYCPGCLESVDPDFLVCPHCAHQLKQPAASCHQCHREVRPEWKACPYCGTPRE
                     TEVS"
BASE COUNT          514 a          678 c          658 g          554 t
ORIGIN      
        1 ttcttctcga aatgcgaaga aatgtcaacg atgaatgaag gtgtggccac gtcatgaatg
       61 caatatagaa gaatgcgtgt gacacaatgg atactgccgt tcaggggcat cttgggcagc
      121 ctcgccgcga gtgccgccgc cttgaccagt gctgtggcgg cgctgtgatc cggatgtcgg
      181 tcaatcggat gggacgtggc aaccacagtc ggtttgtatt cccgaaatgc ctgtgcgagt
      241 ttgagacggt tttcctggga gtctacgatc tggccgtctc ccatatctag gttcaaacgg
      301 aaatcaatgc caagaatagc accggcacga cgggcttcct cagctcgaat ctcggggctt
      361 ccactggagc ccatctcacc ccgcgtcaga tcgatgatcc cgacacgatg tcccttgtcc
      421 gccagtgagg ccaagagacc accgcagccc agctcggcgt catcaggatg ggcgccaatg
      481 gccagcaaag ttgtttcctt ccgattggaa atcaaatcag gagacctctg tttcgcgcgg
      541 cgttccacag taaggacagg ccttccattc gggtcgaact tcccgatggc attggtgaca
      601 ggacgcagca ggctgcttca actgatgcgc gcaatgagga cagaccagga agtcgggatc
      661 cactgattca aggcaaccgg gacagtacgc aagatcctcc ggcttgtctt ccgacagccg
      721 aaggacttct tcaagcgtgc tgattccggc aaaaactttc tccagacccg aaaccagcag
      781 acttcgcatt ccgttcttgc ttgccgctcg ggacaactca cttcgtgtgc cacgagcagc
      841 aatgatctca cggagtttct gatctatgga catcacttca aagatggccg tccgcccgga
      901 gtagccggtt gcgcgacatc gaacacatcc ggtgccctgg tacacctttc tgcccccaag
      961 agagcgaatc ccaagtctgt tgagcacatc tgccgggggc tctatctctg tcttgcagct
     1021 gttgcagatc ttgcgaatca gccgctgtgc gatggcacag gtgaccgttg cagaaatcag
     1081 aaagggttcg atgcccatat ccatcagtcg agtgacggcg gatggagcgt cattggtgtg
     1141 aagtgttgag agcacaagat gtccggtcaa ggccgcctga atggctatct cggcggtttc
     1201 tctgtcgcgg atctcaccga ccagaatgat gtccggatcc tgacggagca tagaacgcag
     1261 acccgaggca aaagtgagtc ccgccttggg attgatctga atctggttca ctccctgaag
     1321 acggtactcc acagggtctt cgatggtcag aagatttctc gactcctcgc gaatactctg
     1381 gagcgcagcg tagagcgttg ttgtctttcc gctgccggtg ggtccggtca ccaggatgat
     1441 ccctcgtgtt cgttgaagag cttgttccag tggtgctcgt tccgtctgga gcatcccaag
     1501 atcatccatc tttcggacgg cctgctcctt gtcaagaatt cggatgacca ccttttcccc
     1561 aaggagcgtg ggaagtacgg aaatgcggaa gtctatgggt cggccgagaa tccgcatctt
     1621 ggcgcgacca tcctgtggac gacgacgttc tgagatatcg agtcgggaca taatcttgac
     1681 acgcgatacg accgcatcat gcagattctt cgggatgttc agaatatgcc gcagagtgcc
     1741 gtcattgcgg aatcggacgt gcgtctcatc ttcttcgggt tcgatgtgaa tatcactggc
     1801 ctcggattcc accgcacgct gaaggagggt gttgaccatc gttaccactg gagcgccgac
     1861 ctcggataca tcgcctcctt cagcctccgt ttcatcgagg atgtcctcga cttccaggga
     1921 gcgagccgcc tgttcacttg tggccttcga tatggtttcg tccagcacgc ccgccatgtc
     1981 atagtatgtc tggatgcact tcaagattgc tttcttgggg gcgactgccg gtcggatttg
     2041 atagcccagc tgtgcgcgaa gcgtgtcaaa ggctcggacg tccagcgggt caatcatcgc
     2101 gacaacaaga acgctcccct ccagctcaac ggccagcatg ccgaacttgc tcgccagcgc
     2161 tcttgggatc aaacgaacca ccttcggatc caattcttca ctgtcaaggg ggtagtaggg
     2221 catggacaac tgggtggcaa gtgcttggcc aattccttcc tccgtcgcca gaccgcgttc
     2281 gacaagattc tctcccagtc gagagcctct cgaggatacc tctccaatga tcgtctccag
     2341 ttgtgaacgg gagatcattc cgttttcgac aagaagatca cccaaacgtt ttcttcgcat
     2401 actt
//
LOCUS       DAOQUC010000580         2404 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_54028_length_2404_cov_2.873563, whole genome shotgun sequence.
ACCESSION   DAOQUC010000580 DAOQUC010000000
VERSION     DAOQUC010000580.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2404
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54028_length_2404_cov_2.873563"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2404)
                     /locus_tag="PKH07_10310"
     CDS             complement(<1..>2404)
                     /locus_tag="PKH07_10310"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HNT35376.1"
                     /translation="QRGQLFIGPWYSAPDCNCISGESVVRNLLMGHRTASRFGKPMKV
                     GYTPFGWGQQAQLPQIYQGFGIDTAFFYRGISAEDAPRSEYLWESPDGTRVLASRFGC
                     AARYNFYMFIWRPVLYKGRTIGSHLPDRLYYWDEKGVPFKFCDAEHRYDHYFLNAPRN
                     FYDPESLEKYFRKLMEAEKQHFRTAAIPLMQGMDTSMPDPLELELIEKIRAFLGADEE
                     IKISSMPDYVKALKCFLKGQDLPVLKGEMRSPGGASSQTSLTGDIISTRPRQKRLTEK
                     AERMLQRWAEPFSTIAWTLGEEYPERYLEIAWKNLLQCQAHDTIGGCGVDQLERDATY
                     RLNQVVNISNTLMRSALGSLQTRINSSLAKDDEVVLTVFNPSPFPRTEVVEAFVDVPK
                     QTGFIHVHIFDEQGNEIDLYEGRRRDTEKVVRSNVDVTTALLCNELSLQFLAESVPAL
                     GYRTYVLKGGGHVPLKKAIATSSHEMENEFLRVEFDDDGTLSVTHKATGKTYEDLHRF
                     EDGGEAGDPWQWKPPAKDRIISSIGCPVQISLEENTHLSATIHVRYQMMIPDSLGHDT
                     SYHDSWRSETEKPLVIDSFFSLRKGSSFLEVETHLDNQHKNHRLRVHFPTRLKAKTAS
                     AETSFAVVDREIERGPNSPFRFTYNPTQPTLRFVSLTDGKDGLTLFSDGQREYEAGDD
                     EERTVALTLIRAFEITLCTVTNRWERLPEMELSHAPGKHVFRYAIYPHAGNWDSADVM
                     RTVESFSLPMLSAQTTPCKGQDLPSVKSFLELTPKQLQLSAVKKAERGKDLVVRIYNP
                     TG"
BASE COUNT          522 a          666 c          640 g          576 t
ORIGIN      
        1 ttgcccgtcg gattgtagat tcggacaacg agatccttgc cacgttcggc tttcttgaca
       61 gcgctcagtt gcagttgttt gggcgtgagt tcgagaaacg acttcaccga cggcaagtcc
      121 tgacccttgc agggtgtcgt ctgagcggac agcatcggca gcgagaagga ctcaacggtt
      181 ctcatcacgt ctgcactgtc ccagttgccc gcatgaggat agatggcata ccggaacacg
      241 tgcttcccag gagcatggct caactccatc tccggaagac gctcccaacg attcgtgaca
      301 gtacagagcg tgatctcaaa ggcgcggatc aacgtcagag ccactgtgcg ctcttcgtca
      361 tctcccgcct cgtattccct ctgtccgtcg ctgaacaggg taagcccgtc cttcccgtcg
      421 gttagagaga caaagcggag cgtcggctga gtcggattgt atgtgaaacg gaacggactg
      481 ttcggtcccc gctcgatctc cctgtccaca acggcgaaac tagtctctgc agaagcggtc
      541 tttgccttca gacgtgtcgg gaaatgcaca cgcagccgat ggtttttgtg ctgattgtcc
      601 agatgcgttt ccacttccag gaaagaactc ccttttcgaa gggagaagaa ggaatctatg
      661 accagcggct tttccgtctc actgcgccag gagtcgtgat agctggtatc gtgtcccagg
      721 ctgtcaggaa tcatcatctg atatcggaca tgaatggttg ctgacagatg cgtgttttct
      781 tccagtgaaa tctgaacggg acatccaata gaactgatga tcctgtcttt ggcaggtggc
      841 ttccattgcc acggatcccc tgcttctcca ccatcttcaa agcggtgcag atcttcgtat
      901 gttttgccag ttgccttgtg tgtcacgctg agagtcccgt catcgtcaaa ctcaaccctg
      961 aggaattcat tttccatctc atgggatgac gtggcgatcg ctttcttcag cggaacatga
     1021 ccgccaccct tcaggacata ggttcggtat ccaagtgcgg gcaccgattc agcaagaaac
     1081 tgaaggctga gttcattgca cagcagcgca gttgtcacat ccacattgct tcggacgacc
     1141 ttctcggtgt cgcgccgcct gccttcgtac aggtcaattt cattgccctg ctcgtcgaaa
     1201 atatgaacat ggatgaagcc tgtctgctta ggaacatcca caaaggcctc aaccacttct
     1261 gtccgaggga acggtgatgg gttgaagacc gtcaagacaa cctcgtcgtc cttggcgaga
     1321 ctcgaattga tgcgtgtctg gagacttccc agagccgatc gcatcagagt attggaaatg
     1381 ttcaccactt ggttcagacg atacgtagca tcgcgttcga gttgatccac gccacacccg
     1441 ccgatcgtgt cgtgcgcctg gcattgaaga aggttcttcc aggcaatctc gagatacctc
     1501 tctgggtact cttcaccgag tgtccaggcg atggttgaga acggctctgc ccagcgctgc
     1561 agcattcttt ctgctttctc ggtgagccgt ttctgacgcg gccgcgtgga gatgatgtct
     1621 cccgtaagtg aagtttgaga actagcgcca cccgggctgc gcatctcacc cttcagcaca
     1681 ggcaagtcct gtcccttgag aaaacacttc agcgctttga cgtagtcggg catcgaactg
     1741 atcttgatct cttcatctgc accaagaaaa gcgcgaatct tttcgatcaa ctccagctcc
     1801 agcggatcgg gcatcgaagt gtccatgccc tgcatcaggg gaattgcggc ggttcggaaa
     1861 tgttgcttct ccgcttccat gagtttgcgg aagtacttct ccagactctc gggatcgtag
     1921 aaattcctcg gggcgttgag gaaatagtga tcgtatcggt gttccgcatc gcagaacttg
     1981 aagggaaccc ccttctcgtc ccagtaatag aggcggtcgg ggagatggct gccaatggtt
     2041 ctccctttat agagcacggg tcgccagatg aacatgtaga agttgtagcg tgctgcgcaa
     2101 ccgaatcggg aagccagcac gcgcgttccg tccggtgact cccaaagata ttccgaacgt
     2161 ggagcatcct ctgcggaaat cccacggtag aaaaaggccg tgtcaatgcc gaatccctga
     2221 tatatctgag ggagctgcgc ctgttgtccc cacccgaatg gggtgtaccc aaccttcatg
     2281 ggtttcccaa accgcgatgc cgttcgatgt cccatcaaaa ggttacgaac gacggattct
     2341 ccggagatgc agttgcagtc gggagcgctg taccacggtc caatgaacaa ctgtccccgc
     2401 tgaa
//
LOCUS       DAOQUC010000581         2403 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_54061_length_2403_cov_2.912692, whole genome shotgun sequence.
ACCESSION   DAOQUC010000581 DAOQUC010000000
VERSION     DAOQUC010000581.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2403
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54061_length_2403_cov_2.912692"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..748)
                     /locus_tag="PKH07_10315"
     CDS             complement(<1..748)
                     /locus_tag="PKH07_10315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35377.1"
                     /translation="MTRSKRVRPGANWPGQRSRETTPSCEEPRDDFFFVLGHQPLLSL
                     AELANVLRTSGANIEQYRLMGDVVVIRSGHSLDCPKLLGILGGSVKAGRVLAHLSEPN
                     PETLWESLISDSVLGGLAESAKPVTLAISLYSSLPEEQFPMAQRRSVIEETNKRCKKF
                     LAEKGVSCRYLIWEGSRRAEPIASAQLIKTHTLESGAEICLVWDEKGLFIGKTEAVQE
                     LDAFAKRDMHKPVRRTTEGLLPPKLARILVN"
     gene            complement(745..>2403)
                     /locus_tag="PKH07_10320"
     CDS             complement(745..>2403)
                     /locus_tag="PKH07_10320"
                     /inference="COORDINATES: protein motif:HMM:NF014628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HNT35378.1"
                     /translation="ETIKPDIGSVVACARANLRSVLLSAQMGITGANVAVAENGSLVI
                     VENEGNARLLASLPSIHVVIIGMDKLVECSEDAMAVLRMLTRCASGDRITSYTTWIRG
                     PIPYTEGGVEHRELHIILLDNGRSQWAQDPWYQEVFQCIQCGACENVCPPNRRVGGQV
                     FGDSYPGPIGLVLAGLLGPQERSDRLLDLCHQCGACKEVCPGKVDIPSLILQLKQEHV
                     ERFGLPWRSRFMLQKVLPRRRVFNGLMKIGSVIQRPLVDRDEFIRSVPMFRDQTRFRS
                     LSALARKTFRDLWAQLAPRSIEKPVATVTLFVGCGVNFIYPEIGEAVVRVLRASGVQV
                     VFPTRQTCCGSPAHFAGDVQTLFSLARENVQALCASPSDCIITVCPSCAVALKHTYPA
                     LAPDDETWQRRAQEIADKTKDFSEFLVDLLNFVPETRMRSETGDRERVTYHEPCHLAR
                     TLGVRGQPKRLIRQLEGAELVEMGDPGTCCGFNAGFSFSFPELSSEILKEKLENIEAT
                     DAEFVLTDCPGCIFQIRGGLNRRGSRIETMHTAEFLAWKMGLLR"
BASE COUNT          564 a          690 c          629 g          520 t
ORIGIN      
        1 gattgaccag aatgcgtgcc agttttggtg gcaggagtcc ttccgtagtt ctccgtacag
       61 gtttgtgcat gtcacgcttg gcgaaggcat ccaattcctg aaccgcttcc gtcttgccga
      121 tgaacagtcc cttctcgtcc caaacgagac agatttccgc accgctctcc aaggtgtgtg
      181 tcttgatcag ttgtgcagac gcgattggct ccgcacgcct ggatccttcc cagatcaggt
      241 accgacaaga aacacccttt tcggcaagaa attttttgca gcgcttgttg gtctcctcga
      301 tgacagatcg gcgttgcgcc atggggaact gttcttcggg gagactgctg tacaagctga
      361 tcgccaaagt gaccggcttg gcgctttcgg caaggccgcc cagaacggaa tctgagatga
      421 gtgattccca tagagtctca ggatttggtt cggagaggtg agcaagaaca cgacctgctt
      481 tgactgagcc tcccagaatg ccgagaagct tggggcagtc cagcgagtgg ccacttcgaa
      541 tcacgacgac atcacccatc aaacgatact gttcgatgtt tgcccctgaa gttcgcaaaa
      601 cgttcgccag ttctgccaaa gacagcagag gctggtggcc aagcacaaag aagaaatcgt
      661 cccgtggttc ctcacaggat ggagtcgttt ctctggaacg ctgtccaggc cagttagctc
      721 cggggcgaac tctcttggaa cgtgtcatcg gaggagcccc atcttccacg caaggaattc
      781 cgcagtgtgc attgtttcaa tccgtgaccc tcgacggttc agaccaccgc ggatctggaa
      841 aatgcatccg ggacaatccg tcaggacaaa ctcggcatcg gttgcttcaa tgttctcgag
      901 tttctctttg agaatctccg aagagagttc aggaaaggag aaggaaaagc cagcattgaa
      961 tccacaacac gttcccggat cgcccatttc caccaattcc gcaccttcca gttggcgaat
     1021 cagcctcttg ggctgccctc gaacgcccag agttcgagca aggtgacagg gttcgtgata
     1081 ggtcacccgc tctcggtccc ccgtctcgct acgcattctg gtttcgggaa cgaaattcaa
     1141 gagatcaaca aggaactcag agaagtcctt cgtcttatcg gcgatttcct gagcccggcg
     1201 ctgccatgtc tcgtcatcag gtgcgagtgc gggataggtg tgcttgagtg cgacggcaca
     1261 agatggacac accgtaatga tgcagtccga aggtgaggca cacaacgctt gcacattctc
     1321 acgggcaagg ctgaaaagtg tctgtacgtc cccggcaaag tgcgccgggg aaccgcaaca
     1381 agtctgccgt gttggaaaga ccacctgaac accactggca cggagtacac gcaccaccgc
     1441 ctctccgatc tcggggtaga tgaagttcac tccgcagcca acaaacagcg tcaccgttgc
     1501 gactggtttc tctatggagc gaggcgccag ttgcgcccag agatctcgga acgtttttcg
     1561 ggccagtgct gaaaggcttc gaaagcgtgt ctgatcacgg aacatgggca cgcttcggat
     1621 aaattcatct cgatcaacaa gaggtctttg aatcaccgac ccgatcttca tcaaaccatt
     1681 gaagacgcgc cgtcggggga gcaccttctg aagcatgaat cgggatctcc atggaagccc
     1741 aaatcgctcg acgtgctcct gcttcaactg aagaatcagg gaaggaatat ccacctttcc
     1801 cgggcaaact tccttgcagg ctccacattg gtggcacaaa tccaacagtc tgtctgatcg
     1861 ctcttggggt ccgagcagac ccgccaagac caggcctata gggcctgggt aggagtctcc
     1921 gaaaacctga ccgccaactc ttctgttggg cggacagaca ttctcgcagg caccgcattg
     1981 aatgcactga aagacttcct gataccaggg atcctgtgcc cactgcgatc gtccgttgtc
     2041 cagaaggatg atgtgcaact cccgatgttc caccccgccc tcggtgtaag gaatcgggcc
     2101 tcgaatccag gttgtgtatg acgtgattcg atctcccgaa gcacagcgcg tcagcatccg
     2161 aagcacggcc atggcatctt cggaacactc caccagtttg tccatgccaa taatcacaac
     2221 atggatggaa ggcagtgagg caagcagtcg cgcattgcct tcattctcga cgataaccaa
     2281 tgagccgttc tccgccacag caacattggc accagtgatt cccatctgtg ccgacaaaag
     2341 cacactgcgc agattcgctc tcgcacacgc aaccaccgag ccaatatcag gcttgattgt
     2401 ctc
//
LOCUS       DAOQUC010000582         2393 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_54441_length_2393_cov_2.209153, whole genome shotgun sequence.
ACCESSION   DAOQUC010000582 DAOQUC010000000
VERSION     DAOQUC010000582.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2393
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54441_length_2393_cov_2.209153"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..803)
                     /gene="alr"
                     /locus_tag="PKH07_10325"
     CDS             complement(<1..803)
                     /gene="alr"
                     /locus_tag="PKH07_10325"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HNT35379.1"
                     /translation="MERFRPTVAVIDQAAFVHNLRLVRKRIGDCRQLMAVVKANAYGH
                     GAVALAKAAADSVADWLGVATVEEALELRESGILSPILVFGAILPSQGCEAIQNQVRL
                     VVPSIEIAESINSVARAMGVRAKVHVKVDTGMGRIGFWYEDIGDIFERLCSLEHLEIE
                     GIMTHFPESDSAHKSFTHWQIGRFGEIRSLLESKGVRIPIYHAANSGAVLDHPGSYFD
                     MVRPGIMLYGQHPSEECSREEALRAVMSLKTQIVFLKTVPAGRSISYART"
     gene            complement(806..2149)
                     /locus_tag="PKH07_10330"
     CDS             complement(806..2149)
                     /locus_tag="PKH07_10330"
                     /inference="COORDINATES: protein motif:HMM:NF015587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HNT35380.1"
                     /translation="MKVIRCLKVDPVAVRRWLQRIHSVLGQDFGELDRLSHSKAESWL
                     LKTAKIVQLALVGFVRDQCALRASALTFGTLFAIVPMLAFAFSVTKGLEFQGDLRIVI
                     ISRVPEVFQEIVEKIFLYVENTNAKALGSFGLVFLVVTVVKTLTAAESTFNLIWSVKM
                     ARPMYRKFADYVSVIVVAPVLALTAIGLQSAGAIQRALEIGVVSRVWETFLPLTIIWA
                     CFTFVYMFLPNTKVRFHAAAVAGVFTGFCWLLAQWAYLRFQVDLTKANKIYGGFAAVP
                     FFIMWVYIAWLVTLLGVELSFAIQNARFFVPTWAQRGMTPREEEAAALTVMKAISDHF
                     RAGRGVLSAEQLASSCGLPFRLVSVVLQYLADGGNVVRTSGDTVGYLPAVPSEILTVK
                     RVLDAVRNHGKASQVLDSNFDEYSQGIERDLDALLESRFGKMRLDTTCKNDSPGK"
     gene            complement(2156..>2393)
                     /locus_tag="PKH07_10335"
     CDS             complement(2156..>2393)
                     /locus_tag="PKH07_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005898391.1"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HNT35381.1"
                     /translation="GSLRFRVNSYHHQCADRVADGFRVTARADDGIVEAMEHEHLPYM
                     VCVQFHPELLTDRPEFLRLFQLHVQACLDGRASR"
BASE COUNT          643 a          691 c          555 g          504 t
ORIGIN      
        1 gttctggcgt agctgatgct tcgccccgct ggaaccgtct tcaagaaaac aatctgcgtt
       61 ttgagactca tcactgcgcg aagagcctct tcccgagagc attcctctga aggatgctga
      121 ccgtatagca tgatgccggg acgcaccata tcgaagtagg atcccggatg gtcgagaacc
      181 gcaccgctat tggcagcatg gtagattggg atgcgcaccc ccttgctctc gagaagtgag
      241 cgaatttctc caaaacgccc tatctgccaa tgtgtgaagg acttgtgagc agaatctgac
      301 tctggaaaat gagtcatgat tccctcaatc tccaagtgct ccaaactgca aagtctttca
      361 aagatgtctc cgatatcctc ataccagaat ccgattctcc ccattccggt gtccaccttg
      421 acgtgtacct ttgcccgaac gcccattgcg cgcgctacgg agttgatgga ttctgcaatc
      481 tcaatactcg ggacgaccaa tcgaacttga ttctgaattg cttcacagcc ttgggatgga
      541 aggatcgccc caaagacgag aatgggagat aaaatgcccg actcccgaag ttcaagcgct
      601 tcttcaaccg tcgccactcc gagccagtcc gccacactgt ctgccgctgc cttggcaagg
      661 gcaacagccc cgtgaccgta cgcattagcc ttgacaacgg ccatcaactg cctgcaatcg
      721 ccaatccttt tgcggacaag acgaagattg tgaacaaacg cggcctgatc aatgacggca
      781 acagtcggtc gaaatctttc catgctcatt tcccggggga gtcattcttg caggttgtat
      841 caagacgcat cttcccaaat cgagactcaa ggagagcatc caggtctcgt tcgattcctt
      901 gcgagtattc atcgaagttc gagtcaagaa cttgcgacgc cttaccgtgg tttctcacgg
      961 catccaaaac cctcttcaca gtcaatatct cagatggaac tgcaggcaga tagccgacag
     1021 tgtctccact cgtgcggaca acattccctc catccgcgag atactgcaga acaacggata
     1081 ccaaccgaaa gggaagtccg caagatgatg ccaactgctc cgcggacaac actccccttc
     1141 ccgctctgaa atggtcgctg atggccttca tgacagtcaa agcggcggct tcctcttctc
     1201 tgggagtcat cccgcgttga gcccaagttg gaacaaagaa cctcgcgttc tgaatggcaa
     1261 aggagagttc gactcccaat agagtgacca accaggcaat atagacccac atgataaaga
     1321 atggtacggc cgcaaaacca ccgtagatct tgtttgcttt cgtgaggtca acttggaatc
     1381 gaagatacgc ccactgagcc aataaccagc aaaaacctgt aaacactccg gcaactgccg
     1441 cagcgtggaa gcgcaccttg gtgttcggaa gaaacatgta aacaaacgtg aaacacgccc
     1501 agatgatggt caacggaaga aacgtctccc acacgcggct gaccacacca atctccaaag
     1561 cccgctgaat ggcacccgct gactgaagcc caattgccgt caaagcaagc actggcgcca
     1621 cgacgatcac actcacatag tcggcaaact tccggtacat tggtcgggcc atcttcaccg
     1681 accagatcag gttgaaggtg gactctgcag cagtcagcgt cttgacaacg gtgacaacta
     1741 agaaaaccag gccgaatgaa cccaaagcct ttgcattggt gttctcaaca tagagaaata
     1801 tcttctctac gatctcctga aagacttccg gtactctaga gatgataaca attctcaagt
     1861 ccccttggaa ctccagaccc ttggtaactg agaacgcaaa cgccaacatt ggaacgatgg
     1921 caaagagtgt tccaaaagtg agtgccgacg ctctcaaggc gcattgatct cgtacaaaac
     1981 ccaccaacgc cagctgaaca atcttcgcgg tcttgagcag ccaggactct gccttggaat
     2041 gggagaggcg atccagttcg ccgaagtcct gtccgagcac ggagtgaatt cgctgaagcc
     2101 atcttctgac tgcaaccgga tccactttca aacatcgaat aaccttcact ggaatctacc
     2161 tcgaggctct cccatccaag caggcttgga cgtgcaactg gaataacctg agaaactccg
     2221 gcctgtctgt aagcaactca ggatggaatt gtacacaaac catgtatgga agatgctcat
     2281 gctccattgc ttcaacaata ccatcgtcgg cacgagctgt cacacgaaaa ccatccgcga
     2341 cacgatccgc acactgatgg tggtaactgt tgacccgaaa cctgaggctc ccg
//
LOCUS       DAOQUC010000583         2390 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_54522_length_2390_cov_3.395717, whole genome shotgun sequence.
ACCESSION   DAOQUC010000583 DAOQUC010000000
VERSION     DAOQUC010000583.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2390
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54522_length_2390_cov_3.395717"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..454
                     /locus_tag="PKH07_10340"
     CDS             <1..454
                     /locus_tag="PKH07_10340"
                     /inference="COORDINATES: protein motif:HMM:NF024768.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="electron transport complex protein RnfC"
                     /protein_id="HNT35382.1"
                     /translation="DFPAFEGVQYCCECGVCELYSCPMEISPRRIIQNLKKTAKVDRK
                     KVDEPAVSDLRETRKVPTKRLEARLALTDYHKPAPWKDVAIVPKSVCIALKSHVGVAS
                     EPMVSVGDSVRRGQKIARIPEGKLGANIHASIDGRVTEIANGVVRIQQ"
     gene            451..1305
                     /locus_tag="PKH07_10345"
     CDS             451..1305
                     /locus_tag="PKH07_10345"
                     /inference="COORDINATES: protein motif:HMM:NF023385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNT35383.1"
                     /translation="MNCQPVVLTVDVEDYFMSPESIAFEQWSSFEDRVQVGLNRLLDM
                     LSTCGRTATFFFLGYVAERHPEAVISVAKAGHEVAVHHYWHQPYEQIGKARFLEGLRR
                     TKGILEDLIGTKVIGHRAPMWSVRRDMPWVFEGLLENGFQYDSSLFPIRTYLYGDAGA
                     EPEPHWLNFGGGRILEVPPSTQTVFGRRIPVGGGFFLRAYPLGMTRWGINRRLRSGRS
                     VVLYVHPWELDPGHPRLQLPTKLAWIHAVGLNRLGRKMTELVKQYEFTSLGNALSRFE
                     AIAGGTNA"
     gene            1298..1846
                     /locus_tag="PKH07_10350"
     CDS             1298..1846
                     /locus_tag="PKH07_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005815271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HNT35384.1"
                     /translation="MPEAVGVVEWRSIAIAIEATDAMLDAAEVEVLVSTPVCSGKYVT
                     IVSGDVASVQVAVETGVREGGNSLVEAVVIPNVHPDIQPAITATTGVENVRSLGVIEV
                     FSVAGAVYAADASVKSADVRLIEIRCARGLGGKAFVTLTGDVDAVRTATETGAAIARE
                     KGLLAASVVIPDLNEKLRRFVL"
     gene            1968..>2390
                     /locus_tag="PKH07_10355"
     CDS             1968..>2390
                     /locus_tag="PKH07_10355"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNT35385.1"
                     /translation="MDSAPPVALSLVIPVYNEKDAVAETLVRACSVIEQSGISAEIIV
                     VDDGSDDGTGDVLKNFGSSVQIVRHPVNRGYGAAIKTGLRHAQGEWIAITDADGTYPL
                     EKLPELWNLTQDAEMVVGMRPRSAQPMIRRPAKWFLARL"
BASE COUNT          529 a          586 c          728 g          547 t
ORIGIN      
        1 agactttccc gccttcgagg gggttcagta ttgttgtgaa tgcggagtct gtgagttgta
       61 ttcctgtccg atggaaatct ccccgcgtcg catcattcag aacctgaaga agaccgcaaa
      121 agtggatcgc aagaaggttg acgagcctgc tgtcagcgac ctgcgtgaaa cgagaaaggt
      181 gccaacaaaa cgactggaag cacgcttggc tctgaccgac tatcacaaac ctgcaccatg
      241 gaaagatgtg gccattgtgc cgaaatcggt ctgcatcgct ctgaaatctc acgtcggagt
      301 cgcttcggaa ccgatggtca gtgttggaga cagcgttcga cgcggtcaga agatcgcccg
      361 gatacctgaa ggcaagttgg gagccaatat ccatgcgagc attgatggcc gtgtgacaga
      421 gatcgccaac ggtgttgtca ggatacagca gtgaactgcc agcccgtcgt tcttacggtg
      481 gatgtcgaag actacttcat gagtccggaa tccatcgcat tcgagcagtg gagctcgttc
      541 gaagatcgtg tccaggtcgg gctcaatcgc cttcttgaca tgctcagcac ttgtgggcga
      601 accgcgacgt tcttctttct tggctatgtt gcggaacgac atcccgaagc agtaatatct
      661 gtcgcaaagg caggccatga ggtcgctgtc catcattact ggcatcagcc gtatgagcag
      721 atcggaaagg cacgctttct cgaaggactc agacggacaa agggtattct tgaagatctc
      781 atcgggacga aagtcatcgg acatcgagcc ccgatgtgga gtgtacgaag ggatatgccg
      841 tgggtctttg aaggactcct ggagaatggc ttccaatacg attcgtcctt gttcccaatt
      901 cggacttatc tctacgggga tgctggcgcg gagccggagc cacattggct gaactttggc
      961 ggtgggcgca ttctggaggt gccaccgtcc acgcagacag tcttcggacg ccggattcct
     1021 gtcggcggag gcttcttcct gagagcctat cctctaggga tgacgcgatg gggaatcaat
     1081 cgccggttgc gttccgggcg ttcggtagta ttatacgttc atccgtggga attggatccg
     1141 ggacatcccc ggctccaact gcccacgaag ttggcgtgga ttcatgccgt cggcttgaac
     1201 cgtcttggac gcaagatgac agagctggtg aaacaatatg agtttacatc gcttggcaac
     1261 gctctgagcc gattcgaagc gattgcggga ggcacaaatg cctgaggctg ttggcgtagt
     1321 cgaatggaga agcattgcca tcgctatcga agcgacggac gcaatgcttg acgcggcaga
     1381 ggttgaggtc ttggtttcaa cacccgtctg ctcggggaaa tatgtcacga tcgtgagcgg
     1441 cgatgttgca tcggttcagg ttgccgtgga aaccggagtt cgtgaaggtg ggaattctct
     1501 ggtcgaagca gtcgtcattc cgaacgtcca tccggatatt caaccggcga tcactgccac
     1561 aaccggtgtc gagaacgtgc gaagcctggg agtgattgaa gtcttctccg tcgccggtgc
     1621 agtctatgcg gcggatgcat cggtcaagtc tgcagatgtg agactgattg aaattcgatg
     1681 cgctcgcggt ttgggtggaa aggcgtttgt gaccctgacg ggagatgtgg acgctgttcg
     1741 aaccgcaaca gagaccggtg ccgccattgc tcgtgagaaa ggacttctcg cggcgagcgt
     1801 tgtgattccg gacttgaatg agaaactgcg ccgatttgtt ctttagagtg agcccgttgg
     1861 tcaagggagc ggtctgaagt tgtcattctg aacgaagaga agaatcgctc ttcctgtcgc
     1921 agaatgcact attgtgaaag cccttcgcag tttctggagt ttgcaggatg gatagtgcac
     1981 caccagttgc cctcagtcta gtcattcccg tctacaatga gaaagatgcc gttgcggaaa
     2041 ctctcgttcg cgcgtgcagc gtaatcgaac aatcggggat ttcggcggag atcatcgttg
     2101 tggatgacgg ctccgatgac ggaaccggtg acgttctgaa gaactttggt tcctcagtgc
     2161 agattgtccg acatccggtc aatcgcggct acggagcggc gatcaagacc ggtctgcgac
     2221 atgcccaagg cgagtggatc gccatcacag atgctgatgg aacctatccg ctggagaagc
     2281 tgccggagtt gtggaatctg acgcaggatg ccgagatggt cgttggcatg cgacctagaa
     2341 gtgcgcaacc gatgattcga cgaccggcga aatggttcct ggctcggctc
//
LOCUS       DAOQUC010000584         2387 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_54626_length_2387_cov_3.160377, whole genome shotgun sequence.
ACCESSION   DAOQUC010000584 DAOQUC010000000
VERSION     DAOQUC010000584.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2387
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54626_length_2387_cov_3.160377"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1966
                     /locus_tag="PKH07_10360"
     CDS             <1..1966
                     /locus_tag="PKH07_10360"
                     /inference="COORDINATES: protein motif:HMM:NF019311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PA14 domain-containing protein"
                     /protein_id="HNT35386.1"
                     /translation="PPLSVLFVVLCFFFLLRGLRGRRCTDFLLCGAMAGFGIMTYAPT
                     RLLVVMLVVLFLHQCLSRKKFFRTFWYAPLLIMLGGQLTSCPINVAAFTDSAMFTARM
                     KQTTVFKAGKPREQAWKDVRMSTGKHLLMFNYKGDPNARHGIALSPKLDPLTAAFFAL
                     GILVCVFRWRHWESALLAFWLLIGLLAGILTLEWEAPQSCRTQLVIPAAMLLAGVPFS
                     IFWHQLKGLWGRWRWIPFFAAVLPLLVWLTIWNYRYYFVRTQADQNVYEEFVGRDTSI
                     ARYLATQDLDENFFVHQQSPNDYPAIPFLLNRRDIPGVLYQMPDYIPMRMQTGKKLHY
                     LLEPYRTPFPEELFKHYYPGGQYHLFEDPWKKPIFYTYVVSADEVASIIGLSASYRAI
                     SDPLGQQGEWKRVERSPFDLLVGDNAPSTFPVRGHWTGSVFIEQSADYLLSWSDGEPI
                     CIALDNEPLPEEGQPEIPVSLIRGWHNVEIVRTVNSANSPFRLMFKMGAAKAEQFKSE
                     AFCARTMPDLGYYGRYYANLDWAQPASYGIIQPTISIRWHPEPVRGRLHWSGKWSAYL
                     NAEKPGQYGFLIRCNAYCRIEVDGKQVLEQPERHQNPGRANIPLEAGEHVLEIFYKEP
                     GQYSELHVLWAPPGGREEIIPYDKIRPKFD"
BASE COUNT          535 a          611 c          641 g          600 t
ORIGIN      
        1 gccgccactc tcggttttgt ttgttgtcct ctgcttcttt ttcctgcttc gagggcttcg
       61 cgggcgtcgt tgtaccgact ttctcctctg tggagcgatg gccggttttg gcatcatgac
      121 ttacgcgccg acaaggctcc ttgtcgtcat gctcgtcgtt ctctttctcc atcagtgtct
      181 cagtcggaaa aagttcttca gaacattctg gtatgctcct cttctgatca tgcttggcgg
      241 acagctcacc agctgtccaa tcaacgtagc agccttcacg gattcggcga tgttcaccgc
      301 ccggatgaaa cagaccacgg tgttcaaagc gggcaaacct cgcgaacaag cctggaaaga
      361 cgttcgcatg agcactggca agcacttgct gatgttcaac tacaagggcg atccgaacgc
      421 gcgtcatgga attgcacttt ctccaaaact ggatccattg acagccgcct tcttcgcact
      481 gggtattctt gtgtgcgttt ttcggtggcg gcactgggag tcggcgctgc ttgcgttctg
      541 gctcctgatc gggcttctgg ccgggattct gacactggaa tgggaagcgc cgcagtcctg
      601 tcgaacacag ttggtgatcc ccgccgcgat gctgcttgcc ggtgtgccgt tctccatctt
      661 ctggcaccag ttgaaggggc tttggggacg gtggcgatgg attccgtttt ttgccgctgt
      721 gctgccgctg ctggtctggt tgactatatg gaactaccgt tattacttcg tccgaacgca
      781 ggcggatcaa aacgtctatg aagaattcgt ggggcgcgac acaagcattg cgcgatatct
      841 ggcgacccag gatcttgacg agaatttctt cgtgcatcaa cagtcgccaa atgactatcc
      901 ggcaattccg ttccttctca acaggcgaga catccctgga gtcctctatc agatgccgga
      961 ctatatcccg atgcgaatgc agaccggcaa gaaactgcat tatctgttgg agccctatcg
     1021 aacgccattc cccgaagagc tcttcaaaca ctactatccg ggggggcagt accatctgtt
     1081 cgaagacccc tggaagaaac ccatattcta tacctatgtc gtatcggcgg atgaagtggc
     1141 ttcaatcatt ggtctctctg cgtcctatcg cgcaatatcc gatccgttgg gtcagcaggg
     1201 agaatggaaa cgtgttgagc gctctccttt tgatctgctg gtcggtgaca acgcgccaag
     1261 cacatttcct gttcgagggc attggactgg aagcgtcttc attgagcagt ctgctgatta
     1321 cctgctctcc tggagtgatg gagaaccgat ctgcattgcc ttggacaatg aaccgttgcc
     1381 cgaggaaggc caaccagaaa tacctgtgag tctgattcga ggctggcaca acgtggagat
     1441 cgtgcgcacc gtcaacagcg caaacagtcc attccgcctc atgttcaaaa tgggggcggc
     1501 taaggcagaa cagttcaaat ccgaggcatt ctgtgcgaga accatgccgg atttaggcta
     1561 ctacggacgt tattatgcca acttggactg ggctcaacct gcgagttatg ggatcatcca
     1621 gccgacaatt tccattcgat ggcaccccga acctgttcga ggcaggctgc attggtcggg
     1681 gaaatggtct gcctatttga acgcagaaaa acccgggcaa tacggttttc tgatccgatg
     1741 caacgcatat tgccgaatag aggttgacgg aaagcaggtg cttgaacagc ccgagagaca
     1801 tcaaaatcca ggacgcgcca acattcctct ggaagccggc gagcatgtct tggaaatctt
     1861 ctacaaggaa ccgggacagt actcagaatt gcatgtgctc tgggcaccac cgggaggtcg
     1921 tgaggagata atcccgtacg acaagattcg cccaaagttt gactgatcct ctctcctgaa
     1981 accagattca agaaatcatg attctcttca gaagaatgcc tggaggggcg ggtaaggttt
     2041 gggctcctgc agcagcttgc aagggatcag gttcggacgg tataggggaa atcttccgga
     2101 cagaatccgg acaaattcga ttgacaactt tttgagaatt cagtatatat ggaatctgta
     2161 aggtagcgag gagatctgtg aacttagccg catctggaca tttctcacag actgattctt
     2221 aacgttctag taaacgatta catcatctag gttctggttc tcctacgagc gaacatcttc
     2281 atcgcttgct tgttgagcct ctgtagctat cagtgatagg ggctccttgg tggcggatag
     2341 aggaggtggt tgtagggcat aacaacagag aagaggcttc ttcggtc
//
LOCUS       DAOQUC010000585         2380 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_54901_length_2380_cov_3.304086, whole genome shotgun sequence.
ACCESSION   DAOQUC010000585 DAOQUC010000000
VERSION     DAOQUC010000585.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2380
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54901_length_2380_cov_3.304086"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2344)
                     /locus_tag="PKH07_10365"
     CDS             complement(<1..2344)
                     /locus_tag="PKH07_10365"
                     /inference="COORDINATES: protein motif:HMM:NF021778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HNT35387.1"
                     /translation="MGVAVTPDGSYVLVSNRTTGADGLPDTVMFINTKTNAVAKTLTV
                     EDGPSSVAITPNGQYAFVTNEFSATVSVIDVPNQVVVDVNPGTPEIDAISLGNPAAAP
                     VDIISTLDSSKIYVANFAISTISVIDVATLQVVNSYALGTSPNGIAITPNGTKLYVTN
                     FNAENVLVLDAATGTALPSSPINIGIIAGPPKVNERPQSIRITPDGTKAYITIDGKQG
                     NQPDFVRCIDTVTDTLIPGVNISLGVNSTPRGIAITPMGDYVYVCKSAGTDVVVISSA
                     SNTIAHTLTTALNPRQVAIVGSVFLPPPEVTLVSPGGGPSSGGTPVTVTGKYFREGAT
                     VTFGSTPATAVQVQSLTQLTCIAPAGPANQPVNVTVANLGDGSGVLTNGYFYDPPPVL
                     YSVLPNEGPEAGNTSVTISGRFFDSGLTVAFGSVAATDIVVVNSQTVTCKTPAGVGIV
                     NVTLRNPDLQQATLTNAFTYYPPPDILSITPDSGPEAGGTVVGISGTGFRAGVQVFFG
                     ATQSPSVVFSSSTSLDAVAPEGYGVGTVAITVRNSDGQQDILDAAFRYVPAPEILSIA
                     PGQGPENGGTLVSIFGDHFSSPGAAVRIGGKNALSTTVVSATEITFLTPSGVGSVAIR
                     VTNLDGQYDEVSDAFLYVPAPVVLSVDPTSGNQTGGTPVTINGLHFHPDATVSFGPYG
                     AASVMVHDHAVIWATTNPGVGVVNVRVTNPDGQYGSLPGGFTYITPVIVSQVLPVKGP
                     TAGNTVVEIAGAGFVAGATVEFGGLLAGSVVVDSSAHLTCL"
BASE COUNT          599 a          637 c          637 g          507 t
ORIGIN      
        1 tcaaacatgt cagatgtgcc gacgagtcca cgacgacgct tccagcgaga agtcctccaa
       61 actcgaccgt cgcaccagcc acaaatccag caccagcaat ctcaaccacg gtatttccag
      121 cagtcggtcc tttcaccgga aggacttggc tgacgatgac cggtgtgatg taagtaaacc
      181 ctccgggcaa cgaaccatat tggccatccg gattggtgac tcgaacgttg accaccccaa
      241 ccccagggtt ggtggtcgcc cagatcacgg catggtcatg taccatgaca gacgcggcac
      301 cgtagggtcc aaaagaaacc gttgcgtctg gatgaaaatg caatccattg atcgtaacag
      361 gggtaccccc tgtctggttg ccgctggtcg ggtcaacgct gagtacaaca ggcgctggaa
      421 catagaggaa agcgtcagac acctcatcgt actgaccatc gagatttgtg acacgaatcg
      481 ctacagaccc aacgcccgac ggagtcagaa atgtgatttc ggttgccgag acaacggtag
      541 tactgagtgc gtttttgcct ccgatcctga ctgctgcccc gggactgctg aaatggtcac
      601 caaaaatgct gacaagtgtt ccgccattct ccggaccctg gcccggagca atgctaagaa
      661 tctccggcgc aggaacataa cggaaggctg catccaagat gtcctgctga ccatcagagt
      721 ttcgaactgt aatcgccact gttccaactc catagccctc gggtgcaaca gcgtctagac
      781 ttgtcgaaga agagaacaca acggaaggtg actgtgtggc tccgaagaaa acctgcactc
      841 cagccctgaa tccagttcct gatatcccca caacagtccc tcccgcttca ggtccagaat
      901 cgggggtgat tgagagaatg tcgggaggtg ggtagtatgt aaaagcattg gtcagtgttg
      961 cctgctgaag atcgggattc cgcaaggtca cgttcacaat tcctacaccg gcaggcgttt
     1021 tgcatgtgac cgtctgggaa ttgaccacaa caatatccgt tgcagcgaca gaaccaaaag
     1081 ccaccgtaag tccgctgtca aagaatcgtc cagagatggt aacggaagtg ttcccagcct
     1141 caggcccttc atttggcagg actgaataga gaactggagg aggatcgtag aaatacccgt
     1201 tggtcaacac tccactcccg tcgccaaggt tggccacagt gacattgacg ggttgattgg
     1261 caggtccagc gggagcgata caggtaagtt gtgtcagact ttgcacttgc acagcagttg
     1321 ccggagtgct tccgaatgtc accgtcgctc cctcacggaa gtactttcct gtaacggtca
     1381 caggtgtacc accactggac ggaccacccc ccggactgac gagagtgacc tcaggaggag
     1441 gaagaaatac agagccgaca atggcaacct gcctcggatt caaggcagtg gtgagcgtgt
     1501 gagcgattgt attgctcgct gaggagatga ctaccacatc tgtgcccgca gacttgcaga
     1561 catagacata atctcccatc ggtgtgatgg caatacctct gggagtagag ttcacgccaa
     1621 ggctgatgtt tactccaggg ataagcgtgt ctgtgaccgt gtcaatgcag cgcacaaagt
     1681 caggctggtt gccctgcttt ccgtcaatcg taatatatgc cttggttcca tcaggtgtga
     1741 tacggatgga ctgtggacgc tcgttcactt tgggcggacc tgcaataatg ccgatattga
     1801 ttggggaaga aggaagagcg gttccagttg ccgcatcaag aacaagaaca ttctctgcgt
     1861 tgaaattcgt cacgtacagt ttcgttccat tcggtgtgat cgcgatacca ttgggacttg
     1921 tcccgagagc gtaactgttc actacctgaa gtgtcgccac gtcaatgaca gatatcgtgc
     1981 tgatggcaaa gttagccacg tagattttcg aactgtccag cgtactgatg atgtcaaccg
     2041 gagcagcggc agggttcccc agagaaatcg cgtcgatctc tggagtgccc gggttgacat
     2101 ccacgacaac ctgattggga acgtcaatca ctgagactgt cgccgagaac tcgttcgtaa
     2161 caaacgcata ctggccattg ggagtgatgg caacagaact aggaccatcc tcgacagtca
     2221 gagtcttggc cacagcgttt gtcttggtgt ttatgaacat cacggtgtcc ggaaggccat
     2281 ccgcaccggt agtgcggttg gagaccaaga cgtaactgcc atcaggagtg accgcaacac
     2341 ccacagggcc atctccaact gagatgccta ctccaacaac
//
LOCUS       DAOQUC010000586         2379 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_54941_length_2379_cov_3.358864, whole genome shotgun sequence.
ACCESSION   DAOQUC010000586 DAOQUC010000000
VERSION     DAOQUC010000586.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2379)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2379)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2379
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54941_length_2379_cov_3.358864"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1373)
                     /locus_tag="PKH07_10370"
     CDS             complement(<1..1373)
                     /locus_tag="PKH07_10370"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HNT35388.1"
                     /translation="MSSSKAQSLGPNVRVGVLQKVPRVRFMANQILDVFAADEKTRLG
                     EIPAKTECIAVLADAVAARIAYRVRLGVFDHPDDATQILDDPALASCSGKVKAAGRPM
                     LVAGETISDNREYWALTGEFATEHDAQTLWDTLSPKYPEALVMKDVLVPPKGRVQILE
                     SQSGKVLFESDRKIRVSPRDFTKTRIAIYDVIVGIEFHWEHKETQAFRGLYDFVVDNS
                     GALTAVNELSLEEYLYSVNSSEMLSICPEELLKAQTVCARNTILATVARHHVDDAFDI
                     CADDHCQCYRGSTREAPSSVKTANETRGEVLVFEGKVCDTRYSKICGGIMEPAVNVWW
                     EGHIPYLDAGKDAAAQQAESISYVTNEQQAKAWIDSTPSVFCNTQVENLPEELHYARK
                     YFRWRVEYQRTELENLIKERANVDVGTVLDLVPVERGQSGRIMWLDIVGTSGTKRIGK
                     ELAIRRAL"
     gene            complement(1363..>2379)
                     /locus_tag="PKH07_10375"
     CDS             complement(1363..>2379)
                     /locus_tag="PKH07_10375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35389.1"
                     /translation="GRVVPPDSGVPNGLEPFLNENGVLIQPIIRRRHYLTKDICHMNV
                     ARAKRPGNVSGRGVSSPAVAKDDPERCPFGPGKEAMTPRWEGTGSDYVRIGTDPWQVR
                     SFPNLYPWMLDHLNIVETPLHKASSQEVDPNEEALALQVAAQYVQEQELLGRCVVLFR
                     NQGLSASLAHFHWQIGSLSYVPVREAEELSRAEQFHRDHGMNIFDAILDAERRRGERL
                     VGENAAFSVLAPFAPRTNNEIWIILRQAASSLSQISETDRRNLAEILCVSIKGLFAIS
                     GNDDLFVIVHQLPPSYRAYRFHVELFPVKPWSGAERGFGELVVEKAPEDTATELREKM
                     KDVF"
BASE COUNT          539 a          693 c          606 g          541 t
ORIGIN      
        1 agtgctcgtc gaatcgccag ttccttgccg attcgcttgg tgccgctggt tccgacgatg
       61 tccaaccaca tgatgcggcc ggactggcct cgctccactg gcaccaagtc cagcaccgtt
      121 cccacgtcaa catttgcgcg ttccttgatc aggttctcca attcagtgcg ctgatactcg
      181 actctccaac ggaagtactt tctggcgtaa tgaagttcct cgggcagatt ctcaacctgc
      241 gtgttgcaga acacactcgg agtcgagtca atccaggcct ttgcctgttg ttcgtttgtg
      301 acgtagctga tgctctctgc ctgttgggca gcagcatctt ttcctgcatc cagataggga
      361 atatgccctt cccaccatac attgacggcg ggttccatga tgccaccaca aatcttcgag
      421 taccgcgtat cacacacttt gccttcaaag accagcactt ctccccgagt ttcattggcc
      481 gtcttgaccg aagacggtgc ttcgcgggtg gaaccccgat agcactgaca gtggtcatcc
      541 gcgcagatgt caaacgcatc gtccacatgg tgtctggcga cagttgccaa aatcgtgttg
      601 cgcgcacaga cggtctgagc cttcagaagc tcttccggac agatggagag catctcagat
      661 gagttgacgc tgtagagata ctcctccagt gacagttcat tgaccgccgt caacgctccg
      721 gagttgtcca caacaaaatc atacagaccc cgaaatgcct gcgtttcctt atgttcccag
      781 tggaattcga tgccgacgat aacatcgtag atggcgatcc gggtctttgt gaaatcacgt
      841 ggagaaaccc gaatcttacg atccgattca aagagcacct tgcccgattg cgactcgaga
      901 atctgaacgc gtcccttggg gggcacaagc acgtctttca tcaccagcgc ttcgggatac
      961 tttggactca gagtgtccca caaggtctgc gcatcgtgtt ccgtcgcgaa ctctcctgtc
     1021 aatgcccagt attcgcgatt gtcgctgatg gtctctcccg caaccagcat cggtctgccg
     1081 gcagccttca ctttgcctga acaactggcc aaggcggggt cgtccagaat ctgagtcgcg
     1141 tcgtcgggat ggtcgaacac tccgagtcga acccgatagg cgatgcgcgc ggcgacagcg
     1201 tcagcaagca ctgcaatgca ctctgttttc gcggggattt caccaagtcg agtcttctca
     1261 tcggcggcga aaacatccag aatctggttt gccatgaacc gaacacgagg cactttctga
     1321 agcactccga cgcgcacatt ggggccaaga gattgagcct tgctagaaga catctttcat
     1381 tttctcccgt aattctgttg ccgtatcctc aggagctttc tccaccacca actccccaaa
     1441 acctctttca gcacccgacc agggtttgac gggaaacagc tcgacatgga atcggtatgc
     1501 ccggtacgag ggtggaagtt ggtgaacgat gacaaacaaa tcatcatttc cggaaatggc
     1561 aaaaagccct ttgatgctca cacagagtat ctcggcaaga ttccttcgat ccgtctcgga
     1621 aatctggctc aaacttgagg ctgcctgccg caggataatc cagatctcat tgttggttcg
     1681 aggtgcaaag ggggcaagaa ctgaaaaagc agcgttctct ccaacaagac gttcccctcg
     1741 gcggcgttct gcatccagga tcgcgtcaaa gatgttcatc ccgtgatctc ggtggaactg
     1801 ctcggcacga ctgagttcct ccgcctctcg aacgggaacg taagaaagcg acccaatctg
     1861 ccaatgaaaa tgagccagtg aagcggaaag cccctgattg cggaacaaca caacgcaccg
     1921 gccaagaagt tcctgctcct ggacatactg cgctgccacc tgcaaagcca aggcctcttc
     1981 gttcgggtct acctcttgcg aactggcctt gtgcaaaggt gtctcgacaa tgttcagatg
     2041 atccaacatc caagggtata ggttgggaaa tgaccgcacc tgccaaggat ccgtcccgat
     2101 gcggacatag tcggatccgg ttccctccca tcggggcgtc atggcttcct taccaggacc
     2161 aaagggacat cgttccggat cgtctttggc cacggccgga gaggagacac ctctacccga
     2221 aacgtttccc ggtctcttgg ctcgggcaac gttcatgtga cagatgtcct tggttagata
     2281 gtgacgccgt cggatgatgg gctgaatcag gacaccgttc tcattcaaga aaggctcaag
     2341 gccattcggt acgccggaat cgggcggcac aactcgacc
//
LOCUS       DAOQUC010000587         2377 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_54997_length_2377_cov_3.844100, whole genome shotgun sequence.
ACCESSION   DAOQUC010000587 DAOQUC010000000
VERSION     DAOQUC010000587.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2377
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54997_length_2377_cov_3.844100"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(114..1460)
                     /locus_tag="PKH07_10380"
     CDS             complement(114..1460)
                     /locus_tag="PKH07_10380"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007279862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase class II"
                     /protein_id="HNT35390.1"
                     /translation="MPKQQPWTPDSWKSRPVEQQPEYPCPQECDRVLERIQSYPPLVV
                     VGEVEKLKEQIALAGAGKAFILQGGNCAERFIDCNETNVANKLKILLQMSVILTYGVR
                     RPVIRIGRIAGQYAKPRSKPTEIVNGKEMPSYRGDAVNSMEPVLDTRIPDPSRLESSY
                     FHAAATLNHIRALLDGGFADLHDPYRWNLDTIESSEKWHEYSHVVERILDAIGFMESF
                     GGVREEVLGRVDFYTSHEGLLLGYEQALTRPDVTAKRYYNLGAHFVWIGVRTRQISGA
                     HVEYFRGISNPIGVKVDSTCTPEELVSLIGILNPGNEPGRLTLITRFGARKIDQFLPP
                     LLHAVRKSKRQVTWVCDPMHGNTTQARNGTKTRDFSLIVQELHQTFQIHKELDSCLAG
                     VHFELTAENVTECVGGAGDLSSEDLSRRYETYCDPRLNDSQSLEMAFLIARWLQTP"
     gene            complement(1511..2365)
                     /gene="panB"
                     /locus_tag="PKH07_10385"
                     /pseudo
     CDS             complement(1511..2365)
                     /gene="panB"
                     /locus_tag="PKH07_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004093247.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
BASE COUNT          528 a          675 c          621 g          553 t
ORIGIN      
        1 tcatcttctc gatgaaggtg gctaccgcct tgacttcgtg ggtatgttcg aactcgtcac
       61 caatccagac cttgactgcc atcacgcgcc tcctgatgag aagtcatttt tttctacgga
      121 gtctggagcc agcgtgcgat gagaaatgcc atctccaggc tttgagaatc attcagtcgt
      181 gggtcgcagt aagtttcata gcgtcgggag aggtcttcgc tgctcaaatc accggcaccg
      241 cccacacatt cggtgacgtt ctcagcggtc agttcaaagt gaactccggc gagacaggaa
      301 tcgagttcct tgtgaatctg gaaagtttga tgcaactcct gaacgatgag agaaaagtca
      361 cgggtcttcg ttccattccg cgcctgtgtg gtattgccgt gcatgggatc gcagacccag
      421 gttacttgtc gtttggattt ccgaacagca tgcagaagag gaggcagaaa ctggtcaatt
      481 tttctggcac caaaacgggt gataagcgtc agacgccccg gttcatttcc gggattcaga
      541 atgccgatga gtgagaccaa ttcctcaggt gtgcaggttg aatccacctt cacgccaatg
      601 ggattgctga ttccgcgaaa atactccaca tgcgctccag aaatctgccg cgttctcacg
      661 ccaatccaga cgaaatgtgc accgagattg taatagcgct tggcggtgac gtcaggtcga
      721 gtcaacgcct gctcgtaacc cagaaggagt ccttcgtgag aggtgtagaa atccacgcgt
      781 ccgagaactt cctctcgaac gccaccgaac gattccataa atccgatggc atccaaaatc
      841 cgttctacaa cgtgactgta ctcatgccac ttttcgctgg attcgatcgt gtccagattc
      901 catcggtatg gatcatgaag gtcggcgaat ccgccatcca agagtgctcg aatgtgattg
      961 agtgtcgccg ccgcatgaaa gtaactggac tcaagacggc tcggatcggg aatgcgagta
     1021 tcgagcacgg gctccatcga attgacggca tccccacggt aacttggcat ctccttgccg
     1081 ttcacgatct ccgtcggttt ggagcgaggc ttggcatact gaccggcgat gcgaccgatt
     1141 cgaatgaccg gacgacgcac accataggtc aagatcacgc tcatctgaag caggattttg
     1201 agcttgttgg cgacgttggt ctcgttgcag tcaataaacc gttctgcgca gttgcccccc
     1261 tgaaggatga acgccttgcc cgctcccgcc agggcgatct gctctttgag tttctccacc
     1321 tcaccgacga caaccaaagg tgggtaggac tgaatgcgtt ccagtacccg atcgcactcc
     1381 tgcggacagg gatattccgg ctgttgctcc acgggacggg atttccagct gtccggcgtc
     1441 cagggttgct gcttcggcat tcctgattcc tttctttcaa tgacgcgccc aaatgccaaa
     1501 gcgcggctct ttacgagaat atctcgtcca gtgatttcaa cagagactcg tctccaagag
     1561 cgtagctgtg ttcctcatcg gggaaatcac cgttgcgaac atctttgaca aaggagtcta
     1621 tcccgcgaag agcggttccc gccaattccg cgtaccgttt gacaaatttc ggtttgaact
     1681 ccgtgaaaaa gccaagcaaa tcctgagtga ccagaacctg accggaacag cctgatccag
     1741 cgccgatccc gatagtcgga attcggagtc ttgccgtcag ggctttggcc acggctgcag
     1801 gaaccgattc gatcaccagg gcgaacaccc cggcatcctg aaccgccttg gcatcttccc
     1861 aaagtttctc cacggccacg cgatccttgc cgatgaccgg aaaaccgcca tagtgcatga
     1921 cctgctgcgg ctgcatcccg atgtgtccca tgacggggat tcccgtctcc acgacagcat
     1981 ggattcacgc tgccatggcg agaccgccct ccagtttgac cgcgtgtgcc cctgcctgct
     2041 tgagcatcac accgcagttg cgtatggcgt cttcggaact ggtctgatag gacaggaaag
     2101 gcatgtctga gaccaccatc gcatgttgag cgccgcgtga tacgacacgt gtgtgatagg
     2161 ccatttcttc aacagtcacc gacagcgtgt tcgattcccc tttgaatgtc atgccgaggg
     2221 agtcgcccac caggatgata tccacaccgc attgatcgag ccaactggcg gtcagatagt
     2281 cgaaggccgt aagcatcaca atcggctcgc ccacttcata ctgcttgcga accgtaacgg
     2341 tggttttctt gctcagttga ttcatggtcg tctcgct
//
LOCUS       DAOQUC010000588         2374 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_55130_length_2374_cov_3.007331, whole genome shotgun sequence.
ACCESSION   DAOQUC010000588 DAOQUC010000000
VERSION     DAOQUC010000588.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2374
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55130_length_2374_cov_3.007331"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..432
                     /locus_tag="PKH07_10390"
     CDS             <1..432
                     /locus_tag="PKH07_10390"
                     /inference="COORDINATES: protein motif:HMM:NF012443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP--guanido phosphotransferase"
                     /protein_id="HNT35391.1"
                     /translation="EGSENTGNLFQISNQITLGATEEELIQKIANIAEQIAKDERRVQ
                     RELRKHAARLIEDRVWRAYGLLTNARLLTSREVLENLSDIRLGISLGLVEHVDFRTCN
                     EILFYAQPAHLQRMAGQPMEASDRDAFRADYVRRNLTKENP"
     gene            742..>2374
                     /locus_tag="PKH07_10395"
     CDS             742..>2374
                     /locus_tag="PKH07_10395"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HNT35392.1"
                     /translation="MLGLISESEGVAAIALQNMGVDLEALRAEIFRRTRQEPSGFPKG
                     EPQPSLSARRAIEGAMKEAQSMQVNWIGTEHLLLGVIREQEGLGAEILTDMGVDLDRA
                     RAEVYRLLGGSPPPETSHQAKQKSKTPALDAFGNDLTEMARQGKLDAVIGREDEIERV
                     IQILCRRTKNNPVLIGEAGVGKTAIAEGLAQKIVNKTVPDLLANRRIVTLDLGALVAG
                     TKYRGQFEERLKAVLDEIRRMENVTLFVDELHTLVGAGAAEGSMDASNMLKPALARGD
                     VQCIGATTMEEYRKYIEKDSALERRFQTILVNPPNQEQAYEILLGLRERYEAHHHIKY
                     TNHALKAAVDLSDRFITERHLPDKAIDLMDEAGSRARLQATTKPQAIRDMEKRIEEIN
                     KEIDTATLTENFERCAELKRQRDELDAQLKAQISQWEKDKSDQQTPVVNEEEIAYLVS
                     KWTGIPVFKIEEKESEKLLRMEDELHARIIGQKDAIRIVSTCIRRNRAGLSDPKRPIG
                     SFLFLGPTGVGKTELARALAEFLFGDEDAVVTLDMS"
BASE COUNT          609 a          616 c          666 g          483 t
ORIGIN      
        1 gaaggcagtg agaacacagg aaatctcttt cagatctcca accagattac actcggcgct
       61 acggaagaag agttgatcca gaagatcgcc aatattgctg aacaaatcgc gaaagacgaa
      121 agacgtgtcc agagagagct tcgaaaacac gccgctcgac tgatcgaaga tcgggtctgg
      181 cgtgcctacg gtctgctcac caatgcccgg ctgttgacat cccgcgaggt cttggaaaac
      241 ctctccgaca ttcgcctggg gatttcgttg ggtctggtgg agcatgtgga ctttcgcacc
      301 tgcaacgaaa tcctcttcta cgctcagcct gcacacctgc agagaatggc tggccagccg
      361 atggaagcgt cagacagaga tgcctttcgc gctgactatg tgcgcagaaa tctgacaaag
      421 gaaaacccct gagcacaata aagtttgcgt tttatgtgcg tctacactac attgtgcagc
      481 ctgttgaagt attcgccaca atatgttgtg ggcatgtgag acaacacgct ctctttgtca
      541 aaagggtatg gattgtctga gagcttttga gtattattac tctgtttttc tcaacggatt
      601 agggaacttc tgagcgtcgc cagacgcacg aaagaaaaat atatgtacga gcactatacg
      661 gaacgggcac ggcaagttat gcggcgcgcc aaggacgaag ccgcaaaagt gggctccaca
      721 cagttttcca cggagcacat cttgcttggc cttatttctg aaagtgaagg cgttgcagcg
      781 atcgccctgc agaatatggg tgtagacttg gaggcgcttc gtgccgagat tttccgacgc
      841 acccgacagg aacccagtgg ctttcccaaa ggggagcctc aacccagtct gagcgcacga
      901 cgtgcaattg aaggcgcgat gaaggaagcg cagtcaatgc aggtgaactg gattggaaca
      961 gagcatctgc tcttgggtgt tattcgcgag caggaagggc ttggagctga gatccttacc
     1021 gatatgggag tggatcttga ccgagcacgc gcagaggtct atcgccttct ggggggctcg
     1081 ccgccaccag agacctcgca tcaggcgaaa cagaagagta agacacccgc attggatgcc
     1141 ttcggcaacg atctcactga gatggcacgt cagggaaaac tggacgccgt catcggtcgc
     1201 gaggatgaga ttgaacgtgt catccagatt ctgtgccgac gtaccaagaa caatcctgtt
     1261 ctcattggcg aggcaggcgt aggaaaaaca gccatcgccg aggggttggc gcagaagatt
     1321 gtcaacaaga cagttcccga ccttctcgca aatcgccgaa tcgtcacctt ggacttgggt
     1381 gcattggttg ccggtaccaa gtaccgaggc cagttcgaag agcgtctgaa ggccgttctg
     1441 gacgagattc gacgcatgga gaatgtcacg ctctttgttg atgagttgca caccttggtt
     1501 ggagcgggtg ccgcggaagg ttccatggat gcatcgaaca tgctcaaacc ggctcttgcc
     1561 agaggcgatg tgcagtgcat cggcgcaacc accatggaag agtaccgcaa gtacattgaa
     1621 aaggacagcg ccctcgaacg ccgttttcaa acgatcctcg tcaacccgcc caatcaggaa
     1681 caggcctatg aaatccttct cggtctgaga gaacgctatg aagcgcatca tcacatcaag
     1741 tacaccaacc atgccctcaa ggcagccgtg gatctctctg accgatttat taccgaacga
     1801 catctccccg ataaggccat tgacctgatg gacgaagccg gctctcgggc tcggcttcag
     1861 gcaaccacca agccacaggc cattcgggac atggaaaagc gcatcgagga gatcaacaag
     1921 gagattgaca ccgcgacact gacagagaat ttcgagcggt gcgcggaact caagcgacag
     1981 cgggacgaac tggacgcgca gctgaaagcg caaatctcgc agtgggaaaa ggacaaatcc
     2041 gaccagcaga ctcccgtcgt caatgaagaa gagatcgcct accttgtctc caagtggacg
     2101 ggaatccccg ttttcaagat cgaggagaag gaatcagaga aactgctgcg aatggaagac
     2161 gaactgcacg ctcggatcat tggccagaaa gacgcaatcc gaatcgtgag cacatgcatt
     2221 cgtcgcaatc gcgccggcct gagtgatccc aagcgtccca ttggttcctt tttgttcctg
     2281 gggccaaccg gagttggaaa gaccgaactt gcgcgggcgt tggccgagtt tcttttcggc
     2341 gatgaggacg ctgttgtcac gctggacatg tccg
//
LOCUS       DAOQUC010000589         2367 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_55406_length_2367_cov_3.377595, whole genome shotgun sequence.
ACCESSION   DAOQUC010000589 DAOQUC010000000
VERSION     DAOQUC010000589.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2367
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55406_length_2367_cov_3.377595"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2367
                     /locus_tag="PKH07_10400"
     CDS             <1..>2367
                     /locus_tag="PKH07_10400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35393.1"
                     /translation="VDGETLLVFLGIDEQVEALDAPRVQAFHLEFQHPQYTGLPDRSE
                     RKLDGNILAPKGTLVRMRVQSNNPLESAGIAFVGGRKCPMEILAESVASATFTVQDST
                     RYQVFLKDTFGLALTDGPFYQVVAKADSSPSVSFVAPAVQVMDVPEEMTVPVSGQAED
                     DYGLTKAEFIYQVGYDQPERVTEMPLKPLAMDAQLDSATPRTAEIAFEWDLRTLDLLP
                     GESVSYWLRVFDNDPVDGPKEGMSGVQVLRFPSLFEIYEEVEKQELAQVDSMDDLLQK
                     QREIQKETDALRETIQRDAEKPEEQVPWEQKETLKDLKTRQEQIQKELEQLQNLFLSG
                     QIQKEMEQLQQQYDQSVTQLKEDTAISSQTFEKMAKIQQLLDQMMTSEMKETLQKFNQ
                     ALEQITAKQMDKSMGDLNSSMQQFEESLDRTLSLLQQNYMERSLERLSYQAEQMAKTQ
                     QEIQDRTQQRSAEDPLDDLARRQQRLQKTAEEFIKDVSALAQMAEQTDNKEMKEKLDE
                     VLKQAEQKELQQSLEQAQESLSEGKKDQALQQQQKAQEALDQMSAMMNECKECMSGGM
                     SVTFDVQAWHRLMDRVFHVSESQEQFDKSLPTAAETNLYTKVSLPLNRYAVLQSIYAD
                     ECGRVAAAVRELAAKNPFIDMDVVQLLDSSATAFRQGSKQAKDSYGYALLQNSRQSLS
                     LVNMTIFKMLEVLDTMMSMSAGSSMESYFQMLQQMIQQQQDINEMTKRLDGQNRQQPG
                     WMEQMQALGQQQQMVRQALEELRKKYEHMQNLLGELSGLGKEMQEIEE"
BASE COUNT          639 a          567 c          675 g          486 t
ORIGIN      
        1 atgtggatgg tgaaacactc ctcgttttcc tgggcataga cgagcaggtt gaggcgcttg
       61 acgcgccgcg tgtccaggca tttcatttgg agtttcagca tccccaatac acggggttgc
      121 cggatcgttc cgagcgcaaa ctcgatggga acattctggc accaaaggga acgctcgtcc
      181 gtatgcgtgt tcagtcaaac aatccactcg aaagtgccgg catcgctttt gttggcggga
      241 gaaagtgtcc gatggagata ctggctgaat ccgtcgcttc ggcgacgttc accgttcagg
      301 actcaacaag gtatcaggtc tttctcaagg atacattcgg actggctttg accgatgggc
      361 cattctatca ggttgttgcc aaggctgact cgtcgccatc ggtttcattt gtcgcgccgg
      421 cagttcaggt gatggacgtt cccgaagaga tgaccgttcc ggtctccgga caggctgaag
      481 atgattacgg tctcaccaaa gccgagttca tctaccaggt gggctatgat caaccggaac
      541 gtgtgacgga gatgcccctc aaacctttgg caatggatgc ccagctggat tcagcaactc
      601 ccaggacagc ggagatcgcc tttgaatggg atttgagaac actcgacctg ctgcccgggg
      661 agtctgtcag ttactggttg cgcgttttcg acaatgatcc ggttgacggc ccgaaagagg
      721 ggatgtccgg cgtgcaggtc ttgcggtttc cttctctgtt cgaaatctac gaagaggtgg
      781 agaagcagga acttgcccag gtggattcaa tggatgatct tctccagaag cagcgggaga
      841 tacagaaaga gaccgatgcc ctgcgggaaa caatccaaag ggatgccgag aagccagaag
      901 aacaagttcc ttgggaacag aaagaaacgc taaaggatct caagacccga caggaacaga
      961 ttcagaagga gctggagcaa cttcagaatc tgttcctgtc gggtcagatt cagaaggaga
     1021 tggagcaact tcagcaacaa tacgatcagt ccgtgacgca gttgaaagaa gacacggcga
     1081 tttcctcaca gacatttgag aagatggcga agattcaaca actgctcgat cagatgatga
     1141 ccagcgagat gaaggagacg cttcagaaat tcaaccaggc tttggagcag atcactgcca
     1201 agcagatgga caaaagcatg ggcgatctga attcctccat gcagcagttt gaagaaagcc
     1261 ttgatcgaac cctgtctctg ctgcagcaga actacatgga acgcagcctc gaaagacttt
     1321 cttaccaggc ggaacagatg gcaaagactc agcaggaaat ccaggatcgc actcaacagc
     1381 gttcggctga agatccgctg gacgatctcg cgcgacgtca gcagcgtctt cagaaaacgg
     1441 ctgaggagtt catcaaggat gtttcggcgc ttgcacagat ggcagaacaa acggacaaca
     1501 aagagatgaa ggagaagttg gacgaagttc tcaagcaggc tgagcaaaaa gagttgcagc
     1561 aatcgctgga gcaggctcaa gagagtcttt cggaaggcaa gaaagatcag gcactgcagc
     1621 aacaacagaa agctcaggaa gcgctcgacc agatgagtgc catgatgaat gaatgcaagg
     1681 agtgcatgtc cggtggcatg agtgtcacct ttgatgtgca ggcctggcat cggcttatgg
     1741 accgggtctt tcatgtttca gagagtcagg agcagtttga caagtctttg cccaccgccg
     1801 cggaaacaaa tctctacacg aaggtcagcc tgcccctgaa ccgctatgcc gtcttgcagt
     1861 cgatctacgc ggacgaatgc ggtcgcgtgg cagcggcggt tcgcgagttg gcggcaaaga
     1921 atcccttcat tgatatggat gtggtgcaac ttctggacag tagtgcaacg gcattccgac
     1981 aagggtcaaa gcaagcgaag gattcctacg gatatgcctt gctccaaaac tcccgacaga
     2041 gcctgtctct ggtgaacatg accattttca agatgctgga agttctcgac accatgatgt
     2101 ccatgtctgc cggttcaagc atggagagtt acttccagat gttgcagcag atgatccagc
     2161 aacagcagga tatcaacgag atgaccaagc ggcttgatgg tcagaacaga cagcagcccg
     2221 gttggatgga acagatgcag gcgctcggac aacaacagca aatggttcga caggcactgg
     2281 aagaacttcg caagaagtat gagcacatgc agaaccttct tggtgagttg tccggactcg
     2341 gcaaagagat gcaggaaatc gaggaac
//
LOCUS       DAOQUC010000590         2366 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_55438_length_2366_cov_3.413241, whole genome shotgun sequence.
ACCESSION   DAOQUC010000590 DAOQUC010000000
VERSION     DAOQUC010000590.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2366
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55438_length_2366_cov_3.413241"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="PKH07_10405"
     CDS             complement(<1..230)
                     /locus_tag="PKH07_10405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35394.1"
                     /translation="MSRMFVRQSCVLSSVVCVLLLGLCFGCNKTEPSTPEPMSGASLM
                     PSSEEKPDAAPALDPSTWVAKVNDHQILESDV"
     gene            852..>2366
                     /locus_tag="PKH07_10410"
     CDS             852..>2366
                     /locus_tag="PKH07_10410"
                     /inference="COORDINATES: protein motif:HMM:NF028510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNT35395.1"
                     /translation="MRVSLRLRLTVIGVTFTILPLVILACVLYVQSHRLVVTASEESL
                     KLAYADLEHMTDGVYALCQAQNDSVLREAHHGVNTALFILKNKGQIQLSQETVVWDAK
                     NQISGQSQRVEIPKLLCGDIWPGQISDPKISVPVVDELNSVIGGTCTIFQKMNDAGDM
                     LRIATNVINKEGSRAIGTYIPVTMPDGKPNPVLAKVLKGEIFTGRAFVVDSWYFTGYA
                     PFFDASDQLVGMIYYGMKDEVTASVRAEIMKILVGETGYVFVLDSKGNYVVSKDGKRD
                     GESIWEAKDSDGNLFIQELVKISLALKPGEIGEMHYPWKNPEDPAPRMKVAKLAYFEP
                     WDWVIGVSSYEDEFLASARTVEHSGRTMIGTVIGVAVVFVALAVLAWIFLAGALARKI
                     GNVVKQLRGSSDHVSQASHQVAESSQSLAQGASEQASGIEETSASLEEITAMNSRNAD
                     NARHADGLMKETASLVHKGVDAMQRMSEAIKQIDESSAETAKILKTIDEIAFQTN"
BASE COUNT          559 a          591 c          646 g          570 t
ORIGIN      
        1 tgcacgtccg attcaagaat ctggtggtca ttgaccttgg ccacccaggt tgacggatcc
       61 aacgccggag cggcgtcggg tttctcctct gatgacggca tcaaggaagc tccagacatc
      121 ggctcagggg ttgagggctc ggtcttgttg catccaaagc acaaacccag aagcaacaca
      181 cagaccaccg acgacaagac acaagactgg cgcacaaaca tacgactcat ccttttctcc
      241 tttgggaaca aataggcgta ggaaccatcg cgaaaccaag gtgagccgta tggctcccaa
      301 gctcatgccc cgattaggca ggttagaacg ctttctcttt caaactcaag gggatattac
      361 ctgtcccagc actcactgtc aagtgcccca cactccctgt caatacagga caaaagtacg
      421 agaaatagcg ggatctgtct cacgatgact ccgggattca cggttcttgt tccgctccga
      481 ttcccagtcg agttccacca ttccaaaaga aaagagggtg gaactcgcag actcgttcca
      541 cactacacac tggttccgct tcggcagcgt cgcagagcct ctgcaaccga agttcctcat
      601 gggcctacgg cgcacctgaa ggggatgaca aatcccccct tgatcccccc ttggtaaggg
      661 gggcaggggg gattttcggg agagcatgcc tccaccaccc ctgcgtaact gagccgacgc
      721 ccctttggag caaatttcgt tgcttttgtt gtgcagaggt gtaacataaa tatgggagca
      781 gaggttttcc tctgctgggt cgcatttgtt ctgttcgggt cagagtgtgc tgcaggaaag
      841 gagtacttgc tatgcgggtc agtctacgtt tgcgtcttac ggtcatcggg gtcactttca
      901 ccattttgcc tttggtgatt ctggcgtgtg ttctctatgt gcaaagtcac cgtctcgtgg
      961 tgacagcctc agaagaatct ctcaaacttg cttatgcgga tctcgaacac atgacagacg
     1021 gtgtctacgc actgtgtcag gcgcagaatg attcggtgct gcgcgaggcg catcatggag
     1081 tcaacacggc gcttttcatc ctcaagaaca agggacagat acaactatcc caagaaacgg
     1141 ttgtgtggga cgcgaagaac cagatttctg gacagtccca gagagtggag attcctaaac
     1201 ttctttgtgg agatatctgg cctggccaga tatccgatcc aaaaatctct gttcctgttg
     1261 tggatgagct gaactcagtt atcggtggaa catgcacgat ctttcagaag atgaatgatg
     1321 cgggagatat gctgcgtatc gctaccaacg tgattaacaa agagggctct cgcgccatcg
     1381 gaacgtatat tccagtcact atgcctgacg gcaagcctaa tcctgtcctc gccaaagtct
     1441 tgaaggggga gatcttcacc ggtcgtgcct ttgttgtgga ttcctggtac ttcaccggtt
     1501 atgcgccttt tttcgatgca tccgatcaac ttgttgggat gatctactat ggcatgaagg
     1561 atgaggtgac ggccagtgtt cgcgctgaga ttatgaagat tcttgtcggt gagacgggat
     1621 atgtcttcgt actcgactcc aagggcaact atgtggtttc gaaggatggg aaacgagatg
     1681 gggagtctat ctgggaagcc aaagacagcg atgggaatct cttcatacag gaattggtca
     1741 agatttctct cgctctgaag ccaggggaga ttggtgaaat gcattatccc tggaagaatc
     1801 cagaagatcc ggcaccccgc atgaaggtgg caaaacttgc ttacttcgag ccatgggact
     1861 gggtcattgg tgtctcttct tacgaagatg agttcctcgc ttctgcgcgg acggttgagc
     1921 attccggcag aacaatgatc gggacggtca ttggcgttgc ggttgtgttt gtggcactcg
     1981 cggtgttggc gtggattttc cttgcaggtg ctcttgcacg gaagatcggc aacgtcgtca
     2041 agcagctgcg aggcagttcc gaccatgttt cccaagcgtc acaccaggtg gctgaatcct
     2101 cgcagtcgct tgctcaggga gccagtgaac aggcatccgg cattgaggaa acatccgcat
     2161 cgttggaaga gatcaccgcc atgaattcac gcaatgcgga caatgcgcgc catgcagacg
     2221 gtttgatgaa ggaaacggcc tctttagtgc ataagggcgt ggatgccatg cagcgtatgt
     2281 cagaagcgat taaacagatc gatgagtctt ctgccgagac tgccaagatt ctgaagacca
     2341 ttgatgagat tgctttccag accaac
//
LOCUS       DAOQUC010000591         2366 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_55443_length_2366_cov_3.006058, whole genome shotgun sequence.
ACCESSION   DAOQUC010000591 DAOQUC010000000
VERSION     DAOQUC010000591.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2366
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55443_length_2366_cov_3.006058"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1389)
                     /locus_tag="PKH07_10415"
     CDS             complement(<1..1389)
                     /locus_tag="PKH07_10415"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNT35396.1"
                     /translation="MKAPRFVLRFQVLLVLAALFLVPLIFSSATISTVFVKALAMNVL
                     LGCCVALEIFRLGLEARLRPLPTPLGLPVGCYLLWMGLSALFGGVHPVEVAALQRVIC
                     VVFLGYFIATCGHRLHLYRRVVMVLLSVGAITGAYAIVQILELDFFDWWRFEWSLPVR
                     RVCASFGNPTFFAGYLIPLIPLALAVRETTRRPLMRHALLALAFLDLVALGLTFSKAG
                     VLGVMAALGSWFVLRSYGRLSARSEQKVVSCFLISVGAVVLFGSVGRSLAGVDKISES
                     IVVSSGGRSQIYRSAAGMLRSNPIFGVGPGMFGHEFPKYRTPELTLFHPPHKQLLTHA
                     HCEYFEQAAELGIVGLAAFLWIVFTAIRTALAKRRSCPLAVGVVAGLVGVLVDSFFSV
                     NLRKEPNMMSFWTLIGLAICLGSRSPVVRRAQWRPALRLLSGGMALLLAFGVSFAQGR
                     DLIVDLLLRQKRA"
     gene            complement(1386..>2366)
                     /locus_tag="PKH07_10420"
     CDS             complement(1386..>2366)
                     /locus_tag="PKH07_10420"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HNT35397.1"
                     /translation="PHPVLGGLAIFIAFNLTVLADVFLLRSSLVTGDVLLRWDLTHVL
                     IGELRGHLPGVLVVLGKLHALLLGGVIVFLVGLWDDRRGLQPAWKLLWQFVAALILIR
                     ARVRFDLFPGYPLINSVLTVLWVVGLSNAFNLLDNMDGLAGGVAAIVACFFAACAYQM
                     GQIFIVIIFVAFIGSVLGFLHYNRFPAKTFMGDAGSMLIGYLIGGLGVLMTFYDFSSK
                     TSLALAKPLVILAVPAFDTLSVLAIRLKNRRPLFMGDRNHFSHRLVALGMSQKHAVLF
                     IYLVTFCTGLGALLIRKLDVWGAMVILLQVLIIFAIIALLERVRTRDNQP"
BASE COUNT          686 a          679 c          614 g          387 t
ORIGIN      
        1 agcccttttc tgccgcagca ataagtccac tatcagatca cgtccctggg cgaaggaaac
       61 gccaaaggcc agaagaagcg ccatcccccc cgacagaagt cgtagcgcag gacgccattg
      121 cgctcttcga acgacgggac tccttgagcc aagacagatc gctaggccga tgagcgtcca
      181 aaaactcatc atattgggtt ccttccgtag gttcacggag aagaaactgt caaccaaaac
      241 cccaacaagt ccagcaacaa cgcccacggc gagggggcag ctgcgacgct tggcgagtgc
      301 agttcgaatt gccgtgaaca cgatccaaag aaaggcggcc aggccgacga tccccagttc
      361 tgcagcctgc tcaaagtact cgcagtgagc gtgagtcaaa agttgtttgt gtggcggatg
      421 aaacagggtc aactctggag ttcgatattt cgggaattca tgaccgaaca tcccaggccc
      481 aacgccgaag atggggttcg aacgaagcat tccggcagcg cttcgataga tctgacttct
      541 gccccctgag gagactacga tcgactcgga gatcttgtca acgcctgcca gactccgtcc
      601 aacagagcca aacagtacaa ccgcaccaac cgagatgaga aagcatgaca ccactttttg
      661 ttcagaacgt gccgacaggc ggccgtatga tcggagaaca aaccaggagc ccagcgctgc
      721 catcacaccc aacactccag ccttgctgaa agtaagtccc aaagccacca agtccaaaaa
      781 cgccagagcc aggagtgcat ggcgcattaa gggtcttctc gttgtctctc gaacggcaag
      841 ggcaagcggg atcagcggaa tcagatatcc ggcaaaaaaa gtcggatttc caaaggaggc
      901 gcacactcgg cgcaccggca gcgaccattc aaaacgccac cagtcgaaga agtccagctc
      961 gagtatctgc acgatggcat aggcgcccgt aatcgcgcca acactcaaaa gcaccatcac
     1021 aacccgccgg tacaggtgca gacggtgacc acaagttgcg atgaaatagc cgagaaacac
     1081 aacgcagatt actcgctgaa gcgcggccac ttccactgga tgaacgcctc cgaagagcgc
     1141 tgaaagcccc atccagagaa gatagcatcc gaccggcagt ccaaggggag tgggcagtgg
     1201 tcgcagcctg gcctcaagcc ccagtcggaa tatctccaat gcaacgcagc aacccagcag
     1261 cacgttcatc gccaatgctt tgacaaagac ggtgctgatc gtggcgctgc tgaagatcaa
     1321 cggaaccagg aacaatgcag ccagcacgag taacacctga aaacgtaaga caaatcgcgg
     1381 tgctttcatg gttggttatc ccgggttctg actcgttcca gcaaggcaat gatggcaaag
     1441 attatcagca cttgaagcag aatcaccatc gcaccccaaa cgtcgagttt tcgaatgagg
     1501 agtgctccca gacccgtaca aaaggtcacc agatagatga acagaacggc gtgtttctgt
     1561 gacattccca aagccacgag ccgatgcgag aagtgatttc tgtctcccat aaacaagggg
     1621 cggcggttct tgagtcggat cgccaggaca gaaagggtgt caaaagcggg tacggcaagg
     1681 atcaccaacg gtttggcaag tgccagcgaa gtctttgaac tgaagtcata gaatgtcatc
     1741 agcacgccaa gccccccaat tagatatccg atcagcatgg aacctgcgtc acccatgaag
     1801 gtctttgccg gaaagcggtt atagtgcaga aaaccgagca ccgatccgat gaacgccaca
     1861 aagatgatga caataaatat ctgtcccatc tggtacgcac aggccgcaaa aaaacacgcc
     1921 acaattgcgg caacaccacc cgccagacca tccatattat caagcagatt gaaggcatta
     1981 ctcagaccaa ccacccacaa aacggtcagg accgagttga taaggggata gcctggaaag
     2041 agatcaaagc gaactcgcgc gcggattagg atcagtgccg cgacaaactg ccacagcagt
     2101 ttccaggcag gttggagccc ccgtcgatcg tcccaaagtc ccaccagaaa gacgatcacg
     2161 ccaccgagca agagcgcatg gagtttgccg agcaccacca acactccagg aagatgacct
     2221 cggagttcgc cgataaggac gtgcgtcaga tcccaccgca gcaacacatc tcctgtcaca
     2281 agactggagc ggagcaagaa gacatcggcc aagacggtca ggttgaatgc gatgaagatg
     2341 gcgagtccac ccagcactgg atgcgg
//
LOCUS       DAOQUC010000592         2365 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_55498_length_2365_cov_2.438528, whole genome shotgun sequence.
ACCESSION   DAOQUC010000592 DAOQUC010000000
VERSION     DAOQUC010000592.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55498_length_2365_cov_2.438528"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /locus_tag="PKH07_10425"
     CDS             complement(<1..254)
                     /locus_tag="PKH07_10425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HNT35398.1"
                     /translation="MNLLLLSLGLLLAGSFFSLFLGRSSQARTLGAATSILGCVAGIV
                     PACSVLLGGLTETFASAWEIPMGIFAVRLDPLSAFFVLTIL"
     gene            complement(552..>2365)
                     /locus_tag="PKH07_10430"
     CDS             complement(552..>2365)
                     /locus_tag="PKH07_10430"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HNT35399.1"
                     /translation="ERFLMNRCLFALMSLLLALPCFAGEWPGCDLSGAPLQEYRAEDE
                     VPLQQFAIAGSTILESSGLRPANMEWVNETLANMTLDQKIGQMIMIWYSSGTSLSDVV
                     QYHVNGCIFSSANISGAAAIVSAVNQLQAAATTPLWFAADSECGMGARFGDATVFPMN
                     MAQGAADSEILAASQGWVTARESWAMGIQIGFGPVVDVNTDPANPIIATRSYGDDHTQ
                     VARLAHAYIEGANGAGMLTCPKHYPGHGPTDLDSHLALPVVNLPESELRRVHIYPYQQ
                     LIAQGFSDLVMSAHIWFTALDPGASPWPATLSSKAMTDILRTDLGFQGVSITDAFTMA
                     GVSDVLEPAEAVVVAVSNGMDIILMPPGLDLTFNALKAAVLDGTLSEDRINTAVRRIL
                     TAKSRVWLPDFPYRDGTAVGTILKSPEHLAVSEAVARASVTQFWERGGFVPIQPEEDV
                     LCLRLVDNGIIFNVPARPSTHFTDRLDAALPNFNLVNVARTLRTRDINDLVALASGYD
                     KVIVACYDWKPIMSDNQVTLVNRLLSDSMPVIFISFGSPYHALKLDGLQSFYCAYANA
                     ESSQRAGADVLLGNLVAVGKPPVSTYQEAPAPFWSLY"
BASE COUNT          615 a          632 c          631 g          487 t
ORIGIN      
        1 agaatcgtaa gaacaaaaaa agcagagagg gggtcgagcc tgaccgcaaa gattcccatc
       61 ggaatctccc aagcggaagc aaaggtctca gtgagtccac ccagcaaaac ggaacaggca
      121 ggaacgattc cagcaacaca tccgagaatg ctcgtcgccg cgcccagagt acgagcctgt
      181 gacgatcttc ccagaaaaag actgaagaag gaaccagcca gcagaagacc aagagagaga
      241 agaagcagat tcacctcagt ctcccttctt caaaagagag acatcggtgg acgcgagaga
      301 caaaaaggag atttgtgaag aatgacaaac cttcatgcct ggcgtatggg cagccttcat
      361 catcagacgg acttgtcatc ttcgcggttc tcagtagtca tgcccacacg aagcagacca
      421 cattgaaccc aatcctttct cgatatttga gagtacggtc acttctagcg aagctgcatt
      481 cgcttgtcaa gatagagtct caagaaaacg gtaatcttca tcaacgatat ccccctgtat
      541 ggcatctttg ttcagtagag gctccaaaac ggcgcagggg cttcctgata ggtggatact
      601 ggcggtttgc cgactgcaac cagatttccc agaagcacat ccgctccagc tctttgcgat
      661 gattccgcgt ttgcataggc gcaatagaaa gactgcaaac catcaagttt cagggcatga
      721 tacggacttc caaatgagat gaagattacg ggcatcgaat ccgacagcag ccgattcact
      781 agggtaacct ggttgtcact catgattggc ttccagtcat agcaggccac aatgactttg
      841 tcatatccgg aggcaagtgc cacgagatcg ttgatgtcac gcgttctgag agtgcgtgcg
      901 acattcacca agttaaaatt cggcaaagcc gcgtcgagcc tatctgtgaa atgggtcgag
      961 ggacgcgcag gcacattaaa gatgattcca ttgtctacaa gtctgagaca gaggacgtct
     1021 tcctcgggtt gaatgggaac gaatccgcct ctttcccaaa actgcgtgac tgatgcgcga
     1081 gcgacggctt cggagacggc gagatgctcc gggcttttca gtattgttcc cacagcagta
     1141 ccatcgcgat agggaaaatc ggggagccag acgcggctct tggcagtcag aatccttcgg
     1201 actgccgtgt tgattcgatc ctcagagagg gttccgtcca atacagcggc cttgagggca
     1261 ttgaatgtca agtccaggcc gggtggcatc agaatgatgt ccattccatt gctcacagca
     1321 accaccactg cttccgctgg ctcgagcaca tccgagacac ccgccatcgt gaaggcgtca
     1381 gtaatgctca cgccctgaaa acccaagtca gttctgagga tgtccgtcat ggcttttgac
     1441 gaaagtgtcg caggccaggg acttgcacct ggatcgagtg cggtaaacca gatgtgggcg
     1501 gacatgacca agtccgaaaa accctgagcg atcagctgct ggtagggata gatatgaact
     1561 cgacgcaact ccgactctgg cagattcacg accggcagag cgagatggga atcaaggtca
     1621 gtcggcccat gccctggata gtgcttcgga caagtcaaca tgccggctcc gttggctccc
     1681 tcaatgtacg catgtgccag tcgggcgacc tgtgtgtggt cgtctccgta ggaacgggtg
     1741 gcaatgattg gattggccgg atcggtattg acatctacta caggtccaaa accgatttgg
     1801 atacccattg cccacgattc tcgggctgtt acccatcctt gagaggcggc gagaatctca
     1861 ctgtcggcag cgccttgagc catgttcatt ggaaagaccg tcgcatctcc gaatcgagcg
     1921 cccatcccac actccgaatc tgctgcaaac cagaggggag tcgttgcggc ggcctgtaac
     1981 tggttgaccg ccgagacgat tgcggcagcg cccgagatgt tggcgctgct gaaaatgcat
     2041 ccattgacat ggtactgaac cacatcggac agtgacgtac cggatgaata ccagatcatg
     2101 atcatctgtc cgatcttctg atcgagcgtc atattggcca gcgtttcgtt gacccactcc
     2161 atatttgccg gtcggagacc ggaggattcg agaatcgttg aaccggcgat cgcaaactgc
     2221 tgaagcggca cctcatcctc agcgcggtat tcctgcaaag gcgctccgga cagatcacaa
     2281 cctggccact caccggcaaa gcaaggcagc gccaacagaa gactcatcag ggcaaaaaga
     2341 catcggttca ttagaaaacg ctcca
//
LOCUS       DAOQUC010000593         2361 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_55632_length_2361_cov_3.561578, whole genome shotgun sequence.
ACCESSION   DAOQUC010000593 DAOQUC010000000
VERSION     DAOQUC010000593.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2361
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55632_length_2361_cov_3.561578"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..784
                     /locus_tag="PKH07_10435"
     CDS             <1..784
                     /locus_tag="PKH07_10435"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNT35400.1"
                     /translation="AMLAAEHNLHAFVEKPLAMKASEADQIVQAFRKSGKILMVGHVV
                     RFFGEYVQARHRVLSGAIGSPVMIRTTRAGRMPRGVGNWFGNYAQSGGVILDLIVHDL
                     DYAVWCFGAPRRVYAKSVVSDVNAVGVSLDHAFVTVKFQNGVIGHFEGSWALPDGFYT
                     AFEVSGSGGIIQYDSRKVSPLLLSESAAEEGQKPNVFLPESPLHDCPYRSEVQHFAEV
                     LQGKAELVVKPEDAVTCVAVAASAWWSLRTGQSVQIQGGAKS"
     gene            781..1770
                     /locus_tag="PKH07_10440"
     CDS             781..1770
                     /locus_tag="PKH07_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017251125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNT35401.1"
                     /translation="MKVVLLSFAHLHMGSYAKQLNAHPDVEIVGIYNEDDSFGQKMAD
                     QYRTGFFKTLDEALAVPSDAVVVCSANADHKPMVVAAAQAGKHVMCEKPIATTIADAQ
                     AMIDACVANRVKLMTAFPCRFIPAVERGKRLIEEGRLGKILAIRATNHGSMPGGWFAE
                     KEKAGGGAVMDHTVHVADLIRWMLGLEIVKVYAEVDNLYYPELNIDDVGQLNMTLSDG
                     SFVTLDTSWSRRRPFPFWGDVTLEFVGERGVLEIDSFAQKIDVYSAVNKSVSWDYWGS
                     DMDKALVADFIRMVTKEEPAFISGEDGLKALEVAVAAYRSGETGEVVNLPLEN"
     gene            complement(1823..>2361)
                     /locus_tag="PKH07_10445"
     CDS             complement(1823..>2361)
                     /locus_tag="PKH07_10445"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNT35402.1"
                     /translation="TGEIKVAGLDLTRLTSKQLAYFRGNHIGYVFQSYNLLPAYKAVA
                     NVAMPLLFSGEPYERAVSRAEEVLTRVGLGHRLTHRPDELSGGQQQRVAIARALANRP
                     AIILADEPTGNLDLHTGEEIIHLLRQLCLEDGVTVVTATHDHKMLAASDRILWIKDGA
                     VERLQKRSEIDIRVGTVK"
BASE COUNT          501 a          564 c          715 g          581 t
ORIGIN      
        1 tgccatgctt gccgcggagc ataatcttca tgcgtttgtg gagaaacctc tcgctatgaa
       61 ggccagtgag gcggatcaga tcgtgcaggc ttttcgcaag tcgggcaaga ttcttatggt
      121 cggtcacgtg gttcgcttct ttggcgagta tgtgcaggcg agacatcgcg tcctctcagg
      181 tgccatcggg agtcccgtca tgatacgaac gacgagagcg ggtcggatgc caaggggcgt
      241 tggcaattgg tttggaaact acgctcagag tggcggtgtt atactggatc tgatcgtgca
      301 tgatttggac tatgcggttt ggtgtttcgg cgcgcctcgt cgggtctatg ccaagtcggt
      361 ggtttcagat gtgaacgccg ttggtgtttc tttggatcac gccttcgtca cagtcaaatt
      421 ccagaatggg gtgatcggtc actttgaagg gagttgggct cttccagacg gattctacac
      481 agcatttgaa gtgtcaggtt ctggaggaat catccagtac gacagccgaa aggtgtctcc
      541 tctgttgctt tcggaatcgg ctgccgagga agggcagaaa cccaatgtct ttctaccgga
      601 gagtcctttg catgactgcc cttacagatc agaggttcag catttcgcag aagtgctgca
      661 gggcaaggcg gaacttgtgg tcaaacccga agatgcagtg acttgcgttg ctgtggcagc
      721 aagtgcctgg tggtctctgc ggactggaca gtcggttcag attcagggag gtgcgaagtc
      781 atgaaagtcg ttctactgag ttttgcccac cttcacatgg gcagctacgc gaaacagttg
      841 aatgctcatc ccgatgtcga gattgttggg atatacaacg aagacgattc ttttggacag
      901 aagatggcag accaatatcg aacgggattc ttcaagacgc tggatgaagc gcttgcggtt
      961 ccctccgatg ccgtggtggt ctgctcggcc aatgctgatc acaaacccat ggtggtggca
     1021 gccgctcagg ccggcaaaca tgttatgtgt gaaaagccca ttgccacgac cattgcggac
     1081 gctcaggcga tgattgatgc ctgcgtggcc aaccgggtga aactgatgac ggcttttcct
     1141 tgtcgcttca tccctgcggt cgagcgaggc aaacgcctca ttgaggaggg ccgtctcgga
     1201 aagattctgg cgattcgtgc taccaaccac ggttccatgc cagggggatg gtttgccgag
     1261 aaagaaaagg ccggtggggg tgcagtcatg gatcatacgg ttcatgttgc cgacctcatc
     1321 cgttggatgt tgggtctgga gatcgtcaag gtctatgccg aggtagacaa cctctattat
     1381 cctgagctga acattgacga cgttggccaa ctgaacatga ctctttccga cgggagtttc
     1441 gtgactctgg acaccagttg gtctcgccga agacctttcc cattctgggg tgacgtcacc
     1501 ttggagttcg ttggagagcg aggtgttttg gagattgact cgttcgctca gaagatagac
     1561 gtctactcag ctgtgaacaa atctgtcagt tgggactatt ggggcagcga catggacaaa
     1621 gccctggtgg cggatttcat ccgaatggta acgaaggagg aaccggcgtt tatttcgggt
     1681 gaagatggtc tcaaagcatt ggaagttgct gttgctgctt accgctccgg tgagacgggt
     1741 gaagtcgtga atctgccgct tgagaactga ggacgggcag ggataggcca atgcccctgc
     1801 agtggtacga agaacccgtc cgttatttga cggtgccgac gcgaatgtca atctcggatc
     1861 gtttctggag tctttccact gcgccgtctt tgatccagag aatgcggtcg gacgctgcga
     1921 gcatcttgtg atcatgcgtt gcggtgacta cggtaacgcc atcctcaagg cacaattgcc
     1981 tgagtagatg gatgatctct tccccggtgt gcaaatccag gtttccagtc ggttcatccg
     2041 cgagaatgat ggcaggacga tttgccaaag ccctggcaat cgcaacacgc tgctgctgcc
     2101 ctcccgacag ttcatccggg cggtgagtca aacgatgtcc gagcccaaca cgggtcagca
     2161 cctcttccgc tcgggagacg gcacgttcat agggctctcc gctgaacaag agtggcatgg
     2221 cgacgtttgc tacagctttg taggcgggga gcaggttgta tgactgaaag acatacccga
     2281 tgtggttgcc tctgaagtaa gcaagttgtt tgctggtcag gcgtgtaaga tcaagacctg
     2341 cgactttgat ctctccagtg c
//
LOCUS       DAOQUC010000594         2358 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_55761_length_2358_cov_3.065567, whole genome shotgun sequence.
ACCESSION   DAOQUC010000594 DAOQUC010000000
VERSION     DAOQUC010000594.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2358
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55761_length_2358_cov_3.065567"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..353
                     /locus_tag="PKH07_10450"
     CDS             <1..353
                     /locus_tag="PKH07_10450"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HNT35403.1"
                     /translation="DLGGMVRSGTPTFSQIIECYGKIIERIQEGAPDARIHVMSCFPT
                     RDKYAIIAPQVRQFNREIKRIAQEKKVDYVDVYSKTVGRKSLMKLEYSQDGLHVNAKG
                     YQILAEAIQSRLRL"
     gene            complement(372..1637)
                     /locus_tag="PKH07_10455"
     CDS             complement(372..1637)
                     /locus_tag="PKH07_10455"
                     /inference="COORDINATES: protein motif:HMM:NF024120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HNT35404.1"
                     /translation="MFLPRWILCFVLVVLVGGSGWCVPQSANVRDFGAKGDGLTDDTQ
                     AIQKALAAISSGGVVTLPAGAYIVSAALNVPHGGTLIGEGVRWENGTSQLYVLENGFS
                     PIVLDHGASVKGLIIIYPNNMDNSNPTPYPPSILLNGINPSVENIVFSGAWIGVSTPQ
                     GGGNAGQGLFKELTGFVHHVGIHLDESRDVNRIQDVHWFVGGKDTPGKPSYYRHNRVG
                     FEFGSVDGIIMERCFIIGGKTFFHQLPEKTLADGTKRGTHSLGHHIDQCWIEDVDYGF
                     IFEGATGFVLSSANILIRKNGIGVKVTPQSLFYNDVIDSVQVRSFGEPIVGFEYGCLT
                     PHSRNRLSIADCQVVDGSPALHLMSGAVRANIHDSHFQAVPVCPAIKIDRGADLFTIA
                     NNILSAPRAIQDNSGRRARKTIKGNLHER"
     gene            complement(1725..>2358)
                     /locus_tag="PKH07_10460"
     CDS             complement(1725..>2358)
                     /locus_tag="PKH07_10460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009324471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNT35405.1"
                     /translation="KGAGTRCLVDCPIEPGCLYSAYKHYINHPSRWSFYVWDTLEHIS
                     NPTIEQKVESLKTTNPYGRCVWRCDNDVVDHQSIVVEFSDGSTSTLNMIGGTSKPLRS
                     IHLLGTTGEIQGVINDSKFVIRHFDTSPGHEYTEEEVDLNIQGDKTGAFGGHGGGDLR
                     LVADFVQTLRGEPRSISCTSLEDSVYGHLLGFRADQSMQEHKVVEIPAVH"
BASE COUNT          556 a          614 c          619 g          569 t
ORIGIN      
        1 atgatctcgg tgggatggtc cgatcaggaa cgccgacgtt ttcacagatt atcgagtgct
       61 atgggaagat catagagagg atccaggaag gggcacccga tgcacgaatt catgtcatgt
      121 cctgttttcc aactcgcgac aagtacgcga tcatcgcgcc tcaagtacga cagttcaatc
      181 gagagatcaa gagaattgct caagagaaga aggtggacta tgttgatgtc tacagcaaga
      241 cggttgggcg caagtctctc atgaagttgg agtactcgca ggacggtctt cacgtcaatg
      301 cgaagggcta ccaaatcctt gccgaggcca tccagagccg actgaggctt taggacagag
      361 ggcgattcgg atcatcgttc atgcagattt cctttgattg ttttccgtgc ccttctgccc
      421 gagttgtctt ggattgctct tggtgcagag aggatgttgt ttgctatcgt gaagagatct
      481 gctcccctgt caatcttgat ggccggacag actgggactg cctgaaaatg gctgtcatgg
      541 atatttgccc gaacggcacc cgacatcagg tgaagtgcgg gtgatccgtc cactacctga
      601 cagtccgcaa tgctcagtcg gttgcggctg tgtggtgtca gacagccgta ttcaaaacct
      661 acgattggct ctccaaagga acgcacttga acgctgtcaa tgacatcatt gtagaataaa
      721 ctttgaggag tgaccttcac gccgatgcca ttcttgcgaa tcaagatatt ggcactggag
      781 agcacgaatc ccgtagctcc ttcaaagatg aacccgtagt ccacatcctc tatccaacac
      841 tgatcgatat ggtgacccag tgaatgggtg cctcgttttg ttccatcggc aagggtcttc
      901 tcagggagtt ggtggaagaa ggtcttccca ccaatgatga aacagcgctc catgatgatg
      961 ccatctacgc tgccgaactc gaatccgacg cggttgtgtc tgtagtagga aggcttgcct
     1021 ggagtgtcct tgcctccaac aaaccagtga acgtcctgaa tgcgattcac atcacggctt
     1081 tcgtcaagat gaatacccac gtgatgcacg aatcctgtca gttccttgaa aagtccctga
     1141 ccggcatttc caccaccctg tggtgtggag acaccgatcc aagcaccgct gaacacgatg
     1201 ttctccacac tcgggttgat cccgttgaga aggatagagg gtggataggg tgttgggttt
     1261 gagttgtcca tgttgtttgg atagatgatg atcaggccct tgacgctcgc accgtggtcg
     1321 agaacgatgg gtgaaaatcc gttttcgaga acgtacagtt gcgatgtgcc gttctcccat
     1381 cgaacgcctt ctccgatcag cgttccgcca tgagggacat tcagggcggc agagacgata
     1441 taggcacctg caggaagagt caccacgccc ccactcgaaa tggctgccaa ggccttctga
     1501 atggcttggg tgtcatcggt cagtccgtca cccttcgcgc caaaatccct gacgttggca
     1561 ctctggggga cgcaccagcc actcccacct accaggacaa caagaacaaa gcacagaatc
     1621 catcttggca aaaacatctt cagttgctcc tttcacgtcc attcagtccg agctggcatc
     1681 gcatctgtca aacagatcct atccattaga accactgctg ctgatcagtg tacagcgggt
     1741 atttcaacaa ctttgtgttc ctgcattgac tgatcggctc gaaaccccag caaatgaccg
     1801 tacacagagt cttcgagact agtgcaggag atggagcgtg gttctcccct gagcgtttgc
     1861 acgaagtcag caaccaaccg cagatcgcct ccaccatgac cgccgaaggc tcctgtcttg
     1921 tctccctgga tattgagatc cacttcctcc tcggtatact cgtgtccagg actggtgtcg
     1981 aaatgcctga tgacaaactt gctgtcgttg atgactcctt ggatttcgcc tgttgttccg
     2041 agcagatgga tggagcgcag tggcttggac gtgccaccga tcatattcaa ggtcgaggta
     2101 gagccgtcgg agaattccac gacgattgac tgatgatcca ccacatcatt gtcacatctc
     2161 cagacgcaac gaccatacgg gtttgtcgtc ttgagcgatt caaccttctg ctcaatggtc
     2221 gggttggaaa tgtgttccaa tgtgtcccag acgtagaaag accagcgcga aggatggttg
     2281 atgtagtgct tgtaggcgga atagaggcat ccaggttcga tgggacagtc cacgagacat
     2341 ctcgtacctg ctcccttg
//
LOCUS       DAOQUC010000595         2355 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_55880_length_2355_cov_2.503043, whole genome shotgun sequence.
ACCESSION   DAOQUC010000595 DAOQUC010000000
VERSION     DAOQUC010000595.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2355
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55880_length_2355_cov_2.503043"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..173
                     /locus_tag="PKH07_10465"
     CDS             <1..173
                     /locus_tag="PKH07_10465"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF014867.2"
                     /note="Catalyzes the reversible oxidation of malate to
                     oxaloacetate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HNT35406.1"
                     /translation="LNGEYGLSDLYLGVPVKLGAGGVEEVVSLNLTVEERSMLRQSAE
                     VYKESISHLTSV"
     gene            314..970
                     /locus_tag="PKH07_10470"
     CDS             314..970
                     /locus_tag="PKH07_10470"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNT35407.1"
                     /translation="MKLQWLGHSCFLIESSEGVRVITDPYKAGAFGGALSLGKIHDRA
                     DVVTVSHEHDDHNAVGQVPGNPLIMRASGSAKGVDFHVLDVFHDTTQGSERGRNRIFQ
                     FAIDGIGIVHYGDLGHDLSASQTAHLRGWSADVLLIPVGGFYTIDAAAADRLSDALAP
                     SIVIPMHVKHAKVNFPIAPVEEFLKRRKQVKRVGSSCVEITSDSLPATSETWVLEPAM
                     "
     gene            1107..>2355
                     /locus_tag="PKH07_10475"
     CDS             1107..>2355
                     /locus_tag="PKH07_10475"
                     /inference="COORDINATES: protein motif:HMM:NF028511.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HNT35408.1"
                     /translation="MKHVTTIRFRITVWFVMIILIANASLSFVTVVHIGKITTQEVQK
                     RVRFNLNSARGVYQYHVERISTLLQGAAIRFSPVQTPDGAVDPLLKEVLRSLLEENRL
                     DMLTVMDANGIVLYRAHNSESQGDSLRDNAVVSLAIESNKPSQGTIVLSREQLEREHF
                     QLAERARLQVETTPAARSLRESVQSEGMVIAAAVPFLTLAHGEERRGMIYGAILLNRR
                     HEIVDRIREELFEKDKQGKKETGTATLFQGDLRISTNVLKSDGTRAVGTQMSSEVYQQ
                     VILKGQLWSKRAFVVNDWYLAAYEPIRDPRGQIIGALYVGLLEEPFIRPMRLLASVFV
                     SMVALTTLLSLVLLTFVIRHVLSPIHTIVTMSARVIEGDLSARVGVRPPGEMGLLCET
                     IDKMADAVSLREERLQKATREQIG"
BASE COUNT          510 a          561 c          695 g          589 t
ORIGIN      
        1 ttctcaacgg agagtacggg ttgagtgatc tctatcttgg tgtaccggtc aaactcggcg
       61 ctgggggcgt tgaagaggtg gtctctctga atctgaccgt tgaagagcgt tccatgctcc
      121 gacagtctgc agaagtctat aaagagagca tcagccatct gacatcggtc tagtctgatt
      181 tgagaaacgt ctgtttcatc tcccctttgc aggggagttc tgcaacggtg actcaggatc
      241 ggttggctgg tgcggtgctg gaaacggtct tcttcagagc agattccaga agggcaatgg
      301 aggatgagaa acaatgaaac tccaatggtt ggggcattcg tgttttttga ttgagtcctc
      361 ggaaggggtt cgtgtcatca ctgaccccta caaggctggg gctttcgggg gggcgttgtc
      421 acttggcaag atccatgacc gtgcagatgt ggtgacggtc agccatgagc atgacgacca
      481 caatgctgta ggtcaggtgc ccggaaatcc tctgataatg cgtgcttccg ggagtgccaa
      541 gggtgttgat tttcatgtgc tggacgtctt tcatgacacc acgcagggta gcgagcgagg
      601 cagaaatcga atcttccagt ttgcgattga cgggatcggt atcgttcatt atggtgatct
      661 cggacacgac ctatccgctt cccagacagc ccacttgagg ggatggagtg ctgatgttct
      721 tctgattcct gttgggggat tctataccat tgacgctgca gcggcggatc gtttgtcaga
      781 tgccctggca ccttcgatcg taatccccat gcacgtcaag catgcgaagg tcaactttcc
      841 catcgcgcct gtggaggagt ttctgaaacg cagaaagcag gtgaagagag tgggttcttc
      901 ttgcgtcgag ataaccagtg atagcctgcc tgcaacttcg gaaacgtggg tgcttgagcc
      961 tgcgatgtag gctccgtatg ggctgcccgg tttggagcga tccttcccga tccttgggca
     1021 tgacgttggg cgagtgttgt tgcagaggct gagcaggcga gacaaactgc tgttcaagcg
     1081 atagaagact catggcgtat acggatatga aacatgttac tacaatacgt tttcggatca
     1141 ctgtatggtt tgtgatgatc attctcatcg cgaatgcctc actttcgttt gtcacggtcg
     1201 ttcacattgg caaaatcacc acgcaggaag tgcagaagcg tgttcgcttc aacctgaact
     1261 ccgctcgagg tgtttatcaa taccatgttg agcgcatctc aactcttctt cagggcgcgg
     1321 cgatccgatt ctcacctgtt cagactccgg atggagcggt ggatccgctc ctgaaagaag
     1381 tcctgagaag tcttctggaa gagaaccgcc tggatatgct gacggtgatg gacgccaacg
     1441 gaattgttct ttaccgcgca cacaactccg agtcccaagg ggacagtctt cgtgacaatg
     1501 cggtggtgtc cttggccatc gaatcgaaca aaccatcgca gggcacgatt gttctgagcc
     1561 gagagcaact ggagcgtgaa cacttccaac tggcggagcg tgcgcgtctg caagtggaga
     1621 ccacccccgc cgcgcgatca ctgcgggaat ccgttcagag tgagggaatg gtcattgccg
     1681 ccgcagtgcc gttcttgact ctcgctcatg gcgaagagag acgtgggatg atctatggag
     1741 ccattctgtt gaatcgccgc catgagattg tggatcgaat tcgagaggaa ctttttgaga
     1801 aggacaagca ggggaagaaa gaaactggaa cagcgacttt gttccagggc gatctgcgca
     1861 tttcgaccaa tgttctcaag tctgacggaa cacgcgctgt tggcactcag atgagttcag
     1921 aagtctacca acaggtcatt ctcaaggggc agttgtggtc aaagagagct tttgtcgtca
     1981 acgactggta ccttgcggcc tatgagccaa ttcgtgatcc cagaggtcag attatcggtg
     2041 ccctctacgt tggcctgctt gaggaaccct ttatcagacc gatgagattg ctggcttcgg
     2101 tgtttgtcag catggttgct ctgacgactc tgttgagcct ggtgctcctg acttttgtta
     2161 tccgtcatgt gttgagtccg attcatacca ttgtcaccat gtcagccagg gtgatcgagg
     2221 gcgatctttc ggctcgtgtc ggagtgcgtc cgcccggaga gatggggctg ctgtgtgaga
     2281 ccattgacaa gatggcggat gcggtttctc ttcgcgagga gcgacttcag aaggccaccc
     2341 gagaacagat tgggc
//
LOCUS       DAOQUC010000596         2353 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_55931_length_2353_cov_3.595300, whole genome shotgun sequence.
ACCESSION   DAOQUC010000596 DAOQUC010000000
VERSION     DAOQUC010000596.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2353
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55931_length_2353_cov_3.595300"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2353)
                     /locus_tag="PKH07_10480"
     CDS             complement(<1..>2353)
                     /locus_tag="PKH07_10480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35409.1"
                     /translation="GVNPGFSMNPGDSFLPQFALVDEDLNTPTYMNPLVDEDLKDYYV
                     WVDENGNGVFDIQYTDLDGDNDFHLDEDINGNGIFEQETEDFDGDGRWDRGYSATGDE
                     IYFDYNGNGLYDYRVGHPQSGVDVYECVFPGTDGIISMTDGQPYEQWISLDDDGDGEQ
                     GSPLSPNLRWMPGLEAYRGGVKMADGLDNNGNGEDGSPIDPITGFKMADGLDNDGDGE
                     FCSPICPVTGLKMADGIDNDLNGLVDEGIDEGIDEGINEDLGDFAFAPLTPWIESSFG
                     SGFALGDLDGMYLYRDDGDGLFDPTKDTPASRNDSEQVDWRWLSQPLDSFVDLDPVTG
                     QPDWLKASLMYPKPPYYTAVIEVQSDEAEAIPRTDDRTFDFFAVLTTDGDDWGDITSG
                     NARFDATGMRYGDDFMVGLARNAIRLDKGVDEGFSVPNPPDTALPNMKKLTKPLFGML
                     YIGDIVGYKPWYSQGEHLEEGGYGGSLPFIGNPYVDATSSPIGVIGINAADAPQGDAG
                     NDQLENVRVRFQNLTYDSYNESVPEGTFDIDVQQGYENVTSATPVYVKDLMRLADDST
                     GGVGLYVDNPDAGIQGVFDEMDIPVPLRNTKEAPLTFRMEHDGSIYVDLNSIAGLDVP
                     GDDQTAKTKGFDFFVVVRTSDEIDYRDTFKIWIDDGDVRFRSGVSVENTRREVHPIAA
                     NVPVFFRDLVEPGQTITAESEPTAVVGINLYDRTLSNSGSPAKLEWLRIRFLNVGNDN
                     DFTNSDLMPMSLDESSGVALYRDKAPDGDDSNGIEKLGVFDETD"
BASE COUNT          532 a          700 c          557 g          564 t
ORIGIN      
        1 gatcggtctc atcgaagaca ccaagtttct cgataccgtt gctgtcatcc ccgtcgggtg
       61 ccttgtcccg gtacagcgcc acaccgctgc tctcgtcaag gctcatcggc atgagatccg
      121 aattggtgaa gtcgttatca tttccaacat tcaagaatcg gatgcggagc cattccagtt
      181 tcgccgggct gccagagttg gaaagcgtcc gatcgtaaag gttgatcccc acaacagccg
      241 tcggctcgga ctctgcagtg atcgtctgac ccggttcaac cagatcgcgg aagaacactg
      301 ggacatttgc ggcaatcgga tgcacttccc gccgagtgtt ctcaacggat acaccacttc
      361 ggaatcggac gtctccgtcg tctatccaga ttttgaaggt gtctcgatag tcaatctcat
      421 cactggtgcg aaccaccaca aagaaatcaa accccttggt ctttgcagtc tggtcgtctc
      481 ctgggacatc cagaccggcg atcgaattca ggtcaacata gatgcttcca tcatgctcca
      541 tgcggaatgt cagcggtgct tcctttgtat tgcgcagtgg aaccggaatg tccatctcat
      601 caaacacacc ctgaatacct gcatcggggt tatccacata gagccccaca ccccctgtcg
      661 aatcgtcagc aagacgcatc agatccttga cataaacagg ggtggcgctg gtgacgtttt
      721 catatccctg ctgaacatca atgtcgaacg tgccttcggg cacagattcg ttgtaggaat
      781 cgtaggtcag attctggaaa cgaacacgaa cgttctccag ttgatcgttg ccagcatctc
      841 cctgaggcgc atctgctgca ttgataccaa tgactccgat cggcgagctg gtcgcatcca
      901 catacggatt gccaatgaac ggcagcgaac cgccatagcc accctcttcc agatgctctc
      961 cctgcgaata ccacggcttg taaccgacaa tatccccaat gtagagcatg ccaaagagag
     1021 gcttggtcag ttttttcatg ttcggaagtg ccgtatccgg aggattcgga acactgaatc
     1081 cttcgtcaac acccttgtcg agtctgatgg catttcgtgc cagacccacc atgaaatcat
     1141 caccatagcg catacccgtt gcatcaaagc gtgcgttgcc actggtgata tcgccccagt
     1201 catccccgtc tgtcgtcaaa actgcaaaga agtcgaaagt cctgtcgtct gttctgggaa
     1261 ttgcctctgc ttcatcggac tgcacttcaa tcacagccgt atagtagggc ggtttgggat
     1321 acatcaacga agcttttagc cagtcgggct gccctgtcac cggatccagg tcaacaaaac
     1381 tgtccaacgg ttggctgagc catctccagt caacttgttc cgagtcattt ctgctcgcgg
     1441 gcgtgtcttt cgtcgggtcg aacagtccat ctccatcgtc acggtacaaa tacatgccgt
     1501 ccaggtcgcc cagagcgaat ccacttccaa aggaagactc gatccacggc gtcagaggtg
     1561 caaaggcgaa gtcgcccaag tcctcgttga tgccctcatc aattccttca tcaattccct
     1621 catcaacaag accgttcaaa tcgttgtcaa tgccatctgc catctttaga ccggtcacag
     1681 gacagattgg gctgcagaat tccccatcac cgtcgttgtc cagaccgtcc gccatcttga
     1741 acccggtgat cggatcaatt gggcttccat cttcaccgtt tccgttgttg tccaaaccgt
     1801 ccgccatctt cacgccgccg cgataggctt ccagtcccgg catccaacgc agattcgggc
     1861 ttagcggact tccctgttct ccatcgccat catcgtccaa agagatccac tgctcgtaag
     1921 gctgaccgtc ggtcatcgag atgatgccat cggttcctgg gaagacgcac tcgtacacgt
     1981 ccacgccgct ctggggatgg ccaactcgat agtcgtacag tccgttgcca ttgtaatcaa
     2041 agtagatctc atcaccggtg gcggagtagc cccgatccca gcggccatcg ccgtcgaaat
     2101 cttccgtctc ttgttcgaag atgccgttgc catttatatc ttcgtcaaga tggaaatcat
     2161 tgtcgccatc caggtcggtg tactgaatgt cgaaaacgcc gttgccgttt tcatccaccc
     2221 acacgtagta gtccttcaaa tcctcgtcca ccagaggatt catataggtc ggagtgttca
     2281 ggtcttcatc aacaagcgcg aactgtggca ggaaactgtc tcccgggttc atggaaaagc
     2341 cggggttcac gcc
//
LOCUS       DAOQUC010000597         2351 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_56024_length_2351_cov_2.552700, whole genome shotgun sequence.
ACCESSION   DAOQUC010000597 DAOQUC010000000
VERSION     DAOQUC010000597.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2351
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56024_length_2351_cov_2.552700"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..368)
                     /locus_tag="PKH07_10485"
     CDS             complement(<1..368)
                     /locus_tag="PKH07_10485"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNT35410.1"
                     /translation="MASESEVSQLAVNGGEKLRTNPWPGRGHVTEEEKQAVIQLFDQA
                     IQTGHTFGYNGPQEDSLCKEFAEFMGGGYADAVNSGTTAIFVALKALNLKPFTEIVVG
                     AVTDPGGLMPVPLTISVNGL"
     gene            606..1958
                     /locus_tag="PKH07_10490"
     CDS             606..1958
                     /locus_tag="PKH07_10490"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNT35411.1"
                     /translation="MTGKWCEGYPGRCFLILIRVALIFGIVLVGLFGMRKLASLRKPP
                     TRQRLQEPVYQAEAMRVVPEDVVVSLEGFGTVRSRRRVNVSAEVSGRIVEEHPRLQVG
                     EIIPEGEILFRIDPRDYEVALSQASAELDRLKAESARLRTSQQSDSRRLEIARRSLEL
                     MESDFLRVKGLFEQDQVGTKAGVEQAERLFNQQREAVVVLENALALYPTQISENQARQ
                     RSAEAALSKARVNLDRATVKAEFAARIAFEDVEVNQVVSPGKTLVTLVDDSVLEIPVS
                     LDSREAQQWLPFGSDAEGTAWFKLESQPEVRVRWVQDNSERDFLGRLVRVEDYDSTTR
                     TIFVIVEVRAADQAPNATPLVEGMFCSVQIPGKLAQSVYRLPASSVSHEGSVVCAKDS
                     RINIRHVQVILRKQQEVLISGGLESGDVVVTSRLAKIIDGASLELTFADTVPGEGSVE
                     "
     gene            1955..>2351
                     /locus_tag="PKH07_10495"
     CDS             1955..>2351
                     /locus_tag="PKH07_10495"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNT35412.1"
                     /translation="MKSLVALSVHNIPFVNVLMRVILIGGFGASAFLVREMFPAFSAD
                     MIQVTVLYLGADPEDVEEGICRKLEEAIEGIENAKKYSTTASEGVGSAMVEIQEGADI
                     QTVKDEVSEKVNAITTFPTDLILDRLNIGT"
BASE COUNT          528 a          567 c          675 g          581 t
ORIGIN      
        1 ctcaaaccgt tcacggaaat tgtcaagggc acaggcatca gcccacctgg atcggtcacc
       61 gcgccaacga caatttccgt gaacggtttg aggttgagcg ctttgagtgc cacaaagatt
      121 gcggtcgttc cagagttgac ggcatcggca tatccgccgc ccatgaattc cgcaaactct
      181 ttgcacaggc tgtcttcctg cggcccgttg tagccgaagg tatgacctgt ctggatggct
      241 tggtcaaaga gttggattac tgcctgtttc tcttcctctg taacatgacc tcgaccaggc
      301 caggggttgg tcctcaactt ttctccccca ttgacggcaa gctgggagac ttcgctttca
      361 gatgccattt ctaaactcct cgagtggact ttctgcagaa gactgctccc tgtgagtacc
      421 tcggcggtgt ttcgactgtc ggattcttca ggatcatcag cagtacttca ctgtcaattt
      481 ggatactaga atagtcgcac ctgtattgca accctgctga gaaaagaagt atagtacgcc
      541 ttgtgctccg agactttgag gttctctgaa caagtgcata caagtgcata ttggagtttg
      601 tgaagatgac tggaaaatgg tgtgagggat acccgggcag gtgttttttg atcctgattc
      661 gagttgccct gattttcgga attgtcttgg tcggtttgtt tggaatgagg aaattggcat
      721 ctctgcgaaa gcctccgaca cggcaaagac tccaggaacc cgtctatcaa gctgaggcga
      781 tgcgggtggt tcccgaagat gttgttgtga gtttggaggg ttttggcacc gttcgttcgc
      841 ggcgtcgcgt gaatgtcagt gctgaagtct caggacggat tgtcgaggaa catccgcgac
      901 tccaagttgg cgagatcatt cctgagggag agattctgtt tcggattgat ccaagggact
      961 acgaagttgc cctgtctcaa gcgagcgctg aactggatcg gctcaaagcc gagagcgcgc
     1021 gtctcagaac ttcccagcag agtgactcca ggcgtttgga gatagccaga aggagtcttg
     1081 agctgatgga gtcggacttc ctgagagtca aagggctctt cgaacaggat caagttggaa
     1141 ccaaggcagg tgtcgaacag gcagagcgtc ttttcaacca acagcgtgaa gcggtggttg
     1201 ttttggagaa tgctcttgcg ctctacccaa cccagatcag cgagaaccag gcaagacagc
     1261 gaagcgctga ggcggcattg agcaaagcac gtgtgaatct ggatcgcgcc acagtcaagg
     1321 ccgagtttgc cgctcgcatc gcttttgagg acgtcgaggt caatcaggtc gtttccccgg
     1381 gaaaaacgct tgtcactttg gtggatgaca gcgttttgga gattcccgtt tctctggaca
     1441 gccgcgaagc gcagcagtgg cttccctttg gttccgatgc tgagggaacg gcttggttca
     1501 aactcgagtc ccaaccggaa gttcgtgtcc gttgggtgca ggacaactcc gagcgggatt
     1561 ttctcgggcg tttggttcgg gtggaagact atgattcaac aacgcgaaca atctttgtca
     1621 ttgtcgaagt ccgggcggca gatcaggctc cgaacgcgac accattggtg gaaggaatgt
     1681 tctgcagtgt gcagattccc ggcaaacttg cgcagtcagt ctaccggctt cccgcttcgt
     1741 ccgtcagcca cgaaggatct gtcgtatgcg ccaaagacag ccgaatcaac atacggcacg
     1801 tgcaggttat attgagaaag caacaggaag ttctgatctc aggcggactg gagtccggtg
     1861 atgttgttgt cacatctcga ctcgcaaaga taattgatgg ggcgtccttg gaactgactt
     1921 ttgcagacac agtaccgggt gagggtagcg tggaatgaaa agtctcgtcg ctctgtcggt
     1981 tcataacatc ccgtttgtga atgtgctgat gcgcgtcatt ctcattggcg ggtttggtgc
     2041 ttcggcgttt ttggtgcgcg agatgtttcc cgctttctct gccgacatga tccaggtgac
     2101 tgttctctat ctcggtgccg atcctgagga tgtggaagag ggcatctgtc gcaaactcga
     2161 agaagccatc gagggaatcg aaaacgccaa aaagtactcc acgaccgctt ccgaaggtgt
     2221 tggcagtgca atggtggaaa tccaggaagg tgccgatatt caaacggtca aggatgaggt
     2281 cagtgagaag gtaaacgcga tcacaacatt ccccactgac ctcatccttg accgtttgaa
     2341 tatcggcacc t
//
LOCUS       DAOQUC010000598         2350 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_56043_length_2350_cov_3.454902, whole genome shotgun sequence.
ACCESSION   DAOQUC010000598 DAOQUC010000000
VERSION     DAOQUC010000598.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56043_length_2350_cov_3.454902"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1018
                     /locus_tag="PKH07_10500"
     CDS             <1..1018
                     /locus_tag="PKH07_10500"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNT35413.1"
                     /translation="PIIAFLTTIGTYLVWYFGGLGVLRNTLSLGTLMAFIGYLSMFFG
                     PLQMLTHFGDHLNRAFTAAQRLFEIIDADQEVYEDPHARPVRSMEGHFVFKDVHFGYI
                     KDRPVLKGIDVEVQAGEMVGLVGKSGVGKTTMTNLICRFYDVDEGTILLDGVDIRQYR
                     LRDLRQHIGIVPQECYLFDDSILENIRYAKPEATRDEVIQAAIAANAHGFILRLPDGY
                     DSRVGERGARLSGGEKQRVAIARAILHDPKILILDEATSSVDTETEELIQEALKNLVK
                     NRTTFAIAHRLSTLRNANRLLVLEDGKVVEFGTHDELIAREGMYFKLVEMQSKLSSIK
                     VVDG"
     gene            1029..1562
                     /locus_tag="PKH07_10505"
     CDS             1029..1562
                     /locus_tag="PKH07_10505"
                     /inference="COORDINATES: protein motif:HMM:NF020483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1854 domain-containing protein"
                     /protein_id="HNT35414.1"
                     /translation="MDELPKHSRPILIREPDFIFLDGKSLRLFRDDSNKLRLTIDENR
                     SYPDVKVVRAFPLSDPSHYIAFMNHADKVIGMVKDLEALDEQSQALARSSLNRHYFRP
                     VIKRIVEMKEEYGAVYFDVDTEVGRREFVTKGIRDSLQDLGDGLLALADVDGNRFFIK
                     DWTQMDQRSRRFLERII"
     gene            complement(1581..>2350)
                     /locus_tag="PKH07_10510"
     CDS             complement(1581..>2350)
                     /locus_tag="PKH07_10510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35415.1"
                     /translation="AFSTAEITRMKEASERTGKNVVVGLKKMFMPANEKAKQLMDEES
                     FGRTSLVMTQYPQHIPSLDEFDRYLNKEECVWSVVGFLDHLCHPMSLIVFLLGMPKTL
                     YYERAPNAAGLATFTFESGIICSLGLTIGSSINGGMERTCIISDRGRHIVVENNIRLA
                     YHRTPPSEPGTTYGSSPNFFTGTPDQTTAVWEPEFSLGQLYNKGLFLIGYYNEINEFV
                     RSILEKRQPLKGTLDQAWQVTRVFEAFAEGPGKLIQL"
BASE COUNT          557 a          609 c          640 g          544 t
ORIGIN      
        1 gccgatcatc gctttcttga cgacaattgg aacgtacctt gtgtggtact tcggtgggct
       61 aggtgtcctg agaaatacac tgagccttgg aacgctgatg gccttcatcg gctatctgtc
      121 catgttcttt ggcccgctcc aaatgttgac gcatttcgga gatcacctca accgtgcctt
      181 cactgcagcc cagcgtctgt tcgagatcat tgacgccgat caggaagttt acgaggatcc
      241 ccatgcacgg ccagttcggt ctatggaagg acacttcgtt ttcaaggatg tacacttcgg
      301 ttacatcaag gatcgccctg tgttgaaggg cattgacgtc gaggtgcaag cgggagagat
      361 ggttggactg gttggcaaat ccggcgtcgg caagactacg atgaccaacc ttatctgccg
      421 cttctatgat gtggacgagg gaacgattct gttggatggc gtggatattc gtcagtacag
      481 gctccgcgat cttcggcaac acatcggcat cgtgccacag gaatgttatc tgtttgacga
      541 ctccatcctg gaaaacatcc gatacgccaa gcctgaagca acgcgggacg aagtgatcca
      601 ggcagcaatc gctgccaacg cccacggatt cattcttcgt ctccccgatg gctacgattc
      661 acgagtgggg gagcgtggag cgcgcttgtc aggcggagaa aaacagcggg tcgccatcgc
      721 gcgagcgata ctgcacgatc cgaagattct gatcctggac gaggccacgt catcggtgga
      781 cacggaaacc gaggagctga tccaggaagc gctgaagaac cttgtcaaga accgcaccac
      841 cttcgctatc gctcatcggc tgtcaactct gcgcaacgca aatcggctgc ttgttctgga
      901 agacggcaaa gtcgtggagt tcggcacgca tgatgagttg attgccaggg aaggcatgta
      961 tttcaaactg gtcgagatgc agtcgaaact gtcctcgatc aaagtggtag acggctagag
     1021 gaatcatcat ggatgaacta ccgaagcatt ctcgaccgat cctgatccgg gagccagatt
     1081 tcatcttcct cgatgggaag tcactcaggc tgttccgaga tgattccaac aaactgcgcc
     1141 ttacgattga cgagaaccgt tcttaccccg acgttaaggt agtcagagcg ttcccattgt
     1201 ccgatcccag tcactacatt gcattcatga atcatgcaga caaagtcatc ggcatggtga
     1261 aagacttgga ggctttagat gaacaatccc aggctctggc gaggtcttct ctgaaccgtc
     1321 actattttcg acccgtgatc aagcgcatcg tggagatgaa agaggaatac ggcgctgtct
     1381 atttcgatgt agataccgag gtcggacgcc gcgagtttgt caccaagggc attcgcgatt
     1441 cccttcaaga cttgggtgac ggactcctgg cgctggcgga cgtggacgga aaccgctttt
     1501 tcatcaaaga ctggacgcag atggatcaac gctctcgacg ctttctcgaa aggatcatct
     1561 gaccgtcagc gaatcgtgca ctaaagttgg atcagtttgc cgggaccctc ggcaaacgcc
     1621 tcgaacacac gggtcacttg ccatgcctgg tcgagagttc ctttcagcgg ctgccgtttt
     1681 tccagaatcg atcggacaaa ctcgttgatc tcattgtagt agccgatgag gaacaacccc
     1741 ttgttgtaca actgcccgag ggagaactct ggctcccaga ccgcggtggt ctgatcgggt
     1801 gtgccggtaa agaagttcgg cgagctgccg taggtcgtac ctggctcgga aggcggagtt
     1861 cggtggtagg caaggcgaat gttgttctcc accacaatat gccgcccgcg atcggagatg
     1921 atgcaggttc gttccatgcc tccgttaatt gaggaaccga tggtcaaccc cagcgaacag
     1981 ataattcctg attcaaaagt aaacgtggca agtccggctg cgtttggggc acgctcatag
     2041 taaagcgtct tgggcatacc aagcaaaaaa acgatcagcg acatcggatg gcacaggtga
     2101 tccaggaatc caacaactga ccagacacat tcttctttgt tcaaatatcg gtcaaactca
     2161 tccaaactcg gaatgtgttg cggatactgg gtcatcacga gagaagtgcg cccaaaagac
     2221 tcctcatcca tcagctgctt ggccttttca ttggcgggca tgaacatctt cttcagacca
     2281 acgacgacat tctttcctgt tcgttctgag gcttccttca tacgggtgat ttctgcggtg
     2341 gagaaggcag
//
LOCUS       DAOQUC010000599         2350 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_56048_length_2350_cov_3.202614, whole genome shotgun sequence.
ACCESSION   DAOQUC010000599 DAOQUC010000000
VERSION     DAOQUC010000599.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56048_length_2350_cov_3.202614"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2350
                     /locus_tag="PKH07_10515"
     CDS             <1..>2350
                     /locus_tag="PKH07_10515"
                     /inference="COORDINATES: protein motif:HMM:NF012618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35416.1"
                     /translation="SSVYSVAFSPDGTKILTGANLWDATTGALIQTLEGHTGTVTSVA
                     FSREGREIITGGTDGQTLVWELNPPRAIIVAGGGDYSGNSIAQQTDDLGAYAYKTLKR
                     RGYEAQDILYLSAFGPTDPSDPTKPFRDADDDGLNDVDGWSTLGALQAATTGTFAQDA
                     GRLMIILIDHGFITNSGCYFQMNPTQVLPAATLDNWLDDLQAAKPVDVTLVVDACYSG
                     GFVETCKGGVGGHKRIVISSTSSDTVATFLPPPDMTSFMYTFLGSAYMGNSMGESWRA
                     GRQFFEEFPVGGQIPQLDDDSSGTDAADREFFGASWAYGVHSTQDVNDFFPVFDTWMS
                     DGVVAPGTPVTLWTKTLSGQDPLGVVAVVRPPAPPTISGDAVTNLPHVALINSSTDTR
                     LWYATTTDVFTTTGQHVISITARMPYERLSKPVFSRITVSEGTDADTTPIRAILAVGN
                     TFDPDLHSAFAKLGPYAYSVYLDRFQDSGGVHHPEWIEYYINPFIHPESDGYPSSSAV
                     LNAIDAVGSDIGRLYVHLIGDSPGAGLIEMANGDDLSASDLDSKLDAYQVRSVTGTVI
                     LVVDCPYSGSFLSVCKATGAQQRVLMTSGRSTDTALFSKSTQATSFSQKLLGAAYRGN
                     HLRAGFTAGLDFLLTFLQGYIEPQIDDNGDGVSNKFDGQLAQTLFLGRRYAYAGGEAS
                     DLPFVLDVYPEHAGATAGSEVGFTARLMEGVEPARVFAQIVPSDVGSVTEPITSLVEY
                     DFLRDREDAWTWSGSVIVPASEREMSVAVYASYPDGATQEKLT"
BASE COUNT          506 a          623 c          674 g          547 t
ORIGIN      
        1 atcttctgtc tattccgttg ccttttctcc ggacgggaca aagatactga cgggggcaaa
       61 cctgtgggat gcgacaaccg gcgcactgat ccaaactctt gaggggcata cgggcactgt
      121 cacttctgtc gccttctccc gcgaagggag agagatcatc actggtggaa cagacggtca
      181 gactctggtc tgggaactca atcccccacg ggcgatcatc gttgcaggtg gtggcgacta
      241 ttcaggcaac agtattgcac agcagactga tgatcttggg gcgtacgcgt ataagaccct
      301 caagagacgc ggctacgagg cacaggatat cctgtatttg agcgccttcg gaccgaccga
      361 tccttcagat cccacaaaac ccttccgcga tgccgacgac gacggtctga acgatgtgga
      421 tgggtggtca acactgggtg ctttgcaggc tgcaacgaca ggaacgtttg cccaggatgc
      481 cggtcggctc atgatcatcc tgatcgatca tgggttcatc accaacagcg gctgctactt
      541 ccagatgaat ccaacccagg ttcttccagc agccacgctc gacaactggc tcgatgatct
      601 tcaggcggca aagccggtgg atgtgacgct tgttgttgat gcctgctatt caggcggttt
      661 tgtggaaacg tgcaagggtg gcgttggcgg gcacaaacgc attgtcatct cctcgacgtc
      721 ttcagatact gtcgcaacgt ttctgccgcc tccggacatg acttcgttca tgtacacatt
      781 tcttggaagc gcgtatatgg gcaactcgat gggagagtcc tggcgtgccg gcagacagtt
      841 ctttgaagag ttccccgtcg gcggtcagat tcctcagctg gacgacgact cgagtggtac
      901 tgatgccgca gacagagagt tctttggagc atcctgggct tacggagttc actcgacaca
      961 ggatgtgaat gactttttcc cggtgttcga tacctggatg agcgatggcg ttgtggcacc
     1021 ggggacgcca gtcacactgt ggacaaaaac actgtcaggg caagatcccc ttggagttgt
     1081 cgccgtggtt cgtccgcccg cgccaccaac gatttccggt gatgccgtga cgaacctgcc
     1141 tcacgtggcg ctgatcaaca gcagtacgga cacgcgactc tggtatgcaa ccacaaccga
     1201 cgtgttcacc acgacgggac agcacgtcat ctccatcacg gcaagaatgc cctatgaacg
     1261 tctctcgaaa ccggtcttca gccgcatcac agtatcggaa ggaactgatg cggatacaac
     1321 tccaattcga gcgattctcg ccgttggaaa tacatttgat cccgacctgc attcggcgtt
     1381 cgccaagctg ggaccctacg cttacagtgt ctatctcgac cgatttcaag actcgggcgg
     1441 ggttcatcat ccggaatgga ttgagtatta catcaatccg tttattcacc cagaaagcga
     1501 tggctatccg agttcctcag cagtgctgaa cgctattgat gcggtcggct ctgatattgg
     1561 caggctgtat gtccatctga tcggggacag ccccggagca ggtctcatcg agatggcaaa
     1621 tggagacgat ctcagtgcgt cagatctgga ttcaaaactc gacgcctatc aggtacgttc
     1681 agtgacagga accgtcattc tggttgtgga ctgcccgtat tccggttcat ttctgtccgt
     1741 ctgcaaggca acgggagcgc agcagcgggt cttgatgact tctggacgga gtaccgacac
     1801 ggcgcttttt tccaagtcaa cacaggcgac aagtttcagc cagaagttgt tgggggcggc
     1861 atatcgtgga aatcatctgc gagccggttt caccgcaggt ctcgattttt tgttgacctt
     1921 tctgcaggga tacatcgagc cacagataga cgacaacggg gatggcgttt ctaacaaatt
     1981 cgatggacag ctggcacaga cgctgtttct cgggagaaga tatgcctacg ctggtggcga
     2041 ggcctccgac ttgccctttg tgttggatgt ctaccccgaa cacgcgggcg ctactgccgg
     2101 gagtgaagtt ggtttcacgg cgcgtctgat ggaaggcgtg gaaccggcgc gagtgttcgc
     2161 tcagattgtg ccgtcggatg ttggttctgt cactgagccg attacgagcc ttgttgagta
     2221 tgacttcctt cgagatcgtg aagacgcgtg gacatggtct ggatccgtta tcgttccggc
     2281 gagcgaacga gaaatgtcgg ttgcggtgta tgcgtcttac ccggatggcg caacacagga
     2341 gaaactgacc
//
LOCUS       DAOQUC010000600         2350 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_56052_length_2350_cov_3.099782, whole genome shotgun sequence.
ACCESSION   DAOQUC010000600 DAOQUC010000000
VERSION     DAOQUC010000600.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56052_length_2350_cov_3.099782"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1802
                     /locus_tag="PKH07_10520"
     CDS             <1..1802
                     /locus_tag="PKH07_10520"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HNT35417.1"
                     /translation="VERECFGHNPQTPRTLCRWAAVLSVALFVLIATPSAHAYIGPGA
                     GFAFMSSFLVLFATFALAFLMILTWPLRLLYRLIFSRNPYKSATYKKVVVVGLDGLDP
                     KLTKRFMEEGKLPNFSKLAEQGCFVPLRTMSPAMSPVAWSSFQTGVNPGKHNIFDFLT
                     RDPKTYFPVLSSCVTGSGGKAELRLLRKSTPFWVLLGNKGVFGSILRVPITFPPEKFR
                     GVSLSAMCVPDIRGSQGTFTFYSSQNGSGVTHTGGVQIPVRVSDNRIETYISGPPAPG
                     SEGGELRIPITIKVLPDANAAELTVGDEVIHLRQGIYSNWVTLEFKIGRWSSVKGICM
                     FMLRQVSPQFELYVTPINLDPESPAMPVSHPVAFSVYLSKMQGKYATLGLAEDTWALN
                     ERVIDEDAFLKQAYQYHQEREEMFFNALSKTRKGVCACVFDATDRIQHTFWRYLEPDH
                     PANRNKEQDRHKNAIEDLYVRMDDLVGRTMAKLDDKTLFFVMSDHGFCSFRRGVNLNA
                     WLHQNGYLALKDGLKESGDYFQGVDWSKTKAFTLGLTGIFLNIKGREAQGTVEPGQEA
                     EALKKEIIEKLKDLKDEQTVRLVFVRHLTARRT"
     gene            1799..2092
                     /locus_tag="PKH07_10525"
     CDS             1799..2092
                     /locus_tag="PKH07_10525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35418.1"
                     /translation="MTGPYVVNAPDLVIGYNRGYRASWEAAVARITSEVFDDNTKSWS
                     GDHCIDWRQIPGVLFCNRKVDKSDPGIMDMAPTILQNFGVPVPAYMDGRPLFE"
     gene            2159..>2350
                     /locus_tag="PKH07_10530"
     CDS             2159..>2350
                     /locus_tag="PKH07_10530"
                     /inference="COORDINATES: protein motif:HMM:TIGR01409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HNT35419.1"
                     /translation="MSINRRDFIKGSVAAGLGVGIGSLVKSSDQEAFAAASGAKHRVL
                     ILGIDGMDPELLLRYVNKGI"
BASE COUNT          546 a          619 c          631 g          554 t
ORIGIN      
        1 cagttgaaag agaatgcttc ggtcataatc cgcagacacc gcggacattg tgccgttggg
       61 ctgcggtgct ctcggtggcg ttgtttgtat tgatcgcaac cccctccgcc catgcctata
      121 ttgggcctgg tgccgggttc gcgttcatgt cttcgtttct cgttctcttt gccacgtttg
      181 cgttggcttt tctcatgatc ctgacttggc cacttcggct gttgtaccgg ttgattttta
      241 gccgcaaccc gtacaaatcg gcaacgtaca agaaagtggt cgttgtggga ctcgacggat
      301 tggatcccaa actgaccaag agattcatgg aagagggtaa gttgccgaac ttctccaaac
      361 tcgccgagca aggctgcttc gtgccgttga gaacgatgtc gccagcgatg tctcccgtgg
      421 cgtggtcctc cttccaaacg ggcgtcaatc ccggtaagca caacatcttc gactttctca
      481 cacgcgatcc caagacgtat tttcccgtgt tgtcctcttg cgtgaccggt tccggcggaa
      541 aagccgagtt aagactgctc cgcaagagca ctcccttctg ggtgttgctg ggcaacaaag
      601 gtgtcttcgg ttccattctt cgggttccga tcacattccc gccagagaag tttcgcggag
      661 tctccctctc ggcaatgtgt gttcccgaca ttcgaggctc tcaagggaca ttcacgttct
      721 acagcagcca gaacggatcg ggcgtcacac acaccggcgg tgttcagatt cccgtcagag
      781 tttctgacaa ccgaattgaa acgtacattt caggccctcc tgccccaggg tctgaaggtg
      841 gtgaactgcg cattccgatc actatcaagg tcttgcccga tgccaatgcg gcagaactga
      901 ctgtgggcga tgaagtcatt catcttcggc agggcatcta ttccaattgg gtgactctgg
      961 aattcaagat cggccgctgg tcttcggtca agggcatctg catgttcatg cttcgtcagg
     1021 tcagcccgca gtttgagctc tatgtcaccc caatcaacct tgatccggaa agccctgcaa
     1081 tgccagtctc ccatccagtg gcgttctcgg tctatctttc aaaaatgcag ggcaaatacg
     1141 ccacgctcgg tctggctgag gacacctggg cactcaacga acgggttatt gatgaagatg
     1201 cgtttctgaa gcaagcctat cagtatcacc aggaacgcga ggagatgttc ttcaacgccc
     1261 tctccaaaac tcggaaaggg gtgtgtgcct gcgtgtttga tgcgaccgac cgcatccagc
     1321 acacgttttg gcgctatctg gaacccgatc atcctgccaa ccgaaacaag gaacaagacc
     1381 gacacaagaa cgccattgag gatctctatg ttcgcatgga cgatctggtc gggagaacca
     1441 tggcgaaact tgatgacaag acgcttttct ttgtcatgtc cgaccacgga ttctgttcat
     1501 tccgacgtgg agtgaatctc aatgcctggc tgcatcagaa tggatacctc gccctgaaag
     1561 atggactcaa ggaatcagga gactatttcc aaggcgtgga ctggtcaaaa acaaaggctt
     1621 tcactctggg tctcacgggc attttcctga acatcaaagg tcgtgaagcc cagggaactg
     1681 tcgaacccgg ccaagaagcg gaagccctca agaaagagat catcgaaaag ctgaaagacc
     1741 tcaaagatga gcagaccgtg agattggtat tcgtgaggca tttgacagca agacgcacat
     1801 gaccggccct tacgtggtca acgccccaga cctggtgatc ggatacaaca gaggctatcg
     1861 ggcatcctgg gaagcggcag ttgcccgcat cacatcagaa gtttttgacg acaacacaaa
     1921 gtcctggagc ggcgaccact gcattgactg gagacagatt ccaggtgtct tgttctgcaa
     1981 cagaaaggtg gacaagtctg atcccgggat tatggatatg gcaccaacga tactgcagaa
     2041 ttttggagtg cccgtgcctg cttatatgga cggaagacct ctttttgagt aatcttccag
     2101 cgaatgggcg cgttgtggag atgacagcgg gggagcgcct ggagaggatg gtacagatat
     2161 gagtatcaat agaagagatt tcatcaaagg gtctgttgca gcaggccttg gggtcgggat
     2221 cggctccctt gtgaaatcgt ccgatcagga agcctttgcc gcggcatcag gggccaaaca
     2281 tcgagttcta atcctcggca ttgacggcat ggatccggaa ctacttctgc gctatgtcaa
     2341 caagggcatc
//
LOCUS       DAOQUC010000601         2350 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_56054_length_2350_cov_2.846187, whole genome shotgun sequence.
ACCESSION   DAOQUC010000601 DAOQUC010000000
VERSION     DAOQUC010000601.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56054_length_2350_cov_2.846187"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1322
                     /locus_tag="PKH07_10535"
     CDS             <1..1322
                     /locus_tag="PKH07_10535"
                     /inference="COORDINATES: protein motif:HMM:NF012936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HNT35420.1"
                     /translation="HRGGALRSTLNEHSVTMHATWNEWEICPIASSICTKESCNGAFE
                     ASAMTETLSKGTRPFALARPCEEGVFLARDPLGVRPLYYGEADGVLAFASEVKALLTV
                     TDDVNEFPPGAWYTPQDGIQEFARLRVGSLHFGNVATIASELRDILQDAVVRRIHSDE
                     LGSWLSGGLDSSVIAALARPHVKTLHTFVSGVQGAPDIEFSCQMAEYLRTRHHVLTVR
                     LDDLLSALPDVIYYLESFDALLVRSSVTNYLTAKIVSDHVGSVFSGECGDELYAGYDY
                     IKGLPTEKISEELDDIIKRLHNTALQRVDRCAHAHGVVPLIPFADMNVVRHALRIPSK
                     YKIHWDGGKPIEKWILRKAFEGLMPDNVLWRPKAKFWQGTGVEDLLSRYASSTVSDSD
                     FARERILPNGWVLDSKEELMYYRVFKEHFGTLNQLDWMGRTKRIPVV"
     gene            1315..1644
                     /locus_tag="PKH07_10540"
     CDS             1315..1644
                     /locus_tag="PKH07_10540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35421.1"
                     /translation="MYNPGDGETRSSHAAIHQQSDSCLGVDSSKHLAEIGGGSFLIGE
                     FEDSNGVPYVLIVNKNIHISSAFHVKFKEPGTVMMTSPYTDQTYGFGGENGWLSRGSG
                     ILLSLGK"
     gene            complement(1633..>2350)
                     /locus_tag="PKH07_10545"
     CDS             complement(1633..>2350)
                     /locus_tag="PKH07_10545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35422.1"
                     /translation="VVQGLGCTSMLVTFLLMGVFSFPFLTFVAVMLVLNIALWFVRKE
                     MPQRMDYNLFLRASRHGLGTTRTSRMAVSLSGADLWVFARFLGKHFLILDCSVDESGC
                     RLYCPAVASDFLRAFLMPIARWNGSSTISVSRNGECRVHLSRRDARQIAAVTKNQCPA
                     VEEIEKSVRLAIAKALGAFMAGDQSKALSVLQSESDECIFVRPPHQLRSQKVVYLVAI
                     VAGIFLLLALIMLRGIRLFP"
BASE COUNT          573 a          577 c          645 g          555 t
ORIGIN      
        1 gtcacagggg cggggcactc agaagcacac tgaatgagca tagtgttacc atgcatgcta
       61 cctggaatga gtgggagata tgcccaattg cctcatctat ctgcacgaaa gagtcctgca
      121 acggtgcatt cgaggcttcg gctatgacgg agacgctctc caaaggaacg aggccctttg
      181 ctctcgctcg tccttgtgaa gagggtgtat ttcttgcgcg tgatccgctg ggtgtcagac
      241 ctctttacta cggtgaggcg gatggggttc ttgcttttgc ctcggaggtg aaagcgctgc
      301 taactgttac ggacgatgtg aacgagtttc ctccaggcgc ttggtatact ccgcaggatg
      361 gtattcagga gtttgcccgc cttcgcgtcg gcagtctgca cttcggtaac gtcgccacca
      421 ttgcgtctga gttgcgtgat atactgcagg atgctgtcgt tcggcgcatt cattctgatg
      481 agttgggttc atggctttct ggcggtctgg attcatcagt gattgcagcg ctggcgagac
      541 cccacgtgaa gacgttgcat acctttgtaa gcggagtgca aggtgcgcca gatatcgaat
      601 tcagctgcca gatggcggag tacttgagaa cgagacatca tgtgctgacc gtcaggctgg
      661 atgatctact ttcagcacta ccggacgtta tctactatct tgaatctttt gatgcgctct
      721 tggtgcgctc ttcggtgacc aactatctga cggcgaaaat cgtttcggat cacgtgggat
      781 cagtattctc gggggagtgc ggtgatgaac tgtatgcagg ttacgattac atcaagggac
      841 ttccaacaga gaagatatct gaggaactcg acgacatcat caagcgcttg cacaatacag
      901 cacttcaaag agtagatcgt tgtgctcatg ctcacggtgt agttccgctg attccttttg
      961 cggatatgaa cgtagtgaga catgcacttc gcattcccag caagtacaag atacattggg
     1021 acggagggaa accaatcgag aagtggattc tgcgcaaagc ctttgagggc ttgatgccgg
     1081 acaatgttct ctggcgtccc aaagcgaagt tctggcaggg aactggtgtc gaggatctgc
     1141 tttcccggta cgccagttca actgtgagcg attccgactt cgcgcgggaa cgaatcctcc
     1201 ctaatggttg ggtgctcgac agcaaggaag agctgatgta ttaccgggtt ttcaaggagc
     1261 attttggcac tctcaatcag ctggattgga tgggtcgaac aaagagaata cctgttgtat
     1321 aatcccggcg atggcgagac tcgcagttcg catgctgcga ttcatcaaca atcagactca
     1381 tgccttggcg tggatagcag caagcacctt gccgagatcg gtggcggcag ttttctcatt
     1441 ggggaatttg aggactcaaa cggcgttccc tatgtgctga tcgtcaacaa gaacatccac
     1501 atctcatcgg cgttccatgt gaagttcaag gaacccggca cggtgatgat gaccagccca
     1561 tacacagatc agacctacgg atttggtggt gagaacggct ggctgtcacg cggaagtgga
     1621 atcctgctca gtctagggaa ataggcgaat gcctctcagc attatcaagg ccaacagcag
     1681 aaagatgccg gcaacaatcg ccacaaggta gacgactttc tgacttcgga gctgatgggg
     1741 tggtcgcaca aagatgcatt catcgctttc tgattgaagt acgctcagag ctttactttg
     1801 gtctcctgcc ataaaagccc cgagtgcctt ggcaattgcc aagcgcacag atttctcgat
     1861 ttcctccacg gcagggcact gattcttagt aacggcggca atttgacgtg catcgcgtcg
     1921 ggacaagtga accctgcatt ctccattgcg gctgacggaa atcgttgaag aaccgttcca
     1981 gcgggcgatg ggcatcaaga aagccctcaa aaagtcggac gcaacagcag gacaataaag
     2041 acggcatccg ctctcatcca ctgagcagtc caagataaga aagtgttttc cgaggaatct
     2101 cgcaaacacc caaagatcgg cgcctgacag actcacagcc attctgctgg ttcgggttgt
     2161 tcccaagccg tgtcgagatg ctctcaggaa aagattgtag tccatccttt gtggcatttc
     2221 cttgcgaacg aaccagagag ctatgttcaa cacaagcatc acagcgacaa aggttaagaa
     2281 ggggaaactg aagacaccca tcagcagaaa ggtgacaagc atggacgtgc acccaagccc
     2341 ctgaaccact
//
LOCUS       DAOQUC010000602         2346 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_56204_length_2346_cov_2.791357, whole genome shotgun sequence.
ACCESSION   DAOQUC010000602 DAOQUC010000000
VERSION     DAOQUC010000602.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2346
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56204_length_2346_cov_2.791357"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..558)
                     /locus_tag="PKH07_10550"
     CDS             complement(<1..558)
                     /locus_tag="PKH07_10550"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HNT35423.1"
                     /translation="MNPENTNVARKRDAKGAWSDWLTGLDVLLVFVLALGTSFLVYEA
                     KQKIRYETYGYESPVLVWCAPLILVSLFGLSMRIGRNLLYFIAGIVVGIAVITFGDVL
                     SLSTHLATFVALGLMLALFNGWAWRSWLALPRVRALRREEKEYMKRMSGMTLIEILIV
                     IALIGVMSAGLIRMEARTRITGQQLE"
     gene            complement(548..1714)
                     /locus_tag="PKH07_10555"
     CDS             complement(548..1714)
                     /locus_tag="PKH07_10555"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HNT35424.1"
                     /translation="METTQAIVMEGEPNASDAEAYVTWLFQKAVQQRASDVHVQPHAD
                     GTRVLYRIDGVLREATRFESTNHERVISRLKVLACLPVYQHELPQEGRIEQTPGLCPA
                     RLAVFPTIHGEKAVVRLFHRLGERPLQLDELGFIPQIVKGLRQMTEGKSGCILLTGAS
                     GSGKTTTIYSLLRDIYCRRGDSVNVVTLEDPVEMNLEFAVQSEIKPAVGYTFATGLRS
                     ILRQDPEVLMLGEIRDRETAQMVVEAGLTGHLVISTIHSGNCVEVFTRLLEMGIEPYL
                     VASSILGVLSQRLVRRKCPACLGKAVSPDGLPCEECQSTGFRGRTAVGEMLVVSDPFR
                     KAVLRRLPTSELTEVSRENRIGSLLIQPDLSTSGTMEPSENLTPTLQQGVSHES"
     gene            complement(1776..>2346)
                     /locus_tag="PKH07_10560"
     CDS             complement(1776..>2346)
                     /locus_tag="PKH07_10560"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNT35425.1"
                     /translation="DVLIQCCAFLLTALMFLMFGFLVFLGWGRGNRFLDRLSLNATFW
                     GTILRQSHASVVGKVLSGLLEAGVSLSESLILCAEMTPNACVRDSLLLAHRKVNQGES
                     LGQALRIYPVFSEWFNWSVESLEKMSALPQGLSVLAEQLEYRTRLAITLFHRVLSPIL
                     VVTVWLATIAILYAPFFRISRMLGLFMIN"
BASE COUNT          611 a          679 c          568 g          488 t
ORIGIN      
        1 ttccagctgt tgtcccgtga tgcgagtgcg tgcctccatg cgaatgagtc cagcagacat
       61 gacccctatc agggcaatga caatcaagat ctcaatgagt gtcatgccgg acatacgttt
      121 catatactcc ttctcctctc ttctcaaggc tctcactcgt ggcaacgcca accaggagcg
      181 ccaagcccaa ccgttgaaca gagcgagcat cagaccgaga gccacgaacg tcgccagatg
      241 ggtagacaga ctcagaacat ctccaaaggt aatcacggca attccaacaa cgatgccagc
      301 aatgaagtac aggagattgc gccctatcct catggacaga ccgaacagag acaccaggat
      361 caaaggtgca caccacacaa gaacaggtga ttcataccca taggtctcgt agcgtatctt
      421 ctgttttgct tcgtagacca gaaaactcgt tccaagcgcc aagacaaaga ccaggagaac
      481 gtccaggcca gtcagccaat ctgaccatgc gcctttcgca tctcgctttc gtgcgacatt
      541 ggtattttca ggattcatga gagactcctt gctgtagagt aggcgtgaga ttctccgaag
      601 gctccatggt tccggaggtg ctcagatctg gctgtatcag cagacttccg attcggttct
      661 cacgggagac ttcggtcagc tccgaagtgg gaaggcgacg caagacggct ttccgaaatg
      721 gatcgctgac aaccaacatt tccccgacgg ctgtacgacc tctgaaaccg gtggattggc
      781 actcctcaca cggcagacca tccggtgaga cagccttgcc cagacaagcc ggacacttcc
      841 ttctgacaag ccgttgactg agaacaccca ggatcgagga agcaacgaga taaggctcaa
      901 ttcccatctc caagagtcga gtgaacactt cgacacaatt gccactgtgg atggtactga
      961 tcaccagatg ccctgtcagc cctgcttcca caaccatctg tgccgtctcc ctgtcccgaa
     1021 tctcaccgag catcagcacc tcgggatcct gtcggagaat ggatcgcaaa ccggtggcaa
     1081 aagtgtatcc caccgcaggt ttgatctccg actgaacggc gaactccagg ttcatctcaa
     1141 caggatcttc gagtgtgaca acgttgaccg aatctcctcg acgacagtag atatcccgaa
     1201 gcagagagta gatggttgtc gtcttgcctg aaccgctggc cccagtcagc agaatgcagc
     1261 ccgatttccc ctctgtcatc tgtcgaagac ctttcacgat ctgagggata aaccctagtt
     1321 catccaactg aaggggtcgc tctccaaggc ggtgaaatag acggacgacg gctttctccc
     1381 cgtggatcgt cggaaacact gccagacgcg caggacacaa tccaggcgtt tgctcgatgc
     1441 gcccttcctg tggaagctcg tgctgataga cgggaagaca ggcaagcacc ttgagccgag
     1501 agatcacccg ctcgtggttg gtggactcaa agcgagtcgc ttcccgcaat acaccgtcaa
     1561 tccgatacag cacgcgagtc ccatcagcat gtggctgaac atgaacatct gaagccctct
     1621 gctgaacggc tttctggaat agccaggtca cgtaggcttc ggcatcggaa gcgtttggct
     1681 cgccttccat aacgatcgct tgggttgttt ccatcctatt cttccttttc gtcttctcgt
     1741 tcatggtatt cgtcctgaac tcattcccac cttcatcagt taatcatgaa aagccccagc
     1801 attcgagata tcctgaagaa aggagcgtaa aggatcgcaa tcgtcgccaa ccagacagtg
     1861 accaccagaa tcggagaaag aactcgatga aacagggtaa tagcaagtct ggttcggtat
     1921 tccagttgct ctgccagcac agaaagtccc tgcggcagtg cactcatctt ttccaaagac
     1981 tcgaccgacc agttgaacca ctcagagaaa acaggataga ttctcagtgc ttgccccaga
     2041 gactcacctt ggttcacttt gcggtgcgcc agaagaagac tgtctcgcac gcaggcgttt
     2101 ggtgtcatct ccgcacaaag aatgaggctt tcagacaaag acacaccggc ttcaagcaga
     2161 ccactcagca cctttccaac aacagaagca tgtgactgcc gaaggatcgt accccaaaac
     2221 gtcgcgttca gactgaggcg gtcgagaaac cggttccctc gtccccaccc gagaaacact
     2281 aggaatccga acatgagaaa catcagcgct gtgagaagaa acgcgcaaca ctggatcaag
     2341 acatca
//
LOCUS       DAOQUC010000603         2344 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_56275_length_2344_cov_3.131935, whole genome shotgun sequence.
ACCESSION   DAOQUC010000603 DAOQUC010000000
VERSION     DAOQUC010000603.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2344
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56275_length_2344_cov_3.131935"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            783..1328
                     /locus_tag="PKH07_10565"
     CDS             783..1328
                     /locus_tag="PKH07_10565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35426.1"
                     /translation="MKRKLMFVMVGVTVLFVFVGLRGLRGDEGEVSPDEEGSCFVYPE
                     QWPENPLSGSFGEYPEQTPVSCEDNDDSDGILAFPEQGEDPGDSNDISPVSNLGPIDG
                     AYDEEALDGDGFRELGMQVTCWITEDECTGDYRIPVGHANGSYKVLYGRVANPTAYYQ
                     NNRCGYGVEVGRVSDLEDMIV"
     gene            1347..>2344
                     /locus_tag="PKH07_10570"
     CDS             1347..>2344
                     /locus_tag="PKH07_10570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35427.1"
                     /translation="MDERTDDGGDVKITIDLFEHSSGSCGGRVTRNFNADTATCQVGN
                     NDTDKGWYAAWSDGKADCAGGYSDPGDCHLPDIFVSYPGSPGNYAYTYEIDVDVCDFD
                     GTNCTNDSESGCFIVEWYCPVKVGIENGSKASSIGTTWDPPASTLARACAVSPDPCTD
                     DYRLMLGSGYGKYSSRIRNAEAVANFTANTCGLQQDVGEIPSDEDLIIYVDTSYAIDT
                     TASDGGIVKVKIDVDEWNTTHGVEPCTGDKNRTFPSDTAECDVDGTASNRSWQTPWSD
                     SKGDCSGGYGDPGDCHNPDIGISFPSGSGTGTYCYEYYIEISVCDSDGTNCTTDKET"
BASE COUNT          537 a          533 c          694 g          580 t
ORIGIN      
        1 ttcgagtgct ctgctggttg ccagtattgc tgctctgttt ttccgggtgc tcacatcacg
       61 gctcatctgc tgctcccggt gtatcagccg acgtggcgga ccaaagttcc tcaaatgact
      121 cacgagattg cgggaccgat cacgacggtc attcggatct cgcgccaacc atggtgatac
      181 cggacagcct agactcaagt atgcacgatg ggaaagagga ggatcctgac atcctgcgta
      241 acggatccga tgttttggac gcgagcgacc tgctgcccga aggttgctgc tacaaggata
      301 gcgattgcga aagtggaatg ctttgcctcg cacctggaga ggacggtctg ggtcattgtg
      361 cacaattgcc gttggacgaa cactgttact tggatgcaca atgtcccacc ctctttggtt
      421 gcttcgaaca aaccatatgc tcttgcggga cggcgtgcga catcatcgaa ggctcatgca
      481 agccaatcca tggctcctgc tgcgacgatg acgcagactg tccgccgggc ggcgtgtgtg
      541 tggcaccaga ttcaccaggc aagaatgctt gtctgcccga gccccgaccg ggcgaatgtt
      601 gggaggatag ccactgtgga acgaaagaaa cttgcattgg tgctctgtgg tgtggctgcg
      661 cattctattt ctgtgatcct gcggtgtttg gcgtgtgcct tccggaacaa taggatggca
      721 gacgatattg ctattgactt caggatcagg ctgctgagtt tattttcacg gggagggtgc
      781 cgatgaagag aaagctaatg tttgtgatgg ttggcgtgac cgtacttttt gtttttgttg
      841 gtctgcgagg tcttcggggc gacgagggcg aagtgtctcc agatgaggag gggagctgtt
      901 tcgtataccc ggaacaatgg ccggagaacc ccttgagcgg cagtttcgga gagtatccgg
      961 aacaaacccc cgtctcgtgc gaagacaatg acgattccga tggcatcttg gcattcccgg
     1021 agcagggaga ggacccaggt gattccaatg atatttcacc tgtttcgaac ttaggtccta
     1081 ttgacggtgc ttatgacgag gaagctctgg atggagacgg atttcgggaa cttggaatgc
     1141 aggttacatg ttggattacc gaggatgaat gtactggaga ctatcgaatt cccgttggcc
     1201 atgcgaacgg ctcctataag gtattatacg gacgcgttgc gaaccctacg gcatactatc
     1261 agaataacag gtgtggttac ggggttgagg ttggacgcgt ttcggacctc gaggatatga
     1321 tcgtctaagt tccccatgct ctcgccttgg acgaacgtac agatgatggt ggggacgtca
     1381 agatcaccat tgacctattt gagcacagtt ccggttcttg cggagggcgg gtgacccgga
     1441 atttcaacgc tgacacagct acctgccaag tgggcaacaa cgacactgac aaaggctggt
     1501 atgctgcatg gtctgacggc aaggctgact gtgctggtgg atacagtgat ccgggtgact
     1561 gccatttgcc cgacatattt gtgagttatc ctggttcacc tggcaactat gcctatacct
     1621 atgagataga tgttgatgtc tgtgattttg atggtaccaa ttgtacaaat gactctgagt
     1681 cgggttgctt cattgtggag tggtactgcc ccgtaaaagt tggcattgag aatggaagca
     1741 aggcgtcttc cataggtacg acctgggatc cacctgcaag tactctagct cgggcttgtg
     1801 cagtatcgcc ggacccttgc accgacgact atagactcat gttgggttct ggatatggta
     1861 agtattcatc aagaatcaga aatgctgaag ctgttgccaa ctttacggca aatacatgcg
     1921 ggttgcagca agacgttgga gagatcccaa gcgatgaaga cttgattatt tatgttgaca
     1981 cgtcttatgc tattgacacg acagcgagcg acggcggcat agtgaaggtt aagatcgatg
     2041 tggacgaatg gaataccacg cacggggttg agccttgtac gggagacaag aatcgcacct
     2101 ttccgtccga tactgccgaa tgtgacgtag atggtacagc ttctaatagg tcgtggcaga
     2161 ccccgtggtc tgactcgaag ggggactgtt ctggtgggta tggcgaccca ggcgactgtc
     2221 acaatccaga catcgggatt agtttcccgt ctgggagtgg aacgggcacg tactgttacg
     2281 aatattacat tgagattagc gtgtgcgatt cggatggaac gaactgtaca acagacaagg
     2341 agac
//
LOCUS       DAOQUC010000604         2344 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_56277_length_2344_cov_3.084316, whole genome shotgun sequence.
ACCESSION   DAOQUC010000604 DAOQUC010000000
VERSION     DAOQUC010000604.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2344
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56277_length_2344_cov_3.084316"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1376)
                     /locus_tag="PKH07_10575"
     CDS             complement(<1..1376)
                     /locus_tag="PKH07_10575"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNT35428.1"
                     /translation="MSQEKPNILLITADQQRFDTAGSAGPSFMRTPHFDHLCNEGVCF
                     TSAYADCPLCVPSRVTIMTGQSVFTHGMYGNGSSSKVLVREQTLPSYLRQLGYQTAAI
                     GKMHFTPQRARHGFDEMILPEDYYREMIRSGCLSQPMHHGLGQNELYPAMATVPESQT
                     LTSWTAEQCVQYIRERRDPTVPFFLWCSFSKPHPPLDPPEPYYSMYRNSPIPEPVFGD
                     WSEDDRCPETFKRFRQKQGYDLLPREIIREARAAYYGLITQIDYNMGRVFAALQDLGL
                     FADTLVLYTSDHGEFLGDHHTGSKIFFHEPSAHIPFVLRMPHCWENRCEGTKSRNLVS
                     LADVLPTLLAAAGGSAPAGVDGQNLVALSRGEVAPAPFLMSRGEPGCQQVGITDGRWK
                     YIYYTEGAQEQLFDLETDPKELVDLAGNPGFRDEKKRLGREMIQALEGKQTDLIEQGE
                     LVKKPMGT"
     gene            1502..>2344
                     /locus_tag="PKH07_10580"
     CDS             1502..>2344
                     /locus_tag="PKH07_10580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35429.1"
                     /translation="MNRFSTWKGIPSAIVMSLLYIAQRLWVSLSGRCLLDSDEGIVLI
                     AANDILHGQLPVFFYGQDYLGSFQSFAAVPFVTVLGVTPLALRLTAIFEGVALLWIWR
                     WILRKWGSPEIWVLFALLLVFAPEFATVWTLRSRGGIETLLLGSLWIASLTTILSTDR
                     VSSFHWFRLGLLTGIAWWTSQLTVFFIAAGLPLLLLTRSGRSRMTQLDTAEHCETGLP
                     DWALGILYLLSIGLLAARGTVPYRSPMSAVLLKHRSTLIGLCSVTLFVYLIWATVRRR
                     KPWPL"
BASE COUNT          495 a          636 c          621 g          592 t
ORIGIN      
        1 tcggtgccca ttggtttctt gaccagctcc ccctgttcaa tcagatcggt ctgctttccc
       61 tccagcgctt ggatcatctc ccgtccgagt ctcttcttct catctcggaa tcctggattc
      121 ccagccagat cgacgagttc cttcggatcg gtctccagat caaagagttg ttcctgtgcg
      181 ccttcggtgt agtagatgta cttccatctt ccatccgtga tgccgacttg ctgacagccg
      241 ggctcccctc tggacatcaa gaacggcgca ggagcaactt ctcctcttga aagtgcaaca
      301 aggttctgac cgtccacgcc cgcaggagca ctgccacccg ccgctgcgag caatgttgga
      361 aggacatctg ccagtgagac aagattgcgg gacttcgttc cttcacagcg attttcccaa
      421 cagtgaggca ttcgcagaac aaaaggaatg tgcgctgacg gctcatggaa gaatatcttt
      481 gagccggtgt gatggtcacc gagaaactca ccgtggtcgc tggtgtaaag cacaagcgta
      541 tctgcaaaga gcccaagatc ctgaagcgca gcaaaaactc tacccatgtt gtagtcaatt
      601 tgagtgatca gcccgtaata ggctgcccgt gcctcacgga tgatctctcg cggcagcaaa
      661 tcatagccct gcttctggcg aaaacgcttg aaagtttcgg gacaacgatc atcctcagac
      721 cagtctccaa acacaggctc gggaatcgga gagtttcggt acatcgaata gtacggttct
      781 ggcggatcca gaggcggatg cggttttgag aaagaacacc acaggaagaa cggcacagtt
      841 ggatctcgac gttcccgaat atactgaaca cattgctcgg cagtccacga tgttagcgtc
      901 tgactctcgg gaaccgttgc catggccgga tagagttcgt tttgacccag accgtggtgc
      961 atcggctgag acaagcaacc cgaccggatc atttctcgat agtagtcttc gggcaggatc
     1021 atctcatcaa aaccatgtcg tgcgcgctgt ggcgtaaagt gcatcttccc gatggctgcg
     1081 gtttgatatc ccaattgcct caaatacgaa ggcagcgttt gctcgcgtac caggactttg
     1141 gaactcgaac cattgccata catgccatgc gtgaataccg actgaccggt catgattgtg
     1201 acccgtgagg gaacacacaa agggcagtcc gcgtaggctg aagtgaaaca gacgccttca
     1261 ttgcagagat gatcgaaatg cggtgttctc atgaacgaag gcccggctga tccggcagtg
     1321 tcgaagcgct gctggtcggc tgtgataagc aagatgttcg gcttctcctg agacatacaa
     1381 gatcctccct gttgttgccg tgcagtacgc tggggacact cggtctctca aatgcccccg
     1441 tcacggatca agatagcaca caaacgactc aacaatgtag aagatatgat atgatattcc
     1501 gatgaatcgg ttctcaacat ggaagggtat cccaagcgcg attgtgatgt ctctgctcta
     1561 tatcgcgcaa aggctttggg tgagtctctc gggaagatgt ctgctggact ccgacgaagg
     1621 cattgtcctt atcgcggcca acgacatctt gcacggtcaa ctacccgttt tcttctatgg
     1681 acaggactac ctgggcagtt ttcagagttt tgccgcggtt ccttttgtca cagttctcgg
     1741 agtgactcca ctggctttgc gactgactgc cattttcgaa ggcgtcgcat tgctttggat
     1801 ctggcgatgg attctccgca agtgggggtc cccggaaatc tgggtgttgt ttgcgctcct
     1861 tttggttttc gccccggagt tcgccaccgt ctggacgctt cgatcgcggg gtggcattga
     1921 gacgctcttg cttggttccc tttggattgc ctctctgaca accattctca gcaccgacag
     1981 agtttccagt ttccattggt ttcgtctggg cttgttgaca ggaattgcct ggtggacgag
     2041 ccaactgacg gtgttcttca tcgctgcagg tctgcctctt cttcttctga cgcgatctgg
     2101 acggagtcgg atgactcagt tagatacggc tgagcattgc gagacaggtc tcccggactg
     2161 ggctctgggg atcctctacc ttctcagtat cggtctgctc gctgcccgtg gcactgtgcc
     2221 ctaccgatcc ccgatgagtg cagtgctttt gaagcaccgc agtacattga ttggactgtg
     2281 ttctgtcact ctcttcgtct accttatctg ggcgactgtg cgacgcagaa agccttggcc
     2341 actt
//
LOCUS       DAOQUC010000605         2340 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_56417_length_2340_cov_3.529540, whole genome shotgun sequence.
ACCESSION   DAOQUC010000605 DAOQUC010000000
VERSION     DAOQUC010000605.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2340
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56417_length_2340_cov_3.529540"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(94..849)
                     /locus_tag="PKH07_10585"
     CDS             complement(94..849)
                     /locus_tag="PKH07_10585"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNT35430.1"
                     /translation="MIIRSLVTMSTVQAGVADTLRRANTTTEPIGKLIVLLLVVFSVV
                     LWAVAGLKILQFWRSMWNNRRFLAEFNTISNDFQLAHERAQEHRGSPLASIFSEVYRE
                     LRALARPDTGKLAMTPGMVDLVQRAADRQIALLVSQMDRYISILGTIAHISPLLGLFG
                     TVWGVLVAFTDMGVAGTTDLTVVAPGIAGALITTVAGLAAAIPAAVCHNYLNRRIERQ
                     LEEMEDFASRLTSYLDRSILRRAGRQQQEQQTP"
     gene            complement(876..1670)
                     /locus_tag="PKH07_10590"
     CDS             complement(876..1670)
                     /locus_tag="PKH07_10590"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNT35431.1"
                     /translation="MRPTMKKALVIVLMCSSFGCATGQLRRIEERIGVLETRLEIFNE
                     NYDRSSDKSQQSLQDLYSTLKKQNELVQNNQNRMNSAIEDLSAEVLKMRREVDDMKSR
                     VARVETRIDSAEGVLKTELKGAQDSLQKEAKDYQDGMGKQMTDFQAQTRKSLGDLEAK
                     LSKDITSSEERNKGSINALRNEVSTSFRDLEKIIRGLSSDNTVAPTSSGVSTDPSGTI
                     HKVAPGETLSAIAQKYGVSTNEVIRVNNITDAAKIQVGQEIVIPGR"
     gene            complement(2068..>2340)
                     /locus_tag="PKH07_10595"
     CDS             complement(2068..>2340)
                     /locus_tag="PKH07_10595"
                     /inference="COORDINATES: protein motif:HMM:NF026064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HNT35432.1"
                     /translation="PSQDLNDLLDDVVAHHPPPSSHGKKEIIFYRLSQARVAPPTFVL
                     ETNQPDAIHFSYERYLLNQIYERFGFEGSPIVLRFKQRRRKRAKNS"
BASE COUNT          518 a          665 c          534 g          623 t
ORIGIN      
        1 agagtgtcaa catgacgtca acgagattca cgacgttgat ctccgtcatg gactgctttc
       61 gaagcctgcg acgcagtcga gctgcgctga cgttcatggt gtctgctgtt cctgttgttg
      121 gcgtccggcc cttctgagta tgctccgatc cagataactc gtcaggcggg acgcgaagtc
      181 ttccatctct tccagttgcc gttcgattcg acggttgaga tagttgtgac agactgccgc
      241 aggaatcgcc gcggccaatc cggccaccgt ggtgatcaga gcccctgcga tgccgggagc
      301 cacaacagtc agatccgttg taccggcaac tcccatgtca gtaaacgcca ccaacacacc
      361 ccaaacggta ccgaacagcc caagtaacgg cgagatatgg gctatcgttc ccagtatgct
      421 gatgtatcga tccatctgtg aaaccaagag tgcaatctga cggtctgctg ctcgttgaac
      481 caaatccacc atgcctggcg tcatcgccaa ctttccggtg tccggacgcg cgagcgcgcg
      541 aagctcccga tagacttccg aaaaaatcga ggccagtgga ctccctcggt gttcctgcgc
      601 ccgctcatgc gccaactgaa aatcgttgct gattgtgttg aactcagcta gaaatcttcg
      661 gttgttccac atcgatcgcc aaaactggag aatcttcagt cccgccaccg cccacaaaac
      721 caccgaaaag actaccaaca gaagcactat caacttccct ataggctctg tcgtcgtatt
      781 ggctcgccgc aaagtgtccg ccacccctgc ttgaaccgtg ctcattgtca ctaggctgcg
      841 aataatcaag gctcgaacct tatctcttca ggcgatcatc tgcccggaat tactatctcc
      901 tgacccactt ggatcttggc agcatctgta atgttgttca cccgaatcac ttcattggtc
      961 gaaacaccat atttctgcgc aatcgccgaa agtgtctctc caggagccac tttgtgaatt
     1021 gttccggagg ggtccgtaga aactcccgaa ctggttggcg caaccgtatt gtcactcgac
     1081 agtcctcgga tgatcttctc taaatcacga aaggatgtcg agacctcgtt tctcaacgcg
     1141 ttgatgcttc ctttattgcg ttcctcagaa cttgttatgt ctttggaaag cttggcttcc
     1201 aagtccccaa gagatttcct cgtctgcgct tgaaaatcag tcatctgctt gcccataccg
     1261 tcctgatagt ccttcgcttc tttctgaaga ctgtcttggg cccctttgag ttccgtcttc
     1321 agtacacctt cagccgagtc aattcgcgtc tccactcgtg ccactctcga cttcatatca
     1381 tccacttctc ggcgcatctt cagcacttcg gcagagagat cttctatcgc tgagttcatt
     1441 cgattctgat tgttctgtac caactcgttt tgcttcttca gtgtgctgta gagatcctgc
     1501 aggctctgtt ggctcttgtc actggagcgg tcgtagttct cgttgaatat ctccagacgc
     1561 gtttccaata ctcctatccg ctcttcgatt cgtcgcaact ggcctgtcgc acagccaaaa
     1621 ctcgagcaca tcaagacaat aactaacgct ttcttcattg tcggacgcat cggcaacttc
     1681 ctcttttctg gataaactct cgtgcggggg gctcacgccc ccttacgatg aacgacctct
     1741 cgaacaggtg gtcacgccct taccaccaat cgcattctct tatatcacta tcaataacca
     1801 atttgcaagg cttttttgtg aaaaatactt gcctctaagt gactagaaat ctgaaatcta
     1861 tgtctttttg gctcacacgg tttcgagcag gacagcaacc gttgccaatt tttctctctc
     1921 ttctcttttc ttctccacat cagacgatcc tcacagtcgg ataattcagg cttggatgag
     1981 acgattgcgt gcagtgcaaa aaccgcgata gacggcaatg gactccgatc ttcctgttgt
     2041 cagagtccgc ttctatcctg tatcactcta cgaattcttg gctctcttac gtcggcgttg
     2101 tttgaaccga agcacaattg gagagccttc gaatccaaaa cgctcataga tctggttgag
     2161 cagataccgt tcataggaga aatgaatggc gtccggctgg ttggtttcaa gtacgaaggt
     2221 cggcggcgcg acgcgcgcct gggagagtcg gtagaagatg atctctttct ttccatgcga
     2281 actgggagga ggatggtgtg cgacaacatc atcgagcaag tcattcagat cctgagaggg
//
LOCUS       DAOQUC010000606         2339 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_56462_length_2339_cov_3.286778, whole genome shotgun sequence.
ACCESSION   DAOQUC010000606 DAOQUC010000000
VERSION     DAOQUC010000606.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2339)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2339)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2339
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56462_length_2339_cov_3.286778"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..663)
                     /locus_tag="PKH07_10600"
     CDS             complement(<1..663)
                     /locus_tag="PKH07_10600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35433.1"
                     /translation="MADEQQNKTVVGADSERSQIGNVQDVLTLLFSESRPLQLQAIAA
                     AGQMGTPLLLRALLWKYADGVSIVSNAVDEALTPHLSRIRGILWREASSPMRRVRAAS
                     VHLLRQVAGLKDVGKFFPLLDDYELQVRDKTKQAIREVVGRELGSLENLDNLEERHQA
                     LSSLYRDFLALSASGRAKQQFGVEILVMIGVRSPNAFWHFLAETPGIDLNLFSGEFTR
                     GTD"
     gene            complement(673..>2339)
                     /locus_tag="PKH07_10605"
     CDS             complement(673..>2339)
                     /locus_tag="PKH07_10605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNT35434.1"
                     /translation="RTVHGNDSVSAPIQADSIDDVLRARISQLEKANEALRFSFGRLA
                     SLQEMARAISATHDLYEILEKVIGLVCDFLQARESVWLWFDSETLEYIPDQRLHGTNV
                     LAPAVQAYIEEGILDWALQQRRPAIISLSQTDPGGTDVLVPFVTSVGMSAVLHLETTL
                     EESCYEGQHFEILSLIQSHAATALENARLWGEMENFKDYTHNTIESLQQGVIVLNERN
                     EVTLANRVSLEIFGLLDSQVIGLNWVQIVPPEFLGTIDDLLTQALIQGRSEEEEITLD
                     RPDGSKSVAVTASLLKNARGDGLGLILVLRDLTESKEILELQKLDQLKTEFLSNVSHE
                     LRTPITSIKAYTETLLDQVESEDVETQKEFLRVVIQETERLSRLIEDLLDLSRIERGK
                     VEFHFERGDLQDVLQRAVAMLQPSAPLHQLQIVADASLEPFEFDRDKIHQVINNLLGN
                     AIKYSPRGGLIQVAVSPEGSQVHLSVQDSGIGITDEDLPKLFGRFFRAESPLKYEVPG
                     TGLGLVISQFIVHAHRGRIWVESEYGRGTTVHVVLPKKPAEAALQQ"
BASE COUNT          561 a          638 c          600 g          540 t
ORIGIN      
        1 gtctgttcct cgtgtgaact ctccagagaa aagattcaga tctatgcctg gtgtctctgc
       61 gagaaaatgc cagaaagcat tgggtgaacg aaccccaatc atcacaagta tctcaacacc
      121 gaattgttgc ttcgctcttc ctgaagccga aagcgccaag aaatcccggt acaatgaaga
      181 gagtgcttga tggcgttcct caagattgtc gaggttttcg agacttccca actcgcggcc
      241 aacaacctcg cgaatcgcct gcttggtctt gtccctgacc tggagttcat agtcatccaa
      301 aagaggaaaa aatttcccaa catccttgag tccggccacc tgcctgagca agtggacgct
      361 tgccgctctg acgcggcgca tcggactgga tgcttcgcgc cacaggatgc ctcgaatacg
      421 gctcaaatga ggagtgagcg cctcatccac cgcgttggaa acaattgaga cgccgtccgc
      481 atacttccac aacaatgccc tgagcaaaag aggcgttccc atctggcctg ctgccgcaat
      541 agcctgaagt tgaagtggcc gactctccga aaacaagaga gtcagaacgt cctgaacatt
      601 gccgatctgg cttcgttcag agtccgcacc aacaaccgtc ttattttgtt gttcgtctgc
      661 cattgtatct gtctactgct gtaacgccgc ttctgccggc tttttgggca ggacaacgtg
      721 aaccgttgtg cctctgccat actcactctc aacccagatt ctaccacggt gggcatgaac
      781 aatgaactga gagataacca aacccaagcc agtgccagga acttcatact tgagcggact
      841 ctccgccctg aagaaccttc cgaacagttt ggggagatct tcgtcagtga tgccgatccc
      901 gctgtcctga acggagaggt gcacctgcga gccctctggg gagacagcaa cctggatgag
      961 cccgccacga ggggagtact taatggcgtt gccaagaagg ttgttgatca cctgatgtat
     1021 tttgtcccgg tcgaattcga aaggttccag tgatgcatcg gccacgatct gcagttggtg
     1081 gagtggagct gatggctgga gcatggcaac agcacgctgg agcacgtctt gaagatcgcc
     1141 ccgctcgaaa tggaattcca ccttcccccg ttcaatgcgt gagagatcga ggagatcctc
     1201 tatcaatcgg gagagtcttt cggtttcctg aatcacgaca cgaaggaatt ccttctgcgt
     1261 ttcgacatct tcagattcaa cctgatcaag gagtgtttcc gtgtaggctt tgattgaagt
     1321 gatcggggtt cgcaactcat gggatacatt ggagaggaat tccgtcttga gttgatccaa
     1381 cttctggagc tccagaattt ccttgctctc ggtcaaatct ctcaacacca aaataagacc
     1441 gagaccatcc ccccgagcat tcttcagaag agaggcagtc acggcaacgg acttggagcc
     1501 atcgggacga tcgagtgtga tttcctcttc ctctgaacgg ccttgaatga gcgcctgagt
     1561 gaggagatcg tcaatggtgc ccaaaaactc cggtggaaca atctgcaccc agttcaggcc
     1621 gatcacctga ctgtcaagca ggccgaaaat ctccagtgag acacggttgg caagcgtgac
     1681 ttcgttcctt tcattgagaa cgatgacgcc ctgttgaaga ctttcgatgg tgttgtgcgt
     1741 gtagtctttg aaattctcca tctcgcccca aagcctggcg ttttcaagag ctgttgctgc
     1801 atgactttgg atcagggaga gaatctcgaa atgctgacct tcgtagcaac tctcctcaag
     1861 tgttgtctcc aggtgaagta ccgcactcat tccaaccgaa gtcacaaacg gcaccaggac
     1921 atcagttcct cctggatcgg tctgtgacag ggagatgatc gcgggacgac gctgctgcag
     1981 tgcccagtca agaatgcctt cctcaatgta cgcctgaacg gcaggtgcca ggacgttcgt
     2041 gccatgcagc cgttggtcgg ggatgtactc cagggtttcg ctgtcaaacc acagccagac
     2101 ggattcccgg gcttgcagga aatcgcacac cagaccaatg accttctcaa ggatttcata
     2161 gagatcatgt gtggcgctta tcgctcgcgc catctcctga agactggcca gacggccaaa
     2221 agaaaaccgc agcgcctcat tggccttctc caactgagag atgcgtgcac gaagcacgtc
     2281 gtcaatcgaa tcggcttgga tcggagctga gacagaatca ttgccatgaa ctgtccgtc
//
LOCUS       DAOQUC010000607         2332 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_56728_length_2332_cov_3.013175, whole genome shotgun sequence.
ACCESSION   DAOQUC010000607 DAOQUC010000000
VERSION     DAOQUC010000607.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2332
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56728_length_2332_cov_3.013175"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(8..547)
                     /locus_tag="PKH07_10610"
     CDS             complement(8..547)
                     /locus_tag="PKH07_10610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35435.1"
                     /translation="MIDRKLEQAIVDIQEFIVLWNNFFHVLEEASRSEDTLGWDEQKD
                     TEFLKMKAQIAYRKQVLLDSLGKEDFNMAGDIKKVLAMCPSLDILSGESPIKINTIRG
                     LWHEVFIALNKFVGQLKQRRDAIAHVSYFSFRMNAILKSKALRYSVIGVVAAIILGAL
                     AMVIPFEELFTRTQGLFGR"
     gene            complement(676..>2332)
                     /locus_tag="PKH07_10615"
     CDS             complement(676..>2332)
                     /locus_tag="PKH07_10615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35436.1"
                     /translation="PAALMNSENALVTARQSKLAKIAAAEAAMTHALGAFASRYANDL
                     VLQGQTLLGQSKVSMDQKDYAASNALADQAQSIFKAAEETAWKTRFEEDIAKLDFHIQ
                     KAAEQGALEKAPDLYALSVATQGSAKTLATQGQYKAAEEQALLGLKQTQDTLEHLGSK
                     STEITQQLETRAEELHGLVSDEAGRQRLEQFVQFLGSVQHADRQEDFATVFNLEKQSG
                     DVFKSVRTSIEDHNLVDLANSAGQLLVDYQKSGLALVAADEWQATRDQLLQEQQLIGQ
                     KDYEDSRNTLSEILSRSQGMQEMAQTNAMNRLDTLRSKMNEIQQSGGSELLPGEFEQT
                     LQSYREAQQALESKDWPYIYDALKRAEQSVSVMDDRTKVTFLENRYKDLIRSHLQEKR
                     SLEKGFDSVLELPVDFYLQAKSSAQVDIYKYLQKDMRTNILSKRSSLLLDKVKNIEPP
                     PSMLAVHSLALDTFTELNIMARLFEKYGQYDQFGEEQRLIAVKQAFEHYEILKKKSSE
                     LERQVTPTIVSEQRKSSPLDSLFPRREARLRVNINKSAEGSSR"
BASE COUNT          536 a          665 c          553 g          578 t
ORIGIN      
        1 aataaaatca gcgtccgaaa agcccctggg ttcgagtgaa cagctcctcg aagggaatga
       61 ccatcgccag agcacccaag atgatggcag caacgacccc gatgacgcta tatcgaagcg
      121 ccttgctctt caggatagca ttcatccgga aactgaaata cgagacatgg gcaatcgcgt
      181 cacgacgttg tttcaactga ccgacaaact tgttgagggc gataaacact tcgtgccaga
      241 gtccacgaat agtgttgatc ttaattggag actcgccact gaggatgtcc agggacggac
      301 acatggcaag aaccttcttg atgtccccag ccatattgaa gtcttctttt ccaaggctgt
      361 ccaagagcac ctgttttcgg taggcaatct gcgccttcat tttcaggaat tccgtgtcct
      421 tctgttcatc ccaacccaac gtgtcctcac tgcgcgatgc ttcttccaga acatgaaaga
      481 agttattcca gaggacaatg aactcctgaa tgtccacgat ggcttgttcc agtttcctat
      541 caatcatctt gcctgtccgc ctctaccccg tgcgttattt ctcccctttg gagaacagac
      601 ccatagaaaa ggccagatat ccaagataca agaccgccaa gcccatgaca gcgacttcca
      661 actgaatgac ttcgttcatc gcgaactacc ttccgccgac ttgttgatgt tcacccgcaa
      721 cctggcttca cgcctgggga acaaagagtc cagaggggac gacttgcgct gttccgagac
      781 gattgtcggg gtgacctgtc tctccagttc ggagctcttc ttcttcagga tttcgtagtg
      841 ttcaaaagct tgtttgacag cgattaggcg ctgttcctca ccaaactgat catattgacc
      901 gtatttttca aacaaacgcg ccatgatgtt taactctgtg aacgtgtcca gcgccaacga
      961 gtgaaccgcg agcatggagg gaggcggctc aatgttcttg accttgtcca gaagcagcga
     1021 tgatcgcttg ctgagtatat tcgtcctcat atccttctgc agatacttgt agatatcaac
     1081 ctgggcactg ctcttggctt ggagatagaa gtcaacggga agttccaaca ccgagtcaaa
     1141 acctttttcc aaagaccgct tctcctgcaa atgtgaacga atcagatctt tgtatctgtt
     1201 ctccaggaag gtaaccttgg ttcgatcgtc catcacagaa acgctctgct cggctcgttt
     1261 caatgcgtca tagatgtaag gccaatcctt gctctccagc gcctgctgtg cctcgcgata
     1321 cgattgcagc gtctgctcga actccccagg cagaagttca gaaccaccgg actgctgaat
     1381 ctcgttcatc ttgcttctca aagtgtccaa gcggttcatc gcattggtct gcgccatctc
     1441 ctgcatccct tgggaacggg aaaggatctc cgaaagggta ttgcgcgaat cttcatagtc
     1501 cttctgtccg atcaactgct gttcctggag cagttgatcc cgcgttgcct gccactcatc
     1561 ggcggcaaca agggccagac cggacttctg atagtccacc aacaattgtc cagcggagtt
     1621 cgccagatcg accaggttat ggtcctcgat ggaagtccga accgacttga acacatcacc
     1681 actctgtttc tccagattga acaccgtggc gaagtcttcc tgccgatcgg cgtgttgcac
     1741 cgaaccgaga aactgtacga actgttccag tctctggcga cccgcctcat cactgacaag
     1801 tccgtggagt tcttctgcac gagtttccaa ctgctgagta atctctgtgc tcttgcttcc
     1861 aaggtgctcc agagtgtcct gcgtctgctt caagccaagc agcgcctgtt cctcagccgc
     1921 cttgtactgc ccctgtgtcg caagcgtctt cgcagaacct tgtgtcgcca cagataaggc
     1981 atagagatca ggagcctttt ccagcgcgcc ctgttcggca gccttttgaa tatggaaatc
     2041 cagtttggca atatcctctt caaagcgagt cttccaggca gtctcctcgg ctgccttgaa
     2101 gatactctgc gcctgatccg cgagagcgtt cgaagcggca tagtccttct gatccatcga
     2161 aaccttggac tgccccaaga gcgtctgccc ttgcagaacc agatcgttgg cgtagcgaga
     2221 ggcaaaagca cccagcgcat gagtcatggc cgcttcagcc gcagcaatct tggcgagttt
     2281 gctctgtcgt gccgtgacaa gggcattctc agaattcatc aacgccgcgg ga
//
LOCUS       DAOQUC010000608         2332 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_56737_length_2332_cov_2.581028, whole genome shotgun sequence.
ACCESSION   DAOQUC010000608 DAOQUC010000000
VERSION     DAOQUC010000608.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2332
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56737_length_2332_cov_2.581028"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(200..1426)
                     /locus_tag="PKH07_10620"
     CDS             complement(200..1426)
                     /locus_tag="PKH07_10620"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNT35437.1"
                     /translation="MPRFEYKAKKATGEIIEGVIEAESRRAVISKIQGMRCFPLKVQE
                     QMDEGAFEKQVSMKMFERVRAKDVTVFTRQMSDLLKAGLPLVRALAVIETQTSKDKFR
                     RVVEDVRNGVQGGLAFSDALAKHPSIFPDLYSTMVRAGETGGMLETVLERLADFAEEQ
                     DEMKGRIMSAMAYPILMLCVGMLAILVLLTFVIPKFQGMFEDMGQTLPAPTQMLVLVS
                     SAVKNYWWIVAGLIALGVFSLKKFASTEEGKTALDRIRLRLPIFGDLALKRDVARFAR
                     TLGTLLNNGVPILKALNIVRATMRNRLLACIVDEVQQNIKEGEKLSEKLGESGLFPPI
                     AVNMIAVGEETGALETTLLRLAQSYEVGAERKIKTLTSLVEPAMILFMAVIVGCIVFA
                     MLLPVFDISGQLTNQG"
     gene            complement(1451..>2332)
                     /locus_tag="PKH07_10625"
     CDS             complement(1451..>2332)
                     /locus_tag="PKH07_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013537650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HNT35438.1"
                     /translation="FTNLISLPHGIVLVTGPTGSGKTTTLYGALDMLNKPEVKIVTVE
                     DPIEYQMRGITQVQVLPKIDLTFARILRAFLRQDPDIMLVGETRDSETAEITIRAALT
                     GHLVFSTLHTNDAAGAFTRLLDMGIESYLVASSVIGVLAQRLVRVICKSCKETCHPDP
                     VQLEGMGLPRNMASELELYRGRGCEACKFLGYKGRTGIYEIIPISERVKELTMERASA
                     NIIKKAALDEGMKTLRQDGWLKVLNGTTTIDEVLRVTQEDSFSDVGSAAGGEIKQMTD
                     AMAAQSLALKDRSTELT"
BASE COUNT          518 a          718 c          560 g          536 t
ORIGIN      
        1 gacttgggga gtagcaacaa ttcccaacac agcaacaatg agcaccacaa gcaagacctc
       61 aaaagggctc cacccgaaga gccatccaga ctgcaacggg cttctcgaac catttgcctt
      121 ccggccgttt ccgtttctct tcaatccttc aatcatcatg atctccgcct ctcttcggtg
      181 ccaaagacac cggtcccgtc tagccctggt tggtcaactg tccgctgata tcgaacacag
      241 gcagcagcat cgcgaacacg atacatccga caatgactgc catgaacagg atcatggcag
      301 gctcgaccaa cgacgtcagg gtcttgattt tacgctcggc gcccacttcg tacgactgcg
      361 ccagtcgaag cagcgtcgtc tccagcgccc ctgtctcctc acccaccgca atcatattga
      421 cagcgatggg cggaaacagg cccgactctc caagcttctc cgagagtttc tcaccttcct
      481 taatgttctg ttgcacttcg tccacgatgc aggcaagcag ccgattgcgc atcgtcgctc
      541 gaacaatgtt caaggccttc aaaatgggaa cgccgttgtt cagaagcgtt ccgagggttc
      601 gcgcaaaacg cgccacgtcg cgcttgagcg ccagatcgcc aaagatgggc agtcgaaggc
      661 gtattctgtc gagcgccgtc ttgccttctt cggtcgaggc aaacttcttc agactgaaca
      721 cccccagagc gatcagacct gcgacaatcc accagtagtt cttcacggct gagctgacca
      781 gaaccagcat ttgtgtcggc gcgggaagtg tctgccccat atcttcgaac atcccctgaa
      841 acttcggaat gacaaaagtc agcagaacga ggatggcaag cattccgacg cacagcatca
      901 gaatcggata tgccatcgcg ctcatgattc ttcccttcat ctcgtcctgt tcttcggcga
      961 aatccgccaa ccgctccaac acagtctcca gcatcccgcc cgtctctccc gcccgaacca
     1021 tggtcgagta gagatcaggg aatatgctcg gatgcttcgc cagtgcgtcc gaaaaggcca
     1081 gcccaccctg aaccccattg cgaacatcct cgacaacgcg ccgaaacttg tctttgctcg
     1141 tctgtgtctc gattactgca agcgcacgaa ccaagggcag acccgccttg aggagatcgc
     1201 tcatctgtcg ggtgaaaacc gtgacgtcct tggctctcac acgctcgaac atcttcatgg
     1261 agacctgttt ctcaaaagcg ccttcatcca tttgttcctg gactttcagc ggaaaacatc
     1321 gcatgccctg gattttggag atcacggcac gacggctctc ggcttcaatg acgccttcga
     1381 taatctctcc cgttgctttc ttggccttgt actcaaatct tggcattact cgaactcccg
     1441 atagggacga ttatgtcaac tcggtgcttc ggtctttcag cgccagagac tgcgctgcca
     1501 tggcatccgt catttgtttg atctcaccac cagcggcgct gcccacatcg gagaacgaat
     1561 cttcctgagt gacacgcaac acctcgtcaa tggtggtggt tccgttgaga accttgagcc
     1621 atccgtcctg tcgaagggtt ttcatccctt catcaagagc agccttcttg atgatgttcg
     1681 ccgaagcgcg ttccatggtc agttccttga cacgttccga aatggggata atctcataga
     1741 tacccgttct acccttgtat ccaaggaact tgcacgcttc gcacccacgc cccctgtaca
     1801 gttcaagttc cgaagccatg tttcgcggaa gtcccatgcc ttccaactgc actggatccg
     1861 gatggcacgt ctctttgcag cttttgcaga tgactcgaac caggcgctgt gccagcactc
     1921 cgatgaccga agacgccacc agataggact caatacccat gtccaggagc cttgtgaatg
     1981 cgcctgctgc gtcgttggta tgcagggtgg agaaaaccaa atgccccgtc agcgctgcgc
     2041 gaatggtgat ctcggcagtc tccgagtcac gggtttctcc caccaacatg atgtcagggt
     2101 cttggcgcag gaatgctctc aggattcgtg caaaggtcag atcaatcttc ggaagaacct
     2161 gcacctgggt gatgcctcgc atctgatatt cgatcggatc ttcaaccgtg acgattttca
     2221 cctcaggctt gttgagcatg tcgagggcac cgtacaaggt tgtcgtcttt ccacttcccg
     2281 tcggacctgt gaccagcaca atgccatggg gcagactgat caggttggtg aa
//
LOCUS       DAOQUC010000609         2331 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_56760_length_2331_cov_3.484183, whole genome shotgun sequence.
ACCESSION   DAOQUC010000609 DAOQUC010000000
VERSION     DAOQUC010000609.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2331
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56760_length_2331_cov_3.484183"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            381..>2331
                     /locus_tag="PKH07_10630"
     CDS             381..>2331
                     /locus_tag="PKH07_10630"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNT35439.1"
                     /translation="MLSTMRTHQKKILIAITFPIILVFVLYYGASKDMRQRRGESMDS
                     DYVALVNKDGRISRSRFLRAYSNNEQYLRNNYGEQWDETLVDRLELEKNVLDQLINEE
                     LLYQAAQEAGLKATDPEVISYIETTQAKPQGRTVREWLERLAMLGNDLEEYFDTQRRS
                     LLMTKMQRVITSNSHVTEQELTDAYRDREEKIAADYLAFEPKSYLTAVAEPTEQALKD
                     FLESNKERFRVPEKVKVKYLQIDPKIFRGTVQVTREAIERYYEDNAEEYEVPEQVQVE
                     HLAFFPEKFQDRVVLKEEDILSHFENNAGRYRTEPQMRVRYVALDFRQLMDTMSPTDE
                     EIQAYYEENPQGFYSEEERKVRRIQIPLADSDSGEQKTAKRELVRRIKTDIEEGQTFE
                     EAARQHSQDEFKDQGGLMGYRTYGDLASFGIADNVFELQPGAMSDVISVPNGLALVYV
                     EDIKEATIQPLYAVRDKVADVLKYNKAESESRQRLSDLREQLGKQIDPTQVPQGFIVR
                     ETQAFARGGNVDEVVGEDDLRTFTNEASRLSPGQISEPIPANKNWYLIEMLEKIDARD
                     MTLEEARERVAADKTREEAMKIAMQQAQDAEAMIRDGHGFDEISKSKGVDILDTGLLY
                     KEGRVPEIGWNAKDFLDTAFKLTDGE"
BASE COUNT          630 a          567 c          655 g          479 t
ORIGIN      
        1 aacagttttc gcgggcatag aagacagacc tcccaaaatc agactccaaa cgagcttgac
       61 ttgttcccag ggcaggcgtt cacagggatg gacacattcc gcacgcccgt tcacgctcgc
      121 cgactctggg caaattgacg ggatcataag caggaattcg ggtgagggtc aacttctatc
      181 ggaatcgccg gaaatttgct gaggatggct tgacaactgc attggggata cctattctat
      241 cgtgacattc ttcaggagcg gttggcactc gcgagcgtgt ggactgtgcc cactatcacg
      301 tgcgcttgct taaaccggaa agcagattcc tctggagtct gctctctcag gagagagttt
      361 aaggaaagag ggattctgta atgctcagta cgatgcgaac gcatcagaag aaaattctga
      421 ttgcaatcac cttcccgatt atcttggtct tcgtgctcta ttacggtgct tccaaggaca
      481 tgcgacaaag aagaggcgag tccatggact cggattacgt ggcgcttgtc aacaaggacg
      541 gccgcatcag ccgttcgcgc tttctgagag cctattcgaa caatgagcag tatctcagaa
      601 acaactacgg cgaacagtgg gatgagactc ttgtggatcg gttggaactg gagaagaacg
      661 ttctggacca gttgatcaac gaagagcttc tctatcaggc tgcccaggaa gccggtttga
      721 aggcgactga tcctgaagtg atctcctaca tcgagacgac gcaggccaag ccccagggaa
      781 gaacggttcg cgaatggctg gagcgtctgg ctatgctggg caacgatctc gaggagtact
      841 ttgacaccca gcgtcgcagc ctgctgatga ccaagatgca gagagtcatc accagcaact
      901 cgcacgtcac cgaacaagaa ctgacagacg cctatcggga tcgcgaggag aagatcgcgg
      961 ccgattatct ggcttttgaa ccgaagtcct atctgaccgc agtggcggaa ccgacggagc
     1021 aggcgctcaa ggattttctc gaaagcaaca aagagcgttt tcgtgttccg gagaaagtca
     1081 aagtcaagta tcttcaaatt gacccgaaga tttttcgggg taccgttcag gtcacgcgtg
     1141 aagccattga gcgctactat gaagacaatg cagaagagta cgaagttccc gaacaggtgc
     1201 aggtggaaca tctcgccttc ttccccgaga agttccagga cagggttgtt ctcaaggaag
     1261 aggacatcct gagtcatttt gagaacaacg cagggcgata ccgaaccgag ccgcagatgc
     1321 gtgtgcgcta tgtcgcgctg gacttccgac agctgatgga cacaatgtca ccgaccgatg
     1381 aggagattca ggcctactac gaggagaatc cccaaggctt ttactccgaa gaggaaagaa
     1441 aggttcgcag aattcagatc cccttagccg actcagacag cggagaacag aagaccgcca
     1501 aacgggaact cgtacgcagg atcaagaccg atatcgagga aggacaaacc ttcgaggaag
     1561 cagcccgtca acattcccag gatgaattca aagatcaagg gggtctgatg ggttatcgaa
     1621 cgtacggtga cttggcatca ttcggaattg cggacaatgt ctttgagttg cagccaggtg
     1681 caatgtccga cgttatcagc gttccgaacg gtctcgcctt ggtctatgtg gaagatatca
     1741 aagaagccac aatccagccc ctttacgcag tgcgagacaa ggttgctgac gtattgaagt
     1801 acaacaaggc ggaatctgag tctcgccagc gactcagcga tcttcgcgaa cagctgggta
     1861 aacagatcga tccgactcag gtgcctcaag ggttcatcgt tcgtgaaaca caggcatttg
     1921 cgcgaggtgg aaatgtggat gaagttgtgg gcgaagatga tctccgaacc ttcaccaacg
     1981 aggcctcacg cctgtcacca ggacagatca gtgagccgat tccagccaat aagaattggt
     2041 atttgattga gatgcttgag aagatagacg cccgtgacat gacgctcgaa gaagcccgag
     2101 aaagagtcgc ggcggacaag actcgtgaag aggcgatgaa gattgccatg cagcaggcgc
     2161 aagacgccga agccatgatt cgagacgggc atggctttga cgagatatct aagtcaaagg
     2221 gtgttgacat actcgatacc ggtcttctct acaaagaggg acgcgtacca gagatcggat
     2281 ggaatgccaa ggatttcctc gacaccgcct tcaagttgac ggacggtgag a
//
LOCUS       DAOQUC010000610         2329 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_56833_length_2329_cov_3.200528, whole genome shotgun sequence.
ACCESSION   DAOQUC010000610 DAOQUC010000000
VERSION     DAOQUC010000610.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2329
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56833_length_2329_cov_3.200528"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(271..1644)
                     /locus_tag="PKH07_10635"
     CDS             complement(271..1644)
                     /locus_tag="PKH07_10635"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNT35440.1"
                     /translation="MFKKKPKEDPRQSAPTRCCGGDKSVVVRRTLPVRHEVDVFVAGG
                     GPAGCAAAVAAARQGQRVFLAEGHSCLGGMGTAGMVPAFMQFSDGVNFLADGIGREIL
                     ENLRAIGGAGPDNAYGIHAESLKRVYDDLLENAKVEFSLHSTVIDVETHGDQVTAAIL
                     AGKSGLFAVKAGVYVDGTGDGDICAWAGAPFEKGDEEGTMMPGTLCSLWASVDWQTVR
                     GSGADRRELKQAIDDGIFTIPDVHLPGMWRVGETLGGGNIGHTFGVDSTDERSLTKAL
                     VWGRKLVLEFERYYKQYLKGYEKMELVGTGSLLGIRESRRIIGDHILCLEDFQKRAVF
                     EDEIGRYCYPIDIHPSKPGSNEAYKEFEKLFRDMRYAKGESYGIPYRVLTPKGLSNVL
                     VAGRCVSTDRYLQGSIRVMPGCYITGQAAGVAAAIAVERKCDTRGVAVGELQQRLKEM
                     GAYLPNA"
     gene            complement(1729..>2329)
                     /locus_tag="PKH07_10640"
     CDS             complement(1729..>2329)
                     /locus_tag="PKH07_10640"
                     /inference="COORDINATES: protein motif:HMM:TIGR01037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HNT35441.1"
                     /translation="EQYVQVAEAVCAAAIASAIELNVSCPNVKQGGLAFGKDCDTLKA
                     VVSAVRQKCTLPLWVKLTPNVTDIGSLARAAEEAGADALVAVNTFVGMALDIERGAPA
                     LGNRIGGLSGPAIKPLALYAVDQVVRATGIPVVAVGGISSGRDALEFMALGAVAFQIG
                     TSLMIDPCCPLAILSEMQEILAEKGLSSIQDWIGIAKAW"
BASE COUNT          520 a          735 c          571 g          503 t
ORIGIN      
        1 tgccaaacaa caggacttcg gatgccgaac agctggctca ggagttgagg ctgcccatga
       61 ctcagattgg gacgatcctc gaagcgtcag aaggctttca gtggcttgat tcatcgggcg
      121 ggaaaatggc cgttctgcgg tcggggtttg agcattttca cagggatcta tagtgcgaag
      181 cactgcaccg ttccgttctt cctcatcatt gtgcgctttg acggaagccc tcagataaac
      241 acacgtaaag ccagttggag cagaaacctt tcaggcgttg ggcaggtaag cgcccatctc
      301 cttaagtcgc tgctgcaatt cacccactgc aacacctcgt gtatcacact tgcgctccac
      361 cgcaatggct gcagcgacac ctgccgcctg accagtgata taacagcccg gcatgacacg
      421 aatggatcct tgaaggtagc gatccgtgct gacacatcgt cccgccacca gcacgttcga
      481 taagcctttg ggagtcagca cgcggtaagg aattccataa ctctcaccct tggcgtagcg
      541 catgtcacgg aagagctttt cgaattcctt gtacgcttca ttcgagcccg gcttggaagg
      601 atgaatgtca atgggatagc agtatctccc gatctcgtcc tcgaacacgg cacgcttctg
      661 gaagtcttcc agacagagaa tgtgatctcc gatgatccga cggctctcgc gaatccccag
      721 cagagagcct gttccaacca gttccatctt ttcgtatccc ttgagatact gcttgtagta
      781 gcgttcgaac tccaacacca gtttgcgacc ccagaccagc gccttggtca gagagcgctc
      841 atcggtggag tcaacgccaa aggtatgacc aatgtttcca ccccccagag tctcacccac
      901 acgccacatc ccaggaagat gtacgtcagg aatggtgaaa atgccatcat caatcgcttg
      961 cttcagttcc cgacgatcgg caccggaacc tcggacggtc tgccagtcaa cactcgccca
     1021 cagactgcac agggtgccgg gcatcatggt tccttcttcg tcacctttct caaatggcgc
     1081 tcctgcccag gcacagatat caccgtcgcc tgtcccgtcc acatagacac ccgctttgac
     1141 cgcaaacagg ccgctctttc cggcaaggat ggccgctgtc acctggtctc catgcgtttc
     1201 cacatcaatg actgtcgagt gcagcgagaa ttcgaccttg gcattctcaa gcagatcgtc
     1261 gtagacgcgc ttcagggatt ctgcatgaat tccataggca ttgtccggcc ctgcgccacc
     1321 gatggccctg agattctcca gaatctcacg accgattccg tctgccagga aattgacgcc
     1381 gtcagaaaac tgcatgaatg ccggtaccat acctgccgtt cccattccgc ccagacaact
     1441 gtgtccctcg gcgagaaaga ctctttgccc ctgtcgtgcc gcagccactg cagccgcgca
     1501 accggcaggc cctcctcccg ccacgaacac atccacctca tgccgaaccg gcaacgttct
     1561 tcgaacaaca acagacttgt ctccgccgca gcaccgagtc ggggctgact gtcttggatc
     1621 ttccttgggc tttttcttga acatgatggt tttccttttc agaccgttat tcaaaacaga
     1681 ggtgggagag atttctctgc cacaggatat ctaccgatca gaacgttatc accacgcctt
     1741 ggcgatcccg atccagtcct ggatgctgct cagtcccttc tctgcgagaa tctcctgcat
     1801 ttccgaaagg atggcaagcg gacagcaggg atcgatcatg aggctggttc cgatttggaa
     1861 agccaccgct ccaagtgcca tgaactcgag cgcatcccgc cctgatgata ttcctcccac
     1921 cgcgacaacc ggaatgcccg tggctcgaac aacctgatcc actgcgtaga gcgcaagtgg
     1981 ttttatggcg ggtccggaca aaccgccgat acggttgccc agcgcgggag caccgcgctc
     2041 aatgtccaaa gccatgccca caaaagtgtt gactgccacc aaggcgtcgg ctcccgcctc
     2101 ttccgcagca cgagccaacg atccaatgtc cgttacgttg ggagtcagtt tcacccagag
     2161 aggcagcgtg cacttctgtc tcaccgccga aacaaccgcc ttgagcgtgt cgcagtcctt
     2221 gccgaaagca agccccccct gcttcacgtt cggacaggac acattcaact cgatggcgga
     2281 tgcgattgct gcagcgcata cagcctcagc aacctgaaca tactgctcg
//
LOCUS       DAOQUC010000611         2328 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_56890_length_2328_cov_2.712275, whole genome shotgun sequence.
ACCESSION   DAOQUC010000611 DAOQUC010000000
VERSION     DAOQUC010000611.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2328)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2328)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2328
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56890_length_2328_cov_2.712275"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..284)
                     /locus_tag="PKH07_10645"
     CDS             complement(<1..284)
                     /locus_tag="PKH07_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018195715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35442.1"
                     /translation="MEWIRSEGDHFIQGQSGKRFVAWGLNYDHDHVGRLLEDYWEGEW
                     ESVVEDFWEMKALGANAVRVLLQVAKFMESAEVPNSVALEHLSRLVDLAE"
     gene            complement(421..1023)
                     /locus_tag="PKH07_10650"
     CDS             complement(421..1023)
                     /locus_tag="PKH07_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020249000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNT35443.1"
                     /translation="MRKVLGFTLIELLIVVAIIGILAAIAIPNFMLSQIRAKVARVHG
                     DLRSIATALETYRVDWNEYPMCDLEHAWSQYPVYPYCMTTPTAHLSKKVLDDPFRDPR
                     HEALSHRMRYRYLSGHPIGFIGGSTSDTKARNRYPCKTWMLFSCGPNNAPGTGSLDDT
                     PELSFPDFIGGVYYDPSNGTVSIGDIYRLLEPAKFVNLAP"
     gene            complement(1166..2293)
                     /locus_tag="PKH07_10655"
     CDS             complement(1166..2293)
                     /locus_tag="PKH07_10655"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HNT35444.1"
                     /translation="MFRKQLTIFHVGEIPIRLDLSWFLILLLFSWSLATHFFPLEYRG
                     LHTSTYWFMGIAAALLLFTSVLLHELGHSLVGRHFGIKIRGITLFIFGGVADMAEEPY
                     SPKAEFLMAVAGPLVSAVLAVVFWLIAKGVVLFRLPLVFYGIAEYLSLANTFLLIFNL
                     LPGFPLDGGRVVRAALWKAMGSLTRSTRIASAIGRGLGITIALLGLVSLFFGGQISGI
                     WLVLIGLFLRRAAEASYQNLYIRSGLEGLRIKDFMTPRLVSIPAEISLAVAVQEFFLR
                     HPYHIYPVVQEGHVVGFIEDKIVKTISSDQWQAKSVLDVTTTDPFKSAVNPDDDAVKA
                     LVTMAQNGVGRVPVVSEGQLVGIISRRDIMEMIRIRMALTK"
BASE COUNT          660 a          630 c          546 g          492 t
ORIGIN      
        1 tcttcagcca gatcaacaag gcgactgagg tgctccaacg caacagaatt cggcacctcg
       61 gcagactcca taaacttggc aacttgaaga agaactctga ccgcattggc acctaaggcc
      121 ttcatctccc aaaaatcctc aacaacgctt tcccactctc cttcccagta atcctcaaga
      181 agccttccga cgtggtcgtg gtcatagttc aaaccccaag cgacaaagcg cttccctgat
      241 tgcccctgaa tgaagtgatc tccttcagac ctgatccatt ccatcttctt cactttggtt
      301 tttcctgacc cagaaacgga ccttctgcct gcacatgatg cagtaacaag aacaatcgtg
      361 acgagcaggc acatcgccaa aaaattgaca gggagaaccg acttgcttcg aacacccgct
      421 ctaaggggcc aggttaacaa acttcgccgg ttcaagaagg cggtagatgt ctccgatgct
      481 gactgtgcca ttgctgggat cgtaatatac gccaccaatg aagtcgggga aagacaattc
      541 tggagtgtca tccagagagc cggttcccgg agcattgttc ggtccgcatg aaaagagcat
      601 ccatgtcttg cacggatatc gattgcgcgc ctttgtgtca gatgtcgagc caccgatgaa
      661 tccgattggg tgcccgctaa gataacggta cctcattcgg tgactgagcg cttcgtgtct
      721 cggatcacgg aatggatcgt ccaacacctt tttggagaga tgtgctgtgg gtgtagtcat
      781 gcaataggga tagactggat actgtgacca agcgtgttcg agatcacaca tgggatactc
      841 attccagtcc acacgatatg tctcaagagc cgtggcaatt gagcggagat ctccatgaac
      901 tcgcgcaacc tttgcgcgaa tctgactcag catgaaattg gggatggcta tggcagcaag
      961 tatcccgata attgccacaa caatcagtaa ctcgatcagt gtaaaaccca atacttttcg
     1021 catgatagcc tccaaaagat ataccaacgt tggtataact taatcgaatc aacagcaaat
     1081 gtcaagaaaa cgcaatttct tggcctcatt gacaaatgaa gaagtgagct gagcaatcaa
     1141 ctcgatcaag gaggatcatg aaggactatt tcgtcagtgc catgcgaatg cgaatcatct
     1201 ccatgatgtc ccgtctcgaa atgatcccca ccagctgtcc ctcggagacc accggtacac
     1261 gaccgacgcc gttctgagcc atggtgacca aggctttgac tgcatcatca tccggattga
     1321 ccgcgctctt gaaagggtca gtcgttgtca cgtcaagcac actcttggcc tgccattggt
     1381 cggaagagat cgtcttcacg atcttgtcct cgatgaaccc caccacatgg ccttcctgaa
     1441 caacggggta aatgtggtac ggatgcctca ggaagaactc ctgaacagca accgcgagtg
     1501 agatttctgc tggaattgag acaagtctcg gtgtcataaa atccttgatc cggagtcctt
     1561 ccaaaccgct tctgatgtag aggttctgat agcttgcctc tgccgctcgt ctcagaaaca
     1621 ggccaatcag aacaagccag atgcctgaaa tctgaccgcc aaagaagagg ctgacaagac
     1681 ccaacagagc aatcgtgatg cccagtcctc taccaatggc actcgcaatt cgggtagaac
     1741 gagtgagact gcccatcgcc ttccacaggg cagccctgac aaccctacca ccatcaagcg
     1801 gaaaccctgg caatagattg aaaatcaaaa ggaaggtatt tgccaaggaa agatactcag
     1861 cgatgccgta gaataccagc ggaagtcgaa aaagcacgac gcctttcgcg ataagccaaa
     1921 acacaacggc caagacagcc gagaccaacg gccccgcaac tgccatcaga aactcagcct
     1981 tggggctata tggctcctca gccatatcgg caacgccacc gaaaatgaaa agggtgatac
     2041 cacgaatctt gatgccgaaa tgcctgccga caagactgtg tcccaactcg tgaagcagaa
     2101 ccgacgtaaa gagcagtaga gcggcagcaa tgcccatgaa ccagtaagtg gaagtgtgaa
     2161 gtccccgata ctcaagtggg aagaaatgcg ttgccaacga ccagctgaac agcagcagga
     2221 tcaagaacca actcaagtcc aatcgaatag gtatctcacc gacgtgaaaa atggtcagct
     2281 gtttacgaaa cattcgtacc gcttatctgc tgaatccagc ctgtcttg
//
LOCUS       DAOQUC010000612         2323 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_57071_length_2323_cov_3.239859, whole genome shotgun sequence.
ACCESSION   DAOQUC010000612 DAOQUC010000000
VERSION     DAOQUC010000612.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2323
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57071_length_2323_cov_3.239859"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..679
                     /locus_tag="PKH07_10660"
     CDS             <1..679
                     /locus_tag="PKH07_10660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HNT35445.1"
                     /translation="LVTGPTGSGKSTTLASMIDLINSERSCHIITIEDPIEYLHQHKT
                     SVIEQREVGADTSSFTSALKYVLRQDPDVILIGEMRDLETIAAAITAAETGHLVFATL
                     HTTDAAQTVDRIIDVFPSYQQQQIRMQLASTLEGVLAQQLLPKSSGKGRVCAMEVLIG
                     TPAVQNLIREEKTQQLYATMEASNRVGMQTLDRALATLYKRGLINYEMALQKAHKKEE
                     MIRVLTM"
     gene            809..1489
                     /gene="yqeK"
                     /locus_tag="PKH07_10665"
     CDS             809..1489
                     /gene="yqeK"
                     /locus_tag="PKH07_10665"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: protein motif:HMM:TIGR00488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HNT35446.1"
                     /translation="MEAVLLQSGSLSVGDGLSISSQVGVQDVFDDIRRDLADLLDAER
                     YRHSVRTARVCRVLARRYGVDEERAYLAGLLHDCGKGRSLEILYGERARELERLDALT
                     LETRALHHSFIGAHLSRTRFGVEDAEILDAICFHPTGSDDFGLLGRILYLADYLEPGR
                     KFPDRKKLLDLAFDDLNGVLIEVIERRTTHLKSKGRAVHPRTEELWRVVQEEANPVEC
                     MIRNSTNG"
     gene            1606..1950
                     /gene="rsfS"
                     /locus_tag="PKH07_10670"
     CDS             1606..1950
                     /gene="rsfS"
                     /locus_tag="PKH07_10670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HNT35447.1"
                     /translation="MEIVRGAMQAAEDKKATNPVVVHIGEISAFADYFLVCSADTRIQ
                     LNAICDQIQKALGSQGLYPSHVEGYGQSGWVLMDYGTVIVHVFLEHLRSYYALEDLWG
                     DARRVNLREPDR"
BASE COUNT          549 a          536 c          726 g          512 t
ORIGIN      
        1 gctggtgacc ggaccgacag gtagcggaaa gagcacgacg ctggcttcca tgattgatct
       61 tatcaacagc gaacgcagtt gccacatcat cacgatcgag gatccaatcg agtatcttca
      121 tcagcacaaa acgtcggtca tcgagcagcg cgaagtggga gcggatacct cgtcgtttac
      181 ttcggcactg aagtacgtgc tgcggcagga tccggacgtt attctcattg gtgaaatgag
      241 ggatttggag accatcgccg cggcgatcac cgcagccgag acaggtcact tggtcttcgc
      301 gacactccac acgacggatg ctgcgcaaac ggttgatcga atcatagacg tttttccatc
      361 ctatcaacag cagcagattc ggatgcagct ggcttcgaca ctggaagggg ttcttgctca
      421 gcaacttctg ccgaaatctt caggaaaagg ccgagtgtgt gcgatggaag tgctcatcgg
      481 aacgcctgct gtgcaaaacc tgattcgcga agaaaagacc cagcaactgt acgccacgat
      541 ggaagcaagc aaccgcgtcg ggatgcagac gctcgatcgc gcgcttgcca cgctctacaa
      601 gcgtggtctg atcaactacg agatggcgct ccagaaggcg cataagaagg aagagatgat
      661 tcgggttctg accatgtagt gcctccgatc gtctcctgct ttggttttga ggaaaaggag
      721 tgagcaggcg atacccaagc gggtacttca gacaggacaa gagtgattct actgcgggat
      781 gcagtcttca ttccagtgcc agcactccat ggaggcggtg cttttgcagt cggggagtct
      841 gtctgttggg gatgggctca gcatctcgtc acaggtagga gttcaggacg tgtttgacga
      901 tatcaggcga gacttggcgg acttgttgga cgcagaaagg tacagacaca gcgtccggac
      961 ggcacgggtg tgccgagttc tcgcgaggcg atacggcgtg gatgaggaac gagcctatct
     1021 ggcagggctt cttcacgact gtggaaaggg aagatccttg gagattctct atggagagcg
     1081 ggcaagggaa ctggaacggc ttgacgctct gaccttggag acaagggcac tgcaccactc
     1141 attcattggt gctcatctct cgcgaacgcg ttttggggtg gaagacgcgg aaatcctgga
     1201 tgctatctgt ttccatccga ccggatctga cgattttgga ctactgggtc ggattctgta
     1261 tctggcggat tatcttgagc cgggaaggaa atttcccgac cggaagaaac ttctcgacct
     1321 ggcgtttgac gacctcaatg gagtgctcat cgaagttatt gagcgacgga cgacgcatct
     1381 gaaatccaag ggtcgagctg ttcatccaag gacagaagag ctttggcgag tcgtgcaaga
     1441 ggaagccaat ccggtcgaat gtatgatcag gaattcgacc aacggttgag ggttttccat
     1501 gcggtaagtg tgaagtcctc agacgttgtt gttcaaggtg agagactgcg atggggttcg
     1561 aggtgacgag agatggaacg aaagaggtga tcactgatcg agcctttgga aatcgttcgt
     1621 ggcgccatgc aggctgcgga agacaaaaag gcaacgaatc cggtggtggt tcatatcggg
     1681 gagatttccg cttttgcgga ctatttcctg gtgtgcagtg cggacactcg gattcagttg
     1741 aacgcgattt gcgaccagat tcagaaggct cttggaagcc agggtttgta ccccagccat
     1801 gtcgagggat acggtcagtc gggttgggtg ctgatggatt acggaacagt gatcgttcat
     1861 gttttcctgg agcatctgag gagttattac gcgctggagg atttgtgggg agacgcccga
     1921 cgggtgaacc tgcgggaacc cgatcggtga gggcgagggc tgaatgagca gatggagaga
     1981 gtcggccggt cttacgagca ggagccagtc catggtttgg actggtctta ggcattttgg
     2041 gttcacgacg cagcgaacaa caatgaccat gagggtcatg tgtgaggata acccgtgcgc
     2101 gggtagtaat cggcgcgctt ctggcgcaca aaccatttga ggagacgaga aatatgttag
     2161 acatcaggaa aggactggag cgcaatcggt tgctgcgagt tttcatggtg gccgtgattg
     2221 ggcttcttat acccgctttt gcccaggcac gcatcacgct caagcaactc aatgacctga
     2281 ccatcgaacg tcaaggaatc atgccgcgca gtgagatcac gga
//
LOCUS       DAOQUC010000613         2322 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_57109_length_2322_cov_3.333480, whole genome shotgun sequence.
ACCESSION   DAOQUC010000613 DAOQUC010000000
VERSION     DAOQUC010000613.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2322
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57109_length_2322_cov_3.333480"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            114..779
                     /gene="purN"
                     /locus_tag="PKH07_10675"
     CDS             114..779
                     /gene="purN"
                     /locus_tag="PKH07_10675"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010044007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HNT35448.1"
                     /translation="MQQSGSERVYTFPVLDRPIRLAVLLSGSGTTLQRFLDLITAGQL
                     CGEVCCVVSSRKDAYGLVRASQHGVPTLIAVRKEYPTMEEHSAAINDFLMPFQPDLIL
                     LAGFMVLYCPPQGMEMRVMNVHPALLPLFGGKGFYGHHVHEAVLNAGVKVSGATVHFV
                     DTTYDTGPIILQKPVMVEEDDTPDTLAERVQAAERDIYPEAVRLFSQGRLRVEGHRVR
                     ITS"
     gene            828..>2322
                     /locus_tag="PKH07_10680"
     CDS             828..>2322
                     /locus_tag="PKH07_10680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35449.1"
                     /translation="MYQAQRRGRWQGEGISPQSPLVKGGKTRNEPCYAHGGKTGNPSP
                     VEWGEAHDRSSRVEKDGLSHHLRMWLLISLLSLSYCAVLNAQEPATAFERLIRIGSQS
                     PSESSSVLEKDFLALDAYASGDLDRAVRIWGELVKERAGTDYSIEPLLCLLKIAERYT
                     GSAETVRSLAQDETLSVSPTNARMFRQALLDAATSITDATLARQSAQALGVITEWDRV
                     VGPFGHSGPMDFEERFSVERDPWALSYIGWLGDVTPQAATTSADGFLDFGSVLWGESG
                     VGYALVCLKADREGDTVLEIHSSAAVSCWWNGFRVMSSNPYDLDLRTRIRISVPVRPG
                     HNWLLVKCLSRSSGWSFRCALEGFGTWITKGELTRDTRVHPDYQTFLEPRLESPWMTE
                     NSLAQTLLPGDPSESLRWLTSIYRAVVAWHAGEFKIARRILDGMRESQPTCTLIHRLA
                     GDLLLSWAQQQTVSRTRLENEAADCYRKALDTDPGNVMASVGLALFHL"
BASE COUNT          502 a          599 c          684 g          537 t
ORIGIN      
        1 ggcacagtcc gttctggtcg ccaaaaagga ctgtgccggg gagcttcgag aagtgatccg
       61 aatgctggag tcccaagagg acttgaagaa atatctctaa aggggtttgc gcaatgcagc
      121 aatcaggcag cgagagagtc tatacatttc ccgttctcga tcgtcccatc cgactggcgg
      181 tgctgctgtc aggcagtgga acgacacttc agagatttct cgatctcatc actgcgggtc
      241 agctgtgcgg tgaggtgtgt tgcgttgttt catcccgcaa ggatgcctac ggactggtgc
      301 gggcttctca acacggtgtt ccgactctga ttgccgtccg caaagagtac ccgacgatgg
      361 aggaacacag cgctgccatc aatgatttcc tgatgccctt ccagcctgat ctgatccttt
      421 tggcaggctt catggtgctc tattgtccgc cgcagggaat ggaaatgcgc gtgatgaatg
      481 ttcatcccgc gctgcttcct ctgtttggag gaaaaggatt ctacggccat catgtgcatg
      541 aagcagtgct gaatgccgga gtgaaggttt caggcgcgac tgttcacttt gttgacacca
      601 cctatgacac gggaccaatc atcctccaaa aacccgttat ggtggaagaa gacgacacgc
      661 cagacaccct tgccgaacga gtacaagccg cagagcgaga catctacccc gaagcggttc
      721 gtttgttctc gcaagggcgc ttgcgtgttg aaggccaccg agttcggatc acttcgtgaa
      781 aaagccgcaa cacacagccg agcgttcgcc ggcttaagag gcactgaatg taccaagcgc
      841 aaagacgagg cagatggcaa ggagagggaa tctcccctca atcccccctt gtcaaggggg
      901 gcaaaacgag gaatgagccc tgctatgctc atgggggcaa gacaggcaat ccgtctcctg
      961 ttgaatgggg tgaggcacac gatcgatcct cgcgggtcga gaaggatggt ctgagccatc
     1021 atctcagaat gtggctgttg atttcgcttc tgtcactttc gtattgcgct gtcctgaacg
     1081 cgcaggaacc ggccactgct ttcgagcgtc tcatcaggat cggttcacag agtccttcag
     1141 agtcaagttc cgtcctcgaa aaagactttc tcgcgcttga tgcctatgcg tcgggtgatc
     1201 ttgatcgagc agtccggata tggggggaat tggtgaagga acgtgcggga acggactact
     1261 cgattgagcc gttgctttgc ctgctgaaaa tcgctgagag atataccggt tcagcggaaa
     1321 cggttcgatc tctcgctcag gatgagacac tttctgtctc cccgacaaac gcgaggatgt
     1381 tcagacaggc cttgcttgat gcagccacaa gcattacaga cgcgactttg gcaaggcagt
     1441 cagcacaagc gcttggtgtg atcacggaat gggatcgcgt tgtggggccg tttggtcact
     1501 ccggaccgat ggattttgag gaacgctttt ccgttgagag ggatccctgg gcgctgtcct
     1561 acattggatg gcttggagac gttacaccac aggccgcgac gacctcggcg gatggattcc
     1621 tggattttgg ttccgtgctc tggggcgagt caggcgtggg ctacgctctg gtctgtctga
     1681 aagccgatcg agaaggggac acggtcttgg aaattcattc ctccgctgcg gtctcctgtt
     1741 ggtggaatgg ctttcgagtg atgagcagca atccctatga cctggacttg agaacgcgta
     1801 tccgcattag cgttcccgtt agaccggggc ataattggct tctggtcaaa tgtctcagca
     1861 ggagttccgg ttggagtttt cgctgcgctt tggaaggctt tggaacgtgg attacaaagg
     1921 gtgagttgac acgagatacg cgagttcatc ctgactatca gacctttctg gaacctcgac
     1981 tcgaatctcc ctggatgact gaaaactcgt tggcgcagac gcttcttccc ggagatccat
     2041 cggaaagcct tcgatggctg acttccatct accgtgcagt tgtggcatgg catgcgggtg
     2101 agttcaagat cgcgcggcgt attctggacg ggatgcgcga gagccaaccc acatgcaccc
     2161 tgatacaccg gcttgcgggc gatctgttgc tgtcctgggc acagcaacag actgtctcca
     2221 gaacgcggct ggagaacgaa gcagcagact gctatcgcaa ggcgctcgac acagatcccg
     2281 gcaacgtgat ggcaagcgtc ggtctggcgc tttttcatct gc
//
LOCUS       DAOQUC010000614         2322 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_57125_length_2322_cov_2.826643, whole genome shotgun sequence.
ACCESSION   DAOQUC010000614 DAOQUC010000000
VERSION     DAOQUC010000614.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2322
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57125_length_2322_cov_2.826643"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..259
                     /locus_tag="PKH07_10685"
     CDS             <1..259
                     /locus_tag="PKH07_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HNT35450.1"
                     /translation="EWGDGIYGCDLAARTYFGSSPKGLLPEQAALLAAMLPNPRYLDP
                     RVRPEKMAWKRKWILQRLRSKGTLTQSEYRRAVGTRILVTK"
     gene            complement(297..1319)
                     /locus_tag="PKH07_10690"
     CDS             complement(297..1319)
                     /locus_tag="PKH07_10690"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel protein"
                     /protein_id="HNT35451.1"
                     /translation="MLTDKAMTPQKRLVVVSLLVAVVVTSGMCGYRLLNPDWTLLDSL
                     YMTVITLTTVGFSEVHPLDDASRIFTILLLSVGIGTFAYGLSTLVASLVEGQLRFLLW
                     QRRMEKKVKKVRDHFIICGLGETGSIVMEEFQTTNTSFVVVEKSPERIQKLVEAGILV
                     VAGSATEDKVLIEAGVERARGLVAALPSDEENVFTILTAREINPNLKIVSVSSSDEAE
                     PKVRRAGADYVVTPSKIGGMRMASVMIRPTVVSFLDIMMKGQDMSLRMEESEIPANSS
                     LCGKTLAEAAIPAKTGLMCVAIRKSKDKRYLYNPKSQTAIEPHDVLIVLGSTEQLEEL
                     RKYVVS"
     gene            complement(1328..>2322)
                     /locus_tag="PKH07_10695"
     CDS             complement(1328..>2322)
                     /locus_tag="PKH07_10695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35452.1"
                     /translation="LAFFIPDPLVHVRGTMMRFLGFHFVMPLSVAEETYLCFPVTPLS
                     KNAFAGYIPILLSAVACLYSVRNVTRWLLIASLSGVFALGPRLHVAGQQGITMPYILF
                     KYVPGLSMMRCPHHFTLLYLLALAVMAGVGIDIVSKCTKGGHRKVWLLAGVIVLLLAD
                     LSLAPNTFLEDVSDMRDSLQGIVSEEKATVLDIPTAFSVEEAWHASRYLYLQTFHHQP
                     IVCGYTSRYQGIDLHGVTNRFPVLRKVSVLASMPSSVDLQIEALKTSEASLTREDLEN
                     LRSACEQLGIRWILLHRTFVQESISKALKKALSDEFGTPEFVNERFAGFRVLPE"
BASE COUNT          648 a          610 c          569 g          495 t
ORIGIN      
        1 cgagtgggga gacgggatat acggatgcga tctcgccgcc agaacgtact ttggatccag
       61 tccgaagggg cttcttcccg aacaggcagc acttctggca gccatgcttc ccaatccgcg
      121 ctatctcgat ccgagagttc gacctgagaa gatggcatgg aaaagaaagt ggatcctgca
      181 gcgactccgc tcgaagggaa cattgacgca atcggaatac cggagagcgg tgggaaccag
      241 aattcttgtc acaaagtgac tgccgcccaa cctgagtggt gcgaggggga ttcgcttcag
      301 gaaacaacat acttgcggag ttcttccagc tgttcggtgc tacccaggac gataagaacg
      361 tcgtgaggct cgatggccgt ctgggacttg ggattgtaga gataccgctt atccttggat
      421 ttccgtatcg cgacgcacat cagacctgtc ttggcaggaa tagcggcttc ggcaagagtc
      481 ttgccacaca gtgaggagtt tgccggaatc tctgattcct ccatcctcag ggacatatcc
      541 tgccccttca tcatgatatc gagaaaggac accaccgtgg gacgaatcat gacagaagcc
      601 attctcatcc caccgatctt gcttggcgta acaacgtagt ctgctccagc acgacgtacc
      661 ttcggttcag cctcatcgga agatgaaacg gagacgatct tcagattcgg gttgatctca
      721 cgagcagtca agatggtgaa aacattctcc tcatctgatg gcaacgcggc gacaagaccc
      781 cgtgcgcgct cgacacccgc ctcaatgaga accttgtctt cagtggcact gccagcgacg
      841 acaagaatac ccgcttccac aagtttctga attctctcgg gcgacttctc cacaacaaca
      901 aatgacgtat tggttgtctg gaactcctcc atgacgattg aacccgtctc acccaatccg
      961 cagatgatga aatgatccct aactttcttc accttcttct ccattctcct ttgccacaga
     1021 aggaaccgca actgcccctc aacaagggaa gcaaccaaag tacttagtcc ataggcaaaa
     1081 gtacctatgc caacagagag aagcaggatt gtgaaaatcc tgctggcatc atccagaggg
     1141 tgcacttcac tgaaaccgac agtcgtcagg gtgataaccg tcatgtaaag actgtccaaa
     1201 agtgtccagt caggattcag aagacgatat ccacacatac ccgacgtgac aacaactgca
     1261 acaagaagac ttactacgac aagacgtttc tgtggtgtca tggccttatc agtgagcaca
     1321 tatcctctca ttcgggaagg acacgaaatc ccgcaaacct ctcatttaca aactctggag
     1381 tgccgaattc atcgctcaac gccttcttca atgccttgga gatactctct tgcacaaaag
     1441 ttctgtgcag cagaatccaa cgaataccca actgctcgca cgcagaccta aggttctcca
     1501 gatcttcgcg agtcagtgat gcctcacttg ttttcagcgc ctcaatctgc agatctactg
     1561 aagatggcat actagccaaa acggacacct ttctcaagac tgggaatcgg tttgtgacgc
     1621 cgtgaagatc aatgccctga tatctggacg tataaccgca aacgataggt tggtgatgga
     1681 aagtctgaag atagagatac cttgaggcat gccaggcttc ctcgaccgaa aatgctgtgg
     1741 gaatatcaag aacagtggct ttctcctcag agacaatccc ttgaagactg tctcgcatat
     1801 ctgacacatc ctcgagaaaa gtgtttggag caagtgaaag gtctgcgagt agaagaacga
     1861 tcacccctgc caagagccac acctttctgt gaccgccttt tgtgcatttt gagacaatat
     1921 caattcctac gccagccatc actgccagtg caagaaggta caacaacgtg aagtgatgtg
     1981 ggcatcgcat cattgaaagt cctggcacgt atttgaacag gatgtagggc atggtaatac
     2041 cttgctgtcc agcaacatga agtctgggcc ccaaggcaaa gacaccagac agcgaggcga
     2101 tcaacagcca acgggtcacg ttgcgcaccg agtacaggca ggcaacggca gacaaaagaa
     2161 tggggatata gcctgcaaaa gcgtttttcg agagaggggt aacaggaaag caaaggtagg
     2221 tctcttccgc aacggacaga ggcatcacaa aatggaatcc caagaaacgc atcattgtcc
     2281 cacgaacatg gacaagagga tcggggatga agaaggcaag ga
//
LOCUS       DAOQUC010000615         2317 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_57324_length_2317_cov_2.271883, whole genome shotgun sequence.
ACCESSION   DAOQUC010000615 DAOQUC010000000
VERSION     DAOQUC010000615.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57324_length_2317_cov_2.271883"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..685)
                     /locus_tag="PKH07_10700"
     CDS             complement(<1..685)
                     /locus_tag="PKH07_10700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35453.1"
                     /translation="MEKAKSINNNRKIIALAAEKNLSKNIFPVVSLKGKEISYPFMKQ
                     LKDQKMRCTAGKFGSKNLMIMDILGTYAIHWAYNRFDDGSISFTSRIPTDRNMKVKRI
                     SHAGISHKILSYTARLLPEYHDGRIPSYLYADNALPDSVDRIYKRIKKPLRIKLRDSY
                     LKEQLPFLGKYSSKEICELITNTGQCALEMTYPIRFFDGKKPQNFFFNNSGIPSTMYS
                     LLDVKAVKTS"
     gene            complement(698..889)
                     /locus_tag="PKH07_10705"
     CDS             complement(698..889)
                     /locus_tag="PKH07_10705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35454.1"
                     /translation="MKTRRFTQGITIFTTPEMYQAIKKITDEERMSISEFFRNVVNEY
                     LSQSGERYPYLNETDEKDA"
     gene            complement(1064..1564)
                     /locus_tag="PKH07_10710"
     CDS             complement(1064..1564)
                     /locus_tag="PKH07_10710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35455.1"
                     /translation="MKSKMSKKKIDNGKASAIFQQMKTCLLELGLKIHSEAASEGEMG
                     MHAKLDIEDVRAAFSLLYYPKDGVIALLAMHYDLTDYERHAIVCVIPNIHKPHDTIKP
                     CASLEIGLIGYMAELTVEHGSLNVQHFRQVLSGLVKMSRCISSFVCGLRDAREGTSKR
                     AVEILD"
     gene            complement(1608..>2317)
                     /locus_tag="PKH07_10715"
     CDS             complement(1608..>2317)
                     /locus_tag="PKH07_10715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35456.1"
                     /translation="NLVCMTETARLRRRYCPEIELFIPVQCYTLEQFGYIERHLVDLE
                     YNGLSLPTRNLDPGGIALFLLKFYKMGIRKVHLLSVSNFTGLALAAYFARHVFDWCSV
                     DATTWRLTAQYQEYLHPRDLSKTILRNNAVIQPRQLPCRCPWCSTKDFAQIQLLSVKD
                     KTGFLRQHNFHVIQELGCEFYARSANFDSYIQHLRERNQRQARKVRKLIEALSVVHYR
                     KNEPIETLRQVLGGGKL"
BASE COUNT          508 a          564 c          576 g          669 t
ORIGIN      
        1 ttgatgtctt gacggctttt acatcaagca aggagtacat ggttgacggg atcccgctat
       61 tgttgaagaa gaaattctgg ggtttcttgc catcgaagaa tcgaatgggg taagtcatct
      121 cgagtgcgca ttgtcccgtg ttggtaatca actcgcatat ttccttcgaa gagtattttc
      181 caaggaaggg aagctgctcc ttgaggtagg agtcgcgcag cttgattcgc aagggtttct
      241 ttatcctctt atagatgcga tccacactgt caggcagagc attgtcggcg tacaggtaac
      301 tggggatgcg cccgtcgtga tactccggga gcaggcgcgc tgtgtaactc aagatcttgt
      361 gggagatgcc agcgtgggaa atccgtttga ctttcatatt cctatccgtg gggattcttg
      421 acgtgaagct tatcgaacca tcatcgaagc gattgtatgc ccaatgtatg gcataggtgc
      481 ccaggatgtc catgatcatc aaattcttgg aaccgaactt tcccgccgta catctcatct
      541 tctggtcctt gagctgcttc atgaagggat agctgatctc tttgcccttc aggctcacga
      601 cggggaagat gttcttgctc agattctttt cggcggcgag tgcgatgatt tttctgttgt
      661 tattaatgct ttttgctttt tccatagcgc gctctcctta agcatccttt tcatctgtct
      721 cgttgagata ggggtatcgc tccccactct gactcaggta ctcattgacc acattccgga
      781 agaactcgga gatgctcatc ctctcctcgt cggtgatttt tttgattgcc tggtacatct
      841 ctggggtcgt gaaaatggta atgccttggg taaatcttct ggtcttcatg cgctgcctcc
      901 tatcgttatt gattttttag gtctttatta tttgttccct ctttcagaag aaacttccca
      961 ctcagaaaat cttgatccaa aatgctgaag ggaatagctc cctgaagtct attccctccg
     1021 ttggcatcga tctgcccaga acccgttcaa cctcatcctg caatcagtcc aatatctcaa
     1081 ccgctctctt cgaggttcct tctcgggcat cacgtagacc gcatacaaag ctcgagatgc
     1141 atcggctcat tttcactagc ccgctgagga cctgtcggaa atgctgaacg ttcagtgaac
     1201 catgctcgac ggtcagttca gccatgtatc caatgagtcc tatttctaac gatgcgcatg
     1261 gtttgatggt gtcatgcggc ttatggatat tgggaattac gcacacgatg gcatgccttt
     1321 cataatcggt aagatcgtag tgcattgcga gcaatgcgat tacaccatct ttgggatagt
     1381 atagaagaga aaatgcggca cggacatcct ctatatccag ctttgcatgc atgcccattt
     1441 ccccctcgct agccgcttcg ctgtgaatct tcagaccaag ttccaggagg caggtcttca
     1501 tttgctggaa gatggcacta gcttttccgt tatcaatctt tttcttggac attttcgatt
     1561 tcatatacag actccttttg attgattagt tgtgattatg gtcatcccta caacttcccg
     1621 ccacccaaca cctgccgcag ggtttcgatg ggctcgttct tcctgtaatg gacgacggaa
     1681 agtgcctcga tgagcttcct gactttccgt gcctgtcgtt gattcctctc cctgaggtgc
     1741 tggatgtagg agtcgaagtt tgcggaccgg gcatagaact cacaccccaa ttcctgtatg
     1801 acatggaagt tatgctgcct cagaaatccc gtcttatctt tgacggatag gagttggatc
     1861 tgagcgaagt ccttggtgga acaccaggga catcgacagg gaagttgtcg cggttgaatg
     1921 acggcgttgt ttctcaggat cgtcttggaa agatcccgcg ggtggagata ttcttggtac
     1981 tgcgccgtca atcgccacgt tgtggcatca acggagcacc agtcgaacac gtggcgagca
     2041 aaatacgcgg caagggctaa ccccgtgaag ttcgagacgc tgagaaggtg gacctttcgg
     2101 atccccatct tgtagaactt cagaaggaat agggctattc ctccaggatc gagattccgc
     2161 gtggggagac tcaatccgtt gtactccagg tcaacgagat gcctttcgat gtagccgaac
     2221 tgctcgagtg tgtagcactg gaccgggatg aagagctcta tttccgggca atatctcctt
     2281 ctaagtcttg cggtctcggt catgcagacg agattga
//
LOCUS       DAOQUC010000616         2316 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_57353_length_2316_cov_2.975232, whole genome shotgun sequence.
ACCESSION   DAOQUC010000616 DAOQUC010000000
VERSION     DAOQUC010000616.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2316
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57353_length_2316_cov_2.975232"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..608)
                     /locus_tag="PKH07_10720"
     CDS             complement(<1..608)
                     /locus_tag="PKH07_10720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35457.1"
                     /translation="MSAENAVRKYFDPEEPILLLTDSDIGTDDKFGKEWLAVTDRRLV
                     VFTDGQTDRPRLDLALKDVENFQNVHVVGAIALRAEMKDKRIEIVRFTNSLSSKYSRI
                     VKALNDAQKNEKPPEFKLEDEERLNCPECGRLLPEKGSFCPACLKKRKVLERFWRYMK
                     PHWRRAIAVSTCIVASSVLELAPPYLVKLLVDDVLTQKAESVT"
     gene            820..1896
                     /locus_tag="PKH07_10725"
     CDS             820..1896
                     /locus_tag="PKH07_10725"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNT35458.1"
                     /translation="MAERTRRKNPTSKDIAQRLGINQSTVSRALHGKCASPEMVAKVR
                     KVADELGYRPHAGARLLVSGRSHSICLMFPDLSDHYQFQSIAAFHREARKLGYHIVLA
                     PYERDDTEFEEYGDYLVRERRVDAVIAYGIIHPRFDAVVARLMSQNVPTLTIGPCHVP
                     NVHLLNIDYDQFRRDVANHLANLGHTTIGVILNTIGDLKGVEAEHLHRMHVFQNTLHA
                     RGISWSRGDLLQMCIPTIENGYQAARHLLELHPRPTAIYSSKDVLALGVLQAARDMGL
                     RVPQDLSITGFENTELSQFFDITTGECSLDEVASKCLRIVIEVLTRGSDNLGVLYDEV
                     KPVLIIRGSTGVAPRNSNPERRQH"
BASE COUNT          573 a          573 c          625 g          545 t
ORIGIN      
        1 gttacgcttt cggccttttg ggtcaggaca tcgtcaacca gcagtttgac aaggtaaggt
       61 ggtgccaatt ccagaacgga ggaggcgaca atgcatgtgc tgacagcaat agcgcgccgc
      121 caatgtggtt tcatataacg ccaaaaacgc tcgagtacct ttcgtttctt caggcacgca
      181 ggacagaaac tgcctttctc aggcagcaac ctgccacact ccgggcagtt gaggcgttct
      241 tcatcctcca acttgaactc cgggggcttt tcgttcttct gagcatcatt gagcgctttg
      301 acaattctgg aatacttgga ggacaacgag ttcgtgaaac gaacaatttc gatgcgcttg
      361 tctttcatct cggcacgtaa agcaatcgca cccaccacgt gaacattttg gaaattctcg
      421 acatctttca gggcaaggtc gagacgtgga cgatctgtct gtccgtccgt aaacaccacg
      481 agacgccgat cggtgaccgc aagccattcc ttaccgaatt tgtcgtcggt gccgatgtct
      541 gagtcggtca ggagaagaat cggctcttcg ggatcgaagt acttgcgaac agcattctct
      601 gcggacatat cgaacgactc ctcttatcag gacaatgatg cagattacac ttcagggcat
      661 tgacacgcaa gttgacgtcg gatatcccat tcgcacgata tgtgaggaag gggcggtctg
      721 tcgtcttcaa tcaacgcttg tatgtatagt ggtgatgtcc tatgttagtg ccatagtcta
      781 cccagtcagg tggcaaagaa cggagcgcag ttactgcata tggcagaacg aactcgacgc
      841 aagaatccta ccagcaagga cattgcccag cgcttgggga tcaaccagtc cacggtgtcc
      901 cgtgcacttc atggcaagtg tgccagcccg gagatggttg ccaaagtcag gaaggttgca
      961 gatgagttgg gttacagacc tcacgcaggg gcgcgccttc tcgtctcagg caggagccat
     1021 tccatttgcc tgatgttccc cgatctttcc gatcattatc agtttcagtc catcgcggcc
     1081 ttccatcgtg aggcacgaaa gctcggctac cacattgttc tggcacccta tgagcgagac
     1141 gacacagagt ttgaagagta tggagactac cttgttcgcg agcgaagagt ggacgcggtt
     1201 attgcgtacg gaatcatcca ccctcgcttt gatgccgtgg tggcaaggct gatgagccag
     1261 aacgtcccca ctcttaccat tggtccctgc cacgttccaa atgtccatct tctgaacata
     1321 gactatgatc agttccggcg cgatgtcgcc aaccaccttg cgaatcttgg gcacacaaca
     1381 atcggagtga tcctcaacac gataggagat ctcaaaggtg ttgaggcgga acacctacat
     1441 aggatgcatg tctttcagaa cacgcttcat gccagaggga tttcctggtc gcgcggcgat
     1501 cttcttcaga tgtgcatccc cactatcgag aatgggtatc aagcggcgag gcatctcctc
     1561 gaactgcatc cgcgaccaac cgcaatctac tcaagcaagg acgtgcttgc ccttggtgtg
     1621 ctgcaggccg caagagacat gggactccga gtcccgcagg acttgtcgat cactggtttt
     1681 gaaaacaccg aactatcaca attcttcgac atcacaaccg gggaatgctc tctagatgag
     1741 gttgcatcga aatgcctgcg cattgtcatc gaggttctga caagaggcag tgacaacttg
     1801 ggcgttctgt atgatgaggt gaagcccgtt ctgatcatca gaggctccac aggtgtagca
     1861 cctagaaact caaatccgga gcggagacaa cactgaggca aaggcggtaa gaaccttcca
     1921 tggaaaggaa gcagcaaata tctcttgaca attgcatacg tatgcaatac actctgagaa
     1981 aaatgagatg ttgttgtcag tacgatgatt gaaccggcgt atatcctttt gtgctgtatg
     2041 tggacatgga gaggaggtgc ctcatctcga atctcaacga accgagagat attcttgaaa
     2101 gtgcgtgtat gaaaaagggt acttggatgt gtttggtggc actgagtcca aaagacgcag
     2161 agagcgtatc gagcagacgt ccaaaggaga gggcgtaggc caggcgtcag tgggcgcagg
     2221 tctactcaga aagaagggtg gatgcgatga gaatgatgcg cagggtattg ctggtggggt
     2281 tgctgatggt ttttaccgtt tcggtttggg ctgaca
//
LOCUS       DAOQUC010000617         2315 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_57400_length_2315_cov_2.676991, whole genome shotgun sequence.
ACCESSION   DAOQUC010000617 DAOQUC010000000
VERSION     DAOQUC010000617.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2315
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57400_length_2315_cov_2.676991"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..150)
                     /locus_tag="PKH07_10730"
     CDS             complement(<1..150)
                     /locus_tag="PKH07_10730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HNT35459.1"
                     /translation="MALTKAVKEKVILDHKQHDKDTGSPEVQIALLTERIRELTEHFK
                     VHIKDH"
     gene            469..>2315
                     /locus_tag="PKH07_10735"
     CDS             469..>2315
                     /locus_tag="PKH07_10735"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNT35460.1"
                     /translation="MSIRKRAFWIVGCLVIFPLWCGAVEDFSFIHVSDDHFPRDISAE
                     TYTEMAKRITPVQLAPYGITAPTPSFLFNTGDLTEFGGGRGVYKDLVRYLENLGIPFY
                     HVAGNHDNTWHSMRPAIKQRYGAPFYSFDQHGCHFIGLESAGIHEPLPALGREMLLFL
                     ENDLRKVSPDTPLFIFIHHKPDGSEFASRLERDRIVDLLRAYKSVLFLTGHGHSAAST
                     PFDDMDCTQGGSTYGPNDRGWVIATVVKDHLYVAFVPWGETQATRPLLERPLFGKPPY
                     LRIDIDHPRASQTFTKSLPLKVSWTGKDIQVASATYQIDDGDEVAITVAEGACRAEID
                     ISSLLNGAHYLRFNAKDSAAKSYHRTVVFYKETSDAPRALWRTTLLGSSKCTPTLSNG
                     MLYVGANDGFLYALDARNGKIQWKFETGLEVIAKPLLLKDSIFFGSADGNFYAVSSKG
                     KLLWKTPSESGDGFYSWPATDGKNIFSGCDDGYIYCFDAASGNKNWVFKDTGYAVESA
                     VCFADDVVYAGSWDRYIYALTPINGALKWKTLGSKSSTVTTGASRYYAPADNGPLAVS
                     EKMFCTDRGYELGYYDAKDGRLFKTIENVSAIALSEDQKSLYLRGNTNG"
BASE COUNT          562 a          629 c          566 g          558 t
ORIGIN      
        1 atgatctttg atgtgcacct taaaatgctc agtcagttcg cggattctct ccgtcagaag
       61 tgcaatctga acttccgggg atcctgtatc cttatcgtgt tgtttgtggt ccaggataac
      121 tttttccttg accgcttttg tgagagccac agagactcac ctccttcata aagaaccaca
      181 gtgttctgaa acattggaaa accctgggaa agaaccaaac attcaaacga ccccatagca
      241 acttaggact ggacaatcct gtcccgaatc gcgtcagaag gggatgcacc gtttggggag
      301 tggtctgcct aaacgtccaa tatgcgaaga ccacaagact tggattctct tttccagcct
      361 tttccttctt tatctaactt ggtagtctgc ataaatgagc cagacttgtc aactgcaatc
      421 agtttcaata tagtacacat ctggctgata tgccgagagg agagcaatat gagtatccgc
      481 aaaagagcct tctggattgt cgggtgtctt gtcatattcc cgctgtggtg tggggcggtg
      541 gaggatttct ctttcatcca cgtctccgac gatcactttc cacgcgacat cagtgccgaa
      601 acctatacgg agatggccaa gagaatcaca ccagtccaac tggctcccta tgggatcaca
      661 gcgccaaccc cttcctttct cttcaacacg ggtgatctga cggaatttgg tggtggaagg
      721 ggtgtctaca aggacttggt gaggtatctc gagaaccttg gaattccctt ctatcatgtt
      781 gccggcaacc acgacaacac atggcactct atgagacctg caatcaaaca acgctacgga
      841 gcgccgtttt attctttcga tcagcatggt tgccatttta tcggcctgga aagcgccgga
      901 atccatgaac ctctgcctgc ccttggccgt gagatgctgc tatttctgga gaatgatctt
      961 cgaaaagtct ctccagacac cccacttttc atctttattc accacaaacc ggacggttca
     1021 gagttcgcca gcagactgga acgagaccga attgtggatc tgttgagagc ttacaaatcg
     1081 gttctcttcc taacgggtca cggtcactct gcagcctcga ccccatttga cgatatggac
     1141 tgcacccaag gtggctccac atacggtccc aatgaccgag gctgggtgat cgccaccgtc
     1201 gtcaaagacc atctctacgt tgccttcgta ccgtggggtg agacacaggc gacacgccca
     1261 ctgctggagc gaccactctt cggtaagccg ccctaccttc gcattgatat tgaccatcct
     1321 cgcgcaagcc agaccttcac aaaatcactt cctctgaagg tctcgtggac gggaaaggat
     1381 atacaggtcg cctctgcaac atatcaaata gatgatggag atgaagtcgc catcactgtg
     1441 gctgagggag cctgccgggc ggagattgac atcagttccc tgctcaacgg agcccactac
     1501 cttcgcttca atgccaagga ttcagccgcc aagtcttacc atcgaaccgt ggtcttttac
     1561 aaggaaacgt cagacgcccc tcgggcgctc tggagaacaa cactcctggg ttccagtaag
     1621 tgtaccccca ccctgagcaa tggaatgctg tatgtcggcg caaacgacgg cttcctctac
     1681 gctctcgatg cccgaaacgg gaagatacag tggaagtttg aaacgggtct ggaggtcata
     1741 gcaaagcctc ttctcctcaa agacagtatc ttctttggtt ctgctgatgg gaacttctat
     1801 gccgtttcca gcaagggaaa acttctctgg aagacgcctt ccgagtcagg agatggcttc
     1861 tacagctggc ctgcaacaga cggcaagaac attttctcag gctgcgatga cggctacatc
     1921 tactgcttcg atgcggcctc ggggaacaag aactgggtgt tcaaagacac gggctatgca
     1981 gttgaatcag cggtctgctt cgctgacgat gtcgtctacg ccggatcgtg ggatcgttac
     2041 atctatgctc tcactccgat caacggtgcc ctcaagtgga agactctcgg ctccaaatca
     2101 agcacagtaa caactggtgc aagtcgctat tacgctccgg cggacaatgg ccccttggcc
     2161 gtctccgaga agatgttctg caccgaccgt ggctacgagc tgggatacta cgacgccaaa
     2221 gatggtcgcc tgttcaaaac aattgagaat gtttcggcca ttgcgctgag tgaggatcag
     2281 aaatccctct atctcagagg caacacgaac gggaa
//
LOCUS       DAOQUC010000618         2310 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_57560_length_2310_cov_3.686475, whole genome shotgun sequence.
ACCESSION   DAOQUC010000618 DAOQUC010000000
VERSION     DAOQUC010000618.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2310
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57560_length_2310_cov_3.686475"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2310
                     /locus_tag="PKH07_10740"
     CDS             <1..>2310
                     /locus_tag="PKH07_10740"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HNT35461.1"
                     /translation="LEVYSALIEAGFAVHGIPTFIDRRRPITYHPLVELVRSAVRIIS
                     RDWPSEEVFHYLKTDLIAVDPAAHEPQSNSPESPEMLSREAVDELENYVLAHGIRGSR
                     WRDDAPWDYRRQYSLGEQTSRDELEVEKEKVDVERINLHRACATAALKRFEQTVRNAI
                     NDRLVSVRTITEALYALLEDLRVPQTLERWAQELESLATSPSSLKNTRELASEHRQVW
                     NIIVELFDDLVETLGDKQYTLTDYADILEAGLGALSLGLTPPKVDQVVVGSADRSRQP
                     DLRAAFVLSLTESDFPLVRSEDAIFTDDERSRLKSLGIPLMPSTGLQLQGEKYLGYIA
                     FTRAREYLWLSYSAADLEGKKKIPSPFISQILRCFPKLSVTRKEDDEQDLKLRITSAV
                     RLAETLAGHLRPSPKPQNSAFWIALYNEAVQVPEIRDKMRHVLSSLIYRNAPSLNFDL
                     VKEHFGDRLLGSVSALETYAACPFQHFSRYTLKLTPRDKFQIEPSDLGTLYHAALRSV
                     VSQIPGQKISTLTQQEIDDMLTKTVASLVVELKGEILLSSSRNQHITSAVNRILRNLL
                     RALRQADELSAFVPLATEIEFGKSPLPALRIDLGNSREMLLRGRIDRVDGAESETGLA
                     ARVLDYKMNAHKLELCDIMSGLDLQLIAYLLVLKKCGHLLSKGQPLEPVGAFYLPIQR
                     KIKDTKNPKDEPAPDEKEYFASYKARGVFAAPYYNLLERSVQKEATFASFFVKTDGTL
                     GNLIKTDPVESEMLTRIMEETEKKLSFLGN"
BASE COUNT          559 a          608 c          611 g          532 t
ORIGIN      
        1 ttggaggttt actcggcact gatagaggcg gggttcgcgg ttcatggcat tcccaccttc
       61 attgatcgtc ggcgaccgat cacctatcac cctttggttg aactggtacg ctcagcggtt
      121 cggatcatct cacgcgactg gccttcagaa gaggttttcc actatctcaa aacggatctc
      181 atcgctgttg atcccgcagc acacgaacca cagtccaact ctcccgagtc accggagatg
      241 ctgtcccgcg aggcagttga tgaactggag aattatgtcc ttgcgcatgg gattcgcggt
      301 tcgcgatggc gtgatgatgc cccatgggac taccgaaggc agtacagtct gggcgaacag
      361 acgagccgag atgagttgga agttgaaaag gagaaggttg atgtggagcg gatcaacctg
      421 caccgagctt gtgctaccgc agcactgaag cgtttcgagc agacggttcg caatgcgatc
      481 aatgaccgtc ttgtgtcggt gcgcacgatt acagaagcgc tctatgcgct gcttgaggat
      541 ttgagagttc cccagacatt ggagcgatgg gcgcaggagt tggagtcgtt ggcgacgtcg
      601 ccctcgtctc tcaagaacac gcgcgaacta gcaagcgagc acaggcaagt ctggaacatc
      661 attgtggaat tgtttgacga tcttgtcgag actttgggtg acaaacaata cacgctgaca
      721 gactatgcgg acatccttga agcagggctg ggagcgctga gtctcggact gacgccaccg
      781 aaagtagatc aggtcgtcgt cggttcggca gatcgttcgc gacagccgga tctgagggca
      841 gcctttgtct tgagccttac ggagtctgat ttcccactgg ttcgaagtga agacgccatt
      901 ttcaccgacg acgagcgttc cagactcaag tcgcttggaa tacccttgat gccttccacc
      961 ggcttgcagt tgcagggtga gaagtacctt ggttacattg cattcactcg cgcccgagag
     1021 tacttgtggc tcagctattc tgctgccgat ctggaaggga agaagaagat tccttcaccc
     1081 ttcatcagtc agatactccg atgcttcccc aaactttcgg tcaccagaaa agaagatgac
     1141 gagcaagatc tgaagctgcg tatcaccagc gctgtgcgtc ttgcagagac actcgcagga
     1201 catcttcggc cttcaccaaa gccgcagaac tccgccttct ggattgctct ctacaacgaa
     1261 gcggtgcagg ttccagagat cagagacaag atgagacacg tactctccag cctcatctat
     1321 cgcaacgctc cttctctcaa tttcgatctt gtcaaggaac attttggtga ccggcttctt
     1381 ggcagcgtct ccgcgctgga gacatacgca gcatgcccat tccagcattt ctcccgatac
     1441 acactaaaac tgacccctcg tgacaagttc cagatcgagc cctctgacct gggcacctta
     1501 tatcatgccg cgctcaggag tgtcgtcagt cagattcctg ggcaaaagat cagcacactg
     1561 actcagcaag agatagatga catgctgacc aagaccgttg catcgcttgt cgtcgagctc
     1621 aagggagaga tacttctgag cagcagtcgc aaccagcata tcacctctgc ggtaaaccga
     1681 attctccgaa atcttctgag ggctttgaga caggcagatg aactttccgc gtttgttcct
     1741 ctggcgacgg agatcgagtt cggaaagtca ccgttgcccg ctttgcggat agacctgggc
     1801 aactctcgcg agatgctcct gagaggccgg attgatcgag ttgatggggc agagtcggag
     1861 acgggacttg ctgcacgtgt tctcgactac aaaatgaatg cacacaaact cgagctatgt
     1921 gacatcatga gtgggcttga tcttcagctg atcgcctatc ttcttgtgct caagaagtgt
     1981 ggccatctcc tctcaaaagg acagccatta gaacctgttg gcgcattcta ccttcccatt
     2041 cagcgcaaaa taaaggacac aaaaaaccct aaagatgagc cggctcccga tgagaaggaa
     2101 tactttgcat cttacaaggc gagaggcgtc ttcgccgcgc cgtactacaa tttacttgag
     2161 cgaagcgttc agaaggaagc cacctttgct tctttctttg tcaagacaga tggtaccttg
     2221 gggaatctca tcaaaacgga tccggtggaa tcagagatgc tgacaaggat catggaagag
     2281 accgagaaga aactctcctt tcttggaaac
//
LOCUS       DAOQUC010000619         2305 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_57758_length_2305_cov_4.119556, whole genome shotgun sequence.
ACCESSION   DAOQUC010000619 DAOQUC010000000
VERSION     DAOQUC010000619.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2305
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57758_length_2305_cov_4.119556"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="PKH07_10745"
     CDS             complement(<1..181)
                     /locus_tag="PKH07_10745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35462.1"
                     /translation="MRNRRILLALGVVAVGIVFYFAFSFVRSGRQIDVTLAEAQQALQ
                     EKRWEEAANGFQQVLR"
     gene            complement(541..1191)
                     /locus_tag="PKH07_10750"
     CDS             complement(541..1191)
                     /locus_tag="PKH07_10750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35463.1"
                     /translation="MAKQVEQIDYDALGLHDPAGRGSAHYYHPSHERLPPKYTFGCLG
                     PLCFLAVVLYVLYQVFCSKPYNPAERYQQTVTDSIRYYLKTTQDYLAGGRGVYAKDVM
                     FCVTKDDDWWFNHNYRAIAADKEAAMGTLSEGERKTDPAEMAMHVLLRSGPTDPAAEI
                     KVIDPRSPLATVEVTQNYTAPEPGQTGSSRTYRVELVLEHGLWKVNRFAGARDKFD"
     gene            1421..>2305
                     /locus_tag="PKH07_10755"
     CDS             1421..>2305
                     /locus_tag="PKH07_10755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020887168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HNT35464.1"
                     /translation="MTYEQSTVIVIVLATMGMFIWGRWRHDMVAAGSLLVCVFVGIIP
                     AADAFSGFSHPALMTVACVLVLSHGLQLSGAVDGLAQRVLPKSSSPTVSILALVTLAA
                     LLSSFMNNVGALALLMPLGIRVAQKHGIPAGKVLMPLSFASILGGTTTLIGTPSNLIV
                     SDFRAGTGQGAFGMFDFTPVGLVVAVVGVVFIAFVGWRLVPSRKRTDTGKFDTASYLT
                     EVRVISGSKALGKTVREVEQMLDQADAQIVGMVRNDFRVSAPHPGRIIREDDILIIET
                     EPSSLALALSHVGLHLEES"
BASE COUNT          494 a          620 c          605 g          586 t
ORIGIN      
        1 cacgaagcac ctgctggaag ccgttggccg cctcttccca tcgcttttcc tgaagcgcct
       61 gttgagcttc ggcaagggtg acgtcaattt ggcgtcccga acgcacgaaa gagaatgcaa
      121 agtagaacac gatgccaact gcaacaaccc cgagcgcaag aaggattcga cgatttctca
      181 tggttccgct tttgcctctg atagtcaggg atcagatgct attgctctgt gcccttcgtg
      241 gcacagaata cacacttgac cctgtgtctg ttccgtgaaa tcggacagtc gcaagtcaat
      301 gtcaaaactc cacttaagac tagactttcc gaatcagcct gtcaacttgg agtttggaca
      361 ccatctgcct gaagggcagt tgcaatccca cgtttttggg aattccatcc gtcgccaaaa
      421 ggactgtcac aacatactga agtgcaaaga cttggaatgt ggacagatat ctggcgtcaa
      481 tgacttggct taggtaactc cctgactcaa acatgtgtcg agaacacccc ggacagaaga
      541 tcaatcgaac ttgtcacggg caccggcaaa acgattgacc ttccacaggc catgctccag
      601 aaccagttca acacggtaag ttctgctgct gcctgtctgt cccggttccg gtgcggtata
      661 gttctgggtg acctcaaccg tggcaagcgg ggagcgggga tcaatcacct tgatttctgc
      721 cgcagggtct gtgggtccgc ttcgcaagag gacatgcatc gccatctcgg ctggatctgt
      781 cttccgctct ccttccgaga gtgtccccat ggcggcttcc ttgtctgctg caatggctcg
      841 gtagttgtga ttgaaccacc agtcatcgtc tttggtcacg cagaacatga cgtccttggc
      901 gtaaactccc cgacctccgg caagatagtc ctgcgtcgtc ttcaagtaat aacgaatgga
      961 gtctgttaca gtctgttgat agcgttctgc agggttatag ggcttggaac agaacacctg
     1021 atacaaaacg tagagcacta cggctagaaa gcacaacgga cccagacacc caaaagtgta
     1081 cttgggtggc agacgttcgt gagaaggatg gtagtaatgc gccgacccgc gacctgcagg
     1141 gtcatggagg ccgagagcat cgtagtctat ttgttcaacc tgcttcgcca tatgcacttc
     1201 cccggggctg tttaccttat cccctatgtc ttctgattat catacgccta tattcttcag
     1261 cccgtcaacc actgtttgcg attttcggac attctgcccc tttttgacac tgggctctgc
     1321 cacatctata tttactccgc ggcaggcaaa tcattcgcac ttgatgcaaa aatgatccgt
     1381 ggcctgaccg acatcaaggc cgaaggacaa ccgttatccg atgacctacg aacaaagcac
     1441 ggtcattgtc atcgttttgg ccaccatggg tatgttcatc tgggggcgct ggcgacacga
     1501 tatggttgca gctggatcgc tccttgtgtg cgtctttgtc gggatcattc ctgcggctga
     1561 cgctttttcc ggcttttctc atcctgcgct catgactgtg gcttgtgtgc tggtgctcag
     1621 tcacgggctg cagttgtccg gagccgttga cggtctggca cagcgggtgc ttcccaagtc
     1681 ttcaagcccg accgtcagca tattggcctt ggtaactctg gcggccttgc tctcctcatt
     1741 tatgaacaat gttggtgctc ttgcgctctt gatgcctctg gggataagag ttgcccagaa
     1801 acatggaatt ccggcaggaa aagtcctgat gcctctctcg tttgcctcga ttctgggtgg
     1861 aacgacaaca ctcattggaa cgccttccaa ccttatcgtg tcggatttcc gtgcgggaac
     1921 cggacagggt gcttttggca tgtttgattt cacgccggtg ggacttgtgg tggccgtggt
     1981 gggagtggtc ttcattgcgt ttgttggctg gagactcgtc ccttcaagaa aacgcacaga
     2041 tacggggaag tttgacacag cctcttacct caccgaagtt cgggtgattt ccggcagcaa
     2101 ggcacttggg aagacggttc gggaagtgga acagatgctg gatcaggcag acgcccagat
     2161 cgttggcatg gtgcgcaatg atttccgagt ctccgctccc catccgggaa gaattattcg
     2221 agaagacgac atcctcatca ttgagacgga gccttcctct cttgctctcg cactttccca
     2281 tgtagggctt caccttgagg agagc
//
LOCUS       DAOQUC010000620         2300 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_57962_length_2300_cov_3.265033, whole genome shotgun sequence.
ACCESSION   DAOQUC010000620 DAOQUC010000000
VERSION     DAOQUC010000620.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2300
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57962_length_2300_cov_3.265033"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2300
                     /locus_tag="PKH07_10760"
     CDS             <1..>2300
                     /locus_tag="PKH07_10760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35465.1"
                     /translation="SNGFLSAITKGDQTWQWQGGGVGAGIWTYPDDSSIIYSRTKLPT
                     PTASNTSTPTFTPTQTPTGSPSSTPIGGTSWEEPTRTPTTGRQPEYAGWVDVVERRGG
                     ADATATPTVYARSESEYDGYGLLQESRSGSLSSMRTTSYDYRYETEISASWKRRFDLM
                     RVSNHVGDYTAYAYNEDGHVTKVEQYDELGEDDLRLTSSESEYDVLERVVRDFVQQDA
                     TCTPLTENRRGYIPRYSWGDETPTQPDTPTPTNTPTQAQAGDTSTPTCTPTATVTLTI
                     VPLAGSLAWTEDGEGRRTEYEYDPDYGWMTFVKASNNAGTGYVWTAFAYDPNSGQRIR
                     VTSPMNKTTAYKYRTGGNLIQVEQYGNMPSPTTTPAGTPLAVTYYEYDQNGYGHNLTA
                     VRDAENYWTLYEYDDDGRIVAEKLCAQTTGTPTPTMTKGYSYSSTGMQLLESQTDFKG
                     QRTVFEYDDVGRISKKSWLNASPTPTAEVVLEYGYTEFGEVESIEATTCGGSCSETMD
                     YEYDDLGRLIARDGLDEDLGTIEYGYDAASRQTTLAYATEWDDDDPPEVISTMEREWV
                     YDLASRVVAVKANSTPVATYAYNKASQRTQASCPGFGHTTWQYNEHNGQLEKISHSNW
                     NGGGQPTPISSMQYTYNADGLRTKVEKLEGEWTDYGYDALNRLTSEQHKSLAVTMDGE
                     MDYVHVAQGSGSLDRDGASAVSIECWFRAEDVDDLTECTLFRLGEGGMHGAVECTLDR
                     MMIARVKVGSKWCYAETGSGAFPDDE"
BASE COUNT          568 a          607 c          676 g          449 t
ORIGIN      
        1 ttccaacggc tttctcagtg ccatcaccaa gggcgatcag acttggcagt ggcagggagg
       61 tggtgtcggt gctggtattt ggacctaccc ggacgacagt agtatcatct attcccgaac
      121 gaaactaccg acacccacgg catcgaacac cagcaccccc acattcacgc caacacagac
      181 tcccactggc tctccctctt ccacgcccat aggtgggaca tcctgggaag aacccactcg
      241 aacgccgact actggcaggc agcccgaata tgctgggtgg gtggatgttg tggagcgacg
      301 tggtggcgca gacgccacag caaccccgac cgtctatgcg cgctcagagt ctgagtacga
      361 tggctacggt ttgctccagg agagccggtc tggttcactc tcgtcaatgc ggacgacatc
      421 ttatgattac cgctatgaga cggaaatcag tgccagttgg aagaggcgct ttgatctgat
      481 gcgggtgagc aaccacgtcg gagactatac tgcctacgcg tacaacgaag acggtcatgt
      541 caccaaagtc gagcaatatg atgaacttgg tgaagatgat cttcgactga cctccagtga
      601 atccgagtac gatgttctgg aacgtgtggt tcgggatttt gtgcaacagg atgcaacatg
      661 tacgccattg acagagaaca ggcgtggtta cattccacga tacagttggg gagatgaaac
      721 tccaacccaa ccggataccc caacaccgac aaacaccccg acacaggccc aggcgggaga
      781 cacctcgacg ccgacgtgca ccccaactgc caccgtcaca ttgactattg ttccgcttgc
      841 cggttcattg gcgtggacag aggacggcga aggacgtcgg acagaatatg aatacgaccc
      901 cgactatggt tggatgacct ttgtgaaagc gtccaacaac gcaggcacgg gctacgtgtg
      961 gacggcgttt gcctacgacc cgaacagcgg tcagaggatt cgagtcacgt caccgatgaa
     1021 caagacgacg gcgtacaagt accgaaccgg cgggaatctc attcaggtcg agcagtacgg
     1081 caacatgccc agcccgacaa cgacccccgc aggcacacct cttgccgtga cctactatga
     1141 atacgaccag aacggatacg ggcataatct taccgcagtc cgtgatgctg agaactactg
     1201 gacgctgtat gagtatgacg atgacggcag gatcgtcgct gagaaactct gcgctcagac
     1261 aacgggcact ccgacgccga cgatgaccaa aggatattcg tactcttcga cgggaatgca
     1321 gctgttggag agtcagacgg acttcaaagg acagcggact gtgtttgagt acgacgacgt
     1381 gggacgaata tcaaagaaat cctggctgaa cgcttctccg actccgacag ctgaagtagt
     1441 gcttgaatac ggctacacgg agtttggcga agtcgagtcc atcgaggcga cgacctgtgg
     1501 aggtagttgc tccgaaacga tggactatga gtacgatgat ctcggtcggc tcatcgctcg
     1561 ggacggtctt gacgaagacc ttggcaccat cgagtacggc tacgatgcgg cgagtcggca
     1621 gaccacgctt gcttatgcca cggaatggga tgacgatgac cctccggaag tgatctcgac
     1681 aatggaacgc gaatgggttt atgatctggc aagtcgtgtt gtggctgtca aggccaacag
     1741 cacacccgtg gcgacgtatg cctacaacaa ggcatcccaa cgcacacagg cgtcttgtcc
     1801 gggattcggg catacgactt ggcaatacaa tgaacataac ggtcagctgg agaagatttc
     1861 acactccaac tggaatggcg gtggccagcc gacgcccatc agttcgatgc agtacacgta
     1921 caacgcggac ggtctgagga caaaggtgga aaagctcgaa ggtgagtgga cggattacgg
     1981 ttacgacgct ctgaaccgac tgacgtcgga acagcacaag agtcttgccg ttacgatgga
     2041 cggtgagatg gattatgtgc atgtcgccca ggggtctggt tcccttgata gagacggcgc
     2101 atcggcggtg agcatcgagt gctggttccg tgctgaagat gtggacgacc tgaccgaatg
     2161 caccttgttc cgtcttggtg aaggtgggat gcatggagcg gtggagtgta ctctcgatcg
     2221 aatgatgatc gctcgtgtca aggttggtag caaatggtgc tatgccgaga cgggcagcgg
     2281 agcgttcccg gatgacgagc
//
LOCUS       DAOQUC010000621         2300 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_57968_length_2300_cov_2.725612, whole genome shotgun sequence.
ACCESSION   DAOQUC010000621 DAOQUC010000000
VERSION     DAOQUC010000621.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2300
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57968_length_2300_cov_2.725612"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..526
                     /locus_tag="PKH07_10765"
     CDS             <1..526
                     /locus_tag="PKH07_10765"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HNT35466.1"
                     /translation="YCVPMTFKPWGLGWASRRLRRIPGSKDLFTAAAWVVVCVLEPFA
                     ARPEVHLGALLTASIWTFTLVFNRSIAFDIKDLQGDSIVGKETIPIVLGKQATKLMLL
                     GFVLFQASILIWAASSGLVSNLGYWLLGSVAFECLYLGLYHRRIITRGILFELVVDAS
                     LIVPAVITLLWEAF"
     gene            732..1832
                     /locus_tag="PKH07_10770"
     CDS             732..1832
                     /locus_tag="PKH07_10770"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNT35467.1"
                     /translation="MLAERMSRIDSSGIRKVFDLAQHMKDPVNFSIGQPDFDVPEVVK
                     EAAIQALESGFNRYTLTAGIPELREACLAHYRSKGFSADGCIITSGVSGGILLALLAT
                     MNPGDEILVPDPYFVMYKHLVNFMGGVPIFVDTYPGFELLEERLEAAVTPKTKAIFVN
                     SPANPTGKVASEDQLRMVARFAERHSLLVISDEIYEVFNYIGENPSIATFYPNTIVLN
                     GFSKIAAMTGWRVGYALASAPIIKAMTDIQQYTFVCAPSFAQKAALAALDMDTSSYRE
                     EYRRKRDQICEGLGKHYEIVKPEGAFYVFPRVPEGKGTDEEFVKRLIENNVLCIPGSV
                     FSQRNTHLRLSYAATDETIERGIEIMCRLADE"
     gene            1879..>2300
                     /locus_tag="PKH07_10775"
     CDS             1879..>2300
                     /locus_tag="PKH07_10775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35468.1"
                     /translation="MAESSRRQLALDTIQQKPTQGIPSWMLHVMQHSHIERLADCEPG
                     RYKADPENVYIRMQQRIGTCLLDQYIPTNPLTMGDHGFEENQPGACRGADTIIVDGMF
                     IDSPEAVVEHLETCVFPEVRRQISAFDEDTRVHDILNK"
BASE COUNT          537 a          612 c          608 g          543 t
ORIGIN      
        1 ctactgcgtt ccgatgacat tcaaaccgtg gggactgggt tgggcaagcc gtcgtctgcg
       61 ccgaatccca ggctccaaag acttgttcac cgcagcggcc tgggtggtgg tgtgtgttct
      121 ggaaccgttc gcagctcggc ctgaagtgca tttgggagcc ttgctcaccg cttcgatctg
      181 gacatttacc ctggtgttca atcgatccat tgcctttgac atcaaagatc tgcagggaga
      241 cagtattgtc ggcaaggaga ccattcccat tgttctggga aaacaagcca caaaactcat
      301 gcttcttgga tttgtgctct ttcaggcatc gatcctgatc tgggcggcct cttcagggtt
      361 ggtctcaaac ttgggctact ggctcctggg ttcagtggct ttcgaatgcc tctacctcgg
      421 tctctaccat cgccgcatca tcacgcgagg catccttttc gagcttgtgg tggatgcttc
      481 actcatcgtt cctgccgtga tcacactgct ttgggaagct ttctagacag gtcagcacct
      541 tcaggcattt gggctagagc tgctccccct ggactgagtg tcactggggg cagaggagaa
      601 tggacacatg ttctgactca ttgacgggaa gaggaatttc aggatagagt atctcccgtt
      661 tgtagagttc gtgttcatct atgaacagat gtcatgccgc gaggcttttc gcggcggaaa
      721 gaggcggaag gatgctcgct gaaaggatga gccgtattga ctcatctggt ataaggaaag
      781 tttttgacct ggcacaacac atgaaagacc cggtgaactt ttcgattggt cagcccgatt
      841 ttgatgttcc ggaagtggtt aaagaggcag ccattcaggc gttggagtca ggattcaaca
      901 gatacaccct gacagctggg attcctgagc tgcgtgaagc ctgcctggca cactaccgct
      961 cgaaaggttt ctcagcggac ggatgcatta tcaccagcgg cgtttcgggt ggcattttgc
     1021 tggctctcct ggcgacgatg aatccaggag acgagatcct cgttcccgac ccctactttg
     1081 tcatgtataa gcatctggtc aatttcatgg gtggtgtgcc gatctttgtt gacacctatc
     1141 cgggttttga actgctcgaa gagcgtctgg aagccgcagt cacacccaag accaaagcca
     1201 tcttcgtcaa cagtccagcc aatccgacag gcaaggtcgc aagtgaagat cagctgcgga
     1261 tggtggcacg cttcgccgag agacacagcc ttctggtgat atcagatgag atttacgagg
     1321 tcttcaacta cattggcgaa aatccctcca ttgccacgtt ctatcccaat accatcgtgc
     1381 tgaacggttt ttcaaagatt gccgcgatga ccggttggcg cgtcggatat gcccttgctt
     1441 ccgctccgat tatcaaggcg atgaccgaca ttcagcagta cacttttgtc tgcgctccgt
     1501 catttgcgca gaaggcagca ctcgccgcgc tggatatgga cacatcctct taccgagagg
     1561 agtatcgccg aaaacgcgat caaatctgcg aaggccttgg aaagcactac gaaattgtca
     1621 agccagaagg tgcattctac gtgttcccaa gagtccccga aggcaaaggc actgacgagg
     1681 agtttgtgaa acgcctgatc gagaacaacg tgctgtgcat tccgggcagc gtcttctcac
     1741 aacgcaacac acatctccga ctctcctatg ccgcaacgga tgagacaatc gagcgtggta
     1801 tcgagataat gtgtcgcctg gcagatgaat gatttcacgg tcccgtagcg cttgtcggac
     1861 tgattgacag aagacagcat ggcagaatcc agccgcagac aacttgcact ggacacaata
     1921 caacagaaac ccactcaggg cataccgagc tggatgctgc atgttatgca gcacagccac
     1981 attgaacgtc tcgccgactg cgaaccaggc agatacaaag ccgaccccga aaatgtctac
     2041 atcagaatgc agcagcggat cgggacatgt ctgctagatc agtacatccc caccaatcct
     2101 cttaccatgg gcgaccatgg cttcgaggaa aaccagccag gtgcctgtcg tggcgccgac
     2161 actatcattg ttgacggcat gttcattgac tccccggaag ccgtagtcga gcatctcgaa
     2221 acctgtgtct ttccagaagt tcggcgacag atatctgcct ttgatgagga cacgcgtgtg
     2281 cacgacatcc tcaacaagga
//
LOCUS       DAOQUC010000622         2299 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_57984_length_2299_cov_4.090018, whole genome shotgun sequence.
ACCESSION   DAOQUC010000622 DAOQUC010000000
VERSION     DAOQUC010000622.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2299
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57984_length_2299_cov_4.090018"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            75..1454
                     /gene="radA"
                     /locus_tag="PKH07_10780"
     CDS             75..1454
                     /gene="radA"
                     /locus_tag="PKH07_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011937075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HNT35469.1"
                     /translation="MKSRVRYVCVECGHAESKWFGRCPACENWNSAMEEAFQSGSNSS
                     RSGGALPVESSPVALPAIEVNTEQRLLVGIQEVDRVLGGGLVPGSVVLIGGEPGMGKS
                     TLLLQVSCNIADCHGQVLYVTAEESTRQTKLRASRLNLNAPEVYVLAETDLGLILQEA
                     EKQKPFAVVVDSIQSVSLGDVGSAPGTVSQVRECANALVRYAKRTETPVFLVGHVTKE
                     GVLAGPRVLEHVVDSVLYMEGDRHHAYRLLRAVKNRFGSTDEIGVFEMRTDGLAEVRN
                     PSRAFLQEGRQETSGSVVTATVEGTRPLLLEIQGLCTDPVSGGAARRTTSGLEGTRAA
                     MLLAVLEKRAGLPLTRQDVYLNVVGGVRVSEPAVDLAVCLAVASSFLDCEISSQTFVF
                     GELGLAGEIRTVNQAERRVLEGRNLGFQKCVLPEGNARQLSAGTCNQIQVCAVRDLPE
                     ALEAAGIHR"
     gene            1472..>2299
                     /locus_tag="PKH07_10785"
     CDS             1472..>2299
                     /locus_tag="PKH07_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011520996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain nuclease"
                     /protein_id="HNT35470.1"
                     /translation="MKSKVQLLKTVRVMFVIFCAVSGFIICSWVDPKGGIGKQAMWGM
                     VSFIVAVAYVYLESKVEYVPPRELLVGILGLTSGLIVAQLVIGVFPVSSDDPSYRISK
                     VMLNVFLSYFGLVVALRYSDRISFSGTRWTLGGSAGGSKKILDTSVLIDGRIADIVET
                     GFVEGPLVIPNFVLRELQTVADSSDPIRRKRGRRGLDVLKRLQEGHYAVQIAEEEPGT
                     HRMDVDEKLVLLAKEINASIVTNDFNLNKVASIQDIVVLNINDLTNALRPVVLPGESM
                     "
BASE COUNT          495 a          542 c          684 g          578 t
ORIGIN      
        1 tcttatgatg aagtgctata tttgaatgag ttcgatcggt tgttcgtcaa tgtggtttgg
       61 gttcgcatta tacgatgaag agcagagtcc gttacgtttg tgtggagtgt ggtcacgccg
      121 aatcaaaatg gttcgggcgt tgccctgctt gtgagaattg gaattccgcg atggaagagg
      181 cattccaaag tggttccaat tcctcgcgca gtggtggggc attgcctgtg gaaagcagcc
      241 ctgttgctct ccctgccatc gaggtgaata cggagcagag gcttctggtg ggtattcagg
      301 aagtggatcg ggtactgggt ggcggcttag ttcctggttc ggtggtgctc atcggtggag
      361 agcctggcat gggcaagtcc actctgctgc ttcaggtatc ctgtaacatt gccgactgtc
      421 atggacaggt gctctatgtc acagccgaag agtccactcg acagaccaag cttcgtgcat
      481 ccagactcaa tctgaatgcc cccgaagtgt acgtgctggc agagaccgat ctgggactga
      541 tcctccagga agccgagaaa caaaaaccgt tcgcggttgt ggtggattcg attcagagtg
      601 tttcccttgg cgatgttggt tctgctccag gcaccgtcag ccaggttcgg gagtgcgcca
      661 atgctctggt tcgctacgcc aagcgcaccg aaacgccggt cttccttgtc ggccatgtga
      721 ccaaagaggg tgttcttgca gggcctcgag tgctcgaaca cgtggtggac tccgttctct
      781 acatggaagg ggacagacac cacgcctacc gactgctgcg tgccgtcaag aaccgattcg
      841 gctccactga cgagatcgga gtattcgaga tgcgcacaga cggattggcg gaagtccgga
      901 atccatctcg cgcatttctt caggaaggtc gtcaggaaac gtcgggctca gtggtcacag
      961 cgaccgttga gggaacaaga ccattacttc ttgaaattca gggactttgt acagatccag
     1021 tctcaggggg tgcagctcga agaaccacgt cgggattgga gggcactcgg gcggcaatgc
     1081 tgttggctgt gctcgaaaaa cgcgccggac tccccttgac aagacaggac gtttacctta
     1141 atgtcgtagg tggggtaaga gtctctgaac ctgcggtgga tctggcagtc tgcttggctg
     1201 tcgcttccag ctttttggac tgtgagattt cttcacaaac atttgttttt ggggagttag
     1261 gtttggcagg ggagatacgt acagttaatc aggcagagcg acgtgttctt gagggcagga
     1321 atctcggttt tcagaagtgt gtattgccgg agggcaatgc tcgacagctg tccgccggaa
     1381 cctgcaatca aattcaggtt tgcgctgtca gggatttgcc ggaggctctt gaggccgccg
     1441 gcattcatag atgattcacg agagagagga ggtgaaaagc aaagtgcaac ttctgaagac
     1501 agtccgtgtt atgttcgtca ttttctgcgc ggtttctgga ttcatcatct gttcctgggt
     1561 cgatcctaag ggcggtatcg gaaagcaggc catgtggggc atggtctctt tcattgtggc
     1621 agtggcctat gtgtacctgg aatctaaggt cgaatatgtt cccccccgcg agctgcttgt
     1681 gggcatcctg ggactcacca gtggtcttat cgtggcgcaa ctggtcattg gagtttttcc
     1741 ggtctcatca gacgatcctt catacagaat ttcaaaagtc atgctcaacg tgttcttgtc
     1801 ttatttcgga ctggttgtag cgcttagata ctctgaccgg atcagtttct ctggtacccg
     1861 atggactctg ggcggatcgg caggcggaag caagaagatc cttgatacaa gcgttttgat
     1921 tgatggcaga attgccgaca ttgtggagac gggttttgtc gaaggcccat tggtcatccc
     1981 gaatttcgtt ctgcgcgaac tgcagaccgt tgccgattcc tctgacccga taaggcgaaa
     2041 gaggggacgg cgcggcttgg atgtgctcaa acggcttcag gaaggtcact atgccgtcca
     2101 gatcgccgag gaagaaccag gaacacatcg gatggacgtg gacgaaaaac tggtattgct
     2161 ggccaaggag atcaacgctt ccatcgtcac caacgatttc aacctgaaca aggtggcttc
     2221 cattcaggat atcgtcgtcc tgaatatcaa cgatctgacc aatgccctca gacctgtcgt
     2281 attgccaggt gagagcatg
//
LOCUS       DAOQUC010000623         2299 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_57994_length_2299_cov_3.071747, whole genome shotgun sequence.
ACCESSION   DAOQUC010000623 DAOQUC010000000
VERSION     DAOQUC010000623.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2299
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57994_length_2299_cov_3.071747"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1737
                     /locus_tag="PKH07_10790"
     CDS             <1..1737
                     /locus_tag="PKH07_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008522347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNT35471.1"
                     /translation="ELERERGITIRSKHCTFTWRDYLMNIIDTPGHADFSGEVERVLS
                     MVDSVLLLVDANEGAMPQTRYVLMRALRLGLKPIVIINKVDRPNARPDAVLDEVFNLF
                     LELGADDKQADFPVLYGSGLDGWLVRDLGTDVRYGMDALFETIIENVEPPKADPDAPF
                     LMQVSTLAWSDYIGRIGCGKVLQGRLRQGQAINRIVTKWRKAGKKEMGWDVCGTYTEK
                     AEYLWVMRGLQRVEVDEIGAGDIVWVAGPEDINIGDTFSAVDADIEPLQPLEIEEPSV
                     SMLFLVNNSPFASEEGRVAMLPQLKERLERELRTNVALRVQDIGRPDAVKVLGRGELH
                     LAILIEEMRREGLEFCVSRPEIIPHHDEKGEVQEPMEQLIIDVPEEYQGAVIEKIARR
                     KGEMVNMHNRGTGVLRMEFEIPTRGLIGYRSEFLTDTRGLGIMASRFIGYGAWRGEVA
                     SRNRGSLISMEMGVATSYTIENLQQRATMFIEPNERIYEGQIVGENSRPDDMPCKPTK
                     KKPQTNHRASNKDNAVTLNVPKKMSLDQALEWIGVDELVEVTPKSIRLRKALLGEEER
                     KQALRRRMAAAG"
     gene            complement(1794..>2299)
                     /locus_tag="PKH07_10795"
     CDS             complement(1794..>2299)
                     /locus_tag="PKH07_10795"
                     /inference="COORDINATES: protein motif:HMM:TIGR04258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="four helix bundle suffix domain-containing
                     protein"
                     /protein_id="HNT35472.1"
                     /translation="TSKKTELKLTGVARASLEELALDYEDYLRQHDLPLWEREHPALE
                     ELRRRRPESLDEFKAWVNDPAVHRRGRPEETRHTGSTGRTGQTERNPSSESSKSSASS
                     GSTPSSVSNLSSSSIAERVANGALLLIGVACFCLDRQILALGKDFEQEGGFTERLYRI
                     RKERRDR"
BASE COUNT          512 a          594 c          686 g          507 t
ORIGIN      
        1 gaactggaaa gagagcgtgg aatcaccatt cggtcaaagc actgcacctt tacatggcgt
       61 gattacctga tgaacatcat agacactccc ggacatgctg atttctcggg ggaagttgaa
      121 cgcgttctct ccatggtgga ttcggtgctt ctgcttgttg atgccaacga aggcgccatg
      181 cctcagactc ggtacgtcct catgcgtgct ctgcgcctgg gactcaagcc catcgtgatc
      241 atcaacaagg tggatcgtcc gaatgcgaga ccggatgcgg tcttggatga ggtcttcaat
      301 ctgtttttgg aactgggggc ggacgacaaa caggcggatt tccccgttct ctatggttca
      361 ggtctggacg gctggcttgt gcgcgatctg gggacagacg ttcgttacgg gatggacgcg
      421 ctgtttgaga cgatcattga gaatgtcgag ccaccgaagg ccgatccgga cgcgccgttt
      481 ctgatgcagg tcagtacgct ggcatggagt gactacatcg gacgaatcgg gtgcggcaaa
      541 gttcttcagg gcaggttgcg ccagggacag gccatcaacc gcatcgtgac gaagtggcgc
      601 aaggcgggga aaaaggaaat gggctgggat gtttgcggaa cctataccga aaaggccgag
      661 tacctctggg tcatgcgcgg actgcaacgt gtcgaagtgg atgaaattgg cgcgggggat
      721 atcgtctggg ttgcgggtcc ggaagacatc aacatagggg ataccttctc tgcagtggat
      781 gctgatattg agcctcttca gcctttggag attgaggagc cttctgtttc aatgttgttc
      841 ctggtcaaca acagcccttt tgccagtgaa gaaggtcgtg ttgccatgct gcctcagttg
      901 aaggagcgac ttgagcgcga actgcgaacc aatgttgcgc tgcgggttca ggacattggc
      961 cgtccggatg ccgtgaaagt gctcgggcgc ggggaacttc acctggcgat actcattgag
     1021 gaaatgcgac gcgagggctt ggagttttgt gtttcgcgtc cggagatcat cccacaccat
     1081 gatgaaaagg gcgaggtgca ggagccgatg gaacagctga tcattgatgt gcccgaagag
     1141 tatcagggcg ctgtgatcga aaagattgcc cgccgcaaag gtgaaatggt caatatgcac
     1201 aacagaggaa ccggtgtgct tcgcatggaa ttcgagattc caacccgagg tttgatcggt
     1261 taccgcagtg agtttctgac ggacacccgt ggtctgggaa tcatggcatc gcgattcatc
     1321 gggtacggtg cgtggcgggg tgaagtggcg tctcgaaatc ggggctcgtt gatcagcatg
     1381 gagatgggcg ttgccacttc ctacacaata gagaatctgc agcagcgggc gaccatgttc
     1441 atcgagccga atgagcgcat ttatgaagga cagatcgtgg gggagaactc cagacctgac
     1501 gacatgccgt gcaagccgac caagaagaaa ccccagacca accatcgtgc gtcaaacaag
     1561 gacaatgcgg tgactctgaa cgtcccgaag aaaatgtcgc tggatcaggc gctcgaatgg
     1621 atcggtgttg atgaactcgt cgaggtaact cccaaatcca ttcgactccg aaaggctctc
     1681 ctgggtgaag aggagcgcaa acaggcactg agacgccgca tggccgcagc cggttagtgt
     1741 tttgcgtcgc tggatccccc cacccgaggc gacgagacta cagggttctg tgctcaacgg
     1801 tctcttcgtt cctttcggat ccgatagaga cgttcggtaa agccaccttc ctgctcgaag
     1861 tcttttccca aagccagaat ctgtcgatca agacaaaagc aagcaacccc aattaacagc
     1921 aacgccccat tggcaacgcg ctccgcgatg gaagaactgg acagattgga cacgctggac
     1981 ggagtagacc cactggacgc actcgactta ctggattcac tggacgggtt tctctctgtc
     2041 tgtccagtcc gtccagtaga tccagtgtgt ctagtttctt ccggtctccc tctccgatgc
     2101 actgccggat cattcaccca cgccttgaac tcgtccagtg attccggccg ccggcgtcgc
     2161 agttcctcaa gagcggggtg ttcccgctcc cacaacggca aatcgtgctg cctgagatag
     2221 tcttcgtagt cgagcgccaa ttcttccagg cttgcacgcg ccacgccggt caacttcagc
     2281 tcggtcttct tgcttgttg
//
LOCUS       DAOQUC010000624         2297 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_58087_length_2297_cov_2.447814, whole genome shotgun sequence.
ACCESSION   DAOQUC010000624 DAOQUC010000000
VERSION     DAOQUC010000624.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2297)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2297)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2297
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58087_length_2297_cov_2.447814"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..795
                     /locus_tag="PKH07_10800"
     CDS             <1..795
                     /locus_tag="PKH07_10800"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNT35473.1"
                     /translation="SLCEQIKSEHIAPLTFTGRYPGYALRGFYFPWVISLGGLEVLNR
                     AQNLEPGAWKDPAFVRAAEYVVDFKRKGYFQSGCIGMNHTESQMEFLVGRAAMIPCGT
                     WLHSEMANLLPSDFVMEFMLCPVFEDGKGEPSLICANLNGHEWFIGSKSKHPDLAADF
                     FRYIASPQNARRFVEQKGTLMSVVGLGEVNAPPHLLKPLEYVESAKGTWSLDYEEWYP
                     EMGTEIQNLFRDLYNNLLTPEQFVEQMEKQAEKVRSDSHIQKFKVP"
     gene            808..1734
                     /locus_tag="PKH07_10805"
     CDS             808..1734
                     /locus_tag="PKH07_10805"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNT35474.1"
                     /translation="MTKQTRSKAIGRATADLKRPLALIVLFLLPAVLLYLVFFVYPMG
                     QAIRLSLFRGSAAAERFEYVGSENIAKLLLEDASFWRALWHNVQFIFVGGTATLVLAL
                     SLAVGLTWCGRGRDFFRIVFLFPNVMSVVAVSILWSFVFNPSFGLLNGLLRLMRLEQF
                     CRAWLGEPATALWAVMLIYVWCATGFYVVLFYAGLLRIPKDYVEAAQIDGANFWQQFR
                     HISLPLLSEILKIAAVYIVINSVNIFSLVFVINEGQPSRYNDVLLTYLYQQGFQNGNF
                     GYACAIGVMILITVLVFAVAVNRLFRQEPVEL"
     gene            1731..>2297
                     /locus_tag="PKH07_10810"
     CDS             1731..>2297
                     /locus_tag="PKH07_10810"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HNT35475.1"
                     /translation="MISKTARYLITMLLSLYALVVVYPLFWMIATSLKDSWSIFAEPW
                     RLFGSVHLVNYTNAWLKGALGWKFWNSLIIDTLSLVFILLLSAMVAYVLARFRFPGNR
                     FLYYLFLLGMALPVFLGIVPLFILMKNLGLLDTRIGLATVYCAYSLSFTIFVLHGFFR
                     TLPAELAEAAVMDGCSPFGVFWKVMLPLA"
BASE COUNT          481 a          606 c          571 g          639 t
ORIGIN      
        1 tctctctgcg agcagatcaa gtcggaacat atcgctcctc ttacttttac aggtcgctat
       61 ccagggtatg cgctccgagg attttacttt ccctgggtca tcagtctggg tggcttggaa
      121 gttctgaacc gggcacagaa tcttgagccc ggagcctgga aggatcctgc ctttgtaaga
      181 gccgcagaat atgtcgtgga cttcaaacga aaaggctact ttcagtcagg ctgcatcggg
      241 atgaaccata cggaatcgca gatggaattc ttggtcggtc gagcagcaat gatcccctgc
      301 ggcacatggc ttcattcgga gatggccaat ctgctcccct cggacttcgt aatggagttc
      361 atgctgtgcc cagtctttga ggacggcaaa ggggaaccaa gtttgatctg cgccaattta
      421 aacggccatg aatggttcat cggcagcaaa tcaaagcacc ccgatctggc agcggatttc
      481 ttccgctaca tcgcctctcc acagaatgcg cggagatttg ttgaacagaa aggtactttg
      541 atgagtgtcg tggggctggg tgaggtcaat gccccgccac atctcctgaa acctctggag
      601 tatgttgaga gtgccaaagg cacctggtca cttgactacg aagaatggta tcctgagatg
      661 gggactgaga ttcagaatct ctttcgcgat ctttacaaca atctgctgac ccctgagcaa
      721 tttgtcgagc aaatggaaaa acaggcagaa aaggtacgct cggactcaca catccagaag
      781 ttcaaagtac cctgacaagg ttacgccatg acaaaacaga caagaagcaa agcgatagga
      841 agagccacag cggacttgaa aaggccactg gccttgattg ttctcttcct ccttcccgcc
      901 gtgcttttat atctcgtgtt tttcgtctat ccgatgggac aggcgatacg gctctccctc
      961 tttcggggca gcgctgcagc cgagagattt gaatacgtcg gcagtgagaa cattgccaaa
     1021 ctgcttctcg aagatgcctc cttttggagg gcactctggc acaacgttca gtttatcttt
     1081 gttggcggca cagcaaccct ggtgcttgcc ttgtcactgg ctgtcggtct gacgtggtgt
     1141 ggccgcggac gagacttctt tcgcatcgtc tttcttttcc cgaatgtgat gtccgttgtc
     1201 gctgtctcta ttctctggtc ctttgtcttt aatccatcct tcggacttct gaacggactg
     1261 ctcaggctga tgcgcctgga gcaattctgc cgtgcctggt tgggagagcc tgccaccgcg
     1321 ctgtgggcgg taatgctcat ctatgtctgg tgcgccacgg gtttctatgt cgttctgttc
     1381 tacgcaggcc ttcttcgaat cccaaaggac tacgtcgaag ccgcacagat agatggggca
     1441 aacttctggc agcaattccg tcatatttcc ctaccccttc tgagtgagat tctcaagatc
     1501 gctgccgtct acatcgtcat caactcggtg aacatcttct cgcttgtttt cgtaatcaac
     1561 gaaggccagc caagtcgtta caatgacgtg ctgctcacct acctttacca gcagggattc
     1621 cagaatggaa acttcggcta tgcctgtgcc atcggtgtga tgattctgat taccgttctg
     1681 gtctttgctg tagcagtcaa ccgcctcttt cgacaggagc ctgtggaact atgatctcca
     1741 agacagcccg ataccttatt accatgctcc tttcactcta tgcgcttgtt gtcgtctacc
     1801 cacttttctg gatgattgcg acatcactca aggattcctg gagtattttc gccgaaccct
     1861 ggagactttt cggatcggtt catctggtaa actataccaa cgcctggctc aagggggcat
     1921 tgggctggaa attctggaat tccctcatca ttgacacttt gtctcttgtg ttcattctcc
     1981 tgctctcagc gatggtggcc tatgtccttg cgcggtttcg attccctgga aacagatttc
     2041 tctattacct tttccttctc ggaatggcct tacccgtctt tctgggaatc gtcccactct
     2101 tcattttgat gaagaacctt ggactcttgg atacacgaat cggtctggca accgtctact
     2161 gcgcttattc gctgtcgttc accatttttg tgttgcacgg cttcttcaga acccttccag
     2221 ccgaactggc ggaggcagca gtcatggatg gttgctcccc gtttggggtg ttctggaaag
     2281 tgatgctgcc tttggca
//
LOCUS       DAOQUC010000625         2295 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_58163_length_2295_cov_2.330804, whole genome shotgun sequence.
ACCESSION   DAOQUC010000625 DAOQUC010000000
VERSION     DAOQUC010000625.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58163_length_2295_cov_2.330804"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1236)
                     /locus_tag="PKH07_10815"
     CDS             complement(<1..1236)
                     /locus_tag="PKH07_10815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35476.1"
                     /translation="MTQQNKPNIAESLLVSDEVRYRCIQCGDCCRENWIIPVDKETSR
                     TLIERSPEEPLRTYLGGKEAISVSASAVLIYPQRMGICVFLENEGSCYLHRQFGASGK
                     GKICREFPLLVTETPHGIYVGYSFVCRSVREGTAQPELQTVQDGGDAPALETILKTRD
                     HHYRTVGSEARLGSDSRIKLSWSEYLQVEEGLTQLLSRTGTPLGDCLGAGHAYLSLLE
                     RFIREVVVLPAGSPFTRENVVSHFVQRMKQDDYALVFRVSAKRKPSGLVKRFVTAMGL
                     SFLAQIKHRGQEHRPGRLALASRVIGEFFHRKTPNPEGMIEASDSILRRYLRHLVWRK
                     DLVAREGPLGGGSLIAAYGGLLFVYGLLMERARRLALQNTIEDAFSLAVRQVERDLIV
                     HAKLCDPQQASRLRWFLGWW"
     gene            complement(1391..>2295)
                     /locus_tag="PKH07_10820"
     CDS             complement(1391..>2295)
                     /locus_tag="PKH07_10820"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNT35477.1"
                     /translation="SYRHLLSIDMVPEVEVVIRHQMILTLCAMGNLGAARRAALTLIQ
                     THRMDQDLLMQLCDAFMDLNSINPAKEILGHIVRMREEYLPARQKFIQILNIENQIEA
                     LVPGIYAKEEEYALRYVSQLMRFGHEKVAKALLSLRGSESALIRQCVVEYYYRFGYLS
                     WGEITDFLQDSSALVRCKAAEYLTRFGPSNMAPAMARALQDESDVVRACAARFLKQHG
                     TGEMLVPLKKAIEQEEDAGTRLKIRAAITAIQRRSSSRVLSESLGKSEHLRPSMLDQV
                     SVWAFCSLLAGLVMLAGWMFFRTG"
BASE COUNT          554 a          661 c          596 g          484 t
ORIGIN      
        1 ccaccaaccc agaaaccacc tcagtcgtga tgcctgttgc ggatcacaca acttggcgtg
       61 aacgatgaga tcacgctcca cctgtcgaac cgccagagaa aaggcgtctt cgatggtgtt
      121 ctgcagcgcc agacgccggg cgcgttccat cagaagtccg tagacgaaaa gcagaccacc
      181 gtacgccgca atcagtgagc caccacccaa tggcccctca cgagcgacca gatccttgcg
      241 ccagacaagg tgccgaagat acctgcgcag aattgaatcc gaggcttcga tcataccctc
      301 aggattcggc gtcttgcggt gaaagaactc gccaataact ctgctggcaa gagccaatcg
      361 gccaggccga tgctcttggc cacggtgctt gatctgtgcc agaaatgaca accccattgc
      421 cgtgacaaac cgcttgacga gaccagacgg ttttcgcttg gccgacacgc gaaagaccag
      481 cgcatagtca tcttgtttca tgcgttggac aaaatgcgaa acaacattct cccgagtgaa
      541 tggcgatccc gcgggcagaa caacaacctc tcgaatgaaa cgctctaaga gcgacagata
      601 ggcatgtcca gcgcccaggc agtcgcccaa gggagtgccg gttcgtgaga gcaactgcgt
      661 cagcccctcc tcaacctgga ggtattcact ccatgagagt ttgatccgag agtccgaccc
      721 aagtcgcgct tctgagccga ccgtccgata gtggtgatcg cgggtcttga gaatggtctc
      781 caaagccgga gcatctccac catcctggac agtctgcaat tcaggttgtg ccgtgccttc
      841 tcggacactt cggcagacga acgagtaccc aacatagatt ccatgcgggg tttccgtaac
      901 caagagtggg aattcacgac agatctttcc cttgccggaa gctccgaact gccgatgcag
      961 ataacatgag ccctcattct caagaaagac gcaaataccc atgcgttggg gatagatcaa
     1021 cacggccgac gcgctgaccg agatcgcttc ctttcctcct agatacgtcc tgagaggctc
     1081 ctcgggagat ctctcgatga gagttcgaga ggtctctttg tccactggga tgatccagtt
     1141 ctcacgacag cagtcgccgc actgaatgca gcggtagcgg acttcgtccg aaacgagaag
     1201 cgattctgct atgttgggct tgttttgttg cgtcaatggg aactgttccc cgtcagaagt
     1261 cctgtggctt tgtgcgtgtt cgtccacaaa tctccgttca ctttcccaag aaaccaagat
     1321 cgggaggagg cgctttcttt tgtgggagag ctcctcgcaa gagtggccat agcccgcgtg
     1381 cctgcgggcc ctaacctgtt cggaagaaca tccagcccgc caacatcacc aagcccgcca
     1441 acagggagca aaaagcccag acggaaacct ggtcaagcat ggacggtcga aggtgttcag
     1501 actttcccaa agattcagac agcaccctgg atgaagaacg tctctgaatc gccgtaatgg
     1561 ctgctctgat tttcagtctc gtgccggcat cctcttcctg ctctatcgcc ttcttgaggg
     1621 ggacaagcat ctcgcctgtt ccatgctgtt tgagaaaacg cgcggcacag gcgcgcacaa
     1681 cgtctgattc atcctgcaaa gcccgtgcca tcgcaggcgc catgttggag ggtccaaatc
     1741 gggtcaggta ctcggccgct ttgcatcgaa cgagtgcgga cgagtcttgt agaaaatccg
     1801 taatctctcc ccaggagaga tagccgaacc gatagtaata ctcgaccacg cactgccgaa
     1861 taagtgccga ctcggaacct cgaagggaca gcaacgcttt ggccaccttc tcatggccaa
     1921 aacgcatcaa ctggctgaca tatcgcaaag cgtattcttc ttctttggcg tagatgccag
     1981 gcacaagagc ctctatctga ttttcgatgt tgagaatctg gatgaacttc tgacgtgcgg
     2041 gcagatactc ctcacgcata cgaacaatgt gacccagaat ctccttggcc ggattgatgg
     2101 aattcagatc catgaaggca tcgcacagtt gcatgagcaa gtcctgatcc attcgatggg
     2161 tctgaatcaa ggtcagtgca gcacgccgag cagcccccag atttcccatg gcacagagtg
     2221 tcaggatcat ctggtgtctg atgaccactt ccacttctgg aaccatgtca atggagagca
     2281 gatggcgata gcttt
//
LOCUS       DAOQUC010000626         2293 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_58224_length_2293_cov_3.188561, whole genome shotgun sequence.
ACCESSION   DAOQUC010000626 DAOQUC010000000
VERSION     DAOQUC010000626.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58224_length_2293_cov_3.188561"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            39..824
                     /locus_tag="PKH07_10825"
     CDS             39..824
                     /locus_tag="PKH07_10825"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HNT35478.1"
                     /translation="MKTVLGMPTLVELHDLQANATMASELGLDFVEMNLDCPAYLPER
                     LPAEVVRQIGEESGLAFTVHLPEKLDLAVFQSKIRRGQLDFLAEVLHWTAEAGIELVN
                     MHLNEGVYFTLPDRRLWLYEAYREDFLSSLMESFRELSQLASRNRTRLCIENLGRQNP
                     TSRQALKSLLQLQGVYLTYDVGHDAEADFCFLAFVEEHAERLAHMHLHDFDGRSSHQT
                     LFSGTVNIPRMIALAQRLNLRVVVETKTVASLRESVRRLHTSL"
     gene            complement(1018..1893)
                     /locus_tag="PKH07_10830"
     CDS             complement(1018..1893)
                     /locus_tag="PKH07_10830"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HNT35479.1"
                     /translation="MNYKLAVFTDEVSQDLAKALALAKEFNVSGVEIRSVWEHGPHLL
                     SDNEVSQIGKAVKEAGMQVACIASPMFKCELEDKEAVKTHLGILDRCLEVADRLGAKV
                     VRGFTFWKRKPVADVWDEVLSEFKPVKERMKGSKILLGIENEYSVYISKASDLKRFLD
                     TLDCPEIRGIWDPANMLFDPDGEKVYPDAYQIVRPYIVHMHLKDAVKDAGEGKPKCVP
                     IADGAAVLGPQLKALDQDGYNGYISLETHWRPTKLDEETLRKPGGKEFSESGEFASRL
                     CLENLMRILKEQGIQ"
     gene            complement(1949..>2293)
                     /locus_tag="PKH07_10835"
     CDS             complement(1949..>2293)
                     /locus_tag="PKH07_10835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009648776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HNT35480.1"
                     /translation="CVVQDCQDVQAIERTRTFSGQYHVLGGVLSPMNGVGPDELNIAG
                     LLKRLEGVNEVILATNPTVEGEATAIYLGRLLKDKGIQVSKMARGLPMGGNMEFIDEV
                     TLGEALKGRQHI"
BASE COUNT          504 a          645 c          571 g          573 t
ORIGIN      
        1 gataggcact ggcccaatat tgagggaaat tcgctgcgat gaaaacagtc ttgggtatgc
       61 ccaccttggt tgaactgcat gatcttcagg ccaatgccac catggccagt gaactcggcc
      121 tcgacttcgt tgagatgaat ctggactgtc ctgcctatct gccggaacgg ttaccggcgg
      181 aggtagttcg acagatcggt gaggaatctg ggttggcctt cactgtgcat cttcccgaga
      241 aactggatct ggctgtattt cagagcaaga ttcgccgcgg gcaattggac ttccttgccg
      301 aagttctgca ttggactgcc gaagcgggta tcgaactcgt gaacatgcac ctcaacgagg
      361 gcgtctactt tacccttccg gatcggcgtc tatggctcta cgaggcatat cgcgaggatt
      421 ttctttcttc cctcatggag tcttttcggg agctgagtca gctggcgtcc cgaaatcgca
      481 caagactttg catcgagaac cttggacgcc agaaccctac atctcgccag gcgctgaagt
      541 ctctgcttca gctgcaggga gtgtatttga cttacgacgt tggacacgac gccgaggccg
      601 atttttgctt tcttgcattt gtcgaggaac atgcagagag actggcgcat atgcacctgc
      661 acgacttcga cggtcgttca tcccatcaga cgttgttctc aggaacagtc aacattccca
      721 ggatgattgc tttggcgcaa cgcctcaatt tgcgcgtggt agtggaaacg aaaactgttg
      781 cgagtcttcg agagtccgtg cgaagactgc acacatcact ttgatgcaga gtggcgcaga
      841 tcggcttcag cctgccggag gtcgctctgt gaaggctcga tgctatcaac tgcccgacag
      901 ggagacacgt attccccttc tgttctcgct gtcggcaagc aaacttcttt cacatcgtca
      961 ggcatgcaga aaggccacaa gacactctcg gcacgcctga ataaccctcg atgaggacta
     1021 ttgaatgccc tgctctttga ggatgcgcat aaggttttcc aggcaaagcc gggaagcaaa
     1081 ctcacccgat tcggagaact ctttgcctcc tggcttgcgt agcgtttcct catccagttt
     1141 tgtcgggcgc cagtgggttt ccaatgagat gtacccgttg taaccatcct gatccaacgc
     1201 cttcaactgc ggccccagga ctgcagcgcc gtctgcaatc ggaacgcact tgggtttgcc
     1261 ctcgccggcg tctttgacag catccttcaa atgcatgtga acgatataag ggcggacgat
     1321 ctggtaggcg tcgggatata ctttctcacc gtcgggatca aagagcatat ttgccggatc
     1381 ccagattcca cgaatctcag gacagtccag cgtgtcgagg aaccgtttca gatcggacgc
     1441 tttgctgatg tagacgctgt actcgttttc aataccgaga agaatcttgg agcctttcat
     1501 ccgctctttg accggtttga attcactcag aacttcatcc cagacatcgg caacgggttt
     1561 gcgcttccag aatgtaaaac cgcgaacgac cttggctccg agtcggtctg ccacttcaag
     1621 acagcgatcc agaataccca ggtgtgtctt aacggcttcc ttgtcctcca actcgcattt
     1681 gaacatcggc gacgcgatgc aggcgacctg cattcccgct tctttgacgg cctttccgat
     1741 ctgcgagact tcattgtcac tcaacaggtg cggtccgtgt tcccagacgg aacgaatttc
     1801 cactccggac acgttgaatt ccttggcaag tgccagcgcc tttgccaagt cctgagaaac
     1861 ttcatccgta aaaacagcca gtttgtagtt catcgcacag tcctccttgc gttgttaatg
     1921 cctatcatac agaactcgtt ttcacttttc agatgtgttg gcgacccttc aaagcctctc
     1981 cgagagtcac ttcatcaatg aattccatat tgccacccat gggaagtcct ctcgccattt
     2041 tcgaaacctg gatgcccttg tccttgagaa gccgtcccag atagatcgcc gttgcttccc
     2101 cttcaaccgt gggatttgtg gcaagaatga cttcgttgac accttccaga cgtttcaaaa
     2161 gtcccgcaat gttcagctcg tcgggaccca ctccgttcat cggggacaaa acaccgccca
     2221 ggacatggta ctgaccactg aaggttcgcg tccgctcgat ggcctgaacg tcctgacagt
     2281 cctgaacaac aca
//
LOCUS       DAOQUC010000627         2291 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_58308_length_2291_cov_3.614043, whole genome shotgun sequence.
ACCESSION   DAOQUC010000627 DAOQUC010000000
VERSION     DAOQUC010000627.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2291
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58308_length_2291_cov_3.614043"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1098)
                     /locus_tag="PKH07_10840"
     CDS             complement(<1..1098)
                     /locus_tag="PKH07_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016878046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHMP kinase"
                     /protein_id="HNT35481.1"
                     /translation="MAANEMNACGEIELFVPGRLCLFGEHSDWAGGYRRIDSTVHRGF
                     CLLCGTDQGIYAKIRPHKNQIIMTSTLPDGREIGPHAIEMESSVLEETARAGGFHSYS
                     AGVAYHIQKDHPIGGVEINNFRMDLPIRKGLSSSAAINVLTARAFNRIYNLGLSIRDE
                     MEYAYLGEILTPSRCGRMDQACAFGRKPVFLTFDGDLMESEILQPQESIYVLIVDLKA
                     GKNTKKILADLHSSFLSPKKAGEDVRYALGPVNEEILQQAKRAVSDGNAREIGRLMVE
                     AQRLFDEFVLPACPEELTSPKLHRVLSYAPAQELIWGGKGVGSQGDGCAQFVARGAAE
                     RDRLAEVLKPLDVNCFPLTIHPCDNSQGQESN"
     gene            1326..>2291
                     /locus_tag="PKH07_10845"
     CDS             1326..>2291
                     /locus_tag="PKH07_10845"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNT35482.1"
                     /translation="MEELRPTHEELLRRCEAAEATLAAIRSGKTNGKQGERETWVLRT
                     TELEQRSEHIKNVLLAIRNVNQIIVAETDRQQLIEKVCAVLTETMGYFQAWVALLGGD
                     GKTVSMTAFSGFDGGFQEMRQLLERGQYPDCLSRAIASATTIVIQNPEIQCPECPLVS
                     QYGGRSGLAHRLAFDSKVYGVLAVSVPAAYAYDEEEQKFFEELANDLAFALQRMEIIQ
                     ARRDAEEQYRLALFGGDLGTWDWNIQTGEVIFNTRWAEMLGYELHEIEPHIRSWEKLV
                     HPDDMPDVMQALNAHLEGKTDTYEIEHRLLHKSGEWVWVLDKGRVF"
BASE COUNT          542 a          584 c          632 g          533 t
ORIGIN      
        1 gttcgactcc tgaccttgcg agttgtcgca gggatggatc gtcagtggaa agcaattcac
       61 atccagcggt ttgagaacct ccgcgagtcg gtcgcgttcc gccgcgccac gggcgacaaa
      121 ctgcgcacac ccatcgcctt gggagccaac acctttgcca ccccagatca actcctgtgc
      181 aggggcatag gaaagaacgc gatggagttt tggagaggtc aattcctcag gacaggcggg
      241 gaggacgaac tcgtcgaaca aacgttgtgc ttccaccatg agccttccga tttcccgtgc
      301 gttgccatcg gagacggcac gcttggcctg ttgaaggatc tcctcattga cggggcccag
      361 tgcgtaccgg acgtcttcac ccgccttttt tggcgagaga aaagaggaat gaagatccgc
      421 gaggatcttc tttgtattct tccccgcctt caagtcaacg ataagcacat agatgctttc
      481 ttgaggttgg agaatctctg attccatgag atccccatcg aatgtgagga aaacaggctt
      541 acgtccaaag gcacaggcct gatccatcct gccacatctt gaaggtgtga gaatctctcc
      601 aagataggca tactccatct cgtcccgaat cgagagacca aggttgtata ttctgttgaa
      661 tgcacgggca gtgagaacat tgatggcggc cgacgaggac agtcccttcc gaatcggaag
      721 atccatcctg aaattgttaa tctccacccc accgattgga tgatccttct ggatgtgata
      781 ggccacgccc gccgagtaac tgtggaaacc accagccctt gcggtctctt ccagaaccga
      841 ggattccatc tcaatggcat ggggaccgat ctccctgccg tcggggaggg ttgaggtcat
      901 gatgatctgg ttcttgtgag gccttatctt tgcgtagatt ccctggtcgg tgccgcagag
      961 cagacagaaa ccgcgatgta ccgtggagtc gatcctgcgg taccctcccg cccagtcaga
     1021 gtgttctcca aacagacaca gtcttccggg aacaaaaagt tcaatctcac cacacgcgtt
     1081 catttcattg gcagccacag tcacgcctct ctttcctcag gttctgctgc gtgatttgcg
     1141 tatcatagca gcaatgcggt gcccattcca tatcgaaatc ccacgcacct taacaggact
     1201 tgatttccct tgcacataaa cccgattctg tgcttgatta ttcgagcaaa aaatgggtct
     1261 atgcggatcg gttgtaagtg atttctgctg gctggtcgaa cgaagtgacg tgctcaggaa
     1321 aggctatgga agaattgaga ccaacccatg aagagttgct gcggcgttgc gaggcggcgg
     1381 aggcaactct tgctgccatt cgctctggca agaccaatgg gaaacagggg gaacgtgaga
     1441 cttgggtctt gcgtacaacc gagttggagc agcgttcgga acacatcaag aatgtccttt
     1501 tggcgatccg aaatgtgaac cagatcattg tggcggaaac agatcgtcag caactcattg
     1561 aaaaagtctg cgcggttctc accgaaacga tgggttattt ccaggcctgg gtcgcactgc
     1621 tcggaggcga cggcaagacg gtgagtatga cagccttttc gggttttgat ggtggcttcc
     1681 aggagatgag gcagctgctg gaacgtgggc agtatccgga ctgtctgtcc agagcaatcg
     1741 ccagcgccac aacgattgtc attcagaatc cagagataca atgccctgag tgtcctcttg
     1801 tttcacagta cggcgggcgt tcgggactgg cacaccgttt ggcttttgac agcaaggtat
     1861 atggtgtctt ggcggtttcc gttcccgctg cctacgccta tgatgaggaa gaacagaagt
     1921 tctttgaaga actggccaat gatctggctt ttgcgcttca aaggatggag attatccagg
     1981 cgcgacgtga cgctgaagaa cagtacagac ttgccttgtt tggcggggac ttgggaactt
     2041 gggactggaa cattcaaacc ggtgaggtca ttttcaacac acgttgggcg gaaatgctgg
     2101 gatatgagct gcatgagatc gaaccccaca ttcgttcatg ggagaaactt gtccatcccg
     2161 acgacatgcc cgatgtgatg caggcgctga atgcccactt ggaaggcaag acagacacct
     2221 acgagataga gcatcgtctc cttcacaaat caggagaatg ggtctgggtc ttggacaaag
     2281 gacgagtatt t
//
LOCUS       DAOQUC010000628         2287 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_58480_length_2287_cov_3.165771, whole genome shotgun sequence.
ACCESSION   DAOQUC010000628 DAOQUC010000000
VERSION     DAOQUC010000628.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2287)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2287)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2287
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58480_length_2287_cov_3.165771"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(414..1454)
                     /gene="argC"
                     /locus_tag="PKH07_10850"
     CDS             complement(414..1454)
                     /gene="argC"
                     /locus_tag="PKH07_10850"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: protein motif:HMM:TIGR01850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HNT35483.1"
                     /translation="MKNVKLVGATGYAGGETIRLLLSHPEVRIQSATYDGKEDAPITA
                     FYPQLRGISDLRVASIDQSADVEDGLDAVFMAVPDNVACKLAPAYLAKGVPVIDFSGD
                     FRFSDSNTHKQWYGFDVDESLLKKAIYGLPELFRKQIPGAKLIANPGCYPTCALLALA
                     PALKGGFVDTETVICDAKSGISGAGRNPKSFLHFPERNENLSAYKVASHRHIPEIEVH
                     GSAFSGKNLRLTFVPHLIPINRGMLCTIYADLLSSQTQKQMNDAYLSFYENEPFVRVL
                     PASDMPSVNTVAGTNFCDLQVHVDEHTNRLIVVSVIDNLVKGASGQAVQNLNLILGLE
                     ETTGLMTPGQYL"
     gene            1730..2272
                     /locus_tag="PKH07_10855"
     CDS             1730..2272
                     /locus_tag="PKH07_10855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35484.1"
                     /translation="MSFKYLSLGILCVVLVCGCSKPKQPAQVAAEETPTAVVQPAVEV
                     AQPTAFAKPVIRKVYDQKLEGICAPSEKELSQTDDIEGNIGVVDTSEIENWRDEYTVY
                     FGLEQETESGVFEDCLQTMAWSYANIPQECPEMRASVGDSFLGTGKKYRWKAAVAVGS
                     ETPPKLISDWQFSDGFSVGQ"
BASE COUNT          577 a          561 c          619 g          530 t
ORIGIN      
        1 aataaaagtg gttatacctg cggcaaaata catacctccg ccggtggcca aagaaaccat
       61 tgactccaaa cttatcacag tctgcaaatc cactcaactt aatatgaacc ttccagagtt
      121 cgtctggatg gcttctaact gatacaacga cataaccgac aaaacgtgtt cacgaactca
      181 aaagaattca cacctaattt ctaagcagga attgagccag ttgatctgac aggactggaa
      241 tcttttcgag tgaaaactga tctgtaccgt atctatttga aagtgaaact gttatatgga
      301 caatatggcc gagcgtgcag gggagtactc gggcagacgg cttcgctttc ctcgagattc
      361 cctactggaa gtgttccgaa acgtctgcag tctgaacgtc ttcgaacgac ggctcagaga
      421 tactgcccgg gtgtcatgag tcctgtcgtc tcttccaagc cgaggatcag gttcaggttt
      481 tgaacggcct gtcctgaagc acctttgacc agattgtcaa taaccgacac cacgatcagt
      541 ctgttggtgt gctcatccac atgaacctgc aaatcgcaga agttcgtgcc ggcaaccgtg
      601 ttgacactgg gcatatctga agccggaaga actcggacga agggctcgtt ctcatagaaa
      661 gacaggtagg catcattcat ttgtttctgt gtttgcgagg acaggagatc ggcatagatg
      721 gtgcagagca tccccctgtt gatcggtatc agatgtggca cgaatgtgag tcggagattc
      781 tttcccgaga atgccgagcc atgaacttcg atttctggaa tatgacggtg tgaggcgacc
      841 ttataggcag agagattttc attgcgttcg gggaaatgca ggaaactctt tggattgcgt
      901 cccgcgccgg agataccaga cttggcgtcg cagatgacgg tctccgtatc aacaaagcca
      961 cccttgagtg ccggagcgag agccaggagt gcacacgttg gataacagcc tgggttggcg
     1021 atgagttttg caccgggaat ctgctttcgg aacaactccg gaagaccata gatggctttc
     1081 ttcagaaggc tttcgtccac atcgaagccg taccattgtt tgtgggtgtt ggaatccgag
     1141 aaacgaaagt cacccgagaa gtcaatgacg ggcactccct ttgccagata agcgggagcc
     1201 agtttgcagg caacgttgtc cggaacagcc ataaagacag cgtccagccc gtcctcgacg
     1261 tctgcagact ggtcaatcga ggcaacccgc aagtcggata tccctcgcaa ctgtggataa
     1321 aaagccgtga ttggagcgtc ttccttgcca tcataggtcg ccgactgaat cctgacttca
     1381 gggtggctca agagcagccg aatcgtctcg ccacccgcgt aacctgtcgc tccaaccaac
     1441 ttcacattct tcatcgtcgt gtcttctcct tgagatgcct tgaaagggaa atgcctcttc
     1501 aagagtatgg caatctgcca cagcattttt gggatgggtc aagacccatc acacacggtg
     1561 ttcggaatct agcacagacg aggtgggaag tgtcaagcat cgatcgttga ggatgtgcga
     1621 ctcggttgac acgaagtaac attctgctct atgacattgt ccggtgatca gaagccaaga
     1681 accccgccca attgcggcgg gggcatggaa tcacagatgg aggattaggg tgagtttcaa
     1741 atatctgtct ttgggcattc tttgtgtcgt tctagtttgt ggctgttcca agccgaagca
     1801 gcctgcccag gtggctgcag aagaaacccc aaccgcagtt gttcaacccg cggttgaggt
     1861 ggcacagcca accgctttcg ccaagccggt tatccgaaag gtatatgatc aaaaacttga
     1921 gggaatatgc gctcccagcg agaaggaact cagccagacc gacgacattg aaggcaacat
     1981 cggtgtggta gacacgagtg agattgaaaa ctggcgagac gagtacaccg tctattttgg
     2041 gcttgaacaa gaaacggaat caggcgtgtt tgaggactgc ctgcagacga tggcatggtc
     2101 gtatgccaac attcctcagg agtgcccgga gatgcgagct tcagtcggcg attccttcct
     2161 cgggacgggg aagaagtacc gttggaaggc agctgttgcg gttggctccg agacaccccc
     2221 gaaactgatt agcgattggc agttcagtga cggattctcg gttgggcagt gatccttgct
     2281 ccggaag
//
LOCUS       DAOQUC010000629         2286 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_58525_length_2286_cov_3.087405, whole genome shotgun sequence.
ACCESSION   DAOQUC010000629 DAOQUC010000000
VERSION     DAOQUC010000629.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2286
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58525_length_2286_cov_3.087405"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            90..1208
                     /locus_tag="PKH07_10860"
     CDS             90..1208
                     /locus_tag="PKH07_10860"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNT35485.1"
                     /translation="MMNGADESKRLRVLVVDDEVEILKLYERILCPDRIYRQEESTVG
                     SIQSAASIQRPQLQSSSVRFEVTLCNNGLDAVQQIQQSIDSDSAFALAFIDVRMPDGP
                     DGVWVAEQIRALDPYINIIMMTGYADVDAMDIADHVPPADKLLYLQKPINPQEILQFT
                     MALGSKWNVEWQLRRICSELRTRVEERTAELVQVNEELNEANRILQRLTITDDLTKLY
                     NKRYFNKEIAEWVEYSKRHGIPLSLLMFDIDRFKQYNDTYGHQAGDDVLEQIGMLVKH
                     SLRRQGDTPFSGIESGCRFGGEEFAIVLPRTDLKGAVAVAGRLREFVENDTKVTISVG
                     VAQLGRDEVTDEESLIRKADINLYKAKELGRNRVYWDE"
     gene            1374..>2286
                     /locus_tag="PKH07_10865"
     CDS             1374..>2286
                     /locus_tag="PKH07_10865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35486.1"
                     /translation="MPDLPNRRIRILAVDDDPRMLDLYRHVFRTSVTTQRHDEIVSLD
                     LPVPEGDDSLAETFELATASDCESALQVIRASLGEASPVAMAFIEMTLKSDEDGVQIA
                     ERIRELDPAVNLVMMTAREQPNLRDLGWRVPPPDKLLSVRKPVESEEILHLASALSAK
                     WNIERQLRRICAELETRIQEGTAELSELNDQLKVKIQEQRLIEDVLRKNEQFLESILD
                     SIQDGITVLDKDMNVLRVNRTIQKWYPQEFPFVGKKCYSTYHGLSEPCSDCPALEAIR
                     KGDIHMEVVCSTDLEGNKRWREVYIFPI"
BASE COUNT          557 a          557 c          683 g          489 t
ORIGIN      
        1 cgaatccggg catcggggga tattcttggc tcttgcgtag cttagggaat cagatggagt
       61 cggtttccag gagattcaag aggtcaaaca tgatgaatgg tgcagatgag tccaagcgtc
      121 tccgggtctt ggtggttgat gatgaagtcg agatcctcaa gctctacgaa cgaatcctct
      181 gtccggacag gatttaccgt caggaagagt ccacggtcgg cagcattcag agcgctgcca
      241 gcattcagag acctcagttg cagagttcgt ccgtgcgatt cgaagtgacc ctctgcaata
      301 acggcttgga cgctgtgcaa cagatacagc agtccattga ttcggacagc gcgtttgccc
      361 tggcgttcat tgatgtcagg atgccggacg gccccgacgg agtctgggtc gcggaacaga
      421 tccgggcact tgatccgtac atcaacatca tcatgatgac cggctatgcg gacgttgacg
      481 ccatggatat tgctgaccat gtcccacccg cggataagct gctgtatttg cagaaaccaa
      541 tcaaccctca ggaaattctg cagttcacca tggcgctggg ctcgaagtgg aatgttgaat
      601 ggcagttgcg taggatttgt tccgagttgc gaactcgagt tgaggagcga accgctgaac
      661 tggttcaagt caatgaagag ttgaacgagg ccaaccgcat cctccaacgc ctgacgatca
      721 cggacgatct gacgaagttg tacaacaagc gttacttcaa caaggagata gccgagtggg
      781 tagagtattc gaagcgccac ggaattccgt tgtcgctttt gatgttcgac attgaccggt
      841 tcaagcagta caatgacacc tacggccatc aggccggaga cgatgttctg gaacaaatcg
      901 gaatgctcgt caagcactca ctccgacgac agggcgacac gccattcagc ggaatcgaat
      961 ccggttgccg atttggtgga gaggagtttg cgatagtcct gccccgcact gacctcaagg
     1021 gagccgttgc cgttgcggga cgtctgcgag agtttgtcga gaacgacacc aaggtaacca
     1081 tcagcgtagg cgtggcgcaa ctgggtcgcg acgaagtcac tgatgaagag agcctgatcc
     1141 gaaaggccga catcaatctc tataaggcca aggagttggg tcgaaaccgc gtttactggg
     1201 atgaatgaag ggaacgcagg ggaatgaagt cttaccccct tgggtaagag gggccggggg
     1261 gatttatcga gtggacgctg aagtccgatt ggggaacggc agacagacct gggatgtaat
     1321 tccccagaaa tgaactcgcg caggctgcaa tcagggaacc gaagggacca cggatgccag
     1381 atcttccaaa ccgtagaatc agaatccttg cggtggatga tgacccccgg atgctggatc
     1441 tctatcgcca tgttttccga acttctgtta caacgcagcg tcatgacgag attgtctcgt
     1501 tggatcttcc cgtccccgaa ggggatgatt ctctcgcgga gacgtttgaa ctggcgaccg
     1561 cgtcggactg tgagagtgcg ctgcaagtga ttcgagcgtc actgggtgaa gcctcacccg
     1621 tggcgatggc gttcatcgag atgacgctga agtccgacga ggatggcgtt cagattgccg
     1681 agcggatccg agagttggat cctgcggtga atctggtcat gatgaccgcg cgcgagcaac
     1741 ccaacttgcg agaccttggt tggagagttc ccccgcctga caaactcctc tctgttcgaa
     1801 agccggttga atccgaggag atactgcatc tggcctcggc gctgagtgcc aaatggaata
     1861 tcgagagaca gcttcggcgg atatgcgccg agttggagac acgcatccag gaaggcacgg
     1921 cagagttgag cgaactcaac gatcaactga aggtgaagat tcaggagcag cggttgatag
     1981 aggatgttct ccgaaagaac gagcagtttc tggagagcat tcttgattcc atccaggacg
     2041 gtatcaccgt actggacaag gatatgaacg tgctgcgagt gaatcgcacg atacagaaat
     2101 ggtatccaca ggagttcccc tttgtcggaa agaaatgcta cagcacctac catgggctga
     2161 gtgaaccctg ttcggactgt cctgcgttgg aagccattcg gaaaggcgac attcacatgg
     2221 aagtggtctg ctcgacggat ctcgaaggga acaagcgctg gcgtgaggtt tatatttttc
     2281 ccatcg
//
LOCUS       DAOQUC010000630         2284 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_58596_length_2284_cov_3.268730, whole genome shotgun sequence.
ACCESSION   DAOQUC010000630 DAOQUC010000000
VERSION     DAOQUC010000630.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2284
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58596_length_2284_cov_3.268730"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            124..2115
                     /locus_tag="PKH07_10870"
     CDS             124..2115
                     /locus_tag="PKH07_10870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015905938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide transporter"
                     /protein_id="HNT35487.1"
                     /translation="MAHKIDKELEEFRDLMQVPSTFQEGFNWTSLLGAVFIALLMVPG
                     SIYMGLLAGTGIGPAAQWVTVILFIEVAKRAQRHLNRSEIFVLFFMAGAAMGMPFHGL
                     IWNQFFVRSDSAMAYGVAEYLPNWFAPAADSASYELRTFFHRDWIPAIALIVFGTFFG
                     QLASMILGYGLFRMASDFERLPFPMAPIGAQGIIALAEDVDEPSPEQSWRWRAFSIGG
                     ALGLAFGAIYLGLPTLSGALTGQPIQIFPIPFSDFTQKTQSYLPAVATGIAWDFGNLL
                     MGMVMPFFAMVGSLIGLIFTFVLNPVLYHFEILSSWTSGDDTISTLFKNNIDFYFSFQ
                     IGIAIAIAIAGFWQVFKSVRSSRQERSRTGVKGLSLAQRWRAEAPKQRGDIPLPWVII
                     CYFVVTMSYILVSGYLIHWHKGVMVILFALGFLYTPFISYVTARLEGIAGQVVEIPYI
                     REASLILSGYRGVACWFLPIPVVNYGGMTVFYRQCELTGTRFTGIWKTQIVLYPIILI
                     SSILFMNFIWSLADIPSAVYPFAQRMWELQSANACIMYSSTLGEYSIFEDAFNLTYII
                     CGAGFGVMLFAGLTSLGAPIFLVYGVVRGLGQNIPHSIIPQFIGALIGRYYFQKRLKV
                     MWRQYIPVVAAGFFCGQGLVTTVCVGITFLSKAAIQMPF"
     gene            2125..>2284
                     /locus_tag="PKH07_10875"
     CDS             2125..>2284
                     /locus_tag="PKH07_10875"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNT35488.1"
                     /translation="MTATSEVLIEIRNLNKVFNLGKTLVQALRDINLDIYKGEYLSIM
                     GPSGSGKST"
BASE COUNT          483 a          615 c          585 g          601 t
ORIGIN      
        1 ccctgccgga agaaaccatg gagatctatc ggcacagaac gttggaggcg atgggagaaa
       61 tcccagttca ggtgcccacg gctcctgaac acgaatcctc agactcggag aagaacacgg
      121 atcatggcac acaagattga caaggaactc gaagagtttc gagacctgat gcaggttccc
      181 tccacgtttc aggagggctt caactggacg tccctcctgg gagctgtgtt cattgccctg
      241 ctgatggttc caggctcaat ctacatggga ctgctggccg gaacgggaat cggacctgcc
      301 gcacagtggg ttactgtcat cctcttcatc gaggtcgcca aacgggcaca acgtcacctg
      361 aaccgctccg agatttttgt cctcttcttc atggcaggtg ctgccatggg aatgcccttt
      421 cacgggctga tctggaatca gtttttcgtc cgtagcgact cggccatggc ttacggtgtg
      481 gcggaatacc tgcccaactg gtttgcgcct gcggctgatt ccgcatccta tgagcttcga
      541 accttctttc atcgtgactg gattccggcc atcgccttga tcgttttcgg aacctttttc
      601 ggacaacttg ccagcatgat tctgggctat gggctgtttc gaatggccag tgacttcgag
      661 aggcttccct tccccatggc acccattgga gcgcaaggta tcattgcact ggccgaggat
      721 gttgacgaac caagtccaga gcagtcctgg aggtggcgcg ctttttccat tgggggtgct
      781 ttgggtctgg cgttcggagc gatctacctg ggactgccta ccctcagtgg agcgctcacg
      841 ggacagccga ttcagatctt cccgattccc ttttctgatt tcactcaaaa aacccagagt
      901 tatcttcccg ccgtcgcgac aggaattgcg tgggacttcg ggaatcttct tatgggaatg
      961 gtcatgccgt tctttgcgat ggtcggaagt ctgatcggcc tgatctttac ctttgtgctc
     1021 aatccagtcc tctaccattt tgaaatcctc agttcctgga catcgggaga tgacacgatc
     1081 tccaccttgt tcaagaacaa catagacttc tacttcagtt tccaaattgg aatcgctatc
     1141 gccatcgcca tcgctggatt ctggcaggtc ttcaagagtg ttcgttccag tcgccaggag
     1201 cgttcccgaa ctggcgtgaa gggactgagt cttgcgcaac gttggcgagc agaagcccca
     1261 aagcagcgtg gtgacatacc tcttccatgg gttatcatct gctactttgt cgtgacaatg
     1321 tcgtacattc tggtgtccgg ctatttgatt cactggcaca agggcgtcat ggttatcctg
     1381 tttgctctag gatttctgta tacccctttc atcagctatg tcactgctcg tctagagggt
     1441 attgcaggtc aggtggtcga gattccctac atccgcgagg caagcctcat cctcagcgga
     1501 tacagaggag tcgcctgttg gtttctgccc atcccagtgg tcaactacgg tggcatgact
     1561 gttttctaca gacagtgtga actgacaggt acccgattta caggcatctg gaaaacccag
     1621 atcgttctct atccaatcat cctgatcagt tccatcctgt tcatgaactt tatatggagc
     1681 ctcgccgaca ttccttcagc agtctacccg tttgcccaga ggatgtggga actacagtcg
     1741 gccaacgcct gtatcatgta ttcctcgaca ctcggagagt attccatctt cgaagacgcg
     1801 tttaacctga cctatattat ctgcggtgca gggttcggcg tcatgttgtt cgccggactc
     1861 accagtttgg gtgctccgat cttcctcgtt tacggagtcg ttcgtggact tggacagaac
     1921 attccgcact cgattatccc gcagttcatc ggagcgttga tcgggcggta ctatttccag
     1981 aaacgcctca aggtcatgtg gcgtcaatac atcccagtcg tcgcagcggg tttcttctgt
     2041 ggtcaggggc tggtgacaac agtatgcgtc ggaatcacct tcctgagcaa agcggcgatt
     2101 caaatgcctt tctaggaacg aataatgaca gcaacctctg aagtgcttat cgagatccga
     2161 aatctgaaca aggtctttaa cttgggcaag acattggtcc aagcccttcg agacatcaat
     2221 ctcgacatct acaaaggcga gtatctctcg atcatgggac cttcaggttc cggaaagagt
     2281 acgc
//
LOCUS       DAOQUC010000631         2281 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_58707_length_2281_cov_2.589847, whole genome shotgun sequence.
ACCESSION   DAOQUC010000631 DAOQUC010000000
VERSION     DAOQUC010000631.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58707_length_2281_cov_2.589847"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..825
                     /locus_tag="PKH07_10880"
     CDS             <1..825
                     /locus_tag="PKH07_10880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35489.1"
                     /translation="PAYRIGPPFALMNQITSESYQLTEHGEALRWFCREYVPSHPRSW
                     SFRDVKPEIAIVRFPDSCFGQRFAALPEKWGDTLYGSPHLKSTPETEAWFQIWNVLTH
                     GKTGRDGLSYFKATVAPGGYQRPVVKNVAQSLYSRPVHADAHRFFVPLNGVVVFDHLV
                     EYERLKDIPLIFVTGVEISAGTLDALRRRASEGAILVFWGPLARKLGFSQYSEGFFVM
                     DEGKGKIIITDDFGHRDLLLHIYMHIGHPDEIRYRFGDSEVILRKVTDNSVEVSLW"
     gene            1069..>2281
                     /locus_tag="PKH07_10885"
     CDS             1069..>2281
                     /locus_tag="PKH07_10885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HNT35490.1"
                     /translation="MATQRYSVTPHPIETLLTWVKSGEIAIPEIQRPFVWDATKVRNL
                     LDSLYQGYPVGYLIAWRNPTVRLKDGTCSSGKRILIDGQQRVTALMASLLGREVLTKD
                     YETVRIRIAFHPQEERFEVANPAIRKDVAWIEDVSEVFSPDASVTELTDDYADRNPNA
                     DRRHISRVLERLRKIINNHVGVIELADDLDIETVTEIFIRVNSAGTELSQADFAMSKI
                     AVNETYGGNLLRKAIDYFCHLAVAPDFLSTIEKNDKAFTTSEFLPQMRWLKDTNDDIY
                     DPTYTDMLRVAFTSEFGRGRLQDLVALLSGRNFETKQFEEAIAEESFGRLKKGISAFM
                     NRTHFERITMILRSAGFVSSALIGGRNAVNFAYILYLRGRAEGVPPADLERLVRRWYA
                     MSVLRGRYTGSP"
BASE COUNT          544 a          603 c          567 g          567 t
ORIGIN      
        1 cccgcctatc ggattggccc cccgtttgcc ctgatgaatc aaatcacttc tgagtcctat
       61 cagctgactg agcatggaga ggcactccga tggttctgcc gtgaatacgt tcccagccat
      121 ccgcgatcct ggtcatttcg agacgtaaag ccggaaattg ccatcgtccg atttccggat
      181 tcgtgtttcg ggcaacgatt cgcagcattg cctgagaaat ggggagacac tctctatggt
      241 tctccacacc tcaagagcac acccgaaaca gaggcatggt ttcagatctg gaatgtcctg
      301 acgcatggca agacaggtcg cgatggtctg tcctatttca aggcgactgt tgcccccggt
      361 ggataccagc gcccagtcgt caagaatgta gcccagtcac tgtactcacg tcccgtacat
      421 gcagatgctc atcggttctt cgtcccattg aacggcgtgg tcgttttcga ccatctggtc
      481 gagtatgagc gtttgaagga tatcccgctc attttcgtta cgggagtcga aatcagcgcc
      541 ggcacacttg atgcccttcg acgccgagca tcagaaggcg cgatcctcgt gttctggggt
      601 cctcttgcca ggaaacttgg tttttctcaa tacagcgaag gcttcttcgt gatggatgaa
      661 ggcaaaggga agatcatcat caccgatgac tttggacatc gggacttgct tcttcacatc
      721 tatatgcaca taggtcatcc tgatgagatc cgctaccgct tcggcgacag cgaagtcatc
      781 ctccgaaaag tgacggataa ttcggtcgag gtcagtctct ggtaacaccc caaccagtga
      841 tgcttatccg tctcgtaatt gtcgaataag ttcatcactc actccttcag agtgcctgta
      901 ccattgtcgt caatactctt cgaaaacttg tttcactaag ggtctccacc caaacgagga
      961 catagtcctc tcctgacttg cggtctcctc cactctctgc taaatgccta aaacactcat
     1021 cgaagaagcg taggtgctaa ctaccgactg attcaagagg ggaaaggcat ggcaactcaa
     1081 cggtattcag ttactcctca tcccatagag acccttctca cctgggtcaa gtctggcgag
     1141 atcgcaattc cggagatcca gagacctttt gtctgggatg cgacaaaggt tcgcaactta
     1201 ctcgactccc tctatcaggg ctatcccgtg ggctatctta tagcttggag gaatcccact
     1261 gtaagactca aggatggaac atgctcttct ggcaagcgca ttctgattga cgggcagcag
     1321 cgtgtcaccg cactcatggc atcgctgctt ggccgagaag tgctgactaa ggactatgag
     1381 accgttcgca tacgtatcgc ctttcatccg caggaagagc gcttcgaagt agcaaaccct
     1441 gccattcgaa aagacgttgc gtggatcgag gacgtgtctg aagtcttctc acccgacgcg
     1501 agtgtgactg aactgactga cgattacgcc gataggaacc ccaatgctga ccgaaggcac
     1561 atatctcgag tgctggagag actccggaag ataatcaata atcatgtcgg tgtgatagaa
     1621 ctggcagacg atctcgacat cgaaacggtc actgagatct tcatacgcgt gaactctgca
     1681 ggtacagaac tatcccaagc ggactttgca atgtcaaaga tcgcagtcaa tgagacatac
     1741 ggtggcaact tgcttcgcaa ggcgattgac tacttctgtc accttgccgt ggctcctgac
     1801 ttcctttcca ctattgaaaa gaatgacaag gctttcacga catctgagtt tctgcctcag
     1861 atgcgctggc tcaaggacac caatgatgac atctacgacc caacttacac cgatatgttg
     1921 cgtgtcgcgt ttacctctga atttggacgc ggaaggttac aggacttggt agctttgctc
     1981 tcaggacgca acttcgagac caagcagttc gaagaagcaa tagcggagga gtcttttggt
     2041 cgtctaaaga agggtatatc cgcgtttatg aataggacac acttcgaacg aatcacgatg
     2101 attcttcgct cagcgggttt tgtgtccagt gctcttattg ggggccgtaa tgcagtcaac
     2161 tttgcataca ttctctacct ccgcggaaga gcggaagggg ttcccccagc ggatctggaa
     2221 cgattggttc ggcgttggta cgctatgtct gttcttcgtg gaagatacac tggcagtccc
     2281 g
//
LOCUS       DAOQUC010000632         2277 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_58862_length_2277_cov_3.317732, whole genome shotgun sequence.
ACCESSION   DAOQUC010000632 DAOQUC010000000
VERSION     DAOQUC010000632.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2277
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58862_length_2277_cov_3.317732"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..722
                     /locus_tag="PKH07_10890"
     CDS             <1..722
                     /locus_tag="PKH07_10890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35491.1"
                     /translation="TMAVALALIFVISGCALFARKSESPNPETFSPMPNRIALCTVPV
                     LDSQRVPRERVESLFCQRLGQLFETVSLLTPTLTDTGAIPRGDIVDAAQRKFLAYQDL
                     SAEEVHALSARFGSDYLGIFTLTDFGQDWANLKKVTRVGVSFVVYEADQANEISRRSV
                     LVEEPDSLSGRTYEAASERAVRLVLKQAADDLRKYYRVSGGFERVPDPRVHAAEQEKA
                     PSNIAEWWQQRWRKIRESRER"
     gene            719..>2277
                     /locus_tag="PKH07_10895"
     CDS             719..>2277
                     /locus_tag="PKH07_10895"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HNT35492.1"
                     /translation="MKVSETPETRSKDTEVAASASISIDRVLGELSVNYPQADLNLIQ
                     QAFDLSRKAHEGQFRESGEPYVVHPVAVAQILAELRMDCVTIAAALLHDVVEDAGIED
                     RQLRHEFGDEIAFLVEGVTKVSRLVSDSSYEDEQLESFRRLLVATAKDQRTVLIKLAD
                     RLHNMRTLQYLSSDKRKLACQSTLDIYAPLAHRMGIGRVKDELEDLALKFLHPDIYRD
                     LKEKVAQKAPERQIIIEQASEVLRKTLQSEDLRFEISKRVKHYYSIYRKMVTTHRSFD
                     EIYDLLALRILTEKDSDCYLILGKVHQLWKPLDGRFKDFISVPKVNGYRSLHTTVVGP
                     RSRLMEIQIRTHDMHNVAENGIAAHWRYKRDQGTTTRVGDDTRWLGQFSQDLLETVSP
                     EEFMDSLKVDLFAEEIVVYTPNSDLIQLPRGSTIVDFAYKIHSEVGDHCRAGKVNGKF
                     VPLSYVIQTGDRVEVITKKEARPSRDWLSFAKTARARNKIRKFLMQVERGELLETGKA
                     LLTRELERLRVP"
BASE COUNT          533 a          560 c          662 g          522 t
ORIGIN      
        1 tcactatggc cgtggcgttg gcgttaattt tcgtgatctc agggtgtgcg ttgtttgcga
       61 gaaaatcgga aagtcccaat ccggaaacgt tttcaccgat gcccaatcga atcgcgcttt
      121 gtaccgttcc tgtgttggat agtcagcgag tgcccagaga acgggtggag agtcttttct
      181 gtcagaggct gggacagctg ttcgagacgg taagtttgct tacgccaaca ctcaccgaca
      241 caggggcgat tcccagaggg gatattgttg atgccgctca gaggaagttt ctggcctatc
      301 aggatctgag tgctgaggaa gtccatgctc tgtcggcacg attcggctca gattacctgg
      361 ggatattcac gctgacggac tttggacagg actgggcgaa tctcaagaaa gtgactcggg
      421 tcggagtgtc ctttgttgtc tacgaggccg atcaggcaaa tgagatttcc cgccggtcgg
      481 tgcttgttga agagccggac agtctgtcag gtcgaaccta tgaagccgcg tccgaaagag
      541 ccgttcgtct tgtactcaag caagcggcgg acgatctcag aaagtattac cgtgtgagcg
      601 gcggattcga gcgcgttccc gatcctcgtg tccacgctgc cgaacaggag aaggctccat
      661 cgaacatcgc cgaatggtgg cagcagcgct ggcgaaagat tcgggagtct agagagagat
      721 gaaagtgtcc gagacaccag aaacacgatc gaaggacacg gaggtggcag cctctgcgag
      781 catctccatt gatcgtgtgc tcggggaact ctccgtgaat tatccccaag cggacttgaa
      841 cctgatccag caggcgttcg atctgtcccg caaggcgcat gaaggacagt ttcgggagtc
      901 tggtgaaccg tatgtagtgc atcctgtggc cgtggctcaa atactggcag agctgaggat
      961 ggactgtgtg accatcgccg cggctctttt gcacgacgtg gttgaggatg ccggtattga
     1021 ggatcggcag ctgcgccatg agttcggcga tgagattgcc ttcctggttg aaggcgtgac
     1081 caaagtgagc cgactggtct ccgattcctc ttatgaagac gaacagctgg agagttttcg
     1141 ccggctgctg gtggcgacag ccaaggatca gcgaacggtg ctgatcaaac ttgctgaccg
     1201 cctgcacaac atgcgaaccc ttcagtatct ttcatcggat aagcgcaaac tggcctgcca
     1261 atccactctt gatatttacg ctcccctggc tcatcggatg ggtattggaa gggtcaaaga
     1321 tgaacttgag gatctggcgc tgaagttcct gcatcccgac atctacaggg atctgaaaga
     1381 gaaggtcgcg cagaaagcgc ctgagcggca gatcatcatt gaacaggctt cagaggtact
     1441 ccgcaaaaca ctccaatcag aggatctgcg gttcgagata tccaaacggg tcaagcatta
     1501 ttacagcatc tacaggaaaa tggtcaccac gcaccggagc tttgacgaga tctacgatct
     1561 tctggcgctt cgaattctga ctgagaaaga ttcggactgc tatctgattc ttggcaaagt
     1621 gcatcagctg tggaagccct tggacggacg tttcaaggat ttcatttccg ttcccaaagt
     1681 caatggatac cgttcgctgc acaccacggt tgtggggcct cgcagcaggt tgatggagat
     1741 ccagattcga acccatgata tgcacaatgt tgcggagaac ggtatcgccg cacactggcg
     1801 ctataaacga gaccaaggca ccacgacgag agttggtgac gatacacgat ggcttgggca
     1861 gttctctcag gatctccttg agacagtgag tcccgaggaa ttcatggact ctctcaaggt
     1921 ggatttgttc gccgaagaga tcgtggtcta tactcccaac agcgatctca ttcagcttcc
     1981 gcggggttcg accatcgttg attttgctta caagattcac tcggaagtgg gcgatcattg
     2041 ccgtgcggga aaagtcaatg gcaagtttgt tcctctcagc tatgtaatcc agaccggaga
     2101 tcgggtggaa gtcattacca agaaggaagc gcgaccttca cgggactggc tgagttttgc
     2161 caagactgcc cgtgcgcgga acaagattag aaagttcctg atgcaggtcg agcgaggtga
     2221 attgctcgag accggcaaag ccttactcac tcgtgaactg gagcgtcttc gcgttcc
//
LOCUS       DAOQUC010000633         2277 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_58880_length_2277_cov_2.368137, whole genome shotgun sequence.
ACCESSION   DAOQUC010000633 DAOQUC010000000
VERSION     DAOQUC010000633.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2277
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58880_length_2277_cov_2.368137"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1108)
                     /locus_tag="PKH07_10900"
     CDS             complement(<1..1108)
                     /locus_tag="PKH07_10900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35493.1"
                     /translation="MQGLFRKSVWVLVPFIGTTFLLGLAGAHSLEDLSTSSSLPFLLN
                     TLAGPDQPGAPGGIGVGSSLGFILNTLVGRRGNIALGESNSFILDTRGVYGKKLDGIY
                     VAVTVHGQIMEAFWTKDASTLRAPSWLNDAQTKLSSLVADINRERTSAEKGSILFVGK
                     PVDWAQATHSGIPLIASGRMLELAWSLGGLARVDRLVKGLLYQASGKQLTSAASRAAA
                     KQAATSLAGTVSRLKASYPNTPVFIDFIGHSRGGAVSSQTIRDFYSLGSFPGVGVSFT
                     VLDGIDPTDGGLRTLAGNMLGDPEIEKGKEKRISNFYADRAMLTLDYHRIWWGNLLLW
                     VNVHFGLELLDATLIVNGAPKGRSRPELSPERDLL"
     gene            complement(1193..>2277)
                     /locus_tag="PKH07_10905"
     CDS             complement(1193..>2277)
                     /locus_tag="PKH07_10905"
                     /inference="COORDINATES: protein motif:HMM:NF012307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="reverse transcriptase/maturase family protein"
                     /protein_id="HNT35494.1"
                     /translation="TELLEGSYRPGEFRYFKICDPKERTIAESPFRDRVVHHAICLVL
                     EPLLERRYIHDSYACRPGKGAHRALARAQQYCRRNTHYLKCDVKKYFDSIDHEVLKSL
                     LRRITKDTRLLRILDLIVDHPGGSKGVPIGNLTSQHFANLYLDRLDHWLKEIGQVQDY
                     LRYMDDFICFGNSKATLHSLRSEVGSFLETHLRLQLKEEVTHVASVHQGLSFLGFRIF
                     PSVIRIKSECVTRCRRKIVLRMNQLEMGKISEAKFLDSVQSLVTHITHGNTLLGRRSW
                     LRTLSLEGENEGMVRNGSNRVIRGGSWNNDANNARCSNRNNNTPTNRNNNIGVRLLSS
                     SESENDAVYGLRRSALLDDQNRTLFR"
BASE COUNT          555 a          642 c          558 g          522 t
ORIGIN      
        1 gaagcagatc tctctcgggt gagagttcgg gtcgcgaacg ccctttcggg gcaccgttga
       61 cgataagcgt cgcatcgagc aactcaagcc cgaagtgcac attcacccac agtaggagat
      121 tcccccacca gatcctgtga tagtccagcg tcaacatcgc acgatctgcg tagaaattgc
      181 tgatacgctt ctccttgcct ttctcgattt ccggatcacc gagcatattg ccggcaagag
      241 tacgtagccc tccgtcggtt ggatctatcc cgtcaagaac ggtgaaagag accccaacac
      301 ctgggaatga acccaaagag tagaaatcac gaatcgtctg ggatgagacc gcaccccctc
      361 ggctatgacc gatgaagtct atgaaaacag gcgtatttgg atatgaagcc ttcaacctgc
      421 tcactgtacc cgcaagagac gtcgctgcct gtttggctgc tgcccttgag gcagcggatg
      481 tcagttgttt gccgcttgcc tggtaaagaa gcccctttac cagccggtct actctggcaa
      541 gaccccccag cgaccaagcc aactccagca tgcgtcccga ggcaatcaac ggaatgccag
      601 aatgcgtggc ttgtgcccaa tccactggct taccgacgaa aaggatgctg cccttctccg
      661 cagacgtccg ctcccggttt atatccgcca caagggagga cagtttcgtc tgtgcgtcat
      721 tgagccacga gggggcccgc aaagtcgagg catcctttgt ccagaaggct tccatgatct
      781 gaccatgtac agtcaccgca acatagatgc catcgagttt tttcccatag acgccacgcg
      841 tatccagaat gaaagaattg ctttccccaa gagcgatatt gcctctgcgt cccaccaggg
      901 tgttcagaat gaagccaagc gaactcccca caccaattcc acccggagcc cccggttggt
      961 cgggtccggc aagtgtgttc agtagaaaag gcaatgaaga cgacgtgctc aggtcttcta
     1021 acgaatgggc acccgccaac cccaacagaa acgttgtccc aatgaaagga accaagaccc
     1081 acacgctttt cctaaagagt ccttgcatta ttcagacctc caatattgcc agggcggagc
     1141 gttcggctga atcctaccag ccccgccgcg gcttctgctg tttgtccggc tgttaccgga
     1201 acagggtacg gttctggtca tcaagcaatg cactgcgacg cagtccatag acagcatcgt
     1261 tctcgctttc tgatgagctg aggagacgga ccccgatgtt gttgttccta ttcgtgggcg
     1321 tgttgttgtt gcgattcgag caacgcgcgt tgttcgcatc gttgttccaa ctgcccccgc
     1381 ggatcacgcg gttggagccg ttccgaacca tgccctcatt ttcaccctcc agactgagtg
     1441 tacgcaacca ggagcggcga cctaacaaag tattgccatg agtaatgtga gtcacgaggc
     1501 tctgcacaga atcaagaaac ttcgcctcac taatctttcc catctctaac tgattcatcc
     1561 tcagcacgat cttcctgcga catcgggtca cgcactcgct cttgatacga atgacagacg
     1621 gaaagattcg aaacccaaga aaactcagac cttgatgcac cgaagcgacg tgggtgacct
     1681 cttccttgag ttgaagtctg aggtgcgttt cgagaaaaga accaacctca gaacgtaaag
     1741 aatgaagggt agccttcgag ttgccaaaac agataaaatc gtccatatag cgaaggtaat
     1801 cttgaacttg acctatctct ttcaaccaat gatccagtcg atcaagatag aggttggcaa
     1861 aatgctggct ggtcaggttg ccaatcggca cgcctttgct tccgccggga tgatctacga
     1921 ttagatccag tatcctcagc agacgcgtgt cctttgtaat gcgacgcagt agcgacttga
     1981 gtacttcatg atcaatcgag tcaaaatact tcttcacgtc acatttcagg taatgcgtat
     2041 tccgacgaca gtattgctgt gcacgagcca aagccctgtg ggctcctttg ccaggacggc
     2101 acgcataact gtcatgaatg tatcggcgtt caagaagtgg ctccaagacc agacatattg
     2161 cgtggtgaac gactctgtcc ctgaagggtg attctgcgat cgttctttcc ttgggatcac
     2221 aaattttgaa gtaccgaaac tctccggggc gataactccc ttccaggagt tctgtct
//
LOCUS       DAOQUC010000634         2274 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_59010_length_2274_cov_2.656151, whole genome shotgun sequence.
ACCESSION   DAOQUC010000634 DAOQUC010000000
VERSION     DAOQUC010000634.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2274
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59010_length_2274_cov_2.656151"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1497)
                     /locus_tag="PKH07_10910"
     CDS             complement(<1..>1497)
                     /locus_tag="PKH07_10910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35495.1"
                     /translation="ILDDKVKGHVPVKVISPGQLEIIANRVPQIRVTQPTLGSRDSVV
                     WQNDYEIEYLYLDDDTTTIELFHDRDRNVGGEVLIASLTGQASPRDATDLVTSGISDR
                     FTWDISDVTRVPAGNYYIKARIADGVNSTTVYSEDYIRVNKRPTFQFVTDDPAAPIVQ
                     GETFRFRWDAADPDSPGAQITLILRSNTGKGTVSLVEPFAVSSGLWELALQTATRQPD
                     DYSIIGSINDGVNPPVTDEIRFILQKNEPPSIAVLRPDVQDSADIIYSATFLVRWTDA
                     DPESSAAISNATVSLYMDTNNFGWDGTLLSGSYITGNVIYPSSGIPANIDNDGTEDVF
                     VWDLSQVPSGRYFVYAKILDGVNAPVYSYSSAAVTINKRPTLTFTSPRPGGMDSVAVG
                     ANYEIAWEAYDMDSQATLWLAVDDDNDPYDEDGIGRILTRDLFEKDGHDGYNWNTSGF
                     QPGIYYVVARLDDGGGNPVIVYSDGRVLVTSAGELTMTFLRPDANDSLG"
     assembly_gap    1498..1507
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1508..>2274)
                     /locus_tag="PKH07_10915"
     CDS             complement(<1508..>2274)
                     /locus_tag="PKH07_10915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35496.1"
                     /translation="VPIPATDLGTDLGSDFFVTFKTSGSLTKRSRFSIEVKKIVYGHG
                     LGERSLRTRPVSGSQFNQRPYIELLNPLTGDKENRLYSIRWSDFDPDSDATIRLYYVS
                     RADVEASSDINDWTKQTLALNISEDDETDIFHWDVSRLGVGEWYILALIRDEKSSMYA
                     VSPGYLKTANQPPFLEFIEPDGIDDRIIQGEPYSLSWVDSDPENNAYIDLYLDMDGNA
                     ATDGAFDITVATGLREDDEADFYLLDTSKIPGGSYFAV"
BASE COUNT          516 a          626 c          609 g          513 t
ORIGIN      
        1 ccgaggctgt cgtttgcatc cggcctgagg aatgtcatgg tcagttcacc cgccgacgtg
       61 accagcaccc gaccgtcaga gtagacaatg acagggttgc caccaccgtc atccagacgc
      121 gcaacaacgt agtagattcc aggctggaat ccggatgtgt tccagttgta gccgtcgtgg
      181 ccatccttct caaacagatc ccgggtaaga atccttccaa ttccgtcctc atcgtacggg
      241 tcgttatcgt catccactgc aagccagagc gtggcctgtg agtccatgtc ataggcttcc
      301 caggcaatct catagtttgc cccaacggca accgaatcca tgccacccgg gcgtggactg
      361 gtgaaggtga gcgtcgggcg cttgttgata gtcaccgccg cgctgctata cgaatagacc
      421 ggcgcattga caccatccaa aatcttggcg tagacgaagt aacgtcccga gggaacttgg
      481 ctgagatccc agacaaaaac atcttcggtg ccatcgttgt caatgttggc tggaatgcca
      541 gacgacggat agatgacatt gccggtgatg tacgatccac tcagcagggt gccatcccaa
      601 ccgaagttgt tcgtgtccat atacaggctg accgtcgcgt tacttatcgc cgcactgctc
      661 tcgggatcgg cgtccgtcca acgcaccagg aacgtggcgg agtagatgat gtccgcagag
      721 tcctgaacat ccggtcgaag cacggcaata cttggtggct cattcttttg aaggatgaac
      781 cgaatctcat ccgtgacggg aggattgacc ccatcgttga tggaaccgat gatagagtag
      841 tcatccggtt gtcttgtggc agtctgaagc gccagttccc agagtcctga agaaaccgcg
      901 aacggctcca cgagggacac agttcccttg cctgtattgg agcgaaggat caatgtgatc
      961 tgagcccctg gcgaatcggg atccgccgca tcccagcgga agcggaatgt ctccccttgc
     1021 acgataggtg ctgccggatc atcggtcacg aactggaagg tcggacgctt gttgactcgt
     1081 atgtagtcct cactgtagac cgtcgtgcta ttcacaccat cagcaatgcg tgccttgatg
     1141 taatagttgc ctgcagggac acgagtgaca tcgctgatgt cccaggtgaa tctgtctgag
     1201 ataccgctgg tgaccagatc tgtggcgtca cgcggtgagg cttgaccggt cagtgaggcg
     1261 atgaggactt caccaccgac attcctgtct cggtcatgga acaactcgat cgtggttgta
     1321 tcatcgtcca ggtacaagta ctcgatctcg taatcgttct gccagacaac cgaatcgcgg
     1381 gagccaagag tcggctgtgt gactcggatc tgaggcactc tgttggcaat aatctccaac
     1441 tgacctggcg agataacctt gacaggaaca tgtcccttga ctttgtcatc caggatcnnn
     1501 nnnnnnnacg gcgaaatacg aaccaccagg aatcttgctg gtgtccaaca gatagaaatc
     1561 tgcttcatcg tcctcgcgca ggccggtcgc cacggtaatg tcaaaggcac cgtccgtcgc
     1621 tgcattgcca tccatgtcga gatagagatc aatgtaagcg ttgttctctg gatcagaatc
     1681 cacccaggag aggctgtacg gctcgccctg gatgatccga tcatcaatgc catccggctc
     1741 gatgaactcc aggaatggcg gctggttcgc cgtcttcaga tagcctgggc tgaccgcata
     1801 catgctgctt ttctcatcac ggatcagcgc caggatgtac cactccccaa cgccaagtcg
     1861 ggagacatcc caatggaata tgtccgtctc gtcatcttcg gagatgttca gagccaatgt
     1921 ctgtttcgtc caatcgttga tgtccgaaga ggcttccacg tctgccctgg agacatagta
     1981 cagtcgaatc gtggcatcac tgtccggatc gaaatcactc catcgaatac tgtacagacg
     2041 attctctttg tctccggtga gtggattgag caattcaatg tatggacgct gattgaactg
     2101 agagccgctc actggccgcg ttctgagaga acgctcacca agtccatgtc cgtagacaat
     2161 cttcttgact tcgatggaga atctcgaccg cttggtcaga gagcccgaag tcttaaaggt
     2221 cacaaagaag tcgctgccca gatcggtgcc gagatcggtc gccggaatcg gcac
//
LOCUS       DAOQUC010000635         2274 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_59017_length_2274_cov_2.374493, whole genome shotgun sequence.
ACCESSION   DAOQUC010000635 DAOQUC010000000
VERSION     DAOQUC010000635.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2274
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59017_length_2274_cov_2.374493"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            77..>2274
                     /locus_tag="PKH07_10920"
     CDS             77..>2274
                     /locus_tag="PKH07_10920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNT35497.1"
                     /translation="MPRKPYAFDNYPVIRSNQNGIYSYGPTHPVTWLHFGFGATFDER
                     GQVKTVADRWSWRFLASRLNLLDCFSGYVVLPSQIGPLEANAYKAKGNSVLELAKSYT
                     ETVDGVFLWYCEDKIDAIKYFGLQSVVGLANGPDSLQETSEQGEKSIPTDGIGFPTRQ
                     VWTPPSSARRSASDLWRLNESRLELLRSKDIINNSVEELHGHAEDLMLQAGELPSLST
                     REALANSAFLIERPVYLDTKTTLDDLIRAVLILLGLCVPFAFAVERLLIGATTIYRQI
                     AGFTGFFLCTFLILFFTHPAFAISATPIVIFLGFAVVVLSSLVIFIIMRKFEVELKVL
                     QGLTSTVHAADVSRFSTVMAAMSMGISTMRRRPLRTFLTATTIILLTFTILCFASFGT
                     QTGIVRLFLDASPDYSGVFVHDVNWSNFNLEVLDVLRGRWSQTTTICPRMWISPKTEQ
                     NQGPLLTHEDGSAPLALKGVLGLASEEIALRPDLQNLMGTDTRNWDGRVFITQAVSEY
                     TGLKEGDAAIVGGLRLSVGKILEGSQLATVTDMDRNSILPVDFVTMQSVIDTAKQQQQ
                     QQRKADTLAREVQQNWAHLPTDSVVIVSLTNARIMGANLHAVTLYTSNNWSAAELAAD
                     LAKVLDLSISATRPEGVFRHTLGLIVQASGAKDLFFPVVLGGLVIFGTMLGSVADRER
                     EIYTFSSLGLAPPHVASLFFAEAMVYSVLGGMGGYLIAQAMMKMLSFLAGL"
BASE COUNT          491 a          647 c          573 g          563 t
ORIGIN      
        1 catgccgaat atgccgatgc ctcgcaccac catccaggcc atccacaaat tccacaccga
       61 tgtccagcct ggattcatgc cgcgcaagcc gtacgccttt gacaactacc ctgtcatccg
      121 gtcgaatcag aacgggatat actcctacgg accgacacat ccggtaacct ggctgcattt
      181 cggcttcgga gccactttcg acgagcgcgg acaggtcaaa acagttgccg atcgttggtc
      241 gtggcgattc cttgcttcgc gtctgaacct cttggactgc ttcagcggct acgtggtact
      301 cccctcgcaa atcggcccct tggaagcaaa cgcctataag gcgaaaggca acagtgtcct
      361 ggaactggca aagtcctata cggagaccgt ggacggtgtc tttctgtggt attgcgaaga
      421 caagattgat gccatcaagt attttgggct tcaaagcgta gtaggccttg caaacgggcc
      481 tgactcactc caggaaacct ccgagcaagg ggagaaatcc atcccgacag atggaatcgg
      541 tttcccaacg agacaggtat ggacacctcc atccagcgcc cgacgctcgg cttccgatct
      601 ttggcgactc aatgagtcac gtctcgaact acttcgctcc aaggacatca tcaacaactc
      661 tgttgaagag ttgcacggtc acgctgaaga tctgatgctt caggccggtg agctgccgtc
      721 tctttcgaca cgcgaagcgc ttgcaaattc tgctttcctc atcgaacgtc cggtctacct
      781 ggataccaag accaccctgg acgatctcat tcgcgccgta ctcatccttc ttgggctttg
      841 cgtcccgttc gcctttgcag tggagcgact cttgatcggt gcaacgacca tctaccgcca
      901 aatcgctgga ttcacaggtt tcttcctctg tacttttctg attcttttct ttacccatcc
      961 cgcattcgcg atttcagcaa ctcccatagt catctttctc ggctttgccg tcgtggttct
     1021 ttcatcattg gtcatattta tcatcatgcg gaagtttgag gtcgagttga aggtgctcca
     1081 agggctgacc tctacagtcc atgccgctga tgtgtcacgc ttcagcacgg ttatggccgc
     1141 catgagtatg ggcatctcca caatgagaag acgccctctg aggacgtttc tcacggcaac
     1201 aactatcatc ttgctcactt tcaccatcct gtgttttgct tcatttggaa ctcagaccgg
     1261 catcgttcgg ctgtttctgg atgcctcacc ggactactca ggagtcttcg tgcatgacgt
     1321 gaactggtcg aatttcaacc tggaagtcct ggacgttctt cgcggacgtt ggtcacagac
     1381 gacgacgatc tgccctcgga tgtggatttc ccccaagacc gaacagaacc aaggtcctct
     1441 tctgacccac gaagacggtt cggctcctct ggctctcaag ggggtgctgg gattggcttc
     1501 ggaggagatc gccctccgtc ccgacttgca gaaccttatg ggaactgaca cgcgcaactg
     1561 ggacgggaga gtcttcatca ctcaggcagt gtctgagtac actggtttga aggaaggtga
     1621 cgccgctatc gtggggggac ttcgcctttc ggtgggaaag attcttgagg gaagtcaact
     1681 ggcgaccgtc acggatatgg atcgcaacag tattctccct gttgatttcg tcacaatgca
     1741 gagcgttatt gatacagcca agcagcaaca acaacaacag cgaaaggcag atacccttgc
     1801 cagagaagtc caacagaact gggcgcactt gccaacagat tctgtagtca ttgtctccct
     1861 gaccaacgca cggatcatgg gcgccaacct acacgcggtg accctctaca cttcgaacaa
     1921 ctggtctgca gcggaactgg ctgcagatct cgccaaggtt ctcgaccttt caatcagtgc
     1981 cactcgacca gagggcgtct tccgtcacac gctgggactt atcgttcaag ccagtggtgc
     2041 aaaagatctt ttcttccctg ttgtgctcgg gggacttgtc attttcggca cgatgctggg
     2101 ctccgtggca gatcgcgaaa gagaaatata cacctttagt tcactgggtc tcgcaccacc
     2161 acatgtggcg agtttgtttt tcgctgaagc catggtgtat tccgttctgg gtggcatggg
     2221 aggctatctg atcgctcagg cgatgatgaa gatgctctcg tttcttgcag gact
//
LOCUS       DAOQUC010000636         2273 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_59027_length_2273_cov_3.899459, whole genome shotgun sequence.
ACCESSION   DAOQUC010000636 DAOQUC010000000
VERSION     DAOQUC010000636.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2273
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59027_length_2273_cov_3.899459"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..901
                     /locus_tag="PKH07_10925"
     CDS             <1..901
                     /locus_tag="PKH07_10925"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNT35498.1"
                     /translation="SPSVPSWLSRLKPDLVHIHHPYPLGFVSYILAGRSKPLVVTWHS
                     DIVRQKVLKVIFRPVQNAVLRRSNAIIVTSEPLMKQSPDLRKYQTKCLPIPLGVDVGR
                     LDSLRATAAARGSQAAMLQHSPSILFVGRLVRYKGLEVLLRAMVGIQANLFVVGEGIL
                     RGSSQRLASELGVADRVIFLGKVPEETLAGLYAGCSVFVLPSVSNNEAFGLVQLEAMA
                     ASKPVVSTNLPTGVPYVNRDEVTGYVVPPDNPEALMSALNRLLATPSLALRMGQSGRS
                     RVEQEFRLELMIERTLEVYRRCL"
     gene            971..1753
                     /locus_tag="PKH07_10930"
     CDS             971..1753
                     /locus_tag="PKH07_10930"
                     /inference="COORDINATES: protein motif:HMM:NF015297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HNT35499.1"
                     /translation="MKNPVRRALLERRITLGSWLQVNHPVSAEILAECGFDWLATDCE
                     HTDIDVGGFAQLARGMYGRGPVPFARVRENDTLAIRQMLDMGAQGVLVPLVNTPEEAL
                     KAVRAAKYPPRGIRGFCFSRMNNFGRDFEEYVKNANDEIAVIVMIESKQAVDSIDEIL
                     AVDGVDGIFIGPYDLSGSLGIPGQTSHPLILESCAKVSDACRRLGKSAGAHVVIPTPE
                     AIEKTIADGFTFIALGIDTLFLREGATAALNLAKAVNSKNSA"
     gene            1778..>2273
                     /locus_tag="PKH07_10935"
     CDS             1778..>2273
                     /locus_tag="PKH07_10935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020151242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNT35500.1"
                     /translation="MRVLVTTTSYQDTPGPHHDLLRETGYEIVAKRGPLSESEMLALV
                     GEIDGLLCGDDAITRKVLEKSLPRLKVLSKYGIGLDKIDLKAAEALGIPVCYTPGVNH
                     TTVAEHCFGLMLCLYRNIPREDALVKSGKWKRITGHELFGKTLGILGLGRIGKEVAVR
                     ALAFG"
BASE COUNT          462 a          527 c          666 g          618 t
ORIGIN      
        1 gagtccgagt gttccttcct ggttgtctcg actaaagccc gatttggttc acattcacca
       61 tccctatccg ctagggtttg tgagttacat tcttgctggc cgttcgaagc cgttggttgt
      121 tacctggcac agtgacattg ttcgtcagaa agtgctgaag gtgatctttc gacctgttca
      181 gaacgcggtg cttcgtcgat ccaatgccat tattgtgact tctgagccgt tgatgaaaca
      241 gtcgcctgat ctgaggaagt atcaaacgaa atgtctgccg attcctttgg gtgttgatgt
      301 ggggcgactg gattctcttc gcgcaacggc tgccgcgcgg ggaagtcagg ccgctatgtt
      361 gcagcattct ccttcgattc tttttgttgg tcgcttggtt cggtacaagg gactcgaagt
      421 tcttcttcgt gccatggttg ggattcaggc caatctgttt gtcgttggag aggggattct
      481 ccgaggatcg tcgcagaggc ttgcgtccga gttgggagtg gcagaccgcg tcatctttct
      541 tggcaaggtg ccagaagaga ctcttgctgg tctctatgcc gggtgcagcg tctttgtcct
      601 gccctcggtg agcaacaacg aggcctttgg actcgtccag ttggaagcca tggcagccag
      661 taaacctgtg gtcagcacga accttcctac aggtgtaccg tatgtcaaca gagacgaggt
      721 cacgggttac gttgttcctc ctgacaatcc tgaggcgctc atgagtgcgc tgaatcgttt
      781 gcttgccact ccgagtttgg cgctgcgcat gggtcagtcg ggacgttcac gggtcgaaca
      841 ggagtttcga cttgaactga tgattgagag aacgcttgag gtgtatcgcc gctgtctctg
      901 acttggatat tcttgctcag attgagccat tttggtaatg tctgtccgtt ttgtttggga
      961 ggtcttgttc atgaagaatc ctgttcgtag agcgcttctt gagcgacgca tcaccttagg
     1021 tagttggctt caagtgaatc accctgtgtc cgccgagatt cttgctgaat gtggttttga
     1081 ctggcttgcc acagactgtg agcacacaga cattgatgtc ggtggctttg cgcagttggc
     1141 gaggggtatg tatggacgtg gtccggtgcc gttcgcccgc gttcgggaaa acgacaccct
     1201 tgcgattcgt cagatgctgg atatgggagc gcagggtgtt ctggttccac tggtgaatac
     1261 tcctgaagag gcattgaagg cagttcgtgc cgccaagtat ccgccaaggg gtattcgcgg
     1321 gttttgcttc tcgaggatga acaatttcgg tcgggacttt gaggagtatg tcaagaacgc
     1381 aaatgacgag attgcggtca ttgtcatgat cgagtccaaa caggcggtag attctattga
     1441 tgagattctg gcagtagacg gtgtggatgg catttttatc gggccttacg atctgagcgg
     1501 ttccttggga attccaggtc aaacttccca tccactcatt ttggaatctt gtgcgaaagt
     1561 gtccgacgcc tgcagaagac ttgggaaatc ggcgggtgct catgttgtca ttccgacacc
     1621 tgaggcgatc gagaagacaa tcgccgatgg gtttactttc attgctctgg gaattgacac
     1681 actcttcctg agagaaggtg ccaccgcagc gttgaatctt gccaaggcag tgaactcgaa
     1741 aaattctgcc tgacagtatc atcaggggag ttgaactatg cgcgtgcttg ttacaaccac
     1801 gtcttatcag gataccccag gccctcatca cgatctcctg cgagagactg ggtacgagat
     1861 tgttgcgaag cgaggacctc tttcggaatc cgaaatgctc gctctcgttg gagaaattga
     1921 cggactgctc tgcggcgatg acgccatcac tcggaaggtg cttgagaagt cgctgccgcg
     1981 tttgaaggtg ctgagcaagt acggcatcgg gttggacaag atcgatctca aagcggccga
     2041 ggcgttaggt attcctgttt gctacactcc tggagtgaat cataccaccg ttgccgagca
     2101 ttgctttggg ctgatgctct gtttgtaccg caacatcccg cgtgaagatg ctctggtcaa
     2161 gtcaggaaaa tggaaacgca tcacaggtca tgaactcttt gggaagacct tgggaatcct
     2221 cggtctgggt cgaattggaa aagaggtcgc agttcgggcg ttggcatttg gca
//
LOCUS       DAOQUC010000637         2271 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_59114_length_2271_cov_3.753159, whole genome shotgun sequence.
ACCESSION   DAOQUC010000637 DAOQUC010000000
VERSION     DAOQUC010000637.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2271
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59114_length_2271_cov_3.753159"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..692
                     /locus_tag="PKH07_10940"
     CDS             <1..692
                     /locus_tag="PKH07_10940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35501.1"
                     /translation="VPNLLMPYGCPDHFCPSFHLYTFSPGTLQDVLLRSGLSIVKIDV
                     DSCWIKVLAERTPDADSILLRFAEQQIKACLGRLSFKEHSVDEMVECLGHCSADAQRL
                     QKAIDCFTTELGEAWAEFQQRHQNLLDSVKQETVSPAVLDQMFRGLFFCITRFEKTLG
                     MRDWAPIALAEIAKTTAHFTHLQAHETDINRRCQYEVQKYIQLRGILSKAVAETNQRL
                     KQIQFKIKLAS"
     gene            complement(817..>2271)
                     /locus_tag="PKH07_10945"
     CDS             complement(817..>2271)
                     /locus_tag="PKH07_10945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015722315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNT35502.1"
                     /translation="HLYSLEDDSRLVFAGANPAADQILGVDNAQFIGKTIEEAFPPLV
                     QTEIPDRYRRAARDGDIWQTEETQYEHGAIKGSFEVHAFQISPGKVAVLFNDTTERKT
                     LEAQLLQAQKMESIGRLAGGVAHDFNNMLNVILGHAELAVDQIDPMSPLFGDIQEIRK
                     AAKRSADLTRQLLAFARKQTISPKELDLNDAVEAMLTMLRRLIGENIDLVWLPGARAS
                     RVRIDPSQLDQILANLCVNARDAIGEVGKVTIETANVAFDEEYCASHVDFTPGNYVMI
                     AFSDNGCGMEKQTLAHIFEPFFTTKDIGKGTGLGLATVYGIVKQNEGFVNVYSEPGHG
                     TTFKIYLPRLKAPAEQDKTEKLGKTAQGGTETLLVVEDEPSILNMSVIMLKKMGYRVL
                     SAGSPLEAIRVAENCKDEIHLLMTDVVMPEMNGRELAQQLQAKFPGLKRLFVSGYTEN
                     VIAHHGVLEPGVFFLQKPFSKDELACALRRVLDA"
BASE COUNT          537 a          606 c          579 g          549 t
ORIGIN      
        1 aagtgcccaa ccttcttatg ccgtatggtt gccctgatca tttctgtccc agtttccatc
       61 tgtacacgtt ctccccagga acacttcagg atgtgctgct tcgaagcgga ctgagcattg
      121 tcaagattga cgtggactcc tgttggatca aagttctggc agaaagaaca ccggatgcgg
      181 actcaattct gttgcggttc gccgagcaac aaatcaaggc atgtctcgga cgcctctcgt
      241 tcaaagaaca ttccgtggac gaaatggtcg aatgtctcgg ccactgctct gccgatgcgc
      301 agaggcttca gaaagctatc gattgcttca ccactgagtt gggagaagcg tgggctgagt
      361 tccagcaacg ccatcagaat cttctggaca gtgtgaagca ggaaacggtc tccccagccg
      421 ttttggatca aatgttccga ggactgttct tctgtatcac acgttttgag aaaacgctgg
      481 ggatgcgaga ctgggctccc atcgccttgg cggaaatcgc aaaaacgacg gcgcatttca
      541 ctcatcttca ggcacatgaa accgacatta acagacgctg ccaatatgag gtacagaaat
      601 acatccagtt gagaggcatt ctgagcaaag ccgttgcgga aacaaaccag cgactgaaac
      661 agattcagtt caagatcaaa ctcgcttcat gatccatgag ggttcgccga tgttctcttt
      721 ggcgcaacac gcactcgcga aagtagccgt ctcttccgaa gtctggtttt cctgaaactg
      781 cggtctacca agaagagagc agtgcagaat ttctgactag gcgtccagca cccgacgcag
      841 tgcgcaggct agttcgtctt tggagaaggg cttctgaagg aagaagaccc ctggttccag
      901 aactccatgg tgggcaatga cattttcagt gtagccggaa acaaacagcc gtttgagccc
      961 agggaacttc gcttggagtt gttgtgcaag ttcccgtccg ttcatctcag gcatgacgac
     1021 atcggtcatc agcagatgaa tctcatcctt gcagttttcg gcaacacgga ttgcctcaag
     1081 gggactcccc gcagacagaa ctcggtaccc catcttcttc agcatgatga cactcatgtt
     1141 gagaattgat ggttcatctt caactacaag cagcgtctcc gtaccacctt gggcggtctt
     1201 gccaagtttc tcggtcttat cctgttcagc tggagccttg agccgtggca ggtatatttt
     1261 gaacgtggtt ccatgtcccg gttcactgta gacattgacg aacccctcgt tctgcttgac
     1321 gattccgtag acggtcgcca gtccgagacc tgtgcccttg ccaatgtcct ttgtcgtaaa
     1381 aaacggttca aagatgtgcg ccagagtctg tttctccatc ccgcaaccgt tgtcactgaa
     1441 agcgatcatc acatagttgc ccggtgtgaa gtcaacatgc gacgcacaat actcttcatc
     1501 gaacgcgaca tttgccgttt cgatggtcac cttacccacc tcgccaatgg catcccgagc
     1561 attgacacag agattggcga gaatctgatc caactgagac gggtcaattc tgacccgaga
     1621 ggcacgggcg ccaggcagcc agaccagatc aatgttctcg ccgatgaggc gccgcagcat
     1681 tgtgagcatg gcctccaccg catcattcag atcgagttct ttgggtgaga tggtctgctt
     1741 tcgggcaaag gcaagaagct ggcgcgtcag gtcagcggag cgtttggcgg ccttccggat
     1801 ttcctgaata tccccaaaga gtggactcat cggatcgatc tgatccacgg ccaattccgc
     1861 gtgaccgagg atcacgttga gcatgttatt gaagtcatga gcaacaccgc ccgccaggcg
     1921 tccgatagac tccatcttct gagcctgcaa gagttgtgcc tcaagtgtct ttcgttcggt
     1981 agtgtcattg aacaagacgg ccactttgcc aggtgaaatc tggaacgcat ggacctcaaa
     2041 agatcccttg atagcaccgt gctcatattg tgtttcttca gtctgccaga tgtcaccatc
     2101 acgtgccgca cggcggtagc gatccggaat ctccgtctgt accagtggcg gaaatgcttc
     2161 ttcaattgtc ttgccgatga actgagcatt gtcaacacca agtatttgat ctgcggcggg
     2221 gttggctccg gcaaaaacaa gacgactgtc atcttcgaga ctgtaaagat g
//
LOCUS       DAOQUC010000638         2270 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_59183_length_2270_cov_2.725959, whole genome shotgun sequence.
ACCESSION   DAOQUC010000638 DAOQUC010000000
VERSION     DAOQUC010000638.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2270
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59183_length_2270_cov_2.725959"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2270)
                     /locus_tag="PKH07_10950"
     CDS             complement(<1..>2270)
                     /locus_tag="PKH07_10950"
                     /inference="COORDINATES: protein motif:HMM:NF019193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-N-acetylglucosaminidase domain-containing
                     protein"
                     /protein_id="HNT35503.1"
                     /translation="DTEFYLIRPNEAGENLQEFFTPFLGAETWGQIPEEGFLIVSGLQ
                     DYENFVFLVSKSERGFRYASQAVVDLMLRLGDRFHLPRGFVLDYPDFGKRGVIEAFSG
                     PQWSESSRLDVIRFMARMRMNTYIYAPAGDEHINAKWFEPYPEEHRKRLGTLVNVAEE
                     NRVEFVFGVRPGPMIRYARVEDCNLLCDKYRTAVEMGVTHFALIFDQTVQELGHAEDK
                     NTYRSLAEAHADLANKVLAFLRQQFQGAELIVCPASANQSAPSPYHIVLGSNLDPAIS
                     LFWMGGVDPTGTWDNYQIRASDAKRMKSAVNRPLFVWDNFPSNELARNRLHMGPVSGR
                     SPELSTEVLGFVANPMNEAMASTVPLATIADFLWNSFDYSSEVSFRRACNWVGGSLAG
                     EDLHFFALQCQNSFLWNPASSEVSHQKEVLKMLQAVGQDALFDPTPLSDLWRQYQMVG
                     DNLSWKLQDKGLYKEIEPYLQLLRLYGETGPVCIKLLQLASSRESASANRELWENFLL
                     LSEKRNNSARFGRTIGGSSMSALIQEAFLSGIRALGAELPKVQANLEAYIDFTIDRAL
                     DNDRQTFFWSNRTLRDGDYVTLELPRASVVEKVEILQGEPSRPDDFVQQGVLEGSLDG
                     LKWLQLASLAQPEETVRLEAKALSFLRIRCTADQDKRLLLREFRVYSGSPIEIAMKPT
                     KLLAGSLASLVNGNLLDSCSLLSGLEKGTSLEIDLVQTKEISSILVYQDREKYYVGFR
                     IESSADGQNWSSHSTD"
BASE COUNT          547 a          615 c          589 g          519 t
ORIGIN      
        1 gatcagttga atgcgaggac cagttctgac catctgcaga actctcgatt cgaaacccga
       61 cgtagtattt ctcacgatcc tgatagacaa ggatcgaact gatctccttg gtttgtacca
      121 gatcaatctc caggctggtt cctttctcca gtcccgaaag cagcgaacag ctatccaaaa
      181 gattaccgtt gaccagactg gcgagacttc ccgcaagcag ttttgtcggc ttcatggcaa
      241 tctcgatcgg agaacctgag tagactcgga attccctcag aagcagtcgc ttgtcctggt
      301 cggcagtgca tcgaattctg aggaatgaca gggctttggc ttcaagacgg acggtttcct
      361 ccggctgagc cagagaagcc agttgaagcc acttgagacc gtccaaggaa ccttccaaga
      421 ctccctgctg gacaaaatca tccggtctgc ttggctcgcc ttggagtatc tcgactttct
      481 ctaccaccga agccctgggc agttcgagtg tcacgtagtc cccgtcccgc agagttcgat
      541 tgctccagaa gaaagtctgc cgatcattgt ccagcgctcg atcaatcgtg aagtcaatgt
      601 aggcttcgag gtttgcctgc actttgggca actccgcccc caaggctctg ataccggaga
      661 gaaacgcttc ctgaatgagc gccgacatgg aagaaccacc gatggttctg ccgaagcgag
      721 cgctgttgtt acgtttttca gacaagagca gaaaattctc ccacagttct cgattcgctg
      781 aggcggactc ccgacttgaa gcgagctgca gcagtttgat acagacgggt cctgtttctc
      841 catacagtct gagcagctgc aaataaggct cgatctcttt gtaaaggcct ttgtcctgga
      901 gtttccaaga caggttgtcg ccgaccatct gatactgccg ccaaaggtcg gacaggggag
      961 tcggatcgaa aagcgcatcc tgaccgactg cctgaagcat cttgagaact tccttttggt
     1021 gactgacttc tgaagaggcg ggattccaga gaaaggagtt ttgacactgg agcgcaaaga
     1081 aatgcagatc ctctcccgca agagaaccgc caacccagtt gcaggctcta cggaaggaga
     1141 cttctgaact gtagtcgaaa ctgttccaaa ggaaatcggc gatggtcgcc aagggtaccg
     1201 tcgaagccat ggcttcgttc atgggattgg cgacaaaccc caagacttcg gtgctgagtt
     1261 ccggcgatct tccactgacc ggacccatgt gaagtctgtt acgagccagc tcattggacg
     1321 ggaaattgtc ccagacgaac agaggacgat tgacagccga tttcatacgc ttggcatcgg
     1381 atgcacggat ttgatagtta tcccatgtgc cagtcggatc cacacctccc atccagaaaa
     1441 gggagattgc aggatccaag ttactgccca gtacaatatg gtagggcgag ggagcactct
     1501 ggtttgcact cgcaggacag acgatgagtt ccgctccctg aaactgctgc ctgaggaaag
     1561 cgagcacttt gtttgccaga tcggcgtgtg cttcggcaag gcttcggtat gtgttcttgt
     1621 cctctgcatg cccgagttcc tgaaccgttt gatcaaagat gagggcaaaa tgcgtgactc
     1681 ccatttcgac ggcagttcgg tacttgtcac aaagcagatt gcagtcctcc actcgtgcat
     1741 agcgaatcat cggtcctggt cgcactccga agacaaattc cacccgattc tcttcggcaa
     1801 cgttgacaag tgttcccagc ctctttcggt gttcttccgg atagggctca aaccatttag
     1861 cgttgatatg ctcgtcaccc gccggagcat agatatatgt gttcatccgc atccgtgcca
     1921 tgaaacggat gacgtccagg cggctgctct cagaccattg cggaccggag aaagcctcga
     1981 tgactccgcg cttgccgaaa tccggatagt ccaggacaaa gccacggggg agatgaaaac
     2041 gatcacccag ccggagcatc aaatcaacga ctgcctggga tgcgtatcga aaacctctct
     2101 ctgatttgga gaccagaaac acgaagttct catagtcctg cagtccggaa acgatcagga
     2161 agccttcttc aggaatctgt ccccaggttt ctgctcccag gaaaggcgtg aagaactcct
     2221 gaagattctc tcccgcctcg ttaggccgaa tcagatagaa ctcggtgtcg
//
LOCUS       DAOQUC010000639         2268 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_59251_length_2268_cov_3.383190, whole genome shotgun sequence.
ACCESSION   DAOQUC010000639 DAOQUC010000000
VERSION     DAOQUC010000639.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2268)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2268)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2268
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59251_length_2268_cov_3.383190"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..861)
                     /locus_tag="PKH07_10955"
     CDS             complement(<1..861)
                     /locus_tag="PKH07_10955"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HNT35504.1"
                     /translation="MRRRTGNPGYQYIILTFVLLLALFGVVLVFSSSASVAMMKNRDV
                     ISYARKQLLSLSIGLAILLACWAVDYMKILRLSRILMVGTFVLLIAVLFIPSAANVNR
                     SIRILGFSIIPSEFAKLTIILYLAATFARKQDQMGSFWRGPFFQLLVLGAACLLVFVQ
                     PSHTNAIFLGLMGLVLWFYAGAPKKHLVPLVVVFGLVSAFFIAQHEYAMGRIDKFVQA
                     VLNPLSPTSHHHSLQSTMSVINGGLFGVGLGNSEHALLYLPEPHTDFIWAILCEELGF
                     VSSSVVLLLYF"
     gene            complement(858..2237)
                     /gene="murD"
                     /locus_tag="PKH07_10960"
     CDS             complement(858..2237)
                     /gene="murD"
                     /locus_tag="PKH07_10960"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HNT35505.1"
                     /translation="MEQRMLPCREPLPPVAGSRVLVLGAGKCGLAASRLLHKAGAEVI
                     LSDSKSLDHLPEEVIQLQKLGIQIETGGHTLPANTGLIMRSPGVPKHIEVLRQAREEG
                     VAIVSEIEVGSWFCAPRIIAITGTDGKSSVVTMVNQALNSSGWKSVEAGNIGTPLCEV
                     VFDDCGRKHDFVVVEVSSYALENTYHFHPFVSVILNVAEDHLDHYESFEEYAETKGAI
                     RRNQTEEDFLVLNAQDQWCLRFAEKSAATVRWFSLDDHGRDGAFLSESTVVYREGRSE
                     RTRHISLQDWFRHQKENLLASVVTTVLCGSEMESVVDALGQFRGLPHRIEFVRQLRGV
                     TFYDDSKATTPHATQAALKSMERPVILIAGGDAKGTDITSLRPFIQEKVKSLVLLGKS
                     RDLFVERFSDCVECQKVDSMQQAVTEAFLKAKKGDVVLLSPACSSLDMFRNFEQRGQI
                     FKEAVATLL"
BASE COUNT          589 a          605 c          573 g          501 t
ORIGIN      
        1 gaaatacagc agaagaacaa cggatgaaga aacaaaacca agctcttcgc agagaattgc
       61 ccagatgaag tccgtgtgag gttcgggtaa atagagaagc gcgtgttctg aattgcccag
      121 accgacgcca aacagcccac cgttgatgac agacatggtt gactgcaaac tgtggtggtg
      181 actggttgga gaaagaggat tgagaaccgc ctgaacaaac ttgtcaatgc gacccattgc
      241 gtactcatgc tgtgcaatga aaaacgcact gacaagacca aagaccacga ctaagggaac
      301 caaatgcttc ttgggtgctc cggcatagaa ccaaagcaca agacccatca ggccaaggaa
      361 gatggcattg gtgtgactgg gctgcacaaa taccaacagg caggccgcgc caagaaccag
      421 gagttggaag aacggacctc gccagaagga tcccatctga tcctgcttgc gggcaaaggt
      481 tgctgccaag tagaggatga ttgtgagctt ggcaaattct gaaggaatga tactgaatcc
      541 aagaatccta attgaacgat tgacgttggc agccgacgga atgaagagaa ccgcgatcaa
      601 cagcacaaaa gtgccaacca tcagaatccg gctgagtctc aggatcttca tataatcaac
      661 tgcccagcat gccagaagaa tagcaaggcc gatgctcaag gacagcagct gtttgcgagc
      721 gtaggaaatc acatcccgat tcttcatcat cgcaacggaa gcagaactgc tgaagaccaa
      781 caccactccg aacagcgcca gaagaagcac aaaggtcaga atgatgtatt ggtatcctgg
      841 atttcctgtt cgccttctca taagagtgtc gccactgcct ccttgaagat ttgtccccgc
      901 tgctcgaaat tcctgaacat atccagactg ctgcatgcgg gagaaagcag cacgacatcg
      961 ccctttttgg ctttcaagaa ggcttctgtg actgcctgtt gcatggagtc caccttctga
     1021 cactcgacac aatccgagaa tctttccacg aaaagatctc tgctttttcc cagcaacacg
     1081 agcgacttga ccttctcctg aataaaaggt cttaaagagg tgatatccgt gcccttggcg
     1141 tcgccaccgg caatcagaat tacaggccgt tccatggatt tcagagcggc ttgagtcgca
     1201 tgaggcgttg ttgctttgga gtcgtcgtag aaggtcacgc cgcgcaactg tcgcacgaac
     1261 tcaattcggt ggggcaagcc tcgaaactgt ccgagtgcgt ccacgacgga ctccatctct
     1321 gatccgcaga gaaccgtcgt aaccaccgat gcaagaaggt tctctttctg gtgtctgaac
     1381 cagtcttgca gtgagatatg tcgggttcgt tccgatcgcc cttcgcggta gacaaccgtg
     1441 ctttcggaga gaaaagctcc atcgcgacca tgatcatcga gggagaacca tcgaactgtg
     1501 gccgctgact tctcagcgaa tctcagacac cattgatctt gggcgtttag gaccaggaaa
     1561 tcctcctcgg tctggttgcg acgtattgcc ccttttgtct cagcatattc ctcaaatgac
     1621 tcatagtgat cgagatgatc ctcagccacg ttgagaatga cgctcacgaa cggatggaaa
     1681 tggtaggtat tctccagcgc gtaactactg acttcgacca cgacgaaatc gtgctttctt
     1741 ccgcagtcgt caaacacgac ttcgcagaga ggagttccga tgttgccggc ttccacgctt
     1801 ttccatccgg aagagttcaa ggcctgattt accatggtca ctacggatga tttcccatcg
     1861 gtgccggtta tcgcgatgat tcttggagca cagaaccagc tgcccacttc gatctccgaa
     1921 acgatggcca cgccttcttc tctggcttgg cgcagtacct caatgtgttt cggaacgcca
     1981 ggacttctca tgataagtcc tgtattggct ggaagcgtat gtccacccgt ctcaatttgg
     2041 attcccagtt tctgaagttg aatgacctct tcaggaaggt gatccagaga cttggagtcg
     2101 gaaaggatga cttcagcccc agccttgtgc aggagccttg acgccgccag gccacacttg
     2161 cccgcgccga gcaccagaac tcgtgagccg gcaacggggg gaagaggttc ccgacacggc
     2221 aacatccttt gctccatcag cccattctcc tcagcgcatc ttcagtgt
//
LOCUS       DAOQUC010000640         2266 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_59335_length_2266_cov_3.132972, whole genome shotgun sequence.
ACCESSION   DAOQUC010000640 DAOQUC010000000
VERSION     DAOQUC010000640.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2266
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59335_length_2266_cov_3.132972"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            119..2071
                     /locus_tag="PKH07_10965"
     CDS             119..2071
                     /locus_tag="PKH07_10965"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNT35506.1"
                     /translation="MKHNRFTIWVCTVVLFTVSFLSVFLTQDDYGPHWDAHTYYPPAS
                     RFGMWMRSHWTGEDVPSISDIDAYFPWNRDHPPLGKILMGMTYALFAKTIGEADATGL
                     AVLLCFATLVSTVFLVCHDSMGFMPALFGAMMMWFMPRVFCHAHLIALDLIVTPLILI
                     SVFAFAKNASRKLGFIVPGLCLGLALLTKLQAAFVPLILCFWLVWGCLLEKDQKNQRM
                     WTPWINLLLSGLLGMVILIAGWPFLWGDMRGNLKIYWEFLSVRKDLDVFYFGQRTAGW
                     TYPFVMTLITTPPAALITALFATRSFLQRKAHPSTRLWLCGIVVPLAVIAMPGVKRYD
                     GIRLFMPFLPFLVCLSAAGFRTILRHIEIRIPTVARQNILALAFLVLLPGSALIELII
                     YHPHQIVYYSPLIGGFPGAYLAGMDADYLGLCIQESLPVVHARVPKGSWLFLAGVNTA
                     NQFSPVSLPDPGAPVDLSLNSTYRVASVPMTQDILDSPWGMYTLINNRRGGLGEVGDI
                     IVSQQMPIWEYEFQGVPLLMLYAYPKRGAYDAGARYVFEHIEALDVLWHDDEEELQII
                     WRLRESAPSLGLFIDQVDSGRRVLERTHQERLYSLYPTEFWLPGDTIRTRYPILLQPR
                     TETFEIRIIERESEREEGLIRMKVSH"
     gene            complement(2046..>2266)
                     /locus_tag="PKH07_10970"
     CDS             complement(2046..>2266)
                     /locus_tag="PKH07_10970"
                     /inference="COORDINATES: protein motif:HMM:NF013835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35507.1"
                     /translation="ATTHNLFFFIDLMARIREAIRSGVFQQFKREFLTRYLNPNSGAP
                     HTTPATCCRRSRNERPVFDLTNETPSCE"
BASE COUNT          478 a          578 c          577 g          633 t
ORIGIN      
        1 aaagcccttc atagctgatg agcctccttt gaatatctgc atgctacctt aatctcgcat
       61 gtcgaagcta atctagagaa cttcacatgt tcatgtcaaa tgctatactg taccgaagat
      121 gaaacacaac agattcacga tctgggtatg cacggtagtc ttgtttaccg ttagtttcct
      181 aagcgtcttt ctcacgcagg atgactacgg tccccactgg gatgcacaca cctattatcc
      241 acccgcatct cgcttcggaa tgtggatgcg cagccactgg acaggcgaag atgtcccttc
      301 catctcggac attgacgcct atttcccttg gaatcgcgat catccgcctc tcggcaagat
      361 tctcatgggc atgacgtacg ctctttttgc caagacgatc ggagaggctg acgcaaccgg
      421 acttgcagtt ctgctctgct ttgccacttt ggtgtccact gtctttcttg tttgtcatga
      481 ttcgatgggc ttcatgcccg cgctgtttgg tgccatgatg atgtggttta tgcctcgggt
      541 cttttgtcat gctcatctga ttgcgctcga tctgatcgtg acacctctga tcttgatctc
      601 cgttttcgcg ttcgccaaga acgcctcacg caaactgggt ttcattgttc caggtttgtg
      661 tcttggactt gcattgctca cgaaacttca ggcagcgttc gttccgttga tcctttgttt
      721 ctggctggtc tggggatgcc tactggaaaa ggatcagaag aatcaaagga tgtggacgcc
      781 ttggattaat cttctgctat ccggattgct cggcatggtg atactcattg caggatggcc
      841 atttctttgg ggagatatgc gagggaatct gaaaatctac tgggaatttc tctccgtgag
      901 aaaggatctg gatgtcttct actttgggca gcgtaccgcg ggatggacct atccctttgt
      961 gatgacactg attacgactc cacctgcggc gctgatcact gcattgttcg cgacaaggag
     1021 tttccttcag cgaaaggctc atccctcaac tcggctgtgg ctatgcggta ttgtggttcc
     1081 gttggccgta atcgcaatgc caggtgtcaa acgctatgac ggtatcagac tcttcatgcc
     1141 ctttctccca tttttggtat gtttgagcgc ggccggcttc cggacaatcc ttcggcatat
     1201 tgagatcagg attcccactg ttgccagaca gaatatcctt gcgcttgcgt ttctggtgct
     1261 cctaccaggt tcggcgttga tcgaactcat catctatcat ccccatcaga ttgtttacta
     1321 cagccccctg atcggcggat ttcctggggc gtatcttgcg ggtatggatg cggactactt
     1381 gggcttgtgt attcaggagt ctctacctgt cgtgcatgcg agagtcccaa aaggttcatg
     1441 gctttttctc gcaggggtaa acacagcaaa ccagttctct cccgtctctc tgccggatcc
     1501 gggtgcgccg gtggatctgt ctttgaacag cacgtaccga gttgcttctg tccctatgac
     1561 tcaggacatt ttggactcac cctggggaat gtatactctg atcaacaaca ggcgtggtgg
     1621 cttgggggaa gtcggcgata tcatcgtcag tcagcagatg cccatctggg aatacgaatt
     1681 ccagggcgta ccgctcctta tgctctatgc ctatccgaag cgaggagcct acgatgcagg
     1741 ggcccgatac gtcttcgagc acattgaggc tttggacgtt ttgtggcacg atgacgaaga
     1801 ggaactgcag atcatctgga gactccgaga atcagcaccg tctcttgggt tgtttattga
     1861 ccaagtggac tctggaagac gtgtcttgga gagaactcat caagagaggc tctactcgct
     1921 ctatcctacg gagttctggc tccccggtga cacaatccga actcggtatc ccatcttgct
     1981 ccaacctcga acagagactt tcgagattcg gataatcgag cgcgaatcgg aaagggaaga
     2041 ggggctcatt cgcatgaagg tgtctcatta gtcaaatcga atacaggcct ttcattccga
     2101 ctccgtctgc agcatgtggc aggcgtcgtg tgaggcgcac cagagtttgg attcagatac
     2161 cttgtcagaa actcccgctt gaattgttga aatactcctg aacgaatggc ctctctgatc
     2221 cgtgccatca gatcgatgaa gaaaaagaga ttgtgcgttg tcgcaa
//
LOCUS       DAOQUC010000641         2266 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_59339_length_2266_cov_2.970602, whole genome shotgun sequence.
ACCESSION   DAOQUC010000641 DAOQUC010000000
VERSION     DAOQUC010000641.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2266
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59339_length_2266_cov_2.970602"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..410)
                     /locus_tag="PKH07_10975"
     CDS             complement(<1..410)
                     /locus_tag="PKH07_10975"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNT35508.1"
                     /translation="MGKEIVHSPEQDQESIWIEKARLGNQEAFGKLVKVHMKAAYYSA
                     LNLVRNHDDALELSQEAFLRAFKNMSTFRQGSRFYPWLYKILRNLCLTHLRRKVRHPD
                     SISLSDEEGDWDFPADTPDASEQMEQTEMEERIWA"
     gene            649..1929
                     /locus_tag="PKH07_10980"
     CDS             649..1929
                     /locus_tag="PKH07_10980"
                     /inference="COORDINATES: protein motif:HMM:NF018743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HNT35509.1"
                     /translation="MTFLAKLIVGVALFAIGTANVYAVTVVEPKVKLGVENFCEDGPP
                     GGLVGAKFGLLTNQSGLDSQFRSTIDLLHQRKDMKLVILFGPEHGIRGDRFDARHEDE
                     MVDDKTGIKVYSVFGSSRSKFEERLEGLDALIYDIQDVGSRGYTYVSSMIDAMEKCAR
                     KKVKFLVFDRPNPLGGNRVEGNVLEKELISFVGCAEMPYVYGLTPGELATWIKAERKI
                     DVDLTVIPLKGWRRDMTWEETGLPWIPTSPHVPHPSVCYHMVVTGVLGELHAVNEGVG
                     TAAPFEYMGAPWIDSVKLADYLNAAEIPGFYFRPASFIPRYHSYKEEQCQGVQIHLVN
                     MKTANTWQANLALMTALHKLYPEKKIFEGELAPQRIDMFEKVCGTRRIRADILAGKSA
                     KEIDAQFDADRQAWIGKVAPFIIYKEGNETFKVE"
BASE COUNT          540 a          538 c          638 g          550 t
ORIGIN      
        1 gcccaaatgc gctcttccat ctccgtctgc tccatctgtt cggaagcatc cggagtgtca
       61 gcagggaaat cccaatcccc ttcttcatcg ctcagtgaga tgctgtcggg gtgtctgacc
      121 tttcgacgca gatgcgtcag gcagagattc ctgagaatct tgtacagcca gggatagaaa
      181 cgggaacctt gtcggaatgt gctcatattc ttgaaagccc gaaggaaggc ttcctgggaa
      241 agttccaacg catcatcgtg attgcgtaca aggttcaacg cagaatagta tgcggccttc
      301 atgtgaacct tgaccagttt tccaaaagcc tcttgattgc ccaagcgggc tttctctatc
      361 cagatgcttt cctgatcttg ttcaggtgaa tggacgatct ctttgcccaa acccgattct
      421 ccaaaacagt caatgaatca cgcgacgaga gtaaaataca tcgtttgaag aatctcgtcc
      481 agacgatttc gggactagat tcagatctgt atttcgaggc actcctcggg ttattgttgc
      541 cttgactgtt cccgcctatc atccgggttg acaaatccgt actttggccc ctactcttgg
      601 caattgggat cgaaaagtga gaaagctgat tgagaggaga ataagaagat gacgttcttg
      661 gccaagttga ttgtgggagt cgcccttttt gccatcggta ctgcgaatgt ctacgcggtg
      721 acggttgtgg aaccaaaggt caaactggga gtggagaatt tttgtgaaga tggccccccg
      781 ggtggtctgg tgggagccaa gttcggtctc ttgaccaatc agtcggggct ggactcgcag
      841 tttcgaagca ccattgacct gctgcatcaa aggaaagaca tgaagctggt cattctgttc
      901 ggccctgaac atggcattcg gggtgaccgt tttgatgcac gtcatgagga tgagatggtg
      961 gatgacaaga cgggcatcaa ggtctacagc gtgtttggct catccagaag taagtttgag
     1021 gagaggctcg aagggctcga tgcgttgatc tatgacattc aggacgtcgg cagccgcggc
     1081 tacacatacg tctcgtccat gattgacgcc atggagaagt gcgccagaaa aaaggtgaag
     1141 ttcctggtct ttgacagacc caatccgctc gggggaaatc gtgttgaggg caacgtcctg
     1201 gagaaagaac tgatttcctt tgtgggttgt gcagagatgc cctacgttta tggtttgacg
     1261 cctggggaac tcgccacctg gatcaaggcg gaacggaaaa ttgacgtcga tctgactgtc
     1321 atccctttga aaggctggcg tcgagacatg acttgggaag agactgggct tccttggatt
     1381 ccgacgtcac cccatgtgcc tcatccaagc gtctgttacc acatggttgt gacgggagtt
     1441 ttgggcgaac tgcatgcggt caacgagggc gttggaacgg cagctccttt tgaatacatg
     1501 ggtgcgccgt ggattgactc ggtcaaactt gccgactatc tgaacgccgc tgagattcct
     1561 ggcttctact tccgaccggc ttcgtttatc ccgcggtacc atagctacaa agaagagcag
     1621 tgtcagggcg tccagatcca tctggtgaat atgaagaccg ccaatacgtg gcaggctaat
     1681 ctggcgctca tgactgcgct tcacaaactc tatccggaga aaaagatctt cgagggagaa
     1741 ttggctcctc agaggataga catgtttgag aaggtttgtg gcaccaggag aatccgtgcg
     1801 gacattctgg caggcaagag cgcgaaggaa attgatgctc agttcgacgc cgatcgccag
     1861 gcatggattg ggaaagtggc acccttcatc atctacaaag agggcaacga aaccttcaag
     1921 gttgagtaga tgaataatgg aagggtaatc catgcttcgg tagcaaggct ctgcgcgctc
     1981 ccgaagcaac aaatcccccc ttcccccctt gttaaggggg atcataagaa ccccccctta
     2041 ttaagggggg atcaaggggg gattctgtga cgagccggag gcagaaaggt tcgtagtatt
     2101 cgtagtatgc tcgtaagggc attttcagga tcacgcctta cggcgtaact acgaacgaaa
     2161 acgcggcatg aatgctgtgc gaaggctctt gtcatcctga accgttcgct ctgctgccga
     2221 ctcgtaggaa gttggacgga tccctggttt gaaggtgttc gccatg
//
LOCUS       DAOQUC010000642         2264 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_59410_length_2264_cov_3.810774, whole genome shotgun sequence.
ACCESSION   DAOQUC010000642 DAOQUC010000000
VERSION     DAOQUC010000642.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2264
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59410_length_2264_cov_3.810774"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1047)
                     /locus_tag="PKH07_10985"
     CDS             complement(<1..1047)
                     /locus_tag="PKH07_10985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35510.1"
                     /translation="MNGVSPDQNLPTAAGPAEVSGSHLKAEDSLCQKQTEREQRRATH
                     GLDAIIVFLVPLMLAVAFFLKACFPLESCNDVWWHLKTGKFLLEYWDQHGFGFPENDV
                     FSVSGETLPWANHEWLADVLIFLGYDQFGAMGVVALKALTITLTYLLLYWLLLRVIRP
                     SDPAKTETTSPATHSALAGVGCILAFMTSQYTMYLRPPVLTYLFLVIQLHLMWNLRTG
                     QQPISKRLSFWAMPVMMVPWVNLHGGAILGIVLPGLYAMGIALDGIWNRVRESRPLPF
                     SEFGSWALLTGLMFLASLCNPFGYDILMLTAKIMRSPRLIQLISELQSPNFHFTRDYE
                     VLIVLLIGLGVLGRR"
     gene            complement(1044..1292)
                     /locus_tag="PKH07_10990"
     CDS             complement(1044..1292)
                     /locus_tag="PKH07_10990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35511.1"
                     /translation="MTVDLRHADKKERPWGAYFGAAVAFLILTWAVCAILLPIVDPVE
                     ARDKLGIDRVRIMLQEEHSGARPVKPAPDPTMQSEGTP"
     gene            1470..>2264
                     /locus_tag="PKH07_10995"
     CDS             1470..>2264
                     /locus_tag="PKH07_10995"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004092819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HNT35512.1"
                     /translation="MKRTPVKKVVLAYSGGLDTSVIIPWLRENYGCEVIAYAADVGQG
                     EELAPLEEKGIKTGASKVIIKDLRDEFIREFCFPMMQAGALYEGQYLLGTSIARPIIA
                     KHQIAVALEEGADAIAHGATGKGNDQVRFELTAKALAPHLKVIAPWREWDLKSREDCM
                     EYASKRGIPVPVTVRKPYSMDRNLWHLSFEGGVLEDPWNEPPEDMFVLTTDPRKAPDQ
                     PTKLDIDFENGIPVRVNGKTYGAVELVTLLNEIAGSNGVGRVDIVEN"
BASE COUNT          562 a          591 c          621 g          490 t
ORIGIN      
        1 ccgtcgtccc aatactccaa gcccaatcag aagcactatg agcacttcat agtctctggt
       61 gaaatggaag ttgggagact gaagctcact gatcaactga atcagacgag ggcttctcat
      121 aatcttggcg gtcaacatca gaatgtcata gccaaacgga ttgcacagag acgccaggaa
      181 catcaggccg gtaagcagag cccaactacc gaactccgaa aatgggaggg gtctgctctc
      241 gcgaactcgg ttccaaatgc catccagggc gatccccatc gcatagagcc cgggcaggac
      301 aatcccgaga atcgctccac cgtgcagatt cacccacggc accatcatga ctggcatcgc
      361 ccagaaactc aggcgcttcg atatcggttg ctgtccggtt cgaagattcc acatcagatg
      421 cagttggatg accagaaaca gatacgtcag aaccggtgga cgaagataca tcgtgtactg
      481 ggaagtcatg aacgccagga tgcagccgac accagccaac gcggagtgtg tcgccggcga
      541 tgtggtttct gtctttgcag ggtccgaagg tctgatcact ctcagaagca gccagtacag
      601 cagaagatag gtcagggtga tcgtcaaggc tttcagagca acgacaccca tggcaccaaa
      661 ctgatcatag ccgagaaaga tcaacacatc ggcgagccat tcatggtttg cccaaggcag
      721 agtctcgcct gacaccgaga acacgtcatt ttcgggaaac ccaaagccgt gctgatccca
      781 gtattcgagc aggaactttc cggtttttag atgccaccag acatcattgc agctctccaa
      841 tggaaaacac gccttgagga agaaagccac ggcaagcatg agcggaacca ggaacacaat
      901 gattgcatca aggccatgcg tcgctcgtct ctgttcacgc tctgtctgct tttggcacaa
      961 cgagtcttcc gctttcagat gagaaccgct gacctctgcg ggcccggcag cagtgggcag
     1021 attctggtca ggtgataccc cgttcatggg gttccttcac tttgcattgt tgggtctgga
     1081 gcaggtttga caggtcgggc tcccgaatgc tcttcctgaa gcatgattcg cacacggtca
     1141 atgccgagtt tgtcacgagc ctcaacagga tcaacaatcg gcagaaggat ggcacagacg
     1201 gcccaagtga gaatcagaaa cgcgacagca gcaccaaagt aagcgcccca cggtctttcc
     1261 tttttgtcag catgtcgtaa gtcaacagtc atcttgcctc ttgcccttct cctttgaaca
     1321 ctgctgcaat cgtaatcaga acaagagcag aaagtcaagg cgcggattat gggtgttccg
     1381 tttggcagta ctcagctcgg gttgctttcc tccgacagat ttgatataca tcattttttg
     1441 gtcattttga cgaaatgcgg agaaagagca tgaaaaggac tcctgtgaag aaggttgttt
     1501 tggcctattc gggtggactg gatacttcag tcatcatccc ttggctgcgc gagaactatg
     1561 gctgtgaagt cattgcgtac gctgcggacg ttggtcaggg agaagaactg gcgccgctcg
     1621 aggaaaaggg aatcaagacc ggtgccagca aggtcatcat caaggacttg cgggatgagt
     1681 tcatccgtga attctgcttt ccgatgatgc aggccggggc gctttacgaa ggtcagtatc
     1741 tgctcggtac ttccattgcc cgaccgatta ttgccaagca ccagatcgcc gtcgcgcttg
     1801 aggaaggtgc cgatgccatt gcgcatggtg ccaccggcaa gggcaacgat caggtgcgtt
     1861 ttgagttgac tgccaaggcg cttgcacccc acctgaaggt tatcgcccct tggcgggaat
     1921 gggatcttaa gtcccgcgaa gactgcatgg aatacgccag caaacgaggt attcccgtgc
     1981 cggtgacggt tcgcaagcct tacagcatgg atcgcaacct gtggcatctg tcatttgaag
     2041 ggggcgtgct ggaagaccct tggaatgaac caccggaaga catgtttgtt ctgacaacag
     2101 atccgcgcaa agctcccgat cagccgacga agctggatat tgatttcgag aatgggattc
     2161 ccgtccgtgt caatggcaag acctacggtg ccgtcgaatt ggtcacgctg ctgaacgaga
     2221 ttgctggcag caacggcgtt ggcagggtgg atattgttga gaac
//
LOCUS       DAOQUC010000643         2262 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_59524_length_2262_cov_2.508836, whole genome shotgun sequence.
ACCESSION   DAOQUC010000643 DAOQUC010000000
VERSION     DAOQUC010000643.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2262
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59524_length_2262_cov_2.508836"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..600)
                     /locus_tag="PKH07_11000"
     CDS             complement(<1..600)
                     /locus_tag="PKH07_11000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35513.1"
                     /translation="MLRIVLLDNDYLAISFPYNAALVERIKRLTTRRWNPDARQWEVH
                     LAHLPEIMAMLRLNQAMLPPNIRKAYAERWQGLDAVVRVSNDYSVLSGGKIPIDALDE
                     ETSYWKMGAEHTSSYKEGKWDGMRRLFDRRTASFPTGLLSRILKVLDRQQVRYRIEDL
                     RDRGTESLRFKKPSAAQLRQYQKEALAAALKGERGVLEMA"
     gene            complement(769..>2262)
                     /locus_tag="PKH07_11005"
     CDS             complement(769..>2262)
                     /locus_tag="PKH07_11005"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HNT35514.1"
                     /translation="LEELVGQTFLAKIIELRGRQDAVLSRREYLEEERDRRRKETLGL
                     LQEGALVRGVVKNLTDFGAFVDLGGIDGLLHINDMSWQHVTRPEDVVAVNQEVEVLIL
                     QIDGERISLGLKQKTEDPWGDVLDRYPIGAVVSGQITSLTKYGAFVELEPGVEGLIHI
                     SEMSWTHRIKHPSDVFNIGDMVAAQILKVDDANKRISLGYKQTQDNPWALAEVKYPVN
                     STVEGEVVGLTDFGAFVRLEDGVDGMIHVSDMSWTKKIKHPSEVLKEGDAVQAVVLEV
                     NGEQKRISLGLKQVEPDPWSRAKKRYRLGNHAEGTVVKVTDFGAFVELEEGIEGLIHI
                     SEISDRRVDHPEDMLKVGDRIHAKVIKSDSKNRKIGLSIREYQNDMERQEVQKYMDSG
                     EESLGTMGDLISSALKRYDMQAEKEAQKASQKADETSEEAAPQDIEPTVEVVPEPAAE
                     TAPEPETEPTEQPVSEQVVEPVEEDKSVEETTTPEETESENRATPTE"
BASE COUNT          486 a          644 c          553 g          579 t
ORIGIN      
        1 ggccatttca agaacgccgc gttctccctt gagcgccgcg gcaagcgcct ccttctgata
       61 ctgtctcaac tgtgctgcgg aaggcttctt gaatcgaagc gattcggttc cacgatcacg
      121 caagtcttca atgcggtagc gaacctgctg gcggtcgagc actttgagaa ttcgggagag
      181 tagacccgtt gggaaggatg ccgtgcgccg atcaaacagc cgcctcattc catcccattt
      241 gccttctttg taactggatg tgtgttcggc acccattttc cagtaggacg tttcctcgtc
      301 caaagcgtca atgggaattt tcccgccaga aagcaccgaa tagtcattgc tgacgcggac
      361 aacggcatcc aaaccctgcc aacgctctgc ataggccttg cggatgttgg ggggaagcat
      421 tgcctgattc agacgaagca tcgccatgat ctcaggcaga tgggcgagat gaacttccca
      481 ctgacgggca tctggattcc aacgacgggt cgtcagccgc ttgatgcgct ccacaagcgc
      541 ggcgttgtac ggaaaggata tcgcaagata gtcattgtcc aacagaacga ttcgaagcat
      601 aactgtccta ctgtagacca gattttcaaa gaaagcaaca tggcaagaac accatgcacc
      661 gccagcccca atatctctgt gtctcagggg acacattagg aaaaacccta aaggctcttt
      721 tggagacctt tagggttttt gtgttgctca gtctatcgag cgttttccct attcggtggg
      781 agtcgctcga ttttcggatt cagtttcctc gggtgttgtc gtctcttcga cagatttgtc
      841 ttcttcgaca ggctccacaa cctgttcgga aaccggttgc tcggtcggct ctgtctcggg
      901 ttccggagct gtttccgcag ctggctcggg aacaacttcc acagtcggtt cgatgtcttg
      961 aggggcggcc tcttcggaag tttcgtcggc tttctgagat gctttctgag cttccttctc
     1021 cgcttgcatg tcgtaacgtt tcaaggcgct ggagatcaaa tcgcccatcg taccgagact
     1081 ttcctcgcca gaatccatgt acttctgaac ttcctggcgc tccatgtcgt tctggtattc
     1141 gcgaatgctc aagccgatct ttctgttctt ggaatccgac ttgatgacct tcgcatgaat
     1201 acggtcaccg actttgagca tgtcttcggg atggtctaca cggcgatctg aaatttccga
     1261 gatgtgaatc agtccctcga tgccttcttc cagttcgaca aaagctccga aatcggtgac
     1321 cttgacaacc gtgccttccg catgattgcc gaggcgatat cgtttcttgg ctctcgacca
     1381 tggatcaggt tcgacctgct tcaacccgag cgaaatgcgc ttctgctcgc cgttgacttc
     1441 cagaacgacc gcttgaacag catccccttc cttgaggact tcggacgggt gtttgatctt
     1501 ctttgtccag gacatgtcag agacatggat catgccatcc acgccgtctt ccagccgaac
     1561 aaaggcgcca aaatcggtca ggccgacaac ttcaccctcc acggtcgagt tgacaggata
     1621 cttgacttcc gccagcgccc agggattgtc ctgagtctgc ttgtagccga gggaaattct
     1681 cttgttggca tcatccactt tgagaatctg cgcagcgacc atgtccccaa tgttaaacac
     1741 atctgatgga tgcttgatgc gatgagtcca ggacatttcc gaaatgtgaa tcagaccttc
     1801 tacaccgggt tccagttcta caaaagcgcc atacttggtc aggctcgtga tctgtcctga
     1861 cacaaccgct ccaatgggat atcggtcgag aacatctccc cacggatcct cggttttctg
     1921 cttgagacca agggaaatcc gctctccatc aatctgaagt ataaggactt ccacttcctg
     1981 attcacagca acgacatctt cgggtcttgt cacatgctgc caggacatat cgttgatatg
     2041 gagtagtccg tcaatgcctc ccagatccac aaatgcgcca aagtcagtga gattcttgac
     2101 cacacccctg acgagcgctc cttcctggag caagcccagg gtttcctttc gacgccggtc
     2161 tctttcctct tccaggtact ctcggcggga gagcactgcg tcctgtctgc cacgcaactc
     2221 gatgatcttg gcaagaaaag tctgccccac cagttcctca ag
//
LOCUS       DAOQUC010000644         2261 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_59541_length_2261_cov_3.940163, whole genome shotgun sequence.
ACCESSION   DAOQUC010000644 DAOQUC010000000
VERSION     DAOQUC010000644.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2261
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59541_length_2261_cov_3.940163"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1693)
                     /locus_tag="PKH07_11010"
     CDS             complement(<1..1693)
                     /locus_tag="PKH07_11010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35515.1"
                     /translation="MKFKYKTSEILVYRNSRQRLAVSRKEPEWRRNGLCLLGVILSFL
                     TALPTWAAPSFEMELLRFPSKLKAGGQSCLTARFTLDQPLETDSNVWVGFSQQDRLHF
                     VSEFHPKTSVRNWIPGKEYSLKIPFEIPADIHSGTYEMQIGLHTLASQSPNCFAVEIK
                     SATPTRTRPTLSHGVFSDRDGIPHRWYVNETNTLMWDGAPHLPVGGMFVPRSVGSEPV
                     ASDEKDASFQADEAIIDLIQSYGVRDLYLHYGTGPNCHPSRMQRLIDRLESKQMRYGI
                     EFGEDPGEGLRYLLRSENAAVENITKPGIHEIREQGWVGGLYLVLTADGQFVSAGEAI
                     KTPKGLAAEVVSCSPESPVSLFFVPRVRRGGHFWDGIYAQYETRVISYLQSLRFGSGF
                     RFIVDPFMNEMTIPYDAIPDSGEFRSGFARWLLERYATEEGLKEAWALHEVISFEQAS
                     SLVPLRVSSDSRGIGFLYSLSSQKVVTADMSTSQLWHDFVEYHATSVRTACNRIARAI
                     KSVADVPVVLKHHPQVDSYRINDTEGIGFDGIGMESYGTGELLKMCGLTSADIVET"
     gene            2009..>2261
                     /locus_tag="PKH07_11015"
     CDS             2009..>2261
                     /locus_tag="PKH07_11015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35516.1"
                     /translation="MNHLKCVCMLVLVVVCPTPAFCQPEPLAEGMISWEDFREFPIGS
                     LIPISNGMENECPTVAVDRKGTVWVAWIREVADGDQVWLK"
BASE COUNT          548 a          582 c          599 g          532 t
ORIGIN      
        1 tggtctcaac gatatcggcc gaggtcaggc cgcacatctt gagcagttcg ccggtaccat
       61 aggattccat tccgatgcca tcaaatccga tcccctctgt atcgttgatg cgatagctgt
      121 ccacctgtgg atggtgcttg agcaccaccg gaacgtcggc aacacttttg atggctcggg
      181 cgattcggtt gcaggcagtc cgaacgctgg tggcatggta ttccacaaaa tcatgccaga
      241 gttgggatgt gctcatgtcc gcagtgacca ctttctgact ggagagcgag tacaggaatc
      301 cgattcctct ggagtctgaa ctgactcgta ggggaacaag gctggatgct tgttcaaagg
      361 agatcacctc atggagagcc caggcttctt taagaccctc ttcggtggca tagcgctcaa
      421 gaagccagcg tgcaaaacca cttcggaact cacccgagtc cgggatggca tcgtagggga
      481 tggtcatctc gttcataaaa ggatcgacga tgaatcggaa gccgctccca aaccgcaacg
      541 actgcagata ggagatcact cgggtttcat actgggcata gatgccgtcc caaaagtgtc
      601 cgcctcttct cactctgggg acgaaaaaca aggacaccgg actctccggt gaacaggaca
      661 caacctcagc ggccagacct ttcggagtct tgattgcttc acctgctgag acaaattggc
      721 catcggccgt caataccaga tacagcccac cgacccaacc ctgttccctg atctcatgga
      781 tgcctggttt tgtgatgttt tcgactgccg cattttcact gcgcagcaga taacgaagac
      841 cttcacccgg atcttctcca aactcgatgc cgtaccgcat ctgtttgctc tcgagacgat
      901 caataagtct ttgcatacgg cttggatggc aattggggcc tgttccatag tggagataaa
      961 gatccctgac tccgtaagac tggatcagat caatgattgc ctcgtctgcc tgaaacgacg
     1021 cgtctttttc atcagaggca acgggttcgg aaccaaccga tcgaggaacg aacatgccac
     1081 caacgggtag atgcggcgct ccgtcccaca tcagagtgtt ggtctcattg acataccagc
     1141 gatgcggtat tccatctcga tctgaaaaga caccatggga gagtgtgggt cgagtgcgtg
     1201 tgggtgttgc agacttgatt tcgaccgcaa agcaattggg agactgagac gccagcgtgt
     1261 gcaaaccaat ctgcatttca taggttcccg aatggatgtc cgcgggaatc tcaaagggaa
     1321 tttttagcga atactctttg cctggaatcc agttgcgcac agaggtcttg ggatgaaact
     1381 cagacacgaa atgaagtctg tcctgttggg aaaacccaac ccagacgttt gaatcggtct
     1441 ccagtggttg atccagcgta aaccgcgcgg tcagacaact ctggccgccg gccttgagtt
     1501 tggatggaaa tctgagcagt tccatctcaa aggatggagc tgcccatgtg ggcagggcag
     1561 tcaaaaaaga gagaatcacg ccaagaagac aaagaccgtt tcgccgccac tcaggttctt
     1621 tgcgtgaaac agcaagtctt tgccttgagt tgcggtacac cagaatctcg ctggtcttgt
     1681 atttgaactt cacgagacaa cctccacatt ctcccaagaa actggactac gaaagggaca
     1741 gtttccttta catgcaagca ctactcagcg ttggacatac tcccaagtgc ctgcaggtgc
     1801 tgacccgctc atagaggaaa ctctgaaaaa gagtatcaca gttctcgtgg ttgcacaaaa
     1861 gccagagtgt tgtccgtatg tctgctcagt gatcgctgca acgggctcat aggttctgga
     1921 gatagtgttg gttctccacc catttgcatt tcggcatgtc atgatgcaag aatgcaacgt
     1981 agcattctaa acgtcaggag atacggtcat gaatcatctc aaatgcgtct gtatgctggt
     2041 gctcgtcgtt gtgtgtccca ctccagcgtt ctgccagcca gaacctctgg cagaaggcat
     2101 gatctcctgg gaagatttca gggagtttcc aattggctcc ctgattccca tcagcaacgg
     2161 tatggagaat gagtgtccga ccgttgctgt tgaccgcaag ggtaccgtgt gggtggcatg
     2221 gattcgggaa gttgctgacg gcgatcaggt ctggctgaag g
//
LOCUS       DAOQUC010000645         2260 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_59591_length_2260_cov_3.852154, whole genome shotgun sequence.
ACCESSION   DAOQUC010000645 DAOQUC010000000
VERSION     DAOQUC010000645.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2260
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59591_length_2260_cov_3.852154"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1083
                     /locus_tag="PKH07_11020"
     CDS             <1..1083
                     /locus_tag="PKH07_11020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35517.1"
                     /translation="RSRDALREARRAFCSLRLIHSMGVEQGKPGWMPKPYGFRPSVQT
                     SGDQYLYATYGLLAFHRIASSREKALIERMIIDFADYWMSVDYVLSYFGTHWDMKGET
                     DSYNAIFAMINAAAYQFSGNRIYLREVEKLMQRETWTKTTRIESLRSQVQQQMQRTGQ
                     TDVVFYGATFQLAKDLLRPGEFLCWETTVHSAFVAASAEAIHTIDPTLLDGRLPSILA
                     MWWKEWKYGVREDLMSYYWFAVDLVHDTWRPLPKTEILPRDQWLWGDPFTSYISQVRW
                     MDPLARTLITSSIAAEHCPEVSEEAEKLTDRILANLTPNHLRWLYDPDGTQLMPEIDY
                     YGKCLSSEVPSAVLGAYWARKRRARG"
     gene            1277..>2260
                     /locus_tag="PKH07_11025"
     CDS             1277..>2260
                     /locus_tag="PKH07_11025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012467011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNT35518.1"
                     /translation="MNILLVYPEFPDTFWSFKHALKFVQKKAASPPLGLLTVAALLPQ
                     EWSKRLIDLNVTKLKREELAWADYVFISGMVLQRDAVRRILRQCQEVGVKTVAGGPLF
                     TGEYEQFPEVTHFVLNEAELTLPEFLRDLEQGSPKRVYTTTEFPDIRQTPTPMWELLD
                     FSKYASMSLQFSRGCPFHCDFCNVTALFGHRCRIKTVSQILAELDRIYSLGWRDSIFF
                     VDDNFIGNKRFLKQELLPALIQWQKGKVGITFHTEASINLADDEELMNMMSNAGFDQV
                     FIGIETPDEVSLAECSKKQNRNRNLLDDVRRIQRARLQVQGGFIVGFDHDSH"
BASE COUNT          537 a          571 c          614 g          538 t
ORIGIN      
        1 cgatccaggg atgccctgcg ggaagcgcgg cgcgcatttt gttccttgcg gctcattcac
       61 agcatgggag tcgaacaagg caagcctggc tggatgccca aaccctatgg attccgaccc
      121 tccgtgcaga ccagcggcga tcagtatctc tacgccacat acgggcttct cgcatttcac
      181 cgaatcgcct cgagccgaga aaaagccctc atcgagagaa tgatcattga ttttgcagat
      241 tattggatga gcgtggacta tgtgttgtcc tatttcggca cgcattggga tatgaaaggg
      301 gaaaccgatt catacaacgc catctttgcg atgatcaatg cggcggcata tcagttctca
      361 ggaaacagga tttacctgcg ggaagtcgag aagttgatgc agcgggaaac ctggacaaag
      421 acgacacgca tcgagagtct tagaagtcag gttcagcaac agatgcagcg gacaggtcag
      481 acggatgttg ttttctatgg tgcaacattc caactggcca aggacttgct tcggcctggg
      541 gagtttcttt gttgggaaac gacagtgcat tcagcctttg ttgctgcctc agcagaggcc
      601 atccacacga ttgatcccac gcttcttgac ggccgactcc catccattct tgccatgtgg
      661 tggaaggagt ggaagtatgg agtccgagaa gatctcatgt cctattactg gtttgccgta
      721 gaccttgttc acgacacatg gcgccctctt cccaagacgg agattcttcc aagggatcaa
      781 tggctctggg gcgatccttt tacgtcctat atatcacagg ttcgctggat ggatccgctg
      841 gccagaacac tcatcacttc gagcatcgct gccgagcact gccctgaggt ttccgaagag
      901 gcagagaaac tgacagaccg gattctggca aacctgacgc cgaaccatct ccggtggctt
      961 tacgatccgg acgggacgca actgatgccg gagatagact attatggcaa gtgtctgagc
     1021 agcgaagtgc cttcggcggt tttgggagcc tattgggcaa ggaagcgtcg cgccaggggt
     1081 tgaatcctga tccggccact tgaggcaaag gcgtgtcgca catctttctc tttaggcgaa
     1141 gaggcatttt ggattgcatt tttccggcat cgtgctatag tcaccaagcg ttattagcac
     1201 tgtcaaggac accttcctca taaggtaact cttcagagca aaagtgcctt tgccagaatg
     1261 gaaggaatgc ctattcatga atatactgtt ggtttacccg gagtttccgg acacgttttg
     1321 gagcttcaag cacgcactga aattcgtgca gaagaaggcc gcatcgcccc ctttgggtct
     1381 tctgactgtg gcagcgttgc tacctcagga atggtcgaag agactcatcg atctgaacgt
     1441 gacaaagttg aagcgcgagg aacttgcctg ggcggattac gtctttatca gcggcatggt
     1501 gctgcagcgg gacgcggttc gtcgaatcct cagacagtgt caagaagttg gcgtcaagac
     1561 tgtggcaggt gggcctcttt tcaccggtga atatgagcag tttccagaag tcacccattt
     1621 cgtactcaat gaagccgaac tgacgttgcc ggagttcctc agagatctcg agcagggaag
     1681 tccgaagcgc gtctacacca cgactgaatt cccggacatc aggcagactc ccacaccgat
     1741 gtgggaactg ctggacttct ccaagtacgc ttcgatgagc cttcagtttt cgcgtggttg
     1801 cccatttcat tgcgacttct gcaatgtcac agcccttttt gggcatcgtt gccgaatcaa
     1861 aaccgtcagc cagatccttg ccgagttgga taggatttac tcgttgggat ggcgcgacag
     1921 cattttcttt gtggatgata atttcattgg caacaagcgc ttcctcaaac aggaactgct
     1981 gcccgcgctg atacagtggc agaaaggaaa ggtgggtata accttccata cggaagcgtc
     2041 catcaatctg gcagacgatg aagagctgat gaacatgatg tcgaatgcgg gttttgacca
     2101 agtcttcatc ggcattgaga cacccgacga agtcagtctc gcagaatgca gcaagaaaca
     2161 gaatcgcaac cgaaatctcc ttgatgacgt gcgccgtatc caacgggcga gacttcaggt
     2221 gcagggcggt tttatcgtcg gcttcgacca cgattcacat
//
LOCUS       DAOQUC010000646         2257 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_59726_length_2257_cov_2.777929, whole genome shotgun sequence.
ACCESSION   DAOQUC010000646 DAOQUC010000000
VERSION     DAOQUC010000646.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2257
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59726_length_2257_cov_2.777929"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2013
                     /locus_tag="PKH07_11030"
     CDS             <1..2013
                     /locus_tag="PKH07_11030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNT35519.1"
                     /translation="EHIRKDGTTFPLEVLVKPVMHGGIPAFLSIATDITERKKTEDAL
                     RKSEAKFRRVFESAALGMATVTPDGRFSEVNDVLCRILGYSRQELLQRNFREITHLDD
                     LHLDVEYIRRVLSGEIDSFVLEKRFVRSDGAAVWCILNSSVVRLQDGTPDYAVAIIQD
                     ITDRRESEAERERLLTAIEQAGETIMITDPRGFIQYVNPAFERTSGYSWQEAVGQRPN
                     ILKSGEQDEAFYAALWQTLTSGRSWEGRMVNRRKDGTLYTEDVNISPVLDSAGSIVNF
                     VAVKRDITRELEIERQYHQAQKMEAIGQLTGGVAHDFNNILQVINGATEMALTDLEPS
                     HPARFMLSEVQKAGEKAVRLVEQLLLFSRRQIMRPKTTDLNLVIEDLLKMLRRIIGEN
                     IRLEWLPGKHLGMVHADQSMLEQVVLNLCVNARDAMPQRGVLTIETQNVIMDSDYCAV
                     HTWASLGRYVLLSITDTGCGMDSELLEHIFEPFFTTKPEGKGSGLGLATVYGIVRQHE
                     GMISAYSEPDKGSTFKVYLPMCERPAEAVGVMIQGEAKGGEETILLAEDDEMVRNLAY
                     TVLSRAGYRVLVAKDGLEAVELFQRNQDDISAVVLDVVMPGLGGAEALKQMRALRPRL
                     KALFASGYSPNAIHTNFILHEGVELLQKPYGPDALLRALRVVLDSR"
BASE COUNT          530 a          542 c          651 g          534 t
ORIGIN      
        1 gagcacattc ggaaagatgg gacgactttt ccactggaag tgcttgtcaa acctgtgatg
       61 catggcggta tccctgcctt cctcagcatc gcaactgata ttactgagcg caaaaagacc
      121 gaagacgcac ttcgcaagag tgaagcgaaa ttccgccgag tttttgaatc cgcagcactc
      181 ggcatggcta cggtcactcc ggacgggcgt ttcagcgagg tcaacgatgt tctctgtcga
      241 atactggggt actcgcgtca ggaacttctt cagaggaatt tccgtgaaat aacgcatctc
      301 gatgatctac accttgatgt ggagtacatt cgaagagtgc tctcaggtga gatcgacagt
      361 tttgttctgg agaaacgctt cgttcgcagt gacggtgctg cggtctggtg cattctcaac
      421 tcatcggttg tgcgcttgca ggacggaaca cctgactatg ccgtcgccat tatccaggac
      481 atcacagatc gcagagagtc tgaagcagaa cgggaacgtc tgttgactgc gattgaacag
      541 gcgggcgaga cgatcatgat cacggacccg cggggcttca tccagtatgt caatccagca
      601 ttcgagcgca catcgggcta ttcctggcag gaagcagtcg gtcagcgtcc caatatcctc
      661 aagagtggtg agcaggacga agccttctat gcggccttgt ggcaaacgct cacgagcggg
      721 aggtcctggg aaggacggat ggtcaatcgc cgaaaggatg gcacgctcta tactgaagat
      781 gtcaacatat cacctgtact cgattcggcc ggtagtattg tcaacttcgt tgccgtcaaa
      841 cgcgatatca ccagggaatt ggagatcgag cggcagtacc atcaggcgca gaagatggaa
      901 gccattggtc agttgaccgg tggggttgcc cacgatttca acaacatctt gcaggtcatc
      961 aatggggcaa cggagatggc tttgaccgat ctggagccat cccatcctgc ccgcttcatg
     1021 ctttctgagg ttcagaaggc aggggagaag gcagtacgtc ttgtggagca gctgctcctg
     1081 ttcagccgac gccagatcat gcgacccaag acgacagatt tgaacctggt tattgaagat
     1141 cttctgaaga tgttgcgacg gatcattggt gaaaacatcc gtttggagtg gctcccaggc
     1201 aagcatcttg gaatggttca tgctgaccag tcaatgttgg aacaggttgt cttgaacctc
     1261 tgtgtcaatg cgcgggatgc catgccccaa agaggtgttc tcactatcga gacacagaat
     1321 gtcatcatgg attctgatta ctgcgctgtc cacacatggg caagccttgg acgctacgtt
     1381 ctcttgagca tcaccgacac gggctgtggg atggactctg aactgcttga gcacatcttc
     1441 gaaccgttct ttacgaccaa acctgagggg aaaggatctg gtcttgggtt ggctacggtc
     1501 tatggtatcg tccgacagca tgaggggatg atatcggcat acagcgagcc agataaaggc
     1561 tctacgttca aggtctatct tccaatgtgt gaacggcccg cagaggccgt aggcgtcatg
     1621 atccaaggtg aggccaaagg aggagaagag acgattctct tggcagagga tgatgagatg
     1681 gtacgcaatc tggcctatac cgttctcagc agagcaggat atcgggttct ggttgccaag
     1741 gatggtctgg aagccgtaga actctttcag aggaatcaag atgacataag tgccgttgtg
     1801 cttgatgttg tgatgcctgg cttgggtgga gctgaggcgc tcaagcagat gcgtgccctg
     1861 cgtcccagac tcaaagccct ttttgccagt ggctacagcc cgaacgcgat ccacaccaac
     1921 ttcattttgc acgagggagt cgaactgctc cagaagcctt acgggccgga tgctcttctg
     1981 cgggcactga gagtggtttt ggattcgcgt taattgagtt gacagtccaa cggctgattg
     2041 gtacctttcg gataaagtaa tctccttgaa tagggaagag gatcaggggc ttttacagat
     2101 gagaaagaga actgattttc gacacttctg gcgagtttcc gggtttctgt tgctcgttgc
     2161 atttcaggga tgcagtcagc gggctgcgca gttcaacacc cgtggcctcg atcttctgaa
     2221 tcggggtgag gcggccaaag cagtctccgc gtttaag
//
LOCUS       DAOQUC010000647         2256 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_59771_length_2256_cov_2.946388, whole genome shotgun sequence.
ACCESSION   DAOQUC010000647 DAOQUC010000000
VERSION     DAOQUC010000647.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2256
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59771_length_2256_cov_2.946388"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1845
                     /locus_tag="PKH07_11035"
     CDS             <1..1845
                     /locus_tag="PKH07_11035"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HNT35520.1"
                     /translation="LREKGAIPILTRTGDVNVTIGERPALCKKENAELFVSIHHNANA
                     QNDRSVNRSEVYYQWKERNAPSADFAREILQELERTFHLPSSRTYVTYAYGVLRQNET
                     TAALAEAFYIANPDAEKALRDDKTIRLEAEAIFRAIVHFYEKGRLHLKWCDEEDFAVE
                     GRSIARVVPEWGPGVDPHSICALIDGQCIAHCYDADKGLISVLPGDLPVGATHTLEIQ
                     ARNLNGISARPILRQLEGGLRLHATGDTSIPARVLRVTNHLNNPVDGAVLLLDGKAFA
                     QTGVEGTFLLPDSATENEECFEGRIKIQAPGYFTSTISLDRLRMRQSQSELPGLLMKP
                     LFGGLLQGKTFILDPEHGGEDPGVVTPDCLRASHVNLNVAQRLAAYLRTAGATVTLTR
                     TWDNTMDDEARVRFGLENEVDHFLCIDHLAFRPGENDPPDLDCTWVLQKWDWTNCQCY
                     FDSLLKELVMLLKTPKDEIRRTSTWQSMHAQHNYSVTLVSPMLMTAPRAAERLRCQGC
                     LDKEALGLLHGLARFHLETAKTEGTSVFDLSNEIWGVVVDENGTPINGVCVSLADTLW
                     MQTEPDGEFRFRFLERGAYEVRLEVPALPPRVLTLQAAAEGQIQRIQV"
     gene            complement(1982..>2256)
                     /locus_tag="PKH07_11040"
     CDS             complement(1982..>2256)
                     /locus_tag="PKH07_11040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35521.1"
                     /translation="STEPTPTPTRPVVTAIGKVVAKPQTDGNTSPGTHFLMSLDNTVR
                     LFILQSATIDLVAYEQKACVVTGNVVAVPVWGPPLMEVTGISVLTR"
BASE COUNT          545 a          561 c          601 g          549 t
ORIGIN      
        1 cttcgtgaga agggtgccat cccgattctg acccgaactg gagacgtcaa tgtcacaata
       61 ggcgaacgtc cggcactctg caagaaagag aatgcggaac tgttcgtctc gattcatcac
      121 aatgccaatg cccagaacga tcgctctgtg aaccgttcgg aagtctacta tcaatggaaa
      181 gagcgcaatg caccttcagc cgattttgcg cgagagattc tacaggaact cgagcggact
      241 tttcaccttc catcaagcag aacctatgtg acctatgcct atggagtgct caggcagaat
      301 gagacaacag cagcgcttgc cgaggcgttc tatattgcca atcccgacgc ggagaaggca
      361 cttcgcgacg acaagacaat cagactcgaa gccgaggcaa tctttcgcgc catcgttcat
      421 ttctatgaaa agggtagact gcatttgaaa tggtgtgatg aagaagattt tgccgttgaa
      481 ggacgttcga tcgctcgcgt ggttcctgag tggggacctg gagttgatcc tcatagcatc
      541 tgtgctttga ttgatggcca gtgcatcgcc cattgttacg atgcggacaa gggccttatc
      601 tctgtcttgc caggcgatct gcctgttggt gcgacacaca cgctcgagat tcaggccagg
      661 aatctcaacg gaatcagcgc tcgtccaatc ttgagacaat tagaaggtgg tctcagattg
      721 cacgcgacag gggatacttc gattcccgcc agagttcttc gagtaaccaa tcatttgaac
      781 aatcctgtgg acggagctgt tcttcttctg gacggtaaag cctttgccca gacgggagtg
      841 gaagggactt ttcttcttcc tgattcggcc actgagaatg aagagtgctt tgaaggcaga
      901 ataaagattc aagccccagg gtatttcaca tccacgattt ctctagaccg acttcgtatg
      961 cgccagtcgc aatctgagtt gcccggactt ttgatgaaac cgctgtttgg tggacttctt
     1021 cagggcaaga cattcattct tgatcccgag catggtggcg aggacccagg tgttgttaca
     1081 cccgactgtc ttcgggcgtc ccatgtgaat ctgaacgtgg cacagaggct tgctgcttat
     1141 ttgagaactg ccggggcgac cgtcacactg actcgaacct gggacaacac gatggatgac
     1201 gaagcgcgcg ttcgatttgg tctcgaaaat gaagtagacc atttcctttg catcgatcac
     1261 cttgcgttcc gaccaggaga gaacgatcca cctgatcttg actgcacttg ggttcttcag
     1321 aagtgggact ggacaaactg ccaatgctat ttcgattcgc tgctgaagga actggtgatg
     1381 ttactgaaga ctccgaaaga cgaaatacga agaacctcga catggcaaag catgcacgcc
     1441 cagcacaatt attctgtaac tctggtcagt cccatgctca tgacagcgcc aagggctgct
     1501 gagagactca ggtgccaggg ttgtctggac aaggaagcgc ttggattgct tcatggtctg
     1561 gcgcgatttc atttggaaac tgccaagaca gagggcacat ctgtgttcga cctttccaat
     1621 gagatatggg gtgtggtggt tgatgagaac gggactccta tcaacggcgt gtgtgtctct
     1681 cttgccgaca cactctggat gcagacagag ccggatgggg aatttcgctt cagatttctg
     1741 gagcgcggtg cctacgaagt gcggcttgag gttcctgctc tgcctccaag agtcctcaca
     1801 cttcaagcag cagcggaagg acagattcag cgaatccagg tatgatctct cccttctgca
     1861 ggtgctaatc tgctcagaaa caagaaacag gagttcttac tctcttttgc caaaatacga
     1921 agccctggag caaaacactc cagggcttct gggaaaactt atgtcagccc aaaaccgagg
     1981 gctatcttgt gagaacggat attcctgtga cttccatcaa tgggggtccc cagactggaa
     2041 ccgccacgac attacccgtc acaacacatg ctttctgctc atacgcaacc agatcgattg
     2101 tggcactctg aaggatgaag agtcgaactg tgttgtccag agacatcagg aaatgagttc
     2161 cgggcgatgt gttgccgtct gtctgtggtt tcgcaacgac tttcccgatg gccgtcacaa
     2221 caggtcttgt cggagttgga gttggttctg tgctgg
//
LOCUS       DAOQUC010000648         2255 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_59810_length_2255_cov_3.427273, whole genome shotgun sequence.
ACCESSION   DAOQUC010000648 DAOQUC010000000
VERSION     DAOQUC010000648.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59810_length_2255_cov_3.427273"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            36..416
                     /locus_tag="PKH07_11045"
     CDS             36..416
                     /locus_tag="PKH07_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011628418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNT35522.1"
                     /translation="MAGETILIVDDERSIQRILEFNLRKAGYETRLAEDGLQAMARMR
                     EAKPDLVVLDVMMPNMDGYEVCREIRSDTDLRDVLVVLLTARGQEIDRDKGVEVGADV
                     YATKPFSPRILLQTIRDLLDTRSQ"
     gene            444..1829
                     /locus_tag="PKH07_11050"
     CDS             444..1829
                     /locus_tag="PKH07_11050"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNT35523.1"
                     /translation="MQRISSVHSDSNAMQERQQLVQQLNEALRDLDDQLEETSSLYEQ
                     LAALYHMFGLFGQMPDTDTIPHEVLRFCLENLGATRAFVGLLDGDRLTIKYHTGLEKL
                     NTTEFLLSDEHGLLSFCRNNNRSAIVNDFSVDSRMRCSYCSSLGFKRVVCAPLSLGAK
                     VIGFLVVADTRDGSDFSAGHGKLVTSISHPLANVLENARLRQKEIEKKVLERELEIAR
                     RIQQRLLPNTPVEIPGLQICGRTVPALVIGGDYFDFFPLERERIAVVLGDAVGKGVSA
                     GLLMTMLKGMLQVLPIKQSSPGAVLEQINRALFQQDLVEHFVTMVYCVYDGSTRTLCM
                     ANAGHEPPVLFHGDGRSPEMLRLACLPLGVKPAEHYQETTITLAPEDVLLIHSDGLSE
                     ARNQQDEEFGVERIQRTVQSHLNQDAWDIVQVLHKAVERYVGDLAQHDDVTSIILRVC
                     RNAEDSKQAAS"
     gene            1866..>2255
                     /locus_tag="PKH07_11055"
     CDS             1866..>2255
                     /locus_tag="PKH07_11055"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HNT35524.1"
                     /translation="MSSTVDPIKVRQMLSRIESVPTLPTVVSELFEQLRSPRTSADDI
                     NRIIVKDQSLTSKVLRVANSAFYGFPRKISSVTHAVVILGFETVRNLALTISVFSTFS
                     STEKGGFDRLAFWRHSLACAVISRSIAA"
BASE COUNT          515 a          572 c          648 g          520 t
ORIGIN      
        1 tggcctgacg acgtacggca ggaaaggact ttgccatggc gggtgagacg atcctcatcg
       61 tagacgatga gagaagtatt cagcggattc tggagttcaa tctccgaaag gcgggatatg
      121 agactcgact cgccgaagat gggttgcagg cgatggctcg gatgagggaa gcaaaaccgg
      181 atttggtcgt actcgatgtg atgatgccga atatggacgg ctatgaagtc tgtcgagaga
      241 ttcgttctga tactgatttg agggatgttc ttgttgtctt gttgacagcg cgaggccagg
      301 agatcgatcg ggacaagggc gtggaggtgg gcgcagatgt ctatgccacc aagcccttca
      361 gtccccgaat tctccttcaa acgattcggg acttgctgga cacgcgcagt caatgatttt
      421 gttcggccgg atgaggaaaa gccatgcaga gaatcagctc ggtgcattcc gacagcaatg
      481 ccatgcagga acgccagcaa ctggtgcaac agctgaatga agcgctgcgg gatctcgatg
      541 accagttgga agagacgtcg tcgctgtatg agcagttggc ggcgctctat cacatgttcg
      601 ggctgttcgg acagatgccc gacacggaca caattcccca cgaagtcctt cgcttttgtc
      661 tcgagaacct tggggcgacg agagcctttg tcgggttgct tgatggagat cgcctgacga
      721 tcaagtacca taccggtctt gagaaactga acacgacaga attccttctc tccgatgaac
      781 acgggctgct ttccttctgc cgcaacaaca accgaagcgc tatcgtgaat gacttttccg
      841 tggacagtcg aatgcggtgt tcatattgct cgagtctggg tttcaaacgt gtggtttgtg
      901 ccccactctc cctgggagcc aaagtcattg gatttcttgt tgtagcggac accagggacg
      961 ggagcgactt ctccgccgga catggcaaac tggtcacctc aattagccat cctcttgcga
     1021 acgttctgga gaatgcccga ctgcgtcaga aagagatcga gaagaaagtc ctcgaacggg
     1081 aactggagat cgccagaaga attcagcaaa gacttctgcc aaacactccg gtcgagatac
     1141 ccggactgca aatatgtggc cggacggtgc ccgctcttgt cattgggggt gactactttg
     1201 atttctttcc cttggagcgc gaacgtatcg ctgtcgttct gggggatgcc gttggcaagg
     1261 gcgtttcggc cggactgttg atgaccatgc tcaaaggtat gcttcaggtg cttccgatca
     1321 aacagtcttc tccaggagcc gtacttgagc agatcaacag ggcacttttc cagcaggatc
     1381 tggtggagca ttttgtgacg atggtctact gtgtgtacga tggcagcact cggacgctct
     1441 gcatggccaa cgcgggacat gagccacccg tacttttcca tggggatgga cgttccccag
     1501 aaatgctccg attggcctgc ttgcctctgg gtgtgaagcc ggcggagcac tatcaggaaa
     1561 ccacgatcac actcgcgcct gaagatgttc tgctcatcca ctctgacggt ctcagtgaag
     1621 cgcgcaacca acaggatgaa gagttcggag tcgagaggat tcaacgaacc gtccagagcc
     1681 acctgaatca agacgcatgg gatatcgtcc aagtgctgca caaggctgtt gagcgatatg
     1741 ttggcgatct ggcccagcac gatgacgtga ccagcatcat ccttcgagtg tgccgaaatg
     1801 cagaagactc caagcaggcg gcttcatagg agattcactt tgccgggaaa ggaaacaatg
     1861 cacagatgag ctcgacagtt gaccccatca aagtgcgtca gatgctttca aggattgaga
     1921 gtgtccccac gttgcccacg gttgtcagcg aactttttga gcaactgcgc agcccgagaa
     1981 catccgcgga cgacatcaat cggatcattg tcaaggatca gtcgttgacg tccaaagttc
     2041 ttcgtgttgc caactccgct ttctacggct ttcccaggaa gatcagttcg gtcacgcacg
     2101 cggttgtcat tctgggtttt gaaacggtgc ggaacctggc gttgactatc tcggtgttca
     2161 gcacattcag ctcgacggaa aaaggtggtt ttgaccgact tgccttctgg cggcattccc
     2221 tcgcgtgtgc agtcatttcg cgcagcatcg ccgcg
//
LOCUS       DAOQUC010000649         2254 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_59849_length_2254_cov_4.102774, whole genome shotgun sequence.
ACCESSION   DAOQUC010000649 DAOQUC010000000
VERSION     DAOQUC010000649.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2254
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59849_length_2254_cov_4.102774"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            113..1594
                     /locus_tag="PKH07_11060"
     CDS             113..1594
                     /locus_tag="PKH07_11060"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNT35525.1"
                     /translation="MKSRYAQSLLWFIAALSVATSPAAQEGVALSDSAKSLTELQKRM
                     WNDPSFQKQFVESFLAETDIEPRVDLDERETMQEILQLIASDKLSEAEQMLLTSIGQQ
                     PDTPKQPERQSLFSKSRQEDTKPKSVSAVFDFTLANIYFQQEKLDQAIDVYRHAIGKF
                     PKFRRAWKNLALIYVRQGALDKAIPALTRVIELGGTDAVTYGLLGYGYGSTEKHLEAE
                     SAYRMAILLDPETLDWKMGLARSFFKQQRYAEAAVLCGQLLKDNPERADLWLLQANAY
                     IGLSEPLKAAENYEIVQRLGQATADVLNTLGDIYINEELYDIAAGAYVSAMEKASKTT
                     PERAIRSAKALVARGAFAETRRVVEKTEQVYGAQMTTDQRKDILKLNARLAVAEGAGS
                     AEEVRILKELVELDPLDGEALILLGQHSSRSGDVEKAEFYFERASSIEAYEADAKVRH
                     AQLLVGQGKYEQALPLLRRAQQIKPRESIQQYLEQVERVSRTR"
     gene            1810..>2254
                     /locus_tag="PKH07_11065"
     CDS             1810..>2254
                     /locus_tag="PKH07_11065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35526.1"
                     /translation="MQKRVLLILGFVCCVLSGAGQAAEVLVTEDIATSVVWTANNVYN
                     LQEQIYVLPGATLTIEEGTLVQSTAGLGGSLAVCRGAKIYVNGTKDKPVIMTSTNDTM
                     TSWHEGCNEWGNLTIMGKGLISASHYGGQPVGSNTKEPTGLNEKQM"
BASE COUNT          602 a          527 c          625 g          500 t
ORIGIN      
        1 gacccgccat taatgccgtg aaacaatgga agtttgaccc tggcacgcgc aacggagcgg
       61 ctgttcgttt ccgaatgcgt gttccgatca cgttcccgaa aggataactc gagtgaaaag
      121 ccgttatgca cagtctcttc tttggtttat cgctgctctc tctgtcgcaa cgagtcccgc
      181 cgcacaggag ggtgttgccc tctcagactc agcaaaatca ctgaccgaac tacagaagag
      241 aatgtggaac gatccgagtt tccaaaagca atttgtcgag agttttttgg cagaaaccga
      301 tatcgaacct cgcgttgatc tggatgagcg ggagacgatg caggaaatac tccaactgat
      361 tgcgtcggac aagttgagtg aagcagaaca gatgctgctg acatccatcg gtcagcaacc
      421 agatacaccg aagcaaccag aacgccagag tctgttctcc aagtcacgcc aggaagacac
      481 caagcccaaa tcggtcagcg ccgtctttga ttttaccctt gccaacatct actttcagca
      541 ggagaaactc gatcaggcga ttgatgtata tcgtcatgcg atcggaaagt ttcccaagtt
      601 ccgccgagcg tggaagaatc tcgccctcat ttatgttcgg caaggagcct tggacaaggc
      661 aatccctgca ttgacccgtg tcattgagct tgggggcacc gatgcggtca catacggttt
      721 gcttgggtat ggctatggtt cgacagaaaa gcaccttgag gcagagtccg cctaccgaat
      781 ggcgatacta ttggatccag aaaccctgga ctggaagatg ggtcttgcca gaagtttctt
      841 caagcaacag cggtacgccg aggcggccgt gctttgtgga cagttgctga aagacaatcc
      901 cgagcgcgcc gacctttggc ttcttcaggc caatgcctac atcggactct ccgaaccact
      961 caaggctgca gagaactatg aaatcgtaca gcgcttggga caggcaacag ccgatgttct
     1021 caacaccctt ggagatatct atatcaatga ggaactgtat gatatcgcag ctggcgctta
     1081 tgtaagcgct atggaaaagg cttccaagac aactccagaa cgtgccattc gctcggccaa
     1141 ggcgcttgtt gctcgcggag catttgcgga gacacgccgt gtcgtggaga agacggaaca
     1201 ggtgtatggg gcgcagatga ctaccgatca aaggaaagac attttgaaac taaacgcacg
     1261 cctggctgtg gcagaaggtg ctggcagtgc agaggaagtg cgtattctga aggaacttgt
     1321 tgaattggat ccactggatg gggaggcttt gattctcctt ggacagcata gcagccgaag
     1381 tggcgatgtt gagaaggcgg aattctactt tgaacgtgcc tccagtattg aagcatacga
     1441 agcagatgcc aaggtgcgac atgcgcagtt gttggttggc cagggaaaat acgaacaggc
     1501 gctaccgctg ctgcgccgag cacagcagat aaagccgagg gagagtatcc agcaatactt
     1561 ggagcaagtg gaacgggttt ccagaacgcg ctaaaagttc acgcgatctc aaatgacacc
     1621 tgagaatctg acttcagact atcggaattc tcactctttg ataacactct tcaaacaaaa
     1681 gatctctaac aattcaccac aacgacaaat cagaggaggt agccccaggc aaagatgaaa
     1741 ctgtccgaag ttattgtggc ttttcgctga agtttcagag gatttgttgg aaaggagtat
     1801 aggtgaaaaa tgcagaagag agtgcttttg attctgggtt ttgtgtgctg tgtgttgagc
     1861 ggagctggac aggcggcaga ggtcttggtg acagaagaca tcgccacatc cgtcgtttgg
     1921 acggccaata acgtctacaa tctgcaagag cagatttatg ttcttccagg agcgacgttg
     1981 accattgaag aaggcacttt ggtgcaaagc actgccggat tgggcggtag tctggcggtc
     2041 tgtcgtggtg caaagattta tgtgaacggg acgaaagaca agcctgtcat tatgacttcg
     2101 accaacgaca cgatgacatc gtggcacgaa ggctgtaatg aatggggcaa cctgaccatc
     2161 atggggaaag gtctgatttc ggcttcgcac tatggtgggc agccagtggg ctcaaataca
     2221 aaggagccga ccggtctcaa tgagaaacag atgg
//
LOCUS       DAOQUC010000650         2254 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_59855_length_2254_cov_3.216007, whole genome shotgun sequence.
ACCESSION   DAOQUC010000650 DAOQUC010000000
VERSION     DAOQUC010000650.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2254
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59855_length_2254_cov_3.216007"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1051)
                     /gene="glnS"
                     /locus_tag="PKH07_11070"
     CDS             complement(<1..1051)
                     /gene="glnS"
                     /locus_tag="PKH07_11070"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HNT35527.1"
                     /translation="MTASVDFVRSIILDDLKTNKWDGRVITRFPPEPNGYLHIGHAKA
                     ICLNFSIAKQFNGRCHLRFDDTNPCKEEVEYVESIIEDVRWLGWDWGEHLYFASDYFD
                     KMHQYAVQLVQAGKAYVCDLDAEAIRRTRGTLTQPGTNSPFRDRTIEENLDLFERMRQ
                     GEFPDGLRVLRARIDMASPNLNLRDPVMYRILHVPHHRTGDKWCIYPMYDWAHGLEDS
                     IEGITHSICTLEFEDHRPLYDWFLDALGVHHPQQIEFARLNLTYTVMSKRKLLELVKN
                     KFVAGWDDPRMPTISGLRRRGYTPEAVRNFCDRIGVAKADSTIDLALLEHCLREDLNK
                     RASRVMVVLNPLRVVI"
     gene            1551..>2254
                     /locus_tag="PKH07_11075"
     CDS             1551..>2254
                     /locus_tag="PKH07_11075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35528.1"
                     /translation="MKESFWQRVPVRSNLFTLVVMVCCIAAGTSSAQTTFVIGTSSWD
                     NQSELYQFVPTSFVNARVQFLITASELTSAGLTPNTVISALQWNVVTDNTPGLVEYDL
                     YMGHTSTSGFASTTFLSSSGLTNVGFITYTGTQTGWMTASFNYQSFTWNGTDNLLIQT
                     CRSGYNQGASDRIAVSSVGFSAMISGYNHVCDADAGYYTNTARPWFRLIGIAPTPTNT
                     PTSTNTPTNTPTPTNTP"
BASE COUNT          559 a          611 c          541 g          543 t
ORIGIN      
        1 caatgaccac gcgaagagga ttcagcacca ccataaccct tgaagcgcgt ttgttgagat
       61 cttcgcgaag gcaatgctcc agcagcgcaa gatcaatggt gctgtcggct ttggcaacac
      121 cgatgcgatc gcagaagttc cgaactgcct ctggggtgta gccacgtcgg cgcaaccccg
      181 aaatcgttgg cattcgggga tcgtcccaac cggcgacaaa cttgttcttc accagttcca
      241 gcaacttgcg cttgctcatg accgtatagg tcagattcag gcgggcaaac tcaatctgct
      301 gtgggtgatg gactcccaaa gcatcgagaa accagtcata gagaggccga tgatcctcga
      361 attccagagt gcagatggaa tgagtaattc cctcgatgga gtcttccaaa ccatgtgccc
      421 aatcgtacat cggatagatg caccacttgt caccggtgcg atggtgcgga acatgcagga
      481 tgcgatacat gaccgggtcg cggagattca ggttcggaga tgccatgtct attctggcgc
      541 gcaggacacg caaaccgtcg ggaaactctc cctgacgcat tcgctcgaac aggtccaggt
      601 tttcctcgat ggttcgatcg cgaaacgggg agttggtgcc aggctgtgtc aaggtgccgc
      661 gcgtccttcg aatggcctca gcgtccagat cgcagacata cgccttgcct gcctgaacca
      721 gctgaacagc gtactgatgc atcttgtcaa aatagtccga cgcgaagtac agatgctcgc
      781 cccagtccca accaagccat cggacatcct cgatgatgga ctctacgtat tcgacttctt
      841 ccttgcaggg attggtgtca tcgaaccgaa gatgacagcg accattgaac tgcttcgcga
      901 tactgaagtt caggcagatt gccttggcat gtccaatatg cagataaccg tttggctccg
      961 gtggaaaccg cgtgatgact cttccatccc atttgtttgt cttcagatcg tcgagaatga
     1021 tactgcggac aaaatccaca gaggccgtca tctctcggga ttcatctgcc gaatcatcac
     1081 cagcccgttt tttttgaatc aaatccttgt cgtccataga ctcctcttgc tgctccgaat
     1141 cgctctgcta tcgaaaagcg tctcagtgaa agtacaagac tcactatata ccaaaacagc
     1201 tgcctggacg ccaatcccgt ttgcagtggt gaggtattta cgatagccac ctgaatccac
     1261 tcattgggtc attccgaatc cttcgctcag gattgactcc gtgaggaatc tctcttctca
     1321 acagaagact actgcaatca gagagattct tcactccgcc atgctccgtt cagaatgaca
     1381 gttggaactc tacaatgtct cctacaaatc cctgagtggt ggaagacctt tgcctgggat
     1441 tttcctgttt ttgtcccata cccttatggt atatactact catcaggtgc gacacagcca
     1501 ttttggctgg cgggcgactt cccgtcaata ctggttaaag gagttgacgc atgaaagaga
     1561 gtttttggca aagagttcct gttcgatcga atctgttcac tttggtggta atggtctgct
     1621 gcatcgcagc gggaacatct tctgcccaga ccacctttgt gattggaacg agcagttggg
     1681 acaatcagag tgagctttat caatttgttc ccacgagttt cgtcaacgcc agagtgcaat
     1741 tcctcatcac agcatccgaa ctgacctcag cagggctgac tcccaacacc gtgatcagtg
     1801 cactgcaatg gaatgtcgta acagacaata caccaggatt agttgagtat gatctttaca
     1861 tgggacatac atccaccagc ggattcgcat caacgacatt tcttagcagt tcaggactga
     1921 caaacgtcgg attcattacc tatacgggga cacaaacggg ttggatgaca gcgagtttca
     1981 actaccagag tttcacatgg aatggaacgg ataatctgct gatacagacc tgtcgctcgg
     2041 gctataatca gggtgccagc gatcgaatcg ctgtttcatc cgttgggttc agcgcgatga
     2101 tatctgggta caatcatgtg tgcgatgcag atgcgggata ctataccaac accgcaagac
     2161 cctggtttcg tctcatcggc attgctccaa cgccgacaaa tactccgact tcgaccaaca
     2221 ctccaacgaa caccccaacc ccaaccaaca cccc
//
LOCUS       DAOQUC010000651         2251 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_59996_length_2251_cov_3.323770, whole genome shotgun sequence.
ACCESSION   DAOQUC010000651 DAOQUC010000000
VERSION     DAOQUC010000651.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2251
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59996_length_2251_cov_3.323770"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1372)
                     /locus_tag="PKH07_11080"
     CDS             complement(<1..1372)
                     /locus_tag="PKH07_11080"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HNT35529.1"
                     /translation="MLKHTGFVLLAVGLLLVSGAQAKPVGCIGGAGEASVAIQQLSAS
                     ELQDAFASAAKEFGVPVTVLQAVARVESNMVHAGPTCDYGYGIMHLVDNSYCQTLKEA
                     AALVGLDVEQVKSDPVSNIRAGAALIAKYAKETVGKPKSEKEYFDALKGFSGLIDDPL
                     QESQAAEYFKVMGIDVSDLREAAVSVQSDDYGPAIWNPADPSNYDTTRGGVAVDRWVN
                     HWIGVGTYAGAISWFKNPSSNVSAHFVVRASDGELTQMVRFAHRAWHCGYWNSRSIGI
                     EHEAISSNPGLWNSVPMLTVATDACRYVCNLYGFPKTRTYIVGHKEVPYASTDCPGPL
                     PWDTYMNMVNNVTSTPTPGAVIIIDDGDATYSKSGSWSYSTCSTTYAYNGDYDWIGST
                     SGASTAWAKWTPSIGTAGDYRVYVMYRNGTNRAIDAPYTVVYNGGSQTVDVNQQQNGG
                     VWVLLGT"
     gene            complement(1687..>2251)
                     /locus_tag="PKH07_11085"
     CDS             complement(1687..>2251)
                     /locus_tag="PKH07_11085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011189953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35530.1"
                     /translation="EHAKLMEAHRLRLAFMQLAVLLPLLAVATYLLFRKRGSIYYPMF
                     LAFGGACLIKVGLVIHEYFPSRYFKYLFTLALILAVGRILIYLIHVVVYPKREWLAHK
                     YREAYERFLCPICDYPIRTGPRKFLYWTRRTVHKVLPYNESTGNEEPYVCPSCGTSLF
                     EVCTSCQRVRHSLLGHCEHCGAVKTLE"
BASE COUNT          573 a          620 c          543 g          515 t
ORIGIN      
        1 aggttcccaa aagaacccac acgccaccgt tctgttgctg attgacgtcc acagtttgag
       61 agccaccgtt atagactact gtgtacggag catcgatcgc gcgattggtc ccgtttcgat
      121 acatgacgta cacccggtaa tctccagccg taccaatact tggtgtccat tttgcccatg
      181 ccgtcgaggc accactcgtc gagccgatcc agtcgtagtc accgttgtag gcataggtgg
      241 tactgcacgt tgagtaagac catgaacctg actttgaata ggttgcgtcg ccatcgtcaa
      301 tgatgatgac tgcccccggg gtgggggtgc tcgtcacatt gttgaccatg ttcatatacg
      361 tgtcccaagg aaggggaccg gggcagtcgg ttgaggcgta gggaacttcc ttatggccca
      421 caatataagt tcttgttttg ggaaacccgt agagattgca gacataccga catgcatctg
      481 ttgccaccgt aagcatcggc acactgttcc aaagaccagg atttgaactg attgcctcat
      541 gctctatgcc gatcgagcgc gaattccaat acccacagtg ccatgcccga tgggcgaagc
      601 gaaccatctg agtcaactcg ccatcactgg cacgaaccac gaaatgggcg ctgacgttgg
      661 atgatggatt cttgaaccag gaaatggcac ccgcgtaggt tcccacaccg atccagtggt
      721 tcacccatcg gtcaacggca acaccgcctc gagttgtgtc gtagttggat ggatcagcag
      781 gattccagat tgccggacca taatcatccg actggacgct cactgcagcc tctctcaaat
      841 cggaaacgtc aattcccatg actttgaagt actctgctgc ctgcgactcc tgaagcggat
      901 cgtcaatcag accgctgaat cccttcaaag cgtcaaagta ttccttttcc gacttcggct
      961 tgccaacagt ctccttggcg tatttggcga tgagagcggc gcctgccctg atgttcgaaa
     1021 cgggatcaga ctttacttgt tcgacatcca aaccgacgag cgctgccgcc tctttcaatg
     1081 tctggcagta actgttgtcc accaagtgca tgataccgta cccgtaatca cacgtcgggc
     1141 cggcatgaac catgttggac tcaacacgtg ctactgcctg tagtactgtg acgggaacac
     1201 caaattcctt tgctgcagag gcaaaggcat cctgaagttc agacgcactc agttgctgga
     1261 tcgcaaccga tgcttctccc gcccctccaa tacatcccac aggtttcgct tgggctcctg
     1321 acaccagcag aagtcccact gcgagaagaa cgaagcctgt atgctttaac atagtttcaa
     1381 cctccttcta cttctttcgc tcaaatgctc gacttatccc aatgacccta caattcaaac
     1441 caccaacttc ctcgaaatta ctctgaagcg aaccacctcc ctgcgttgcg agtattcgaa
     1501 cggctgcgcc ttcagaaagc acagaatcct cggaggcgac aaccgccatg tgtgaagtca
     1561 gagaaaagtg ttaatcgaac cgcaaagtca acaatccaaa agactacgtc aaatacattt
     1621 gtatcctaaa cagtaatatc cctgagtatc tgagtcaaga ggaatctgct gcaaccgaat
     1681 atccgattac tcgagtgtct tgaccgcccc gcaatgctcg caatgaccca gcagcgaatg
     1741 cctgacacgc tgacaggatg tgcacacttc gaacaaagag gttccacagg aaggacagac
     1801 gtaaggttcc tcatttccag ttgactcatt atagggcagg actttgtgaa ctgttcggcg
     1861 tgtccagtag aggaacttcc tgggccccgt ccgaatcgga tagtcgcaga tgggacagag
     1921 gaagcgctcg tatgcctccc gatacttatg cgcaagccat tccctcttgg ggtacacaac
     1981 aacgtggatg agatatatga gaatcctgcc cacggcaagt atcaacgcaa gagtaaagag
     2041 atacttgaag tatctgcttg gaaaatactc atggatgacc aagccgacct tgatcaagca
     2101 ggcgcccccg aacgcgagaa acatgggata gtaaatgctt cctctttttc tgaaaagcag
     2161 gtaagtggcg acagccagga gaggaaggag aaccgcaagc tgcatgaatg ccagtctgag
     2221 gcgatgcgct tccatcagtt ttgcgtgttc t
//
LOCUS       DAOQUC010000652         2248 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_60134_length_2248_cov_2.466028, whole genome shotgun sequence.
ACCESSION   DAOQUC010000652 DAOQUC010000000
VERSION     DAOQUC010000652.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60134_length_2248_cov_2.466028"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..954)
                     /locus_tag="PKH07_11090"
     CDS             complement(<1..954)
                     /locus_tag="PKH07_11090"
                     /inference="COORDINATES: protein motif:HMM:NF012509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHMP kinase"
                     /protein_id="HNT35531.1"
                     /translation="MLIRSRAPVRIDFAGGWTDVALFAKSTPGFVINAAINIYSFATI
                     HTTERRTDSSPLRHEKDLEDQHVSIYSSDFDVFVEAKDIRKLEYDGNVDLVKAAIRRM
                     NVPGGFDMITESVAPPGSGLGTSASMGVALLGLLARYAKQDLLQYEIAELASTIEREE
                     LGILGGKQDHYASSMGDISFMEFRGEDVKASRLILSRDTYLELEKNLVLCYTGKSRLS
                     GDIHKNVTEAYKSGHKDTVDAIESMKRLTLEMKCALMKGDLDAFGQLISENWRNQKRL
                     HPSVTNADIDGLFGAAMNNGAVGGKACGAGGGGCLLFYCQSD"
     gene            1113..>2248
                     /locus_tag="PKH07_11095"
     CDS             1113..>2248
                     /locus_tag="PKH07_11095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35532.1"
                     /translation="MMRWLIILFVLFLIRPVALRASDELSVTTHVAHAVRKESALDVS
                     FLLDAPAGKHGATRAEGNRLCYANGTPARFWGVNFAWEAQYPEVSQIDAIVKRLGASG
                     INLVRLTYLDHLPPKGLVSRLAPTEIVVSEERFDRLDRFATALFEAGIYVNVVLLMNG
                     KIADITPSLESNDVASLQMFHPDVIAAHERWIEFFLSHRNPYTGRTWGEEPGLAFIEI
                     YNEGDPFYLRKKIPAHSEPALAALHEKWRQWCQQESFEGSSLFDLTLLDGRLDIGREP
                     ARFLSDLQRSHLREMRAVIRRCGYTGAICDIAGGSYAPAARWAQRESDVHVAHYYHDH
                     PNWDEKGALFHNRSGCESGLRMLTAAAWERDLQKPYILGEWDFC"
BASE COUNT          493 a          626 c          592 g          537 t
ORIGIN      
        1 atccgactga caatagaaca agaggcagcc gcctccccct gcgccacaag cctttcctcc
       61 taccgcaccg ttgttcatgg cagcaccgaa aaggccatca atatccgcat ttgtcacgga
      121 tggatggagc cgtttctggt tgcgccagtt ttcactgatg agttgcccaa acgcatccag
      181 atcgcccttc atcaaagcac acttcatctc cagcgtcagc cgtttcatgg actcaatcgc
      241 gtccacagtg tctttgtgac cggatttata cgcttcggtg acgttcttgt gaatatcacc
      301 cgagagccgt gatttgcccg tgtaacacag aaccagattc ttctccagtt ccaagtaggt
      361 gtccctcgac agaatgagtc gggacgcctt cacatcttca ccccgaaact ccatgaaact
      421 gatgtccccc atcgaactgg cgtagtgatc ctgtttgcca ccgaggattc ccaactcctc
      481 gcgttcgatc gtgctcgcga gctccgcaat ttcgtattgg agcaaatcct gctttgcgta
      541 ccgtgccagc aaccccaaca acgccacccc cattgaggct gacgttccca gaccgcttcc
      601 tggcggagcc accgactcgg tgatcatgtc gaatccgccg gggacattca tccgacgaat
      661 cgccgccttg accagatcca cgttgccgtc gtactccagt ttccgaatgt cctttgcttc
      721 gacaaagaca tcgaagtcgc tggagtagat actgacatgt tgatcctcca gatccttctc
      781 gtgccgaagc gggctggagt ctgttctgcg ctcggtggtg tgaattgtgg cgaatgaata
      841 gatgttgatc gccgcattga tcacaaagcc aggagtgctt ttggcaaaca aggccacatc
      901 tgtccaccct ccggcaaagt ctattctgac tggagcacgg gaacgaataa gcatgatcat
      961 gcctccccaa ttctctgttg ttcatctgcc gactgaatga gtcgcatcac tgactctgag
     1021 attatcacaa aactgtcggc aaatagcaac ccaagccaca caaagaccac aagtgtggca
     1081 ctgtgaccca aacgctgcta agatcggagc aaatgatgag atggttgatt attctctttg
     1141 tgttgtttct gatcagaccg gtcgcgctca gagcatcgga tgaactgtcc gtgacaaccc
     1201 atgtggcaca tgccgttcgg aaggaatcgg ctttggacgt ctcttttctg ctcgatgcgc
     1261 cagcaggaaa gcatggagca acaagggcgg aaggaaaccg gctgtgctat gcaaatggaa
     1321 ctcctgcccg gttctggggt gtcaattttg catgggaagc gcagtacccg gaagtctcac
     1381 agatagacgc catcgtgaaa aggcttgggg caagtggcat caatctggtg cggctgacgt
     1441 atctcgatca cctccctccg aaaggacttg tttccagact ggcacccaca gagatcgttg
     1501 tcagcgagga gcgctttgac aggctcgacc gtttcgccac tgctctgttc gaggcgggga
     1561 tctatgtgaa tgtggtgctt ttgatgaatg gaaagattgc ggacatcacc ccatccttgg
     1621 agtcgaacga tgttgcatcg cttcagatgt ttcatccgga tgtgatcgct gctcatgagc
     1681 gatggatcga gttcttcctt tcccatcgaa atccttacac gggtcgcact tggggcgagg
     1741 agccgggctt ggcattcatt gagatctaca atgaaggcga tccgttctat ctccgcaaga
     1801 agattcccgc tcactcggag cctgcgcttg cagcgttgca tgagaaatgg cggcagtggt
     1861 gtcaacaaga gtcttttgag gggtcgtcct tgttcgatct cactctgctt gatggaaggc
     1921 ttgacatcgg acgtgaaccg gcgcgctttc tctcagatct gcaacgctcc catctcagag
     1981 agatgcgtgc ggtcattcgc cgatgtggat ataccggagc catctgtgac attgccggtg
     2041 gttcctacgc gccagcagcg cgctgggcac aacgtgagtc ggacgtgcac gtggcgcact
     2101 actaccacga tcaccccaat tgggatgaaa agggtgccct gttccacaat cgttcgggct
     2161 gtgagagcgg ccttcggatg ctgacggctg ccgcttggga gcgcgacctt cagaagccat
     2221 acattctggg ggagtgggat ttctgctg
//
LOCUS       DAOQUC010000653         2247 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_60165_length_2247_cov_3.148723, whole genome shotgun sequence.
ACCESSION   DAOQUC010000653 DAOQUC010000000
VERSION     DAOQUC010000653.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2247
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60165_length_2247_cov_3.148723"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..193)
                     /locus_tag="PKH07_11100"
     CDS             complement(<1..193)
                     /locus_tag="PKH07_11100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35533.1"
                     /translation="MSVSNGNGLNPEKNELSPEQVERTARLEAAVNKLKRIPMLSQLS
                     TDIIREIDNPNISCRRLAEI"
     gene            410..>2247
                     /locus_tag="PKH07_11105"
     CDS             410..>2247
                     /locus_tag="PKH07_11105"
                     /inference="COORDINATES: protein motif:HMM:NF019552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HNT35534.1"
                     /translation="MLYALMLLGCMVATVSEESMSAHPHLFITIQDVARARQSIRTDP
                     QLAEHAQDLLRVAREKSQENLPKLETEWWKDSQSKPWSQTYPEINYHTGKVPREWAEL
                     AWLNARASLLFPDQDLQERAMQVLLGLSEYTFDFEHYDVGMNYATWGWKCLDVYDILY
                     DRFTPDQRTELDGFFERMVQAIIKNDEFWIEKMPGGRLNNHYAWHKLARVMYGLHTNQ
                     WEMVQEALWGSKGIIDSLRYGFTDDGLWLESSLNYQLAQTSPMVMIAKLLENTDSPFS
                     LWNYQTDDGRTLKQAYDALIEIVFPDQTLPNIGDCYGSRVRLGRVVDFETLYSALGDA
                     RYPWLINQIGRRSRETLFCGVAELPEGEPPILQSRLWPEHGYAMLRSRHAVDYWTGLG
                     WTVFATYAYASVHNNADKLSIQLFANNHHWLVDREAKTGVHHAFSSAVQTELNRSTHC
                     HNTLLVDGSYQNGAARLDLIEFSSLPELKRLSIGDIKGRLYPGVQQLRTILVMEEYVL
                     DVFQAISDGTHNFDWLLHIDGQSVTSSEQSWKGDPFPETPPWKWLRNPECSDSLSCYR
                     EVFTNGKDSFSIDIATDRTAKMIRCGFPQTDHIESETWPMRMLHCT"
BASE COUNT          563 a          579 c          571 g          534 t
ORIGIN      
        1 cgatctccgc gagcctgcga caggagatgt ttggattgtc aatctcacgg atgatgtccg
       61 tactcaattg agaaagcatc ggaatccgtt tgagtttgtt gacagccgct tcaagtcttg
      121 ccgtgcgctc aacctgctca ggggacaact cgttcttctc cgggttcaaa ccatttccat
      181 tggaaacaga catcgtttcc atcctcccaa agagtgaaat acacactctt gactatttaa
      241 gttacattat cagccttaat agcaaaactc aagaaaaatc ttctaaccat attcaacaca
      301 acttgttaaa gcccgtgagc attctgccga ggcaaagatt gatatccact tgaaacggcg
      361 cttcttgtct tttatgatgg acagataaga ggcattcaag gagccacaaa tgctttacgc
      421 actcatgctt ttgggttgta tggtggcaac tgtatcggag gaatccatgt ctgcacaccc
      481 acatcttttc atcacgattc aagacgtcgc aagagcacgc cagagcatcc gcactgaccc
      541 acagttggcg gagcatgccc aggatctctt gcgtgtcgcc cgggaaaaat cccaggagaa
      601 tctgcccaag ctggagaccg aatggtggaa agattcacag tccaaaccct ggagtcagac
      661 gtaccccgag atcaactacc acactggaaa agtacctcgt gaatgggctg aactggcctg
      721 gttgaacgcc cgggcgagcc tgctgtttcc cgatcaggat cttcaggaga gagccatgca
      781 agtgctgctc gggctttcag aatacacttt cgattttgaa cactatgacg taggtatgaa
      841 ctacgccacg tggggctgga aatgccttga tgtctacgat attctctatg atcgattcac
      901 acctgaccag cgtacggagt tggatggttt ctttgaacga atggttcagg caatcatcaa
      961 gaacgatgag ttctggatcg agaagatgcc cggtgggaga ctcaacaatc actatgcctg
     1021 gcacaaactg gcgagagtca tgtacggact gcataccaac cagtgggaga tggtgcagga
     1081 agccctatgg ggaagcaaag gcatcataga ctccctgcgt tacggcttca cagacgatgg
     1141 actctggctc gaatcctctc tgaattatca gctggcacag acaagcccga tggtgatgat
     1201 tgcgaaactg cttgagaaca cagattcacc gttcagtctt tggaactacc agaccgatga
     1261 tggaagaaca ctgaagcagg cttacgatgc cctgatcgaa atcgtgtttc ctgatcagac
     1321 gctgccaaac atcggcgact gttacggcag tcgtgtgcgt ctgggtcgag tcgtggactt
     1381 cgaaactctg tactctgcat tgggagatgc tcgctaccca tggctgatca accagatcgg
     1441 ccgcagaagt cgtgagacct tgttctgtgg tgttgcagag ctgccggaag gcgagcctcc
     1501 catcttgcag tcgcgtctgt ggccagagca cggctacgcc atgcttcgaa gcagacacgc
     1561 tgtggattac tggacaggac tcggttggac ggttttcgca acctatgcct acgcatccgt
     1621 gcataacaat gccgacaaac tctcgattca gttgtttgcc aacaaccatc attggctggt
     1681 tgaccgcgag gcgaaaaccg gagtccacca cgccttctcc tcggcagttc agacggagtt
     1741 gaaccgttcg acacattgcc acaacacgct gctcgttgac ggtagttatc agaatggtgc
     1801 tgcccgactc gatctgatcg agttctcttc tcttcctgag ctgaagcgtc tcagcatagg
     1861 agacatcaaa ggaaggcttt atcccggcgt tcagcagctt cggacgatcc tggtgatgga
     1921 agagtatgtc ctggacgtgt ttcaggccat cagtgatgga acacataact ttgactggtt
     1981 gctgcacatt gatggacagt ctgttacatc gtctgaacaa tcgtggaagg gggatccttt
     2041 tccagaaacg cccccttgga aatggcttcg gaatccggaa tgttctgatt cactgagctg
     2101 ctatcgagaa gttttcacca acggcaaaga ctctttttcg atcgatatcg cgacagaccg
     2161 aacggccaag atgatccgat gcggtttccc tcagaccgac cacatcgagt cggaaacctg
     2221 gccgatgcgg atgctgcact gcaccag
//
LOCUS       DAOQUC010000654         2245 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_60238_length_2245_cov_3.689498, whole genome shotgun sequence.
ACCESSION   DAOQUC010000654 DAOQUC010000000
VERSION     DAOQUC010000654.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2245
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60238_length_2245_cov_3.689498"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(87..1004)
                     /locus_tag="PKH07_11110"
     CDS             complement(87..1004)
                     /locus_tag="PKH07_11110"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HNT35535.1"
                     /translation="MTVPYYVATRYAEPSAVSSPDGYARLARSHPQIETGQIHDPILQ
                     QRESGLVHGTPTLARTEDFLRVLDDQIGKTQPTSVSRVVKKGDNLWNLVKQELISLGR
                     QPDPKAILESISRVAQANRLSNSDLIHIGQVLDFSALNDVAETVANATVPESVILPEE
                     FALPVDGKITSHFGLREDPFNGELAFHKGMDIAAPKGTRIQASADGTVIFSGWRKGYG
                     NVVEIDHANGITSLYGHNEKNLVTVGQWVKKGSDIARVGATGRATGPHVHFELREGQK
                     AVDPIAFVSRGVRFANTITSQRERRFPSV"
     gene            complement(1236..>2245)
                     /locus_tag="PKH07_11115"
     CDS             complement(1236..>2245)
                     /locus_tag="PKH07_11115"
                     /inference="COORDINATES: protein motif:HMM:NF036826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HNT35536.1"
                     /translation="GNSWTEGPFVIKHKETYYLMYSGNFYGSSAYAIGYATASHPLGP
                     WTKHQGNPILKQTDRVSGPGHNSVVASPDGTEFFIVYHTHQSFAGGGARQLAIDRLRF
                     VEKQESLDELEVVAGPSVLTQPLPSGALPFPAGKSDDFDGETLDRSLWTVFNEDLTKW
                     RLEDGKLIIRTQKGMVHTTNANLRNLFLQYAPEGDFLFEVRLNYSPVSLYDHAFLMVW
                     HDHNNYIQFSKMRGGSGEVYVCGIERNGEFSSYSAAKGTDALCHLRIIRRGTRIEFFG
                     SDDGSNWDRIGNGYNATSPQAKIGFGAASPYSGSERDVTFEWFKVLGQKDLKPVNMML
                     R"
BASE COUNT          507 a          576 c          579 g          583 t
ORIGIN      
        1 acagatggcc agtcagccgg tggttttcgc gtcgctgttg atcgccgccg cgcccacgct
       61 tctgctgttc gtcttctgtc agaatatcat actgagggga atcgtcgttc ccgttgagaa
      121 gtgattgtat tggcaaatct gacgccgcga gagacaaacg cgatgggatc tacggccttc
      181 tggccttctc ttaattcgaa gtgaacgtga ggccccgtgg cacggccggt tgctccgact
      241 cttgcaatat ctgacccttt cttcacccac tgtccaacag tgaccagatt cttctcattg
      301 tgaccgtaga gtgacgtgat gccattggcg tgatcgatct ccaccacgtt gccatagcct
      361 ttccgccagc cactgaagat gacagtgcca tcggcagaag cctgtatccg ggttcctttg
      421 ggtgcggcaa tatccattcc tttgtggaat gcgagttcac cgttgaacgg atcctctctc
      481 agcccaaaat gtgatgtgat cttcccgtca accggcagag caaattcctc cggcaaaatc
      541 accgattcag gaacagtggc gtttgccaca gtctccgcca cgtcgttcag ggcagagaaa
      601 tccagaacct gtccgatatg aatcaggtcg gagttactca atctgttcgc ctgtgccact
      661 cgggaaatgc tttccagaat ggccttcggg tcgggctgcc tgcccaaaga gatcaactcc
      721 tgcttgacca gattccagag gttgtcaccc ttctttacga cccgactcac gcttgttggt
      781 tgagtcttgc caatctggtc gtccaaaacg cgaaggaagt cttctgtcct tgcgagcgtg
      841 ggcgtgccgt ggactaaacc ggactctcgt tgctgaagga ttggatcgtg aatctgtccg
      901 gtttcaatct gtgggtgaga ccgagcaagg cgagcatagc catcgggtga gctcactgca
      961 gacggctctg cgtatcgagt ggcaacatag tatggtacag tcaatcctcg ctgcatttca
     1021 cgaaatactg gtcgcagtaa tcaggccaac agacccaaag ggattctgat ttgtgtgttg
     1081 tttgtgtaaa tgctgtaata tcaatatgtt atattcaaat catggtgacc ccatggcaag
     1141 aataagctga gaacggttac ctattggctc tcaacgatgg gaattttctt cccataggat
     1201 ggttgagtag gcaatgatgt tcttttgcag gctccctatc gtaacatcat gttcaccggt
     1261 ttgaggtctt tctgtccaag tactttgaac cactcaaaag ttacatctct ttcgcttcca
     1321 ctataggggg aggcagcccc gaaaccgatt ttcgcttgag gggatgtagc attgtaccca
     1381 tttccaattc tgtcccaatt ggaaccatca tcgcttccga aaaactcgat gcgggtacct
     1441 cgtcggatga tccgcaggtg gcagagagca tctgtcccct tggcggcgga gtaggaagaa
     1501 aactcaccgt ttctttcaat tccacagacg tagacctctc ccgagcctcc cctcattttc
     1561 gagaactgta tgtagttgtt gtgatcatgc cagaccatga gaaaggcgtg atcatagagg
     1621 gaaactgggc tgtagttgag acgtacttcg aaaagaaaat ccccctcagg agcatactga
     1681 agaaagaggt tccggagatt tgcgttggtg gtatggacca tgcctttttg ggttcggatg
     1741 atgagtttcc cgtcttcgag tctccatttg gtgagatcct cgttgaagac agtccagaga
     1801 gatcgatcca gtgtctcacc gtcgaagtcg tcgctcttcc ctgccggaaa tgggagagca
     1861 cccgaaggca aaggttgcgt caacacagac gggccagcga ccacttccag ctcgtctagg
     1921 gattcctgct tttcaacaaa ccgaagtcgg tcaatcgcca attgccttgc gccgcctcca
     1981 gcgaaactct ggtgcgtatg gtagacaatg aaaaactccg ttccatcggg ggaagcaacc
     2041 acggagttat gtccgggtcc ggacactcga tctgtttgct tcaaaatggg attaccctga
     2101 tgtttggtcc atggaccgag aggatgggac gctgtggcgt atccaattgc ataggcactg
     2161 cttccataga agttccctga atacatgagg tagtaggtct ctttgtgctt gatgacaaag
     2221 ggtccttccg tccaggagtt tcctt
//
LOCUS       DAOQUC010000655         2242 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_60386_length_2242_cov_2.802926, whole genome shotgun sequence.
ACCESSION   DAOQUC010000655 DAOQUC010000000
VERSION     DAOQUC010000655.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2242
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60386_length_2242_cov_2.802926"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..704)
                     /locus_tag="PKH07_11120"
     CDS             complement(<1..704)
                     /locus_tag="PKH07_11120"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNT35537.1"
                     /translation="MQYREYGKGGPTVSRLGFGVMRLPARRKGDWGSVNFSRSCKVMR
                     LAMEQGVNFFDSHHLYHHGLSEVAIGKALKGWKGQRIYLQTKTPFYNEEPIDYFKKLL
                     DEALEKMGVNCIDYLLFHSVRLDTFKKRHKQFFKFTDWAMKRGLILHRGFSSHDSPEN
                     IKTFVDTGEFSCMLVSYNWLSPAVADVIGHAADKGMGVSIMNPVGGGTLSVTTPQILR
                     LLPGAKSGTEVALRYVL"
     gene            813..1985
                     /locus_tag="PKH07_11125"
     CDS             813..1985
                     /locus_tag="PKH07_11125"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNT35538.1"
                     /translation="MTDTQHHGIIRSVFDVNSDLEPRLSLMGIPFLFPQRFLDLRLDR
                     LGRCNLRCKMCYYRYQEQPYPVLPVEKWHWIAREFFPRSRSVGFGNCAEPLLDPALPE
                     LLGKAVEYRVPEIRFITNATLLDEEKARLLVNSRVFSVTISMDAASEPLFSELRCGAK
                     LNQVITNLETLVQVKKSLGSNFPKIQFNFVIMKSNLSHLPDFIRLVTRFKPSEINAYF
                     AHLETGCPHEEEIIDATHPDFPAVADEAAALAYEAGLQLHLPYKASGSTTRTSSSLTL
                     KRLIWSMKRISSKLKNHPPSASLSAFRMCTHFAMFEKGSLCWYPNFMLMIQPDLRMIP
                     CCSWGTDPMGPIEEGMTFEEIWNLPEMSSLRGSLRTRQNIPPNCVNCMEATSRKVL"
BASE COUNT          533 a          609 c          537 g          563 t
ORIGIN      
        1 agaacgtagc gcagggcaac ctcggtgccc gattttgcac cgggaagcaa ccggagaatc
       61 tgcggtgttg tgaccgaaag agttccacct ccgaccggat tcattatgga aacacccatg
      121 cccttgtccg ccgcgtgtcc gataacgtcg gcaaccgctg gactgagcca gttgtagctg
      181 accaacatac aggaaaattc cccggtatca acgaaggtct tgatgttctc cggtgagtca
      241 tgcgaggaga atccgcgatg cagaatcaat ccccgtttca tcgcccagtc ggtaaacttg
      301 aaaaactgct tgtggcgttt cttgaatgtg tccagtctca cggagtgaaa cagcagatag
      361 tcaatacagt tcacacccat cttctcgagt gcttcatcca gaagcttctt gaagtagtca
      421 atgggttctt cattatagaa aggcgtcttg gtctgaaggt agatgcgctg gcctttccag
      481 cccttgagtg cctttccgat ggcgacttcg ctcaagccat gatggtagag gtgatgactg
      541 tcaaagaagt tcacaccctg ttccatggcc agcctcatga ctttgcagct tcgactgaaa
      601 ttcacgctgc cccagtcccc ttttcgtctt gccggaagcc tcatcactcc aaatccgagc
      661 cgcgatacgg ttggtcctcc cttgccatat tcccgatatt gcatcaaaca gatctccttg
      721 cagaaaagtt catagcgctg cattccatgc tgctataaaa tagtcttatc tcaaaccctg
      781 tcaagacata cacctgccct gtcgggtgcg tcttgactga cacccagcat catggcatca
      841 tccgaagcgt ctttgatgtg aattcggatc tggaaccgag gttgagtctc atgggtatcc
      901 cttttctgtt tccgcagaga tttctggatc tccgtcttga tcgactcgga cgatgcaatc
      961 tgcgctgcaa gatgtgctac taccgatacc aggaacaacc ctatccagtc ttgcctgtcg
     1021 agaagtggca ctggatcgca agagagttct tcccccgaag tcgttcagtg ggttttggca
     1081 actgcgccga accgcttctt gaccctgcac tgccggaact ccttgggaaa gcggtggagt
     1141 atcgtgtgcc cgaaatccga ttcatcacca atgccaccct gctggacgag gaaaaggctc
     1201 gattgctcgt caacagtagg gtcttttccg tgacgatttc tatggatgct gcgtcagagc
     1261 ctttgttcag cgagcttcgt tgcggggcaa agctgaacca ggtgatcaca aaccttgaga
     1321 cgctggtgca ggtcaagaaa agccttggct caaactttcc gaagattcag ttcaattttg
     1381 tgatcatgaa aagcaacctt tctcatctgc cagatttcat ccgattggtc acccgtttca
     1441 aaccctcgga gattaatgca tattttgcac acctggagac tggttgcccc catgaggaag
     1501 agatcattga cgcaacacat cccgattttc cggctgtcgc tgacgaggcg gcagcactgg
     1561 cctatgaggc gggtctgcaa ctgcacttac cctacaaggc gagcggttca actacccgca
     1621 cgtcatcgtc cttgacactg aagcgcctca tctggagcat gaaaagaata tcatcaaagc
     1681 tgaagaacca tcccccttct gcctcactct ctgcattccg aatgtgtact cacttcgcga
     1741 tgttcgagaa aggttctttg tgctggtatc cgaatttcat gctcatgata cagcctgatc
     1801 tccgcatgat tccttgttgt tcttggggga ctgacccgat gggaccgatt gaggaaggaa
     1861 tgaccttcga agagatctgg aaccttcccg agatgtcttc gctacgagga tcactcagaa
     1921 ctcgccagaa catccctcca aactgcgtga actgtatgga agccacttcc cgcaaggttc
     1981 tctagcgaaa cgcgttggca agttctgtgg tacgctgaaa cagaaaagcc agcctcttac
     2041 gagtctggct ttttccatct aactgttcct taacaatcga gcagaacgac gtgtgacggg
     2101 tcacagatct gtcgaaacag atcattgacc atgggagact atggtgagca aagggacata
     2161 caccgtataa tcccaagtct gctcactgtt tctccttaga aaggaggtga tccagccgca
     2221 ccttccgata cggctacctt gt
//
LOCUS       DAOQUC010000656         2239 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_60511_length_2239_cov_2.957418, whole genome shotgun sequence.
ACCESSION   DAOQUC010000656 DAOQUC010000000
VERSION     DAOQUC010000656.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2239
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60511_length_2239_cov_2.957418"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1178
                     /locus_tag="PKH07_11130"
     CDS             <1..1178
                     /locus_tag="PKH07_11130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35539.1"
                     /translation="VGLPLLPQHLSREEYLRRVAQQALETLEGTSVCPSLLIEPTACQ
                     LSEANPRNLPVHAVLFEWFPHFTNEFYSLAVDAFSSDLDHSGKPRRLVVAAHESWLTE
                     DGRWRRGLWWTGLEDDLLIESDIEPSLSPLCVQTICAPPEAHSRIHDWCVRHSISQVN
                     PYPISAVADDKYECFLRWKESGVPTPETRLIQRGTPKQKTESLLAEWISQTARDNPDC
                     CRDTALFIQPNLGTEGRDVCVYTVPSGFQDAVQRITAMAVTEPVIVRPSCGNIYWRDD
                     SSGAIISCVLRVNVGFDGTEHRAESGYLQVASSPGESVVSAGRGGHVVEFRKGALESL
                     VTAEGKSVVLDAESIRTLKMIAANAAQTIGGVGLFGVDLRLEVTASPQAPRSSMLLS"
     gene            1352..>2239
                     /locus_tag="PKH07_11135"
     CDS             1352..>2239
                     /locus_tag="PKH07_11135"
                     /inference="COORDINATES: protein motif:HMM:NF012509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHMP kinase"
                     /protein_id="HNT35540.1"
                     /translation="MEGIAYARAGLLGNPSDGYFGKTLSMIVKNFRARVTCEPSDEIV
                     IVPAPEDEPRYPSLAAMREKFQLHGYYGGVRVLKASIKRFSDYCIEQGITLPEKGFRL
                     TYESNIPRLVGMSGSSAIVTATFRALMGFFNIQIPKQILPNWTITTETQELKIPGGLQ
                     DRVVQSYEGVVYMDFSKALIQSRGYGEYEPIDPKLLPPIYLAYMTELGEGSEVFHSNL
                     RERFDRGDEAVVSAMQKFAGFAEQGRKALLDGDHSRFSKLMNDNFDLRASLYTLGKMH
                     HEMIGRARAVGASAKFAGSG"
BASE COUNT          483 a          570 c          658 g          528 t
ORIGIN      
        1 aggtgggact gcctttgctg ccacaacatc tgtcgcgaga agagtatctc cgaagagtgg
       61 cgcagcaggc gcttgaaact ctggaaggga cttctgtctg tccgagtctt ctgatcgaac
      121 cgaccgcctg ccaactcagt gaggccaatc ccaggaacct tcccgttcat gccgtcttgt
      181 ttgagtggtt tccccacttc accaacgagt tttactcgct tgcggtggac gcgttttcct
      241 cagatctcga tcactctgga aagcccagac gccttgtcgt tgcagcgcat gagtcctggt
      301 tgaccgagga cggtcgttgg cggcggggac tgtggtggac ggggttggaa gatgatctgc
      361 tcatcgagtc ggacattgag ccgagccttt ccccgctctg cgttcagacg atttgcgctc
      421 cgcccgaagc gcattcgcga attcatgact ggtgcgttcg acattcaatc tctcaggtga
      481 atccatatcc gatttctgcc gttgcagatg acaagtatga gtgctttttg cgctggaaag
      541 agtcgggtgt gccaacaccc gagacacggc tgatccaaag aggaacgccg aagcagaaga
      601 cggaatcgct cctggcagaa tggatctcac aaactgcgcg tgacaatcct gactgttgtc
      661 gggatacggc gctcttcata cagccaaatt tgggaacaga gggaagagac gtctgcgtgt
      721 ataccgttcc gtcgggattt caggatgcgg ttcaacgcat cacggcgatg gccgtcactg
      781 agccggtcat cgttcgtccg agctgtggaa acatctattg gagagacgac tcatctggtg
      841 cgatcatctc gtgtgttcta cgtgtgaacg ttggcttcga cggaaccgag cacagagctg
      901 agagtggcta cctccaggtt gcctcgtcac ctggtgagtc agttgtgtca gccggacgtg
      961 gcggacatgt agttgaattt cgcaagggtg cgcttgagag ccttgttaca gcggagggga
     1021 agtcggtggt gctggacgcc gagagcatcc gcaccctgaa gatgattgca gccaatgcgg
     1081 ctcaaaccat tggcggcgtt ggattgttcg gtgtcgatct gagactcgag gtgacggcaa
     1141 gcccgcaggc accccgatcc tcaatgcttt tgtcatagac atcaacccac gccccgctgg
     1201 tctgatgcac tcggacagtc tgccaaaagg gacagaggga atggaagccg ttcagaaatc
     1261 cgctcgtgaa ctctcatact tgccttgcat ttcaccgtca atgtggttag catcgctgct
     1321 gttgtcacaa tgatgggagg actctttcga gatggaagga attgcttatg cccgagctgg
     1381 gttgctcggc aatccctctg atgggtattt tgggaagacg ctctcaatga tcgtcaagaa
     1441 ctttcgtgca cgtgttacgt gtgagccttc tgacgagatt gtaatcgtgc ctgcgcctga
     1501 ggatgagcca aggtatcctt cgcttgccgc gatgcgagag aaattccagc tgcacggtta
     1561 ctacggaggc gttcgggttc tgaaagcgtc catcaaacgc ttctcggatt actgcatcga
     1621 gcaggggatc acccttcctg agaagggctt ccggctcact tacgagagca acattccccg
     1681 gctggttgga atgtccggct cgagtgccat tgtgacggcg acatttcgag cactcatggg
     1741 attcttcaac atccagatcc ccaaacagat tctgcccaac tggacgatca cgacggagac
     1801 acaggagctg aaaattcctg gtggacttca ggatcgggtg gtgcagtcct atgaaggcgt
     1861 ggtctatatg gacttttcaa aggcgctgat acaatccaga ggctacggtg aatacgagcc
     1921 gatcgatccc aaactcctgc caccgatcta cttggcgtac atgaccgagc tgggagaggg
     1981 gtcggaggtc ttccacagca atctgaggga gcgttttgat cgaggagatg aggcggttgt
     2041 ttcagcgatg cagaaatttg caggttttgc ggagcaaggc agaaaggcat tgctggacgg
     2101 agatcacagt cggttctcaa aactcatgaa tgacaatttc gatctgcgtg cttcgctcta
     2161 cacgcttggc aagatgcacc atgagatgat tgggcgcgcg cgcgctgttg gagcttccgc
     2221 gaagtttgct ggctccggt
//
LOCUS       DAOQUC010000657         2238 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_60556_length_2238_cov_2.709574, whole genome shotgun sequence.
ACCESSION   DAOQUC010000657 DAOQUC010000000
VERSION     DAOQUC010000657.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2238)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2238)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2238
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60556_length_2238_cov_2.709574"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1038)
                     /locus_tag="PKH07_11140"
     CDS             complement(<1..1038)
                     /locus_tag="PKH07_11140"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNT35541.1"
                     /translation="MKVVLVAPPSEKGKYEHGNQSLPKIGLGYLASMLEAKGIPCEII
                     DAKFERLSLDEVLSRLNALKPDLVGLTAMTPDICDAALVARQAKGQNPKCWTIVGGPH
                     AMALPRETMEEFHEFDLLIAAEGEYALVDVVRALEENRAMENIAGVAFRKDGQVYVNP
                     VGPWIDNLDSLPFPAWSKYPSHSDVYYVLAVRGCPYRCVFCMRSMGKTPRRRSPENVV
                     QEIQWLVQQHHAKEFIFIDETFGLNREHVAQMLALLTDRGLHRTIRWTAQTRVDRVDE
                     DLLRSTRTAGCYRVEFGIESGNQKILDAVQKGTTLQQAEYAVRLAKKSGLQVGCSFIL
                     GHPHETRETAQD"
     gene            complement(1035..>2238)
                     /locus_tag="PKH07_11145"
     CDS             complement(1035..>2238)
                     /locus_tag="PKH07_11145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35542.1"
                     /translation="LSGLCIGLAVATKYSGAALVVLLGVAHLMRCRSDVRLSQVLNLR
                     LLAAASMILLGFVLGCPFSILDFPTFIHDLSWQFTRVHSGSFGMDVDSAWHYYFTEAF
                     PYSIGYGLTVLSVIAFLFSVFRHTREDILAGSFVFCYALYVGSWKVAIHKYMLPVLPL
                     MLLLSARFIVEMSDQMRHPKRAALFVLVLSLVFAAEPFARSVRGNYLLCQKDTRTVAK
                     EWVENNIPHNTTIAVDAGNFDLAKFSPPLNDSEESLRKKRERLLTEMPETWAAARDKI
                     VRYLDIQLKYAQRGGYQLTHIVHNSDTDIDTNVSVERFRADGIRYVIASSYASQVYLD
                     PVFRASSPGKAQYYSAYYTSLERECRLIQAFYPVSAKEGPGPIIKIYEVPASSESQDG
                     PIEGRDEA"
BASE COUNT          549 a          614 c          556 g          519 t
ORIGIN      
        1 atcctgagct gtctctcgtg tctcatgggg atggcccagg atgaaactgc atcccacctg
       61 aagtccggac ttcttagcca gacgaacggc atactcagcc tgctgtaacg tcgttccctt
      121 ttggacagca tccaagattt tctgatttcc tgactcgatg ccgaactcca ctctatagca
      181 gccggcggtt ctcgtggatc ggaggagatc ctcgtccacg cgatcaacgc gagtctgagc
      241 tgtccatcga attgttctat ggagtcccct gtcagtgaga agtgccagca tctgtgcgac
      301 atgctctcga ttcaggccga acgtctcatc aataaagatg aactccttgg catggtgctg
      361 ttgcaccagc cactgtatct cctgtacgac attctcgggc gaccgccttc gaggagtctt
      421 tcccatgctt ctcatacaga acacacaacg atatggacat ccacggacag caagcacata
      481 atagacatca gaatgcgatg gatatttaga ccatgccgga aagggaagtg agtcgagatt
      541 gtcgatccac ggaccgactg gattcacgta tacctgccca tcctttctga aagcaactcc
      601 ggcgatgttc tccattgcgc gattctcttc cagggcacgt accacatcaa ccaacgcata
      661 ctccccctct gcagcgatca ggagatcgaa ctcatgaaac tcctccatgg tttctcttgg
      721 gagcgccatg gcatggggac cacccacaat ggtccagcat ttcggattct gcccctttgc
      781 ttgacgcgct acgagtgccg cgtcacagat gtccggtgtc atcgccgtca gtccgacgag
      841 atccggcttc aaggcattca gcctggaaag cacttcgtca agagagagtc tctcgaattt
      901 cgcatcaatg atctcgcacg gaatcccttt cgcttcgagc atggaggcaa ggtaacccaa
      961 accgattttt ggaagggact gatttccgtg ttcatacttc cccttctcgg atgggggcgc
     1021 taccaatact actttcatgc ttcatctctc ccttcgattg ggccatcctg agattcactg
     1081 ctggcaggta cctcatagat cttgatgatg ggacccggcc cctcctttgc tgacacagga
     1141 tagaatgcct gaatcagccg acattcacgc tccagggatg tgtaataggc gctgtagtat
     1201 tgtgcctttc caggtgagct ggccctgaag actggatcaa gatagacttg ggatgcataa
     1261 ctgctggcga tgacatagcg gattccatct gctcggaatc tctcaacgct gacgttggtg
     1321 tcaatgtccg tgtcgctgtt gtggacgatg tgtgtcagtt gatagccacc cctttgagca
     1381 tacttcaact ggatgtcaag gtacctcacg atcttgtctc tggcagcagc ccaggtttcg
     1441 ggcatttcgg tgagaagtcg ttcccgcttc tttctgaggc tctcctcgga atcattgagc
     1501 gggggactga acttggccag atcaaagttc cccgcatcaa cggcgattgt ggtgttgtga
     1561 ggtatattgt tctcaaccca ttctttggca accgttcgag tgtccttctg gcacaggaga
     1621 tagtttcctc gaacactcct tgcgaagggc tctgcagcga agaccagcga gagcacaaga
     1681 acaaagagag cagcacgctt tggatgcctc atctggtcag acatctcgac aatgaacctt
     1741 gcggacaaca gaagcatcag tggcaacaca ggcaacatgt acttgtgaat tgccactttc
     1801 caacttccta catagagggc gtaacaaaac acaaaagaac cagccaggat atcctctcgg
     1861 gtatgcctga acacgctgaa cagaaaagca ataacactta acacggtcag gccatagcct
     1921 atcgaatagg gaaaggcttc ggtgaagtaa tagtgccatg cgctgtcaac atccatgcca
     1981 aaactccccg agtgtacccg agtgaactgc caggaaagat catggatgaa cgttggaaaa
     2041 tcaaggatgc tgaaagggca tccaagcaca aaaccgagca ggatcatcga agcggctgcc
     2101 aggagacgca gattcagcac ttggctcagc cggacatcac tcctgcaccg catcagatgc
     2161 gcgacaccaa gaaggacaac cagtgcagca ccgctgtatt tggttgccac ggcaagacca
     2221 atgcagagtc cggagagg
//
LOCUS       DAOQUC010000658         2234 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_60710_length_2234_cov_3.030748, whole genome shotgun sequence.
ACCESSION   DAOQUC010000658 DAOQUC010000000
VERSION     DAOQUC010000658.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2234)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2234)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2234
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60710_length_2234_cov_3.030748"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(99..1025)
                     /gene="miaA"
                     /locus_tag="PKH07_11150"
     CDS             complement(99..1025)
                     /gene="miaA"
                     /locus_tag="PKH07_11150"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HNT35543.1"
                     /translation="MIILTGATASGKSSLSLPLAERLGAEIACMDSMQVYRGMDVGTA
                     KPTAQDRARVPHHLLDLVAPTENFSTASWLSCAEKVIRSCAERGKRLLFCGGTGLYMK
                     ALLDGLFHAPRVDPALRDELIQEGTRLGAAFLHRDLSEVDPEAAEAIHPNDLRRIVRA
                     LEVYRQTGVPISELRKGAGTSALRGRSIVFCIHREKPDLEQRIADRTRWMFENGLVEE
                     ARHLLDAGCTQSNTAMQGLGYRECVEHLKGRITREESEALVTQNTRRYAKRQGTWFRK
                     EHDTIWVFWQPDERLDSVLDRVLERVLRVGSV"
     gene            complement(1022..>2234)
                     /locus_tag="PKH07_11155"
     CDS             complement(1022..>2234)
                     /locus_tag="PKH07_11155"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNT35544.1"
                     /translation="SGKTVLAAHIIGRLRRPTLFFVHTRDLLYQTRNMLEGVLGIPIG
                     QIGDGTVDIRDVTVGTIQTCSRALGLSQARDAEGESSDSELEEESPGEEPELLPERVE
                     QITQLLRTVSVAIFDECHHIPAETSYLLALNLPKAVFRFGLSATPYRSDRQDMLIEAA
                     LGERLISINASYLIERQHLVPPHITFFVMQGGEGNQGRTYHEVYQREIVENDKRNQFA
                     AEIARKFLSQNKTVLILVGQIKHGARLASLLPEAVFLTGRDSSTVRNEALDGLRDRTK
                     PLIIATTLADEGLDIPSLDVLILAGGGRSETRALQRIGRALRPAKDKTKAYIVDFFDE
                     ARFLSEHSKSRIEIYETERYFQMEMINPWTGKNLSLEEFSSGSSRTPQQTSLLPDTPK
                     KRRARRPQPSL"
BASE COUNT          469 a          645 c          591 g          529 t
ORIGIN      
        1 tattacgtgt gatggacgtt tcagataacc gtactggcac attttttcgg gtttttacct
       61 gacctgccag atccgacgga tcagtctggt cgagtctatc aaacagatcc aaccctcaga
      121 accctctcca aaaccctatc cagaaccgag tcaagtctct cgtccggctg ccagaaaacc
      181 cagatcgtgt catgttcctt tctgaaccag gttccctgtc gtttggcgta ccgtcgggtg
      241 ttctgagtca ccagtgcttc cgattcttcc cgtgtgatcc tgcccttaag gtgttcgacg
      301 cactcccgat atcccaagcc ctgcatggcc gtgttgcttt gcgtgcatcc agcatcaagg
      361 agatgtcttg cctcttccac caggccattt tcgaacatcc accgcgttct gtccgcgata
      421 cgttgctcga gatccggttt ctcgcggtgg atacagaaca cgatagaccg cccccgcaag
      481 gcggatgtgc cagcaccctt tcggagttcc gaaatcggca cacctgtttg ccggtacact
      541 tccagagccc tcacgatgcg tcgaagatcg tttggatgga tcgcctcggc ggcttccgga
      601 tccacttcgg acagatcacg atggagaaag gcagcgccga gtcttgttcc ctcctggatc
      661 agttcatcgc gcaaagcagg atcgactcgt ggcgcatgaa agagaccgtc cagaagggct
      721 ttcatataaa gacccgttcc gccgcagaag agcaggcgtt tcccgcgctc ggcgcacgaa
      781 cgaatgacct tttctgcaca ggagagccac gatgcggtcg agaagttctc tgttggcgcg
      841 accaagtcca gtagatgatg aggtactcgt gcccggtctt gcgctgtggg ttttgccgtt
      901 ccgacatcca ttccacggta gacttgcatg gaatccatgc acgcgatttc cgcaccaagg
      961 cgttccgcca acggcaatga aagggagctc ttgccgctgg ccgtggctcc ggtgaggata
     1021 atcaaaggct gggttgcggt cgtcgtgcgc gcctcttctt gggagtgtct gggaggagac
     1081 ttgtctgctg cggcgtccgt gacgagccac ttgagaactc ctccagactc aggttctttc
     1141 ccgtccacgg gttgatcatc tccatctgga aatagcgctc agtctcatat atctcgatgc
     1201 ggctcttgct gtgctcggac aggaagcgag cctcatcaaa gaaatccaca atgtaggcct
     1261 tggtcttatc tttggcaggt cgcaaggcac gaccgatacg ttgcagtgcc cgtgtctctg
     1321 atcgtccacc acctgccaga atcagcacat cgaggcttgg gatatccaaa ccttcatctg
     1381 ccagggtcgt ggcaatgatc agcggcttcg tgcggtctct cagtccgtcg agggcttcat
     1441 tgcgaaccgt gctggaatcc cttccggtca gaaaaacggc ctctggaaga agagaggcaa
     1501 gtcgcgcccc atgcttaatc tgtcccacca ggatcaagac ggtcttgttc tgcgaaagga
     1561 acttgcgggc gatctctgct gcaaattggt tccgtttgtc attttcgaca atttcacgct
     1621 gatagacttc gtgatacgtc ctgccctgat tcccctctcc gccctgcatt acgaagaagg
     1681 ttatgtgcgg gggcaccaga tgctggcgct caatcagata ggaggcgttg atcgagatca
     1741 gacgctcccc aagcgccgct tcgataagca tgtcctggcg atcggaacga tagggggtgg
     1801 cgctcaagcc gaatcgaaac acggcctttg gcagattcag cgccagaaga taggaagtct
     1861 ctgccgggat atgatggcat tcatcaaaga tagcgacgga aacggtcctc agaagctgag
     1921 tgatctgttc aaccctttct ggaaggagct ccggttcttc ccccggagat tcctcttcca
     1981 attcgctgtc agatgattca ccttcggcgt ctcgcgcctg gctcaaaccc aatgctctgg
     2041 aacaggtctg aatggttccg acagtgacat cccgaatatc taccgttcca tcaccgatct
     2101 ggccgattgg aatccccagt accccttcca gcatattgcg ggtttgatag agcaagtccc
     2161 gtgtgtggac gaaaaacagc gttggccgtc tcaggcgacc aatgatatgc gccgccaaaa
     2221 cagtctttcc ggag
//
LOCUS       DAOQUC010000659         2232 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_60796_length_2232_cov_3.469913, whole genome shotgun sequence.
ACCESSION   DAOQUC010000659 DAOQUC010000000
VERSION     DAOQUC010000659.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60796_length_2232_cov_3.469913"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="PKH07_11160"
     CDS             <1..198
                     /locus_tag="PKH07_11160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35545.1"
                     /translation="APCGLDKSAVGGTAAILAVARNENLTPEQFYRWAQCPWVPALRA
                     LARNRSTPPELKAAIHRAIVV"
     gene            202..990
                     /locus_tag="PKH07_11165"
     CDS             202..990
                     /locus_tag="PKH07_11165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35546.1"
                     /translation="MRNLVIWGGPVSRSDLKVDVPLDTEVVVFNQGVGGIGSNAFRDL
                     ALSYLDADDHILPGLLRSKGIQEEDFDRIVLAGFSAFHGMANEILKHDADRVIGLISL
                     DACFSSKEALSKKGYEEFSRKAIRGEAVVVMTASLGGGQTFSTGEECVLATAEAAMSA
                     ENVDPVALQVEGIPEPEKAFKAGDLYVLDYAGKYSHQQHVWVLAQPILNAFLLPILEG
                     KLPSSNALQPMGTSKSNGAWGIFLGIAAALATVGSIKLLSSGKE"
     gene            complement(1024..1428)
                     /locus_tag="PKH07_11170"
     CDS             complement(1024..1428)
                     /locus_tag="PKH07_11170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35547.1"
                     /translation="MNEASMIGHATVRAGQPIGIKPLVVIQWLRESVLMLIVISQETL
                     SPVPNVSHVSKKLPPFFWDFNPYFTQVIEVAEVLLCQPTHLCIALGNSSVIKLESNDV
                     KIAASEKLVERVVNNDVIWWHQKLVVFILQNK"
     gene            1462..>2232
                     /locus_tag="PKH07_11175"
     CDS             1462..>2232
                     /locus_tag="PKH07_11175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35548.1"
                     /translation="MPQKKTKKSEAKKASPAKKGGTAKARKPPTQEQCQKTFERAIKR
                     GSKNAAKVVHEMDKDAKQILKEDEAKKPAAKKAAAKKPAAKKAAAKKPAAKKPAAKKP
                     AAKKAAAKKPAAKKPAAKKPAAKKPKAKVLPSTTRAVERTAEASGLESATIDRILSFI
                     EADRSKQGRRLHAKGKTVPMAKSKKPTLAEAHERLARHYGISQKEVLENLESNSARPN
                     YRNNPVKGKKGKKGKKGTGRKQNPHHAHGSQTHRRDNPI"
BASE COUNT          563 a          595 c          636 g          438 t
ORIGIN      
        1 gctccatgtg gtctggacaa atctgcggtg ggaggcactg ctgcgattct cgctgtcgcg
       61 cgaaacgaaa acctgacacc tgaacaattc tatcgttggg cacagtgccc gtgggtacct
      121 gctcttcgcg cactggctcg gaatcggagc actcctcctg agctgaaggc tgccatccac
      181 cgagccattg tggtgtgaat catgcgcaac ttggtgatct ggggtggccc tgtctctcgt
      241 tctgatttga aggtagacgt ccctcttgac accgaggtgg ttgtcttcaa tcaaggcgtt
      301 ggaggcatcg gaagcaatgc cttcagggat ctcgcgttga gttacttgga cgcagacgat
      361 catatcttgc ctggtctctt gcgaagcaag ggcatccaag aagaagactt tgatcggatc
      421 gtgttggcag gcttctccgc atttcacggt atggccaacg agatcttgaa gcacgatgca
      481 gatcgcgtca tcggactgat ctcattggat gcttgttttt cgtcgaaaga agcactgtcc
      541 aagaagggat acgaggaatt ctcgcgcaag gcgatacgcg gagaagccgt ggttgtcatg
      601 accgcttcgc tcggtggagg acagacgttc tctacaggag aagaatgtgt cttggccaca
      661 gcagaagctg ccatgagcgc agaaaatgtg gatcccgtcg cgctgcaagt ggaagggatt
      721 ccagagccag aaaaagcatt caaagcaggt gatctctacg tgcttgacta cgctggcaag
      781 tactctcacc agcaacatgt ttgggtgctt gcacaaccca tcttgaacgc gtttctgttg
      841 cccatactcg aaggaaaatt gccgtcgtcg aacgcgctgc aaccaatggg aacctccaag
      901 agcaacggag catgggggat attccttgga attgctgctg ctttggcaac tgttgggtcc
      961 atcaagctgc tgtcaagcgg aaaggaatga atcatgggat tgctcacgaa gggatccgtc
     1021 gtttcattta ttttggagga taaacaccac gagcttttgg tgccaccaaa tcacatcatt
     1081 gttcacgacc ctctcgacga gcttctcaga agctgcgatt tttacatcgt tcgattcgag
     1141 tttgatcaca ctggagtttc ccaaagcgat gcagagatgc gtcggttggc agagaagtac
     1201 ttcggcaacc tcgatgactt gtgtgaagta ggggttgaaa tcccagaaga agggtgggag
     1261 tttcttactg acgtgagata cattcggtac aggcgagagg gtttcttggc tgatgactat
     1321 cagcataagt acgctttccc tcaaccattg tattaccacc aagggtttga tgcctatcgg
     1381 ttggccagcc ctgacagttg cgtggccaat catcgaggct tcgttcaccc ctaaaagact
     1441 cgttcagagg agatcggcga aatgccccag aagaagacga agaagagtga ggccaagaag
     1501 gcttctccag cgaagaaagg tggcactgcg aaagcacgca agccgccgac gcaggagcaa
     1561 tgccaaaaga ccttcgagcg cgccatcaag cgcggcagca agaatgccgc caaggtggtt
     1621 cacgaaatgg acaaggacgc caagcaaatc ctcaaggagg atgaggcgaa gaagccagcc
     1681 gccaagaagg ccgctgccaa gaagcccgcc gccaagaagg ccgctgccaa gaagcccgcc
     1741 gccaagaagc ccgctgccaa gaagcccgcc gccaagaagg ccgctgccaa gaagcccgcc
     1801 gccaagaagc ccgctgccaa gaagcccgcc gccaagaagc ccaaggcgaa ggtactccct
     1861 tcgaccacac gcgctgttga gcgtacagca gaggcgtcgg gtctcgaatc ggcgacaatt
     1921 gatcgcatct tgtccttcat tgaggcagat cgatccaagc agggtcgtcg gcttcatgcc
     1981 aagggcaaga ccgtccccat ggcgaagtcg aagaagccaa ccctggccga ggctcacgag
     2041 cgtctcgcac gacactacgg catcagccag aaggaagtgc tggagaactt ggaatccaac
     2101 tcggctcgcc ccaactaccg caacaacccc gtcaagggga agaaggggaa gaagggcaag
     2161 aagggcaccg gtcggaagca gaatccgcat cacgcgcacg gcagccagac gcatcgtcgt
     2221 gacaacccga tt
//
LOCUS       DAOQUC010000660         2232 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_60797_length_2232_cov_3.418925, whole genome shotgun sequence.
ACCESSION   DAOQUC010000660 DAOQUC010000000
VERSION     DAOQUC010000660.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60797_length_2232_cov_3.418925"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(151..519)
                     /locus_tag="PKH07_11180"
     CDS             complement(151..519)
                     /locus_tag="PKH07_11180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35549.1"
                     /translation="MRRIPVGVGVELNVGVAEGVLVAAFVPVGVLVGVFDGVVVGVFV
                     HVGELVGVEVLEGVLEGLLVGVLVGVSVQVGVLVNVGVREGFLVGVQVGVSVTVQVGV
                     LLGVRVAVLVGVSVEVNDGV"
     gene            822..971
                     /locus_tag="PKH07_11185"
     CDS             822..971
                     /locus_tag="PKH07_11185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35550.1"
                     /translation="MIKSEHLRTFPVGMLCFVSIYGRQANLLIAGSVMRQICGSGRSE
                     MNPMQ"
     gene            complement(973..>2232)
                     /locus_tag="PKH07_11190"
     CDS             complement(973..>2232)
                     /locus_tag="PKH07_11190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35551.1"
                     /translation="ALYSLDKGKPHKARFPNHNEDRRCLRVNVIECPETDRAIRLLTE
                     GLSFNSMFYPTCRTWTADGQNILFETTAFGPDPNGILGERQIQEVHSESGATQWLATV
                     PFTLRPRSEASDLKTSSLYHCDYAPLADRIYFQDVNGHRLFSLNRATGDVKEIWQELD
                     GTIGDPPSCTADGSRIAFYVIFPGPHKNRYFSGITSAILLLDVNLETGERIGEPRLVT
                     AFVGRTATPRNIYVNHCQINSAHPDQIIFAHEFGGCPYDGSLLRERIWFVNSDGTNEH
                     PLIRGAHGRYHTHEVIGASGEWVYYVDTGGLCRVHIGTQKVEEIVPPGHPERLWHISV
                     SPDEKWVVADTEIFREKTPEGLWMSSLMLVSVETGQMQCVCRFPCRLKHPAHPHPNFS
                     PDGSKIAFVVGTPTGSSVACCDVSDLL"
BASE COUNT          531 a          633 c          596 g          472 t
ORIGIN      
        1 cgggtcgcgg gtcaggatcg atggcagcgg gaatttcagt ggatatgcct ggggagaaaa
       61 cgtcgggtgg attaacatga gcagcggata cggagtgaaa tgggacaaac caactcctac
      121 tccgacagac acgcctacct atacgcctac tcacaccccg tcgtttacct caacagatac
      181 tccaaccagc actgcgacgc gcactccaag cagcacgcca acctggacag taacggacac
      241 ccccacttgg acaccgacaa ggaagccttc gcgcacaccg acattcacca atacgccaac
      301 ctggactgat acccctacca gcacacctac cagaagtcct tccagaaccc cttcaaggac
      361 ttcaacaccc accagctctc cgacgtggac gaacacaccc accacgacac cgtcaaacac
      421 ccctacgaga acaccgacag ggacgaaggc ggcaacgaga accccttctg cgacgccgac
      481 attcagttcg actcccactc cgacggggat tctgcgcatc ccagattaca accacagtca
      541 cacagttgac gagatcgacc tgcttctgct gctgggtcat tatggacaga gtgagggtga
      601 gtatgacctg gatggagacg gcgaactcag ttctgaagac ctgtttctct tttctctctg
      661 gtggaagaag aacgtgtgac gaagcaggcg gcaccaaaca acgtgctcgt aaacggggcg
      721 cattctagaa cagcatcatg cctttgtcga gaccaggggt cattgcgctg gcgattctag
      781 aattcatggg gaaacacggc ctcttcctcc ggactctcat catgatcaag agtgaacacc
      841 tgcgaacgtt tcccgtcgga atgctgtgct tcgtcagcat ctacggcaga caagccaacc
      901 ttctcatcgc aggcagcgta atgcgtcaga tctgtggttc aggcagaagt gagatgaatc
      961 ccatgcaatg actcagagca gatccgacac atcacagcag gcaacgctcg agccggttgg
     1021 agttccgaca acaaaggcga tcttgctccc atcagggctg aagttgggat gaggatgtgc
     1081 cgggtgtttg aggcggcagg ggaagcgaca cacgcactgc atctgtcctg tttcgacact
     1141 caccagcatc aaactgctca tccagagacc ttcgggcgtc ttctcgcgga atatctctgt
     1201 gtccgcgacg acccatttct catcaggcga gacgctgatg tgccagaggc gttccggatg
     1261 tcctgggggg acgatttcct cgaccttttg tgttccaata tgaactcgac agagtcctcc
     1321 tgtgtcaaca tagtagaccc actcgcctga agcaccaatt acctcgtggg tgtgatagcg
     1381 accatgcgcg cctcgaataa ggggatgttc attggtgccg tctgaattga cgaaccagat
     1441 gcgctcgcgc agcagcgagc catcataggg gcatccaccg aattcatgcg caaagatgat
     1501 ctggtccgga tgggccgagt tgatctgaca gtggttgacg tagatgtttc gaggcgtggc
     1561 agttcgtccc acaaatgctg tgaccagccg tggttcacca attcgctctc ccgtttccag
     1621 gttgacatcc aaaagcagaa ttgccgatgt gatcccgctg aaatatcggt tcttgtgggg
     1681 acctggaaag atgacgtaga acgcgatccg actgccatcc gcagtgcaac tgggcggatc
     1741 tccgatggtg ccatcaagtt cctgccatat ttccttcaca tcccccgtcg ctctgttcag
     1801 actgaagagc cgatgcccat tgacgtcttg aaagtagatg cggtctgcca ggggcgcgta
     1861 gtcacaatgg tagagagagg atgtcttgag atcggaggct tcagaccgtg gacgcaaagt
     1921 gaatggaacg gtcgccagcc attgcgttgc gcccgattca gaatgaacct cctggatctg
     1981 acgctctccg agaatgccat tgggatcggg cccaaacgct gtggtttcga acaagatatt
     2041 ctggccgtcg gccgtccatg tccggcaggt ggggtagaac atgctgttga aggacagtcc
     2101 ttcagtcaga agtcgaatcg cacgatctgt ttcgggacac tctatcacgt tcactctgag
     2161 gcaccttctg tcttcattgt ggtttgggaa tcgtgccttg tgaggcttcc ctttgtcaag
     2221 cgagtagagc gc
//
LOCUS       DAOQUC010000661         2230 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_60890_length_2230_cov_2.044598, whole genome shotgun sequence.
ACCESSION   DAOQUC010000661 DAOQUC010000000
VERSION     DAOQUC010000661.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2230
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60890_length_2230_cov_2.044598"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..276)
                     /locus_tag="PKH07_11195"
     CDS             complement(<1..276)
                     /locus_tag="PKH07_11195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015226655.1"
                     /note="catalyzes the formation of 3-phosphonooxypyruvate
                     from 3-phospho-D-glycerate in serine biosynthesis; can
                     also reduce alpha ketoglutarate to form
                     2-hydroxyglutarate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HNT35552.1"
                     /translation="MFKVLVADKMAEEGLKVLRDHALFEVDVKLKLSPEELVACIADY
                     DVLLVRSDTKATAAVIEAGKKLKVIGRAGVGLDNVDLDAATRRGIIVM"
     assembly_gap    428..437
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(438..1595)
                     /locus_tag="PKH07_11200"
     CDS             complement(438..1595)
                     /locus_tag="PKH07_11200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013706246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HNT35553.1"
                     /translation="MLKKYIMSPGPTEVPPSVLLRTAEPIIHHRTSEFEKVLASVSEG
                     LKDIFKTSNSVLTFASSGTGAMQGAVSSFLSPGDKAICIRGGKFGERWGEICKAFGVE
                     VIAIDVPWGESVDPTSVDKALKANPDVKAVYSTHCETSTGTLHDIQALGAIVSKTPAI
                     LVVDAVSSMGSEKCLTDEWGIDIVVTGSQKALMLPPGLAFASVSEKAWALTKTAKCPE
                     YYFSYTKARKSLEKNTTAYTPAVSLIMGLDEALKIIKQEGIEAVWTRTEEMGKAMRAG
                     MIRMGLELYSKRPASALTSVNVPQGVNGDALPKKVRVEYGLTIAGGQDELKGKIFRVS
                     TMGYADLMDVPMALTVIGMALKELGFDAEIGRAIDAAVEVLYAYKVRKTAK"
     gene            2014..>2230
                     /locus_tag="PKH07_11205"
     CDS             2014..>2230
                     /locus_tag="PKH07_11205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35554.1"
                     /translation="MQLQTGEKHGQDALRLSILSLILVGTAYFAVGLLTLSPAYVDFG
                     DGNYLYLSKRISEGVRVYRDMVSPQPPL"
BASE COUNT          519 a          629 c          555 g          517 t
ORIGIN      
        1 catgacaatg atgccccgac gtgttgccgc atccagatca acattgtcca aaccaacgcc
       61 ggcgcgtccg atgactttca acttcttacc agcctcaatc actgctgcgg tggccttggt
      121 atcactgcga accagaagaa catcataatc agcgatacag gcaaccagtt cctctgggga
      181 cagtttcagt ttgacgtcca cttcgaacag ggcgtgatct cgaagaactt tcagaccctc
      241 ttcagccatc ttgtcggcaa ccagaacttt gaacatcgtt ctctcctctc tctgaatgca
      301 ttcagcgggc ttatcagacc cgaaaactcg aaaaagtatg ctcaagcaca tcacgaagtg
      361 atggtgcacc acacaaatat caaagcagga aacgagcttg gcatagatgg cattgtttgc
      421 caagatcnnn nnnnnnntta cttggccgtc ttcctgacct tgtacgcata gagaacctcg
      481 acggcagcgt ctatcgctct gccgatctcg gcgtcgaatc caagttcctt caaagccatg
      541 ccgataacgg tcagagccat cggaacgtcc atcaaatcag cataccccat ggtcgagacg
      601 cggaaaatct tccctttcag ctcgtcctgt ccacccgcaa tggtcaaacc gtattcgaca
      661 cgcactttct tgggcagtgc atctccattg acgccttgag gcacgttgac cgatgtcagg
      721 gcgcttgccg gtctcttgga gtagagttcc agacccatgc gaatcatacc ggcacgcatc
      781 gccttgccca tctcttctgt tcgcgtccag acggcttcga tgccttcctg tttgatgatc
      841 ttgagggctt catccagacc catgatcagg gagactgccg gagtgtaagc tgtggtgttc
      901 ttctccagag atttcctggc cttggtatag ctgaaatagt actcgggaca ctttgccgtc
      961 ttggtcaatg cccaagcctt ctcgctgacg ctggcaaagg caagacccgg gggaagcatg
     1021 agcgccttct gcgagcctgt cacaacgata tcaatgcccc attcgtcggt gaggcatttc
     1081 tcagaaccca tcgaactgac cgcatccaca accaaaattg ccggtgtctt ggacacaatg
     1141 gcacccagtg cctggatgtc atggagtgtg cccgtgctgg tctcgcagtg ggtggaatag
     1201 accgccttca cgtccggatt tgccttcagt gccttgtcca ccgaagtcgg atccacagac
     1261 tcaccccacg gaacatcaat ggcgatgact tcgacgccaa atgccttgca gatctctccc
     1321 caacgctctc caaacttgcc tccacgaatg cagatcgcct tgtcgccggg actgagaaaa
     1381 ctcgaaacgg caccctgcat ggctccggtt ccggatgatg caaaggtaag aacactgttg
     1441 gaagtcttga agatatcttt tagaccctca ctgactgagg cgagaacctt ctcgaattcc
     1501 gaagtgcgat gatggatgat tggctccgcg gttcgcagca gtaccgaagg cggaacctcc
     1561 gttggccctg gggacatgat gtacttcttt aacacgatat tctcctcctc ctgaaaagct
     1621 cgacttcagt gactctgtcg ttcgccggaa atgagcaccg ctgcgttgtg gacacaaccc
     1681 acatggccgc tgactccatc cggctcggca gggaaacccc gcttatggct ttgaggcggt
     1741 aatataccga gttttcaact cagttgtcaa acgaatgttc cgagggaata ggcatcgttg
     1801 caaggcttgg atcggcaatc cgatgccgtt ggaatcaagg cacagcatgc cgttcctttt
     1861 tgactgcaaa acgcctggca agtcgctata atgcgcccca gttgaagacc gcccggatgc
     1921 gggtgcaggg catttgatcc gcctgaaggc gttcctgtct cgctctgcgg aaagaggcaa
     1981 aggacggaac gggtcatgac agaaggagag agtatgcagt tacagacagg tgaaaagcat
     2041 ggacaggacg cattgaggct ttctatcctg agtttgatcc tcgtgggaac ggcatacttt
     2101 gccgttggac tcctgacact cagccctgcc tatgtggact ttggcgatgg aaactatctc
     2161 tatctctcca agaggatttc agaaggggta cgggtgtacc gagatatggt atcaccacag
     2221 ccaccacttc
//
LOCUS       DAOQUC010000662         2228 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_60948_length_2228_cov_3.592729, whole genome shotgun sequence.
ACCESSION   DAOQUC010000662 DAOQUC010000000
VERSION     DAOQUC010000662.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2228
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60948_length_2228_cov_3.592729"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1785
                     /locus_tag="PKH07_11210"
     CDS             <1..1785
                     /locus_tag="PKH07_11210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35555.1"
                     /translation="DGEYSMLPEEKLLFASAFGMGILSVLMFLLASVHLYNKVSAICL
                     MGCVAILGRKTWRPLVNSLKERCLGLRLSARSLPLILSVAIALCFALLPTVNYDALEY
                     HLGAQSEYIRNGGFCLLPFNVYSNFPMGVEMLYGLGLLFGGDGVPQVLHCLFALLTAA
                     TVSCAVRRLSKTAADVFSPLAFLATPLVMLSCGQANIDLALAFYSSLVVLALILFPFP
                     LNRQQSLFIGLLCGLSLAAKYTAVVQICFPAALALTIRTVKTRSPNNKSLMIKNALFF
                     GSGVLVPFLPWAAKNLVLQGNPFFPLAYEWFGAKFWSHEAYALFQNAHASRLHGGILQ
                     WFSALGTNLLDLVAGQKNLSVFAVALPLALLRKPSRTSSALWVYVLSSFAGWFLLTHW
                     QDRFLLPLVPACVVLAMEAFHTTASLTLKSLLRICMTSLILINLWYAFAVLSQGNAFD
                     YLTEKTDLHEWRARGLPHFQAIDFMNESLETGRHHVLFVGEARTYGNAVRVVASTVFD
                     RSPFLETTQEYVTAHDKVQALRNAEFTHLLYNGYEIKRLHDTYAPYGWRMAPAIESTM
                     REIAPLLKEVFHAGHPGLEGVSVYELSR"
     gene            complement(1797..>2228)
                     /locus_tag="PKH07_11215"
     CDS             complement(1797..>2228)
                     /locus_tag="PKH07_11215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35556.1"
                     /translation="KFVLVLLLIFAALYVFAVLKEGGNAAKEPTYATDSPRSTLTTYL
                     NAVKRNDRQGIALAFENEGYGMNALADINSYETPGVDRIEFTMPIQSGGNSASGSAVV
                     VGTDDKIVTTVHFEMKNIKDSWKITGISTVGGFRKPTTSMH"
BASE COUNT          469 a          625 c          552 g          582 t
ORIGIN      
        1 gatggagaat acagcatgct gcccgaggaa aaactgctgt tcgcctcagc attcgggatg
       61 ggcatcctct ccgtgctgat gtttcttctc gccagtgttc atctctacaa caaagtatct
      121 gccatctgcc tgatgggatg cgtcgcgatc ctggggagaa agacctggcg tcctctggtc
      181 aacagcctga aagagcgctg tcttggtctt cgcttgagcg ctcgctcatt gccgctcatt
      241 ctctccgtcg ccatagcgct ctgctttgcc ctgcttccaa ccgtcaacta cgatgcattg
      301 gaataccatc tgggtgccca gagtgagtac atccgaaacg gtggtttctg tcttctcccc
      361 ttcaatgtct actccaactt ccccatgggc gttgagatgc tctatgggct tggcctgctt
      421 ttcggcggcg acggagtacc tcaggttctt cattgcctgt ttgcattgct gacagcagcg
      481 acagtttcat gtgctgtgag gaggctctcc aaaactgcgg cagacgtatt cagccctttg
      541 gccttcttgg caacgcctct ggtgatgctg tcctgtggac aggcaaacat tgatcttgca
      601 cttgccttct actcctcatt ggtggttctt gcgctgattc tgttcccttt ccccttgaac
      661 cggcagcaga gccttttcat aggtcttctg tgcggtctgt cactcgcagc caaatacact
      721 gccgtcgtac aaatctgctt cccggctgcc ttggccttga ccatcagaac tgtgaagaca
      781 cgttccccca acaacaagtc attgatgatc aagaatgcgc ttttttttgg ctcgggagtg
      841 cttgtgcctt ttctgccatg ggcagccaag aatctcgtcc ttcaaggaaa cccattcttc
      901 ccgcttgcgt atgaatggtt cggagcaaag ttttggtcac acgaagccta tgcgctgttc
      961 caaaacgcac atgcgtcaag gcttcatggg ggaatcctcc agtggttctc tgctttggga
     1021 acaaacctgc tagaccttgt tgctggacag aaaaacttga gtgtctttgc cgtcgcactc
     1081 cccttggctc tgctgagaaa gccaagccgc acatcatctg ctctgtgggt atacgtcttg
     1141 agcagctttg ccggatggtt cttgctgact cactggcagg acagatttct tctgccactg
     1201 gtgcccgcat gtgtcgtcct ggcgatggag gcctttcaca ccaccgcttc gctcacgctc
     1261 aagagcctgc tgagaatatg tatgaccagt ttgatcctca tcaatctctg gtacgcgttt
     1321 gcagttctca gtcaaggaaa tgccttcgac tatctgactg aaaagacgga tctccacgaa
     1381 tggagagcac gcggactgcc ccatttccag gccattgatt tcatgaatga atctcttgaa
     1441 accggtcgtc atcatgtgct gttcgtcggt gaagccagaa cctacggcaa cgcagttcgc
     1501 gtcgtcgcca gcacggtctt cgaccgaagc cccttcttgg agacaaccca ggaatatgtc
     1561 acggctcacg acaaagtcca ggcacttcgg aacgcagagt tcacgcatct tctctacaac
     1621 ggctacgaga tcaaacgctt gcacgacacc tatgcgccat acggctggag gatggctcct
     1681 gccatcgaat ccacaatgcg cgagatagct cccctgctga aggaagtctt ccatgccggt
     1741 catcccggcc tggaaggagt ttccgtatat gaactatcca gataggctgc ccactctcag
     1801 tgcatagagg ttgtgggttt cctgaatcca ccaacggttg agattcccgt gatcttccag
     1861 gaatctttaa tgttcttcat ttcgaagtga acggtggtca ctatcttgtc atcggtgccc
     1921 acaacgactg cgctgccgct ggcggaattg ccacccgact gaattggcat ggtaaactcg
     1981 attcgatcca caccaggagt ctcatacgag ttgatgtctg ccagagcgtt catgccataa
     2041 ccctcgttct caaatgccag agcaattcct tgacggtcat tgcgtttgac ggcattgaga
     2101 taagtggtca gagtcgagcg tggggaatcc gtcgcgtacg ttggttcctt tgcggcgttg
     2161 ccaccttcct tgagaacagc gaaaacatag agagccgcga aaatcagcaa gagaacgaga
     2221 acgaactt
//
LOCUS       DAOQUC010000663         2228 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_60958_length_2228_cov_3.309710, whole genome shotgun sequence.
ACCESSION   DAOQUC010000663 DAOQUC010000000
VERSION     DAOQUC010000663.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2228
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60958_length_2228_cov_3.309710"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(98..1831)
                     /gene="lnt"
                     /locus_tag="PKH07_11220"
     CDS             complement(98..1831)
                     /gene="lnt"
                     /locus_tag="PKH07_11220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HNT35557.1"
                     /translation="MNKSQYEFISQDRSGSPRQLWRTLAPAFLFFAIISVLLIADPSW
                     RLEVFLIPVFVFTAFVTRRYGWDRGSLLAILSGFWMNLSMPDIQFSAIIFVALIPLFA
                     VSEKADRLWKPVVYGCIACLSCAHFMNGWALNALSRMCEMSRLAIYPLFIGFFFAHLW
                     KFLWFALAAYFNARHLRLPRIIILPTAFAVLELFKLEMYPWPLGLALIEHTRWIQIVD
                     LVGLPGLSFLIVFVNVALFEIGVRVRQKNYRNAVRFAVACLVAIALWGLYGQLRMAQV
                     QQVQIQGTPIQVAVVQPNSPFKVTSDDVDAERHVNESLYSLAHRAVKDVSADLLVFAE
                     AAGSVGYQTNDNPDLCEMYRRIAREIACPIVFDNVHVKRNDHGWTLHRTALLLSPQAE
                     ILGEYIKRRLVPFGEYVPFVRQLPFLKRVFPNAAGHLNYQPGTEAPVWSVAGVSIVPQ
                     ICYEIIYPQLTRSHKTGGSGLIVNISNDAWYGGDKQPQQHFLASVFRCVENRMPMVRV
                     ANTGISAYVAASGEVMEPVSPMNEPWVDCRTVMVPQLGSVYRRFGDWFGWLCLVVLLG
                     SGLWRRLQSRS"
     gene            complement(1948..>2228)
                     /locus_tag="PKH07_11225"
     CDS             complement(1948..>2228)
                     /locus_tag="PKH07_11225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35558.1"
                     /translation="VGSAETTGWFNDWQEPYHLDGRFIVESVWLTEGQAFTVDITGSN
                     EDPNCLVRFDEITLDLLDLTDPPTATPTNTPAPTPTKSTGVSTFEVYE"
BASE COUNT          589 a          599 c          583 g          457 t
ORIGIN      
        1 cgacgacctg cggtttacga aaccgctgct ctgccagctg agctacatcg gcactctgaa
       61 gtccaagcgg gagaataccc atgtccactt ggttttgtca agaccgagat tgcagacgtc
      121 gccagagtcc agatccgagc agcacaacca gacacagcca tccgaaccag tctccaaaac
      181 gccgatagac tgagcccaac tgaggcacca taacggttcg acagtccacc catggttcgt
      241 tcatcggcga aaccggctcc atcacctcac cggaggcggc aacgtaagcg gaaattcccg
      301 tattggcaac acgcaccatc ggcattctgt tttcgacgca tcggaagacc gacgccagga
      361 aatgctgctg cggttgcttg tctccaccat accaggcgtc attggagatg ttgacgatca
      421 ggccgctccc ccctgtctta tggcttcgag tcagttgcgg atagatgatc tcgtagcaga
      481 tctggggaac aatgctcaca cccgcaacag accataccgg cgcttcagtc cctggctgat
      541 agttgagatg accggcagcg ttgggaaaaa cccgcttaag gaaaggaagc tgccggacaa
      601 acggaacgta ctctccaaag ggcacgagtc ttcgtttgat gtactctcca aggatctccg
      661 cttgtggtga cagcaggagt gcggtgcggt gcagcgtcca accgtgatca ttccttttta
      721 cgtggacatt gtcgaagacg atggggcaag cgatttcccg tgcgatgcgg cgatacattt
      781 cacacaaatc agggttatca ttggtctgat agccgacgga tcctgccgcc tctgcaaaga
      841 ccagcaggtc agcgctgacg tccttgactg cacgatgcgc caatgagtaa agcgattcgt
      901 tgacatgtcg ttctgcatcc acgtcgtctg aggtgacctt gaatggagag tttggctgaa
      961 cgacagcgac ctggatcgga gtcccttgta tctgcacttg ctgaacctga gccatgcgca
     1021 gctgaccata gagtccccac aaggcaatcg ctaccagaca ggcaaccgca aagcgaactg
     1081 cattcctgta gtttttctga cgcacccgaa ccccaatctc gaatagagcg acgttgacaa
     1141 agacaatgag gaaggaaagc ccgggaagtc caaccaagtc aacaatctgg atccatcgtg
     1201 tatgctcaat caaggcaagc cccaacggcc aggggtacat ctccagtttg aacaattcca
     1261 acactgcaaa tgcagtcggt agaatgatga ttcttggcag tcggagatgt ctggcgttga
     1321 aatatgccgc cagtgcaaac cagagaaatt tccacagatg ggcgaagaag aaaccgatga
     1381 acagcggata gatggccagg cgggacatct cgcacatgcg tgagagtgca ttgagcgccc
     1441 agccgttcat aaagtgagca catgacagac atgcgataca cccatacacc acgggtttcc
     1501 agagacgatc cgctttctcg gaaactgcaa aaagtggaat cagtgcaaca aagataatcg
     1561 cagagaactg gatgtcgggc atggacaggt tcatccagaa acccgagaga atcgccagca
     1621 gagagcctct gtcccatcca tatcttctgg tgacaaaggc cgtgaacaca aagacgggaa
     1681 tgagaaagac ctctagtcgc cacgacggat cggcaatcag tagaactgag ataatggcaa
     1741 agaataagaa agctggggcg agcgttctcc agagttgtct tggagaacct gatctgtctt
     1801 gtgagataaa ctcgtactgt gacttattca tgtgccgttg tggctgcaat ttggccggat
     1861 cgcctctcca acgtccttga aacagaaaca tgggatcgaa cacgaagtcc gatcccatgt
     1921 cttcttatca taggcacaga aaggattcta ctcgtatacc tcgaatgtcg aaacgcccgt
     1981 agatttcgtc ggcgtcggag ctggggtgtt ggtcggagtc gctgtcggcg gatcggtaag
     2041 atccagcaag tcaagagtga tctcatcaaa ccgaaccagg cagttgggat cctcgttcga
     2101 accggtgata tcaaccgtga aagcctgtcc ctcggtaagc cagacgctct caacaatgaa
     2161 gcgaccgtcc aagtggtacg gttcttgcca gtcattgaac caacccgtcg tctccgctga
     2221 acccacat
//
LOCUS       DAOQUC010000664         2228 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_60966_length_2228_cov_2.774965, whole genome shotgun sequence.
ACCESSION   DAOQUC010000664 DAOQUC010000000
VERSION     DAOQUC010000664.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2228
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60966_length_2228_cov_2.774965"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            91..825
                     /locus_tag="PKH07_11230"
     CDS             91..825
                     /locus_tag="PKH07_11230"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HNT35559.1"
                     /translation="MLAEKVESHEDVQEGMNVGYSLFQGYFFCKPEVVKGKTVPEYKI
                     NYLRFLQELSRPDLDFDELEGIIKQEVSLSMKLLRLLNSAAMGLRREVNSIKHALVLL
                     GERPLKKWASLIAFTNLGDDKPSELLITCLVRARFCEFLARSARMADRELDLFLTGMF
                     SAIDAVLDRPLEGLIEEMSLSDEVAKTLFGEDTTLSPVFNLALAFERADWDSISHLVA
                     QLKVSENTVSEAYKEAVVWSREVFSI"
     gene            complement(860..>2228)
                     /locus_tag="PKH07_11235"
     CDS             complement(860..>2228)
                     /locus_tag="PKH07_11235"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HNT35560.1"
                     /translation="NVNAAWGTDYEDFVQIQYPVTDPDAAPLAEDRREFQRKEFPLRF
                     VRVPEEYAQAFRNYLQEILGTLKAVNLELLTHFDSWEQIPFETEYAGSRPYRKYWSQF
                     VIERIPIEQREFSGFEIDYAKYLQDKYDDSESLSKAYAMSIPSIEGFRIPFDYLDYES
                     FHQDRSGWRWFFMGYNYSVVLQFLSTRGRAVWNTFILVALTVMAGLVVNPITAYALSR
                     FNLRGTQKILVFLLVPMSFPPEVSMIPSFLLLRSFPLGLLILGGGAGALAFVALRRLF
                     PSWNKGYRGMLQMVLAFLIGRLCALPLCHVFHVPPSITLLNTYAALVLPGMASGFSIF
                     LLKGFFDGLPRELYEAASMDGASEMQMFRVITYPLCKPIIVVTLMGQVLAAYGGFMWA
                     FVVCQDKNMWTLMVWLYAFQSEYAVGQLNLIMAALTLASIPTFIFFIFCQKIIMRGII
                     LPSMK"
BASE COUNT          591 a          547 c          576 g          514 t
ORIGIN      
        1 cacctattat tactcccgat aaattgataa aaatcgaaat ccaaggagcg caagttgtac
       61 gcccagcatt tcatccctcg tggattgcag atgcttgcgg aaaaggttga aagccacgaa
      121 gacgttcagg aaggaatgaa tgtcggttat tcactttttc agggctattt tttctgcaag
      181 ccagaggtgg tcaagggcaa gactgttccc gagtacaaaa tcaactatct gcggttcttg
      241 caggaattga gccgacccga tctggatttt gatgaacttg aagggataat caagcaggaa
      301 gtctcgcttt ccatgaagct cttgagactg ctgaactcag ctgcaatggg tcttcgtcgt
      361 gaagtcaatt cgatcaagca tgcccttgta ctactcgggg agcgtcctct gaagaaatgg
      421 gcaagcctca tcgcgttcac aaatctggga gatgacaagc ccagtgaact gctcattacg
      481 tgccttgttc gggcaagatt ctgcgagttt cttgcgcgca gtgcaaggat ggctgaccgg
      541 gaactggatc tcttcctgac gggaatgttt tcggccatcg acgctgttct cgaccgacca
      601 cttgaagggc ttattgagga gatgtctctt tccgatgaag ttgccaagac actttttgga
      661 gaagacacga ctctgtcacc cgttttcaac cttgcgttgg cctttgagcg tgcagactgg
      721 gactccatct cccatcttgt ggcgcagctg aaggtttctg agaacaccgt ctcagaagca
      781 tacaaagagg ctgtggtctg gtcgcgagaa gtgttctcca tctgatggag agccggtgcc
      841 taaccatccg aatgtgtgct cacttcatgg acggcaggat gattccccgc atgatgattt
      901 tctggcagaa gatgaagaat atgaaggtgg ggatggatgc cagagtcaga gcggccatga
      961 tgagattgag ttgcccaact gcatattccg attggaatgc gtagagccac accatcagcg
     1021 tccacatgtt cttgtcttga caaacaacga aggcccacat gaatccgcca taggccgcaa
     1081 gcacctgtcc catcaatgtg acaacaatga ttggtttgca gaggggatac gtgatcaccc
     1141 tgaacatctg catctccgag gcaccgtcca tggacgcggc ctcgtagagt tccctgggca
     1201 aaccgtcgaa gaaccccttc aaaaggaaaa ttgagaatcc cgaggccatt ccaggaagca
     1261 ccaacgcagc ataggtattc agcagagtaa tgcttggtgg cacatgaaaa acatggcaga
     1321 gaggcaatgc acacagacgt ccgatcagaa atgcgagcac catctgaagc attcctctgt
     1381 agcccttgtt ccaggaagga aagagtcttc tcaacgcaac gaaagccagg gctcctgccc
     1441 ccccgccgag aatcagtaat cctagaggga aacttctcag aagaaggaaa cttgggatca
     1501 ttgacacttc cgggggaaat gacatgggca cgagcagaaa cacgagaatc ttctgtgtac
     1561 cacgaaggtt aaagcggctc agtgcgtatg cagttattgg attgacgact aagcctgcca
     1621 tgacagtcaa ggcaacaagg atgaaggtat tccagacggc gcgaccacgg gtcgagagaa
     1681 actggagtac caccgagtaa ttgtatccca taaagaacca acgccaacca gaccgatctt
     1741 ggtggaaact ctcgtaatcg aggtagtcaa agggaatccg aaaaccttcg atactcggta
     1801 tgctcatcgc gtacgccttg gacagggatt cggaatcatc gtatttgtct tgcagatact
     1861 tggcatagtc aatctcaaaa ccggaaaact ctctctgctc gattgggatc cgctcgataa
     1921 caaactggct ccagtacttg cgataggggc gactgccggc atattccgtc tcaaagggga
     1981 tttgttccca cgagtcaaag tgggtcagca gttccagatt gaccgctttc agagtcccca
     2041 atatctcctg gagatagttc cggaaagcct gagcatattc ttcgggcact ctgacgaatc
     2101 gaagtggaaa ctccttgcgc tggaattcgc gtctgtcttc agccaatgga gcagcgtcag
     2161 gatctgtgac ggggtactga atctgaacaa agtcttcata gtccgttccc caagcagcat
     2221 tcacgttg
//
LOCUS       DAOQUC010000665         2228 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_60969_length_2228_cov_2.738150, whole genome shotgun sequence.
ACCESSION   DAOQUC010000665 DAOQUC010000000
VERSION     DAOQUC010000665.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2228
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60969_length_2228_cov_2.738150"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2157)
                     /locus_tag="PKH07_11240"
     CDS             complement(<1..2157)
                     /locus_tag="PKH07_11240"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HNT35561.1"
                     /translation="MKGSAIVKPEGKELVTTNILSGGIPTQLFDLTQRDTRIENADQS
                     PKKPVVGINVWDGRDDLDGSSAIGRDEFVRNVSVVFLPNGEGFTPSDLSALTRDESSG
                     VALYKDVYNPSNPLSINGEYDRFDQLVPLTRVPSISNYSYDGFPGAFKVDLIPSLTGM
                     EIPDTDEDADLGSDYFVVIRTSRNISRGDAFSVYMAPDISLTYTTGKSSKSIRTGILN
                     PIINEVPTIQILEPAEPVSPDENFNAKITWADSDPDNDARIALYYSDSDNFVGTLIVD
                     DISEDDETDTYIWNVRNVPTGRYKVFGVIRDSDIPRDPILYPETRSYSNGSVVIRNDP
                     PTFAFVSPNAQTSGQYSDVFEILWSANDKENNASISLYYNSTPTASGGVLIADGLSED
                     TMSRYLWRLNRVPAGTYYIYAIVNDGVNTPPLAVVSEGTVNVVHVTREKSGFPLATGN
                     LIYSSPTLADVTGDGSAELFVGCDDGKLYGLDGNGQALPGFPVVIGEKVQSSPAVGDV
                     IGDGGLEIVVVAFDGKIYVVDPQGRFYTRQGGLSTARVAFESVQPNVSASAALHDFNG
                     DGKLDVVVPDGAGNIVIVLSSNGSKQTIPVGGAALYASPAIADLNGDSESEIILLVPK
                     TTTQPDRLVVVSPEGIPLAGFPLDVPSAVSGSPAVCDLSDSPGLEVVFGTLGRQLYAV
                     SSTGSVLAGFPLTMTSGLQASPIVTTFSNPDERFIVA"
BASE COUNT          544 a          613 c          577 g          494 t
ORIGIN      
        1 tgccacgatg aatcgttcgt ccggattcga gaaggtggtc actatgggtg aggcctgcaa
       61 ccctgaagtc atcgtcagcg ggaatcctgc aagcacagag ccagtcgagg aaacggcata
      121 caactggcga cccaaggttc cgaacactac ttcaagacct ggggaatcgc tgaggtcgca
      181 gaccgcagga gaaccggaca ccgccgaagg aacatccaac gggaatcctg ccaacggaat
      241 cccttccgga ctcacgacga ccagtcggtc gggttgtgtc gtcgtcttcg gaaccagcag
      301 gataatctct gactcagaat cgccgttcaa gtcagcaatc gcgggggaag cgtagagcgc
      361 agcaccccca acggggatcg tctgcttgga gccattggat gagagcacga tcacgatgtt
      421 cccagcccca tccggcacca caacatccaa cttgccatcc ccgttgaaat catgcagcgc
      481 cgcggaagcc gagacattcg gctgcactga ctcaaaggca actcgggcgg tggaaagacc
      541 gccttgtcga gtatagaagc gtccctgagg atcaacgacg tagatcttgc catcgaaggc
      601 aacaacgacg atctccagtc caccatcacc aatgacatca cctaccgcag gagatgattg
      661 aactttctca ccgatgacaa cagggaatcc tggaagcgcc tgaccatttc catcgagtcc
      721 gtagagttta ccatcatcac aacccacaaa gagctcggca ctcccatctc ctgtcacatc
      781 cgccagtgtc ggagatgagt aaatgagatt gcctgttgcc aaggggaacc cggatttctc
      841 gcgcgtcaca tggacgacgt tgaccgttcc ctcgctgaca accgccagtg gcggagtgtt
      901 cactccatca ttcacaatcg cgtagatgta ataggttccc gcaggcacac gattcagccg
      961 ccacagatag cggctcatgg tgtcttcgga aaggccgtcc gcaatgagga ctccccctga
     1021 tgcagtcggc gttgagttgt agtagaggct gatggatgcg ttgttctctt tgtcgttcgc
     1081 cgaccagaga atctcaaaga catcggaata ctgaccgcta gtctgtgcgt tgggtgaaac
     1141 gaaggcaaag gtcggcggat catttctgat caccacagaa ccgttgctgt aactgcgtgt
     1201 ctctggatac agaatcgggt cacgcgggat gtctgaatct cggatcacac caaatacctt
     1261 gtagcgtcct gtcggaacat ttctcacgtt ccagatgtag gtatccgtct catcgtcttc
     1321 gctgatgtca tccacgatca atgttccaac aaagttgtcg ctgtcggaat agtaaagagc
     1381 aatacgggca tcgttgtccg ggtcggaatc cgcccaggtg atctttgcgt tgaaattctc
     1441 atcggggctg actggttcgg caggctccag aatctgaatc gtcggaactt cattgatgat
     1501 cggattcaga atgccggtgc gaatggattt gctgctcttg cccgtcgtat atgtcagaga
     1561 aatatcggga gccatataga cactgaaggc gtcgccacgg gagatattgc gacttgttcg
     1621 gatgaccacg aaataatccg aaccgagatc agcatcttcg tccgtgtcgg gaatttccat
     1681 gccggtcaga ctgggaatca gatccacctt gaacgcacct ggaaaaccat catagctata
     1741 gttgctgatg ctgggaacac gcgtcagcgg aaccagctga tcaaaacggt cgtactctcc
     1801 attgatcgaa aggggattgc tcggattgta gacatccttg tacagagcaa cgcctgaact
     1861 ttcgtcccgt gtcagagcag acaaatcaga gggcgtaaac ccttcaccgt tcggcaggaa
     1921 aacaaccgag acatttcgga caaactcatc ccgaccgatg gccgagctac cgtccaagtc
     1981 atctcttcca tcccagacat tgattcccac cacaggcttc ttggggcttt gatctgcatt
     2041 ctcgattcgg gtgtcccttt gggtcagatc aaacagctgc gtcggaatcc caccactgag
     2101 tatgttggtg gtgactagtt ctttcccttc cggtttgacg atcgctgaac ctttcatgtc
     2161 ttttgtcgcc atacgaaccc gaactttgtc gtcgaaggaa aggttggcac ttggtctgag
     2221 aaccacaa
//
LOCUS       DAOQUC010000666         2224 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_61168_length_2224_cov_2.495159, whole genome shotgun sequence.
ACCESSION   DAOQUC010000666 DAOQUC010000000
VERSION     DAOQUC010000666.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2224)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2224)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2224
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61168_length_2224_cov_2.495159"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            3..128
                     /locus_tag="PKH07_11245"
     CDS             3..128
                     /locus_tag="PKH07_11245"
                     /inference="COORDINATES: protein motif:HMM:NF012554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HNT35562.1"
                     /translation="MHASSNEEDAKREISLWFEEDEIASNYERSDQQEHFLSKED"
     gene            191..1066
                     /locus_tag="PKH07_11250"
     CDS             191..1066
                     /locus_tag="PKH07_11250"
                     /inference="COORDINATES: protein motif:HMM:NF014017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HNT35563.1"
                     /translation="MNAIHDAQQDSLFDSLGDHESATRCSVANAQVLIGTSGYSFDDW
                     KGTYYPSSLPNKDMLDYYARDFSIVEINASYYRLLPSKTYESMLDRVPKDFRFVVKAY
                     EGLTHRRELDDSIVRQFEQSVRPLTDAHRLEAVLLQFPWSFRHTPENTDRLIQLVYHF
                     PTLRLAAEFRNNTWAIPQMERLLHDSRVAFCCVDEPRLEGLMPNFSLATRKDLGYVRL
                     HGRNAKHWWKGGALRYDYDYSDQELAEWADKIQSLVKKTGRVLTFFNNCHLGQAVKNA
                     KSLRELLNERGLKVT"
     gene            1253..>2224
                     /locus_tag="PKH07_11255"
     CDS             1253..>2224
                     /locus_tag="PKH07_11255"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35564.1"
                     /translation="MLLSARMLGKLQGIEERYAQLRYQKVAEIPMEFCDTNDYLVCEP
                     VSMKWRRAKPGDLWGKPWTTRWFRGDFQIPVGYKGKKVFIHANVGSHDTLFFVDSAPW
                     GVFDENHPVVLLTKSGVVGKSYHLALEGYSGHSYPGAHPHDNPRPMQPNSRTFSGVEA
                     MLEREDVSAFVFDFRVLRQLQNALDSNSLRKHEIIKCLSKVFEIVDSIPNETDESDWR
                     PKLAEARKAMRPLLECRNGSTTPWFGVIGHSHLYTAWLWPLREARRKAARTFSSVLNL
                     MEQYPEMLFVQNVPCHSDMIAEDYPLLFKRVQSQVRKGRWEPNGPMWV"
BASE COUNT          556 a          566 c          571 g          531 t
ORIGIN      
        1 tcatccatgc ttcctcgaac gaagaggatg caaaacggga gatcagtctc tggtttgagg
       61 aagacgagat tgcctccaac tacgagcgaa gtgaccaaca agaacacttt ctgtctaagg
      121 aagactgatt cttcgacttg cttcatgctg attcgttcca cgagacggtg ctcttatatg
      181 aggtcgcacg atgaacgcaa tccatgacgc ccaacaagat tcgctattcg actcactcgg
      241 cgaccatgag tccgccactc gctgctctgt cgccaacgct caggtactta ttgggacttc
      301 cggttacagt tttgatgact ggaaggggac ttactaccct tcgtcactgc caaacaagga
      361 tatgctcgat tactatgcaa gagacttttc aatcgtggag atcaacgctt cctactaccg
      421 gctcctgcca agcaagacct acgagtcaat gcttgatcgt gttcccaagg atttcaggtt
      481 tgtggtcaaa gcctatgaag ggctgaccca cagaagggag ctggacgact ccattgtcag
      541 gcagtttgag cagtccgtca gacctctgac ggatgcacat cggcttgagg cagtccttct
      601 gcagtttcca tggtcattcc gccacactcc tgagaacaca gaccgcctga tccaattggt
      661 ctatcacttt ccgacgcttc gtctggccgc agaattccga aataatacat gggcgattcc
      721 acagatggaa aggcttcttc atgattcccg tgttgccttt tgttgtgtgg atgagcctcg
      781 tctcgaaggt ttgatgccca acttctcact cgcgacccga aaggacctcg gatacgttcg
      841 tctccacggt cgaaatgcaa agcactggtg gaaaggtgga gcactgcgct atgactatga
      901 ttactccgac caggaacttg ccgaatgggc tgacaagata cagagtctcg tgaagaagac
      961 aggccgagtt ctcacattct tcaacaactg tcatcttgga caggcagtaa agaacgcgaa
     1021 gtcgctccgg gaactgctca atgaaagggg tctgaaggtt acttagccga atctgctctg
     1081 gcagagactg caggagtgcc ttcactgatg gcagaactgc gtgttcaagg tgatagagat
     1141 atcggagctt tgcggcatca gttgtaactg ctttctcctc atgatacctt ctcaaagctt
     1201 gttcagaaat atatcagtct ggggtacccc agaaagtaaa ggattaccaa aaatgttgct
     1261 cagtgctcga atgctcggaa aactccaagg gattgaagaa cgatacgctc agctgcgcta
     1321 ccagaaggtg gccgaaatac ctatggagtt ctgcgatacg aacgactatc tcgtctgtga
     1381 gcctgtgtcc atgaagtgga gaagggctaa gccgggggac ctatggggaa agccttggac
     1441 gacacgttgg tttcgagggg actttcaaat tcccgtagga tacaaaggga agaaggtctt
     1501 catccacgca aacgttggat ctcatgacac gctcttcttc gttgactctg ccccgtgggg
     1561 agtgtttgac gaaaaccatc ctgtcgtact tctgacgaag agtggtgttg ttggcaagtc
     1621 ctatcacctc gctcttgaag ggtattcggg gcactcctat cctggagcgc atccgcatga
     1681 caatcctagg ccgatgcagc ctaactcccg aaccttctct ggtgttgaag ccatgctgga
     1741 gcgggaagat gtatcggcct ttgtgtttga tttccgtgtt ttaaggcaac tgcaaaacgc
     1801 actggacagc aacagcctga gaaaacacga aatcatcaag tgcctgtcaa aggtctttga
     1861 gatcgtagac agcataccca atgaaacgga cgagtctgac tggcggccga aactcgccga
     1921 agcaagaaaa gccatgaggc ctcttcttga atgcagaaat ggttcaacaa cgccttggtt
     1981 tggtgtgatt ggccattcac atctgtacac tgcctggctt tggcctctgc gcgaggcgcg
     2041 acgcaaagca gcacgtacct tctcctcagt actgaacctg atggagcagt atcccgaaat
     2101 gctctttgtc cagaacgtcc cctgtcactc ggacatgatc gcggaagact acccgttact
     2161 gttcaaaaga gttcagagcc aagtgcgcaa gggacgctgg gagcccaacg gccctatgtg
     2221 ggtc
//
LOCUS       DAOQUC010000667         2222 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_61232_length_2222_cov_3.233964, whole genome shotgun sequence.
ACCESSION   DAOQUC010000667 DAOQUC010000000
VERSION     DAOQUC010000667.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2222
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61232_length_2222_cov_3.233964"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1039
                     /locus_tag="PKH07_11260"
     CDS             <1..1039
                     /locus_tag="PKH07_11260"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF012585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HNT35565.1"
                     /translation="LGIDAVIYFGGNDSSDQLLGLSRFAEGVSTMHGIKTIDNDLPVT
                     HHCPGYGSAALFNATAIKNLDSDFSSYRVKANFNIDGKVVQAWDIAPVAIYQVMGRKA
                     GWLAQGTAFAKVDPKGDIDPDKAPHIILSKEVTYDEEEFLTKLDEVITRLGSAVVVVQ
                     EDLTDRKTGKSLAKLHATQVATDQHGNIQHGRATSFSPGTFIAQVINEKLKVKAVCGS
                     VKDAAIIPQHIQRSCMMSSVDASEAFKVGAGCIQALENGQDKKSVILTRSQGVTRVDL
                     TDMSNIAAKERCVPLEFINHLDGPTQEFVNEFIYLIGGPAALPVYSRPLFPAVSIPKS
                     ITENPYVANKK"
     gene            complement(1098..1925)
                     /locus_tag="PKH07_11265"
     CDS             complement(1098..1925)
                     /locus_tag="PKH07_11265"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HNT35566.1"
                     /translation="MRHRTLVAAVVSFGESLRTLSSGIVVVTLLGVVLCGCGSPEPLS
                     IEGMVFIPAGSFIMGNDDGDPDEIPAHKVRLHSYYIAEHEVTNEQYGTFVKATGYRPP
                     KGWEHPGFSDPKAPVAGVSWIDAVKYCQWLAQETERPYRLPTEAEWEKAARGTREFVF
                     PWGDFWDPSRCNWGSEPVGYMRHTVPVGSFESGKSPYGVYDMAGNVWEWCWDWYDPGY
                     YQFSDKVSPQGAFTGTQKVARGGCWNDNSRYCRTTDRWPVEMTCRELYVGFRVCLSK"
     gene            complement(1915..>2222)
                     /locus_tag="PKH07_11270"
     CDS             complement(1915..>2222)
                     /locus_tag="PKH07_11270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HNT35567.1"
                     /translation="GDAKMKVQAIEDVDTRVLGRILEKLPKPYRILILPDHPTPVPLK
                     THSTDPVPFLIYDSSWSEARGSERFCERTAQSRGFWIKEGFRLMGQFLRMGTLRNAP"
BASE COUNT          511 a          639 c          577 g          495 t
ORIGIN      
        1 tctgggaatt gatgcggtta tctacttcgg tggcaatgat tcctcggatc agctgctcgg
       61 tctaagccgt tttgccgaag gcgtttcgac gatgcatggc atcaagacca ttgacaacga
      121 tctgcccgtg acccaccact gcccgggtta cggttctgcc gccctgttca acgcgacggc
      181 gatcaagaat ctggacagcg atttttcttc gtatcgcgtg aaagcaaact tcaacattga
      241 tggcaaagtc gttcaggcgt gggacatcgc tccagtggca atctaccagg tcatgggtcg
      301 aaaggccgga tggctcgccc agggaactgc atttgcgaaa gtagatccca agggcgatat
      361 cgatcccgac aaggcacccc acatcattct gagcaaagaa gtgacctacg acgaagaaga
      421 gtttctcacg aagttggatg aggtcatcac tcgactgggc tctgcggtcg tcgtcgttca
      481 ggaagatctg actgatcgca agaccggcaa gtcgctcgcc aaactgcacg caactcaggt
      541 cgccaccgat cagcatggca acatccagca tggacgcgca acgtccttct cgccggggac
      601 ctttatcgcc caggtcatca atgagaagct caaggtcaaa gcggtttgtg gttcggtcaa
      661 agacgccgcc atcattccgc aacatattca gagaagctgc atgatgtctt ccgtggatgc
      721 ctcggaagcc ttcaaggtgg gtgccggctg cattcaggca ttggagaatg gacaggacaa
      781 gaagtccgtc atcctcaccc gcagtcaggg agttacccga gtggatctga cggatatgtc
      841 aaacatcgcc gcgaaagagc gctgcgtgcc cctggagttc atcaatcacc ttgacggtcc
      901 aacccaggag tttgtcaacg agttcatcta tctcattggt ggacccgctg cgctgccggt
      961 ctattcgaga ccgctcttcc cggccgtgtc tatcccaaag agcatcactg aaaaccccta
     1021 cgtcgccaac aagaagtaga cagacttcac acactgaaca acacaagagc cggcaggact
     1081 atcttgccgg ctcttaccta tttcgacaaa cacactcgaa accccacgta gagttctctg
     1141 caggtcatct cgactggcca gcggtcggtt gttcgacaat agcgggagtt gtcattccag
     1201 cagccgccac gggcgacttt ttgtgttccc gtgaatgcgc cttgtgggct gaccttgtcg
     1261 ctgaactggt agtagccggg atcataccag tcccagcacc attcccagac gttgccagcc
     1321 atgtcataga cgccgtaggg actctttccg gattcgaatg accccacggg aacggtatgc
     1381 ctcatgtatc cgacgggttc cgatccccaa ttgcagcggg agggatccca gaaatcaccc
     1441 cagggaaaga cgaactctcg agtgcctcgt gctgcctttt cccattccgc ctctgttgga
     1501 aggcgatacg gtctttctgt ctcctgtgcc agccattggc aatacttcac tgcgtcaatc
     1561 caggaaactc ctgccacagg ggctttcgga tcactgaatc caggatgctc ccatcctttg
     1621 ggaggacggt atcctgttgc cttgacaaaa gtgccgtatt gctcgttggt gacttcgtgt
     1681 tctgcaatgt agtaactgtg gagtctgacc ttgtgcgctg ggatttcgtc aggatctccg
     1741 tcgtcgttgc ccatgatgaa actgcccgcg ggaatgaaca ccatgccttc aatcgagaga
     1801 ggctcaggac tgccacatcc gcacagaaca acgcccagca gagtcacaac gacaatgcct
     1861 gatgacagag tccgcagact ctccccgaaa ctgacaacag ccgcgaccag agtcctatgg
     1921 cgcattgcga agcgtcccca tgcgcaggaa ttgacccatg agccgaaagc cttctttgat
     1981 ccagaagccc cggctctgtg ccgtgcgctc gcaaaaacgc tctgagcctc gtgcttctga
     2041 ccaggaactg tcatagatga ggaacggaac cggatccgtt gagtgtgtct tcagagggac
     2101 tggcgtcgga tgatccggca gaatcagtat tcggtaaggc ttgggaagtt tctcaagaat
     2161 tcttcccaag actctcgtat ccacatcttc aatggcttga accttcatct tggcatctcc
     2221 ca
//
LOCUS       DAOQUC010000668         2219 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_61368_length_2219_cov_3.223660, whole genome shotgun sequence.
ACCESSION   DAOQUC010000668 DAOQUC010000000
VERSION     DAOQUC010000668.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2219
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61368_length_2219_cov_3.223660"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..361
                     /locus_tag="PKH07_11275"
     CDS             <1..361
                     /locus_tag="PKH07_11275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35568.1"
                     /translation="RVNLQPENHVIKALLESVGLSLEPQPNVGTLLVLNMKQLMERLR
                     PYFETRVGIQCAKAIVVKELDGDVFVFTDGQDELRVQGLQNLGDFVFGVSRERVAGSV
                     WSKVFPVPGLWYGLNYI"
     gene            380..1384
                     /locus_tag="PKH07_11280"
     CDS             380..1384
                     /locus_tag="PKH07_11280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007914119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HNT35569.1"
                     /translation="MPSSELKGMYLLNKGSFDVIYGEQERQDIAERVSIAAPLHTAES
                     VNADPSVLSEIDIIFSGWGMAKLDESLLGQAPRLKAVFYGAGSVKGFVSEAFWERGIL
                     LTSAYAANAVPVCEYTLSQILFCLKHGWQYALAIKREGKYPTRAPVPGGYQSTVGIIS
                     LGMIGRGMCEMLNRFDLNVLAYDPFATDEDAKALNVQLCSLDEIFVRSDVVSLHAPRL
                     KETEGMITGAHLESMKPGSTFINTSRGAIVRESEMIEVLKRRPDLFAVLDVTNPEPPQ
                     PGSDLYTLPNVIVTPHIAGSMSGECRRMGRYMVEELDRFLRGEPLKWQITREKVARMA
                     "
     gene            1426..2028
                     /locus_tag="PKH07_11285"
     CDS             1426..2028
                     /locus_tag="PKH07_11285"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HNT35570.1"
                     /translation="MDSILRVLGIGGSPRRGGNSEILLDEALRTAVALGASAEKIVLN
                     DLSIAPCQECSGCDATGRCIVQDEMQDIYPRLRCADVMVLATPVFFSGPSAQLKAMID
                     RTQACWVERFVLKRPVSDTARMRRGLLLSVGARGGEANFQAVEKIVRTFFYTQSVKYQ
                     DGLFFPNVDHKAGILSVDGVMSRVQDAVQLTLSEVVAGSR"
     gene            complement(2036..>2219)
                     /locus_tag="PKH07_11290"
     CDS             complement(2036..>2219)
                     /locus_tag="PKH07_11290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014956521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNT35571.1"
                     /translation="IRALKGLLPICSKCKKIRDDTGYWQQVEVYIRDHSDAEFTHALC
                     PECVKELYGDPKDPKF"
BASE COUNT          482 a          519 c          644 g          574 t
ORIGIN      
        1 cagagtgaac ttgcagccgg aaaaccatgt gattaaggcg ctgctcgaaa gtgttggtct
       61 gagtcttgaa cctcagccga atgttggaac actccttgtg cttaacatga agcagctgat
      121 ggaacgtctc cgtccttatt ttgagacacg agtcggtatc cagtgcgcga aggccattgt
      181 tgtcaaggaa ctcgatggag atgtctttgt attcactgac ggccaggatg aacttcgtgt
      241 ccaaggactt cagaatctgg gcgattttgt ctttggtgtt tccagggaac gtgtagcagg
      301 aagtgtttgg agcaaggtat tcccagtccc cgggctctgg tatggactta actacatctg
      361 aacaaatgga ggttgaaaga tgccctcatc cgaactgaag ggtatgtacc tgttgaacaa
      421 aggatccttt gatgtcatct atggtgaaca ggaacgtcag gatatcgcgg agagggtgtc
      481 cattgctgct cccttgcaca cggcggaatc tgtcaacgcc gatccttctg ttctttcaga
      541 gattgacatc atcttctcgg gttggggcat ggcaaaactc gatgagtcgc tgttgggaca
      601 agcaccccga ctgaaggccg tgttctacgg agccggttct gttaaagggt ttgtgtctga
      661 ggcgttctgg gagcgaggca ttcttctgac aagtgcttat gctgccaacg ccgtgcctgt
      721 ttgtgaatac acactctcgc agattctgtt ctgcctgaag cacggttggc agtatgcact
      781 tgccatcaag cgggaaggga agtatcccac tcgagcgccg gtgcctggcg gctatcagag
      841 cactgtcggg attatctcgc ttgggatgat cgggcgtggc atgtgcgaaa tgctcaacag
      901 attcgatttg aatgtgcttg cctacgatcc gtttgccaca gatgaggatg cgaaagcgct
      961 caacgtccag ttgtgttccc tcgatgagat cttcgtccgt tccgatgtcg tttcacttca
     1021 tgctccgcgg ttgaaagaga cagagggcat gatcacagga gcacacttgg agtccatgaa
     1081 accgggttcg acattcatca acacatcgcg aggtgccatc gttcgtgagt ctgagatgat
     1141 cgaggttcta aaacgtcgtc ccgatctctt tgcagttctt gatgtcacga acccggagcc
     1201 tcctcagcca ggttcggatc tctacaccct tccaaacgtc atcgtgactc ctcacattgc
     1261 cggctcgatg agcggtgaat gccgacgcat gggacgctac atggttgagg aactcgatcg
     1321 gttcttacga ggcgagccat tgaaatggca gatcacccgt gaaaaggttg ctcggatggc
     1381 ctagtgtttg ttgcctgtga atcacaatgt tgaaggagcc ttcatatgga cagtattctg
     1441 cgtgtcctcg ggattggagg aagtccgcgt cgtggtggca actccgagat acttctcgac
     1501 gaagcactca ggacggctgt ggctttgggt gcgtctgctg agaaaatcgt tttgaacgat
     1561 ctgtccatcg caccatgtca ggagtgttct ggttgcgatg caacgggtcg gtgtattgtt
     1621 caggatgaga tgcaggatat ctatccaaga ctccgttgtg cggatgtcat ggtccttgcc
     1681 acaccggtct tcttcagcgg cccaagcgcg cagttgaaag ccatgatcga tcgaactcag
     1741 gcatgctggg tggaacgttt tgttctgaag aggccggtgt cggacacagc tcgaatgagg
     1801 agagggctcc ttctttcagt gggtgcgcgt ggcggggaag ccaactttca agcagttgaa
     1861 aagatcgtga ggactttctt ctatacccag agtgttaagt accaggacgg cctgttcttc
     1921 ccaaacgtag accacaaggc tggaatcctg agtgttgatg gggtgatgag cagagttcag
     1981 gatgccgttc agctgacgct ctcagaagtc gttgccggga gtcggtagag cgttttcaga
     2041 atttgggatc tttggggtcg ccgtagagtt ccttgacgca ttcagggcag agggcatgtg
     2101 tgaattcggc atctgaatga tccctgatgt agacctcaac ctgttgccag tatccggtgt
     2161 catcgcggat tttcttgcac ttgctgcaga tgggcagaag cccctttaga gcgcggata
//
LOCUS       DAOQUC010000669         2216 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_61499_length_2216_cov_2.871356, whole genome shotgun sequence.
ACCESSION   DAOQUC010000669 DAOQUC010000000
VERSION     DAOQUC010000669.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61499_length_2216_cov_2.871356"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..868
                     /locus_tag="PKH07_11295"
     CDS             <1..868
                     /locus_tag="PKH07_11295"
                     /inference="COORDINATES: protein motif:HMM:NF012363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNT35572.1"
                     /translation="GANGCLSSASFAQKAQKQSRRGVGIEPDRIIPYKTIGDVTLSLH
                     VFEPDIRKATPPFSAIVFFFGGGWRTGSPRQFYGQCQYLARRGMVAISAEYRIESTHG
                     TSPFECIADAKSALRFLRANAESFKVDPNRIVASGGSAGGHIAACSGIIPGLESDGEN
                     LDISSVPNALVLFNPVVMLTDGKSNLIGKFSGRPLEASPHHFVREGLPPTIIFHGKED
                     RLIPYWTIEEFARKMQENGNVCQVVGFEGVGHGFFNEASSPDSPNQKTIRLTDEFLVS
                     LGMIPSATRVLH"
     gene            1011..>2216
                     /locus_tag="PKH07_11300"
     CDS             1011..>2216
                     /locus_tag="PKH07_11300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNT35573.1"
                     /translation="MNSTRKRDRFYYTTVISTYLVVAIIYIIWSHLADQEALRTDNDA
                     RLLTAAQSIKFIHTTDFHDRALSSDSVPPDEHQMNTASLFGFAREIGSPTLYTLVERQ
                     GRLYYTSSSSIRMGRDKLPQRPYFEPFPDPPEPLLEALERSQPLVFSQSKDAREYRIA
                     TVPELSPQGRRYLSCATASMDALRSSLRHQLLQTILGSIALLLVAFPMLLLHRRLFIS
                     HEKDLQSLNSQLSKDILERSKIEKELAHERYLLQELMENIPDNIYFKDAESRFIRINS
                     TLAAKCGLSDPSEAIGKNDFDFFAEEHARQAFEDEQEIIKTGKPLASILEKEVWLDGR
                     VTWVSTTKVPLRTQEGKIVGIVGISRDVTDWKQADDALKLANRRLQMIAESSAMFLAK
                     ASLRQQSQQL"
BASE COUNT          539 a          588 c          544 g          545 t
ORIGIN      
        1 cggcgcgaat ggttgtcttt cgagtgcgtc ttttgcgcag aaggctcaga aacagtcccg
       61 gaggggtgtt gggattgagc cagacaggat tattccctat aagaccattg gcgacgtaac
      121 actctctttg catgttttcg aaccggatat ccggaaggcg acgccaccct tttccgcaat
      181 cgtcttcttc tttggtggag gctggagaac gggatcgcct cgtcagttct acggacagtg
      241 ccagtacctc gctcgacgag gtatggtcgc catctctgct gagtaccgca ttgagtccac
      301 acatggcacg tcacccttcg agtgcatcgc cgatgccaaa tccgctcttc gctttcttcg
      361 tgccaatgcg gaatccttta aggtggatcc caatcgaatc gttgcatctg gtggatcagc
      421 aggtggacat atagcggcgt gttctggaat catccctggc ctggaaagtg atggcgagaa
      481 tctcgatatt tcgtccgttc caaatgcctt ggttcttttc aatccggtcg taatgcttac
      541 agacggcaag tcgaatctta tcgggaagtt ttccgggaga ccccttgaag cgtcacctca
      601 ccattttgtg cgtgagggac ttcctccgac aataatcttc catggaaagg aagaccgact
      661 cataccctat tggactattg aggaattcgc caggaagatg caggagaatg gcaatgtttg
      721 ccaggtagtc ggctttgaag gtgtcggtca cggattcttc aatgaagcgt caagtccaga
      781 cagcccaaat cagaagacca tcagactcac cgatgaattt cttgtctccc tggggatgat
      841 cccgagtgca actcgggtct tacattgacc agcacaacac ttgatttctc agactgttgg
      901 cagagatcga gcagattgat ggtatgcaat tgcgtgcagg gaaacatttc tcttctcaac
      961 cggagtatct ccagtataat gacttgtgtc tgaacgaggt ttctcatgca atgaacagca
     1021 cacgcaagcg agacagattc tattatacca cggtcatttc gacctatctc gtggttgcga
     1081 tcatctacat tatctggtct catctggctg accaggaagc tctgcgcaca gacaacgatg
     1141 caagactcct tacggcggca cagagtatca agttcattca tacgacagac ttccatgatc
     1201 gtgctctctc ctcggattcc gtgccccctg acgaacatca gatgaacacc gccagtttgt
     1261 ttggctttgc ccgggagatc ggatctccaa cactctatac acttgtcgag aggcaaggac
     1321 gcttgtacta cacgtcaagc agttccatca gaatgggtcg agacaagttg cctcagagac
     1381 cctactttga accattcccc gatccgcctg aacccctact cgaagcactc gagagaagtc
     1441 agcctcttgt attcagtcag tccaaggatg ctcgagagta ccgcatcgct acagttcctg
     1501 agttgtctcc tcaaggaagg cgatatctct cctgtgccac tgcctcgatg gatgcactca
     1561 ggtcttcgtt gagacatcag cttctacaga cgattctcgg ttccatcgct ctcttgcttg
     1621 ttgccttccc gatgctgctg ctccatcggc gtcttttcat ctcgcatgaa aaggatcttc
     1681 aatctctgaa cagtcagctg agcaaggata ttctggagcg ctccaagatc gagaaggaac
     1741 tggcacatga aagatatctg cttcaggagt tgatggagaa catacctgat aacatctact
     1801 tcaaggacgc cgaaagccgc ttcatccgca tcaacagcac actcgccgcc aagtgtggcc
     1861 tgagtgaccc atcggaagca atcgggaaga atgatttcga tttctttgca gaggagcacg
     1921 cacgccaggc attcgaagat gaacaggaga tcatcaagac cggaaagcca ctggccagca
     1981 tcctggaaaa ggaagtctgg ctcgacggca gagtcacctg ggtctcaaca accaaagtcc
     2041 ctctccgaac tcaggagggc aaaatcgttg gcatcgttgg aatctccaga gatgtgaccg
     2101 actggaaaca ggctgacgat gcgttgaagt tagccaacag acgtctccag atgattgccg
     2161 aatcctctgc catgtttctc gccaaggctt cattgaggca acagtctcag caactt
//
LOCUS       DAOQUC010000670         2213 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_61612_length_2213_cov_4.562095, whole genome shotgun sequence.
ACCESSION   DAOQUC010000670 DAOQUC010000000
VERSION     DAOQUC010000670.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2213
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61612_length_2213_cov_4.562095"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            366..1172
                     /locus_tag="PKH07_11305"
     CDS             366..1172
                     /locus_tag="PKH07_11305"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNT35574.1"
                     /translation="MWKSLYRNRSVLWSFVARDLRSRYAGTSMGLVWTLLNPILQILI
                     YVFVFSSILSSKFGLLRWGEIPYWIYLCSGLLPWIAFQEINARSTTLYLENSNLIKKV
                     FFPAEVLPAYLTVSGLINLTISMSFLLLVLAVSGQFPGWAVAFLPVLMLLQHLACFGL
                     AMALSVLSVFFRDLIQLVQLVLMVWFWATPICYPEDIVIGSSSFPTWLQAVYRLNPFL
                     SLVNGYRNIFLKGQIPYWQDMAMALAFGLAAFLVGKLLFRTQMKEIVDLV"
     gene            1198..>2213
                     /locus_tag="PKH07_11310"
     CDS             1198..>2213
                     /locus_tag="PKH07_11310"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNT35575.1"
                     /translation="MSAVVANNISKKYKLYDRPSDRLKEWFSLPRQTCHRDFWALKDV
                     SFELPKGSALGVIGPNGAGKSTLLKILTGTTVPTEGTYSLIGRVASLLELGAGFHPEF
                     TGRQNLQMNARMLGLTDDEIRERMDEIIEFAELKAFIDQPVRTYSSGMYVRLGFAIAS
                     SVRPDVLIVDEALSVGDAYFQRKSIDRIQYFREHGVTILFVSHDLSIVRRFCDQALWL
                     NEGRVQNFSDVHRVSKAYELWERKKEQVSLGIEYQMEQTAAEPELDGSGVTSREQKKK
                     LRVLGSTWGSGEVKITRVEMLNTEGKSGWTFTTGEPITIRYHFYAFKEIPTPVFGLNI
                     HRLDG"
BASE COUNT          464 a          550 c          607 g          592 t
ORIGIN      
        1 ctcctcgttg acggccctgg gatcgccgag cgtctgctcg gcaactcctt ctcccaaggg
       61 agaaggtcgg gatgaggagc tccgagattc ttcgcatagc ctccgatgac aagaacacgt
      121 agggcggcat tcatgctgcg cgggacaaat cccgccctac tgctttcatg ataccttgag
      181 gatatctaac tctccactgc ccgtcaaatt atcatcctcc caacactttt tgcctctcca
      241 gttaggaatt cgtgttagca tgcttaagcc gtcggatatc ccgttcgggg gattccgcgg
      301 cgcagaaatc ccttagggaa acacggcagc gttctgcctc gtttcgatga agaattcttt
      361 ggaaaatgtg gaagagtctc tatcgtaatc ggtcggtgct ctggagtttt gtcgcgcgcg
      421 atctgcggag tcgttacgcc ggtaccagca tgggtctggt ttggacgcta ctcaatccca
      481 tactccagat tctgatctat gtgtttgtat tctcctcgat cctgagcagc aagtttggac
      541 tgctccgttg gggggagatt ccgtactgga tttatctgtg ttcgggactc ttaccctgga
      601 tcgcctttca ggaaatcaat gcccgctcga caacccttta tctggagaat tccaatctca
      661 tcaagaaagt cttcttcccc gcggaagttt tgcccgctta cctgactgtt tctggcttga
      721 tcaatctcac gatcagcatg agttttctgc tgttggtgtt ggctgtctcg ggtcagtttc
      781 ccgggtgggc agtggcgttc cttccagtgc tgatgctgct tcagcatctg gcctgttttg
      841 gactggcgat ggcgctgtcg gttctgagcg tatttttccg agatctcata caactggtgc
      901 aactcgtgtt gatggtttgg ttctgggcga ctccgatctg ctaccccgag gatatcgtta
      961 tcgggtcgtc gagttttccg acgtggcttc aggcggtcta tcgcctgaat ccgttcctga
     1021 gtctggtaaa cggttatcga aacatttttc tgaagggaca gattccctat tggcaggaca
     1081 tggcgatggc gttggctttc ggccttgccg cgtttcttgt cggaaaattg ctgtttcgaa
     1141 cgcaaatgaa ggagatcgtt gacctggtct gaggtcaaag ggaaaagaga aggcgtgatg
     1201 tctgcggttg ttgcaaacaa catttccaag aagtacaaac tctacgatcg gccttccgat
     1261 cggctcaagg aatggttctc cctgccacgc cagacatgcc atcgcgattt ctgggcgctc
     1321 aaggatgtca gttttgaact gcccaagggc agcgcgttgg gagtcatcgg ccccaatgga
     1381 gcgggcaagt ctacactcct gaaaattctc accggaacaa ccgtccccac cgaaggaacg
     1441 tattctctga tcggtcgtgt ggcttcactt ctggaactgg gagccggttt tcatcctgaa
     1501 ttcactggac gacagaatct tcagatgaac gctcgaatgc tcggactgac tgatgatgag
     1561 attcgagagc gcatggacga aatcatcgaa ttcgctgaat tgaaggcgtt cattgaccag
     1621 ccggtacgca cctattcatc gggtatgtat gttcggcttg gctttgccat tgcttcgagt
     1681 gtgaggccgg acgtgctcat tgtggatgag gcgttatcgg tcggagacgc ttattttcaa
     1741 cgtaaatcaa tcgatcggat tcagtatttt cgggaacacg gggtgacgat cctgttcgtc
     1801 tcccatgatt tgagcatcgt gcgacgtttc tgtgatcagg cactgtggct taacgaaggg
     1861 cgtgtgcaga atttcagtga cgtgcatcgt gtttccaaag cctatgagct ttgggagcgc
     1921 aagaaggaac aggtcagttt gggcatcgag tatcagatgg aacaaaccgc tgctgagcct
     1981 gagctcgacg gttccggagt cacgtcccgt gaacagaaaa agaagcttcg ggtgctgggt
     2041 tcaacctggg gttccggcga ggtgaagatc acacgggtgg agatgctcaa caccgaaggc
     2101 aagagcgggt ggacgtttac caccggagag ccgatcacca ttcgatatca tttttacgct
     2161 ttcaaagaaa ttccaacacc tgtcttcggt ctgaacatcc atcgtctgga cgg
//
LOCUS       DAOQUC010000671         2205 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_61994_length_2205_cov_2.968372, whole genome shotgun sequence.
ACCESSION   DAOQUC010000671 DAOQUC010000000
VERSION     DAOQUC010000671.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61994_length_2205_cov_2.968372"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..555)
                     /locus_tag="PKH07_11315"
     CDS             complement(<1..555)
                     /locus_tag="PKH07_11315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35576.1"
                     /translation="MKHNHTLSFSLLNIFVVLSVTSPVSQSAEITVQNTLPRTDVDGR
                     IIDAHDGCLEYFDGRFYLYGTRYGETDGFTTANRYVCYSSDDLSHWTFHGEILQDPPE
                     GVYYRPYVKYNPKTRKYVLWYNWYPRLWEGHYGVATSDSPRGLFVIENSDVSVTRPRP
                     GDHGLMVDDDGKGYIIYTSIAEGHG"
     gene            complement(619..1020)
                     /gene="tatB"
                     /locus_tag="PKH07_11320"
     CDS             complement(619..1020)
                     /gene="tatB"
                     /locus_tag="PKH07_11320"
                     /inference="COORDINATES: protein motif:HMM:TIGR01410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HNT35577.1"
                     /translation="MNIGISELAVVFVLALLIFGPKKLPEIARGLGKAMRMFREASNE
                     IQRTLEEDIRAEENRKLLSELKRDVSTTMNQIEADLEDEDFDESDDSDESYEQYEDSR
                     EPTTPEQAAPSDQTTSQQASVDTEQPKDLAG"
     gene            complement(1379..>2205)
                     /locus_tag="PKH07_11325"
     CDS             complement(1379..>2205)
                     /locus_tag="PKH07_11325"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF002872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HNT35578.1"
                     /translation="EFKELKISDVGGIINRGGTILLSARCAEMYQQEGQEKAAGILKE
                     LGVDALVAVGGDGTYRGGHAIHKFSGIPVIGVPGTIDNDISGTDFTIGFATAVNTALD
                     AIDKIRDTATSHERLFVVEVMGRHAGFIALETAIAGGAEAVLMPEVPYDLVEIFTRIE
                     EGTKRGKLSSIVVVAEGAGSSMEIAYRIEKTLGLDVRVAVIGHLQRGGIPVAPDRVLA
                     ARLGKAAVDVLLQGETGAKMVGVISGKISVHDLEHCWTHTKKIDAEMVDLARVLAI"
BASE COUNT          492 a          625 c          568 g          520 t
ORIGIN      
        1 gccgtgccct tcagcgatgg atgtgtagat gatgtacccc tttccgtcat cgtccaccat
       61 caagccatgg tcacccgggc gtggtcgtgt caccgaaaca tcggagttct cgatcacgaa
      121 caacccccgt ggggaatcag atgtggcaac tccatagtgt ccttcccaaa gtctcggata
      181 ccagttgtac cagagaacat actttcgtgt cttgggattg tacttcacat aggggcgata
      241 gtagacgccc tcgggcgggt cttgaagaat ctccccgtga aatgtccaat gactcagatc
      301 gtcagaactg taacagacgt atcggttcgc cgtagtgaaa ccgtccgtct ctccgtatct
      361 cgttccatag aggtagaaac gaccatcgaa gtattcaaga caaccgtcgt gagcatcaat
      421 gatcctgccg tcaacatctg ttctcggaag tgtgttttga acagtgatct ctgcgctttg
      481 cgaaaccggg gaagtcacag acaggacaac gaatatgttg agaagagaga acgagagagt
      541 atggttgtgc ttcatcaaat gccagccttg ctgagttgaa atgctacgcc caaacgcctg
      601 cagttttgtg gattttggct atccggcaag atcctttggt tgctctgtgt ccacggaggc
      661 ttgctgactt gtggtttgat cagagggcgc tgcttgttca ggggtagtcg gttcgcgcga
      721 atcctcgtac tgttcgtaag actcgtcgga gtcgtccgac tcatcgaagt cctcatcttc
      781 cagatcagcc tcgatctggt tcatcgttgt agacacatcc cgcttcagtt cgctgaggag
      841 ttttcgattt tcctcggcgc ggatatcttc ttcgagtgtc cgctgaattt cgttggatgc
      901 ctcgcgaaac atgcgcatgg ctttgccaag cccacgggca atctcgggaa gcttctttgg
      961 cccgaagatc aagagggcaa ggacaaagac aacggcaagt tcagaaatac cgatattcaa
     1021 atgagatcac ccaccctgcc gacaggcgtt tctgcacaag cgccagtaaa atgttctgaa
     1081 agaaacgcta cattccacag aggaagagcc ccgacgggat tgcggttgca cgaatccccc
     1141 ctacccccct taccaagggg ggattgcaga ggccaacgcg cgttctgagc agtggctggg
     1201 ttacaaccct tcctaccccc cttaccaagg ggggattgca gaggccaacg cgcgttctga
     1261 gcagtggctg ggttacaacc cttcctaccc cccttaccaa ggggggatag aggggggatt
     1321 atgagaacgg aaaaccggct gtcttgcata ctatgctact cgctttagga atgctctcct
     1381 agatcgccag cactctggca agatccacca tctcggcatc aatcttcttt gtatgtgtcc
     1441 agcagtgttc caagtcatgg acgctgatct taccgcttat gacgcccacc atcttggcac
     1501 cggtctctcc ttgaagaagc acgtccacgg ctgccttgcc cagacgcgct gcaagcacgc
     1561 gatccggagc gactggaatc ccgccacgct gcagatgtcc gataacggcg actcgaacgt
     1621 ccaaccccag cgtcttctca atgcggtacg cgatttccat tgaagagccg gcaccctcgg
     1681 cgacgaccac aatgctggaa agcttccctc gcttggtgcc ttcttcgatt cgcgtgaaga
     1741 tctccaccag gtcgtaaggg acttccggca tcaacaccgc ttctgctccg ccggcaatcg
     1801 ccgtctccaa agcgataaaa ccggcatggc gtcccatcac ttccaccaca aacagcctct
     1861 catgggaagt ggctgtatcg cgaattttgt caatggcgtc caaggcggta ttgactgccg
     1921 ttgcgaatcc gatggtgaaa tccgttccgg agatgtcgtt gtcaatggtg ccggggacac
     1981 cgatgaccgg aataccactg aacttgtgga ttgcatgtcc accgcgatag gtgccgtctc
     2041 caccgacggc gaccaatgcg tctactccga gctctttgag tattccggct gccttttcct
     2101 ggccttcctg ttggtacatc tcagcgcagc gagcactgag aaggatggtg ccaccacgat
     2161 tgatgatccc accgacatca gagatcttga gttccttgaa ttctc
//
LOCUS       DAOQUC010000672         2203 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_62088_length_2203_cov_2.639199, whole genome shotgun sequence.
ACCESSION   DAOQUC010000672 DAOQUC010000000
VERSION     DAOQUC010000672.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2203
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62088_length_2203_cov_2.639199"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..429)
                     /locus_tag="PKH07_11330"
     CDS             complement(<1..429)
                     /locus_tag="PKH07_11330"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35579.1"
                     /translation="MKIRSSRRFSRRILRKTLGVLVPPIAWSIRLVPFPAMLWVFEHL
                     APTVFLLFRNRRNRILENLQRALGSERDPRELRIIARDAVRNYARSLVELIYLNEPSK
                     LFRYVDVRIEGENNLRDGLAQKRGVIVVTAHFGNWELPMLA"
     gene            855..>2203
                     /locus_tag="PKH07_11335"
     CDS             855..>2203
                     /locus_tag="PKH07_11335"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HNT35580.1"
                     /translation="MLRLTPPESIAAKLSEESIQDSDILLCTHTDINHFGRYEPQWLV
                     VTKNALLLLEEGEPAQLIFRMSLSDASEYRCRAVIGSGILEARVDGMYVDILRYSNRF
                     ADRFERIAKKLDRHLHGEDIRIAEEDGSDSRRCPKCQMMLQFAGDVCPRCVDKGVVLS
                     RMWSLMKPYRMAAVGIMALLVLGICLDLVGPQLTRYMVDHVLPGSPEAAEKFQNDPDL
                     TAAHVVMLIEVVFVLAGVQILRMVINIFNGRLGSKIGTSITFDMRGRLVKHLQQLSVA
                     YYDRQQVGSLVGRVAYDTESLHGFVNQLTGGFLFQVIMLLGVGVMMFHINTRLALYTL
                     IPAPLVTAGSIIFWKHIYPRYYRFWDASSKQAGMLSGILSGIRVVKSFGQEPRETERF
                     TQASGRLRGNRLGVDYAASTFNPIMGLVFQFGGWIVWFVGGRDVLSGTMTLGELMAY"
BASE COUNT          501 a          528 c          592 g          582 t
ORIGIN      
        1 cgccagcatg ggcaattccc aattcccgaa atgcgcagtc acaacgatca ctcccctttt
       61 ctgtgccagg ccgtctctca gattgttttc accttcgata cggacatcca cataacgaaa
      121 cagttttgag ggttcattga gatagataag ctcgaccaga ctgcgtgcat agttgcgaac
      181 tgcatcccgt gcaatgatcc taagttctct ggggtctctc tcactcccta gtgcgcgctg
      241 gaggttttca agaatgcggt ttcgcctgtt tctgaagagg aggaaaacgg ttggtgccag
      301 atgctcaaag acccagagca tagcaggaaa tggaacaaga cggatcgacc aggcaattgg
      361 aggtacgagc acccctaatg ttttgcgcag aatccttctg gaaaaccgtc gtgatgatcg
      421 tatcttcaag agtagcagta tctttcgctc tgtctgttct ttccaaagac agtgcaaatc
      481 tacatgaaat cactaaagcc ttcaagaaca taaatcgcag aagaaagaaa cagattgtga
      541 cggtatatga gcgccatgag aaccgatccc ggcatagttg acgccgtgct gagatccttc
      601 gacggtgcga gtaagggaat cgcctaacta cgtctaacct gagggtccct ggtcacaggt
      661 gttttttttg gttgcaaaaa gtgttacgat gcactagaga tcattcgtta gtcagatgtt
      721 ttctaaaggc gctgttgacg ggtttttgga aaaagatgga agaagagcct tctcgagggg
      781 cttgtgcgat agagacattc cgaggaatca ttcccacagt tgtgacaaaa tgatgtgagg
      841 tcgaaggagt ttttatgctt cgcttaactc ccccagagtc cattgcagca aaactttctg
      901 aggagtccat ccaggactct gacattctgc tctgcaccca cacggatatc aatcactttg
      961 gtcgctacga gccacagtgg cttgtcgtca ccaagaacgc gcttctgttg cttgaggaag
     1021 gcgaaccggc gcagctgatt ttcagaatga gcctttccga tgcaagtgaa taccgatgtc
     1081 gcgccgtgat tggctcagga attcttgagg ctcgtgtaga tgggatgtac gtagacatcc
     1141 tgcgctacag caaccgtttt gcggatcgtt ttgagcgcat cgccaagaaa cttgatcggc
     1201 atcttcatgg tgaggacatc cgcattgccg aggaagatgg aagcgactcg cgacgatgtc
     1261 cgaaatgtca gatgatgctt caatttgccg gagacgtctg tcctcgttgt gtggacaagg
     1321 gtgtcgttct gtcccgaatg tggagcctga tgaagcccta ccggatggcc gcagtcggga
     1381 ttatggctct tttggttctg ggcatttgcc tggacctggt tggacctcaa ctgactcgtt
     1441 acatggttga tcatgtcttg ccgggaagtc cagaggctgc agagaagttc cagaatgatc
     1501 cggatctgac ggctgcacat gtcgtgatgc ttatcgaagt tgtctttgtt ttggctggcg
     1561 ttcagattct gcgcatggtg atcaacatct tcaacggccg tttgggcagc aagataggaa
     1621 cctcaattac tttcgacatg cgtggacgtt tggtcaagca cttgcagcag ctttcggtcg
     1681 cctactatga tcgccaacaa gtgggctcac tggtagggcg tgtcgcatat gacaccgagt
     1741 ctcttcatgg ttttgtcaac cagcttaccg gtggattcct gtttcaggtg atcatgctcc
     1801 tcggggtcgg ggtaatgatg ttccacatca acacgaggtt ggctctgtac accttgatcc
     1861 ctgcgcctct ggtcaccgcc ggtagcatta tcttctggaa gcacatctat ccccggtact
     1921 accgtttctg ggatgcttcc agcaagcagg ctgggatgct ttcgggcatt ctctccggta
     1981 tccgagttgt caaatccttt ggacaggagc cacgtgaaac ggaacggttc acccaggcca
     2041 gtggtcgtct tcgaggcaac cgattgggtg tggactacgc cgcttcgaca ttcaacccca
     2101 tcatgggtct ggtgtttcaa ttcggagggt ggatcgtctg gtttgttggt ggtcgagacg
     2161 tgctgtcagg aaccatgacc ttgggagaac ttatggctta ttt
//
LOCUS       DAOQUC010000673         2199 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_62235_length_2199_cov_4.395989, whole genome shotgun sequence.
ACCESSION   DAOQUC010000673 DAOQUC010000000
VERSION     DAOQUC010000673.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2199)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2199)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2199
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62235_length_2199_cov_4.395989"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1003
                     /locus_tag="PKH07_11340"
     CDS             <1..1003
                     /locus_tag="PKH07_11340"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNT35581.1"
                     /translation="YEDAERIHATIPAIEVMVPAKVIRSDVMYRTKRVDADVYGTVPW
                     YPQTANKKILKGRFFTQKEALDGRNVCVLTNDLVKKLFTFEDPIGSRIRIRGEYFSVI
                     GIIDEPVVAASGNSKAGNSNLNLFIPMTAARHRFGDLIMIYRAGSRSVEEVDLHKIIV
                     KVDDVSKVMQTWEVIKDLLARFHKQQDYEIVVPLGLLREAARTKRIYNIVLGSIAAIS
                     LLVGGIGIMNIMLASVTERTREIGIRRALGARQKDIIIQFLTETVLLSTGGGLIGLML
                     GLAIPFLVTHFAGMMTIVRLWSLIVALGISILIGLIFGIYPAYRAAMMNPIEALRHE"
     gene            complement(1049..>2199)
                     /locus_tag="PKH07_11345"
     CDS             complement(1049..>2199)
                     /locus_tag="PKH07_11345"
                     /inference="COORDINATES: protein motif:HMM:NF021379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2079 domain-containing protein"
                     /protein_id="HNT35582.1"
                     /translation="VPLYLIARRRLGGHTIPCLLSICYLLYPAIQGANIRDFHEIPLA
                     TPLLLFLLLMLETGQKRLFWISLVLTLMVKEDLALSCFALGVYAAISRKNVRVGILTC
                     FVCALYFALCVGVIMPMNDGSPMASRFEGYIAPGFTGLKGMLATLLTNPFFTLYFAFF
                     DANKILFLLQMLGPILFLPLIAGNGLVLLAPGFATALLASDGLHYTIGLHYPATLAPF
                     VFYLTILALERIRPSLRLKLAGLLIVSSLLFNYEFGWFFSKRFTGFEYPRQQYDLFRE
                     ITKSIPDSAGVSASNPLIPHLSRRERIYYFPFVGDSDYIVFCTASVGNNFWPETRESS
                     FDAIISIVRRGVFGVVRFEGDFLLLRRGAPANRNPEAVHRLLAIKIQH"
BASE COUNT          584 a          540 c          598 g          477 t
ORIGIN      
        1 atacgaagat gccgagcgaa tccatgccac gattcctgcc atcgaagtca tggttccagc
       61 gaaggtgatc cgttccgatg taatgtaccg caccaagcga gtggacgccg atgtctacgg
      121 gacggtgccc tggtatcccc aaacggcaaa caagaagatt ttgaaggggc gctttttcac
      181 acagaaggaa gccttggacg gtcgaaacgt atgcgtgttg acgaatgatt tggtcaagaa
      241 actgttcact tttgaagatc cgattggctc cagaattcgc attcgaggag agtatttttc
      301 ggtcatcggg atcattgatg agcctgtggt ggcagcctcg ggaaactcca aggcgggtaa
      361 ctccaatctc aatttgttca ttccgatgac cgcggcacgt catcgttttg gtgatcttat
      421 catgatctat cgcgcaggca gtcgctccgt ggaggaagtg gatcttcaca agatcattgt
      481 caaagtggac gacgtcagca aggtcatgca gacatgggaa gtcatcaagg atttgctggc
      541 gcgattccac aaacagcaag actatgaaat cgtcgtcccg ctggggctgc ttcgcgaggc
      601 ggctcgcaca aaacgcatct acaatatcgt tcttggttca atcgccgcga tctcgttgct
      661 cgtcggcggc atcggaatca tgaacatcat gcttgccagc gtcaccgagc gcacacgcga
      721 gatcggcatc cgccgtgcac tgggagcacg acaaaaggac atcatcatcc agtttctcac
      781 tgaaacggtg ctgctttcga ccggaggggg tctcatcggg ctgatgttgg gtctcgcgat
      841 tccgttcctg gtaacccact ttgccggcat gatgaccatt gtccggctct ggtcgttgat
      901 tgttgcattg ggtatttcga tactgatcgg acttatcttc ggcatctacc ccgcttaccg
      961 cgcagcgatg atgaacccca ttgaagcatt aaggcatgag taggaagcgg ggaattcctg
     1021 caagtttcga ttccatcgga tagtcgtctc agtgttgaat cttgatggca agaaggcgat
     1081 gaactgcctc cgggtttctg ttggcaggcg ctcctcgacg cagaaggaga aaatccccct
     1141 cgaatcgaac gacaccaaag acacctcttc gaacgatgga gatgatggcg tcaaacgaag
     1201 actctcttgt ctctggccag aaattgtttc ccacagatgc agtgcagaag actatgtagt
     1261 ccgaatcgcc aacaaaggga aaatagtaga tcctctccct gcgtgaaagg tgaggtatca
     1321 gcgggttgga ggcgctgacc ccagccgaat ccggaatgct cttcgtgatc tctcgaaaca
     1381 aatcatactg ctgccgaggg tactcaaagc cggtgaatcg ctttgagaag aaccagccaa
     1441 actcgtagtt gaaaagcaac gatgagacaa tgagaagtcc agccaatttc aaccgcagag
     1501 aaggacggat tctttccaga gcaaggattg tgagatagaa gacaaacgga gccaaagtag
     1561 ctgggtagtg cagaccaatt gtatagtgaa gtccatccga cgccaagagc gcagttgcga
     1621 acccgggtgc gagcagaaca aggccgttgc cagcgatcag aggtaagaac agtatcggtc
     1681 cgagcatttg gagcaggaag agaatcttgt tcgcgtcgaa aaaggcaaag taaagagtga
     1741 agaatgggtt cgtgagaagt gtcgcaagca tccccttgag tccggtgaat ccaggggcaa
     1801 tgtagccctc aaatcttgac gccatcggag aaccgtcgtt catgggcatg atgacaccaa
     1861 cgcacagggc aaagtagagt gcacacacaa aacaggtcaa tatgccaact ctgacatttt
     1921 ttctggaaat cgccgcatag acccccagag caaagcaaga cagggccaag tcctccttga
     1981 ccatcaatgt gagaaccagg gaaatccaaa acagcctctt ctgtccggtt tccagcatca
     2041 acagtagaaa caaaagcagc ggggttgcca gaggtatttc atggaaatca cgtatgttgg
     2101 ctccttggat tgccgggtac agcagatagc agatcgacag aaggcatgga atcgtgtgtc
     2161 ctccgagcct tctgcgagcg atcagataca ggggcaccg
//
LOCUS       DAOQUC010000674         2199 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_62279_length_2199_cov_2.080224, whole genome shotgun sequence.
ACCESSION   DAOQUC010000674 DAOQUC010000000
VERSION     DAOQUC010000674.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2199)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2199)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2199
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62279_length_2199_cov_2.080224"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..428)
                     /locus_tag="PKH07_11350"
     CDS             complement(<1..428)
                     /locus_tag="PKH07_11350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35583.1"
                     /translation="MKPRLLLILSIIFATLCGCSEKQDASQRLSADVVKTPIAAPKVV
                     AKFNGGLITDSDLADAEEFWYSMSMDRSSPQTAIPPTDSSDLIRMVAAQSVLAKRARE
                     NGLNESPVFLEMSKRAASRLVVMAYRQKQAQDVSQDEAMA"
     gene            566..1894
                     /locus_tag="PKH07_11355"
     CDS             566..1894
                     /locus_tag="PKH07_11355"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HNT35584.1"
                     /translation="MMKCSIRLLLVSLSILVLCNCGKQAHDTYVWPPPTPSEEHAKVL
                     FIGVDAADWDNIDPMIQQGRIPNIAALKRDSAWGTQKSVSDNSPAIWTSIATGFLPQQ
                     HGIEAFTAENPDYDPNDSDSKRFLSVSSTMRKKKALWNILSEQGRTVGIVGWFATWPA
                     EAVRGYMISSYASLDVNQGGEQLTGKGTFYADRPDLVYPPELGLGDSNTLRLFMEKGK
                     NTALATTERVFAGYPEREGAKAWQINETKWAFAADDIFFQIGKRLLQEYPTDFFAIYL
                     AGLDVIGHRFAGSLEDDEVLRRYYERVDEMIGELISVVPKDTVVVLASDHGTALPGPT
                     HDYEGGIFLYRGRCVESGYCAMQSDSLDFLPTLLYLMGVPVPTQLPKKPMEMLFHDQW
                     RRDNPTRFVEMTLDRKASAPPTGDSYRDEIFKRLSDLGYIGSDGQSKKLE"
     gene            complement(1855..>2199)
                     /locus_tag="PKH07_11360"
     CDS             complement(1855..>2199)
                     /locus_tag="PKH07_11360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35585.1"
                     /translation="PAQNSALAFASSDKAFFAQMGTRVFPERIISLSFHVPKCRHPKR
                     DRETTNATLMNCQTSRSNELLTTQSLPYHGTYEFSVGRTRDIIHVWKEVVNITSNEGD
                     YSSFLDCPSDPI"
BASE COUNT          516 a          526 c          592 g          565 t
ORIGIN      
        1 tacgccatgg cttcatcctg tgaaacatct tgtgcctgtt tctggcgata ggccatcact
       61 accaacctgc tcgccgctct tttggacatt tcaagaaaca caggactctc attgagcccg
      121 ttttctctcg ctcgttttgc aagcacggat tgtgctgcaa ccattcggat caggtcgcta
      181 ctgtctgtgg gcgggatggc agtctgtgga ctcgaacggt ccatactcat tgagtaccag
      241 aactcttccg catccgccaa gtcgctgtcg gtgatcagcc caccattgaa ctttgcgacg
      301 acctttggcg ctgctattgg agtcttgacc acgtctgcgg aaagtctttg cgacgcgtcc
      361 tgtttttcgg agcaaccaca cagtgtggcg aagatgatgc tcagtatcag gagcagcctt
      421 ggtttcattc gttcatacct ccggcatttg cttcttctta cagataccaa tatagcgaac
      481 ttgctccaac aggcaaagtg agccgagtac tacccagaac ttgacttgcc cagaagcctt
      541 gactatgttc gatgcaagga gtgcaatgat gaaatgttcg atacgtctgt tgttggtttc
      601 tctaagcata cttgttctct gtaactgcgg gaaacaggcg cacgatacct acgtctggcc
      661 accgcctacg ccttccgagg agcatgcaaa agtcttgttt attggggtgg atgcggcgga
      721 ctgggacaat atcgatccaa tgatacagca gggcagaatt ccaaatatcg ccgccctcaa
      781 gcgagattcc gcctggggta ctcagaagag cgtctcagac aattctccgg cgatctggac
      841 atcaatagcc acgggttttc tcccccagca acatggtatt gaggcgttta ctgcggagaa
      901 ccccgactac gatccaaatg attcagactc caagcgtttt ctttctgttt ccagcacaat
      961 gcgaaagaag aaagcactgt ggaacatcct gtcggaacag ggcagaacgg tcggtattgt
     1021 cggttggttt gcgacatggc ctgctgaggc cgttcgtggc tacatgatct cgtcgtacgc
     1081 ctcgctggat gtcaaccagg gtggtgagca gttgacaggg aagggaacgt tctatgcaga
     1141 tcgaccagat ctggtctatc cacccgaact gggtttgggt gacagcaaca ccctgcgatt
     1201 gtttatggag aagggcaaga atacagcctt ggcgacaact gagcgggttt ttgctggata
     1261 tcccgaacgc gagggcgcaa aagcatggca gataaacgag acgaaatggg cttttgctgc
     1321 cgatgacatt ttcttccaga ttggcaagag gttgcttcaa gagtatccga cagacttttt
     1381 cgcgatatat ctcgcaggac ttgatgttat tggccatcgg tttgcgggca gtctcgaaga
     1441 tgacgaggtg cttcgccggt actacgagag ggtagatgag atgattgggg aactcatctc
     1501 ggttgttccc aaggatactg ttgtcgtgct ggcatctgac catggaacag ccttgccagg
     1561 tccgacgcac gattacgaag gtggtatttt cctctataga gggcgctgtg tagaaagtgg
     1621 ttactgtgcg atgcagtcgg actcgctgga tttcttgccg acattacttt atctcatggg
     1681 cgttcccgta ccgacccagc tacctaagaa acccatggag atgctttttc atgatcagtg
     1741 gagaagggac aatccaaccc gatttgtgga gatgacactg gatcgaaagg cgtctgctcc
     1801 accaactggc gattcgtata gagatgagat attcaagcgg ctgagtgatc taggctatat
     1861 cggatccgac gggcaatcca agaaactgga gtagtctccc tcattggatg tgatattgac
     1921 aacttccttc cacacgtgaa tgatatccct tgttctgccg acggaaaatt cgtatgttcc
     1981 atgatagggc agactttggg tggtcaggag ttcatttgaa cggctggtct ggcagttcat
     2041 gagcgtagca tttgttgtct ccctatcgcg cttgggatga cggcattttg gaacatggaa
     2101 agagagtgaa ataatgcgtt caggaaagac tcgagtaccc atttgggcaa agaaggcctt
     2161 gtctgaagac gcgaaagcca atgccgagtt ttgtgcagg
//
LOCUS       DAOQUC010000675         2197 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_62345_length_2197_cov_3.163399, whole genome shotgun sequence.
ACCESSION   DAOQUC010000675 DAOQUC010000000
VERSION     DAOQUC010000675.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2197
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62345_length_2197_cov_3.163399"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..612)
                     /locus_tag="PKH07_11365"
     CDS             complement(<1..612)
                     /locus_tag="PKH07_11365"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNT35586.1"
                     /translation="MEKQTVLFGGTIRCAGSPDRVVDAIAVQGDRIVQIGDGAELKSL
                     FPRAHHVSLEGRYLVPGFIDSHCHVELYAHKIQNVNLMGTQSLQEALERIRARVLATP
                     AGKLVIGRGWNKNDWPTHSFPTRLDLDSVSVDHPIVMFSKDGHAAWANSRALLQAGVT
                     RTTADPEGGKIQKDNAGEPSGILFESAEGLLDPIRETPDQKTLQ"
     gene            complement(676..>2197)
                     /locus_tag="PKH07_11370"
     CDS             complement(676..>2197)
                     /locus_tag="PKH07_11370"
                     /inference="COORDINATES: protein motif:HMM:NF013595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HNT35587.1"
                     /translation="VCDVGNNRVLIYKHPIHTDAVADFVLGQDGRFDRAEKGTSPHRL
                     MNPEAAIADASGNILITDTLNHRMLLYAPPESGNIQPIGLWGQWQSLHTGVPDFRGIG
                     PASLEYPVGTAISPDNVLWVADRANHRVLGFEGGTMGDKVADYVIGQVDFVHDAANLL
                     DNTCFFFPRDLAVDRNAHPNRLYVADFENSRVLGFELNKGLKNGAGATLVLGQPDFHS
                     YNPGEGASGMNLPAGVAVDSNGGVYVADRENSRVLYFADPFKTDVTAELVIGQPNFGS
                     FEPNAGRAASAATLNRPEGVSVDGEGNLYVADTRNHRVLRFDRPLETDLIADAVWGQQ
                     GDFSKTEGHSMDKVSAQTFSYPMKLTIGEKGLLAVTDTENHRVIVFDTKAPDPYTPIR
                     ILGQQGRTDTGQDNAGGVSAQSMSGPEGVVFVGHHLYVADTANNRVLFFRDLRDSGDK
                     AERVYGQMGDMTANFIGVARCSARNLWLPSGLDVDSDGSLYVTDREQSRVVVYKAR"
BASE COUNT          490 a          636 c          560 g          511 t
ORIGIN      
        1 ctggagcgtc ttctggtcgg gtgtttcgcg aattggatca agcagacctt cagcggactc
       61 aaaaagtatc ccgctcggct ctcccgcgtt gtctttctgg attttgcccc cttcgggatc
      121 ggccgtggtg cgagtcacgc cagcctgcag caacgctcgg gaatttgccc aggcggcgtg
      181 tccatctttg ctgaacatca cgatggggtg atccacggaa accgaatcca aatccagccg
      241 agtcggaaaa ctgtgcgttg gccaatcatt cttgttccaa ccccgtccga tgaccagctt
      301 tcccgcggga gtcgccagca ccctggcacg gattcgctcc agtgcctctt gaagtgactg
      361 agtccccatg aggtttacgt tctgaatctt gtgcgcatac agttcaacgt gacaatgcga
      421 atcaatgaac ccaggaacga gatatctgcc ttcaagcgac acatggtgcg cccttgggaa
      481 gaggcttttc aactcggctc catcgccgat ctggacgata cggtccccct ggacagcgat
      541 ggcgtccaca actcgatcgg gggagccggc acagcgaatt gtgccaccaa atagaacagt
      601 ctgtttttcc attgttgttt tctccaataa cgggcatcaa agattaagat ctttggactt
      661 aacgaaggca tcatactatc tggccttgta cacgacaacc cgactctgtt ctcgatccgt
      721 aacgtacagg gaaccatccg aatccacgtc cagtccggaa ggcaaccaga gatttcgggc
      781 actgcatcgg gcaactccga tgaaattggc ggtcatgtca cccatctgac catatactct
      841 ttcggctttg tcgcccgaat cgcgaagatc gcggaagaag agaacacgat tgtttgccgt
      901 gtcggcaacg tagaggtgat gcccgacgaa gacaacccct tccggaccgc tcatgctttg
      961 tgcagagaca cctcccgcgt tgtcttgccc tgtgtccgta cgaccttgtt gaccgagtat
     1021 tctgatgggt gtgtacggat cgggtgcttt ggtgtcaaac acaatcactc gatgattctc
     1081 ggtgtctgtc acagccaaca gtcccttttc cccaatcgtc aacttcatcg gataggaaaa
     1141 cgtctgggcg cttaccttat ccatggaatg gccttccgtc ttggagaagt caccctgttg
     1201 accccagacg gcatcggcaa tcagatcggt ttcaagtggt cggtcaaaac gaagaacacg
     1261 gtgattgcgc gtgtctgcca cgtagaggtt tccttctccg tctacgctca ctccctccgg
     1321 tctgtttaga gtggctgccg atgcggctct ccccgcattt ggctcaaacg atccaaaatt
     1381 cggctgtccg atcaccaact cagcagtcac gtctgtcttg aatggatccg caaaataaag
     1441 cactctgctg ttttcccgat cggcaacata gacgcccccg ttggagtcca cagcgacacc
     1501 ggcgggaaga ttcatccccg atgcgccttc tccaggattg tatgaatgga aatcgggttg
     1561 acccagaacg agagtcgcac ccgctccgtt tttcaaccct ttgttcaact caaatccaag
     1621 cacccggctg ttctcaaaat cagccacgta aagcctgttg gggtgtgcat tccgatccac
     1681 agccaaatct cttgggaaga aaaaacacgt gttgtccaac aagttggcgg catcatggac
     1741 aaaatctacc tgtccgatga cgtagtcggc gaccttatcg cccatcgtac caccctcgaa
     1801 tccgagaacg cggtgattgg cgcggtccgc cacccacagg acattgtccg gtgatatggc
     1861 ggttccaaca ggatattcca gggacgcagg cccgattccg cgaaaatcgg gtacccccgt
     1921 gtgaaggctc tgccattgtc cccagagacc tatcggttga atgttcccag actccggcgg
     1981 cgcatagaga agcattcggt gattgagtgt gtcggtaatg aggatgttgc ccgatgcatc
     2041 cgcaattgct gcctcgggat tcatcaaacg gtgcgggctg gtccctttct cagcgcggtc
     2101 aaagcggccg tcctgaccca acacaaagtc agcgacagca tcggtatgga tcggatgctt
     2161 gtagatgagc acccgattgt ttcccacgtc gcaaaca
//
LOCUS       DAOQUC010000676         2196 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_62412_length_2196_cov_2.522186, whole genome shotgun sequence.
ACCESSION   DAOQUC010000676 DAOQUC010000000
VERSION     DAOQUC010000676.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2196
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62412_length_2196_cov_2.522186"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            199..>2196
                     /locus_tag="PKH07_11375"
     CDS             199..>2196
                     /locus_tag="PKH07_11375"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HNT35588.1"
                     /translation="MKTVLLLSVALLVSCPSASHSVSAVPVITNPGFEKFSPDNHAPG
                     WGWYVHSAKASFRSETDKPHSGQRCLVFQNDSTLAAEVYGRLFQHIGVLPSTKYELSV
                     WVRGQNVSRGIHFTDWGCYMLNVPEGTYDWQKISTEFLTGDGQTGLNLGINVTNKCDF
                     LAIDDLSIRPVGRPLAGDGVTGSILLPGQVFGDNQKVYLFVSLDSSWTETGQIRAEIK
                     SGRESLLDVRADIITGQNTFDWEWSSGVGSDREILGHIRIYDSGGRKVTSIKETVEKI
                     SSKVLLKDIGRVEKRVRELDTLLAKCAKQSIPTDYPTVAKTMLEQFIPLAKQDALKGE
                     ERRANYAIKDFKRSLDDAHANLKKYLNEPRLAPIVQRYQTGGVEIDDLSFIGRRKDSR
                     GVLSRGPVFFCGMGHFSQVRSDMPRWPGYGVNIIQVEVGPAITMPSENEIDLKAAQAI
                     AKVLDDAADHNVMVNVLLSPHYFPHWAKEKWPHLGSAGGGFLQYCIYAPEAKRIIESF
                     IRVVVPLLKDKPALHSFCLSNEPMYDGAAECGDTRAAWVEHLTEVHGSVTALNTLYHS
                     EHMSFEDVPIGGDFSAPQFYDYVIFNQRRFAGWHRWMADVIHEIAPDVPVHAKIMWCP
                     ISVRHAVLWGVDPELFGELSQINGNDCMIWPGFVPSSAIASI"
BASE COUNT          490 a          508 c          632 g          566 t
ORIGIN      
        1 gaagtcactg acaatacctt tccttcaggg ccttgtggca tcggatacag caagcggact
       61 gacggatggc cattggatcg tggtgcctac tttgacaact ttgtcgcgga tacgctagag
      121 ccttcgagtc tcaagagtgg ctggttgttg cattagtgtg ggtctctttg gaatatcgtg
      181 atcctcagga ggtcgttcat gaaaacggtt ctgttactat cggtcgccct gcttgtctct
      241 tgtccaagcg catcacattc ggtttctgcg gttccagtca ttaccaatcc aggttttgaa
      301 aagttttctc ccgacaatca tgcacctgga tggggttggt acgtacacag tgcaaaggcg
      361 tcctttcgtt cggagactga caagccgcac tcggggcagc ggtgtctggt tttccagaat
      421 gacagtacat tggccgctga ggtctacggt cgcctgtttc agcatatcgg ggtgctcccc
      481 agcaccaaat atgaactttc agtctgggtt cgtgggcaga atgtctccag gggcattcat
      541 ttcacggatt ggggttgcta tatgctgaat gttccagagg gaacctatga ctggcagaag
      601 atttccacgg agtttctaac cggagatggt cagactggac tgaatctggg tatcaatgtc
      661 accaacaagt gcgatttcct tgcgattgac gatctctcca ttcgtccggt cggaaggcct
      721 ttggcgggag atggagtcac gggctccatt cttcttcctg gtcaggtttt tggagacaat
      781 cagaaagtct atctttttgt gtctctggac tcatcgtgga ctgagacggg gcagattcga
      841 gccgagatca agagtggccg tgagagtctg ttggatgtcc gtgcggacat catcactggg
      901 cagaatacgt tcgattggga atggagcagt ggtgttggaa gcgatcgaga gattctgggt
      961 cacatcagga tttatgattc tggtggcagg aaggttacat ccatcaagga gactgttgaa
     1021 aagatcagtt ccaaagtcct gttgaaggat atcggccgtg ttgagaagcg ggtgcgtgaa
     1081 cttgatacgt tgctggcaaa atgtgccaag cagagcattc cgacggatta ccccacggtt
     1141 gccaagacga tgttggagca gtttatcccg ctggccaagc aagatgcgct caagggggaa
     1201 gagcgacgag ccaactatgc tatcaaagac ttcaagagat cattggacga tgcccacgca
     1261 aatctgaaga agtacctaaa tgagccgagg ctggctccta tcgttcaacg ctatcagaca
     1321 ggcggagttg agattgatga tctgagtttc atcgggcggc ggaaagattc gcgcggagta
     1381 ctaagccgag gaccggtgtt tttctgtggc atgggtcact tcagtcaggt tcgatcggat
     1441 atgcctcggt ggcctggcta tggtgtgaat atcattcagg ttgaagtagg acctgcgatc
     1501 acgatgcctt cggagaacga gatagacctc aaggccgctc aggcaattgc aaaggtcttg
     1561 gacgacgcgg cagatcataa cgtcatggta aacgtgcttc tctcgccgca ctattttccc
     1621 cattgggcaa aagagaaatg gcctcatctt ggttcagcag ggggtggttt tctgcaatac
     1681 tgtatctatg ctcccgaagc gaaacgaatc attgagagct tcattcgcgt cgtagtcccg
     1741 cttctaaagg acaaacccgc attgcacagt ttctgtctct ccaatgagcc gatgtatgac
     1801 ggcgctgctg agtgcggcga tacccgagca gcgtgggtgg agcacctgac cgaagtgcac
     1861 gggagcgtga cagcgctgaa cacactctat cactcggagc acatgtcttt tgaggacgtt
     1921 ccgattggtg gtgatttctc ggcgcctcag ttctacgact acgtgatttt caaccagagg
     1981 cgattcgctg gttggcacag atggatggca gatgtcatcc atgagatagc gcccgatgtt
     2041 cctgttcacg cgaagatcat gtggtgtccg atttcggttc gtcacgctgt gctttggggc
     2101 gtggatccgg aactctttgg ggaactgtct cagataaacg gcaacgactg catgatctgg
     2161 cccggctttg taccatcgtc tgcgatagct tcgatt
//
LOCUS       DAOQUC010000677         2191 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_62635_length_2191_cov_2.710674, whole genome shotgun sequence.
ACCESSION   DAOQUC010000677 DAOQUC010000000
VERSION     DAOQUC010000677.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2191)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2191)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2191
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62635_length_2191_cov_2.710674"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1211)
                     /gene="flgL"
                     /locus_tag="PKH07_11380"
     CDS             complement(<1..1211)
                     /gene="flgL"
                     /locus_tag="PKH07_11380"
                     /inference="COORDINATES: protein motif:HMM:TIGR02550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HNT35589.1"
                     /translation="MVARVSNLMLAERMLRNIQRDLADLAKTHEQFSSGRLFSKPSEN
                     PFGFMTSLRVRELLTSQHRYASTVEGAISNLEFSETLLGSVNGIIQRSQEMAILASNS
                     PMSDTAASSLGEELRQLFEEVFSTANSQNAGRYVFAGTRTDRKPFERGVDELGQAYVV
                     YRGNTDEHRVEVDQGILVTDNMNGLEAFFAKIGTIRAGVPVSDPTTTLLTSVPNVPPP
                     SNGDFSGTFKVNGTTITVTATDTLETLRDKINQANADVVASVDSLNRLVLQSNRTTQV
                     RLEEGTSNVLQALGMLPTIQGTLIGAGLSDTTTLAAMGLTGMEAMRIELGDETLDLDL
                     SSANTLGDVLTLINTSGLGVNAYINANQTGINITSLDSNKDIEISDTRRVFGNSFGIG
                     IDNSMPLFALG"
     gene            complement(1258..>2191)
                     /locus_tag="PKH07_11385"
     CDS             complement(1258..>2191)
                     /locus_tag="PKH07_11385"
                     /inference="COORDINATES: protein motif:HMM:NF018168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar basal body rod C-terminal
                     domain-containing protein"
                     /protein_id="HNT35590.1"
                     /translation="QEPSRILTDSFKTGELGALIELRDSIIPAYQDELDTMVYTLVEQ
                     VNRLHSGGSGLKAFQSLRSAVAVSASTEPLVNAGLAFSAQAGTFTIEIVDSDRNVVES
                     YDITVDPTVDTLADIAARIDAADGTVAGGSLQASVTADNRLQIWTLGGQGFQFHGDDS
                     GVLAALGVNTLFTGSTAEDIGINSIIDQDSDYLATSETGAVGDNGIILKIAQLRDERL
                     MNGGTQTITEFYRATVGQIGIEGRRAEQMRNSSDLLLTALKDQRSSVSGVSIDEELIN
                     LVQAQQAFAAAARVVTMVDEMLDLVTSRLGTGGR"
BASE COUNT          475 a          608 c          584 g          524 t
ORIGIN      
        1 atgccgagtg cgaacagagg catcgaattg tcaatgccga tgccaaatga gttgccgaaa
       61 acccgccttg tatcggagat ctcaatatct ttgtttgagt ccagtgaggt aatgttgatc
      121 cctgtctggt tggcattgat ataggcgttg acaccaaggc cgctcgtgtt gatgagagtc
      181 aagacgtcac ccagtgtgtt tgcggaagac aagtccagat caagtgtctc gtcacccaac
      241 tcaattcgca tggcttccat gccggtcaga cccatcgctg ccagagtggt tgtgtcactc
      301 agacctgctc cgatcagggt gccttggatt gtcgggagca ttcccaatgc ctgcagcaca
      361 ttgctggtgc cctcttccag gcgcacctgt gtggtgcggt tggactgcaa aacgagacgg
      421 ttcagggaat ccaccgacgc gaccacgtcc gcattggcct ggttgatctt gtctcgcagt
      481 gtttccagtg tgtcggttgc cgtgacggtg attgtggttc cattgacctt aaaggttcct
      541 gagaaatctc cgttcgaagg cggcggaaca ttgggcactg acgtcaggag tgttgtggtg
      601 ggatcgctga cagggacacc cgcccgaatg gttccgatct tggcgaagaa ggcctcgagg
      661 ccgttcatgt tgtctgtgac gagaatcccc tgatccactt caacgcgatg ttcatccgtg
      721 ttgccccgat agactacgta tgcctgaccg agttcgtcta cccctctctc aaaaggcttt
      781 ctgtccgtgc gagttcctgc gaagacatag cgccctgcat tctgactgtt ggcggttgag
      841 aagacctcct caaacaactg tcggagttct tcgcccaggg aactggccgc ggtgtctgac
      901 atcggggaat tggatgccag gatggccatc tcctgcgaac gttggatgat gccattgact
      961 gaaccaagca gcgtctcgga gaattccaga ttggaaattg ccccctcaac ggtggatgca
     1021 tagcgatgct gtgatgtcag cagttcccga acccgaagag atgtcatgaa accaaatgga
     1081 ttctcggaag gctttgagaa caatctgcct gaagagaact gctcatgtgt cttggccaga
     1141 tcggcaagat ctctctggat gtttcgcagc atccgttccg cgagcatcag attggaaact
     1201 cgagcaacca ttatcgaatc ctcattttta tctgcgaaga cgatagacca tccgtggcta
     1261 acgtcctccg gttcccagac gggatgtgac caaatcaagc atctcgtcca ccatggtgac
     1321 cacgcgtgcc gcggcggcaa acgcctgctg cgcctgtacc aggttgatca gctcctcatc
     1381 aatcgaaact ccggaaacgc tggagcgttg gtctttgagc gctgtcaaca acaagtctga
     1441 gctgttgcgc atttgttccg cccgccgtcc ctcaataccg atttgaccga ccgtggcacg
     1501 atagaactcg gtgatcgtct gtgtgccacc attcatgagc ctttcatctc ggagctgagc
     1561 gatcttcagg atgattccgt tatccccgac cgctccagtc tcagatgtgg ccagatagtc
     1621 ggaatcctga tcaatgatcg agttaatgcc gatatcctcg gctgtggaac ccgtaaacag
     1681 cgtattgacg ccgagcgcag cgagcacacc gctgtcgtct ccatggaatt gaaagccctg
     1741 tccgcccagt gtccatatct gcagtctgtt atccgccgtg accgatgcct gcaggctgcc
     1801 tcctgcgacg gttccatcgg cggcgtcaat tcgtgccgca atgtcggcca gcgtatccac
     1861 ggtaggatca acggtaatgt cataggattc cacaacattg cggtcggaat ccactatttc
     1921 gatcgtaaag gttcctgcct gcgctgaaaa ggcaagtcca gcattgacca gaggctcagt
     1981 ggacgcactc accgccacag ccgaccgcaa cgactgaaat gccttcagtc cagatccccc
     2041 gctgtggagt cggttgacct gttccacaag agtatagacc atggtatcca actcatcctg
     2101 ataggcgggg atgatcgaat cccgaagttc gattaacgcc cccagttctc ctgtcttaaa
     2161 tgagtccgtg aggattctcg aaggctcctg t
//
LOCUS       DAOQUC010000678         2190 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_62679_length_2190_cov_2.234192, whole genome shotgun sequence.
ACCESSION   DAOQUC010000678 DAOQUC010000000
VERSION     DAOQUC010000678.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62679_length_2190_cov_2.234192"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(109..873)
                     /gene="map"
                     /locus_tag="PKH07_11390"
     CDS             complement(109..873)
                     /gene="map"
                     /locus_tag="PKH07_11390"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003494921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HNT35591.1"
                     /translation="MITLKTPEELQKMREGNRMVTEIMARLLDTVRPGIATSELDQLA
                     EVLIHRRNAKPAFKGYRGYPATICASVNEVVVHGIPNDRVLREGDILSIDLGLSFEGY
                     FADIARTVPVGQVSVQAQRLLEASRKAFFAGFNAAWAHNRVGDISAAVQGSAESMGYS
                     VVRDFVGHGIGMQMHEEPQVPNFGEPKSGARLRVGMTLAIEPMLNIGGFEVQVMDDHW
                     TVVTRDRSLSAHYENTVAVTENGPEILTMLAGGELV"
     gene            complement(910..>2190)
                     /gene="secY"
                     /locus_tag="PKH07_11395"
     CDS             complement(910..>2190)
                     /gene="secY"
                     /locus_tag="PKH07_11395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HNT35592.1"
                     /translation="QLGYHISVPLVNRFELEKLMSRPGGLAGQGTLLGLLDMFSGGAF
                     NKFSIFAMGIMPYISASIILQLLTVVIPFLEELQKKGEEGRKKINQYTRYLTVVITFL
                     QSFMLTYWIVNQGAAMGVRLVENPVLFRPLAAFSMTTGTLVVMWLGERVTERGIGNGI
                     SLIIFANIVARLPDAVGILITRLMLGEGEVGGSIFRDVTWTTTFIIAVLMVAVVASIV
                     VLTQAQRRIPIKRGRQVVGRRTYGGQMSYLPIRINIAGVIPVIFASSLLQFPATIAQF
                     ANSEFLDSMSRMLSPGHWLYEIFYILGIIFFCYFYTAIVFNPKDVAENLQKYGSTVPG
                     FSHGRKTEEYIDQITTRVTLFGAIFLAVIAVMPELVMNYMKIPYAISGFLGGTSMIIV
                     VGVALDTIKAIENHLLLRNYDGFRKKGRMKARSG"
BASE COUNT          534 a          659 c          514 g          483 t
ORIGIN      
        1 aaacatggcg ttgggcagtg gctccactac cgttcctttg acctctataa cattctcttt
       61 cggcatacgt tcctttgaaa ccagactccg aacaagtccg tgcttcgcct aaaccagctc
      121 gcctccggca agcatcgtaa gaatctccgg cccgttctcc gtaaccgcca cggtattctc
      181 gtagtgggcg gacagagacc gatctcgggt caccactgtc cagtggtcat ccatgacctg
      241 aacttcgaac ccaccgatat tcagcattgg ctcgatcgcc aaggtcattc caacccgcaa
      301 cctggcaccg ctcttcggtt caccaaagtt gggtacctga ggctcttcgt gcatctgcat
      361 gccgatacca tgtcctacga agtcacgcac aaccgagtaa cccatggact ccgcgctgcc
      421 ctgcaccgca gcagaaatgt cgcccactct gttgtgcgcc cacgcagcat tgaacccggc
      481 gaaaaaagcc ttccgggaag cctcaagcaa ccgctgcgct tggacactca cctgacccac
      541 ggggacagtt cgagcgatat cagcgaagta tccctcaaaa ctaagcccca agtcaatgct
      601 gagtatatct ccttctctca agacccgatc attcgggatc ccgtgtacca caacctcgtt
      661 cactgaggcg catatcgtcg cgggatagcc tcggtatccc ttgaacgccg gcttggcgtt
      721 gcgcctgtga atcaaaacct ccgccaactg gtccagttca cttgttgcga tccctggccg
      781 aacggtatcc aagagtctcg ccatgatctc cgtcaccatc ctgttgcctt cccgcatttt
      841 ctggagctct tcgggagtct tcaacgtgat catggttcgt tcggtctcca gttgtatacc
      901 tgcctccgat caccccgatc gtgccttcat ccggcctttc ttgcgaaaac catcgtagtt
      961 cctgagaagc aaatggtttt caatcgcctt aatcgtgtcc aaggcaacgc ccacaacgat
     1021 gatcatggat gttccaccca gaaaaccgct gatcgcgtag gggatcttca tgtaattcat
     1081 gaccagttca ggcatcacgg caatgaccgc caggaatatc gctccgaaaa gcgtaactcg
     1141 agtggtaatc tggtcaatat actcttccgt ctttctgcca tggctgaacc caggaaccgt
     1201 tgaaccgtac ttctgaagat tctcggcaac gtccttggga ttgaacacaa tagccgtata
     1261 gaaatagcag aagaagatga tgccgagaat atagaatatc tcgtagagcc aatgtcctgg
     1321 cgaaagcatc cgactcatgc tgtccagaaa ctctgaattg gcaaactgcg caatcgttgc
     1381 cggaaattgg agcagcgacg aggcaaagat gacaggaata acaccggcga tgttgattcg
     1441 aatgggcaga taggacatct gtcccccgta ggtacgtctg cccacaacct gtcgcccacg
     1501 cttgatcggg attcgccgtt gtgcttgggt caacaccaca atcgaagcca ccactgccac
     1561 catgagcacg gcgatgataa aggtcgtcgt ccaagtcaca tctcggaata tggacccacc
     1621 gacctcaccc tctcccagca tcagccgagt gataagaatg ccgactgcgt ctgggagcct
     1681 ggccacaatg ttggcaaaga taatcagcga gataccattt ccgatacccc gttccgtcac
     1741 tcgctcgcca agccacatga cgaccaatgt tccggttgtc atgctgaacg cagccaaggg
     1801 tcgaaacagc accggattct caaccagccg cacccccatg gccgcgcctt ggttcacaat
     1861 ccagtatgtt aacatgaagg attgcaggaa ggtgatgacc accgtcaggt agcgggtgta
     1921 ttggttgatc ttcttacgcc cttcctcacc cttcttctga agctcctcaa gaaaaggaat
     1981 cacgacggtg agaagctgaa gaatgatcga ggcgctgatg tagggcatga tgcccatcgc
     2041 aaagatactg aacttgttaa aggctccccc agaaaacata tccagaagcc ccaggagcgt
     2101 tccctgtcca gcaagacctc cgggccgtga catcaatttt tccagctcaa agcggttcac
     2161 caggggcacg gaaatgtgat agcccagttg
//
LOCUS       DAOQUC010000679         2190 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_62681_length_2190_cov_2.026230, whole genome shotgun sequence.
ACCESSION   DAOQUC010000679 DAOQUC010000000
VERSION     DAOQUC010000679.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62681_length_2190_cov_2.026230"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..694)
                     /locus_tag="PKH07_11400"
     CDS             complement(<1..694)
                     /locus_tag="PKH07_11400"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNT35593.1"
                     /translation="MRNTEHDYRISLNSVLTMLDSTYLSTESLAQPQNCWIRVSTACN
                     VVCPLCPRQHLEPVDSGNMPFDEFQRVASQMVGIERASLLGLGEAMLHRRFFDFVEEC
                     HARGMFVFTSTNGMVLNEEMCRRVVESGIEEFNISMDACTPELFNRLRLGADFEHICT
                     NMKRLSRLRAESGGKLPFLFVSCTVSTENVHEFHTMVPFVHEMGFDSIIFLDFLAAKE
                     HLAHYAVQNTEEF"
     gene            complement(704..1885)
                     /locus_tag="PKH07_11405"
     CDS             complement(704..1885)
                     /locus_tag="PKH07_11405"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNT35594.1"
                     /translation="MDFLRLPERNFRTGRFLSKLPRWLRQRFVRLTQEVLAERMRFAS
                     TPEISRDWNRRLSQFEIRNQTTRVLSRPKRVYFEVTRKCNLRCNMCPHSFKDLDRCDI
                     DLHLLDVIADVLPYNEDMALFGCGESLTTPVFMEALSRLPKDMNTRLITNGLLLDEKV
                     SRLLVQHQLSALTVSVDAAKEDTYQLIHGVSKLQQVIENIETLNRVKKELGSELPALV
                     LGYVLMKANLDELPDFVRLGARLKAASIGVGHLTVFRESLKEQSLLYDREHSNNVLEE
                     TRKLAEILGVHVHLPVPFDLSVDPWKDKFPPFSGLCPEPWEFLYVDNVGTVRPCCIHE
                     EIVGDLHRQSFEEIWNGEPYQRFRAVVNTPKRYGLCLNCFDYRYRNSDNPEHYFLIMA
                     P"
BASE COUNT          553 a          532 c          594 g          511 t
ORIGIN      
        1 tgaactcctc ggtattctga actgcatagt gtgccagatg ctctttcgcg gccagaaagt
       61 ccaggaagat gatcgagtca aaacccatct catgcacgaa aggcaccatt gtatgaaact
      121 cgtgcacatt ctctgtcgaa acagtgcagg agacaaagag aaagggaagt tttccacccg
      181 actcagcacg aagtcttgag aggcgtttca tattcgtaca gatatgttca aagtcagcac
      241 ccagacgcag gcgattgaac aactcggggg tgcaggcatc catggagatg ttgaattcct
      301 cgatgcctga ttcgacaacg cgtcggcaca tttcttcgtt tagaaccatc ccgttggtgc
      361 tggtgaagac aaacatcccg cgggcgtgac attcctcgac aaaatcgaag aaacgcctat
      421 gcagcattgc ttctcccaga cccaacagac ttgccctctc gatgccaacc atctgactgg
      481 cgacgcgctg gaattcatcg aagggcatgt tgccgctgtc caccggctca agatgctgtc
      541 ggggacaaag agggcacacc acattacagg ctgtcgagac acgaatccag cagttctgcg
      601 gttgggcaag actctcggtc gagagataag tcgagtcaag catggtcagg acggagttaa
      661 gcgatattct gtagtcgtgt tcagtgtttc tcatcggatt tccctaggga gccattatca
      721 agaagtagtg ttcgggattg tctgagttgc gatatctgta gtcgaagcag ttcagacaga
      781 gaccgtatcg ctttggagtg ttgaccacag cgcggaagcg ctggtagggt tcaccgttcc
      841 aaatctcttc gaaactctgc ctgtgcaggt cacccacaat ctcttcatgg atacagcaag
      901 gcctgacggt tccgacgttg tctacgtaga gaaactccca aggctcagga cacagaccgc
      961 tgaatggggg gaacttgtcc ttccatggat ccaccgaaag gtcaaaaggt acaggaagat
     1021 ggacgtggac tcccagtatc tcagctaact ttcgggtttc ttcgagaaca ttgttggaat
     1081 gttcgcggtc gtacagcaag gattgctctt tgaggctctc gcgaaaaacg gttagatgtc
     1141 ccaccccaat tgaggccgct ttcagtcggg ctccgagtcg gacgaaatct ggaagttcgt
     1201 ccaaattggc cttcatgagt acgtagccca gcaccagggc aggcagttcg gaacccagtt
     1261 ccttcttgac tcgattgagc gtttcgatgt tctcaatcac ttgctggagt ttactgaccc
     1321 catgtatcag ttggtaggtg tcttccttgg ctgcgtcaac agagacagtc agagcggaaa
     1381 gctggtgttg aaccagaagg cgggagacct tttcgtccag aagaagaccg ttggtaatga
     1441 gccgtgtgtt catatccttt ggaagacgac tcagcgcctc catgaaaacg ggagtggtca
     1501 ggctttcgcc acatccgaaa agcgccatat cctcgttgta tgggagcaca tcggcaatca
     1561 catccagaag atgcagatca atgtcacagc gatccagatc cttgaaagaa tggggacaca
     1621 tgttgcagcg aagattgcac tttcgggtta cctcaaaata tacccgcttg ggacggctca
     1681 ggacgcgcgt tgtctgattg cggatctcga actgtgaaag acgtcggttc caatctcttg
     1741 atatttctgg agttgaagca aatctcatgc gttcagcaag aacttcctgt gtgagtcgga
     1801 caaacctttg ccgaagccat ctgggaagct ttgagaggaa acgtcctgtc cggaaattcc
     1861 gctcaggcag tctgaggaaa tccacgatcc aagacgaaat cctggcagta gaaagtttgt
     1921 cggtagcgcc gctctgtatc aatcaaaagc ctcagtcgga gccttgcctc caagggcaag
     1981 ttgttcactc agtatatagg ggaattcgca aagggtgtaa aggggagttg tgagatttcc
     2041 gcaggacttc ggtgaagtgc ggaaacgcac gcaacctctt gaatacagag ggcaggggga
     2101 ggcgctttag cacatgggga tcaggctgta cctgagcttg accaatcttc ccgtaatcct
     2161 tcagtgaagc gtggagggat ttgggaagag
//
LOCUS       DAOQUC010000680         2189 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_62694_length_2189_cov_3.635426, whole genome shotgun sequence.
ACCESSION   DAOQUC010000680 DAOQUC010000000
VERSION     DAOQUC010000680.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62694_length_2189_cov_3.635426"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..624
                     /locus_tag="PKH07_11410"
     CDS             <1..624
                     /locus_tag="PKH07_11410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35595.1"
                     /translation="DAYPLDGSYPYRCEPLEYDVYNGVTQDLWYRGRVVAKAHPNNTR
                     CSYCCGLTFEIFVRAMQLRNIQKGIDPDDFNGMSFHDLFNLLQLWYIEGPKDSPQAGI
                     EFYGLGQRITDWEQAKPGDFLDYSRTPSSGHSVVFIEWVRDTEGKITGFKYFSSQKNG
                     VGFNTEFFSDTGGKVLRNWMRLARVGSIKDYRPFDRTKIPDRKAYLP"
     gene            780..1862
                     /locus_tag="PKH07_11415"
     CDS             780..1862
                     /locus_tag="PKH07_11415"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNT35596.1"
                     /translation="MKINRREFIKDTVIVSSTVGLTSAFGSSADASFLGLGRKDNSPG
                     NPKKANFRFVHMADQHVFDKRQGTEGYKVCIQSVRALRPKPNFVLMGGDMVFDGCYNT
                     KEDYLRWLKQTREISDQLHCRWYPCMGNHDPFGLSPRRKCDPNDPDIGKQLIMTEFRW
                     PSQKSYYSFDYEGWHFAVLDTTYQVDTESGASQEAKIGSEQMEWLGKDLGQAGDRPKI
                     VVMHVAVFYIKPLADANPEAKAIAAGMMVSDCKDLRLLLERHQVKAVLQGHCHHIEGC
                     LYNGVHYLTSASASGAWWSGDWVGGLPGYTLFHCKGTQMSWKHLSYPWEPRLEDADTL
                     ERERQATYDAEKVEQERQLTKERGLL"
BASE COUNT          537 a          560 c          579 g          513 t
ORIGIN      
        1 gacgcgtatc ctttggatgg cagttatcca tatcgttgtg agccactgga gtacgatgtc
       61 tacaatggcg tgacacagga tctctggtac agaggaaggg tcgtggccaa agcccatccg
      121 aataacaccc gatgcagtta ctgttgcggg ctgaccttcg agatattcgt tcgcgccatg
      181 cagcttcgca acattcagaa agggattgat cctgacgatt ttaacggtat gtcattccat
      241 gatctgttta acctgttgca actttggtat atcgaaggtc ccaaggactc cccccaggca
      301 ggaattgagt tctacggtct aggacaacga atcaccgact gggaacaagc caaaccagga
      361 gatttccttg actacagtcg cacgccatcc tctggccatt ctgtggtgtt catcgaatgg
      421 gttcgagaca ctgagggcaa gataactggc ttcaaatact tctcctcgca aaagaacggt
      481 gtcggcttca acacagagtt tttcagtgac acgggtggca aggttcttcg caactggatg
      541 aggctcgctc gggttggaag cataaaggac taccgtcctt ttgacaggac aaagatccct
      601 gaccggaagg cctatcttcc ctgagccctt tcggcatcgc agttgtgggg cagtgtgatt
      661 gcattgcgtc atggtctggt aggaagccga gttcgtcaga ggtgtttttt cttgcaggca
      721 agtgccgcac aggatttgcg acagtaccgg cgctgaccca ctcaagatga ggttctgaca
      781 tgaagatcaa cagaagagag tttatcaagg acacggtgat cgtcagttcc accgttggcc
      841 ttacttcagc attcggctct tcagcggatg catcctttct cggcctcggt cgcaaagaca
      901 acagcccggg caatccgaaa aaggcaaatt ttcgcttcgt ccatatggca gaccagcacg
      961 tcttcgacaa gcgtcagggc acagaaggct acaaagtctg cattcagtcg gttcgtgccc
     1021 ttaggcccaa acccaacttc gtcctcatgg gcggcgatat ggtctttgac ggctgctaca
     1081 acaccaagga agactatctt cgctggctca agcagacgcg ggagatcagc gaccaactcc
     1141 attgtcgctg gtatccttgc atgggcaatc acgatccgtt tgggttaagt cccagaagaa
     1201 aatgcgatcc gaacgatccc gacatcggaa agcagctcat catgaccgag ttccgttggc
     1261 caagtcaaaa gagctattac agttttgact atgaaggttg gcatttcgcg gttctggaca
     1321 caacctatca ggtggataca gagagcggtg ccagccagga agccaagatt ggctcagagc
     1381 aaatggaatg gctcggcaag gatttgggac aggcgggcga tcgccccaaa atcgttgtta
     1441 tgcacgtcgc ggtcttctac atcaaaccac tcgccgacgc caaccctgaa gcaaaggcta
     1501 ttgcagccgg tatgatggtg tcggactgca aggatcttcg gctgctccta gagcgacatc
     1561 aggtcaaagc ggtcctgcag ggacactgcc atcacatcga aggctgcctt tacaacggcg
     1621 tgcactatct gacctcagca tccgccagcg gcgcatggtg gtcgggagac tgggttggag
     1681 gtcttccagg gtatacactt ttccactgca agggcacgca gatgtcctgg aagcatctca
     1741 gctatccatg ggagccgaga cttgaggatg cggatacact cgaacgcgaa cgtcaggcaa
     1801 cctacgatgc tgagaaggtt gagcaggaac gccaactgac caaggaacgc ggacttcttt
     1861 gagctcactc ctcagggact tcctcgcctc aacactgcat ataccattgc accattgcac
     1921 catttgacag tatctctttg tggtgctaaa gataccaaca taggtatcac tgagcatacg
     1981 cacctgctct gaatcacatt gtcctgatcg cagtgaagcg ttgccaacat ttcgctctgt
     2041 ttggaggtga ccgtaggcgc aataatcatg aagttgcacg aatgaggttt ccgagggact
     2101 aaaagagtat tacttggttc ggagtcaaac atggagggaa tcatgcgaaa gtctttcttc
     2161 gttcttcttg cgcttctggt tgtcagctt
//
LOCUS       DAOQUC010000681         2187 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_62806_length_2187_cov_2.439024, whole genome shotgun sequence.
ACCESSION   DAOQUC010000681 DAOQUC010000000
VERSION     DAOQUC010000681.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62806_length_2187_cov_2.439024"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2187
                     /locus_tag="PKH07_11420"
     CDS             <1..>2187
                     /locus_tag="PKH07_11420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35597.1"
                     /translation="YRTIEIGGDGLCSAESLDPKSVDLLVLPDGSALPADSMKTIENF
                     LRGGGDLIAFRTPLWQRPLICIQGDWLTHEEYALSQAAKVPDPENLLLDFNPSRPVKM
                     FQSSSNKEQQTLCEFAKDEPLKGSHALHVSTPNLTGWDSVVFPDLGKPFPGSHTLTVF
                     SAKGGLKTTQLAVEWTETNGSRWIATIPLTTEWRRYILKPSDFKLWYDFVSTEPYGKV
                     FDPTKAKQISFSLAFTHTTAIGEGAHEYSIAHLGTAAPTGEYQVLAEMTRPPALETLS
                     PTYKFYEMTDVGSVESRSDQSVVSRRSYAVPKSLRSSYARPKGSGFDKDRDWRWIPLL
                     EACSSEGDWRGFPATLLIHAEGVYKGGIWASFSVEERDWYRSSEVLDSVAEIARFMKQ
                     GTYLVDGGSNFYTYFEKQTMTLGVRAVDFESAENLFARVRVFENSMRGSKVLERVWPL
                     SLQSGEIGEVSDVYTPKRWPKKGFRVVAELLHGKQSVDMVSHEAHVWRPKKRKEYITV
                     HNGEFVLKGERWRAHGVNYMPSSGLGTEDGNYFEHYLQKRSYDSEVYSRDLDHVKDMG
                     LNSVSIFIYGDMYTGEADKDQNLLDILRLLEERDLRVNLSLRPGDPVNYYDKHPIDKM
                     LKDFVQYYRLAENDTLFAYDLAWEPSFGHHLERKKWDRDWENWIVERYGSVEAAEQDW
                     GFKVPRDDDGLVTNPLPEHVDSDGDYRVMTAAYRRFLDTLLYKKYRY"
BASE COUNT          513 a          530 c          612 g          532 t
ORIGIN      
        1 taccgaacca ttgagatcgg tggtgatggt ctttgttctg ctgagagcct ggatccgaag
       61 tcagttgatc tcttggtttt gccggacggt tctgctctcc cagcggactc catgaagacc
      121 atcgagaatt tcctgcgcgg tggtggagac ttgatcgcgt ttcggacgcc tctgtggcag
      181 agacctctga tatgtatcca gggcgactgg ctgacacatg aggagtatgc actgtcacag
      241 gcagccaagg tgcctgatcc tgagaatctt ctgctcgact tcaatccttc gcgcccagtg
      301 aagatgtttc agagcagcag caacaaggaa caacaaactc tctgcgaatt tgccaaagat
      361 gaaccactca agggttcaca tgcccttcat gtgtcaacac cgaatcttac aggttgggac
      421 agtgtcgttt tccccgatct cgggaagccc tttcccggat cacacacatt gactgtgttc
      481 tccgccaagg gaggtctcaa aacaacgcaa ctcgcagtgg aatggactga gaccaatggt
      541 tcgagatgga ttgctaccat tcctttgacg actgagtggc gtcgttacat cctcaaaccg
      601 agtgacttca aactctggta tgattttgtc tcaacggaac cttacggaaa ggtcttcgat
      661 ccgactaagg ccaaacagat cagtttcagt cttgcattta cacacacgac agccatcggg
      721 gaaggggcac atgagtattc gattgcccat ctcggaacgg cggctcctac aggtgagtat
      781 caggtgcttg ccgagatgac tcgcccccct gctctggaga cactctctcc cacctacaag
      841 ttctatgaga tgacagacgt aggttcggta gagagtcgtt cggatcagag tgttgtgagc
      901 cgtcgcagtt atgctgtgcc caagagcctt cgttcgtcct atgccaggcc gaaagggagc
      961 gggtttgaca aagatcgcga ctggaggtgg attccgcttc ttgaggcatg ctcttccgaa
     1021 ggagactgga gaggttttcc ggcgactttg ctgattcatg ctgagggtgt ctacaagggt
     1081 ggaatctggg catccttcag cgttgaggag cgagactggt atcgctcaag cgaagttctt
     1141 gattcagtag cagaaatcgc gcgtttcatg aagcagggga cttatcttgt ggacgggggc
     1201 agcaatttct acacctattt cgaaaagcag acgatgacct tgggtgtcag agccgttgat
     1261 tttgagtctg ctgagaacct ctttgcacgc gtgagggttt ttgagaactc catgcggggc
     1321 tcgaaagtgc ttgagagggt ctggcctctc tctctgcagt ccggcgagat tggtgaagtg
     1381 agtgacgttt atacaccgaa gcgctggcca aagaaggggt ttcgagtggt tgctgaactg
     1441 cttcacggaa agcagagcgt tgatatggtg agtcacgaag cgcacgtttg gagaccgaag
     1501 aaacgcaagg aatatattac cgtccacaac ggtgagtttg tcctcaaagg tgaacgttgg
     1561 cgggcacatg gtgtcaacta catgccttct tcgggtttgg gtaccgagga cggcaactac
     1621 ttcgagcatt accttcagaa gcgctcttac gactctgaag tctacagtcg cgatctggat
     1681 cacgtgaagg atatgggtct caactcggtc agcatcttta tctacggcga catgtacaca
     1741 ggtgaagccg acaaggatca gaacctcttg gacatactcc gactgctcga agaaagggat
     1801 ctgcgggtga atctttctct gcgaccggga gatcccgtga actactatga caagcatccc
     1861 attgacaaga tgctcaagga ttttgtccag tactatcggc ttgccgagaa tgacacactc
     1921 tttgcatacg acctggcctg ggaaccttcg tttggccatc atctcgagag gaagaaatgg
     1981 gatcgtgact gggagaactg gatcgttgaa cgctacggaa gtgtcgaggc agcggaacag
     2041 gattggggat tcaaggtgcc tcgtgacgac gatggtctgg tgaccaaccc acttcctgaa
     2101 catgttgact cggatgggga ctaccgagtc atgacggccg cctaccgccg ttttctcgat
     2161 acgcttctgt acaagaagta ccggtat
//
LOCUS       DAOQUC010000682         2186 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_62840_length_2186_cov_3.154388, whole genome shotgun sequence.
ACCESSION   DAOQUC010000682 DAOQUC010000000
VERSION     DAOQUC010000682.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2186
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62840_length_2186_cov_3.154388"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1239)
                     /locus_tag="PKH07_11425"
     CDS             complement(<1..1239)
                     /locus_tag="PKH07_11425"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HNT35598.1"
                     /translation="MRSAEIRKIIAGVEEIPTLPSVATRILTLTSEDSNANGSDLARL
                     IQSDQALASKILKLANSPFYGCRENVSTLQRAISVLGFRMVRNAALSLSVFDLFKSSE
                     KSLSASYEMFNLTDFWRHCAAVALASSLIAKRYGYPQPDEAFMAGLLHDIGKVVLLAH
                     AEEEFRMVLETVRETRGNMLDVEEDLIGIGHTQVGRLLTEKWVLPNSLVDAIWLHHQP
                     VQVSVGSVPNLPTIVRLANSLCHLNHIGASGNGCAYSVYSSVLSSLGIAEKESDKIAK
                     EVLQRLEEFGDLFGLEMCPTEVYLSAVRRANAELGRLNVELDHQNRELEVRRKALDAT
                     KHILRQITPYSLPGETVRIIAASIQNSLETRTAMCFYLDESDEQVYGIVLSQDRPPKE
                     IAWPIRGGVLAPVTSELSSEA"
     gene            1543..>2186
                     /locus_tag="PKH07_11430"
     CDS             1543..>2186
                     /locus_tag="PKH07_11430"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNT35599.1"
                     /translation="MTNPIRILHVRVDGVFGGVERLILDYFRLLNRQKFISILAPMVN
                     RALLAQGAAALGIAVEFVPMRSRFDSSASRRLEEIIRERKIDLVMAYGIRADLTVSQA
                     TQRTGTPWISNVQNLIYNDYSNPVQGRIFYYLDRWILRKADAVTLCARYIKPPLSKGL
                     FKLHNLVHVPNGISFSDPDPKSDSDRLRESIGLSENAQVILCAGRLEHVKGQRLL"
BASE COUNT          535 a          560 c          573 g          518 t
ORIGIN      
        1 cgcctcactg ctcaactcac tggtgaccgg agccaggact ccgccccgaa tcggccaagc
       61 aatctctttc ggtggtcgat cctgagacag gacaattcca tagacctgct catccgattc
      121 atccaggtag aaacacatag cggttcgtgt ttcgagagaa ttctgaatcg aagcggcgat
      181 gattcgcacc gtctctccag gcaggctgta aggtgttatc tgacggagaa tgtgtttggt
      241 cgcatccagt gccttgcgtc gtacttccag ttcgcggttc tggtgatcga gttcgacgtt
      301 cagccgccca agttctgcat tggcgcgtcg aactgccgac agataaactt cggttggaca
      361 catctccaga ccaaaaagat cgccaaactc ttccaaccgt tgaagaactt ccttggctat
      421 tttgtcagat tccttctcag caatgcccag ggaagagagg acagaagagt agacggaata
      481 ggcacatcca tttccactgg caccgatgtg attgagatga cagagactgt ttgccagacg
      541 gacgatggtg ggaagattcg gaacggatcc gacgctgact tgcaccggct gatgatggag
      601 ccagatggca tccacaagac tgttgggaag cacccacttc tcggtcagga gccgaccgac
      661 ttgcgtatga ccgataccga tcaggtcttc ctcaacatca agcatattgc cacgcgtttc
      721 gcgaaccgtc tccaacacca ttcgaaattc ttcctcagca tgcgccagga gcactacttt
      781 tccgatatca tgaagaagac ctgccatgaa ggcttcatct ggctgcggat atccgtagcg
      841 tttggcaatg agactgcttg ccagtgctac cgccgcacaa tgacgccaga aatccgtcag
      901 gttgaacatt tcataggaag cactcaggct cttctcagag gacttgaata ggtcaaagac
      961 cgagagagac agcgcggcgt tgcgaaccat ccgaaagccc agcaccgaga tggctctttg
     1021 aagtgttgac acattttcgc gacagccata gaacggggaa ttggcgagtt tgaggatctt
     1081 tgacgcaagt gcctgatcgg attgaatcag gcgtgccaga tctgatccat tggcgttgga
     1141 gtcctctgac gtgagcgtca gaatacgcgt cgcgacggac ggcaacgtgg ggatctcttc
     1201 tacaccggcg attatttttc ggatttcagc tgatcgcatt atatagaatt ccttaatgtt
     1261 acttcataaa acttaaagct ttttttgagc gaatgtcaag taaaaagtgc ctaaacgggt
     1321 actggctcag ttgtggcggt aatacagcca agatatccta tggaagcaaa gcggagtccc
     1381 ttcggctgcg ctcagggcag gcttcggagc aacgctaccg aagcccgatg cctggatgtt
     1441 tccgcttcgg tagcgtcgca gacgagactg atacgcacac agctcgcttg atcagggttc
     1501 atccagacga tatagtgtct gaaaaccctg gaggcgattg gcatgaccaa cccgatccgg
     1561 attctccacg ttcgggtgga cggagtgttc gggggagtgg aacgactcat cctggactac
     1621 tttcggttgt tgaatcggca gaaattcatc tcgattctgg cacccatggt caacagagct
     1681 cttctcgccc aaggggctgc tgcgcttgga attgcagttg agtttgtccc gatgcgttca
     1741 aggtttgact caagtgcctc aaggcgactg gaagagatca tccgcgagag aaaaatcgat
     1801 ctggtgatgg cttacgggat acgtgcggac ttgaccgttt cgcaagccac gcagagaacc
     1861 ggaactcctt ggatctccaa tgttcagaat ctcatctaca acgattactc gaatccggtt
     1921 caggggcgga tattctacta tcttgaccgt tggattctga gaaaggcgga cgccgtcact
     1981 ctctgtgcca ggtatatcaa acctcctctg agtaaaggac tcttcaaact tcacaatctg
     2041 gtgcacgtcc ccaatggaat cagtttctca gatcctgatc ccaagagcga ctcagatcgt
     2101 cttagagaga gcataggact gtcagagaac gcacaggtca ttctttgcgc cggtcgtttg
     2161 gaacacgtta agggacagag acttct
//
LOCUS       DAOQUC010000683         2185 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_62877_length_2185_cov_3.416432, whole genome shotgun sequence.
ACCESSION   DAOQUC010000683 DAOQUC010000000
VERSION     DAOQUC010000683.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62877_length_2185_cov_3.416432"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2185
                     /locus_tag="PKH07_11435"
     CDS             <1..>2185
                     /locus_tag="PKH07_11435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35600.1"
                     /translation="PGVAGKDDQNTNGDFFVHITKTRMEQEGRSYTLFSGVVGNGLDE
                     ARQSPTQTASEDWSASSLQFCDDETEIFFGFPRQPNQEFWSVTRPWGKSSIQRNIYLP
                     GDGLDNDGDGDIDEPGEEVDDPTESGLGDDQPWQMLDDFREFILSNPAGVSSLLMSGK
                     TLEDVYEALKPIVTVRSRSPIYGPAFFSEDNDTLSRRFLAPNMLNPYTPLKLFLDSVQ
                     DKRQTSIATTLENPDSPQPKLEPLAFPNNIRYLYPLSLDDDRDWTIKQKGKEFFFDLN
                     RNGRPDSDWDGDTETQKPDFWAGAWPLPELARDMDSSLLDDFLDAYEEYEAALYGRYP
                     SLVPKESDRDWDGWMGDHGDFNADGERRYDPEWGVNEDPPGKANGSNDPMPGRDGEDD
                     DNDGGADLQDREVQRARDNAAGDGVDNDGDGMVDEVGETYIVAFDDDEDGKYDEDGPE
                     LQFWANLLDCIDEPPLCDVPEKSEYDGIRSFLASHPYYRPFIYDASVAGGKMTEGMNP
                     TLIDGFTLWTSRMVYDATFFDPTGSSGDSFTEEHPYGKTFFDEPGALTSIRGVEAIRI
                     NEVAAYPIIRLQLEENQGEPTDDWTRKPSANDTEYYEAQRTTTGGDLVHQWDFTHIPP
                     GEYRVVLYQPPGGSDWFDISFGDKIEIQTLDRTADFDFGTGLSAAMKTADAEDQTVEV
                     GSDCTLKVRVTLGEDESTPPEEPDETRVALDYMELIRWRKTKTEPS"
BASE COUNT          527 a          533 c          651 g          474 t
ORIGIN      
        1 ccgggtgtag ctggcaagga tgaccagaac accaacggtg atttctttgt gcatatcacc
       61 aagacccgaa tggagcaaga ggggcgctct tacacgctct tttcaggtgt tgtgggaaac
      121 ggtcttgatg aggcgcgtca gagtccaaca cagacggcaa gtgaagactg gagtgcttct
      181 tcgctccagt tctgtgacga tgaaacggaa atcttctttg gttttcccag gcaaccgaat
      241 caagagttct ggagtgtgac aaggccttgg ggcaaatcgt ccattcagag aaacatctat
      301 cttccaggcg atggtctgga caacgatggt gacggtgata ttgacgagcc gggagaagag
      361 gtggacgacc ccaccgagtc cggtttgggt gacgatcagc cttggcagat gctggacgac
      421 tttcgggagt tcatcctgtc caatccagct ggcgtctcga gtcttctgat gtccggaaag
      481 acccttgagg atgtctatga ggcactgaag ccaattgtta cggtcaggtc gcggtctccg
      541 atctatgggc cggctttctt ctcagaagac aacgatactc tcagtcggcg gtttctcgcg
      601 ccgaatatgc tgaatccata cactccattg aaactgttcc tggattcggt tcaggataag
      661 aggcagactt ccattgccac cacacttgag aatccggact cgccgcagcc gaaacttgag
      721 cctttggcgt ttcccaacaa catccgatac ctgtatccct tgagtctgga tgatgaccgg
      781 gactggacga tcaaacagaa ggggaaggag ttcttttttg acctgaaccg caacggccga
      841 ccggacagcg actgggatgg ggatacggag actcaaaagc cggacttctg ggcgggtgcc
      901 tggccattgc cggaactggc cagagacatg gattcgagtc tgttggatga tttcctggac
      961 gcctatgagg agtatgaggc tgcgctctat ggccgatacc catctttggt tcccaaagaa
     1021 agcgaccgcg actgggacgg atggatgggg gatcatggag acttcaatgc agatggggag
     1081 aggcgttacg atcccgaatg gggagtcaat gaggatcccc cggggaaagc caacggctca
     1141 aatgacccca tgcccggacg tgatggcgaa gacgatgaca atgacggtgg ggcagatctc
     1201 caggatcgag aagttcagcg agccagggac aacgccgccg gtgacggagt agacaatgat
     1261 ggcgacggca tggtggatga ggttggagag acgtacatcg ttgccttcga cgatgatgaa
     1321 gacggcaagt atgatgagga cggtccggaa cttcaattct gggccaacct tctggactgc
     1381 atagatgaac cgcccctgtg cgatgttcct gagaagtccg agtatgacgg catccggagt
     1441 ttcctggcga gtcatcccta ctatcgtccc tttatctatg atgccagcgt cgctggtggc
     1501 aagatgaccg aagggatgaa tccgacgctg atagacggct tcacactctg gacatctcga
     1561 atggtttacg atgccacttt cttcgatccc accggatcat cgggtgactc gtttaccgag
     1621 gaacacccct atggtaagac cttttttgat gaacctggcg ctctgaccag tattcggggc
     1681 gttgaagcca tccgaatcaa cgaagtggct gcctatccga tcatcaggct gcagctggag
     1741 gagaatcagg gcgagccgac ggatgactgg acgcggaaac ccagtgccaa cgatacagaa
     1801 tactatgaag cgcagcgtac aacaacgggc ggcgacttgg ttcatcagtg ggactttact
     1861 catatcccgc ctggagagta cagagtcgta ctctaccagc cacccggcgg gtctgattgg
     1921 tttgatatat ccttcggtga caagattgag attcagacac tggacaggac tgcggacttc
     1981 gactttggca ctggtctttc cgcagcgatg aagactgccg acgctgaaga tcagaccgtg
     2041 gaagttggct cggactgtac gttgaaagtc agggtgacgc tgggggaaga tgagagtacg
     2101 ccgcctgaag aaccagacga aacaagagtg gctttggact atatggagct tattcgttgg
     2161 cgaaagacca agactgagcc gagtc
//
LOCUS       DAOQUC010000684         2185 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_62897_length_2185_cov_2.623005, whole genome shotgun sequence.
ACCESSION   DAOQUC010000684 DAOQUC010000000
VERSION     DAOQUC010000684.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62897_length_2185_cov_2.623005"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..879
                     /gene="rsmH"
                     /locus_tag="PKH07_11440"
     CDS             <1..879
                     /gene="rsmH"
                     /locus_tag="PKH07_11440"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:NF013919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HNT35601.1"
                     /translation="GECFVDCTLGAGAGHSQAIARALGTQGRLVGIDLDSGAIQSARE
                     ILKSFNTPVHLKRGNFADIQTILASVSIHEADGILADLGFSSDQMDQDSRGFSFASEA
                     KLDMRLDPDSQDPSALDLLNTLEESQLADLFWKYGEERLSRRIARAVVREREQGRITT
                     CTELASLVEKVYPRGPHRIHPSTRVFMALRIAVNHELENLERFLPQALSLLRISGRLG
                     VITYHSMEAALVKRFFRRFAGQCICPPGMPVCGCGAIEQGQILTGSKAIVPSASEKDR
                     NPRSRSAQLRVIRKIR"
     gene            1232..1738
                     /locus_tag="PKH07_11445"
     CDS             1232..1738
                     /locus_tag="PKH07_11445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35602.1"
                     /translation="MNMLVSHAFKVSAARTEALRFRSQAWTRPQFWALLTSNILLIWV
                     LGMSSVYLGLKSKQQAYECARLAQTYAELGREIADLQAGIHEMTTVTRIRQVAADNEL
                     GLTPGVQSVILLGPEPSHASMGAVRSRETETRTVSSYLSGRAREVASRVLRVFGEWSL
                     FEQTGGAD"
     gene            1859..>2185
                     /locus_tag="PKH07_11450"
     CDS             1859..>2185
                     /locus_tag="PKH07_11450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35603.1"
                     /translation="MSPVWDGLEKGMNTGNSNRAQIVFVWVMISLCFVAVAARMFQFQ
                     IYLRRVVLNITEEERDKIHVSQITLQPERGRILSRNNTLLAGTLPRDILVMERYNFLN
                     PKNKKEK"
BASE COUNT          495 a          580 c          636 g          474 t
ORIGIN      
        1 ggcgagtgct ttgtggactg cacgctggga gccggagccg gacactcgca ggcgatagcg
       61 agggccttgg gcactcaagg gcggctggtg ggaatagatc tggactcagg cgccattcag
      121 tcggccaggg agattctgaa atccttcaat acacccgtcc atctcaaacg ggggaatttc
      181 gccgacattc agacaattct tgcgagtgtg agcatccacg aagcggacgg gatactggca
      241 gacctgggct tctccagcga ccagatggat caagacagcc ggggcttttc cttcgcgtcg
      301 gaagcgaagt tggacatgcg tctcgatccg gacagccaag accccagcgc cttggacttg
      361 ctgaacactc tcgaagaatc acaactcgcc gacctgttct ggaaatatgg cgaggagaga
      421 ctttcgagac ggatcgcgcg agccgttgta cgcgaaaggg aacagggtcg gattacgacc
      481 tgcaccgagt tggcgtcact agtcgagaag gtctatccca ggggaccgca tcgaattcat
      541 ccaagcacga gggtcttcat ggcgctaaga atcgccgtca accacgaatt ggagaatctg
      601 gaacggtttc tcccacaggc gttgagtctg cttcggattt caggtcgtct gggcgtgatt
      661 acataccatt ccatggaagc agcattggtc aagagattct tccgacgctt cgcgggacag
      721 tgcatctgcc cgccgggaat gccagtctgc ggctgtggag cgatcgaaca ggggcaaatt
      781 ctcacgggat cgaaggcaat tgtgccgagt gcgtcggaga aggacaggaa tcctcgatcg
      841 agatccgccc aactgcgtgt catcaggaaa atccggtgaa tccccatgga gagaacgctc
      901 ttcatgtgga catagaatca gaaaagggtg ttttggtggg ccaggaactg gagcgtcaga
      961 tcctcatgga attgcgcctt gccgtgcgaa aagaagttcc atttccctct aatccaacta
     1021 ccctcctcaa tcagatggag gtgccccccc tccatttgca gagagccccc tattgttcgc
     1081 cgtgggtttg ctccatgagg tgggcacagg cgggagtccg gaagaactct cctcccctcc
     1141 ccagcttatc aaatctggac atgactgcct gtgcgacgat gctgttggtt tctggccccc
     1201 ccttaaacac agttcccagg agcaatgaac gatgaatatg ctggtcagtc atgcgttcaa
     1261 agtgtccgca gcgcgaaccg aagcacttcg attccgcagt caggcatgga caagacccca
     1321 attctgggct ctgctgacaa gcaatatcct cttaatctgg gttctgggca tgagctccgt
     1381 gtacctggga ctgaaatcca agcagcaggc ttacgagtgc gcacgcctgg cgcagacgta
     1441 cgctgagttg ggtcgtgaga tcgccgattt gcaggcaggc attcatgaga tgacaaccgt
     1501 cacccgaatt cgtcaggtgg cggctgacaa tgaactcgga ctgactccag gagttcagtc
     1561 tgtgatcctc ttgggacctg agccttcgca tgccagcatg ggagcggtgc gaagcaggga
     1621 gacggaaacg agaaccgttt cctcgtactt gagtggccgg gcaagggaag tggccagcag
     1681 agtgctcagg gtgtttgggg agtggtctct ctttgagcag acgggtggtg cggactgatt
     1741 gcgttgtgca gactgccgtc tgcggttttc tggtgacatg tcctcggtac ggttgtctgt
     1801 atggacttgt cctttgaaca tgactctccc ctaggtcgtg attgcagctg gcgtcctgat
     1861 gagtccagtc tgggacggac tggaaaaggg catgaatacg gggaatagca acagggctca
     1921 gatcgtcttc gtttgggtga tgatcagcct ttgctttgtc gctgtcgcgg cacgaatgtt
     1981 tcagtttcag atctatctgc gccgcgtcgt gctgaacatc accgaagaag aacgtgacaa
     2041 aatccacgtc agtcagatca ccctgcaacc cgaacggggc agaatcctct cccgcaacaa
     2101 tactttgctt gccggtaccc ttccccgtga catcctcgtc atggaacgct acaacttcct
     2161 gaaccccaag aacaaaaaag agaaa
//
LOCUS       DAOQUC010000685         2182 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_63040_length_2182_cov_2.151387, whole genome shotgun sequence.
ACCESSION   DAOQUC010000685 DAOQUC010000000
VERSION     DAOQUC010000685.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2182
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63040_length_2182_cov_2.151387"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            37..279
                     /locus_tag="PKH07_11455"
     CDS             37..279
                     /locus_tag="PKH07_11455"
                     /inference="COORDINATES: protein motif:HMM:NF024483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HNT35604.1"
                     /translation="MKDLLTYLVKALSERPDEIAIDEKSEGSLVSFTVRVPQPEMGRV
                     IGKAGKTARAIRVLVNTVAYRNNKRANVEFQEEGNA"
     gene            272..835
                     /gene="rimM"
                     /locus_tag="PKH07_11460"
     CDS             272..835
                     /gene="rimM"
                     /locus_tag="PKH07_11460"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HNT35605.1"
                     /translation="MPDRSVAETPATIDLDGWVAFGKIVRAHGIRGEVRIHVFPNQPD
                     FLDSVTEIAVQARSGSTQLCRLESLRQAQPDQWLVRLTGFESREQSQALSGATVYIPE
                     DILPQLEEGEYYTFQLLGLRVESEEGETLGMVVDFESMPANDILIVQTTDTRFPLPVV
                     REVILNVDIAGGRIVVSLPEGLLKLEA"
     gene            835..1527
                     /gene="trmD"
                     /locus_tag="PKH07_11465"
                     /pseudo
     CDS             835..1527
                     /gene="trmD"
                     /locus_tag="PKH07_11465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020814315.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
     gene            1669..1905
                     /locus_tag="PKH07_11470"
     CDS             1669..1905
                     /locus_tag="PKH07_11470"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNT35606.1"
                     /translation="MRLFEVQPGGHTPHHEHEWEHEVYILQGSGAIVSDEGEMEFKAG
                     DAIWVSPKERHQFKNTGAENLRFLCLIPTMGTCT"
BASE COUNT          489 a          553 c          606 g          534 t
ORIGIN      
        1 gcagactcac tccgagcgat ctcgaggagg taatcaatga aggatctgct gacctatctg
       61 gttaaagcac tttccgagcg tcccgacgag atcgccattg acgagaagtc ggaaggctcg
      121 cttgtcagtt tcaccgtacg tgtcccgcag ccggagatgg gaagagtcat cggcaaagcg
      181 ggaaagactg caagggcaat tcgtgtgctg gtcaacacag ttgcctacag gaataacaag
      241 agggcaaacg ttgaattcca agaggaagga aatgcctgat cgttccgttg ctgagactcc
      301 cgcaacgatc gatcttgacg gctgggttgc cttcgggaag atcgttcgtg cccatgggat
      361 tcgaggcgag gtacggatcc atgtttttcc taaccagccg gatttcttgg atagtgttac
      421 tgaaatcgcg gtgcaggctc gttccggttc aactcagctg tgccgtctgg aatcgcttcg
      481 tcaggcacaa cccgatcaat ggcttgtcag gctcaccggt tttgagtcac gagaacagtc
      541 tcaggctttg agcggagcaa cagtctatat tcctgaagat attcttccgc aattggaaga
      601 aggtgagtat tacaccttcc agcttctggg cttgcgagtc gagtcagagg aaggcgagac
      661 gcttggaatg gtggtggatt tcgagagcat gccggcaaac gatatcctga tagttcaaac
      721 gacggatacg cgtttcccgt tgccggtggt tcgtgaggtt attcttaatg tggacattgc
      781 cggcggcaga atagttgttt ctctgccgga gggtctcctt aagctggaag cctgatgcga
      841 attgatatcc tgaccgtctt tccagaaatg tttcaaggcc cgttggattt cagtattgtg
      901 tcacgagcag ttcactcagg gatcgtagaa atccagattc atgatcttcg ggactttacc
      961 gaaagcccac agcgcaagac cgacgatgcg cagtttggcg gtggaagtgg gatgctcatg
     1021 aacgctctcc ccattatcag aggggcagaa gccgttcgag atatggcatt ggagtcttcg
     1081 caggttcgaa tagtgttcct ctccgcttct ggcaaagccc tgacgcagag ccttgctcgc
     1141 gagctttccc tggccgagca acttgtgctg gtctgtggcc attacaaggg cgttgatgaa
     1201 cgcgccatcg agtatttgaa cggtgaagag atttccatcg gagactacgt tctctccggt
     1261 ggagagttac ccgccatggt tcttgtggac gccatcgttc gccttcttcc gggagcggtt
     1321 ggatcgttgg actcggtcga ggaggactca ttctacgatg gcctgctgga ggctccgcgt
     1381 tacaccaggc ctcagaccgt tcgaggcctg agcgttccag aagtcttgct cagcggggat
     1441 catgctgcaa ttgacgcatg gcgtcttgag cagtccttgc agagaacgaa agaacggcga
     1501 cccgatctct acagcaagtg gacgaaggaa catgaatccc atcaggaggc attagagatg
     1561 caaattgtcc attcactcag tgtcaatccc accccagttt cccaggatgc cagcggtgtg
     1621 agcattcgcc cattgctggc tgataacgtg ggtgcacgaa agtttcacat gcgccttttt
     1681 gaggttcaac ctggcggtca cacccctcat catgaacatg aatgggagca cgaggtgtac
     1741 atcctccagg gttctggtgc gatagtttcc gacgagggcg agatggagtt caaggcagga
     1801 gatgctatct gggtctcccc taaggagcgt catcagttca aaaacacagg agctgagaat
     1861 ctccgcttcc tgtgtcttat cccaactatg ggaacatgca cctaggaact gtctagcgcc
     1921 ttcgaacata tcggccacac ctagtattca gtgtgtgccc gtttgagtca cagaaagacc
     1981 cacattgttg ggcagtctct tgggagggtg caattctccg acgcttcttt cctttcatcc
     2041 tggcgtccct tctaattctg gcgatctggg tttcgcggcg tgaaccctat cgcaccacca
     2101 aagccatgca aacttcgacc atggcagtaa atcaagatcc ttccgtcgaa agcatttcac
     2161 cttctgtcgc tgctgctgat cg
//
LOCUS       DAOQUC010000686         2181 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_63063_length_2181_cov_3.685325, whole genome shotgun sequence.
ACCESSION   DAOQUC010000686 DAOQUC010000000
VERSION     DAOQUC010000686.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2181
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63063_length_2181_cov_3.685325"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..338)
                     /locus_tag="PKH07_11475"
     CDS             complement(<1..338)
                     /locus_tag="PKH07_11475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35607.1"
                     /translation="MLSTKFVWGETPHLLKNLTVCLKYERLNIMVSSWIPDEYDGLNL
                     LPGNQTSMRPVSSHEQVFAGVGIAHTPAGAGLLRIDERYDGDSPTDSIDENDPWRPMN
                     VFVHAPGQIG"
     gene            461..2143
                     /locus_tag="PKH07_11480"
     CDS             461..2143
                     /locus_tag="PKH07_11480"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HNT35608.1"
                     /translation="MGQRCSIGLDFGTNSVRALLVDVATGEEIATNVWDYAHGEAGVI
                     VDTKDPNLARQHPADYLKGTEITIREILKQAGDVAPQDIIGIGVDTTGSTPLPVDEKG
                     TPLALHERFTTTPAAMAWLWKDHTGFAEAGEITSLAARIRPHYLAKCGGTYSSEWFWS
                     KMFHCLRTSPEVFEAAYTWVEIADWIPGFLTGTSDPERLKRGICAAGHKAMFNPSWNG
                     YPDEEFLAELDPKLARVRKTLPNRAYTVSDVAGGLSPEWAEKTGLPEGIPVAVGAFDA
                     HLGGVGSGIVPGVLVKIIGTSTCDMMVHPLNRELPDIPGLCGIVPESILPGYYGLEAG
                     QSAVGDIFNWFVNSIQPGGAQKGSHEELTRQAEKLRPGESGLLALDWHNGNRTILVDQ
                     RLTGMILGMTLHTTPAEIYRALIEATAMGARTIAERFEEYGVKVERVVNCGGISAKNP
                     LAMQIYADVMGRPLDISRSGQTCALGAAMAGAVVARRAKGGFDRFEDATPVMTGVLDR
                     KFNPNPDSCRIYDRLYVHYRRLHDSFGTTQSQENLFSVMKELLVIRDEVRKQ"
BASE COUNT          506 a          564 c          618 g          493 t
ORIGIN      
        1 tgaccgattt gcccaggcgc atgcacaaaa acattcatcg gacgccacgg gtcattctca
       61 tcaatgctgt cggtgggtga atccccgtca taccgctcgt caattctcag caggccagcg
      121 cctgcaggcg tgtgggctat gcccactccc gcaaacactt gctcatggga agaaacaggc
      181 ctcatggaag tctggtttcc cggaagcaga ttcagaccgt cgtattcatc tggaatccat
      241 gaactcacca taatgttaag tctctcatat ttgaggcaca cggtcaaatt cttcaggagg
      301 tggggcgtct cgccccacac aaatttcgtc gagagcaaga tgccccactc ctttgcgatt
      361 caactgctgc atgagtgctg ccctacggct gaaatttgga aatcgccagc gacttgtggt
      421 accttaagtc ttcaaacact cagccgtcag gaggagagag atgggtcaaa gatgttcaat
      481 cggattggat tttggaacga attccgttcg agcgttgctg gttgacgttg ccaccggtga
      541 ggagattgcc acaaatgttt gggattatgc tcacggtgag gcgggtgtca tcgttgacac
      601 caaagatccg aatctcgccc gacagcatcc tgcagactat ctgaagggaa ctgagatcac
      661 catcagagag atattgaagc aggccggaga cgtggcaccc caagacatca tcggcattgg
      721 tgtggacaca accggaagca cgcctttgcc cgtggacgag aaagggactc cactggcact
      781 gcatgaacgc ttcacgacca ctcccgctgc catggcatgg ctgtggaaag atcacacagg
      841 ttttgccgaa gcaggagaga tcacatcttt ggccgcgagg attagacctc attacctggc
      901 taagtgtggc ggaacctatt cgtcggaatg gttttggtca aagatgtttc actgcctgcg
      961 aacatcaccg gaagtctttg aggctgccta cacgtgggta gagattgccg actggattcc
     1021 cggatttctg acgggcacct cagatcccga gcgactcaag agaggcatct gtgccgctgg
     1081 ccataaggca atgttcaatc cttcatggaa cggctatccg gacgaagagt ttcttgctga
     1141 attagatccg aagctggcgc gagttcgaaa gacacttccc aaccgggcgt acaccgtttc
     1201 ggatgtcgct ggcgggctct caccggaatg ggctgagaag actgggttgc cggaaggaat
     1261 ccccgttgcg gtcggagcct ttgatgcaca tctaggcggt gtaggttctg gcatcgtacc
     1321 gggcgttctg gtgaagatca tcggcacttc aacgtgtgac atgatggtgc atcccctcaa
     1381 cagggagttg cctgatattc caggactgtg cggaattgtt cctgaatcca tcctgccggg
     1441 ctattacggt ctggaggctg gacagtccgc agtgggagac attttcaact ggtttgtgaa
     1501 ctccatccaa cccgggggcg ctcagaaagg atcccacgag gaattgacac gccaggcgga
     1561 gaaactccgc cccggcgaga gcgggctttt ggctctcgac tggcacaatg gaaaccgaac
     1621 cattcttgtt gaccaacgtc tgacgggcat gattctgggt atgacgctcc ataccacacc
     1681 ggccgagatt taccgtgcgc tgatcgaagc cactgcaatg ggtgctcgaa ccatcgcgga
     1741 acgttttgag gaatatggcg tgaaggtgga acgtgtcgtg aactgtggag gcatctccgc
     1801 caagaatcct ttggcgatgc agatttatgc tgatgtgatg ggacgcccgt tggatatctc
     1861 acgcagcgga cagacctgtg cgcttggtgc agccatggcg ggcgctgtgg tggcgagaag
     1921 agctaagggt ggtttcgacc gcttcgaaga cgccacacct gtcatgactg gggtgctgga
     1981 tcggaagttc aatccgaacc ccgacagttg tcgcatctac gatcgcctct acgttcacta
     2041 tcgccgactg catgacagtt ttggcacaac acagtcccaa gagaatctct tttccgtcat
     2101 gaaggaactc ttggtcatcc gagacgaggt tagaaaacaa tgagttttca ggcattgaag
     2161 gagtcggtct gccatgcaaa t
//
LOCUS       DAOQUC010000687         2179 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_63152_length_2179_cov_3.500942, whole genome shotgun sequence.
ACCESSION   DAOQUC010000687 DAOQUC010000000
VERSION     DAOQUC010000687.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2179
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63152_length_2179_cov_3.500942"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..844
                     /locus_tag="PKH07_11485"
     CDS             <1..844
                     /locus_tag="PKH07_11485"
                     /inference="COORDINATES: protein motif:HMM:NF015894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HNT35609.1"
                     /translation="GEKLSVDPVYVIQGSVDLGTGNVSHKGAVVISEDITAGFRIEAL
                     GDIEVAGVVEPSWISAEGNLVVRGGITGSPENRIEVKGNLSARFIQDAIIEMQGDIIV
                     ESEIMHSTIRTLGKLSIPRGRFVGGSIIARGGIELKEAGGAGATPTLLAAGEDYLLQQ
                     QLEPRQANLKRFEANLEKMKIAVEPMMSRIKLLNSKQREALTALMAKITEIDMEVTTL
                     QQEIADLENESREQSVREIHISGVVYPETTLRIDGCGFRVLKTTMGPLRAILVARKIV
                     LEHR"
     gene            1027..1518
                     /locus_tag="PKH07_11490"
     CDS             1027..1518
                     /locus_tag="PKH07_11490"
                     /inference="COORDINATES: protein motif:HMM:NF025070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HNT35610.1"
                     /translation="MKADYINPFIESVHEIFTTMLGCNLERGDVSLSKAGGGTRDLTA
                     LIGLSGPARGMVALSFPVKTALSVVGALLGSEIRIVDDTVSDGVAELVNMVAGAAKAK
                     FCRGDSIPIDLSLPTVVRGNSYSVDYPSHSTWLEIPFKCDLGSFDLRVTFEMDSSRNG
                     GAK"
     gene            1515..1874
                     /locus_tag="PKH07_11495"
     CDS             1515..1874
                     /locus_tag="PKH07_11495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNT35611.1"
                     /translation="MKALVVDDSAVMRKIMIAAIEKAGVTDVVQAGDGQEAVDAVSGN
                     EFDVVLMDWNMPNMLGIDAVKAIRALGKKMPIIMVTTEAEKTRVIEALKSGATSYVIK
                     PFKPESIVEKISEVLKK"
BASE COUNT          523 a          468 c          668 g          520 t
ORIGIN      
        1 tggggagaaa ctgtcggttg atccggttta cgttatccag ggaagtgtgg atcttggaac
       61 tgggaacgta tctcacaagg gtgcagttgt catcagtgaa gacatcaccg cgggatttcg
      121 tattgaagcg cttggcgaca ttgaagtggc tggtgtggtt gaaccttcct ggatctcagc
      181 ggaagggaat cttgttgttc gagggggcat cacgggatca ccagaaaacc gcattgaggt
      241 caaggggaat ctctctgccc gattcattca agacgccatc atcgagatgc agggcgacat
      301 cattgttgag agtgagataa tgcactccac gatccgtacc cttgggaaat tgtcaatccc
      361 gagggggaga ttcgttggtg gaagcatcat tgcccgaggt gggatcgaac tcaaggaagc
      421 gggtggggct ggagcgacgc ccactcttct tgctgcaggt gaggattatc ttttgcagca
      481 gcagttggaa cccaggcagg caaatctcaa gcgctttgag gccaaccttg agaagatgaa
      541 gattgctgtc gaacccatga tgagtcggat caaactgctc aactccaaac agagagaggc
      601 gttgactgct ctgatggcaa agataaccga gattgacatg gaagtgacga ctcttcagca
      661 ggagatagcg gatttggaga atgagtccag ggagcagtct gtcagggaga tccatatctc
      721 gggagtcgtc tatccggaaa cgacgctccg aatagacgga tgcggtttcc gagttctgaa
      781 gacaacaatg gggcccttgc gcgcgattct tgttgcgcga aagattgtct tggagcatcg
      841 ctagtctttt gctctggcca ctggcccagg atgtgcattt gctccaacgg aaggcaggca
      901 ttcttggtca agtgcagttc tgcaaagagg gtgcttttgg ggagagattc tggtttacta
      961 tgagtgtgtc tggtagagtg cttgtctctc tgctgcggac gaacaccagg aatatggagg
     1021 taaggaatga aggccgatta catcaaccca ttcattgagt cagtgcatga gatattcacg
     1081 accatgttgg gctgcaatct ggagcgcgga gatgtctcgc tgtcgaaagc tggaggaggg
     1141 actcgagatt tgacggcgct gattggactc agtgggcctg ctcgtggcat ggtcgcgttg
     1201 agctttcctg tcaagacagc cctgagtgtc gtcggagcgc ttctcggatc cgagattcgc
     1261 attgtggacg ataccgtttc agacggtgtt gcagaattgg tcaacatggt ggctggcgcc
     1321 gcgaaagcga aattctgtcg aggggactcc atcccaattg acttgagtct tccaaccgtt
     1381 gtacgcggta acagctacag tgtggactat ccatcccaca gcacatggtt ggagattccc
     1441 ttcaagtgtg acctcggttc ctttgacctt cgggtgacat ttgagatgga ttcgagtcgt
     1501 aatggaggtg ccaaatgaaa gccttggttg ttgacgattc ggctgtaatg cgaaaaatca
     1561 tgattgcggc gattgagaag gctggggtga ctgatgtcgt ccaggcaggt gacggtcaag
     1621 aggcggtgga tgcagtcagc ggcaacgaat ttgacgtcgt cttgatggac tggaacatgc
     1681 caaacatgtt gggtattgat gcggtcaagg ccattcgcgc tttgggtaag aagatgccga
     1741 tcatcatggt tacgacagag gcggagaaga ccagggtcat cgaggcactg aagtccggtg
     1801 cgacgagcta tgtcataaag ccgttcaaac cggaatccat tgttgagaag atcagtgaag
     1861 tgctcaagaa gtgaggcgga ctcaaggtcc gaacatggaa cgcagaacga gaagaaatac
     1921 gagtctttcg acggaatctg gatattttct ggaaccaggt ttcatctact tcggtcgcca
     1981 gccgacgacg attcgaaccg tgctcggaag ctgtgttgcg gtttgtttgt gggacccggt
     2041 tcaagggata ggtggaatga atcactatct ttatccattt acccgggata gacggacggc
     2101 aacaccaaag tttggcaacg tcgccatcgc tgcccttgtc cgattgatgg acgcagcggg
     2161 aagtgtacgt aaaaacgtg
//
LOCUS       DAOQUC010000688         2175 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_63346_length_2175_cov_3.405189, whole genome shotgun sequence.
ACCESSION   DAOQUC010000688 DAOQUC010000000
VERSION     DAOQUC010000688.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63346_length_2175_cov_3.405189"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..646)
                     /gene="jag"
                     /locus_tag="PKH07_11500"
     CDS             complement(<1..646)
                     /gene="jag"
                     /locus_tag="PKH07_11500"
                     /inference="COORDINATES: protein motif:HMM:NF041568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HNT35612.1"
                     /translation="MKKQIESTGKSVEEAIRNGLAELMASRDDVEVKVLQVETPRLFG
                     LLGKTQARVRLTCLESDDKATAVRLTREIIGLMGMQAEVSARDENGQILVEIRGGGDL
                     LTGQQGRPLDALQYLVSRVVNTGRKDEEWTRINIDLEGYRDKRTDALEGLARKLARQA
                     VEQGREIITDPMPAHDRRVIHLTLKPEKDVETFSRGQGEMRRVVISPKEKRRYSA"
     gene            complement(667..>2175)
                     /locus_tag="PKH07_11505"
     CDS             complement(667..>2175)
                     /locus_tag="PKH07_11505"
                     /inference="COORDINATES: protein motif:HMM:NF026199.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family insertase periplasmic-domain
                     containing protein"
                     /protein_id="HNT35613.1"
                     /translation="EEMTLEEAQTKLAPEMLPYFRAYLDLKSKFEQARQFEDAQKNLR
                     SPFPDDVAVELIPQEGPQPIGTFLLECNGNALDSEMVYACESSEIIVETTTTVTFSVR
                     TDELEIQKVYTFYPDRYEGRLDIHATYLTGEETADRGTLELVATRALGRYLEGASKGR
                     KGVAGQAAIVQLNGKMKKMPQQGQKLGTVREYVGTVNWFGMENRYFIAALVPHNELEL
                     ARTRTRDVQGTSDVSVALPIGRIAPGTPKHFGFHMFMGPKDTDLLAPLGAGVHNAVFS
                     TGFLRALRLSWICPLILWLLKALYRVIPNYGVGILILSVLAKWVTYPLTIKQLRAMKK
                     MQELSPQIQELREKYKDNPRELQKKTMELYSQHGANPMGSCLPMLLQMPVFIGLFMTL
                     NYSIQLRGAPFALWIDDLSKPDALFYIPFSLPMFGFVSLNVIPLVMGLITYLQQSKQV
                     VDPQQAFMTKFMPLFFMVLLWNFPSGVILYWTVSSLLQGVQQRVMDQPKHGG"
BASE COUNT          479 a          609 c          550 g          537 t
ORIGIN      
        1 gagccgaata ccttcgcttt tccttgggag agatcacgac acgtcgcatc tcaccttggc
       61 cgcggctgaa tgtctccacg tccttttcgg gtttcagtgt cagatggatg acgcggcggt
      121 catgcgccgg catcggatcg gtgatgatct cccgaccttg ttccacggct tgtctggcaa
      181 gttttcgggc aagaccttcc agggcgtcgg tgcgtttgtc tcgatatcct tcaagatcaa
      241 tgttgatccg agtccactcc tcgtcttttc gaccggtatt gaccacgcgg gagaccagat
      301 actggagcgc atccagaggt ctgccctgtt gtcccgtgag aaggtctcca ccgcctcgga
      361 tttcaacgag aatctgccca ttctcgtcac gggcagatac ctctgcctgc atccccatca
      421 gaccgatgat ctctcgggtc aggcgaaccg cggtggcttt gtcatcactt tcaaggcagg
      481 tcaaccggac acgtgcctgt gtctttccca gcagtccaaa aagtcgcggt gtttccacct
      541 ggagcacttt cacttccacg tcatcgcggg aagccatcag ttctgccagt ccatttcgga
      601 tggcctcttc aacactcttg cctgtgcttt caatttgctt cttcatggct caagaaatcc
      661 ctctctctaa cccccatgct tgggttgatc catcactctt tgttgtactc cctgaagcag
      721 tgaactgacc gtccagtata gaattacgcc cgacgggaaa ttccacaaca acaccatgaa
      781 aaagagaggc atgaacttcg tcatgaatgc ctgttgtgga tccaccacct gcttgctctg
      841 ttgcagatac gtaatgagtc ccatcaccaa ggggatcaca ttcagtgaaa caaaaccgaa
      901 catcggcaga ctgaaaggga tgtagaacag agcatccggt tttgaaaggt cgtcaatcca
      961 cagcgcaaaa ggcgctcccc tgagctgaat ggaatagttc agtgtcatga aaagaccgat
     1021 gaacacgggc atctgaagca gcatgggaag acacgatccc atcggattgg ctccgtgctg
     1081 gctatacagt tccatggtct tcttctgcag ttcccttgga ttgtccttgt acttctcgcg
     1141 taattcctga atctgcggag acagctcctg catcttcttc atggcgcgca gctgcttaat
     1201 cgtcaaagga taggtcaccc acttggcgag aacggagaga atcagaattc cgacaccgta
     1261 gttggggatg acccgataga gcgctttgag cagccagaga atcagcgggc aaatccagct
     1321 gagacgcagc gcccgcaaga atcctgtcga gaacaccgca ttgtgaactc cagcgccaag
     1381 aggagccaac aaatcggtgt cctttggccc catgaacata tggaatccga aatgcttcgg
     1441 ggttcctggg gcaattcgtc cgatgggcag agcaacgctg acgtccgaag ttccctgcac
     1501 atcacgtgtt cgcgttcgcg ccagttccaa ctcattgtgg ggaaccaggg cagcgatgaa
     1561 atagcgattc tccatgccga accagtttac cgtgccgaca tattcacgaa cagttcccaa
     1621 tttctggcct tgctggggca tcttcttcat tttaccgttc aattgaacga tggcggcttg
     1681 tccggccaca ccttttcgtc ccttggaagc gccttccaga tagcgtccca atgcccgggt
     1741 tgcgaccagt tccagcgttc ctcgatcggc cgtttcctcg ccggtcagat aggtggcatg
     1801 aatgtccagc cgaccttcat atcggtcggg atagaatgtg tagactttct gaatttccag
     1861 ctcgtcggtg cgaacagaaa aagtcacagt tgtcgtggtt tcgacgatga tttcggagga
     1921 ctcgcaagca tagaccatct ccgaatccag agcgtttccg ttgcactcca gcaggaaagt
     1981 cccgatgggt tggggccctt cctgcggaat cagttcgacc gccacgtcgt caggaaatgg
     2041 tgatcggaga ttcttctggg cgtcttcgaa ctgcctggcc tgttcaaact tgctcttcag
     2101 atcaagatag gctcgaaagt agggcagcat ttccggagca agtttggtct gtgcttcttc
     2161 taatgtcatc tcttc
//
LOCUS       DAOQUC010000689         2174 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_63424_length_2174_cov_2.530911, whole genome shotgun sequence.
ACCESSION   DAOQUC010000689 DAOQUC010000000
VERSION     DAOQUC010000689.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2174
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63424_length_2174_cov_2.530911"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2174)
                     /locus_tag="PKH07_11510"
     CDS             complement(<1..>2174)
                     /locus_tag="PKH07_11510"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNT35614.1"
                     /translation="TERLVPVAGAEVALHRINSATQVFDRPLRTRTDDRGHYLVENVP
                     QGLYLIQARKRGVGMAQARLNIEAEIVARQDLILENPISLGGKLTGTVVEDTGNLDLW
                     IPIPGARVTVMLPDGKSKTALTNRIGYYEIGDVQPGFHRVIAEKAEFEPQRQTVHVRP
                     FQETEANFRLRKIAEKVGALRGTVCTQQQGIGEAPTIQVPLPGSLVTLFPAGRCPDKA
                     FEAEENIPISRFSAWTNEEGQYAIRSIPAGEYVAIAFHRGYLPQRIVVTIVAETVTEQ
                     DFTLLPKPAPQMGSVAGMVIEDVGALDVLIPISEATVAMLNLDGRERFVTRSDEQGQF
                     AFPEVPAGRYIGRACKEGYVPKSQPISVLPNEETRTTFALKKDLPIRTGEVFGSVVVI
                     SETAAGVTEQEPIADARVILLPCGIPWMRHRDRAVRPGIPPLETQTDASGDYRFVEVQ
                     PGSYLVCVEAEGYPRATKPATVLPDEQVEVNFAFPIQQTPTFGTLVGVVTDQSTGDPI
                     IGAELVLWRQNFGAFARTISGEEGRFEFSRITAGEYILKAYMRGYVGFHTPVTVLANE
                     TVTQSVELVPVTNPPTATPRPEPTQTPKPEPTRTPSPTPTSTPRPELGSISGVVQTGS
                     SSGALVPVPDAVVKLIRPDTSEWIRTQRTDSRGRYWFENVPGGVYVLKVLKEGFAVAE
                     RQVFLATGQNLEANFVLQPIVRLGAIYGVVFEELNSGSSVTH"
BASE COUNT          460 a          601 c          598 g          515 t
ORIGIN      
        1 gtgtgtgaca gaggagccgg aattcaactc ttcgaagacc actccgtaga tcgcgcccag
       61 tctgacaatc ggctgcaaca cgaagttggc ttccaggttc tgtcccgtcg caaggaatac
      121 ttgcctttca gccacggcaa aaccttcctt gagcactttg agaacataga ctccaccggg
      181 aacgttctcg aaccagtaac gccctcttga gtctgtcctt tgcgtacgaa tccactccga
      241 ggtgtcagga cggatgagct tgaccacggc atccggcaca ggcaccaacg ctccgctgga
      301 actgccagtc tgaaccacgc cagatatgct ccccagttca ggtctcggcg tggatgttgg
      361 agtcggtgac ggagtcctgg tcggctccgg cttcggtgtc tgagttggtt caggtcgagg
      421 cgtcgctgta ggcggattgg tcactggaac gagttcaaca gactgagtga cggtctcatt
      481 tgccagtaca gtgaccggag tgtggaagcc aacatagcct cgcatatacg ccttgagaat
      541 gtactcacca gcagtgattc gagagaactc aaaacgtccc tcttcgcctg agatcgttct
      601 ggcaaaggcg ccgaaattct gtctccagag aaccagctcg gcaccgataa tcgggtcgcc
      661 agtactttga tcagtgacta caccaaccaa ggtgccaaag gttggagtct gctgaatagg
      721 aaaggcgaag ttcacttcaa cctgctcatc aggtagtact gtggcaggct tggtggctct
      781 gggatagcct tctgcttcca cacagaccag ataactgccg ggctgaactt cgacgaagcg
      841 gtaatcaccc gaggcgtcag tctgagtctc cagcggcggg atacccggtc tgaccgctcg
      901 atctcggtgt ctcatccagg gaattccgca cggaaggaga ataactcgag catctgctat
      961 gggttcctgt tcagtgacgc cagcggccgt ttcactaatc accaccacac tgccgaagac
     1021 ttcgccggtt cggatgggaa ggtctttctt gagtgcgaag gtcgtcctgg tctcctcatt
     1081 cgggagaaca gaaatgggct gactcttggg aacataacct tctttgcatg cccttccgat
     1141 gtatctgcct gcgggaactt cagggaatgc aaactgtccc tgttcgtcgc ttcgagtgac
     1201 gaagcgctcg cgaccatcca gattcagcat agccacagtg gcctctgaga tgggtatcag
     1261 aacatcgagt gcgccaacgt cctcgataac catgcctgcg acgcttccca tctggggagc
     1321 cggtttgggt agaagagtga agtcctgttc ggtcaccgtt tctgccacga tcgtgaccac
     1381 aattctctgg gggaggtagc ctctatggaa ggcgatggca acgtattcac ccgcaggaat
     1441 gcttcggatt gcgtactgcc cctcctcgtt tgtccaggcg ctgaagcggg aaatcgggat
     1501 attctcttcg gcttcaaaag ctttgtcagg acaacgtccc gcggggaaga gagtcaccag
     1561 ggaaccggga agcggaacct gaatggttgg cgcttcgcca atgccctgct gttgggtgca
     1621 aactgttcct cgcagcgctc caaccttctc tgcgatcttg cggagtctga aattggcttc
     1681 ggtctcctgg aaaggtcgta catgaactgt ttggcgttgt ggttcgaact ctgctttttc
     1741 cgcaatcact cgatggaatc cgggttgaac atcgccaatc tcgtagtaac cgattcgatt
     1801 tgtgagcgcc gtcttggact tgccatcggg cagcatcaca gtcactcggg cacccgggat
     1861 cggaatccag agatccagat tgcctgtgtc ctcaaccact gtgcccgtca gttttccgcc
     1921 cagactgatc gggttctcca agatgagatc ttgtctggcg actatttcag cctcgatgtt
     1981 cagtcgagcc tgagccattc caacgccacg cttgcgcgcc tgaatcaggt agagtccttg
     2041 aggcacgttt tcaacgaggt aatgacctcg gtcatccgtt cttgttctta gtggtctgtc
     2101 gaagacttgt gtcgcactgt tgattcgatg cagagccact tcggcacctg caacgggaac
     2161 aagtcgttcc gtgg
//
LOCUS       DAOQUC010000690         2172 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_63521_length_2172_cov_2.641001, whole genome shotgun sequence.
ACCESSION   DAOQUC010000690 DAOQUC010000000
VERSION     DAOQUC010000690.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63521_length_2172_cov_2.641001"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(122..568)
                     /locus_tag="PKH07_11515"
     CDS             complement(122..568)
                     /locus_tag="PKH07_11515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35615.1"
                     /translation="MCLFIVLVLLGCGPAEPPEQNVSEVKVEPFLPPTDEQVRAIITE
                     MGGLAIGVTNPNLFVVNQTRLIAYKVDPEGSCRVRVQVEGHLTEPLVTLNAVYDVSQT
                     LSYESEYRLRRSEEGWKYATLVEANMLAFPESDLQLSSDEQRSATE"
     gene            complement(782..967)
                     /gene="rpmB"
                     /locus_tag="PKH07_11520"
     CDS             complement(782..967)
                     /gene="rpmB"
                     /locus_tag="PKH07_11520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HNT35616.1"
                     /translation="MARACQVCGKGAQFGHNVSHANNRTKRRFFPNLHTIRVAQGGGN
                     RRKMRVCMDCINKIRTK"
     gene            complement(1102..1176)
                     /locus_tag="PKH07_11525"
     tRNA            complement(1102..1176)
                     /locus_tag="PKH07_11525"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1142..1144),aa:Gly,seq:gcc)
     gene            1546..2028
                     /locus_tag="PKH07_11530"
     CDS             1546..2028
                     /locus_tag="PKH07_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012530386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNT35617.1"
                     /translation="MSPTRTIGRRTPSRIGHQPFSILLVEDNLDHAELIRRGFGAHHI
                     EDAIVHVRDGEEALDYLLCHGKYAQRKDRDNPHLILLDLRLPKIDGLEVLKVIKNTEG
                     ICRIPVVILTTSDAERDVSQAYDRRANSYVIKPLDFQTFIQLTKDLGLYWLGWNLHPC
                     "
BASE COUNT          530 a          535 c          577 g          530 t
ORIGIN      
        1 aagaatctga cagaaatcgg ccggagcccc gccaaccgaa acatgaggga tttcaatcgc
       61 tctcctgtcg cgagactaat gatttgatat aaaaggagtt aaatgtggca gtgcactatc
      121 tctactcagt tgctgagcgt tgttcgtcag aggacagttg caggtcagat tcagggaaag
      181 cgagcatatt tgcctcgacc agggttgcgt atttccatcc ctcttccgag cgtcggagtc
      241 ggtactcact ctcgtaggaa agagtctggc tgacgtcata gaccgcattc agggttacca
      301 gaggctcggt cagatgtcct tcgacctgaa cacgaacacg acaagagcct tctggatcca
      361 ccttgtaggc aatcaagcgc gtttggttca cgacgaacag attgggattc gtgaccccga
      421 tcgccagtcc acccatctcg gtgatgatgg cacgtacctg ctcatcggtc ggtgggagga
      481 acggttcgac tttaacttca gagacattct gctctggcgg ttcggcagga ccgcaaccca
      541 agaggacaag aactatgaat agacacaaac tcaatctcat gatcactcag aaccgaaaga
      601 tccggacacg agactctgcc gcttgctttg gtctccgatt ctgatgcccc ctctctggag
      661 cgggtattag ccgtgggcgt ttgagctaag cccgtctttg tgtccttgtt tgagtcagaa
      721 accggaatcc ttgtggaatc cggtttcata aaagtttgtt ttgaaaaaga ctggcgaaag
      781 attatttcgt ccgaatcttg ttgatgcagt ccatgcacac acgcatcttg cggcggttgc
      841 cgccgccttg cgcaacacga atggtgtgaa ggttcggaaa gaaccgtcgt ttggttcggt
      901 tattggcgtg gctgacgttg tgtccgaatt gagcaccctt gccacatacc tggcacgctc
      961 gagccatgga aaacacacct cctcagtgcc ttacggcaaa agataggcac aaaaccttaa
     1021 caaatcgagc gtgtaagtta gacgtcttgg cggggaaaag caagcttttt cggaaaaacc
     1081 gaactttgta cagaaaaaag gtggagcagg agacgagatt cgaactcgcg acttccacct
     1141 tggcaaggtg gcattctacc actgaattac tcctgctcag tgacggcaaa agctatactt
     1201 caagcctctt gagattgtca agagaaaatc ttgtggctca gaaaatgatt gctttttcag
     1261 caagcctgag gaataatttg acaggaatgg gattgctcag ctgaatacta ttgttctttc
     1321 acagcattga gcggggtttt ctatggataa cagggatagc ccctgtacgt ccgacacaac
     1381 aatcaatacg tgtgggcaat tctctccagt tttcgaaggc aggccagcgc ctgcaggaaa
     1441 tgaggcagtg tgctcaggtt cccattctcc tttggaggat gggattgcag tgaggggtct
     1501 gcttcaggac ggtcaccctc acttggcgct tgaaggagtc ctgcaatgtc tccaacgcgc
     1561 actatcggtc gaagaacacc aagccgaatc gggcaccaac cgttttccat tctgttggtt
     1621 gaggataatc tggatcatgc cgagttgatc cgtcgcggtt ttggagccca tcacattgag
     1681 gatgcaattg tccatgttcg ggacggtgaa gaggcactgg actatctcct gtgccatggc
     1741 aagtacgccc aacgtaaaga cagggacaat cctcacctga ttcttttgga tctacggctt
     1801 cccaagattg acggtctgga agttctgaaa gtgatcaaga acacggaagg tatttgccgc
     1861 attcccgtcg tcatcctgac aacatccgat gcggagaggg atgtttctca ggcgtacgac
     1921 cgtcgagcca acagctatgt gatcaagcct ctggatttcc agacattcat ccagctgacc
     1981 aaggatttgg ggctgtactg gctgggttgg aatcttcatc cgtgctgata ggggggaaca
     2041 tcttggcaca gagcatttcg gttgacagtg ggctgggggt atctttgcga ggacacctgc
     2101 cataccagat tctcctggtt gaggacgaac ccgaccatgc ggagttgatc cgccgttcgc
     2161 tcaactcagg cc
//
LOCUS       DAOQUC010000691         2170 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_63633_length_2170_cov_2.656738, whole genome shotgun sequence.
ACCESSION   DAOQUC010000691 DAOQUC010000000
VERSION     DAOQUC010000691.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2170
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63633_length_2170_cov_2.656738"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1634)
                     /locus_tag="PKH07_11535"
     CDS             complement(<1..1634)
                     /locus_tag="PKH07_11535"
                     /inference="COORDINATES: protein motif:HMM:NF017078.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE4 domain-containing protein"
                     /protein_id="HNT35618.1"
                     /translation="MVTISLRTKILLILLGIFAVYALLNYGVQHYVIYPRFVLLEEEE
                     AKRNMNRAIQALQREIHGLEWFCHDWSAWDDIYIYAQDRNEAFAETNLTPTLLADYKL
                     DIVYIASATGDRIWSRDIRMTSEGYTPLEEFPTEGISVEHPIMSVLKAPGPYERVTVN
                     GIIQTSAGPLLFASRPIVSSQHEGPPSGVLIMARFLDAVRIKTLEEQTQLKISIRHLA
                     DPNIPPQGKSALSRLKGTTSVPYLKASATRQYIFEVFPDYSNHPCLLIEVSFPRHITN
                     QGRLATLFATVSLLIGGLIGLLVLMTLLHKAIVSPVIQLRDHMVRVGETDDLSAKLAS
                     DRDDEIGVLAREFDRMVGHLADARKKLLEHSYKVGKAEVAAEVLHNVRNSLSPISGAI
                     TFLRDRIRNTHFERIEAVKSELAQSSVDPQRAKDLNQYLVIFCDRVCAFLRESQGKLV
                     EIDEDIVRIEQLLAQQDAESHISERPKERVQLGDIVQDAVAKTSFAIQEFLRYHLSVD
                     DEARSQVIRTDRNALVQIIACLLSNASESVQKLSEG"
     gene            2075..>2170
                     /locus_tag="PKH07_11540"
     CDS             2075..>2170
                     /locus_tag="PKH07_11540"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNT35619.1"
                     /translation="MRNRRLGFTLIELLIVVAIIGILAAIAIPNFL"
BASE COUNT          529 a          559 c          534 g          548 t
ORIGIN      
        1 tcaccttctg acaatttctg cacagactca gatgcattgc tgagtagaca ggcaatgatc
       61 tgaaccagag catttctgtc ggtgcgaatc acctgagacc gcgcctcatc gtctacactc
      121 agatgatacc gcaggaattc ctggatagca aaactagttt tggcaacggc atcttgtacg
      181 atatcaccaa gttgaacccg ttccttgggt ctctccgaga tgtgggattc cgcgtcctgt
      241 tgtgccagca gttgttcaat ccgaacgatg tcctcgtcaa tctcaaccag tttcccctgc
      301 gactcccgaa gaaaagcaca gaccctgtca caaaagatga cgagatattg gttcagatcc
      361 ttagctcttt gcggatcaac actgctctgc gccaactcgc tcttgaccgc ctcgatacgc
      421 tcaaaatgag tgttcctaat tcgatcccga agaaaagtga tggctccaga gatcggactg
      481 agcgaattgc gcacattgtg caggacttcg gcggccactt ccgctttccc gactttgtat
      541 gagtgctcca gaagcttctt tcgggcatcg gcaagatgac cgaccatccg gtcaaactca
      601 cgtgcaagaa caccgatctc atcatccctg tctgaggcga gtttggcgga aagatcgtcg
      661 gtctctccca ctcgtaccat gtgatctctg agttggataa caggtgacac aatcgccttg
      721 tgaagcaggg tcatcaacac gaggaggccg atcagcccac caataagaag ggacacagtc
      781 gcaaacagag ttgccagcct gccttggttt gtgatgtgtc ttgggaagga gacttctatc
      841 aacaggcacg ggtgattcga atagtcggga aagacctcaa aaatgtactg cctggtcgct
      901 gacgccttca gatagggcac cgaagtggtt cccttcaacc gcgacagggc actctttccc
      961 tgagggggga tattcgggtc tgccaggtgc cggatgctga tcttcagttg tgtctgctct
     1021 tcaagggtct tgattcggac agcgtcgaga aaacgcgcca tgatgagtac tcccgagggc
     1081 ggtccctcgt gctgactact cacaatcggc ctggacgcaa acagcagggg gccggctgag
     1141 gtctgaatga ttccattgac agttacgcgt tcgtagggtc caggtgcttt caacaccgac
     1201 atgatgggat gttcgacaga aattccttcc gttggaaact cctcaagcgg agtatatcct
     1261 tcggaagtca tccggatatc acgtgaccaa atccggtcac cggtggcact ggcaatgtag
     1321 acaatatcca gtttgtagtc tgccagaagg gtcggagtca gattggtttc ggcaaaagcc
     1381 tcattccgat cctgagcata gatgtaaatg tcatcccagg cagaccagtc gtgacagaac
     1441 cactcaagac catggatctc tctttggagg gcttggatcg ctcgattcat gttccgcttg
     1501 gcttcttctt cttcgagcag aacgaacctg ggatagatga catagtgttg aactccgtag
     1561 ttcagaagag catacacggc gaaaatgccg agaaggatga gaagaatctt cgttctaagt
     1621 gaaattgtca ccattttccc tatccaagta tctatcgtgc ctctcacgtc tccctttgtc
     1681 ccatcttccc cagaaagaac gcaaaccgga gcaacaagcc cccgaaatac aattcacgct
     1741 atccttcgag cattctacca tcagcaggcg cgtgggctat acccactcac gctgatgctt
     1801 gctcggcaga cggcagacct ctttaggact cagtgtcaat ttgggatagt ctaccaccgt
     1861 ctgagagaaa aatgccaatg atttttttgc ttgcaccaat gcatggaact ccatacaatg
     1921 aatccggtgg gtagagaatc tttcaattct gggaaagcga ggtgatgctt cgccggattt
     1981 tgtctttcgc atgccttttg aggcaatagc aactcagcag tttttttgag tcaagtttgt
     2041 tggatttttc tttctattat ttgaggagca agagatgaga aatagaagac ttggttttac
     2101 gttgattgaa ctgctcattg ttgtggcgat catcggcatc ctggcagcca tcgccatccc
     2161 caacttcctg
//
LOCUS       DAOQUC010000692         2165 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_63863_length_2165_cov_3.240758, whole genome shotgun sequence.
ACCESSION   DAOQUC010000692 DAOQUC010000000
VERSION     DAOQUC010000692.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2165
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63863_length_2165_cov_3.240758"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(123..491)
                     /locus_tag="PKH07_11545"
     CDS             complement(123..491)
                     /locus_tag="PKH07_11545"
                     /inference="COORDINATES: protein motif:HMM:NF013427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HNT35620.1"
                     /translation="MPKKVQKETLNGKRLNELKEMLLNEKALVLKHSARELGGEGGVA
                     HGDMADLTTEIAEREQLLRLAEHDRERLRLIDEALEMVDNGQYGTCNECGVAIPEPRL
                     KALPTASLCVTCQATVERRG"
     gene            778..1461
                     /locus_tag="PKH07_11550"
     CDS             778..1461
                     /locus_tag="PKH07_11550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35621.1"
                     /translation="MEAVPFVIFVLVIIVFIVLSAYFAGKRRKELMEWAHSKSLSFEP
                     SKHKNIDDQYPDFKCFRQGSNRYAENVMRGSWSEKPILAFDYHYETHSTNSKGQRQTH
                     HHRFSAIIVGSDLVLKPLFIRPEGFFDKITEFVGFDDIDFESAEFSRKFYVKADDRRW
                     AYDVIHQRTMEFLLDRPQFTIQFAGRDVLVHRSSRFSIQQFEQALEIASGILDRLPDY
                     LVKQMKEGN"
     gene            1465..2049
                     /locus_tag="PKH07_11555"
     CDS             1465..2049
                     /locus_tag="PKH07_11555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HNT35622.1"
                     /translation="MNELRLENMMPVLIVVGIFVLIPLIWVIGTYNGLIRLRNHCRES
                     WSGIDTELKRRYDLIPNLVETVRGYAQYEKDVLERVVQARSRAVASTGSPQTQAQDEN
                     ALVGSLRSLLVVVEKYPDLKANQNYLHLQNELVNTEDRIQAARRFYNANVRDLNTRIE
                     VIPSNLVATLFGFQKEEFFEIESACERSVPSVKI"
BASE COUNT          502 a          594 c          518 g          551 t
ORIGIN      
        1 agtcgtttcc acttctcgaa gccacgctca tctctcgtac ggtgaagggc agtcggatca
       61 accgttgcac tcaagactca gcccatgtgg caatgacgag tgcgttccgg tatgcttgcc
      121 aatcatcccc gacgttccac tgtcgcctga caggtcacgc aaaggcttgc tgttggcaga
      181 gcctttaagc gaggctcagg aatcgccaca ccgcattcat tgcaggtgcc atactggccg
      241 ttgtccacca tctccaaagc ttcgtcaatc agacgcaggc gctcccggtc atgctccgcc
      301 agtcggagca actgttctct ctcggctatt tccgtggtca gatcggccat atcaccatgc
      361 gcgactcccc cctcaccgcc cagttccctg gcactgtgtt tcaagacaag cgctttttcg
      421 ttgagtaaca tctccttcaa ctcattgagt cgcttgccgt taagtgtttc tttctgaact
      481 ttcttgggca ctggtctggc ctcctcaatc cgttagtgta ctcccttttt cgaggagaac
      541 aactgattct tcttcttaga gatgccattc agtcaaagcc tcgcgaccga cacaagtcag
      601 tcatcaagtg ccccgactga acccatctga aggcgtaata catagaacca accgattgga
      661 attgtcaact tttttcgcat tttgccactt ttggacccag aagcacttgt gcaatatttc
      721 acaagttgcc atcctcacac ctctgcggta gagtgtcttt cactggaggt tgaacccttg
      781 gaggctgttc catttgtcat ctttgttttg gtcatcatcg tctttattgt gctcagcgcc
      841 tacttcgcag ggaagagacg gaaagaactc atggaatggg cacattccaa gtcgctttca
      901 ttcgagccct cgaaacacaa gaacattgat gatcaatacc cggatttcaa gtgtttccga
      961 caaggctcca atcggtatgc ggagaatgtg atgcgaggct catggtctga aaaaccgatt
     1021 ctcgcttttg actaccacta tgagacccat tcgaccaact ccaaagggca gcgacagacg
     1081 catcaccacc gcttttccgc catcatcgtt gggagtgatc tggttctaaa accattgttc
     1141 atacgtcctg aggggttttt cgacaaaatt accgagttcg tcggcttcga tgacattgac
     1201 tttgagtcag ccgagttcag tcggaaattc tacgtcaaag cggacgacag gcgctgggca
     1261 tacgatgtca tccaccagcg tacgatggag tttcttttgg atcggcctca gttcaccatt
     1321 cagtttgccg gtcgcgatgt tctcgttcac cgttcctcac gtttttcgat acagcagttc
     1381 gaacaggcgc tggagattgc ctcaggcatc cttgatcggc tgccggacta ccttgtcaaa
     1441 cagatgaagg aaggaaacta gaaagtgaat gaactgcgat tggagaatat gatgcccgtt
     1501 ctcattgttg tggggatctt tgttctgatt cctctgatct gggtcatcgg cacttacaac
     1561 ggcctgattc ggttgagaaa tcactgtcgg gagtcctggt caggtattga taccgaactc
     1621 aagcgacgct atgacttgat cccaaatctc gtcgaaacgg ttcggggcta tgctcaatat
     1681 gaaaaggacg tcttggagcg ggttgtccag gcacgcagtc gtgccgttgc ttccaccggg
     1741 tcgccccaga cacaggcaca ggacgagaat gcacttgtag gctcactcag gtctcttctg
     1801 gtggtcgtgg aaaagtatcc agatttgaag gccaaccaaa actatctcca tctgcaaaac
     1861 gaactggtga acacggaaga ccgcatccag gccgcgcgac gcttctacaa cgccaacgtg
     1921 cgagacctca acacacgtat cgaagtcatc ccctccaatc tcgtcgccac gttgttcggt
     1981 ttccagaaag aggaattctt cgagatcgaa agtgcatgcg aacgctcggt accgtcggtg
     2041 aagatttgac ggagaccaga acggttcaaa aggcaggcaa atcccccctt cgcccataag
     2101 cgctaacctt agtcgagtag ggtggaacga gtctgcgagt tccaccactc cgaatgtttt
     2161 caccg
//
LOCUS       DAOQUC010000693         2162 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_63986_length_2162_cov_3.099668, whole genome shotgun sequence.
ACCESSION   DAOQUC010000693 DAOQUC010000000
VERSION     DAOQUC010000693.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63986_length_2162_cov_3.099668"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1737)
                     /gene="mnmG"
                     /locus_tag="PKH07_11560"
     CDS             complement(<1..1737)
                     /gene="mnmG"
                     /locus_tag="PKH07_11560"
                     /inference="COORDINATES: protein motif:HMM:TIGR00136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HNT35623.1"
                     /translation="MKFDVIVVGAGHAGCEAASASARLGLETLLVTLDLDKIAQLSCN
                     PAIGGSAKGQITREIDALGGIQARITDATAIHFRMLNTRKGPAVHAPRAQVDRWEYQK
                     VMKLVLENTTKLHLYQAETVALVVEQGIVGGVFLRTGECIKASAVVLAPGTFLRGLIH
                     VGLNSYEGGRAGDLPSVPLADNLRQLGFKVGRLKTGTTPRLDARSIAFESLEEQPGDE
                     PPGHFSFFGKEKIRNRVRCHITWTNPATHEIIRHNLDRSPLYTGKIKSRGPRYCPSIE
                     DKVMKFPERERHQVFLEPEGAESQEVYANGLSTSLPVDVQQSYLQTVPGLENAVILRP
                     GYGIEYDFVEPTELKHTLETKAVTNLYLAGQINGTSGYEEAACQGLIAGVNAARKILG
                     REEIVLNRSDAYIGVLIDDLVTKGTQEPYRMFTSRAEYRLILRQDNAILRLGQMAEEI
                     GLLTSEEVTHSHQLRDAIFSERKRLDETTVRPTEEGNRLLTEAGADPMHEPVKASQLL
                     RRPQVYYGWLPVFGYDISHVPNAVREQVEIEVKYEGYIKRQQTQIAEFFKIEHISITD
                     GLLSNPIAGIRRE"
     gene            complement(1766..>2162)
                     /locus_tag="PKH07_11565"
     CDS             complement(1766..>2162)
                     /locus_tag="PKH07_11565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35624.1"
                     /translation="DQIQQQLENLQQRLASASGLEDKLEELKEEQRAQQESSQTKEQA
                     SSATNKNMTDPGAGGSDQEQGNDETNGPRYRLEGFKMDFTGIGDVDERGDSSFQGSDD
                     LRAWEDEKVPAQYKSVVKRYFESLSSKEK"
BASE COUNT          474 a          594 c          523 g          571 t
ORIGIN      
        1 ttcccttcta atgcctgcga tgggattgct caagagacca tcagtgatgg agatatgctc
       61 aatcttaaag aactcggcaa tctgcgtctg ctgccgcttt atgtagccct catacttaac
      121 ctcaatctcg acctgttccc gcaccgcatt gggcacatgc gatatgtcat atccaaacac
      181 tggcagccaa ccatagtaga cctgtggccg tctaagcagc tgcgacgcct tcacgggttc
      241 atgcatcgga tctgccccag cctcggtgag caaacgattg ccctcttccg tgggtcggac
      301 agtggtctca tcaaggcgct ttcgttccga gaagatagcg tctctgagtt ggtgggagtg
      361 agtcacttct tcagacgtaa gcaggccaat ctcctcagcc atttgcccaa ggcgcaggat
      421 ggcgttgtcc tgccgcagga tcaggcggta ctccgctcga gaagtaaaca tgcgatacgg
      481 ttcctgcgta cctttggtga caagatcatc tatcagaacc ccaatatatg cgtcggaacg
      541 gttgagaacg atctcttccc ttcccaggat ctttcgggct gcattcactc ccgcgatcag
      601 gccttggcag gcagcttcct cgtaaccgga cgttccatta atctgacccg caaggtaaag
      661 gttggtgact gcttttgtct ccagtgtgtg tttgagttca gtgggctcaa cgaaatcgta
      721 ttcgatccca tatccaggcc gaaggatgac tgcgttctca aggcctggaa ccgtttggag
      781 ataggattgc tgaacgtcaa ctggtaggct cgtagacaag ccatttgcat agacttcctg
      841 agattcagca ccttcaggct caagaaagac ctgatgtctc tcgcgctccg ggaatttcat
      901 gactttgtct tcgatggaag gacaataccg agggcctctg ctctttatct tcccagtata
      961 aagtggggat cgatcaagat tgtgcctgat aatctcatgc gtcgctgggt ttgtccaggt
     1021 gatatgacac ctgacacgat ttcttatctt ctctttccca aagaacgaga aatgtcccgg
     1081 tggttcatca cccggctgtt cttcaagact ctcaaatgca atcgagcggg cgtccagacg
     1141 tggagtggtc ccagttttca gacgtccgac cttgaaacca agttgtctaa gattgtccgc
     1201 gagaggtact gacggaagat cccccgcgcg ccccccttcg taagagttca gaccaacatg
     1261 aatcaaacct ctgagaaatg tccctggtgc gagaacaacc gcagaggcct tgatacactc
     1321 acctgtcctt aggaaaaccc caccgacaat tccctgctct actaccaaag caaccgtttc
     1381 ggcctgatag agatggagtt tcgtagtatt ctcaaggaca agtttcatga ccttctgata
     1441 ctcccagcga tccacctgtg cccttggggc atgtacggca ggccccttgc gtgtgttaag
     1501 cattctgaaa tgaattgctg ttgcatcggt gatgcgcgcc tggattccgc cgagtgcgtc
     1561 aatctcgcgt gtaatctgtc ccttggcgga tcccccgatg gcaggattgc aggacaactg
     1621 ggcgatcttg tccagatcaa gagtaaccag gagtgtctca agccccagcc ttgcactggc
     1681 gcttgctgct tcacatccag catggccagc gccgacaaca attacatcaa acttcatagt
     1741 gctcaatgag gtagtccagt gtgttctact tttcctttga ggagagagac tcgaagtacc
     1801 ttttcacaac tgacttatac tgtgctggta ccttctcatc ctcccaggca cgaaggtcat
     1861 cagaaccttg gaaagaggag tccccccgct cgtcaacatc tccaatgcct gtgaaatcca
     1921 ttttgaaacc ctccaacctg tatcgaggac cgttggtttc atcattgcct tgttcctgat
     1981 cggatcctcc cgcaccagga tccgtcatgt tcttgttggt tgctgaactt gcttgctcct
     2041 tggtctgtga agattcctgt tgagctctct gttcttcttt gagttcctcg agtttgtcct
     2101 cgagtcctga tgcagaagca agcctttgtt gcaggttctc cagctgctgt tggatctggt
     2161 ca
//
LOCUS       DAOQUC010000694         2162 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_63996_length_2162_cov_2.657807, whole genome shotgun sequence.
ACCESSION   DAOQUC010000694 DAOQUC010000000
VERSION     DAOQUC010000694.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63996_length_2162_cov_2.657807"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1758)
                     /gene="fliD"
                     /locus_tag="PKH07_11570"
     CDS             complement(<1..1758)
                     /gene="fliD"
                     /locus_tag="PKH07_11570"
                     /inference="COORDINATES: protein motif:HMM:NF018852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HNT35625.1"
                     /translation="MSNYSVGGLISGFDTESIVQSMMDVERIPVKKLEIRKASQLSLV
                     TAYQAMSARLIGLKVPVDNLLKPTTFQGRTVTSSDDKIVSATVSNAALANAHHITNIQ
                     MAQAHRIAGTTFSDATTKLNLEGDVLINGSVVRVAKDDTLTDIAARINSTVSNVSASV
                     LSVSDTDKRLVLTSKQTGTDSSLDLRDANDSGILKTLGLTSSTMTVRNPIADGANSGL
                     FAVKTTSIGSLLRLSSPAVSGTIQILDDNGSLSVSVDLATDSIESIAQKINDAGHASI
                     SASVVEETNGGVTRFRLQITGAVTPAGEPFSASNFVDDNGILESLGILRAGYGQELTE
                     ARNATFDLDGITMTRSSNLVSDAITGMTFSLLKDSTETVTLSVASDQSPTVSAVQSFV
                     DNYNATLSYIKEQTFYNKETGSKGLLLSDNTTRGIRNLLASMIGRQVPMLSMQNLTSL
                     NDGSGVSSGSIKITNRADQSATIDLSRAVTVQDVLIAINSAGIRVTASVAPNGTGILL
                     TDTSGGSGSMKVEEVDGGATAADLGILGNTYSSTLYGKAVADAQFKSLSDIGISLQTD
                     GTLSLNATTLRKALESDQDK"
     gene            complement(1868..>2162)
                     /locus_tag="PKH07_11575"
     CDS             complement(1868..>2162)
                     /locus_tag="PKH07_11575"
                     /inference="COORDINATES: protein motif:HMM:NF015602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar protein FlaG"
                     /protein_id="HNT35626.1"
                     /translation="METGTRAPQASPQEVVEPKLTAANADQVMKAISDLQSAMETLHR
                     KINFDVDQGSGRFHVKVIDTATDEVIREIPPEQVLKIAARMKELIGLLFDETV"
BASE COUNT          461 a          563 c          612 g          526 t
ORIGIN      
        1 tttgtcctga tcgctctcga gcgccttgcg gagcgttgtt gcgttgagcg acagtgtgcc
       61 gtcggtttga agactgatgc caatatcgct cagactcttg aactgggcat cggcaaccgc
      121 ttttccatag agagtgcttg aataggtatt acccagaatg cccaggtcag ccgctgtggc
      181 cccaccgtcc acttcttcga ctttcatcga gcctgagcca ccacttgtgt ccgtcagcag
      241 aatgcccgtt ccattgggcg cgacgctcgc ggtgactcga atgcctgcgg agttgatcgc
      301 gatcagcaca tcctgaacgg tgacggctcg agaaagatca atggtagcgc tctggtcggc
      361 tcggttggtg atcttgatcg aaccggacga gactccggag ccatcgttca gggaagtcag
      421 attctgcatt gagagcattg gaacctgacg gccgatcatg gaggccagca ggtttcggat
      481 tcctctcgtt gtgttgtccg aaagcagaag acctttgctt cccgtttcct tgttgtagaa
      541 agtctgttcc ttgatgtagg acaaagtggc gttgtagttg tctacaaagg actgtaccgc
      601 agacacggtt ggtgactggt cggaagcgac agagagggtg accgtctccg tggaatcctt
      661 gagaagggaa aaggtcattc cggtgatcgc gtcagagacg agattcgaag accgagtcat
      721 cgtgatgccg tccaggtcga aggtcgcatt tcgtgcctcg gtcagttctt ggccgtaccc
      781 ggcacgcagg attccaaggg attccagaat tccgttgtcg tccacgaaat tcgacgcgct
      841 gaagggctca cctgccggtg tgaccgcgcc ggtgatctga agtcggaacc gggtcactcc
      901 gccgtttgtt tcctcaacca cagacgcgga aatgctcgca tgacccgcat cattgatttt
      961 ctgagcgatg gactcgattg aatcggtcgc caggtctacc gatacggaca gagaaccatt
     1021 gtcatctaga atctggatcg tgcctgaaac ggcaggactg ctcaggcgaa gaagactgcc
     1081 gatcgacgtc gtcttgactg caaagagacc gctgttcgct ccatcagcaa ttggattgcg
     1141 aaccgtcatc gtggatgaag tcaggccaag agtcttgagg attcctgaat cattggcatc
     1201 tctgagatcg agtgacgaat cagttcctgt ctgcttgctc gtgagcacca aacgtttatc
     1261 tgtatcggaa acagagagca cagaggccga aacgtttgaa acggtgctgt tgatacgagc
     1321 tgcaatgtcc gtgagcgtgt catctttggc cacccggacg acgcttccat tgatgagcac
     1381 atcaccttcg aggttgagtt tggtggtcgc atcagaaaag gttgtgccgg caattcggtg
     1441 cgcctgtgcc atttggatat tggtaatgtg atgggcattc gccagtgcgg cattggagac
     1501 tgtggcagaa acgatcttgt catctgaact ggtgaccgta cgtccctgga aagtagtcgg
     1561 cttaagaaga ttatcaaccg gtaccttcaa gccaatgagt cgggcgctca ttgcctgata
     1621 ggcagtgacg agagacagtt ggcttgcttt ccggatttcc agtttcttga caggaatccg
     1681 ctctacgtcc atcatggact gaacgatgga ctccgtgtcg aaacctgata tcagaccacc
     1741 taccgagtag ttcgacatct ccgttcacct cgccaacagt ctgggctggg ctcgtccgca
     1801 cgaggcgggc gacgctccgt ccgcaggtca ttcccaagaa gccttggggg caggggaagg
     1861 agcgccgtca aaccgtttcg tcgaacagta gcccgatgag ctccttcatc cgagccgcga
     1921 tcttgagaac ctgctcggga ggaatctccc tgattacctc atcggttgct gtgtcaataa
     1981 cctttacatg aaatcttccg gaaccttgat caacgtcaaa attgatcttg cggtgtagcg
     2041 tctccatggc gctctgaagg tcagatatgg ccttcataac ctggtctgcg ttcgccgctg
     2101 tcagctttgg ctcgacgact tcctgtggcg atgcctgggg agccctggta ccagtttcca
     2161 tt
//
LOCUS       DAOQUC010000695         2162 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_64007_length_2162_cov_2.067394, whole genome shotgun sequence.
ACCESSION   DAOQUC010000695 DAOQUC010000000
VERSION     DAOQUC010000695.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64007_length_2162_cov_2.067394"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2162
                     /locus_tag="PKH07_11580"
     CDS             <1..>2162
                     /locus_tag="PKH07_11580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35627.1"
                     /translation="IGIGTDANYAAVSMFCFQDQLPTNENSLLFEELALLRGRDGRDG
                     TRTDQFPVYNRLYWNFTSGDGQVAYVNNYNIQDFYPIFEDCRGDGILNEYDAQVMFPQ
                     GHGDAWGHYLSAMRFYYDLLRHPYFTWQVRSEAVVVNQQPVMISYAHERKFAKAAAAK
                     AKAGLEITSLAYREQYLEDPSEQWKGYPDPANRDWGVTDWARRSFMAAYFDWALGNAM
                     VPAKDEVHPPESLEKVDRTTVVQLHEIATTADQIQVQLDQADAGVNPLGLPKNVVPFD
                     INPSQLDPERIGGAITHFEQVYDKAVGVLNNTVNVFNHANQAAQRLRENQDDAAAWEN
                     NIEDGEWDYLCRLIEIFGYPYPEDKNPITGAVYGFDFEGPDLYHWMYVNVEDLLGMDL
                     PQGDTIKQTFTSYAASEIFGIGAVATATTSDVEFRVVPGFGFVKPKEFKYERKAPGEI
                     QLARSDLLQEWWRFKKAIKEYENSIKDVQEQVDTINSMAILRGVQIGIKTAHMTTVIG
                     LNAGIMAAKVLELGFKRAADLTDNTMNSLAESLPTVLGMACDVTSVARGAAKIGGGTA
                     SNVLNILGDVAEGVGFALELSKQIVAMGVDISLDVAKGVYENHAEVLKLQEKIRKMYT
                     QQIELYALQESMRQASGRYQKVIAEGARLIVERTAYRVALAEQISKYRYKDMAFRVFR
                     NDALQKYRAQFDLTARYAYLATKAYGYETNLLDFNSQS"
BASE COUNT          527 a          543 c          616 g          476 t
ORIGIN      
        1 cattggcatc ggcacggatg ccaactatgc ggccgtttcc atgttctgct tccaggacca
       61 gctgccaacc aacgagaact ctctgttgtt cgaagaactg gcgctgcttc gaggtcgcga
      121 cggtcgggat gggactcgca cggatcaatt ccccgtctac aatcgccttt actggaactt
      181 cacttctggt gacggtcagg tcgcctacgt caacaactac aacatccagg atttctaccc
      241 gatctttgag gactgccgag gtgacggtat cctcaatgag tacgacgcac aggtcatgtt
      301 cccacaggga cacggtgatg cctggggtca ctatctgagc gcgatgcgct tctactacga
      361 cctgttgagg cacccgtatt tcacttggca ggttcgttcg gaggccgtcg tcgtcaatca
      421 gcagccggtg atgatcagtt atgcccatga gcgcaagttc gccaaggccg cagcggcgaa
      481 agccaaagcc ggcttggaga ttaccagtct ggcctaccgt gaacagtatc tggaggatcc
      541 atccgaacag tggaagggtt atcctgatcc tgccaaccga gactggggtg tcacggattg
      601 ggcgcgacgc agcttcatgg cggcatactt cgattgggca cttggcaatg cgatggttcc
      661 tgccaaagac gaggttcatc cccctgagtc tctggagaag gtggacagga caactgttgt
      721 gcagttgcac gagatcgcca cgacggcgga tcaaattcag gtacagcttg atcaggcaga
      781 cgcgggcgtc aacccgttgg gactgccgaa gaacgtcgtg ccatttgata tcaatccatc
      841 gcagctcgat ccggagcgga tcggtggggc gattacacac tttgaacagg tctacgacaa
      901 ggcggtgggc gtcttgaaca acacggtgaa tgtgttcaac catgccaacc aagccgctca
      961 gcgccttcga gagaaccagg acgacgctgc tgcctgggaa aataacatcg aggacggaga
     1021 gtgggactat ctgtgtcgtc tgatcgagat ctttggctat ccgtatcccg aagacaaaaa
     1081 cccgattacc ggtgcagtct atgggttcga ctttgagggt ccggatctct accattggat
     1141 gtatgtgaac gtggaagacc ttttgggaat ggatttgccc caaggggaca cgatcaagca
     1201 gacgtttacc agttacgccg cttcggagat cttcggcatc ggtgctgtag ctacagccac
     1261 aacaagcgac gtggagttcc gagttgtgcc tggttttggt ttcgtgaaac caaaagagtt
     1321 caagtacgaa cgcaaagcac ccggcgagat ccaattggcg cgctctgacc tgcttcagga
     1381 atggtggcgt ttcaagaaag ccatcaagga atatgagaat tccataaagg atgttcaaga
     1441 acaagttgat acgattaata gtatggcaat tctcagaggg gtgcagattg gaatcaaaac
     1501 agcacacatg accacggtga taggtttgaa tgcgggcatc atggcagcaa aggtgctcga
     1561 actgggcttc aagagggctg ccgatctcac cgacaacacc atgaactccc tggctgaatc
     1621 acttccgaca gttcttggaa tggcttgtga cgttacatcg gtagcacgcg gagcggcgaa
     1681 gatcggaggt gggacggcat ccaacgtcct caatatcctt ggagatgttg cggaaggggt
     1741 tggatttgcc ctcgagctgt ccaagcagat cgttgcaatg ggagttgata tctccctgga
     1801 cgttgcgaag ggagtctatg agaaccatgc agaggtgctg aagcttcaag agaagattcg
     1861 aaagatgtac actcagcaga ttgagttgta cgccttgcag gaatccatga gacaggcatc
     1921 aggacgctat cagaaggtca tcgctgaagg tgctcgattg atcgttgagc gcaccgcgta
     1981 ccgagtggcg cttgccgagc agatcagcaa gtatcgctac aaggatatgg ctttccgagt
     2041 cttccgcaat gatgctctgc agaagtaccg cgctcagttc gatctcacag cacgctacgc
     2101 ctatctggca acaaaagcgt acggctatga gacgaacctt ctggacttca atagtcagtc
     2161 cg
//
LOCUS       DAOQUC010000696         2161 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_64019_length_2161_cov_4.149573, whole genome shotgun sequence.
ACCESSION   DAOQUC010000696 DAOQUC010000000
VERSION     DAOQUC010000696.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64019_length_2161_cov_4.149573"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            233..1459
                     /locus_tag="PKH07_11585"
     CDS             233..1459
                     /locus_tag="PKH07_11585"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNT35628.1"
                     /translation="MRHVLTMLLLVATCGFSSHAEEAADSVQSASQARHFNALGYDLY
                     VLGEHDEAINMYLKALDFEWNRKAVVYNNLGAAYYARKDYDEALRCYQKAVDLDATYS
                     KAKVNVAAAHFRLRHYWRAAHALRGVQQQDPKYVHRRLKPDKVREELNAENERNPGNP
                     FLLQLVSLADERVVESTPSLIQSPEVQTVAAAVNEHFAKLSTFSARVVRNTQSPQGTA
                     RQEWKVLLRLPDQIRVEWLGPQPMTFITSGTEAWYYTPAEKKAVHVPKVDPESLPKMD
                     LGLNVLPEYIQTHLFKISEDSPEGQWLLVGRPTSTSERVAEVRLWIDQSSKCLLRSEL
                     YDREGQIRVKTLYTRFRDCEQGLQLPGKCTILSYDRGACLESEMTFLELRANVPVQDE
                     NFRFLPPDGVEVQEQD"
     gene            1503..1859
                     /locus_tag="PKH07_11590"
     CDS             1503..1859
                     /locus_tag="PKH07_11590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012532031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35629.1"
                     /translation="MKKTKVTLVLLIVVMVATAGLSNAQVRGKQDYWNNKTHDPQASM
                     WAARLSCMFPGTGEWYNRDFRGEFPLAECILGSICPLVSLCSFFDAAAGDTSDKIRLD
                     FWSLTNNNSYVPSRGM"
BASE COUNT          530 a          547 c          590 g          494 t
ORIGIN      
        1 tcttcgcagc gcttctgaac ggcctgtcca tcggcttggc agtcgaagcc ttttaaaaag
       61 ggcgagtcag cacgggaatc gtcaccggtg ctatcgcatc cacattctat ttgggcaaca
      121 tctacggtgc acgcaacgct gccatccgct ataatcagca gcaggtgtat aactggatcg
      181 aacagatgaa gcagaacact cctgtggatc tattcggtga ggagttttcc gaatgagaca
      241 tgtattgacc atgcttctcc tcgtcgccac ttgtggcttc tcatcgcatg ccgaagaggc
      301 agctgactct gtccagtcgg cgtcccaggc acgtcatttc aacgcattgg gttacgatct
      361 ctacgtcctg ggcgagcacg acgaagcaat caacatgtat ctcaaggcgc tggatttcga
      421 atggaacaga aaggcagttg tctacaacaa ccttggcgca gcctactatg ccaggaaaga
      481 ctacgatgag gccttgcgtt gctatcagaa agctgttgat ctcgatgcaa cctactccaa
      541 ggcaaaggta aacgtcgccg ccgcgcactt tcgactgcgt cattactgga gagcggcaca
      601 cgctcttcga ggagtgcaac aacaggatcc gaaatatgtt catcgcagac tcaagccgga
      661 caaggttcgc gaggaactga acgcggaaaa cgagcgaaac ccgggaaatc cctttcttct
      721 ccagttggtg agtctcgccg atgaacgtgt ggtggagtcc acgccttctt tgattcagag
      781 tcctgaagtc cagactgtcg cggcggcagt caatgagcac ttcgccaaac tttcgacgtt
      841 ttctgcgcgt gtggtgagaa acacgcaaag cccccaggga actgcgcgac aggaatggaa
      901 agtcctgttg cgacttcccg atcagattcg cgtggaatgg ctcggaccgc aacccatgac
      961 cttcatcact tcaggaactg aagcgtggta ctacacccca gccgagaaga aggcggttca
     1021 cgtccccaag gtggatcctg aatcgctacc caaaatggac ttgggactga acgtccttcc
     1081 ggaatacatc cagacgcatc tgttcaaaat ctccgaggat tcaccggaag ggcagtggct
     1141 gcttgttggc agaccaacca gcacatcgga gcgcgttgcg gaggtgcgcc tttggattga
     1201 ccagtcttcc aagtgtctgc ttcgcagtga gttgtacgac cgggaaggac agatccgagt
     1261 caagactctc tacacgcgat tcagggattg tgaacaggga ttgcagttgc cgggaaaatg
     1321 tactatcctg tcttatgata gaggggcttg tttggaatct gagatgacat ttttggaact
     1381 gcgcgcgaat gtcccggtgc aggacgagaa ttttcgcttc ctgccaccgg acggagtgga
     1441 agttcaggaa caggactgac aagagtaata tgattgattg tccgaaaggg ggtgagaagg
     1501 taatgaagaa aacaaaagtg accttggtac ttctgatagt cgtcatggtt gcaacagccg
     1561 gactgtcaaa tgcacaggtt cgaggcaaac aggattactg gaacaataag acacatgatc
     1621 ctcaggcttc catgtgggca gcacggttgt cctgtatgtt cccaggaacc ggagaatggt
     1681 acaacaggga ctttcgagga gagttcccgt tggcagagtg catcctcggc agcatttgtc
     1741 ctctggtctc gctgtgttcg ttctttgacg ccgcagcggg tgacacaagc gacaagatca
     1801 gactcgactt ctggagtctg accaacaaca acagttatgt gccgtcccgc ggcatgtaag
     1861 ggatttctca tcgcttatca ggccggataa cgttctggat gattccagca acgctttccg
     1921 gcctttcctg ttgagcaggt cagcgccgac aggcggtttg cgatgtccac ttcttcagac
     1981 gcctgctgaa tcaatgacaa ctggcaccgc actatgtcta tcgcaaatcc ctcctattgg
     2041 atcccaaggc ctcgtgacaa gatgaatgaa ctatgataca gtttcacctg ttgtcattgg
     2101 ggagaggtcg gggcatcagg cagtgctgga ggacggatgt aatgagcaga ttgattcgct
     2161 a
//
LOCUS       DAOQUC010000697         2161 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_64024_length_2161_cov_3.386515, whole genome shotgun sequence.
ACCESSION   DAOQUC010000697 DAOQUC010000000
VERSION     DAOQUC010000697.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64024_length_2161_cov_3.386515"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="PKH07_11595"
     CDS             <1..181
                     /locus_tag="PKH07_11595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35630.1"
                     /translation="TTPLSSRRASRTFVLPWHNGSVQPQESEKGKGGRFTIHIPKSLN
                     QMTSGAGSSTSLLGY"
     gene            511..1335
                     /locus_tag="PKH07_11600"
     CDS             511..1335
                     /locus_tag="PKH07_11600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012466537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HNT35631.1"
                     /translation="MRTMLVSLFAATGLMVSLAFAQETRIVIDGSTTVGPVAKAFAEY
                     YMSRNQGVNITVSESGSGNGAKSLINGVCDIASMSRFMKDTEFKEAADKGLMPVAHIV
                     AMDGLAVIVHPSNPVAQLTMEQIRDIFTGKIQSWKEVGGPDIKIVKISRDTNSGTYET
                     FETLVMNKEKIAADSEYVGSNGAVRQRVQSTAAAIGYAGLGFSDDTVKILEVNGIVPD
                     TDTVASGTYPIARPLYLFTNGYPQLGSHLHKFVSLYLTPQGQEIVVASGFVPVTKY"
     gene            1381..>2161
                     /locus_tag="PKH07_11605"
     CDS             1381..>2161
                     /locus_tag="PKH07_11605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease"
                     /protein_id="HNT35632.1"
                     /translation="MNPKPVQNKSQGFVLSKRASRFRRLGSLAGESLLLLITSTSALA
                     VLFIFYFIAKEGLPFVRLCGLRDFFTSTKWYPTAQPPMFGALAIFVGTGLVTLGAVFV
                     AVPLGLAAAVCLSDVLPFSLRQVVKPIIEILAAIPSVAYGFFALVVFAPLLQDHGGRI
                     IAGVFGLIGIPTAFLCVIVISDLLASRFEKPQQNLIRVVSWTVLGVSASFVIYALLKR
                     FASLSVSSGANALNVSIILGIMAIPTIVSVAEDALQSVGREL"
BASE COUNT          497 a          555 c          572 g          537 t
ORIGIN      
        1 aacaacgccg ttatcctcaa gacgggcgag taggacattt gtacttccgt ggcacaatgg
       61 ctccgtgcag ccccaggaaa gtgagaaggg aaaaggcggc cgtttcacca ttcatatccc
      121 gaagtccctc aatcagatga ccagtggggc aggttcttcc acctctcttc tcggatatta
      181 ggaaagactt ccttgggtaa tctgccgatc tcaatagacc cacaccactt cttcactcca
      241 taactcgggt gcctgaaaca gtaatgccac atctgctgac ttctggagtc tgagcgagat
      301 ctgcagttgg cagggaaaga gtgctcccca ctttttgctg gttcctaaca cacccctaac
      361 acaactctaa cacttttcag ccttactact agatgatgaa gacaaagagg aggtgcctga
      421 aggcagagtt tgccaggacg aggtttgaac agatcactaa caaacgaaac ggggcgccaa
      481 atcgccccag ttgaaaagga gaagaatctc atgagaacaa tgctcgtttc tttgtttgcg
      541 gcgactggcc tcatggtttc tttggctttc gcccaagaga cccgaattgt gattgacggc
      601 tcgacaacag tcgggcccgt cgcaaaggcg tttgcggagt actacatgtc ccgcaaccag
      661 ggcgtcaata tcacggtcag tgagtccggc agcggcaatg gtgccaagag cctgattaac
      721 ggtgtctgtg atattgccag catgtctcgc ttcatgaaag acaccgagtt caaggaagcg
      781 gcggacaaag gattgatgcc tgtcgcgcat attgttgcca tggacggtct ggctgtgatc
      841 gttcatccga gcaaccctgt ggcgcagctg accatggagc agattcgtga catctttacg
      901 ggcaagattc agagttggaa agaagttggg ggtcccgata tcaagattgt caaaatcagt
      961 cgcgacacca acagtggcac ctacgagacg tttgaaacgc tggtaatgaa caaagagaag
     1021 atcgctgcag attcagagta tgtgggcagc aacggagccg ttcgccagcg cgttcaaagc
     1081 accgcagcag ccattgggta cgctggtctg ggattctccg atgacacggt gaaaatcctg
     1141 gaagtcaacg gtattgtccc cgatacagac acagtcgcct cgggaaccta tcccatcgcc
     1201 cgtccgttgt acctgtttac gaacggttat cctcaactgg gcagtcatct tcacaagttt
     1261 gtctcactgt atcttacacc gcaagggcag gaaatcgtcg ttgccagtgg tttcgtgccg
     1321 gtgacgaagt actgacccga gagaggagca tcctttggtc agggaatttg ttgcttgagg
     1381 atgaatccga aaccagtgca gaacaagagt cagggtttcg ttctgagcaa gagagccagc
     1441 cgcttccggc ggcttggctc tcttgcaggt gaatccttgc tcttgctgat tacatctacg
     1501 tcggcgcttg ccgtcctgtt catcttctac tttatcgcca aagaaggtct gcctttcgta
     1561 agactctgcg gactgcgcga tttcttcaca agcaccaaat ggtacccgac agcgcaacca
     1621 ccgatgttcg gggcgctggc gatctttgtt ggaactggcc tggtcactct aggagccgtg
     1681 ttcgtcgctg ttccattggg cctggcagca gcagtttgtc tcagcgacgt gcttccattt
     1741 tccctgagac aagtggtcaa accgattata gaaattctgg ccgcgattcc ttcggtagcg
     1801 tacgggtttt ttgcgcttgt ggtctttgct cctctccttc aagaccatgg aggcagaatc
     1861 atcgcaggcg tctttggact cattgggatt ccaacagctt tcctgtgtgt catcgtaatc
     1921 agtgatctgc ttgcgtccag atttgagaaa cctcagcaga acctgatccg tgtggtgagt
     1981 tggaccgtcc tgggtgtgag tgcttcgttt gtcatttacg ctcttctaaa acgattcgca
     2041 tcgctcagtg tttcaagtgg tgccaacgct ctgaatgtct cgatcatcct cggtatcatg
     2101 gcaattccaa ccatcgtcag tgtggcagag gatgcgcttc agtcggtggg cagagagctt
     2161 c
//
LOCUS       DAOQUC010000698         2161 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_64038_length_2161_cov_2.820988, whole genome shotgun sequence.
ACCESSION   DAOQUC010000698 DAOQUC010000000
VERSION     DAOQUC010000698.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64038_length_2161_cov_2.820988"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2161)
                     /locus_tag="PKH07_11610"
     CDS             complement(<1..>2161)
                     /locus_tag="PKH07_11610"
                     /inference="COORDINATES: protein motif:HMM:NF024204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HNT35633.1"
                     /translation="QLRLIELQDQRLELSDIDTEVKALVAGVDSATTEKQIRALEEAI
                     RDLVQTNRTLLDSLITEHDRYFAALVDLDTAERLLAAETRAFVEFIKGQVLWVRSAPT
                     LRLGNLTEIVDASVWLLRPRHWAETMETIGSDFMDEPEMVLLGLVCVLALFRIRQKSR
                     SFLQKQSQNEIGEVSDRFVPSLYALGCTLLIASPWPALAALVQMRLSRAAGSTDFVLC
                     VASGLRSIVLPWFLLEFLRQVFRKQGVGECDLGWKPEGTAVLYRRLLWLMALVLPMTF
                     IRGTLTWHDSQIWEDALGRVAFLVSVLTLIVFAVRFLKPSSPLFNDWMTRRKDNAFAR
                     TRFIWYPLLVFVPVVLVFLSLAGYHYTAQQLHQRLMLSVILIAGLLFANALALKWLFL
                     IRCRLAKQRAAQAAAIREQSEQTDQHPKDAPKESASAQGESQEPQSETPVSSQATVEQ
                     ASIGIQTINIQTRRLIRSLFLFSLVMGLWFIWVDMLPALGVLTRIELWTRTVQVPAEA
                     VGAASDATATVGKIVTTSLADLLLALLILVLTFVAGRNIPTLLEAFVFHRLAIDNSIC
                     YAIRAVTRYLITITGVIVAFGTLGIGWSQVQWLAAAITVGLGFGLQEIFANFVSGLLI
                     LFERPMRVGDVVTVNDVTGTVSRIQIRATTITNWDRKELIVPNKKFITEQLINWTLSD
                     AILRINIPVGLAYGSDTNLARETLLRVAREHPNVLEDP"
BASE COUNT          551 a          618 c          556 g          436 t
ORIGIN      
        1 ggatcctcaa gcacgttcgg atgctctcgt gccactcgaa gcagtgtctc tcgcgccaga
       61 ttcgtgtccg atccataggc aaggcctacc ggaatgttga ttcggagaat cgcatcggac
      121 agagtccagt tgatcagttg ttccgtgatg aatttcttgt tgggaacgat cagttccttg
      181 cgatcccagt ttgtgatggt tgttgctcga atctggattc ttgagaccgt tccagtgaca
      241 tcgttgacgg tgaccacatc tccaacgcgc atgggtctct cgaaaaggat cagcagcccg
      301 gagacaaagt tggcgaaaat ctcctgcaaa ccaaaaccga gacccaccgt gatggccgca
      361 gcaagccact gcacctgtga ccatccgatg cccaaagtcc caaaggcgac gatcacaccc
      421 gtgatggtaa tcaaatatcg ggtgaccgct cggattgcat agcagatgct gttgtcaatt
      481 gccagacgat gaaaaacaaa ggcttccaga agagttggga tatttcgacc tgccacgaat
      541 gtcagcacca gaatcagcag cgccagcagc aagtcagcga gactggtagt gacaatcttc
      601 ccaaccgtgg cagtggcatc acttgcagca cccacggctt ccgcaggcac ctgaaccgtt
      661 cgagtccaga gttcgatgcg cgtcaataca cccagcgcgg gaagcatatc cacccagata
      721 aaccaaagac ccatcacgag agagaacaag aacaggcttc gaataagcct tcgagtctga
      781 atattgatcg tttgaatgcc tatgctggcc tgctcgacag tggcttgtga cgagacggga
      841 gtttcgctct gtggctcctg tgactctccc tgtgctgatg ccgactcttt tggcgcatcc
      901 ttgggatgct ggtctgtctg ctcgctttgt tcccggatgg ctgccgcctg agcggcgcgc
      961 tgtttggcaa gacgacatcg aatcaggaac agccacttca gagcaagcgc atttgcgaac
     1021 aacaaccccg caatcagaat caccgaaagc atgagtcgct gatgaagctg ctgtgccgtg
     1081 tagtgatacc ccgccaaaga caagaagacc aacaccaccg gcacaaatac cagcaaggga
     1141 taccagatga aacgggttct ggcgaaagcg ttgtctttgc ggcgcgtcat ccaatcgttg
     1201 aacaagggac tggatggttt gagaaaacga acggcgaaga caataagtgt caaaacactc
     1261 accagaaaag cgactcgtcc aagagcgtct tcccaaatct gactgtcgtg ccaggtcaac
     1321 gtgccgcgga taaaagtcat cgggagaaca agagccatga gccacaagag gcgtcggtac
     1381 agaacggcgg taccctcggg cttccaaccg agatcacatt ccccgactcc ctgtttgcgg
     1441 aacacctgtc tcagaaactc caaaagaaac cagggcaaca cgatggatcg aagccccgac
     1501 gcaacgcaga gcacaaaatc cgtcgaaccc gctgcccgag acagcctcat ctgcaccaaa
     1561 gcggccaacg ccggccaggg agaggcgatc agaagcgtgc atcccaaggc atacagactt
     1621 ggcacgaagc gatccgacac ctcgccgatt tcattttgag actgcttttg cagaaacgat
     1681 cgggatttct gtcgtattcg aaaaagtgcc aacacgcaca ccagacccaa caacaccatc
     1741 tccggctcgt ccatgaagtc agagcctatt gtctccatcg tctccgccca gtgcctcggc
     1801 cgcaaaagcc agacagaggc gtccactatc tcagtcagat ttcccaagcg cagcgtgggc
     1861 gcacttcgaa cccaaagcac ctgccccttg atgaattcga caaaggctcg agtctctgcc
     1921 gccaggagtc gttccgcggt gtccaaatca accagggcgg caaagtatcg gtcatgttcc
     1981 gtgatcaggg agtcaagcaa tgttctgttg gtttgaacca ggtctcgaat ggcttcttcg
     2041 agtgcacgga tttgcttctc ggtcgttgca gagtccaccc ccgcaacgag tgcttttacc
     2101 tctgtgtcaa tgtcgctgag ttccagacgt tgatcctgca actcgatcag acgcagctgg
     2161 g
//
LOCUS       DAOQUC010000699         2161 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_64041_length_2161_cov_2.652896, whole genome shotgun sequence.
ACCESSION   DAOQUC010000699 DAOQUC010000000
VERSION     DAOQUC010000699.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64041_length_2161_cov_2.652896"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..873
                     /locus_tag="PKH07_11615"
     CDS             <1..873
                     /locus_tag="PKH07_11615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35634.1"
                     /translation="GVRTFLISAEQGSEADRVGDGVKVAKGDDGSLLIDNGRLLLENH
                     STGAIFDRVSLDGIELGSFKALIHQRCGDDLWINADHVEEIIIREGSVRGVFEFVLSY
                     SGQTEHLLRRHDADQFGLLPHRILRPKAFRTAYQVEVLPNQSWFTAKFLWLENTDTEP
                     LDLQEYFYYALSRISGVGTDDEVSQGFTTPNPYCTVASWFDPVQKMHYGVAVPPRSGL
                     TLYFWKDQDGGQHPDLRRSVDLVLAPGKRYSERATPIQVFGLKDDGDKARLSDLWKKM
                     SASHDVTIRAFQDE"
     gene            915..1442
                     /locus_tag="PKH07_11620"
     CDS             915..1442
                     /locus_tag="PKH07_11620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35635.1"
                     /translation="MPSSSRSGTYVGHAPQKPIKAKRKWSVAARNSQATWREVFSNLS
                     PWGYTKLLASLVLLAAVLSWGVGRWAYLNGLTLHSEEMETQTKEYFASRNESLENEIV
                     RLDQKYDLSVLQRRKARKIYSRYYVKHSDEWAKMMISQDTSESDRFWASLMEQCDQEL
                     QTILSKKQMLKGRPR"
     gene            1439..>2161
                     /locus_tag="PKH07_11625"
     CDS             1439..>2161
                     /locus_tag="PKH07_11625"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNT35636.1"
                     /translation="MRLSEGVARTRRSLCLLALHFSAASVAVAERPLSDFRLPQIPWY
                     LNQVFLYPMLIVALVIAVVLRTHYWRKSQAEKEPENAADMELQIPASEGMTGENSSVE
                     ESNDCVGEGDKSGFTLVELIVVVAIIGLLAAIGVSNYMMATIRAKVAACQNNMRVVEG
                     AIMQYRNDFGKYPPFYRPDGSEGFNAWRVIVPMTKRLSVLTTPISYIRTVPRDPFPVT
                     QCSDHTSILFFDTFDYADSDSLA"
BASE COUNT          497 a          534 c          602 g          528 t
ORIGIN      
        1 ggcgttcgga ctttcctgat cagtgctgag cagggctcag aggcagaccg ggtcggcgac
       61 ggagtcaaag tcgccaaggg cgatgacgga agcctgctga ttgacaatgg acgcctgctt
      121 ctggagaacc actcgaccgg tgccatcttt gatcgcgtga gtcttgatgg cattgagttg
      181 ggctctttca aggcattgat ccatcaacgc tgtggcgacg acctctggat caacgctgac
      241 catgttgaag aaatcatcat ccgtgaaggt tcggttcgtg gcgttttcga gtttgttctt
      301 tcctattccg gacagacgga gcatcttctg agacgtcatg atgcggatca gttcggtctg
      361 cttccgcaca gaattctccg acccaaggct tttcgaacgg cctatcaggt tgaggttttg
      421 ccgaaccaga gttggtttac cgccaagttc ctctggcttg agaatacaga taccgagcct
      481 cttgatcttc aggagtattt ctactatgcc ctttcaagaa tctcaggtgt tggcactgat
      541 gatgaggtgt cacagggatt cacgacacct aacccttact gcacggttgc gtcctggttt
      601 gatccggtac agaagatgca ctatggcgtt gcagtgcctc ctcgcagtgg cctcaccctt
      661 tatttctgga aggaccagga tggtggacaa catcccgact tgcgccgcag tgtcgatctt
      721 gtgcttgcgc cgggaaagcg ctattcggaa cgagcgacgc cgattcaggt ctttggtctg
      781 aaagacgatg gagacaaggc aaggctgtcc gatctgtgga aaaagatgtc cgcctctcat
      841 gatgtgacga tccgagcatt tcaagacgag tagtgtttgt gtgtgtcttc cgacaacagt
      901 gggagtattg agcaatgccc agttcatcgc gaagtggaac ctatgtcgga catgctcctc
      961 agaagccaat caaagccaaa cggaagtggt ctgtggctgc tcgaaactct caggcaacct
     1021 ggcgcgaagt cttctcgaac ctttccccat ggggatacac caaactgctg gccagccttg
     1081 ttcttctggc cgcagtgttg agttggggag taggacgctg ggcatatctc aatgggttga
     1141 cattgcacag tgaggagatg gagacacaga cgaaagagta ttttgcttct cggaacgaat
     1201 cactcgagaa cgaaatcgtc cggctggacc agaagtacga tctcagcgtg ctgcagcgaa
     1261 ggaaggcgag gaagatttac tccaggtatt acgtcaaaca ttccgatgaa tgggcaaaga
     1321 tgatgatcag tcaggatacc tcagaaagtg accgattttg ggcgagtctg atggagcagt
     1381 gtgatcagga actccagacc attctgagca agaagcagat gctgaagggg agaccacgat
     1441 gagactctct gagggtgtgg cgagaactcg gcgttctctt tgcctgcttg cgctccactt
     1501 ttctgccgca tccgtagcag tggcggagcg cccactcagt gatttcaggc tccctcagat
     1561 accgtggtat ctgaaccaag tgttcttgta tccgatgctc attgtggctt tggtgatcgc
     1621 tgttgtcctt cgaacacatt actggcgcaa atctcaggcg gagaaagagc cagagaatgc
     1681 agccgatatg gagctacaga tcccggcgtc ggaaggcatg actggtgaaa attcctctgt
     1741 cgaggaaagc aacgactgtg tgggagaagg cgacaaatcc ggattcacgc ttgttgagtt
     1801 gatcgttgtt gtggcaatca tcggactcct tgcggcgatc ggcgtttcca attacatgat
     1861 ggcgactatt cgcgccaagg tcgctgcctg ccagaacaat atgcgtgtcg ttgagggtgc
     1921 catcatgcaa taccggaacg attttgggaa gtacccaccc ttctaccgcc ctgacggaag
     1981 tgagggcttc aatgcatggc gcgtcattgt tccgatgacc aaaagactca gcgttctcac
     2041 gacgccgatc tcctacatcc gaacagtacc tcgggatccc tttccagtaa cccaatgtag
     2101 cgaccatacc agcatcttgt ttttcgatac atttgactat gcggactcgg atagtctggc
     2161 t
//
LOCUS       DAOQUC010000700         2160 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_64064_length_2160_cov_5.058907, whole genome shotgun sequence.
ACCESSION   DAOQUC010000700 DAOQUC010000000
VERSION     DAOQUC010000700.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64064_length_2160_cov_5.058907"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2160
                     /locus_tag="PKH07_11630"
     CDS             <1..>2160
                     /locus_tag="PKH07_11630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35637.1"
                     /translation="IVAGGGAYASNSIAQQTNDLGAYAYKTLKRRGYEGEDILYLSAF
                     GPTDPLDSTKPFRDADGDGLNDVDGWSTLSNLSQIQTFAQGAGRLMVILIDHGFVTNS
                     GCYFQMNQNQVLPATTLDTWLDSLQSAYGVDITLVVDACYSGGFVEACKGGVGGHKRI
                     VISSTTANNVAVFLPPPDMTSFLHTFLGSAYMGNSMGEAWRAGKQFFEEFPVAGQIPQ
                     LDDDSGTTTAADNEFFGASWAYGVQSTQDVNDFFPVFDTWTSDGVVAPGTPVTLWART
                     LSGQNPLGVVAVVRPPAPSTISGDAVTNLPHVALVNSSTDTRLWYATTTDTFTSTGQH
                     VISFTARMPYERRSNPVYSRITVSAGTDTDTTPIRAILAVDNTSDPDLSTAFSKLGPY
                     AYSVYLDRFQDSGGVHHPEWIEYRINPFIHAESDGYPSSSAVLNAIDAVGSDIGRLYV
                     HLIGDSFGSGTIEMASGDDLTASDLDAKLDAYQVRSVTGTVILVVDCPYSGSFLSTCK
                     ATGAQKRVLMTSGRSTDSALFSKSGQAVSFSQKILASAYQGNHLRNGFNSGLNFLTTF
                     LKGGIEPQLDDNGDGVYNSKNDGTFAQTLFLGRRYAYAGGEASDLPFVLNVVPAKVHA
                     PGGSAVGFSADLMEGVEPSRVFAQVVASGVGSVTQPLTTLPEFDFTRDGAEEWSWSGD
                     VTVPAADGSYTVAVYASYPDGATQEKISNPKFATILH"
BASE COUNT          472 a          557 c          639 g          492 t
ORIGIN      
        1 tcatcgttgc cggtggtggt gcgtatgcaa gcaacagcat tgcgcaacag acgaacgatc
       61 tcggtgccta tgcctacaaa acactcaaga ggcgtgggta tgaaggagaa gacatcctct
      121 atctgagcgc ttttggtccg accgatcctt tggattccac gaagcctttc cgcgatgccg
      181 atggggacgg cctgaatgac gtggatggct ggtcaacatt gagcaatctg tcccagattc
      241 agacgttcgc gcaaggggcg ggaaggttga tggtgatcct gatcgatcat ggtttcgtga
      301 ccaacagtgg ctgctatttc cagatgaacc agaatcaggt gctgccagcc acgactctgg
      361 acacctggtt ggacagcctc cagagtgcct atggagtgga catcacgctg gtggtggatg
      421 cgtgctactc aggtgggttt gtggaagcct gcaagggagg tgtgggagga cacaagcgga
      481 ttgtcatttc ctcgacgacg gcaaacaatg tggcggtgtt cctgccgccg ccggacatga
      541 catcgttcct tcacacattc ctcggctctg cctacatggg caactcgatg ggagaagcgt
      601 ggcgagcggg gaaacagttc tttgaggagt tccctgttgc cggccagatc cctcagttgg
      661 atgatgattc cggcacaaca accgcagcgg acaatgagtt tttcggagcc agctgggctt
      721 atggagttca gtccacgcaa gacgtgaacg acttcttccc ggtattcgac acctggacaa
      781 gcgacggagt ggtcgctccg ggaacacccg tcacgctctg ggcaagaaca ctctcaggac
      841 agaatccgct tggggtagtt gccgtggttc gtccaccagc accaagcacc atttccggag
      901 acgccgtgac gaatcttccc catgttgcgc tggtcaacag cagcacagac acacggctat
      961 ggtatgccac gacaacagat acattcactt cgacgggaca acatgtcatt tctttcaccg
     1021 caagaatgcc ctacgaacgc cgctcgaatc ctgtctacag ccgcattacc gtgtcggcag
     1081 ggacggacac cgacacaacc ccgattcgag ccatcctggc agtggacaat acctctgacc
     1141 ctgatctctc tacagcattc agcaagttgg ggccgtatgc gtacagtgtt tatctggacc
     1201 gttttcaaga ttcgggtgga gtccatcatc ctgaatggat tgagtatcgc atcaacccgt
     1261 tcatccacgc cgagagcgac ggctatccga gttcttcggc agtgttgaac gccattgatg
     1321 cggttggatc agatatcggc agactatatg tgcatctgat tggcgacagt tttgggtcag
     1381 gcaccatcga gatggcctct ggagatgatc taacagcgtc ggatctggat gcgaaactgg
     1441 acgcctatca agtgcgttct gtgacgggaa ccgtcattct tgtggtggac tgcccgtatt
     1501 cgggttcgtt cttgtccacc tgcaaggcga cgggagcaca aaaacgggta ctgatgacat
     1561 caggtcgcag cacggactcg gcactgttct ccaagtcggg gcaggcggtg agtttcagcc
     1621 agaagattct ggcgtctgca tatcaaggga atcatctccg caatggattc aattcaggac
     1681 tgaacttctt gacaacgttt ctgaagggcg gcattgagcc acagttggat gacaatggag
     1741 atggcgtcta caacagtaag aacgatggta cttttgcaca gacgcttttc ttggggagaa
     1801 ggtacgccta tgcgggcggc gaggcgtccg atctcccgtt tgtgctgaac gtggttcctg
     1861 caaaagtcca tgcacccggg ggcagtgccg ttggattcag cgctgatctg atggagggtg
     1921 tggagccttc ccgagtcttt gcgcaggtgg tggcttcggg agtgggttcg gttacccagc
     1981 cactgacgac gcttccggaa tttgacttca cacgggatgg agctgaggaa tggtcgtggt
     2041 caggggatgt gactgtgcct gcggcggacg gttcctacac ggttgccgtg tacgcttcct
     2101 atcctgacgg agcgacgcag gagaagatca gcaatccgaa gtttgcgacg attttgcatc
//
LOCUS       DAOQUC010000701         2159 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_64132_length_2159_cov_2.738593, whole genome shotgun sequence.
ACCESSION   DAOQUC010000701 DAOQUC010000000
VERSION     DAOQUC010000701.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64132_length_2159_cov_2.738593"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..328
                     /locus_tag="PKH07_11635"
     CDS             <1..328
                     /locus_tag="PKH07_11635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35638.1"
                     /translation="MRRVVGQDLKRLAPHVDVFSPMVYHKLCKRSPEWVGEVARYVSE
                     QTGKTVWPITQAFNEPTEMSDEEFAQSVRSGLQEPSAGVILFSFGHMEKEGRFPKVRE
                     AFRSVR"
     gene            338..625
                     /locus_tag="PKH07_11640"
     CDS             338..625
                     /locus_tag="PKH07_11640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018467353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HNT35639.1"
                     /translation="MGNETTRAVRVHVKGRVQGVGYRYYAERVASQLGLVGYVKNLRD
                     GRVEAYAEGDQESLAQFLRLLERGPMMSSVEDLDVEWREPTGEYKSFEVTY"
     gene            637..2031
                     /locus_tag="PKH07_11645"
     CDS             637..2031
                     /locus_tag="PKH07_11645"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNT35640.1"
                     /translation="MATVLVVDDEERYLKIYSMNLSDAGYQVHTANSVEKAIQLFEES
                     HPSAVITDLKMPKQTGEDLLNYVSTHSPHTPVIILTAYGDVQSAVAMMRRGAFHYLLK
                     PVEMDELKLFVDQAVRFQQLTLEVEYLREQDKEREGFEGLIGQSPSMMQLFALIDRVA
                     KSDSTVLILGQSGTGKEMVAQAIHRRSSRSSGAFIPTNCIALPRELLESEMFGHVRGS
                     FTGAVGSKVGKFELADKGTLFLDEIGDMDRNLQGKILRVLQDGSIEPVGATTSKKVDV
                     RVLAATNCDLQERVRQGAFREDLFYRLNVVPVVVPPLHDRQEDIPVLVQHFLQKKAQG
                     KTIPKIPSQVIQALMRYSWPGNVRELENLIERFVVLESLDVFRAVGYSLPAAAPPVST
                     QSSVKLMLEQEFSYKDAKAQVVSDFDREFLTEALKKANGNITRAAEIVGMDRKNFSDK
                     LSEIGVDAKKFKNQ"
BASE COUNT          498 a          499 c          655 g          507 t
ORIGIN      
        1 catgcggcgg gttgtcgggc aggacttgaa acggttggcg ccgcatgtgg atgttttttc
       61 gcccatggtg tatcacaagt tgtgtaaacg ctctcctgaa tgggttggag aagtggcgcg
      121 gtatgtgtct gagcagacgg gcaagaccgt ctggccgatc acacaggcgt tcaacgaacc
      181 tacagaaatg agtgatgagg agttcgctca gtcggttcgt tctgggttgc aagagccctc
      241 cgctggtgtg atccttttct cgtttgggca catggaaaag gaaggtcggt ttcccaaggt
      301 tcgggaagcc tttcgttccg tgagatagag tctggcaatg ggtaacgaga cgacaagagc
      361 ggttcgtgtc catgtcaagg gacgagtgca gggcgtcggc tatcgttact atgctgaacg
      421 tgttgcctcg cagcttgggc ttgtggggta tgtgaagaat cttcgggatg gtcgagttga
      481 ggcctatgct gaaggcgacc aggagagtct tgcgcagttt ctgaggcttc tggaacgggg
      541 gccgatgatg tcatctgttg aagatctgga cgttgaatgg cgggaaccga cgggtgaata
      601 caagagtttt gaagtcactt actgacggag tgcatcatgg caacggtgct tgttgtagat
      661 gacgaagagc gatacctgaa aatctactcg atgaaccttt cggatgcggg ctatcaggtt
      721 cataccgcca acagcgtcga aaaggcaata caactttttg aggaaagcca tcccagcgct
      781 gtgatcaccg atctcaagat gccgaagcag accggtgagg acttgctgaa ctatgtttcg
      841 acgcactctc cacacacccc tgtgatcatc cttacggcgt atggcgatgt tcaatctgcc
      901 gttgcaatga tgcgacgtgg agcctttcac tatcttctga aacccgtcga gatggatgag
      961 ctgaaactgt ttgtggatca ggcggtgcga ttccagcagt tgactctcga agtggaatac
     1021 cttcgcgaac aggataagga gcgcgagggt ttcgagggtt tgatcggtca gagtccttcg
     1081 atgatgcagc tttttgcgct tatcgatcgc gttgccaagt ccgacagcac ggtgttgatc
     1141 ctcggacagt cgggcaccgg taaggaaatg gtggcacaag ccatccatcg aaggtcgtca
     1201 agaagctcgg gagcgttcat cccgaccaac tgcatcgcac tgccgcgcga actgcttgag
     1261 agcgagatgt ttggccacgt tcgtggctcg tttaccggtg ccgtcggcag caaagtgggc
     1321 aagtttgagt tggcagacaa gggaacactt ttccttgacg aaatcggaga catggatcga
     1381 aatcttcaag gcaagattct tcgcgtctta caggatggaa gcattgagcc ggtcggagcc
     1441 actacaagca agaaggtgga cgttcgggtg ttggcagcga ccaactgtga tcttcaggaa
     1501 cgcgtgcgac agggtgcatt tcgagaggat ctcttctatc gcctgaatgt cgtgcccgtg
     1561 gtcgttccgc cactccatga ccgacaggaa gacattcctg ttctggtgca acactttctg
     1621 cagaagaagg ctcagggcaa gaccatccca aagattccgt ctcaggtcat ccaggctttg
     1681 atgcggtaca gctggcctgg caatgttcgg gagttggaga acctgatcga gcggttcgtg
     1741 gtgttggaat cgcttgatgt ctttcgagct gttgggtact cgcttcccgc agcagcgccc
     1801 ccagtctcaa cgcagtccag cgtcaaactg atgttggagc aggaattttc gtacaaggac
     1861 gccaaagcgc aagtggtcag cgatttcgac cgtgagtttc tgacagaagc actcaagaag
     1921 gctaacggga acatcactcg agctgctgag attgtgggca tggatcgcaa gaacttcagt
     1981 gacaaactga gcgagatcgg tgtggacgcc aagaagttta agaatcagta gagcaaccca
     2041 caacgggaca tggggaaagg gagaaagaaa tgcttgttcg cactgttctg atgctgttct
     2101 tggctgtctg gttatgttgc tctgccgaca gtcaggcaca agagggaacc tcacatccg
//
LOCUS       DAOQUC010000702         2156 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_64272_length_2156_cov_2.957163, whole genome shotgun sequence.
ACCESSION   DAOQUC010000702 DAOQUC010000000
VERSION     DAOQUC010000702.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2156
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64272_length_2156_cov_2.957163"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(132..1028)
                     /locus_tag="PKH07_11650"
     CDS             complement(132..1028)
                     /locus_tag="PKH07_11650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35641.1"
                     /translation="MSNSQEAFLLIALTGLLWAGVGGFYSRVARKGHRVLLFSLIVSL
                     FVSCFSWAACDWDALLAGAVPKAPIFVPIMLMTGIASQAGMILMMHSMRLGRQAAAWT
                     ISQSAMVVPFLAGVLIWQERMSAGNLLGVVILLVSIVLFGQRVHEEGKPPAKQTWFVL
                     ALLAFAVSGLGATLSTAPSHWHGWQDTARLRVPLLNTAALLAFGTFGVREAMKKMPSK
                     DEWLTAILCALTVFAGQNTFYAGMDRMALVQKVSLTYPLSISLCIMAFAVYTRLHLGE
                     KLGRLGAMGLTLGVIGILLLGI"
     gene            1256..>2156
                     /locus_tag="PKH07_11655"
     CDS             1256..>2156
                     /locus_tag="PKH07_11655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35642.1"
                     /translation="MLRTNARVLMLFLGCSVLLSSGAFAAEVSSIYGIHDASPRPTEY
                     LNRIKAAVGGGWVTATEEIGHDPSNTNGGDFSWFANEGHSVICRLNNGYSNVGTIPLS
                     SQYDNFATRCANYVQNSSGCEIWLIGNETNLAYEWPPNAGHRSYVSPQDYAQCFRKCY
                     NAIKAVRPNHKVICQALAPFAGPFGSGTLWDGTTHDGNPLNWVTYMNQMLTAIKSTGG
                     LDGIAVHINSRGYAYSDVHSTQQINAGGQMLYWSFFVYKDWVNLGTPSDLWHLPFYAT
                     ECNGIYYWKGGHPENPSSHYETDW"
BASE COUNT          538 a          566 c          591 g          461 t
ORIGIN      
        1 ccgatcctgg aaaagtggag tgccgatccg agctgcatcc cctactcagc tggcagctgg
       61 gggcctgctg aggcgaatcg aatccttgag ccgggtcact actggcgaac accttcgtct
      121 tactcagaaa gttagattcc cagaaggagg attccaatca ctccgagagt cagtcccatt
      181 gcgcccaaac gccctaactt ctcacccaga tgcagtctgg tgtacacggc aaaagccatg
      241 atgcagaggc ttatactcag tggataggtg agcgagacct tttgaaccaa agccattcta
      301 tccattcctg catagaatgt attctgtccg gcgaacacag tcagggcaca gagaatggcg
      361 gtgagccatt catccttaga gggcatcttc ttcattgctt ctcggacacc aaatgtgccg
      421 aaggccagga gtgcggcggt attcagaaga ggaacacgca ggcgcgccgt gtcttgccaa
      481 ccatgccagt gtgatggagc ggtggacagg gtggcaccca aaccgctgac agcaaaggcc
      541 agcaacgcca atacgaacca cgtctgcttt gcaggaggtt ttccctcttc atgaactctc
      601 tggccgaaca gaacaatgct aaccagaaga atgacgacac cgaggagatt ccccgcactc
      661 atcctttcct gccagattag gacaccggca aggaagggaa ccaccatcgc cgattggctg
      721 atcgtccaag cggctgcctg tcttcccaga cgcatcgagt gcatcatcag aatcatgcct
      781 gcctgagagg cgataccggt catcagcatg atgggcacaa agataggtgc cttgggcaca
      841 gcaccggcga gcaacgcgtc ccagtcgcag gcagcccagc tgaaacaaga tacgaagagc
      901 gaaacaatga ggctgaaaag aagtacgcga tgtcccttgc gagcgactcg gctgtagaaa
      961 ccgcctacgc cagcccaaag aaggccagtc agcgcgatga gcaagaaggc ttcctgagag
     1021 tttgacaacg aagcgtcctc gattcctgta aacactggag ttcagacaga ggggaatcta
     1081 gtttatgtga attgaggtgt aaagtgaaat gcgattcaac tggcagtttc ctcaactgac
     1141 tgcgaggact cctcccagca ggggttgaag agaagagggt tttaggatac tttgctccat
     1201 acgtggaatg ctggccgaga taccagcgat gctttgcctt ttggaggaat gttccatgct
     1261 gaggacaaac gctcgcgtgc tgatgttgtt cctaggctgc agtgttctgt tgtcgagtgg
     1321 tgcttttgca gcggaagtga gttccatcta tgggatccac gatgcaagtc cgcgcccgac
     1381 ggaatatctg aaccggatca aggctgccgt tggaggtggc tgggtaacgg cgaccgagga
     1441 gatcgggcat gatccctcaa acacaaacgg aggagacttc agctggttcg cgaatgaggg
     1501 tcacagcgtt atctgccgtc tcaataacgg ttattcgaat gttgggacga ttcctctttc
     1561 ttcgcagtat gacaattttg ccacgcgctg cgccaactac gttcagaatt cctccggctg
     1621 cgagatatgg ctcatcggca atgagacaaa tcttgcctat gaatggccgc ccaacgccgg
     1681 acatcgctcc tatgtctcgc ctcaagatta tgcccagtgt tttcggaaat gctacaatgc
     1741 gatcaaggca gtaagaccga atcacaaggt catttgccag gcattggcac cctttgctgg
     1801 cccctttggc agtggcacac tctgggacgg tactacccac gatggcaatc ctttgaactg
     1861 ggtgacgtac atgaaccaga tgctgactgc catcaagtca accggaggtc ttgacggaat
     1921 agcggttcac atcaactcaa ggggctacgc atacagcgat gtgcacagta ctcagcaaat
     1981 caatgcgggt gggcagatgc tctactggag tttctttgtg tacaaggact gggtcaatct
     2041 ggggacgccc agcgatctgt ggcacctccc gttctatgca acggagtgca atgggatcta
     2101 ctactggaag ggcgggcatc ccgagaatcc ttcttcacac tatgagacag actgga
//
LOCUS       DAOQUC010000703         2155 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_64335_length_2155_cov_2.418095, whole genome shotgun sequence.
ACCESSION   DAOQUC010000703 DAOQUC010000000
VERSION     DAOQUC010000703.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2155
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64335_length_2155_cov_2.418095"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2155
                     /locus_tag="PKH07_11660"
     CDS             <1..>2155
                     /locus_tag="PKH07_11660"
                     /inference="COORDINATES: protein motif:HMM:NF017183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HNT35643.1"
                     /translation="VDGTNYQFDVVPDNVIDEKDLAELIRQWPSRGDDPPVVLSSPVE
                     VALVGAPFSYQIAVSDPQGTGAIFRLIEAPQGVTLNADTGLVEWTPNAEQRGRHRISV
                     GITNASGRQTTHYWDVTVGAATVEASGVVNPTQGGVVVVDNPESPLLGARIVIPGGSQ
                     SSLSGVQTADIEIRFIDDEAHFFPDAKPVEIIGLPQMDDAVGLEIPYDASLLSATTVS
                     EQDLDIYHFNPETQEWEPLQRGQIEGAGINIVAKQAGLSTIAAILIPGYSGAKTVVVA
                     TKYLVRLNLQPRKVTLVKGTVDQFCSPSSRARRIIFIHGIASFPGAFMQRVDGRDNPY
                     DFLSSSNVVRNELFSKYDCIATYQYPSGRRIDTNAAVLVEKLKKEYEARRTASDPSMK
                     NNSTFQMDIVAHSMGGLVARWAIEQEGLSHNVRSLVTLGTPHRGFDWLDGLTPFLNLL
                     LDYCGIDLFGIGLVDLKDLLRFSFPGVGQAVKDLDIGGTVFLDTLNELNPPTTVSSAI
                     VNYYGVAGKKGTIPYDPYISISSATYLNLQPGRERIFGDNESQGSDGKNYEHGKLHER
                     AGDPGISAANRTGDQVLAWLAQASLPPPTPASGRWFDGIIGTIATGVTRSDATGDDGH
                     YRKGKPRSFRDNGNGTVTDNNTGLIWVKDPSAAGVGGTYTWSDAITACENLVYAGYSD
                     WRLPTVQELSTLVDAGRFNPAIDPVFVCRDFWYCSS"
BASE COUNT          476 a          538 c          634 g          507 t
ORIGIN      
        1 cggtggatgg tacgaattac cagtttgatg tcgtgccgga caatgttatt gacgaaaaag
       61 acctggcgga gttgatcagg cagtggcctt ctcgtggtga tgatcctcct gtggtcttgt
      121 cctctccagt cgaggtcgct ctggttgggg cacctttctc atatcagatt gcagtcagtg
      181 atccccaggg aacgggcgcg atctttcgtc tcattgaggc accccagggt gtgactctga
      241 acgccgatac cggattggtt gagtggacac cgaacgcaga gcagcgtggt cggcatcgga
      301 tttctgttgg catcaccaac gcaagcggca gacaaaccac gcattactgg gacgttactg
      361 tgggtgctgc gactgtggaa gcatcgggag tcgtgaatcc gacacagggt ggagttgtgg
      421 tcgttgacaa ccccgagagt ccgcttctgg gcgctcggat cgtcattccc ggaggttcgc
      481 agtcttctct atccggggtt cagaccgccg atatagagat cagattcatt gatgacgagg
      541 cacacttttt ccccgatgcc aagccggtgg agatcattgg gcttcctcag atggatgatg
      601 cggtgggctt ggagattcca tacgatgcct cccttttgtc cgcaacgacg gtttccgagc
      661 aggacttgga catctaccat ttcaacccgg agacgcagga gtgggagcct ctgcagagag
      721 gacagatcga aggggctggg atcaatatcg tggcgaaaca ggcagggctt tcgacgatcg
      781 ctgcaatcct cattcctggc tactctggcg caaagactgt tgtggttgca accaagtacc
      841 ttgttcgcct taacctgcaa cctcggaagg tgactctggt caagggaacc gtagatcagt
      901 tttgcagtcc gtcaagcagg gcacgaagga ttatcttcat tcacggcatt gcgtcttttc
      961 ccggcgcttt catgcagaga gtggacggga gagacaaccc atacgatttc ttgtcttcct
     1021 ccaatgtggt tcggaatgaa cttttctcga aatatgattg catcgccacg tatcaatatc
     1081 caagtggaag aaggatagac acaaacgccg cggttcttgt tgagaaactc aagaaggagt
     1141 acgaggcaag gcgtactgca tccgatccgt ccatgaagaa caacagtact tttcagatgg
     1201 acattgttgc gcacagcatg ggtggcttgg tggcgcgttg ggcaatcgag caagagggac
     1261 tctcgcacaa tgtcagaagt ctcgtgacgc tggggactcc gcatcgaggt tttgattggc
     1321 tcgacggcct gacacccttt ctgaacttac tcctggatta ttgtggcatc gatctgttcg
     1381 gaattggact agttgatctc aaggacttac tgagattcag ctttcccgga gtgggtcagg
     1441 cggtgaagga tcttgatatt gggggaacag tgttcctgga cactttgaac gagctgaacc
     1501 ctcccacaac ggttagtagt gcgattgtca actactacgg tgtagcgggc aagaagggaa
     1561 caatccccta tgacccctac atctccatca gttcagccac atatctgaat cttcaaccgg
     1621 ggcgtgaacg catctttggg gacaatgaat cacagggcag cgacggcaag aactatgagc
     1681 acgggaagtt gcatgagcga gcgggggatc cggggatatc tgcagcaaat cggactgggg
     1741 atcaggttct ggcgtggctg gcacaagcaa gtctgccacc gcccacgccg gcctccggtc
     1801 gctggtttga cggtatcatc ggtacaatcg ccacgggtgt tacccgatcc gatgcgactg
     1861 gcgacgatgg ccactacaga aaaggcaagc cgcggtcttt tagagacaat ggcaacggta
     1921 cggtgaccga caacaacacc ggtttgatct gggtgaagga tccctctgcg gcaggtgtag
     1981 gtggtaccta tacctggagc gacgcgatca cggcttgtga gaaccttgtg tacgctggct
     2041 actccgactg gcggctgccg acagtgcagg aactctcgac gcttgtggat gccggacgct
     2101 tcaatcctgc cattgaccct gtgttcgtgt gccgggattt ctggtattgt tcgtc
//
LOCUS       DAOQUC010000704         2149 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_64607_length_2149_cov_2.499045, whole genome shotgun sequence.
ACCESSION   DAOQUC010000704 DAOQUC010000000
VERSION     DAOQUC010000704.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2149
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64607_length_2149_cov_2.499045"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..854)
                     /locus_tag="PKH07_11665"
     CDS             complement(<1..854)
                     /locus_tag="PKH07_11665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35644.1"
                     /translation="MKRFLIFSLLLVLVPNFATAKNPILVDKGGAGDFTTIQQAIESW
                     CPSGTNYAENEPFVIEVSPSAPYIETMSLNDGNTDLTAAPGRGDIKGDIIIRSSVSSV
                     YADVRLNANGEETGAGLRDGLWIYQQTADVTFENIIFSPEVTGTIDDDLVKIDKNADS
                     AANTITFKNCIFTDTKDAGGPLTTSRADAQRSSNVTYVGKLAANDNLVKIWNDNAEFI
                     IVNFDRSIISTAKCNGIVLGGIAGPAVGQPTGPYELNAHECVFSYVGTTSPDSAIRIV
                     DSGDVVPT"
     gene            complement(1153..>2149)
                     /locus_tag="PKH07_11670"
     CDS             complement(1153..>2149)
                     /locus_tag="PKH07_11670"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNT35645.1"
                     /translation="VGKWHLGTDPEYHPQQRGFDEFFGFLGGAHSYSDSVLGVASNAV
                     MRGTEKVDVEYLTDDFSREAVSFVERHSDHPFFLYLPYNAVHTPMHATAKYLERFGSI
                     ADQKRRNYAAMLSAMDDGIGSVLRTLRDKNLEQNTLIFFLSDNGGPIRGNASCNRPLR
                     GEKGQMYEGGIHVPFMMQWKGTLPKGIIYDQPVISLDIHATACSAASVQIREDMKLDG
                     VNLLPYITGKRSDAPHECLFWRSRPNNAIRMGDWKLVTQNNEPTALYNLASDIGETTD
                     LASQNPDRFKQLKARFEEWNSELVDPLWKGKQRKQPATVEPPVRAARPKRKKRQQ"
BASE COUNT          495 a          587 c          549 g          518 t
ORIGIN      
        1 tatgttggaa caacgtcgcc cgagtcaaca atgcgaatcg cggagtcggg cgacgttgtt
       61 ccaacatagg agaagacgca ttcatgagcg ttgagttcat agggacctgt tggctgcccc
      121 acggcaggcc cggcgatgcc accgagcaca ataccgttgc attttgcggt cgagataatg
      181 ctgcgatcaa aattgacgat gatgaactca gcgttgtcgt tccatatctt caccaagttg
      241 tcatttgcag ccagcttccc cacataagta acattcgaag aacgctgagc gtcagcgcgg
      301 ctcgtcgtga gaggcccccc tgcgtctttt gtatcggtga aaatacagtt tttgaaggtg
      361 atggtattgg ctgcactgtc ggcgttcttg tctatcttca caaggtcgtc atcaatggtg
      421 ccagtcacct ctggcgagaa gatgatattc tcaaaagtaa catcggccgt ctgctggtag
      481 atccacagtc cgtcgcgaag tccagcaccg gtttcctcgc cattggcatt gagtcggacg
      541 tccgcgtaca cactcgacac ggaagagcga atgatgatat ctcctttgat atcgccacgc
      601 ccgggagcgg cggtcagatc tgtattgccg tcattgaggc tcatggtctc aatgtatggc
      661 gcggacgggc tgacctcgat aacaaacggc tcattttcgg catagttggt gcctgaagga
      721 caccagctct caatggcttg ctggatcgtt gtgaaatccc cagccccgcc cttgtctacc
      781 aggatgggat tcttggcagt tgcgaaattc ggtaccaaga ccaacaataa ggaaaaaata
      841 agaaaccttt tcatttcagc ctcctcacgt ctccggccta tcaagccgga gaccacccag
      901 aacaaaccgc ccaaaaagta ccgttcacga aaagtctctt cgaacaccct cctttccagt
      961 ctcgcgggga cgagcccact tatgtgctgt ggtcaggcct gtcaacaccg ctctcacaaa
     1021 cgcaaacacg ccctcatcac tcctggttgc tgatgcactt gatccaacct caggtcccga
     1081 tcgccaaagt gagtaagagt ataatactcc tgattccaac agaggtacaa tcgatttccg
     1141 tgttcgattc gatcactgtt gtcgtttctt ccgtttgggt cgggcggctc tcacaggcgg
     1201 ctcgacagta gcgggctgtt ttctttgctt gcctttccag agcgggtcta ccagctcaga
     1261 attccactcc tcaaatcgag ccttcaactg tttgaaccgg tcgggattct gcgacgcaag
     1321 gtctgttgtc tcaccaatat cactcgcaag gttgtagagt gcggtgggtt cgttattctg
     1381 tgtgacgagt ttccagtctc ccattcgaat ggcattgttt ggcctggagc gccagaagag
     1441 acactcatga ggtgcgtcgg aacgcttacc ggtgatatat ggaagcagat tgacaccgtc
     1501 cagtttcatg tcctcgcgta tctgaaccga tgcggcagag caggccgttg cgtgaatgtc
     1561 caaggagatc actggctggt catagatgat tcctttggga agcgttcctt tccactgcat
     1621 cataaatgga acatgaatcc cgccttcata catctgcccc ttttccccgc gcagaggtct
     1681 gttgcaggaa gcgttgcccc gaatcggtcc gccattgtcg ctgaggaaga agatcaacgt
     1741 attctgttcc aggttcttgt cacgaagcgt ccggagcaca cttccaatgc catcatccat
     1801 cgccgagagc atggcagcat agttccggcg cttttgatcc gcgatcgaac cgaaccgttc
     1861 caggtatttc gcagtggcat gcatgggcgt gtgaacggcg ttgtaaggga gatacaggaa
     1921 gaagggatga tccgagtgcc gttcgacgaa agagactgct tcccgtgaga aatcgtcggt
     1981 cagatactcc acgtccacct tctccgttcc gcgcataacc gcattgctcg caacaccaag
     2041 gactgagtca gaataggagt gtgcaccacc gagaaaacca aagaactcat caaatccccg
     2101 ctgctgggga tggtactctg ggtcagtgcc aagatgccac tttccgaca
//
LOCUS       DAOQUC010000705         2148 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_64644_length_2148_cov_2.887243, whole genome shotgun sequence.
ACCESSION   DAOQUC010000705 DAOQUC010000000
VERSION     DAOQUC010000705.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2148
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64644_length_2148_cov_2.887243"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2148)
                     /locus_tag="PKH07_11675"
     CDS             complement(<1..>2148)
                     /locus_tag="PKH07_11675"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNT35646.1"
                     /translation="DVALLTAIGRQVGNAFEAALLNEELQAKEETGRLFGKQLLSLVE
                     VTNELTFQESLEDLCRKAVELGRTKLGFERVGLWLTDAENPEVFHGTFGTDLNGKTRD
                     ERHCQIGVWQDDTLARFRGANPSVPFVEHDVPICDFTLTPVTRGSIGRSLMWDGKENI
                     GVLSSDTAQTGRRITQGESEIFLLYANVLGHLISRERVREALRVQHNRLAVLNAVGQV
                     LTESLNAQQMCDGLLEIFCQQMACDCLLVGKYITESDRIKTIALYDMRDGKIERFPYS
                     DRAFVPEGPCQQALQQAQPVVLFRTSAAQTIPGLYAVGDEEKLSASLMFVRLVVGDRP
                     VGVLSVQSYALNAYTDEDAQLFAAIAQRVAPALEAILLTEQLREREESERIFSQHLVQ
                     LVEVTNELTAEVGFDALCRSAVELGRDRLGFDRVGIWMLERGEAPSIRGTYGTDPQGR
                     TRDERDLVYPIDEATMFPESDLLESGAYMQNLAPLKDMGAVIRDRGTRAWAWMWDGET
                     NVGYVSIDNALSGAPISESQCRTLCLYAATLGHLLGRERDREQIRAQNERLAVLNEVA
                     QLIAGSRNINQMCQRLVEVLSRLMPCEAFVVSRCLPDADQILTLCLYDTLDNTLQPVA
                     PSGVPYKPQGPLQKALKRRQVELFLRDEEISDNQGLTTFGTGRRSASVLFAGLFVGER
                     SVGVISVQSYTKHAYSDHHIDLFGAIAERVAPV"
BASE COUNT          496 a          626 c          555 g          471 t
ORIGIN      
        1 aagactggcg cgacacgttc tgcaatcgca ccaaagagat cgatgtgatg atcggagtag
       61 gcgtgcttcg tgtagctctg aacggaaatc actccaacag atcgctctcc aacaaagagt
      121 ccggcaaaca gaacagacgc cgatcgtctg cccgtcccaa aagttgtcag accttgattg
      181 tcagaaatct cctcatctcg gagaaaaagc tccacctgcc gacgcttgag tgctttctga
      241 agtggtccct gcggtttgta gggaactcca gaaggagcca ctggctgcaa agtattgtcc
      301 agcgtatcat agagacagag cgtcagaatc tgatcggcat caggcaggca tctggagaca
      361 acaaaagcct cgcagggcat gagccgactc aggacttcga ccaggcgctg acacatctgg
      421 ttgatattgc ggcttcctgc aataagttgt gccacttcgt tgagaacggc cagccgttca
      481 ttctgtgccc gaatctgttc cctgtcacgt tcacgaccga gcagatgtcc cagagttgcg
      541 gcgtagagac acagagtcct gcactgactt tcggagatgg gagcaccact gagtgcgtta
      601 tcaatagaca catagccaac gttggtctcc ccgtcccaca tccatgccca ggctcgcgtc
      661 ccacgatccc gaatcacggc acccatgtct ttcaaaggtg ccagattctg catataggct
      721 ccggactcaa ggagatcgct ttcgggaaac atcgtggctt cgtctatggg gtagacgaga
      781 tccctttcgt ctctcgtcct tccctgggga tcggtaccat aggttcccct gatcgaagga
      841 gcctctcccc tctccagcat ccaaatgccc acgcggtcaa aacccaggcg atctcgaccc
      901 agttctacgg cactccggca tagggcatca aaacccacct cggcagtcag ttcgttggtg
      961 acctcaacaa gttgaaccaa gtgctgactg aagatgcgct cggactcttc ccgctccctc
     1021 aactgctctg tcagcagaat tgcctccaat gcgggggcaa cacgctgtgc tatcgcggca
     1081 aacagctggg catcctcgtc ggtgtacgcg ttgagtgcgt aactctgcac cgataggacg
     1141 cccaccggtc ggtcccccac aacgaggcga acgaacatca gcgaagcaga gagtttttcc
     1201 tcgtcgccta cggcgtaaag acccggaatt gtctgcgcgg cactggtacg aaacaagaca
     1261 acaggttgag cctgctgcag ggcttgttgg cagggcccct caggaacaaa agccctgtcc
     1321 gagtaaggga accgctcgat ctttccatcg cgcatgtcat agagagcaat cgttttgata
     1381 cggtcggact cagtaatgta tttcccgacg agaaggcaat cgcaggccat ctgctgacag
     1441 aatatctcga gtagtccgtc acacatctgc tgtgcattga gactttccgt cagcacttgg
     1501 ccgacagcgt tcaagacggc aagacggtta tgctggactc gaagtgcttc gcgaactctc
     1561 tctcgactga tcaggtgtcc gaggacgttg gcgtaaagga gaaaaatctc gctttcgccc
     1621 tgtgtaattc gtctgcctgt ttgggcggtg tccgacgaaa gtactccgat gttttcctta
     1681 ccgtcccaca tcagagatct gccgatgctt cctctcgtga caggagtcaa agtgaagtca
     1741 cagataggta cgtcatgctc gacaaaagga acactggggt tagcccctcg aaaacgagcc
     1801 agcgtatcgt cctgccacac tccgatctga cagtgtcgct catcgcgggt cttgccgttc
     1861 aaatccgtgc caaacgtgcc atgaaacact tcagggtttt cggcgtcagt cagccaaagg
     1921 ccgacgcgct caaagcccag tttcgtgcgg ccgagctcca ccgctttgcg gcagagatcc
     1981 tccagggatt cctgaaaggt cagttcattc gtgacttcga caaggctcag cagctgcttt
     2041 ccgaataggc gtccagtctc ctccttggcc tgcaattctt cgttgagaag tgccgcttca
     2101 aaggcgtttc ccacttgacg gccaatagcg gtcagtaagg caacatcc
//
LOCUS       DAOQUC010000706         2148 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_64649_length_2148_cov_2.584806, whole genome shotgun sequence.
ACCESSION   DAOQUC010000706 DAOQUC010000000
VERSION     DAOQUC010000706.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2148
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64649_length_2148_cov_2.584806"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            261..1304
                     /gene="queA"
                     /locus_tag="PKH07_11680"
     CDS             261..1304
                     /gene="queA"
                     /locus_tag="PKH07_11680"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019850949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HNT35647.1"
                     /translation="MRIEDFDYLLPPDRIAQVPLERREDSRLMVVNRQTGVIQHRCFH
                     DLSSILTQGDLLVLNNTRVFPARLEGLKIPGGALIEMLLLEDLGERRWKVLGRRAKRL
                     RKGTHVEFGEKFGATVEADMGEGLFVVQFSAEGNWDELLERYGQVPLPPYISRESASE
                     QLHREDKQRYQTVYAQHRGSAAAPTAGLHFSESLLEALSQMGVQKTFVTLHIGLDTFQ
                     PVKEEVVERHPIHAERYEISQETSDAVNNAKFDGRRVIAVGTTAVRALESVAGEDGVL
                     EPRHGSTRLFILPGYKFRIIDAMITNFHLPRSTLLMLVSAFMGNTLRRRCYEEAIGGG
                     YRFYSYGDAMLIV"
     gene            complement(1323..1961)
                     /gene="lepB"
                     /locus_tag="PKH07_11685"
     CDS             complement(1323..1961)
                     /gene="lepB"
                     /locus_tag="PKH07_11685"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008712044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HNT35648.1"
                     /translation="MNRLIAFVATVLAKYSFGRRIIQHVKRHPKVYALVQEITVLVVT
                     VLLIRTFIIEPYKIPSGSMIPALGINDRIFVFRLPYWLGKVPDRGDIVVFKVPKTIPN
                     YDPDKPVYIKRVVGLPGETLEIRNGKLFADGQQVTEPEIFDELTYVNQIQGMAKPFTS
                     FQIPDDSILGFGDNTRNSYDSRAWGAIPIRNVKGRAVFRYWPFLPWRVGLIR"
     gene            complement(2023..>2148)
                     /locus_tag="PKH07_11690"
     CDS             complement(2023..>2148)
                     /locus_tag="PKH07_11690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HNT35649.1"
                     /translation="GDNSRNSYDGRAWGGVPLKNVKGKAVLRYWPWAPWRVGLLR"
BASE COUNT          477 a          549 c          589 g          533 t
ORIGIN      
        1 agacaaaacc ctcctctatc cgaaaactca cgaggaagcc atctacccca aacctacctc
       61 gtcctcatcc ctttgtcaag gtcagcggta gtggtttgtc ttgttgctcg gtcagcattt
      121 gagaatgtca agccttacca ctgcgcgttc ctagagacag gcatggagac atcaccacct
      181 tgccctgaaa aggcttcatc ccgggtattt gctctcgcag tatgtgtggg atttcgctat
      241 tgtctcatct gaggctagac atgcgaatag aggattttga ctacctgttg ccaccggatc
      301 gtatcgctca agttccgctt gagaggcgtg aggattcgcg cttgatggtt gtcaacaggc
      361 agaccggggt cattcagcac aggtgtttcc atgacctttc gagcattctg acgcagggcg
      421 atcttctggt tctcaataat acccgggtgt ttcccgcgcg gcttgaagga ttgaagattc
      481 caggaggggc gttgattgag atgctcttgc ttgaggattt gggggagcgc cgctggaagg
      541 ttttggggcg tcgagcgaag agacttcgca aagggactca cgttgagttt ggggagaagt
      601 tcggcgccac tgtggaagcg gacatgggcg aaggtctgtt tgtggttcag ttcagtgctg
      661 aaggcaactg ggatgaactg ctggaacggt acggacaggt tccgctgccc ccgtatattt
      721 cccgcgagag cgcctcggaa cagttacatc gcgaggacaa gcagaggtat cagactgtct
      781 acgcacagca tcggggttct gctgccgcgc caacagccgg tctccatttc tccgagtcat
      841 tacttgaagc gctctcgcaa atgggtgttc agaagacctt cgtgacactg catattggcc
      901 tcgacacgtt tcaacccgtg aaggaggaag tcgtggaacg gcacccaatt cacgctgagc
      961 gttatgagat ttcccaggaa acatctgacg cggtgaacaa cgccaagttc gacggacgac
     1021 gtgtcattgc cgttggaaca actgctgtga gggctttgga gagcgttgcg ggagaagatg
     1081 gggttctgga gccacgtcat ggatcaacaa gacttttcat ccttcccggg tacaagtttc
     1141 ggatcattga cgcgatgatc accaatttcc atctgcctcg ttccacactc ttgatgctcg
     1201 tctcggcatt catgggaaac acgcttagaa gacgatgcta tgaagaagcg attggtggcg
     1261 gataccgttt ctattcgtac ggggatgcga tgctgattgt gtgagacggc accgcaattt
     1321 ctctagcgga tcaaaccaac gcgccaaggc aggaatggcc aataacgaaa caccgctctt
     1381 cccttcacat ttctgatagg aattgcgccc cacgccctgc tgtcataact gtttcgtgta
     1441 ttgtcaccaa agccgaggat gctgtcgtca gggatttgaa aacttgtgaa tggttttgcc
     1501 atgccctgga tctggttgac gtatgtcagc tcgtcaaaga tctcgggctc tgtgacctgt
     1561 tggccgtcgg cgaataactt gccgttgcga atttcgagtg tctctccagg cagtcccacg
     1621 acacgtttga tgtagacagg tttgtccgga tcatagtttg gaatcgtctt gggaaccttg
     1681 aagacaacga tgtctcctct gtcgggaacc ttgccaagcc agtacggaag tcggaatacg
     1741 aagattcgat cgttgatgcc tagagccggg atcatggagc ccgatggaat tttatacggc
     1801 tcaatgatga acgttcgaat caacagcacc gtgaccacca ggacagttat ttcctgaacc
     1861 aaggcgtaga ccttgggatg gcgtttgacg tgttggataa ttctcctgcc gaacgagtac
     1921 tttgcgagta ccgtagcaac aaaagcgata agacgattca cctcaatgca tccttcctat
     1981 gtcctgcccg tctgcaattt tgccgcgggg ggtgcccttc ggtcaccgca gcagtccgac
     2041 tcgccaggga gcccatggcc agtaacgaag aactgctttg cccttcacat tcttcagcgg
     2101 gactcctccc catgccctgc cgtcgtaact gttgcgagag ttgtctcc
//
LOCUS       DAOQUC010000707         2147 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_64674_length_2147_cov_3.581740, whole genome shotgun sequence.
ACCESSION   DAOQUC010000707 DAOQUC010000000
VERSION     DAOQUC010000707.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2147
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64674_length_2147_cov_3.581740"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..835
                     /locus_tag="PKH07_11695"
     CDS             <1..835
                     /locus_tag="PKH07_11695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35650.1"
                     /translation="PLPRGVTHTGRLRMLQAGMGDGLLRVLIESQTKTRRRKVLQDVI
                     IEKVLLKEGTIRYRNQSNSSSPFEMELQRVSALLDDVLLDDKAELRSATLRSLTGFWP
                     ASTEPGLQYAGSFERSGDTYQGHLVGRISAFPLVLVREPIEKELGTVIRGGLVDLDSD
                     VKVHGTLMDSTHTLVINGLDLSMEKSLHRKIKVDLANLVVEILKMLGSEDNIVLENIR
                     LKADLAANTENLWEDLARSALEAVLSRLAERMLHGIREGGEKARERLKQIGESLLDAL
                     E"
     gene            complement(945..1352)
                     /locus_tag="PKH07_11700"
     CDS             complement(945..1352)
                     /locus_tag="PKH07_11700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35651.1"
                     /translation="MGTNAKNQSGHMLAMMLVVIFCSSILLHALLDGQLRSAQLINRD
                     AKSTQAFYLAESAVGHSFSLLRKGSSGAIEATDLGVGHYQSQVETLPDNQGFKIIGKG
                     IVNMPRGEVLTRQVEVVAVKAPEEGWMLTQRVE"
     gene            complement(1373..1879)
                     /locus_tag="PKH07_11705"
     CDS             complement(1373..1879)
                     /locus_tag="PKH07_11705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HNT35652.1"
                     /translation="MRTLHARGFTLVEVLAVASLVGLVSSMILLIASGMSRQTNDMLR
                     IQYAQHETRQIFHDVARDFRNAESTDSAIGDYANSDTTLVLSDVGSSSGVAQPAVVYS
                     QRGNRCWRTEFDSEGAQKASSPVTPENMLARFEKTTPNVLKITVQWVEPRGQSSITRS
                     RSVLVAKR"
     gene            complement(1876..>2147)
                     /locus_tag="PKH07_11710"
     CDS             complement(1876..>2147)
                     /locus_tag="PKH07_11710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35653.1"
                     /translation="EAMTRAAAIASSEMELLINQEGIVLPQEGTVSPPVSVEALDNLP
                     DCEADCHITAGPEEDLAKVTVQVRWKDRAGAHQFELTRLIERKQP"
BASE COUNT          487 a          573 c          572 g          515 t
ORIGIN      
        1 tcccttaccc aggggggtga cacataccgg aaggttaaga atgcttcagg cgggcatggg
       61 agatgggttg ctccgagttc tcatcgaaag tcagacaaag acccgccgca gaaaagtgct
      121 tcaggatgtc atcatcgaaa aagtgttgct gaaagaggga acaattcgct atcgaaacca
      181 atccaattcg agttctcctt ttgagatgga gttgcagcgc gtatcggcac tactggatga
      241 cgtgcttttg gatgacaagg ctgagttgcg aagcgcaact ctccgttctc tgacaggttt
      301 ctggcctgca agcaccgaac caggactgca atacgcagga tcgtttgaga gatctggtga
      361 cacctatcaa ggtcaccttg tcggaaggat atccgcattc cctctcgtcc tggttcgtga
      421 gcccatcgag aaggaacttg gaacggtcat ccgcggtggc cttgtggatt tggacagcga
      481 cgtaaaggtt cacggaacac tcatggattc gacccatacg ctggtcatca atgggcttga
      541 tctctcgatg gagaaaagcc ttcatcgaaa gatcaaggtt gacctggcaa acctggttgt
      601 cgagattctg aagatgctgg gttccgaaga caacatcgtg ttggaaaaca tccgactcaa
      661 agctgacctg gcggcgaaca cggagaatct ctgggaagac cttgctcgaa gtgcgcttga
      721 ggccgtgttg agccgacttg ccgagcgaat gctgcacggt atccgagagg gtggggagaa
      781 ggctcgtgaa agactcaagc aaatcggaga atcgctgctt gatgcgctgg aatgagcgat
      841 ggatctcaga gccttgcctg agtgttccca aaagaaagtc gggttgcgtc tgtgacttca
      901 gccgcaaccc tattcagtaa aggagcctga accatccttg aaagctattc cactcgctgg
      961 gtgagcatcc atccttcttc aggagccttg acggcgacca cttcgacttg acgcgtcagg
     1021 acttcaccac gtggcatatt gactattccc ttgccgatga ttttgaagcc ttggttgtcg
     1081 ggaagcgtct caacctgcga ctggtaatgc ccgactccca gatctgtcgc ttcaatcgct
     1141 ccgctgcttc cttttctcaa gagactaaaa ctatgtccta cggcgctctc ggcaagatag
     1201 aacgcctgag ttgatttggc atcacgattg atcagttgcg cgcttcgcaa ctgcccgtcg
     1261 agaagagcgt ggagcagaat actcgaacag aaaatgacca ccagcatcat cgcgagcatg
     1321 tggccggatt ggttttttgc atttgttccc atgattgatt ctccgcttgt gtttatcgct
     1381 ttgctaccag aaccgatcgt gaacgagtaa tgctcgactg tcctctcggc tccacccact
     1441 gcacggtgat tttcagcacg ttcggggttg tcttttcaaa tcgcgccaac atattctccg
     1501 gcgtgactgg cgatgaagcc ttctgagcac cttctgagtc aaactcagtg cgccagcaac
     1561 ggttcccccg ctgagagtag acgacggcag gttgcgccac tccactgctt gaaccaacgt
     1621 cagacaatac cagtgtcgta tctgaattgg catagtcccc aatcgccgag tctgtgctct
     1681 ctgcgttgcg aaagtcccga gctacatcat ggaatatctg tcgggtttcg tgctgtgcat
     1741 actggatcct gagcatgtcg ttggtctgac ggctcatccc tgaagcgatc agcaggatca
     1801 tggaactgac cagcccgacc agacttgcca ctgccagcac ttcaaccaag gtgaatccgc
     1861 gtgcatgtag tgttctcatg gctgcttcct ttcaatgaga cgagtgagtt caaactggtg
     1921 cgctcccgcg cgatccttcc agcgaacctg caccgtgacc ttcgccagat cctcttcagg
     1981 tccagccgta atgtggcagt cggcttcgca atccgggagg ttgtcaagcg cctcaacgga
     2041 cactggagga ctgacagtgc cttcttgtgg caggacgata ccttcctggt tgatcagaag
     2101 ttccatctcc gagctggcaa tcgccgcagc gcgtgtcatt gcttcca
//
LOCUS       DAOQUC010000708         2143 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_64889_length_2143_cov_3.047414, whole genome shotgun sequence.
ACCESSION   DAOQUC010000708 DAOQUC010000000
VERSION     DAOQUC010000708.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2143
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64889_length_2143_cov_3.047414"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1861)
                     /locus_tag="PKH07_11715"
     CDS             complement(<1..1861)
                     /locus_tag="PKH07_11715"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HNT35654.1"
                     /translation="MKWIVSAVVVIALTVSFAVWTGRPKPLELKVPPSISEGGVAEDL
                     DFERIQDSIRRFSGVESRVTGYPGCQQTLELIQRELTRIGVTDFQVQEFSVPAPIVES
                     SVLIGHTDSGDITIPVHPLWPNLARTCQTGPDGISGSLIDVGAGNDNDLAGKDLRERI
                     ALMDWNSRSEWLNVPEFGGKAVIFRANDRGDGYTARDKFMTIPADFPRFYVTAEFFPT
                     LDELLSAQAKVTLHCQMQWRNVTARNLLVRVCGTKDNSKNINRDEASLIFHAYYDSIS
                     VVPGLAPGAEQACGAATLLELAHYFSKKEFDRPIYLLFTGAHGQALEGMAHFVRTLRD
                     GSKSNWAGEPKDSLIVRMGKPSLFVGLDLSSRSERLGVFCVGRFRAQREDRLRPKFST
                     LGLKLDEYAKSFQTVPDATESLSPFVDCINLTLGRGWWTYFPYQAPFESELPNLSAYP
                     AITLSTINDDRRYVDTPDDTWDRMRPDLFKRQIMPRQGEHVGLANLALALASWSGPFT
                     GGDLSDVWSRLSGRVVWLNQQEDYTPNEPLVAATVFLKTQRGDKYLMGTRGIPCAMTD
                     DNGTFHFDGLTNITANWDFNNCTVEPYGTATSDFISKNQTAYDELMKVRNRGGK"
BASE COUNT          516 a          575 c          574 g          478 t
ORIGIN      
        1 cctttccacc acggttgcga accttcatca actcgtcata ggccgtttga ttcttcgaga
       61 tgaagtcgga ggtcgctgta ccgtagggtt ccacggtgca gttattgaaa tcccagtttg
      121 ccgtgatgtt tgtcaggcca tcaaaatgaa aggtgccatt gtcatcagtc atggcacagg
      181 gaatgcctcg tgttcccatg aggtacttgt cgcctcgttg tgtcttgagg aatacggtag
      241 ctgctacaag tggctcattc ggggtatagt cctcttgctg attcaaccag actacgcgtc
      301 cactgagtcg cgaccagaca tcgctcagat ctccccctgt aaaaggacca ctccatgacg
      361 ccagagcaag agccagattt gccaagccaa cgtgctcacc ctgacgaggc atgatctgtc
      421 gcttgaaaag atcgggacgc atcctgtccc atgtgtcgtc aggtgtgtcc acatagcgtc
      481 ggtcgtcatt gatggtggac agcgtgattg ccggataggc gctcagattg ggcagttcac
      541 tttcaaaggg tgcctgatat gggaagtaag tccaccatcc acgacccaga gtcaaattga
      601 tgcagtccac aaacggagag agtgattcgg tggcgtcagg gactgtctgg aaagacttgg
      661 cgtactcatc caacttgagc ccgagtgtgc tgaacttcgg tctgagccta tcttcccttt
      721 gggcgcgaaa acggccgacg cagaaaacac ccaggcgttc agagcggctg gatagatcca
      781 gaccgacgaa gagactgggc ttgcccattc taacgatgag cgagtctttt ggttcgccag
      841 cccagttgga ttttgaacca tcacgcaagg tccgaacgaa atgcgccatc ccctcaagtg
      901 cctgtccatg ggcgccggtg aacagcaggt agatgggtcg gtcaaattcc tttttggaga
      961 agtagtgggc cagttccagg agggttgcag ctccgcaggc ttgctcggca ccgggagcga
     1021 gtccaggaac gacactgatg ctgtcatagt aggcatggaa gataagactg gcttcatctc
     1081 ggttgatgtt cttggagtta tccttagtac cgcaaacccg aacgagaaga ttgcgcgctg
     1141 tcacattcct ccattgcatc tggcaatgaa gagtgacttt cgcctgagct gaaagaagct
     1201 catcgagcgt ggggaaaaac tcagcagtga cgtagaaccg cgggaagtcg gccgggatgg
     1261 tcatgaactt gtcccgtgcc gtgtagccat ctccgcgatc attggcgcga aagatcaccg
     1321 ctttgccccc aaactcagga acgttgagcc attccgagcg cgaattccag tccatgagag
     1381 cgatccgctc tctgagatcc tttccggcaa ggtcgttgtc attgcctgca cccacatcaa
     1441 tgagggagcc tgagatgccg tcaggaccag tctgacacgt tcgagccaga tttggccata
     1501 gcgggtggac tggaatggta atatctccgc tgtcggtgtg accgatcaga acagacgact
     1561 caacaatcgg tgcgggaacg gaaaactctt gaacttggaa atcggtcaca ccaatacgag
     1621 tcagctctcg ctggataagc tccagagtct gctggcaacc cggatagcct gtgacacgag
     1681 attcaacgcc gctgaatcgg cggattgagt cctggattct ctcaaaatcg agatcctctg
     1741 cgaccccacc ttctgaaatg gatggaggaa ccttcaactc aagaggtttg ggacgccccg
     1801 tccatactgc aaacgagaca gtcagagcga tgacaacaac ggctgatact atccatttca
     1861 tatcatgcag ttccaagcga aaaagttctt tgccagttcc aactgaggtg caggcgctgt
     1921 tccctcattg gtctcctgca tataggccca cacacacaag gtctgtgtgg acgtcgtcaa
     1981 ccccagaagt ggtataccgt cgtcccgaca atcgaaccgt agatgcacca gaggcttccc
     2041 actacgaact cacccagcac caaaccgaca aagaaaggca ttgcctttcg atatgccacg
     2101 gagcctccat atcgggtgat gattgctttg gcaagccatc cga
//
LOCUS       DAOQUC010000709         2143 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_64896_length_2143_cov_2.794061, whole genome shotgun sequence.
ACCESSION   DAOQUC010000709 DAOQUC010000000
VERSION     DAOQUC010000709.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2143
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64896_length_2143_cov_2.794061"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..466)
                     /locus_tag="PKH07_11720"
     CDS             complement(<1..466)
                     /locus_tag="PKH07_11720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase N-terminal domain-containing
                     protein"
                     /protein_id="HNT35655.1"
                     /translation="MNLSHWTQCLLSVCFAMHCSTLTAQELTWRDATSFEIEGRGWSQ
                     TAGPFDRLPDSAREKVPPTAWDLSKDSAGICVRFMTDAGSLDVRWSLTEDALAMPHMP
                     ATGVSGVDLYLRSAKGDWKFVGNGRPHKVEGNSSTFSLSEEQKTLRECLLYLP"
     gene            complement(622..1734)
                     /locus_tag="PKH07_11725"
     CDS             complement(622..1734)
                     /locus_tag="PKH07_11725"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HNT35656.1"
                     /translation="MFCEDYCVARPEDIETPALLVFEDRLDGNIKALIEQVGGGERLA
                     PHVKTHKSAEILRRQMAAGIRSFKCATLSEAEMIARGGADDLLLAYPIAQPLKARRFM
                     ELKARYPKTLFRAMVSSPAHAEILSAAAVESGDNVDVMIDLDVGMHRTGIEPGEAAFE
                     LCLLIHKTPGLRYAGIHAYDGQNNQTDIAERESAAGEALDFVKSLRDRLKSVGLETST
                     IMMGGSICYPFYTREDGIQGSPGTVAYWDGNYMKQMPDMPFRCAALVLTQVVDSNPAR
                     DLVTLDLGSKAICCDQVLANRALFPGYPQVSIVKQNEEHCVLKCNGADLKIGDYLLAI
                     PGHVCTAVVRYPGALLINRVGEVSGYIEHTSRDRLT"
     gene            complement(1840..2063)
                     /locus_tag="PKH07_11730"
                     /pseudo
     CDS             complement(1840..2063)
                     /locus_tag="PKH07_11730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017749610.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
BASE COUNT          495 a          607 c          539 g          502 t
ORIGIN      
        1 gagggagata gagcaagcat tcacgtaggg ttttctgttc ttcagatagc gaaaaagttg
       61 aactgttgcc ctccactttg tgcggtctgc cgtttccgac gaacttccag tcacccttgg
      121 ctgaacgaag atacagatca acaccactga ctcccgttgc tggcatgtgg ggcattgcga
      181 gtgcgtcttc agtaagtgac cagcgcacat cgagtgaccc cgcatcagtc atgaagcgaa
      241 cacagattcc cgcggaatcc ttgctcaaat cccacgccgt tggcgggact ttctctctgg
      301 cggaatcagg aaggcggtcg aaagggcctg cggtttggct ccaccccctg ccctcgatct
      361 cgaatgatgt cgcgtctcgc caggtgagtt cctgggcggt caaagtgctg caatgcatgg
      421 caaagcacac agacaaaagg cactgagtcc agtgagatag attcattggc gttgaacctc
      481 ttttcttgat tggcatggac gaatcgtggc agagggcaca caatgcgtca agtgacagtc
      541 ctcaacgcct cggcgacttc acgccgaaag aaagaggaca ctgggtcgag tgcagcgttg
      601 aagaggcaga tctcactcca ctcaagtgag cctgtctctt gacgtgtgct caatgtatcc
      661 gctgacttca ccaactctgt tgatcagcag agcacccgga tagcgcacta cggcagtgca
      721 gacatgtcca ggaattgcca gaaggtaatc gccgatcttc agatcagctc cgttgcactt
      781 cagcacgcag tgctcttcgt tctgcttgac aatgctgact tggggatatc cggggaagag
      841 ggcacggttg gccaagacct ggtcacagca tatcgctttc gaccccaaat ccagcgtcac
      901 tagatcgcgc gcaggattgg agtcaaccac ttgggtcaga accaacgcgg cacatcggaa
      961 aggcatatct ggcatctgtt tcatatagtt gccatcccaa taggcgacag ttcccggaga
     1021 gccttgaatc ccgtcttcac gagtatagaa cggatagcat atcgagccgc ccatcatgat
     1081 cgtggatgtc tcaagtccaa ctgacttcag cctgtccctc aaactcttca caaagtccaa
     1141 agcctctccc gccgcagatt cacgctcggc aatatcggtc tggttgtttt gtccatcgta
     1201 tgcgtgaatt ccggcatagc gcagtccagg cgtcttgtga atcagcaggc acagttcaaa
     1261 ggccgcctct cccggctcga ttcctgtccg atgcatcccc acatccagat cgatcatgac
     1321 atccacgttg tctcctgatt caaccgccgc tgccgagaga atctctgcat gagcagggga
     1381 agaaaccatc gctcgaaaga gggtcttggg atatctcgct ttgagctcca tgaagcgcct
     1441 tgccttaaga ggttgggcga tcggataggc aagaagaaga tcgtccgcac cgccccgtgc
     1501 gatcatctcc gcttcactca gtgtggcgca cttgaaactc ctgattcccg cagccatttg
     1561 acgccggaga atctcggcgg acttgtgcgt cttgacatgc ggagcgagtc tctctccacc
     1621 accgacctgt tcgatcaacg ctttgatgtt tccgtcgagt ctgtcttcaa acaccaggag
     1681 tgctggcgtt tcgatatcct caggcctcgc aacgcagtag tcttcacaaa acatctcttc
     1741 tccctttcgg actgaatcta tctcttctct caagaggcga attattgcag aaggctcacc
     1801 ccatccggcg acttacgaga ctcgtttctg gcgacaaagt tagatctcat tgagcaattc
     1861 ctgaatcgga acaccaatat ccttggcgat ctggcgaaga aggattggtg acacatcgcg
     1921 ccctgtgtgg aatgggacgg ttgtcgctct tccatcggga tgtctgaact gtctgtggga
     1981 accgattgtc tcacttgcga gaatcccagg cgacaaagaa ctttggcaat ctgatcaggc
     2041 ttgagtacag gaatccttcc catcgttcag gctaccacga cactctgcgt tccaataaac
     2101 tctgactcca ataagggttc accctcctca aggagcattt cca
//
LOCUS       DAOQUC010000710         2139 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_65078_length_2139_cov_2.879559, whole genome shotgun sequence.
ACCESSION   DAOQUC010000710 DAOQUC010000000
VERSION     DAOQUC010000710.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2139
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65078_length_2139_cov_2.879559"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>454
                     /locus_tag="PKH07_11735"
     CDS             <1..>454
                     /locus_tag="PKH07_11735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35657.1"
                     /translation="ETLESISKHTGGVAYDAPPGDYSISLAAVESASADMNLIRESAT
                     IPTLTPSWAFPGTAQETLPAVGSWLKTQPRSNTSALLTVLDQEPLIATMYFGLGRTAA
                     VSLADDQFSWFAQPITATIFANLARLTSSHLRVHKVEIQRAIHGDQVTL"
     assembly_gap    455..464
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <465..887
                     /locus_tag="PKH07_11740"
     CDS             <465..887
                     /locus_tag="PKH07_11740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35658.1"
                     /translation="LWFRFDKKVQTDITSHLTLPDGGVRTLPLVKQHESLWSTSMTME
                     QTGTYRLAVTSAEGEILARDRWIHSCLSEYTALGPNLPELRRIARATGGTLLEEGFLP
                     SEAIASIEQSARPIPQRVDMTDIVLMAAVLLFLVSLFL"
     gene            complement(861..>2139)
                     /locus_tag="PKH07_11745"
     CDS             complement(861..>2139)
                     /locus_tag="PKH07_11745"
                     /inference="COORDINATES: protein motif:HMM:NF016906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HNT35659.1"
                     /translation="WKQGSGTDSSLLAFMMAVFSANCFAQGGVSVISHGAVGDGEYDC
                     TEAFRAAIAKASAEKSYVYVPRGNYAISQTLELDNVGISGEPVGAWPSDVESLPSILL
                     KHSDSPGFRLLAGGSLRGLDITYRPKEKSDGNAAVLISGIGVYIANMRIRYTWDGILT
                     DGINNVGRLNIENVFIVAVRNVGVRVSGTWDVPRLNNIEVWNAAMGRGLEKGVGFHLL
                     KNDLIRVTDCFAFGMGVGFLLEDKMEGSPIEGGTWGVMTGCSTDFCGTGVRVKGSHTV
                     SVTGGTFWQHHRTLEVDGADARVRFTGAEIKSNGAPAIVVNDADHVVVTGCTIRREME
                     NHDVPAVVLRGGSTTLSANHIISGATGVAVYSGVRSAVIGNNTISSRKIPLWVDETLS
                     RSRVSICGNLLLDSESGEAFVPECCSEKETDKE"
BASE COUNT          527 a          634 c          488 g          480 t
ORIGIN      
        1 gagaaacgct ggagtccata tcaaaacaca ctggcggtgt tgcctacgat gccccacccg
       61 gggactattc catctccttg gcggctgttg aatccgcgtc ggcggacatg aatctgatca
      121 gagagagcgc aacgattccg actttgactc cctcctgggc gtttcctggt actgcgcagg
      181 aaactcttcc cgctgtcgga tcgtggctca agactcaacc tcgcagcaac acctccgctt
      241 tgctcacggt actcgatcag gaacccctga tcgccacaat gtatttcgga cttggcagaa
      301 cggccgctgt ctcgctcgcg gatgaccagt tttcatggtt tgcccaaccc attaccgcaa
      361 cgatattcgc caatctggcg cgtctcacaa gttcacattt gcgcgttcac aaggtcgaga
      421 ttcagcgggc tattcatgga gaccaagtca ctctnnnnnn nnnnctctgg tttcggtttg
      481 acaaaaaagt ccaaactgat atcacttctc atctcacgct tcctgacggt ggcgtgcgca
      541 cactgccact cgtcaaacaa catgaatcac tttggtccac ctcaatgacc atggagcaga
      601 cagggactta caggctggca gttacctcag cagagggaga aatacttgcc agagaccgat
      661 ggattcacag ttgtttatcc gagtatacag cactcggccc aaatctgccg gagctgagaa
      721 gaatcgcccg agcgactggc ggaacgcttc ttgaagaggg atttctccca tctgaagcaa
      781 tagccagcat tgagcagtct gcaagaccca ttccccaacg agttgatatg accgacatcg
      841 tgctgatggc cgccgtgctt ctattccttg tcagtctctt tctctgaaca gcactcagga
      901 acaaacgcct cacctgattc gctgtccaga agcaggttgc cgcagataga gactctcgaa
      961 cgagaaagtg tctcatcaac ccagagaggg attttcctgc tgctgatggt gttgttcccg
     1021 atcaccgccg atcgcactcc actgtagaca gcaacgcccg ttgcgcccga aatgatgtga
     1081 ttggcagaga gcgtggtcga gccacctctg agaacaacag cgggaacatc gtggttttcc
     1141 atttcgcgtc tgatggtaca tccggtgacg accacatggt cggcatcgtt gacaacaatg
     1201 gcaggcgctc cattggattt gatctcagca cctgtgaaac gcactctggc atcggcacca
     1261 tcaacctcga gtgtcctgtg gtgttgccag aatgtcccgc ctgtcacgga aacagtatgg
     1321 gagcctttga ctcgaacacc ggtaccgcag aaatctgtcg aacagcccgt cataacaccc
     1381 caagttcccc cttcgatggg agaaccttcc atcttgtctt ccagaagaaa gcctaccccc
     1441 atcccaaatg caaaacagtc tgtcacacga atgagatcgt ttttcagaag atggaaacca
     1501 acgcccttct ccaaacctcg ccccatggca gcattccaga cttcaatgtt gttcaaacgt
     1561 ggaacatccc aagtaccaga aactcgaact ccaacattgc gaacagcgac gatgaacaca
     1621 ttttcaatgt tcaggcgacc cacattgttg atgccatccg taaggatgcc atcccaggta
     1681 tatcgaatcc tcatgttggc aatgtagacg ccaatgccgc tgatcagaac cgcggcgttg
     1741 ccatctgact tttccttagg acgatacgtg atatccagac ccctcagtga accacctgca
     1801 agaagcctga atccgggact gtccgaatgt ttgagaagaa ttgaaggaag tgattcgaca
     1861 tccgaaggcc aggctccaac tggttcccca ctgatcccaa cattgtccag ttccaaagtc
     1921 tgggagattg catagttccc tcttggcaca tagacataac tcttctcggc actcgccttc
     1981 gcaatcgccg cacggaaagc ttcggtacag tcatattcgc cgtcacccac cgccccatga
     2041 ctgatcacac tgaccccacc ctgcgcaaaa caatttgcac tgaacaccgc catcatgaat
     2101 gcaagcagac tcgaatcggt tcccgatccc tgtttccag
//
LOCUS       DAOQUC010000711         2138 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_65109_length_2138_cov_3.632261, whole genome shotgun sequence.
ACCESSION   DAOQUC010000711 DAOQUC010000000
VERSION     DAOQUC010000711.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2138
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65109_length_2138_cov_3.632261"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..732)
                     /locus_tag="PKH07_11750"
     CDS             complement(<1..732)
                     /locus_tag="PKH07_11750"
                     /inference="COORDINATES: protein motif:HMM:NF021445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HNT35660.1"
                     /translation="MMKVTPNMLMNVPPLPEFRELTWDDRNIYRDWCRHFGVDASELT
                     FTNLFAWRRSEPVMISRLDMLLVVLRLDREKRPSLCVPIGEGDLSAVTEQMLSLLQTI
                     SSEDSPVLRRVPEFYASAIAELGYPVEEDRDNFDYVYRVDDLAELPGRKYDGKRNHVR
                     KCLESSPCEYEEINRNNLDDCFSLATEWCNVRQCDIHPPLASEFRAIRETFERFEELG
                     LLGGAIRVHGEIQAFAIGEAINEGTA"
     gene            1373..>2138
                     /locus_tag="PKH07_11755"
     CDS             1373..>2138
                     /locus_tag="PKH07_11755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35661.1"
                     /translation="MIRKKVSSTLYLCCLLLCLFPTRADSLLADPIIVADPELVTPTL
                     AWEKASDATAYERSFYWKLPGGFRTAYAEWGVAIPELANYAIAIWYPAFADSKEGVSR
                     DTPVHLYFNGTLIRDDIYVDQSLYPGQWHHIAVVRLGKGTLTIRMSDQASAGAVLADA
                     VGVVETIGATSTPTRTPTRMPFLTYTPTRTPTRTFTATHTFTSSPTLTPTQTPSRTPS
                     LTPTQTRTPVPTANVQALDMPWSRELAGSDSDMGQAI"
BASE COUNT          511 a          593 c          534 g          500 t
ORIGIN      
        1 tgctgtgcct tcgttgatcg cctcaccgat ggcaaacgcc tgtatctctc cgtgaacgcg
       61 aatcgcccca ccgagcaacc ccagttcctc aaatcgctca aaggtctccc gaatggcgcg
      121 gaactcagag gctagaggag gatgaatgtc acactgtcgc acattgcacc attcggtcgc
      181 cagactaaag cagtcgtcca gattgttacg attgatctcc tcatactcac agggactgct
      241 ttccaggcac ttgcgtacgt ggttgcgctt tccgtcatac ttgcgacctg gtagttccgc
      301 cagatcgtca accctgtaga catagtcgaa attgtcgcga tcttcctcga cgggatagcc
      361 caattcagca atcgcagatg cgtaaaattc cggaacccga cgcaacactg gagaatcctc
      421 ggaagagatc gtttgaagca ggctgagcat ttgctccgtc acggccgaga gatcgccctc
      481 tccaattgga acgcataggg atggacgctt ctccctgtcc aaacgcagaa caaccagtag
      541 catgtccagc ctggatatca tcacaggttc gctgcgacgc catgcaaaca ggtttgtgaa
      601 cgtgagttct gatgcgtcaa caccgaaatg cctgcaccag tctcgataaa tgttccgatc
      661 atcccaagtc agttccctga actccggaag aggtggaaca ttcatgagca tgttgggagt
      721 cactttcatc atctgacctc caagaaagaa actgtctctt ctcgagtctg gtttcccacg
      781 agaatatgat gggcgtaaac tagcatgaaa aacgctacgg ggcacgcaac aattctatgt
      841 ctcaaaataa gccattcaaa tctcattatg ggacatcact ggatggctct ctgtgttttg
      901 cattctcgct gcgatcacag ttcgctgctc tggaagcact tgcagagttc caactgaatt
      961 gtgctcttcc gtgtcaatgg attggcattg cctatgctat ttgatgaagg ttgaagacct
     1021 gaaacgaaca ggagaatgca acaaaagtat acatcacaaa agggaatgcg gtatactagg
     1081 taccgctccc gcaatctgag agcgtttagg ggaacgccct tgcgggggca cgatgttgac
     1141 ccccccttga tgagacccat caaggggggg tgcagggctt tctcaagtgg gttggcaccg
     1201 cagacgagtg ggctgttctc actcctgtct gtgcttgact cgcgaaagag gccgcccatt
     1261 ccgaagtctg gcttgccagg agcagtcttt gactgcttgg agcatcattt gatgcggagt
     1321 ttcaccgatg ctgtcacggt cgggaaactc accgcagaag ggattttgaa agatgatccg
     1381 caagaaagtc tcgtcaaccc tctacctgtg ttgcttgctt ctgtgcctgt ttccaacaag
     1441 ggcagactcg cttctagctg atccgataat tgtcgccgat cccgagttgg tcactccgac
     1501 actggcatgg gagaaggcgt ctgatgcgac tgcctacgag cgttctttct actggaaact
     1561 tcctggagga ttccgaactg cctacgcaga atggggcgtg gcgattccgg agctcgcaaa
     1621 ctacgcgatc gccatctggt atcccgcctt tgcggactcc aaggaaggtg tttcgagaga
     1681 tactccagta catctttact tcaacggaac actgatccga gatgatatct acgtcgatca
     1741 gtctctctat cctggccaat ggcatcacat tgcagttgtc agacttggca agggaactct
     1801 gacgatccgt atgtccgatc aggccagtgc cggagcagtt cttgccgacg ccgttggagt
     1861 tgttgaaacc attggagcga cctctacacc tactcgaaca cccacaagaa tgccttttct
     1921 gacgtacact ccgaccagaa cgccaacaag gacgttcact gcaacccaca cgtttacaag
     1981 ctccccaacg ttgacgccta cacagacacc ctcaagaaca ccctctctga cgcctacgca
     2041 aacgcggact ccggttccga cagcaaacgt gcaggcgctt gatatgcctt ggtcccggga
     2101 acttgctggc tcggactcgg atatgggtca ggcgatcg
//
LOCUS       DAOQUC010000712         2138 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_65122_length_2138_cov_2.801728, whole genome shotgun sequence.
ACCESSION   DAOQUC010000712 DAOQUC010000000
VERSION     DAOQUC010000712.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2138
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65122_length_2138_cov_2.801728"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..823
                     /locus_tag="PKH07_11760"
     CDS             <1..823
                     /locus_tag="PKH07_11760"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HNT35662.1"
                     /translation="ASAQVKSAILLAGLNASGETEIEEPGLSRDHTERMLRDFGVKLD
                     TENNRVRLQGHQPLRGRMVHVPGDFSSAAFFIAAATLVPDSAIALSNVGLNPTRTGLL
                     DALYSMSPGHIEVIPETADLAEESGTIRVQSCPLKSTSVGEELVVRMIDEFPIFAALA
                     SLAKGKTTIRNASELRVKESDRIAVMAQQLRKMGVEVQEFPDGMDVVGSEGLHAATVE
                     SHGDHRIAMSLAVAALRSSGETVIENTDCIATSFPGFVDLMRELGADIEEQECRT"
     gene            811..1548
                     /locus_tag="PKH07_11765"
     CDS             811..1548
                     /locus_tag="PKH07_11765"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNT35663.1"
                     /translation="MSDVTSTGTKERLESEGKPGSQVPECNKAREAFKWTLASRVVYS
                     VSKFFLWLTVKVLFRARYEGTENLPKTGPVLLAPNHASYIDPPLVGAGIPRRITFLAK
                     EELFRGLLGWWIRTVGGKPVFRGKGDVGVFRIAEKAFQNGEVLLLFPEGTRTHDGRLQ
                     PMRDGIGLIVKRTGVPLVPVGVMGTYDAWPRHRAFPCLKKVVVRYGKPLDFSELTSAQ
                     AGREVYREITNKLGEEIARLCQQEKEG"
     gene            1656..>2138
                     /locus_tag="PKH07_11770"
     CDS             1656..>2138
                     /locus_tag="PKH07_11770"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HNT35664.1"
                     /translation="MASVDNMGEERDDINGLENEADNSGLEDFDKLQEFYDGSFKEIR
                     SGQVVKGRVVSVKQDEVIVDIGAKCEGAIALEEFMSDDKDRNVYVGREFEVFIVKRED
                     QEGAPVLSRRRAREIVTRENIKRAYASGEPVECRAIKVVKGGLQVDCGINGFMPFSHT
                     G"
BASE COUNT          511 a          497 c          654 g          476 t
ORIGIN      
        1 cgcgagtgcg caggtgaaat ccgcgatcct gcttgctggc ctgaatgcgt caggtgagac
       61 agaaatcgaa gaacctggac tgtcacgcga tcacacggaa cgcatgctgc gagacttcgg
      121 cgtcaaactc gatactgaga acaacagagt gcgccttcaa ggccaccagc cgctccgtgg
      181 caggatggtt cacgttccgg gggacttctc ttctgcggcc ttcttcattg ccgccgcaac
      241 actggttcca gactcggcaa tcgctctctc gaatgtgggt ctgaacccaa cacgaaccgg
      301 actgcttgac gcgctgtact cgatgtctcc aggtcatatc gaggtcattc ccgaaacggc
      361 cgatcttgcc gaggagtcgg gaacgattcg cgtgcagagt tgcccactga agtcaacttc
      421 agttggggaa gaactggtcg tgcggatgat cgatgagttt ccgattttcg ctgcactggc
      481 atcccttgcc aagggaaaaa ccaccattcg taacgcctca gaactccgtg tcaaggaaag
      541 tgaccgaatc gcggtcatgg cgcagcagtt gcgtaagatg ggagtcgaag ttcaggaatt
      601 ccctgatggc atggatgttg tcggctcaga ggggttgcac gctgccacgg tggaaagcca
      661 cggggatcat cgcatcgcga tgtctcttgc ggtcgcggct ttgagatcca gtggagagac
      721 tgtcatcgag aacaccgact gcattgcaac gtcttttcct ggttttgtgg atttgatgag
      781 agagttggga gctgacatcg aggagcagga atgtcggacg tgacaagtac aggcacaaaa
      841 gaacgattgg aatctgaggg aaaaccggga tcgcaagtgc ccgaatgcaa caaagccaga
      901 gaggcgttca agtggacgtt ggcaagtcgc gtggtctact cggtaagtaa gttctttctt
      961 tggctcacgg tcaaagtcct gttcagagcc cgctacgaag gtacggagaa tctacccaag
     1021 accggccctg tattgctggc tccgaaccac gccagttaca ttgatccccc tctggtaggc
     1081 gcaggaatac ccagacggat caccttcctt gccaaggagg aactgtttcg cgggcttttg
     1141 ggttggtgga ttcgaaccgt ggggggaaaa cccgtttttc gcggtaaagg agatgtcgga
     1201 gtattccgaa ttgcagagaa agcttttcag aatggcgaag tgctgctatt attccctgaa
     1261 ggtacaagga cacatgatgg ccggcttcag ccaatgcgtg atggcattgg acttatcgtg
     1321 aagcgtaccg gggttccttt ggttccagtt ggagttatgg gaacctatga cgcctggccg
     1381 cgtcatcggg cgtttccgtg tttgaagaag gttgttgtgc gttatggaaa accgttggat
     1441 ttctctgagc tgacttcggc tcaggcgggt cgtgaggtgt atcgtgagat caccaacaaa
     1501 ctgggcgaag agatcgcccg gttgtgtcag caagaaaagg agggatgaaa cattagtctg
     1561 atggagcatt cgggctgtat ttctctggag tagcgccgga aagggggtac tacccaacaa
     1621 aaagcatctt tcattttata gggagaggat agaaaatggc atcagtggac aatatgggag
     1681 aagaacggga cgacatcaat ggcttggaaa atgaagccga taattcaggt ctcgaagact
     1741 tcgacaagct gcaggagttt tatgacggct ccttcaagga gattcggagc ggacaggtcg
     1801 tcaagggcag agtcgtctct gtcaaacagg atgaggtcat cgttgacatc ggagccaagt
     1861 gtgagggcgc tatcgcgctc gaggagttca tgagcgacga caaagaccga aatgtctacg
     1921 tcggtcgtga gtttgaagtg ttcatcgtca agcgggaaga tcaggaagga gctcctgtgc
     1981 tttcccgtcg tagagcgaga gagatcgtca cacgagagaa catcaagcgc gcctatgcca
     2041 gtggcgagcc ggttgagtgt cgcgccatca aggtggtcaa gggaggactc caggtggact
     2101 gcgggatcaa cggtttcatg ccgttttccc atacgggg
//
LOCUS       DAOQUC010000713         2138 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_65124_length_2138_cov_2.683149, whole genome shotgun sequence.
ACCESSION   DAOQUC010000713 DAOQUC010000000
VERSION     DAOQUC010000713.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2138
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65124_length_2138_cov_2.683149"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            292..1752
                     /locus_tag="PKH07_11775"
     CDS             292..1752
                     /locus_tag="PKH07_11775"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNT35665.1"
                     /translation="MLTIAVLADTHRSTAPLANRRRGEIAHVLLLRAVHRLNRSIRPD
                     VTIIAGDILDAGQSSDADNLLSEMASILGLIESPKIVLPGNHDCSVEKFEQFLGLCPQ
                     WLDVANVRFVPFLDQQMSGYNACRSEADLERMAAARNGWPGELVTVQHVPLFPPGTHP
                     CPYNYTNADAILQVMDKQGYSLSVSGHYHHGMELMCQEGVHFMAAPALCDSPFGFSVL
                     RIENDGLSEQRHALRMPDNLRLFDCHVHTPFAYCNENMQFSTALELGEAFGLGGLAFC
                     EHSGQLYFGADDFWSGRFAQKGILGADEADNRMSTYLQTALSRATPSEVGLEVDCDFR
                     GNPVLKEQDRSSVSLLIGSIHKLSEFGKPNPDRAVACQEFLFMLERFLKSGIEVLAHP
                     FRVFVRSKQEKPECLFEPTASLLRDNGVAAEINFHKNQPPHAFVEMCLNMRVKLALGS
                     DSHNLYEVGEFAPHLEFLEQLGCLSQLESVLFRKQE"
     gene            1870..>2138
                     /locus_tag="PKH07_11780"
     CDS             1870..>2138
                     /locus_tag="PKH07_11780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016482573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNT35666.1"
                     /translation="MNQQTSRPNILFFFSDQQRWDTMGCYGQPLPVTPHMDKMASEGV
                     QFEYAFTCQPVCGPARACLQTGKYATEVGCFRNGIALPLDEPTLAR"
BASE COUNT          467 a          546 c          583 g          542 t
ORIGIN      
        1 tcacgaccac tgctgtgtgc tccaaggata tttcgatgtc ctcggttttc catccaccgt
       61 aaccttcggc cggaacgcac agcgagaagt ccgcgtcgaa ttgttgatag taactggccg
      121 gaactgagag cgttttcatg ttgaccaaga tccttgaccg ccttttctct gccagataga
      181 aaaagccatt tcgggatgat aaccagacaa agaccttttg gcaacgttcc gcagtacctt
      241 gagtttgtgt tagagttccc caaaagtgtt ttaggatgag gtaaggaatt catgctgacc
      301 attgccgttc tggctgatac gcaccgaagc actgcgcccc ttgcaaacag acgccgaggt
      361 gagatagccc atgtgctttt gcttcgtgca gttcatcggc tgaatcgttc cattagaccg
      421 gacgtgacca tcattgcggg ggacatcctt gatgccggcc agtcgtcaga tgccgacaac
      481 ttgttgtctg agatggcttc gattctcggc ctgattgagt ctccgaagat cgtgcttccc
      541 ggaaatcatg actgttcggt ggagaagttt gagcagtttt tggggttgtg tccgcagtgg
      601 ctggacgttg cgaatgtccg cttcgtccct ttccttgacc agcagatgtc cggctacaat
      661 gcctgtcgtt cggaagctga tctggagcgc atggcagccg cccgaaatgg ttggcctgga
      721 gaactcgtca cggttcagca cgtaccgttg tttccaccgg gcactcatcc gtgcccttac
      781 aactacacca atgccgacgc gatactccaa gtcatggaca agcagggcta ttcactttcc
      841 gtcagtggtc attatcacca cgggatggag ttgatgtgtc aagaaggggt tcatttcatg
      901 gctgcccctg cgctctgtga cagcccgttc ggattctccg tgttgaggat agaaaacgac
      961 ggattgtctg aacaacgtca cgcgcttcga atgcctgaca atctgcgact ttttgattgc
     1021 cacgttcaca ctccctttgc ttactgcaac gagaatatgc agttctccac ggcgttggaa
     1081 cttggagagg cctttggtct cgggggcttg gcgttctgtg agcattcggg acagctctat
     1141 ttcggggcgg atgatttctg gagtgggaga tttgcccaga aaggcatcct gggagcggat
     1201 gaagcggaca ataggatgag cacctatctg caaactgcac tctcccgggc aacgccgagc
     1261 gaagttggct tggaagtgga ctgtgatttt cggggcaatc ccgtgctcaa ggaacaagac
     1321 cggagttccg tgtcactcct gatcggctcg attcacaagt tgtctgaatt cggcaagccg
     1381 aatcccgatc gtgccgttgc ctgccaggag tttcttttca tgctcgaacg cttcttgaag
     1441 agtggcatcg aggtattagc ccatccattt cgagtgttcg tccgatccaa acaggaaaag
     1501 cccgaatgtc tgtttgagcc aacggcaagt ctgctgcgag acaatggcgt ggcagcggag
     1561 atcaacttcc acaagaacca gccgcctcat gccttcgtcg agatgtgctt gaatatgcgt
     1621 gtcaaactcg ctttgggaag tgattcacat aacctctacg aggtcggaga attcgcccca
     1681 catctggagt tcttggaaca gctcggttgt ctgtctcagt tggaaagcgt cttgtttcgc
     1741 aaacaggaat aggtgatgcg ggcacacgac agcgcacccg gacggatggt tcagccgaat
     1801 gcacggtttg catgttgcgt atgactgcac tattctctta gggagaataa ctgccttgga
     1861 gggcaataga tgaaccagca gacttcacga ccgaatattc tttttttctt ctccgatcag
     1921 cagcgatggg acacgatggg ctgctatgga caacctttgc ctgtgacacc ccacatggac
     1981 aagatggctt cagaaggggt gcagttcgaa tatgccttca cctgccaacc cgtttgcggt
     2041 ccggctcggg catgtttgca gactgggaaa tatgcaacgg aagttgggtg ttttcgaaat
     2101 ggcatcgcct tgcctctcga tgaaccaact ctggcacg
//
LOCUS       DAOQUC010000714         2135 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_65256_length_2135_cov_3.816346, whole genome shotgun sequence.
ACCESSION   DAOQUC010000714 DAOQUC010000000
VERSION     DAOQUC010000714.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65256_length_2135_cov_3.816346"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..179)
                     /locus_tag="PKH07_11785"
     CDS             complement(<1..179)
                     /locus_tag="PKH07_11785"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNT35667.1"
                     /translation="MAKSKSGKFRVGIIGSGGIARMSHVPGFQACPEAEIVAMCDINI
                     ERAKSAAEAAGIPRW"
     gene            443..538
                     /locus_tag="PKH07_11790"
                     /pseudo
     CDS             443..538
                     /locus_tag="PKH07_11790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002614994.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
     gene            1070..>2135
                     /locus_tag="PKH07_11795"
     CDS             1070..>2135
                     /locus_tag="PKH07_11795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35668.1"
                     /translation="MIKQQHLLKYLVIAAVCFIHFPTNACEIPVYEYALRFWSQDDYL
                     FLIYHKGVLSESQQSLADSLTQSASDTANFLLRTVDLETNEDPQVLSLLKEMPESADL
                     PRMSVHYPISARISKELFSMKLSRESVEILLNSPLRSEIARRLMAGDAAVWVLLESGE
                     GKADDSAAQLIQSVFEESDFSPETTDSFSDADSDVVEGVASDAEPAFSLLRLPRNEPE
                     ERTLIELLLNTESDLKDASAPIAFPIYGRGRVLYALVGKGITRRNVEEACRFITGPCS
                     CLAREDNPGIELLMNFNWESAVEKQIGQAEIPMSLSSVSSLIEAVSPEPIVVTQTLSV
                     QRETQRAGRPLKRNMTVALVG"
BASE COUNT          486 a          567 c          566 g          516 t
ORIGIN      
        1 taccatctcg ggattcctgc tgcttcagca gcactctttg cccgttcgat gttgatgtcg
       61 cacatcgcga caatctccgc ctcgggacat gcctgaaaac caggcacatg cgacatacga
      121 gcgatgccac ccgaaccgat gattcccact ctgaacttgc cggacttcga cttggccatg
      181 acacaattct ccttcgatat agaagatgat gacactatgg aatttcgaac acggctgaat
      241 atcgccaagt taagagaggg atgtcaagct gaaacaacag ggagtgccac gatgcctccg
      301 cccatgttgc aacgcgccca tgctcaaccg gaagagtcac ccgttgcgac tttgtgctat
      361 tctcttgttg ccggcctgtg ccagccggca ggtggattat gaccaccgct cacacgcatg
      421 ttgacgagag gagtacgacg tcttgtctgg aaaacgatct gccggtttca ctcttattga
      481 actcttgatt gttgtggcga tcattgggat tctcgctgca atagcgattc caaatttcct
      541 gttggctcag gttcgggcaa aggtatctcg tgtgaaggcg gacatgaacg gcctggccgt
      601 cgctctggag tcctaccgag tggatcaaaa cgcgtatccc tacttcgaca acccgaccta
      661 tggctttccc tctcggtaca acgagatcag ctaccgactg attcatctga caactcccat
      721 ggcctatttg aagacaattc atctgagcga tccctttcta gagcaaggtg cggacagtta
      781 cagcgatggg tttatgagat atgagtacaa ctatcgcaat catgaagtct ggcgttccgg
      841 aagctatccc tgggtgaagt gttccgcctg ggttctcaac agtatcggcc ccgatcagtc
      901 tcccaaccaa gggcttctga cagagatgtg ggcgcgggga ctcatggacg gtgttgcggg
      961 ggttgaagtg atcatctacg acccaactaa tggaaccgtg agtgtcggtg acattccccg
     1021 aaccggcggt gagacccgtt ttcgttcgtt cgctggcagg tgaaggcgta tgattaagca
     1081 acagcatctt ttgaagtatc tcgtcatcgc cgctgtgtgc tttattcatt tcccaacaaa
     1141 tgcctgcgaa atccccgtat acgagtatgc cctgcgcttt tggagccagg atgattatct
     1201 gtttctaatc taccacaagg gcgtcttgtc agagtcccag caatcactgg ccgacagcct
     1261 gacccagtct gccagtgaca ccgccaattt ccttttgcgt acggttgatc tggaaactaa
     1321 tgaggatccg caggttctct ctcttctgaa agagatgccg gaatcggccg acttaccacg
     1381 gatgtccgtc cactatccca tcagcgccag aatctccaag gaactcttct caatgaaact
     1441 ctccagggag tctgttgaga ttcttctgaa ctcgcctctg cgcagtgaga ttgcacgccg
     1501 tttgatggcg ggtgacgctg cggtctgggt gcttctggag agtggcgagg ggaaggccga
     1561 tgatagtgca gcgcagttga ttcagagtgt ctttgaggaa tcagatttca gccctgagac
     1621 aactgactcc ttctccgacg cggattctga tgtagtggaa ggtgtcgcct cagatgcaga
     1681 gcctgcattc tcactgcttc gtctccctcg aaacgagccc gaggagagga cactcattga
     1741 actgcttctg aacacagagt cggatttgaa ggatgcctcc gctccgattg ccttcccgat
     1801 atacggtcgc ggaagagttc tctatgcgct tgtgggaaag ggcatcacac gcaggaatgt
     1861 cgaagaggcc tgtcggttta tcaccggtcc gtgctcctgt ttggcacggg aagacaatcc
     1921 aggcatagaa ctcctgatga atttcaactg ggaaagcgct gtggaaaaac aaatcgggca
     1981 ggcagagatt cctatgtcct tgagcagcgt atcatccctg atcgaagcag tgtcgccgga
     2041 accgattgtc gtgacgcaga ctctgtccgt tcaacgggag acacaaaggg caggcaggcc
     2101 gctcaagaga aacatgaccg tcgcacttgt cgggt
//
LOCUS       DAOQUC010000715         2131 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_65443_length_2131_cov_3.994701, whole genome shotgun sequence.
ACCESSION   DAOQUC010000715 DAOQUC010000000
VERSION     DAOQUC010000715.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2131
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65443_length_2131_cov_3.994701"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..821)
                     /locus_tag="PKH07_11800"
     CDS             complement(<1..821)
                     /locus_tag="PKH07_11800"
                     /inference="COORDINATES: protein motif:HMM:NF014072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LarC family nickel insertion protein"
                     /protein_id="HNT35669.1"
                     /translation="MNLYLDCFSGIAGDMYLAALLDLGLDISELRKFLEPFEIPQLEL
                     KSSRVLKKGIEATYLHVHVGGADSEHGHHHHHDHHGHTFNDIVAFLRRQSIAPKLIQR
                     TERVFRFIADAESKVHGKPLDELHFHEVGQWDAIVDVVGVLWGLERLGVKEVYASAVN
                     VGGGFVQFSHGCYPVPAPATANILQSAAIPFFTTPDVGEMLTPTGAALLAETVTAFTS
                     PGTMKVQKVAYGAGSRDIGNIPNVVRAMALESTDTQADRVVVIETNLDDMNPEFC"
     gene            complement(854..>2131)
                     /locus_tag="PKH07_11805"
     CDS             complement(854..>2131)
                     /locus_tag="PKH07_11805"
                     /inference="COORDINATES: protein motif:HMM:NF019934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspG"
                     /protein_id="HNT35670.1"
                     /translation="IYFSDGSFLRCKVHDEPIVFPRKKHPRFVEIEIPFGVIGFYMGE
                     HIVKVEKDDLYSPDERSLSLVRIMDLLGTSPADMMSLRTNDEAQGVRNLRLEVERVAG
                     WAFQRQGDRRLALTNGQEVPSDSLISVTRNGRLKVRFRDTGTVDAIQVVAGLLSETTA
                     EIQQTSYDEQSKTNILGVNLTAGKLWIDARDGLSEGRRTELFVSPIRVRCETSTYYVQ
                     LLPDQTVKVALLKGADLSVRWKDVPTEHAIEQGRYAIFGEDSRMRLEEGKVAADEFRE
                     WTNWDSWEALKVEVPLSVIIPPMVSFPSEGPEIVSVRAEETWRALRASQPLKTENFAR
                     KLQRTREAIAAYKNDLGEIPTMELGLQALLTRPDDPRWKGPYLPEDEAFEDFWGRPLI
                     YQIWTDLDGTPIADVRSMGMNGKDELGLGDDLR"
BASE COUNT          495 a          618 c          536 g          482 t
ORIGIN      
        1 tcgcagaact caggattcat gtcgtcaaga ttggtttcga ttacaacaac gcgatccgct
       61 tgagtgtcgg tggactcaag agccatcgca cgaacaacgt tggggatgtt tccaatgtca
      121 cgactacctg ctccgtaggc aaccttctgc actttcattg ttccaggtga cgtgaacgct
      181 gtgaccgtct cggccagcag cgcagcacct gtcggtgtga gcatctcccc gacatcgggg
      241 gtggtgaaaa atgggattgc ggcgctttgg agtatgtttg cggtggcagg cgcaggaacc
      301 ggatagcatc catggctaaa ctgaacgaac cctccaccca cattgactgc ggaggcgtag
      361 acctctttta ctccaagccg ctccaaaccc cacagcacgc caacgacatc cacgatggca
      421 tcccattgac caacttcatg gaagtgaagt tcatccagcg gtttgccatg aactttggac
      481 tccgcatcag caatgaaccg aaagactctc tcagtacgtt ggatcaactt cggagcgata
      541 ctctgtctgc ggagaaatgc gacaatgtca ttgaaggtat gaccatgatg atcatggtgg
      601 tgatgatggc catgctctga gtcagctcca ccaacgtgga catgaagata ggttgcctct
      661 atacccttct tgagaacacg actgctcttc agctccaact ggggtatctc aaacggttca
      721 aggaattttc tcagttcgga aatgtccaga cccaaatcca ggagcgcggc aaggtacata
      781 tcccccgcga tcccagaaaa gcaatcgaga tatagattca tgagaaacct caagtcgaat
      841 caggatggtg gtcttagcgc aggtcgtcgc ccagcccgag ttcgtccttt ccattcattc
      901 ccatcgaacg aacatccgcg attggggtgc cgtccaaatc cgtccagatc tggtagatca
      961 gcggtctacc ccagaagtct tcaaaggctt catcttcggg aaggtaggga cctttccatc
     1021 tgggatcatc cggtcgtgtc agcagagcct gaagccccag ctccatggtt ggaatctcac
     1081 ccagatcgtt cttgtaggca gcgatggctt cgcgagtgcg ctgaagtttt cgcgcgaaat
     1141 tctccgtctt caatggctgg cttgcgcgaa gggcgcgcca ggtttcttcc gcacgcaccg
     1201 agacaatctc aggcccctct gacggaaatg aaaccatggg cgggatgatg accgagaggg
     1261 gaacctcgac tttcagcgct tcccaggaat cccagtttgt ccattctcta aactcgtccg
     1321 cggccacttt gccttcttcg agacgcatgc gactgtcctc tccaaagatg gcgtagcgcc
     1381 cttgctcgat agcgtgctcg gtcgggacgt ctttccatcg aacgctcaag tccgctccct
     1441 tcagcagcgc gaccttgacc gtttgatctg gcagcaactg aacgtagtag gttgacgtct
     1501 cacaccgcac tcgaattggc gatacaaaga gctctgttcg gcgtccttcg gacaggccgt
     1561 ctctggcgtc tatccagagc ttgcccgccg tgagattgac gccgagaatg ttggtcttgg
     1621 actgttcgtc atagctggtc tgctggatct ctgccgtggt ctcggacaga agtccggcaa
     1681 cgacctgaat cgcatcaacg gttccagtgt cccggaaccg gactttcaga cgcccgtttc
     1741 gcgtgacaga aatcagggaa tccgacggca cctcctgacc attcgtcaga gcaagacgcc
     1801 tatccccctg tcgctgaaac gcccatccgg caacacgttc gacctcaagc ctcagatttc
     1861 gaaccccctg cgcttcgtca ttggttcgaa gcgacatcat gtccgccggc gatgttccaa
     1921 gcagatccat aatccgaacc agcgaaagag agcgttcatc agggctgtaa agatcgtcct
     1981 tctccacctt gacaatgtgt tcgcccatat agaacccaat gaccccaaaa ggaatctcta
     2041 tctccacaaa ccgcggatgc ttctttcggg gaaagacaat gggctcatca tgcaccttac
     2101 agcgcaagaa cgaaccatcg ctgaaataaa t
//
LOCUS       DAOQUC010000716         2130 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_65509_length_2130_cov_2.734940, whole genome shotgun sequence.
ACCESSION   DAOQUC010000716 DAOQUC010000000
VERSION     DAOQUC010000716.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2130
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65509_length_2130_cov_2.734940"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1412)
                     /locus_tag="PKH07_11810"
     CDS             complement(<1..1412)
                     /locus_tag="PKH07_11810"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNT35671.1"
                     /translation="MSLGTRIGIGFACLILIAAALGTIAVWNMTNVEEHARELSTEDI
                     PMVALGNNVERHSLKTMFAMRGYMYTGNEVFWTEGQKELQDVRKYLEDIRKLAEKSTD
                     QQSALAAAKQMKDDAEHYSRLATDTQGTIVRLSELHTLMDEQSALYQQEAAAYLKTMG
                     DTLISEIVEGKEMSTLIERFSKVIWGNEIIALGNEVHIASLQAEARRDPKTIQEALPV
                     FDKMEKLLDEVEALTRQEANRRQLEKIRAAARQYKQAMLDTLQAWDEQTKLNEDRGKV
                     VNNVLAGAEKIAQDGMKACTLVADEAVASLHRSSTIMISGLVVALIVGVLMAVFITRS
                     ITKPINAVIESLRAGANQVSAASGQVSESSQQMAEGASEQASSLEETSSSLEEMASMT
                     RQNADSANQCNSLMGEAKQVVGEMAHAMEDMSSAIQKIKTSSNETAKIIKTIDEIAFQ
                     TNLLALNAAVEAARAGEAGKG"
     gene            1702..>2130
                     /locus_tag="PKH07_11815"
     CDS             1702..>2130
                     /locus_tag="PKH07_11815"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HNT35672.1"
                     /translation="MRRDQAPSDALEGKSIEVDYLCIYQPPRRLKYWVVLMKAPISFL
                     TIGVLSALFMIGCDMDGKRPVMRDALLLGTWVRIAVVSDDTQAAEKAIDRAIEKMRQV
                     EDTCSFHDPESELSHVNIRAATEPVAVSDCFWSVLSHAVQA"
BASE COUNT          445 a          593 c          548 g          544 t
ORIGIN      
        1 cctttgcccg cctcaccggc acgcgctgcc tcaactgctg cgttgagagc gagcaggttt
       61 gtctgaaatg cgatctcatc aatagtcttg atgatcttgg cagtttcgtt tgacgacgtc
      121 ttaatcttct ggattgccga tgacatatct tccattgcat gcgccatctc accaacgacc
      181 tgcttggctt cacccatcag ggagttacac tggttggcac tgtccgcgtt ctgtcgcgtc
      241 atggaagcca tttcctccag agatgaggaa gtctcttcga ggctagatgc ctgttcactc
      301 gccccttctg ccatctgttg gctggattca gaaacctggc cagaggcagc gctgacttgg
      361 tttgctccag cccgcaatga ctcgatgacg gcattgatgg gcttggtgat ggagcgagtg
      421 atgaacacag ccataaggac accgacaatc aaggccacaa ccaatccgga gatcatgatg
      481 gttgaggaac gatgcagtga agctaccgcc tcgtcagcga cgagcgtaca ggccttcatt
      541 ccatcctggg cgatcttctc agcgcccgca agcacattgt tgaccacctt gccacggtcc
      601 tcattgagct tggtctgctc atcccaagcc tgtagcgtgt cgagcatcgc ctgtttatac
      661 tgtctggcgg ccgctcggat cttctccaac tgtctccggt ttgcctcttg gcgcgttaag
      721 gcttcgactt cgtccaagag tttctccatc ttgtcgaaaa caggcagtgc ctcctggatt
      781 gtctttggat ctcttcgcgc ttccgcctgg agagaggcga tgtgcacctc gttgccaaga
      841 gcaatgatct cgttgcccca tatcaccttg ctgaatcgct cgatcaaggt ggacatctct
      901 ttgccttcca caatttcgga gatgagtgta tcacccatag ttttcaggta ggctgccgcc
      961 tcttgctgat agagcgcaga ctgctcgtcc atgagcgtgt gcagttcgct gagcctgacg
     1021 atagtgcctt gggtatcggt cgcaagccgg ctgtagtgct ccgcatcgtc tttcatctgc
     1081 ttggctgccg caagcgcaga ctgctgatcg gtggacttct ccgccaactt ccgaatatct
     1141 tccaggtact tacgcacgtc ctgaagctcc ttctgtcctt cagtccagaa gacctcgttt
     1201 cccgtataca tatagccccg catggcgaac atggtcttca gggagtgtct ctcaacgtta
     1261 ttccccagcg ccaccattgg aatgtcctct gttgagagct ctctcgcatg ctcctccacg
     1321 ttggtcatgt tccagacggc aatggttccc agagcggctg cgatgagaat caaacaggcg
     1381 aagccgattc cgattcttgt ccccaaactc atgactagat cctccttaat gccttgtagt
     1441 ggcaatgaaa ccaagctgcc agagcgctcg agcagcgacg agacaactcc aatagcgata
     1501 aacgagttgt tcaggagacg cagtctctct tcgatgaacc cgctcactct cgattcagaa
     1561 ctaccgaaag gcaaatcccc aaaggacgat agcgccctga ggacaaagcc ttggttcata
     1621 tccaaacagt accgcgaatg ctgtcgaggt gcaaatgtat ttgtcttgac tgatcccttc
     1681 tgttgacaca gccggttttg catgagaaga gatcaagcac cctcggatgc tcttgaaggg
     1741 aagagcatag aggtggacta tctctgcatt taccagccgc cgaggcggct gaagtactgg
     1801 gtggttttga tgaaggctcc gatttcgttc ctgaccatcg gtgtcctctc tgcactgttc
     1861 atgatcggct gcgacatgga cggcaagaga cccgtgatgc gcgatgcgct tctgcttgga
     1921 acctgggtac ggattgcggt tgtcagtgac gacacgcagg ctgccgagaa ggccattgat
     1981 cgagcaattg agaagatgag acaggtcgaa gacacatgca gttttcacga tcccgaaagt
     2041 gaattgagtc atgtgaacat tcgtgcagcc acagagcctg ttgccgtttc tgattgtttt
     2101 tggtctgtcc tctctcatgc ggttcaggcg
//
LOCUS       DAOQUC010000717         2129 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_65537_length_2129_cov_3.610897, whole genome shotgun sequence.
ACCESSION   DAOQUC010000717 DAOQUC010000000
VERSION     DAOQUC010000717.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2129
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65537_length_2129_cov_3.610897"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1456
                     /locus_tag="PKH07_11820"
     CDS             <1..1456
                     /locus_tag="PKH07_11820"
                     /inference="COORDINATES: protein motif:HMM:NF036810.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HNT35673.1"
                     /translation="EVAPGLQQWGVMLPYTPLHEMLLQEIRRPIVLTSGNLAEEPIVH
                     RDDEALERLNSIVRLYLVHDRDIATRCDDSIVRVWSGGSIVLRRSRGFVPAAIDVPVS
                     ASKRIFACGAQLKNTFCLLGTTPDASAGDNTDSIHPFPRGAQAVLSQHIGDLESLEAQ
                     SGYEEAVRHFFGLFDFKPEVIAHDLHPDFFSTRFLGSFRDVPRIAVQHHHAHLASCLA
                     ENCRQQPAIGVCWDGIGLGLDGSAWGAEFLVGDLKDFRRAGTFRPIPIPGGDVAAREP
                     WRMGLAYLRDAFGNRWRNESKGFFENFHSGEMNIVQQMLESDVNCPCGSSMGRLFDAM
                     SFLCLGIHRTTYEGQAALWLEQAAAQAAPTQDALPFEVDVHDDLLIVEPSKAILDAVA
                     RIRARTSASDVARIFHNGLCQVVVDVCLRLKSNGAPNCVALSGGCFQNRLLLDLCSAR
                     LVQEGFEVLTHHSVPPNDGGIALGQAVIAAARIG"
     gene            1546..1785
                     /gene="hypC"
                     /locus_tag="PKH07_11825"
     CDS             1546..1785
                     /gene="hypC"
                     /locus_tag="PKH07_11825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004556659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HNT35674.1"
                     /translation="MCRAIPHRVIQVAGEKGLVEVGGVRHWVDLSLVAVDDDSVRVGD
                     FVLVHSGFAISVLDETAAEQTLDLIRELAAAGEHV"
     gene            1782..>2129
                     /locus_tag="PKH07_11830"
     CDS             1782..>2129
                     /locus_tag="PKH07_11830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35675.1"
                     /translation="MIRTFRYILLIGLLFGLQAGTRFAADFWGPMPQSFEPCEINIPP
                     RVSARRIGRILQNHQVVPSALHFLVSAQYLGLARKLNPGTFRFDRPMPVWDVVRQIAT
                     YVPNTYWVTIPEGF"
BASE COUNT          383 a          557 c          639 g          550 t
ORIGIN      
        1 ggaggtcgct cccgggttgc agcagtgggg agtgatgctt ccttacacgc ctctgcacga
       61 gatgcttctt caggagatcc gtcggcctat cgttttgacg agcgggaatc ttgctgaaga
      121 acccatcgtt catcgagatg acgaggccct tgagaggctc aactcgattg tgcggttata
      181 tctcgttcat gacagggaca tcgcgactcg ctgtgacgat tccattgttc gtgtttggtc
      241 agggggctcg attgtgttgc ggcgatcgag agggtttgtg cctgctgcga tcgatgtacc
      301 agtctcggcc tctaagcgga ttttcgcctg tggagcgcag ttgaagaaca cgttttgtct
      361 cttgggcaca acaccggacg cttctgcggg tgacaatacg gattcgattc atccttttcc
      421 gcggggagca caggcagtgc tctcgcaaca cattggagat cttgagagtc tggaagctca
      481 gtccggctat gaagaggctg tcaggcactt tttcggcctt tttgatttca aacctgaagt
      541 gattgcccat gatctgcatc cggacttctt ctcgacacga ttcttgggca gttttcgtga
      601 tgttccgcga atcgcggttc agcatcacca cgcacattta gcatcctgtc tggctgagaa
      661 ttgtcgtcag cagcctgcca tcggcgtttg ctgggacggg atcgggctgg gtctcgatgg
      721 ctccgcctgg ggagccgagt ttcttgtcgg cgatctgaag gatttccgtc gggcgggcac
      781 cttccgaccg attccgatac ccgggggaga tgttgcagca cgtgagccgt ggcgcatggg
      841 gctcgcctac cttcgagacg cgtttggaaa ccgttggcga aatgagtcaa aagggttttt
      901 tgagaatttt cattcgggtg agatgaacat tgtccagcag atgcttgaat cggacgtcaa
      961 ctgcccttgc ggttcgagca tgggacgact ttttgatgcc atgagtttcc tgtgccttgg
     1021 aatccatcga accacctatg aaggccaagc tgctttatgg ctcgaacagg ccgcggcgca
     1081 ggcagcacca actcaagacg cactgccgtt cgaagtagat gtccacgatg atctcctcat
     1141 cgttgagcca tccaaggcga ttcttgatgc cgttgccagg attcgcgccc gtaccagcgc
     1201 ttcagatgtg gctcggatct ttcacaacgg tctctgccag gtggttgtag acgtttgtct
     1261 ccgtctgaag tcaaacggag cacccaactg cgtcgctctc agtgggggtt gtttccagaa
     1321 ccgattgttg ctagatttgt gctcggcgcg cctcgttcag gaaggctttg aagtgctgac
     1381 ccatcacagc gttcctccaa acgatggggg gatcgccctg ggtcaggccg tcattgccgc
     1441 agcgcgtatc ggttagttgg cggtgggctg cgatttgtcg gctgttgtgc ccaatcacag
     1501 acaacagatg ggtcatgacc gttccgcaga gtttggaggc ttgtcatgtg ccgtgccatc
     1561 ccgcatcggg tcattcaggt tgccggcgaa aagggtctcg tcgaagtcgg cggtgttcgc
     1621 cattgggtgg acttgtctct tgtagcggtg gatgatgatt cggtgcgtgt gggtgatttc
     1681 gttctcgtgc attcgggttt cgccatctcg gttctcgacg aaaccgccgc ggaacaaact
     1741 ctggatctga ttcgggaact ggcggcggcg ggagagcacg tgtgatccga acgttccgat
     1801 atattctctt gatcggcctg ctgttcggac tgcaggcggg gactcgcttt gcggcggatt
     1861 tctggggacc gatgccgcag tcttttgagc cctgcgaaat caacattccc cctcgggtgt
     1921 cggcgcgacg aatcggaaga attctccaga atcatcaggt cgttcccagt gccctgcact
     1981 ttctcgtctc ggcgcagtac ctggggttgg cgcggaaact gaaccctggt actttccgtt
     2041 ttgaccgtcc catgcccgtt tgggatgtgg tgagacagat cgccacctat gttcccaaca
     2101 cctactgggt cacgattcca gagggcttc
//
LOCUS       DAOQUC010000718         2127 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_65625_length_2127_cov_3.698842, whole genome shotgun sequence.
ACCESSION   DAOQUC010000718 DAOQUC010000000
VERSION     DAOQUC010000718.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65625_length_2127_cov_3.698842"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..758)
                     /locus_tag="PKH07_11835"
     CDS             complement(<1..758)
                     /locus_tag="PKH07_11835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35676.1"
                     /translation="MAKEKADVVVSWYFENAETQALRDRFTRPTRFLQMAAGPGTKYD
                     TQLLDYGKTSIQNLLRAKLPNVEPQRVAVMGFSEGCRGVREALKCGDAGRLDAVFGID
                     GIHTQWVKPRETFETGLLLPWRAIAKKAVNGGPLVVITTSDVQPTYTDTTTTSNWIWE
                     QATGTNEIFFDLEIPSWLMGPVEPPYTSSPGSFGPGQQSWKETVYTQYPLRVFRKSGG
                     LWISNYYDLDPTGVGDHRFQAARVSPLHYLHVLI"
     gene            1230..>2127
                     /locus_tag="PKH07_11840"
     CDS             1230..>2127
                     /locus_tag="PKH07_11840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HNT35677.1"
                     /translation="MSKTASRDPLVRAWTVQKIHNARLAGLPTDTDEEKAGIILSAVQ
                     QEFTYVADPLFSEMMVAPKWMIQGIIPGGDCDDLVGLTVACFLAALSSAGVRCAVIGH
                     SYQKHMDIEHVLCAVFLNKRWLYADPCSKFPLGRTAAPHTWEIVFDPMFPEKPFCDAT
                     SCLVGSNAVPPPVFRESHGDFVGVSGLPTPPEVMVIDPLEEDPVATEMWLEEDTEMTH
                     LKSPQSLLREISESPDARGEIAETPVQLARPVLDEMTVTNGMSGRHMGGLFEALPFQV
                     PTLLDWPVDMPPVAPQPTMMDVP"
BASE COUNT          497 a          591 c          525 g          514 t
ORIGIN      
        1 tcaatgagca catggaggta atgaagcgga gacacgcggg ccgcttggaa tcgatgatca
       61 ccaacacccg tgggatcgag atcgtagtag ttgctgatcc acaatccgcc gctttttcga
      121 aacacgcgca gcggatattg cgtgtaaaca gtctccttcc aggactgctg tcctgggccg
      181 aacgatccag gtgagcttgt gtaaggtggt tcgacaggtc ccatcaacca agaaggtatt
      241 tccagatcaa aaaaaatctc gttggtccca gtcgcctgtt cccagatcca attggaagtc
      301 gtcgtcgtgt cggtgtacgt cggctggaca tcgctggtcg tgatgacaac cagaggtcct
      361 ccattgacgg ctttcttggc aatcgctcgc caaggaagca agagaccggt ctcaaaggtc
      421 tctcgcggct tgacccactg tgtgtggatc ccatcaatgc cgaaaactgc atccagcctc
      481 cctgcatctc cgcacttgag ggcctcacga acaccacgac atccttcact aaatcccatc
      541 accgcaactc gttgcggttc cacattgggc aacttggctc gcaacaaatt ctggatagat
      601 gtcttcccat aatccagaag ttgcgtgtca tacttggtgc ccggcccagc ggccatttgc
      661 agaaatcgag tgggtctggt gaatctatcg cggagagctt gtgtctcagc attctcaaaa
      721 taccacgaca ccacaacgtc tgctttctct ttggccatta gaatatcttg cctcaattca
      781 aggcagacga caaccaattg gttctgctac aatccatgtc atgtccctcg gagtcagcgc
      841 aaaacaaacc aagatagacc tgggtcactc cctgttctct tcactcacgt tggtcgcact
      901 tcttttttat ccattcttgg cgttcgctga cattcctgtt ccaacgacct cttcatcttc
      961 gttcaggcat cgagaagatg ctccagacaa acagggacaa gtcagagaag ttgagtatct
     1021 tccacgacca actttcccag acacacgacc acttttgttc gccagtctgt tgctccttgg
     1081 aatctcggca cttcggccca acccacaatc aaaaaggaac tcctgacatg gcgctagaac
     1141 tccaagaaag cccccgtttc gaggttcacc ggaccaagat cttgtctggg aatcgaggta
     1201 gtcggtggtc gatcaaaaag acagccgagt tgagcaaaac ggcctctcgc gatcctctgg
     1261 tcagagcctg gacggtccaa aaaattcaca acgcacgtct tgctggattg ccaacagata
     1321 cggatgaaga aaaagcgggg atcatcctct ctgcggtcca acaagagttt acttacgtcg
     1381 cggatccact tttttccgag atgatggtgg ctccgaagtg gatgatccag ggcatcattc
     1441 ctggcggaga ttgcgacgat cttgtcgggc tcacagtcgc ctgtttcttg gccgcgctca
     1501 gttccgccgg cgttcgatgc gctgtgattg ggcactctta ccagaaacac atggatattg
     1561 aacatgtgct ctgtgctgta ttcttgaata agaggtggct ttacgcagac ccgtgctcga
     1621 agtttccact gggccgaacc gcagcgcctc atacatggga aatcgtgttc gatccgatgt
     1681 tcccagagaa gccattctgc gatgctacat cttgcctggt tggatcgaac gccgttccac
     1741 cgcctgtctt cagggagtct catggagatt ttgttggagt atcaggcttg cctactccac
     1801 cagaagtcat ggtcattgat cctctggaag aagatcctgt agccacagaa atgtggttgg
     1861 aagaagatac cgagatgaca cacctaaagt ctccacaatc tctgttgcgc gagatctctg
     1921 aaagccctga cgcgagaggg gagatcgcag agactcctgt gcagttggct cggccagtcc
     1981 ttgatgagat gactgtgacc aacggcatga gtggacgcca tatgggcggc ctcttcgagg
     2041 cgcttccttt ccaagtcccg accttgcttg attggcctgt ggacatgccc cctgtcgctc
     2101 cacaaccaac catgatggat gtcccat
//
LOCUS       DAOQUC010000719         2126 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_65682_length_2126_cov_3.690488, whole genome shotgun sequence.
ACCESSION   DAOQUC010000719 DAOQUC010000000
VERSION     DAOQUC010000719.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2126)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2126
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65682_length_2126_cov_3.690488"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..540
                     /locus_tag="PKH07_11845"
     CDS             <1..540
                     /locus_tag="PKH07_11845"
                     /inference="COORDINATES: protein motif:HMM:NF018095.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNT35678.1"
                     /translation="ILNYERAQRLIPRYQDLQVNHKRVVELLEDEEFHKFQEGEAPLI
                     LRGLYRSMTLNELTLMIGVLLVAAAAVTILRWLWKRPSFQTLSNYLLSFLAIVVIILG
                     ATGSFRFRNEVLRSRAVVVSTEVDVRSGPGQDQGVVFTVHPGTLVQIHRRRENWVQVS
                     LPNGYSGWLPGTDVQEVSW"
     gene            696..>2126
                     /gene="groL"
                     /locus_tag="PKH07_11850"
     CDS             696..>2126
                     /gene="groL"
                     /locus_tag="PKH07_11850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012464512.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HNT35679.1"
                     /translation="MAAKMIKYDDAARARLLRGIETLTKAVRVTLGPKGRNVALEKKF
                     GSPTITKDGVTVSKEIQLENPYENMGARMVNEVASKTSTLAGDGTTTATVLACAIFKE
                     GIKNVTAGQNAMDIKRGIDQAVQVVVEHLSSIAKPVHDKQEITQVAAISANNDMFIGE
                     LISDAMEKVGKDGVITVEEAKSMDTTVEIVEGMQLDKGYISPYFATNPETLETELEDP
                     LILLYEKKISNMMEMVPLLEAVVQRGRSLLIVAEDVENEALATLVVNRLRGILKVAAI
                     KAPGFGDRRKAILQDMAVLIGGQVISEELGQKLENVQISDLGTAKRVSIDKETTTIVE
                     GGGSKKDIQARIAQIKMQIEETTSDYDREKLQERLAKLAGGVAIVRVGAATETEMKEK
                     KARIEDALHATRAAVEEGVVAGGGVALLRAIPKLEALKLEGDAKVGVEIVKRAIEQPM
                     RWIAENAGENGMMVVAQVKQKKGNIGF"
BASE COUNT          542 a          479 c          662 g          443 t
ORIGIN      
        1 atcctcaact atgaacgggc acagcgtctg attcccaggt atcaggactt acaggtcaac
       61 cacaagcggg tcgtcgagtt gctggaagac gaggagttcc acaagtttca agagggtgag
      121 gcaccgctga ttctgcgggg gctttaccgg agcatgacac tgaacgaatt gacgctgatg
      181 ataggtgttc tgctcgtcgc cgcagccgcg gtgacgatac tccgttggct gtggaaaaga
      241 ccttcttttc agacactttc aaactacctg ttgagttttc tggccattgt ggtaatcatt
      301 ctcggtgcga ctggctcttt ccggttccga aatgaggttc tcaggagtcg agcagtcgtg
      361 gtttcgacag aggtggatgt tcgcagtggg cctggacaag accagggcgt cgtgttcacg
      421 gttcatccag gaacgctggt tcagattcac cgtcggcggg agaattgggt tcaggtcagt
      481 cttccgaatg gctatagtgg ctggctgcct ggaacggatg tccaggaggt cagttggtga
      541 acgttgggga ttcgctgcgc agtgaatcgt ccttcggact tgtgcccaga gttgacagga
      601 agagagagaa gaaataaagg gtttggagtc tctctgttgt agttgatgaa gagaggtacc
      661 tcccaactca tttttgcagg aggagaagaa aacagatggc ggcaaagatg atcaagtatg
      721 atgacgcagc acgcgcaagg ctgctgcggg gaatcgagac actgaccaag gcagtccgtg
      781 tcacattggg acccaaggga cgcaacgtcg ctttggagaa gaaatttgga tcaccgacga
      841 tcaccaagga cggggtgact gtatcgaaag agatccagtt ggaaaacccc tacgagaaca
      901 tgggtgctcg aatggtcaac gaagtggcgt ccaagaccag cacgctcgcc ggagatggaa
      961 ccacaacggc aacggttctg gcctgtgcca ttttcaagga aggcatcaag aatgtcaccg
     1021 cgggacagaa tgccatggat atcaagcgtg gtattgacca ggcggttcag gtggtggtgg
     1081 agcatctcag ttctattgcc aagccggttc atgacaagca ggagatcacc caggtggctg
     1141 caatttccgc caacaacgac atgttcattg gcgaattgat ctctgatgcc atggagaaag
     1201 ttggcaaaga cggtgtcatc acggtcgaag aagccaagtc catggatacg acggttgaga
     1261 tcgtcgaagg aatgcagttg gacaagggat atatctcccc ttacttcgca accaacccag
     1321 aaacccttga gacagagttg gaagatcctc tgattctgct gtacgagaag aagatcagca
     1381 acatgatgga gatggttccc ctgttggaag cagtggttca gagaggccgc tccctactca
     1441 ttgtcgctga ggacgtcgag aatgaagcgt tggcgacact ggtggtaaac cgacttcgtg
     1501 ggattctgaa agttgcagct atcaaggctc caggatttgg cgaccgtcgc aaggcgattc
     1561 ttcaggatat ggccgtcctt atcggaggcc aggtcatcag cgaagagctg ggtcagaaac
     1621 tggaaaacgt ccagatatcc gatctgggta ctgccaagcg ggtctctatt gacaaagaga
     1681 cgaccacaat tgttgaaggt ggcggttcca agaaggacat tcaggccagg attgctcaga
     1741 tcaagatgca gatcgaagag accacatcag actacgaccg cgagaagctc caggaacgac
     1801 tggcaaagct ggcgggtggc gtggccatcg ttcgcgttgg agcagcgacc gagacggaga
     1861 tgaaagagaa gaaggctcgg atcgaggatg ctctgcacgc cacacgggcg gcagtcgagg
     1921 aaggtgttgt ggcaggcgga ggcgttgccc tgcttcgagc cattccgaaa ttggaagcgc
     1981 tgaaactcga aggggacgcc aaggttggtg ttgagatcgt caagagagcc atcgagcagc
     2041 caatgcgctg gatcgcggag aatgccggag agaacggcat gatggttgtt gctcaggtga
     2101 agcagaagaa gggcaatatc ggattc
//
LOCUS       DAOQUC010000720         2125 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_65741_length_2125_cov_3.168116, whole genome shotgun sequence.
ACCESSION   DAOQUC010000720 DAOQUC010000000
VERSION     DAOQUC010000720.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2125
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65741_length_2125_cov_3.168116"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1972
                     /gene="pilM"
                     /locus_tag="PKH07_11855"
     CDS             <1..1972
                     /gene="pilM"
                     /locus_tag="PKH07_11855"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HNT35680.1"
                     /translation="KKETLAVDLGQGCIKVLKLSPDRAGLRAETLVTADFGFDPALSP
                     EDSQKKRGQILASIWKEYKFRTKEVYLSVPGRGAIVRQFRIPRVTGDRLDRIVRFEAK
                     QQIPIPLDQVILDYHCFDSGEPELDVTLIAIRKTAIEEYVAVLDVAKLRPDVIDVAPL
                     CLFNALVDNEADPSEVVGLIDFGASETNIVIYQNKIVRFIRSAPVGGNVITESIQSQM
                     GFASFAEAEEMKLSLSSQRSSSKGPESLTADQQRATSVVESSLDRILGEVRRSIDFFI
                     SKPEGEALTRVLITGGTSRIPGIEERIEERLGVSVTKADTFELAKVETAPIEAQGLAE
                     VGPVVLGLGARAAGKSIVAMNFIPPSVKESKVLKQRTAYLVIQAALVLLTAFAGVLHI
                     NSQLKKYDEANQYLENIIHFEGVGDKAKDLLTELGKMNERFEKLKGVLNQRGRLSSML
                     FEIAKIAPPEIWLYQLRGSQRNFVLKGKCETRDAIKINEFIEVLNLNPFFSGIEVKTQ
                     VPKGDYVEFEIAVNELVTPDPNRMKLIDALRQKRMDFVFADLLLDQDKTAAYLVVYGS
                     DDQQANDQQWPEIVDALVSSGVHFDRVQVMWMNRLRKKLYEQRCRREFCEQLQKDELS
                     LEEFLKNVRFFEVKETKPEPQPGGEGVPPGGQ"
BASE COUNT          509 a          528 c          594 g          494 t
ORIGIN      
        1 gaagaaggaa acgcttgccg ttgatctggg tcaaggctgt attaaggtct tgaaactcag
       61 ccctgatcgt gcaggtttgc gggcggaaac tctggtgacc gccgattttg gttttgatcc
      121 tgcactctct cccgaagact cccagaaaaa gcgcggtcag attcttgcct cgatctggaa
      181 ggaatacaag ttccgaacca aagaggtcta tctgtccgtt ccgggtcggg gtgccatcgt
      241 ccgccaattc agaattccgc gcgtgaccgg tgaccgcctg gatcgcattg ttcggttcga
      301 ggccaagcag cagattccca tccccttgga tcaagtcatt ctggactatc actgttttga
      361 cagcggggaa ccggaactgg acgtcacgct gatcgccatt cgaaagaccg ccattgaaga
      421 gtatgtcgcg gttctcgacg tcgccaaact gcgaccggac gtcattgatg tggcaccgtt
      481 gtgtctcttc aacgcccttg tagacaatga ggcagatccc tccgaagtcg tgggtttgat
      541 tgacttcgga gcctctgaga caaacattgt catctaccag aacaagatcg ttcggttcat
      601 tcgaagcgct cctgttggtg gtaacgtcat taccgagtcc atccagagcc agatgggctt
      661 tgcctctttt gccgaagccg aggagatgaa actctcgctc agttcccaac gcagttcatc
      721 caagggacca gagagcctga ctgcggatca acagcgagca acctcggtgg tcgagagctc
      781 cttggacaga atcctgggcg aagttcgacg ctccatagac ttcttcatct ccaagccgga
      841 aggagaggct ctgacacggg ttctgattac tggtggtacc tcacgcattc caggcatcga
      901 ggagcgtatt gaggagcgtc tgggtgtttc tgtcaccaaa gccgatactt ttgaacttgc
      961 caaagtagag acagctccca tcgaagcaca ggggcttgcc gaagttggtc ccgttgttct
     1021 agggttgggc gcaagggcgg cgggcaaatc catcgttgcg atgaatttca tcccgccatc
     1081 tgtcaaggaa tccaaggtac tcaaacagcg taccgcgtat cttgtcatcc aagcggctct
     1141 tgttctgctg acggcgtttg ccggagtgct tcacattaat tctcagctga agaaatacga
     1201 cgaagccaac cagtaccttg agaacatcat ccatttcgag ggagttggag acaaagccaa
     1261 ggatctgctc accgaactgg gcaagatgaa cgaacgtttc gagaagttga agggtgttct
     1321 caatcagcga ggacgcttgt ccagcatgct gtttgagatt gccaagatag cgccacctga
     1381 gatctggctc tatcagctgc gaggaagcca gcgcaacttt gttctgaagg gcaagtgtga
     1441 gacccgcgat gccatcaaaa tcaatgagtt cattgaagtg ctgaacctga atcctttctt
     1501 ctcgggcatc gaagtcaaga cgcaggttcc caaaggggac tatgttgagt tcgagatcgc
     1561 cgtcaatgag ctggtgacac ccgatcccaa caggatgaaa ctcattgatg cgttgcgtca
     1621 gaagagaatg gatttcgtgt ttgcggatct gcttctggat caggacaaga ctgcggcata
     1681 cctggtagtc tatggatctg acgatcaaca ggccaacgat cagcaatggc ccgagatagt
     1741 ggatgctctt gtctcgtcag gcgtacactt cgaccgtgtt caggttatgt ggatgaaccg
     1801 gttgagaaag aaactctatg agcagcgctg ccggcgtgag ttttgtgagc agctccagaa
     1861 agacgagctg tcgctggaag agttcttgaa gaatgtccga ttctttgagg tcaaggagac
     1921 caaaccggaa cctcagccgg gtggcgaagg agtgccacct gggggacagt agttgagcac
     1981 aagacgtttc gactgaaacg ctcggtcaga tggagacact tgagagcgat tcagctgaag
     2041 ggcaattgag atggcaaaga tgacgatggc aacagtccgg aacctgttta cccttcatcg
     2101 ggacaggctt ctggctgctt tcctg
//
LOCUS       DAOQUC010000721         2121 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_65941_length_2121_cov_3.182962, whole genome shotgun sequence.
ACCESSION   DAOQUC010000721 DAOQUC010000000
VERSION     DAOQUC010000721.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2121
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65941_length_2121_cov_3.182962"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(289..735)
                     /locus_tag="PKH07_11860"
     CDS             complement(289..735)
                     /locus_tag="PKH07_11860"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNT35681.1"
                     /translation="MKEKSIKIRVAVIVQKENAVLMIRHQKAEKTYWLLPGGGLEFGE
                     TITGCAVRELMEETGLCVRPGNLLFVCESLPPDGHRHVLNLFLHAELLGGELALGDEE
                     ILAEARFVPMEEIPSLTIHPPIKSVLLDYLRSGKLPAEIMLGSMWD"
     gene            complement(735..2018)
                     /gene="hflX"
                     /locus_tag="PKH07_11865"
     CDS             complement(735..2018)
                     /gene="hflX"
                     /locus_tag="PKH07_11865"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HNT35682.1"
                     /translation="MFDSCVHAERALLVGAFIKGALSKDERELIMQEMLGLAETANLE
                     ILCCLQTVVQEPHSGTFLGQGKVDEISDMCQNLDPDVVVFQRDLSPVQQRNLERRLER
                     KVLNRTELILDIFAQHAHSRSGRLQVELAQLRYRLSHLTGRGVLMSRLGGGIGTRGPG
                     ETQLETDRRSIQYRITKLKEELGKLSKHRTEQRKSRSQSGLPVVSLVGYTNAGKSSLL
                     NALTESDVSAEDQLFTTLDPTTRKLPLQDGTQALLTDTVGFIRDMPKDLKIAFRATLE
                     EIKTSALILLVVEVNCPGMEQKIEAARKILEELGVMDRPVLTVFNKSDTLEPDSEVMD
                     WRVPHEPSCLVSAKTREGLSELVELIGSFVSSSRRQVLLRLPYSEGRIRSQIHEEGKV
                     ISESFGESCIDLECEIPLSLLPKVKPFVVTRERSG"
BASE COUNT          502 a          575 c          527 g          517 t
ORIGIN      
        1 aatcaagtat gtgtgaaggc gatttctcag gctgacttga tcggcctttc ttctgtttct
       61 ttgtgctcat tttcttccct aggatctgct tcttcgcgag acgttcgctc ggcgagaatg
      121 aaatgttcga ttgtaatctt tctgcggagc ctcaccatag gaataacgtc cggtcttgcc
      181 aatgaggcag acccattcca tctatggcag tctttcggat attacatcca actccaagtg
      241 agactctagg cgcatttgtc catgaaataa agggaaaaat cccaggattc agtcccacat
      301 tgagcccagc atgatctctg caggaagctt tccgctgcgg agatagtcca gaagcacgga
      361 tttgatgggc ggatgaatgg tcaaactcgg aatctcttcc atgggaacaa aacgggcttc
      421 ggcaagaatc tcttcgtctc cgagagccaa ttctccgccc aaaagctcgg catgcagaaa
      481 aaggttcaga acatgacgat gtccatccgg cggaagtgac tcgcagacaa acagcaggtt
      541 ccccggtcgc acacagagac cggtttcctc catgagttcg cgcaccgcac agccggtgat
      601 tgtctctccg aattccagtc cgcctccagg aaggagccaa taggtctttt ctgctttctg
      661 atggcgaatc atcagaacgg cattctcctt ctgaacgata acggcgactc gtatcttgat
      721 agatttttcc ttcatcaacc tgaccgctct ctggtgacga caaaaggttt cactttcgga
      781 agaagagaca ggggaatctc acattcaaga tcaatgcacg attcgccaaa ggactccgaa
      841 attaccttgc cctcttcgtg aatctgggaa cgaatacgac cttcactgta tggaagacgc
      901 aggagaactt gtcttctgga actggagaca aaactcccga tgagttccac caactctgac
      961 aacccctctc tcgtctttgc ggaaacaagg catgacggtt catggggaac acgccagtcc
     1021 atcacttcac tgtcaggttc gagcgtgtcc gacttgttga agaccgtcag cactgggcga
     1081 tccatgactc cgagttcctc gagaatcttt cgcgctgcct cgatcttctg ctccatcccg
     1141 ggacagttca cttccactac gagcagaatc agagcagatg tcttgatctc ttccagagtc
     1201 gcccgaaaag cgatcttgag atccttgggc atatcacgga tgaacccaac cgtatcggtc
     1261 agaagcgcct gcgttccatc ctgtagaggc agtttgcgtg tcgttggatc caatgtggtg
     1321 aaaagctgat cttcagcaga gacatctgac tccgtcaggg cgttcagaag cgatgacttt
     1381 cccgcgttgg tgtagccgac gagagacaca accggcagcc ccgattgtga tcgcgacttg
     1441 cgttgttccg tccggtgctt gctgagtttt cccaattcct ctttcagttt ggtgattcgg
     1501 tactggatgc ttcttcgatc cgtctccaac tgcgtttcac ccgggccacg ggtaccgatg
     1561 cctccgccga gccttgacat gagaacgccc cgacccgtga ggtgagaaag acggtaacga
     1621 agttgagcca gctccacttg aagacgtccc gaacgggaat gtgcatgctg ggcaaaaata
     1681 tccagaatca gttctgttcg attgagcacc ttgcgctcaa ggcgacgttc gagattcctc
     1741 tgttgaacgg gagataaatc gcgttggaag accacgacat ctggatcgag gttctgacac
     1801 atgtccgaga tttcatccac ttttccctgt ccgagaaaag tcccggaatg cggttcctgg
     1861 acaacggttt gaaggcagca aaggatttca agattggcgg tttccgccaa cccaagcatt
     1921 tcctgcatga tgagctcgcg ctcatctttt gaaagggcgc cttttatgaa agcgccaaca
     1981 agcaacgccc tttcagcatg cacacaactg tcaaacatgg atgccaatca gaagggtttc
     2041 cgcctgggtt gtcaagccga ctactcgggg cgcgagttct ggccgtgcga agagttccat
     2101 ctcacgcggg tttccgcaga c
//
LOCUS       DAOQUC010000722         2121 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_65954_length_2121_cov_2.668925, whole genome shotgun sequence.
ACCESSION   DAOQUC010000722 DAOQUC010000000
VERSION     DAOQUC010000722.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2121
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65954_length_2121_cov_2.668925"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1044)
                     /locus_tag="PKH07_11870"
     CDS             complement(<1..1044)
                     /locus_tag="PKH07_11870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35683.1"
                     /translation="MPARKKRCDKSKPIRLGIWGLGRGMSFFRSCQALNIQVVAGCDY
                     NEHMRKRFLEHCPNAYATTDSEQFLKQDMDAVLVATFCPAHAGDSIRCLDAGKHVLSE
                     VTAFHTMAEGVELVEAVERTGLVYNLAENYPFGKPMMYLSSRWKEGLFGDLMYAECEY
                     VHECRTLAYTYIDGVPIVPGNTVHNWRSWIHFHYYNTHSMGPVMIIGNARPTKVVSLP
                     GRVRLPGYLMKSERGMSGIAPSLISFENGVVMRNLMGATTNDTHIQRFWGTLGSAEFV
                     DGEMRFRLGASGHSPKLPVEVKWPEQGELADLAGHGGGDFWVLYYFARQIQTGEPAPF
                     DVYAAADCTIPGIL"
     gene            complement(1073..>2121)
                     /gene="ahcY"
                     /locus_tag="PKH07_11875"
     CDS             complement(1073..>2121)
                     /gene="ahcY"
                     /locus_tag="PKH07_11875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013537999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HNT35684.1"
                     /translation="TLCASNPLSTQDDVAAALVDKFEIPVFAVRGEDKNTYYDHINAV
                     LDQKPNITLDDGADLVSTLHSERTELLSSVCGSMEETTTGVVRLRSMERDGVLKFPVI
                     SVNDAMTKHMFDNRYGTGQSTVDGIIRATNKLFSGAIVVVAGYGWCGRGFAMRARGLG
                     AQIIVTEVDAIKGLEALMDGYRVMPMAEAVPLGDIFVTLTGDIHVLRKEHFEAMKDGA
                     IVCNSGHFNVEIDIPALEAMSSAPKEVRTNVQEYTLSDGRRICILGEGRLVNLAAAEG
                     HPACVMDMSFANQALCSEFVIKNQAKLEWRVYPVPKEIDQEVARLKLASMGILIDELT
                     PEQIKYLSSWEEGT"
BASE COUNT          510 a          628 c          523 g          460 t
ORIGIN      
        1 cagaattcca ggaatcgtgc agtccgcagc agcatagacg tcaaaaggag caggctcccc
       61 tgtctgaatc tggcgggcga agtagtagag cacccagaaa tcaccacccc catgacccgc
      121 aaggtctgcc agttcgccct gttcaggcca tttcacctct actggcaatt ttggtgaatg
      181 cccgctcgca cccagtcgga agcgcatttc cccatccaca aactcggcgg aaccgagagt
      241 cccccagaag cgctgaatgt gtgtgtcgtt ggtcgtcgct cccatcaagt tacgcatgac
      301 aacgccattc tcaaaagaaa taagcgacgg ggcgatgccg gacatccccc tctccgattt
      361 catcaaataa cctggtagtc gaacccgacc agggagagag acaacctttg tgggacgggc
      421 gttcccgatg atcatcaccg gccccatcga atgcgtgttg tagtaatgga agtgaatcca
      481 acttcgccag ttgtgaactg tgttgcccgg aacaatgggc accccgtcaa tgtaagtgta
      541 agcgagagtg cggcactcat ggacgtattc acactcggcg tacatcagat cgccaaacag
      601 cccttccttc catctgctgc tcagatacat catgggcttg ccaaagggat agttctccgc
      661 gaggttgtag acaaggccgg tgcgctccac ggcttctacc agttcgacac cttccgccat
      721 cgtatgaaac gcggtcactt cggacaagac gtgcttgcca gcatccagac agcggatcga
      781 atcgcccgca tgggcgggac agaacgttgc gacaagcacg gcgtccatat cctgcttgag
      841 aaactgctca gaatctgtag tcgcatacgc gttggggcag tgctccagga atcgcttccg
      901 catgtgctca ttgtagtcac atcctgcgac aacctggatg ttcagagcct ggcaagatct
      961 gaagaaactc atcccgcgcc ccaaacccca gatacccaat cggatcggct tactcttgtc
     1021 gcagcgcttc ttgcgtgcag gcacgatggc acctccacca atctgtgatg gcctaggtcc
     1081 cctcttccca ggaactcaga tacttgatct gctctggcgt caactcatca atcaagattc
     1141 ccatggaagc caatttcaga cgcgccactt cctggtcaat ctccttcgga acagggtaga
     1201 cacgccattc cagtttcgcc tggttcttga tcacaaattc agaacacaac gcctggttcg
     1261 caaaagacat atccataacg caagcgggat gtccctctgc agcagcaagg ttcaccagac
     1321 gcccttcacc aagaatacag attctgcgac cgtcagaaag cgtgtattcc tgcacgttgg
     1381 ttctgacttc cttcggtgca ctggacatgg cctccaaggc ggggatgtct atctcgacgt
     1441 tgaagtgacc ggaattgcag acaatggcac catctttcat cgcctcaaaa tgttccttgc
     1501 ggagcacatg aatgtcaccg gtaagcgtca caaagatatc tccaagcgga accgcttcag
     1561 ccatcggcat cacccgatag ccatccatga gcgcctcaag acctttgata gcatccactt
     1621 cggtaacgat aatctgtgcc cccaagcctc ttgctctcat ggcgaaaccg cggccgcacc
     1681 agccataacc ggccacaaca acaatggctc ctgagaagag tttgttcgtc gctcgaatga
     1741 tgccgtccac tgtgctctgc cccgttccgt aacggttgtc aaacatgtgc ttggtcatgg
     1801 catcattgac ggaaatgact ggaaacttga gaacgccatc gcgttccata ctgcgcagac
     1861 gaaccactcc cgtcgtggtc tcttccatgc ttccacagac ggaagaaaga agttccgttc
     1921 gctccgagtg cagcgtcgaa accaggtcgg cgccatcatc gagggtgata ttcggtttct
     1981 gatcaagcac ggcattgatg tgatcgtagt aggtgttctt gtcttctcca cggacggcaa
     2041 agacagggat ctcgaacttg tccacaagtg cggcggcaac atcatcctgg gtggaaagcg
     2101 gattggatgc gcacaaagtg a
//
LOCUS       DAOQUC010000723         2119 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_66041_length_2119_cov_3.307171, whole genome shotgun sequence.
ACCESSION   DAOQUC010000723 DAOQUC010000000
VERSION     DAOQUC010000723.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2119
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66041_length_2119_cov_3.307171"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /locus_tag="PKH07_11880"
     CDS             complement(<1..407)
                     /locus_tag="PKH07_11880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35685.1"
                     /translation="MGIAPPPADAGLPGRTHPTRFLVWFPVVACLIVAAAGLLFRSAR
                     LGDRPMHHDEAVQAVKTGVLFETGEYRYDPRDCHGPSLYYLTLPFLWLSGAQDFAQSN
                     ELIFRMVPVLFGVGLILLVLLVLDGLGWGACVWA"
     gene            complement(449..>2119)
                     /locus_tag="PKH07_11885"
     CDS             complement(449..>2119)
                     /locus_tag="PKH07_11885"
                     /inference="COORDINATES: protein motif:HMM:NF016563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH116 family glycosyl hydrolase"
                     /protein_id="HNT35686.1"
                     /translation="GLPPEDKSKCACAPPAEGELPPPQWRPFYASQWGDAREVALYVK
                     RYYRSLRDRTQAFHDALFSSTLPSYVLDAVSSNLAIIKSPTVLRQANGNMWAFEGCMC
                     DKGCCPGSCTHVWNYAQAVPHLFPQLERTLREQELVRSMDDRGHITFRAALPDGPTTH
                     QFHAASDGQLGGIMKLYRDWQISADRDWLERLYPPAKKSMNYCIEAWDPNRLGVLVEP
                     HHNTYDIEFWGPDGMCSSFYLAALAALSQMGRELGFTEEAEFYDDLSRRGAKYLDDHL
                     FNGEYYFQEVLYDKLRDKSFLDMISKVDAKSSEVMQLLKREGPKYQYGIGCISDGVIG
                     AWMAKIYGIDTPQNAEHVRRNLQAIFEYNFRDDLFEHANPQRPGYALGHEPGLLLCTW
                     PNGGRPTLPFVYSDEVWTGIEYQVASHLIEEGFVDEGLTLVRAVRQRYDGHVRNPWNE
                     YECGSYYARAMASYSLLGSLAGMRYSKVTKTLWFAPKLQRRSFRVFFSGADGYGVISL
                     DGDQLKIELTEGHLSLERVVVTRKGKTFEVRCKKVAKAGKPLSIPIRS"
BASE COUNT          511 a          602 c          545 g          461 t
ORIGIN      
        1 gcccagacgc acgcacccca acccagtcca tccagaacca agagaacgag caggatcaga
       61 ccaactccaa acaggacggg aaccatcctg aaaatcagtt cgttggactg agcaaaatcc
      121 tgtgctcctg aaagccacag gaaagggaga gtgagataat agagactggg gccatggcag
      181 tctcgaggat cgtaacggta ctcacccgtt tcaaagagga cgccggtctt gactgcctga
      241 accgcctcgt cgtggtgcat gggacggtcg cccaacctgg ccgaacgaaa cagcaggccg
      301 gcagccgcaa caataagaca cgctacgaca ggaaaccaga caagaaaccg cgtcggatgc
      361 gttcttcctg gcagaccagc gtctgcaggc gggggggcta tgcccactct tacagacgct
      421 tgctcattgg aagaagaatt ctcgtttcct aggagcgaat cggtattgat aggggtttgc
      481 ctgctttcgc taccttcttg catctgacct cgaacgtctt tcctttacgg gtgacaacaa
      541 ctcgctccag agacaagtgt ccttccgtca attcgatctt cagttgatcg ccgtcgagag
      601 agatcacgcc atatccgtcc gctccggaaa agaacaccct gaaactccgg cgctgaagtt
      661 tcggagcaaa ccagagggtc tttgttacct tggaataccg catgcccgca agcgagccta
      721 gcaacgaata acttgccatg gctctcgcgt agtagctgcc acactcatac tcattccaag
      781 gatttcggac gtgcccgtca tagcgctgtc gaactgcccg aacaagagtc aagccctcat
      841 ccacaaagcc ttcttcgatg agatgggacg ccacctgata ctcgataccg gtccagactt
      901 catctgagta gacaaaggga agtgtgggtc tgcccccgtt tggccaagtg catagaagca
      961 ggcctggctc atgaccaagt gcatatccag gcctctgcgg gttggcatgt tcgaagagat
     1021 cgtcccggaa attgtactca aaaatcgcct gtagatttct gcgcacgtgc tctgcatttt
     1081 gcggtgtatc gatcccgtat atcttcgcca tccatgcacc gatgactcca tccgaaatac
     1141 acccgatgcc gtattggtac ttggggcctt ctctcttgag cagttgcatg acttcgctgc
     1201 tcttggcatc tactttggag atcatgtcga gaaaagattt gtcgcgaagt ttgtcgtaga
     1261 gcacttcttg gaagtagtac tcaccgttga agagatgatc gtcaagatac tttgctccgc
     1321 gtcgcgaaag gtcatcatag aactcggcct cttcggtgaa tcccaattcc cttcccatct
     1381 gcgacaatgc agccagcgcc gcgagataga aactcgaaca catgccatct ggaccccaaa
     1441 actcaatgtc ataggtgtta tggtgtggct ccaccaagac tccgagccga ttcggatccc
     1501 aagcttcgat gcaatagttc atgctcttct ttgccggtgg atagaggcgt tcgagccagt
     1561 ctcggtcagc cgagatctgc cagtcccgat agagtttcat aatgccaccc aactgcccat
     1621 cggacgccgc atgaaactgg tgtgtcgtgg gaccatccgg cagcgctgcc ctgaaagtaa
     1681 tgtggcctcg gtcatccatg gaacgcacca gttcctgctc ccgcagggta cgctccagtt
     1741 gcgggaaaag atgaggaaca gcctgggcat agttccacac atgggtacag gagccgggac
     1801 agcatccttt gtcgcacata cagccttcga acgcccacat attcccattg gcttgccgaa
     1861 gcaccgtggg agacttgata attgccaggt ttgaggatac tgcgtcaagc acgtacgaag
     1921 gaagcgttga agagaacagc gcatcatgaa acgcctgtgt cctgtcgcgg agtgacctat
     1981 aataacgctt cacatacagc gccacttccc tggcatcacc ccattgggaa gcatagaaag
     2041 gtcgccactg gggtggtggc aattctcctt ccgccggtgg ggcacacgca cacttgctct
     2101 tgtcttcggg tggaagccc
//
LOCUS       DAOQUC010000724         2116 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_66208_length_2116_cov_3.335274, whole genome shotgun sequence.
ACCESSION   DAOQUC010000724 DAOQUC010000000
VERSION     DAOQUC010000724.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2116
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66208_length_2116_cov_3.335274"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..780
                     /locus_tag="PKH07_11890"
     CDS             <1..780
                     /locus_tag="PKH07_11890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35687.1"
                     /translation="DSDVLVCSLGSTGSTILVVDGYDRWNAQSGGVNHTFLEKFADAL
                     SGNGARYDSCVNEALTGGSISLSDYSIVMWMSGEETTESETLSYDEQILLQNYLKQGG
                     ALFISGAEIGWDLIARGGSLNNYSNGSDNDTAFHRNFLKAEFVSDDANTYSVTGVNAT
                     GFAGLSFSFDDGTRGTYNVRYPDVLATYGGSTNALYYGAGPNVAGIVYSGTFPDGTFP
                     GKLVYFGFPFETIYDVAARTNVMSSVLAYLTPTPVNLWQMY"
     gene            complement(901..1695)
                     /locus_tag="PKH07_11895"
     CDS             complement(901..1695)
                     /locus_tag="PKH07_11895"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNT35688.1"
                     /translation="MKIVDFHTHAFPDSLAPVAMGRLADGSHEYAPHHDGTIASLLRS
                     MDESGIWSSVICSIATKPSQERPILEWSRQIVSDRIFPFISLHPEGTDHNALLDEARN
                     AGIRGAKFHCMYQGFKADDEHALPIFEKLAARNMLALFHAGRDIRWPKSDVASPERLL
                     NLHRRVPELCLVTAHTGGWLLWDEVEALLAGTQVIFDTSFTLGHITPEAWSRIWAKHS
                     HDRIVFGSDSPWASQKAALDDLRQAIPDDLEFEKVVSLNARRILGA"
     gene            complement(1729..>2116)
                     /locus_tag="PKH07_11900"
     CDS             complement(1729..>2116)
                     /locus_tag="PKH07_11900"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNT35689.1"
                     /translation="LERAVATCPQTVFIGHAPGFWAHISGDGQQTKASYPKGPIVPGG
                     KLPEMLRTYPNLYCDISAGSGHNALSRDLEFTKDFLAEFQDRILYGRDFFDNAHQQLL
                     NSLGLSQPTLDKIYSGNALRLLSAVS"
BASE COUNT          489 a          544 c          588 g          495 t
ORIGIN      
        1 gactccgacg tgctcgtgtg ttcgctgggc agtactggaa gcacaatcct cgtcgtggat
       61 ggttacgatc gctggaacgc tcagtccggt ggagtcaacc atacgtttct ggagaaattc
      121 gcagatgctc tttcaggaaa tggtgctcga tatgattcct gtgtgaacga ggcgttgacc
      181 ggcggctcca tctcgctgtc ggactattcc atcgtgatgt ggatgagtgg cgaggagaca
      241 accgaaagcg aaacgctcag ctatgacgaa cagattctct tgcagaatta tctgaagcaa
      301 ggtggtgcgc tcttcatcag cggtgcggaa attggttggg atctgattgc gcggggcggt
      361 tctttgaaca actactccaa cggcagcgac aatgataccg catttcaccg caactttctc
      421 aaagccgagt tcgtcagcga tgacgcgaac acttactcgg tcacaggggt gaatgcaaca
      481 ggctttgcgg gtttgtcgtt ttctttcgac gatggaaccc gcggaaccta taacgtcagg
      541 tatcccgatg tgctcgccac ctatggtgga agcacaaatg cactctacta tggcgcggga
      601 ccaaacgtcg cgggcattgt gtattcaggc actttccccg atggaacgtt tccaggcaaa
      661 ctcgtctatt ttggatttcc attcgagacc atctacgatg ttgccgctcg aacaaacgtg
      721 atgagtagtg tgcttgccta tttgacacca acgccggtca atctttggca gatgtactga
      781 tggcctgatc tgagaaaaac agtgcatctt acagatgcag gatagtggtg ggactgtgct
      841 tcttgcgttc gatcgctcgc ggtgcggaat gatgcaagaa caggatttcc tgctataggc
      901 tcaggcaccc agaatccttc gtgcattgag tgagacaact ttctcgaatt cgagatcgtc
      961 gggaatggct tgcctcaggt cgtcgagggc cgccttctgc gatgcccatg gggagtcaga
     1021 accaaagaca attcggtcgt gcgaatgctt tgcccagatc ctgctccacg cttcgggagt
     1081 gatatgcccc aaagtgaagg acgtgtcgaa gatcacttga gtgcccgcca gcagcgcttc
     1141 cacttcgtcc cataggagcc atccccctgt atgcgccgta acaagacaga gttcaggaac
     1201 ccggcggtgc agattcaaga gtcgctcggg tgaagccaca tcactcttgg gccatcgaat
     1261 gtcgcggcca gcgtgaaaga gtgccagcat gtttcgcgcg gccaactttt caaagatggg
     1321 caaggcgtgt tcatcgtcgg ccttgaagcc ctgatacata cagtgaaact tggcacctcg
     1381 aataccggcg ttccgtgctt catccagcaa cgcattatga tcggttcctt cgggatgaag
     1441 cgagatgaaa gggaagattc ggtcggaaac aatctgtctt gaccactcaa ggatggggcg
     1501 ttcctgactc ggttttgtcg cgatggaaca aatgaccgaa ctccagatgc cggattcgtc
     1561 catcgatcgc agaagagagg cgattgttcc gtcatggtgt ggtgcgtact cgtgagatcc
     1621 gtcggcaagc cgacccatcg ccaccggcgc gagggaatcg gggaaagcgt gtgtgtggaa
     1681 atctactatt ttcatcttcc agcgctcatc cgctcaagtg cctcaacctc acgaaaccgc
     1741 tgacagcaat ctcagcgcat tgcccgaata aatcttgtcc aaagtcggct gggagagacc
     1801 gagcgagttc agcagttgct gatgtgcgtt gtcgaagaag tctcggccat agagaatcct
     1861 gtcctgaaac tcggccaaaa aatccttcgt aaattcgagg tcgcggctca gagcattgtg
     1921 tcctgatccg gcagaaatat cgcagtaaag atttggatac gtccgcaaca tctccggcaa
     1981 ctttccacct ggaacaatcg gtcctttcgg gtaggatgct ttggtctgct gtccgtctcc
     2041 ggaaatgtgc gcccaaaagc cgggtgcatg accgatgaac acggtctgtg ggcaagtggc
     2101 aacagcacgt tccaga
//
LOCUS       DAOQUC010000725         2115 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_66255_length_2115_cov_3.178641, whole genome shotgun sequence.
ACCESSION   DAOQUC010000725 DAOQUC010000000
VERSION     DAOQUC010000725.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2115
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66255_length_2115_cov_3.178641"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            120..1394
                     /locus_tag="PKH07_11905"
     CDS             120..1394
                     /locus_tag="PKH07_11905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011399157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35690.1"
                     /translation="MGLSTDSSVAEDSHPQSRHSAFSIPDQLSGFWEARGGVRTRSDP
                     HEKQSSIGETRLQLEWEERLRLGVLKLTTDFLYDPVMSDHQMRLERGKGWLDIRNASF
                     QTRPLDFVDVKVGRQILTWGTGDMLFINDLFPKDWNSFFIGRDDEYLKAPSDAIKTSL
                     FSRWANLDIVYTPRFDGDRFIDGRRISYYNAMLGRRAGRDAPVHLDRPYDWFHDDELA
                     LRLSRNVFGYELALYAYDGFWKSPGGLDPVTFEAVCPELSVYGASVRGTVGRGIGNME
                     IGYYDSREDRRGDDPFTRNSEFRFLAGYEQEVARDLTAGVQYYVEWMMDYDQYMASLP
                     PGTHAADEDRHVLTLRLTKLLLNQNLNVSLFTYYSPSDSDVYFRPKVHYKVDDHWSAE
                     VGGNVFVGEEDHTFFGQFEKNSNVYVAARYSF"
     gene            1567..1728
                     /locus_tag="PKH07_11910"
     CDS             1567..1728
                     /locus_tag="PKH07_11910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011967814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HNT35691.1"
                     /translation="MKKYVCTVCGYVYDPEEGDPGNGVAPGTSFEAIPDDWTCPLCGA
                     PKSDFEAEE"
     gene            1899..>2115
                     /locus_tag="PKH07_11915"
     CDS             1899..>2115
                     /locus_tag="PKH07_11915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35692.1"
                     /translation="MAQDGIVSSTSTKSNLQLEALLNETQKLSKVGGWEWDVTRQVMT
                     WTNETFRIHGLPPSKARNVSADLIALSV"
BASE COUNT          475 a          531 c          600 g          509 t
ORIGIN      
        1 cttccaccgg gagagaatca tctgagcctg atcttcccat cggactggga acgcaacgtt
       61 ccgacgagcc atcacttccg acgggtctgg atcccaaacc caaagaaccg gagttgcctt
      121 tggggctgtc aacggattcc tctgtcgctg aggactccca ccctcaatcg aggcattctg
      181 ctttttcgat tccggatcaa ctgagcggtt tctgggaagc acgtggcggg gttcgaacac
      241 ggagcgaccc ccatgagaag cagtcttcca ttggggagac acgcctgcag ttggagtggg
      301 aggagcgtct gagactgggt gtgctcaaac tgacaacgga cttcctgtac gatcccgtta
      361 tgagcgatca ccagatgaga ctcgaacgag gtaagggatg gctggacatc agaaatgcca
      421 gtttccaaac acgacccctg gactttgtgg acgtcaaagt tggcagacag attctgactt
      481 ggggcacagg ggatatgctc ttcatcaatg acctttttcc caaggactgg aactcatttt
      541 tcatcggtcg tgacgatgag tacctgaagg ctccctccga tgcgatcaag acgtccctgt
      601 tctccaggtg ggcgaatctg gacattgtct acacgccgcg gttcgatggt gatcggttta
      661 ttgatggtcg ccgcatctcc tactacaacg ccatgctggg tagacgcgcc ggacgggatg
      721 cgccagtgca tctcgatcgt ccatatgact ggtttcacga tgatgagctt gccctgcgtc
      781 tttctcgaaa cgtttttggt tacgaattgg cgctctatgc ctatgatggt ttctggaaaa
      841 gtccaggggg gctggatccg gttacctttg aggcggtctg ccccgagctt tcagtctatg
      901 gagccagcgt tcgtggtacc gtcgggcggg gaatcggaaa catggagatt ggctactatg
      961 attcgagaga agatcgccgt ggcgatgacc cgttcacccg caacagcgaa tttcgttttc
     1021 tggctggcta cgaacaggaa gtagccaggg atttgactgc gggcgttcag tactacgtcg
     1081 agtggatgat ggattacgat cagtatatgg cttctctgcc gcccggaacc catgctgcag
     1141 atgaagatcg gcatgtgctg acgctgcgct tgacaaaact ccttctgaat cagaacctga
     1201 atgtgtccct cttcacctat tattctccca gtgacagtga tgtctacttc cgacccaagg
     1261 ttcactacaa ggtggacgat cactggtctg cggaagttgg tggaaatgtc tttgttggtg
     1321 aagaagacca taccttcttc ggtcagtttg agaaaaacag caatgtctat gtggcagctc
     1381 gctacagttt ttagcggttt gcacactttg ctgaagtgct gagcaggttc acacctgcag
     1441 gcgggtgggt catgcccact ccatcggatg cttgtcctgt aacggacagg cctctcctga
     1501 aatcagtgtc acgaggtgaa caatcgtctc cgtgagagcg aatactaagt aggaggaatg
     1561 ggaaccatga agaagtacgt ttgcacggtc tgtggctatg tttacgatcc ggaggaggga
     1621 gacccgggaa acggagttgc tccaggcact tccttcgagg cgattccgga cgactggact
     1681 tgccccctgt gtggtgcacc caagtcagat tttgaagcgg aagaatagtc accctgaggg
     1741 cactcattga cagagaaccg tggtgtgtgc ggagcgcgtg agcactctcc acctcttact
     1801 gatttgaacc tgataccact cactttcgaa tccgtttgca cgcggtcgaa taatgaacta
     1861 taagggcacc atcaccgcgg tggattaggg acatcggcat ggcacaggat gggatcgttt
     1921 caagcacttc aacgaagtca aatcttcagc tggaagccct gctgaatgaa acccaaaagc
     1981 tcagtaaggt gggtggctgg gagtgggacg tcactcgaca ggtcatgaca tggacgaatg
     2041 agacttttcg catccacggt ctgcctcctt cgaaagcgcg gaatgtctcg gccgatctga
     2101 ttgctctgag cgttg
//
LOCUS       DAOQUC010000726         2111 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_66432_length_2111_cov_4.637646, whole genome shotgun sequence.
ACCESSION   DAOQUC010000726 DAOQUC010000000
VERSION     DAOQUC010000726.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66432_length_2111_cov_4.637646"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..298)
                     /locus_tag="PKH07_11920"
     CDS             complement(<1..298)
                     /locus_tag="PKH07_11920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35693.1"
                     /translation="MRKSVRVKVPCSTSNLGPGLDCLGLALSLYFELTVSLDRRADSD
                     FQWQGETLEPKDQDAARQLLLRSMGVVFQAVGREMPPLRMTVRSGIVDVLKRLGY"
     gene            complement(295..1632)
                     /locus_tag="PKH07_11925"
     CDS             complement(295..1632)
                     /locus_tag="PKH07_11925"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HNT35694.1"
                     /translation="MSEKSDVQQKLELSELVSLCKRRGFIFQSSELYGGLNSCWDYGP
                     LGVELKRRVKDIWWRDTVQFRNDVVGIDASILMHPRVWEASGHVESFHDPMVDCKECK
                     GRFRADHLEGDKCPECGGELTEPRDFNLMFKTHVGAVADDASAVYLRPETAQAIFVDF
                     EQVMTCSRLRIPCGIAQIGKSFRNEVTTRYFIFRSREFEQMELEFFVAPDDEERWFEY
                     WLDQRLNWYTSSLGIKKGSLRLRPHEKTELAHYAKGCSDVEFLFPFGWSELEGIANRT
                     NFDLSQHAKFSGKKIEYFDDLSGQKFVPSVIESSAGVDRTILTILMDAYDVEPERTVL
                     RFHPDVAPIQVAVFPLMKKPELVEVATKLEKDLRKQFRTFYDVTGNIGRRYRRQDEIG
                     TPYCVTIDYDSLNDHSATLRDRDSMKQKRVSFDQMIAAIGEAQSNPDFWSQSQ"
     gene            complement(1703..2002)
                     /locus_tag="PKH07_11930"
     CDS             complement(1703..2002)
                     /locus_tag="PKH07_11930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35695.1"
                     /translation="MRKRYWIIIVIALIVVGCLAAAYRFHPPTHRWITSNVIERYRVT
                     EEPERTFDDVKAEIDREKAARIARFKKDEEAKQQEIERLKAEKAKLEKQLQDLRK"
BASE COUNT          462 a          611 c          520 g          518 t
ORIGIN      
        1 cgtaaccaag ccgctttaaa acatcgacga ttccacttcg aacggtcatt cgaagcggcg
       61 gcatctcccg acccaccgcc tgaaaaacaa ctcccatgga gcgcagaagc aactgacgcg
      121 cggcatcctg atccttcggc tccagtgtct cgccttgcca ttgaaaatcc gaatccgctc
      181 tgcggtcaag gctgactgtc agttcgaagt acagagagag agccaagccc agacagtcca
      241 gtccaggtcc cagattggaa gtcgaacagg gcactttgac gcggacggat tttctcattg
      301 tgattgcgac cagaaatctg gatttgactg tgcctctccg atcgcggcaa tcatctgatc
      361 aaacgaaaca cgcttctgtt tcatggagtc ccgatcgcgc agcgtggcgc tgtggtcgtt
      421 tagactgtca tagtcaatgg tgacacagta gggagttccg atttcatcct ggcggcggta
      481 ccggcgtccg atgtttccgg tcacgtcata gaacgttcgg aactgttttc ggagatcttt
      541 ctcaagtttg gtggccactt ccaccaattc gggttttttc atgaggggaa atacggcgac
      601 ctgaatcgga gccacgtcgg gatgaaatct cagaactgtc cgttccggtt ccacatcgta
      661 ggcgtccatg agtatcgtca gaatggttcg atccacacca gcggatgatt caatgacgga
      721 aggcacgaat ttctgtcctg agagatcgtc gaagtattcg atcttcttgc cgctgaactt
      781 ggcatgttga gacagatcga aattggttcg gtttgcgatg ccttcgagct cagaccagcc
      841 aaacgggaaa aggaattcca catcgctgca gcctttggca tagtgcgcca gctcggtctt
      901 ttcatgaggt ctgagtcgaa gactgccttt cttgattccc agcgacgagg tgtaccagtt
      961 caaacgctga tcgagccagt attcaaacca acgctcctca tcgtccgggg ccacaaagaa
     1021 ctccagttcc atctgttcga attccctcga acggaagatg aaataccgtg tggtgacttc
     1081 atttcgaaag gactttccaa tctgagcgat accacagggg atgcgcagtc ggctgcatgt
     1141 catcacctgc tcgaagtcca caaagatcgc ctgcgccgtt tctggtctca ggtagacagc
     1201 gctggcatcg tcggcaaccg caccgacgtg cgtcttgaac atcaggttga aatcacgcgg
     1261 ttcggtcaac tcgccaccgc actcgggaca cttgtcccct tccagatggt cggctcgaaa
     1321 acgccccttg cattccttgc agtccaccat cggatcatgg aacgactcta catgtccgga
     1381 cgcttcccag acacgcgggt gcattagaat actggcgtca ataccaacca cgtcgttgcg
     1441 aaattgaacc gtgtcccgcc accagatgtc cttgacgcga cgctttagct ccacacccaa
     1501 aggaccatag tcccagcagg agttcaaccc tccatacagt tcactgctct ggaaaataaa
     1561 cccgcggcgc ttgcacagtg agaccagctc actgagttcc aatttctgct gcacatcact
     1621 cttctcgctc actctcgttc ctttctctgt ctctgacggg ggaaccggct tgcggtttgc
     1681 gtccgtcgtt ccccgacaac aactacttgc ggagatcctg cagttgcttc tccagcttgg
     1741 ctttttccgc tttcaggcgt tcgatctcct gctgtttggc ctcttcgtcc ttcttgaatc
     1801 gggcgattcg ggctgccttc tcacgatcga tctcagcctt cacgtcatca aaagtccgct
     1861 caggctcttc tgtcacgcgg tagcgctcga tgacgttgct ggtgatccat cggtgcgtgg
     1921 gaggatgaaa gcggtaggcc gcagccagac aacccaccac gatcaaggca atgacaatga
     1981 tgatccaata gcgttttctc attgttttct cgagctttgg agcccgatcc cttcagataa
     2041 cgtgaggata cttgtaccct tagcgtttga gttctgtcaa gcctttgcta ccacatccgt
     2101 tcgaccgcat c
//
LOCUS       DAOQUC010000727         2108 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_66583_length_2108_cov_5.361422, whole genome shotgun sequence.
ACCESSION   DAOQUC010000727 DAOQUC010000000
VERSION     DAOQUC010000727.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2108
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66583_length_2108_cov_5.361422"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            201..1457
                     /locus_tag="PKH07_11935"
     CDS             201..1457
                     /locus_tag="PKH07_11935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35696.1"
                     /translation="MFRSGLIPVLFFLLWFCALLLSYGCTSGGNAEVGQERHLTRENT
                     AELFDKPLDRMQVALLDLAFECASKIPVYPHIKDRSKTQELVVQTCLELDQPKRALAY
                     MERIDDWRRGACRADLAYYLAEKGLMSLVQQHLEVAERIAEATDDWRRDTIRVKIAKT
                     HALIGNREKASEFEEGVVESETGKVVAVLAGKESEDTFTEQANQLESLVSTGSFDLVK
                     NALEGWTQIFDHYYHDVPKRERAERAVKNSWEKIPIFIRVELLTAMAKTALEHQDPAK
                     ALSLADEAQQFVESHQWRLEHRIPLVAPLITIWHQAGDHEKACQTAETLRSLYDQEST
                     GIVNIYRAGALRPLAESYALVGDATSAVSLYARAVEEGIANPNSRPRAEDLSATCASM
                     AKCTVAPSANLWTRIREIYEGLGDPW"
     gene            1451..>2108
                     /locus_tag="PKH07_11940"
     CDS             1451..>2108
                     /locus_tag="PKH07_11940"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HNT35697.1"
                     /translation="MVGRSHRFMLLCALLWAPVSVCAQQVGSIRGVVYDKDFETPLAS
                     ATVTVAEKDEKTTATEEGNYVFPQLPAGTYTLVFTKDGYTPQVKPNVLVVAGQMTEVD
                     MWLSGDFVEMEEFIVQDIQFESGTESALLELRLESPSLMNSISADLISQAGASDAASA
                     LQLVAGATIQEGKFAVVRGLPDRYVNSQMNGVRLPTADPDKRAVHLDQFPSTIIESIQ
                     V"
BASE COUNT          524 a          524 c          582 g          478 t
ORIGIN      
        1 tcccgcctcg gcctgtgctt tcgaatacac gactaacacg atagcaacag gctcctagct
       61 ctgctctaac aattcggtac taagaaacaa gcctcagttg gaacgtgcca ttgcgatggt
      121 gtcgtgaggc cagtagtctg gaaagaggat cggatgaggg atgctctgtg cgtctgaaag
      181 agaatctgag gaacacaacc atgtttcgat caggacttat ccctgttttg ttcttcctgc
      241 tctggttctg tgcgctgctg ctttcgtacg gctgcacgag tggtggaaac gccgaagttg
      301 gacaagagcg tcatttgacc cgagagaaca cggcagaact ttttgacaag cccttggata
      361 ggatgcaagt cgctctgctt gatctggcgt ttgaatgtgc cagcaagatt cctgtttatc
      421 cacacatcaa ggatcgctca aagacacagg aactggtggt acaaacctgc ctcgaacttg
      481 atcagccaaa gcgagctctt gcctatatgg aacgaattga cgactggcgg cgtggtgcct
      541 gtcgcgccga ccttgcctat taccttgccg aaaaaggttt gatgtctctt gtacagcagc
      601 acctggaggt tgctgagagg attgccgaag caaccgacga ctggcgaaga gacacaattc
      661 gcgtcaagat cgccaagact catgctttga ttggaaatcg ggaaaaggcg agtgaattcg
      721 aagaaggggt tgttgaatcc gagacgggca aagtcgtggc agtcttggct gggaaggaat
      781 ccgaagacac cttcaccgag caagccaacc aactcgaatc tcttgtttcc acaggcagtt
      841 ttgacctggt caagaatgct cttgaggggt ggacacagat tttcgaccac tattaccacg
      901 atgttccaaa acgtgaacgg gcagaaagag cagtcaagaa ttcgtgggag aagatcccga
      961 ttttcattcg agttgaactg ttgactgcga tggcgaaaac agcccttgag caccaggatc
     1021 cagcgaaggc gctgtccctg gcggatgaag cacaacagtt cgtagaaagc catcagtggc
     1081 gtcttgaaca tcgcatccct ctggtggcac cgttgatcac aatttggcat caggcgggtg
     1141 atcatgagaa agcatgtcag acggcagaga cgctgcgttc actctatgat caggagagta
     1201 ccggtattgt gaacatttac cgtgcaggtg cactgcgccc actggcagag agctatgccc
     1261 tcgttgggga cgcaacgtca gcagtttccc tctacgcaag agctgtagag gagggcattg
     1321 cgaatcccaa cagtcgccca cgcgctgaag atctgtccgc aacgtgtgct tcgatggcca
     1381 agtgcactgt ggcacccagc gctaatctgt ggactcgaat cagggaaatc tacgaaggac
     1441 ttggtgatcc gtggtaggac gatcacaccg attcatgctc ttgtgtgctt tgttgtgggc
     1501 acctgtcagt gtgtgcgctc aacaggtcgg cagcattcga ggcgttgtct atgacaagga
     1561 ttttgaaaca ccgctggctt ctgcaaccgt gacggttgcc gaaaaagacg aaaagaccac
     1621 tgcaacggaa gaaggcaact acgttttccc gcaactgccc gctggtacct acacactggt
     1681 cttcaccaaa gatggttaca ctcctcaggt caagcccaac gttctggtgg tcgctggcca
     1741 gatgaccgaa gtggacatgt ggctaagtgg cgattttgtg gagatggaag agttcatcgt
     1801 ccaggatatt cagtttgagt ccggtaccga atcagcactt ttggaactac gactggaaag
     1861 cccttcactg atgaactcca tcagtgccga tctgatcagt caagcaggcg cgagtgacgc
     1921 agccagtgct ctacagctgg tggcgggagc aaccattcag gaaggtaagt ttgccgtggt
     1981 gcggggtctt ccagaccgat atgtcaattc acaaatgaac ggggtgcgcc tcccgacagc
     2041 cgatcccgac aagcgtgccg tgcatcttga tcagtttcca tccacgatta tcgaaagcat
     2101 tcaagtca
//
LOCUS       DAOQUC010000728         2101 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_66963_length_2101_cov_2.701369, whole genome shotgun sequence.
ACCESSION   DAOQUC010000728 DAOQUC010000000
VERSION     DAOQUC010000728.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66963_length_2101_cov_2.701369"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            138..362
                     /locus_tag="PKH07_11945"
     CDS             138..362
                     /locus_tag="PKH07_11945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020505027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module protein"
                     /protein_id="HNT35698.1"
                     /translation="MNRKTPSVFDLTPSEKLQLVEDLWDDLASSPSDIPVADWQKKEL
                     DRRKANLMRCPVASSAWEDVKIRVRSHYGR"
     gene            352..651
                     /locus_tag="PKH07_11950"
     CDS             352..651
                     /locus_tag="PKH07_11950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012663335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HNT35699.1"
                     /translation="MAAELLIAPEAEQDIVDAYAWYEDRRHGLGEEFLCCLDACIEGI
                     LRNPEMHEKVYEDYRRGFVRRFPYAVFYEYNNATVTIYSVFHSSRNPEKWRQRLL"
     gene            complement(699..998)
                     /gene="nadS"
                     /locus_tag="PKH07_11955"
     CDS             complement(699..998)
                     /gene="nadS"
                     /locus_tag="PKH07_11955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="HNT35700.1"
                     /translation="MKEEMFEKLVMSLEESREIRHGRRKASRISEISAPNIKMVRRKL
                     KVSQAEFAMMVGVSARTLQNWEQGRRQPVGPAKALLRVAVMNPEAVLQALHGKQQ"
     gene            complement(995..1309)
                     /locus_tag="PKH07_11960"
     CDS             complement(995..1309)
                     /locus_tag="PKH07_11960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004822216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HNT35701.1"
                     /translation="MVFIETSVFTKEIKRLLTDDLYRTLQTALMIRPDAGEIIKGSGG
                     LRKIRWSRPGHGKRGSLRVIYYWDRPDTIYMLLPYCKSRQDDLTPAQLKSLRALMKEF
                     LS"
     gene            complement(1540..>2101)
                     /locus_tag="PKH07_11965"
     CDS             complement(1540..>2101)
                     /locus_tag="PKH07_11965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35702.1"
                     /translation="NLFGPELATTAGTQGPRGAAGGTAGLNITDSILYNECSGGAKNG
                     LAMIVNTKEAADADAFARCTFYSKPTMYYVVGTNSGTVPFRDCIFAGSGQVFPTAPVA
                     GLTLDVDYCAYVEAGAHAIGGRGGAQTDGANVISGDPLFLGINALLANAFDVDAASFG
                     GKGTAGSNLSGGADFIGSAVGSWDLY"
BASE COUNT          484 a          555 c          538 g          524 t
ORIGIN      
        1 gtgcgtccgc ttggctcggc gcgccacatt gctgtcacgc agttgacaag gtgctttgtc
       61 ttgacgtatg tagatattgt tttcagttgc gtgttgacat gcagttcgcc aatgcacacc
      121 aaggagtgga agtttccatg aatcgaaaaa cgccttcagt attcgacctc actccatccg
      181 aaaaacttca gttggttgaa gatctttggg atgacttggc atccagtccg tcagatattc
      241 cagttgcgga ctggcagaag aaggaattgg accgtcgcaa ggcaaacttg atgagatgtc
      301 ctgtcgccag ttctgcctgg gaggatgtca agattcgggt gcgaagtcac tatggccgct
      361 gaactgctca ttgcacctga ggccgagcag gatattgttg atgcatacgc ctggtatgaa
      421 gaccggcgcc acggtcttgg tgaggaattc ctgtgctgcc ttgatgcgtg catcgaaggg
      481 attcttcgaa atcctgagat gcatgaaaaa gtctatgaag actatcgaag aggttttgtt
      541 cgacgatttc cgtatgcggt cttctacgag tacaataacg caacagtcac catatacagt
      601 gtctttcaca gttcaagaaa tccggaaaag tggcgtcaac gattgctctg actcatctgg
      661 tgcgtccgcg ctgtgcttga cgcgccttga tactcctcct actgctgttt cccgtgcaat
      721 gcttgaagga ctgcctctgg attcatcact gcgacacgca gaagtgcctt ggcgggaccg
      781 acaggttgcc tcctgccctg ttcccagttc tgaagggttc ttgcgctgac tccgaccatc
      841 atggcgaact ctgcctgtga gactttgagc ttcctgcgaa ccatctttat gtttggcgca
      901 ctgatctcag atattctact ggccttcctt ctgccgtgtc ttatctcacg agattcctcc
      961 aaactcatca caagtttttc aaacatttct tccttcatga tagaaactcc ttcatcaatg
     1021 ctcgaagaga tttcagctgc gccggtgtca ggtcatcttg tcgtgacttg cagtatggca
     1081 agagcatata gatggtgtcc ggcctatccc agtagtatat cactcgaaga gaacctcgct
     1141 tgccgtgtcc aggccgactc cacctgattt ttcggagacc gccacttcct ttgatgattt
     1201 ctccggcgtc gggtcttatc atcaatgccg tttgcagcgt ccggtataga tcgtccgtta
     1261 agagacgctt gatctccttg gtgaaaaccg atgtctcgat aaagaccatg attctcttat
     1321 acgccaatgg cgtataatgt caacccctgg gactcataat ccccgcttaa taccccctgc
     1381 cttattaagg ggggattgag gggggatttg tatctgcacc aaaaagaaaa gacccgcgga
     1441 agggaaacct tccgcgggtc ttgaaattca tgaaaaccaa gctccgtctg tagcctgggt
     1501 tgttctcaac ccaggatcta cactgacacg aaggtcagat cagtacagat cccagctgcc
     1561 aacggcagaa ccgatgaaat cggcaccacc agacaggttg gagccagcag ttcccttgcc
     1621 accaaatgac gccgcatcaa cgtcaaaggc gttggcgagt agcgcgttaa tgccaaggaa
     1681 cagcggatcg ccgctgatga cattcgcgcc atccgtctga gcgccgcctc tgccaccaat
     1741 ggcatgcgca ccggcttcaa cgtaagcgca gtagtcaaca tcaagagtca gaccagcaac
     1801 aggagcggtc ggaaatacct gtccggagcc tgcgaagatg cagtcacgga agggaaccgt
     1861 gccgctgttt gtgccgacga cgtagtacat tgttggcttg ctgtagaagg tgcaacgtgc
     1921 aaatgcatcg gcatcagccg cttccttggt attgacgatc atcgcaaggc cgttcttggc
     1981 gccaccagag cattcgttgt agagaatact gtcggtgatg ttcaagccag ccgtgccacc
     2041 agcagcacct cggggaccct gagttccggc agttgttgcc aactctgggc caaagaggtt
     2101 g
//
LOCUS       DAOQUC010000729         2096 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_67196_length_2096_cov_2.571289, whole genome shotgun sequence.
ACCESSION   DAOQUC010000729 DAOQUC010000000
VERSION     DAOQUC010000729.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2096
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67196_length_2096_cov_2.571289"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..347)
                     /locus_tag="PKH07_11970"
     CDS             complement(<1..347)
                     /locus_tag="PKH07_11970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNT35703.1"
                     /translation="MGGKFILDTNIVIALFAGDKAVKDKVTQAEEIYVPIIVLGELHY
                     GAKKSSQVAANVARIEEFASSTSVLMCDRTTARFYGEVKEGLRRKGTPIPENDIWIAA
                     IARQHEMSLVARD"
     gene            complement(337..564)
                     /locus_tag="PKH07_11975"
     CDS             complement(337..564)
                     /locus_tag="PKH07_11975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006198662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2281 domain-containing protein"
                     /protein_id="HNT35704.1"
                     /translation="MEATITARVLEQIESLPEQLQQQVLDFAQALHLLAQKGTQGKQL
                     LRFAGCIPTDELKRMSEAIETGCEQVDKNGW"
     gene            complement(967..1042)
                     /locus_tag="PKH07_11980"
     tRNA            complement(967..1042)
                     /locus_tag="PKH07_11980"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1007..1009),aa:Gly,seq:tcc)
     gene            complement(1133..>2096)
                     /locus_tag="PKH07_11985"
     CDS             complement(1133..>2096)
                     /locus_tag="PKH07_11985"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNT35705.1"
                     /translation="ITSLITQSDAIHTEITESEASLQNVMTARDEVARQLRTLNDTHA
                     IHNTTKNDFEKALLRVEMEYKNLEERIQQEFEKPLDEVIQAAPNDERPTEVLMESLAE
                     VRRKIGQLGEVNPLAIQEFEEVEQRLNFLHEQHTDLEKARASLLRTIETLQKTASEKF
                     HEALNTIRENFRDVFRKMFRGGKADLVFIDLENNPDGGIEIVVQPPGKQLQNINLLSG
                     GEKALSAIALLFAMYLTKPSPFCLFDEVDAPLDDQNIGNFCRMIQEFSERSQFLIITH
                     NKRTMEMASTIYGVTMETKGISKILSLQMERQAPQDEEKISAAD"
BASE COUNT          452 a          554 c          539 g          551 t
ORIGIN      
        1 atatctcgag ccaccagaga catctcatgt tgacgagcga tcgccgcgat ccagatgtca
       61 ttttcaggga taggtgttcc cttccgcctg agaccttcct tgacttctcc gtaaaagcga
      121 gcggttgttc tgtcgcacat cagaacagat gtgctggaag cgaactcttc gatccgagcc
      181 acatttgcgg caacctgcga cgacttcttt gcgccgtaat ggagttctcc gagaacaata
      241 attgggacgt atatctcttc cgcttgggta actttatctt tcacggcttt gtcacccgca
      301 aagagtgcaa tcacgatgtt ggtgtccaga atgaacttac cacccatttt tgtccacctg
      361 ttcgcaccct gtctcaatag cctcgctcat gcgtttcagc tcatcagtgg gaatacaacc
      421 agcaaatcta agcaactgct ttccctgcgt gcctttctgt gcgagaaggt gcaaagcctg
      481 tgcgaagtcc aaaacctgtt gctgcaactg ctccgggagt gactctatct gctcaaggac
      541 tcgtgcggtt attgtggctt ccatatctca cctcaaattc cgaaccagaa acaaaccagc
      601 caagtttgaa ttctgaacag gccggcgcct gctggtttgc gggctatgcc cactctcttg
      661 gactcttgct cggtacatga cacgcctcac tcggactcag tgtcactagg agtatatcaa
      721 tgtccagagt tgttgccaac cgagctggct gatctttgaa gaacatgccc gatgcgctgg
      781 acaaggatgc gttcgcttct tatcagggtc tgcttcgctt cacttctgga ctaagggcgc
      841 ttgggcagga ctctggaatt ggggtgaaag gaggtctctc aaagcaaaag gagtgaaccg
      901 ccacagcgat ggttggaaag gaccctacgg ccttatttgt gattcttgaa cattaggtag
      961 tttaactgga gcggcggacc gggcttgaac cggcaacctc cagcttggaa ggctagagct
     1021 ctaccaattg agctaccgcc gcaaatgctc tactgagcaa aaacgcagaa aacctagtgc
     1081 aaaaagcggc ttctgtcaag aaccagcgca cgtaagttgc atcaaaccga aatcagtccg
     1141 ctgcggaaat cttctcctcg tcctggggag cctggcgttc catctgcagc gatagaatct
     1201 tggagatgcc cttggtctcc atggtcacgc cgtagattgt cgaagccatt tccatggttc
     1261 gcttgttgtg cgtgatgatc aggaactggg aacgctcgct gaactcctgg atcatgcggc
     1321 agaagtttcc gatgttctgg tcatccagcg gggcgtccac ctcgtcaaaa aggcagaacg
     1381 gactgggctt ggtcaggtac atcgcaaaca gaagcgcgat ggccgaaaga gccttttctc
     1441 cgccggaaag aaggttgatg ttctgcagtt gtttgcctgg tggctgcacg acgatttcga
     1501 tgccaccatc gggattgttt tccaggtcaa tgaagaccaa gtctgccttg ccacctcgaa
     1561 acatcttgcg gaacacgtct cggaaattct ctcggatggt gttgagtgcc tcatggaatt
     1621 tttcgctggc agttttctgc aatgtctcga tggttcgaag caacgaagcc cgtgccttct
     1681 ccagatccgt atgctgttcg tgaaggaagt tcagtcgctg ttcgacttcc tcgaactcct
     1741 ggatcgccaa cgggttgact tctcccagtt gtccgatctt gcgtcggact tccgctagag
     1801 actccatcaa gacctctgtc ggccgttcat cattgggtgc ggcctgaatc acctcatcga
     1861 gcggtttttc gaattcctgc tgaatgcgtt cttcgagatt cttgtattcc atttcgaccc
     1921 gaagaagcgc tttttcgaag tcattcttgg tcgtgttgtg gatggcatgc gtgtcgttga
     1981 gtgtccgcag ctgccttgcc acttcgtccc gcgccgtcat gacgttctgc agtgaggctt
     2041 cactctcggt gatttcggtg tgaattgcat cggactgcgt gatcagagaa gtaatc
//
LOCUS       DAOQUC010000730         2095 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_67221_length_2095_cov_4.512745, whole genome shotgun sequence.
ACCESSION   DAOQUC010000730 DAOQUC010000000
VERSION     DAOQUC010000730.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67221_length_2095_cov_4.512745"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            65..175
                     /locus_tag="PKH07_11990"
     CDS             65..175
                     /locus_tag="PKH07_11990"
                     /inference="COORDINATES: protein motif:HMM:NF013555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HNT35706.1"
                     /translation="MPDCPQCGKSMRRRKSAKGDFWGCSALPECKGTRPA"
     gene            281..976
                     /gene="queC"
                     /locus_tag="PKH07_11995"
     CDS             281..976
                     /gene="queC"
                     /locus_tag="PKH07_11995"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HNT35707.1"
                     /translation="MSVKNPSLSVVLVSGGMDSCVTAAIAARDHKTAFLHVNYGQRTE
                     QRELQAFQALADHFQVEHRLVVSFGHLGEIGGSSLTDHSIDVSCADLKNLEIPSSYVP
                     FRNANILAMATSWAEVIGASAIFLGAMEEDSSGYPDCRRSFFDAFEEVIDLGTRPETR
                     IRIMTPLIALSKADVVQKGTELGAPFHLTWSCYVREDVACGQCDSCALRLRGFQKAEV
                     PDPLPYAVRPNYL"
     gene            complement(989..1540)
                     /locus_tag="PKH07_12000"
     CDS             complement(989..1540)
                     /locus_tag="PKH07_12000"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNT35708.1"
                     /translation="MQQPVGLVQDSDLKVQTLVRLAGIAQGNTVLVPGCGSGFLNPAI
                     AEQVGSEGRVISCDVSAQMIRRLQKRRLPRNVFSLVASPRSLPVQDEFCDCIFCVNAF
                     HQFADKAQCLKEFQRVLKKQGHLFIADLDQEDPIGSGRQFSPNALLPSRPAMSAFLKD
                     TGFRLLMLRDNPQGYVLRAVRLP"
BASE COUNT          511 a          530 c          561 g          493 t
ORIGIN      
        1 ggattttatc tgatgggtct cgatccatct taaggtctct gtccgagttg gcgagagaga
       61 cacattgccg gactgtcccc aatgcggcaa atccatgcgc cggcgcaagt ccgcgaaagg
      121 cgatttctgg ggttgttctg cccttccgga gtgcaaagga acaagaccgg cgtaaccgga
      181 gtattgacct gactgaccga actgaccgtg ctgaaggcaa agtcaacagg gtcaatcagg
      241 tcaacacact tagcattgca ctgaaatttg gagatcgaat atgtccgtaa agaatccgtc
      301 cctgtcggtg gttttggtaa gtggtggcat ggacagttgc gtgacggcag ccattgccgc
      361 ccgcgatcac aagaccgcct ttctgcacgt caattacggg cagcgaacag agcaacgtga
      421 attgcaggct tttcaggcat tggcggatca ctttcaggtg gagcatcgcc ttgttgtgag
      481 ttttggacat ctgggcgaga tcggaggctc cagcctgacc gaccactcga tcgacgtatc
      541 ctgtgctgat ctgaaaaacc tggagatccc ctcttcgtat gttccctttc ggaatgcaaa
      601 cattctcgcc atggccacca gttgggcgga ggtgattggc gcctcggcga tcttccttgg
      661 tgcgatggaa gaggacagtt cgggctatcc ggactgccga cgctcattct tcgatgcgtt
      721 tgaagaagtc attgatttgg ggactcgacc tgagaccaga atccgaatca tgactcccct
      781 gattgcgcta agcaaagcgg atgttgtgca gaaaggaaca gaactgggtg caccctttca
      841 tctgacgtgg tcttgttatg tgagagaaga tgtggcttgt ggccaatgcg acagttgtgc
      901 tctcaggttg cggggatttc agaaggcaga ggtacccgat cctcttccct acgctgtcag
      961 gccgaactac ctctgatctc catgccgatc agggcagacg aaccgctcga agcacgtacc
     1021 cttgaggatt gtcacgcaac atcaacagac gaaatccggt atccttcaga aatgcgctca
     1081 tcgcaggtcg tgaaggcaga agcgcattgg gagagaattg gcgtcccgag ccaatgggat
     1141 cttcctgatc gaggtctgcg ataaacagat gcccctgctt cttgagcacg cgctgaaatt
     1201 ccttcagaca ctgggctttg tcggcaaatt gatgaaatgc gttcacgcaa aagatgcagt
     1261 cacagaattc gtcctgaacc ggcagcgagc gaggcgatgc caccaacgaa aacacgtttc
     1321 ggggaaggcg tcgcttctga agacggcgaa tcatctgagc ggagacgtca caactgatga
     1381 ctctcccttc ggaaccaacc tgttccgcaa ttgcaggatt cagaaaacca gaaccgcatc
     1441 ctggaaccag aacggtgttg ccctgagcaa ttccggcaag tcggactaag gtctggactt
     1501 tcagatcaga gtcctgtaca agtccgaccg gttgctgcat tggctcgatc attcctctgg
     1561 aaaaaggtgt cggttcacat atagattctt ggcaccgcat cggcatcgaa actatttcct
     1621 cctaaggaaa ccacttgaaa atctgctctt cagatacttc ggctccttct cctcatccca
     1681 cttgaggtga gagagtcgaa gctgctgacc cgaacagggg tacatttgac ttggatgcga
     1741 cccgcatccg aacttctctt cacatgtcgt acagttaact gaacgttttc acggccgaaa
     1801 agaaacgcca gactctacac cttaatacgc agatttcaaa caaattgttc attttttatt
     1861 cacaatcagc tcgaaaaaga gtaatcttca taagaatggg tgatgtgtac gatgacgttg
     1921 ggatagtgtt gatcttggct tgcagggcgt gttttgtcga aaaggagtct tcttggtgcc
     1981 ttccggtcac agtcagatac ttcagtcgag cggaaaaggc caagtgaacc agccaagggg
     2041 tgttccgaca ccgcagacac cggagcagcc ttttctgagg cgttcagggg acaga
//
LOCUS       DAOQUC010000731         2095 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_67246_length_2095_cov_2.890196, whole genome shotgun sequence.
ACCESSION   DAOQUC010000731 DAOQUC010000000
VERSION     DAOQUC010000731.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67246_length_2095_cov_2.890196"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..870)
                     /locus_tag="PKH07_12005"
     CDS             complement(<1..870)
                     /locus_tag="PKH07_12005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HNT35709.1"
                     /translation="MKKIASRFSICWTLVGAVLVFLLVGVNDIHAAAGDIYTTDYSLA
                     AVLLINPANGSRTILSQSPSYGTGTAFSGPRGLCLSGANLYVVDNTQQAIYRVVISTG
                     NRTVISKSGTAGTGTAFSNPRGIAVDAGGNLLVTDQTLQAVFKVDPSTGNRTVLSKSG
                     TAGTGTAFENPLGIAVHSSGTLYVTDSVANAVFSVDPTTGNRSIISKSGTAGTGTAFT
                     TPRGICVDGSGKILVADSGGTSLLFEVDPSNGNRTVISGGGTGSGTAFGNPYGVRLAD
                     GSNVLVSDNKLSVL"
     gene            complement(950..1489)
                     /locus_tag="PKH07_12010"
     CDS             complement(950..1489)
                     /locus_tag="PKH07_12010"
                     /inference="COORDINATES: protein motif:HMM:NF019126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HNT35710.1"
                     /translation="MDGYLGEIRLFAGTFVPEYWASCDGSLVSVSANMALYSIVSTTF
                     GGDGVNNFALPNLNGKVAVGPGTGFTHGQSGGNQNVTLSTANLPVHTHSIPAHSHALN
                     GSSSAATGTSPSGALPAVTDENAYGTTNLAAMHSSAVGDSSVGTSGSTGSASSFSVMQ
                     PYQVVRYIICVSGYYPSRP"
     gene            complement(1544..>2095)
                     /locus_tag="PKH07_12015"
     CDS             complement(1544..>2095)
                     /locus_tag="PKH07_12015"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNT35711.1"
                     /translation="IRLVERVLDTRLVEKRSTQFQKIQLRPVGRKDESFLRKVFAASR
                     DAEMEHSGWTAEQKKSFVKAQSKLQREDYVRRHPGADFCVIECNKTPVGVFHMDRAPE
                     HLWLMDIAVLKGYRDMGIERRLVERLLTEATRAQKPVRLNIEKSNPYLALFREMGFVQ
                     VEDQGFRIQLEWVPRSRVAVRAL"
BASE COUNT          487 a          626 c          493 g          489 t
ORIGIN      
        1 caagacactc aacttattat ctgaaacaag aacgttactt ccatccgcga gccgaacgcc
       61 atacggattt ccgaacgccg ttcctgaacc ggttccgcca cccgagatga cagttcggtt
      121 cccattggac gggtccactt caaacagcag acttgtgcca ccgctgtcgg caacaagaat
      181 cttcccagaa ccatcaacac agattccacg tggtgttgta aatgcagtac ccgttcctgc
      241 cgttccactc ttggaaataa tgcttctgtt ccccgtcgtt ggatccacgc tgaagacggc
      301 gttcgccacg ctatcggtga catagagggt tcccgaagaa tgaaccgcaa tgccgagcgg
      361 attctcaaat gccgttccgg ttccggccgt accggacttc gacagaactg ttctgttgcc
      421 tgttgaagga tcgactttga agactgcctg caaggtctgg tcggtgacca gcagattgcc
      481 tccagcgtct acagcaatgc ctctcgggtt ggaaaatgcc gtgccggttc ccgctgttcc
      541 gctcttggag atgacagtcc ggttgccggt gctgatgacg acgcgataga tcgcttgctg
      601 agtgttgtcc acaacgtaga gatttgctcc cgaaagacac aaacctcgcg gaccgctgaa
      661 tgcggttcct gttccatacg atggactctg ggagaggatc gttcgactcc cgttggccgg
      721 gttgatcaac aagactgcag caaggctgta atccgttgta tagatgtccc ctgcggcggc
      781 gtgaatatca ttgacgccca ccagcaaaaa caccagcact gcaccaacca acgtccagca
      841 tatactgaac cgactggcaa tcttcttcat aggtctctcc aaatcaaatc gcttatggca
      901 cacccagtag cattcccatt ccatgaaaga aaaacggcag tccgtaattt catggtcgtg
      961 acggataata cccacttaca cagataatgt acctgaccac ctgatagggc tgcatgacgc
     1021 tgaatgaaga ggcactgcca gtgctgcctg aagtcccgac ggaggaatcg cccacggcgc
     1081 ttgagtgcat tgctgccagg ttggtcgttc cgtacgcatt ctcatccgtc acggcgggca
     1141 gggctccaga tgggctggtt cctgttgctg ccgagctgct gccgttcaga gcgtggctgt
     1201 gtgccggaat cgagtgtgta tgaacaggaa gattcgctgt ggacagggtc acattctggt
     1261 tcccgccact ttgcccgtga gtgaaaccag tacctggccc aactgccact ttgccgttaa
     1321 ggttgggcag tgcaaagttg tttacgccgt caccaccgaa ggtcgtactc actatcgaat
     1381 acaacgccat gttggcactc accgacacca gcgaaccgtc gcaggacgcc caatactcag
     1441 ggacaaaggt gcccgcgaat aatcgaatct caccaagata tccatccatc ttaccttact
     1501 cctctctcaa aaacctcatc cctgttccag gaatctcttt cctctacaaa gccctaacgg
     1561 caacacggct cctgggtacc cactccagct gaatccgaaa accctgatcc tcaacttgca
     1621 caaaccccat ctccctgaag agagcaagat agggattgct tttttcgata ttcagtcgga
     1681 caggcttctg tgctctggtt gcctcagtca aaagcctctc cacaagacgc ctctcaattc
     1741 ccatatctcg gtatcccttg agcactgcaa tatccataag ccacaggtgc tctggcgctc
     1801 tgtccatatg gaagactcct accggtgtct tattgcattc gatcacgcag aagtcagcgc
     1861 caggatgcct tcgcacataa tcttcacgct gcagtttcga ctgagccttg acaaaactct
     1921 tcttctgttc tgccgtccat cccgagtgct ccatctctgc gtcccgtgag gcggcaaaaa
     1981 ccttgcgcag aaaactctca tcctttcgac ccaccggccg aagttggatt ttctgaaact
     2041 gggtcgagcg cttctcaaca agcctggtgt ccaagactct ctccactagg cgaat
//
LOCUS       DAOQUC010000732         2095 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_67247_length_2095_cov_2.832843, whole genome shotgun sequence.
ACCESSION   DAOQUC010000732 DAOQUC010000000
VERSION     DAOQUC010000732.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67247_length_2095_cov_2.832843"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="PKH07_12020"
     CDS             <1..319
                     /locus_tag="PKH07_12020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35712.1"
                     /translation="SVDRLNVLSTPVSYLKDPQLVDPFCTENTGDIFYKYSNFKEAPE
                     ALGDPYHLNWGLRGRAPDTVFSRTRDILTNGAINATALYDPTNGITSNGDILRTGSRG
                     LAK"
     gene            complement(380..>2095)
                     /locus_tag="PKH07_12025"
     CDS             complement(380..>2095)
                     /locus_tag="PKH07_12025"
                     /inference="COORDINATES: protein motif:HMM:NF039643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HNT35713.1"
                     /translation="VAEDDRGKARTEQLGREIVALLSCAPNPYKCRLLGTPETVSDRP
                     MRIDGGIGMRHSIWVMIIAAMVFVSKGDCLMSDESIEFWAAHSSTKVGQADARPESAA
                     SSLSIAAARGEYEAAQLVLSASKPLRGVRVCTGHLRRRRIGSISNRNIEVRYACYVPM
                     PVHGRSAPDPLPLLDQIDVEPGKNLPIFVIVRVPRNARAGIYTAPVQIEWEGGKTQTT
                     LTLTVWDFSLPKKPSCVTAFGYIKDLIAQAHGLEMQSARAQELAAKYCAMMLDYGLSP
                     FHIPAPYSSRETRRFLEDPRLTSFVIEYSENDDELAETIRLLKDRGWWSKGYFYIQDE
                     PCNEEQYQRLRQTSQRVKSFAPDARIVSPFFRRPEFDETKSIFDLAADDVNIWCISSH
                     HYDLDPVVREQYHKMKNRGDTLWWYVCCGPGSPYCNFFVEMSGMQHRMLFWQQKMNHV
                     EGLLYWCTTYWNSDPSQGTGNDPWTDMATVKFINPNIYGDGSLFYPGNKVGFDGPVLS
                     QRMLIIRDGIEDFEYLTILQERRGDRKAEEMISRLVTSMTEYCQAPEQLEQIRSEIAH
                     DIEGK"
BASE COUNT          467 a          581 c          533 g          514 t
ORIGIN      
        1 ttcggttgac cgtctgaatg ttctttcgac tccggtgtcc tacctcaagg atccacaact
       61 cgtggatcct ttctgtaccg agaacacagg ggacatcttc tataagtaca gcaattttaa
      121 ggaagcaccg gaagcattgg gtgatccgta ccacttgaat tggggactta gaggacgggc
      181 tccagacacg gtctttagtc gcacgcggga tatcctgacc aacggtgcca tcaacgccac
      241 agccctctac gatccgacca atggcatcac cagcaacggt gacattcttc gaacaggctc
      301 tcgggggctt gccaaatagg ccgttccgat tgcgttcggt tcgcgagttt cttttgcctg
      361 ttccatcctg cttacttgtt cacttgcctt cgatatcgtg cgcgatctca gatcggattt
      421 gctccagttg ttctggagcc tggcagtact ctgtcatgga agtgaccaat ctggagatca
      481 tctcttcggc ctttctatcg ccgcgtcgct cttgcaggat ggtcagatat tcgaaatcct
      541 cgataccgtc tcgaatgatc agcatcctct gggaaagcac tggaccgtcg aaaccgacct
      601 tatttccagg gtagaacaag gaaccatctc catagatgtt tggattgata aacttgaccg
      661 ttgccatgtc cgtccatggg tcgttgcctg ttccctgtga gggatcagag ttccaatagg
      721 ttgtacacca atacagcaga ccctcaacgt ggttcatctt ctgctgccag aacagcattc
      781 gatgctgcat cccggacatc tccacaaaga agttgcagta gggactcccc gggccacagc
      841 acacatacca ccagagagtg tcaccacgat tcttcatttt gtgatattgc tctcgaacga
      901 cgggatccaa gtcatagtga tgcgagctga tacaccagat gttcacgtca tccgctgcca
      961 gatcgaaaat gctcttggtt tcatcgaact ccgggcggcg aaagaaggga gagacaatcc
     1021 gggcgtcggg ggcgaatgac ttcacgcgct gggaagtctg gcgaagtcgc tgatactgct
     1081 cttcattgca cggttcatcc tggatgtaga agtacccctt cgaccaccag cccctgtctt
     1141 tgagaaggcg gatcgtttct gccagttcgt catcgttttc cgagtattcg ataacgaagc
     1201 tcgtgagacg gggatcttca agaaaacgcc ttgtttcacg tgaggagtag ggagccggaa
     1261 tatgaaaggg agagagtcca tagtcgagca tcatcgcaca gtatttcgcc gccaattcct
     1321 gcgccctcgc gctctgcatc tccaaaccgt gcgcttgggc gatgagatct ttgatgtagc
     1381 caaacgctgt cacacaactg ggctttttcg gaagagagaa gtcccagacc gtaagcgtca
     1441 gtgttgtctg tgtcttaccc ccttcccatt cgatctgtac cggagccgtg tagatgccgg
     1501 cccgcgcatt tcgaggaact ctgacgatga cgaatatggg aagattcttc ccgggttcaa
     1561 cgtctatctg atccaacaac ggcagagggt ctggagccga acgcccatgc actggcatgg
     1621 ggacatagca cgcatagcga acctcgatgt ttcgatttga gatggagccg attcgacggc
     1681 gacgcagatg acccgtacac acgcgcacgc ctcgcaaggg tttgctggcg gagagaacaa
     1741 gttgcgccgc ctcgtattca ccacgcgctg cagcgatgga cagggaggat gctgcgctct
     1801 caggtcttgc atctgcctgt ccgacctttg ttgaactgtg cgccgcccag aactcaatgc
     1861 tttcatcact catcaggcaa tctcctttcg acacgaaaac catggcagca ataatcatga
     1921 cccaaatcga gtgtctcatt ccaatgcctc catctatgcg catcgggcgg tctgataccg
     1981 tctccggcgt tcccaagagt ctacacttgt atggatttgg tgcgcaagag agcaaggcta
     2041 caatctctcg tcccagttgc tcggttcttg ctttgcctct atcgtcctct gcgac
//
LOCUS       DAOQUC010000733         2094 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_67289_length_2094_cov_3.094164, whole genome shotgun sequence.
ACCESSION   DAOQUC010000733 DAOQUC010000000
VERSION     DAOQUC010000733.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67289_length_2094_cov_3.094164"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..906)
                     /locus_tag="PKH07_12030"
     CDS             complement(<1..906)
                     /locus_tag="PKH07_12030"
                     /inference="COORDINATES: protein motif:HMM:TIGR03991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sugar nucleotidyl transferase"
                     /protein_id="HNT35714.1"
                     /translation="MGRCLMVFEDAGYSNLQPLTYTRPVYDLKCGATSLLDKILRAYG
                     VKACCVHTRNYLADLVAEQKAGVNVNKTCDDDVLLVNGRVLADAELARKIPMDGPETV
                     FTTADGTMVAARISKAKAKELFASKPDTLCPKCVGAGLPTQTVEATLINYPWDLFHHN
                     GKAIAADFQAMAKPGVLEGKVYEGVHILAKENVYVAPGATVKPGVVLDAEGGPIFIDA
                     DAKIFPNAVIEGPTYIGKKTAIKIGAKIYEGTSIGEVCKVGGEVEESIIHSFSNKQHE
                     GFLGHAYLGMWVNLGADTNNSDLKND"
     gene            complement(942..1715)
                     /gene="nagB"
                     /locus_tag="PKH07_12035"
     CDS             complement(942..1715)
                     /gene="nagB"
                     /locus_tag="PKH07_12035"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013042291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006046 - N-acetylglucosamine catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HNT35715.1"
                     /translation="MEVIIKADYAEMSKEAAKIIANLLYEKPNAVLGLATGSTPIGLY
                     KELVRMHKEEGLDFSKVVTFNLDEYIGLPATHDQSYNYFMNDNLFKYINVNPENVHVP
                     NGMARDVEKFCEWYEDEIDKAGGIDLQVLGIGGDGHIAFNEPGSSLASRTRMKTLTEE
                     TIVDNARFFANKDDVPRYAITMGVGTILEAGHCLMLANGEKKAGTVKKAIEGPVTQMV
                     TASALQLHPQVTVILDEPAASKLELKDYYKWVYNNKPKC"
BASE COUNT          439 a          560 c          552 g          543 t
ORIGIN      
        1 gtcattcttc agatcgctgt tgttggtgtc agcaccgagg ttgacccaca taccgaggta
       61 ggcgtggcca aggaagccct catgctgttt gttggagaaa ctgtgaataa tggactcttc
      121 gacttcacca ccgactttgc agacttcgcc gatgctggtg ccttcgtaga tcttggcgcc
      181 aatcttgatg gcggtcttct tgccgatgta agttgggcct tcgatgaccg cattcgggaa
      241 gatctttgcg tcggcatcaa tgaagatcgg gccgccttca gcgtccagga cgacgccagg
      301 cttcacggtc gctccgggag cgacgtagac attttccttg gcgagaatgt gaacgccctc
      361 atagaccttg ccttcaagaa cgccaggctt tgccatggcc tgaaaatcag ccgcgatggc
      421 tttgccgttg tggtggaaca aatcccacgg atagttgatc agggttgctt cgaccgtctg
      481 cgttggcagg ccggcaccga cgcacttggg gcagagggtg tccggcttgg aagcaaacag
      541 ttcttttgcc tttgccttgc tgatgcgggc ggcgaccatg gttccgtcgg cagttgtaaa
      601 gactgtctcc ggaccgtcca tggggatctt cctggccagt tcagcgtccg ccagaacgcg
      661 accgttgacc agcagtacat cgtcatcaca ggtcttgttg acattgacgc cggctttctg
      721 ctcggcaaca aggtcagcca ggtagttgcg cgtgtgaacg cagcatgcct tcacgccgta
      781 agcccgaagg attttgtcca gcaggcttgt tgcgccacac ttgagatcgt agacaggcct
      841 cgtatacgtg aggggctgca ggttgctgta acctgcgtcc tcgaaaacca tcaaacaacg
      901 tcccattttt tcctccattt tccgagtgcc catgagggga cttagcactt gggtttattg
      961 ttgtagaccc acttgtaata atctttcagt tccagtttgg aagcagcagg ctcgtcaaga
     1021 atgaccgtca cctgcgggtg caactgaagc gcggacgcgg taaccatctg agtcacagga
     1081 ccttcgatcg ccttctttac ggttccggct ttcttctcac cgttggcgag catcaggcaa
     1141 tgacctgctt cgagaatcgt tccgacgccc atggtgatag cgtagcgggg aacatcgtct
     1201 ttgttggcaa agaaccgcgc attgtccacg atggtttctt cggtcagagt cttcatgcgc
     1261 gttctggacg caagcgaaga acccggctcg ttgaacgcaa tatggccgtc tccaccaatg
     1321 ccgagaacct gcaaatcaat gccaccggcc ttgtcaatct cgtcttcgta ccattcgcag
     1381 aacttctcga cgtcgcgagc catgccattg ggaacatgga cgttctccgg attgacgttg
     1441 atgtacttga acaggttgtc gttcatgaag tagttgtaac tctgatcgtg ggtggcgggc
     1501 agaccgatgt actcatccag gttgaaggta acgaccttgg agaaatcgag gccctcttcc
     1561 ttgtgcatcc gaaccagttc cttgtacaat ccgatgggtg tacttcccgt ggccagaccg
     1621 agaaccgcgt tgggcttctc gtagaggaga ttggcaataa tcttggccgc ctctttcgac
     1681 atctctgcat aatccgcctt aataatgact tccatcctac atgcgctcct tctgctgatt
     1741 attggttccc ttaggattgt ccgtgggtga acctattaag aaagaacact cccgatgagt
     1801 gtgttttctt ctcaatcaca ggtgaccgaa gaggcgggca caacaataca gcccgcagtc
     1861 tgccagaagt acggttcaac actcgataat ggttttcctg caccgtacta caggaacgtt
     1921 tcttatcacc ccgggcgtaa cgtttgtcaa gaacaatttt tgcttttttc ctgtaatttc
     1981 aatgatttgg gagactgttg gctgttaatc caatcacaga gaaccgcgat gtctgtcgtt
     2041 tcccgtccaa gggtgagctg gagcattgcc ccacaagcga tcagatttct tctc
//
LOCUS       DAOQUC010000734         2090 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_67522_length_2090_cov_3.042752, whole genome shotgun sequence.
ACCESSION   DAOQUC010000734 DAOQUC010000000
VERSION     DAOQUC010000734.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67522_length_2090_cov_3.042752"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(309..1283)
                     /locus_tag="PKH07_12040"
     CDS             complement(309..1283)
                     /locus_tag="PKH07_12040"
                     /inference="COORDINATES: protein motif:HMM:NF025875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HNT35716.1"
                     /translation="MTERQSHQTTQDSLSLGRFRITAVVLIVMCLLAFACREDEYGVR
                     FSHRNHVTERGLDCAVCHGELLEGSFSKPNHDSCVGCHEEFIESATLSLQTCGFCHAV
                     REVDTLSEPMPSPVRPVRGVFRHSEDLDGRCEECHGEMLDRDVEIMHVMKRDQVIQWR
                     NRAHASATSCNGCHDGQSKNKQPDTHQRDWVRRHGARYTEDTSACQVCHTEQSCQECH
                     QANKPTNHNNLWRMKAHTVQASWRRDYCRTCHQEDFCAGCHQEVKPRSHKVAWRRRHC
                     YNCHTSPSQTTGCVVCHPGKGVERHQDKTPPFHLDTVPGLNCMICHRP"
     gene            complement(1293..>2090)
                     /locus_tag="PKH07_12045"
     CDS             complement(1293..>2090)
                     /locus_tag="PKH07_12045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35717.1"
                     /translation="SLTRLTYVHYSDDRSDDDWYLLEIWQRLTDEFRLHARTSMLDNN
                     FQMGSIDLYYLSLNGELDVIVTASRWSGISQQSRQYSPIYQVLGDQQAFTFLSARATQ
                     SVLPWLSLSCGAAARLADDSDRQVRDRQYGTGDVTLIVQPTNQWTFSLAGEYWDVSAG
                     DHFWGITGEVEYRPDDRWEAALGTGYLDYQYHEYSDLRYYYAFGGLQLDYDGTRTMIS
                     PDVYTCYGRLKHRLNQWTAISMRGEIENNSLEDNTAYCLRTSLDIRF"
BASE COUNT          468 a          559 c          573 g          490 t
ORIGIN      
        1 ggtcttgcat ttcttgactc cagtgtactc aggagaaggg gaatccgctc ccacaacgaa
       61 gacgatggca agacaaaaga gagatacggt tgcagcaaaa cgaagataca tggcgaatct
      121 ccttttccca aatgacactc aatgcagagg aagcgtgcca tttgctaagc aagcatccaa
      181 gggagtgggc atagcccaca cgcccgcagg tgccaacctg ctcaggaatt cctggctaga
      241 aggatagcac aaatcccgat aggcgccctt tgcgagatgg aaacaagtca ctgtgctcca
      301 aaacccctct agggtctgtg gcagatcatg caatttagac cggggacagt gtccagatga
      361 aacggcgggg tcttgtcctg atgacgttcg accccttttc ctgggtgaca gacaacacat
      421 cctgttgtct gtgacggtga cgtgtgacag ttgtagcagt gtctgcgccg ccacgcgact
      481 ttgtgggatc ggggttttac ttcctggtga caaccggcgc agaagtcttc ctgatgacag
      541 gttctgcaat aatccctgcg ccacgatgcc tgaacggtat gggctttcat ccgccacagg
      601 ttgttgtgat ttgtgggctt gtttgcctgg tggcactcct ggcagctctg ctcggtgtgg
      661 cagacctggc aggcgcttgt gtcttcggtg tatcgagcac cgtggcggcg aacccagtct
      721 ctctggtggg tatccggctg tttgttcttg ctttggccgt cgtggcagcc attgcaggac
      781 gttgccgagg cgtgagcccg attccgccat tgaatcacct gatccctttt catgacgtgc
      841 atgatttcga cgtccctatc aagcatctct ccgtgacatt cctcgcaccg tccatccagg
      901 tcttccgaat gtcgaaagac accgcgcaca ggtctgacag gcgagggcat cggctcggag
      961 agagtatcca cttctctcac cgcgtgacag aagccacacg tttgtagaga gagcgtcgcc
     1021 gattcgatga actcttcgtg acagccaaca caggagtcgt ggttgggttt gctgaatgaa
     1081 ccttccagaa gttcaccatg acagacagcg cagtccagcc ctctttctgt gacatggttt
     1141 cgatgactaa agcgaacgcc atattcatcc tcacgacaag cgaaggcaag cagacacatg
     1201 acaataagca cgaccgccgt gatccgaaac cgtcccaggc tcagggagtc ttgtgttgtc
     1261 tggtgactct gtcgctcggt cattgcactc ctctaaaagc gaatatccaa agatgttcgc
     1321 aggcagtaag cggtgttgtc ttccagagag ttgttttcga tttcgccccg catactgatt
     1381 gcagtccatt ggttcagacg atgtttcagt cggccgtagc aggtgtacac gtcgggagag
     1441 atcatggtgc gtgttccatc gtagtccagc tgcagtccgc cgaaggcata gtagtaccgt
     1501 aaatccgaat actcatggta ctgatagtcg agataaccgg tacccagggc tgcttcccaa
     1561 cgatcatcgg gacgatattc cacctcgcct gtgattcccc agaaatggtc gcctgcgctg
     1621 acgtcccaat actcaccggc aagtgagaat gtccactggt ttgtgggttg gacgatgagc
     1681 gtgacatcac cggtgccgta ctgccggtct cgcacctgtc ggtctgagtc atcggcaagt
     1741 ctcgctgctg ctccgcaaga cagactgagc caaggcagca cagactgtgt cgctcgtgcc
     1801 gagagaaacg tgaacgcctg ctgatctccg aggacttggt agatcggtga atactgtcgt
     1861 gactgttgtg agatccctga ccagcgggac gctgtcacga ttacatccag ttctccatta
     1921 agtgaaaggt aatacagatc aatggaaccc atctgaaagt tgttgtccag catggacgtt
     1981 cgcgcatgaa gcctgaactc atcggtcagt ctctgccaga tttccagaag ataccagtcg
     2041 tcgtcagaac gatcgtccga gtaatggacg taggtcaggc gggtcaggct
//
LOCUS       DAOQUC010000735         2090 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_67526_length_2090_cov_2.851106, whole genome shotgun sequence.
ACCESSION   DAOQUC010000735 DAOQUC010000000
VERSION     DAOQUC010000735.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67526_length_2090_cov_2.851106"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..421)
                     /locus_tag="PKH07_12050"
     CDS             complement(<1..421)
                     /locus_tag="PKH07_12050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35718.1"
                     /translation="MAMTLRERMLAFVQGREMDRVPFCEYSTICGDSNETVWELVGRE
                     NMGLLAGVHAHRIEHPNCQFENEEYEFQGNRAFRATLHTPAGDLVQEAVQEPTFGTCT
                     RRKSFVREPKDYEVLRSYLKDITIYPYTEGLENVLKGL"
     gene            complement(461..1669)
                     /locus_tag="PKH07_12055"
     CDS             complement(461..1669)
                     /locus_tag="PKH07_12055"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNT35719.1"
                     /translation="MNVAEIPGYEVLNQDHIKIARELVEEARAHDGMIPMDLEKFWAD
                     NHLANSNPFGAEIPQVAFGATLTSECVFDELGVPEDVWRYHNDADWRLELNKAYNVKS
                     EAIVGRRLVSENPPGDPTKHYPGVKGLHDVFEARNEWHNGSWWLMQSAHDEDELKALL
                     DRVEQCDIRSFILPPNWEEEKKRLMAMDIRPHLYRGQRGPVTFAASVYGAENLLFLIL
                     DNPELAVRFRDAILKTMLEIARVRDEEAGYTPETAPHGFSFCDDNCCLLNPEMYELFG
                     YPIIKGIFERYCPDPGDSRFQHSDSAMGHLLPLMARLGMNRVNFGPTVMVNEIRKHLP
                     NAIIYGQLAPFTYSRNEEERMVLEFFRDFEMSRQSRGLVFTTAGSINNGTRLTSMRLL
                     MATIQRYGRF"
     gene            1907..>2090
                     /locus_tag="PKH07_12060"
     CDS             1907..>2090
                     /locus_tag="PKH07_12060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35720.1"
                     /translation="MPKKTSVTGSELFIVDNSDQDWKALKYLHDWCQISEQIDIATGY
                     FEIGSLLALGDEWHKVD"
BASE COUNT          456 a          535 c          554 g          545 t
ORIGIN      
        1 caagaccttt cagcacattc tcaagacctt ccgtgtaagg atatatcgtg atgtccttca
       61 gataagagcg cagcacttcg tagtccttcg gttcacggac gaaacttttt cgtcgtgtgc
      121 atgtgccgaa ggttggctcc tgaactgctt cctgaaccaa gtctcctgcc ggtgtgtgaa
      181 gcgttgcgcg gaatgcgcga ttgccctgga attcatattc ctcgttctcg aattggcagt
      241 tgggatgttc gatgcggtga gcgtgaacgc cggcgagaag tcccatgttc tctctgccaa
      301 cgagttccca gactgtttcg ttggagtctc cgcagatcgt gctgtactcg cagaagggaa
      361 cccgatccat ttcgcgtccc tggacgaatg ccagcattct ttcacgcagg gtcattgcca
      421 tctatgtgtt ctccttcgaa aagcggtctt cagagccggt tcagaatctg ccgtagcgct
      481 gaatggttgc catcagcagt ctcatggatg tcaaacgcgt tccattgttg atggagccag
      541 cggttgtaaa aaccagtcct cttgattgtc tggacatttc gaagtcgcgg aagaattcga
      601 gtaccatccg ttcttcttca tttcggctat aggtaaaagg cgcaagctgt ccatagatga
      661 tagcgttggg aagatgcttg cgtatttcgt tgaccatcac cgtcggtccg aaattgactc
      721 gattcatccc caacctggcc atcagtggca gcagatgacc catggctgaa tccgaatgct
      781 gaaaccttga gtcgcccgga tcggggcaat agcgttcaaa gatgcctttg atgatgggat
      841 agccaaagag ttcatacatt tcgggattca gcaggcagca gttgtcatca cagaaagaga
      901 atccatgtgg tgcggtctct ggtgtgtagc ctgcttcttc atcgcgaaca cgggcgattt
      961 ccagcattgt cttcaggatg gcatctcgga aacgtacagc aagttctgga ttgtccagga
     1021 taaggaaaag cagattctca gcgccgtaga ccgatgcggc gaacgtgacc ggcccgcgct
     1081 gacctcggta cagatgaggt ctgatgtcca ttgccatcag acgtttcttc tcctcttccc
     1141 aattgggggg aagaatgaaa ctgcgaatat cgcattgctc gacgcgatcc aagagtgcct
     1201 ttagttcatc ctcatcgtgg gcactttgca tgagccacca ggagccgttg tgccattcgt
     1261 tgcgagcctc gaagacgtca tgcagacctt tgactccggg atagtgtttg gtaggatcgc
     1321 cgggaggatt ctctgagaca aggcgtctgc ccacgatcgc ttcagatttg acgttgtagg
     1381 ccttgttgag ttcaaggcgc cagtcagcgt cgttgtggta tcgccagaca tcttctggca
     1441 ctcccagttc atcaaaaacg cattctgaag taagcgtggc tccaaaagcc acttgaggaa
     1501 tttccgcccc gaagggattg ctgttggcga ggtggttgtc cgcccagaac ttctccaagt
     1561 ccatgggtat catcccgtcg tgagcacgag cttcctccac cagttctctg gcgatcttga
     1621 tatgatcctg attcagtact tcgtaaccgg gtatctcggc aacgttcatt gaactccctt
     1681 tctctccgat ggtgtggact ctgctccagt cccagaaatg tttcgattgt gtaccctatt
     1741 ctgcatcgct tcgtcaagtg ggcagaaggg agtcagaaca tcttgccccc ggtctgcctc
     1801 cagagctcct ttcatttccc tcgagcgaaa ggcattctgg cttaccggag acttttttgc
     1861 tagtcttgcg gcgaatcgtt caacatagtc attgggagag acgaccatgc caaagaagac
     1921 aagtgtcacg ggcagtgagc tgttcattgt ggacaacagc gatcaggact ggaaggcact
     1981 caagtacctg catgactggt gtcagatctc cgagcagata gacattgcga ctggctattt
     2041 cgagatcggc tctctgcttg ctttgggtga cgaatggcac aaggtggaca
//
LOCUS       DAOQUC010000736         2089 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_67563_length_2089_cov_3.262045, whole genome shotgun sequence.
ACCESSION   DAOQUC010000736 DAOQUC010000000
VERSION     DAOQUC010000736.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67563_length_2089_cov_3.262045"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            178..1017
                     /locus_tag="PKH07_12065"
     CDS             178..1017
                     /locus_tag="PKH07_12065"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNT35721.1"
                     /translation="MSHRDPFTITDSAGVKIPCRQWQASDPKSAIVFVHGIQSHSGWF
                     DEACCFLSERGHTCYGFDRRGAGISEQPRGYIDSYRTWIEELTTVLQFAESESEGRPV
                     HLLSWCFGAKVALGYLLSTRENSLASAIFLCPGIKTKTQLRWRQMLRVLVGSKSPDAT
                     PMETPISDEMFTDVPEYLEFIHNDPHSLRKATPALYFQIFLLDRFLRKSSNKITLPLL
                     FMLAGQDQIADNEYAVKFYAEACSQVKRLISYANVRHSLFFSTERQRVMDDLCLWLDH
                     KEQ"
     gene            1017..>2089
                     /locus_tag="PKH07_12070"
     CDS             1017..>2089
                     /locus_tag="PKH07_12070"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HNT35722.1"
                     /translation="MRIKSFDVFRLQIPFRLSFSHIKAARRTSDSVLLRCTLADGTVG
                     YGESLPRPYVTGETMDSVADNLANRFLPTLKSFDPSAFEEVSSRCENLYEEMHSAFPE
                     AGKYIGASVCAVELALLDAFGKHFDEPCIPTGSASPDVRFSSVIGSCSKPVLTAFALA
                     TRLWGFQSVKLKIGTDRDDENLRLAHRLLGKRVQLRVDVNGVWSADQALRWLEHNMLN
                     NLVAMEQPVAADDFDGLKRLTKAAPIDIMADESLRDLEDARMLSDQGCCRAFNIRISK
                     CGGLYHSLKIARLANDRGLSVQIGCQVGETSILGAAQLRLVHLLGRVRFLEGCYGSLL
                     LKQDLTKTSLRFGRSGALPDIPKG"
BASE COUNT          457 a          512 c          593 g          527 t
ORIGIN      
        1 gaaaatggtt ctgggtcagt acttgggagc tgcctgtctt atccttgctg gtttgtcctg
       61 ttcctttgga gtgatgcgaa tcgtccatcg aatggactgg tctgatgata gaatggaaac
      121 gtgagaatcg gtgttcgccc tttctctgtg agatacaggt ctgtgagaga tgatcctatg
      181 agccaccgcg atccgttcac tatcaccgat tcagcgggtg tgaagattcc ttgccgtcag
      241 tggcaggcat ccgatcccaa gagtgccatc gtttttgtcc acggtattca gagccattcc
      301 ggatggtttg atgaggcctg ttgctttctg agcgagagag ggcatacctg ctatggattc
      361 gatcggcgcg gcgctggaat cagcgagcaa ccacgcggct atattgactc ctacagaacg
      421 tggattgaag aactcacgac ggtgctgcag tttgcagagt ctgagagcga ggggcgtcct
      481 gttcatttgc tctcatggtg tttcggcgcc aaagtggctc ttggctattt gttgtccacg
      541 cgggagaatt cgcttgcttc tgcaatcttt ctctgccctg ggatcaagac caaaacacag
      601 ttgcgctgga gacaaatgct gcgcgtgctc gtgggaagca agagtcctga tgcgacgccg
      661 atggagaccc cgatcagcga tgagatgttc acggatgtac cagagtacct ggaattcatc
      721 cacaatgatc cccacagcct gcggaaggcc acacccgcac tgtacttcca gatattcctc
      781 ttggatcgct ttttgagaaa gtcttcgaac aagatcacat tgccacttct cttcatgttg
      841 gcgggtcagg atcagatagc agacaatgag tatgccgtga aattctatgc ggaggcatgc
      901 tctcaagtga agcgtctaat cagctatgcc aacgtccgac acagtctctt cttcagcacc
      961 gagaggcaaa gagtcatgga cgatctctgt ctctggcttg atcacaagga acaataatgc
     1021 gaatcaaatc gtttgacgtc tttcgtttgc agatcccctt tcggttgtca ttttcgcaca
     1081 tcaaagcagc acgacgcacc tcggatagcg ttttgctgcg atgcacactt gctgatggaa
     1141 ccgtcggcta cggggaatcg ctgcctcgac cctacgtcac cggagagacc atggacagcg
     1201 ttgcagacaa cctggccaat cgctttctcc ccaccctgaa gtcatttgat cccagcgctt
     1261 tcgaggaagt cagttcacga tgtgagaacc tctatgagga gatgcacagc gcctttcctg
     1321 aagcggggaa gtacattggc gcgtcggtgt gtgctgtcga gttggcactc ctggatgcat
     1381 ttggcaagca cttcgacgag ccttgtattc caacgggcag tgcgtcgccg gatgttcgtt
     1441 tctcctcggt gatcggatcg tgctccaaac cagtcctgac cgctttcgca ctggcaacac
     1501 gcctctgggg atttcagtcg gtgaagttga agatcggtac agatcgggat gatgagaacc
     1561 tgcggctggc acatcgtctg ttgggcaaac gagtccaatt gcgtgttgat gtcaacggag
     1621 tgtggtctgc ggatcaagca ctgcggtggc ttgagcacaa tatgttgaac aaccttgtgg
     1681 caatggaaca acctgttgcg gcagatgatt ttgatggtct aaaacgcttg acgaaggccg
     1741 ctccgataga cattatggca gatgagtcac tcagagactt ggaggacgcc aggatgctga
     1801 gtgaccaagg ttgctgcagg gcatttaaca ttcgaatctc gaagtgcggc ggcctgtacc
     1861 actcattgaa aattgctcgg cttgcgaacg acagggggct gtccgttcag atcggttgcc
     1921 aggttggaga aacgtccatc ctgggtgcgg cacagttgag actggtacat ttgttgggaa
     1981 gggttcggtt tctggagggc tgctatggct ctctgctgtt gaaacaagat ctgacgaaga
     2041 cgtcgcttcg atttggtcgc tcgggggcgt tgcctgatat cccgaaagg
//
LOCUS       DAOQUC010000737         2089 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_67581_length_2089_cov_2.672566, whole genome shotgun sequence.
ACCESSION   DAOQUC010000737 DAOQUC010000000
VERSION     DAOQUC010000737.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67581_length_2089_cov_2.672566"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /locus_tag="PKH07_12075"
     CDS             <1..387
                     /locus_tag="PKH07_12075"
                     /inference="COORDINATES: protein motif:HMM:NF024581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNT35723.1"
                     /translation="SGMNTEEQVVENVEITGRKVPLTDKQRSRFLESLGKRHEKVGVL
                     CNACGYCMPCPFGVDIPRNFTLLNHAKLWGLMDLSRRRFRRLQKSEEGNRSASACKQC
                     GACLPKCPNRIPIIKRLAQAAELFGG"
     gene            430..1374
                     /locus_tag="PKH07_12080"
     CDS             430..1374
                     /locus_tag="PKH07_12080"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HNT35724.1"
                     /translation="MKTIIHSRAWIPGLILLCLSVSSWAAEPTGGEALAVFSSIPPQG
                     YVLERIGGQRLSVTVLLPQGRDPHTFEPTPKQLTEVSKAAVFFTIGLPFEKVIVEKLS
                     NLSDRLRFVDMAEGINRIQISDDEHEHKHEHGESCDHSPDGTDPHIWLSPVLLEVLAT
                     NTARALEQMDAVHSDEYKMNLAHFLAEIRATHLRIQGILEPYRGRTFFVFHPAFGYFG
                     QAYGLKQQAVEIRGREPAPKQLALLIKKAKAENVRIILVQPQFDGRAAQTIASAIGGV
                     VAPMDPLEKNLLQSFENMAALLEKAFSADTKQRTPSEQ"
     gene            1371..>2089
                     /locus_tag="PKH07_12085"
     CDS             1371..>2089
                     /locus_tag="PKH07_12085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNT35725.1"
                     /translation="MTSRDALILKNVTFSYDGLTVLEDVNLTVSEGELLCIVGPNGGG
                     KTTLLKLMLGILVPQRGSIQILGTTPERARPRVSYMAQYVHYDPQFPVTVLDIVLMGR
                     LGTGISGFYSKADKRKALEALDEVGLSGADRRLFSLLSGGQKQRVFIARALASQPSLL
                     LLDEPTASVDVAVEAQFFEVLQRLSKTMTILIVSHDLGFVSSIVQSVVCVNRKVVVHP
                     TSELNGTLIRDLYRDRGSEFHS"
BASE COUNT          473 a          514 c          591 g          511 t
ORIGIN      
        1 tcaggcatga atacggaaga gcaggttgtt gagaatgttg agatcacagg taggaaagtt
       61 cccctgaccg acaagcaacg atcccgtttt ctagagtccc ttgggaaacg tcatgaaaag
      121 gtgggcgttc tctgcaatgc ttgcgggtac tgtatgccgt gtcctttcgg tgtggacatc
      181 ccgcgcaatt tcaccttact caaccacgca aaactctggg gccttatgga tctgtccagg
      241 cgacgattca ggcggctgca gaagagcgag gaaggaaacc gttctgcctc tgcctgcaag
      301 caatgcggtg cctgtctgcc caagtgtccg aacagaattc caatcatcaa acgtcttgca
      361 caggcagccg aattgttcgg tggctagcgg tttctgtttc ggatggacaa cggatttggg
      421 aggtgtgcca tgaagacgat catccacagt cgtgcttgga tacccgggct tatcctgctt
      481 tgtctgtctg tctcctcttg ggctgctgaa ccgactggtg gagaggctct ggctgtcttt
      541 tcgagcattc cgccacaggg gtatgtgctt gaaaggatcg gcggtcagcg gctgtccgtg
      601 accgtcctgt tgccacaggg aagggatcct cacaccttcg agcccacgcc caaacagctg
      661 acggaagtct cgaaagcggc tgtattcttc accattgggc ttccattcga gaaggtcatc
      721 gtcgaaaagc tctctaactt gtctgacagg cttcgatttg tggacatggc agaaggcatc
      781 aacagaattc agatctcgga tgacgagcat gagcacaagc atgagcatgg agagtcatgc
      841 gaccatagtc ctgacggcac agatccacat atctggctct cgccagtgtt gcttgaagtg
      901 ctggccacca acaccgctcg ggctttggag cagatggacg cagttcactc agatgagtac
      961 aagatgaatc tggctcattt tctcgcagag atcagagcca cacatctgcg aatccagggg
     1021 attctggaac cgtaccgagg cagaaccttt ttcgtttttc atccggcgtt cggctatttc
     1081 gggcaggcct atggactgaa gcagcaggca gttgagatac ggggtcgcga gccagcccca
     1141 aagcagttgg cgctgcttat caagaaagca aaagccgaga atgtaaggat cattctggtc
     1201 cagccacagt ttgacggtcg cgcggcgcag accattgcca gcgcgattgg tggtgtcgtt
     1261 gctccgatgg atcctttgga gaagaacctt cttcagagtt ttgagaatat ggctgcactt
     1321 cttgaaaagg ccttcagcgc agacaccaaa caaaggactc catctgaaca atgacctcca
     1381 gagatgcttt gatcctcaag aatgtgacgt tctcctatga cggtctcacc gtcttggagg
     1441 atgtcaatct gactgtttct gaaggggagt tgctttgtat tgtcggtccc aacggaggag
     1501 ggaagaccac tcttctgaaa ctcatgcttg ggattctcgt accccagcga ggcagcattc
     1561 aaatcctcgg tacgacgccg gagagagcac gcccgcgcgt gagttatatg gcgcagtacg
     1621 ttcattatga tcctcagttt ccggtgaccg ttctggacat tgtgctgatg ggcaggctgg
     1681 gaactggaat ctccggtttt tacagcaagg cagataagcg aaaagctctg gaagcattgg
     1741 atgaagtcgg gctttccggg gcagatcgtc gattgttttc gttgctctcg ggtggacaga
     1801 aacagcgtgt cttcatcgca cgggcgttgg cgagtcagcc gtcgctactg ctgttggatg
     1861 aacctaccgc gagtgtggat gttgcggttg aagcacagtt tttcgaggtt ttgcagagac
     1921 tgtccaagac catgacgatc ttaatcgtga gccacgatct gggctttgtg tcctcaattg
     1981 ttcagagtgt cgtatgcgtc aacaggaagg tcgttgtgca tccgacgagt gaactgaatg
     2041 gaactctgat ccgcgatctc tatagagatc gcggatcaga gttccattc
//
LOCUS       DAOQUC010000738         2088 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_67618_length_2088_cov_3.490408, whole genome shotgun sequence.
ACCESSION   DAOQUC010000738 DAOQUC010000000
VERSION     DAOQUC010000738.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67618_length_2088_cov_3.490408"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1108)
                     /locus_tag="PKH07_12090"
     CDS             complement(<1..1108)
                     /locus_tag="PKH07_12090"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HNT35726.1"
                     /translation="MLSLLTFTGMRLPYAAQIVVTDDKAYPPFAFLDSKGTPRGITID
                     IWKLWSERTGVAVEFYLMEWNLALDSVREGRADVVAGLFRTPEREADFDFTKPIIKIP
                     TGLFFNRQIPGIRGVEDLDGFQVGVVRGDSAEELLGLIEPSIETVAYPGTDELVKAAL
                     SDEIKVLIADAPTVLFYLAQHPRGDLFRQTSEPFTTNLQYAAVRKGNRALLEIIQSGF
                     DQISESDINHIVNEWEGRSTLRTLPWQGIGTAFAGIIFVALVVLVWNLLLRRRVREAT
                     DHLAEQNAQLENSRDEIRRSEEQYRLLVESANSIILRMDPLGNVTFFNDFAERFFGFT
                     REEILGRNVVGTIVPKHDDAGRSLQDLILDIGHNP"
     gene            complement(1304..>2088)
                     /locus_tag="PKH07_12095"
     CDS             complement(1304..>2088)
                     /locus_tag="PKH07_12095"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HNT35727.1"
                     /translation="TPAEMVPGFVISDQFFEEFSTDRRAGTVRDVTEYPSTLYWDLPD
                     ADLGIKLDAAWQALEDYPGPYKENLEGMDNVDRDRFYAVLAPAVLTSMRPKLTLVTFY
                     GTQSLGAHFWPYWEPQYFPDVFRQEVSRYHDILPGYYQYLDKVLSELITAVGPQTTVF
                     VVSGYGMEPAIGAPDGRRACHGNMTPGILVASGDSIRQGFGWVERAELVDLASTILYL
                     LGFPISNELDGRVLTEIVKEDFLLRHPIRQIPSYGPRLCITP"
BASE COUNT          527 a          560 c          540 g          461 t
ORIGIN      
        1 cgggattgtg tccgatatcc aggatcagat cctgcaaact tctgccggca tcatcgtgct
       61 ttggaacgat ggttccaacg acgtttctgc ccagaatctc ttctctggta aatccgaaaa
      121 atcgttcggc gaaatcgtta aagaaggtca cgttgcccaa tggatccatc cgcaaaatga
      181 tgctgttggc gctctcaacc agcagtcggt actgctcttc acttcgccga atctcatctc
      241 gggagttctc aagttgagcg ttctgttcgg cgagatggtc ggttgcctct cggacacgac
      301 gacgaagcag caggttccat acaagaacga ccaaggcaac gaagatgatg ccagcaaatg
      361 ccgtcccgat cccttgccat gggagtgtcc gaagtgttga acgtccttcc cactcgttga
      421 cgatatggtt gatgtcggat tccgaaatct gatcgaatcc cgactgtatg atctccaaca
      481 aagcgcggtt tccttttcga accgcggcgt actgcagatt tgtagtgaaa ggctctgagg
      541 tctgccggaa aagatcacct cgtggatgtt gtgccagata gaaaagaacc gttggtgcat
      601 ccgcaataag aaccttgatc tcatcggaca gcgcagcctt cacgagttca tctgtcccgg
      661 gataggcaac ggtctcgata ctcggttcga tgaggccaag cagctcttcc gcactgtcgc
      721 cgcgaaccac ccccacctga aaaccatcaa ggtcttccac ccctcggata cccgggatct
      781 gacggttgaa gaataggcct gtgggtatct tgatgatcgg cttggtgaag tcaaaatcgg
      841 cttcacgttc tggggtacga aacagtccgg ccacaacatc tgctcgccct tcacgaaccg
      901 aatccagcgc cagattccat tccatgaggt agaactcaac ggcaacaccc gtcctctcac
      961 tccacagttt ccagatatca atggtgatgc cccgaggcgt tccttttgaa tcgagaaagg
     1021 caaaaggggg ataggccttg tcgtcggtca caacgatctg agcggcataa ggcagacgca
     1081 tccctgtaaa ggtcagaaga gataacaaga tcaggaggaa tctccgcaat cgattcgctg
     1141 agatgtaaga gaaaggaggc tgcacattat tagccatcgt gctgcgtgtt ctccagagta
     1201 cctagttagc caagacacac cagactcact caatgacaac acactatctt gcacgctatg
     1261 gaggatcgtg tcaaggcagg ttcatgagtt tgacaaaatg cgtctagggc gtgatgcaca
     1321 gcctcggtcc gtaggaaggg atctgacgga tcggatggcg aaggaggaag tcttccttga
     1381 cgatctcggt gagcactctg ccatcgagtt cgttcgaaat cggaaagcca agcagataga
     1441 gaattgtgga agccaggtcc acgagttccg cacgctcaac ccagccaaag ccttgccgaa
     1501 tcgagtcacc ggacgcgaca aggatgcccg gagtcatgtt gccatgacaa gccctgcgtc
     1561 cgtcgggagc ccctatggct ggctccatgc cgtagcctga aacaacaaat acggttgtct
     1621 gaggaccaac tgccgtgatc agttccgaga gcaccttatc cagatactga taatagccag
     1681 ggagaatatc gtggtatcgg gagacttctt gtcgaaaaac atctgggaaa tactgaggct
     1741 cccaataagg ccagaaatgc gctccgagcg actgcgttcc gtagaatgta accagagtca
     1801 gcttcggacg cattgacgtc aacaccgctg gggccaggac tgcgtagaat cggtcgcgat
     1861 ccacattatc cattccttca agattctctt tgtaaggtcc tggatagtcc tccagagcct
     1921 gccaggcggc atcgagtttg attcccaggt cggcatcagg aagatcccaa taaagcgtgg
     1981 atggatattc cgtcacatcc cgaactgttc ccgcacgccg gtctgttgaa aactcttcaa
     2041 agaactgatc gctgatgaca aatccaggaa ccatctccgc gggcgttg
//
LOCUS       DAOQUC010000739         2088 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_67628_length_2088_cov_2.818495, whole genome shotgun sequence.
ACCESSION   DAOQUC010000739 DAOQUC010000000
VERSION     DAOQUC010000739.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67628_length_2088_cov_2.818495"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(74..1618)
                     /locus_tag="PKH07_12100"
     CDS             complement(74..1618)
                     /locus_tag="PKH07_12100"
                     /inference="COORDINATES: protein motif:HMM:NF015585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HNT35728.1"
                     /translation="MVKSCGLTFMAGFLVLTAWLAVFPSGSWAVVRLPKIFGDHMVLQ
                     RNATLPLWGWADAGEEIIVSIGEQRVTTTADSSGQWKVSLAALREPGPYSMTVTGANN
                     SVQFSDILVGEVWFCSGQSNMEWQMNNINQADAEKAAAQFPNIRLFHVPKKAVSHPES
                     DVDASWKACTPEEIPTFSAVAYFFGREIHEKVKVPVGLINCSWGGTRIEPWTPPVGFD
                     GIREVADILTVVQQADSQHKQAVNEALKQYDQWLASAKETFAKGSDVEAPPAWPAHPL
                     SSHVAPTSLYNGMVHPLVPFAIRGAIWYQGESNHQEGLLYTWKMKALVEGWRKVWGQG
                     DFPFYYVQLAPYRYQDPNFLPLIWEAQQKAMDVISKSGMAVVTDLVDNVADIHPRNKK
                     DVGKRLALWALKNEYGQSDLPASGPVYQKMEIEGYRIRLHFTSAEGGLKTRDGKPLDS
                     FMIAGEDKQFVPAMADFEGENIISVASESIVQPVAVRFGWNQEAMPNLINGAGLPASP
                     FRTDNW"
     gene            complement(1743..>2088)
                     /locus_tag="PKH07_12105"
     CDS             complement(1743..>2088)
                     /locus_tag="PKH07_12105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35729.1"
                     /translation="VSGGLFQGITSGNDGRMISLPSLGLTASDWNFVEVRMKVTAGSV
                     VDLFFRSAGDSSFTLGKRIRFDITNTDEFVTYRADTRLVGSWVGDIAQIRFDPSNVSG
                     AQVQVDCIRLLR"
BASE COUNT          519 a          614 c          499 g          456 t
ORIGIN      
        1 gatggtttct ttctcgaacg agcgccttgc agggatgggt atagcccacc atgactgcaa
       61 gcactgtcag agctcaccag ttgtcagttc ggaaaggtga ggcgggaagt cccgcaccgt
      121 tgatcagatt tggcatcgcc tcctggttcc acccaaaacg aacggcaaca ggttggacga
      181 tggattcact ggcaacgctg ataatgttct cgccctcaaa gtctgccatc gccggaacaa
      241 actgcttgtc ctctccggcg atcatgaaac tatccagagg cttcccatct cgggtcttca
      301 gaccaccttc cgcggaagta aagtgaagac ggatcctgta accttcgatc tccattttct
      361 gatagacggg accggaagca ggcagatcgc tttggccgta ttcattcttg agagcccaca
      421 gcgccagtcg ctttccaaca tcctttttgt tcctcggatg aatgtctgca acgttgtcaa
      481 ccaagtctgt cacaaccgcc atgccactct tcgagattac atccatcgcc ttctgttgcg
      541 cttcccagat cagaggcaga aagttaggat cctgatagcg atagggagcc agttgaacgt
      601 agtaaaaggg gaagtctccc tgaccccaga cttttctcca cccctccaca agcgccttca
      661 tcttccaagt gtagagcagt ccttcctggt ggttggactc tccctggtac caaatggctc
      721 cacggatggc gaaaggcacc agaggatgaa ccatgccatt gtagagacta gtcggcgcaa
      781 catgagaact caacggatga gcaggccaag cgggcggagc ctcaacgtcc gaccccttgg
      841 caaaagtttc cttggcagat gccaaccact gatcatactg cttgagcgct tcattgacgg
      901 cttgtttgtg ctgggaatcc gcctgctgta caacagtcag gatatccgcc acttcgcgta
      961 tcccgtcgaa gcccactggg ggcgtccaag gctcgattcg agtcccaccc catgagcagt
     1021 ttatcaagcc gaccggaacc ttgaccttct cgtgtatttc tcgtccaaag aaataggcaa
     1081 ccgcagaaaa cgttggaatt tcctcagggg tgcaggcttt ccaactggcg tcaacgtcac
     1141 tctccggatg actgactgcc ttcttcggaa catggaacag gcggatattc ggaaactggg
     1201 cagctgcttt ctccgcgtca gcctggttga tattgttcat ctgccactcc atattggact
     1261 gtcctgaaca gaaccagacc tcacccacaa ggatgtcgga aaactggact gagttgttgg
     1321 cacccgtcac cgtcattgag tagggtccag gctcacgcaa ggcggccaga ctgactttcc
     1381 attggccaga actgtccgct gtagttgtaa cccgctgctc cccgatagac acgatgatct
     1441 cttcccctgc gtctgcccaa ccccagagag gcagtgtggc gttgcgctga agtaccatgt
     1501 gatccccaaa aatcttgggc aatctcacca ctgcccagga accagaagga aatacagcca
     1561 gccaagccgt cagaaccaaa aaacccgcca tgaaagtcaa accacaactc tttaccatgc
     1621 gaaaatcctc ccttgttcca ggacaccttc cgaatgctta ctggaacggc tatttccctg
     1681 aaacatagca cagaaacaag agggggaaca agattcgtaa aacgggcacg atgctgacac
     1741 ggctaccgaa gaagcctgat gcagtctacc tgaacttgag cacctgaaac gttgctggga
     1801 tcaaagcgaa tctgggcgat gtctcccacc caactgccca caagtcgggt gtcggctcga
     1861 tacgtaacaa actcatccgt gttcgtgatg tcaaaccgaa ttctcttccc caaagtaaac
     1921 gaactgtctc cagcggaacg gaagaacaag tcaacaacac ttcccgcagt caccttcata
     1981 cggacttcaa cgaagttcca atcagatgcc gtcagtccga gactgggcag cgagatcatt
     2041 ctgccgtcgt tgccggacgt tatcccttga aacagcccac ccgacacc
//
LOCUS       DAOQUC010000740         2087 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_67691_length_2087_cov_2.354331, whole genome shotgun sequence.
ACCESSION   DAOQUC010000740 DAOQUC010000000
VERSION     DAOQUC010000740.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2087
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67691_length_2087_cov_2.354331"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            98..1300
                     /locus_tag="PKH07_12110"
     CDS             98..1300
                     /locus_tag="PKH07_12110"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013924960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HNT35730.1"
                     /translation="MNAVSTKPIETLPELVLKPNKDKAIRNLHHWVFSGAVASLSPKT
                     PDGGFVSVWSHGKEFLGIAYVNRRASILARMIAFEPVVPLEAISRSMDEAIALRKRLI
                     DDEEISAYRLINAEGDRLPGLTVDRYNDTLVVQLGTLGMDIVRDEIAGLLSTKLHPLT
                     ILERSDSASRREEGRSNKEGLLQGEPVQQMTIRENGMLFHVDPLKGQKTGFYLDQREM
                     RKLVRRWASGRKVLNCFAYTGGFSVAAVMGGALRVDSVDSSTSALELARENLALNGVN
                     PGDHGFVVDDVFRFLRESKETYGMVILDPPAFAKRKTDIISACRGYKDINRLALEKME
                     SGGLLLTCSCSYHVDEKLFQQVVFEASVEAGRKARIIQHHHHAVDHPVNIHHPESHYL
                     KSLLLYVE"
     gene            complement(1313..2023)
                     /locus_tag="PKH07_12115"
     CDS             complement(1313..2023)
                     /locus_tag="PKH07_12115"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNT35731.1"
                     /translation="MHTSAPHAIALIDATLPDWLETNKGCYTFIWVGDLWERATFMRG
                     MENLLLDVILKPDLVKRLLRGITDYILRTMEILFARFEFDGIALSDDYGTQKAMLISP
                     EEWRRFVRPMLKEIYALAKRHQRTVFHHTCGNVFPIIRDLINIGLDVLHPIQPEAMDI
                     EKLKREFGQDLTFCGGVRTQDLLPYGTPEDIREEVRRLKRTMGNGGGYILEPGISLQV
                     DVPVENLVALIDEARKTD"
BASE COUNT          474 a          537 c          571 g          505 t
ORIGIN      
        1 caatctctcg tcccagttgc tcggttcttg ctttgcctct atcgtcctct gcgacgatgt
       61 caggaatagc cttctatcgt cagaacggat gtagactatg aatgcagttt cgaccaagcc
      121 cattgaaacc cttcccgaac ttgttctgaa gccgaacaag gacaaagcga tacggaactt
      181 gcaccactgg gtgttctcag gagcggttgc atcactttcg ccaaaaacac cggatggagg
      241 atttgtctcc gtctggtcgc atggaaagga gtttcttggg atcgcctacg tcaaccgtcg
      301 ggcttcgatt ttggcacgca tgattgcttt cgaaccggtc gtgcctcttg aggcgatttc
      361 acgctccatg gacgaggcaa ttgcgctcag gaagaggctt attgatgatg aggagatttc
      421 tgcctaccga ctgatcaacg ccgaagggga tcggttgcct ggtttgacgg ttgaccgtta
      481 caacgacacg cttgtcgtcc aactgggaac gcttggcatg gatattgtcc gagacgagat
      541 tgccggactg ctttccacca agttgcatcc actgacgatt cttgaacgct cagactccgc
      601 ttctcgtcgg gaagagggac gctcaaacaa agaaggtctt ctccaaggag aacctgtgca
      661 acagatgacc atccgcgaga atggtatgtt gttccatgta gatcccctta agggtcagaa
      721 gacaggtttt tatttggatc aacgcgagat gagaaaactg gtgaggcggt gggcatcagg
      781 caggaaggtg ctcaactgct ttgcctacac aggtggtttc tctgtcgcgg cagtgatggg
      841 tggagcactt cgagtggatt cggttgactc atccacttcc gccttggaac tggcacgaga
      901 gaaccttgcc ttgaacggtg tgaatccagg ggatcacggt tttgtcgtgg acgatgtctt
      961 tcggtttctg cgcgagtcga aggagaccta tgggatggtg attcttgatc cccctgcctt
     1021 tgccaagaga aagaccgaca tcatctcagc ctgccgtggg tacaaggata tcaatcgtct
     1081 ggcactggag aagatggaat cgggggggct gctcctgacg tgttcctgtt cctatcatgt
     1141 ggatgagaaa ctcttccagc aggttgtgtt cgaggcttcg gttgaggcag gacgcaaagc
     1201 aaggataatc cagcatcatc accacgcagt ggatcatccg gtcaacatcc atcatccgga
     1261 gagccactat ctgaagagcc ttttgcttta tgtggagtga aacaagccgc gcttagtcag
     1321 tctttctggc ttcatcaatc agggcgacga ggttttcaac cggaacgtca acctgaagtg
     1381 agatcccagg ttcgaggatg tacccccctc cgtttcccat cgtccgcttc aaacgtcgaa
     1441 cttcctcacg aatatcctcc ggcgttccat agggaagcaa gtcctgcgtt cgcacacccc
     1501 cgcagaaggt cagatcctgc ccgaattccc gcttgagttt ttcgatgtcc atggcttcag
     1561 gctgaatcgg atgcagaaca tccaaaccga tgttgatcag atctcggatg atcggaaaca
     1621 cattgccaca ggtatgatga aataccgttc tttggtgtcg tttggcgagc gcatagatct
     1681 ccttcagcat cggtcggaca aaacgccgcc actcttcggg tgaaatcagc atcgcctttt
     1741 gtgttccata atcgtcgctg agggcgatgc cgtcaaattc aaatcgcgca aagagaatct
     1801 ccatggttct caggatgtag tcagtgatgc ctcggagcag gcgtttgacc aaatccggct
     1861 tcaagatcac atccaggagc agattctcca tcccgcgcat gaacgtggct cgctcccaga
     1921 gatcgccgac ccagatgaac gtgtagcagc ccttgtttgt ctccagccaa tcgggcaggg
     1981 tggcgtcaat aagggcgatg gcgtgggggg cggaagtgtg catgggcagt ctgaatgaat
     2041 gattcgatga tcaagccccc ggcgcgccaa cgtgcgcgca aaaggct
//
LOCUS       DAOQUC010000741         2085 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_67771_length_2085_cov_3.167980, whole genome shotgun sequence.
ACCESSION   DAOQUC010000741 DAOQUC010000000
VERSION     DAOQUC010000741.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67771_length_2085_cov_3.167980"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="PKH07_12120"
     CDS             complement(<1..288)
                     /locus_tag="PKH07_12120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35732.1"
                     /translation="MMRKTLTLVCCLSGILMCGCAAKFNEWGILGDNLVEPVGNLMPT
                     GLRREGSSVEQASIQMKEDFFICKGTQGKGWSEAETDFTTDDPKLVAVAQLS"
     gene            complement(335..1261)
                     /locus_tag="PKH07_12125"
     CDS             complement(335..1261)
                     /locus_tag="PKH07_12125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020384200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNT35733.1"
                     /translation="MKTLVTGGAGFIGSNLVDGLLQAGHQVVVVDDLSTGSRENVSKQ
                     AAFCQETILNKDGLQAIFSREKPDFVCHYAAQIDVRKSVSDPQHDAAVNIIGGLNVLQ
                     CSLKVGVRKLVYVSTGGAVYGDPAYIPVDENHPVRPLCPYGVSKHALEHYLHLHAVND
                     GLRYTVLRYPNVYGPRQNPKGEAGVIAIFTDKMMQGQRPTIFGDGTQTRDYTFVGDVV
                     AANLLALEKGESEIFNIGTGVESTVLDIVETLNDLLGTHIEPILAPARVGEVYRIALS
                     AERAKSVLGWHPKHDLKAGLKKTVDYYKSVSQ"
     gene            complement(1364..1660)
                     /locus_tag="PKH07_12130"
     CDS             complement(1364..1660)
                     /locus_tag="PKH07_12130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35734.1"
                     /translation="MAQPDDKTCPLDPRRSFVIKKDQMQVIALTDRFRVEGTAHFIPQ
                     ARISDFMNRADIHFIPLTDATLSDLKTGERIYAASFFCLNKSDVVVMIPKDDLA"
     gene            complement(1710..>2085)
                     /gene="rho"
                     /locus_tag="PKH07_12135"
     CDS             complement(1710..>2085)
                     /gene="rho"
                     /locus_tag="PKH07_12135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015681801.1"
                     /note="An RNA-DNA helicase that actively releases nascent
                     mRNAs from paused transcription complexes; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HNT35735.1"
                     /translation="KPKRFFGAARNIEEGGSLSIIGTALIDTGSRMDEVIFEEFKGTG
                     NMELWLDRKLADRRIYPAIDVNRSGTRKEELLLEQDELPRVWLLRKVMNEMNISEVME
                     LIIERMKKTRSNKEFLATLGKS"
BASE COUNT          474 a          609 c          504 g          498 t
ORIGIN      
        1 gctcagctgt gccaccgcca ccagtttggg atcatctgta gtgaaatcag tctcggcttc
       61 actccaaccc ttcccttgtg ttcctttgca gatgaagaaa tcctccttca tctgaatcga
      121 agcctgctca acggaagagc cttccctgcg aagaccagtc ggcatcagat tccccacagg
      181 ttcaaccaga ttgtctccca gaatgcccca ctcattgaac ttggcggcgc atccacacat
      241 cagaatgcca gacagacagc agaccagtgt cagcgttttt ctcatcatca gcccgtgcct
      301 cccaatcgtt tttgactcac tcccagaaca tttgtcactg agaaaccgat ttgtagtagt
      361 ccacggtctt cttcagacct gccttgagat cgtgcttcgg atgccagccc agaaccgact
      421 tggcgcgctc cgcgctcaaa gcaattcggt aaacttcccc cactcgagct ggggcaagga
      481 tcggttcgat atgtgttccc agaagatcat tgagtgtttc gacaatgtcc agaacggtgg
      541 actcgacacc cgttccgata ttgaatatct cgctctcccc cttctccaaa gccaacagat
      601 tcgccgccac gacatccccg acaaaggtgt agtcccgtgt ctgggtaccg tcgccaaaga
      661 tggtgggacg ttgaccctgc atcatcttgt cagtgaaaat cgcgatcaca cctgcttctc
      721 ccttggggtt ttgcctcggt ccgtagacgt tggggtagcg aagcacggtg tagcgcagcc
      781 cgtcattgac cgcgtgaaga tggagatagt gctccaaggc atgtttggac acgccatagg
      841 gacacagagg acgaaccgga tggttctcat ccactggaat gtaagcggga tctccataga
      901 ctgctccacc ggtcgagacg tagaccaatt tcctgacgcc gaccttcagg ctacactgaa
      961 ggacgttcag tccgccaatg atattcacgg ctgcgtcatg ttgaggatct gagacggatt
     1021 ttcgaacgtc aatttgagcg gcgtagtggc agacgaaatc cggtttctcg cggctaaata
     1081 tcgcctgaag tccgtccttg ttcagtatcg tttcctgaca aaaggcggcc tgcttcgaaa
     1141 cattttcgcg gctgccggta gacaggtcat ccacaaccac cacctggtgt cctgcctgca
     1201 aaagcccatc cacgaggttg gagccgatga atccggctcc gcccgttacc aaagtcttca
     1261 tttgtcacct cagactacga gcaacagttg ccaacagctg cccttctgtc actgtttcca
     1321 caaacaggga atgctggaaa tgggactgcc gaccgcgaga cgttcaagcc agatcgtcct
     1381 ttggaatcat gaccaccaca tccgacttgt tcagacagaa gaaactggct gcgtaaatcc
     1441 tctctcctgt cttcagatcc gaaagcgtcg catccgtcaa aggaataaag tgaatgtccg
     1501 ctcggttcat gaagtcggag atgcgcgcct gtgggataaa gtgtgccgtc ccttccacac
     1561 gaaagcgatc ggtcagggca atcacctgca tctgatcctt cttgatgaca aaactgcgtc
     1621 gaggatcgag agggcaggtc ttgtcgtcgg gttgcgccat ggcagtagcc tccttcgcgg
     1681 cgataccaac atacttccct gatcacatat cacgacttgc ccagagtggc aagaaattcc
     1741 ttattactgc gcgttttctt cattcgctcg atgatcagtt ccataacctc gctgatgttc
     1801 atctcgttca tgaccttgcg aagcaaccag acgcggggca gttcgtcctg ctccagcagg
     1861 agttcttcct ttcgggttcc agaacggttc acatcaatag cgggatagat gcgcctgtcc
     1921 gccagtttgc gatccagcca gagttccatg ttgccagtac ccttgaactc ttcgaagatg
     1981 acctcgtcca tacggctgcc tgtatcaatg agcgccgttc caatgatgga gaggcttcca
     2041 ccttcctcga tgttacgcgc agctccaaag aaacgcttgg gctta
//
LOCUS       DAOQUC010000742         2085 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_67789_length_2085_cov_2.491133, whole genome shotgun sequence.
ACCESSION   DAOQUC010000742 DAOQUC010000000
VERSION     DAOQUC010000742.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67789_length_2085_cov_2.491133"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(22..849)
                     /locus_tag="PKH07_12140"
     CDS             complement(22..849)
                     /locus_tag="PKH07_12140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35736.1"
                     /translation="MNTTARPNGFRSRSVMIVIAVLFFLVESHAFAQQHPAPEFTVGI
                     ELPPQIPIAPAVESALHDMGITFMNYYVKPDLWTPDETAVRINKEMLDLVQRLDVDFA
                     IACYSTDPPESCVKEAVQRGKVDEGVTRFKGVIFDELEHCRLIYNHAPTPLADTSHFT
                     TLSEAYEGTLEGYRNLKAVHASNSAPAIATHIWPVLLHVAARAGFTPCPKICKELYSS
                     VSLAIGMGAALQYGTEFWANCDLWFWDMIPGHPADEFKSNLLLAYWLGVVRLYVEGC"
     gene            complement(859..1890)
                     /gene="waaF"
                     /locus_tag="PKH07_12145"
     CDS             complement(859..1890)
                     /gene="waaF"
                     /locus_tag="PKH07_12145"
                     /inference="COORDINATES: protein motif:HMM:TIGR02195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HNT35737.1"
                     /translation="MKNIVVRATNWVGDAVMNTPAVLAVRTSFPNARITILAKPWVAG
                     VFENNPSVHSIIHLAKQDLNFPLGPWHLGRRLKREDFQRSYILPNSFSSALSMSLARI
                     PERIGYALQHRSLLLTQPVRVTPELLSCHQVFYYLGLIPEAQLTPDECHPRLYLSQQE
                     RSDAESLLASEGIAPNETLIGVGPGAVYGPAKRWPEERFASVIKRLAGDGNRRFLLFG
                     SPSERSIAEKVASLSGVVCVNFAGKTTLRELISLLERCCIFLTNDSGAMHIAAALRVP
                     VVAVFGSTNPVTTSPYGDGHLLIRHPVPCSPCLKRECPTDFRCMLEITPEEVAEACEL
                     QLHRRKSTE"
BASE COUNT          525 a          538 c          566 g          456 t
ORIGIN      
        1 atcggcgggg cggagattgt atcagcatcc ttcaacgtaa agacgaacca caccaagcca
       61 gtaggcgaga aggagattgc ttttgaactc gtccgccgga tgtcccggga tcatgtccca
      121 gaaccacaag tcgcaattcg cccagaactc cgttccgtac tgtaaggcgg cacccatacc
      181 tatggcgagg ctgaccgatg aatacagttc cttgcaaatc tttggacaag gagtgaaacc
      241 cgcccgggcg gcaacgtgca agagaacagg ccagatatga gtcgcaatcg ctggagcact
      301 gtttgaggca tggactgcct tgaggttccg atatccttcg agtgtgcctt cgtacgcttc
      361 tgaaagcgtg gtgaagtggc tggtgtctgc cagaggtgtt ggagcatggt tatagatgag
      421 gcgacagtgc tccaattcat caaagatgac acctttgaat cgagtgacgc cttcatcaac
      481 cttgccacgt tgcaccgctt ctttgacaca ggattcagga ggatccgtcg aataacaggc
      541 aatggcaaag tcaacgtcca gccgttggac taaatccaac atctctttgt tgatccgaac
      601 ggcagtttcg tcaggagtcc acaagtcagg tttgacgtag tagttcataa aagtgatgcc
      661 catgtcgtga agagcacttt cgaccgctgg agctatggga atctgcggtg gcagctcaat
      721 gcccacagtg aactcaggcg caggatgctg ctgtgcgaaa gcatgggact cgacaaggaa
      781 gaaaagtaca gcaatcacga tcatcacaga tcgagaacga aagccattcg gacgtgctgt
      841 cgtattcatg gttgtgcctc attctgtaga ctttcgtctg tgcaactgta actcacaggc
      901 ctctgccact tcttccggag taatctccag catgcaccgg aaatccgttg gacattcacg
      961 tttcagacaa gggctgcacg gaactggatg tcgaatcaga aggtgcccgt cgccataggg
     1021 tgacgtggtc acaggattgg tcgagccaaa cactgcgacg acgggaactc gaagcgcggc
     1081 ggcaatatgc attgctccgg aatcatttgt caggaaaatg caacatcgct caagcaatga
     1141 gatcagctcc cgaagcgttg tcttccctgc gaaattgacg catacgaccc cggaaagaga
     1201 ggcgaccttc tcggcgatgg agcgctctga ggggctccca aacaggagaa agcgccgatt
     1261 cccatctcct gcgagcctct taatgacgga agcgaaccgc tcttccggcc atcgtttagc
     1321 gggaccatag acggctccag gacctacgcc gataagggtc tcattgggtg caatgccttc
     1381 agacgccaag aggctctcag cgtctgagcg ctcctgttgg ctgagataca acctcggatg
     1441 gcattcgtcg ggagtcaact gtgcttcagg aatcaatcca aggtagtaga atacctggtg
     1501 acaggaaagc agttccggcg tcacacgaac aggctgcgtc agcagtagac ttctgtgctg
     1561 aagtgcgtag ccgatgcgct caggaatccg cgcgagagac atggacagcg ctgaggaaaa
     1621 ggagttcggc aggatgtaag agcgttggaa atcctcgcgc ttgagcctcc ttccaagatg
     1681 ccaaggtccc aaaggaaagt tcaagtcttg cttcgcaaga tgaatgatgg agtggacgct
     1741 gggattgttc tcaaagactc ccgccaccca aggctttgca agaatcgtaa tccgggcatt
     1801 gggaaatgac gtgcgaacag ccaggactgc gggcgtgttc atgactgcgt caccaaccca
     1861 gttggtcgca cggacaacaa tgttcttcac gtcattcatc gagggaaaaa gttacagact
     1921 ccctgccgga ctgtcaaatc ctgctctgtc ttcacgtttt cacatcttga catcggttct
     1981 gccgttatcc taaacttcag tcagcgttca ttgaaaacac ctgtttatat tgcttcgctg
     2041 caaagatcgc cgttggcaag aggagcgtcc tatgattacc aaagc
//
LOCUS       DAOQUC010000743         2084 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_67836_length_2084_cov_2.863972, whole genome shotgun sequence.
ACCESSION   DAOQUC010000743 DAOQUC010000000
VERSION     DAOQUC010000743.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67836_length_2084_cov_2.863972"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..895)
                     /locus_tag="PKH07_12150"
     CDS             complement(<1..895)
                     /locus_tag="PKH07_12150"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNT35738.1"
                     /translation="MQFEPFLPPWILIAGFFLSAAVVFLSYWRRLRQQESLLVYVFLL
                     FKIGALVVLVFMLARPVTQTRRKEKVHTVLPVLLDASESMGVEDQAGTTRFNKARDLI
                     LYHLSKQLSEDFDLRLYTFGSFLEPQTEGTLAAIPAAYGSSTDIGGAVIESLRREGKR
                     VDALVLLSDGVHNLGTHPAEITPALKQQGVRVYTVGIGQAGDYKDVEIGSISVPTMVF
                     MNEEVPVTFDIRSYGYKSVSAEVILQGTLVDETTAKVVQSDTTAAAKGNTMVLARQTV
                     ELTEKFPQERGKITFTIGERGT"
     gene            complement(928..1899)
                     /locus_tag="PKH07_12155"
     CDS             complement(928..1899)
                     /locus_tag="PKH07_12155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NGG1p interacting factor NIF3"
                     /protein_id="HNT35739.1"
                     /translation="MNLEQFFAKAVQVGIDQDPRGRKAVEKLLKQTQQEYDKLDEKKK
                     GYFDTDSLTNPFADSRFLFGEPETPLKRMLVGIDIEGPEVLLADRLRERGEGFDLVLG
                     HHPEGIGLTSLHKVMYVQADVWHSIGVPISVAESLIGKRASEIEKRLLASNCNRAVDL
                     ARLLNIPLMCTHTPADNCVTKYLQKRMDAEKPEKVSDVIDFLLTQPEYQDSSRLGTGP
                     KVILGSESRRAGRIMVDMTGGTGGPKEILERIAQTDVGTIVGMHMSEDCYKEAEKNHL
                     NVVIAGHIASDNLGMNLLLDMTLGKAPVEVVECSGFKRVRAKERKTV"
BASE COUNT          447 a          595 c          522 g          520 t
ORIGIN      
        1 aggtgcccct ttccccgatg gtgaaagtga tcttcccgcg ctcttgggga aacttctctg
       61 taagttccac cgtctgacgc gcaagcacca tcgtgttgcc cttagcagcc gctgtcgtgt
      121 cactctggac gactttcgcg gtcgtttcgt ccacaagcgt tccctgcagt atcacttcag
      181 cactgactga tttgtatcca taggatcgaa tatcgaatgt tacggggact tcttcattca
      241 tgaaaaccat agtcggaaca gaaatgctcc caatctccac gtccttgtag tcacccgcct
      301 gaccgattcc aaccgtgtag acgcgcaccc cctgttgctt gagagctggg gtgatctctg
      361 cggggtgggt tcccaggttg tgaacgccat cgctcaaaag cacgagtgcg tccacccgtt
      421 tgccctcacg acgcagggac tcgatcactg ccccaccgat atctgttgaa cttccatagg
      481 ccgcagggat ggcggcaagt gtcccttcgg tctgaggctc aagaaaagat ccgaatgtgt
      541 acagccgcag atcgaaatcc tcggacaatt gctttgacaa gtgatagagg atgagatcgc
      601 gagccttgtt gaagcgcgtt gtccccgctt gatcctccac gcccatgctt tcggaagcat
      661 ccagcaagac ggggagtacg gtgtgaactt tctcttttct acgcgtctgt gtcacagggc
      721 gggcaagcat aaagactaag accacgagag cgcctatctt gaacagcaga aagacataga
      781 cgagcagcga ctcctgttgc ctcagccgcc gccagtagga aagaaacacg acagcggcgg
      841 agagaaagaa tcccgcgatc aaaatccacg gtggaagaaa tggctcaaac tgcacgccca
      901 gaagcacccc ctaccggacg attccggtca aacagtcttt ctctctttgg ctcttaccct
      961 cttgaatccg gagcactcaa cgacctcaac tggagccttt cccagagtca tgtcaagcaa
     1021 cagattcatc cccaggttgt cactggcgat atgaccggcg atgaccacgt tcagatgatt
     1081 tttctccgct tctttgtagc agtcctcgga catgtgcatt ccgacgatcg tgccaacatc
     1141 cgtttgtgcg atacgttcca gtatctcttt aggcccaccg gttccacctg tcatatccac
     1201 catgatcctg ccggctctgc gcgattccga tccgaggatg acttttggtc ctgtgccaag
     1261 tcgcgacgag tcctggtact cgggctgtgt gagaagaaaa tcaatcacat cggagacctt
     1321 ctctggcttt tccgcatcca tccttttctg tagatacttg gtgacacagt tgtctgcagg
     1381 tgtgtgtgta cacatcaacg gaatgttcag aagacgggct agatccacag cacggttgca
     1441 gttgctggcc agaagtcgct tttcgatttc gctggcgcgt ttcccgataa ggctttcggc
     1501 aacgctgatc gggacaccga tgctgtgcca gacatctgcc tgaacgtaca taaccttgtg
     1561 aaggctcgtc aggccgatcc cctccggatg gtgtcccaga accaggtcga acccttcgcc
     1621 cctttctctg aggcggtctg ccaggagcac ttcgggaccc tcgatgtcaa tgcccacgag
     1681 cattctcttg agtggggtct ccggctctcc gaagagaaaa cggctgtcgg caaaggggtt
     1741 tgtcaaactg tcggtatcga aatacccttt cttcttctcg tcgagtttgt cgtactcttg
     1801 ttgtgtctgt ttgagaagtt tctcaacagc ctttcttccg cgcggatcct gatcgatccc
     1861 aacttggact gcctttgcaa agaattgttc cagattcatc gtacgtactc cttgtgctcg
     1921 ctggcaagat ctatggctgc gccttgacaa tcaaggcgat gtctttgtcg aaggatggca
     1981 gaacaaccct gacttttcct gaatccgctt tgacgacgct ttgctggatt ttcttgcctg
     2041 tgcgggtgtc gaaaacccgc acctgaaaac tgccttccga ggag
//
LOCUS       DAOQUC010000744         2082 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_67924_length_2082_cov_3.640849, whole genome shotgun sequence.
ACCESSION   DAOQUC010000744 DAOQUC010000000
VERSION     DAOQUC010000744.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2082
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67924_length_2082_cov_3.640849"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..655
                     /locus_tag="PKH07_12160"
     CDS             <1..655
                     /locus_tag="PKH07_12160"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNT35740.1"
                     /translation="LSIIHAHKGALRWKIETEGVPAHSSTPEKGINAIYKMRRVLECI
                     EQMAVPALNTKSHTLLGKPTLSVGTIAGGSQVNVVPGNCVIEVDRRTIPGESEQRLTE
                     ELLGHLDQVRALDPDLKFKIEPIEWYPSFEERTDSPVASLVQSACDRVLGTSRFNVAP
                     WAANSGVFKAAGIPCVLFGPGSVAQAHTREEYIELDQVEKAVGVYAEIIRNAGAVIS"
     gene            complement(645..>2082)
                     /locus_tag="PKH07_12165"
     CDS             complement(645..>2082)
                     /locus_tag="PKH07_12165"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HNT35741.1"
                     /translation="RLEQAKPFRPDHLIDAARSYSGLRSVTLDGKAVKLNRKTLFQRL
                     VLDQGYYPDGIMTAPSDAALKHDIELSMAAGFNGARLHQKVFEPRFLYWADRLGYLVW
                     GEFPNWGMDYARKEACLTFVDEWTEIVNRDRNHPSIIGWCPFNETPHTASPLQKTVYH
                     ITRSLDPTRPVLETSGWVHSLADPQLLDAHDYNQNPTSFRTVYGTDSDLQVPERYGVS
                     MARRDRPFFISEFGGIGWNLQDGWGYGSSPKTEEEFYTRYEGLTNVLLDNRGMFGFCY
                     TQLTDVEQEQNGLYRYDRTPKFDAKRIQRITSRRARYEEDPPLNTTPSDVEWCVLVGA
                     AVDGDRAQTWRYQMETQPKEGWETVDYDDSSWQESKGGFGDKGGWESRTGTTWKGKDI
                     WLRGQFEHDGSSFDRAVFVTHYDNATEIYVNGELIWEGEGWNDAYAPFEITDTLKRAL
                     KRGTNTIAVHCHQDTGGQFIDLAVLVTK"
BASE COUNT          467 a          576 c          555 g          484 t
ORIGIN      
        1 tctttcgatc atccatgcgc acaaaggagc cttgcgctgg aagatcgaaa cggaaggagt
       61 tccggcacat agttcaactc cagagaaggg aatcaatgcc atctacaaaa tgcgtcgtgt
      121 tctggaatgc attgagcaga tggctgttcc cgctctcaac accaaaagcc acacactgct
      181 tgggaagccg actttgagcg tcggaaccat cgccggtggc agccaggtca acgtggttcc
      241 cggcaactgt gtcattgaag tggacaggcg aacgattcca ggagaatccg aacaacggct
      301 gacagaagag ttacttggtc acctggatca ggttcgagca ctggatcccg atctcaagtt
      361 caagatcgaa cccattgagt ggtatccctc ttttgaagaa agaacggact cccccgtagc
      421 cagtctggtg cagtccgcgt gcgatcgggt attgggcaca agccgattca atgtggctcc
      481 ctgggctgcc aactccggtg tgttcaaggc agcgggtatt ccatgtgtgc tctttggtcc
      541 cggcagtgtg gcgcaggcac acacgcgcga ggagtacatc gaactcgacc aggttgaaaa
      601 ggccgttggt gtctatgcag agatcatccg aaatgccggt gcggtcattt cgtgaccagc
      661 accgccagat caatgaactg cccgccagta tcctgatggc aatggacggc gatcgtgtta
      721 gtgcctcgct tgagtgcacg tttgagcgta tcggttatct caaaaggcgc ataggcgtcg
      781 ttccagccct cgccctccca gatcagttct ccgttcacat agatttccgt cgcgttgtcg
      841 taatgcgtga cgaacacggc tctgtcgaat gatgaaccgt cgtgctcgaa ctgtcctcga
      901 agccagatat ctttgccctt ccaggtcgtt cccgtgcggg attcccatcc acccttgtca
      961 ccaaagccac ctttgctttc ctgccaggaa ctgtcgtcat agtcaacggt ttcccatccc
     1021 tctttgggtt gggtctccat ctggtagcgc catgtctgag cacggtcgcc atcaacagct
     1081 gcaccaacca aaacacacca ctctacatcc gaaggagttg tgttcagcgg tggatcttct
     1141 tcatatcgag cgcgtcttga ggtgattcgt tgaatgcgct tcgcatcgaa cttgggtgtt
     1201 cgatcatatc tgtaaagccc gttctgctcc tgctccacat ccgtcaattg ggtgtaacag
     1261 aagccgaaca ttcctctgtt gtccagaaga acattggtga gaccttcata gcgtgtgtag
     1321 aactcctcct cagtctttgg agaggaaccg tacccccagc cgtcttggag attccaaccg
     1381 attccgccga actcgctgat gaagaacggc cggtcacggc gagccattga gacgccgtat
     1441 cgctctggaa cctgcaaatc agagtctgtt ccgtagacag tccgaaagga tgtgggattc
     1501 tgattgtagt catgggcatc gagaagctgg ggatccgcaa ggctgtgcac ccaaccactg
     1561 gtctccaaca ccggccgcgt cggatcgaga gaacgggtga tgtgataaac cgttttttga
     1621 agtggagatg ccgtatgcgg cgtctcattg aaaggacacc agccgatgat gcttggatga
     1681 ttccgatctc ggttgacgat ttcagtccat tcatccacaa acgtgagaca tgcttccttc
     1741 ctggcatagt ccatacccca gttcggaaac tcaccccaga ccagataacc cagtctgtcc
     1801 gcccagtaaa gaaaccgcgg ttcaaagact ttttgatgca gacgggcgcc gttgaaaccc
     1861 gccgccatcg atagctcaat atcatgcttt aaggccgcat cgctaggcgc ggtcatgatg
     1921 ccgtcaggat agtagccttg atcgagaacc agccgttgaa aaagggtttt tctgttgagt
     1981 ttgaccgctt tcccatcgag ggtcacactg cgtaagccgc tgtaagagcg ggccgcgtcg
     2041 atgaggtgat cggggcggaa cggcttcgcc tgctccagcc gc
//
LOCUS       DAOQUC010000745         2080 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_68032_length_2080_cov_2.932346, whole genome shotgun sequence.
ACCESSION   DAOQUC010000745 DAOQUC010000000
VERSION     DAOQUC010000745.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2080
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68032_length_2080_cov_2.932346"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            237..737
                     /locus_tag="PKH07_12170"
     CDS             237..737
                     /locus_tag="PKH07_12170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HNT35742.1"
                     /translation="MKKILVVYYSRSGNTKKLAEAIVEGAQTIEDVEVTLKSVEQVTN
                     DDLVATDALIAGSPVYYGSMAAPVVDMFAKSVAVRKKMENKLGAAFATSGHHTGGKET
                     TITGILQAMLISGMIVLGDPLKSGGHYGVGCVGSPDEEALTSAQALGSRVASLLTGRT
                     TLGTSR"
     gene            824..1567
                     /locus_tag="PKH07_12175"
     CDS             824..1567
                     /locus_tag="PKH07_12175"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding family 9-like protein"
                     /protein_id="HNT35743.1"
                     /translation="MIALVSLMACSLLTGATVEQNIPWYECLKTTSPPTIDGQLTDAA
                     WADVPEIPHFYESCSGKVAKYLTTARLCWDNNNLYIAFRSEDPCIWGTLTKRDSAIFN
                     EEVVEVFLQPDPKDSIYYEFEVSPRNVKFDSLIRSVTPENRYSGNSKWTCKGLKTAVF
                     VEGTAAEKSESDRFWQVEIAIPFKGLKRTPPKTEEVWRANLYRIERNDNLTEFQAWSP
                     TLTPRPNFHVPARFGRLIFKDLDRASDAN"
     gene            1736..>2080
                     /locus_tag="PKH07_12180"
     CDS             1736..>2080
                     /locus_tag="PKH07_12180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35744.1"
                     /translation="MNACSHTWYEQDLYILADQQLIEKTENVSFNIAKIEKEPDPVIA
                     PDRPWEGYLDDRPPDSLQDPFYATVLYDGSERMFQCWYRPLNRYLSNAYRPPFVNQES
                     RLCYARSRDGRHW"
BASE COUNT          514 a          541 c          540 g          485 t
ORIGIN      
        1 tctgttccga aagggatatg ggacgttaca gaaatcgcca tcggccacgt caaaagcacg
       61 gtcaagacaa ggtagctgag accgatcagg tactggttct tcactgattc tccgtaacgc
      121 ttccgatgac gcaagccaac catgagatga cagacttcga tatcgtattg gcctgacaat
      181 atcttgtcaa ttcctgcaca aaatggtagt aatacagcga gtaggaggtc tgcaaaatga
      241 agaagattct tgttgtttac tattcgcgtt caggcaacac caagaagctc gctgaggcga
      301 ttgttgaagg cgctcaaacg attgaagatg tcgaggtcac tttgaagtcc gtcgagcagg
      361 tgaccaacga tgacttggtt gccaccgatg cactcatcgc aggttctccc gtctactacg
      421 ggagcatggc agctcctgta gtggatatgt tcgcgaagag cgtcgcggtg cgcaagaaga
      481 tggagaacaa actcggcgcc gcttttgcca cttcaggcca tcacacgggc ggcaaagaaa
      541 caacaattac gggtattctt caggcaatgc tcatctccgg gatgatcgtc ctcggcgatc
      601 cactcaagtc tggtggtcac tacggggtcg gctgcgttgg ttcaccggac gaagaagcgc
      661 tgacctcagc acaggccttg ggcagtcgag tcgcctcgtt gttgacgggt cgaacgacgc
      721 tgggtaccag ccgatagata tccgatcatc tgttccggtg gttggaggaa ctgatcgcct
      781 actggcgttt tgtcattgtt gagaacctgc ggaggaatat ggaatgattg cgctggtttc
      841 actcatggcc tgctccttgc tcaccggagc aacagttgag cagaatatcc cttggtatga
      901 atgcttgaag accacttctc cacccactat agacggccag ctaaccgatg ccgcatgggc
      961 tgatgttccg gagatcccgc atttctatga atcctgttcc ggaaaagtgg cgaagtatct
     1021 gaccaccgca aggctctgct gggacaacaa caatctgtac attgcctttc gctccgaaga
     1081 tccatgtatt tgggggacgt tgaccaagcg agattcagca atcttcaacg aagaagttgt
     1141 tgaggtcttt cttcagccag atccgaaaga ctctatctat tatgagttcg aggtaagtcc
     1201 cagaaatgtg aagttcgact ctctgattcg ttcggtgacc ccggaaaatc gctacagtgg
     1261 caactcaaaa tggacgtgca aaggtctgaa gacagcggtg tttgtagaag gcacggcagc
     1321 cgaaaaatca gaatctgacc gattctggca agttgagata gccatccctt ttaagggact
     1381 taagcgcact ccacccaaga ccgaagaggt ttggcgtgcg aatctttatc gaatcgaaag
     1441 aaacgacaat ctcactgaat tccaggcgtg gtcacccact ctcacgccaa gaccgaattt
     1501 ccacgttccc gcacgtttcg gccggctgat cttcaaggat ttggatagag catccgatgc
     1561 aaactgactc gacaggccgt tgtgggagtt ttcctctgta ctcatcgcat ggtccgcagg
     1621 catggatggc aatgggaact ctactccctt ctgctcacac ttcctctcgg cagaaggtgc
     1681 ctcacggcct cgtgtgttct cgctcaactg ccgtgaccaa gcggggtgat tcgagatgaa
     1741 tgcctgctcg cacacttggt atgaacagga tctctatatt ctcgccgatc agcagctgat
     1801 cgagaaaacc gagaacgtct cattcaacat tgcgaagatt gagaaggagc cggatccagt
     1861 catcgcaccg gaccgtccct gggaaggcta cctggacgat cgcccgcctg actccttaca
     1921 agacccattc tatgcaacag tcctctatga cggtagtgag cgaatgtttc aatgctggta
     1981 tcgtcccctg aaccgctatc tgagcaatgc ctatagaccc ccctttgtca accaggaatc
     2041 cagactctgc tatgccagga gcagagacgg acggcactgg
//
LOCUS       DAOQUC010000746         2078 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_68139_length_2078_cov_3.769155, whole genome shotgun sequence.
ACCESSION   DAOQUC010000746 DAOQUC010000000
VERSION     DAOQUC010000746.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68139_length_2078_cov_3.769155"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            333..665
                     /locus_tag="PKH07_12185"
     CDS             333..665
                     /locus_tag="PKH07_12185"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNT35745.1"
                     /translation="MQEESALFKVLSDPIRLRLAILLAMQGETCVCMLARALGEPNFK
                     VSRHLGIMRSAGMVEARREGTWMHYKLVEPRHRLEECLQECFRECLCDHTVIKEDMKR
                     LRKAECGK"
     gene            679..1125
                     /locus_tag="PKH07_12190"
     CDS             679..1125
                     /locus_tag="PKH07_12190"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HNT35746.1"
                     /translation="MTQKTKMLFLCTGNSCRSQMAEGWARHLKSDILEPYSAGIEKHG
                     LNPLAVKAMAEVGVDISGHQSKLVLDLPTMEFDYVVTVCGNAHETCPYFPGDAKIVHV
                     GFDDPPSLAKSAKTEDEALDHYRRVCNEIRAFIETLPEGFERHSEE"
     gene            1137..1469
                     /locus_tag="PKH07_12195"
     CDS             1137..1469
                     /locus_tag="PKH07_12195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35747.1"
                     /translation="MNAQVDIDAGVCGFHTSAVAKSEDGQSVCYEITTDCEKIRNLAQ
                     ALSDVGSIDAYQEISPASESRLLAISRQTLSGCCSGCAVPVGLFKAMQVAAGLALPKD
                     IEIRLSKE"
     gene            1481..>2078
                     /gene="arsM"
                     /locus_tag="PKH07_12200"
     CDS             1481..>2078
                     /gene="arsM"
                     /locus_tag="PKH07_12200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HNT35748.1"
                     /translation="MSSKHEDRDEEVRGIIREQYGKIATQGGSCCCSSSCCGTPPADA
                     VATVVGYSNEDLAGLPAGANMGLSCGNPTAFASLKPGEVVLDLGSGGGFDVFIAARKV
                     GASGRAIGVDMTPDMLDKARRNAVSFQKETGLSNVEFRLGEIEHLPVADSSVDVVISN
                     CVINLSPDKPQVWREIARVLKPGGRVSVSDLALLQPLPD"
BASE COUNT          509 a          474 c          613 g          482 t
ORIGIN      
        1 acagagagaa cagctgcagt cttcgctgtc cagcctcgca aaggaagggt ttgccagaat
       61 ccacgttctg tcatccccag agcagataaa cgatctgtgt atcaagaaac agtttcaggt
      121 agttgagtga gcagagcgag tttgacagca gaacaaagcc tctttggccg aaacatatcg
      181 agatatgttt cgggttggcg tgtcaagtcg atttctgcct tctcgccgcg gcaaaatcaa
      241 gatcagaacc ttttctgaaa aaatagattg aaactcatgt ttgaatccaa tatatgtgta
      301 tatgtgcata tatgcatata aaggaaaaca ggatgcagga agaatctgcc ttgttcaaag
      361 tcttgagcga tcccatcagg cttcgtctgg caatcctcct tgccatgcaa ggagagacct
      421 gtgtatgcat gctggcgaga gctttgggtg agccgaattt caaggtctct cgtcacctgg
      481 ggatcatgcg ctcggctggt atggtcgagg cgaggcgtga gggaacttgg atgcactaca
      541 aactggtcga accgcgacac cgactggaag agtgtcttca ggaatgcttt cgcgagtgcc
      601 tttgcgatca tacggtgatc aaagaagata tgaaacgctt gaggaaggct gagtgtggga
      661 agtgagggac gagacagcat gacgcaaaag accaagatgc tctttctctg caccggcaat
      721 tcctgtcgga gccagatggc tgagggttgg gcacggcacc tgaaaagtga catccttgag
      781 ccgtattcgg cgggcatcga aaaacacgga ctcaatccgc tggcggtcaa ggcaatggct
      841 gaagttggtg tggatatcag cgggcaccaa tcaaaactgg tccttgatct cccgactatg
      901 gagttcgact acgtagtcac cgtttgtggg aatgctcatg agacttgccc atacttccca
      961 ggggacgcaa agattgttca cgttggattt gatgatccac ccagtcttgc caagagtgca
     1021 aagacagagg atgaggcgct cgaccactat cggcgtgtat gtaatgaaat tcgggcattc
     1081 atcgagactt tgccagaagg ctttgagaga cattcagagg aatagaagga gaggaaatga
     1141 acgctcaggt tgacatagac gctggggtgt gtggtttcca cacaagtgct gtcgcgaaaa
     1201 gtgaagacgg tcagtcggtc tgctacgaga tcacaaccga ctgcgagaag attcggaacc
     1261 tggcacaggc gctttcagac gttggcagca ttgatgcgta tcaggagatt tctcctgcaa
     1321 gtgaaagtcg gctccttgcc atctctcgcc agactctgag cggctgctgc tccggatgtg
     1381 cggttcccgt aggtcttttc aaagccatgc aggtcgcggc aggactggcg ctccccaaag
     1441 acattgagat tcgcctgagc aaggaatagg agagagatat atgagttcga aacatgaaga
     1501 tcgagatgaa gaagttcgcg gcatcatccg cgaacaatat ggaaaaattg ccacgcaggg
     1561 tggatcttgc tgctgttcgt catcttgttg cgggactccg ccagcggatg ctgtcgcaac
     1621 ggtggtgggc tacagcaacg aggaccttgc cgggcttcca gcaggagcaa acatggggct
     1681 ttcctgtggc aacccgacgg catttgcgtc gctcaagcca ggagaagtgg tgcttgatct
     1741 tggatcaggt ggtgggtttg acgtgtttat tgctgcgcgc aaagtcggcg cttccggtcg
     1801 tgctatcggc gttgatatga cgccggatat gttggacaag gctcggcgga acgcggtgtc
     1861 ttttcagaag gaaaccggcc tgagcaatgt ggagtttcgt ctgggtgaaa tcgaacatct
     1921 gcctgtggcg gactcaagcg ttgatgtggt catctccaac tgtgtcatca atttgtcgcc
     1981 cgacaaaccg caggtatggc gtgagatcgc ccgtgtcttg aaaccaggcg ggagagtttc
     2041 ggtgtccgat ctggcgctct tgcagccact gcctgatg
//
LOCUS       DAOQUC010000747         2078 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_68147_length_2078_cov_3.061789, whole genome shotgun sequence.
ACCESSION   DAOQUC010000747 DAOQUC010000000
VERSION     DAOQUC010000747.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68147_length_2078_cov_3.061789"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1773)
                     /locus_tag="PKH07_12205"
     CDS             complement(<1..1773)
                     /locus_tag="PKH07_12205"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HNT35749.1"
                     /translation="MTEPHVETRKNGVPADFVRTVMIPVFRARWRWIVFVPFCVAVLT
                     AIYSLIINEEYQSEAKLIVVPPKYREQQSLLPEQFSMKTYSELFNSPDLVQDLLDRLR
                     LSSRAIQAIRKSYPDLDWAHQKKNRDFVAEGFKKADSLTVEEMLRITGCSMDEALFLT
                     GGRSAAGERTYQGLTATDWIGADLFDERELQETKLEEFAKTISVKLVVEEDTPYGTTY
                     APVLTISTKANSAKGVKLKTGILADLFLERARLVTAENTKEAVESVSSAFVQAATDAW
                     GAGNAIREYKANNPIELVQKQLDSLNQVLLGGSEQYRDPTTNELVYQPSALAELKQLE
                     RLIEKKRKSLDQLAHELDELRKPTGEWVGAMRTYLNPDEALQSLAGDIESASRQGKFL
                     LLGVLRSKEGVWRALASLEEFEERTGYEMELSRLTDELINLNTLRKGVVQSQSELKAW
                     EQRLASEESIREGMKSVVELKGQIPLEAITPLLNKVDSSKDVETLGKLTYEQEVFSPE
                     YEELGAKLVNSTGQRAYFKAMNEAQQAALAAKEQEVMELQKKLTLWREQKSILTHAVE
                     EARKAHNQQYIEFIETQTLHQNTEK"
BASE COUNT          484 a          572 c          488 g          534 t
ORIGIN      
        1 tttctcggta ttctgatgaa gggtctgcgt ctcgatgaac tcaatgtatt gctgattgtg
       61 ggctttgcgg gcttcctcaa cggcatgagt gagtatactt ttctgctctc gccacagggt
      121 tagtttcttc tgaagttcca tgacttcctg ctcttttgcg gcgagagcag cctgttgcgc
      181 ttcgttcatc gccttgaaat aagccctctg cccagtagaa ttgaccaact tggctcccag
      241 ttcctcatac tcaggagaga acacttcctg ttcataggtg agtttgccaa gagtctccac
      301 atcctttgag gaatcaacct tgttcaagag aggtgtgatc gcttctaagg gaatctgccc
      361 cttcagttct acaacggact tcattccttc ccgtatggat tcttctgagg caagcctctg
      421 ctcccaagcc ttcaattcgg actgggattg aaccacaccc tttcggagcg tattcaggtt
      481 gataagttcg tcagtgagac gggagagttc catctcgtat cccgttcgtt cttcaaactc
      541 ctccagtgat gcaagcgcac gccagacccc ttccttggag cgcagaacac caagcaagag
      601 gaactttccc tgtctggatg ccgactcgat atcgccagca aggctctgaa gcgcttcatc
      661 tggattcaga taggtacgca tcgctccaac ccattctccc gtgggctttc tcaactcatc
      721 caattcgtgt gccagttgat caaggctctt gcgtttcttc tcgatcaacc gctcgagttg
      781 cttaagttca gcaagtgctg aaggctgata gacaagttca ttggttgtcg gatcgcgata
      841 ctgctcggag cctccaagca agacctgatt caatgaatcc aattgcttct gcaccaactc
      901 gataggattg ttcgctttgt attcccgaat cgcgtttcca gcaccccaag cgtcagtcgc
      961 ggcctgaaca aatgcagaac taacagactc taccgcttcc ttggtgttct cggcagtcac
     1021 aagcctcgcc cgctccaaaa acaagtccgc cagaatcccc gtcttcaact tgacgccctt
     1081 ggccgagttg gctttcgtgg aaatggtcag cacgggagca taggtagtgc cgtaaggagt
     1141 atcttcctcg accaccagtt tgacactgat cgtcttggca aactcttcca acttggtctc
     1201 ctgaagttcc cgctcgtcga agagatcggc tccgatccag tcggttgccg tcaagccttg
     1261 ataggtgcgt tctcccgcag cacttctgcc accggtgaga aacaaggctt catccatgga
     1321 acaaccggta atccgcaaca tttcctcaac tgtaagagag tcggctttct tgaatccttc
     1381 cgcaacgaaa tccctgttct ttttctggtg tgcccagtcg aggtcgggat agctttttct
     1441 gatggcctga atggctcgac tgctgagcct caaacgatca agcaaatcct gcaccagatc
     1501 gggggagttg aacagctccg aataggtttt catggaaaac tgctcgggca gcaaagactg
     1561 ttgttctcga tacttgggag gaacaacgat cagtttggct tccgactggt attcttcgtt
     1621 gataatcagg ctgtagatgg cagtcaatac ggcaacacag aaaggaacga agacaatcca
     1681 gcgccatcgc gcccgaaaaa cgggaatcat cacagttcgg acgaaatccg caggcacacc
     1741 attctttcgg gtttcgacat gtggttcggt catgagtcct ccatcatagg gaagaagcct
     1801 tccgaggtgc cctctcaggc ttcactggaa tacaactgcc ataacggttg caaatgctac
     1861 cacagccgct gccagtctgc aaagaatttg gagatggcag gaagagttca cggcagataa
     1921 cgatgactat ctgctctcca ggaactcctg actcttcagt atcaaaagaa catcacccga
     1981 gtcaaaaaga cctgtttgat cgtagtccaa atcagcgctc tgccaatacg cggaagcctg
     2041 tagccagtca aatgcgtcca cttgcccatc gctgttga
//
LOCUS       DAOQUC010000748         2071 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_68532_length_2071_cov_2.842262, whole genome shotgun sequence.
ACCESSION   DAOQUC010000748 DAOQUC010000000
VERSION     DAOQUC010000748.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68532_length_2071_cov_2.842262"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1987)
                     /locus_tag="PKH07_12210"
     CDS             complement(<1..1987)
                     /locus_tag="PKH07_12210"
                     /inference="COORDINATES: protein motif:HMM:NF012830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35750.1"
                     /translation="MTDESKAFGLITGQPTRSGASNRPQRLGEMEIWGLAAYEADKIL
                     SSFLTIRSDPSPTARCEAYGQPFRVIADHLFALGILLEMDETGRYKYSFITDEMIREK
                     CKGREISSNKTWKRGVRGTFCCRKCSSQVPFEEILATGERQRSRDPYLTVSDLFAHCG
                     YRIHRYGDTEAGDMAIVDEGSPKTARIFLKNGTEETVTEIAAERHRRIVKVSFAFQNS
                     KYTARKQISADKAFFISEIGELEIRCSKHLDKPLTILGCKPDIVPVPGGLADPAFFGE
                     PDPGNAESSRWAFIRLPDGVRVRAGFPGSSMQSWSASYPSQEPLISMIPVLPLRYRFP
                     FPHVSLDGSIHLEETLHGLYQEVLDLLLKEGVAAEEIQSASRCIFAHIGRRLFGKNGL
                     LRKNGLGRRVDGSARMVIAPDPTLNWDECGIPSMVMLSLLLHRLEDSAVPEMVAHARL
                     DGASLPEADDVLCALKMLRLGRVSDFCRVNAGNLDGYHLFAKRLLSQNSHVRLLLNRQ
                     PTLHRYNIQSFKPVLVSPQSGLALRLNPLVCGAFNADFDGDTMSVYLMNSQSESSEAD
                     SLLSPTSGRNLISLANGVSVATFEQDLALGMYLVSQSPDRRQSFLNSGFPFCDECRSL
                     LDAEHWDKRLFDQFLTHTCLSHADVLAQYLSDWSRFAL"
BASE COUNT          522 a          526 c          523 g          500 t
ORIGIN      
        1 ctagagcgaa acggctccaa tcagaaagat attgtgccaa gacgtctgcg tgactcagac
       61 aggtgtgtgt gagaaactgg tcaaacagcc gcttgtccca atgctcagcg tcaagaagac
      121 ttctacactc atcacagaag ggaaagccgg aattcaggaa actctgccgt ctgtcaggac
      181 tttgggacac cagatacatg ccaagagcca agtcctgttc aaatgtggct acggatacac
      241 cgttagccag agagatcagg tttcttcccg aagtgggaga gagaagcgaa tcagcttcag
      301 acgactcact ctgactgttc atcaagtaaa cagacatcgt atctccatca aagtctgcgt
      361 tgaacgcgcc acagacgagt gggttcagtc gcaaggcaag tccactttgt ggtgatacta
      421 ggacgggttt gaaagactga atgttgtacc tgtggagagt tggctgccga ttcagaagga
      481 gacgaacatg ggagttctga gacagaagtc gcttggcgaa caggtggtag ccatcaagat
      541 ttcctgcgtt gactctgcag aagtctgaca cgcgtcccag acgaagcatc ttcagtgcac
      601 agagcacatc gtctgcttcc ggcagactcg caccatcgag tctagcatga gctaccattt
      661 ctggaacggc gctatcctca aggcggtgca agagtagtga caacatgacc atggaaggta
      721 tgccacactc atcccagttc agcgttggat caggagcgat gaccatacgc gctgagccgt
      781 ccactcttct cccaagaccg ttctttcgca agagaccgtt ctttccgaag agacgtcttc
      841 cgatatgtgc aaagatacat ctcgaagcgc tctgaatctc ttcggcggca acgccttcct
      901 taagtaacag atcgagaact tcctggtaca gtccgtgtag cgtctcttcg agatgaatgg
      961 aaccgtccag actgacatgc ggaaagggaa aacggtatcg taggggcaag accgggatca
     1021 tcgagatcaa gggttcttga cttggatatg atgccgacca cgactgcatc gatgaaccag
     1081 ggaatccagc gcgaaccctg actccatcgg gcagcctgat aaacgcccaa cgggaggact
     1141 ctgcatttcc cggatccggt tccccaaaga aagctggatc tgcaagacct cctgggacag
     1201 gaactatgtc tggcttgcat ccgagtatag taagaggctt gtcaagatgc ttggaacagc
     1261 ggatttcaag ttcacctatt tctgagatga agaaagcctt gtctgcgctg atctgcttgc
     1321 gtgcagtgta tttgctgttt tgaaaggcaa aagagacttt gacaatccgc ctgtgtctct
     1381 ccgcagcaat ctcagtcact gtctcttctg ttccattctt gagaaagatc cttgctgttt
     1441 tcgggctgcc ttcatcaaca atggccatgt cgccggcttc agtatcaccg tatctatgaa
     1501 tcctatatcc acaatgcgcg aacaggtcac tgacggtcag gtatgggtct ctggaacgct
     1561 gcctttcccc tgtggcgaga atttcctcaa atggaacttg ggagctacat ttccggcagc
     1621 agaatgtccc tcgaactcca cgtttccatg tcttgtttga agagatctct ctgcccttac
     1681 atttctctct gatcatctcg tctgtgatga aggagtactt gtatctgcca gtctcatcca
     1741 tctcaagaag tatacctaat gcaaaaagat ggtctgctat caccctgaag ggctgtccgt
     1801 aagcctcaca tcgagccgta ggagatggat cggaacgaat tgtcagaaag gatgagagaa
     1861 tcttatctgc ttcatatgcc gcaagacccc agatttccat ttcgcccaga cgctgtggcc
     1921 ggttggatgc tccactccgt gttggctgac cggtaatgag accaaaagcc ttactctcgt
     1981 ctgtcatcgg tcgtacaagg actttcttct ctgggagatg gttcagtctg tggaaatgct
     2041 ggatgcccac cattacggga gcagaaacag t
//
LOCUS       DAOQUC010000749         2070 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_68598_length_2070_cov_2.517618, whole genome shotgun sequence.
ACCESSION   DAOQUC010000749 DAOQUC010000000
VERSION     DAOQUC010000749.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68598_length_2070_cov_2.517618"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..292
                     /locus_tag="PKH07_12215"
     CDS             <1..292
                     /locus_tag="PKH07_12215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014474683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleoid-structuring protein H-NS"
                     /protein_id="HNT35751.1"
                     /translation="ANYLDATIGGLGRGAGNCPLELLLGFLKNPKFKLRPVLKSLQEH
                     FLPLSKKVEWGFTIPYMITGQLNMHPRAAIKCREGDNRDDYVAFYDQLIEQE"
     gene            298..1866
                     /locus_tag="PKH07_12220"
     CDS             298..1866
                     /locus_tag="PKH07_12220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35752.1"
                     /translation="MPGLSEFLLCCATLVMYAGVALADTEAPMESLLLTPTACTDREH
                     LQLRSDGSFALEEGWGSGYCVWDITRERAFQLLDLSVCSEVKHVSGFTPAGDIIVQVR
                     THKEDGTNGRWTEWWPQNLLRGGSFRDATGNGIPDGVDILEFGEQGRLWTDNPREGLS
                     KWFGAVFTESDPPMVSQRDGNSIFRFCKARADGQTIVQMHSDRVPTAQYLTVSGWNGW
                     DLGNAQTMGLMSRFHEIDAQGRRLNKLMFIGDDDWNQPGGISPLMWRAVTFKPRRETR
                     RLNLYAARLISSSGTLECAKYQVHQDSLASEQHLGVCLKRFDRMNSEEWSFSDPDLSF
                     ADEHSLQMRPHPHSNSSALGSCFALCGARRVCVIAEMDVQVPERYDDHDPSHKAWASS
                     YLELLDSEERVAGVVKVSVCRPRFGDELACAGEVPSNAVKARLRLVASHQSYLQPDDR
                     KNMDGEMFCRWSQIRVFESRYPQEFRTRLDEDSFRPGEVPLTRAAEVRAIVLGDSSES
                     SPVLRSIRVELNTR"
BASE COUNT          458 a          513 c          592 g          507 t
ORIGIN      
        1 tgccaactat ctggacgcta ccattggcgg ccttggccga ggggcgggga attgtccgct
       61 ggaactgctc ttgggattcc tcaagaatcc gaaattcaaa cttcgtcccg ttttgaagag
      121 cctgcaggaa cattttctgc cactcagcaa gaaggtcgaa tggggcttta cgatccctta
      181 catgatcaca ggccagctca acatgcatcc acgcgctgct atcaagtgtc gtgaagggga
      241 caaccgtgat gactatgtcg ccttctacga tcagctgatc gagcaagaat agtcgaaatg
      301 ccagggcttt ctgagtttct gctgtgctgt gccacacttg tcatgtatgc cggtgtcgct
      361 cttgcggaca cagaagcacc gatggaatct ctcctgctga ctcccaccgc ctgcaccgat
      421 agggagcatc ttcaactgcg aagcgatggt tcctttgctc tggaggaggg ttggggttcg
      481 gggtactgtg tctgggatat cacacgcgag cgagccttcc aactccttga cctgtccgtc
      541 tgttcagagg tcaagcacgt ttcaggtttc acccctgcgg gagatatcat tgttcaggta
      601 cggactcaca aagaggatgg tactaacgga cgatggactg aatggtggcc tcagaatctc
      661 ttgcgtgggg gaagttttcg ggatgccacg ggtaatggca ttcccgacgg tgttgacatc
      721 cttgaattcg gagaacaagg caggctctgg actgacaacc cgcgggaggg actctcaaaa
      781 tggttcggtg ccgtgttcac agaatcagat cctccaatgg tctctcagag ggatggcaac
      841 agcatcttcc gtttctgcaa agcccgtgca gatggtcaga cgatcgttca gatgcattca
      901 gatcgagtgc caactgccca atatctgact gtgtccggtt ggaatggatg ggatctggga
      961 aatgcacaga cgatggggct gatgtcccgg tttcacgaaa ttgatgctca agggcgtcgt
     1021 ctgaacaaac tcatgttcat cggtgatgac gactggaacc agcctggcgg gatctctcca
     1081 ttgatgtggc gggctgtgac tttcaaaccc cgaagagaga cgcgacgcct caatctttat
     1141 gcggcgcgat tgatctcctc ctctggaacg ctcgaatgtg caaaatacca agttcatcaa
     1201 gacagccttg ccagcgagca acatcttggc gtgtgcctga agaggtttga tcgcatgaac
     1261 tcggaggagt ggtcattttc agatcccgat ctttcgtttg ctgatgagca ctctcttcag
     1321 atgcgacctc atccccacag caactcttct gccttgggtt cgtgttttgc tctctgcggt
     1381 gcgcggagag tttgcgtcat cgccgagatg gatgttcagg taccagagcg atacgatgac
     1441 cacgacccgt cgcacaaagc ctgggcgtca agttatctgg aactcttaga ctcggaggaa
     1501 agggttgcgg gtgtagtgaa ggtctcggtc tgccgaccgc ggttcggtga tgaactggcc
     1561 tgtgccggcg aagtgccatc caatgcggtc aaggcgcggt tgagacttgt ggcatcacac
     1621 cagagttatc ttcagcccga cgacaggaag aacatggatg gtgaaatgtt ctgcagatgg
     1681 agccagattc gggtattcga gagccgctat ccacaagagt tccgcacgcg cctggacgag
     1741 gattcgtttc ggccaggcga agtgccattg acaagggctg cagaggttcg tgcgattgtt
     1801 ctgggtgact cctctgagag ttcacctgtt ctgcgttcga tcagagtcga attgaacaca
     1861 cgataggcga ttttggaacg aaaatcctta ttcggaactc tttctgtttg acagtaaccg
     1921 ctttcagtat acaattacct tgggcagtcc ggtattcatt gggaaggcaa gtccagacgg
     1981 gttcctgttg gttggacaag aagaattgcg ttcatgcagt caggagcagg ctagaatctt
     2041 tgagtgtgcg ggctgtaccc actccatcgg
//
LOCUS       DAOQUC010000750         2069 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_68629_length_2069_cov_4.039722, whole genome shotgun sequence.
ACCESSION   DAOQUC010000750 DAOQUC010000000
VERSION     DAOQUC010000750.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68629_length_2069_cov_4.039722"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /locus_tag="PKH07_12225"
     CDS             complement(<1..407)
                     /locus_tag="PKH07_12225"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNT35753.1"
                     /translation="MKHTGLIFCRETKSYFSTPVAYVVIALFLIMAGITFSLYLTGFV
                     EYCERYVKNAIEDTEDPSAVTPNINELLFGGVLGNMSLFLLFVLPFMTMRLFAEEKRT
                     GTAELLLTYPLTDSEIILGKFSACVFLLAIMLL"
     gene            complement(407..1354)
                     /locus_tag="PKH07_12230"
     CDS             complement(407..1354)
                     /locus_tag="PKH07_12230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNT35754.1"
                     /translation="MIEVVHLTKTYANRTAVNNLCFKIEKGQIVGFLGPNGAGKTTTM
                     RMITGFLSPTEGTCRVAGYDVLSEPMEVRKRVGYMPENVAVYREMSVNAYLTFVGKAK
                     GLSRSEVRGAVEQVIAQCSLDQVASRPTGKLSRGYRQRVGLAQALVASPEVLVLDEPT
                     VGLDPRQIVGIRELIKSLAGKHTIILSTHILPEVSMTCQKVIVINEGRLVEEDTPRNL
                     AERLEKICLVTAEIVGSQEAILEVIRSTSGVVDVRLEKSEHSKHTISIRMNPDDECRT
                     QLCRTIVEKGFGLVEMRRTKITLEEIFIQLVDEDKGSDL"
     gene            1705..>2069
                     /locus_tag="PKH07_12235"
     CDS             1705..>2069
                     /locus_tag="PKH07_12235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35755.1"
                     /translation="MSKRLWFVCLIVLGMTVGLFPAQAVEQLHFGYSASYPGLTDDLR
                     NSTRIGYVFWSLNSTGTGLPYCYHDGYVEWIGVQKTAAQASLRWQQIRASDGIGDTWT
                     GASEATIGTPTIICLDEVNP"
BASE COUNT          486 a          533 c          519 g          531 t
ORIGIN      
        1 atcagcaaca tgatcgccag aagaaagaca cacgcagaga attttccgag gataatctcg
       61 gaatccgtga gtgggtatgt caggagcagt tctgccgttc cggttcgttt ttcttcagca
      121 aaaagccgca tcgtcatgaa agggagcaca aagagaagaa acagggacat gtttccaaga
      181 actcccccaa agagcagttc gttgatgttc ggtgtgactg cactgggatc ttccgtgtcc
      241 tcgattgcat tcttgacata ccgctcgcag tactcaacga agcctgtgag atacaacgag
      301 aaggtgattc cggccataat caggaagagg gcgatgacca catacgcaac gggtgtgctg
      361 aagtaggact tggtctcacg gcagaagatg agtcccgtgt gtttcatcac aagtcgctcc
      421 ctttgtcctc atcaaccagt tgaatgaata tctcttccag cgtgattttg gttcgacgca
      481 tttcgacaag accaaaaccc ttttcgacga ttgttcgaca caactgcgtg cgacactcat
      541 catccggatt catccgaatg cttatcgtgt gcttggaatg ctcagacttt tccaagcgta
      601 catccacaac acctgaagtg ctcctgatga cttcgagaat tgcttcctgg gaacccacga
      661 tctccgccgt caccaggcag attttctcaa gcctttcggc cagatttctc ggcgtatctt
      721 cctccaccag acgcccttca ttgatgacga tgactttctg acaggtcata ctcacttcgg
      781 gcaggatgtg ggtcgagagg atgattgtat gcttgccggc aagtgacttg attagttcgc
      841 gaatcccgac tatctgtctc gggtctaagc cgacagttgg ctcatccaaa acgagtacct
      901 cgggactggc taccagtgct tgtgccaaac caaccctttg gcggtatcct cgcgagagtt
      961 tgcctgtcgg tcgtgaggca acctgatcca ggctgcactg tgcgatgacc tgctctaccg
     1021 ctcccctcac ttctgatcgc gaaagacctt ttgcctttcc gacgaaagtc aggtaggcgt
     1081 tcactgacat ctccctgtag acggctacat tttcgggcat ataaccaacg cgcttccgca
     1141 cttccatcgg ttcgctcaga acatcgtatc cagcaacacg gcaggttcct tctgtggggg
     1201 agaggaagcc cgtgatcatc cgcatcgtgg tcgtctttcc tgcaccattg ggacccagaa
     1261 aaccgacgat ctgtcccttt tcgatcttga agcagagatt attgaccgcc gttcgattgg
     1321 cgtaggtctt ggtcaagtgg acgacttcaa tcatgaggat tcttccttac ttgacacaaa
     1381 cattacaacc atccgttcgt ggaaggcgaa caaaatctgt ttccagccac ctcgaatgga
     1441 catgtgtagc aactctagtg tacttgtggt gttgtcgcaa gcagatttgt gaacagttgc
     1501 gcaaatatcc cttgacacaa ccatgactcg gggaataatc ttcaattcgt cagacgccgt
     1561 tccatagttt gccagaccag gagatcgttg ccggctggaa gattgggagg tggtctcagg
     1621 aatatgcgtt gtggaacctg agatatgggt tccttgtttt ctgggttctc gagttttcgg
     1681 cctaaaaatt aaggagagag caacatgtca aaacgactat ggttcgtttg tttgattgta
     1741 cttggcatga cagtggggtt atttccagct caggcggttg agcaactgca ttttggctat
     1801 agtgcgagtt atcctggtct gactgacgat ctgagaaatt caacacgaat cggctatgtc
     1861 ttctggtctc tcaactccac aggcaccggc cttccttact gctaccacga tggctacgtg
     1921 gagtggatcg gagttcagaa gaccgccgct caggcgtcgc tgcgttggca gcagatcagg
     1981 gcaagtgacg gaattgggga cacatggact ggagcttctg aggcgaccat tggcacccca
     2041 accatcatct gccttgatga agtcaaccc
//
LOCUS       DAOQUC010000751         2068 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_68693_length_2068_cov_3.284650, whole genome shotgun sequence.
ACCESSION   DAOQUC010000751 DAOQUC010000000
VERSION     DAOQUC010000751.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2068
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68693_length_2068_cov_3.284650"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            5..1141
                     /locus_tag="PKH07_12240"
     CDS             5..1141
                     /locus_tag="PKH07_12240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015722819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNT35756.1"
                     /translation="MQRMSLVLLVLMVTHVGWCADTIKVGALFSVTGKGSFLGEPEKN
                     TAAMIAEEINAKGGINGKKIELLVEDTESSEATAVMAAQKLISRDQVVAIIGPSTSGE
                     TMAVLRICEEQKVPLVSCAAAAPIVQPVAERKWIFKSPQMDSDCVVRIFEHMKTKGID
                     KVGIITITDGFGKSGRDQLLKLATEMEITVVADETYAPTDTDMTAQLTKIKASEAKAV
                     VNWSIGPTQSLIPKNMKQLGMTIPLYQSHGFGNLKYVEAAGDSAEGIIFPAGRLLVAD
                     ELPADHPQKKVLVDYKTAYEAKFGGSVSTFGGHAYDALHLVAKAAVLGAPTREGIRDG
                     LEKIQGFVGTAGIFNMSDTDHLGLTKDAFEMLTVKNGKFVILKD"
     gene            1241..>2068
                     /locus_tag="PKH07_12245"
     CDS             1241..>2068
                     /locus_tag="PKH07_12245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015720373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNT35757.1"
                     /translation="MSLYSQILQFILTGLTVGSIYGLVAVGFNIIYNLTGIINLAQGE
                     FVMLGGMSAVWLVESLHVPVFLAFPISVLMVTIVGILMETLAIRPVKKADVLSMIICT
                     VGASVFLKGLVMFVWGKQTHALPHFSTENAIFIAGASILPQTLWVWGITALIVFGLWL
                     FFDKTLLGKAMRACSYNRDAASLVGISATKMVMLSFALSAAVGAAAGVAITPMTLMSY
                     ERGTMLGLKGFAAGILGGLGNPFGGVVAGLMIGVLEGLAAGSRLSHYKDAVALVVLLA
                     "
BASE COUNT          469 a          482 c          594 g          523 t
ORIGIN      
        1 gaccatgcaa aggatgtcat tggttttact tgttcttatg gtaacgcatg tgggttggtg
       61 tgctgataca ataaaggtcg gcgcactctt ttctgtcacc ggcaaaggat cttttttggg
      121 tgagccagag aagaacacgg ccgccatgat cgccgaagag atcaatgcca aagggggcat
      181 caacggaaaa aagatcgaac tgcttgtcga ggacacagag tccagtgaag cgacagccgt
      241 tatggcagca cagaaactga tctcaagaga tcaggtcgtg gcgatcattg gaccgtccac
      301 cagtggagag acgatggctg tgttgagaat ctgcgaagaa cagaaagttc ccttggtttc
      361 ttgtgctgca gccgccccta tcgttcagcc ggtcgccgaa agaaagtgga ttttcaagag
      421 tccgcagatg gactccgatt gcgtcgttcg aatcttcgag cacatgaaga ccaagggtat
      481 tgacaaggtc ggtattatca ccatcaccga tgggtttggg aaatctggcc gtgatcagtt
      541 gctgaaactt gccacagaga tggagatcac ggtggttgcg gatgaaacct acgctcccac
      601 ggacacggat atgaccgcac agttgaccaa aattaaggca tcggaagcca aggccgttgt
      661 gaactggtct atcgggccaa ctcagtcgct tatccccaag aacatgaagc agctgggaat
      721 gaccattccc ctctatcaga gccatggttt tgggaacctg aagtatgttg aggcggctgg
      781 tgattcagcg gaagggatca tcttcccagc gggacgtctt ctggttgctg atgaactgcc
      841 tgcagatcac ccgcagaaga aggttcttgt tgattacaag acagcctatg aagcgaagtt
      901 cggtggttct gtgagcacat tcggtggtca cgcctatgac gcattgcatc tcgttgcgaa
      961 agccgctgta ctcggtgcac cgacacggga aggcattcga gacggtctgg agaagattca
     1021 aggttttgtt ggcacggcag gtatattcaa catgtccgac accgaccacc ttggtctcac
     1081 caaagacgca tttgagatgc tgaccgtgaa gaacggcaag tttgtcattc ttaaggactg
     1141 agctgtctca ttgtggaacg ttccgcaatg gtgacgcaga aatactgaat cgagtcctcg
     1201 cccgttccca aggggacggg cggggacagg ttttgaaggt atgtcgcttt acagccagat
     1261 tctccaattc atcctcaccg gcctgactgt tgggagtatc tacggcctag tggccgtagg
     1321 gttcaacatc atctacaact tgaccgggat tatcaatctt gcccagggag agtttgtgat
     1381 gttgggtggg atgagcgcgg tctggctggt tgaaagtctt catgttcctg tctttctggc
     1441 ctttcccatc agcgttctga tggtgacgat tgtcggaatc ttgatggaga ctcttgcgat
     1501 tcgaccagtc aagaaagctg acgttctttc gatgattatc tgcactgtgg gcgcgtccgt
     1561 cttcttgaaa ggacttgtca tgttcgtctg gggtaagcag acgcatgcgc ttccccattt
     1621 ctcaacggaa aacgctatct ttatcgcggg cgcttcaatt ctgcctcaga cgctgtgggt
     1681 ttggggtatc acagcactga ttgtattcgg actttggctc ttttttgaca agacgctgct
     1741 gggcaaagcg atgcgtgcct gctcctacaa tcgggatgcg gcatcattgg tcggcatcag
     1801 cgccaccaag atggtcatgt tgtcttttgc ccttagcgcg gcagtcggag ctgcagcggg
     1861 tgtggcaatc acacccatga cattgatgag ctatgaacgg ggcactatgt tgggactaaa
     1921 gggcttcgcc gcggggatac tggggggctt aggaaatcct tttggcggag ttgtcgcagg
     1981 ccttatgatt ggggtattgg aaggtctggc ggcaggttct cgactctctc actacaaaga
     2041 tgctgttgct cttgtggtac tcctggca
//
LOCUS       DAOQUC010000752         2067 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_68747_length_2067_cov_3.405070, whole genome shotgun sequence.
ACCESSION   DAOQUC010000752 DAOQUC010000000
VERSION     DAOQUC010000752.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2067
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68747_length_2067_cov_3.405070"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1799
                     /locus_tag="PKH07_12250"
     CDS             <1..1799
                     /locus_tag="PKH07_12250"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNT35758.1"
                     /translation="AYGIVEKATAEDPRLPGAWALPETCFRKASVLYEQKRFQDAAAT
                     LKKGIADHSKSDLLLEMQHSLGWAYFGMKDYTRAIELADSLIGSPTASKRLQGLSLLR
                     ANAHYELTNYNEAITSYSEVVRLVSDDDESRKQGAEALARIAWCHYLAGRHDEALEAV
                     DVALKAAENAAFVGDLKFLRGQILYLKNDPAKALLEFLDVLANHKESRFHSEVGYRLG
                     WSYFKLEQYQEAAEVWFTYALKYPDQDTGREAALMAGEATFKAGDFEQSLELFDHYIT
                     TYPQMPESESVLYRASVACYSLQKYDKMSAYLTQLIERFPQTTYLAEAYYWLGFAASV
                     REQTAESERYFSLLVKTQPTSEYASDALYRLGKIYNQTKRAQESCEAFLRIVKEYPNH
                     VLPAEMLFWLGAQLWDAKRYDDAFRSYASLWEKHPSSELKGRAQFEAGRCLMQAQKLA
                     EAEALYRRMLEENQHGGYRGLASYGLAIVLMDKKDYSGALQQLLDCRDSLPDEWAADA
                     GFRIGVCHRELGQATEAFEAFMRVAILYDLPTLSAQALWEAGQASDRLSDATESEKTY
                     RELIKRYGETEWGNKARELLNARASQPVSATE"
     gene            1796..>2067
                     /locus_tag="PKH07_12255"
     CDS             1796..>2067
                     /locus_tag="PKH07_12255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35759.1"
                     /translation="MMKTATQAHSPSLMSGREFKIALALALLVHALILGLSPFIKFWG
                     MGQVEHEAYRLFRVNVVNIDPNRFSRVFASQDALRETLQKEAEQIA"
BASE COUNT          495 a          545 c          583 g          444 t
ORIGIN      
        1 aggcatacgg catcgtggag aaagccacgg cggaggatcc ccgactgcca ggagcgtggg
       61 ctctgccaga gacctgtttt cgaaaggcat cggtgctcta cgaacagaag cgattccagg
      121 acgcggcggc aacactcaaa aaggggatcg cagaccattc aaaatcggac ttgctgctgg
      181 agatgcagca cagtctgggc tgggcttact ttggcatgaa ggactacacc cgcgccattg
      241 agttggcgga ttcactcatc ggatcaccca ccgcgtccaa gcgtcttcag ggtctgtctc
      301 ttctgcgcgc caacgcacac tatgaactga ccaactacaa cgaagcgatc acctcttatt
      361 cagaagtggt gcgacttgtt tctgacgacg acgagtcccg caaacagggc gcagaagctc
      421 tggccagaat tgcatggtgc cattatcttg cggggcgtca tgacgaagcg ctcgaagccg
      481 ttgacgtggc actgaaggca gccgaaaacg ctgcatttgt gggtgacttg aagtttctca
      541 ggggacaaat cctctacttg aagaatgatc ccgccaaagc gcttctggag tttctcgacg
      601 tgcttgccaa tcacaaggaa tcgcggttcc attcagaagt cggatatcgc ctgggctggt
      661 cttacttcaa actcgaacaa taccaggagg ccgcggaagt ctggttcacg tatgccttga
      721 agtatcccga tcaagacaca ggtcgcgaag cagcactcat ggctggcgag gcaaccttca
      781 aggcgggtga ctttgagcaa tcgctggagt tgttcgatca ttacatcacg acctatcccc
      841 agatgcccga gagtgagtcg gttctctatc gggcttcggt ggcctgttac agtcttcaga
      901 aatacgacaa gatgagtgcc tatctcacgc aactgattga acgcttcccc caaaccactt
      961 atctggctga ggcgtattac tggcttggat ttgccgcttc tgtccgcgaa cagaccgcgg
     1021 agtccgagag gtacttctca ctgctggtca agacacagcc aacgtccgaa tacgcaagtg
     1081 acgcgctgta ccgtctggga aagatctaca accagaccaa gcgagcgcag gagtcctgcg
     1141 aggcgtttct gcgaatcgtc aaagagtatc caaatcacgt tctgcctgcg gagatgctct
     1201 tctggctcgg tgcgcaactg tgggacgcca agaggtacga cgacgcgttt cgcagttatg
     1261 cttcgctctg ggaaaagcat ccgtcgtcag aactcaaggg acgtgcccag ttcgaggcag
     1321 gacgttgcct tatgcaggcg cagaaactcg cggaagccga agccctgtat cggcgaatgc
     1381 ttgaggagaa tcagcacggg ggctatcggg gacttgctag ctatggtttg gcaatcgtct
     1441 tgatggataa aaaggattat tcaggagcgc ttcaacagtt gctcgactgt cgggactcat
     1501 tgcctgatga atgggcagcg gatgccggat ttcggattgg tgtctgtcat cgggaattgg
     1561 gtcaagccac tgaagctttt gaagccttca tgcgggtggc cattctctat gatctgccca
     1621 cactcagcgc tcaagcattg tgggaagcag gccaagcctc cgatcggcta agtgacgcaa
     1681 ccgagtcgga gaagacttat cgggaactga tcaagcgcta cggcgaaacc gaatggggca
     1741 acaaagcccg agaactactg aatgcacggg catcacagcc cgtcagcgca accgaatgat
     1801 gaaaacagcg acgcaggcac attcaccatc tctgatgtca ggacgagagt tcaagatcgc
     1861 cctggctctc gcgctcctgg ttcatgctct cattcttggc ctgtcgccat tcatcaagtt
     1921 ttggggaatg ggtcaagtcg aacacgaagc gtatcggttg ttccgtgtca atgtggtgaa
     1981 cattgatcca aaccgattca gccgtgtatt tgccagtcag gacgcgttgc gcgagacgct
     2041 gcaaaaggaa gcagagcaga ttgccca
//
LOCUS       DAOQUC010000753         2067 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_68760_length_2067_cov_2.891153, whole genome shotgun sequence.
ACCESSION   DAOQUC010000753 DAOQUC010000000
VERSION     DAOQUC010000753.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2067
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68760_length_2067_cov_2.891153"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            307..1638
                     /locus_tag="PKH07_12260"
     CDS             307..1638
                     /locus_tag="PKH07_12260"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013257968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HNT35760.1"
                     /translation="MSSQDKAKVMELVKEKNVRFIRLWFTDVLGALKSFAITVDELEG
                     ALDEGMGFDGSSIEGFARIQESDMVAMPDPKTFTILPWRPTEEAAVARMFCDILQPDG
                     SPYVGDPRYALRRNLKKAADMGYTYYVGPELEYFYFKNSSGTETLDSGGYFDLTPLDV
                     ASDLRRETVLALESMGIKVEYSHHEVAPSQHEIDLRYKDALTMADAAMTYRLTVKEIA
                     MKNGVYATFMPKPIFGQNGSGMHTHQSLFLGEKNAFFDPKDKHHLSKTGKSYIAGLLV
                     HAREICSVVAQWVNSYKRLVPGYEAPVYVSWAQRNRSALVRVPMYKPGREKATRVEFR
                     CPDPACNPYLAFSVMLAAGLKGVAENYELPAPVEEDIFEMDEQARAKHGIASLPGSLA
                     EAVAETEKSDLVREALGDHIFEKFIANKKIEWDRYRTHVTQLELDKYLPIL"
     gene            1783..>2067
                     /locus_tag="PKH07_12265"
     CDS             1783..>2067
                     /locus_tag="PKH07_12265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005983579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35761.1"
                     /translation="MKRQRDEMKDISGCAISGIMSERRERFSGDMIIRSIATMHERSN
                     GLGGGFAAYGIYPDLQHHYAIHVMCDSQAAKEHAEELLKSRCRIYHEEPIP"
BASE COUNT          508 a          504 c          575 g          480 t
ORIGIN      
        1 gggcacagtg tcggttgggg tcttctcttt cttgacacct acccccaagt tgatcgttca
       61 gtagaatacc tacatggatg taggaatctg tgctattgga cgatctcgaa ctatgcatat
      121 aacatattga taaatagtga gatgattata tttccatatc ttggcacaac tcctgctcat
      181 agacaaatgc gtacgtttat gaaggctctc ggtctctggc ttccatggca cagttgacgt
      241 ttgcgagaga cgatgagcct gtcaggacga gacaagaagt tcactagtct tattgaggag
      301 gagaagatga gcagtcagga caaagccaag gtcatggaac tggtgaagga aaagaacgtc
      361 aggttcatcc gactctggtt cacggacgtg ttgggagcct tgaagagttt cgccatcacc
      421 gtggatgaac tggaaggcgc tctggatgaa gggatgggtt tcgatggatc ttccatcgaa
      481 ggcttcgcgc gaattcagga gagtgacatg gtcgccatgc cggatccgaa gacgtttacc
      541 atccttcctt ggagaccaac ggaagaggct gcagttgccc gaatgttctg tgacatcctt
      601 cagccggacg gttcacccta cgtcggtgat ccgcgctacg cccttcgccg gaatctgaag
      661 aaggcggcgg acatgggcta tacctactat gttggccccg aactggagta tttctacttc
      721 aaaaacagtt ccggaacgga gaccttggat tcgggtggct acttcgatct gactccattg
      781 gatgttgcca gtgatctcag gcgtgagacc gtacttgcct tggaatccat ggggatcaaa
      841 gttgagtaca gccatcacga agtggctccc agtcagcatg aaattgacct tcgttacaag
      901 gatgccttga cgatggcgga cgccgcaatg acctatcggc tcacagtcaa ggagatcgcc
      961 atgaagaatg gtgtctacgc cacattcatg ccgaaaccca tctttggaca gaatggcagc
     1021 gggatgcaca cgcatcagtc cttgttcctg ggtgagaaga atgccttctt cgatcccaag
     1081 gacaaacatc atctttccaa gactggcaag tcgtacatcg ccggattgtt ggttcatgcg
     1141 cgcgaaatct gctcggttgt ggcacaatgg gtgaattcct acaagcgact cgttccgggt
     1201 tacgaagcgc ccgtctatgt ctcctgggcg cagcgcaacc gttctgctct ggttcgcgtt
     1261 ccgatgtaca agccggggcg agaaaaggcg acccgtgttg agttccggtg tcccgatccg
     1321 gcgtgcaatc cgtatcttgc cttctccgtc atgttggcag cgggactgaa aggcgttgct
     1381 gagaactacg aactgcctgc acctgtcgaa gaagacatct tcgagatgga tgagcaggca
     1441 cgcgccaagc atggcattgc ctctcttcct gggagtttgg ccgaagcagt ggcggagact
     1501 gagaagagcg acttggttcg tgaagcactt ggcgatcata tttttgaaaa attcatcgcc
     1561 aacaagaaga tcgaatggga tcgctacagg actcatgtga ctcagcttga actggataag
     1621 tatctgccca ttctgtaaaa cggaacatgc aggtaccggc aggcgtgaga caataatcac
     1681 tcctgccggt gctcgcttct tttgggagtt tcggcaccca gttctgtttg caggatagag
     1741 tcagggtaag aagatccgat ccaagctgtg acagggaata gaatgaagag acagcgagac
     1801 gaaatgaaag acatcagcgg ctgcgccatc tccgggatca tgtccgaacg gagagagcgc
     1861 tttagcgggg acatgatcat ccgttcgatt gccaccatgc acgagcgatc caacggactg
     1921 ggtggtggat ttgcagcata cggaatttac cctgatctcc agcatcacta cgcgatccat
     1981 gtcatgtgtg acagccaggc ggccaaagag cacgcggaag aacttttgaa aagccgctgt
     2041 cgaatctacc atgaagagcc gatcccg
//
LOCUS       DAOQUC010000754         2065 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_68853_length_2065_cov_3.679602, whole genome shotgun sequence.
ACCESSION   DAOQUC010000754 DAOQUC010000000
VERSION     DAOQUC010000754.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68853_length_2065_cov_3.679602"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="PKH07_12270"
     CDS             <1..318
                     /locus_tag="PKH07_12270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35762.1"
                     /translation="TTVELPGKLSRAVCQTSTSTWPHTFVVPKYATMIEYKQFAPANH
                     FHMTWGLEPSRVQYWMDLANVLSVTPWQARPGFIENVDRPTPLLFLLNGGENQAKMLR
                     MKR"
     gene            421..1878
                     /locus_tag="PKH07_12275"
     CDS             421..1878
                     /locus_tag="PKH07_12275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNT35763.1"
                     /translation="MSKIHRRDFLKSSIGATAALFAGCATAPHARVLGANDDVRVGVI
                     GLRNKGQHLVRDFLAVKGARVVAVCDADTAFLDREVEAAGKRKQTLTVYQDLRRMLES
                     PDIDAVVIAMPNFWHSLATVWACQAGKDVYVEKPVSHNIWEGRKMVEAARKYKRVVQA
                     GTQKRSDPGAQEAFAYIQQGHLGRIKVVRGFCFRERGSIGIADGTQQVPATVNYDLWC
                     GPTPLAPLNRKSLHYDWHWFWSTGNGEIGNQGIHEMDMCRWALGQNTLPPRVLSLGGR
                     FGYEDDAETPNTQVAILDYEPAPIIFEVRGLYRKASDKAMDHYRNIRVGLVVECEDGY
                     YAGGGGGWIFDHDGNRIKQFQGDGGGQHATNFIKAVRSRKTEDQVADILEGHLSSALC
                     HMANISQQLGSRASSDEIQEKIRSDRHALETLERFQSHLEANEIDLAKTPVVLGPWLA
                     MDAEKERFVNCPDADAANKLVSRKYRKPYIIPERV"
BASE COUNT          456 a          531 c          615 g          463 t
ORIGIN      
        1 actacggtgg aactccctgg caagttgtcc cgcgcggtct gccagacttc gacctccacg
       61 tggcctcaca catttgtggt gcccaagtac gcgactatga tcgagtacaa gcagttcgca
      121 ccggcgaacc attttcacat gacctggggc ttggagccgt cgcgtgttca gtactggatg
      181 gacttggcaa acgttctctc ggtcacaccg tggcaggcgc gtcccggctt catcgaaaac
      241 gtggacagac cgacgcctct tctgttcctt ttgaacggtg gagagaatca ggccaagatg
      301 ctgcgtatga agagatgact tcccctgagg gggcgtctgt gctcatgtgt tgttgcctca
      361 gagagatgaa atacgtcttg ctgaactgag gccatctcag caatcgaaag gatcgagttg
      421 atgagcaaga ttcatcgaag ggactttctg aaaagcagta tcggagcaac cgcggcgctg
      481 tttgcggggt gcgcgactgc gcctcatgca agagttcttg gcgccaacga tgacgttcgt
      541 gttggtgtga tcggtcttcg taacaaggga cagcacctgg ttcgagactt tcttgctgtg
      601 aagggcgctc gagtcgttgc cgtttgtgat gccgatacgg cctttctcga cagggaagtc
      661 gaggcagccg gcaagcgcaa gcaaaccctt accgtgtatc aggatctgcg tcgtatgttg
      721 gagagtcctg atatagatgc ggtggttatc gcgatgccga acttctggca ttcgcttgcc
      781 acggtctggg cgtgtcaggc aggcaaagac gtctacgtgg aaaagccggt gtctcacaac
      841 atctgggaag gtagaaaaat ggttgaggcg gcgcgcaagt acaagcgtgt cgttcaggcg
      901 ggaacccaga agcgttctga tccaggtgcc caggaagcgt ttgcctacat tcagcaagga
      961 catctgggca gaatcaaggt ggtgcgtggg ttctgtttca gggagcgagg aagcatcggc
     1021 attgcggacg gcacccagca ggtccccgcg accgtgaatt atgatctgtg gtgtggaccg
     1081 actcctctgg ctccgctgaa ccgaaagagt ctgcattatg actggcactg gttctggtct
     1141 accggcaacg gcgagatcgg caaccaggga atccatgaga tggacatgtg tcgctgggcg
     1201 ttgggtcaga atacacttcc cccgcgcgtg ttgagtcttg ggggtcgctt tggctatgag
     1261 gacgacgcgg agactcccaa cacacaggtg gcaattctcg actatgaacc agctccgatc
     1321 attttcgaag ttcggggtct gtatcgcaaa gcgagtgaca aggccatgga tcactatcgg
     1381 aacatccgtg tcggtcttgt tgtcgaatgc gaagatgggt actacgcggg cggcggtgga
     1441 ggttggatct tcgaccacga cggcaaccgt atcaagcagt ttcagggcga cggtggcggg
     1501 cagcacgcca ccaacttcat caaggcagta cgcagtcgca agaccgaaga ccaggttgcg
     1561 gatattctcg aaggtcatct ctccagtgcg ctgtgtcaca tggcaaacat ctcacaacag
     1621 ctcggttccc gcgcttcttc ggatgagatt caggaaaaga tccgctccga tcgccacgcc
     1681 ttggagacct tggagcgttt ccagagccat cttgaggcaa atgagatcga tcttgcaaag
     1741 acccctgtag tcttgggtcc gtggcttgcc atggatgccg agaaggaacg ctttgtcaat
     1801 tgtcctgacg ccgacgctgc caacaaactt gtttccagga aatatcgcaa gccctatatc
     1861 attccggaaa gagtctgatt ccgtgcagca ggaaacgccc gacagtatga aacgtacctt
     1921 tcgtagggtc tgtcgcgaac aaagtcgtac gcgatatcct gttgttgtct cagcattgag
     1981 ttcccgaatc gccaagtccg tcctgagcat gaaggagttt catgacgcgg agattctggc
     2041 cgcctatgcc gcgctacccg aggaa
//
LOCUS       DAOQUC010000755         2065 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_68879_length_2065_cov_2.533333, whole genome shotgun sequence.
ACCESSION   DAOQUC010000755 DAOQUC010000000
VERSION     DAOQUC010000755.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68879_length_2065_cov_2.533333"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2065)
                     /locus_tag="PKH07_12280"
     CDS             complement(<1..>2065)
                     /locus_tag="PKH07_12280"
                     /inference="COORDINATES: protein motif:HMM:NF018449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HNT35764.1"
                     /translation="SDGRVVVVGWSNSQDYPLAGTWARPRSSETLSEGVVSVFDSEGR
                     MQWSSYLGGLMDDQCQSVGMDEDFSIYVCGWTSSQDFPLQGFYARNPGGTYGQDAFVT
                     KFLWDGMLDWSTYLGGYNADQGHSVTVAPDGMCYVAGSTLSDDFPVTSDSPYPFGSSY
                     WAGFLTRYSPEGAIVWSTCLAGSLSDECYAVAMDNGRNAYVTGRTDSLDFPLLAPFSS
                     TALPRDTFVSKYALEGHRVWSTYLGVLGNDEGRGIVVDQNGDVVVTGISAPHFPVPPA
                     NLALTEDTTPYNCFATKISQKHDFYVHAEKGNDLSGDGSQTNPWQSITTGLKAAPAGT
                     SDALTNIHVSSAIYSENAVFQNQYTKVFGGYNPEKWDDRDLKAYPTTVDGRMAGPCFA
                     GARGIDIRLDGLTIRNGFSDQVSGGGIQGWTNVLLDDCIVTGCMVAIGDGGGVYGTRV
                     EARRCTFSSNSANSGGGIYGYQVLAENCFFNGNSASSPYAKGGAVWGEYAEVRGCSFQ
                     YNTISGDGECRGGGLAGSLLDISDSHFEGNNSGYYGGGIAVCPTEGNMRGLAMTNTHI
                     LNNKSTSRGGGIHSVYVLSVTDSVIQGNESDVGAGVCVDGLTVGSVFRSRILSNVATG
                     AGGGVSGSIVKLRECLLSGNAASENGGAISVFFPSPGAAGFEPVVSECIIRDNSAISG
                     GGLMQS"
BASE COUNT          493 a          616 c          504 g          452 t
ORIGIN      
        1 ggactgcatc agaccacccc cactgattgc actgttatca cgaatgatgc actccgaaac
       61 cacgggttcg aaaccggctg ctcccggaga tgggaaaaag acagagattg ccccaccgtt
      121 ttcactggcg gcatttccgc tgagaaggca ttccctcaac ttgacaatgc tacctgaaac
      181 accgcctccc gcaccagttg ccacattgct cagaatacgg ctccgaaaaa cggaaccgac
      241 cgtgagccca tccacacaga ctcctgcgcc aacatccgat tcgtttcctt ggataaccga
      301 gtcggtgact gacagcacat agactgagtg aatcccccca cctcgcgacg tggatttgtt
      361 attgagaatg tgagtgttgg tcatggccaa cccacgcatg ttgccttctg tcggacagac
      421 cgcgatgcct cccccatagt agccactatt gttcccttca aaatgtgaat cggagatgtc
      481 aagcagagaa ccggcgagac cgccaccgcg acactctccg tcaccggaaa tggtgttgta
      541 ctgaaaactg cagccacgaa cttcggcata ttctccccac actgcccccc ccttggcgta
      601 tggtgaggat gcggagtttc cgttgaaaaa gcagttttcc gcaagaacct ggtacccgta
      661 tatccctccc ccggaattgg ctgaattgct gctgaatgtg caccggcgtg cctccactct
      721 tgtgccgtag actcctccgc catcgccgat cgccaccatg caccctgtca caatgcagtc
      781 gtcgagcaat acgtttgtcc agccctgtat tccaccgcct gatacctgat cggaaaaccc
      841 atttcttatc gtcaggccat ccagtcgtat atcaatgcct ctggcacctg caaaacaggg
      901 tcctgccata cgaccatcca ctgtggtcgg ataggctttc aagtccctgt cgtcccactt
      961 ctcaggattg tatcctccaa aaaccttggt gtattgattc tgaaagacgg cattctcgct
     1021 gtagattgcg gaagacacat ggatattcgt caatgcatcc gacgtgcctg cgggagccgc
     1081 cttcagccct gtcgtgatgc tctgccaggg attggtctgc gaaccgtcac cgctcagatc
     1141 attccccttc tcagcatgga cgtagaagtc gtgcttctgc gaaatcttag ttgcgaagca
     1201 gttatagggt gttgtgtcct cagtcaacgc gaggttggct ggtggcacag ggaagtgagg
     1261 ggcactgatc cccgtgacga caacgtcacc gttctgatcc accacaatgc ctcgcccttc
     1321 atcattgccc aatactccaa gataggtaga ccatacccga tggccttcga gggcatattt
     1381 actgacgaaa gtgtcacggg gaagagcggt cgaggaaaag ggcgccagga gcgggaaatc
     1441 aagcgaatcc gttcgaccgg tcacataggc gtttctacca ttgtccattg ccactgcata
     1501 gcattcatca cttagcgatc cagccaggca cgttgaccag acgatggcgc cttctggcga
     1561 ataacgagtc agaaaccccg cccagtagga ggaaccgaat ggatagggag agtcactggt
     1621 tacagggaag tcgtcagata aagtcgaacc tgccacatag cacatgccgt ctggtgcaac
     1681 ggtgacgctg tggccttgat ctgcgttata gcctccgagg tatgtagacc agtcaagcat
     1741 cccatcccag agaaacttgg tcacaaaagc atcctgtcca taggtacccc ctggattgcg
     1801 agcgtagaag ccctgcaacg gaaagtcctg tgatgaagtc catccgcaga catagatcga
     1861 gaaatcctcg tccattccaa ctgattggca ctgatcatcc atcagtcctc cgaggtagga
     1921 tgaccactgc attcttcctt cactgtcaaa gaccgagaca actccctcag agagcgtctc
     1981 tgacgaacgt ggacgtgccc aggttcccgc aagcgggtag tcctgggagt tgctccatcc
     2041 gacaacaacc acccttccgt cagag
//
LOCUS       DAOQUC010000756         2064 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_68908_length_2064_cov_3.261822, whole genome shotgun sequence.
ACCESSION   DAOQUC010000756 DAOQUC010000000
VERSION     DAOQUC010000756.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2064
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68908_length_2064_cov_3.261822"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..707)
                     /locus_tag="PKH07_12285"
     CDS             complement(<1..707)
                     /locus_tag="PKH07_12285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35765.1"
                     /translation="MKQIIFSSPHIKRYETDEFRPSTEPRFVAVSLRGSECHLQCDHC
                     RTRMLKALYQVKTPEQLETLTARLVSRGCKGLLVTGGCDSDGTIPLGAFAEQICEIKH
                     RYGLRIATHTKMVTESFASAAVRADVDLVMCDLVGADAPLRSVYHLVDHSAEDVEKSL
                     DISLKHGLKLAPHILIGIDYGKVESEYRALEMLKHRDFEVLALVVLTPLRHTPMAECS
                     IDRDGVLRFLEHAREQFP"
     gene            complement(704..1765)
                     /locus_tag="PKH07_12290"
     CDS             complement(704..1765)
                     /locus_tag="PKH07_12290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006964232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNT35766.1"
                     /translation="MNSDKNNRETHLERESPEYVKLSLAAAMQLGYSPARFHRAAQMT
                     CINLLLTYSAGCAGNCSYCGLARERLSDGDPSFIRVPWPIVEFTNAVGRIAANRVVKR
                     VCISMVTHARSVQDSITMARSLRGNTDKPVSALIAPTLVNEKDLAQLKDCGVDKIGIA
                     FDLPTESLFETHRGSGVRGPHRWKKYWQLFESAVALFGEGNVGSHFIVGLGETEREMV
                     EAFQRVRDLGGVNHLFSFYPEPGSTLTEKTPPAIQIYRRMQIACELIDADRSRANCFR
                     FDDQDRVVDFGVSADELETLIQSGVPFRTRGCVGHDGAVACNRPYANSSPAEGIRNYP
                     FQPNEEDLLLIRKELSGAA"
     gene            complement(1773..>2064)
                     /locus_tag="PKH07_12295"
     CDS             complement(1773..>2064)
                     /locus_tag="PKH07_12295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35767.1"
                     /translation="LLLNENLGVEFANRFVCDLCGNSPREITPEIMLPLGIEAIEHKE
                     LMDTIRSVFSSRIGKLTLIPISAGKQIILAQMSLVMNKEQVRRVLMMIGSGR"
BASE COUNT          503 a          575 c          524 g          462 t
ORIGIN      
        1 ggaaattgct ctcgggcatg ttccagaaac cgcaacacac catctctgtc tatcgagcat
       61 tctgccatcg gcgtatgacg aagcggagtg aggacaacca aggcaagtac ttcaaaatcc
      121 cgatgcttga gcatctcaag tgcacggtat tcgctctcaa ccttcccgta gtctatcccg
      181 atgagaatgt gtggtgccag tttcaaccca tgtttcaggg aaatgtccag agacttttcc
      241 acatcctccg ccgagtgatc caccagatgg tagacactcc gaagaggtgc gtccgcacca
      301 acaagatcgc acatcacaag atcaacatcc gcacgcaccg cggctgaggc gaaggactcg
      361 gtgaccatct tcgtatgggt ggcgatacga agtccatacc gatgcttgat ctcacaaatc
      421 tgttcagcga aagcgcccaa tggaatggtg ccgtccgagt cgcaccctcc cgttacgaga
      481 agccccttgc agcctctcga aacaaggcgt gctgtgagtg tctccaactg ttccggtgtc
      541 ttcacctggt agagtgcctt gagcatgcga gtccgacaat gatcacactg cagatggcat
      601 tcactgccac gaagggaaac ggcaacaaag cgcggttcag tactcggccg aaactcatcg
      661 gtctcatagc gtttgatatg ggggctggag aagataatct gtttcatgct gcgccagaga
      721 gttccttccg aataagaagc agatcctcct cgttcggttg gaatggatag tttcgaatgc
      781 cctcggctgg cgaggaattt gcatagggtc ggttgcaggc aaccgcgcca tcgtgaccaa
      841 cacagcctcg cgtccggaag gggacaccgg actgaataag tgtttccaat tcatccgcac
      901 tgacaccgaa atcaacaact ctgtcctggt catcaaatcg gaagcaattc gcgcgagaac
      961 ggtctgcatc aatgagctcg caggcgatct gcatacgacg gtagatctgt atcgcagggg
     1021 gagtcttttc ggtcagagtg ctgcccggtt cagggtagaa ggagaacaga tggttcaccc
     1081 cgccaagatc gcgaacacgc tgaaaagctt caaccatctc ccgctccgtt tcgccaagac
     1141 cgacgataaa atgacttcca acgtttccct caccgaagag agccactgcc gattcgaaca
     1201 actgccagta cttcttccag cgatgaggac ctcgaacacc agagcctcgg tgagtttcaa
     1261 acagggactc agtgggcaga tcaaaagcga tgcctatctt gtccactccg cagtccttca
     1321 gctgagccag atctttctcg ttcacaaggg ttggtgcaat caaggcggac acgggcttgt
     1381 cggtatttcc tctcagactc cgagccatcg tgatggagtc ttgcacactt cgggcatgtg
     1441 tgaccatcga gatgcacacg cgtttgacta cgcgatttgc ggcaattctg cccaccgcat
     1501 tggtgaactc gacaataggc catggaacgc gaatgaaact gggatctccg tcggagagcc
     1561 gttcgcgcgc aagaccgcag taagagcaat tgcctgcaca accggcagaa tacgtcagca
     1621 agagattaat gcacgtcatc tgcgcggctc gatggaatcg cgcgggggaa taacccagct
     1681 gcatcgctgc agccagactc agtttcacat attcaggact ttccctttcg agatgggtct
     1741 ctcgattgtt cttatccgag ttcaatgcac ttctaccttc ctgagccaat catcatgaga
     1801 acccttctta cctgctcctt gttcatgacg aggctcatct gggccaggat gatctgtttg
     1861 ccagcgctga tcggaatcaa cgtcaacttt ccgatccgac tgctgaaaac agaacgaatc
     1921 gtgtccatca gttctttgtg ttctatggct tcgataccca aagggagcat gatctccgga
     1981 gtgatctccc tgggtgagtt cccacagagg tcgcacacga atcgattggc gaattcgacg
     2041 cccagatttt cgttcagaag caag
//
LOCUS       DAOQUC010000757         2064 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_68918_length_2064_cov_2.964161, whole genome shotgun sequence.
ACCESSION   DAOQUC010000757 DAOQUC010000000
VERSION     DAOQUC010000757.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2064
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68918_length_2064_cov_2.964161"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1851
                     /locus_tag="PKH07_12300"
     CDS             <1..1851
                     /locus_tag="PKH07_12300"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35768.1"
                     /translation="KPNGYVYERDGVDIIVRPNLTTRIFQLQPDAYKKLEQVLYDPNN
                     VTDPALSLQQILYGDMRKPNVPGKSLRLNPRTMQLIVTDTQENIQKVEEFLRNIPELV
                     QTASPLVTRLFALNPDIAKRLYDIIELMLYEGQKPQAIPQGEEGRQLLLEKDTNVLIV
                     KDTEDNIQKVEEILKDQAILERLQREELVARRFKVSTEDDLTEDGRIRRSNEVKSVEE
                     LLKTMLYTTEGREESIAKGRRMYPNYDQGTIDIVDTPDNVKKVERFLASVRGESQDMK
                     IFPIKHADVDALTRRLNTFSGRRIQGETDYDYDDRFDNQQRIPSRIIPVPESHMIIIR
                     ATGPALDMFTNLVEILDVAPEQVEIETRLIEVGLKDLRQMGIDWTLTNLGEEGLHPGR
                     HDGSERSQLDIGMINQFVPDGMNLAFQILGETELETMLTLLSRAESVNVLSAPKLVTL
                     SSPGDTNPSIDITTSVPFIDSAEVDTHATDTAADDTVIFNYSTEDVGITMEVNPVVSG
                     DGSIIMNVNPVVSVLSGRLPVVTGVPGAVPELGQPIIDTRTASTRVRIRDGETIVIGG
                     LIREEESSTESRVPFLSSIPKIGVLFKDTKTEKDKRTLLIFVTARILKMD"
BASE COUNT          529 a          509 c          563 g          463 t
ORIGIN      
        1 aagcccaatg gctatgtcta tgagcgtgat ggggtggata tcatcgtgcg tcccaatctg
       61 accacgagga tcttccaact tcagcctgac gcctacaaga aactggaaca agtgctctac
      121 gatccgaaca acgtcacgga tccggcgctc agtctgcagc agatcctcta cggtgatatg
      181 cgaaaaccga atgttccagg caagtcgctt cggctcaatc ccagaaccat gcagctgatt
      241 gtgaccgaca cccaggagaa catacagaag gtcgaggagt ttctgcgcaa cattccagaa
      301 cttgttcaga cggcaagtcc tttggttaca cggcttttcg ctctgaatcc ggacatcgcc
      361 aagcgactgt acgatatcat cgaactcatg ttgtacgagg gtcaaaagcc tcaagccatc
      421 cctcagggtg aggaaggacg tcagttgctt ctggagaagg atacgaacgt tctgatcgtc
      481 aaggacaccg aggacaacat tcagaaggtg gaggagattc tcaaggatca agccatcctg
      541 gaacgtcttc aaagagaaga actggtggcg cgacggttca aggtttctac cgaggatgac
      601 ttgacggaag atggccgaat tcgtcgcagc aatgaagtca agagtgttga ggaacttctc
      661 aagacaatgc tgtacacaac agagggtcgt gaggagtcca ttgccaaagg tcgccggatg
      721 tatccgaact atgatcaggg gacgatcgat attgttgaca cgcccgataa cgtgaagaaa
      781 gtagagcgct ttcttgcgag tgttcgtggc gagtcgcagg acatgaaaat cttcccaatc
      841 aagcacgcgg atgtggatgc actgacacgc cggctgaata cattctccgg aagaagaatt
      901 cagggagaga cggactacga ttacgacgat cgtttcgaca accagcagag gattccctcc
      961 cgaatcattc ccgttccgga atcacacatg atcatcattc gagcgacggg gccggccctg
     1021 gatatgttca ccaacctggt ggagattctt gatgtggctc ccgagcaggt ggaaatcgag
     1081 acccgactga ttgaggtcgg cctgaaggac cttcgccaga tgggcataga ctggacattg
     1141 acgaatcttg gcgaagaggg actgcatcct ggtcggcatg atggttccga gcgaagtcag
     1201 ctggatattg gcatgatcaa ccagttcgtt cccgatggga tgaacctggc gtttcagatt
     1261 cttggagaga ccgaattgga gacgatgctc acattgctca gccgtgctga gtcagtgaac
     1321 gtcctttctg ctcccaaact ggtcactctg agttctccag gggacacgaa tccgtccatt
     1381 gacattacga cctcggttcc ctttattgac agtgccgagg tggataccca tgccacggac
     1441 acagccgccg acgataccgt catattcaac tattcgaccg aagatgttgg tatcacgatg
     1501 gaagtcaatc ccgttgtcag tggtgatgga tcgatcatca tgaacgtcaa tcctgtggtg
     1561 agtgttcttt cagggcgttt gcctgtcgtg accggagtgc caggagcggt gccggagtta
     1621 ggccagccca tcattgacac ccgaacggcg agcacccgag tgcgcatccg agacggagag
     1681 acaatcgtca tcggtggtct gattcgggaa gaagagagca gtaccgaatc acgggtgcca
     1741 ttcctcagca gcattccgaa aatcggagtc ctcttcaagg acacgaaaac ggaaaaagac
     1801 aaacgaacgc tgctgatctt tgtgacggca cgtattctga agatggactg atcccaggac
     1861 gatctggtct tgattttgcg cgggtgaggt ttgggcttga cttcaagtcc gaagtgctct
     1921 agttttgtat tgtagttgtc cccgagtgtt tgttcaagaa actgcctacg ggcagctgaa
     1981 tttgcacaca agtaacaacc tcacaatctc caggggcttt actgtcggca aatcttgaca
     2041 caggattcat cattgaactc tgca
//
LOCUS       DAOQUC010000758         2060 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_69117_length_2060_cov_3.432419, whole genome shotgun sequence.
ACCESSION   DAOQUC010000758 DAOQUC010000000
VERSION     DAOQUC010000758.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2060
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69117_length_2060_cov_3.432419"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1401)
                     /locus_tag="PKH07_12305"
     CDS             complement(<1..1401)
                     /locus_tag="PKH07_12305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35769.1"
                     /translation="MKYLVFGALFLLCCSGNSHADSLWHKAESHGRQSQEAIRLCYRY
                     AYGWLSQSDPKTGLLPRNLTGHYYWNSWDAAADNYPFLALTAFVTDQYHLKHSARNIM
                     QQEKRLCSRVGALPWTYDFAKADFVSEVPEMHWVIFGAAEYAKDGLMPMTEFLGKGVW
                     LDRMQDIVRGVWEQAPVETPRGMMPTTNVEVDGDLLQVMSRLYWITGKRSYKRWCYRI
                     ADEYLLYKDMLSWDRIPLRDHGCEIIGGLSETYLIAAQEDPKRRKAYQKPLRAVLDRI
                     LEIGVNADGMMPNGFNPKTGEKNPDELSDGWGYVYNAFLTVAQVDDEPRYREAVSHVL
                     ANVHKYLGDAWEGTGADGYADSVEGALNLLNRLPDDTAFTWVDETMPFLFDKQRSDGI
                     LEGWHGDGNSARTSLMYALWKTQGVSASPWREDLQLGSVLSASGTLLITLKADWAWQG
                     TLRLDRPRHRDYLHLPM"
     gene            1846..>2060
                     /locus_tag="PKH07_12310"
     CDS             1846..>2060
                     /locus_tag="PKH07_12310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016207568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNT35770.1"
                     /translation="MAKDIFKQVVHNVDVCVVGGGMAGICAALAAARRGAKVVLMHDR
                     PVLGGNASSETRVHICGADRSNQIPHMR"
BASE COUNT          465 a          568 c          547 g          480 t
ORIGIN      
        1 catgggcaga tgaagatagt cccgatggcg aggacgatcc agacgaagtg ttccctgcca
       61 tgcccagtcg gctttgagcg tgatcaggag cgttcctgaa gcgctcagca ccgagccgag
      121 ttgaaggtct tcgcgccagg gggatgccga gactccttgc gtcttccaga gtgcgtacat
      181 cagcgacgtt cgagcggagt ttccatcacc gtgccagcct tccagaatgc cgtctgagcg
      241 ttgcttgtcg aagaggaaag gcattgtctc gtccacccag gtgaaggctg tgtcgtctgg
      301 gagacgattc aggaggttca aggcaccctc tacggaatcg gcgtaaccat cggcacctgt
      361 tccctcccat gcgtcgccaa ggtatttgtg aacattggcc agaacgtgac tcaccgcctc
      421 tctgtagcgc ggctcatcat ccacctgcgc aacggttaag aaagcgttgt agacataacc
      481 ccagccgtcg ctcagctcat ctggattctt ctctccagtt ttcggattga agccgttggg
      541 catcatgcca tctgcattga ctccaatttc cagaatcctg tccagcaccg cacgcaaggg
      601 cttctgatag gccttgcgtc gttttggatc ttcctgggct gcgataagat acgtctcaga
      661 gaggccgcca atgatctcac atccatgatc tcgaagcggg atgcgatccc aggagagcat
      721 atccttgtaa agcaagtact cgtcagcaat gcgatagcac caacgcttgt aggagcgttt
      781 gccggtgatc caatacaagc ggctcatgac ttgcagaaga tctccgtcca cctcgacatt
      841 ggttgtcggc atcatgccac gtggtgtctc cacgggtgcc tgttcccaga caccgcgcac
      901 gatgtcctgc attcggtcaa gccagactcc tttgcccaaa aactccgtca tgggcatcaa
      961 tccgtctttc gcgtattccg cagccccaaa gatcacccaa tgcatctcgg gcacttcgct
     1021 gacgaaatcc gccttggcaa aatcatacgt ccagggcaat gctccaaccc gactgcacag
     1081 gcgcttctcc tgttgcataa tattccgggc ggaatgcttc aggtgatatt gatcggtcac
     1141 gaatgccgtc agtgcgagaa aggggtagtt gtctgcagcg gcatcccaac tgttccagta
     1201 gtagtgcccc gtcagattcc gaggcaaaag ccctgtcttc ggatcggact gtgagagcca
     1261 gccgtaagcg tatcggtaac aaagtcgaat cgcctcctgc gactgtctcc catgggattc
     1321 ggctttatgc caaagggagt ctgcatgcga attcccgctg cagcacagta agaagagcgc
     1381 tccgaaaaca agatacttca tcccagcagg cctcctctga gaattgagtt tctcatctta
     1441 tatgatttca acgatcccct tcaagcattt ttcggttgaa ttgttccctg ccaagggtta
     1501 tagttcgttt tccgtcagaa tgggattttc cctctgacca gcagaaacgt ttgaagaact
     1561 tacgaccttc tgcccaaatt gagaagggag ggtgagtatg ccgtccggaa agaaacggaa
     1621 acgtcaaaag atcgccactc acaagcgcaa aaagagacgg cgacgtgatc gtcataagaa
     1681 gaagtaatcc cgctcgttga gagcgtgaga agcctattgg agtacgggca cggttttctg
     1741 ccgtgtccgt actctttgtt cttgacagac acatccatct gcatcagctt tcccagagtg
     1801 tatccctgtt gattttgacg acatcacgaa gggagtattc gagggatggc aaaggacatt
     1861 ttcaagcagg ttgttcacaa cgtggatgtt tgtgttgtcg gtggtggcat ggcgggcatc
     1921 tgcgcggcgc ttgcggcagc gcggcgcggc gcgaaagtgg tgctgatgca tgaccgaccg
     1981 gttctcggcg gcaatgcttc cagtgaaacc cgcgtccata tctgcggtgc cgaccgaagc
     2041 aaccagattc ctcacatgcg
//
LOCUS       DAOQUC010000759         2060 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_69134_length_2060_cov_2.898254, whole genome shotgun sequence.
ACCESSION   DAOQUC010000759 DAOQUC010000000
VERSION     DAOQUC010000759.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2060
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69134_length_2060_cov_2.898254"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            169..960
                     /locus_tag="PKH07_12315"
     CDS             169..960
                     /locus_tag="PKH07_12315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35771.1"
                     /translation="MNECLTAQGMPAFDPDAIPDCGVVIEMPILEGNWSLRTVLDVLA
                     VMAGFWNWGVDLKDNVYFQPTPDSIQAQFHVGMERGWGKVVSLDDRYCPYETLTSIKV
                     YGEVPVSLGENGQIVRPEHRTAFSKSFPDPTMTNTYGNNQRVVHVPMVRAEKQAAAYA
                     NACLTRVNATSHKYELHVSNALRPLDPACGLIMLHDEVGERLGELPAERIIFELGEDA
                     HVHIHLGQPDLFTDNLSLLPHKAEKGVLPDETRILSSILDGEKAS"
     gene            1004..>2060
                     /locus_tag="PKH07_12320"
     CDS             1004..>2060
                     /locus_tag="PKH07_12320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35772.1"
                     /translation="MKSATVLLLLVLGIAVLVCSARAGLFGSDGRVGSWRGCQSLVES
                     PTGEHLIFCDVSSGTHTLSGLWNGGGGGLGPYLRDDQADIMRAATASLTIDATTPEAS
                     QPILTLCAQERNLAVFNCSGELWLNGYLKQPSGVVRQFGDESDEYWDSDEKSLTWRVC
                     ATPAAEVDFDDRYLGVRGGIGAHNYTILSPDSGTVRFFENGGSYTEYWDATENALSLR
                     ASATPAASVDLDNGNMSLAGNLSLMADRKVTQPEDTTGNGPFILSGLVRASLSGFTVN
                     TQAACYFTTTTGSLVLGAIPEEIYGGVVVVDDLVIHGCTGSLHAYIDSVELRHTDRVN
                     SSFSVVTYTTDIGNDTDG"
BASE COUNT          436 a          534 c          600 g          490 t
ORIGIN      
        1 gtcggtcgtg ttgcaggtgt tcggcgacgg acaagtggtg gggagttcga agtgatccta
       61 ttgtcccctg tgctttgtct cgaaggcgtt tggcctgttt tcccgagccc tgcggcagcg
      121 tctgagcatc cttcagaggc ggatgtttct tattgagttc ggagtttgtt gaacgagtgt
      181 ctcactgcac aggggatgcc tgcattcgat cctgatgcga tccctgactg cggcgtcgtc
      241 atcgagatgc cgatccttga ggggaactgg agcttgcgta cggtcttgga tgtcctcgcc
      301 gtgatggcag gtttctggaa ctggggagtg gatttgaaag acaacgtcta ctttcaaccc
      361 acccctgatt ccatccaagc tcagttccat gtcggcatgg agcgggggtg gggaaaggtt
      421 gtgtctttgg acgatcggta ttgtccgtat gagacgctca cttccatcaa ggtctatggt
      481 gaggttcccg tctctttggg cgaaaacgga caaatcgttc gacctgaaca ccgcacggcc
      541 ttcagcaaat catttcctga tcccacgatg acgaacacgt atgggaacaa ccaacgcgtt
      601 gttcacgttc cgatggtgag ggcggaaaag caggcggccg catacgcgaa cgcctgcctc
      661 acgagggtaa atgccaccag tcacaagtat gaactccatg tctccaatgc tttgcggcca
      721 cttgatcccg cctgtggcct tatcatgctg catgatgagg ttggagaacg actcggagaa
      781 ttgccggccg agcggatcat ctttgaactc ggtgaagatg cgcatgttca tatccacctc
      841 ggtcagccag atctctttac cgacaaccta tcactgctgc cccacaaagc ggagaagggc
      901 gtccttcccg acgaaacacg aattctctca agcatcctgg atggtgagaa ggcgtcctga
      961 cgatgattca gaaagatcac agttctcagg gaggttacag aatatgaaat ccgcaacagt
     1021 cttgctcttg ttggttttgg gaatcgccgt tttggtctgc agtgcccgtg ccggtctgtt
     1081 tggcagcgat ggacgggttg gaagctggag gggttgccag tcgcttgtgg aatcgcccac
     1141 gggcgagcat ctcatttttt gtgacgtttc ttcaggcaca cacacgctga gtggtctgtg
     1201 gaatggcggt ggcggtggtt tgggtcctta tcttcgagat gaccaggcgg acatcatgcg
     1261 cgctgccacc gcatcgctga ccattgatgc caccactcct gaagcctctc aaccgattct
     1321 gacactttgc gcacaggaac ggaatctggc cgtcttcaac tgctccggtg aactctggct
     1381 gaacggctat ctgaagcaac cgtctggagt ggtgcgacaa ttcggggacg agagtgacga
     1441 atactgggat tcggatgaga agtcgctgac ttggcgggtg tgtgcaacac ctgccgccga
     1501 agtggacttc gatgatcggt atctcggagt acgcggtggc atcggtgctc acaactacac
     1561 aatcctcagc ccagattccg gaacagttcg gtttttcgag aatggtggct cttacacgga
     1621 atactgggat gcaacagaga acgctctgtc gctcagagca agcgccactc cagctgcttc
     1681 agtggatctc gacaatggca atatgtctct tgcgggcaat ctttcgttga tggccgaccg
     1741 aaaggtgact cagccagaag acacgacggg aaacggtcct ttcatcctga gcggcctcgt
     1801 gagagccagt ctgtcgggat tcacagtcaa cacgcaggcg gcttgttatt tcaccacgac
     1861 aacgggcagt cttgtcttgg gagctattcc cgaagaaatc tacgggggtg tggtggtggt
     1921 ggacgacctg gtaatccatg gttgcaccgg ctccctgcac gcctacattg acagcgtcga
     1981 actgcgacac acggatcgag tcaactccag cttcagcgtt gtcacataca ctacggacat
     2041 cggcaacgat acggatgggg
//
LOCUS       DAOQUC010000760         2059 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_69185_length_2059_cov_3.111277, whole genome shotgun sequence.
ACCESSION   DAOQUC010000760 DAOQUC010000000
VERSION     DAOQUC010000760.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69185_length_2059_cov_3.111277"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..522
                     /locus_tag="PKH07_12325"
     CDS             <1..522
                     /locus_tag="PKH07_12325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35773.1"
                     /translation="EPNRISERPRQPESLREVPDSGSRLPNFVPKQVQEPERVAPRAP
                     SVEPRMNPSEPVRFGNEPRTSAPKQPERDSRPVESYNVRPSEPSRAQPNVPSRPDRSV
                     SRPVAPRPEAPSIDIQAPTRQEPPTSVPQSRSVEQPSRSFQPDGRSSSVVQPPSGRTE
                     SPEQGRGQFGRSR"
     gene            complement(601..691)
                     /locus_tag="PKH07_12330"
     tRNA            complement(601..691)
                     /locus_tag="PKH07_12330"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(655..657),aa:Ser,seq:cga)
     gene            complement(767..1222)
                     /gene="tadA"
                     /locus_tag="PKH07_12335"
     CDS             complement(767..1222)
                     /gene="tadA"
                     /locus_tag="PKH07_12335"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013840114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HNT35774.1"
                     /translation="MTTAVIDQYMRAALVEAQEAFEQGEVPVGAVVVLNGKIVGQGHN
                     QRERLADPTAHAEILALRQAAQACGSWRLSGADMYVTLEPCTMCAGAIVLARVRRLFF
                     GADDPKAGAVVSLFRILDDNRLNHTVEVFKGVLEDECSGMLSRFFATKR"
     gene            1354..>2059
                     /locus_tag="PKH07_12340"
     CDS             1354..>2059
                     /locus_tag="PKH07_12340"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HNT35775.1"
                     /translation="MKLSSLQKKLLVMITVCCCVILLACHRRPVPNSPNVLLVTFDTV
                     RADHVGCYGDPRGLTPTVDHLASQGIIFRQCTSPAPITLPTHASILTGLYPFHHKVRN
                     NGTYKLMPDVQTLGSILHLAGYDTAAFVSAHVLKAQYGLARGFDVYDDHVAESSTGMT
                     SEIAERAATDTTDAVLKWIAGRSSQRPFFLWVHYFDPHMAYNPPQLWRDRFPDDPYSG
                     EIAYADEQLGRVLEQID"
BASE COUNT          461 a          594 c          542 g          462 t
ORIGIN      
        1 gagccgaaca ggatttctga gcgtccacga cagcctgaga gtctgcggga agtgccagac
       61 tccggaagtc ggttgcctaa ctttgttccc aaacaagtac aggaacctga acgggtggct
      121 ccgagggcac caagcgttga acctcggatg aatccttctg agcctgtgcg atttggcaac
      181 gagccgagga caagtgctcc gaagcagccc gaaagagaca gcagacctgt ggagtcttac
      241 aacgtcagac cgtcggagcc aagtcgggcg cagccgaacg tgccatctcg gcctgaccgt
      301 tcggtcagca gaccggtcgc accccgacca gaggcaccaa gcattgacat tcaggctccg
      361 acacgacagg agccgccaac gtcggtgcct cagagtcgtt cggtggaaca gcccagccgc
      421 tctttccaac ccgacggacg tagttccagc gtggtgcagc ccccctctgg acgaactgaa
      481 tctccagagc aaggccgcgg ccaattcggc agaagccgat aggttgttga ttcgatcact
      541 gagagtgtca tgaaaaggga atcccggttg aaaggccggg attcctcttt ttcgataggc
      601 tggcggagag gcggggattc gaaccccggg tgggctttag acccacagac gctttcgagg
      661 cgcccgcctt cgaccgctcg gccacctctc cgctgatgtc cttggagtat cactcccaag
      721 gtctatttta gagcgttggt gatcctacga tacatcagaa cctatttcaa cgcttagttg
      781 caaaaaaacg ggaaagcatt ccgctgcact cgtcttcgag aactcccttg aacacttcaa
      841 cggtatgatt tagtcggttg tcatctagaa ttcggaacaa actcactaca gcaccggcct
      901 tgggatcgtc ggcaccaaag aaaagccgcc gaacccgtgc gagaacaatc gcgccggcgc
      961 acatcgtgca aggctccagc gtcacataca tatccgctcc gctcagacgc caactaccac
     1021 aagcttgggc tgcctgtcgg agtgcaagga tttcagcatg agcggttggg tcggcaagtc
     1081 gctcacgctg gttgtgacct tgtccgacta tcttgccgtt cagaacaaca accgcgccga
     1141 ccgggacctc tccctgttcg aaagcttctt gggcttccac gagtgctgca cgcatatact
     1201 ggtcgatcac tgcggttgtc atcgtatcca tccttcctca atagggaaac tatgctatca
     1261 ccgttttcgg ttgatgtgac ggaaaattcc tttgccttcc tgtcttgacc gaatcgtctc
     1321 acttcgagat actctccgtg ttggaggata cctatgaaac tcagttcgtt gcagaagaaa
     1381 ttgcttgtga tgataactgt ctgctgctgc gtcatccttc tcgcctgcca taggagacct
     1441 gttcccaaca gcccgaatgt tcttcttgtg actttcgaca cagtccgcgc cgatcacgtc
     1501 ggatgctacg gagaccccag agggttgacc ccgacggtcg atcatctggc gagtcagggg
     1561 atcattttca ggcaatgcac cagtccggct ccgatcacac ttccaacgca cgcatccatt
     1621 ctcacaggtt tgtatccctt tcatcacaag gttcgaaaca acggcaccta caaactgatg
     1681 cccgatgttc agacgctggg aagcattctt caccttgcag gatatgatac ggctgccttt
     1741 gtcagcgctc atgttctgaa ggcgcaatat gggcttgcac ggggttttga tgtctatgac
     1801 gatcacgtcg ccgagagttc aacgggaatg acatctgaga ttgctgaacg tgccgcgact
     1861 gataccaccg atgccgttct gaaatggatt gcaggccgtt ccagccagcg accgttcttt
     1921 ctttgggtgc actatttcga tccccacatg gcctacaacc ctccgcaact ttggcgggat
     1981 agatttccgg acgacccgta ttccggcgag attgcgtatg cggacgagca actgggacga
     2041 gttttggagc agatcgact
//
LOCUS       DAOQUC010000761         2057 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_69280_length_2057_cov_3.733766, whole genome shotgun sequence.
ACCESSION   DAOQUC010000761 DAOQUC010000000
VERSION     DAOQUC010000761.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2057
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69280_length_2057_cov_3.733766"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..867)
                     /locus_tag="PKH07_12345"
     CDS             complement(<1..867)
                     /locus_tag="PKH07_12345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008317631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HNT35776.1"
                     /translation="MDWKSQWKRLAILTGLFLVFFWLPVDYERFNNAIMESLELSRWY
                     AREHVLLCLVPAFFIAGAIGVFVSQASVMKYLGARANKVLAYGVASVSGSVLAVCSCT
                     VLPLFAGIWRMGAGLGPACAFLYSGPAINVLAIILTARILGLQLGIARAVGAISFSVI
                     IGLIMHLVFRKEEIAKSNAQMAMAEPEVSRPLWQNALFFAVMIGILVFANWGAPNDFL
                     FELQDGRILQAAVIKAPEEDGVGEYRLKILTGSMEGQEMLIRAQEVVSRRPVLGAWTT
                     IWQNKWIITSAFG"
     gene            1100..>2057
                     /gene="sat"
                     /locus_tag="PKH07_12350"
     CDS             1100..>2057
                     /gene="sat"
                     /locus_tag="PKH07_12350"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF003166.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase"
                     /protein_id="HNT35777.1"
                     /translation="MIKPHGGRLIDRMATDKERESLTEQISKMPTIVLNAREISDMEM
                     LATGAMSPLEGFMTKEDYLSVLDLKRLAKGLPWTIPVTLSVKDGDGSQYKVGKDIAMT
                     DEQGTVFGVLHLEEKFKVDRHYEAERVLLTNDPSHPGVQYLDTLTDIYLGGQITVLQR
                     PKHEKFNNWRLDPKETRVLFKTKGWNSVVAFQTRNPIHRAHEYLQKCALEICDALLIH
                     PLMGETKSDDIPADVRMECYLTLMKEYYPSTRVALSVFPAAMRYAGPREAIFHALVRK
                     NYGCTHFIVGRDHAGVGSFYGTYDAHYIFREFEPQELDITPLF"
BASE COUNT          539 a          572 c          513 g          433 t
ORIGIN      
        1 tccaaaagcc gacgtgatga tccacttgtt ctgccagatg gttgtccacg cacccagcac
       61 aggccttcgg gaaaccacct cttgggcacg aatgagcatc tcctgtcctt ccatagaacc
      121 ggtcagaatc ttgagtcggt actcgccaac accgtcttct tcaggagctt tgatgacagc
      181 ggcttgaagg attcggccat cctgaagttc aaataggaaa tcgttcggag ccccccagtt
      241 ggcgaaaacc agaataccaa tcatgaccgc aaaaaagaga gcgttctgcc agagaggtcg
      301 tgacacttcc ggttccgcca ttgccatctg ggcattcgat ttcgcgatct cttccttgcg
      361 gaacaccaaa tgcatgatca atccgatgat cacgctgaaa ctgatcgctc ccacagcacg
      421 agcaatgccc agttgcagtc ccaggattcg agccgtcagg atgatggcca aaacgttgat
      481 tgccggaccc gaatacagaa aggcacacgc ggggcccaac cctgcaccca tccgccagat
      541 acctgcaaag agaggcagaa ccgtgcaaga acaaacggcc aggaccgaac cggacacgga
      601 cgccactccg taggccagga ctttgttcgc tcgagcaccg aggtacttca tcacggaggc
      661 ctggctgaca aataccccta tcgcaccagc gatgaagaac gctggaacca gacataagag
      721 cacgtgttct cgggcatacc agcgactcaa ctcgagtgac tccataatag cattgttgaa
      781 tcgttcatag tccacaggaa gccagaagaa aaccaagaac aagcctgtca atatggccag
      841 tcttttccat tgggatttcc agtccataag ccagcccgcc tttctacaac accatcatta
      901 ttggcaatat gaccaaatag ccaaatagtt gtcaagtcat ttcattccag cgaacaaaat
      961 cctttgtacg ggttgacaga accgctcctg ttcccctaag ttacttccct catgcccgag
     1021 agattcagac agcgcagggg cagcagcccc atttgttgtt ggtcttatcg gcaagcaatc
     1081 tgaagatgga gaacaaaaaa tgatcaagcc ccatggtggc agactaattg accgaatggc
     1141 gaccgataag gaacgcgaat ccttgaccga gcagatttcc aagatgccga caatcgtact
     1201 gaacgctcgt gaaatcagcg acatggagat gctcgccaca ggagccatga gcccgctgga
     1261 aggtttcatg accaaggaag actacctgag tgttcttgac ctgaagcgcc tcgcaaaggg
     1321 tcttccatgg acgattccgg tgaccttgtc tgtcaaggac ggggacggtt cccagtacaa
     1381 ggtcggcaaa gacattgcaa tgaccgacga gcagggaact gtgttcggcg ttctgcacct
     1441 tgaggagaag ttcaaggttg acagacacta cgaggccgag cgggtgcttc tcaccaacga
     1501 tccatcccat cccggcgtac agtatctgga caccctgact gatatctatc tgggcgggca
     1561 gattaccgtt ctccaaagac ccaagcatga aaagttcaac aactggcgtc tggatccaaa
     1621 ggagactcgt gtgctgttta agaccaaggg ttggaacagc gtggtggcct tccagacacg
     1681 caatccgatc catcgagccc atgagtacct tcagaaatgt gctctggaaa tctgcgatgc
     1741 actgctgatt catcctctga tgggtgaaac caagagtgat gacattccgg cggatgtccg
     1801 tatggaatgc tatctgacgc tgatgaaaga gtactatccg tccacccgag tggcgttgag
     1861 cgtattcccc gcagccatgc gttacgccgg accgcgtgaa gccatcttcc atgcgctggt
     1921 gcgcaaaaac tacggttgca cccatttcat tgttggtcga gatcacgccg gtgttggcag
     1981 tttctacgga acctacgacg cacactatat cttccgtgag tttgagccgc aggagctgga
     2041 catcaccccg ttgttct
//
LOCUS       DAOQUC010000762         2057 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_69304_length_2057_cov_2.258242, whole genome shotgun sequence.
ACCESSION   DAOQUC010000762 DAOQUC010000000
VERSION     DAOQUC010000762.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2057
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69304_length_2057_cov_2.258242"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            16..2057
                     /locus_tag="PKH07_12355"
                     /pseudo
     CDS             16..2057
                     /locus_tag="PKH07_12355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008676413.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
BASE COUNT          439 a          562 c          606 g          450 t
ORIGIN      
        1 aacagcctga ccaccaaaga acgtctggag ctgttcatgt cggtctgcca ggcggtgcag
       61 catgcccatc agcgggggat catccatcgg gatatcaagc cgtcgaacat cctggtcaca
      121 ctgcacgatg ggcagcctgt accgaaagtc atcgactttg ggatcgccaa ggcggtgaac
      181 cagcaactga cggagaagac ggtcttcaca cgctactccc agatgatcgg cacgccggag
      241 tacatgagtc cggaacaggc ggaaatgagc ggcctggaca tcgatacgcg aacggacgtg
      301 ttctccctgg gcgtgctgct atacgagttg ctgaccggca caactccttt cgattccgaa
      361 tacttgctga gcaagggcta cagcgagatg cagcggatca tccgggaaga ggagccgctg
      421 cgtccgtcca ccaaagtcag tacgctgggt gagactctca cggatgtggc gaaacaccgc
      481 cggacgagtc cggagttgct gtgcaaactg attcggacgg acctggactg gatcgtgatg
      541 aagacgctgg agaaggaccg caaccgccgc tacgagtccg tgagcgaact ggccgccgat
      601 gtcaaacggc atttggatga tgaacccgtc ctggccgggc ggcccagtac cgtctacaga
      661 gtacagaagt ttgtcaaacg caataggacc ctctgcatgt catccgttgc catcgccggt
      721 atcctcatcc tggcggttgt tgtttcatca caccaggcat gggtggcaca aaaggcccga
      781 agcgacgaaa gctcggctcg tgcaactgcc gagctggagc gtgatcgagc acgagcagct
      841 caacaacaag ctcaacaaga gcgtgtccag gcccagagtc atctttatga ctctctcgta
      901 cgtgaggcac aactcacctg cattactcgt ggtctggggt atcgcgaacc ggtctttcgg
      961 aaccttcaac aggcccgaga ccttgatgtg ccgaataagg acttggtggg gcttcgcaaa
     1021 gaggccattg cgtgcctagg tgacttcgta ggcaaagagc ctaaattggt gttggaggtt
     1081 cctgaagagc ctgagtcgct tgtgattgtg tggggaacgt tgcatccgag cgacgcaatt
     1141 gcagcgttcg ccctctctga tgggagcatt ctgctcaagg acctgcaatc cacgaaagat
     1201 atcgccaggt tccagtgcga acactttcca tatggtatgt gtttcgccaa cactggcaac
     1261 tcacttctct cgctccacgt gcccaacggc tattcccctg agccacggct tgccgacgcg
     1321 gtgtcacacc tgtttgtacg ggttcaagat ggtacgtggg tcgaaggaag gactcttgag
     1381 gtccccaacg ctgtatgttg tatgtccaca gcggctggtt tcagcgttgc cgtcgcggac
     1441 gtggcgtcgg gctcggctca tctggtggaa ccactgacgg gcggcatcac acacgagttc
     1501 ggtctccgcg tggatatgat ggttctccca cctatcgcac tgaccgtgga cgggtgtttg
     1561 ctggcggctg gcacgaacga cccgaacagt cccaacacgt gtgtcgtgga cgtatgggat
     1621 ttgaagctga acgagcgtct cgctcggctg gaacctggct tggcggggtg cgcccgttta
     1681 cgattcagcc cggatcggcg atacttggca ttcctttcgg agtcaggggg cattgtatac
     1741 tccacggata cgttcgaacc cgtggcccat ttcaaggacc aatttgccat ggattctgac
     1801 ggtgtctttc tcccaggcag tgatgtgctg gttctctcct tgaaatcgtc acaagtgctc
     1861 ttctgggatt gtgcaaggaa ggagaatgtc gcgacatttg aacaaccttt gggagatgcg
     1921 cgatggattt ccacgtctgc tgatggaaga acgatgatga gctatcacta caagcgggcg
     1981 tggctgtact cactcgatac caccgttgaa agactgactt tttctgcgca ctctgccggt
     2041 gtgcccggga tcgcctt
//
LOCUS       DAOQUC010000763         2054 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_69463_length_2054_cov_2.747874, whole genome shotgun sequence.
ACCESSION   DAOQUC010000763 DAOQUC010000000
VERSION     DAOQUC010000763.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2054
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69463_length_2054_cov_2.747874"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="PKH07_12360"
     CDS             complement(<1..253)
                     /locus_tag="PKH07_12360"
                     /inference="COORDINATES: protein motif:HMM:NF016430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNT35778.1"
                     /translation="MTNLRLELERFYEDNEQQLFTFALSMTRDHDGAEDAVHEAFSRL
                     LQMGGCPDNLKSYVFRSVRNAAIDGMRKTARLCELTDEGA"
     gene            806..901
                     /locus_tag="PKH07_12365"
                     /pseudo
     CDS             806..901
                     /locus_tag="PKH07_12365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003112842.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
     gene            complement(1500..>2054)
                     /locus_tag="PKH07_12370"
     CDS             complement(1500..>2054)
                     /locus_tag="PKH07_12370"
                     /inference="COORDINATES: protein motif:HMM:NF024770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNT35779.1"
                     /translation="VPGLDAPAVVSDDVSGGYLAAEHLVKQGHHRIAAVFPPPYSTAR
                     AREDGFRKALEDNGVEVRNEWIQYACTDDPEPEKTMVEALFSQAERPTAIFAFHDELA
                     AAVFNHLKTRGLRVPEDVALVGYNDCVIAMTLPVPLTSVAYPKYEIGANAARLLLRGA
                     RTASETIVLPPRLVVRASSVKKPK"
BASE COUNT          510 a          565 c          518 g          461 t
ORIGIN      
        1 gcgccccttc gtcggtcagt tcgcagagtc gcgccgtctt cctcatgccg tcaatcgccg
       61 cgttgcgcac cgagcgaaag acataagact tcaggttgtc aggacaaccc cccatctgaa
      121 gaaggcgact aaaagcttca tgaactgcgt cttcggcacc gtcgtggtcg cgggtcatcg
      181 agagggcgaa ggtaaaaagc tgctgctcat tatcttcgta aaaccgctcc agttccagcc
      241 tcagattggt catgcctttc atcctctatc gggaggggct tgcgctccat ccgtcatcgt
      301 caatagagag acggacgagc gacctctttt tgttcataga atccttgggc acaaaagcac
      361 gcgattccag ccgcaacact gaatctgcgg ctgtcccgag tcgaaagata ctgctgataa
      421 ccgatggcgg aggaggcaaa agaaattctg agggctgctg atttaccccg catccatgaa
      481 ctcatcatgt tgttaagtct cccatattcg aggcggctgg tcaaattcct caagaggtgg
      541 tgcgtctcgc cccacacgga ttttgtcggg ggcaagatgc tcccgctcct ttgtgcaggc
      601 aagatgcctg cgccacaacc agattgtcga gcagacattc gccgatcccg cccaccgttt
      661 gactcagagg agcaagagtt caacggctgg aaacaaccca gacaatccgt ccaaagccat
      721 ctgacttttc ttcttccttc gatcggggat gaagagtata ctgattttgt gtgatagaat
      781 acccctatgg aaaggaggtg cgaagatgag acatgcggaa agaaaaggtt ttactctgat
      841 cgaactgctt atcgttgtgg caatcatagg aattcttgca gccattgcga ttccaaactt
      901 ccttctcgca cagactcgag caaagatttc tcatgctcga tccgaggtac aagcttttgc
      961 gaccggtctt gaggcttatt ttgtggacaa caattcctac ccaccgatga gcgatacgac
     1021 atcggacagt acgccgactg tttttgacaa cgaacatgct cgaatgccca acttcttgac
     1081 aacacctgtg gcctacgtca agcaggtgtt cacagatccc tttgtggaat tcgacaaggt
     1141 actcccacct tttggtgtcc gatacaccta tttcaactac gatcaataca tcaactggat
     1201 gggctggact ggaaccatcg tgcgcagggc acacgccgca ggcaaatggc tcatttacag
     1261 ctggggagcc gatcatacga tgaacaacac agcggtggat ggtgtctata ccaactacga
     1321 tcccagcaac ggcgtcatca gcattgggaa catcattcga acccaacgcg acccatcacg
     1381 atacagaaca gaagactact gattcacaaa agattcacac gcattacaga gagactcaac
     1441 atctgttggg tctctttgcc tatgaaggat gcgcgcagaa gcatcagcgt ccatgctgtc
     1501 tattttggct tcttgacact ggaggctcga acgaccagac gaggtggcag aacaatggtc
     1561 tccgaagcgg ttctggcacc gcgcagcaag agccttgccg cattggcgcc gatctcatat
     1621 ttcggatagg caactgaggt cagaggaacc ggcaacgtca ttgcaatcac acaatcgttg
     1681 tagcccacaa gcgcgacatc ctcaggaaca cgaagccctc gtgtcttgag atggttgaag
     1741 actgccgcgg ccagttcatc atgaaaggca aagattgccg tcgggcgctc cgcctgagaa
     1801 aacagcgctt ccaccatagt tttttccggc tcaggatcgt cggtgcaggc gtattgaatc
     1861 cattcatttc gaacttctac gccattgtct tcaagcgctt tgcgaaaacc gtcctcacgc
     1921 gctctggcgg tgctataggg aggcgggaat accgcggcaa ttctgtgatg accctgcttg
     1981 acgagatgct ccgcggcaag atatcccccg gagacatcgt cggaaacgac cgcgggagcg
     2041 tccagacccg gaac
//
LOCUS       DAOQUC010000764         2053 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_69497_length_2053_cov_3.256256, whole genome shotgun sequence.
ACCESSION   DAOQUC010000764 DAOQUC010000000
VERSION     DAOQUC010000764.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2053
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69497_length_2053_cov_3.256256"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            223..792
                     /locus_tag="PKH07_12375"
     CDS             223..792
                     /locus_tag="PKH07_12375"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNT35780.1"
                     /translation="MFLGQKRVVWVLGLVLLVCCFSGCRCRKPLPPPVGPTDAGTTQQ
                     TDQTGYQPDSTGVSQTDTIPRVDAQVEEIEVPELKMVHFDYDRAEVRAADEPILRANA
                     EWLKANMQTFDMIKIEGHCDERGTNKYNMVLGERRANAVREYLGSQGIPLSIIIPVSY
                     GEERPLDPGHTEASWAQNRRAEFRIVVEK"
     gene            911..1495
                     /locus_tag="PKH07_12380"
     CDS             911..1495
                     /locus_tag="PKH07_12380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012507058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNT35781.1"
                     /translation="MIGLNRSVSVGLLLVVVVCSMMSGCCCRPRPEKPEVAPPAVAPT
                     PKPKPAEPTPGRIDEGAVVVPDPNLKPIYFDFDKYVIRPADAEILKSNAAWIKENMGK
                     GVFKQILVEGHCDERGTNKYNMVLGERRANAAKNFLINEGIPADILVAVSYGEEKPAD
                     PRSNEEAWAKNRRSEFKKVLRDLNMDDLDLPNIQ"
     gene            complement(1648..>2053)
                     /locus_tag="PKH07_12385"
     CDS             complement(1648..>2053)
                     /locus_tag="PKH07_12385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019499808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNT35782.1"
                     /translation="IRLEMTESELMEQAQSAVEKLEALKKLNIQLYIDDFGTGYSSLS
                     YLHQFPIDALKIDRSFIQSLKPGDEESGIVKTIVLLARDLGLSVIVEGIETAEQLEII
                     RKLKCQYAQGFFFSKPLPPAEIETLLERNPQW"
BASE COUNT          475 a          512 c          580 g          486 t
ORIGIN      
        1 agggaagcaa tgagagtccc tcttgggctc ccgattcaca gcaccttgtt ttcagttcaa
       61 cccgagaggg ttcttcacag ttgtacgtga tggacattga cggtcaggat gtcaggcgtt
      121 tgacgtatct cgctggagac aatcaatcgc cgggctggtc gccggtgcct tcgcagtaga
      181 tcacctcagg agagcatcca gaaaaaggag gacgatgagt tgatgttcct aggtcagaag
      241 agagttgttt gggtgttggg tttggtgctt ttggtgtgct gtttttcggg ctgccgctgc
      301 cgtaagccgc tgccgcctcc cgttggtcca acagacgcgg gaacaacaca gcagacggat
      361 cagacgggct atcaacccga ttcgactggt gtgtcgcaga ccgacacgat tcctcgagtt
      421 gacgctcaag ttgaggaaat cgaggtgcca gagctgaaga tggtgcattt cgactatgat
      481 cgcgctgaag ttcgtgctgc cgatgagccg atcctgagag ctaacgcgga atggctcaag
      541 gcgaacatgc agacattcga catgatcaag atcgaaggcc actgcgacga gcgaggcacc
      601 aacaagtaca atatggtgct gggtgaacga cgcgccaatg ccgttcgaga gtatcttggc
      661 agccagggaa ttcccctgtc gatcattatt ccggtctcct acggcgaaga gcgtcctttg
      721 gatccaggac acaccgaagc ctcttgggcg cagaatcgcc gagcggaatt tcggattgtt
      781 gttgaaaaat agatcttgtt gtagtacctt cccatcgggc tggtgaaagg atgccagtcc
      841 tatttggctg ttgaacttct ttcaaacagc aaaggtggat gtattacttt caaaaccagg
      901 aggaaagagt atgattgggt tgaatcgttc tgtgtctgta gggttgttgt tggtggttgt
      961 cgtgtgctcg atgatgtcgg gctgctgctg ccgaccccgt ccggagaaac cggaagtagc
     1021 tccgcccgcc gtggctccga caccgaagcc gaagccggcc gagccgactc caggacgtat
     1081 tgatgaaggc gctgtagtcg ttccagatcc gaatctgaag ccgatctact ttgacttcga
     1141 caaatacgtc attcgtcctg ccgatgccga gatcctgaag agcaacgctg cttggatcaa
     1201 ggaaaacatg ggcaagggtg tcttcaagca gattctcgtc gaaggccatt gcgacgagcg
     1261 aggcaccaac aagtacaaca tggttttggg tgagcgacgc gccaatgccg cgaagaattt
     1321 cctgatcaat gaaggcattc ctgcggacat tctcgtggcg gtctcctacg gcgaagagaa
     1381 accagccgac ccgcgtagca acgaggaagc ctgggcgaag aaccgtcgtt ctgagttcaa
     1441 gaaagtcctt cgtgacctga acatggacga cctcgatctg cccaatatcc agtaagcgaa
     1501 tcgggtttga tcaaaccacg tctatcaacc gctgaccggc gcaatgcgct ggtcagcggt
     1561 ttccgtttga tacttgcctg acggcgatgc tgtcgcgcac ggtgcctcaa gagccttcag
     1621 agactaagat tctgacgatg agggtcttta ccactgcgga ttgcgttcca ggagcgtttc
     1681 gatttctgcg ggggggagag gctttgagaa gaagaatccc tgagcatatt gacacttgag
     1741 tttgcggata atctcgagtt gctccgcagt ttcgattcct tcaacaatga cactgagtcc
     1801 caagtctcgc gccaaaagga ctattgtctt gacgattccc gactcctcat cgcctggctt
     1861 gaggctctgg atgaaggaac gatctatttt gagtgcgtct atcgggaatt ggtggagata
     1921 actcagtgac gaataccccg tcccgaaatc gtcaatatag agttgaatat tcaacttctt
     1981 cagcgcttcc agtttctcaa cagcagactg cgcctgttcc atcagttcgc tctcggtcat
     2041 ctccagccga atg
//
LOCUS       DAOQUC010000765         2050 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_69673_length_2050_cov_2.824060, whole genome shotgun sequence.
ACCESSION   DAOQUC010000765 DAOQUC010000000
VERSION     DAOQUC010000765.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69673_length_2050_cov_2.824060"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..133
                     /locus_tag="PKH07_12390"
                     /pseudo
     CDS             1..133
                     /locus_tag="PKH07_12390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013034282.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
     gene            complement(344..571)
                     /gene="raiA"
                     /locus_tag="PKH07_12395"
                     /pseudo
     CDS             complement(344..571)
                     /gene="raiA"
                     /locus_tag="PKH07_12395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
     gene            815..>2050
                     /locus_tag="PKH07_12400"
     CDS             815..>2050
                     /locus_tag="PKH07_12400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35783.1"
                     /translation="MLAILRTMLPIVLLCLCQLAPCAVLNVPARRGDAMSGSAFVTAI
                     TPLSLTERENAILSEVMLGNVPDFMRNLKPVTVSATINSQLRTVIYHVLPDYLAVGSD
                     ADYFLTPMSPLCAQRIADRLNCLLPTRKMVNDIWSQAQVKLSPSTIPPSPEMTTVPVF
                     AQHNTTVRAQRQAMEVQYPLGALVGGDKKDVVITKLLATTPNKVAIYGWHYTNGTPIQ
                     PLYLGHEDTYADYSHGIRLVQQAVTLDGVETTAVQILNDASLNVLLSDEGVVTSTRYP
                     VALPLYFPLQDDFPASGRDSLLWTDKFKTPTLMSFSPTSPGGDGYALRVMDSAGGMET
                     TRTGNITDADYYVQCDIYCTYRPALVSDGFERSGIFLRDNGNGAFEHTTGGGGYCYAM
                     AWDSGNGRLWCLKSVNGVIT"
BASE COUNT          434 a          579 c          539 g          498 t
ORIGIN      
        1 gcacgaatgg gtggcggcgc agccgaagga tgtggtcttc gtgcgcatac ccgccgtgtt
       61 ccgccccagt tgggaaccgt tggcgcgcgc ctactacacc gccgagatgc ttggcgtgct
      121 cgataagatc catctgcatt gccgcctctt caacggacat aggtttgatg tgatagcgtg
      181 aagacttgat cacgcgcggg ccttcctctg tcggttccaa gacgtcactg ccctcaagaa
      241 tgtctacttt gaaggactgc tgtaccatgt gctcagtgcg gagccgatga ttcttgatgc
      301 gatctttgta cttcttcagc tgtcgttcca tcttcttgat aaccagatca atggcggcct
      361 tcatatcctc ttcttcatct gtgctgtgaa gggtgatccc attggcctga atgctcattt
      421 cagcgtggaa acggcgcttc tcctgataca gcgtgacgtg tgcgacaaca attccttcaa
      481 aaaagtgctt gaggttcgcc agttttttct cagcgtagtc cttgacgtcc tctgggactt
      541 cgcagtgctt ggcagcgaag gaaacttgca tattctgcta cccctttcaa aacaataata
      601 atactcagcc caggcatgcc cgggcagcca atccaattct gtcactaccc tcaaaggtac
      661 caaaagcacc ggagaggtgt caacttgatc cgagatgcac acatttgcct tccctggcag
      721 cctctcttga cacggtctgg ccttgtgtgg cataggcaag acggtgtcct gcatcagaga
      781 cccgaatcgg tgaaacctgc aggaggttta tgtgatgctt gctattcttc gaaccatgct
      841 tccgatcgtt ttgctctgcc tttgtcaact cgctccctgt gccgttctga atgtgcctgc
      901 gcgccgtgga gacgcgatgt ccggcagcgc ctttgtgacc gcgatcacac ctttgtctct
      961 gacggagcgt gagaatgcca tcctgagcga agtgatgctg ggaaacgttc cggatttcat
     1021 gcgaaacctg aaacccgtca cggtctccgc gaccatcaac agccaactgc gcactgttat
     1081 ctaccatgtt ctcccagact atctggccgt tggatctgac gcggactatt ttctgacgcc
     1141 catgtcgccc ttgtgcgcac agcggattgc cgaccgcctc aattgccttc ttcccaccag
     1201 aaaaatggtg aacgacatct ggtcacaggc acaggtcaaa ctcagccctt ccaccatacc
     1261 gcccagtccg gagatgacca cggttcctgt cttcgcgcag cacaatacga cagttcgggc
     1321 acagagacag gcaatggaag tccagtatcc tctgggtgcg ttggtgggtg gtgacaaaaa
     1381 ggatgtcgtc atcaccaaac tgttggccac aactcccaac aaagtggcga tctacggctg
     1441 gcactacacg aatggaactc ccattcagcc tctctatctg gggcatgaag atacctacgc
     1501 ggactacagc cacggaatcc gactggttca acaggcagtg acactcgatg gcgttgaaac
     1561 cacggcggtt caaatcctca atgatgcgag cctgaatgtt ctgttgagcg atgaaggcgt
     1621 ggtgaccagc actcggtatc cggtggcatt gccgctgtat tttcccttgc aggacgattt
     1681 ccccgcgtcg ggtcgagatt ctctcctgtg gacagacaag ttcaagacgc cgacgctcat
     1741 gtcattctca cccacgtcgc cggggggaga cgggtatgcg ctccgagtga tggattccgc
     1801 gggcggtatg gagacgactc ggacgggaaa catcaccgat gcagactact atgttcagtg
     1861 cgacatctac tgcacctatc gccctgcgct cgtttcggac ggtttcgaac ggtcaggaat
     1921 cttccttcgc gacaatggaa atggtgcttt tgagcataca accggcggtg gtggttattg
     1981 ctacgcgatg gcttgggatt caggcaacgg acgtctctgg tgtctgaaat ccgtcaacgg
     2041 ggtgatcacc
//
LOCUS       DAOQUC010000766         2050 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_69683_length_2050_cov_2.432581, whole genome shotgun sequence.
ACCESSION   DAOQUC010000766 DAOQUC010000000
VERSION     DAOQUC010000766.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69683_length_2050_cov_2.432581"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..426
                     /locus_tag="PKH07_12405"
     CDS             <1..426
                     /locus_tag="PKH07_12405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35784.1"
                     /translation="DASNANFASTMVAESPAVREFEDWQAFFGVFFQEIYRRVIESGV
                     RSGQLPEGSVQTEVHDGQESEQFKPTSLEVHLQFPPLVHREILQDARAYEIYLKSRIA
                     SRRSIAARAGFNFDIETEHMLREQDVQQSERDVTSESRQ"
     gene            complement(493..813)
                     /locus_tag="PKH07_12410"
     CDS             complement(493..813)
                     /locus_tag="PKH07_12410"
                     /inference="COORDINATES: protein motif:HMM:NF014633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HNT35785.1"
                     /translation="MSNTENEPQWCSSCSEGTVLDIRVAPKCSNARVVGEHGMQLKIS
                     LCSAPEGGAANEELIRFLAKRLGISKSRVILQSGFKSRSKRILLTDTQMTERKVREAL
                     LPDG"
     gene            complement(800..>2050)
                     /gene="ptsP"
                     /locus_tag="PKH07_12415"
     CDS             complement(800..>2050)
                     /gene="ptsP"
                     /locus_tag="PKH07_12415"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HNT35786.1"
                     /translation="QMRKEKVIAFVTDVGGPTSHTAIMARSLEIPAVVGLQDITAHVQ
                     SGDRLIVDGVEGIVIVNPSESVLRKYQTKQQEAKGKLADLVHLRDLEAETLDGFKVSL
                     GANIEMQDEVDAAVRYGAEGVGLFRTEFLFLNQVNLPGEEQQFEAYRKVAEVAAPHPV
                     VIRTLDVGGDKFVSSPEFPTGMGDSLGCRAIRFSLTHPDLFRTQLRAILRASAYGNVR
                     IMYPMITSVEEVLRSNEMLRECMDTLRTEGMPFNEDIEIGTMIEVPSAALMADHLAQE
                     ADFFSIGTNDLIQYTLAVERTNQDMAYLYDPLHPAILRFIHHTVQTAHEAGIPVGVCG
                     EMAGEPAVIPLLLGLGVDSLSMSPISIPMAKKIIRELELSPLRKWVQGILNLPTGQEV
                     RNQIKAYELEFAKKDSKPKSHVKH"
BASE COUNT          450 a          562 c          555 g          483 t
ORIGIN      
        1 gacgcaagca acgccaactt cgccagcacg atggttgccg aaagcccagc tgttcgggaa
       61 ttcgaagact ggcaggcgtt tttcggggtg ttttttcagg agatctatcg gcgtgtgatt
      121 gaatctgggg tacgttcagg acaactgccc gaaggcagtg tgcagacaga ggttcatgac
      181 gggcaagagt ctgagcagtt caaacccacg agtctcgaag tgcaccttca gttcccacct
      241 ctggttcatc gggaaatact ccaggatgcc cgagcttatg aaatctatct caagagccga
      301 atcgcctcac gccgttccat cgccgcgcgc gccggcttca atttcgacat cgaaacagaa
      361 cacatgttgc gagagcaaga cgtacaacag tctgagcgtg atgtgacctc cgaatcgcga
      421 caatgactga cagcacacaa caagtgagcc atccgtgcac ttgcttcttt gttgccgttg
      481 aaaggtggga cttcagccgt ctggcagcaa cgcttctctg acttttctct ctgtcatttg
      541 cgtgtcggtg agaagtatcc gcttggaacg tgacttgaag ccggactgca gaattacccg
      601 actcttgctg attccaagtc gttttgccaa aaaccgtatg agttcctcgt ttgctgcccc
      661 gccttctgga gccgagcaga gggagatttt caactgcatt ccgtgttctc cgaccactcg
      721 cgcgttggag cacttgggag caacgcgaat atcgagcaca gtcccctcag aacaactcga
      781 gcaccattgg ggttcattct cagtgtttga catgggactt gggtttggaa tccttcttgg
      841 cgaattccag ttcgtaggct ttgatctgat ttcgaacttc ctgacctgtg ggaagattca
      901 ggattccctg cacccatttg cggagaggtg agagttcgag ctcccggatg atcttctttg
      961 ccatgggtat ggagattgga ctcatgctga ggctgtccac ccccaaaccc agaagcagcg
     1021 ggattaccgc tggttccccg gccatctctc cgcagacccc aacagggatg ccggcctcat
     1081 gcgccgtctg aaccgtgtga tggatgaagc gaagaatcgc gggatgcaga ggatcgtaga
     1141 gataagccat gtcctggttg gttctttcaa cggcgagagt atactggatc aagtcgttgg
     1201 tgccgatgct gaaaaagtct gcttcctgcg ccagatgatc ggccatgagc gccgcggagg
     1261 gcacttcgat catggttccg atctcgatat cctcattgaa cggcattccc tcggtgcgaa
     1321 gagtgtccat gcattcacgc agcatctcat tcgatcggag aacctcttcg accgatgtga
     1381 tcatcggata catgatccgg acattgccat aggcgcttgc gcgaagaatc gcgcgaagtt
     1441 gcgttcggaa aagatcgggg tgggtcagac tgaaacgaat cgcccgacaa ccgaggctgt
     1501 ctcccatgcc cgttgggaat tccggggaac tgacaaactt gtctcccccc acgtccaaag
     1561 tccggatcac aacgggatga ggcgctgcaa cctccgccac ttttcggtac gcctcgaact
     1621 gctgttcctc gccgggcagg ttgacctggt tcaggaaaag gaactcggtt cggaacaagc
     1681 cgacaccttc cgcgccgtat cgaaccgctg cgtccacttc atcctgcatc tcaatgttgg
     1741 ctccgaggga gaccttgaat ccatcgagtg tttcagcttc caaatccctc agatgaacca
     1801 agtcggcaag tttgcccttg gcttcctgtt gtttggtctg gtatttccga agaacggatt
     1861 ccgacgggtt gacaatgaca attccttcca ctccgtctac gatgagacga tctccggact
     1921 gaacatgggc ggtgatgtct tgaagaccga ccaccgcggg gatttccaga gatcgcgcca
     1981 tgatggcagt atgcgaggtt gggccaccga cgtcggtcac aaaggcaatg accttttcct
     2041 tgcgcatctg
//
LOCUS       DAOQUC010000767         2049 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_69725_length_2049_cov_2.954363, whole genome shotgun sequence.
ACCESSION   DAOQUC010000767 DAOQUC010000000
VERSION     DAOQUC010000767.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69725_length_2049_cov_2.954363"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="PKH07_12420"
     CDS             complement(<1..228)
                     /locus_tag="PKH07_12420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35787.1"
                     /translation="MANETPLSDIRDNPIRVIVFDHTPELREAVATVLRRRGYDVHAC
                     ERGREALALMDQHEFEVMVTNAATAQTSSSDF"
     gene            complement(228..1289)
                     /locus_tag="PKH07_12425"
     CDS             complement(228..1289)
                     /locus_tag="PKH07_12425"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNT35788.1"
                     /translation="MVECLMGDVLNHLEREETRETGRPRILVVDDEEIMLSMLVEHLQ
                     GQSYEVDGCYNGAEAMKRIDQYEYAVLITDLVMTPIQGQEIVAYLKKHHPDTEAIVMT
                     AYASVDRAVEAIHQHVFDFVEKPFSLRRIDVTVSNAISKYRLSLENRKLVEELARQNE
                     RLEERIRNATQDLQDLTVKDGLTGLNNYRFYQSIIVAEISRCIRYGRDLSLAVLDLDF
                     FKIYNDQHGHQAGNEALCLLAQILESNSRESDHIIRYGGEEFVVVFPETGKKEAAKNV
                     ERTSEALRKASLTVDSPTGLGFLTLSAGLATCPADATEAESLMRAADMALYDAKNQGR
                     NRIVLADESHQKARQGTSE"
     gene            complement(1403..>2049)
                     /locus_tag="PKH07_12430"
     CDS             complement(1403..>2049)
                     /locus_tag="PKH07_12430"
                     /inference="COORDINATES: protein motif:HMM:NF025953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HNT35789.1"
                     /translation="SENQFGEWLASADAVWELIPRISDLIQATFIPGILGEVSPTAQF
                     GENVFIGEDAKVLAGTVILGPAIIGRGCTIGPNAYIRQNVIICDGAHVGHVCEVKNAL
                     LMEGALVPHFAYVGDSLLGRKAHLGAGVKLSNVRIDGKSVTVKIGGERHETGLRKFGA
                     ILGDEAEVGCNAVLNPGTLVGPRTLISGALLAGGCYPQDSFVSHGHTPEVRPKK"
BASE COUNT          464 a          583 c          514 g          488 t
ORIGIN      
        1 aaagtccgaa ctggacgtct gagcggttgc ggcattggtc accatgacct caaactcatg
       61 ttgatccatc aaggccaggg cttcccgtcc ccgttcgcag gcatggacat cgtagccacg
      121 ccttctgagc acagtggcca ctgcctcacg aagttctgga gtatggtcga aaacaatcac
      181 acggatcgga ttgtccctta tgtcacttag tggggtctcg tttgccatca ctcagacgtt
      241 ccttggcggg ctttctggtg agactcatca gccagaacaa ttcggttgcg tccctgattc
      301 ttggcatcat agagagccat atccgcggcg cgcatcaggc tttccgcttc agtggcgtct
      361 gccggacagg tggcgagccc agcactcagg gtcaggaatc caagtcccgt aggagagtca
      421 acggtcagtg atgctttgcg aagcgcctcg gatgtgcgct ccacgttctt ggccgcttcc
      481 tttttgcctg tttcgggaaa gacgacaacg aattcctcgc cgccgtaccg aatgatatgg
      541 tcggactcgc gtgagttgct ctccagaatc tgggcaagca gacagagcgc ttcattgccc
      601 gcctggtgac cgtgttggtc attgtaaatc ttgaagaaat ccaaatccaa aacggcaaga
      661 gaaagatcgc gaccgtagcg aatgcagcga ctgatctccg ctacgatgat actctgatag
      721 aaccgatagt tattcagacc cgtaagacca tctttgactg tcaaatcctg taggtcctgg
      781 gtcgcattgc gaattcgttc ctccagccgt tcgttctgtc tggcaagttc ctcaaccagt
      841 ttgcggtttt ccagcgacaa ccgatacttg gagatggcgt tgctgacagt gacatctatc
      901 cggcgaagac tgaagggttt ctccacaaag tcgaacacat gctgatgaat tgcttccaca
      961 gcccgatcga ccgaagcgta agccgtcatg acgatggctt cggtgtccgg atgatgcttt
     1021 ttgagatagg caacgatctc ctgtccctga atcggagtca tgacaaggtc ggtgatcaga
     1081 acggcgtact cgtactgatc tatacgcttc atggcctctg ccccgttata gcagccgtcc
     1141 acttcgtagc tttggccttg aagatgctcc accagcatgg acagcatgat ctcctcgtca
     1201 tccacaacga ggattcgggg cctgccggtt tctcttgttt cttctcgttc caaatgattc
     1261 aggacatcgc ccatgaggca ttcaaccatc ctaaaggaag tcctaaccga acacttagta
     1321 aacgaatata ctttggtttt cccgaaaagt caaaaagcat gcgtgtcatt ctctctcacg
     1381 cgagggaaag ccaagggtct gtctattttt ttggccgaac ttcaggggta tgtccatggc
     1441 tcacaaaact gtcctgggga tagcatcctc cggcgagcag ggcacccgaa atgagcgttc
     1501 ttggcccgac cagtgtgccg gggttcaaca cggcattaca gccgacctct gcctcgtcgc
     1561 ccagaatggc accgaacttg cgcaggccgg tttcatgacg ttctccgcca atcttgaccg
     1621 tcaccgactt cccatcaatt cgaacattgg acagtttgac gcctgcgcca agatgcgcct
     1681 ttctgccaag cagactgtct cctacatacg cgaaatgcgg cactaacgca ccttccatca
     1741 atagggcgtt cttgacttcg caaacatgac ccacatgggc accgtcgcag ataatgacat
     1801 tctgacggat gtaggcgtta ggacctatag tgcatccccg cccaatgatc gcgggaccca
     1861 ggatcaccgt tccagcaaga accttggcgt cttctccgat aaagacgttc tcgccgaact
     1921 gtgcggtagg actaacctca cccaggattc cagggatgaa ggtcgcctga atcaggtcgc
     1981 tgatccgggg aatcagttcc caaacggcat cagcactcgc aagccattct ccgaactggt
     2041 tttccgaca
//
LOCUS       DAOQUC010000768         2048 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_69762_length_2048_cov_3.512293, whole genome shotgun sequence.
ACCESSION   DAOQUC010000768 DAOQUC010000000
VERSION     DAOQUC010000768.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69762_length_2048_cov_3.512293"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1649
                     /locus_tag="PKH07_12435"
     CDS             <1..1649
                     /locus_tag="PKH07_12435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35790.1"
                     /translation="VADRKSLTLVALEPRGIYYASKTVQQLLKAKRVGDRVGIPMMEV
                     TDWPDLEDRGLWGCDAYHHMRWLSDRKMNYMEQIASPRVDENKRPIAWLSEAKMQMVT
                     EGPTYGINPVPAVLHLEQLGGGGLFDQYPELVAKKTGEREKSPICYSQPVFVDILADW
                     LVGYAQMPGVTEVDVWMAENLAQKGGCECDECSKHDRNVLEAQVIVAAYEKAKKRVPS
                     LRIRTLTSEETENSNIEVFKVFPPEVKIWYYHSLLTYTAGETALLWRPYLEDFAKGGR
                     WLGVCPSLFAAGHFTHPFTAAQFVHYRLNEFVDKGLKGLIGYAIPRTYYLSFNVEAAM
                     EWAWNAKGRTPREFAYSWAVREGLPDPNLFAEWSETLGPVAWDVYGSDFPVGEQRNTP
                     GKVAKRLRDGTLKELGFVLWDAYRSPWGDIKSEAQLEQDSILADKAVQLARQMGREEY
                     IHESLIAQGYIRSLKALWELKQIVEPSEIAEKDRAAAKTYFRMYIDGLRQASDSLPKW
                     EATLPLRKQDEDFTEKPVGVIAEMIKEMQQVASDFGILLQ"
     gene            complement(1658..>2048)
                     /locus_tag="PKH07_12440"
     CDS             complement(1658..>2048)
                     /locus_tag="PKH07_12440"
                     /inference="COORDINATES: protein motif:HMM:NF024498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNT35791.1"
                     /translation="SLPSLDSQTVTLSQFKGKPVMLAFLASWSGSCKRQAPIVETVFQ
                     ASKSQDLVVIGIFSERAEDKIKEFATTQGLTFPIVVDAEKSVMKDFNVNSWPCFVLLD
                     KEGSVKKELPGMRTSDELREALGTLGQ"
BASE COUNT          492 a          517 c          592 g          447 t
ORIGIN      
        1 ttgttgctga caggaaatca ttgacgctgg tggctctcga accacgcggc atctattacg
       61 catccaagac agttcagcaa ctgctcaagg ccaagcgcgt tggtgacaga gtcggcatcc
      121 cgatgatgga agtgaccgac tggcctgacc ttgaagatcg agggttgtgg ggttgtgacg
      181 cctatcatca catgcggtgg ctttccgacc gcaagatgaa ctacatggaa cagattgcca
      241 gtccccgggt ggatgagaac aagcgtccca ttgcctggct ttccgaagcc aagatgcaga
      301 tggtgacgga aggcccaacg tacggaatca atcccgtgcc cgcggtactt catctggagc
      361 agctcggtgg agggggactc tttgaccagt atccggagct tgtcgccaag aagacgggag
      421 aacgagaaaa gagcccaatc tgctactcgc aacctgtgtt tgtggatatt ctcgcggact
      481 ggcttgtcgg ctatgcgcag atgcccgggg tgactgaggt ggatgtctgg atggcggaaa
      541 acctcgcgca gaagggcggc tgtgaatgtg acgaatgcag caaacacgac cgaaatgtgc
      601 tggaagcaca ggtgatcgtt gcggcctatg agaaagccaa gaaacgggtt ccaagccttc
      661 ggatcagaac actgacaagc gaggaaacgg aaaacagcaa catcgaggtc ttcaaggtgt
      721 ttccgcctga agtcaagatt tggtactacc acagtctgct tacgtatact gcgggagaga
      781 ctgctctgct ctggagaccc tatctggaag attttgccaa agggggacga tggctcggcg
      841 tctgtccgag cctctttgca gccgggcatt tcacgcatcc tttcaccgcg gcacagtttg
      901 tccactatcg cctgaatgaa tttgtggaca aagggctcaa gggtctgatc ggctacgcaa
      961 ttccacgaac gtattatctg tcgttcaatg tggaagcggc gatggaatgg gcatggaacg
     1021 cgaagggcag aactcctcgt gagttcgctt acagctgggc ggttcgcgag ggactgcccg
     1081 atccgaatct tttcgccgag tggtcagaaa ctttggggcc ggtggcatgg gatgtatacg
     1141 gttcagattt cccagttgga gagcagcgca ataccccagg caaagttgcc aaacgcctca
     1201 gagatgggac gttgaaagaa cttggttttg tgctctggga tgcctatcgc tctccttggg
     1261 gtgacatcaa atccgaagcg cagttggagc aggattccat cctggcggac aaagcggttc
     1321 agttggctcg tcagatggga cgcgaagagt acatccatga gagtctgatt gcccagggat
     1381 atatccgttc gctcaaggca ctctgggaac tcaagcaaat tgtcgagcca tcggaaatcg
     1441 cagagaaaga ccgagccgct gcaaagacgt atttccggat gtacattgac ggactacgtc
     1501 aggcttccga cagtctgcca aagtgggaag caacgctgcc tcttcgcaag caggacgaag
     1561 acttcaccga gaaaccggtc ggagtcatcg ccgagatgat caaagagatg caacaggtcg
     1621 catccgattt tggtatcctg cttcagtaga gcaaccgtca ctgtccgagt gtgccaagtg
     1681 cctcgcggag ttcatcactg gtgcgcattc caggaagttc tttcttgaca ctgccttctt
     1741 tgtcaagcaa gacaaagcac ggccatgagt tcacgttgaa atccttcatg acactcttct
     1801 cggcatccac aacgatgggg aatgtcagtc cctgggttgt ggcaaactcc ttgatcttgt
     1861 cttccgcccg ctcactgaat atgccgatga ccaccagatc ttggctcttg gatgcctgaa
     1921 agacagtttc cacgattggg gcttggcgct tgcacgaacc actccaggaa gccagaaacg
     1981 caagcatcac cggtttgcct ttgaactgag aaagggtgac cgtctgactg tccaacgagg
     2041 gcaggctg
//
LOCUS       DAOQUC010000769         2048 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_69786_length_2048_cov_2.724034, whole genome shotgun sequence.
ACCESSION   DAOQUC010000769 DAOQUC010000000
VERSION     DAOQUC010000769.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69786_length_2048_cov_2.724034"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..709
                     /locus_tag="PKH07_12445"
     CDS             <1..709
                     /locus_tag="PKH07_12445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35792.1"
                     /translation="KRSSPLLWLDGIELDGPDYAGSYYHIVCLGRFTGITREMGFTQA
                     LMEARKQGGLLILAHPDWMGNTFEEAARYGVHGVEVYNHVCRWLNGKGDGWPYWHSLL
                     DRHPATLGLAVDDAHIRSEHPGWNGGWIQVDSPELNSEGILEAIRAGRFYSSCGPEIH
                     SLEFDGKDLHVKTSPVQFVRLVGPSWRGQRVGSFNGDVRTETSLSVPLDWPYVYLEIE
                     DSSGRRAWTNALFVSEI"
     gene            853..2025
                     /locus_tag="PKH07_12450"
     CDS             853..2025
                     /locus_tag="PKH07_12450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNT35793.1"
                     /translation="MQQTPRSQQRHDGLDSMVPFEMMSPSARKLRDIYALKPGAPLLH
                     REFGYYCLEEWKKQGMPEDVPLEQLFHYDPPGRFDLQELGWTEAAFRPHFEEKVIEDR
                     GDHELVQDRSGRHVLFFKGRRSGFMPTYVANPAKDWKTWEENCKWRLDPNTPVRLETL
                     PQRMQQAQAKAAEGLLIGQRIIGGYMYLRSLFGPEELVYLFYDAPDLLQDCMQTWFHL
                     ADTVIARHQEYVTLDELFLSEDICYNHGPLISPDMMRDFLIPYYQQLIGNIRSRQRDK
                     SRQLHIQVDTDGYAVPVIPIYQGIGMDVMSPFEVASGCDVVEIGKQFPNLVILGGIDK
                     RVLAESKEAIDRMVERIIPPMKARGGYIPTCDHGVPAEVPYENYLHYRKRCVELGG"
BASE COUNT          442 a          527 c          593 g          486 t
ORIGIN      
        1 caagcgttcg agccctctgc tttggctgga cggcattgaa ctggatggcc ctgactacgc
       61 gggctcctac tatcatattg tctgtcttgg cagattcaca ggaatcacgc gtgagatggg
      121 ctttacccag gcgcttatgg aagcgcgcaa gcagggaggc ctgttgatcc tggcgcatcc
      181 cgactggatg gggaacacat ttgaagaagc cgcccgctat ggggtgcatg gcgtggaagt
      241 ctacaaccac gtctgccgct ggctcaatgg taagggggac ggttggccgt attggcactc
      301 cttgctggat cgccaccctg cgacactcgg cctcgcagtt gatgatgccc atatccgttc
      361 cgagcatcct ggctggaacg ggggatggat tcaggtggac agtcctgagc tgaactccga
      421 agggattctc gaagcgattc gagccggacg cttctattcc agttgtggac ccgagattca
      481 ttcgctggaa ttcgatggca aggatcttca tgtgaagaca tctcctgttc agtttgtcag
      541 actggttggg ccctcttggc gcggccagag agttggatca ttcaacggag acgtgagaac
      601 cgaaacaagt ctttcggtgc cgttggactg gccctatgtg tatctggaaa tcgaagattc
      661 ctctggcaga cgcgcatgga caaacgcgct gttcgtgtct gagatctaga tttccttctt
      721 aggcaggtta gcacctgcgg gcgcgtgggc tatgtccgct ccttcggagc ttgcccaata
      781 aatgacgcgc cgctctcgga ctttgtgtca ccagtaggat ggccttgtac aacaaaagag
      841 gatggtttct tcatgcagca gactccccgt tcacagcaac gacacgacgg tcttgacagt
      901 atggtgccct tcgagatgat gagcccaagt gccaggaaac tccgagacat ttatgcgctg
      961 aagccgggtg cgcccctgtt acatcgggag ttcggttatt actgtttgga ggagtggaaa
     1021 aagcagggga tgccagaaga cgttcctctg gagcagttgt ttcactatga tccgccagga
     1081 cgttttgacc ttcaggaact cggctggacg gaagccgcct tccgtcccca ctttgaggaa
     1141 aaagttatcg aggacagggg tgatcatgaa ctggttcagg atcgctctgg acgtcatgtg
     1201 cttttcttca agggcaggcg ttccggtttc atgccgacct atgttgccaa tccggccaag
     1261 gactggaaga cctgggagga aaactgcaag tggcgtcttg acccgaacac gccggttcgg
     1321 cttgagacac tgccacagcg aatgcagcag gcgcaagcaa aagccgcaga gggccttctg
     1381 atcggtcaga ggatcatcgg cggttacatg tatctcagga gtctttttgg ccctgaagag
     1441 ctcgtctacc tcttctatga tgcacccgac ctgctccaag actgcatgca gacatggttt
     1501 catcttgcgg ataccgtcat cgcgagacac caggagtatg tcacgctcga tgagctcttt
     1561 ctgtctgagg atatctgcta caatcacggc ccattgatct caccggacat gatgcgcgat
     1621 tttttgattc cttactatca acaactgatc ggcaacatac gctcccggca acgagacaag
     1681 agccgccagc tacatatcca ggttgacacg gatggatacg cggtaccggt aattcctatc
     1741 taccagggaa ttggtatgga cgtgatgtct ccttttgaag tggcctcggg ttgtgacgtg
     1801 gtggagattg gaaagcagtt tcccaacctg gtaatccttg gcggcattga caagcgtgtt
     1861 ttggcggaaa gcaaggaggc cattgaccga atggtcgaga ggattatccc accgatgaaa
     1921 gcgcgcgggg gttatatccc gacctgcgat cacggtgtgc ctgccgaagt gccttacgag
     1981 aactatcttc attaccgcaa gcgctgtgtc gaactcggtg gctgagcccg tccgtcggca
     2041 gtcggttt
//
LOCUS       DAOQUC010000770         2048 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_69794_length_2048_cov_2.329654, whole genome shotgun sequence.
ACCESSION   DAOQUC010000770 DAOQUC010000000
VERSION     DAOQUC010000770.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69794_length_2048_cov_2.329654"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..687
                     /locus_tag="PKH07_12455"
     CDS             <1..687
                     /locus_tag="PKH07_12455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HNT35794.1"
                     /translation="IVGFFATAGAAGVDFGTNSRDESDVNYGGIFGIALAIIFTGGAA
                     ILISAGGFGLTKIYALDPSNADFMSHIMGSQAIGKFMGLLLAIAAFPAACFSSFIAAN
                     SFKTTLPKVNPFVSVGIGALVSIILAVTGWAGQLLQVFTVIGASFGPICGAMVVDFWL
                     SGKKWAGPREGWNLAGWISWAVGFVVGMAPLVGLFSVPAAPLVAFIVGAVLYYVLAKA
                     GLESKTVSQR"
     gene            780..1730
                     /locus_tag="PKH07_12460"
     CDS             780..1730
                     /locus_tag="PKH07_12460"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015785359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNT35795.1"
                     /translation="MDLSVVIPVFNERENIIPLCEGLVTVLAGLGCSFEVIFVDDGST
                     DGTEAVLGEAVERWNGIRAIRFRRNFGQSPALQAGFDHAQGKVIVTMDGDLQNDPTDI
                     PRLLERLEEGYDIVSGFRSQRKEGVIRRLPSAIANWLLRKATGVPLHDTGCTLKAYRK
                     EVIESVRLYGEQHRFIPVLASQFGAKITEIPVKHWQRRAGKSKYGLSRTHRVFLDILM
                     LKFLLSYGTRPLQVFGVFGLGSFLLGIVINIYLTIKKIQGHPLAERPLLLLGILLVIL
                     GVQFVSMGLLAEMLVRTYHETSRRPTYWVERILRSKHEDA"
     gene            1809..1900
                     /locus_tag="PKH07_12465"
     tRNA            1809..1900
                     /locus_tag="PKH07_12465"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1844..1846,aa:Ser,seq:gga)
BASE COUNT          412 a          500 c          568 g          568 t
ORIGIN      
        1 atcgttggct ttttcgcaac tgccggtgct gcaggggttg attttgggac caacagcaga
       61 gacgagtctg atgtgaacta cggcggcata ttcggaattg ctctggcaat catcttcacc
      121 ggtggcgctg ccattctgat tagtgccggt gggttcgggt tgacgaagat ttatgcgctt
      181 gacccgtcga acgctgactt tatgtctcac atcatgggat cccaggcgat cggtaagttt
      241 atgggattgc ttctggctat tgctgccttc ccagctgcgt gcttcagctc gttcatcgct
      301 gcaaatagtt tcaagacaac tttgccgaag gtcaatccct ttgtctcagt tggaattggc
      361 gctctcgtga gcatcatcct cgctgtgacc ggatgggcag gtcagctgtt acaagtgttc
      421 acggttatcg gagcttcttt tggtccaatt tgtggcgcca tggtcgtaga tttctggctc
      481 agcggcaaga aatgggcggg accgcgtgag ggatggaacc ttgccggttg gatttcctgg
      541 gcggttggtt ttgtcgtcgg tatggctcct ttggtcggac tctttagcgt gcctgccgct
      601 ccgcttgtcg cgtttatcgt tggcgcagtg ctttattacg ttctggcaaa ggcagggttg
      661 gagtccaaga ctgtctctca acgatgatag gaatcacaag agtttcctgt ctttcccctg
      721 tgggtgtcct ttcgccacaa acgagagcat tgtgcttggc tcaggagcct ttttgaacga
      781 tggatctttc cgttgtaatt cccgttttca atgagcggga gaacattatc cctctctgcg
      841 agggtttggt gactgtgctt gcaggtctgg gctgttcgtt tgaagtcatc tttgttgatg
      901 atggcagcac agacggcaca gaggcagtac ttggtgaggc tgtcgagcgt tggaacggca
      961 ttcgggcaat ccgatttcgt cgcaactttg gacagagtcc cgcacttcag gcaggttttg
     1021 atcatgctca gggaaaggtc atcgttacaa tggacggtga tcttcaaaat gatccgacag
     1081 acattccccg actcttggaa cgtctcgaag aaggctatga cattgtgagc ggattccgct
     1141 cacagcgcaa agaaggcgtt atcaggcgac ttccttccgc catcgcaaac tggctcctgc
     1201 gcaaggcaac cggagtccct ctgcatgaca cgggttgtac gctgaaagcc tatcgaaaag
     1261 aagtgatcga atctgtccga ttgtacggcg aacagcaccg ctttattcct gttctggcga
     1321 gccagttcgg ggcgaagatt accgaaattc cagtcaagca ctggcaaaga agagcaggga
     1381 agagcaagta cggtctttcc agaacacacc gggtttttct cgacattctg atgctaaaat
     1441 tccttctcag ctatgggaca agaccgctgc aggtcttcgg tgtgttcggg ctgggctcct
     1501 ttctgttggg catagtcatc aacatttatc tgaccatcaa gaagattcag gggcatccat
     1561 tggcagagcg accccttctg cttcttggga ttctcctggt gattctgggt gttcagtttg
     1621 tctccatggg gcttctggcg gaaatgcttg tcagaacgta ccatgagacg tcgcgtcgtc
     1681 caacctattg ggtggagcgg attcttcgat ccaaacacga ggacgcctag agcccttttg
     1741 tggcaacgac tcaatctggg gttgaccgtt ggcacctgtc acgttatatt cctctgtctt
     1801 atctgcttgg agaggtgacc gagctggtcg aaggtgcacg attggaaatc gtgtgtgggc
     1861 caaaagctca ccaagggttc gaatcccttc ctctccgcca cctatttctt cagtgcatta
     1921 tagaccgcct taagagaact gtctcccatc gtcttggttg gagtgagata gcccccttcg
     1981 ccgttttcgt tccaggcgta cagaagcagc aatcgttctt cagtgatatt gtcagggtga
     2041 gcatccat
//
LOCUS       DAOQUC010000771         2047 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_69828_length_2047_cov_3.222390, whole genome shotgun sequence.
ACCESSION   DAOQUC010000771 DAOQUC010000000
VERSION     DAOQUC010000771.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2047
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69828_length_2047_cov_3.222390"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            171..1370
                     /locus_tag="PKH07_12470"
     CDS             171..1370
                     /locus_tag="PKH07_12470"
                     /inference="COORDINATES: protein motif:HMM:NF021391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2092 domain-containing protein"
                     /protein_id="HNT35796.1"
                     /translation="MDNRKSEEEMQGMLKQTRDTPVPDEVKARLQAHLTAFRRKMDAA
                     EAARFRPVFRLWSWRSVAAVASFAAILLLFLFSNPLRKGNSGISFADVLEQLKTFRPY
                     SWTDSIQYENKPALKMRVMKPSLSVRREIREDGSIYVFDLQAGKTLQLLPEKKYARLT
                     VYTEDEPKQDPDLLAMIGQLQDGTQQELGKRKIDGTMATGFKVPGGMTTWTVWVDPET
                     CLPVRVELDQETKPRRKIVMSDFDFAPAFDESLFNMEPPEGYAVEKIVRKRANPTERS
                     FIEGLRFLSEFLGGRFPSSLEGSELAKELADRVKELGGSRSNEETDKINEYHSEVTRY
                     VQILKGFTKVENFSYVGGTVTPGDKSAPILWYREKGSKTYRVIYGDLSACQRSSEELP
                     DSTSRNN"
     gene            complement(1527..>2047)
                     /locus_tag="PKH07_12475"
     CDS             complement(1527..>2047)
                     /locus_tag="PKH07_12475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35797.1"
                     /translation="NTDWEAQIENRPEEIREDHSLNLFPFLWSGEKHVWKADSKAKKE
                     LTDHERHHWVLPLYTFNEDSSMKVFTMLWPLWLQTDSEDEDSYAVLLKIAEASFRNNG
                     DRTVSVLWRGYRDEIRGEQRKISIFPFISHKRTAPDNWRTKFLEGFFEIGAESGDNYF
                     RILYLPRMGLDQ"
BASE COUNT          475 a          554 c          553 g          465 t
ORIGIN      
        1 agcacaagcc gtgcttatga gattcgtcga ggatctgtcc tacggcgata ttgcgcaggc
       61 cttgggctgc gccgagacga cggttcgatc tcatattgcc aaagcccgca aacagttagc
      121 cctcactatc gagaatctgt cttgaggcgg gagttaagga gttaccaact atggacaata
      181 gaaagagtga agaagaaatg cagggaatgc tcaaacaaac tcgggacacg ccagtgcccg
      241 atgaagtgaa agcccgtctg caggcccatc tgacggcctt ccgacggaag atggacgccg
      301 ccgaagcagc ccgtttccga cctgtctttc gcctctggtc atggcgctct gtggcagcgg
      361 ttgcctcatt cgcggccatt ctgttgctgt ttttgttcag caatccgctg agaaaaggca
      421 attccggtat cagttttgca gacgtcttgg agcagttgaa gacgttccga ccttacagtt
      481 ggacggactc gatccagtac gagaacaaac ctgccttgaa gatgcgtgtc atgaaaccgt
      541 ctctttcggt tcgcagagag atccgtgagg atggaagcat ctacgtcttc gatctccagg
      601 cgggcaagac gctccaactg ctgcctgaga agaaatacgc tcgtttgact gtctacactg
      661 aggatgagcc gaaacaggat ccggatctgc tcgcgatgat cggtcagctg caggacggca
      721 cacaacagga acttggaaag cggaagatcg atggcacgat ggccacgggc ttcaaagtgc
      781 ccggcggcat gaccacatgg acggtctggg tcgatccgga aacgtgcctt cctgtgcgtg
      841 tcgaactcga ccaagagacc aaaccgcgac gcaagatcgt catgagcgac tttgacttcg
      901 ctcccgcgtt cgatgaatcc ctcttcaaca tggaaccccc ggaaggctat gctgttgaga
      961 agatcgtccg aaagcgagca aatcccacgg aacgcagttt catcgagggt ctgcggttcc
     1021 tatcggagtt cctgggtgga cggttcccgt catccttgga gggcagtgag ttggcgaaag
     1081 aactcgcaga ccgagtcaag gaattggggg gctccagatc gaacgaggaa accgacaaga
     1141 tcaacgaata ccattccgaa gtgacgcggt acgtccaaat cctgaaaggt tttacgaaag
     1201 tcgagaactt ctcttacgtt ggcggcaccg taacgcctgg agacaaatcg gcgccgatcc
     1261 tgtggtacag agagaagggc tccaagacct acagagtcat ttacggtgat ctgagtgcct
     1321 gtcaacggtc ttccgaggaa ctcccagatt cgacctcgag aaacaactga atgtccttgg
     1381 ggtatccccc gcctcatgcg ttctcacttg gggcgggggg cactctaccc tcacctcgac
     1441 aaatctccca atcgctttcg tctgggctcc acccctcacc cgcagttgca cgaatccgcc
     1501 tgacggtcta atgcttcgct gacaatctac tgatccaatc ccatcctggg cagatacagg
     1561 atgcggaagt agttgtcgcc ggactctgct ccaatttcga aaaagccctc aaggaacttt
     1621 gtccgccaat tgtctggcgc agtacgtttg tgactgatga acggaaagat gctgattttg
     1681 cgttgttctc ccctgatctc gtcacggtac cctcgccaga gtacgctgac agtgcgatcc
     1741 ccgttattgc ggaaagacgc ttcggcgatt ttcagaagca cggcgtaaga gtcctcatcc
     1801 tctgagtccg tctgcaacca gagaggccag agcatagtga agaccttcat gctgctgtct
     1861 tcattgaatg tgtaaagggg cagaacccaa tgatgtcttt cgtggtctgt cagttctttc
     1921 tttgccttgc tgtccgcctt ccagacgtgt ttctctccgc tccagaggaa tgggaagaga
     1981 ttcagcgaat gatcttctcg aatctcctca ggacggtttt caatctgggc ttcccagtct
     2041 gtgttgc
//
LOCUS       DAOQUC010000772         2045 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_69918_length_2045_cov_3.758291, whole genome shotgun sequence.
ACCESSION   DAOQUC010000772 DAOQUC010000000
VERSION     DAOQUC010000772.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69918_length_2045_cov_3.758291"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..665
                     /locus_tag="PKH07_12480"
     CDS             <1..665
                     /locus_tag="PKH07_12480"
                     /inference="COORDINATES: protein motif:HMM:NF013502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CheB methylesterase domain-containing protein"
                     /protein_id="HNT35798.1"
                     /translation="TAQTLEPATSSAQKTSLSSVRDRMTHLAGKRPELVAIGISTGGP
                     NALQFLLAELPENLNLPVVIVQHMPPGFTGALASRLDESCALHVKEAQDLEPLMPNVV
                     YLAPGGRQMKVERIDDSIVLRITDDPAENHCKPSVDYLFRSVAESFPARAIGIIMTGM
                     GNDGALGTRTLRQTGCITIAQDMGSCVVFGMPKEAIQLGAIDIIVPLNKIAEEICKRV
                     FQ"
     gene            662..1504
                     /locus_tag="PKH07_12485"
     CDS             662..1504
                     /locus_tag="PKH07_12485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015905461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HNT35799.1"
                     /translation="MIKVSPDEYQVFASYIRSVSGIALDSSKTYLLETRLVDLLAQTG
                     SASFSELYYKIKTDSSLELTKKLVDAITTGETSFFRDGAPFELLQYKIIPDLVDKRKK
                     TTSPGLPIPIRIWSAACSKGQEVYSIAMTLKEILGDLSAYNIRILGTDISNKAVASAS
                     LGYFSQLEVERGLSPERIRRFLVKNESGWKISDEIRSLATFRRMNLLEDFSALGRFDI
                     VFCRNVAIYFNDDDRRKLFNNIGRVLEKDGYLLIGSTESILGFCPQFESKRYLRYVFY
                     QFKQ"
     gene            complement(1610..2005)
                     /locus_tag="PKH07_12490"
     CDS             complement(1610..2005)
                     /locus_tag="PKH07_12490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006065767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35800.1"
                     /translation="MYKIEKTGYGFRLTFAGAIPAEEMTKWVEESKTALAGRSGGFQL
                     LVDMRELKPLAPDAQQTMLQGQQLYKQAGMGRSAVILSSSILTMQFVRLAKESGIYQW
                     ERYLDASKNPNWEAQALAWLEKEQDPDKK"
BASE COUNT          493 a          571 c          492 g          489 t
ORIGIN      
        1 agacagcgca gacactcgaa cctgcaacca gctcagccca aaagacatcc ctctcctcag
       61 tccgcgacag aatgacgcat cttgcaggaa agagacccga actggttgca atagggattt
      121 ccactggagg tcccaacgct ctccagttcc tgctggcaga actccctgaa aacctcaacc
      181 tgcccgttgt gatcgttcaa cacatgcctc ccggcttcac aggtgccctg gcgtcgcgac
      241 tagacgaatc ctgtgctctg catgttaagg aagcccagga tctggagcct ctgatgccaa
      301 atgttgttta cctcgcccct ggaggcaggc agatgaaagt tgagaggatt gacgacagta
      361 tcgttctgcg catcacggat gaccctgcag aaaaccactg caaaccttca gtggactatc
      421 tctttcggtc cgtagccgag tcttttcccg cgagagcaat tggaatcatc atgaccggaa
      481 tgggaaatga tggagcactc ggcacaagaa ccctgcgaca gaccggctgc attaccattg
      541 cgcaagacat gggcagctgt gtcgtgttcg gtatgcccaa ggaagcgatt caactgggag
      601 ccattgatat catcgtccct ctcaacaaaa tcgctgagga gatctgcaag agggttttcc
      661 aatgatcaag gtgagccctg atgagtatca ggttttcgcc tcctacattc gctctgtcag
      721 cgggatcgct cttgattcga gtaagactta tcttttggaa acacgacttg tggatctcct
      781 ggctcaaaca ggctccgctt cattcagtga actgtattac aaaatcaaga cggactcctc
      841 tctggaactg accaaaaaac ttgtggatgc cataacaact ggcgagacct cgttcttccg
      901 cgatggcgca cctttcgaac tgctgcagta caagatcatc ccagacctcg tggacaagcg
      961 aaagaagacg acgtcccccg gtttgccaat cccgatccgc atctggagcg ctgcctgctc
     1021 caaggggcag gaagtctaca gcattgcgat gaccctcaag gagatactcg gtgacttgtc
     1081 cgcttacaac atacgcattc tgggaacaga catatcaaac aaagcggttg cctctgcaag
     1141 tcttgggtat ttcagccagt tggaggtgga aaggggattg tcgcccgagc gaatcaggcg
     1201 gtttcttgtc aagaacgaat ccggctggaa gatcagtgac gagattcgct ccctggccac
     1261 atttagaagg atgaaccttc tggaagactt ctccgcactc gggagattcg acatcgtctt
     1321 ttgtcgaaat gtcgcaattt acttcaatga tgacgaccga aggaagctgt tcaataacat
     1381 tggaagagtt ttggaaaaag atggctatct gctcatagga agcacagaat ccattctcgg
     1441 tttctgtcct cagtttgaat ccaaacgcta ccttcgttac gtcttttacc agttcaagca
     1501 gtagcagacc tcacgcttgg aacgaaaagg caaggagagc caagagcgct ctcctttcat
     1561 tctcgctgac cagtgccctg gagtctatac tccacagggc gcttgtccct catttcttgt
     1621 ctggatcctg ttccttctcc agccaagcca aagcctgagc ctcccagttg ggattctttg
     1681 aagcgtccag atagcgctcc cactgataaa tgccgctttc ctttgccagt cgcacgaact
     1741 gcattgtcag gatggagctg ctgaggatca ccgcggagcg acccatgcca gcctgtttgt
     1801 acaactgctg tccctggagc atggtctgct gtgcatccgg tgccagtggc ttgagttctc
     1861 tcatgtcaac caggagctgg aacccacctg atctacctgc caacgcagtc ttggactctt
     1921 caacccactt ggtcatctcc tccgctggaa tcgctccagc aaacgtcagc ctgaaaccgt
     1981 aacccgtctt ctcaatcttg tacatagtcc tactcctttc actttgtggc ctacgagagg
     2041 tctta
//
LOCUS       DAOQUC010000773         2039 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_70252_length_2039_cov_2.879536, whole genome shotgun sequence.
ACCESSION   DAOQUC010000773 DAOQUC010000000
VERSION     DAOQUC010000773.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70252_length_2039_cov_2.879536"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..661
                     /locus_tag="PKH07_12495"
     CDS             <1..661
                     /locus_tag="PKH07_12495"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNT35801.1"
                     /translation="QDIPIQMGYFPDVGIFGGLRWLLGDEECLIAFYTMPDLVHEIMD
                     HLTSLYLTVFEKVVREIRVDVIHIWEDMCGRQGPLISPAHWEEFISPNYRRIRAFADT
                     HGIPVISVDTDGNPDRITPPMIHSGVNLLFPLEVAAGCDINDLQARYPNLAFMGGIDK
                     RVLAEGHAAIDRELNRIKPAIVRGRYIPDLDHLIPSDVSWDNYCHYARSLKELVCSTG
                     E"
     gene            700..1551
                     /locus_tag="PKH07_12500"
     CDS             700..1551
                     /locus_tag="PKH07_12500"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020878155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HNT35802.1"
                     /translation="MDARTRLCAVIGKPIGHSLSPQIHNAAFAELGLNYSYVAFEVDD
                     VPGAMEGVRALGIAGLSVTIPHKVATMVEMDEVDPVAQDIGCVNTVVNSEGRLIGYNT
                     DGLGALHALLAAGCDLDGRSVLILGSGGAARAIAITLAHKCLLEKMTILGILEEERTI
                     LAQQAQSQTCYVVEHADLAESVLQEILPQHDVLIQCTPVGMYPKTEATLVPESLLRPD
                     LWVFDVVYNPLETMLLRQAKALGCHVVAGAEMFVRQAAAQFELWTGETAPINVMFDVV
                     LEALQRR"
     gene            1578..>2039
                     /locus_tag="PKH07_12505"
     CDS             1578..>2039
                     /locus_tag="PKH07_12505"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HNT35803.1"
                     /translation="MNVVLIGYRGTGKSTVAQIVARNLGWEALSLDQMIVQKAGKAIP
                     KIVDELGWEYFRDLESEVVVETAGRDKVVLDCGGGVILRAANVQALKDKGKVFLLTAT
                     PDTIVSRIKEDTNRPALIQGKTFLEEIAEVLESRNPLYRAAADVQIATDDKT"
BASE COUNT          466 a          463 c          589 g          521 t
ORIGIN      
        1 acaggatatc ccgatccaga tggggtactt tccggatgtg gggatttttg gcggtcttcg
       61 atggcttctt ggagacgagg agtgccttat agcgttttac accatgccgg atctggttca
      121 tgagatcatg gatcatctga cgtcgttgta tctgaccgtt ttcgagaaag tggtgcgtga
      181 gattcgagtt gatgtgattc atatctggga agatatgtgt ggtcggcagg ggccgctcat
      241 ctctcctgct cactgggaag agttcataag ccccaattac cgacgcattc gggcatttgc
      301 ggacacccat ggcatccctg tgatctcagt tgatacagac ggaaatccag atcgaatcac
      361 accgcccatg atccattctg gcgtaaatct tctctttcct ctggaagtcg cagcgggctg
      421 tgacatcaat gacctgcagg ctcgatatcc gaatcttgcc ttcatgggag gcattgacaa
      481 gcgggttctt gcagagggac atgctgcaat tgacagggaa ctgaatcgaa taaagcctgc
      541 catcgtacgt ggacgttaca ttcctgattt ggatcatctt attccatcgg acgtctcatg
      601 ggacaattat tgccactatg cacgttcgtt gaaggaacta gtctgttcga caggggagtg
      661 aatgtcgcca cgtcgcgtta gttttgaagg agagagttga tggacgcaag gacgagactg
      721 tgtgctgtta ttgggaagcc cattgggcat tccttgtcac cgcagattca caatgcggct
      781 tttgccgaac tgggattgaa ttactcctac gttgcctttg aagttgacga cgttccgggg
      841 gcaatggagg gggttcgtgc tttgggtatt gcgggtcttt ccgtcaccat tcctcacaaa
      901 gtggcaacta tggttgaaat ggatgaagtt gatcctgtcg ctcaagacat cgggtgcgtg
      961 aacactgttg tcaacagtga gggcagactc atcggataca acacggacgg tcttggagca
     1021 cttcatgcac ttctggcagc aggctgcgac cttgatggca gaagcgttct gattcttggc
     1081 tcgggcgggg cggcacgtgc tatcgccatc accctggctc acaaatgcct gcttgagaag
     1141 atgacgatct tggggattct tgaagaagaa cggacaattc ttgctcagca agcgcagtct
     1201 cagacatgct atgtcgtgga acacgccgac ttggcggagt ctgtattgca ggaaatcctt
     1261 ccccagcacg acgtgctgat tcagtgcact cctgtcggca tgtatccaaa gaccgaggca
     1321 actctggttc cagagtcact gctcaggccg gatttgtggg tcttcgatgt cgtatacaat
     1381 cctttggaaa cgatgcttct gagacaggca aaggcactgg gttgccacgt ggttgctgga
     1441 gccgagatgt ttgttcggca ggccgcggcg caatttgaac tatggactgg ggagacggcg
     1501 ccgatcaacg tcatgttcga tgtcgttttg gaggctctcc agagacgctg aagaaacaac
     1561 ctgtatgggg caagagtatg aatgtcgtcc ttattggcta tcgtggaaca ggcaaatcaa
     1621 cagtcgctca gattgttgcc cgcaatcttg ggtgggaagc gctcagtctt gatcagatga
     1681 ttgtgcagaa agcggggaaa gcaattccta agatcgttga tgagttgggt tgggagtact
     1741 ttcgtgatct ggagtctgaa gtggttgtgg agaccgccgg cagagacaag gtcgttttgg
     1801 actgcggcgg aggtgtgatc ttgcgtgcag caaacgttca ggcactgaag gacaagggta
     1861 aagtctttct cctgacggca actcccgata ctatcgtctc acgaatcaag gaagacacca
     1921 acagacctgc gcttattcag ggcaagacgt ttctcgaaga aattgctgaa gttctcgaga
     1981 gtcggaatcc attgtatcgt gcagctgcgg acgttcagat tgctacagac gacaagact
//
LOCUS       DAOQUC010000774         2039 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_70257_length_2039_cov_2.731351, whole genome shotgun sequence.
ACCESSION   DAOQUC010000774 DAOQUC010000000
VERSION     DAOQUC010000774.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70257_length_2039_cov_2.731351"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..871
                     /locus_tag="PKH07_12510"
     CDS             <1..871
                     /locus_tag="PKH07_12510"
                     /inference="COORDINATES: protein motif:HMM:NF013811.2"
                     /note="catalyzes the interconversion of 3-phosphoglycerate
                     and 2-phosphoglycerate; this enzyme does not require the
                     cofactor 2,3-bisphosphoglycerate as a phosphate donor;
                     BPG-independent PGAM; aPGAM; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HNT35804.1"
                     /translation="PRGFITDRRAGRISTEKCIELCQELSNKISIPGVEIHIKPEKEH
                     RAAVIFRGAGLSAGLSDSDPQHTGVPPLDVKALDATNEAAKSTAAMINEFIRKAKEVL
                     APHSPANMILLRGFDQYQKIPTMPELYHLQCAAIATYPMYKGVARLVGMDVIEGASNL
                     EDQIALLEKSWSDYTFFYFHVKYMDSRGEDGDFDGRIKVAEEFDAALPRIRALKPDVL
                     IITGDHSTPAKMKAHSFHPVPVCLCADLARKDDVNKFSEKAFLYGALGRIRGTDIIPL
                     ALAHAGKLVKYGA"
     gene            924..>2039
                     /locus_tag="PKH07_12515"
     CDS             924..>2039
                     /locus_tag="PKH07_12515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35805.1"
                     /translation="MVRNTRWKSTLGVVSIIAASLLVSLWIQVSWYVESGCLLQDVEQ
                     AIQGVSGTETTLGGIQIAKGPKVILSNLQVNYPPGQPLVSVGSLTASFRWHDLLFGRV
                     SRAVVEDVSLCLRFDDEGKLLFPSTDSQSSGKLVLPKIELINAAVSIHKKEIQTAFHV
                     SRLTFRHKIPMGYELIASLEVPHLADSLMLESSLPADFSLLKTKARVEIPRLNLSSLG
                     HYLATDLRLISDELPLGGNVSLSAELLTKGRLDVAARIEDASWGNFSGVNASLDLSTP
                     AFAEDESLQFTGSLGLLRLRLLEQPVLGEGDSSLTVDVKGTLERTQRGSNFAPEIQWR
                     LSPVRARVVERILLPFDLVKSGELGGQYMLAGTVHGST"
BASE COUNT          451 a          532 c          556 g          500 t
ORIGIN      
        1 accaagggga tttatcaccg accgacgggc tggaagaatt tcaacagaga aatgcattga
       61 actgtgccag gaactgtcga ataagatttc catccccggt gtagagattc acatcaaacc
      121 ggagaaggaa caccgcgccg cggtaatctt ccgaggagcc ggactttctg cggggctcag
      181 tgactcagat cctcagcata ccggagtacc acctcttgac gtcaaggcgt tggatgcgac
      241 caatgaggcg gccaagtcca ccgcagccat gatcaatgag ttcatccgaa aggcgaaaga
      301 agtgttggct ccgcacagtc cggcgaacat gatcttgctt cgaggatttg accagtatca
      361 gaaaatcccc accatgccgg aactctatca tctgcagtgt gcggcaatcg ccacttaccc
      421 aatgtacaaa ggtgttgcca gactggtcgg catggatgtc attgagggtg cttctaatct
      481 cgaagaccag attgcccttt tggagaagag ttggtctgac tacaccttct tctatttcca
      541 cgtcaagtac atggacagcc gtggggaaga tggagatttt gatggccgta tcaaagtggc
      601 tgaggagttt gatgcggcgc tgccaagaat cagggctcta aagcctgatg tgctgattat
      661 cacaggggat cactccactc ctgccaagat gaaggcacac agtttccatc cggtgcccgt
      721 gtgcctgtgc gcggatctgg ctcgcaagga cgatgtaaac aagttctcgg agaaggcctt
      781 tctctacggt gccttgggtc ggattcgcgg gacagacatc atcccgttgg ccttggcaca
      841 cgcaggcaag ctggtcaagt atggagcctg atcgcggcac ggttgcttga ccgaatgttt
      901 tcgactgcct tagaataggc gtcatggtga ggaatactcg ctggaaaagc acactgggcg
      961 ttgtgtccat tatcgcggcg tcgctgcttg tttccctgtg gatacaggtc agttggtatg
     1021 ttgaaagcgg gtgccttctt caggatgtcg agcaagcgat ccaaggtgtg tctggtacag
     1081 agaccactct cggtggcatt cagatcgcca aggggccgaa agtcatcctt tcgaacctcc
     1141 aagtcaacta tccgcccgga caacctttgg tctctgtagg ttcgctgacc gcctcgtttc
     1201 gctggcatga tctattgttc gggcgtgtct ctcgcgctgt cgtcgaagat gtttcactgt
     1261 gtcttcggtt tgatgacgaa gggaaactgc tctttccgag taccgactca caaagttcag
     1321 gcaaattggt ccttccgaag attgaactga tcaatgcggc ggtttcgatt cacaaaaagg
     1381 aaatccaaac cgcgttccat gtttcccgac tgacttttcg ccacaagatt cccatgggct
     1441 atgaactcat cgcctcgctg gaagttcctc atctggctga ctccctcatg ctggagtcct
     1501 ctcttccggc agatttctca ttgctcaaaa caaaggcgcg tgttgagatt cctcgtctga
     1561 atctctcttc tcttgggcac tatctggcaa ccgatctgag gctcatttcc gatgaactgc
     1621 ctttgggcgg aaacgtgtcg ttgtccgctg aacttctaac caagggacgc ttggatgtgg
     1681 cagctcgtat tgaagacgca tcgtggggca acttctctgg ggtgaacgct tcgctcgatc
     1741 tcagcacccc cgcctttgca gaggacgagt ccctgcaatt cacaggctcg ctgggtctgc
     1801 tccgtctgag actgctcgaa cagcctgtct tgggggaagg cgactccagt ttgaccgtag
     1861 atgtcaaggg gacgctcgaa cgcactcaga ggggcagcaa ctttgcgcca gagatacagt
     1921 ggcgtctgag tccagttcgt gcccgagtcg ttgaacgaat cctgcttcca ttcgatctcg
     1981 taaagtctgg ggaacttggt ggtcagtata tgcttgcggg cacagttcac ggatcaaca
//
LOCUS       DAOQUC010000775         2038 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_70304_length_2038_cov_3.053959, whole genome shotgun sequence.
ACCESSION   DAOQUC010000775 DAOQUC010000000
VERSION     DAOQUC010000775.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70304_length_2038_cov_3.053959"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1350)
                     /gene="amrB"
                     /locus_tag="PKH07_12520"
     CDS             complement(<1..1350)
                     /gene="amrB"
                     /locus_tag="PKH07_12520"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HNT35806.1"
                     /translation="MNTFRWFGLALLSLVRKVIAMDRQWMLMLILSVGSTLVLLFFSK
                     VFAACAWGDQTTTKERIEVRPCSGKGFWFPESETELRSEVQEHIRMAQQKKMPGKIIG
                     LVAPHAGYSCSGSVAGKAYRQIHGNPYDVVVIIGLSHRYPISKASVQPVDFYETPLGR
                     IPIDKHSAMALLEFSNEFEYVKGAHAEEHSVENQIPFLQEAVPNLKIVPIVINKDSLA
                     FTERVADDLALVFKGKNVLFVCSTDMSHFPNYDDANRVDRAALEALKCFDLPLVDRKI
                     SELERQQVPMLDCVFCGKAALYTVMGAAKRLGADSVEIIEYQNSGDILGSKSRVVGYG
                     AVALCQKSKGDSNEIEQAQETSQPESMDRTIVTESIGLSPEEALLNDQQKSQLLNIAK
                     KTVTAVANGEEMGEVTSDDPALMQPRGAFVTLKKSGRLRGCIGSLEARRPLAVDVRAN
                     "
     gene            1825..>2038
                     /locus_tag="PKH07_12525"
     CDS             1825..>2038
                     /locus_tag="PKH07_12525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35807.1"
                     /translation="MKKTLLTVLGIVLVGCVTLGLAAADSSGKADTRISIGIGTGSGY
                     FGFSYFDDSDWWWDDYRRDYYVVYYPC"
BASE COUNT          470 a          546 c          517 g          505 t
ORIGIN      
        1 gttcgcgcgc acgtccaccg cgagcggccg tcgtgcctcc aggctgccga tgcacccgcg
       61 caggcgtcca ctcttcttga gcgtgacaaa cgcccctctg ggttgcatca gtgcgggatc
      121 gtccgatgtc acttcgccca tctcctctcc atttgccaca gcggtcacag tcttctttgc
      181 gatgttgagc agctgtgatt tctgctgatc gttcagcaaa gcctcttcgg gagaaagtcc
      241 gatggactca gtgacgatag tcctgtccat tgactctggt tgtgaggtct cttgtgcctg
      301 ctcgatctca ttgctgtccc ctttggactt ttgacacaga gccactgcac catagccgac
      361 aactctggac ttgctgccaa gaatatcacc agagttctgg tattcgataa tctcgacgct
      421 gtcggctcca agccgtttgg ccgcgcccat gacggtatag agagccgcct tgccacagaa
      481 aacgcagtcc agcatgggaa cctgttggcg ttccaactcg gagatcttcc ggtcaacgag
      541 tggcagatca aagcacttga gcgcttcgag tgccgccctg tccactctgt tggcgtcatc
      601 gtagttcggg aaatggctca tgtctgttga acagacaaac aggacgttct tccctttgaa
      661 cacaagggca agatcatcag ccactctttc ggtgaaggcg agtgaatcct tgttgatgac
      721 aatcggaacg atcttcaggt tcgggactgc ttcctgcagg aagggaatct gattctcaac
      781 cgaatgctct tcagcatgag ctcccttgac gtattcaaac tcgttggaaa actccagcag
      841 cgccatggct gagtgtttgt ctatcggtat ccgcccaagt ggtgtctcgt agaagtccac
      901 cggctgaaca gaggcttttg aaatcgggta tctgtggctg agtcctatga tgacgacaac
      961 atcatagggg ttaccatgaa tctgccgata ggcttttccc gcgacacttc ccgaacagga
     1021 atatcccgca tgtggagcaa ccagcccaat gatcttgccg ggcatctttt tctgctgagc
     1081 catcctgata tgttcctgaa cttcgcttcg caactcagtt tcagactcag gaaaccaaaa
     1141 ccccttgcca ctgcatggtc gaacttcgat gcgctccttg gtggttgtct gatcacccca
     1201 cgcacaggca gcaaaaacct ttgagaagaa caacaggacg agggtggaac cgactgacaa
     1261 gataagcatc aacatccatt gtctgtccat cgcaatcacc ttcctgacaa ggctaagcag
     1321 agccaaaccg aaccatctga acgtgttcat cttctatcct gataccaact accgcgccga
     1381 cagaatgcag cccgcgacgc cttaccttgg cactttaggc ttgcttgctc tcctctgtca
     1441 aatcaaatct tccgttcgag ggatttgcga taacctttgt gcagcgccaa ccgtcattcc
     1501 aaacgggggc agagcggggt gaacaatccc tctgattgca atagccttat gttgagaaga
     1561 gagatccctc gcggagttca tcctgagcca agtcgaaggg ctcggagcaa ccgaatgagt
     1621 tgattctggt aactctcgca atgccttcgc gaacagtttg cgaaagagga acttgcattc
     1681 cgaacgagtt cgtgtgtaac cttttcgact gagcaggaca ctgcaatgct ctctgagttc
     1741 tgtctacttt ttgaataaga ccgacagtca actcgtcgga taggcagaac tgatagaaag
     1801 gatgagaaaa ggaggtacag aaccatgaaa aagactcttt tgacggttct gggaattgtt
     1861 ctggtaggtt gtgtcactct gggtctggca gctgcggact caagtggtaa ggccgataca
     1921 aggattagca tcggtatcgg aacggggagc ggctacttcg gtttcagtta tttcgatgat
     1981 tccgactggt ggtgggacga ctaccgaagg gactactatg tggtctacta tccctgtt
//
LOCUS       DAOQUC010000776         2037 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_70363_length_2037_cov_2.741675, whole genome shotgun sequence.
ACCESSION   DAOQUC010000776 DAOQUC010000000
VERSION     DAOQUC010000776.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2037
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70363_length_2037_cov_2.741675"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1129
                     /locus_tag="PKH07_12530"
     CDS             <1..1129
                     /locus_tag="PKH07_12530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35808.1"
                     /translation="VSQCDGRAFRLISVEAEAEGVNDESYKLAVSRAQQTAVEQALGL
                     LIPRSRIEANRQALLDAILYHAEQYTATSEITFTFRRDRDLSNIDDIGSSIVGARYHV
                     DMGALENKLDEVGLLGESEPVATVIFALDCEVDGVVFDATAAREIFVRELAANAFKLL
                     DSEEIRSTLHGTRSEGTEQEDWAFEVGKEFAVDYVLLVKGSGLAQEKSVLLTKTLYPC
                     DTTLTARVFDLRTRKISDSEEFSGKSEAETLSVAAADGFSNAADFIARTLFPRVRTAW
                     RERYWKGNTIQIDLSEVNYASCHKLLRELRSLSWVNSARRVKLVDDTAVLSVTVRDDS
                     AGLANTMIHLPGISMELVEVSATKIVAKTKPPKRILPVRKD"
     gene            1141..1842
                     /locus_tag="PKH07_12535"
     CDS             1141..1842
                     /locus_tag="PKH07_12535"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNT35809.1"
                     /translation="MDCNQQVSAIVQQAKACCVVMRQLGSATKSGVLLEVARQIRSRG
                     DFIQQENRKDLEFGRSKGLSPALLERLELTPKRIEEMATGVEEIVALPDPVGEVTRMW
                     RRPNGMQVGRMRVPLGVIAIIYESRPNVTIDAASLCFKSGNVSVLRGGSEAAHSNEAL
                     ISVFRRACVAKGVPAEVVSSLGTTERDAVQALLKMDAYVDVVIPRGGKSLIRNVMENS
                     TIPVIKHYEVSVISL"
BASE COUNT          458 a          495 c          603 g          481 t
ORIGIN      
        1 tgtatcccaa tgcgacggca gggcattcag actgatttcg gtggaagcgg aagccgaggg
       61 ggtcaacgac gaatcgtaca aactggcggt ttcacgtgcg caacagaccg ccgttgaaca
      121 ggccttgggt ctgctcattc cacgtagtag aatagaagcg aatcgtcagg ctcttctcga
      181 cgcgattctc taccatgccg agcagtatac ggcgacctct gagattactt ttacgttccg
      241 acgtgatcgg gatctgtcca acattgacga catcggcagt tctattgttg gagccaggta
      301 tcacgtggac atgggcgccc ttgaaaacaa actggatgag gttggtcttc tgggtgagag
      361 cgagcctgtc gctacggtca tattcgcact ggattgcgaa gtggacgggg ttgtgtttga
      421 tgccacggct gcccgtgaga tctttgtgag ggagttggcg gcaaacgctt tcaaacttct
      481 tgactctgag gagattcgaa gcaccctcca tggaacacga tccgaaggca cagagcaaga
      541 ggattgggct ttcgaagttg gcaaggaatt tgctgttgac tatgtcttgt tggtgaaggg
      601 tagcggactt gctcaggaaa agtcggtgct tctgaccaag acactttacc cctgtgatac
      661 tacgctcaca gcccgtgtgt tcgatttgcg gactcggaag atctccgatt cagaggagtt
      721 ctccggaaaa tcagaagcag aaactctgag cgtcgcggct gccgacggtt tcagcaatgc
      781 ggcggatttc attgccagga cacttttccc acgtgttcgc accgcatggc gtgaacgata
      841 ctggaagggt aacaccatac agatcgatct gtctgaagta aactatgcga gttgccacaa
      901 gctgcttcgt gaactgcggt cgctcagttg ggtgaattct gccagacggg tgaaactggt
      961 ggatgacact gccgtgctaa gtgtcaccgt tcgcgacgat tccgctgggc tggccaacac
     1021 gatgattcat ttgccaggta tctccatgga gttggtcgaa gtaagcgcaa ccaagatcgt
     1081 tgccaagacc aaaccaccca agaggattct gcccgttcgg aaggactgac acgcatactc
     1141 atggactgca atcaacaggt cagcgcgatt gttcaacagg cgaaagcctg ctgtgttgtg
     1201 atgcgtcagc ttggctctgc cacaaagagc ggcgtgcttc ttgaggtggc gaggcagatt
     1261 cgttctcgtg gcgacttcat ccaacaggag aatcgaaagg atctcgagtt tggcagaagc
     1321 aaggggcttt caccggcgct cttggagcgc ctggaactca ccccgaagcg aattgaggaa
     1381 atggccacgg gcgttgagga aattgttgcc ctgcccgatc ctgtcggtga agtgacacga
     1441 atgtggcgca gacccaacgg aatgcaggtc ggaaggatgc gcgttccact gggtgttatt
     1501 gcaatcatct acgagtcgcg tcccaatgtt accattgacg ctgcgtcttt gtgtttcaag
     1561 tctggcaacg tctctgtttt gcgtgggggt tccgaagcgg ctcattccaa cgaagcattg
     1621 atctcagtct tcaggagagc gtgcgttgcc aagggtgttc cggccgaagt cgtttcctcg
     1681 ctgggaacga ctgaacgtga tgcagttcaa gcgcttctga agatggatgc ctatgtggac
     1741 gttgtcatcc cacgcggtgg caagagtctc atccgaaatg tgatggagaa ctccactatt
     1801 ccggtcatca agcactatga ggtatctgtc atctctttgt agacagagcg gccgatctgg
     1861 aaatggcgct gcggctgact gtgaacgcca aggttcaacg gccaggaacg tgtaacgctt
     1921 tggagacgct tctggttcat cgagaagttg ctgcggagtt tctgccaaag gtctgtgacg
     1981 ccttgactca agcgggtgtc cagatgcttg ggtgcgaaga gacgcggagc atctgcc
//
LOCUS       DAOQUC010000777         2037 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_70366_length_2037_cov_2.603431, whole genome shotgun sequence.
ACCESSION   DAOQUC010000777 DAOQUC010000000
VERSION     DAOQUC010000777.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2037
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70366_length_2037_cov_2.603431"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2037
                     /locus_tag="PKH07_12540"
     CDS             <1..>2037
                     /locus_tag="PKH07_12540"
                     /inference="COORDINATES: protein motif:HMM:NF024930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35810.1"
                     /translation="TPTASPTETPLATETPTPVETPTETQTFTPTLTDEPTHTPTQTS
                     TNVPTSTDSPTVTPTFSATSTPTALQTGSIVAWGSNNFGECDVPEPNENFIKIAAAGE
                     AHSLGLKSDGSVVAWGRNLYGACDVPSPNSGFKEISAGMDFSLGIKTDGSIVAWGWNG
                     LGQCNVPAPNTAFKAVSGGELHALGLRASGTIATWGDNRYGQLNVPSPNSGFTKISAG
                     GVHSLGLKENGSVVAWGASSYNYGQCNVPAPNSDFVAISAGGYHSLGLKRDGSIVAWG
                     LNTSGQCNVPSPNSDFTAIVAGFTHSLGLKSDGTIVAWGANHAGQCNVPTPNSHFIAI
                     AASFNHSLGLRSVVEQPTETPTPTPTEINEAPTLESVYLTPDPGRVAQPLTAVPAGFH
                     DPEGAAAAYRYEWFVNGTEIAGQDSDVLQPHFFADGDLVMVLVYPFDGKIYGEPVSDS
                     VRIVSGAFTLSAIPEELTLANGQSGEFVIRVFPTVPESMSVALELIAGNTDGIRAEFE
                     PSRVDVQAGQPFGFALLRVDVYNFAQSGARPLTIRGVSAFWQDEVTVVLNIIPKKPTQ
                     SILTLNVTPTQVDVAGSIRVYGDLLPAASGAVINIQIRGDFSADYQTQTDTQGHYELR
                     LQMLNAGAVQVQAFAPQQSAQSRQVSVTVGRFQSVSIILKTNATGEELVGDTVNI"
BASE COUNT          465 a          580 c          547 g          445 t
ORIGIN      
        1 actccgacag cctcgcccac ggagacacca ctggcaacgg agactccaac accagttgaa
       61 acaccaacag agactcaaac attcacgccg acgttaacag acgaacccac tcatacgcca
      121 acacagacca gtacaaacgt gccaacatcc acagactctc cgaccgtcac tccgacgttc
      181 tccgcgactt cgacaccgac tgccttgcag accggttcca tcgttgcctg gggaagtaac
      241 aactttgggg agtgtgatgt ccccgaaccc aacgagaact tcatcaagat tgccgcagcc
      301 ggagaagccc actctctcgg cctgaagtcg gatggttctg tcgttgcctg gggaaggaac
      361 ctttacggag cctgcgatgt gccctctccg aacagtggct tcaaagagat ctcagcgggg
      421 atggacttca gtttggggat aaagacggat ggttcgatcg ttgcttgggg atggaacggt
      481 ctcggacaat gcaatgtgcc tgcacccaac actgccttca aagccgtttc tggcggcgag
      541 ttgcatgctc tcggactgag agccagtggt acaatcgcca catgggggga taaccgatac
      601 ggccaactga atgttccttc gccaaacagc ggattcacga agatatctgc cggtggggta
      661 cacagccttg ggctaaagga gaacggctcc gttgttgctt ggggagcctc gagttacaac
      721 tatggtcagt gcaatgtgcc tgctcccaat tcggatttcg ttgccatctc tgccggtgga
      781 tatcacagtc ttggactgaa gagagacggc tctattgttg cctggggact gaacacttcc
      841 ggccagtgca acgttcccag tccaaactct gactttacgg cgattgtggc aggattcacc
      901 cacagtctcg gtctgaagtc cgatggaacc attgtcgcct ggggtgcaaa ccacgcagga
      961 caatgtaatg tgccgacacc caattctcac ttcatcgcca tagcggcatc cttcaaccac
     1021 agccttggac tgagatcggt tgtcgaacaa cccacggaga cacccactcc cacaccgacc
     1081 gaaatcaacg aggcaccgac actcgaatcc gtctatctca cgcccgaccc aggcagagtc
     1141 gctcagccac tgaccgctgt tcccgctgga ttccatgatc cggaaggggc tgccgctgcc
     1201 tatcggtacg agtggtttgt gaatggaacg gagatcgctg gtcaagactc ggatgtgctg
     1261 caaccgcact tttttgctga tggggacctg gtcatggtac tggtctatcc ctttgacgga
     1321 aaaatctatg gagaaccggt ctctgattcc gttcgaattg tgtcaggggc gttcactctg
     1381 tctgccattc ccgaagaact cacactcgca aatggacaat caggggaatt cgttattcgt
     1441 gtctttccga ctgttcccga gagcatgtct gttgcgttgg aactgatcgc tggtaacacc
     1501 gacggcattc gcgcggagtt tgaaccgtct cgagtggacg ttcaagcggg gcagccgttc
     1561 ggttttgctc tactgcgtgt ggacgtttac aacttcgcgc agtcaggagc acgacctctg
     1621 acgatccgcg gggtcagtgc gttctggcag gatgaagtga ccgttgtcct gaacatcatt
     1681 cccaagaaac cgactcagag catcctgact ctgaacgtga ctccgacaca ggttgacgtg
     1741 gcaggtagca tacgcgtcta tggcgatttg cttcctgctg cgagtggcgc cgtgatcaac
     1801 attcagattc gcggtgattt ctcggcggac tatcagacgc agacggacac tcaagggcac
     1861 tatgaactgc ggcttcagat gctgaacgcg ggtgctgtcc aggtacaggc ttttgctcca
     1921 caacagagcg cccagagcag acaggtatct gtcaccgttg gtcgtttcca gagcgtcagc
     1981 atcatcctca agaccaacgc cacaggtgaa gaactggttg gggacaccgt caacatc
//
LOCUS       DAOQUC010000778         2035 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_70470_length_2035_cov_2.740909, whole genome shotgun sequence.
ACCESSION   DAOQUC010000778 DAOQUC010000000
VERSION     DAOQUC010000778.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2035
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70470_length_2035_cov_2.740909"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2035
                     /locus_tag="PKH07_12545"
     CDS             <1..>2035
                     /locus_tag="PKH07_12545"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNT35811.1"
                     /translation="KKEIPEIEKTLPAGVRINPFYDRTELIQACIRTVSDALLQGGIL
                     VILVLFLFLGDFRAALTVALSLPLMVSVAFVLMGWKGITANLMSLGGLAIAVGLIADG
                     AIIVAENITRHLQDSSGRHLPKNIIAAQALKEIIRPLFFAICVIVVAVLPLFSLQAHE
                     GKMFKPLAMAVLFAMVGSLIVSMTIVPILTSLLTKRGQERERRDLILQAILAVHRPVL
                     RLALWGGWSTLAVAAAILVASFALIPGIGTEFLPALDEGAIAINVVRLPSAGLEGSKI
                     QALLMERRLKERFPEIESVVSKTGRAEIAEDPMGPEQNDLILSLKPKDQWTSARTKDE
                     LVRLISLELAKIPGIRPAFSQPIALRVNELISGIKSDVAIKILGDDLEELQSIGAEIA
                     PILSTIEGAEDVKLEQVSGFSQTEIQYDRRSLARHGISVSEVNEIIETAVGGRVATEL
                     YEGQRKIAVLVRFPEQFRRDRETLERLLVVSPMGYRVPLADLAEIVEVDAPAQISREN
                     SQRRLLVECNVRGRDIGGFIREAQECTQEISDSLPEGYRLVWGGQFENQQRAAARLRI
                     VVPIALLLIFVLLYTSLNSLKSAFLVYMNLPFAVVGGLLALHVLGINFSVAASIGFIA
                     LLGVAVEDGLLLVSFFDQLLANGYPVREAVFEGCRLRLRSLLMTTMTTVLGLLP"
BASE COUNT          435 a          542 c          571 g          487 t
ORIGIN      
        1 aagaaggaga tcccggaaat cgagaagacc ctgcctgccg gagttcgtat caaccccttc
       61 tacgatcgca cagagttgat ccaagcctgc attcgaaccg tctcggacgc cttgctgcag
      121 ggagggattc tcgtcatact ggtattgttc ctgtttctcg gagacttccg tgccgcactg
      181 accgttgcgc tctcactccc cttgatggtt tcggttgcct tcgttctcat gggatggaaa
      241 ggtataacgg caaacctgat gagtcttgga ggactggcaa ttgcggtcgg actaatcgcc
      301 gacggtgcca tcatcgtagc cgagaatatc acacgccatc tccaggattc ctcgggcaga
      361 catctcccca agaacatcat tgcagcgcag gcattgaagg agattatacg tccccttttc
      421 ttcgcaatct gcgtgatcgt ggttgctgtc ctgcccctgt tctctcttca ggcacatgag
      481 ggcaagatgt tcaaacccct ggccatggcg gtgctgtttg ctatggtcgg gtcgcttatc
      541 gtctctatga caatcgttcc aatcctgact tcactgctga ccaaacgcgg ccaggagcgg
      601 gaaagaaggg acttgattct ccaggcaatt ctggctgtcc accgccctgt tctccgtttg
      661 gcattgtggg gaggatggag cacactagcg gtcgccgcgg cgatcctcgt cgcatccttt
      721 gccttgatac ctgggatcgg aacggagttc ttgcctgcct tggacgaagg tgccattgcc
      781 atcaatgtcg tgcgtctgcc atcagcgggc ttggaaggat ctaagataca ggctcttctg
      841 atggaacgcc gactgaaaga gagatttccg gaaatcgaaa gcgtggtcag caagacagga
      901 cgtgcggaga tcgccgagga tccgatggga ccggaacaga acgatctgat cctgagcctc
      961 aagcccaagg atcagtggac gtcggcacgc acaaaagacg aacttgtcag actcatcagt
     1021 ctggaactgg caaagatacc cgggatacgt cctgcattct ctcagccaat tgctcttcgc
     1081 gtcaatgaat tgatcagcgg gataaagtcc gatgtggcga tcaagatact tggagatgac
     1141 ctcgaagaac ttcagtccat tggagccgag attgctccca ttctatccac catcgaaggt
     1201 gccgaagacg taaaactgga gcaggtctcc ggattctctc agaccgaaat ccagtatgat
     1261 cggaggtcct tggcgagaca cggcatcagt gtctccgaag tcaacgagat cattgagaca
     1321 gcggttggag gtagagtggc aacggagttg tacgagggac agcggaaaat cgccgtcctg
     1381 gtacgattcc cagagcaatt ccgtcgggac agagaaacac ttgaaagact cctggtggtg
     1441 tcccccatgg gctacagagt tcctctagca gacttggctg agatcgtcga agtggatgct
     1501 cccgcccaga tcagccgaga gaattcccag cgacgcctcc tggtcgaatg caacgtgcga
     1561 ggccgtgata tcggaggatt catccgagag gctcaggagt gcactcagga gatcagcgat
     1621 tccctgccgg aaggatatcg gctggtgtgg ggtgggcagt ttgaaaacca acaacgcgcc
     1681 gcagcacgtc ttcgaattgt cgtccctatt gcgctgctgt tgatcttcgt tctgctctac
     1741 acatctctga actcgctcaa gagcgcattt ctggtttaca tgaatcttcc cttcgcggtg
     1801 gtgggaggtt tgcttgcctt gcatgtgctt gggatcaatt tcagtgtggc tgccagcatt
     1861 ggtttcatcg cgcttctcgg tgtggctgtc gaagatggtc tgctgttggt cagtttcttt
     1921 gatcaactgc tcgccaacgg ctatcctgtt cgcgaagccg tcttcgaggg atgccgcctt
     1981 cggcttcgct cgctgctcat gaccacgatg acgaccgtgc tgggtctgtt gccgt
//
LOCUS       DAOQUC010000779         2033 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_70559_length_2033_cov_3.222447, whole genome shotgun sequence.
ACCESSION   DAOQUC010000779 DAOQUC010000000
VERSION     DAOQUC010000779.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70559_length_2033_cov_3.222447"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2033
                     /locus_tag="PKH07_12550"
     CDS             <1..>2033
                     /locus_tag="PKH07_12550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35812.1"
                     /translation="SRLYVDSNAAGTNTGLNWSDAYTDLRDALDLANSCAAVKEIWVA
                     AGTYKPDRGTSDRSQSFAINGGVKVYGGFEGVLASSYPGGETQLRQRDWRANRTVLSG
                     RIGTSLNSYHVVTVFNAKGYCWLDGFTIEGGNANGVSGGIDYFGGGLCAESSQIYINN
                     CAFVENTASQLGGGCYLYNCGASVVNCFFGRNNATYGAGLYAQVSNLQTYNSVFSGNM
                     AQNGAGLYLFFSEANIVNGTLYGNTASNNGGGCLIQTDRPIAFKNTILWHNQDKNGSG
                     QSAQMHFASGTATVNHSCVQGWLGTLTGVKNTSEDPMFMNPPGADGILGTLDDDLRLS
                     AMSPCIDSGNNSYVAADFADFNHNGNTTEKLPFDFAGKERLLDDPLMENTGTGTPPVD
                     MGAFEFYMDCNNNGVPDYIEIANDPSLDGDGNSILDECNTFVGDELVPPLGGADLAFE
                     PYVSNGNIAFVHPFDRKVYAKAPGTVKVSWARAGIPRSITGNYTVMTTPGTVTAYITS
                     APYNSPKVKMEALKKGTPPVDVVFHYNSTIKDDATPSDPLTTADAYLDILDQMSVEKV
                     GQILLEYRNRVTGEKLDFEIMDVKNPVPVISAGVEVGTELLPSSPDKTDENCHPYVSL
                     GLIGTPYVYQQGAVQPGPTQYKVYAIRPSSNSPNRIEIFWYKTGKWDVQWPSEV"
BASE COUNT          500 a          526 c          548 g          459 t
ORIGIN      
        1 tccaggctct acgtggacag caatgccgca ggaaccaaca cgggtctcaa ctggtcggac
       61 gcctacacag acttgcggga tgctcttgat ctggccaatt cgtgcgctgc ggtcaaagag
      121 atttgggttg ctgcgggcac atataagccc gatcgaggaa caagtgatcg ttcccagagt
      181 tttgccatca acggaggagt gaaggtctat ggtggctttg aaggggtcct tgcgtcgtcg
      241 tatcctggag gcgaaaccca actgagacag agagactggc gagccaacag aacggttttg
      301 agtgggcgca tcggaacctc cctcaactcc tatcacgttg tgacagtgtt taatgcaaag
      361 ggatactgct ggctggatgg tttcacaatt gaaggtggca atgccaacgg agtttcaggt
      421 ggcattgact attttggggg cggtctgtgt gctgaatcct cccagatata catcaacaac
      481 tgtgccttcg tggaaaacac ggcgtctcag ttgggaggag gctgttatct gtacaactgc
      541 ggggcatcag tggtcaactg cttctttgga agaaataacg caacttacgg tgctggactc
      601 tatgcgcaag tctccaacct gcagacctac aactccgtat tcagcggtaa catggcgcag
      661 aatggtgctg gcctgtatct gttcttcagt gaggctaaca ttgtcaacgg aaccctctat
      721 ggtaacactg cctccaacaa tgggggtggc tgtctcattc agacagatcg ccccatagcc
      781 tttaagaata cgattctttg gcacaatcag gacaagaatg gctcaggcca gtctgcacag
      841 atgcactttg cttcgggcac tgcgacagtc aatcactcat gcgtccaggg atggcttggc
      901 actctgaccg gagtcaagaa tacatcggaa gacccaatgt ttatgaatcc cccgggagcc
      961 gatggcatcc ttggaacctt ggatgacgat ctgcgactga gtgcgatgtc tccctgtata
     1021 gacagcggaa acaactccta tgtcgctgcc gacttcgctg atttcaacca caacggcaac
     1081 acgacagaga aactcccatt cgacttcgcc gggaaagaac gccttctgga tgatcctttg
     1141 atggagaaca caggcactgg gactcctccc gtggatatgg gtgccttcga gttctacatg
     1201 gactgcaaca acaacggagt tccggactac atcgagatcg ccaatgatcc gagccttgat
     1261 ggtgacggca acagcatcct ggatgaatgc aacaccttcg ttggagatga actggtgcca
     1321 ccgcttggag gcgctgacct ggctttcgaa ccgtacgtca gcaatggaaa tatcgcgttc
     1381 gtccatccgt tcgatcggaa ggtctatgcc aaggctccag gaaccgtgaa ggtctcttgg
     1441 gcaagggcag gtattcctcg gagtatcacc ggaaactaca ctgtcatgac gacccccgga
     1501 acggtcactg cttatattac cagtgctccg tacaactcgc ctaaggtgaa aatggaggcg
     1561 ctcaagaagg gaactccgcc cgtggatgtt gtcttccatt acaactcgac aatcaaggat
     1621 gacgcaactc caagcgatcc gctcacgacc gccgatgcct atctggacat cctcgatcag
     1681 atgtcggtcg agaaggtcgg tcagattctt ctggagtacc gaaaccgagt gacgggagag
     1741 aaactcgact ttgagatcat ggacgtcaag aatcctgtcc cagtcatttc ggcaggagtc
     1801 gaagtcggaa cggaactact tccatcgtca cccgacaaga cggatgaaaa ctgtcatcct
     1861 tatgtatcgc tgggcttgat cggaacgcct tatgtgtatc agcaaggtgc agttcagccc
     1921 ggtccgactc agtacaaggt ctacgcgatt cgtccctcga gcaatagccc caatcgcatt
     1981 gagatcttct ggtacaagac aggcaagtgg gatgtgcagt ggccttcgga agt
//
LOCUS       DAOQUC010000780         2033 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_70567_length_2033_cov_2.871082, whole genome shotgun sequence.
ACCESSION   DAOQUC010000780 DAOQUC010000000
VERSION     DAOQUC010000780.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70567_length_2033_cov_2.871082"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1018)
                     /gene="gltX"
                     /locus_tag="PKH07_12555"
     CDS             complement(<1..1018)
                     /gene="gltX"
                     /locus_tag="PKH07_12555"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HNT35813.1"
                     /translation="MSQTVRTRFAPSPTGQMHIGNVRTALFAWLFARANKGKFILRIE
                     DTDQSRSTDEYIQAILNDLKWIGLDYDEFHRQTERADIHRQLAQQLLQEGKAYRCYCT
                     PEQLEQRRKEMSERSENIGYDRRCRDLAEQPQDVPCALRLKIPLEGVTEFDDLVFGKV
                     AVQNKEIEDLVILRSDGSPTFNFACALDDHAMGITHVIRGDDHVANTPKQINILRAFG
                     YPIPQYAHLSSILGPDKARLSKRHGATALDWYRQNGYLPDAMVNYLVRLGWAFGDEEF
                     FRRQDLIEKFSLDHINRSAAVFNPEKLLWLNGEHIRSTTPADLWHLWKPFVLDAGLAE
                     VVERT"
     gene            1175..1807
                     /locus_tag="PKH07_12560"
     CDS             1175..1807
                     /locus_tag="PKH07_12560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35814.1"
                     /translation="MKRIVIIGLLVLSVCGPVAECNAMSPVTRYLKNRWNDFLDLFIV
                     EVGMPQRHLAWGAHLRLTDMLQVGQIHFQGVKAGLDGRALIVVNEEKDQRSIPLYESY
                     TEIDQTLRWSRPDQTQWLGRPIIRNGRYEWNDGRDMPWSVGLEIMFPPCFGGLELGLA
                     FCQLGDFAFGLLNLDPWNDDIKPMQPTPEELILTAPVPQATVPGSRSSLE"
BASE COUNT          433 a          492 c          573 g          535 t
ORIGIN      
        1 ctgtgcgctc cacgacctct gccagccctg catcaaggac aaacggtttc caaagatgcc
       61 acaggtctgc gggagtagta gaccggatgt gctctccatt gagccaaaga agtttctccg
      121 gattgaacac tgctgcagag cggttgatat gatcgaggga gaacttttcg atgagatcct
      181 gtcgtctgaa gaattcctca tctccgaacg cccaacccag tcgaaccagg tagttgacca
      241 tggcgtcggg caaataccca ttctggcggt accagtccag agcggtggct ccatgtctct
      301 tgctcaatcg tgctttgtcc ggtcccagga tgcttgaaag atgtgcatac tggggaatcg
      361 gatatccgaa cgccctgagg atgttgatct gcttgggcgt gttggcgaca tgatcgtccc
      421 ctcggatgac gtgggtgatt cccatggcat gatcgtccag agcacatgcg aagttgaaag
      481 tcggcgaacc atcgcttctg aggatgacca gatcctcgat ttccttgttc tggacggcga
      541 ccttgccaaa aaccaagtcg tcgaattcgg tgacaccttc caaaggaatc ttgagtcgga
      601 gagcacaggg aacgtcctga ggttgctctg cgagatctct gcaccttcgg tcatagccga
      661 tgttctcact tcgctccgac atctccttgc ggcgttgctc aagctgttcc ggcgtgcaat
      721 aacaccggta tgccttgcct tcttgaagaa gttgctgtgc cagttgtcgg tggatatcgg
      781 ctctctcggt ctggcgatgg aattcatcat agtccagacc aatccatttt aagtcattga
      841 gaatggcctg gatgtactca tccgtggaac gagactgatc cgtgtcctca atgcgcagga
      901 tgaatttccc tttgttggca cgcgcaaaca accaggcgaa aagagcagtt cggacgtttc
      961 cgatgtgcat ttggccggtt ggggaagggg caaatcgagt tctgacagtc tggctcaagg
     1021 aagacctcca tctacggttg tgattcgtga atccttcaaa atgctgaaga aagcaccttt
     1081 tttcttgaaa ccgtattaag tttcatatag attattcaca gctttgtggg aaaagcaaca
     1141 tggaaaagat cgctccaagt tcaggaggct tttgatgaag cgaatagtga ttatcggttt
     1201 gttggttttg tcggtctgtg gccccgtcgc ggaatgcaac gcgatgtcac ctgtcacgag
     1261 atacctcaag aatcgctgga atgatttcct cgacctgttc atcgtcgagg tgggcatgcc
     1321 ccagcgacac ttggcttggg gcgctcatct gcgtctgacg gatatgctcc aggttggaca
     1381 gattcacttt cagggcgtca aggcagggct ggatggtcgc gctttgatcg ttgtcaatga
     1441 ggagaaagat cagcgcagca tccctcttta cgagagctat accgagatcg atcagacgtt
     1501 gcgatggtct cggcctgatc agacgcagtg gctcggtcgt cccattatcc gcaatggtag
     1561 atacgagtgg aatgatggca gggatatgcc ttggtctgtg ggtcttgaga ttatgttccc
     1621 tccctgtttc ggagggctcg aactgggcct ggcattctgt cagttgggtg attttgcatt
     1681 tgggcttctg aacttggatc cttggaatga tgatatcaag cccatgcagc ctacacccga
     1741 agagttgatt ttgacggcac ctgtgcccca ggcgacggtg ccgggatctc gctcaagtct
     1801 tgaatagatc atttcgggag actggtttct cccgatatct gtaattgttt cttagtcggg
     1861 caggcttgtg cgattcacct tgcccgtttc aggtgagcag aaagtcgtca aagcgaccaa
     1921 ggaggtagaa aatggtgcgg cgattgttgt gggttctgac cttgctggct cttctgccgg
     1981 cgggcacgca ggctgctggt ttctggcctg cagttgggga ctattttggc gat
//
LOCUS       DAOQUC010000781         2031 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_70658_length_2031_cov_3.506579, whole genome shotgun sequence.
ACCESSION   DAOQUC010000781 DAOQUC010000000
VERSION     DAOQUC010000781.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70658_length_2031_cov_3.506579"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..899)
                     /locus_tag="PKH07_12565"
     CDS             complement(<1..899)
                     /locus_tag="PKH07_12565"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNT35815.1"
                     /translation="MTQDTSSNRKPKILVVDDEPAFKELICMWLRKRYDVIGADNAKQ
                     AMQELQANAFDLVLSDITMPGVNGYELVTEMRGLFPKMKVALITAWSIEECLKVALQQ
                     GIGNIIAKGLPLDFTELDLTVDRLLSEDIFGLERYLEYQTPVHKALVHSADEIIKVRE
                     EAFENLRIGDLPEQRQLALRLVVDEAIANAACHPRGDDLPRDGFVLPEEEAVEVHYAR
                     DLETIAFNVVDRFGNLNTQTILAYLERCINPTEDALTAERGRGLFLIRSLVDKMIINI
                     KKGVRTEVIMLVHPRQRPRDQRPL"
     gene            complement(905..>2031)
                     /locus_tag="PKH07_12570"
     CDS             complement(905..>2031)
                     /locus_tag="PKH07_12570"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNT35816.1"
                     /translation="LYSHTHGVIDNKGHQIAADQPIFSDLLREAGYEVAFCGKSHLKG
                     ALRDRKWDYYFGFQGQGAYRNPKIAEGADGKDEVHEGYMDDILTDKAIEWLTGRTGEK
                     PFCLFLWFKAPHRSWVRAERHKSLFENVVIPKPDTFDDDKKGFPGKPSAFANADNKIG
                     SFEDVQTLDGFVKDYYATLVAVDENLGRVFEVLQSKGIMDDTAILYTSDNGFFAGEWH
                     AFDKRFMHEPSIRVPLLIRYPRQVKARQTRRQMILNTDIAPTVLDLAGIAVPKWMHGM
                     SLVPVLQSKSADWRKDWLYEYYEFPGPHQVRKHRGIRTERYKLIHYYEAPEEFELYDL
                     KKDPREISNLYGKPECHKLTQQLLKRLSELRQETGDTLQE"
BASE COUNT          443 a          559 c          512 g          517 t
ORIGIN      
        1 aggggtcgct ggtctctggg tcgttgtctc ggatggacga gcatgatgac ctctgtgcgt
       61 acgcccttct tgatgttgat gatcatcttg tccacaagac ttcgaatcag aaacagcccc
      121 cgcccgcgct ctgccgtcag agcgtcctca gttgggttaa tacagcgctc gagatacgcc
      181 aaaatcgtct gggtattcaa attgccaaac cgatccacca cattgaaggc gatagtctca
      241 agatcacgcg catagtgaac ctcgactgct tcctcttccg ggaggacaaa accatcgcgt
      301 ggcagatcgt ctcccctcgg gtgacaggca gcattcgcga tcgcctcgtc aacgacgagc
      361 cgaagtgcca gctgacgttg ttctggcaga tcacctatgc gaaggttctc gaatgcttct
      421 tcacgcactt tgatgatctc gtcagcgctg tggacaagag ctttgtgcac aggcgtttgg
      481 tattccaagt atctttcgag cccgaaaatg tcctctgaca acaatctgtc caccgtcaga
      541 tccagttcgg taaaatccag tggaagcccc ttggcgatga tgttcccaat gccctgctgg
      601 agagcgacct tgaggcactc ttcgattgac caggcggtga tcaaggcaac cttcatcttc
      661 ggaaagagac ccctcatctc tgtcaccagt tcgtagccat tgacgccggg catggtgatg
      721 tcgctcagaa caagatcgaa tgcattggcc tgcaactcct gcatggcttg ttttgcattg
      781 tccgcgccga taacgtcgta acgctttcga agccacatgc agataagttc cttgaaggca
      841 ggctcatcat ccacgacgag aattttgggt tttctgttgc ttgaagtgtc ttgagtcatc
      901 tgtctcactc ttgcagcgtg tcgccggttt cttgtctcaa ctcactgagt ctcttgagca
      961 gctgttgggt caacttatgg cattcgggtt ttccatagag attgcttatc tccctgggat
     1021 ctttcttgag atcatacagt tcaaactcct cgggagcctc gtaatagtga atcagtttgt
     1081 aacgttccgt tcgaatgcct cgatgctttc gaacctgatg cggtccggga aactcgtagt
     1141 actcgtagag ccagtctttt cgccagtcgg cactcttgga ctgaagcacc ggaacaaggc
     1201 tcatgccgtg catccatttt gggacggcaa tacctgccag atcaagcacc gtcggggcaa
     1261 tatccgtgtt cagaatcatc tgtctgcgcg tctggcgcgc tttcacttgg cgcggatagc
     1321 ggatcaacag gggtactcgg atagagggct catgcatgaa acgcttgtca aaggcgtgcc
     1381 actcacccgc aaagaaccca ttgtccgaag tgtagagaat cgcggtatcg tccatgatgc
     1441 ctttcgactg cagtacttca aagaccctgc cgagattctc atccaccgcc acaagcgttg
     1501 cgtagtagtc tttgacgaaa ccatcaaggg tctgcacgtc ctcgaaggat ccaatcttgt
     1561 tgtcggcatt cgcgaatgcg gacggcttgc caggaaagcc cttcttgtcg tcatcaaaag
     1621 tatccggttt gggaatgacc acattctcga agagactctt gtgtcgttct gctcgcaccc
     1681 aggaacggtg cggtgccttg aaccaaagaa agagacaaaa gggtttctcc ccagtacggc
     1741 ctgtcagcca ttcgatcgct ttgtctgtga ggatgtcatc catataccct tcgtgaactt
     1801 cgtccttgcc gtccgcacct tcggcgatct tgggattgcg atatgctcct tgtccctgaa
     1861 agccaaagta atagtcccac tttcgatccc gcaaagcccc tttgaggtgg gatttcccac
     1921 agaaggccac ctcataaccc gcttcacgga ggagatcgga gaagatcggt tggtcggccg
     1981 caatctgatg ccccttgttg tcgatgactc catgcgtatg cgaatagagc c
//
LOCUS       DAOQUC010000782         2031 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_70688_length_2031_cov_2.637652, whole genome shotgun sequence.
ACCESSION   DAOQUC010000782 DAOQUC010000000
VERSION     DAOQUC010000782.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70688_length_2031_cov_2.637652"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2031
                     /locus_tag="PKH07_12575"
     CDS             <1..>2031
                     /locus_tag="PKH07_12575"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HNT35817.1"
                     /translation="EVHCVSEWGFEFRPDYLYAAQYVADWFRKMDLPGNPHRVLMLSA
                     TVTKRNQHDLERELRLPQPPDYDDLPRDMPHPIQPFIILESCELPETEELSADDKFSK
                     ILEILGKLDLAQSGALVFVRKREDCHRISEALNAAAAASGSAVAHLHSLPFHAGLPET
                     VKSEACDLLKARTVNVLVCTKAFGMGMDVPHLHACIHYRPPTFIEDYLQEVGRIGRSK
                     EERERTGHEQVTATLLFNHDNLEHNLGMLHDKAVKPPDLQDFFGYCLDRAVPFKQVGK
                     AVCIVPAALRINEAKSFDENQVTNCLFWLERMSVLRVEGRHPPFLDLTLRLSSLRKIS
                     ATGTDTAVVARALLSLVEETSRVVVDPEVADSSPASKQAAESAFSRFIRGLVRGVLAL
                     VSSPAQTSSVEDASARATARQIPSPANRDNIDVSVSMSEIMASSGGIGADDLFSRLIA
                     LSQANALSLRKTFVVLRSAVPSDKETYWPLLRYAVERLLRPTEGAVELLSRKAFEVEL
                     KAWYQAYLRQRDPPKDIEASAESGVPVARSAETPQKRRIQREVYRAIGGSLKLVRYAG
                     LRMKESLASDGTMQYARAIPDSSIAEVKRGISERLDAMQRLVAWLAKQEPQAGGGQAA
                     SFDVALVDLVNALGQETRVSRLRELMNLTEASGFYGLENKTDEWVSVVSLNT"
BASE COUNT          436 a          540 c          607 g          448 t
ORIGIN      
        1 atgaggtcca ttgcgtcagc gaatggggtt ttgaattccg tcccgattac ctgtacgcgg
       61 cccagtacgt tgctgactgg ttccgcaaga tggacctccc tggcaatcca catcgtgtcc
      121 tgatgttgtc ggcgacggtc acgaagcgga atcagcatga tctggagagg gaactcagac
      181 tgccacagcc tccggactat gacgatctgc cacgggacat gcctcaccca atccagccat
      241 tcatcattct ggaatcttgc gaacttccgg aaacagaaga gctttcagcc gacgataagt
      301 tcagcaagat attggagatt ctcggtaagc tcgacttggc tcaaagcggc gcgcttgtgt
      361 ttgttcggaa acgcgaggac tgtcatcgca tcagtgaggc gctgaatgcg gcggcggccg
      421 cttctgggtc cgccgtcgcc cacttgcatt cacttccctt tcatgcaggt ctgccggaga
      481 ccgtcaagtc ggaagcatgc gatttgctga aggctcggac cgtgaacgtc cttgtgtgca
      541 cgaaggcttt tggcatgggg atggacgtgc cacatctcca cgcctgcatt cattaccggc
      601 ctccgacctt cattgaagat tatttgcagg aagtcggtcg cattggcagg agcaaagaag
      661 agcgtgagcg cactggacac gagcaagtga ccgccacgct gctcttcaat cacgacaatc
      721 ttgagcacaa tcttgggatg ctccatgaca aggcggtgaa accgccggac cttcaggact
      781 tcttcggcta ttgtctagac cgcgccgttc cattcaagca ggtggggaag gctgtttgca
      841 tcgtacccgc cgcactccgc atcaacgagg caaagagttt cgatgagaat caagtcacga
      901 actgcttgtt ctggcttgag cggatgtcgg tgttgcgagt ggaaggacgt cacccgccgt
      961 ttcttgacct gactctgcga ctgagcagcc ttcggaagat ttcagctacc ggaacggaca
     1021 ccgctgttgt cgcacgcgca ctactcagcc tcgtcgagga gacttcgcgg gttgtggttg
     1081 atcccgaggt cgccgactca tcccctgcaa gcaaacaggc cgccgaatcc gcattcagcc
     1141 ggttcataag gggtctcgtg cgaggtgtcc ttgcgctggt gtcatcgcca gcacaaacct
     1201 cttcggtcga agacgcctcc gcaagggcca cggctcgaca gattccttcg ccagcaaatc
     1261 gtgacaatat tgacgtcagc gtgtcgatgt cggaaatcat ggcgtcaagc gggggcattg
     1321 gggcggatga tctcttctct cgtctgattg cgttgagtca ggcaaatgcg ctgtcgctgc
     1381 gcaagacttt cgtcgttctg cgatccgcag ttcctagcga taaggagacc tattggcctc
     1441 tactgcggta tgccgtagag cgcctgcttc gtccaacgga gggtgccgtt gagttgctat
     1501 cgcgtaaggc gttcgaggtg gaactgaagg cttggtatca ggcatatctg cggcaaagag
     1561 atccccccaa ggatattgag gcatctgccg aaagtggtgt gcctgtagcc aggtccgctg
     1621 agacaccaca aaagagacgc atacaaagag aagtataccg tgcgattgga ggatcgctga
     1681 agctggtccg ttacgcggga ctacgaatga aggagagtct cgcgagcgat gggacgatgc
     1741 agtatgcgcg agcgattcct gactcaagca ttgcggaggt aaagagaggc atctccgaga
     1801 ggctggacgc catgcagcgg cttgttgctt ggctggcgaa acaagagccg caggccggag
     1861 gcgggcaagc cgcttcattt gatgttgctc tcgtggatct cgtgaacgca ttggggcagg
     1921 aaacgcgagt ctctcgactg cgtgagttaa tgaacctgac tgaggcgtcc ggcttttacg
     1981 ggctggagaa caagacggac gagtgggtgt ctgtcgtttc tctgaacacg a
//
LOCUS       DAOQUC010000783         2030 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_70720_length_2030_cov_4.098228, whole genome shotgun sequence.
ACCESSION   DAOQUC010000783 DAOQUC010000000
VERSION     DAOQUC010000783.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70720_length_2030_cov_4.098228"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..635
                     /gene="pstB"
                     /locus_tag="PKH07_12580"
     CDS             <1..635
                     /gene="pstB"
                     /locus_tag="PKH07_12580"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010938383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HNT35818.1"
                     /translation="GKSTFLRAINRMNDLIPIARTQGQLIFDGQDIYGPQIDVVALRR
                     RIGMVFQKPNPFPKSVFDNVAFGPRIHGVKNHSQLTEIVEESLIRSALWDEVKDRLSE
                     NALGLSGGQQQRLCIARALAVNPEILLMDEPTSALDPRSTSRIEDLMEQLSQQYTIII
                     VTHNMQQAARVSDYTAFLYEGNLVEFGPTQSLFTKPQEKQTEDYVTGRFG"
     gene            672..1349
                     /gene="phoU"
                     /locus_tag="PKH07_12585"
     CDS             672..1349
                     /gene="phoU"
                     /locus_tag="PKH07_12585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014812780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HNT35819.1"
                     /translation="MSQHLQREIARLKKLILSLTAIVEDRVRHAVKSVKERNEEMAQR
                     VISGDIEIDQREVDIEEECLKLLALYQPVAADLRFIIAVLKINNDLERISDLAVNIAE
                     RAVFLARQPKIDVPFDFPLMAEKAQTMLRMSLDALVNWQSSMAMEVCAMDDEVDAINR
                     EMYNQIKDEIRLHPENLDSLIHLLSVSRHLERIADHATNVSEDVIYMIEGKIIRHRTE
                     DFAAGSN"
     gene            complement(1374..>2030)
                     /locus_tag="PKH07_12590"
     CDS             complement(1374..>2030)
                     /locus_tag="PKH07_12590"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HNT35820.1"
                     /translation="EPDKNAIRRNAQSVPKTLAKLRRGQKVVIVTWGDSVTAGGDAST
                     PDLAYPSLFITRLRERFPKSEIVHHNAGIGGSNTMGRLPNIEKEVLSFKPDLVTIEFV
                     NDMGFSEEVLRNNYTSAFEQIRAVGAEPLLITPHFVMPSWMDLPHPRGKETRKAITIL
                     RDLCVEQKVGVADTSQRWEHLDQEGIPYITLLVNGINHPDDRGHEMFVRDLLSFFPKR
                     "
BASE COUNT          515 a          538 c          519 g          458 t
ORIGIN      
        1 gtggcaagag cacctttctg agagccatca accgaatgaa cgacctgatc ccaattgcac
       61 ggactcaggg tcaactcatc ttcgacggac aggacatcta cggccctcag atagatgttg
      121 ttgccctgcg gagaagaatc gggatggtct tccaaaagcc caacccattt ccgaagtcgg
      181 tgttcgacaa cgtcgccttc gggccaagaa ttcacggagt caagaatcat tctcagttga
      241 ctgaaattgt ggaggaaagt ctcatacgct cagcactctg ggacgaagtc aaagatcggc
      301 tctcggaaaa tgcactaggc ttgtcaggtg gccaacaaca gcgcctctgc atcgccagag
      361 cactcgctgt caacccggag attctcctga tggacgagcc gacttccgca cttgacccac
      421 gctcaacttc cagaatcgag gacttgatgg aacaactcag tcagcagtac accatcatca
      481 tcgtgacaca caacatgcaa caggcagcac gagtttccga ctacaccgct tttctgtacg
      541 agggcaacct ggtcgagttt ggcccaacac agagtctctt caccaaaccc caggaaaagc
      601 aaactgaaga ttacgtgaca ggaagatttg gctagaattc cccacgagga agaaagttcg
      661 aggtttggaa catgtcccag cacctacagc gcgagatcgc ccgcctgaaa aagctcatcc
      721 tttccttgac cgccattgtc gaggacagag ttcggcacgc ggtaaaatcc gtcaaagaga
      781 gaaatgagga aatggcccag agagtcatct cgggagacat tgagattgat cagcgcgaag
      841 tggacatcga agaggaatgc ctgaaactgc tcgcgctcta ccaaccggtg gcagcagatc
      901 ttcgattcat catcgccgtt ctgaaaatca acaatgactt ggaaaggatt tcggacctgg
      961 cggtcaacat cgctgaacgt gccgtcttcc tggcgagaca gccgaagatt gatgttccct
     1021 ttgattttcc gctgatggcg gagaaagcgc agaccatgtt gcgaatgagc cttgacgctt
     1081 tggtcaattg gcagtcttct atggcaatgg aagtctgtgc catggacgat gaagtagacg
     1141 ccatcaaccg cgagatgtac aaccagatca aagatgaaat ccgacttcat ccggaaaacc
     1201 ttgattcctt gattcatctg ttgagcgtct cacgacatct tgagcgcatt gccgaccatg
     1261 ccaccaacgt ttctgaagat gtcatctata tgattgaggg aaaaatcatc cgacatcgaa
     1321 cagaggattt cgctgccggt tccaactgac gcggaaccct catccgccgc ctttcacctc
     1381 ttcggaaaga aggaaagcag atccctgaca aacatctcat gtccccggtc gtcgggatga
     1441 ttgatcccat tgacaagaag ggtgatgtaa ggaattccct cctggtcgag atgctcccag
     1501 cgttgggatg tatctgccac gccgaccttt tgctcaacac agaggtcgcg aaggatggtg
     1561 atggccttgc gcgtctcctt gccacgagga tggggaagat ccatccacga tggcatgacg
     1621 aagtggggtg tgatcaaaag cggttcagcc ccgacggcgc ggatttgctc gaatgcgctg
     1681 gtgtagttgt ttcgcaagac ctcttcggaa aagcccatgt cattgacaaa ttcgatcgtc
     1741 acgagatcgg gcttgaacga aaggacttcc ttttcgatgt taggcagtct gcccatggtg
     1801 ttgcttccgc cgatgcccgc gttgtggtgg acaatctcgc tctttggaaa tctctctcgc
     1861 agccgagtga tgaacaatga cggataggcc aaatcgggtg tggaagcatc tcccccagcg
     1921 gtgacactgt ctccccacgt tacaatgacc actttctggc ctctccgtag tttcgccaag
     1981 gtctttggaa cagattgagc attcctgcga attgcgtttt tgtctggttc
//
LOCUS       DAOQUC010000784         2028 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_70842_length_2028_cov_2.874303, whole genome shotgun sequence.
ACCESSION   DAOQUC010000784 DAOQUC010000000
VERSION     DAOQUC010000784.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70842_length_2028_cov_2.874303"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(221..685)
                     /locus_tag="PKH07_12595"
     CDS             complement(221..685)
                     /locus_tag="PKH07_12595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35821.1"
                     /translation="MDLVESLRLIAAKFDEEGIDYALCGGLAMAVYAMPRGTLDIDLL
                     IEERSLDRANQTLRVLGFEPTGVPMEFQSGKVFISRFRRIDCESGRVVVLDLVHVSAA
                     NSEAWKGRCDVEWEGSRLKVVSPQGLILLKSLRQSGQDQDDIEYLRGITDED"
     gene            complement(725..2002)
                     /gene="purD"
                     /locus_tag="PKH07_12600"
     CDS             complement(725..2002)
                     /gene="purD"
                     /locus_tag="PKH07_12600"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018702993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HNT35822.1"
                     /translation="MKVLVVGSGGREHCLAWKLASSSRVKEVLVAPGNAGTAQVGRNL
                     AVDSGNVTAICDSAVAEKVDLVVVGPEAPLSEGVVDALAKKGIPAFGPSGAAARLEGS
                     KSFAKEIMKRHGIPTAAFGIFDEYEPARCFLLEQYRGGREMVIKADGLAAGKGVFVPD
                     SQHEAERALHDVMVDRIVGEAGRQVVIEEKLKGEEVSVLALSDGETVVSLLSAQDHKR
                     VGDGDTGPNTGGMGAYSPVPRFDQAFLKQVHDTILLPTVRAMASEGHPYKGVLYAGLM
                     VDQGKPEVLEYNVRFGDPETQVILPLLESDLVDLAFACIEGNLASHSLRWRGGAALTI
                     VLASEGYPGSYPKGRPISGLSQVAQMSDVVVFHAGTALKDGNVITSGGRVLNVVGMGS
                     DMRSAQCKAYEAVSRIRFDGVYYRKDIGWRALA"
BASE COUNT          510 a          638 c          429 g          451 t
ORIGIN      
        1 ctccgaaaac aaaccccaat gccactggat tcagtgagcg caatcaggtt tctcatctgg
       61 gcgaacaaac cccagtgcca tcgcggtgcg ccgaaccttc tcattcgctg gatgtttctt
      121 gatgatctcc cgtcctgcac tggattttgc gagagacaaa cagatccgat tcagttccct
      181 cacttgttcc aaccgtcgag atatcgcttc tgaactcatg tcaatcttca tcagtgatac
      241 cccgaagata ctcaatatcg tcttgatcct gaccactctg tcgaagactc tttagaagga
      301 ttagcccttg gggcgacaca accttgagtc ggctgccttc ccactcgaca tcacaacgtc
      361 ccttccatgc ttctgaattg gcagcactca catgcaccaa atccagaaca actactctgc
      421 cactttcgca gtctatcctt cgaaagcgag aaatgaaaac tttcccgctc tgaaactcca
      481 tgggaacccc agttggctca aatcccagaa cacgaagagt ctgattggcg cgatccaggg
      541 agcgttcctc gataagaaga tcgatgtcca aggtaccacg aggcatagcg taaacagcca
      601 tcgccagacc gccgcaaagc gcgtagtcaa tcccttcttc gtcaaacttt gctgcaataa
      661 gccgtaaact ctcaacaaga tccatccaac ggcatctcca tatcacaagt tgatcactag
      721 gcttctatgc aagcgcccgc caaccaatat cctttcggta gtatacccca tcaaagcgaa
      781 ttctgctgac tgcttcatag gccttgcatt gcgccgacct catgtctgaa cccatgccaa
      841 caacgttcaa tactctgcca cctgacgtga tgacgttccc atctttgaga gccgttccgg
      901 catggaagac aacaacgtct gacatctgag cgacttgcga cagaccggag atgggtctgc
      961 ctttgggata tgagccggga tagccttcag aagccaggac tattgtcaac gcggccccgc
     1021 ctcgccatcg caaggaatgg gaagcgagat ttccctcgat acacgcaaaa gccagatcca
     1081 caagatcgga ttccagcagt ggcagaatga cctgggtctc cggatctccg aaacgcacat
     1141 tgtattccaa gacctctggc ttcccctgat caaccatcag tccggcatac agtacgccct
     1201 tgtacggatg tccctcgctc gccatggcgc gaacagttgg caacagaatt gtgtcatgaa
     1261 cctgtttgag aaaggcctgg tcgaatcgcg gtaccggcga ataggcaccc atacctcctg
     1321 tatttggccc tgtgtcgcca tcaccaacac gcttatgatc ctgggcggaa agaagcgaaa
     1381 caaccgtctc accatctgag agcgcaagta cggaaacctc ttcccctttc agtttctcct
     1441 caatgacaac ctgacgaccc gcttcaccaa cgattcggtc aaccatcaca tcatgaagcg
     1501 cccgctccgc ctcgtgctga ctgtcgggaa caaaaacacc tttgcccgca gccaacccat
     1561 ccgctttgat gaccatctcc ctgccaccac ggtattgttc aagaagaaag catcttgctg
     1621 gttcatactc atcaaaaatg ccaaaagcgg ctgtcggaat tccatgtcgc ttcatgatct
     1681 ccttagcaaa ggacttgctt ccttccagcc gggcagcagc accactgggg ccaaaggcgg
     1741 ggatcccttt cttcgcaaga gcatcaacaa ccccttcact cagaggcgct tcgggaccga
     1801 caaccaccag atccactttc tcagcaaccg ccgaatcaca gatggcagtg acattccccg
     1861 agtcaacagc aagatttctg ccgacctgag ctgttcccgc atttcctgga gcaaccagta
     1921 cttccttgac tctgctggac gaagcaagtt tccacgccag acagtgttca cgaccgccac
     1981 tgccaaccac caagactttc atcccattct cctgttctgc atcggctg
//
LOCUS       DAOQUC010000785         2024 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_71062_length_2024_cov_3.614017, whole genome shotgun sequence.
ACCESSION   DAOQUC010000785 DAOQUC010000000
VERSION     DAOQUC010000785.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71062_length_2024_cov_3.614017"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1359)
                     /locus_tag="PKH07_12605"
     CDS             complement(<1..1359)
                     /locus_tag="PKH07_12605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35823.1"
                     /translation="MRTLLVLHRLLTYVEAGLIFAVCVFFALAIGIALQIAFSPPRWV
                     AIAFFVTEIAALGVVIRLIVLPQFRKGSSESLEFALHIEQSLPDLQDRLASAIGLGNA
                     ALAIPENAPAGSVRGDPRIFSRDLLNALYLDAKHSVSRHTLSRVLLKRRAMILTSILL
                     AGLALSTQTHRWTPYTFADMINIYLNPLLKSPFTQRARYVVEPGDTEIYSGDTVRIRV
                     TLLEDSGDRPVIAFRTGTEDWDEAALEPGAQGREFSYSFSKVARSMQYQVSLSGDTSS
                     IYKIIVVSRPRLTQIEAVYKYPDYLKKSTVMGEQGKGDLLAPWGSRVQIRTQFDKALS
                     SARMDVFHGQLTLDTSASDESVESDDSVSPTVSAELLTGKPTDTYLLRAEGDVWTGDL
                     TLRYPGYYRLTAVERDFGKESEPIVYRISIQPNHLPQIDIYSPGGDVSGRDRNELQLV
                     FSV"
     gene            complement(1385..1828)
                     /locus_tag="PKH07_12610"
     CDS             complement(1385..1828)
                     /locus_tag="PKH07_12610"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HNT35824.1"
                     /translation="MAAEKETSPCLRCKPAKCCMYITLQVQTPKKLVDFEEMLWHIAH
                     QHVEYFVQKRHWFIMVHNPCRFNDAEGRCQIYHTRPTICRDHSTENCEFETGCDFDLH
                     ITSYEEMLEYASKRFPSLKERINDPSLLWENREQGNGKHSQRRKR"
     gene            complement(1840..>2024)
                     /locus_tag="PKH07_12615"
     CDS             complement(1840..>2024)
                     /locus_tag="PKH07_12615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35825.1"
                     /translation="PFHEVLSLMQDKSVEYYYYVPNERNHPILSRLGIHQHLSVHFEE
                     LYRAGDRVLYRIKSTP"
BASE COUNT          512 a          516 c          511 g          485 t
ORIGIN      
        1 caccgaaaag accagctgca actcattcct atcccgaccg gaaacgtctc caccaggaga
       61 atagatgtcg atctgtggca gatggttggg ctgaatactg atccgataga caatcggctc
      121 actctccttg ccaaaatctc gttctactgc tgtcagtcgg taatatccag gatacctaag
      181 tgtcagatcc cccgtccaaa cgtcaccttc cgcccgaagc aaataggtgt ccgtaggctt
      241 ccccgtgagg agctctgctg acacggtggg agataccgaa tcgtcactct caacagattc
      301 atctgacgct gatgtatcca gagttagctg tccatggaat acgtccatcc ttgccgatga
      361 gagcgccttg tcaaattgcg ttctaatctg cacgcgagaa ccccatggcg caagcaagtc
      421 accctttccc tgttctccca tcacggtact cttcttgaga tagtcaggat atttgtagac
      481 ggcttcaatc tgtgtcagac gaggacggga gacaacgatg atcttgtaga tggacgacgt
      541 gtcacccgag agagaaacct ggtattgcat ggagcgagcg actttgctga aggagtaact
      601 gaattccctt ccctgtgctc caggttccag agcggcttca tcccagtctt cagtaccggt
      661 gcggaatgca atgacaggtc gatcgccaga gtcttccaac aaagtaaccc tgatgcgaac
      721 cgtgtcacca gagtagatct cagtatctcc aggctcaacg acatatctcg cacgttgtgt
      781 gaaggggctt ttcagaagcg ggtttagata gatgttgatc atatccgcaa aagtatatgg
      841 agtccagcga tgcgtctgcg tcgagagggc cagccctgca agcaagatcg aagttagaat
      901 catcgcgcgg cgcttcagca gaactcttga gagagtgtgc ctggagacgg aatgctttgc
      961 atcgagatag agtgcgttga ggagatctcg agagaagata cgcggatcac cgcgaaccga
     1021 tcctgcaggg gcattctccg gtatcgcaag ggcagcattt cccagtccga tggcacttgc
     1081 aaggcgatcc tgaaggtctg gaaggctctg ttcgatatgc agtgcgaact caagggattc
     1141 agaactgcct ttccgaaatt gcggcaggac gatcagtctg atcacaactc ccaatgctgc
     1201 aatctcagtt acaaagaacg ctattgccac ccaacgaggt ggggaaaagg ctatctgcag
     1261 ggcaattcct atggccagag caaagaagac acaaaccgca aaaatgagtc cagcttcaac
     1321 atacgtcaaa aggcgatgaa gtacgagcag agtcctcacc tttcgctgta gctgactgag
     1381 aaccctaacg ctttctgcgc tgagaatgct ttccatttcc ctgctctcga ttctcccaga
     1441 gaaggctcgg atcattgata cgttccttca ggctgggaaa cctcttggat gcgtactcaa
     1501 gcatctcctc gtagcttgtg atgtgaagat caaagtcgca tccagtctca aactcgcaat
     1561 tctctgtgct atggtcacgg cagatagtgg ggcgcgtgtg gtagatctgg caacgccctt
     1621 cggcatcgtt gaagcgacag ggattatgca ccatgataaa ccaatgacgt ttctgaacaa
     1681 aatactcgac atgttggtgt gcgatgtgcc agagcatctc ctcgaaatcc acaagtttct
     1741 ttggtgtctg cacctgcagt gtaatgtaca tacagcattt tgcaggcttg catcgaaggc
     1801 aaggggatgt ttctttttcg gctgccaatg acttcactcc tatggcgttg acttgatcct
     1861 gtatagcacc cgatcgcccg ctcggtaaag ctcttcaaaa tgcactgaga gatgctggtg
     1921 aattccaagg cgcgatagaa tcggatgatt tcgctcattc ggtacatagt agtagtattc
     1981 aactgactta tcctgcatga gtgacagcac ctcatgaaag ggct
//
LOCUS       DAOQUC010000786         2022 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_71198_length_2022_cov_3.027961, whole genome shotgun sequence.
ACCESSION   DAOQUC010000786 DAOQUC010000000
VERSION     DAOQUC010000786.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2022
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71198_length_2022_cov_3.027961"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="PKH07_12620"
     CDS             complement(<1..287)
                     /locus_tag="PKH07_12620"
                     /inference="COORDINATES: protein motif:HMM:NF014166.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HNT35826.1"
                     /translation="MIVLGVDPGLSAMGYGVVKAEGGAHEALDWGVIHTQAKTTLSSR
                     LLVLYTGISAVINQYQPDIMACEGLIFAQNVQSALALGQARAAGVNQEGEQR"
     gene            complement(284..1033)
                     /locus_tag="PKH07_12625"
     CDS             complement(284..1033)
                     /locus_tag="PKH07_12625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014809278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNT35827.1"
                     /translation="MSGHSKWSTIKHKKAKTDAVRGKLFSKLIKEITVAAKHGGGDLD
                     SNPRLRTAVLTAKAANMPKSNIEQAIKKGTGELPGVNYEEHTYEGYGPGGAALFIEVL
                     TDNKNRTTAEIRYVLDRRGGNLGTDGCVAWLFERRGLVTIEAENKDEDAVLDDALELG
                     AEDVSREGNIYEIYTAPADVAEIRQGLETKGYTVDESKISMIPKTTQKLERKEAEQML
                     KLMESLEDHDDVQNVFSNFDIADDILEEMSA"
     gene            complement(1160..>2022)
                     /gene="fusA"
                     /locus_tag="PKH07_12630"
     CDS             complement(1160..>2022)
                     /gene="fusA"
                     /locus_tag="PKH07_12630"
                     /inference="COORDINATES: protein motif:HMM:NF015706.2"
                     /note="EF-G; promotes GTP-dependent translocation of the
                     ribosome during translation; many organisms have multiple
                     copies of this gene; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNT35828.1"
                     /translation="IYCLTKSDEEKLSTALGRIVEEDPSMRMFRDPVTHETVLHGTGE
                     MQVDVVTSRLKKQFNVQVELKVPKVAYKETITGAGEGSHRHKKQSGGRGQFAEVHLRL
                     RPRGRGEGYNFVNSVVGGTIPTKFIPAVEKGIKEALLAGILTGCPVEDVEVDVFFGKD
                     HPVDSSEMAFKIAAGMAFREVALKSNPILLEPIVRMEITVPEEHMGDVIGDMNSKRGR
                     ILGMDTVKGRSTIRATAPLSEVLRYSIDLKSIARGRGSYTMELDTYEPVPYEKTQQII
                     ADVKAAKEAE"
BASE COUNT          399 a          609 c          498 g          516 t
ORIGIN      
        1 cgctgctccc cttcctggtt gaccccggcg gcacgcgcct gccccagggc aagagcactc
       61 tgaacgttct gtgcaaaaat caagccttcg catgccataa tgtcaggttg gtactgattg
      121 ataaccgcgg aaatgcctgt gtacaaaaca agaagacgac tcgaaagagt cgtcttcgcc
      181 tgcgtatgaa ttactcccca gtccagagcc tcgtgcgctc caccctcagc cttgacgacg
      241 ccataaccca tggcgctcag tccggggtct actccgagca caatcacgca ctcatctcct
      301 caaggatgtc gtcggcgatg tcgaagtttg agaagacatt ctgaacatcg tcatgatcct
      361 caagggattc catcaacttg agcatctgtt cggcttcctt gcgctcaagt ttctgagtcg
      421 tcttggggat catgctgatc ttggattcat ccaccgtgta tcctttggtt tccagaccct
      481 gtcggatctc tgcgacgtcc gccggtgccg tgtagatctc gtagatgttg ccttcgcggg
      541 acacgtcttc agcaccgagt tccagcgcat catcgagaac tgcgtcctcg tctttattct
      601 ccgcctcgat tgtcacaagt ccccggcgtt caaacaacca agccacacaa ccatcggttc
      661 cgaggtttcc accgcgccgg tccagaacat atcgaatctc ggctgttgtt cgattcttgt
      721 tgtcggtgag cacctcgatg aagagagcag cccctccggg gccatatcct tcgtaggtgt
      781 gctcttcata gttgacgccc gggagttctc ctgtaccctt cttgattgcc tgttcgatat
      841 tgctcttggg catattcgct gctttggcgg taagaacagc cgttcgcagt cgggggttgg
      901 agtccaaatc gccaccgcca tgttttgcgg ctactgtgat ttccttgatg agtttcgaga
      961 acagctttcc tcgaaccgca tccgtcttgg ctttcttatg cttaatcgtt gaccacttag
     1021 aatgtcctga cattcaggca cctccacaat tctcctgaga agaaggcctt ccggagtctc
     1081 gtatccactc agggcaagaa tcggccagag agcgggagtc gccctggact ctccggcaaa
     1141 cctgccgacg cacaggccct tattcggctt ccttcgcggc tttgacgtcc gcgatgatct
     1201 gctgcgtctt ctcgtacgga accggctcat aggtatccag ttccatggtg taacttcctc
     1261 gacctcgcgc gatggacttc aggtctatgg aataccgaag cacttccgac aggggagccg
     1321 tggctcggat cgtcgagcgt cccttgaccg tatccattcc gagaatcctg cctcgcttgg
     1381 agttcatgtc tccgatcaca tcgcccatgt gttcctcagg aaccgtaatc tccatgcgga
     1441 caatgggctc cagcaggatc ggattgctct tcagggcaac ctcgcggaat gccatacccg
     1501 cggcgatctt gaatgccatt tccgaggaat ccaccggatg gtcttttcca aagaagacat
     1561 ccacttctac atcctcaacg gggcagcccg taaggattcc agccagcaat gcctctttga
     1621 tgcccttctc aacggcaggg ataaacttgg tggggattgt tccacccaca acactgttga
     1681 caaagttgta tccttcgccc cgtccgcgtg gtcgaagtcg caaatgaact tcggcaaact
     1741 gaccacgacc accggactgc ttcttatgtc ggtggctgcc ttcacccgca ccagtgatcg
     1801 tttctttgta ggcgaccttg ggcactttga gttcgacttg gacgttgaac tgcttcttca
     1861 gtcggctggt caccacatcc acctgcatct ctcccgtgcc gtgcaagacc gtttcatggg
     1921 tcaccgggtc gcgaaacatg cgcatgcttg gatcttcttc aacaattcga ccgagggcgg
     1981 tgctgagttt ctcctcatca ctcttggtca ggcagtagat tg
//
LOCUS       DAOQUC010000787         2022 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_71215_length_2022_cov_2.209456, whole genome shotgun sequence.
ACCESSION   DAOQUC010000787 DAOQUC010000000
VERSION     DAOQUC010000787.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2022
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71215_length_2022_cov_2.209456"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..539)
                     /locus_tag="PKH07_12635"
     CDS             complement(<1..539)
                     /locus_tag="PKH07_12635"
                     /inference="COORDINATES: protein motif:HMM:NF016450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HNT35829.1"
                     /translation="MNLVQYGYRQRDTYARIPSLIEMPDLIETQQKSYRDFLQAGVLP
                     DKRRSEGIQGAFETVFPIKGRDGSSLEFVNYSFGRPKYNVQECVDREMTYAAPLRIRV
                     RLVVKLPGDKEDTSELVDIREEDIYLGELPMMTQKGTFIINGAERVVVSQLHRSPGVY
                     YSFNRAKKMYSAKIVPYRGA"
     gene            complement(891..1706)
                     /locus_tag="PKH07_12640"
     CDS             complement(891..1706)
                     /locus_tag="PKH07_12640"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNT35830.1"
                     /translation="MKIETLNRSAEARQVGIWWCLLFGTILVVSYGLSLLLPYVSEMS
                     FRMDPAQTMREGFKLIAQRDFDSAEALMTAVLQQDPEKHQAYHGRAMIYREQGRLDEA
                     LSAVEAALQRRPEEPSYVRTKIGVLADLGRYDEAIQICGQMLERKVKLDVFQLEMGNL
                     YYRQQKWDQALMPLKTALALNPDNTEAERLLAYTYMCTNQHAEAVVLWERLKTKDPGR
                     AEYYYYLGLCLLDAGLDKQEALRNLKSSARLDSENFDDKVAFQIRHRGLDREP"
     gene            complement(1758..>2022)
                     /locus_tag="PKH07_12645"
     CDS             complement(1758..>2022)
                     /locus_tag="PKH07_12645"
                     /inference="COORDINATES: protein motif:HMM:NF013401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HNT35831.1"
                     /translation="FHDIKPQAPAHILIIPKQHIARISELQNCHISLMGEMLLLAATI
                     ARQQGFEQDGYRLVLNCGENAGQEVFHIHLHLLAGRKFAWPPG"
BASE COUNT          518 a          513 c          484 g          507 t
ORIGIN      
        1 gcgccacgat acggaacgat cttggcagaa tacatttttt tggcgcggtt gaaactgtaa
       61 taaacgccgg gtgagcgatg cagctgactt acaacgacgc gctcagcgcc attgatgatg
      121 aaagtgcctt tctgtgtcat catgggcagt tcgccaaggt agatatcctc ttcacggata
      181 tccacaagtt cggaagtgtc ttccttgtca ccaggaagct tgaccactag gcggactctg
      241 attcgcagag gagccgcgta ggtcatctca cgatccacgc attcctgcac attgtacttg
      301 ggacgcccaa aactgtagtt gacaaactcc agggaagaac catccctgcc cttaatgggg
      361 aaaaccgtct cgaaggcacc ttggatgcct tccgatcttc gtttgtccgg gagaacacct
      421 gcttgcagga aatccctgta ggacttctgc tgtgtctcaa tcaggtccgg catctcaatg
      481 agactgggga ttctggcgta agtatcacgc tggcggtatc catactggac aagattcatt
      541 cggaagaaac tcctttcccc tgagacagaa tcatatctgc cttcagcaaa tacctataaa
      601 ccaaggaaac taaagactcg accggttgac tgatcatcaa gcgtttcgca ggaatcgttt
      661 ttgcaccata ccttatgcaa taacgtactg taaccagaag taaaggaata tacactcgat
      721 acagggtttg tcaattaaaa aatcctgtac tctcaaaaaa atacttcaat catgatgatt
      781 acggatgaga tcagcaaaag tcacgccaaa aattacactt ggggtcaaac ggctcaaaaa
      841 aacgcccaca atcccagtct cttgacgcga gggaaacggg ctctgcggct ctatggctct
      901 ctatcaaggc ctcgatgacg gatctgaaag gcaaccttgt cgtcgaagtt ctccgaatcc
      961 agacgtgcgg aggacttcag gtttctgagg gcttcctgtt tgtccaatcc cgcgtcaagc
     1021 aggcacagac caaggtaata gtagtattca gcccttcctg gatccttggt tttgagtctc
     1081 tcccagagca cgacggcttc ggcatgctga tttgtgcaca tatatgtgta agcaagtagc
     1141 ctttcagcct cagtattatc aggattgagc gccagagcag tcttgagtgg cataagtgct
     1201 tgatcccact tctgttgacg atagtataga ttgcccatct cgagttgaaa aacgtccagt
     1261 ttcacctttc gctcaagcat ttggccgcag atttggatag cttcgtcgta acgtcccaag
     1321 tccgccaaga ctccaatttt ggttcggaca taagaaggtt cttcaggtcg cctctgcagg
     1381 gctgcctcca ccgcgctcaa ggcttcatcc agtcttccct gctcacggta aatcattgcc
     1441 cttccgtgat acgcctggtg tttctcgggg tcttgctgga gcacagcggt catcaatgcc
     1501 tcagcagagt caaaatcccg ttgtgcaatg agtttgaagc cctctctcat tgtttgtgcc
     1561 ggatccattc ggaaagacat ctcggacaca tagggaagaa gaagtgagag tccgtaagaa
     1621 accaccagga tcgttccaaa cagaagacac caccagatgc cgacctgtct tgcctcagct
     1681 gaacggttaa gagtctcgat cttcaattca ggcacccaat cacaagtgat ctcaggcagt
     1741 ctggatacat ggagagtcta gcctggtggc catgcaaact ttctgcccgc aagaaggtgt
     1801 agatgaatgt gaaatacttc ttgtcctgca ttttcaccac agttcaagac cagtcggtaa
     1861 ccatcctgct cgaatccttg ctggcgggca atggtggcgg cgaggagcaa catctcaccc
     1921 atcagagaga tgtggcaatt ctgcagttct gatatccttg caatgtgctg ctttgggatg
     1981 ataagaatat gtgcaggtgc ctgtggcttg atgtcatgaa ag
//
LOCUS       DAOQUC010000788         2020 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_71284_length_2020_cov_3.319084, whole genome shotgun sequence.
ACCESSION   DAOQUC010000788 DAOQUC010000000
VERSION     DAOQUC010000788.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71284_length_2020_cov_3.319084"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1139)
                     /locus_tag="PKH07_12650"
     CDS             complement(<1..1139)
                     /locus_tag="PKH07_12650"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNT35832.1"
                     /translation="MARESKPNFVFVFADDWGWGDLGCYGHPFVKTPNLDRMASEGIL
                     FSQFYVCSGVCSPSRAAAVTGLFPSRLAIHGHFANHQQNDARAMPDWLDPQLQTYMHL
                     LRQSGYVIGHFGKWHLGSGLGAPEPFAYGIDECRVNAGNGPQLDFPSAYGSHRSMQGY
                     HNGKEIPWPSKHIRWKSSQVIVDESLSFIERHQHRPFLVNVWLNDTHATLDPTPEQLE
                     VYSDLFPEELQEKYVGAPAIYYAAVTDSDRHIGRLLDRLDQLGLRDNTVVIFSADNGP
                     EDIVIRNASHSGVGSPGPFRGRKRSLYEGGIRTPFILRWPAGGARPDTVDDATSLCAV
                     DLLPTFCQLAEVALPGGITLDGEDMSAAFRGTPVQRTKPLLWEWR"
     gene            1318..1977
                     /gene="tmk"
                     /locus_tag="PKH07_12655"
     CDS             1318..1977
                     /gene="tmk"
                     /locus_tag="PKH07_12655"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HNT35833.1"
                     /translation="MNQGKLITFEGIEGSGKTTQMDRLCGLLENQGCRIQRFREPGGT
                     ALGERIRDLLLAPDAEVERLTELLLFQAARRQLVSTRLEPALAEGCVVLLDRFYDATM
                     AYQGYGRGIPLDLIRQMTRVACGKLVPDLTILLDVDPKQGLNRCLLREGREYAGADRI
                     ESSGLDFLERVRCGYLELAAQEPDRFVVVRVTENIEKTFLQVVHSLQVRKVLPIDIRK
                     S"
BASE COUNT          494 a          504 c          561 g          461 t
ORIGIN      
        1 aatcgccact cccagagaag cggtttggtt ctctggacag gggttcctcg aaaagccgca
       61 gacatatcct ctccatccag agtgatacca ccaggaagag cgacctctgc cagttggcag
      121 aacgttggca ggagatccac tgcacacagt gaagttgcgt catccacagt gtcaggccgt
      181 gcccccccgg caggccatct gagtataaag ggggtacgga tgcccccttc atagagactg
      241 cgttttcttc cgcgaaatgg gcctgggctt ccgacgccac tatgggaagc attgcgtatg
      301 acaatatctt ccggcccatt gtccgcagag aagattacta ctgtgttgtc gcgaagcccc
      361 agttgatcga gccgatcgag caaacgtccg atgtgacggt ctgaatccgt gacagcagca
      421 tagtaaattg cgggtgcacc cacatacttt tcctgcagtt cttcgggaaa caagtctgag
      481 tagacctcaa gttgttctgg cgtaggatcc aacgtggcat gcgtgtcgtt aagccagaca
      541 tttacgagaa agggacggtg ctgatggcgt tcgatgaatg acagggactc atccacaatg
      601 acctgcgatg acttccaacg tatgtgcttg cttggccagg ggatttcctt gccgttatga
      661 taaccctgca tgctccgatg actcccgtac gcagaaggga aatccaactg gggaccattg
      721 cccgcattga cacgacactc atcaatgccg taggcaaaag gttcaggggc ccccagccca
      781 cttccaagat gccacttgcc gaagtgtccg atgacatagc cactttgtct caacagatgc
      841 atgtaggtct gcagttgcgg atcgagccaa tccggcattg ctcttgcgtc attctgctgg
      901 tgattggcga aatgaccatg tattgcgaga cgggatggga acagtcctgt aactgctgca
      961 gcgcgacttg gagaacacac tccagagcag acatagaact gagagaaaag aatcccttcg
     1021 gaagccatcc gatccaggtt tggagttttc acaaaaggat gaccatagca tcccaggtct
     1081 ccccatcccc agtcatccgc aaaaacaaag acaaagttcg gttttgattc tctcgccatg
     1141 tgctgatcct gacatacagg gccacaaact cattgaccgt cacctgtaga atggcatctt
     1201 tggcttttcc tggcaaacgg aagtctttga caataccgag acagcgaatc taagattcac
     1261 tcaagatata tggaatcaca ggagccgaca aagattttgg cgccaaatgg gagagagatg
     1321 aatcaaggca aactgatcac gttcgagggc atagaaggtt cgggcaagac aacccagatg
     1381 gatcgtcttt gtggactgct ggaaaatcag ggatgcagga tccagcggtt tcgggagccg
     1441 ggaggaactg ctctcggtga gcggattcgg gacttgttgc ttgctcccga tgcagaggtc
     1501 gaaaggctga ccgagctttt gctctttcag gcggcacggc gtcaactggt ttccacaaga
     1561 ctcgaacccg cacttgcgga gggttgtgtt gtactcctgg atcgtttcta cgatgccacg
     1621 atggcctatc agggctatgg tcgaggaatc cctcttgacc tcattcgaca aatgacccgg
     1681 gtcgcttgtg ggaaactggt tccagacctc acgatactgt tggacgtaga tccgaagcag
     1741 ggcttgaatc ggtgtctact gcgtgagggg agagaatatg ctggagcgga tcggatcgag
     1801 tcatccggtc ttgatttcct ggagcgggtt cgatgcgggt acctcgaatt ggctgcacag
     1861 gagcccgatc ggtttgtggt ggtgcgcgtt actgaaaata tcgagaagac gtttttgcag
     1921 gtagttcatt ccttgcaagt gaggaaggtg ctgccgatag acatccggaa gtcttgatgc
     1981 ccccaggaaa gcaggaagtt tcatagaatg gaggacttaa
//
LOCUS       DAOQUC010000789         2018 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_71391_length_2018_cov_2.985227, whole genome shotgun sequence.
ACCESSION   DAOQUC010000789 DAOQUC010000000
VERSION     DAOQUC010000789.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71391_length_2018_cov_2.985227"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /gene="rnpB"
                     /locus_tag="PKH07_12660"
     ncRNA           complement(<1..185)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PKH07_12660"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            484..1890
                     /locus_tag="PKH07_12665"
     CDS             484..1890
                     /locus_tag="PKH07_12665"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNT35834.1"
                     /translation="MMDQQNASTEHEYGLSRRRFLSAAGVGAAGMALGCRTLGSMREH
                     AGKRKPNVIVIFSDDHGYGSMSCMPNWNIPTPNIDSIAANGVRFTDGYVTCPICGPSR
                     AGLLTGRYQQRFGFEENSGPIPKAPANYGLPLDEKTLADYMKELGYVTGMVGKWHLGL
                     KPEYHPQKRGFDEFFGFLHGAHSYIDAGFQTDNPILRGTERVDEKAYLTDAFAREAVS
                     FIDRHQKEPFFLYVPFNAVHSPNEAPGRFKGKFPNLQPPVLRTWAGMLAAMDEAVGKI
                     LDKVRETGLEEETLIFFLGDNGGVLVGGVEANYPLRGRKAEMYEGGIRIPFLVQWKGH
                     LPNNTLYEKPASSLDILPTAVALAGGKPASNTEGVNLLPYLTGENTGTPHERLFWRWF
                     EKSAARVGDWKLVREGDGSQQLFNLSTDLGETTDLSASRPDKLKEIQDAYDEWNARNV
                     APKWRDSRRKTGQEYLGI"
BASE COUNT          458 a          550 c          556 g          454 t
ORIGIN      
        1 ctgccggtgc gctcttaccg caccatttca cccttaccca cgttcgaaaa cgcgggcggt
       61 atgttttctg ttgcactttc cgtaggctta cgcctcctgg gtgttaccca gcaccctgct
      121 ctgtgaagcc cggactttcc tcggacgcgc tttgggagcg cggacgcgat cgcccgaccc
      181 accagccatc tcttctaacg gatcattgta cagaaaacaa cgaagtgttc cactgaacac
      241 acgcaagaga aggagcagcc tctgaaagta tcggtttaca gtaacacacc gcatggggta
      301 gagtcatgag tgcagtacat tgggaacagg aatctggctc aatcttgacg gactcctggt
      361 gacacggagt ccgagaagga cttgtccttt attgagcaag cgtccgaagg agtgggcata
      421 gcccacgcgc ccgcaggcgc tggcctgcac ggaatgaccg gttctgctat ctctcggagg
      481 aaaatgatgg atcaacagaa tgcttcaact gagcacgagt acggtttgtc tcggcgtcgg
      541 tttctcagcg ctgcgggcgt tggagctgct gggatggctc tcggatgtcg aacgttgggt
      601 tcgatgcgtg aacatgccgg gaagcgcaaa cccaacgtca tcgtcatctt ctcggacgac
      661 catggctatg gttcgatgag ttgcatgccg aactggaaca tccccacacc gaacattgac
      721 tccatcgctg ccaatggcgt tcgattcacc gatggctatg tgacttgtcc aatctgcggt
      781 ccgtcccgag cagggctttt gactggacgc tatcaacaac gttttggttt tgaggagaat
      841 tcaggtccca tcccaaaagc acctgcaaac tacggccttc cactcgatga aaagactctt
      901 gccgattata tgaaggaact gggttacgtg acggggatgg ttggcaaatg gcatcttggg
      961 ctgaaacctg aatatcatcc tcagaagcgt ggatttgacg agttcttcgg attcctgcat
     1021 ggagcccatt cctatattga cgccggtttt cagacggaca atccgatcct tcgcggcacg
     1081 gagcgggtgg acgagaaggc atatctgacg gatgcatttg cgcgagaagc cgtatcgttt
     1141 attgaccggc accagaaaga acccttcttt ctttacgttc ccttcaatgc cgttcacagt
     1201 ccgaatgagg ctcccgggcg tttcaaggga aaattcccaa accttcagcc acctgtactc
     1261 agaacctggg cggggatgct cgcggccatg gatgaagcgg tcggcaagat tcttgacaag
     1321 gttcgtgaga ccgggttgga agaggagacg ctgatcttct tcctgggcga caacggcggc
     1381 gttcttgtcg gtggtgtcga ggcaaactat cctcttcgcg ggcgcaaagc cgaaatgtac
     1441 gaaggcggca ttcgcatacc gtttctcgtt cagtggaagg gtcatctgcc aaacaacacc
     1501 ctctatgaga aaccggccag ctctttggat attctcccaa ctgccgttgc cttagcaggc
     1561 gggaaacctg catccaatac cgaaggggtg aatctgttgc catacctcac aggtgagaac
     1621 actggcacgc cccatgagcg gctcttctgg cgctggtttg aaaaatctgc ggcgcgagtc
     1681 ggagactgga aactcgttcg agaaggcgac ggttcacagc aactcttcaa cctgtcaact
     1741 gacctcggcg agacaactga tctgtcagca tcgcgtcccg acaaactcaa agagatccag
     1801 gacgcttacg acgaatggaa tgccagaaac gttgcgccca aatggcgcga cagtcgaaga
     1861 aagaccggtc aggaatacct tgggatataa cgtcatcgta ggcaggttgg cacctgcgga
     1921 cacgttccgg gtgccccctc tcttctcatg ctccgactga ctcctctggg tgccaactct
     1981 cagggtcgcg aagcggttct gagggctgct gatttatc
//
LOCUS       DAOQUC010000790         2018 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_71397_length_2018_cov_2.575650, whole genome shotgun sequence.
ACCESSION   DAOQUC010000790 DAOQUC010000000
VERSION     DAOQUC010000790.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71397_length_2018_cov_2.575650"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1550
                     /locus_tag="PKH07_12670"
     CDS             <1..1550
                     /locus_tag="PKH07_12670"
                     /inference="COORDINATES: protein motif:HMM:NF037202.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35835.1"
                     /translation="HHHAQLTGSSIVGYVTNNSFLDGLIHRAMRKHLLDDFGRIVILD
                     LRGSTRRGDTADANISDANVFDIQQGVTITFLVRGSSHSISTSVEHGVVMGCRESKYA
                     TLSSLSIEFTTFSNIQPEAPFFFFAPKDFGLKVEYNRFLGWDDLFHEFKLGFMSGNDQ
                     LLIDTDAGVLRRRIQRVLDPSISEKKAAVEFSIQENYPIGRMRRARFDKRKVIKCGYR
                     PFDERFTYYDDHVMERALYSINRHLMRENLAICVSRQLSRLPYRHVFVTNKVTEMSLV
                     SNKTKEANRVVPLYLYRSEDAAILESVTNEERQPNIKREAMTLVGGILGLRWLEDGQG
                     DLASSIGPESIFFYTYGVLHSPIFRKRYAPFLEIDFPRVPLPGSLDLFRALANLGGEL
                     VALHLMESPKLDKHITAFQGDRSREVEKVSYSKETVWIDKILKTGFKGVPEEVWNFHI
                     GGYQVCQKWLKDRKGRTLSEEDITHYHRIVVALKETIRLMAEIDVVIEKHGGWPDAFA
                     TSVKKDS"
     gene            complement(1759..>2018)
                     /locus_tag="PKH07_12675"
     CDS             complement(1759..>2018)
                     /locus_tag="PKH07_12675"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNT35836.1"
                     /translation="GIDPCELKAQFGEHLCFHGAISIQRILAQGTTDDVARETRRLID
                     ILGMEGGYILAPTHWIQSGTPIENVLTLYQVAQDYSRQVYR"
BASE COUNT          501 a          477 c          550 g          490 t
ORIGIN      
        1 ctcatcacca cgctcaactc actggttcgt ctatcgttgg ttacgttacg aacaactctt
       61 ttctagatgg tctcattcac agagcgatgc gaaagcactt gcttgacgat ttcggcagaa
      121 tagtgattct ggatctgaga ggaagcacga gaagagggga tacggcagat gccaacatct
      181 ctgacgcgaa cgtctttgac attcaacagg gcgtcacaat tacttttcta gtacgaggta
      241 gctcacatag cataagtaca tccgtagagc atggagtcgt catggggtgt agagaaagca
      301 agtacgccac cctctcgtct ctatcgatag aatttaccac tttcagtaat atccaaccgg
      361 aggcgccgtt cttctttttt gccccgaagg attttggcct gaaagtcgaa tacaaccggt
      421 tcctgggatg ggatgacctg tttcacgagt ttaagttggg gttcatgagc ggcaatgacc
      481 agttgttgat tgataccgat gccggagtgt tgcgacggcg tattcagaga gttctggatc
      541 cgtcgatcag cgagaagaag gctgctgtcg agttcagtat ccaggagaat tacccaattg
      601 gccggatgag aagagctaga tttgacaaac ggaaagtaat caagtgcggc tatagaccct
      661 ttgatgagcg tttcacatat tacgacgacc acgttatgga gcgggctctc tattcgatca
      721 atcgacatct catgcgtgag aatctagcga tctgtgtcag ccgtcagttg agtcgtctcc
      781 cctataggca tgtctttgtg accaacaagg taacagagat gagcctcgtc tcaaacaaga
      841 cgaaagaggc aaacagggtt gtacccctgt atctgtaccg ctctgaagat gccgccattt
      901 tggagtcggt aacgaacgag gaaagacagc cgaatatcaa gagggaagca atgaccctgg
      961 ttggaggcat tctggggttg aggtggcttg aagacgggca aggggatttg gcatcatcga
     1021 ttgggccgga gagcatcttt ttctacacat acggtgttct ccattcaccc atttttcgca
     1081 agcgatatgc gccttttctt gaaatcgatt tcccgcgcgt tccgctacct gggagcctcg
     1141 atctgttccg tgctttggca aatctgggtg gggagctggt tgccctgcac ctgatggaat
     1201 ctccgaaatt ggacaagcac atcacggcgt ttcagggcga caggagccgt gaggtcgaga
     1261 aagtgtctta ttcaaaagag acggtctgga tcgataagat attgaagacg ggcttcaaag
     1321 gcgtacccga agaggtctgg aactttcata tcggtgggta tcaggtctgt cagaaatggc
     1381 ttaaggatcg caaagggaga accctctccg aagaggacat cacgcactac cacaggatcg
     1441 tggtcgccct caaggaaacc atccgcctta tggcggaaat agacgtagtc atcgaaaagc
     1501 acggcgggtg gcctgatgcc ttcgccactt cagtgaagaa ggactcctga ctccgtaatt
     1561 gtgatgctga catcttgcct gcttcttatc tcagaacgga agcaggctgg aagcccgcac
     1621 cacaacaacg gagtatgggg attgtgatgc aggcatcttg cctgctgctt atctcagatc
     1681 ggaagcaggc tggaagcccg caccacaaca gaatgggact caccctatgc gcttcactga
     1741 aaggcatgcc tctcttttct accgatacac ctgccggctg tagtcttgtg cgacttggta
     1801 gagggtgaga acgttctcaa tgggcgttcc ggattggatc cagtgggtgg gggcgagaat
     1861 atagccgcct tccataccga gaatgtcaat aagtcgtcga gtctcgcggg cgacatcgtc
     1921 ggttgttccc tgagccagaa tgcgctggat gctgatggca ccgtggaaac acagatgctc
     1981 tccaaattgt gctttcaatt cgcaaggatc aatgcctt
//
LOCUS       DAOQUC010000791         2017 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_71444_length_2017_cov_2.978593, whole genome shotgun sequence.
ACCESSION   DAOQUC010000791 DAOQUC010000000
VERSION     DAOQUC010000791.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71444_length_2017_cov_2.978593"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2017)
                     /locus_tag="PKH07_12680"
     CDS             complement(<1..>2017)
                     /locus_tag="PKH07_12680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35837.1"
                     /translation="NIDRETGLNLHIDTRIDDFTNWDFRKLEFVLSAPTWKVEMLAPF
                     LQNLSERSLPISGQGSLEGQINGEGKGSCAVAVREMSIGESFRANVEASFRDITYRRD
                     TGQLGASLRIGCKDMRLSNLEAQQASFVSDLEYSTPDGRFQVRDAVLAMDSLNASMNL
                     TGSLDGNSGELMNAGIRFEELPLEYLMALLGILTDRFGSINGTEGECFADLKMSVVRM
                     KQPPGTKMTVSGTVGLTGSSGSGYEDMLIWSDLGARWDGELESQYSIGDEPEASIRGT
                     ASLEDGEFLYDTLYHDLKVSPIDISVQSVVKKTGMIKLQSGALRIPKTLEVQAHGDVQ
                     AASPLSYDLVVNASASLDKFLPDIVASWLSGGSDRFNHASATGQTQMELAVVGDATNT
                     FLDGQIHLHAQTLDLPMLKELHMENLVAEIPFRIHRGALAKTRSSDVPLGEIRFDRLS
                     QTRMNIGRVRIPLHVQDNVLNIPQPISVSIWDGQIHLNEFQMENILDRYSKIYGRGRV
                     EDLALPLLTEWLAWPAVDGTLSCDRLEMQSEGGALKLLYPLTADVFGGLLVIEEMQWL
                     DYLERFPKLKMSMEIRQIDLGQLSSRFPIGQIRGVLTGHVRELIFQAGLPTRFDIELE
                     TESRARSQQQISVEAIKTILMVDPRTLRDQAVRQLLERTEMVYYSKIG"
BASE COUNT          480 a          583 c          487 g          467 t
ORIGIN      
        1 aaccgatttt gctgtagtac accatctcgg ttctttccag aagttgtcga actgcctgat
       61 cccgaagtgt acgaggatcc accatgagga tcgtcttgat cgcttccacg cttatctgct
      121 gttgcgatcg cgcacgactc tccgtctcca gttcgatatc gaatcgagtt gggagcccag
      181 cttgaaagat aagttccctc acatgtccgg tcagcacacc gcgaatctgg cctatcggaa
      241 agcgactgga aagttgcccc aagtcgatct gcctgatctc catggacatc ttcagcttgg
      301 gaaatctctc cagataatcc aaccattgca tctcctcgat gaccaaaagt cctccgaaaa
      361 cgtccgccgt cagcggatag aggagcttca gcgctccacc ttcagactgc atctccagtc
      421 gatcgcagct gagtgtccca tctaccgcag gccacgcaag ccactcggtc aggagaggca
      481 gagccaaatc ctcaaccctt ccccgaccgt agattttcga ataccggtcg aggatgttct
      541 ccatctgaaa ctcattcaga tgaatctgtc cgtcccatat tgagacggaa atcggctgtg
      601 ggatattcag cacgttatcc tgaacgtgga ggggaatccg aactcgccca atgttcatcc
      661 tggtctggct cagacgatca aaacggatct cacccaaagg aacatcgctg ctccgtgtct
      721 tggcaagcgc acccctgtgt atacgaaaag gaatctctgc gacgagattc tccatatgca
      781 actctttgag cattggcaag tccagggttt gtgcatggag gtgaatctgt ccgtcgagaa
      841 acgtattcgt tgcgtcgccc acgacggcca attccatttg ggtctgccct gttgcgctgg
      901 catgattgaa acggtcagaa ccgccagaga gccacgaggc aacaatgtca ggcaggaact
      961 tgtccagcga cgcacttgca ttgacaacaa ggtcatagga gagtggagaa gccgcttgga
     1021 catctccatg cgcctgaact tcgagcgtct tcggaatgcg gagggcacca gactggagtt
     1081 tgatcatgcc ggtcttcttg acgacgctct gaacggatat gtcaatcgga gagaccttca
     1141 agtcatggta cagagtgtca tagagaaact cgccatcctc aaggcttgcc gtgccccgaa
     1201 tagaagcctc cggctcatct ccaatggaat actgcgactc aagttcgccg tcccatcggg
     1261 ctccaagatc gctccagatg agcatatctt catagccaga accggaactg cccgtcaatc
     1321 caacggtccc cgaaaccgtc atctttgtcc ctggcggttg cttcatccga acaacgctca
     1381 ttttcagatc ggcgaaacat tctccctcgg tgccgttgat ggaaccaaat cggtctgtga
     1441 ggattcccaa cagcgccatg agatactcga gaggcaactc ctcaaagcgg attcccgcat
     1501 tcataagttc tccggagttg ccgtccaaac tccccgtgag attcatcgag gcattcagcg
     1561 agtccatcgc gaggactgca tcccgcacct gaaaccggcc atctggagtt gaatactcca
     1621 agtcactgac aaatgacgcc tgctgcgcct caagattgga caggcgcatg tctttgcagc
     1681 ctattcgcag agatgcacca agctgacccg tgtctcgtcg gtaagtaatg tctcggaagc
     1741 ttgcctcaac gttcgcccga aacgattccc caatgctcat ctctctgaca gcaacggcgc
     1801 aactcccctt cccctctcca ttgatctgtc cttccagact gccctggccg gaaatcggca
     1861 gagaccgttc cgagagattc tggagaaagg gagcaagcat ttccactttc caagtgggcg
     1921 ccgagaggac gaattcgagt tttctgaaat cccagttggt aaaatcatcg attcgagtgt
     1981 caatatgaag gtttagtcct gtttctctgt caatgtt
//
LOCUS       DAOQUC010000792         2014 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_71635_length_2014_cov_2.512506, whole genome shotgun sequence.
ACCESSION   DAOQUC010000792 DAOQUC010000000
VERSION     DAOQUC010000792.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71635_length_2014_cov_2.512506"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(135..1658)
                     /locus_tag="PKH07_12685"
     CDS             complement(135..1658)
                     /locus_tag="PKH07_12685"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNT35838.1"
                     /translation="MEQPYAVGNYSRLHRVLTEALNVLALPIGVLFLVDEDLHISPLQ
                     TQGIAEDRLRHHPLSLRIRDYEQTIQNWDGVVVDLPQDDLLSKWVLEISGQTDGVHYG
                     KALAIPILRSQSLLALIVALSDRSTMDIPVKFRALQLLMEGAGAAISNAIIFEKMNQK
                     ALALTCIHAMHRLMNYPITEQDLLSRIGRVILQALQVTKCSIMLINKERTLLEPRVWI
                     NLEKDEVGTAPLRSGESIPGWVWENYAPLIVNSWKDDHRFENDPASTYPSQRYLSMPM
                     IESDVLGVITVSGKEKDFTPGDREVLQYVTEQSVIALNNARLCETQQHLTLNTLKSIA
                     NVVEMKDPYKVGHTERMAECAEQIAARMGLDYESRMNLKYAALLHDAGGIGVRERDTS
                     DAAMNDIVRDMEGHLDMSVQIARSLGLSETIVKMIRHHHENFDGSGYPGGLRGQAIPL
                     GSRIIAVADIFVALTAGRGSHRTLSPQEAINFLRNLAGKRFDPEIVSLLARIALHPK"
     gene            1657..>2014
                     /locus_tag="PKH07_12690"
     CDS             1657..>2014
                     /locus_tag="PKH07_12690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005674095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNT35839.1"
                     /translation="MKPSELFPILASDLAETDRVIRQRLGSEVALINSIADYIIGAGG
                     KRMRPLLLLLFARALGYKGASNHYLLAAVVEFIHTATLLHDDVVDESALRRGRQTANA
                     AFGNAASVLVGDFLYSR"
BASE COUNT          456 a          568 c          542 g          448 t
ORIGIN      
        1 gacgagtgcg gggtcccagc caagcttgcg gaccaattca tacgccaagc catcctgctt
       61 cgattccttt gaagagaagg acacttctgt cctgagacat atatacacca acgcggggaa
      121 tcggcaaagc tgctctattt ggggtgcaag gcgattcggg cgagaaggga aacgatctcc
      181 ggatcgaagc gttttccggc gagattgcgc aaaaagttga ttgcttcctg aggggagaga
      241 gtgcggtgac tgcctctgcc ggcggtcagg gcgacaaaaa tgtccgcaac agcaatgata
      301 cgtgagccca gaggaattgc ctgacctcga agcccgcctg gatagccgct tccgtcgaag
      361 ttctcatgat gatggcgtat cattttgacg atagtctccg aaagccccag actccttgcg
      421 atctgaacag acatatcgag atggccttcc atgtctcgga cgatatcgtt catggctgcg
      481 tcgcttgtgt ctcgttctcg aaccccgatg ccgccagcgt cgtgtagaag ggctgcatat
      541 ttgagattca tccgtgactc atagtccaga cccattctcg cggcgatctg ctcagcgcac
      601 tccgccatcc tttcggtatg tccaaccttg taagggtcct tcatctcgac aacattggct
      661 attgacttga gtgtgttgag cgtcaagtgc tgttgggttt cacagagccg ggcattgtta
      721 agtgcaatga ccgactgctc ggtgacatac tgaagaactt ctctgtctcc aggggtaaaa
      781 tccttttcct tgcccgacac ggtgatgact cccagaacat ctgactcgat catgggcatg
      841 gaaagataac gctgagaggg atatgttgac gcaggatcgt tctcaaatct atgatcgtct
      901 ttccaggaat tgacaatcaa gggcgcgtag ttctcccaga cccatcccgg gatggattca
      961 ccacttcgca gcggtgccgt gcccacttca tctttctcaa ggttgatcca gactctcggt
     1021 tccagaagag tccgttcctt gttgatgagc ataatggagc acttggtgac ctgaagcgcc
     1081 tggagaatca cccgaccgat tcgggacaga agatcctgtt ccgtgatggg atagttcatc
     1141 aagcgatgca tggcgtggat gcaggtcaag gccagcgcct tttggttcat cttctcaaag
     1201 atgattgcat tgcttatagc ggcgcctgcg ccttccatga gcaattgcag ggcccggaat
     1261 tttacaggaa tatccatggt cgagcgatcc gacagggcaa cgatcagagc aagcaacgat
     1321 tgtgaacgga gaatgggaat cgccaaggcc tttccgtaat ggacgccgtc ggtctgtccg
     1381 gaaatctcga gaacccactt ggaaagcaga tcatcctgcg gcagatcaac cacaactcca
     1441 tcccaattct gaatagtctg ctcatagtcc cgaattcgca gactcaaggg atgatgccgc
     1501 aaacggtctt cagcaatccc ctgtgtttga agcggagaaa tgtgcaagtc ctcatccacc
     1561 aggaacaaga ctccaatcgg gagagcaaga acattcaacg cctccgtcaa aacacggtgc
     1621 agccgactat aattgccgac tgcatatggc tgctccatga aaccctccga actcttcccg
     1681 atcctggcct cggacctcgc cgaaaccgac cgcgtgatcc gccagcgtct cggctcggag
     1741 gtggcgctga tcaacagcat cgccgactac atcatcggcg cgggcggcaa gcgcatgcgc
     1801 ccgctgctgc tgctgctctt cgcacgggcg ctgggttaca aaggagcgag caatcactat
     1861 ctactggcgg cagtggtcga gttcatccac accgccacgc tgctgcacga cgacgtggtc
     1921 gacgaatcgg cgttgcgccg cggccgccag accgccaatg ccgccttcgg caatgccgcc
     1981 tcggtgctgg tgggcgactt cctctactcg cgcg
//
LOCUS       DAOQUC010000793         2013 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_71674_length_2013_cov_3.311542, whole genome shotgun sequence.
ACCESSION   DAOQUC010000793 DAOQUC010000000
VERSION     DAOQUC010000793.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2013
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71674_length_2013_cov_3.311542"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(78..>2013)
                     /locus_tag="PKH07_12695"
     CDS             complement(78..>2013)
                     /locus_tag="PKH07_12695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35840.1"
                     /translation="ARHWPLTDDEAVYLKSIGCNTIKFPLYPSEVGIDEKRMMIWNTG
                     DRFEDLGVETWEPDWRSLDALLDWMIKHQMTPFVCPVAETREDWGTKAWMALHVPEEA
                     QRTVWYTKLVVDHVTAKYGDRVIYGWYENWWWNSYKHEKSAEFPEAFRAMLSKMYSGK
                     VRALNKSWMSNYRSFDDVEVPRLLVNNDIAEEAINSIRTYDLRRAMDLMHRDVLAGIR
                     DYISKVAPNAIWSGGCMLNELGGLNDIRSVRTPRTNATMRSCAVTSDIVGADLYAPQF
                     LYYSFYRTLAKISAVEGKRFSIVEAAATKPETFVWIADVGGPTAGTLAWSGKEDAYGF
                     IKFDGTRREENGRKFKELTEIILGNRERYRDYKPGHIQVYFPEETYYYTISGRNSMDA
                     YQHICDNMSPEELEPVLTDELTRLPKGSLIYVLERTLPLKAIEALNRLGKRVICPHEY
                     FIDEYGKHHDRKYVDEDFYARLLSTPDGAKLLDVFQRIEEKENNLAYRYYGTTIISPT
                     ELAPANMVVPGRENDLAQLIDGSIYEGVTFADKQQQEVVILHLPHPKTVYGAFVQFYE
                     GDGQDVKASALPDEISVLVSMDCVNYSEVAKVSGNDVTMRPHIRFEPVLARRIMLDFG
                     VNNRNSGLKIEEVGAIGQRR"
BASE COUNT          452 a          563 c          486 g          512 t
ORIGIN      
        1 tcattcatca gagcaagcgt ccgtaggagt gggcatagcc cacacgcctg caggcgctgg
       61 cctgctcaga agccccatca ccgtctctga ccgatagctc caacttcctc tatcttgagg
      121 ccactgtttc ggttgttcac accaaagtcc aacatgatcc gcctggcaag cacgggctcg
      181 aatcgaatat ggggtctcat ggtcacgtcg tttccggaaa cctttgccac ttccgagtag
      241 ttcacacagt ccatggagac caataccgag atctcgtccg gaagcgccga tgcttttacg
      301 tcctgtccat ctccttcgta gaactgcaca aaggccccgt acaccgtctt gggatgggga
      361 agatggagaa tcacgacctc ttgctgctgt ttgtcagcga aagtgacgcc ttcatagatg
      421 ctgccgtcaa tgagttgagc cagatcgttc tcgcgtcctg ggacgaccat attggcaggc
      481 gccaattccg tgggtgagat aattgtcgtt ccgtagtacc gataggctaa gttgttttcc
      541 ttctcctcaa tgcgctggaa gacgtccagc agtttggcgc cgtccggagt tgaaagaagt
      601 cttgcataga aatcttcatc cacatatttg cggtcatggt gcttgccgta ttcatctatg
      661 aaatactcgt gcggacagat cactcgtttc cctaacctat tgagtgcctc aatcgctttg
      721 agtgggagag tcctttcgag cacataaatg agcgatcctt tcggcaacct ggtgagttca
      781 tcggtaagca ccggttcaag ctcctcaggc gacatgttgt cacagatatg ctggtaggca
      841 tccatcgaat tgcggccaga aatcgtgtag tagtaggttt cctctggaaa gtagacctga
      901 atgtgacctg gtttgtagtc gcgataccgt tcccgattgc caaggataat ctcggtcagt
      961 tccttgaact tcctcccgtt ctcctcacga cgagttccat caaacttgat gaaaccataa
     1021 gcgtcttcct tgccagacca ggccaaggta cctgccgtcg gtccgccaac atcagcaatc
     1081 cacacaaagg tctcgggctt tgttgctgcc gcttcgacaa tggagaaccg cttaccctca
     1141 acggcgctaa tcttggcgag agtcctgtag aaagagtagt agaggaactg aggagcatag
     1201 agatcggctc cgacgatgtc acttgtgaca gcacatgagc gcatcgtggc atttgttcgc
     1261 ggtgtgcgta ccgatcgaat atcgttcagg ccgccgagtt cgttgagcat acaaccgcct
     1321 gaccagatcg cgttgggagc cacctttgaa atataatctc taattccggc caacacgtcc
     1381 ctgtgcatca agtccattgc ccgacgcaaa tcataggttc gaatactgtt gatggcctct
     1441 tcggcgatat cattgttcac cagcagcctc ggaacttcga cgtcgtcgaa ggagcgatag
     1501 ttgctcatcc atgacttgtt taaggctctc accttgcctg agtacatctt actgagcatg
     1561 gcacgaaatg cctctggaaa ctctgcactc ttttcatgtt tgtaggagtt ccaccaccag
     1621 ttctcatacc atccatagat gactctatcg ccgtacttgg cagtcacatg gtcaacgaca
     1681 agtttggtgt accagaccgt ccgttgcgcc tcttccggaa cgtgcaatgc catccaggct
     1741 ttcgtgcccc agtcttcgcg tgtttccgct acaggacaga cgaagggagt catctgatgc
     1801 ttaatcatcc agtccaatag ggcgtcgaga cttcgccagt caggctccca cgtctcaacg
     1861 cccaggtctt cgaatctatc gcccgtattc catatcatca tccgcttctc atcaatgcca
     1921 acctcgcttg ggtaaagagg aaacttaatc gtgttgcagc cgatactctt gagatagacc
     1981 gcctcgtcgt cagtcagtgg ccaatgcctg gcc
//
LOCUS       DAOQUC010000794         2012 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_71732_length_2012_cov_3.067961, whole genome shotgun sequence.
ACCESSION   DAOQUC010000794 DAOQUC010000000
VERSION     DAOQUC010000794.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2012
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71732_length_2012_cov_3.067961"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1578
                     /gene="feoB"
                     /locus_tag="PKH07_12700"
     CDS             <1..1578
                     /gene="feoB"
                     /locus_tag="PKH07_12700"
                     /inference="COORDINATES: protein motif:HMM:TIGR00437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HNT35841.1"
                     /translation="VAVGVALRGSGYSDVHLSYGQEIETEIASLIPLIESHWDLAATY
                     HPRWLALKLLEDDAIIRSAVCTDEIIAAAERSRNHLRVVFRDAPEIVIAERRYGFISG
                     ACQESVRSTVEIRHTLSDQIDAFVTNRLLGIPILLLLMWIVFQLTFTVGEIPTKFLEF
                     LVGLSRAFLNDVLPAGMLRSLVTDGILGGLGGIIAFIPMIMLLFLALAVLEDSGYMAR
                     AAFVMDRVMHRIGLHGKSFIPMLLGFGCSVPAILATRTLETRRDRFVTMMIIPLMSCG
                     ARLPIYVLFCGAFFSDSARGYVVLALYILGILLAIAMAKVFRGFVFRGATTPLVMELP
                     PYRIPTPEGVLIHTWERTWAFTKRVFTFLLVVSLIMWYLSNFPVGPDASLSPSERINF
                     SYAGRVGRLLAVPLKPVGLGDWRVGTALFSGFAAKEVVVTTLGTLYNVADTSEESISL
                     RQAIQDDPFFNPLRAFVLMVFTLIYLPCAGTFVAISRETKSYRWPVFCALYLTVLAWS
                     ASFLIYHLGMFLGIGIQ"
     assembly_gap    1736..1745
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1746..>2012
                     /locus_tag="PKH07_12705"
     CDS             <1746..>2012
                     /locus_tag="PKH07_12705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35842.1"
                     /translation="SPIGKDFFEIQRQQKRKTLLLFPLLVALYVVASLLVVLVVLVSF
                     GLFIFAYGRPWASPPDLARIILLCVITSGLFAVFHYTTALRSGAS"
BASE COUNT          347 a          535 c          538 g          582 t
ORIGIN      
        1 gttgccgtag gggttgccct tcgggggagt ggttattccg acgtacatct ttcgtatggc
       61 caggagatcg aaacagaaat tgcctcgctg attcctctca ttgaatctca ctgggacttg
      121 gccgcgacct atcatccgcg atggcttgcc ctgaagttgt tggaagatga cgcgatcatc
      181 cgctctgccg tctgtacgga tgagatcatc gcagcggcag agagaagtcg gaatcatctc
      241 cgcgtggtct ttcgcgacgc accggagatc gtcattgccg aacgccgcta cggcttcatc
      301 tcgggagcct gtcaggagtc agttcggagt acggttgaga ttcgacacac tctgtccgat
      361 cagatagacg cctttgtcac caaccgactg cttggaattc cgatccttct cctgctgatg
      421 tggatcgtgt ttcagttgac ctttaccgtt ggggagatcc cgacgaagtt tctggagttt
      481 ctggttggac tctctcgagc ctttctgaac gacgttctgc ctgcgggaat gctgcgatct
      541 cttgtgacag atggcatttt gggtggactt gggggaatca tcgctttcat cccaatgatc
      601 atgctgctgt ttctggcctt ggcggtcttg gaagacagcg gatacatggc acgcgcggct
      661 tttgtcatgg atcgcgtcat gcaccgaatc ggactacacg ggaaatcttt catcccgatg
      721 ctcctggggt ttggatgcag tgttcctgcc attctcgcca cccgaacact tgaaacgcgc
      781 cgagaccgat ttgtcaccat gatgatcatc cctctgatga gttgtggggc gcgactgccc
      841 atttacgtgc ttttctgtgg cgctttcttc agtgactctg ctcgtgggta tgttgttctt
      901 gcgctctaca tcctgggcat ccttctggcg attgccatgg cgaaggtctt ccgcggattt
      961 gtctttcgag gggcgactac acccttggtc atggaactgc caccttaccg gattcccacg
     1021 ccggaaggag ttcttatcca tacgtgggag cgtacgtggg cattcacaaa gagagtgttt
     1081 acgtttctgt tggttgtctc tttgatcatg tggtatctca gcaactttcc agtggggcct
     1141 gacgccagtt tgtcgccatc cgagcgaatc aacttcagct acgccggacg cgtcggacgt
     1201 ctccttgcgg tgccactgaa gccggtgggt cttggggact ggcgcgtggg gactgctctg
     1261 ttctcgggtt ttgcagccaa agaggttgtg gtgacaactt tgggcactct ctacaatgtc
     1321 gccgacacct ctgaagagtc aattagtctg cggcaggcca ttcaggacga tccttttttc
     1381 aacccgcttc gcgcctttgt tctcatggtt ttcactctga tctatctccc ctgcgccggt
     1441 acctttgttg ccatctcacg ggaaaccaag tcttaccggt ggccggtctt ttgcgcgctc
     1501 tatctcactg tgctcgcttg gagtgcctct ttccttatct atcatctggg aatgttcctg
     1561 ggtatcggga tccaatgaca ggttcaaccg tgttcatcgc aaggattgcc cgtgctgcgt
     1621 ctcgttttgt tttgtgcttg acgcaggagc cgtttgctct caaattctgg aacattacgt
     1681 ctggagagga tgaaccagca ttgtcttcaa gcggagtttg gtgatgaacc gactgnnnnn
     1741 nnnnngagtc caattggcaa agacttcttt gaaattcagc gacaacagaa acggaagaca
     1801 ctacttctgt ttcccttgct cgttgcgctc tacgttgtgg cgtccctgtt ggtcgttctc
     1861 gttgtccttg tctcctttgg attgttcatc ttcgcctatg gcaggccttg ggcaagtccg
     1921 ccagatttag ctcgcatcat tctcttgtgt gtcatcactt ccgggctgtt tgccgtattt
     1981 cactacacaa cggcgctccg ctccggtgcc tc
//
LOCUS       DAOQUC010000795         2011 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_71792_length_2011_cov_3.516360, whole genome shotgun sequence.
ACCESSION   DAOQUC010000795 DAOQUC010000000
VERSION     DAOQUC010000795.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71792_length_2011_cov_3.516360"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            69..1508
                     /locus_tag="PKH07_12710"
     CDS             69..1508
                     /locus_tag="PKH07_12710"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HNT35843.1"
                     /translation="MNRMSMLIVVCIGLVLASSSHAEVVINEFAYDDSGTDTHEYVEL
                     YNNGSTAVDIGGWLLINGVTVTSTTTTIVPGAFIPPGGFYVMGQIAGATQPLGGALQD
                     NKQYYDLFNALSELQDSVVVELNQTTGDVSTHGEPIGLGIQGRNILVEVGNVSGTGIS
                     DGSPNASFSIARYYDGVDTNNNEMDFGVLPATPNALNYSKGVVSVTSIQAGTPYVENF
                     DASDNTFVMEMLGSLSTVQCQNPAVADPLPTNLLVGACINPSVIPASPQGGNVGILFN
                     PRLTGSTGSGESGILNLTEPITDCEVEFYTYMPAKTQIANQYGVGTLVGMRGHPCPVY
                     GFTSPDKNGDTGIRVRWENDYTKGVHIWVEERLNNIVTPIGMPVTITSAGWIRVLLQV
                     NGTGVYALVGGAYGSSDGTVVQGTTTITQAGQVYFGYGQSTNQNTFTDCRPNTYDAFR
                     LSIPTMITPTPRPTSTPSSGVHYDLNSYR"
     gene            complement(1624..>2011)
                     /locus_tag="PKH07_12715"
     CDS             complement(1624..>2011)
                     /locus_tag="PKH07_12715"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNT35844.1"
                     /translation="WRPILKWFVKSLRDPEVARIGKRLREEKRDIVFYDAPCVVFFLA
                     KAYESYGEADCLLAAQNLMVAAQAMGIGSVMLGTVLMANRLSQARAFLRIPSDYQIRC
                     VVALGYSDEPVRDLPERKDNMLGWVE"
BASE COUNT          515 a          518 c          515 g          463 t
ORIGIN      
        1 gacaccatca gacctgtgtg atttcatgat ctgcgttttc acagagaaaa gaacaaggag
       61 agtcttcaat gaaccggatg agcatgttga ttgtggtttg tatcggcctg gttcttgcgt
      121 caagttccca tgcggaagtc gtcatcaatg aatttgcgta cgacgacagc ggaacggaca
      181 cccacgaata tgtggagcta tacaacaatg gctcgacagc agtggatatt ggtggctggc
      241 ttctgatcaa tggagtcact gtcacctcaa caacgacaac gatcgttccg ggtgcattca
      301 ttccgccagg tgggttctat gtcatgggac agatcgccgg agcaacacaa ccgctgggtg
      361 gcgcgttgca ggacaacaaa cagtactatg atttgttcaa cgctctgagt gaactccagg
      421 actcagtcgt tgtggaactc aaccagacaa cgggagatgt ctcaacccat ggagagccca
      481 ttggtctcgg aatccaaggg cgcaacatcc tcgtggaagt cggcaatgtc agcggcactg
      541 gcatcagtga cggaagtccg aatgcctctt tcagcatagc tcgctactac gacggtgtgg
      601 acaccaacaa caacgaaatg gatttcggtg ttttgcctgc caccccgaat gctctcaact
      661 acagcaaggg cgtcgtcagt gtcacctcga tccaggcggg aacaccctat gttgagaatt
      721 ttgacgcatc ggacaacact tttgtgatgg agatgctcgg tagtctttcc actgtccagt
      781 gtcaaaatcc tgcggtcgct gacccgctgc ccaccaacct gcttgttggt gcttgcatca
      841 atccctctgt gattccggca tcccctcaag gaggcaatgt cggaatcctt ttcaacccaa
      901 gactgacagg ttcgactggt tctggagaga gtggaattct caatctcaca gaaccgatca
      961 ccgactgcga agtagagttc tacacctaca tgcctgccaa gactcagatc gcgaaccagt
     1021 atggcgtcgg cactttggtt gggatgcgtg gacatccctg tccggtctac ggcttcacca
     1081 gtccagataa gaatggagat actggcattc gtgttcgatg ggaaaatgac tatacaaagg
     1141 gtgttcatat ctgggttgaa gaaagactaa acaacattgt cactccaatc gggatgccag
     1201 tgacgatcac gtctgcgggt tggattcgag ttctgctgca agtcaatgga actggtgtct
     1261 acgctcttgt tggtggtgcc tacggatcca gcgacggaac tgtggttcag ggaacaacaa
     1321 cgatcacaca ggcagggcag gtttattttg gctatgggca gagcacgaac caaaacacgt
     1381 ttacagactg ccgacccaat acatatgatg cgttccgtct gagcattccg acgatgataa
     1441 cgccaactcc aaggccgacc agtacaccga gcagtggagt ccattacgat ttgaactcct
     1501 atcggtaagg gatatctgga aagatttgca gaatcaatga ctcgggcaca gaatgctgcc
     1561 tgagttgttg ttttgtcctt tgaaaatcag atcctgttgt cttgaatcaa tgcctggcgg
     1621 acactactcc acccaaccga gcatattgtc cttacgttct ggcagatctc gaacaggttc
     1681 atctgaatag ccgagggcaa caacacaacg aatctgataa tcggaaggga tgcgtagaaa
     1741 cgcccgcgct tgtgagagcc ggtttgccat gagtaccgtg cccaacatga cactgccgat
     1801 acccattgcc tgtgcggcaa ccatgaggtt ctgcgccgcc aagagacagt cggcttcccc
     1861 ataggactca tacgccttcg ccaggaagaa caccacacaa ggagcatcat agaagacaat
     1921 gtcacgtttc tcttcccgaa ggcgcttgcc aatgcgggca acttcgggat cgcgcaacga
     1981 tttgacaaac catttcagaa tcggtcgcca a
//
LOCUS       DAOQUC010000796         2011 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_71814_length_2011_cov_2.744376, whole genome shotgun sequence.
ACCESSION   DAOQUC010000796 DAOQUC010000000
VERSION     DAOQUC010000796.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71814_length_2011_cov_2.744376"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1201)
                     /locus_tag="PKH07_12720"
     CDS             complement(<1..1201)
                     /locus_tag="PKH07_12720"
                     /inference="COORDINATES: protein motif:HMM:NF024810.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HNT35845.1"
                     /translation="MKQAIGLLLVVGVLFGLGNVVPASAEQWQALPSYEVGRRGHTAV
                     YDGRVDRMVVFAGQTSNGYVNSCLQMNLAAGSEAWSGLSPSGEVLPSPRAFHTAIVYP
                     TRNMDAMIVFGGMNQVGRVTNYFNDLWALSMIQGSEAWNQIDTKGTLPAVRESHSAIY
                     DPGSATMIVFGGRNGTTYFDDLWVFDFSSSSWALHANSGKGPTSRAGHSAVYDAAKSR
                     MLVYGGYTSSGAYLDELWALDLTAVDGEWKKLSPSGKGYHAVAWHSAVFDDNGTRMLL
                     FGGTDGMPRNYLQCLSFTTDENGEWVALNTVGTVPVARNAFPAVLDPKDERMVLFGGV
                     GSLTPYLGSTHELNWRIIATATPTRTPTRTATDTAYPTITDTATETEAPATLTPTPTQ
                     PPYWYNEV"
     gene            complement(1568..>2011)
                     /locus_tag="PKH07_12725"
     CDS             complement(1568..>2011)
                     /locus_tag="PKH07_12725"
                     /inference="COORDINATES: protein motif:HMM:NF012622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I domain-containing protein"
                     /protein_id="HNT35846.1"
                     /translation="PSDGASLNSYELVQFQGAGQDFEDGILSGDSLVWSSDVDGVLGS
                     GVSVESNALSPGTHHITLQVFDSALKSATQMITVTVDESLVLVADIHRDGKINDLDMI
                     KLASYWQTTPEAAKWDAKVDLNQDQAVDAQDALLLIEAIVAESGY"
BASE COUNT          507 a          539 c          514 g          451 t
ORIGIN      
        1 tgacttcgtt ataccagtag ggaggctgag tcggagttgg cgtgagtgtt gccggtgctt
       61 ctgtttcagt tgccgtgtct gttatcgtgg gataagccgt gtccgttgca gttctggtcg
      121 gtgtccgcgt cggtgttgcc gtagcgatga ttctccagtt gagctcgtgt gtcgaaccga
      181 ggtatggggt cagtgagccg acaccgccga aaagcaccat tctttcatcc ttcggatcaa
      241 ggactgcagg aaaggcattt cgtgccacag ggactgttcc gacagtgttc agagcaaccc
      301 attctccatt ctcatccgtg gtgaaagaca gacattggag atagtttctt ggcataccgt
      361 cggttccacc gaaaagaagc attcgagtgc cgttgtcgtc aaacacggca ctgtgccaag
      421 cgaccgcatg atatcccttg ccagaaggac tcagtttctt ccactcacca tccactgcgg
      481 tgaggtcaag agcccagagt tcatccaaat aggctccgga cgaagtgtat ccaccgtaga
      541 caagcattcg gctcttggca gcgtcataga cggcgctgtg acccgcgcga gaggtcggtc
      601 ccttgcccga gttggcatgc aatgcccaag aagatgagct gaaatcaaaa acccagagat
      661 cgtcgaaata ggttgtccca tttctgccac caaagacgat catcgtggca gaacccggat
      721 cgtagatggc agaatggctc tcgcggacag ccgggagagt cccctttgtg tcaatctgat
      781 tccaagcctc tgaaccctga atcattgaaa gtgcccaaag gtcgttgaag taattggtga
      841 ccctaccgac ctggttcatc ccgccaaaaa cgatcatggc atccatattg cgtgtgggat
      901 agacgatggc ggtatggaag gcacggggag aagggagcac ttcacctgat ggcgaaagcc
      961 cggaccacgc ttccgaacca gcagcaaggt tcatctgcag acagctgttg acatagccgt
     1021 ttgaggtctg gcctgcgaag acgaccatgc ggtcaactct gccatcatag accgccgtat
     1081 gtcctctcct gccgacttcg taacttggca gcgcttgcca ctgctccgcc gaagcgggaa
     1141 cgacattgcc caagccgaac aaaacgccaa cgacgagcaa caaaccgatt gcctgcttca
     1201 tttccagact ccttctgacg gttcgaccgt caagtaacca gaagccaaac accgacgcca
     1261 atttcaaagg aaaactgaac caacaacctt gataaatgta tcgagggaga gacactcatg
     1321 gtttctgccc ctgccgaaca cctgaaccct attccgaccc gtcactgata ccacaatcag
     1381 ataggggtca aaagaaaacg aaaagcaaaa taacctccca gcaaacttgc tattaagata
     1441 gcctgttttg gcgtagaatg tcaacaaatt tagcagaaat ggatataatc tacggaagcc
     1501 tcccaacgtc tgcagggagg cttccgtgaa gagttcagca ctccgagaag tctttccttt
     1561 cggagtctca ataaccgctc tcagccacta tagcttctat gagaagaagc gcgtcttggg
     1621 catcaacagc ctggtcttgg ttaagatcca ccttggcatc ccacttggct gcttcgggtg
     1681 ttgtctgcca gtaggaggcc agtttgatca tgtccagatc gttgatcttc ccatctcgat
     1741 ggatgtctgc aacaagtacg agagattcat ccaccgtgac ggtgatcatc tgggtagcgg
     1801 acttcaatgc gctgtcgaaa acttgaagcg tgatgtgatg cgttcctggt gaaagtgcat
     1861 tggactcaac tgagacccca gagcccagaa cgccgtcaac gtccgatgac catacgagtg
     1921 agtcgccact caagatgcca tcttcaaagt cttgaccagc gccttgaaac tgaaccaatt
     1981 catagctgtt cagagatgca ccgtcagagg g
//
LOCUS       DAOQUC010000797         2009 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_71945_length_2009_cov_2.744626, whole genome shotgun sequence.
ACCESSION   DAOQUC010000797 DAOQUC010000000
VERSION     DAOQUC010000797.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71945_length_2009_cov_2.744626"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1198)
                     /gene="leuC"
                     /locus_tag="PKH07_12730"
     CDS             complement(<1..1198)
                     /gene="leuC"
                     /locus_tag="PKH07_12730"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008410940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HNT35847.1"
                     /translation="MGMTLAEKILAAHCGQQQVHPGQFILADVDIALGNDITAPLAIE
                     EFQGCGAEKVFDKDRVVLIPDHFAPNKDIKAAQQCKMMRQFARAQNLTNYFEVGEMGI
                     EHALLPEQGLVGPGDVVIGADSHTCTYGALGAFATGVGSTDLAFTLATGKAWFRVPES
                     MKFVFKGTLNPWVTGKDLILYTIGDIGVEGARYRAMEFTGEAISNLSQDSRFAMSNMA
                     IEAGGKAGLIAPDSTTREYIKNRFKREPRFHQSDPDAQYCDVREYDVARIQPQVSLPH
                     LPSNAKAVGDVGDIAIDQVVIGSCTNGRMEDLRIAAKIMAGRKVAKGIRCIILPGTQQ
                     IYLEALREGLVEQMVQAGAAFSTPTCGPCLGGHMGILAEGERALATTNRNFVGRMGHP
                     KSEVYLC"
     gene            complement(1292..>2009)
                     /locus_tag="PKH07_12735"
     CDS             complement(1292..>2009)
                     /locus_tag="PKH07_12735"
                     /inference="COORDINATES: protein motif:HMM:NF023178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3307 domain-containing protein"
                     /protein_id="HNT35848.1"
                     /translation="PFNIGLVSSLVLAHLLADFPLQADVVFRWKKKGSFGVAIHAAIF
                     VLVAYLLLLPMVRTVSVVVMIGSLGFVHFWIDETKLRLFRRIEVDNLWAFLADQALHF
                     MSLLLAAWLFAKHAANVRLEPEMFRFTLQPMTCGVVTGLIVSSYFGVLITHYMEKMLF
                     GSAYHNENLRPADKYLGILGRLCVTSAFLPESWFVLSLTVALGFLFFLSWRVKGWTSR
                     LFVYYLSSAVLASLVGMFLM"
BASE COUNT          496 a          600 c          502 g          411 t
ORIGIN      
        1 cgcagagata cacttcactc ttgggatgtc ccattcttcc gacgaaattg cgattcgttg
       61 tcgccaaagc ccgctcgccc tccgcgagaa ttcccatgtg gcctcccaga cagggaccgc
      121 atgtgggtgt ggagaacgcg gctcccgcct gcaccatctg ctcgaccaga ccttccctga
      181 gcgcttcaag ataaatctgc tgcgttccgg gcaggataat gcagcgaatg cccttggcga
      241 ctttgcgtcc tgccatgatc ttcgcggcga tccgcaaatc ctccatgcgt ccgttggtgc
      301 aggaaccgat caccacctga tcgatcgcga tgtcacccac atcaccaacc gccttggcat
      361 tggatggaag atggggcaga gagacctgag gctgaatcct ggccacgtcg tactcccgaa
      421 catcgcagta ttgggcgtca ggatcgctct gatggaacct gggttcacgc ttgaagcgat
      481 tcttgatgta ctcccgagtg gtgctgtcgg gtgcaataag tccggctttg ccaccagcct
      541 cgatagccat gttggacatg gcaaaacggc tgtcttgtga cagattcgat atggcctctc
      601 cggtgaactc catcgcgcgg tatcgagctc cttccacgcc gatgtctcca atggtgtaaa
      661 ggatcaaatc cttccccgtc acccagggat tgagggtgcc tttgaagacg aacttcatgc
      721 tctcgggaac tcggaaccag gcctttcctg tcgccagagt aaacgccaga tccgttgaac
      781 ccactcctgt cgcgaaagcc cccagagcgc cataggtaca cgtgtgcgag tccgccccaa
      841 tcacgacatc tccagggccg accaggccct gttcgggcag aagggcgtgc tcaataccca
      901 tctctccaac ctcaaagtag ttggtcagat tctgcgctcg tgcaaactgc ctcatcattt
      961 tgcactgctg ggcagccttg atatctttgt ttggtgcgaa gtggtctgga atcagaacca
     1021 cccgatcttt gtcaaagact ttctccgctc cacagccctg gaactcctcg atggcaaggg
     1081 gcgcagtgat atcattgccc aaagcgatgt ccacatctgc aaggatgaac tgccctggat
     1141 gtacttgctg ctgtccacaa tgcgccgcca aaatcttctc cgccaacgtc atgcccatct
     1201 tgtgcttcac tccattttct gacccgatta gaaccaacgc tgcaggaagc gttgtagctc
     1261 gacggatcga tacttgttca atacgaaccg attacatgag aaacatgccg accaggctcg
     1321 ccagtacggc actggataga taatacacga aaagccgact tgtccagcct ttcacgcgcc
     1381 aggacagaaa aaaaagaaat cccaacgcca cggtcagaga aagaacgaac cacgactcag
     1441 gcaaaaaggc actggtgacg cacagccttc cgagaatccc cagatacttg tccgccggtc
     1501 tgagattttc gttatggtag gcgctgccga acaacatctt ctccatataa tgcgtgatca
     1561 gtacgccaaa atacgacgac acgatcaaac cggtgaccac accacacgtc atgggttgaa
     1621 gagtgaaccg gaacatctct ggttcgagac ggacgttggc cgcgtgcttt gcaaacagcc
     1681 aggccgccag aagcagagac atgaagtgca gcgcctgatc cgccagaaat gcccacaaat
     1741 tgtccacctc aatgcggcga aacagacgca gtttggtctc atcgatccag aaatggacaa
     1801 agccgagtga cccgatcatg accacgacgg aaacggtgcg taccatcgga agaagcagca
     1861 ggtaggccac aaggacaaat atcgccgcat ggatcgcaac accaaaactc cccttctttt
     1921 tccatcggaa gaccacgtcc gcctgaaggg gaaagtcggc cagaaggtgc gccaacacca
     1981 gacttgaaac cagaccgata ttgaacggc
//
LOCUS       DAOQUC010000798         2008 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_71999_length_2008_cov_2.973374, whole genome shotgun sequence.
ACCESSION   DAOQUC010000798 DAOQUC010000000
VERSION     DAOQUC010000798.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2008
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71999_length_2008_cov_2.973374"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..551
                     /locus_tag="PKH07_12740"
     CDS             <1..551
                     /locus_tag="PKH07_12740"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNT35849.1"
                     /translation="IEMNANMMQRIIGDFLDFHAMEDGQLTLNLQPANLNVVASEVVE
                     ANRDYAESKGIQIEFVSGGDLPFVSADPARIQQVIQNLVGNAIKFCGEGSQVWVKTRA
                     SQDRVFVEIQDTGPGLTEEDVQQAFQKYTKLSNKPTGGESSSGLGLAISKQLIDMHKG
                     EIGVCTGPDGGALFWFSLLASL"
     gene            618..1970
                     /locus_tag="PKH07_12745"
     CDS             618..1970
                     /locus_tag="PKH07_12745"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNT35850.1"
                     /translation="MSMEERLQAEVTRRDFLKATSAVGLAAGLGLATRDVRGKEPEPT
                     TSSSDKIRVGFIGVGPRGSHLMNEFLEQSDVLVTAVCDVYEPRIEEAIKRAGVQAKGY
                     RDFRKLLDESDCDVIVVATPPHWHALISICAMQAGKDVYCEKPMCLYPDEAVAVASAA
                     KKYQRVTQLGTQIHAMNNYRRVVEIVRSGVLGKIMNVRTAVTLNESPGRFKGVEDSDP
                     PKGLDWDLWLGPGPNKPFNKTLFEDGGHRYFKEFVMSWVNELGPHIMDLAFWAMNPGA
                     PSAALAAGGRFVMEDVTDIPDTVDILYEYPGFSMNFIHTAGNGYNFGFGASPDRGRRL
                     GVFFHGTNGTLAADYSNYSIFLEGIKQEDFSPPEETIPASPGHVREFLDGVKTRVQPL
                     CNFEYHFPMTLAIALGHVALFSGERVEWDAKRQRVVGSRKARQMAQSHYRKPWSLPRV
                     "
BASE COUNT          453 a          471 c          611 g          473 t
ORIGIN      
        1 agattgagat gaacgccaat atgatgcagc ggatcattgg cgacttcctt gatttccatg
       61 ccatggaaga tggtcagctg actttgaacc tgcaaccagc gaatttgaac gtggttgctt
      121 cggaagttgt tgaagcgaat cgggactatg cggaatcgaa ggggatccag attgagtttg
      181 tttccggggg cgacttgcct ttcgtctctg ccgatccagc tcgaatccaa caggtgattc
      241 agaatctggt tggcaatgcc atcaagttct gtggagaagg ttctcaagtg tgggtgaaaa
      301 ccagagcatc tcaggacagg gtctttgtcg agattcagga tacaggcccg gggctgacag
      361 aggaagacgt gcagcaagca tttcaaaagt acaccaaatt gagcaacaaa ccgactggcg
      421 gggagtccag ttcgggtctt ggactggcca tcagcaaaca gctcattgat atgcacaaag
      481 gggagatcgg agtctgcact ggccccgatg ggggggcgct tttctggttc agcctcttgg
      541 catcgctctg agtcgttctg tcagtgccgg cgcgaggaca cgttccacaa ctcaggacct
      601 gttgaaagga gttcaacatg agcatggagg agagacttca agcagaagtc acaagaaggg
      661 attttctcaa ggcaacgtcg gcggttggat tggctgccgg acttggactg gcaaccaggg
      721 atgtccgagg gaaagagcca gagcccacca cctcgtccag cgacaagatt cgggttggat
      781 ttatcggtgt tgggccacgt ggttcgcact tgatgaacga attccttgaa cagtcggacg
      841 tcctagtcac cgctgtctgt gatgtctatg agcctcgtat cgaagaggcg atcaagcgcg
      901 ctggtgtaca agcgaagggt tacagggatt tcagaaaact tctggacgaa tccgactgcg
      961 atgtcattgt ggttgccacg ccgcctcatt ggcacgcctt gatttccatt tgtgccatgc
     1021 aggcgggcaa agatgtctac tgcgagaagc caatgtgtct ctaccctgac gaggcggttg
     1081 cggttgcgtc ggctgcgaag aagtaccaac gagtcacgca gctgggaacg cagattcatg
     1141 ccatgaacaa ctatcgtcga gtggttgaga tcgttcgttc cggagtgctc ggcaagatca
     1201 tgaatgttcg tactgcggtc actttgaacg agagtcccgg gcgtttcaaa ggcgttgagg
     1261 acagcgaccc acccaagggg ctcgattggg atctctggtt ggggcctggt ccgaacaaac
     1321 ctttcaacaa gactcttttt gaggatggcg ggcaccggta tttcaaggag ttcgtcatga
     1381 gttgggtcaa tgaactggga ccgcacatca tggatctggc cttttgggcg atgaatcctg
     1441 gtgcacccag cgctgctctt gctgctggtg ggcgttttgt catggaggat gtcacggaca
     1501 ttccggatac cgtggacatt ctctatgagt acccgggatt ttcgatgaat ttcattcata
     1561 ccgccggcaa tggatacaat tttggcttcg gtgcttcgcc cgacagggga cgcaggctgg
     1621 gcgtcttctt ccatggaacc aacggaacgc ttgctgcgga ctattcgaac tactcgattt
     1681 tcctggaagg gatcaagcag gaggatttct caccgcctga ggaaacaatt cctgcctcgc
     1741 caggtcatgt gcgggaattc ctcgatgggg tcaagacacg cgtgcagccg ttgtgtaact
     1801 ttgagtatca tttccccatg actcttgcga tagcgcttgg gcatgtggca ctgttttcgg
     1861 gggagcgtgt tgaatgggat gcgaagcgtc agcgggttgt cgggagcagg aaggcgcgtc
     1921 agatggcgca gtcgcattac cgaaaaccgt ggtctttgcc gagagtgtag gaaggtgccc
     1981 gcatgtcaca acgcatggac agaagagt
//
LOCUS       DAOQUC010000799         2008 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_72001_length_2008_cov_2.820276, whole genome shotgun sequence.
ACCESSION   DAOQUC010000799 DAOQUC010000000
VERSION     DAOQUC010000799.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2008
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72001_length_2008_cov_2.820276"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..94)
                     /locus_tag="PKH07_12750"
     CDS             complement(<1..94)
                     /locus_tag="PKH07_12750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009328949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HNT35851.1"
                     /translation="MADCIFCKIVKGEIPCKKSEETEHFLAFHDI"
     gene            317..1105
                     /locus_tag="PKH07_12755"
     CDS             317..1105
                     /locus_tag="PKH07_12755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35852.1"
                     /translation="MAEFPSSVTIQSVIANLSDFTAGLLHDLTTPYAALTGYLSLLLD
                     RVKSLPGHDDSEEIATFIKGLRESQERFEGFVSRLRWLAEPLNLSLEPVPLCEVFRSV
                     IEEESLSETIAKLRRGLSFKTRVRAGNDYALLDTPYFSMAMRTLLLAVVTSAEGRGSI
                     RCTLDRTLSPEAPLLWEAIASEGAPTKKGFFRFTIRDGSGAWAGVELTQLFLPLQSNT
                     PKRLHEVNRVEMAIAYQVIRAHGGFFRLTESRDGASLLMVYLPE"
     gene            1102..>2008
                     /locus_tag="PKH07_12760"
     CDS             1102..>2008
                     /locus_tag="PKH07_12760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35853.1"
                     /translation="MKIRANKPRSSSPSLGRDSRKVPFALCQTLPMISRLSILSLFLL
                     IPLFVTTLGTDRYLLPKTALLLWTSSFLAACLFCHAVICGSAAFQLRLRLPILLYLLL
                     ISMSLSRSITLGSSLDVLLQWFSFFIVYQSVCIFFRQRAARLSLLLVQLGAGLSSLAL
                     LVTGLGLLPVKAPMASTFGNRNFLSEYLVLSLPVGIAVLSTAKSRSEKIFLLLSVLTQ
                     FLALVMASSQGAWLGCFASGIFLLLLQRGRVKHILGSLLGNQGLDEAVSFAGKPRVGA
                     DSAHTTADLGLLEKRPLRTKIVPRSV"
BASE COUNT          411 a          531 c          509 g          557 t
ORIGIN      
        1 tgatgtcatg aaaggccaaa aaatgctctg tctcctccga cttcttgcag ggaatctcac
       61 ctttcactat cttgcagaag atgcagtcag ccatgactaa tcctcccatg ggaactcaaa
      121 atttccgtct gccttactga cagaaatgtt acagatgaag gcgacggcgt caagtgaagt
      181 tgttttcgtc cacagttgag cagttgacat cacaccaatg agttcggtac cctcagacaa
      241 tatggaaagt gatagttcct tgctaaaggc aaattctggt tcggagagga cacgatcaga
      301 tcttcacgga aacagggtgg cggaattccc atcctcggtg actatccagt ccgtgatcgc
      361 aaatctgtcc gatttcactg cgggcctgct gcatgacctc acgacgccct atgccgctct
      421 gacgggctat ctgagcctct tgcttgaccg agtcaagtca ttgccaggcc atgatgattc
      481 cgaggagata gcgaccttca tcaaagggct tcgggagagt caggagcgat ttgaagggtt
      541 tgtctcaagg cttcgttggc tggcagagcc tctgaacctt tcattggaac ctgttccttt
      601 gtgcgaggta ttccggtcag ttatcgaaga ggaaagcctt tccgaaacaa ttgcaaagct
      661 taggcgaggc ctgtccttca agacaagagt cagggcaggg aacgactacg ccttgcttga
      721 cacgccgtat ttctcgatgg caatgagaac actgcttctg gctgtcgtca cctctgctga
      781 aggcaggggt tccatacggt gcacgctcga cagaactttg tctcctgagg ctcctctgct
      841 gtgggaggcg attgcctctg agggagcacc aacgaagaag ggtttcttcc gtttcacgat
      901 tcgagacggc tccggtgcct gggcaggcgt tgaactgaca cagttgttcc tacccctgca
      961 atcaaataca cccaagcgtc tacatgaggt gaacagagtc gagatggcaa ttgcctatca
     1021 ggtcattcgt gcacacggcg gattcttcag gcttacggag agccgagatg gtgcttcctt
     1081 gttgatggtc tatcttcccg aatgaaaata agagccaaca agccccgttc ttccagtcct
     1141 tctctgggac gtgatagtcg caaggtaccc tttgctctgt gtcagactct gccgatgatc
     1201 tctcgactga gcattctgtc tctgttctta cttatccctc tatttgtgac aacgcttggg
     1261 actgatcggt atcttctgcc gaagactgcg cttctgcttt ggacttcatc atttcttgca
     1321 gcatgccttt tttgccatgc tgtgatctgt ggatccgctg ctttccagct gcggttgaga
     1381 ctcccgattc tcctctacct tcttctaatc tcaatgtctc tcagccgctc tattactctc
     1441 ggttccagtc tggatgtttt gcttcaatgg ttctctttct ttattgtcta tcagagtgtc
     1501 tgcatctttt ttcgacaacg cgcagcaaga ctctccctgc tactcgtgca gttgggtgcg
     1561 ggtctctcaa gtcttgcgct gcttgtgact gggcttggct tgttacccgt caaggcgccc
     1621 atggcttcca catttggcaa caggaacttc ctctccgagt atctggtact ctctttacct
     1681 gttgggatcg cggtactctc gaccgcaaaa tcgcgttctg agaagatctt tcttcttctg
     1741 agtgtcttaa cccagtttct tgcgctcgta atggcgagta gccagggagc ctggttgggc
     1801 tgttttgcgt ccggaatttt cttgctgttg cttcagcgtg gcagagtcaa acacatcctg
     1861 ggttcactcc tgggaaacca aggtctggat gaggcggttt cctttgcggg caagccccgc
     1921 gtaggagcgg actcagccca cacaaccgca gaccttggcc tgctcgaaaa gcggccgttg
     1981 cgcaccaaga ttgtgccgag gagtgttg
//
LOCUS       DAOQUC010000800         2004 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_72241_length_2004_cov_2.615700, whole genome shotgun sequence.
ACCESSION   DAOQUC010000800 DAOQUC010000000
VERSION     DAOQUC010000800.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72241_length_2004_cov_2.615700"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="PKH07_12765"
     CDS             <1..187
                     /locus_tag="PKH07_12765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35854.1"
                     /translation="LESEAIVFLRLDTDGPADPARTLKYYRDKGLLHGTRIGKKVRYA
                     KQELLRFIDDMTERPRK"
     gene            269..1474
                     /locus_tag="PKH07_12770"
     CDS             269..1474
                     /locus_tag="PKH07_12770"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNT35855.1"
                     /translation="MELRISITRDNRPQRAKPWLVRWYGDYDVRTGKQRRYCKSFTLR
                     KDAERFVEEKKAEFQAGLPADQRDITLKQLCRKFMDTRPKAYSKAYRRGLEETIDRLL
                     TYFSAVTSIRRIRIEDAEAFIASLEPTSPQYIAKGKELSDSALALHLRQAKTIFSTAQ
                     AWGYLKANPFQKVKLGNLRRREWHFISVEEFNAIIDQTPSLREKALYGVMYWCGLRFG
                     EAANLRWEGRCIDFERGEISVFNRPATKTLPPFRVKDYEARIVPMNTWIMGMLKQLHA
                     LRQDHCPYVFLTPERLERVQDRWTTLQKARKSHDWENRFMLNSVLRDFKTRCCRAGIK
                     TDLKLTLHGLRKSWATNLANSGKVPPKTLLELGGWSSIRTCEEFYLKNTDANRQRACQ
                     VLNELAGGE"
     gene            complement(1589..>2004)
                     /locus_tag="PKH07_12775"
     CDS             complement(1589..>2004)
                     /locus_tag="PKH07_12775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35856.1"
                     /translation="GHPDPSAGYTGANVYGINLEGDYDPAVGGPYYLTTGPLDCRHFR
                     TVQVAFMSWLNIDSSDYAQCQFEISTNGADWTVLWNNTPDPITDSEWQEMEFDISKYA
                     DDNPTVYLRWGYQIIDRAYPYSGWNIDDVQLWGTL"
BASE COUNT          493 a          555 c          535 g          421 t
ORIGIN      
        1 cctggaatct gaagccattg tatttcttcg tctcgacacc gatggaccgg cagatcctgc
       61 tcgaaccctc aaatactacc gcgacaaggg cctgctccat ggcacccgca tcggcaagaa
      121 ggtgcgatac gccaagcagg aattgctccg attcatcgac gacatgacgg aaagacccag
      181 aaaatgaacc acccccttcg cagcaggtgt ctccagcggt ataccaccct gacctgacac
      241 gctgcatcag gctcaggagg caggaactat ggaactgagg atatcgataa cacgggataa
      301 ccgaccacag agagccaaac cgtggctggt ccgatggtat ggcgactacg acgttcgcac
      361 cgggaagcaa cgccggtact gcaagtcctt caccctccgc aaggatgccg aacggttcgt
      421 cgaagagaag aaagccgaat ttcaggcagg tctgcccgca gaccaaaggg acatcaccct
      481 gaagcaactg tgccgcaagt tcatggacac ccgacccaaa gcctattcca aggcctaccg
      541 acgaggcttg gaggagacca tcgaccgcct cctgacctat ttctctgcgg tgacctcaat
      601 ccgcagaatc cgcatcgaag atgctgaggc gtttattgcc agcttggaac caaccagtcc
      661 ccagtatatt gcgaagggca aggaactgtc cgactcagcc cttgccctcc atcttcgtca
      721 agccaagacg atcttctcca cggctcaggc atggggctat ctcaaagcca accccttcca
      781 aaaagtcaag ctgggcaatc ttcgaagaag ggaatggcac ttcatctctg tggaggagtt
      841 caatgccatc atagaccaga cccccagcct ccgggaaaag gccctttacg gcgtgatgta
      901 ctggtgcggt ctgaggttcg gggaggctgc caatctccgt tgggagggtc gatgcatcga
      961 tttcgagagg ggggagatct cggtcttcaa tcgacccgcc accaagacgc ttcctccctt
     1021 cagggtcaag gactacgagg cacgcatcgt tcccatgaac acctggatca tgggcatgtt
     1081 gaagcaactc catgctctca ggcaagatca ttgcccctac gtttttctga cgcctgaaag
     1141 gctcgaacgg gtgcaggatc gatggacgac tctccagaag gcacggaaga gccatgattg
     1201 ggaaaacagg ttcatgctca acagtgttct tcgggatttc aagacaagat gttgtcgggc
     1261 aggaataaag acggatctca agctcacgct ccatgggctg aggaaaagtt gggcgaccaa
     1321 tctggcgaac agtggcaagg ttccacccaa gaccctgctg gaactgggcg ggtggtcgag
     1381 catccgaacc tgtgaagagt tctatctcaa gaacactgat gccaacaggc aaagggcgtg
     1441 tcaggtgctc aatgagttgg cagggggaga atgaaggctg cagtccacca tagaccacag
     1501 ccttgatagg tttttcctac gcacaggggt tcatgctcag gttcttctgt ttttgctgct
     1561 gatttccttc tgaccatgaa cgattgtgct acagtgttcc ccacaattga acatcatcaa
     1621 tgttccaacc ggagtaggga taggcccggt caatgatctg gtacccccac cgtaaataga
     1681 ccgtggggtt atcatcggca tatttactga tatcgaactc catctcttgc cattcactgt
     1741 ccgtgatagg atctggggtg ttgttccaca gaaccgtcca atcagcccca ttggtagata
     1801 tttcgaattg gcactgagcg tagtctgagg agtcgatgtt caaccaactc atgaaggcaa
     1861 cctggaccgt tcggaagtgc ctgcagtcaa ggggtcccgt ggtcaggtag tagggacctc
     1921 ctacggcagg gtcgtagtcc ccttcaaggt tgatcccata gacgtttgcc ccggtatagc
     1981 ctgcactggg gtcaggatgc ccat
//
LOCUS       DAOQUC010000801         2001 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_72382_length_2001_cov_2.811408, whole genome shotgun sequence.
ACCESSION   DAOQUC010000801 DAOQUC010000000
VERSION     DAOQUC010000801.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72382_length_2001_cov_2.811408"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..574
                     /locus_tag="PKH07_12780"
     CDS             <1..574
                     /locus_tag="PKH07_12780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35857.1"
                     /translation="LPGEGVVETDWLVGKPLGGKYFVQRITLDLRGRTRQDVVREWVT
                     TLVSAIRRHDSRHLITVGEIPWAAVFPGAKSVFHPSEEEYGLDFVSVHFYPESGKTDQ
                     ALAALFSYDIGKPLVVEEMFPLKCSIEDLQSFVDGSRDRVSGYFGFYWGRTIDEYSQP
                     PVTIQAAILKAWLEYFRDHGSTVLHPTEAK"
     gene            complement(558..>2001)
                     /locus_tag="PKH07_12785"
     CDS             complement(558..>2001)
                     /locus_tag="PKH07_12785"
                     /inference="COORDINATES: protein motif:HMM:NF015557.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HNT35858.1"
                     /translation="TTWCKNNKVSPSRLLLPLSYLAVLGGTCTLIGTSTNLVVQGLME
                     EAVHVAEPGLRLALRPMGLFELSFVGVPCTLVGVLYLFFVGRHLIPTRKGFLEQIRGC
                     SRDYLINVRVESGYRFISKTVEEAGLRHLHGLFLIEIDRKGEIISPVGPEQTLFEGDM
                     LTFTGIISTIVDLEKIPGLVPIADDSYETRMDVRREKLLCESVVSRTSPMVGQSIREG
                     NFRARYNAAVVAVHRGGERLKGRVGDIVLLPGDTLLLQAGPHFVAAHRNDPDFYLVSG
                     VEDSSVVRHDKAAIALALLAVLIVLMATGVTSIVMAAFIVAGLMLATGCISVSQARQS
                     LEWQTLLTICAAFGIAKGLENSGCVATVSQIITDIFSRGGPKAVLSGVFLMTMGFTSL
                     VGNNSAAALMFPFGVAIALNMGVSPRPFVMMITLAASACFMTPVGYQTNMMVYGPGGY
                     RFSDFLRVGAVLNCLLLLVSVLLVPIIWPL"
BASE COUNT          524 a          513 c          534 g          430 t
ORIGIN      
        1 acttccgggg gagggggttg tggagacgga ctggctcgtt ggcaagcctc ttggtggcaa
       61 gtactttgtt caacgcatca ctcttgatct ccgtggccga acgagacagg atgttgtacg
      121 ggagtgggtt accacacttg tttccgctat acgcaggcat gactcgcgtc acctgatcac
      181 ggttggggaa atcccatggg cagcggtttt ccctggtgcc aagtcagtct tccatccttc
      241 agaggaagaa tacggattgg actttgtgag cgtccacttc tatcctgagt cgggaaagac
      301 tgaccaagca cttgctgcat tgttctccta tgacatcggt aagcccctcg ttgttgaaga
      361 gatgtttccg ctcaaatgca gtattgagga cttgcagagc tttgttgatg ggtcacgaga
      421 tcgagtgtca ggatactttg gattctactg gggtagaacc attgatgaat acagccaacc
      481 gcccgtgacc attcaggctg cgattctgaa ggcatggctt gagtactttc gcgaccacgg
      541 cagcacagtt cttcatccta cagaggccaa atgattggca caagaagaac cgataccagg
      601 agcagaagac aattgagcac ggcaccgact cgaaggaaat cgctgaagcg atatccacca
      661 ggaccgtaga ccatcatgtt ggtctgataa cccacaggag tcataaagca ggctgaggcc
      721 gcaagtgtga tcatcatcac aaatggcctc ggtgagactc ccatattgag cgcaatggcc
      781 actccaaagg gaaacatgag tgccgcggca gaattgttgc caaccaggga tgtgaagccc
      841 atagtcatta ggaatacgcc cgagagaact gcctttggcc ctccacggct gaagatatct
      901 gtaataatct gtgagacggt ggcaacacac ccggagttct caagaccttt tgcaataccg
      961 aacgcggcgc agatggtcaa caaggtctgc cactccagac tctgtctggc ctgagagact
     1021 gatatacatc ctgtggcaag cataagaccg gcaacgatga aggccgccat cacaatgctt
     1081 gttactcctg ttgccatcaa aacgatcagc accgccaaca gcgccagagc aatagcagcc
     1141 ttgtcatggc gaacaacgga ggaatcctca acaccactga caaggtagaa atcaggatcg
     1201 tttcgatgcg cagcgacaaa atgaggtccg gcttgcagca ggagcgtatc tccaggaagc
     1261 agaacaatat ctcccaccct gcctttcagg cgctctcccc ccctatgaac agctacaaca
     1321 gccgcgttgt atcgtgcacg aaagtttcct tcgcgaatgc tctgaccgac cataggagat
     1381 gtccgagaga ccaccgattc acaaagtagt ttctcgcggc gaacgtccat gcgagtctca
     1441 taactgtcat ccgcgatggg tacgaggcca gggatctttt ccaaatccac gatcgtcgag
     1501 atgatgcccg tgaaggtgag catatctccc tcaaagagag tctgctcggg accaacggga
     1561 gagatgatct ctcccttgcg gtctatttcg atcaggaaga gtccgtgtag atggcgtaga
     1621 ccggcttctt caactgtttt ggagatgaag cggtatccag attctacgcg aacattgatc
     1681 aggtagtctc tggaacaccc acgaatctgt tcgaggaaac ctttgcgtgt tggtatcaga
     1741 tggcgaccga caaagaagag atagagtact ccaacgaggg tacatgggac accaacaaaa
     1801 ctgagttcaa aaagccccat gggacgcaat gccaagcgca agcctggctc tgcgacatgc
     1861 acggcttcct ccatcagacc ctgaacgaca agatttgtgc ttgtgccaat aagcgtacag
     1921 gttccgccaa gcacggcgag gtaggagaga ggaagaagca agcgagaggg ggaaactttg
     1981 ttgttcttgc accaagtggt g
//
LOCUS       DAOQUC010000802         2000 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_72429_length_2000_cov_2.965553, whole genome shotgun sequence.
ACCESSION   DAOQUC010000802 DAOQUC010000000
VERSION     DAOQUC010000802.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2000)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72429_length_2000_cov_2.965553"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..340)
                     /gene="trpC"
                     /locus_tag="PKH07_12790"
     CDS             complement(<1..340)
                     /gene="trpC"
                     /locus_tag="PKH07_12790"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF012444.2"
                     /note="involved in tryptophan biosynthesis; amino acid
                     biosynthesis; converts
                     1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate to
                     C(1)-(3-indolyl)-glycerol 3-phosphate and carbon dioxide
                     and water; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol-phosphate synthase TrpC"
                     /protein_id="HNT35859.1"
                     /translation="MRPTEIIGQILARKRLEIDLLKASYSYEQLAQSASQVSPTRGFR
                     NCLTNKKPLAVIAELKKSSPSKGLLKADFEPVTLAIQYEQAGASAISVLTEQHFFLGS
                     PDYLKQVRQHV"
     gene            complement(337..1356)
                     /gene="trpD"
                     /locus_tag="PKH07_12795"
     CDS             complement(337..1356)
                     /gene="trpD"
                     /locus_tag="PKH07_12795"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015326112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HNT35860.1"
                     /translation="MESLIPFLKKVVSNQSLSAQESHEAMTIIMDGEATPAQIAGYLV
                     SLRMKGETPEEIVGAARAMREHATPVDVGDLFVVDTCGTGGDAKGTINVSTLAAFIAA
                     GAGVSIAKHGNRAASSRCGSADLLAGLGVNLDLAPEQVAECIKTIGIGFLFAPKLHQA
                     MKHAIGPRREMGIRTIFNVLGPLTNPANAKAQLMGVFDETLVEPLAEVLCLLGSEHAM
                     VVHGYGGYDEISSTGPTKIAEAKNGSIHLWTLDPKDLGVPYANSRDLLGGDVNQNIAI
                     TRRILDNELSPCLDLVVLNAAAAIVVGKKAEDMREGIEVARKSVASGSAREKLQQLIT
                     RSNAS"
     gene            complement(1499..1753)
                     /locus_tag="PKH07_12800"
     CDS             complement(1499..1753)
                     /locus_tag="PKH07_12800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35861.1"
                     /translation="MIEFSFSPILGRVTLGAAIWALLASVIIFHRYGAKIVGGLFFSC
                     SIVCVILHPTSEAVWVMLFSGLFFYFYGICKTRLSKPPMR"
     gene            complement(1750..>2000)
                     /locus_tag="PKH07_12805"
     CDS             complement(1750..>2000)
                     /locus_tag="PKH07_12805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009790407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35862.1"
                     /translation="GAIGLLAAGIVSFLIRLVSHAATNGEGVGATRYVAVGPWTLGAI
                     VLASFLVLWRHRSNIQRLRAGTEPKAGQRIEANTGGTP"
BASE COUNT          487 a          541 c          522 g          450 t
ORIGIN      
        1 agacgtgctg tcgtacctgt ttcagatagt ccggactgcc aaggaagaaa tgctgctcgg
       61 tcagaacgga gatggcggat gcaccagcct gctcatattg aattgccagc gtgaccggtt
      121 caaagtccgc tttcagaaga ccttttgaag gggaagactt cttgagttca gcaatcacag
      181 ccaggggctt cttgttggta aggcagttgc ggaagccacg tgtcggactg acctgtgatg
      241 cactctgtgc cagttgctca taggaatacg aggccttgag gagatctatc tcaagacgct
      301 tgcgggcaag tatctgtccg atgatctctg taggtctcaa ctggcgttgc tcctggtgat
      361 gagttgttga agcttctcgc gggcagaacc ggatgcgacg gatttcctgg cgacttcaat
      421 accttctcgc atgtcttcag cttttttgcc caccacgata gccgcggcgg cattcaggac
      481 aaccagatca agacagggag acagctcatt gtcgagtatc cttcgagtga tcgcgatatt
      541 ctggttgacg tctcccccaa ggagatctcg cgaattcgcg tagggaacac ccagatcctt
      601 tgggtcgaga gtccataaat ggatcgagcc attctttgcc tcggcgatct tggtcggtcc
      661 cgtgctggat atttcgtcgt agccaccgta tccatgaaca accatggcgt gctcggaacc
      721 cagcaagcaa aggacttctg ccaagggttc gaccagagtc tcgtcaaata ctcccatcaa
      781 ctgagccttt gcattggcag ggtttgtgag aggccccaag acgttgaaga ttgttctgat
      841 gcccatctca cgtctaggcc cgatagcatg cttcatggct tggtggagtt tcggagcaaa
      901 aagaaaaccg atgccaatag tcttgatgca ctcagcaacc tgctcgggag caagatcgag
      961 attgacaccg agtccagcaa gaagatccgc gcttccacag cgactggatg cagcccgatt
     1021 cccgtgtttt gcgatactga cccctgcacc ggctgcaata aaagccgcga gggtagaaac
     1081 attgattgtg cctttggcgt ctccccccgt tccacaggtg tccactacga agagatctcc
     1141 cacgtccacg ggcgttgcgt gttcccgcat agctcgggct gcgccaacga tttcctcggg
     1201 cgtttctccc ttcatgcgca gggagacaag atagcctgca atctgggcag gtgttgcctc
     1261 gccgtccatg atgatagtca tcgcctcgtg agactcctga gcactcagtg actgatttga
     1321 gacgactttc ttcaagaagg ggatcagaga ttccatcagt cggtgtcctc caaaacgcgc
     1381 agtacttatg tatgaatgca ttgtgacggg aatactctca gtacgtcaag ccagaagttt
     1441 cgtctctttc gaggcaatgc aagagcaaga acaagatcgg ctgacaggat tcgttgcatc
     1501 agcgcatggg tggcttgctc agtctagtct tgcagatgcc gtagaagtag aagaacaaac
     1561 cggagaacaa catcacccac accgcttcag atgttggatg cagaatcacg caaacgatgg
     1621 aacaactgaa aaacaggcca ccaacgatct ttgccccata gcgatggaag atgattaccg
     1681 aggcaagaag cgcccaaatt gcagcaccaa gcgtgacacg acccaggata ggcgagaacg
     1741 agaattcaat catggagttc cacctgtatt cgcctcaatc cgctgcccag cttttggttc
     1801 cgttccagcc ctcaatcgct gaatgttcga acgatgccgc cacagaacca gaaaagaagc
     1861 cagaacaatg gctccgagtg tccacggccc cactgcaaca tatctggtgg ctccgactcc
     1921 ttcaccgttc gttgcagcgt gggataccag acggatcaga aaactcacga ttcctgccgc
     1981 gagaagccct atagcaccac
//
LOCUS       DAOQUC010000803         2000 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_72430_length_2000_cov_2.964010, whole genome shotgun sequence.
ACCESSION   DAOQUC010000803 DAOQUC010000000
VERSION     DAOQUC010000803.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2000)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72430_length_2000_cov_2.964010"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            150..767
                     /locus_tag="PKH07_12810"
     CDS             150..767
                     /locus_tag="PKH07_12810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNT35863.1"
                     /translation="MTEWSAKKRIKHEETLDPHFGDEIANAPGGEAIFSCIQCGTCSG
                     TCPLSHSMDYTPRRIIAMTRAGFKDEVLRSNTIWICASCYSCSVECPRNIKVTDVMYA
                     LKQRALRARIYPKRFPIAVLAREFFQLVRMFGRNTESLLVTALFLKTNPLNLVKQSVL
                     GLKLLLKGRMPIIPLPERIKKRADLQKMLNAVERQEKVDGWHPVE"
     gene            764..1651
                     /locus_tag="PKH07_12815"
     CDS             764..1651
                     /locus_tag="PKH07_12815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit B family protein"
                     /protein_id="HNT35864.1"
                     /translation="MMKYLYYPGCSLKSTGRAYEESLLAVFGALGVQLEEIDDWNCCG
                     ATAYMAVDELKAFSLAARNLALAEKQVGAGNTAEVVAPCAACFLVQYKAQKYMKEYPQ
                     VEKEVRVALQSIGLHYDGKVNIRHPIEVLLRDIGVERIKEHVKRPLKGLKIACYYGCQ
                     LVRPYPVFDDPHNPKTKELLMQTLGAETVDWPLKTRCCGGSLTGTMGEIGLPLSHAIL
                     REAHRRGADVIATSCPLCQFNLECYQDQKTPGIEKTRIPVAFFTQLMGLAMGLSEKEL
                     GLQRLFVPVAPVLKSGERA"
     gene            1653..>2000
                     /locus_tag="PKH07_12820"
     CDS             1653..>2000
                     /locus_tag="PKH07_12820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013684225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide reductase"
                     /protein_id="HNT35865.1"
                     /translation="MAHLTPSHQPDVQIGVYVCHCGINIASKVDVPKTVEFAQTLPLV
                     RIAREYKFMCSDPGQEIIQKDIRELGINRVVVASCSPLMHEETFRKAVAEAGLNPFFF
                     QMANIREHVSWVTP"
BASE COUNT          476 a          505 c          550 g          469 t
ORIGIN      
        1 cctctgtcga acaggagcac aaggtttcgt ttttcccgtt actcttctca cacttgactc
       61 agtaggggtg acaccattat ggttctggga cgtttgcctg tttttgacga cgggacaacg
      121 tcctgagatt cattgcacga ggcagcccga tgactgagtg gagcgccaaa aagagaatca
      181 agcacgaaga gacgctcgat ccccactttg gagatgagat cgccaatgct ccaggtggag
      241 aggccatctt ctcctgtatt caatgtggaa cgtgcagtgg aacctgtccg ctgagccatt
      301 ccatggatta cacgccgcgt cggatcatcg ctatgacccg cgccggattc aaggatgagg
      361 ttctgcgcag caacaccatc tggatctgcg cttcgtgcta cagttgctcg gtggaatgcc
      421 ctcgaaacat caaggttact gatgtcatgt acgcgctgaa gcagcgagcg ctacgggcgc
      481 gcatctatcc aaagcgcttt cccattgctg ttctggcgcg cgaattcttt cagctggttc
      541 gaatgttcgg aagaaacacc gagagtctgt tggtgacagc gctatttctg aaaaccaacc
      601 ccttgaacct cgtcaagcag agtgtgctgg gactgaaact gctcttgaaa ggcaggatgc
      661 ccatcatccc gcttcccgaa cggatcaaaa aacgcgcgga cttgcagaaa atgctgaatg
      721 ccgtcgagcg gcaggaaaag gtggatggct ggcatcctgt tgaatgatga aataccttta
      781 ttaccccggt tgttccctaa agagtactgg cagagcgtat gaggaatctc ttcttgccgt
      841 ctttggcgct ttgggtgtgc aactggaaga gatagacgac tggaactgct gcggggcaac
      901 ggcatacatg gccgtggatg agttgaaggc cttttcactt gctgctcgaa atctcgccct
      961 ggcagaaaaa caagttggtg caggcaatac ggcagaagtc gttgctccat gtgccgcgtg
     1021 cttcctcgtt cagtacaagg cgcagaagta catgaaagag tacccacagg tcgaaaagga
     1081 agtccgtgtg gcgcttcaga gcatcggcct tcactacgac ggtaaggtca atatacgcca
     1141 tcccatcgaa gtactgctcc gagacatcgg cgttgaacgg atcaaggaac acgtgaagag
     1201 acctctgaaa ggccttaaaa tcgcctgtta ctatggatgt caactggtgc ggccgtatcc
     1261 tgtttttgat gatccgcaca accccaaaac caaggaactg ctgatgcaga ccctgggcgc
     1321 agagacggtg gactggcctt tgaaaacacg ctgttgtggt ggcagtctga cgggcaccat
     1381 gggagagatc gggttgcctc tgagtcatgc aatcctgcgt gaagcccatc gtcgaggagc
     1441 ggatgttatc gccacgagtt gtccactgtg tcaattcaac ttggagtgtt atcaggatca
     1501 gaagacgcct ggcattgaaa agacgcgcat tccggttgcc ttctttacgc aactcatggg
     1561 gctcgccatg ggtctttccg aaaaagaact tggactgcag cgtctgttcg taccggttgc
     1621 cccagtcttg aagagtggag agagggcgta atatggcaca tttaacacct tcccatcagc
     1681 cggacgttca gatcggtgtc tatgtttgcc attgtggaat caatatagcc tcgaaggtgg
     1741 acgttcccaa gaccgttgag tttgcccaga cattgcctct cgtccgaatt gcccgagagt
     1801 ataaatttat gtgttcggat ccagggcagg aaatcatcca gaaagacatt cgcgagttgg
     1861 gaatcaaccg tgttgtcgtg gcatcctgct cacccttgat gcacgaggag accttccgca
     1921 aggcagtcgc cgaagcgggg ctgaatccat tcttctttca gatggcgaac attcgagaac
     1981 atgtcagctg ggtcactccg
//
LOCUS       DAOQUC010000804         1997 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_72605_length_1997_cov_3.015963, whole genome shotgun sequence.
ACCESSION   DAOQUC010000804 DAOQUC010000000
VERSION     DAOQUC010000804.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1997
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72605_length_1997_cov_3.015963"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..403
                     /locus_tag="PKH07_12825"
     CDS             <1..403
                     /locus_tag="PKH07_12825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35866.1"
                     /translation="VPLAFDRHLVHFLAKLNPHSASIGIMIPHPGTEVHEMAVKGEGD
                     YVFSAASWSQFVKFGGGGLELKNLPRRSLEHLQAKAYLTFYMRNHRYWDFLKYAYSHR
                     RQAFVAARRLIPRIRKGQSVRATGNGAKTNG"
     gene            396..1142
                     /locus_tag="PKH07_12830"
     CDS             396..1142
                     /locus_tag="PKH07_12830"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HNT35867.1"
                     /translation="MDKDTILLSLVVPTYNEIANLDPLLSRLHSSLRDISYEIIFVDD
                     NSPDGTGKILDSLADEYHLTVIHRKGKLGLASAVLEGFRVASGSILGFMDADLSHPPE
                     TIPEMLRSILERQADIVIGSRLVDGGGVIGQWPQHRYLNSYIAEVIARPLTCVRDSMS
                     GFVLLKRSVIENVPLIPRGYKMGLEILVKGNYSKAQEVPILFDNRKRGSSKLDCRTRW
                     EYLVQIIDLYLYKARRVLKLSKRTKTVERN"
     gene            1148..>1997
                     /locus_tag="PKH07_12835"
     CDS             1148..>1997
                     /locus_tag="PKH07_12835"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNT35868.1"
                     /translation="MTNVTLVMPRFKYAAGDFSLGLASIASYLKQSLKDVQVHAVDAT
                     FDPDMEHIAEQLRQHPADITGIFMDTLMYRDALAVARIAKKHSPFVIVGGPHPSILPE
                     TVISDDSVDAVCIGEGEQTFSELVSSRKEGKSLEGVKGLWFKSDGAVVKNERRMPIED
                     IDTLPLPDIEIFDVERYIANFIQLDSYSPNLRGMSVIVSRGCPFGCSYCQPVLDTIFG
                     KRFRIRSPRKTVEDLKYLKQKYRLDGVYFQDDTLTVSRKWMAEFCALAVSERLDMVWA
                     CNTRADW"
BASE COUNT          486 a          489 c          552 g          470 t
ORIGIN      
        1 agtccccctc gcctttgacc gccatctcgt gcacttcctg gccaagttga atcctcacag
       61 tgcctccatc ggcatcatga tcccccaccc gggcacggaa gtgcacgaga tggcggtcaa
      121 aggcgagggg gactatgttt tctctgcagc atcctggagt cagtttgtga aattcggtgg
      181 cggcggactg gagttgaaga atcttccaag gcgcagtctg gaacatcttc aggcgaaggc
      241 atatctgacc ttctacatgc gcaaccaccg atactgggat tttctcaagt atgcctattc
      301 ccacagacgt caagcattcg tcgcagctcg cagattgatt cctcgcatac gaaaagggca
      361 gtctgtgcgt gcaacaggaa atggagcaaa gaccaatgga taaagacaca atcctgttgt
      421 ctttggttgt gccaacctac aatgagatcg ccaatctgga tcctctcctg tcgcgtttgc
      481 acagcagtct gcgcgatatt tcctatgaga ttatctttgt ggatgacaac agcccggatg
      541 ggaccgggaa gattctggac agccttgccg atgagtatca cctgacagtc atccaccgca
      601 agggtaagct gggcctggca tccgcagtgc ttgaagggtt tcgggtggcg agcggaagca
      661 ttcttggctt catggacgcc gacctgagtc accctccgga gacaatacca gagatgctcc
      721 ggagtatcct cgaacggcag gcggacatcg tcataggcag tcgccttgtg gacggtggtg
      781 gggtcatcgg gcaatggcca caacaccggt atttgaactc ttacatcgcg gaagtgattg
      841 cgagaccatt gacttgtgtc agggattcca tgtcggggtt tgttctgctg aaaagatcgg
      901 tgatcgagaa tgtgcctctg attccacgcg gttacaaaat gggcttggag attcttgtca
      961 aaggcaacta ctcaaaagcc caggaagtgc ctattctgtt cgacaatcgc aagagaggaa
     1021 gcagcaaact ggactgcaga acccgatggg aataccttgt acagataatc gatctgtatc
     1081 tctataaggc taggcgtgtt ctgaaactat caaaaaggac caagactgtg gaaaggaact
     1141 gaagaacgtg accaacgtca ctttggtgat gccacgcttt aagtatgccg cgggtgattt
     1201 ttcgctgggg cttgcatcca ttgcatctta tctgaagcag agcctgaagg atgttcaagt
     1261 tcatgctgtg gatgcaacat ttgacccaga catggagcac atcgcggaac aactgcggca
     1321 gcatcccgcc gatatcacgg gcatcttcat ggatactctg atgtaccgtg acgcgcttgc
     1381 ggtggcaaga atcgcaaaaa agcactctcc cttcgtcatc gttggaggcc ctcatccgag
     1441 tattcttcca gaaacggtga tcagcgatga cagcgttgat gcggtctgta ttggagaagg
     1501 ggaacagaca ttcagtgagt tggtttcatc tcgcaaggaa ggcaagagcc tcgaaggcgt
     1561 gaaagggttg tggtttaaga gcgatggggc agttgtcaag aacgagcgtc ggatgccgat
     1621 cgaggacatt gatactcttc ctcttccaga catcgagata tttgacgtgg agcgatacat
     1681 agccaatttc atccagctcg actcgtacag cccaaacctg cgtggaatgt ccgtcatcgt
     1741 gtcccgaggc tgcccgtttg gatgctctta ttgccagccc gtcttggata ctatctttgg
     1801 caagcgtttc cggatacgat ctcctcgcaa gacggtcgag gatctgaaat accttaagca
     1861 gaagtaccgg ctggacggtg tctactttca ggacgacaca ctcacggtat ccagaaaatg
     1921 gatggctgag ttctgcgctc tggcagtgag tgagagactg gacatggttt gggcgtgcaa
     1981 tacaagagca gactggg
//
LOCUS       DAOQUC010000805         1995 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_72706_length_1995_cov_3.765979, whole genome shotgun sequence.
ACCESSION   DAOQUC010000805 DAOQUC010000000
VERSION     DAOQUC010000805.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1995)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1995)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1995
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72706_length_1995_cov_3.765979"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..978
                     /gene="lnt"
                     /locus_tag="PKH07_12840"
     CDS             <1..978
                     /gene="lnt"
                     /locus_tag="PKH07_12840"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HNT35869.1"
                     /translation="QVIYGLWCLQAYEPHQSDPSIRVAMIQPVSPLKVLNSDGETKKE
                     IADTLLALSLAAVTQFDSTPDLLIWPEGAAPVSYQSPAFNSEFIDVIRDFHARSPVPL
                     FIQDIEFPNVSREPHERIRYYNHTSLIQSDGTLQAGYRKNCLLPMAEYLPWERSLPLL
                     RKCFPQTRSLLPGTEMKLIEGVGSPFVPLVCYELVFSDFVRRFVRKGGTYIVNVTNDR
                     WYGVDQQPYQHLAYAVFRAVENRRPVVRATNSGISAFVDSRGVIRKGERTHIMQRTAL
                     SATVYPSNTKSFYSRYGDLILKWGVTPLVLIVFTLRLRIAKKAKAKGIS"
     gene            975..>1995
                     /locus_tag="PKH07_12845"
     CDS             975..>1995
                     /locus_tag="PKH07_12845"
                     /inference="COORDINATES: protein motif:HMM:NF025182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4185 domain-containing protein"
                     /protein_id="HNT35870.1"
                     /translation="MRSTMTAVFVLAGLLMSTRAWTAVPAMSAVPDVSLEQLLLPQKR
                     GWLGADVDTSIPLTESRLLWLFGDTIVGTLTDQGARKGTMVRNSIGIQDLVGDSSAAV
                     QFYWDMRDNVPGSFFMAPNYDLDFCYWPCSGLMVDDELFVFCYKVVDGGGGVGAFAFK
                     VVGTILIRVANPLESPRQWVTTVTELPFGGEHQGFCSAVIQLDNFVYLIGFDDGVVAS
                     AQTRQAVLARIRVDDLKSNPTEGHFQYWVKSDRDEGQWSLDKSSLRPILPHGFAEGTV
                     YFDNSLNAFVMPIQTFFSSDISIVTAPTLTGPWSEPVKVYEVPETRDQRGIFAYAIKG
                     HPQLSQ"
BASE COUNT          460 a          520 c          514 g          501 t
ORIGIN      
        1 caagtcatct atggcctctg gtgccttcaa gcctatgagc cacatcagag tgatccatca
       61 attcgagttg cgatgattca gcctgtgtct ccactcaaag tgctcaattc cgatggagaa
      121 acaaagaaag agattgcgga cacgctgctg gctctttcac ttgcggcagt gacgcagttc
      181 gactcaacac ccgaccttct gatctggcct gaaggcgcgg ctccagtctc ctaccaaagt
      241 ccggcgttca attccgagtt cattgatgtc atcagggatt ttcatgcccg gtcgccggtt
      301 cccctgttta tacaggatat cgaattccca aacgtctccc gcgagcctca tgaacgcatc
      361 agatattaca accacacttc actgattcag tccgacggaa cactgcaggc tgggtaccga
      421 aaaaactgcc ttctcccgat ggctgagtac cttccttggg agcgttctct tcccttgctg
      481 cggaagtgct tccctcaaac tcgttcgctc ctgcctggca cagagatgaa actcatcgag
      541 ggagttggca gcccctttgt tccactggta tgctatgagt tggtgttctc tgactttgtc
      601 aggagatttg tcagaaaggg tggcacctac attgtcaacg tcacaaatga ccgatggtat
      661 ggcgttgacc agcaacccta ccagcatttg gcatatgccg tgttcagggc agttgagaac
      721 cgcaggccgg ttgtcagagc gaccaactca ggcatctcgg ccttcgtgga cagccgaggc
      781 gttatccgca agggcgagag gactcacatc atgcaacgca cagcactgag cgccacggta
      841 tatcccagta acaccaagag tttttatagt cgctatggtg accttatctt gaaatggggc
      901 gtaactccct tggtattgat cgtatttacg ttgaggcttc gcatcgcgaa aaaggcaaag
      961 gcgaaaggaa tatcatgaga tcaacaatga ctgcagtttt tgtgttggct ggcctgctga
     1021 tgagtacccg ggcatggaca gcagttccag ctatgtcggc agttccggac gtatcattgg
     1081 agcaactgct ccttcctcag aagcgaggct ggcttggagc cgatgtggac acttcaattc
     1141 cgctgacgga gagcagactg ctctggctgt tcggcgatac gatagtcggc actctcaccg
     1201 accaaggtgc gagaaagggt accatggtca gaaactcaat aggcattcaa gatttggtcg
     1261 gcgactcctc ggctgccgtt cagttctact gggatatgag agacaatgtt cccggttcct
     1321 tcttcatggc acccaactac gatcttgact tctgctattg gccttgttcc ggtttgatgg
     1381 tggacgatga actctttgtg ttttgctata aggtggtgga tggtggtgga ggagtcggcg
     1441 ctttcgcatt caaggtcgtc ggtacaatac tcatcagagt tgccaatcca ctcgagtccc
     1501 caagacaatg ggtcaccacg gtgacagaac ttcccttcgg cggcgagcat caggggttct
     1561 gttcggctgt aatccagttg gacaacttcg tctatctcat cggattcgac gatggagtcg
     1621 ttgcaagcgc acagacacgc caggcagttc ttgcacgcat ccgcgtggat gatctgaagt
     1681 caaatccgac ggagggacat tttcagtact gggtcaaatc tgacagggat gaaggtcaat
     1741 ggagcctgga caaatcttcc ttgcgtccga ttctgcccca tggatttgca gaggggacag
     1801 tctattttga caactctctg aatgcctttg tcatgccaat acagacattc ttctcatcgg
     1861 atatttcaat cgtgactgca ccaacgctca ctggcccttg gtcagaacca gttaaggtct
     1921 atgaagtccc tgaaacacgc gatcagagag gcattttcgc ctatgcgatc aagggacatc
     1981 cccaattgag tcagc
//
LOCUS       DAOQUC010000806         1993 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_72833_length_1993_cov_3.039216, whole genome shotgun sequence.
ACCESSION   DAOQUC010000806 DAOQUC010000000
VERSION     DAOQUC010000806.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1993
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72833_length_1993_cov_3.039216"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..583)
                     /locus_tag="PKH07_12850"
     CDS             complement(<1..583)
                     /locus_tag="PKH07_12850"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HNT35871.1"
                     /translation="MSETVIGAQLYTLRDFLKTPEDIAQTMKKVAKIGYRAVQLSGLG
                     KIDPKELRKIADGEGLEIAATHIGFNEMRDETQRVIDEHNTLGCKHSAIGGMPGEYRN
                     AEGYLRFAREATAVAQKLNAGGLTFSYHNHSFELQKFGDRTGLQILIEDSGSEVLFEI
                     DTYWIQHGGGDSTQWIDKVKKRIVLLHLKDMAVL"
     gene            complement(612..1532)
                     /gene="era"
                     /locus_tag="PKH07_12855"
     CDS             complement(612..1532)
                     /gene="era"
                     /locus_tag="PKH07_12855"
                     /inference="COORDINATES: protein motif:HMM:NF000908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HNT35872.1"
                     /translation="MGVDDQELRIESPDLRAGYVAVFGRPNVGKSTLVNALIGSKVSI
                     VSEQPQTTRDRVLCIYDDDDVQIVFLDTPGMHRPRDKMNDYMVHQARRCLQDADLSLM
                     MVEAQDAFGPGDRYLASWLKESKAPAILIINKIDLVSPSQLRSLESNLPEMDFWLERH
                     YISALNESGVSDLLCALKQRLPLGVRFFPEGLLTDRSERYLVGELVRESIFRLTREEV
                     PHSSAVVVDEFKEREDAKSFISATVYVETESQKGIIVGKEGQMIKRIGSTARQEMERL
                     IGRGVYLDLRVKVRKKWRKNPKDLREFGYQ"
     gene            complement(1605..>1993)
                     /locus_tag="PKH07_12860"
     CDS             complement(1605..>1993)
                     /locus_tag="PKH07_12860"
                     /inference="COORDINATES: protein motif:HMM:NF026049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35873.1"
                     /translation="EAGFLEAVDLADWLAAHRGLSFREAYRVAAASVEACRLQGRLTF
                     ISVNSCLPAGCRSLSEKEFAEITDLRRCLARRDQVGAPSPRQAKRSVARIRGYVKRQQ
                     RWASQAQERLTDAEERLHRAVAEILR"
BASE COUNT          471 a          574 c          446 g          502 t
ORIGIN      
        1 ccagaaccgc catatccttc agatgcagca ggacaatgcg ctttttcacc ttgtctatcc
       61 actgggttga atccccacca ccgtgctgaa tccagtaggt gtctatctcg aaaaggactt
      121 cagatccact gtcctcaatg agaatctgca gtcctgtacg atcgccgaat ttctgaagtt
      181 cgaaactgtg gttgtgatag ctgaaggtca gaccgcccgc attgagtttc tgagccactg
      241 cggtcgcttc gcgtgcgaaa cgtaagtaac cctcggcatt tcgatattca ccaggcatcc
      301 caccaatcgc agaatgcttg catccaagag tgttatgctc atcaatgact cgctgtgttt
      361 cgtcccgcat ctcgttgaaa ccaatatggg ttgcagcgat ttccagaccc tccccatcgg
      421 cgattttccg aagttccttt gggtcaatct tgccaagtcc agagagttga acggctcgat
      481 atccgatctt ggcaaccttc ttcattgttt gcgcaatgtc ttcaggtgtc ttgagaaagt
      541 ccctcagcgt gtacaactgt gctccaatga ctgtctctga catgatcttc ttgcctccac
      601 tttcgatgta tttattggta cccgaattct ctcagatcct tcgggttctt tcgccacttt
      661 tttcgcacct tgacccgaag atcgaggtac acgcccctgc caataagtct ctccatctcc
      721 tgtcgcgcag tgcttccaat gcgtttgatc atctggcctt ccttgccaac gataatccct
      781 ttttgagatt ctgtttccac gtagacggtg gcagagataa atgatttcgc atcctctcgt
      841 tctttgaact catccacaac caccgccgaa gaatgaggaa cttcttcacg cgtcagtctg
      901 aaaatgcttt cacggacgag ttctccgacc aaataccgct cacttcggtc agtcagaagt
      961 ccctcgggaa agaagcgaac gcccaatgga agcctctgtt tgagagcgca gagcagatcg
     1021 gacacgccag actcattcag agccgagatg taatgtcgct ccagccagaa gtccatctcg
     1081 ggcaggtttg actccaaaga cctcaattgg cttggactga ccaagtctat cttgttgatt
     1141 atgagtattg ctggagcctt tgactccttg agccaagacg ccagatagcg gtcaccaggt
     1201 ccaaaagcat cttgtgcctc aaccatcatc agtgacagat cggcgtcttg cagacagcgt
     1261 ctggcttgat gaaccatgta gtcattcatc ttgtcacggg gacgatgcat ccccggcgta
     1321 tccaagaaga caatctgtac gtcgtcgtcg tcatagatgc agagcactct atctcgagtg
     1381 gtttgcggtt gctcggacac aatcgacact ttgcttccga ttagtgcatt gaccaaagtg
     1441 gacttaccga cattggggcg accaaagacg gcaacataac cggcacgtaa gtccggagat
     1501 tcaattctca gttcctgatc atctacgccc aaggtataaa ttcccttcag aaatccaata
     1561 cagtattgcg attctgtccg tcaatggaga ccgccccaaa ccattcatcg aagtatctct
     1621 gcgacagccc gatgaagccg ctcctcggca tctgtcagtc tctcctgcgc ctgactcgcc
     1681 cagcgttgct gtctcttcac gtatccacga atccgagcca ctgagcgttt tgcctgtctc
     1741 ggcgaaggag caccaacttg gtcacggcgt gccaaacaac ggcgcaaatc cgtaatctct
     1801 gcgaattcct tctcagaaag acttcggcaa cccgcaggca gacaggagtt gacagagata
     1861 aaggtcagcc tgccctgcag ccgacaggct tcaaccgatg ccgcagcaac ccgataggcc
     1921 tctcgaaatg aaagtcctcg atgcgctgca agccaatcag caagatcaac tgcctcaaga
     1981 aatcccgctt ctg
//
LOCUS       DAOQUC010000807         1991 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_72942_length_1991_cov_3.002066, whole genome shotgun sequence.
ACCESSION   DAOQUC010000807 DAOQUC010000000
VERSION     DAOQUC010000807.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1991)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1991)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1991
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72942_length_1991_cov_3.002066"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1962)
                     /locus_tag="PKH07_12865"
     CDS             complement(<1..1962)
                     /locus_tag="PKH07_12865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35874.1"
                     /translation="MKGVFFEDDHVEQFDAILQALVGKGLASIQDISGLLDDLDLSVA
                     QDGRDAVRVLLRSLKRFDLPEFGTFSFANRQKFTTFLEDATAFFSYTSFREERNREKA
                     VKAIGEYVQLNPPGELFDPVDRRPFVSDEQFIEAIKSYVETRDRSIRDNLLDSDFVAV
                     RKIVNFRAPRDTKPAKETVRKLSGGPVEVVLEGLWATLADFKKLAYERGVYAHEVLRE
                     ISIESRLFKHDCDGSSPEERRERARAYLLRLIGGVDQYITKGIDSAFGDKDKVSVKSQ
                     LLHDGIECQPARTGEPCLQFSIAIVGEGFDESVVKTFSWRLPDIHPFRVAEELIQWAA
                     EVISNVENPCLPAFHAPYYEELLLAKDDEETRRVLLQCIHDESDGLSNLLEADGVDRK
                     DPLLRHLEALACEYDQFIQKAKSHGIHEALQSNWVLLRMKYEEACKAYLSDPSCGSSH
                     LAAMIFRAFMIITRRPGDSKHWMWESYERSGVVTVLHPALLEMLEAHTLYLLSAFAAI
                     AKRELHSPSTRSFREAVWQSHVDLAAIQMPLSGLIKDSNHILETDVRGDGLLHRIGSV
                     SHSDATLTTRLLLRYDAFDDDDISDSELFRTTGESKVITRILKDYYELHPHAFDGISI
                     AVYQNRDIQPIIAAVDEYLKYVCQERKPGI"
BASE COUNT          504 a          548 c          461 g          478 t
ORIGIN      
        1 aatgcccggc ttacgctcct ggcatacata cttgaggtac tcatccactg ctgcgatgat
       61 gggttgaatg tctctgttct gatacacagc tatgctgatt ccatcaaagg cgtgaggatg
      121 cagctcgtaa tagtccttca ggattcgcgt gatgaccttc gattctcccg tagttcgaaa
      181 caactctgaa tcgctgatat catcatcgtc aaatgcgtcg tagcgcagca ggaggcgcgt
      241 cgtaagagtc gcatcactgt gactgacaga accgattcga tgaagaagtc cgtcaccgcg
      301 aacatctgtc tccaagatat ggttcgaatc tttgatgagg ccggaaagag gcatttggat
      361 cgctgcgaga tcaacgtgac tctgccagac tgcttctcgg aaagaacgag tgctcggcga
      421 atgcagttcc cgtttggcaa tagcggcaaa cgcggacagc aggtacagtg tatgcgcctc
      481 cagcatctcc aagagtgcag gatgaagcac agtgaccaca ccggaccgct cgtaagactc
      541 ccacatccaa tgcttggaat ctccaggtct tcgcgtgatg atcatgaaag cgcgaaagat
      601 cattgctgca aggtgactac tgccacacga cggatcactg agatacgcct tgcatgcttc
      661 ttcatatttc attctcaaca agacccagtt actctgtagc gcttcatgaa tgccgtggct
      721 cttggctttc tggataaact gatcgtattc acacgcaaga gcctcaagat gacgcagcag
      781 cggatccttc ctatcaacac cgtctgcttc aagaagatta ctcaagccgt ccgactcgtc
      841 atgtatgcac tgcagaagga ctcgccttgt ctcctcgtca tccttggcaa ggagtagttc
      901 ttcgtagtaa ggcgcgtgaa atgcgggcaa acatggattc tcgacattgc ttatcacttc
      961 ggcagcccac tgaatgagtt cttcagcaac ccgaaaggga tggatatcag gcagcctcca
     1021 agaaaatgtc ttcacaacgg actcatcgaa accctctccc acaatggcga ttgaaaactg
     1081 caaacaaggt tcgccagttc ttgccggttg gcattcaatg ccatcatgga gcaattgcga
     1141 cttcacagag accttgtcct tgtccccaaa tgcagagtct attcccttgg taatgtactg
     1201 atcaacgccc ccaatgagcc tgagaagata ggctcgcgcc ctttctctgc gttcctcagg
     1261 agaggatcca tcgcaatcat gtttgaagag ccgcgattcg atgctgattt cccgcaacac
     1321 ctcgtgagca taaacgcccc gttcataggc aagtttctta aaatcagcaa gcgttgccca
     1381 cagaccttcc agcacaacct caacgggacc accgctcagc ttcctcacag tctctttggc
     1441 tggtttggta tctcttgggg cacgaaagtt gacaatcttc ctgacggcga caaagtcaga
     1501 gtcgagaaga ttgtcccgga tggatcggtc gcgagtttca acgtaactct tgattgcctc
     1561 aatgaattgc tcgtcactaa caaaaggtct tcggtcaacg gggtcgaaca actctccggg
     1621 cgggttcaat tgcacatact cccctatcgc tttgactgcc ttctcacggt tccgctcttc
     1681 acggaacgaa gtgtacgaga agaaagcagt cgcatcttcc aggaacgtag tgaacttctg
     1741 acggtttgca aaagaaaatg tgccgaactc cggcagatca aatcgtttca gactccgtaa
     1801 cagtactcta accgcgtctc gaccgtcctg cgcaacagag agatccagat catccaagag
     1861 tccgcttatg tcctgtatac tcgccagtcc cttgccaacc aacgcctgca ggattgcgtc
     1921 gaattgttca acgtgatcat cctcgaagaa aacacccttc aaagagcgaa tccattcctc
     1981 aaacgtcttg t
//
LOCUS       DAOQUC010000808         1990 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_72999_length_1990_cov_3.177778, whole genome shotgun sequence.
ACCESSION   DAOQUC010000808 DAOQUC010000000
VERSION     DAOQUC010000808.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1990
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72999_length_1990_cov_3.177778"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1990)
                     /locus_tag="PKH07_12870"
     CDS             complement(<1..>1990)
                     /locus_tag="PKH07_12870"
                     /inference="COORDINATES: protein motif:HMM:NF024909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HNT35875.1"
                     /translation="ELTSLWNSRYTSIWCGGWRGIDYIERLLRTDAFRRVTKDIWKMS
                     MGVSFLNPLLFALAALVTLPIIAHLIQRRRGRDMFFPTVRFLMVAQEEYRKRVRLQNR
                     HLLYLRASAFVVLTFALTQPVIRVAKTELASASSRTSAVILLDTSYSMGANDNGVVRM
                     DRAKQMVKDTLRIFREGDQVALITLSGRGNEVLVPLTPLSGLLEERIESVQTSSFSCD
                     ISGGLMQAEGLLKDAGDDLKEVYLISDLQQVAAQNIKESSPTGSKPINLHVLSVGPGD
                     AANTAVVDLTNNPAVVPEGGKATLRALVRHFGTEPQRRVLTQLVSGPGQTDKRNVDLN
                     PNVPEAVLFSKTYTATGLQTEKATVESDILAVDNERYLVVPVAQRSEALVLDGRPGAT
                     DGRGASFFVVKALEACGLGSAKRGSAAFDLRTMPVAGDVPPMDFRRYPLVIVCDVKGF
                     TADAARALVSFVESGGALLIFLGPNTEPSNINRTLVPKLLPYPLVGMKKMIGQDSQVS
                     YYFKTVDFQSPVFELFADLQNGDITLARFSNYQVIKADEAKTSSHPNANDDEGFARAS
                     VLAAFDSKDPAVISVDLGRGRALFCASSPDKVWTDLPLKSIFVPVIGEMVSHLTAAGD
                     RGFSFKPGDPVPITVRLRELAKQPVMLTVLAPDGQDYTV"
BASE COUNT          477 a          576 c          524 g          413 t
ORIGIN      
        1 aacagtgtaa tcctgcccgt caggcgccag aacggtgagc atgacaggct gctttgccag
       61 ttcccgaaga cgaacggtga tgggaaccgg atctcccggc ttgaaggaaa agcctcggtc
      121 tcccgccgcc gtgaggtgag agaccatctc gccaatgact ggaacgaaga tcgatttcag
      181 tggcagatct gtccaaacct tgtctggaga agatgcacaa aataaagcgc gtccgcgacc
      241 aaggtcaaca gagatcactg ccggatcctt ggagtcaaat gcagccagaa cggacgcacg
      301 agcaaagcct tcatcgtcgt tcgcattggg atgcgaactg gtcttggcct catccgcttt
      361 gatgacctga tagttgctga agcgtgccag cgtgatgtca ccgttctgca aatcggcgaa
      421 cagttcgaac actggactct gaaagtcaac cgtcttgaag tagtaagaga cttgggaatc
      481 ctgaccgatc atcttcttca tacccaccag aggataggga agcagtttcg gcacgagagt
      541 tctgttgata ttactcggtt cggtattggg acccaagaag atcaacagtg cgccaccact
      601 ctcaacaaaa gacaccagtg cccgcgccgc atccgcggta aatcctttga catcgcaaac
      661 aatgaccagg ggataccgtc ggaaatccat cggaggcaca tcgcccgcca cgggcattgt
      721 tcgaagatca aaggcggcag aacctcgttt ggcgcttccc aaaccacagg cctccaatgc
      781 cttgacgaca aagaaagacg ctccccgccc atcggtcgcg cctggacgac cgtccagaac
      841 aagcgcctcg gagcgctgtg caacgggaac cacaagatag cgctcattgt caacggcaag
      901 gatgtccgac tccacggttg ccttttccgt ctgcagccca gttgctgtgt acgtcttgct
      961 gaacaagacc gcttcaggga cattgggatt caagtccacg tttctcttgt ccgtctgtcc
     1021 cggtcccgaa accaattgcg tgagcaccct tctctgaggc tcagtcccaa aatgtctgac
     1081 cagagcgcga agagtggcct ttcctccttc gggcaccaca gctgggttgt tggtcaaatc
     1141 cacgaccgca gtgtttgccg catcaccagg ccccacggag agaacatgca ggttgatggg
     1201 cttgctgcca gtcggagaac tttccttgat gttttgtgcc gcaacttgct gaaggtctga
     1261 aatcaggtag acctctttca gatcgtcacc cgcatccttg agcagacctt cagcctgcat
     1321 caaacctcct gagatatcac aggagaaaga actcgtctga acgctttcta tgcgctcttc
     1381 caacagccca gagagcggcg tcaggggaac aagaacttca tttcctcggc cggacagggt
     1441 gatcagagca acctgatccc cttcccggaa gatgcgcaac gtgtctttca ccatctgttt
     1501 ggctcggtcc atacgaacca caccgttgtc gttagccccc atgctgtagg aagtgtcaag
     1561 caggatgact gcactggtgc gggacgacgc cgaagccagc tcagtcttgg caactctgat
     1621 gaccggctgc gtcagggcga aggtcagcac gacaaaagca cttgccctca ggtaaagcag
     1681 gtgccgattc tgcagtcgga cacgtttgcg gtactcctct tgtgccacca tcaggaaccg
     1741 gactgttggg aagaacatat cccggccacg tcgccgctga atcaggtggg cgatgatcgg
     1801 cagcgtcacc agcgccgcca gagcgaacaa caatggattc agaaaggata cacccatgct
     1861 catcttccag atgtctttcg taactcttcg gaaagcgtca gtccgaagaa gtcgttcgat
     1921 gtaatcaatg ccgcgccagc cgccgcacca gatacttgtg tagcgcgagt tccagaggct
     1981 cgtcagttct
//
LOCUS       DAOQUC010000809         1987 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_73162_length_1987_cov_3.781573, whole genome shotgun sequence.
ACCESSION   DAOQUC010000809 DAOQUC010000000
VERSION     DAOQUC010000809.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1987
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73162_length_1987_cov_3.781573"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1404)
                     /gene="rny"
                     /locus_tag="PKH07_12875"
     CDS             complement(<1..1404)
                     /gene="rny"
                     /locus_tag="PKH07_12875"
                     /inference="COORDINATES: protein motif:HMM:TIGR03319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HNT35876.1"
                     /translation="MLQMIMGISEMGWAMVACASLACLVIGYLFRKYTTESKTESAEK
                     LAARIVEEAEKEAQTKRREAVLEAKDHLFAEKTKFEQETREIRQELHGLEKKLSKRED
                     NLDKRSEAIEKKEREIQSQSQKLKNDEDKFQEKQKELDQLVAKQITQLEKLANLSTEE
                     AKQLLLSTLEQELVREQAVLIRRVTEETKEQAKKKAREIITTAIERCASDQVVESTVS
                     SVSLPSDEMKGRIIGREGRNIRSFEAITGINLVIDDTPEAVVLSGYDPVRREIARRTL
                     EKLIQDGRIHPGRIEELFNKTTKEVDEIIMEEGQQAAFDVGVHELHSEMIRFLGRLKF
                     RSSYGQNVLQHSIEVARLAAIMASELGADFELSKRAGLLHDIGKSVDFEMEGTHAKIG
                     ADLAKRYNESYKIVHAIAAHHEDEEAHTIEAVLVAAADAISAARPGVRRETLETYVKR
                     LEKLEEVAISVPVYVAPR"
     gene            complement(1392..>1987)
                     /locus_tag="PKH07_12880"
     CDS             complement(1392..>1987)
                     /locus_tag="PKH07_12880"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HNT35877.1"
                     /translation="ERCGDREIGRWRDGETGMLLREFLKIVQVPVVLDADGIYPLNSG
                     DVELVHSRGMRIITPHPGELSRFRGIPTAAIQQDRLGQALATAKENGITVVLKGAMTV
                     IAEPSGAVSINSTGNPAMASGGMGDVLTGVLGALLAQGLSLQASARLGVFVHGLAADR
                     LVARTGAMALMASQVSEELTGAFAQLKTWRLRKTCCR"
BASE COUNT          395 a          596 c          481 g          515 t
ORIGIN      
        1 ccgtggggcc acgtacaccg gcacgctgat cgcgacctct tcgagtttct ccagacgctt
       61 gacataggtc tcaagagtct ctcgacgaac tcccggtcgg gctgctgaga tggcgtctgc
      121 ggcagccacc aggacagcct cgatggtgtg agcctcttcg tcttcatggt gagcggcgat
      181 ggcatgcacg atcttgtaag attcgttgta ccgtttagca agatcggcac cgatcttggc
      241 gtgagttcct tccatttcaa agtcaaccga tttgccgatg tcgtgcaaca agccagcgcg
      301 cttgctcagc tcgaagtctg cacccagttc actggccata atcgcagcca gtctggcgac
      361 ttcgatggaa tgctgaagca cattctgacc ataggaagaa cggaatttca agcgacccaa
      421 gaagcggatc atctctgagt gcagctcgtg tactccgacg tcgaaagccg cctgttggcc
      481 ttcttccatg ataatctcgt ccacttcttt ggtggttttg ttgaagagtt cctcgatccg
      541 acctggatga attctgccat cttgaatgag tttctcgagt gttctgcggg caatctcgcg
      601 acgtacggga tcgtagcccg agagaacaac cgcctccgga gtgtcatcaa tgacgaggtt
      661 gattcccgtg atggcctcaa aagagcggat gttgcggcct tctcgaccga tgattcgacc
      721 cttcatttcg tcactgggca gcgagaccga gctgacggtg gactccacaa cctgatcgga
      781 ggcgcaacgc tcaatcgccg ttgtaatgat ctctctggcc ttcttcttgg cctgttcctt
      841 ggtttcctcg gtgacgcgcc gaatcagaac tgcctgctcg cgcaccagtt cctgctccaa
      901 cgtgctcagg agaagctgct tggcctcttc cgtagaaagg ttggcgagtt tctccagttg
      961 ggtgatctgc ttggcgacaa gttggtctag ctccttctgc ttctcctgga acttgtcttc
     1021 gtcgttcttc aacttctgtg actgtgactg aatctcacgt tcctttttct caattgcctc
     1081 cgaacgcttg tcgagattgt cttcacgctt gctcagtttc ttctccagac catggagttc
     1141 ctgacgaatc tcacgtgtct cctgctcgaa cttggtcttc tcggcgaaga ggtggtcttt
     1201 tgcttccaga acagcctcgc gcctcttggt ctgagcttcc ttctcggcct cttctacgat
     1261 tcgagcggcc agtttctcag cactctcggt cttggattct gtcgtgtact tccgaaacag
     1321 atagccaatc accaggcacg caagggaagc gcaagccacc attgcccagc ccatttccga
     1381 tatgcccata atcatctgca acatgtcttt cttaacctcc aagtcttcag ttgtgcgaag
     1441 gctccagtca gctcctcaga gacctgagag gccatgagag ccatcgctcc agtccgggcg
     1501 accagtctat ccgcggccag cccatgtaca aaaactccca atcttgccga tgcctgaagg
     1561 gataagccct gcgccagaag cgctccgagc acccccgtca gaacatcacc catacctcct
     1621 gatgccatcg ccggatttcc ggtggagttt atcgaaacag ccccagaggg ttccgcgatg
     1681 actgtcattg cccctttcag gacaacggtg attccatttt cttttgcagt tgccaatgct
     1741 tgcccaagtc tgtcctgctg aatggctgct gtcggaatgc ccctgaatcg gctcaactca
     1801 cccggatggg gtgtaatgat tcgcattcct cttgaatgaa ccaactccac atcaccggag
     1861 ttcagaggat agatgccatc tgcgtccagg acaacaggta cttggacgat cttgaggaac
     1921 tctcgaagaa gcatccccgt ctccccatct ctccatctcc ctatctccct atctccacat
     1981 ctttcca
//
LOCUS       DAOQUC010000810         1987 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_73169_length_1987_cov_3.420807, whole genome shotgun sequence.
ACCESSION   DAOQUC010000810 DAOQUC010000000
VERSION     DAOQUC010000810.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1987
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73169_length_1987_cov_3.420807"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="PKH07_12885"
     CDS             <1..253
                     /locus_tag="PKH07_12885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35878.1"
                     /translation="REVLMKLYGVGYDVQNNVIFGSDCRASDYNVNWVRQWINRDNAI
                     YDDLQIEKGILDRIYSANLLRFLGLSSDDVPRKILRIGE"
     gene            complement(256..1317)
                     /locus_tag="PKH07_12890"
     CDS             complement(256..1317)
                     /locus_tag="PKH07_12890"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNT35879.1"
                     /translation="MTSKERVLAYLERRIPDRVPTSYRATSEVTHKLLAHFGTDDFQV
                     VRRALGIDGFFDVSPVYVGPDLHRFGDGTWEDFWGTRYKNIQYATGCYAEKCFFPLQG
                     ARTAEQLDRYRWPSLDWWDFSCVSEQAQRAQDQVVRGGVYSLFEQFNALKPLDECLTD
                     ILVEPEFVLDVFVRLHGFWMQYLERLFQAAEGAIKMLFISDDVGMQDRMMMRPDVWRG
                     FFPPFYRELTAMAHRYGVSVAYHSDGSIDPIIPDLIEAGADILEPVQHVCPGIDREHL
                     KAAYGDRLLFMGAVENQRVLPFGTVEEVREETRECLRLLAKGGGYMLSSCHNLQPVTP
                     VENIIAMYQVVQEEGHLYF"
     gene            complement(1327..>1987)
                     /locus_tag="PKH07_12895"
     CDS             complement(1327..>1987)
                     /locus_tag="PKH07_12895"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNT35880.1"
                     /translation="FGTLGGIASLYISIKERRHINWRELRRVLLWCAIGFPVGVYGFH
                     KLPVNRLNLFLAVFIVIVSVHGFWKTYTKRASRPWPWLFGRILLILGGVIHGAFTTGG
                     PAVIAYSEPILKDKHEFRATMMLIWGILNPIFVTIYWLSPSRQIEVFWLTLACIPSIF
                     IGIRTGQFLHHRVSERGFRLAVFTLLFLSGISRLMPNDHPAKPEEISHTAPVESLQTV
                     D"
BASE COUNT          507 a          534 c          515 g          431 t
ORIGIN      
        1 cagggaagtg ctgatgaaac tttatggggt tgggtacgat gttcagaaca atgtcatttt
       61 tggaagcgac tgccgggcgt ctgactacaa tgtcaattgg gtgcgtcaat ggatcaaccg
      121 tgacaacgcc atctatgatg acctacaaat cgaaaagggc attctagaca gaatctactc
      181 cgccaacctg ctgcggtttc ttggtctctc ctcggatgat gttccacgca agatactcag
      241 aattggtgag tgaagctaga agtacagatg cccctcttcc tgaacgacct gatacatggc
      301 gatgatattc tccactggtg tcaccggctg aaggttgtgg caactggaga gcatatagcc
      361 gcctcctttg gcgagaagtc tcaggcactc gcgagtttcc tctcgaactt cctcgaccgt
      421 tccaaaaggc agaacgcgct ggttttcaac cgcacccatg aaaagcaggc ggtcgccgta
      481 cgccgctttc agatgctctc gatcaatgcc cggacaaacg tgctgcaccg gctccagaat
      541 gtcagccccc gcctcaatga gatctgggat gatcgggtca atactcccat cactgtggta
      601 ggcaacgcta acgccgtacc ggtgagccat ggctgtcagc tctcggtaga atggggggaa
      661 gaagcctctc cagacatcgg gacgcatcat catgcgatcc tgcattccga cgtcgtcgct
      721 gatgaacagc atcttgatgg caccttctgc tgcctgaaag agccgttcaa gatactgcat
      781 ccagaatcca tgcaatcgga caaacacatc cagaacaaat tcgggctcga ctagaatatc
      841 ggtcaggcat tcatccaacg gcttaagagc gttgaactgc tcaaaaagcg agtagacgcc
      901 cccgcgaacg acctgatcct gtgcccgttg agcctgctcg gaaacacaac taaaatccca
      961 ccagtccaga ctcggccagc gatatcgatc cagttgttct gcagttcggg caccttgcag
     1021 tgggaagaag cacttctctg cgtaacaacc ggttgcatat tggatattct tatatcgggt
     1081 tccccagaag tcttcccagg ttccatcacc aaagcgatgc aagtcgggtc ccacatagac
     1141 tggactgaca tcaaagaagc cgtcaattcc caacgcgcga cgaaccactt ggaagtcatc
     1201 tgtgccaaaa tgcgccagga gtttgtgagt cacttctgac gtagctcggt aactcgtggg
     1261 cacccggtcg ggaatccgcc gttccagata ggcaagaaca cgttccttgg aagtcatacc
     1321 ccacctctag tccaccgtct gcaagctctc cacaggcgcg gtgtgagaga tttcctcagg
     1381 ttttgccgga tgatcgttgg gcatcaatct ggatatccct gagagaaaga gcagagtgaa
     1441 gacggctagt cgaaagcctc gctcagagac cctatggtga agaaactgac cagtccgaat
     1501 cccgatgaaa atggacggga tacaggcaag agtgagccaa aagacctcga tctggcggct
     1561 tggactgagc cagtagatcg tgacaaaaat agggttcaga attccccata tcagcatcat
     1621 ggttgccctg aactcgtgct tgtctttgag aattggctcg ctgtaggcga tcaccgctgg
     1681 ccccccagta gtgaacgctc catggatgac tcctcctagg atcaagagga ttcgaccaaa
     1741 aagccaaggc catgggcgtg aggctcgttt tgtgtaagtc ttccagaagc cgtgaaccga
     1801 gacgatcacg atgaagaccg ccagaaacag gttcagtctg tttactggga gtttgtggaa
     1861 tccgtagacg ccaacaggaa aaccgatggc acaccagagc agaacgcgcc tcagttcacg
     1921 ccagtttatg tggcggcgtt ccttgattga gatgtaaagg gacgcaatgc ctccgagagt
     1981 tccaaac
//
LOCUS       DAOQUC010000811         1985 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_73283_length_1985_cov_3.582902, whole genome shotgun sequence.
ACCESSION   DAOQUC010000811 DAOQUC010000000
VERSION     DAOQUC010000811.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1985)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1985)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1985
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73283_length_1985_cov_3.582902"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..926
                     /locus_tag="PKH07_12900"
     CDS             <1..926
                     /locus_tag="PKH07_12900"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35881.1"
                     /translation="AEFRSAWDAIRWYFTQPITFWAIVLGTLVTQVGFFCVDQVTLQR
                     FFSSKGAREASRSFVLNAIGLSTVGLMLSFLGLGLFAFYRGTLPDAVAQKGWDGVFPY
                     FTAHEIWPGVAGLIIAALFAATMSSVDSGINSVSTAVLVDFHQRLILGQRYPADTEGN
                     VEQDRRQLVMARWLTIFFGLVATGLACFVKEIGDSIIKITNIIVNNFCGPMLGIFLLG
                     IFSRRASPWGVLISPVLGVLTAFVMTAPLKKFGLPWEAGVAPFWASVIGLVVTLILGY
                     AISLVENLISGCTPEHKLRGVTWAKRFDESD"
     gene            938..>1985
                     /locus_tag="PKH07_12905"
     CDS             938..>1985
                     /locus_tag="PKH07_12905"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNT35882.1"
                     /translation="MTVGRKARICRLMLFAVVFSAYQAVGAQLQVIEPLEPPVLLLGS
                     ALPMRQVLYVTLKNVGEEAVSESRLIAHSDQLVSAISEPIVCAASQAVMCSVLLLPGD
                     GLTVGDPVSIHVDLLDGEGQRTRLGSAETTTALCHPLLCQRSAASADDFFIVQVSDPH
                     IIKHDSTPGNVKTADRLEQAVSEINAMNPAPDFVVVTGDILLDNAVGYPLYEEIISTL
                     NVPWLTAFGNHDKPAGLAHAARVFSEWGHSEYYAFVHKGYWFFVLDGVQDIRPNFAEF
                     TPTQLQWLQVLLEQTTMDRPQTFFLHHDLFSGRGVKDIAPAQAVLERFETEKWFFSGH
                     WHADCFVRRGTQRHI"
BASE COUNT          391 a          507 c          558 g          529 t
ORIGIN      
        1 atgcggagtt tcgttcggcc tgggacgcca tccggtggta cttcacacag ccgatcacgt
       61 tctgggcaat tgtccttgga acattggtca cccaagtcgg tttcttctgt gtggatcagg
      121 tgacgcttca gcgcttcttc tcgtcaaaag gagctcgtga agcatctcgt tcttttgtgc
      181 tgaatgccat tggactgagc acggttggtt tgatgctctc ctttctcggt ctgggtctat
      241 tcgcgttcta tcgagggacg ttgcccgacg cagttgctca gaagggatgg gacggcgtct
      301 ttccgtactt cacagcacac gaaatctggc ctggtgtggc tggactgatc atcgctgcgc
      361 tcttcgctgc gaccatgtcc agcgtggatt ccggcatcaa ctccgtcagc acggctgtgt
      421 tggtggactt ccatcagcgc ttgattctcg gacagcgtta tccggcggac acggaaggca
      481 acgtcgagca agatcgccga caacttgtca tggcgcggtg gctcaccatc ttcttcggtc
      541 tggtcgcaac ggggcttgcc tgctttgtga aagagatcgg tgacagcatc atcaagataa
      601 caaacatcat cgtgaacaat ttctgtggtc cgatgctggg catcttcttg ctggggatat
      661 tctcaaggcg cgcctctccg tggggcgttc tcatttcacc ggtgcttgga gtgctgacgg
      721 cgttcgtcat gaccgcgcca ctgaagaagt ttggcttgcc atgggaagca ggtgtcgctc
      781 ctttctgggc ttcggtcatt ggcctggtgg tgactttgat tcttgggtat gctatcagcc
      841 ttgttgagaa tctcatctca ggctgtacgc ccgaacacaa actgcgaggg gtcacgtggg
      901 cgaagcggtt cgatgaatct gactgaggac caatgagatg actgtaggac gaaaagcacg
      961 catttgtcgt ttgatgcttt ttgctgttgt gttctccgcg tatcaggctg tgggagcgca
     1021 actgcaagtt attgaaccac tggagccacc ggtcttgctc ttgggttcgg ctcttccaat
     1081 gcgacaggta ctttatgtca cgttgaagaa cgttggagaa gaggcggttt ctgaatcccg
     1141 actgattgcg cattcggatc agcttgtctc ggcgatctca gagccgattg tctgcgctgc
     1201 gagccaagcg gtcatgtgct ctgtcttgct tctgccagga gacggcctta cggtcggtga
     1261 tcctgtttca atccacgttg atcttctgga cggagaaggt cagagaactc gactgggctc
     1321 tgccgaaaca acgacggctt tgtgtcatcc gcttttatgc cagcgctccg ctgcgtcagc
     1381 ggatgatttt ttcatcgtcc aggtcagtga ccctcacatc atcaagcacg acagcactcc
     1441 cggaaatgtc aagactgccg atcgtcttga acaggcggtc tctgagataa acgccatgaa
     1501 tccagcaccc gattttgtcg tggtgacagg agatatcctt ctcgacaatg ctgttggtta
     1561 cccattgtac gaggagatca tctcaacact gaacgtgccg tggttgacgg cttttggcaa
     1621 ccatgacaaa cctgccggtc ttgcccatgc tgcgagggtc tttagcgaat ggggacactc
     1681 ggagtactac gcctttgttc acaaaggata ttggttcttc gtcctggatg gtgttcagga
     1741 tattaggcct aatttcgcgg agtttacgcc cacgcaactg cagtggctcc aggtcttgct
     1801 tgagcagaca acaatggata ggccccaaac gttttttctc catcacgatc tattcagcgg
     1861 tcgtggcgtg aaagacatcg ctcctgcaca ggcagtgctc gagcgattcg agacagaaaa
     1921 atggtttttc agtggacact ggcatgcgga ctgttttgta cgccggggaa cgcaacgtca
     1981 cattg
//
LOCUS       DAOQUC010000812         1984 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_73349_length_1984_cov_3.638154, whole genome shotgun sequence.
ACCESSION   DAOQUC010000812 DAOQUC010000000
VERSION     DAOQUC010000812.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1984)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1984)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1984
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73349_length_1984_cov_3.638154"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1482
                     /locus_tag="PKH07_12910"
     CDS             <1..1482
                     /locus_tag="PKH07_12910"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNT35883.1"
                     /translation="YSGFEERRSEMTQKRSLSLLWICFSLALISFGAGCARIPTHPSA
                     LRYPELNVEYPKPERVVLDNGLVIYLLEDHEVPIVDGFLTVRGGSIYDPPEKIGLASM
                     TGAVMRTGGTPDMTGDEIDEELELLAASVETSFSREEANASFSTMAKDLDRVLQLYAD
                     VILRPAFREDKIELKRREMLEEIRRRNDDPGRIASREFGFLVYGKENPWARVPSRSDV
                     LSLTQQDLVEFHRRSFRPDQAILALAGDFSRNEMLEKIKKIFGDWKPSGEPFQKLEPI
                     RDDAPRVGVHIVRRETEQVNIRLGHLGISRHDPDRFAVLLLNYILGDGGFNARLIQTI
                     RSDLGLAYVVWGSIGHGTDRGAFTVVAETKSESTGLAVEKMIEIIQGMCNELVSEEDI
                     SRAKESLINQFVFLYDSSWGIVRQYASLEHYGYAEDYLDKYLDRLKAVTREDIQNAAR
                     KHLHPDSLVILAVGNPDTSETPLSKFGTVSEITLDAPEAQETP"
     gene            1479..>1984
                     /locus_tag="PKH07_12915"
     CDS             1479..>1984
                     /locus_tag="PKH07_12915"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HNT35884.1"
                     /translation="MTFAEAEEYLSSLISYEKYGRAKYGQQTFSLTVFRHILERLGNP
                     ERQLRAIHVAGTDGKGSVCQYLTCILQQAGFSVGTYTSPHLQTVRERIQINRVPIDEL
                     DFANQVERVRPILDSLPDALPAETPEGERNPPSFFEALTAMAFLHFADKQVDYAVIEV
                     GLGGRLDAT"
BASE COUNT          453 a          484 c          553 g          494 t
ORIGIN      
        1 tactcaggct ttgaagagag gcggagcgaa atgactcaga agagaagtct ttccctgttg
       61 tggatctgtt ttagtcttgc actgatatcc tttggtgccg gctgtgcgcg tattccgaca
      121 catccttcgg cactgcgcta tcccgaactg aatgtcgagt acccaaaacc ggaacgggtt
      181 gtgctggata acggtctggt gatctatctg ctcgaagatc acgaagtgcc tattgtggac
      241 ggttttctga cggttcgcgg cggctccatc tacgacccac cggagaagat cggtctggca
      301 tccatgaccg gcgctgtcat gagaaccggt ggcacaccgg atatgacagg agatgagata
      361 gacgaagaac tcgaactgct ggctgcttcc gtcgaaacat ccttttcccg agaggaggcg
      421 aacgcatcct tctcaacgat ggccaaggac ttggatcgcg ttcttcaact gtacgccgat
      481 gtgatcctgc gtccggcgtt tcgtgaagac aagattgaac tgaaacgaag agagatgctg
      541 gaagaaatac ggcgtcgaaa tgatgaccct ggaaggattg ccagtcgtga attcggcttt
      601 ctggtctatg gcaaggaaaa cccctgggca cgtgtgccct ctcggagcga tgtcttgtcg
      661 ctgactcaac aggatttggt tgagtttcat cgaagatcgt ttcgaccgga tcaggcgatt
      721 ctcgcgctcg cgggggattt ctcacgcaat gagatgctcg agaagataaa aaagatcttc
      781 ggtgattgga aaccgtcggg tgaaccattc caaaagctgg agccgattcg cgatgatgct
      841 cctcgggttg gcgttcatat cgttcgaagg gaaacagagc aggtcaacat tcgactggga
      901 catttgggaa tctctcgaca cgatcccgac cgatttgctg tcctgcttct caattacatc
      961 cttggcgatg gtggtttcaa tgcgcgcctg attcaaacca tccgctccga tcttgggctg
     1021 gcctatgtgg tgtggggttc gattggtcat gggacagatc ggggtgcatt caccgttgta
     1081 gcggagacaa aatctgagtc caccggtctt gcggtggaaa agatgatcga gataatccaa
     1141 gggatgtgca acgagctggt ttctgaagag gatatttcac gagcgaagga aagcctcatc
     1201 aaccagttcg tgttcctcta tgattcttcc tggggtattg tgcgacaata cgcttccttg
     1261 gagcactatg gctacgcgga ggattatctc gataaatacc tcgaccgttt gaaagcggtc
     1321 acgcgtgaag acattcagaa cgcagcgcgg aaacatctgc atccggattc tctggtgata
     1381 cttgctgtgg ggaatcccga cacttctgag acacctctct cgaagttcgg aacggtttcc
     1441 gagatcacct tggatgcccc tgaagcacag gaaactccat gacgtttgct gaagcggagg
     1501 aatatctctc ttctctgatc tcgtatgaga aatacggtcg tgccaagtac gggcagcaga
     1561 ctttctcgct cacggttttt cgacacattt tggagcgctt ggggaatcca gaaaggcaac
     1621 tgcgcgccat tcatgttgcc ggcacggacg gaaagggatc ggtctgtcag tatctcacct
     1681 gtattcttca gcaggccggt ttctccgtcg gcacctatac gtctcctcat ctgcagactg
     1741 tgcgagagcg cattcagatc aatcgtgttc caatagacga attggacttt gcgaatcagg
     1801 ttgagagagt tcgaccaatt ctcgattcgc ttccggacgc gttgcctgct gagacaccag
     1861 agggcgaacg aaatcccccg agtttttttg aggctcttac agcgatggcg tttcttcatt
     1921 tcgccgacaa gcaggtggac tatgcagtga ttgaagtcgg tctcggtggt cgcttggacg
     1981 caac
//
LOCUS       DAOQUC010000813         1984 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_73354_length_1984_cov_3.241576, whole genome shotgun sequence.
ACCESSION   DAOQUC010000813 DAOQUC010000000
VERSION     DAOQUC010000813.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1984)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1984)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1984
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73354_length_1984_cov_3.241576"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..436)
                     /locus_tag="PKH07_12920"
     CDS             complement(<1..436)
                     /locus_tag="PKH07_12920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35885.1"
                     /translation="MNATNASRGTWGIRLLIRLLSITLGVLLFWLLGFLVNDIESLRG
                     PDYQTIEAKHLDSSLVERKEKLLAEIADLDRQITNKREEQRLAGDSSQNLQRTINQLL
                     DIQKMSMEKDISLADSDRENISASLKHFLESQTVYHIRNNPAA"
     gene            713..1030
                     /locus_tag="PKH07_12925"
     CDS             713..1030
                     /locus_tag="PKH07_12925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35886.1"
                     /translation="MTQESVISAGDGNNCDPATFEMTRLALFNQGKDTARLSHDLNNR
                     LFAVSGRVELLRDALNLGDEAKVKRLLENIFAELSQLGEKVHELNEVAHRYDLLAGID
                     SGR"
     assembly_gap    1174..1183
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1184..1486)
                     /locus_tag="PKH07_12930"
     CDS             complement(<1184..1486)
                     /locus_tag="PKH07_12930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35887.1"
                     /translation="MQKSVGLVLFSTLLAVFCACHQKSDPYYYGPGFLYATGHADVKG
                     DQPQSIAEARSSALVDARAKLEKKVLAFQMDQKRTVRTIIKENPDMAPKLEKMIRKS"
     gene            complement(1517..>1984)
                     /locus_tag="PKH07_12935"
     CDS             complement(1517..>1984)
                     /locus_tag="PKH07_12935"
                     /EC_number="2.1.3.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019398074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HNT35888.1"
                     /translation="KITLAAERSLEQRIPFVVISAGGGGARMHEGMLSLMQMAKTSAA
                     VANLRKASIPYISVVTNPTMGGVAASFASLGDINLAEPKALLGFAGPRVIQQTIGERL
                     PPGFQTSEFLLEHGMLDAIVSRQEMRNTLALILRCLLNQCRPKEAPVTIAATT"
BASE COUNT          459 a          483 c          525 g          507 t
ORIGIN      
        1 tggcggcggg attattcctg atgtggtaga cggtttgact ttcgaggaaa tgcttcaggc
       61 tggccgagat gttttccctg tcggagtctg ccagtgagat atctttctcc atgctcatct
      121 tctgaatatc aaggagttga ttgattgtcc gctgcaggtt ctgggaactg tcccctgcaa
      181 ggcgctgttc ctcgcgtttg ttggtgatct gtctgtccaa gtccgcaatt tccgcgagga
      241 gtttctcttt ccgttcgacc agagaagaat ccagatgctt ggcttcaatc gtctggtaat
      301 ccgggccacg gaggctctcg atgtcattga ccaggaatcc gagcaaccag aacagcagca
      361 cgccaagtgt gatgctcagc aggcggatca agagacgaat tccccaggtt cctcgacttg
      421 cgtttgttgc gttcatggac gactccttgc gaccgaagga agataacctt ccttcctcgt
      481 catatccgta ttggcatttc cacctgagaa tacgcttcga acaggtacga tcaagtctgt
      541 ctcagagcac tcttgcatga cgtgatcgtg atcttcaaaa gcagcgtatt ccgtcttgct
      601 gttcaagtca aatgcgcatt tatgcagcca gaatctgtcg cagcagagtt ttcccttgat
      661 tggcagaaag caagtcatgt aggatggcat cagaaagtga gggatggtgg agatgactca
      721 agaaagtgtg atttccgctg gcgacggcaa taactgtgat ccagcgacat ttgagatgac
      781 gaggcttgct ctctttaacc aagggaagga taccgcgcgg ctgagtcacg atctgaacaa
      841 caggctcttt gccgtttctg ggcgagtcga gttgcttcgc gatgctctta acctgggaga
      901 cgaggcaaaa gttaagcgtc ttctggagaa tatctttgca gaactgtcgc agttaggaga
      961 gaaggttcac gaactcaatg aggttgcgca tcgctatgac cttcttgcgg ggatagactc
     1021 tgggagataa acaagctcat tggaggaaac agacttcttc agagccagga gcctcttgtg
     1081 agatctagcg ggtgatcttc tccaactgct tcaggttgaa ctccagttcg agttccagac
     1141 gttgctcctg tgtcctctca gagacgacct tggnnnnnnn nnnggatttg cggatcatct
     1201 tctccagttt cggagccata tccgggtttt ctttgatgat ggtgcgaaca gttcgtttct
     1261 gatccatttg aaaggcaagt accttcttct caagtttggc tctcgcgtca acgagagcgc
     1321 tggaacgagc ctctgcaatg gactgtggct gatcaccctt aacatcggca tgacctgttg
     1381 catagagaaa tccgggacca tagtaatagg gatcggattt ctggtgacat gcgcaaaaga
     1441 ctgcgagaag agttgagaaa aggacgagac caacggactt ttgcattgtg cgatctcctt
     1501 ttggcaaaaa ctgaccttaa gtggtcgcgg ctatcgtaac cggtgcctct ttaggtctgc
     1561 attgattgag cagacaacgc agaatgagtg ccagtgtgtt gcgcatctcc tgacgcgata
     1621 cgatggcgtc caacatacca tgttcaagaa ggaattcgga agtctgaaaa cccggtggca
     1681 gccgttcccc aattgtctgc tggatgacac gaggccctgc aaaacccagt agcgctttcg
     1741 gctctgccag atttatgtca ccgagcgatg cgaaacttgc cgccactccg cccatcgttg
     1801 gattcgttac gacggaaatg taaggaatcg aggctttccg aagattggca actgctgcgc
     1861 tggtcttcgc catctgcatc agggacaaca ttccttcatg catccttgcc ccaccaccac
     1921 cagcagagat gactacaaaa ggaatccgtt gctccaaact gcgctcggcg gcgagggtga
     1981 tctt
//
LOCUS       DAOQUC010000814         1984 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_73372_length_1984_cov_2.661483, whole genome shotgun sequence.
ACCESSION   DAOQUC010000814 DAOQUC010000000
VERSION     DAOQUC010000814.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1984)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1984)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1984
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73372_length_1984_cov_2.661483"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="PKH07_12940"
     CDS             <1..223
                     /locus_tag="PKH07_12940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35889.1"
                     /translation="VSMDIGAHEGGYWCVKASLNNRDWDTILEGRSSREWHEFDLTQY
                     AAAPVFLKITGDDFHQQQLGEFVLTAVAE"
     gene            complement(242..583)
                     /locus_tag="PKH07_12945"
     CDS             complement(242..583)
                     /locus_tag="PKH07_12945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011943147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HNT35890.1"
                     /translation="MKLIVAYIQPEKLNDVKRSLYKADVYKMSVTNALGCGQQRGYHE
                     SYRGVDVEVNLLKKVRLDIAVNEAFVEPTIEAIVQGARTGEIGDGKIFILDLLECVRI
                     RTGERGNEAIG"
     gene            complement(610..1938)
                     /locus_tag="PKH07_12950"
     CDS             complement(610..1938)
                     /locus_tag="PKH07_12950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014323831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HNT35891.1"
                     /translation="MALRHRFSVALVSSVLATRCYAQDTVPATPEVGLDTVWVLLAAF
                     LVFLMQAGFGMVEAGFTRAKNACNILTKNFMDYCIASIMFFLIGYAFMFGAGNAFIGM
                     NGFGLSDPSNPSGVPIFAFWLFQAAFCGAAATIVAGAMAERMKFPAYLIYTAIISGFV
                     YPIIGHWIWSSNGWLSAMGFGDFAGSTVVHAVGGCVSLVGTYMLGARIGKYTSDGTPR
                     AIAGHSIPLASLGVFILWFGWFGFNPGSTLSVGDGSTIALVAMNTNLSATAGALTAMA
                     TVWIMFGKPDLSMTMNGALAGLVAITAPCAFVSPGASICIGAIGGVIVVFGVLLLDRI
                     GIDDPVGAVPVHGMNGIWGTLSVGIWGQQALGLSRNGLLHGGGVWQLAIQAFGALSAC
                     LFALITMGIVFKVIQMTVGLRVSKEEELRGLDIGEHGMEAYAGFQVFTTQ"
BASE COUNT          514 a          548 c          480 g          442 t
ORIGIN      
        1 tgtttcaatg gacatcggtg cccacgaagg tggctactgg tgcgtcaagg cgtctttgaa
       61 caatcgggat tgggacacga ttctcgaagg acgtagttca agagaatggc atgagtttga
      121 tctgactcaa tatgctgccg ctcctgtctt tctcaagata accggcgacg atttccatca
      181 gcaacaactg ggggagtttg ttctgacagc agtcgcggag tgaattgctg ggaggcatct
      241 gtcagccgat cgcctcgttg cctcgctcgc ctgtgcgaat gcgaacgcat tccaggagat
      301 cgagaatgaa tatcttgccg tcccctattt ctccagttct tgccccctga acaattgctt
      361 caatcgttgg ttcaacaaac gcttcattca cagcaatatc gagtcttact ttcttcagaa
      421 gattgacttc gacgtctact cctctgtagg actcatgata gcctctctgc tgaccgcacc
      481 cgagtgcgtt tgttaccgac atcttataga catccgcctt atagaggctt ctcttgacgt
      541 cattgagctt ttccggctga atgtatgcca cgatcagttt catattctga tctcctttcc
      601 tctctgaatc tactgggttg tgaagacctg gaaccctgca taggcttcca tcccatgttc
      661 gccaatgtcc aaaccccgga gttcctcttc cttggagacc cgcagtccca cagtcatttg
      721 aatcaccttg aaaacgatcc ccattgtgat tagggcaaag agacaggcgc tcagtgcgcc
      781 gaatgcctga atcgccaact gccagactcc cccaccatgg agcaggccgt tgcggctcag
      841 tccaagtgcc tgctgacccc atataccaac tgaaagagtt ccccagatcc cgttcatccc
      901 gtgcacggga actgcaccga ctggatcatc aatgccaatg cggtcaagca gcaacacgcc
      961 aaagactacg ataacacctc caatggctcc aatgcagatg gaagcaccag gtgagacaaa
     1021 ggcacagggg gctgtgatgg ccacaagacc agcgagtgcc ccgttcatgg tcatcgagag
     1081 atcgggtttc ccaaacatga tccacacggt cgccatggcg gtcagcgcac ctgccgtagc
     1141 ggagaggttt gtgttcatgg caaccagggc gatggtcgaa ccatcaccga cactgagtgt
     1201 tgagcctggg ttgaatccaa accatccgaa ccagagaatg aagactccca gcgaggcaag
     1261 gggaattgaa tgtcccgcga ttgctcgagg cgttccgtca gaagtgtatt tcccaattcg
     1321 agccccaagc atatacgttc ccacaaggga aacgcatcca ccgaccgcat gaaccacagt
     1381 tgaacccgcg aaatcaccaa agcccattgc cgacagccag ccgttcgaac tccatatcca
     1441 gtggccaatg attggataga cgaacccgct tatgatagct gtgtaaatga ggtatgcagg
     1501 gaacttcata cgttccgcca ttgctcccgc cacaatcgtc gcagcagctc cacaaaaggc
     1561 tgcttggaag agccaaaatg caaatatcgg cacaccagat ggattgcttg gatcggatag
     1621 cccaaaacca ttcatcccta tgaaggcgtt acccgctcca aacataaagg cgtatccgat
     1681 caagaagaac atgatgctgg cgatacaata gtccatgaaa ttctttgtca ggatgttgca
     1741 ggcattcttt gcacgagtga atccagcctc caccattcca aagccggcct gcatcagaaa
     1801 gacaagaaat gcagccaaca aaacccagac tgtgtcaaga ccgacttcag gagtggcggg
     1861 gactgtatct tgagcatagc atcgtgtggc caagacagaa gaaacgagcg ctacggaaaa
     1921 tctgtgcctt agtgccatgc gaagaactgt ggttttggag aaccatgctc cagaagaatg
     1981 tgga
//
LOCUS       DAOQUC010000815         1982 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_73471_length_1982_cov_3.136482, whole genome shotgun sequence.
ACCESSION   DAOQUC010000815 DAOQUC010000000
VERSION     DAOQUC010000815.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1982)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1982)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1982
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73471_length_1982_cov_3.136482"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="PKH07_12955"
     CDS             <1..242
                     /locus_tag="PKH07_12955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35892.1"
                     /translation="RVESGATTVLPFGSPYRVSPVWSFSGNDRSVVHFDWKIVGSAGE
                     VCNSLVVNSSFPKAPEIVIREKSGKKVASGKFEYG"
     gene            complement(601..1329)
                     /locus_tag="PKH07_12960"
     CDS             complement(601..1329)
                     /locus_tag="PKH07_12960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35893.1"
                     /translation="MRIDAKGIHYRELNERIHVAIAQGETEFILDNVQGHRYIGTGLN
                     EAIRITINGVPGQDLAAFMNGTEIVVHSNVQDGAGNTMNKGKIVVHGDAGDVLGHAMR
                     GGRIFIRGNVGYRAGIHMKAFREHFPVVVVGGVAKEYLGEYMAGGLIVVLGLDRDSQP
                     LVGDWMGTGMHGGLIYLRGDVQSHQLGREVGSAEMTDEEWAILQDILQEYATDFTLKE
                     SFQRDEFRKFYPQSARPYGRLYAY"
     gene            complement(1326..>1982)
                     /locus_tag="PKH07_12965"
     CDS             complement(1326..>1982)
                     /locus_tag="PKH07_12965"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNT35894.1"
                     /translation="VQSIQSDQGNVISATLKSGIRIDCELVIIAIGVLPNIDLVKNTA
                     IKTDRGIVVDGTLQSTVEGIYAAGDVAQAFDLVEGGTRPIPILPVAYRQGWVAGLNMA
                     GRRRLYRGGVAMNAVDVCGLPTISVGITVPATNEYEILSSLESSVPRYRKIVLRDNRI
                     VGFILIGDIDRAGILTGLIRDKIDVTSFKESLLTDDFGLLHVPKDYRKHIVSGMGIEV
                     "
BASE COUNT          489 a          562 c          485 g          446 t
ORIGIN      
        1 ttcgtgttga gagtggcgca acgaccgtct taccgttcgg ctcaccctac agagtctccc
       61 ctgtttggag tttctctggg aacgacagga gcgttgtgca ctttgactgg aagatcgtcg
      121 gttccgcagg ggaagtatgc aatagcctcg tggtgaatag ctcatttccc aaagcacccg
      181 agattgtcat tcgagaaaag agtggcaaga aagtcgccag cggaaaattc gagtatggtt
      241 gagggggatc gtgcacgtac tcatggcgag taccccaaaa tcccagtgaa gaatactccg
      301 tttcagtcat ctgcaaaaag gcggggccat tcaggttcaa gacggagccc acgatcgtca
      361 ggataaaaga ctgacaagtc ccggcgaaga ggtcggacgg gatagaacgt gactcttggt
      421 cggtaaagta gcatgaaaat aagagagtgc tgcccttcgg caacgctcag gatgtgtttg
      481 cgagcgagcg cgttccagga tcttcttcgc aacaaagatt gtggaactca ctcatcacgg
      541 aagacaaagg gacgatatcc catagccttg tttgttttct cccagagata gggacggaga
      601 tcagtaggcg tacagccgtc cgtagggtct ggcactctgt gggtagaatt tgcggaattc
      661 atcccgttga aatgactctt tgagagtgaa atccgtagcg tactcctgga gaatgtcttg
      721 cagaatcgcc cactcctcat cagtcatctc ggcggagcca acctcacgcc ccagttgatg
      781 ggactgaaca tctcccctga ggtagatcag cccaccgtgc attcccgttc ccatccaatc
      841 tcctaccaga ggctgtgagt ctctgtccag tccgagaaca acgattagac caccggccat
      901 atactcacca agatattctt tggcaacacc cccaaccacg acaacgggaa agtgctcccg
      961 gaaagccttc atgtggatcc ccgccctgta gccaacattg cctcggatga atatcctgcc
     1021 ccctcgcatg gcatgaccca agacatcccc ggcatcacca tgaaccacta tcttgccttt
     1081 gttcatggta ttgccggcac catcctgcac attcgaatga acaacaatct ccgtaccgtt
     1141 catgaacgcc gccaagtcct gacccggcac gccgttgatg gtgattcgta tcgcttcgtt
     1201 caggccggtg ccgatgtatc ggtgcccctg aacgttgtcc agaatgaact cagtttcacc
     1261 ctgcgcaatc gccacgtgaa tgcgttcatt caactcacgg tagtggattc ccttggcgtc
     1321 aattctcata cttcaattcc catgcctgaa acaatgtgtt tgcgatagtc cttgggcaca
     1381 tgaagcagcc cgaaatcatc ggtcaacaat gactctttga acgaggtgac atctatcttg
     1441 tcgcgtatca atccggtcag gattccagca cggtcaatat ccccaatgag gatgaagccc
     1501 acgatgcggt tgtcacgaag cacgatcttt ctgtatcggg gcaccgaaga ctcgagcgaa
     1561 ctgaggattt catactcatt cgtcgctgga accgtaatcc caacggaaat cgtcggaagc
     1621 ccgcatacgt ccacagcatt catagccaca cccccgcggt acagccgacg tctccccgcc
     1681 atgttcaaac cggcaaccca accttgacga taggcaaccg gcagaatcgg aatgggtcga
     1741 gtgcctcctt ccacaagatc aaacgcctgg gcaacatcgc ccgccgcata gatgccttcg
     1801 acggtgctct gaagcgttcc gtccaccaca atccccctgt cagtcttgat cgcagtattc
     1861 ttcacaagat caatgttagg aagtactccg atggcaatga tgacaagctc acagtcaatg
     1921 cggatgccac tcttgagcgt tgctgagatg acattgccct gatcactctg gatggactga
     1981 ac
//
LOCUS       DAOQUC010000816         1981 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_73520_length_1981_cov_3.063344, whole genome shotgun sequence.
ACCESSION   DAOQUC010000816 DAOQUC010000000
VERSION     DAOQUC010000816.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1981)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1981)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1981
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73520_length_1981_cov_3.063344"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1686
                     /locus_tag="PKH07_12970"
     CDS             <1..1686
                     /locus_tag="PKH07_12970"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HNT35895.1"
                     /translation="DGVRQQHGARRHGCPNSPVYRDYCAQIVQVLAKEFGKDPNIIGW
                     QIDNELYFSTPRGCCCPVCHQKFQQAMKARFGTIEALNAAWGMDLWSQTYQSFDQLPI
                     PLSRTWHHPSLLTAWMVFQSDSYVDFCNHQADLLHKAVKQPVGTDMMPFSGVNYQHIH
                     EKLDLVQYNHYHGMENFWENAFWCDFIRPLKPAPFWNTETATCWGGATTANGYKEPGF
                     CRANSWLPLAMGGEANLYWLWRSHWSGQELMHGSVVTSAGRPMHIFDEVQEISRGFDA
                     VREFLNGTRPIQTGVALHFSGFAWWLFEFQPLVHGFHYAASLMRQVYHPMIEVQLRPD
                     VIDPASSLDGYRLLFSPFLPALDEAGLKDRLRAWIESGGVWVVGPMSDIRTMEATKFR
                     HAPFGVLEDWAGIRCDYDIPGDPRQFSLQWSDSTPSEGSIWYHGLKTVDAEVLATYTE
                     GPFEGLAAVTTKKLGKGRIIVLGTLPTPEDLKSVLLTIAQEAGVSPVADATGNLMVVP
                     REGKAGKGMILVEMLNQTASITLREPVTDLLTGKTHSGMVDVPPYGVLVLKKA"
     gene            complement(1714..>1981)
                     /locus_tag="PKH07_12975"
     CDS             complement(1714..>1981)
                     /locus_tag="PKH07_12975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008317631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="permease"
                     /protein_id="HNT35896.1"
                     /translation="LWTNLFASVFGAFMYFATLTEVPILQGLIGAGMGKGPALALLLA
                     GPALSLPSILVIRSIMGTKKTVVFCSLVVGMATISGMIFGAIWG"
BASE COUNT          438 a          545 c          564 g          434 t
ORIGIN      
        1 gatggagttc gccagcagca tggtgccaga cgtcatggct gcccgaacag ccctgtctat
       61 cgtgactact gcgcccagat cgtccaggtg ttggcgaaag agtttggcaa ggatcccaac
      121 attatcggct ggcaaattga caatgaactc tacttctcca ctccacgggg ttgctgctgt
      181 ccggtatgtc accaaaagtt tcagcaggcc atgaaagcgc gcttcggcac cattgaggcg
      241 ttgaatgcgg cgtggggaat ggatctgtgg agtcagacat atcagagttt tgaccagctg
      301 ccgatcccgt tgtcacgcac ctggcatcat ccttcgctgc tgaccgcttg gatggttttt
      361 cagtcggact cctatgttga tttctgcaac caccaggctg atctgttgca caaggctgtg
      421 aagcaacccg tggggacgga catgatgcct ttcagtggtg tcaactatca gcacatccac
      481 gagaaactcg atctggttca gtacaaccac tatcacggga tggagaactt ctgggagaac
      541 gccttctggt gtgacttcat cagacccttg aagccggcac ctttctggaa caccgagacg
      601 gcaacctgtt ggggcggtgc cacaacagcc aatggataca aagaacccgg tttctgccgc
      661 gccaactcat ggcttcccct tgcgatgggt ggcgaggcga atctctactg gctctggcgc
      721 tctcactgga gtggacagga actgatgcac ggctcagtgg tcaccagcgc cggacgcccg
      781 atgcacatct ttgacgaagt ccaggagatc tcccgcggct ttgatgcggt gcgggagttt
      841 ctcaatggca ccaggccaat acagactggt gtggcgttgc acttttccgg ctttgcctgg
      901 tggctgttcg agtttcagcc gttggttcat ggatttcact acgccgccag tctcatgcgt
      961 caggtctacc acccgatgat tgaggttcag ttgcgacctg atgtcattga tccggcgtca
     1021 tcgctcgatg gctatcggct gttgttctct ccatttctcc ccgcgttgga tgaggcaggc
     1081 ctgaaagacc gactccgcgc ctggatcgaa agcggtggcg tctgggtggt gggtccgatg
     1141 tccgacattc gaaccatgga ggccaccaag tttcgtcatg ctccattcgg agttctcgaa
     1201 gactgggcag ggattcgatg cgactacgac attcccggcg atccgaggca gttctcattg
     1261 cagtggtcgg attccactcc gtcggaaggc tccatctggt accatggact gaaaaccgtt
     1321 gatgccgaag tcctggcgac gtacacagaa ggcccgtttg aagggctcgc agccgtaaca
     1381 acaaagaaac ttggcaaggg acgcatcatc gtcctcggaa cacttccgac acccgaggat
     1441 ctgaagagcg ttctgttgac cattgctcag gaagcaggcg tgtctccagt ggcagacgcg
     1501 acgggaaacc ttatggttgt gccacgggaa ggcaaagcag ggaaaggcat gattctggtc
     1561 gaaatgctca accaaacagc gtctatcact cttcgggaac cggtgaccga tctcctgacc
     1621 ggcaagacgc actcgggaat ggtggatgtg ccgccgtacg gagtgctggt gctgaagaag
     1681 gcatgaaaat acagacttga ccgatcgcag ggactatccc cagatcgctc cgaagatcat
     1741 gccgctgatg gtggccatac cgacaaccag cgagcaaaat acgacagtct tcttcgtacc
     1801 cataatactt cgaatgacga gaatgctcgg gagcgacaat gcaggccccg ccaagagcag
     1861 tgccagtgcc ggcccctttc ccatgcccgc tccgatgagt ccctgaagga tgggtacctc
     1921 ggtcagtgtg gcgaaataca tgaacgcccc aaagacgctt gcgaagagat ttgtccaaag
     1981 g
//
LOCUS       DAOQUC010000817         1976 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_73820_length_1976_cov_3.112441, whole genome shotgun sequence.
ACCESSION   DAOQUC010000817 DAOQUC010000000
VERSION     DAOQUC010000817.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1976)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1976)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1976
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73820_length_1976_cov_3.112441"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1433
                     /locus_tag="PKH07_12980"
     CDS             <1..>1433
                     /locus_tag="PKH07_12980"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNT35897.1"
                     /translation="KVLTQAKIMVGGDIMLAKGESALARFPFLDAAYLNYTTGDVVEW
                     LCRAGDSAIPNIIFRSHNRILVGERREAKNFSCGIPQHELFPIKRYRFPFCRSARYSS
                     VITSFGCPLKCAFCVPGTFGLSLRPVDEVIEELRHLKSMGISEVLFQDSTINASREHS
                     RTLFSAIRESGLNIQWMCQSRADCVNDEILGEMRASGCHTIQFGVESGSDSIREGVNK
                     RCATAQYEESFALCHKHGIRPSGFFIIGLPGETHETVRETVELAKHLRCSTAVFGLAM
                     PHPVTALGALTGSQQSGLSENDHYDNYQRIGSQLSELTPEQVVRWRLKAYASFYLRAS
                     YVWQRLTEIGSLYELGDTVRNALHLIRENLAARGLKRPVGEPSEQISAQPLHRVSLLG
                     HNRRVAVIGGGVLGMTAAHRLASNGVSVSLIEKEGCLGGLSAPAEVRPGSWIDQHHHF
                     VAGTDSYLFGLLDELGLSKALSWSDTT"
     assembly_gap    1434..1443
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1444..>1976
                     /locus_tag="PKH07_12985"
     CDS             <1444..>1976
                     /locus_tag="PKH07_12985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35898.1"
                     /translation="PFTTAFDLLRFPGLNLAEKIRFVRSIRKLQQCGNWKELESISVE
                     DWYREQCGDRLYEVLWKHMMDCKFGEAAKEVPLSWMWARLTRRIATRRLGRGREHFGR
                     LVGGTRGFLDVLEDSIRAMGVTVRKGCSVKRLIVEDGRLVGLHTEAGTIECDAAIATI
                     PNPDLLSLWDGWQEGYA"
BASE COUNT          422 a          509 c          580 g          455 t
ORIGIN      
        1 ccaaggttct gacccaggcg aagatcatgg ttggtggcga tatcatgctt gccaaagggg
       61 aatcagcgct tgcgagattt ccttttctcg atgcggcgta tctgaactat acgacgggcg
      121 atgttgttga gtggctttgc agggcaggag acagcgctat ccccaatatc atcttccgat
      181 cgcacaatcg gattctggtt ggggaaagac gggaagcgaa gaatttctcc tgtggcattc
      241 cccagcatga actcttcccc atcaagaggt atcgctttcc cttctgcaga tccgctcggt
      301 actcttcggt catcacctca tttgggtgtc ccttgaaatg cgcattctgc gtccccggaa
      361 catttgggct gtccttgcga cctgtggatg aggtcatcga ggaacttcgg catcttaaga
      421 gtatgggaat ctcggaggtg ctgttccagg actcgacgat caatgccagc agagagcatt
      481 cacggacgct cttctcagcg atccgcgaaa gcggactcaa cattcagtgg atgtgccaat
      541 cccgtgccga ctgtgtcaac gatgaaatcc ttggtgagat gagagcctcg ggatgtcata
      601 cgatccagtt tggtgtcgaa agcggcagcg actcgatccg ggaaggggtg aacaagcgtt
      661 gtgccacggc gcagtatgaa gagagttttg cgctttgtca caagcacgga atcaggccat
      721 ccggcttctt tatcatcggg ttgcctggtg agacgcacga gaccgtcaga gagacggttg
      781 aactggccaa gcatcttcgg tgttccacag ccgtcttcgg cctggcaatg cctcatccgg
      841 taacggcttt gggtgccttg accggttccc agcagagtgg tctttccgag aacgatcact
      901 atgacaacta tcagcgcatc ggatcgcagc tgtctgaact gacacccgaa caggttgttc
      961 gatggcggct gaaagcctac gcttcattct atctccgagc atcctatgtc tggcagcgcc
     1021 tgacggagat cggcagtctc tacgaactgg gtgacacagt ccgaaacgcc ctgcacctga
     1081 ttcgagagaa ccttgcggca cgaggactga agcggccggt tggagagccg tcggagcaaa
     1141 tctctgctca gcccttgcac cgagtcagcc ttctcggaca caaccggaga gtcgccgtca
     1201 ttggaggtgg ggtgcttggg atgaccgccg cccatcgtct ggcgtccaat ggcgtcagcg
     1261 taagtctgat cgagaaagaa ggatgtctcg gaggcttgtc agcacccgct gaggtgaggc
     1321 ctggaagttg gattgaccaa caccatcact ttgttgccgg taccgacagt tatctgtttg
     1381 ggctgcttga cgaactgggc ctttctaagg ctctgagttg gagtgatacc accnnnnnnn
     1441 nnngccgttc acgacggctt ttgacctgtt gcgatttcct ggcctgaacc tcgctgagaa
     1501 gattcggttt gtgcgctcta tccgaaaact ccagcaatgc gggaactgga aagaactcga
     1561 atccatttca gtcgaggatt ggtatcgcga gcagtgcggt gaccgacttt atgaagttct
     1621 ttggaaacac atgatggact gcaagtttgg agaggccgcc aaggaggtgc ccctgtcctg
     1681 gatgtgggca cgactgacac gccgcattgc gacacggcgt ctgggcaggg gacgtgagca
     1741 tttcggtcgt ctcgtcggcg gaacgagagg attcctggac gtgttggaag actcaatacg
     1801 cgccatggga gttaccgttc gcaaaggctg ctcagtgaaa cggcttattg tggaagatgg
     1861 acgtttggtc ggtcttcaca ccgaagcggg aacaatcgaa tgtgatgctg ccatcgcgac
     1921 aattcccaac cccgatctcc tcagtctctg ggacggatgg caggagggtt acgcag
//
LOCUS       DAOQUC010000818         1976 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_73833_length_1976_cov_2.821447, whole genome shotgun sequence.
ACCESSION   DAOQUC010000818 DAOQUC010000000
VERSION     DAOQUC010000818.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1976)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1976)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1976
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73833_length_1976_cov_2.821447"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            352..1914
                     /locus_tag="PKH07_12990"
     CDS             352..1914
                     /locus_tag="PKH07_12990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35899.1"
                     /translation="MRTTMRRALILMTIAFAFVSGANAATVPVSANISTSVTWTADNV
                     YQLKQQIYVLPGATLTIEAGTLVQSLVADQGSLAVCRGAKIYVNGTVNNPVIMTSSND
                     TLTAWHEGCNEWGNLTIMGNGLVSASHYGGSPVVVNNEGTIRANTSVPDGLNEKQMEG
                     LTAGYPGDSKVLYGGNDDNDDSGSISYLSLRYGGKVIGLANELNGLSLGGIGRGTDIS
                     HVEIMNNVDDGIEIWGGTVNLKYVSIWNIGDDSFDVDEGWRGKVQFGLIVQGHSTDAS
                     QGSGVGDNAFEMDGAEDSDAQPVTTACIYNFTTIGQPVHGDHGTAWRDNARVQFRNCI
                     FMDLGERLVQNDNSDGDGGNGYGYNGTLSWANTWTTDYSVTSPVNIGSASPALLYQAQ
                     TSGKLAEIKDSVFYNNLFATAYTEADARGVRDAGNNNVTATLMPIQSITRGTPVTKGG
                     RTMVPVTHVIPCAANDATSSVAQAPSDGFFTPAGYRGGFSSTDNWLVGWTAAHAYGMT
                     SSPSCVLNSEMY"
BASE COUNT          504 a          475 c          542 g          455 t
ORIGIN      
        1 agctcatctg ggagagcgcc gcccttgcaa ggcggaggtt gccggttcga tcccggtcgt
       61 ctccaccaaa tccaaataaa cctcacagta tccaatcggt ttcctttctc aattcatacg
      121 tgcttcgcat cgtgtcctaa caccactgat tcatcttcta cagcatacga atgacctgtg
      181 tgatcagact cttgtcgcca atagtccatg gatcacggaa aggagaagaa tcaacggata
      241 acacgagcgt tggcatatcc cagaaagaat ccaagacaga atctttgtgg gagtctctca
      301 agtgctcccg tcagggagct ccaaattcga ttaagaaagg atagacaaaa aatgagaaca
      361 acaatgagaa gagccttaat tctgatgacg attgcttttg ccttcgtgtc tggtgcgaat
      421 gccgcaacag ttcctgtttc ggcaaatatc agcacgtcag taacatggac agcagacaac
      481 gtctatcagt tgaaacagca gatctatgtt ttgcctggcg ccacactgac gattgaagca
      541 ggaacactcg ttcagagttt ggttgccgac caaggaagtc tggcagtctg ccgaggagcg
      601 aagatttatg tcaatggtac ggtgaacaat ccggtcatca tgacttcttc caatgacacc
      661 ctaaccgcgt ggcatgaagg ctgtaacgaa tggggaaatc tgaccatcat gggcaatggc
      721 ctggtttcgg cgtcgcacta tggtggcagt cctgttgtgg tgaacaacga aggaaccatc
      781 cgagctaata cttctgttcc tgatgggctc aatgagaagc agatggaagg tttgacggcg
      841 gggtatcccg gtgactccaa ggtgctgtat ggcggcaatg acgacaatga tgacagcggc
      901 tcgattagct atctgtcgct gcgatatggc ggcaaagtca tcggtcttgc caacgaactc
      961 aacggtcttt cgctgggtgg cattggtcgt ggaacggaca tcagccatgt agagatcatg
     1021 aataatgtgg atgatggcat tgagatctgg ggtggaacag tcaacctgaa gtacgtcagc
     1081 atctggaata ttggcgatga tagttttgat gttgatgaag gatggcgtgg caaagtgcag
     1141 tttggactga ttgttcaagg ccatagtacc gacgccagcc aaggttcagg cgttggtgac
     1201 aacgcctttg agatggacgg cgcagaggac tccgatgcac agcctgtgac aaccgcctgc
     1261 atttacaact tcacgacaat agggcagcct gttcacggtg atcatggaac agcctggcgt
     1321 gacaatgctc gtgtgcaatt caggaattgc atcttcatgg atttgggtga acgtctggtc
     1381 caaaatgaca actcagatgg agatggcgga aacggctatg gctacaatgg aacgctgagc
     1441 tgggcgaata cctggacgac ggattactcg gtcacctcac cggtgaatat cggttccgcg
     1501 agtcctgctc ttctgtatca ggcgcaaaca tcaggcaagt tggccgagat aaaggacagc
     1561 gtcttttaca acaacctttt tgccaccgcc tacactgaag cggatgcccg aggcgttcgg
     1621 gatgccggca acaacaatgt tacggcgact ttgatgccga ttcagtccat tacgcgcggg
     1681 accccagtca ccaagggtgg cagaacaatg gttcccgtaa cacacgtaat accgtgtgcg
     1741 gcaaatgatg caacaagcag tgttgctcaa gccccgagcg acggtttctt cacccctgct
     1801 ggctatcgcg gcgggttcag ttcaacggac aactggcttg ttggctggac ggcagctcat
     1861 gcctatggga tgacatcatc acccagctgc gtgctgaact cagagatgta ctaggacaga
     1921 atgagctata agtgcaatgt caggctgagg cgggagttgt tcccgcctcg gcctgt
//
LOCUS       DAOQUC010000819         1972 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_74060_length_1972_cov_2.723005, whole genome shotgun sequence.
ACCESSION   DAOQUC010000819 DAOQUC010000000
VERSION     DAOQUC010000819.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1972
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74060_length_1972_cov_2.723005"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1972
                     /locus_tag="PKH07_12995"
     CDS             <1..>1972
                     /locus_tag="PKH07_12995"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Members of the Solute:Sodium Symporter (SSS), TC
                     2.A.21 as described in tcdb.org, catalyze solute:Na+
                     symport. Known solutes for members of the family include
                     sugars, amino acids, nucleosides, inositols, vitamins,
                     urea or anions, depending on the system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sodium/solute symporter"
                     /protein_id="HNT35900.1"
                     /translation="GKKVWRDDVYVLEKTSTGQYQWHIGFRLPRALAHGASVSTERGV
                     VCIGGCDAERCYSDVFVIRWDSVSKTIDFETLPALPQPCAFGAAVVSGKTVFVAGGQS
                     TMLEAFAMRQFWSLDLTDGSQWQDLPQWNGSPRILPVLAAQNFQDSRNIYLFSGRDVA
                     PNRDTILLKDVHRYDPATQIWTQLSDAPRCLMAGMGVGFGAQHILLVGGDDGENWGKD
                     LRDTHPGFAKDMVFAYHTLTDTWVQHGTLPQNHVTTSLIRWGDAFIIPTGEIRPAVRS
                     DIIWKATPEKSQQNCQPLDYIVIVLYLLALIGVGVYCAKREKTTDDFFLAGRRIPWWA
                     AGLSIFGTQLSAITFMAIPAKTYSSNWLTFLFNMGIVAITPLIVFFFLPFYRRLNVTT
                     AYEYLERRFSVTVRVLGSALFLAFQLGRIGIVLLLPSLALSVVTGVNVYFCILIMGVL
                     ATVYTVMGGIEAVIWTDVFQVFVLVGGAVWCLLYATAHVSGGISGVFATALAENKFHW
                     VDWNWDLGRLTIWVIVLGWINSLLPYASDQTVIQRYLTTKDEKAARQAIWTNAILTVP
                     ASLLFFSLGTALFVFYKNHPNHLDPGMAKVDNILPWFVMRELPAGVSGLVIAAVFAAS
                     MSTLDSSMNSMATAVVTDFCRRFRRNSDEAYCL"
BASE COUNT          384 a          501 c          565 g          522 t
ORIGIN      
        1 gtggaaagaa ggtttggcga gacgatgtct acgttctcga aaagacttca accggacagt
       61 atcaatggca catcggcttc agattgccca gagccttggc acacggagcc tcagtctcaa
      121 cagaacgtgg tgtcgtgtgc atcggtggat gcgatgctga aagatgctac tccgatgtct
      181 ttgtcatccg atgggattct gtttcaaaga ccattgactt cgagacgctt ccggcactgc
      241 cgcagccctg tgcatttggc gcggcagtcg tctcggggaa gacggtcttt gttgccggtg
      301 ggcagtccac gatgctggaa gcgttcgcga tgcgacagtt ctggtctctc gatctgactg
      361 atggttccca atggcaggac ttgcctcagt ggaacggctc accacgaata cttcccgttc
      421 tcgccgcgca gaatttccag gattctcgga atatctacct tttcagtggt cgcgatgttg
      481 caccgaacag ggacaccatt ctcctgaagg atgttcaccg gtacgatcca gcgactcaaa
      541 tatggacgca gctgtccgat gcacctcgat gtctgatggc tggtatgggg gtgggcttcg
      601 gtgcccaaca catcttgctt gtgggtggag acgacgggga gaattgggga aaggacctgc
      661 gcgacacgca tcctgggttt gccaaagata tggtgtttgc ctatcacacg ctgacggata
      721 cttgggtgca gcatgggaca cttccgcaga atcatgtcac cacttcgttg attaggtggg
      781 gcgacgcctt catcatccca accggtgaga taaggcctgc tgttcgttct gacattatct
      841 ggaaagccac acctgagaag tcccaacaga actgtcagcc cttggattat atcgtcattg
      901 ttctctatct cctggcgctc attggtgttg gtgtctactg cgccaagcgg gagaagacaa
      961 ccgacgactt ctttctcgcc ggacggcgga ttccctggtg ggctgccggt ctgagcattt
     1021 tcggcacaca gttgagcgcc ataacattca tggcgatacc agccaagaca tattcgagca
     1081 actggctgac gtttctcttc aatatgggca ttgtcgcaat cacgccgctg atcgttttct
     1141 tcttcctgcc attctatcgg cgtctgaatg tgacgacggc ctatgaatac cttgagaggc
     1201 ggttcagcgt gaccgtgcgg gttcttggca gtgctttgtt tctcgccttc caactcggga
     1261 gaatcgggat cgttctcttg ctgccgtcgc tggctctttc ggtggtgacg ggcgtgaatg
     1321 tctatttctg cattctgata atgggagtgc tggccactgt ctatacggtc atgggcggca
     1381 ttgaagcagt gatatggact gacgtttttc aggtgtttgt gctggttggc ggtgcggtct
     1441 ggtgtcttct ctatgcgacg gctcatgtca gtggagggat ctcaggtgtc tttgccacag
     1501 cgcttgcaga gaacaagttc cattgggtgg attggaactg ggatcttgga cggctgacaa
     1561 tttgggtcat cgttctggga tggatcaaca gcctgcttcc ttacgcctcg gatcagacgg
     1621 tcatccagcg gtatctgaca accaaagatg agaaggcggc gcgtcaggcg atttggacaa
     1681 atgccatcct caccgttccc gcgtctcttc tgtttttctc tctcgggacg gcgctctttg
     1741 tgttctacaa gaaccatccg aatcatctgg atcctggcat ggcgaaggtt gacaacattc
     1801 tcccctggtt tgtgatgcgg gaattgcctg ccggagtgag tgggcttgtc attgctgcgg
     1861 tgtttgccgc ttccatgtcc actctggaca gcagcatgaa cagcatggcg actgcggtgg
     1921 tcacggattt ctgccgacga tttcgcagaa actctgatga agcgtactgc ct
//
LOCUS       DAOQUC010000820         1969 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_74200_length_1969_cov_3.025601, whole genome shotgun sequence.
ACCESSION   DAOQUC010000820 DAOQUC010000000
VERSION     DAOQUC010000820.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1969)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1969)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1969
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74200_length_1969_cov_3.025601"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(43..852)
                     /locus_tag="PKH07_13000"
     CDS             complement(43..852)
                     /locus_tag="PKH07_13000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35901.1"
                     /translation="MVRSRKDMRTTDFGVLYFVDDNPGYQRMLRASIESLRRFHPDWP
                     VKVIAQRCYHVPLWKRIYRGVSVWKWSARHARSNMDYRVICAKNEAFCQTPFELTLFI
                     DADTVIMRPLDTLRRQAEGHDVLANPFPWCQYQGLEPWQPTTFQHLNSGVIFYNRRFI
                     DVFREYSERTKHTLGRYHANQFTFSLACEMEKERLRIGLDSSIQVETMNLAKILQTGG
                     YPRVGDLIDLRWEGLSRFHVFHYNGPHKAEYLALIKQLWNLPSDEKGLDAG"
BASE COUNT          458 a          542 c          503 g          466 t
ORIGIN      
        1 cacgaacgcc atcggggcag ccacatgcag cacccagaat gcttaccctg catccaatcc
       61 cttttcatcg gatggtaggt tccagagttg cttgatcaat gccaaatact cagccttatg
      121 gggcccatta tagtgaaaca catggaaccg tgataagcct tcccatcgga ggtcaattaa
      181 gtcaccaact cttgggtagc cccccgtctg gaggatcttc gccaagttca tggtctccac
      241 ttgaattgaa gagtctagcc cgatccgaag tctttccttc tccatttcgc aggcaaggct
      301 gaaggtgaat tgattcgcat ggtaacggcc aagggtgtgt tttgttcgtt cggaatactc
      361 gcggaataca tcgatgaatc gtctattata gaagatcacg cccgagttca ggtgttgaaa
      421 cgttgtgggc tgccatggct caagaccctg gtactggcac catggaaacg gattcgcgag
      481 aacatcatgc ccttcagcct gtcttcgcag tgtgtccagt ggccgcatga tgacggtgtc
      541 ggcatcaatg aaaagcgtca actcaaatgg cgtttgacag aacgcctcgt tcttggcaca
      601 gattacgcga tagtccatgt tcgaccgcgc gtggcgagcc gaccatttcc aaacagacac
      661 tccgcggtag atcctcttcc ataaagggac atggtagcag cgttgggcga tcaccttcac
      721 cggccagtcc ggatggaatc gcctcagcga ctcgatactc gcccgcagca ttctctggta
      781 gcctggattg tcgtcaacga agtacagcac cccgaaatcc gtggttctca tatccttccg
      841 ggatctaacc atatgcttct cctgcatcgt agatcatgcc ctccgcaacc cccacagctc
      901 gcgcagtcgc gtgctgcacc tgcgacgcaa ccggcgggcc caacgacgtg cgttcctgta
      961 tccctgcgcc ggcaacgtgg gggggatcat ctgcattgtg tctgtgcaga acacgtattt
     1021 tccagataga tgcgcgattt ccattgcctg ctgtggcgaa ctctctatga atagacatgt
     1081 ctgtgtgtca cgataatgcc ttgctttgaa gctcgcgtgg gagccggaag ccaagcgggc
     1141 ctgtttatct ggtagatcca tcatgagaag cgtgcggaat tgaatgccat tgcgcacgag
     1201 ccattcttca gtgtctcggc ggtatttctc gagccgtgaa gtgaccagcc atcctactgg
     1261 gactgttggc acgaacgtcg ggcggacttc ccttatgaac tggagatatt gtgggccatc
     1321 atcattctct tccggtgtgg gatcacggca gaggacccca tcgatatcca cgcacgacat
     1381 agccaacaca ccatgatgta gaatattcca ttcaaaataa cgaggcgtag gaactatggt
     1441 gtagtacgag tccacaagtt ggtcggcatc tcgcgaagca gcatagactg ctccgtagag
     1501 tatgacatga ggaaggcttg ccgatgttat gagttgccta acaagctgct tctgctgtcc
     1561 tgccgatacg gtatcgtcca caatcagaac cttgcgaggg acagagagaa atgttggccc
     1621 atcaatccta ccgcttgtgc gctgtccaca cgcaaacact cttccgcaaa ccagcccttc
     1681 tacgtctgtc atgggaaggt tcagatgaag agccagaaga ttggctgcga gcaacccgct
     1741 ccgtggaatc cccacaatca actccaaatc tctgggtaat aacttggccc aagcgataat
     1801 atcgcgattg agatcggcca cactgcggta attcatggtt tggtgcctgt cattccaaga
     1861 ttgatccagc cgtggtcgta tccgcgaacc tcttcatgcg tcaattctct gacgccagaa
     1921 agcccaacgg tctcgaatag tcgccgcaga taccttggtg taaggaggg
//
LOCUS       DAOQUC010000821         1969 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_74205_length_1969_cov_2.896552, whole genome shotgun sequence.
ACCESSION   DAOQUC010000821 DAOQUC010000000
VERSION     DAOQUC010000821.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1969)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1969)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1969
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74205_length_1969_cov_2.896552"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1969)
                     /locus_tag="PKH07_13005"
     CDS             complement(<1..>1969)
                     /locus_tag="PKH07_13005"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNT35902.1"
                     /translation="EVPTLYSYINSLSFRVFGINVFAARATGLAFATLTIAAMYFFCR
                     YVFGVPLGLLATALFAASRYHITMSRWGASEIAPSFYMLMSFGLLLRAIQVREGMTPP
                     VKQGTGFWKSPDQLCTLGASLLSVALMSFLLSRVTLGFREPQILVRLSEYHARIQAWQ
                     GFKVIALLCPLMALGLFLRALLTCKYRADRLTPGDFLRWPGWLFIASGLFLGLCIYTY
                     LASRLVAVVVCAYMIALAVLRFRLFRRHFWGLFLLGMVFCMVFSPLAYHYGSHWLVFT
                     HRWHDINIFKFCREAGNMRPFWVSLQRHLLMFNWMSNEVIRHGMPGKPLLNGISGVLF
                     ALGAAFALRRVRHPFYCFVHLWFWLTLMGGILSFRGAHESPCSYPVLTCAPAIYVFII
                     IAALEFWDGLVVFFSGVRFRLFKGVFWGVLLLLSTLASILDLYVYFGIQAKDPRMWAH
                     YNPVEHEAAVRVKKYTEDHHVFLNPHYFTFSTVELLNYPYRNVEKFLGVEALPFVMPE
                     DKPVAMIMSYEDAWLEPMLRKFYPNVRMVPYEDPVTKGIGFKEFFVPKQDIENSRGLI
                     ATYTLDSDQGVSKRRRETSLDVTISAATLDLIQDREGTGLSVLWEGLIQIPVLGEYWF
                     QLQMEPALDHADVEIEVSGSKVVEQGRARPVT"
BASE COUNT          589 a          544 c          478 g          358 t
ORIGIN      
        1 cgtcaccggt cttgcccttc cctgctcgac aactttgcta ccagaaacct ctatctcgac
       61 atctgcatgg tcgagtgccg gctccatctg caactgaaac cagtactccc ccagcactgg
      121 aatctgaata agcccttccc agaggaccga aagcccagtc ccctctctgt cctggatcag
      181 atccagagtg gctgctgaga ttgtcacgtc cagagaagtc tcacgccgtc gcttggaaac
      241 accttgatcg gaatccagcg tgtaggttgc tatcagtcct ctgctgttct cgatatcctg
      301 cttcggaaca aagaactcct taaacccaat tcccttcgtc actggatctt catagggaac
      361 catccgaaca ttcggataga attttcgaag catcggctcc aaccaggcat cttcatagga
      421 catgatcatg gcaaccggtt tgtcctctgg catgacaaaa ggaagagcct cgacacccaa
      481 aaatttctcc acgtttcgat agggatagtt caacagctcg accgttgaaa atgtaaagta
      541 gtgaggattg agaaagacat gatgatcctc agtatatttc ttgacacgaa cggccgcctc
      601 atgttcgacc gggttgtagt gtgcccacat tcggggatcc ttggcttgaa tcccgaaata
      661 cacatacaaa tccaggatac tcgccaaagt gctcaacaga agaagcacgc cccagaatac
      721 gcctttgaac aagcgaaacc tcacgcccga aaagaacaca accaagccat cccaaaactc
      781 cagcgctgca atgatgatga agacatagat tgccggtgca caggtcagaa ccggatagga
      841 acacggcgac tcatgagcac cgcggaagga taggatgcct cccatcaggg tcagccagaa
      901 ccagagatgg acgaaacagt agaacggatg gcgaactcgc ctcagcgcaa aagcggcacc
      961 gagagcaaaa agcacgccgc ttatgccatt cagcaggggc tttcctggca tcccgtgacg
     1021 aatcacctcg ttgctcatcc agttgaacat cagcagatga cgctgaaggc tgacccagaa
     1081 cggcctcatg ttgcccgctt cacgacagaa cttgaagatg ttgatatcgt gccagcggtg
     1141 tgtgaaaaca agccagtgac ttccatagtg ataggccaaa ggtgaaaaca ccatgcagaa
     1201 aaccataccc aggagaaaga gaccccaaaa gtggcgtcga aagagtcgaa aacgcaacac
     1261 cgcaagggcg atcatgtacg cgcacacgac aaccgccaca agtcttgatg caagatacgt
     1321 atagatgcac agtcccaaaa aaagtcccga agcaatgaag agccagccag gccatctgag
     1381 aaagtcaccc ggagtcaacc gatccgcacg gtatttgcat gtaagaagag ctcgaagaaa
     1441 aagccccaag gccatcaagg gacaaagcaa ggcaatgacc ttgaagcctt gccacgcctg
     1501 aatcctagcg tgatactccg acaacctgac aagaatctga ggctctcgaa agccaagagt
     1561 cacgcgggag agcaggaagg acatgagagc gacactcaag agtgaagcgc ctagagtaca
     1621 gagttgatcc ggagacttcc agaaacccgt tccctgcttg accggtggcg tcatgccttc
     1681 ccgaacctgg atggctcgaa ggagcagtcc aaaggacatc agcatataga aagaaggcgc
     1741 aatctcggac gccccccatc ggctcatggt gatgtgatat cgagaggccg cgaaaagcgc
     1801 cgtcgccaag agtcccaatg gcactccaaa cacatagcgg cagaagaaat acatcgctgc
     1861 aatggtcaga gtggcaaagg caagaccagt agccctggca gcaaacacgt tgatcccaaa
     1921 cactcgaaat gacaatgagt tgatatacga gtacagcgtt ggaacttca
//
LOCUS       DAOQUC010000822         1967 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_74357_length_1967_cov_2.434623, whole genome shotgun sequence.
ACCESSION   DAOQUC010000822 DAOQUC010000000
VERSION     DAOQUC010000822.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1967
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74357_length_1967_cov_2.434623"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..365
                     /locus_tag="PKH07_13010"
     CDS             <1..365
                     /locus_tag="PKH07_13010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004623015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HNT35903.1"
                     /translation="WPEILSAFRTLGMDDPKDFYDAIITAGFPLRKGEVGTLGELSPK
                     PHPWLYAETCRVGLGIEFADRNSVVGIEDSGAGVCSIRLAGFPTVGFGGGNIIESGTR
                     ELCTHFCEDFAEVLEAIK"
     gene            430..1719
                     /locus_tag="PKH07_13015"
     CDS             430..1719
                     /locus_tag="PKH07_13015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35904.1"
                     /translation="MSLQNLSTTLTIALIVSGIWALRAEAQAQSEWKIGMPLVTYWAG
                     PGDTMSLDDRTAAQIRAGGWNLGWALHEEHLDILHRHGLRAMLQIGTPNVDDPAQVQT
                     LDALIERVKNHPALYSYHLVDEPGAGAFPALGKLVAHLRSKDPAHLAYINLFPTYANE
                     AQLQVSADAAERAKVGIPQDFAGVSTSDDTVLRYREHLRQFVEIVKPDLISYDHYHFL
                     KDSDGKQYFLNLALIRAAALEANKPFLNIIQACDSPSEGWRGPGENEVRWLTYTSLAY
                     GAQGICHFRYDVGLWEDRKNPSTPLPLYWAVSRMNREFLAIASELQPLKSIGAYHCGE
                     VPVGGNALPAGSVFGLQTSPQNVLLGYFGKSPGRPTHVVVVNLDYTNAISTTLTGPGA
                     MEIFHAMTQTWKSHSEDRHLKLDIEPDGGVLVRLNGQ"
     gene            1788..>1967
                     /locus_tag="PKH07_13020"
     CDS             1788..>1967
                     /locus_tag="PKH07_13020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35905.1"
                     /translation="MKKSEKTKPKVKKGTERVQCHFISNTHWDREWRYSSQRTRHMLV
                     YMLDMLFDLFEKSPDF"
BASE COUNT          473 a          526 c          522 g          446 t
ORIGIN      
        1 catggccaga gattctctct gcattccgca cactggggat ggacgaccca aaggatttct
       61 acgatgccat catcaccgcc ggtttcccgc ttcgtaaggg agaggtcggg acgctcggag
      121 aactttctcc caagccgcat ccgtggctgt acgcggaaac atgtcgtgtc ggactcggaa
      181 tcgagtttgc agacaggaat tccgtcgtcg gcatcgaaga cagcggtgca ggagtctgct
      241 ccataagact tgccggtttt ccaacagtcg gttttggtgg cggcaacatc atcgaaagtg
      301 gaacaaggga actctgcaca cacttctgcg aggacttcgc agaggtactg gaggctatta
      361 aataggacca agcattcgcg cgcgaatccg atatgtctta gcagcacagt ggatcatgga
      421 ggttcaataa tgtcacttca aaacctgtct acgacactca ccatcgcctt gattgtcagt
      481 ggcatctggg cactccgcgc tgaggcacaa gcgcagtcag aatggaagat tgggatgccc
      541 cttgtcacct attgggccgg acctggtgac acgatgtcac tggatgatcg aaccgcagcg
      601 cagattcgcg ccggtggttg gaaccttggc tgggcgctcc acgaagagca tcttgacatt
      661 cttcatcgcc atggacttcg agccatgctg cagattggca cccccaacgt agatgatcca
      721 gcacaggttc agactcttga tgcgcttatc gaacgggtga agaatcatcc ggctctctac
      781 tcctatcatc ttgttgatga accgggagct ggagcgtttc ctgctttggg caagctggtc
      841 gcgcacctgc gcagcaagga tccggcacat ctggcttaca tcaatctgtt ccccacttac
      901 gcaaacgaag cgcaactgca agtcagtgcc gacgctgctg agcgcgcaaa ggtcggcatt
      961 ccgcaggatt tcgctggcgt ctcaacgagc gacgacaccg tgctcagata ccgggagcat
     1021 ctcagacagt ttgtggagat cgtcaaaccc gatctgatca gttacgatca ctatcatttt
     1081 ctgaaagaca gcgatggcaa gcagtacttc ctgaacctgg cactgattcg cgcagcggct
     1141 ctggaggcga acaagccctt tctgaacatt atccaggcat gcgattctcc ttccgaaggc
     1201 tggagagggc ctggcgaaaa tgaagtgcgt tggctgacgt acacttccct tgcctatgga
     1261 gcacagggga tctgccattt tcgttacgat gtgggtctct gggaagaccg caaaaacccc
     1321 tcaactcctc tgccactcta ctgggcggta tcaagaatga atcgagagtt tctggcgatc
     1381 gcttctgaac tacagcctct gaaatccata ggcgcatatc attgcggcga ggttcccgtg
     1441 ggtggaaatg ccctgccggc aggaagtgtc tttgggctcc aaacgagtcc tcaaaacgtc
     1501 ttgctcggtt atttcgggaa atcccccggt cgccccactc atgttgttgt ggtgaatctt
     1561 gactacacga atgcgatctc aacgacacta acgggtcctg gcgcgatgga gatattccac
     1621 gcaatgaccc agacttggaa aagccactca gaagaccggc acctgaaact ggatattgag
     1681 cctgacggtg gagtgcttgt gcgtttgaac gggcagtaaa ctattcatct acggagaggc
     1741 gtatcgcctc atcctatgaa ccgcttgaat acaggagatc atgacagatg aagaagtccg
     1801 aaaagacaaa acccaaagtc aaaaagggca cagaaagagt tcagtgccac tttatctcta
     1861 atacgcactg ggatcgcgaa tggcggtatt cctcgcaacg cacccgccat atgctggtgt
     1921 atatgcttga catgcttttc gatctctttg agaaaagccc cgatttc
//
LOCUS       DAOQUC010000823         1966 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_74429_length_1966_cov_1.961800, whole genome shotgun sequence.
ACCESSION   DAOQUC010000823 DAOQUC010000000
VERSION     DAOQUC010000823.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1966
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74429_length_1966_cov_1.961800"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="PKH07_13025"
     CDS             <1..252
                     /locus_tag="PKH07_13025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35906.1"
                     /translation="KRLLEVQQYQDIRIFGRDNIQRTISEHLDGLENCEKEHLIKTLL
                     DRYERFLHPRMRQTKPAMLVRNAEEILISMAGVRGTLER"
     gene            complement(294..1694)
                     /locus_tag="PKH07_13030"
     CDS             complement(294..1694)
                     /locus_tag="PKH07_13030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35907.1"
                     /translation="MLGEELSMWEVLLDEWDQATSHLANAECFKRAGELKLSVPVPSH
                     LVYREQILSQKGEAAVRALADMDAKIGSATGKVKAGSDNGSVGLLSEGAAILWDLLEK
                     IRKEEALWPKPMFARAESQFARSVTAIRQCFGTWLKRQTPRDESPSAVGEFKMSMCQI
                     AGRLTKIGLTDLSSRLQEHTESLVRNVEIAAKATQLIRDVRNWITQSVSILRFVRIAE
                     IRSKVAAGHEYESKLEEMSRRVENPDIVSVQLELSKALTDMRSAESEVLKRAGKVWES
                     RVQSEDDIVSLLDEIVGLISAFEGCSNDLEDFRLLQRTLRMYQDMYRQLADDSLTWKE
                     LKDRSQLLMQRAEEEVGESEVPWLPKETLGSLLDSISKRRKQASQEWIGEIEAETTRV
                     SKMSASEAGRLHERASNSPAFLNDQDSKRLKKAVSTIERRLSTLKIEWMVEKFRELSA
                     AERGEFLSRIKKSPDK"
BASE COUNT          505 a          560 c          403 g          498 t
ORIGIN      
        1 aagagacttt tagaagtcca acaataccaa gacattcgaa ttttcgggag ggacaacatc
       61 caaagaacaa tcagcgaaca cctcgacgga ctcgaaaact gcgagaaaga acacctcatc
      121 aaaaccctat tggatagata tgaacggttc ctgcaccccc gcatgcgaca aaccaaaccg
      181 gcgatgctcg tcagaaacgc agaggagata ctgatatcca tggcaggcgt aagaggaaca
      241 ttggaaaggt gaacgtcttg gagtcccttc cgatgccccg atgtaaagac tctctatttg
      301 tcaggacttt tcttgatacg tgacagaaac tcgcctcgct cagccgccga caattccctg
      361 aacttctcca ccatccactc aatcttcaaa gtactaagtc ttcgctcaat cgtgctcaca
      421 gccttcttga ggcgcttact gtcttgatcg tttagaaatg cgggggaatt gctggcacgc
      481 tcatggagcc ttccagcttc agacgcagac atctttgaaa ctcttgttgt ctcagcttcg
      541 atctcaccga tccattcctg actggcctgc tttcgccgct tcgaaattga atccaagagg
      601 cttccaagcg tttccttggg aagccaagga acttctgatt ctccgacctc ctcctcagct
      661 ctctgcatca acaactgact ccggtcctta agctctttcc aagtcaggct atcatccgcc
      721 aactgcctat acatatcctg atacatgcga agagttctct gcaggagccg aaagtcctcc
      781 aaatcattgg aacacccttc aaacgctgaa ataagaccca caatctcatc aagaagagag
      841 actatgtcat cctcactttg aaccctagat tcccatacct tccccgcacg cttaagaacc
      901 tcgctttcag cagatctcat gtctgtaagt gccttggaga gttccagttg aactgagaca
      961 atgtcaggat tctcaacacg tctagacatc tcttctaact ttgactcgta ttcatgtcct
     1021 gccgcaacct ttgatcggat ttcagcgatt cgcacgaatc taagtatgga cacagactga
     1081 gtaatccagt ttcgcacatc acgtatgagc tgtgtagcct tagcggcgat ttcgacgttg
     1141 cgaacaagac tttccgtatg ctcttgaagc ctgctggaaa gatctgttag cccgattttc
     1201 gtcagacgcc ctgcaatctg acacatggac attttgaact ctccaaccgc gcttggggat
     1261 tcgtctcttg gagtttgcct cttgagccac gtgccaaaac actgcctgat ggcagtaact
     1321 gagcgtgcga attgcgactc ggctctggca aacatgggct tgggccacaa tgcctcttct
     1381 ttcctaatct tctcaagtag atcccacagg attgctgcac cttctgacaa taatccgact
     1441 gaaccattgt ctgaacctgc tttaactttc cctgtggcgc tcccaatctt tgcatccatg
     1501 tcggctagcg cacgtacggc tgcctcccct ttctgtgaaa ggatctgctc tctatagaca
     1561 aggtgggaag gcacaggcac agacaacttc aactctcctg ccctcttgaa acattccgca
     1621 tttgctaagt ggcttgtggc ttgatcccac tcatccaaaa gcacctccca catagatagt
     1681 tcttctccca gcaaagacag gtagactcta tccaatttag ttgtgtcaag aaacttgcct
     1741 ttgaacagcc catcttgcac ccattgagta atggccactt gcctgccttc agaaacagca
     1801 gaaagctgct cgactcgtgg cgcaataaac actcccaaga acaagcccga agaagcgata
     1861 ttcgcgccgt agggaggccg gcagaggagt cttagtacct gtccaacatt cagcctcttt
     1921 ttctctaaag acaacattcc gtcgaatttc tccgtgagag tccgaa
//
LOCUS       DAOQUC010000824         1964 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_74524_length_1964_cov_3.214772, whole genome shotgun sequence.
ACCESSION   DAOQUC010000824 DAOQUC010000000
VERSION     DAOQUC010000824.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1964)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1964)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1964
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74524_length_1964_cov_3.214772"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..785)
                     /locus_tag="PKH07_13035"
     CDS             complement(<1..785)
                     /locus_tag="PKH07_13035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35908.1"
                     /translation="MSRRAWLPFMILCAVVLLFAGCSKDRTATGYVHRLETEEEPNRL
                     LELYEEFLARYPEHATNPAFEKPLGEARERLAALLASKTIARYTPFIEEYLLDHPQSE
                     HMSGLVRRVAEEKTLANLESTLSARQMSPLPKEIRTEIVRLVVESHAESRSHEALGRA
                     LDLLALDFPGLQHFLSLSNALRPLFEEKGQVQYAEILMAAGDFARFGCASPELAVTLY
                     SEAVSRDPSLHEEAAFRSAIACARTDTPIRVNDATVSFDDLLA"
     gene            complement(778..>1964)
                     /locus_tag="PKH07_13040"
     CDS             complement(778..>1964)
                     /locus_tag="PKH07_13040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35909.1"
                     /translation="INMRPSTMALFPDRPWTVFNWIELNIYFGMIGISYILTTEMGFS
                     LWFFWLAQRFQMFSRIMVGAPEAWGIFGLQAIGAIISLGLVYVWSGRTHLRQVVRKAV
                     FNDPNIDDSREPISYRASVVIIVGGFLFLLIWFSLIGLSPLWAFLMMAFYFLTIIVLT
                     RVVSEAGTFVWWAPLYPQFFPIRFFGTALVGSKNMTLLLMTGWNLHDTATCVLPQALN
                     GFKIAEESGLNRRKVFIAMCCALLVSVLACNIPSIYCMYRYGIPNLGWWSRGAPRALA
                     DTISGTLRSKNQLFQPWEFGHITAGGLFTLCLVIMRRHFIWWPFHPLGYVASWCMGRY
                     WFAILIGWSFKVAVTRIGGIRLWKKYYPAALGLVIGETLILFTWLLFHFLKPIHGVLI
                     IE"
BASE COUNT          514 a          516 c          528 g          406 t
ORIGIN      
        1 tgtgcaagga gatcatcgaa actgactgtt gcatcgttta ctctgatcgg agtatcggtg
       61 cgtgcacacg caatggcgga gcgaaaagcc gcctcttcgt gcagagaggg atcacggctg
      121 actgcttctg aatagagggt gaccgccagt tctggactcg cacaaccaaa tcgggcgaag
      181 tctcctgcgg ccatgagtat ctcagcgtat tgtacctgcc ccttttcctc aaacagtggc
      241 cgcaaggcat tggagagaga taggaaatgc tgaagtccag ggaagtccaa cgcaagcaga
      301 tcaagtgccc tgccgagcgc ctcatggctt ctggattctg cgtgagactc cacgacaagt
      361 cgaacgatct cggtgcgtat ttctttgggc agtggagaca tctgccttgc agagagcgtg
      421 gattcgaggt tggcgagagt cttttcctca gcgactcgcc tgacgagtcc gctcatgtgc
      481 tcagactggg gatgatcgag cagatactct tcaatgaatg gggtgtatcg agcgatcgtc
      541 tttgaagcca gcaaggccgc aagtcgttct ctcgcttcgc caagcggttt ctcaaaggcg
      601 ggattcgtag catgttcggg atatcgcgcg agaaactcct cgtagagttc caacagtcgg
      661 ttcggctctt cctcggtttc cagcctgtgg acgtagcctg tggctgttct gtccttggag
      721 catcctgcaa acaggagcac cacagcacag agaatcatga atggcagcca tgctcgtcta
      781 ctcaatgatc agtactccgt gtattggttt gaggaaatgg aacaagagcc aagtgaagag
      841 aatcagtgtc tcaccgatca ccaaaccaag cgcggcggga tagtacttct tccacagacg
      901 aataccaccg attcgagtga cggcgacctt aaaactccaa ccgatgagaa tggcgaacca
      961 gtatcgtccc atgcaccagc tggccacata accaagcggg tgaaacggcc accagatgaa
     1021 atggcgtcgc atgatcacca aacacagtgt gaacaggcca cctgcagtga tatgcccaaa
     1081 ttcccaaggc tggaacaatt ggttcttgga gcgaagtgtt ccggagatgg tgtctgccag
     1141 agctctcgga gcgccacgtg accaccagcc cagatttggt atcccgtatc ggtacatgca
     1201 gtagatggat gggatgttgc aggccaggac agaaaccaga agagcgcaac acatggcgat
     1261 gaagaccttg cggcggttca gtcctgactc ctcggcgatc ttgaaaccgt tcaatgcctg
     1321 tggcagaacg caggttgccg tgtcatgaag attccaaccc gtcatgagca gcaatgtcat
     1381 attctttgat ccgacgagag ccgttccgaa gaaacgtatc gggaaaaact gtgggtaaag
     1441 gggtgcccac cagacaaacg ttccggcctc cgagaccacc cttgtcaaga caatgatcgt
     1501 caggaagtag aaggccatca tcagaaaggc ccacagcggt gacagcccaa tcagcgagaa
     1561 ccagatcagc aggaagagaa atcctcccac gatgatgacc actgaggcgc gataggaaat
     1621 cggctccctc gaatcatcaa tgttcggatc attgaacaca gcttttcgaa ccacttgtct
     1681 caggtgtgtc cgaccagacc agacatagac aagcccaagg gagatgatgg caccgatcgc
     1741 ctgaagcccg aaaatgcccc acgcttcagg cgctccgacc atgattctgg aaaacatctg
     1801 aaaccgttgt gccagccaga aaaaccacag actgaaaccc atctcggttg tcaaaatgta
     1861 cgagatgccg atcattccaa agtagatatt caactcgatc cagttgaaaa ccgtccatgg
     1921 tctatctggg aaaagagcca tggtgctggg gcgcatgttg attc
//
LOCUS       DAOQUC010000825         1964 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_74530_length_1964_cov_2.962808, whole genome shotgun sequence.
ACCESSION   DAOQUC010000825 DAOQUC010000000
VERSION     DAOQUC010000825.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1964)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1964)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1964
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74530_length_1964_cov_2.962808"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1451
                     /locus_tag="PKH07_13045"
     CDS             <1..1451
                     /locus_tag="PKH07_13045"
                     /inference="COORDINATES: protein motif:HMM:NF025465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PmoA family protein"
                     /protein_id="HNT35910.1"
                     /translation="SHLSPLECLQKLEGRIVTFHFKDLNDFGPSAHDVPWGTGKANVP
                     ELLAEIRRQNIKAVFSIEYEHNWDNSMPEIAQCVEFFEKVAKNIGTVKKTEALLGKIA
                     VQSGKFDRQDTPVSLDLSQLPDPWRPLRLTEVKGDTRIPIPSQMDPSGQPVLWWILNG
                     KTAADSVRTFELYEGDPVASMPVIVRQDEGWLEIVARGARVLRYNNGYVVPPVGTDPR
                     YIRSGYIHPAWSPTGLMVTEDFPADHLHHKGIWSAWTKTEFEGRHPDLWNLKGGTGTV
                     RFAGFDWSASGAIFGGFAADMVHTDLSASPAKEALNERWEVRVWNLGGPDDTFWLWDL
                     TSVQKTAGDSPLLLPDYRYGGFGFRGSVEWVDEKLNLQTSEGKTRVDGHGTKSRWCDM
                     SGPVGQGYAGVTILTHPSNFRFPQPMRIWDKGGTFFCYAPPLDGDFAIEPGKDFVSRY
                     RYLIHDGEADRNTIERFWNDFAEPPVVEASFQ"
     gene            complement(1461..>1964)
                     /locus_tag="PKH07_13050"
     CDS             complement(1461..>1964)
                     /locus_tag="PKH07_13050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35911.1"
                     /translation="SRPGVPTDVDASPALVDLTGNGLPEILVGSDNGFFYCFKYDGSL
                     LWRREIGGELFSSPIVADIDEDGLFEVVFGSATGYLHTLNTEDGSDAFESGATWIGGG
                     ILSTPTWADVDGDGLGEIVIGCFVESTSGAVHGEVSVWKTDAPFLPENNPWPTFRQNN
                     YRTGCVR"
BASE COUNT          458 a          512 c          546 g          448 t
ORIGIN      
        1 gctcgcatct ttcgccactg gagtgtcttc agaaactcga aggccgtatt gtcacatttc
       61 atttcaaaga cctgaacgat tttggcccct cggctcacga cgttccatgg ggcacgggca
      121 aggcgaatgt tccggaattg cttgctgaaa tacgtcgtca gaacatcaag gcagtgttct
      181 cgattgagta tgagcacaac tgggacaact ctatgccgga gatcgctcag tgtgtcgagt
      241 tctttgagaa ggttgccaag aacatcggca cggtgaaaaa gaccgaagcg ctgcttggga
      301 agatcgctgt tcagtcgggc aaattcgacc gccaggatac gccggtgagt cttgaccttt
      361 cgcagcttcc agatccttgg cgaccactgc gccttactga agtcaaaggc gacacgcgca
      421 taccgatccc atcccagatg gatcccagcg gtcagccagt cctgtggtgg atcctgaatg
      481 gaaagactgc tgcagactcg gttcgcacgt ttgagctcta tgaaggagat cccgttgcat
      541 ccatgccagt tattgttcgt caggatgaag gctggcttga gatcgtcgcc cgtggagcaa
      601 gagtgcttcg atacaacaac ggctacgttg tgccacctgt cggtaccgat ccgaggtaca
      661 ttcgaagtgg ttacatacat ccggcgtgga gcccaacggg acttatggtc acagaagact
      721 ttcccgccga tcatcttcat cacaagggaa tctggagtgc ttggacgaag acagagtttg
      781 agggacgaca tccggatctc tggaatctca aagggggaac tggaacggtt cggttcgcag
      841 gttttgactg gagcgcttcg ggagccattt tcgggggatt tgcggcggac atggttcaca
      901 cagacttgag cgcgtctccc gccaaagaag cactgaatga acgatgggaa gtccgtgtct
      961 ggaatctcgg cggaccggat gacacgttct ggctttggga cctgacatcc gtacagaaga
     1021 ctgctggcga cagtcccctt ctcctccctg actatcgcta tggcggtttc ggttttcgag
     1081 gttccgtcga atgggtagat gaaaagttga accttcagac ttcggaagga aagacccgcg
     1141 ttgacggcca tggaacgaag tcgaggtggt gtgatatgag cggtccggta ggtcaggggt
     1201 atgccggtgt gacaattttg acacatcctt ccaacttccg gtttccacag ccgatgcgca
     1261 tctgggacaa ggggggaaca ttcttctgct atgcgcctcc gctcgacggg gactttgcga
     1321 ttgaacctgg taaggatttc gtttcccgct accgatatct gattcatgac ggagaggcag
     1381 atcggaatac tatcgagcga ttctggaatg atttcgcaga accgccggtc gttgaagcca
     1441 gttttcagtg attctctggt tcaccgaaca caaccggttc gatagttgtt ttgtcggaag
     1501 gttggccaag gattgttttc gggcaggaat ggtgcatcgg tcttccagac tgacacttcg
     1561 ccatgaacgg ctcccgatgt tgactcaacg aagcagccaa tgacgatctc tcccaggccg
     1621 tctccatcaa cgtccgccca tgttggcgtt gagagaatgc ctccaccgat ccaggtcgca
     1681 ccggactcaa aggcgtctga gccatcctca gtgttgagtg tgtggaggta accggtagca
     1741 ctgccaaata ccacttcaaa caagccgtct tcgtcaatat cagccacaat gggcgatgaa
     1801 aacagctcac cgcctatctc ccgacgccac agaagcgagc catcatactt gaagcagtag
     1861 aagaatccat tgtccgaacc aacaagaatc tccggcaaac cgttgcctgt cagatccacc
     1921 aacgccggag aagcatccac atcggttgga acgcctgggc gaga
//
LOCUS       DAOQUC010000826         1962 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_74671_length_1962_cov_2.296801, whole genome shotgun sequence.
ACCESSION   DAOQUC010000826 DAOQUC010000000
VERSION     DAOQUC010000826.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1962)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1962)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1962
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74671_length_1962_cov_2.296801"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..646
                     /locus_tag="PKH07_13055"
     CDS             <1..646
                     /locus_tag="PKH07_13055"
                     /inference="COORDINATES: protein motif:HMM:NF018758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35912.1"
                     /translation="SRLEDIRIAYRADDRTALKSICLDLMTDHASTRERIPCLEHFYE
                     RIFQITGIPRRILDVASGVHPLAIPWMGLDCDAVYHAYEIARDMVDGLNGFLEAIGMM
                     PLVHFQDVICVPPEQEGDLAFLMKMVPCLERRQKGIALQLIHSLRVRYVVVTFPVQSL
                     SGRSKNMPEHYTKTFTESLQGTQWKLAHVPMEGELVFVVDKNPTSEKHQQNCHP"
     gene            complement(609..1403)
                     /locus_tag="PKH07_13060"
     CDS             complement(609..1403)
                     /locus_tag="PKH07_13060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011187636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNT35913.1"
                     /translation="MPDSCLSTGCKVSYFAVDSIERGKGVSTMRLLIAAILIVTGLPE
                     TYGTEGVIMLHGLCRTSASMEKMKTALTGAGYKVVNVDYPSRTASIDQLSEKVISKAM
                     DDPVLEGCAKVHFVTHSLGGILVRVYFSKHDTGRLGRVVMLGPPNQGSEVVDNLKEWK
                     AFQKLNGPAGSELGTDQQSKPKTLGAVNFELGVIAGNRSINWINSMMIPGSDDGKVSV
                     EATKVDGMKEHLVLRCTHTCIMRNPKVIEAVTFFLRDGSFAGVSRS"
     gene            1509..>1962
                     /locus_tag="PKH07_13065"
     CDS             1509..>1962
                     /locus_tag="PKH07_13065"
                     /inference="COORDINATES: protein motif:HMM:NF025839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4432 family protein"
                     /protein_id="HNT35914.1"
                     /translation="MSKFFGFGRKQILRRVGLMDQVAGVRRYTLFDGNERDVEAIDFR
                     TGTGFDFTVVPGRGMDITSAWYRGKALAWLSQTGVVHPAYFEPEGLGWLRTFYGGLVA
                     TCGMTTAGAPCTDQGEALGLHGRFSTTPARNVVADTRWEGEDYVLRASG"
BASE COUNT          408 a          511 c          537 g          506 t
ORIGIN      
        1 ctcacgactt gaggatatcc gtattgccta tcgtgccgat gaccggacgg cactgaaaag
       61 catttgtctt gatctcatga ccgatcacgc ttcaacccgc gagcgaatcc cctgcctgga
      121 gcacttttac gagaggatct ttcagattac cggtattccc aggaggattc ttgacgttgc
      181 gtcgggagtg catcctctgg ccattccctg gatgggattg gactgcgacg ctgtctatca
      241 tgcctatgaa attgcccgtg atatggtgga tgggctgaac ggttttctgg aagcgatcgg
      301 gatgatgcct ctcgtccatt ttcaggatgt tatctgcgtt cctcctgagc aagaggggga
      361 tctggcgttt ctgatgaaga tggttccctg cttggagcga cgccagaagg gcattgctct
      421 gcaactcatc cattcacttc gggtgcgcta tgtggttgta acgtttcctg tccagagtct
      481 ctcgggtcgc tccaagaaca tgcctgagca ctacaccaag actttcaccg agagcctgca
      541 aggtacccag tggaaattgg ctcatgtgcc aatggaaggg gaactggttt ttgtggtgga
      601 caagaatcct acgagcgaga aacaccagca aaactgccat ccctgagaaa gaaggttacg
      661 gcttcgatca ctttgggatt tcgcatgatg cacgtgtgtg tacagcgcaa gaccaggtgt
      721 tccttcatcc catccacttt cgtcgcttcg accgagacct tgccgtcatc agaaccgggg
      781 atcatcatgc tgttgatcca gttgatcgat cggttgcccg cgatgactcc cagctcgaaa
      841 ttcacagcgc caagtgtctt gggtttggac tgctgatcgg tgcccagttc tgagccggct
      901 ggcccattga gtttctgaaa ggccttccat tccttcagat tgtcaaccac ttcgcttccc
      961 tgatttggcg gtccgagcat gaccacgcgt cccaggcgac cggtgtcatg tttcgagaaa
     1021 tagacgcgta ccaggattcc accgagcgag tgagtgacaa agtgaacctt ggcgcatcct
     1081 tcgagcacag gatcatccat ggctttgctg atcaccttct cgctcaactg gtcaatgctt
     1141 gctgtacgcg atggatagtc cacgttgacg actttgtatc cggcacccgt gagtgcggtc
     1201 ttcattttct ccatactggc acttgttcgg cacagaccgt gcaacatgat gacaccttct
     1261 gtgccgtatg tttccggaag cccggttacg atcagaatgg ctgcgatcaa caagcgcatc
     1321 gtcgagacac cctttcctcg ttctatgctg tcaactgcaa aataacttac cttacagcca
     1381 gttgacaggc aactgtctgg catcccaatt ctcttttgtc tgacggtgtt tcctcttttc
     1441 cctgaaccgc gtttgtggta agaacaccct ataaagagtg agaaaacctt attcaggggg
     1501 tctttaacat gtcgaagttt ttcggttttg gcaggaagca gatactaagg cgcgtcggtc
     1561 tgatggatca ggttgcgggc gtgaggcgtt acaccctgtt cgatggcaat gagcgtgacg
     1621 tcgaagcgat tgatttcaga acgggcactg gctttgattt caccgtcgta ccaggcagag
     1681 gcatggatat caccagcgcc tggtaccgcg gcaaggcgct ggcatggtta tctcagaccg
     1741 gtgtggtgca tccggcgtac tttgagccgg aaggactcgg ctggttgcga acgttctatg
     1801 gtggactcgt tgccacatgt gggatgacga ctgccggtgc gccttgtacc gatcagggtg
     1861 aggcgttggg gcttcatggg cgattctcga cgacccccgc caggaatgtc gttgctgaca
     1921 cccgatggga aggagaagac tatgttcttc gtgcctcagg ac
//
LOCUS       DAOQUC010000827         1961 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_74702_length_1961_cov_3.573452, whole genome shotgun sequence.
ACCESSION   DAOQUC010000827 DAOQUC010000000
VERSION     DAOQUC010000827.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1961
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74702_length_1961_cov_3.573452"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /locus_tag="PKH07_13070"
     CDS             complement(<1..378)
                     /locus_tag="PKH07_13070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011153892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic/DOC family N-terminal domain-containing
                     protein"
                     /protein_id="HNT35915.1"
                     /translation="MAAEAQCSIVCRGGRYVQQPTGYRAFIPNPLPPDPPIEMKVELQ
                     ALLSSADRALGRLDGSIYTLPNPDLFVAMYVRKEAVLSSQIEGTQSSLQDVLAAEAKA
                     LSSKNPSDVAEVFNYVAAMNHGLE"
     gene            complement(623..763)
                     /locus_tag="PKH07_13075"
     CDS             complement(623..763)
                     /locus_tag="PKH07_13075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35916.1"
                     /translation="MIEEFEEMEDEIEFLDLSMDDLLPIIEEIEEEEIESEENDLPMQ
                     RS"
     gene            complement(842..>1961)
                     /locus_tag="PKH07_13080"
     CDS             complement(842..>1961)
                     /locus_tag="PKH07_13080"
                     /inference="COORDINATES: protein motif:HMM:NF024810.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HNT35917.1"
                     /translation="EDDGVLGDLYRFDPSSLHSWLITSVTSPPRMFAHGAAAKSVTEM
                     LIYGGYRVTEDSEAEASRLWVYDATASLWSSSRFVSSVTNLWNYFPGIQYFEESDAAY
                     ITGGMYTDSPLDYQPEIRTATEMYAIQMTNLFRRLDWKNKQLESRATKPGLASLGSYT
                     AMNNGTLYVYGGVAPCGSPLADLWYYNTTYNYWMMGNQNPTIIPRSFGTVQSLTDTLF
                     VSGGYKYNIGAAPSLQATDVPTGETWLYNTKTSAWTRCADGPPLTYAQSALVKVNGQD
                     VIILFGGILNGAPNSAFYVYYFGDTPPYQDQPTPTPTPTTRPGDYDADGFVDSRDLSR
                     LVEKFAASSPEVDLNGDGKVDALDLFLFSLNWEPTTGG"
BASE COUNT          453 a          532 c          493 g          483 t
ORIGIN      
        1 ttcaagccca tggttcatgg cagcaacgta gttgaaaacc tcggcaacat cgctcggatt
       61 cttgctactg agtgctttgg cttctgcggc aagcacatcc tgaagagagc tttgcgtacc
      121 ctcaatctga ctggagagaa cagcttcctt gcggacatac atggctacaa acaagtcggg
      181 attaggaagc gtatagatgg aaccgtcaag tcggcctaga gcacgatcag cacttgacaa
      241 cagggcctgc agttcaacct tcatttcgat tggtgggtca ggtggcaacg gatttgggat
      301 gaaagcccga tagccagtcg gttgctgcac atagcgcccc cctcggcata cgatgctgca
      361 ttgtgcttcc gccgccatgc ttaactccca gtttagtgta acgccgatcc gccgccatat
      421 taaagtctac gtttactata ggagaaatcc cacctatatg caagacttct ttcatgactt
      481 ctttcatgac tttgtctcga gtaccaagtc atgaatcact tcccggcaac tcagtgagtg
      541 gctctggggg atagggattc ctcttgctaa cgcgaacccc gagtcagagt tagatttcgg
      601 cggtcagtct tgagcaaggc attcatgacc tctgcatggg aaggtcattc tcttcagact
      661 cgatttcctc ttcctcgatc tcctcgatga tgggaagcaa gtcatccatc gaaagatcaa
      721 ggaattctat ctcgtcttcc atctcctcga actcttcaat catcttcaac acccccttgt
      781 agagatgact cagaaaaggc tccttccgtt cttacgcgcc gcagcgctga gtattcagag
      841 ttcaccctcc tgttgtcggt tcccagttga ggctgaacag gaagagatcc agggcgtcca
      901 cctttccgtc cccgttcaga tcgacttctg gcgaagatgc ggcgaacttc tccacgagtc
      961 gagagagatc tcttgagtca acaaacccat ccgcgtcata gtcacctggt ctggtcgtag
     1021 gagttggtgt tggtgtcggt tgatcctgat acggaggcgt gtcgccgaaa tagtacacgt
     1081 agaaagcgct gttcggagcg ccgttcagta tgcctccaaa caggatgatc acatcctgcc
     1141 cattgacttt caccaaagcc gactgagcat aagtcagggg cggaccatcg gcacaacgtg
     1201 tccaggcgga tgtcttcgta ttgtagagcc aggtttctcc cgttggcaca tccgtcgctt
     1261 ggagactggg tgccgcacca atgttgtact tgtagccccc cgaaacaaac agagtatcgg
     1321 tgagagactg cacggtgcca aaagaacggg ggataatcgt cgggttctga ttgcccatca
     1381 tccagtagtt gtacgttgtg ttgtagtacc acagatcagc caggggggaa ccacatggcg
     1441 caacaccccc atagacgtag agagttccat tgttcatcgc tgtgtacgag ccgagtgaag
     1501 caagcccggg cttggttgcc ctgctctcca gctgtttgtt cttccagtcg agtctgcgaa
     1561 acaagttcgt catctgtatg gcatacattt ccgtcgccgt gcgaatctcc ggctggtagt
     1621 ccagtggcga gtccgtatac atcccgcctg tgatataggc tgcgtcggat tcctcgaagt
     1681 actgaatgcc aggaaagtag ttccagagat ttgttacaga tgagacaaat ctggaagaac
     1741 tccacagact tgccgttgcg tcatacaccc aaagcctgct tgcctcagcc tctgaatctt
     1801 cggttactcg gtagcctcca tagataagca tctcggtgac gctcttggcc gccgctccat
     1861 gtgcaaacat ccttggcgga gaagtcacac tcgtaatcag ccaggaatgc agtgagcttg
     1921 gatcaaaccg atacaagtct ccgaggacac cgtcgtcctc a
//
LOCUS       DAOQUC010000828         1961 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_74730_length_1961_cov_2.486359, whole genome shotgun sequence.
ACCESSION   DAOQUC010000828 DAOQUC010000000
VERSION     DAOQUC010000828.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1961
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74730_length_1961_cov_2.486359"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..595
                     /locus_tag="PKH07_13085"
     CDS             <1..595
                     /locus_tag="PKH07_13085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35918.1"
                     /translation="PEEKRVEVYYALGGGTKGFSYWWYTPYGNFHGCGSSDPSGVSLY
                     NEIARLGAEVRTAAPLILRSCPAGLDVEKPKNLSVWPLLVGRDSLVLLNINEQICSDR
                     LGTVVVQQEDVKSLVRIPSWLKPADVFEISSSGISDATWTIENSDLSVDLGTVFLTRM
                     IVVTSDASLRATLQGLYKERFEGNIKSLPEVTLPEQP"
     gene            complement(643..1671)
                     /locus_tag="PKH07_13090"
     CDS             complement(643..1671)
                     /locus_tag="PKH07_13090"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNT35919.1"
                     /translation="MKTLRTALIGLGRIGWKFHLPNIVRHPGFELIAVVDPSEERLKE
                     ADTEYRVKGYQDYRQLLDSEKLDVVVIASPTPFHADQAIASFDSGCDVFCEKPLTASL
                     QDTDRVIASMRSNHRKLMVYQPHRGMAPVVALRDVLKEDLIGRIYLIKRTHTGYQLRN
                     DWQAFRKYGGGMLNNYGAHFIDQLLSLSGRNVKKASCWLRSEASLGDAEDVVKALLEC
                     DDGLLLDLEINMATAVQMPPWFILGKTGAIVQDEQNKSWKIRYFRDSELGEIATQEGL
                     AAEQRSYGCGVTIPWREVLRSYEHYAPVDHYQKLYEHIALNRDPFVPIEQTRELMRVI
                     EECRRVAE"
     gene            complement(1718..>1961)
                     /locus_tag="PKH07_13095"
     CDS             complement(1718..>1961)
                     /locus_tag="PKH07_13095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional homocysteine
                     S-methyltransferase/methylenetetrahydrofolate reductase"
                     /protein_id="HNT35920.1"
                     /translation="GILPLSSSRNAEFLHHEVPGMHIPQDIRDRMERVGSGPKAKAEG
                     VAIAREALEQCRGLSQGVYVMPPFNNVQAALDVLKD"
BASE COUNT          453 a          515 c          515 g          478 t
ORIGIN      
        1 tcccgaagag aaacgggtag aagtctacta cgcgcttggt ggtggcacca aaggcttctc
       61 ctactggtgg tacacgccgt acggcaattt ccatgggtgt ggcagctcag atccaagtgg
      121 agtctctctt tacaatgaga ttgctcgctt aggtgcggaa gtgcgaaccg ccgcgccttt
      181 gattcttaga agctgtccgg caggactgga cgtcgagaag cccaagaacc tcagcgtctg
      241 gcctctgctg gtgggcagag attcgcttgt tctgctgaac atcaacgagc agatatgcag
      301 tgacagactt ggaacggttg tcgttcagca ggaagatgtg aaatccttgg tgaggattcc
      361 ctcatggctc aaacctgctg atgtctttga gatttcgtcc agtggaatct ccgatgcgac
      421 gtggacaatc gaaaactcag atctgagtgt cgatctggga acggtatttc tgactcgcat
      481 gattgttgtc acatcggatg cctctcttcg ggctactttg cagggccttt acaaggaacg
      541 ctttgaggga aatataaaga gtctgcctga agtgacactt cccgaacaac cgtgacaggt
      601 tgatggcgag agcctgacac ggtttgacgc agcaagcgga tcttattcgg caacgcggcg
      661 gcattcctcg atgacacgca tgagttccct tgtttgttcg atggggacaa aaggatccct
      721 attcagtgcg atgtgttcat acaacttctg atagtggtcc acgggagcat agtgctcata
      781 cgagcgcagc acttctcgcc agggaatggt gactccacat ccgtagcttc gttgctcagc
      841 ggcaagaccc tcttgcgtcg caatttcgcc cagctcagaa tccctgaagt accgaatctt
      901 ccaggacttg ttctgttcat cctgaacaat cgcacccgtc tttcccaaaa tgaaccaagg
      961 aggcatctga acggctgtcg ccatgtttat ctcaagatca agaagcagcc catcatcgca
     1021 ttccaagaga gccttcacaa cgtcttcggc atcgccaaga ctggcttcag atctcagcca
     1081 gcatgaggct ttcttgacgt ttctgccgga aagtgagaga agctggtcaa tgaaatgtgc
     1141 tccatagttg tttaacattc caccaccata tttgcggaaa gcctgccaat catttctcag
     1201 ctgatatcct gtgtgcgtcc gcttgatgag atagatgcgc ccgatcagat cctccttaag
     1261 aacgtcccgc agggcaacga ctggtgccat gccacggtga ggctggtaaa ccatgagctt
     1321 gcgatggtta gatcgcattg atgcgatgac gcgatccgtg tcttggagcg atgcggtcag
     1381 aggtttctcg caaaacacgt cacatccgct gtcgaaagag gcaatcgcct gatcggcatg
     1441 aaagggtgtg ggagacgcga tgacaacgac atcgagtttc tcggagtcca gtagctggcg
     1501 gtagtcctgg taccccttaa ctctgtattc cgtgtcagcc tccttgagtc tctcttcaga
     1561 aggatcaaca actgctatca gttcaaaacc aggatgtcga acaatgttgg gaagatgaaa
     1621 cttccagcca atacgtccca aaccgatcag tgcagttctc agcgttttca cgattctctc
     1681 ctgtgcacgg catggatatt catcatccca gcgacctcta gtctttcagc acgtccaacg
     1741 cggcctggac attgttgaaa ggcggcatga catagacgcc ttgcgacaga ccgcggcact
     1801 gctcaagcgc ctctctggca atggccacgc cttctgcctt ggcttttggc cctgagccga
     1861 ctcgctccat tctgtcgcgt atgtcttgag gaatgtgcat tccggggacc tcatggtgaa
     1921 gaaactccgc atttcgggag ctggacaggg gcaggatacc a
//
LOCUS       DAOQUC010000829         1956 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_75013_length_1956_cov_3.709627, whole genome shotgun sequence.
ACCESSION   DAOQUC010000829 DAOQUC010000000
VERSION     DAOQUC010000829.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1956)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1956)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1956
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75013_length_1956_cov_3.709627"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            259..>1956
                     /locus_tag="PKH07_13100"
     CDS             259..>1956
                     /locus_tag="PKH07_13100"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNT35921.1"
                     /translation="MTPKMLSFRILVVGMLCLWFVLAKELGFMKSPFKLPCLAYVFFS
                     LLATFRAVSMFECFGSEYLPHVIALSFFVILVHKLIQGNTRLVSLSILIVAVIGLLTG
                     IYGIHQGLGFVDVFIGQRLVGAEQGPVSFMGNTNFAANYLLLALPVVSMMLLAIDGGA
                     RISLMLMPVVTSSMIYGYLKFPWALHFTLVGTVGILILCFRTPNVPKAMLYALSSITM
                     SVYMGFTQARFANMGTMLAMGVMLAIGLYLRFIPWMCHFKFGQSKSSLPANLFTGPFL
                     SMILVAFLLAFDLVAIRFKTDRSEMMQWGMNLSSGLFLFALLTLFLQTLRFPLSEAAR
                     AAAASDKTPLPRSTPHQNDQRNLPDVLARWQTTYRKVVLVCLVIGIIAGLAVAPRVHQ
                     AIIKVPSFVFRLECWRSAGRTIADNFFLGIGPGHFKIIHPIYTSHLENSVLGVERLAR
                     KVHNDFMAVWIEYGLGAMIAFFWFLVLIGMMLFYIFRRLDVFKTNPEVFDAQQTRYHF
                     YLALGLVGAFVGTLFQANGESNFLQPASTIQIWFFAGLIASIYLRLRPGNGSFLDRIS
                     "
BASE COUNT          391 a          533 c          458 g          574 t
ORIGIN      
        1 gagcctctgc aaccgaagca tacctctacc ccctccgtaa ctgagccgac tcattccagt
       61 tgggaaatgt cttgcggaat atcgtcatta acgtatagaa tacgcccttg ttagagttgg
      121 tacgctctgt gtttggaggt tcgcgtcaca ttggaatatc aagtacgccg attagggatg
      181 ctgtcgcttc gacttctcgc attcacaacg atagtagttc ctcccctgat tttttgcctt
      241 tatacctggg agcactatat gactcccaag atgctcagtt ttcgaattct ggttgtcgga
      301 atgctctgcc tgtggttcgt tcttgccaag gaactgggct ttatgaagtc tccgttcaaa
      361 ctgccgtgtc tcgcctacgt attcttcagt ctgctggcaa cgtttcgtgc cgtcagtatg
      421 tttgagtgct tcggttctga gtacctgcca cacgtcattg ccctctcgtt cttcgtgatc
      481 cttgtacata agctcattca gggcaatact cggttagttt cgctttcgat acttattgta
      541 gcggtaatag ggcttctgac tggcatctat gggattcacc aaggtcttgg atttgtagac
      601 gtcttcatcg gacaaagact cgtgggggcg gagcaagggc cggtgtcatt catggggaac
      661 accaacttcg ccgcaaacta tctcctcctt gcgttgccag tggtttccat gatgcttctg
      721 gccatagacg gcggagcacg gatcagtctc atgctgatgc cagttgtgac atcctccatg
      781 atctacggct atctgaagtt cccctgggca cttcatttca cgctcgtcgg cactgtggga
      841 attctgattc tctgctttcg aactcccaat gttcccaaag caatgctcta tgccctttcc
      901 tctatcacca tgtcggtcta catgggtttt acacaggcgc gtttcgccaa catgggaacc
      961 atgcttgcca tgggcgtcat gctcgcaatt ggtctttatc tgagattcat cccgtggatg
     1021 tgccacttca aatttgggca gagtaagtca tcactgccag ccaatctgtt caccggaccg
     1081 ttcttgagca tgattctggt tgcttttctg ctggcattcg atctggttgc cattcggttc
     1141 aagaccgatc gttctgagat gatgcaatgg gggatgaatc tctcttcggg gctttttctc
     1201 tttgcgctcc tgacactctt cctgcaaaca ctaaggttcc ctctctcaga agcagcccgg
     1261 gcagccgctg cctcggacaa aacaccacta ccgagatcaa caccccatca gaacgatcag
     1321 agaaatctgc ccgatgtact tgctcgctgg cagaccacct atcggaaagt cgtcttggtt
     1381 tgtctagtca tcggaatcat cgccgggctt gccgtcgcac ctcgggttca tcaggccatc
     1441 atcaaggtac cctcttttgt cttccgcttg gaatgctggc gtagcgcagg ccgaacaatt
     1501 gctgacaatt tcttcctagg cataggtcct ggacacttca aaatcatcca tccgatttac
     1561 acttcgcatc tggaaaactc ggtgctgggc gtggaacgcc tcgcacgcaa agtgcacaac
     1621 gacttcatgg ccgtctggat tgagtacggc ttgggagcca tgattgcatt cttctggttc
     1681 ctcgttctca ttggaatgat gttgttctac attttccgac gcctggatgt tttcaagacc
     1741 aaccccgaag tgtttgacgc acaacagaca cgctatcact tctatctggc gctcggtctt
     1801 gtcggcgctt tcgtagggac cctgtttcag gcaaatggcg agtccaactt tctccaacct
     1861 gcatccacca ttcagatctg gttctttgca ggtctgatcg cttcgatata tcttcgcctg
     1921 cgacctggaa atggatcctt tctggatcgc atttct
//
LOCUS       DAOQUC010000830         1955 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_75089_length_1955_cov_2.986316, whole genome shotgun sequence.
ACCESSION   DAOQUC010000830 DAOQUC010000000
VERSION     DAOQUC010000830.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1955)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1955)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1955
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75089_length_1955_cov_2.986316"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..730
                     /locus_tag="PKH07_13105"
     CDS             <1..730
                     /locus_tag="PKH07_13105"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HNT35922.1"
                     /translation="SPLVGVACVVCAVMLIPTLGNRALFAFQGDIIFMAYLLGLMRFF
                     LVVAALDTGSSFEGMGASREVTFSAIAEPALLLGMAVLAKLSGTMSLSGMFAGLNSAA
                     WLAAGPALAVTVASFLIILLSENARIPVDDPNTHLELTMIHEVMVLDYGGPDLGVILY
                     TASLKLWIFSALIVNTVLSCLEMKGLTAVGAFAGGMILVAILVGVIESVMARLRLLTV
                     PRLLSSAVVFSILAFILVLRLSGR"
     gene            727..1356
                     /locus_tag="PKH07_13110"
     CDS             727..1356
                     /locus_tag="PKH07_13110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase"
                     /protein_id="HNT35923.1"
                     /translation="MITLLDSILFLLILTDFVLLGSSRLRTCIKMIAVQGVITGLLGV
                     ATSESMLSLGILTIALKGFVFPYLLLRAMSASRTKREVDPFLGYSASLFAGILCIIVA
                     FWLDGRLHLPVSTVSKLMVPVAFSTIMIGLLLIIARKTAISQVLGYLALENGIYIISV
                     GIVQETPFLIELGVLLDVFVGVFVMGIAIHRISREFDHIDSDQLSTLRG"
     gene            1454..>1955
                     /locus_tag="PKH07_13115"
     CDS             1454..>1955
                     /locus_tag="PKH07_13115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35924.1"
                     /translation="MAFCAVGTTLVAILGGYATTLVFTRGTLFDAHRWFMLDALSGYH
                     LIVMLIVFWLSSLYCMCYFRLEIEKETFDTKTARRFGSLWFGSMAAMLLVLVSNNLGI
                     MWVGIEATTLLTAFLICIHTSPASLEAMWKYLLMCSVGVALAFTGTLFIAASTKAAGL
                     HGSEALL"
BASE COUNT          353 a          503 c          501 g          598 t
ORIGIN      
        1 cagccctctg gtcggtgttg cctgtgttgt ctgtgccgtt atgctcattc ccactcttgg
       61 caatcgagcg ttgtttgctt tccagggcga tatcatcttc atggcatatc tacttggatt
      121 gatgcgtttc ttcctggtcg ttgctgcgct tgacacgggc tccagtttcg agggaatggg
      181 agcaagtcgc gaagtcacct tctccgccat tgcggagcct gcactgcttc ttggtatggc
      241 cgttttggcg aagttatctg gaaccatgag cctttcggga atgtttgctg gtttgaactc
      301 agcagcgtgg ctcgcagcag gacccgcatt ggcagtgacc gtggcgtctt tcttgatcat
      361 ccttctctcg gaaaatgcgc gcatcccagt tgacgatccg aacacacatt tggaactgac
      421 catgattcac gaagtgatgg ttctggacta cggtggaccg gatcttggcg tgattctcta
      481 caccgcttct ctgaaattgt ggatatttag tgcgctgatc gtgaacacgg ttttgtcgtg
      541 tcttgaaatg aagggcttga cagcagttgg agcatttgcg ggagggatga tccttgtggc
      601 gattctcgtc ggagtcatcg aatctgtgat ggcccgcctt cgccttctga ctgttcccag
      661 actgctctcc agtgcggtcg tcttttcgat tcttgccttc atcttggtgc tgaggttgtc
      721 gggaagatga ttactctgtt ggactcgatt ctgtttctgc tgatcctcac cgacttcgtg
      781 cttcttggga gcagcaggct cagaacatgc atcaagatga ttgcggtgca aggcgtcatc
      841 acaggtctgt tgggagttgc gacaagtgaa tccatgttat ctctcggaat cctgactatt
      901 gcactcaagg gcttcgtgtt tccttatctt ctcttgagag ccatgagtgc atcccggaca
      961 aagagagaag ttgatccttt ccttgggtac tccgcatcac tgtttgcggg catcctatgt
     1021 attatcgttg ccttctggct tgatgggaga ctccatctgc cggtcagtac tgtgtcgaaa
     1081 ctgatggttc ctgttgcctt ctccacaatt atgattggac tgctcttgat tatcgcccgc
     1141 aagactgcca tcagtcaggt gttgggatat cttgctcttg agaacggcat ctatatcatc
     1201 agtgtgggaa tcgttcagga aaccccgttt ctgattgaac tcggagtcct gttggatgtc
     1261 tttgtcggcg ttttcgtcat gggtattgcc atccatcgga tcagccgtga gttcgatcat
     1321 attgactctg accaactatc cactttgaga ggctgacgca acaatgaaca cattgcatcc
     1381 gttgccactg ctcgttttgt tgcctgtact attcagcctg atctgcctca ctctttcctc
     1441 cccacgatct atcgtggctt tctgcgcggt gggcacgaca ttggtcgcca tcctcggtgg
     1501 ctatgccaca acgcttgtgt tcactcgggg cactctattt gatgcgcacc gatggttcat
     1561 gcttgacgcc ctctcgggct accatctgat cgtcatgctc atcgtcttct ggcttagttc
     1621 tctgtactgc atgtgttact tccgcctgga aattgagaag gagacgttcg acacaaaaac
     1681 ggcacgacga ttcggatctc tatggtttgg ctccatggct gcaatgttac tggtgttggt
     1741 gtccaacaat cttggcatta tgtgggttgg aatcgaagcc acgacgcttc ttacagcttt
     1801 tcttatctgc attcacacat ccccagcttc tttggaagcg atgtggaagt atcttctcat
     1861 gtgctctgtc ggcgtcgcac tggctttcac tggcaccctg ttcatcgccg cctcaaccaa
     1921 agcagccggc cttcatgggt ccgaggcgct ccttt
//
LOCUS       DAOQUC010000831         1954 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_75142_length_1954_cov_3.416535, whole genome shotgun sequence.
ACCESSION   DAOQUC010000831 DAOQUC010000000
VERSION     DAOQUC010000831.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1954
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75142_length_1954_cov_3.416535"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..365)
                     /locus_tag="PKH07_13120"
     CDS             complement(<1..365)
                     /locus_tag="PKH07_13120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35925.1"
                     /translation="MQRFRFSILILGILIAPSLWAQDYVLERFWSVADTPGGIGVFDG
                     DSFFISNTDGGSIRKWTSGVGESQVTTWVNPSNPSDSLAMVGGVSTGPRGISIDPFSN
                     VLTLSNDTDHNVLVWNLNL"
     gene            831..>1954
                     /locus_tag="PKH07_13125"
     CDS             831..>1954
                     /locus_tag="PKH07_13125"
                     /inference="COORDINATES: protein motif:HMM:TIGR01596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
                     /protein_id="HNT35926.1"
                     /translation="MGDDSENAMKFLAHSASDTGHVDSVRSHLSEVARCAAEFAGAFG
                     AQEEARNAGLLHDLGKYGDLFQKRLRHETSGVDHWSLGAFQALMHAKALGIASALAIQ
                     GHHIGLQWASQDSLRNLSPEFLMQGHPLGLRIPDESLSVLLKRFEDDGLALSKIDASV
                     FGYSSLCSECADAMLDVRMLFSTLVDADFIETEAHFQATDVDHKCYRRKGLPLEPKTA
                     LDELMMYLDHLRKSGTSSLEIQRVRDDLLQACLCAAEQPSGIFTLTAPTGSGKTLSMM
                     AFALRHALEHNLSRIIVVIPYLSIIEQNVAVYQQVFSRILSEADLKQYVLEHHSMSGT
                     REGEEAYESQGRLLSENWDAPIVVTTSVQFLESLFSNRPGA"
BASE COUNT          459 a          485 c          510 g          500 t
ORIGIN      
        1 tccaggttca agttccagac caaaacgttg tgatcggtgt cattggacaa tgtcagcaca
       61 ttggaaaatg ggtcaatact gattccacgg ggtccagtcg aaacgccacc gaccatcgca
      121 agagaatcgg atggattgga cgggttcacc caggttgtga cctgtgactc accgactccg
      181 ctcgtccatt ttcggatgct tcctccatcg gtatttgaga tgaagaacga atctccgtca
      241 aacactccaa tacccccggg agtgtcagct accgaccaga accgttccag gacataatcc
      301 tgtgcccaga gtgaaggggc gatgagaatc ccaaggataa gaatagagaa tcgaaatcgc
      361 tgcataatag tggcaacctc cagtccataa cattaagcaa aagtcttgcc cgattatccc
      421 acatccgtca acggaataat gcgatacggt ttcgcgccct gtccgtctcc gtagttcaag
      481 gtgaagtgcc ttctgtgcag aagccttttg cctttgttca gagcctgtca cgagtctgtc
      541 gcaggagaga cgccgagtcc agtttctccg aacgctgaaa aaagatttgc atccgctcca
      601 ggcaggggta aactgatatt ggctagaacg cttatgacag caagagaacc cgagattttg
      661 tgaaggcaag ccggtttccg accggcttcg ccgttttggg gagggaggta gtttttgcca
      721 ccgagttgct gtgttgacac tagaggccaa tcccttctat attgcattta ctcacacctt
      781 tgcagggcga catccatctg agttcctgca aatctctgag ggaggagaca ttgggagacg
      841 actccgaaaa tgcgatgaag ttccttgccc actccgcttc ggacacgggt catgtggatt
      901 cagtgcgatc acacctgtct gaagttgccc gatgtgctgc agaatttgcg ggtgcttttg
      961 gggcgcaaga agaagccagg aatgctggtc tactacatga cttggggaaa tacggagatc
     1021 tatttcagaa gcgtcttcgt catgagactt ctggcgttga tcattggtca ttgggtgctt
     1081 ttcaggctct catgcacgcg aaggccttgg gtattgcatc agctcttgcg atacagggac
     1141 atcacatcgg cctgcagtgg gcttctcaag attcgttgag aaatctctct ccggagtttc
     1201 tcatgcaggg acatccactt ggattgagaa tcccggacga gagtctttcc gttctcctga
     1261 agcgttttga ggatgatggt ctcgcacttt caaagattga tgcatccgta tttggctact
     1321 cctccctttg cagcgagtgc gccgatgcca tgcttgacgt tcggatgctg ttttcgacct
     1381 tggttgacgc tgacttcatt gagacagaag cgcatttcca ggccactgat gtagatcata
     1441 agtgctatcg aagaaaggga ttgccactgg agccgaagac agctcttgat gagctgatga
     1501 tgtatctcga tcacctcagg aagtcaggta ccagttcgct tgagatccag agagttcgag
     1561 atgacctgct ccaagcctgc ctgtgtgcag cggaacagcc ctctggtatc tttactctca
     1621 cagcgccgac gggctcaggc aagacgctga gcatgatggc gtttgctctc agacatgcgc
     1681 ttgagcacaa tctttctcgg attatcgtag tcatccccta tctgagcatc attgagcaga
     1741 acgttgcagt ctaccagcag gtcttctcaa ggattctttc tgaagccgat cttaagcagt
     1801 atgttcttga acatcacagt atgtctggga ctcgggaagg cgaagaagcc tacgaatcgc
     1861 aggggcgact tctgtctgag aattgggatg ctccgattgt ggtcacaact tcagtgcagt
     1921 tccttgagtc attgttttca aaccgtccag gtgc
//
LOCUS       DAOQUC010000832         1949 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_75454_length_1949_cov_3.831045, whole genome shotgun sequence.
ACCESSION   DAOQUC010000832 DAOQUC010000000
VERSION     DAOQUC010000832.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1949
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75454_length_1949_cov_3.831045"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1949)
                     /locus_tag="PKH07_13130"
     CDS             complement(<1..>1949)
                     /locus_tag="PKH07_13130"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNT35927.1"
                     /translation="DEIGRNTRRHVLQEHRLDLAASRYREFISSLEQANGSATPQANV
                     TTDIHPGQLPSVYVVVLNYNGAKHIDVCLESLLAQSYPNTNILMVDNASPDGSGEYAQ
                     KKYPQVESILCKENLGFARGNNLGFDHVLAQGADYVIALNNDVEVDRDWVTRQVEAMQ
                     SDPGIGACGSRILSYWQRDIVNSLGHEMNQWGHTWDIGFGRRFDPNRWGKQQEIVSIC
                     GCSFMIRREVLEKVKGFDPKYFAYYDDVDLSIRIRGLGYRLVYVPDAIVYHKFSASFG
                     HESPRKLALCTVNKWRFMFCHFPLAKTLSVFPRQFKRDAREALRLLRLGESRNTEVLL
                     KALWQFLKMFPGLILYRLKNRWNRQHYSFLKMCVPTNQVPHFFPPRQDYELLREKSDK
                     APSRIMMGHSDDFLGEGWFPAESGYYSEAVRWMSKSASAFLKSSPEAGRNRVLQLHVR
                     CPHAFLSASVNSASKVFPTLAVFVEGERVGEYPIGIEWRTIQCSLGDDLPDVVRVDLG
                     CDPLLTAEHTGELRDVTVQLDEISLLDQDSPLLRRTVDCISSGAVVSASSVAPNLLRA
                     DIHLDEFPIKAVEPSAPFEIAITVRNTGDTCWIAQPLAKGGYVTMGIQLLDADGVLIN
                     ADFGRQVLSESLFPGACAELAVSLCA"
BASE COUNT          496 a          550 c          465 g          438 t
ORIGIN      
        1 gcacaaagtg aaacggccag ttcagcacac gcccccggga aaagagattc agacaacact
       61 tgcctgccaa agtccgcatt gatcaaaact ccatcggcat ccaaaagctg aatacccatc
      121 gtgacgtatc cgccctttgc taaaggctgg gcaatccagc atgtgtctcc tgtgttgcga
      181 acggtaatgg caatctcaaa tggggcgctc ggttccaccg ccttgatcgg aaactcatcc
      241 aggtgaatat ccgccctgag aagatttggc gcaacactgc tggcggaaac caccgcacct
      301 gatgaaatgc agtccacggt tcggcgaagc aggggagagt cctgatccag aagagatatc
      361 tcgtccagct gcaccgtgac atctctgagt tcgcccgtat gctccgcggt caacaagggg
      421 tcacatccaa gatcaactcg aacaacatca ggcagatcgt ctcccaatga acactgaatt
      481 gttctccatt caatgccgat cggatactca cccactcttt ctccttccac aaaaacggca
      541 agagttggga acacctttga agccgagttc acggatgcgc tcagaaaggc gtgcggacat
      601 cgaacgtgta gttgaagaac tcgattcctc cctgcttcgg gtgaagactt gagaaaggcg
      661 gatgcactct tgctcatcca ccgcaccgcc tccgagtagt accccgactc agctggaaac
      721 caaccttctc caaggaagtc gtctgaatga cccatcatga ttcgagaggg tgccttatcc
      781 gatttctccc tcagcagctc gtagtcctgt cgcggaggaa agaaatgtgg cacctggttt
      841 gtcggaacgc acatcttgag aaaagaataa tgctgcctgt tccagcggtt cttgagacga
      901 tacaagatca gccccggaaa catcttcaga aattgccaca aagccttcag cagaacttcg
      961 gtgtttcgac tctcgcccaa tcgcaagagc ctcaatgctt ctctggcatc tcgtttgaat
     1021 tgtcttggaa agacggaaag agtttttgcc aaagggaaat ggcagaacat aaaacgccac
     1081 ttgttcacgg tgcaaagcgc cagcttccga ggcgattcat gaccaaagga cgcagaaaac
     1141 ttgtgataca cgatggcatc aggcacgtag acgagacgat atcccaatcc ccgaatgcga
     1201 attgaaagat cgacatcgtc atagtaggcg aagtactttg gatcgaagcc cttcaccttc
     1261 tccaaaactt cacgtcgtat cataaaagag catccgcaga tgctgacgat ctcctgctgt
     1321 ttcccccagc gattcgggtc aaaacgtctc ccgaaaccaa tgtcccaagt gtgtccccat
     1381 tggttcatct catgacccag actgttcaca atgtcccgct gccagtagct gagaatcctc
     1441 gagccacacg ctccaatgcc tggatcagac tgcatggctt ccacttgtct ggtcacccag
     1501 tcccgatcca cttcgacatc gttgttgaga gcaatcacat agtcggcacc ctgtgccaaa
     1561 acgtggtcaa acccaagatt gtttccccgc gcgaaaccaa gattctcctt gcagaggatt
     1621 gactctacct gaggatactt cttctgtgcg tactcaccac ttccgtccgg tgaggcgttg
     1681 tctaccatga gtatgtttgt gttgggataa ctctgcgcca gcaaagactc cagacagacg
     1741 tcaatgtgct tggcaccatt gtagttcaga acgactacat agacgctggg cagttgacct
     1801 ggatggatat ccgtggtcac gttggcctgc ggcgtagcag agccgttggc ctgttccaga
     1861 cttgagatga attcgcggta ccgactggcg gccaggtcaa gacgatgctc ctgaagaacg
     1921 tgccgtctcg tatttctgcc aatctcgtc
//
LOCUS       DAOQUC010000833         1949 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_75481_length_1949_cov_2.624076, whole genome shotgun sequence.
ACCESSION   DAOQUC010000833 DAOQUC010000000
VERSION     DAOQUC010000833.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1949
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75481_length_1949_cov_2.624076"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..328
                     /locus_tag="PKH07_13135"
     CDS             <1..328
                     /locus_tag="PKH07_13135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35928.1"
                     /translation="VLLVTNNYQLMRMVVSWPNLPRRHQPQGEGHGPSDDDTWRWLWD
                     NVSYDIKQWGLMSGIPENFTSEFREVLVGNRLIYPDGTVNKFIERYLREQVLGLFDKK
                     DTKGKR"
     gene            complement(409..789)
                     /locus_tag="PKH07_13140"
     CDS             complement(409..789)
                     /locus_tag="PKH07_13140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007291379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNT35929.1"
                     /translation="MRPILLDTDVLVDFFRGYGKAVAFVNTHSDRIVLSSIVVAELYA
                     GVKGDAERTALDDFVSLFRVLPVDAEIAKAGGLYRRDYGKSHGVGLGDAILAATAEAE
                     NAELVTLNTKHYPMLRNLKPAYNK"
     gene            complement(1686..>1949)
                     /locus_tag="PKH07_13145"
     CDS             complement(1686..>1949)
                     /locus_tag="PKH07_13145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35930.1"
                     /translation="SIWNGQQYRHLRKTVNNRRPPANCQHCPLRGIAYTSHHCNDADS
                     LLRQIGPTEDIDTSFFVRMKIKQFLDRSSLGRYILRIRDDKRR"
BASE COUNT          455 a          520 c          491 g          483 t
ORIGIN      
        1 cgttctactg gtcaccaaca actatcagct catgagaatg gtcgtctcct ggccaaacct
       61 gcctcgtcgt catcagcccc aaggggaagg tcatggtcca tctgacgacg acacttggcg
      121 gtggctctgg gacaatgtct cctacgacat aaagcaatgg ggtctgatga gcggtatccc
      181 agagaacttc acatccgagt tccgagaagt gctcgtcggt aaccggctga tataccccga
      241 tgggacggtc aacaagttca ttgaacgcta cctcagagag caagtcctcg gcttgttcga
      301 caagaaggac acaaaaggaa aaaggtgagg gaaccatccc acaccagtgc tggcctttct
      361 tgactgcgtc ttcggattgg tgggcatttg gcagaaccta aacgctccct acttattata
      421 cgcaggcttg aggttcctca acatagggta gtgcttggtg ttaagcgtta ctaactccgc
      481 attctcggct tcagcggtag cagccaggat cgcgtctcca agaccgacgc catgcgactt
      541 cccataatca cgcctgtaaa gccctccggc cttcgcgatc tccgcgtcta cagggaggac
      601 gcggaagagt gagacaaaat cgtcgagagc ggttcgttca gcgtctcctt tgaccccggc
      661 gtacaactct gcgacgacaa tggatgacaa aacgatccga tcagagtgcg tgttgacgaa
      721 cgccaccgcc ttaccatagc cccggaagaa atcaacaaga acatccgtgt ccaaaagtat
      781 cggtctcatt acggtcggca cctgtcccat tcgctgcgta tggcgccaaa atcaggaaga
      841 tcccgtcggt ccttccagat tccggcggat ttccgcaata ctgactcccg ccacccacgc
      901 ccagtttgat cgatgaggag atcgatcgcg tcacggatga gctcgctttg tctcttcctt
      961 tccgcccttc caatggcaac caacctatcg cgctgccttt cagtcaaata gacctgcgta
     1021 cgcaccatgt cgtgcctcct ggtaatgtat acagcagtag tatacattag aatcgtccgt
     1081 tgtcaagatc tactgaagtg gcagagtagg tctagcctcc ctatcaggga tattccaaaa
     1141 tcttcatgga aaatgaagtc caagccaggt gttttggcac tgtcaatagg cgagtacatt
     1201 tgttgccaat tctcctgcag ttgcgttaat acagcatgcc ttggatgtcc tcggatatcc
     1261 cggtctgacg agcatccaaa ggtgttccct gtctgagtcg tggctcgggc tagacggcga
     1321 caggataact acgcagccgt gccaaaactg tgccaaccaa gggtagttca ggggtggtct
     1381 gagggtggtg tcaacgtggg gtgctgaggc ttaaccattt gctaagacca tatttacaat
     1441 gcacttacga catgttcatt ttgtgcatgg ggtctagggg gtcgctggtt caaatccagt
     1501 cgccccgacc aacctaagac cttacccaga gagtgctgct gataattgca cctgcacaga
     1561 ttggtcttcg ccttctttcc gctgtcttga gttgctgtgt gtgcccttga ttttgagaag
     1621 gtggatccga caagagcaac actaccggaa tgctccagtc ctcagttggg taactcaaga
     1681 ggcaatcagc gtcgcttatc atcccttatt cgcagtatgt atctgcccag cgatgaccta
     1741 tcaaggaatt gtttgatctt catccgaaca aagaaagagg tgtcaatatc ttctgttggt
     1801 ccaatctgcc ttagaagact gtctgcgtca ttgcagtggt gactggtata cgcgattccc
     1861 cgaagtggac aatgctgaca gttggcaggt ggtctgcggt tgttgaccgt ctttctcaga
     1921 tgtctgtact gttgtccatt ccagatact
//
LOCUS       DAOQUC010000834         1945 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_75719_length_1945_cov_2.816402, whole genome shotgun sequence.
ACCESSION   DAOQUC010000834 DAOQUC010000000
VERSION     DAOQUC010000834.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1945)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1945)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1945
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75719_length_1945_cov_2.816402"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(46..1287)
                     /locus_tag="PKH07_13150"
     CDS             complement(46..1287)
                     /locus_tag="PKH07_13150"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase family protein"
                     /protein_id="HNT35931.1"
                     /translation="MGTSSYLAYDLGAESGRAVLGRLSQDNMTLEEIHRFPNGPVRVM
                     GSMYWDVLRLFSEMKLALRKCMDTPLDGIGVDTWGVDFGLLGRDDVLLGNPYHYRDAR
                     TDGMFEEAFRVVPRQTIFEHTGIQFMQLNTLYQLLAMVRSNSVFLERAETLLLMADLF
                     HFWFCGRKTAEFTLATTSQAYDPRKEAWSKFLLDSLQIPSHIFPEIVPPGTVLGDLLP
                     SLQSELGVGAIPVIAPASHDTGCAVAAVPAEGERWAYLSSGTWSLMGVEVTRPVINEK
                     SLAYNVTNEGGVQGTFRLLKNIAGLWLVQECRRVWERQGKTYSYDQLTELASHATHFK
                     ALLNPDDPRFLNPTDMPEVIRAYCRETGQAIPETPGEVVRCSLESLALKYKFTLRQIE
                     DLIGTDVERLHIVGGVRRTDF"
     gene            1571..>1945
                     /locus_tag="PKH07_13155"
     CDS             1571..>1945
                     /locus_tag="PKH07_13155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35932.1"
                     /translation="MKRIVLVALIAAFPFSVMAQKTEDFASKVTDVAVFKDGHALVMT
                     RAEALFDKGCCRTQNVPFPLIGTFWAFVNGAETEIQIVKSGFVEEQTSLPCMSFEDII
                     QANKGRSAVITESSRATHSGTLL"
BASE COUNT          479 a          527 c          477 g          462 t
ORIGIN      
        1 ggccggtaat caccgggatt cccagagcat ctgcggcgaa ctgattcaga agtctgttct
       61 gcgaacccca ccgacaatat gcagtcgttc aacgtctgtg ccaatcaagt cttcgatctg
      121 acgcagtgta aacttgtatt tcagtgcaag gctctccaac gaacaccgaa caacttcgcc
      181 cggggtctcc ggaatggctt gaccggtttc acgacagtaa gcacgaatga cttccggcat
      241 gtcagtcgga ttgagaaatc ttggatcgtc cggattgagc aatgccttaa agtgtgttgc
      301 gtgtgaagca agctcggtca gttgatcata gctgtaggtt ttgccttgac gctcccaaac
      361 ccggcggcat tcctgcacga gccagagacc cgcgatgttt ttcagaagcc ggaaggttcc
      421 ctgcacccca ccctcattgg ttacattgta cgcaagcgac ttttcattga tgacaggccg
      481 tgtcacttca acgcccatca gtgaccatgt tcctgaactc agatacgccc aacgttctcc
      541 ttctgctggc acggctgcaa cggcgcatcc cgtgtcatgc gatgcaggag caatcacggg
      601 gatggcaccc actcccaatt cggattgcag agacggcagg agatcaccca gcaccgttcc
      661 aggcggcaca atttcaggaa aaatgtgact cggaatctgg agactgtcca agaggaactt
      721 cgaccaagcc tcttttctgg gatcataggc ctgagacgtt gtcgccaggg tgaactcagc
      781 ggtctttcgt ccacagaacc agaaatgaaa gagatctgcc atcagcagaa gcgtttctgc
      841 cctttctaga aacacggagt tcgatcgcac catggccagt agttgataca gcgtgttcag
      901 ctgcatgaac tggatgcccg tatgctcaaa gatcgtctgc ctcggcacca cccgaaatgc
      961 ttcctcgaac attccgtccg tacgggcatc tcgatagtga tacggatttc ccaagagaac
     1021 gtcatcgcga cccagcagac caaaatccac accccacgtg tccacaccga tgccgtctag
     1081 tggcgtgtcc atgcactttc gaagcgccaa cttcatctct gagaaaagtc gcaacacatc
     1141 ccagtacata ctccccatga ccctgaccgg tccattcgga aagcgatgaa tttcctccag
     1201 agtcatgttg tcctgggaga gtctcccaag aacagctcga ccactttcag caccgagatc
     1261 gtaagctaga tagctggatg ttcccatcgg aagtatacct cctgagtact cactagaaag
     1321 tctagggaat tgttgcagtc tgtcacaggc aattcaagat gaaaaatcta tgaagggtac
     1381 tggcttagtt acggatgggg tggaggtatg cttcgcttgc agaggctctg cgccgctgcc
     1441 gaagcatatc ctggccgtat caccagcata actgagccga tatctctcga caccgaaatg
     1501 acttgcgaac tgattgccat aaggactatc atccagcagt aactcaccca tccgaaagga
     1561 gattttcgtc atgaagagaa ttgtgctcgt tgctctaatt gcagcctttc ctttcagtgt
     1621 catggcacag aagacggagg atttcgcgtc caaggtaact gatgtcgcag tttttaagga
     1681 tggtcacgca ttggttatga ctcgcgcaga ggcgcttttt gacaaaggct gctgtcggac
     1741 acagaacgtt ccttttcctt tgataggtac cttttgggca ttcgtcaatg gtgctgagac
     1801 cgaaattcag atagtaaaat caggatttgt cgaggagcag acttcccttc cctgtatgtc
     1861 atttgaggac attatccaag caaacaaggg caggtctgct gtcatcactg agtcgtcccg
     1921 ggcgacccac tctggaacat tgctc
//
LOCUS       DAOQUC010000835         1945 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_75735_length_1945_cov_2.258730, whole genome shotgun sequence.
ACCESSION   DAOQUC010000835 DAOQUC010000000
VERSION     DAOQUC010000835.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1945)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1945)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1945
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75735_length_1945_cov_2.258730"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..835
                     /locus_tag="PKH07_13160"
     CDS             <1..835
                     /locus_tag="PKH07_13160"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNT35933.1"
                     /translation="ILVWRYIPLIQGSIIAFQDYSVVKESKFVGLTNFANILFDKAWW
                     MAMWRTLHYMGIMVVLGFFPPIMLAILLQEVSKGKVIYRTLYYLPAVMTGVVVLFLWK
                     MMYDKSPEGLLNQVIGLVGIKPQRWIEDTRLAMVCCILPMVWAGLGPGCLIYLAALKS
                     IPNEMYEAADIDGASFVGKIQHIVVPSLKGIIMIQFINFFIKSAQNAGYILIMTFGGP
                     AEATNVAGFEIFKQAYVYLKFGTATAMSWTLASMLLGFTVIQMKRLSRMEFRAAASKE
                     G"
     gene            853..1269
                     /locus_tag="PKH07_13165"
     CDS             853..1269
                     /locus_tag="PKH07_13165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35934.1"
                     /translation="MPIIGTVGAKSPKIRILNSLIHLVLCLGAVSMVYPMMLMVSGSV
                     KSNLDKNEMDPIPKYFRDEVHLFRKYVEAKYNEKVDFARGAHRTNYFSFEDVEKPEFV
                     VAPFIEDWKSYLTANPPSTTQIEPGFASGAGIIPKN"
     gene            1312..>1945
                     /locus_tag="PKH07_13170"
     CDS             1312..>1945
                     /locus_tag="PKH07_13170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35935.1"
                     /translation="MEKLNADLGTYYNTWWEMVGSPEGLHLRTGSLVFSAMRKKFQEF
                     KKTLPLSDVYVYSLSGQFIEDVLKVKYAPRLKVLNEKLGTSFVSFSEINLTRECPPEP
                     MRTPWLHYVRNSVNLQYVKVRKAETENYQRFLSERYSGSIEQLNDVYGTKHAAFSAMK
                     LPTEMPTAGASYADWEFYIVNRVAPENIYLEAAEFGFRDYLREKYATIEVL"
BASE COUNT          475 a          489 c          510 g          471 t
ORIGIN      
        1 gattctagta tggcgctata tccccctgat tcaaggatcc attatcgctt ttcaggatta
       61 ctcggtggtc aaggaatcaa agtttgtcgg tctgacaaac ttcgccaaca ttttgtttga
      121 caaagcctgg tggatggcaa tgtggcgaac tctgcactac atgggaatca tggtcgtgct
      181 gggattcttt cccccgatta tgcttgccat ccttctgcag gaagtctcca aaggcaaggt
      241 tatctaccga acgttgtact atcttcccgc cgtcatgact ggagttgtgg tgctctttct
      301 ctggaagatg atgtacgaca aatcccctga ggggttgctc aaccaggtca tcggtttggt
      361 cggaatcaag ccgcagcgat ggatcgagga tacgcgactt gccatggtgt gctgtattct
      421 cccaatggtc tgggcaggct tgggccccgg gtgcctgatc tacctggccg cgctcaagag
      481 cattcccaac gagatgtacg aagccgctga cattgatgga gccagtttcg tggggaagat
      541 acaacacatc gtagtcccca gcctaaaggg gataatcatg atccagttta tcaacttctt
      601 catcaagtcg gcacagaacg ccggatacat cctcatcatg acctttggag gtccggctga
      661 agccaccaac gtcgcaggct ttgagatttt caaacaggcc tatgtctatc tgaagttcgg
      721 caccgcaacc gccatgtcct ggacattggc gtccatgctg ctgggtttca cggtcattca
      781 gatgaagagg ctgtcccgaa tggaatttcg agcagccgca tccaaagaag gatagtagag
      841 aacgagcatc agatgccgat tatcggaact gttggagcca agtcacccaa gataaggatc
      901 ttgaattcct tgatccatct cgtgctgtgc ctgggtgctg tcagcatggt ctatccgatg
      961 atgctgatgg tgtccggctc ggtaaagagc aatctggata agaacgaaat ggatcctatt
     1021 ccgaagtatt tcagggatga ggttcatctg tttcggaaat atgtcgaggc aaaatacaac
     1081 gaaaaagtgg atttcgcccg tggtgcccat cgaacaaact acttctcgtt tgaggatgtg
     1141 gagaagccgg agtttgtggt agcaccgttt atcgaagact ggaaatctta cctgacggcc
     1201 aatcccccgt ccacaacgca gatcgaaccg gggtttgcgt cgggtgctgg gatcattccg
     1261 aagaactagc ggcttttcaa gaatgatctc cgccgacaat atgacggcaa cttggaaaaa
     1321 ctgaatgccg atctcggcac ttactacaac acgtggtggg agatggtcgg ctctccggag
     1381 ggtcttcacc tgcgcacagg cagtttggtc ttctcagcga tgcggaagaa gtttcaggaa
     1441 ttcaagaaga ccttgccact ctcggatgta tatgtctatt cactcagcgg acagttcatc
     1501 gaagacgtgc tcaaggtcaa gtatgcgcct cgactgaaag tgctcaacga gaaactaggc
     1561 acttcgtttg tctctttcag tgagatcaac ctgacacgag aatgcccgcc tgagccgatg
     1621 cgaaccccat ggctgcacta tgtccgaaat tcggtgaatc ttcagtacgt gaaggtgcgc
     1681 aaggccgaga cggaaaacta ccagcgcttc ctctccgaac gctattccgg cagcatcgag
     1741 cagctcaatg atgtctatgg aaccaagcat gccgctttca gcgccatgaa gctgccaacc
     1801 gaaatgccaa cggccggtgc ttcatacgcg gactgggagt tctacattgt caaccgagtt
     1861 gctcccgaga atatctatct cgaggcggca gagttcggtt tccgagacta tctgcgcgag
     1921 aaatacgcga ccatcgaagt gctca
//
LOCUS       DAOQUC010000836         1944 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_75763_length_1944_cov_3.462149, whole genome shotgun sequence.
ACCESSION   DAOQUC010000836 DAOQUC010000000
VERSION     DAOQUC010000836.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1944
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75763_length_1944_cov_3.462149"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1413
                     /locus_tag="PKH07_13175"
     CDS             <1..1413
                     /locus_tag="PKH07_13175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35936.1"
                     /translation="HAMDAAESELTFGKPAFKIALMSSLFVERNRFEEAEAARRVIES
                     NLRARGLTAQRLFYIAERALHHFQPGGKLFPGLDEPTLFLEETLPLLPAPSRQVMPVE
                     DSVWIGTHARDGRDIHFSFTKGLDPAVPPPPHATTLILGEPGAGKTSLLRWIMLQRLL
                     QGRTVISIDPEGENNKLCEAVGGKVVPAGIPEDTETCLIHPLQGENPAELLLAVCFLI
                     ASLAGESVLSPAVQAALHEAVKRRWERRPGAMTIAEFVETLGTINVPEAAMPLALLRP
                     FMRGGLFEGFFDRPKALLSPHFPAGQWWNFDLSSLREENRAIVHSVLAWFLYHAVTVG
                     KQPMDIFIDEGWRLLRSGPFTDLLDELGRRARKRGVGVTLITHLPADLMKNPTSLSLA
                     STAFIGRLGPDEAFAFYRSLGVPESEARKNAETTSRLPPRVFLAAPSAGRGALFPVLV
                     SIPPTWIQFWKQLGATGVAR"
     gene            1410..>1944
                     /locus_tag="PKH07_13180"
     CDS             1410..>1944
                     /locus_tag="PKH07_13180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35937.1"
                     /translation="MIYPKSGYMCIHCGYEHATAPDQCPLCNGEWQGWELLFAPHALD
                     DLRAQVMGWIAQLPFPTIMEWIASPKGLRVRLYLHPHVAEGVVQSWSAMTGQHSRWRS
                     LGIVKVQSDGYALHTSNRLPSLIASAKDSDPLLAIGSRLISGAREGQDASLRLWLLGN
                     EDQLQEKLRSLSSYSYGT"
BASE COUNT          401 a          549 c          526 g          468 t
ORIGIN      
        1 cacgcaatgg atgctgccga gtcggaactg acctttggta aacccgcctt caagatcgcc
       61 ctaatgagtt cgctctttgt cgagcgtaat cgctttgaag aagcggaagc cgcacgcaga
      121 gtgatcgagt ccaatcttcg cgcgcgtggg ctcacagccc aaagactctt ctatattgcc
      181 gaacgtgcct tgcatcactt ccaacccgga ggaaagttat ttcccggatt ggatgagccg
      241 acgctgttcc tggaagaaac acttccatta ttgcctgccc cttcccggca ggtcatgccg
      301 gtcgaggatt cggtctggat cggcacgcat gccagggatg gacgggacat ccacttctcg
      361 ttcaccaagg ggcttgatcc ggcagttccg cctccacctc atgccacaac actcatcctc
      421 ggtgaacctg gtgctggtaa gaccagcctg ttgcgctgga tcatgttgca acgtttatta
      481 caaggcagga cagtcatctc catcgatccc gaaggcgaga acaacaaact ctgtgaagct
      541 gtcggcggaa aggttgtacc tgctggcatt cccgaagaca cagaaacctg cctcattcat
      601 cccctgcaag gcgagaaccc ggcagagtta ttactggctg tctgtttcct aatcgcctcc
      661 ctcgccggtg agtcggtcct atcgccagct gtgcaagctg ccctgcatga agcggtaaag
      721 agacgttggg agcgcaggcc gggcgcaatg accattgcgg aattcgtgga aacattggga
      781 accatcaatg tcccggaagc tgcgatgcct ttggcgctgc tgagaccctt catgcgcggc
      841 ggactcttcg aagggttctt cgatagaccc aaagctctat tatctccgca cttccccgcc
      901 ggtcagtggt ggaatttcga tctttcttca ttgcgtgaag aaaaccgtgc cattgtgcat
      961 tcggtgttgg catggttttt gtatcatgcc gtcacagtcg gtaaacaacc gatggacatc
     1021 ttcatcgacg aaggctggcg cttgttgcgc agcggtccct tcaccgatct gctggatgaa
     1081 ctgggcagac gcgcccgcaa acgtggtgtc ggcgtgactc tcatcacgca tctgcctgcc
     1141 gatctgatga agaaccccac ttcgttgagt ctggcatcca ctgcctttat cggtcggctc
     1201 ggaccggatg aagcctttgc tttctatcgc tcgctgggcg ttccggaaag cgaagcacgc
     1261 aagaatgctg agactacttc acgtttgcct ccgcgagtct ttcttgctgc tccatctgcg
     1321 ggacgtggtg ctttgtttcc cgtcttggtc tccatcccgc cgacatggat ccagttctgg
     1381 aaacagctag gggcaacagg agtggcacga tgatttaccc aaagagcggg tacatgtgta
     1441 ttcactgcgg ctatgaacat gctactgctc cagatcaatg tcccctctgt aatggtgaat
     1501 ggcagggatg ggaactgctc tttgcgccac acgcattgga tgacttgcgc gcacaggtca
     1561 tgggttggat cgcgcaactg ccatttccca cgatcatgga atggatcgcc tctcccaaag
     1621 gcttacgtgt gcgcttgtat ctgcatccgc atgtggcaga aggagtcgtg cagtcttggt
     1681 ctgccatgac cggacaacat tccaggtggc gcagtctggg catcgtgaag gttcaatccg
     1741 atggatatgc ccttcataca tcgaatcgtc tgccatcgct cattgcctct gccaaggata
     1801 gcgatcccct gcttgccatt ggcagccggt tgatcagcgg cgctcgcgaa gggcaggatg
     1861 ccagtctacg tttgtggttg cttggcaacg aagatcaact gcaggaaaaa ttgcgcagtc
     1921 tgtcttccta ctcgtatggc accg
//
LOCUS       DAOQUC010000837         1943 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_75823_length_1943_cov_3.447034, whole genome shotgun sequence.
ACCESSION   DAOQUC010000837 DAOQUC010000000
VERSION     DAOQUC010000837.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1943)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1943)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1943
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75823_length_1943_cov_3.447034"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..961
                     /locus_tag="PKH07_13185"
     CDS             <1..961
                     /locus_tag="PKH07_13185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35938.1"
                     /translation="LAFGQLTDATDTPCLVVAAKAADVGSLRRAGTVLGINARPASAT
                     MTLEQRWIDVNKDKLAFKITGARGYGEAGEVIRAVSLDDDATIDSLIIGSSNVDPNLA
                     ATLSDHWGATYILYTPIPAGAEFALNPLGAPSPVPTLGYKKFKGVGGYDVAVARVAVG
                     AATETLYAISNSVASSTPENEQYAGRVYLFYGSSIAGVTDVDISKKQADVVISGAFKN
                     DEMGRSILFGPVGVGGNLALILGATFMDPKIPSETDPRYDAGQMYLFPAAMFSPIPGD
                     SNADQYLNFKDVFYQSLLWSGNMDWRILDNWQGRNAFPSPRAQ"
     gene            1063..>1943
                     /locus_tag="PKH07_13190"
     CDS             1063..>1943
                     /locus_tag="PKH07_13190"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNT35939.1"
                     /translation="MNQSSSCVYVVSLGCPKNLVDTERVMADLVRSGFSITTDLDQAR
                     IVIINTCGFIRSAEQESLETIEEFCEMKRHGDIDILVVWGCLVSRRRDACRQLDPSGL
                     VDAWLPVAEKGAVAEVIRRKTATLVVPNDPQPTHHRILATPPHTAYLRIADGCDNRCS
                     YCTIPSIRGSFHSRPIEEIIAEAQTLADAGTVELNLIAQDSTRYGLDLYGEPRLDGLL
                     HQLRTIEPLRWIRVLYAHPALVTEALVKTLASTPQVCRYLDIPIQHASDSILAAMKRR
                     VTRKQPEQMVALLRREIP"
BASE COUNT          414 a          538 c          534 g          457 t
ORIGIN      
        1 acttgctttt ggccaactga ccgacgcaac agataccccg tgccttgttg ttgctgccaa
       61 ggccgccgac gttggcagtc tgcggcgagc gggaaccgtg ttgggcatca atgcgagacc
      121 tgcctctgcc acaatgaccc tcgagcagcg ttggatagac gtcaacaagg ataaactggc
      181 cttcaagatc accggagcac gaggttacgg tgaagccgga gaagtgattc gtgctgtcag
      241 cttggatgat gatgccacca ttgactccct gatcatcggt tcatcgaacg tggatccgaa
      301 tcttgctgcc acactttcgg atcactgggg cgcaacctat atactctata ctccaatccc
      361 tgcgggagcg gagtttgctc tgaaccctct cggtgcacca agtccggtgc caactctggg
      421 ctacaagaag ttcaaaggtg ttgggggcta tgatgttgcc gttgcccgcg ttgcggttgg
      481 cgcggcaact gagaccctgt acgccatctc aaactctgtt gccagttcca cgccggaaaa
      541 cgaacagtat gccggtcggg tctatctgtt ctatgggtca tccatcgcgg gagtcaccga
      601 tgtggacatc tccaagaagc aggcggatgt ggtcatctct ggagcattca agaacgacga
      661 aatggggcgt tccattcttt tcggacccgt gggggttggc ggaaacttgg ctttgatcct
      721 cggtgcgaca ttcatggatc cgaagattcc ctcagagacg gacccacgat atgatgcggg
      781 acagatgtac ttgttccccg cggcgatgtt cagtccgatt ccgggtgata gcaatgcgga
      841 tcaatacctg aacttcaagg atgtctttta tcagagcctt ctctggtcag ggaatatgga
      901 ctggcggatt ctcgacaact ggcagggtcg taacgcattt ccaagtccca gagcgcagta
      961 gtggagaacg ccgaaaggcg gacactccct ttgaccctac tggtctcttg tttccattga
     1021 acgccgtgaa gacggaagac cctcatccat tttgggagcg tgatgaatca atcctcgtcc
     1081 tgcgtctatg tggtttcgct cggctgtccc aagaatctcg ttgacaccga acgtgtgatg
     1141 gcggatttgg tgcgttcggg tttctcgatc actacagatc tcgaccaggc gcggattgtg
     1201 atcatcaaca cctgtgggtt catccgctca gcggagcagg aatctctaga gaccatcgaa
     1261 gaattctgcg agatgaagcg ccatggggat atagatattc ttgtggtgtg gggatgtctc
     1321 gtctcgcgtc gtcgagacgc ctgtcgccaa ctagatccat ccgggctcgt ggatgcctgg
     1381 ttacccgttg ccgaaaaggg tgccgttgcc gaggtcatcc gcaggaaaac ggccacactt
     1441 gttgttccaa atgaccctca accaacgcac catcgaattc tggcaactcc accccatacc
     1501 gcctatctga gaattgctga tggctgcgac aaccgatgtt cctactgcac catcccttcc
     1561 attcggggtt cctttcacag ccgacccatc gaggagatca ttgccgaggc gcagactctt
     1621 gcggacgcgg gtacggtcga actgaatctc atcgcccagg actccactcg ctacggtctc
     1681 gatttgtatg gagagccgag gcttgacggt ctgctgcatc agctgagaac gattgaaccg
     1741 cttcgctgga ttcgggttct ctatgcgcat cctgcattgg tcacggaggc gcttgtcaaa
     1801 acacttgcgt caacacccca agtgtgtcgc tatctggaca ttcccattca gcacgcttcg
     1861 gattccattc tcgcggccat gaaacggcgt gtgacgcgca aacaaccgga acagatggtg
     1921 gcactcctgc gtcgggaaat ccc
//
LOCUS       DAOQUC010000838         1943 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_75844_length_1943_cov_2.845339, whole genome shotgun sequence.
ACCESSION   DAOQUC010000838 DAOQUC010000000
VERSION     DAOQUC010000838.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1943)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1943)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1943
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75844_length_1943_cov_2.845339"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1943)
                     /locus_tag="PKH07_13195"
     CDS             complement(<1..>1943)
                     /locus_tag="PKH07_13195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35940.1"
                     /translation="HILFQSLRLDGDCTVSYLNCRDKGRVWTPIKDLSRHFSMGVGTC
                     LLSHQGVLHAAWCPTERGLNGLFYRQSADGGTTWSTRIAVTETTKAIYFPKLFADGSL
                     LYLGWVEQQIIGKDLSLTSPDVQQRDVQHLEEAERRLSTNVSNDKYDSTIRMSMSRNN
                     GRTWESNIPVKRVFLRISNYRFFVSGTKQEFAYKTPEGCVLLTSSDSGLTWKEETITE
                     QQFGNPQPPTIVEYRGDTFQVGTHKVNTRDVLYIRRTDSTPPEAKMLTAEQVIDATQI
                     VVTWAGTDDWSEDANLVFSSRLNDASWSAFESTTERSLSDLADGEHTFSVMARDEAGN
                     VSVQSDSVVFTVMCPPDTILVATPPPMSNDLRPRVKWTGVDNTTSPNRLLFQYRLNSE
                     EWCDPRPVQELALSSLTNGDHTFEVRAIDEIGAVDPSPALCSFRVDTLPPQTTRAEVR
                     PYVRGATSLFVDIAATDNYSATSELRFSLKLDDQEWTPFILFPTIPVDGLKDGLHQLR
                     VRSKDQAGNVEDPPRIVPFEVSIPPKVEISDAPDKPIVPGLFELFLSIWDNETPEEEV
                     RLRFRINGGLWSGVVHGPILQVNASRLGDGRHQLEIEATDSSDLKDPVTTMYEFVVDS
                     NQIPPPARLEGIRYGSKVDLSWP"
BASE COUNT          444 a          519 c          519 g          461 t
ORIGIN      
        1 tccggccaac tcaaatcaac cttgcttcca tatcggatgc cttccagacg cgctggtgga
       61 ggaatctgat tgctgtccac cacaaactcg tacatggtcg ttacgggatc cttcagatcg
      121 gaggaatccg ttgcctcgat ttcaagttgg tgtcttccgt ctccaagtcg cgaagcgttc
      181 acctgaagaa ttgggccgtg aactacaccg gaccaaagcc caccgttgat tcgaaaacga
      241 agtctgacct cttcttccgg cgtctcgtta tcccaaatgg agaggaatag ttcgaacagt
      301 cccggaacaa tgggcttatc cggcgcatcg ctgatctcga ccttgggcgg aatgcttact
      361 tcaaagggaa cgattcgcgg gggatcctca acattgcctg cctgatcctt ggaacgcacc
      421 cgcagctgat gcagaccgtc tttgagtcca tccaccggaa tggttgggaa caagataaac
      481 ggagtccact cctgatcgtc cagtttcagg gagaatctca gttccgaggt agcggagtaa
      541 ttatcggtag cggcaatatc cacaaaaagg ctggtcgccc cgcggacata aggccgcact
      601 tctgcccgag tggtttgtgg tggcagggtg tccactcgga aagaacacaa cgcgggtgat
      661 ggatccactg ccccaatctc atctatcgca cgcacttcaa acgtatggtc tccattggtt
      721 aatgaagaca gagccaattc ctgaacgggc cgagggtcgc accactcttc cgaattgagg
      781 cggtactgaa agagaaggcg gttgggtgag gttgtgttgt ccacacccgt ccacttgact
      841 ctcggtcgga gatcattgct cattggtggc ggagtggcga ccaggattgt gtctggtgga
      901 cacatcaccg taaacactac ggaatctgac tgaacgctta catttccagc ctcgtcgcgg
      961 gccatgacgc tgaaggtatg ctctccatcg gcaagatcac tgaggcttct ctctgttgtg
     1021 gactcgaatg cagaccagga tgcatcgttc aggcgggaag agaacaccag gttggcatcc
     1081 tcagaccaat catcagtgcc tgcccatgta accactatct gcgtggcatc aatcacctgt
     1141 tccgccgtaa gcatctttgc ttcgggagga gtggaatcgg ttcgtctgat ataaagcaca
     1201 tctcttgtgt tgactttgtg agtaccgacc tggaaagtgt ccccgcgata ttcgacgatg
     1261 gttggtggct ggggatttcc aaattgttgt tcggtaatcg tttcttcctt ccacgtcagg
     1321 ccggaatcag aactggtcag caaaacacaa ccttcgggcg tcttgtaggc aaactcttgc
     1381 ttcgtgccgc tgacaaagaa gcgatagttg ctgatgcgca gaaagacccg cttgacaggg
     1441 atattggatt cccacgtacg gccattgttg cgactcatgg acattcgaat cgtggagtca
     1501 tacttgtcat tgctcacatt ggtagaaagc cgcctttcgg cttcctcaag gtgttgcaca
     1561 tcgcgttgct gaacgtcggg agacgtcagg gaaagatctt ttccgataat ctgttgctcg
     1621 acccatccca gataaagaag gctgccatcc gcaaagagct tcgggaaata aatcgctttg
     1681 gtcgtctcgg tcactgcgat tcgggtagac catgtcgttc ccccgtcggc gctctgtcgg
     1741 tagaacaaac cgttcaggcc tcgctcggtg ggacaccacg cagcgtggag cactccctga
     1801 tgggacaaaa ggcacgttcc cactcccatg ctgaagtgcc tggaaagatc cttgatcggt
     1861 gtccaaaccc ggcctttgtc ccgacagttc agatagctga cagtacagtc tccatccagt
     1921 cgaagggact ggaacaggat gtg
//
LOCUS       DAOQUC010000839         1942 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_75890_length_1942_cov_3.772125, whole genome shotgun sequence.
ACCESSION   DAOQUC010000839 DAOQUC010000000
VERSION     DAOQUC010000839.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1942)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1942)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1942
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75890_length_1942_cov_3.772125"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(133..495)
                     /locus_tag="PKH07_13200"
     CDS             complement(133..495)
                     /locus_tag="PKH07_13200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35941.1"
                     /translation="MVKHRYNLIPWWRSALIPVGHYGMESGAHKSGITEIPMIGDVSR
                     HGLTITTPTRFGEGKTMLAEEGSGLLSSRSSDISLADFGTFGNSRDPNVGRLKPLFDR
                     SAMRLSSSGGKDRSNEGY"
     gene            857..>1942
                     /locus_tag="PKH07_13205"
     CDS             857..>1942
                     /locus_tag="PKH07_13205"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HNT35942.1"
                     /translation="MQAKCASTTLVALALLSVLSVPCWSSPVAFNSAEGGTYMVQREG
                     AATAEAAVLALLQGPAKMESAAGIWTAIPKGTKLLSVDILDKGVLVNLSAEAAVGLDD
                     ATWETMQQQFIWTMKPLGLEEMVKVLIDGKEPTSYMPEPGPALYSAPSDDEVSTSAIE
                     LGDVGLSGKRVTVSAGHGHYWNGSAWYYERGTSCGYEQEDIRNVRFGKYLRAHLENDG
                     AYVQIVREDNLNRGTGPSNVPWFHEGSYIWCRDQSYSCSVWANATGRCTDIVGPTRLS
                     DSIRARPLASNLDDRGNTDVFIDIHSNAYQGDCYGTSCPSGPDGYYCNDNGHQTWGAQ
                     SISLAQKVQDQMLAAVQAAGYSMGHHGG"
BASE COUNT          450 a          471 c          545 g          476 t
ORIGIN      
        1 attgacaaag cgcctggaat ctcttccttt tgacgctttc gcaccattct ctaacgaaga
       61 ctgtctgatt tgtcttttcc tgatgagcga atggtgcgaa agcgtcaaaa ggaagagatt
      121 ccaggcgctt tgtcaatagc cctcgttgga gcgatccttt ccgcctgagg aactcagacg
      181 catggcggag cggtcgaaga ggggcttgag acggccgacg ttgggatctc tggaattccc
      241 aaaggtgccg aagtccgcca agctgatatc gcttgatcgg gaggaaagca ggccgctccc
      301 ctcttcagcg agcattgtct tgccttcacc aaaacgggtt ggggtcgtta tcgtcagccc
      361 gtgacgggat acgtcgccaa tcatgggaat ctcggtgatc ccgctcttgt gcgctccact
      421 ttccatcccg tagtgcccca caggaatcaa ggcacttctc caccagggga tcagattgta
      481 cctgtgcttg accatgactt cttcgttttt tgcttgacaa aagcgttttc agaatggcaa
      541 actatgaaca gaatcgttat gtaaagattc tacgtaaaga gtttgatgcg ttgttttgtc
      601 atgagtacct gactgcgatg tgatgtaatc tgccctgaaa gggggtgcat ttgcagattc
      661 tgcgggcttt cttcagtgag attcgatagt tgcgctcttc ttatgttgtt caggatgttg
      721 ggaattgttc cttttcggag tgcctgattc tctcgtcttt gagaaaacaa acatcttgtc
      781 gccttgagaa gaagagcaag gggagtaagc ggttactaat tgagttttgt atcataagaa
      841 aggataggct tttgaaatgc aagcaaaatg tgcttcaacg acactagtgg cattggcact
      901 gttgtctgtt ctttctgttc cgtgttggtc gagtccggtt gcgttcaaca gcgcagaagg
      961 cggcacctac atggtacaga gagaaggagc agcaactgcc gaggctgcgg ttctggccct
     1021 gctgcagggg ccggcaaaga tggagagtgc tgcgggaatc tggaccgcga ttcccaaggg
     1081 aacgaaacta ttaagcgttg acatcctgga caagggtgtc ctcgtcaacc tgtctgctga
     1141 ggcggccgtc ggcttggacg acgccacatg ggagaccatg cagcagcagt tcatctggac
     1201 gatgaaaccc ttgggcttgg aagaaatggt caaagtgctc attgatggaa aagagccaac
     1261 cagttatatg cccgagcctg ggcctgctct gtactctgct ccatcagatg atgaggtcag
     1321 cacatcagcc attgaattgg gcgacgtagg cctttcggga aaacgtgtta cggtttcagc
     1381 aggacatggt cactactgga atggcagcgc ctggtactac gaacggggaa ccagttgcgg
     1441 ttacgaacag gaagacattc gcaacgtccg tttcggcaag tatctgcggg cacatctgga
     1501 aaacgacggt gcctacgttc agattgttcg tgaggacaat ctaaaccgag gtaccggacc
     1561 cagcaacgtg ccatggttcc acgaaggttc ctacatctgg tgtcgagacc aaagttattc
     1621 atgcagtgtc tgggcgaatg ccactgggcg atgcacggac atcgtcggtc caacgcgctt
     1681 gagcgactcc attcgcgcaa gacctttggc ttccaacctg gatgatcgag gcaacacgga
     1741 tgtcttcatt gacattcact ccaacgccta tcaaggcgac tgctacggca cgagttgtcc
     1801 atcgggtcct gacggctact attgcaatga caacgggcat cagacatggg gtgcgcagag
     1861 catcagtctc gcacagaagg tgcaggatca gatgcttgcc gctgttcaag ctgctggtta
     1921 ctccatgggg caccatggcg gc
//
LOCUS       DAOQUC010000840         1941 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_75976_length_1941_cov_3.124072, whole genome shotgun sequence.
ACCESSION   DAOQUC010000840 DAOQUC010000000
VERSION     DAOQUC010000840.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1941
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75976_length_1941_cov_3.124072"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            236..>1941
                     /locus_tag="PKH07_13210"
     CDS             236..>1941
                     /locus_tag="PKH07_13210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35943.1"
                     /translation="MKTLFLIVVVGLFLCVSMFASAEQVETSVAQVQTSLRGVDGVVD
                     AYGGLHLAWIDGGTLQYATSYDDGKSWYQYSINTPRNGTYATRMGGLHLAIDPWGNAF
                     MFTNVYFGNAGYGQEFFYKIYCITNVTGVWQEEVVVDYLTYFYRYDSAGDFYLNNLAN
                     PFDAFSDAQGNIHLFLRHFGWWSYGGELFEKVRSAGSTGAWGGLTSICHIYHPNVDFQ
                     SSIYEQAIQTSDGGAVVIALNRDGNTNTSLPVQNQNRFVYYLERAAGGAWSNYQPFSI
                     GDNYEGSKGFMNIDVDEGDNIHVLHVQPGFANLLYTRNWGTPELVATVPTANDRFNTC
                     LLDAQADGLLTVGYTLYNTATSSYTRYPSLIERDGAGPWSPPVAFSDRVQTMTPVTGV
                     ARFDTYLGGSYEGPLRWLCWGPAAYPLSLVYLQDEPRKPEPDFEIKLVDPVTLYTAGE
                     QPQFRVTVLPKNGFNQEVNLFLHSEYPPARLTADTVLPPAVTTVLGEQIPEVGESTEY
                     HMFLTADSSDGRKFRDLFIPYQIFYPIDEMSALSLNSGRETIRVGETLPLSGRLLPGL
                     EQ"
BASE COUNT          436 a          467 c          528 g          510 t
ORIGIN      
        1 aaaaaattta gctcattgcg tgaggggact gaactggttc agtcccctcg ttcatttgtg
       61 tctctgtgag ttgatcttgc atcccaggat gagagctgtt ctgttgtctg aatccgtacc
      121 tatttgccat cctctgttga ccattgtgag gatgagttct agtatcaggc aatggggaag
      181 cgattgatca gcgcatcggt tcgacgatgc agcgatacaa aggaggatcc ttcccatgaa
      241 gaccttattc ctgatagttg tcgttggtct gtttctctgt gtcagtatgt tcgcatctgc
      301 agaacaggtg gagacttctg tggcacaggt gcagacatct ttacgcgggg tggatggtgt
      361 cgtagacgcc tatggcggtt tgcatctggc ttggatagat ggtggaacac tgcaatatgc
      421 cacttcctac gacgatggaa agtcctggta ccaatacagt atcaacacac cacgaaatgg
      481 aacctatgcc actcggatgg ggggattgca tctggcgatt gatccttggg gcaatgcttt
      541 catgtttaca aacgtctatt ttggcaacgc gggctatggc caggagttct tctataagat
      601 ctattgcatc accaacgtca cgggagtctg gcaggaagag gtcgttgttg actatctgac
      661 gtatttctat cggtatgatt cggctggtga cttctatctg aacaatctcg ccaatccttt
      721 tgatgctttc tccgatgctc aagggaatat ccatcttttc ctgagacatt tcgggtggtg
      781 gagttacgga ggcgagttat ttgaaaaggt tcgctcggca ggatcaacgg gtgcatgggg
      841 tggcctgacc agcatttgtc atatctatca tcccaacgta gacttccagt ccagcatcta
      901 cgagcaggca atccagactt cggatggagg agccgtggta atcgccctca accgagacgg
      961 aaacaccaat accagtttgc cggttcagaa tcaaaatcgc tttgtctact acttggagcg
     1021 ggctgctggt ggggcctggt cgaactatca accattcagc attggggata actatgaggg
     1081 ctccaagggt ttcatgaata tagatgtgga tgaaggcgac aatattcacg ttctccatgt
     1141 ccagccggga tttgccaatc ttctgtacac tcgaaattgg ggcaccccgg aattggtggc
     1201 aacagtcccg actgccaatg atcggttcaa tacctgtctt ttggacgcac aggccgatgg
     1261 acttctgact gtgggctaca cgctctacaa cactgcgacc agttcctaca cacgctatcc
     1321 gagtttgatt gagcgggatg gcgctggacc atggagtcct ccagtggcct tttcagatcg
     1381 cgtgcagacg atgacgccgg tgacaggcgt ggcgcggttt gatacctacc ttggtggttc
     1441 ctatgaaggc ccgctgagat ggttgtgttg gggtcctgca gcctacccgt tatcattggt
     1501 ctacctgcag gatgaacctc ggaagccgga accggatttt gagattaagc tggtagatcc
     1561 ggttactctt tacacagcgg gcgaacagcc ccagttccga gttaccgtac tgccgaaaaa
     1621 cggattcaac caggaagtca atcttttcct gcacagcgag tatccacccg caagactgac
     1681 tgctgatacg gtgttgcctc cggcggtcac cacggttctg ggggaacaga tccccgaagt
     1741 aggggagtca acggaatacc acatgttttt gacggcggac agcagtgacg gcaggaagtt
     1801 ccgagatctc ttcattccct accagatttt ctatcccatt gatgagatgt ccgctttgtc
     1861 tctgaactcg ggtcgcgaaa cgattcgagt gggagagacg ctgcctcttt ccgggaggct
     1921 tttgcctgga ctggaacaac a
//
LOCUS       DAOQUC010000841         1938 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_76190_length_1938_cov_2.184811, whole genome shotgun sequence.
ACCESSION   DAOQUC010000841 DAOQUC010000000
VERSION     DAOQUC010000841.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1938)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1938)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1938
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76190_length_1938_cov_2.184811"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..558)
                     /locus_tag="PKH07_13215"
     CDS             complement(<1..558)
                     /locus_tag="PKH07_13215"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNT35944.1"
                     /translation="MKPRTLRFLLNKRSQLLFALVLLLPAYFLVVHALQTSAQQQDSA
                     QTQEAKPERFPPVADANIVLLMSDALRADHLGCYGYSRNTSPNIDKLAAEGVVLDRFY
                     SNACWTRPSMGTLFTSKYPGQLGINADNSFLPEGNITLAEALKLNKYTNVGVVTNGHI
                     WPEWGFGRGFDEYIKLQKWQYADETK"
     gene            complement(710..>1938)
                     /locus_tag="PKH07_13220"
     CDS             complement(710..>1938)
                     /locus_tag="PKH07_13220"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HNT35945.1"
                     /translation="TPNIDRLSLGGVLFRDAATNASYTKASVASLLTSTFPWVHGVLT
                     QLDILSDSLLTLPELLKESGYRTATIIQNSQASPVFGYGQGFDTVVEWYAPDFDALHK
                     SSSDGNPGFWTDLCKPTLEGMKDQPFFAYIHLIDPHTPYTPPKEFAQMYAPNYQGRIG
                     MGKDIMDMVQSGLMRFSPEDLAYFSSMYKAEVTSVDRFVGWLSDWLQENNLTHNTLVV
                     FLSDHGEAFMEHGDIEHGRSLYQELVHVPLVFSLPGVLPEGTDSGYPAQLLDVAPTVL
                     DFVGISAPEQMQGKSLLPMILGSHESDKGRARLGAVRNLIHFVRKGDMKLVRKLSTPE
                     SAQVKWYEYELFDLSVDPGEKINLWPSKPIEGRILQQLLTKSLSSEATVSGTKIEPEK
                     LDPAVLQNLKDLGYLR"
BASE COUNT          483 a          479 c          507 g          469 t
ORIGIN      
        1 ctttgtctcg tcagcatatt gccatttctg aagtttgata tactcatcaa aaccccgccc
       61 gaagccccat tccggccaaa tgtgaccatt cgtgacaact ccgacatttg tgtatttgtt
      121 gagtttgagc gcttctgcga gagtgatgtt gccttcgggt agaaaagagt tgtcggcatt
      181 gatgcccagc tgtcctgggt atttagacgt gaagagggta cccatggaag gtcttgtcca
      241 acatgcgttg ctgtagaaac ggtctaacac aaccccttca gccgccaact tgtctatgtt
      301 tgggctggtg ttacgggagt atccgtagca accgaggtga tcagcacgaa gcgcgtctga
      361 catgagcaga acgatatttg catcagcaac gggtggaaat ctttccggct ttgcctcctg
      421 tgtttgtgcg gaatcctgct gttgcgctga tgtctgaaga gcgtgcacga ccaaaaagta
      481 agcaggcaac agcaaaacaa gagcaaagag aagctggctt cgcttgttca gtaagaatct
      541 taatgttctg ggtttcattt gtggcctcct cattccgttc tcaatacttc tgaatgtacg
      601 catcgttgct gctgtgtgtc aagcgaccag acaaggatga gagtaaccaa tcctatacgg
      661 gccgacatca ttcagcatgg atgcactccg gagcgtatga atcaaactgc tatcgcagat
      721 atccaaggtc cttcagattc tggagtacag ctggatccaa tttctcaggt tcgattttcg
      781 ttccagacac ggtggcttcc gaagagaggg acttggtgag caactgctgg agaatgcgtc
      841 cctcgattgg tttcgaaggc cacaagttga tcttctcgcc aggatcaacg ctgaggtcaa
      901 agagctcata ctcataccat ttgacctgag cactctcagg ggtactgagt ttccgaacaa
      961 gtttcatatc ccccttgcgg acgaaatgga tcagatttcg gactgcccca aggcgtgctc
     1021 gacccttgtc cgattcgtga gagcccagaa tcatcggcag aagactcttg ccctgcatct
     1081 gctcgggcgc tgatatgccg acaaagtcca ggactgtggg tgctacgtcc aaaagttgtg
     1141 cgggatagcc tgagtcagtt ccctctggga gaacgcctgg gagagaaaag accagtggaa
     1201 catgaactag ttcctggtac aaggaacgac cgtgttcaat gtcgccgtgc tccatgaaag
     1261 cttctccatg atcagaaaga aagaccacga gcgtgttgtg cgtcagattg ttctcctgca
     1321 gccagtcaga cagccaaccg acaaaccggt caacggaagt cacttcggcc ttgtacatgg
     1381 aagaaaagta ggcaagatcc tccggagaaa acctcatcaa accactctgc accatgtcca
     1441 tgatgtcttt gcccatgcct atcctacctt gatagttcgg agcatacatc tgagcgaatt
     1501 ccttgggggg tgtatacgga gtatggggat caatgagatg aatgtaggcg aaaaagggtt
     1561 gatccttcat gccttccaag gttggtttgc acaggtctgt ccagaaaccc ggatttccgt
     1621 cggaagagga tttgtgaagt gcatcgaaat ccggagcata ccattccacg actgtgtcaa
     1681 agccctgacc gtagccgaaa accggtgagg cttggctgtt ctgtatgatt gtggccgtcc
     1741 ggtatcccga ctctttcagc agctcaggca acgtaagaag actgtcactc aggatatcaa
     1801 gctgcgtcag aacgccatgc acccaaggga atgttgaggt cagaagcgag gcgactgacg
     1861 ccttcgtata actggcattg gtagcggcat cacgaaagag cacacccccc agagaaagcc
     1921 gatcgatgtt gggcgtct
//
LOCUS       DAOQUC010000842         1938 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_76195_length_1938_cov_1.852894, whole genome shotgun sequence.
ACCESSION   DAOQUC010000842 DAOQUC010000000
VERSION     DAOQUC010000842.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1938)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1938)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1938
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76195_length_1938_cov_1.852894"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..997)
                     /locus_tag="PKH07_13225"
     CDS             complement(<1..997)
                     /locus_tag="PKH07_13225"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1957 domain-containing protein"
                     /protein_id="HNT35946.1"
                     /translation="MKPIGSFTFVLHSHLPYVISHGRWPHGMDWLDEAAIATYIPLLN
                     VFSDLALEGISSKCTVGITPILSEMLSSDAFKNGLPVYLKERIRVSRQNVREFQRTGE
                     PQLKALARMWEQWYSNRLSDFEERYSRDLLGAFRGLQDSGHIEIITCAATHGYLPLLS
                     EDISIQAQIKAGVKTHRRHFGRNPKGIWLPECAYRPSYAWVSPLEVKGTPKVRKGVEE
                     FLSENGLEFFIVDSHLLHGGEAVGVYMDRFSALRRLWAQFEKEYTPAPVDFQKSPNDT
                     FLVGDKEGKKPVAILTRDDQTGLQVWSGEHGYPGDGAYLDFHKKYSQGGNRYWRIT"
     gene            complement(1094..>1938)
                     /locus_tag="PKH07_13230"
     CDS             complement(1094..>1938)
                     /locus_tag="PKH07_13230"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNT35947.1"
                     /translation="YNHSKRADVTISAVPRPIQDCSSLGVIQVDRQWRVTDFQEKPST
                     PSPIPGEPDMGLGSMGNYIFNTHILRQIIRFAHENDLHDFGRDIIPRILQDYKVYAYD
                     FRTNQIPGMTEAERGYWRDVGTIDSYYESNMDLRQVSPIFNLYNSEWPIRTAPTQAPP
                     GKFVFDDDGRRGMAIDSLVSEGCIISGSLVRNSVLSPNVFVHSYSSVDDSIVMSGVDI
                     GRNCRIRRAIIDKYVKVPSGEVIGYDLEQDSRKYHLSPGGIVVIAKHTQICSESASDA
                     GDSR"
BASE COUNT          484 a          519 c          476 g          459 t
ORIGIN      
        1 atgtgatccg ccaataccgg tttcctccct gactgtactt cttgtgaaaa tcgagatagg
       61 cgccgtcgcc aggatatcca tgctcaccac tccagacttg aaggcctgtc tggtcatcgc
      121 gtgtcagaat cgcaaccggc ttcttgcctt ccttatctcc taccagaaag gtatcgttcg
      181 gactcttctg aaaatccaca ggggcggggg tgtactcctt ctcgaactgt gcccaaagcc
      241 gtcgcaaggc actgaaccta tccatatata ccccaaccgc ctctccaccg tgaagcagat
      301 gggaatccac gatgaagaac tcaagtccgt tctcggagag aaactcttcc acacccttgc
      361 gaaccttcgg agtccctttc acttccagcg gagaaaccca ggcataggaa ggacgatagg
      421 cgcactcagg gagccatatc cctttcgggt tacgcccaaa gtgtcttcga tgtgtcttga
      481 ctcctgcctt gatctgagct tgaatactga tgtcctcaga gagaagggga agatatccgt
      541 gcgtcgcagc gcaggttatg atctcgatgt gacctgaatc ctgaagtcct cgaaacgcgc
      601 cgaggagatc cctgctgtag cgctcctcga agtcgctcag cctgttggag taccactgtt
      661 cccacattct tgcgagcgcc ttcaactgag gctcccctgt gcgttggaac tcacgaacat
      721 tctgacgtga gacacggatt cgttccttca ggtacacagg aagaccattc ttgaaggcgt
      781 cgctcgacag catttcagaa aggatcggcg tgatgccaac tgtgcacttt gaagatatcc
      841 cctcaagggc aagatctgag aagacattca gaagtgggat gtatgtcgca atagccgcct
      901 catccagcca gtccatcccg tgtggccatc tgccgtgtga aatgacatag ggcaaatgcg
      961 agtgcagaac gaaggtaaaa gaaccaatgg gtttcacaac aagatccttc ctgcccaaaa
     1021 ttggactgtg ggcttcagat taccaccaag tgccacgagc accaaatcca agagaactcc
     1081 ctcttgggat gattcatcgg gaatcgccgg catcggaagc actttcggaa catatctgcg
     1141 tatgttttgc gatcacgaca attccaccag gtgaaagatg gtacttcctg ctgtcctgtt
     1201 caagatcata tccgatgact tcgcccgaag gaactttgac atatttgtct atgatcgccc
     1261 ttcgaatacg acagtttctt ccgatatcca ccccagacat gacgattgaa tcgtcaacgc
     1321 tcgaataact gtgaacaaaa acattcggag agagaaccga attgcgaacg agagagccac
     1381 tgatgatgca tccttcagaa acaagcgagt caatggccat tccccgcctg ccatcatcgt
     1441 caaaaacaaa cttccctggc ggcgcttgag ttggagccgt tcgaatcggc cattcagaat
     1501 tgtacaagtt gaagatcgga ctgacttggc gcagatccat attggattcg tagtaggagt
     1561 ctatggttcc cacatcacgc cagtagcctc gctcggcttc cgtcattcct gggatttggt
     1621 tcgtccggaa atcataggca tagactttgt agtcttgaag aatccttggg ataatatcac
     1681 gaccgaaatc atgcagatca ttctcatgcg cgaatcggat tatctgacgg agaatgtgtg
     1741 tgttgaagat gtagttgccc atggatccca gtcccatgtc aggctcaccc ggaatcggag
     1801 atggcgtgga cggtttctcc tgaaagtcag ttacccgcca ttgacgatcc acctgaatca
     1861 caccgagtga ggagcagtcc tggatgggtc gaggaacggc ggagatggtc acatcagccc
     1921 gcttgctgtg gttatagt
//
LOCUS       DAOQUC010000843         1936 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_76299_length_1936_cov_2.705476, whole genome shotgun sequence.
ACCESSION   DAOQUC010000843 DAOQUC010000000
VERSION     DAOQUC010000843.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1936)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1936)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1936
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76299_length_1936_cov_2.705476"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(25..672)
                     /locus_tag="PKH07_13235"
     CDS             complement(25..672)
                     /locus_tag="PKH07_13235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35948.1"
                     /translation="MRVLSTLIAILLLGCSLTLADAADLTLEGSPYHPIVYVTAEDVA
                     RARSAIASETDAQAWYNTLLAEWSNWKDKSPEWIRDVMPDEGACFAYGFTGCPICGGR
                     WSAWGAAKCSFDNPGHVECSKGHILPNEKCPDAGTGYVASDGRIHYLVGSYNAWVVET
                     LCAKIASPCSDIYALSDDEQAGRMAAVILDEFARIYPSCDKGSWDYPSNPPSGRF"
     gene            complement(729..1088)
                     /locus_tag="PKH07_13240"
     CDS             complement(729..1088)
                     /locus_tag="PKH07_13240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018147387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HNT35949.1"
                     /translation="MQRKQEGFTFIEIMVVVGIIGFIVAIAAANYFTARKNAQGYMCT
                     ENLAKMHGAKQRWGFENNAEETDVPTFDDILPYLDTKEGQLAIECPAGGTYSLNPLNE
                     VPTCSLQSTEGRHSLQK"
     gene            complement(1283..>1936)
                     /locus_tag="PKH07_13245"
     CDS             complement(1283..>1936)
                     /locus_tag="PKH07_13245"
                     /inference="COORDINATES: protein motif:HMM:NF015426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35950.1"
                     /translation="QEFWKLGEGLNIALRDMEIRGTGNLLGRQQHGHISAVGFDLYHK
                     MLRETVTQMKGSETHEMYPETLLESPDRGEIPPTYVESPRQRLALYKRLAVLRTVQGL
                     DEFRDEIRDLYGPLPPETERLFTLRRLRSCARDVGIEYMNIGTHRVRMRFCDEAIKSF
                     DARVILNLANLNRFRIQMTTRDGLSLVVIWNEISEEEMLRDLQEIVEAFAKSGTGTS"
BASE COUNT          463 a          545 c          479 g          449 t
ORIGIN      
        1 tcttgccact tggtaccagg gtcgtcagaa ccgtccgctg ggtggattcg atggatagtc
       61 ccatgagcct ttatcacagg atggatagat acgagcaaac tcatccagaa taaccgccgc
      121 catccttcca gcttgttcat catccgagag ggcgtagatg tctgaacagg gtgaagcgat
      181 ctttgcgcat agcgtctcca ccacccatgc gttgtaggat ccaacgagat agtgaattct
      241 tccgtcagaa gccacgtaac ctgttcctgc atcaggacac ttctcgttgg gaagaatatg
      301 tcccttggaa cattccacat gccctggatt gtcaaaggag cacttagctg caccccacgc
      361 actccagcgt ccgccacaaa ttgggcagcc tgtgaaccca tacgcaaagc aggctccttc
      421 atccggcatg acgtctcgga tccattcagg ggacttgtct ttccagttgc tccattccgc
      481 caggagcgtg ttgtaccacg cctgagcatc ggtctctgat gcaatggcag atcgggcacg
      541 agcgacatct tccgccgtga cataaactat gggatggtaa ggtgatccct caagagtcaa
      601 atcggcagcg tctgccaaag tcagcgaaca acccagcaac aggatggcaa tgagagttga
      661 caagactcgc acgggacgaa tacctcgatt ctgtcagcag ttgagtgagc caacaagaga
      721 cagaaaggtc acttctgaag actgtgcctg ccttctgtgg attgcagtga gcaggtcggc
      781 acttcattca aaggattcag agagtatgtg ccaccggcag gacactcgat cgccaactgt
      841 ccctctttgg tatccagata gggaagaatg tcatcgaaag ttggtacatc tgtctcctct
      901 gcattattct caaaacccca tcgttgcttg gctccgtgca ttttggcaag gttctccgta
      961 cacatatatc cttgcgcatt cttgcgggca gtgaagtagt ttgccgcagc gatcgccaca
     1021 atgaacccaa tgattcctac gacgaccatg atctcaatga acgtgaatcc ctcttgtttg
     1081 cgttgcatga caggcctatc ctttctggca gacaccaaga attggcgacc cgacaatggc
     1141 aacgttttct ggagaaacca gatcccggcg gaatgcggct tctgtatcat aagcacttgg
     1201 gtggacggtg tcttccacac gcccatctgt gccacccgct ctgcagcaca ggagtttgta
     1261 taacactaat gggcaagaaa agtcaagaag tgcctgtccc gcttttcgcg aaggcctcga
     1321 cgatctcctg aaggtcacgg agcatttctt cttcggagat ctcattccag atgaccacca
     1381 aactgagacc atcccgggtt gtcatctgaa tccgaaaccg attgaggttg gccaagttca
     1441 gaatgacacg agcatcgaaa ctcttgatcg cctcatcgca gaagcgcatt cgcacccgat
     1501 gtgtgccgat gttcatgtac tcgataccca catcccgtgc acagctgcgc agccgacgaa
     1561 gagtgaaaag acgttccgtt tccggcggca gcggcccata cagatcccga atctcgtcac
     1621 gaaactcatc cagcccctga acggtgcgca gaacggcaag cctcttgtaa agtgctaaac
     1681 gctgtcgcgg actttccaca taggtggggg gaatttcccc gcgatccgga gattccagga
     1741 gtgtttcggg atacatctcg tgtgtctcgg atcccttcat ctgcgtcacc gtctctcgga
     1801 gcatcttatg ataaagatcg aatccaacag cggagatgtg cccgtgctgt tgtcgcccca
     1861 gcagattgcc ggtgcctcga atttccatgt cgcgaagcgc aatgttcagg ccttcaccca
     1921 gtttccaaaa ctcctg
//
LOCUS       DAOQUC010000844         1935 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_76350_length_1935_cov_3.240426, whole genome shotgun sequence.
ACCESSION   DAOQUC010000844 DAOQUC010000000
VERSION     DAOQUC010000844.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1935)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1935)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1935
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76350_length_1935_cov_3.240426"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..987)
                     /locus_tag="PKH07_13250"
     CDS             complement(<1..987)
                     /locus_tag="PKH07_13250"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNT35951.1"
                     /translation="MRSKPDKNNKTTEPKSRDDGTTDSRNRPPTADRMQTGFQELSHF
                     FECNLDLLCIVDTDGHFLRMNREWEKVLGYSVSELENRPFMDFVHPDDREATLQTFGR
                     LTAQEEVLSFENRYRHKDGSYRWLEWRSVPQGNIIYAAARDITERKRNEKALHDREDY
                     LQTILQTTADGFWVVDTKGRVIEANKAYCVMTGYSREEILSLRINDLDAVESSEETAA
                     RIRRVMQAGTETFETSHRRKDGSLFPVEVSTSFVSEYGGQFVCFGRDLTERRQREERI
                     RLLGKMLDDAPASITIHDTDGHFLFANQQTISLHGFQSEADFLGVNLHDLDVP"
     gene            complement(1423..>1935)
                     /locus_tag="PKH07_13255"
     CDS             complement(1423..>1935)
                     /locus_tag="PKH07_13255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004074674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNT35952.1"
                     /translation="IVQQNHGFINVYSELGHGTTFRIYFPQHQQQAQESPREESLRQI
                     SHGNETVLLVEDESAILRLAQTMLERWGYRVLTASKPSEAIQIAQSYPESIHLLITDV
                     IMPEMNGRDLTRHIASLRPGMKVLFTSGYTANVIAHHGVLDAGVHFMQKPFSMEDLAH
                     KVREALDTVL"
BASE COUNT          425 a          525 c          486 g          499 t
ORIGIN      
        1 gggcacatca aggtcgtgca aattgacacc gagaaaatcc gcttcgctct ggaatccatg
       61 cagactgatc gtctgctggt tggcaaagag aaagtgcccg tcggtatcat ggatggtgat
      121 agaagcaggg gcatcatcga gcatctttcc cagcagcctg atacgctctt cgcgttgtct
      181 ccgctctgtc agatcgcgac caaaacagac aaactgccca ccgtattcgg aaacgaaact
      241 ggtggatacc tcaaccggaa atagactgcc atcctttcga cggtgggaag tctcgaatgt
      301 ttccgttccc gcttgcatga cgcgccggat tctggccgcg gtctcttccg aactctctac
      361 ggcatcgaga tcatttatcc tcagactaag aatctcctca cgactgtacc ccgtcatgac
      421 acagtaggcc ttgttggctt caatgactct tccttttgtg tcaacgaccc agaatccgtc
      481 cgcagtcgtc tgcagaatcg tttgcagata gtcttccctg tcatgcagag ccttctcgtt
      541 tctttttcgc tcagtgatat ctcgtgctgc ggcgtagatg atgtttcctt gaggaacgga
      601 gcgccattcg agccagcgat aactcccatc tttatgccgg tagcgattct caaagctcag
      661 aacctcttct tgcgcggtga gtcggccaaa agtttgcagt gtggcttccc gatcatctgg
      721 atgaacaaag tccataaacg gcctattctc cagttcggaa acagagtagc ccagaacctt
      781 ctcccattcg cgattcattc gaaggaaatg gccgtctgtg tcaacaatgc acaacaagtc
      841 gagattgcat tcaaagaagt gactcagctc ctgaaatccg gtctgcattc tgtctgcagt
      901 tgggggacga ttgcgggagt ccgtcgttcc gtcgtctctg ctcttgggtt cagtagtctt
      961 gttgttcttg tctggttttg atctcattgt gagctccttc acaacgcagg tcataggagc
     1021 gttcttggat tccacctctg tcagaacttg gcgcactcaa agaaattcag cacgatccta
     1081 atcaaaggta tcaggttcac agatcagcca atgctcgtat gacgacaaac ggtggctcct
     1141 tagcgcgagc attcctcttc acgcgtcaac tcagtctact gtatccaatt cactcatcgt
     1201 tttccagtga aaacggccgc tgttggtatg ggctcagtta cgctggcgct acagccagga
     1261 tatgcttcgg aagcaaagcg gagtcttcgg agcgacgcta ccgaagcccg ctttctgtct
     1321 ggtcccgctt cggcagcgtc gcagagcctc tgcaaccgaa gcatacctcc cccccctgcg
     1381 taactgagcc agtaccactc ttagaccggg gtttcaggag acctagagaa ccgtatcaag
     1441 cgcttcgcgg actttgtgtg ccagatcttc catcgaaaac ggcttctgca tgaagtgaac
     1501 gcctgcgtcc aggacaccgt ggtgggcaat gacattggcc gtgtaaccgg acgtgaacaa
     1561 gactttcatc ccgggacgca gagacgcgat atgtcgggtc agatcccgcc cattcatctc
     1621 cggcatgatg acatccgtga tcagaagatg gatgctttct ggataggatt gtgcgatctg
     1681 tatggcttca gatggtttgg aagcagtcaa caccctgtat ccccatcgtt caagcattgt
     1741 ctgtgccagc ctgagaattg ctgattcatc ttccacgagc aggacagttt cgttgccgtg
     1801 actgatttgt cgtaaggact cttctcgggg tgactcctgc gcctgttgct ggtgttgcgg
     1861 gaagtagatc cggaacgtcg tgccatgtcc cagttcactg tagacattga tgaagccatg
     1921 gttttgctga acgat
//
LOCUS       DAOQUC010000845         1935 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_76365_length_1935_cov_2.482979, whole genome shotgun sequence.
ACCESSION   DAOQUC010000845 DAOQUC010000000
VERSION     DAOQUC010000845.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1935)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1935)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1935
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76365_length_1935_cov_2.482979"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            137..487
                     /locus_tag="PKH07_13260"
     CDS             137..487
                     /locus_tag="PKH07_13260"
                     /inference="COORDINATES: protein motif:HMM:NF018654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged-helix domain-containing protein"
                     /protein_id="HNT35953.1"
                     /translation="MITNKNCIIRLSRYKNALYRLRSLGFVKVFSENLADAVGVTASQ
                     VRKDFSLFGISGNRRGGYEVSLLLEKLGCMNRQIAQIYGTPQKKSLDGSVVHKLLHDV
                     RCRETGDDNLAGHV"
     gene            complement(401..1090)
                     /locus_tag="PKH07_13265"
                     /pseudo
     CDS             complement(401..1090)
                     /locus_tag="PKH07_13265"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
     gene            1385..>1935
                     /locus_tag="PKH07_13270"
     CDS             1385..>1935
                     /locus_tag="PKH07_13270"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HNT35954.1"
                     /translation="MTIRVQNVSEPDVHVQDSQYDIGEEQIERIFECFPLEVKDRKVL
                     VKPNLLGLFTPDKAVTTHPSLVRSVVQCLKRRGACVTVGDNPGLRGYGDNERIARFTG
                     IHDAVVDCYQNIAKRSTQVTLKNRRNDSVLVSTAVLESDLIVSLPKLKTHMLTQITCG
                     IKNTFGYLVGGEKTKLHSLSHAGR"
BASE COUNT          476 a          478 c          469 g          512 t
ORIGIN      
        1 tttactatag ccgctgtttc tgcagcatat ctgtttgtct ggctcatctg gtataaataa
       61 aaattacaca taaaacttga caaatttaaa aaatatgtta atgtttgcta tgttattgtg
      121 aaataaatca caatatatga ttactaataa gaattgtata atcaggcttt caaggtacaa
      181 gaacgccctc tatagactgc ggtctttagg gtttgttaaa gtattttcgg agaacctggc
      241 agatgcagtc ggcgttaccg cttcccaggt gcgcaaggat ttctcgctct ttggtatttc
      301 cggcaacagg cgcggaggct acgaggtaag ccttctcctt gagaagcttg gctgcatgaa
      361 ccgtcagata gcccagatct acgggactcc acagaagaaa agtcttgacg gttccgtcgt
      421 ccacaaattg cttcatgacg ttcggtgtcg agagaccggt gatgacaatc ttgccggtca
      481 tgtctgactg tcgtgctgcc aatgctgctc ccggcagagc gaccgaagta atcccgaaga
      541 cacctttgag attcggatat gctttgaaga gatcttgagt cacctgaacg gcaagttgct
      601 gatcctcttc agaaggcttt accgttacga tcttcatctc agggtatttg tcttcgatgc
      661 gctttctcat gtgttccatc cagatattct ggttggcggc agtaagggag ccagtaacaa
      721 ttgcggtctc tcccttacca cctatttgct ctgccatgac atccaccaga ccatgaccga
      781 tggattcgta ggttgcctga ttgacgaaat agtccctgct cttgtggtcg gagtctgcgt
      841 cccatgtcaa aacgcggata cctctcttga gcgccttctt caatgttggg gcaagagcgt
      901 tgggatcatt gggtgcgacc gcaatgacat ccatctttct catgatccag gtgtcgagca
      961 tctctgcctg cttggtgacg tcattggaga ttggaccttc gtagacaatg ttcacgccca
     1021 attcggctgc ggcttcacgg gcaccctttt cggcagcatt aaaatagtct atgccaacga
     1081 gttttggcat caccccaatc gtgatggacg gcttctcttg agccacagtc tgacccagaa
     1141 acacaaaaag tccgaccaat acgatcccaa ccgacagcga ttttctcatc ttgctcctcc
     1201 atcatgaact gctttaagtc caagcagttg ctgtacagac atatcaagaa tcctatcaca
     1261 aaactctatc agattctcca cttaaggcgt agccaaaatc ctccgaattc atctgacggt
     1321 tgacaccgtg ggcacggagg ctctagcatc tgccctcaac aaatcactgg tcaaggagtt
     1381 ttgcgtgaca atacgagtgc agaatgtctc cgaacctgat gtgcatgttc aggattcgca
     1441 atatgatatt ggcgaggagc agatagagcg cattttcgaa tgctttcctc ttgaggtgaa
     1501 ggatcggaag gtccttgtga agccgaatct gcttggcctc tttacacccg acaaggcggt
     1561 gacgacgcac ccatcattgg ttcgatccgt ggtgcagtgt ctcaagaggc ggggggcttg
     1621 tgtgacggtt ggcgacaatc cgggtcttcg cgggtacggg gacaacgagc ggattgctcg
     1681 atttaccggt atccatgatg ccgttgtaga ctgctaccag aacatagcca agcgatccac
     1741 gcaagtcact ctcaagaaca ggagaaacga ctcagttctg gtttcgacag cggtgctgga
     1801 gagcgatctg atcgtctcgc ttccaaaact caagacccac atgttgacac agatcacttg
     1861 tggtatcaaa aatacctttg gttacctggt tggaggagag aagacgaagc tccactccct
     1921 gagccatgca ggtcg
//
LOCUS       DAOQUC010000846         1935 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_76372_length_1935_cov_2.331915, whole genome shotgun sequence.
ACCESSION   DAOQUC010000846 DAOQUC010000000
VERSION     DAOQUC010000846.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1935)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1935)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1935
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76372_length_1935_cov_2.331915"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1079)
                     /locus_tag="PKH07_13275"
     CDS             complement(<1..1079)
                     /locus_tag="PKH07_13275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35955.1"
                     /translation="MLRIGNARQLFLDDFVVAELRGVVRTLHQPTKYENNPVVVPEHP
                     WEDQGINYPLVFRDGSIYRMYYLARGSAKDTPDGSGNFGPVGYAESQDGIHWTKPLLD
                     VYSLPGHPKTSLGFALTQPPLRQVEGPAIIKDERDPDPQRRYKLFFSKGRDRVTPTEG
                     FSVAFSPDGLRWKEYDGNPVYKLYGDTQNCLVWDPEIERWVAFVRMWGKPSFQRPVWF
                     FKKYRQRRIRFVARMSSADFEHWTPSEVVLTPDPSDSRVCDFYGIQVTYYEGLYIGFL
                     WVLTSIGGEEPGSHGYLNAQLVTSRDHGLTWHRVCGRKPVLDRGKQGSFDFGGVYPVP
                     FVTEDKEHLVYYKAIDQQHGVSCNST"
     gene            complement(1092..>1935)
                     /locus_tag="PKH07_13280"
     CDS             complement(1092..>1935)
                     /locus_tag="PKH07_13280"
                     /inference="COORDINATES: protein motif:HMM:NF028222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HNT35956.1"
                     /translation="SVGDGVSEFAVGERVTVEPLLVCNECDRCKSGRYNACESLRVMG
                     CHADGAYADFLAVPAEKVLRLPDSVSFEDGALIEPAAVAHHAARRANLKGGESILLFG
                     AGPIGLFTLQMLKAYGAGAITCVDLSRYRLQLALSLGADGVVDARESELACLKHRRAE
                     GKEFDLIVDCVGGKGKALDAALDIAAKGSRILAVGILEQGVCMQYLTWIAEHELELIG
                     SFTYVREDFERTIQLLEKGSVRTQGIGSHRFKMEDAAKAFRFIESPDERFVKAILVND
                     AYTG"
BASE COUNT          491 a          562 c          460 g          422 t
ORIGIN      
        1 gtggaattac acgaaacacc atgctgttga tcaatggctt tgtaatagac cagatgctcc
       61 ttatcctcgg taacgaaggg cacgggatag actccgccga agtcaaaact tccctgcttt
      121 ccccgatcca gaaccggttt gcgtccacac acacgatgcc aggtcagacc atggtcgcga
      181 ctggtaacca attgggcgtt cagatacccg tggcttccag gttcttcgcc gccaatgctg
      241 gtcaagaccc agaggaatcc aatgtacaaa ccttcatagt acgtgacctg aatgccgtag
      301 aaatcacaca cgcggctgtc ggatggatcg ggcgtcaaga cgacctcaga aggcgtccaa
      361 tgctcaaagt ctgcactcga catccttgca acaaatcgga ttctgcgctg tcggtacttt
      421 ttgaagaacc aaacaggtct ctgaaaactg ggtttccccc acattctcac aaaagcaacc
      481 caacgctcaa tctcaggatc ccagacgagg caattctgcg tgtcgccgta gagtttgtag
      541 acgggattcc catcatactc cttccatcga aggccgtccg gtgagaatgc gactgaaaaa
      601 ccttccgtgg gagtgactcg atcgcgacct ttgctaaaga aaagtttgta gcgcctttga
      661 ggatcgggat cgcgttcatc tttaatgatc gccggacctt ccacttgcct caaagggggc
      721 tgcgtcagag caaagcccaa acttgttttc ggatgacctg ggagtgaata gacatcaagc
      781 aggggtttgg tccagtgaat cccgtcttgg ctctcggcat agccgactgg tccgaaattc
      841 ccggacccat cgggagtgtc tttggcactt ccgcgagcaa gatagtacat gcggtagatc
      901 gaaccatccc gaaagaccaa gggatagtta atcccctggt cttcccaggg atgctccgga
      961 acaacgacgg gattgttctc gtatttcgtg ggctgatgaa gagtccgcac aacaccacgc
     1021 agttcagcaa caacaaagtc atcaaggaac aactgtctcg cattgccaat tctcagcata
     1081 ttttgaaaac ctcatcctgt ataagcatcg ttcaccagaa ttgctttgac aaaacgctcg
     1141 tcaggggatt cgatgaaacg aaacgctttt gcggcgtctt ccatcttgaa ccggtggctg
     1201 ccgatcccct gggtgcgaac gcttcccttc tccagcagtt gtatggttcg ctcgaaatcc
     1261 tctcggacat aggtgaacga accgattagt tccagttcgt gttcagctat ccaggtcaga
     1321 tactgcatac acacaccttg ttccaagatg ccaacggcaa gaatgcgtga cccttttgca
     1381 gcaatgtcca aggccgcatc cagagccttc cctttgccgc cgacacagtc aacgatcaag
     1441 tcaaactctt ttccttcagc tcggcggtgc ttcagacacg cgagttcact ttcacgtgca
     1501 tcaacaacgc cgtctgcacc aaggctcagt gccagttgca ggcggtaacg agacaaatca
     1561 acacaggtga tcgctcccgc cccgtacgcc ttcagcatct gcagcgtgaa cagtcctatc
     1621 ggtccagcac cgaacaagag tattgattcg ccgcctttga gattggcgcg acgagcagca
     1681 tgatgcgcca cggctgcggg ctcaatcaaa gcaccatctt caaaggaaac cgaatcagga
     1741 agccgcaaga ccttctccgc cggaacagcc agaaagtcag cataagcgcc atccgcatgg
     1801 catcccatca ctcgaaggct ttcgcacgca ttgtaccggc cactcttgca gcgatcgcac
     1861 tcattgcaga ccagaagtgg ctccacagta acacgttcac cgacagcaaa ctcagacact
     1921 ccatcaccga cggag
//
LOCUS       DAOQUC010000847         1929 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_76721_length_1929_cov_3.670224, whole genome shotgun sequence.
ACCESSION   DAOQUC010000847 DAOQUC010000000
VERSION     DAOQUC010000847.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1929
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76721_length_1929_cov_3.670224"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..535
                     /locus_tag="PKH07_13285"
     CDS             <1..535
                     /locus_tag="PKH07_13285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35957.1"
                     /translation="KGPRSGVLALWDEMTSVRPIVGIAGLDAHAQRLVGLTAFPYEFL
                     FRTLRTHVLCCPPTGNSADDVAAVLDSIRLGHVYFSNDLYWSPKGFAFSASVGGKDYL
                     QGDVITDSGKIRLSVSLPDDAAIRVLRDGELWTCMEGRSWEDETSQHGVYRVEVSRES
                     QPWIFSNPIYFRNIPFI"
     gene            877..1842
                     /locus_tag="PKH07_13290"
     CDS             877..1842
                     /locus_tag="PKH07_13290"
                     /inference="COORDINATES: protein motif:HMM:NF013607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HNT35958.1"
                     /translation="MSVKSFKILIVCTGNTCRSPLGEGLLRQSLVSEGFQGVSVISAG
                     TSAVPGMPPSKEAVEVAREFGVDISEHTSLPLSAALIERANLVLAMGEGHKECIYSLS
                     PSSRGKVFLAGGFRRNAPPAEILDPIGGSVDLYRTCAQTLQESFQGLLHQLPILQSEF
                     ASDVVDFAVGTDHRGIPVKKILLSVLDRMNMTWEDMGAFSQEPSDYPNFAFAVGERVV
                     QEKARRGALLCGTGIGMSIAANKIRGVRAAVVTNARLAQLSRTHNNTNVIVFCEDTTE
                     AQLEELIGVWWNAEFEGGRHAKRVGLIVDYENLHSRDSQAGDWNA"
     gene            1835..>1929
                     /locus_tag="PKH07_13295"
     CDS             1835..>1929
                     /locus_tag="PKH07_13295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013537527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HNT35959.1"
                     /translation="MPRPGWDEYFMQIAELVKTRSTCLRRQVGCVA"
BASE COUNT          438 a          432 c          534 g          525 t
ORIGIN      
        1 taaggggcct cgttcgggag ttcttgccct ctgggatgag atgacttctg ttcgaccgat
       61 cgtgggcatt gcgggtttgg atgctcatgc gcaaaggctc gtcggtctaa ccgcgtttcc
      121 atacgaattc cttttcagga cattgagaac ccatgtcctt tgttgccctc ctaccggcaa
      181 ttctgcggac gacgttgccg cggtgcttga ctcgatacgg ctaggacacg tatacttctc
      241 aaacgatttg tattggtccc cgaagggatt tgccttttct gcttcagttg gtggcaaaga
      301 ttatcttcag ggggacgtca tcacggatag tggaaagata cgtctgtctg tttcccttcc
      361 agacgacgcg gcgattcgag tgttacgaga tggagagcta tggacttgca tggaaggaag
      421 gtcttgggaa gatgagacgt ctcagcatgg ggtgtatcgg gttgaggtgt ctagagaatc
      481 ccagccatgg attttctcca atccgatcta ctttcgaaat atccccttta tctgagggct
      541 cttgcacgag ttttccacaa tcctggcttc gatttccagg gatttctgtg gatgattctg
      601 tggaaaaact gctcagaagc ccatccagac ttgtgttggg ctttcagagt gactttttca
      661 gattagaatg agtattaaga aatgatatat aagatattgt atatgaatat attatgtttt
      721 agatattcgt cagcatcaat ggtgacgctc cttatgggct atccatgcct gatgagcaga
      781 ttcttgagtt ctgtcaaggt gagcgtaacc cttttggagc cttggttttg aggcgctatg
      841 cgaggagttt tccacaattt atgcacaggg tgaatcatga gtgttaagag tttcaaaatc
      901 cttatcgtat gcacaggcaa cacctgcaga agtccgctgg gtgaaggact cctgaggcaa
      961 tcgctcgtgt ccgaaggctt tcaaggcgtt tcagtgattt cagcggggac ttcagccgtc
     1021 cctggcatgc ctccttcaaa ggaggctgtc gaagtagcaa gagagtttgg ggttgacatc
     1081 tcggaacaca cttctcttcc tctttctgcc gcactgatag agagagcaaa tcttgtgctt
     1141 gccatgggcg aaggacacaa ggagtgcatc tattcacttt cacctagttc aaggggcaag
     1201 gtgtttctag ctgggggatt tcgaaggaac gctccccctg ctgaaatact ggatcccatc
     1261 ggtggatctg ttgacctgta ccgaacctgt gctcaaacct tgcaggagtc tttccagggg
     1321 ttacttcacc aacttcccat actgcaatct gaatttgctt cggatgttgt tgattttgct
     1381 gtgggtactg accatagggg gattccagtc aaaaagattc tactatcagt ccttgaccgc
     1441 atgaacatga cttgggagga catgggagcc ttctctcagg agccgtccga ctaccccaat
     1501 tttgcctttg cagtcgggga gcgtgttgtt caggaaaagg cgcgtcgcgg ggcactccta
     1561 tgtggtacgg gtataggcat gtccatcgcc gcgaacaaga tcaggggagt tcgagcagca
     1621 gtggtcacaa atgctcgact tgcccagttg agccgaacgc acaacaatac caatgtcatc
     1681 gttttctgtg aggataccac agaggcgcag ttggaggagt tgataggcgt ctggtggaac
     1741 gcggagtttg aagggggcag acacgccaag cgtgtcgggc tgattgtaga ctacgagaat
     1801 ctgcactcaa gagattctca agcgggagac tggaatgcct aggcctggtt gggacgagta
     1861 tttcatgcaa attgccgagc tggtcaagac gcgctctacg tgtctgcgaa gacaagtagg
     1921 atgtgtcgc
//
LOCUS       DAOQUC010000848         1927 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_76862_length_1927_cov_3.184829, whole genome shotgun sequence.
ACCESSION   DAOQUC010000848 DAOQUC010000000
VERSION     DAOQUC010000848.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1927
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76862_length_1927_cov_3.184829"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1927)
                     /locus_tag="PKH07_13300"
     CDS             complement(<1..>1927)
                     /locus_tag="PKH07_13300"
                     /inference="COORDINATES: protein motif:HMM:NF026288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35960.1"
                     /translation="LLKTLAQEYPNEPEVLEQLGTLLTKCGEHREAFEILTKAAALYR
                     QSSRFDSAATCYRDAMQSSNTQTADLTVKTWREFAEMYLTMGRFGEALGIFREAAESL
                     LSLGLQDKIQEFLRGLADQNPQAGQAFESLADLFLEKGYAEHARWCLLQAAEFYASHQ
                     WFSKAQQIYETLVEKDPSLTEAATRLTQIYRQRGLDSEAATFCLHILQDSLVGKADAE
                     LRQFYLDLARQCQPESIAVLEASVGFHESALSLARGKKEQQAIGKELASLQCRLGERF
                     LSENDFESAVCHLQAAMELDAKNPQVLALSEKIEKRRIERAPTEAAAEGIQKAEELVS
                     KKKFADAAKLLETLLQSDPYNLRVTLDLCDVLVGQKKEDEAFSLMQEQGAHFVAENLL
                     TSAQKCLERCCNLQPDEPESHLSLAGVLSRLGHREGAVKEYEKVLNLAEEKDLDELAL
                     NASRAALALEPDNLRFGQKQAMLLESSGETEQARLSWLTLSQRARERADEESRARFLQ
                     RALSVWPEDLQVEERIPVQQELAQSFVSLGRLEQAATLFDQSAHAARSVEQWNLLAKG
                     LEFVLADDRISSSLRGRQGLEWLVEAYEKTKNADKAVGVWRQLARHLFEQGDCAGALK
                     AYRSLLALQEGDLDALEQ"
BASE COUNT          439 a          562 c          484 g          442 t
ORIGIN      
        1 ctgttccaag gcatccagat cgccctcctg cagcgcaagc aggcttcggt aggccttgag
       61 ggcaccggca cagtcaccct gttcaaagag gtgtcgagcc agttgacgcc aaactccaac
      121 agccttgtcg gcattcttgg ttttctcata ggcctcaaca agccattcga gtccttgcct
      181 gccacgcaac gatgaagaga ttctgtcatc ggcaagaacg aactcaagtc ctttggccag
      241 cagattccat tgttccaccg agcgagcggc atgagcactt tgatcgaaga gcgtggccgc
      301 ctgctccaat cggccaagac tgacaaatga ctgagcaagt tcctgttgaa ccgggatgcg
      361 ttcttcaacc tgaagatctt caggccagac cgacagtgct ctctgaagga acctggcacg
      421 gctttcctca tctgctcgct cccgcgcccg ttgactgagt gtgagccagg agagacgcgc
      481 ctgctccgtc tcaccagatg attcaagcaa catcgcctgc ttctgcccaa aacgaaggtt
      541 gtcaggttcg agagcaaggg ctgcgcgact ggcattgagc gccagttcgt caagatcttt
      601 ctcttccgcc agattcagaa ccttctcata ctccttgact gcgccttctc gatgacccag
      661 acgcgaaagc acgccggcga gcgacaggtg tgattcaggc tcatcgggtt gaaggttgca
      721 gcatcgctcc aggcacttct gtgcactggt caggagattc tcggcgacaa aatgcgctcc
      781 ctgctcctgc atcagggaaa aggcttcatc ttcttttttc tgccccacaa gaacatcgca
      841 caaatccaac gtcacgcgaa gattatacgg gtcggactgg agaagcgttt cgagcagttt
      901 agccgcgtcc gcgaacttct tctttgaaac aagctcctcg gccttctgaa taccttctgc
      961 ggcggcttcg gtcggagctc tttcgatgcg tctcttctct atcttctcag aaagcgccag
     1021 aacctgaggg ttcttggcat ccagctccat cgcagcctga agatgacaca cggcagactc
     1081 aaagtcattc tcagaaagga agcgctctcc aagtcggcat tgcagtgaag cgagttcctt
     1141 cccaatcgcc tgttgttcct tcttgcctcg ggcaagggac agcgctgatt catgaaaacc
     1201 cacagacgct tccagcacgg cgatggattc gggctgacac tgtcgcgcga gatcgagata
     1261 gaactgccgg agctcagcgt ccgccttccc gaccagagaa tcctggagaa tatgaagaca
     1321 gaatgtagca gcctcagagt ccaatccacg ttgtcggtaa atctgcgtca gacgtgttgc
     1381 cgcttcagtg agcgaaggat ccttttcgac gagagtctca tagatctgct gggcttttga
     1441 aaaccactga tgagaggcat agaactcagc ggcctgaagc aggcaccacc gagcatgttc
     1501 tgcatatccc ttctcaagga acagatcagc aagagattcg aacgcttgtc cagcttgcgg
     1561 attctgatca gccaaccccc tcaggaattc ctgaatctta tcctgcaagc cgagactcag
     1621 caatgactcg gcagcctcac ggaaaatccc caatgcttcg ccaaaacgcc ccattgtgag
     1681 atacatctca gcaaactcac gccaggtctt caccgtgagg tctgcagttt gtgtgttcga
     1741 cgactgcatc gcatctcgat agcatgtggc cgcagagtca aatcggctgc tttgtcggta
     1801 caacgctgct gccttcgtca ggatttcgaa ggcttcccga tgttctccac attttgtgag
     1861 gagcgttccg agttgctcca agacttccgg ttcattggga tactcctgtg caagagtctt
     1921 gagcaac
//
LOCUS       DAOQUC010000849         1927 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_76869_length_1927_cov_3.034188, whole genome shotgun sequence.
ACCESSION   DAOQUC010000849 DAOQUC010000000
VERSION     DAOQUC010000849.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1927
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76869_length_1927_cov_3.034188"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="PKH07_13305"
     CDS             <1..412
                     /locus_tag="PKH07_13305"
                     /inference="COORDINATES: protein motif:HMM:NF014494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HNT35961.1"
                     /translation="ETFCALTGHPVYTEMFTAHQQRSLDHIHLAEWADALIIAPATAN
                     ILGKMSCGVADDLLSTTFLACSCPVGLVPAMNDQMYAHSIVQRNVQVLKDIGCRVLEP
                     VEGQLASGKVGRGRMPEPEAVFEWIVEIMTQQGK"
     gene            440..>1927
                     /locus_tag="PKH07_13310"
     CDS             440..>1927
                     /locus_tag="PKH07_13310"
                     /inference="COORDINATES: protein motif:HMM:TIGR03662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2298 domain-containing protein"
                     /protein_id="HNT35962.1"
                     /translation="MLFLWYIIISLIGLIAFPLAYVTCVGFKDRGWGISRALGLLLVV
                     YPTWIWAQMHFIPFSRFSTVLSVLAILALSIFVAWNRKQDLLSFFRNNWRTVAATELV
                     FLCAFIFMALVRMRNPEIASRWQGYGSEKLMDMNFVNSILRSRWFPPMDNWFSGHSIN
                     YYYHGYYLTAFLTLLTNHPSEYTYNLMVVLLFALAFVGCYCVGRNLTGKPLCGILCAL
                     VVLVMGNLQGAVLVWKDMPDAKWPARMASVWQYGKRGFIPSDVWACSRVIDLPNDRTI
                     NEFPYFSFLWGDLHGHLVSLPFCVLLVALLANAQWLGAGNSAHSPPGQRSSWWIALMI
                     AIVLGGVAAISSWDLPTYSLLTLGAGFAFVGRARVGAWLREWGLRLFAVAGGIIFYLP
                     FYLHFVAPSKGMNRVTQKSTLDKILIIFGLHLFLVVTYLILRLVRNASESDRERIRTL
                     GLVLGITLLVMLIVIAVGYQITSQWVVGPLGAVLVFVLMLYGLTFL"
BASE COUNT          363 a          486 c          539 g          539 t
ORIGIN      
        1 cgagaccttt tgcgccctca ccggccatcc agtctacacc gagatgttca ccgcacatca
       61 gcaacgttcc cttgaccaca tccatctcgc ggaatgggcg gatgcgctga tcattgctcc
      121 ggcaacggct aacattctcg gcaagatgtc ctgtggcgtc gcggatgatc tgctttcgac
      181 aacatttctg gcatgctcgt gtccggtggg tcttgtgccg gcgatgaatg accaaatgta
      241 cgcacattca atcgtccagc gaaatgttca ggtactgaag gacattggat gtcgtgttct
      301 ggaaccggtt gaaggacaac ttgcgagtgg caaggtcggt cgggggcgaa tgccggaacc
      361 agaagcagtt tttgaatgga ttgttgagat catgacccaa caagggaaat gaacaatact
      421 tcattttgaa gagggtgcga tgctgtttct gtggtacatc atcatctcgc ttatcggttt
      481 gattgctttc ccgctggctt acgtgacttg cgtcggattc aaagatcgtg gatggggaat
      541 ttccagggcg ctggggcttc tgttggttgt ctatccaacg tggatctggg cgcagatgca
      601 cttcattccg ttcagccgat tctccacggt tctctccgtt ttggcgattc tcgcactgtc
      661 catatttgtg gcatggaaca ggaagcagga tttgctttct tttttccgga acaactggcg
      721 gactgtggct gcaaccgagc tggtctttct ctgtgccttc atctttatgg cactggttcg
      781 catgagaaat cccgagattg ccagccggtg gcaggggtac ggttccgaga agctgatgga
      841 catgaacttt gtcaacagca ttcttcgaag ccgttggttc ccaccgatgg acaattggtt
      901 ctcgggacac tcaattaact actattacca cggctactac ctgacagcgt tcctgacact
      961 actgaccaac catccatccg aatacacgta caatctgatg gtcgtgcttc tgttcgccct
     1021 tgcatttgtc ggttgttatt gcgtgggcag gaatctgacg ggcaagcctc tgtgcggaat
     1081 cctctgcgcg ctagtggtgc ttgtaatggg caatctgcaa ggcgctgtcc tggtctggaa
     1141 agacatgcct gacgcaaaat ggcctgcccg aatggcttcg gtctggcagt acggaaagcg
     1201 ggggttcatc ccctcagacg tctgggcttg ttcacgagtc attgatctgc ccaatgatcg
     1261 aaccatcaac gagttcccct atttcagttt cttgtggggc gatctgcacg gtcaccttgt
     1321 aagccttcct ttctgtgtgc ttctcgtggc tctgttggca aatgcgcagt ggcttggtgc
     1381 gggcaattca gctcacagcc ctcctggaca gcgaagcagc tggtggattg cgctcatgat
     1441 cgcgattgtt ctgggcggcg tcgctgcgat cagcagttgg gatttgccca cttattccct
     1501 tctgacgttg ggcgctgggt tcgcttttgt cggtcgcgcc cgagttggag cctggttgag
     1561 ggaatggggg cttcggttgt tcgcggtggc gggtggcatc atcttctacc tgccttttta
     1621 cttgcatttc gtcgcgcctt caaaagggat gaaccgagtt acccagaaat ccacgctgga
     1681 caagattctg atcatttttg ggcttcattt gttcctcgtc gtgacctatc tgattctgcg
     1741 tcttgtccgg aatgcctcgg aaagcgatcg cgagaggatt cgaaccttgg gcttggtgct
     1801 ggggatcact ctgcttgtga tgcttatcgt catcgcagtc ggctaccaga taacatccca
     1861 gtgggtggtt ggacctttgg gagcagtatt ggtcttcgta ctgatgctct atgggctgac
     1921 attcctg
//
LOCUS       DAOQUC010000850         1925 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_76994_length_1925_cov_3.726203, whole genome shotgun sequence.
ACCESSION   DAOQUC010000850 DAOQUC010000000
VERSION     DAOQUC010000850.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1925
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76994_length_1925_cov_3.726203"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            113..1681
                     /locus_tag="PKH07_13315"
     CDS             113..1681
                     /locus_tag="PKH07_13315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020035687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HNT35963.1"
                     /translation="MQLNEAVKSVFASGNFTSLDWGIVVIYLLASLIIGVFSNRFIKG
                     ISGYIIAGRSLGTALAVATMTGTELGLITVMYSAQKGFTGGFAAFHIAVICLIVTFFV
                     GLSGFIVARLRESQVMTIPEYYEQRFGRRTRILGGILLAFGGILNMGLFLKVGSMFVV
                     GVTGMDPTGGLLTAVMVTLIALVLAYTILGGMVSVVVTDYFQFVVLSFGMLLMVGLTL
                     NRFGWDQMVDVVFQFKGRGGFDPTVSEGGFGFTYMSWQAVLGLVSCAIWPTAVTRALS
                     SRDVKTVKRQYMLSSLSFMIRFMIPYFLGIGAFVFVMTSGGLYKEIFFPEDPSQAVNN
                     LYAMPLFVSHVVPTGLLGLVTAAMLAAFMSTHDSYLLCWSSVITLDIISPLRHRKPIT
                     DKGKILLTRVIMLLIGAYIVFWGLFYEGSEDIWDYMGITGAIYFTGAISLLLFGLYWK
                     RASSTGAVLSLLGGFSAVLGLGKVRLWLGGLLGMELTPAHVGLFSLFLSMGAMVLGSL
                     LFPDKQRQSVTEAE"
     gene            1685..1828
                     /locus_tag="PKH07_13320"
     CDS             1685..1828
                     /locus_tag="PKH07_13320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35964.1"
                     /translation="MAGWAFLWKVVFVFGVSAFALLSLWVTVFGLADIKRMFEDLGRQ
                     DNQ"
BASE COUNT          358 a          440 c          546 g          581 t
ORIGIN      
        1 tacttgtggg attccctaat gagctcatct ctccagttga cgagtagcga attgtctgct
       61 tgaatgcaag aaatacgctc aaaggaatca tgtctgaagg gaagaagcga ttatgcagct
      121 gaatgaggca gtaaagtccg tctttgcttc tgggaacttc acctctctgg attggggaat
      181 cgttgtcatc tatctccttg cttcactgat cataggggtc ttctcgaatc gcttcatcaa
      241 agggatttcc ggttacatta tcgctgggag aagtctcggt acggcactgg cagtggcgac
      301 aatgaccggg actgagttgg gacttatcac cgtgatgtat tccgctcaaa agggattcac
      361 tggtggtttt gccgccttcc acatcgccgt tatctgtctc attgtgacgt tcttcgttgg
      421 gctctcgggc tttatcgttg cccgtttgcg agagagccag gtcatgacaa ttccagaata
      481 ctatgagcag cgtttcggtc gtcgaactcg gattcttgga ggcattcttc ttgcgtttgg
      541 tggcattctg aacatgggtc tctttttgaa ggttggctcg atgtttgttg tcggcgtgac
      601 aggaatggat ccgacgggag gacttttgac cgcggttatg gtgacactga tcgcgcttgt
      661 gcttgcctat acaattcttg gaggcatggt gtccgttgtc gtaacggact atttccaatt
      721 tgtcgttctg tcatttggga tgctgctcat ggtcggactg acactcaatc gtttcggctg
      781 ggatcagatg gtggacgtcg tctttcagtt caagggtcgg ggtggctttg acccgactgt
      841 ttctgagggt ggattcggat tcacttatat gagctggcag gcggtactcg gtcttgtcag
      901 ttgtgccatc tggccgacgg cagtgacacg agccttgtcg agtcgcgatg tgaagacggt
      961 caagagacag tatatgcttt cctccctgtc attcatgatt cgattcatga ttccctattt
     1021 tctgggaatc ggtgcgtttg tctttgtcat gaccagcgga gggttgtaca aagagatatt
     1081 cttccccgag gatccctccc aggcggtcaa caatctctac gccatgccac tcttcgtgtc
     1141 ccatgtggtg ccgaccggac ttcttggtct tgtgacggcg gccatgttgg cggccttcat
     1201 gtccacccat gacagttacc tcctctgctg gtcgtcggtc atcaccttgg acatcatctc
     1261 gcccttgcgc catcgaaagc cgataacgga taaggggaag attctgttga cgagagtcat
     1321 catgctgttg atcggcgcat acatcgtatt ttggggtctt ttctacgagg gcagtgagga
     1381 tatatgggac tatatgggga tcacaggggc gatctacttc acaggtgcaa tctcgctttt
     1441 gctctttggc ctctattgga agcgagccag ttcaaccggt gccgtgcttt cattgcttgg
     1501 tggtttcagt gcggttctcg gtttgggaaa agtgcggctt tggcttggtg gacttctggg
     1561 aatggaactg acgcccgctc acgtcgggct cttttccttg tttctgagca tgggagcgat
     1621 ggtgttgggt tctctcctgt tcccggacaa acagcgacaa agtgtgacgg aggcggaata
     1681 actgatggct ggttgggcat ttctttggaa ggttgtcttt gtatttggag tatcggcttt
     1741 tgcactgctg agtctctggg tcacggtctt tggccttgcc gacatcaagc ggatgtttga
     1801 agaccttggc aggcaggata atcagtgagg ccgactcggg tctcccagtg agcaagcacc
     1861 gacagtgaga cctgttgttt cgttgtgttc attgctcatt tcttcttgac tcatggtttt
     1921 ccaac
//
LOCUS       DAOQUC010000851         1925 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_77006_length_1925_cov_3.180214, whole genome shotgun sequence.
ACCESSION   DAOQUC010000851 DAOQUC010000000
VERSION     DAOQUC010000851.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1925
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77006_length_1925_cov_3.180214"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            179..1621
                     /gene="gatB"
                     /locus_tag="PKH07_13325"
     CDS             179..1621
                     /gene="gatB"
                     /locus_tag="PKH07_13325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HNT35965.1"
                     /translation="MDFETVIGMEVHTELSTRSKVFCGCSTAFGAPPNSQTCPVCLGL
                     PGVLPVLNRRAFEFGLKAALALDCTIAERVSLDRKNYYYPDLPKNYQISQKYAQIGRE
                     GHLTLSMKDGTTRHVGIWDIHLEEDAGKLIHPEEPQLKGTTLVDLNRAGVPLLEIVSA
                     PDMRTLDEMDCYMHTLRSILRYIDISDCKMQEGSLRFEVNISLRPAGSSEFGGRVEIK
                     NLGSMKAAVKAAAYEEKRQRKVLDKGGTVDQETRLWDEEAGVTRVMRTKEDAEDYRYF
                     PEPDLVEIHVSEDWLSVLREQLPELQDAKRMRFIQQYGLPEYDAAILASDREIADYFE
                     SCCRLHGNPKAFSNWIMVDILRVLDEQDITISELPVSPEHLVGMVRLLDEDKISSKLA
                     KVIFSEMLSTGKHAEAIVQEQGLVQVSDSGEIENLVQQVIDANPGPAREFAEGKDKAI
                     AFLMGQLMKLSKGKANPKMATEIFAKKLRS"
BASE COUNT          455 a          460 c          565 g          445 t
ORIGIN      
        1 gttcagtagt agatatgagc aggttagcac ctgcgggcgc gtgggctatg cccactccct
       61 tggacgcttg ctctgtaaat gaacgcttct tctagactga gtgtcattgg gagtccagaa
      121 atttgcggcg gtgaacatct ggtgagcgat gtccgctatc tgacaacgga gaacagcagt
      181 ggattttgag acagttattg gcatggaagt tcataccgag ttgtccactc gatcgaaggt
      241 gttttgtgga tgcagcacgg catttggggc accgccgaat tcacagacct gtcccgtttg
      301 tctcggcctg cccggcgttc tgccggtgct gaatcgcagg gcttttgagt ttggcctcaa
      361 ggcggctttg gcgcttgact gcaccatcgc agaacgggtc tctctggatc gcaagaatta
      421 ctactacccc gatctgccaa aaaactacca gatctcgcag aaatacgcgc agattggtcg
      481 tgaaggacat ctgacacttt ccatgaagga tggcacaacg cgccatgttg gaatttggga
      541 cattcatctc gaagaagatg cgggcaagtt gattcatcct gaagagcccc agctgaaggg
      601 cacgactttg gtggatctga atcgggccgg agtgccactc ctggagatcg tcagtgcgcc
      661 ggatatgcgt acccttgatg agatggactg ctacatgcac acgctgcgat ccatccttcg
      721 ctatattgat atctccgact gtaagatgca ggaaggctct ttgcgttttg aggtcaacat
      781 ctctctccga ccggcaggca gcagtgagtt tggcggaaga gttgagatca aaaacctcgg
      841 ttcgatgaag gcggcggtga aggctgcggc atacgaggaa aagaggcaac gcaaggtgct
      901 ggacaagggg ggaacagtgg atcaggaaac ccgtctttgg gacgaagagg cgggcgtcac
      961 tcgcgtcatg cgaaccaagg aggatgccga ggactatcgg tattttccgg agccagattt
     1021 ggtggagatt cacgtgtccg aagactggct gtcggtcttg cgcgagcagc ttccggaact
     1081 gcaggatgca aaacggatgc gctttatcca gcagtacggc cttcccgaat acgatgctgc
     1141 gattcttgct tctgaccgag agatcgcgga ctactttgag tcctgttgcc ggctgcatgg
     1201 caatcccaaa gcgttttcca actggatcat ggtggacatt ctgcgagttc ttgatgaaca
     1261 ggacatcacc attagtgaat tgccagtcag tccggagcac cttgttggca tggtacgact
     1321 cttggacgaa gacaagatca gcagcaaact ggccaaggtc atcttttccg aaatgctttc
     1381 gactggcaaa catgccgaag ccatcgttca ggaacaaggt ttggtgcagg tttcagactc
     1441 cggtgagatc gagaatctgg tgcaacaggt tattgatgcc aacccggggc ctgctcggga
     1501 attcgcagag ggcaaggaca aggcaatcgc cttcctgatg ggtcagctga tgaagctgag
     1561 caagggcaaa gccaatccca aaatggcaac agagatcttt gcaaagaagc tgcgttcctg
     1621 atcggagaaa gtaaccttct ctcgctcccc tgaatacagt caacacttga tgaggaaaga
     1681 ggttcgtact gatgtcgctt gctgaatact caggcggtgg agtcagaatc cctcaggaca
     1741 tggaccggtg gactgtctcg gaacaaagaa aggcggcacc agatctgtgc tgggctgtcg
     1801 ttgagtatct tcgatcttgg ccttgagtat ttcgtgacaa agcctggcga gtacatggag
     1861 attttcagcg caggttgtga gagcaggctg caccagtctg gctggttcac ggtctccgaa
     1921 gcatg
//
LOCUS       DAOQUC010000852         1924 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_77092_length_1924_cov_2.292135, whole genome shotgun sequence.
ACCESSION   DAOQUC010000852 DAOQUC010000000
VERSION     DAOQUC010000852.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1924)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1924)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1924
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77092_length_1924_cov_2.292135"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..687
                     /locus_tag="PKH07_13330"
     CDS             <1..687
                     /locus_tag="PKH07_13330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011944096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNT35966.1"
                     /translation="YPLGTDRLGRCLLSRILYGSRISLSIGLVGVTMTLVLGILIGGI
                     SGYYGGLIDTCIQRLIEILRSFPTIPLWLALSAAVPATWSPVQVYFCMTLIIALIGWT
                     GLARVVRGKILALREEDYAVAARVAGVKETSILLRHLVPGFTSHLIVTVTLAIPEMIL
                     AETSLSFLRLGLRPPTISWGVLLQDAQNIRAIALYPWLMAPVFFVIVTVLVFNFMGDG
                     LRDAADPYRN"
     gene            698..1741
                     /locus_tag="PKH07_13335"
     CDS             698..1741
                     /locus_tag="PKH07_13335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015919131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNT35967.1"
                     /translation="MSELLRVHNLHIELSLREGTVKAVNGVNITINRGEVLGLVGESG
                     CGKSITARAILQILPPRAKIAAGQIQLASRDSHSDSLDLATLDPKGDEIRRIRGSDVS
                     MVFQEPMTSLSPVHTVGSQIMEAILLHQNVGRSEARRRAVQVLREVGVPMSDKRLNSY
                     PHELSGGLRQRCVIAMALSCRPRLLIADEPTTALDVTIQAQILELLKQFQRQLGMSII
                     MITHDLGVIAETADRVAVMYLGRIVETAPSEILFGNPHHPYTRGLLASVPRIGRDRDE
                     DLPSIQGRVPHPFETVRGCPFHPRCDRLIPGVCDRDAAPEAIQIAPEHFVACHLYSAE
                     KPRRNKEVLGGMS"
     gene            1738..>1924
                     /locus_tag="PKH07_13340"
     CDS             1738..>1924
                     /locus_tag="PKH07_13340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010677998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNT35968.1"
                     /translation="MSSPLLEVKGLTKHFPVKAGTFKRARGIIKAVDGVSFSIDEGET
                     LGLVGESGCGKTTTGKMV"
BASE COUNT          414 a          535 c          524 g          451 t
ORIGIN      
        1 tatcccctgg gcaccgaccg acttgggcgg tgtcttctca gtcggatcct ctacgggagc
       61 cgcatctccc tttcgatcgg gctcgtcggt gtcaccatga cgctggtgct gggaatactc
      121 atcggaggca tctccggcta ctacggaggc ctcatagaca catgcatcca gcgacttatc
      181 gagattctgc gttcatttcc caccatccca ctttggctgg ctttgtccgc agcagttccc
      241 gcaacatggt cacccgtgca ggtctatttc tgcatgacgc tcatcatcgc acttatcggc
      301 tggacggggc tggcgcgagt cgttcgcggg aagattctgg cactgcgtga ggaagactac
      361 gccgtagccg cacgagttgc gggtgtcaaa gaaacctcga ttcttctacg gcatctcgtt
      421 ccgggtttca cgagtcactt gatcgtcact gtgacattgg caattcctga gatgatcctt
      481 gccgaaacgt ccttgagttt tttgcgtctc ggactgcggc cacccacgat cagttggggc
      541 gttctgcttc aggatgccca gaatatccgt gccattgcac tgtatccgtg gctcatggct
      601 cctgtcttct ttgtcattgt caccgtgctg gtgttcaact tcatgggaga tggtctcaga
      661 gatgcggccg atccctatag aaactagagg aaccgcgttg agcgaactct tgagagtcca
      721 caatcttcac atcgaactgt ccctccgaga aggaaccgtg aaggctgtca atggcgtcaa
      781 cataactatc aacagaggcg aggtgcttgg tttggtcggc gagagtggct gtggcaagag
      841 catcacggca cgggcaatcc ttcagatact tcctccgcgg gcgaagatcg ccgccggcca
      901 aatacaactc gcgtcacgcg actcccattc tgattcgctt gatcttgcga ctctcgatcc
      961 gaaaggggat gagatccgaa ggatccgagg ttcggacgtc tcgatggttt tccaggaacc
     1021 gatgacatcg ttgtcacctg ttcacacagt cggcagtcag attatggaag ccattctgct
     1081 gcatcagaat gtcggccgtt ctgaggcgcg gagacgtgca gttcaagtgc ttcgagaagt
     1141 cggtgttccg atgtcggaca agagactgaa ctcctatcct catgagctgt cgggaggcct
     1201 gaggcagcgt tgtgtaattg caatggctct ttcctgccgt ccgagactgc ttatcgccga
     1261 cgagcccacc actgcgctgg acgtcaccat tcaggcacag attctcgaac tgctcaaaca
     1321 gtttcagcga cagttgggaa tgtccatcat catgatcaca cacgacttgg gagtcatcgc
     1381 ggaaactgcg gacagagttg ccgtcatgta tctcggcagg attgttgaaa cagcaccttc
     1441 tgaaatcctg ttcggcaacc cacatcaccc ctacacacga ggcctgctgg catcggtgcc
     1501 acgaattggc cgagatcgtg acgaagactt gccttcaatt caagggcgtg tcccccatcc
     1561 tttcgagacc gttcgtggat gtccattcca tccgaggtgc gacagactta tccctggcgt
     1621 ctgcgaccga gacgcagccc ccgaagcaat tcagattgca cctgaacatt tcgtagcatg
     1681 ccatctctat tcagccgaga agccgcgccg caacaaagaa gtactggggg gtatgtcatg
     1741 agttctcccc ttctggaagt caagggactg acgaaacact ttcccgtaaa ggcaggaaca
     1801 ttcaaacgcg cacgagggat aatcaaggcg gtggatgggg tctcattctc cattgatgag
     1861 ggggaaacac tgggtttggt cggtgagagc ggctgtggca agaccaccac aggcaaaatg
     1921 gtct
//
LOCUS       DAOQUC010000853         1922 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_77160_length_1922_cov_3.941082, whole genome shotgun sequence.
ACCESSION   DAOQUC010000853 DAOQUC010000000
VERSION     DAOQUC010000853.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1922)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1922)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1922
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77160_length_1922_cov_3.941082"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(927..1028)
                     /locus_tag="PKH07_13345"
                     /pseudo
     CDS             complement(927..1028)
                     /locus_tag="PKH07_13345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003112842.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
     regulatory      complement(1320..1515)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
BASE COUNT          446 a          507 c          498 g          471 t
ORIGIN      
        1 agttggagat cgttggaaag gaagatgcga gaggctgact atgagaaatg ccagtgacac
       61 ccggcaacag cagcaagaca caacaacatc tgcgagaaca cccgatcaac gtggtattcc
      121 ataactttct gacaagacag tttcccggaa cgcgtgtcaa atccttttcc atgacaatgc
      181 agtcagatag actcaccgaa tatctcagtt caaacttctc cgacattttt catatcatga
      241 tcatctgaat gatgcaagga tatgttggtg tgtgctgtca gtgtgtgtga tggagccgat
      301 tgagtggcct ctttgtggga tgcggctgaa aggcagttac cacggtatgt catcgtgctc
      361 tctcctgctg ggggacggtg tggaggggat tgtctctgcc cgtgtgttcg attttcccag
      421 gagcgcgaag aatcccccac accgttcatc caaccggaag tagactatgg tccggttcgg
      481 atgatgtcgc cggttgatga aacaccgttt gttggatcgt aaacgagatg attacatcca
      541 ggagttccag tccaaggttg atcaaagaga gcatccggcc cgacgcatga caaataccac
      601 atcctgcggg aagttgcgtc gtactggagc caagtgttgc tgccccagcc ctgagcgtcc
      661 atccagtcgc aggcggctcg ggtctggtag tagtagatct tcgttggtct gggttctccc
      721 cagggaagcc atcccgcaaa tgccgaatcc ggaaccgaag aaagataggc aaccggtgtc
      781 gtcagaacgg acagcttttc ctcatagccg acgcgcattg catcggggta ggcattccag
      841 tcaacgtgat attgctccag tcccactgcg atggtctgca tgtctgcttc tgcccgcgag
      901 acttttgccc gaatctgcgc ctgcaggaaa ttcggaattg ctatcgcagc cagtatcccg
      961 ataattgcca ctacaataag caactcaatc aaagtgaaac ccgacaaacc tcgcctgaca
     1021 aaacgcatct ctctctcctt ttcgagcctt ccggctcact gtgtttgtga ggcaggctga
     1081 aaaacagagc cgcttacttt gccccaaaga gcgtgtttcg gcttgaaaag gagtgttccc
     1141 gattcccatc gctgtcggat agggcagttg ctcggtcggt gtgaaaccat tccgcagttg
     1201 ctttgatcag gtctgtgcca gaaaacacaa gaaccctcca acgcaaccgc gaagagggtt
     1261 ccagaaaccg tccccagcac aaaggggaca aaccttcacc ttctcattcg cggagaagtt
     1321 ctgtgaagcg cgttgacagc cgtcttctgg cttccgggtc tttctaccgc ccgtcccctt
     1381 cccatcgctg cggggcgaca gtgggtcttc tgatgacggg tttcgtctcc ggttacagcg
     1441 gcgcatccgc gagggattct caccctcttc cgggcgtctc tgagaacgcg agaaaacctc
     1501 gcttgaactc agaaacgcgc taccgccaac gctgcctacc tcattttcaa aacaaatgcc
     1561 acagttggat tgcagtgtca agaacttatt gagagaaaca tactggctca gttaatgggc
     1621 gagggtctgg acttactaca tgcgacacac gtcacttgac actcttttct ctggattata
     1681 cattccaatg tctcctaaga aaccggatct cttggactct ggtttttcgc atcaatgtat
     1741 gtatacggac gcgggtaatg accgcttgtc tgtcggtgtg gatatgctct tgacacaagg
     1801 ttgttcgata ccgtcttagg gaggttgaaa tggcgaatct cgttgtgctt cttgcctatg
     1861 tcgtactgat ggcggtactg gggatctacc tgagccgcta cgtcaagcgt gaggacgact
     1921 tc
//
LOCUS       DAOQUC010000854         1917 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_77484_length_1917_cov_3.705156, whole genome shotgun sequence.
ACCESSION   DAOQUC010000854 DAOQUC010000000
VERSION     DAOQUC010000854.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1917
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77484_length_1917_cov_3.705156"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(27..296)
                     /locus_tag="PKH07_13350"
     CDS             complement(27..296)
                     /locus_tag="PKH07_13350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35969.1"
                     /translation="MSAPDKKCVLLLNPPGVEAYSRDKFCTSISQANYGWPPIDLLVF
                     SGQLREGFEVLVLDAIAERIGFKDALERVQDASPDVIIALTSATS"
     gene            814..1641
                     /locus_tag="PKH07_13355"
     CDS             814..1641
                     /locus_tag="PKH07_13355"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNT35970.1"
                     /translation="MAHDNCLRSYFKAISCNPLLTQAEEVRLARLSRKGNSEARRKLV
                     ESNLLFVAKIAREYLNQGLPMPDLIQEGNLGLMHALEKFDERLGFRLTTYASWWIRLS
                     IKRAVEQKSRQIRIPSNKLELLRKMRAFQSCYEKMHGQSPSVEVIAKEFKIPKTKIER
                     VIGLDTHVTSFDLPTTEDGVAYELLLSSEDSVLPSETIHMEQMREKLERAMEVLSVKE
                     RKVLTMRYGLQDEGSSASLREVGKQMGMSPEGVRRIENKALSKLRRPHVRAYVENFV"
     gene            complement(1714..>1917)
                     /locus_tag="PKH07_13360"
     CDS             complement(1714..>1917)
                     /locus_tag="PKH07_13360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013256454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HNT35971.1"
                     /translation="VVHFEKANPAIDGLYQVINQWFCQNALRGLTWVNREQDLGITGL
                     RQAKMSYHPDHFVKKYKVCPKSD"
BASE COUNT          512 a          467 c          510 g          428 t
ORIGIN      
        1 tgtcgcagga aggcgaagtc tttcggtcag gacgttgcgg acgtcaaggc gatgatgaca
       61 tcgggagaag catcctgaac tcgttcgagt gcgtccttga acccaatgcg ttccgcgatg
      121 gcgtccagaa ctaaaacctc aaagccttca cgcagctggc cactgaatac cagcagatca
      181 atgggaggcc agccgtaatt cgcctgagag atagaggtac agaacttgtc ccgactgtaa
      241 gcttctactc cggggggatt gagaagtaga acacacttct tgtcaggggc actcatgcca
      301 tcgtctccca aggaaaccaa actccgcaag aggtggtttc tttcccgagc aagcaccgca
      361 caaagaacgt cagcgcactg caactgaaga cacagagtcg tctccgtatc catacctgag
      421 gacattacca gcaagaaacc aagatgtaaa tgtcggcata gccgctgttc tgtgacgcaa
      481 acccaaggat accccgccta ctcgtccgca gttccgtagc ttacatcgtc gttggtgcta
      541 aagtcagtcc tctcttccca ctctatcaag aaaagcaaga gtgagagaaa attctcacag
      601 gtgggaaata tcgaacacgg ataagtcgta cagtccgtag ctgaaaggat tcgatgatcg
      661 atcaaaatca tgcgcgctat ctcgttcaga attaaccgct tcagacttag gattccgacc
      721 ggaattcatg tttggtatgg atattgcttt cagcatagtt agtggattgg aaggctctcg
      781 atggagagca tatttggatg gagggacaag atattggcac acgataattg tcttcgatcc
      841 tatttcaaag cgatctcgtg taaccctctg ctgacgcagg cagaggaggt acggttggcg
      901 cgactttctc gaaaaggtaa ctccgaagca cgaagaaagc tggtggagtc gaatctgctg
      961 tttgtggcga agattgcccg agagtacttg aaccagggac ttccgatgcc ggatctgatt
     1021 caggagggca atctcggcct gatgcatgct ttggagaagt ttgatgagcg cttgggattc
     1081 cgtctcacga cctacgccag ttggtggatt cgactgagca tcaagcgagc ggttgaacag
     1141 aagagccgtc agataaggat tccatcgaac aaacttgagt tgctcaggaa gatgagggct
     1201 ttccagagtt gctacgagaa gatgcacggt cagagtccga gcgttgaggt gattgcaaaa
     1261 gagttcaaga tccccaaaac aaaaatcgag cgtgtgatcg gacttgatac gcatgtaact
     1321 tcgtttgacc ttccgacgac cgaggatgga gtggcctacg aactgctgct ttcctcagag
     1381 gattctgtcc tgcccagtga gacgatccac atggagcaaa tgcgggaaaa acttgaacgt
     1441 gccatggaag tgctgtcagt taaggaacgc aaggtactca cgatgcgcta tggacttcag
     1501 gatgagggat caagcgccag cctgcgcgaa gtaggcaagc agatggggat gagtcctgaa
     1561 ggagtcagac gcatcgagaa caaggcgctt tcaaaacttc gtagacctca cgttcgggca
     1621 tacgtcgaga acttcgtctg agcgcaggga gattccgcac tcatctctgc cagggagtgg
     1681 acatagccca cacacaccca agtgttggcc tgctcagtct gacttgggac acactttgta
     1741 cttcttcaca aaatgatcag gatgataact catcttcgcc tgccttaaac ccgtgatccc
     1801 cagatcctgc tcgcggttga cccaggtcag acctctcaag gcgttctggc aaaaccactg
     1861 attgatgact tggtacaagc catcaatggc gggattggct ttttcaaaat gaaccac
//
LOCUS       DAOQUC010000855         1914 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_77741_length_1914_cov_2.514793, whole genome shotgun sequence.
ACCESSION   DAOQUC010000855 DAOQUC010000000
VERSION     DAOQUC010000855.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1914
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77741_length_1914_cov_2.514793"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1914)
                     /locus_tag="PKH07_13365"
     CDS             complement(<1..>1914)
                     /locus_tag="PKH07_13365"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HNT35972.1"
                     /translation="EMNTVSPESLIRNLLVGKRMADDFGGAMKTGYTATSYGQISQLP
                     QIYAGFDLNCAMTYRGTNKHQIPPICLWEGADGTCIAHIRCFDEVTRTNWFFFLHYEL
                     VLRKSPRDLRIKYDPKNLPVHMADDTLYQVAFQLKHEDMDFEHDPDKVRAAVRHFAKQ
                     AEPQAIANHLLALDMEDNAVPYVNLPKLIAAVNAVQNDYEARQTTLDEYVQTALTSVD
                     RKSLPVLKGEWRYAAIEAGFNGLLGATHSSRIPLKLLNEEAETELISVAEPLSTFSAL
                     MGGQYWRSLLDRAWLKLLKCHPHDSICGAAIDEAHDDMPSRFRDVRMLARETSRKACE
                     DIWEKLDTAASFEQGDLTITFFNSLPFDRKGVVPAVIDTPVTDFGDIYIEPCSGAGPI
                     LEGIDVDELITYNYFDVVDEKGVRIPYKVLEKEDLTIEAERKLDSNAIVYNLERHRML
                     LDVNIPALGYRTYALRPRKREYVTDPVPGSDRAHIAQPHGVLENEFLRIQIQPNGTFD
                     MMDKKAGKQFRGMHFFADDGSVGNAHMQKSPLRDSTITSLGSHATLTLVESNALRGTW
                     RIDLSMSLPAEADLDARNRSRRAVTLPITTWLTLSKDARRLEIRTRFENQARDHRLRV
                     LFPTDIQTGTVDVE"
BASE COUNT          413 a          517 c          524 g          460 t
ORIGIN      
        1 ctcaacgtca accgttccag tctgaatgtc cgtcgggaag agcacccgaa gtcggtgatc
       61 gcgtgcctga ttctcaaaac gtgtcctgat ctcaagacgc ctggcgtcct tgctcagggt
      121 cagccatgtt gttatcggaa gcgtgacggc cctgcgagac cggttcctgg cgtcgagatc
      181 ggcttctgcg gggagactca tgctgagatc aatgcgccac gtgcctcgaa gcgcgttgct
      241 ttccaccaga gtcaaagtgg cgtgggagcc cagactcgtg atcgtactgt cgcgcagcgg
      301 agacttttgc atgtgggcgt tgccgacact gccgtcgtcc gcgaaaaagt gcattccgcg
      361 gaactgcttt ccagccttct tgtccatcat atcgaaggtg ccattgggct gaatctgaat
      421 tcgcagaaac tcgttttcca gcacgccgtg tggctgggcg atgtgcgccc gatcacttcc
      481 tggaaccgga tccgtgacat actcacgctt tcgcggacga agtgcgtagg tacgatatcc
      541 cagcgccggg atattcacgt caaggagcat ccgatgtctc tccaggttgt agacaatggc
      601 attggaatcg agtttgcgct ccgcttcgat ggtcaggtct tctttctcga gaaccttgta
      661 cgggattctg acgcccttct cgtcaacgac gtcgaagtag ttgtacgtga tcagttcgtc
      721 aacatctata ccttccagta tcggacccgc accgctgcac ggttcaatgt agatatcgcc
      781 aaaatccgtc acgggcgtgt caatgactgc aggcacaacg ccttttcggt cgaagggaag
      841 ggaattgaag aaggtgatcg tcagatcgcc ttgctcaaag gacgccgccg tatcgagctt
      901 ttcccagata tcctcgcagg ctttgcggct ggtctctcgc gccagcatcc tcacatctcg
      961 aaatcgggaa ggcatgtcat catgtgcctc atcaatggca gcgccgcaga tgctgtcgtg
     1021 gggatggcac ttcagaagct tcagccatgc cctgtccaga agagaacgcc agtactgccc
     1081 acccatcaac gccgagaacg tggacaaggg ctctgctaca gagatcaact ctgtctccgc
     1141 ttcttcgttc agaagcttca gtggaatccg tgaagaatgg gtcgctccca aaagcccgtt
     1201 aaaacccgcc tcaatggccg cgtagcgcca ttctcccttg agcacgggaa gactctttcg
     1261 gtcaacagag gtcaaggctg tctggacgta ttcatcgagc gttgtctgtc tggcctcgta
     1321 gtcattttga actgcattga cagcagcgat gagtttcgga agattcacat agggaactgc
     1381 gttgtcttcc atatccaagg caagaagatg attggcgatg gcttgtggct cagcctgttt
     1441 ggcgaaatgc ctgaccgcag cgcgcacctt gtcgggatca tgttcgaagt ccatgtcctc
     1501 atgtttcagc tgaaaggcaa cctgatacag cgtgtcatct gccatgtgaa ctggaagatt
     1561 ctttgggtcg tactttatcc ggagatcgcg gggagacttc ctgagaacca gttcgtagtg
     1621 cagaaagaaa aaccaattgg tgcgcgtgac ttcgtcgaaa caccggatgt gtgcgatgca
     1681 ggttccgtca gcgccttccc aaaggcagat cggcgggatc tggtgcttat tggtgcctcg
     1741 ataggtcatg gcgcagttca ggtcaaagcc agcatagatc tgtggtaact gggatatctg
     1801 accgtaggac gttgcagtgt aaccggtctt cattgctcca ccgaaatcat ctgccatcct
     1861 tttgccaacg agcagattcc gaatcaggct ttcgggagaa acggtattca tctc
//
LOCUS       DAOQUC010000856         1914 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_77743_length_1914_cov_2.500269, whole genome shotgun sequence.
ACCESSION   DAOQUC010000856 DAOQUC010000000
VERSION     DAOQUC010000856.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1914
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77743_length_1914_cov_2.500269"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            117..944
                     /locus_tag="PKH07_13370"
     CDS             117..944
                     /locus_tag="PKH07_13370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35973.1"
                     /translation="MKTLSVPASYYQQFDADFSLGVPAEGYGGWKTEDIEISLEHTAV
                     VVMHAWDFGSREDYPGWHRAVEYISRAQQICRDVFPPLLSAVRASGLPLLHVVSSGHY
                     YQNYLGYKRAVELAGAPPTALERIPQDDTYRRLCEFRSEKVFVGAHNAADVKRGFANL
                     DFAREARPLGDEGIAENGHHLFALCRDAGINHLIYAGFAINWCLLLSPGGMAEMSARG
                     FLCSVFRQAVTAVENKDTARSELCKEIGLWRTALAYGFVFDVEPFIRACESLACRAG"
     gene            1067..>1914
                     /locus_tag="PKH07_13375"
     CDS             1067..>1914
                     /locus_tag="PKH07_13375"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35974.1"
                     /translation="MVGRATAVGGLPLEFFERAVDVKKNAFPLLMLVLMSSVSVVYAA
                     PGVPSTIKWVYVLGHTHLDIGFTEPPETVAEKQKTMTDGQISYAQSRPTYKWNIETTW
                     TLEQWIKRSSPSDIFSFRYMVLGDRFGVAGSHSTMHSPKLGFEQVVRLLWNAQRYRNL
                     YGFNVETVFHDDVPGVAWTYPQILARSGIKYLVCGENLFIGGGFTQPYDSYPFYWKGP
                     DGSRVLTWSARNSYTEGFSNYGLPWISNVPVDQAKLETALTTLTNQGYPYDAVVVQQA
                     FDNGVVG"
BASE COUNT          416 a          493 c          544 g          461 t
ORIGIN      
        1 atgcccactc cctcagacgc tcgatcaata aatgacacgc ccctctcgga ctccgtgtca
       61 ctaggagaat acgagtctct ctggcagaga acaagcggtc aaggatgttg atcaacatga
      121 aaacgctctc cgtgccggcc agttactatc agcagtttga cgcggacttc tcgctgggcg
      181 ttccggccga aggctatggc ggatggaaaa ccgaggacat cgaaatatcc ttggagcaca
      241 cagcagtggt cgtgatgcat gcctgggact ttggctcacg ggaggactat ccaggttggc
      301 atcgagccgt ggagtatatt tcccgcgctc agcagatctg cagagacgtc ttcccacctc
      361 ttctctctgc cgttcgggca agtggcttgc cgctgcttca tgtagtcagt tctggtcatt
      421 actaccagaa ctacctcggc tacaagcgag cagtcgaatt ggcaggagcg ccgccaactg
      481 ccttggagag aataccgcag gacgacacat acaggaggct ttgtgagttt cgctcggaaa
      541 aggtctttgt cggtgctcat aacgcagcgg atgtgaaacg aggttttgca aacctcgact
      601 ttgcccgaga ggcgcgcccc ctgggtgatg aagggatcgc cgagaatggc catcacctgt
      661 ttgcactttg ccgcgatgcc ggaatcaacc atctgatcta tgcagggttt gcgatcaact
      721 ggtgcttgct tttgtccccg ggcggaatgg cggagatgag cgcgcgagga tttctctgtt
      781 ccgtctttcg tcaggcggtg accgcagttg aaaataagga caccgcacga tccgaattgt
      841 gcaaggagat tggtctctgg cgcactgctt tggcttatgg ttttgtgttt gacgtggagc
      901 cattcatccg cgcgtgcgag agtctggctt gccgagcagg ttagcacctg cgggcgcgtg
      961 ggctatgccc actctcttgt tctgaactga gtgacaacat gagaatgaac tgacacaaaa
     1021 gtctctcttc tgctacctta tgcctggttg cggagagaaa gatggcgtgg taggtcgggc
     1081 gactgcagtc ggaggcttgc cacttgaatt cttcgagagg gcagttgatg tgaagaaaaa
     1141 tgcatttccc ttgttgatgc ttgtcttgat gtcgagtgtt tcggtggtat acgccgcgcc
     1201 tggagtacct tcgaccatca agtgggtcta tgttcttgga catacgcatc tggatattgg
     1261 tttcacggag ccgccggaga cggtggccga aaaacaaaaa acgatgactg atgggcagat
     1321 tagctatgca cagtctcgtc ccacctataa gtggaacatt gaaacgactt ggacgctgga
     1381 gcaatggatc aagcgaagca gcccttccga catcttttcc ttccgataca tggtgcttgg
     1441 tgatcgcttt ggcgtcgcag gcagccactc caccatgcac agcccgaaac tcggcttcga
     1501 gcaagtggtg cgacttttgt ggaatgcgca acgctacaga aatctctacg gctttaacgt
     1561 ggagacagtc tttcacgacg acgtgcctgg cgtcgcatgg acatatcctc agattcttgc
     1621 ccgttcggga atcaagtatc tggtctgcgg tgagaatctt ttcatcggtg gcggattcac
     1681 tcagccctat gactcgtacc cgttctactg gaagggtccc gatggcagcc gggtgctgac
     1741 atggtcggct cgcaacagct acaccgaagg cttttcgaac tacgggcttc cgtggatctc
     1801 caatgtacct gtggatcagg caaagctgga aacggctctg acaactctga caaatcaggg
     1861 ttatccctac gacgccgttg tcgtgcagca agcttttgac aacggcgtcg tagg
//
LOCUS       DAOQUC010000857         1914 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_77753_length_1914_cov_2.250672, whole genome shotgun sequence.
ACCESSION   DAOQUC010000857 DAOQUC010000000
VERSION     DAOQUC010000857.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1914
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77753_length_1914_cov_2.250672"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..689)
                     /locus_tag="PKH07_13380"
     CDS             complement(<1..689)
                     /locus_tag="PKH07_13380"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: protein motif:HMM:NF002537.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HNT35975.1"
                     /translation="MITQQETLTLEQLESSTAEELLGWAFAEYGSRAGIITSFQDTGC
                     AIIDMAQRAAPGLRVITIDTLRLHEETYRLIESIEARYGIVVERFFPDPQRLDQMIED
                     YGEYLFFDGREGQQRCCFVRKVEPNQRALDTLDVWFTGLRRDQSCHRSNTSKVSLVTH
                     GDREILKIAPLADWSEERVRSYLDEHDVPINALYDKGYTSIGCTICTTPTRVCEDKRS
                     GRWRWFGCRED"
     gene            complement(777..1688)
                     /locus_tag="PKH07_13385"
     CDS             complement(777..1688)
                     /locus_tag="PKH07_13385"
                     /inference="COORDINATES: protein motif:HMM:NF013259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNT35976.1"
                     /translation="MAESAPCDIKSRGFIPMKEPGLYSVRIHVVGGFMRSDWLAEVSR
                     LAEKYGRGHVHLTTRQGVEIPYVPQENIYALQDELAESGLRVGGCGPRVRTMTACQGL
                     GCRHGLIDAQGIAQRLMIRVEHRVLPHKFKVCIAGCPNACPKPVENDFGVQGVIQKTF
                     HREECKACGLCVKVCPSVGALRIEDKSLALCEESCIGCGKCIQACPFGALESERTLYQ
                     VSLGGKMGRFPKPAYQLPFLLPSESAVGQVLDATLDWYCENGRSKERFGDALERIGVE
                     FLIPILAPVAKQESPAYQCTGPMRCSP"
BASE COUNT          436 a          558 c          465 g          455 t
ORIGIN      
        1 tcgtcttctc gacaaccgaa ccagcgccat ctgcccgacc ttttgtcctc gcagacgcgc
       61 gttggcgtgg tgcagatggt gcagccgata ctcgtatacc ctttgtcata caacgcattg
      121 atgggaacgt cgtgctcgtc cagatacgac ctgacgcgtt cttccgacca gtccgcaagt
      181 ggagcgatct tgagaatctc tcgatcaccg tgagtcacca atgagacctt gctggtgttt
      241 gagcggtgac atgactgatc gcgcctcaga ccggtgaacc agacgtccaa cgtatccagc
      301 gccctctggt tgggttcgac ctttctcaca aagcagcacc gttgctgccc ttctcgtcca
      361 tcgaagaaca ggtactcgcc atagtcttcg atcatctgat ccaggcgctg tgggtcaggg
      421 aaaaaccgtt cgaccacgat gccatatcgt gcttcaatgc tttcgattag acggtatgtt
      481 tcttcgtgca atctcaacgt gtcaatcgta atcactcgaa gtccaggggc agccctttgt
      541 gccatatcaa tgatggcaca accggtgtcc tgaaaactcg tgatgattcc tgcccgagaa
      601 ccgtattcag caaatgccca acccagaagt tcctctgcgg tggaagattc caactgctcc
      661 aatgtcaaag tctcttgctg tgtaatcatt gttactgtgc tccttgatcc gaaaagaggc
      721 gacgtctatg cctctcactc atcgatctgc ggaaagtcaa gtctccagag ttcgcttcat
      781 ggggagcaac gcatgggacc tgtgcattga taggcgggag actcctgctt tgccacgggt
      841 gcaagaatgg gaatcaagaa ttcgacaccg atccgttcca gtgcgtcacc gaagcgctcc
      901 ttcgagcgcc cgttctcgca gtaccagtcc agagtagcat ccaatacttg tcccacagca
      961 ctttctgagg gcagcaggaa aggcagctgg taggcaggct tgggaaatcg tcccatcttg
     1021 cctccaagag acacttgata gagcgtcctt tcggactcga gcgctccaaa gggacaggcc
     1081 tgaatgcact tgccacagcc gatgcaggat tcttcacaga gcgcaaggct cttgtcttca
     1141 atccgaagtg caccaactga gggacagacc ttgacacaca agccacaagc cttgcattcc
     1201 tcacggtgaa atgtcttctg aatgactccc tgcactccaa agtcattctc cacaggcttg
     1261 ggacatgcgt ttgggcaacc ggcaatgcac actttgaatt tgtgtggcaa tactcgatgc
     1321 tccacacgta tcataagcct ctgggcaata ccctgagcgt caatgagccc atgtcggcaa
     1381 cccagtcctt gacaagccgt catggttcga accctggggc cacatccccc aacacgaagc
     1441 cccgactcgg ccaactcgtc ttgaagagcg tagatattct cttgcgggac atacgggatc
     1501 tcgacccctt gtctggtggt tagatgaaca tggccacggc cgtatttctc agccagtcgt
     1561 gacacctcag caagccaatc ggagcgcatg aatccaccca ccacatggat acgaacagag
     1621 taaagacccg gctccttcat cggaatgaag cctctcgatt tgatgtcgca gggcgcgctt
     1681 tccgccatct gccaccacct ctctttcgtt tctcttcaga tcccaaccgc atctttcgtg
     1741 ggcagaagtt ctatattatc ctgttttcct atcgaactac tatgaaaata gcacgttctt
     1801 cccctttgtc aacaagaatc tctccggaac aaggggtgtg aggtggtcgg tcggcgtcta
     1861 agccatgttt cttcagctag acccagttgt tgcgcgggat agcagggcgg ctca
//
LOCUS       DAOQUC010000858         1913 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_77802_length_1913_cov_2.815393, whole genome shotgun sequence.
ACCESSION   DAOQUC010000858 DAOQUC010000000
VERSION     DAOQUC010000858.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77802_length_1913_cov_2.815393"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..721)
                     /locus_tag="PKH07_13390"
     CDS             complement(<1..721)
                     /locus_tag="PKH07_13390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35977.1"
                     /translation="MNKPRESDSPAVHSDRLPLSQTSSLTHQQDLEFLYRLECRAILP
                     LKWTIFFLCVLLAFAFTPGYSPSFPVFVLLVFYGCSTLFLTYMIAFKRCPLTCIRGLS
                     YISFGIDVLAMTALVYLTGGLRSDFYILFFLVVLRGVGYFPTARQNFIVDIVISILFV
                     LALYLGQPDAETLLNRTFLVRMSLLCGAVLLSWYLMRIQVSERQNTELANARLLLQTE
                     YNRNMLESMTNGVVAMDSAGNI"
     gene            complement(746..1363)
                     /locus_tag="PKH07_13395"
     CDS             complement(746..1363)
                     /locus_tag="PKH07_13395"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNT35978.1"
                     /translation="MATNCYFLVCEPDNIGVVIDPGGDAEHILEAVERAGVERAVILN
                     THGHGDHIEANEEISLSLDAPIYIGAGDAPALTDPAVNLSVFLGTPIVSPAATRLLKD
                     GDILRFGSETLEVMETPGHSPGSVCFYIRRLGWLFCGDLVFLESVGRTDLPGGDFPIL
                     LQSIAERVLTLPEETVLYPGHGPQTTVGHEVKHNSFLGDLSRFHM"
     gene            complement(1251..>1913)
                     /locus_tag="PKH07_13400"
     CDS             complement(1251..>1913)
                     /locus_tag="PKH07_13400"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HNT35979.1"
                     /translation="CLISAAVFGMTLGFLRFNFHPARIFMGDMGSLTLGFVLAAIGVM
                     GDRKSSTALPLLIPLIALGVAILDTAMAIVRRLGRGAHVFQADREHLHHRLMALGLSH
                     RQTVLLIYYFCAYLSVSALVLSGLATRQTVLVLIVLAMGLFLAMMALEFIERKAKTVS
                     SVQLPCSTLGSHIHENGNTGGRTAGNKLLFPRLRTGQYRGRHRPGRRRGAYLGSSRTR
                     RR"
BASE COUNT          518 a          510 c          524 g          361 t
ORIGIN      
        1 tgatgttgcc agcgctgtcc atcgcaacga ctccgttggt catgctctca agcatgttgc
       61 ggttgtactc ggtctgaagc aggagtcgcg cgttggccaa ctcggtgttc tgacgttcgg
      121 aaacctggat gcgcatgaga taccagctca gcagcaccgc accgcacaga aggctcatgc
      181 ggacaagaaa ggttctgttc aggagcgttt cggcatccgg ttgtcccagg tagagcgcca
      241 gaacgaacag gatggagatg acaatgtcaa cgatgaagtt ctgcctcgcg gttggaaagt
      301 agcccacccc acgcagcacc acgagaaaaa acagaatgta aaagtcactt ctgagtccac
      361 ccgtcaggta gaccaaagcc gtcatggcca acacatcaat accgaaggag atgtagctga
      421 gcccgcgaat acaggtcaga ggacaacgct taaacgcgat catgtacgtc aggaaaagtg
      481 tgctgcatcc gtagaaaacc aaaagcacaa acacagggaa acttggcgaa tacccaggtg
      541 tgaaagcaaa agcaagcaga acgcagagga agaagatcgt ccatttgagg ggcaagatgg
      601 ctcgacactc caaacggtag agaaactcca gatcttgttg gtgcgtcaga gaactggttt
      661 ggctcaaagg caaccgatcg gaatgcacgg ctggagaatc tgattctctt ggcttgttca
      721 tcggcatgcc accacccttc cgggactaca tgtggaaccg tgacagatcc ccaagaaagg
      781 agttgtgttt cacctcgtgt ccaacggtag tctgcgggcc gtgtcctgga tagagaaccg
      841 tctcttcggg taaagtgagt acgcgctctg cgatggattg aagcaatatg ggaaagtcgc
      901 caccgggaag atccgtgcgt ccaacacttt ccagaaagac cagatcccca cagaagagcc
      961 atccgagcct gcgaatgtag aagcaaacac ttcccggtga gtgccctgga gtttccatga
     1021 cttccagagt ctcagaaccg aagcgcagaa tatcgccgtc cttgagaagt ctggtggctg
     1081 caggtgagac aatgggtgtt ccaaggaaga cagacagatt cacggcagga tctgtcaaag
     1141 caggagcgtc cccagcgccg atgtagatcg gggcatcaag ggagagggat atctcctcat
     1201 tggcctcgat gtgatcgcca tggccgtgtg tattgaggat gaccgctcgc tcaacgcctg
     1261 cgcgttcgac tgcttccaag atatgctccg cgtcgccgcc cgggtctatg acgaccccga
     1321 tattgtccgg ttcgcagacg aggaaatagc agtttgttgc cagcggtccg accaccagtg
     1381 tttccatttt catggatgtg actcccaagt gtcgagcaag gcagttggac ggaagacact
     1441 gtctttgcct ttcgctcgat gaactccaat gccatcatgg caagaaaaag ccccatcgcc
     1501 agaacgatga gaacaagcac ggtctggcgc gttgccagtc ctgacagaac caaagctgag
     1561 acggaaagat aagcgcaaaa atagtagatc aacagcactg tctgccgatg cgagagtccg
     1621 agagccatca gacgatgatg caaatgctcc ctgtccgcct gaaagacatg ggcacctcga
     1681 cccagccgac gaacgatggc cattgcagta tccagaatgg ctacccccaa ggcgatcaac
     1741 ggaatcagca gtggcaacgc agtggagctt tttcgatccc ccataacacc aatggcggcc
     1801 agcacgaagc ccagtgtcag acttcccata tcacccatga agatgcgcgc agggtggaaa
     1861 ttgaaccgca gaaatccgag agtcatccca aagaccgcag cgctgatcag aca
//
LOCUS       DAOQUC010000859         1908 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_78168_length_1908_cov_2.545062, whole genome shotgun sequence.
ACCESSION   DAOQUC010000859 DAOQUC010000000
VERSION     DAOQUC010000859.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1908
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78168_length_1908_cov_2.545062"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..281
                     /locus_tag="PKH07_13405"
     CDS             <1..281
                     /locus_tag="PKH07_13405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35980.1"
                     /translation="RKVTDNIYMIKIDRPRQCSIRLKRPHPSGMRLRKKDSDDYILWR
                     PTPQQMGTHQVTIVFSGPETSEKEVTAYVGTHEVLKALREAQAAPPQH"
     gene            618..1646
                     /locus_tag="PKH07_13410"
     CDS             618..1646
                     /locus_tag="PKH07_13410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35981.1"
                     /translation="MNRRTVFVLGAGASCPYGYPSGARLRELICYEDGFIKHYREYAK
                     ADSHKQAVIRDVEQFRDTFAKSHIKSIDLFMSKNPQLAPIGKRIIAFEILRSEKDSRF
                     AEDAQWRHEQLQELRSHDRADVRARYRGVADCIGGDWCSFLYNRLIEGQTETDPLPDL
                     TGDQLAFITFNYDRSLEQFFYESLRSSFTEVPEDRIVESLRNLKIIHIYGQLMPLEWQ
                     NPQTGVDYRPQQIDESLLQNAAANIRTIYEQEAGPDVEEARRVLSEASQILILGFGYA
                     RENMAILDLPTTISSDCQVYGTAYGLIGEERQRAHASLHNPRKIPQERTVMESGDVDC
                     LMLLRKRF"
     gene            1647..>1908
                     /locus_tag="PKH07_13415"
     CDS             1647..>1908
                     /locus_tag="PKH07_13415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35982.1"
                     /translation="MENNDSGDVYEQAVIKTVAGFFRGSPKHLFGVPRFPCGRLFIVA
                     AAGHRGSRFEDERPVIRARPVVAGGREISRTAVRKRRPGDRGA"
BASE COUNT          481 a          522 c          509 g          396 t
ORIGIN      
        1 atcgaaaagt cacggacaac atctacatga tcaagattga ccgcccgcgg cagtgcagta
       61 tccgcctgaa gagacctcat ccttcgggaa tgagactgcg aaagaaagac agcgacgatt
      121 acatcctgtg gagacccacg ccgcaacaga tgggcacgca tcaggtgacg attgttttca
      181 gcggcccgga gacttctgaa aaagaggtca cggcctatgt cggcacccac gaggttctca
      241 aagccctgcg agaggcccaa gcagccccac cccaacactg acgacggaaa tatcaccgtc
      301 accctctccg ccgccccgct cacgcctccg tctctcacgc cagccctcca agcaacatga
      361 gccaaacccc gccggcagta agtaaggcgt ccccagaatt tatggcgtcc ccagaattct
      421 gccacttatc gccggcgcat tcaagatgcg gcttgacctg cattccccac gacggcataa
      481 tccactttgg ttggtccagc ccgaagccgc cccgcgagaa gccgagggcg gcgtcaaacg
      541 cccactgcaa ggctatggca agtacgggtg atgcaccact gatattgact catttgccaa
      601 tgggtgtgaa ggtggcaatg aacagaagaa cagtctttgt tcttggtgca ggagcgagtt
      661 gcccctatgg ctacccatcg ggggctcggc tccgggagtt gatatgctat gaagacggct
      721 tcatcaaaca ctatcgggaa tacgcgaagg cggattcaca taagcaggca gtcatacgag
      781 acgttgagca attcagagac actttcgcca agtcacacat caagtcaata gatctcttca
      841 tgtcgaagaa tccgcaactc gcacccattg gaaaacgcat catcgcattt gagattctga
      901 ggtcggagaa ggacagccgt tttgccgaag atgcgcagtg gcgacatgaa caacttcagg
      961 aactccgttc ccatgacaga gcggacgtca gggcacgcta tagaggggtg gcggattgca
     1021 ttggtggcga ttggtgctca ttcttgtata acaggctgat tgaaggacaa actgagacag
     1081 atcctctacc agatcttacc ggtgaccagc tagcattcat cacatttaat tatgacagat
     1141 cgctggaaca gttcttctat gaaagcctcc ggagttcctt cacagaagtt ccggaggatc
     1201 gtatcgtcga gtcactgagg aatctcaaga tcatccacat ctatggtcag ttgatgcctt
     1261 tggaatggca gaatccccag acgggcgtgg attatagacc tcagcagatt gacgagtcgc
     1321 ttctacagaa tgctgccgcc aacatacgaa cgatatatga acaagaggca ggtccggatg
     1381 tagaggaagc acgacgcgtt ctctctgagg catcgcaaat cctcattctc ggcttcggct
     1441 atgcccgtga gaatatggcc atactcgatc tgcctacaac gatatcttct gattgccaag
     1501 tgtacggtac ggcgtacggc ctgataggag aagagaggca gcgagcacat gcctctcttc
     1561 acaaccccag gaagattcct caagaaagaa ccgtcatgga atcaggcgat gtcgattgtc
     1621 tcatgttgct gaggaagcgc ttctgaatgg aaaacaatga ttccggggac gtctatgagc
     1681 aagccgttat caagacggtc gcgggatttt ttcgcgggag tcccaaacat ctattcggcg
     1741 tccctcgatt cccgtgcggg cgactgttca tcgtcgctgc ggcggggcat cgaggtagcc
     1801 gtttcgagga cgagcgccca gtcattcgtg cccgtcccgt tgtcgccggg ggtcgtgaaa
     1861 tttcgagaac cgctgttcgc aaaaggagac ccggcgatcg aggcgcat
//
LOCUS       DAOQUC010000860         1905 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_78359_length_1905_cov_2.771351, whole genome shotgun sequence.
ACCESSION   DAOQUC010000860 DAOQUC010000000
VERSION     DAOQUC010000860.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1905
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78359_length_1905_cov_2.771351"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..347)
                     /locus_tag="PKH07_13420"
     CDS             complement(<1..347)
                     /locus_tag="PKH07_13420"
                     /inference="COORDINATES: protein motif:HMM:NF037644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HNT35983.1"
                     /translation="MAGLTLGEALQKSANFFDQRGCESPRLSAELLLAHVLQTDRLHL
                     YMEYSRPLTERETDAYREMVRRRGQGEPVAYLLGRKEFFSLDFEVDSSVLIPRPETEV
                     LVQVALEVLNIRES"
     gene            complement(387..1460)
                     /gene="prfA"
                     /locus_tag="PKH07_13425"
     CDS             complement(387..1460)
                     /gene="prfA"
                     /locus_tag="PKH07_13425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HNT35984.1"
                     /translation="MLERVRKIRERLAEIDQEMSNPDVFRDNNRIQSLGKERSELAPI
                     VVAADQLDQLLGDLAEAESLLKSEDEETREYAESEKAILQAQVTEAQAHLEDLLLPKE
                     AVDDRNSIIEIRAGTGGEEAALFASDLLRMYMRYVERHGWKAEMLSSNPTGIGGFKEV
                     IFAIRAKGAYSFLKFEGGVHRVQRVPATEASGRIHTSTATVAVLPEAEEVDVNLKEDD
                     LRIDRFCSSGPGGQSVNTTYSAVRLTHIPTGLVVSCQDERSQIKNLAKAKMVLRSRLL
                     EAEMQAQREQERRDRGLQVRTGQRNEKIRTYNFPQSRLTDHRIGFTSYRLPEIMDGDL
                     DELITALNVADRAERLATGKKDA"
     gene            1719..>1905
                     /locus_tag="PKH07_13430"
     CDS             1719..>1905
                     /locus_tag="PKH07_13430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35985.1"
                     /translation="MMMNFSRVIWVFGCLLLILPLSQGLADPAPSVRWSGSKNLSELE
                     YNAIAPTILADANGLHIL"
BASE COUNT          414 a          568 c          456 g          467 t
ORIGIN      
        1 gactccctga tattcaggac ttccagtgcc acctgaacca gcacttctgt ttccggcctc
       61 ggaatgagca cggaggaatc cacttcaaaa tcgagggaga agaactcctt gcgacccaga
      121 aggtatgcca ccggctcccc ctgtcccctt ctccttacca tctcgcggta ggcatcggtt
      181 tcccgttcgg tcagaggacg gctgtattcc atatatagat gaagccgatc cgtctgcaat
      241 acatgtgcca gaagcaattc cgccgacaac cgaggtgact cacaccctct ctggtcgaaa
      301 aagttcgccg atttctgcag cgcctcaccc aaagtcagtc ccgccatgtg aaatccccag
      361 cgttgtcagg ctttgcagat aagcgatcaa gcatccttct tccccgtggc aagccgttct
      421 gcgcgatcgg cgacattcaa agctgtaatc aactcgtcca gatccccatc catgatctcg
      481 ggaagccgat atgaggtaaa cccgatgcgg tgatcggtca gtcggctttg gggaaagttg
      541 taggttcgaa tcttctcgtt gcgctgtccc gtgcgaacct gcagaccccg gtcgcggcgc
      601 tcctgttccc gctgtgcttg catttccgct tccagaaggc gggaacgaag caccatcttt
      661 gccttggcga gattcttaat ctggcttctt tcatcctggc agctgaccac caaccccgtg
      721 ggaatgtggg tcagtcgaac cgcggagtag gtcgtattga cactctgtcc acccggccct
      781 gaagagcaga accggtcaat gcgcagatcg tcttccttga gattcacgtc cacttcttcg
      841 gcttcgggga gaaccgcaac cgtggctgta gaggtatgaa tgcgccctga ggcttccgtt
      901 gcgggaaccc tctgaacgcg gtgcactccc ccttcaaact tgagaaagct gtacgcccct
      961 tttgcccgaa tggcaaagat cacttctttg aatcccccaa tacccgttgg attgctggac
     1021 agcatttccg ccttccagcc atgacgctct acgtaacgca tgtacattct caagagatca
     1081 gacgcaaaca gcgcggcctc ctcgccaccc gttcctgccc gaatctcgat gatgctattt
     1141 cgatcgtcca cagcctcttt gggcaacaac agatcttcca gatgagcttg tgcctcagtg
     1201 acttgcgctt gcagaatagc cttttcagac tcggcatatt ccctcgtctc ttcgtcctcg
     1261 gacttcagaa gggattccgc ttccgccaaa tctcccaaca actgatccag ttgatccgct
     1321 gcaacaacaa tcggggcaag ttcagaccgc tccttgccaa gactctgaat gcggttgtta
     1381 tcccgaaaga catcgggatt ggacatctcc tggtcaattt ctgccaaacg ttctcggatt
     1441 ttccgaactc tctccagcac gatttcttcc tctattcgcc agtcgaatgc acggtggctg
     1501 accacccccg cctcggggaa tgacgttcaa acgaatatac cacatcgaaa gcaaggtacg
     1561 gatgatattt ctcaacaaat cggcgcatgt caaggaaggc catcgagcca agttcggtcg
     1621 gatttggcct atggagcaat ctttgcttgt cagcgcacct gaaattgtat agattgaatc
     1681 agatgaacga ctattaccga aaatgatttt cgggggtcat gatgatgaac ttttctcgcg
     1741 ttatctgggt cttcgggtgt cttcttctga tccttcccct gtcgcagggt ctggccgatc
     1801 cggctccctc ggttcgatgg tcgggaagca agaacctctc ggaactggaa tacaatgcga
     1861 ttgcaccgac gattctcgcc gatgccaatg gtctgcacat cctgt
//
LOCUS       DAOQUC010000861         1904 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_78422_length_1904_cov_3.354246, whole genome shotgun sequence.
ACCESSION   DAOQUC010000861 DAOQUC010000000
VERSION     DAOQUC010000861.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1904
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78422_length_1904_cov_3.354246"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..443)
                     /locus_tag="PKH07_13435"
     CDS             complement(<1..443)
                     /locus_tag="PKH07_13435"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HNT35986.1"
                     /translation="MINGLYSATSGMFNQIQRSDTIANNIANMETAGFKVIKNTFAQR
                     LSQALYRDSALEPSGPPLVSLQGGGSYIANVYNSFSQGPLRQTGSDFDVALSGKGFLV
                     VDTPSGRMYTRNGNFHPNERREMVTAEGYRVLGTDGTVTLPEGFF"
     gene            890..>1904
                     /gene="lgt"
                     /locus_tag="PKH07_13440"
     CDS             890..>1904
                     /gene="lgt"
                     /locus_tag="PKH07_13440"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HNT35987.1"
                     /translation="MQILGPFVHRIDPIIGTVFGVHLWWYGLSYTLGFLNAFLFVRRK
                     RKQMDLSMRLVYDLSLLLAGCVLLGGRIVEVAFYEWPFYRHHIALIPAYWLGGMATHG
                     LLFGGLIAVWIFSRLQKKSFLSITDMLAIPAAVIMGLGRLGNFVDGQIVGSVTSVPWA
                     FKFPDAEGFRHPVVLYDGLKNLLIVPILVYVGTRRPAQGVRTGIFLFLYAFLRIFIDV
                     FREYPTTLLGLATGQVLNLLMSLIGVGLIVIPLVMRRAELSTDTPSALDVQETLPHAG
                     MIWRRFLFAALLVFALVLPSDWTQDVPTRYGSRHPGLVHSRMYPKINTSPQKVDSNET
                     STLP"
BASE COUNT          398 a          499 c          504 g          503 t
ORIGIN      
        1 tcgaaaaagc cttctggaag cgtgacggtt ccgtctgttc caagaacgcg atatccctcc
       61 gctgtgacca tttctcttcg ctcattgggg tggaagtttc cgttgcgtgt gtacattctg
      121 ccactggggg tatccacgac cagaaacccc ttcccagaga gagcaacgtc aaagtcgctt
      181 cccgtttgtc tcagcggacc ttggctgaag gagttgtaaa cgttggcaat gtaggatccg
      241 ccaccctgca gagagaccag cggaggtcct gaaggttcga gagccgaatc tcggtacagt
      301 gcctggctga gtctctgggc aaaggtattc ttgatgacct tgaagccagc cgtctccatg
      361 ttggcgatat tgttggcaat ggtatccgag cgttgaatct ggttgaacat gcccgatgtc
      421 gcactgtaga gaccattgat catgttgctg cttctccttc agggctttgg tggatttcct
      481 gcaagaagca tatctggcag gttctgctac cttacgcccg ctcagaccct tcgcatccca
      541 tgtgccatgt tcagaaagcc cgcaaaatgg gcgcatcgaa gaagcagagc atctgacgga
      601 attcagacct taacgttagt tctatgaatt gattagctcc aagaaagcgc gatgaaatat
      661 cttgatctca aagtgcaatt ttcctcagag agaagatgct tctctggtaa ctcttgccaa
      721 tgtcaaaagg agtggggcgg aaaatttttc ccataccgag ccgcactcag agagtaggcc
      781 gcctgctctg tttttccagg aacggctggc gggaaaatga aatccttgtc tcatccccag
      841 actctcctgt agaatctggc tgcagaggct tgttacggag gaaaccaaga tgcaaatcct
      901 tggtcctttt gttcaccgga tcgacccgat cattggaacg gtcttcggcg ttcacctttg
      961 gtggtatggg ctcagctaca cgctgggctt cctgaatgcg ttccttttcg tgcggcgcaa
     1021 gcggaagcag atggatcttt cgatgcgatt ggtctatgac ctgagtctcc ttctggcagg
     1081 ttgcgttctc cttggtggtc gcattgtcga agtcgctttc tacgaatggc cattctatcg
     1141 ccaccacatt gcactgattc cggcttactg gcttggcgga atggcaacac atggacttct
     1201 gtttggaggg ttgatcgccg tctggatctt ctcacgactc caaaaaaagt ctttcctgag
     1261 catcacggat atgctggcaa taccggcagc cgtcatcatg ggactcggac ggcttggcaa
     1321 tttcgtagac ggccagatcg ttggaagcgt gacttccgtg ccgtgggcgt tcaagttccc
     1381 cgatgccgag gggtttcgtc atcctgtggt gttgtacgac ggactcaaga accttctcat
     1441 tgttcccatc ctggtttacg tcggaacccg gcgaccagct cagggcgttc gtactggaat
     1501 cttcctgttt ctatacgctt tcctcagaat cttcattgat gtttttcgag agtatccaac
     1561 gacccttctc ggcttggcaa cggggcaagt gctgaacctt ctcatgtcgc ttatcggagt
     1621 cggtttgata gtaataccct tggtgatgcg acgtgcggaa ctttcaaccg acacgccaag
     1681 tgctctcgac gtgcaggaga cactgcctca tgcagggatg atctggcggc ggtttctgtt
     1741 tgccgcgctt ctggtatttg cgttggttct gccaagcgac tggactcagg atgtcccgac
     1801 tcgctatggc agccgccatc caggtctggt gcattcacgg atgtatccca agatcaacac
     1861 ttcaccacag aaggtggaca gcaacgaaac ctctacgctg ccgc
//
LOCUS       DAOQUC010000862         1901 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_78619_length_1901_cov_4.224269, whole genome shotgun sequence.
ACCESSION   DAOQUC010000862 DAOQUC010000000
VERSION     DAOQUC010000862.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1901)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1901)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1901
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78619_length_1901_cov_4.224269"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1029)
                     /locus_tag="PKH07_13445"
     CDS             complement(<1..1029)
                     /locus_tag="PKH07_13445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35988.1"
                     /translation="MRLENSIGLVVVLLISVVLPRAAFTEPISAAGEIGLIPLDSFDH
                     AENWKPIWQFCDPEVIYTANCTPDDDSGVAGKLEYRILKNRGYVGLAAVRPMLLPGKP
                     ERLKIRVYGDGTSNSIVLRIVDAGGETHHVNTGAVTSLGWQEVDVPIDQPEHLSFGGD
                     QNHVLDYPLYFSQILIDRLPEGQEGTIFFADLACVSVVQMHESWSAQLSVRNPYNVLD
                     LDESLSALLEVRSYLAPKREIGVVWKVVTPDGTVVHQEAFPLSVGLHPCRIEIAVPVL
                     EAGFYWLRAETVLDNLRKSVEEPIGLIRKSLWPRPVNQDAFFCINVHDRYTSRELPVL
                     SWAQVGAIR"
     gene            complement(1136..>1901)
                     /locus_tag="PKH07_13450"
     CDS             complement(1136..>1901)
                     /locus_tag="PKH07_13450"
                     /inference="COORDINATES: protein motif:HMM:NF014841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3410 domain-containing protein"
                     /protein_id="HNT35989.1"
                     /translation="GRRVVRKAKSLGMNVLQNDPPRQRVEGGTEFVSFKDALQSDIVT
                     FHVPLTLDGEDTTLHLIDDSLLQLIKPGAIVLNTSRGEVACNSALIKGTDSGLLSAVI
                     LDVWEGEPDVDLSVLSKVTLGTPHIAAYTQQGKVNATQMVYESACRFLGQEPRSDVLE
                     RIKISTDTALQIPDSTTDLESAISFAFRQIYPIGQDDSQLRKIASMDPDKRKHYFDDL
                     RNNYTLRLEASAYRVQLADSQTAWASAFAELGFSVE"
BASE COUNT          472 a          512 c          489 g          428 t
ORIGIN      
        1 tcggatggca cccacttgtg cccatgacaa caccggcagt tcgcggcttg tgtatcggtc
       61 atgaacattg atgcagaaaa aagcatcctg attaaccggc cgtggccaca gtgatttacg
      121 gatcaaacca atgggttcct cgacggattt ccgaaggttg tccaacacag tctctgcccg
      181 caaccagtag aagcctgctt ccagaacggg aaccgcgatc tcgatgcgac agggatgcaa
      241 cccaacgctc aaaggaaatg cttcctgatg aacgacggtt ccatcaggag tgaccacttt
      301 ccagacgacg ccgatttccc ttttcggggc gagataactg cgaacttcaa gcagtgcgct
      361 gagggattca tcaagatcca gaacgttgta tggattccga acggaaagct gtgcagacca
      421 actctcgtgc atttgcacaa cggagacaca ggcaagatcg gcaaagaaga tcgttccctc
      481 ctggccttca gggagccggt caatgagtat ctgcgagaaa taaagcgggt aatccaaaac
      541 gtgattctga tctccaccga aggacagatg ctccggttga tcaatgggaa catccacttc
      601 ctgccaacca agcgatgtga cggctcctgt gttcacgtga tgggtttcgc ccccagcatc
      661 aacgatgcgc agcacgatgc tgttggatgt tccgtcgccg tagactctaa tcttgaggcg
      721 ttccggcttc ccaggcaaaa gcattggtcg aaccgccgca agtccgacgt agcctctatt
      781 cttgagaatc cgatactcca gttttccggc aacaccagag tcatcgtctg gagtgcagtt
      841 ggccgtatag atcacttcag gatcgcagaa ctgccagatc ggtttccagt tttcagcatg
      901 gtcgaaagag tccagtggga tcaagcctat ctcgccagct gcggagattg gctctgtgaa
      961 agcagcacga ggcagaacaa cggagatcag gagaacaacg acaagcccaa tggaattttc
     1021 cagtcgcatg ataccaccat caagacaagc tttattccag catagccatt ttgtgccatc
     1081 tgagtggtga ctgtcaaatt gacacagtcc caggaaaagg ctttctatgg aactgctatt
     1141 ccacagagaa gcccagttcg gcaaaggctg atgcccatgc ggtttggctg tctgccaact
     1201 gcacgcgata tgcagatgct tcaagcctca aagtgtagtt gttgcgcaga tcgtcgaagt
     1261 agtgcttgcg cttgtcggga tccatcgatg cgatttttcg caattgcgag tcgtcttgac
     1321 caatgggata gatctggcga aatgcaaaag atatggccga ttccaaatct gtagtgctat
     1381 ccggaatctg aagtgccgtg tccgtactga tcttaatccg ctccaacacg tcggaacgag
     1441 gctcctgtcc gagaaagcga cacgcagatt cataaaccat ttgcgtggcg ttgaccttgc
     1501 cttgttgcgt ataagcggcg atgtgaggag ttcccaatgt caccttggag agaacgctca
     1561 gatccacgtc cggttctccc tcccacacgt ctaagatcac cgctgagaga agcccggagt
     1621 ccgtcccttt gatgagcgcg ctgttgcagg caacttcccc gcgcgaggtg ttcagcacaa
     1681 tggcaccggg cttgatcagt tgcagcagag aatcatctat cagatgcagt gttgtgtcct
     1741 caccatccaa cgtcagcggg acatgaaacg tcacaatgtc cgactgcaag gcatccttga
     1801 atgacacaaa ttccgttccc ccctcgacgc gttgtcgtgg tggatcgttc tgaagaacgt
     1861 tcatgccgag cgacttggct ttccgaacaa cacgccggcc a
//
LOCUS       DAOQUC010000863         1898 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_78856_length_1898_cov_2.094954, whole genome shotgun sequence.
ACCESSION   DAOQUC010000863 DAOQUC010000000
VERSION     DAOQUC010000863.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1898)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1898)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1898
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78856_length_1898_cov_2.094954"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..512)
                     /locus_tag="PKH07_13455"
     CDS             complement(<1..512)
                     /locus_tag="PKH07_13455"
                     /inference="COORDINATES: protein motif:HMM:NF022112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HNT35990.1"
                     /translation="MNRPSWFPFTVVLLLVLLSETACLAGQVFFVKDLGTFHASQREG
                     LETPQDAVTALLEGPTEAEVSAGVSTAIAKGTKLLDMEVFENGVQINLSEEAGKQLDD
                     QKSELIFLQFFWTMEPFGKEEKVKILVEGKPLSDYIQSVANTRNDKSGNATAKIRTTA
                     FASKAYIVLLT"
     gene            complement(667..>1898)
                     /locus_tag="PKH07_13460"
     CDS             complement(667..>1898)
                     /locus_tag="PKH07_13460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35991.1"
                     /translation="TPSDTPTVTPTDTPTETPSDTPTDTPTETPSDTPTVTPTDTPTE
                     TPSDTPTDTPTETPSDTPTVTPTDTPTETPSDTPTDTPTETPSDTPTVTPTETPTDTP
                     TETPTFTVTDTPTETPTNTPSDTPTETPTETPTDDSTPTNTPTSTPTSTPLAYAVHIL
                     ETTPPIGSVFQPNQLDSLLVTSGYAIQGSTFGVSYAALLYDQSGVLATSSASPVVTKS
                     GTMTVSVSAVMPNSGTVVLKAALVTSAWDILAQDSRVYSIATPTPTPTEWYTMQITST
                     EPDISQPLTTWRLYSIRVRTDYRIITPESGARIHASLWTTSGLLKQNAAVFMAASPNL
                     RTYETTLSSVIPDDGPVILKMRILDENFDIVAEDSRTFAVATPTPTNTPTATNTPVPT
                     PTWPASGLTNDEWDGYQ"
BASE COUNT          389 a          477 c          565 g          467 t
ORIGIN      
        1 gtgagcaaaa cgatatatgc tttcgaagcg aatgcagttg tccgtatctt tgcagtagca
       61 tttcctgatt tgtcattgcg agtattggca acagactgga tatagtctga gagtggcttt
      121 ccctcaacga ggatcttcac cttctcttcc ttgccgaacg gctccatcgt ccagaagaac
      181 tggaggaata tgagttcact cttctggtca tcaagttgct tgccggcctc ttccgaaaga
      241 ttgatctgga caccgttctc aaagacctcc atgtccagaa gtttcgttcc tttggcaatc
      301 gcggtggaaa ctccagcgga aacttcggcc tcggttgggc cttcaagaag cgccgtaacg
      361 gcatcctgtg gtgtttcgag cccctcccgt tgagatgcat ggaaagtccc aaggtctttg
      421 acaaaaaaga cctgccccgc aaggcaggct gtttcagaaa gaagaaccaa aaggaggaca
      481 actgtaaatg gaaaccaaga gggcctgttc atcagattca acaaaacgcc cccgcgttca
      541 agtgtataat acaaaaatcc acgagaacag tatcaccatg ctgcggcccc aattcgaagg
      601 ttgctggcct ctgcgtccaa cagagagttc tcccatcgga gaccggtttc ccaatctcga
      661 acggccctat tggtaaccgt cccactcgtc attcgtcagg ccagacgcag gccaggtcgg
      721 tgttggcaca ggcgtattcg ttgcggttgg tgtgttggtc ggtgtcggag tcgccaccgc
      781 aaaagtcctg gagtcctctg ccacgatgtc aaagttctcg tcaaggatgc gcatcttgag
      841 aatgactggg ccgtcatcgg gtatgaccga agaaagcgtt gtctcgtaag ttctcaggtt
      901 cggcgacgcc gccataaaga cagcagcgtt ctgcttcagc agaccactag tggtccacag
      961 cgacgcgtga atcctggcac ctgactccgg agtgatgatt ctatagtcag tgcgaactcg
     1021 gatgctgtag agtcgccacg tcgtcagagg ttgggaaata tccggttcgg tggacgtgat
     1081 ctgcattgta taccattcgg tcggagtcgg agtcggggtt gcaatgctgt acaccctcga
     1141 gtcctgagcc agaatatccc aagcgcttgt gaccagcgcc gccttgagaa ccacggtgcc
     1201 tgaattgggc atcacggctg agacggacac tgtcatggtg ccagattttg tgactacagg
     1261 agaggcagaa ctggtggcaa gaacgccaga ctgatcatag agcagcgccg catagctgac
     1321 gccgaaggtt gaaccttgaa tcgcgtaacc ggatgtaacc agcaacgagt cgagttgatt
     1381 gggctggaaa accgacccta tgggcggagt ggtctccaaa atgtgaaccg cataggcgag
     1441 cggagtcgaa gtcggcgtac tagttggtgt atttgtcgga gtgctgtcat cagttggcgt
     1501 ttccgtcggc gtctcagttg gagtgtctga aggtgtattg gtcggagtct ccgttggtgt
     1561 gtccgtcact gtaaatgtcg gcgtctccgt cggggtatcc gtcggagttt ccgtcggcgt
     1621 cacggttgga gtatcgctcg gcgtttccgt cggagtgtcc gtcggcgtgt cggatggcgt
     1681 ctctgtcggt gtatccgtcg gtgtcaccgt tggtgtatcg ctcggcgttt ccgtcggagt
     1741 gtccgtcggg gtgtcggatg gcgtctccgt cggggtatcg gtcggtgtca ctgttggagt
     1801 atcgctcggc gtttccgtcg gagtatctgt cggtgtgtca gacggcgtct ccgtcggggt
     1861 atcggtcggt gtcactgttg gagtatcgct cggcgttt
//
LOCUS       DAOQUC010000864         1894 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_79090_length_1894_cov_2.944535, whole genome shotgun sequence.
ACCESSION   DAOQUC010000864 DAOQUC010000000
VERSION     DAOQUC010000864.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1894)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1894)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1894
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79090_length_1894_cov_2.944535"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(83..319)
                     /locus_tag="PKH07_13465"
     CDS             complement(83..319)
                     /locus_tag="PKH07_13465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005996996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HNT35992.1"
                     /translation="MRQMGVGTKTRSCHVSIDVSRCKGCGLCAEVCPKHLIHESKDLS
                     IYGDRVYEYAGEDECTGCLFCALMCPDLAIKIER"
     gene            complement(320..1162)
                     /gene="rfbD"
                     /locus_tag="PKH07_13470"
     CDS             complement(320..1162)
                     /gene="rfbD"
                     /locus_tag="PKH07_13470"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:TIGR01214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HNT35993.1"
                     /translation="MRVLVTGARGMLGSELMPFLAARHEVSGTDIHELDLRDLAATRD
                     RVQCFSPNVIVHCAAMTKVDACEEDPDAAYAVNALATRNVALCSAELGASLVYISTDF
                     VFDGTKQEPYNEYDMVSPVSVYGRSKLAGERYVRQFCPKSFILRTAWLYGKNGWNFVD
                     WVISTMKDKGHLSIVTDQKGSPTWTFDLCRQIETLFMSDRFGLYHASGLGAVTRFDWT
                     QKILTMAGIDKGSLQPITSEQLPQKAKRPANSCLDLMALRLEGLCVMRPWEHSLEDYI
                     KSKE"
     gene            complement(1184..>1894)
                     /locus_tag="PKH07_13475"
     CDS             complement(1184..>1894)
                     /locus_tag="PKH07_13475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNT35994.1"
                     /translation="GGLGTRLHPLTKITNKHLLPVYDKPMIFYPIQTLLNAGIDDILL
                     VTGGNNAGDFLRLLGNGKSMGIRHLEYTYQEGEGGIADALSLAIEFADREKIVVVLGD
                     NIIEKNIRKAVESYQRQDQGARILLKKVPDPERFGVPEIAGNRILAIEEKPKKPKSEY
                     AVIGIYMYDEHVWDIIHTLVPSGRGELEITDVNNAYIQRNQMAYDVLEGWWTDAGTFG
                     SLYKATCLVAEGGANKID"
BASE COUNT          439 a          526 c          459 g          470 t
ORIGIN      
        1 agaagtgcat taggtctgac gcctgccacg gtgacaagca caaccagggc aacgaccacc
       61 agcagagaac atttccatga gatcatcgct caatcttgat tgcgaggtct ggacacatca
      121 gtgcacagaa gagacaaccg gtgcattcat cctctccggc atactcatag acccggtctc
      181 catagatgga gagatccttg gattcatgga ttagatgctt gggacaaact tcggcacaca
      241 ggccgcatcc tttgcagcga ctgacatcaa ttgatacgtg acaagatcgc gttttggttc
      301 caacacccat ttgacgcatt cactccttgc tcttgatgta atcctccagg gaatgctccc
      361 agggacgcat cacgcagaga ccttccagcc tgagtgccat cagatcgagg caggaattcg
      421 ccggtctctt tgccttctgc ggcagttgtt ctgatgtgat gggctgcagt gaacccttgt
      481 ctatcccggc catcgtcaga attttctgag tccaatcgaa tcgagtgact gctccaagac
      541 cggaagcatg atacaaccca aatcggtcgc tcatgaaaag tgtctctatc tggcgacaga
      601 ggtcaaaagt ccatgtggga ctgcctttct gatctgtgac gatgctcaga tgtcccttgt
      661 ccttcattgt ggagatcacc cagtccacga aattccagcc atttttccca tacaaccaag
      721 cggttctcaa gatgaaactc tttgggcaaa actgtctgac atatcgctcg cctgccagtt
      781 tggatcgtcc atacacactg acgggactga ccatatcata ttcattgtag ggttcctgct
      841 tggtaccgtc aaacacgaaa tccgtgctga tgtaaacgag cgatgcgcca agttccgccg
      901 agcacaaggc gacattccgg gttgccaacg cgttgacggc ataggccgca tccggatcct
      961 cctcgcaggc atccaccttg gtcatggctg cgcagtggac gataacattc ggagaaaaac
     1021 actgaacgcg atcacgcgtt gctgccaggt ctcgcagatc caattcgtgg atgtcggttc
     1081 cagatacttc atggcgtgct gcaaggaatg gcatgagttc actgcccagc attcctcttg
     1141 cccctgtgac gagaactctc aaggtagcct ctccacgctg ttgtcagtca atcttgtttg
     1201 ccccgccttc ggcgacgagg caggtcgctt tgtagagtga accgaaggtt cccgcatccg
     1261 tccaccatcc ttccaggacg tcataggcca tctggtttcg ttggatgtag gcgttgttca
     1321 catccgtaat ctccaactca ccgcgtcctg agggaaccaa cgtatgaata atgtcccaaa
     1381 catgctcatc atacatgtag ataccaatga cggcatattc cgacttgggc ttcttcggtt
     1441 tttcctcgat ggcgagtatc ctgttgccag caatctcagg aacgccaaaa cgctcggggt
     1501 cggggacttt cttgagaaga attcgagcgc cctgatcctg tcgctggtaa ctctcgacgg
     1561 ctttcctgat gttcttctcg atgatattgt ctccgagaac aaccacgatc ttctctcggt
     1621 cggcaaactc aatggccaat gagagcgcgt cagcgattcc cccttcgcct tcctgataag
     1681 tatactccag gtgtctgata cccatggact taccattgcc aagaagcctc aggaagtctc
     1741 ctgcgttgtt gccaccggtc accagcagaa tgtcgtctat ccccgcattg agcaaggtct
     1801 ggatagggta gaagatcatc ggtttgtcgt agacgggaag cagatgtttg ttggtaattt
     1861 tcgtaagcgg atgcagcctt gttccaagac cacc
//
LOCUS       DAOQUC010000865         1893 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_79136_length_1893_cov_3.357454, whole genome shotgun sequence.
ACCESSION   DAOQUC010000865 DAOQUC010000000
VERSION     DAOQUC010000865.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1893)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1893)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1893
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79136_length_1893_cov_3.357454"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..575
                     /locus_tag="PKH07_13480"
     CDS             <1..575
                     /locus_tag="PKH07_13480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35995.1"
                     /translation="APYAALALLVGLAVGLPVTLYIQYDLGANMADGWGCNAAPRFTF
                     EEAVRVRERLVTQDALASSDTLTGLQRLSALNPDKKLVGVASLGCMLAIALAFCRIRF
                     AKWPLHPVVLLIGTSYAGCQFCYSFLLGWMLKLLIVKYGGVQYYRRVQILMFGIIAGD
                     MIGGFIPLLVGSLYYFVTQRTPPAFNIFPH"
     gene            complement(605..1108)
                     /locus_tag="PKH07_13485"
     CDS             complement(605..1108)
                     /locus_tag="PKH07_13485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013897747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HNT35996.1"
                     /translation="MQFQEMFRGLPPEMATILTAMIPIGELRASIPIAIKIYQMPVLS
                     AFVFSLIGNMIPVPFILLFIDKVSQFLMKRSPLFERFFNWLFERTRKKFAAQYEKYEL
                     LALAVFVGIPLPVTGAWTGALAAYLFNIPPKKAFWSIGLGVLMAGVIVTLVSLGFFSF
                     LDFLLKV"
     gene            complement(1247..>1893)
                     /locus_tag="PKH07_13490"
     CDS             complement(1247..>1893)
                     /locus_tag="PKH07_13490"
                     /inference="COORDINATES: protein motif:HMM:NF016830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HNT35997.1"
                     /translation="GAVVGVQPVEWFSMNVGVYQGDPDGGRSIEHTLENLSGPMIMVE
                     PAFHYDLCGLAGHIRPGWWWNGTRFDEVDVNDPTPGRFDEAYGCYLVWDQEIWKENPG
                     DEEDSQGVGVFAQYGWSQKDRIECQQYYGAGFQWTGAIPSRDSDITGLGVFHARISDQ
                     AGYEEDSETVVEGFYRLQLLPCVAVKPDIQFIHNPGGSENKDALAVGVRWEVVF"
BASE COUNT          452 a          533 c          479 g          429 t
ORIGIN      
        1 tggctcccta tgccgcactg gcactcctgg tgggactggc agtcggacta ccggttacac
       61 tctacatcca atacgacctg ggggcgaata tggcagacgg ttggggctgt aatgcagccc
      121 ctcgtttcac attcgaagaa gcagtccgcg tccgagaaag acttgtcact caggatgctc
      181 tggcgtcatc ggacactttg accggtctgc agcgactttc cgcattgaat ccggacaaga
      241 aacttgtagg cgttgcatcg ttgggatgta tgctcgccat cgctctggcg ttctgtcgaa
      301 tccgttttgc gaaatggcct cttcatcctg tggtgcttct catcggcacg agctatgccg
      361 gttgccagtt ctgctactcc tttcttctgg gatggatgct gaaactgctc atcgtgaaat
      421 atggcggggt gcaatactat cggcgcgtcc agattctcat gttcggaatc attgcaggag
      481 atatgatcgg cggattcatt cccctgttgg ttgggtcact gtactacttt gtcacacaga
      541 gaaccccacc cgcattcaac atctttcctc attaggtgtc cggtcttgcc aaagatcata
      601 ggggctagac cttcaagaga aaatccagga aggagaagaa gccaaggctg acaagcgtca
      661 caatcacccc cgccataagg actcccagtc cgatgctcca gaatgccttc ttgggaggaa
      721 tattgaaaag ataggctgcc aaggctcccg tccatgcgcc ggtcaccgga aggggaatac
      781 ccacgaacac cgccaacgcg agcagttcgt acttctcgta ctgggcagca aacttctttc
      841 ttgtccgctc aaacagccag ttgaagaatc gctcgaaaag cggcgaacgc ttcatcagga
      901 actgggagac cttgtcaatg aacagcagga tgaagggcac aggaatcatg tttccgataa
      961 gcgaaaacac gaaggcactc aagactggca tttggtagat ctttatcgcg atgggaatcg
     1021 aagcgcgaag ttctccgatg gggatcatgg cggtcagaat ggtggccatc tcagggggaa
     1081 gtccccggaa catctcttga aactgcatct cgaactcctc tggcacatcc ctcgatcaga
     1141 cgtcctgaga caaagaacgt catgggcagt gcaacggcaa tagaacgaac ttgccgtgca
     1201 gtatagtcag agagatcaag aagagcaatc gttattgggg cgacatctaa aagaccactt
     1261 cccaacgcac accaactgca agcgcgtcct tgttttccga accgccagga ttatgtatga
     1321 actggatgtc cggtttgact gctacacagg gaagcagctg cagtctgtag aatccttcga
     1381 cgaccgtttc gctgtcctct tcatatcctg cctgatcgct gattcgggca tggaagacgc
     1441 ccaggccagt gatgtcactg tctcttgagg ggatggcacc cgtccactgg aaacctgctc
     1501 catagtactg ctgacactca atgcgatcct tctgcgacca accgtactgg gcaaagactc
     1561 caactccctg tgagtcttcc tcatcacctg gattttcctt ccagatttcc tgatcccaga
     1621 caagataaca accgtaggct tcatcgaacc gccccggagt tggatcgttg acgtcaactt
     1681 cgtcaaatcg cgtgccgttc caccaccaac ccggtcgaat gtgtcctgca agaccacaga
     1741 gatcatagtg aaatgctggc tcgaccatga tcatgggacc gctcagattc tcgagggtgt
     1801 gctcgatgga gcgtcccccg tcgggatctc cctggtagac acccacgttc atcgagaacc
     1861 attccaccgg ctgcacaccg acaacagccc ccc
//
LOCUS       DAOQUC010000866         1893 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_79149_length_1893_cov_2.857454, whole genome shotgun sequence.
ACCESSION   DAOQUC010000866 DAOQUC010000000
VERSION     DAOQUC010000866.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1893)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1893)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1893
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79149_length_1893_cov_2.857454"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..817
                     /locus_tag="PKH07_13495"
     CDS             <1..817
                     /locus_tag="PKH07_13495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015948595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HNT35998.1"
                     /translation="GTLITPQHAQAFKKAGISYVGVSLDGTQTVNDRFRGVAGAFEKA
                     MVGIENCRAEGIKVGLRFTITKHNHAEVPEVFRILREQKIPRVCFYHLVYSGRGSQMV
                     GEDLDRQQTRSVVDRIINETALLHRNGYPAEVLTVDNHADGVYLYLRLLREDPERAAK
                     VLQLLEYNGGNASGHGIGCVSWDGSVHPDQFWRHLVLGNVRERGFADIWTDRDNELLM
                     KIKNKKSHVTGRCARCRWLGICGGNFRVRAEAVTGDLWAPDPACYLSDEEIGL"
     gene            817..1839
                     /locus_tag="PKH07_13500"
     CDS             817..1839
                     /locus_tag="PKH07_13500"
                     /inference="COORDINATES: protein motif:HMM:NF037131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT35999.1"
                     /translation="MSANPLDQRIFRMIQDGIPIQSRPFDVMAKDLGCSSEDLLNRIK
                     TTVDKGMIRAIGAVFSPQHLGYRTVLCGALVSPACVRTVAEQLNRLHEVTHNYLREHD
                     WNLWFTLVAADTSRIEEILNLVRTNQGVETVVALPARRVFKIAVLFDLDPTRATSSDA
                     QVSVTRARMESPQNLPSPAQIQMVRALQQPFPLVEEPFRAMAAEAKCSEDQLLEQIRR
                     WLSDGTIRRFGARVRHHVVGYAANGMAVWSVPETQVQQFGVCIARFPQVSHCYERQVC
                     PQWRYNLYAMIHAAERQQVKEVHKLISDEIGHWDHEILFSVEEFKKSAPVYFFEKKYG
                     DEEIET"
BASE COUNT          454 a          479 c          535 g          425 t
ORIGIN      
        1 cggaacactg atcaccccac aacacgcgca ggcattcaag aaggccggaa tctcttacgt
       61 tggggtgagt ctggatggaa cgcaaacggt caatgatcgc ttccgtggcg ttgccggtgc
      121 ttttgagaaa gcgatggtcg gcattgagaa ctgtcgcgcc gagggaatca aagttgggct
      181 tcgattcaca atcaccaaac acaaccatgc agaggttccc gaggtcttta gaatactgcg
      241 cgaacagaag atcccgcgcg tctgttttta ccatctcgtc tattccggcc gaggatcaca
      301 gatggttgga gaggatctcg accgccagca aacgcgttct gtggtggatc ggatcatcaa
      361 tgagaccgca cttctgcatc gtaatgggta ccctgcggaa gtgctcactg tggacaacca
      421 tgccgatggc gtttatctct atcttcgctt gttgcgcgaa gatccggagc gcgctgcaaa
      481 ggttctgcaa cttcttgagt acaatggagg aaacgcatca ggccatggca tcggttgtgt
      541 ttcatgggac ggcagcgtcc accccgacca gttctggcgg catcttgttc tcgggaatgt
      601 tcgagaacgg ggttttgccg acatctggac agacagagac aacgaactgc tgatgaagat
      661 caagaacaag aagtcgcacg tgactggccg atgcgcgcgg tgccgctggt tgggcatctg
      721 cggagggaat ttccgcgtcc gtgcggaagc agtgacaggt gatctctggg cacccgatcc
      781 ggcatgttat ctgagcgacg aggagattgg tttgtaatga gtgctaatcc tctcgatcag
      841 agaattttca gaatgataca ggatgggatt ccaatccagt cccgcccttt tgatgtcatg
      901 gcgaaggatc ttggatgcag ttctgaagat ctcctgaatc ggatcaagac aacggttgac
      961 aaggggatga tccgtgcaat tggggctgtg ttcagtcctc agcatctggg atacagaacc
     1021 gttctttgtg gggcattggt gagcccagcc tgcgttcgaa ccgtggcgga acagttgaac
     1081 cgccttcacg aagtcactca caattacttg agagagcatg actggaatct ctggtttact
     1141 ctggtcgctg ctgataccag ccgcatcgag gagattctaa acctcgttcg tactaaccaa
     1201 ggtgttgaaa cggttgttgc cctgcctgcc aggagagtct tcaagatagc cgtgcttttt
     1261 gatctcgatc caacacgtgc aacaagttca gacgctcaag tcagcgtgac cagggcacga
     1321 atggagtctc cgcaaaacct gccaagcccc gctcagattc agatggtcag ggcactgcaa
     1381 cagccctttc cgttagtgga agaaccattt cgcgcaatgg ccgcagaagc caagtgttcg
     1441 gaagatcagt tactcgaaca gattcgaagg tggctctctg acggcacgat tcgacgtttc
     1501 ggtgccaggg tgcgccatca cgtcgtcgga tatgcagcca acggcatggc ggtgtggagc
     1561 gttcccgaga ctcaagtaca gcagttcggg gtgtgcatcg cccgattccc ccaggtgagt
     1621 cactgctacg agcgccaagt gtgtccccaa tggcgctaca acctctacgc tatgattcac
     1681 gccgcggaga gacagcaggt caaagaggtg cacaaactca tatcagatga gattggacac
     1741 tgggatcatg agatcctctt cagtgtcgag gaattcaaaa agtcggcacc agtctatttc
     1801 tttgagaaaa aatacggaga cgaagagatt gagacctgaa gcagttcaag gcacacgatt
     1861 tcagccgcgt ctcatcgcgt gggaactcac ccg
//
LOCUS       DAOQUC010000867         1892 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_79229_length_1892_cov_2.675014, whole genome shotgun sequence.
ACCESSION   DAOQUC010000867 DAOQUC010000000
VERSION     DAOQUC010000867.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1892)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1892)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1892
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79229_length_1892_cov_2.675014"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1520)
                     /locus_tag="PKH07_13505"
     CDS             complement(<1..1520)
                     /locus_tag="PKH07_13505"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNT36000.1"
                     /translation="MMDVNCILEQIPTGVLVCSRLGAIEYANQSLERLIGFPKKDLQR
                     MSLSDLFLHQDILTRCNNISRESRPEPVRVETEMRRADNTTIAVELCASCRSDKPDDD
                     GTLLLFVNDLSRLKELQGDLRHSQSMEALGILAGGIAHDFNNILAAVLGYSELCEASI
                     DENHEAQSYVSQIRQAAGRAKELVGQMLLMSRRTERGCQPVIVSAVVKEVIKLLRSSI
                     ASSASLVSSIDKSEDKVLAEPVQIHQLTMNLFMNAYQALNGANGTIEVELTHVDILPN
                     QVTKHFMKRPGSYVCLRVKDNGSGMDEQTLGNIFKPYFTTKREGTGIGLSNVKGIVRM
                     VNGEIFVQSTLGEGTTFWVYLPVHRESVDQSTPLVESESAGEGEYKEHILFLDDEEPI
                     TNVMRMGLARLGYDVTAFTSSEDALQAFLSSPKRFDVIVSDYQMPSLSGSEVVNRVLQ
                     ERPDIPVVICSGSVEHEQDVLQLKSNCVQFVKKPIEAVELDRVIRKVVLRCRTLAES"
     gene            complement(1528..>1892)
                     /locus_tag="PKH07_13510"
     CDS             complement(1528..>1892)
                     /locus_tag="PKH07_13510"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNT36001.1"
                     /translation="VAAGCPSPRRIRARLPVPTTLPLRGDPLGVFESPRFESRTFPIA
                     SGDRLFLNTDGLVRVQRRDPSTGRTMCIREDALDDLISGISNEPLDGVVGRIWDFVLD
                     FCDDKPDDDMLLVGIEIP"
BASE COUNT          448 a          520 c          453 g          471 t
ORIGIN      
        1 gattcagcga gcgttctgca tcggagaacg actttccgaa taacccgatc cagttccacc
       61 gcttcgatgg gcttcttgac aaactgtaca cagttgctct tcagctgtag cacatcctgc
      121 tcatgctcca ccgatcccga acaaatcacc acagggatgt ctggacgctc ctgcaagacg
      181 cgattgacaa cctcggaacc gctcaatgag ggcatctgat agtcggaaac gatcacgtca
      241 aagcgcttcg gacttgagag aaaggcctgt agggcatctt ctgaactcgt gaatgcagtg
      301 acatcatagc caagtctggc aagacccatc ctcatgacat tggtaatcgg ttcttcatcg
      361 tccaggaaga gtatatgctc cttgtattcg ccttcaccag cggactccga ttccaccagc
      421 ggagtgctct gatcaacaga ttccctatgc accggaaggt aaacccagaa ggtagtgccc
      481 tctcccagag tgctctgtac gaatatctct ccattgacca tgcggacaat tcctttgacg
      541 ttggagagac caataccggt tccctctctc ttcgtggtga agtagggctt gaagatattc
      601 ccaagcgtct gttcgtccat tccactgcca ttgtccttga cgcgaagaca cacgtaagag
      661 cccggtcttt tcatgaaatg cttggttact tggttcggca gaatgtctac atgcgtcaac
      721 tccacttcga ttgtcccgtt ggcgccattc agtgcctgat aggcgttcat gaagaggttc
      781 atggtcagtt gatgaatttg cacaggctca gcgagcacct tgtcttctga tttgtctatg
      841 ctgctaacaa gactcgcgct ggaggcaatc gaactgcgca gaagtttgat gacctcctta
      901 acgacggcac tcacgatgac gggctgacat ccccgttcag tgcgcctgct catgagaagc
      961 atctgaccaa cgagttcctt ggctcgtcct gctgcttgtc ggatctgaga cacataactt
     1021 tgcgcttcat gattctcatc tattgaggcc tcacacagtt ctgagtaacc caaaacagca
     1081 gcaagaatgt tattgaaatc atgggcaata ccaccggcaa gtatgccgag agcctccatg
     1141 ctttggctgt gcctcaagtc tccctgcagt tccttgagtc gggatagatc attgacgaac
     1201 aggagcagcg tcccatcgtc atccggtttg tccgatctgc acgaggcgca tagttccacg
     1261 gcaatggtgg tattgtcagc acgcctcatc tccgtttcaa ctctgacggg ttcaggtctg
     1321 gattcccttg agatattgtt gcaccgtgtg agaatgtctt gatgcaagaa caagtcagaa
     1381 aggctcatcc gctgcagatc cttcttggga aatccaatga gtctttccag agactgattg
     1441 gcgtattcta tcgctcccag tctgctacag accagcacac ctgttggtat ctgctccagg
     1501 atacagttga catccatcat cttttcctta agggatttct atgcccacga gcagcatgtc
     1561 gtcatccggt ttgtcgtcac agaagtcaag aacgaagtcc caaatacgac caacaacacc
     1621 atccaacggt tcattggaaa tgcccgagat cagatcgtcc aaggcatctt ctcgaatgca
     1681 catcgtccta cccgttgacg ggtctcgtcg ctgcacccga actagaccgt cggtgttgag
     1741 aaagagacga tccccagacg cgatcgggaa tgttctgctc tcgaatcttg gtgactcaaa
     1801 cactccaagc gggtcgcccc gtaagggtaa cgttgtagga actggcagac gcgctctgat
     1861 acgtcttgga gaaggacagc ctgctgcaac gg
//
LOCUS       DAOQUC010000868         1890 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_79347_length_1890_cov_3.489373, whole genome shotgun sequence.
ACCESSION   DAOQUC010000868 DAOQUC010000000
VERSION     DAOQUC010000868.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1890
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79347_length_1890_cov_3.489373"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            26..>1890
                     /locus_tag="PKH07_13515"
     CDS             26..>1890
                     /locus_tag="PKH07_13515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015213526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNT36002.1"
                     /translation="MSLSTRRRNFVFRAMQVAALLLVGFIGGYAYFLPSRTMKDVALV
                     SSEVKKQVPLALTVRPDGTAQAVLSKEETGLSISKNHGLLDADGDSSAGNYVLDGRAK
                     VAESKIDLFYDLTEGNTSQGEIFRTNAPATKSMLYTRGGERDLMHAKGAAPSRQVNYG
                     YFAGTADVGNFPVERYAGNENFAQSDLSARGKTEAYDRIVDNPFLTVRDNPLSTFSID
                     VDTASYSNIRRMLQAGQLPPKDAVRIEEMINYFSYDYPQPYGDDPFSVNLVATYAPWA
                     PEHLLVRVGLKGREIENKHRPHSNLVFLIDVSGSMNSPNKLPLVKDSLRLLVRQLGGT
                     DRVAMVVYASTTGLVLPSTTGADQRAILDAIERLEAGGSTDGGSGIQLAYDTATQNFI
                     QGGINRVILCTDGDFNVGITNEGDLTRLIEDRAKSGVFLTVLGFGMGNYKDSTLEKLA
                     DKGNGNYAYIDTQNEARKVLVEQMSGTLVTIAKDVKIQVEFNPVEVAGYRLIGYENRI
                     LAKEDFNDDTKDAGEIGAGHTVTALYEVIPAGHDVPSTKVEDLKYQTAASFTKYSETG
                     EMLTVKLRYKKPDGDTSKLLEYPLPAEGDSFEQASEDFRFAAAVAGRRAHGVRAVR"
BASE COUNT          455 a          495 c          535 g          405 t
ORIGIN      
        1 gaaagcttca gagaagacac gtattgtgag cctttcgaca aggcggagga attttgtctt
       61 ccgagccatg caggtcgccg cacttctgct tgtcggtttc attggcgggt atgcttactt
      121 cttgccgagt cggactatga aggatgtggc attggtgtca tccgaggtga agaagcaggt
      181 tcctctggcg ctcactgttc gtcctgacgg aaccgcacag gcagttctct ccaaggaaga
      241 aaccgggctt tcgatttcga aaaaccacgg tcttcttgat gcggatggag acagttctgc
      301 tggcaactac gtcctcgatg gcagagcaaa ggtcgcagag tccaagattg acctctttta
      361 cgacctaacc gagggaaaca catcgcaggg agagatcttt cgtaccaatg cccccgctac
      421 caagagtatg ctgtacactc gaggggggga gagagatcta atgcatgcca agggtgcagc
      481 gccatccaga caggtgaact acggctactt tgccggtact gccgatgtcg ggaactttcc
      541 tgtggagcgg tacgcgggca acgagaattt cgctcaatcg gatctgtcgg caagaggaaa
      601 aacggaagct tatgaccgaa tcgtggacaa cccatttctc acggtgagag acaatccgct
      661 ctcaaccttt tcgatagacg tggacaccgc atcctactcg aacatccgac gaatgcttca
      721 ggcagggcag ttgcccccca aggatgccgt gcggatcgag gagatgatca actacttctc
      781 gtatgactac ccgcagccct acggggacga tcctttctcc gtgaaccttg tggcaaccta
      841 tgcgccttgg gctccggaac atctgctcgt tcgggttgga ctgaaggggc gcgaaatcga
      901 gaacaagcat cgtccacaca gcaatctcgt gttcctgatc gatgtgtcag gttcgatgaa
      961 cagtccgaac aaactgcctc tggtcaaaga ctcactgcgg ctgctagtca ggcaactcgg
     1021 aggcacggat cgcgtcgcca tggtggtcta cgcatctacc acgggactgg ttcttccatc
     1081 cacgacagga gccgatcaac gtgccattct cgacgccatc gagcgcttgg aagcgggggg
     1141 ttccaccgac ggtgggtcag ggatccaact ggcctatgac accgccactc agaacttcat
     1201 ccagggtggc atcaaccgtg tgattctctg caccgatggg gatttcaatg ttggcatcac
     1261 caatgaaggc gacctgacac ggctgatcga ggacagagcc aagagtggtg tcttcctgac
     1321 cgttctcggt ttcggcatgg gtaactacaa ggactcgacg ctggagaaac tggcggacaa
     1381 aggaaacgga aactacgcct acattgacac acagaatgaa gccagaaaag tgttggtgga
     1441 acagatgtcg ggaactctgg tgaccattgc caaggacgtg aagattcagg ttgagttcaa
     1501 ccctgttgaa gttgcgggct accgcctcat tggctacgag aaccggattc ttgccaagga
     1561 agacttcaac gatgacacca aggatgccgg agagattgga gcgggtcata ctgtcacagc
     1621 actctatgag gtcattcctg ccggtcatga cgttccttcc accaaggttg aggatttgaa
     1681 ataccaaact gctgcgtctt tcacgaagta ctccgaaacc ggtgagatgc tgacggtaaa
     1741 acttcgctac aaaaaaccag acggtgacac gagcaaactg ctcgagtacc cactcccggc
     1801 ggaaggggat tcctttgaac aggcttcgga agactttcgg ttcgctgctg cggttgcagg
     1861 gcggcgcgcc catggggttc gggcagttcg
//
LOCUS       DAOQUC010000869         1890 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_79359_length_1890_cov_2.989646, whole genome shotgun sequence.
ACCESSION   DAOQUC010000869 DAOQUC010000000
VERSION     DAOQUC010000869.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1890
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79359_length_1890_cov_2.989646"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="PKH07_13520"
     CDS             <1..298
                     /locus_tag="PKH07_13520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36003.1"
                     /translation="KVFRNYQAGWLTMASLRNVIALEDGGTRRLVPPYPLAPREVSRS
                     MPGALSFPCALLLDVTQAFVPMIFCSESPDTDKNVCDTLSGDAIHVVSTAGNAG"
     gene            complement(305..1150)
                     /locus_tag="PKH07_13525"
     CDS             complement(305..1150)
                     /locus_tag="PKH07_13525"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HNT36004.1"
                     /translation="MHSEQTDRHLETDRIYNMDCIKGMQEMPEECVDLIITDPPFAID
                     FRAQRDNYNRTHSRVLEGYNEIAAEDYLDFTRAWMRQAFRVLSPSGSTFVFSGWNNLK
                     DLLIAADEIGFVTVNHIIWKYQFGVVCKKRFVTSHYHCLFLCKDDGRRRFFNSSRFSG
                     DDRTENGRSLRYQDLEDVWTIKREYWHGDTKTPTKLPAELIVKILSYTSEQGDLVLDP
                     FLGSGQVAVVSKMNKRRFIGFEIVEDYYRFALERLESGQYRLPSDDKQAKPRGMDHQT
                     DLFSL"
     gene            complement(1343..>1890)
                     /locus_tag="PKH07_13530"
     CDS             complement(1343..>1890)
                     /locus_tag="PKH07_13530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014462969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HNT36005.1"
                     /translation="RDEMRILESASESEQIANEGGTSDDVFVVPAFAGLGAPHWDMYA
                     RGAIFGLTRDTGRAQIIKATLESLAYQTRDVLDAMVKDTGLELTSLQVDGGASANNYL
                     MQFQADILGVEVDRPQIIESTALGAALLAGLASGTWDLSSIRSIRTVDRTFTPTMSAK
                     RREHLYLRWQEAVRRTMNWAT"
BASE COUNT          434 a          509 c          477 g          470 t
ORIGIN      
        1 gaaagtcttt cggaattatc aggctggatg gttgacaatg gcatcgctga ggaacgtgat
       61 cgcgttggaa gatggtggaa ctcgcagact cgttccaccc tacccactgg cacccagaga
      121 agtcagccgg agcatgccag gtgccctctc tttcccgtgt gcgctgcttc tcgatgtgac
      181 acaggcattc gtgccgatga tcttctgttc tgaatcaccg gacacagaca agaatgtctg
      241 tgacacactg tccggagatg ccatacacgt tgtctctacg gcggggaacg cgggataaat
      301 cccgctacaa agagaacaaa tcagtctggt gatccatccc acggggcttg gcttgtttgt
      361 catcgctggg gaggcggtac tgtccacttt ccagacgctc cagagcaaag cggtagtagt
      421 cttcgacaat ctcaaaaccg atgaatcgcc tcttgttcat cttactcacc acggcaacct
      481 gtccggaacc aagaaaagga tcgagaacaa gatctccctg ctcacttgtg tagctgagga
      541 tcttcacgat cagttctgcc ggtaacttcg tcggtgtctt ggtgtctccg tgccaatact
      601 cacgtttgat cgtccacacg tcttcgagat cctggtatct gagactgcga ccgttttccg
      661 ttcggtcgtc gccgctgaag cgggaactgt tgaagaatct ccgtcgtcca tcgtccttgc
      721 agagaaaaag gcaatggtaa tgagatgtca cgaagcgttt cttacagact actccgaact
      781 ggtacttcca gatgatgtga ttgaccgtca caaatccaat ttcgtccgcg gcgatcagca
      841 agtctttgag attgttccaa ccggagaaga cgaatgtact tccggaaggt gacagaactc
      901 gaaacgcttg gcgcatccac gcgcgcgtga aatcaagata gtcttccgcg gcaatctcgt
      961 tgtacccctc aagcactcta gagtgtgttc ggttgtagtt gtcccgctgt gccctgaaat
     1021 caatggcaaa gggggggtca gtgatgatca ggtcaacgca ttcttcgggc atttcctgca
     1081 tccctttgat gcagtccata ttgtagatcc gatcagtttc caagtgtctg tctgtctgtt
     1141 ctgaatgcat ttgcagtgtc cttaccgata tgcggtttga cggtacagta acatatttat
     1201 gattgtcatg gatgtagatt tcgtccgaac tggacatctc ccctcgctac caaagggagt
     1261 gtacgcctga ctgtttgccg aagccaagcc ggtcatacat ctggaacggc cgagagtatg
     1321 caaagagctg aaccggagag tgtcatgttg cccaattcat tgttcgcctg actgcctctt
     1381 gccagcgaag gtagaggtgc tcacgccgtt tggcgctcat cgtaggagta aaggttctat
     1441 ccaccgtccg gatgctcctg atactgctca agtcccaggt accacttgcc agacctgcca
     1501 gtagtgcagc ccccaaggcg gtggactcga taatctgagg gcgatccact tcaacgccga
     1561 gaatatccgc ctgaaactgc atcagataat tgttggctga cgcgccaccg tccacctgga
     1621 gacttgtgag ttccagtcca gtatctttca ccatggcatc cagaacatcc cgagtctgat
     1681 aggccaaaga ttccagggtt gctttgatga tctgagcgcg gccggtgtct cgtgtcagtc
     1741 cgaaaatcgc cccacgtgcg tacatatccc aatgaggtgc tcccaaacca gcaaatgcgg
     1801 gcacgacaaa gacatcatct gaagtgcctc cctcattcgc gatttgctcc gactcggaag
     1861 ccgattccaa gattctcatt tcatctctga
//
LOCUS       DAOQUC010000870         1886 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_79611_length_1886_cov_3.333151, whole genome shotgun sequence.
ACCESSION   DAOQUC010000870 DAOQUC010000000
VERSION     DAOQUC010000870.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1886
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79611_length_1886_cov_3.333151"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1886
                     /locus_tag="PKH07_13535"
     CDS             <1..>1886
                     /locus_tag="PKH07_13535"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HNT36006.1"
                     /translation="LTESAWEKATLLPDFNNAIGSASPHLQTEVRLIRDDRSLCVAFI
                     CFDPLVEFIQTTSTRHDSPVWQEDHVAVFLEPAGSSDSYSAFGVNAFGIRAEARYPDL
                     GTEWDAMWLAKAGKRQGYWIAEIQIPFSALAFDQAPGTVWRFNAVRYVARTDTFYSWA
                     PVEKSVHEPRNFGRLLEMKVDLSRHGFGGSLQELFPCVLGANRIDFVIHNQTRQKKRM
                     RARVETTDPEGKINYTFSDFKLAAGEKRVVEMSYNLFKPGQHFLDLIVEDTSTRENVL
                     AFLDIPVTMPVPLELVLDRSVYTSEEYARLQILFRAHQKYYKGMVCAVHVLSETDGKI
                     LLEMNATTNLISGEPVALYLPSLLKDQPPQAGLKIKAQVRDSEGVLIASTDASLRRSP
                     QERIPIQIDKVTGLVILDDKPFLPLGVYWADMREMSDIASAGFNICGVTCTPADFIER
                     QTVFDEAERNNLRTLCGFSDLHGPSGLHKESLVMMAFYFKPRASLVAWNVAHEPAETG
                     VSVDQVKEIASVVREVDPTRPLQITENWPERFADDADLSDILCIEPDTEDRPLPYAVR
                     DQVAAARPILGSGKTLWVAFPSPEEATNNLKTYRNASYLALLQGARGVFFYRQDPSQT
                     TSWL"
BASE COUNT          421 a          544 c          503 g          418 t
ORIGIN      
        1 aactgaccga atccgcatgg gagaaagcaa cactgcttcc ggacttcaac aacgccattg
       61 gctcagcgtc ccctcatctg cagacggagg tccgcctaat acgcgatgac cgttctcttt
      121 gtgtggcgtt catctgcttc gacccattgg tggaattcat ccaaaccaca tccactcgtc
      181 acgactctcc cgtctggcag gaagaccatg tggcggtatt cctggaacct gcgggttcat
      241 cggactcata ctccgcattt ggggtcaacg cattcggcat ccgcgcagaa gcacgctacc
      301 cggatctggg taccgagtgg gacgccatgt ggctggcaaa agcggggaag cgacagggat
      361 actggatcgc cgagattcag attcccttct ccgccctggc cttcgatcag gcaccaggca
      421 ccgtatggcg tttcaatgcc gtccgctatg tcgctcgaac cgatactttc tacagttggg
      481 cacccgtgga aaagagtgtc catgaaccca ggaacttcgg ccgactgctg gagatgaaag
      541 tggatctgtc ccggcacggg tttggtggct cgcttcagga gttgtttccc tgtgttctgg
      601 gcgcaaacag aattgacttc gtgattcaca atcagacaag acagaagaaa cggatgcggg
      661 cgcgtgtgga gaccaccgat cccgaaggca agatcaacta caccttctcc gatttcaaac
      721 tggccgcagg agagaagcgt gttgtggaaa tgtcgtacaa cctcttcaag ccggggcaac
      781 atttcctgga cttgatcgtg gaagacacgt ctacccgtga gaatgtgctg gcgtttctcg
      841 acatccccgt gacgatgccg gtacccttgg aattggtact ggatcgctcc gtctatacat
      901 ccgaagaata tgcccgactt cagatcctct tccgtgccca tcagaagtac tacaaaggga
      961 tggtctgcgc cgttcacgtt ctcagtgaga cggacggaaa gattctgctc gaaatgaacg
     1021 cgaccaccaa tctgatttca ggagagccgg ttgcgctcta tcttccatca ctcttgaagg
     1081 atcaaccgcc ccaagctggc ctcaagatca aggcacaggt gcgagattcc gagggagttc
     1141 tgattgcctc gacagacgca agtctacgcc gatcccctca ggagcgaatc ccgatccaga
     1201 tcgataaggt cacaggtctc gtgatactcg atgacaaacc gtttctgccc ttgggtgtct
     1261 actgggcaga catgcgcgaa atgtcagaca tcgcctcagc gggcttcaac atctgcggcg
     1321 tcacctgcac accagcggac ttcatcgagc gtcaaaccgt ctttgatgaa gccgaacgca
     1381 acaatctcag aaccctctgt ggattttccg atcttcacgg gccgtctggt cttcacaagg
     1441 aatccctcgt catgatggcc ttctacttca agcctcgtgc cagccttgtt gcctggaatg
     1501 ttgcccatga acctgcagaa actggcgtct ctgtggatca ggtcaaagag atcgcttcgg
     1561 ttgtgcgtga agtggatccg actcggcctc tccagatcac agagaactgg cctgaacgtt
     1621 tcgcggatga cgccgatctg tctgacattc tgtgcatcga accggacaca gaggacaggc
     1681 ctcttcccta tgctgtgcgc gaccaggtcg ccgcggcacg accgatcctg ggaagtggca
     1741 agacgctctg ggtggcgttc ccatcgcctg aggaggccac aaacaatctg aagacgtacc
     1801 gaaacgcctc ttatctggcg ctgcttcagg gtgcgagagg cgttttcttc tatcggcagg
     1861 atccaagcca gactacctca tggctg
//
LOCUS       DAOQUC010000871         1882 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_79889_length_1882_cov_3.024631, whole genome shotgun sequence.
ACCESSION   DAOQUC010000871 DAOQUC010000000
VERSION     DAOQUC010000871.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1882
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79889_length_1882_cov_3.024631"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            34..>1882
                     /gene="uvrA"
                     /locus_tag="PKH07_13540"
     CDS             34..>1882
                     /gene="uvrA"
                     /locus_tag="PKH07_13540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HNT36007.1"
                     /translation="MDLISGLSPAISIEQKAAIRNPRSTVGTVTEIYDYLRVLYARIG
                     LPHCPKCGRAVGAQTLDQIVDRILLLESGTRFLVLAPIVRGRKGEYREILDEARREGF
                     LRVRINGTVRSLEDEIQLDKRMKHDLEIVVDRLVLREEIRSRLTDSVSTALHLGDGVV
                     VISLVDKNEDLFYSERHACAACGLSLEDLTPQHFSFNSPHGMCPVCDGLGQKLRFDPD
                     LIIPEKTLSISKGAIAPLRRVLDARGVSRQGKVIQKALEIVGEDFGFDLNVPWEQLSP
                     QQQNVVLYGIGRDNYCQARWSKRTAFPGIIPQLEKWLEGPELSGARSHFTEGYMSMAV
                     CPECMGGRLRSESAAVTLNERTIVDVVRMSIRECLKFFSNLTLTPTEKTIAEEVLREI
                     SGRLQFLENVGLHYLTLDRSAPSLSGGEAQRIRLASQIGSGLVGVLYILDEPSIGLHQ
                     KDNRQLLQTLLRLRDLGNTVLVVEHDAEIIHNADWIVDFGPGAGELGGEIVASGTLDT
                     ILQSKDSLTGKFLSGKRSIPIPAKRRTPSSKWLTVRGARENNLKSIDVSFPLGLITCV
                     TGVSGSGKSSLVNEILYNWLARTLNRAQTSPGTCDAVEGAEWLDKVINID"
BASE COUNT          423 a          507 c          531 g          421 t
ORIGIN      
        1 cgacagttcc tgggacaact ggagaaacca aaggtggatc tgatctccgg actgtcgccg
       61 gctatctcca tcgaacagaa ggcagcgatc cgcaatcccc gttccacggt cggaacggtg
      121 acagagatct atgactacct tcgggtactc tacgcccgga tcggacttcc ccattgcccc
      181 aagtgtggca gagccgttgg tgcacagacg ctcgatcaga ttgtggatcg gattctgctt
      241 ctggaatcgg gaacccgatt cctggtcttg gcgcccatcg tccgcggccg aaaaggggag
      301 tatcgggaaa tcctcgatga agcgcgacgc gagggatttc tccgggtccg aatcaacgga
      361 accgttcgct ctcttgaaga tgaaatccaa ctggacaaac gcatgaaaca cgatcttgag
      421 atcgtcgtcg atcgccttgt tcttcgcgag gagattcgtt cccgtctcac cgattcggtg
      481 agcacggcac tgcacctggg tgatggggtt gtggtgatca gcctggtgga caaaaatgag
      541 gatcttttct acagcgaacg acatgcctgc gctgcctgtg gtctgtcctt ggaggatttg
      601 acacctcagc acttttcttt caacagtcct catggaatgt gtcctgtttg tgatggattg
      661 gggcagaaac tgagatttga tccggatttg atcattccgg agaagactct ttccatctcc
      721 aagggtgcca ttgcaccgct gcgaagagtt ctggatgcca ggggagtctc ccgacagggc
      781 aaggtcattc agaaggcact tgagatcgtt ggagaggatt ttggattcga tctgaacgtg
      841 ccttgggagc aactgagtcc ccaacagcag aatgtcgtgc tgtacggaat cggacgcgac
      901 aactactgcc aggcgcgatg gagcaaacgc actgcctttc ccggcatcat cccgcaactt
      961 gaaaaatggc tggaaggtcc cgaactgagc ggagcccgtt cccatttcac cgaaggctac
     1021 atgagcatgg ccgtctgccc cgaatgcatg ggaggccgcc tgcggtcgga gagtgctgcc
     1081 gtaacactga atgaacgaac catcgtagat gttgttcgaa tgtccatcag ggaatgcctc
     1141 aagtttttct cgaacctcac tctgaccccg acggagaaga ccattgcgga agaagtcctg
     1201 cgcgagattt ccggccgcct tcagtttctc gaaaatgtcg gcctgcatta tctgacgctg
     1261 gatcgctccg ctccgagtct ctcgggtgga gaggctcaac gcattcgcct ggcaagccag
     1321 atcggatcgg gtctggtggg cgtgctctac attcttgatg aaccttccat cggactgcat
     1381 cagaaagata accgccaact gcttcagact ctgctccggc ttcgcgatct gggaaacacg
     1441 gtcttggtgg tcgaacacga cgccgaaatc atccacaacg cagactggat cgttgatttc
     1501 ggtcctggag cgggagaact gggcggcgag attgtcgcct ctggaacact ggacacaata
     1561 ttgcagtcca aagactcgct cacagggaag tttctatcgg gcaagcgatc gatcccgatt
     1621 cccgccaaga ggagaactcc cagttcgaag tggctgactg ttcgcggtgc gcgtgagaat
     1681 aatctgaaaa gcattgatgt ctctttccca ctggggctga tcacctgtgt cacaggagtc
     1741 tccggttcag gcaaatcgtc tctggtcaat gaaattctat acaactggct cgcgcggacg
     1801 ttgaatcgag cccagaccag ccccggaacc tgtgatgccg tcgagggcgc agagtggctg
     1861 gacaaggtca tcaacattga cc
//
LOCUS       DAOQUC010000872         1882 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_79891_length_1882_cov_3.006568, whole genome shotgun sequence.
ACCESSION   DAOQUC010000872 DAOQUC010000000
VERSION     DAOQUC010000872.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1882
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79891_length_1882_cov_3.006568"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..832)
                     /locus_tag="PKH07_13545"
     CDS             complement(<1..832)
                     /locus_tag="PKH07_13545"
                     /inference="COORDINATES: protein motif:HMM:NF024755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HNT36008.1"
                     /translation="MVGFGGRALSAQDDAKYLNSPETELFKKGQILYGLYQAREAIKK
                     EKQVIVSEGYMDVIALHQFGFENAVAVLGTAMTNDHVRRLKRYTDQILLVFDADRAGQ
                     SAAVRSTETLSQNDLRGRVLCLPRGEDPDSFLRQQGQDAWTKLVTNEALVPFDFMMKQ
                     ARERNPGPSIESKSAVGRELLQFIAGLPRAMDRSLHLGLLAAQLGIPQESLARDLNAL
                     RGKREGVVKPLGQGMKSHASTAADAAKRGLVVLVLNSDSASRIGGERIRALSSLIDDT
                     E"
     gene            complement(886..1440)
                     /locus_tag="PKH07_13550"
                     /pseudo
     CDS             complement(886..1440)
                     /locus_tag="PKH07_13550"
                     /inference="COORDINATES: protein motif:HMM:NF013930.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CHC2 zinc finger domain-containing protein"
     gene            complement(1482..>1882)
                     /locus_tag="PKH07_13555"
     CDS             complement(1482..>1882)
                     /locus_tag="PKH07_13555"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010349630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNT36009.1"
                     /translation="VVAQGQLAWPIEPGKFKITSRFGERHSSPHQGVDFSAPRGTTIR
                     AADDGVVIVASENLGGYGKTVVLHHPNGLRTVYGHADTLCVRLGQSVKRGQKIAEVGS
                     TGRSTGFHLHFEVRVSADKPVDPMGYLTSK"
BASE COUNT          438 a          580 c          457 g          407 t
ORIGIN      
        1 gctctgtatc gtctatcaga gacgacaaag cccgaattct ctccccacca atccgggaag
       61 cagagtcgga attcagaacg agtacaacca atccacgctt ggccgcatcg gctgccgttg
      121 aggcgtggct cttcatgccc tgtccgaggg gcttgaccac accctcacgc ttgcctcgca
      181 gcgcgttcag atcccgtgcc agcgattcct ggggtattcc cagttgtgcg gccagcagac
      241 ccaaatgcaa actgcggtcc atggcacgtg gcaacccagc aatgaactgg agcagctcac
      301 gcccgaccgc tgacttggac tctatagaag ggcccggatt tctctcccga gcctgcttca
      361 tcatgaaatc gaacggtaca agtgcctcat ttgtaacgag ctttgtccaa gcatcttgtc
      421 cctgttgacg cagaaaactg tccggatcct ctcctcgggg cagacagagc actctccccc
      481 gaagatcgtt ctgggaaagc gtttcagtac tgcgtaccgc cgcactctgc cccgcccggt
      541 cggcgtcaaa gaccagcaat atctggtcgg tatatctttt caagcgacga acatggtcat
      601 ttgtcatggc ggttcccagc accgcaaccg cgttctcaaa cccgaactgg tgcagggcaa
      661 tcacgtccat gtacccctct gagacaatga cctgcttctc tttctttatc gcttcccgtg
      721 cctgatagag accatagagt atttgaccct tcttgaaaag ctccgtctct ggactgttga
      781 gatacttggc atcatcctgc gccgacaggg ctcggccacc aaaaccgacc acatgaccca
      841 aagcatccgt aatcggaaat atgatccgat cccgaaaccg tcataatggt tgccactctt
      901 ggcacctagc agtatcaaac cagccttctc ggcatccgga aaggacacct gccaccgatg
      961 aagctgcttg cacaagtcat cccaggccgc gggagcatag cctaaacgaa accgaacctg
     1021 gatttcctcg gaaacccccc gctgtctgag atactcccga gcctgttttc ccagaggaga
     1081 tttgagcagg cagtgatgat agtagcctgc gacctgctcc aacagtcctc tgcgctcatg
     1141 atcctcttct ggtgacgaac cttcaacata gcgaagcgtc acaccggcgc gctgagcgag
     1201 gtacttgacc gcctcagcgg gagccagact ttccatctct tgaacgaaag ttatcgcatt
     1261 cccacctttg cgacaaccga aacagtagaa aagtcctttt gaggggctga ctgtgaagga
     1321 gggggttttc tcgtcatgga agggacaaag ccctttgaag cggtcccccg tacccttgag
     1381 agtggggaca tgatcggaga tgagagagac cagatcggtc ttctccagta tctcatccac
     1441 aagtcccggt gcaaaacgtg ccacgaaaaa ccaccgctcc tctactttga cgtcagatat
     1501 cccatcggat caaccggttt gtcggcggaa acccgcactt cgaaatgcag atgaaagcct
     1561 gttgatctac cggtggagcc cacttcagcg atcttctgac ctcgcttgac agactgtcca
     1621 agtcgaacgc aaagagtgtc cgcatgacca taaacggttc taaggccgtt tggatgatgc
     1681 agaacgaccg tttttccata gccgcccaga ttctctgagg caacgatgac caccccatca
     1741 tccgccgcgc ggatcgtggt tcctctgggt gccgaaaaat ccacgccctg atggggactg
     1801 gaatgccttt ccccaaaacg ggacgttatc ttaaacttgc ccggctctat cggccatgcc
     1861 aactgccctt gcgccacaac gg
//
LOCUS       DAOQUC010000873         1882 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_79912_length_1882_cov_2.151615, whole genome shotgun sequence.
ACCESSION   DAOQUC010000873 DAOQUC010000000
VERSION     DAOQUC010000873.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1882
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79912_length_1882_cov_2.151615"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..981
                     /locus_tag="PKH07_13560"
     CDS             <1..981
                     /locus_tag="PKH07_13560"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HNT36010.1"
                     /translation="HYAKKGAEVTLICATLGEAGMVDPRFLQNGQTVAGLREQELRCS
                     AEALGLKDAFLLGYRDSGMQGSPDNTHPQALAAQKLEDVVTKIAQYMRALQPDIVLTF
                     DPFGGYGHPLFTTEEMLERLYRIGLYFVAIGIQTGSEHVNRDIYNRRIPNEKIMNLAW
                     SAKKVGVSELVYDVLTNNPYETEEDCRATLELLLAMPKPSKLTVSKLVMFPQNKILEL
                     TVPKVNLPETVFDCWNLLYLMSASPLFTMDQVRQWSNISDLHNNPSVLEKLVVQLCHA
                     VEEKQQSEAALDAERSTTGQLRSELEICRHWKDRPLFERAIRKIMRAFSS"
     gene            complement(1337..>1882)
                     /locus_tag="PKH07_13565"
     CDS             complement(1337..>1882)
                     /locus_tag="PKH07_13565"
                     /inference="COORDINATES: protein motif:HMM:NF024764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNT36011.1"
                     /translation="LDRAVECFDWLVEFNPINFLFWSKRGQAYTVLGDLADPQAYNKA
                     AADLQRALTLAPNASELHGYLGQVYVRQQLWDRAESATKTAIQMDSTNTYHRRTLGNI
                     FAAQQRFLEAEEAFRRILQLDPKDADAINNLGVALAQQGKTEEARAAFRDALRLQPSL
                     QDARINLNRLDGEPSTPGGRF"
BASE COUNT          438 a          513 c          507 g          424 t
ORIGIN      
        1 cactacgcga aaaaaggcgc cgaggtcacc ctgatctgcg cgacactggg ggaagccggc
       61 atggttgacc cgcgtttcct gcagaatggg cagaccgtcg ccggcttgcg cgaacaggaa
      121 ctgcgctgct ccgccgaagc gctcgggctg aaagatgcgt ttttactggg ctaccgcgat
      181 tcgggcatgc agggctcccc ggacaacacc cacccgcagg cgctggcagc ccaaaagctg
      241 gaggacgtcg tcacaaaaat cgcgcagtac atgcgcgcgc ttcagccgga tatcgtgctc
      301 acctttgacc cctttggcgg ttacgggcat cctctcttca ctacggaaga gatgttggag
      361 agactctacc ggatcggtct gtactttgtt gccataggaa ttcagactgg cagcgagcat
      421 gtcaatagag acatctacaa ccgtcgaatc ccgaatgaga agatcatgaa tctcgcttgg
      481 tcggcaaaga aggttggggt gagtgaactg gtctacgatg tgctgaccaa caacccctac
      541 gagacagagg aagactgtcg cgccacactc gaattgcttc ttgccatgcc caagccctcc
      601 aaactgacgg tcagcaaact ggtcatgttt ccccagaaca agattcttga gttgacagtc
      661 cccaaagtga accttcccga gacggttttt gactgctgga atctcctgta tctaatgtcg
      721 gcatcgccgt tgtttacgat ggatcaagtc aggcagtggt caaacatatc ggacttgcat
      781 aacaatccct cagttctcga aaaactggtt gtgcaactgt gccatgccgt tgaagagaaa
      841 caacagtcag aagcggcctt agacgccgaa cgttcgacaa ctgggcaact tcgatcagaa
      901 ctcgaaatct gcagacattg gaaagatcga cctctcttcg aacgtgccat ccggaaaatc
      961 atgagagcat tcagtagcta gtgtagcgtc aaccgtggtc tgtcgcatcg agatggccga
     1021 aggaggaaac tctcaaattg tcattctgag tggagtccgc ggaacgaaga atctctccgt
     1081 atgctcttga cctcgccttg cttgaagaga gattcttcac ttcgctccca aagcctcgct
     1141 tcgttcagaa tgacatattg tcccagggca aagcggtcaa aaggcgctcc caacctctcg
     1201 accgtcattt cgggcttgac atgacactag tctgaagcaa tttgtgccgt agtaacctct
     1261 ctactccgac caggagatta tgttttggca gatgtctgat ctgatggaca gaatcgccgg
     1321 cgggcgtcca cttgggtcag aacctcccgc cgggcgtgga tggctctccg tcaagtcggt
     1381 tgaggttgat tctggcgtcc tgtaaggacg gttgcagacg caaggcgtcc cgaaaagcgg
     1441 cacgtgcttc ctcggtcttg ccctgttgtg ccaaagccac cccgaggttg ttgatagcat
     1501 cggcatcctt tggatccagt tgcagaatgc gcctgaaggc ttcttccgct tccaggaagc
     1561 gctgttgcgc cgcaaagatg tttccgagtg ttcggcggtg ataggtgttt gtggagtcca
     1621 tctgaatggc ggtcttcgtt gctgattcag ccctatccca caactgctga cgaacgtata
     1681 cctgcccaag atagccatgc agttcggaag catttggagc cagagtcagc gctctctgaa
     1741 ggtcggcagc ggccttgttg taagcctgag gatctgcaag gtcgcccaga accgtgtatg
     1801 cctgtcccct ctttgaccag aagaggaaat tgatggggtt aaactcaaca agccagtcaa
     1861 agcactccac cgcccggtca ag
//
LOCUS       DAOQUC010000874         1881 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_79960_length_1881_cov_2.579409, whole genome shotgun sequence.
ACCESSION   DAOQUC010000874 DAOQUC010000000
VERSION     DAOQUC010000874.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1881)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1881)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1881
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79960_length_1881_cov_2.579409"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..556
                     /locus_tag="PKH07_13570"
     CDS             <1..556
                     /locus_tag="PKH07_13570"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNT36012.1"
                     /translation="ARLCETKGLGVLIEAFILLKQSGRHPSLRLRVAGTLAPGDSRFV
                     KKVQARLTSEGLAKDVQFLFNLSMESKLDFLKSLTVFSVPALCSESFGLYVIESLAAG
                     VPVVEPNHSAFPELLKETEGGLLCETNSPASLAEKVDFLLSHPDQARSLGQTGQKYVR
                     ERFNTGLMAERFLKLLQEIAADEV"
     gene            608..1684
                     /locus_tag="PKH07_13575"
     CDS             608..1684
                     /locus_tag="PKH07_13575"
                     /inference="COORDINATES: protein motif:HMM:NF015084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate protein ligase C-terminal
                     domain-containing protein"
                     /protein_id="HNT36013.1"
                     /translation="MTLSFRHIEDTNPLSSADGLAGDEALVSGLADGNSPPTLRLYRY
                     HPSAIVGRYQNLTDAINLDTCRRYGVSWNRRHTGGGTVLMGPEQVAIALALPDGRDSE
                     RRTIKQHFEFFAAILADALGAFGIEAAFVGKNDLQVDGRKIAGLAISQDTDGAVFYHG
                     SLLLDFDVGLMVQVLNLPTEHLEDRGQSCFSRRMTTVREHVPGASFPDLTAAIARSLE
                     SLTHVQLCRSSFLDHEHEAIARLKRERYENEEWICSSRVMRRWRGMVERKTAAGTIRI
                     YVDGSGPALDAVLITGDYFSRNLELATLESNLKGTPARSDAIASVISKCGQGTIYRLT
                     AEELAELIVMARQSNTTARLTSQA"
     gene            complement(1681..>1881)
                     /locus_tag="PKH07_13580"
     CDS             complement(1681..>1881)
                     /locus_tag="PKH07_13580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36014.1"
                     /translation="QFPQARLTLGCAKTWGKLQRCFEEKALELGYDAIAYPSEGIIQL
                     ARQMGYTVKLTESCCAFLGMIG"
BASE COUNT          418 a          490 c          519 g          454 t
ORIGIN      
        1 tgccagactc tgtgagacaa aagggcttgg tgtcctaatc gaagccttca tccttctgaa
       61 gcagagcggt cgccatccat cactcagact tcgggttgct ggtacgttgg cacctgggga
      121 tagccgtttt gtaaagaagg ttcaggcacg gctcacttca gagggtttgg cgaaggatgt
      181 gcagttcttg ttcaatcttt cgatggagtc gaaactggat ttcctcaaat ccttgacagt
      241 cttctccgtt cccgcgctgt gcagtgagtc tttcggcctc tacgtcatcg aatccttggc
      301 cgcaggggtt cccgttgtgg aacccaacca cagtgcgttc cctgaacttc tgaaagagac
      361 cgaaggcggg cttctctgtg agacaaacag cccagcatca cttgctgaga aagttgattt
      421 tctgttgtcg catcccgatc aggcgcgttc tctgggtcaa accggccaaa agtatgttcg
      481 ggaacggttc aacacaggtc tgatggcaga gcggttcctg aaattgcttc aggagatagc
      541 agctgatgaa gtctgacaca acacgcgtac agaacgcatc gctgcaatct ggagttcaca
      601 ggcactcatg actctttcgt ttcgccatat cgaagataca aatcctcttt cttccgctga
      661 cggtctcgca ggtgacgagg ctttggtatc cggactggca gatgggaact caccgcccac
      721 gctgaggctg taccggtacc acccctctgc gatcgtcggt cggtatcaga atctgacaga
      781 tgcaataaat ctcgatacgt gtcgccgata tggcgtgtca tggaatcgga gacacacggg
      841 tgggggaacc gtcctcatgg ggccggaaca agttgccatt gctttggctc tgcccgatgg
      901 cagggactcc gaaaggcgaa cgatcaagca gcattttgag ttcttcgcgg cgattcttgc
      961 cgatgccctt ggggcttttg gaatagaggc agccttcgtc ggaaagaatg atcttcaagt
     1021 tgatggtcgc aagatagcag gactggcgat ttctcaggac accgatggag cggtcttcta
     1081 ccacggcagt ctgcttctgg atttcgacgt cggtctcatg gtgcaggtgc tgaatcttcc
     1141 cacagaacat cttgaggatc ggggtcaaag ttgtttctcc agaagaatga ccacggtgcg
     1201 tgaacatgtg ccaggtgctt ccttccctga tctgactgcc gcgattgccc gctctcttga
     1261 gagtctcacc cacgtccaac tctgcagatc gagttttctt gatcatgagc atgaggccat
     1321 tgcccgactc aagagagaac gttacgagaa tgaggagtgg atctgttcgt ccagagtcat
     1381 gcgccgatgg cgtggcatgg tggaacgcaa gacggctgca ggtaccattc ggatctacgt
     1441 tgatggaagt ggacctgctc tggatgctgt gttgatcacg ggtgattact tcagtcggaa
     1501 cctggaactt gctacgctgg agtcaaatct caaaggaacc cccgcccgat cagacgccat
     1561 cgcctccgtc atttccaaat gtggacaggg aacgatttac cgtcttacgg ctgaggaact
     1621 cgctgagctg atcgtcatgg cacgccagtc caacactacg gcacggctca ccagtcaggc
     1681 ctaaccgatc atgccgagga aagcacagca ggattccgtc agtttgaccg tatagcccat
     1741 ttgacgggcg agttggatga tgccttcgga aggatacgca atcgcatcgt aaccaagttc
     1801 gagtgctttc tcttcaaaac atcgctgtag tttgccccac gttttggcgc aacccagagt
     1861 gaggcgcgcc tgaggaaatt g
//
LOCUS       DAOQUC010000875         1877 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_80227_length_1877_cov_2.826015, whole genome shotgun sequence.
ACCESSION   DAOQUC010000875 DAOQUC010000000
VERSION     DAOQUC010000875.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1877)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1877)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1877
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80227_length_1877_cov_2.826015"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1197)
                     /locus_tag="PKH07_13585"
     CDS             complement(<1..1197)
                     /locus_tag="PKH07_13585"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNT36015.1"
                     /translation="MRNRTRLPKRVILFFCCLLAFWPLGECCSQQGTPPSTGNPSGVA
                     AGQSQPAWLSPFSLSWFPLHSGYEVGALGLSGLDYNLLSVVTPTYTPTATPIGQLEWT
                     TYNVANSGLNHNEVTCILVDATRRMWFGTNNGLCLYDGVSWQTFTKENSGIWGNYISA
                     ITQDYRGRVIVGMLLGWAQNNGIAIFDNGVWTSHWLADDSRTGKSTYDPTCLTTDQQG
                     RIWIGTTDEGLWLMDGSRWRRYTMTNSGLPWNRILSLAFDAQGKLWIGTKGRGYSVFD
                     PVTETFVTSLIPFITPTPTQTPTRTPTITPSPTMTPTNPDDTPMNITPTNTATATPTL
                     EHPTPSSPMQNWVTGFEFLPDGRAWLLGNKDGFALKDGDDWSLFNQSVLKVTPEQEIT
                     GLRDVQY"
     gene            complement(1397..>1877)
                     /locus_tag="PKH07_13590"
     CDS             complement(1397..>1877)
                     /locus_tag="PKH07_13590"
                     /inference="COORDINATES: protein motif:HMM:NF024246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNT36016.1"
                     /translation="RGLYFGIKTCLAAHVTTGGDVICPYGCLGLGDCFNVCAFDAIRM
                     EKGSPVVDANKCTACGKCVAACPRKLITLHDYDPQKGLVAVVCSSRDRGPEVKKVCEV
                     GCIACKVCEKQGPENVFKVEDNLSAMNYELYTDASACQLAIEKCPTHCIAVINIRTS"
BASE COUNT          449 a          496 c          495 g          437 t
ORIGIN      
        1 gtactgaacg tcacgcaaac ccgtgatttc ctgctccggt gttaccttca gaacgctctg
       61 attgaacaaa gaccagtcgt caccatcttt caatgcgaag ccatctttgt tacccaggag
      121 ccaagcacga ccatcaggca ggaactcaaa tccggtcacc caattctgca tcggagaact
      181 gggggtcggg tgttcaagtg tcggcgtggc ggtggccgta ttcgtcggag tgatattcat
      241 cggcgtgtcg tctggattgg tcggtgtcat ggtcggcgat ggggtgatcg tcggtgtgcg
      301 agtcggcgtt tgtgtcggcg ttggcgtgat gaagggaatc agcgaagtga caaaggtttc
      361 cgtgacagga tcgaagaccg agtatcccct tcccttggtt ccgatccaca gtttcccctg
      421 agcgtcaaac gcaagcgaga ggattcggtt ccaaggaagc ccggagttgg tcattgtgta
      481 tcggcgccat ctgctgccgt ccatgagcca gagaccctca tcggttgttc cgatccagat
      541 tcgtccctgt tgatcagtgg tgagacacgt cggatcatag gtagacttgc ccgtacggga
      601 atcgtccgcg agccaatgag atgtccacac gccgttgtca aagatggcaa taccgttgtt
      661 ctgcgcccat ccgagcaaca taccgacgat aactctgccc cgatagtcct gagtgatggc
      721 ggagatgtag ttaccccata taccgctgtt ctctttggta aatgtttgcc aggaaacacc
      781 atcgtagaga catagtccat tgttggtgcc aaaccacatt cttctggtgg catccaccag
      841 aatacaggta acttcgttgt gattcagacc tgagttggca acattgtacg tcgtccactc
      901 cagttggccg attggagtgg cggtaggagt ataggtcggt gtgacgacgg aaagaaggtt
      961 gtagtccaga ccggacaacc cgagtgctcc aacttcatag cccgaatgca gcggaaacca
     1021 actgagcgaa aagggggaaa gccaggcagg ttggctctga cctgctgcga caccagaagg
     1081 gttgcctgtg cttggtggcg ttccctgttg cgaacagcat tctcccaacg gccagaaggc
     1141 caggagacag cagaagaaga gaatcactct cttgggaagt ctagttctgt tcctcatcaa
     1201 cacatcccga atccaatatg acgccctatg gctgacgtgt aagtcatatc agaattcaga
     1261 atcgctgtca cctttactaa ctccttccac gtcgcacagg cgactcagaa gactgctccg
     1321 gttggtttgc gctgcgaact tggcaaggac attgaacccg ttgagaaaaa gaggctttct
     1381 gaaagccaca accatatcaa ctggttcgga tgttgatcac tgcaatgcag tgcgtcggac
     1441 acttctcaat ggcgagctga caggcagaag catcggtgta aagctcgtag ttcatagcgg
     1501 aaaggttgtc ttccaccttg aatacattct ctggcccctg tttttcacag actttgcagg
     1561 caatacagcc aacttcacaa accttcttca cctcaggtcc cctgtcccgc gaactgcaaa
     1621 ccacagcaac aagacctttc tgtggatcgt agtcatggag ggtgatcagt tttctggggc
     1681 aggccgcgac acatttcccg caggcagtgc acttgttggc atctacaaca ggagaaccct
     1741 tttccatgcg aatggcatcg aaggcacaga cattgaaaca gtcccccaag cccagacatc
     1801 cgtagggaca tattacatcg ccacctgtcg tgacatgtgc tgccaggcag gtcttgattc
     1861 caaaatacaa acctcgc
//
LOCUS       DAOQUC010000876         1876 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_80280_length_1876_cov_3.264690, whole genome shotgun sequence.
ACCESSION   DAOQUC010000876 DAOQUC010000000
VERSION     DAOQUC010000876.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1876)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1876)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1876
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80280_length_1876_cov_3.264690"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /locus_tag="PKH07_13595"
     CDS             complement(<1..478)
                     /locus_tag="PKH07_13595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36017.1"
                     /translation="MEEKISGDMFRGVSPALLQALQSRMDPEEVLNAINFFPESAQRF
                     PGKFRCFCPIHREKVIRTMTIDTQKKRFSCRYARCKGSKGGDLLTLYALGTRCSPTEA
                     VDYWVKRLKLDPSQYPASTLPAEQSSEDVYTLEDLIRSEKPPEEALTTEPTPSQEPV"
     gene            complement(588..1670)
                     /gene="mutY"
                     /locus_tag="PKH07_13600"
     CDS             complement(588..1670)
                     /gene="mutY"
                     /locus_tag="PKH07_13600"
                     /inference="COORDINATES: protein motif:HMM:TIGR01084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HNT36018.1"
                     /translation="MHSKWLFFTLSRMSRFEKLAGIIENWFRHNARVLPWRTDYDPYK
                     VWVAEIMLQQTQVKKVIPFFLRFVERFPTLAELAGASEREVLLHWAGLGYYSRARNLL
                     KGVQTVRKLYRGRVPSEIPQLMELPGIGRYTAGAIASIAYNQPEPLVDGNVIRLLSRL
                     FAVEEHPEKPTGKAKLWSLATSLVNSATPRLLNQGLMELGALICTPQKPQCPACPLRR
                     LCKARRLKRTEQYPRRSESSETLKIHETFLVISRRGRYLLVQRGRGVSYQGLWEFPRE
                     ESLSTESLQKGKEPLGVLNYRIMNRQVEAHFFSAEFEGAFALLCPYVDSRWLTLRQSQ
                     RYPVPAPIRKLISTLLKEVHTAVDGP"
BASE COUNT          455 a          493 c          481 g          447 t
ORIGIN      
        1 aaacaggttc ttgcgatgga gtcggctcgg ttgtcaaggc ctcttccgga ggcttctcag
       61 acctaatcag gtcctccagc gtataaacgt cctctgaact ttgctctgca ggcagagtgg
      121 atgcaggata ctgcgatggg tctagtttca gtcttttgac ccagtagtca actgcttcgg
      181 ttggagaaca gcgtgttccc aatgcgtaga gtgtcagtag atcgccaccc tttgagccct
      241 tacagcgcgc gtatcggcag ctgaatcgct tcttctgggt gtctatggtc atggtgcgaa
      301 tgactttttc gcgatgaatc gggcagaaac agcggaactt tcccggaaaa cgctgagcac
      361 tctctgggaa gaaattgatg gcgttcagaa cctcttccgg atccatcctg ctttgcagag
      421 cttgcagaag tgccggagag acccctcgaa acatgtcacc ggatatcttt tcttccactt
      481 ccttgaggcc accacatctg aatttctacc ttgctattga tacacctaaa tcagaaaatc
      541 ggcaagcaca gtgtcacaga atcaggatct gccacactcc gcaattctta agggccgtca
      601 accgctgtat ggacttcctt gagaagagtc gaaatgagtt ttcggatcgg tgcgggcaca
      661 ggatagcgct gggattgcct gagggtgagc cagcgactat caacgtaggg acacagaagc
      721 gcgaaggctc cttcaaactc agcactgaag aaatgagcct ctacctgacg gttcatgatc
      781 cgataattga gcacacccaa aggctccttc cctttttgca gagattcggt tgagaggctc
      841 tcttctctgg gaaattccca cagcccctga tacgaaacac ctcgtcctct ttgaaccagg
      901 aggtacctgc cacgtcgtga gatgacaaga aaagtctcgt gaatcttcaa cgtttccgaa
      961 gattcggatc gtctgggata ctgttctgtc ctcttgagcc tccttgcttt gcagagcctg
     1021 cgtaaaggac aagccggaca ctgaggtttt tggggcgtac agatgagtgc accaagttcc
     1081 atcagcccct gattcagcaa gcggggggtg gcagaattga ccaaagatgt ggcaaggctc
     1141 cagagtttgg ccttgcccgt tggcttttcg gggtgctcct caacagcaaa caaccgagac
     1201 agcagtctga tgacatttcc gtcaaccaga ggttcgggct gattgtacgc gatgcttgca
     1261 atggctccag ctgtgtacct tccgatcccg ggaagttcca taagttgtgg gatttccgaa
     1321 ggaacgcgcc cccgatacag tttcctgaca gtctggactc ccttgagaag atttcgggcg
     1381 cgagagtaat accccaagcc agcccagtgc agaaggactt cgcgctcaga ggcaccagcc
     1441 agttctgcta gcgtgggaaa acgctcaaca aaccgaagga agaaggggat cactttcttg
     1501 acctgggtct gctgcagcat gatctcagca acccatacct tgtagggatc atagtctgta
     1561 cgccaaggca ggacacgggc attgtgacga aaccagttct cgattatgcc tgccagcttc
     1621 tcaaatctgc tcatacgcga aagagtaaag aacagccact tcgaatgcaa ctacagtcca
     1681 gattccagca cttgagaagg gctgcgctgg atatacttcc cgtggccaca gaaggtcgag
     1741 acgcagtcag ccttccgatc cgtaacactc tatatgggtg agacttcaca caaagatgac
     1801 aagctggctc tgaacgattt tcccagaatg tgctcctcgg attctgtcac taaggtttca
     1861 gaccaagttc tttgct
//
LOCUS       DAOQUC010000877         1876 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_80289_length_1876_cov_3.045030, whole genome shotgun sequence.
ACCESSION   DAOQUC010000877 DAOQUC010000000
VERSION     DAOQUC010000877.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1876)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1876)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1876
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80289_length_1876_cov_3.045030"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(253..786)
                     /locus_tag="PKH07_13605"
     CDS             complement(253..786)
                     /locus_tag="PKH07_13605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HNT36019.1"
                     /translation="MRDQQKIEARQRACAMLEKANISITEQERREMEVADFGLGILDK
                     IGLEIVVYINCDRYCAKELILFPWQVCPEHRHPMRQGVPGKQETFRCRWGEVYLYAEG
                     EKTANPKGRSPAREEHFTVWHEIVLKPGEQFTLPSNTLHWFQAGPEGAIVSEFSSPSD
                     DESDIFTDPQIQRVEKP"
     gene            1043..1132
                     /locus_tag="PKH07_13610"
     tRNA            1043..1132
                     /locus_tag="PKH07_13610"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1077..1079,aa:Ser,seq:gct)
     gene            complement(1275..>1876)
                     /locus_tag="PKH07_13615"
     CDS             complement(1275..>1876)
                     /locus_tag="PKH07_13615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HNT36020.1"
                     /translation="FILGGKYYCCGIGDLQVVFDNPTRRKVVEVFRQFVPELVFGAPL
                     SDYMLDHEMAGQLIRDAAFTASLPNYSTGQENPAPHLPKVPHLYYMQPLGNSNVLGEP
                     VLPEFCIDITTVMETKKEMLAQHASQREWLRVQHKMDQYIINMQQESEAIGKLIGVPY
                     AEGFRLHKASGHPRSNVLQELLPEYIHPLSKNLLNKMSV"
BASE COUNT          424 a          481 c          494 g          477 t
ORIGIN      
        1 gtggcatggc cgcttgtcgg ccatgaaaca ggcagacatg cccgattagc gggcatggca
       61 cccggcaagg gagcgtgggc ggctcaagat gtcattctga acgcagtgaa gaatctctct
      121 tgtttcctcc gctacagtcg agcctgaaga gagatgcttc gcctagccta agcatgacaa
      181 gaccgtagga gggggcgaga cccatcagat agaaatcccc aatcctacca tcctgcatct
      241 gttgtggcgc actcagggtt tctcgactct ctggatttgc gggtcggtga aaatatcgct
      301 ttcatcgtcg cttggagagg agaactccga aacgatcgct ccttcggggc ctgcttggaa
      361 ccaatggagc gtgtttgaag gaagcgtaaa ctgttcgccc ggcttcaaaa cgatttcatg
      421 ccaaacggtg aaatgctctt ctcgggctgg cgatctccct tttggattcg cggtcttttc
      481 gccttcggcg tagagataga cttcgcccca ccggcagcgg aatgtctcct gttttccggg
      541 cacaccctgt cgcatcggat ggcgatgttc cgggcatacc tgccatggaa acaggatcag
      601 ttccttggcg cagtatcggt cgcagttgat gtagacgaca atctccaaac cgatcttgtc
      661 caggattccc aagccgaaat ccgccacttc catctcacgg cgttcctgtt ccgtgatgga
      721 gatattcgct ttctcaagca tggcgcaggc tctttgtcgt gcttcaatct tctgctggtc
      781 tctcatttgt gtgctttctc ctggtatcgg ttaaacgttc tatctcaaga gccctcaaaa
      841 ccatgctctt gagagtgtgg agtcgaaatc cgatacagac agtccttact ctgtgtcctt
      901 acaacggatc actttgaggc acaataacag caaaaaacaa agcgtttttc aagctgaact
      961 cgcgttcagg aggaaaagtg aaaatgctgt tgacagcatg aagaaggctt tctattctca
     1021 tcatgacatt tgagcagatt ctggagaggt ggccgagtgg tcgaaggcgg caccctgcta
     1081 aggtgttgag ggggtaactc ctccctgggt tcaaatccca gcctctccgc cagtcttctt
     1141 tttcttccat gcatagtccg aatacttgat aaagtgaaag cgttctgctg gacaacaagg
     1201 acaggcctgt cttgtccgga aaatgtactc tgcggacagt gtgtaccaag cctgacgttt
     1261 tctcatccgg caatctagac tgacatctta ttgagaaggt ttttcgacaa agggtggatg
     1321 tattccggca gcaattcctg caagacgttg ctcctcggat ggccacttgc cttgtgcaga
     1381 cgaaatccct cggcgtaggg gactccaatc agtttcccta ttgcctcgga ttcctgctgc
     1441 atgttgatga tgtattgatc catcttgtgc tgcactcgaa gccattcacg ctggctggca
     1501 tgttgggcaa gcatctcctt cttagtttcc atcaccgttg tgatgtcaat gcagaactcg
     1561 gggagcacag gctctccaag aacattgctg ttgcccagtg gttgcatata gtaaagatgc
     1621 ggaaccttcg gaagatgagg agcaggattc tcctggccag tgctgtagtt gggaagcgat
     1681 gcggtgaagg cggcatctcg gatgagttgt cccgccatct catggtcaag catgtagtcc
     1741 gagagaggag cgccgaaaac tagttctggt acgaactgtc tgaaaacctc aacgaccttg
     1801 cgtcgggtgg gattgtcaaa aaccacctgc agatcgccga taccgcagca gtaatacttt
     1861 cccccgagga tgaagg
//
LOCUS       DAOQUC010000878         1875 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_80349_length_1875_cov_3.467033, whole genome shotgun sequence.
ACCESSION   DAOQUC010000878 DAOQUC010000000
VERSION     DAOQUC010000878.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1875)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1875)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1875
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80349_length_1875_cov_3.467033"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..357)
                     /locus_tag="PKH07_13620"
     CDS             complement(<1..357)
                     /locus_tag="PKH07_13620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36021.1"
                     /translation="MYSIKAPAVYVTERANADPRCRARMQRLMHGVQTNCVRCVSDEE
                     LNEIVGDNALVPTARTGAMGGGSDTDERILLLDAFPWHSVDAEKAYAAQYQGLRAVGV
                     LGPWTIRDCVHLRRDFD"
     gene            541..>1875
                     /locus_tag="PKH07_13625"
     CDS             541..>1875
                     /locus_tag="PKH07_13625"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNT36022.1"
                     /translation="MFFSTRCFPAFVAFILCCFPRISYADSARPPEKEEYFSANGEFS
                     LTVDPGDWTSESVTRLILKSEKEGIILDVTESYFEKFRYPLHACVSNDAEYLVFGGVS
                     CHNMGSGREGLRIYRRTGRLIRFIPMARLPYGFGSASTDHWYDQERCRINDSRGLFLL
                     YTPGKMRATPFRLTDGRAYPKSRTLSCSRRSNHFKETAESNLSQTKDRYSDLPEVRVW
                     GIPLLRLVFRVLGSRPERRDDLGLSLALLLYRQSPRFILKQSTEGSPEWRAVCYRALA
                     MNATTYVEEDGTPLFPEELDEKHIRGIAESLASDETWVAEGATQLTCFVESPQFILIL
                     EKRLRDESLDPSLRSRSVQGYAKNTGTDAVPLLVELLENSEDDRVREQCARELGNLRD
                     PRAFSALLRVADQESYSQNPQEREDLRVQSLIALRKIGDSRALETLENILRRK"
BASE COUNT          460 a          482 c          499 g          434 t
ORIGIN      
        1 gtcgaaatcc cgacgcagat gcacgcagtc cctgatcgtc cagggtccca gaacaccgac
       61 tgctctgagg ccctgatact gcgcggcata ggccttctct gcatccacgg aatgccaggg
      121 gaacgcgtca agaaggagta tgcgctcatc cgtatcggat cctcctccca tagcaccggt
      181 tcgagcggtt gggacaagtg cattgtcacc gacaatctca ttgagttcct catcagaaac
      241 gcatcgaacg cagttggtct gaacaccgtg catcaggcgc tgcattcgcg cacggcatcg
      301 cggatcagcg tttgcacgct cggtgacata gacagcaggt gctttgattg agtacatttt
      361 cggtcagcac tcctcagctc tttgagtatc atccgaactg gattctaccg gcaagggaat
      421 ttctgctcaa gtcgttgcgt cgcggagaaa agcggctaat tgttggacgc tcattagcca
      481 actgggatat cattactcac gaaagaacaa gcacagcaag ctggaatcag gaggagacgg
      541 atgttctttt cgactcgatg ttttcctgcc ttcgttgcat tcatcctctg ctgcttccct
      601 cggatctcat atgcagattc cgcaagaccg cctgaaaagg aggaatactt ctctgccaat
      661 ggggagttct ccctgaccgt ggatccggga gactggactt ctgagtccgt cactcgactg
      721 attcttaaga gcgagaaaga aggcatcatt ctggacgtca ctgagtctta ctttgagaag
      781 tttcgctatc ccttacatgc ctgcgtttcg aacgatgctg agtatctggt cttcggagga
      841 gtttcttgtc acaatatggg gagtggaaga gaaggactgc gaatctatcg aagaacaggc
      901 aggctgatcc gattcatccc catggctcgg ttgccctacg gtttcggcag cgcatcaaca
      961 gatcattggt atgaccagga aaggtgccgc atcaatgaca gtcgaggtct gtttctactt
     1021 tacacaccag gcaaaatgag agcaactccc tttcggctca ccgatggacg tgcctatcca
     1081 aagtcacgca ctctcagttg ttcgagaaga tccaatcact tcaaggaaac tgcagaaagc
     1141 aatctgtctc aaacaaaaga cagatactcc gatcttcctg aggtcagggt ctggggaata
     1201 ccgttgctgc gacttgtatt ccgtgttctg ggatcaagac ctgaacgtag ggacgatctt
     1261 gggctgagtc tggcgctgct tctctatcgc cagagtcctc ggtttatact caagcaatcc
     1321 acggagggca gccccgaatg gagggcggtc tgctatcgcg cattggcgat gaatgcaacg
     1381 acctacgttg aagaagacgg cactcctttg tttcctgaag agttggatga aaagcacata
     1441 cgagggatcg cggaatccct tgcctcggat gagacctggg tcgcagaagg cgcaacccaa
     1501 cttacgtgct tcgttgagtc tccgcaattt attctgattc ttgagaagag actgagagat
     1561 gaatcgctcg atccatcgct cagatcgaga agcgtacaag gctatgcgaa gaacactggc
     1621 acggacgccg ttccgctgct tgtggaacta ctcgagaact cggaagacga ccgagtcagg
     1681 gagcagtgcg ccagagaact tgggaatctc cgtgatccga gagccttttc tgcactgttg
     1741 cgagtcgcag accaggagag ctattcccag aacccacaag agcgtgaaga tctgagagtg
     1801 caatctttga tcgctttgcg caagataggg gattcaagag cccttgagac cctcgagaac
     1861 atactgagaa ggaag
//
LOCUS       DAOQUC010000879         1873 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_80509_length_1873_cov_3.159516, whole genome shotgun sequence.
ACCESSION   DAOQUC010000879 DAOQUC010000000
VERSION     DAOQUC010000879.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1873)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1873)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1873
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80509_length_1873_cov_3.159516"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..462)
                     /locus_tag="PKH07_13630"
     CDS             complement(<1..462)
                     /locus_tag="PKH07_13630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018707416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HNT36023.1"
                     /translation="MAHHLFTSESVTEGHPDKVCDQISDAILDSIIKDDHDARVACET
                     LVTTGLVMIAGEITTSTYVDIPEIARNTVKRIGYTDASFGFDYETCAVLTSIHAQSPD
                     IAMGVDESEEHQQGAGDQGMMFGYACDETEELMPLPITLAHRLTMRLAKVRK"
     gene            complement(507..1526)
                     /locus_tag="PKH07_13635"
     CDS             complement(507..1526)
                     /locus_tag="PKH07_13635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012501945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNT36024.1"
                     /translation="MTGEGTKQTSEPSRIRMAVIPVAGEGTRLRPLTHTTPKVLINVA
                     GKPILGHILDKLESLGIEEVVLIVGHLGRLIVEYVRDKYPFKTHVVEQEKRLGLGHAV
                     SLVRDKVGSQPFLIILGDTIFEADFTEVLCGDMSYIGVKEVVDPRRFGTVALRDGIIS
                     RMVEKAENPPSNLAMVGIYALCNAPLLFECLDHIISHDIRTKGEYQLTDAMQMMIERG
                     EVMKAFPIEGWFDCGKAETVLETNRALLESSSNEVSLPGCIVIPPVYVARSAGVQNSI
                     IGPYVTIADNSVVNDSVIRNSIINESAVVRNMLLKESLVGNNAVVIGRPMRLNVGDSS
                     EISFE"
BASE COUNT          452 a          528 c          440 g          453 t
ORIGIN      
        1 tttgcgtacc ttcgccagtc tcatcgtgag tctgtgcgcc aaggtgatgg gcaacggcat
       61 gagttcttca gtttcatcac acgcatagcc aaacatcatg ccttggtctc ccgcgccctg
      121 ctggtgctcc tcgctctcgt ccacacccat ggcaatatcg ggtgactgtg catgaattga
      181 agtcagaacg gcgcaggtct cgtagtcaaa cccaaaagag gcatcagtat atccaatgcg
      241 tttgaccgta tttcgagcga tttccggaat atccacatag gtactcgtcg tgatctcgcc
      301 cgcaatcatc accaaacccg ttgtcaccag agtttcacag gcgactcggg catcgtgatc
      361 gtccttgatg atggaatcca ggatcgcgtc ggatatctga tcgcagacct tgtccggatg
      421 gccttcagtg accgactcag aagtgaacag atgatgtgcc attgactctc ctctttcttg
      481 acaaaacttt ttcgagcgcc tgacagtcac tcgaaactga tctcggagga atccccgacg
      541 ttcaaacgca tgggacgtcc gatgacgacc gcattgttgc ctaccaggga ttctttcaaa
      601 agcatgtttc tgactacagc gctttcattg ataatcgaat tccgaatcac ggaatcattg
      661 acaacagaat tgtctgcaat ggtgacatag ggaccgatga tcgagttctg aacccccgca
      721 ctccgcgcta catagacagg cggaatcacg atgcaaccgg gcagcgagac ttcattggaa
      781 ctgctctcca acagcgccct gttggtctcc aagaccgttt cggctttgcc acagtcgaac
      841 caaccttcga tgggaaaagc cttcatgacc tcaccccgct cgatcatcat ctgcatcgcg
      901 tcagtcaact ggtattcccc cttggttcga atgtcgtgag agattatatg atcaagacac
      961 tcaaacagaa gaggcgcatt gcacaatgcg tatatgccca ccatcgccag attcgaaggg
     1021 ggattctctg ccttctcaac catccgactg ataatgccat ctcgaagggc gactgtgcca
     1081 aatcgccttg gatcgactac ttccttgacc ccgatataag acatgtcccc acacagaact
     1141 tctgtaaaat cagcctcaaa aatcgtgtcc cccagaataa tcaagaacgg ctgagacccc
     1201 accttatctc gaaccagaga cacggcatga cccaaaccca gcctcttttc ctgctcaaca
     1261 acgtgggtct tgaaggggta tttgtcccga acgtattcca cgatgagcct tccaagatgg
     1321 ccgacaatga gtacgacttc ctcaatgccc aaggattcca gtttgtccag gatatgcccc
     1381 agaatgggtt tgccagccac attgataaga actttgggcg tcgtgtgagt gagagggcgc
     1441 agacgcgtgc cttccccagc gacgggaatc actgccattc gaatacgact cggctctgaa
     1501 gtctgcttcg taccttctcc tgtcatatcc ttacccttcc agtcggtcag agaccgttcc
     1561 tcgcatcaag caaaaagaca gatcgtgctt agcatagcgc ctcggatatt gtcaagaatg
     1621 gcctcaagtt tttcgtggag tacacgcctt gttgcttgag ccggtgtatg ctatcgtcag
     1681 cgacaattct gaaacactgc gattgagagg aaacagaacg aggagacctt aggatgcagt
     1741 cggctcaaac cgctcagtca aataggattc ccgtcttctt tctgatcgcc gtggtcttga
     1801 ttgtcacaac gtgcctggcc gtggtggctt caccaaagcc gctggctgca atcggtgggg
     1861 tgctcgcttt tag
//
LOCUS       DAOQUC010000880         1871 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_80620_length_1871_cov_3.909692, whole genome shotgun sequence.
ACCESSION   DAOQUC010000880 DAOQUC010000000
VERSION     DAOQUC010000880.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1871)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1871)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1871
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80620_length_1871_cov_3.909692"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(260..1495)
                     /locus_tag="PKH07_13640"
     CDS             complement(260..1495)
                     /locus_tag="PKH07_13640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNT36025.1"
                     /translation="MRLSQKQLYEILNHVADLALVIDQNGRYLEIFTSREDLLYRPAS
                     ELIGKEFDEVLPEDKATQFKAVVREAIEQNRPISLDYELQTLGAMHWFCGTVCPLPSW
                     QDGRPAVVLFALDITERKNREARYTLENERMKQAQREAEKALREQEILAMRSDRLRSM
                     GEMAACMAHELNQPLSGIRGFAENVLISVQNGWEVTHEELIHKMNRIIQQTERMSNLI
                     NHVHDFSSDAERPVSMIVKASEVVKMALTMVSARFRAHSIDLKAFYQDEESVIRANPY
                     SLEEVLLNILSNAYDALQEKAKTDYGSSKIVEVVVKRQLSDNDEDADNVVIEVRDTGV
                     GMAPEQLQRAFEPFFTTKGPDRGAGLGLAVAKRTMDSLSGRLELLSKIGEGTTARLTF
                     PRFSSTTQHNHDADSAEES"
     gene            complement(1537..>1871)
                     /locus_tag="PKH07_13645"
     CDS             complement(1537..>1871)
                     /locus_tag="PKH07_13645"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNT36026.1"
                     /translation="HDVGDAVLQMVANTLSSNIRSFDRVIRWGGEEFLVILVNIQDSD
                     LPLIAEKIRVLVQKSFLKHQDISLSVTVSIGATLCRGDDTKESLLKRADSLMYDSKQA
                     NRNRVTIG"
BASE COUNT          413 a          525 c          448 g          485 t
ORIGIN      
        1 ttttacgaga aaatctcgaa gaggtcgttc tggaatcact tcgagatttt ctcgtaaaac
       61 tgggtcacct gaacacggtg ccccagtttt acgagaaaat ctcgaagtga ttccagaacg
      121 acctcttcgt cgtctacgat gagaatgttc cgatgtctta ctggcacgcg aaatgccccc
      181 tcatcctgcc tgtctccttg ctcggtctgt ccggattccg cgccgttgtt gaatccggcc
      241 ttgctctatc ctttatcgtt catgattcct ctgcactgtc tgcatcgtga ttgtgctgcg
      301 ttgttgaact aaatctcgga aaagtcaacc gcgcggtcgt tccttcacca atcttcgaga
      361 gcagttcaag acgccctgaa aggctatcca tcgttcgctt ggccaccgcc agacccaagc
      421 cagcaccccg atccggcccc ttggtcgtaa agaaaggttc aaaggcccgt tgcagttgtt
      481 cgggtgccat tccaacgccc gtgtccctca cctcaatgac tacattgtcc gcgtcttcat
      541 cattgtcaga caactggcgt ttgacaacaa cctcgacgat tttcgaagaa ccgtaatccg
      601 tcttggcttt ctcttgcagg gcatcgtaag cgttgctgag gatattcaga agcacttctt
      661 caagggaata agggtttgcc cgaatcaccg attcctcgtc ctgatagaag gctttcagat
      721 caatgctgtg ggctcggaat cgcgcggaga ccatggtcaa tgccatcttc acaacttctg
      781 atgctttgac aatcatgctg acaggtcgtt cagcatcact ggaaaaatcg tggacgtggt
      841 tgatgagatt gctcattcgt tctgtctgtt ggatgatgcg attcatcttg tgaatgagtt
      901 cctcatgggt cacttcccag ccgttctgaa cactgatgag aacattctcc gcaaatccac
      961 ggatgccgga cagcggttgg ttcagttcat gcgccataca ggcagccatc tcacccatgg
     1021 aacgcagccg atccgacctc atggccagga tctcctgctc acgaagcgcc ttttcggctt
     1081 cacgttgtgc ctgcttcatg cgctcattct caagcgtgta acgcgcttct ctgttcttgc
     1141 gttcggtaat gtcgagggcg aacagaacaa cggcaggccg accgtcctgc catgagggca
     1201 gtggacagac tgttccacaa aaccagtgca tcgcgccgag cgtctgaagc tcgtaatcca
     1261 gagagatcgg tctgttctgc tcgatcgcct ctcgaaccac cgccttgaac tgtgttgcct
     1321 tgtcttcggg aaggacttcg tcaaactcct ttccaatgag ttctgatgcg ggacggtaaa
     1381 gcagatcctc ccgactggtg aatatctcaa gatatcgccc gttctggtct atgaccaacg
     1441 ccagatcggc cacatgattg agtatctcat acagctgctt ctgactgagt ctcatctcgt
     1501 tcacaaggtc atccctttca cggcggtggt gccgtttcag ccgatcgtca ccctgtttcg
     1561 gttggcttgc ttgctgtcgt acatcaggct atcggcacgc ttcaaaagag actccttcgt
     1621 gtcatcacca cggcagagcg tggctccaat ggagaccgtc acactcaagg aaatatcctg
     1681 atgcttcaga aacgattttt gaaccagaac gcgaatcttc tcagcgatca aagggagatc
     1741 ggaatcctgg atattgacca gaatgacgag gaattcttct ccaccccaac gaatcacccg
     1801 atcgaaggag cggatgtttg aactcagcgt attggcaacc atttgaagta cggcatcccc
     1861 gacgtcgtgg c
//
LOCUS       DAOQUC010000881         1870 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_80697_length_1870_cov_3.229752, whole genome shotgun sequence.
ACCESSION   DAOQUC010000881 DAOQUC010000000
VERSION     DAOQUC010000881.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1870
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80697_length_1870_cov_3.229752"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            39..548
                     /gene="nusB"
                     /locus_tag="PKH07_13650"
     CDS             39..548
                     /gene="nusB"
                     /locus_tag="PKH07_13650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012097529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HNT36027.1"
                     /translation="MPTRRSARRVALQLLYQNDVAQCDVLETLEQVRYWPFQQINKLG
                     DDPSMDDSPDFLSSLHLEELDTQNLDYVLEVVRGTIEHRQEIDDMLREVSQNWRLERM
                     AVAERNILRMALYEILHRPEVPPRVAINEAIDLAKIYGDEKSGGFVNGVLDAAYLRFR
                     STSKSEENL"
     gene            556..>1870
                     /locus_tag="PKH07_13655"
     CDS             556..>1870
                     /locus_tag="PKH07_13655"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNT36028.1"
                     /translation="MTELSGNVLLVEDDQRYAERLKRNLELEGYQVTAALDGHEALTL
                     LENRSFDLVLTDLKMPGMDGLDLLRAMKSGEGTGVDPEVPVLVLTSVKSINSAVEAMR
                     LGAEDYVTKDCERDEILLRIRRAMEGRRMVTENRVLRERLQKQSEFGDVIAASAPMQA
                     VLHEVEEVASTEATVLVTGETGVGKELISRAIHDNSPRKQYPFIEVNCGALPNDNMFQ
                     SELFGHEKGAFTGAETMRKGRFELAHRGTLFLDEIGDLSPDSQRKLLRALELREFERL
                     GGTKRIRVDVRFVLATNRNLKEMADQGDFRKDLFYRINVYHIHIPPLRERRDDIAPQA
                     GYYLSFFAEKYGRTAREFSPEALKALEQCDWPGNTRELRIICERLAIRVSGSRVELEH
                     LALSGLGESEATTGRLVSLPSGGAGLDEIEREAIIQALERTDWVQK"
BASE COUNT          437 a          430 c          565 g          438 t
ORIGIN      
        1 tggagatgtc aagtctcatg gagcagattg gataatccat gcccactcgc cgatctgcac
       61 gcagggttgc tcttcagctt ctttaccaaa acgacgttgc tcaatgcgat gttctggaaa
      121 cactggaaca agtccgttac tggccattcc agcagatcaa taaattgggt gatgatccga
      181 gcatggatga ttctcctgac ttcctatcct ctctgcatct ggaggaactg gacacccaga
      241 acctggacta tgttctggaa gtggttcgag gaacgatcga acatcggcag gaaattgacg
      301 acatgcttcg agaagtttcc cagaactggc ggttggaacg aatggctgtc gcggaaagaa
      361 acattctgcg gatggctttg tacgagattc tgcaccgtcc ggaagtccct ccgcgggttg
      421 ccatcaacga agcaatcgat cttgccaaga tatatgggga tgagaagtcg ggtggttttg
      481 tcaacggcgt tcttgatgcg gcttacctga gattcagaag cacatcaaag agcgaggaga
      541 atctttgact ctatcatgac cgagttgtcc ggaaatgttc ttctggtcga ggacgatcag
      601 cgctatgctg agcgtttgaa gcggaacctg gagctggaag gctaccaggt tacggcggct
      661 ttggatggtc atgaggcgct tacattgctt gaaaatcgga gtttcgacct ggttctgacg
      721 gatctaaaga tgcctggcat ggacggtctg gatctgttgc gggcgatgaa gtctggtgag
      781 ggtacggggg ttgatccgga agttccagtt ctggttctga cttcggtgaa gtccatcaac
      841 tctgctgtcg aagcgatgcg ccttggcgcg gaagattacg tgaccaagga ctgtgagcgg
      901 gacgagattc ttctgagaat tcgacgagcc atggaaggtc gtcggatggt aacagagaat
      961 cgggtgcttc gcgaacggct tcagaagcag agtgagttcg gggatgtcat tgcagccagt
     1021 gctcccatgc aagcggtatt gcatgaagtc gaagaggtgg ccagtaccga agcgacagtt
     1081 cttgtcacgg gggagacggg tgtgggcaag gaactcatct ctcgtgccat tcacgacaac
     1141 agcccgcgca agcaatatcc gttcatcgaa gtcaactgcg gtgccttacc gaatgacaac
     1201 atgtttcagt ctgagctttt tggtcacgaa aagggggcat ttaccggtgc cgagacgatg
     1261 agaaaggggc ggtttgaact ggcgcatcgt ggaacactct ttctcgatga gattggggac
     1321 ttgtcaccgg actcacagag aaaacttctt cgtgctctcg aactgcggga gtttgaacgc
     1381 ctgggcggta ccaaaaggat tcgggttgac gttcggttcg tgcttgcgac gaatcgaaat
     1441 ctcaaggaga tggccgacca aggggatttc aggaaagact tgttctatcg gatcaacgtc
     1501 taccacattc acattccgcc ccttcgagag cgtcgagatg atattgctcc ccaggccggc
     1561 tattacctga gcttctttgc ggaaaaatat gggcgtaccg cacgtgagtt cagtcctgag
     1621 gctctgaagg cgctggaaca atgtgactgg cctggaaaca ccagggaact gcgcatcatc
     1681 tgtgagcgtc ttgccattcg ggtcagtggc tctcgtgtgg aactggagca tttggctctg
     1741 tcgggtctgg gtgagtcgga ggcgaccacc ggaagactcg tgagtcttcc ctcgggcggt
     1801 gcaggcttgg atgagatcga acgagaagca atcatccaag cgctggagcg cacagactgg
     1861 gtgcagaaaa
//
LOCUS       DAOQUC010000882         1865 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_81058_length_1865_cov_3.471271, whole genome shotgun sequence.
ACCESSION   DAOQUC010000882 DAOQUC010000000
VERSION     DAOQUC010000882.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1865)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1865)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1865
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81058_length_1865_cov_3.471271"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            134..1600
                     /locus_tag="PKH07_13660"
     CDS             134..1600
                     /locus_tag="PKH07_13660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36029.1"
                     /translation="MLLADMFVVIALLTLCRSAGASEISPEGPLTGPYPWRRLFLDAM
                     VVEESQGVKRVFHKAQKYAGNPIMVKDHPWEGVGPNTCAVIQEEGKFRMWYYGTYGGK
                     SCYAESTDGIQWTKPNLGLIEYEGSTANNICDFKGGVVMRVRKPVSPQSQYVVFGARK
                     ASPCVAFSADGLNWRWDEGKFDLYKTSDVMNYFYDPYKERYVATYKTPNRRHRAVGLA
                     YSENATDWYKPVEGAVFGADDYDPDGTQIYGMPVFPYQGLYIGMPWIYHARFFKYGEY
                     TSPQRMYEAETDSPRFIDTQMAWSWNLISWNRTVKRKPFLELGAPGSWDCGMVLMRGA
                     PIPVGDKLFFYYAATDRIHDVVKDVRCCIGIATLRLDGFCSLRARDKEGWVISRREVF
                     KAPRVTLNAKTRQDGYVVAELLDRNNNVVEGFSREACIPFQGDSVNATLSWKSTEFPK
                     EMLEPDKKIRFFFRNADLYSYLPEDIDTEQDTSNRKEH"
BASE COUNT          445 a          464 c          530 g          426 t
ORIGIN      
        1 tgcccagagg agtccagttt gggtacccat agaaggatgg tgcagatggg ctccaatagg
       61 tcctcagtat cggtgatcgg tgcgatactg acggaacgtc cctatcatgg tcatgaacca
      121 aggagcaatc gcaatgttgt tggcagatat gtttgtcgtc atcgctctat tgactttgtg
      181 ccgaagcgcc ggtgcatcgg agatctcccc tgaagggccg ttgacaggcc cctatccctg
      241 gagacgactg tttctggacg cgatggtggt tgaggagtct cagggagtca aacgggtctt
      301 ccacaaagcg cagaagtatg cgggcaatcc catcatggtc aaagaccatc cgtgggaagg
      361 agttggaccc aatacctgtg ccgtgataca ggaagagggc aagttccgga tgtggtacta
      421 tggaacctat ggggggaaga gctgctacgc ggaatccaca gatgggatcc aatggacgaa
      481 acctaatctc ggtctgatcg agtacgaagg ctcgaccgca aacaacatct gcgatttcaa
      541 gggtggcgtg gtcatgcggg ttcgcaaacc ggtatctcca cagtcacaat acgttgtgtt
      601 tggagccagg aaagccagcc cgtgtgtggc gttctccgca gacggactca actggcgttg
      661 ggacgaaggc aaattcgatc tctacaagac gtccgatgtc atgaactact tctacgatcc
      721 ctacaaggaa cgatacgtcg ccacatacaa gacgcccaat cggcggcatc gagctgtcgg
      781 gcttgcgtat tcggagaacg ccacagactg gtacaagccg gtcgagggtg ctgtcttcgg
      841 tgcggatgac tatgacccgg acggcacgca aatctatgga atgccggtct ttccgtatca
      901 aggtctctac atcggaatgc cgtggattta ccatgcccgt ttcttcaagt atggggagta
      961 cacgtctcct cagcggatgt acgaagcaga aaccgattcg ccgcgattca ttgacacaca
     1021 aatggcatgg agttggaatc tgatcagctg gaatcgaacg gtcaagagaa agccttttct
     1081 ggaactgggc gcacccggaa gttgggactg tggcatggtc ttgatgcgcg gtgctccgat
     1141 tcccgttggt gacaagctgt tcttctacta cgcggccaca gaccgcattc atgatgtggt
     1201 caaagacgtc cggtgctgca tcggaatcgc aacgctgcga ctggacggtt tctgttcctt
     1261 gagggcacgc gacaaagaag gttgggtcat cagccgtcgt gaggtcttca aggcaccgcg
     1321 cgtgacactg aatgcaaaga ccagacagga cggctacgtt gtggcggaac ttctcgatcg
     1381 aaacaacaat gtagttgagg ggttttcgag agaggcatgc attccctttc agggagattc
     1441 cgtgaacgca actctatctt ggaagtcaac ggagtttccc aaagaaatgc tggaaccgga
     1501 caagaaaatc cgtttcttct tccgcaatgc cgatctgtat tcctacctgc cagaggatat
     1561 tgacaccgag caggacacca gcaaccgaaa ggaacactga tcggtcacca ggctccacag
     1621 gttctccgat gtggcaggga acggctctgc tgcaaatggt cactgttctt tcatcgcctt
     1681 gacttatatg gcatctcttc tctattcttc ccttcgtctg gagatcgcga atcgttcgtg
     1741 atcagaagag agataatgga cgggttgccc acagccaatc tgctgtttga gtcattgacg
     1801 cctctcaaat gcagagtgcg cgtttcgcgt ttgcgttggg agatgatcgt tgagacaaag
     1861 catcc
//
LOCUS       DAOQUC010000883         1865 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_81063_length_1865_cov_3.249724, whole genome shotgun sequence.
ACCESSION   DAOQUC010000883 DAOQUC010000000
VERSION     DAOQUC010000883.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1865)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1865)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1865
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81063_length_1865_cov_3.249724"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1271)
                     /locus_tag="PKH07_13665"
     CDS             complement(<1..1271)
                     /locus_tag="PKH07_13665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36030.1"
                     /translation="MATEWLERKRKELAKAEEKENDQATETPISWNPDPGGDFDGFEQ
                     EIRQAAQHRNGEDISDALRVYLWGYVDQQIRDACDKVGIVPEGRSCHFLSHSAGTWFR
                     LPRSLNVTPDSFGKAFFQENIYAKFYDNYFREIPKELLVTLGDFFVQRPGAAHGTVQL
                     EAFDSPTLVVIADKDVLSHCATPIDDLRKYDLATVDGKATLQVVALEEQTISSVGKEV
                     FTKDKDSFQALIRSCHRFLSDRVAFLADVEAEKKSPICKALYSAMENFTDQKMFEKLQ
                     ITERGDQLEITLAFLLSFYSALTTDFERVYAAYQTHKKYDYKSRLLGLYVDPIKASFT
                     NRKKRGAFFVGFSCEEQDFLTRAKVVISDVHDLFSRETKRLYGLDSQIALETADGQSR
                     ILLEEMLVRCSLISDKHKEDLSVNTARRAVL"
     gene            complement(1261..>1865)
                     /locus_tag="PKH07_13670"
     CDS             complement(1261..>1865)
                     /locus_tag="PKH07_13670"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNT36031.1"
                     /translation="NFSQDNFRRLYEAEGGTVTGAEAYPPGATDFRSQLTRLAQASPD
                     VLYFLSYSEWATIEKQARELGMKCDFAGATPTEDAKALGIAGDVANGTIYTRAAFDPN
                     AVAPVLEEFQRQYRQKYGTTAEVWAATFRDNVLLVARAAAEGAKTDAALIDKIVSFKT
                     YQGASGETEFLANRDTNKPVSIFAIVQGKFQPLGRRNHGN"
BASE COUNT          403 a          528 c          480 g          454 t
ORIGIN      
        1 ttcagaacag cgcgacgagc tgtgttgaca ctgagatcct ctttgtgctt gtccgaaatc
       61 aggctgcagc gaaccagcat ttcctcgagg agaatgcgac tctgtccgtc cgcggtttcc
      121 agagcgatct gagaatcgag cccatagagg cgtttggtct ctcgggagaa gaggtcatgg
      181 acatctgata tcacgacttt ggcccgagtg aggaagtcct gctcctcaca ggagaaaccc
      241 acgaagaaag cgccacgttt cttgcggttg gtgaaacttg ccttgatggg atctacgtat
      301 aatccgagga gccgggactt gtagtcatac ttcttgtgcg tttggtatgc agcatataca
      361 cgctcgaaat cagtcgtgag agcagagtag aaactcagca agaaggccag tgtgatctcc
      421 agttggtcgc cccgttccgt gatctgaagc ttttcaaaca tcttctgatc tgtgaagttc
      481 tccatggccg agtacaaagc cttgcatatc gggctttttt tctcagcctc cacatccgcg
      541 agaaatgcaa cgcgatcaga gagaaaacgg tgacaactgc gaatcagcgc ctggaacgag
      601 tctttgtcct tcgtgaatac ctccttcccc acggagctta tggtctgctc ctccaaagcc
      661 acaacctgca gtgttgcttt tccatcgaca gtggccaagt catacttcct taggtcgtca
      721 atcggcgtcg cacaatgaga cagcacgtcc ttgtctgcaa tcacaacaag agtagggctg
      781 tcgaatgcct caagctggac tgttccatgt gccgcgccgg gccgttggac aaagaagtca
      841 cccagggtga caaggagttc cttgggaatc tccctgaagt aattatcgta gaacttagcg
      901 tagatattct cctggaagaa cgctttcccg aatgagtccg gcgtgacgtt cagagagcgg
      961 ggcagccgga accatgtccc tgccgaatgg gacagaaaat gacaactgcg accttcaggg
     1021 acaatcccca ccttgtcaca tgcgtctcgg atctgttggt cgacatagcc ccagagatac
     1081 acccggagag catcggagat atcctcgccg ttgcgatgct gcgctgcttg gcgaatctcc
     1141 tgctcaaagc catcaaagtc accgcccgga tcaggattcc acgaaatggg agtctctgtt
     1201 gcctgatcgt tttccttctc ctcggccttc gccagttctt ttcgcttcct ctcaagccat
     1261 tcagttgcca tgattgcgtc tccccagcgg ctggaatttg ccctgaacga tggcaaagat
     1321 actgaccggt ttattggtat cgcgattggc gagaaactcg gtctcaccac ttgccccttg
     1381 gtacgtcttg aagctcacga tcttgtcgat caacgcggcg tctgtcttag caccctccgc
     1441 agcggcccgc gccaccagca ggacattgtc ccggaacgtg gctgcccaga cttccgccgt
     1501 ggttccgtac ttctgccggt actgacgttg aaattcctcg agaaccgggg cgaccgcgtt
     1561 gggatcgaaa gccgcccggg tgtagatggt cccatttgct acatcgccgg caatcccaag
     1621 cgctttggca tcctctgttg gagtcgctcc cgcaaaatcg cacttcatgc caagctctct
     1681 ggcctgtttc tctatcgttg cccattcgct gtatgagagg aaatagagta catccggact
     1741 tgcttgtgct agcctggtca actgtgatcg gaaatccgtt gcgccgggtg ggtaggcctc
     1801 tgccccagtc acagttcctc cctccgcttc atacagcctt ctgaaattgt cctgagagaa
     1861 attgc
//
LOCUS       DAOQUC010000884         1864 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_81128_length_1864_cov_3.632394, whole genome shotgun sequence.
ACCESSION   DAOQUC010000884 DAOQUC010000000
VERSION     DAOQUC010000884.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1864
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81128_length_1864_cov_3.632394"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..900
                     /locus_tag="PKH07_13675"
     CDS             <1..900
                     /locus_tag="PKH07_13675"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HNT36032.1"
                     /translation="SPLPESSTTEESSTTIWESSTGDSNTVPSSVAESTGTLEVGGQS
                     PTDFAEMIRQAQEELERDRVLRPLRAGSFVTRIVLDPGHGGGDNGSVGPSGLKEADVC
                     WQVCQLLKSRIEQSTNLEVFLTRRMDEGGAISNSVRVARANSAQGHLLVSLHCGAGLF
                     PQQSGQTVFYPSAQAGDENQSESQAMEILSGADVGQQSTLIPWKLAGRPYQSATVRLA
                     GKIYERMRIWMTEREEVPRAAFIELLQSVQMPAVLVELGVISNPKDEEKLRQKGYQKE
                     LAEALYMGITNFIREQEQYAGQQ"
     gene            884..1564
                     /locus_tag="PKH07_13680"
     CDS             884..1564
                     /locus_tag="PKH07_13680"
                     /inference="COORDINATES: protein motif:HMM:NF022112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HNT36033.1"
                     /translation="MPVNSSTPRNSRTEERLLIALVALLVCLVLLMIAGGGFLYFQIR
                     SDKQVTLPPGMVSHRTPDQTQDVPLSGEEIQVFFLSPADMKLVGEPRRIHDSKTLVER
                     ATRALEALLTGPETADLLATIPDQSRLHAVYHDPEARLLYVDFAATFYRNLPGHSLAE
                     WAAIYSVVNTLCSLTEEIQAVVFLEDGKPLSSSPGNWNWDQAFQPDKAWVRYSTPGDE
                     QAVEQEQP"
     gene            1611..>1864
                     /locus_tag="PKH07_13685"
     CDS             1611..>1864
                     /locus_tag="PKH07_13685"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011358381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HNT36034.1"
                     /translation="MNFNQKVQKEWNRESRSLLGYALKAASFGYGLAVGLRTQTFASG
                     IRETHTLPVPVVCVGNLVVGGVGKTPLVEPVARFYLEIGL"
BASE COUNT          423 a          512 c          528 g          401 t
ORIGIN      
        1 agtcctttgc ctgagagcag caccaccgag gaatcgtcaa caaccatttg ggaatcttcg
       61 acaggggatt ccaataccgt cccgtcctct gtcgcagagt caacaggcac tctggaagtg
      121 ggtggccaga gccctacaga ttttgcggag atgatccgtc aagctcagga ggaactcgaa
      181 cgagacaggg ttcttcgtcc cctgagagcg gggagttttg tgacacggat cgtcttggat
      241 cctggtcacg gtggaggaga caacggctcg gttggacctt caggcttgaa ggaagccgat
      301 gtctgctggc aggtctgtca attgctcaag tctcgtatcg agcaaagcac caacctggag
      361 gtttttctca cgcgtcgaat ggatgaaggg ggagcgatca gcaattctgt ccgtgtcgcc
      421 cgagccaact ccgcacaggg gcatcttctc gtcagtttgc attgcggggc gggactcttc
      481 ccgcagcagt ccggtcaaac agtcttctac cccagcgccc aggccggaga cgaaaaccag
      541 tcggaatctc aagccatgga gatcctctcc ggagcagacg tcgggcaaca gagcactctc
      601 atcccctgga aactggcagg gagaccctat cagtcggcaa ccgtgcgact tgccggaaag
      661 atatacgaac ggatgcgcat ctggatgaca gagagagaag aggttccacg tgcagccttc
      721 atcgaactgc ttcagtcggt gcagatgcct gcggtgctgg tggaactggg cgtgatttcc
      781 aatcccaaag acgaggaaaa actccgccaa aaaggctatc aaaaggaact cgcggaagct
      841 ttgtatatgg gcatcacaaa cttcattcgg gagcaggaac aatatgccgg tcaacagtag
      901 tacgcccaga aactccagaa ctgaggaaag gctccttatt gcgcttgtcg cccttctggt
      961 atgcctcgtg cttctgatga tcgccggggg cggcttcctc tattttcaga ttcgatccga
     1021 taagcaggtg actctgcctc cagggatggt gagccaccgc acgccagatc agacacagga
     1081 tgttcctctt tcgggagagg agattcaggt gttcttcctc tctcctgccg acatgaaact
     1141 ggtgggagag ccgcgcagga ttcacgactc caagacactg gtggagcgtg caacccgagc
     1201 gctggaagcc cttctgacag gtcccgaaac cgcagacctt ctggccacca tccccgacca
     1261 aagcaggtta catgcggtgt accacgatcc agaggctcgc cttctgtatg ttgactttgc
     1321 tgcgactttc taccgcaatc ttccaggtca ctcccttgct gaatgggcag cgatctattc
     1381 ggtcgtcaat acactatgct cgctgacaga agagattcag gcggtcgtct tcctcgaaga
     1441 tggcaagcca ctttcatcgt ctcctggaaa ctggaactgg gatcaggctt ttcagccgga
     1501 caaggcgtgg gttcgctatt cgactcccgg cgatgagcag gccgtcgaac aggagcaacc
     1561 ctgaacttgc gcataacaag gcgacggcat tcccttcaca ggtgccctgc atgaatttca
     1621 accagaaggt acagaaggaa tggaaccgtg agagtcggag cctgctggga tacgctctca
     1681 aggcggcttc ttttggttac ggcctcgcgg ttgggcttcg cacgcaaact ttcgcgtcag
     1741 gaattcgaga gacccacaca cttccggttc cggtcgtctg cgttgggaat ctggttgtgg
     1801 gcggagtggg aaagacacca cttgttgagc ccgtagcgcg cttctatctc gagatcggct
     1861 taat
//
LOCUS       DAOQUC010000885         1862 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_81269_length_1862_cov_3.038185, whole genome shotgun sequence.
ACCESSION   DAOQUC010000885 DAOQUC010000000
VERSION     DAOQUC010000885.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1862
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81269_length_1862_cov_3.038185"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..832
                     /locus_tag="PKH07_13690"
     CDS             <1..832
                     /locus_tag="PKH07_13690"
                     /inference="COORDINATES: protein motif:HMM:NF022623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HNT36035.1"
                     /translation="GDFFCHALGHKTAFENACFSSPEGRSFNCFIRMPFRKAARIVLV
                     NESGKDNGVYYEVDATVGDRQDKNTLYCHAHWRRENMTTLRQDMTILPQITGWGRFLG
                     CSLGVRLHPSCTNFWWGEGEVKIYLDGDTQLPTLCGTGTEDYIGTGYGQGLFDHMYSG
                     NPYISKDRDAYGFYRFHIPDPVYFRESLRVTIQVMGGPSYRQMLEALDKDPSLRFMKA
                     GDGTQYYTREELEAQPDRAEVMERIDDHCATAYWYMNTAENGLPPIAPFEDRIKDIPL
                     "
     gene            complement(1326..>1862)
                     /locus_tag="PKH07_13695"
     CDS             complement(1326..>1862)
                     /locus_tag="PKH07_13695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36036.1"
                     /translation="AKFNRLSKNAREGERFVANWLCAGFNSEAKMKTKAPAKKKAQKP
                     SCGLCGKTENLTKTECCGNWICDDEHKYVLFSYATNSCYRNHRRCTLCGFHFSEGHRG
                     SWKTCKTCRSSFEPEMVVWYGTNEFNFEKMPDPPSYEPTKCSLCGAVIRLGFENHSRA
                     EGEYWCEACTEKTFKTRG"
BASE COUNT          430 a          480 c          461 g          491 t
ORIGIN      
        1 cggtgatttt ttctgtcatg cccttgggca caagacggcc tttgagaatg catgtttctc
       61 ttcaccggaa ggccgttcct tcaactgctt tatccggatg cccttcagga aggcggcgag
      121 aattgtcctg gtcaatgaat caggcaaaga caacggcgtc tactatgaag tggacgccac
      181 agtcggggat cgccaggaca agaacacact ctattgtcac gcgcactgga ggcgggagaa
      241 tatgacgact cttcgccagg acatgaccat cctgccgcaa atcacgggtt gggggcgctt
      301 tcttggatgc agtcttggcg tcaggttgca cccttcctgc accaatttct ggtggggtga
      361 aggcgaagtc aaaatctacc tcgacgggga cactcaactg ccaacactct gcggcactgg
      421 aaccgaagac tacatcggaa ctggatacgg acaaggtctc tttgatcata tgtattcagg
      481 caacccgtac atttccaaag acagagatgc ctatgggttc tatcgttttc acattcctga
      541 tcctgtctac tttcgagagt cgcttcgtgt gactatccaa gtcatgggtg gccccagcta
      601 tcgccagatg ctggaggcgc tggataagga tccaagtctt cgcttcatga aagctggcga
      661 cggcacccag tactacacac gcgaggaact tgaagcacag cccgatcgtg ccgaagttat
      721 ggaacggatt gatgatcact gcgctactgc atattggtac atgaatacag cggagaatgg
      781 gcttccccca attgcacctt tcgaggatcg gatcaaggac attcctctct aggacgaatc
      841 atcacaaggc gccagtcctt tgttgtgtgg agttgaattg gccaaataac cggctggatc
      901 tcagtcctag agaacattca ttaaccttgc atgatatatc gatgctccta gcccaacttc
      961 cgcagtttgg ggtgcatttt tttgaaagaa ggtttttgct tgtgagatgg ggaccttttt
     1021 cgtgtgtttt cgggtatttt tcctgttcct gtgatggctg tgtggtctta ttggatgagt
     1081 gaatgctgaa aagtgcgtgt agtgaagagt caatctgcca acgtccattg cattaccttt
     1141 aacgtgcaga ggaactggcg ggaatgaccc gccataccgc ttgggactta ccgcgaacta
     1201 atttagatga tgcccgccca tgcgtcaatg agaccagaga cctgaaagcc agcaggaacg
     1261 tcgtaccgtg ctgttagaaa gtaattcctg atggcacgga tgaacgccca tttccgttct
     1321 ttggttcaac cccgagtctt gaatgtcttt tctgtacatg cctcgcacca gtattcgcct
     1381 tccgctctgc tgtgattctc gaagccaagt ctgatgacgg ctccacagag cgagcatttt
     1441 gtcggctcgt atgaaggcgg atccggcatc ttctcgaagt tgaactcgtt cgttccatac
     1501 cagacgacca tctccggctc aaaggaagac cgacacgtct tgcaggtctt ccaactaccc
     1561 ctgtgacctt cgctgaagtg aaagccacac agcgtgcacc gacgatggtt gcggtaacaa
     1621 ctgttggtcg cgtatgaaaa gagtacgtac ttatgttcat cgtcgcatat ccagttacca
     1681 cagcattcgg tcttcgtcag attctccgtt ttcccacaaa gaccacaact tggcttttgc
     1741 gccttcttct ttgctggtgc ttttgttttc atcttagcct ccgaattaaa tccagcacac
     1801 aaccagttgg caacaaaacg ctcaccttcg cgagcgttct ttgatagcct gttgaacttc
     1861 gc
//
LOCUS       DAOQUC010000886         1862 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_81275_length_1862_cov_2.835086, whole genome shotgun sequence.
ACCESSION   DAOQUC010000886 DAOQUC010000000
VERSION     DAOQUC010000886.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1862
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81275_length_1862_cov_2.835086"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..406
                     /locus_tag="PKH07_13700"
     CDS             <1..406
                     /locus_tag="PKH07_13700"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNT36037.1"
                     /translation="AEFYEGAVRLAPADGAVWYNMGLYWQRAGLLKEAISAYRQAISR
                     DAELAYAYNNLGLCLMLTGKVREAGEVLNAGLQKDQRSPDIHFNLALVYRTIGDNAKA
                     ESYLERAKALAPERKHIQVTVEELDDWLERMH"
     gene            complement(478..1770)
                     /gene="serS"
                     /locus_tag="PKH07_13705"
     CDS             complement(478..1770)
                     /gene="serS"
                     /locus_tag="PKH07_13705"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013910402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HNT36038.1"
                     /translation="MLDLKKIRENPEGIEIQLRRRDPSISLKEILSLDERLRVRKSSL
                     DDMRNKLNTASTEIGRRKKAGESADELIRDMSSLSEQIKEAESEEKKMQTDLFCRLSF
                     LPNLPHPSVPTDMNKAHNVVVRKSGTQVEFDFSFKNHLEISRTLGILDFERGAKVAGS
                     QFPLYVGWGALLEQALLMFMLHTNASKGYRPILPPYLINEQTMFASGNLPKFEEQLYR
                     CRDDDFFLLPTSEVPLTGLHRDEILDEQQLPIRYTSMTACFRREAGTYGSEERGLVRV
                     HQFNKVEMYKIVTPESSYDELESLVADAEDLCRLLNLHYRTSLLVTGDIGNQSAKTYD
                     VEVWLPGQNAYYEVSSCSNCEDYQARRGNIRCRPGGSGGKGKLRHPHTLNGSGLATSR
                     LMVCILESNQQPDGSVVVPEVLRPYMMGREILEPARGL"
BASE COUNT          428 a          475 c          493 g          466 t
ORIGIN      
        1 agccgaattt tacgagggtg cggtcaggct tgctcctgct gacggtgcag tttggtataa
       61 catgggtctt tactggcaga gggctggcct tctcaaagag gcgatctcgg cataccggca
      121 ggcaatcagc cgtgatgcag aacttgccta tgcgtacaat aatctggggc tctgcttgat
      181 gttgacggga aaagttcgcg aagcgggtga ggttctaaac gctggcctgc agaaggatca
      241 acgaagcccg gacatacact tcaatcttgc tctggtctac agaaccattg gcgacaatgc
      301 caaggcagag tcctatctcg aacgtgccaa agcccttgcc ccggagagaa agcatatcca
      361 ggtaaccgtt gaggaactgg atgactggct tgaacggatg cattgaggat tcggcgtcgc
      421 acacgagcgg ccgaattgac atcaatgtgt tacgcctgcc agacaataag gcctctgtca
      481 gagacctctt gcaggctcaa gaatctcccg ccccatcatg tacggtcgca agacctcagg
      541 aacaacaaca gacccgtctg gctgttgatt gctttcgagt atgcatacca tcagtcggga
      601 tgttgccaga ccggaaccgt tcagtgtgtg tggatgacgc agtttgcctt tgccacctga
      661 tccgccgggt cggcatcgga tgtttcctcg tctggcctga tagtcttcgc agttgctgca
      721 ggatgagact tcatagtagg cattctgtcc cgggagccaa acctcaacgt cataggtttt
      781 cgccgattga ttgccgatgt caccggtgac caacaatgag gttctgtagt gcagattcag
      841 gagacggcag agatcttcgg cgtccgctac aagtgattcc agttcgtcgt aggaggattc
      901 cggagtgact atcttgtaca tctcaacctt gttgaactga tggacgcgaa ccaaacccct
      961 ttcctcggag ccataggttc cagcttcccg tcgaaaacac gctgtcatgg aagtgtatcg
     1021 gatcggcagc tgctgctcat ccaggatctc gtctcgatgc agtccggtca acggtacttc
     1081 tgaagtcgga agcagaaaga aatcatcgtc acggcaacga tagagttgct cttcaaactt
     1141 tggcagattt cccgaggcaa acatggtctg ctcgttgatc aaatagggag gcaggatcgg
     1201 tctgtatcct tttgacgcat ttgtgtgaag catgaacatc agcagcgcct gctcgagaag
     1261 tgcgccccaa cccacataga gagggaattg cgagcccgca acctttgcgc cgcgctcaaa
     1321 gtccagtatt cccagcgttc tgctaatctc cagatgattc ttgaaggaga aatcaaactc
     1381 aacttgcgta cccgatttgc gaacgacaac attgtgtgcc ttgttcatgt ccgttgggac
     1441 gctgggatga gggagatttg gtaggaaact gagacggcag aacagatctg tctgcatctt
     1501 cttttcttcc gactccgcct ccttgatctg ttccgagaga ctggacatgt ccctgataag
     1561 ttcatcggca gactcaccgg ctttcttgcg tctgccgatt tcggttgagg cggtattgag
     1621 tttgttgcgc atatcgtcaa gagatgactt gcgcacgcga agcctttcat ccaatgaaag
     1681 aatctccttc agggagatcg atgggtcgcg acggcgcaac tggatttcaa taccttcagg
     1741 gttctctcga atcttcttca agtccaacat gaatctcttg cctccagagg tgccttgtgc
     1801 atattgggga ggatactaca acgagttcgt gctgacttca aatctttgca ttttcccttt
     1861 gc
//
LOCUS       DAOQUC010000887         1858 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_81531_length_1858_cov_3.236273, whole genome shotgun sequence.
ACCESSION   DAOQUC010000887 DAOQUC010000000
VERSION     DAOQUC010000887.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1858
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81531_length_1858_cov_3.236273"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            348..1592
                     /gene="icd"
                     /locus_tag="PKH07_13710"
     CDS             348..1592
                     /gene="icd"
                     /locus_tag="PKH07_13710"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013683979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate dehydrogenase (NADP(+))"
                     /protein_id="HNT36039.1"
                     /translation="MTFDKLIEPKEGVRVQSEKGCLSVPDNPIIPYIEGNGIGTDIMR
                     ATHLVLNAAVSAAYGGNKRLCWFEIYAGEKAQAKYGEWLPQDTFKAIEYYRVALKGPL
                     TTPVGGGFRSLNVTLRQVLDLFACVRPVRYFQGVPSPVREPEKMNVVIFRENTEDVYA
                     GIEWPEGSPEATKLIALLKEQFNVEVRPDSGIGIKPISITGTQRLVRSAIRYAIARGR
                     KSVTLVHKGNIMKYTEGAFRDWGYQLAATEFADQTISEKEVTEKHQGKTPSDKILIND
                     RIADSMFQQILTRTDEYEVLATPNLNGDYLSDACAAQVGGLGIAPGANFGDDVALFEA
                     THGTAPKYANQDKVNPGSLILSAVMMLEHLGWQEAADKVVRGFETTLLKKQVTYDLER
                     LMDGAKLLKCSEFAQAIVDNMP"
     gene            1694..>1858
                     /locus_tag="PKH07_13715"
     CDS             1694..>1858
                     /locus_tag="PKH07_13715"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545039.1"
                     /note="Catalyzes the reversible oxidation of malate to
                     oxaloacetate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HNT36040.1"
                     /translation="MRPKVSIIGAGNVGATCALYLAQSQVADVVLLDIVEGMPQGKAL
                     DIQESCLPLGI"
BASE COUNT          453 a          457 c          525 g          423 t
ORIGIN      
        1 ctggaggaac tcattctccc gctcctggct gatcgtttct ggagagaaac cagcggccag
       61 aatccggtaa aagtgcatcc agatgcgcat caactcctgg atttcctcta tcttgtgctc
      121 taagactgga tcacgcactg caaatactcc ctatttttct tgcgctcttc ccgcacgagt
      181 ttcgaattgg cttgtagaat aacttgttgc attatagaat cgcccccgca actgtcaaga
      241 taattgcggg tgtgcgtcgg taaagagggt cgagattcag ttttgtgcaa cagctcaccc
      301 aaaggggatc ttccccaagg tggcagtcta tctgaggagg aacggcaatg acatttgata
      361 agttgatcga acccaaggaa ggcgtgcgtg tccaatccga aaagggatgt ctgagtgttc
      421 ccgacaaccc catcatcccg tacatcgaag gaaatggcat cggaacagac atcatgcgtg
      481 ccacacattt ggtactcaac gcagcggtgt cggcagccta cggtggcaac aagaggcttt
      541 gctggtttga gatttatgcc ggcgagaaag cacaggcgaa atacggcgaa tggctgcccc
      601 aggatacgtt caaggcgatt gaatactacc gtgtggcatt gaaggggcct ctgactacgc
      661 cggttggtgg gggcttccga agtttgaatg tgacgctgcg tcaagttttg gatctgtttg
      721 cctgcgtcag gccagttcgc tatttccaag gcgtgccttc tcccgttcgg gaaccagaga
      781 agatgaatgt cgttattttt cgtgagaaca ccgaagacgt ctatgctggc attgaatggc
      841 ctgaaggttc tcccgaagcc acgaaactga tcgcattgct caaagagcag ttcaacgttg
      901 aggtcagacc ggattccggt atcggcatca aaccgatatc tatcaccgga acgcagcgct
      961 tggttcgatc cgcgattcgg tatgccattg cccgtggccg aaaatctgtc acgctcgttc
     1021 acaaaggtaa catcatgaag tacaccgaag gtgcctttcg ggactgggga taccagttgg
     1081 cagcgacaga gttcgccgat cagaccattt cggagaagga agtgactgaa aagcaccagg
     1141 gcaagacccc ttcggacaag attctgatca acgaccgcat tgcggactcc atgttccaac
     1201 agattctgac ccgaacagat gaatatgagg tgcttgcgac accgaacctc aatggcgact
     1261 atctttcgga tgcctgcgcg gcgcaggttg gtgggctggg catcgctccc ggagcgaatt
     1321 ttggcgatga cgttgccttg tttgaagcca cgcatggcac ggcgcccaag tatgccaacc
     1381 aggacaaagt caacccaggc tccctgattc tcagcgcagt catgatgctg gagcatctcg
     1441 gatggcagga ggccgcggac aaggttgtcc gaggatttga aaccacactg ctcaagaagc
     1501 aagtcacgta tgatctggag cggctcatgg acggagcaaa actgctcaag tgttctgagt
     1561 ttgcacaggc gattgtggat aacatgccct gacttggatc ggacacacgg gcagaacctc
     1621 tcctcgcgag gcgcttgttc gagagaagaa agcgtcctct gggagtaagg ttgcccagga
     1681 ggaatggaga aggatgagac ccaaagttag tattattgga gcgggaaatg taggagctac
     1741 ctgtgctctg tatcttgcac agtcacaggt ggcagacgtt gtgttgttgg acatcgttga
     1801 agggatgcct caggggaaag cgctggacat tcaggaatca tgcctgccct tgggtatc
//
LOCUS       DAOQUC010000888         1856 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_81695_length_1856_cov_2.876180, whole genome shotgun sequence.
ACCESSION   DAOQUC010000888 DAOQUC010000000
VERSION     DAOQUC010000888.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1856
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81695_length_1856_cov_2.876180"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            24..>1856
                     /locus_tag="PKH07_13720"
     CDS             24..>1856
                     /locus_tag="PKH07_13720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36041.1"
                     /translation="MFLPSDLMPLSSTNLSGVALYRDRKNAGTVGAYDPGDIFVPISA
                     TDLEWNADPSDPIVRNGGHYMVLKPTSGQDVPDSDYPTSDSNWAAFGYDYFVCIRTSK
                     NIDYHDGFRCFIRDGDVKFRNSRNATGSGIDGDPIAANVPTFLNNLVVAPAGQDAMPI
                     RADSVATAVVGMDMHDSNRTFEGRPSKLSQVVVHLFNFGTDTDFTSTDLAPFYQGYGQ
                     WVEIDPRTGATIPFTGGNVAGKRIGSRCGVALYRDAPNSDTPGGFDNPFDLNVTNPDT
                     PVLLSGYEDWNFLLGWTYQVVLDLDPPMYDGTGADTSLVDPFDPEFYETLPAVDETEG
                     LGNDFFVVLKTSPTISYGDDFYVRVGSTSMGGWVLQPLYFFPYLLNSGTHPFPWTPPF
                     NAAWSFEDVNTQLMRCPTITITSFRDLTAKDNRVDATSDPIPVFGFDYHADTLSRETV
                     NAVRVQARNISGFTPQMDLRPILGGLDGLSGVELYRDGNGNGVWDTTDVRVAIKASQW
                     ENVYYADNPGPDGVLATSDDLGTLGVYDPGTDELFLDDGFITGYYDLGDTLIEDGTDN
                     IRHTLPTSYDIQDATLYALPSGQERWSLLMVPQTPIPVPQAFDGI"
BASE COUNT          397 a          469 c          536 g          454 t
ORIGIN      
        1 ctgagagcct tgagggagac ggggtgttcc ttccgagtga cctgatgcct cttagcagca
       61 ccaacctgag tggtgtagcg ttataccgag acaggaaaaa cgcagggact gtgggtgcct
      121 atgatcctgg ggacatcttt gtgcccatct cggcgacaga tctcgaatgg aacgccgacc
      181 catcggatcc gattgttcgc aatggcggac actacatggt gttgaaaccc acctcggggc
      241 aggatgtccc cgacagtgac tatcccacca gcgactcgaa ctgggcagct ttcggctatg
      301 actactttgt gtgtatcaga accagtaaga acattgacta tcacgatggg ttccgatgct
      361 ttattcgaga tggggatgtc aagttccgca attcgcgcaa cgcgaccggt agtggcatag
      421 acggggatcc gattgccgcc aacgtgccga cgttcctgaa caacctggtt gtggctccgg
      481 caggacaaga cgccatgcca atccgagcag acagtgttgc gacagcagtt gtcgggatgg
      541 atatgcacga ctccaatcgc acgttcgaag ggagaccttc caaactgtct caggttgtcg
      601 tccatctgtt caactttggt acagacacag actttacatc aaccgatctt gcacctttct
      661 atcaggggta tggccagtgg gtggagattg atccgaggac tggggcgaca attccgttca
      721 ctgggggtaa cgtcgccggc aaacgtatcg gttcgcgttg tggcgttgcg ctctatcgcg
      781 atgcacccaa tagcgatact ccaggtggat ttgacaatcc ttttgacctg aacgtaacca
      841 acccggacac gccggtattg ttgtcaggtt atgaggactg gaacttcctg cttggatgga
      901 catatcaagt ggtgttggat ctggatccgc ccatgtatga tgggaccggt gctgacacaa
      961 gtcttgtgga tccctttgat cctgagttct atgagacgct tcctgccgtg gacgagacag
     1021 agggtctggg gaacgatttc tttgttgttc ttaagaccag tccgaccatt tcttacggag
     1081 acgatttcta tgtacgggtt gggtctacga gtatgggggg atgggttctg cagccacttt
     1141 acttcttccc ctatcttttg aattctggga cgcatccttt tccgtggacg cctcccttca
     1201 atgcagcctg gtcttttgag gatgtcaaca cgcagttgat gcgatgtccg acaatcacga
     1261 tcacaagttt ccgagatctg actgccaaag acaatcgggt ggatgcaacc agtgatccga
     1321 tacccgtttt tggctttgac taccatgccg acactctctc gcgagagacg gttaatgcgg
     1381 ttcgggtgca ggcgagaaac atttctggtt tcaccccgca gatggatttg cggccaattc
     1441 tgggcggtct ggacgggttg agcggtgtcg agctctatcg cgatggcaac ggtaacggtg
     1501 tctgggacac gacggatgtg cgagtcgcaa tcaaagccag tcagtgggag aacgtctact
     1561 acgctgacaa tccggggcct gatggtgtcc tggcgacgtc agacgatttg ggtaccttgg
     1621 gagtctacga cccgggtaca gacgagctgt tcctcgacga cggctttatc acgggatact
     1681 atgatctcgg ggacacgctc atcgaggacg gaacagacaa cattcgtcat acgctgccaa
     1741 ccagctatga tatccaggac gcgacgcttt acgcgctgcc ttcggggcag gagcgttggt
     1801 cgttgttgat ggttccacag acgccgattc cggtgccgca ggcgtttgat ggtatt
//
LOCUS       DAOQUC010000889         1856 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_81715_length_1856_cov_2.391449, whole genome shotgun sequence.
ACCESSION   DAOQUC010000889 DAOQUC010000000
VERSION     DAOQUC010000889.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1856
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81715_length_1856_cov_2.391449"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..473
                     /locus_tag="PKH07_13725"
     CDS             <1..473
                     /locus_tag="PKH07_13725"
                     /inference="COORDINATES: protein motif:HMM:NF014801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HNT36042.1"
                     /translation="TPHKDPEARGAFVGLTMRHQKPHLVRALLEGITFGLRDSLEIIR
                     AQGVDVKEICATGGGAKSAFWRQLQADVYGQPIVTTNSTEGPALGAAILAGVGVGAYE
                     SVKQACRTIIKVTDRVEPNNANAERYSEVYQEFTNLYPALKERFKSITRLVERS"
     gene            706..1590
                     /locus_tag="PKH07_13730"
     CDS             706..1590
                     /locus_tag="PKH07_13730"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HNT36043.1"
                     /translation="MNDRRTVVRPVAHASWWHCIVLMLVSLCLCGFTGLFRKDHVHIR
                     VMTYNIRVGVGGGTLRLQSEQGLKKVAHIVRKHKPDILLVQELDINAPRTQYVDQGQW
                     LMHHLGFPHAAFGSAIREGTGSYGVAIFSRFPDNIAATKYELFKPDYPNRPDYFSEQR
                     VLLKAAVNIKGTTVTFLCTHLGLTSDQRTQQVQNILRHVADVETPIILGGDFNSESIS
                     REMRPFKTRFYDVFDALRMDIKQRLTYPAGLTPGQAIDTIYATPDVKVISAKVILDKT
                     LASDHNPVIAVLRIPIGQ"
BASE COUNT          427 a          493 c          508 g          428 t
ORIGIN      
        1 gtacaccgca taaagacccc gaagcgcgag gcgcttttgt cggtctgacc atgcgccacc
       61 agaagcctca cctggttcgg gcactcttgg aaggcatcac tttcggactc agggattctc
      121 tggagatcat ccgcgcacag ggtgttgacg tgaaggagat ttgtgcgact gggggaggtg
      181 ccaagagcgc tttctggcga caacttcagg cggatgtcta cggacagccg atcgtcacca
      241 ccaactcaac ggaaggacct gctctgggcg cggcgattct ggcgggcgtc ggtgtcggtg
      301 cctatgaatc agtgaagcag gcttgtcgaa cgatcatcaa ggtcaccgat cgcgttgaac
      361 caaacaacgc aaatgcagaa cgctatagcg aagtctatca ggaattcaca aacctctatc
      421 cagcactcaa agagcgattc aagtcgatca cccggctggt agagcgttct tgatgtaacg
      481 ttgcgatagc agaagtgtcg ggtgcgtcga gacgatcgga cgcaccgtct cagagttgaa
      541 caagaagaca acggatgcgt ctagcgacct attctacgtc tctcgataaa tgacaggact
      601 cctctgaact tggctccatc gggagatatt cccgatttga ctttcttcca aaggatatca
      661 aaggtatatt cctcacagtc tcttctgttt gggaggttca ggctgatgaa tgatcgaaga
      721 accgttgtgc gcccagtggc tcacgcctct tggtggcatt gcattgtttt gatgcttgtg
      781 tcgttgtgtc tgtgcggatt cactggcctg ttccgaaaag accatgtgca tattcgcgtg
      841 atgacttaca acatccgtgt cggtgtggga gggggtacgc tgcgccttca gtccgagcaa
      901 ggcctgaaaa aggtggcgca catcgtccga aaacacaaac cggacatcct gctggttcag
      961 gaactggaca tcaatgcgcc aagaacgcaa tacgtggatc agggacaatg gctgatgcac
     1021 catctggggt tccctcacgc cgcattcggt tcggcgattc gggaaggcac cggcagctat
     1081 ggtgttgcga tcttcagccg ctttccagac aacatcgctg ccacaaaata cgaactcttc
     1141 aagcccgact atcccaaccg tcctgactat ttcagcgagc aacgcgtgct tctgaaagcg
     1201 gcggtgaata tcaagggcac gacggtcaca tttctctgca cccatctggg gctgacttcg
     1261 gatcaacgca cccaacaggt tcagaacatt ctgcgccacg ttgccgatgt tgagaccccg
     1321 atcatcttgg gaggtgattt caactcggaa tccatctccc gagagatgag gccgttcaag
     1381 acgaggttct acgacgtctt tgacgcactt cgaatggaca tcaagcagcg actgacctat
     1441 cccgcggggc tgacacccgg acaggccatt gatacgatct atgccacgcc agatgtcaag
     1501 gtgatttcgg ccaaagtgat cctggataag actctggcat ctgaccacaa tccagttatt
     1561 gccgtgctca gaattccgat cggtcagtag gcaacggctg caacgttccc actcccatca
     1621 accgagggcg ggatgacgag ccgagacaga ggttgtctga atgatggaga aacctctatg
     1681 gtattcccca gattgaatca acgcagacac ggtttcacct ggcgtttgtt ctgtatcctt
     1741 ttgttcgttt tggtctcgtg tgcttgttgc ggagtcccaa tttccaggcg ggggcagggc
     1801 gctattcccg atggcggggt cgtgtatctg gacgtgcagt cccaatcaac ggtttc
//
LOCUS       DAOQUC010000890         1850 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_82117_length_1850_cov_3.152646, whole genome shotgun sequence.
ACCESSION   DAOQUC010000890 DAOQUC010000000
VERSION     DAOQUC010000890.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1850
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82117_length_1850_cov_3.152646"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            150..653
                     /locus_tag="PKH07_13735"
     CDS             150..653
                     /locus_tag="PKH07_13735"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNT36044.1"
                     /translation="MDISKLKEGLFDKILFCTDFSKNADLAFDYAIYAARRHPGSTLY
                     LLHVIPEPEAQFWKTYLYEVDGIDEKARQDIDARIAEVYRPRVPEEVHLEVVIRIGRD
                     YVKILEFAEEANVDLIVIGRQGRSSWGRVLFGSVMEKVTRKAQCAVMLIPLSAASEHA
                     DSSPDSV"
     gene            683..>1850
                     /locus_tag="PKH07_13740"
     CDS             683..>1850
                     /locus_tag="PKH07_13740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36045.1"
                     /translation="MECSTPNIEDYATDDKRVVRRGNITRTLSDLLSSSQWLPCFLGN
                     GYAGACFDPFGSMAQPSSDMRPKVVGQLLLHNLYHHVHGNFGMDFAAPIGRLIIDHWA
                     QQHKALSVASICDYHQELDIFDGVLETHIACNDGHEFEQEQFFSQMHKNLFSARIRNT
                     GSKSHKVTLRFEPILAYDLDFEQTLRAEATLQCTDESLRWRVDTGSVTTWLEFWMQVS
                     PNASVRQLEQSFATVIEPGGECTIWMIVMSDLEVTNPIEKIQEIFDFVFEQGFDAVSQ
                     AHKSWWTDFWSESLLALPECALDLQKIWLRGNYYLGQNFLDSKIPHPPPAFGFAATAT
                     PSFSPEGVLLSYQNLLTANHLRHASFNAAFWFDISPNAQNYCQQILRRTGAAYPW"
BASE COUNT          432 a          474 c          447 g          497 t
ORIGIN      
        1 tcccctttct gtacatgttg atcgtggcat cagtgatctt gctggcattt ccacagctgt
       61 gcctgtttct gcccaagttg gtcagatatg ttccaaggta gcttcgagtt ttcaccttcg
      121 gcatcgcgaa cagacgcaag gaggggacta tggatatcag caaactcaaa gaggggcttt
      181 ttgacaagat tctcttttgc accgatttct caaagaatgc cgatcttgct tttgactatg
      241 ccatctatgc tgcacggcgt caccctggct caacgctcta cctgcttcac gtcatccctg
      301 aaccggaagc tcaattctgg aaaacttatc tctatgaagt tgacggcatt gatgagaaag
      361 ctcgccagga tattgatgcc cgtatcgcag aagtttatcg gcctcgtgtt cctgaagagg
      421 tccaccttga agtggtgatc cggatcgggc gggactacgt caagatactt gaatttgcag
      481 aagaagccaa tgtcgatctc attgtcattg gtcggcaagg gcgcagttcc tggggacgtg
      541 tgctctttgg aagtgttatg gaaaaggtga ctcgaaaggc ccaatgcgcc gtcatgttga
      601 ttcctttgtc cgcagcatcg gaacatgcgg attcttcacc cgattctgtc tgaaccattt
      661 cagattccag gaattccggc agatggaatg ctcgacacca aacatcgaag actatgcgac
      721 tgatgacaag agagtcgtca gaaggggcaa cataactcgt actctctctg atcttctctc
      781 ttcctcacaa tggcttccgt gttttctagg aaatggatac gctggcgcat gctttgaccc
      841 attcggttcc atggcgcaac cctcttcgga catgcgtcca aaggtggtcg gacagctttt
      901 gcttcataat ctctatcacc atgttcatgg caatttcgga atggattttg ctgcgccgat
      961 tggcaggctg atcatcgatc attgggcaca acagcacaag gcactgagtg tcgcttccat
     1021 ctgcgattat caccaggaac tggatatctt cgatggtgtc ctagaaacgc acattgcctg
     1081 caatgacggt catgagtttg agcaggaaca gttcttctcc cagatgcaca agaacctctt
     1141 ctctgcgcgg atcagaaaca cgggcagcaa gtcccacaag gttactcttc gcttcgagcc
     1201 aatacttgca tatgacttgg atttcgaaca gacgctacgt gctgaagcaa cacttcaatg
     1261 tactgatgaa tcccttcgct ggagagtaga taccggaagc gtgacaactt ggctggaatt
     1321 ctggatgcag gtgtctccta acgcctcagt tcggcagttg gaacagtcct tcgccacggt
     1381 tattgagcca ggtggggaat gcactatctg gatgattgtg atgtctgacc tcgaagttac
     1441 gaatcctatc gagaagattc aggagatctt cgatttcgtc ttcgagcaag ggtttgatgc
     1501 cgtttctcaa gcacacaagt cgtggtggac agacttctgg agcgaaagcc tgcttgcact
     1561 tccagagtgt gcacttgatc tccagaaaat ctggttgcgc ggtaattact acctcgggca
     1621 gaatttcctg gattcgaaga ttccccatcc gcctccagcc tttggttttg ccgcaacagc
     1681 aacaccttct ttctctcccg aaggcgttct gctgagttac caaaatcttc tcactgcgaa
     1741 ccatctcaga cacgcctctt tcaacgcggc tttttggttt gatatttccc caaatgcaca
     1801 gaactactgc caacagatac tgcgacgcac gggggctgcc tatccctgga
//
LOCUS       DAOQUC010000891         1849 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_82188_length_1849_cov_3.282609, whole genome shotgun sequence.
ACCESSION   DAOQUC010000891 DAOQUC010000000
VERSION     DAOQUC010000891.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1849
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82188_length_1849_cov_3.282609"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..873)
                     /locus_tag="PKH07_13745"
     CDS             complement(<1..873)
                     /locus_tag="PKH07_13745"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNT36046.1"
                     /translation="MMECAIQTRNLARRYGKKTVINDLELSVPTGSVYGLFGRNGVGK
                     TTTIRMLMGLIRRHGGEVSVLGLDPERDGVKVKSRVGYVPESPEFYEWLSVEKTIGLV
                     ANYQKNWDDSLAERLVKDYGLDLKAKVKALSKGTRAKLALLLALAHRPEVLILDEPTG
                     GLDPAMRREFFESVLRALPEQGCTVFISSHLVNEIAPLVDQVGFLREGKLLLTDETEN
                     LLEKFKRIRAVFALGQVPERISLAGLLRVERTEREAVITVSEFDEARMLAEIAQLSPT
                     NVTVESLTLEDIFIE"
     gene            complement(870..1259)
                     /locus_tag="PKH07_13750"
     CDS             complement(870..1259)
                     /locus_tag="PKH07_13750"
                     /inference="COORDINATES: protein motif:HMM:NF012610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HNT36047.1"
                     /translation="MLFSLDPKSPKSIYRQIVDRVKSDVARGALDAGDQLPTIREAAA
                     QLRVNRNTVARAYRELEQQGIIYTRPGGGSFVADAVSDLQKRERVRILEEMAAEMVVQ
                     AFHFQIAPDELIEIITKQIETMERSRK"
BASE COUNT          404 a          546 c          451 g          448 t
ORIGIN      
        1 ctcgatgaaa atgtcctcca atgtcagcga ctccacagtg acgtttgttg gtgaaagctg
       61 cgctatctcg gcaagcatcc ttgcctcgtc aaattcactg acggtgatga ccgcttcgcg
      121 ctccgtgcgt tcgactcgca gcaagccggc aagggagatc cgttcgggaa cctgccccaa
      181 cgcaaagacc gcgcgaatcc tcttgaactt ctccagcagg ttctccgttt catcggtcag
      241 gagcagtttc ccttctcgca ggaaaccgac ctgatccacc aatggagcga tctcgttcac
      301 cagatgtgac gagatgaaga ctgtacaacc ttgttcagga agtgctctca ggacactttc
      361 gaaaaactct cttctcatcg caggatccaa accgccggtc ggttcatcca gaatcaacac
      421 ttcaggtcta tgcgccaaag ccagaagcag cgccagtttc gcccgagttc ctttggaaag
      481 cgccttgact ttggctttca gatccagtcc atagtccttg accaaacgct ccgccagcga
      541 gtcatcccaa ttcttctgat agttggcgac cagtccgatg gtcttctcca cagagagcca
      601 ttcgtagaat tccggactct cgggcacgta gccaacacgc gacttgactt tcaccccatc
      661 gcgttccgga tccaagccca gcacagagac ctcaccccca tggcgtcgaa tcaaccccat
      721 cagcatccga atggtggtcg tcttgcccac gccgtttctg ccgaacagtc catagacgct
      781 tccggtcgga accgacagtt ccaaatcatt gatcacggtc ttctttccat atcgtcgagc
      841 caaatttcgg gtttgaattg cacattccat cattttcgac tccgttccat ggtttcgatt
      901 tgtttcgtga tgatctcaat gagttcgtcg ggcgcaattt gaaaatggaa ggcttgaacc
      961 accatctcag ccgccatctc ttccagaatc cgaacacgct cccgtttctg aaggtcggac
     1021 actgcgtccg caacaaacga tccacctccc gggcgcgtgt agatgattcc ctgctgctcg
     1081 agttcccgat aggcacgcgc cacggtgttg cggttgacgc gcaactgggc tgctgcctct
     1141 cggatcgttg gaagttgatc ccctgcgtcc aaagcgcccc gggcaacgtc tgacttgact
     1201 cgatccacta tctgccgata gatgctcttg ggcgattttg gatccaacga gaagagcatg
     1261 agacggttca cctcaaccta tttgtcatag ggagtatatg acaagtaaga caaaagtcaa
     1321 ggggagattt ccattcaccg tactggctta gttatgccgg tgattcagcc aggatatgct
     1381 ctcccgaaaa tcccccctgc cctccttacc gaagggtaag aaggctcttg ccccccttaa
     1441 caagggggga tcaagggggg atttctcatc cccttcaggt gcgccgtagg cccatgagga
     1501 gtttcggaag caaagcggag tcttcggagc aacgctgccg aagtccgctg tctggctgtt
     1561 cccgcttcgg cagcgtcgca gagcctctgc taccgaagca tgcctccacc ccctccgtaa
     1621 ctgagccagt accaccgtgg tcagacttgt gttgcattct ggcacacatt tatccatgat
     1681 tctcccgggc aggctgctca ggataaaggt ggcaactgtt gaacggagat cgaatctata
     1741 ccattcttgt atgcgtcacg gcaggaatgt gcctgttcct gattgtctac aatcgtcttt
     1801 tgaatgtgcg tgatggtgct ggaaaggtct atgcgatccg acttgtgtt
//
LOCUS       DAOQUC010000892         1848 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_82276_length_1848_cov_3.183491, whole genome shotgun sequence.
ACCESSION   DAOQUC010000892 DAOQUC010000000
VERSION     DAOQUC010000892.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1848)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1848)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1848
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82276_length_1848_cov_3.183491"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..842)
                     /locus_tag="PKH07_13755"
     CDS             complement(<1..842)
                     /locus_tag="PKH07_13755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36048.1"
                     /translation="MYCAQVVQGEGFTIFLRKPVDDQIYLTVQTGGIPQRLDHLVTSI
                     YQSIDDILQDAKACVVHERIFGSNTCQHSILESRNRSLSKCCGDTGSGLFTFIEGKPV
                     WGEGLAGIQVLAVSPRTSTEKVQVIYEKGAAVGRIWRRHGNTFVMLQNLHGQLSNSPA
                     EDSRTAEVGRMFERTERLLKQVHGDYRDVVRTWLYLSEILKWYPQFNEVRNSAYARFG
                     IMPSTTDHEPVHRLTLPASTGIQAENPLGAACLMDVLAIVGQSRPPVVQMSNSRQKDA
                     FRYRS"
     gene            complement(852..>1848)
                     /locus_tag="PKH07_13760"
     CDS             complement(852..>1848)
                     /locus_tag="PKH07_13760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36049.1"
                     /translation="DRFLFQVRSTEYGRPMVMVYDGEVKAVDSRTDANPLTAVYCSWH
                     PNGKIVAMSGNRITQFFHTAGQEGREVFDHESDLALLNVETNTTTTTQDIMLPDRLET
                     YPNWSADGRFLYFCSGPQLPVERYKEIKYDLMRIAYDVDSDSWGAMETIVSASDTGLC
                     VTHPKPSPDGRYVLFCMCDHGNFSIIQSSSDLYLLDTSTGAYRRLDINSDEVESYHSW
                     SSNSRWFVFSSKRRDGLLAKPYFCHVDEKGRVSKPFLLPQRDPLFYDSFLKTFNVPEL
                     INGPVRVSSKEISRTVWNKADHLKAKLDPKVALPKRDEKKEPSKDIPWRPASAGR"
BASE COUNT          465 a          496 c          441 g          446 t
ORIGIN      
        1 gaacgataac gaaacgcatc tttctgtctt gaattcgaca tctgaacaac tggaggcctg
       61 ctttgaccca cgatagctag aacatccatc aagcaagcag ctcccaaggg attctctgcc
      121 tgaatccctg tgctggcggg aagcgtcaag cgatgaactg gctcgtgatc tgtagtagaa
      181 ggcattatgc caaatcgggc atacgcactg ttgcgaactt cgttgaactg cggataccat
      241 ttgagaatct ccgacagata cagccatgtc ctgacaacgt cgcggtagtc tccgtgaacc
      301 tgcttgagca gtctctcggt acgctcaaac atccgcccca cttctgccgt gcgggagtcc
      361 tctgcaggtg agttcgacaa ttggccgtgc aaattctgga gcatcacaaa agtattgccg
      421 tggcgacgcc atatccgacc aacagcagca cccttctcgt agatgacctg aaccttttca
      481 gtactagtcc ttggagagac agccagaacc tgaattccgg caagcccttc tccccagact
      541 ggcttgccct ctatgaaggt gaacagccca ctccctgtat cgccgcaaca cttggaaaga
      601 gagcgattcc ttgattcgag gatggaatgc tggcacgtat tgctgccaaa tatcctctca
      661 tggacaacgc atgctttggc atcttgaagg atgtcgtcaa tactttgata gattgacgta
      721 acaagatgat cgagtcgctg gggaatgcca cccgtctgca cagtcaagta tatctggtcg
      781 tccacgggct tgcgtagaaa aatcgtaaac ccttcccctt gaacaacttg agcacaatac
      841 atctgcaatt cctatcttcc tgcactcgct ggacgccaag gtatatcctt ggacggctcc
      901 tttttctcat ccctctttgg caatgcaact tttggatcta acttagcctt gagatgatct
      961 gccttgttcc agaccgtccg actgatctcc ttgctactga cgcgcacagg tccgttgatc
     1021 aactcaggca cgttaaatgt cttgagaaag gaatcataga acaatggatc tctctgcggc
     1081 aacagaaaag gcttcgaaac tctgcccttc tcgtccacat gacagaaata cggcttggcg
     1141 agaagtccat cccgtcgttt gctgctgaaa acaaaccagc ggctattgct cgaccacgaa
     1201 tgataggact caacctcatc ggagttgata tccaggcgac ggtatgctcc tgtcgaagtg
     1261 tccagaagat agagatcact cgatgactga atgatcgaga agttcccatg atcgcacata
     1321 cagaacaaaa cgtatcgccc gtcaggggaa ggcttgggat gcgtcacaca caggccagta
     1381 tctgaggcgg aaacgatagt ctccatcgct ccccaggaat cgctgtctac atcgtacgcg
     1441 atccgcatca agtcgtactt gatctctttg taccgttcaa cagggagctg agggccactg
     1501 cagaaataga ggaaccgacc atcagcagac cagtttgggt aagtttccag acgatcaggc
     1561 agcatgatat cttgtgtcgt ggttgtcgtg ttggtctcaa cgttgagaag agcaaggtct
     1621 gattcgtggt cgaacacttc ccttccttcc tgacctgccg tgtggaagaa ttgtgtaatc
     1681 cggttgccgg acatggcaac gattttccca ttgggatgcc aagagcagta aacagcagtc
     1741 agcggatttg cgtctgttcg cgagtcaaca gcctttacct ctccatcata aaccatgacc
     1801 atgggtcgtc cgtattcagt gctcctgacc tgaaacagga aacgatca
//
LOCUS       DAOQUC010000893         1847 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_82323_length_1847_cov_4.199777, whole genome shotgun sequence.
ACCESSION   DAOQUC010000893 DAOQUC010000000
VERSION     DAOQUC010000893.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1847)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82323_length_1847_cov_4.199777"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..855)
                     /locus_tag="PKH07_13765"
     CDS             complement(<1..855)
                     /locus_tag="PKH07_13765"
                     /inference="COORDINATES: protein motif:HMM:NF015604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046559 - alpha-glucuronidase activity
                     [Evidence IEA];
                     GO_process: GO:0045493 - xylan catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucuronidase family glycosyl hydrolase"
                     /protein_id="HNT36050.1"
                     /translation="MPTLTVCRNLHSNWSILNASSDSEVCRFAAEELQRYLKLLSGCL
                     LPIQDKLREEDVFLVGLRRDIPATDRQNIPVPAEGFDGYAISINERSICIAGDNERGV
                     LYGVYDLLERLGCRWFYPMQDPKDPEVVPKLDAVSFETGTWSIASPIPIRLCNASSFF
                     FEIVPEIMRTQLDVAMKARYNGMAWQCDHRSPVGDQYREMERAGIIDEIKKRGMLFHG
                     PAHSFQHFLRDDDYFESHPEWFGMRDGKRVHQVYGGSQFCWSNSQARRQFVLNAERFV
                     LDCPALDIF"
     gene            complement(1032..>1847)
                     /locus_tag="PKH07_13770"
     CDS             complement(1032..>1847)
                     /locus_tag="PKH07_13770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36051.1"
                     /translation="RLDGEGRPIISDWSEDHSGVWIATPDCNTPFVALLTEENRNSYG
                     LCLNHGSIMAVWAEGSGTGRVIYTIDEDYTVGGETAGSVLRYDIGDATQYADAPTVHY
                     NDTANGNLVISTRSQHVRDGSGWWITQSLAEQSASTPWLFRWNGSTVDYNSASAGLSI
                     NRSYGALDIDLARRRLVLGGLGTVSLLNIASAPSSVLVVGEIDAGGDHVQDVSFDAAG
                     NFLQINSSVRRLRAYSPNDGANSFVTPCRTSQQLIVGKVGSTGISQEIWNPYR"
BASE COUNT          448 a          512 c          496 g          391 t
ORIGIN      
        1 aaagatgtcc agagccgggc agtccagcac aaagcgttcc gcattcaaga cgaactgtct
       61 tcgcgcctgg gaatttgacc aacagaactg ggagccgccg tagacctggt gtacccgctt
      121 gccatcacgc atcccaaacc attccggatg ggactcgaag taatcatcat ctcggagaaa
      181 gtgctgaaaa ctgtgggcgg gaccatgaaa gagcatcccg cgcttcttga tctcgtcaat
      241 gatgcctgca cgctccatct cgcggtactg atcgccgaca ggggaacgat gatcgcactg
      301 ccatgccatg ccgttgtagc gcgctttcat cgccacatcc agttgtgtgc gcatgatctc
      361 aggcacgatc tcgaaaaaga aagaggaagc gttgcacaag cgaatcggaa tcggtgaagc
      421 gatactccag gttcccgtct caaaagacac agcgtccagc ttgggcacca cttcgggatc
      481 tttcggatcc tgcatcggat agaaccaacg gcagcccaat cgctccagga gatcatagac
      541 tccatagagc actcctcgtt cattgtcgcc cgcgatgcaa atgcttcgtt cattgatgga
      601 aatcgcgtac ccatcaaagc cttccgctgg aacaggtatg ttctgccgat ctgtcgcggg
      661 aatatctcgt cgaagcccga caaggaacac atcctcttcg cgcagcttgt cctgaattgg
      721 gagcaggcaa ccgctgagaa gtttcaaata acgctgaagc tcttcggcgg caaaccgaca
      781 gacttcactg tcgctggacg cattgagaat gctccaattg gaatgcagat ttcggcagac
      841 ggtgagagtg ggcatagggt ctcctcctga gttcgtgcaa tggtgggaat tgatagcagt
      901 ttcgaaagca gtccgcaacc gttgagggat tcgcgattga ccttgtacgg tgccaagtgt
      961 gattctgaat ggagcaaagc ggcgggaaga atctcaaacg ctcaaaacta ccagccagtt
     1021 gcttcactga cctatctgta cggattccaa atctcctgtg agatgccagt ggaacctacc
     1081 tttccaacga tgagttgctg actggttcga cagggagtca caaaactatt ggctccgtca
     1141 ttgggggaat aggcacgcag ccgacgaacc gagctgttga tctgaagaaa gttgcccgct
     1201 gcgtcaaaag agacatcctg gacatgatcg ccccctgcat caatctcgcc caccacaagt
     1261 acagacgacg gtgctgaggc tatgttcagc agagagactg ttcccaatcc acccaagaca
     1321 agacgccgtc gtgcgagatc aatgtccagg gctccgtagc tgcggttgat ggatagtcca
     1381 gcagaagcgg agttgtaatc caccgttgaa ccattccacc gaaacaacca tggcgtggac
     1441 gcgctctgtt ccgcaagaga ctgagtgatc caccagccgg aaccgtctcg gacatgttga
     1501 ctgcgcgtgc tgatgacaag attgccgttc gcggtgtcat tgtagtgaac cgttggcgca
     1561 tcggcatact gcgttgcatc gccaatgtca tagcgcagaa cgctgccggc agtctccccg
     1621 ccgacggtgt agtcctcatc aatggtgtag atcacacggc ctgtgccgga gccttccgcc
     1681 cagacagcca tgatgcttcc atggttgaga caaaggccgt atgagttccg attttcctcc
     1741 gtcaaaagcg caacaaaagg ggtattgcag tccggtgtgg cgatccagac tcccgagtga
     1801 tcctccgacc agtcggagat aattgggcgg ccttccccgt cgagtcg
//
LOCUS       DAOQUC010000894         1847 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_82351_length_1847_cov_2.468750, whole genome shotgun sequence.
ACCESSION   DAOQUC010000894 DAOQUC010000000
VERSION     DAOQUC010000894.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1847)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82351_length_1847_cov_2.468750"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1847
                     /locus_tag="PKH07_13775"
     CDS             <1..>1847
                     /locus_tag="PKH07_13775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36052.1"
                     /translation="WNGNNIEVVLVNGEPVPALDTGTEAGNDYFIVVRTSDSIQFGDR
                     FRMGVRQNGVILSGLAMGNVTVSGTLASMVPTLVASLTEQPTHALRIPGENVAVFGLT
                     IADNGFDQSLQEMKLRFDEGEGFIETVQSQSFLNREALLGLGPVPVYDPEIEERYDPS
                     IGMVTSTVIIPVTSVVQVQAPVSFTLSRPPLLTLNMSHSRPEDVEIWLFPPGSQGALC
                     FATLGTASCTDHYRYPPNYGGILIYDTVFDPRNPLPANINLANFPIPPAMLPQQSNAQ
                     GDWYVLVKDVVRNGQRGTLNNWSLTFEWSTRTFGSFSVSDIMSAADNGIALWFDANAN
                     GFFDGAPLDQQLALQGDPRIGDAGPNTVNLKLVTPFAVPDPQDFVPDLFVVIRPSETM
                     GKGDTFTVTIPPRGITYDGGYGSLMPSSSWESGPLQGNLNVRPTITMIDPPVGNARAN
                     RYYDIRWIDDDPDDNAAICLYYDNDRSGFDGVKINVPPSPPVLDEDPDGYTNPMGPGD
                     AFRWDTSRVPPGNYYIYAVITDGFSTPYQVYSKGYVTVSNPVRVHLSDLSEQNARVDA
                     TCPPTAVLGINAYDQGIYSTLDGVTVELHDLSGTVTPDDFLPFSPGSSGG"
BASE COUNT          407 a          490 c          489 g          461 t
ORIGIN      
        1 tggaatggca acaatatcga agtggttctc gtcaatggtg aacctgttcc cgctttggat
       61 acgggcaccg aagcaggcaa cgactacttc atcgttgtgc gcaccagcga ctcgatacag
      121 ttcggagatc gtttccgaat gggtgtcagg cagaatggtg tcatcctttc cggcctggcc
      181 atgggcaatg ttacagtgtc tggtactctg gcatccatgg ttccgaccct cgtcgcgagc
      241 ctgactgagc agccgaccca tgcactcaga atccctggag agaatgtcgc tgtctttggt
      301 ttgactattg cagacaatgg ctttgaccag tctctgcagg agatgaaact taggtttgat
      361 gagggggaag gtttcattga gactgttcag agccagtcgt ttctgaatag agaagcactg
      421 ctgggtctcg gccccgttcc ggtatacgat ccggagattg aagagagata cgatccgagc
      481 attgggatgg ttacttcaac agtgataatc ccagttacat cggttgtaca agtccaggca
      541 cctgtcagct ttactctttc tagaccgcca ttgttgactc tgaatatgtc tcattctcgt
      601 cccgaggatg ttgagatatg gctctttccc cctggatcac aaggggcttt gtgcttcgcg
      661 actcttggaa ctgcatcttg taccgatcac taccgctatc cgccaaacta tggcggcatt
      721 ctcatctatg atacggtctt tgacccgagg aacccgctgc ccgccaacat caacttggcg
      781 aactttccca taccgccagc tatgttgcca caacagtcga atgctcaggg cgattggtat
      841 gtacttgtca aagatgtggt tcgcaatggg cagcgtggaa ctctgaacaa ctggagtctt
      901 acatttgaat ggtctacacg aacatttggc tcattttccg tttcggacat catgtccgcg
      961 gcagataatg ggattgctct gtggtttgat gcaaacgcca acggtttctt tgatggtgcg
     1021 cccttggatc agcagttggc actgcaggga gatccccgca tcggcgatgc gggacccaac
     1081 acagtcaatc tgaaactggt cacgccattt gctgttccag acccccagga ctttgtcccg
     1141 gatctgtttg ttgtcatcag acccagcgag acgatgggca agggtgatac cttcacggtg
     1201 acgattcctc cgcgtggcat cacctacgat ggcggctacg gttccctgat gcccagctca
     1261 agttgggaat cgggcccatt gcagggaaat ctgaatgttc gtccgacgat cacaatgata
     1321 gatcctcctg tcggcaacgc aagggcgaat cgttactatg acattcgttg gatagacgat
     1381 gatcccgatg acaatgcggc aatctgtctg tattacgata acgaccgatc aggctttgat
     1441 ggagtcaaga tcaacgtgcc acccagtcct ccggtgctcg acgaagatcc tgacggatat
     1501 accaatccga tgggtccggg agatgccttc cgatgggata cctctcgggt tcctcccggc
     1561 aactactata tctatgccgt gatcacggat ggattctcga cgccgtacca ggtctacagc
     1621 aaaggctatg ttacggtgag caatcccgtc cgagttcact tgtccgatct gagcgagcag
     1681 aacgcccgag tggacgccac ctgtccaccg acggcagtgc tcgggattaa cgcatacgat
     1741 caggggatct actctacgct cgatggtgtt accgttgaac tgcacgatct gtccggcaca
     1801 gtcactccgg acgacttcct gcccttcagt cctgggagca gtggagg
//
LOCUS       DAOQUC010000895         1846 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_82438_length_1846_cov_2.127862, whole genome shotgun sequence.
ACCESSION   DAOQUC010000895 DAOQUC010000000
VERSION     DAOQUC010000895.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1846)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1846)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1846
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82438_length_1846_cov_2.127862"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1398
                     /gene="priA"
                     /locus_tag="PKH07_13780"
     CDS             <1..1398
                     /gene="priA"
                     /locus_tag="PKH07_13780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HNT36053.1"
                     /translation="VGILHSGLSSGERFDEWSAAREGRVRVIVGTRSAVFCPLDNIGL
                     IVVDEEHDPSYKQDDPAPRYHARDVAAYRAAAVGAVAVFGSATPSLESYWNSRTGKYD
                     LLTLPERAVQHGLPELSLIDMRARAENESVVSQELVDACRACLDRSEQAILFLNRRGF
                     ATNLLCRHCGETITCEKCSVALVYHQDQRRLRCHHCGFSRPEPTRCPSCSQEWLKYKG
                     VGTEQVVEVMEQLLPGVRVGRMDTDTVQTRTAYNDLFSALARGEMDILVGTQMIAKGL
                     DFGGVSLVGVICADMALFFEDFRAAERTFALLTQVAGRAGRGRTAGKVLIQTYCPQHY
                     SVRAALDQNYQAFFDQEIRYRKLLSLPPYRRLVNIRISSGEEAIAKATAFELSNAIRL
                     AFDELGIRTVRIIGPGECPIYRVRNQFRWQLAIAGRSQTDNARVLTHAAVQKHIRSAK
                     SKLKITVDVDPQSFL"
     gene            1395..>1846
                     /locus_tag="PKH07_13785"
     CDS             1395..>1846
                     /locus_tag="PKH07_13785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36054.1"
                     /translation="MTQFAGGLPRLPIALFSPDAVDSDHNYAAPWLNLGAHIREAYQM
                     ENTIQGFGQEHGNTSTGTISVIPVVRSAAAVLGILTMLIGLFYATRIFSLIYGVLKDP
                     DGFQTTLGKWITVLGGDQLDIVIAGSAFPLANILALGVLGGGAIALVW"
BASE COUNT          380 a          495 c          538 g          433 t
ORIGIN      
        1 gtcggcattc ttcacagcgg cctttcctcg ggagagcgct ttgacgaatg gagtgctgcg
       61 cgtgaagggc gtgtgcgcgt cattgtcgga acacgctcgg ccgtcttctg tcccttggac
      121 aacatcggac tgatcgtggt ggatgaagaa catgatccgt cctacaaaca ggacgatccg
      181 gctccccgct accatgcgcg ggacgtcgca gcatatcgtg ccgcggcggt tggagccgtg
      241 gcagttttcg gcagtgctac cccatctttg gagtcctact ggaactccag gacgggcaag
      301 tacgatcttc tcacactgcc agagcgtgcc gtgcagcatg gtctgcccga actgagtttg
      361 attgacatgc gggcacgtgc agaaaacgaa tctgtggtgt cacaggagtt ggtggatgcc
      421 tgccgggcat gccttgatcg atcggagcag gccattcttt ttttgaatcg aagaggcttt
      481 gccaccaatc tgctctgccg ccactgtgga gaaaccatca cctgtgagaa gtgttccgtg
      541 gctctggtgt atcaccagga tcagcggcgc ttgaggtgcc accattgtgg cttcagccgt
      601 cccgagccaa cccgatgtcc cagttgttct caggagtggc tcaagtacaa gggtgtcgga
      661 accgaacagg ttgtggaagt gatggagcaa cttcttcccg gtgtgcgcgt gggtcggatg
      721 gacaccgaca cggttcaaac ccgaaccgct tacaacgatc tcttttcagc ccttgcgcgg
      781 ggtgagatgg acatccttgt cggaacgcag atgatcgcca agggattgga ctttggcggc
      841 gtgtcactcg ttggagtcat ctgtgcagac atggctcttt tttttgagga ttttcgggct
      901 gccgagcgaa cctttgcact gctgacgcag gtggctggca gagcgggacg gggacgtacc
      961 gcaggcaagg tgctgattca aacctactgc ccgcagcatt acagcgtccg cgccgcgctg
     1021 gatcaaaact accaggcgtt cttcgaccag gagattcgct accggaagct gttgagcctt
     1081 cctccctatc gtcggctggt caatatccgg atcagcagcg gagaagaagc catcgcgaaa
     1141 gccacggcat tcgaactcag caacgcgatc cgacttgctt ttgacgaact cggaatccgg
     1201 actgtcagga tcatcggtcc cggcgaatgc ccgatttatc gtgttcggaa ccaattccgc
     1261 tggcagttgg caattgcggg acgaagccag accgacaacg cacgagtttt gacacatgct
     1321 gcggttcaga agcacattcg tagcgccaag tccaaactga agatcactgt ggatgttgat
     1381 ccgcagtcgt ttctgtgact caatttgcag gcgggcttcc aaggcttcct attgcactgt
     1441 tttcgccgga tgcggtagac tcagaccaca actatgcagc gccctggttg aatctcggtg
     1501 cgcatatcag agaggcatat cagatggaga acacgataca gggttttgga caagaacacg
     1561 ggaacacgtc aaccggaacc atctccgtga ttccagttgt tcgttctgcg gctgctgtat
     1621 tgggcattct taccatgctc atcggtctat tctacgccac tcggatattc tcgctcatct
     1681 acggcgtatt gaaagatcca gatggatttc agaccaccct cggcaagtgg atcaccgtgc
     1741 ttgggggtga ccaattggac attgtcattg cgggcagcgc attccccctg gcaaacattc
     1801 tggctcttgg ggtgctgggt ggtggagcca ttgctcttgt gtggat
//
LOCUS       DAOQUC010000896         1845 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_82473_length_1845_cov_3.300559, whole genome shotgun sequence.
ACCESSION   DAOQUC010000896 DAOQUC010000000
VERSION     DAOQUC010000896.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1845)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1845)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1845
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82473_length_1845_cov_3.300559"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1298
                     /locus_tag="PKH07_13790"
     CDS             <1..1298
                     /locus_tag="PKH07_13790"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: protein motif:HMM:NF013071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HNT36055.1"
                     /translation="LYWMQASQRAEGNHALEYAIRRANELQRPLIVGFGLTSAFPEGN
                     CRHYTFMLEGLQLMAKGLQKRGIPLVVRIGSPPKVALELAQRASLVVTDRGYLRIERA
                     WRDEFAQYVPCHAVQIESNVVVPIALASSKQEIGARTLRPKIKRVLFDHLIPIKKEGL
                     KHRSIHLQIDSEPMARVEMLLAKLGLDASVKPSSSIHGGTDRARILLRKFIKTKLRLY
                     AESRSDPSLGIVSNMSPYLHFGQISPIEIALTIIDADGVPSSAKDAYLEELIVRRELA
                     INYCFFNSQYDQYEGLPAWAQRTLSEHAADPRPYRYRPEQLEQAQTHDPYWNASQREM
                     LITGTMHNYMRMYWGKKILEWTKRPQDAFALALYLNNKYQLDGRDPNSFAGVGWCFGT
                     HDRAWPERPIFGKIRYMNDAGLKRKFNIDDYVRQISHLE"
     gene            1584..>1845
                     /locus_tag="PKH07_13795"
     CDS             1584..>1845
                     /locus_tag="PKH07_13795"
                     /inference="COORDINATES: protein motif:HMM:NF016430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HNT36056.1"
                     /translation="MRDPDDADDAFQEALAKIWKSLPKILRHPNPQALILRICLNAAY
                     DLIRKRTRLRQREEEDSIVEDFPDNSASAFERLLGQERERQIM"
BASE COUNT          464 a          497 c          476 g          408 t
ORIGIN      
        1 ttctctattg gatgcaggct tcccaacgtg ccgaaggcaa ccatgcgctt gaatacgcaa
       61 ttcggcgggc aaatgaactc caacgacccc tcatcgttgg attcgggctc acctctgctt
      121 tccccgaagg caactgccga cactacacat tcatgttgga aggtctccaa ctcatggcca
      181 aaggtcttca aaagcgaggc attcctctcg tcgtgcggat cggctcaccg ccaaaggttg
      241 cattggaatt ggcgcaacgc gcttcgttgg tagtcacaga tcggggctat cttcgtattg
      301 agcgagcctg gcgggacgag ttcgctcaat atgtcccttg tcatgcagtt cagattgaat
      361 cgaatgttgt cgttccaatt gcactcgcaa gcagcaaaca agagattgga gccagaactc
      421 ttcgacccaa gatcaagaga gtcttgttcg accatctcat ccccatcaag aaagaaggac
      481 tcaagcatcg ttcgatccat ctgcagatag attccgaacc gatggcacgt gttgagatgc
      541 ttctggcaaa gcttggtctg gatgcatctg taaaaccgtc atcatcaatc cacggaggaa
      601 ccgatcgagc tcgaatcctg ttgcggaagt tcatcaagac caagttgcga ctgtacgctg
      661 agagcaggag cgatccgagc cttggcatcg tctccaacat gagtccctat cttcattttg
      721 gtcagatctc gcccatcgaa attgcactga cgatcattga cgcagacggg gttccctcgt
      781 cagccaaaga tgcatatctc gaggaactca tcgttcgccg tgaactggcg ataaattatt
      841 gctttttcaa ctcgcaatac gatcagtatg aaggactccc cgcctgggca cagcgaacgc
      901 tgtccgaaca tgccgccgat ccgcgcccat accgctatcg cccggaacag ttggagcagg
      961 ctcaaaccca tgatccctac tggaatgcat cccagagaga gatgctgatc acaggcacga
     1021 tgcacaacta catgcgtatg tactggggga agaaaattct tgagtggaca aaacgacctc
     1081 aggacgcttt tgcactggcg ctctatctca acaataagta ccaactcgat ggacgggatc
     1141 ccaattcctt cgccggtgtt ggctggtgct ttggcacgca cgatcgggca tggcctgaac
     1201 gaccgatatt cggcaagatt cgttatatga acgatgccgg tctgaagcgg aaattcaaca
     1261 ttgacgacta tgttcggcag atttctcatc ttgagtaggc catcgcctgc tggctcccac
     1321 tcttcagaag cctgtaacct ggagttttgt gacaacagga tctcccctct ggggcagcct
     1381 gctgaatctc aggctgtgca cctgacagat cgctgtggac aaaagaaccc gtgctgacgt
     1441 ctatagtagt gaggacacgg atgtccgcgg tcaacctgtg caggatatga aacgtcgaga
     1501 ggaggacgcc cttgattcaa agcgactacg agcgtttgat cgagcctatc gaagaccgaa
     1561 tgatcggatc catctggagg atagtgcgcg atccggacga cgcggacgac gcctttcaag
     1621 aagccctcgc gaagatttgg aaaagccttc cgaaaatact caggcacccc aatccgcagg
     1681 cgctgatctt gagaatctgc ttgaacgccg cgtacgacct gatcaggaaa agaacccggt
     1741 tacgtcaacg ggaggaagag gacagcatcg tggaggattt tccagataac agtgcctccg
     1801 ctttcgaacg gctgctcggc caggagagag agcgtcagat catga
//
LOCUS       DAOQUC010000897         1845 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_82476_length_1845_cov_3.173184, whole genome shotgun sequence.
ACCESSION   DAOQUC010000897 DAOQUC010000000
VERSION     DAOQUC010000897.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1845)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1845)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1845
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82476_length_1845_cov_3.173184"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..714)
                     /locus_tag="PKH07_13800"
     CDS             complement(<1..714)
                     /locus_tag="PKH07_13800"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HNT36057.1"
                     /translation="MNKDWTVWNFWPQGATDTTCVSALDVGFLYGDGLFETVRFEEGQ
                     LFFFQEHIDRLQRSCLALQIQADLSCLTKNALERLLEVSGLAEGVGRLKILVARGTDT
                     PTILAMARQEPKPLHRPVQCRVFPEQRSCPMARHKTLSYSYYWYARKWAQANGCDYAI
                     LVDPFDHVLEADMANLFLIEDDLIIRPFETLNRLVGVVEQVLLTRVFPELGLACEQRL
                     IQPEEITQECSLFLTNSMVD"
     gene            complement(711..>1845)
                     /locus_tag="PKH07_13805"
     CDS             complement(711..>1845)
                     /locus_tag="PKH07_13805"
                     /inference="COORDINATES: protein motif:HMM:NF012640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HNT36058.1"
                     /translation="AYYLDTRSSADPFVQLECLIKQSSMTAPHTAANPTVLCLGYDLK
                     NLIETLPMPKPSLQRVPDLYATDYWQNFSLRASRAGQLQDSILRCLSSSLTTDTCGAD
                     ALQASMTRQQYIGAVRRSLDYIFAGDIYQVNIAQRFTFPFTGSSWELFKRLYCLNPAQ
                     FSLFIRAREFDLIVCSPERLLKRSGTVLETRPIKGTRPRGETPQRDRDNLRELLLSQK
                     EKAELIMITDLHRNDLGKVSQYGMVNVVSKRHVHRMRNVFHTESIIRSVIRQDVSLSN
                     LLKALFPGGSVTGCPKLRAIEIIDELEPQARQFYTGAVGIINSEDDLDMAMTIRCITF
                     HRATGLAWFDIGSGIVAESDPESEYEETLDKAQSVFDVLGVKR"
BASE COUNT          455 a          465 c          480 g          445 t
ORIGIN      
        1 gtcaaccatc gagtttgtca gaaacaggga acactcctgg gtgatctcct ctggctggat
       61 caagcgttgc tcacaggcga ggccgagttc cggaaagaca cgggtcaaca gtacctgttc
      121 gacgacccca acaagtcgat tcagtgtttc aaagggtctg ataatcaggt catcttcaat
      181 aaggaagaga tttgccatat cggcctcaag aacgtgatcg aagggatcaa caaggatggc
      241 atagtcacag ccgttggcct gtgcccattt tcttgcgtac caatagtagg agtaactgag
      301 tgtcttgtgg cgtgccatag ggcatgagcg ttgttcggga aatacacggc attggacggg
      361 tcggtgtaaa ggcttgggct cctgacgtgc catagccaga atcgttggcg tgtcggttcc
      421 gcgggctact aaaatcttga gccttccaac accttcggca agtcctgaaa cctcaagaag
      481 acgctccaag gcattcttcg tcagacaact cagatcggct tggatttgta gtgccaggca
      541 agacctttgg agtcggtcaa tgtgctcctg gaagaagaac aactgcccct cttcaaatcg
      601 gactgtctca aaaagaccgt cgccatagag gaaaccgaca tccaatgcgc tgacgcaggt
      661 tgtgtcggtg gcaccctgag gccagaaatt ccagaccgtc cagtctttgt tcatcgtttc
      721 actcccaaca catcgaaaac tgactgtgcc ttatccagtg tttcttcata ttcactttcc
      781 ggatccgatt cggcaacaat tcccgaaccg atatcgaacc aggcaagtcc tgtggctcga
      841 tgaaatgtga tgcagcgtat tgtcattgcc atgtccaagt catcctcgga attgatgatt
      901 ccaaccgcgc cagtatagaa ctgtcgtgct tgcggttcca actcatcaat gatctcgatg
      961 gctctcaact ttggacatcc cgtaacagat ccgccaggaa acaaggcctt cagcaggttc
     1021 gaaagactca catcctgacg aatgacggaa cgaatgattg attccgtgtg aaagacgttt
     1081 ctcatgcgat gcacatgacg cttggatacg acattcacca ttccatactg ggagactttt
     1141 ccgagatcgt tgcggtgaag atcggtgatc atgatcaact ccgccttttc tttctggctc
     1201 agcagaagtt cacgaagatt gtcacgatcc cgctgaggtg tctccccgcg tggacgggtt
     1261 ccctttatcg gtcgggtctc caacactgtt cctgatcgct tcagaagcct ttccggtgaa
     1321 cagacaatga gatcgaactc ccgtgcgcga ataaacagag aaaactgcgc tggattcagg
     1381 cagtagagcc gcttgaacag ctcccatgag gaaccagtga acggaaacgt gaatctctgt
     1441 gcgatgttta cttggtagat atcccccgca aatatgtagt caagggatct tctcactgca
     1501 cctatgtact gctggcgtgt catggacgct tgaagagcat cagcaccaca ggtgtctgtt
     1561 gtgaggctgg aactcaaaca acggagaatg ctgtcctgca actggccggc tctgctggct
     1621 ctcagggaga aattctgcca gtaatcggtc gcatagagat ctggcactcg ttgtaatgag
     1681 ggcttgggca tgggaagcgt ctcaatgaga ttcttcagat catagccaag gcacagaacg
     1741 gttgggtttg cggcggtatg gggtgcagtc attgatgact gctttatcag acactccagc
     1801 tgcacaaacg gatcggcact ggaccgtgtg tcgagatagt aggcg
//
LOCUS       DAOQUC010000898         1843 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_82611_length_1843_cov_3.421141, whole genome shotgun sequence.
ACCESSION   DAOQUC010000898 DAOQUC010000000
VERSION     DAOQUC010000898.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1843)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1843)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1843
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82611_length_1843_cov_3.421141"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..339)
                     /locus_tag="PKH07_13810"
     CDS             complement(<1..339)
                     /locus_tag="PKH07_13810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202360.1"
                     /note="catalyzes the interconversion of 3-phosphoglycerate
                     and 2-phosphoglycerate; this enzyme does not require the
                     cofactor 2,3-bisphosphoglycerate as a phosphate donor;
                     BPG-independent PGAM; aPGAM; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HNT36059.1"
                     /translation="MRQIDLLSSLADESGTRILMVVMDGLGGLDFDGRGTALDVANIP
                     NMDVLASESACGLFDPVRPGITPGSGPGHLSLFGYDPLEFEIGRGILSALGVAFPVVA
                     GDLATTGKATP"
     gene            complement(576..923)
                     /locus_tag="PKH07_13815"
     CDS             complement(576..923)
                     /locus_tag="PKH07_13815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015949182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HNT36060.1"
                     /translation="MRLTPVILLICSNIFMTYAWYGHLKDFRTKPLLVVIVLSWGIAF
                     FEYCLQVPANRIGSTSFSLPQLKVMQEILTMLVFAGFCVLYMHEKLTLDYLWASFCLA
                     GAAFFMFRHLVLQ"
     gene            complement(1205..1279)
                     /locus_tag="PKH07_13820"
     tRNA            complement(1205..1279)
                     /locus_tag="PKH07_13820"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1245..1247),aa:Asn,seq:gtt)
     gene            complement(1575..>1843)
                     /locus_tag="PKH07_13825"
     CDS             complement(1575..>1843)
                     /locus_tag="PKH07_13825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36061.1"
                     /translation="FVELLEPLCGESTRDGIWHIPLIFRLSLNADDYPIWKAHNDLSR
                     TRNRAHSTTRSTRTGRIQHSDLSCRYLNRGIALYPIQNYLPRFI"
BASE COUNT          482 a          456 c          487 g          418 t
ORIGIN      
        1 cggtgtggct tttcccgttg tggcaaggtc tcctgccaca acgggaaaag ccacaccgag
       61 agcggacaga atgcctcgac cgatctcaaa ctcgagtgga tcatacccga aaagtgagag
      121 gtgccccgga ccactacccg gcgtgattcc tggtcgtact gggtcaaaca gaccacaggc
      181 tgattcagat gccagcacat ccatgttcgg gatgttggca acgtccaggg cggttcctcg
      241 accgtcgaaa tcgagtccac ccaagccgtc catgacaacc ataagaatcc tggtccccga
      301 ttcatccgca agacttgaca acagatcgat ctgcctcatt gatattcctc caccggctcc
      361 ctcttttcca agaggagtcc ttggcattgc tttctgcctt tcgcagtaga gagtaactta
      421 ccgatctggt cagtggattt caactcgaag atatttgcgg cagcgaatca gtcaacgaga
      481 acccgccaat tcactcagag aggacaagac tgtcagacaa atcggcctca gtccgagtcc
      541 tctcactgct gtccctcttt gccaagttgt ctttgtcact gaagcaccag atgtcgaaac
      601 ataaagaacg cagcgcctgc cagacaaaaa ctcgcccaga ggtagtccag agtcagtttc
      661 tcgtgcatgt agaggacaca gaagccggca aagacgagca tggtcagaat ctcctgcatc
      721 accttcagtt ggggaagtga gaaagaagtg ctcccgatac ggttggcagg tacttgcagg
      781 cagtactcaa aaaaggcaat cccccaactg agaacgatca cgaccagaag aggcttggtg
      841 cgaaagtctt tcagatgccc gtaccaggca taggtcatga aaatgttcga acagatcaag
      901 agaatcaccg gtgttagtcg catcgtatgc tccgtaaaaa tgatcgcgag gcgatatcag
      961 aacttgtatt cacgcatcgt ttgatatccg ttcttgtgaa ggctgtcaaa cggattcgca
     1021 gttcaacaat ccagcaagaa ccggtcggca ttgggcagac agtggttggc agtacatgaa
     1081 ttgcagaaag ttctcgatag agtgtatccc ctgttcaggg acagcagaaa tacaaagacc
     1141 ccgtaaggtc tggcctgggg gtcttcagaa atatacatca tgccaaaagc ggcgtgtgtg
     1201 actttggctc cccgggcaag actcgaactt gcagcccatc ggttaacagc cgattgctct
     1261 accattgagc taccgaggaa tagtatctca gcaaacgacc gagatatata gaacagctga
     1321 tacccttttg tcaagcaccg agaacgtttt ttggctatat tgctcagaga cgcaagaacc
     1381 gaacgagggt ggacgcagtg caacggtgtt ggaccagagt attcacccgg tttctccgga
     1441 gtcttcggcc aggtgactgg ggagcattgt tgatctttgt gcttctcata ttggcaagca
     1501 ttcgggtatt ctggtttgga tcggaaggca agacggttgt ctgtatcacg cctggcagaa
     1561 taatgaggtt tcccttagat gaaccgcggg agatagtttt gaatgggata caaggcgata
     1621 cctcggttga gatatcggca ggaaaggtca gagtgctgga ttctgcctgt ccgggtagaa
     1681 cgtgttgtcg aatgggcgcg atttctcgtg cgggacaagt cattgtgtgc tttccaaatg
     1741 ggataatcat ccgcattgag ggagagtcgg aagatgagag gaatatgcca gatgccatca
     1801 cgcgttgact ctccacacaa gggctccagg agttcaacga atc
//
LOCUS       DAOQUC010000899         1842 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_82698_length_1842_cov_2.777280, whole genome shotgun sequence.
ACCESSION   DAOQUC010000899 DAOQUC010000000
VERSION     DAOQUC010000899.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1842
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82698_length_1842_cov_2.777280"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            229..>1842
                     /locus_tag="PKH07_13830"
     CDS             229..>1842
                     /locus_tag="PKH07_13830"
                     /inference="COORDINATES: protein motif:HMM:NF019556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HNT36062.1"
                     /translation="MLNRRRSAELLPLVFFSLSVLLCGAEVPATALIPLSITQTLVVS
                     EGAKMMYKPVPFSLVEFDDNFWAPRLRRNREWSIPHVYWQLKEHGQLEAFRHKTDPTK
                     KTHIFWDSDLAKWMEGVSYCLMQQRDSELEATMDEIVSWMADAQLEDGYLNSHFIAAG
                     VENRWKNLRVEHELYCAGHLIEAAVAYYEATGKKRFLDIICRYVDHIDATFGSDKRRG
                     YPGHEEIELALVKLYRVTGEQKYLHLSQFFLEERGQQPHYYNQEALERGEDEQTANSR
                     THEELQAHKPIREQTEVAGHAVRAMYLYSAMADVAMEMGDDELFHVCEQLWENLTTKY
                     LYITAGIGSTSRHEGFTVPYDLPNENAYSETCASLGLVFWNHRMLQRDCDSRYADLME
                     QALYNSALSGVSLGGRLFFYTNPLASRGDRSRQPWFGCSCCPTNIVRFIASVGQYFYS
                     HNADQIAVHLYANGTASIPMGSQTVQLTQQTKYPWDGKVTLTLGLDAPAEFTLKLRIP
                     GWCKKASLRTAGKTISIQDCLQKGYVCMTR"
BASE COUNT          430 a          468 c          497 g          447 t
ORIGIN      
        1 cacccgagtg ggtgatcagt ctgtagcccc tgtgtcgatc tggtcggtct actgagatga
       61 cgacatctgg taaatgcata cggtgagcta tctatagaga tgaaaggtga cttggggaag
      121 ttacttcttc ttctgtaaca cgatggttgc tgccccgagt ccgaaagtga aacgctcttg
      181 tcctgctaga atgctctctg tgatgagatc acattaggag gcagaaacat gctcaaccgt
      241 cgtcggtctg cagaactgct cccgttggtt ttcttttctc tgagtgtttt gctgtgtggc
      301 gcagaagtac ctgcaacggc tctgataccc ctgagcatca ctcagacgct tgtcgtttcg
      361 gaaggagcaa agatgatgta caaaccggtt cccttcagcc ttgttgagtt tgacgacaat
      421 ttctgggcac ctcgtttgcg ccgcaacaga gagtggtcta tcccgcatgt gtactggcag
      481 ttgaaggaac atggccaact ggaagcgttt cggcacaaga ctgatcccac caagaagact
      541 catatcttct gggactccga ccttgccaag tggatggaag gagttagtta ttgtctcatg
      601 cagcagagag attccgaact ggaagccacc atggatgaga tcgtctcctg gatggctgat
      661 gctcagttgg aagacggata tctcaattct catttcatcg cggctggcgt cgaaaaccgt
      721 tggaagaacc tccgagttga gcatgaactg tactgcgccg gccatctgat cgaggcggcg
      781 gttgcgtact atgaagcgac gggaaagaag cgatttctgg atatcatctg tcgctatgtg
      841 gatcacattg atgccacctt tggttccgac aaacgtcgag gctatcccgg acatgaggaa
      901 atcgaattgg cgctagtcaa attgtatcgg gtgaccggcg aacagaaata tcttcacttg
      961 agtcagttct ttctcgaaga gcgcggtcag caacctcact attacaacca ggaagcactt
     1021 gagagagggg aagatgaaca gaccgcgaat tcgcgaacac acgaagagct ccaggcgcac
     1081 aaaccgattc gtgaacagac ggaggttgcc ggccatgcgg tgcgcgccat gtatctgtat
     1141 tcagcgatgg ccgatgtcgc catggagatg ggtgatgatg agctgttcca tgtctgcgaa
     1201 cagctgtggg agaatctgac caccaagtac ctctacatca ctgccggaat cggatctacg
     1261 tctcgccatg aaggatttac cgtcccctat gatcttccca acgagaatgc ctattcagag
     1321 acatgtgctt ccctggggct tgtcttctgg aaccaccgga tgctgcagcg tgactgcgat
     1381 agtcgctatg cagacctgat ggaacaggct ctctacaatt ccgcattgag tggcgtgtct
     1441 ttgggcggac gtctcttctt ctacaccaat ccccttgcga gccgaggaga cagaagccgg
     1501 cagccttggt ttggatgctc ctgttgtccg accaacatcg ttcgcttcat cgcttctgtg
     1561 ggacagtatt tctattctca caacgctgac cagatcgctg tgcatctcta tgcaaatggt
     1621 accgcctcga tcccaatggg aagtcagact gtgcaactca cccaacagac caagtatccc
     1681 tgggatggaa aagtgacgct cactctcggt cttgacgcgc ctgcggagtt caccttgaag
     1741 ttgcgcattc cgggctggtg caagaaggca agtcttcgaa ctgccgggaa gaccatctcc
     1801 atacaggact gcctgcagaa gggttacgtc tgcatgacgc gg
//
LOCUS       DAOQUC010000900         1841 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_82748_length_1841_cov_3.496081, whole genome shotgun sequence.
ACCESSION   DAOQUC010000900 DAOQUC010000000
VERSION     DAOQUC010000900.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1841)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1841)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1841
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82748_length_1841_cov_3.496081"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(109..816)
                     /locus_tag="PKH07_13835"
     CDS             complement(109..816)
                     /locus_tag="PKH07_13835"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNT36063.1"
                     /translation="MAFEALDQIGMTDLFSDLGKRMNLPQGVFYWSGRAKKESKINAT
                     IGSAAGFDSEIGLGDGDKFITFHLPMIGNEVPGLNSEQIFPYAPIAGLPELRKAWKQW
                     ILRKCGAAADGLEPLLTLPQVLPGITGALTYTLELFVNPGEAVVSPEKRWGNYDNTVI
                     RRLNARFQEFPLFVKDEFNVEGMVGSIQEVWKTQNRAVVVLNFPNNPTGYQPTARINE
                     EILNGLLDLVNKHDKKV"
     gene            complement(816..1667)
                     /locus_tag="PKH07_13840"
     CDS             complement(816..1667)
                     /locus_tag="PKH07_13840"
                     /inference="COORDINATES: protein motif:HMM:TIGR00099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HNT36064.1"
                     /translation="MKYQIIALDIDGTLLGSDNKISPATRTALDRAYHNGLKVILCTG
                     RRFRTARRFSIELGFDAPIVTNCGALIKNPQTEETLYRNALDVSRCEALMHFLLERSD
                     SVVTYLDTPLDTNRDFVICDRASSSASFQKYLAIHRGLYYLLPDGRLPEGSIPIGICV
                     IEDRERLNQIEMEVQKHFGSSVQEHIMGSVPVLGPVLEMFAPGTTKWNALCTWAETLD
                     VHPDGIVAAGDEMNDMEMISKAGLGAAMGNARQEIKDVADLVLPSNDEDGIVALVQHL
                     LESMEEK"
BASE COUNT          399 a          521 c          468 g          453 t
ORIGIN      
        1 cgctgcctct tgtgagtttt tctcgtaaac ttctaagaag cgctttagca ggcgcaaaaa
       61 gtccggattc tcaagatcct ttggaagttt ttcgccgccg tcactcattt aaaccttctt
      121 gtcatgctta ttcaccagat ccaagagccc gttaaggatc tcctcattga tccgtgcggt
      181 cggctgatac ccagtcggat tgtttggaaa gttgagcacc acgacagccc gattctgagt
      241 cttccagact tcctgaatac tgccgaccat accctctaca ttgaactcat ctttcacaaa
      301 gagggggaac tcctgaaagc gcgcattcaa gcgccgaatg acggtgttgt cgtagttgcc
      361 ccagcgcttt tccggactga cgacagcctc tcccggattg acaaagagct caagcgtgta
      421 agtgagcgca cccgtgatgc cgggaagcac ctggggaagc gtcaagaggg gctcaagacc
      481 atctgccgct gcaccacact ttctcaggat ccattgcttc caggccttgc ggagttcggg
      541 aagtcccgcg atgggcgcat agggaaatat ctgttcggag ttcagaccag ggacttcgtt
      601 gccgatcatc ggaagatgaa acgtgatgaa cttgtctcca tcgcccagcc cgatctcgct
      661 gtcgaatccg gcggcggagc cgatcgttgc gttgatcttc gattctttct tggcgcgtcc
      721 gctccagtag aagacccctt ggggcaggtt cattctcttg cccaagtcag agaaaagatc
      781 cgtcattcca atttgatcca atgcttcaaa agccatcact tttcctccat gctctccagc
      841 agatgctgta ccaatgcaac gataccgtct tcatcattac tcggcagaac caagtctgcc
      901 acatccttaa tctcttgtcg tgcatttccc atggccgccc ccaggccagc cttggaaatc
      961 atctccatat cattcatttc atcgcccgcc gccacgatgc cgtcaggatg gacgtcgagc
     1021 gtttccgccc aggtgcagag ggcgttccat ttcgttgttc ccggcgcgaa catttccaga
     1081 acaggtccca gcaccgggac ggatcccatg atgtgctcct ggacgctgga gccgaagtgc
     1141 ttctgcacct ccatctcgat ctggttgagg cgctctctgt cttcgatgac acatatccca
     1201 atgggaatgg aaccttcagg gagcctgcca tcggggagca aataatacag accgcgatga
     1261 attgccaagt atttctgaaa agaagcgctt gatgacgcac gatcacaaat cacgaaatct
     1321 cggttggtgt ccaacggggt gtccagatag gtcaccacag agtcgcttcg ctccaggaga
     1381 aagtgcatca gcgcctcaca acgcgataca tccagagcgt ttcgatagag ggtctcttcc
     1441 gtctgcggat ttttgatgag cgcaccgcag ttggtgacga tgggggcgtc aaaaccaagt
     1501 tcgatgctga accgccgtgc cgttctgaac cgacgtcctg tgcaaagaat gactttcagt
     1561 ccgttgtgat acgccctgtc cagtgcggtt cgtgttgccg ggctgatctt gttgtcggaa
     1621 ccgagaagcg ttccgtcaat gtccaacgcg atgatctggt atttcattcg tcttcgtagg
     1681 tgacagccaa tacttcttcg ggtgtcgtga caccctggcg catcttcttc agggcattct
     1741 gtcgaagggt gaccatacct tgttggatgg ccgtcgcacg aatctcctcc agagaaggat
     1801 tggcgacgat gcagcgccga atggcttccg tcatctcgat g
//
LOCUS       DAOQUC010000901         1839 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_82931_length_1839_cov_2.503363, whole genome shotgun sequence.
ACCESSION   DAOQUC010000901 DAOQUC010000000
VERSION     DAOQUC010000901.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1839)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1839)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1839
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82931_length_1839_cov_2.503363"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1749)
                     /locus_tag="PKH07_13845"
     CDS             complement(<1..1749)
                     /locus_tag="PKH07_13845"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HNT36065.1"
                     /translation="MSAWYQGHTTHPNNASEFAGMLSALNRVPDESWYFNESTTPMSP
                     YPGEAGTSDGLRGGYVAKLQTAIWYDEATDFDLTWRTDDAAELYLNGNLIASGYGSCG
                     ACAWRTTRLNFQAGWNILTLWYEEVAGGDYFEHSTLLPSDRMFAVADWEMEYWRDNHT
                     THPTDIGGFESEMAAKLNQTRTATIFVEEPIRRFMPTFTTAGTADDSITRTRYYAHLQ
                     SRIRETNGRLYDWDIMADDASAVYANGSRTSYRIGCCAWYDTIFTLSAGTNQIDMWYE
                     EVLGGDYIMNDDNDVLPAMGSCLGTFPLCTVTRLDANPTSSSTVHFRVDFDENVSGFT
                     QSDVVLSGTAPGKSITAFTGSGKQFTVTVGNIGGSGTVTLMVPVGGGFDTLGNPNDHG
                     SPVTYDVNMGPTDTPTNTMQPTDTPTNTPSFTPSNTPSNTPTFTWTNTPTETPTNTPT
                     FTPTFTPTNTPTMTPTNTPTSFITDERTILTDADTHIRDYNTDYMARNYGVYNRLLIG
                     YYNGFGFYHQRSLVRFGISSIPSNATIVEAKLSLYQDVGSSHASSNQDHVIHLYRLTS
                     SWVEGTQNGAAGSVCWN"
BASE COUNT          404 a          477 c          494 g          464 t
ORIGIN      
        1 attccagcat accgaacccg ctgcaccgtt ctgagtccct tcaacccagg aacttgtaag
       61 ccgatacaga tgtatgacgt ggtcctggtt gctgctggcg tgagatgaac caacatcctg
      121 atagagactg agttttgctt ccacgatggt ggcattgctc ggaatcgaag agattccgaa
      181 tcgcaccaga gatctctgat gatagaagcc gaaaccattg tagtagccaa tcagaagtct
      241 gttgtatacc ccatagttgc gagccatgta gtcagtgtta tagtcgcgaa tatgagtatc
      301 ggcatctgta agaatcgttc tctcgtccgt gatgaacgag gttggtgtat ttgtcggggt
      361 catcgtaggt gtgttcgttg gagtgaacgt tggagtgaac gttggtgtat tcgttggcgt
      421 ttccgtcgga gtgttcgtcc aagtgaaggt cggagtgttc gatggggtgt tgctgggagt
      481 gaacgacggc gtattggttg gagtatctgt tggctgcatg gtattcgtag gcgtgtctgt
      541 gggtcccatg ttcacgtcgt aggtgaccgg actgccatgg tcgtttggat tgcccaaggt
      601 gtcaaaacca ccccccaccg gaaccatcaa ggttaccgtg ccacttccac ctatattacc
      661 tacggtgacc gtgaattgtt tcccgctgcc agtgaatgcg gtgatactct ttcccggggc
      721 ggttccagat agaaccacat cagactgggt gaaaccgctc acattctcat caaagtcaac
      781 tcggaagtga acagtcgaac tgcttgtcgg atttgcgtcc aagcgggtaa ccgtgcatag
      841 gggaaacgtc ccaaggcaac ttcccatggc aggcaacaca tcattgtcgt cattcatgat
      901 gtagtcgcct ccaagaactt cttcatacca catatcaatc tggttcgtac cggcgctcaa
      961 cgtaaagatg gtgtcatacc aggcacagca cccgatacga tacgaagttc ttgaaccatt
     1021 ggcgtacact gcagacgcat cgtctgccat gatatcccag tcgtagagac ggccgttcgt
     1081 ttcccgaatc ctgctctgta agtgagcgta gtagcgcgtt cgggttatgc tgtcgtcagc
     1141 cgttccggcg gtcgtgaaag tcggcatgaa ccgtcgaatc ggctcctcga caaaaatcgt
     1201 ggctgtgcgc gtctggttca gtttggccgc catttcggac tcaaaccctc caatgtctgt
     1261 cggatgggtt gtgtgattgt ctctccagta ttccatctcc cagtcggcaa cggcaaacat
     1321 tcggtctgat ggaagcagcg tactgtgctc aaagtagtca ccaccggcaa cttcttcgta
     1381 ccagagtgtc aaaatgttcc atccggcttg aaagttcaac cttgtcgttc tccaggcgca
     1441 cgcaccacag gacccgtagc cggacgctat gaggttccca ttgagataca gttcagcggc
     1501 atcgtcagtc cgccatgtca ggtcaaagtc tgtcgcctca tcataccaga tggcggtctg
     1561 gagttttgcg acgtagcctc ccctcagtcc gtcacttgta cctgcttcac ccgggtacgg
     1621 agacatcggt gtggtgctct cattgaagta ccaggactca tcaggaactc gattcagtgc
     1681 actgagcata cctgcaaact ctgatgcgtt gttagggtgc gtcgtatgtc cctggtacca
     1741 ggcgctcatc cgccagtccc tatgtaaggg tggctcggca agttgagccc ctccagcaat
     1801 ggagcctggg aaaggagaat gccagaaaca atcgccacc
//
LOCUS       DAOQUC010000902         1838 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_82987_length_1838_cov_2.796971, whole genome shotgun sequence.
ACCESSION   DAOQUC010000902 DAOQUC010000000
VERSION     DAOQUC010000902.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1838
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82987_length_1838_cov_2.796971"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..720)
                     /locus_tag="PKH07_13850"
     CDS             complement(<1..720)
                     /locus_tag="PKH07_13850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36066.1"
                     /translation="MDKRLTRCSVVILGAFVLCGNAFAQWATQTVNLHAGWNAIFLEL
                     EPYPQQCEFQLEGLPILSVWTCDQVSSPMQFIQNPDELLPEMPEWRYFFPPASPESFA
                     TNLRDFQAGRCYLIRTSQAATWNIIGKPKLTQQRWKPDSYNLVGCYVDETAPPTLATW
                     FSGSPAHTPLDVWEFGADDRWHQITSPTTASVQRGIAYWVYCKGASDYQGPVTVNLSG
                     EDILTYQRLTTQKQVEVSHNAL"
     gene            complement(1061..>1838)
                     /locus_tag="PKH07_13855"
     CDS             complement(1061..>1838)
                     /locus_tag="PKH07_13855"
                     /inference="COORDINATES: protein motif:HMM:NF015649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bacterial transcriptional activator
                     domain-containing protein"
                     /protein_id="HNT36067.1"
                     /translation="LFAFLRAVRQVADDPEHKGERVLWMAALCGLCVFLFHSLVTFPM
                     QMPANRIVALLFLAISTAPAASEHKACPVVREGRRQRFSPTMVLAMAILTFGVAATIV
                     RPIISDHLLWKALRASNPVRRFYLERAVDWNPDSWTNWFYYGEALRAEGKTSAAVMAW
                     EHAAQLEERLVTHELLYRAYLELGRGSDLVRQAEAIVRLNPCYPPNQLRLGDEYKRMG
                     RSNEALKAYREALRLDPSLEGIVVTKTRSLIQDTGQGDDS"
BASE COUNT          432 a          499 c          464 g          443 t
ORIGIN      
        1 caatgcgttg tggctgactt caacctgctt ctgtgtcgtc aggcgctgat aggtcaggat
       61 gtcttctccg gaaagattca cggtaactgg tccctggtaa tctgaagctc ccttgcagta
      121 gacccaatag gcgatccccc tctggacgga agcggttgtc ggactggtaa tctggtgcca
      181 ccggtcgtct gcgccgaact cccagacatc caagggtgtg tgcgctggag agccgctgaa
      241 ccatgtggcc agagttggtg gcgcggtttc gtcaacatag catccaacga gattgtatga
      301 atccggtttc catctttgtt gtgtcagttt cggtttgccg atgatattcc atgtggctgc
      361 ttgagaagtt ctgatcagat agcatctgcc tgcctggaaa tctctgaggt tcgtggcaaa
      421 ggattcaggg cttgccggtg ggaaaaagta tcgccattcg ggcatctccg gcagcagttc
      481 atcagggttc tggatgaact gcatcggcga actcacctga tcgcaggtcc agacactcag
      541 aatgggcaga ccttccagtt ggaactcaca ctgctgagga tatggttcca gctcaagaaa
      601 gatggcgttc catccggcat gcaaattgac cgtctgggtt gcccactgcg caaaggcgtt
      661 tccacagagc acgaaggctc ccaatatcac cacagagcac cgtgtcagtc ttttgtccat
      721 cttcttgctc ccttatctga tcaagatctt cgtatggcta ggttttcaga aacacttaag
      781 aattgcccca agtaccaaga aagccccttt cgagaaccaa tacaacacaa gtatccttcg
      841 aaatcatcat tgggaaaaag gcacaatact cctcagttac tccgatagga cggataccta
      901 ccccctttga aaagccagat gaacctatag ccgtattgtt aatctgattc tagcattctc
      961 taacatcttg tcaaggtttg gatcgtgctt ctgagaattt cggcagctcg atatttcccg
     1021 aggagaggat tagggcgtcc tgctctgctg gacggagccc ctacgaatcg tctccctgtc
     1081 cggtatcttg gatgagcgac ctggtctttg ttacgacgat tccctcaaga gaaggatcca
     1141 gtcttagcgc ctctcgatag gctttgagtg cctcattgga gcgacccatt ctcttgtatt
     1201 catcgccaag ccgaagttgg tttggcggat agcagggatt cagccgaaca atggcttcag
     1261 cttgcctgac aagatcgctt cccctcccca attccaaata cgcccgatag agcagttcgt
     1321 gcgtcacaag tcgctcttcc agttgcgccg cgtgttccca agccatcacg gcagccgacg
     1381 tctttccttc agccctcaag gcctctccat agtagaacca attcgtccag gagtctggat
     1441 tccagtcaac cgcacgctca agatagaaac gacgaaccgg attgctcgct cgaagtgctt
     1501 tccagaggag gtggtcagag ataatcggcc tgacaatggt ggcggcaacg ccgaacgtca
     1561 gtatcgccat ggcaagcacc attgtgggac tgaacctctg tcgtctgcct tcccgtacaa
     1621 ccggacaggc cttgtgttca cttgcagccg gagcagttga aatggccaaa aagagtaaag
     1681 cgacaatccg atttgcgggc atctgcatgg ggaatgtcac cagactgtgg aaaaggaaaa
     1741 cacaaagtcc acacaaggca gccatccata gaactcgctc ccccttgtgc tctgggtcgt
     1801 ctgccacctg acggactgct cgcagaaatg cgaacaga
//
LOCUS       DAOQUC010000903         1837 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_83041_length_1837_cov_3.900112, whole genome shotgun sequence.
ACCESSION   DAOQUC010000903 DAOQUC010000000
VERSION     DAOQUC010000903.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1837
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83041_length_1837_cov_3.900112"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /locus_tag="PKH07_13860"
     CDS             <1..343
                     /locus_tag="PKH07_13860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36068.1"
                     /translation="PHVLFWLQSSLKRVFPNSEPGTETTLTLLSARNRQISFQACLRN
                     NRPYPLNVVCSVTGTEDLRIQIRRVGYVPLRHMTIETDPTELEGIGHIPGMAPDPLFP
                     ENSAVVGPWEN"
     gene            386..1603
                     /locus_tag="PKH07_13865"
     CDS             386..1603
                     /locus_tag="PKH07_13865"
                     /inference="COORDINATES: protein motif:HMM:NF024716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HNT36069.1"
                     /translation="MRNLQVEYLFHEGTQKAELTAKVDVRPLVLQKRSDFPVTHWWRL
                     ESLYEYYKVDMNSDRCRQLTRAYLKNLVDHGNNMIFVPAWFPRSEVVARPAQLLGIVE
                     TTPNNYEFDWTRVREFVEQARECGAEAFEWPHLWIYWGVKHPERFYTQKSDGSWQLLF
                     EPDSDGFGDRYVGFLKQLMASWHRFLLDERILEISYFHLSDEPGGDEHFANYTRARQL
                     LREIAPWMKVLDALSEVRYGKENLTDYPIPIISSAPDYIREGIPHWVYYCCGPRGAYV
                     NRFMDTPLIKIRMTGWLFYRLKAQGFLHWGYNGWHVIEKDAIVDPFTQFDAGWYPGIP
                     NGDPFVVYPGENGPIDSIRWEVFFESLQDFALLQTAGIDRDDALLDAIKSYADFPKSE
                     DWLEEAIASVLSK"
     gene            1624..>1837
                     /locus_tag="PKH07_13870"
     CDS             1624..>1837
                     /locus_tag="PKH07_13870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36070.1"
                     /translation="MKLTDVSVHPFGAFLDTPASFAIHRYDLPRNWDYIYTNANTLLR
                     INHDGTGYLQVDPPAGPALFKMERLEK"
BASE COUNT          420 a          463 c          496 g          458 t
ORIGIN      
        1 accgcatgtg ctgttttggc tgcagagttc actcaagcgt gtctttccaa actcggagcc
       61 tggaaccgaa accaccttga ctctgttgag cgcgagaaac agacagattt cgtttcaggc
      121 ctgtctgcgc aacaacagac cctatccgtt gaatgttgtc tgctctgtca ctgggacaga
      181 ggacttgaga atccagattc gccgtgtggg atatgtgcct cttcgtcaca tgaccattga
      241 gacagatcca acggagttgg aaggcatcgg gcatattccg ggaatggctc ccgatccatt
      301 gtttcctgag aactcggctg tcgttgggcc gtgggaaaac tagtcctttt ggatcaccat
      361 caacgtacct gcggatgtgc ccggtgtgcg caatctccaa gtcgagtatt tgtttcatga
      421 aggcacgcag aaagccgagc tgaccgcgaa agtggatgtc cgaccactgg tactccaaaa
      481 gcgcagtgat tttccggtca cacattggtg gagactggag tctctctacg aatactacaa
      541 ggtggacatg aacagcgacc gatgtcgcca gttgacgcga gcatatttga agaaccttgt
      601 ggatcatgga aacaacatga tcttcgtgcc ggcatggttc ccacgaagtg aggttgttgc
      661 ccgaccggcg cagcttttgg gaattgtaga aacaacaccg aacaactatg agtttgactg
      721 gacacgtgtt cgggaatttg ttgaacaggc tcgtgagtgt ggtgcggaag ccttcgagtg
      781 gcctcatctg tggatatact ggggcgtcaa acatcccgaa cgcttctaca cgcagaagag
      841 cgacggttca tggcaactgc tcttcgagcc cgacagtgac ggatttggag acaggtacgt
      901 cggattcctc aagcagttga tggccagctg gcaccggttt ctactggacg agagaattct
      961 ggagatttcg tactttcatc tttctgatga acccggcggt gatgagcatt ttgccaacta
     1021 cacgcgagcg cgccaactgc ttcgcgagat cgctccctgg atgaaagtcc tcgatgctct
     1081 cagcgaagtt cgctacggca aggagaacct gactgactat ccgataccga tcatcagctc
     1141 tgcacctgat tacatcagag agggcatccc gcattgggtg tactattgct gcggcccacg
     1201 cggtgcttat gtgaaccggt tcatggacac acctttgatc aagattcgca tgaccggatg
     1261 gctcttctat cggctcaaag cacaaggatt tcttcactgg ggatacaacg gttggcacgt
     1321 gattgagaag gatgccattg ttgatccctt cacccagttt gatgccgggt ggtatccagg
     1381 gatccccaac ggcgatccgt ttgttgtcta tcctggggag aacgggccca ttgattcgat
     1441 tcgttgggaa gtattctttg aatcattgca ggatttcgcg cttcttcaaa ccgccggcat
     1501 tgaccgggat gatgcgctct tggatgccat caagtcctat gctgattttc caaagtccga
     1561 agactggctc gaagaggcga ttgcgagcgt tctttcgaaa taggccaggg gttggagtca
     1621 cccatgaaac tgacagacgt ttctgtgcat ccgtttgggg cgtttcttga cactcctgca
     1681 tcgtttgcga tccaccgtta tgatcttccc aggaattggg attacatcta caccaatgcc
     1741 aacacgctac ttcggatcaa ccatgatggg acgggatact tgcaggttga tccgccagct
     1801 ggccccgctc tcttcaagat ggagcgactt gaaaaac
//
LOCUS       DAOQUC010000904         1834 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_83268_length_1834_cov_3.265880, whole genome shotgun sequence.
ACCESSION   DAOQUC010000904 DAOQUC010000000
VERSION     DAOQUC010000904.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1834
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83268_length_1834_cov_3.265880"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1834
                     /locus_tag="PKH07_13875"
     CDS             <1..>1834
                     /locus_tag="PKH07_13875"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HNT36071.1"
                     /translation="SVTAQGYAPGYKRGVLVPNPDVSIALFKGTAVTGRVLAQTDGGT
                     EPAPGATVLARSDDARIAAQTVAGQDGSYQIDALAPGRYELLAALEGATSVDLNSQRG
                     KPIEIARGQTQPVQADLILHAGGTLKGTVTDTRATPLSDASVDLYMMRKSNQQWELWN
                     SLTTDSSGAFAFVGLPSGRYYMRVRKANFGELRYSSEYIIGLGESLDGIMLQLQAAAA
                     IGGTVTEQGGYPLPGVLVRVEGSDCSGVSDAEGNFSLNCVSQDFGQVNLIFSLIGYEN
                     KKVMDVSVGTNDLAVTLRRGGIVRGRVTDKESGASVAGAILRLWELDLSGNRSQVGQN
                     PTASTGSDGVYEFRGVTLEVPFEIEVSCEGFEPQKKEDFMGTEGQLTLDFQLESGCVL
                     TGIAMNLKDSQPIPDVEIRAAYGDKVKRVYTDSAGEFQISGLPRGQITLRVYHREFGS
                     SFSVKSDEQVRDFQIVTLSGAETDVSIYMRRGVALRGTVTARSSGEPVGGVQISEIAS
                     GRFTQTGQDGFYLLDGLSAQQGLRLAIVADGYEEIFLDPQKLSFPIGMHDLSFQVGRG
                     GTIRGHVTPMSGDYKIFLQRVNRGDEEEFYARAYCTQPDWEYAIP"
BASE COUNT          406 a          466 c          571 g          391 t
ORIGIN      
        1 ctccgtcacc gcacagggtt atgcgccagg ctacaaacgt ggcgtcttgg tacccaatcc
       61 ggatgtgtcc atcgcgctct tcaaaggaac ggcggtcacg ggacgcgttc tcgcccagac
      121 ggatgggggc acggaaccgg ctcctggagc cactgtactt gcccgaagtg acgatgcccg
      181 aatcgctgcg cagaccgtgg caggacagga cggcagttac caaattgacg ctctggctcc
      241 cggacgatac gaactcctgg cagcgctgga aggggcgact tcggtagatc tgaattccca
      301 gcgggggaaa ccgatcgaga tcgcccgagg gcagacacaa ccggttcagg cagatttgat
      361 cctgcatgcg ggtggcacgc tgaagggaac agtcacagac acgcgagcga ccccgctgtc
      421 ggatgcctca gtggatctct acatgatgcg aaagtccaat cagcaatggg aactctggaa
      481 ttccctcacg acagactcca gtggtgcctt tgcttttgtc gggcttccca gtggccgcta
      541 ttacatgcgg gtcagaaagg ccaattttgg tgagctccgc tacagttccg agtacatcat
      601 cggtttgggg gaatcgctcg atggaatcat gcttcaactc caagccgcgg cagccattgg
      661 cgggacagtg accgagcagg gcggatatcc tcttcccgga gtcctggtgc gcgtggaggg
      721 ttcagattgt tcgggtgttt cagatgccga ggggaatttc agccttaatt gtgtcagtca
      781 ggacttcgga caggtcaatc tgatcttcag cctgatcggt tatgagaaca agaaggtaat
      841 ggatgtgtcc gtcggaacca acgatcttgc ggtgacgctc aggcgtggag gcatcgtccg
      901 aggccgtgtg accgacaagg aatcgggggc atcggttgct ggtgcaattc tgagactttg
      961 ggagcttgat ctgtccggca atcgctcaca ggttggtcag aatcccacgg cctccacggg
     1021 ctcggatggg gtctatgagt tccggggagt gacattggaa gtcccctttg agatagaggt
     1081 gtcttgcgaa ggcttcgaac cgcaaaagaa ggaagatttc atgggcaccg aaggacagtt
     1141 gacgctggat ttccaattgg agtccggctg tgtcctgact ggtattgcga tgaacctcaa
     1201 ggacagtcag ccgatacccg acgtggagat tcgtgccgcc tacggtgaca aggtcaagag
     1261 agtgtataca gacagcgccg gagagtttca gatttcgggt cttcccagag gccagatcac
     1321 tctgcgcgtt tatcatcggg agtttggaag cagtttctct gtcaagtctg atgagcaggt
     1381 gagagatttc cagatcgtta cattgtccgg agctgaaacc gacgtctcga tctacatgag
     1441 gcgcggcgtc gctctgaggg gcacggtcac cgcgaggagt tccggggagc ccgtgggggg
     1501 agtgcagatt tccgagattg ccagtggccg attcacccag acagggcagg acggcttcta
     1561 tcttctggac ggcctgtcag cacaacaagg cttgcgattg gcgatcgtcg cggacgggta
     1621 tgaagagata tttctcgatc cccaaaaact gagtttcccg ataggaatgc atgatctcag
     1681 ttttcaggtc ggaaggggcg gcacaatccg aggtcatgtc actccgatga gtggcgacta
     1741 taagatattt ctccagcgcg tcaacagagg ggatgaggaa gagttctacg ctcgagcgta
     1801 ctgcacacaa ccagactggg agtacgcaat accc
//
LOCUS       DAOQUC010000905         1831 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_83474_length_1831_cov_3.860923, whole genome shotgun sequence.
ACCESSION   DAOQUC010000905 DAOQUC010000000
VERSION     DAOQUC010000905.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1831)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1831)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1831
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83474_length_1831_cov_3.860923"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..380)
                     /locus_tag="PKH07_13880"
     CDS             complement(<1..380)
                     /locus_tag="PKH07_13880"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HNT36072.1"
                     /translation="MNSKDPQIQSLRQREAYRSQPDYVTFIPEYTSEGIPESGNEHFL
                     VFESAKGALMAVWTSRNEPMPNIPPGEGPNNHILFSKSLDGGVTWETPKHLAGPRPHE
                     YHSHIANWAFPMVSQTGRIYVLWN"
     gene            689..1087
                     /locus_tag="PKH07_13885"
     CDS             689..1087
                     /locus_tag="PKH07_13885"
                     /inference="COORDINATES: protein motif:HMM:NF012610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HNT36073.1"
                     /translation="MTAFTDWRVDFSTGIPVYKQIVNYLFLEIGRGSLREGDRLPTIK
                     ELAEQLNVNPNTVAKAYRELDIKGIIAGRRGDGSYVSAQPEHPVSKTTQQKRVKLDEL
                     FGRVIAEAKAYGITEADILEHIAERIHEDE"
     gene            1080..>1831
                     /locus_tag="PKH07_13890"
     CDS             1080..>1831
                     /locus_tag="PKH07_13890"
                     /inference="COORDINATES: protein motif:HMM:NF039362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6159 family protein"
                     /protein_id="HNT36074.1"
                     /translation="MSRFQRSWLLFKSSVSVIRRNPKLLLFPIATSLMVCVIMLFFLT
                     PLVFWNTGHGITDAGHWKTLGSYFLTASDDGEELHFNPIGYLVVIVIYFVSVFLATFS
                     NVAFFNEILNSLNGRSVSIMGGLKFATTRLKAIFLWSLFAGTIGLIIKMLEERVGFIG
                     RLVLRFIGIAWSVACVFVVPAIVREEEHSSPILFLRNSAATLKRTWGESLIGYLGIQF
                     GTVLVLLISIFLLIGGAIVSAVLSNFWIIAVVA"
BASE COUNT          409 a          417 c          518 g          487 t
ORIGIN      
        1 tgattccaga gcacgtagat tcgtcccgtt tgggagacca tagggaaagc ccaattggcg
       61 atgtgtgaat gatattcatg ggggcgaggg cctgcgagat gcttcggggt ctcccaagtg
      121 actccgccgt cgagagactt ggagaagaga atgtgattgt tgggaccttc tcctggtggg
      181 atgttgggca ttggctcgtt tcggctcgtc cagaccgcca tcaaagcgcc tttggcactc
      241 tcgaaaacca gaaagtgctc gttgccggac tcggggatgc cttctgaggt gtattcggga
      301 atgaatgtaa cgtagtcagg ctggcttcga taggcctctc gttgtctgag cgactggatt
      361 tgtggatctt tagagttcat gggatacgct tctcctgaat cattttgact gatgctatca
      421 ctcgggggtg agacaatcaa tgaagttgct ctgcaggatg cgaagcattt gtgtcccctc
      481 tctggaggaa cttgccttcc aggggaatcc gattgttggc ccacaatgac aaacctgctg
      541 tggagcgtgg agaagagttc tattgtttct gaatagagaa agggtgttgg tgttgcatta
      601 gggtgttttt ttctggaatg aagaatttcg cttgacaagg aaattattct attttactac
      661 tatattagta ttttatgaag gaatctcaat gactgcattc acagactggc gcgtagactt
      721 ctccaccggg attccggtgt acaagcagat cgtaaactat ctctttcttg agatcggacg
      781 aggttcgctt cgcgaaggag acaggctgcc gacgatcaag gagctagccg agcaactgaa
      841 cgtcaacccg aatacagttg caaaggccta ccgagaactg gatatcaagg ggatcatcgc
      901 tgggcgtcgg ggcgatggct cttatgtctc agcacagccg gagcacccgg tttccaaaac
      961 gacacagcaa aagagggtga agctcgacga actgttcgga cgagtcatcg cggaagcaaa
     1021 agcatacggt attacggaag cggatattct ggagcatata gcggaaagga ttcacgaaga
     1081 tgagtaggtt tcagagaagt tggttgctgt tcaagagttc agtgtcggtg atacggcgaa
     1141 atccgaagtt gcttcttttc ccaatcgcca cgtcgctcat ggtctgcgtc atcatgcttt
     1201 tcttcctgac gcccctggta ttctggaata cgggtcatgg gatcaccgat gcaggccact
     1261 ggaagacgct cgggagttac tttctcacgg ccagcgatga cggagaggag ttgcacttca
     1321 acccaatagg ctatcttgtg gtcatagtca tctacttcgt ctccgtattc cttgcgacct
     1381 tttccaatgt cgcattcttc aacgagattc tgaactcgct gaacggccgt tcggtatcca
     1441 ttatgggggg actcaagttc gccacgacga ggctcaaggc catctttctc tggtctttgt
     1501 tcgccggtac gataggtctt atcatcaaga tgttggaaga gcgggtcggc tttatcggaa
     1561 gacttgtgtt gaggttcatc ggaatcgcct ggagtgtggc atgcgttttc gtcgtgccgg
     1621 cgatcgttcg ggaagaagag cattccagcc ccatcctgtt tctacgaaac tcggcagcga
     1681 cgctgaagag gacttggggg gaatcgctca tcggttacct tgggatccaa ttcggcacag
     1741 tgctcgttct gctcatctcc atattcctcc tgatcggcgg tgcgattgtc agtgcagttt
     1801 tgagcaactt ttggataatc gccgtagtcg c
//
LOCUS       DAOQUC010000906         1827 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_83798_length_1827_cov_2.878104, whole genome shotgun sequence.
ACCESSION   DAOQUC010000906 DAOQUC010000000
VERSION     DAOQUC010000906.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1827
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83798_length_1827_cov_2.878104"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1827)
                     /locus_tag="PKH07_13895"
     CDS             complement(<1..>1827)
                     /locus_tag="PKH07_13895"
                     /inference="COORDINATES: protein motif:HMM:NF027656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4962 domain-containing protein"
                     /protein_id="HNT36075.1"
                     /translation="AGEKEGSTTQLTGWSTVRSFTLTRKAAVFPQPTSEMLKEMIPLD
                     HPRLFLRPEDVAQVKARCEGDLKEIWTELVASADKLIEKPPDSTEPPLYPEGTVRLSE
                     VWKNIWWGNRTRTVAVGNAASTLAFAYRIAGDEKYARAARELLMAFCEWDPNGATNYQ
                     YNDEAGMPALYLPARAYTFLGDFLTEQERSRVIEVMNIRGRHVFEYLTGNRHHWRPYA
                     SHSNRAWHKMGELAMAFYHDIPEASVWLDYAMTIFYTAYPVWGDSQGGWHEGTAYWSS
                     YMSRFFYWADVMQSILKINIYDRPFYHQAGYYPLYVVPPGTGTGGFADQAILINSKSL
                     APVVMELAQGASNPYWLWYAESQGHRPSVSYLNLLRATRAQKLDARPPSDLPTSRLFE
                     GVGLAAMNTNLVDGKENVQIHFKSSPFGRQSHGYNSNNAFLLNVRGEPVFIRSGKRDL
                     YGSPHHREWMWETKSDNAILINGTGQITRLPSASGQITHWATSKTVDVVAGEAAQSYP
                     DTLTRWGRRIIFLKPNVIVIHDVLDATAPSTFQWMLHAPHHSFEIGDNQVLWSGEPGT
                     VRVRFLEPAGLRISQSDKFDTPLADWVTWKQEQYHLTADAFEP"
BASE COUNT          415 a          507 c          491 g          414 t
ORIGIN      
        1 tggctcaaaa gcatcagcag tcagatgata ctgctcctgt ttccaggtaa cccagtcggc
       61 aagcggtgta tcaaacttgt cagactggga aatccgaaga cccgccggct caaggaaccg
      121 aactcgaacg gtgcctggct caccagacca aagcacctga ttgtccccga tctcaaagga
      181 gtgatggggc gcatgaagca tccattgaaa cgtggaagga gcggtcgcgt ccagcacatc
      241 gtggatgaca atgacattcg gcttgagaaa aatgatccgt cgtccccatc gagtcagtgt
      301 atcgggatag gactgcgccg cctcccctgc cacaacgtcc actgtttttg acgtcgccca
      361 atgggtaatc tgccccgacg cactgggcaa gcgagtgatc tgtcccgtcc cgttgatgag
      421 aatggcattg tcggatttcg tctcccacat ccattctctg tggtgcgggc tgccgtagag
      481 atcgcgcttt ccgctacgga tgaagaccgg ttctccgcga acattgagca gaaaggcatt
      541 gttggaattg tagccatgac tctgccgtcc aaagggactg ctcttgaaat gaatctggac
      601 gttttccttc ccatcaacca ggttggtgtt catagccgcc aaaccaacgc cctcaaacag
      661 tcgagaagtg ggaagatcgc tgggcggcct ggcgtcaagt ttctgcgctc gcgtcgcccg
      721 aagcagattc aggtaactga cacttggccg atgcccttga gactccgcgt accacaacca
      781 gtaaggattt gaggcaccct gagccagttc catcaccaca ggtgcaaggc ttttggagtt
      841 gatcagaatc gcttggtcgg caaatccgcc agtccctgtt ccaggtggca ccacataaag
      901 gggatagtac cccgcttgat ggtaaaaggg gcgatcgtat atgttgatct tcagaatgga
      961 ctgcatcaca tctgcccagt agaagaatcg agacatgtag ctcgaccagt aggccgtacc
     1021 ttcgtgccat ccaccctgcg agtcccccca aactggatag gctgtgtaga aaatggtcat
     1081 ggcgtagtcg agccagacag aggcttcggg gatgtcgtgg tagaacgcca tcgccagttc
     1141 tcccatcttg tgccatgctc ggttgctgtg gctggcatag ggacgccagt gatgccgatt
     1201 accggtcaga tactcaaaga catgccgacc tcttatgttc atgacttcga tcacgcgcga
     1261 gcgttcctgt tctgtaagaa agtctccaag aaaggtgtac gccctcgcag gcagatagag
     1321 cgcgggcatt cctgcttcgt cattgtattg gtagttggta gcaccgtttg gatcccattc
     1381 acagaaggcc ataagcagct cccgcgctgc ccgcgcgtat ttctcgtcac cagcaattcg
     1441 gtaagcaaac gccaatgtcg aagcagcatt tcccacagcc acagttcggg ttcggttgcc
     1501 ccaccagata ttcttccaga cttcgctcag cctgactgtt ccttcggggt acagaggagg
     1561 ctccgtcgag tcggggggct tttcaatgag tttgtctgcg gacgcaacta actctgtcca
     1621 gatttccttg agatcgcctt cacaacgtgc cttgacttgg gcaacatcct cgggacgaag
     1681 gaacagcctc ggatgatcca atgggatcat ctccttcaac atttcagaag tcggttgagg
     1741 aaagacggcg gccttcctgg tcaaagtgaa cgatcgaacg gtgctccaac cagtgagctg
     1801 agtggtggag ccttccttct cgccagc
//
LOCUS       DAOQUC010000907         1827 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_83802_length_1827_cov_2.814334, whole genome shotgun sequence.
ACCESSION   DAOQUC010000907 DAOQUC010000000
VERSION     DAOQUC010000907.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1827
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83802_length_1827_cov_2.814334"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..206
                     /locus_tag="PKH07_13900"
     CDS             <1..206
                     /locus_tag="PKH07_13900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36076.1"
                     /translation="RFVSADAQATPVLDCERNRLLWSQEPTGCLAGRDVTKADLHHGL
                     SICGQILNPLNRPTGSAYSQRDT"
     gene            208..495
                     /gene="gatC"
                     /locus_tag="PKH07_13905"
     CDS             208..495
                     /gene="gatC"
                     /locus_tag="PKH07_13905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HNT36077.1"
                     /translation="MSISLDQVRHIALLARLEFGDTELEKFASQLNSILQYAEKINEL
                     DTESVMPTAHAIPMANVFREDEVKPSLPVEQALQNAPVHNKATFIVPKVAE"
     gene            552..1265
                     /gene="pgeF"
                     /locus_tag="PKH07_13910"
     CDS             552..1265
                     /gene="pgeF"
                     /locus_tag="PKH07_13910"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HNT36078.1"
                     /translation="MHHGVSGRPIGCPEDCVEANMSFDWGDQTVATHYRSDFAERLGV
                     ETSSIVLPRQVHGSDVLVVARRGHDRPFLGQADGLVTAQWDVALGILVADCIPIFLLD
                     RATPAIGLVHAGWRGTVAGIAAKALGVMQDECDTKPENCIVWIGPSIGVCCFEVGPEV
                     TAEFQNAFPRNGIEFGDHIDLKQVNRQILLSKGVPDDQIEISPECSYCSDGFFSHRRA
                     VHRGLERTGRMMGLLALRR"
     gene            1469..>1827
                     /locus_tag="PKH07_13915"
     CDS             1469..>1827
                     /locus_tag="PKH07_13915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020250101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNT36079.1"
                     /translation="MAKKVQKEIGWGLVGYGGAFNMGKAHGASIQKQKGMKVVAACDL
                     DAGRLPAAEQDFPGIKTYTSMDEMLKNPEVSGAVIITPHNTHADLAVKCLKAGKHVVV
                     EKPMCITVDEATAMINAA"
BASE COUNT          414 a          451 c          525 g          437 t
ORIGIN      
        1 gcaggttcgt atctgcggac gcgcaggcaa caccagtgct cgattgtgaa agaaaccgtc
       61 tcctttggag ccaggagcca acaggctgcc ttgcaggacg cgatgtcaca aaggccgatt
      121 tgcatcacgg actttctatc tgtggacaga ttctgaatcc tctgaaccgt ccgacgggtt
      181 ctgcatacag tcagagagac acatgagatg agtatcagtc tggatcaggt gagacacatt
      241 gcccttctgg cgcgactgga gttcggtgat actgaacttg agaagtttgc cagtcagctg
      301 aattcgatcc ttcagtacgc agagaagatc aatgaactgg atacggaaag tgttatgccc
      361 acggctcacg ccatcccgat ggcgaatgtc tttcgggaag acgaggtgaa gccctctctt
      421 cctgtcgaac aggcgctgca aaatgctccg gtccacaaca aggccacatt catcgtcccc
      481 aaagtcgccg aatgattgct ttacgtccgt tccccgtctt ttcctcgccc ctcctgcagg
      541 agtccggtct tgtgcatcat ggagtctccg gacgtccgat cggctgtccg gaagactgtg
      601 tcgaggcgaa tatgagtttc gactggggcg atcagactgt tgctactcat tatcgctctg
      661 attttgccga acgattgggt gttgaaacat ccagcattgt ccttccgcga caggttcacg
      721 gctcagatgt gttggttgtc gcacgacggg gccatgaccg accctttctt ggacaagcag
      781 atggactggt cactgcgcag tgggatgttg cgcttgggat tctcgttgcg gactgcattc
      841 cgatctttct gctcgacaga gcgacgcccg caattggtct tgttcatgcg ggttggcgag
      901 gtacggttgc aggtattgca gcaaaggcct tgggtgtgat gcaggacgaa tgcgacacaa
      961 agcccgaaaa ctgtattgtc tggattggtc ccagcatagg tgtgtgttgc tttgaggttg
     1021 ggcctgaagt gactgcagaa ttccaaaatg ctttcccacg caatgggatt gaatttggag
     1081 atcacattga tctcaaacaa gtcaacaggc aaatccttct ctcgaaaggc gttcccgacg
     1141 atcagattga gatcagcccc gaatgcagct attgcagcga cggattcttc tcccatcgtc
     1201 gagcggttca ccgcggtctc gaacgaactg gccgcatgat ggggcttctg gcgctgagac
     1261 gatagatctg agattgactg gttttatcag ccgttcatgt cttgtgatgg ctcagggcat
     1321 ttctttctgg caaacggaca ctctcctgat ccggcgtgcg tgggctcatc cggtagcgac
     1381 aggccgtgtg cagttgtcag gtcgaagcgg ctttggtatt gttcccgacg ttccttaaat
     1441 atacgttaga cagcaggaga gggtcgtcat ggcaaagaaa gtgcagaagg agatcggctg
     1501 gggcttggtt ggctatggtg gtgcgttcaa tatgggaaag gcgcacggtg catccattca
     1561 aaagcagaag ggcatgaagg tagttgcagc gtgtgacctt gacgcaggac gtctgccggc
     1621 cgcggagcag gattttccgg gcatcaagac ttatacatcc atggatgaga tgctgaaaaa
     1681 cccggaagta agcggtgctg tgatcatcac cccacacaac acccacgcgg atcttgctgt
     1741 caagtgcctg aaggcaggga agcatgttgt agtcgaaaag ccaatgtgca tcaccgtaga
     1801 tgaagcaaca gccatgatca atgcggc
//
LOCUS       DAOQUC010000908         1826 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_83888_length_1826_cov_3.324675, whole genome shotgun sequence.
ACCESSION   DAOQUC010000908 DAOQUC010000000
VERSION     DAOQUC010000908.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1826
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83888_length_1826_cov_3.324675"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1684)
                     /locus_tag="PKH07_13920"
     CDS             complement(<1..1684)
                     /locus_tag="PKH07_13920"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HNT36080.1"
                     /translation="MTDKKRKKGIIHLVCNAHLDPIWLWEWEEGAAEAISTFRTAADF
                     CDEYNGFVFNHNEVVLYKWVAEYEPTLFKRIQKLVKQGKWHIIGGWYVQPDCNMPSGE
                     SFARQGLVGKRYFKKHFGVDVKTAINFDPFGHTRGLAQILKKSGFDAYLFCRPGQNDC
                     PLPDDDFVWAGYDGTEILAHRSSLFYGNNLGGARSKVEAWLKQNPEKPIGMVLWGVGD
                     HGGGASRLDLAQLNALIDETPDWEIRHSNPDVYFAELAKKQHLLPRHGKDINPWAVGC
                     YTSIIRIKQKHRLLENELYAVEKMAANAWMQGLMEYPQDDLNQALEDLLLCEFHDVLP
                     GSCIQPAEEAAIRILDHGLEILARLKARAFFALSSGQPKPPDGQIPVFVYNPHPYKVK
                     QMVECEFQLADQNWADSFTQITVHQKGKTIPSQVEREVSNIPLDWRKRVAFHAELEPS
                     QMNRFDCILQVVPKKPETALRIRNGKVHFRTDELDVLINAKTGLMDRFRVNGTDFLRK
                     NAFQPLVIKDDVDPWGMCVKGYRNVEGAFTLMDAKEGAWFSGLERLKGIFDHE"
BASE COUNT          429 a          492 c          450 g          455 t
ORIGIN      
        1 tctcgtgatc aaaaatgcct ttcagcctct caagaccaga gaaccacgcg ccttctttcg
       61 catccatgag cgtgaacgca ccttcaacat tccgatatcc cttgacgcac attccccacg
      121 gatccacatc atccttgatc acgagaggct gaaaggcatt tttgcgaagg aaatcagtgc
      181 cgttgactcg aaaacgatcc atcagccctg tctttgcgtt gatgagcaca tccagctcgt
      241 cggtcctaaa atgaaccttg ccatttcgaa tcctgagagc agtctcgggt ttctttggaa
      301 ccacctgaag gatgcaatcg aaccggttca tttgacttgg ttccagctca gcgtgaaatg
      361 cgacacgttt tcgccagtca agtggaatgt tgctgacttc acgttctacc tgagagggga
      421 tcgtcttgcc cttttgatga accgtgatct gagtgaatga gtctgcccag ttctgatcgg
      481 caagctgaaa ctcacattcc accatctgct ttaccttgta gggatgcggg ttgtaaacga
      541 agaccggaat ttgtccatcg ggaggtttgg gttgtccaga tgacagagcg aagaaggcgc
      601 gcgccttgag ccgcgccaaa atctccagac cgtgatcgag gattcggatc gccgcctctt
      661 ctgccggttg aatgcaggaa ccaggaagca catcgtgaaa ttcacacagc agtaagtcct
      721 cgagcgcctg gttcagatcg tcctgtgggt actccatcaa cccttgcatc caggcgttgg
      781 cagccatctt ctcaacggcg taaagttcat tctccaagag acgatgcttc tgtttgatgc
      841 gaatgatgct ggtgtaacag ccaactgccc acgggttgat atccttccca tggcgcggca
      901 gaagatgctg cttcttggcg agttccgcaa agtagacgtc gggattggaa tgacggatct
      961 cccaatccgg cgtctcgtct atcagggcgt tcaactgcgc cagatcaaga cgtgatgcac
     1021 cgccgccgtg atcaccaact ccccagagca ccatgccgat gggtttctct ggattctgct
     1081 tcagccaggc ttctaccttg cttcgcgccc ctccgagatt gttgccataa aaaagtgaag
     1141 aacggtgtgc aagaatttcc gtaccgtcgt accccgccca gacgaagtcg tcgtcgggga
     1201 gtggacagtc gttctggcct ggtcggcaga aaaggtaggc gtcgaagcca ctcttcttga
     1261 gaatctgagc cagcccacga gtatgaccaa acgggtcaaa gttgatcgct gtcttcacat
     1321 ctaccccgaa gtgtttcttg aaataacgct ttccaacaag cccctgacgg gcaaatgatt
     1381 ctccggatgg catgttgcag tccggttgaa cgtaccaacc accgatgatg tgccatttgc
     1441 cctgctttac gagcttttga attcgtttga acagcgttgg ctcatattcg gccacccatt
     1501 tgtagagaac gacttcattg tggttgaaga cgaacccgtt gtactcgtca cagaagtcag
     1561 ccgccgtgcg aaacgtcgag atcgcttctg cagcaccttc ctcccattcc cataaccaga
     1621 tgggatccag atgcgcgtta cagaccaggt ggatgatgcc ttttttgcgt ttcttatcag
     1681 tcactgaatt ttcctcaaga accagattgt cattgttgca catcaaatct gatgaagcag
     1741 cggattgttt atcatatccg acgtcagagt gctatgggag actccagaat agggattttg
     1801 cgatagacac agtaaagtgc caacca
//
LOCUS       DAOQUC010000909         1825 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_84004_length_1825_cov_2.184181, whole genome shotgun sequence.
ACCESSION   DAOQUC010000909 DAOQUC010000000
VERSION     DAOQUC010000909.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1825)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1825)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1825
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84004_length_1825_cov_2.184181"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..394
                     /locus_tag="PKH07_13925"
     CDS             <1..394
                     /locus_tag="PKH07_13925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013361793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36081.1"
                     /translation="LMKMIPGMSQMQQMQNVQFDDRELVTVEAIINSMTPNERSNPKI
                     IGDSRRKRIAMGSGASPMDVTKLLKQFEKSRRQIKQMMSQMGVGIGGKLAQFGSKVTH
                     VAGNVLRQKEKAKLKNRAKRKREKKRRK"
     gene            418..1191
                     /locus_tag="PKH07_13930"
     CDS             418..1191
                     /locus_tag="PKH07_13930"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HNT36082.1"
                     /translation="MFHRQLVFAIVVLSFAMTSISVAQALVDDATGQIVLPVDEESPI
                     VAPEAPASETEITSSEDAVESEPAAAVQPESATSEQPQEKQPEPVAAQETESLPYAIP
                     RLALNEVIPGFVLPDVLGDSVRVPEDVAAQYVVILFFRGAWDPYAQRQLQSISGKYQT
                     LRGLNAFVVAISADSPDALATLRKANLYPFPILHDVDSVVGRAFKVYRPEMREDMPAC
                     FILNSEDRLVFFQKADSGPESLKVEQLVSVLTDLARQTR"
     gene            1278..1526
                     /gene="rpsP"
                     /locus_tag="PKH07_13935"
                     /pseudo
     CDS             1278..1526
                     /gene="rpsP"
                     /locus_tag="PKH07_13935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011230697.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
BASE COUNT          473 a          430 c          505 g          417 t
ORIGIN      
        1 cctgatgaag atgatcccgg gcatgagtca gatgcagcag atgcaaaatg tccagttcga
       61 tgatcgagaa cttgtgactg ttgaggcgat catcaactcg atgactccca acgagcggag
      121 taatccgaaa atcatcggtg acagccgtcg gaagagaatc gccatgggga gtggcgcatc
      181 tccgatggat gttactaaac tactgaagca atttgagaag agccgacgac agatcaagca
      241 gatgatgtca cagatgggtg ttggtatcgg tgggaaactt gcgcagtttg gcagcaaggt
      301 gacccatgtt gccggcaatg tactccgtca gaaggaaaaa gcaaaactta agaaccgagc
      361 caagagaaag cgtgagaaga agcgcaggaa gtaataagca tccaggagga aaggattatg
      421 tttcacagac agttggtttt cgcgatcgtg gttctgtcct ttgctatgac aagcatctcc
      481 gttgcccagg ccttggtgga tgatgcgacc ggacaaattg tgcttcctgt tgatgaggag
      541 tcgccgatag tggctcctga ggcaccggca tccgagacag agattacttc ctcggaagat
      601 gctgtggaat cggaaccggc tgctgcagtg cagccagagt cagcaacatc cgaacagccg
      661 caagaaaagc agcccgaacc agttgctgca caggaaaccg agagccttcc ctatgcaatc
      721 ccccgacttg ccctgaatga ggtcatccct ggatttgtac ttcctgatgt tcttggggac
      781 agtgttcgag ttcctgaaga tgtggccgct cagtatgtcg ttatcttgtt ttttcgaggt
      841 gcctgggatc cctacgcgca gagacaactt cagagcattt cgggaaagta ccagacgttg
      901 aggggtctga atgctttcgt tgtggctatt tctgcagaca gtccggacgc actagctaca
      961 ctgcgcaagg ccaaccttta tccgtttccg attctccatg atgtggacag tgttgtcggc
     1021 agagcattca aagtctaccg acccgagatg cgtgaggata tgcccgcatg cttcatcctc
     1081 aacagtgaag atcggctggt cttcttccaa aaggccgatt cgggtcccga atcgctgaaa
     1141 gttgaacaac tggtatcggt attgacagac ctggctcgtc aaacgaggtg actggagact
     1201 ttttgtgcga ggaaaatctc ctgctgtgtc gggagaacca atagagaatc aagcataaat
     1261 ctaaggaggc taatggaatg gcggtaaaga tccgctgcaa gcgaatgggt aacaggaatc
     1321 gtcccttttt cagattggtg gttgcagact ctcgattcca aagggacggg cggtttctcg
     1381 atattctggg tcactatgat ccattgcagg aacccgctcg tgtggaaatc gacaaggaac
     1441 tcactctgaa atggctggga cgcggtgctc agatctccga gacggccaag agcttactca
     1501 agaagacggg catctaccaa gagtaccaac agtatctcgc cgaagccaag aagaagagga
     1561 agggagctgc tgggacaacg gaggaggcat ctgtccaggc cgacgattct cagtcgtcag
     1621 attcggcttc acagcaagcc cctgacgcct gaatcgaaac gcgacagaac aagtttcaag
     1681 acaattgctt tgagaagtag cagttactgc aatcgcatcg gagactctct cagcgttctc
     1741 tcaggagccg catcacgtgt gctgtttgcc tgatgaacgt ctcatgcctg ttctattctt
     1801 gatacctttg tggatcaagt ctgat
//
LOCUS       DAOQUC010000910         1822 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_84207_length_1822_cov_3.098472, whole genome shotgun sequence.
ACCESSION   DAOQUC010000910 DAOQUC010000000
VERSION     DAOQUC010000910.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1822
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84207_length_1822_cov_3.098472"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..926
                     /gene="scpB"
                     /locus_tag="PKH07_13940"
     CDS             <1..926
                     /gene="scpB"
                     /locus_tag="PKH07_13940"
                     /inference="COORDINATES: protein motif:HMM:NF016005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HNT36083.1"
                     /translation="EVEDSPAVGEPDLHNDLDGTDEADDEFEQSPVAYSMDVEDADDI
                     SSDSSSEEVEIETAPCDEENSPLLDPDQLDRVLLSLLFVADNSVPVQRFLPVFETEAN
                     LIAPKNERERKELELQLIDEIVAGLERIAARFETDESSPLQLVEIAGGYQLCTKPEMS
                     VHIDRFFERRKRATLSGAALETLAIIAYQQPITRAELEAIRGVNVDYIVHSLLERRLI
                     RVAGRKDAPGKPFMYRTTRFFLEYFGLGSLDDLPQVDELREPFSREPSEQQEGLEEDA
                     ESPVEVPSEAASSETEALPDSQDHDLSEPTP"
     gene            984..1730
                     /locus_tag="PKH07_13945"
     CDS             984..1730
                     /locus_tag="PKH07_13945"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009225844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNT36084.1"
                     /translation="MMETSKSERLQKILASAGIGSRRYCERLIVEGRVTVDGKQIDSL
                     GTKADPEHSKITVDGKPLGTMEVCLTLMLNKPVGVVTTLSDPQGRPSIKDCIPKEYAA
                     LRLYPVGRLDMDTSGLLLLTNDGDLAYRVMHPKHEVTKWYQAEVTGTLKEQTLQRLRT
                     GVLLDDGLTAPAEVQVQGQRENRTVVLIGIREGRNRQVRRMFAAVKCRVVFLKRLAIG
                     PLKLGDLGKGECRPLSNQELDELTRVLQED"
BASE COUNT          433 a          443 c          526 g          420 t
ORIGIN      
        1 aggaggtcga ggattctcct gcagttgggg aaccggattt acacaacgat ctcgatggca
       61 ccgatgaggc ggatgatgag ttcgagcaat ctccggttgc gtattctatg gatgtcgaag
      121 atgccgatga tatttcgtcc gacagttcat ctgaagaagt tgagatagaa actgcgcctt
      181 gtgacgagga aaactcaccc ttgcttgatc ccgatcaact ggatcgggtt cttctgtctc
      241 tgcttttcgt tgcggacaat tccgttcctg ttcaacgttt tcttccagtc tttgagaccg
      301 aagcgaatct cattgcaccc aagaacgaac gagagcgcaa agagctggaa ctgcagctga
      361 ttgacgagat cgtggcagga cttgaacgaa ttgccgctcg ttttgaaacg gatgagagca
      421 gtcctctcca gttggtggag atcgctggcg gatatcagtt gtgcaccaag ccggaaatgt
      481 ccgtgcatat tgaccgtttc ttcgaacggc gcaaacgtgc aacactctcg ggcgcggctt
      541 tggaaactct ggcgatcatc gcctaccagc agcccatcac ccgtgcggag ttggaagcga
      601 ttcgaggagt caatgtggat tacatcgtcc actcacttct ggagcgccga ctgattcgag
      661 tcgcaggacg caaggatgcg cctggcaagc ctttcatgta ccgaacgact cggttcttct
      721 tggaatactt tggattgggg agtttggatg atctaccgca ggtggatgag cttcgagagc
      781 ccttctcccg agaacccagc gaacaacaag agggtcttga ggaagacgct gaatctccag
      841 ttgaggtgcc ctctgaggca gcatcttctg aaacggaggc tctgcctgat tcccaggatc
      901 acgatctctc agaacccaca ccgtaggttg gtttgtgtct gacgcaccgt ctggatgcga
      961 ccagctcacg ctttggattg cagatgatgg aaacatccaa atctgaacgg cttcagaaga
     1021 ttctcgccag cgccggaatc ggctcacgtc gctactgtga gcgcctgatt gtcgaggggc
     1081 gtgtcacggt ggatggaaag cagatagact ctcttggaac caaggcagat ccggaacact
     1141 cgaagatcac agttgatgga aagcccctgg gaacaatgga agtatgtctg accctgatgc
     1201 tcaacaaacc ggtcggcgtc gtgacgactc tgagtgatcc ccagggaaga cccagcatca
     1261 aggactgtat ccccaaagag tatgcggctc ttcgcctcta tccagtcgga cgcttggaca
     1321 tggacaccag cggactgctt ctgttgacca atgacgggga tttggcctat cgagtcatgc
     1381 atccgaaaca tgaagtgacc aaatggtacc aggcagaggt gacgggcact ctcaaggagc
     1441 agacccttca gcgactgcgt acgggagtgc tgctcgatga cgggctgacc gcacctgccg
     1501 aagttcaggt tcagggtcaa cgtgagaacc gaaccgttgt cttgatcggc attcgcgaag
     1561 gaagaaaccg acaagtgcgg cgaatgtttg cagccgtcaa gtgtcgtgtt gtattcctga
     1621 agaggcttgc cataggccca ctgaagttgg gtgatttggg aaaaggggag tgtcgccctc
     1681 tgtcgaacca ggaactcgat gagttgacga gggttcttca ggaagactga aggaggagca
     1741 cgttttgaaa gaacgatttc tctatggtgg tgctctggta gatatgtttc tttttcattc
     1801 tgagaggaat gcagcaggaa gg
//
LOCUS       DAOQUC010000911         1821 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_84296_length_1821_cov_2.910532, whole genome shotgun sequence.
ACCESSION   DAOQUC010000911 DAOQUC010000000
VERSION     DAOQUC010000911.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1821)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1821)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1821
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84296_length_1821_cov_2.910532"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..647
                     /locus_tag="PKH07_13950"
     CDS             <1..647
                     /locus_tag="PKH07_13950"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNT36085.1"
                     /translation="VVLIFDDAYEGYVYDDDRLQRSPFIDFVNQDPRILPIKLDGISK
                     EILFYGGRLGFMTFGIPKQWVEKSEMTAIQADLENKISGLVRSSVSNCPRVVQSLTLM
                     ALENQDALVAQRQRVINVLTKRVLAFREALANVTIPNLETDPFQAGFFAFMNLKNIVP
                     ADLAEVLMSRYATGVVPLDKGTMKGIRVAFCSVPEKDIPALVSNIAKACADLSK"
     gene            1040..>1821
                     /locus_tag="PKH07_13955"
     CDS             1040..>1821
                     /locus_tag="PKH07_13955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008846267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HNT36086.1"
                     /translation="MERPDQKTPSYLHGFEQEYEQSPRQAAIDWFRNARYGLFLHYGL
                     YSLLGRHEWVQYREKIPVEHYAKLMDSFTAASFDARAIVRFAKECGMCYVNLTTRHHD
                     SFCLFRTSQTDFNSLNSPARRDLVEELAEACREESLGLFLYYSHGRDWRHLHAPNNDQ
                     WKGAARPEYDPPEPRYATGQDHNLNHYLDFVSAQITELLTQYGAIAGIWLDGIGVPLS
                     GDFSQFRCQELYDLIHRLQPQALVSYKQGLLGTEDFFAPEHKA"
BASE COUNT          441 a          493 c          465 g          422 t
ORIGIN      
        1 aagttgttct catctttgac gatgcgtacg agggctatgt ctatgatgac gatcggctcc
       61 agcgttcccc tttcattgat tttgtgaacc aggatccgag aatccttccc atcaaactcg
      121 atggaatttc caaggaaatc ctgttctacg gtgggcgact tggtttcatg actttcggca
      181 ttccgaaaca atgggtcgaa aagtctgaaa tgacggccat tcaggcggac ttggagaaca
      241 agatcagcgg gctggttcgc agttccgttt ccaactgtcc cagagtggtt cagagcctca
      301 ccctgatggc actggagaat caggatgcgc tggtcgcaca gcggcagcgc gtcatcaacg
      361 ttctgaccaa acgggttctg gcgttccgag aggctctcgc caatgtcaca atccccaacc
      421 ttgagacaga tccgttccag gcgggctttt tcgccttcat gaatctcaag aacatcgttc
      481 cggcagactt ggctgaagta ctgatgagcc gatatgccac aggtgttgtg cctttggaca
      541 aaggaaccat gaaagggatt cgggtggcct tctgtagcgt gccggagaag gatatccccg
      601 ctctggtcag caatatcgcc aaagcgtgcg ccgacctgag caaatagtgg ctgccaggag
      661 accggacttc taagaagcca gttttcccag tcagcaagcg ttcgacgccc gcaggcgcag
      721 gcctgctctt ggtgacgctc agttcagaac aggtgtgtca tctatcgaac aagcctccga
      781 gggaatgggc atagcccaca cgcccgcagg cgcaggcctg ctcggaaggg atagtcacct
      841 ttcgggttct gtcgcatcgg gtgtcgtgga atcagtcttt cacacaagca tgagtgcgca
      901 ccctgatcca attccgtgat cgcagtgctt ttcctgttac actattgcca agataaccaa
      961 atggccagaa acacagatgg gatcggcgac caacaatctg tatgatctgt cagacctaaa
     1021 tcaacaatgg agagcaacca tggaacgtcc cgaccagaag acaccttctt accttcacgg
     1081 atttgagcag gaatacgagc agagccctcg acaagcagcg atcgactggt ttcgaaacgc
     1141 tagatatgga ctcttcttac actacggtct ttattcgctc ttaggtcggc atgaatgggt
     1201 acagtatcgg gaaaagatcc ccgttgagca ctatgccaaa ctgatggaca gttttactgc
     1261 tgcctccttt gacgccagag cgattgtccg cttcgccaag gaatgcggaa tgtgctatgt
     1321 caacctgaca acaaggcatc acgattcctt ctgtctcttc agaacaagtc agacggattt
     1381 caacagtctg aactctccag cccgaaggga ccttgtcgag gaattggcgg aagcctgccg
     1441 cgaagaatcc ctcggtttgt ttctctacta ctcacacggt cgtgactggc ggcatctaca
     1501 cgctcccaac aacgatcaat ggaaaggcgc ggcacgaccg gaatacgatc cccccgagcc
     1561 tcgatacgcc acaggacaag atcacaacct gaaccactat ctggattttg tgtctgcaca
     1621 gatcacggaa ctcttgaccc aatacggtgc aatcgctggg atttggctgg atggaattgg
     1681 cgttccgctt tccggagatt tctctcagtt caggtgccag gaactctacg atctgattca
     1741 tcgtctgcaa ccgcaggcgc ttgtttccta caaacaggga ctgctcggaa cagaggattt
     1801 ctttgctcct gaacacaaag c
//
LOCUS       DAOQUC010000912         1820 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_84356_length_1820_cov_4.194901, whole genome shotgun sequence.
ACCESSION   DAOQUC010000912 DAOQUC010000000
VERSION     DAOQUC010000912.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1820
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84356_length_1820_cov_4.194901"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..329
                     /locus_tag="PKH07_13960"
     CDS             <1..329
                     /locus_tag="PKH07_13960"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HNT36087.1"
                     /translation="DRTKALQRAADDQDLLREIMKVFVEDAPRQLREIHEAFDSQDAE
                     EVRKKSHALRGAAGNVSAVALQMKAQEIEQQSLQASFDQIEPQIQSLDGLFEQFKQSL
                     IQHGDI"
     gene            383..1036
                     /locus_tag="PKH07_13965"
     CDS             383..1036
                     /locus_tag="PKH07_13965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020080282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HNT36088.1"
                     /translation="MTETLKNQYLIDDFTTQDTWRIFRIMSEFVEGFEDLASVGSCVT
                     IFGSARVKEEDPHYQLAREMAAKLAGKGYAIMTGGGPGIMEAANRGAAEAHGQSIGLN
                     IELPMEQNPNPYSNIRINFRYFFVRKVMLVKYSQAMLVFPGGFGTLDEFFETVTLIQT
                     HKVKKYPVVLVVRSFWSGLLDWIRIHLLDTGKISEEDLELFKMVDTVEEAMQAIGAQ"
     gene            1132..>1820
                     /locus_tag="PKH07_13970"
     CDS             1132..>1820
                     /locus_tag="PKH07_13970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36089.1"
                     /translation="MRKNSSVCLALFSLAVALVATAPVFAESLSAKQIIRQSAMAYQQ
                     METYQDKSMMQIDLGDTMASMPGNVSEAQKQQMLEMTKMQMDFGFKRPRQFAMNLKSF
                     MFNADTSCDGKTLTYLIANLMGQPLNQYQQRNVENASIDKVLDEMASSLAMQGQMKGQ
                     SHQSNVVYSMLLADNPLEAFSKMIDSVSPEAVREKKQIRMNADQSEFKDVYILQGNFA
                     VDQTYGKGITAV"
BASE COUNT          481 a          441 c          488 g          410 t
ORIGIN      
        1 atgaccgaac gaaggcactg caacgagcgg cggacgatca ggatctgctg cgagaaatca
       61 tgaaggtttt tgtggaagat gccccaaggc agttgcggga gattcatgag gcttttgaca
      121 gtcaggacgc cgaggaagtt cgaaagaagt cacacgctct gagaggcgcg gccggcaatg
      181 tttccgccgt tgcgcttcag atgaaagcgc aggaaatcga acaacagagt ctgcaggcct
      241 cttttgatca aatcgagccc cagatacagt cgttggatgg actctttgag caattcaaac
      301 agagcctcat ccagcacggt gatatatgag acaaggtgag tcagactaca ttgattcgaa
      361 ccattgaagg gagaacagga aagtgaccga aacgctcaag aaccagtatc tcattgacga
      421 tttcacgacg caggacactt ggcgcatatt ccgaatcatg tcagaatttg tcgagggttt
      481 cgaagatctg gcaagtgttg gctcttgtgt caccatcttc gggtcggcgc gcgtcaagga
      541 agaggatccg cactatcagc ttgcccggga aatggccgcg aaacttgccg ggaaggggta
      601 tgcgatcatg accggtggag gtcccggaat catggaagcg gccaatcgcg gtgccgctga
      661 agcccacggg cagtccatcg gtttgaacat cgaactcccc atggaacaga accccaaccc
      721 gtactcgaat atccgaatca actttcggta tttctttgtc cgtaaggtga tgctggtcaa
      781 gtacagccag gccatgttgg tctttccagg cggttttgga acgctggatg agtttttcga
      841 aacggtcacg ctgattcaga cgcacaaagt caaaaagtac cctgttgtgt tggtggtcag
      901 gagtttctgg tctggtttgc ttgactggat tcgcattcat ctgctggaca cgggcaaaat
      961 atctgaagag gatctcgaac tgttcaagat ggtggacacc gttgaggaag ccatgcaagc
     1021 catcggggct caataattgt ggaaaagaac ttcagatttg aataacgaac cctttccaga
     1081 gtatgatcca gaagtaggga agagcatgac acaatccagg aggtcttgac cgtgcgtaaa
     1141 aactcgtcag tttgtctcgc tttgttctct cttgctgttg ccctcgtcgc gacagccccc
     1201 gtgtttgccg agagcctttc tgccaaacag atcatccgac agtccgccat ggcataccag
     1261 cagatggaga cttaccagga caagtccatg atgcaaattg accttggcga cacgatggcc
     1321 tccatgcccg gcaatgtctc agaagcccag aagcaacaga tgcttgagat gaccaagatg
     1381 cagatggatt tcggcttcaa gagacccagg cagtttgcaa tgaacctgaa atcgttcatg
     1441 tttaacgcag acacatcctg cgacggcaag actttgacct atctcatcgc gaatcttatg
     1501 ggacaacccc tgaatcagta ccagcagagg aatgtcgaga atgcgagtat agacaaagtc
     1561 cttgacgaga tggcttcttc tctcgccatg cagggacaga tgaagggaca gagtcaccag
     1621 tcgaatgttg tctactcgat gcttctggcg gacaatcccc ttgaagcctt ctccaaaatg
     1681 atcgattcgg tcagtccgga agccgtccgt gagaagaagc agatccgaat gaacgccgat
     1741 cagtccgagt tcaaagacgt ttacatcctg cagggcaact ttgctgtcga tcagacttat
     1801 ggcaaaggta ttaccgcagt
//
LOCUS       DAOQUC010000913         1817 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_84569_length_1817_cov_3.979569, whole genome shotgun sequence.
ACCESSION   DAOQUC010000913 DAOQUC010000000
VERSION     DAOQUC010000913.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1817
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84569_length_1817_cov_3.979569"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..530
                     /locus_tag="PKH07_13975"
     CDS             <1..530
                     /locus_tag="PKH07_13975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36090.1"
                     /translation="LSDFLCNRHKLDFLPPQADPDIRPETLQLLGRSGQKDFQERLKT
                     TIQPELERARQYFEAFATVVKEEEEVRPKEQAISEEQVEQAPEKQPEVVVFESQPKSA
                     VPMWTLLIPGLYQILRGEIVPGALILMIFLVGAGFAFLFSLMGRLGPAFWSAGMGMLA
                     WFSGIFLAWSGKDLS"
     gene            complement(546..1316)
                     /locus_tag="PKH07_13980"
     CDS             complement(546..1316)
                     /locus_tag="PKH07_13980"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HNT36091.1"
                     /translation="MRILTCNVRVSSAKDGENGWEFRRALCAEVILRQQADLICFQEM
                     HSDQFNYLADLLPEFAPFGMSDHPLGDHPLNAIFHRKDSFTVLENAGYWLSETPHISG
                     TKSWDSKCIRFANWIRLKDVSTGRDFRIVNTHLDHVSQPARENQARLLVEDASAYPAD
                     YPQFLTGDMNCDATNSAFAIFKEGGWQDTYSAVHGVENPGTTYHAFKGSECSWPVGKM
                     DFIFCRGSVRVVASKIVDDSREGRYPSDHYFISADVDL"
     gene            complement(1357..>1817)
                     /locus_tag="PKH07_13985"
     CDS             complement(1357..>1817)
                     /locus_tag="PKH07_13985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36092.1"
                     /translation="ELQAGGFAFQTEGQCKARNLEFPIDVSLVGAQVAPKQTSNIPVL
                     GQLKAGSMPVLIEALKDKDGNIVYQGHIKGTLDKPEFFSWKSMGAFLASSLVKNAQKL
                     PDVQGVVDTSKRLLEGSRTDVEKATQGVLEQAGKTGESIRGILPFGKKKD"
BASE COUNT          428 a          500 c          451 g          438 t
ORIGIN      
        1 agttgagcga cttcctctgc aacaggcaca aactggattt cctgccccca caagcagatc
       61 ccgacattcg accggaaaca cttcaactgc ttgggcgctc aggacagaaa gatttccagg
      121 aacgtctcaa gaccacaata cagccggagc tcgaaagagc tcgtcagtat ttcgaagcct
      181 ttgcgacagt tgtcaaagaa gaagaggaag tccgacccaa agagcaggcc atttcagaag
      241 aacaggttga acaggcaccc gaaaagcaac ccgaagtcgt cgttttcgaa agccaaccca
      301 agtcggccgt ccctatgtgg acattgctta tcccagggct ttaccagata ctgcgaggag
      361 agattgtccc tggggctttg atcctgatga tctttcttgt gggtgctggg tttgcattcc
      421 ttttcagttt gatgggacgc ctcggccccg ctttctggtc ggcgggcatg ggaatgctgg
      481 catggttctc tggaatcttc ctggcttggt cgggcaaaga tctcagttga tctcagccgc
      541 tccagtcaca gatcaacgtc cgcactaatg aagtagtgat cgctgggata tcggccctct
      601 ctcgaatcat caacgatctt ggaagcaacc acacgaaccg atccgcgaca gaagatgaaa
      661 tccatttttc cgacaggcca ggaacactcc gaacccttga acgcatgata ggtggtgcct
      721 ggattttcga caccatggac tgcggagtat gtgtcctgcc atccaccctc tttgaatatc
      781 gcaaaggcac tgttggttgc atcgcagttc atgtcgccgg tcaagaactg gggatagtca
      841 gccgggtaag cggaagcgtc ttccaccaga aggcgtgcct gattctcccg agcgggctgg
      901 ctgacgtgat ccaggtgggt gttgacgatt cgaaaatccc tgcctgttga aacatccttc
      961 aagcgtatcc agttggcgaa acggatacac ttactgtccc aagacttggt tcctgaaatg
     1021 tggggggttt ccgaaagcca gtatcctgcg ttctccaaca cagtaaacga atccttccgg
     1081 tgaaaaatcg cgttcagcgg atgatcaccc aaagggtgat cggacatgcc gaagggagca
     1141 aactcaggca acaagtcggc aaggtaattg aactggtcgc tgtgcatctc ctgaaaacag
     1201 atcagatctg cctgttgccg caggatcact tctgcgcaaa gggctcttcg aaactcccaa
     1261 ccattctcgc catccttggc gcttgacact cggacattac aggtaagaat tctcatttgt
     1321 tgcggtctcc tcaacgtagt tcatcttgtg tgctcgtcag tccttcttct ttccaaaagg
     1381 cagaatccct cggattgatt ctcctgtctt gcccgcctgt tcgagcacgc cttgcgttgc
     1441 tttctcaacg tccgtcctcg aaccttccaa cagccgcttc gaagtatcca caacgccttg
     1501 gacatcgggc aatttctgcg catttttcac cagagatgaa gccaagaagg cgcccatgga
     1561 tttccagctg aagaactcgg gtttgtccaa agttcctttg atatgtcctt gatagacaat
     1621 gttgccatcc ttgtctttca gcgcttcgat cagcaccggc atgcttccgg ctttcagttg
     1681 acccaggaca ggaatgttcg acgtctgctt tggggcgact tgagcgccaa cgagcgatac
     1741 atcaatcggg aactccagat tcctggcctt gcattgtccc tcagtctgaa aggcaaaacc
     1801 accggcctgc agctcaa
//
LOCUS       DAOQUC010000914         1813 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_84915_length_1813_cov_2.684300, whole genome shotgun sequence.
ACCESSION   DAOQUC010000914 DAOQUC010000000
VERSION     DAOQUC010000914.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1813
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84915_length_1813_cov_2.684300"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            260..>1813
                     /locus_tag="PKH07_13990"
     CDS             260..>1813
                     /locus_tag="PKH07_13990"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HNT36093.1"
                     /translation="MDGNGVKKRLGDILVEQGVITRQQLERALREQSTTGKRIGDFLV
                     DMGFVRPEAILRALARQHGMKYVNLKETRLNPDAKNLLPESVVRDNKILPLEKRGDTI
                     RVAVADPDRIAILQEIAKQEGVQIEAYLSLDRDIDERVAALFPVFVQVETSDDFLDGV
                     EGIDSLTQIAETEDTLSVEELKAEAEDAPAILLVNKIVSHGITSAASDIHIEPKEKEL
                     QLRYRMDGVLKATKPIPKKLQNHILSRVKIISGMDISERRLPQDGRFRMQVGERVVDF
                     RVSSLPTIHGEKIVLRLLDKSRLPPGLSGIGLESREEKILLKVCTRPYGMILVTGPTG
                     SGKSTTLYSFLKELATPDRNVITIEDPVELENPLVNQVHVKEDIGLTFAQVLRTILRQ
                     DPDVVMVGEIRDLETVEIAIRTALTGHLVLSTLHTNDAPSTVTRLLDMGVAPFMISAA
                     LSVAIAQRLVRKVCDVCKVEYQPDEKVLDGLGLEGEERKGPFFRGKGCDQCNELGYRG
                     RIGIFEVIEM"
BASE COUNT          440 a          465 c          513 g          395 t
ORIGIN      
        1 aagtgtgtgc agtataacat ggtttgacaa aaccaagcca gagaactact ttatgcttgg
       61 ttcggtgtgg atgagaacat ctgaacgact gggacgttcg tttcacgcga tcggttaccc
      121 cgttgctgag tgttgcagga gcctgtcgcg gtttggtggt ctgctctcta ggaaaacact
      181 tcgaccagtg acaaaccgaa tactgtgtcc cgtgtctagg tgttgaagtg cagatgtgtc
      241 tggaggatat tgggatagca tggacggcaa cggtgtcaag aaaagattgg gcgacatcct
      301 ggtcgaacag ggtgttatca cccgtcagca gttggagcgc gccctgcgcg agcagtccac
      361 cacaggaaag cgcatcgggg attttctggt agatatggga tttgtccgtc ctgaggcaat
      421 ccttcgggct ttggctcggc agcacgggat gaagtacgtc aatctcaagg agacccgact
      481 gaatcccgat gccaagaatc tgctgcccga gagtgtggtt cgcgacaaca agattctccc
      541 gctcgagaag cgtggagata cgatacgagt tgccgttgcc gatccggaca ggatcgccat
      601 tcttcaggag attgccaaac aggaaggcgt tcagattgaa gcctatctga gtctcgatcg
      661 ggatattgat gagagagttg cggcgctgtt tcccgttttt gttcaagtgg aaacctctga
      721 tgattttctt gatggggttg aaggtattga tagtctcacg cagattgcgg agaccgaaga
      781 caccctcagt gtggaagagc tcaaagccga ggcggaagac gcgcccgcca tcctgctggt
      841 caacaaaatc gtctcacacg gcatcacctc agccgcctcg gatatccaca tcgaaccgaa
      901 agaaaaagaa ctgcagctgc gctaccgcat ggacggcgtt ctcaaagcca ccaaacccat
      961 accgaaaaaa ctccaaaacc atattctctc ccgagtcaag attatctccg gcatggacat
     1021 ttccgaacgc cgactccctc aagacggccg cttccggatg caggtggggg agagagtcgt
     1081 ggatttccgg gtttccagcc ttccaacgat ccacggagag aagatcgtgc ttcggctcct
     1141 ggacaagagt cgccttcctc ccggactgag tggaatcggc ctggaaagcc gggaagaaaa
     1201 gattctcctg aaagtctgta cacgacctta cgggatgatt ctggtgactg gtcctacagg
     1261 aagcggaaaa tccacaacgc tttattcctt tctcaaggaa ttggcaaccc ctgacaggaa
     1321 cgtcatcacc atcgaagacc ccgttgagtt ggagaaccct ctggtcaacc aggttcatgt
     1381 caaagaggat atcgggttga ctttcgccca ggtgcttcga acaattctgc gccaagaccc
     1441 ggacgtagtc atggtcggtg agattcgtga tctggaaaca gtggagatcg ccattcgaac
     1501 cgcgctcacc ggacacttgg tgctttcgac tctgcacacc aatgacgccc ccagcacggt
     1561 gacccgactt ctggatatgg gagtggcgcc tttcatgatc tcggcggcgc tgagcgtggc
     1621 cattgcacag cgcctggtgc gaaaagtatg cgatgtctgc aaggttgaat accaacccga
     1681 cgagaaagtc ctcgatggct tgggacttga gggcgaggaa cgcaaaggac ccttcttccg
     1741 cggcaagggc tgtgaccaat gcaacgaact ggggtaccga ggccgcatcg gcatctttga
     1801 agtcatcgag atg
//
LOCUS       DAOQUC010000915         1812 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_84981_length_1812_cov_3.096756, whole genome shotgun sequence.
ACCESSION   DAOQUC010000915 DAOQUC010000000
VERSION     DAOQUC010000915.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1812)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1812)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1812
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84981_length_1812_cov_3.096756"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(482..1147)
                     /locus_tag="PKH07_13995"
     CDS             complement(482..1147)
                     /locus_tag="PKH07_13995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011918915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic aminobenezylarsenical efflux permease
                     ArsG family transporter"
                     /protein_id="HNT36094.1"
                     /translation="MMELLLAVGTSFWLGILTSVSPCPLATNIAAISFVSRRIGNPSR
                     VFLSGLLYCFGRVVVYVVLGVVLVFSLLSAPYVSHLLQKYMSRFIGPVLILVGMLLLE
                     LLPVTFGGSGMSERMQKRVEMLGIWGAFLLGVLFALSFCPISAALFFGSLLPLALGVK
                     SAVIVPAVYGLGTGLPVFVFALLLAFGARRVGTVFDKIVHVELWARRLTGVVFILVGL
                     YYS"
     gene            complement(1144..1665)
                     /locus_tag="PKH07_14000"
     CDS             complement(1144..1665)
                     /locus_tag="PKH07_14000"
                     /inference="COORDINATES: protein motif:HMM:NF040494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrophenyl compound nitroreductase subunit ArsF
                     family protein"
                     /protein_id="HNT36095.1"
                     /translation="MNKKVLLRYFLLGIVVLSFAAVGVSEWREARSAKPDQLRQEGAS
                     VQELPGQDGVSGATQGHKVIAYYFHGNVRCTTCRKIEAFAKESVEKGFAAEIEAGLVE
                     WKTVNVEQAENEHFVQDYQLTTRSVVLSEVVDGTERRWKNLERVWQLVADQQLFSDYV
                     QEQLREYLKEPQA"
BASE COUNT          502 a          532 c          411 g          367 t
ORIGIN      
        1 ccaagcaatg cccgagtccg ttcaagattg tgagcatcca gccgagaacg ttcaacacca
       61 tagaaacgcc tctgtggctc cttgaaggca tagcctctga taaggagttc gatccagtcc
      121 agatcctcta ttgtggctct tctcaaagtg actgccattg ttcctctacc cacaagaata
      181 tgactgaagc attatgccca actgcgaaaa gatgtgcaat tggaaatcaa tatgccgatc
      241 atcaggggcg ttccctacgc cgtgacgacg gaatgcggct gagaccgacc ggactcttgg
      301 cgttcggagt atctttcccc gatgacatag agaccggagg ggcgtgtcat ttgttgagca
      361 ggcgtccaag agagtgggca tagcgcacag ccatcaggcg ctggcctgct cactatgaca
      421 tgcacatgcc cgcaagtgct ctcgttcaag gcactgttct agaaaatggc gagaatctgg
      481 gtcaggaata gtacagacca accaggatga aaacgactcc cgtgaggcgt cgtgcccaga
      541 gttccacatg cacaatcttg tcaaacacag tccccacccg tcgtgcacca aacgccagca
      601 gtaaagcaaa gacaaaaacc ggaaggcctg ttccaagacc atagaccgct ggcacgatga
      661 ccgcagactt cactccaagt gccaaaggca atagacttcc gaagaaaagc gctgcagaga
      721 tgggacagaa agagagcgca aacagaacac ccaacagaaa tgctccccaa atccccaaca
      781 tctccacacg tttctgcatc cgttcactca ttccggatcc accgaatgtc acagggagca
      841 gttccagcaa gagcattccc accagaatga ggacaggccc gatgaagcgg ctcatatact
      901 tctgtaacag atgagaaaca tagggtgccg agagcaaact gaagaccaac acaactccca
      961 ggacaacata gacgacaact cttccgaaac aatacagcag acccgaaaga aagactctcg
     1021 aaggattgcc gatccgccgg ctgacaaatg agatggcggc aatgttcgtc gccagtggac
     1081 aaggactcac cgaggtcaga atccccaacc agaaggaagt cccaaccgcg agcaggagtt
     1141 ccatcatgcc tgaggctcct tcagatactc ccgaagttgt tcttgaacgt agtcactgaa
     1201 aagctgttga tccgcgacca gctgccagac tcgctccaaa ttcttccatc gtctttcagt
     1261 tccatcaaca acttcagaca atacgaccga acgcgtcgtc aactggtagt cctgaacgaa
     1321 gtgttcattc tcggcctgtt caacgttcac agtcttccac tcgaccaaac ccgcctcaat
     1381 ctctgctgca aaacccttct cgacagattc cttggcaaaa gcctcgatct tgcgacacgt
     1441 tgtgcaacgc acattgccat gaaaataata ggcgatcacc ttgtgaccct gagtcgctcc
     1501 ggatacacca tcctgtcccg ggagttcctg cacagaggca ccttcctgtc tgagctgatc
     1561 gggctttgcc gaacgtgctt ctctccattc gctgacgcca acggcagcga agctaaggac
     1621 aacaatgccc aacagaaaat atcggagcag gactttcttg ttcatctcgc agattccctc
     1681 agccggcaag catctcaacg gctttgttgg ataccaccgc aatgctctca tcagtgacag
     1741 gagacttccc cttctccatt ccaatatcgg ccatctgaag atgctgaaac tttgaaattc
     1801 cggcctcttc aa
//
LOCUS       DAOQUC010000916         1812 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_85017_length_1812_cov_2.088788, whole genome shotgun sequence.
ACCESSION   DAOQUC010000916 DAOQUC010000000
VERSION     DAOQUC010000916.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1812)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1812)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1812
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85017_length_1812_cov_2.088788"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1295
                     /locus_tag="PKH07_14005"
     CDS             <1..1295
                     /locus_tag="PKH07_14005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36096.1"
                     /translation="PWCIAGFGDNPTIIDVLMVNPYQGVDFGTQAGGQRTGRHFIRNL
                     FGQPLRTGIYINGCLDIGRIENVHFWPFWMDDSYTSKYGEAFVFGKADWHYVFNTFTY
                     GYNVGYKFIQTSDGVCNGNFLGIGADGSHTAILVEQCSPLGLLITNGEFSSFSGDSPN
                     EVVTGPDFTGTIQFQNCAFWGSAKNIGFLQGNGAVSFQNCNFVRWSYHEAPQPALDVW
                     GGELSVVGCRFRTTGPQLHLREDVKQAVVTGNLSRAPELIVNESLGQVVLNSNVHAEF
                     PQEEKHAVVVDNAMMEGSCIQGAFEGGAGWKMYAGGGSYLGSTAWAPAGQGEFTATWK
                     PSLKRSGKYEVFVWYGDEKAEAKNATNAEFAVTHSERSWISWIQTTSKVTVDMSDNNR
                     RWVSLGTWRFQRNIAAQVQLTNEADGRVLADAVKFVRQ"
     gene            1442..>1812
                     /locus_tag="PKH07_14010"
     CDS             1442..>1812
                     /locus_tag="PKH07_14010"
                     /inference="COORDINATES: protein motif:HMM:NF024120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HNT36097.1"
                     /translation="MSINWSLFVLFGLLSLTAAHSVRAADCISVRDCGAIGDGVHDDT
                     DAFLKAVELARREQKNVYVPIGTYATSKTIVLDKVAMNGPQVSAWVGDESVHMPSIVP
                     THRDGPAFQMLDSSALDGIDI"
BASE COUNT          410 a          417 c          545 g          440 t
ORIGIN      
        1 acccgtggtg cattgccgga tttggcgaca atccgacgat cattgatgtt ctgatggtca
       61 atccatatca aggggttgat ttcggaacgc aggctggtgg tcagaggact ggtcggcatt
      121 tcatcaggaa tctttttggc caaccgctcc gaacgggaat ctacatcaac ggttgtctgg
      181 atatcggtcg catcgagaac gttcatttct ggcctttctg gatggatgac tcctacacaa
      241 gcaagtatgg agaggcattc gtctttggga aggcagattg gcattatgtc ttcaatacat
      301 tcacttacgg ttacaatgtc ggttacaagt tcatccagac atccgatgga gtgtgcaacg
      361 gcaactttct tggtatcggt gcggatggtt cgcacacggc gattcttgtg gagcagtgtt
      421 ctccgctggg tcttctcatc accaacggag agttctccag tttcagcggg gactcgccca
      481 atgaggtggt aaccggtccg gacttcaccg gtacgatcca gtttcagaat tgtgccttct
      541 ggggatcggc aaagaacata ggttttcttc agggtaacgg ggccgtcagt tttcagaact
      601 gcaattttgt tcggtggagt tatcatgaag cccctcaacc ggctctggat gtgtggggag
      661 gggaattgtc ggtcgttggg tgtcggttca gaacaaccgg accgcagctt catctccggg
      721 aggatgtcaa acaggccgtt gtgacgggca acctgtcaag agcaccggag ctgatcgtca
      781 atgagagtct tggccaagtg gtgctgaact ccaacgtcca tgccgagttc cctcaggagg
      841 agaaacacgc tgttgttgtg gacaacgcca tgatggaagg cagttgcatt cagggcgcat
      901 ttgaaggcgg tgcaggttgg aagatgtatg ccggtggcgg aagttatctc ggttccaccg
      961 catgggcacc agcggggcaa ggtgaattca ctgctacctg gaagccttcc ctgaagcggt
     1021 cagggaagta tgaagtcttt gtctggtacg gcgatgagaa ggctgaggcg aagaacgcga
     1081 ccaacgcgga attcgccgtg acccacagcg aacggtcgtg gatctcttgg atacagacaa
     1141 ccagcaaagt gactgttgac atgtcagaca acaacagacg atgggtctca cttggaacct
     1201 ggcgatttca gaggaacata gcggcgcaag tacagttgac caatgaagca gatggacggg
     1261 tactggcgga cgccgtcaag tttgtgcgcc aatgaagtgt tcttgccccg aaagtagctc
     1321 ttgggaaagc aggctctgaa gaagcatggt tcttcagtga gcaagtgtct gcaggagtgg
     1381 gtacagcccg tacgccggag gggctaaact gatcagagta tcggtcgtga ggagtttatc
     1441 aatgagcatc aactggtcct tgttcgttct ctttgggctt ctgagtctga cggcggcgca
     1501 cagtgttcgt gcggcagact gtatcagtgt tcgcgactgc ggtgccatcg gtgacggagt
     1561 tcatgatgat acagatgcct tcttgaaggc cgtcgaactg gcacgtcgcg agcagaagaa
     1621 cgtctatgtt cccattggga cgtatgccac atccaagacg attgtcctcg ataaggtcgc
     1681 aatgaatggt ccccaagtca gtgcctgggt tggcgatgaa agtgttcaca tgccttcgat
     1741 tgttccgacg catcgtgacg gacctgcttt tcagatgttg gattcctcag cgctggacgg
     1801 aatagatatc ag
//
LOCUS       DAOQUC010000917         1811 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_85070_length_1811_cov_2.759681, whole genome shotgun sequence.
ACCESSION   DAOQUC010000917 DAOQUC010000000
VERSION     DAOQUC010000917.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1811
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85070_length_1811_cov_2.759681"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1811
                     /locus_tag="PKH07_14015"
     CDS             <1..>1811
                     /locus_tag="PKH07_14015"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNT36098.1"
                     /translation="GIDGWRFCRLLRSAEYRAFNKVPILVVSATYSGEEASRISDDIG
                     ANAFMSAPVDGAHFVEQVRSLLGGRRAEELLRLLIVEDSKTQGNLLKKFMQSHGYTAD
                     IVQTASDAERAFFDQRYNVAVIDFHLPDRNGDQLLTAFREIQPECVFIMMTTDPSPEL
                     ALAWMRQGASAYIRKPFEPEYLLELLTKARRERALLRVEDLLEERTRELRASEAALRE
                     AEEIAQMGRWELDHHTGRLEWSDTIFSIFEIDKEHFGASYEAFLETIHPDDRDRVDLA
                     YRESLQHRKPYDVVHRLLMSDGRIKWLSERCSTDFDSEGKPIRSVGVVQDVSAVKKSE
                     QMLRERGDRLHRYARLLSDLIERGGFFTGSFKDAVKAATEAASSILKTERVSVWIYNS
                     DCSEIHCADLYQSSLKKHSEGENLKASDFPNYTEAHKIGQVIAAEDVFSDPRTSSIPR
                     AYFEDNRIKSLLDAPVWVQGKLAGLLSFEHTETVRTWLPEEEQLALTLASFVSLCLET
                     QERKKAEQALRISEKNLSSIFNVIKESVFLLDCEGIVLAANQTFAERLAVTVADCVGR
                     LVYSLIPSDIAEKRRAVVQRVLRTKEPIIFEDERRGL"
BASE COUNT          405 a          447 c          528 g          431 t
ORIGIN      
        1 tggcattgat ggctggcgct tctgtcgtct tttgcgctct gctgagtatc gtgcgttcaa
       61 caaggttccc atcctggtcg tctcggcgac gtattcggga gaagaagcct ctcgcatctc
      121 ggatgatatc ggggcgaatg cattcatgtc ggctccagtg gatggagcgc acttcgtgga
      181 acaagttcgc tcccttttgg gtgggcgtcg agccgaagag cttcttcgcc ttctcattgt
      241 cgaagacagc aaaacgcagg ggaatctgct caagaagttc atgcagtccc acgggtacac
      301 ggccgacatc gtccaaaccg cttccgacgc cgagcgggcg tttttcgatc agaggtacaa
      361 cgttgccgtg atcgatttcc atcttccgga tcgaaatggt gaccagcttt tgacggcatt
      421 tcgagagatc caaccggaat gcgtcttcat catgatgacg acggatccaa gtcccgagtt
      481 ggcgctggcc tggatgaggc aaggggcatc ggcctatatt cgcaagccct ttgagccgga
      541 gtacctactg gaactcctca ccaaggcgcg tcgtgagaga gcgttgctgc gggtcgaaga
      601 tctccttgag gaaaggacgc gcgaactgcg ggcgagcgag gccgctcttc gagaggctga
      661 ggagatcgcc cagatgggac gatgggaact ggatcatcat accggcagat tggaatggtc
      721 ggacacgata ttcagcatct ttgaaattga caaggagcac tttggcgctt cttacgaggc
      781 ttttctggag acgatccatc ctgacgacag ggacagggtt gacctggcct accgcgagtc
      841 tcttcagcac aggaaaccct atgatgtggt tcaccgactc ttgatgtctg atggccgaat
      901 caagtggttg agtgaacgct gttccactga ttttgattca gagggtaaac cgatccgatc
      961 ggtgggggtg gtgcaggatg tctcagcggt caagaagtct gagcagatgc ttcgggagcg
     1021 tggcgatagg ctgcacagat atgcacggct gctctcggat ctcatagagc ggggtggttt
     1081 cttcactggg agtttcaaag acgcagtcaa ggctgcaaca gaagccgctt caagtattct
     1141 taagaccgag agagtcagtg tctggatcta taactcagat tgttctgaga ttcactgtgc
     1201 cgatctctat caatcgagcc tgaagaagca ttcagaagga gagaatctca aggcatcgga
     1261 ttttcccaat tacacagagg cacacaagat tggtcaggtt attgcggcgg aagacgtttt
     1321 ttccgacccg cgcacctcga gcattcccag ggcgtacttt gaggacaatc ggatcaagtc
     1381 ccttcttgat gcaccggtct gggttcaggg caaactggct ggtctgctga gctttgagca
     1441 caccgagaca gtacgaacct ggcttcccga ggaagagcaa ctggctctta cccttgcgtc
     1501 gtttgtgtcg ctgtgtctgg agactcagga gcgtaagaag gcagagcagg cacttcggat
     1561 cagtgagaag aacctttcct ccatctttaa cgtgatcaag gagtctgttt ttctactgga
     1621 ctgcgaaggc atagtccttg ctgccaatca gacctttgcc gaaaggctgg cagtgacggt
     1681 tgccgactgc gttgggagac ttgtctactc tcttatacct tccgacatag cagagaagcg
     1741 tcgagccgtt gtccaacggg ttctccggac caaagagccg atcatctttg aggatgaacg
     1801 aagaggtctg t
//
LOCUS       DAOQUC010000918         1808 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_85309_length_1808_cov_2.849971, whole genome shotgun sequence.
ACCESSION   DAOQUC010000918 DAOQUC010000000
VERSION     DAOQUC010000918.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1808
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85309_length_1808_cov_2.849971"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(270..779)
                     /locus_tag="PKH07_14020"
     CDS             complement(270..779)
                     /locus_tag="PKH07_14020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36099.1"
                     /translation="MKCTIYRSLYAEAELSTEHPASSYGVPVLVILGQAYGADDILPS
                     GLTAAALVQAYLDRTESGCGEWCELSEESERSARRFLNLPEKGASEPLGLIEVTREGI
                     AEWFGGENVNRYQYLVGWYYSDQRSLAALSEVFRDRPEGDTWTRDGVTGVFASAEEAL
                     QDFRNTLTN"
     gene            complement(988..1064)
                     /locus_tag="PKH07_14025"
     tRNA            complement(988..1064)
                     /locus_tag="PKH07_14025"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1028..1030),aa:Val,seq:cac)
     gene            1399..>1808
                     /locus_tag="PKH07_14030"
     CDS             1399..>1808
                     /locus_tag="PKH07_14030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36100.1"
                     /translation="MDTNTDDKLVFSAERSFDIWDWTSPCQDLNLFENWVNDLASLGF
                     TRIEISVPWRRVEPSPGVYDFSFVSDRLAICERHKIGMRLRVNSYYGSATPDWYNGDF
                     WKTADGAYAPIRIPSINDPRFWGYYGAMCTALSR"
BASE COUNT          399 a          472 c          450 g          487 t
ORIGIN      
        1 cggcaatggt tgcatctttg gttttatatt tttgttcaaa cagtgttttg tcaacccgtg
       61 acaagacatt aggtggtgga acactctgtc ccactaccgg ctcaaacagt gttttatcaa
      121 cccgtgataa gacattaggt gcgtgtacac tctgttcgcg cacctggttt ctctgcaaag
      181 cctgtctcag tccgaggctg ttctttagcc gcttcgcttc gcttccggtt cgcctcatcc
      241 tgaatgccgg acttagttcg ttagttcgtt taattcgtta gagtattgcg aaagtcttga
      301 agcgcctctt cagccgatgc aaagactccg gtcacgccgt cgcgtgtcca ggtatcacct
      361 tctggacgat ctcggaacac ttcagacagt gcagccaatg aacgttgatc ggagtaatac
      421 cagccgacca ggtactgata ccggttgacg ttttcaccgc caaaccattc tgcaatgcct
      481 tcgcgcgtga cctcgatcaa gccaagtggc tccgatgcac ctttttctgg caggttcaga
      541 aaccgccgtg cgcttctttc gctttcttcg cttaattcgc accactctcc acagccggac
      601 tcagtcctgt ccaggtatgc ctgaaccagc gccgccgctg tcagtccaga cggtagaatg
      661 tcatcggcac cgtacgcttg tccgagaatg accaagaccg gtacgccgta ggatgatgct
      721 ggatgttcgg tagagagttc agcctctgca tacagtgaac ggtagatcgt gcatttcatg
      781 gttcgtattc ctttccatgc cggagaaaag ttctcacttt ctccgtcgct tttcactcac
      841 acgagtccga taaatttcca tgccaaaaaa cccgtgtgcc taaaacgtgc cttagatgtg
      901 cccaaaatag gcaaaaacag gcaatttcag ttgacctgac cttgaaccga tgcttgaagt
      961 aagtgttgaa ggatgtgttg tttaggctgg tgggcgatac tggacttgaa ccagtggcct
     1021 ccagtttgtg atactggcgc tctaaccagc tgagctaatc gcccgagcca ctgcactata
     1081 cttggctcac aacgtaatcc attgtgaacg aattatttgt cccacaacgg cctacgagtg
     1141 tcaaggacaa aaacttacct tcaaaccgca gattcacggt cgtggtcatt gcgcagactg
     1201 cttgtcggtg gtatcgttct gacggtttct tcgtccaaat caacgaatac tggactacac
     1261 gcaatgctca atggaatgtg ggatggttcc aggctatcac agacaggaag cagaacgcga
     1321 tgcgcggctt gcttcctgtt ggcacttctg ttcgtggtct ttgcactgct cgggagactc
     1381 gtggaggctc aaccgccgat ggatacgaat actgatgaca agttggtttt ctctgcggaa
     1441 cgttcgtttg acatctggga ttggacaagc ccatgtcagg acttgaacct ttttgagaac
     1501 tgggtcaatg accttgcctc tttgggattc acacgtatcg agatttccgt tccctggaga
     1561 agggttgaac cgtccccggg cgtctacgat ttctcgtttg tatcagaccg tctagcgatc
     1621 tgtgaaaggc acaagatcgg tatgcgtctc agggtgaaca gctattacgg cagcgctaca
     1681 cctgactggt acaatggcga tttctggaag acggcagacg gtgcctatgc tccgattcgg
     1741 attccttcga tcaacgatcc gcgtttttgg gggtactatg gggcaatgtg taccgccctc
     1801 agccgtca
//
LOCUS       DAOQUC010000919         1808 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_85313_length_1808_cov_2.792926, whole genome shotgun sequence.
ACCESSION   DAOQUC010000919 DAOQUC010000000
VERSION     DAOQUC010000919.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1808
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85313_length_1808_cov_2.792926"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            213..>1808
                     /locus_tag="PKH07_14035"
     CDS             213..>1808
                     /locus_tag="PKH07_14035"
                     /inference="COORDINATES: protein motif:HMM:NF022100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NEW3 domain-containing protein"
                     /protein_id="HNT36101.1"
                     /translation="MKSAWTTEFGWHHDFDQKKRADWTVQHLLMGVAGGYQKHGLFSW
                     AGHFGIFGDEGPKLPALSTHTLMRMLEGHRFAGVLEQDDEKWVVVWERLGKPLFMAWS
                     PKGSFKLKVPVGEEKVKLYDCFGNPLATSVSEGWLWMNLGTGIQYLVGVPGEMVNFAW
                     ESQIRSIHNRFTNHLKTTTLAEDPLLRNIADQPGPKPLEIWQALEHVSDATVEISKTQ
                     KTALQANMLRLLTQQARMGLARSFVESVTCEIGDFAPWARALLEQAHSEDVDVPALRW
                     LLNEFEDLDAERRFAESENESDFASRLRTAQVMLAAFGKKYLSDASAYELVSVWSYLL
                     AESEGDTLQERLRFTLGGTTPVQMRINSYAQRDYTASVAIQPPEGWRVSPSSFDVPIQ
                     AGADALVTFQVSCPEQVVGHHPVFHAILRIQDKPEVSVKFDDVEVVPPVRILQVPLEG
                     LLPQTPLVLGVYNEDVKSHTGQLALSLKEADQILAVTEFENLAPQSQANLVLSLTDTV
                     QPLPFQDWPLVANLVLADGKSIRQ"
BASE COUNT          390 a          453 c          525 g          440 t
ORIGIN      
        1 gtatccgaag tcagaatccg aacgctttga tcgccaacag tggaacggcc tttgtggatt
       61 tggtctgact tcgtcttcaa gccgatcggg atttgttccg ttggctggac gccgtttgcg
      121 ttcatccgta tacctgctca tcaagtccgc atgaatggaa agtatttgat gaagccaaac
      181 aggttcaggc cttcattgat gaagtgggtg gcatgaaatc agcctggact acggagtttg
      241 gatggcacca cgacttcgat cagaagaaac gcgctgactg gacagtccag catttgctga
      301 tgggagttgc cggcggatat cagaaacacg ggcttttctc ctgggcggga cattttggca
      361 tctttgggga cgaggggccg aaactgccag cgctttccac ccacacgctc atgagaatgt
      421 tggaaggaca tcggttcgcc ggagttcttg agcaggatga tgaaaagtgg gtggttgttt
      481 gggagcgtct tgggaagcca ttgttcatgg cgtggtcgcc gaaaggatcc ttcaaactca
      541 aagttcctgt cggagaggag aaagtcaaac tctacgactg ctttgggaac cctcttgcca
      601 caagcgtttc agaaggatgg ctttggatga atctgggcac aggcatccag tatctggttg
      661 gtgttcctgg ggagatggtc aactttgcct gggagagtca gattcgctcc attcacaatc
      721 gctttacaaa ccatcttaag actaccactc tggcagagga tcccttgctg cgtaacattg
      781 ccgaccagcc gggtcccaag cccttggaaa tctggcaggc gctggaacat gtttccgacg
      841 ccactgtgga aatttccaag acgcagaaga cagcccttca agcaaatatg ctgcggttgc
      901 tgactcagca agcgaggatg gggcttgcca ggagttttgt tgagagtgtg acctgtgaga
      961 ttggcgactt tgcgccctgg gcacgggcac ttttggaaca agctcatagc gaggatgtgg
     1021 acgttccggc gctgaggtgg ttgctcaacg aatttgagga cttggacgcg gagcggcgat
     1081 ttgctgaatc ggagaacgaa agcgactttg catcgcggtt gcgcaccgcg caggtcatgc
     1141 ttgctgcgtt cggcaagaag tatctgtctg atgcttcggc gtacgaactg gtttccgtct
     1201 ggtcatatct tctcgccgag agcgagggcg atacactgca ggagcgtttg cgattcaccc
     1261 tcggtggaac aacgcccgtt cagatgagaa tcaacagtta tgcccaacgt gactacacgg
     1321 cgtcggtcgc catccaaccc cctgagggtt ggcgagtgtc accttcatcg ttcgatgtgc
     1381 cgattcaggc tggtgcggac gctcttgtga catttcaggt atcctgtccg gagcaggttg
     1441 ttgggcatca tcccgtcttt catgcgatct tgcgcatcca ggacaaaccc gaagtttcgg
     1501 tgaagttcga tgatgttgaa gtcgttccgc ctgtgcgtat tctacaagtg ccgctggaag
     1561 gccttctccc ccagacgcct ctcgttctcg gcgtatacaa tgaggacgtc aagagccata
     1621 cgggacagct ggcgctttca ttgaaagagg ccgatcagat tcttgctgtt acagagttcg
     1681 aaaatcttgc accgcagtcg caagcgaatc tggtgctcag cctgacggac accgtccagc
     1741 ccctcccgtt tcaggactgg cctctcgtag ccaacctggt cttggccgat gggaagtcaa
     1801 ttcgacag
//
LOCUS       DAOQUC010000920         1807 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_85367_length_1807_cov_3.426370, whole genome shotgun sequence.
ACCESSION   DAOQUC010000920 DAOQUC010000000
VERSION     DAOQUC010000920.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1807
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85367_length_1807_cov_3.426370"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..548)
                     /locus_tag="PKH07_14040"
     CDS             complement(<1..548)
                     /locus_tag="PKH07_14040"
                     /inference="COORDINATES: protein motif:HMM:NF013067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HNT36102.1"
                     /translation="MKVLVLNCGSSSVKFQLIETDPELMEKGEEKVLAKGLVERIGEP
                     MGNLKYSATCREPLSIQAPVPRHQEAIEKILSLLTDAEHGVVKSVDEIEVVGHRMVHG
                     GEEFKASVIIDEEVLAQIEKCSRLAPLHNPHNIRGYLASRETISAVPHVAVFDTAFHQ
                     SMPKYAYLYALPYEMYTEHGIRR"
     gene            complement(561..1601)
                     /locus_tag="PKH07_14045"
     CDS             complement(561..1601)
                     /locus_tag="PKH07_14045"
                     /inference="COORDINATES: protein motif:HMM:NF014764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HNT36103.1"
                     /translation="MSQLRKDPVTGRWVAFHPERVRKAVEIVTLRSPRQRGHSCYYCQ
                     GREAETPPEIFALRQEGTAPNTPGWSTRVVPHPVPSLRVERQLVREGLGMFDRMSGVG
                     AHEVIVESPNHEADLSGLENRALEKVFLTYRDRVADLKRDQRLRYISVFKEHGIGRRF
                     MTGHSISEILGLPVTPLGIKTELNQCRQYFGYKQRCIFCDIIRDEQRMNERVVFENEG
                     FIVFEPFAARYPFETWVLPKQHQSDIVLLDSGEGVQQLAEAMRTALLLIREALLDPPY
                     GFVLHTMPNTVPRPDYWESIDQDYHWHFEIVPRVRESSGLDCASGSFVNPVLPEEAAD
                     FLRETRQRSATC"
BASE COUNT          414 a          527 c          469 g          397 t
ORIGIN      
        1 cggcgaatgc catgttccgt gtacatctca tagggcaggg cgtagaggta ggcgtatttc
       61 ggcatggatt gatgaaaagc cgtgtcgaac actgccacgt gaggaaccgc ggagatcgtc
      121 tcgcggcttg ccagataccc gcggatattg tgcggattgt gcaggggtgc cagtcgggaa
      181 cacttttcga tttgggcaag cacctcttcg tcaatgatga cggatgcctt gaactcttcg
      241 ccgccatgca ccattcgatg accgacgact tcgatctcat ccacggactt gaccacaccg
      301 tgttccgcat ccgtcagcag actgaggatt ttctcaatcg cttcctggtg cctgggaacc
      361 ggtgcctgga tggacagtgg ctcccggcac gtggcgctgt acttgaggtt gcccatcggt
      421 tccccgatgc gttccacaag acccttggca agaactttct cttcgccttt ttccatcagt
      481 tccgggtcgg tctcaatcag ttgaaacttc accgaggaac tgccacagtt gagtacaaga
      541 accttcatat cgctttcctt ttagcaagtg gcgcttcgtt gacgtgtttc ccgcaggaaa
      601 tccgccgcct cttcaggcag caccgggttc acaaacgaac ccgaggcgca gtccaacccg
      661 gacgactccc ggacgcgagg aacaatctca aagtgccagt gataatcctg atctattgat
      721 tcccagtaat caggtctcgg aactgtgttt ggcatcgtgt gcagaacgaa accatagggg
      781 ggatccaaca acgcctcacg gatgagcaac aaagccgttc tcatcgcctc tgccaactgt
      841 tgaacaccct ctccagaatc cagaaggacg atatcagact ggtgctgctt gggcaagacc
      901 caagtttcga acggatagcg cgctgcaaac ggctcaaaaa cgatgaatcc ctcgttttca
      961 aagaccacgc gctcgttcat gcgctgctcg tcccgaataa tgtcacaaaa aatgcatcgt
     1021 tgtttatagc cgaaatactg ccggcattgg ttgagctcgg tctttatccc aagtggtgtg
     1081 actggcagac ccaggatctc cgaaatggag tggcctgtca taaaccgtct tccgatgccg
     1141 tgctccttaa aaacggagat gtatcgaaga cgttgatctc tcttgagatc ggcaacacga
     1201 tcccggtagg tcaaaaagac cttctccagt gccctgttct ccagaccgct cagatccgcc
     1261 tcatggttgg gtgactccac gattacctca tgggcaccca caccactcat gcggtcgaac
     1321 atccccagtc cctcgcgcac cagctgccgc tcaaccctga gcgatggaac aggatgtgga
     1381 accacacggg tagaccatcc cggtgtgttc ggggcggttc cctcttgacg aagggcgaat
     1441 atctcgggcg gggtctcggc ttctcttccc tggcaatagt agcaggaatg cccacgctgc
     1501 ctcggcgaac gcagggtaac aatctccacg gccttgcgca ccctttccgg atggaaggca
     1561 acccagcgac ccgtgacagg atcttttctc aactgagaca tcacaacatc tgccttggac
     1621 gaccgctcaa aaaaacaccc ggaaacactc caggcgatct cgtcggcagt tccatggcgc
     1681 tttccgcaaa cgtggtacaa gggggagtgt acccaaccct gattcgagag tcaagtggga
     1741 gacacatcag ggcgtactgg ctcagttaca caagggcgga gactgtcatt ccgaacgact
     1801 gaggctt
//
LOCUS       DAOQUC010000921         1807 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_85375_length_1807_cov_3.166667, whole genome shotgun sequence.
ACCESSION   DAOQUC010000921 DAOQUC010000000
VERSION     DAOQUC010000921.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1807
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85375_length_1807_cov_3.166667"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1807
                     /locus_tag="PKH07_14050"
     CDS             <1..>1807
                     /locus_tag="PKH07_14050"
                     /inference="COORDINATES: protein motif:HMM:NF013662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HNT36104.1"
                     /translation="VAQLGAERHNVIALLQAIQNQYGYLPESVLERLCEITEITPSQI
                     VGVATFYSQFRFTPLGKHRIRVCHGTACHVKGSARIHDALHRALEIPVNQDTDAERLF
                     TVEKVACLGCCTLAPVVQIDDIVYGHLTPEKIPRMLREFLEWSDKRVAARDLSGIQGG
                     GVRPAESSLGEIRVGLGSCCVAKKSDRLRQALEDECVRLGTSVPIKRVGCVGMCHQTP
                     LVEVVAPSGRSTLYAQVSPEDARAIARKHFTPKGIVKKIRYALSDLFERVVRDDAWES
                     VERYSLDVRDAPVVSFLGRQVHIATEHFGYLNPFDIEEYERFQGFEALEKCLLRMKPD
                     GVIEEIHRSRLRGRGGAGFVSGLKWKRVRESVGEPKYVICNGDEGDPGAFMDRMLLES
                     FPFRIIEGMVIAGYAVGASEGIFYIRAEYPLALRAIREAIAVCESRGLLGGHLFDTDF
                     SFSLRVAEGAGAFVSGEETALLASLEGRRSMPRLRPPYPAEEGLWKKPTLINNVETYS
                     LVPWIISHGAESFSKLGTRSSSGTKVFALAGKVERGGLIEVPMGVSIRDIVEQIGGGV
                     AGGKRLKAVQVGGPSGGCVPAALSDTVIDFEALDSV"
BASE COUNT          382 a          417 c          565 g          443 t
ORIGIN      
        1 ggttgctcag ctgggtgcgg agcgacacaa cgtgattgcg ctcctgcaag cgatccagaa
       61 tcagtatggc tatcttccgg aatcggtgct tgagcgtctc tgtgagatca ctgagatcac
      121 accttcgcag atcgttggtg ttgctacgtt ctattcacag tttcgattca ctcctctggg
      181 aaaacaccga attcgtgttt gtcatggaac cgcttgtcat gtgaagggat cggcgagaat
      241 ccacgatgcg cttcaccgcg cactggaaat accagtaaac caggatacag acgcagagcg
      301 gcttttcaca gtagagaagg ttgcatgttt gggttgttgt accttggcac cagttgtcca
      361 gattgacgac atcgtttacg ggcacctcac accagagaag attcctcgaa tgctcagaga
      421 attccttgag tggagcgaca agcgagtcgc cgcacgcgac ctttcgggga ttcagggcgg
      481 cggtgtacgg cctgcagagt caagccttgg agaaatccga gtcggtttgg gttcgtgttg
      541 tgttgccaag aagagtgata ggcttcgcca ggctttggaa gacgaatgtg tgcgactggg
      601 aacatcggtg cctatcaagc gggttggttg cgtgggaatg tgccaccaga caccgttggt
      661 tgaggttgtc gctccaagcg gacgcagcac actttacgct caagtctctc cagaggatgc
      721 gagagccatt gcgagaaagc acttcacccc caagggaatt gtcaagaaga ttcgttacgc
      781 gctttcggat ctttttgagc gggtcgtgag agatgatgcg tgggagtctg tggaaaggta
      841 ttccctggat gttcgagacg ctcctgttgt gtcgtttctt ggaagacaag ttcacattgc
      901 gacggagcat ttcggatatc tcaacccttt tgacatcgag gagtatgagc gttttcaagg
      961 tttcgaggcc ttggagaagt gccttcttcg gatgaagccg gatggggtca tcgaggagat
     1021 ccaccgcagt cgcctgcgtg gtcgaggggg cgcgggcttt gtctctggtc tcaagtggaa
     1081 gagagttcgt gaatctgtag gtgaacccaa gtacgttatc tgcaacgggg acgagggcga
     1141 ccctggagca ttcatggaca gaatgctgct cgaatctttt ccattccgaa tcattgaagg
     1201 tatggtaatc gctggctatg cggtcggtgc cagcgaaggc atattctata ttcgggcgga
     1261 gtatccgctt gcgcttcgag ccatccgcga ggccattgcg gtgtgtgaga gcagagggct
     1321 tctgggaggc cacctctttg acacagattt ctccttcagt cttcgcgttg cagaaggggc
     1381 tggcgccttt gtttcgggtg aggaaactgc gctcctggcg tctcttgaag gcagacggag
     1441 tatgcctcga ctgcgaccgc cctatccagc tgaagaaggt ctatggaaga aaccaacgct
     1501 catcaacaac gtcgaaacct attctctggt gccttggatc atcagtcatg gggctgagtc
     1561 gttctccaaa ctgggaactc ggtcgagcag tggaaccaag gtattcgcac ttgcagggaa
     1621 ggtggaacga ggtggtctga tcgaagttcc gatgggagtc tcgatacgtg atatcgtcga
     1681 acagattggg gggggcgtgg ccggtggaaa gaggttgaag gcggttcagg ttggaggccc
     1741 ctccggtgga tgcgttccgg cggcgttgtc cgatacggtg attgacttcg aggctctgga
     1801 ttctgtg
//
LOCUS       DAOQUC010000922         1807 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_85391_length_1807_cov_2.762557, whole genome shotgun sequence.
ACCESSION   DAOQUC010000922 DAOQUC010000000
VERSION     DAOQUC010000922.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1807
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85391_length_1807_cov_2.762557"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /locus_tag="PKH07_14055"
     CDS             complement(<1..226)
                     /locus_tag="PKH07_14055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36105.1"
                     /translation="MIDINDPRITAYALGEMDEAEKGEFEKQLKDSPQARTLIAEIQS
                     LGSVLRDEFAREKTAGLTTTQREAITAKASE"
     gene            complement(241..777)
                     /locus_tag="PKH07_14060"
     CDS             complement(241..777)
                     /locus_tag="PKH07_14060"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNT36106.1"
                     /translation="MRLFTKRIEAESEHAEWVRGLLDKCEGRLIRYALRFTGNLEAAR
                     DVVQETFLRLCSTERQKIEGREVAWLFAVCRSRAVDLLRKENRMSPLDEIQLASCEST
                     EPTASESLIERERTAVVLKALAALPPREQELVRLKFQEGLSYKEISAVTQLSVSNVGL
                     LIHQGVRSLREQLKAYVQ"
     gene            complement(1179..>1807)
                     /locus_tag="PKH07_14065"
     CDS             complement(1179..>1807)
                     /locus_tag="PKH07_14065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HNT36107.1"
                     /translation="GKKRWDFKIITCHLGNGCSMAAISNGKSVDTSMGLTPLEGLVMG
                     TRCGDIDPAVVLYMIDLEGYNPEKVGGLMNKKSGLLGVSGVSNDMRELLQACEQGNER
                     AQTAVDIFCYRIKKYIGAYAAAMGGVDLVIFTGGIGENAPIVRKKCCEDLAFMNIEID
                     DQANNNVKGRDAIISNADRHVKVFVVHTNEEVVIARDSVQCVLQGAPE"
BASE COUNT          379 a          513 c          468 g          447 t
ORIGIN      
        1 tctctgaagc tttcgcggta atcgcttcgc gttgcgttgt cgtgagtcca gcagtcttct
       61 cccgggcaaa ctcatcgcgc agcaccgatc cgagagactg aatctcggcg atcaaagtcc
      121 gcgcctgcgg ggagtccttc aactgcttct cgaattcacc cttttcggct tcatccattt
      181 cgccgagggc gtaggcggtg attctgggat catttatgtc aatcatggtt catttctcct
      241 ttactgaacg taagccttca gctgctcccg aagggatctc actccctggt ggatcagaag
      301 tccgacattg ctgacggaaa gctgggtcac ggcgctgatc tccttgtaac tcagaccttc
      361 ctgaaacttc agtctgacca actcctgctc tctgggggga agagcggcaa gcgccttgag
      421 cacgacggca gtgcgctctc tttcgataag agattccgat gccgtcggct cggtactctc
      481 acaagacgcc aactgtattt catcaagagg actcatccga ttctcctttc gcaatagatc
      541 cacggcgcgg cttcggcaca cggcaaagag ccaggcgacc tctcggcctt caatcttctg
      601 gcgctctgtg gaacacagtc tcaggaaggt ctcctgcacg acgtctcgtg ccgcttccag
      661 gttaccggta aaccggagag cgtaacggat caaccgtccc tcacacttgt ccagtagtcc
      721 tcgcacccat tcggcgtgct cagactccgc ttcgatgcgc tttgtgaaga gtctcattgc
      781 cattcctctg atgaaacgat cccaacagag ttttcttagg agaaatccac tgtgatgaca
      841 tgttatcaga atttggggat cggtgggata gaggaggatt ggcggatgcg agtctgtgtt
      901 ggtttggaac aagtttcggt aggatggatc accaatcccg cccataaagg agaaaagcgg
      961 tagcaagcta ccgcactccg ggaaacggat tttggagtgc gcaagcttgc ttgcgcgggg
     1021 cgtatcgtcc tgggaacgcc gggcgcctgc tcggcagtcc ggtagttccg gtaggatggc
     1081 tcgtgatcca tcctaccgga ctcccgatga acgacaaacc ctggaaatct ccaaggattt
     1141 ccagggtttc tgttgttacc cgcgattggt ctgtggcgct attcgggtgc gccttgaagg
     1201 acacactgaa ccgaatcccg cgcgatgacg acctcttcat tggtgtgaac gacaaagact
     1261 ttgacatgtc tgtcggcgtt ggagatgatc gcatcacgtc ccttgacgtt gttgttggcc
     1321 tggtcgtcta tttcgatgtt cataaacgcc aaatcctcac agcacttttt ccggacaatc
     1381 ggagcgtttt cgcctatgcc ccctgtgaag atgacaagat ccaccccacc catcgccgcg
     1441 gcataggctc cgatgtattt cttgattcgg tagcagaaaa tgtccacggc agtctgagca
     1501 cgttcgttgc cctgctcgca tgcctgaagc agttcgcgca tgtcattgct gacacccgag
     1561 acacccaaaa gaccgctctt cttgttcatg aggccgccaa ccttttcggg gttatagcct
     1621 tcaaggtcaa tcatgtagag cacgacggca ggatcaatgt ccccgcaacg tgttcccatc
     1681 accaagcctt ccaagggggt cagtcccatt gaggtgtcaa cggacttgcc gttgctgatg
     1741 gctgccatac tgcatccatt acccagatga caggtgatga ttttgaaatc ccatcgcttc
     1801 ttgccca
//
LOCUS       DAOQUC010000923         1805 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_85550_length_1805_cov_3.186286, whole genome shotgun sequence.
ACCESSION   DAOQUC010000923 DAOQUC010000000
VERSION     DAOQUC010000923.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1805)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1805)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1805
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85550_length_1805_cov_3.186286"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..434)
                     /locus_tag="PKH07_14070"
     CDS             complement(<1..434)
                     /locus_tag="PKH07_14070"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNT36108.1"
                     /translation="MTSVLIVDDSSLFRTLIRRALEDIPGVQVVATAPSGRLALKRIE
                     ELSPHLLTLDLEMPDLDGIGVLKALKERSLDVGVIILSSHTRKAGELTMKALELGAFD
                     FIAKPEALSADEAQAALRESLRPMIAAYARKIEVRSILQNRPL"
     gene            complement(581..1048)
                     /locus_tag="PKH07_14075"
     CDS             complement(581..1048)
                     /locus_tag="PKH07_14075"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HNT36109.1"
                     /translation="MATNVTDQTAQEAQSSLILTCYVGEILLGINALKVQEVIKTPEH
                     TPVHHAPDYVIGVMNLRGRIVTLIDLGRRMDANHLEDSSESRIVIVDFMNETVGLLVD
                     KVSDVITPEESALAEAPSNIRGAKEKFIQGICRCPKGLVAVLDTDRILSEREA"
     gene            complement(1091..>1805)
                     /locus_tag="PKH07_14080"
     CDS             complement(1091..>1805)
                     /locus_tag="PKH07_14080"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNT36110.1"
                     /translation="MEQTSVSLTTVATATEEMTATIGEIARNSENGRAISVEAASQAD
                     RISSMMQGLSQAAEEIGKVTETITSISSQTNLLALNATIEAARAGSAGKGFAVVANEI
                     KELAQQTSSATEDIKSRIGGIQSSTRATISDIERIVQVIRQITDIVSSIAAAIEEQSV
                     VTKDIAGNIAQAAQGVNDVNDRVAQSSAVSQGIAKDIAAVNQSASEISGGSSIVHISA
                     EEFARMAKELSEIVGRFRV"
BASE COUNT          382 a          504 c          473 g          446 t
ORIGIN      
        1 agaggtctgt tctgaaggat tgagcggact tcaatcttcc tggcataggc ggcgatcata
       61 ggtcgtagag actcacgaag ggctgcctgc gcttcgtccg ccgacagtgc ttcgggtttt
      121 gcaataaaat cgaaggcacc caactcgaga gccttcatgg taagttctcc agccttgcgg
      181 gtgtggctgc tgagaatgat gacgcccacg tccaaggagc gttccttgag tgctttcagg
      241 actccgatac cgtccagatc aggcatctcg agatcgagag tgagcagatg ggggcttagt
      301 tcttcaattc gtttcagagc caggcgtccg ctgggtgcag ttgcgacgac ttgaacgccg
      361 ggaatgtctt ccagcgccct gcggataagc gttcgaaaca gggaactgtc atccacgata
      421 agcacggaag tcatcttgca tttctcccta tgacactcag tccagagcga ggatgtcctt
      481 tcccgatcaa gcgtccaagg gtttgggcat agcccacgcg ccccgcaagt gccaacctga
      541 tcgacatggc gaaggtgcca tggatcacat cagttcaaag tcaggcttcg cgctcagaca
      601 ggatacgatc cgtatccagt acggcaacca gccccttggg gcaacggcaa attccctgaa
      661 tgaacttctc tttggctcca cgtatgttgg acggcgcctc tgccaacgca gactcttcgg
      721 gtgtaatcac atccgagact ttgtcaacaa gcaatccaac tgtttcgttc atgaagtcaa
      781 cgatgacgat ccggctctct gaagagtcct ccagatggtt cgcgtccatt cgtctgccga
      841 gatcgatcaa cgtcacgatt cgacctcgca gattcatgac tccgatcacg taatcaggag
      901 cgtggtgtac cggtgtatgt tcaggtgtct tgataacttc ctgaaccttc agtgcattga
      961 ttcccagaag aatctcgcct acatagcagg tcaggatgag agatgactgg gcttcttggg
     1021 cagtctgatc ggttacattc gttgccattg tcctgcgctc ctgtctcgcc agaagtggtt
     1081 ctgcacgagt ctacaccctg aacctgccta cgatttcact cagttccttt gccatgcggg
     1141 caaactcctc agcagagata tgcacaatgg aactcccacc ggagatctcg ctggcagact
     1201 gattgaccgc agcgatgtcc ttggcaattc cttgcgaaac ggcggaactc tgagcaaccc
     1261 gatcgttgac gtcgttgacg ccctgagctg cctgggcaat gtttccggca atgtctttgg
     1321 ttacaacgga ctgctcttcg atcgccgcgg cgattgatga cacgatgtca gtaatctgac
     1381 ggatgacctg aacgatcctc tcgatatccg aaatcgtggc acgtgtagat gactgaatgc
     1441 ctccgatgcg actcttgatg tcttctgtcg ccgaggaagt ctgttgcgcc agttccttaa
     1501 tctcgttggc gacaacggca aaccccttcc cggcgcttcc cgcccgcgcc gcttctatgg
     1561 tggcgttgag tgcgagcaag tttgtctgtg aagagatgct cgtaatcgtt tcggtcacct
     1621 tgccaatctc ttctgccgcc tggctcaagc cctgcatcat cgaagatatt ctatccgctt
     1681 gcgaagcagc ttcgacgctg atggctcgac cattctcgga gttgcgtgca atttcaccaa
     1741 ttgttgcagt catctcctca gtcgcggtgg caactgtggt caggctgaca gaagtctgct
     1801 ccatg
//
LOCUS       DAOQUC010000924         1804 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_85608_length_1804_cov_4.050886, whole genome shotgun sequence.
ACCESSION   DAOQUC010000924 DAOQUC010000000
VERSION     DAOQUC010000924.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1804)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1804)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1804
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85608_length_1804_cov_4.050886"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(169..>1804)
                     /locus_tag="PKH07_14085"
     CDS             complement(169..>1804)
                     /locus_tag="PKH07_14085"
                     /inference="COORDINATES: protein motif:HMM:NF021570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2298 domain-containing protein"
                     /protein_id="HNT36111.1"
                     /translation="SVCIIIGCEFVAMKDFYGDASARMNTVFKFHFQAWLFLAIAGAM
                     GLGEIVERLKGNWGWKATGAVWLVALVLLLQACSVFTWRATMAKTNNWKAKPTLDGLR
                     YLKETSSGDYAAIMWLREQTKDLRGSDAPLVLEATKDPYSDFSRFSTFTGLPSLVGWA
                     NHEGIWHRNNTGKNPHSRPAIVKDIYSNVNFGAARKVLEQYNVKYVIVGSLEQQEFDS
                     TELGKFPKYMHTVFEQGTTAIYSGYLEPERSDYPDEEQPIDKRKAAKRLDLVKIVGSK
                     GGDPGQFQEVRGIAAAPDGSLYTVDKALHRVQKFDANGNYVKEWGTQGGDPSQFNAPH
                     GIAVDADGNVYVADTWNHRIQKFDAEGHFLVSWSGEFWGPRDLAIAQDGTVFVADTGN
                     KLIKTFTKDGTPLKRWGGDGNDRGKMVEPVGIAVHDDKVYVCDVGNRRIQAFTFDGEF
                     VTMWPVVSGWREFFTEPYLAIDLQGHLVVSDSKYNRLHCYSLTGEFVDFYGMRGTGNS
                     EFDLPMGVAFTPDGHLAVGDVNNGRVQILALPQGSQ"
BASE COUNT          391 a          515 c          456 g          442 t
ORIGIN      
        1 cgtgcagggg cactccgcga aaagagcgga gcgggaagag atgtcctgct cagagagttg
       61 gaactaccga acgaaaagag cactgtcttg taagatgcat ctgcttcagg caagattggt
      121 gatcccgcag aacaaaaaac agggtcgcct ctgacctctg cgctgtgctc actgagaccc
      181 ctggggcaaa gcaagtattt gcacccgccc gttattcaca tcaccgactg cgagatgtcc
      241 gtcgggggtg aatgctacac ccatcggtag atcgaactct gagtttcccg ttcccctcat
      301 tccgtagaaa tcaacaaact ctccggtcag gctgtaacaa tgaagacggt tgtacttgga
      361 gtcggagacg accaagtggc cctgcaagtc aatggcgaga tagggttcgg tgaagaactc
      421 gcgccaacca ctgacgaccg gccacatagt gacaaactcc ccgtcgaagg tgaaggcctg
      481 tattcttctg ttgcctacgt cgcagacgta gactttgtca tcatgaactg cgatgccaac
      541 cggttccacc atcttgcctc tgtcattgcc atcgcccccc cagcgtttca gtggtgtccc
      601 gtctttggta aaggttttga tcaacttgtt gccggtatct gccacaaaga ctgttccatc
      661 ctgggcaatc gccagatccc gcggtcccca gaactctccc gaccatgaaa ccaggaagtg
      721 accttcggca tcgaatttct ggatgcggtg attccatgta tcggcaacat agacgttgcc
      781 atcggcatct actgcgattc catgtggagc gttgaactgg ctcgggtcgc ccccttgtgt
      841 gccccattcc ttgacgtagt tgccgttggc atcaaacttc tgaacacgat gcaatgcctt
      901 gtccaccgtg tagagacttc catctggtgc cgcggcaatc ccacgcactt cctggaattg
      961 accagggtca ccacctttgc ttccgacaat cttcaccaaa tcgagcctct tggccgcctt
     1021 tctcttgtca atgggctgtt cctcatcggg atagtccgag cgttccggct ccaggtatcc
     1081 ggagtagatt gccgttgtcc cctgttcaaa aaccgtgtgc atatactttg ggaacttgcc
     1141 gagttcggtg ctgtcgaact cctgttgctc cagtgaaccg acaatgacgt atttgacgtt
     1201 gtattgttcg aggacttttc gcgccgcacc gaaattgaca ttgctgtaga tgtccttgac
     1261 gatggcaggg cggctgtgtg ggttctttcc tgtattattg cgatgccaga taccctcatg
     1321 gtttgcccaa ccgacgagac ttggaagtcc ggtgaacgtc gagaatctgg agaagtccga
     1381 gtagggatcc ttggttgcct cgagcaccaa tggggcgtcc gaaccccgca gatcctttgt
     1441 ctgttctctg agccacatga tggcggcgta gtctccggat gatgtttcct tcaggtatcg
     1501 aagcccatcg agtgttggtt tggccttcca gttgttggtc ttggccatcg ttgctcgcca
     1561 ggtgaacacg ctgcatgcct gaagcaacag aacaagtgcg accaaccaaa cggctcctgt
     1621 tgccttccag ccccagtttc ccttcagtcg ctccacaatc tctcccagtc ccatggctcc
     1681 ggcgattgcc aggaacagcc aagcctggaa atggaacttg aacactgtgt tcatccgggc
     1741 actcgcatcg ccataaaaat ccttcatcgc cacaaattca catccgatga tgatgcaaac
     1801 tgaa
//
LOCUS       DAOQUC010000925         1803 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_85696_length_1803_cov_3.267735, whole genome shotgun sequence.
ACCESSION   DAOQUC010000925 DAOQUC010000000
VERSION     DAOQUC010000925.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1803)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1803)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1803
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85696_length_1803_cov_3.267735"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..547)
                     /locus_tag="PKH07_14090"
     CDS             complement(<1..547)
                     /locus_tag="PKH07_14090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36112.1"
                     /translation="MNHPTTPETSDSAVPEFNGAVPSDGLRISTYRPRIAFGAGYQSF
                     VDLPTGGLEWRETYCYELEQTVPPGSVGSVVTGFRKGVTSIVIQGEIQADDPESVATT
                     LSGIESAVACGAFRLFSHYSPDDGVYRYYQDCYATKFISDLRAGDPHRPGRNTRYELH
                     LIATDPSVQGTEPSEDSNLFVG"
     gene            complement(544..1365)
                     /locus_tag="PKH07_14095"
     CDS             complement(544..1365)
                     /locus_tag="PKH07_14095"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNT36113.1"
                     /translation="MRSGSVCCPIGLCLLTYNSVSLGLTRGGSELEIRVHVNERCTDE
                     YGDVPVTLYFAGATAFLHTHIAEHTVQLLNRIIPGSSLVGGTVLSIGGCVGKEIPAYP
                     LVITPVDARDPVGQVTLHRAVALPTGRIAYQPGKERVIPVVFRGLVDPFLEDGHRLVT
                     WSSSKDQTPPEIISSTPQDEETEVSTETSIEIKFSESVGPISSETFIVLDETNNQIVP
                     CCRQWELIPNFVVTLIPESALAHSAVHRVIVAGVADAAGNPMDIRILRFTTEHAE"
BASE COUNT          428 a          502 c          489 g          384 t
ORIGIN      
        1 cccccacaaa cagattcgaa tcctcggaag gctctgtacc ttgaacgctg ggatcggtcg
       61 cgatgagatg aagctcgtag cgggtattcc gacccggtct gtggggatct cctgcgcgaa
      121 ggtcactgat gaattttgtt gcgtagcagt cctgatagta tcggtacacc ccgtcgtcgg
      181 gactgtaatg agaaaagagt cggaaggctc cacacgcaac ggcggattca atgccgctca
      241 gtgtcgtcgc cacagactcg ggatcatccg cctggatctc cccttggatg acaatgcttg
      301 tcaccccttt ccgaaaacct gtgaccacac tgccgactga gcctggtggc acagtctgct
      361 ccaattcgta gcagtaggtc tctcgccatt ccaagccacc ggtgggaaga tccacaaacg
      421 actgatatcc cgccccaaaa gcgattcggg gtctgtatgt tgagatgcga agaccgtcag
      481 agggaaccgc cccattgaac tctggcacgg cggaatcact ggtctcggga gtagtcggat
      541 gattcattct gcatgctccg tggtgaatct cagaatgcga atgtccatgg gatttcctgc
      601 agcgtccgcc acacccgcaa cgataactcg gtgtactgca gagtgagcga gcgcagactc
      661 cgggatcaga gtgacaacga aattcggtat cagctcccac tgtcggcagc agggcacaat
      721 ctgattgttg gtctcatcca agacaatgaa agtttcggac gagatcggtc ccaccgactc
      781 ggagaactta atttcgattg atgtctcagt agaaacttcc gtttcttcat cttgcggtgt
      841 actgctgatg atctcaggcg gagtctgatc tttggaactc gaccatgtca ccagtcggtg
      901 tccgtcttcg agaaacggat ccaccagacc gcgaaacaca acagggatga ctcgttcctt
      961 gccgggttga taggcaatcc gtcccgtcgg gagcgccacg gcccgatgta gcgtcacctg
     1021 tccgacagga tcccgcgcat caaccggagt gatcactaac ggatatgccg gaatctcctt
     1081 tcccacacag ccgccaattg aaagaaccgt accgcccacc agtgaggaac ccggaatgat
     1141 cctgttgagc agttgaaccg tatgctccgc aatgtgtgtg tgcagaaagg ctgtcgcacc
     1201 ggcgaagtac agggtgactg gaacatcacc gtactcatcg gtacaccgct cattgacatg
     1261 aacacggatc tcaagttctg aacccccacg cgtcaaaccg agggaaaccg aattgtaggt
     1321 gagaagacaa agaccaatgg gacaacaaac tgatccagat ctcatggtcg ccatccttgt
     1381 ttgcggtgcc cgtccgctgg ggagccttgc atgttcttga ctgtctccat tcgacccaac
     1441 gagtcttcac tctgagtttg ttgaggatcg tcaccaaagg gcaagggaag acaagcgcta
     1501 caaagcgaca ccaagcgaca gatgaagaca tacccttacg cgtttgtaga ttcggagatg
     1561 ccgcttgacg acaaccgccg gtcaatgttg ttattgacag acttgggcga gggtcgcaca
     1621 ctctcccttc aagacggatt ctgagaaacg agcgtctcgc gggtagatgg gctgagtcca
     1681 gcgtttggga cgctcacacg aatgggagac cgctcttgtc tggagcgttg tctcctggga
     1741 ggcacagtca tgacagcgga gctttcaaca cgctacgatc cccaggcagc acaagcccgg
     1801 tgg
//
LOCUS       DAOQUC010000926         1803 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_85713_length_1803_cov_2.664188, whole genome shotgun sequence.
ACCESSION   DAOQUC010000926 DAOQUC010000000
VERSION     DAOQUC010000926.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1803)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1803)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1803
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85713_length_1803_cov_2.664188"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..609
                     /locus_tag="PKH07_14100"
     CDS             <1..609
                     /locus_tag="PKH07_14100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015819569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HNT36114.1"
                     /translation="TPALVEEAMTVARQAGTIVSYDLNYRASLWKDIGGQEKAQEVNR
                     SLASLVDVMIGNEEDFSAALGFSVEGLDSNFSKLDPTNFQRMIQQVVKQYPNFKVVAT
                     TLRNAKTATINDWGAVLYCDGRFHEATQRLDLEIYDRVGGGDSFASGLIYAFLQGKDP
                     QQAVEYGAAHGALAMTTPGDTTMATLDEVERVMKGGAARIAR"
     gene            625..1266
                     /gene="eda"
                     /locus_tag="PKH07_14105"
     CDS             625..1266
                     /gene="eda"
                     /locus_tag="PKH07_14105"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008101467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008675 -
                     2-dehydro-3-deoxy-phosphogluconate aldolase activity
                     [Evidence IEA];
                     GO_function: GO:0008700 - 4-hydroxy-2-oxoglutarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0009084 - glutamine family amino acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HNT36115.1"
                     /translation="MITDRIEKSRIIPVIALEKAEDAIPLCKALGAGGLHVAEITFRT
                     AAARDALKIVSQEFPEFALGAGTVTTLEEVETAVNCGAQFAVAPGLNPRVVKKAQELG
                     LPFFPGVCTPTDIEMALECGCQTLKYFPAGEMGGISMLKALYGPYRHKGVRFIPTGGV
                     SVTNLADYIKEPSVVAVGGTWIASAGLIKAKNWDEISNLARSAVEIVRSAIVS"
     gene            1322..>1803
                     /locus_tag="PKH07_14110"
     CDS             1322..>1803
                     /locus_tag="PKH07_14110"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNT36116.1"
                     /translation="MEGPRPVRLDEMSSLSQLVDTVFRSGRDSTMFGEFPHYFHPDNL
                     ENLFVFVDQGRVVSHFGMTRRWASLEGSTVGVASVGAVATYEEYRGKGLATQLFEHSR
                     REMIAHGIDFMLISGGRGLYRRAGAADFGTNYTGVLDPGSISLPAERGITISEFEHAD
                     "
BASE COUNT          404 a          407 c          545 g          447 t
ORIGIN      
        1 actcccgcat tggtcgaaga ggcgatgacc gtcgctagac aggctggcac catcgtttcc
       61 tacgacctaa attatcgtgc atccttgtgg aaggatattg gtggacagga aaaggcgcag
      121 gaagtcaacc gcagtcttgc ctcgcttgtg gatgttatga tcggcaatga ggaagacttc
      181 agcgcagcct tgggattcag cgttgagggg cttgactcaa atttctccaa actggatccc
      241 acgaattttc agcgaatgat tcagcaggtt gtgaagcaat atccgaattt caaagtggtt
      301 gccacaaccc tacggaatgc caagacagcg accatcaacg actggggcgc ggtgctctat
      361 tgtgacggac gattccatga ggcaacgcag cgtctcgatc tggagatcta tgatcgtgta
      421 ggtgggggcg acagtttcgc gtcgggtctg atctatgcct ttctgcaggg caaagatcct
      481 cagcaggcgg tggagtacgg tgctgctcac ggtgcactgg ccatgaccac acctggggac
      541 accacaatgg cgacgcttga tgaggttgag cgtgtgatga agggtggcgc tgcgcgaatt
      601 gcgcgctaag aaggagcggt aagtatgatt acagatagaa ttgagaaatc acgcattatt
      661 cctgtcattg cactggagaa ggccgaggat gcgatcccgt tgtgcaaggc tctcggtgcc
      721 ggaggcttgc atgtggcaga gatcacattt cgaaccgccg ccgcacgaga cgcactgaag
      781 atagtctcgc aggaattccc tgagtttgca cttggcgcag gaaccgtgac gactttggaa
      841 gaagtggaga cagcggtcaa ttgtggcgca cagttcgcag ttgcgccagg tttgaatccg
      901 cgagtggtca agaaggcgca ggaactcgga cttccatttt tcccaggtgt ctgtactccg
      961 acagacattg agatggcttt ggaatgtggc tgtcagactt tgaagtactt tccagcgggt
     1021 gagatgggtg ggatcagtat gctcaaggcg ctctacggtc cctatcgtca taaaggagtt
     1081 cgattcattc ctacgggtgg agtgagcgtt acgaatctgg ccgattacat taaggaacct
     1141 tctgtggttg ccgtgggagg aacctggatc gcttcagccg gccttatcaa ggccaagaac
     1201 tgggacgaga tatccaatct tgctcgaagt gctgtggaga ttgttcgttc tgctattgta
     1261 tcatgagcac ttgattctcg cttgtgtcaa gtgcagagaa cctgcgtgag aggagtctat
     1321 cgtggaagga ccgagacctg tgcggttgga tgaaatgtca tcgctgagtc aattggtgga
     1381 cactgtgttt cgttctgggc gtgactcaac gatgttcggg gagtttcctc attattttca
     1441 tcccgacaat ctggagaatc ttttcgtttt tgtggatcaa ggaagagttg tcagccattt
     1501 tggcatgaca aggcgctggg cgtcgcttga gggtagcact gttggagtgg cgagtgttgg
     1561 tgccgtagcc acctatgaag agtatcgagg caagggtctg gctacgcagt tgtttgaaca
     1621 cagtcgccgt gagatgatcg ctcatggcat tgacttcatg cttatctcag ggggacgggg
     1681 tctttaccgg agagcaggtg ccgcggattt cggaaccaac tacaccggag ttcttgaccc
     1741 tggttccatt tctcttcccg cggaacgagg gatcaccata tcggagtttg aacacgctga
     1801 ctt
//
LOCUS       DAOQUC010000927         1803 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_85718_length_1803_cov_2.518307, whole genome shotgun sequence.
ACCESSION   DAOQUC010000927 DAOQUC010000000
VERSION     DAOQUC010000927.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1803)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1803)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1803
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85718_length_1803_cov_2.518307"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /locus_tag="PKH07_14115"
     CDS             complement(<1..331)
                     /locus_tag="PKH07_14115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36117.1"
                     /translation="MRPTLTRQDRFGFVHPALDAHTLGISSMAQILAECGFESVQADA
                     TVCEAVGALDNPASIPIIERWLRDDRITALGFSYRLDPEDGAAACGRLVSQMKRRRLF
                     TDQGGTVK"
     gene            complement(344..1450)
                     /locus_tag="PKH07_14120"
     CDS             complement(344..1450)
                     /locus_tag="PKH07_14120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD/NADP octopine/nopaline dehydrogenase family
                     protein"
                     /protein_id="HNT36118.1"
                     /translation="MPTFCVLGAGHGGMAMAGHLSLMGCKVHLFNRTPERLAPVLARG
                     GIEVTGQVEGFAPVHLITSDPQDAVQDADVLMVVVPATGHRSIVEAIAPHVKDGQILV
                     LNPGRTGGSLEAARILRERNPKTRVFIGEAQTLLYASRVTNPGLVHILRIKNSVPIAT
                     LPAYDVTDILPVIHKALPQFVPGDNVLKTSFDNIGAVFHPAITILNAARIEESHGDFE
                     YYTEGVSHAVASVLEAIDAERVAVAGALGIRANTAREWLYLAYDAAGRTLLDAMHANT
                     GYAGIKAPRTVQHRYITEDVPASLVPLASIGDMLNVPTPTIHAMIHIASIMHGTDYWA
                     EGRTAESLGIKGMSVKDIRLLVVGAEPATATNPV"
     gene            complement(1550..1744)
                     /locus_tag="PKH07_14125"
                     /pseudo
     CDS             complement(1550..1744)
                     /locus_tag="PKH07_14125"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
BASE COUNT          398 a          536 c          512 g          357 t
ORIGIN      
        1 ctttgaccgt tcctccctgg tcagtaaaca ggcgacgcct cttcatctgg gaaacaagac
       61 gcccacatgc cgcagcgccg tcctcgggat ccagccggta actaaatccc aaggctgtga
      121 tgcggtcgtc acgaagccat cgctcgatga tcggaatgct tgccggattg tccagtgcgc
      181 caacggcttc acatactgtg gcgtctgcct gtacggactc gaagccgcac tcggcgagaa
      241 tctgagccat tgaactgata ccgagggtat gggcatccaa ggcaggatgg acaaaaccaa
      301 aacggtcttg acgagtaagg gtcggtcgca tggcagcctc cctttacaca ggattcgtcg
      361 cagttgcggg ttcggctccg accacgagaa gccgaatgtc cttcacggac attcctttga
      421 tgccaaggct ctccgcggtt ctcccttccg cccagtagtc ggtgccatgc atgatcgaag
      481 cgatgtgaat catcgcgtga atggtcggcg ttggaacgtt cagcatatcc ccgatggacg
      541 cgagcgggac aagacttgcg ggcacgtctt ccgtgatgta gcggtgttgg actgttctgg
      601 gagccttgat gccggcatat cccgtgttgg catgcatggc atccaggagt gtcctgcccg
      661 cggcatcata ggcaagatac agccattcgc gcgccgtgtt ggcgcgaatg cccagcgcac
      721 ctgctaccgc gacgcgttcc gcatctatcg cttcaagaac cgaggcaacg gcatgggaaa
      781 ccccctctgt gtagtattca aagtcgccgt gactttcctc aattcgcgcc gcgttgagaa
      841 ttgtaattgc gggatgaaaa accgcgccga tgttgtcaaa actggtcttg agcacattgt
      901 ctcccgggac gaattgcgga agcgccttgt gtatcaccgg caggatgtcg gtcacgtcat
      961 aggcgggaag cgttgcgatg ggcaccgagt tcttgatccg aagaatatgc accaaaccgg
     1021 gattggtcac ccgagaagcg tacaacagcg tttgggcttc cccgatgaac acgcgagttt
     1081 ttggatttcg ctccctgaga atccgggctg cctccaatga gccgcccgtg cgtccaggat
     1141 tcaaaaccag gatttggcca tctttgacgt ggggagcaat cgcctcaacg atactccgat
     1201 gcccagtggc cggcacgacg accatcaaca catcggcatc ttgaacggca tcctgcggat
     1261 cggacgtgat tagatgaact ggcgcaaagc cttccacctg tccggtcacc tcgattccgc
     1321 cacgagcgag aaccggcgca agcctttcgg gagtgcggtt gaacaaatgc accttgcatc
     1381 ccatgagaga aagatgtcct gccatggcca tgccaccatg cccggcaccc aacacacaga
     1441 acgtgggcat cgcttcagca gagcgcatct cgcccaatgc ttcttccgcc tcactgaaca
     1501 gagagatcac ctcgtgagaa gtcttggcgt tcaggagcgc ctcaccgaaa tcctcagcgc
     1561 gacacagtcg tgcgaggcgt cccagacagc gcaggtagag agcatgtgcc tgcggtggcc
     1621 ccaacagaac gaagaccaga cgtgccctct cacctctgga gccccaggga ataccgtccg
     1681 gcgagacgcc aacggcgatg acgatctgct ggcaggcttt tgaagaggca tgaggcagcg
     1741 ccatcaagtg agatccgacc ggcagaagga tagaccctgc gtggtcacgc gcattcagat
     1801 ccc
//
LOCUS       DAOQUC010000928         1799 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_85966_length_1799_cov_4.635894, whole genome shotgun sequence.
ACCESSION   DAOQUC010000928 DAOQUC010000000
VERSION     DAOQUC010000928.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1799
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85966_length_1799_cov_4.635894"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..441)
                     /locus_tag="PKH07_14130"
     CDS             complement(<1..441)
                     /locus_tag="PKH07_14130"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HNT36119.1"
                     /translation="MTGEGKRPNALRIWVRAFRPFAYPASLLPILVGTFAVTPFPAVR
                     WGLLTMVLLAVFLMHSGGNLLNEYFDYKSGVDLKVHPEAGRFWGALPSGFLRPVQVLI
                     AAFVCLTLSGILGLIIVMTTGIWSLLLGIIGAVGLYAYTGPPFQL"
     gene            complement(438..>1799)
                     /locus_tag="PKH07_14135"
     CDS             complement(438..>1799)
                     /locus_tag="PKH07_14135"
                     /inference="COORDINATES: protein motif:HMM:NF036968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HNT36120.1"
                     /translation="CNLLRRLRCRDFECLIASLSLIRPGASNQNKVEHYARRHSGLDP
                     SQYIHPSVQAAMKDTYGLMVYEEHILMVAHLFAGMDLGRADLLRRALVKQSDREKAAG
                     FEVEFRELAIRKGRNQEEIDQVWKFLLDFCGYSFNKAHSSSYAVLAYQAAYLKSHFPA
                     EFMASVLSSGKGFYSRLTYVLESLHLGIAVDGPNVNSSQEDYFARNGRICVPLSQIKD
                     LTQKTIRRTLKARRPFSDLADFLLKVEPSPSELDSLIRSGACDTFGENRPALLWMART
                     SGIAGTPSLKGESADEGNLLTCIENRQYHSPERPKYSAVSCPIPDYTLAKKIRDEMDL
                     LGFPLSGHPLDAIDPSGVQSDCQTSRDLEKAKTAVSLCGIIVAERSHKTQGGSWMKFA
                     TLSDRTGMIEVVLFPEVSRKYAPLIGLGKIVLISGTIQRFEHKRVGSLIAEVVRNLGE
                     FEL"
BASE COUNT          459 a          459 c          475 g          406 t
ORIGIN      
        1 gagttgaaag ggtggtccgg tgtaggcata gagtccgaca gcaccaatga tcccgagcaa
       61 cagactccag atacccgttg tcataacgat gatgagcccg agaattcccg agagggtcaa
      121 acagacaaaa gcggcgatga gtacctgaac aggcctgagg aagccactcg gcagtgcgcc
      181 ccagaaccga cccgcctcgg gatgtacctt gagatcaaca cccgacttgt aatcgaaata
      241 ctcgttgagc agatttcccc ctgagtgcat cagaaagacc gccaggagca ccattgtcag
      301 aagtccccat cgaacagcag gaaagggggt aacagcaaat gtcccgacca ggatgggaag
      361 cagtgaagca ggatatgcga aaggtcggaa agcacggacc cagatcctca aagcgttggg
      421 gcgtttacct tcaccagtca tagttcaaac tctcccaagt tcctcaccac ttccgcgatc
      481 agtgagccaa ctcttttgtg ttcaaaccgt tgtattgtac cagaaatgag aacaatcttg
      541 ccaagtccga tcaagggagc gtacttgcga gacacttccg gaaagagcac aacttcaatc
      601 atgccggttc gatcagacag cgttgcgaac ttcatccaac tgcctccctg tgttttgtga
      661 gatcgttcgg caacaatgat gccgcagaga gaaacagctg ttttcgcttt ctccaaatcc
      721 cgtgatgttt ggcagtcgct ctgaacgcca ctcggatcta tcgcatcgag cggatgacct
      781 gaaaggggga atcccagtag atccatctca tcgcgaatct tctttgccag cgtgtagtcg
      841 ggaattggac aagaaacggc tgaatacttc ggtctctctg gagagtgata ctgacgattc
      901 tcgatgcacg tgagaagatt gccctcgtct gctgattctc ccttcaggct cggcgttcca
      961 gcgatgcctg acgtgcgagc catccagagc aatgccggac ggttctcgcc aaatgtatcg
     1021 catgcgccgc ttcggatgag actgtcaagt tccgatggac taggttcgac cttgagaaga
     1081 aagtcagcca gatcagaaaa gggtctgcgc gccttcagtg ttcgtcgaat cgtcttctgt
     1141 gtaaggtctt tgatctgcga aagaggaacg cagattctgc catttcgagc gaagtagtct
     1201 tcttgggagc tgttgacatt cgggccatca acggcaattc ccagatgcaa agactccagg
     1261 acataggtca atcgggagta gaaacctttt ccgctggaga gtaccgatgc catgaattcg
     1321 gcggggaaat ggctcttgag ataggctgcc tgataggcga gaacagcata actgctcgaa
     1381 tgtgctttgt tgaaggaata gccgcagaag tccagaagga atttccagac ttggtcaatc
     1441 tcttcctgat tgcgtccttt ccggatcgcg agttctctga attccacttc aaagccagca
     1501 gccttctctc ggtcggattg cttgaccaag gctcgccgga gcagatcggc acgtccgaga
     1561 tccatccctg caaacaggtg agcgaccatg agtatatgct cctcgtagac catcaatccg
     1621 taggtgtctt tcatggctgc ttgaaccgat ggatgaatgt actgggaagg gtctagtcca
     1681 gaatgccttc gggcatagtg ttccactttg ttttgatttg aagcaccggg ccggataagg
     1741 ctcaaagagg caatcagaca ctcaaaatct ctgcatctga gacggcgaag aaggttgca
//
LOCUS       DAOQUC010000929         1799 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_85998_length_1799_cov_2.949541, whole genome shotgun sequence.
ACCESSION   DAOQUC010000929 DAOQUC010000000
VERSION     DAOQUC010000929.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1799
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85998_length_1799_cov_2.949541"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..367)
                     /locus_tag="PKH07_14140"
     CDS             complement(<1..367)
                     /locus_tag="PKH07_14140"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNT36121.1"
                     /translation="MTDRKYLVYYLSILAFATAFSIFPTTVTTLAREFQVAPERLDWL
                     SSTISGGFFLAMIFCTCLSERIGKDRLVWAGLVFLFLALFSATQVVRLPLLAVPALFL
                     GFGAGLIEISATALISEMTE"
     gene            complement(439..1191)
                     /locus_tag="PKH07_14145"
     CDS             complement(439..1191)
                     /locus_tag="PKH07_14145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HNT36122.1"
                     /translation="MAKIPIALQLYSVRDDCAKDFPATVAAVAKMGYVGVEFAGYYGR
                     TAPELRKMLDDLGLKCCGTHIGLDTLMGDTLKATIEFNQILGNKFLIVPGLAEQYRNS
                     RQAWVNTAKIFNDLVAQVKDQGMYVGYHNHMIEFQPMDGELPWDSFFGNTVKEVVMQF
                     DTGNALHGGAAATPFIKKYPGRALTVHLKEFSSTNDVAVIGEGDVDWKEVFQACETVG
                     GTQWYIVEQERYGVAPLVCVEKCLQALRAMGK"
     gene            1538..>1799
                     /locus_tag="PKH07_14150"
     CDS             1538..>1799
                     /locus_tag="PKH07_14150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36123.1"
                     /translation="MQKLKFARFLMLVCCFAAVHAGWCQEPSGEISPMAQVGAEGLTF
                     WQMIRYGGLTMFFIGLLSVFACLLVIYQALTLSSDKILQPGMV"
BASE COUNT          415 a          475 c          506 g          403 t
ORIGIN      
        1 cttcggtcat ctcggagatg agagccgtgg cagagatttc gatcagtccg gcaccaaagc
       61 ccagaaacag agcgggaaca gccagcaggg gcagtcggac aacctgtgtc gcgctaaaga
      121 gcgccagaaa taagaacacc agacctgccc atacaagacg atccttaccg attctttcgg
      181 agaggcatgt gcagaagatc atggcaagaa agaagccacc cgagatggtt gaggaaagcc
      241 agtccaagcg ctctggcgcc acctggaatt cacgtgccag cgtggtgact gttgtgggga
      301 aaatactgaa cgctgtcgca aaggcgagga tggagagata gtagacgaga tactttcgat
      361 cggtcacaga tatgtctcag accagaagcc cccggctcgt gccaggggct ttggctgcat
      421 caacctttgc accgttcgct acttgcccat ggcacgcagc gcctgaaggc acttttcgac
      481 gcacaccaga ggggcaacac cgtagcgttc ctgctcaacg atgtaccatt gggttccgcc
      541 aacggtctca caggcctgga agacttcctt ccagtccaca tcgccttcac caatcacggc
      601 aacgtcattg gtcgaagaga actctttcag atggacggtc agcgcacgtc caggatattt
      661 cttgataaac ggcgttgcgg cagcaccccc gtgaagcgca ttgcctgtgt cgaactgcat
      721 gacgacctct ttgaccgtgt tgccgaagaa ggagtcccaa ggcagttcgc catccatcgg
      781 ctgaaactcg atcatgtggt tgtgatagcc aacatacatt ccctggtctt tgacctgagc
      841 gacgagatca ttgaagatct tggcggtgtt tacccacgcc tggcgggagt tgcggtattg
      901 ctcggcaaga cccggaacga tcaggaactt gttgccgaga atctgattga actcgatggt
      961 cgccttcagg gtgtcaccca tcagggtatc caatccgatg tgtgttccgc agcacttcag
     1021 cccgaggtca tcgagcattt tgcggagttc cggagccgtc cgcccatagt acccggcgaa
     1081 ctccacaccg acatagccca ttttggcaac cgcagcgact gtcgccggaa agtccttcgc
     1141 gcagtcgtcg cgcacagagt aaagctgcaa tgcaatcgga atcttagcca ttaccttgtt
     1201 cctcacggtc aaaaagatga tgcgatcaca aagaccgctg ctgaaaagct atagtgaaac
     1261 gccggatgcc tgtcaatgga gaaacggatg gaggccgatg gttgttggat cggctgagtc
     1321 aaatgtaaaa cctgccccaa aatccgttga gagtgacacc gctggtgaca cattattggg
     1381 ggcgtgttga atttgacttt tctccggtgc gtccgtacag ttgatagcgg taagagacgt
     1441 tttcgagagc ccttgcaaac cgagcgcaag ggggtattct tgggaaatgc ccgtcggctc
     1501 gggaaatgcg ggttttggga ggcaggagtc catcacaatg cagaaattga agttcgctcg
     1561 tttcctcatg ctggtttgtt gttttgcagc cgttcacgcc gggtggtgcc aggaaccatc
     1621 gggtgagatc tcgccgatgg cacaagtcgg tgccgagggt ctcacgttct ggcagatgat
     1681 ccgatacggt ggtttgacca tgtttttcat cggtcttctg tccgtgtttg cctgtctgct
     1741 ggtcatctac caagctttga cgctgagttc ggacaagatt cttcagcccg gcatggttc
//
LOCUS       DAOQUC010000930         1799 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_85999_length_1799_cov_2.944954, whole genome shotgun sequence.
ACCESSION   DAOQUC010000930 DAOQUC010000000
VERSION     DAOQUC010000930.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1799
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85999_length_1799_cov_2.944954"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /locus_tag="PKH07_14155"
     CDS             complement(<1..500)
                     /locus_tag="PKH07_14155"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HNT36124.1"
                     /translation="MLKSLIGHTASKALLPVSQVAYRLRITPNQITVVGMLLTLIVGV
                     FIWQGHWLSAGLVLLLSGLLDLLDGSLARNFRMETRFGAFLDSTCDRISDAAIHLGLA
                     FYYAQNETGLLWPFLCCIAFLGAFMTSYARARAECLILRCDVGLAERPERIVLILAGL
                     LFRVMKP"
     gene            702..1013
                     /locus_tag="PKH07_14160"
     CDS             702..1013
                     /locus_tag="PKH07_14160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36125.1"
                     /translation="MTQVLTLVLLAAILCLFFVKRERVRWRIFLETSLLVLVITMCFD
                     LSSETVALEDYARPAIIVLLFMLLYLRYQAWVKRAKSLKPPETNQETQPQPVPTESPS
                     R"
     gene            1464..>1799
                     /locus_tag="PKH07_14165"
     CDS             1464..>1799
                     /locus_tag="PKH07_14165"
                     /inference="COORDINATES: protein motif:HMM:NF014628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HNT36126.1"
                     /translation="MVSSFCGKFESSAKLNGVNVAHCITTSDVVQYLLRLAHRNGTDR
                     FLSVPPPFAEEKIILEGLRREGMKCFQEDIRKWILQPCFGLTGAVSGIADTGALLLNV
                     ANESTRLVSS"
BASE COUNT          418 a          457 c          482 g          442 t
ORIGIN      
        1 ggtttcatca cccggaacaa cagaccagcc aaaatcagca cgattctctc cggccgctcc
       61 gcgagaccaa cgtcacagcg cagaatcagg cattccgccc gtgctctggc gtaactggtc
      121 atgaaggctc ccagaaaagc gatacagcaa agaaacggcc agagaagtcc tgtctcgttt
      181 tgagcgtagt agaacgcaag cccgaggtga atagccgcat cggagattcg atcgcaggtt
      241 gagtcaagga acgctccgaa acgggtctcc attctgaaat tgcgcgcaag ggaaccatcc
      301 agaagatcca ggagtcctga gagcagaagc acaagacctg cactcagcca atgaccttgc
      361 cagatgaaca ctccgacaat caaagtcaga agcatgccga caaccgtgat ctgattcggc
      421 gtaatccgca ggcggtaggc cacttgggac acaggtaaga gggcttttga agcagtatga
      481 ccgatgagcg atttcagcat gacccatcca tgagtacgca ttcctcgatt ttgttgtagc
      541 cactcaagac tctccgtaag tcttgatcgg gagaagcctt ctcgtcaaag gtaaaacagt
      601 ctcaacgatc aaaacagttg cccattccaa ccgctcggcg tattatctca gatagcgggt
      661 gcatgtgctg gccgcatgac tatcgcccgg gaggttacat tatgacccaa gttttgacgc
      721 ttgttcttct agccgccatc ctttgtcttt tctttgtcaa gagggaacgg gttcgttggc
      781 ggatcttcct cgagacgtcg cttcttgtcc tggtgatcac catgtgtttt gatctttcat
      841 ccgagacggt ggctttggag gactacgcga gaccagcgat catcgtactt ttgtttatgc
      901 ttctctattt gcggtaccag gcgtgggtca agcgagccaa gtcgctgaag ccgcctgaga
      961 cgaatcaaga gactcagcca caaccggtgc caactgaatc tccgtctcga taacgcgttg
     1021 agcccttcaa ctcatgttgc ggtgttttcc gtcttccagt ggtagtgttc ttgaactgtg
     1081 cgcgtaaggc cgagtggcag atatgctctc cggcgacggt tcgaaaactc aatgtcctct
     1141 gtgtttccag tatgtgaagg tgcagtatct ttggtcggca ggatctcaca catattccgt
     1201 tccggtctct ctcgagcgtt ctgtctcacg aagggatgac cgccgctttt ccgaacctgt
     1261 gaacaagcga gttgatctgc ttcggtactc tgtttgacac gagtgtctga atccgttagt
     1321 agtattctgg ttttcatgca gcgggatgct tcaagtggtt caaagcgtct ttcgaagacc
     1381 agcatccgaa gaagtttgag caagtttaca gcccaggtga tctgttgcgg aaacaggctt
     1441 tcggacagga aatgatctaa ggtatggtgt cttctttttg cggaaaattc gagagtagtg
     1501 cgaaactcaa cggtgtcaat gtcgcgcact gcatcacgac gagtgatgtt gtccagtacc
     1561 tgttgcggtt ggcacatcgc aacggtaccg acaggttcct ttccgttccc ccgccatttg
     1621 cggaggagaa gatcatcctc gaaggacttc gccgcgaagg catgaagtgt ttccaagagg
     1681 acattcggaa atggattctg cagccatgct ttgggctcac gggagccgtt agtggcattg
     1741 ccgacacggg ggcgctgttg ctcaatgtgg cgaatgagag cacacggttg gtgagttca
//
LOCUS       DAOQUC010000931         1794 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_86378_length_1794_cov_2.828637, whole genome shotgun sequence.
ACCESSION   DAOQUC010000931 DAOQUC010000000
VERSION     DAOQUC010000931.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1794
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86378_length_1794_cov_2.828637"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..421)
                     /gene="smpB"
                     /locus_tag="PKH07_14170"
     CDS             complement(<1..421)
                     /gene="smpB"
                     /locus_tag="PKH07_14170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HNT36127.1"
                     /translation="MAEKYYKLICSNKRARHDYHLLETFEAGIALKGTEVKSLRAGKG
                     SIKESYASFDNSELYLVDMHIPPYDHGNVMNHDPYRPRKLLLHAQEMRRLIGKVVERG
                     LTLVPTRMYFKGDNVKVEIALAQGKKLHDKREDLKRDE"
     gene            complement(430..1416)
                     /gene="argF"
                     /locus_tag="PKH07_14175"
     CDS             complement(430..1416)
                     /gene="argF"
                     /locus_tag="PKH07_14175"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013748835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HNT36128.1"
                     /translation="MTDREILRKVAATEPGGKTVKGYDIISIEDLNDQQIQEVLSLAA
                     QMKAAPERYRHTLAGRSMCMLFEKPSLRTRVTFEVGIASLGGYAVYMGPTDVALGKRE
                     AVEDVARNLDRWVDLIVARVFAHRTLETLAKYSQAPVINALSDIEHPCQALADIMTIF
                     EHKGRFAGVKCVFIGDANNVCNSLFLVGSRLGMKLAQGGPKDYQMNADLLKTIQANAS
                     KSGGSITICEDPIEAIRDADVVYTDIWTSMGQESQKHNKDRAFEGYQVNTDLMVHAKP
                     DAIFMHCLPAHRGEEVTSEVLDSPQSVVFDQAENRLHAQKALILKVLGGDIG"
     gene            1615..>1794
                     /locus_tag="PKH07_14180"
     CDS             1615..>1794
                     /locus_tag="PKH07_14180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36129.1"
                     /translation="MVLYPCLKLWTSVLFALCLLIAMAETSCAEARVAALFRSGFPDE
                     DVALARSLKKRLDGEG"
BASE COUNT          370 a          467 c          475 g          482 t
ORIGIN      
        1 gttcgtcgcg cttgaggtct tcccgcttat catggagttt cttaccctgc gccagagcga
       61 tttccacttt cacgttgtcc cctttgaagt acattcgagt tggaaccagt gtcagccctc
      121 gttcaacaac ttttccgatg agtcgccgca tctcctgtgc atggagaagc agttttctcg
      181 ggcgataggg atcgtggttc atgacattgc catgatcgta cggggggata tgcatgtcca
      241 ccaggtacag ctcgctgttg tcaaaggaag cgtaactttc cttgatgctg cccttgccag
      301 cacgaagtga tttgacctct gtgcctttga gcgcaatgcc tgcttcaaag gtctccaaca
      361 ggtgatagtc gtggcgggca cgtttgtttg agcagatcaa cttgtagtat ttctctgcca
      421 ttctgctcct caaccaatgt cgccacccag aaccttcagg atgagcgcct tctgggcatg
      481 aagacgattc tccgcctgat cgaagacaac ggactgcggg ctgtccagaa cttcgctggt
      541 cacctcttcg ccgcggtgag cgggaaggca gtgcatgaag atggcatctg gtttggcatg
      601 taccatcagg tcggtgttga cctgatagcc ttcgaaagcc ctgtccttat tgtgcttctg
      661 agactcttgc cccatgctcg tccagatgtc cgtgtagacg acatccgcgt cacgaatggc
      721 ctcaatggga tcttcacaaa ttgtgataga gccaccggac ttggacgcgt tggcctgaat
      781 tgtcttcaac agatcagcat tcatctgata gtccttcggt cccccctggg cgagtttcat
      841 gcccagtcgg gaaccaacga ggaacagtga gttacaaacg ttattcgcgt caccgatgaa
      901 aacacatttg acgccagcga atcgtccctt gtgctcaaaa atggtcatga tgtctgccaa
      961 agcctggcag ggatgttcga tatccgagag cgcattgatg actggggctt gtgagtactt
     1021 ggcaagcgtc tccagtgtgc gatgagcaaa aactcgggcg acaatgagat ccacccatcg
     1081 atcgaggttt cgcgccacgt cttcaacagc ctctcgtttg cccagggcaa catctgtcgg
     1141 acccatatag acggcgtagc caccaaggct ggcaatgccg acttcgaagg tgacgcgagt
     1201 tcgcagcgag ggcttttcaa aaagcataca catcgaacga cccgcgagcg tatggcgata
     1261 gcgttcgggt gctgccttca tctgtgctgc aaggctcagg acttcctgaa tctgttgatc
     1321 gttcaaatcc tctatagaga taatatcgta acctttcact gtcttgcctc ccggctctgt
     1381 tgctgcgacc tttcggagaa tctctctgtc tgtcattctc gcacccaaat ctcaggcgcg
     1441 atctagattg tccgaatccc tctcagagtt cctgaaggga aaaagaattt atagcacggg
     1501 agcaggtgca atgcaacgac tgctgagaag ttgctttggt gtacgctgct ttctgtgttg
     1561 acttcccatg tgtttttcgt attctgaagg cgatcatatc tgaggagggt tctcatggtt
     1621 ctgtatccct gtttgaaact ctggacatcg gttttgtttg ctctctgcct tctcattgct
     1681 atggcagaga cgagttgtgc cgaggcgcgt gttgccgcgc tctttcgctc cggttttccg
     1741 gatgaggacg ttgctcttgc gaggtcgctg aagaagcgtt tggatgggga gggt
//
LOCUS       DAOQUC010000932         1793 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_86464_length_1793_cov_2.899885, whole genome shotgun sequence.
ACCESSION   DAOQUC010000932 DAOQUC010000000
VERSION     DAOQUC010000932.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1793
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86464_length_1793_cov_2.899885"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..548
                     /locus_tag="PKH07_14185"
     CDS             <1..548
                     /locus_tag="PKH07_14185"
                     /inference="COORDINATES: protein motif:HMM:NF012998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prephenate dehydratase domain-containing
                     protein"
                     /protein_id="HNT36130.1"
                     /translation="LAQCRGWLTANLPKAELFEVASTGRGVEIARQEDKSAAVGSLMA
                     GELFNLPVVAEHISDKTENTTRFFVIGKKIAEPTGNDKTSLILFLSDRVGALSDALEP
                     FKREKINLSRIESRPTKRAAWEYMFYLDLLGHSDDAKVRLVFDQLKRDILMLKIIGSY
                     PIELVPEARCRPLPSPGAETT"
     gene            568..1425
                     /locus_tag="PKH07_14190"
     CDS             568..1425
                     /locus_tag="PKH07_14190"
                     /inference="COORDINATES: protein motif:HMM:NF014234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HNT36131.1"
                     /translation="MGFGTVGILGVGLLGGSLGMALKSAYLAERIIGIGRNQQRLQLA
                     LECGAVDEVSLNVSCVAPVLDLLVIATPVDRIASFVQESAEKLKPGAVITDVGSTKAK
                     VVAQSESSIGRDDVFFVGSHPMAGGEKTGVQAARKDLFEGATCFVTPSPSTNPGARDQ
                     VCQLWRSVGGNVHLVSPEEHDALVAASSHLPHLIASALCLSAERSAENHAMLSKALGA
                     GFKDTTRIAAGSEDLWRDICMHNRNNLLDCFEQFSLILEEIRIALGTNQEERLEALLR
                     RAREFRENL"
     gene            1458..>1793
                     /locus_tag="PKH07_14195"
     CDS             1458..>1793
                     /locus_tag="PKH07_14195"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012536424.1"
                     /note="catalyzes the formation of
                     5-O-(1-carboxyvinyl)-3-phosphoshikimate from
                     phosphoenolpyruvate and 3-phosphoshikimate in tryptophan
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HNT36132.1"
                     /translation="MKRLIVRPSSQPLKGTIEVPGDKSISHRSLLLGALAEGTTEITG
                     LLEGEDCLCALKAIEALGVAVDSVGKSHYRVHGVGLQGLQEPDSVLDMGNSGTGIRLL
                     AGVLAGQPFL"
BASE COUNT          383 a          470 c          537 g          403 t
ORIGIN      
        1 ccttggcgca gtgccgtggt tggctcacgg cgaatctccc aaaggcggag ttatttgaag
       61 ttgccagcac gggacgcggc gtcgaaattg cccgacagga agacaagtcc gctgcggtgg
      121 gcagccttat ggccggcgag cttttcaatt tgcccgttgt ggcggaacac atctccgaca
      181 agactgagaa cacaacgcgc tttttcgtca tcggcaagaa gattgccgaa cccaccggca
      241 acgacaagac ttcgctgatc ctgtttctgt ccgaccgtgt gggcgcgctc tccgacgctc
      301 tggaaccgtt caaacgcgag aagatcaacc tctcccgtat cgagtccaga cccaccaagc
      361 gcgctgcctg ggagtatatg ttctatctgg atcttctggg acattcggac gatgcgaagg
      421 ttcgtctggt ttttgatcag ttgaaaagag atatcttgat gttgaagatc atcggatctt
      481 atccgataga actcgtgcca gaagcccggt gtcggccgtt gccgtctccg ggggcagaga
      541 ccacctgagg agttcggacg aaagagtatg ggttttggaa cggtcgggat actgggcgtt
      601 ggcttgttgg gtggctcgct gggcatggcg ctcaagtcgg cttatctcgc agaacgaatc
      661 atcggaatcg gcaggaacca gcagaggctc caactggctc tggagtgcgg ggctgtggat
      721 gaggtttcgc tgaatgtttc ttgtgttgca ccggttctgg atctgcttgt catcgccaca
      781 ccggtagaca ggatcgcttc gtttgtccag gagtcagcgg agaaactcaa acccggtgcc
      841 gtgatcacag acgttggcag taccaaggcc aaggtcgtgg cgcagtccga atcaagtatt
      901 ggcagagatg atgtcttctt tgtcggctct catcccatgg caggcgggga gaagacaggc
      961 gtacaggcag cccgcaaaga tcttttcgag ggagcaacct gttttgtgac tccctctccc
     1021 agcacaaatc ccggagccag ggatcaagtc tgtcaactgt ggcgttccgt tggggggaac
     1081 gttcatctgg tttcacctga agaacacgat gctcttgtgg cggcctcaag tcacctgccc
     1141 catctcatcg ccagcgcgct gtgtctgtct gcagagcgca gcgctgagaa tcatgcaatg
     1201 ctatccaagg cgttaggtgc cggattcaag gatacaaccc gaatcgctgc cggttccgaa
     1261 gacctgtggc gggacatctg catgcacaac cgaaacaacc tgcttgactg tttcgagcag
     1321 ttcagtctga ttctcgaaga gatacggatc gcattgggaa ccaatcagga ggagaggctt
     1381 gaggcgctgc ttcgccgcgc acgggaattc cgtgaaaatc tctagacccg cgtgctttac
     1441 agagcctcgc aatgcgtatg aagagactta tcgttcgccc ctcgtcacag ccgttgaaag
     1501 gcaccattga agtcccagga gacaagtcca tctcccatcg ctcactgctt ctgggggcat
     1561 tggcggaagg aacgaccgag atcaccggtc ttcttgaagg agaggattgc ctctgcgcgc
     1621 tgaaggcaat cgaggcactg ggtgtggcgg tggactccgt tggcaaaagc cattaccgag
     1681 tacatggtgt ggggctccag ggacttcagg aacctgattc ggtattggac atgggaaact
     1741 cgggtacagg cattcgtctg ctggcagggg tacttgccgg tcagcctttt ctg
//
LOCUS       DAOQUC010000933         1793 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_86473_length_1793_cov_2.691600, whole genome shotgun sequence.
ACCESSION   DAOQUC010000933 DAOQUC010000000
VERSION     DAOQUC010000933.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1793
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86473_length_1793_cov_2.691600"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..712)
                     /locus_tag="PKH07_14200"
     CDS             complement(<1..712)
                     /locus_tag="PKH07_14200"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNT36133.1"
                     /translation="MRPFDLTELSLPKNSPILSFVKHCRDVQPVRFDEGDYLFNENEL
                     SRDVYLVLSGAYVIEQGRTPLDGRPTAILSTVMSEPDSPSFVGEMAYLGKVPRTASVR
                     SSGCTFALQLKPQHLDLIIEKFPFLTQILCRQFAERLRETNNQFRELQSLLAMNASHR
                     FAKPEEVLFRKGDKAELLYQLISGEAIREETGESLTPDNLPAGFIEPGCFLRGGQYPY
                     TVKSTMMSLVVVIKRDSHL"
     gene            complement(833..>1793)
                     /locus_tag="PKH07_14205"
     CDS             complement(833..>1793)
                     /locus_tag="PKH07_14205"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNT36134.1"
                     /translation="RNAAANRIIPGCDALPQVAGLDALCSSEIQALVDEVLSQQARPG
                     IASKELVFEKLTFLVNVSQVLEPSGEVSGAVAVLRDITPLKQLETAKTLFMSMVAHEF
                     KSPLYAVEGLLSSVVGGVGGPDPERDRHFIERSLLRIKTLQALVSDLLNLTAMETGRF
                     TVNRLPLDITPVLHEVTETCRDHAMDQKIDLSIVDLPADKPLFVLADREALTIVLTNL
                     VDNAIKYSPEGGQVRVCVDRDPSYTRVHIQDNGIGMSPEEQEHVFDEFFRAKNKKAIH
                     VPGTGLGLSLVKRLVDLHQGRISVRSAPDKGSTFTVSLPNCSY"
BASE COUNT          423 a          505 c          463 g          402 t
ORIGIN      
        1 caaggtgtga gtccctcttg atgaccacga cgagagacat catcgtggac ttgacagtgt
       61 atggatattg cccaccgcgc agaaaacacc ccggttcaat gaatcccgct ggaagattgt
      121 cgggtgtgag actctctccg gtttcctctc gaatcgcctc gccggaaatc agctgataga
      181 gcagttccgc tttgtccccc tttcggaaaa gcacttcttc cggcttggcg aaccggtgac
      241 tggcattcat cgccagaagg ctctgcaact cccgaaactg attgttggtt tcgcgaagtc
      301 tttccgcaaa ctgccgacag aggatctgag tgagaaacgg aaacttctcg atgatcaaat
      361 ccaagtgctg aggcttcagt tgcagcgcaa atgtacagcc agagctcctg accgatgccg
      421 ttctgggtac tttgcccaag tacgccatct cgcccacaaa agaaggtgag tccggttcac
      481 tcatgaccgt ggataggatg gcagtcggtc ttccgtccaa aggggttcgt ccctgttcga
      541 ttacgtaggc acccgagaga acaaggtaga catccctgga aagctcattc tcgttgaaca
      601 ggtagtcccc ttcatcaaaa cgcaccggct gtacatctcg acagtgtttg acaaaactca
      661 gaatcggtga attcttggga aggctcaatt ctgtcaggtc gaatggtctc atggtttgga
      721 ctccttgcct cgcaccattt caggacgacg gaagtgcgga gcaggccagc gcgctatgcc
      781 cactccctcg gacgcatgct cggtagatga cacgcctgtt ttgaaccaag tgtcagtagg
      841 aacagttcgg caggctgacc gtgaaggtgc tgcccttgtc cggagcgctc cttacggaga
      901 tgcgtccttg atgcaaatcc accaatcgct tcaccagact caaacccaag cccgtgcctg
      961 gaacatggat cgctttcttg ttcttggcgc ggaaaaattc gtcgaacacg tgctcctgtt
     1021 cttcaggaga catcccaata ccattgtctt gaatgtggac acgggtgtag gacggatctc
     1081 ggtcaacaca aactcgaacc tgtccacctt cgggagagta tttgatggcg ttgtccacca
     1141 gattcgtcag gacgatggtc agagcttccc tgtccgcaag cacaaaaaga ggcttgtctg
     1201 cgggcaaatc cacaatactc aggtcaatct tctgatccat ggcgtgatcc cggcaggtct
     1261 ccgtcacttc atggagaaca ggggttatgt ccagcggcag ccggttaacc gtgaatctgc
     1321 cggtctccat cgcagtcaga ttcagcaagt ccgaaacgag cgcctgaagg gtctttatcc
     1381 gcagtagaga gcgttcaatg aaatgtcggt cgcgttctgg atccgggccg cctacgcccc
     1441 ccacaacact ggaaagaagc ccttccacag catagagtgg actcttgaac tcatgtgcca
     1501 ccatggacat gaacaaagtc ttggccgtct ccagctgttt caacggcgta atgtctcgaa
     1561 gaacggccac agcacccgag acttcaccag acggttccaa cacttgactg acattcacca
     1621 gaaaagtgag tttttcaaac acaagttctt tggacgcaat gcccggtcgc gcctgttggc
     1681 tgagcacctc gtcaaccaac gcctgaattt ccgaggaaca caaggcgtcc agtccagcaa
     1741 cctggggcag agcgtcacag ccagggatga ttcgattcgc cgcggcgttg cgc
//
LOCUS       DAOQUC010000934         1788 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_86876_length_1788_cov_3.086555, whole genome shotgun sequence.
ACCESSION   DAOQUC010000934 DAOQUC010000000
VERSION     DAOQUC010000934.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1788)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1788)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1788
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86876_length_1788_cov_3.086555"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..916)
                     /locus_tag="PKH07_14210"
     CDS             complement(<1..916)
                     /locus_tag="PKH07_14210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNT36135.1"
                     /translation="MNSMVLAWISLKRRPWSTFLAVVSLAIALAILARTAQFFHYLRQ
                     GLTAYDHTVQVVVAPKSSSLEILLQALYGIGESTSVLPFALVTEAIQKQGTTEQVTPM
                     ALFGYWQDYPIFATDDTFMQRPQGLSAPQIALGEWFRESGQVVLGSEVARRSEAQIGD
                     TISCAAPFFHRGSTQPIWSREMIVVGILEPTESGHDGRILGTLHDAFDMQRIAIPLKM
                     LREASNQQAVSWFMVWTKPHQEQALKRFIDEKTVGYTMITKDAVRQLQGLFDAAVKVG
                     QLVLTAVLTLGGLCLAILLNTRFESMIEE"
     gene            complement(913..1560)
                     /locus_tag="PKH07_14215"
     CDS             complement(913..1560)
                     /locus_tag="PKH07_14215"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNT36136.1"
                     /translation="MDITIESFKVHWNDAPSALLDIPNWSVQSGSRILLKGPSGSGKT
                     TLLHCISGLMRPTEGRILVGETNTVNLSESESCLWRRNSVSVVFQRLNLLRHLTALEN
                     VCLAARGVPNPKETARQALQKLDMVRHIDRIAEEMSLGEQQRVAVARAVAWKSDLILA
                     DEPTSSLDEANTELVARTLLETSEVCQCTLIVVSHDQRLQRHFPHVVDIEEVGRA"
BASE COUNT          412 a          460 c          463 g          453 t
ORIGIN      
        1 gttcctctat catgctttcg aagcgggtgt taagaagaat cgccaggcac agtccaccaa
       61 gtgtaagcac tgctgtcaga acgagctgtc ccactttgac tgcagcatcg aaaagtcctt
      121 gcagttgtct gacggcatcc tttgtaatca ttgtgtagcc aacggtcttc tcatcaatga
      181 accttttgag ggcttgctcc tgatggggtt tcgtccagac catgaaccag gatactgcct
      241 gttgattgct tgcttcacga agcatcttca aaggtatcgc aatacgctgc atgtcaaaag
      301 cgtcgtgcag ggtaccgagt atccttccgt cgtgtccact ttcagtcggc tcgagtatgc
      361 cgacgacaat catttctcga gaccagatcg gctgggtact cccccggtgg aagaatggtg
      421 cggcgcagga gattgtgtcc ccaatctgtg cttcacttcg acgcgccacc tcgcttccaa
      481 gaacaacctg gccggattcc cgaaaccatt ctcccagtgc aatctggggt gctgaaagac
      541 cctgcggacg ttgcatgaat gtatcatctg tcgcaaatat cggataatcc tgccagtacc
      601 caaagagagc catcggggtc acctgctcgg tagtcccctg tttctggatt gcttctgtga
      661 cgagggcaaa cggaagaaca ctcgtgctct caccgattcc atagagggct tggaggagaa
      721 tctcaagact actcgacttg ggcgcgacaa caacctgcac cgtatggtcg taggcagtca
      781 gaccctgtcg tagatagtga aaaaactgag cagtccgcgc aagaatcgcc aaagcgatgg
      841 ccaggctcac aacagcaaga aatgtagacc aaggccttcg tttcaaagat atccacgcga
      901 gaaccatgct gttcatgctc tgcccacctc ttcaatgtca acgacatgag ggaaatggcg
      961 ttgcagacgc tggtcgtggc tgaccacgat gagcgtgcat tgacatactt ctgatgtctc
     1021 gagaagagtt cgtgcgacaa gttcagtatt ggcttcgtca aggcttgagg tcggttcatc
     1081 tgcgaggatg agatctgact tccaggccac agcacgagcc accgcaacac gctgttgttc
     1141 tccgaggctc atctcttcgg cgattcggtc aatgtggcga accatgtcca gtttctggag
     1201 agcctgtcgc gcagtttctt tgggattggg gactccacgg gcggcaaggc agacattctc
     1261 cagggcagtt aggtgccgca gcagattcag tcgctggaaa acaacactga ctgaattcct
     1321 tcgccatagg caagattcag actcgctaag gttgaccgtg ttcgtttctc caacgagaat
     1381 ccttccctct gtcggcctca tgagtccgga tatgcaatga agtaatgtgg tcttgccact
     1441 tccacttgga ccttttagaa gaattctgga acctgactga acgctccagt ttggaatgtc
     1501 gaggagggcg ctcggcgcat cgttccagtg aaccttgaaa gactcaatag taatatccat
     1561 gtttcgtggt ctcagtgtga attgtttgtc tttctgtttg gttgtttgtc cgtgagaaag
     1621 gctcagcatt gccattcttg gacttccgac gtttggccgg tggttcacct actctttttg
     1681 gaacgaccca acctgaagag aggttacggt aagtttcata atggtcagat gcgcttcgca
     1741 gccgaaaagt ccttagtgaa gagcaatctc attggttact tcggactg
//
LOCUS       DAOQUC010000935         1787 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_87012_length_1787_cov_2.271940, whole genome shotgun sequence.
ACCESSION   DAOQUC010000935 DAOQUC010000000
VERSION     DAOQUC010000935.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1787
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87012_length_1787_cov_2.271940"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..727
                     /locus_tag="PKH07_14220"
     CDS             <1..727
                     /locus_tag="PKH07_14220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36137.1"
                     /translation="SVLFQQENAVSSQGTTPQEQLLQLIIGSIFHELLRLKQYLYVLE
                     THQAQMAMLEETLEARYFLLRHGKALVREAEMKLPSLMEELRELWEEANTFLHQWLPQ
                     FAENRVILRFLFRNQILIDRAYGYRGFENVLSYLFEHGAKDGYYRMGMDYYRSSHFAE
                     AVDCFGRLLDLYENNKSNQSSALADSPKIPKTTRAKRLREILKSSKNMVAFVTGKPGM
                     GTELAPFSNLVLRVEALLDLVAG"
     gene            1061..>1787
                     /locus_tag="PKH07_14225"
     CDS             1061..>1787
                     /locus_tag="PKH07_14225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36138.1"
                     /translation="MKYSILSLVLCSLFVCGSAFAGGDWTDWFDDYANQSALEANWTA
                     VSGFASGFLDNSRVLSATQSMKIEKGRKPTRALNTQGRFTIWVYDDGATPKQFDVEVH
                     SKGGTPGFVALGLRDDGTSGNDQYTCNINGTVTKTGIPRTTGWHLFQYQINNADKGGW
                     KVKLDGKGFPGNVPSLTDGDKVIINTEFGASGDTNTIWVDSARYNHNANADRLLSAVD
                     ETYILDYGFEKDVTNWTALPGTVN"
BASE COUNT          453 a          421 c          485 g          428 t
ORIGIN      
        1 cagcgttctt ttccagcagg aaaacgctgt atccagtcag ggaacaactc ctcaggaaca
       61 gctgctgcaa ctgatcattg gctcgatctt ccacgaactg ctccgactga agcagtacct
      121 ttacgttctg gaaacgcatc aggcgcagat ggccatgctg gaagaaacct tagaggcacg
      181 gtactttctt ctgcgtcatg gcaaagcgct ggttcgtgaa gcggagatga aactgccgag
      241 cctgatggag gaactccgag aactctggga agaggccaac acctttctgc accagtggct
      301 gccccagttt gccgagaaca gggtgattct cagattcctt ttccgcaacc agatcctcat
      361 tgaccgagcc tacggttacc gcggctttga gaatgtgctg agttaccttt ttgagcacgg
      421 tgctaaagac ggatactatc gtatggggat ggattactat cgttccagcc acttcgcaga
      481 ggccgtagac tgctttggca ggctgttgga tctctacgag aacaacaaga gcaatcaatc
      541 ctctgcactt gcagatagtc cgaaaattcc aaagaccacc cgcgccaaac gccttcgtga
      601 gatcctgaaa tcttcaaaga atatggttgc ttttgtgact ggcaaacctg ggatgggtac
      661 cgaactggcg cctttctcca atcttgttct ccgtgtcgag gctcttctgg atttggtggc
      721 cgggtagttt gttgataccg gcagagccaa ccaccgactc ggttcttatc ctaccaccca
      781 tagtacaggc acccagcccg ccagccatgg gcagactcat gcgggcaaga tggcctcatc
      841 acaaacgaag ccgacagtga ccaagatttt gagaattggg aagaatgttg acagaagtag
      901 cgcatggatg tacttttacg aagaacgagg acagtatggg atattactgt tagggagtcg
      961 ggaggtggta aaacgaggca ttcttttgaa gctctcaacg taccaagggc aactgtgccg
     1021 ttgtctttgg ggatagtctt gaatcaacgg aggagatgaa atgaagtatt cgatcttgtc
     1081 attagttctt tgttctttat ttgtttgtgg aagcgctttc gcaggaggag attggacaga
     1141 ctggtttgac gactatgcta accaatctgc gctggaagcg aactggacag ccgtaagtgg
     1201 ttttgccagc ggctttctgg ataactcacg tgtcctttcg gcaactcagt ccatgaagat
     1261 tgaaaagggg cgaaagccga ctcgtgcctt gaacactcaa ggtcgtttta ctatctgggt
     1321 ctatgatgat ggggcaacac ccaagcagtt tgatgtcgaa gttcattcga aaggtggaac
     1381 acctgggttt gtggcacttg gtttgagaga tgacgggaca agcggcaatg accagtacac
     1441 ctgcaatatc aatggcacgg tgaccaagac cggtattccc cgcacgacag ggtggcatct
     1501 gttccaatac caaatcaaca atgcggataa gggtggctgg aaagtaaaac tcgacgggaa
     1561 gggctttccg ggaaacgtcc cgtcgctgac ggatggtgac aaggtcatta tcaacactga
     1621 gtttggcgca tcaggcgaca ccaacacaat ttgggtggac agtgcgcgct acaaccataa
     1681 cgccaatgct gatcggctgt tgtcagcggt tgatgagaca tacattctgg actacggatt
     1741 cgagaaggat gtgaccaatt ggacggctct cccgggtact gtgaata
//
LOCUS       DAOQUC010000936         1786 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_87079_length_1786_cov_2.813980, whole genome shotgun sequence.
ACCESSION   DAOQUC010000936 DAOQUC010000000
VERSION     DAOQUC010000936.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1786
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87079_length_1786_cov_2.813980"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1200)
                     /gene="lysS"
                     /locus_tag="PKH07_14230"
     CDS             complement(<1..1200)
                     /gene="lysS"
                     /locus_tag="PKH07_14230"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0017101 - aminoacyl-tRNA synthetase
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HNT36139.1"
                     /translation="MAKHWAVELGEKIIARRGNDHVVSIGVSPSGPIHVGFLREIVIG
                     DVIRRTLSQMGANAKLVLTSDDMDPLRHVYPFLPKSYSENVGQPLCRIPDPEGCCPSY
                     AEHFIRPLEKAFVRLGVEIEVRRSSEKYDAGLFTDTVKQSLEKRQRIVDIIREESGRE
                     LGEDWTTYNAICSTCGRLTTTTVQECDVANHRIRYQCQNGHEGWADYSKAEGKLPWRI
                     DWAARWHVYGVTVEPFGKDHAGAGGSYDTGSRFSREIFDSEPPEPIPYEWIELRGLGP
                     MSSSKGVVITITDILEAVPAQLMRYLLVRTRASKHITFDPCVGLLQLWDEYEKIAQAI
                     QTGDEAAVTEIDRANYDYSLVPDAENPATLGISFKHLATAVQLAGDSESVLRESLLRS
                     GYRQQCEN"
     gene            complement(1227..>1786)
                     /locus_tag="PKH07_14235"
     CDS             complement(1227..>1786)
                     /locus_tag="PKH07_14235"
                     /inference="COORDINATES: protein motif:HMM:NF014615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HNT36140.1"
                     /translation="GGLLEPYGDQKPEEAEEGRLGQECATPYGDRGGTIIETMTDLEE
                     MKLALKAARQATSLPVIVSMSFQNGSVPHRTLMGQKTTDCALALKDAGASVIGSNCGV
                     GSAELLEIVREMHSVLGDVPLIAEPNAGLPIIEGDKTVFPETPEEMASRTGDFVRAGV
                     RLLGGCCGTSPKHIRALRQALDQIS"
BASE COUNT          377 a          540 c          462 g          407 t
ORIGIN      
        1 gttttcgcat tgttgccgat atccggatcg caggaggctt tcccggagaa ccgattcgga
       61 gtctcccgcc agttggacgg ctgttgccag atgcttgaat gagataccca aggtagcagg
      121 gttttccgca tcgggaacga gtgaatagtc gtagttggca cggtcaatct ctgtcacggc
      181 cgcctcgtca cccgtctgga tcgcctgagc gatcttctca tattcatccc acaattgaag
      241 caacccgaca cagggatcga aggtgatatg cttcgaggcg cgagtccgca ccagcaggta
      301 tcgcatcaac tgagccggaa ccgcctcaag aatgtccgtg atggtgatga caacgccctt
      361 ggaactggac attgggccca gtcctcgaag ttcaatccat tcgtaaggaa tcggctctgg
      421 tggttcagag tcgaatatct ctcgcgagaa tcggcttccg gtgtcgtagg aaccacctgc
      481 accggcatgg tctttgccaa acggttccac cgtcactccg tagacgtgcc aacgcgctgc
      541 ccagtcaatg cgccagggaa gttttccctc cgctttcgag tagtcagccc aaccttcatg
      601 accattctgg cactggtatc gaattcgatg gtttgccaca tcacattcct gaacggttgt
      661 ggtggtcagt cggccacagg tgctgcaaat ggcgttgtag gtcgtccagt cttcgcccag
      721 ttcgcgacct gactcttctc tgatgatatc cacaatccgt tggcgctttt ccagggactg
      781 tttgactgta tcggtgaaca agcccgcgtc atacttctcg ctggaacgcc gaacctcgat
      841 ctccactccc agacggacga acgccttctc gagtggtcgg atgaaatgct cggcgtaact
      901 cggacagcac ccttccggat caggaattcg gcacagaggc tgcccgacat tctccgagta
      961 actctttggc agaaatggat agacatgccg caagggatcc atgtcatcag aagtcagaac
     1021 caacttggcg tttgcaccca tttgactcag cgttcgtcga atgacgtcac cgatgacgat
     1081 ctcccgcaga aatccaacgt gaattggccc tgaaggactg acaccgatgg agacgacatg
     1141 gtcattgccc cgcctcgcga tgatcttctc tccaagttcg acagcccagt gttttgccat
     1201 ggttgctctc tcagtctcga tattgtctag gatatttggt cgagcgcctg acgcagcgcc
     1261 cgaatatgtt tcggacttgt gccacaacag ccacccagaa gacgcacgcc cgcacgcacg
     1321 aagtccccag ttcgactggc catctcctca ggcgtttctg gaaacaccgt cttgtcacct
     1381 tcaatgattg gcaggccggc gttgggctcg gcaataaggg gaacgtcccc caagaccgaa
     1441 tgcatctctc ggacgatctc caaaagctct gctgagccaa caccacagtt ggagccgatg
     1501 acgctggcac cggcgtcctt cagtgccaaa gcgcagtcgg tagtcttctg tcccatcaga
     1561 gtgcggtggg gcaccgaacc attctgaaaa ctcatggaca cgatgacggg gagtgaagtc
     1621 gcttgtcgag ccgccttcag cgccagtttc atctcttcca gatcggtcat tgtctcgatg
     1681 atcgtgccgc cccgatcccc atatggcgtc gcgcactcct gccccaaacg cccctcttcc
     1741 gcctcttcgg gcttctggtc accataaggt tcgagcaacc caccgc
//
LOCUS       DAOQUC010000937         1785 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_87145_length_1785_cov_3.419653, whole genome shotgun sequence.
ACCESSION   DAOQUC010000937 DAOQUC010000000
VERSION     DAOQUC010000937.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1785)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1785)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1785
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87145_length_1785_cov_3.419653"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1438
                     /locus_tag="PKH07_14240"
     CDS             <1..1438
                     /locus_tag="PKH07_14240"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNT36141.1"
                     /translation="LLGATDGYQVGDEMLKTIQTSSLLAIPLVTQERVIGQMIIDYEG
                     SPIPMGLAKMALENLAVVAASALERSVLMERASTQTESLARLSRQIEETQRQLQHSER
                     LASIGTLAAGAAHEINNPLAVISAKAQIMQRKATEQDQVEGYRIIIEQTKRIAKIIAD
                     LMGFARPTDPQAEPVSIKCVVERSLALVENRIALADIVVNTDFPDNLPLILGDPNQLD
                     QVLLNLFVNAQQAMQKGGEITVKADQSEDGKFIRVRFSDTGRGIPQKHLHRIFDPFFT
                     TKKQGEGMGLGLAVSHKIIETHGGQMSVESTVGVGTTFTLMLPISQSAQIREIQSELL
                     KSTPTTIQRSADNKGRILIVDDESHIRETLEGVLKEEGYEVDNACDGVEGLQKILDGN
                     VDLVLLDIRMPHRDGLEVLEGIKESFRRVPVIIVTGLAAEKEIKECLDAGAIECIRKP
                     FVINEVLQSVRKALKESRRQNKQGMRPA"
BASE COUNT          453 a          438 c          538 g          356 t
ORIGIN      
        1 actgctgggg gcgacagacg gctatcaggt gggggatgag atgctgaaaa caattcagac
       61 atcctccctg ctcgcgattc ccttggtgac ccaggaacgg gtcatcggtc agatgatcat
      121 tgactatgaa ggttcaccca ttccgatggg tttggccaag atggctctgg agaatctggc
      181 ggtggtggcc gcttcagctc tggagcgatc cgtcttgatg gagcgtgctt cgacccagac
      241 ggaatccctg gctcgactca gtcgccagat cgaggaaacg caacggcagt tgcagcattc
      301 ggagcgtttg gcttccatcg gaactctggc cgcgggtgcc gcgcatgaaa tcaacaatcc
      361 cttggcagtc atctctgcca aggcacagat catgcagcgc aaagcaaccg aacaggatca
      421 ggttgaaggc tatcgaatca tcattgaaca gaccaagcgc atcgcgaaga taatcgctga
      481 tttgatgggc tttgcgcgtc caacagatcc gcaggcggaa cccgtgtcga tcaagtgcgt
      541 tgtggagcgg tcgctggctc tggtcgaaaa ccgcattgcc ttggcggata tcgtggtcaa
      601 cacggacttc cccgacaatc tgcccctgat tctgggggat cccaaccaac tcgatcaagt
      661 cctgctgaat ctttttgtca atgctcagca ggcaatgcaa aagggcggtg agatcacggt
      721 aaaggcagat cagagcgagg acggcaagtt cattcgagtc cgtttctcgg acacgggacg
      781 aggcataccc caaaaacatc tccatcgcat ctttgacccg tttttcacaa cgaaaaaaca
      841 gggtgaaggt atgggactcg ggctggccgt atcacacaaa atcatcgaaa cccacggggg
      901 acagatgtcc gtcgaaagca cagtaggtgt tggaacgaca ttcacgctga tgcttccgat
      961 cagccagtcg gcgcagattc gcgaaatcca gtcggagttg ctcaagagca ctccgacgac
     1021 catacagcgc tctgctgaca acaagggacg cattctcatt gtggatgatg agtcccacat
     1081 tcgcgagacg cttgaagggg ttttgaaaga agaaggttat gaggttgaca acgcctgtga
     1141 tggtgtggaa ggcttacaga agattcttga tgggaacgta gacctcgttc tgcttgacat
     1201 tcggatgccc catcgtgatg gactcgaagt cctggaagga atcaaggaaa gtttcaggcg
     1261 ggtgcctgtc atcatcgtga cggggctcgc cgcggagaaa gagatcaaag agtgtctgga
     1321 cgccggcgcc attgaatgca tccgcaagcc tttcgtgatc aacgaagtgc ttcagtcggt
     1381 tcgcaaggca ttgaaagaat cccggcgtca gaacaagcag gggatgcgcc ctgcgtgaga
     1441 tgaagcgaat cattgacggc cttggggaac aggagagaga ttcgcaaccg gtgtctggga
     1501 gaaggtgctc acggtggagt acctgtcaac gagcaggaat gaatccggaa atggattcgg
     1561 cgatgaagtc gaaaccaagt gccggttggt gctgaagaca gagtcgagtg cccaagggac
     1621 ggggcgagct cggcacggag atccgaacgg tggaagatcg aaaatcaacc aaggggcgga
     1681 gccatctgac tctgcgtgaa gtggctaagg aagccggact ggaagagagc caagtccggt
     1741 actacgagac actttttggg gacatgcttc ccgaaaaagt ccaga
//
LOCUS       DAOQUC010000938         1785 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_87159_length_1785_cov_2.876301, whole genome shotgun sequence.
ACCESSION   DAOQUC010000938 DAOQUC010000000
VERSION     DAOQUC010000938.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1785)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1785)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1785
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87159_length_1785_cov_2.876301"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /locus_tag="PKH07_14245"
     CDS             <1..384
                     /locus_tag="PKH07_14245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 family protein"
                     /protein_id="HNT36142.1"
                     /translation="SILTGHNSPLMHAAASLVLNAAKHLAGLPEKLDLLPQNVMNSLQ
                     HLKKEILKGKMVSLDVEETLIALSISAISNPAAQMAVEHLKEMDGCEVHLTHIPTPGD
                     EAGLRKLGVNLTSDPDFSSKSLFIR"
     gene            537..>1422
                     /locus_tag="PKH07_14250"
     CDS             537..>1422
                     /locus_tag="PKH07_14250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36143.1"
                     /translation="MCVSPWRSRTVTGCIAALALHLMLFSLCESATIQGVQSGILEIA
                     SNGAFSVPINAIDPTKSFLIFQIRHNSDRPGGSLVGGRISSPTLLEFVRTTEELSPGS
                     IAVQWYVATFSSGVSVQRGELQQSSKTVNTSLTKLISLSQSFVLWSKVSPPKDVFWDD
                     NDPVFGELVSTNNLRFRSNAGGINNVIWWQVVEFTNPADISVQRARYSFGSTQTMYQA
                     PLAEPVNLASTFALLGYRVSGTIPGIGARIFRGYLPNATSLLVDRAQAGERGEDAEVC
                     WQIVHLKDSTRVQSGTVHF"
     assembly_gap    1423..1432
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1452..>1785
                     /locus_tag="PKH07_14255"
     CDS             1452..>1785
                     /locus_tag="PKH07_14255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36144.1"
                     /translation="MLTSSVDLSKTLAFSSVQSGGGQNMGKSPFATDDIIGVGCATLR
                     LADDDTLILERGNSSAEADIAWFVVEFGSDATATPTQTPTWTHTPTNTPTQTPTWTPT
                     ATPTFTDTN"
BASE COUNT          401 a          455 c          465 g          454 t
ORIGIN      
        1 agcattctca caggacacaa ttctcccctg atgcatgccg ccgcgagtct cgtgctcaat
       61 gccgccaagc accttgcagg tcttcctgaa aagctggatc tactcccaca gaatgtcatg
      121 aattcactcc agcatctcaa gaaggagatt ctgaaaggaa agatggtcag tctggatgtt
      181 gaggaaaccc taatcgcgtt aagcattagc gccatctcca atccggccgc tcagatggct
      241 gtggaacact tgaaagagat ggatggctgc gaggtgcatc tgacccacat cccaacgcct
      301 ggggatgagg ccggactgag gaaactgggg gtgaatctca cttctgaccc cgatttctcg
      361 tccaagagtc tctttattag atgagttttg ggaggcatgg acttccttga ttcggaaacc
      421 cgtccttcgg catccgaggg gatttccagg ggtagtggat attgttcgaa tacgatgcta
      481 tactcctaga aactatctgg tgcatctgtt gatgggcgga gttgaggaga tatggaatgt
      541 gcgtttctcc atggcgctcc cgaacagtta ccgggtgtat cgctgcttta gcactgcacc
      601 tgatgctgtt tagtttgtgt gaatcggcca cgattcaggg agttcagagt ggcatactgg
      661 agattgcatc caatggagca ttcagtgttc cgatcaatgc catagatcca acaaagtcct
      721 ttctcatttt tcagatcaga cacaatagtg accgtcccgg tggttccctt gttgggggac
      781 ggatatcctc acccacattg cttgagttcg taaggacaac cgaggaactg agcccgggtt
      841 cgatagctgt ccagtggtat gtggcaacgt tcagttcagg ggtatcggtt cagcgcggcg
      901 aactacagca gtcctcaaag acggtcaata cttctcttac aaaactcatc tctctgagcc
      961 agtcttttgt cctctggtcg aaggtctcgc cgcccaagga cgtcttctgg gatgataatg
     1021 accccgtttt tggtgagctt gtcagcacga acaacctgcg ctttcgttcc aatgctggcg
     1081 gaataaacaa tgtcatctgg tggcaggttg ttgaattcac caatccagcc gacatatctg
     1141 ttcagagggc tcggtattcc tttggctcga ctcaaaccat gtaccaagct cctctggcgg
     1201 agcctgtcaa tctggcgagt acctttgccc tgctgggcta tcgggtatct ggaaccatcc
     1261 ctggtattgg cgcaaggatt ttcagagggt atctgcccaa tgccacttcg cttcttgtgg
     1321 atagggcgca ggctggcgaa cgtggtgaag acgctgaggt ctgctggcag attgttcatc
     1381 tgaaggattc gactcgtgtt caatccggaa ctgttcattt ccnnnnnnnn nncagcgcgc
     1441 aggaaaatgt cgtgttgacc agttctgtgg atctttccaa gacgcttgcc ttttcatcgg
     1501 tgcagtcggg cggtggtcag aacatgggaa agagtccttt tgccaccgat gatatcattg
     1561 gcgtcggctg tgccactctt cgcctggcag acgacgacac gttgattctc gaacgcggaa
     1621 attcttctgc cgaagcagat attgcgtggt ttgttgtgga attcggcagc gatgcgacgg
     1681 ccacaccaac tcaaacgcca acttggactc atacgccgac aaacactcca acgcagactc
     1741 ccacttggac gcctacggca accccaacat ttaccgatac gaata
//
LOCUS       DAOQUC010000939         1781 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_87507_length_1781_cov_2.769409, whole genome shotgun sequence.
ACCESSION   DAOQUC010000939 DAOQUC010000000
VERSION     DAOQUC010000939.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1781)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1781)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1781
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87507_length_1781_cov_2.769409"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="PKH07_14260"
     CDS             <1..314
                     /locus_tag="PKH07_14260"
                     /inference="COORDINATES: protein motif:HMM:NF037386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36145.1"
                     /translation="PGHLPECEIQERLLHLTEQVELDCEKRKSRLLGMTVPVLVDGVC
                     GGEEDWYHGRWEGQAPDIDGHVYVQGKNLKPGCFYPVVLTHLVGSDYTGAFIREDESS
                     D"
     gene            complement(375..842)
                     /locus_tag="PKH07_14265"
     CDS             complement(375..842)
                     /locus_tag="PKH07_14265"
                     /inference="COORDINATES: protein motif:HMM:NF015999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HNT36146.1"
                     /translation="MPVQRVKDYLASQGIAFDSIVHERTYTAQETAAVTHVKGRELAK
                     AVVLNVDGECLIAVIPASRRLSLRKTKELLGANSLRLADESEFSRLFPGCELGAIPPL
                     GSLFGVKMLVDKTLREDEEIAFAAGTHTEIVRVKLSDFENLESPEYGELISGE"
     gene            1099..>1781
                     /locus_tag="PKH07_14270"
     CDS             1099..>1781
                     /locus_tag="PKH07_14270"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HNT36147.1"
                     /translation="MARGFSGHHGPPQMNDDDVKPLKITNARMLGWFYKNLKPHWHKV
                     LLGLVSMIAGTAAGLFVPMVLRSIFDDVIRDHKLDMLPGLIGKYLLFMLAGLVFSAVR
                     TNVMHLLGQRFVYKVRMDCYSNLLRLGLNYFESKRSGDIMSRISNDVEAVELMVVHGT
                     DDIISNTLHILGSVGFLFYLDWKMALVALAPLPLYVVCLILLARFIRPVFGKIRRELG
                     EINAKLQER"
BASE COUNT          363 a          467 c          508 g          443 t
ORIGIN      
        1 gtcccggtca tcttcccgag tgcgagatac aggaacgcct gttgcatttg acggagcagg
       61 tcgaactgga ctgcgaaaag cgcaagagca ggctcttggg gatgactgtg ccggtgctgg
      121 tggatggagt ctgtgggggc gaagaggact ggtatcacgg ccgatgggaa ggacaggcac
      181 cggatattga cggtcatgtc tatgtgcaag gcaagaatct gaagcccggc tgcttctacc
      241 ctgtcgtgtt gacacacttg gttggcagcg actacacagg tgctttcatt cgggaagacg
      301 agtcttcgga ctgaggcagt ctcgtcaccg cacgggggtg gagatcgccc gtgtagctac
      361 aggcgttggt ctgttcactc tcccgagatc agctcgccat attcgggtga ctcgaggttt
      421 tcaaagtcgg agagcttcac tcggacaatc tccgtgtgtg tgcccgcggc aaatgcaatc
      481 tcctcatctt ccctgagcgt cttgtctacc agcatcttca cgccaaaaag actgcccaag
      541 gggggtatcg caccgagttc gcagcccgga aacaagcgtg aaaactccga ctcgtcagcc
      601 agtcgaagac tgtttgctcc aagaagctcc ttggtctttc gaagactgag gcgtctggat
      661 gccggaatga cggcgataag gcattctccg tctacattga gcacgaccgc ctttgccagt
      721 tcacgacctt tgacgtgcgt caccgctgca gtctcctgcg ccgtgtaagt ccgctcgtga
      781 acgatggagt cgaacgcaat tccctgcgaa gcgagatagt ccttgactct ttgcactggc
      841 atggttgttc ctcctttgtc tcccaggaaa ccggattccg gagggtacgg tttccttctc
      901 gaaagagcgc cttgcggagt tgggcatatc ccagaagtcc gcaggcgctg gcctgcccaa
      961 atgcgatcca cattcataac gatacgttcc gtagcgctgt ccgtcaagcg agtgatcgat
     1021 gcgagtcgtt gtcggccttg ttgtcccacg cgaaacatcc tatactgcct ttcattgaac
     1081 taatggagga ttctgtcaat ggccagaggc ttcagcggcc accatgggcc gcctcagatg
     1141 aatgacgacg atgtcaagcc tttgaagata accaatgccc ggatgctggg ttggttctac
     1201 aagaatctca aaccgcattg gcacaaggtt ctgttggggc ttgtttccat gattgccggc
     1261 accgccgcgg gactctttgt ccccatggtg ctccgaagca ttttcgatga tgtgattcgg
     1321 gatcacaagt tggacatgct gcccgggctg attgggaaat acctgttgtt catgcttgcg
     1381 ggtttggtgt tttccgcggt tcgcaccaat gtgatgcatc tcttggggca gagatttgtc
     1441 tacaaggttc gcatggactg ctacagcaat ctcctgcgcc tgggcttgaa ctactttgag
     1501 agcaagcgtt ccggggacat catgtctcga atcagcaatg atgttgaagc cgtcgagctg
     1561 atggttgtcc atggcaccga cgacattatc agcaacacac tgcacattct ggggtcggtc
     1621 ggattcttgt tctatcttga ctggaagatg gcgcttgtgg cattggctcc actgcctctc
     1681 tatgtcgtgt gtctcatttt gcttgcgcgt ttcatccgtc cggtctttgg caaaatccga
     1741 agggagttgg gtgagatcaa cgccaaactc caggagcgca t
//
LOCUS       DAOQUC010000940         1781 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_87516_length_1781_cov_2.524334, whole genome shotgun sequence.
ACCESSION   DAOQUC010000940 DAOQUC010000000
VERSION     DAOQUC010000940.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1781)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1781)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1781
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87516_length_1781_cov_2.524334"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(214..1326)
                     /locus_tag="PKH07_14275"
     CDS             complement(214..1326)
                     /locus_tag="PKH07_14275"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HNT36148.1"
                     /translation="MNFSLKCSNHATGTSGTGDAGYICVCLEKKSELMRVTFLGRVLF
                     AFVAGLSLALLLVQQSHGETIRFKNGKILQGDVFDDEDDASMLKIVMGKGEMRVSRDL
                     VERIEDASDDETSIEDLTTPMVFGESSGLQIMDKKFQNASSASVNTTTDGRPTFQAQA
                     DPVTSSGGNSWAFGSGDGQIKPLLAKVVQVRGQVEILGEGSNWRALEPNETLSPGMQI
                     RTGNGRATLYTSDQVELRLTENAHLANLSETVIPTVRLESGKLWLKVHGVRDEKSGDP
                     RIVILAPNAIAGVRGSLLFLDAETSETTTIAVFDGEVAVLEPETGSELLTLSADQATR
                     IESPGQATSIRCESRWLEQWKFWGTWQTGAASEPSQ"
     gene            complement(1522..>1781)
                     /locus_tag="PKH07_14280"
     CDS             complement(1522..>1781)
                     /locus_tag="PKH07_14280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36149.1"
                     /translation="SAQMQVPMPIHGRVPDSHRRQVANKSRLFLSYGYHKRCSEKHLN
                     VLDKKKFPNAQKNLDFMIGWKYYTCGTIRIKEREADTDNPK"
BASE COUNT          416 a          498 c          423 g          444 t
ORIGIN      
        1 cgaacgggag tttcatagat gaaagcgctc cttgagttct ttctcctccg gcactgtatc
       61 tgacgcactg ggggtagttc tattcgaaca tcagggaaac tcatcaagaa atatcatccg
      121 aagcggagtt ccgacgcgcc atattcctcg ttgcaggtca gcgttagagg aaaacaacga
      181 cacccttgtc tgttccggat taccaagaga cgcttactgg ctgggttctg acgcagcgcc
      241 ggtctgccaa gtgccccaga atttccattg ttcaagccat cggctctcac atcgtatgga
      301 agtggcttga ccaggagatt ctattcgggt ggcttggtct gccgaaaggg taagcagttc
      361 ggaacccgtt tctggttcga gaacagcgac ctccccgtca aacacagcaa tggttgtggt
      421 ctcggaagtc tccgcatcga gaaacagcag agagccgcgc actccggcaa tagcattcgg
      481 tgccagaata acgatgcgcg ggtcgccgct cttctcatct cggactccat gcactttcag
      541 ccacagtttg cccgattcga gtcggactgt tggaatgact gtttcggaaa ggtttgcaag
      601 atgggcgttc tcggtcagtc tcaattcaac ctgatccgag gtgtagagag tcgcccttcc
      661 gttgcccgtg cgaatttgca ttcctgggga caacgtctca ttcggctcga gggctctcca
      721 gtttgatcct tcgccgagaa tttcgacctg tccccgaacc tgaaccacct ttgccagaag
      781 gggttttatc tgtccgtccc cggagccaaa cgcccaggaa ttgcctcctg aagaggtaac
      841 tgggtcggcc tgtgcctgga acgtgggacg gccatcagtg gtcgtattga cagatgcaga
      901 cgaggcattc tgaaacttct tgtccatgat ctgcaggccg gaggactctc cgaagaccat
      961 cggcgtggtc agatcttcaa tggaagtctc atcatcggaa gcgtcctcga tcctctcgac
     1021 aagatcgcgg gagactctca tctctccctt gcccatcaca atcttaagca tcgaggcgtc
     1081 atcctcgtca tcaaagacat ctccctgaag gatcttcccg ttcttgaagc ggatggtttc
     1141 tccgtgactt tgctgaacca agagcaatgc cagactcaaa ccagcaacaa acgcaaaaag
     1201 aactctcccg agaaatgtaa ctcgcatcaa ctcagatttc ttctcaagac agacacagat
     1261 ataaccggca tcaccggttc cagacgtacc agttgcgtga ttactgcatt tcaacgaaaa
     1321 attcaactct aatcgctcct ctactccctg aaaatcgcct cattcagagg tgtcttggag
     1381 tagagccatt caggtccccc gacatcagcc tccctctcgt tacccaaaag gcccttccaa
     1441 gcagccctta gcagctccgc ctcacggttc ctctactcgt gaagctccca gactcaattg
     1501 aaaccgaaca ctcgcgtcct actatttagg attatcggta tcggcctctc tttcctttat
     1561 acgtatcgtt ccgcaggtat agtacttcca cccaatcatg aaatcaagat tcttttgagc
     1621 attcgggaac tttttcttgt ctaatacgtt taagtgtttc tcagagcatc tcttatggta
     1681 accgtacgag aggaacaggc gagacttgtt ggcaacttgc cttctatggg aatcgggaac
     1741 gcggccatgg ataggcattg gaacctgcat ctgggctgat t
//
LOCUS       DAOQUC010000941         1779 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_87675_length_1779_cov_2.775522, whole genome shotgun sequence.
ACCESSION   DAOQUC010000941 DAOQUC010000000
VERSION     DAOQUC010000941.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1779
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87675_length_1779_cov_2.775522"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..517)
                     /locus_tag="PKH07_14285"
     CDS             complement(<1..517)
                     /locus_tag="PKH07_14285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007325677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HNT36150.1"
                     /translation="MRILVHYVVCLVVLGSSVSAFAASNRILPGTTVPGIDVLVERQF
                     DVLRGKKVGLITNHTGLSRDGMSDIDILHGASECTLVALFSPEHGIRGTADEHVSSGV
                     DEKTGLPVHSLYGQTKKPTAEMLQGLDVLVFDIQDIGTRFYTYIGTMASAMQAAKEHG
                     LDFVVLDRPNTI"
     gene            complement(528..1265)
                     /locus_tag="PKH07_14290"
     CDS             complement(528..1265)
                     /locus_tag="PKH07_14290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36151.1"
                     /translation="MQFCLRGISLALAGVLSCLLAMPALASEEGDADVLKHPGISICL
                     QSPKDRDILQLDLNLTQEELKVISDLVVSETGECALWLSACRAVLEDVSKDYQQKRDA
                     ITTIPVDEKSAAFLKTANANLDQVLSEGQKALLGIWIEEEWKMLTGAPFAPKAGASSF
                     VEFLKTRMTIPENVARRIRQDWSYYKTIELTHRQKLRKFPDLDPQNDISYLRMMRVNV
                     DEKTVQALDDQNRVTYKRIMRSQSLER"
     gene            complement(1271..>1779)
                     /locus_tag="PKH07_14295"
     CDS             complement(1271..>1779)
                     /locus_tag="PKH07_14295"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HNT36152.1"
                     /translation="QKKFGTLKPSSEIKMHLEVYRQRSDVNAIVHAHPPTATGFAVAG
                     IELSECVLPEVVVTIGGAPLVPYGTPSTDELAKAFVPYLNKADAFLLANHGALTLGTD
                     LMHAYYRMESLEHFAKILMVARQLGKVNFLTEQQVCELNELRQRFNVPGQQYPCTTCQ
                     VNPVPERK"
BASE COUNT          429 a          505 c          421 g          424 t
ORIGIN      
        1 ctatcgtgtt ggggcgatcc aaaacaacga aatcaagacc atgttctttg gctgcctgca
       61 tcgccgaagc catagtaccg atatacgtat agaaacgggt gccaatgtcc tggatgtcaa
      121 agacaagaac atccagaccc tgaagcatct cagccgtggg tttcttcgtc tgtccataga
      181 gactatgcac cggcagtccg gtcttctcat ccacaccgga actgacatgc tcgtctgccg
      241 tgccgcggat accatgctcc ggactgaata gcgccaccag cgtgcattcg gatgccccgt
      301 gaagaatgtc aatatcactc atgccatcac gtgaaagtcc cgtatggttg gtgataagtc
      361 ccaccttctt gccacgcaga acatcaaact gcctctcaac cagcacatca atcccaggaa
      421 cagtggtgcc gggaagtatg cgattcgatg cagcaaatgc actcacggaa ctcccaagaa
      481 cgacgagaca gacaacatag tgaaccagga ttctcatggc ctcgtgccta cctttccagt
      541 gactggcttc gcatgatgcg cttgtaagtc accctgtttt gatcatcaag agcctgaacc
      601 gtcttctcat ccacattcac acgcatcatt cggagatagc ttatgtcgtt ttgaggatcc
      661 aagtcgggaa actttctcag tttctgcctg tgtgtcagtt ctatcgtctt gtaataagac
      721 cagtcctgcc tgatgcgtcg cgccacattc tcaggaattg tcatccgcgt cttcaagaac
      781 tccacaaaag aactcgcacc ggctttgggt gcaaagggcg cgccggtgag catcttccat
      841 tcttcctcaa tccatatccc cagaagggct ttctggcctt ccgacagaac ttgatccaga
      901 ttcgcattgg ccgttttgag aaacgccgca ctcttctcgt ccaccgggat ggttgtgatc
      961 gcgtctcgtt tctgttgata gtccttcgac acatcctcaa gaacggcacg acaggcagat
     1021 agccataagg cgcactcccc tgtttcagac acaaccaagt ccgagataac cttgagctct
     1081 tcctgagtca gattcaagtc cagttggaga atgtcgcggt ccttgggact ttggagacag
     1141 atggaaatcc ctggatgttt taggacatcc gcatctcctt cttctgatgc caaggctggc
     1201 attgccaaaa gacaagaaag aactccagcc aaagcaagtg aaatccctct gagacaaaac
     1261 tgcattcgtg tcacttcctc tcgggaacag gattcacctg gcacgttgtg caggggtact
     1321 gctgtcccgg tacattaaat cgttgacgga gttcgttgag ttcgcagacc tgctgctccg
     1381 tcaagaagtt cacctttccg agttgtctgg ccaccatgag aatcttggcg aagtgttcca
     1441 gcgattccat tcgatagtag gcatgcatca agtccgtacc cagtgtcaac gctccatggt
     1501 tggcaagcag gaacgcgtct gccttgttca gatagggaac aaacgctttc gccagttcat
     1561 ctgtgctcgg cgtcccatag ggcacaagcg gcgccccacc gatagtgaca accacttcgg
     1621 gcagaacgca ctcagacagt tcaatacccg ccacggcaaa cccggttgcg gtgggaggat
     1681 gcgcgtgaac aatggcattt acatccgacc tctgacggta cacctcaaga tgcatcttga
     1741 tctcggatga cggcttcaga gtgccaaact tcttctgcc
//
LOCUS       DAOQUC010000942         1775 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_88007_length_1775_cov_2.718605, whole genome shotgun sequence.
ACCESSION   DAOQUC010000942 DAOQUC010000000
VERSION     DAOQUC010000942.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1775
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88007_length_1775_cov_2.718605"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1725)
                     /locus_tag="PKH07_14300"
     CDS             complement(<1..1725)
                     /locus_tag="PKH07_14300"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNT36153.1"
                     /translation="MRRSNEALAAANEELEQAIEQARLLAVEAQAASRAKGSFLANMS
                     HEIRTPMNGILGMTGLLLDTELTDEQRLFAQTVLNSAKSLLEIINDILDFSKIEAGKM
                     DLESMEFNLRTTLEDLSDILAFRAQEKGLEFACLIDPEVPLMVQGDPGRLRQVLTNLI
                     GNAIKFTQEGEILVHVSKVEEIDQSVRLRFEVKDTGIGLESTQASKLFEPFTQLDMSI
                     TRKYGGTGLGLSISKLLVEMMGGEIGVEAVPNQGSTFWFTAVLQRSAGTITETAPVLE
                     DIRGKRILVVDDNTTNRLVLRMQLHSWECRLDEASTAQEALEKLHAAQQQGDPFLIAI
                     LDMQMPEMDGEWLGKTIKQDPSISDTILILLTSIGERGEARRVENLGFAAYLTKPLHQ
                     SDLHRCLRTLAARKDIVEDDRPSRILTRHSLSESRCKKFRILVAEDNPTNQLVAVRVL
                     EKLGYRAEAVANGKEVIQALQSSVSYDAILMDVQMPEMDGYEAAGVVRNPNSPVPRHD
                     IPIIAMTAHAMVGDREKCLKAGMDDYVSKPIDVHEMAEVLARFLKVGTVEEVGTQTVL
                     RQEEKETHP"
BASE COUNT          380 a          508 c          446 g          441 t
ORIGIN      
        1 cggatgagtc tccttttcct cttgacgaag gacagtctgt gttccgactt cttccacggt
       61 acccactttg agaaaccgtg ccagaacctc agccatttca tgaacgtcta tcggctttga
      121 gacatagtca tccatgcctg ccttcaggca tttctcgcgg tcaccaacca tggcatgagc
      181 cgtcatggca ataatcggaa tgtcgtgtct gggcacaggt gagttgggat tgcgaaccac
      241 tcccgctgct tcgtaaccat ccatctcggg catctggaca tccatgagga tcgcatcgta
      301 tgaaacactg ctttgaagcg cttggatgac ttcctttcca ttggccacgg cttccgccct
      361 gtagccgagt ttctccagca cacgaacggc aaccaactgg ttggtgggat tgtcttctgc
      421 gaccaggatt cggaatttct tgcaccgcga ttctgaaaga ctgtgtcgag tgagaattcg
      481 gctgggtcta tcgtcctcga caatgtcctt tcgagccgcc agtgttctca gacatcggtg
      541 cagatcagac tgatgaagtg gtttggtcag atatgcggca aatccaagat tctccactcg
      601 cctcgcctcg cctcgctctc caatggaagt caggagaata agaatggtgt cggaaatgga
      661 aggatcctgc ttgatggtct ttcccaacca ctccccatcc atttcgggca tctgcatgtc
      721 gagaatagcg atcagaaagg gatctccctg ttgctgagcg gcatgcagtt tttccagtgc
      781 ttcctgcgcc gttgaagctt catccaagcg gcattcccag gagtgcagct gcatcctcaa
      841 aacaaggcga ttggtggtgt tgtcatccac aacgagaatc cgcttgccgc ggatgtcctc
      901 cagaacaggc gctgtttcgg tgatagtgcc agccgatctc tgcaggacgg cggtgaacca
      961 gaacgtgctt ccctggttcg gaaccgcctc gactccgatc tcgccaccca tcatctccac
     1021 cagcaactta ctgatgctca gtcccagtcc tgtgccaccg tattttcggg tgatggacat
     1081 atccaactgt gtgaacggct cgaaaagttt ggaggcctga gtagactcca aacctatgcc
     1141 tgtgtccttg acctcaaagc ggagccgaac ggactgatcg atctcctcga ctttggagac
     1201 atgtaccagt atttcccctt cctgcgtgaa tttgatggca ttcccaatga gatttgtgag
     1261 cacctgacgg aggcgtccag gatcgccttg aaccatcagg ggaacttcgg gatcaataag
     1321 gcacgcgaac tccaacccct tctcctgagc gcggaaagca agaatgtcgc tcagatcttc
     1381 aagtgtcgtt cgcagattga attccatgga ttccagatcc atcttccccg cttcaatctt
     1441 ggagaagtcc agaatgtcat tgatgatctc caacagggac ttggcactgt tgagaaccgt
     1501 ttgggcaaag agacgctgct catctgtcag ttccgtatcc agcagcaggc ccgtcatgcc
     1561 cagaatcccg ttcatcggag ttcgaatctc atgactcatg tttgccagga aagaaccctt
     1621 ggctcgactg gcggcctggg cttcgacggc caagagccgt gcctgttcga tggcctgttc
     1681 cagttcctca tttgccgctg ccagtgcctc gttactgcgg cgcaactctt ctgcgcggct
     1741 ctctgcttga ccccggaagc gactcaccag ataga
//
LOCUS       DAOQUC010000943         1775 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_88014_length_1775_cov_2.616279, whole genome shotgun sequence.
ACCESSION   DAOQUC010000943 DAOQUC010000000
VERSION     DAOQUC010000943.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1775
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88014_length_1775_cov_2.616279"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..426
                     /locus_tag="PKH07_14305"
     CDS             <1..426
                     /locus_tag="PKH07_14305"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNT36154.1"
                     /translation="PLHASQIEQCMRAGKHVLCTKPLINDAAQASSLLRAANETGKRL
                     QAETYARLGYLYYNHVYDKGAMDALVKAEEIDSNCGLIYYVWGRIRRDEGNLEEAASH
                     FQKAWSLDPKLSAAQDYLMDTQKEISRQKEEQARWLASE"
     gene            485..832
                     /locus_tag="PKH07_14310"
     CDS             485..832
                     /locus_tag="PKH07_14310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36155.1"
                     /translation="MSRIKCLLIFPLLAGMLSALVGCGFEMPKDKPVILKAIADNKGD
                     GLAITNDTSFDWNNVTFTINDRYFCTRPIVPSRKTVILNYEEFKDKTGTVFPLDQIPS
                     KYFIESDEGTGPA"
     gene            880..>1775
                     /locus_tag="PKH07_14315"
     CDS             880..>1775
                     /locus_tag="PKH07_14315"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HNT36156.1"
                     /translation="MASAKTCVVGATGAVGQEFLRILEQRDFPMSRLRLLASERSAGK
                     RIAFRGKDYTVELLTENSFEKGEIILSSAGGGTSKWFVPHAVKSGAVVVDNTSAFRME
                     PEVPLVVPEVNPHAIQRHKGIIANPNCSTIQMVVALKPLHDVSRIKRIVVSTYQSVSG
                     KGAKAMEELRLQTKALLDEKPYPPKEFPWQIAFNCLPHIDSFTDNGFTKEELKMVYET
                     QKIMEDDSIAVNATTIRVPVLRSHSESVYIETEKKITAQQAREILSKAPGVIVVDDPA
                     NNKYPMPVDSEGRDETFVGRIR"
BASE COUNT          457 a          463 c          478 g          377 t
ORIGIN      
        1 cccctgcacg catcgcagat tgagcaatgc atgcgagcag gaaaacacgt tctttgcacc
       61 aaaccactca tcaacgatgc ggcccaagcc tcctcactac ttcgtgctgc caatgaaacc
      121 ggcaagagac ttcaggcgga gacctatgcc cggctcggtt atctctacta caaccacgtc
      181 tatgacaaag gtgcgatgga cgctcttgtg aaggccgaag agatcgattc caactgcggg
      241 ctgatctact acgtctgggg tcgcatccgt cgggatgaag ggaatctgga agaggcggcc
      301 agccactttc agaaagcgtg gagtcttgac ccaaaactga gtgctgcgca ggactatctg
      361 atggataccc aaaaggaaat ctcccgacaa aaggaagaac aagctcggtg gcttgcttcc
      421 gagtagtcca ctgccagcga actgaaccat gaacggcgca tccagcgaac agagaggcct
      481 cgcgatgagc agaattaagt gtctgttgat cttcccgctt ctggcaggta tgttatccgc
      541 tctggttggg tgtggctttg agatgcccaa ggacaagccg gtgattctca aggccatcgc
      601 ggacaacaag ggtgacggtc tggcgatcac caacgacacc agcttcgact ggaacaatgt
      661 cacgttcacg atcaacgacc gatacttctg tacgcgacca atcgttccgt ctcgaaagac
      721 ggtgattctg aactacgagg aattcaagga caagacggga accgttttcc cgcttgacca
      781 gattccttca aaatacttca tcgaatctga tgaaggcaca ggtcccgcat agatccgatg
      841 actccggcta tcgggacaac aatttgaggt gtagggaaca tggcatcagc aaagacatgt
      901 gtcgtcggcg caacaggcgc tgttggacag gagtttcttc gcattctgga gcagcgtgac
      961 tttccgatgt cacgactgcg cttgctggca tcggaacgct ccgctggtaa gaggatcgcc
     1021 tttcgcggca aggattacac ggttgaactt ctgaccgaaa acagtttcga aaagggagag
     1081 atcattctct cctcggcagg tggtggaact tccaagtggt ttgttcctca tgctgtgaaa
     1141 tcgggagctg ttgttgtaga caacacatcc gcgtttcgaa tggaaccgga agttcctctg
     1201 gtcgttcctg aagtcaatcc gcacgcgatc cagagacaca aggggatcat cgcaaatccg
     1261 aactgctcga cgattcaaat ggttgtggcg ctcaagccac tccatgatgt ttcccgcatc
     1321 aagagaattg tcgtctccac ttatcagtct gtttccggca agggtgcgaa ggccatggag
     1381 gagctgaggc ttcagacaaa ggcgttgctg gatgaaaagc cctatccacc gaaggaattc
     1441 ccctggcaga ttgccttcaa ctgtctgcca cacattgata gtttcacgga caatgggttc
     1501 accaaagaag aattgaagat ggtgtacgaa acccagaaga tcatggaaga cgacagcatc
     1561 gccgtcaacg cgacaacgat tcgcgtgccg gtgctccgaa gccacagcga gagcgtctac
     1621 attgagaccg agaagaagat cacggcgcag caagcccgcg agattctttc aaaggctcct
     1681 ggcgtgatcg tcgtggatga tcctgccaac aacaagtatc cgatgcccgt tgactcagag
     1741 ggacgggatg aaaccttcgt tggtcgcatt cgaga
//
LOCUS       DAOQUC010000944         1774 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_88106_length_1774_cov_2.472949, whole genome shotgun sequence.
ACCESSION   DAOQUC010000944 DAOQUC010000000
VERSION     DAOQUC010000944.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1774)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1774)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1774
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88106_length_1774_cov_2.472949"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            332..574
                     /locus_tag="PKH07_14320"
     CDS             332..574
                     /locus_tag="PKH07_14320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36157.1"
                     /translation="MSRKGGWFPPFHTATVVRAKTQDGDAEIPWHYWQNSVLDDWQPL
                     TALHGFGFTDLYEMWQFLLKYPFYDEYKDDQCVSPR"
     gene            556..1041
                     /locus_tag="PKH07_14325"
     CDS             556..1041
                     /locus_tag="PKH07_14325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36158.1"
                     /translation="MRVSTLTSIVIGVPFLLVILVGIALLIIICINSWESRQTYHDAT
                     LMHKPGMESFFSNRKMDEFLPADIPYETIYWFDAETDRRFSGLTPVTRLFGLYGVRIN
                     PKYGDLKVNGVEVPGAIGFIACPGIDQQANYYEEHNSVIEFRTSNGRRSGGDIIFIFH
                     Q"
     gene            1122..1592
                     /locus_tag="PKH07_14330"
     CDS             1122..1592
                     /locus_tag="PKH07_14330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36159.1"
                     /translation="MRLTNRRLIGLGILLIFLFLMLHLYAHLYNEGRIGAPRDSANRF
                     LWGYSFIRTGVKRSMVDRVLPQSLYERVSFRRATGDDIMVFFPAGISAELRDTVASMP
                     RAIESFDELTPVKSGLIISFKGDTVDGLEWKLYKEGVLREQTEFKKLKVPWLNE"
BASE COUNT          451 a          385 c          485 g          453 t
ORIGIN      
        1 cttccacgcc cgctggtacg acccggaagt ggggaggttt gtgagcaggg atccggtcgt
       61 ttctcacagc gctacgcagt acagtcttgc agagaatagc ccaaccactt tgatagatcc
      121 ttcgggtgca attcctatga caccttggaa ggctaagcct gcatgttcga gggcggaaag
      181 aaacaaggag tggtttggta cgataatacc gaaattcagg gaggcgaatc aggaaaacaa
      241 cattaatcgc acaaaggact gtgtaagcag agcctgtaag ataacagaca ggatttcgag
      301 agctgggatt tgctgtcatg aactgcgtga gatgagccgg aaaggaggtt ggtttcctcc
      361 ttttcacacc gcaactgttg tcagagcgaa aactcaggat ggcgatgccg aaataccatg
      421 gcactactgg caaaactctg tgctcgacga ttggcagcct ttaacggctc ttcatggttt
      481 cggattcacg gatctgtacg aaatgtggca attcctgctc aagtatccat tttacgatga
      541 gtataaggat gaccaatgcg tgtctccacg ttaacatcaa tcgttatcgg agtgcctttt
      601 ctccttgtga tcctggtcgg gattgcattg ctcatcatta tctgtatcaa cagttgggag
      661 tcacgacaga cgtatcatga tgcgacgctc atgcacaagc caggaatgga atctttcttt
      721 tcgaacagaa agatggatga attcttacca gcggatatcc cttacgaaac gatatattgg
      781 tttgatgctg agactgacag gcgtttcagt gggttgacgc ctgtgacgcg acttttcggt
      841 ctctatggcg tacggatcaa cccgaagtat ggggatttga aggtgaacgg cgttgaagta
      901 cccggcgcaa ttggattcat agcctgcccc ggaattgatc aacaagcaaa ttactatgag
      961 gagcacaata gcgtcataga gttccgtacc tcgaacggca ggagaagcgg cggcgatatc
     1021 atcttcatct ttcatcaata gttcccttag tttgttggag tttggatagg gtctctttga
     1081 gtgggagctg cttggagtca aacgcttctg agaggggttt catgagactg acaaatcgaa
     1141 gactgatcgg attgggtatt ctattgatat tcctctttct gatgctccac ctttacgctc
     1201 atctctacaa tgagggcagg ataggagctc ctcgtgattc agcgaaccgg tttctttggg
     1261 ggtactcgtt catacggacg ggggtgaaga gaagtatggt tgatcgcgta cttccccagt
     1321 cgttatatga gcgcgtttct ttccgcagag caacagggga cgacattatg gtgttcttcc
     1381 ctgcgggaat ctctgccgag cttcgcgata ctgttgcatc aatgccgcga gcgatagagt
     1441 ctttcgatga actgacacca gttaagagtg gactaataat ctcgttcaag ggggatacgg
     1501 ttgacggctt ggagtggaaa ctctacaagg aaggtgtttt gcgagaacag acggagttca
     1561 agaagttgaa agtcccttgg ttgaatgagt agctgctgtg ttgcgtaatg tgcgtgtagc
     1621 gaatagtccg tagcgggagc ctggactcca tgcgaaatgg accatacaga ccaaatatga
     1681 gagacttaac atcatgatga cttcctggcg ccaagatgaa acaggagccc tcagaaccgc
     1741 ttcgcgaacc tgagggctgg cacccagaga agtc
//
LOCUS       DAOQUC010000945         1773 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_88170_length_1773_cov_2.593714, whole genome shotgun sequence.
ACCESSION   DAOQUC010000945 DAOQUC010000000
VERSION     DAOQUC010000945.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1773
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88170_length_1773_cov_2.593714"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..442)
                     /locus_tag="PKH07_14335"
     CDS             complement(<1..442)
                     /locus_tag="PKH07_14335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HNT36160.1"
                     /translation="MPTLTIDQKTIEVPDGTTILQAARQAGIRIPTLCFLDNYPPIGA
                     CRVCLVEVEGARTLVASCSTPVAQGMKVRTNSRRVREGRRTVVELLLSEHEGDCQTCV
                     RAGDCELQSLARELGIRDVRYPGEKTRRLVDTSTPALTRDTGKCI"
     gene            complement(506..>1773)
                     /locus_tag="PKH07_14340"
     CDS             complement(506..>1773)
                     /locus_tag="PKH07_14340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014455791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNT36161.1"
                     /translation="CRRSTLDESVSIRGVKRFMVEQEVTIQYPEVREDENNAKRKVAV
                     VGAGPAGLSCAYFLARLGYKPRVFEAAPRPGGMLVQTIPAYRLPREVLAREIRMIERL
                     GADIETNSHLGKDFTLQSLKEEGYEAVFIGVGAPQGVKMGIPGEELDGVVDAIGFLRE
                     YNIRGSVPVGNKVVVVGGGNAAIDAARTAVRLGSKEVTIIYRRTRSEMPAYAEEVEEA
                     EREGVRIRLLAAPLEVVGDKGKVKGLKCTLMGLGDFDRSGRRRPVAQESSEFIVEADQ
                     VIAAIGQSLDANEIVNGTEVKITRKGFIEANPVTGQTSIPWIFAGGDSATGPWSVIEA
                     VAGGERAAVGIDQYLTGENHAFWREEQEVDTYFDPNADPVLYPRPKVRLMPVNKRRHN
                     FDEVELPWSEGVAQREAKRCLRCDYREKC"
BASE COUNT          375 a          558 c          450 g          390 t
ORIGIN      
        1 gaatgcactt gccggtgtcg cgagtcagcg ctggagtgct ggtgtcaaca agacgccggg
       61 tcttctctcc cggatagcgt acgtcgcgga tgcccaattc ccttgcgaga ctctgcaact
      121 cacagtctcc agctcgaacg caggtctggc agtccccttc gtgctctgag agaagcaact
      181 caaccacggt tcttcgacct tcacgaacgc gacgggagtt ggtgcgaacc ttcatcccct
      241 gagccaccgg agtagagcat gaggccacca gagttcgtgc cccttccact tcgaccagac
      301 aaacccgaca cgctccaatc ggagggtagt tgtcgagaaa gcacaaggtc ggaatccgaa
      361 tccccgcctg tctcgctgct tgaagaattg tcgtcccgtc ggggacttct atcgttttct
      421 gatcaatcgt tagcgttggc atctactgac tccttgacac tgttgctgca tccaatcccg
      481 actccgcgga gcggctgccg ctcgcttagc acttctcccg atagtcacag cgcagacagc
      541 gctttgcttc acgctgtgcc acaccttcac tccaaggcaa ctcgacttcg tcgaagttgt
      601 gacgccgttt gttcaccggc atcagcctca ccttggggcg gggatagagc actggatccg
      661 cgttgggatc aaagtaggta tccacttcct gctcctcgcg ccagaaagcg tgattctctc
      721 ctgtcagata ttggtctatt ccaaccgcag cccgctcacc accggccacg gcttcgatca
      781 ccgaccaagg acccgtcgcc gaatcgccac cggcgaatat ccatggaatt gaagtctgac
      841 ccgtcacggg attggcttcg atgaaaccct tgcgggtgat tttcacttcg gtgccattga
      901 cgatttcatt ggcatccaac gattggccga tcgccgcgat gacctggtct gcttcaacga
      961 tgaactcact ggactcctga gcgaccggac gacgacgacc ggaacggtcg aaatcaccca
     1021 gacccatcaa ggtgcacttg agccccttga ccttgccttt gtctccgaca acttccaacg
     1081 gagcggccaa gagtcgaatc cgaacgcctt cgcgttccgc ctcttcgact tcttccgcat
     1141 acgcaggcat ctccgaacga gtcctgcgat agatgatagt gacttccttt gaaccaagac
     1201 gcaccgcagt tcgggctgcg tcaatggctg cgttgccacc acccacgacc acaactttgt
     1261 tcccgacggg cacggaaccg cgaatgttgt actcccgcaa gaaaccgatg gcgtccacta
     1321 cgccgtccag ttcctctcca gggataccca tcttgacacc ctggggtgcg ccaacaccga
     1381 tgaagactgc ttcgtaccct tcttctttga ggctctgaag cgtgaaatcc ttcccaaggt
     1441 gcgaattggt ctcaatgtct gcacccagtc gctcaatcat tcgaatctca cgggcgagaa
     1501 cttctcttgg caaccggtag gcggggattg tctgaaccag catacctcct gggcgaggcg
     1561 ccgcttcaaa gacacgaggc ttgtagccga gtctggccaa aaagtaagca caggacagac
     1621 ccgcagggcc ggcaccgacg acagcgactt tgcgcttggc gttgttttcg tcttcacgca
     1681 cttcaggata ttgaattgtg acttcctgct cgaccataaa gcgtttgacg ccgcgaatgg
     1741 aaacagactc atcaagtgtg gaacggcggc att
//
LOCUS       DAOQUC010000946         1768 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_88542_length_1768_cov_3.991243, whole genome shotgun sequence.
ACCESSION   DAOQUC010000946 DAOQUC010000000
VERSION     DAOQUC010000946.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1768)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1768)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1768
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88542_length_1768_cov_3.991243"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(59..>1768)
                     /locus_tag="PKH07_14345"
     CDS             complement(59..>1768)
                     /locus_tag="PKH07_14345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36162.1"
                     /translation="DYGGYVATAAGQIMTFGTTAVDVSGFVPRVGRTITDLLLDREQD
                     GLYVLYDNGAIDTLGNATRFSLSPILAGARAVALRLSSSGNGLYMLDSYGGVYAVGDA
                     GSFGSEVPYFGFDVARGFIVSATNSGLAVMTGDGRVHPSRGVAVARDYNVSPSYQDIF
                     RDIEVIGGKKIAILNMLEEIYSALEREDLIALMRHISPNYFDENYNTRALYELARRNF
                     FDFYMTREWKISAMRDNPLIIIAGPKAYATVYEDWSFYIPSLTSVRLDRPVGGGGGAF
                     SLAVNFVVPFTQVFRWYLNADTEWRYYGMKLNVYDLDHEGTEFDPRFDREVRTYGALP
                     SQGSERLDYEGAGTGPKFDNRYQYFFSEGPNANGQAGPFDVNWMYYDGDIVSASGFES
                     HWEFDLENGTWAVTRSDFIQFLFAHGVSGATDTTGEGTGFGGGFDFVQRATVIDDNVK
                     KISHVRYTGGSLSSPFYPNQGGVVDLTESIPDMFTGIYPTSFDEVTVAEIRNIADVYW
                     SQSVPVVPPQEDPEFRTHIYAVKFPGTKQPFGLIQVLGSLTGDMDGIYFRWRYRDDFR
                     LSP"
BASE COUNT          458 a          500 c          439 g          371 t
ORIGIN      
        1 ctctcacaaa gagaggcttt cggcaaaacc gattcagatc ccagaaccgg gacacccact
       61 aaggtgacag tcggaaatca tcccgatagc gccaccggaa gtagatacca tccatgtccc
      121 ccgtgagcga tccaagaacc tggatcaagc caaacggctg cttggttcca gggaacttga
      181 ccgcatagat atgcgttctg aactcaggat cttcctgtgg gggcaccaca ggaacgctct
      241 ggctccagta gacgtcagca atgtttcgta tctctgcaac agtgacctca tcaaagctgg
      301 tcggatagat tccagtgaac atatccggga tactttcggt caggtcaacc actccaccct
      361 ggttcggata gaacggagag gacagactcc caccagtata acgaacgtgg gagatctttt
      421 tgacgttgtc gtctatgacc gtggctcgct ggacaaagtc aaagccacca ccaaaacctg
      481 ttccctcacc agtcgtgtcg gttgccccgg aaacaccgtg tgcaaacagg aactggataa
      541 agtcagagcg tgtaaccgcc caagttccgt tctccagatc gaactcccag tgggattcaa
      601 aaccagaagc cgaaacgatg tctccatcgt agtacatcca gttcacatca aacggtcctg
      661 cctgaccatt cgcgtttgga ccctcgctga agaagtactg gtaacggttg tcgaattttg
      721 gaccagtgcc cgctccttca tagtcaagac gctcggatcc ctgactcggc aaagctccgt
      781 aagtgcgcac ttcacggtca aaacgcgggt caaactccgt tccttcgtga tccaggtcgt
      841 acacattcag cttcatgccg tagtatctcc attcggtgtc ggcgttgaga taccaacgga
      901 agacctgggt gaacggaacc acgaaattca ccgccagaga gaaggcacct cctccaccac
      961 caacaggacg atcaagacgc accgatgtca gtgaaggaat gtagaagctc cagtcttcat
     1021 acacggtggc gtaagccttt ggtccggcga tgatgatgag aggattgtcc ctcatggcgc
     1081 taatcttcca ctccctggtc atgtagaagt caaagaagtt gcgacgcgca agttcataga
     1141 gcgcacgagt gttgtagttt tcgtcaaagt agttcggtga gatgtgtctc atcagcgcga
     1201 tcagatcttc tcgctccaac gcggagtaaa tctcttccag catgttgagg atggcaatct
     1261 tcttaccacc gatgacttca atgtcacgga agatatcctg atagcttggt gaaacattgt
     1321 agtcgcgcgc gacggcaacc cctcgggatg ggtgtacacg accgtcgcca gtcataacag
     1381 ccagtccgga gttggtggcg gatacaatga agcctcgcgc cacatcaaag ccgaagtacg
     1441 gaacttcaga gccgaacgat cctgcatcgc caaccgcgta gacgccacca tagctgtcca
     1501 acatatacag cccatttccg ctcgatgaca atcgcaaggc aacggctctg gcacccgcaa
     1561 gaataggact cagagagaag cgagtggcat tgcccaacgt atcaatcgcg ccgttgtcat
     1621 acaggacata gagaccatcc tgttcgcgat ccaacagcag atcggtaatc gttctgccaa
     1681 ctctgggcac gaaaccagaa acgtctactg cggtcgtgcc gaaagtcatg atctgccctg
     1741 cggcggtggc aacgtatcca ccatagtc
//
LOCUS       DAOQUC010000947         1764 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_88878_length_1764_cov_3.153891, whole genome shotgun sequence.
ACCESSION   DAOQUC010000947 DAOQUC010000000
VERSION     DAOQUC010000947.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1764
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88878_length_1764_cov_3.153891"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1764
                     /locus_tag="PKH07_14350"
     CDS             <1..>1764
                     /locus_tag="PKH07_14350"
                     /inference="COORDINATES: protein motif:HMM:TIGR02276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36163.1"
                     /translation="AADQARNLVYSVNRETNSVSVINGNTNAVIETFPVGRDPEDAIL
                     IPDLNRLLVALKSEHSLAVVDVLTRTVLEKKYLGSELGEDKRPHRIAYNSLTGKAYVS
                     NSWSGDVSVLSVKPFAFVENIQVDSGTKVYGIAVNEVSNEVYVSDAYGRVAVIDGSTD
                     SVSSYVDLGGKAPNALAYDSVADRMFLTCFGFPTPKEILVVTRKQLTGAIAMPEPILS
                     LLVDESSRRLYATGFIYGKLYEVDMDTLNIVSSTDVGWEGLAQALNKSTGCLYVGGHK
                     GGAYSVVRVGEKTVPEPPEIISVTGDSQALVEWTSGNPASLFNVYRSRSSAGPFVRLN
                     YAPLPAHERRFIDPTVVNGNTYFYAMSGVDEHGVEGALSAPGVAFVPQESVTPDFRLS
                     SVEVAVALQPATSATATLLLQPLNGFSSKVSLSAVSVPDGLTLSFDPQETFAPAAVVF
                     SVTASPLLLPCVLPVVIGASASGYEKDLYFFVNVRETSAGGIRVVSFDETQDAVLRQG
                     IQVMLTTDVGSHARDGLVTLKGRIQGVPSVSSCRVIVTKPDGSVENVDNVMVTNGMFG
                     LSLPVLSESEYGIWRAVVVWQG"
BASE COUNT          363 a          431 c          504 g          466 t
ORIGIN      
        1 tgctgcagac caggccagga atctcgtcta ctcggtcaat cgtgagacca acagcgtttc
       61 tgtcattaat ggcaacacca atgctgtcat tgagaccttt ccggtgggac gagatcctga
      121 agacgcgatt ctcattcccg atctgaatcg ccttctggtt gctctcaagt ccgagcacag
      181 tcttgcggtg gtggacgttt tgacgcgaac cgtactggaa aagaaatatc tgggttccga
      241 actgggagaa gacaagagac ctcatcggat tgcctacaac agtttgacag ggaaagcgta
      301 tgtttccaac tcctggagtg gagacgtttc cgttttgagc gtcaagcctt ttgcctttgt
      361 tgaaaacatt caggttgact ccgggaccaa agtctatggc attgccgtca atgaagtcag
      421 caatgaagtt tacgtttcag acgcttacgg acgtgttgct gtcattgacg gttcgaccga
      481 ttccgtttct tcctatgttg acttgggagg caaggcacca aatgctcttg cctacgactc
      541 cgtggcggat cgaatgttct taacctgttt tggattcccg actccgaagg agattcttgt
      601 tgttacccgt aagcaactga ctggtgccat cgccatgccc gagccgatat tgagtcttct
      661 tgtggacgaa tcgagtcggc gactttacgc caccggattc atctatggga aactctatga
      721 agttgacatg gatacgctga acatcgtttc atcaacagac gttgggtggg aggggcttgc
      781 gcaggctttg aacaagtcca cgggctgcct gtacgttggt gggcacaagg gaggggcata
      841 ctccgttgtg agagttggtg agaagacagt tccggagcca ccagagatca tatcggtcac
      901 aggagacagc caggctcttg tcgaatggac atccggtaat cctgcgagtc tattcaacgt
      961 ctatcgttct cgttcgagtg ctggcccctt cgtgcggctt aactacgccc ctcttcctgc
     1021 tcatgagaga agattcatcg atcccacggt cgtgaatggg aacacttatt tctatgccat
     1081 gagcggggtg gacgagcatg gggtggaagg tgccctctct gcacccggtg tggcatttgt
     1141 tcctcaggaa tcggtgacac ctgactttcg actcagttcg gttgaggttg ccgtggctct
     1201 acaacctgca acatccgcga cagcaacact actgctccag cctctgaacg gattcagttc
     1261 caaggtctct ctttcggccg tctcggttcc cgatgggctc acgctgtcat tcgatccaca
     1321 ggagacattt gcaccggccg cagtcgtgtt ctcggttacg gccagtccgc tgctgctacc
     1381 ttgcgttttg cctgtcgtga taggtgcatc tgcttcggga tatgagaagg atctctattt
     1441 ctttgtgaat gtccgggaga cttctgctgg tgggattcgg gtggtgtctt ttgacgaaac
     1501 acaggatgca gtgcttcgtc aagggataca agtgatgctg acaaccgatg tggggagcca
     1561 tgctcgggac ggtctggtca ctttgaaggg tcggatacag ggtgttccga gtgtttcatc
     1621 ctgccgggtg atcgtcacta agccggacgg ttcggtcgag aatgtggaca acgtgatggt
     1681 gaccaacggt atgtttggtt tgtcactgcc tgtgctgagc gagtctgagt acggaatctg
     1741 gcgggccgtt gtcgtgtggc aagg
//
LOCUS       DAOQUC010000948         1764 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_88890_length_1764_cov_2.905208, whole genome shotgun sequence.
ACCESSION   DAOQUC010000948 DAOQUC010000000
VERSION     DAOQUC010000948.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1764
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88890_length_1764_cov_2.905208"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="PKH07_14355"
     CDS             <1..230
                     /locus_tag="PKH07_14355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36164.1"
                     /translation="NVFFKKEKFREAAREFQACLDVDRKNIQAHKNLALTWYRIGDLR
                     NSASEWQSALNLDQTDKQAQENLATLTKHIS"
     gene            239..865
                     /locus_tag="PKH07_14360"
     CDS             239..865
                     /locus_tag="PKH07_14360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36165.1"
                     /translation="MQMHSVEVYIPWLQTVLGVLLLYCGWLLYRVGMMCLSWCLGAIG
                     GGCLAKVLIQLVREIPNVWWVVVIGAIVGGIVGVFLIRRLYFVALFVISGLFALNLKQ
                     TVLTNPRLFSALNLSQLERFWNSASGTVVFAVAFALLVVALHRFLVIWLTSGVGALLV
                     ADRLPWQQAVYFLAVTGVIVQLGLIRGFGIDTGRETNRWKQERKAEDS"
     gene            832..1461
                     /locus_tag="PKH07_14365"
     CDS             832..1461
                     /locus_tag="PKH07_14365"
                     /inference="COORDINATES: protein motif:HMM:NF019934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspG"
                     /protein_id="HNT36166.1"
                     /translation="METRTESRRQLVAAFIACLVLCAVFSGCGGAGQDSASSEQGFSG
                     TDDSNAPKGVVVKDDAIKLPEDPQARAVIGVRYIAFGMLLFKSHVGRLPTEEEGISAM
                     YEEPEAIKGQQLWRGPYNDAEIVKLDPWGHDYQYRVDPELGLGYEVWSLGADGVKSDD
                     DIIASRDAGLPGFLGQGYPMQVEEDHVHAPRAPENPVPVPGDANLPHQH"
     gene            1476..>1764
                     /locus_tag="PKH07_14370"
     CDS             1476..>1764
                     /locus_tag="PKH07_14370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36167.1"
                     /translation="MIRKPIGKVISTQWDEQAKRLDFVVEFADNGYRISGSLKSGFDP
                     ASGSWVSAEALKKKAQEMLSMKVYDDGSGLGEGALTPERLTNLRLKVSLSSA"
BASE COUNT          403 a          414 c          533 g          414 t
ORIGIN      
        1 gcaatgtgtt tttcaagaag gagaaatttc gtgaagccgc aagggagttt caggcctgtc
       61 ttgacgtaga cagaaagaac attcaggctc acaagaatct ggcgctaacc tggtaccgaa
      121 ttggggactt gaggaactcc gcttcagagt ggcagagcgc cctgaatctt gaccagaccg
      181 ataaacaagc acaggagaat cttgccacgt tgacgaagca tataagttga gggtcgccat
      241 gcagatgcac tccgtcgaag tctatattcc gtggctacaa accgtactgg gcgttcttct
      301 tctgtactgc ggatggctcc tgtatcgcgt ggggatgatg tgcttgtcct ggtgcctggg
      361 tgctatcggt ggtggctgtc ttgcaaaggt gctgatccaa ctggtgcgtg agattccgaa
      421 tgtctggtgg gtggttgtaa ttggggctat tgtcggcggc attgttggag tgttcctgat
      481 tcggcgtttg tactttgtcg cccttttcgt catctcagga ctctttgctc tcaatctgaa
      541 gcaaacggtt ctcacaaacc cacgtctttt cagtgccctg aatctatcgc aactggaaag
      601 attctggaat agtgcatcgg gaacggttgt tttcgctgtg gcttttgcac tgctggtagt
      661 tgcgctccac cggtttcttg taatatggtt gaccagtggc gtcggagcct tgctggtcgc
      721 cgacaggctt ccctggcagc aggcagtgta tttcctggcg gtgaccggcg tgattgtcca
      781 gctgggcttg atacgaggat tcggaataga cacggggaga gagacaaacc gatggaaaca
      841 agaacggaaa gcagaagaca gttagtggcg gctttcatcg cctgcctggt tttgtgtgca
      901 gttttttccg gctgtggagg cgcaggccag gattctgcct catccgaaca gggattcagt
      961 gggacagatg attccaacgc tccgaaaggc gtcgtcgtaa aggatgacgc aatcaagctt
     1021 cctgaagatc cccaggcacg tgcggtgatc ggagtcaggt acattgcctt tggaatgctt
     1081 cttttcaaga gccacgttgg caggctcccg acagaggagg aaggcatctc ggcgatgtac
     1141 gaagagccgg aagccatcaa gggacagcag ttgtggcgag gcccctacaa cgacgcggaa
     1201 atcgtcaaac tggatccatg gggacatgac taccagtatc gagtggatcc cgagctgggc
     1261 ttgggatacg aggtttggag ccttggggca gatggggtca agagcgacga cgacatcatt
     1321 gcaagtcggg atgcgggact ccctggattt ctgggtcagg gatacccgat gcaggtcgag
     1381 gaggatcatg tgcatgcccc acgagcaccc gagaacccgg ttcccgtgcc gggagacgcc
     1441 aatctccctc atcagcactg agaacttcaa caaccatgat tcgaaaacct atcggcaaag
     1501 tgatctcgac acaatgggat gagcaggcga aacgactcga ttttgtggtc gagtttgccg
     1561 acaacgggta tcggatatcc ggctcactca agagtggatt tgatccggca agcgggagtt
     1621 gggtctctgc ggaagcattg aagaagaaag cacaggagat gctttcgatg aaagtctacg
     1681 acgatggaag tggactcggc gagggcgctt tgactcccga acgtcttaca aacctacggc
     1741 taaaggtgtc tctctcaagc gctg
//
LOCUS       DAOQUC010000949         1763 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_88958_length_1763_cov_3.123536, whole genome shotgun sequence.
ACCESSION   DAOQUC010000949 DAOQUC010000000
VERSION     DAOQUC010000949.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1763
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88958_length_1763_cov_3.123536"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..656
                     /locus_tag="PKH07_14375"
     CDS             <1..656
                     /locus_tag="PKH07_14375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014958290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNT36168.1"
                     /translation="KQIDESSTETAKILKTIDEIAFQTNLLALNAAVEAARAGEAGKG
                     FAVVAEEVRNLAQRSAQAAKNTAELIENAQKHAAAGVSVSAEVAGHLNGINEGTQKIL
                     TLVSEIATASTEQAQGIAQVNTAVSEMDKVTQDNAANAEETASAAQEMSAEAQILNGL
                     VDDLNAVITGSKGQTDQGIASPESSKTRFSAAPVPQRRIGGSRLLIGKKDTDQAEDH"
     gene            complement(803..>1763)
                     /locus_tag="PKH07_14380"
     CDS             complement(803..>1763)
                     /locus_tag="PKH07_14380"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNT36169.1"
                     /translation="GAETAEEVNAKVLPWLKSNATRENYYLHINYWDAHRCYKMDASW
                     ADRFRDYPVTQSWPDEEAIRTHQSVTGAFTAHRQFQNDTSPHPLMPGAINDRRDFEHM
                     ITGYDASIAYVDAHVKQVLDELDRQGVLEDAVIVFSADHGDAFGEHGIYSDHVNVDEC
                     IHRIPLIIRWPGVTPAHVRSNTFMYNVDYAPTICDLLGMPIPEDWDGKSYQDNLRGLS
                     GADREWLVWDGALYTVQRAVRTKTHLYIKTVDAWEYNNRSDEELYDMIADPYETTNLC
                     DSSPDVLEQCRTIMCDWIDQQKCKANWTADPFDAILEHRKSWR"
BASE COUNT          404 a          460 c          502 g          397 t
ORIGIN      
        1 ttaaacagat cgatgagtct tctaccgaga ctgccaagat tctgaagacc attgatgaga
       61 ttgctttcca gaccaacctg cttgctctga acgcggcggt ggaagcggct cgagccggtg
      121 aggcgggcaa aggctttgcg gttgtggctg aggaagtccg caatctggcg cagagaagcg
      181 ctcaggcagc caagaatacg gccgagttga ttgagaatgc gcagaagcat gccgccgctg
      241 gggtctccgt ttcagcagag gttgcaggcc atctgaatgg tatcaacgaa gggacacaga
      301 aaattctgac gctcgtcagt gagattgcca cagcgtctac agaacaggcg cagggaattg
      361 ctcaggtcaa caccgccgtg tcggaaatgg acaaggtcac tcaggacaac gctgccaacg
      421 ccgaagagac agccagcgcc gctcaggaga tgtctgcgga ggctcagatt ctcaacgggt
      481 tggtagatga tctgaatgcc gtgatcactg gttccaaggg acagacagac caaggaatcg
      541 caagtcccga aagcagtaag acacgttttt ccgccgcgcc agtcccccaa agacgcatcg
      601 gtggctcgcg acttctgatc gggaagaaag acaccgacca ggctgaagat cactgagggg
      661 acagacacat gaccccctat tgctcacagc agaatgcaat aggtgaagtc tgaaagaaca
      721 agcgctccct ttctccattg tgggaaaggg agcgcttttt ttttgagtta ccgctcttac
      781 agcaactact gcgtgacaga gtctatcgcc agcttttcct gtgttccaga atcgcgtcaa
      841 acggatcggc cgtccagttg gccttgcatt tttgttgatc gatccagtcg cacatgattg
      901 ttctgcattg ctcgagaaca tccggggaag agtcgcacag gttggttgtt tcgtaggggt
      961 cggcaatcat gtcgtacagc tcttcatcag agcggttgtt gtattcccag gcatccacgg
     1021 tcttgatgta cagatgcgtt ttcgttcgaa cggcacgctg caccgtgtaa agagccccgt
     1081 cccagacgag ccattcccga tctgcgccgc tcaatcctct gaggttgtcc tggtacgatt
     1141 tgccatccca gtcttccgga atcggcattc ccagcagatc acagatcgtt ggagcgtagt
     1201 ccacgttata catgaaggta ttcgatcgca cgtgggcagg agtgacgcca ggccaacgga
     1261 tgatgagtgg aattcgatgg atacactcgt ccacgttgac gtgatcggag tagattccgt
     1321 gctcgccaaa ggcgtcccca tgatcggctg agaagacgat caccgcatcc tccagaactc
     1381 cctgccgatc cagctcatcg aggacttgct tgacgtgcgc gtccacatac gcgatggatg
     1441 cgtcgtaacc ggtgatcatg tgttcaaagt cgcgccggtc gttgatcgct ccgggcatca
     1501 atggatgcgg gcttgtgtca ttttggaatt gccgatgtgc tgtgaacgct ccggtgacac
     1561 tctggtgagt gcggatggct tcttcatcag gccaggactg tgtgacagga tagtctctga
     1621 aacgatccgc ccaggatgca tccatcttgt aacaccgatg agcatcccag tagttgatat
     1681 gaagatagta gttctcgcgg gtggcattgc tcttgagcca gggaagaacc ttggcgttga
     1741 cttcctcggc agtttcggcc ccg
//
LOCUS       DAOQUC010000950         1761 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_89134_length_1761_cov_2.492966, whole genome shotgun sequence.
ACCESSION   DAOQUC010000950 DAOQUC010000000
VERSION     DAOQUC010000950.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1761)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1761)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1761
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89134_length_1761_cov_2.492966"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1746)
                     /locus_tag="PKH07_14385"
     CDS             complement(<1..1746)
                     /locus_tag="PKH07_14385"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNT36170.1"
                     /translation="MVIHLEPGGSISGHVFQSFGRSPVPETRVIARPGGRGRGRGGFW
                     GDSPEAVEAITDEAGHYVLPFLALNQQYVLLAKAQEFAPASIEDVTPNSQGIDFYLNP
                     GGAISGTVTLASTGDPVQNARVRTIQRSREFGPNMTDAIQTARTDESGKYRLQGLGPG
                     RYSLFADKEELVSTSSIDFPEIILEMAEEKTGVNLTLEPGTSISGRVVDPEDRPVEGA
                     LVAISPPFGGFADTPMATTLSDDEGRFEFRRLMTGVTRYGLWAAKENLRSDNVEINMT
                     PGAQYQEVKLVLTSGFTIRGKAYVKDTEIAVAEVQLLLALLQSRGDSKSDYSDGEGRF
                     EFAGVPSGQYTLRVTPPQGLLAIEPMTTLTVADTDLLNVTVELRLGRTQMGLVLDPDG
                     HPVPDAQVYFAADRSARGMEYSDVALRSTTDAEGRFEIRNLPQEGTFRLIGRSALFAP
                     TVSQPIELITGATPDLKLTLQRGGTIAGLITDASKTPVREGVISFEFRQSEEDGLALP
                     STAMNFLNRSTARVDSSGKYSSEVLRVGQWSVQPRGVGGEVRAKTAEVREDTVTSVDF
                     TVPRAVSLSGSFSGTV"
BASE COUNT          384 a          510 c          483 g          384 t
ORIGIN      
        1 gactgttcca gagaaacttc cgctgagtga gacggcacga ggtactgtga aatcaaccga
       61 agtaaccgtg tcttcgcgga cttccgcagt cttggcgcga acctccccac cgactccgcg
      121 aggctgcact gaccactgtc cgactctgag gacttccgag gagtactttc ctgaggaatc
      181 cacccgagcc gtgctacggt tcaagaaatt catcgcggtg gagggaagag ccaaaccgtc
      241 ttcctcactt tgtcggaact caaaggagat aacaccctcg cgaacaggtg tctttgaagc
      301 atccgtgatc agaccggcaa tggtgcctcc cctctggagc gtcaacttca aatcaggagt
      361 ggcacctgtg atcagttcga ttggttggct cacagttggg gcaaatagag ccgaacgtcc
      421 gatgagtctg aatgttccct cttgcgggag atttcggatc tcaaaccgtc cctctgcatc
      481 tgtcgtggag cgcaatgcga catccgagta ttccattccg cgcgccgagc gatctgcagc
      541 aaaatacacc tgcgcatccg gaacaggatg accgtccggg tcaagaacaa gacccatctg
      601 tgtccgtccg agacgcagtt cgacggtgac attgagcaga tcggtgtccg ccacggtcaa
      661 agtggtcatt ggttcgatgg cgagaagtcc ttgtggtggt gtcacccgaa gggtgtattg
      721 gccggaagga actcccgcaa actcaaatcg tccttcaccg tccgagtagt cagacttgga
      781 atcacctctg ctctggagca atgccagcag gagttgaacc tccgcaacgg cgatctcagt
      841 gtctttgaca taggcctttc ctcggatcgt gaatcccgaa gtcagaacca gtttcacttc
      901 ctgatactga gctccaggag tcatattgat ctcgacgttg tcagatctca ggttctcctt
      961 ggccgcccaa agcccgtagc gcgtcactcc cgtcatcagg cgtctgaact caaaacggcc
     1021 ttcatcatca gaaagggtgg tcgccatggg tgtatccgca aatcccccaa agggaggaga
     1081 aatcgcgacc agagcgcctt cgacaggcct gtcttccgga tccacgacac gtccggagat
     1141 cgaagttccc ggctccaacg taagattgac accggttttc tcctcggcca tctccaggat
     1201 gatctcgggg aaatcaatgc tggacgttga aacaagctct tccttgtcgg caaacaggga
     1261 gtatcggccg gggccgagtc cctgcaatcg gtactttccg ctctcatcgg tgcgcgcggt
     1321 ctgaatcgca tcggtcatgt tggggccgaa ttcccgactg cgttgaatgg ttcgcactcg
     1381 ggcgttctgc accggatctc cggttgaagc gagtgtgacc gttccagaga ttgccccgcc
     1441 tggattcaga tagaaatcta tgccttgaga gttcggggtg acatcttcga tggaagcagg
     1501 agcaaactcc tgcgcctttg ccagcaggac atactgttga ttcagcgcca gaaatggcag
     1561 aacataatgt ccggcttcgt cggtgatggc ttcaactgcc tcaggagagt ctccccagaa
     1621 gccccctctg ccccttcctc tgcctcccgg acgggcgatg acgcgcgttt ccggtacggg
     1681 gcttctgccg aaagactgga agacgtgacc ggaaatggag ccacccggct ccaggtgaat
     1741 gaccaccggc tccttgcgat g
//
LOCUS       DAOQUC010000951         1758 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_89334_length_1758_cov_3.960070, whole genome shotgun sequence.
ACCESSION   DAOQUC010000951 DAOQUC010000000
VERSION     DAOQUC010000951.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1758)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1758)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1758
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89334_length_1758_cov_3.960070"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..402)
                     /locus_tag="PKH07_14390"
     CDS             complement(<1..402)
                     /locus_tag="PKH07_14390"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HNT36171.1"
                     /translation="MRFAICNETFQGWDFEHVCSFVSKTGYQGLEIAPFTLAESVCAI
                     SSSTRRQLRLSLERSGLQMVGLHWLLASPKGLYLNHPDREIRDRTVDYLRSLIQFCAD
                     LGGALMVFGSPKQRNVHPALNKTQAWQYAAEG"
     gene            complement(598..>1758)
                     /locus_tag="PKH07_14395"
     CDS             complement(598..>1758)
                     /locus_tag="PKH07_14395"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNT36172.1"
                     /translation="LHRIRTEEQIRASSRLEATATLAGGVAHDFNNLMVGVLGNAELL
                     RMDLADNAELQPMLNDIIEAAQRAGSLAQQMLAYARGGKYRPQVINLNKVVQETLNFQ
                     QRRVPPTVEIERRLEEDLWTINADPTQMSQVVMNLCNNAVDALGEQGLIIIGTENVEI
                     GFSAADAQHGVPPGRYVCLSVEDTGCGMSEEVRSRVFEPFYTTKFQGRGLGLAAVYGI
                     VSNHGGSLTVHSEEGVGTVFKVFIPATEMEVTATVPLEEGLPRGTETILLVDDEDLVL
                     KTTRNLLERLGYQVLVAQDGKQAVSIAQSHPREIHLAILDIGMPVMSGTDAYPLLAKA
                     RPGMKIIVCSGYDMKHTSETFAQQQGTFFVQKPFRASVLAPIVRRALSESGT"
BASE COUNT          416 a          481 c          452 g          409 t
ORIGIN      
        1 gccctctgcg gcatactgcc atgcctgggt cttgttcaat gcgggatgca cgtttctttg
       61 cttgggagaa ccaaagacca ttagtgctcc acccaggtcg gcgcagaatt ggatcagaga
      121 tctgagataa tccaccgtgc gatctcgaat ctcccgatcg ggatggttga gatagagtcc
      181 tttgggactt gcgagaagcc aatgaagacc gaccatctgc aagccagaac gctccagact
      241 gagacgcaac tgccttctgg tcgaggagct gatcgcgcag actgattctg ccagagtgaa
      301 gggagctatt tccaacccct gatatccagt tttggaaaca aaggaacaaa cgtgttcgaa
      361 gtcccaaccc tgaaaagtct cgttacagat tgcaaatcgc aatgaaatct ccttcaggca
      421 tagtctttct tagtagagcg atcttccgac gacgttgctt tccgctcata cagcaccttt
      481 gcaggactga agtcgcacca aattcccctt aatccaaggg ggattgagga ggattgggaa
      541 aacgagaaac tggttgtctt gcatattgta tggcaacggc acttcaggaa cgctccacta
      601 agtgccggat tccgacaggg ctcgacggac gatcggagcc aatacgctgg ctctgaaagg
      661 tttctgcacg aagaaggttc cctgttgttg ggcaaaggtt tccgatgtgt gtttcatgtc
      721 atagccgctg cagacgatga tcttcatccc cggccgtgcc tttgccagaa ggggatacgc
      781 atcggtacca ctcattaccg gcatcccaat gtccaaaatg gccagatgga tttcccgggg
      841 atgcgactgt gcgatggaaa ccgcctgctt gccgtcctga gctaccagaa cttgatagcc
      901 gagtctttcc agaagattcc gagttgtctt gagaaccaga tcctcatcgt ccaccaggag
      961 aatagtctcc gttcctctgg gaagaccttc ttccaaagga acggtcgcgg ttacttccat
     1021 ctctgtggca ggaataaata ctttgaagac tgttcccacc ccttcttcac tgtgaacggt
     1081 cagagaccct ccatgattgc tgacgatccc atagaccgct gccaatccca agcctcgccc
     1141 ctgaaacttg gtcgtgtaga aaggctcaaa aacacgcgag cgaacctctt cagacatacc
     1201 gcatcctgtg tcttcaacgg aaagacaaac ataccggcct gggggaacac cgtgttgcgc
     1261 gtcggccgca gagaatccta tctccacgtt ctccgtaccg atgataatca ggccttgctc
     1321 accaagagcg tccacggcat tgttgcagag gttcatgacg acttgactca tctgtgtcgg
     1381 atctgcattg atcgtccaaa ggtcttcctc cagacgacgt tcgatctcga cggtgggagg
     1441 aacacggcgc tgttggaagt tcaatgtctc ctgaaccacc ttgttgaggt tgatgacctg
     1501 agggcggtac ttacctccac gtgcgtaggc aagcatctgc tgggcgaggg atcctgcacg
     1561 ctgcgccgcc tcgatgatgt cgttcaacat gggttgcagc tcggcattgt cagcaagatc
     1621 catacggagc aattccgcat tgcccaacac ccccaccatc agattattga aatcatgagc
     1681 aacgcctccg gcgagtgtgg ccgttgcttc aagacgggat gaagcgcgga tctgctcttc
     1741 cgttcgaatc cggtgaag
//
LOCUS       DAOQUC010000952         1758 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_89384_length_1758_cov_2.320023, whole genome shotgun sequence.
ACCESSION   DAOQUC010000952 DAOQUC010000000
VERSION     DAOQUC010000952.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1758)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1758)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1758
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89384_length_1758_cov_2.320023"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            30..1658
                     /locus_tag="PKH07_14400"
     CDS             30..1658
                     /locus_tag="PKH07_14400"
                     /inference="COORDINATES: protein motif:HMM:NF014044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNT36173.1"
                     /translation="MTVDPDKIFGRHLAILGNTGSGKSCSVAGLIRWSLAHARRARTD
                     SGRPNARFIILDPNGEYSGAFADYDQVRRFQVSPTDANIKPLLIPVWMWNSQEWSAFA
                     HAAPGVQRPLLLQALRGMRSGARLHEPAERQAARLMYSYKTMIEGRISRGAGGYQGFP
                     ECKNCGNQIANLATDAQSHAGNTKGPFAQALLDVASAAEQVAAARYWKSANSDGYNDF
                     SETELRDVIDAIDVALATLPNQPTERLISEDAPIEFPSDSFPDHIEHIATEAPGGQTP
                     QFLATLIMRIRMLLADRRLGPVVNPGTGVISFAQWLESYIGNNQAENGELAILDLSLV
                     PSDVLQIIIAVVARIVFEATQRYRKLNDEQLPTVLVLEEAHTFVKRGSDEESDTPTPS
                     QMCRQTFERIAREGRKFGLGLVLSSQRPSELSPTVLAQCNTFLLHRIVNDRDQELVER
                     LVPDNLGGLLKELPSLPSRQAILLGWAATVPTLVEITELPEDHRPRSSDPEFWHVWTG
                     KKERPANWNEIVDDWTGIQSISTTPSGTSEEGNQ"
BASE COUNT          448 a          443 c          462 g          405 t
ORIGIN      
        1 gcacctcccc tctcgccgcc aacgcagacg tgacagtgga tccggacaag atattcggtc
       61 gtcatctcgc aattctcggg aacactggaa gtggtaaatc gtgctccgtc gctggtctga
      121 ttcgatggtc gttggctcac gccagaaggg cgcggactga ttctggacgc cccaatgcac
      181 gcttcatcat cctcgatccg aacggggagt attctggtgc ttttgcggac tatgatcaag
      241 tgcgaaggtt tcaggtctcc ccgacagatg caaacataaa accgttactg atccctgtat
      301 ggatgtggaa cagtcaagaa tggagcgcct ttgcccatgc tgcgccaggt gtgcagcgac
      361 ccttgttact tcaggcgctg cgcgggatgc ggtctggtgc acgcctccac gagcctgcag
      421 agcggcaagc agcaaggttg atgtactcat ataaaacaat gatcgagggg cgtatctcac
      481 ggggcgcagg tggctaccag ggcttcccag agtgcaagaa ctgcggtaat cagatagcaa
      541 acctcgcgac agacgcacag agccacgcag ggaatacgaa aggtccattc gcccaggctc
      601 ttttggatgt tgcttccgct gcggagcagg tcgccgcagc aagatattgg aagagcgcta
      661 atagcgatgg atacaatgac ttcagcgaaa ctgaactccg agatgttatt gatgctatcg
      721 acgttgcact ggcaacattg cccaatcagc cgacggaaag gctaatctcg gaagacgccc
      781 ccatcgagtt tccgagtgac tcttttccgg atcatattga gcatatagct accgaagctc
      841 cgggaggtca aacaccgcaa ttccttgcca ctctgattat gagaattaga atgctgttgg
      901 ccgatcgacg gcttggtcct gttgtgaatc cgggcacagg agtcatcagc ttcgcgcaat
      961 ggcttgagtc ttacatcggc aataaccagg ccgagaacgg cgaactagca atattggatc
     1021 tttctctcgt gccatcggat gtgcttcaga tcatcattgc tgttgttgct agaattgtgt
     1081 ttgaggcgac acaacggtat cgaaagctca acgacgaaca attgccgaca gtgttggtgc
     1141 tcgaggaagc gcatacattt gtcaagcgcg ggagtgacga agaatcggac acacccactc
     1201 cttctcagat gtgtcgtcaa accttcgaga gaatcgccag agaagggcgg aagttcggat
     1261 taggccttgt actctcatca caaagacctt ccgagctgtc gccaactgtt ttggcccaat
     1321 gcaatacatt ccttttacat cgtatcgtaa atgatcgcga ccaagagctt gttgaaagac
     1381 ttgtaccaga caatcttggg ggacttctga aagaattgcc aagtctgcct tccagacagg
     1441 caatacttct cgggtgggct gcgactgttc ctacactggt ggaaatcacc gagcttccgg
     1501 aagatcatag gccgcgatct tcagatccag agttctggca tgtctggaca gggaagaaag
     1561 aacgtcctgc taattggaac gagatagtgg atgattggac tggtatccag agtatttcaa
     1621 caacaccgag cggtacatct gaagaaggga accagtaatg attccaaaag aatgtaaacg
     1681 cctggctgaa gttgactttc ctatcgctat agtttcaaaa cactctatac gcgagaaacc
     1741 tattgtccac aatcatcc
//
LOCUS       DAOQUC010000953         1757 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_89432_length_1757_cov_3.598120, whole genome shotgun sequence.
ACCESSION   DAOQUC010000953 DAOQUC010000000
VERSION     DAOQUC010000953.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1757
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89432_length_1757_cov_3.598120"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..775)
                     /locus_tag="PKH07_14405"
     CDS             complement(<1..775)
                     /locus_tag="PKH07_14405"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNT36174.1"
                     /translation="MGKSEAVESSGKINRWHVAIAGVVMQLLLGSVYAWSVFKKPLME
                     SHGWSNSSVGLTFTFVNVFIGVSAAFGGRFVDKAGARKVATTGAILFGLGTLLAGLAD
                     KVGSIWLLWIGYGLIGGIGNGLGYVTPVAILVRWFPDKRGFITGLAVMGFGFGAALMG
                     KIAPLIIPHVGVANTFFLFGILYLICLLPAASRLNNPPATWTPPAPVQVKAKSDAVTV
                     SEDLPQALRMPQFYLLWGVLFINVTAGLSIISNLSPMGQI"
     gene            complement(1045..>1757)
                     /locus_tag="PKH07_14410"
     CDS             complement(1045..>1757)
                     /locus_tag="PKH07_14410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36175.1"
                     /translation="STPTPEISDTFSVPEFFPLVADSLWYYVGIPEEGGSAEDNFTWE
                     QKGTKAVGDKTATIIQTTTDESTDARNGDQDFWYVEAGTLYYKGYRNGTEKVIGSGFA
                     QIRIPPQDIVFSPGLAVGGTQKVGDSVSSAGTAQVNIVTFLGPSTVTANVSTNVTYDR
                     FDASVNTPLGQFMNVLHLTLSIDASVSGQPINLRGNEFWLKKNVGMVKQDQDPDPDDA
                     QVQGIESGQVGGQSIVAN"
BASE COUNT          437 a          506 c          420 g          394 t
ORIGIN      
        1 ggatttgtcc catcggagaa agattgctga taatggaaag tccggctgtc acattgatga
       61 aaagaacccc ccaaagcagg tagaactgtg gcatcctcag agcctgtggc agatcttcag
      121 aaacggtgac ggcatcagat tttgctttga cctgcaccgg agcaggcggt gtccaggttg
      181 caggaggatt gttgagcctc gaggctgctg gcagcaagca gatgagatac agaatcccaa
      241 agaggaagaa agtgtttgcc acgcccacat gagggatgat gagcggcgcg atcttgccca
      301 tgagcgcagc accaaagcca aaacccatga ccgcaagccc tgtgataaaa ccacgcttgt
      361 caggaaacca tcgaaccaga atcgcaacag gagtcacgta gccaagaccg tttcctatac
      421 caccgatgag cccgtagcca atccagagga gccagatgct tcccactttg tcggcgagac
      481 ccgccaagag agtcccgagt ccaaagagaa tcgcccctgt cgttgccact ttccgggcac
      541 cggctttgtc cacgaagcgc ccaccaaagg cggcagagac tccaatgaac acgttgacga
      601 aagtaaaagt tagccccacg gaggaattag accacccgtg gctttccatc aaaggcttct
      661 tgaagacgct ccaagcatag accgacccca gcaggagctg catcacaaca cctgcaatcg
      721 caacgtgcca cctgttgatc tttcctgacg attcgaccgc ttcgctcttg cccatttctt
      781 ctccttactc ttgtgaaaat tttcacattt tattggggga tgactcatgt gtagatatga
      841 gtccaaggta caccccatct cgacaaactg tagcattaga actttgccaa acccttgtgt
      901 caaggtaatt ctatggctct ctgacgtagg gatccataca gggctatgac atacttacaa
      961 acagactttc gggaacgtcg taccccagac aaccgccagc ctggtcgagt tacctgccac
     1021 acaggacgat ctggcgaccg cccctcaatt cgccacaatg ctctgaccgc caacctgacc
     1081 gctttcgatc ccttgcacct gggcgtcgtc aggatcgggg tcttgatctt gcttgaccat
     1141 gccaacattc ttcttgagcc aaaactcatt gcctctcagg tttatcggct gccccgaaac
     1201 actcgcatca atacttagcg tgagatgcaa tacattcatg aactgtccca aaggcgtatt
     1261 cactgatgcg tcaaaacgat cataggtgac gttggtgctc acattcgctg tgacggtgct
     1321 gggaccaagg aaagtgacaa tgttgacttg cgccgttcca gcacttgaga cgctgtcacc
     1381 aaccttctgc gttccgccca cggcaagacc gggactgaac acaatatcct gaggtggaat
     1441 cctaatctgt gcgaatcccg acccgatgac cttctcagtt ccgttccggt agcccttgta
     1501 ataaagcgtc cccgcttcca cataccagaa atcctgatca ccatttcgag catctgtgga
     1561 ctcatccgtt gtcgtctgga tgattgtcgc cgtcttgtct ccaacagcct tggttccctt
     1621 ctgttcccaa gtgaagttat cctctgcact tccaccctct tcggggattc cgacatagta
     1681 ccagagactg tctgcgacca gcggaaagaa ctcgggaacg ctgaaggtat cagagatctc
     1741 tggggtggga gtgctcg
//
LOCUS       DAOQUC010000954         1757 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_89451_length_1757_cov_2.768508, whole genome shotgun sequence.
ACCESSION   DAOQUC010000954 DAOQUC010000000
VERSION     DAOQUC010000954.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1757
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89451_length_1757_cov_2.768508"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1113
                     /locus_tag="PKH07_14415"
     CDS             <1..1113
                     /locus_tag="PKH07_14415"
                     /inference="COORDINATES: protein motif:HMM:NF039847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNT36176.1"
                     /translation="MGFCAYPEVQVVAVCDVDAPRCEGAKRQVDAVYGNQDCACIHDF
                     REIIQRRDIDIVCIATPDHWHAIPAIWAMRSGKDVFVEKPLSLTIAEGRALVNTAKEC
                     GSVTQVNTWQRSIHNFQHACDLIQGGRLGRVHTAKVGLPGGPSQPPQPEVPVPEGFDY
                     DFWLGQAPWAPYHPSRCHGAFRACFDYSGGGLTDWGAHHLDVVQWYMNRDSTGPVKIE
                     GTGEFPTEGIWNTATSYHFICTYDDGFTLEASTSFRGGVRFEGEDGRWLHVDRGVLET
                     NPADLRKQRIAPDEMKQLWVSGDHNADFLNSIRTRRPTVTPVEIGHRSISIAHLANIC
                     MRLGRSLRWDPAQEHFIDDPSADRMLSRAMRQPWHL"
     gene            1175..>1757
                     /locus_tag="PKH07_14420"
     CDS             1175..>1757
                     /locus_tag="PKH07_14420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015814693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNT36177.1"
                     /translation="MKKEKQKDLGTTRRDFLVAAGLGSLALGIGAKRMEAAEVTENKQ
                     EVIQGFDDTQTSTDTSKKWVQTHDRKIRVGIAGFGVCKFGAAFGFQDHPNVEIVAVTD
                     LFPDRCAGLAQACRCDKMYPSLEEMVKDDKIEAVFVATDAPSHARHCLEVLKHGKHVC
                     TACPAAFGSVEDAEQVLAAVKQTGLNYMMLETTT"
BASE COUNT          407 a          433 c          516 g          401 t
ORIGIN      
        1 atgggattct gtgcgtatcc ggaagtccag gttgtcgccg tctgcgatgt ggacgctcct
       61 cgatgcgaag gtgccaagag gcaagtggac gctgtctatg gaaatcagga ctgtgcttgc
      121 attcatgact ttcgagagat catccaacga cgagacattg acatcgtttg catagcaacg
      181 ccggatcatt ggcatgccat tccagcgatc tgggcaatgc gttccggcaa agatgtcttt
      241 gtcgagaagc cgttatccct gaccatcgca gaaggtcggg ctctggtgaa tacggccaaa
      301 gagtgcggtt cggtcactca ggtcaacaca tggcagcgct cgattcacaa ctttcaacac
      361 gcctgtgatc tgattcaagg tggccgcctg ggtcgcgtcc atacggccaa agtcggactt
      421 cccggagggc cttctcagcc gcctcaaccc gaagtgccgg ttccggaggg ttttgactat
      481 gacttctggt tgggacaagc gccttgggca ccgtatcatc catctcgctg ccatggtgcc
      541 ttccgtgcct gctttgacta ttcaggcggg ggcctgaccg actggggagc ccatcatttg
      601 gatgtcgttc agtggtatat gaaccgagac agcacaggcc cagtgaagat tgaaggcaca
      661 ggtgaatttc caacagaggg catctggaac acggcaacaa gctatcattt catctgcacc
      721 tacgacgatg gatttacttt ggaagcctcg acaagttttc gtgggggtgt ccgttttgag
      781 ggcgaggatg gacgttggct tcacgtggat cgaggtgttc ttgaaacaaa tccggcggac
      841 ttgcgtaagc agcgcatcgc tcccgatgag atgaagcagc tttgggtcag tggtgaccac
      901 aacgcagatt ttctcaacag catcagaacg cgcagaccaa cagtcactcc agtagagatc
      961 gggcatcgtt caatctccat tgcacacctt gcaaacattt gtatgcgatt gggcagaagt
     1021 ctgcggtggg atcccgcaca ggagcacttc attgatgatc cctcagcgga tcggatgctg
     1081 tcacgagcca tgcgtcagcc gtggcatctc tgatggcgac tgatgaggtt atacagtctt
     1141 ggatttctta tggagaatgc atggaggtgg cataatgaag aaagagaagc agaaagattt
     1201 gggcaccaca aggcgtgact ttcttgtcgc ggcagggctg ggatctttgg ctctgggcat
     1261 tggtgcaaaa aggatggagg cggcagaagt gaccgaaaac aagcaggaag tgattcaggg
     1321 tttcgacgac acacagacca gtacggacac atcgaagaaa tgggttcaga cgcatgacag
     1381 aaagattcgt gttggcatcg caggcttcgg cgtctgcaag tttggtgcag ccttcggatt
     1441 tcaggatcac cccaacgtgg agatcgttgc ggtgactgat cttttccctg atcggtgtgc
     1501 aggccttgct caggcatgcc gctgcgacaa gatgtatcca tcgctcgaag agatggtcaa
     1561 agatgacaag atcgaagcgg tcttcgttgc cacggatgcg ccaagccatg cccgccactg
     1621 tcttgaagtc ctcaagcatg gtaagcatgt ctgcacggct tgtcccgctg cttttgggag
     1681 cgttgaagat gcagaacagg tgctcgcggc ggtcaagcag acggggctga actacatgat
     1741 gcttgagacc acaacct
//
LOCUS       DAOQUC010000955         1757 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_89475_length_1757_cov_2.273796, whole genome shotgun sequence.
ACCESSION   DAOQUC010000955 DAOQUC010000000
VERSION     DAOQUC010000955.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1757
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89475_length_1757_cov_2.273796"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="PKH07_14425"
     CDS             <1..207
                     /locus_tag="PKH07_14425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36178.1"
                     /translation="ARVSRGYTIFYENMMVSRIRQRDKWWEVLWWSHRDKWESIGDMG
                     GVLFDTVEEAATYVLDDPMGIFWR"
     gene            complement(290..577)
                     /locus_tag="PKH07_14430"
     CDS             complement(290..577)
                     /locus_tag="PKH07_14430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36179.1"
                     /translation="MVDTPAHSAKPQVKRAPSTPSAVVSAQEAQEAIRAAFPYEGDLR
                     VRHLWSGNGVARYRANWFRQVEGEMKVVTSLFIGITRTADGLVVQNETAVG"
     gene            1013..>1757
                     /locus_tag="PKH07_14435"
     CDS             1013..>1757
                     /locus_tag="PKH07_14435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNT36180.1"
                     /translation="MKCVWLSDVHLNFLKKSAFIGFLRTLQEHEADAVLLSGDIAEAH
                     RVCDYLFMIDVALQTKIYFVLGNHDFYRGSIIRTRQAVAELVQDSRYLVYLTQVGVVQ
                     LTPTTCLVGHDSWADGRLGDIERSDVEMNDFYLIEELAGLDSEERRDKLRELGEEAAD
                     HLRRVLTEAVKSYRDIFVVTHVPPFREACIHEGRISDDNYLPHYSCHSAGEVLRDTAT
                     SWPDHRFTVFCGHTHSAGQVRILDNLEVYT"
BASE COUNT          392 a          483 c          498 g          384 t
ORIGIN      
        1 gcccgagtca gtcggggcta tacgatcttc tacgagaaca tgatggtctc ccgcattcgc
       61 cagcgcgaca agtggtggga agtcctgtgg tggagccatc gcgacaagtg ggaatccatc
      121 ggcgatatgg gcggtgtttt gttcgacacc gtggaggaag cagcgactta cgtcctggac
      181 gacccgatgg gcatcttctg gagatgaccg gaatgaagga tcacacaccc gtcatccgac
      241 tgggcaaaga gaaaggtcaa tcggctcata aacatgaccg tccacagcct catcctacgg
      301 cagtctcgtt ctggacaacc agaccgtccg ctgttctcgt gatgccgatg aacaatgacg
      361 tgacaacctt catctcgccc tccacttggc ggaaccaatt cgcgcggtac ctcgcgaccc
      421 catttcccga ccagagatgg cgaacccgga gatcaccttc atatggaaaa gctgcgcgaa
      481 tagcctcttg tgcttcttgg gcggacacga cggcggaagg agtgcttggt gctctcttga
      541 cctgaggttt tgcgctgtgg gcaggcgtgt caaccatggg tacctcctgg ttaccgagtt
      601 cacaaccgga acagactcgt caacttgtga tcattcagca gatgccgtgc cagatcacca
      661 cagtaactgc ctggggtggt ccacagcgcg taagccgtta tcaggtgtgg tcttggcaaa
      721 cagcgacgat aagcgggtac cgcattcgag cggccaatct ggcatcgtgt tttggcagcc
      781 tgtgcatgaa tagtcagggc aatggggtcg gatgacgtag acgacagagt agggtaatcc
      841 gcgttctctt ctccgccgaa gctggacatc ggttttcact ttcgggcgga aaaagaaagc
      901 tacccgtcat ttaccgatat acctactgcc cacgaaggtc accggctgat gccgaaaacc
      961 gtgggcagtt gggcacttgc cgtaacaggc cggatacgga cgagcaacga acatgaaatg
     1021 tgtttggctc agcgatgttc acctcaactt tctcaagaag agtgcgttca tcggtttcct
     1081 tcgcaccctc caggaacacg aggccgacgc cgtcctcctt tcgggcgata tcgcagaagc
     1141 tcacagagtc tgtgactacc tcttcatgat cgatgtcgcc ctccagacaa agatctactt
     1201 tgttttggga aaccacgatt tctaccgtgg ctcgatcatt cgtacgaggc aggctgtggc
     1261 ggaattagtc caggactctc ggtacttggt ctacctgacc caggtcggcg tcgtgcagtt
     1321 gactcccaca acgtgccttg tgggacacga ttcctgggca gacggcagac tgggcgacat
     1381 cgagcgctcg gatgtcgaga tgaacgactt ttacctgatc gaggaacttg ccggactgga
     1441 tagtgaagag cgacgagata agttgcggga actcggggag gaggctgcgg accatctccg
     1501 cagagttctg actgaggctg tgaaatcgta tcgagacatc ttcgtcgtaa cccatgtgcc
     1561 tccgttcaga gaggcgtgta tccatgaagg acgcatcagc gatgacaact acctgccgca
     1621 ctactcctgc cactccgcag gcgaagtctt gcgagatact gccacgtcgt ggcccgatca
     1681 ccgattcacg gtgttttgcg gtcatactca cagcgcgggc caggtacgca tccttgataa
     1741 ccttgaggtc tatacgg
//
LOCUS       DAOQUC010000956         1756 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_89523_length_1756_cov_3.352734, whole genome shotgun sequence.
ACCESSION   DAOQUC010000956 DAOQUC010000000
VERSION     DAOQUC010000956.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1756
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89523_length_1756_cov_3.352734"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="PKH07_14440"
     CDS             complement(<1..300)
                     /locus_tag="PKH07_14440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020877883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36181.1"
                     /translation="MVTQTKLHPRFEKLLRYAMSQGASDLFLIPGEPPALRVRGLIER
                     TEDEILTADEISEFAREAVGEETLTRVGTEVGEIHRSCSLTGEANARMSIASCCGQ"
     gene            complement(356..907)
                     /locus_tag="PKH07_14445"
     CDS             complement(356..907)
                     /locus_tag="PKH07_14445"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNT36182.1"
                     /translation="MIESLREILTRCMAKDDEAVAELVRRFRPWALDFVTALLEQTAL
                     AEDVVQEGFVAALTKLPNLRDLDAFPGWFRQILRTLVSRANRRKKEIQWNDKIQYPSY
                     ALTPREQLDKDDLRKRLLGILQSLPATTRETAELFYIREMKNVEISQYLKTPLGTVKR
                     RLHDARRTLRARLMERLDGSVSR"
     gene            complement(1115..>1756)
                     /locus_tag="PKH07_14450"
     CDS             complement(1115..>1756)
                     /locus_tag="PKH07_14450"
                     /inference="COORDINATES: protein motif:HMM:NF024716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HNT36183.1"
                     /translation="SYFHSADEPDGPEQVADYRKARELLREFAPWMKVIDAMSDTHFA
                     VERLTDMPIPSIATAPEFTKADCPAWTYFCCGPRGGYLQRLHDTPLPKIRMAGWLFYR
                     LGAKGFLHWGHNYWYVFCTGTIADPYTDPAVGAYPGLPYGDPFVVYPGANGPVDSIRW
                     EVFAESLQDYALLQSAGIGPDDPLLDSLKDYQDFPKSEKWLADARESILKKKK"
BASE COUNT          384 a          477 c          451 g          444 t
ORIGIN      
        1 ctgtccgcaa caggaggcga tactcattct cgcattggct tcccctgtca gtgagcagga
       61 gcgatggatt tctccgactt ctgtccctac acgtgtcagg gtttcctcac cgacggcctc
      121 tctggcgaat tcgctaatct catcggcagt cagtatctca tcctccgttc tctcaatgag
      181 tccacggacg cgcaacgctg gtggttcacc tggaatcagg aacaagtcgc tcgctccctg
      241 gctcatcgcg taacgaagca gtttctcgaa tctggggtgc agttttgtct gagttaccat
      301 gtcacatcct ccttattgga actaacacaa atatcccttg gctgaattcc aactcctacc
      361 tcgaaactga accatccagg cgttccatca ggcgagcgcg gagtgttctc ctggcatcgt
      421 gcaagcgtct cttgacagtg cccaatgggg tcttcaggta ttgagagatc tccacattct
      481 tcatctcgcg aatatagaac agctctgccg tttctcgagt tgtggcggga agcgactgaa
      541 gtatcccgag caaacgtttc cgcagatcgt ccttgtcgag ttgctctctg ggtgtcaacg
      601 catagctggg atactggatt ttgtcgttcc actgaatttc ctttttcctt ctgttggctc
      661 tggagacaag agtccttagt atctgtcgaa accaaccagg aaacgcatcc aaatcccgca
      721 ggttgggcaa ctttgtcaaa gctgcaacaa agccctcttg aacgacatct tcagccagtg
      781 cagtctgttc cagaagggct gtcacgaaat ccagtgccca aggtctgaag cgtcgaacca
      841 attccgccac tgcctcgtca tcctttgcca tgcagcgggt cagaatttcc ctgagcgact
      901 cgatcattgt gcttcctcat ctgtctttct gaatcataag gaaccctgtt gaggtgaaaa
      961 ggttcggaga aagatatgga ttgctaccgg tccgtgctgt cagggtgcat ctggttactc
     1021 aagacggtgc gagtcctcga cgtttttggg tatgccggat accttctttc tctctccaag
     1081 aactctttga agtcgggatg gagttcgcgc atcctcattt cttcttcttc agaatgctct
     1141 cccttgcatc ggccagccac ttttcggatt ttgggaagtc ctggtaatct ttgagactgt
     1201 cgagaagtgg atcatcggga ccgataccag cagactggag aagtgcgtaa tcctgaaggc
     1261 tttcggcaaa gacttcccat cgaatcgaat caacgggacc atttgcacca ggatagacga
     1321 caaatggatc gccgtagggc agtccgggat aggctcccac ggcgggatcg gtatacggat
     1381 ccgcgatggt gccggtgcag aagacgtacc agtagttgtg tccccaatgc agaaaaccct
     1441 tggctccaag tcggtagaag agccatcctg ccatacggat ttttgggagg ggtgtgtcgt
     1501 gaagtcgctg caagtatcca cccctggggc cgcaacagaa atatgtccat gccggacaat
     1561 ctgccttggt gaactccggt gcagtggcga tgcttgggat gggcatatcc gtgagccttt
     1621 ccacggcgaa gtgcgtatcg ctcatggcgt caatgacctt catccaagga gcgaactcac
     1681 ggagcaactc cctcgctttc cgatagtcag caacctgctc gggaccatcg ggctcgtccg
     1741 cggaatgaaa atagga
//
LOCUS       DAOQUC010000957         1756 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_89526_length_1756_cov_3.272781, whole genome shotgun sequence.
ACCESSION   DAOQUC010000957 DAOQUC010000000
VERSION     DAOQUC010000957.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1756
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89526_length_1756_cov_3.272781"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..708
                     /locus_tag="PKH07_14455"
     CDS             <1..708
                     /locus_tag="PKH07_14455"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HNT36184.1"
                     /translation="DAFGRAVPASTPWEPMSSFAPKEHLTGKMYDDMTGLYYFHARWY
                     DPELGRFVSRDPVTSHDSGPHVFCDSNPTNLLDPSGEKVSDSFEYDSCCDPIFQREGI
                     SKKAILKELEAWISTWLVRWQPKYPRESACMFASKIKITCVDGTHTQTRLLCGDIPPW
                     SSEATLYFDNHKHRTPEGVAHTSIHEKSHMCGWGWLLKQHPIKNPWRSVPCAYFPDEG
                     SISCDNQMPAEDHPTYR"
     gene            732..1208
                     /locus_tag="PKH07_14460"
     CDS             732..1208
                     /locus_tag="PKH07_14460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36185.1"
                     /translation="MKKRLLRTAFIIFLVVLPIVLQHSVFIGQLMNGDHYIRYQINGV
                     VCGKHDGEPLPETTIRVHPDYRPSLCLATVVSSATGFFSDEGWMGGSPPRLTGLLARE
                     YEWTHHGRRGLWIDLEKADFEEVSKFVHFGSCTYDHPLYGWVWIAEAGRVRLRRAY"
     gene            1258..1632
                     /locus_tag="PKH07_14465"
     CDS             1258..1632
                     /locus_tag="PKH07_14465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36186.1"
                     /translation="MQLWSILVTSSIAALLALLAFRSKKSICALSILGLALMGCCRTT
                     AFFFSLIPLSSILLWPQNGKVVLMGALAISLLYCSFVLLSRYKEAQADWLSHRVLRIM
                     VLWYFTVGVLYGLFLGIFPLSR"
BASE COUNT          386 a          409 c          470 g          491 t
ORIGIN      
        1 gacgccttcg gccgggctgt gcccgcgtcc acgccttggg aaccgatgtc atccttcgcg
       61 cccaaagaac acctcacggg caagatgtac gacgacatga ccggtctcta ctacttccac
      121 gcccgctggt atgatccgga actggggagg tttgtgagtc gggatccggt caccagccat
      181 gattcgggtc cgcatgtgtt ctgtgattcg aacccgacca accttcttga tccgagtggc
      241 gaaaaggtat cggacagttt tgagtatgac agctgttgtg atcccatctt ccagagggaa
      301 ggcatttcaa agaaggcaat actcaaagaa cttgaggcct ggatcagcac atggttggtc
      361 agatggcagc ctaagtatcc cagggagtcc gcatgcatgt ttgcttcgaa gatcaagatt
      421 acctgtgtgg acggaacgca cactcaaaca aggcttctat gtggagatat ccctccttgg
      481 agcagtgaag caactctcta ttttgacaac cataagcaca ggactcctga gggtgttgca
      541 catacttcta ttcacgaaaa atctcacatg tgcgggtggg ggtggcttct gaaacagcat
      601 ccaatcaaaa acccttggcg cagcgttcca tgcgcctatt tccctgatga aggttctata
      661 agttgtgaca accagatgcc agctgaagat catccaacgt acaggtgaac cttcccaagt
      721 gtcagaatga gatgaagaag aggcttctca gaactgcgtt catcatcttt cttgtggtgc
      781 ttccgattgt tcttcaacac tcggtattta ttggtcagtt gatgaatgga gaccactaca
      841 tccgttatca aatcaatggg gttgtctgcg gcaagcacga tggagaacct ctacctgaga
      901 cgacaatacg cgtgcatcct gattatagac cgtctttgtg tctggcaaca gtcgtgagtt
      961 cagcaaccgg ttttttcagt gacgaaggtt ggatgggtgg gagtcctcca cgcttgactg
     1021 gtttgcttgc tcgcgagtat gagtggacac accatggaag acgagggctg tggatagact
     1081 tagagaaagc cgactttgaa gaagtttcca agtttgtcca ttttggctca tgcacctacg
     1141 accacccact ctatggttgg gtttggattg ctgaagcagg ccgtgtgcgg ctgaggcgag
     1201 catactgaga gcgagccgga tgaggtttct gcaagtctct tgttgaagga gtttctcatg
     1261 caactctgga gtattcttgt cacttcaagt attgcagcgt tgttggcatt acttgcgttt
     1321 aggtctaaga agtcaatctg tgcgttaagc attttggggc ttgccttgat gggatgctgc
     1381 cgcacaactg catttttctt cagtctcatt cccttgagtt ccattctgct ttggcctcag
     1441 aatggaaagg tcgtcttgat gggggcgctg gcgatatcgc ttctttactg ttcatttgtc
     1501 ttgctttctc gctacaagga ggcacaggct gactggctgt cgcatcgggt gttgcgtatt
     1561 atggttctgt ggtatttcac agtgggtgtg ctgtatggtt tgttcttggg gatattccca
     1621 ttgagccgtt gatccagttg agtggtctct tcgcaaggta tccgggtagc gactgcgttg
     1681 agaatcaagc aatatatttc tgctccaacg tgccaggtgc cctctctttc ccgtgtggta
     1741 gccttcaact tgttga
//
LOCUS       DAOQUC010000958         1755 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_89626_length_1755_cov_3.093529, whole genome shotgun sequence.
ACCESSION   DAOQUC010000958 DAOQUC010000000
VERSION     DAOQUC010000958.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1755)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1755)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1755
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89626_length_1755_cov_3.093529"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1755
                     /locus_tag="PKH07_14470"
     CDS             <1..>1755
                     /locus_tag="PKH07_14470"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNT36187.1"
                     /translation="VTVMCEPPSERVGRPDFNQIMQMIKTVLVREGQETTDVDFQFKL
                     KIDDEQETTAEETPEPDKEEKNSYIRGVVVNSAGKPVPNVQIQLTPASLRRGFEGNIG
                     WHRTDQQGRFKIEKIDPGKYLLNISATGRYGATTVSADAPQENLTITLKGLGAISGRV
                     ILKTDKRPVTRFSLLCTPQTQNPMDGTRWLQGNKGTDYYSPRGDFLVENLAADRYELT
                     VRVTGYANGTAERVEVEEGKTTANIEIEIGSGGSITGIVVTGQDEEPVGGAKVTLDRT
                     GGNPGLALLGITDSDERSVLTDSGGYFELGNLNPGEAILLVSHPDYAPARSQSLRVVD
                     GEESASVIISLTAGGAIEGYVYGEDGKGLSGQLVTAMVMERGISVKAITDEKGCFRIT
                     GLEPSSYSVMLGDFTQMFFNRPGVSTKTAVVKEGETTVVNFGAQGAMVYGVVTRRGEP
                     VSAAKVAIRVSDMPFGFDFNANAVTDQRGYYQVQGLEKGHYFVMLSEGMLGIQALTKK
                     QIDIPESAGAYEVNLAYPESVVAGVVISATDQSPVRNASISLVPEEGGYAEQMAQMFG
                     GFTDTTNATVTKMIDIIRT"
BASE COUNT          420 a          429 c          535 g          371 t
ORIGIN      
        1 ggtcacggtg atgtgcgagc ctccttccga acgcgtagga cggcctgatt tcaaccagat
       61 catgcagatg atcaagacgg ttttggtgcg ggaaggtcaa gagacaacgg atgttgattt
      121 ccaattcaaa ctgaagatcg acgatgaaca ggaaacgaca gcggaagaaa cacctgaacc
      181 ggacaaggaa gaaaagaact cgtatatacg tggcgttgtg gtcaactctg ccggcaagcc
      241 tgtccccaac gttcagattc agctgacacc ggcttctttg cggcgaggat ttgagggcaa
      301 tatcggctgg cataggaccg atcagcaggg cagattcaag atcgagaaga tcgatccagg
      361 aaagtatctg ctcaacattt ccgccaccgg tcgttacggt gccaccacgg tgagcgccga
      421 tgctccgcaa gagaatctga ccatcacgct caaaggtctg ggtgcaatct cggggcgggt
      481 catcttgaag acggacaaga gaccggtgac tcgtttctcc cttctctgta ctccccagac
      541 gcagaatccc atggacggca ctcgatggct tcaaggcaac aagggtaccg actattactc
      601 gcccagaggg gatttccttg tggagaactt ggccgcagat cgctatgaac tcacggttcg
      661 ggtgacgggt tatgccaatg gaaccgcaga acgtgtcgaa gtggaagagg gaaagacgac
      721 agcaaatatc gagatcgaga ttggaagtgg tggaagtatc accggtatcg tcgtgacggg
      781 tcaggatgaa gaaccggtag ggggtgccaa ggtcactctc gaccggacgg gtggtaaccc
      841 cggattggcg ctgctcggca tcacggattc cgacgaacgc tctgttctca cagacagtgg
      901 tggttatttc gagctcggaa atctcaaccc gggagaggcg atcctgctgg tcagtcatcc
      961 ggactatgca ccggcgcgaa gccagagtct ccgtgttgtt gacggtgagg agagcgcttc
     1021 tgtgatcatc agtctgaccg caggtggagc catcgaaggc tatgtctatg gagaggacgg
     1081 caaaggactc tcgggacaac tggtgacggc gatggttatg gagcggggga tctctgtcaa
     1141 agcgatcact gatgagaagg gatgcttccg catcaccggt ttggagcctt ccagttattc
     1201 cgtgatgtta ggggatttca cgcagatgtt ctttaacagg ccgggtgtct ccaccaagac
     1261 cgccgtcgtc aaggaagggg aaaccacggt tgtcaacttc ggcgcgcagg gagccatggt
     1321 ctacggtgtg gtgacacggc gtggagagcc ggtatcggcg gccaaggttg ccattcgtgt
     1381 gagtgacatg ccttttggtt ttgatttcaa tgcaaacgcc gttaccgacc agcgcggtta
     1441 ctaccaggtt caggggcttg agaaaggaca ctacttcgtg atgctttcag aggggatgct
     1501 cggcattcag gctttgacca agaagcagat tgacatcccg gagagtgctg gtgcctatga
     1561 ggtcaatctg gcctatccgg aatcggttgt cgcaggcgtt gtcatcagcg ccaccgatca
     1621 gtcgcccgtt cgaaatgcct ctatctcgct ggttcctgaa gaaggaggat acgccgaaca
     1681 gatggcacag atgttcgggg gattcaccga cacaacgaat gcgacggtta ccaagatgat
     1741 cgatatcatc cgtac
//
LOCUS       DAOQUC010000959         1752 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_89906_length_1752_cov_2.308191, whole genome shotgun sequence.
ACCESSION   DAOQUC010000959 DAOQUC010000000
VERSION     DAOQUC010000959.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1752)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1752)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1752
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89906_length_1752_cov_2.308191"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..801)
                     /locus_tag="PKH07_14475"
     CDS             complement(<1..801)
                     /locus_tag="PKH07_14475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36188.1"
                     /translation="MLRLTTARRTLLLTSFFLLVLSADANIWVDESFEDGLAFDPTAL
                     DTYHPYGVGGPNVNPTHTGSVSTAKALLGTHSYQLDAGEVISVNEPYGNQANGPFQYF
                     QFGVNVDEIPAPGSMAKFQWNWTFLSKSHRFFVDFVSTGSEVQLWAGEDIADGTVSRS
                     FLLDTIGDTATWKYLTIQVQKNIPDATEEQIPGQLPLPQGAYFYCSSYSPQGVVPLYS
                     EIDLGEKAHGWSLSVTSGRLLVDEVYWEGGLGYDENRNLRPLSLQSLPT"
     gene            complement(903..>1752)
                     /locus_tag="PKH07_14480"
     CDS             complement(903..>1752)
                     /locus_tag="PKH07_14480"
                     /inference="COORDINATES: protein motif:HMM:NF024770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNT36189.1"
                     /translation="MSELIRGIDEEVFELGGHINLCLSNDSYEREAEILIRLTEQGTR
                     RILLFMVLSNEFEALNPNLPLYLNLQERGVRLLLMDCSLPHVAIPSIRWDDHGGMFKL
                     VAHMAERGCRHLAYIGRRNNATTTSNRYQGFKDALLEYGLPFDASRVKWASTNGTPSL
                     TEKSRDAAIAWFQKPQTVDAVLCSDEEVAAGVFEALEVSPNVSPDRMVIGGFGSAKSG
                     ILAKNDRYVLLDQDTYLLGKIAAEAVVEKSGESDTPARWGFQRVLDVPIRIPRKSPAL
                     RTNRRS"
BASE COUNT          432 a          456 c          429 g          435 t
ORIGIN      
        1 agttggcaga gactgcaaac ttaggggacg aagatttctg ttctcgtcgt agccgagtcc
       61 accttcccag tatacttcat ccacaaggag tcgcccactt gtgaccgaaa gagaccagcc
      121 atgtgccttc tctccaaggt caatttcgct gtacaggggc acaactccct gaggggaata
      181 actcgagcaa tagaaatagg ctccctgtgg cagaggcaac tgccctggaa tctgttcctc
      241 cgtggcatca ggaatgttct tctgtacttg gatggtcagg tacttccatg tggccgtatc
      301 cccaattgtg tcgagcaaga aggatcggct gactgtaccg tctgcgatgt cttcaccagc
      361 ccatagctgt acttcgcttc ctgtcgagac aaagtccaca aagaagcgat gggactttga
      421 cagaaatgtc cagttccact gaaactttgc cattgatcct ggcgctggga tctcatccac
      481 attaaccccg aactggaagt actgaaaagg cccattggct tgatttccgt aaggttcgtt
      541 cacggaaatg acctcaccgg cgtccaactg atatgaatga gtgcctagaa gagcctttgc
      601 agttgaaaca gaaccagtat gtgtaggatt tacattcggc cctccaacgc cgtaggggtg
      661 ataggtatcg agtgctgtcg ggtcaaacgc gagaccatcc tcgaaactct catccaccca
      721 gatgtttgca tcagcggaga gaaccaacag gaaaaatgat gtcagcagga gtgtccgtct
      781 agcggtagtc aatcgaagca ttttcttcct cctaatccaa ggaaagtagg ttggcattat
      841 tattgtcatc taatgtccct gtgtcaagca cattaagcac attcacagtc atagtgtata
      901 tatcaactcc gtctgttagt ccgtagcgcg ggagactttc tgggtatcct tattggaaca
      961 tcgagaactc tctgaaaacc ccatctcgca ggagtgtctg actcgccaga tttctccaca
     1021 actgcttctg ccgcgatctt cccaaggaga tacgtgtcct gatcgagaag aacatagcga
     1081 tcattctttg cgagaatacc gctctttgca ctgccaaaac cgcctattac catccgatcg
     1141 ggactcacat tgggggatac ttccagagcc tcaaagacgc cggcagcgac ttcctcatca
     1201 gagcataata cggcatccac ggtctggggt ttctgaaacc acgcaatcgc agcgtctctg
     1261 gatttctctg tcagagaggg agtgccattt gtggaagccc acttcacgcg ggatgcgtcg
     1321 aagggcagtc catactcaag taaggcatct ttgaatccct ggtagcgatt gctggttgtt
     1381 gtggcgttgt tccttctccc gatgtatgcc agatgtctac accctctctc ggccatgtgt
     1441 gccacgagtt tgaacatacc accatggtcg tcccagcgga tggatgggat cgccacatgg
     1501 ggaagagaac agtccatgag aagaagcctg acgcctcttt cctgaaggtt gagatagaga
     1561 gggagattcg ggttcaatgc ctcaaactca ttgctcaaga ccataaagag gagaatcctt
     1621 cgggtgccct gctccgtaag ccgaataagt atctcagctt ctcgttcgta cgagtcgttt
     1681 gaaaggcaga ggttgatgtg tccgcccagc tcaaacacct cttcatctat gcctcgaatg
     1741 agttcactca tg
//
LOCUS       DAOQUC010000960         1749 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_90107_length_1749_cov_3.316411, whole genome shotgun sequence.
ACCESSION   DAOQUC010000960 DAOQUC010000000
VERSION     DAOQUC010000960.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1749
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90107_length_1749_cov_3.316411"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..415
                     /locus_tag="PKH07_14485"
     CDS             <1..415
                     /locus_tag="PKH07_14485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HNT36190.1"
                     /translation="LPEESLPLMRVLHRAIEQNLVRSCHDLSEGGLAVAAAEMALAGR
                     IGMSLDLRSMPEHSSIEQDAALLFSESNGRFLVEVSHSQQQKFERLMSGLPCALLGET
                     VEDASLKVVGRTGEVAIEVALDDIERSWKRSLWTS"
     gene            517..1320
                     /gene="purQ"
                     /locus_tag="PKH07_14490"
     CDS             517..1320
                     /gene="purQ"
                     /locus_tag="PKH07_14490"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012984162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase I"
                     /protein_id="HNT36191.1"
                     /translation="MRQKKEIRAAVLRAAGTNCDYETGYALTLAGARPVLVHVRELLD
                     GIKKLNDYHLLIIPGGFSYGDDLGAGKLLANEVRYLLGEEMTRFVKAGKPVLGICNGF
                     QVLVKAGFLPEFQPGEWSQEMSLVANDSARFECRWTFLKPNPKSPCVFTQGITEPIPV
                     PIAHGEGKVVFRDALIQEQVVNNNLVAFQYVDSEGNAGPYPVNPNGSAWDIAGICDPT
                     GRILGMMPHPERHVHQWQHPWWTRRRHNEARGAGLRIFENAVQLCKDSL"
     gene            1435..>1749
                     /locus_tag="PKH07_14495"
     CDS             1435..>1749
                     /locus_tag="PKH07_14495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HNT36192.1"
                     /translation="MGEYVRKPDFQRLRKTLLLQGEADCVPLAELGIDNSMKEFVLGR
                     PVETLQDEVDFSKVAGYDYVKLAPGIDLNPANIQPKEGSRAADATQHDSARVFANEGQ
                     GII"
BASE COUNT          399 a          419 c          506 g          425 t
ORIGIN      
        1 tcttcctgaa gagtctctgc ctctgatgcg cgttttacat cgggcgatcg aacagaatct
       61 ggttcgttcg tgccacgatc tgtctgaagg cggactggcg gtggctgctg ctgagatggc
      121 gctggcgggt cgaatcggca tgagtcttga tttgcgatcc atgccggagc actcgtctat
      181 cgaacaggat gcagcactct tgttctcaga atcgaatggt cggttcctgg tggaggtctc
      241 tcattcccag cagcagaagt tcgaacggct gatgagtggt ctgccttgtg cattgttggg
      301 agagactgtg gaagatgcca gtttgaaggt ggtcggccgc actggagaag ttgccattga
      361 ggtagctttg gatgatatcg aacgcagttg gaagcgctcc ttgtggacgt cttgagcggg
      421 tcaaggtaag tcaggcggat ttccgctcaa cggagcatcc gttcgattga gcatcgttcg
      481 cgcgccagtt ggcgctgaag gaagaatatg gctcgagtga gacagaagaa agaaattcgt
      541 gccgctgtgc ttcgtgcggc gggaaccaat tgtgactatg aaaccggata tgccctgacg
      601 ctggcgggtg cccggcctgt tctcgttcat gtcagagaac ttctggacgg gatcaagaaa
      661 ctcaacgact atcatctgct gatcattccg gggggatttt cctacggaga cgatttgggt
      721 gctggcaaac tcctggccaa tgaagttcgg tatctcctgg gtgaggagat gactcgtttt
      781 gtcaaagcag ggaaacctgt tttgggtatc tgcaacggat tccaagtgct tgtgaaggca
      841 ggttttcttc ccgaatttca gcccggcgaa tggtctcagg aaatgtcact ggtggcaaac
      901 gattcggctc gcttcgaatg ccgatggact tttctgaaac ccaatccaaa atcgccatgc
      961 gtcttcacgc aaggcatcac tgaacccata ccggttccca tcgctcacgg tgaagggaaa
     1021 gttgtcttcc gagatgccct cattcaggag caggtcgtca acaacaatct ggttgctttc
     1081 cagtacgtgg actcggaagg caatgccggt ccctatccag tcaaccccaa tggatctgcc
     1141 tgggacattg cggggatctg tgatccgaca ggaagaattc tcggtatgat gccccatccc
     1201 gaacgacacg ttcatcaatg gcagcatccc tggtggactc gacggcgaca caacgaagca
     1261 agaggggctg gactccgcat ctttgagaac gcagttcaac tgtgcaaaga cagtctctga
     1321 caccggctgc gtcagactcc acaaacaata tcccgtacag gtatagacat tccttcctct
     1381 tttggttaca ttgcgtcttc agtgagttca tgaaatggag agtcgtgttc gattatgggc
     1441 gaatatgtac gcaagcctga ttttcagagg ctgagaaaga ctctgcttct gcagggcgag
     1501 gcagactgtg ttccacttgc ggaactgggt attgacaatt ccatgaagga atttgtcctg
     1561 gggcgtcctg tggagacgct tcaggatgag gtggatttct cgaaagtggc gggctacgac
     1621 tatgtcaaat tggcgccagg cattgatctg aacccagcga acattcagcc caaagaaggc
     1681 agtcgtgccg cagatgctac acagcatgat tccgcaaggg tctttgcgaa tgaaggacaa
     1741 gggatcatc
//
LOCUS       DAOQUC010000961         1748 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_90211_length_1748_cov_2.889545, whole genome shotgun sequence.
ACCESSION   DAOQUC010000961 DAOQUC010000000
VERSION     DAOQUC010000961.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1748)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1748)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1748
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90211_length_1748_cov_2.889545"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            97..939
                     /locus_tag="PKH07_14500"
     CDS             97..939
                     /locus_tag="PKH07_14500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007294092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HNT36193.1"
                     /translation="MAEFFTAILDPDFAFLRNALVVGFLASISFGIVGTYVVARRISS
                     MAGAISHCVLGGIGAALFLREWMGWAWLDPVHGAIVAGLVSAVAIGLVSLHAKQREDT
                     VIGALWAVGMAVGILFLSKTRAYVDPMSYLFGNILMISREDLWIVVVLDLVVICVSVF
                     FYNRFLALCFDEEFAMLRGVKVKAYFLLLLCLTALAIVLLVRVVGIVLVIALLTLPAA
                     VAGNLAKRLWQMMLLAIVLCMAFVVFGLALSYTWNLPAGATIIVVAGLVYLLFVVFQR
                     FVRS"
     gene            1337..>1748
                     /locus_tag="PKH07_14505"
     CDS             1337..>1748
                     /locus_tag="PKH07_14505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36194.1"
                     /translation="MISKSATESAEQPFFTSRSITVSPQDLLTWREWPERAAQAGLNT
                     LCIAPPSVEFVRSDDGQAILEQCKTIGLEVEYNMHAMGILLPRELFDKEPDLFRMDTD
                     GIRQRDGNLCVHSEKALEIVSQNVVELAKVFRPTT"
BASE COUNT          339 a          454 c          516 g          439 t
ORIGIN      
        1 cgagtgaact gaatggaact ctgatccgcg atctctatgg tggggatttt ctcatggttc
       61 gccatgacca tcgctgcagt gaagaaggac accaacatgg ctgagttctt cactgcgatt
      121 ctcgacccgg actttgcctt cctgcgcaat gcgcttgttg tcggctttct ggcgagcatt
      181 tcctttggaa tcgttggaac ctatgttgtg gcgagaagga tcagctccat ggcgggcgcg
      241 atctcccact gcgttctggg gggaatcggg gctgcgcttt ttctgcgtga atggatggga
      301 tgggcgtggc tggatccggt tcatggagcc atcgtggcag ggcttgtttc tgccgttgcg
      361 attggtctgg tgagtctgca cgccaaacag cgtgaagaca cagtcattgg tgctctttgg
      421 gcagttggca tggcggtggg catactgttt ctctccaaga cacgcgccta cgttgacccc
      481 atgagttatc tgtttggcaa catcctgatg atctctagag aagatctatg gattgtggtg
      541 gtcttggatc ttgtcgtcat ctgtgtcagt gtcttcttct acaaccgatt tctcgcgctg
      601 tgttttgacg aggaatttgc catgttgcgg ggagtgaagg taaaggccta cttcctgctt
      661 ctgttatgtc tgactgccct tgccatcgtc ctgctggttc gtgtggtggg cattgtgctg
      721 gtcattgcct tgttgacact gcctgccgcg gtcgccggaa acttggccaa gaggctttgg
      781 cagatgatgc ttctcgcgat cgtgttgtgc atggcgttcg tggtttttgg tctggcgctg
      841 agttacacgt ggaacctgcc cgcgggagca acgatcatcg ttgtcgccgg tttggtctat
      901 ctcctctttg ttgtgtttca acgttttgtc aggtcttagc gagcagccac gtttcccgaa
      961 accagagatc ctgggttgag ggcaagccag gctactgaat gtcatgctga gcaaagcgac
     1021 gctctgggag cgaagagagg aatctctctg aaacggagcg tgagccaaga gagattcttc
     1081 gccacgttcg cagagcctca ctccgttccg aagggctggg ggcgcgggcg tctcgcccgc
     1141 gaagatgggg cggaagcatt cagcccccga atgaaagaag tgtcgggcag gacgtcccac
     1201 cgaagccggg gatcgcttgt cagcaacacc tgcgtcgagt atactcactc tcatgaaata
     1261 tgatgctctc gacgtagcaa tgtgctctcg acgcagtttt atcgctctcg tcgggggctc
     1321 attcctggga ggaactatga tttcgaaaag cgcaaccgaa tccgctgagc agcctttctt
     1381 tacaagccga agcatcacgg tatcgccgca ggatctgctg acttggcgcg aatggcctga
     1441 acgagctgcc caggcaggct taaacacgct gtgcatcgca ccacccagcg tagagtttgt
     1501 ccgctcagac gacggtcaag ccatcctcga acagtgcaag accatcggac tggaagtgga
     1561 atacaatatg catgccatgg gcatcctact accgcgcgag ctgttcgaca aggagcccga
     1621 cctgttccgc atggacacgg atggcatccg acaacgcgac ggcaatctct gcgtgcattc
     1681 ggagaaagca ctggagatcg tttcgcagaa cgtcgtcgaa cttgccaagg tcttccgccc
     1741 cacgactg
//
LOCUS       DAOQUC010000962         1748 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_90237_length_1748_cov_1.938571, whole genome shotgun sequence.
ACCESSION   DAOQUC010000962 DAOQUC010000000
VERSION     DAOQUC010000962.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1748)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1748)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1748
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90237_length_1748_cov_1.938571"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(246..1520)
                     /locus_tag="PKH07_14510"
     CDS             complement(246..1520)
                     /locus_tag="PKH07_14510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HNT36195.1"
                     /translation="MKSTQNAKILQLTPETLVIGIDIGKATHYARAFDYRGIELTKVL
                     RFSNTREGFESFDRWKHDTMQKYGKTEVRVGFEPTGHYWFALGDHLQKTDCKLSIVNP
                     FHVKCSRELDDNSQTKTDRKDPETIAKLVKDGRYREVYIPQDVYMELREAVSGHEAIR
                     NQQGALSNQIIRWLDIRFPEFTEVFANWRGIAATEVLKHCPTPSMILALGAEGVMKIW
                     RKRLMKPSSKKAEKLLLAAERSIGKRDGEKAATQSLKCLLAQHELLEVQCREQETLMQ
                     ELLLQVPNAYKLLDIKGIGNVLAAYIVSELGDIHRFQDPRQIQKMAGLALRENSSGKH
                     KGKTTISKRGRRLLREALFRAVIAMLATNDEFRALHQRNLTREKNPLTKMQSVIALCG
                     KLIRVVFGILTKGFEYDPGKLMLDATRSMIAA"
BASE COUNT          336 a          506 c          395 g          511 t
ORIGIN      
        1 tctccacagc gtggtacgtc aatcatgctc cttagcaggg tcttcgccct aaggtgtaat
       61 tcaacttgac tgccccggct cttctttcaa tgtgcgtttc ctgcgtcggt cagagattga
      121 gaatttcccc gtacaaggct cttccacagc ggcttttgtg ggatcccctt catacttcgt
      181 cgtgccagtg gttacaggtc ggtcggtcag atgatgtcgg tcggtgtctt tatcgccttc
      241 agtgcttacg ctgctatcat tgaacgggtg gcatcgagca tcagcttgcc agggtcgtat
      301 tcaaaccctt ttgtcagtat cccgaacacc actcgtatca acttgccgca caacgcaatc
      361 actgactgca tcttggttaa cgggtttttc tcccttgtca ggttcctttg gtgcaacgcc
      421 ctgaattcat cgttggtcgc gagcatggcg atgactgccc gaaacaacgc ttctctcaac
      481 aaccgccgtc cgcgcttgct gattgtggtt tttcctttat gcttcccgga gctgttctcc
      541 ctcagcgcca ggccggccat cttctggatt tgacgcggat cctggaacct gtggatatct
      601 ccgagttcgc tcacaatgta tgccgccagc acgttgccaa tccctttgat gtccagcaac
      661 ttataagcat ttggtacctg taacagcaac tcctgcatga gggtctcctg ttcccggcat
      721 tgcacttcca gcagttcatg ttgtgcaagc aggcatttga gtgattgcgt tgccgctttc
      781 tctccatctc gcttgccaat ggaacgctct gccgccagca ggagcttctc tgccttcttc
      841 gaactcggct tcataagccg ctttcgccat atcttcatga ccccctcggc gccaagcgcc
      901 agaatcatcg atggggtggg acaatgcttg agcacctctg ttgccgcgat cccccgccag
      961 ttggcaaaca cctccgtaaa ctcagggaaa cgaatgtcaa gccaacggat aatctggtta
     1021 ctcagcgccc cctgttggtt tcggatggcc tcatgccctg aaaccgcttc gcgcagctcc
     1081 atgtaaacat cttggggaat gtagacttcc ctgtagcggc catcctttac cagttttgca
     1141 atcgtctcag ggtccttcct gtcggtcttc gtctggctgt tgtcatccag ttcccgtgag
     1201 catttcacgt gaaatggatt gacgatggac aacttgcaat ccgtcttctg cagatgatct
     1261 cccaacgcga accagtaatg tccggtaggc tcgaagccta cccgaacctc ggttttccca
     1321 tacttctgca tggtgtcgtg cttccacctg tcgaaggact cgaatccttc cctggtgttg
     1381 ctgaaccgca gaacctttgt cagttcaatc cctctgtagt cgaaggctct tgcgtaatgg
     1441 gtggccttcc caatgtcgat tccaatgacc aaagtttccg gtgtcagttg caaaatcttc
     1501 gcgttctgtg tactcttcat cttgagggcc tcctcgtcac ttgatttgat gccgttttgc
     1561 ttgttgcttt ggcacaattc tagtttacga ggtggcttct cgtttttcaa agtccgctag
     1621 agttcattac aggaatgctc caatattgtt tcacatatca gtctcgagat tgcaatttat
     1681 ctgagaggat ctgttctcat acaaccatct catcatccga atcatcatcg acttccttac
     1741 cactcaca
//
LOCUS       DAOQUC010000963         1747 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_90279_length_1747_cov_3.093972, whole genome shotgun sequence.
ACCESSION   DAOQUC010000963 DAOQUC010000000
VERSION     DAOQUC010000963.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1747)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1747)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1747
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90279_length_1747_cov_3.093972"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1040)
                     /locus_tag="PKH07_14515"
     CDS             complement(<1..1040)
                     /locus_tag="PKH07_14515"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNT36196.1"
                     /translation="MLDKRTRFHYGWVMLTMAVLVTLSSIGLARFGYTMILPAMQRGL
                     DLSNTQTGALATANFIGYLVLALVGGFLASYYGPKRVIALSLGLVGVSMILTGFSKSF
                     AQGLLWRAMAGIGSGASNVPMMGLLAAWFAPRRRGLATGIAVSGSSLGLIVTGPLVPL
                     ILNASELDGWRWSWFILGGFVLLVAVVARFFLQDRPSDRGLLPVGAAPAEPAASVQGA
                     PLGMRSLALVHRSPIVWKLSLIYTAFGFSYIVYATFFAKYLQSEVGLTRQGAGRLWLT
                     AGWISLFCGLLWGWISDLIGRRSALILVNLIHATAYLIFALWTEPLGRYCSVVLFGLT
                     AWSIPAIMAAACG"
     gene            1393..>1747
                     /locus_tag="PKH07_14520"
     CDS             1393..>1747
                     /locus_tag="PKH07_14520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018226495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HNT36197.1"
                     /translation="MNIVMMGVQGSGKGTQAKMLSAHLGLPHISVGDIFRANIAQGTE
                     LGKRAQTYTDKGLLVPDEVVMDMMADRLKQADVAGGLLLDGFPRNAVQMACLEFVRPP
                     EHVIYLDLDDVTAILR"
BASE COUNT          449 a          507 c          471 g          320 t
ORIGIN      
        1 ccgcaagccg ccgccatgat ggcgggaatg ctccaggcgg tcagtccaaa aagaactaca
       61 gagcagtagc gtccgagggg ctctgtccaa agagcgaaga tgagataggc cgtcgcatga
      121 ataaggttga ctagaatcag cgcagatctc ctgccaatga gatccgaaat ccatccccag
      181 agcagaccac agaacagact gatccagccg gcagtcagcc agagccttcc cgctccttgg
      241 cgagtcagcc caacttccga ttgcaggtac ttggcaaaaa aggtcgcgta cacaatgtaa
      301 gagaaaccaa acgccgtata gatgagcgag agtttccaca cgatgggaga acgatgcacc
      361 aaggccagag agcgcatgcc caggggcgct ccctgcacgc ttgcagccgg ctcggctgga
      421 gcagccccca caggcagaag cccgcggtct gacggtcggt cttgaagaaa aaatcgagcc
      481 acaacagcaa ccagcagcac aaaccctccc agaatgaacc aactccatcg ccagccgtcc
      541 agttccgagg cattcagaat cagtggcacc agcggtccgg tcacaatcag ccccagactt
      601 gatccgctga cggcaatccc tgttgccaag ccccggcgtc tgggggcaaa ccaagcggcc
      661 agaagcccca tcattggaac gttgcttgcg ccgcttccga tgccggccat ggcgcgccaa
      721 agcaaccctt gagcgaatga cttcgagaaa ccagtcagga tcatgctgac tccaacgaga
      781 cccagcgaca gggcgatcac gcgtttggga ccgtagtagg aagcaaggaa tccccccacc
      841 agagcaagaa ccagatagcc gatgaagttc gccgttgcca aggctcccgt ctgcgtgtta
      901 ctgagatcaa gcccccgctg cattgcaggc aggatcatcg tatagccaaa tcgcgccaac
      961 ccgatggaac tcaacgtgac cagcaccgcc atggtgagca tgacccatcc gtaatggaac
     1021 cgagtcctct tgtcgagcac ctttcacctc accgctatta caaagtgaaa cgactgtaca
     1081 ccaaattgct caatgttgga aaatgaaaga ggagagagct aatcgtgtgc cccaacagat
     1141 tcaggcactt ctccaccagg ggatcagatt gtacctgtgc ttgaccacaa ccatctgatt
     1201 ttcctcttga accagattgc ccagaccgat agtatgccaa cgggtctgca cctgtgcaag
     1261 gaacgttggc tggatccact cgccggcggt tgtcttcttg aggagtgtag actcagagac
     1321 agggatgctg tcagcaataa gcagccgctg acagtgtaat cgtgtgttga caccaaccaa
     1381 ggagttgtag cgatgaacat tgtcatgatg ggcgttcagg gatcgggcaa gggaacacaa
     1441 gccaagatgc tctccgcgca tctcggactg cctcacattt ccgtcgggga tatctttcgc
     1501 gccaacattg cgcaaggaac cgaactgggc aagcgagcgc agacctacac cgacaaggga
     1561 ctgctggttc ccgacgaagt agtcatggat atgatggcgg atcgtctgaa acaagcagat
     1621 gtcgcaggtg gccttcttct tgacggcttt ccgcgaaatg cggttcagat ggcctgcctg
     1681 gagttcgttc gacctcccga gcatgtgatc tatctcgatc tggatgatgt gacggcgatt
     1741 cttcggc
//
LOCUS       DAOQUC010000964         1745 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_90497_length_1745_cov_2.609467, whole genome shotgun sequence.
ACCESSION   DAOQUC010000964 DAOQUC010000000
VERSION     DAOQUC010000964.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1745
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90497_length_1745_cov_2.609467"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..601)
                     /locus_tag="PKH07_14525"
     CDS             complement(<1..601)
                     /locus_tag="PKH07_14525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36198.1"
                     /translation="MDIEKIIRTLSRNQTPFLGEELPDDLLQGFVEGTLTEEERRRCV
                     DLLSRHPEELAVLAVLAQEQDVSGEAAILSAEEVASLWKSIPVCTIHCGVAREGFKLL
                     ESERSGFHGTGELLSPPKCISGKLGLPLVWPADRAEFDVSFGDSVVSVTFGLVSGSIE
                     GTVLVQRPPEPELKFEFDVYENETFLSSEQVIPGRSYRFE"
     gene            complement(671..1267)
                     /locus_tag="PKH07_14530"
     CDS             complement(671..1267)
                     /locus_tag="PKH07_14530"
                     /inference="COORDINATES: protein motif:HMM:NF016430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNT36199.1"
                     /translation="MEETGFIQAFVSDPSGHWMEFTQRYGGVIKGQVFHLGFRGQDAE
                     DALQEVFYELFKRDLARIRQWNPQRGAFMTFLRVVVSRILVDLHRSPKFRRDAQLDEN
                     SPELSCPSEGNKEADARECAAEEEIFGLIQQHLRQLVQSGSADAVDMLIFHCRYFELP
                     GAVTARLTGLSENAVNLRFHRLKQEIQKFLHLKGLQII"
     gene            complement(1267..>1745)
                     /locus_tag="PKH07_14535"
     CDS             complement(1267..>1745)
                     /locus_tag="PKH07_14535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36200.1"
                     /translation="KSQQRLRSDYGGNDIDTYLPDTLGCGIAYLPNKRWRAVLDVDHI
                     WWSKFEPNPNDAFVKDDVWRYHTGLEWYAGRFRGTGIFLRGGYFYEQPNSYRYTASPG
                     DPKYNRLLEELSSERDAVNHYTFGFGLARSQYQLDFGMDYGDDTTVDFITSVVVYF"
BASE COUNT          443 a          494 c          405 g          403 t
ORIGIN      
        1 cctcaaaacg ataggaccgg ccgggaatga cctgctccga ggacagaaat gtctcgttct
       61 catagacatc aaattcgaac ttcagctcgg gttctggggg acgttgaacc aaaactgtgc
      121 cttcgataga ccccgacacc aaaccgaatg tgaccgaaac gacactgtcc ccaaaggaaa
      181 cgtcgaactc ggcacggtct gcaggccaga ccaggggaag cccgagtttt cctgagatgc
      241 actttggagg agaaaggagc tcacccgtcc catgaaaacc agatctttca gattcgagaa
      301 gtttgaaccc ctcccgcgca acaccacaat ggatcgtgca gacgggaatg ctcttccaga
      361 ggcttgccac ctcttccgca ctcaaaatgg ctgcctcacc cgaaacgtct tgctcttgag
      421 cgagaacggc gaggacagca agttcctcag ggtgcctcga aagcagatca acgcaacgtc
      481 tgcgttcctc ttccgtcaaa gtcccttcaa caaatccttg gagcagatcg tctggcaact
      541 cctctcccag aaaaggcgtt tggtttcggc tcagtgtcct gatgatcttc tcaatgtcca
      601 tatctgccac cgttctctgt ccctttatgc cctttcatta aaacaatacg agactgcgga
      661 tacctttctt tcaaataatc tgaagccctt tgagatgaag gaatttctgt atctcctgct
      721 tcaaacgatg gaaacggaga ttcactgcat tctcagacag tcctgtcaac cgtgccgtca
      781 cagctcctgg gagttcaaag taacggcagt gaaatatcag catgtccacg gcatcggcac
      841 tgcccgactg cacaagctgc ctcaagtgtt gttggatgag gccaaagatt tcttcctcgg
      901 ctgcgcattc tctggcatcc gcttctttgt tcccctcaga agggcaactc aattccgggc
      961 tgttctcgtc cagttgagca tccctgcgaa acttcggact gcggtgcaaa tccaccagga
     1021 ttcgagaaac gacaaccctg agaaacgtca tgaatgcgcc cctctgtgga ttccattgac
     1081 ggatgcgagc cagatcccgc ttgaatagct catagaaaac ttcctgaagt gcatcttcag
     1141 cgtcctgtcc tctgaatcca aggtggaaca cttgcccctt gatgacgcct ccgtatcttt
     1201 gggtgaactc catccaatgc ccggacggat cactgacaaa cgcttgtatg aaaccagttt
     1261 cttccatcag aagtacacaa ccaccgaagt gataaaatca actgtcgtgt cgtcaccata
     1321 gtccatgccg aaatccagtt ggtactgcga acgagccagg ccaaacccaa atgtgtaatg
     1381 gttcaccgcg tcccgctcgg aagagagttc ctccaaaagt cggttgtact ttgggtcacc
     1441 aggcgaggcc gtatagcggt aagagttcgg ctgttcgtaa aagtaccctc ctctgagaaa
     1501 aatgccggtg ccgcgaaagc gaccggcata ccattcaagg ccggtatgat aacgccatac
     1561 atcatctttg acaaaggcgt cattgggatt gggttcaaat ttcgaccacc agatatggtc
     1621 aacgtccagc acagcgcgcc atcttttgtt gggaagatag gcaataccgc aaccgagtgt
     1681 gtcaggaaga tacgtatcaa tatcgtttcc cccatagtca ctgcgcaacc gctgctgact
     1741 cttgt
//
LOCUS       DAOQUC010000965         1744 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_90557_length_1744_cov_3.087626, whole genome shotgun sequence.
ACCESSION   DAOQUC010000965 DAOQUC010000000
VERSION     DAOQUC010000965.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1744)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1744)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1744
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90557_length_1744_cov_3.087626"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..247
                     /locus_tag="PKH07_14540"
     CDS             <1..247
                     /locus_tag="PKH07_14540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HNT36201.1"
                     /translation="PDLFRKAAGFEKTDPTTGTRFTWIQNESLEDLERFDRVQQIKQE
                     YEARIESQRNMNTAQSTLLDVFSEDAASESSCLICHL"
     gene            253..1131
                     /locus_tag="PKH07_14545"
     CDS             253..1131
                     /locus_tag="PKH07_14545"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HNT36202.1"
                     /translation="MPERATSVEREIYLDYNSTTPCAKEVVDVMLPYLSQQYGNPSSP
                     HLMGKRAYAACSDAREQVSELLGCSSAEIVFTSSATESNNLVLKGVMAKAQRRKIVTT
                     AVEHKSVLEPCRVIRDQGIEVVVVPANEEGVVDLERAKDAIDEGTALVSVQGANNETG
                     VIQPIEHLMKIAHSKGALFHCDAAQLLGKVPLPRSFVEVDYATFSAHKIYGPKGLGIL
                     YIRKGIAHRFLQPILHGGSQEGGLRAGTLNVPGVIGAAEACRIVRERVVRDVREITAL
                     RDRFERLIIEALPHAG"
     gene            1186..1416
                     /locus_tag="PKH07_14550"
     CDS             1186..1416
                     /locus_tag="PKH07_14550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36203.1"
                     /translation="MSEQIHYKEIPDGFEYGAAKVSRLFSNPKNGWTITEISTPKTSI
                     QVYVTKTGNLRVFGDGGIELKLTQKHSREVKR"
BASE COUNT          528 a          337 c          482 g          397 t
ORIGIN      
        1 cccggatctt tttcggaagg cggcaggatt tgagaagacg gatccgacaa cagggacacg
       61 gtttacttgg atacaaaacg aaagtcttga ggatttggag cgatttgatc gtgttcagca
      121 gatcaaacag gaatacgagg cacgcatcga gagtcagcga aatatgaaca cggcacaatc
      181 cactttgctt gatgtgttct ctgaagatgc cgcttcagag agcagctgcc tgatatgtca
      241 cctgtgatgg ggatgccgga aagggcgact tcagtggaac gcgagatata cctggattac
      301 aattcaacga ctccttgtgc aaaggaagtg gttgacgtca tgttgccgta cctgagtcag
      361 caatacggaa atccatctag cccacatctt atgggcaaac gggcatatgc agcatgcagt
      421 gacgcaaggg agcaggtgag tgagttgctt ggctgttcca gtgccgagat tgttttcacg
      481 agttcggcaa ccgaaagcaa caatcttgtc ctgaaaggag tcatggcaaa agctcaacgg
      541 agaaagattg ttacaacagc agttgaacac aagtcggtct tggagccatg tcgagttatc
      601 agagatcaag ggatagaagt tgttgtggtt cctgcaaatg aagaaggtgt tgtcgatctt
      661 gaacgtgcca aggatgcgat tgacgaaggg acggcgttgg tctccgttca gggagctaac
      721 aacgagacag gagtcataca acctatcgag catcttatga agattgcgca cagcaaaggc
      781 gcgctctttc actgcgatgc ggcgcagttg ctaggcaagg ttccgctgcc aagatcattc
      841 gtggaggtag actatgctac gtttagcgct cacaaaatct acggtccgaa ggggcttggc
      901 atcctctata taaggaaagg tattgctcat aggtttcttc aaccaattct ccacggcggg
      961 tctcaggaag gcggactgcg tgctggaaca ctgaatgttc ctggggtaat tggtgcagca
     1021 gaagcctgtc ggatagtcag ggaaagagtg gtaagggatg tgcgtgagat aacagccctc
     1081 cgagacagat ttgaaaggct gattatagaa gcgttgccac atgcgggctg atactttgag
     1141 cggttctgat ggtggctttc gtaatgctga tggagaggtg aagagatgag cgaacaaata
     1201 cattacaaag aaatccctga tggttttgaa tacggagcag caaaagtttc tcgcttgttt
     1261 tcaaatccaa aaaatggctg gacaatcacg gaaattagta ccccaaaaac aagcatacaa
     1321 gtttacgtaa ctaaaactgg gaatcttcgc gtgtttggag atgggggcat agagttgaag
     1381 ctaacacaaa agcacagtag agaggtgaag agatgacaaa ctggcgaaac ttgacagaca
     1441 cacaacagcg atttatcctg cgaaaatatc taatggattc ctacaaaaat gaagtccaga
     1501 aactcacagc gcgggagcgc agagagtatg caatggagat ggggctgatc gacagatggg
     1561 gaaggttgta tttagatatc gcagaaatga aaacaaacca taagaggtga agatatgagt
     1621 aacgaaagtt tctgtatcgt gaatccaagc ttcctttgtg agatgaaaaa taaccgaact
     1681 gtcaatgaat gcaagcatta caaaaaggct cgtaagcatc ttggatgtga ttaccaaatg
     1741 gaag
//
LOCUS       DAOQUC010000966         1744 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_90575_length_1744_cov_2.668443, whole genome shotgun sequence.
ACCESSION   DAOQUC010000966 DAOQUC010000000
VERSION     DAOQUC010000966.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1744)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1744)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1744
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90575_length_1744_cov_2.668443"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="PKH07_14555"
     CDS             complement(<1..222)
                     /locus_tag="PKH07_14555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HNT36204.1"
                     /translation="MWNPKVECMPRKDVQALQLERLRHTVGRVYQNVPAYREKFDKAG
                     VKPQDIKTLEDLQRLPFTAKDDLRQNYPFG"
     gene            complement(222..716)
                     /locus_tag="PKH07_14560"
     CDS             complement(222..716)
                     /locus_tag="PKH07_14560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013505311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HNT36205.1"
                     /translation="MSSQESFQVIFAGVGGQGVLLASEITALVAFEEGLDVKKSEVHG
                     MSQRGGSVVSHVRMGERVFSPLISKGQGDALVAFEASESCRYEAFLKPDAVRIDPSSI
                     SMEALPHPRTANIFIVGCLARLLPFQQRTWEKVISEMVHPNTLEMNLEAFRRGLKVSP
                     REAQ"
     gene            complement(716..>1744)
                     /locus_tag="PKH07_14565"
     CDS             complement(716..>1744)
                     /locus_tag="PKH07_14565"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNT36206.1"
                     /translation="CGASYHYAREAAPEATFLKLGFSYPLPESLIRDFFSRVKKVAVV
                     EELEPFLEEQLLQMGLSLTPKPVAFRLGEYDPDRVRELLGRPVAEVVGAAATPGRPPV
                     MCAGCPHRPVFTVLKKMKMIVTGDIGCYTLGTLPPLSALDTCVCMGAGIGHAFGMTRV
                     LPEEQRRKVVAVIGDSTFIHSGITPLIDMVYNKGCSTVIILDNRTTAMTGRQEHPGTG
                     RTLKGDPAKQLDLFKLCQSVGVEDVRMVDPYDLAAVQEAITHAVESDQPSVIITNRPC
                     VLIRSNRTEALWIDEEKCRSCGLCAKTGCPAILDREGKFMIDSSKCWACGLCRQVCPF
                     EAISEREN"
BASE COUNT          432 a          505 c          426 g          381 t
ORIGIN      
        1 gccgaaggga tagttctgtc tcagatcatc ctttgcagtg aaaggcagtc gctgcaagtc
       61 ctcaagagtc ttgatgtctt ggggcttcac accagccttg tcaaacttct ctctgtaagc
      121 gggcacattc tgatagactc gacccacggt gtgcctcaac cgttccagtt gcagagcctg
      181 cacgtcctta cggggcatgc attccacttt tgggttccac attattgagc ctccctcgga
      241 gataccttca gacctcgccg aaatgcttca aggttcatct ccagagtgtt cggatgtacc
      301 atctcgctga tgaccttctc ccatgtccgc tgctgaaagg gaagaagtcg agccaggcag
      361 ccgacgatga aaatgttcgc tgttcgtgga tgcggaagtg cttccatcga aatcgaactt
      421 ggatcaattc gaaccgcgtc aggtttgaga aaagcttcgt aacggcagga ctcggatgcc
      481 tcaaaggcaa cgagcgcatc tccctggcct ttcgagatga gcggggagaa cacccgttca
      541 cccatgcgaa cgtggctgac aacactgccc cctcgctggg acatcccatg cacttcactc
      601 ttcttgacgt ccagaccctc ttcaaaggcg accaaggcag taatctcact ggcgagcaag
      661 actccctgcc ccccaacacc ggcaaaaatg acttgaaagg actcctgcga tgacatcaat
      721 tctccctctc cgaaatggct tcaaatggac agacctgccg acaaagtccg caagcccaac
      781 attttgaaga gtctatcatg aacttgcctt ccctgtccag gatggcagga caacctgttt
      841 tggcacagag cccacagctg cgacactttt cctcatcaat ccacagggct tcagtgcggt
      901 tggatcgaat gagaacacac ggacggtttg tgatgatgac tgagggctga tctgactcca
      961 cggcatgagt gatggcttct tgcactgccg caagatcgta gggatccacc atcctgacat
     1021 cttccacccc cacgctttga cacaacttga acaaatccag ctgctttgca ggatcgcctt
     1081 tgagtgtgcg tccggtgcct ggatgttcct gacgaccagt catagccgtg gtgcgattgt
     1141 caagaatgat gacggtcgaa catcctttgt tgtagaccat atcaatcaga ggcgtgattc
     1201 cggagtggat aaaggtcgag tctccaatga ccgcaaccac cttcctgcgc tgttcttcag
     1261 gcagcacacg agtcatccca aacgcatggc cgatccccgc tcccatacaa acacatgtat
     1321 ccagagcaga caaaggcggc agagtcccga gggtgtaaca accgatatcg cctgtgacaa
     1381 tcatcttcat cttcttcagg acggtgaata cgggacgatg aggacaccca gcacacatga
     1441 caggcggacg ccccggagtt gccgcggccc caacgacttc ggccaccggt cgaccgagaa
     1501 gttcacgaac cctgtccgga tcatattctc caagccgaaa cgcaaccggt ttgggcgtga
     1561 gggatagacc catctgcaaa agttgttctt caaggaaagg ttcgagttcc tcaacgacag
     1621 caaccttctt gacacgggag aaaaaatccc taatcaaact ctccggtagt ggataggaaa
     1681 aaccaagttt caggaacgtt gcttccggag ccgcttcacg cgcatagtga tacgaagcac
     1741 caca
//
LOCUS       DAOQUC010000967         1740 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_90867_length_1740_cov_3.284866, whole genome shotgun sequence.
ACCESSION   DAOQUC010000967 DAOQUC010000000
VERSION     DAOQUC010000967.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1740
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90867_length_1740_cov_3.284866"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(205..900)
                     /locus_tag="PKH07_14570"
     CDS             complement(205..900)
                     /locus_tag="PKH07_14570"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNT36207.1"
                     /translation="MNHPWQYDETIQVGTDYSDQSEVAAYDQRMQKLRDVNAETQAIK
                     AAFSLSDTSVVWEIGTGTGECALNLATSVEHVYATDVSPAMLEYAKHKAEQRCIGNVT
                     FALGGFLSGFHPPHPVDGVVTQLALHHLPDFWKSKALAGIADRLRPSGRLYLRDVVFP
                     SAVDDHDVFFLEVIDGIRRSAGDELVQQTIRHIKKEFSTLDWILEGMIVRSGLRIVEK
                     DAQGFFSVYVCEK"
     gene            1378..1737
                     /locus_tag="PKH07_14575"
     CDS             1378..1737
                     /locus_tag="PKH07_14575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020589448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HNT36208.1"
                     /translation="MNVAILGASNKPDRYSYKAFKSLQANGHTPLPVHPVLAEIEGVP
                     VHRSLSAVPVTIETITVYLSAANSDAIADEILNCGARRVIFNPGAENPPLETQLQARG
                     IEVLKACTLVLLSTGAF"
BASE COUNT          378 a          479 c          460 g          423 t
ORIGIN      
        1 cgagatctgt tcatgcctta tgtggttcta tagacggtgg ggtaacagat gcgttctctt
       61 tcccatgtgg cagtcttcaa ctcgttgagg actgcttctc gatgtgacac aggcattcgt
      121 gcctgtgagg atgtagggtg gatgtagggt ggagcgagtc tgcgagttcc accctcgtgg
      181 ctgtcttatg cttcgttttt ggtttcattt ctcgcaaaca tagactgaga agaagccttg
      241 ggcgtctttc tcgacgattc tcaggccact tcgaacaatc atgccctcca gtatccagtc
      301 cagggtcgag aactccttct tgatatgccg tatcgtctgt tgcaccagct cgtcaccggc
      361 actgcgacga atcccgtcaa tcacttcgag aaagaacaca tcatggtcat caactgccga
      421 gggaaagaca acatcacgaa gatacagcct tccggaggga cgcaggcggt ctgcgattcc
      481 agcaagggct ttcgatttcc aaaagtcagg caggtggtgt agagccaatt gggtgactac
      541 gccatctaca ggatgaggcg ggtgaaagcc ggacaggaat cctcccaacg cgaatgtcac
      601 attgccaatg cagcgttgct ctgctttgtg cttggcatac tccagcatgg ccggagaaac
      661 atcggtcgca tagacatgtt ccacgctggt ggcaaggttc agggcgcact cccctgtgcc
      721 cgtgccaatc tcccagacga ccgaagtatc cgacaatgaa aacgccgcct tgatggcctg
      781 cgtctcagca tttacatctc gcaatttctg catccgctga tcgtaagcgg cgacttcgga
      841 ctgatcactg tagtcggtgc ccacctgaat cgtttcgtcg tattgccatg gatggttcat
      901 gttcgtctac tcggaggacg atatcttaac gagttccatc tcgtcaagtg ccgggtgccc
      961 tctctttcct gtgtagcagc cttcaactcg ttgaactctg cttctcgatg tgacacaggc
     1021 attttgcctt tgatcttctg ttctgagaca cagacaggaa tgtctgttcc acactgccct
     1081 gggatcgccg agcgcctgct cggcaactcc ttctcctgtg ggagaaggtt tgggatgagg
     1141 gcatcggagc gggagcatct tgctcccgac aaaatccgtg tggggcgaga cgctccagct
     1201 cctgaaggat ttgaccgtgt gtctcgaata tgagagattt cttatcatga taagtttcgc
     1261 agtgcaggtt gtggctgcca gcaaccgcaa aggtgtcagc cgtacagaaa gatcgaacct
     1321 cgtgtcactg cggatgcctg cgggaataaa gcgcaatgca tcgaggagga gtctgccatg
     1381 aacgttgcca ttctcggagc tagcaacaaa cccgaccgat acagttacaa agccttcaag
     1441 agtcttcagg cgaacggaca cactccgctc cccgttcatc ccgttcttgc agaaatcgaa
     1501 ggtgtgccgg ttcaccgtag tctctcggca gtccccgtga ccatcgagac gatcacggtc
     1561 tacctttctg cggcaaactc cgatgcgatt gccgatgaga ttctcaactg tggtgcgcgt
     1621 cgagtcatct tcaacccagg tgcggagaac ccccctctcg aaacacaact gcaagccagg
     1681 ggcatcgaag tcctcaaagc ctgcaccttg gttcttctga gcaccggagc gttctgaccg
//
LOCUS       DAOQUC010000968         1739 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_90965_length_1739_cov_2.558789, whole genome shotgun sequence.
ACCESSION   DAOQUC010000968 DAOQUC010000000
VERSION     DAOQUC010000968.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1739
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90965_length_1739_cov_2.558789"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..682
                     /locus_tag="PKH07_14580"
     CDS             <1..682
                     /locus_tag="PKH07_14580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36209.1"
                     /translation="AYVGDPEPERRGGVIAISPDGSASELVRTPWMPRGATLSRRFLY
                     TVEALPYVLPDEPAGDGSYVGVSAEIRFCRHDCSRLYETADVIVLRGESETCRWFAEL
                     AANRVGNQVLILPSRRCEEIVFMVSTGTGEILQVTYGTGVVRRIGNILDSGEDEWFLE
                     SADAFDETLFLIYRGNRSKSNSSRLVVKQLLGSVEDVFPLDSDTRAVSCLSDDTVALV
                     ERRNAQHA"
     gene            880..1524
                     /locus_tag="PKH07_14585"
     CDS             880..1524
                     /locus_tag="PKH07_14585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36210.1"
                     /translation="MSLSKRARLIWERHRFAVMLAAVLLLCCCLFFSLWQIEARRWST
                     DRIAGEPSMRWEILIRGVHDVPWSLIRVGLGSSAAQVDIIPSVWRLRLMLASKDPVTQ
                     ERGISIAYRDRNRLYIGEVEELLTAPDDTVFVLALLYLDSQEVTLNHASLLRLAEIAS
                     GRDEGSNAISLARSAVMRESVASATELQFDEFPADLVDALCEAMQSWVTLYDRV"
BASE COUNT          382 a          390 c          527 g          440 t
ORIGIN      
        1 tgcatacgta ggagatccgg agccggaaag acgtggaggg gtcatcgcga tcagcccaga
       61 tggtagtgcc tctgagttgg tacgaacgcc ttggatgcca agaggagcga ctctttcaag
      121 acggttcctc tataccgtgg aggccctacc ttacgtacta ccagatgaac ctgctggaga
      181 tggttcttat gtcggggtat ctgcggagat aaggttttgt cggcatgact gtagtcgcct
      241 ctacgaaact gccgatgtca ttgttctgag aggggaaagt gaaacatgtc gctggtttgc
      301 ggaattggca gcaaatcggg ttggaaatca agtgcttatc ttaccctcaa gacgatgtga
      361 agagatcgtg ttcatggtga gcacgggaac cggcgagata ctccaagtaa cctatgggac
      421 gggagtcgtc agaagaatag gtaacattct cgatagcggt gaagatgaat ggttcctcga
      481 gtcggctgat gcgttcgatg aaaccctttt cttgatatac cgtgggaata gaagcaagag
      541 taatagtagc agacttgttg tcaagcagtt gttgggttca gtagaggatg tttttcctct
      601 ggattctgat acacgcgcag tttcttgtct ctcagatgat actgtcgctc ttgttgagcg
      661 ccgaaatgct caacatgcat aggtgtctat cagattagca ggtagtaaac tgggttgagg
      721 gcaacccagg ctgagatctg ggatcgtcct tcggagcaaa gatggtgaaa cctggagaca
      781 ggttgaagcg ttcagcgcat attaacaaga caactgatgg gtcacgaccc atcctaccct
      841 gctggccaaa atgagttcgt tggtctgagg aggataccga tgtcattgtc taagcgtgct
      901 cgtttgatct gggagaggca tcgtttcgca gtgatgcttg ctgctgtgct gttgttgtgt
      961 tgttgtctct ttttcagtct ttggcagatt gaagcgcgcc gttggagcac ggatcggata
     1021 gcgggtgagc cgtccatgcg ctgggagatt ctgattcgag gcgtgcatga tgttccgtgg
     1081 agtctaatcc gcgtcggtct tggatcgagt gcagcacagg ttgatatcat cccctcggtt
     1141 tggaggctca gattgatgct ggcatcaaag gatcctgtga ctcaggagcg tgggatatcc
     1201 attgcgtaca gagatcgaaa ccgtctgtat atcggtgagg ttgaggagtt gctcacggca
     1261 cccgacgaca ctgtctttgt cttggctctt ctttatcttg acagccagga agtgacgctg
     1321 aatcacgcat cgcttctgag actggcggag atagcctccg gacgggatga ggggtcaaac
     1381 gccatctctt tggcgcgaag tgctgtcatg cgggagagtg tggctagcgc gacggagctg
     1441 caattcgatg agtttcctgc cgatctggtt gatgccttgt gtgaggcaat gcagtcctgg
     1501 gtgaccctct acgacagagt gtgattatat catgctggcc cccctttcac cgcggtgggt
     1561 ggcatggccg cttgtcggcc atgaaacagg cggacatgcc cgactagcgg gcatggcacc
     1621 cggtaagcca acggcgaaca gtccggttcc cacgccccct gaaccgtgcc acgatttgac
     1681 aacactcaca gttcagtagt agatatgagc aggttagcac ctgcgggcgc gtgggctat
//
LOCUS       DAOQUC010000969         1737 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_91144_length_1737_cov_2.240190, whole genome shotgun sequence.
ACCESSION   DAOQUC010000969 DAOQUC010000000
VERSION     DAOQUC010000969.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1737
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91144_length_1737_cov_2.240190"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..611)
                     /locus_tag="PKH07_14590"
     CDS             complement(<1..611)
                     /locus_tag="PKH07_14590"
                     /inference="COORDINATES: protein motif:HMM:NF013694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HNT36211.1"
                     /translation="MLRSFHYHAEKGIQTDLPLESFPELIKDNQNIIWVDMVEPTQKE
                     IKTVLAETFQFHPLAIEDCQTPAERPKAESYGSYLFVILPAEGLRFVDDEPDVTEIAC
                     FLGDNFLVTYHRRKLACFDVMAQRCQANPHHQMARGPAFIMHGIIDTLVDSYFPHIAK
                     VDNELDDLEQEIFQRPSEEILNKLLDARWRIQTLRRVLSPQRD"
     gene            complement(820..>1737)
                     /locus_tag="PKH07_14595"
     CDS             complement(820..>1737)
                     /locus_tag="PKH07_14595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36212.1"
                     /translation="TGAFKGTTSGTDPRIISQNGLALNKADWALIEIRMKASTGSFFD
                     LFFRGTADSVFSSAKRFRVTVEPGTQYVTYGIDTTLEPSWTGDVTQFRFDPVEVSGSS
                     VEIDYIRLYRTAPAATATKTPTNTPTTGLTNTPTRTPTNTPTNTPTNTPTNTPTNTPT
                     PLPTDVKWEFNTDGSFEGWSTVNMTSTQVSGGYFEGVSSTTAPRMTSADNLNWKASDR
                     VWIRVRMKVTAGNRARLFFRAPEDGVFSTSKMYELTVSDNSNFVVYNIDTTTHASWTD
                     IIRQIRLEPSNATSATIQVDYIYVYVTAP"
BASE COUNT          382 a          443 c          478 g          434 t
ORIGIN      
        1 atgtccctct gcggagagag cacccgacgc agcgtttgga ttctccaacg cgcatcaagc
       61 aacttgttca ggatttcctc agacggtctc tgaaatatct cctgttcaag atcatcaagc
      121 tcattatcta ccttggcgat atgcgggaaa tagctgtcca ccagtgtgtc aatgattccg
      181 tgcatgatga acgcaggccc acgcgccatc tgatgatgcg gattcgcctg acaacgttgc
      241 gccatgacgt caaaacaagc cagtttccgg cgatgatacg tcaccagaaa attgtctccc
      301 aggaaacagg caatctcggt cacatcgggc tcatcgtcaa caaatctcag cccttccgcg
      361 ggaaggatca caaacaggta cgaaccatag ctctccgctt tgggacgctc agcgggagtc
      421 tggcagtctt cgattgccag agggtgaaac tggaatgtct ccgccagaac ggtcttgatt
      481 tctttctgtg tcggctcaac catgtccacc caaatgatgt tctgattgtc cttgatgagt
      541 tccggaaaac tctcgagagg caagtccgtc tgaattccct tctctgcatg gtagtgaaaa
      601 cttcggagca ttgcgcaacc ttcttggaac agtctttctt cgaattttcc ggaaaaacgg
      661 cacgcgtcat cctatcgcca agcatgatat cactattcgc aaacaggaat caaacacgga
      721 tgttaaaata gtcattgacg ggtgcgggca gaaagggaga ggagcgctgg tgtgcgctcc
      781 tctccaagtg tctctgtaat gtgtgcgcca aacgcggcct tatggggcgg tgacgtagac
      841 gtagatgtag tctacctgga tcgttgcgct ggtggcgtta ctgggctcaa gtcgaatctg
      901 ccgaatgatg tctgtccaac tggcgtgagt cgtcgtgtct atgttgtaga cgacaaagtt
      961 gctgttgtcg gagacggtca actcgtacat cttggaagtt gagaagactc cgtcctccgg
     1021 tgctcggaag aacagccttg ctctgttgcc agccgtcacc ttcatccgaa ctctgatcca
     1081 gaccctatcg gaggccttcc agttgaggtt gtcagcggat gtcatgcgag gtgcagtggt
     1141 tgaactgact ccctcgaagt atccacccga cacctgtgtg gaggtcatgt tgactgttga
     1201 ccaaccttca aaactgccat cggtgttgaa ttcccacttc acgtctgtcg gaagtggcgt
     1261 cggcgtgttg gtcggggtgt tggtcggagt gttcgtcggt gtattggttg gcgtattcgt
     1321 cggcgttctg gtcggggtat tggtcagacc agtggtcggt gtgttcgttg gtgttttggt
     1381 tgccgtggca gccggagcag tgcgatagag ccgaatgtag tctatctcaa cactggaacc
     1441 ggagacttca accggatcga accggaactg agtgacatcg cccgtccaac tcggctcaag
     1501 tgtggtgtca ataccgtaag tcacgtactg tgtgccgggc tcgacagtga ctcggaatct
     1561 cttcgcagaa gaaaagaccg aatccgccgt ccctcggaag aacaggtcaa agaagcttcc
     1621 ggtggaggct ttcattcgga tctcgatcag cgcccagtcc gctttgttca aagcaaggcc
     1681 gttctgcgag atgattcgag gatctgttcc cgaagtagtt cccttgaaag cgccagt
//
LOCUS       DAOQUC010000970         1737 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_91148_length_1737_cov_2.162307, whole genome shotgun sequence.
ACCESSION   DAOQUC010000970 DAOQUC010000000
VERSION     DAOQUC010000970.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1737
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91148_length_1737_cov_2.162307"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..756)
                     /locus_tag="PKH07_14600"
     CDS             complement(<1..756)
                     /locus_tag="PKH07_14600"
                     /inference="COORDINATES: protein motif:HMM:NF013673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HNT36213.1"
                     /translation="MNDLLIEMRDYLKRRGADYADLRFESAMRESVEVRNGKVERVSS
                     SQDQGVGVRVLLGGGWGFAATSESSRQALRQTSNLAMEVARASALVSRHKVILAQQAP
                     QKGEYRSPCEKDPFEVPLSVKIDLLMRACDTIQRDERIRVARGNIRWSKTRKWFLNTE
                     GTETQQEIIVSAAGIEATAVSGNDAQKRSYPAGHEGDAATRGFEFIEQMNLPGNAVRV
                     REEALMLLDAPECPAGAQDVILSGSQMALQIHES"
     gene            complement(769..>1737)
                     /locus_tag="PKH07_14605"
     CDS             complement(769..>1737)
                     /locus_tag="PKH07_14605"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HNT36214.1"
                     /translation="LTAILLMLKRKGTDFCSLDELAETTQTTPESVADDLSFLQEQHY
                     NIETRPSLGVRLIDMPDVVYPHEVLSEIKDSRWLNGSLDILYSPEIASTNEAAKQLAR
                     QDAPDGTVVVTDYQTKGKGRQGRQWVSPPGTNLLFSIVLRPAIVARRLPFLTVMSSTA
                     VAAALRDEFGLAVGLKWPNDLVLDGKKLGGILTEGEGLEGNVHFAVIGIGLNVNCQTE
                     SLPREIAINATSLRDALGYPVHRAHVLAVVLARLKRFYEELLFGSSEVILDMCKQLSD
                     TIGKFVQISSSTSGYAEDIDQEGALVLRLENGMRKRVWVGDLTQLF"
BASE COUNT          388 a          498 c          452 g          399 t
ORIGIN      
        1 ggattcgtga atctgcagtg ccatctggga gccactcaga atcacatcct gcgcacctgc
       61 aggacactcg ggtgcgtcca ggagcatgag cgcctcttca cgaacccgaa cggcgttgcc
      121 aggaaggttc atctgttcta taaactcgaa accccttgtt gctgcgtcac cttcatgacc
      181 cgcaggatag gagcgttttt gcgcatcatt gccggaaacg gcagtggctt cgatacctgc
      241 ggccgaaaca atgatctcct gttgtgtttc ggtgccttcg gtattgagaa accatttgcg
      301 agtctttgac caacggatgt ttcctcgtgc aactcggatg cgctcatctc gttggatcgt
      361 gtcacaggcg cgcatcagaa ggtctatctt gacagacaga ggaacctcaa agggatcctt
      421 ttcgcacggg gaacgatact cacccttctg cggcgcttgc tgagccagaa tcactttgtg
      481 tcgggaaacc agggcacttg cccgcgccac ttccatcgcc aggtttgagg tttgtcgaag
      541 cgcttgtcgg gatgactccg acgtggcagc aaaaccccag ccgcctccga gaagtactcg
      601 aactccaaca ccctgatctt gactgctgct gacgcgctcc accttgccgt tgcggacttc
      661 gacggactca cgcatcgcgg actcaaaccg gagatcggcg taatccgcac cgcgtctctt
      721 aagatagtcc ctcatctcga taagaaggtc attcatcggc tccaccccct agaaaagttg
      781 agtcaggtct ccgacccaga ctcgtttgcg cattccgttt tccagtctga gcaccaacgc
      841 tccctcctga tcaatgtcct ctgcatagcc cgacgtggaa gaactgattt gaacgaactt
      901 cccaatggtg tcagacagct gcttgcacat gtcaagaatc acttctgacg agccgaagag
      961 cagttcctcg tagaatcgct tcagtcgggc aagcacgacc gccaggacat gtgccctgtg
     1021 aactggatat cccagagcat ccctgagaga tgtggcgttg atcgcaatct cacgtggaag
     1081 agattccgtt tggcagttga cattgaggcc gattccgatg acggcaaagt gaacgtttcc
     1141 ctcaagtccc tctccctcgg tgaggatgcc accgagtttc tttccatcga gaacaagatc
     1201 gttcggccat ttcaacccca ctgccaggcc gaactcatcg cgaagagctg cggcaaccgc
     1261 cgttgaactc atgaccgtca gaaaaggaag gcgacgcgct acaatcgcgg gtctgaggac
     1321 aatcgaaaag agaagatttg tgcctggggg tgacacccat tgtcgccctt gccgtccctt
     1381 gccttttgtc tgatagtccg tgacaacaac ggttccgtca ggggcgtcct gccgtgccag
     1441 ttgtttcgcg gcttcattgg ttgaggcaat ctccggagag taaaggatgt cgagggaacc
     1501 gttgagccac cgggaatcct tgatctcgga aagcacttcg tgaggataga caacatccgg
     1561 catatcgatc agtcggaccc ccaatgacgg gcgagtctcg atattgtagt gctgctcctg
     1621 aaggaaactc agatcatcag caacagactc aggagttgtc tgtgtcgtct ctgccagttc
     1681 atcgagcgag cagaagtcag tcccttttct cttcagcatc agcagaattg ctgtcag
//
LOCUS       DAOQUC010000971         1735 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_91319_length_1735_cov_2.422619, whole genome shotgun sequence.
ACCESSION   DAOQUC010000971 DAOQUC010000000
VERSION     DAOQUC010000971.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91319_length_1735_cov_2.422619"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1075
                     /locus_tag="PKH07_14610"
     CDS             <1..1075
                     /locus_tag="PKH07_14610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020249519.1"
                     /note="catalyses the hydrolysis of terminal non-reducing
                     alpha-D-galactose residues in alpha-D-galactosides;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HNT36215.1"
                     /translation="TIPVVLEIAKDVMELCPNALVLNYVNPMAMICWALGTVPGLKFI
                     GLCHGVQTTLDLIAGYVGARKDEIDFLCAGINHMAWFLKLEKQGTDLYPVLRECFEKP
                     EYYINEKVRGEVFRHFGYFMTESTGHLSEYVPWFRSSERALKLYCDQPAFGGESGAYY
                     KWGRLIADKFSGADVLSQESKKLTPRSVEYCSYIVEAVETGRIFKLNGNVRNEGFIKN
                     LPEGCCVEVPIFVDKQGLHPTGVGMLPSQCAALNMTNVTVQGLTVEAAVTGDPELVMQ
                     AASLDPLTSAVLTLNETREMTRELLQAESEYLPQFAGKTLRSCPTILTPPETKAVEAP
                     LDPALAIVHRFGSLADKAMHKKG"
     gene            1103..>1735
                     /locus_tag="PKH07_14615"
     CDS             1103..>1735
                     /locus_tag="PKH07_14615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012508310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015655 - alanine:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0032328 - alanine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HNT36216.1"
                     /translation="MSEFLLALEGLLGKVSNIVWGPALLILLFGTHLFLTFRLRFIQR
                     YLWKAIKLSFTKGGEGAGDISHFGALATALAATIGTGNIVGVATAISLGGPGAVLWMW
                     LTGVFGIATKYAESLLSVKYRVKTERGTMAGGPMYVLERGMKMKWLAVLFALFAAVAA
                     FGIGNMAQANSISQMLQNSYSIQPWISGVVLACLTAIVVLGGIRSIARVCE"
BASE COUNT          370 a          431 c          511 g          423 t
ORIGIN      
        1 gacgattccc gttgtcctgg aaattgccaa agacgtcatg gaattgtgtc cgaacgcact
       61 ggttctgaac tatgtcaatc caatggcgat gatctgttgg gcacttggca ccgtgccggg
      121 tttgaagttc atcggactct gccatggagt gcagacgaca ctggatttga tcgcgggata
      181 tgtgggagca cgcaaggacg agatcgactt cctttgtgcc ggaatcaacc acatggcgtg
      241 gtttctcaag ctggagaagc agggcacgga tctgtatccc gtccttcggg agtgcttcga
      301 gaaacccgag tactacatca atgagaaagt tcgtggcgaa gtgttccgtc acttcggcta
      361 cttcatgacg gagagcaccg gtcacctttc ggaatacgtt ccgtggttcc gaagcagtga
      421 acgggcgctc aaactgtact gcgatcaacc cgcttttggt ggtgagagcg gtgcttacta
      481 caaatggggt cgcctgatcg ccgacaaatt cagcggcgct gatgtcctga gtcaggagtc
      541 gaagaagttg acgcctagaa gcgtcgagta ctgctcatac atcgttgagg cggtcgagac
      601 gggtaggatt ttcaaactca atggcaacgt tcgcaacgaa ggattcatca agaaccttcc
      661 ggaaggctgc tgcgtggaag tgccgatttt tgtggacaaa caggggcttc atcccaccgg
      721 tgttggaatg cttccatcgc aatgtgcagc attgaacatg accaacgtca cggttcaagg
      781 tctgaccgtt gaggctgccg tgacaggcga tccggaactg gtcatgcagg cggcctctct
      841 cgatccgctg acgtccgcgg tcttgactct gaacgaaacg cgcgagatga cacgggaact
      901 gctccaggcg gagtccgagt atctgcctca gtttgccgga aagacgcttc gctcctgtcc
      961 gaccattttg acgccacccg aaacaaaagc agttgaagcg cctctggatc cggcgctggc
     1021 catcgtgcat cgtttcggct ctctcgctga caaagccatg cacaagaagg gatagtccaa
     1081 ccagaagggg gtacgagttg tcatgagtga gtttctgctt gcgttggagg gattgctcgg
     1141 caaagtctcg aacattgtct ggggaccggc acttttgatc ctgttgtttg ggacgcacct
     1201 gtttctcacg ttccgacttc gcttcatcca gcgatatctc tggaaggcga tcaaactttc
     1261 tttcaccaaa ggtggtgaag gtgcgggaga tatcagccat ttcggtgctc tggcgacggc
     1321 tcttgcagcg accataggca ctgggaatat tgtcggtgtg gcgacagcga tatctctcgg
     1381 agggcctggc gcggtgttgt ggatgtggct gaccggtgtg ttcggcattg ccaccaagta
     1441 cgcggaatca ctcctgtctg tgaagtaccg tgtcaagacg gaacgaggca cgatggcagg
     1501 tggaccgatg tatgttctcg aacgcggcat gaagatgaaa tggcttgccg tattgttcgc
     1561 gctctttgct gccgttgctg cttttggtat cggcaacatg gcacaggcaa actccatttc
     1621 ccaaatgctg caaaacagtt attccatcca gccttggata tcaggagttg tgctggcttg
     1681 tctgacggcc atcgtggttc ttggtgggat tcgttctatc gcccgggttt gtgaa
//
LOCUS       DAOQUC010000972         1734 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_91371_length_1734_cov_3.532460, whole genome shotgun sequence.
ACCESSION   DAOQUC010000972 DAOQUC010000000
VERSION     DAOQUC010000972.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1734)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1734)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1734
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91371_length_1734_cov_3.532460"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            286..603
                     /locus_tag="PKH07_14620"
     CDS             286..603
                     /locus_tag="PKH07_14620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36217.1"
                     /translation="MEKGTLAVFENYRIRRHFDEETQTWYFSVVDIVRVLVEQPDYNR
                     ARKYWNKLKERLGKEGSESVTNCHQLKLEAADGKKYLTDVADPETLLRLIQSIPSVGQ
                     YYV"
     gene            596..748
                     /locus_tag="PKH07_14625"
     CDS             596..748
                     /locus_tag="PKH07_14625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36218.1"
                     /translation="MSKQISEEEYDFTINCDIKYRMGQEADSEIATSDTRRTRYFEIE
                     SNGQWR"
     gene            complement(914..1333)
                     /locus_tag="PKH07_14630"
     CDS             complement(914..1333)
                     /locus_tag="PKH07_14630"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNT36219.1"
                     /translation="MAKILIVDDEEDIAEVIRITLETSGHIVQTASNGAEGLTFVKKI
                     EPDLVILDVMMPKMNGMQVVEILKGSDATAHIPILMLTAVTKNSLQPDEHWRKKVGVE
                     DFMSKPFEPLELRDRVDKMLKERYREKGDGFTRYRIG"
     gene            complement(1394..>1734)
                     /locus_tag="PKH07_14635"
     CDS             complement(1394..>1734)
                     /locus_tag="PKH07_14635"
                     /inference="COORDINATES: protein motif:HMM:NF020091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HNT36220.1"
                     /translation="SGSTIEPVATVRMRKGDQEHSEASCGDGPVDAVYKAIRKITNTQ
                     SQLTTYQLKAVTGGTDAQGEVRVVLEEDGLKASGVGTDTDVIIASAKAYVNAINRLLY
                     MKSRRAQNAK"
BASE COUNT          393 a          448 c          474 g          419 t
ORIGIN      
        1 tgagcagggg catagcccac gcgccagcag gcgcttgcct gctcggaggg agttccgtca
       61 acctccgttc aaaggtctat cgcgactccg atggagccaa gttcttctct actggtttgc
      121 tgatctgcga gagaacagga taaccaagct aacaggacgt aacctggccg aatctgcgct
      181 aagcgtactg agattgacag taccggagtt ggacttcatc atcaaccacg ataccaagta
      241 ttggatcagg caacgggcgg aaggagaaga gcaaggagtg cagatatgga aaagggaacg
      301 ttggcagtgt ttgaaaacta caggattcgc cgtcatttcg atgaagagac gcagacatgg
      361 tatttctcgg ttgttgatat agtgcgggtt ctagtagaac aaccggacta caatagagcc
      421 aggaagtatt ggaacaagct caaagagcgc ctcggaaagg aaggcagtga gtcggtgaca
      481 aattgtcacc aactgaaact ggaggcggcg gatgggaaga agtacctgac tgatgttgct
      541 gatcctgaga ccttgttgcg tctcattcag tccattccca gtgtagggca atactatgtc
      601 taaacagatc tctgaagagg aatacgactt caccatcaac tgcgatatca aataccggat
      661 gggacaggag gcagacagtg agatagccac gagtgacacg cggcgaactc ggtatttcga
      721 gatagaaagc aatggccaat ggcgttgatc gtacttggag ccggcgcgcc acgcggagcg
      781 tctttttgag aaaccgcagg gcccgatcca tctgtcttcc gccctcggat cttgatacct
      841 ggaaatagaa accgctccga cattcggaag aacatcggag cggtctattt gttatgttct
      901 gagaaggggc gcgttatccg attcggtatc gggtgaaacc gtcgcccttc tcgcgatagc
      961 gttccttgag cattttgtct acgcggtcgc gcagttcgag tggctcaaag ggtttggaca
     1021 tgaagtcttc gacgccgacc ttctttctcc agtgctcatc gggttgaagc gagttcttcg
     1081 tcaccgccgt cagcatcagg atcggaatgt gagcagtcgc gtcgctgcct ttgagaatct
     1141 cgacaacctg catcccgttc atcttcggca tcatgacatc gagaatgacc aggtccggct
     1201 cgattttttt cacaaaggtc agaccttccg ctccattgct ggcggtctgg acaatgtgtc
     1261 cactggtctc cagcgtgatg cgaataactt cagcgatatc ttcttcgtca tccacgataa
     1321 gaatcttcgc catgttgtcc acccctttcg tcgaatgagc gtcggagtcg tctccgtgct
     1381 cctgaacgac aggttacttt gcgttttgtg ctcgacgcga cttcatgtag agaagccggt
     1441 tgatcgcgtt cacataggcc ttggcactcg cgatgatgac gtcggtatcc gtcccaactc
     1501 cggaagcctt cagaccgtcc tcttcaagaa caacccgaac ttcgccttgg gcatcggttc
     1561 cgcccgtcac tgccttcagc tgataggttg tcagctggct ctgcgtgttg gtgatcttcc
     1621 tgatcgcttt gtagacggca tctacggggc cgtcgccgca gctggcttca ctgtgttcct
     1681 gatctccttt gcgcatccga accgttgcga ccggttcaat ggtgctgcca ctgg
//
LOCUS       DAOQUC010000973         1732 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_91520_length_1732_cov_3.667859, whole genome shotgun sequence.
ACCESSION   DAOQUC010000973 DAOQUC010000000
VERSION     DAOQUC010000973.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1732)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1732)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1732
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91520_length_1732_cov_3.667859"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            156..437
                     /locus_tag="PKH07_14640"
     CDS             156..437
                     /locus_tag="PKH07_14640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36221.1"
                     /translation="METLSLLFRVMADALPSGPTLGLLLVILLYLLYRRTRFTLLFIF
                     TFACFWSGIQFSVNEQLKVQSNVAWTFVCALLGFVGVIYGIYVFTQGES"
     gene            462..1403
                     /locus_tag="PKH07_14645"
     CDS             462..1403
                     /locus_tag="PKH07_14645"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HNT36222.1"
                     /translation="MDRRQFIRNTGTLLGAGLCGIGLGKQRGWAEESTAAATPAPEKS
                     DSASKLVVVRGADTRALVHKSLEALGGIEQYVKSGNVVVVKPNIAWDRTPEQAANTHP
                     EIVAAVVALCMKAGAKQVKVFDNTCNDARRSYVTSGIEEAAKKEGATVNFVDERLFRK
                     VAFPKGKLVQEWEVYGEALDADVFINIPIAKHHSLSKLTMCMKNHFGVMGGRRGLLHQ
                     DIDQRIADLSTLLKPQLSILDATRILKANGPTGGNLEDVQVLNTLIASTDPVAVDSYG
                     ATLFGLKGDEIGYIKIANEMGLGKMYPSEIQALELAV"
     gene            1569..>1732
                     /gene="ssrA"
                     /locus_tag="PKH07_14650"
     tmRNA           1569..>1732
                     /gene="ssrA"
                     /locus_tag="PKH07_14650"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT          403 a          420 c          487 g          422 t
ORIGIN      
        1 acgctgactt gttttgtttc aggctgccga aaacttaccc gaaaaaccgc ctgactcttt
       61 cgaactggct attcttggtt gcagccggac gacgcaatcg ctataatcca aatctgagtg
      121 tcgccaagtc tgcttaggtc ggaggtgcgt cgcgtatgga gacattgagc ctgctgttcc
      181 gtgtcatggc agatgcccta ccctcaggac ccacgttggg gcttctcctg gtgatccttc
      241 tttatcttct gtatcgaaga acgcgtttca cgttgctctt tattttcacc tttgcctgtt
      301 tctggtctgg gattcagttc tcggtcaatg agcagctgaa ggtgcaatcg aatgtcgcgt
      361 ggacgtttgt atgcgccctt ttgggttttg tcggtgtgat ctatgggatt tatgttttca
      421 cgcagggaga gtcgtaatcg cccttctgga ggaacgcgag tatggataga agacagttca
      481 ttcgcaacac aggcactttg ctcggtgccg gtctgtgtgg aataggcttg ggcaagcaac
      541 gaggttgggc tgaggagagc acagctgccg caactccagc tcctgagaag agcgattctg
      601 cttcgaaact cgttgtcgtg cgaggagcgg acacccgggc tttggttcac aaatccctcg
      661 aagcactggg gggaatcgag caatacgtca aatccggcaa tgtcgtggtg gtgaaaccca
      721 acatcgcttg ggacagaacc ccggaacaag cagccaacac gcatcctgag attgttgcgg
      781 ctgttgtcgc gctgtgcatg aaagcgggag ccaaacaggt gaaggttttt gacaacacct
      841 gcaatgacgc ccgaagaagc tatgtgactt ccggtatcga ggaagccgcc aagaaggaag
      901 gcgcgaccgt caactttgtg gatgagcggc tctttcgcaa agtggcgttt ccaaagggaa
      961 aactggttca ggaatgggaa gtctatggcg aagcgttgga tgctgatgtg ttcatcaaca
     1021 ttcccattgc caagcaccac agcctgagca aactcaccat gtgtatgaag aatcattttg
     1081 gtgtcatggg gggacgccgc ggcttgcttc accaggacat tgatcagcgc attgccgact
     1141 tgagcacact tctcaagcca cagctgagca ttctggacgc cactcggatt ctcaaagcca
     1201 atgggccgac aggtggaaac ctggaagacg ttcaggtact caacacgctg atcgcaagta
     1261 cagatccggt tgcggtagac agttacgggg cgaccctttt cggactcaaa ggcgacgaaa
     1321 taggctacat caagatcgcc aatgaaatgg gactggggaa gatgtacccg agtgagattc
     1381 aggcgctcga attggctgtc tgatggtctc agcgtctgta ctgctgcggc ttcgtgcaga
     1441 gatgagaaga atcatgggga gtaaccaaat cccctcttcg tccgttcaag agtaagaaac
     1501 ctcaactgat gatttccacc cagtatctcc ttgtccaagg caaggttctg ttgaggcttt
     1561 cagagactgg gggcgtaaag gtttcgacgg aggtgattag tcagagtaga tgcctgtcgg
     1621 ggtttccctt tgcctcgtta ataatgaagg gaaataagtg taactgcaaa cgaaacactt
     1681 ccactggctg cctaatcgtg tagccacgct cccttgacct gggctccttc gg
//
LOCUS       DAOQUC010000974         1728 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_91930_length_1728_cov_2.286312, whole genome shotgun sequence.
ACCESSION   DAOQUC010000974 DAOQUC010000000
VERSION     DAOQUC010000974.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1728)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1728)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1728
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91930_length_1728_cov_2.286312"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..989
                     /locus_tag="PKH07_14655"
     CDS             <1..989
                     /locus_tag="PKH07_14655"
                     /inference="COORDINATES: protein motif:HMM:NF021021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M64 family metallopeptidase"
                     /protein_id="HNT36223.1"
                     /translation="FTQEPWNEYQSYVNVYAIEVISNESGADHPSLGIYKDTALGATY
                     DFGGVRRALFCTETQSVFNVANRIPGWDAVYIVVNDTEYGGTGGYYSTFSVHPDGPDV
                     AVHENAHTFAGLADEYEGDMGGGKDSEPNIENATTLEAIKWKMWILPDTPIPTPDESL
                     YDGLVGLFEGAKYQMTGLYRPKRNCKMRELGVAFCEVCTETHLVGSYARSIYKILDII
                     EGTTPSEQDFEVTLGGTSSLLFSLDTLQNESQTVKARWYLDGVEQLGVSDTQISISGS
                     QLSQGDHWVYVIVRDRTDMIRNDPFNYRREEYYWTFKVNLVSSVAPQIWNSY"
     gene            1460..>1728
                     /locus_tag="PKH07_14660"
     CDS             1460..>1728
                     /locus_tag="PKH07_14660"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic peptidoglycan transglycosylase"
                     /protein_id="HNT36224.1"
                     /translation="MLVSEDDAFYSHNGFDYNQVREAIKESWDERRLTRGASTITQQL
                     AKNLYLSPSRNPLRKIKEIIITKRLEKALSKDRILEIYLNVVEWG"
BASE COUNT          465 a          472 c          420 g          371 t
ORIGIN      
        1 tgttcacgca ggagccgtgg aacgaatacc agtcctacgt caatgtctat gcgatcgagg
       61 ttatctccaa tgagtccggc gcggatcacc cgagtctggg catctacaaa gacaccgccc
      121 tcggagccac ctacgatttt ggaggagtga ggcgggcgtt gttctgtacc gaaacgcagt
      181 cggtattcaa tgtggcaaac cgcattccgg gatgggatgc agtctacatc gttgtcaacg
      241 acaccgaata tggtggcaca ggagggtatt attccacctt ctccgttcat ccggacggac
      301 ccgatgtcgc cgttcatgaa aatgctcata ccttcgccgg actggctgat gaatatgagg
      361 gagacatggg cgggggcaaa gactcagaac ccaacatcga aaatgccaca acactcgaag
      421 caatcaaatg gaaaatgtgg attctccccg acactcctat tcccacccct gatgagtctt
      481 tgtatgatgg ccttgttggc cttttcgaag gagccaagta ccagatgacc ggcctttaca
      541 gaccgaaacg caactgcaaa atgcgggaac tcggagttgc tttctgtgaa gtgtgcactg
      601 agactcatct ggtgggcagc tatgctcgca gcatctacaa gattctcgat atcattgaag
      661 gcaccacacc atcagagcag gacttcgagg taacgcttgg cggaacaagc agtctgctgt
      721 tctctctcga caccctgcaa aatgagtcac aaacggtgaa agcacgttgg tatcttgacg
      781 gagtggaaca gctgggcgtt tccgataccc agatatcaat ttcaggctct cagctgtccc
      841 agggcgacca ctgggtttac gtcattgtca gggatagaac agacatgatc cgaaatgacc
      901 cattcaacta ccggcgcgaa gagtattact ggacgttcaa agtaaatctg gtttcttccg
      961 ttgcccccca aatatggaac tcctactagc ttcagcccaa gcacgagatg ctgtatctga
     1021 cctctccaaa tgccgcagga cttgcattct ggttggagaa tcaaccacac agatgatcct
     1081 gacgaacaag gagagaacaa agccccatcc aacctccaag tcctcaaaga agaaggcaac
     1141 tggcgcaaca tccaaacgag tccagcatac gaaaggccgc catgagggaa cacgtgtgcc
     1201 caaaaatcgg acaaaccgtc ccgaagtgtc aggccgaagt ttcttctttt ggagttccgt
     1261 cgccctcgtt ctcctcgctg ctttcctcgc gttcgaattc ctgacacttc caaatccctc
     1321 cgagcttcgc acggtgaacc ctactcgaac cgcattcatg aaacgctctg ataaacaacg
     1381 cgcccgcaaa ggcctttccc catcacgcac aggacagtgg acacgtcttt cgtccgtttc
     1441 catatacttg agaaacgccg tgctcgtgtc tgaagatgat gcgttctact cccacaacgg
     1501 atttgactac aatcaggttc gagaggcgat caaggaaagc tgggatgaga gaagactgac
     1561 acgcggcgca agcaccatca cacaacaact ggcaaagaac ctctacttga gtccttccag
     1621 aaatccactt cgcaagatca aggagatcat catcaccaag agacttgaga aggcgctgtc
     1681 aaaggataga atccttgaga tctacctgaa cgtggtcgag tggggaga
//
LOCUS       DAOQUC010000975         1726 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_92073_length_1726_cov_3.350688, whole genome shotgun sequence.
ACCESSION   DAOQUC010000975 DAOQUC010000000
VERSION     DAOQUC010000975.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1726
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92073_length_1726_cov_3.350688"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1456
                     /gene="mtaB"
                     /locus_tag="PKH07_14665"
     CDS             <1..1456
                     /gene="mtaB"
                     /locus_tag="PKH07_14665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HNT36225.1"
                     /translation="RQGVLRVTFHQHRAVIRSLGCKVNQCDAEATASELRAAGCVILD
                     DSDATSLDESPADLCIVYTCSVTAEGDRKSRQMIRHLIREHPNALMVVAGCYAEVRPD
                     DLRTIDGVDMVVGTSDAAQLLPQIYAALDIMEPDGEKSCSEDSCRRTRAFVKIQDGCD
                     QFCSYCQIPLTRGTPHSRDTGTILKEVRGFVEHGHLEVVLCGIRLGAFGMDRGDPNAL
                     AELLRQLDTIEGLVRYRLSSIEPNDFGEPLLKALPELKKLAPHFHIPLQSGSDTVLQR
                     MNRRYTAQDYQSLIKRLQSLIPNLAVSTDVLVGFPGETEEEARETEVFVEKMLFSKVH
                     LFPFSPRPGTVAADLKPRISAADVRCRKRRLEGVTLAGAIQFRKRFVGSTLQVLIEGK
                     ELVASETGKMFRTGFSENYLRVAIPTSCEQPLRAGEVYDTVIERLDRNMLLKTQTSED
                     VLSSSEERFEKVGTAQESTGAGLLIGNVPIASNR"
     gene            1487..>1726
                     /locus_tag="PKH07_14670"
     CDS             1487..>1726
                     /locus_tag="PKH07_14670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36226.1"
                     /translation="MQKRLMTRQQLKEVLVRNQCAFDEIFGHEGQSALLLQRGARIIG
                     LFPDSDPDAINVLWVNPLLERLLTGKIQDWVEEGMG"
BASE COUNT          390 a          437 c          495 g          404 t
ORIGIN      
        1 ccggcaagga gttttgagag tgacatttca ccagcatcgg gctgtgattc gatccttggg
       61 ctgcaaggtc aatcaatgcg atgcggaagc gacggcttcc gagttgaggg cagcaggatg
      121 cgtgattctc gatgattctg atgccaccag tttggatgaa tccccagccg acctttgtat
      181 tgtctatacc tgttctgtga cggcggaagg ggatcgaaag tcccgacaga tgattcgcca
      241 cctgattcgc gaacacccca acgccctgat ggttgtagcg ggctgctatg cggaagtccg
      301 accagacgat ttgaggacaa ttgatggcgt ggacatggtg gtcggaacgt ccgatgcagc
      361 acaactgcta cctcaaatct acgcggcgct cgacatcatg gagcctgacg gagagaaatc
      421 ctgctcggaa gattcatgtc gacgcactcg cgcatttgtg aagattcaag acggctgtga
      481 tcagttttgc agctactgcc aaatcccttt gactcgtggg acgcctcaca gccgcgatac
      541 cggcactata ttgaaagagg tgcgtggctt cgttgaacac ggtcatcttg aggtggttct
      601 ctgtggcatc cgcttgggtg cattcgggat ggacaggggc gaccccaacg cgctcgctga
      661 gctgcttcga caactggaca ccatcgaggg acttgttcgt taccgactca gttccattga
      721 accgaatgat tttggcgagc ccctgctgaa agcacttccg gagttgaaaa aactcgcgcc
      781 tcactttcat atcccgcttc agagcggctc cgataccgtt ctgcaaagaa tgaaccgtcg
      841 ttacaccgct caggactatc agtccctgat caaacggctt cagtctttga ttcccaattt
      901 ggcagtctcg acggacgtgc tcgttggttt cccgggtgaa acggaggagg aagcacgaga
      961 gacagaagtc tttgtggaga agatgctctt ctccaaagtc catctgttcc ctttctcgcc
     1021 tcgtccgggg acggtggctg cggatctgaa accacggatt tccgccgcgg acgttcgctg
     1081 tcgaaagaga cgtcttgagg gagtaactct tgccggagcg attcagtttc ggaaacgctt
     1141 tgtcggttcc acactccaag tgcttatcga gggaaaggaa cttgttgctt ccgaaaccgg
     1201 gaagatgttt cgaaccggct tttcggagaa ctaccttcgc gtggcgattc cgacaagctg
     1261 cgagcaaccc ttgcgagcgg gagaggttta cgatacagtc attgagcgat tggatcgcaa
     1321 catgctgctg aaaactcaga cttcagagga tgtgctttcc tcttcggagg agcgatttga
     1381 gaaggtgggc acagcccagg aatctacagg cgctggcctg ctgatcggga acgtcccgat
     1441 agcgtccaat cgttgagcca ttttgacgat tgtttggagg gtgactatgc agaagagact
     1501 gatgacacga caacaactca aagaagtcct ggtacgaaat cagtgtgcct ttgatgaaat
     1561 cttcggtcac gagggtcagt cggcactcct gctccagcgc ggggcaagaa tcattggatt
     1621 gtttccggac agtgacccgg atgccattaa cgtactctgg gtcaatcctc tcttggaacg
     1681 actgctcacc ggtaagatac aggactgggt cgaggagggg atgggc
//
LOCUS       DAOQUC010000976         1725 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_92167_length_1725_cov_3.080838, whole genome shotgun sequence.
ACCESSION   DAOQUC010000976 DAOQUC010000000
VERSION     DAOQUC010000976.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1725)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1725)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1725
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92167_length_1725_cov_3.080838"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(696..785)
                     /locus_tag="PKH07_14675"
                     /pseudo
     CDS             complement(696..785)
                     /locus_tag="PKH07_14675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003112842.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
     gene            1033..>1725
                     /locus_tag="PKH07_14680"
     CDS             1033..>1725
                     /locus_tag="PKH07_14680"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNT36227.1"
                     /translation="MMRKTNKFSQAIFILVAICLVVSGCQKRPPDESPNVLILLMDTL
                     RADHLGYQRYELNTSPNLDAFARENSFLEPFYTVAPWTSPAVASLFTGLYPSSHGVLG
                     HPDLLDGETRVEFRMLPESANTLAEFFRSQGYRTAAVSSNPWVLKEFGFDQGFEQFVV
                     LPNKAPAEAVNAEAFKYLDSISKTKRPFFLYVHYMDCHGMYVPPKPYNEMFLPEKPQD
                     EQLVLPKNLVPKY"
BASE COUNT          415 a          453 c          436 g          421 t
ORIGIN      
        1 gcatcatcgc ccaaagccat acataaggca actcaattgg ctgagcgctc agtgtgcacc
       61 aagtgccttc aatatctaca accgttttga tcggattttg gtaactgttt cgctcccgag
      121 cacatcaccc caagtcatcg agccgtgtgt atatttccag gtccgaaaat gtgaatgtcc
      181 ccatcggaga tggtgccgtt ggacggatcg tatcccaccg ccgtaggaag tgaatgattg
      241 gcatctgcgt ctgtgtccag ccacttgttt ggtcccacac tgagcaccat atatttgagc
      301 ccacctttgg gggttccata ggcaacttca tagacatcac tcaggtagaa ttttgctcgg
      361 ttgccggacg tctgcaactt ctcattgtgc gcttgctcgc tgtagaagaa acgcccccag
      421 tgtgggtaaa tgctcgggtt ggtaaagacg tccctcggat acggtttcat gtaagccaca
      481 ggcgttgtaa ttctgcctgg aattctgccc aacggccaaa gattgatacc aaagaaagga
      541 tctctcacgg gatcgcctgt gaccgtgttg gaattgtaga gataagaggg tggcggatag
      601 cagccgttgt caacgtagta cgactctatc gggataagag agttgcgcag atccgccttg
      661 gcacgagcca ccttcgcccg aacttgtgcc aagagaaagt tcggaatcgc gatcgccgcg
      721 agaatcccga tgatggcaac aacgatgagc agctcgatga gtgtgaagcc cttgcttgct
      781 ttcatgattt gttccccttt ctcaatctcc tttgaggcca aaaacgcgag acaccaaaac
      841 ccaatatcga atccaataag ccgtatgatg cgctgttcgg aagcagttcc aggcagaggc
      901 accaccaagt catagtatac caccgttccg ttggtatgtc taaaattcca gaaatccacc
      961 aaacggtgtg gctttgtcgc agaagctgtg gtattttatg agctggaaaa ataacgaagg
     1021 gttcttgagc agatgatgag aaagacgaac aagttcagtc aagccatatt cattttggtg
     1081 gcaatctgtc tggttgtctc gggttgccag aaacgcccgc ctgacgaaag cccaaacgtg
     1141 ctgattctcc ttatggacac gcttcgggca gaccatcttg gctatcagcg ctacgagctg
     1201 aacaccagcc ccaatttgga tgcttttgcg cgggagaatt cctttctgga gcctttctac
     1261 actgtcgcgc cctggacctc tccggcagtg gcctcactct tcacgggtct gtacccctct
     1321 tcgcacggag tgctgggtca tcccgatcta ttggacggtg agacgcgtgt ggagtttcga
     1381 atgctgccgg aatccgcgaa cactctggcg gagttcttcc gctcccaggg atatcgaacc
     1441 gcggcggtca gttcgaatcc gtgggttctc aaggaattcg gattcgatca aggatttgag
     1501 cagtttgtcg tgttgccaaa caaggctcct gccgaggcag tcaacgcgga ggcattcaag
     1561 tatctcgaca gcatctccaa aaccaaaaga ccgttctttc tctacgttca ttatatggac
     1621 tgccacggta tgtatgtgcc acccaaacca tacaatgaaa tgttcctgcc cgagaagcca
     1681 caggacgagc aactggtact gcccaagaat ctcgtgccca agtat
//
LOCUS       DAOQUC010000977         1724 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_92261_length_1724_cov_3.168963, whole genome shotgun sequence.
ACCESSION   DAOQUC010000977 DAOQUC010000000
VERSION     DAOQUC010000977.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1724)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1724)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1724
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92261_length_1724_cov_3.168963"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(6..1163)
                     /locus_tag="PKH07_14685"
     CDS             complement(6..1163)
                     /locus_tag="PKH07_14685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36228.1"
                     /translation="MNRSVCTGVILTGLLLSAVHAHAQVGLDAEFAGFGARALGMGSA
                     FIALSDDATASEYNPAGLVILERPEVAIQTSYRLDRHRELQPVSFGNWLGQGARGAAP
                     RENEYDSYWIPSFASFTYPTKWAVVSICEYSPMYFKRSLTDSSGRYFIKRDWETQLTN
                     YGVSVGFAPFSKLYLGTTLKYGDFRYRQRDTMSSGTFLNSLEGNSLGANFGFIWKMHP
                     WFSLGGVYKTSQNLAGSYASTRVHLKIPRTVGMGVAFHPNDRLRILADVDQIEWSEFM
                     KERNTLEPNYDRDDVLRYHLGAEYLLKVFGEATPSPTALFVRCGAMREDSNRLYYHGT
                     FEPLRKAFPKEEPINHLSCGLGVARSRYQLDFAVDESSKGTQFILSTVLYF"
     gene            complement(1512..>1724)
                     /locus_tag="PKH07_14690"
     CDS             complement(1512..>1724)
                     /locus_tag="PKH07_14690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36229.1"
                     /translation="TDRGRIEALIKPPKRSPCGTIRLRLRHPEGKPIRSVTINGKPHD
                     NFDREGEWIILPKNAKDDLKIVASYH"
BASE COUNT          426 a          462 c          444 g          392 t
ORIGIN      
        1 ggttgctaga aatacaaaac cgttgagagg atgaactggg tgcctttcga actctcatcc
       61 acggcaaagt cgagttggta tcggctgcgg gcaacaccca gaccgcatga gagatggttg
      121 atgggctctt cttttggaaa cgccttccgc aagggttcga acgtaccatg atagtaaagc
      181 ctgttggagt cttcgcgcat cgctccacag cggacaaaga gcgcggtcgg cgatggcgtt
      241 gcctcaccga acactttgag caggtattcc gcgcccagat ggtatctcaa cacatcatct
      301 cggtcgtagt ttggctccaa tgtgttgcgc tctttcataa actctgacca ctcgatttga
      361 tctacatcgg caagaattcg aagccgatca ttgggatgaa atgcgacacc catcccaacc
      421 gttcggggaa tcttgagatg aacacgcgtg gaagcgtagc tgcctgcaag attctgagat
      481 gtcttgtaaa caccacccag cgaaaaccag gggtgcatct tccagatgaa tccaaaattg
      541 gcgcccaggg aattgccttc caatgagttc agaaacgtcc ctgacgacat cgtgtctctc
      601 tggcgatagc ggaaatcccc gtacttcagc gttgtgccaa gatacaactt cgaaaacgga
      661 gcaaatccca ccgacactcc gtagtttgtc aactgcgtct cccagtcgcg cttgatgaaa
      721 taccgtcccg aagaatcggt gaggcttcgc ttgaaataca tggggctgta ctcgcagatg
      781 gagacaaccg cccactttgt gggataggta aaactggcaa atgacggaat ccagtaagaa
      841 tcgtattcgt tttctctggg ggcagcgcct cgggcgccct ggccgagcca attgccaaaa
      901 ctcaccggct gcaattcccg gtgtctgtcc agccggtagg acgtttggat cgccacttcc
      961 ggtctttcga gaatcaccaa cccagcagga ttgtactcag atgcagtggc atcgtccgaa
     1021 agtgcgatga acgctgagcc catgcccagg gctcttgcac caaaaccggc aaactcagca
     1081 tccagaccaa cctgcgcgtg cgcgtggact gcagacaaca gcaagccggt cagaatcaca
     1141 cccgtgcaca ctgagcgatt cataccaatg ccccctgtgc tgtgctagac aacacacact
     1201 gctaaaaccc aagagtgctc atcgaacctc aagacatttt gtcgcaacaa gaatgttgct
     1261 ctagtctggc agaccagtca gagggatgtc cagacagagt gaattctagg gcggaaaaca
     1321 gtgcgcccgg tgactgctga atatccttcg gacacagttg cacctcacat tcagactaga
     1381 ctatgattaa tgatagcaca ttctgccaga gagacccaag agaaggcaga gtctgtgtag
     1441 ctgatctgat acactgcgtc ctctaagatg catgttcggg aaagaagagg tcttctctgg
     1501 cgtcaaatcc ctcaatggta agaagcgacg atcttcaggt catccttcgc attcttcgga
     1561 agaatgatcc attccccttc tctatcgaaa ttgtcgtgtg gtttgccgtt tatggtcacg
     1621 cttcggatcg gctttccttc agggtgacgc aggcgaagtc ggatggtgcc gcaggggctg
     1681 cgtttgggtg gcttgatcaa cgcttcgatg cgtcctctat cggt
//
LOCUS       DAOQUC010000978         1722 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_92462_length_1722_cov_2.863827, whole genome shotgun sequence.
ACCESSION   DAOQUC010000978 DAOQUC010000000
VERSION     DAOQUC010000978.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1722)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1722)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1722
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92462_length_1722_cov_2.863827"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1250
                     /locus_tag="PKH07_14695"
     CDS             <1..1250
                     /locus_tag="PKH07_14695"
                     /inference="COORDINATES: protein motif:HMM:NF024716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HNT36230.1"
                     /translation="IIRTREDTPSGLYRGVLEVSANRMKPRKMNLSVEVWDFAIPKEQ
                     HLLTVWGITEADYRRLYRERFDTRMAREMFDFLIDHRLSVTTLYSQQAAGEPKHGASV
                     GYPTLSDPAELKRLWDAGSRWWNLGYIHPVFADRAGKSIDDYVPDFIEMIEESLRVAD
                     AAGWPRSNMMIYFFDETHDFESLNRVATKVKTAFPDIPLMTTGYDRSYGVKGGPIDSS
                     MDIWCPLSPTYLEDLELIEKGRAIGKKAWWYVCCVPKGGNDLNFFCQFPAIRSRLLMG
                     TAAWKYKPDGFLYYRVAGWNHYEKPIETGPLTEWKPYFLPGPDGDGELICPGPNGALT
                     TLQFENIRDGIEDYEYYWVLQELIVRAQQGGLDVGDEVFLLDVPTDLLETITVYSEHP
                     KHLRQQRRKIAEAILKLKKMLDE"
     gene            complement(1337..>1722)
                     /locus_tag="PKH07_14700"
     CDS             complement(1337..>1722)
                     /locus_tag="PKH07_14700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36231.1"
                     /translation="ILSTTPPNSQKLTTGDTYSLSALASCLSNEDGLRLLWTLRNNGG
                     AFQTKPMEFLSQSSEWQEKVTTFSSVVIPNNGPVVLKAQIVNASPTPTVLAEDSVIFQ
                     IATPTRTPTPTPTLSTGVNVWTDYK"
BASE COUNT          398 a          438 c          467 g          419 t
ORIGIN      
        1 tcattatccg aacgcgtgaa gacacgccat cgggactcta ccgaggagtg ttggaggtga
       61 gtgccaatag gatgaagcct cggaagatga atctgtctgt tgaggtctgg gatttcgcca
      121 ttcccaaaga gcaacacctg ctcaccgtat gggggattac tgaggcggat tacaggagac
      181 tctacagaga acgctttgac acaagaatgg cgcgcgagat gtttgacttc ttgattgacc
      241 atcgcctttc tgtgacaacc ctgtattcac aacaagcagc gggagaaccg aagcatggtg
      301 catctgttgg ctatccaaca ctttcagatc ccgctgaatt gaaaaggctt tgggatgccg
      361 gctctcgttg gtggaatctt ggctacatcc atcccgtttt cgctgacagg gcaggcaaga
      421 gcattgatga ctatgtgcct gacttcatcg agatgatcga ggaatccctg cgcgtggcag
      481 atgcagcggg ttggcctcgt agtaacatga tgatctactt cttcgacgaa acgcatgatt
      541 tcgagtcgct gaaccgtgtt gccacaaagg tcaagacagc ctttcccgac attccgctca
      601 tgacaacggg ttacgaccgc agctacggtg tcaaaggggg tcccattgat agcagtatgg
      661 atatctggtg tcccttgagc ccgacctacc ttgaggattt ggagttgatc gaaaagggac
      721 gcgcgattgg caagaaggcg tggtggtatg tttgttgtgt accgaaaggg ggcaacgatc
      781 tgaacttctt ctgccagttc cccgccattc gttccaggct cctgatgggg acagcggcct
      841 ggaaatacaa gccggacggg tttctctatt accgggttgc cggatggaat cattatgaga
      901 aaccgataga aactggccct ctgaccgaat ggaaaccata ttttctgccg ggtcccgatg
      961 gtgacggaga gttgatatgc ccaggaccca acggcgcatt gaccactctt cagtttgaga
     1021 atatccgcga cggcattgag gactatgagt attattgggt acttcaggag ttgattgttc
     1081 gtgcccagca aggtggactc gacgttggtg atgaggtctt tctgcttgat gttccgacgg
     1141 atctgttgga gaccatcaca gtctactcgg aacacccgaa gcaccttcga caacagcgca
     1201 gaaagattgc cgaagcaatc ctgaaactga aaaagatgtt ggatgagtga agcatctctt
     1261 atccttatcc agagggattc ctctcccttt atcactccat ccagaatgac aggtcagctg
     1321 tgggatcagt cccacactat ttgtagtccg tccagacgtt cacccccgtc gaaagtgttg
     1381 gtgtcggtgt tggtgtccga gtcggcgtgg cgatctggaa tatcaccgaa tcctccgcca
     1441 aaactgtcgg cgtcgggctg gcgttcacga tctgcgcctt caacaccacc ggcccgttgt
     1501 tcgggatcac aacactgctg aacgtcgtga ccttctcctg ccattccgaa ctttgggaca
     1561 aaaactccat cggcttagtc tggaacgccc caccgttgtt tcgcaacgtc caaagcagcc
     1621 tcaacccgtc ttcgttcgac aggcacgatg ccaatgcaga aaggctatac gtgtcccccg
     1681 tcgtcagttt ctggctgtta ggtggcgtcg tcgagagaat gt
//
LOCUS       DAOQUC010000979         1721 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_92527_length_1721_cov_3.647059, whole genome shotgun sequence.
ACCESSION   DAOQUC010000979 DAOQUC010000000
VERSION     DAOQUC010000979.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1721
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92527_length_1721_cov_3.647059"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            128..>1721
                     /locus_tag="PKH07_14705"
     CDS             128..>1721
                     /locus_tag="PKH07_14705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36232.1"
                     /translation="MYKDPNELYQACGEDFLAAFGELVKTALPLNNYLEKEPPKQKSG
                     GLKRGRPTAAQMMVQFVQKRARLLRNEMKTPYASLEISGRREILPIRGTMFKEWLNVV
                     YFEETGKPPSKFSLEQAITTVAGLAALQGSTENIYLRVAREAARVLVDLNNDCGEVVE
                     ITPDGWQIITNPTVNFYRPSHSSTLPRPSQRKDLTLLRKYLNTSEEGFVLLSSFLMGT
                     INGLRPFPILELRGEQGTAKSTTARLIQKLLDPSKIDIRAEPKDIRDLMIAAEGGYLL
                     SYDNISYLHSWLSDALCRLSTGGGFGTRKLYTDSEEQLFSLARPVILNGIDDVINRGD
                     LMDRALIIDLSPIPTDKRKEEKILWEEFEQDIPSILGALYDAVAIGLKHLNSVSLKVL
                     PRMADFAKWIVACEPALPWQPGEFLRVYAENKDEAAVCLIESDDFTSAVRRLIADRKE
                     WKGTARELKKAIDADVTIDDYIKQSRAWPKNPQNVSHKMKLAAPSFRSMGIDVIFRTS
                     HGKKRLIVLTMSTKGETTVSALE"
BASE COUNT          488 a          385 c          463 g          385 t
ORIGIN      
        1 atcgttcgag aagcggatga agcagggggg caattcgtcc tggggatcca caagcgactc
       61 caggaaatcc actgggaagg ctctgccttc gtgatcgata tgcgtaatgc ctcgtggaga
      121 aagtagcatg tacaaagatc ccaatgaact gtaccaagcg tgtggcgaag attttctggc
      181 ggcatttgga gaattggtca aaacagcgct accgctaaac aattacttgg aaaaggaacc
      241 gccaaaacag aagtctgggg ggctgaagag ggggcgtccc actgcggcgc agatgatggt
      301 ccagtttgtg cagaaacgcg caagactact ccgcaatgaa atgaaaactc cttacgcgag
      361 cctggaaata tcaggcagaa gagaaattct cccgatcagg ggaacgatgt tcaaggagtg
      421 gcttaacgtc gtctacttcg aagaaactgg caagccaccg tccaaatttt cattagagca
      481 agcaatcacg actgttgcgg gtctggcagc gcttcagggc agcaccgaaa acatatattt
      541 gcgtgtggcg agggaagctg cacgtgttct ggtcgatctg aataatgact gtggagaagt
      601 ggtggagatt acaccggacg gatggcagat cattacgaat ccaactgtca acttttatcg
      661 gccaagccat tccagcaccc tcccacgacc atctcaacgt aaggatttga ccttgctccg
      721 gaaatatctc aacacgagcg aggaaggatt tgttctgttg agttcattcc taatggggac
      781 aatcaatggg ctgcgaccat ttccgattct ggaactgaga ggtgaacagg gcacagccaa
      841 gagcaccacc gccaggctaa tccaaaaact gctcgatccc tcaaaaattg atatcagagc
      901 tgaacccaaa gacatccggg atctgatgat cgcggccgaa ggtggatatt tgctttccta
      961 cgacaacatt tcatatctac attcttggtt gtctgatgct ctgtgtcgac tgagcacagg
     1021 agggggattt ggaacacgaa aactgtatac cgacagtgag gaacaactgt tctccctggc
     1081 acggccggtg atactaaatg gaattgatga tgtcatcaac cgaggggact tgatggatcg
     1141 ggcgctcatt atcgaccttt ctccaattcc aacagacaaa cgaaaggagg agaagatact
     1201 ctgggaagaa tttgaacaag atattccttc catcctgggt gctctgtatg atgcggtcgc
     1261 aattggtttg aaacacttga acagtgtgag tctgaaggta ctaccccgaa tggccgattt
     1321 tgccaaatgg atcgtggctt gtgaaccggc gcttccgtgg cagccaggag aatttcttcg
     1381 tgtttatgct gaaaacaagg atgaggcggc cgtttgccta atagagagtg atgactttac
     1441 gtcagctgtt cgacggctca ttgctgatag gaaagaatgg aaaggtacgg ctcgggagtt
     1501 gaaaaaagct atcgacgccg atgtaacaat agatgactat atcaagcaat caagggcttg
     1561 gccaaaaaac cctcagaacg tatctcacaa aatgaagctc gcagccccga gcttcagatc
     1621 gatggggatc gatgtcattt tccggaccag ccatggaaag aagagactaa tcgtcttgac
     1681 tatgtcgacg aagggggaga caacggtcag tgctttggaa g
//
LOCUS       DAOQUC010000980         1720 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_92645_length_1720_cov_2.608408, whole genome shotgun sequence.
ACCESSION   DAOQUC010000980 DAOQUC010000000
VERSION     DAOQUC010000980.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1720)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1720)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1720
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92645_length_1720_cov_2.608408"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..715
                     /locus_tag="PKH07_14710"
     CDS             <1..715
                     /locus_tag="PKH07_14710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36233.1"
                     /translation="GITWPRNHVYVYYMDERRYVDLGRIGDINPQAIFLDKNGTAYTT
                     DDYGYIIRCDPDTNELRRLNVKCPHLAYRIGWHNVPYDVVPSPDGETFFGIDWGFENH
                     VWEFDPRGEGSMRSFGRVWAPPEWNNDHSLEYYQLRALVFGADGKLYFTGRVGWEQDQ
                     VQHLIRLDTVRGKTEDLGGIDFPGRRGFHAASATFDFYGNLYIAEAGLHPTGMYIYRP
                     DYVSKGKRLFSWKDVKMWG"
     gene            805..>1720
                     /locus_tag="PKH07_14715"
     CDS             805..>1720
                     /locus_tag="PKH07_14715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36234.1"
                     /translation="MTKDLSDGLRQRTWPKPQEVLTGNYVQASYVWEGRVFNRALRMH
                     QHVSEPITPGESAITSLCRGLNGKIYGATSGKRSHLFYYDPGPRGDGVCDLGVMEGVT
                     AIRRSLVAIAPDLIIAGASEVNDPSQGGALYRHATAHDHMDEYGTSKGKVEKILVPVE
                     GECVAALAADGLRQMVYGLSTPSGIFFRYDVKAGTVKQYGQVASDKVFSHTLVLDAKG
                     NVYGASSLGELFRYCPEQDSVASLCKRIPSLAGREFYNRLDAAVLEPRSGLIYGAGSA
                     DGVLFVLDPESLEIRSLGKATAEPRVRAM"
BASE COUNT          397 a          429 c          510 g          384 t
ORIGIN      
        1 cgggattaca tggccgagaa accatgtcta cgtctactac atggatgaaa ggcgctacgt
       61 tgacctcgga cgcatcggtg acatcaaccc gcaggcgatt tttctggaca agaatggcac
      121 ggcctacacg accgatgatt acggctacat catccgttgc gaccccgaca ccaacgaact
      181 gcgacggctc aatgtgaagt gtcctcatct ggcgtaccga atcggttggc acaatgttcc
      241 ttatgacgta gttccttcgc cggacggtga gactttcttc ggaatagatt ggggttttga
      301 aaaccatgtt tgggagtttg atcctcgtgg cgagggaagt atgcgcagct tcggtcgggt
      361 ctgggcgcca cccgaatgga acaacgatca ctcgcttgaa tactaccagc tcagggcttt
      421 ggtttttggg gcggacggta aattgtattt cactggcaga gtcggatggg aacaagacca
      481 agttcagcat ctcatccgcc tggacaccgt gaggggcaag accgaggatc tgggtggcat
      541 tgattttcca ggacggcgag gctttcatgc cgccagcgcc acgttcgact tctatgggaa
      601 tctctacatt gccgaggcag gactgcaccc gacagggatg tacatctacc gacccgatta
      661 cgtctccaag ggcaaacggt tgttctcctg gaaagacgtg aagatgtggg gataggcttt
      721 catgaagaaa ctggtgttca ccatgaaaaa gcgactgcag ttatacacag aaccagtgag
      781 tcagccagaa aggaatcagc aacgatgacg aaagatttat cggatgggct ccgacaacga
      841 acttggccaa agccccagga agttctcaca ggaaactatg ttcaggcttc ctatgtctgg
      901 gaaggaaggg ttttcaacag ggctctgcga atgcaccagc acgtctcgga gccgattaca
      961 ccaggtgaat ccgcaatcac ctcgctgtgt cgaggtctca atggcaaaat ctatggagcc
     1021 acttcaggca agagaagcca tctgttctac tacgatcccg gccctcgtgg tgacggtgtt
     1081 tgtgatttgg gtgtgatgga aggcgtcacg gcgattcggc gttcgctggt agcgattgca
     1141 cccgacctga tcatcgcggg tgcctcggaa gtcaatgatc cttcccaagg gggtgccttg
     1201 taccgacatg caacggcaca cgaccacatg gatgagtacg gaacctcgaa aggaaaagtg
     1261 gagaagatac tcgttccggt ggaaggtgaa tgcgttgcgg cgcttgctgc ggatggactt
     1321 cgtcagatgg tctacgggtt gagcacgcct tcaggcatct tcttccgata cgatgtaaag
     1381 gcgggaaccg tgaagcagta tgggcaagtg gccagtgaca aagtgttctc gcacacactg
     1441 gtgttggacg cgaaaggcaa cgtctatgga gcctcttcgc ttggggaact gttccgatac
     1501 tgtcccgaac aggatagcgt ggcatcgctt tgcaagcgca ttcccagcct tgcggggcgc
     1561 gagttctaca atcgccttga tgcagcggtg ttggagcctc ggtcgggttt gatctacgga
     1621 gcgggcagtg ccgatggagt tctctttgtt ctggatccgg aatccctgga gattcgctcg
     1681 ctcggcaaag ccaccgcaga gcctcgggtg cgggcaatgg
//
LOCUS       DAOQUC010000981         1719 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_92694_length_1719_cov_3.578125, whole genome shotgun sequence.
ACCESSION   DAOQUC010000981 DAOQUC010000000
VERSION     DAOQUC010000981.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1719
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92694_length_1719_cov_3.578125"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..959
                     /locus_tag="PKH07_14720"
     CDS             <1..959
                     /locus_tag="PKH07_14720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36235.1"
                     /translation="VQNLIFQTNLLAGECGRQCVQFFSRLERELYLTALIAEKPSTDS
                     SEIRRRCDGIQKEFQLRDVLLIQPSGTIQYTANSPEAIGQPVEGTLFDNTRLAEVWRE
                     AMRTGRSLTDFDPSSSTSTGYLAVRGDWENAAVAVAEYNALTFDVALRQCATVQELSD
                     AGDSGIHVSLETREGGTYLSLSSALQAANGQSIVEASESVNLGGVQMILRVRADSSAS
                     QALVRSRKILILATAVLIFAACVLAWAMLRSLGMISTGTVQLSPKVQRVQDIAIAASA
                     LDPEQHPEVLAFIRQLVRDSVRQADGEKDDPRPKQQNQPEIR"
     gene            complement(963..>1719)
                     /locus_tag="PKH07_14725"
     CDS             complement(963..>1719)
                     /locus_tag="PKH07_14725"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNT36236.1"
                     /translation="HRGPITSWEEFEKYPWPDPAKAGTTIAEWFCENLPKDMGIILCG
                     GFAHFCEYVTWLMGYESLCYALHDQRDLVRAISDRLTEIFTVVAERMLKFDRIQVVWG
                     SDDMGFRGGTLISPADLREFVFPGHKKMAEMSHDAGRPYLLHACGNLSEIMDDLIDDV
                     KIDAKHSFEDTIEQVTDAKRLYGNRIALLGGIDVDFLCRATEEQVRERVRKTLEVCMP
                     GGGYFLGTGNSVANYIPVDNFLAMLDEGRRFLG"
BASE COUNT          399 a          466 c          452 g          402 t
ORIGIN      
        1 acgtccagaa cctaatcttt cagacaaact tgcttgccgg tgagtgcggg cggcagtgtg
       61 tccagttttt cagcagatta gagcgagaat tgtatctgac agccctgatt gctgaaaagc
      121 cttcaacaga ttcctcagag attcggagac gctgtgacgg catccagaag gaattccagc
      181 tgagggatgt gctcctgatc caaccgtcag gaacgattca atacacggcc aattcgcccg
      241 aggctattgg tcagcctgta gaaggaacgc tttttgacaa tacgcgactt gcggaagtct
      301 ggcgagaggc aatgagaacg gggcgcagcc tgacggattt cgatccctct tcatcaacgt
      361 ccaccggtta tctcgccgtc cgcggtgatt gggaaaatgc agcggtagcc gttgcagagt
      421 acaacgcgct gacatttgat gtggcactgc gccaatgtgc aactgttcag gagttgtctg
      481 atgccggcga cagtggaatt catgtttctt tggaaacacg agaagggggg acgtatctca
      541 gtctttcgtc cgccctgcaa gccgcgaacg gtcagagcat tgtcgaggcg tccgaatcgg
      601 tgaaccttgg cggtgtgcag atgattcttc gcgtacgggc tgattcgagt gcaagtcagg
      661 ctcttgtaag aagcaggaag attctgatcc ttgccacggc agttttgatt ttcgctgcgt
      721 gcgttctagc gtgggcaatg cttcgaagcc tggggatgat ctcaacagga acggtacaac
      781 tctctcccaa ggttcaacgt gttcaggata tcgccattgc agcctcggca ttggatcccg
      841 agcagcatcc agaagttctt gccttcatca gacagttggt tcgagactcc gttcgccaag
      901 ccgacggtga gaaagacgac ccccgcccca aacaacagaa tcagccagaa attagatgag
      961 gcctacccca ggaatctcct gccctcatcc aacatcgcca aaaagttgtc tacggggata
     1021 tagttggcga cgctgttgcc agttcccaga aagtagccac cacctggcat gcagacttcc
     1081 agtgtcttac gaacccgctc gcgcacctgc tcttctgtag cgcggcagag gaaatctacg
     1141 tctataccac ccaacagggc gatccgattg ccataaagcc tcttggcgtc ggtgacctgt
     1201 tcgattgtgt cttcgaaaga gtgttttgcg tctatcttaa catcgtcaat gagatcatcc
     1261 ataatctcac tcaggttgcc gcaggcgtga agcagatagg gtcttccggc atcatgtgac
     1321 atctcagcca ttttcttatg tcccggaaag acaaactccc gaaggtcggc agggctgatc
     1381 aacgtccctc ctcgaaatcc catgtcgtcg ctaccccaga cgacctggat tcggtcgaat
     1441 ttcagcatgc gttctgccac aactgtgaat atctccgtca gtcggtcgct gatggctctg
     1501 acaagatcgc gttgatcgtg aagtgcatag cagagggatt catagcccat caaccaggtc
     1561 acatattcgc aaaagtgcgc aaagccgccg cagagaatga tgcccatatc ctttgggagg
     1621 ttttcacaga accactccgc gatggtcgtt cccgctttcg cgggatctgg ccacgggtac
     1681 ttctcaaact cctcccaact ggtgatcggg cctcgatgc
//
LOCUS       DAOQUC010000982         1717 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_92893_length_1717_cov_2.930806, whole genome shotgun sequence.
ACCESSION   DAOQUC010000982 DAOQUC010000000
VERSION     DAOQUC010000982.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92893_length_1717_cov_2.930806"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1088)
                     /locus_tag="PKH07_14730"
     CDS             complement(<1..1088)
                     /locus_tag="PKH07_14730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36237.1"
                     /translation="MDRERPFVVKCPVGGEEYPSNDFAAFRRSGMKDRSLLTGEYADD
                     GRGWMGPNGVKYWFVGYACHWMWGKHWKPAVVQLSRSHLLTGDPKYARKALIMLDRIA
                     ECYPDFEYCSQSRYGELKGGKYHGKILNSIWETGTLDSLAIAYDGVFEALLGDDAVNL
                     NHRDAKAIRANIEANLLEEGLDSVENGRIRGNWGMHQRASLLAALVRQNGPTGEIIKK
                     IMEYPGTPQNAWQAGVNYALYNLIFADGMPFETAPGYCFTWVSNLCIIADALQLASVN
                     LFENPKVLRTLDAHLDLICLGQFMPSVGDSGSIHAGWSGMNATPFLRAYRATRNPRYA
                     WAATQIDSKIFSADYRSFDDLFLEPIAA"
     gene            complement(1061..1501)
                     /locus_tag="PKH07_14735"
     CDS             complement(1061..1501)
                     /locus_tag="PKH07_14735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36238.1"
                     /translation="MHFQTLVLLSALILMGFIGFRTWPVFADDSSETGLVESTSTLYP
                     PARFRTPQWPLKTNRTLLTQEQIDRARKACADNPDAAKIRDEILKETEYWISKSDEEL
                     REMLPGAEVPRAFNESSEGCPVHGKEIYKHGTYPWIWTENARLL"
     gene            complement(1536..>1717)
                     /locus_tag="PKH07_14740"
     CDS             complement(1536..>1717)
                     /locus_tag="PKH07_14740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36239.1"
                     /translation="HGARRGQWSHAIVPPSAQLPSLFQPTPNRFKHVERILTDARIDN
                     NPLASEILDASTYSR"
BASE COUNT          389 a          445 c          466 g          417 t
ORIGIN      
        1 tccgcagcaa ttggttccag gaacaggtcg tcgaaggagc gatagtcggc gctgaagatt
       61 ttagagtcta tctgcgttgc cgcccaggcg tatcttggat tcctggttgc gcgatatgct
      121 cgcaggaatg gagttgcgtt cattccgctc cagcctgcgt gaatgctccc ggagtcgccc
      181 actgatggca tgaactgccc cagacagatg agatccaaat gtgcatcgag tgtccgcagg
      241 actttgggat tctcaaaaag attcacggaa gcgagttgga gcgcatccgc aatgatgcac
      301 agattactca cccaggtgaa acagtagccg ggagctgtct cgaagggcat tccgtccgca
      361 aaaatcagat tgtagagagc atagttcacc cctgcctgcc aggcgttttg gggggtgccg
      421 ggatactcca ttatcttctt gatgatttcg ccagttggac cattctgccg aaccagggct
      481 gccagaagtg atgctcgctg gtgcattccc cagttgcctc gaatccggcc attttcaaca
      541 gagtccagtc cctcctcaag caagttggct tcgatattgg ctcgaatcgc ttttgcatca
      601 cgatggttca gattgactgc atcatcgccg agcagtgcct caaaaacacc gtcataggcg
      661 atggcaaggc tgtctagcgt tcccgtctcc caaatgctgt tgagaatctt tccgtgatac
      721 ttgccgccct taagttctcc atatctgctt tgggaacagt actcgaaatc gggataacat
      781 tctgcaatgc ggtcaagcat gatgagagcc ttgcgggcat atttcggatc accggttaac
      841 agatgagaac gcgataattg gacgacggca ggtttccaat gcttacccca catccagtgg
      901 caggcatagc caacaaacca gtacttcact ccgttgggtc ccatccagcc tctgccgtca
      961 tcggcgtatt cgcctgtcag caggctgcgg tccttcatac cggagcgacg aaacgccgca
     1021 aaatcgttgc ttggatattc ctcgccacca acaggacatt tcacaacaaa cgggcgttct
     1081 cggtccatat ccatggatag gtgccgtgtt tgtaaatctc cttgccgtgc acagggcagc
     1141 cttctgaaga ttcattgaat gcgcgtggca cctcagcgcc tggaagcatc tcgcggagtt
     1201 cctcatcaga ctttgaaatc caatactccg tctctttgag aatttcgtca cgaatctttg
     1261 cggcgtcggg attgtctgcg caagccttgc gggcacggtc aatctgctcc tgtgtgagaa
     1321 gtgttcggtt ggttttcaga ggccattggg gcgtccggaa acgagccggt ggatacaagg
     1381 ttgatgttga ttccacgagg cctgtctccg aggaatcgtc ggcaaaaacg ggccatgtcc
     1441 tgaaaccaat gaatcccatg agtatcaggg cagatagcag gacaagggtc tgaaaatgca
     1501 tgtcgctctc cattcgacga tcagatgacc agaggctacc ttgagtaagt ggatgcgtca
     1561 aggatttcgg aagccaaggg attgttgtca atgcgagcat ctgttagaat cctctcgaca
     1621 tgtttgaatc tgtttggagt cggttggaac agagaaggga gttgagccga tggcggaaca
     1681 atggcgtgcg accactgtcc tctgcgtgcg ccgtggc
//
LOCUS       DAOQUC010000983         1717 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_92922_length_1717_cov_2.218412, whole genome shotgun sequence.
ACCESSION   DAOQUC010000983 DAOQUC010000000
VERSION     DAOQUC010000983.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92922_length_1717_cov_2.218412"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="PKH07_14745"
     CDS             <1..249
                     /locus_tag="PKH07_14745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36240.1"
                     /translation="VVLRQAVGFGVPIDSVVSRRGKDVIFIAEQNRARMVPVELGLRT
                     ERMVQVISDILSPGMQVVIQGQKFCEEGTLLRVVQEAK"
     gene            250..>1717
                     /locus_tag="PKH07_14750"
     CDS             250..>1717
                     /locus_tag="PKH07_14750"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNT36241.1"
                     /translation="MFLSDASVKRPVAMSCLFIALTILGTNAWRKMSLELMPSFSIPF
                     ITIVTIYPGASPEEIEVDVAKRIEDAVGKISGLKNITSTCMSNVHQALLEFAMGKNVD
                     IVATEVSRQLDTILPDLPVNAEKPQVQTFDVNSTAVVTFALTGDVPLDELYDYADNEM
                     SDRLTVIPGVAKVELTGGAEREVHILLDRDAVAARGLTSMDIVRTVQNGLGKTPAGNI
                     RGGGVEYTVKYDADFRAVEELGSLEIVNDAGRRCYLRDVATVSMSTEELRKTARLDGR
                     QAVAVKVYKREDANAVRVVDQVRVVLDKMRQTMPGGMELVWVTDDATFIRASNSSAWV
                     NVAQGIVLTAAVLFFFLYDLRALLVVSISMPLTVVIGLFFIQAAGFSLNMATLIAIGM
                     SVGILVTNSIVVMEAIFKCLHDNHSPREASIIGASDSFIPVLASAGTNLVVLFPLAMM
                     KSKVAEFIRPLALTMLIMTLVSLFISFTLTPMLCSLLLR"
BASE COUNT          354 a          419 c          511 g          433 t
ORIGIN      
        1 gtggtgcttc ggcaggcagt cggttttggg gttccgattg acagtgttgt cagccgcaga
       61 ggcaaggatg tcattttcat cgcggaacaa aacagagcgc gaatggtacc cgtcgaactt
      121 gggctgagga cggagagaat ggtgcaggtc atttcggaca tcctttcacc cgggatgcag
      181 gttgtgatcc aagggcagaa attctgtgag gaaggcacgt tgctccgagt cgttcaggag
      241 gccaagtaga tgtttctttc ggacgcttca gtcaagagac ccgtggcaat gagttgcctg
      301 ttcattgccc tgacgatctt gggaaccaac gcctggcgca agatgagtct ggaactgatg
      361 cccagcttca gcattccatt catcaccatc gtaacgatct atccaggcgc aagccccgag
      421 gagatagagg ttgatgttgc caagcgcatt gaggatgccg ttggaaagat aagcggtctg
      481 aagaacatca ccagcacctg catgtcaaat gttcatcagg cgttgctgga gttcgcgatg
      541 ggtaagaatg tggacattgt ggcgactgag gtcagtcggc aactggacac catactgccc
      601 gatctccctg tcaacgcgga gaagccccag gtacagacgt ttgatgtgaa ctccacggca
      661 gtggtcacgt tcgctctgac aggcgatgtt ccactcgacg aactctacga ctatgcggac
      721 aacgaaatga gcgatcggct taccgtcatt ccaggtgtgg cgaaagtcga attgaccggt
      781 ggggctgaga gggaagttca tattctgctg gatcgggatg ccgtggcggc tcgcggtctg
      841 acaagcatgg atattgtgcg aactgtgcag aatggccttg ggaagacgcc cgccggcaac
      901 attcgaggcg gcggggttga gtacacagtc aagtatgacg ccgacttccg agctgtagag
      961 gaactgggca gtctggagat tgtcaacgat gccggtcgtc gctgctactt gcgggatgtt
     1021 gcgacggtca gtatgtccac tgaggaactc cggaagacag cacgtctcga tgggcgtcag
     1081 gcagttgccg tgaaggtcta caaacgagaa gacgccaatg ctgttcgggt ggttgatcag
     1141 gttcgtgtcg tcttggacaa gatgcgtcag acgatgccgg gtgggatgga attggtttgg
     1201 gtgacggacg acgccacgtt cattcgcgct tccaacagca gtgcctgggt taatgttgct
     1261 caaggcatcg ttctcacggc cgccgtgctg ttctttttcc tttacgatct gcgggcgttg
     1321 cttgtggtat cgatcagcat gcctttgact gtggtgattg gcttgttctt catccaagcg
     1381 gcgggcttct cgctgaatat ggcgacactg attgccattg gcatgtctgt tggcattctg
     1441 gtgaccaatt cgatcgttgt catggaggcc atattcaagt gtctccatga caaccattct
     1501 cctcgggagg cttccatcat cggtgcctcc gattcattta tccccgtgct tgccagtgcc
     1561 ggcactaact tggttgttct gttcccactg gcgatgatga agtcaaaagt cgctgagttt
     1621 attcgtccgc ttgccttgac gatgctcatc atgaccctgg tgtctttgtt catctccttc
     1681 acgctcactc cgatgttgtg ttcgctgctt cttagac
//
LOCUS       DAOQUC010000984         1716 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_93026_length_1716_cov_2.267309, whole genome shotgun sequence.
ACCESSION   DAOQUC010000984 DAOQUC010000000
VERSION     DAOQUC010000984.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1716
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93026_length_1716_cov_2.267309"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            20..>1716
                     /locus_tag="PKH07_14755"
     CDS             20..>1716
                     /locus_tag="PKH07_14755"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HNT36242.1"
                     /translation="MNRCELHIQGASEGSTKLLLEPIRPNGIICPVSPIEGISGSIVH
                     IDVHSSDPFGSKNVEGAIVVLDYNCWDNWLRAFRLGAKAVIFVPCGQTYSWHSHFSYA
                     NANLPRFYYAGSAADLPDGAEANIHSEVVWEDKVGQNVLAFFKGTDPVFHQDKEEVIV
                     VATSVDSYGEVPTLSPGARAAANCAGLLRLAEQISENRPKRHVLIAFFDGQSRGHLGV
                     SAFYKALEKEQKEVTVEKRKEYLENEWAFLLDLEKALASERPLQSGTSVRRELVRRLK
                     EAAKSHSFRIGGELYDLRKQLASLQEDSTRHQEVVSEIEERQAESNLWNDLRRTLARD
                     SITSTVSNELGIALADVRRDMLIRKAELEFERTALSIDEAIKELLSEMWISMHLSFLF
                     GDTTTRYGLIIGGDSSLCSGDDLPGLYTKIQSTFLNAYNTVQEKNGSVRLFEATSADG
                     TLDPPRLLWAAPFLIHSGEVAGKAGIYNLCVGTVQEHLPREGTPQDTLDRLDVDRLEN
                     QVKDFSNLFIQLASEEGLSLRRSIKTQIFYSVSKFESDNRPTGPTVMARAHGNAMPN"
BASE COUNT          414 a          444 c          453 g          405 t
ORIGIN      
        1 cccggttgct caaacccgtg tgaatcgttg tgaacttcac attcagggtg cttcagaggg
       61 ctcaactaag ttactcctgg aaccgatacg tcccaacggt atcatttgtc cagtctcccc
      121 gatcgaaggc atatcgggtt ccatcgtgca tattgacgtt cattcttcag atccgtttgg
      181 aagcaaaaat gtggaaggag ccatagtcgt cctggactac aattgttggg acaactggct
      241 tcgcgcattc aggctcggag ccaaggcggt catctttgtt ccctgtggtc agacttattc
      301 ctggcattca cacttcagct acgcaaacgc aaatctgccg cgcttctact atgcgggaag
      361 tgcggctgac cttccggacg gtgccgaggc gaacatccac agcgaggtgg tctgggaaga
      421 caaggtcggt cagaacgtac tggctttttt caagggcacc gatcctgtgt tccatcagga
      481 taaagaggaa gtgattgtcg tagccacttc cgtggacagt tacggtgagg tgcctacgct
      541 ttcaccaggt gccagggcgg ctgccaactg tgcaggactc ctcagactcg ccgaacaaat
      601 cagcgagaat cgtccgaaga ggcacgtatt gattgctttc tttgacggtc agtctcgcgg
      661 acatcttggc gtttcagctt tctataaggc tctcgagaag gaacagaagg aagtcacggt
      721 cgagaaacga aaagagtatc tcgaaaatga atgggcgttt ctcttggatc tggagaaggc
      781 gttggcgtcc gaacgtccac ttcagtcagg aacatcggtc aggcgcgaac tggttcgacg
      841 actcaaagaa gcagcaaagt cccattcgtt ccgtatcggc ggcgaactct atgatcttcg
      901 aaagcagttg gcgtccctgc aagaggactc cacccgtcac caggaagtcg tcagcgaaat
      961 cgaagaaagg caagcggagt caaatctgtg gaatgatctt cgtcgaacgc ttgctcgcga
     1021 cagcatcacc tcgacagtct ccaacgaact tggtattgca ctggccgacg tacgaaggga
     1081 tatgctcatc cgaaaggcgg aactggaatt tgagagaacc gctctttcga tcgatgaagc
     1141 gatcaaggaa ctcctctccg agatgtggat ttccatgcac ttgtcgtttc tgttcggtga
     1201 cacgaccaca cgatacggct tgatcattgg tggtgattcc tctctgtgtt ccggtgatga
     1261 tctccctgga ctctacacta agattcagag cactttcctg aatgcctaca acactgttca
     1321 ggaaaagaat ggttcggtgc gactttttga ggctacttcg gcggatggaa ccttagatcc
     1381 accaaggctt ctttgggcgg ctccttttct catccacagc ggcgaagtgg ctgggaaggc
     1441 gggcatctac aatctctgtg ttggtacagt tcaggagcat ctgcctcgtg agggcacacc
     1501 tcaggacacc ttggacaggc ttgatgtaga ccgcttggaa aaccaagtca aagacttcag
     1561 caacctgttc atccaactgg catccgaaga gggcttgtca ctcaggagat ccatcaagac
     1621 acagattttc tactcagtct ccaagttcga atcggacaat cgcccgactg gaccaaccgt
     1681 catggcgcga gcacatggca acgccatgcc gaatat
//
LOCUS       DAOQUC010000985         1711 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_93413_length_1711_cov_3.910628, whole genome shotgun sequence.
ACCESSION   DAOQUC010000985 DAOQUC010000000
VERSION     DAOQUC010000985.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1711)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1711)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1711
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93413_length_1711_cov_3.910628"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1414
                     /locus_tag="PKH07_14760"
     CDS             <1..1414
                     /locus_tag="PKH07_14760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit A family protein"
                     /protein_id="HNT36243.1"
                     /translation="TLDCAACILTPKMSSVRAHPNITLWTYSQVESVSGYAGNFKATV
                     RRKARYVIEKDCVGCLECINACVFKEPKFDNDFDLGLSKRKPVYMPFPQATPSVVLID
                     PESCLYLSKGKCKQSCMAACERNAIDFKQQDVLEEVEIGAVIIATGFKTFDARRVPVY
                     GYGKYPNVFTSLEVERLVNASGPTQGELVMRNGRKPESVAIIHCVGSRDRDTNAYCSK
                     VCCMYSLKLAHLIKERTEANVYNFYIDMRTPGKGYEEFYNRLLEEGVQFIRGRAAEVT
                     DATMTLAEQGKLIVRVEDTLISVVRRIPVDMVVLSVGLEPQADAEEIRRLFNISCSHE
                     GWFLERHPKLAPVSTFTDGIFLAGACQGPKDIPDSVAQAGAAAAEALAMIDKGFVEME
                     PNTAWIDESLCSGCKTCSSMCPFHAITFDSVRGISVINEALCKGCGTCVAACPCGAAQ
                     QRLFTDNEIYQEIQGVLNYV"
     gene            1407..>1711
                     /locus_tag="PKH07_14765"
     CDS             1407..>1711
                     /locus_tag="PKH07_14765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HNT36244.1"
                     /translation="MSEPNFQPRIVAFFCNWCTYTAADLAGIARMSYAANVRVVRIMC
                     SGRLDPQFVLAAFQQGADGVLIGGCHPGDCHYQEGNYKALRRVKLLKRVLREFGIAE"
BASE COUNT          384 a          463 c          471 g          393 t
ORIGIN      
        1 gacccttgac tgcgctgcgt gcatcctgac tccgaagatg tcttcggttc gggcgcatcc
       61 caacatcacc ctctggacat attcacaggt ggaatcggtc agcggctacg ctggcaactt
      121 caaagccacc gtccgacgca aagcacgtta cgtcatcgaa aaggactgcg tcggatgtct
      181 ggaatgtatc aatgcctgcg tcttcaagga accgaaattc gacaacgatt tcgatctcgg
      241 tttgagcaag agaaagcctg tctatatgcc ttttccacag gcaaccccct cggttgtttt
      301 gatcgatcca gagtcctgcc tctatctctc caagggcaag tgcaaacaaa gttgtatggc
      361 tgcctgtgaa agaaatgcca ttgacttcaa gcagcaggac gtcctggagg aagttgaaat
      421 cggggcggtc atcatcgcca caggcttcaa gacgttcgat gcccgccgcg ttccagtcta
      481 cggatacggc aaatatccga atgttttcac cagccttgaa gtcgagcgac tcgtcaacgc
      541 ttcgggaccc actcagggag aactggtcat gcgcaacggt cgcaaaccgg aatccgtggc
      601 gatcatccat tgcgttggct ccagagaccg cgacaccaac gcctactgct ccaaagtctg
      661 ttgtatgtat tcgctgaaat tggcacactt gatcaaggaa cgtaccgaag ccaatgtgta
      721 caacttctac attgacatga gaacgcctgg taagggttac gaggagttct acaaccgact
      781 tctcgaagaa ggtgtccagt tcatccgagg tcgcgctgca gaagtcacgg atgcaacaat
      841 gactctggca gagcagggta agttgatcgt tcgtgtggaa gacacgttaa tcagtgtggt
      901 tcgccgcatc cccgtggaca tggtagtgct ctctgttggt ctggagcctc aagccgatgc
      961 ggaggaaatc cgacggcttt tcaatatctc gtgcagccac gaaggatggt tccttgagcg
     1021 tcatccgaaa ctggctcccg tgtctacctt caccgacggc atctttcttg ccggcgcgtg
     1081 tcaaggtccc aaggacattc cggacagcgt ggcacaagcg ggtgccgcag cggcggaagc
     1141 gctggcaatg attgacaagg gctttgtaga aatggaaccg aatacagcgt ggattgacga
     1201 aagcctgtgc tccggctgca agacctgttc ttccatgtgt ccgttccacg ccatcacctt
     1261 tgactcggta cggggcatct ctgtgatcaa tgaagcgctc tgcaagggtt gtggcacgtg
     1321 tgtggctgcc tgtccttgtg gcgcagccca gcagcgactg tttaccgaca acgagatcta
     1381 tcaggagatt cagggagtcc tgaactatgt ctgaaccaaa cttccaaccg cgaattgtgg
     1441 cttttttctg caactggtgt acctacaccg cggcggactt ggcgggcatc gcccgaatgt
     1501 cttacgccgc caacgtccga gtggttcgga tcatgtgctc cggtcgtctg gatccccaat
     1561 ttgtgctggc agcatttcag cagggagcgg acggcgttct gatcggggga tgtcatcctg
     1621 gcgactgtca ctatcaggaa ggcaattaca aggcgctgcg gcgtgtcaaa cttctcaagc
     1681 gtgtcctgcg cgagttcggc atcgctgaag a
//
LOCUS       DAOQUC010000986         1707 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_93820_length_1707_cov_2.404964, whole genome shotgun sequence.
ACCESSION   DAOQUC010000986 DAOQUC010000000
VERSION     DAOQUC010000986.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1707)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1707)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1707
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93820_length_1707_cov_2.404964"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..451
                     /locus_tag="PKH07_14770"
     CDS             <1..451
                     /locus_tag="PKH07_14770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013042101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNT36245.1"
                     /translation="DWGQQRGLQMGPRLWREFIYPELKRMYRVVRDAGKYVFIHSCGD
                     VDELFVDLIEIGLNCFNPFQPEVMDVPGLLEHYRGRLTFFGGLSTQRTLPYGTPADVR
                     NETRRLIALGKDGGYILSPAHAVEGDVPLDNMLAFMEEAFQQPACVL"
     gene            527..>1707
                     /locus_tag="PKH07_14775"
     CDS             527..>1707
                     /locus_tag="PKH07_14775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005542918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNT36246.1"
                     /translation="MSEVRIGIIGASGRGGMAKHWHNPGGDSIVVGAADVSEGALVWF
                     KKNVNENGFVTKDHRELLDRKDIDAIAVMSPDYTHEQYVCDAFDAGKHVFTEKPMAIT
                     TEGCDRMLRAWVASGKQFMIGFNMRYMNIFRVMKDAIDSGSIGEVKAVWVRHFVAHGG
                     RWYYHDWHGTSRNTTGLLLQKASHDLDMIHWITGRYTTRVAASGGLDYYGGDKPNDLH
                     CIACPEKDTCVEYDHVNAASDNKMDLCVFRKEVDVEDNSIVMMELEGGIHASYMQCHF
                     TPDYCRNYTFIGTEGRLENLDDGSKVIIRMRDRSKRWRNLSHYDLDVKPASGGHGGAD
                     PLVCRDFVEMLLHGKRPLATPLAGRMSVATGCAATWSLRNGWQPVTIPPVPEDIREKV
                     Y"
BASE COUNT          412 a          445 c          486 g          364 t
ORIGIN      
        1 tgactgggga caacagcgcg gactgcagat gggtccgaga ctctggcgtg agttcattta
       61 tcctgagctg aaacggatgt accgagtggt tcgggacgct ggcaagtatg tgttcatcca
      121 ctcctgcggt gacgtagatg aattatttgt agacctcatt gaaatcggac tcaactgttt
      181 caatccattt cagcctgaag taatggacgt ccccggactc cttgagcact acaggggtcg
      241 cctcacattc ttcggcggac tatccaccca gcgaacgctg ccgtacggaa cgcctgcgga
      301 cgtaaggaac gaaacccgca gacttatcgc tttggggaaa gatggaggat acatattgtc
      361 gccagcccat gctgttgagg gagatgtccc tctggacaat atgctcgcgt tcatggaaga
      421 ggcgtttcag caaccagcat gcgttttgtg atttccgtgt tgcagcgatc tgcccgttgt
      481 cattattgta cccaagacgg atcattcaaa caggagcact attactatga gtgaagtacg
      541 gattggcatc attggcgcat caggtcgagg gggaatggca aagcattggc acaatcctgg
      601 tggggactct atcgtcgtcg gagcggcaga cgtttccgaa ggagcgctgg tttggttcaa
      661 gaagaacgtc aacgagaacg ggtttgtcac caaagaccac cgcgaactgc ttgaccgcaa
      721 ggacattgac gcgattgcgg tgatgagtcc cgactacaca catgagcaat atgtctgtga
      781 tgcatttgat gcgggcaaac acgttttcac cgagaagccc atggcaataa ccaccgaagg
      841 ctgcgaccgg atgctgcgcg cctgggtcgc ctccggaaag cagttcatga ttggcttcaa
      901 catgcgctac atgaacatct ttcgcgtcat gaaggacgct attgattcgg ggagtattgg
      961 tgaggtgaaa gcagtctggg tgcggcactt tgtcgcccac ggcggccgtt ggtactacca
     1021 tgactggcac ggcacgtcgc gcaacaccac cggccttctg ctccaaaagg cgtcacacga
     1081 tctggacatg atccattgga tcaccggcag atacacgacg cgtgtcgccg catccggcgg
     1141 actggactac tacggcggag acaagcccaa cgacctgcac tgcattgcat gtcctgagaa
     1201 ggacacctgc gtggagtacg accatgtcaa cgccgcctcg gacaacaaaa tggatttatg
     1261 tgtgttccgc aaagaggtgg acgtggaaga caacagcatc gtcatgatgg aactggaagg
     1321 cggtatccac gccagttaca tgcagtgcca cttcaccccg gactactgcc gcaactatac
     1381 cttcatcggc acagaaggac ggttggagaa tctcgatgac ggttcgaaag tcatcatacg
     1441 aatgcgtgac cgctcgaaac gatggcgcaa tctatctcac tacgatctgg atgtgaaacc
     1501 agcgtccggc ggacatggcg gagccgatcc tctagtgtgc cgggatttcg tcgagatgct
     1561 gttgcacggt aagcgcccac tggcaacacc actcgcagga cggatgagcg ttgccacagg
     1621 atgtgccgcc acgtggtcat tgcgaaacgg ctggcagccg gtgacaatcc caccagttcc
     1681 tgaagatatt cgggaaaagg tctacta
//
LOCUS       DAOQUC010000987         1704 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_94064_length_1704_cov_3.872650, whole genome shotgun sequence.
ACCESSION   DAOQUC010000987 DAOQUC010000000
VERSION     DAOQUC010000987.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1704
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94064_length_1704_cov_3.872650"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /gene="fliW"
                     /locus_tag="PKH07_14780"
     CDS             <1..222
                     /gene="fliW"
                     /locus_tag="PKH07_14780"
                     /inference="COORDINATES: protein motif:HMM:NF014660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HNT36247.1"
                     /translation="EGLDLTDPLEAKVLAIVVVPSDPQQATMNLKGPLVINPSSRHGK
                     QIVLADPRFSTQHPIWPQASGRELTKCSS"
     gene            210..470
                     /gene="csrA"
                     /locus_tag="PKH07_14785"
     CDS             210..470
                     /gene="csrA"
                     /locus_tag="PKH07_14785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002774103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HNT36248.1"
                     /translation="MLVLSRKPDESILIGDQIEVVVVEVKGDQVKLGVRAPRSISVHR
                     KEVYEAIQAANLEASKPQVQHLQGLAEVLRKKQEKAPEESAR"
     gene            complement(491..>1704)
                     /locus_tag="PKH07_14790"
     CDS             complement(491..>1704)
                     /locus_tag="PKH07_14790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNT36249.1"
                     /translation="FPPGAVLRSVPQNIPLLVGTNAIEDERIARCLDRIRATRPISVQ
                     VSVDGTAETHDRVRGVAGNHDAAVRLLKWCQREGIPSTVSFTLGADNYADLLAVQSLA
                     GQLDAHFAFRPVNRGEFYGNQDGHFAPSDFAEAQIQTVEQDVTALIKQKMREGYYNQT
                     DFVYWGLIPDYLRGTVKYPRCLAAKDFFLIDPAGVLYPCPQYWQPMGNVHDWPEAFMS
                     PRRLEVAAKVERLECGGCWNDCFVWNSLCRQPQWVRTQFARILHERIGVPQQAPSEIL
                     PGEDSAVACLGQGWFPSDSPGARWTSLEAALFISGGSMLHVTLSNPCPQRQSAPTQLE
                     VEVDGNPSATVPLDFQGERTLDIEMPRKSENTIREVLLKCDRTWTPASLGLSKDSRKL
                     GVAVIRVWTTQ"
BASE COUNT          385 a          477 c          471 g          371 t
ORIGIN      
        1 gaaggcttgg acttgacgga tcccttggag gccaaagtcc tggccattgt ggtggtgcct
       61 tccgacccgc agcaggcgac catgaacctg aagggaccat tggtaatcaa cccgagcagt
      121 cgtcatggaa aacagatcgt attggcggat ccgcgtttca gcacacagca tccaatttgg
      181 cctcaggcca gcggtcggga gttgacaaaa tgctcgtcct gagtcgcaag cccgacgaaa
      241 gcatcctcat cggtgaccaa attgaagtgg tcgtagttga ggtcaagggt gaccaggtga
      301 aactgggtgt ccgtgcgcct cgttccattt ccgttcatcg taaagaagtc tacgaagcca
      361 ttcaggcagc caatcttgaa gcctcaaaac cccaggttca gcatcttcaa ggactggctg
      421 aggtcttgcg caagaaacaa gaaaaggcac ccgaagaatc cgctcggtag gcagagtttc
      481 cctgacgcga tcactgcgtt gtccatactc gtatgacagc gactccgagt ttcctgctgt
      541 ctttcgatag tcccagactg gcgggagtcc aggttcgatc acacttgagc aagacttccc
      601 ttatcgtgtt ttctgatttc ctcggcatct ctatgtcgag cgttctctcc ccttgaaagt
      661 ccaagggcac cgtggcagat gggttcccgt ccacctcgac ttccagttgc gttggcgcag
      721 actggcgttg tgggcaggga ttggaaagcg tcacgtgcag catggagccc cctgagatga
      781 acaacgccgc ttccaaactt gtccagcgcg ctcccgggct gtctgaggga aaccagccct
      841 gccccaggca ggcaacagca gaatcctcgc cgggaagaat ctcggaagga gcctgttggg
      901 gaactccaat tctttcgtgg agtattctgg caaactgcgt tctgacccac tgtggctgcc
      961 gacagagact gttccagacg aagcagtcgt tccagcaacc gccgcattca agtcgttcca
     1021 ctttcgcagc aacttccaaa cgccgaggtg acataaaagc ctcaggccag tcgtgaacat
     1081 tgcccatcgg ctgccaatac tgcggacagg gatacagaac acctgcaggg tcaatgagga
     1141 aaaaatcctt tgcggcaaga catcgtgggt atttgacggt gccccgaagg taatcgggga
     1201 tcaagcccca gtagacaaaa tccgtctggt tgtaatagcc ctcgcgcatc ttttgtttga
     1261 tcagggcagt gacgtcttgc tccacagtct ggatctgcgc ttcggcaaag tcagatggtg
     1321 cgaaatgacc gtcttggttg ccgtagaatt caccgcggtt gaccggcctg aacgcaaaat
     1381 gcgcgtccaa ctgacctgca agactttgaa cggcaagcag gtcggcatag ttgtccgccc
     1441 ccagggtaaa ggataccgtt gagggaatgc cttctctttg gcaccatttg agaaggcgta
     1501 ctgcggcgtc atggtttcct gcaactccac ggactcggtc gtgagtctcg gcggtgccat
     1561 ccaccgaaac ctgaaccgaa atcggtcttg tcgcgcggat ccgatccagg catcgggcga
     1621 ttcgttcatc ctcgattgca ttggttccca ccaggagcgg gatattctga ggcaccgagc
     1681 gcaacaccgc tcctggtggg aacc
//
LOCUS       DAOQUC010000988         1704 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_94113_length_1704_cov_2.263796, whole genome shotgun sequence.
ACCESSION   DAOQUC010000988 DAOQUC010000000
VERSION     DAOQUC010000988.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1704
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94113_length_1704_cov_2.263796"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="PKH07_14795"
     CDS             <1..412
                     /locus_tag="PKH07_14795"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNT36250.1"
                     /translation="DGASAHWEFPLPNALIRVLGNESLEHGEQIRFRIVRHADKVVAG
                     GVFLHHNHYVSYFLSAFDRHWGPFCPMNLLLKGEIQYACLRGRQVFDFGASLGIESVL
                     KFKSSFGAEPVPRTRVVIRNRLWRALMSWRKTGV"
     gene            498..932
                     /gene="yidD"
                     /locus_tag="PKH07_14800"
     CDS             498..932
                     /gene="yidD"
                     /locus_tag="PKH07_14800"
                     /inference="COORDINATES: protein motif:HMM:NF013932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HNT36251.1"
                     /translation="MRYSLWVVLVLGVSSTDATRALAQPRLDLFQPEPKRAETLMSNV
                     GTFWIRVFQKFISPQDGKKCGLRPSCSEYSRQCFRQHGSFRGWLMTSDRLQRCHPKKS
                     QGFYRIREGTIIDTPHENLAREQQRCDLFAATRSSKGGSFGR"
     gene            929..>1370
                     /locus_tag="PKH07_14805"
     CDS             929..>1370
                     /locus_tag="PKH07_14805"
                     /inference="COORDINATES: protein motif:HMM:NF024572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNT36252.1"
                     /translation="MMLRALKRMVFVSVLLCGACFLPIVHAQDQTSSFEFALYLLRSG
                     EYEAARVELHRYLYFSPSGQRTQEARFRIGQCHFFLRQWDKALEAFDDCAVQSTDIEW
                     VDDALFWSARTSFRRLEYEIATDKAVAFLDAFPDSPYVDDSRAMI"
     assembly_gap    1371..1380
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1381..>1704
                     /locus_tag="PKH07_14810"
     CDS             <1381..>1704
                     /locus_tag="PKH07_14810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36253.1"
                     /translation="SAYELGRLENRCFVGDSKWEEAATAFGELAECYQDGSLEYRRSL
                     MLVKQSREARSLPRRSPSLAAVLSTLLPGAGQFYARQRGDSFFAALLNGLSIGLAVEA
                     FSKGRV"
BASE COUNT          355 a          423 c          499 g          417 t
ORIGIN      
        1 cgacggagcc tcggcacact gggagttccc actgccgaat gcgctgatac gagtcttggg
       61 taacgagtcg ctggagcacg gggaacagat acgattccga atagtgcgtc atgcggacaa
      121 ggttgttgcc ggcggcgttt ttctccacca caaccattat gtttcctatt tcctgtcggc
      181 gttcgatcgc cactggggac cgttctgtcc gatgaatctc ctgctgaagg gggaaatcca
      241 gtacgcttgc ctgagaggac gccaagtttt tgattttggc gccagccttg ggatcgagag
      301 cgtcctgaag ttcaaatcca gttttggtgc cgaaccagtt ccccgaacgc gcgtggtgat
      361 ccgcaatcgc ctttggagag ccctaatgag ttggcgcaag acgggcgttt gacaagcagc
      421 caactggatc ggtagcatcc tcgctgggga ttgggcgaga agttgctttc gacgtttgtg
      481 tttgagaggt ttgactgatg aggtactccc tctgggtcgt tctggtgctg ggtgtaagtt
      541 ccacagacgc gacgagagcc cttgcgcaac cgaggcttga cctctttcag cccgagccga
      601 aaagagcaga aacgttgatg agcaatgtgg gaacgttttg gattcgggtc tttcagaagt
      661 tcatctctcc tcaggacggc aagaagtgcg gactccggcc ttcgtgctcg gaatactcaa
      721 ggcaatgttt ccggcagcat gggtctttcc gtggttggct gatgacgtca gacagactgc
      781 aacgctgtca tcccaaaaaa agtcagggat tctaccgaat cagagaaggg acaatcatag
      841 acactcccca cgagaatctc gcacgcgaac agcagcgctg tgatcttttc gctgctacaa
      901 ggtcatccaa aggggggagt tttggacgat gatgttacga gcattgaaac gaatggtgtt
      961 tgtgagtgtg ctcctctgtg gcgcgtgctt tttgccaatc gttcatgccc aggatcagac
     1021 aagttcgttt gagtttgcgc tgtatcttct ccgctcggga gaatatgagg cagcccgagt
     1081 tgagttgcac cgttatctgt acttttctcc ttccggacag cggacacagg aagcacgatt
     1141 tcgtattggc cagtgccact tttttctcag acagtgggac aaggcgctgg aagcctttga
     1201 tgactgcgca gttcagtcca cagacatcga gtgggtggac gatgcacttt tctggtcggc
     1261 aagaacttcg ttccggcgtt tggagtacga gatcgcaacg gacaaggcgg tggcttttct
     1321 ggatgcgttt cccgacagcc cctatgtaga tgactctcgt gccatgatcg nnnnnnnnnn
     1381 agcgcgtatg agctgggtag actcgagaat cgatgttttg tcggcgacag caaatgggag
     1441 gaagcggcaa cggcattcgg ggaacttgcc gagtgctatc aggatgggag tctcgaatat
     1501 cgccggagtc tcatgttggt caagcaatca agagaggcga gaagtctccc gagacgctcc
     1561 ccttctctcg cagcggttct ttccacgttg cttccaggcg ctggtcagtt ctatgcccgc
     1621 cagcgaggag actctttctt cgcagcgctt ctgaacggcc tgtccatcgg cttggcagtc
     1681 gaagcctttt caaaagggcg agtc
//
LOCUS       DAOQUC010000989         1700 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_94445_length_1700_cov_4.353191, whole genome shotgun sequence.
ACCESSION   DAOQUC010000989 DAOQUC010000000
VERSION     DAOQUC010000989.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1700
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94445_length_1700_cov_4.353191"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /locus_tag="PKH07_14815"
     CDS             complement(<1..235)
                     /locus_tag="PKH07_14815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36254.1"
                     /translation="MEPIVNVITTLTRIQGEGGAEQYVGTIDMSALRKAKIGRLEIVV
                     VPLCTVPKTLSDLINKACVANADALMFGALARQE"
     gene            complement(241..546)
                     /locus_tag="PKH07_14820"
     CDS             complement(241..546)
                     /locus_tag="PKH07_14820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36255.1"
                     /translation="MQEYQRIACFRLAVHLKKLGMPPDIAIVCLQAWAAKNKPHEPKG
                     IITHEEILSQVQAAFEKPYLGCGCNEPTVRQFCDPNCPLQMRRTIESTDSSCQRKEQ"
     gene            complement(752..>1700)
                     /locus_tag="PKH07_14825"
     CDS             complement(752..>1700)
                     /locus_tag="PKH07_14825"
                     /inference="COORDINATES: protein motif:HMM:NF025063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HNT36256.1"
                     /translation="ALRHQLAIYRGQVKRARIGPTDRLFWVWLSKVWLGWRDALVFVK
                     PSTVIAWQRRRFQDHWTSLSRNGKPGRPGLVKEIKDLIRKISTANPTWGSPRIVGELG
                     KLGIELSKSTVEKYMVRVRKPPSPTWKSFLKNHVKDLVSIDFFVVPTLRFKILFVLVV
                     LAHDRRRVVHFNVTEHPIAQWTAQQMVEAFPWNARPRYLLRDRDAVYGQEFRDRVKHF
                     GIDEVITAPRSPWQNPYAERLIGSIRRECLDHVIVLNERHLKRILSDYFGYYHRWRTH
                     LSLTMDCPEPRFIQFPESGKVVEVREVNGLHHHYERRAA"
BASE COUNT          381 a          460 c          441 g          418 t
ORIGIN      
        1 cttcctgacg agcgagcgcg ccaaacatga gggcgtcagc gttcgcaacg caggccttgt
       61 tgatgagatc cgaaagagtt tttggaacgg tgcacaatgg gactacgacg atctccaacc
      121 tcccgatctt cgccttcctc aaggcggaca tgtcgatagt cccgacgtat tgctccgctc
      181 ctccttctcc ctggattctc gtgagggtcg tgatgacgtt gacgattggt tccatgatac
      241 ctattgctcc tttcgctgac atgacgagtc cgtggactcg atggttcttc tcatctgcag
      301 agggcagttt gggtcgcaga attgccgaac tgttggttca ttgcacccgc agcccagata
      361 gggtttttcg aatgcggcct gcacctgcga gaggatctcc tcatgcgtga tgattccctt
      421 tggctcgtgc ggtttgttct tagccgccca ggcctgaaga cagacaatgg cgatgtcggg
      481 aggcataccc agtttcttta ggtgaactgc caacctgaag caggcgatcc gctggtactc
      541 ctgcacttgt ggttcccgaa aactcatatg acgtctaaaa cagaggaact ggccctcagt
      601 cgtacacaag attgggagat tcacgaggtc gttcgcaagc ggttgggatg ttcctgcgat
      661 cctggtgaga tatctcccaa cggcggatag agttctccct cactttgcgg taaaatctgc
      721 cgtattcatg aggtatgtgg cgaagtcgaa cttatgcagc gcgacgctcg taatgatgat
      781 ggagaccgtt cacttcccga acttccacaa cctttccaga ctcgggaaac tgaatgaagc
      841 gtggctccgg gcaatccatg gtcagagaga gatgtgttcg ccaacgatga tagtagccaa
      901 agtaatcgga tagaatccgt ttgagatgcc tttcattaag aacgatgacg tggtccaggc
      961 attctcgccg aatgctaccg atcagccttt ccgcgtaggg attctgccaa gggctcctgg
     1021 gggcggtgat gacctcatcg atcccgaaat gcttcacccg atctcggaat tcttgaccat
     1081 atacagcatc tctgtctcga agtagatacc gaggtcttgc attccacgga aaggcttcca
     1141 ccatctgctg tgctgtccat tgggcaattg ggtgttcagt cacattgaaa tgtaccacac
     1201 ggcgccgatc atgtgccagc acaacaagta cgaaaagaat cttgaatctc agtgtcggaa
     1261 ccacaaagaa gtcgatcgat accaagtctt tcacgtggtt ctttagaaag gatttccagg
     1321 tcggggatgg cggcttacgc acgcgcacca tgtacttctc gacagttgac ttggacagtt
     1381 cgatccccag tttcccaagt tcgcctacaa ttcggggaga accccaggtc ggattcgccg
     1441 tggaaatctt acggatcagg tctttgatct cttttaccag acctgggcgc ccgggcttgc
     1501 cattcctgct caaacttgtc cagtgatcct gaaaccttct tcgttgccaa gcgatgacgg
     1561 tgcttggctt gacgaagacc aacgcatctc gccaccccaa ccagactttg gacaaccata
     1621 cccagaacag ccgatcagta ggtccaattc gggcacgctt cacttggcct cgatatatgg
     1681 ccagctggtg gcgcaaggcc
//
LOCUS       DAOQUC010000990         1699 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_94548_length_1699_cov_3.059611, whole genome shotgun sequence.
ACCESSION   DAOQUC010000990 DAOQUC010000000
VERSION     DAOQUC010000990.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1699)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1699)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1699
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94548_length_1699_cov_3.059611"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..585)
                     /locus_tag="PKH07_14830"
     CDS             complement(<1..585)
                     /locus_tag="PKH07_14830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rnf-Nqr domain containing protein"
                     /protein_id="HNT36257.1"
                     /translation="MADARQKKLGAVFLDSFWRDNAIFRQILGICSTLAVTNLVVNTV
                     FMCLGLTFATAMSSATVSALRAYTPKRVRMMVQTLVIAAYVIIIDIVLKAYVPTVSKA
                     LGPYVGLIITNCIIMGRCEAFAGSNPPRPSFVDGLGSGLGYSVVLLAIAVVRELLGFG
                     TILGFTVLGGTWTRWTIMVMPAGAFFMLGLMIWLA"
     gene            complement(588..1238)
                     /locus_tag="PKH07_14835"
     CDS             complement(588..1238)
                     /locus_tag="PKH07_14835"
                     /inference="COORDINATES: protein motif:HMM:NF016121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HNT36258.1"
                     /translation="MNKALGTVLYMVILTAVFTGAVTVVGEATKDRIAVNESLTERRK
                     ILLALGVTEDERLSAGEVERVFRNNIREMPWKGHERAVYGRLNERGVAEAYAFPIEGP
                     GFWGPIYGYLGVSADGAEIIGLVFIRHSETPGLGARIEELWFRKQFQGKSIKPSGGTS
                     ALSFVAQGRELDERSVHAITGATQTSRALERFLDEDLKEIHRAFEQGGLSLGEGVR"
     gene            complement(1235..>1699)
                     /locus_tag="PKH07_14840"
     CDS             complement(1235..>1699)
                     /locus_tag="PKH07_14840"
                     /inference="COORDINATES: protein motif:HMM:NF015100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HNT36259.1"
                     /translation="SYQYNLWDLYLGNVGGSIGETCALAIVLGGFFLLLRKVASWRVM
                     LGVALGAGLVSVLGSLFGVEKIPDPLFTFGSGGLLFGLVFMATDPVSGSSTNPGKWIM
                     GFGVGALTVLLRSFSIFAGAVMFSILLMNTFTPLIDFLVKEYVQSKKVRSTK"
BASE COUNT          466 a          516 c          391 g          326 t
ORIGIN      
        1 cgccagccat atcatgagac ccagcatgaa gaaagccccc gcaggcatca ccatgatcgt
       61 ccagcgtgtc caggttccac ccaggactgt aaatccaaga attgttccga acccgagaag
      121 ttcacgaacc acggcgatgg caagcagcac cacagagtaa cccagtccac tgccaagacc
      181 atccacaaaa ctcggtcttg ggggattgct gcctgcaaag gcctcacacc ttcccatgat
      241 gatgcagttg gtgataatca aacccacata aggacccaat gccttggaga cggtcggaac
      301 ataggctttg agcacaatgt caatgatgat gacataggcg gcgatgacga gcgtttgaac
      361 catcatccga acccgcttgg gcgtataggc acgcaaggcc gagacagtcg cagaagacat
      421 cgccgtcgcg aaagtcaatc caagacacat gaaaaccgtg ttgacaacaa ggttggtcac
      481 agcgagcgtc gaacaaatgc ccagtatctg acgaaatatc gcattgtcgc gccagaatga
      541 atccaagaat accgcaccca gtttcttttg cctggcatct gccatagtca acgcactcct
      601 tctccaagcg aaagaccgcc ctgctcaaat gcccggtgaa tctctttcaa atcctcatcg
      661 agaaagcgct ccaaagctct gctggtctga gtcgcgcccg tgattgcatg aacggaacgc
      721 tcgtcgagct cccgcccttg ggcaacaaaa ctcaatgcag atgtaccgcc tgagggcttg
      781 atagatttgc cctgaaactg tttgcgaaac cagagttcct cgatgcgcgc cccaagtccg
      841 ggcgtttcac tgtgccgaat gaaaacaaga ccaatgatct cggctccatc ggctgatact
      901 cccagatagc cgtatatcgg cccccagaaa cctggccctt caatgggaaa agcgtaggcc
      961 tctgcgacgc cccgctcatt cagccggcca tagaccgctc gctcgtgccc cttccaaggc
     1021 atttccctta tgttattccg aaaaactcgt tccacctctc cagcgctcag cctctcatcc
     1081 tcagtcaccc caagcgcaag cagaatcttg cgccgctcag tgagcgattc attcacggca
     1141 atccgatcct tggtggcttc tccgacgact gtcacagcac cggtgaagac cgccgtgagg
     1201 atgaccatgt acagaactgt gccaagtgct ttgttcactt cgtgctccga acctttttcg
     1261 actggacata ttccttgaca agaaaatcaa tcagaggcgt gaaggtgttc atgagcagaa
     1321 tcgagaacat caccgcgcca gcgaaaatag agaaacttcg aagaagcacc gtcaacgctc
     1381 caacaccaaa gcccataatc catttgcctg ggttggtgct ggaaccgctg accggatcgg
     1441 tggccatgaa taccagccca aagagaagcc caccactccc aaaagtgaac aggggatcgg
     1501 gaatcttctc aacaccaaac agcgaaccga gaacggaaac gagccccgct ccaagagcca
     1561 ctccaagcat cactctccag gaagcgacct ttctcagaag gagaaaaaag ccgccaagta
     1621 caatcgccaa agcacacgtt tcgccgattg aaccccctac attcccaagg tagagatccc
     1681 acaggttgta ttgatatga
//
LOCUS       DAOQUC010000991         1699 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_94569_length_1699_cov_2.591849, whole genome shotgun sequence.
ACCESSION   DAOQUC010000991 DAOQUC010000000
VERSION     DAOQUC010000991.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1699)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1699)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1699
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94569_length_1699_cov_2.591849"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            30..323
                     /locus_tag="PKH07_14845"
     CDS             30..323
                     /locus_tag="PKH07_14845"
                     /inference="COORDINATES: protein motif:HMM:NF012442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HNT36260.1"
                     /translation="MTKKEIIDMVAERTGQKHSHTKAVVEQVLEVFIDKLAEEGRIEI
                     RNFGVFKVKRTPARIGRNPKTREVAPVPARNIIQFKAGKTMKERVNVQQDDAE"
     gene            339..737
                     /locus_tag="PKH07_14850"
     CDS             339..737
                     /locus_tag="PKH07_14850"
                     /inference="COORDINATES: protein motif:HMM:NF015721.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HNT36261.1"
                     /translation="MEKPIQPNKDENPRIGDEVLRSIALLCASEIEGVSCTSDNLMKS
                     VTNLLRPGAPHKGVRVTPNEEDNSLTIDLSISVSQGKSIPSLVKGLQSAVKDAVEKMT
                     NFRVTAVNVNVSDIDWETEQPAATEQGKEE"
     gene            748..1077
                     /locus_tag="PKH07_14855"
     CDS             748..1077
                     /locus_tag="PKH07_14855"
                     /inference="COORDINATES: protein motif:HMM:NF037081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HNT36262.1"
                     /translation="MTNQDNALPMTITKKEIIKEVSDRTGMDLHTVRDMVQAMFDCMC
                     EGLSNDQHIEIRNFGIFKVKKTPKRKARNPKNKTVITVPGKRHIHFKPGRLMKIRINQ
                     TTGESSS"
     gene            1102..>1699
                     /locus_tag="PKH07_14860"
     CDS             1102..>1699
                     /locus_tag="PKH07_14860"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HNT36263.1"
                     /translation="MSSDELIKRILKEVGKMPPLPDVLTKVMQMTKDPDVSASQMNKV
                     ISMDQALTANILKLCNSAYYGLPRVISSVTQAVMYLGFQTVRNLVMTCTMQDVYAGQM
                     QGYGYPEGGLWKHSVSVAIASQILCKKIRPGLNDTAFTAGLLHDLGKTILNKHVGGRF
                     QEVAAVMQQKNAMMHEAEAEVFGFDHAIIGAKIADQWNF"
BASE COUNT          492 a          424 c          446 g          337 t
ORIGIN      
        1 gggtaggctt tcggcttaag gaggagtgaa tgacaaaaaa agaaattatt gatatggttg
       61 cagaacggac tggccagaag cacagccaca ccaaagcggt tgttgagcag gtgctggaag
      121 tctttattga caagcttgcg gaagagggac gtattgagat taggaacttt ggtgttttta
      181 aggttaagag aacgccggcg cgcatcgggc gaaacccgaa gacccgcgag gttgcacccg
      241 tacccgctcg aaacatcatc cagttcaaag cgggcaagac aatgaaggaa agagtgaacg
      301 tccagcagga cgatgccgag taaatgcagg aggacatcat ggaaaagccc attcaaccca
      361 acaaagatga gaatccgagg atcggtgacg aggttctgcg ctctatcgct ctgctctgtg
      421 cgtctgaaat agagggagtc tcttgcacga gcgacaatct gatgaagagc gtgacaaatc
      481 ttctgcgtcc gggtgctcca cacaagggag tccgggtcac accaaacgag gaagacaaca
      541 gcctcacgat cgatctttcc atttcggtca gtcaagggaa atccattccc tccttggtca
      601 agggacttca gagtgctgtc aaagatgccg tcgagaagat gactaacttc cgtgtgaccg
      661 ccgtcaacgt caatgtctcg gacatagact gggagaccga gcagccggca gccacagagc
      721 aaggcaagga ggagtagttc cgcaaccatg acaaaccaag acaacgccct tccgatgacc
      781 ataacgaaga aggagattat caaagaagtc tccgatagaa ccggtatgga tctgcacaca
      841 gtaagagata tggttcaggc aatgttcgac tgcatgtgtg agggactctc caacgatcag
      901 catatcgaga tcagaaactt cgggattttt aaggtcaaga agactcccaa acgcaaggcc
      961 cgtaacccaa agaacaagac agtgatcaca gtgcctggca agaggcacat ccacttcaaa
     1021 cccggtcgtc tgatgaagat tcgcatcaac cagacaactg gggagtcctc gtcctagagc
     1081 cgattaggag agaccgacac aatgtccagt gacgaactca ttaagagaat actcaaagag
     1141 gtcggcaaga tgcctccctt gcccgacgtc ctcacgaagg tcatgcagat gaccaaggat
     1201 ccggacgttt ctgcgtccca gatgaacaag gtcatatcca tggaccaggc tttgactgca
     1261 aacatcctga aactctgcaa ttccgcctat tatggactgc ccagggttat ctcgtcagtc
     1321 acacaggccg tcatgtacct cggatttcaa acggtcagga accttgtcat gacctgcaca
     1381 atgcaggacg tctatgccgg acaaatgcaa ggctatggat atccagaggg tggcctttgg
     1441 aagcattcgg tctcagtagc catcgcatcg cagattctct gcaagaaaat ccgtccaggt
     1501 ctcaatgaca cggcctttac tgccggactt cttcacgacc tgggaaagac aattctcaac
     1561 aaacacgttg gcggacgctt tcaggaagtt gccgccgtca tgcagcaaaa gaacgcgatg
     1621 atgcatgaag ccgaggcgga agtctttgga tttgaccatg ccataatcgg tgcaaagatc
     1681 gccgaccagt ggaacttcc
//
LOCUS       DAOQUC010000992         1698 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_94686_length_1698_cov_2.570907, whole genome shotgun sequence.
ACCESSION   DAOQUC010000992 DAOQUC010000000
VERSION     DAOQUC010000992.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1698
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94686_length_1698_cov_2.570907"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="PKH07_14865"
     CDS             complement(<1..234)
                     /locus_tag="PKH07_14865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36264.1"
                     /translation="MVCENCRYQFHFLYDHKKSRKATATTEAGRIRWDLASLKSRYVD
                     LHACYQPCPHCGYVQEWMVKAKRWARIQDYGLIG"
     gene            complement(685..1518)
                     /locus_tag="PKH07_14870"
     CDS             complement(685..1518)
                     /locus_tag="PKH07_14870"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HNT36265.1"
                     /translation="MDIIPSEDCMASAYLLAFVFGSCVGSFLNVVIYRIPIGKSVLHP
                     RRSFCPQCEHGIAWYDNVPILSYLLLGRKCRHCHKPISFRYCLVEALTGLLFCLVLHR
                     FGITPATPVYWVLTAGLIAITFVDLDHFIIPDSISLGLLPVGLILSFAAFFYGPFEGL
                     HVTNPIQALLGLLVGWSVLDIIGEYATVFLKRDAMGRGDVKLMGMVGTFLGWELTLAA
                     IPVSAFVGLICYLPKLVTRKIGRFQEIPYGPYLAVSSFVLMLYGDRWIDWYLRLIGIL
                     E"
BASE COUNT          469 a          446 c          405 g          378 t
ORIGIN      
        1 tccaatgagg ccatagtcct ggatacgtgc ccatcgtttg gccttgacca tccactcctg
       61 cacgtagccg cagtggggac acggctgata acatgcatgc aagtccacat agcgactctt
      121 cagcgaagcc agatcccatc gaatccgacc tgcttcagtc gtcgcagtgg ccttgcgact
      181 cttcttgtgg tcgtacagga agtggaactg ataccggcag ttctcacaga ccacacgttc
      241 tctttcaaca agcccgttct gccctgtttt ccttgttttt tcttctcgct gctttgccat
      301 ttctgcaacc gccctttctg ggatgccacg ctgcatacaa aacaaaaaac acgcttcagc
      361 ccttacgcaa ctcggatgcc aacaattcca cttaggcata ataactttag aaaatactag
      421 agaatatcaa atataaaagc atttatatca atatgatata gattttgaaa atatccgaaa
      481 atactctgat ccttcccact gaaagtctgc cagatgctga ataatgcctg ggaatttcgt
      541 tcctctccta tgcacagaga gggcaggaaa ttccagtgtt tgatggttga tagagatgct
      601 ctgtggatgt gaaaacggca gtaggagatg tcaagcgacg cgatatattg atatgcttcg
      661 gttcgaggat cgaaggtgtg aaacctactc cagtattcca atcagtctga gataccagtc
      721 aatccatctg tcgccgtaga gcatcaaaac aaaagagctg acggcgagat aggggccgta
      781 agggatctct tggaaccggc cgatcttgcg agtcaccagt ttgggcagat aacagataag
      841 ccccacgaat gccgataccg gaatggccgc cagtgtcagc tcccaaccca ggaaggttcc
      901 gaccattccc atcagtttca cgtcacctcg tcccatggca tcgcgcttca ggaagactgt
      961 cgcatattcc ccaatgatat ccaaaactga ccaacccacc aaaagaccga ggagagcctg
     1021 aattgggttg gtgacatgca gcccttcgaa aggaccataa aaaaaggccg caaagctgag
     1081 tatcaatcca acgggcagca gtccaagact gatcgaatca gggatgataa agtggtcgag
     1141 atccacaaac gtgattgcga tcagcccagc cgtcagcacc cagtagaccg gcgttgccgg
     1201 tgtaatcccg aagcggtgca gcaccaggca gaacagcaat ccggtcagcg cctcgaccag
     1261 acagtatcga aaactgattg gcttgtggca gtgacggcat tttctcccca aaagaagata
     1321 cgaaaggatc ggtacgttgt cataccaggc gataccatgc tcgcattgag gacaaaagga
     1381 acgccttggg tggagaaccg acttcccgat gggaatccgg tagatcacaa cgtttagaaa
     1441 actaccgacg caggagccga agacaaaagc cagaagatat gccgacgcca tacagtcctc
     1501 cgaggggatt atgtccatca gggcatcgtc tttgtccgat gaaccttgtg aacccctttc
     1561 attctgatga ttcttcctga ttcgtgaagt actctatcct gcactgacaa aagagatgca
     1621 gagatgaatg gtgggcccgg cagggctcga acctgcgacc aagggattat gagtcccctg
     1681 ctctgaccaa ctgagcta
//
LOCUS       DAOQUC010000993         1695 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_94914_length_1695_cov_3.903049, whole genome shotgun sequence.
ACCESSION   DAOQUC010000993 DAOQUC010000000
VERSION     DAOQUC010000993.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1695
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94914_length_1695_cov_3.903049"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1440
                     /locus_tag="PKH07_14875"
     CDS             <1..1440
                     /locus_tag="PKH07_14875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013152135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HNT36266.1"
                     /translation="IMVAFEDQRAAVAQPGDEVTIVVRARDFPQPPQPRGKQSQRYER
                     HGCHYTRTTGIWQTVWMEPVSRTSLLRTRLTPDVANSCVHLEQPIRNAKSGTRVRAVL
                     SDQQGPISAAEAPADLDFAPRLTLGIPEQRRRLWSISDPHLYQIGVELLDANGKVMDQ
                     ISSYAGLRSVSIEGKAIKINGTSVFQRLVLDQGYYPDGILTAPTEEVLIKDIELSQAV
                     GFNGARLHQKVFEERFLYHCDRLGYIVWGEFPDWGGQGYGRDDDHWQPGATYITQWLE
                     EIERDYSHPSIIGWCPLNETWQTLHDGITVLDDVTRGMFLATKAMDTTRPVLDASGYS
                     HRVREADVYDSHDYNQDPAKFKETHAGLAQDKPFLNTHGHRVISIPYAGQPYFVSEFG
                     GIWWNPNAKPGEDSWGYGERPKTLEEFYTRFEGLCRALLEDAGMFGYCYTQLTDVYQE
                     QNGIFTFDRRNKFDLERMRRIQQQKAAIE"
BASE COUNT          392 a          492 c          445 g          366 t
ORIGIN      
        1 atcatggttg ctttcgaaga tcagcgcgca gcagttgcgc agccgggaga tgaggtgacg
       61 atcgtcgttc gcgctcgaga tttcccgcag cctccacaac cccgtggtaa gcagtcgcaa
      121 cgctacgaac gccacggttg tcactacaca cgcacgacgg gaatctggca gaccgtttgg
      181 atggagccgg tctcgcgcac ttcactgctt cgaactcgct tgactcctga tgttgccaac
      241 agctgcgttc acctggagca gcccattcga aacgcgaaat ccggaaccag ggttcgcgca
      301 gtcctttctg atcaacaggg gccgatcagt gcggcagaag cacccgcgga cttggacttt
      361 gccccacgcc tgactttggg cattcccgaa cagcgtcgaa ggctctggtc tatcagcgac
      421 ccgcacctct atcaaatcgg ggttgaactt ttggatgcca acggcaaagt gatggatcaa
      481 atctccagtt atgctggtct ccgaagcgtg tccatcgaag gcaaggcgat caagatcaat
      541 ggcacgtccg tgtttcagcg gctggtgctc gaccagggat actatcccga cggcattctc
      601 accgcaccaa ccgaagaagt cctcatcaag gatatcgaac tgagtcaggc tgtcggcttc
      661 aatggagcaa gactccatca gaaagtcttt gaggagcgat ttctctatca ctgcgaccgg
      721 ctcggataca ttgtctgggg cgaattcccc gactggggtg gacagggcta tggccgcgac
      781 gatgaccact ggcagcctgg agcgacttat atcacccaat ggctcgaaga aatcgaacga
      841 gattactccc atccgagcat catcggctgg tgtccgctga acgaaacttg gcagaccttg
      901 catgatggaa tcaccgttct ggatgatgtc acacgcggca tgtttctcgc caccaaagca
      961 atggacacga cacgccccgt tttagatgct tccggctatt cgcacagagt tcgagaagcc
     1021 gacgtctacg actcacacga ctacaaccaa gatcccgcga agttcaagga gacgcatgca
     1081 gggcttgccc aagacaaacc cttcctcaac acacacgggc atcgggtgat ctccattccc
     1141 tatgctggtc agccctactt tgtcagcgag ttcggcggca tctggtggaa ccccaacgcc
     1201 aagccaggag aagactcctg gggttacggc gagcgaccca agacccttga ggaattctac
     1261 acccgctttg aaggactctg tcgagcactc ttggaagatg ccggtatgtt cggctactgc
     1321 tacacccagt tgaccgatgt gtaccaggag cagaacggca ttttcacctt cgatcgcaga
     1381 aacaagttcg atctggaaag aatgcgcagg atccagcaac agaaagcggc gatcgaatag
     1441 cgatctttcg gctggaagaa caggacagat tttacacaga ggacgtatcg gctcttagcg
     1501 attcggtgga tcgtttcctc tttcgattca tgttctgagt cgcgcggagt gtacttctgc
     1561 gcaatcatct ctctctgaga gagtcatttc tgactgcctc ctcccaacag cgccttgttc
     1621 atctcagcag tcttcactgc gcccagcgca tgcgtcagga tccgtttgat cgcttcctga
     1681 tcgctcagag cccag
//
LOCUS       DAOQUC010000994         1694 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_95012_length_1694_cov_3.499085, whole genome shotgun sequence.
ACCESSION   DAOQUC010000994 DAOQUC010000000
VERSION     DAOQUC010000994.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1694
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95012_length_1694_cov_3.499085"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1694
                     /locus_tag="PKH07_14880"
     CDS             <1..>1694
                     /locus_tag="PKH07_14880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36267.1"
                     /translation="PAEPQINLRGTRVHPAVNMSRYYQASIVYQFPENHATIADKKFQ
                     TNAAGKATVLYSELIPEGTIIRFEVVQTVDRSTRVTTNPQNIGDRIVDPQHDYDCYPC
                     GFVYSGTVYDPSLYNKDVFLGSVAYDGSDMAVIPVNRGSIEMWWYETSQDVCWPVRPV
                     IYQCDWPAVPDNCIIIANGKGIPGFPQAKYLNPEIYWKGTLTSDANEIGYNPNEEHAE
                     WIQIPGENLHAARVDYNVVSARSEPYVLIRYQDNEKPNKPWEFDVIRVVAESPMGAPT
                     SGCACQAEPCTFVYSTTAGYSLAPPIPLLFHIPYCAQNTIINTPVQNYFWDDQKGRLW
                     FRRGNTSVTARFFENWQGNCTPWLDYGTGVPQNIVYNLKWPSRAADEQNPPGSPNTYT
                     PLAFGETRDRTGYGIAEVLYNDCDASLIMPWRLSYVDLPSTDVPDDYISCHSSLPPHI
                     APRLDYDDVNQVLFFRGVRMDGLPGIMSESDRAAIRLAFAGKTKFLEAVDKLYTESQQ
                     TVAKAVGTKVGMENPGWGIAVSTGSATKPGWLVVGYNGHPDVAEPAEVEIFYVGCP"
BASE COUNT          397 a          450 c          458 g          389 t
ORIGIN      
        1 ggcctgcgga gcctcagatc aatctcagag gcaccagggt gcaccccgcg gtcaatatgt
       61 ccaggtacta tcaggcgagc attgtctacc agttccctga gaaccacgcg accattgctg
      121 acaaaaaatt ccagacaaat gcggctggca aggctactgt tctctattca gaactgattc
      181 cggaaggcac gattatcagg tttgaggtcg tgcagactgt ggatcgcagc acccgggtga
      241 ctacaaatcc ccagaacatc ggagatcgaa tcgtagaccc acagcacgac tacgactgct
      301 atccgtgtgg atttgtctac tcaggaacag tgtatgatcc atcgctgtac aacaaagatg
      361 tcttccttgg atctgttgcc tacgatggtt ctgacatggc agtgattccg gtcaaccgcg
      421 gcagtattga aatgtggtgg tatgagacat cccaagacgt gtgttggccg gtaagaccgg
      481 tgatatacca gtgtgactgg cctgcggtgc cggataactg catcatcatc gccaatggga
      541 agggtatccc tggatttcct caggcgaagt atctgaatcc cgagatatac tggaagggta
      601 ctctcacaag cgatgcgaac gagatcggtt acaatcctaa cgaagagcat gctgagtgga
      661 ttcagattcc gggggagaat ctgcacgctg ctcgcgttga ctacaacgtg gtctctgcac
      721 gttcagaacc ttatgtcttg attcgctacc aggacaatga gaagcccaac aaaccttggg
      781 agttcgatgt catccgagtt gtggcagagt ctccaatggg ggctcccact tccggatgcg
      841 cctgtcaggc agaaccatgc acgtttgtct actccacaac cgcgggctac agcttggcgc
      901 cgccgatccc tctgctgttc cacatcccgt actgcgctca gaacacaatt atcaacacac
      961 cggtgcagaa ctacttctgg gatgaccaga agggacgcct gtggttccgc cgaggcaaca
     1021 cgagtgtcac cgctcgcttc tttgagaact ggcaagggaa ctgcacgcct tggttggatt
     1081 acgggacggg ggtcccgcag aacattgtct ataacctcaa gtggccctct cgtgcagcgg
     1141 atgaacagaa cccgcccggc agtccgaaca cttatacgcc tcttgcgttt ggcgaaacaa
     1201 gagatcgaac tggctatggt atcgctgaag tgctttacaa cgactgcgat gcttcgctca
     1261 tcatgccttg gcggttgtcc tacgttgacc tgccatctac tgatgtgccg gatgactaca
     1321 tatcttgtca tagctcactt ccgccacaca ttgctcctcg gctcgactac gacgacgtca
     1381 atcaggtgct cttcttcaga ggagtcagga tggacggtct gcctggaatc atgtccgaaa
     1441 gcgatcgtgc cgccatcagg ttggcctttg cgggcaagac caagtttctt gaggccgttg
     1501 acaaactcta tacagagagc cagcagacgg ttgcgaaggc ggttggaacg aaagtcggaa
     1561 tggagaatcc aggttggggc atcgcggttt caaccggatc agcaacaaaa cctggttggc
     1621 tggtcgtcgg ttacaatggt catccggacg ttgctgaacc tgccgaagtg gagatcttct
     1681 acgttggttg tcct
//
LOCUS       DAOQUC010000995         1693 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_95087_length_1693_cov_3.990232, whole genome shotgun sequence.
ACCESSION   DAOQUC010000995 DAOQUC010000000
VERSION     DAOQUC010000995.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1693
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95087_length_1693_cov_3.990232"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            410..>1693
                     /locus_tag="PKH07_14885"
     CDS             410..>1693
                     /locus_tag="PKH07_14885"
                     /inference="COORDINATES: protein motif:HMM:NF017868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HNT36268.1"
                     /translation="MTDERKKRLFVYGTLTNPANVRTIAGRTFPSRRAILEGYEKISS
                     STFFPYIVRRSGSLVQGFLLEGLDQETMSLFDDFEGEGDLYRREKVTVIVGGEPVKAF
                     TYVGNKRNLLRYFDRGVAVEDRIKDHLEDRMETMLQERLSADDNKHVLRRAKAELYGS
                     TISDLVAAHFDHTGIPEYLVRKGLSDSVLPSLRFVASNAEALPYADNYVRFAVRHIVF
                     NQLEQRIRREFRGLVKAATAMYEYTISCLLTLKHLNAHKKRLKNLLQREGADNFDPQK
                     EYIDYAMAGVRAADALYSRREIADLVIRILSHRQEGRTPLGAEIEMSFLGVRAVHAKP
                     GDDPHFDGFHYSNDFDLPKRCWKLGGYIDDHSLYSPLTRRTRGFFEYAFGRLNLQGNI
                     AKPATQDPWILSELINEALRFAEIPPHSMHITLQPP"
BASE COUNT          402 a          432 c          451 g          408 t
ORIGIN      
        1 gtgctggctg tgcaacaagt actccatccc tgttgaccgg atagaagcga tacgtgaggg
       61 tgatccgaat cagtcggcag gaaagagact acaggcctac ttcgaccaag gtctgattca
      121 ggaacgtgtt cggaacatcc ttgcgaagat cgagaagcaa tcgtagaaat attgcgacga
      181 ctcgtcacgg ttttgatggc cattggatga ttgtcgtcag tgcgatcttg aatcgagcac
      241 gctcctggca agctttcgga agaggtagtc tgtctcgtga gcaagcacca catagaagcg
      301 ggaatagccc cagcgcctgc acgtgctcgc ctgctccgaa cgttccaagg atctttcggg
      361 ttccgaagtc ggcctgagac cgaactaagt ccttcttgag gtgtattgaa tgaccgacga
      421 gcgaaagaag cgtctattcg tctatggaac attgaccaat ccggccaatg tgagaaccat
      481 cgcaggaagg acgtttcctt ctcgaagagc tattcttgag ggctatgaga agatcagttc
      541 ctcaacgttt tttccctata tcgttcgccg ctctggcagc ctggttcagg gctttctcct
      601 tgaaggactg gatcaggaga cgatgagcct atttgatgat tttgagggag aaggagatct
      661 ctatcgccgt gaaaaagtaa ccgtcatcgt tgggggggag ccggtcaaag ccttcaccta
      721 tgtgggcaac aaacgcaatc tgcttcgcta ttttgatcgt ggagtcgccg tcgaagaccg
      781 aatcaaggac cacctggaag atcgcatgga gacaatgctc caggaacgct tgtctgccga
      841 cgacaacaag cacgttcttc gaagagccaa ggcagagctc tacggttcca ctatcagtga
      901 tttggtggcc gctcatttcg accatacggg tattcctgag tatctcgttc gcaagggact
      961 gagcgattca gtcttaccca gtcttcgttt tgttgcgtcc aatgcggagg cacttcccta
     1021 tgcagacaac tacgttcgat tcgccgtacg gcacattgtt ttcaaccaac ttgagcagcg
     1081 cattcgtcgg gagtttcgag gcttggtcaa agcggcgacc gctatgtacg aatacacgat
     1141 ttcctgcctt ctgacattga aacatcttaa cgcgcacaaa aagcggttga agaatctgct
     1201 ccagcgggaa ggtgctgata acttcgatcc tcaaaaagag tacattgact atgccatggc
     1261 gggtgtacgc gccgcagatg cactctacag caggcgtgaa atcgccgacc tggtcatccg
     1321 gatactgagc catcgtcagg agggacggac gccattgggt gcggaaattg agatgagttt
     1381 tctgggtgtt cgggcggttc acgccaaacc gggggatgac cctcatttcg atgggtttca
     1441 ttactccaat gactttgatc tgcccaagcg ctgctggaaa cttggcggct acattgacga
     1501 tcactcgctg tactctccgt tgactcgacg cacgaggggt ttctttgagt atgctttcgg
     1561 acggctcaat cttcagggca acattgccaa gccagcgacc caggatccct ggattctgag
     1621 cgagttaatc aatgaagcac tccgttttgc ggaaattcct ccccacagca tgcacatcac
     1681 tcttcaaccc ccg
//
LOCUS       DAOQUC010000996         1691 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_95282_length_1691_cov_3.405257, whole genome shotgun sequence.
ACCESSION   DAOQUC010000996 DAOQUC010000000
VERSION     DAOQUC010000996.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1691)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1691)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1691
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95282_length_1691_cov_3.405257"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="PKH07_14890"
     CDS             complement(<1..362)
                     /locus_tag="PKH07_14890"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNT36269.1"
                     /translation="MAEKILIGRTRRYACSGVWAAILVMLSVLNVGWLDPVRDQVEQA
                     NRKYLMGDHDGALKDYTQAQTERPEEPALHFNIGNVQVRKENLQEAAREYQQAATSTL
                     DRVLESQSLYNLGNVLAM"
     gene            complement(355..1362)
                     /locus_tag="PKH07_14895"
     CDS             complement(355..1362)
                     /locus_tag="PKH07_14895"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNT36270.1"
                     /translation="MGTSMVLNLLIVIPFLILFLRVTGRRRHEALERFGDSNLVLKLI
                     ANFSPERRRTKLALIVIAIALGIVALARPQFGETRQDVKRSGIDIIVALDTSKSMLAE
                     DIKPNRLSAAKRNTRGLIRRLEGDRVGIIAFAGTSFVLCPLTLDYGAAEMILEEMDTD
                     IVSMPGTNLEDAIQKARQSFVKGEQKYRVLVLITDGQATVGDPLKAAEEAFQEGIRIF
                     CIGLGQPSGIPIPVRDEKGNLKEYKKDSRGELVEATLDEETLMQIVSVSDGVYYRATS
                     SLDELDAIYDEVRAMEEKELKSQMYVQHIERFQWVLFPMLLLLGIEYLMPSVRRLVKN
                     G"
     gene            complement(1382..>1691)
                     /locus_tag="PKH07_14900"
     CDS             complement(1382..>1691)
                     /locus_tag="PKH07_14900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="aerotolerance regulator BatA"
                     /protein_id="HNT36271.1"
                     /translation="MGNAPYPVQDMFGRTHYQMVPVEIDEDLLKGISERTNGKYYRAT
                     DKSRLEEIFLDIERLEKTKLEAYGERRYRELFGWLLIPALLLILLELLLSETWLRVLP
                     "
BASE COUNT          392 a          484 c          402 g          413 t
ORIGIN      
        1 tgcatggcaa gaacattacc cagattatac aaggactgcg actccagcac tctatccaaa
       61 gtggaggtgg ccgcttgctg gtactcccgc gctgcttcct gaagattttc ctttcgcact
      121 tggacattgc cgatattgaa atgaagagca ggctcttccg gtcgttctgt ctgtgcctgg
      181 gtgtagtcct tcaacgcgcc gtcatgatcg cccataagat attttcgatt ggcctgctca
      241 acctgatcgc gcaccggatc aagccaacca acgttcaaca cagacagcat caccaggatg
      301 gcagcccaaa ccccgctgca ggcgtaccgt cgggttcttc cgatgaggat cttctcagcc
      361 attcttgacc aacctcctga ccgatggcat gagatattcg attcccagga gcaggagcat
      421 cggaaagagt acccattgga agcgttcgat atgctgaacg tacatctgcg atttaagttc
      481 cttctcttcc atagcacgca cttcgtcgta gatcgcatcc agttcatcta acgaactggt
      541 tgcgcggtag tatacaccat cgctgacgga aacgatctgc atgagtgttt cctcatccag
      601 agttgcctcg accagttccc ctctggagtc cttcttgtat tccttcagat tgcctttttc
      661 atctcgaact ggaatgggaa tacctgacgg ctgacccagt ccgatgcaga aaatccgaat
      721 cccctcctgg aatgcttctt cggcagcctt gaggggatct ccgacagttg cctgtccatc
      781 cgtgatcaag acaaggaccc ggtatttctg ttctcccttg acgaaactct gtcgagcttt
      841 ttggatggcg tcttccaggt tggtgcccgg catggacaca atgtcggtat ccatctcttc
      901 caggatcatc tctgcagcgc cgtagtcaag agtcagagga cacagcacga aactagttcc
      961 cgcaaaggcg atgatgccaa cgcggtcgcc ttccaggcgt cgtatcagtc cacgtgtgtt
     1021 tcgctttgcg gcggacagcc gattcggttt gatatcttcg gcaagcatgc tcttggacgt
     1081 atccagagcc acgatgatat caatgccgga gcgcttgaca tcctggcggg tttctccaaa
     1141 ctgcggacgc gccagcgcca caatccccaa ggcgatggca atgacaatca gtgccagttt
     1201 tgtgcgtcgt cgctccgggc tgaaattcgc aatcagtttc agcacaagat tcgagtcccc
     1261 gaaccgctcc aaggcttcat ggcggcggcg tcccgtgacc ctcagaaaaa ggatcaaaaa
     1321 tgggatcaca atgagcagat tcaaaaccat tgatgtaccc aaccgcacag ccgtcacctc
     1381 tctatggtaa gacccggagc caggtttccg agagcagcag ttcgagcaaa atcaggagta
     1441 gtgcaggaat aagcagccat ccgaacaact cccggtaccg acgctctcca taagcttcca
     1501 acttggtctt ttcaagacgt tctatgtcta agaagatttc ctccaacctt gatttgtccg
     1561 tggcccggta atacttgcca ttggttctct cggaaatccc cttcaggaga tcttcgtcaa
     1621 tctcaaccgg aaccatctga tagtgggttc ttccaaacat atcctgaacc ggataaggtg
     1681 cattgcccat g
//
LOCUS       DAOQUC010000997         1689 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_95484_length_1689_cov_3.061200, whole genome shotgun sequence.
ACCESSION   DAOQUC010000997 DAOQUC010000000
VERSION     DAOQUC010000997.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1689
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95484_length_1689_cov_3.061200"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1101
                     /locus_tag="PKH07_14905"
     CDS             <1..1101
                     /locus_tag="PKH07_14905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014042337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HNT36272.1"
                     /translation="RDNYPFGMFAVPMEDVVRIHSSSGTTGKPTVVGYTRNDIQVWAE
                     VMARTIGCAGGDKTSVVQVTYGYGLFTGGLGVHYGSELMGCTTIPISGGNSKRQVMIM
                     EDFGTTILACTPSYALALAEVLVEEKIDRSKLKLRAGIFGAEPWTNEMRDELEKRWGI
                     LALDIYGLSEIIGPGVSSECDRKCGLHINEDHFIPEIIDPETGNVLPDGELGELVFTC
                     VTKEAFPVIRYRTRDLSRLFREPCACGRTLTRMERVSGRTDDMLIIRGVNVFPSQIES
                     VLVKVEGAEPHYLIIVDRVGALDDIEVQVELSDSAFSDEVRKIEDLRTRIAGELYGVL
                     GLHAKVKLVEPKTIERSMGKARRVIDKRRGDA"
     gene            1145..1576
                     /locus_tag="PKH07_14910"
     CDS             1145..1576
                     /locus_tag="PKH07_14910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HNT36273.1"
                     /translation="MKLKQISIFMENRKGRLAEVARVLGNANINIRALSLADTSDFGI
                     LRLIVNEVDTAFKVLKDNGFTVRDTEVVAVEVEDHPGGLAEVLAVLAKEDLNVEYMYA
                     FVEKSENKAVLVFRIEELTKAINTLQKAGIRILTGEQIYKM"
BASE COUNT          414 a          359 c          511 g          405 t
ORIGIN      
        1 cgcgacaact atcccttcgg catgtttgct gttcccatgg aagatgttgt tcgcatccat
       61 tcttcgtcgg ggactacggg caaaccgaca gttgtaggct acactcgaaa cgacattcaa
      121 gtgtgggctg aagtgatggc gcgaaccatt ggctgtgcag gtggggacaa gaccagtgtg
      181 gttcaggtga cctacggata cggtcttttc acaggaggtc tgggggttca ctatggttct
      241 gaactcatgg gatgtaccac aatcccgatc tcaggtggaa acagcaagcg tcaggtcatg
      301 attatggagg acttcggcac caccattctg gcgtgtactc cgtcttacgc tctcgccctc
      361 gcggaagtgc tggtcgagga gaagattgac cgttcgaaac tgaagttaag ggcaggcatc
      421 ttcggcgcag agccttggac aaacgagatg cgggatgaac tggagaagcg ttggggtatc
      481 ctcgctctgg atatctacgg tctgagtgag atcatcgggc ctggcgtttc cagcgaatgt
      541 gatcggaaat gcggtctgca catcaacgaa gatcatttca ttccggagat cattgacccg
      601 gagacgggaa atgttctgcc ggatggagaa ctgggagaac tggtctttac ctgtgtgacc
      661 aaggaagcct ttccggtgat ccgatacaga acgcgtgatc tatcgcgtct cttccgagag
      721 ccctgtgcct gcggtcgaac gctgactcga atggagcgtg tgagcggtcg tacagacgac
      781 atgttgatca tccgtggagt gaacgtgttc ccgtcgcaga ttgagagtgt tctggtcaaa
      841 gtcgagggag ccgaaccgca ctaccttatc attgtagatc gggttggtgc tttggatgat
      901 atcgaagtgc aggtagagct gagtgactcc gccttctcgg acgaggtgcg gaagattgaa
      961 gacttgagaa ctcgaatcgc gggtgagttg tatggagttc tcggacttca tgccaaggta
     1021 aaacttgtgg agccaaagac cattgaaaga agtatgggca aagccaggcg tgttatagac
     1081 aagcgcagag gtgatgcata gagagttgtt tgcagagaac ctgttcactc aggagggtga
     1141 aacgatgaaa ctgaagcaga tatcaatctt catggaaaat cgaaagggac gcttggcaga
     1201 ggttgccaga gtcctgggga atgccaacat caacattcga gccctatcct tggctgacac
     1261 atctgatttt ggcattcttc gcctgatcgt caacgaggta gacaccgctt tcaaagtgct
     1321 taaagacaac ggcttcactg ttcgggatac cgaagttgtt gctgttgaag tggaagacca
     1381 ccccggtggc ttggcggaag ttctggcggt gctcgccaaa gaagacttga acgtggaata
     1441 catgtatgcc ttcgtggaga agtccgagaa caaggctgtg ttggtatttc ggattgaaga
     1501 actcaccaag gcgataaaca ctcttcagaa ggcaggcatc cgtattctaa ctggtgaaca
     1561 gatatacaag atgtagtggt ttgcatgaac agtgaaatgc gatggagtgc tgagtctctg
     1621 cgactgggca ggttgtttga gactggtctg ggggacactg aggtaatggg tgtttttgtt
     1681 cgaaggaag
//
LOCUS       DAOQUC010000998         1688 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_95614_length_1688_cov_2.135946, whole genome shotgun sequence.
ACCESSION   DAOQUC010000998 DAOQUC010000000
VERSION     DAOQUC010000998.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1688
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95614_length_1688_cov_2.135946"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..406)
                     /gene="trpA"
                     /locus_tag="PKH07_14915"
     CDS             complement(<1..406)
                     /gene="trpA"
                     /locus_tag="PKH07_14915"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HNT36274.1"
                     /translation="MSRISRTFANLSEKSQKALIAYIMAGDPSLDATYPAVKALRSAG
                     VDIIELGFPFSDPLADGPTIQAAAYRALQNPIGLDDYFRLIQRVRAEDDVPLILMTYY
                     NVIYHYGLEAFAKTAGQAGLDGIIIPDLPLEES"
     gene            complement(403..1605)
                     /gene="trpB"
                     /locus_tag="PKH07_14920"
     CDS             complement(403..1605)
                     /gene="trpB"
                     /locus_tag="PKH07_14920"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009541721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HNT36275.1"
                     /translation="MANSLPDENGHFGIYGGRFVPETLMAALEELASVYAETLNDQDF
                     HRQLDYYLTNYAGRPSPLYFAQRLTEHCGGANIYLKREDLNHTGAHKINNTLGQILLA
                     HRMRKPRIIAETGAGQHGVATATAAALFGLECVVYMGEEDIERQRLNVFRMELLGATV
                     NPVTSGSRTLKDAMNEAIRDWVTNVRTTHYIIGSTAGPHPYPMIVRDFQAVIGKEARE
                     QILKQEGRLPDALVACVGGGSNAMGLFYPFFDDENVLFFGVEAAGHGLETGKHSASLC
                     KGTVGVLHGSKSYLLQDKNGQIDIAHSISAGLDYPGVGPEHSYYKDSGRAQYVGITDD
                     QALDAFSLLSKLEGIIPALESSHAVAYACDLARKLGSGKTIIVNLSGRGDKDVYTVAK
                     AMGKEIVL"
BASE COUNT          404 a          441 c          449 g          394 t
ORIGIN      
        1 cggactcctc gagtggcaag tcgggaatga tgatgccgtc gagccccgct tgacctgcag
       61 tcttggcaaa cgcttcaaga ccgtagtgat agatgacgtt gtaataagtc atgagaatca
      121 gagggacatc atcttctgct ctgacacgct gaatcaacct gaaatagtcg tccagtccaa
      181 tcggattctg aagagcacgg taggcagccg cctgaatcgt tggtccgtcg gccagtgggt
      241 cagagaaggg aaatccaagt tcgatgatgt ccacaccagc agatcgaagc gccttgaccg
      301 caggatatgt ggcatccagg gacggatcgc ctgccatgat gtatgcaatg agggcttttt
      361 gactcttctc tgagagattc gcaaaggttc tggagattcg gctcatagca caatctcctt
      421 tcccatggct ttggcaaccg tgtagacgtc cttgtccccg cggcctgaca ggttgacgat
      481 gatcgtcttc ccggaaccaa gttttcgcgc aaggtcgcag gcataggcga cagcgtgcga
      541 actttcaagg gcagggatga ttccctcaag tttggacaac agcgagaacg catcgagtgc
      601 ctgatcatcg gtaataccca catactgcgc tctgccggaa tccttgtagt aggaatgctc
      661 aggacctact cccggatagt cgagacctgc agagatggaa tgtgcaatgt caatttgtcc
      721 gttcttgtcc tgcagaaggt atgacttgct gccatgaaga acaccaaccg tgcctttgca
      781 caaactggcg gaatgctttc ctgtttcaag tccatgtcca gcagcctcga cgccaaaaaa
      841 caggacattc tcgtcgtcaa aaaacgggta gaagagaccc attgcgttgc ttccaccacc
      901 gacacacgcg accaatgcgt cagggagtct gccttcctgt ttcagaatct gttcccgggc
      961 ttcctttcca atgaccgcct gaaaatctct gacaatcatt ggatagggat gaggcccggc
     1021 ggtagagcca atgatgtagt gtgtggtgcg tacgttggtg acccagtcgc gaatggcctc
     1081 gttcatcgca tccttgagtg ttcgagaacc agaggttaca ggattcactg tggctccaag
     1141 caactccatc cgaaagacgt tgagcctttg cctctcgata tcttcttctc ccatgtagac
     1201 aacgcattca aggccaaaga gcgccgccgc tgttgcggtg gcgacaccat gttggccggc
     1261 acccgtctcg gcgataatac gaggtttgcg cattctgtgg gcaaggagaa tctgtcccag
     1321 agtgttgttg atcttgtgag cacctgtgtg gttcagatct tcacgcttga ggtagatatt
     1381 cgcgccaccg cagtgctctg tcagtctctg ggcgaagtag aggggactcg gtctcccagc
     1441 gtagttggta agatagtagt ccagctgccg atggaaatcc tgatcattga gagtctcggc
     1501 atagacggag gccaactcct caagagcagc catgagcgtt tcgggtacaa aacgcccacc
     1561 gtagatgcca aaatgaccgt tctcgtcggg caatgaattt gccatagttc ctaatatcct
     1621 tgccaagata actcgacgcc gacaggcaac aaataaaccc atgacatgac catgtatgga
     1681 tggacatg
//
LOCUS       DAOQUC010000999         1688 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_95625_length_1688_cov_1.859155, whole genome shotgun sequence.
ACCESSION   DAOQUC010000999 DAOQUC010000000
VERSION     DAOQUC010000999.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1688
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95625_length_1688_cov_1.859155"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(67..>1688)
                     /locus_tag="PKH07_14925"
     CDS             complement(67..>1688)
                     /locus_tag="PKH07_14925"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1854 domain-containing protein"
                     /protein_id="HNT36276.1"
                     /translation="IMAIIFQFGGWIVWFVGGRDVLHGQMTLGSLMAFLGYLWMFYGP
                     LGHLTQFTNWLTQFATQAHRIFEILDTPVEITDPKQPISIEPVKGKIEFRRVTFGYSR
                     HTPILRDLDLVIEPGEMIGIVGRSGSGKTTIVNLICRFYDVDEGSVLIDDADVRDIRK
                     EELRSLAGVVLQEPFLFRGSIWENLTYGKPEASVEEVLLASKEGNSHDFIMRSAYGYD
                     TWVGERGAGLSGGERQRVSIARVLLTDPRILILDEATSSVDAESEASIQAALAEVVKG
                     RTTIAIAHRLSTLRNANRILVVDHGVIVESGRHDELVANDGLYAKLVRIQGQVSAPTI
                     QQLAVQSEEDEEKEKAEERERERAEAKKTGPLPDISSHHPRWLTPEVAHIHIGRLGAL
                     HVSIQGEEIYGGVFAVRCLPISHPWEFTSLRHFNEDKRETEIGLIRNLKDWPEEIQNL
                     IRQSLGKRYFVHTIRSIESIEQIQGYLNFKVETDLGPMEFMLRWQGDRAHDYGPSGKM
                     LIDTEENRYVIADVEALAESERALFLRFIYW"
BASE COUNT          391 a          487 c          415 g          395 t
ORIGIN      
        1 ggcctgagca ggcagcagtc tttgtgaaat cacgccggga gaatcggttg tccatagcgt
       61 cctccgttac cagtagatga aacgcagaaa cagcgcccgt tccgactctg caagcgcctc
      121 cacatctgcg atgacatatc ggttctcttc ggtgtctatt agcattttgc ccgaaggccc
      181 atagtcgtgg gcacgatccc cctgccagcg cagcatgaac tccattggtc ccaagtcggt
      241 ttccaccttg aaattcagat agccctgaat ctgttcgatg ctctcgatgg agcgaatcgt
      301 atgaacgaaa tacctcttgc caagcgattg ccggatgagg ttctgaatct cctcaggcca
      361 atctttcaga ttccggataa ggccaatttc agtctcccgt ttatcctcat tgaaatgacg
      421 cagagaagtg aactcccagg gatgactaat cggcagacaa cgcacagcaa agactccgcc
      481 gtaaatctcc tctccctgga ttgagacatg cagtgctccc agtcttccaa tgtgaatatg
      541 agcgacctcg ggcgttaacc accttggatg gtggctggag atatctggaa gaggcccggt
      601 cttctttgcc tcagctctct cgcgttctct ttcttctgcc ttttcttttt cttcgtcctc
      661 ttcagactga acagccaatt gctgaattgt cggtgcagag acctgtcctt gaatgcggac
      721 aagttttgcg tacaggccat cgttggcaac aagttcatca tgccttcctg attcaacgat
      781 gactccgtgg tcaacgacca gaattctgtt ggcgttgcga agcgtgctca gccgatgcgc
      841 gatggcaatg gtcgttcgcc ccttgaccac ttccgccagt gccgcttgga ttgaggcttc
      901 actctcagca tcaacgctgc tggttgcctc atccaagatg aggatcctgg gatcggtcaa
      961 gagcacacga gcgatgctca cgcgttgtct ttcaccgccg gatagacccg ccccacgctc
     1021 gcctacccac gtatcgtaac cataggcgga gcgcatgatg aagtcatggg aattcccttc
     1081 ctttgaagca agcaatactt cttcaaccga agcctcgggc ttgccatagg tcaggttttc
     1141 ccagatgctc ccacgaaaaa ggaaaggttc ctgcaggaca actccggcga ggctcctgag
     1201 ctcctccttt cggatatccc gaacgtcggc atcatcaatg agcaccgaac cctcatccac
     1261 atcatagaaa cggcagatca agtttacgat ggtcgtcttt ccagagccgc tcctgcccac
     1321 aatgccaatc atctccccag gctcaatgac caagtccaag tccctcagaa tgggggtatg
     1381 gcggctgtaa ccaaaagtga cacgcctgaa ttcaatcttg cccttaaccg gctcaatgct
     1441 gataggctgc ttcggatctg taatctcgac aggagtgtcg agaatctcaa agatgcgatg
     1501 tgcttgtgtg gcaaactgcg tgagccagtt ggtgaactgg gtcaagtgtc caagcgggcc
     1561 atagaacatc cacagatagc cgagaaaggc catcagactg cccagggtca tctggccgtg
     1621 gagcacatcg cggccgccga cgaaccagac gatccagccg ccgaactgga aaatgatcgc
     1681 catgatag
//
LOCUS       DAOQUC010001000         1686 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_95769_length_1686_cov_3.530963, whole genome shotgun sequence.
ACCESSION   DAOQUC010001000 DAOQUC010000000
VERSION     DAOQUC010001000.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1686
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95769_length_1686_cov_3.530963"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..393)
                     /locus_tag="PKH07_14930"
     CDS             complement(<1..393)
                     /locus_tag="PKH07_14930"
                     /inference="COORDINATES: protein motif:HMM:NF015412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HNT36277.1"
                     /translation="MAVNIGLVGFGTVGKGFVRALGMNRELIERHAGTELRLLRIADL
                     DIDRDRGVDLCGAELCRDAFSVLEDPNLDIIIELIGGVDLSRKFILTALRNGKHVVTA
                     NKALLAEHGDELFGAADQAGKAIYFEGAV"
     gene            complement(431..1351)
                     /locus_tag="PKH07_14935"
     CDS             complement(431..1351)
                     /locus_tag="PKH07_14935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HNT36278.1"
                     /translation="MSLVVVGSVALDSVETPYGKRDEALGGAATYASCAASLFGEVAM
                     VAVVGSDFPKAYHDKLLQRGICLDGLVVLEGPTFRWTGVYQGDMGVVRTLNTQLGVFE
                     SFRPIIPDSLKRPRVVLLESIHPELQLEVLDQMERPELVAMDTIRLWINQKPDKVREV
                     IRRSDLVILNDEEVRSITGENRLVRAAETILSWGPRNVIVKRGEHGASLYGEVGPFSV
                     PSFPTPQVKDPTGAGDSFAGAFLGFLLRGGNLRSAQRIRQAMVVGTVVASFNVEELSI
                     DRLERVTLQEIQERCRFIRESTAFDEVALG"
BASE COUNT          405 a          516 c          409 g          356 t
ORIGIN      
        1 caccgctccc tcgaagtaga tagcctttcc ggcctgatcc gcggcgccga acagctcatc
       61 gccatgctcg gccaggagag ctttgttcgc ggtgacgacg tgtttgccat tcctgagcgc
      121 ggtcaggatg aacttgcggg acaaatccac gccgccgatg agttcaataa tgatatcgag
      181 attgggatct tccaggacag agaaagcatc gcgacagagt tccgcaccac acaaatccac
      241 accgcggtct cggtctatat ccagatcggc gattcggagc agtctcaact cagttcccgc
      301 gtgacgctcg atcagttcgc ggttcatgcc cagagcccga acaaaaccct tgcccacagt
      361 tccaaatccg acaagcccaa tatttaccgc catgttgttg attcccttct gcgtgagagc
      421 aggaacaccc tcagccgagc gccacctcat caaacgccgt gctttcacgg atgaacctgc
      481 aacgctcctg gatttcctgg agcgttaccc gctccaggcg atcaatgctc agttcctcca
      541 cattgaacga agcgaccacc gttccgacca ccatggcttg tcggattcgt tgtgcggagc
      601 gcaagttgcc accccggaga aggaatccga ggaaagcgcc agcgaaactg tctcccgctc
      661 ctgtcggatc cttcacttga ggcgttggaa aagagggcac tgaaaacggt ccgacttctc
      721 cgtaaagcga cgctccgtgt tcgccacgtt tgacgataac atttcgaggt ccccaactca
      781 atatcgtctc tgcggctcgc accagacggt tctcgccggt aatacttcgg acttcttcat
      841 cattgagaat caccaagtcc gaacgccgaa tcacttcgcg aactttgtcc ggtttctgat
      901 tgatccagag gcggattgtg tccattgcga ccagttcggg gcgctccatc tgatccagca
      961 cttccaactg gagttcagga tgaatgcttt ccagaagaac gacccgtgga cgtttgagac
     1021 tgtcggggat gattgggcgg aaggattcga acacacccaa ctgggtgttg agcgttctga
     1081 caacccccat gtcaccttga tagacacccg tccatcgaaa cgtaggtcct tcaagaacga
     1141 caagtccatc caaacagatc cctctttgaa ggagtttgtc gtgataggcc tttggaaaat
     1201 cgcttcccac aacggcgacc atggccacct ctccaaagag actcgctgca caggaagcgt
     1261 acgttgccgc accaccaagt gcttcgtcac gcttgccgta aggggtctcc acactgtcca
     1321 aagcaaccga acccacaacc accagactca tcaacagaac tccttcctga aaaccagtca
     1381 tcccaacaaa ccaagacagt aacacaggga attggatcta cagccagcca ctgccttgct
     1441 tggtgtcttt ataccacact cggcggagtt gaatccagtc ctctctcccg tttcgagcgt
     1501 tgcagccctc aggttcacga agcggctctg atggccgccc ccagaggagt cagtcggagt
     1561 gcgggaaaag agagtgcacc cggcaagcct cagtcgttcg gaatgacagc ctccgccctt
     1621 ctgtaactga gccaacgata cgtccttcac catagaaaca gttgacaaga aatgactggt
     1681 gagtta
//
LOCUS       DAOQUC010001001         1686 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_95797_length_1686_cov_2.930104, whole genome shotgun sequence.
ACCESSION   DAOQUC010001001 DAOQUC010000000
VERSION     DAOQUC010001001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1686
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95797_length_1686_cov_2.930104"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /locus_tag="PKH07_14940"
     CDS             complement(<1..254)
                     /locus_tag="PKH07_14940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015469496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HNT36279.1"
                     /translation="MNVTDDLMKQLSRLQGVFTDIDDTLSTNGKLTSEAFASLWRLHE
                     AQIRIVIVTGRSAGWCDHIARFWPVDAVVGENGGFYFHHDG"
     gene            463..1197
                     /gene="thyX"
                     /locus_tag="PKH07_14945"
     CDS             463..1197
                     /gene="thyX"
                     /locus_tag="PKH07_14945"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013431906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HNT36280.1"
                     /translation="MGRVRSNVTLIRYTEDPEQVVASAARICYAADSEKAFDELPEKA
                     ADFVRMLRRLGHLSPYEHAVFTFYVEGVSRAMSHQMVRHRLASYSQRSQRYVEHNAFD
                     YVVPPQLEGKEVFLDGKSVNAVDYFCETMDWIAERYRVLNEALGRSGESSNEDARYLL
                     PNACETKLVVTMNARELLHFFEERLCQRAQWEIRDVAERMLELVRKVAPSLFEKSGPK
                     CLRYNRCTEGKMTCGQFDAVKQRYGV"
     gene            complement(1200..>1686)
                     /locus_tag="PKH07_14950"
     CDS             complement(1200..>1686)
                     /locus_tag="PKH07_14950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase M14"
                     /protein_id="HNT36281.1"
                     /translation="RDYLGEPADSIYPEVRELREFVPKWSGGLLRVGLDLHCPYIRGA
                     HNEVIYLVGSRSEQIAQQQVLFGQLLESLAQGALPFRAEDYLPYGKAWNTAANYGSGR
                     SFGNWLQDLSEVRLASTIEIPYARARQVEVNQETAQAFGQDLGRAISAYLRSDPELVS
                     K"
BASE COUNT          414 a          415 c          461 g          396 t
ORIGIN      
        1 ccgtcatggt ggaaataaaa accaccgttc tctccaacga cagcatcaac aggccagaat
       61 cgggcgatat gatcacacca gccggcggat ctgcccgtga cgattacgat tcggatttgt
      121 gcctcgtgaa gcctccagag cgacgcgaac gcctcagatg tcagtttccc gttggttgac
      181 agggtatcat caatatcggt gaatacgccc tgcaaacggg acagctgttt catcaaatca
      241 tcggtgacgt tcatctgctt cctcgaatca aacgcaaact cgcgtgaaca taacacacaa
      301 cgattctgga atccaagtgc aggctgtgat actcttcaga ccgtgaacac cggtactcta
      361 cgagcagaaa agtgtgcggg atgtatttac atccgcaagt ctcttgtctg tgaaggaaac
      421 acgcttcgct ggagtgatac ctcttaagag agcaggaatt cgatgggaag agttcggtca
      481 aatgttacgt tgattcgtta tacggaagat cccgaacagg tggtagcctc tgctgcgaga
      541 atctgctacg cggccgattc ggaaaaggct tttgacgagt tgccagagaa ggcggcggac
      601 tttgtgcgga tgcttcggag gttggggcat ctcagcccat acgaacacgc agtctttacg
      661 ttctatgtgg aaggagtgtc gcgggctatg tcccaccaga tggttcggca taggcttgcc
      721 agctattctc aacgaagcca gcgctatgtt gaacacaatg cttttgacta tgtcgttccg
      781 cctcaactgg aagggaaaga ggtcttcttg gatggcaaga gtgtgaacgc ggtggattac
      841 ttttgtgaga cgatggactg gattgccgag aggtatcgag ttctgaatga ggcattagga
      901 cgcagtggcg agagcagcaa tgaagatgcg cgatacctgc ttccgaatgc ctgcgaaacg
      961 aaactggttg ttaccatgaa cgcgcgggaa ctgttgcatt tttttgaaga acgtctgtgt
     1021 caacgtgctc agtgggaaat tcgggatgtt gccgagagaa tgcttgaact tgtcagaaaa
     1081 gttgctccgt cgctttttga gaagtccggc ccaaaatgtc ttcgatataa ccgatgtacc
     1141 gaaggcaaga tgacttgcgg gcagttcgac gccgtgaagc aacggtacgg cgtctgatac
     1201 tatttgctca cgagttctgg atcacttcgg agataggctg aaatcgcccg acccaaatcc
     1261 tgtccaaacg cctgggctgt ctcctgattt acttcgactt gacgagcacg tgcataggga
     1321 atctcaatgg tgctggcaag tctcacttcg ctgagatcct gaagccaatt gccaaaactt
     1381 ctgcctgagc cgtagttggc agcagtgttc cacgctttcc cataggggag gtaatcctcc
     1441 gctcggaatg gcagagcccc ttgtgccagg gactcgagca actgcccgaa aagaacctgc
     1501 tgttgtgcga tctgttcaga ccgtgatcca acaaggtaga tgacttcatt gtgagcgccc
     1561 cgaatgtaag gacagtgaag atccagtccg acacggagca gaccgcctga ccactttggg
     1621 acgaactccc tcagttcgcg cacttccgga tagatgctgt cagcaggctc acccaagtag
     1681 tccctg
//
LOCUS       DAOQUC010001002         1685 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_95897_length_1685_cov_3.161350, whole genome shotgun sequence.
ACCESSION   DAOQUC010001002 DAOQUC010000000
VERSION     DAOQUC010001002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1685
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95897_length_1685_cov_3.161350"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..341
                     /locus_tag="PKH07_14955"
     CDS             <1..341
                     /locus_tag="PKH07_14955"
                     /inference="COORDINATES: protein motif:HMM:NF014072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF111 family protein"
                     /protein_id="HNT36282.1"
                     /translation="DAFLTPVIMKKSRPAVQLTVIAAPDNRLALTKTIMEETTTFGLR
                     WSVLDREVLQREIRSVETPYGAVQVKFGSGIAQKAVPEYHDCLRVAREKSVPLQKVYQ
                     AAQRASEPQD"
     gene            372..1412
                     /locus_tag="PKH07_14960"
     CDS             372..1412
                     /locus_tag="PKH07_14960"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HNT36283.1"
                     /translation="MKNKKMIIGIVVTLIGLGLFVRQAAQQEDWGAIGRAFLQARYWL
                     LIPSLIVLFSSLLFRAYRWRVFLHGHGEYGVGELFAFTSIGFMGNSIFPARAGEIMRC
                     ILLAEKKKVSFMTLLATVVVERLFDLVVVIGFFAVTLLLIPLHEGVTAETSKLIRLSG
                     QVAITGFLVILVVLLAMTFKPQLVKTILSKTIGGILPAPLMGKILSLMDSLVEGLSCL
                     RSVRQIFWSLFWSVLVWLAIGFSEYLIFIAFTDLLGLKESFFVMALIAFAVAAPSTPG
                     YLGVYDVVCQSILIGMFLVDVATATSSTLILHASQLFPIILLGFVCLWWQGLSFRQLR
                     DTEKRAELKQAD"
     gene            1437..>1685
                     /locus_tag="PKH07_14965"
     CDS             1437..>1685
                     /locus_tag="PKH07_14965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36284.1"
                     /translation="MGDALVLKDVFGRTVRLSDERFSHILEHPEMAGLSKEIERVLRM
                     PKVVRRSRSDSSVLLFYEFLTATIFGDKWLCVVVKYQES"
BASE COUNT          341 a          416 c          473 g          455 t
ORIGIN      
        1 tcgatgcctt cctgacaccg gtgatcatga aaaagagccg accggctgtt caactgaccg
       61 tgatcgccgc accggacaat cgcctggctc tgaccaaaac catcatggaa gaaaccacaa
      121 cgttcggact tcgatggtcc gtcttggatc gggaagtact ccagagggag attcgatccg
      181 ttgaaacgcc ctatggtgcc gttcaggtca agttcggctc cggcatcgcg cagaaggcgg
      241 ttcctgaata tcacgactgc cttcgggtgg ctcgggaaaa gtccgttccg cttcagaaag
      301 tctatcaggc tgcacagaga gccagtgaac cacaggactg atactgtccg ccgacaggag
      361 aaacagacac gatgaagaac aagaaaatga tcatcggaat tgttgtgacg cttatcggac
      421 tggggctttt cgtccgacag gcggcacagc aggaagactg gggcgctatt ggcagagcgt
      481 ttctccaagc gcggtactgg cttctgattc cttctctgat tgtgcttttc tcaagcctgt
      541 tgtttcgtgc gtaccggtgg cgggtcttct tgcatggcca cggggagtac ggtgtggggg
      601 aactctttgc gttcacttcc atcggattca tgggcaattc gatcttcccc gcacgtgcag
      661 gagagattat gcgctgcatc cttttggcag agaagaagaa agtttccttc atgaccctgt
      721 tggcgaccgt tgttgtggaa cggctttttg atctggtggt tgtcatcgga ttctttgctg
      781 tcacactgct cctgattccg cttcatgagg gggtgaccgc agagacgtca aaactgatcc
      841 gcttgtccgg acaggttgcc atcacgggat ttctggtcat cctcgttgtg cttctggcga
      901 tgacgttcaa gcctcagttg gtcaagacaa ttctgtcgaa aacaatcggc ggaattcttc
      961 ctgcgccatt gatgggaaag attctcagtc tgatggattc actcgttgag ggactctcct
     1021 gtctgagaag tgttcgacag attttctggt ctttgttttg gtcggtgttg gtctggctcg
     1081 ccatcggctt ctccgagtat ctcatcttca tcgccttcac cgatctactc ggtctgaagg
     1141 aatcgttctt tgtcatggcg ttgatcgctt ttgccgtagc agcgccaagc actcccggat
     1201 atctgggcgt ctacgatgtg gtctgtcaga gcatccttat cggcatgttt cttgtggatg
     1261 tggcaactgc gaccagcagc accttgatcc tgcacgcctc acaacttttt cccatcatcc
     1321 tcttgggatt cgtctgcctt tggtggcagg gactttcctt ccgtcagctt cgggatactg
     1381 agaagcgggc tgagttgaag caggcagact agacgaatgc ggaggcactt gggaatatgg
     1441 gagatgctct ggtcctcaag gacgtctttg gcagaactgt acggctttcc gatgagcgat
     1501 tttctcatat cttggaacac ccggagatgg cgggactgag taaggagatt gagcgcgtac
     1561 tgaggatgcc caaggtcgtt cgtcggtcgc gaagcgattc ttccgttctg ctcttctatg
     1621 agtttctgac agcgacgatc tttggtgaca aatggctctg tgttgtggtt aaatatcaag
     1681 agagt
//
LOCUS       DAOQUC010001003         1681 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_96283_length_1681_cov_2.742312, whole genome shotgun sequence.
ACCESSION   DAOQUC010001003 DAOQUC010000000
VERSION     DAOQUC010001003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1681
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96283_length_1681_cov_2.742312"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..256
                     /locus_tag="PKH07_14970"
     CDS             <1..256
                     /locus_tag="PKH07_14970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36285.1"
                     /translation="RGRSSLWLLVCDKQNIRREIGWEYGKYCSPIYEGDIDFKKVVAI
                     LQAAEYFGDYCIENESLSRFPQEERGAILKKEAEFLRGLV"
     gene            418..1206
                     /locus_tag="PKH07_14975"
     CDS             418..1206
                     /locus_tag="PKH07_14975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020249217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HNT36286.1"
                     /translation="MGCMNEDYPRTLLELEERFSVESACRAYLYALRWPMGFLCPVCG
                     ASEAWAMDRGLWLCRGCRHQVSVMAGTIFQDTHLPLTTWFRAMWYVTSQKNGVSALGL
                     QRILGLGSYKTAWALLHKLRRAMVRPGRDRLRGIEEVTARSLHDFIADAVEPRSTVRT
                     DGLNAYLGLQSYEHDRPIQRDHGPKARLLPRVHQVISLLKRWLLGTHQGAIGHDHLAY
                     YLDEFTFRFNQRKSKSRGKLFYRLVQQAVQVGPVPFESLTNHNP"
     gene            1469..>1681
                     /locus_tag="PKH07_14980"
     CDS             1469..>1681
                     /locus_tag="PKH07_14980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36287.1"
                     /translation="MMQQYLEMKEQCGDAILLFRCGDFYETFGEDARIVSKALNIALT
                     TRDRNTDPTPLAGFPHHALDQYLPKLV"
BASE COUNT          359 a          405 c          520 g          397 t
ORIGIN      
        1 tcgcggacgc tcatcgctct ggctccttgt atgcgacaaa cagaatatcc ggcgagagat
       61 cggatgggaa tacgggaaat attgcagtcc catctacgaa ggagatatcg atttcaagaa
      121 ggtggttgcg attcttcagg cagcagagta tttcggagac tattgcatcg agaacgaatc
      181 cctgagccgt ttcccgcagg aagagcgcgg tgcgattctg aagaaggaag cggaattcct
      241 tcggggactg gtctgaggaa tgcttgagcg acagaccggc gggacgggtg atttgtatca
      301 agaacgcatg gcgtctcgcg tgatagagca aggaatgtgg agtaggtcaa cccccacctg
      361 acccaatgtc attcagttac ggatatttac ttgactcaac cgaccccaac ctgtagtatg
      421 gggtgtatga atgaggatta tccccggacg cttctggaac tggaagagcg gttttcggtg
      481 gaaagcgctt gccgggcgta tttgtatgcg ttgcggtggc cgatgggatt cctctgcccg
      541 gtctgcggtg ccagtgaggc ttgggcaatg gatcgagggt tgtggctttg ccgtggctgc
      601 cgccatcagg tttcggtgat ggcgggtacg atcttccagg acacccactt gccgttgacg
      661 acttggtttc gagcaatgtg gtatgtgacg agtcagaaga atggggtgag cgctttggga
      721 ttgcagcgga tcctggggct gggcagttat aagacggctt gggcgttgtt gcacaagttg
      781 cggcgggcga tggtgcgtcc gggacgggac cgtttgcggg ggattgagga ggtcaccgcg
      841 aggagtttgc acgattttat tgcggatgcg gtggaacccc gcagtacggt tcgaaccgac
      901 ggcctgaacg cttatctggg cctgcaaagc tatgagcatg atcgtccgat ccagcgcgac
      961 cacggaccca aggcacgcct gttgccgaga gttcatcagg tgatatcgct gctgaaacgc
     1021 tggttgttgg ggactcatca gggggcgatc ggtcacgacc acctggctta ctaccttgac
     1081 gaattcacgt ttcggtttaa ccagcgcaag tccaaatcgc gcggcaagtt gttctaccgt
     1141 ttggttcaac aggccgttca ggtcggtccg gtcccgtttg agtctctcac aaaccacaac
     1201 ccctaggggt cggttgagtc aagtaaatat ccagatcact taatgacatt gggtcaggtg
     1261 ggggttgata agcctggcga attcaatttt ccccgtgtct ctccaaatct tgggggagac
     1321 ggttcgcatg ctgactttcc gggtccggct gacgtgtttc tttgaaggga agcgtcctta
     1381 tgccatactg ttctgagaaa tttgaatctc agaggtgaaa gggagccggt ctcagcaggc
     1441 gattgtggat aattccgaaa catctcccat gatgcagcaa tacctggaga tgaaggagca
     1501 gtgcggggat gccatcctgc tgttccggtg cggggatttt tatgaaacct tcggggaaga
     1561 tgccaggatc gtctcgaagg ctctcaacat cgcgctgacc acacgtgacc gcaataccga
     1621 ccctaccccg cttgccggat ttccacatca cgcgctcgac cagtacctgc caaagcttgt
     1681 g
//
LOCUS       DAOQUC010001004         1680 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_96349_length_1680_cov_3.796923, whole genome shotgun sequence.
ACCESSION   DAOQUC010001004 DAOQUC010000000
VERSION     DAOQUC010001004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1680
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96349_length_1680_cov_3.796923"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(174..1487)
                     /locus_tag="PKH07_14985"
     CDS             complement(174..1487)
                     /locus_tag="PKH07_14985"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HNT36288.1"
                     /translation="MRNAIVLVPLLLCLFGCALLPRRLPQQEPIVRVLLFSDAREVTF
                     TSSEQFQCFRSQEQTSVLPANTQIQFRSLGQGIEWSMGQQSLGSAPEFVALAGRTPHA
                     ECMLSKVVGEQGDAEWPEGRKYQGILEIRRQSNGNLSGICQLPLEQYLCGVVPSEIGA
                     QAPFEAQKVQAIAARTETLSALADRRYAGDHYDICSTVMCQVFTGRTMCTPETDLAVF
                     ETRGVAMLHQGKTIGAFYASNCGGHSEHSENVWKARGFLPYCRGISDSSKRIRLDLSR
                     EDDFSKWIASMPDCYCNPSNPGIPDWAKRRFRWERRVSAEDLQRWVTKVKGIGRIQSV
                     KPLARGISGRIIELEFKGENGTCVAGPELPIRRLFEPMLYSAAFTIEPVGQEEFPASF
                     LIRGAGSGHGVGLCQVGAMGMAGRGMKCDKILKHYFTGIKLRKMY"
BASE COUNT          363 a          459 c          467 g          391 t
ORIGIN      
        1 gacaggctcg gcggccatct ggacgccttt actggacgcg aagtagtagg gttttacaac
       61 tccgtgttgg atgagcatct gccgcgcgca tttgatctga ttgctgatgt cgtcacggct
      121 ccggcatttg acccgacgga tgaatggttc gattgatcaa gatggcagga gcgtcagtac
      181 atttttctga gtttgatgcc ggtgaagtag tgcttcagaa tcttgtcaca cttcatgcct
      241 ctgccagcca tgcccattgc tccgacctgg cacaagccga cgccgtgtcc agagccagca
      301 ccacgtatca gaaacgatgc tgggaattcc tcttggccca caggctcgat ggtgaaggct
      361 gcgctgtaga gcatgggttc gaatagtcgt cgaatcggca gctcgggccc cgcaacacag
      421 gttccgttct cgcctttgaa ctcaagttcg atgatgcgtc ctgagattcc tctggcgagg
      481 ggtttgacgg actgaatgcg gccgatgccc ttgactttgg tcacccatcg ctgaagatct
      541 tcggctgaca cgcgccgttc ccatcgaaac cgtctctttg cccagtcggg aatcccaggg
      601 ttgctcgggt tgcagtagca gtccggcatg gaagcaatcc atttggaaaa gtcatcttct
      661 ctggagagat cgagacgaat ccgtttgctg ctgtctgaga tgcccctgca gtaggggaga
      721 aaaccccttg ctttccagac gttttcagag tgttcactgt gtccgccaca gttggaagca
      781 tagaatgcgc caatggtctt gccttggtgg agcatggcga cgccccgtgt ctcgaagacc
      841 gccaggtcag tctccggcgt gcacatcgtc ctgccggtga agacctgaca catgactgtg
      901 gagcagatgt catagtggtc acccgcatag cgtcggtctg ccagtgcgct caaagtttct
      961 gttcgtgccg cgatcgcctg aaccttctga gcttcaaagg gagcctgtgc accgatttcc
     1021 gacgggacaa ctccacagag gtattgttcg agtggcagct ggcagatacc tgataggttt
     1081 ccattgctct gtctccgtat ttccagaatg ccttggtatt ttctgccctc aggccattcg
     1141 gcgtcgccct gctcgccaac gaccttcgac agcatgcatt cggcatgtgg tgttctgccc
     1201 gcaagcgcta caaactcggg agcgctgcca aggctctgtt gacccatgct ccattcgatg
     1261 ccctgcccaa gagatcggaa ctggatctgt gtgttcgcgg gaaggacaga cgtctgttct
     1321 tgtgaccgaa aacactggaa ttgttcggat gacgtgaaag tcacctcccg cgcatccgag
     1381 aacaaaagca ctcgcacaat gggttcctgt tgtggcagcc ttctgggcag cagcgcacaa
     1441 ccgaacagac agagaagcag cggaacgagc acaatggcgt ttctcatgga caaaatctcc
     1501 tgtagcagag ccaaatccga actgtgaacc ggagtctatt atcaacaaag agaagcagat
     1561 tcgtcaacaa gactcaaccg ttgaatggta ctggctcaga tatgctggtg atacagccag
     1621 gatatgcttc ggaagcagag cggagtcttc ggagcaacgc taccgaagcc cgctgtctgg
//
LOCUS       DAOQUC010001005         1680 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_96351_length_1680_cov_3.672615, whole genome shotgun sequence.
ACCESSION   DAOQUC010001005 DAOQUC010000000
VERSION     DAOQUC010001005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1680
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96351_length_1680_cov_3.672615"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..456)
                     /locus_tag="PKH07_14990"
     CDS             complement(<1..456)
                     /locus_tag="PKH07_14990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36289.1"
                     /translation="MNSTLKAFFTQHTRLTLVLALAVLGGFLSLPLLGLLGCTSRAWA
                     QNARRQETSSEVTIAAVSYEGGDGTPTLEEINLFKNGIDDSLMNNIARYPWGESYIMM
                     SFVEMYKATRDLFFLDKMVQHADAVFANRDDVKGRVDMIRNKIMPAWGSG"
     gene            complement(838..>1680)
                     /locus_tag="PKH07_14995"
     CDS             complement(838..>1680)
                     /locus_tag="PKH07_14995"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNT36290.1"
                     /translation="QRHHNIVVGLYDLDYINRPFFATIITGVARQSAVFDFDLQFLSM
                     NPEIGVTEPDLYFLNRPRNRNVKGYIIIDQAVPDADILRLHEKGFPLVMIDREIEGAD
                     LPCVLSNEEKVLYDLTSYLLNKGRRNVAIVIASREWNREARKLRGFKAALADHGLQFD
                     EDLVFDTAYKEGIELTNLLLSILNRRDRPNAVITGRDDLAVQLIELAMDLGLNVPGDI
                     AITGYNDTEIASVYPVAITSVRVPLREMGERAIVLLENLIEEKPVEEHRLLLNPKIIV
                     RESC"
BASE COUNT          401 a          450 c          435 g          394 t
ORIGIN      
        1 accagaaccc caggccggca tgatcttatt tcgtatcatg tccactctgc ctttgacatc
       61 atctcggttg gcgaacacgg catcggcatg ttgaaccatc ttgtccagga agaacaaatc
      121 acgggtggct ttgtacattt cgacaaacga catcatgatg taactctcgc cccaggggta
      181 tctggcgatg ttgttcatga gtgaatcgtc aattccattc ttgaagagat tgatctcctc
      241 cagcgtggga gttccatccc ctccttcgta ggagaccgca gcgatggtga cttctgaaga
      301 tgtttcctgc cgacgtgcgt tctgtgccca ggctctcgaa gtgcatccca agagccccaa
      361 tagaggcaga gaaagaaagc ctcccaaaac tgcaagagcc aagacgagtg tcaaacgagt
      421 gtgctgtgtg aaaaaagcct tgagtgttga gttcaagctg gttccttcct tttgtgtgaa
      481 gagtgagagt acaactccgc tgcatcaaca cgacgggaat ctacttccac gcttcgaggg
      541 caacggacag attacgatac atcagactcc aaagcaggtg gcctcttcga gagaaaacgc
      601 ctccacagcg ctgaactcag cccacgcgca cacaggtgct aacctgcttt tggtaacacc
      661 gagtccagaa caagcgtgtc ctttgccgag caggtttcca cgggagtggg catagcccac
      721 acgttcacag gtgctaacct gcttttggta acaccgagtc cagaacaagc gtgtcctctg
      781 ccgagcaggt ttccacggga gtgggcatag cccacgcgct cgcaggtgct aacctgctta
      841 gcaggattcc ctgacaatga tcttgggatt gagcagcagg cgatgttcct caacaggctt
      901 ttcctcgatc aggttctcca gaagaactat cgcccgttct cccatttctc gaaggggcac
      961 tcgaaccgag gtgatggcga cagggtagac cgaagcgatc tccgtgtcat tgtagcctgt
     1021 gatggcgatg tcgccaggaa cattcaagcc caagtccatg gccagttcga ttaactgaac
     1081 tgccaggtcg tcgcgtcccg tgatcaccgc attggggcgg tctcgtctgt tgaggatgct
     1141 cagcaacaga ttggtcagtt cgattccttc tttgtacgct gtgtcaaaaa ccaggtcttc
     1201 atcaaactgc aatccgtggt ctgccagagc cgctttgaag cctcggagtt tgcgcgcttc
     1261 tcggttccat tcacgcgatg caatgacgat ggccacattc ctgcgtccct tgttgagcag
     1321 ataggaggtg aggtcgtaga gtaccttctc ctcattggac aaaacacagg gcaggtcagc
     1381 accttcgatc tcgcggtcga tcatcacaag agggaatccc ttctcatgaa gccgcagaat
     1441 gtctgcatcg ggaaccgcct gatcaataat aatgtaaccc ttgacgttac ggtttcgtgg
     1501 gcggttcaga aaatacaaat ctggttcggt gactccaatc tccgggttca tggaaagaaa
     1561 ctgcaggtca aagtcaaata ccgcactctg ccgggcaaca cctgtgatga tggtcgcgaa
     1621 aaagggtcgg ttaatgtaat ccaggtcgta cagccctacc acaatgttat ggtggcgctg
//
LOCUS       DAOQUC010001006         1678 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_96537_length_1678_cov_3.279729, whole genome shotgun sequence.
ACCESSION   DAOQUC010001006 DAOQUC010000000
VERSION     DAOQUC010001006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1678
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96537_length_1678_cov_3.279729"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..917)
                     /locus_tag="PKH07_15000"
     CDS             complement(<1..917)
                     /locus_tag="PKH07_15000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36291.1"
                     /translation="MVTWTDEARRHLETYLKSVSELTRAQGDDPDEIVASLRAHVQQE
                     IERQGLEYISPEWINRVISDLGSPEEIANVEAHPRQRQDDRFTVPGITPQSVPTEVVP
                     DLPGSRTCTFIWVFCISLGAFSLVFELLTGAMANIWRDPVPTPLHLIALVMILASLVL
                     SDRFLKPNSALRSRRLFGAVLILNGFAVFVAGLYTLAYLFVLPIAVLCLLGMGLGLMG
                     FAPLFCFLGTLSQMRWLYRNHSVLRVSGLWTMKRTLAGVACALCLGSFVEGPRIAAER
                     ALHMAISKDSNLKERGIRWIKVLGAEDQVL"
     gene            complement(960..1319)
                     /locus_tag="PKH07_15005"
     CDS             complement(960..1319)
                     /locus_tag="PKH07_15005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020148329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HNT36292.1"
                     /translation="MAHDEGHLDLRNWTTQIRKGLLELCALRLLADGEMYGYDLVKRL
                     ASAKTLVVTEGTVYPLLSRLHKAGLVKSRLEESSSGPARRYYSLTEEGKETLVLMQMY
                     WEEVQRSVASLKLQIQS"
BASE COUNT          458 a          450 c          403 g          367 t
ORIGIN      
        1 aggacttgat cctcagcacc aagcaccttg atccatcgga tgcctctctc cttaaggttc
       61 gagtctttgc tgatagccat atgcaaagca cgctctgccg cgatgcgagg cccctcgaca
      121 aatgaaccca aacacaaggc gcatgcgacg ccagcaagcg tcctcttcat cgtccacaaa
      181 ccggagaccc gcagaacgga atggttccga tagagccaac gcatttgcga aagagtcccc
      241 agaaagcaga aaagaggcgc aaaccccatt aaaccaagtc ccatgccaag aaggcaaagc
      301 accgcaatcg gtagcacaaa caggtatgcc agagtgtaaa gcccggcaac gaacacagca
      361 aagccattga gtatcagaac tgcgccaaac aaacgacgac tgcgcaatgc actgtttggc
      421 ttcagaaaac gatctgaaag gacgagactg gccagaatca tgacaagtgc gatcaagtgc
      481 aatggagtcg gtacgggatc tctccaaata ttagccatag caccggttag caactcaaaa
      541 accaacgaga atgcgccgag tgagatgcag aagacccaga tgaacgtaca ggtgcgagaa
      601 cccggaagat cgggtacgac ctcggttggc actgattgtg gcgtgattcc cggcactgtg
      661 aaacggtcgt cctgcctctg tcttgggtgc gcctccacat tcgcgatttc ctcgggactt
      721 cccagatcgg atatgaccct gttgatccat tcgggagaaa tgtattccag accttgcctt
      781 tcgatctcct gttgaacatg tgcgcgcaga ctggcaacaa tctcatcggg atcatccccc
      841 tgcgcccgag tcagctcaga tacagacttc aagtatgtct ccaaatgcct ccgtgcttca
      901 tctgtccacg taaccatcca cgtcctccat tcttggaatc gataggtgca ccccacgagt
      961 caactctgga tttgcagttt gagcgaggca acactcctct gaacttcctc ccagtacatt
     1021 tgcatcaaaa caagtgtctc cttaccttct tcggtaagtg agtagtaccg acgcgcaggg
     1081 ccgctcgagg attcctccaa acggctcttg acaagtccgg ccttgtgcaa tctcgacagc
     1141 aatgggtaga ctgtcccctc tgtcacaaca agcgtctttg ccgaagcgag gcgcttcaca
     1201 agatcatacc catacatctc tccatcagcc aagaggcgca atgcacacaa ctccagcaga
     1261 ccctttcgaa tctgggtagt ccagttcctt aaatctagat gtccctcgtc gtgtgccata
     1321 gtactacctt gcgtctgcca acctaggaag aatatataac aaggtaactt gtattgcaag
     1381 ctatcatgta acagtggctc tgaacgtgat gagatgatta gtaaattgga tttgcttgaa
     1441 atgaaaatgg attccgtcgt acattcagta gaaggagagg ttcacagtgg atgttccaaa
     1501 ttccgtgaaa gccatgagag gtggggcgta gttggctacg ccataattca agaaagggga
     1561 ttctcgatgg gcacagatcg caaagagatt ttctcggaga aggtttcagc cggacgtcga
     1621 acgtgtttca ttgatgtcaa ggagacggtc gaaggaaata gatatctcgt catctccg
//
LOCUS       DAOQUC010001007         1677 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_96633_length_1677_cov_3.545006, whole genome shotgun sequence.
ACCESSION   DAOQUC010001007 DAOQUC010000000
VERSION     DAOQUC010001007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1677
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96633_length_1677_cov_3.545006"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..919
                     /locus_tag="PKH07_15010"
     CDS             <1..919
                     /locus_tag="PKH07_15010"
                     /inference="COORDINATES: protein motif:HMM:NF024758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HNT36293.1"
                     /translation="KSSLRFFGPDRMGTGIVIDEHRHILTAESLLRNLDSVPEYTIWI
                     HADSVTSEISMKAEIVGSDPFTGIVILKPIPKTPLEEAVAQQLQPVELARGYEAKPGS
                     IVIMISNAYGMKHSCFWGILSGRQKHLDCWLMHDYLQSTLPLHPGAVGAPVCDRNGHV
                     VGIMAASFRKAPWQEVSFAIPTSRFLSAIPILIEQGCVPRGYLGVGISDSAEKRSQYG
                     IPEGISGVLVTQVLENSPAANAGIAPGDVIQEVLSTRIFSVPEVLWTIANSVPESVAE
                     IVVWRAGEFRNFNLVLGNSCEETAWNVPR"
     gene            931..1542
                     /locus_tag="PKH07_15015"
     CDS             931..1542
                     /locus_tag="PKH07_15015"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HNT36294.1"
                     /translation="MREIQTINACRLLSGNPVMILTTSLHERYNAMPLSWAMPCSQEP
                     PCIAISLTPSQHTYHVLRETGEFVLNIPNRRMLNRVHALGKATGRHRDKLSELMWPTR
                     FGRFVRAPYLTDCLAHIECQVQSHQTVGDRRLFVAQVMVACADAEAFDTHWTEQADTL
                     HYLGGNEYLCQGKILYATDQTEDLKREAGGGLDLFLRPIEDLN"
BASE COUNT          367 a          458 c          459 g          393 t
ORIGIN      
        1 gaaatccagt ctgcgttttt ttggccccga ccgtatggga actggaattg tcattgatga
       61 acatcggcac atccttaccg cggagtctct tcttcggaat ctggattcag ttccagaata
      121 cacgatttgg attcatgccg attcagtcac gtcggaaatc tcgatgaaag ccgagatcgt
      181 cgggagcgac cccttcacgg gtatcgtgat cctgaaaccc atccccaaaa cacctctgga
      241 agaggcggtt gcccaacaac tacagcccgt cgagttggca agaggttacg aagccaagcc
      301 gggcagtatc gtgatcatga tttccaacgc ctacggcatg aagcattcct gcttctgggg
      361 tatcctgagc ggacgacaga agcatttgga ctgttggctg atgcatgact atcttcagtc
      421 cactctgcct cttcaccctg gagccgtggg tgctccagtg tgtgatcgaa acggtcacgt
      481 tgtcggcatc atggcggcgt cttttcgaaa agcgccgtgg caggaagtgt ccttcgccat
      541 tccgaccagt cggtttctca gcgcgatccc gattctgatt gaacaaggct gcgtgccacg
      601 gggatatctg ggtgtgggga tctcggattc agcggagaag cgatcccagt atggaatccc
      661 cgaagggatt tccggcgttc ttgtaactca ggtgctcgag aacagccctg cggcaaatgc
      721 cggcatcgct cccggagatg tcattcagga agtgctctca actcgcatct tcagtgttcc
      781 ggaagtactt tggaccatag ccaactctgt gccggagtca gtggcggaga ttgttgtctg
      841 gcgcgcaggc gagtttcgca acttcaattt agttctcggt aactcgtgtg aggaaaccgc
      901 ctggaacgtt ccgcggtaag ggacagaacc gtgcgggaaa tccaaaccat caatgcctgt
      961 cgccttctga gcggcaatcc ggtcatgatc ctgacgacca gtctgcatga acgctacaac
     1021 gcgatgcccc tgtcctgggc aatgccttgc agtcaggagc ccccctgcat cgccatcagt
     1081 ctgacaccca gccaacacac ctatcatgtc cttcgggaga ccggagagtt tgtcctcaat
     1141 attcccaacc gacggatgct gaatcgcgta cacgcactgg gtaaagcaac cggtcgccat
     1201 cgtgacaaac tgtccgagtt gatgtggcct acgcgctttg ggcggttcgt tagagcgccc
     1261 tatctgaccg actgtctggc gcatatcgag tgccaggttc aatctcatca aacagtggga
     1321 gaccgacgcc tgtttgtcgc ccaagtgatg gtcgcctgtg cggatgcaga agcgtttgac
     1381 actcactgga cggagcaggc ggacacgctt cactatctcg gtggaaacga atacctctgt
     1441 cagggcaaga ttctctatgc aaccgatcaa acagaagacc tcaaacgaga agccggaggc
     1501 ggactcgatc tgtttctgcg acctatcgaa gatttgaact gatctgtctc ttcctccgtt
     1561 gacgggattt ccttgagtgc ctattctctt ggttgaatca gtgaggccga tccgttggat
     1621 cagcagagaa ggtttgagcg tggaagaccc agttcggctc caagcgtgtt tgtccgc
//
LOCUS       DAOQUC010001008         1677 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_96655_length_1677_cov_2.928483, whole genome shotgun sequence.
ACCESSION   DAOQUC010001008 DAOQUC010000000
VERSION     DAOQUC010001008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1677
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96655_length_1677_cov_2.928483"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..831
                     /locus_tag="PKH07_15020"
     CDS             <1..831
                     /locus_tag="PKH07_15020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36295.1"
                     /translation="AVILIWKQIPRQQGSTRTVSWVLRLFGIVLLTGLALMYRVTEGG
                     NTFGIRTMWWGILGLIGWAYLTTCFVYAITQGSPVGMMGAFGLLLCVFMGDRAGVFSS
                     WDLIKQKMDIRTMIGSHGAIATAGAFLGTLLLSGSKTQSHLARIRAAVLFGFGMAAAA
                     VLLRPIWGISKVKATPSWCLWGAAWTCWCWVALYWIIDVRGWKRWTWALAPAGENPLM
                     AYILAPMVYAFLQWTGVGFYERIAATSASLAILRGVIFAFAVTWSTAFLKRIGIRLAL
                     "
     gene            complement(948..>1677)
                     /locus_tag="PKH07_15025"
     CDS             complement(948..>1677)
                     /locus_tag="PKH07_15025"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNT36296.1"
                     /translation="LKMLSLIVSEHPERLKPFVMLDPRRSGSAELAIQCLERMDFCGV
                     KIYPSLGYSPDPYDVLNDKHSSANLEAIYGYCEGHSIPITAHCSRGGAYGEHILPEHH
                     IRDAYAHPRCWETVLKKHNRLILNLAHFGGDLGLVEDDSWSGAILEMMKDHPTLYADL
                     SCNPQALERKSSVQYFKSLRRVLDDPLTRERILFGTDWFMLRHTWTESEIVRVFGELP
                     KDDFEQITFRNPSAFLFPNSCRPR"
BASE COUNT          371 a          421 c          477 g          408 t
ORIGIN      
        1 gcggtgatct tgatctggaa gcagatccct cgacaacaag gcagcaccag aacggtttcg
       61 tgggtgttgc gactgttcgg catcgttctt ctgacaggac tggcacttat gtatcgcgta
      121 accgaaggag ggaatacttt tgggatccgt acaatgtggt gggggattct cggattgatt
      181 ggatgggcgt atctcacaac gtgtttcgtc tacgccatca ctcaggggag tcccgtgggg
      241 atgatgggtg cctttggact gctgctgtgt gtcttcatgg gtgaccgcgc aggcgtgttt
      301 tcctcatggg atctcatcaa gcagaaaatg gacattagaa cgatgatcgg gtcgcacgga
      361 gctattgcga cggccggggc atttcttggc acccttctct tgtccggttc taagacgcaa
      421 agtcacttgg cgcgcattcg agctgcggtg ttgttcggct ttggcatggc tgccgccgcc
      481 gtattgctcc gaccgatctg gggcataagc aaagtgaaag caaccccgag ctggtgcctt
      541 tggggtgcgg catggacgtg ttggtgctgg gtggcactgt actggatcat tgatgttcgc
      601 ggctggaagc ggtggacttg ggcactggct ccggcagggg aaaatccact gatggcgtat
      661 attctcgcac ctatggtcta cgcattcctg caatggactg gcgttgggtt ctacgaaagg
      721 atcgccgcga cgagcgcttc tcttgccatc ctcagaggtg tgatctttgc ctttgcggtg
      781 acgtggagca ctgcattctt gaagagaatc ggcattcggc tcgcactcta ggcacatagg
      841 ctgagttccc atcgtccaga caccatccgg cgaatgaatc aggaactcgt ttccctctca
      901 acgagtggaa ctgcacgagg cctgacgttc ctctgcgcgt aacacattca acgcgggcga
      961 cacgagttgg ggaaaagaaa cgcactcgga ttcctgaaag tgatctgctc aaagtcatct
     1021 ttcggcagtt caccaaacac tctgacaatt tccgattctg tccaggtgtg tctcagcatg
     1081 aaccaatctg taccaaaaag tatccgctct cgcgttaagg gatcatcgag aacccgtctc
     1141 aggctcttga agtactgcac gctgctcttg cgctcaagcg cctgaggatt gcaggacaga
     1201 tcggcataga gggttggatg atctttcatc atctcgagga tcgctcctga ccatgaatcg
     1261 tcctcgacca atccgagatc cccaccaaag tgcgccagat tgagaatcag ccgattgtgc
     1321 ttcttcaata cagtctccca acagcgagga tgggcatagg cgtcacggat gtgatgttct
     1381 ggaaggatgt gctctccata ggctccgccc cgtgaacagt gcgccgtaat cggaattgaa
     1441 tgaccttcac agtaaccgta gatggcttcg agatttgctg aggaatgttt gtcattgagc
     1501 acatcgtagg gatcagggct gtaaccgaga gagggataga ttttcacgcc gcaaaaatcc
     1561 attcgttcga gacattggat ggcaagttca gcgctgcccg atcgccgggg atccaacatc
     1621 acaaaaggct taagcctttc gggatgttcg gaaacaatca aagagagcat cttcaac
//
LOCUS       DAOQUC010001009         1676 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_96744_length_1676_cov_2.698951, whole genome shotgun sequence.
ACCESSION   DAOQUC010001009 DAOQUC010000000
VERSION     DAOQUC010001009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96744_length_1676_cov_2.698951"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            26..265
                     /locus_tag="PKH07_15030"
     CDS             26..265
                     /locus_tag="PKH07_15030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006422159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4258 domain-containing protein"
                     /protein_id="HNT36297.1"
                     /translation="MDETGHFCDMLKERRIEREWVDRTIREPERTEDHDDGTRHFIRQ
                     IPEFGDRWLRVVVNVKAHPERWVTAFFDRRLRRSE"
     gene            262..462
                     /locus_tag="PKH07_15035"
     CDS             262..462
                     /locus_tag="PKH07_15035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008870513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2283 domain-containing protein"
                     /protein_id="HNT36298.1"
                     /translation="MRVKLDRASDALYFRLDENRIVESEEIRPGVILDFDENDRVVGV
                     EFLGISQRATEEELSTMQFQTV"
     gene            complement(681..>1676)
                     /locus_tag="PKH07_15040"
     CDS             complement(681..>1676)
                     /locus_tag="PKH07_15040"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HNT36299.1"
                     /translation="RLSDSRSGWIRERGEDGAQVRWLSEGTIPPHNYFTSCEITGDDQ
                     YDRIVFSLREPVAFAVSSEINPTNRLIIDIFNTHFATTWMSHKAGAKFIGPVTGEQIE
                     DDCLRVTVPINSKQIWGYWTERSGNTLTVFVRRPPQIAVAPDSPLKGLKFALEAGHGG
                     SSAGAVGTMGTKEKSINLMAVTELERLLKERGAEVIQVRPGDSEPLFRDRLAWTNEFG
                     AHMYIAVHANAAGSSRGFLKISGTSTYYRDIHCWLPSQLVYDKLLGLGWGEFGVVGNF
                     SYYPLRDTRTPGILIEQAFMSNPYDEARMLEPEYQKAQAEVVVEALEEFFDKVRE"
BASE COUNT          387 a          468 c          455 g          366 t
ORIGIN      
        1 gtctgggctg tggtaggatt cattcatgga tgagactggc catttttgtg acatgctcaa
       61 ggagcgcaga atcgagcgcg agtgggttga ccggacgatc cgagagcccg agcgaacaga
      121 ggaccatgac gacggcacac gacacttcat ccggcagata cccgagttcg gggatcgttg
      181 gctgcgcgta gttgtgaacg tgaaggcaca tccagaacga tgggtgacag cattcttcga
      241 ccggagattg aggagatcag aatgagagtc aagttagata gagccagcga cgcactctac
      301 ttccggctcg atgagaaccg gatagtcgaa tccgaggaaa tccgtccagg tgttatcctc
      361 gacttcgacg agaatgacag agtggtgggt gttgaattcc tcggcatctc gcagcgtgca
      421 accgaagagg aactctcaac catgcaattc cagacagtat gaagccgttt tggttattcc
      481 tacgctctcc caaacaggcc agcacctgcg cgcgtgtggg ctataccctc ttcccctttg
      541 gacgtttgct caatacatga cctgcatcta ttggactcac tgtcacgaga agaacagcag
      601 ccccgcggcg ttgttccagc ggtgactttc gcattaccgc atcaatccca gacaatcccg
      661 tgggtcacga cgcacgctac ctactctctg actttatcga agaactcctc cagtgcctcg
      721 acgacgacct ccgcttgtgc tttctggtac tcaggctcca gcattcgtgc ctcatcatag
      781 ggatttgaca taaaggcttg ctcgatgagg atgccgggag ttcgagtgtc tctcagagga
      841 tagtaggaga agttcccaac cacaccaaac tcaccccagc ccagtccgag cagcttgtcg
      901 tacaccagtt gcgatggcaa ccagcagtga atgtcacgat agtaagtgct tgtgccggag
      961 attttgagaa agcctctgga gctgccagcg gcgttggcat gaaccgcaat atacatgtga
     1021 gcaccaaact catttgtcca ggcaagtcga tcgcggaaca gaggctccga atctcccggg
     1081 cgaacctgaa tcacttccgc accgcgttcc ttgagcagcc gttccaactc cgtcactgcc
     1141 atcaggttga tgctcttctc tttcgtcccc atggtgccaa ccgcaccggc agaggaacca
     1201 ccatgccccg cttcgagcgc aaatttcagc cctttcaacg gggaatcggg tgcgacggca
     1261 atctgtgggg gacggcgaac aaacacggtc agggtattgc cactgcgctc agtccagtag
     1321 ccccaaatct gcttgctgtt gatgggaacc gtcacgcgaa gacagtcatc ttcgatctgc
     1381 tctcccgtca cgggtccgat gaacttggca ccggctttgt ggctcatcca ggttgtcgca
     1441 aagtgcgtat tgaagatgtc aatgatgagc ctgttggtgg gattgatctc ggaagagacg
     1501 gcaaacgcca ccggctccct gagagagaat acaattcggt cgtactgatc gtcacctgtg
     1561 atctcgcagg aagtgaagta gttgtgaggc ggaatggttc cttcggaaag ccatcgcacc
     1621 tgtgctccgt cctcaccccg ttcgcgaatc catccggaac gagaatccga caggcg
//
LOCUS       DAOQUC010001010         1675 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_96837_length_1675_cov_2.962963, whole genome shotgun sequence.
ACCESSION   DAOQUC010001010 DAOQUC010000000
VERSION     DAOQUC010001010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1675)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1675)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1675
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96837_length_1675_cov_2.962963"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="PKH07_15045"
     CDS             <1..274
                     /locus_tag="PKH07_15045"
                     /inference="COORDINATES: protein motif:HMM:NF013236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor C-terminal
                     domain-containing protein"
                     /protein_id="HNT36300.1"
                     /translation="LSEDRKNALYSRVYGTVVPVIAVLGTEQVTVGDVLSLKIGDTVH
                     LNRRLRDDIDIFVGTEHRFKAKPGVYGANKGIKITRVLEEDREVAS"
     gene            271..1311
                     /gene="fliN"
                     /locus_tag="PKH07_15050"
     CDS             271..1311
                     /gene="fliN"
                     /locus_tag="PKH07_15050"
                     /inference="COORDINATES: protein motif:HMM:TIGR02480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HNT36301.1"
                     /translation="MSLSQEEIDALLKGQGDIEKKKDGSALDDNAQVVLDSFGTLWNE
                     SIADALAGHTEEPLGARVTDVGSLSVKDIRESLTDEDVVFFVGVNPPLKTPMLFLFPA
                     ASCARLGCLLSGQEMQDELTDEHRGVLANFWDQIAGIWAAKLSDKYGLKLRPDSTQVM
                     LGSEMLAGMDVLYEDIVAPIGFVECLLDFPTWLEGMLRCLFGKDVIDALEPIDASAPS
                     PTKKAAAPKPQPVQSGRKTEAPTQTLQPVVFEPLVQDAQPSEPSNLDLILDIKMEIRV
                     ELGRTNYKVREILELCPGYVVELNKLAGEPVDLLVNDKLFARGEVVVIDENFGVRITE
                     ILSLKERIESLR"
     gene            1396..>1675
                     /locus_tag="PKH07_15055"
     CDS             1396..>1675
                     /locus_tag="PKH07_15055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36302.1"
                     /translation="MRSSSFFTWVVPIFSDSCQTQRWSCFCVSSFRAVQLLQALRLCP
                     LLQTPAHWKKQVSSKSGFLRGLALMVGLCLLASAVSLGAVVARADEAEP"
BASE COUNT          390 a          395 c          484 g          406 t
ORIGIN      
        1 actctcggaa gacagaaaga acgctctcta cagtcgagtt tacggaacag tagtgccagt
       61 gatcgcagtc ttgggaaccg aacaagtgac cgtgggcgat gtactcagtc tgaagatagg
      121 ggataccgtc catctgaaca ggcgtctacg cgatgatatt gatatctttg tcggaacgga
      181 gcatcggttc aaagcaaaac caggggtcta tggggcaaac aagggaatca aaatcacccg
      241 ggtgttggag gaggacaggg aggtggcttc atgagcctgt cgcaggaaga gatcgatgcc
      301 ctcttgaagg gacagggaga catcgagaag aaaaaagacg gaagtgcttt ggacgacaat
      361 gcccaggtgg tcttggattc cttcggcact ctctggaacg aatccattgc tgatgctctt
      421 gccggtcata cggaagagcc actgggtgcc cgtgttacgg atgtgggaag tctctccgtc
      481 aaagatatcc gggagtccct gaccgacgaa gatgttgtgt tctttgtggg agttaatccc
      541 cctttgaaaa ccccaatgct ttttctgttc ccagccgcca gttgtgcccg gctgggttgt
      601 cttctttcgg gtcaggagat gcaggatgaa ctgaccgatg aacaccgtgg agttctggcg
      661 aatttctggg atcagattgc gggaatctgg gcagccaagc tctccgacaa gtacggtttg
      721 aaactcagac cagactcgac ccaggttatg ctcggttccg agatgctggc tggtatggac
      781 gttttgtatg aagacatcgt ggctccgata ggttttgtcg aatgcctgtt ggacttcccc
      841 acatggttgg aaggaatgct gcgttgtctc tttgggaagg atgtcataga tgccttggag
      901 ccaatagacg cttccgcacc cagtcctaca aagaaggcag cagcgccaaa accccaacct
      961 gtccaatcag gtcggaaaac agaggcacca acgcaaacac tacaacccgt ggtgtttgag
     1021 ccattggttc aggatgcgca gccgtcagaa ccctcgaatc tggacttgat cctggacatc
     1081 aagatggaga tccgagtgga actgggtcga accaactaca aggttcgcga gattctcgaa
     1141 ctgtgtcctg gctatgtggt tgaactgaac aagctcgctg gcgaaccagt cgatctgctg
     1201 gtgaacgaca aactctttgc gcgtggggaa gtggttgtta ttgacgagaa tttcggcgtg
     1261 cgcatcaccg agattctaag ccttaaagaa cgcatcgaat cgctgagata gtgatctcgg
     1321 caacggcagc aagtgaatct ttgagggcgt gtcttggttt ggtgccgtat tccgaagcgg
     1381 gagaagagat tccggatgcg aagcagtagt ttctttacgt gggtggtgcc cattttttct
     1441 gactcctgtc aaacacagag atggagttgc ttttgtgttt cctcatttcg agcagtccag
     1501 ctcctgcagg cgctcaggct atgcccactc ctgcaaacgc ccgctcattg gaagaaacag
     1561 gtttcctcca agtctggttt tctaagaggc ctcgccttga tggtggggct gtgtctcctt
     1621 gcttctgcag tctctcttgg tgcggttgtc gctcgtgcgg atgaagcaga gccta
//
LOCUS       DAOQUC010001011         1675 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_96844_length_1675_cov_2.763580, whole genome shotgun sequence.
ACCESSION   DAOQUC010001011 DAOQUC010000000
VERSION     DAOQUC010001011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1675)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1675)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1675
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96844_length_1675_cov_2.763580"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..688
                     /locus_tag="PKH07_15060"
     CDS             <1..688
                     /locus_tag="PKH07_15060"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HNT36303.1"
                     /translation="AMVQRILLGISRRLSRFAQRLNPDPHQESLRLWFEQKGDKTHRV
                     QYDLREDSVVFDLGGYEGAWASDIFSRYVCTIYVFEPVAQFVAEIRRRFARNPKIGVY
                     DFGLSDRDQEASLSISSSSSSLFKSGGKVERIRLRQASAFMKEKGISHVDLMKINIEG
                     GEYDLLEHLLDVGFIEHITNLQIQFHDFVPQSRQRMEVIQRRLSETHELTYRFPFIWE
                     NWQRKGLRHK"
     gene            685..1563
                     /locus_tag="PKH07_15065"
     CDS             685..1563
                     /locus_tag="PKH07_15065"
                     /inference="COORDINATES: protein motif:HMM:NF013681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-1,2-fucosyltransferase"
                     /protein_id="HNT36304.1"
                     /translation="MIIVRLNGGLGNQMFQYALGRNLAGILKTQLKLDRSAFRDDPLR
                     EYALSPFAIQEDFATDKEVADLVFTRRTLLRRVLDKALQRRPQYASSYIREKSKFHFD
                     PEILNLPDGIYLDGSWQCEKYFASNAPTIRQEFTVKEPLKGDNADMADRISACDAVSL
                     HVRRGDYVSDPKTNRIHGVCDLEYYSRAIEYLVERVSAPRLFVFSDEPDWATEHLHFD
                     IPSQVVGHNPPDQGHEDLRLMALCKHHVIANSSFSWWGAWLCVHISPIVICPSRWFRS
                     QEMDSGELIPERWTKI"
BASE COUNT          411 a          392 c          460 g          412 t
ORIGIN      
        1 ggcgatggtg cagagaatcc ttctcggaat aagccgccgg ttgtcgcgtt tcgctcaacg
       61 actgaatccc gatccgcacc aggaatctct tcgcctatgg tttgagcaaa aaggcgacaa
      121 gacgcataga gtccagtacg acctcagaga ggattctgtg gttttcgatc tgggaggcta
      181 tgaaggggcg tgggccagcg acatcttctc acgctatgtt tgtacgatct atgtgtttga
      241 gcctgttgcg cagtttgttg cagagattcg gcgccgtttc gcacggaatc caaagatcgg
      301 agtctatgat tttgggcttt ccgaccgaga ccaagaagcg agcctttcca tcagttcttc
      361 atcgtcctct ctattcaaat caggcgggaa agtcgagcgc attcgacttc ggcaagcaag
      421 cgcgtttatg aaggagaagg gcatatctca cgtagacctg atgaagatca acattgaggg
      481 aggcgaatac gatctgctcg agcatctgct tgacgttggt ttcattgaac acatcacaaa
      541 tctgcagatc cagtttcatg atttcgtgcc gcagagcaga caacgcatgg aggtcatcca
      601 aagaaggctc tctgagaccc atgaattgac gtatcgtttt cccttcatct gggagaattg
      661 gcaaaggaag ggcttgcgcc ataaatgatt atcgtgagac tcaacggtgg gttgggaaat
      721 cagatgttcc agtatgcgct ggggaggaac cttgccggaa tactgaagac gcaactcaaa
      781 ctcgaccgca gtgcatttcg agatgaccct ttgagagagt atgctctttc gccctttgcc
      841 attcaggagg attttgccac ggataaagaa gttgcagatc tggtgttcac aaggagaacg
      901 ctcttgaggc gggtactcga taaggcgctc cagcgcaggc cccagtatgc gtcaagttac
      961 atccgggaga agagcaagtt tcatttcgat cctgagattc tgaatcttcc tgatggcatc
     1021 tacttggacg gatcatggca gtgtgagaag tactttgcct caaacgctcc aacaattcgt
     1081 caggagttca cagtgaaaga accgttgaag ggcgacaatg cggacatggc ggatcgtatc
     1141 agcgcctgcg atgcagtgag ccttcatgtc cgccgtggag attacgtttc cgatcccaag
     1201 acaaatcgaa ttcatggagt ctgcgacctt gagtattact ccagagcaat tgagtacctc
     1261 gtcgagagag tttctgcccc tcggctgttt gtctttagtg atgaaccgga ttgggcgaca
     1321 gagcatctcc acttcgatat cccatctcaa gtggtcggac ataatccacc cgatcaggga
     1381 catgaagatt taaggctgat ggcactgtgc aaacatcatg tgatcgccaa cagctcattc
     1441 agttggtggg gagcatggct gtgtgttcat atctcaccca tcgtcatctg ccccagtcgg
     1501 tggtttcgat cgcaggaaat ggactcgggg gagttgatcc ctgagagatg gacgaagata
     1561 tgaaactgtc cgttgccgca aggagatgag gaagaagcgt tgagaattct gtttgtaacg
     1621 tctgctgcgc ctgcgagaag cgctttcagc acttcggaga agagacctcc gttgg
//
LOCUS       DAOQUC010001012         1674 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_96946_length_1674_cov_2.571958, whole genome shotgun sequence.
ACCESSION   DAOQUC010001012 DAOQUC010000000
VERSION     DAOQUC010001012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1674)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1674)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1674
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96946_length_1674_cov_2.571958"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1674)
                     /locus_tag="PKH07_15070"
     CDS             complement(<1..>1674)
                     /locus_tag="PKH07_15070"
                     /inference="COORDINATES: protein motif:HMM:NF014348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide lyase family 8 super-sandwich
                     domain-containing protein"
                     /protein_id="HNT36305.1"
                     /translation="SDPLYGTFGDVTDERSHMLTHSGLWARSVRTLALAYSTPASRYH
                     HSDEILQSIAFALKGYGRWVYPGCPKPANWWAWDIGMPMNLVDAYILLQDSLPVTQAD
                     FLRDTLKHLVRTGVQKGLGANAIWVAWIQFRFGMSVDNLDYVQWGYETLNRQSQISAQ
                     SGDGIKPDYSYHFHAPGMNMGYGVSHYADISRYLFLTQGTPYAMQEPSVHVRWYLEYI
                     RWLVFRDADDLYAYGRAGTRTDGKERAAIILESTLPFVALDSPDHDEIVADCKRLLAD
                     VPEFFSLLHASEQLTVEKSRVAPAVLEGFRYYPYSSCGIWRGRDFMGTVRLDSPRNKK
                     WFSIHNENLLGHRIADGHLPLWVDGFETAGGVLPCQDWERLSGITGAAGFVLPRESLG
                     QSTLSGGVSLENKYGLAAMELIVKPAQDGATLSARKSYLFLPDAAICLVSDACVSPST
                     AAAVPYTVLAQIPWNLPDKRPGLTLDGKELPLIEDKEWTQAFQWLHFRERGLVNLMPR
                     DIHLTWQTTTSPWSRINQIMAPDREMDRLYTCSFLVAEHVHSCTDALAY"
BASE COUNT          399 a          474 c          471 g          330 t
ORIGIN      
        1 cataggccaa cgcatctgtg cagctgtgga cgtgttcggc aacgagaaag gagcaggtgt
       61 agagcctgtc catctcgcgg tcaggagcca tgatctggtt gatacgcgac cagggagaag
      121 tggttgtctg ccatgtcaga tgaatgtctc tcggcatcag gttcacaagt ccgcgctcgc
      181 ggaagtgcag ccattgaaat gcctgtgtcc attccttgtc ctcaatcagc ggaagttcct
      241 tgccatccag ggtcagtcca ggacgtttgt cgggaagatt ccagggaatc tgcgccagaa
      301 ccgtgtacgg tacggcggcg gcggtggacg gcgaaacaca cgcatcactg acaagacaga
      361 tcgccgcatc gggaagaaac agataactct tccttgctga caatgtcgcc ccgtcctgag
      421 caggcttgac gatcagttcc atcgctgcca aaccgtattt gttctcaagc gacacccccc
      481 cggaaagagt gctctgtcca agggactcgc gaggcagcac aaaaccggca gcccccgtga
      541 tgccactcag ccgctcccag tcctgacaag ggagaacccc acctgctgtt tcaaagccat
      601 ccacccacag gggaagatgc ccgtcagcaa ttcgatgtcc cagcagattc tcgttgtgaa
      661 tcgagaacca tttcttgttc ctcggcgaat cgagtcggac ggtgcccatg aagtcacggc
      721 cgcgccatat cccgcaagag gaatagggat agtaccgaaa tccttcgagc accgcgggcg
      781 ccacgcggct cttttcgaca gtcaactgct cagaggcatg caacagcgag aagaactccg
      841 gcacatccgc caggagacgt ttgcagtcag cgacaatctc gtcatggtca gggctgtcca
      901 gcgcaacgaa cggaagcgtg gactcaagga tgatcgcagc gcgttccttg ccatccgttc
      961 gagtgcccgc tcgcccatac gcatagagat cgtccgcatc gcggaagacg agccaccgaa
     1021 tgtactcaag ataccaccgc acatgaaccg aaggttcttg catcgcatag ggcgttccct
     1081 gagtcagaaa caggtatcgg gagatgtctg catagtgcga aacgccgtaa cccatgttca
     1141 tgccgggagc gtgaaagtga taggagtagt cgggcttgat tccgtcgccg gactgagcgc
     1201 tgatctgact ctgacggttg agcgtctcat atccccattg gacgtaatcg agattgtcaa
     1261 cgctcatgcc aaatcggaat tgaatccagg caacccaaat ggcgtttgcc cccaagcctt
     1321 tctggacgcc ggttcgcacc agatgcttca gcgtgtctcg gagaaaatca gcctgagtga
     1381 caggaagact gtcctgcaag aggatgtatg catccacaag attcatgggc attccaatgt
     1441 cccaagccca ccagttggca ggtttgggac accctggata gacccatcgt ccatatccct
     1501 tcagtgcaaa ggcaatgctc tgaagaatct cgtcgctgtg atgatatcgg ctggcaggcg
     1561 tcgagtaagc cagggcaaga gtccgaacgg agcgtgccca gaggccggag tgagtgagca
     1621 tgtgggaacg ctcgtcggta acatctccaa aggtgccgta gagcggatcg gact
//
LOCUS       DAOQUC010001013         1672 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_97163_length_1672_cov_2.401979, whole genome shotgun sequence.
ACCESSION   DAOQUC010001013 DAOQUC010000000
VERSION     DAOQUC010001013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97163_length_1672_cov_2.401979"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1115)
                     /gene="aroC"
                     /locus_tag="PKH07_15075"
     CDS             complement(<1..1115)
                     /gene="aroC"
                     /locus_tag="PKH07_15075"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HNT36306.1"
                     /translation="MLRFLTAGESHGRGLVAIVEGMPSGMSLIAEDINPDLKARQGGY
                     GRGGRMKIESDQVQILSGVRWGQTLGSPIALLIENKDFANWETKMSVHPEHRDPTIAI
                     QEPRPGHADLVGLLKYNRPDIRDILERASARETAARVAVGAVCRKLLQQFSIESVSHV
                     VSIGTVVAPVQSLTFEEIRARQAASDLKCADPKAEMTMKVAIDLAKHEGDSLGGVVEV
                     RVKGLPVGLGSHVHWDRKLDGKLAQSLMSIQAIKGVEIGLGFQSAFLDGSKVHDEIST
                     DGSVFLRLSNNAGGLEGGMTTGEDLVLRAAMKPIATLQRPKKTINPFTKQPASASTER
                     SDVCAVTAAAVVAEAATLFTVAQAFCEKFGGDSLKEM"
     gene            1356..>1672
                     /locus_tag="PKH07_15080"
     CDS             1356..>1672
                     /locus_tag="PKH07_15080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012255482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36307.1"
                     /translation="MTESLFGPQQIVEMLHEIASQIQRRNPGRELAFVGIRARGVPLA
                     HRLQTILEQEYSRRTFFGILDITLYRDDSVLFGGIAVGGIRISHLSDIEKDLTMQMTV
                     SRTS"
BASE COUNT          388 a          470 c          426 g          388 t
ORIGIN      
        1 ttcatttcct tcagactgtc accgccgaac ttctcgcaaa acgcctgcgc tacggtgaaa
       61 agtgttgctg cttccgccac gaccgcagcg gcggtgactg cacaaacgtc cgaacgctct
      121 gtcgaggctg acgcgggttg cttggtgaaa ggattgatgg ttttcttggg gcgctgcagt
      181 gtcgcaatgg gtttcatggc tgctcgcagc acgagatcct caccggtggt cattccgcct
      241 tccagaccgc ctgcgttgtt gctcagacgt aagaaaacgc tcccatctgt tgatatctca
      301 tcatgcacct tcgaaccgtc caggaaggca gattgaaagc ccaagccaat ctccactcct
      361 ttgatggctt gaatggacat cagcgactgc gccagtttcc cgtcaagttt ccgatcccaa
      421 tggacatggc ttcccaaacc gaccggcaaa cccttgacac ggacttcgac aacaccaccg
      481 agagaatctc cttcatgctt ggctaaatca atggcaacct tcatcgtcat ctcggctttt
      541 ggatcggcac acttgagatc cgaagcggct tgacgtgccc taatctcctc aaacgtcaga
      601 ctttggaccg gtgcgacaac agtgccgatt gagacaacat ggctgaccga ttctatggaa
      661 aactgctgaa ggagtttcct gcaaacagcg ccaacagcca cacgtgcggc ggtttctctg
      721 gcactggcac gctcaagaat gtctcggatg tcgggacggt tgtacttcag cagacccacc
      781 agatcggcat gccccggacg aggttcttgg atggcaatgg tcggatcccg atgttcggga
      841 tgtacggaca tctttgtttc ccagttggca aaatccttgt tttcgatcag cagggcaata
      901 ggggaaccaa gggtctgccc ccatcgaacg ccggagagaa tctgaacctg atcagattcg
      961 attttcatcc ggcctccgcg cccatatcct ccctgacgtg ccttgagatc agggttgata
     1021 tcctctgcaa tgaggctcat tcccgaaggc atgccttcca caatggcaac cagcccacgg
     1081 ccatgggatt cacccgctgt cagaaaacgc aacatcagtc tcacaacctg cttgttatcg
     1141 agaattcatc tgcaaataac ctaccacacc cgcccacgat ctgcaacgtt gacatgtccg
     1201 cattctcatt ccccacttga caaccgaggc aaagatcctt attcaagcat gagggatagt
     1261 ttgatgtggt tggtggctga aacgaaggat aattctgtgg tgccgaaaca accgatcgag
     1321 gtgctgtccc aagcaattgg aaggtggcta ggaccatgac ggaaagcctc tttggaccgc
     1381 aacagattgt cgagatgctc catgagattg ccagtcagat tcagcgccgc aatcccggca
     1441 gggagctggc ttttgttggc atcagggcgc ggggtgtccc gttggcgcat cggcttcaga
     1501 cgattctgga gcaggagtat tcgcgcagga cattctttgg aatcctcgat atcacactct
     1561 accgcgatga tagcgtacta tttggcggta ttgcggtagg cggcatcaga atttcccatc
     1621 tatccgacat tgaaaaggat ttgaccatgc agatgaccgt aagccgaacg tc
//
LOCUS       DAOQUC010001014         1671 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_97217_length_1671_cov_3.229579, whole genome shotgun sequence.
ACCESSION   DAOQUC010001014 DAOQUC010000000
VERSION     DAOQUC010001014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1671
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97217_length_1671_cov_3.229579"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..236
                     /locus_tag="PKH07_15085"
     CDS             <1..236
                     /locus_tag="PKH07_15085"
                     /inference="COORDINATES: protein motif:HMM:NF024584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HNT36308.1"
                     /translation="TVPLCIGDTNEKYLIGDATKQSLYDIWHSDAMKEVRRLHNARKR
                     LDLTPCMECPEGRLETVTENGVIPKDMGNLRNV"
     gene            462..>1671
                     /locus_tag="PKH07_15090"
     CDS             462..>1671
                     /locus_tag="PKH07_15090"
                     /inference="COORDINATES: protein motif:HMM:NF012876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HNT36309.1"
                     /translation="MRKPSWFAGWRWFGIRGKLLIGIVLLLSISTATQVLIVLSLVQA
                     SDRWSAIDLQLHERRDLARQIRIAFSEELSSLRAYFAFGDFRMREPVLYWKTQVETLL
                     EEAQRAPFAQELDPSSTASSFQLLNQMSEAHAKLGQLFIQEMDLRLQGLDDQWRKMAL
                     ENNVELVNRLFTCLDLYVQQNDEMLRSERERRVQDKAAVLGQIFALVGVSLGMALVFS
                     LMVTRKVSWPLKQLSQATRDLTLGNFRLLSGTFSEDEVGELARSFNEMSVALEERQAY
                     IIQRNRELTTLNRLTALLATAANLNGCAEDIVTMITDALPANRTGFYIFEPKVGSFVL
                     RAHRGLPIPFVEGCGASLDQPELLGAKVPLDQSVIMDSLTESPNPQVQLLRNEGFSSA
                     IIVPVATRKSL"
BASE COUNT          395 a          432 c          461 g          383 t
ORIGIN      
        1 gaacggtgcc cttgtgcatc ggggacacaa atgagaagta cctgatcggc gacgcgacca
       61 agcaaagcct gtatgatatc tggcattctg acgcgatgaa ggaagttcgc aggttgcaca
      121 atgcgcgcaa gcgtttggat ttgacaccgt gtatggaatg tcctgagggg cgtctggaaa
      181 cggtcacaga aaacggtgtc atcccgaaag acatgggaaa tctcagaaat gtgtgacctc
      241 tgtgccggtt cttgatctgg cgggtttcaa acagcaggag cattcttcgg atccgacaga
      301 tcctcgaatc gaacagacca gcgcctgcag gcgtgtgggc tatgcccact cttacgaacg
      361 cttgctcaat ggaggaaaca gacttcttca aagtctgatt tcccaggagt ctcgacgagc
      421 gtcctttgtg aagcgggacg tcctcaaagg agcaccacac gatgagaaaa cccagctggt
      481 tcgctggctg gcgttggttt ggaatccgag ggaaacttct catcggaatc gttttgcttc
      541 tctccatcag cactgcaact caggtcttga ttgttctcag cctcgttcag gcgagcgacc
      601 gctggagtgc cattgatctc cagcttcacg aaagacgcga tctcgcccgt cagattcgga
      661 tcgcgtttag cgaggagttg tcctcactca gagcatactt tgcgttcggt gattttcgga
      721 tgcgagagcc cgtgctgtat tggaaaaccc aagtggaaac acttttggag gaggcgcaac
      781 gggctccttt tgcccaggag ttggatccga gttctacggc atcctcattc caactgctga
      841 accagatgtc tgaagcacac gccaaactcg ggcaattgtt cattcaggag atggatttgc
      901 ggctccaggg acttgacgat cagtggcgta agatggcact tgagaataac gtcgaactgg
      961 tcaaccggtt gttcacctgc ctcgaccttt acgttcagca aaatgatgaa atgctccgaa
     1021 gcgaacgaga aagacgtgtt caggacaagg cagctgtact tggacagatt tttgctttgg
     1081 tgggagtctc cttgggcatg gcactggtct tcagtctgat ggtcacccgc aaagtctcat
     1141 ggccgttgaa gcaactctcc caggccactc gagatctcac cctgggcaat ttccgtctgc
     1201 tcagcggaac cttctcagaa gacgaagtcg gagaactggc acgctccttc aacgagatgt
     1261 ccgttgcctt ggaagagaga caggcataca tcatccagcg aaacagggag ctgaccaccc
     1321 tcaaccgact gacggcgctg ctcgcaacgg cggccaatct gaatggctgc gctgaagaca
     1381 tagtgacgat gataacagat gccctgcccg ccaatcgaac gggtttctac attttcgaac
     1441 cgaaggtcgg aagttttgta ttgcgagcgc accgaggtct gcccatcccc tttgtggaag
     1501 gctgtggagc gtctttggat cagccggaac tgctgggagc aaaggtgcct ctggatcaaa
     1561 gtgtcatcat ggattcgctg acggagtctc caaatccgca agtgcagttg ctgagaaacg
     1621 aaggcttttc atctgccata atcgtgcccg ttgcaacccg caaatcgctg c
//
LOCUS       DAOQUC010001015         1670 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_97339_length_1670_cov_2.735604, whole genome shotgun sequence.
ACCESSION   DAOQUC010001015 DAOQUC010000000
VERSION     DAOQUC010001015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1670
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97339_length_1670_cov_2.735604"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="PKH07_15095"
     CDS             <1..196
                     /locus_tag="PKH07_15095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HNT36310.1"
                     /translation="KRFETDMAEILTLLSRRPCTVDDLADGLQIHRNEALKCVQQLLQ
                     GNLIEEIRRDNATYYLPRKP"
     gene            complement(251..1117)
                     /locus_tag="PKH07_15100"
     CDS             complement(251..1117)
                     /locus_tag="PKH07_15100"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNT36311.1"
                     /translation="MLRRLIGEDIELVWRSNPRLWPVMMDPSQLDQILANLSVNSRDA
                     IEGVGTLTIETDNVTFNEKDAGRDTICEPGEYVMLSVSDTGIGMSQEVQAHLFEPFFS
                     TKEVGKGTGLGLATVYGIVKQNSGFIEVQSEQKKGTAVRIYLPRALAEETSEAPVAEN
                     KSPRGTETVLIVEDEEAVLILVKVILERHGYTVFATRKPEDALALAETCKDSIHLLIT
                     DVIMPSMNGRVLEQRIRRVLPDIKVLFISGYTANVIAQHGVLKEGIHFLQKPFSNSDL
                     TRKVRRVLDQKS"
     gene            complement(1172..>1670)
                     /locus_tag="PKH07_15105"
     CDS             complement(1172..>1670)
                     /locus_tag="PKH07_15105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNT36312.1"
                     /translation="YQREELLNKSARILYENDEDYEYVGREKYRQISERGTGTVETCF
                     RRKDGSIIHVILSSTPIDPSDLSRGVTFTALDITERKRMEEERIVLQERLIQAQKMES
                     VGRLAGGVAHDFNNMLQAILGYCHLATDMVASDSVLYANLQEIRKAAERSANLTRQLL
                     AFARR"
BASE COUNT          397 a          470 c          399 g          404 t
ORIGIN      
        1 caaacgcttc gagacagata tggcggagat tctcaccctg ctgtcaagga gaccctgtac
       61 ggtggacgac ctcgcagacg gtctccaaat ccatcgcaac gaagcgctca agtgcgttca
      121 gcaattactc caagggaatc tcatcgagga aatcagaaga gacaacgcca cttactatct
      181 gccacgcaag ccttagaacg tggcagagcc ttcgtgttca acgtccatga aagatacgtg
      241 gccaccacta tcaagatttc tggtcgagga ctctgcgaac cttgcgcgtc agatcagagt
      301 ttgagaaggg tttctgcagg aaatggattc cctccttcag cactccgtgc tgggcaatga
      361 catttgctgt gtaacctgag ataaaaagaa ccttgatatc tggtaacaca cgcctgatcc
      421 gctgttccag aacccgtcca ttcatggaag gcatgattac gtcagtaatg agtagatgga
      481 tcgagtcttt gcatgtttct gccagagcca atgcatcctc cggcttgcgg gtagcaaaga
      541 cagtgtagcc atgccgttcg agaatcacct tcaccaatat gagaaccgct tcctcatctt
      601 ccacgatcag aactgtttcc gttccacgag gagacttgtt ctcagccacg ggtgcttcac
      661 tggtctcctc tgccagagcg cgagggagat aaatcctgac tgctgttccc ttcttctgct
      721 cgctctgaac ctcgatgaat cctgagttct gtttgacgat tccgtagaca gtcgccagtc
      781 ccaagccggt tcccttcccc acttcctttg tcgagaagaa aggttcaaag agatgagcct
      841 gaacttcctg actcatcccg attcctgtgt cgctgactga gagcatgaca tattcacccg
      901 gttcacagat ggtatctctt cccgcatctt tctcattgaa ggtgacgttg tcggtctcaa
      961 tggtcagtgt gccaactccc tctatcgcat cccttgagtt gacagagaga ttggccagaa
     1021 tctgatccag ctgagaggga tccatcatga caggccaaag tcgagggttg gaacgccaca
     1081 ccaactcgat atcttcacca atcaggcgcc gaagcatctg cagcatgcct ccaaccgccg
     1141 cgttgagatc aatgacgcgc gggctgatcg tctaacgccg tgcaaaagcc agaagttgtc
     1201 gggtcagatt cgcggaacgc tcggcagcct tccgaatctc ctgcagattg gcatacagca
     1261 cactgtcaga cgccaccata tcagtcgcca gatgacagta tcctagaatc gcctgaagca
     1321 tattgttgaa atcatgcgca acccctccgg caagacgacc tactgattcc atcttctggg
     1381 cctggatcag acgctcctgg agtacgatcc gttcttcctc catgcgcttt ctttcggtga
     1441 tgtccaaagc cgtgaaggta acccctcgag agagatctga aggatcaatg ggcgtgctgc
     1501 tcagaatgac atggatgata ctaccgtcct tgcgtcggaa acaagtctcc acggttcccg
     1561 tgccacgctc cgatatctgt cggtatttct cgcggccgac atactcgtag tcttcgtcat
     1621 tctcataaag aatgcgggca gacttattga gcagttcttc ccgttggtac
//
LOCUS       DAOQUC010001016         1668 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_97511_length_1668_cov_2.666460, whole genome shotgun sequence.
ACCESSION   DAOQUC010001016 DAOQUC010000000
VERSION     DAOQUC010001016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1668
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97511_length_1668_cov_2.666460"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="PKH07_15110"
     CDS             <1..208
                     /locus_tag="PKH07_15110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36313.1"
                     /translation="TFVLTNPNFEYLGRSVCHGLYAFPAAREARLSMQSLKLESLRLR
                     VPCFLVFDSDSLPTAARNAAAQVL"
     gene            553..>1668
                     /locus_tag="PKH07_15115"
     CDS             553..>1668
                     /locus_tag="PKH07_15115"
                     /inference="COORDINATES: protein motif:HMM:NF024205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36314.1"
                     /translation="MVPGRFSKPVEIEVTGSMRFMNIKTSLFSLLLAMLMLSAVEAYS
                     QTDKVQALTEEALRQHIAQIEKATDLDDALRNQVLEFYREALQQAQRASEWRSKAASY
                     QTEGKNASSLIETMRKSLEGTLKEPQVPDKPLAELEQLRIQTEAALAATQSDLAQRDE
                     EFRNRVNRRMEIPNLLRQAQTDLQEINRQLAAIVPAIDENPHLEQARQAQLTAHKQAV
                     EEQINALNQELVHYEARVSLLPLRRDIAERDVRGTRALLEALQKKIGELRRREAADAV
                     AETRRDLENAHPVARKIALDNTTLANLRTETAAKVEQTNKTITQVNQRLSQIRDEYKG
                     IQEKVARIGMSNAIGLLLQRKQGELPDIRKRSREARSS"
BASE COUNT          430 a          450 c          464 g          324 t
ORIGIN      
        1 tacctttgta ctcacgaatc cgaacttcga atatctcggt cggtctgttt gtcacggtct
       61 gtatgccttt cctgctgccc gcgaagcgag actttcgatg cagtcgctca aactcgagag
      121 tctccgcctc cgcgtgcctt gttttttggt cttcgattca gacagcctcc cgaccgctgc
      181 caggaacgca gcggctcaag tcctctgatc tcgacagact caatctaaca cggacacgca
      241 ggaagaccaa atcgaaaaga acagcaaaca cattggctca ggtgcggagg ggtggaggta
      301 tgcttcggtt gcagaggctc tgcgacgctg ccgaagtggg aacagccaga aggcgggctt
      361 cggtagcgtt gctccgaaga ctccgctttg cttccgaagc atatcttggc tgaaacacca
      421 gcataactga gccagtacca gcaaacacat tctttgacat attcctttgt ctctcggcaa
      481 caaaaacagt attgtaatac cggctatatg cccaccggtt tttggacggt atgggcagga
      541 acgtgggaga gtatggtgcc cggccggttc agcaaacctg tggagatcga ggtgactggc
      601 tcaatgcgat tcatgaatat caagacatct cttttttcgt tgttgctggc gatgctgatg
      661 ctgtctgccg tcgaagccta ttcgcagacc gacaaagttc aggctttgac ggaggaagca
      721 ctcagacaac acatcgccca gattgagaag gcgaccgatc ttgatgacgc actccgaaat
      781 caggtcttgg agttctaccg agaggcgctt cagcaggcgc agcgcgcctc ggaatggcga
      841 tccaaggctg cgagctatca gacggaagga aagaacgctt cttccctgat agagacgatg
      901 aggaagagcc ttgaaggaac tctgaaagaa ccacaggttc ccgacaagcc cctggcggaa
      961 ttggagcagc tgagaattca gacggaagct gcgctggccg ccacgcagtc cgacctggcg
     1021 cagagggatg aggagtttcg caacagagtc aatcgcagga tggagattcc caacctgctt
     1081 agacaggcac agaccgacct gcaggagatc aacaggcagt tggccgccat cgtacccgcg
     1141 attgatgaga atccacatct cgaacaagcg cgccaagctc agctgacggc tcacaaacag
     1201 gcggtggaag aacagatcaa tgccctaaat caggaactgg ttcattacga agctcgcgtc
     1261 agtctgttgc cccttcgacg ggatattgcg gaacgtgatg tccgggggac gcgggcactg
     1321 ctcgaagcgc ttcagaagaa gatcggtgaa ctcagaaggc gggaggcagc cgacgcggtc
     1381 gcagaaacgc ggcgcgatct ggaaaatgcc catcctgtgg cgcgcaagat cgcccttgac
     1441 aacaccactc ttgcaaacct cagaactgaa accgccgcga aggttgagca aaccaataag
     1501 accatcacac aagtcaacca acgcttgagc cagattcgcg acgagtacaa gggtattcag
     1561 gagaaggtcg cccgaattgg aatgagcaat gccatcggtc tgcttctgca gagaaagcaa
     1621 ggcgaactgc ctgacatccg aaaacgctcc agggaagccc gatcatcg
//
LOCUS       DAOQUC010001017         1667 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_97605_length_1667_cov_2.902605, whole genome shotgun sequence.
ACCESSION   DAOQUC010001017 DAOQUC010000000
VERSION     DAOQUC010001017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1667
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97605_length_1667_cov_2.902605"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..100)
                     /locus_tag="PKH07_15120"
     CDS             complement(<1..100)
                     /locus_tag="PKH07_15120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HNT36315.1"
                     /translation="MRTNVISFITDFGTRDNFVGVMKGVILTINPEA"
     gene            300..>1667
                     /locus_tag="PKH07_15125"
     CDS             300..>1667
                     /locus_tag="PKH07_15125"
                     /inference="COORDINATES: protein motif:HMM:NF012927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HNT36316.1"
                     /translation="MSQFESFRYRCLDEVLETLNQLGIAWPRSDDFSVLFEPVEIHGK
                     RLANRFVIQPMEGCDCNPDGSPTALVRRRYRRFAAGGAGMLWCEACAVVPEGRANPRQ
                     MMLTDANVGKMASMLDDCRAAAQESMGSAHQPLFILQLTHSGRYSKPEGKPAPIIAHH
                     SSVLDPLMKIPADYPLISDEQLERLQDAYLKAASLAYEAGFDGVDVKACHGYLLAELL
                     ASFTRTGSRYGGEEFESRTRMVREIHQRIHASFPSLIVTSRMNVYDPIPYPYGWGCDR
                     GDYRTWDLREPIKLVGALEEQGAECVNITIGNPYYLPHYNRPYDTPVAGFQPPEEHPF
                     VGVHRMLEIVREIQQAKPEVRVVGSGYSYLRQFMPWFASGLVRAGWVSLVGMGRGALA
                     YPDFVKDLAETGSMNPFKTCITCSNCTQIMRDGGTTGCVIRDDEVYGAVYREGRRWVE
                     DTIRRA"
BASE COUNT          372 a          408 c          474 g          413 t
ORIGIN      
        1 ctgcttcggg attgatggtc agaatgaccc ctttcatcac acccacgaaa ttgtctcggg
       61 tgccaaagtc cgtgatgaat gaaatcacat tggttctcat gggatatcct tccttcagcg
      121 aactgcacat attgaccaaa aatatcactg aaaagagata ttcgtcaacg gagagcacct
      181 tctgttgaag acgctgttga ccttgcgccc atcattgttt agtttactct tcataatcag
      241 atgcgcactt tggtgtcttg tggcttcagg ctgcaatatg tccggtgctg ggaggaattg
      301 tgagccagtt tgagagcttt cgttaccgat gtctggacga ggttcttgag acactcaatc
      361 aactggggat tgcgtggccg agatcggatg acttttctgt cttattcgag cccgtagaga
      421 ttcacggcaa gaggcttgcc aaccggtttg tcatccaacc gatggagggt tgtgactgca
      481 atccagacgg ttccccaacg gcactggttc gtcgccgata ccgccgtttt gccgctggcg
      541 gtgcggggat gctctggtgt gaagcatgtg ctgttgttcc cgagggaaga gccaacccaa
      601 ggcagatgat gctgacggat gccaacgttg gaaagatggc atccatgctt gatgactgtc
      661 gtgctgcagc ccaagagtca atgggatctg cccatcagcc attgttcatc ctgcaactca
      721 ctcactctgg tcgttacagc aagccggagg gaaagccagc accaatcatt gcccatcatt
      781 cgtctgtgct ggatcctctc atgaagattc ctgcagacta tcccttgatc tcagacgaac
      841 aactggagcg tcttcaagat gcttacctta aagccgcttc gttggcctat gaggcgggct
      901 ttgacggagt ggatgtcaaa gcctgtcacg gttatctgct cgcggaactc ctcgcgtcct
      961 ttaccagaac ggggagccgc tatggtggcg aggagttcga gagccgaacc cgaatggttc
     1021 gagagatcca tcagagaatc catgccagtt tccccagcct gatcgtgaca tcacggatga
     1081 atgtatacga tcccatcccc tatccgtatg ggtggggctg cgatcgcggg gactatagga
     1141 catgggacct gcgtgaaccg atcaaacttg ttggtgcgtt agaggaacag ggagcagagt
     1201 gtgtcaacat cacgataggc aacccgtact acctgccaca ttacaacaga ccttatgaca
     1261 cccctgttgc aggatttcag ccacccgaag agcatccatt tgtgggagtg cataggatgc
     1321 tcgagattgt gcgtgagatc cagcaggcga agcctgaagt gcgggtcgtt ggatcaggat
     1381 acagctatct tcgtcagttc atgccttggt ttgcctcagg tcttgtgagg gctggatggg
     1441 tttcacttgt cggaatgggg cgcggtgctc ttgcgtatcc cgattttgtg aaggacttgg
     1501 ctgaaacggg gagcatgaat ccgttcaaga catgtattac gtgtagtaac tgcacccaga
     1561 tcatgcggga cggtgggaca acgggttgtg tcatccgaga tgatgaggtc tacggagccg
     1621 tctaccgtga gggtcgccga tgggtggaag acacgattcg gcgtgcg
//
LOCUS       DAOQUC010001018         1667 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_97625_length_1667_cov_2.359181, whole genome shotgun sequence.
ACCESSION   DAOQUC010001018 DAOQUC010000000
VERSION     DAOQUC010001018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1667
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97625_length_1667_cov_2.359181"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1667)
                     /locus_tag="PKH07_15130"
     CDS             complement(<1..>1667)
                     /locus_tag="PKH07_15130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36317.1"
                     /translation="ACSTIAATRVRFAQVGSRYMEIEAKVTLGVENLEEALWLVKVEV
                     FDEDRALLRAATNEFSSSVLIVGDPVISTQEITVNLPTSHEETQKSAFFRVTLERLLS
                     RDREPGRTINDIPFLRPLSRSKPPQPVKADSQGNAVVSPLLFWSDSMLDVKQGQTISI
                     SAKGDVKGCHGPEGEWAYGPWGPTGGMSEGTEHYPKRLCALIGRIEGSDRCEEFLVGD
                     SISFEAPMDGRLYLGVSDIHHFDNEGEFIATVSIDGKHVNFRNLTTSLEVRVKEKIKA
                     RPKALYYQLWTAISHAEDLPSDARLWRDSKTGKVWRPCKSAQSLERKMIYDDLSPGEY
                     RASVHAVWEDRSQDPTPVGVSDPVELKQGDNRIATINLEGASILTVKAIDGSTQKSID
                     FVCVNVSGSDGFHVVGLYTGTDGMVSFTHLNPGEYTLQMERMLRQFGEPHYRPESEPV
                     TVTLEDGQATEITVPMKRIETSDSEAEKMYPWCVIGIVTDQDANPLADVEISASCGRG
                     TLFQTGKTVSGSDGKYTLRFGPGVRNPDEKTGEVTAGLQASTIYAFT"
BASE COUNT          334 a          495 c          448 g          390 t
ORIGIN      
        1 ccgtgaaggc gtagattgtt gaagcctgca ggccagccgt gacctcgccc gtcttctcgt
       61 ccggattgcg gacgccggga ccaaatcgca gtgtgtactt tccgtcggat ccgcttacgg
      121 ttttcccagt ctggaataaa gttccacgtc cacagctggc gctgatctca acgtccgcaa
      181 ggggattggc gtcttgatcc gtcacaattc cgatcacaca ccagggatac atcttctcgg
      241 cttcggagtc gcttgtctca atgcgcttca tcggaacggt gatctccgtt gcctggccat
      301 cttcgagagt aacggtgacg ggttcgctct caggcctata gtggggctca ccgaattgtc
      361 tcagcattcg ttccatctga agcgtgtatt caccagggtt gagatgcgtg aaagaaacca
      421 tgccatccgt tccggtatac agcccgacaa catggaaacc gtccgaccct gaaacattca
      481 cgcaaacgaa gtcgatcgac ttctgtgtcg agccgtcaat ggctttcact gtcaagatcg
      541 acgcgccttc cagattgatc gtcgcgattc tgttgtcgcc ctgcttcagt tcgacaggat
      601 cgctgactcc aacaggcgtc ggatcctgtg aacggtcttc ccaaacggca tgcacactcg
      661 ctcgatactc gcccggtgag aggtcgtcgt agatcatctt ccgttccagg ctctgagcgg
      721 atttgcaggg tcgccagacc ttgcccgtct tggaatctct ccagagccga gcatctgatg
      781 gcaggtcttc ggcatgcgag atcgccgtcc agagttgata gtagagagct ttgggccttg
      841 ctttgatctt ctccttcaca cgaacttcga gagaagtcgt cagattcctg aagttgacgt
      901 gcttgccatc aatgctgacg gtcgcaatga attccccctc gttgtcaaag tggtgaatat
      961 ccgaaacgcc cagatagagc cgaccgtcca tcggcgcttc gaaactgatg ctgtcgccga
     1021 caagaaactc ctcgcatcta tcgctgcctt cgatcctccc aatgagcgca cagagccttt
     1081 tgggatagtg ctccgtgccc tcagacatcc cgcctgtcgg tccccaaggc ccataggccc
     1141 attcgccttc cggtccgtgg caacccttga cgtcgccttt cgcggagatg ctgatcgtct
     1201 gaccttgctt gacgtcgagc atcgagtccg accagaagag caaaggggaa acgacggcgt
     1261 tgccctggct gtcagccttc acgggctggg gcggcttact gcgactcaac gggcgtagaa
     1321 agggtatatc gttgatcgtt cttcccggct ccctgtcgcg cgaaagcagg cgctccagag
     1381 tcacgcggaa gaaggctgac ttctgggtct cctcatggga ggtcggtaga ttcacggtga
     1441 tctcttgcgt tgagatgaca gggtcgccaa cgatcaacac ggaagaggag aactcgtttg
     1501 tcgcagcccg caagagcgct cggtcttcgt caaagacttc taccttgacc agccagagcg
     1561 cctcttcaag gttctcgact ccgagtgtta ccttcgcctc gatctccatg tatcgcgatc
     1621 caacctgagc aaagcgaact cgcgttgccg cgatcgtgct gcaggcg
//
LOCUS       DAOQUC010001019         1666 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_97680_length_1666_cov_3.232154, whole genome shotgun sequence.
ACCESSION   DAOQUC010001019 DAOQUC010000000
VERSION     DAOQUC010001019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1666
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97680_length_1666_cov_3.232154"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="PKH07_15135"
     CDS             complement(<1..405)
                     /locus_tag="PKH07_15135"
                     /inference="COORDINATES: protein motif:HMM:NF019924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HNT36318.1"
                     /translation="MKIAVVASEVVPFAKTGGLADVSGSLPPALEELGQDVCVFMPKY
                     RTVVEQGLQTEPTGVRLEIPIGGRTLEGSVEWAFLPNSSVKVYLVANDEFFDRPQLYG
                     ESGLDYVDNLERFAFFCRGVLQFLETANMATDI"
     gene            492..>1666
                     /locus_tag="PKH07_15140"
     CDS             492..>1666
                     /locus_tag="PKH07_15140"
                     /inference="COORDINATES: protein motif:HMM:NF015451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNT36319.1"
                     /translation="MKQAADAIVIGGGASGMMAAIVAARNGTSVRILERMQRVGKKLL
                     ATGNGRCNLSNQRVSIEDYHGTSPKFAAQVLHQFDCVQTMKFFEELGIAWRIEDHGRV
                     FPLSDQASSVLDVLRFELDRLGVPVECETIIQKVVRDDDGLSCLSSEGLAFKAHKVIV
                     ATGGKSAPNLGSNGGGFRIAQALGHHIVEPHPALVPVTLDCPALKHINGVKVIADAQA
                     LVDGVPQGREVGEVLFTEYGVSGSPILALSRIISESLLKGSESSIQLDLFPEMSFDEL
                     LSFLRNRFSVASEKSVSFSFVGLVHKRLISLILKEAEIQNAECSCSKIGESDISRIAK
                     ALKGLKFRCSGTLSWMYSQVTAGGVDVREVDRRTLESKVVPGVYFAGEVLDVDGNSGG
                     "
BASE COUNT          379 a          405 c          455 g          427 t
ORIGIN      
        1 tatgtcagtg gccatattgg ccgtctccaa aaactgaaga actccacggc agaaaaatgc
       61 aaaacgttcc aggttatcca catagtcgag tccagactcg ccgtagagtt ggggtcggtc
      121 gaagaactcg tcgttggcaa ctagatagac tttcacagaa ctgttgggaa gaaacgccca
      181 ctcaaccgaa ccttccaatg tccgtccacc aatcgggatc tccagacgca ccccagtggg
      241 ctcggtttgg agcccctgct caaccactgt tcggtacttg ggcatgaata cacaaacatc
      301 ctgtcccaat tcctccaatg caggaggaag agaacctgag acatcagcca agccgcccgt
      361 tttcgcgaaa ggaacaactt cggaagcaac tactgctatc ttcaatgact tgaccctccc
      421 tgttctggtt gtcaacttct acttttcact atagcatctg tctgcttaac gatcaagaag
      481 gagcgcaagt catgaagcaa gccgcggatg ctatcgtaat cggtggggga gcttccggga
      541 tgatggctgc aattgtagct gccaggaacg ggacttcagt caggatattg gagcggatgc
      601 agcgggtagg caagaaacta ctggccactg gaaacggacg atgcaatctc agcaatcaaa
      661 gggtttcgat tgaggactat catgggacat ctcccaagtt cgccgcacag gtgcttcatc
      721 agttcgactg cgttcaaaca atgaagttct ttgaggaact tgggattgcc tggcgcattg
      781 aggatcatgg gcgtgttttt cctctttctg accaggcgtc tagcgtattg gatgtactcc
      841 ggtttgagtt ggatcgtctg ggtgtgccag ttgaatgtga gacgattatc cagaaggttg
      901 ttcgggatga tgatggtttg tcctgcctat cgtccgaagg acttgccttc aaagcgcaca
      961 aggtgatcgt cgcaactggc ggtaagtccg cacccaatct gggttccaat ggaggaggat
     1021 tcaggattgc ccaagccttg gggcaccaca tcgtcgagcc gcatcctgcc ttggtgcctg
     1081 tgaccttgga ctgtcccgcc ttgaaacata tcaatggagt gaaggtcatt gccgatgctc
     1141 aagcgttggt tgacggagtt ccacagggaa gggaagtcgg cgaagtcctc ttcactgaat
     1201 acggtgtctc cggttcccca attcttgctt tgagtcggat catttccgag tcgctgctca
     1261 aggggagcga gtccagcatt cagctggatt tgtttcccga gatgtctttc gatgagctgc
     1321 tctcgtttct cagaaaccgg ttttctgtgg cttccgagaa gtccgtctcc ttcagttttg
     1381 tcggactcgt tcacaagcgg cttatctctt tgatcctcaa ggaggcggag atacagaatg
     1441 cagagtgttc atgctccaaa atcggtgaga gtgatatctc tcgcatcgca aaggctctta
     1501 aaggattgaa gtttcgttgt tctggtacgc tctcctggat gtactctcaa gtgactgccg
     1561 gtggtgttga tgtgcgggaa gttgaccgac gcactctcga gtccaaagtg gttccaggtg
     1621 tctatttcgc aggagaggtg ttggatgtgg atggcaactc tggtgg
//
LOCUS       DAOQUC010001020         1665 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_97766_length_1665_cov_3.319255, whole genome shotgun sequence.
ACCESSION   DAOQUC010001020 DAOQUC010000000
VERSION     DAOQUC010001020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1665
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97766_length_1665_cov_3.319255"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..278
                     /locus_tag="PKH07_15145"
     CDS             <1..278
                     /locus_tag="PKH07_15145"
                     /inference="COORDINATES: protein motif:HMM:NF024584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HNT36320.1"
                     /translation="PFNYFSDSGYLNRSRCYFLWKSASFNWDGSVSPCCCVYDPSTDF
                     GSICEEDFATIWNNDLFQSARAACTGRLAPKKAIRTVCSQCRVFRKA"
     gene            296..1471
                     /locus_tag="PKH07_15150"
     CDS             296..1471
                     /locus_tag="PKH07_15150"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HNT36321.1"
                     /translation="MRIAINAVFRWKPSGVANYICNLVRYLSRIDTENEYLIFTTPQN
                     ESYFPTDVPNFRQIHCASQTDSPIYRRLWEQVVLPGVLKKSHMDVLHCPMNVLPLRVS
                     CSTVLTIIDTQYFQNPDHFTFLRRTYLKHSMLRSFKKADALITISESVKQEILGCFPV
                     LDKKIQVVHFGLDPCFRRVEDERAIVQMRKRYGIQHKYLLFPGYPHYRKNLPRLVRAF
                     GAIRSRLLEPHILVIAGEMGTEETNVAEINRAIEEQSLKEHVLFTGYVPGICLQDNES
                     SMALLMSGASLLVYPSLYEGFGLPVLEAMACGTPVLASDLPISHEVAGDAAIFVNPYQ
                     IEDIARGVEQGVNDEALRKQLVAGGYERAGLFTWENNARMTLQCYREVVERKGRSRV"
     gene            1464..>1665
                     /locus_tag="PKH07_15155"
     CDS             1464..>1665
                     /locus_tag="PKH07_15155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36322.1"
                     /translation="MFSLRLIRLLKRQFGSFLRHNTCAKFRNLCCILYQQWQGTTDVK
                     GYPFEIVVDPTNICTLRCPLCVT"
BASE COUNT          394 a          430 c          438 g          403 t
ORIGIN      
        1 ctccgttcaa ctacttctcc gactcaggtt atctcaacag gagtcggtgc tacttcttgt
       61 ggaaatcagc gtcctttaac tgggatggga gcgtgtcgcc ctgctgctgc gtctacgatc
      121 cctcaaccga cttcggcagc atctgcgagg aggattttgc aaccatctgg aacaatgacc
      181 tctttcagag tgcgcgtgca gcctgcacag gccgtttggc tccaaagaag gcgatcagga
      241 cagtctgctc tcagtgcagg gtttttagaa aggcataaac aaggacaaac gacagatgag
      301 aatagcgatc aatgcagtgt ttcggtggaa gccgagtgga gtagccaact acatctgcaa
      361 tctagttcgg tatctgagcc ggattgacac agagaatgag tatctcattt tcacaacacc
      421 tcagaacgag tcgtactttc cgaccgatgt cccaaacttc agacagattc actgtgcctc
      481 gcagacggac agccccatct accgaagact gtgggaacag gtcgtcttgc ccggggttct
      541 gaagaaatca cacatggatg tgctgcactg tccgatgaat gttctgccct tacgggtctc
      601 atgctcgaca gtgctgacaa tcattgacac acagtatttc cagaatcccg atcacttcac
      661 tttcctgcgc agaacgtatc tgaagcattc catgcttcgc tcattcaaga aggcagacgc
      721 tctgattacc atctctgaga gcgtcaaaca ggagatcctc ggatgcttcc ctgtgctgga
      781 caagaagatt caggtcgttc atttcggact cgacccttgc ttccgaagag ttgaggacga
      841 gcgcgccatt gtgcagatgc gcaagaggta tggtatccag cacaagtacc tgctcttccc
      901 cggctatccg cattatcgaa aaaaccttcc gagactggta cgtgcctttg gcgcaattcg
      961 gagcagactc ttggagcctc acatccttgt catcgctggg gaaatgggaa cagaagagac
     1021 caacgtggca gagatcaacc gcgccattga ggaacaatcc ctcaaagagc atgtgctgtt
     1081 caccggatat gtgccgggaa tctgcttgca ggacaatgag tcgagcatgg cacttcttat
     1141 gagtggcgcg tcactgctcg tatacccttc tctctatgag ggcttcgggc ttccggttct
     1201 ggaggcaatg gcttgtggca ctccagttct cgcttcggat ctgccgatct ctcacgaggt
     1261 ggcaggggac gccgctatct tcgtcaaccc gtatcagatc gaggatatcg cccgtggagt
     1321 tgagcaggga gtcaacgatg aagccttaag gaagcaactt gttgctggag gctatgagag
     1381 agcaggcttg ttcacctggg agaacaatgc acggatgaca cttcaatgtt accgagaggt
     1441 agtcgagaga aaggggcgaa gtcgtgttta gcctgcggtt gattcgtctg ctcaagaggc
     1501 agttcggttc ttttcttcgc cacaatacgt gcgcgaagtt tcgaaacctc tgttgcattc
     1561 tctatcagca gtggcaaggc acgacagatg tcaagggata cccgtttgag atcgtcgtcg
     1621 atccgacaaa catctgcaca cttcgttgcc ctctgtgtgt tactg
//
LOCUS       DAOQUC010001021         1665 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_97767_length_1665_cov_3.300621, whole genome shotgun sequence.
ACCESSION   DAOQUC010001021 DAOQUC010000000
VERSION     DAOQUC010001021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1665
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97767_length_1665_cov_3.300621"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="PKH07_15160"
     CDS             complement(<1..330)
                     /locus_tag="PKH07_15160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36323.1"
                     /translation="MRKTSSLTTRTLTVLSTLLLLFGFSITQVSADEGLLAQFESDLQ
                     QRFALLSRSVVDIVVDVSGEDVFGHEGLRPSLGIPQPGLGGKPEPPTISDVTAMGGVD
                     CLQKSSLR"
     gene            complement(352..1092)
                     /locus_tag="PKH07_15165"
     CDS             complement(352..1092)
                     /locus_tag="PKH07_15165"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HNT36324.1"
                     /translation="MNCEQARESSTALCSNELSPEEVRNLIQHTDECASCRTNLKAMQ
                     SVWIMLEHWKVASPPKHCHADFHSRLTQAQRESSSTLGFLYRFGFPQQRLSLVGTALV
                     ATLGLALIVAAPNSFRTARRAMRSGRNGIAATDSIDTHSASEVIGGSLLGQQGGTMSS
                     VASSSNPTGSSRISMASMSQRMADTQDDSNLLPQSLRHASPLSSSAQRSIGVLVAADD
                     MSSRSVASQADFVQTEVFRVVPVGYSSH"
     gene            complement(1110..>1665)
                     /locus_tag="PKH07_15170"
     CDS             complement(1110..>1665)
                     /locus_tag="PKH07_15170"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNT36325.1"
                     /translation="ACQQGDSKALDKLYLRYYQKIFSFILRYVNDPDKAQDILQETFL
                     RVYNWREKYAPTAKFASWLYRIARNLCIDEKRKYWNRMVDLDSQSRLEEDAQGPIDLE
                     SDKGFDPRQSLDESIKMDLIRKAIESLSEEQRDVILLNKYEGLSYQEIGDILGISTES
                     VKQRAYRAHLRLREILEPLLAERD"
BASE COUNT          386 a          428 c          461 g          390 t
ORIGIN      
        1 acgcagactg gatttctgaa gacagtccac accgcccatc gccgtgacat cggaaatcgt
       61 aggaggctcg ggtttgccgc caagtcctgg ttggggaatg cccaaggaag ggcgcaggcc
      121 ttcatggcca aagacatcct caccagaaac gtccaccacg atgtccacta ccgagcggct
      181 gagcagagca aaacgctgct gaagatcgga ttcgaactgc gccagcaaac cttcatcagc
      241 gctgacttgg gtgatggaga acccaaagag cagaagaaga gtagacaaaa ccgtgagtgt
      301 gcgtgtcgtc aatgaagaag tctttcgcat acgttctgcc gcagtctcct ctcaatggga
      361 agaatacccc acgggtacaa ctcgaaagac ttcggtctgc acaaaatccg cctgagaggc
      421 gaccgagcga ctgctcatgt catcggcggc gacaagcaca ccaatggagc gctgggctga
      481 tgacgagaga ggactcgcat ggcgcagact ctgaggcaag agattgctgt catcctgagt
      541 gtcagccatt ctctgactca tcgaggccat tgaaatacgg ctgctgccgg tcggattgga
      601 agaggatgct actgaactca tcgtgccacc ctgttgaccc aagagtgagc cgccgatcac
      661 ctcagatgct gagtgggtgt caatggagtc tgtggccgca attccgttac gaccactccg
      721 catggcacgg cgagccgttc gaaaactgtt gggagcggcg acgataagag caagtcccaa
      781 cgtggcaacc aaggccgttc cgaccaaaga aaggcgttgt tgtgggaagc cgaatcggta
      841 caggaatccc agcgtggaag atgattccct ctgtgcctgg gtgaggcggc tgtgaaagtc
      901 tgcgtggcag tgcttgggtg gtgacgccac tttccaatgc tcgagcataa tccaaacgct
      961 ttgcatggct ttgaggtttg ttctacagga tgcgcactcg tcggtgtgtt ggatgaggtt
     1021 tcttacctct tcggggctca actcgttgga gcaaagagca gtggaagact ctcttgcctg
     1081 ttcacagttc atcgttctga cctcccaaac taatctctct ctgcaagaag cggctccagt
     1141 atttcgcgga gtctgagatg agcacgatag gctctttgct tgaccgactc cgtgctgata
     1201 ccaagtatgt cgccaatctc ctggtacgac agtccttcgt atttgttcag aaggatcacg
     1261 tcgcgttgtt cttccgaaag actctcgatg gcctttcgga tcaaatccat ctttatggac
     1321 tcatccaggc tctgcctggg gtcgaatcct ttgtccgatt cgagatcaat ggggccttgg
     1381 gcatcctctt caagacggct ttgggaatcc aagtcaacca ttcggttcca gtatttgcgc
     1441 ttttcatcaa tgcacaggtt tctggcgatg cgatagagcc aagaggcaaa tttcgcggtt
     1501 ggtgcgtact tctctctcca gttgtacact cggaggaagg tttcttgcag aatgtcttgg
     1561 gccttgtccg gatcattaac gtaccgcagg atgaaggaaa agattttctg atagtacctt
     1621 agatagagtt tatctagagc ctttgagtcg ccctgctgac aggcg
//
LOCUS       DAOQUC010001022         1663 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_97997_length_1663_cov_2.919776, whole genome shotgun sequence.
ACCESSION   DAOQUC010001022 DAOQUC010000000
VERSION     DAOQUC010001022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1663)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1663)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1663
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97997_length_1663_cov_2.919776"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(174..590)
                     /locus_tag="PKH07_15175"
     CDS             complement(174..590)
                     /locus_tag="PKH07_15175"
                     /inference="COORDINATES: protein motif:HMM:NF017020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HNT36326.1"
                     /translation="MTQENISALVNFRIEQAESSVRAATLLENEGAMREVIHLSYYAM
                     FYAVLALLAVEQRESSKHQGVISLFDRDYVRKGFFPKDFSKWLHGAFNLRQEADYAPL
                     RCPSKEEAHEVLDNAIRFLRGVKERLEKQLTNSSAQ"
     gene            complement(587..1063)
                     /locus_tag="PKH07_15180"
     CDS             complement(587..1063)
                     /locus_tag="PKH07_15180"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HNT36327.1"
                     /translation="MEQTKSRQRLRAFHHSSLIAFEERRWNAIVPEVLTLDTAPNIVV
                     IFKVTKGFDMSPDDRHVLEEFARNLRLRYPEALVWAFGSRARGDNRPESDFDVCVVLS
                     NASPENRSAVRSLAWETAFEHCLLLNVVVFAASDFERGPVSESSLVKSVLAEGVAA"
     gene            complement(1310..>1663)
                     /locus_tag="PKH07_15185"
     CDS             complement(1310..>1663)
                     /locus_tag="PKH07_15185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36328.1"
                     /translation="GCLWKNSGFSPPKILSEASRSAHYTESELLELDFEGSRPRLAEL
                     SAQWKEALSQAKDVVALLPLEEVGKCALDRRARLFRGDAAALEQALAQGQIQFHAGSI
                     RGAMPSFLNLEHRSS"
BASE COUNT          401 a          480 c          432 g          350 t
ORIGIN      
        1 aatcgcgcaa gcaatcttgg gcactcctga aatccggtct gccggcttac tgccgaagca
       61 tgcgaaaggg cgcagcaagc agcgccccca cacaactaaa agatgcgggt acggaaaccc
      121 gcgttgccag ggagatgccc catattcccg gttattggag gaaatctggc tgctcactgg
      181 gcagaggagt tcgtgagttg cttttccaga cgctctttta cgccgcgcag gaatcggatg
      241 gcattgtcca gcacttcatg cgcctcttcc ttggacggac atcgcaatgg agcgtagtcc
      301 gcttcctgac gcaaattgaa ggcaccatga agccatttgg agaaatcctt gggaaagaag
      361 ccctttcgaa cgtaatcccg atcaaaaagg gaaatgactc cctgatgctt ggaagactct
      421 ctttgctcaa ccgcgagaag tgcaagcaca gcatagaaca tcgcgtagta ggagaggtgt
      481 atgacttcgc gcatcgcacc ctcgttctcg agcagagtcg cagctcgaac agaactctca
      541 gcctgctcga tgcggaagtt gaccagagca gagatgtttt cctgagtcac gccgctacac
      601 cctccgcaag aacactcttg accaaggaac tctcggatac aggacctctt tcaaaatccg
      661 acgccgcaaa aaccacgaca ttaagaagga gacaatgctc gaaggctgtt tcccaagcaa
      721 gcgaacgtac tgcagaacga ttttctggcg acgcgtttga cagcaccaca caaacgtcaa
      781 agtcagattc tggacgattg tctccccgtg ctctggaacc gaacgcccag accaaggctt
      841 ccgggtagcg aaggcgaagg ttccgcgcaa actcttcgag aacgtgtctg tcgtcaggcg
      901 acatgtcaaa tcctttcgtc actttgaata ttacgactat gttcggagcg gtgtcaagcg
      961 tgagcacctc tggaacgatc gcgttccacc gtctttcctc aaacgcaata agagaagaat
     1021 ggtggaacgc cctcagtcgc tggcgcgact tcgtttgttc caccctaccc gttctgtggc
     1081 attgtggtgc gggcttccag cctgcagaca caagaagcag gcaagatgcc tgcaccacaa
     1141 tcgcgcaagc aagcttgcgc actcctaaat caggctctcg cggagtgcgg taacttgcta
     1201 ccgcgttctg gcttcatgct tcagtcgttc gtagttatgc cgtaaggcgt gatccggaat
     1261 atgcccttac gggcacacta cgaacccctc gcgcaagcga gattgcgccc tacgaactgc
     1321 gatgttccag attcagaaat gagggcatcg ccccacggat actgcccgca tggaactgga
     1381 tctgaccttg agccagagct tgttcgagtg cagcagcgtc tccccgaaaa agccgtgccc
     1441 tacgatccaa ggcacatttc ccgacctctt ccaagggcag gagagcaacg acatccttcg
     1501 cttgtgagag ggcttccttc cactgcgcag acagctcggc aagacgtggt cgagaccctt
     1561 cgaagtccag ttccagcagt tctgactcgg tatagtgcgc actccttgac gcttccgaga
     1621 ggattttcgg gggactgaat cccgagtttt tccacaggca gcc
//
LOCUS       DAOQUC010001023         1663 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_98012_length_1663_cov_2.639925, whole genome shotgun sequence.
ACCESSION   DAOQUC010001023 DAOQUC010000000
VERSION     DAOQUC010001023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1663)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1663)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1663
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98012_length_1663_cov_2.639925"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(50..958)
                     /locus_tag="PKH07_15190"
     CDS             complement(50..958)
                     /locus_tag="PKH07_15190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HNT36329.1"
                     /translation="MDASERTVTIGNVTLVGALINVALSTAKVLAGVYGHSQAVVADG
                     VHSLSDLATDVAVIIGVRYWSEPADGDHPYGHGRIETLITTVIGVTLFVVGVFIGYRA
                     FIGLSINDEFAPSWIAFWAAIASVVLKEWLYRWTIGVGRRIRSQALIANAWHHRSDAF
                     SSVPAVIAVATAILKPSFAKADHLGAMIVSGFIIAASWKIVSPCLQQLIDRGATLEDL
                     SRIHQFASEIHGVRSVHGLRTRYIGSSLAVDLHVLVDGDATVRTGHDIAREVEENLLE
                     RMPEVLDVVVHSEPEDHTSSSEYQQR"
     gene            complement(986..>1663)
                     /locus_tag="PKH07_15195"
     CDS             complement(986..>1663)
                     /locus_tag="PKH07_15195"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HNT36330.1"
                     /translation="DEKIEQGAQVYVIAPLIDESDAFPTLSTVRERHEHLAEIVFPHR
                     RVGLLHGRLSSEEKLQILGKFFRGELDVLVSTTVIEVGVDVARASVMLIESSERFGLA
                     QLHQLRGRIGRGQLESYCLLMTGSECSENARKRLKILEKTDDGFRIAEADLAMRGPGE
                     LAGYRQSGAQAFRIADLERDSQLLIEAWEDALRIVKEDPRLDQYQHRLLRRRLESLPQ
                     LQVVTIG"
BASE COUNT          420 a          482 c          378 g          383 t
ORIGIN      
        1 aatccttcag atcttccttg agtgtcctag cagacagaag gcagaaagtc tatctctgtt
       61 ggtactccga acttgatgtg tgatcctctg gttcgctatg gaccacaaca tccaacacct
      121 ctggcatccg ttccagcaga ttctcctcga cctctcgagc aatatcgtgt cccgttctga
      181 ccgtcgcatc accgtccaca agcacatgca aatccactgc cagactgctt ccgatatagc
      241 gggttctcaa accgtgtaca gatctgaccc catgtatctc tgatgcaaat tgatgaatac
      301 gggaaagatc ctcaagcgtc gctccccgat caataagctg ctgaaggcat ggcgagacaa
      361 tcttccagga tgccgcgatg atgaaaccgg acactatcat cgcgccaaga tggtcagctt
      421 tggcaaagga aggtttgagg atggctgtcg ctacggctat cacggctgga acagaactaa
      481 acgcatccga gcgatgatgc cacgcattgg cgataagtgc ctgtgaacgt atccgacgcc
      541 ccactccgat cgtccaacgg taaagccact ccttcagaac aacggaagca atcgcggccc
      601 agaaagctat ccacgacggt gcaaattcat cgtttatcga aagaccgatg aaagctcggt
      661 aaccgatgaa tactccgaca acgaaaagcg taacaccaat gaccgtcgta atcagtgtct
      721 caattcggcc atgcccgtaa gggtggtccc cgtcggcagg ctcagaccag taacgaactc
      781 cgatgatcac ggccacgtca gtcgccaaat ccgaaaggct gtgaactcca tcggcaacaa
      841 cggcctggct gtgaccgtaa actccggcga gaacctttgc ggttgagagg gctacgttga
      901 tcaaagcgcc caccaaggtc acatttccta tcgttacggt cctttctgaa gcatccatat
      961 cagcctcctg tcaatgcgct tcactctatc ctatggtcac gacttggagt tgtggaagcg
     1021 attcaagacg acgacgcaag agcctgtgct ggtattggtc aagtcttgga tcctccttca
     1081 caatcctgag tgcatcttcc caggcttcga tgagaagctg actatctcgt tcaaggtcag
     1141 caattcgaaa tgcctgggct ccactctggc ggtatcctgc cagttctccc ggacctctca
     1201 ttgcaagatc ggcttcagca atacgaaaac catcatccgt cttctcaaga atcttgagtc
     1261 tcttgcgtgc attctcagag cactcactgc ccgtcatcaa gagacaatac gattcgagtt
     1321 gacctcttcc aattcgcccc cgcagctgat gcagttgtgc caaaccaaac cgctctgaac
     1381 tttcaatgag cataactgag gctcgtgcca catctacgcc gacttcaatc actgtcgtag
     1441 agacgagaac atcgagttct cccctgaaga actttccgag tatctgcagt ttctcttcac
     1501 ttgaaagcct cccatggagg agacctactc tccgatgagg aaagacaatt tcagccagat
     1561 gttcatgtcg ctcacgaaca gtgctcagcg ttggaaaggc atccgattcg tcaatgagcg
     1621 gtgcaatcac gtagacctga gcgccctgct cgatcttctc atc
//
LOCUS       DAOQUC010001024         1662 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_98161_length_1662_cov_1.801493, whole genome shotgun sequence.
ACCESSION   DAOQUC010001024 DAOQUC010000000
VERSION     DAOQUC010001024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1662
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98161_length_1662_cov_1.801493"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /locus_tag="PKH07_15200"
     CDS             complement(<1..500)
                     /locus_tag="PKH07_15200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HNT36331.1"
                     /translation="MKSRKIPQSQHTRLRSLSWRRISGNGFFYLVVFGIALLIVLPLL
                     WVLSSSLKTPEQVFDISSQWIPRPIQWANYIRAFQSMPVLLYTRNTVIITSLCILGYL
                     LSGSLVAYAFSRLRWPGRDLFFMILLGTMMLPAQVTIIPLFVLFRNLGWLNTYYPLIV
                     TTYLTG"
     gene            complement(502..1470)
                     /locus_tag="PKH07_15205"
     CDS             complement(502..1470)
                     /locus_tag="PKH07_15205"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNT36332.1"
                     /translation="MRVPEHSPAPVSAKKVCRIAKKSRFGRETGLGYLFASPWLIGFV
                     VFGLYPIGMSIYYSLCRYDVLRPPMFVGLANYRYLFAESSTFWHSIRNTLVFTVFRVP
                     LVILGSLLLAMLVERQRPGIRLARTLYYLPSIISGVSLSVIWLWLYNPKHGLINQFLM
                     HFGIQTPLWLESPTWSKPALILMGLWSIGGGRMILFIAGLNSVPRQLYECALLDGAGW
                     WHRFRHITLPQIAGILFLLTVAEMIGSFQVFTESYIMTRGGPSNSTLFYNLELYNRAF
                     LDYQMGLASAMAWLLFLGTMAVTTALFRWVGTRIYYEASGADGKRF"
     gene            complement(1467..>1662)
                     /locus_tag="PKH07_15210"
     CDS             complement(1467..>1662)
                     /locus_tag="PKH07_15210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36333.1"
                     /translation="LAHTVFWREYSRAWDDAVRGQFSARQALERAQREIQSVLDDQLE
                     YNDYYLRVKGRKSSMEQRSR"
BASE COUNT          467 a          396 c          447 g          352 t
ORIGIN      
        1 caaccggtca ggtatgtagt gacaatcaag ggatagtatg tgttcagcca acccagattt
       61 ctgaacagga caaacagtgg gatgattgtc acttgtgcag gcagcatcat cgtgcccaga
      121 aggatcataa agaagagatc acgccctggc cagcgcaaac gggagaaggc ataggcaacc
      181 agcgacccag atagtaggta tccgagaatg cagagactcg ttatgatgac tgtatttctc
      241 gtgtacaaga gcacaggcat gctttgaaaa gcgcgaatgt agtttgccca ctgaatcggg
      301 cggggaatcc actggctgga gatatcgaag acctgttcgg gtgtcttgag tgagctagaa
      361 agaacccaga gtagaggcag cacaatgaga agggcgatac cgaagactac aagatagaaa
      421 aagccatttc cggaaatgcg tcgccagctg agactgcgaa gtcgcgtgtg ctggctctga
      481 ggtatctttc tactcttcat tctagaacct tttgccgtct gcgcctgatg cttcgtaata
      541 gatgcgcgta ccgacccacc gaaacagggc agttgtcacc gccatagttc ccaggaagag
      601 aagccatgcc atggcggaag ccagtcccat ctgataatcg agaaaagctc tgttgtagag
      661 ttcgagattg taaaagagtg ttgagttgga cgggccacct cgcgtcatga tgtatgactc
      721 ggtgaagact tggaacgatc caatcatctc ggcgacggtg agcaggaata ggatgcctgc
      781 gatctgaggc agcgtgatgt gacggaaccg atgccaccat cccgcgccat ccaagagtgc
      841 acattcatag agttgtcgag gaactgagtt caagccggcg atgaaaagga tcatcctacc
      901 accaccgata gaccatagac ccataaggat cagcgcaggc tttgaccagg ttggcgattc
      961 gagccagagt ggagtctgaa tgccaaaatg catgaggaat tggttgatca atccatgctt
     1021 ggggttgtag agccagagcc agataacaga gagcgagact ccagagataa ttgatggcag
     1081 atagtagagc gttcgggcaa gcctgattcc aggacgctgc cgttctacca acattgccaa
     1141 gagcaaggaa cccaagatca ccaaaggaac tcggaatacg gtgaaaacta atgtattcct
     1201 tattgagtgc cagaaagtcg aagattcggc gaagagatag cggtagttgg ccaacccaac
     1261 gaacatcggt ggacgtaaaa cgtcatagcg acacaggctg tagtagatag acatcccgat
     1321 tggatagagt ccgaagacga caaatccaat aagccatggg ctggcaaaca agtaccccaa
     1381 tccggtctct ctaccgaatc tggacttctt cgctattctg cagactttct ttgctgaaac
     1441 aggtgccgga ctgtgctcag gaactctcat cgggatctct gctccatgga agatttcctg
     1501 cctttcaccc gcaggtaata gtcattgtac tccagttgat catcgagaac cgactgtatc
     1561 tctcgttgtg cacgctccaa agcttgtctg gcagaaaatt gcccacgaac ggcgtcgtcc
     1621 caagcgcgag agtactctct ccagaatacg gtgtgtgcca aa
//
LOCUS       DAOQUC010001025         1658 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_98471_length_1658_cov_3.658765, whole genome shotgun sequence.
ACCESSION   DAOQUC010001025 DAOQUC010000000
VERSION     DAOQUC010001025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1658
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98471_length_1658_cov_3.658765"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..635)
                     /locus_tag="PKH07_15215"
     CDS             complement(<1..635)
                     /locus_tag="PKH07_15215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36334.1"
                     /translation="MSYGQTRGHWAIALVLAAVVLTGGTAWAVDGQIDIYPTGATPYV
                     ISSPGSYVLVTDVNMTADVDCIQIAASNVTLDLNGHTLTGTGTGNSSGVMQTTGVGAL
                     VFNGSIYNFGRHGIFLNERSQVSDLSVLNCGDDGGLYYHAIRVGEGSIISRVTAANNY
                     GNGIDAASNCLVESCVARNNGTVSHKGIAVGSGSTVKDCIAGNNGHRSSQDS"
     gene            1227..>1658
                     /locus_tag="PKH07_15220"
     CDS             1227..>1658
                     /locus_tag="PKH07_15220"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HNT36335.1"
                     /translation="MRIYRSRKLTSEVLCLLLLVGGVLTAGCTKEQGTPQVKQSHSDM
                     ISLAVGTFGASEDTPMDVVRSLNDLFSDALADTGKFNLLERARMKDIEEEYLRMHGDR
                     SAVSAADPERGDDITFLTALLSNDSSLWVNMERGKKLRASDQ"
BASE COUNT          381 a          431 c          434 g          412 t
ORIGIN      
        1 gaatcctgtg aactcctgtg tccattgttc ccggcgatac aatccttgac cgtcgaaccg
       61 ctgccaactg caatgccctt gtggctgacc gttccgttgt tacgagctac gcaggattca
      121 accaaacaat tcgaggcagc gtcaattcca tttccgtaat tattcgcggc ggtgaccctg
      181 gaaatgatgg aaccttctcc tactcgaatg gcgtgatagt agagtcctcc gtcatcgccg
      241 cagttgagaa cagacagatc gctgacttgg ctgcgttcat tgagaaagat gccgtgtctg
      301 ccaaagttgt agatcgaacc attgaatacc agcgccccca cgcctgtcgt ctgcattacc
      361 ccgctgctgt ttccggtgcc agttccggtc agcgtgtgac cgttcaagtc gagcgtcacg
      421 ttcgatgcag caatctggat acagtccaca tcggcagtca tattcacatc cgttaccaga
      481 acatacgacc caggtgaact gatcacatag ggagtcgctc cggtgggata gatgtcaatc
      541 tgcccatcca cagcccaagc cgtgccacct gttaggacaa ccgccgccaa gaccagtgct
      601 attgcccaat gccctctcgt ttgaccataa gacatgttgg actcctttgc ccttttttgc
      661 cctgatgtct cgatcaggaa atggttgagg caagagtgtg acctgcctgt ttttgccgat
      721 tccgctattc agaagacttg cttctgtcca aaccgccaca aatcattggt atcaacaatc
      781 caacacaccc atttacccta acagaatcgc tccgaatccg tcaatgattt gtcgcctttc
      841 atcgtgtcgg ctcagttacg gaggggggca ggcgagctcc cgtgtaagtg agatgtcact
      901 ctgagcggag tctgcgcggc gaagagtctc tctgtgcctt tgctgacggg cttcgggctg
      961 caggagagat tcctcactcc ttcgccaagc ctcggtcgtt cgggatgaca gtttccgccc
     1021 ttgtgtaact gagtaaatac ctttcattct tgacaaagtt tggatcacgg tgacatgatc
     1081 tattgacccg tctcgggaaa ctgatcttgg tgttctcgga tgaatatggg acagcctcat
     1141 ttcgtcttac tgcatgagtt ggagagccgt attctatgtc cgggaaagat ggaaccatta
     1201 ggaatcggat aagaaaggac agaacgatga gaatctatcg gtcgagaaag ttgacttcgg
     1261 aggtgctctg tcttctgctg ttggtggggg gtgttctcac cgcgggatgc acaaaggaac
     1321 agggaacgcc acaggtgaaa cagagtcatt cggatatgat ctcgctggca gttggaactt
     1381 ttggcgctag tgaagacacg cccatggatg tcgttcgaag tttgaacgat ctgttctccg
     1441 atgcgcttgc cgatacaggg aaattcaacc ttcttgagcg agcacgcatg aaggatattg
     1501 aagaggagta tctccgaatg catggagata ggtctgccgt ctcagccgcc gatccggaac
     1561 gcggtgacga catcaccttt ctgacggcgc tgctgagcaa tgacagtagt ctttgggtca
     1621 atatggaacg cggcaaaaag cttcgggcga gcgaccag
//
LOCUS       DAOQUC010001026         1657 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_98604_length_1657_cov_3.059925, whole genome shotgun sequence.
ACCESSION   DAOQUC010001026 DAOQUC010000000
VERSION     DAOQUC010001026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1657)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1657)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1657
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98604_length_1657_cov_3.059925"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..768)
                     /locus_tag="PKH07_15225"
     CDS             complement(<1..768)
                     /locus_tag="PKH07_15225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HNT36336.1"
                     /translation="MNRKAFALFVYLCNAVAMAQSSCPSPRIDFSMTFDGEAILLHGG
                     YSWDETMQSPMLMDDLWRWNGLVWAETEPSLRPARRLRHASCFDSVRNEVVLFGGMLE
                     DWNYAKDVWIWDGTNWFENTGDPGILRGEGHLSFDSARGVSVLYVGDIFTERTSQTWE
                     FDGSEWTQRLTVDSPGPRLDTGFVYDASRQVCVLFGGVVRDERGADSANDETWEYDGI
                     NWSKRDLDLSPSPRFGFSMAYDSERARVVLFGGQLDTG"
     gene            complement(882..>1657)
                     /locus_tag="PKH07_15230"
     CDS             complement(882..>1657)
                     /locus_tag="PKH07_15230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020492186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbamoyl phosphate synthase large subunit"
                     /protein_id="HNT36337.1"
                     /translation="RELHVVGLMNMQYAIANDTVYVLEANPRASRTVPLVSKVCGISM
                     ASIATQVMLGKRLSDMNLSTRTVPHFGVKEAVFPFNMFPEVDPLLGPEMRSTGEVLGM
                     AGSFGFAFYKAQDAAKQTLPSEGTVLITVAKEDQPPVLEAARKFYDMGFKIVCTSGTR
                     QFLAEHGIAADVIMKMNEGRPNIVDAIKNGEIHLVVNTPTGRVGKYHDSYIRKAAIKY
                     KVPYITTTAGALAAAKGIESYRKESWSVRSLQSYHKDLR"
BASE COUNT          416 a          479 c          398 g          364 t
ORIGIN      
        1 acccgtatcg agttgcccac caaagagaac aactctggct ctctcactat cgtaggccat
       61 cgaaaagcca aagcgcggac tcggtgagag atctaaatca cgcttgctcc agttgatgcc
      121 atcgtattcc cacgtctcgt cgttcgcgga atctgcaccc ctttcgtccc gcaccacgcc
      181 accaaacaaa acgcagactt gcctgctggc atcgtaaaca aatccggtgt cgagacgagg
      241 ccccggtgaa tccaccgtga gtcgttgcgt ccattcgctg ccgtcaaatt cccatgtttg
      301 actcgtacgc tcagtgaaga tgtctccaac atagagaaca gagacgcctc tcgcactgtc
      361 gaaggaaaga tgcccttccc ctctcagaat cccgggatcg cctgtgttct caaaccagtt
      421 cgttccatcc cagatccaga catctttcgc atagttccag tcttcaagca tcccgccaaa
      481 cagaacaact tcattacgaa ccgagtcgaa acaggaagcg tggcgaagac gacgcgcagg
      541 acgaagagac ggttcagtct ccgcccacac gaggccgttc catcgccaaa gatcatccat
      601 aagcatgggg ctctgcatgg tctcgtccca gctgtagcca ccgtgaagca agatggcctc
      661 tccatcgaaa gtcattgaga agtctattcg gggagaagga caggaagact gagccatcgc
      721 gaccgcatta caaaggtaaa caaacaaggc aaaggctttc cgattcatcg catgcctcct
      781 gcaagacccc aatccgcgcc gctcagcaaa ggctcacgat ccagactcca gtatgatccg
      841 gacgcgtctg gcaagatcgc tctgcactag ttgacggtac gctagcgcaa gtccttgtga
      901 taagactgaa gtgatctcac actccaagac tctttccgat aggattcgat ccccttggcg
      961 gcggccagag cacctgcagt cgtcgtaatg tagggcacct tgtatttgat cgcagccttg
     1021 cgaatgtagg agtcgtggta ctttccaact cggccagtcg gagtgttcac cacaaggtgg
     1081 atctcaccat tcttaatcgc gtcaacgata ttcggtcttc cttcattcat cttcatgatc
     1141 acatccgcag cgataccatg ctcggcaagg aactgacgtg tacctgaagt gcagacgatc
     1201 ttgaaaccca tgtcatagaa ttttctcgcc gcttccagaa ctggaggctg atcttccttt
     1261 gcgaccgtga tcagaactgt accttcagag ggaagagtct gtttggcggc atcctgagct
     1321 ttgtagaaag caaatccaaa agatcctgcc atcccaagaa cttctccagt cgagcgcatc
     1381 tccggaccga gcagtggatc cacttcggga aacatattga acgggaagac cgcttctttg
     1441 accccaaaat gaggtaccgt gcgagtggaa aggttcatat ctgacaggcg tttgccaagc
     1501 atcacttgtg tcgcaatgct agccatcgaa atgccacaca ctttggagac gagaggcact
     1561 gttctggaag cccttggatt ggcttccaag acgtagacgg tgtcgttggc aatcgcgtac
     1621 tgcatgttca tcagaccgac aacgtgaagc tcccggg
//
LOCUS       DAOQUC010001027         1656 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_98698_length_1656_cov_3.329794, whole genome shotgun sequence.
ACCESSION   DAOQUC010001027 DAOQUC010000000
VERSION     DAOQUC010001027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1656)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1656)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1656
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98698_length_1656_cov_3.329794"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1638)
                     /locus_tag="PKH07_15235"
     CDS             complement(<1..1638)
                     /locus_tag="PKH07_15235"
                     /inference="COORDINATES: protein motif:HMM:NF023649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3604 domain-containing protein"
                     /protein_id="HNT36338.1"
                     /translation="MPIGEPGSAYSPTIVCDWEDRLHVVWTSCHEQVWHLQYAVLQDG
                     KQSLSRTLSKGGSSDRYPSLSVTSGGEIWLAWESDVRGNRDVYAGRLTSNGLQDITPM
                     STDDSEDCAPSLTASPKGGLGLAWHRYLGNGNFDIFFREKDKQEWKPEVRLTDFPAVD
                     AFPSIAADSKGRFWIAWHSNRLGEVSGITNWILVRCWDGKNFLETVNPMPGVDLEKDH
                     TDQGLEFPTIHVDQRDRVWIFGRPSHNFCAQVYSGDSWSPLIRLPKDGWGGRGQFIRV
                     AEDPSGKLWLARRDIRENMLQEISDISKQAEPLKLKRAKGTSSKSAKSTLAGISKTPY
                     RVGEYNLYFGDIHAHTWMSDGMGSLDEFWTRCRDVLEWDFAALTDHDDFVGNRISPAE
                     WEEMKYVANRYDDPGRFAAIHAYEWTTGRPPSGFGHKNVYFSDTNPPLFRKCDADAAS
                     PSLLYEKMKPWNALAFPHHIAWTGSDWEHLDPQISTCVEIISNHGRFEFMGNLPIRHR
                     GGRQGEFVQDGLKQGLRFGIVGGSDSHGLRWQHGVSYK"
BASE COUNT          382 a          474 c          425 g          375 t
ORIGIN      
        1 tttgtaactg acgccgtgct gccatcgcag tccgtggctg tcactgcctc caacaattcc
       61 aaatcggagt ccttgcttca gcccgtcctg cacgaattcc ccctgcctgc cccctcggtg
      121 ccggatggga aggttgccca tgaactcaaa acgaccatga ttggagatga tctccacaca
      181 ggtggaaatc tgaggatcca agtgctccca gtcggagccc gtccacgcga tatggtgtgg
      241 aaaagccagt gcgttccatg gcttcatctt ttcatagagc aaagatgggg aggccgcgtc
      301 ggcatcgcat tttcggaaca gaggcgggtt tgtgtctgag aaataaacgt tcttatgacc
      361 aaatccgctg ggcgggcgtc ccgttgtcca ctcataagca tgaatggcgg caaaacggcc
      421 tgggtcgtcg tagcgattgg caacgtattt catctcctcc cattcggcag gggaaattcg
      481 attgccaacg aagtcatcgt gatcagttaa agcagcaaaa tcccactcca aaacgtcccg
      541 acagcgcgtc cagaattcat ccaacgaccc cattccatcg gacatccatg tgtgagcgtg
      601 aatatccccg aaatagagat tgtactcacc aacgcggtag ggtgtcttgg aaattcccgc
      661 taaagttgac ttggcagatt ttgaggacgt tcctttggcg cgtttgagtt tgagtggctc
      721 tgcttgcttc gaaatgtctg aaatctcctg aagcatattc tcacggatgt cgcgtcgtgc
      781 cagccacagt tttccggaag gatcttcggc aacgcggatg aactggccac gaccgcccca
      841 accatctttg ggcagtcgaa tcaagggaga ccagctgtca cccgaataga cctgtgcaca
      901 gaagttgtgc gacggccgtc cgaaaatcca gacgcggtct cgctggtcaa catgaatcgt
      961 cgggaattca agtccctgat ccgtgtggtc tttctccaaa tccactccgg gcatggggtt
     1021 gactgtttcc aagaagttct tcccgtccca acagcgaacc aatatccagt tggtgatgcc
     1081 agaaacctct cccaaacggt tgctatgcca cgcaatccag aaacgcccct tgctgtcagc
     1141 ggcgatagaa ggaaaggcgt ccacggcagg aaagtctgtg agacgaacct ccggtttcca
     1201 ctcctgcttg tccttctccc gaaagaagat gtcgaagttg ccattgccca ggtatcggtg
     1261 ccaggccaaa cccaaaccgc ctttgggact tgccgtgagg ctcggagcac agtcttcgga
     1321 gtcgtcagtg ctcattgggg tgatgtcctg caagccgttt gaagtgagtc gtcctgcgta
     1381 cacatctcga ttgccccgta catcggactc ccacgcaagc cagatctctc caccagaagt
     1441 cacagagaga gacggatacc gatctgatga gccgcctttc gagagtgtcc gcgagagact
     1501 ctgttttccg tcctgcagga ctgcgtactg aagatgccag acttgttcat ggcaacttgt
     1561 ccagaccaca tggagccgat cctcccagtc acaaactatc gtcggtgaat aggcactacc
     1621 tggctcaccg atcggcattt ccggcaacca atgacc
//
LOCUS       DAOQUC010001028         1656 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_98749_length_1656_cov_2.083698, whole genome shotgun sequence.
ACCESSION   DAOQUC010001028 DAOQUC010000000
VERSION     DAOQUC010001028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1656)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1656)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1656
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98749_length_1656_cov_2.083698"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1656
                     /locus_tag="PKH07_15240"
     CDS             <1..>1656
                     /locus_tag="PKH07_15240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36339.1"
                     /translation="GDYKIKGPDGGTLTATRTLSRDYYQNVSDLVDVNIQLDPVLDQP
                     KPRSIFVEEKYDANVFELKEGGFISYSQFVTTRVEGGWGYLVWQFDNYRTAMPDLVNL
                     VYTLTVKNRATGPSYWEGHWESPTGENGAIIGDNQIIPLVSGQPEPTPVQGRSCRRTF
                     VADPKVYRPGQPMNVKLEFVTGAKVRTLSVTERIPEGWTYLSSSSTVAPVTGPFVLGR
                     SITWPFGSPSQLDDISITYRVVPDAESVGVQIFSGVWAYQEETLSDYVDYFVTEGDQT
                     ITDGLTGLIPGLIDMTRDLPSTYTGNSTVDVSIAYDIAEASPPTQVTIIENIPKGWEL
                     VSAHPPVSASTTMSLRWNLGVDPIQRVVLDGSVFYTLRVPFGEFGEKAFNGSYTASTG
                     AAPIGGLIYGDSRVQGDMGGCPLFVSEGGNVPGRIDAWELLSLFDRIRSGSEEGEMLF
                     CFQRQWYQQEPVYNTPTPTQTEGVPTETPIPATVTPTETQVPSTFTPTDTSVPATATP
                     TQTQVPATETPTDTSVPATATPTETSIPATATPSETSVPPTST"
BASE COUNT          387 a          428 c          445 g          396 t
ORIGIN      
        1 caggcgatta caagatcaaa gggcccgatg gtggaacatt gacagccacg agaactctgt
       61 cgagagacta ctatcagaac gtgagcgatc tcgtggacgt gaacattcag ctggatcccg
      121 ttcttgatca gcccaaaccg cgttccattt tcgtggagga gaagtacgac gcgaatgttt
      181 tcgaactgaa ggaaggtgga ttcatcagtt actcccagtt cgttacgact cgggttgaag
      241 gaggctgggg ctatctggtt tggcagtttg acaactatcg gacagcaatg ccggatctgg
      301 tgaaccttgt ctatactctg acggtcaaga atagggctac gggaccctct tactgggagg
      361 ggcactggga atctccaact ggagagaacg gtgccatcat tggggacaat cagatcatac
      421 ctttggtatc tgggcagcct gagccgactc cggttcaggg acgttcttgc agacgaacgt
      481 ttgtggcgga tccaaaggtc taccgacctg ggcaacctat gaacgtcaag ttggagttcg
      541 tgactggagc caaggtcagg acgctttctg tgacagagag gattcccgaa ggatggacgt
      601 atttatcttc aagttcgacc gttgcacctg tcacgggtcc ttttgtcctg gggcgttcaa
      661 ttacctggcc gttcggatca ccttcgcaac ttgatgatat ttcgattacc taccgggttg
      721 tgcccgatgc ggaatctgtt ggtgtgcaga ttttttctgg tgtctgggca tatcaggaag
      781 aaaccctatc ggattatgtt gactatttcg tcacagaagg tgaccagacg atcacagatg
      841 gactgaccgg cctcattccg ggtctgatag atatgactcg ggacttaccc agtacctaca
      901 ctggaaactc tacggttgac gtaagcattg cctatgacat cgctgaggcc agccctccga
      961 ctcaagtgac tatcattgaa aacattccaa agggctggga actcgtctca gcacatccac
     1021 cggttagtgc gtcaacaaca atgtctctgc gatggaatct tggtgtagat ccaatacaac
     1081 gggtcgtgct tgatgggagt gtattttaca cacttagagt tcccttcggt gaattcggtg
     1141 agaaagcgtt caacggttcc tatactgcga gtactggtgc agcgccaatt ggcggcctga
     1201 tctatggcga ttcccgcgtt cagggagaca tgggaggctg tccgctcttt gtcagcgagg
     1261 gcggaaacgt tccgggtagg atagatgctt gggaactgct gtcgcttttc gatcggattc
     1321 gttctgggtc agaggaaggc gagatgctgt tttgtttcca gcggcagtgg tatcagcagg
     1381 agcccgtcta caacacaccc actccaaccc agactgaggg agtgccaaca gaaactccca
     1441 ttccggcaac agtgaccccc actgagaccc aagttccttc gacgtttacg ccaactgata
     1501 cttccgttcc tgcgaccgcc acacccaccc agacacaggt tcctgctacg gagacaccga
     1561 cagatacttc tgttcctgcg accgccacac caacggagac ttccatccct gcaacggcga
     1621 cacccagtga aacatcagtt cctccgacga gcactc
//
LOCUS       DAOQUC010001029         1655 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_98813_length_1655_cov_2.873125, whole genome shotgun sequence.
ACCESSION   DAOQUC010001029 DAOQUC010000000
VERSION     DAOQUC010001029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1655)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98813_length_1655_cov_2.873125"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1655
                     /locus_tag="PKH07_15245"
     CDS             <1..>1655
                     /locus_tag="PKH07_15245"
                     /inference="COORDINATES: protein motif:HMM:NF016859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36340.1"
                     /translation="QVWFALSRSWNDAIKPRAIVDLDRQQTLKALIEKDRKYKQEDKE
                     FFRPLLRQFANIDEARIAYDNGIIGLQEEIRIRVKNETIKTTMGRVFFNEALPENFPF
                     VNKLMDKKELSRLVSRIYFESGQAETALLLDRLKTLGFTYAKLSGLSISVSDLVVPAE
                     KRGIIAQADEDVRRVMEQFRQGVITDVERYNKIVDTWQRTTYDVGAFMMKRFEKDQCG
                     FNPIFMILNSASRGSRDQISQLAGMRGAMNRPTKKMTGAVGEVIESPIKSNFKEGLTM
                     LEYFISTHGARKGLADTALKTAEAGYLTRRLVDVAQDVIVNEDDCGTMQGVEVGAIRA
                     GDLVFEGLKDRIVGRVSLDDIYNPLSSEREVLVAAGEIITPEIADRIVLCGIEHVTIR
                     TVMTCETRRGICAKCYGWDMTRGKLVEVGEAVGVIAAQSIGEPGTQLTLRTFHIGGTT
                     SRVLRQSDARVSKFEQDGVGFRLGEFGHVKFINVKTVDDRKGTRTVINRAGRILAARS
                     GIRADGVAKGTTGRVIYKNIAIEKDSEGRSIVLNSDGSLELQY"
BASE COUNT          413 a          380 c          463 g          399 t
ORIGIN      
        1 caggtttggt ttgcactctc ccgatcttgg aacgatgcca ttaagccaag ggctatcgtg
       61 gatctcgaca ggcagcagac gctgaaagct cttatagaga aagaccgcaa gtataagcag
      121 gaagacaaag agtttttcag gccccttttg aggcagtttg cgaacattga tgaggctcgg
      181 atcgcgtacg acaatggaat catcggccta caagaggaga ttcgtatccg agtcaagaat
      241 gaaacgatca agaccaccat gggaagagtc tttttcaatg aagctcttcc tgagaatttc
      301 ccatttgtca ataagttgat ggacaagaag gaactctctc gcctcgtgtc ccgcatctac
      361 tttgaatccg gccaggctga gacagcactc ctcttggatc gcctgaagac cttgggcttc
      421 acctacgcga agctttcagg actctctatc agtgtttctg acttggttgt gcctgcggag
      481 aagcgtggca tcatcgccca ggcggatgag gatgttcgtc gagtcatgga gcagtttcgc
      541 cagggtgtca tcactgatgt cgaaagatac aataagatcg ttgatacctg gcagcgaaca
      601 acttacgacg tgggtgcttt catgatgaag cggttcgaga aggatcaatg cggattcaat
      661 cccattttca tgatcctgaa ttcagcatca cgtggttcca gagatcagat tagccagttg
      721 gcggggatgc gtggtgcgat gaaccgtccc accaagaaga tgacaggtgc cgtcggagag
      781 gtcattgaat ctccgattaa gtccaatttc aaagaaggac ttaccatgtt ggagtatttc
      841 atctccaccc atggcgctcg aaagggtctt gccgacaccg cgttgaagac cgcagaagcc
      901 ggatacttga cccgtcgcct tgttgacgta gcgcaggacg tcattgtgaa cgaagatgac
      961 tgcggaacga tgcagggcgt tgaggtaggt gccatccgcg ctggagacct tgtgtttgag
     1021 ggtctcaagg atcgtatcgt tggtagagtc tccttggatg atatctacaa tccgctctct
     1081 tcggagcgtg aggtactggt cgccgcgggt gagatcatca cacccgagat cgcggacaga
     1141 attgttctct gcggtatcga acatgtcact atccgaacgg ttatgacctg tgaaactcgc
     1201 cgaggcattt gtgccaagtg ttatggatgg gatatgaccc gaggcaagtt ggtggaagtg
     1261 ggagaagctg tcggagtcat cgctgcccag tctattggtg agccgggaac acagttgaca
     1321 ttgagaacct ttcacattgg tggaacaacc agccgtgtgc ttcgtcagtc tgatgcccgt
     1381 gtaagcaaat ttgagcagga cggagttggt ttccgcctag gtgaatttgg ccatgtgaaa
     1441 ttcatcaatg tgaagaccgt tgatgatcgc aaaggcacca ggactgttat caaccgtgca
     1501 gggagaatcc tggcagcacg ctccggcatt cgagccgatg gtgtggccaa gggtacaacg
     1561 ggtagagtga tctataagaa cattgcaata gagaaagact cagaaggtcg ctctattgtc
     1621 ctcaactcgg atggctccct tgaactccag tataa
//
LOCUS       DAOQUC010001030         1655 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_98830_length_1655_cov_2.393750, whole genome shotgun sequence.
ACCESSION   DAOQUC010001030 DAOQUC010000000
VERSION     DAOQUC010001030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1655)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98830_length_1655_cov_2.393750"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1655
                     /locus_tag="PKH07_15250"
     CDS             <1..>1655
                     /locus_tag="PKH07_15250"
                     /inference="COORDINATES: protein motif:HMM:NF021126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HNT36341.1"
                     /translation="LFTFYFLLAYACWQALSSRIQALKARQRLTLISIVPLCFAGALL
                     LAAVILLPLVEVSLFTAARQGGASYHFATTDSLPPKHLITFLAPVFFGHPADDSFWET
                     TTGYHEICGYMGILPLILASVVLIRRPGRPFFVATAVASVVFAMGSFTPAFRILYHLI
                     PGFVWFRVPARLLLIYSFSMAVLAAMGTADLVEREAKPIGRTTKILWGSLVAVLVVVA
                     VVLSIGRPRVEEHLADQQKQREIQYSQESGEPFSPSPEWEQETRAACVKRFETMRSSV
                     HQALLWTALSLFAVILYIKGKQSPALLGGLLLLVADLGLFNYGLLPYQDRDYYDCTAF
                     AETETVRLLKSDGELFRVLTMDSAIGFLTRKTHPEIYPERLTVHGIQSVRGYNPIVLK
                     HYAAHINRMQGFPADQPVGGLLFLPLEQRLSRQALNLMNVRYLLTYRGAPPGFDLVLQ
                     DSGLSVYRNRQEHPRAYIVRNNALTRDPVEIEEYNANSVTLKTKLDEAGVLVLADTWY
                     PGWKALINGQKTDVEPFNEVFRAVLLEPGEHEVTFVFKPKSFF"
BASE COUNT          338 a          450 c          434 g          433 t
ORIGIN      
        1 gtcttttcac tttctacttt ctgcttgcgt acgcctgctg gcaggctctc tcttcgagaa
       61 ttcaagcctt gaaagcccgt cagcgcttga ccctgatctc aatcgttcct ctctgctttg
      121 cgggagcttt gctgcttgcg gcggtgattc tgcttccctt ggtggaagtc tctctgttta
      181 ccgcggcacg acaaggtggc gcgagttatc attttgccac cacagattca ttgccgccca
      241 aacatctcat cacctttctt gcaccggtgt ttttcgggca tcctgctgat gattctttct
      301 gggagacaac aaccgggtat catgagattt gtgggtacat gggaattctg cctctgatac
      361 ttgcttcagt ggtcttgatc cgaagaccgg gaagaccctt ctttgtcgca actgcagtgg
      421 cttctgttgt gtttgcgatg ggctcgttca cacctgcatt tcgaattctc tatcacctga
      481 ttccaggatt tgtgtggttt cgggtaccgg cacgactcct gttgatttac tcattcagca
      541 tggcagtgct ggctgcgatg ggcacggcgg acttggttga gcgcgaggcc aagcccatcg
      601 gaaggacaac aaagatactc tggggctccc ttgtggctgt acttgtggtt gtggctgtag
      661 tgctctcaat cggccgccct agggttgagg aacatcttgc cgatcaacag aaacagagag
      721 agattcagta cagccaggag tccggagagc ccttttcccc atcgccagaa tgggaacagg
      781 aaactcgcgc cgcatgtgtc aaacgcttcg agacgatgcg ctccagcgtt catcaggcgc
      841 ttctctggac ggcgttgagt ctctttgcag taattctcta cataaaggga aagcaatctc
      901 cggctctgct tggtgggctt ctcttgctgg tcgcagatct ggggcttttc aactacggtc
      961 ttcttcccta tcaagatcgg gactactatg actgcactgc cttcgctgaa acggagaccg
     1021 tgaggctgct gaaatctgat ggggaactgt tccgagtcct tacgatggat agtgccatcg
     1081 ggtttctgac aagaaagact catcccgaga tctatccgga gcgcctgacc gtccacggca
     1141 tccagagtgt tcggggctac aacccgattg ttctgaaaca ctacgcagca catatcaaca
     1201 gaatgcaggg gttccccgcc gatcaaccgg taggcggact tctttttctg cctcttgagc
     1261 aacgactcag caggcaggct ctgaatctga tgaacgtgcg ttatctgctg acctatcgag
     1321 gtgcgccgcc aggtttcgat cttgtgctcc aagacagtgg tctttccgtt tatcgcaacc
     1381 gtcaagaaca cccgcgagcc tatatcgttc gcaacaacgc gctcacacgc gacccggttg
     1441 aaattgagga atacaacgcc aactccgtca ctctcaagac caagttggat gaggcgggcg
     1501 ttctcgtctt ggcggacacc tggtatccgg gctggaaggc actgatcaac ggtcagaaga
     1561 ccgatgtcga gcctttcaac gaagtcttca gagccgtcct gctggaaccc ggtgaacatg
     1621 aggtgacctt cgtcttcaag ccgaaaagtt tcttc
//
LOCUS       DAOQUC010001031         1654 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_98923_length_1654_cov_2.533458, whole genome shotgun sequence.
ACCESSION   DAOQUC010001031 DAOQUC010000000
VERSION     DAOQUC010001031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1654
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98923_length_1654_cov_2.533458"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..796
                     /locus_tag="PKH07_15255"
     CDS             <1..796
                     /locus_tag="PKH07_15255"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNT36342.1"
                     /translation="REFVSDLEKIQIANNRGRLVSLMEVASLYPTTGPVSLQRDQQMR
                     LVSVLAQADRDKLEQANQAALKAIADLTPPQGSYTRLGGEFKAMREAFEQLGKALAFA
                     IFLVFLILAAQFESFTQPFIIMTTVPLSMAGVYLVLFMTGVSMSIPAYVGVIMLAGIV
                     VNNAILLVDYINVLRARGYGLVEAAVESSKTRLRPVLMTTLTTVLGMAPMALGLGSGS
                     EFYRPLALAVIGGLGLSTLFTLVLSPVLYILIAQLKTYLSGLFNRA"
     gene            complement(766..>1654)
                     /locus_tag="PKH07_15260"
     CDS             complement(766..>1654)
                     /locus_tag="PKH07_15260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013986114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNT36343.1"
                     /translation="IIAVSNAVRRSRAGLSDPRRPIGSFIFLGPTGVGKTELAKALAA
                     FLFDDEDAITRIDMTEYMERHSVSRLIGAPPGYVGYEEGGQLTERVRRRPYSVLLFDE
                     IEKAHPEVHSIFLQLLDEGRLTDGQGRTVDFRNTVVIMTSNIGGELITQEGRTQEAIS
                     RSVLDALKKTFRPELLNRISDIIVFKSLTMKEIEHIVDIQIKRVEQRLVEQRITLELT
                     EAARRLLAQEGFDPVYGARPLERTITREVENGLALKLIGGEIKPGQSVRLDVRDDKLV
                     MISEPSVCADYARLNRPER"
BASE COUNT          349 a          482 c          417 g          406 t
ORIGIN      
        1 gagggagttt gtctcagacc tggagaaaat ccagatcgcc aacaaccgag gcagacttgt
       61 ctccctcatg gaagtggcct ctctctatcc gacaaccggt cccgtgagtc ttcaacgtga
      121 tcagcagatg agactggttt ccgtcctggc tcaggcggat cgcgacaaac tcgagcaagc
      181 caaccaggca gccttgaaag caattgcaga cctgacacct ccccaaggca gttacacacg
      241 ccttggcggt gagttcaagg ccatgcgaga agccttcgag caactgggca aagcactcgc
      301 gtttgccatc tttcttgtct ttctgatttt ggcggcacag tttgagtcct tcacgcagcc
      361 ctttatcatc atgaccacgg tgcctctgtc catggcagga gtctatctgg ttctctttat
      421 gacgggagtt tccatgtcca taccagccta tgttggtgtt atcatgttgg ctggcatcgt
      481 cgtcaacaac gcgattcttc ttgtggacta catcaacgtg ctccgagcga gaggctatgg
      541 acttgtagaa gcagctgtcg agtccagtaa gacgcgactg cgtccggtct tgatgaccac
      601 cctgacaacg gtactcggta tggcaccaat ggctttgggg ctgggttccg gatcggagtt
      661 ctaccgaccc ctggcacttg ccgttatagg gggtctgggg ctttcgaccc tattcacgct
      721 ggtcttgtcg ccagtgctct atatactcat tgcacagttg aagacctatc tctccggcct
      781 gttcaaccga gcatagtcgg cacaaacaga tggctcggag atcatgacca gtttgtcgtc
      841 ccttacgtca agccgaacac tctgtcccgg cttgatctca ccgccgatca acttcagagc
      901 aagaccgttc tccacttctc tggtgatcgt tcgctccaaa ggtcgggctc cgtagaccgg
      961 atcaaaacct tcttgagcga ggagtctccg cgcggcttca gtcaactcca atgtgatcct
     1021 ttgctccacg agacgctgct cgacacgctt gatctgaatg tccacgatgt gctcgatttc
     1081 cttcatcgtc agagacttga acacgatgat gtcgctgatt cggttcagga gttcgggacg
     1141 gaatgtcttc ttaagcgcgt ccaacacact tcgcgaaatc gcttcctgcg tccgcccttc
     1201 ctgtgtgatc aactctcctc cgatgttgga agtcatgatg accaccgtgt tgcgaaagtc
     1261 aaccgttcgc ccctgtccgt cggtgagtcg tccttcgtcc aggagttgca gaaagatgct
     1321 gtgcacttcg ggatgtgcct tttcgatttc atcaaacaga agcactgaat aaggacggcg
     1381 gcggacgcgt tcagtcaact gccccccttc ttcatagccc acataacccg gaggtgcgcc
     1441 gatgagccgt gaaaccgaat gcctttccat gtattcggtc atatcaatgc gcgtgatcgc
     1501 atcctcatca tcgaagagaa acgccgcaag cgccttggcc agctcggtct tgcccactcc
     1561 cgttggtcca agaaagatga acgacccaat cggtcttcgc gggtcagaaa gccctgccct
     1621 tgagcggcgc accgcatttg aaaccgcaat gatt
//
LOCUS       DAOQUC010001032         1653 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_99019_length_1653_cov_2.734043, whole genome shotgun sequence.
ACCESSION   DAOQUC010001032 DAOQUC010000000
VERSION     DAOQUC010001032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1653
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99019_length_1653_cov_2.734043"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(110..631)
                     /gene="grpE"
                     /locus_tag="PKH07_15265"
     CDS             complement(110..631)
                     /gene="grpE"
                     /locus_tag="PKH07_15265"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HNT36344.1"
                     /translation="MDIARIFPLDETPEPVYLTDDRTVCYRVVESYSSRKSSIRRVFQ
                     TDPREEVVLGVLPILDGFERFFSLVDEQHLAENETLTNWFKAIVALYRRTVKGLQKIG
                     LEPIDTIGFSLDLERHEVVEVVPVSDQPTGTVVHEVEKGYILNGKMLRPAKVAVVKNQ
                     QEPGAAQPTGESS"
     gene            801..>1653
                     /locus_tag="PKH07_15270"
     CDS             801..>1653
                     /locus_tag="PKH07_15270"
                     /inference="COORDINATES: protein motif:HMM:NF014533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HNT36345.1"
                     /translation="MDERFFRIALAQADGVGPVTYRKILCQLRVDGKTMHELFEADAG
                     FLTAEMSLKDRVIKGILKVRESLGAIREMTEVLMEREILPLLEEDDLYPRRLCDAFGD
                     WAPPVVYCWGNCELLREASVGIVGSREPDEESLTLARRAARASVRAQRVIVSGCARGV
                     DSAAQMEALDTGGATIGVLAQGLLTRGFSGFAETISDPASCLLLSEFPPYKGWNAGSA
                     LARNRTICALSDAVLVVQAAGRGGSLECGLAALRMHKPTLTIEPLDAHAPRWSGNERL
                     INEGAFPL"
BASE COUNT          380 a          428 c          421 g          424 t
ORIGIN      
        1 gtgttcgtcg tgccaaggtc aatgcccata atccgactca ttgctgtacc tccgttatca
       61 attctccgca gatatgatga aaactaactt ataaatgcct ttcgcgctat cagctggatt
      121 ccccagtcgg ctgtgctgct cctggctcct gctggttctt tacaactgct accttcgcgg
      181 gacgaagcat cttgccatta agaatatagc ccttttcgac ctcatgtaca actgttcctg
      241 tcggttgatc cgacacagga accacttcga caacttcatg acgctccaaa tccaaactaa
      301 agcctatcgt atcaataggt tcaagaccaa tcttctgcag tcccttcacc gtccttctat
      361 acaaagcaac gatcgccttg aaccagtttg tcagtgtctc attttccgca agatgctgtt
      421 cgtccaccaa tgagaagaag cgctcaaaac catccaggat ggggagcact ccaagaacga
      481 cctcttcacg gggatcggtt tggaagactc tccgaattga actcttcctg ctgctgtatg
      541 attcgacaac tctgtaacat acagttctat catcggtcag atagacaggt tctggagtct
      601 cgtccagcgg aaaaatgcgt gcaatatcca taatgctggt gtccctcggt cctcccaatg
      661 cacctcgccg tgcaattacc gaagagagaa tacgacgtta agccaatcta ttcaagcttt
      721 tttaagcgtg ggctcagttt gccatttgaa ctcctcttgc cattgcatta ggctgtcatc
      781 aatggaggag aacccgcatt atggatgaac gctttttcag aattgctctt gcgcaggccg
      841 atggggtggg accggtcacc tatcgcaaga tactttgtca actgagggtt gatggcaaga
      901 caatgcatga gctcttcgag gcagacgcgg gttttcttac agccgagatg tccctcaagg
      961 atagagtcat caagggaatc ctcaaggtgc gtgaatctct tggcgccatt cgtgagatga
     1021 ctgaggtttt gatggaaagg gagattctac cccttcttga agaagacgat ctctatccaa
     1081 gaagactctg cgatgccttt ggagactggg cacctccagt ggtttactgc tggggaaatt
     1141 gtgagctgct ccgagaggcg tctgtcggca ttgttggtag tcgggaacct gatgaggaat
     1201 ccctaacctt ggcacgtcgt gctgcaagag cgtcggtgag ggcacaacga gtgattgtca
     1261 gtggctgtgc cagaggtgtg gactccgcag ctcagatgga agcccttgac actgggggag
     1321 caacgatagg tgttctcgcg caaggactct tgacgagggg gttctcaggc tttgccgaga
     1381 ccatctctga tccggcaagt tgtcttctgt tgtcagagtt tccaccttac aaaggctgga
     1441 atgcgggctc cgctcttgcc aggaatcgaa caatctgtgc tttgtccgat gccgtgcttg
     1501 tggttcaagc cgcgggtcgt ggtggctcac ttgagtgtgg tctcgccgcc ctgcgaatgc
     1561 acaagccaac tctgaccatc gagcctctgg atgcccatgc gccaagatgg agcggaaacg
     1621 agagactcat caacgaaggc gccttccctt tga
//
LOCUS       DAOQUC010001033         1653 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_99030_length_1653_cov_2.567584, whole genome shotgun sequence.
ACCESSION   DAOQUC010001033 DAOQUC010000000
VERSION     DAOQUC010001033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1653
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99030_length_1653_cov_2.567584"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1621)
                     /gene="selB"
                     /locus_tag="PKH07_15275"
     CDS             complement(<1..1621)
                     /gene="selB"
                     /locus_tag="PKH07_15275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HNT36346.1"
                     /translation="MERRPPGLLSHDEEAEVLLMIGTAGHVDHGKTRLVGLLTGCETD
                     RLKVEKERGLSIELGFAPCSLGGGLAAGIVDVPGHEQFIKNMVAGAAGMDMTVLVVAA
                     DDGVMPQTIEHLQIMEFLGVRLGMVVITKVDLVTPERVQEVREEIELLVDGTFLEGAP
                     VCPFSAETLEGFDLFYNTIVELATRAKIERLQGVFRMPVERVFSLAGHGTVLSGIPLA
                     GTISVGDEVQIQPSGAMARVRSMERFGHEAERGGAGQCLALNLAGLPRDGAQRGNVVT
                     RPGYVHPTSFLQIRLTTCHDLRAPLRHGEEIRFHTGTLEAHGRLGLYGCESVGKRETV
                     LASLQLNDAAGVAPTDRFVIRRHSPAITVAGGVVVSAMAEKPKMSRVKQFEHLSEKWA
                     CFGSLVSKAEFHFVESGSEGSTVAETALALLVEKSDAQQLVAQLAAKGSLTACNDGDR
                     YLHTKGMDEGREQVLRYLSQFLEKQSSSYGPTFRELTDGLGIPEPSLMVILDSLVRSG
                     AAERREGRIGLPGREKQFDGAQKDLLDEIEKT"
BASE COUNT          377 a          545 c          393 g          338 t
ORIGIN      
        1 aggtcttctc aatttcgtcg agcaaatcct tctgggcgcc atcaaactgc ttctcacgac
       61 caggcaatcc aatgcgacct tcccgtcttt ctgcagcacc cgaccgaacc agactgtcca
      121 gaatcaccat cagagatggc tcaggaatcc caagaccgtc ggtcaactcc cgaaacgtcg
      181 ggccatagga agaagactgc ttctcaagaa actgactgag atagcgaagc acctgttcgc
      241 gaccctcatc catacccttt gtgtgaagat atcgatcccc gtcattgcag gcggtcaggc
      301 taccctttgc agccagctgc gcaaccagtt gctgcgcatc cgatttctca acaaggagcg
      361 cgagagcggt ttccgcaacc gtactgcctt cagagcccga ttccacaaag tgaaactccg
      421 ccttggagac cagactgcca aaacaagccc atttctcgct gagatgttca aattgcttca
      481 ctcgcgacat ttttggcttc tcggccattg ccgatacaac gactccaccc gcgacggtga
      541 tcgcgggaga atggcggcgg atgacaaacc gatcggtggg agcaacacct gccgcatcgt
      601 tcagttgaag cgatgcgagt accgtttccc gttttcccac cgattcgcat ccgtaaagcc
      661 ccaatctgcc gtgtgcttcc aaggtacccg tgtggaacct gatctcctcg ccatgcctga
      721 gtggagcgcg cagatcatgg cacgtagtca gtcgaatctg aagaaaactc gtcggatgga
      781 catagcctgg ccgggtcacc acattcccgc gttgagcacc gtcacgaggc agtcctgcaa
      841 gattcagcgc caggcactgc cctgcaccac cgcgctctgc ctcgtgtccg aaacgctcca
      901 tgcttcgcac ccgcgccatt gcgccgctgg gctgaatctg aacctcatca ccaacactga
      961 tcgtccctgc caggggaatt ccggacagaa ccgttccatg accagcgagc gagaataccc
     1021 gctcgacagg cattcggaag actccctgca agcgctcgat ttttgcgcgt gttgcaagtt
     1081 cgacaatcgt gttgtagaag agatcgaatc cttccaatgt ctccgccgaa aaagggcaaa
     1141 ccggagcgcc ttccagaaac gttccatcca ccagcagctc gatttcctcg cgaacctcct
     1201 gcacacgctc gggtgtgacc aaatcaactt tggtgatcac aaccatgccg agtctcacgc
     1261 caagaaactc catgatctga agatgctcga tggtctgagg catcacccca tcatcagcag
     1321 cgacgaccag gactgtcatg tccatgcctg ccgcgccggc aaccatgttc ttgatgaact
     1381 gctcatgacc cgggacgtca acgattccgg ctgcaagccc accgccaagg gaacagggtg
     1441 cgaaccccag ctcgatggac aatccccgtt ccttctccac cttgagccgg tcggtctcgc
     1501 agcctgtcaa caaccccacc agccgcgtct tgccatgatc aacatggccc gcagtgccaa
     1561 tcatcaacag cacttcggct tcttcgtcat ggctcaacag tcccggggga cgacgctcca
     1621 tcagccgatc tccaactgct tcttgatttc ctc
//
LOCUS       DAOQUC010001034         1650 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_99304_length_1650_cov_3.333542, whole genome shotgun sequence.
ACCESSION   DAOQUC010001034 DAOQUC010000000
VERSION     DAOQUC010001034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1650
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99304_length_1650_cov_3.333542"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..397
                     /locus_tag="PKH07_15280"
     CDS             <1..397
                     /locus_tag="PKH07_15280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020384107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36347.1"
                     /translation="IITEGFGRIPMAHRTFNLLKSRNGERASISGRTQIRAGVMRPEV
                     IIPIPNPEGRVPRQDEIEADVSTGVKDGHQVRIIREPYFGRLGTVRTLPPELTQIETE
                     AKVRIMEITLDSGETVTVPRANVEIIEGN"
     gene            1022..>1650
                     /locus_tag="PKH07_15285"
     CDS             1022..>1650
                     /locus_tag="PKH07_15285"
                     /inference="COORDINATES: protein motif:HMM:NF022677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3034 family protein"
                     /protein_id="HNT36348.1"
                     /translation="MFRFTKRSTCADVLRIVTVWLVVLGCSSVFASPPLPKHGVEGSG
                     GIFSTYSAYLVNPAEEGKVIGLPAVSVIGVYLGNGRNLSAFTITETFWDRLELGYGLD
                     RFDTGDLNDEIERATTLRPSDDAVELHNFNLRLKLLKEGCLGEWSPTVTFGIHYKYNP
                     DIMDINRDLLGTIRSIGIKDNDGLDYTLYASKMLSLCGRPLMLNAGLRST"
BASE COUNT          376 a          437 c          453 g          384 t
ORIGIN      
        1 catcattacg gaaggcttcg gacggattcc gatggcacat cgaacattca atctcctcaa
       61 gagtcgcaac ggagaacgtg cttccatcag tggcagaaca cagatccgcg ccggtgtcat
      121 gcgtcctgag gtgatcatcc caattcccaa tccggaaggc agagttcccc gacaggatga
      181 aatcgaagcg gacgtcagca cgggcgtcaa ggacggccat caggtgcgaa tcattcgtga
      241 accttacttc ggtcggttgg gtaccgtgag aacccttccg ccagagctga cacaaatcga
      301 aacggaagcc aaagtccgca tcatggagat tactcttgat tcaggcgaga ccgtcaccgt
      361 cccgcgcgcc aatgtggaga tcatcgaagg caactgagcg gtttgcgcgg gatacctggg
      421 cggctttgtg cccgccatcc gatgacacga ggtggggtgt cttgcccgcg gggtaaaaac
      481 ggattccttg cttgacctcg gttgacaagc aacaccggat cattgattct cgctgccatg
      541 actgttcaga tctaagagac agatcctgcg atgaaaggtg gtgacggcat ggtctcaata
      601 catcgagttg ggatgagtcc ttttctgcac ggtacttgat cccaggctct gacgtcatgg
      661 cgacgtcaaa ctcttattgt caaagaacga aggggagaag atgcaatgcc gttgcgcctg
      721 agggatctgt cccgcatttt ctcgagaaac gaatcgtccg aggactccgt cttgtgattg
      781 gcgcggtttt cactttccct cagctggccg tggcataggc tctcctgcag aagacttggc
      841 atctgccctg tcgtgcgagg agcgtacttc ttctgtcgtt cagggcaaca tggttaggac
      901 acaattcctt gggagcaggg gatgaccctg ccgccgtgtg ggcaatgccc attcccgcga
      961 tgtgtctatt caaaagagaa gccataccct ctgaaggatg gtttctgcaa aggagagaaa
     1021 tatgtttcga ttcacaaagc gcagcacttg cgcagatgtt cttcgcatcg tcaccgtgtg
     1081 gctcgtcgtc ttgggttgtt ccagtgtctt cgcttcgcca ccactgccca agcatggtgt
     1141 ggaaggttct gggggaatct tctcaaccta ttcagcctac cttgttaatc cggctgaaga
     1201 aggaaaagtc attggacttc ccgccgtttc tgtcatcggg gtctatctcg gcaacggcag
     1261 gaatctcagc gcattcacca tcaccgaaac gttctgggat cgtcttgaac tcggctacgg
     1321 cctggatcgg ttcgacaccg gcgatctcaa tgatgagatc gaacgggcga cgacgcttcg
     1381 tcccagtgac gatgccgtgg aacttcacaa cttcaacctt cggctgaaac ttctcaagga
     1441 aggctgtttg ggagaatggt ctccgacagt gacgtttggg attcattaca agtacaatcc
     1501 agacatcatg gacatcaatc gcgaccttct cggaacgatt cgaagcatcg gcatcaagga
     1561 caatgatggc ttggactata cgctctatgc ttcgaagatg ctgagcctct gcggaaggcc
     1621 gctgatgctc aacgcgggac ttcggtcaac
//
LOCUS       DAOQUC010001035         1650 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_99318_length_1650_cov_2.800627, whole genome shotgun sequence.
ACCESSION   DAOQUC010001035 DAOQUC010000000
VERSION     DAOQUC010001035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1650
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99318_length_1650_cov_2.800627"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(131..700)
                     /locus_tag="PKH07_15290"
     CDS             complement(131..700)
                     /locus_tag="PKH07_15290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36349.1"
                     /translation="MLFRIIAVVAFAGAIVALPILSWLLGSKRDFTLRDILNIIVWIG
                     VLDFGEMLLYIALVLSILVLAATGFWASLVTGHEMTGLVLMTHVSAGGAFAVILALFA
                     VLQAERSRMDRLNGLSGIAMARRVSFWALLGTGLVVTLTTMLSMTPLFGTYWQQVLYE
                     VHRYGAVAAVIAALGHLHFGRFERRLESD"
     gene            complement(702..>1650)
                     /locus_tag="PKH07_15295"
     CDS             complement(702..>1650)
                     /locus_tag="PKH07_15295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36350.1"
                     /translation="GEAEDTGQPFRATMSCRGCHLGVDSEELQDAGGRFGAPHPKHPG
                     LPTLHLTRLSCTTCHSGPQPGEKAFRVQTARAHGLGLSEKHRGDKVPPFIAEPVFVRE
                     DDGTFEPHRMIWPAFWGLLTGDECKPIAIDSLEKHAGKAFGSKKTTQPEEWQPLSEDT
                     VKEALALLKSADKSGSEPVYVCGGLIYRLDAEGVLVAQEHPVAESYSWPLAHEVRPAA
                     KALGAKGCTECHSTSSSIFYSRVDIETPASLGETRSKTMLEIQGLNPTLLWLWNFSFL
                     FRPILKIVGFAASIFMGAVLLLYGLAVLQGLLKYFAREE"
BASE COUNT          438 a          501 c          405 g          306 t
ORIGIN      
        1 cttggtgcat tgcatccgca caaatccacc aacaaccaga aggacaacaa tccatgtgag
       61 agtcccgaaa accgccttaa ccttcatggc ctcagacctc cctgccctat caggggcatg
      121 tcacgccgac ctaatcactc tcgagccgcc tttcgaacct accaaaatgc agatgcccca
      181 gcgcggcaat aaccgcagcc acagcaccgt aacgatggac ttcgtaaaga acctgctgcc
      241 agtaagttcc aaacagtggc gtcatgctca acatcgtcgt caaagtcacg acaagacccg
      301 tgcctagaag cgcccagaaa gagacacgtc tcgccatggc gattccgctg agaccgttca
      361 gacgatccat cctggaacgc tctgcctgaa gaaccgcgaa cagcgcaagg atgaccgcaa
      421 aagcgccacc cgcagaaacg tgtgtcataa gaaccaaacc ggtcatctca tgtcccgtca
      481 ccagcgaagc ccagaagccc gttgcggcga gcaccaggat actaagaaca agggcaatgt
      541 agagcaacat ctccccaaaa tccaagacac cgatccagac aatgatgttc aggatatctc
      601 gaagtgtgaa atcgcgcttg gaaccgagaa gccaggacaa aatgggcagt gcaactatcg
      661 caccagcaaa cgccacaaca gcaatgatcc gaaacaacat cttattcttc tctcgcgaaa
      721 tacttcagca gtccctgaag cacggccaaa ccatacagca gaaggactgc tcccataaag
      781 atagacgctg caaaaccaac aatcttcaag atcggacgga acaggaacga gaagttccag
      841 agccacaaga gcgtgggatt gagtccctga atctccaaca tggtcttgga gcgcgtctcc
      901 ccaagcgagg cgggggtctc aatatcaaca cgcgagtaga aaatcgagga actggtggaa
      961 tgacactccg tacagccctt ggcaccgagc gccttcgccg caggtcgaac ctcatgagcc
     1021 agaggccagg aatagctttc ggcaactgga tgctcctgag ccacaagaac tccctctgca
     1081 tccagtctgt agatgagacc gccacaaaca tagaccggtt ctgaaccgga cttatcagcg
     1141 gacttgagca gtgccagtgc ctctttcacc gtgtcttcag agaggggttg ccactcttcg
     1201 ggctgtgtgg tcttcttgct gccaaacgct ttgccggcat gtttctccag agagtcaatc
     1261 gctatcggtt tgcattcatc gccggtaagc agtccccaaa acgcaggcca aatcatcctg
     1321 tgtggctcga atgtgccatc gtcttctctg acgaagaccg gctccgctat aaacggcgga
     1381 accttgtcac cacgatgctt ctcagacagt ccgagtccgt gtgcgcgagc ggtctgaact
     1441 cgaaacgcct tctccccggg ctgtggccca gagtggcagg tcgtacaact gagacgagtc
     1501 agatgcagcg taggcagacc cgggtgcttt ggatgaggag cgccgaagcg tcctcccgca
     1561 tcctgcagtt cttcggaatc cactcccaag tgacatccac gacaggacat ggttgctcga
     1621 aatggctggc cagtgtcttc ggcttcccct
//
LOCUS       DAOQUC010001036         1650 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_99334_length_1650_cov_2.477743, whole genome shotgun sequence.
ACCESSION   DAOQUC010001036 DAOQUC010000000
VERSION     DAOQUC010001036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1650
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99334_length_1650_cov_2.477743"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1428
                     /locus_tag="PKH07_15300"
     CDS             <1..1428
                     /locus_tag="PKH07_15300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36351.1"
                     /translation="PGGSNYTTFRLNMAAEPGYQGLIRRLRFDPTDRAGVSVDIDYIR
                     VIMPLVESNPAYEFDTDTAGWTAVNASVPAVAGGYLTTTGTSNDPRLISPDLTLNSKT
                     DQFAYVAMRAFAGTAGSIIFQNPLVAPGFIGANEKVFAIPTNADRALYNINLNSLANF
                     QPGTITKLRFDPSNAAGDIMVDKINVLSRKQAYGASWEFNIPGYADGWSVPMGMTAVA
                     NGTDISGLTTSTTPRLLSPMNVYINGDKETQLLLRMRVSKGTRAQLFFMREQDTVFTA
                     SQWLTIPIANNTDYVLYNITMNQIPSYSGLIKRLRLDPTNAKNAQVDIDYIRAIGPAP
                     EGTPEWRFETETDTEGWSPLGTMPALSVASGVLSATSAGSTSSMRTANTLNVDASTKK
                     IVGIRMRVGGLGTTAVFNWRRSGDVGFDITRRKVFNIINNGNFNTYYLDLSSDPNYTG
                     TVTQIRMVPTDAVGLFEIDWIGFID"
BASE COUNT          395 a          436 c          434 g          385 t
ORIGIN      
        1 cccggtggaa gcaactatac gaccttcagg ttgaacatgg ctgctgagcc tggatatcaa
       61 gggctgatcc gacggcttcg ctttgatccg accgatcgag cgggggtcag tgttgatatt
      121 gactacatca gagtgatcat gcctctggtt gagagcaacc ctgcctatga gttcgatact
      181 gacactgccg gctggactgc cgtcaatgcc agtgtacctg ctgtggctgg tggttatttg
      241 accacgaccg gcacgagcaa tgacccgagg ttgatctcgc cggatctgac gctcaactcc
      301 aagaccgacc agtttgccta tgtcgccatg agagcttttg ccgggactgc tggaagcatc
      361 atcttccaga atccacttgt ggctcctggc ttcatcgggg caaatgagaa ggttttcgcc
      421 attccgacaa atgcggatcg ggcgctctac aacatcaact tgaactcgtt ggcaaacttc
      481 cagcctggaa caatcaccaa actgcgattc gatccgagca atgcagcggg tgatatcatg
      541 gtggacaaga tcaacgtcct gtccagaaag caggcctatg gagcatcctg ggagttcaat
      601 atcccgggtt acgcggatgg ctggagtgtc ccaatgggta tgactgccgt ggcaaacggt
      661 acagatatct ccgggctcac cacatccaca acgccaaggc tcttgtcccc gatgaatgtg
      721 tatatcaatg gagataagga aactcagtta ctcctgagaa tgcgtgtttc gaagggcacc
      781 agagcacagt tgttcttcat gcgagagcag gatactgtgt ttacagcttc acagtggttg
      841 acgattccga tcgccaacaa caccgactat gtcctttaca acatcactat gaaccagatt
      901 ccttcttact ccggactgat caagcgcctg agacttgatc cgaccaacgc caagaatgcc
      961 caagttgata ttgactacat tcgggctatt ggtccagcgc cagaaggaac tcccgagtgg
     1021 cggtttgaga ccgagactga cacagaagga tggagtccat tgggaaccat gcctgccctg
     1081 agcgtcgctt caggggtcct gtccgccacc tcggcgggtt caaccagcag tatgcgaact
     1141 gccaacacac tgaatgttga cgcttcaacg aagaagatcg tcggaatcag gatgcgtgtt
     1201 ggtggtcttg gaacaacagc tgtgttcaac tggagacgta gtggtgatgt cggttttgac
     1261 attactcgcc gtaaggtctt caacattatc aacaatggaa acttcaatac ttactatctc
     1321 gacctttcga gtgatccaaa ctacactgga accgtgactc agatccgcat ggttccaacc
     1381 gatgcggtag ggctctttga gatcgactgg atcggtttca tagactgagc cactcggtac
     1441 gacacgtgac cttcgggtgg ggagcaggct ttcctgctcc ccatctgttt tgggggtatg
     1501 ccttgttgct cttgcgcccc gagccgacta tagtatccgc aacttgagag aggagtctgc
     1561 cacgggatgc aaaccgcaag ggagatcccg ctttgttcat acacaacact ccgcatcgga
     1621 ggaccagcag agcatcttgc cacagcgaat
//
LOCUS       DAOQUC010001037         1649 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_99404_length_1649_cov_3.161857, whole genome shotgun sequence.
ACCESSION   DAOQUC010001037 DAOQUC010000000
VERSION     DAOQUC010001037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1649
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99404_length_1649_cov_3.161857"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /locus_tag="PKH07_15305"
     CDS             complement(<1..205)
                     /locus_tag="PKH07_15305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36352.1"
                     /translation="MTVVSGSTHTRRRALSSAWWQTSVHLALILGLCACLPRLVPGAT
                     VSRIRRLDCWDDARKTTRVTVQVD"
     gene            complement(202..753)
                     /locus_tag="PKH07_15310"
     CDS             complement(202..753)
                     /locus_tag="PKH07_15310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36353.1"
                     /translation="MDEKKKKKKEVIEDETQQQETEATEPTTLIEKEIVRFNEIVAKD
                     PQRAYSRYGYSLLCSLPPEQMAREVLRLRKRTDDPFDLYNRGVFEAQDGKPEKALKDF
                     QKSYAVLAEMDEATAVHYLRRQPTFLADLAFNLYLCHCEQDQKSEGKKEFKQVQKLAK
                     GLYPDQETSSALWEAFAEVESEL"
     gene            complement(805..>1649)
                     /locus_tag="PKH07_15315"
     CDS             complement(805..>1649)
                     /locus_tag="PKH07_15315"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNT36354.1"
                     /translation="LLPLEVTGALLRDQEFTITGLLLSFRDISESREIDKLRRLDQMK
                     SNFVSVASHELRTPLTSMIASLSLLNSSGDAGMNESQRSLIRILYRNSKRLLDLVNDI
                     LDLSRLESETITLRSKKTRIGSLLLHAIDSMRQAAEAKGIQIQCKASESLEFVLDEEK
                     FEQVLLNLLSNAIKFTPEGGRIALSAERAPGSELRIQVSDTGTGIPDEDTERIFDKFY
                     QVEDAPNRTSQGTGLGLAICRKIVELHQGTIWVESELGKGSTFVIEVPCPATVESPAS
                     VSTE"
BASE COUNT          380 a          470 c          394 g          405 t
ORIGIN      
        1 aatccacctg aaccgtcacg cgcgttgtct ttctggcatc atcccagcag tccagccgcc
       61 gaatccttga aacggtcgct ccgggaacca aacgcggcag acaggcacat aatcccagaa
      121 tcaaagccaa gtgaacagag gtctgccacc aggcggaact cagggctcgc cgtctcgtat
      181 gagtggatcc cgaaacaact gtcataactc actctccact tccgcaaagg cctcccagag
      241 agcagaggac gtctcttgat ccggatagag accttttgcc agtttctgaa cctgcttgaa
      301 ttccttcttg ccttcgcttt tctgatcctg ctcacaatgg cagagataca ggttgaaagc
      361 cagatctgcc aggaaggtcg gctgtctgcg cagatagtgc acagccgtcg cctcgtccat
      421 ttcagccagg acggcatacg atttctggaa gtccttcagc gccttttccg gtttgccatc
      481 ctgcgcctca aaaacgcccc gattgtacaa atcgaacgga tcgtctgttc gtttgcgcaa
      541 gcgcaatacc tctcgtgcca tctgttccgg aggcaaactg cacaacagag aatacccgta
      601 gcggctgtag gctctttgag gatccttggc cacaatctca ttgaatcgaa cgatttcctt
      661 ctcgatgagt gttgtcggtt cggttgcctc ggtctcctgc tgctgcgtct cgtcctcgat
      721 cacttctttt ttcttcttct tcttctcgtc cactgattcc tccacaattc caaattcgtt
      781 tactgtcgga aacggaatct ttcctcactc tgtggacacg gacgccgggg actccaccgt
      841 ggccggacaa gggacttcga tgacgaaggt tgagcctttg ccaagttcac tctccaccca
      901 aatcgttccc tggtgcagtt cgacaatctt tcggcagatg gccaaaccca gtcccgttcc
      961 ttgagacgtc ctgttcggtg catcctcgac ctgatagaac ttgtcgaaaa tgcgttccgt
     1021 gtcctcgtcg ggtatcccgg ttcccgtatc ggaaacctga atccgaagtt cggatcccgg
     1081 cgctctctcg gcagagagtg cgattctgcc tccttcaggc gtaaacttaa tagcattgga
     1141 cagcaaattc agcaagacct gttcaaactt ttcctcatcc agcacaaatt ccagtgattc
     1201 agaggctttg cactgaatct gaatcccctt ggcctccgca gcctggcgca tcgagtcaat
     1261 cgcgtggagc agcagggagc caattcgagt cttcttggaa cgcagggtga tggtttccga
     1321 ctcaagacga gacaagtcga ggatgtcgtt gaccaagtcg agaagtctct tggagttgcg
     1381 gtagagaatg cgaatcaaag aacgttggct ctcgttcatg ccggcgtccc cactactgtt
     1441 caagagcgac aacgaagcga tcatggacgt cagcggagtt ctcagttcat gggaagcgac
     1501 cgagacaaag ttggatttca tctgatcaag acgcctaagt ttgtcaattt ccctcgactc
     1561 agagatatct cgaaaactca acaacaaacc ggtaatagtg aattcctgat ctcgaagcaa
     1621 agctccggtg acttcaaggg gtaataaag
//
LOCUS       DAOQUC010001038         1648 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_99483_length_1648_cov_3.820465, whole genome shotgun sequence.
ACCESSION   DAOQUC010001038 DAOQUC010000000
VERSION     DAOQUC010001038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1648
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99483_length_1648_cov_3.820465"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..541)
                     /locus_tag="PKH07_15320"
     CDS             complement(<1..541)
                     /locus_tag="PKH07_15320"
                     /inference="COORDINATES: protein motif:HMM:NF012795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HNT36355.1"
                     /translation="MCQTLSDIGESGFLNKLAEWLPGAGDGIVVPAGDDAAVVQWASG
                     QGVITTDTLVEGTHFRLEWSAPEDIAIKLLHMNLSDIAAMGGVPDFGVLALTACGSTQ
                     LDWLERFYHALLKSCDTTNVRLVGGDTTEGSSIILTLTVVGHVTGSAVTFATAQPGDA
                     VFVTGDLGSAQAGLEVLLRK"
     gene            complement(548..>1648)
                     /locus_tag="PKH07_15325"
     CDS             complement(548..>1648)
                     /locus_tag="PKH07_15325"
                     /inference="COORDINATES: protein motif:HMM:NF012361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HNT36356.1"
                     /translation="VVPVPEDQLPVLLPEIEDYKPDGSGKSPLHRVETFVKTTCPKCG
                     GAAERETDVSDNFLDSAWYFLRYPSTQCNDKPYDTEINRKWLPVDMYIGGNEHAVLHL
                     MYTRFICMALHDMGLLEFEEPFKKFRAHGLIIKDGAKMSKSKGNVINPDAYLADYGAD
                     TFRTYLMFLAPLYEGGDFRDEGIQGVRRFLERLFRFATETIFDDTGTEPVETTRKLHK
                     TIKKVTEDIQSLNYNTAIAALMELLNLFREKQCRRKSEFEQLLLLVASFAPHLAEELW
                     ERIGNKPSIYDHQLPKHDESLVVDDVIELVVQINGKLRSRLQVPRDLPEAEAIERAKK
                     DANVAKYLEGMQIVKVVYVPNRLANIVCRPGK"
BASE COUNT          386 a          467 c          409 g          386 t
ORIGIN      
        1 cttttctgag aagcacctcc aagccagcct gcgccgaacc gagatcgcct gtcacgaaaa
       61 cggcatcacc cggttgtgca gtcgcaaacg tcacggcgga acctgtcaca tgccccacta
      121 cggtcagtgt cagtatgata gaggaacctt ccgttgtgtc acccccaacc aaccggacgt
      181 tggttgtgtc acaggatttc aaaagcgcat ggtagaagcg ttcgagccag tccaactgag
      241 tgctgccaca ggccgtcagc gccagaactc cgaagtcggg aacaccgccc atcgcggcga
      301 tgtcactgag gttcatgtga aggagtttga tcgcaatatc ttctggagcg ctccactcca
      361 accggaagtg agttccttcc accaaggtgt cagttgtgat gacgccttgt cctgaagccc
      421 actgcaccac ggcagcatca tcacctgccg gaacgacaat tccatctccg gctccaggga
      481 gccattcagc caacttgttc agaaagccag attcaccaat gtccgagaga gtctgacaca
      541 ccaatcatca cttccctggt cggcacacga tattcgccag acggttggga acgtagacca
      601 ctttgacgat ctgcattccc tcaagatact ttgcaacgtt cgcatccttc ttggcgcgct
      661 ctattgcttc ggcttccgga agatctctgg gcacttgaag tcgagaacga agtttcccat
      721 tgatctgaac cacgagttcg atgacgtcat ccacgaccag actctcgtcg tgctttggca
      781 gctgatgatc gtagatgctc ggcttgtttc caatgcgttc ccagagttct tcggccagat
      841 gaggggcaaa cgaagcgacc agcaaaagca gctgttcaaa ctcggacttg cggcgacact
      901 gcttctctcg aaacaggttc aacagttcca tcaacgccgc gattgccgtg ttgtagttca
      961 gtgactgaat atcctcggtg accttcttga tcgtcttgtg aagttttcga gtggtctcaa
     1021 ccggttcagt gccggtgtca tcaaaaattg tttcagtggc aaatcggaaa agccgctcta
     1081 gaaatcgccg aactccctga atgccttcat cgcgaaagtc cccgccctca tagagaggag
     1141 cgaggaacat caggtacgtt cgaaatgtat cggcaccata atccgccaga tacgcatcgg
     1201 ggttgatgac gttccctttt gacttgctca tcttggcacc gtctttgatg atcaagccgt
     1261 gagcacggaa cttcttgaac ggttcttcga actccagaag tcccatatcg tgtagtgcca
     1321 tacagatgaa gcgagtgtac atcaggtgca gcacggcatg ttcattgccg ccaatgtaca
     1381 tatccaccgg caaccacttt cggtttatct cggtatcgta gggcttgtcg ttacactggg
     1441 ttgatgggta gcgcaggaag taccaggccg aatcaagaaa gttatctgaa acatcggtct
     1501 ctcgctccgc cgctccgcca cactttgggc atgtcgtctt gacgaacgtt tcgacacgat
     1561 gaagaggaga cttacccgaa ccatccggct tgtagtcttc gatttcagga agcagtaccg
     1621 gcagctgatc ttcgggcaca ggcaccac
//
LOCUS       DAOQUC010001039         1648 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_99527_length_1648_cov_2.525424, whole genome shotgun sequence.
ACCESSION   DAOQUC010001039 DAOQUC010000000
VERSION     DAOQUC010001039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1648
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99527_length_1648_cov_2.525424"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..785)
                     /locus_tag="PKH07_15330"
     CDS             complement(<1..785)
                     /locus_tag="PKH07_15330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36357.1"
                     /translation="MNRNQRAHRDPLRVLLPIFLIATVGALAGLVFFMHQGLKSLQEE
                     AHLQMKELRSASIQISTDALQELGEAIIRQRAEDIAREVTLYLSLHPEMTVADLQKDP
                     IFQNIAVQPVGKTGYSALTDYDTLTCRFHKSPKIVDVDLSSLSEKLPGFWKIMHATKG
                     GNPVGGYYDWLEPDGSIKQKYMFIAIAQGRTAEGVGFSVASTTYIDEFSEPAVLLQDQ
                     ATKSIDKAVARLETSSAEMQSTTLGMMTGIVITTAIVFVCMLYL"
     gene            complement(830..>1648)
                     /locus_tag="PKH07_15335"
     CDS             complement(830..>1648)
                     /locus_tag="PKH07_15335"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNT36358.1"
                     /translation="DGSTAWQMLQTPGMPSLIVLDWMMPGMDGLDICRRIRKRGTQPP
                     EYVILLTSMDRKQDIVAGLESGANDYITKPFDNDELRARLQVGQRVLELQTELVAAKK
                     AFEFQATHDALTGLLNRRAILERLEREMSRCRRQESELSIMLLDIDHFKDVNDNYGHA
                     VGDEVLREFSQRLRTVLRDYDSIGRYGGEEFLIVLPDICAIRNEETFERIRYSIGSEA
                     FRTTAGALSVTTSAGATCSDGKSTVDGLIHEADSALYRAKNAGRNRVVFAERHP"
BASE COUNT          371 a          461 c          423 g          393 t
ORIGIN      
        1 agatagagca tacagacgaa gacgatggcc gtggttatga cgatgcccgt catcatgcca
       61 agtgtggtgc tctgcatttc cgcgcttgac gtctcaagac gcgcaaccgc cttgtctatg
      121 gatttggtgg cttgatcctg aagaagcacc gcaggttcag agaactcatc aatgtaggta
      181 gtggacgcga cactgaaacc cacaccttcg gcggttctgc cttgagcgat ggcaataaac
      241 atgtatttct gtttaatgga gccgtccggt tccaaccagt catagtaacc acctaccggg
      301 tttcctccct tggtggcgtg cataatcttc cagaagccag gcagcttctc gctcagactg
      361 gataaatcta cgtcaacaat cttcggactt ttgtgaaagc ggcaggtcag tgtgtcgtag
      421 tcggtgagcg cggagtatcc agtctttccc accggttgta ccgcaatgtt ctgaaagatt
      481 ggatctttct gcaggtcggc aaccgtcatc tcaggatgca ggctcagata gagcgtgacc
      541 tcacgggcga tatcctcggc acgctgacgg atgatggcct cacccaattc ctgaagcgca
      601 tcggtgctga tctgaatcga agccgagcgc agttccttca tctgaagatg ggcttcttct
      661 tgcaaagatt tgagaccttg gtgcatgaag aaaaccaggc ctgccagcgc cccaactgtg
      721 gcaatcagaa agataggcaa aagaacccga agtggatctc gatgtgctcg ttgatttctg
      781 ttcattgttt ttgctccgat ccccttgccc ccatgttgat gccttccctc tacggatgcc
      841 gctcagcgaa gaccactcga tttcttccgg cgttcttggc acggtacaac gcggaatctg
      901 cctcatgaat cagtccgtcc accgttgact ttccatcgga acaagtcgca cccgcgctgg
      961 tcgtaacaga gagtgctccc gcggttgttc taaaggcctc actgccgatg gaataccgaa
     1021 ttcgctcaaa cgtctcttcg ttgcgtatgg cacagatatc tggaagcaca atgaggaact
     1081 cctcaccgcc gtagcgacca atggagtcat agtctcgcag caccgtccga aggcgctggg
     1141 agaactctcg caacacttca tctccaacgg catggccgta gttgtcattg acgtctttga
     1201 aatgatcgat gtccagtagc atgatgctga gttctgactc ctggcgtcgg caacgcgaca
     1261 tttcgcgctc aagacgctcc aatatggcac gccggttgag aaggccggtc aacgcatcat
     1321 gcgtggcctg gaactcgaat gccttcttgg ccgccacaag ctcggtctga agctccaaga
     1381 ctcgttgacc tacctgcaaa cgagcgcgca actcatcatt gtcaaagggc ttggtgatgt
     1441 agtcgttggc accagattcc agtccagcca caatatcttg cttgcgatcc atggacgtga
     1501 ggagaatcac gtactcggga ggctgtgttc cacgctttcg gatccgccga cagatatcca
     1561 acccgtccat gccgggcatc atccagtcca gaacgatcag acttggcatt ccaggtgtct
     1621 gaagcatctg ccaagcggtg cttccatc
//
LOCUS       DAOQUC010001040         1647 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_99582_length_1647_cov_3.515704, whole genome shotgun sequence.
ACCESSION   DAOQUC010001040 DAOQUC010000000
VERSION     DAOQUC010001040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1647)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1647)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1647
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99582_length_1647_cov_3.515704"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1098
                     /locus_tag="PKH07_15340"
     CDS             <1..1098
                     /locus_tag="PKH07_15340"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HNT36359.1"
                     /translation="GARTARHPRAAGWLAKVGSVAHFDHFPEPQDYAQVKTYSERTLT
                     LSWDKMIEACGQIVDCLKAYDPNMWIESSVSCHESESLLVTSGGVCHHSTSTGWGLGA
                     SVQRIQGTDMLLTGYGRGWCDLNEFFDPDVVSQKVLENLRWAEKTCAPPRGRVPVFVP
                     SERMGMWLWPVLMGVNGRSVAKGESPLASRLGQQILACNLTLRDDPHRDYAHGACEID
                     PDGIPTRKHTIVEKGVLKMFLYDLDTAGLAGTSPTGSPGCGTCRPCLDPGKDSQIKLL
                     SQVEDGLYVCDLIGFGQSNIINGDFSCNVGLGYRIKNGEIVGRVKNTMLAGNIYDLLS
                     GGVRLSRETDYTGAWPCGVIEGMACSSSETN"
     gene            1111..>1647
                     /locus_tag="PKH07_15345"
     CDS             1111..>1647
                     /locus_tag="PKH07_15345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36360.1"
                     /translation="MERTWKVLLFGNSDQENRIFSERLRNLSNCRCCVDVVSSVDEAS
                     SRIAAADYDVYMVDYQIGAEWIASLQANEHQRATLFLIEEDFDPQVLAEDLRTHVFYI
                     FKNRVGLSPVDAWVQGRERPARVESLKDALLFRSISRATDISRDFEMLRETEERYRKL
                     VENAQDGIAIVLDQKILFV"
BASE COUNT          360 a          412 c          483 g          392 t
ORIGIN      
        1 ggcgcccgta cggcgcgcca tccgcgtgcc gcgggctggc tggccaaggt gggcagtgtg
       61 gctcatttcg atcacttccc agagccccag gactacgctc aggttaagac ctattcagag
      121 aggacattga ctttgtcctg ggacaagatg atcgaagcct gtgggcagat cgtggactgt
      181 ctcaaggcct acgatccaaa tatgtggatc gagagttctg tttcatgcca tgaatccgag
      241 tcgctcctgg ttacttccgg aggtgtctgt catcattcca catcaaccgg atggggcttg
      301 ggggcgagtg ttcaacgcat ccagggcacc gacatgttgt tgacaggtta cggaagagga
      361 tggtgcgatt tgaacgagtt cttcgaccct gatgttgtct cacagaaggt gttggagaat
      421 ctccgctggg cggaaaagac atgcgcccca ccgcgcggga gagttccagt tttcgtgcct
      481 tctgaacgga tgggaatgtg gctttggcct gttctgatgg gcgtcaatgg ccgcagtgtt
      541 gccaaggggg aatccccgtt ggcctccaga cttgggcagc agattctcgc ctgcaatctc
      601 acactgcgag atgatccgca ccgcgactac gcacatggcg catgcgagat tgatcctgac
      661 ggcattccca cgcgcaaaca tacgatcgtt gagaagggcg tcctcaagat gtttctgtac
      721 gatctggata ccgcgggact ggcgggcact tcaccgacag gaagtcccgg ttgcggcact
      781 tgtcgtccct gtctcgatcc ggggaaggac tcacagatca aattgctttc tcaggtcgaa
      841 gatgggctgt acgtatgtga cctgattggc tttggccaga gtaacatcat caatggggat
      901 ttctcctgca acgtcgggct ggggtatcgc atcaagaacg gagaaatcgt tggccgtgtt
      961 aaaaacacca tgcttgcggg aaacatctac gacttgctct cgggcggtgt acgactgagt
     1021 cgtgagaccg actacacggg ggcttggcct tgtggagtta tcgaaggtat ggcctgcagc
     1081 agttccgaaa ccaactgacg agaagcgctt gtggaaagaa cctggaaagt cctgcttttc
     1141 ggcaactccg atcaggagaa ccgaatattc tctgagagac tcagaaatct ctcgaactgc
     1201 cgatgttgtg ttgatgttgt gtcctccgtt gacgaggcat caagtcggat cgcggcggcg
     1261 gactatgacg tttatatggt ggactaccag attggggctg aatggatcgc ttctcttcag
     1321 gccaacgagc accagcgcgc aacgcttttt ctcatcgaag aggattttga tcctcaggta
     1381 cttgcagagg atctgcgaac tcatgttttc tacatcttca agaatcgcgt cggactaagt
     1441 cccgttgatg cctgggttca gggacgtgag cgtcctgcca gagtggagtc cctcaaagac
     1501 gctcttctat tccgaagcat ctccagagca acggacatct cacgggattt tgaaatgctt
     1561 cgtgagactg aggagcgata ccgaaaactt gtcgagaacg cgcaggatgg catcgccatt
     1621 gtgttggatc agaagattct cttcgtg
//
LOCUS       DAOQUC010001041         1643 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_100067_length_1643_cov_2.799748, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001041 DAOQUC010000000
VERSION     DAOQUC010001041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1643)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1643)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1643
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100067_length_1643_cov_2.799748"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..531
                     /locus_tag="PKH07_15350"
     CDS             <1..531
                     /locus_tag="PKH07_15350"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNT36361.1"
                     /translation="QLEVAAYAGLAIEALERVCHVRPQASLVLVGGGRYLAELQKMVD
                     RKGLNEKVLLTGYRPSCEVREFLANADVGLAPFEETPVTRAKSPLKVVEYLAAGLPIV
                     GSHVGEVPTMIGNAGRTVVPDDPDAFAQAILDVLASPERWQGYSREARRRVQEVYNWS
                     HTTDEILRAYEKAIEG"
     gene            535..>1643
                     /locus_tag="PKH07_15355"
     CDS             535..>1643
                     /locus_tag="PKH07_15355"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNT36362.1"
                     /translation="MAAPTILMLNHNLVRRGTFFRCIQFARCLVKSGFDVTLISASDQ
                     PRLRTRRSFDHSADATHQALTIIETPRFGSVGQHDGGWAPVDIVFRLGHVLRHRYDIV
                     HAFDHRPNVSFPWFLSRCAHKSIHVADWADWWCRGGIITSRRRFQFLDGWEAALEEGI
                     KRAADGVTVTSRVLEQRALSLGILPEKILYLPSGADTERIKPLDRTECRKRLNIPLGA
                     RVVSFVGHALWDLDFLVNAFRIVSKADPKAMLYLVGSEWKEMKGVLSAGYSGLHIRSA
                     GLVSPEQLPMHLAVADVHALPLQDTLVNQARGPIKLGDYMASGRPIVTQGVGDTGALV
                     EKEAIGLVTGTTAEDFARGMATLLADPELCVQLGHR"
BASE COUNT          337 a          412 c          500 g          394 t
ORIGIN      
        1 cagttggaag tggcggccta tgccggtttg gcgatagaag cccttgagcg ggtctgtcat
       61 gttcgtccac aggcatccct ggttctggtg ggtggaggaa gatatctcgc cgagctgcag
      121 aaaatggtgg atcgaaaagg gctgaatgag aaagtccttc tcaccggata cagaccgtct
      181 tgtgaagttc gggagttcct ggcaaatgcc gacgttggtc tggcaccttt tgaggaaacg
      241 cctgttacac gcgccaagag tccgctcaag gtcgttgaat acctggctgc gggacttccc
      301 attgtcggca gccatgttgg ggaagttccg acgatgattg ggaacgcggg gcgaaccgtt
      361 gtacctgatg atccagacgc ctttgcccaa gcgattctcg acgtgctggc gtctccagag
      421 cgatggcagg ggtattcccg tgaagcacgc aggagagtgc aagaagtgta caactggtct
      481 catacgacgg atgagattct gcgtgcttat gagaaagcca ttgaagggtg aaccatggca
      541 gcgccgacca ttctgatgct caatcataac ctggttcgca gggggacttt tttccgatgt
      601 attcagtttg ctcggtgtct ggtgaagagt ggcttcgacg ttacgcttat ctctgcgtct
      661 gaccaaccac gattgcgtac tcgacggagt ttcgaccaca gcgccgatgc gacgcatcag
      721 gcgctgacga tcatcgagac gccgcggttt ggatccgttg gacaacatga cggtggatgg
      781 gctcccgtgg acatcgtgtt tcgattggga catgtgttgc gacaccgcta cgacattgtt
      841 cacgccttcg accaccgacc gaatgtatcg ttcccctggt ttctttcgag gtgtgcacac
      901 aaatcgatcc atgttgccga ctgggcagat tggtggtgcc gaggtggcat catcacctct
      961 cggcgaaggt tccagtttct ggacggatgg gaagcggcct tggaagaagg gatcaaacgt
     1021 gctgcggatg gtgtcacggt gaccagccgt gtgcttgaac agcgtgcctt gtctttgggt
     1081 attttgccgg agaagatact ttaccttcct tccggcgcgg ataccgagcg aatcaagcct
     1141 ctcgatagga cagaatgcag gaagagactg aacattcctt tgggtgccag ggttgtctcc
     1201 tttgttggtc acgcactgtg ggatctggat tttctggtga atgcctttcg aatcgtatca
     1261 aaggccgatc ccaaggccat gctttatctg gtcggaagcg agtggaagga gatgaaaggg
     1321 gtcttgagtg cgggctattc cggcctgcat attcgctctg caggtctcgt ttcacctgaa
     1381 cagcttccta tgcacctcgc cgttgcggac gttcatgcac tcccgcttca ggacactctg
     1441 gtcaaccagg cgcgtggtcc cataaaactc ggcgactaca tggcttcagg ccgtcccatc
     1501 gtcacgcaag gggttggcga cacaggtgcg ctggtcgaga aagaagcgat tggcctggtc
     1561 accggaacaa ccgctgagga ttttgccaga ggtatggcaa cgctgttggc cgatccagaa
     1621 ctctgcgtgc agttgggtca tcg
//
LOCUS       DAOQUC010001042         1643 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_100072_length_1643_cov_2.687657, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001042 DAOQUC010000000
VERSION     DAOQUC010001042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1643)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1643)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1643
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100072_length_1643_cov_2.687657"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..541
                     /locus_tag="PKH07_15360"
     CDS             <1..541
                     /locus_tag="PKH07_15360"
                     /inference="COORDINATES: protein motif:HMM:NF020130.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HNT36363.1"
                     /translation="LDWNDRGTCVLFGDGAGATVLRADSTPGVLKVNISADGTYGDLL
                     QILGGGSRNPCSQKVIDEGLQYVRIKGREVYKTAVRVMSQSVEEILKQLNMTGDDLAL
                     FIPHQANLRIIEAVAAKLNYPMEKTLVNIDRYGNTTAATIPLAIDEVVRNGRLKSGDT
                     MGMVAFGGGFTWGAAVIKY"
     gene            642..1565
                     /gene="fabD"
                     /locus_tag="PKH07_15365"
     CDS             642..1565
                     /gene="fabD"
                     /locus_tag="PKH07_15365"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HNT36364.1"
                     /translation="MKTAFIFSGQGTQKVGMGKDLAEKYPLAKSLFERADAALERNLS
                     ALCCNGPEEVLSRTENAQPAIFTLEVACLELLREQGITPDAVAGHSVGEYAALYAAGA
                     FDFETGIQILHERGMLMREAAENNAGGMMAVMGLPLEKALAIAKEFPPDLCADIAAYN
                     SPQQLVISGKVEDLQKAGEIAKAKGAKRVIPLKVAGAFHSRLQAEAREKMKAFCQTLT
                     LKPLSLPYVANYSGKLESDPVVIRNMLCEQLTSPVQWLGCLESLKAQGIGTYIECGGA
                     VLTAFVTAFDKEAVVRNVFDAQTLESVQGEA"
     gene            1570..>1643
                     /locus_tag="PKH07_15370"
     CDS             1570..>1643
                     /locus_tag="PKH07_15370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018249677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP reductase"
                     /protein_id="HNT36365.1"
                     /translation="MSLEGKVALVTGSARGIGKAIALRL"
BASE COUNT          394 a          412 c          466 g          371 t
ORIGIN      
        1 tcttgactgg aacgaccggg gcacctgtgt tttgttcggt gacggtgctg gtgccacagt
       61 cctgcgtgcc gattcaacac caggtgtgct caaagtgaac atctctgcag acggcaccta
      121 cggagatctg cttcagattt tgggtggtgg ttccagaaat ccctgcagcc agaaggtcat
      181 tgacgaagga ctgcagtacg ttcggatcaa aggccgggaa gtctacaaga ccgcagttcg
      241 ggtcatgtct cagtctgttg aggaaattct gaaacaactc aacatgacgg gggacgattt
      301 ggctttgttc attccacacc aggcaaacct gagaattatc gaagccgttg ccgccaaact
      361 caactacccg atggaaaaga ccctggtaaa cattgaccgc tacggcaaca cgacggctgc
      421 caccattccg ttggccattg acgaagtagt gcgcaacgga cggcttaagt ctggcgacac
      481 gatgggaatg gtcgctttcg gcggaggatt tacctggggt gctgccgtaa tcaaatacta
      541 aagaggtttt tctttgcatt tcgagacgct tttcatgtat gtaagcgtct tcaaaaaggt
      601 aaacgaacaa cacagattct tcgactatgg gaattgagag tatgaaaaca gcctttatct
      661 tttccggaca agggactcaa aaagtgggta tgggcaaaga ccttgccgaa aagtaccccc
      721 ttgccaagtc ccttttcgag cgagccgatg ccgctttgga aagaaacctc tcggcacttt
      781 gctgcaatgg cccggaagag gttttgtcca gaaccgaaaa cgctcagccc gcgattttca
      841 cccttgaagt cgcctgtctg gaacttctgc gcgaacaagg catcacgcct gacgcagtcg
      901 ccggacacag cgtcggagaa tatgctgctt tgtacgccgc cggagcgttc gattttgaga
      961 ccggcatcca aatcctgcat gagcgcggga tgctcatgcg cgaggcggct gagaacaatg
     1021 cgggtggtat gatggccgtc atgggtctgc ctctggagaa agcccttgcc atcgccaagg
     1081 agttccctcc cgatctctgc gcggatatcg ccgcgtacaa ttccccgcag cagttggtga
     1141 tcagcggcaa ggtggaggat ctccagaaag cgggtgagat agccaaagcc aaaggcgcta
     1201 agcgtgtcat cccactgaag gtcgcaggag cttttcactc ccgacttcag gcagaggcgc
     1261 gggagaagat gaaggcattt tgtcagacac tgactctcaa gcctctgtct ctcccctacg
     1321 tagccaatta cagtgggaaa ctggagtccg atccggtcgt cattcgtaat atgctctgcg
     1381 agcagctgac ttcaccggtg cagtggctgg ggtgtttgga atcactcaag gcacaaggaa
     1441 tcgggactta tatcgaatgc ggtggcgccg tgctgaccgc ctttgtgact gcctttgaca
     1501 aagaggctgt ggtcagaaat gttttcgatg cacagacgct cgagtctgtt caaggggagg
     1561 cataggataa tgtctttgga aggaaaagtg gctctggtaa caggatcggc tcgtggaatt
     1621 ggaaaggcga tcgccctgcg act
//
LOCUS       DAOQUC010001043         1641 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_100309_length_1641_cov_2.338588, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001043 DAOQUC010000000
VERSION     DAOQUC010001043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1641)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1641)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1641
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100309_length_1641_cov_2.338588"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..382)
                     /locus_tag="PKH07_15375"
     CDS             complement(<1..382)
                     /locus_tag="PKH07_15375"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNT36366.1"
                     /translation="MSYRVRPGDTLTQIGQRYGTTAEVIAAINGLDKPDRLQANQRIL
                     VPKRSFGLSRLWTKRGQFVVVKSGDTVIGLSTAHGVDALEVIRCNRIQDPKRLAVGQV
                     LFLPGARPGKVVAEPAPKPKAPKPK"
     gene            complement(944..>1641)
                     /locus_tag="PKH07_15380"
     CDS             complement(944..>1641)
                     /locus_tag="PKH07_15380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36367.1"
                     /translation="LWVCPLECWSVQKPAARPKQAKPAVKSAPAPKLKQAVQTDDEQF
                     LEQIKGRQTSVEEQLGVSAKDSRAQVVMRQNRAQMQLNMSEDQQQQFKRITDGFDTLY
                     GSSNVTEEQRTQLCDEVKKTLCGFQEPSEQALDQFCLNLVATVIQGELSPNEATQIYQ
                     NVRFYVETQNVTQEHAEWVVNDSRHIANSSNCTPDHALKTVSCVQRVIETAWKAKKEA
                     EKAAAEVEDATAQ"
BASE COUNT          368 a          437 c          416 g          420 t
ORIGIN      
        1 gcttcggctt gggtgccttc ggcttcggtg ctggttcggc cacaaccttt ccaggtcgag
       61 cgccaggcaa aaagagaacc tgtcccactg ccaagcgctt cggatcctga attctgttgc
      121 agcggatgac ttcgagtgca tccacgccat gggctgtgga cagcccgatg acagtatctc
      181 cggatttgac aaccacaaac tgaccgcgtt tggtccagag acgactgaga ccaaaactcc
      241 tcttggggac aaggattctc tgatttgcct gaagtctgtc cggtttgtca agaccgttta
      301 ttgctgcaat gacttccgcc gtggtaccat accgttgacc tatctgtgtc agcgtgtcac
      361 ccggtctgac gcggtaagac acaaaatcat atccctgagg cggttgggtt cgaagaggtg
      421 cccccagatg aacacatccg cccgaaaaag acaaaaagac agcacaaaac accggcaatc
      481 cgaaggatgt caggcttctc gaaaacctat acttttgtgc tatccaaatc tttactacct
      541 caaggtttat caacctgaag ttaaagttta tcttaatata tcccatccac taactcgatg
      601 caagtttttt ctgttcgcga agcgtatcgg ctccgttgca cagggtatgc ttttcatttt
      661 gaacccttcg gcggattcta tcctgggcaa agccgaagga ctcaggataa gtcctgcgac
      721 ggtctgagag aaaagaggaa tccctctctt cttcagaatc cacagagagt tctctcgtcg
      781 agcgtcaaaa tgacaaggtg aggagcctcg ggatcccagt cgggcaagcc gaaacaagcg
      841 aagacgtctc gttgggaaac caagagatgc cagatgaaca agcgcgtgac ttggacatcc
      901 tggaagagtt gtgagcacgt cttcaggcaa ggagaaggag cgcctactgg gctgtcgcgt
      961 cctcaacctc ggcagcagct ttttccgctt ctttcttggc tttccaagcc gtttcgatga
     1021 cccgctgcac gcaagatacg gtcttcaagg cgtggtcggg ggtacagttc gacgaattgg
     1081 cgatgtgcct ggagtcgttc accacccatt cggcatgttc ttgggtgacg ttctgggttt
     1141 ccacgtagaa gcgaacgttc tggtatatct gcgtggcttc gttgggcgat agttcccctt
     1201 gaatgactgt ggcgaccaga ttcaggcaga attggtcaag cgcttgttca ctcggttctt
     1261 gaaagccgca gagagtcttc ttcacctcat cacacaactg cgttctttgc tcttctgtaa
     1321 cattcgaaga tccatagaga gtgtcaaaac catccgtgat cctcttgaat tgctgttgtt
     1381 gatcttcgga catgttcagt tgcatctggg cacggttctg tctcatcaca acttgggctc
     1441 gcgagtcctt tgcactgaca ccaagttgtt cctccaccga agtctgacgc ccctttatct
     1501 gttccagaaa ttgctcatcg tctgtctgta ctgcctgctt cagttttggt gcgggagcac
     1561 tcttgactgc tggttttgcc tgcttgggac gagcagccgg cttctgaaca gaccagcact
     1621 ccagcgggca cacccagagc a
//
LOCUS       DAOQUC010001044         1640 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_100396_length_1640_cov_2.473186, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001044 DAOQUC010000000
VERSION     DAOQUC010001044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1640
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100396_length_1640_cov_2.473186"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1640
                     /locus_tag="PKH07_15385"
     CDS             <1..>1640
                     /locus_tag="PKH07_15385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36368.1"
                     /translation="GREAQEAYQASRYSVLSRDYFARFTKDPDAKPNSDEVALEADWR
                     IILSDKAHPLSETIARDLADFLYQRMELTLPIARASTEELTAGVEKAISLIDSGSGEE
                     SEAFSIAVHRNRVIICASSAEGTRNGVVRLVDTIGFRQAPLLTLGEQRYEPRLGVRLG
                     VVPWMGSLRDVVFMGYNAISAGGASLYALSTSDAIPELKERRRPDSLAAIQKSVAAAQ
                     KYGLKTYVLLETRQRFPKDDPIFTAYPEIRGTPTWAADSEYVLCTEHPLVQRFLMESV
                     EGLFRAAPGLDGVLFINGGEGFYHCYMRSFGAAKGRSSCPRCDAVGVDTVVANLSNNM
                     AAAARRMNPKAEVLAWPYSAEHVWSSDRAQAGFIARLKPGAGVFTEIEKDEYVDKPEG
                     VRKHLWDYSIDLIGPGERAKQQVIACKAVGAPIYMKSEPELAFEAPRLSHIPCMDRWA
                     DRAESLASCGADGAFVFPAFRPCYGNVTAELYKHFWWTPTPTKDEVLTNLARRVAGPE
                     AGTHLRNAWKFVSEAVEWSPELPSYYTGPYYLGPAHPM"
BASE COUNT          374 a          421 c          484 g          361 t
ORIGIN      
        1 taggaagaga agcccaggaa gcctaccagg cgagtcgcta ttcagtcctt tcaagagact
       61 atttcgcccg ctttaccaaa gaccctgacg ccaagccaaa ttcggatgaa gttgccttgg
      121 aggcagactg gcggatcatc ctttctgaca aggcacatcc gctctcagag accattgcca
      181 gggatctggc cgattttctc taccaacgga tggagttgac ccttccaata gcaagggcat
      241 ctaccgagga actcacagct ggcgttgaga aggctatttc tctcatagac tccggtagcg
      301 gcgaagagtc tgaggccttc tcgatagccg tacaccgcaa cagagtaata atttgcgcca
      361 gcagtgccga gggaacccga aacggagtcg tgagactggt agacactata ggtttccggc
      421 aagctccgtt gctgaccttg ggtgaacaga gatacgagcc acgcctgggt gttcgtttgg
      481 gtgttgttcc gtggatgggt tctctgcgtg atgtcgtctt tatgggatac aacgcgattt
      541 ctgcgggggg cgcttcgctg tacgcactgt ccacttcgga cgccattccg gagctgaaag
      601 aaaggcgaag gccagacagt ctggcagcca ttcagaagtc cgttgcagca gcacagaagt
      661 atgggctcaa aacatacgtt ttgctcgaaa cccggcagag attcccgaaa gacgacccga
      721 tattcaccgc ctacccggag attcggggaa caccaacctg ggctgcagac agcgagtacg
      781 ttctctgcac cgagcatccg cttgtacagc ggtttctgat ggaaagcgtg gagggactct
      841 ttcgcgccgc acccggcctc gacggagtct tgttcataaa cggtggagag ggtttttacc
      901 actgctatat gcgctcgttc ggtgctgcca aaggacgttc gagttgtcct cgttgtgatg
      961 ctgttggggt ggacacggtc gtggcgaatc tctccaacaa tatggcggct gccgcgcgca
     1021 ggatgaaccc gaaggcagag gtactcgcat ggccttactc tgcagagcat gtatggtcgt
     1081 ctgaccgcgc tcaggcaggg ttcatcgcga ggctgaaacc cggtgcagga gtttttacag
     1141 aaattgagaa ggacgagtac gttgataagc ccgaaggtgt ccgaaaacac ctctgggact
     1201 acagcataga tttgataggg cctggtgagc gggcgaagca gcaggtcatt gcatgcaagg
     1261 cagtcggtgc ccctatctac atgaagagcg aaccggaatt ggcattcgaa gcgccgaggc
     1321 tttctcacat cccatgtatg gaccgttggg cagatcgagc ggaatctctg gcatcatgtg
     1381 gagcagatgg cgcattcgta ttccctgcat tccgaccatg ctatggcaat gtgacagcgg
     1441 aactgtacaa gcatttttgg tggacgccga caccaacgaa ggatgaagtg ttgacaaacc
     1501 ttgcccggcg tgttgctggt ccagaggcgg gaacgcatct tcggaatgcc tggaagtttg
     1561 tgtccgaggc ggtggagtgg tcaccggagt tgccttccta ctataccgga ccttattacc
     1621 tgggaccggc acatccgatg
//
LOCUS       DAOQUC010001045         1638 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_100553_length_1638_cov_4.237524, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001045 DAOQUC010000000
VERSION     DAOQUC010001045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1638
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100553_length_1638_cov_4.237524"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..372)
                     /locus_tag="PKH07_15390"
     CDS             complement(<1..372)
                     /locus_tag="PKH07_15390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNT36369.1"
                     /translation="MRLVLIKRILYLGMVSLWRHRLRSALTVLGIVFGVCSVIAMLAI
                     GEGASYEAQEQIKRLGSQNIIIKSVKPPSKETVNVETSFLREYGLTYKDAERIRDTIP
                     AVEVIVPSRNIRANVVYRGKML"
     gene            complement(471..1100)
                     /locus_tag="PKH07_15395"
     CDS             complement(471..1100)
                     /locus_tag="PKH07_15395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013360675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNT36370.1"
                     /translation="MGTVPVNALDQVSVSFEAGEYAAIMGPSGSGKSTFLNLLGCLDT
                     PSEGTYLLDGLDVSNLSDSELSDIRRIKIGFIFQSFNLINELDVLENIQVPLFYQAVD
                     EQESHDRAVMLSQAVGLGHRLHHRPAELSGGERQRVAIARSLANDPVLILADEPTGNL
                     DSKSGREILDILHRLNAEGKTILVVTHDENVGREANRIVRFKDGTIVTS"
     gene            complement(1111..>1638)
                     /locus_tag="PKH07_15400"
     CDS             complement(1111..>1638)
                     /locus_tag="PKH07_15400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36371.1"
                     /translation="LNPNLKVFDVDVMLGVTPPELKPGMSAEVRIIVDELEDVVYMPI
                     QAVASKGEKRVCFVMTALGPKEREVQVGEYNDKFIEIKSGVDVGERVVLNAANLLEEG
                     AERFKTRKAKEVDRAAIEQGTPIAEIQNQEQEPAVSSTEVQEEPTVRPPAPEVEKPSE
                     GEREPRASRRGRQNQ"
BASE COUNT          386 a          464 c          377 g          411 t
ORIGIN      
        1 cagcatttta ccacgataca ccacattggc gcggatattc cgtgagggaa caatgacctc
       61 aaccgcagga atggtgtctc gaatgcgttc ggcgtcttta tatgtcagcc catattcccg
      121 gagaaaactg gtctcgacat tgactgtttc cttgctggga ggtttcacac tcttgatgat
      181 gatgttctgg cttcccagtc gcttgatctg ttcctgggct tcatagctgg caccctcgcc
      241 aatagccaac atcgcgatga cggagcatac cccaaacacg atacccagaa ctgtcaacgc
      301 agaacggagc cgatgtcgcc acaggctgac catacccagg tagagaattc tttttatcaa
      361 aactaatcgc atgagactct ttctcgcctg atcaactgct gggtcacatc ccagatgtca
      421 gttcgatatc tccggttcca gaaacaaaat cctatctcgc agatcgcggc tcacgaagtc
      481 acaatcgtgc catctttgaa gcgcacgatc ctgtttgcct ccctgccaac attctcatcg
      541 tgcgtcacca cgagaatcgt ttttccttcc gcattcagcc gatgcaggat atccagtatt
      601 tcacgtcccg atttggagtc tagatttccc gtaggctcat cggcaagaat cagcaccggg
      661 tcgttggcaa gagaacgtgc gatggctaca cgctggcgtt cacctcccga gagttctgca
      721 ggccggtgat gcagtctgtg tccaaggcca acggcctgtg agagcatgac ggctctatca
      781 tgactctctt gctcatcaac agcctgatag aacagaggta cctggatgtt ctccaggacg
      841 tccagttcgt tgatgagatt gaaggattgg aagataaaac cgatcttaat gcgccgaata
      901 tccgatagct cggaatcgga caggttggag acatccagac catccaaaag ataggttcct
      961 tcggaaggcg tgtccagaca gcccaacaga ttcagaaaag ttgactttcc tgaacccgaa
     1021 ggtcccataa tggcagcata ctctcccgct tcaaaggaaa cgctcacctg atcaagagca
     1081 ttcacaggaa cagtccccat gatatactgc ttactgattc tgacgacctc gacgagaggc
     1141 acggggttct ctttctcctt ccgatggttt ttcgacttca ggtgcaggcg gacgaacggt
     1201 cggttcttcc tgcacctctg tgctgctcac agcaggttcc tgctcttgat tttgaatctc
     1261 agcgatggga gttccttgct cgatagcagc ccgatccact tctttggcct ttcgagtctt
     1321 aaaacgctct gctccctctt ccaaaaggtt cgctgcattc aggacaacgc gttctccgac
     1381 atccacgcca ctcttgatct caatgaattt gtcgttgtac tcgcccacct gaacttcgcg
     1441 ttccttgggg ccgagggcag tcatgacaaa acaaactctc ttctcaccct ttgaggcaac
     1501 cgcctggatg ggcatgtaga caacgtcctc gagttcatca acgatgatac gcacctcagc
     1561 cgacattccc ggcttcaact cgggtggtgt gacaccgagc attacgtcca cgtcaaaaac
     1621 ttttagattt ggattcag
//
LOCUS       DAOQUC010001046         1637 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_100685_length_1637_cov_3.232617, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001046 DAOQUC010000000
VERSION     DAOQUC010001046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1637
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100685_length_1637_cov_3.232617"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..942
                     /locus_tag="PKH07_15405"
     CDS             <1..942
                     /locus_tag="PKH07_15405"
                     /inference="COORDINATES: protein motif:HMM:NF037126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36372.1"
                     /translation="NSRTRGAVCARARTWGYWKPSGRETIDMKPTFSERMGHKPATTV
                     LQTDHMGDSLRNSIWNVVASSVKEILRPDRHNGYDTIEYGWERLAEDMTQHFLKRPLD
                     DVPHRGQCRGWVRDLLYAFSWNEVYDWIEFINWLAVKLKVRYLPKGIDGQFNAVLEEE
                     MSAYRIIQGHVVPIGSAIEMRCIQDASASSLQSGCSGAYHHIERALLCFSKKPVPDYY
                     NTVKESISAVESVACQVTGNEKAQLGPALKILEGRGRLHSAMRAAFEKLYGYTSNEGG
                     IRHAMLEDSGAVGFDEAKFMLVACSAFVHFLISKTAQ"
     gene            1041..1331
                     /locus_tag="PKH07_15410"
     CDS             1041..1331
                     /locus_tag="PKH07_15410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006669980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HNT36373.1"
                     /translation="MGVDELLREKRSEILRIAALHGGENVRVFGSVVRGQAGPDSDID
                     ILVRLEEGRTLIDHVALIQDLEELLGRKVDVITEGGLRGRFRDLVLSEAVPL"
     gene            1328..>1637
                     /locus_tag="PKH07_15415"
     CDS             1328..>1637
                     /locus_tag="PKH07_15415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018396077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HNT36374.1"
                     /translation="MSRDLLYLEHMRDALRRILELVGPDHDLFLNDRTKQEAVIRNFE
                     VLGEAAKRISAATRQIEPDVPWRQFAAFRDVLIHKYDAIDLRVVWETAVRRVPDALHK
                     I"
BASE COUNT          382 a          395 c          479 g          381 t
ORIGIN      
        1 aattctcgaa ctcgcggcgc ggtatgtgcg cgtgcgcgga cttggggata ctggaagcct
       61 tctggcagag agaccattga tatgaaacca accttctctg agcgcatggg gcataagcct
      121 gcaacgactg ttcttcaaac cgaccatatg ggggactctt tacgcaatag catctggaat
      181 gttgttgcct catccgttaa ggagatactc agacctgaca ggcacaatgg ctatgacact
      241 attgaatatg gctgggagcg tcttgcagag gatatgaccc agcattttct taagaggccc
      301 ctcgacgatg tgccgcatcg tggccagtgc agaggatggg ttcgggatct tctttacgcc
      361 ttctcgtgga atgaagttta cgattggatc gagttcatta attggttggc agtgaagctc
      421 aaggtgcgct acttaccaaa gggaattgat gggcagttca acgccgttct cgaggaggag
      481 atgtcagcat atcggatcat tcaaggtcat gttgttccca taggaagcgc aatcgaaatg
      541 cggtgcatac aagatgcttc cgcgtcctcg ctgcagagcg gctgttctgg ggcttatcat
      601 cacattgaac gtgcgcttct gtgcttttcc aagaaacctg ttcctgatta ctacaacacc
      661 gtgaaagaat caatcagcgc tgtagaatct gtcgcgtgtc aagtcacggg aaacgagaag
      721 gcgcaacttg gtccagcgtt gaagattctc gaaggccggg gtaggttaca ctctgctatg
      781 agagcagcct tcgaaaaact ctatggatat acgagcaatg aggggggtat ccggcacgca
      841 atgcttgagg acagcggagc agtcgggttt gatgaagcaa agttcatgct tgtcgcgtgt
      901 tcggctttcg tgcacttcct gatcagcaag accgcccaat agcgtcatcg aatcctggaa
      961 aaaggggcca ctgccccttg cttgcgttgg tactgttcac gaacaagaca tgctagaatc
     1021 aggggcagga ggccaaactc atgggcgtcg acgaactctt gcgcgagaag cggtctgaga
     1081 tactccgtat cgccgctctg catggcggtg agaacgtgcg ggttttcgga tcggtagtgc
     1141 gtggacaggc cggtccggac agtgatatcg acattctggt tcgcctcgag gaagggagaa
     1201 cgcttatcga ccatgtggcg ctgatccagg atctcgagga attattggga cgcaaagtgg
     1261 acgtaatcac cgagggcggg ctgcgcggcc ggtttcgtga tcttgtactt tccgaggcag
     1321 ttccgctatg agtcgggatt tgctctatct tgagcacatg cgcgacgccc tacggcgtat
     1381 ccttgagctt gtcgggccag accatgatct gttcctgaac gatcgcacga aacaggaggc
     1441 cgtgataagg aactttgaag ttctcggcga agccgcaaag cggatttcgg cagcaacgcg
     1501 gcagatagag ccagatgttc cctggagaca attcgccgca tttcgcgacg tgctcattca
     1561 caagtatgac gccatcgacc tgcgagtcgt ctgggaaacc gcggtaaggc gggttcctga
     1621 tgccctgcac aaaatag
//
LOCUS       DAOQUC010001047         1637 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_100699_length_1637_cov_2.845133, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001047 DAOQUC010000000
VERSION     DAOQUC010001047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1637
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100699_length_1637_cov_2.845133"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..730
                     /locus_tag="PKH07_15420"
     CDS             <1..730
                     /locus_tag="PKH07_15420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36375.1"
                     /translation="MDTIESASACMPIGLKVTLTDGRVHRLLVSASACQQIQSDEHLS
                     TDCFAVVSESDSGTDKILMAVGGTEVQLSDRRIQVPETLVGRVTAVDYQDRKVTVSFP
                     KSRPRAETLIGRYVRVFSDQHSCMRRISSVRKGLSKYEIGFEDCDLLIGRVKVTEFDP
                     SGQSFTSQTGLPWGDQLLGTTVVTETRERLGRLQAREDTVFHLDTAAKVKDIQDSDGT
                     GAVELYFGEIGVGDRVEIEGAMEE"
     gene            840..>1637
                     /locus_tag="PKH07_15425"
     CDS             840..>1637
                     /locus_tag="PKH07_15425"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNT36376.1"
                     /translation="MSKSLRYGIIGCGVIGPWHARSVALVDGAELVACCDVIPEKVEK
                     FAAEYGGQTYTDYKKMLAEAKLDIVSICTPSGLHAEMAICAAENGVNVLSEKPLDISD
                     PAMTRMIKTCREKGVKLACIFQRRTSPLWMKVREIVQTGKLGKMVLGDAYLKYYRSPE
                     YYASADWRGTWKLDGGGALMNQGVHCVDLVQWIMGPVKSVTAYAAPLVRKIEVEDTSV
                     AILKYASGAFGVIQGTTSVCPGMNHRLEFHGEFGTLRVEGEKIVELRS"
BASE COUNT          385 a          392 c          492 g          368 t
ORIGIN      
        1 gatggacacc attgagtctg cttcagcgtg tatgcctatt ggcttgaaag tgaccctgac
       61 tgacggcaga gtccacaggc tcctcgtctc agcaagtgcc tgtcagcaga ttcaatcaga
      121 tgaacatctc agcacggatt gctttgctgt agtgtccgaa tccgattcgg gcacggacaa
      181 aatccttatg gcagttggtg gcaccgaggt tcaactgagt gacaggagga ttcaagtacc
      241 cgaaacgctt gtaggccgag tcactgccgt tgactatcag gatcgtaagg tcaccgtgtc
      301 gttccccaag tcgcgcccca gagcagagac tctgattggc cgatatgtgc gcgtcttcag
      361 cgatcaacat tcctgtatgc gccgaatctc ctcagtccga aaaggcttga gcaagtatga
      421 aatcggattc gaggattgcg atcttctgat cggtcgagtg aaggtcaccg aattcgatcc
      481 gtctggccag tctttcactt ctcagacagg gcttccttgg ggagatcagc tgcttggaac
      541 gaccgtcgtc actgagacga gagagcgtct gggaagactt caggcccgtg aagacacggt
      601 gtttcatctg gatactgccg ccaaagtgaa ggacattcag gactccgatg gaaccggagc
      661 agtcgagctc tactttggag agataggtgt cggagacagg gtggagatcg aaggggcgat
      721 ggaagaatga tgcttggtga gaatatgacg ggtgatgggc ttgcgaagca gtccgacccc
      781 tgctatatag aagaaccatt gaaagccgtg ttcattcaaa atatctatcg gaggaagaaa
      841 tgagcaagtc tctgcggtac ggaatcatcg gttgtggtgt gattggtccc tggcatgcca
      901 ggagcgtggc attggtggac ggtgcggaac tggtcgcctg ctgtgacgtc attccggaaa
      961 aggttgagaa gtttgcagcc gaatacggcg gacagactta caccgactac aagaagatgt
     1021 tggcagaagc caagctggac atcgtctcca tctgcacccc cagtggactt cacgccgaga
     1081 tggccatctg cgccgcggaa aatggggtca acgtcctttc cgaaaagccg ctcgatatct
     1141 ccgatccagc catgactcgg atgatcaaga cctgtcgcga gaagggtgtt aagctggcgt
     1201 gtatcttcca gcggcggacc tctccgctgt ggatgaaggt tcgtgagatt gtccagacag
     1261 ggaagctggg caagatggtt ctgggcgatg cctatctgaa atactacaga agccctgaat
     1321 actatgccag cgcagactgg cgtggcacgt ggaaactgga tggtggtggg gcgttgatga
     1381 accagggtgt gcattgcgta gatctcgtcc agtggatcat gggtcccgtc aaatcggtca
     1441 cagcctacgc cgctccgctg gttcgaaaaa tcgaagtgga agacacgtcc gtcgcaattc
     1501 tcaagtatgc cagtggcgct ttcggcgtca ttcagggaac aacttcggtt tgtccgggca
     1561 tgaatcaccg cctggaattc cacggtgagt ttggaaccct gcgcgtagaa ggcgagaaga
     1621 ttgtcgagtt gcgtagt
//
LOCUS       DAOQUC010001048         1636 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_100791_length_1636_cov_3.669829, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001048 DAOQUC010000000
VERSION     DAOQUC010001048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100791_length_1636_cov_3.669829"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..460
                     /locus_tag="PKH07_15430"
     CDS             <1..460
                     /locus_tag="PKH07_15430"
                     /inference="COORDINATES: protein motif:HMM:NF015148.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="OPT/YSL family transporter"
                     /protein_id="HNT36377.1"
                     /translation="SQFIWSIDTVPSEMFPYANQFWELRAYQSGLLYSATLPGETASP
                     FRDAFVPRYLLSGFGLALIVYALLSHFGLPIFLVYGVVRGLDQSVPHAIIPMFLGALL
                     GRFGCRKWFGEDWPKYRIVFAAGFTAGIGLITMLSLGIVFMSKSVVKLPV"
     gene            465..1445
                     /locus_tag="PKH07_15435"
     CDS             465..1445
                     /locus_tag="PKH07_15435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36378.1"
                     /translation="MTHQGYLPLAWCGWQMRIPLDWRPLRVSGNRLKGMMTVGDSTQA
                     HMQIKWLQAKSRGFVFAEWVSRRVATLQRKDKTETELCRLAGTDEPCRIVQSLPIRET
                     SRRCAYAYSREASQVIEVVSFTPFQTRHSCSPKRSQTSVSESSSETLPIQGGAHPLFS
                     LEFSDLHSPTRWAVFDVSFITPTGYDIETHRINLGDMAFRFAHRGGQRLVLRQVYPSE
                     TALARREMSKWLDDRPFKEHKLLKCDQIPQTITFDSTGTQGLLRRGRKRLPFPLHSVA
                     TRHSISAIAHDTDFSRLLMAEYDSPSSADEEVVLQVMRDMNWHKAKGAGR"
BASE COUNT          372 a          462 c          405 g          397 t
ORIGIN      
        1 tagtcaattc atctggtcta ttgatacggt gcccagcgag atgttcccct acgccaacca
       61 attctgggaa ctgcgtgcct accagtcggg gcttctctac tccgccacac ttcctggtga
      121 gacggcgagt ccgttccgcg acgcctttgt tcctagatat ctcctcagcg ggttcggatt
      181 ggctctgatc gtgtatgcac ttctgagcca ctttggactg cctatcttcc ttgtgtacgg
      241 tgtcgttcga ggactggatc aatccgtccc gcatgcgatc atcccaatgt tcctcggcgc
      301 tctgctcgga cgatttggat gccgaaaatg gtttggagaa gactggccta aataccgaat
      361 tgtcttcgcc gcaggattca ccgcgggcat cggactgatc acgatgctca gccttggcat
      421 cgtcttcatg agcaagagtg ttgtcaaact gccggtgtaa catcatgacc catcaaggat
      481 atctcccctt ggcttggtgc ggttggcaga tgcgcatccc gctcgattgg cgtcccctaa
      541 gggtctctgg caaccgactc aaaggcatga tgaccgttgg tgactcaact caggcccaca
      601 tgcagatcaa atggcttcaa gccaagtccc gaggttttgt ctttgcagaa tgggtcagcc
      661 gacgagtagc cacgctacag cgcaaagaca agacggaaac agaattgtgt cgccttgcgg
      721 ggactgatga accctgtcgg atcgttcaaa gtcttccgat tcgcgagaca tctcgaaggt
      781 gcgcctacgc ctactcacgc gaagcctctc aggtgattga agtcgtttca tttacaccct
      841 ttcagactcg acattcctgc agtcccaaga gatcgcagac atcggtctct gaaagcagtt
      901 cagaaacctt acccattcag gggggagcgc atccattgtt ctctcttgaa ttttcggatc
      961 ttcattcacc cacacgttgg gctgtgtttg atgtcagttt cataacgcca acaggctatg
     1021 acattgagac tcaccgaatc aacctcgggg acatggcatt tcgttttgcc cacagaggtg
     1081 gtcagagatt ggtgcttcga caagtctatc cgtccgaaac agcgcttgcg cgtcgtgaga
     1141 tgtccaaatg gctcgacgac cgtcccttta aggaacacaa acttctcaag tgcgaccaga
     1201 ttccacagac catcaccttc gattctactg gaacgcaggg cttgctccgc agaggtcgca
     1261 agagacttcc ctttccgctg cattccgttg caactcgaca ctccatctcg gccatcgctc
     1321 atgacacaga tttcagccgc ttgctgatgg cggaatatga ctccccttcc tctgcggatg
     1381 aagaagttgt tctgcaagtc atgagagata tgaactggca caaagcgaag ggagccgggc
     1441 gataatgtca ccgccaaagc caaatcaaaa acggcttccc aagaatcttt cgcttgcctg
     1501 tcgtcccaga cggctaccta tcgtcaggca gcaagagaca gacgatggaa agctccgtgt
     1561 cacggttcac ataaagccat accgatgggt acagttcttt accggtcgcc cagaagccga
     1621 gaggacattt gcactt
//
LOCUS       DAOQUC010001049         1630 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_101497_length_1630_cov_2.408889, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001049 DAOQUC010000000
VERSION     DAOQUC010001049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101497_length_1630_cov_2.408889"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            137..790
                     /locus_tag="PKH07_15440"
     CDS             137..790
                     /locus_tag="PKH07_15440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36379.1"
                     /translation="MGTTAPALEALSILRMSIQEGKRIASEDIRSPESIAALWQLFYY
                     LFHDRWDVEAGVSILERLSGQCAEYSKWFNDPTMARVELLARLAYGYEVLGKTKRAEL
                     NWRRAFKELVGEETSAGGDLSEIRETVRVAVVEYEMEEPSEQKNVSQQTRDDGNAEVA
                     MSASASEGRKPSGCGCFTGCAESVTAGGACGSSGETANVISGSKPSCGCFGTKKPTE"
     gene            1253..>1630
                     /locus_tag="PKH07_15445"
     CDS             1253..>1630
                     /locus_tag="PKH07_15445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HNT36380.1"
                     /translation="MSTQVQQCCACQNEVSEEDILKRLDEVIESYRNRPGALIPVLQI
                     AQTLFGYLPESALKRISLGLNKPYSEVAGVVGFYSFFSTSPRGKHLIRVCLGTACYVR
                     GGRQVLDALERRLGVTVGETTADR"
BASE COUNT          381 a          342 c          496 g          411 t
ORIGIN      
        1 tcaagacgcc gaacaaagta gcagaggagg tgtcgaatat ctctctgccg gtaggaatgc
       61 agtgtgcatc gtcgggatat ctccatcaat gaacgagctc agtcgtatta gagatctttg
      121 gtcacgcaga gatgcgatgg gaacaaccgc ccctgccttg gaagcactct ccatactaag
      181 gatgtccata caagaaggga agaggatagc ctcggaagat atacgttctc ccgagagcat
      241 cgctgcactt tggcaactgt tctactatct cttccacgac agatgggatg ttgaggcagg
      301 tgtttccatt cttgagagat tgtctggaca gtgtgccgag tattctaaat ggttcaatga
      361 tcccacaatg gctcgggtcg agttgctggc gcgtctggcg tacggatacg aagtcctcgg
      421 taagacgaag cgagccgaac tcaattggag aagagctttc aaggaattgg tgggagaaga
      481 gacttctgcc ggcggagatt tatcggagat aagggagact gtccgagttg cggttgttga
      541 atacgagatg gaagaaccct ctgagcagaa gaacgtctca caacagacga gggatgacgg
      601 gaacgcagag gtcgcgatga gtgcatctgc ttctgaggga aggaagccat caggatgcgg
      661 atgttttacg ggatgcgcag agagcgtgac agcaggaggg gcttgtgggt cttcgggtga
      721 gaccgcaaat gtgatcagcg ggtccaaacc ctcctgcggc tgttttggca cgaagaagcc
      781 gacggagtag agagacggaa gtagttaagc gtgtattgtg tgcaggggca ggcgtaacgg
      841 tctgtctctg cacacaccgt cgttcggctg cggaagcaga acgcgggcgg ttcatcaggg
      901 tgttcgtatg cttcgggtgc agcgctgtct tctgtaagag tctacgcttc ggcgtactgt
      961 tctctaagaa caactgatgg gtctggcgat ccatccttgc gcagttcccg ttttgtgtct
     1021 gagttcacaa ttccatgagt acggtttggg gagttttgag acagtgcttg agaagtcaac
     1081 aagaatcgct gctaagtgtt tgtctttcaa agtgttacat cgttaatttt ttatcgcttg
     1141 attcgaatgc ttcacttctc tagactccat ccgtttatta tcttgtatgc taacatggag
     1201 aaccgtataa acgagacggt gtgttgagag tccgtatttg agagaggtca gtatgagtac
     1261 gcaagtgcag cagtgttgtg cgtgtcagaa tgaagtctct gaggaggata ttctcaagag
     1321 actcgatgag gtaatcgaga gttatcgcaa cagaccaggc gctctgattc cggtgcttca
     1381 gattgcccag acactttttg ggtaccttcc tgaaagtgct ttgaagcgaa tcagcctggg
     1441 cttgaacaaa ccctacagcg aagtggctgg cgtggtgggt ttctattctt tcttctccac
     1501 aagtcctcgt ggcaagcatc tgattcgggt ttgtttgggc acagcctgct acgttcgtgg
     1561 aggcaggcag gtgttggatg ctttggagcg aaggctgggg gtgactgttg gcgagaccac
     1621 ggcggatcgg
//
LOCUS       DAOQUC010001050         1629 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_101553_length_1629_cov_3.082592, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001050 DAOQUC010000000
VERSION     DAOQUC010001050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1629)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1629)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1629
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101553_length_1629_cov_3.082592"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1117)
                     /locus_tag="PKH07_15450"
     CDS             complement(<1..1117)
                     /locus_tag="PKH07_15450"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNT36381.1"
                     /translation="MTEPLAVALNTVSKHYTLGHEANPTLKEMVLRLGFRKRWRTPFK
                     ALDSVTVEIPWGQSVGIVGRNGSGKSTLLKLVAGITAPSSGTVSVSGKVLPLLELGAG
                     FHPELTGRENIFLTGAVLGLSERVVRERLDEIVAFAELKSFIDVPVKHYSSGMFLRLG
                     FSVGIQMDPDILVLDEVMAVGDHRFREKSAEEILRMQQMGKTVLFVSHDMASVRMLCQ
                     RVIWLEKGRVVFDGSPAEGIVQYRQALARQEHAEQEGKSEQLISLSMIRPGSTEAVDV
                     FEVGEPLEIIPLFDPSLSGMKGTLVVEIQDDQGTVLHRTQGIYQVRSTDQAQSNPVKV
                     RSLNLLPGLYALHVIFKQDEVEKPIEELATFSVGPCSR"
     gene            complement(1147..>1629)
                     /locus_tag="PKH07_15455"
     CDS             complement(1147..>1629)
                     /locus_tag="PKH07_15455"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HNT36382.1"
                     /translation="ALGIRLSSKGTILFKQERVGKGGKIFVLYKFRTMYMDVPNYEEA
                     PTRSDDPRVFGFGRFLRRSSLDEIPQLINVLKGDMSMVGPRPEMPFIVEKYDVWQRRR
                     LLVKPGITGLWQIIGRKDLPLHANLEYDLYYVQNMSLFNDILILLKTIPVVFFGKGAY
                     "
BASE COUNT          401 a          463 c          404 g          361 t
ORIGIN      
        1 ttcgagagca cggaccaaca gagaaggtcg ccaattcctc aatgggtttc tcgacttcat
       61 cctgtttgaa aataacatgc agcgcataca gcccgggcaa tagattcaac gagcgaacct
      121 tgacaggatt tgactgtgcc tgatcggttg agcgaacctg atagattccc tgtgttcggt
      181 gcagcacagt tccctggtcg tcctgaatct cgactaccag tgttcccttc attcccgata
      241 gagatggatc gaaaagtggg atgatctcca gcggctcgcc cacttcaaat acatctaccg
      301 cttcagtcga accaggtcga atcatcgaaa gagagatcag ttgttcggac tttccttcct
      361 gctctgcgtg ttcctgtctg gcaagcgcct gccgatactg cacaatgccc tccgccggtg
      421 aaccgtcaaa aacgactcga cctttctcca gccatatcac cctttggcac aacattcgaa
      481 cagaggccat gtcatgggag acgaagagaa ccgtcttgcc catctgctgc atcctcaaaa
      541 tctcctcggc gctcttttcg cggaagcgat ggtctcccac cgccatgact tcatccaaca
      601 cgagaatatc cggatccatc tgaatcccca cggagaagcc cagccgaaga aacatgccac
      661 tggagtaatg ctttacggga acatcaatga aggatttgag ttcagcaaaa gccactatct
      721 cgtccaaacg ttcgcgaacc acgcgttcgg aaagacccag aacagctcct gtgaggaaga
      781 tattctcgcg accggtcaac tccggatgga aaccggcacc cagttcaagc aacggcagga
      841 ctttccccga aacagaaact gtgcctgacg agggtgcagt gatgccagca accagtttca
      901 ggagtgtgct cttgcctgag ccgtttcttc cgacgatccc aaccgactga ccccagggga
      961 tttccacagt gaccgagtcc aacgctttga atggtgtacg ccaccgcttg cgaaacccca
     1021 gtcggagcac catttccttg agtgtggggt tggcttcatg gccgagcgtg taatgcttgg
     1081 agacagtgtt taacgcaacg gcaagcggct cggtcacagg cttgtctcct cggcgcgttc
     1141 acacgatcag taagcgcctt tgccgaaaaa gaccacaggg atcgttttca gcagaatcaa
     1201 gatgtcattg aacaacgaca tattctgaac atagtacagg tcgtactcca gattggcatg
     1261 aaggggaagg tctttgcgtc caatgatctg ccagagtccg gtgataccag gtttgaccaa
     1321 caggcgccga cgctgccaga cgtcgtattt ttcaacgata aagggcattt ccggacgagg
     1381 gccgaccatg ctcatatctc ccttgagcac gttgatcagc tggggaatct cgtccaggct
     1441 ggagcgtcgc aagaaccgtc cgaatccaaa cacgcgcgga tcatcgcttc gcgtcggggc
     1501 ttcttcatag ttgggcacat ccatgtacat cgtccgaaat ttgtacagga caaagatctt
     1561 gccacctttt ccaacacgct cctgcttgaa caggatggtg cccttggaac tgaggcgaat
     1621 acccagggc
//
LOCUS       DAOQUC010001051         1628 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_101665_length_1628_cov_3.206612, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001051 DAOQUC010000000
VERSION     DAOQUC010001051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1628
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101665_length_1628_cov_3.206612"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1536)
                     /locus_tag="PKH07_15460"
     CDS             complement(<1..1536)
                     /locus_tag="PKH07_15460"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNT36383.1"
                     /translation="MKHALVGSRQYLLIAMASTIVFGLLILLVLADQRHDMALCDQLV
                     EGQCQQLVNECSLRLQSEITERVNDLAILAVHLSMHGDKDEREQHFVRVASTVVADQP
                     AYHVLNYVDSDSIVRVSVPVGKRPELLGLDLKTLPGRTDLHRRVMESGEPLASPVMTL
                     TTGDPGLVIWYPIRNGLEDRNASGMVAGTFHLNTMLARCIPVNAEDHFQILLSQDGNQ
                     FHPSPPQISAKADSFQTQETCAQFILVGCYWKVTAEPLPVSPLARLPQRIKERGAVGF
                     VAASLFALLFGTTLTAFARARRSRAVALESEQRYRSLVDTMNEGLAIVDGSGVYTYVN
                     QLFCEILGRERDEVIGHCPVEFAAEESKTAAEHQIVARRQGNEDRFQIAVRRKSGELR
                     HIIVSPRLIRDSDGRFAGWLSVITDITEIKQTEQDREHLIRDLEAKNAELERFTYTVS
                     HDLKSPLITIKGFLGLVEAEALEGRFEEMKQDIARVDSAAGKMQDLLDQLLAVHRLGQ
                     KGKD"
BASE COUNT          378 a          448 c          426 g          376 t
ORIGIN      
        1 gtcttttcct ttctgaccaa gccggtggac agccagaagc tgatccagga gatcctgcat
       61 cttgccggca gcactatcta ctcgggcaat atcctgtttc atctcctcga agcggccttc
      121 caatgcctct gcctcaacca gtcccaggaa cccctttatg gtgatcaagg gactcttcag
      181 gtcgtgggaa acggtgtagg tgaatcgctc caactctgca ttcttggctt ccaagtcacg
      241 gatcaagtgc tcgcgatcct gttccgtctg cttgatctcg gtaatatccg tgatgacgga
      301 gagccagcct gcgaaacgtc cgtcggaatc gcggatcagc cgtggtgata cgataatgtg
      361 tcgcagttca ccagacttcc ggcgaacagc gatctgaaaa cggtcttcgt tgccttgtct
      421 gcgtgcaacg atctggtgtt cggcggccgt cttggattcc tcagcagcaa actccacggg
      481 gcagtgaccg atgacctcgt ccctctctcg accgagaatc tcgcagaaca gctgattgac
      541 ataggtgtac acaccactgc cgtctacgat ggcaagtcct tcgttcatag tgtccacgag
      601 tgaacgatat cgttgctcgc tctccaaagc gactgcacgg cttcgtctgg cacgcgcaaa
      661 agcagtcagg gtcgtgccaa acaacagtgc aaacagggac gcagccacga aaccgacagc
      721 gccacgttct ttgattcttt gaggcagcct cgccaaagga cttacgggca agggttctgc
      781 agtcactttc caataacatc cgaccaggat aaactgggcg caggtctctt gcgtttgaaa
      841 tgaatctgct ttggcggaaa tctgcggcgg agaaggatga aactggttgc catcctgact
      901 gagcagaatc tgaaagtgat cctcggcatt cacgggaatg caccgtgcga gcattgtgtt
      961 gagatggaag gtacctgcta ccattccact tgcgttgcga tcctcgaggc cgtttctgat
     1021 cggataccag ataaccagac ctggatcacc tgttgtcaac gtcatcacag gcgatgcgag
     1081 aggttcgccg gactccatca ctcttcggtg caaatcagtc cgacctggca gagtcttcag
     1141 atccagcccc agaagttctg gtctctttcc caccggaacc gagacgcgga cgatgctgtc
     1201 cgaatccacg tagttcagaa catgataggc gggctgatct gcgacaacgg ttgaggccac
     1261 acgcacgaag tgttgttctc gttcgtcttt gtcgccgtgc atggagagat gcacggccag
     1321 gatggccaga tcgttgaccc gctctgtaat ctcgctttgg agtctcagac tacattcatt
     1381 gaccaactgc tgacactgcc cctcgacaag ttgatcacag agagccatgt catgccgttg
     1441 gtcggcaagt acgaggagaa taagaaggcc aaagacgatt gtggatgcca ttgcgatcaa
     1501 caggtactga cgggaaccga caagtgcgtg tttcataccc gcatccatct cttcgcaaca
     1561 gacagcgaat cccaaacgtg gtcattatat agcacaacat attgcatttg tgaagtttga
     1621 ggagcagg
//
LOCUS       DAOQUC010001052         1626 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_101889_length_1626_cov_2.910885, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001052 DAOQUC010000000
VERSION     DAOQUC010001052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1626
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101889_length_1626_cov_2.910885"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            72..1109
                     /locus_tag="PKH07_15465"
     CDS             72..1109
                     /locus_tag="PKH07_15465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36384.1"
                     /translation="MFHRDRGLWCRMGLAVLFWAIVLVQPADAAVSIEEISEGTFPAC
                     YRVSNASVEVWVSRDFGPRILRYSLVGEENLFAERPDIVLKNEWGAYKLWGGHRIWTS
                     PEATPLTFAGDDQPVTLTKRVSDEETVFQFVGSAHPQTRLQQEIHLALEPEGTTARVR
                     QLIRNVDDESKEIAVWALTIMAPGGRVFIPQPSRQGRLTPARALVLWDYTDLSDPRWS
                     FGKTWMQLRAEDRFKGRQKIGLGNESGWLGYLKGKTIFTKVFPWSGRWEEYPDFGCNC
                     EVYSDGGFVELESLSPRKTLAPGEAIAHEEVWPLLPDVDAGETEESLSAALEDHLPRI
                     EQEARNLISTY"
     gene            1178..>1626
                     /locus_tag="PKH07_15470"
     CDS             1178..>1626
                     /locus_tag="PKH07_15470"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNT36385.1"
                     /translation="MNVVILCPDQLRADYLSCYGHPTIGTSHIDALASEGVLLERTYC
                     AAPLCGPSRISFVTSTRFSEHNHRTYGSTINFGVPNLVSSLKAAGFRTAMFGKNHCFN
                     EEQLPSIWDEMDNVCAGNYDRHPKYQRSFDAFELEADHEYNLTPRLTR"
BASE COUNT          377 a          408 c          450 g          391 t
ORIGIN      
        1 aagtggaaac gactccaggg ccacgtctct tttttgatat tctcagtggg acaacagaag
       61 ggggttccta gatgtttcat cgcgacagag gcttgtggtg caggatggga ttggcagtct
      121 tgttttgggc gattgttctt gtgcagcccg ccgatgctgc ggtctccatc gaggaaatca
      181 gtgaggggac atttcctgcc tgttaccgag tttccaatgc ttcggtggaa gtctgggtga
      241 gtcgggattt cggaccgagg attctccgat acagccttgt gggagaagag aacctctttg
      301 ccgagcgacc agacattgtt ctgaaaaacg agtggggagc ctacaaactt tggggtggtc
      361 accgcatctg gacatcaccc gaagccacgc ctctgacttt tgcaggggac gatcaaccgg
      421 tcacacttac aaaacgtgtc tccgatgagg agacagtgtt tcagtttgtc gggagcgctc
      481 accctcagac ccgacttcag caggaaatcc accttgccct tgagcccgaa ggaacaacgg
      541 caagagtcag gcaactcatt cgcaatgttg atgatgagtc caaagagata gctgtctggg
      601 ctttgacgat catggcacct ggtggcaggg tcttcatacc ccaaccttcc cgacaaggac
      661 gattgacacc tgcccgtgcc ttggtgctct gggactatac cgatctcagt gatccgcgct
      721 ggtcgtttgg gaaaacgtgg atgcaacttc gggccgaaga tcgcttcaag ggaagacaga
      781 agatcggtct gggcaatgaa tcgggttggc tgggctatct caaaggaaaa accatcttca
      841 ccaaagtgtt cccatggtcg gggcgctggg aagagtatcc ggatttcggc tgcaattgtg
      901 aagtctacag tgatggaggc tttgttgaat tagagagtct cagtcctcga aagactttgg
      961 caccgggcga agcaattgcc catgaagagg tgtggccgct gcttccggat gttgacgctg
     1021 gtgaaaccga agagagtttg agtgctgcct tggaagacca tctccccaga atcgagcaag
     1081 aggctcgtaa tctgatctca acgtattgag agttgatcga aagtcgtcta ttcttgtttg
     1141 aactgcacca gggtcatttc caaggaggcg gtcattcatg aacgtcgtca ttctttgtcc
     1201 tgatcaactt cgagcggact atctttcctg ctacggtcat ccgacgattg ggacgtccca
     1261 cattgacgct ctggcatccg aaggagtcct gctcgagaga acctattgtg ccgcgcccct
     1321 gtgtggtccg agccgaatca gctttgtcac ctcaacccga ttctccgaac acaatcatcg
     1381 aacctacggt tcaacgatca actttggtgt gccaaacctg gtgagttcgc tcaaggccgc
     1441 aggattccgc actgcgatgt ttggcaagaa tcactgcttc aatgaagagc agctgccatc
     1501 catctgggat gagatggaca acgtctgcgc tggcaactat gaccgtcatc cgaagtatca
     1561 gcgctccttc gatgcctttg aactggaagc agatcatgag tacaatctga ctccaagact
     1621 gacccg
//
LOCUS       DAOQUC010001053         1624 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_102120_length_1624_cov_2.955386, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001053 DAOQUC010000000
VERSION     DAOQUC010001053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1624)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1624)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1624
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102120_length_1624_cov_2.955386"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..803)
                     /locus_tag="PKH07_15475"
     CDS             complement(<1..803)
                     /locus_tag="PKH07_15475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009253518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNT36386.1"
                     /translation="MAQERKPNIVLVLADQLRYQSCGYAGDVKARTPNIDAFAHEGVN
                     FTNAVSGHPVCAAFRASLWTGKFTTSTGMVINELRMNPNHECLAHVLTQNGYETGYIG
                     KWHLWANQLGNHNDPKNSYIPPGEHRLGFDGFWAAYNFHHHYYKGYYHTNSSEQIPVP
                     GYEPDFQTDLAIDFIEKHSHSKNPFALVLSVGTPHDPWGADNVPPEYYDLFADEAGTP
                     RFSVPPNFKPEDDPYSDNWGRFKEPDRLQAIPRMLRGYYGMIANLDWNFG"
     gene            complement(974..>1624)
                     /locus_tag="PKH07_15480"
     CDS             complement(974..>1624)
                     /locus_tag="PKH07_15480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017365785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36387.1"
                     /translation="DAAARRQILSAPSRAIRFVGSSEATILKTELDGMVTRYESEILL
                     AALIENVNVRGRVIEYLIAGEDERLRQELIASLNSGATGLPSFKTPNALGDYQRRFDL
                     FDTETDVKTKIMIWNSNPKAYNLDKMLEFLARDRSVFMFYFVGVDPVRIVNTVLVSMF
                     QKDLLDATVLLKHWAGRNSRGVSQFEGKAINALIEKPDDQIDEALAQQFLTRIMAL"
BASE COUNT          388 a          418 c          416 g          402 t
ORIGIN      
        1 ccaaagttcc agtccagatt ggcgatcatg ccgtagtatc cgcgaagcat cctcggaatc
       61 gcctgaagtc ggtctggttc cttgaagcga ccccagttgt cagagtaagg atcatcctca
      121 ggcttgaagt ttgggggaac cgagaaacgc ggcgtacccg cctcatcagc aaacaggtca
      181 taatactccg gtggcacgtt gtctgctccc cacggatcat gcggtgtacc aaccgagagc
      241 accaacgcaa aaggattttt cgaatgcgaa tgtttctcga tgaagtcaat ggcaagatcg
      301 gtctgaaaat caggttcata gccgggcact gggatctgtt cggagctgtt ggtgtgatag
      361 tagcctttgt agtagtggtg atggaagttg taagcagccc agaacccgtc aaagcccagg
      421 cggtgctcac cggggggaat gtacgaattt tttggatcgt tgtggttgcc aagttgattc
      481 gcccacagat gccacttgcc gatgtagccg gtttcatatc cgttttgcgt caacacgtgg
      541 gcaaggcact cgtgatttgg attcatgcga agctcattga tcaccatgcc ggttgacgtc
      601 gtaaatttgc ctgtccacag cgaggcgcga aacgcagcgc acaccggatg tcccgaaacg
      661 gcgttggtga agttgacacc ttcgtgggcg aaagcgtcaa tgttcggcgt tcgcgccttg
      721 acatcacccg catagccaca ggactgatac cgaagttgat ccgccaatac cagaacaatg
      781 ttgggttttc tttcttgagc catattcctt ccttttctca atgccagaaa cgggagggca
      841 cacgatccga gcagaaactc tcggcgatga agacgggggg aagatgcgtt cgtttgctcc
      901 atatgattca ccggtgcaga gccgagggca caaccgtact acctacgtct tctggattaa
      961 ccatgtccat ttttcagagt gccattatcc gagtgagaaa ctgctgagca agagcctcat
     1021 caatctggtc atccggcttc tcaatcagtg cattgattgc tttcccttca aactgcgata
     1081 cgcctcggga atttcgtccc gcccagtgct tcagcagtac tgttgcatcc agcaaatctt
     1141 tctgaaacat cgaaacaagg acggtgttga caatccgcac gggatcaacc ccaacgaagt
     1201 agaacatgaa cacagagcga tctctggcga gaaactcaag catcttgtca agattatagg
     1261 ctttcgggtt tgagttccat atcatgatct ttgtctttac atctgtctct gtgtcaaaca
     1321 gatcgaaccg tctctggtaa tctcccagcg cgttgggtgt cttgaacgag ggaagaccgg
     1381 ttgcccctga attgagggag gcgatgagtt cttgcctcag tctctcatct tccccagcga
     1441 tcaagtactc gatgacacga cctcgaacgt tcacgttctc aatcagagcg gctaacaaga
     1501 tttcactctc atatctagtg accatcccgt cgagttccgt tttgaggatt gtggcttcgg
     1561 atgacccgac aaatcgaata gcacgactgg gagccgaaag gatttgcctt cttgcagcag
     1621 catc
//
LOCUS       DAOQUC010001054         1622 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_102317_length_1622_cov_3.082323, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001054 DAOQUC010000000
VERSION     DAOQUC010001054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1622
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102317_length_1622_cov_3.082323"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1305)
                     /locus_tag="PKH07_15485"
     CDS             complement(<1..1305)
                     /locus_tag="PKH07_15485"
                     /inference="COORDINATES: protein motif:HMM:TIGR03296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M6 family metalloprotease domain-containing
                     protein"
                     /protein_id="HNT36388.1"
                     /translation="MKTDFVRRCKILGLFTFSCCLGALLISQTAHAVIANPDPETLQQ
                     PNGVTFLAQQGGDEWLNWVVSDGTLIEKDSDGYWCYLAKSDKEPIRSKARVGMAPVPT
                     QALSVTEFLQLESSQRRVPQGARIASENIAAPSGPTERILLILVSFNDRSITTSSSAW
                     QNALFGTSGKTVRTYYNEVSKGAFYFVPAEETYGTANDGVVSVTLNRAHPNTADNTDD
                     RNRQIVADALVAADPYVNFASFDTSGNGSISGAELHIVTIIAGYERAYSSTVSPSIWA
                     HRWSLWGAVAPVTLDGKSVGGGGYTQQGELHGNTSGSHQATIGVLCHELGHDIGLPDL
                     YDTDASNGDSYGIGIFGLMAGGSWGNTSGEYSGATPTHMCAWSKIAMGFVIPTVITSG
                     GTQTLYRSNNSGYNVVRLNTSDTNQYFLIENRHTEGFDAGMYR"
BASE COUNT          397 a          418 c          429 g          378 t
ORIGIN      
        1 acgatacatg cccgcatcga agccttccgt atgacgattc tcgatgagaa aatactggtt
       61 tgtgtcgctt gtgttcagac gaacaacgtt gtatcccgaa ttgttgctgc gatagagggt
      121 ctgcgtaccg ccggaggtga tcacggttgg aatgacaaaa cccatggcaa ttttcgacca
      181 tgcacacata tgcgttgggg ttgcaccgga atactcgccg ctcgtgtttc cccaagaccc
      241 tcctgccatc aggccaaaaa tgccgatacc gtaagagtcc ccattggatg cgtctgtatc
      301 atagagatcg ggcagaccga tgtcatgtcc cagttcatga caaagaacac caatggtcgc
      361 ctgatgactt ccagaggtat tcccatgcag ctctccctgt tgcgtgtagc caccgcctcc
      421 aacgctcttg ccatccagcg tgaccggcgc gacggctccc caaagagacc agcgatgtgc
      481 ccagatactc ggtgaaacgg tgctggaata ggcgcgttca taacctgcaa tgatcgtgac
      541 aatgtgcaac tccgctcccg aaatgctgcc attgccagag gtatcaaatg aagcgaaatt
      601 gacgtaggga tctgccgcaa cgagcgcatc agcaacaatc tgacggttcc tgtcatcggt
      661 gttgtcggct gtgtttgggt gggctctgtt gagagtgaca ctgacaacac catcattggc
      721 tgtgccgtag gtttcctctg ccggaacgaa gtagaaggct cccttggaaa cctcgttgta
      781 gtaggtgcgc actgtcttgc cggatgtgcc gaagagggca ttttgccacg cggaagaaga
      841 agtcgtgatt gagcgatcgt taaaactgac cagtatgagg agaattctct ccgtcggtcc
      901 cgaaggagcg gcgatgttct ccgaagcaat tctggctccc tgaggaacac gacgttggct
      961 ggattccaac tgcagaaatt ccgtgacaga gagcgcctgt gtgggaaccg gtgccatgcc
     1021 gacacgggct ttgcttctga tcggctcttt gtccgatttt gccaggtaac accaatatcc
     1081 atccgagtcc ttttcgatga gcgttccatc tgaaacaacc cagttgagcc attcatctcc
     1141 accctgttgg gcgaggaaag tgacgccatt gggctgctga agtgtttctg gatcggggtt
     1201 ggcgatcacg gcatgtgccg tctgtgagat caggagcgct cccagacaac acgaaaaggt
     1261 gaacagtccc agaatcttac aacgtcggac aaaatcggtt ttcacggcag ttccttcctc
     1321 tcaggtcatt tcagtccaaa tcctgatagg ggttattgca cggacaggga ttttgtgtca
     1381 actgcaacaa catggaactg tttggtgtat aactgtccca tggaaaagcg accacaaaga
     1441 tgagaggaac accaatgccc aagctcaaat ggaatacaca tgcagatctt gctatcaaga
     1501 aaactccgac agtcggtcgt gaagagtatc tcgactacat gatcttcaga cgcaatgacc
     1561 gccctctgtt taccgagata ttcggccctt tgatcggtct gaaggaagag tgggaagaac
     1621 ag
//
LOCUS       DAOQUC010001055         1621 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_102419_length_1621_cov_3.167944, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001055 DAOQUC010000000
VERSION     DAOQUC010001055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1621
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102419_length_1621_cov_3.167944"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..263)
                     /locus_tag="PKH07_15490"
     CDS             complement(<1..263)
                     /locus_tag="PKH07_15490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HNT36389.1"
                     /translation="MDVLEPVIRFFRQQRELGQDSIHLEQPLSKVCSKEEQLRKIQDQ
                     CQNCRLCQLWKGRTQIVFGDGDVNARIVVVGEAPGRDEDIQGIP"
     gene            complement(294..1397)
                     /locus_tag="PKH07_15495"
     CDS             complement(294..1397)
                     /locus_tag="PKH07_15495"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HNT36390.1"
                     /translation="MAINVNTMLEKMLEMNASDLHLKVGSPPMVRVDGTLRPHPQNNE
                     PLKPDDTLDIAQQLIMTNKRLKIPEIGSIDFSYAIPGVARYRVNVFHQRGTISLVLRM
                     VKFQVPPFDELGLPAVIREIAKYERGLVIVTGVTGSGKSTTLASIIDHINETRSAHIV
                     TIEDPIEFLYRDRQCIINQQELGLDFETFSDALKRALRQDPDIILIGEMRDQETIRAA
                     ITAAETGHMVLSTLHTMDATQTVDRVLKYFDTDEQELIRMQLSLNLKAVISQRLLKRA
                     AGRGRVPAVEVLVATPVVQKLIFEGRTKELKAAITSRESGMQSYNQALVELVKKGTVA
                     KEEALLYVDNPQAFERNLKGGYSDGDKGGLIGF"
     gene            complement(1411..>1621)
                     /locus_tag="PKH07_15500"
     CDS             complement(1411..>1621)
                     /locus_tag="PKH07_15500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36391.1"
                     /translation="LAGEELRHLPFYDYAVVNNTIEESVELIRNIIVAERCRRERVLH
                     RLACANSALRNWVGAAISKPVDTVK"
BASE COUNT          378 a          440 c          391 g          412 t
ORIGIN      
        1 gggattccct gaatatcctc atcccgcccg ggtgcctctc ccacaacgac aatacgagca
       61 tttacatctc catccccaaa gacaatctgc gtgcgtcctt tccagagctg gcacaggcga
      121 cagttctgac actggtcttg tatctttctg agttgctctt ctttggagca caccttcgac
      181 agaggttgct caagatgaat agagtcctgt cccagctcgc gctgttgccg gaaaaatcgg
      241 atcactggtt ccaatacatc catgtgcctt atcttcccaa tgcgcaaaga atcctagaac
      301 ccaatcaggc cacccttatc gccgtctgaa taacctccct tgaggtttcg ttcaaacgcc
      361 tgcgggttat ccacatagag tagggcttct tccttggcga cggtgccttt cttgaccagt
      421 tcaaccaagg cctggttgta actctgcatg ccactttcgc gactcgttat ggccgctttg
      481 agttccttgg tgcgtccctc gaaaatcagt ttctgcacaa caggtgtggc aacaagaacc
      541 tcgacggcag gcactcggcc tctgccggca gctctcttga gaagtcgctg ggagataacc
      601 gctttgaggt tcagcgacag ctgcatgcgt atcagttcct gttcgtccgt gtcgaaatac
      661 ttcaatactc ggtcaacggt ttgcgtagcg tccatcgtgt gtagcgttga gagaaccatg
      721 tgtcctgtct ccgcagcggt aatcgcagcc cgaatggtct cctgatcacg catctcgcca
      781 atgagaatga tatcgggatc ctgtcggagt gcgcgcttca gggcatcgga gaaagtctca
      841 aaatccaatc ccagttcctg ttgattgatg atgcactgtc tgtcccggta gagaaactca
      901 atgggatctt cgatggtgac gatgtgcgcc gaacgggtct cgttaatatg atcgataatg
      961 gaagccagag tggtggattt tccgctgcct gtcactccag tgacgatcac aagaccacgt
     1021 tcatattttg cgatctcccg gatgacagca ggcagaccaa gttcgtcaaa aggaggaacc
     1081 tggaacttca ccatacgaag cacaagactg atggtgcctc gctgatggaa aacattcact
     1141 cggtaacgtg caacaccagg gatcgcgtag gagaaatcaa tgcttccaat ctcaggaatt
     1201 ttgagcctct tgtttgtcat gatcagttgt tgagcaatgt ccagagtgtc atctggtttc
     1261 agaggctcgt tgttctgagg gtgagggcgc aacgttccat ccactcgcac catcggggga
     1321 ctgccaacct tcagatgtag atccgatgca ttcatttcga gcatcttctc aagcattgtg
     1381 ttcacattga ttgccatggt tcatcctccc ctacttgaca gtatccaccg gtttcgaaat
     1441 ggcagcaccc acccagttgc gaagcgctga attggcacag gcaagcctgt gcaaaacacg
     1501 ttctcgacga cagcgttcag caacaatgat attccggatc aattccaccg attcttcgat
     1561 agtattgttt acgacagcat agtcatagaa aggcaaatga cggagttcct cacccgccag
     1621 g
//
LOCUS       DAOQUC010001056         1618 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_102741_length_1618_cov_2.765835, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001056 DAOQUC010000000
VERSION     DAOQUC010001056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1618
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102741_length_1618_cov_2.765835"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1618
                     /locus_tag="PKH07_15505"
     CDS             <1..>1618
                     /locus_tag="PKH07_15505"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNT36392.1"
                     /translation="GSMTPEEICDRLLETIRPAIPLDAFFVSRYDAASDKTYPIRTYD
                     TFDNVLTRVTPTRKAIAVSKLIEPAVRERKPILIHRDPKELLDVNTLGFGDAQRPSLS
                     LIFVPFIVQDRVPGIFSVQSYTPNAYTKDDLELMMAIAQQVGPAVEASVLSQELRRSE
                     EAQRKFGERLTALVEVANELALEDSSDDLFRRSVELGASVLGFERLSIWLLGDTPDTL
                     MGTFGIGTDGAVKDKREEMIQIVDGSLFVPLLQNSTSFVLCQDAPLFEDKTIVGKGAV
                     AAATLRAKDKIVGLAVADNLLRHSPLENHDGQILHLFCSVVGSLYLDKDREKQLRETT
                     ALLIHNERMNALGELTAGVAHELNQPLNNVKLLCQSILRDMDKDRLNADELPQDLRDV
                     VSQINKMAGIIDHMRVFTRRSDRVHKQRIALNDCINNMFILLGQQLRTHGISVRTELA
                     EDLPLISGDSIALEQVATILVVNARQAVEEFRTSDRSIEIKTFQFSPTEVALAVGDNG
                     GGVPVALRKRIFEPFFTTKAAGKGTGLGLSI"
BASE COUNT          376 a          422 c          439 g          381 t
ORIGIN      
        1 cggaagcatg actccagagg agatttgcga ccgactcttg gagaccattc gtcctgccat
       61 tcctttggat gctttcttcg tcagtcgtta cgatgcggcc tccgacaaga cctatcccat
      121 aagaacctat gacacatttg acaacgttct gacaagggtc acgcccacaa gaaaggccat
      181 cgccgtctcg aaacttatcg agccagcagt tcgcgaacgc aaaccgatcc tcatccacag
      241 agatcccaag gaactgttgg atgtcaacac tcttggtttt ggtgacgctc agagaccttc
      301 gctctccttg atctttgttc cgttcattgt gcaggacaga gtccctggca tcttctccgt
      361 gcagagttac accccaaacg cttacacgaa ggatgatctg gaattgatga tggcgatcgc
      421 tcagcaggtg gggccagccg ttgaagcgtc tgttttgagt caggaacttc gccgcagtga
      481 ggaagcgcaa aggaaatttg gggagagact cacggctctg gtcgaggttg ccaatgaact
      541 ggcactggaa gactcctccg atgatctctt tcggcggtcg gtcgagttgg gagcatcggt
      601 gctgggtttt gagaggctga gtatctggct ccttggggac acgcccgaca cactcatggg
      661 aacatttggc atcggaaccg atggagcggt caaggacaag cgagaagaaa tgattcagat
      721 tgttgatggg agtcttttcg tgccgcttct ccagaactcc acgtcatttg ttctttgtca
      781 ggatgcaccg ctttttgagg acaagacgat cgttggcaag ggtgccgttg cggcagccac
      841 tttgcgtgcc aaagacaaga tcgtcggtct ggcggtagcg gataacctac tccggcactc
      901 cccacttgag aaccatgatg gtcagatcct ccacttgttt tgctctgttg tgggttctct
      961 gtatctggac aaggacaggg agaagcaact tcgagagaca accgcactgc tgattcacaa
     1021 cgagcggatg aacgcactcg gagagttgac tgccggcgtg gcgcatgaac tcaaccagcc
     1081 tctgaacaat gtcaaacttc tctgccagag cattctgcgc gacatggaca aagaccgtct
     1141 caatgctgac gaactcccac aggacttgcg tgacgttgtg tcgcagataa acaagatggc
     1201 aggcatcatt gaccacatgc gtgtcttcac gcgacgatcc gaccgcgtcc acaaacagag
     1261 aattgcgctg aacgactgca tcaacaatat gttcattctt ctgggtcagc agcttcgaac
     1321 ccatggcatc tcggttcgga cggaactggc agaggatttg cctttgatat cgggcgattc
     1381 cattgctttg gaacaggtcg caaccattct ggttgtcaac gcgcggcaag ccgtggaaga
     1441 gtttcgcact tctgaccgct cgatagagat caagaccttt caattcagtc caacggaagt
     1501 ggctctcgct gtcggtgaca atggtggggg tgtccccgtg gcgctccgca aacggatatt
     1561 cgaaccgttc tttacaacca aggctgcagg caaaggtact gggcttggtt tgagcatc
//
LOCUS       DAOQUC010001057         1618 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_102760_length_1618_cov_2.335253, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001057 DAOQUC010000000
VERSION     DAOQUC010001057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1618
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102760_length_1618_cov_2.335253"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..969
                     /locus_tag="PKH07_15510"
     CDS             <1..969
                     /locus_tag="PKH07_15510"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNT36393.1"
                     /translation="VLAALWFESLSAAIFLLLFLAIQSTAFGPAKYGMIPELMPEECL
                     GRANGVISMMTNLAIITGVIIGGLLSEGYPGSRAMPGLVMLGAAVSGYVASLFMEKLP
                     ARDPKSKVTANPMRPYLDTLKIMARRRYLLDIAIGLGFFGFIGIMVMQAILDFKVLLN
                     LSDRQASYLNVPIMLGVAVGSLTAGYLSKAKVRIGLVPIGALGMALVLGILGTHQLTT
                     VRVVVGLVSMGFFGGFYVVPLEALLQGRSPLEIRGRVQGTKAFLDFGLIALGGAAYKV
                     LRGTLDLNVQMVLVLCSIITLVVAGYMFWSLRRYLKQRIGQILGSS"
     gene            complement(999..>1618)
                     /locus_tag="PKH07_15515"
     CDS             complement(999..>1618)
                     /locus_tag="PKH07_15515"
                     /inference="COORDINATES: protein motif:HMM:NF014788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HNT36394.1"
                     /translation="VEKGRGDGIFITTSGIGLTRSDVQISTESIVAGDALILTGTLGD
                     HALAVLKARNQFSLDFEIESDVAPVNGLVATLLDAVPETRFLRDPTRGGVAAVLGETS
                     RDKAWGFILDEQALPITSQVRSLCEILGYDPLTLANEGKVVAVVPADAVEKSLKALQS
                     HPLGRHAAKIGYVDTVRPGIVLLKTLIGGTRVVQVPLGEDLPRIC"
BASE COUNT          330 a          430 c          449 g          409 t
ORIGIN      
        1 gtgctggctg ccctgtggtt tgaaagtctt tcggcagcga tctttcttct tctgtttttg
       61 gcaatccaga gtacggcgtt tgggcctgcc aagtacggga tgataccgga gttgatgccc
      121 gaggagtgtc tcggtcgtgc caacggtgtc atcagcatga tgaccaatct tgcaatcatc
      181 acgggggtga tcatcggtgg tttgctgagt gaaggatacc cgggctccag agccatgccg
      241 ggtctcgtca tgcttggtgc tgcagtgtcc ggctatgtgg catccctttt catggagaaa
      301 ctgccggcac gtgaccccaa gtccaaggtg actgccaacc caatgcgacc ttatctggac
      361 acactcaaga tcatggcgcg acgaaggtat cttctggaca tcgccatcgg cctgggattc
      421 ttcgggttta ttggaatcat ggtcatgcag gcgattctcg atttcaaggt acttctgaac
      481 ctttctgaca ggcaggcaag ttatctcaac gtgccaatca tgctgggtgt ggcagtcggc
      541 agtctgacag caggttatct atccaaggca aaggttcgca ttggcctggt tcccatcgga
      601 gcgttgggaa tggctttagt tttgggaatt cttggaacgc atcagttgac tacagtccgt
      661 gttgttgttg ggcttgtgtc catgggtttc ttcggtgggt tctacgttgt cccgcttgag
      721 gctcttcttc aaggccgttc tcctttagag attcgtggtc gagttcaggg aaccaaagcg
      781 tttctggatt ttggcctgat tgctctggga ggagcggcct ataaagtcct gcgaggcacg
      841 ctggatctca atgtccagat ggtgttggtt ctatgctcga tcatcactct ggttgtggcg
      901 ggatacatgt tctggagcct tcgcagatac ctgaagcagc gaatcggaca gatcctggga
      961 agctcgtaag acggattcat cacaagccga gatgagcatc aacagatccg cggcagatcc
     1021 tcgcccagcg gaacctgcac gacacgcgtg cctccgatca gtgtcttgag cagaacgatg
     1081 cccggacgaa ctgtatcaac gtacccgatc ttcgccgcat gtctgcctaa gggatggctc
     1141 tgaagcgcct tcagtgactt ctcgacagca tctgcgggga caacagcaac aaccttccct
     1201 tcattggcaa gcgtcaacgg atcatatccc aggatttcac acagactccg gacttgcgaa
     1261 gttattggaa gcgcctgctc atccaatatg aagccccatg ctttgtctcg gctggtttct
     1321 cccagcactg ccgccactcc accacgggtc ggatctctga ggaatcgggt ttccggaact
     1381 gcgtcgagaa gtgtcgccac caagccgttc acgggtgcca catcgctctc gatttcgaaa
     1441 tccagggaga actgatttcg agccttgaga acggccaagg catgatctcc cagcgtgccc
     1501 gtgaggatta gggcatcccc tgcaacgatg ctctctgtcg agatctgcac gtcagatcgc
     1561 gtcagcccga ttccagaggt tgtgataaag attccgtcac cgcgaccttt ctcaacgg
//
LOCUS       DAOQUC010001058         1617 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_102828_length_1617_cov_3.158771, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001058 DAOQUC010000000
VERSION     DAOQUC010001058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1617
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102828_length_1617_cov_3.158771"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1093
                     /gene="murA"
                     /locus_tag="PKH07_15520"
     CDS             <1..1093
                     /gene="murA"
                     /locus_tag="PKH07_15520"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HNT36395.1"
                     /translation="CCFGIETKREVGGIFRVEPKTLRWFEAPYDLVRKMRASFFVLGP
                     LVAKSGQARVSMPGGCAIGRRPVDIHIKALEKLGVEIAVSHGYVEAHGLELRGAEIVL
                     DFPSVGATENVLMAATLAKGESVIRNAAREPEVSDLANFLVAMGAKIEGIGTDVLHVQ
                     GVQSLSGGAYRVIPDRIEAETFMVAGAMTGGDVTITDATPDHCTSVIEKLRQIGARLD
                     VTDGKIRVQGPDEFKPVEVSTMPYPGFPTDSQAQLMALLTIAKGTSIVKETIFENRFM
                     HVSELVRLGADITIDGNCAIVKGVPNLSGSPIMASDLRASAALILAGLIARGETEVLR
                     VYHLDRGYEHIEAKLSALGANVERFRRGQ"
     gene            1229..>1617
                     /locus_tag="PKH07_15525"
     CDS             1229..>1617
                     /locus_tag="PKH07_15525"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HNT36396.1"
                     /translation="MSTFVILVFLLGASAFFSASETALFSLPKTRIEGFRRDRRRLGR
                     AVWELLSKPSNLLITILIGNMTVNILSSSLSAHFLTGLFGKEGLALSIVGMWFLILVC
                     GEVTPKIIAIRQNERLAPYMAPASRSD"
BASE COUNT          324 a          423 c          466 g          404 t
ORIGIN      
        1 gtgctgcttc ggcatcgaaa ccaagcgcga ggtggggggt atctttcggg ttgagcccaa
       61 gacccttcga tggtttgagg caccctatga cctggttcgc aagatgcgcg catcgttttt
      121 cgttctcggc ccgctggttg ccaagagcgg tcaggcacgg gtctcgatgc ctgggggatg
      181 cgccatcggc cgccgtcctg ttgatattca tatcaaggct ctggagaaat tgggtgtgga
      241 gatcgccgtc tcccatggct atgttgaagc gcatggtctc gaacttcggg gagcggagat
      301 cgttctggat tttccgagtg ttggagcgac ggaaaatgtc ctcatggctg ccactttggc
      361 caagggagag agtgtcattc gaaatgcggc acgcgaacca gaagtgtccg atctggccaa
      421 ctttcttgtt gccatgggag cgaaaattga gggcatcggc accgatgttc tccatgttca
      481 aggtgtgcag tccctatctg gtggagctta tcgggttatc cccgaccgca ttgaggccga
      541 gaccttcatg gtcgctggtg ccatgaccgg aggcgatgtc acgatcacgg atgcgacccc
      601 cgaccactgc accagcgtga tcgagaaact caggcagatc ggagccaggt tggatgttac
      661 ggatggcaag attcgggttc agggtcccga cgagttcaag ccagtggaag tttctaccat
      721 gccttatccc ggttttccga ctgacagtca ggcgcaactg atggcgcttt tgaccatcgc
      781 caagggtacc agtatcgtca aggagacaat ctttgaaaac agattcatgc acgtctctga
      841 attggttcgt cttggcgcag atatcaccat tgatggcaac tgcgccattg tgaaaggcgt
      901 tccaaacttg tccggctccc cgatcatggc gtccgacctt cgggcgagtg ccgccttgat
      961 cctggctggg ctcattgcgc gaggcgaaac cgaggtgctt cgcgtgtacc atctggatcg
     1021 aggatacgaa catatcgaag ccaaactgtc cgcactgggc gccaatgtcg agcgctttcg
     1081 acgcggacag tgaaaaatcc ggagcaatcc aggctcttga taagggcagg tgttcccacc
     1141 tctcgattca tttgcctgag tgcattcagg tcattatact cttcagtaga gggtctctga
     1201 tcccgatttg gatttgggtg aagaagagtt gtctacattt gtcatcttgg tctttcttct
     1261 tggtgcgtca gcgtttttct ccgcttctga gacggcactc ttctcccttc cgaagacgcg
     1321 cattgaaggc tttcggcgtg atcgaaggcg tctgggaaga gcggtctggg aactgctctc
     1381 caaaccctca aatcttctaa tcaccatcct catcggcaat atgaccgtga acatcctctc
     1441 ttcctccctg agtgcccatt ttctgacagg gctgtttggc aaggaaggcc tggcgctgtc
     1501 catcgtcggg atgtggttcc tgattctggt gtgcggggaa gttactccaa agatcatcgc
     1561 gattcgtcag aacgagaggc tggcgccata tatggcgcca gcctctcgtt ctgacga
//
LOCUS       DAOQUC010001059         1616 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_102894_length_1616_cov_4.659193, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001059 DAOQUC010000000
VERSION     DAOQUC010001059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1616
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102894_length_1616_cov_4.659193"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..578
                     /locus_tag="PKH07_15530"
     CDS             <1..578
                     /locus_tag="PKH07_15530"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HNT36397.1"
                     /translation="LGIVDDDQVELSNLQRQICHSTPDCGRLKTDSAKQRLLELNPDV
                     VIEAFPVRFTPTNAESLLGEWDFVLDATDNFEAKFLIADACHRLGKSYSHAGIARFLG
                     QTMTVVPGKTACYRCLFDTQPDFAPDDPPKGPMGPVPGVIGSLQAMEAIKFLVGCGNL
                     LTDRILTYNALTAQFREIRVKRNPECRLCHS"
     gene            759..>1616
                     /locus_tag="PKH07_15535"
     CDS             759..>1616
                     /locus_tag="PKH07_15535"
                     /inference="COORDINATES: protein motif:HMM:NF012475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36398.1"
                     /translation="MTIIEFEETLVSWRMQKYRHPKIDAIKEGFRPVSGRVADFGVAE
                     KDGRYHFYYIERRLQEGTPFYPGNEIYLGHASTADFLDWEVHDLVMLIRPNTWEEAHV
                     WAPSLLYHKGRYVMAYTGINRYISQDIGLAFSDDLFNWERDPRNPISPCKGKDWAFWH
                     EDRISSCRDPHIFEHEGRVWIAYTANTRQGASCIALMSSEDLTRWEDHGPILVGPSEG
                     YEPRLEGGHPQGSLESCNLLTRNGKWYLLVNASIRGETSRNWVFESDRMDAFDFSNRR
                     EFWNGAGGIE"
BASE COUNT          365 a          426 c          451 g          374 t
ORIGIN      
        1 ggctgggcat tgtagacgat gaccaggtcg aactgagcaa cctgcaacga cagatctgtc
       61 attcgacgcc ggactgtgga cgcctgaaga ctgactcagc caaacagcgt ctgctcgagc
      121 tgaatcccga tgttgtgatc gaggcttttc cggtacgatt cacgccgaca aatgccgaga
      181 gtcttcttgg cgaatgggat tttgtgctgg atgccactga caacttcgag gcgaagtttc
      241 tgattgcgga cgcttgtcac cgtctcggga aatcttactc acacgcagga attgcccggt
      301 ttttgggtca gacgatgacg gtggttcctg gaaagaccgc ctgttaccgg tgcctttttg
      361 acacgcagcc agacttcgcg ccggatgatc cacccaaagg gccgatgggt ccggttcccg
      421 gagtcatcgg ttcccttcaa gccatggaag cgatcaagtt tttggtcggc tgtggtaatc
      481 ttttgacaga tcgaattctg acctacaacg cgctgaccgc tcagttccga gagattcggg
      541 tcaaacgaaa tcccgaatgc cggctctgcc attcgtaacg tgggagtcac cccacgttcc
      601 tttcttcaag cccgcggcat gaatgcctca ctacacagaa ccgtcctgaa cgaaaccgga
      661 agccgcttct ccatgcgatt gcctcgaagc gtgttcgatg tgctacacta ccggcaggat
      721 tcgacacctg taagttttga ttgggaggcc gcaaaagaat gaccatcatc gagttcgagg
      781 agactttggt ttcctggcgc atgcagaaat accgtcaccc aaagatcgat gccatcaagg
      841 agggctttcg ccctgtcagc ggtagagtgg ctgactttgg agttgcggag aaggacggtc
      901 ggtaccattt ctactacatc gaacggcgtc ttcaggaagg cacgcctttc tatcctggca
      961 atgagatcta tctgggtcat gcttcgaccg ctgacttcct cgactgggaa gtgcatgatc
     1021 tcgtgatgtt gatccgtccg aacacatggg aagaagcaca tgtttgggca ccgtcccttc
     1081 tgtaccacaa gggtcggtat gtgatggcct atacgggcat caaccgttat atctcccagg
     1141 atatcggtct tgccttcagc gacgatcttt tcaactggga acgcgaccct cgaaacccca
     1201 tctcaccctg caaaggcaaa gactgggcgt tctggcacga ggatcgaatc tccagctgtc
     1261 gcgaccctca catctttgag catgaaggca gggtttggat cgcctatacg gctaacacac
     1321 gccaaggggc ttcgtgtatc gccttgatga gttcggaaga tctgacccga tgggaagacc
     1381 acggaccgat ccttgttggc cccagtgaag gctacgaacc aaggctggaa ggtggacatc
     1441 cgcagggcag tctcgaatcg tgcaatctcc tcacaagaaa cggtaaatgg tatttgttgg
     1501 tgaatgccag cattcgggga gagacgagtc ggaattgggt ttttgagagc gatcggatgg
     1561 acgccttcga tttctcgaac cgccgagagt tctggaatgg cgcaggaggc atcgag
//
LOCUS       DAOQUC010001060         1614 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_103107_length_1614_cov_3.612572, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001060 DAOQUC010000000
VERSION     DAOQUC010001060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1614
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103107_length_1614_cov_3.612572"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="PKH07_15540"
     CDS             <1..224
                     /locus_tag="PKH07_15540"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="catalyzes the formation of L-threonine from
                     O-phospho-L-homoserine; Derived by automated computational
                     analysis using gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HNT36399.1"
                     /translation="VHVAEDFGLEGDPLVCVSTAHPAKFPEAIREATGESVARHPDID
                     ALAGLPTRCEILDNDSGAVRAFIEKTLSA"
     gene            278..>1614
                     /locus_tag="PKH07_15545"
     CDS             278..>1614
                     /locus_tag="PKH07_15545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015254409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HNT36400.1"
                     /translation="MAIKVAMIGAGSIGFTRTLMRDILTVPELQDTLFAFTDINRRNL
                     DIVTQLAKRDITENKVPARILSTTNRKKALEGADYVFSLVRVGGLDAFKLDIDIPLKY
                     GVDQCVGDTLAPGGIMYAQRTIPVLLDFCRDIREVSKPNALFMNYSNPMAMNTWACMK
                     YGGVNSVGLCHGVEGNHAIIAEALGIPWRELDVTAAGINHQTWFIRVMHEGRDMLPKL
                     LEAFRRHPVYSKSEKVRIDMLERFGYFSTETNGHLSEYVPWYRKRPKEIRQWIDLSIW
                     PGGETGGYLRSCLETRDWFETEAPKWLKEPVRAFSPESRSREHGSHIIEAMETGRIYR
                     GHFNVRNQGIITNLSQGCIVEVPGYVDGFGINTPVIGELPLACAATCEASVRVQEMGM
                     EAAVSGNITLLKQAVLHDPLTAAVCNPPEIWQMVDEMLVAQAQWLPQYRREISA"
BASE COUNT          372 a          386 c          473 g          383 t
ORIGIN      
        1 gagttcatgt tgccgaggat ttcggtttgg aaggcgatcc actggtctgt gtttcaacgg
       61 cacatccggc aaagtttcca gaagccattc gagaagcgac tggtgagtcg gttgctcgtc
      121 atccagatat tgatgctttg gcgggactgc cgactcggtg cgagatactg gacaacgact
      181 cgggtgcggt tcgagccttc atcgagaaga cactgtcagc atagcgacgt ttagacgcga
      241 tggattggca gcctgcgcgc tgcaaaagga gagttcgatg gcaatcaaag tagcgatgat
      301 cggagccggg agtattggat tcacgcgaac actgatgcgg gacattctta cagttcccga
      361 gctgcaggac accctctttg catttacgga catcaacaga cgcaatttgg atattgtgac
      421 tcagctggca aagcgggata ttaccgagaa caaagttcct gctcgaattc tgtccaccac
      481 caatcgaaag aaggcacttg agggagccga ctatgttttc tcgctggtgc gtgtgggagg
      541 cttggatgcc ttcaaactcg atattgacat ccccttgaag tatggcgttg atcagtgcgt
      601 tggcgataca cttgcccctg gcgggatcat gtacgctcag cgaacgattc ccgtgcttct
      661 ggatttctgt cgggacattc gcgaggtttc caaacccaat gcgctcttca tgaactactc
      721 aaatcccatg gcgatgaaca catgggcgtg tatgaagtat ggaggggtga attctgtcgg
      781 tctctgccac ggtgtggagg gaaaccatgc gattatcgcg gaagcactgg gcattccgtg
      841 gcgtgaactt gatgtgacgg ctgcaggtat caaccatcag acttggttta tcagagtcat
      901 gcacgagggc agagatatgc tccccaaact gcttgaggca ttcaggaggc atccagtcta
      961 ttccaagtcg gagaaagttc gcattgatat gttggaacgc tttgggtatt tcagcacaga
     1021 gaccaacggg catctttcgg aatatgttcc ctggtatcga aagcgtccca aggagattcg
     1081 tcagtggata gatctctcca tctggccggg gggtgagacc ggaggctatc tgcgcagttg
     1141 cctggaaacc cgagactggt tcgaaaccga ggctcccaag tggctcaagg aacccgttcg
     1201 agctttctca ccagagtctc gttcgcgtga gcatggcagc catatcatcg aagccatgga
     1261 gacaggtcga atctatcgtg gccatttcaa tgttcgcaac caggggatta tcacgaatct
     1321 ttctcaaggg tgcattgtcg aagtgcctgg ctatgtggac ggttttggca ttaacactcc
     1381 cgtgatcgga gaactgccct tggcttgtgc cgcaacgtgt gaggcaagtg ttcgggttca
     1441 ggaaatggga atggaagcgg cggtcagcgg taacatcacg ttgctcaagc aggcagtgct
     1501 gcatgatccc ttgacggcag ccgtgtgcaa cccgcctgag atttggcaga tggtggacga
     1561 gatgctcgtc gcgcaggcgc agtggctacc tcaatatcgc agggaaatat cagc
//
LOCUS       DAOQUC010001061         1614 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_103115_length_1614_cov_3.200128, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001061 DAOQUC010000000
VERSION     DAOQUC010001061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1614
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103115_length_1614_cov_3.200128"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(159..845)
                     /locus_tag="PKH07_15550"
     CDS             complement(159..845)
                     /locus_tag="PKH07_15550"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HNT36401.1"
                     /translation="MLPFPKGLVVSCQSNPGDALHGSHFMQAMAVAAQKGGAVAIRAN
                     GPADIAAIKAVTALPVIGIWRIYDPGCEVYITPTFESAQVIRSAGAEVIAFDATSRKR
                     PKSLTLAELVRQLKALNTPLMADISTYEEGCRAAELGVDIIATTLAGFTGGQRMEHGA
                     PDLNLLQQLIANVNLPVIAEGRYTTPELVRQAMQMGAYAVVVGKAITQPEFIVRKFVQ
                     GVSGCQSVSG"
     gene            complement(853..1266)
                     /locus_tag="PKH07_15555"
     CDS             complement(853..1266)
                     /locus_tag="PKH07_15555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36402.1"
                     /translation="MSLKWPICPECLKEEEEILTQVQHYLRDHPKESLDEVVEALDVD
                     KALIMKFIREKRLIISAGSSVRLTCSSCGAPVLSGTLCRACQARLTGGASPSSESKET
                     TKPSESQAPKSTMTDSRFRSHGGGSSSIKDKFQRG"
     gene            complement(1386..>1614)
                     /locus_tag="PKH07_15560"
     CDS             complement(1386..>1614)
                     /locus_tag="PKH07_15560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36403.1"
                     /translation="FRNGGYESSTMTLSAGYSYKFTLFEDNDPFNMFDTVKDVKISYY
                     SGTGEEVLEQESWIFKREALGIWRIIEMSLP"
BASE COUNT          364 a          478 c          392 g          380 t
ORIGIN      
        1 ggcgagcagg aaaacgatcc cccagacaaa cagagccgtc catcttccgg cttcagcatc
       61 tcttgtttct ttcagaaccg acaaacgcat ttgcggtctc ctttcctgtc ctacggtaca
      121 aaaccagaaa ccattattgt gaagagtggc gatgcaagtc aacctgaaac cgactggcac
      181 ccgctcacgc cttgcacaaa tttccgcaca atgaattcag gctgcgtgat cgccttacca
      241 actacaacgg cataggctcc catctgcatc gcctgacgca ccagctccgg agtcgtatac
      301 ctgccttcag ctatgacagg caggttgaca ttggcaatca gttgctgcaa aagattcaga
      361 tcaggggctc cgtgctccat cctctgtcca ccggtgaatc cagccagcgt cgttgcgatg
      421 atatccactc caagttctgc agcccgacag ccctcttcat aggtcgaaat atcggccatc
      481 aagggagtgt tcagagcctt caactgccgc accaattccg ccagcgtcaa actctttggc
      541 ctcttcctcg atgttgcgtc gaaagcgatc acttcggctc cagcactccg gatcacctgt
      601 gccgattcaa aggtgggcgt gatgtagacc tcgcagcccg gatcatagat gcgccagata
      661 ccgatcaccg gaagtgcagt caccgctttg atcgctgcaa tgtctgccgg accattggct
      721 cgaatcgcaa ccgccccacc cttctgcgcc gccactgcca tcgcctgcat gaaatgtgat
      781 ccatgcagcg cgtcacccgg attggactga caagaaacga caagtccctt cggaaatggg
      841 agcatggcct gctcagcctc tttgaaactt gtccttaata ctggaactcc caccaccatg
      901 ggagcggaat ctgctgtcgg tcatggttga cttcggcgct tggctttcac ttggtttggt
      961 ggtctctttg gattcggaag acggagaggc acctcctgtc agtcgggctt gacacgccct
     1021 gcagagggtt ccggaaagaa ccggtgctcc gcaggaactg catgtcagac gtactgaaga
     1081 acccgcagag atgatcagcc tcttctctcg gatgaacttc atgatgagtg ccttgtccac
     1141 atcgagagcc tcaaccactt catccagcga ttctttggga tggtcccgaa gatagtgctg
     1201 gacttgagtc agaatctcct cctcctcctt gagacactcc ggacagatag gccatttcag
     1261 gctcatgaac agcttgttgc atttgcgaca gttgataagt gccatggttg atagtggtga
     1321 ggccggcggg tccgccggcc tcgatatcct ttctcaaagt gtcgtcccgt ccgcagcgga
     1381 tctgcttagg gaagcgacat ctcgatgatc cgccaaatcc ccaaggcttc tcgcttgaag
     1441 atccaagact cctgctcgag aacctcttcg ccggttccac tatagtagga tatcttcaca
     1501 tccttgactg tgtcaaacat attgaacgga tcattgtcct cgaacagggt gaacttgtaa
     1561 ctgtagccag cgctgagtgt cattgtggag ctctcatagc caccattcct aaag
//
LOCUS       DAOQUC010001062         1613 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_103223_length_1613_cov_3.252888, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001062 DAOQUC010000000
VERSION     DAOQUC010001062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1613
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103223_length_1613_cov_3.252888"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            184..711
                     /locus_tag="PKH07_15565"
     CDS             184..711
                     /locus_tag="PKH07_15565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HNT36404.1"
                     /translation="MLLVHICCAPCLTYPHEWLQERKVKFKGLFFNPNIHPFQEFRKR
                     AETLEEYSAKMGIDIAYEKRYGLLDFLHSTVGREKERCLFCYESRLRRAASEAKAQGF
                     SAFTSTLLVSPYQKHDLIKSTAERVAQEEGIEFFYVDWRDGYRQGVQRSLDLGLYRQR
                     YCGCIYSEAEREGAL"
     gene            711..>1613
                     /gene="ftsY"
                     /locus_tag="PKH07_15570"
     CDS             711..>1613
                     /gene="ftsY"
                     /locus_tag="PKH07_15570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014904484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HNT36405.1"
                     /translation="MFGQGLLKKLKDKLVVTHKTLVEKAESLFRRHGKIDQELLDELE
                     EALIEADVGVDTSVSLVEELREAAKKSERLGEEEDIDWLRRTLKQLLAERLQEGDTEL
                     RFAKEGLSVFLIVGVNGTGKTTAIGKLANRYVQQGKQVVLAAADTFRAAAMEQLEIWG
                     ERAKAAVIRGRPGTDPASIVYDAIQAGRARNADIVIIDTAGRLQTKANLMRELEKIGR
                     VIKREMPEAPHETLLVLDATTGQNGLVQAKVFAEAVPLTGLILTKLDGTAKGGVSIAI
                     KDQLKLPIKFIGVGEGLDDLEVFDK"
BASE COUNT          395 a          375 c          478 g          365 t
ORIGIN      
        1 cccagtggca tttccattct tcaggtagac gcctcaaggc ctcttgatgt tcctctgcct
       61 gaagtgacgg tgggactgcc tccacagtga tgggctgtga tgagaagagt cctgtcggat
      121 atgacaggcc agagccgctt gccatcttgg taagaccggt acacgtgaaa ggagcctgag
      181 agtatgctgc tcgtccatat ctgttgtgcc ccatgcctga cctatcctca tgaatggctc
      241 caagagagga aagttaagtt caaaggcctt tttttcaatc cgaacattca tccgtttcag
      301 gagtttcgga agcgtgctga gactctggag gagtattcgg cgaagatggg cattgatatt
      361 gcttacgaga agcgatacgg cttgctcgat ttcctccatt caacggtagg cagggagaaa
      421 gaacggtgcc tattctgcta tgaatcgcga ctgcgcaggg cagcatcaga agccaaggct
      481 cagggcttct cagcgtttac gtcaaccctt cttgtgagtc cctatcagaa acatgatttg
      541 atcaagtcaa ccgcggagcg tgtcgcccag gaagaaggca tcgagttttt ttatgttgac
      601 tggagagacg gttatcggca aggtgtccaa cggtctctag atctgggact ctatcggcag
      661 cgatactgcg gttgtattta tagtgaagca gaacgggagg gtgctctctg atgtttgggc
      721 aaggactact caagaaactt aaagacaaac tggttgtcac gcacaagact ctggttgaga
      781 aggcggaaag cctctttcga cggcacggta aaattgacca ggaactcctt gacgagttgg
      841 aagaagcgct catcgaagcg gatgtcggtg tggatacatc tgtcagcctg gtggaagagt
      901 tgcgtgaggc ggccaagaag agtgaaaggc tgggcgaaga ggaagacata gactggcttc
      961 ggagaacgct gaaacaactt cttgcggaac ggttgcagga gggggacact gaactgcgtt
     1021 ttgcgaagga aggtctgagc gtttttctca tcgtcggtgt gaacggaacg ggaaagacga
     1081 ctgcaattgg caaactcgca aatcgctatg tccagcaagg caaacaggtt gttctcgccg
     1141 cggcagacac tttccgcgcc gcggccatgg aacagctgga gatttggggt gagcgcgcaa
     1201 aagcagcagt gatccggggg cgacctggta ccgatccggc atctatcgtc tatgacgcga
     1261 ttcaggcggg aagagccaga aatgcggaca tcgtcatcat tgataccgcc ggcaggcttc
     1321 agaccaaggc aaatctgatg cgtgaacttg agaagattgg ccgtgtgatc aagcgggaaa
     1381 tgcctgaagc accccatgag acgctcttgg tactcgatgc tacaacgggt cagaacggac
     1441 tggttcaggc caaggtcttc gcggaagccg tccctttgac aggcttgatc cttaccaaac
     1501 tcgatggtac tgccaaaggc ggtgtttcca tcgccatcaa ggatcagctg aagcttccca
     1561 tcaagtttat cggggtcggt gagggtcttg acgacctcga ggttttcgac aag
//
LOCUS       DAOQUC010001063         1613 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_103225_length_1613_cov_3.166239, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001063 DAOQUC010000000
VERSION     DAOQUC010001063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1613
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103225_length_1613_cov_3.166239"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="PKH07_15575"
     CDS             complement(<1..189)
                     /locus_tag="PKH07_15575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36406.1"
                     /translation="MMKFNLFAMLFLACFLVATCSFAQTDTPTDTPTETPTDTPTPTV
                     TDTPTDTPTPTVTDTPTET"
     gene            complement(524..>1613)
                     /locus_tag="PKH07_15580"
     CDS             complement(524..>1613)
                     /locus_tag="PKH07_15580"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HNT36407.1"
                     /translation="MNSVDPQTGEVIRFHDEVVGLTEALASQAAVAITNASLIEETKA
                     LFESLIRVMATAIDAKSLYTGNHIERVALLNLFLARAINEKTEGPLAGVQFSEKELEE
                     IRLAGWLHDVGKITTPEWVMDKATKLQAIYDRIGLIRMRYAFIKESVKACSPRSDQCG
                     KVQETSTTGDEELPARLDELDQEIQFLEQCNKPSEFLDDEKLARLGQIAQKTFEFESE
                     THARLTENELYNLSIRKGSLTSEEIQVMKDHVVETRKMLSQIPFKGHLKNVPLYAAQH
                     HEKLNGTGYPDGLHEQDIPIQSRILAVADFYEALVAKDRPYKKPMPLEVAFSILRKAA
                     QNGEIDSDILDILIEDKVYERLEKGKEG"
BASE COUNT          365 a          414 c          405 g          429 t
ORIGIN      
        1 cgtctcggtt ggtgtatccg tcaccgttgg tgtgggcgtg tcggtaggag tatcagtcac
       61 cgttggcgtc ggtgtatcgg taggggtctc cgtcggcgtg tcggttggcg tgtcggtttg
      121 cgcaaaagaa catgtcgcca ccagaaaaca cgccagaaac agcatagcaa ataggttgaa
      181 tttcatcatg ataagttcag taggtgctcg caattaccgc tgtccaatgc ccgtaaaaga
      241 gcgcctcgtt cctccttgga gtggaaataa accaatataa taacccatag taactcatac
      301 tatgcgtata gtatcatcca cgttcccaaa ctgtcaacaa ttccttcatt tttcggggcc
      361 atcggctgcg ggcacgcgag ttatgtccac tctcgtgaac tgcttgttcg ggaaatgaac
      421 cgtttcatca gaaatccggc ttcttgagag actcgtctgg ctgagaatcg aacagaaatc
      481 tgcaagcgtt gatggccgag gaaagagaac acgcttggga agactatcct tccttccctt
      541 tttcaagacg ctcatagacc ttgtcttcaa tcagaatgtc aaggatatct gagtcaatct
      601 ccccgttttg ggcagccttt cgaaggattg agaaggccac ttccagcggc atgggcttct
      661 tgtaggggcg gtctttggca acgagtgcct catagaagtc agccacggca agaattcgcg
      721 actgaattgg aatatcctgt tcgtgaagac catctggata gcctgttcca ttgagtttct
      781 catgatgctg ggcggcgtag agtggaacgt tctttagatg acccttgaaa ggaatctggg
      841 agagcatctt tcttgtctca acgacatgat ctttcataac ctgaatctct tcggacgtca
      901 ggcttccctt gcgaatcgag aggttgtaca attcgttttc cgtcagccgt gcgtgagtct
      961 cgctctcaaa ctcaaaggtc ttctgagcga tctgacccag tcgggcgagc ttctcatcat
     1021 caagaaactc tgagggcttg ttgcactgtt ccaaaaactg tatttcctgg tcgagttcat
     1081 caagtcttgc gggaagttct tcatcgcccg tagtcgaagt ctcctgcacc tttccgcatt
     1141 gatccgatct tggtgaacaa gccttcacag attctttgat gaaagcgtac cgcatcctga
     1201 tcagaccaat ccggtcgtat atcgcctgga gttttgtcgc cttatccatc acccattcgg
     1261 gtgtggtgat cttgccgaca tcatgaagcc agcccgccaa acggatttcc tccagctcct
     1321 tctcagagaa ttggactccg gccagtggcc cttctgtctt ctcattgatc gcccgtgcca
     1381 gaaagaggtt caggagcgcc acgcgctcaa tgtgatttcc tgtatatagg gattttgcat
     1441 caatggccgt cgccataacg cgaataaggg actcaaagag cgccttggtc tcttcgatga
     1501 gactggcgtt ggtgatcgca actgccgcct gagaagccag cgcttccgtc aatccaacaa
     1561 cctcgtcgtg gaagcgtatg acctcgccgg tctggggatc aacggagttc atc
//
LOCUS       DAOQUC010001064         1612 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_103353_length_1612_cov_3.012845, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001064 DAOQUC010000000
VERSION     DAOQUC010001064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1612
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103353_length_1612_cov_3.012845"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(76..1476)
                     /locus_tag="PKH07_15585"
     CDS             complement(76..1476)
                     /locus_tag="PKH07_15585"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HNT36408.1"
                     /translation="MKTRLRKTLILAACLVIIFELTVLGMRHLRAYTLRANVVLILLD
                     TVRADRLGCYGNSEGLTPNIDRFAEDSVRFEYAYSHAPWTLPSVASLYTSRHPMKHGA
                     GGDINSFTILPDSAQTLAEILRDAGAKTAAVVNVSFLTKTFGLHQGFGTMNVGREVGN
                     EVCRTADQTTDAALTWLSQNGKKRFFLVVHYFDPHLIYDPPKEYRMRFADAVDRDTEG
                     TLFGSRQQMLAFRSGKLKLDEPLVRRLEKLHNAEIAFVDAQIGRLLSTLAEMKISDET
                     VIVITSDHGEEFWDHGGFEHGHTLYHELLHVPLILHIPSGVDTRSIELKGKTVSLPLR
                     HIDVAPTLCQLVGLETPDDFDGMAIDITSPDTEPTRPVLAQGNMWGPAGVSYQYDGHK
                     VIQEASPGSTLLFDITDDPMETRDISQASPELTEKMVSLMNRLLDEQRHANAETDKAL
                     LTETEKERLRSLGYMK"
BASE COUNT          418 a          409 c          400 g          385 t
ORIGIN      
        1 ctctagcaga acaacgagag tgccttcctt cgcaaatgca ctcaacttca ttcctggaag
       61 tcctcgacgc caacgctact tcatgtatcc gagagaacgc aggcgctcct tctcagtctc
      121 ggtcaacaag gccttatctg tctccgcatt ggcatggcgc tgttcatcaa gaagacggtt
      181 catcagagag accatcttct ctgtcaactc tggacttgcc tgactgatgt cccgtgtctc
      241 catggggtca tctgtaatgt caaaaagtaa ggtgcttccg ggacttgcct cttggataac
      301 tttgtgtcca tcgtactggt acgacactcc ggcgggcccc cacatattac cctgggcaag
      361 aacaggcctt gtcggttcag tatctggtga cgtgatgtcg attgccatac catcaaaatc
      421 atctggagtc tctagcccta ccaactgaca caaggttgga gcgacatcaa tgtgccttaa
      481 gggcaaagac actgtctttc ctttgagttc aatggaacga gtgtcaactc cagaggggat
      541 atgaagaatg agaggcacat gaagaagttc atggtaaaga gtatggccat gctcgaaacc
      601 accatgatcc cagaactctt cgccgtggtc agaggtgatg acgataaccg tctcgtctga
      661 aatcttcatc tcagcaagcg tgctgagaag ccttccaatc tgggcatcca caaacgcaat
      721 ctcagcgttg tgaagtttct ccaaacgacg aacaaggggc tcatccagtt tcaactttcc
      781 tgaacgaaaa gccaacatct gctgacgcga gccgaagaga gttccttccg tgtctctatc
      841 tacggcatca gcaaatctca tccgatactc ttttggtggg tcatagatca aatgaggatc
      901 gaagtagtga acaacgagga agaagcgctt cttcccattc tggctgagcc aagtcagagc
      961 ggcatcagtt gtttgatcag cagttcggca gacttcattt ccaacctcgc gtcccacatt
     1021 catggtgcca aaaccctgat gaagtccaaa agtcttcgtc aggaagctca cattgacaac
     1081 tgcagcggtc tttgcacctg catctctaag aatctcagca agagtctgcg ccgaatcagg
     1141 gagtatggtg aaagagttaa tgtcccctcc agcaccatgc ttcattggat gacgtgaggt
     1201 gtagagagat gccacagaag gaagagtcca gggagcgtgg gagtatgcgt actcgaaacg
     1261 gactgagtct tctgcgaatc ggtcaatatt tggggtaagg ccttctgaat ttccatagca
     1321 gcccaagcgg tcagctcgga ccgtgtcgag aagaatgagc acgacatttg cccggagcgt
     1381 gtatgctcgg agatgcctca tcccaaggac tgtgagctca aaaatgataa caaggcaggc
     1441 agcaagaatg agtgtcttcc tcagtcgggt cttcactgaa tctccttggt tcctggttaa
     1501 gacgcaagaa cttctctgac ctgctcacag tgctctggat caagggaaaa ggttcctgca
     1561 tgcgcgttag actccaattg ctgaaccgat gaacaggatg gaatgatggg ac
//
LOCUS       DAOQUC010001065         1612 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_103370_length_1612_cov_2.684650, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001065 DAOQUC010000000
VERSION     DAOQUC010001065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1612
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103370_length_1612_cov_2.684650"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..985)
                     /locus_tag="PKH07_15590"
     CDS             complement(<1..985)
                     /locus_tag="PKH07_15590"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNT36409.1"
                     /translation="MWDSFADRLFERPLLIVLFAAILLVAGLYAWKNIPIDAFPDVTN
                     VQVMILTQTPGLTPDEVERLITNPIEVQMGGLPGVDSVRSLSRSGLSQIVVIFTDDTD
                     IYFARQLVLERLVSARESLPEGADPELGPISTGLGEIYQYVVEAGYYCLAHPREWSEQ
                     QGTCSHCSAELVATNYNATDLRSFQDWIIAPQIRRIPGVNEVNSFGGYVKQIHVIPEP
                     EQLLKYDISLTSILESLDANNANVGGSFLLKDWEQSYLVSRGLLQDETDIQQIVLKTW
                     EGTPLYLGDVANVRIGGQERQGAVTMDGKGEVVAGMVIMLKGMNSERVVARV"
     gene            complement(1003..>1612)
                     /locus_tag="PKH07_15595"
     CDS             complement(1003..>1612)
                     /locus_tag="PKH07_15595"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNT36410.1"
                     /translation="GRLTLQSPCDGTIVARNTTEGALVTPETVLFRIADPSQLWVWCQ
                     LYERDLESLHSLLDKSSSVSAHLQTLAFPDVKFPGVVDLIASELDPQTRTIPVRVRVE
                     NQDGKLRPGMFATVTIPVKTQQTALCIPKDAVVSDEGVDFIFKHWKEDYWVRQDVKLG
                     RSFGETIEVLGGLESGDWIVVSGAFLLKSDLLREKMGAGCAD"
BASE COUNT          366 a          456 c          403 g          387 t
ORIGIN      
        1 tgacccgggc aaccaccctt tctgagttca tgcctttgag catgataacc atgcccgcaa
       61 cgacttcacc ctttccgtcc atggtcacgg caccctgccg ttcctgaccg ccaatgcgga
      121 cgttggcgac gtcgcccaga tagaggggcg ttccctccca agtcttcaga actatctgct
      181 ggatgtctgt ctcatcttgg agaaggcctc ttgaaaccag gtaactttgt tcccagtctt
      241 tgaggaggaa gctgcccccg acgttggcgt tgtttgcatc gagcgattcg aggatgctcg
      301 taagtgatat gtcatacttc agcagttgct cgggttccgg gatgacatga atctgtttga
      361 catatcctcc gaaactattg acctcattga cacctggtat tcttcgaatc tgtggagcaa
      421 tgatccagtc ctgaaaagag cgcaagtctg ttgcgttgta gtttgttgcg acgagttctg
      481 cactgcagtg tgaacacgta ccttgctgtt cagaccactc ccgcgggtgc gccaaacagt
      541 agtatccggc ttcgacgaca tattgataga tctcgccgag tccagttgat atcggtccca
      601 gttcgggatc ggctccttcc gggagcgatt cgcgagctga aaccaacctc tccagcacca
      661 attggcgcgc gaagtatatg tctgtgtcat ccgtaaagat gacgacgatc tgggagagtc
      721 cactgcgcga caaggaacga acggaatcta ctcctggaag ccctcccatc tgaacctcga
      781 tggggttggt gatgagccgt tccacctcgt cgggtgtcaa gcctggggtc tgagtcagaa
      841 tcatgacctg gacgtttgtt acgtctggga aagcatcaat ggggatgttc ttccaggcgt
      901 acaaacctgc aaccagcagg atcgccgcaa agagaacgat caacaatggc ctttcaaaca
      961 gtctgtctgc aaaagagtcc cacatcggat gaatccctcc gtttagtctg cacagcctgc
     1021 gcccatcttt tccctcagaa gatcactctt caggagaaaa gctcccgaaa cgacaatcca
     1081 atcgccactc tccagacctc caagcacttc gatggtttcc ccaaaggatc tccccagctt
     1141 cacatcctga cgaacccaat agtcttcctt ccagtgcttg aaaatgaaat caaccccttc
     1201 gtctgagacc acggcatcct ttgggatgca caacgctgtc tgctgtgtct tgaccggaat
     1261 ggtgacagtt gcgaacatgc ctggtcgcag tttgccatcc tggttttcca ccctgactcg
     1321 gactggaatt gttctcgtct ggggatccaa ctcgctcgcg atcagatcga cgactcccgg
     1381 aaacttgacg tccggaaagg cgagagtctg aaggtgcgca ctgaccgacg aagacttgtc
     1441 aagaagactg tgaaggctct ccaggtcgcg ttcgtacaac tggcaccaca cccaaagttg
     1501 agaaggatcg gcgattctga aaagaacggt ctccggagtg accagtgcgc cttccgttgt
     1561 attccttgcc acgatggttc cgtcacaagg tgactgtagt gtcagccttc ca
//
LOCUS       DAOQUC010001066         1611 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_103485_length_1611_cov_2.550129, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001066 DAOQUC010000000
VERSION     DAOQUC010001066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1611
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103485_length_1611_cov_2.550129"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(109..>1611)
                     /locus_tag="PKH07_15600"
     CDS             complement(109..>1611)
                     /locus_tag="PKH07_15600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36411.1"
                     /translation="ICNSNNCPTPGCKSCQGISRRQFMHRSSLVGASLALGTSLSPWN
                     SSLADELRETVDIASLRPKPLVRVFGAIVRPTPPYWLGWPGTSYDVEGHLRDYQDAFS
                     KAAEKVGVQLALEPSPLESDDAVLAFVHKLQEEKPDAVLVSLQHMWAWGWVDRICQAG
                     IPSIIFAPVGTCFTGHVAEISRRPGIHLISSLEVRAVEQALRMVRAKRQFEESRLLVL
                     AGDQRNESVLERLGTKVRYIPRDALRTRFDQMPETDEVRRIARQIKRSAKRIVEPNRQ
                     DILNAARTFVTAKQLVRDEMSNAITTDCLGMVGSKVVPTPPCISVTLFHDAGVTYGCE
                     ADVFGAISLMFTSYLFDKPGFMNDPVPETFKNVLVAAHCACGTRLNGFDEKPEPLILR
                     THSESDLGVSAQVVWRIGQPVTLVRFEGPHQLILDTGTVVGNVNTPPAGGCRTSFEIA
                     MDKVEDARDVLGFHQVVFYGDHRRDVEAYCQMYGIKVVNSPERSQERRTL"
BASE COUNT          368 a          468 c          437 g          338 t
ORIGIN      
        1 cctgagaggt cacttcagtc ggcggcgtgt agggatctcc aattcgcacc tgcgcgctcg
       61 cagtgagagt aagaagagcc atcagaaagg caatgagcag ttggatcctc atagcgttct
      121 cctttcctgg gaacgttcgg gagagttgac cactttgatt ccgtacatct ggcaataggc
      181 ttcgacgtcc cttcggtggt cgccgtaaaa gacaacctga tgaaatccca gcacgtcgcg
      241 ggcatcctcc accttgtcca tggcgatctc gaaactggtt cggcatccac cggcaggcgg
      301 agtgttgaca tttccaacca cggttccggt atcgagaatc agctgatgcg ggccctcaaa
      361 acgaaccagg gtgacaggct gaccaattcg ccagacaacc tgtgctgaca cgcccagatc
      421 cgactccgaa tgtgttcgaa ggatcaacgg ttccggcttc tcgtcaaaac cgttcagacg
      481 agtcccacag gcgcagtgtg cagcgacaag aacattcttg aacgtctccg gcactgggtc
      541 attcatgaag cccggcttgt cgaaaaggta acttgtgaac atcagggaga tcgcaccaaa
      601 cacatctgcc tcacatccat aggtcacacc ggcatcatga aaaagtgtaa cggaaatgca
      661 cggcggcgtg ggaacgacct tgctgcccac catgccaagg cagtctgtgg tgatggcatt
      721 ggacatctcg tcacgaacca actgcttggc agtcacaaaa gtccgcgcag cattgagaat
      781 gtcctgtcgg tttggctcga cgatgcgctt ggcacttcgt ttgatctgac gcgcgatcct
      841 tctgacctca tcggtctctg gcatttggtc aaaacgtgtg cgcagggcgt cccttggaat
      901 gtaacggact ttcgttccca gtctttcaag cacactctca ttgcgctgat cgcctgcaag
      961 caccagaagc cgcgactcct caaattgcct cttggctcgc accatgcgaa gtgcctgctc
     1021 gaccgctcgg acttccaatg aggagatgag atgaatcccc gggcgtcggg agatttccgc
     1081 aacatgaccg gtgaagcagg ttccgaccgg cgcgaagatg atcgaaggaa tgcccgcttg
     1141 acaaatcctg tccacccagc cccatgccca catgtgttga aggctcacaa gcacggcgtc
     1201 cggcttttcc tcttgcagtt tgtgaacaaa ggcaaggact gcatcatcac tttcgagagg
     1261 ggacggctcc agcgccagtt ggacgccaac cttctcggct gccttggaga aggcatcctg
     1321 gtaatcgcgc aaatgtcctt cgacgtcgta agatgttcca ggccatccaa gccagtacgg
     1381 cggcgtcggt ctgactatcg ctccgaacac tctgacgaga ggcttcgggc gcagcgaagc
     1441 gatatccact gtctctctga gctcatcggc aaggctacta ttccacgggg acagtgacgt
     1501 ccccaaggcc aacgacgcac cgaccaacga actgcggtgc atgaactgac gccgagagat
     1561 gccctggcat gacttgcagc ctggggtagg acagttgttc gagttgcaga t
//
LOCUS       DAOQUC010001067         1609 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_103667_length_1609_cov_3.677606, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001067 DAOQUC010000000
VERSION     DAOQUC010001067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1609
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103667_length_1609_cov_3.677606"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(227..>1609)
                     /locus_tag="PKH07_15605"
     CDS             complement(227..>1609)
                     /locus_tag="PKH07_15605"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HNT36412.1"
                     /translation="RFTRPSSPPLSEIGAPVRLAGFHVGEVTGIELIPGQRRAQVKIM
                     LSEKYRLPEGSRARIDTSGIMGEKYIELSFGPGPGFYLPNDIIEGEEPFKMEDVVNKG
                     QAIADEVRTLVGSLNLILGEETTRTSLHALVKNLENLTANLDAVVGGERQNIHTIMSE
                     LTQASQNLSSALIQAESAMFTLGGLVEENRDEVQKTIENVKDFSDGLRNRGDDILVRF
                     DSALRRIDSVGQNLDRLTTQLNGLVGDSSPQVQEIVTSVKDSSRNLQEASGSAKRMLK
                     DLEDGRGVMGRLLRDEALGEKTERTLNQVSDTVNAWHNTLSSASLRYRFRGYRDREGL
                     GLDEDSSNRWRNDLTLGLNLGERNFLEVGGNDIGGDNDLELMFGRRFDRLSLRAGVKD
                     SEAAIGVDWLLIRDWFELSAEGIGLTDRREERLDLIGRLSVHDHVSIIGGVQDVGEGN
                     NANLGLELRY"
BASE COUNT          368 a          461 c          391 g          389 t
ORIGIN      
        1 tgtcaacagt tgcgtggaaa tgagtcgcca gagccatcag ttgtccgcgg aggggcgatt
       61 catgaattgc ccctaatcgt gaatcaccct tactgcacag ccatgacggt gttgattatg
      121 agtcggtagc aacgctgccg acgatgttca tgcggccttt ttcttctccc agtgggagag
      181 tttgaggatt ggaaggtgcg ttgtgcggat caagagccag cgatggtcag tacctgagtt
      241 ccagtccgag attggcgttg ttgccttcgc ccacatcttg aactccccca atgatggaaa
      301 catggtcgtg gacgctgagt cttccgatca gatcaagtcg ctcctcgcga cggtctgtca
      361 gaccgatgcc ctcggcggag agttcgaacc agtcgcgaat cagaagccaa tccacaccta
      421 tggccgcttc tgaatccttg acgccagcac gtaaggaaag tcggtcgaaa cggcgaccga
      481 acataagttc aagatcattg tctccaccga tgtcattgcc accgacttcc aggaagttcc
      541 gctcaccgag attgagcccc aaagtcagat cattgcgcca tcggttgctg ctgtcttcat
      601 ccaagccaag accctcacga tctctgtatc ctcgaaatcg gtaacgtagc gaggcactgc
      661 tgagcgtgtt gtgccaagcg ttgaccgtgt cgctgacctg attgagcgtt ctctcggtct
      721 tctctccaag cgcctcatcc cgaagcaaac gccccatcac accgcgtccg tcttcaagat
      781 ccttgagcat tctcttagcg ctgcccgaag cttcctgaag attcctggac gagtccttca
      841 ccgatgtgac aatttcctga acctggggac tggagtcccc gacaagacca ttcagttgcg
      901 tggtgagtct gtccagattt tgcccgacag aatcgatccg cctcaaagca ctgtcaaagc
      961 gaaccaggat atcatctccc cgattcctaa ggccgtcaga gaaatccttg acattttcga
     1021 ttgtcttctg gacttcatcg cgattctctt ccacaagacc acccagtgta aacatggcag
     1081 actcagcctg aatcaaggca ctgctgagat tctggcttgc ctgagtcagt tcggacatga
     1141 tcgtatgaat gttctgcctc tctccaccga cgacagcatc aagattggcg gtcaggttct
     1201 ccagattctt gaccaatgca tgaagagatg tccttgtcgt ctcctctcca agaatcagat
     1261 tcagtgagcc aacgagtgtt ctgacctcgt cggctatggc ttgacccttg ttcacgacat
     1321 cctccatctt gaaaggttcc tccccttcga tgatgtcgtt gggaagataa aaacccggac
     1381 caggaccaaa cgaaagctca atgtactttt cccccataat tccagaggtg tcaatgcgtg
     1441 cccggctgcc ctcaggcagg cgatatttct cagacaacat aatcttcacc tgagctctgc
     1501 gttgacctgg aatcaattcg atccccgtga cttccccgac gtgaaaaccc gcaagacgaa
     1561 cgggtgctcc gatttctgat aacggtgggg acgaaggcct agtgaagcg
//
LOCUS       DAOQUC010001068         1609 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_103671_length_1609_cov_3.447233, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001068 DAOQUC010000000
VERSION     DAOQUC010001068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1609
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103671_length_1609_cov_3.447233"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..163
                     /locus_tag="PKH07_15610"
     CDS             <1..163
                     /locus_tag="PKH07_15610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007903639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HNT36413.1"
                     /translation="HTPWIDGCVMPTVWKRKWGQGRVFYTALGHVAKDFEVPQLKEIV
                     KRGMIWASR"
     gene            211..1119
                     /locus_tag="PKH07_15615"
     CDS             211..1119
                     /locus_tag="PKH07_15615"
                     /inference="COORDINATES: protein motif:HMM:NF013885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36414.1"
                     /translation="MLRILGSRNFVFPFSIVLGLLLGEKAGWAKPFILPLLGLVMAVS
                     TTEIPTSVFLQFRQLVRATALTIVFNYLILGSVTLVLARWLMPDEPLWVGFVLFVAAP
                     PAVAVVPFTVVLGGNKALALLSTFGGYLASLALAPLITLLLLGGNIIPAAKLFWVLDE
                     LVIIPFVISRLILFVGFEKRLEDYRGTIVNWSFAIVVCAVVGLNRDVLVQEPSVFLLT
                     LLVGFVAPFLIGWAIDTTTKRLAIPEPDRKTLILMGTLKNSGFSAAVALSLVGARAAI
                     PSTMTSIFLTLYFLFLDFRFRKGTHA"
     gene            complement(1222..>1609)
                     /locus_tag="PKH07_15620"
     CDS             complement(1222..>1609)
                     /locus_tag="PKH07_15620"
                     /inference="COORDINATES: protein motif:HMM:NF024827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HNT36415.1"
                     /translation="GEAGGKCEARCTQSAQCLKCHVTTPSFTTEKQRAEGIGCERCHG
                     PGSLYSDSKTMKNRQEAVKKRLLPLKGQTEAETLANAEKQCRECHGLEHKDENPAAKE
                     FDFETAWPRVKHDRETLKENFPDKYK"
BASE COUNT          259 a          424 c          464 g          462 t
ORIGIN      
        1 gcacacgccg tggattgacg gatgtgtgat gccaactgtc tggaagcgaa agtggggcca
       61 gggtcgtgtg ttctatactg cgctgggaca tgttgcaaag gatttcgaag ttccccaact
      121 gaaggaaatc gtcaagcgcg gcatgatctg ggcgagtcgc tgatctttcg tctgctgaag
      181 catcgaacgg ctgaagggat gtgagcgcgt atgcttcgga ttctgggaag ccgaaacttc
      241 gtttttccgt tcagtatcgt tctcggactt ttgcttggag agaaggcagg atgggcgaag
      301 ccgttcatcc tgcctcttct cggtttggtg atggccgtct ccacgacgga gattccgacc
      361 tcggtctttc tgcagtttcg tcaactggtt cgcgcgaccg ctctcacgat tgtgttcaac
      421 tatctcattc tgggttctgt gacgctggtt ctggcgcggt ggctgatgcc ggatgagccg
      481 ctttgggtcg ggttcgttct ttttgttgca gcaccacccg ccgtggcggt ggttccgttc
      541 accgtagtgc ttggtggcaa caaggcgttg gctctcttga gcacgttcgg ggggtatttg
      601 gcttcgttgg cgttggcacc tttgattacc ttgctgttgc ttggcggaaa catcatcccg
      661 gcggcgaagc tgttttgggt gctggacgaa ctggtcatca ttcccttcgt aatttcgaga
      721 ctgatccttt ttgtgggttt tgaaaagcgc ttggaggact atcgcggaac tatcgtcaac
      781 tggtcgtttg ccatcgttgt ctgcgcggtc gtgggactga atcgggatgt ccttgttcag
      841 gagccgtccg ttttcttgct gactttgctg gttggttttg tggctccgtt cctcattggt
      901 tgggctattg acacaaccac caagaggctc gctatccctg agcctgaccg caagactctt
      961 atccttatgg ggacactcaa gaactcgggt ttctccgcgg cggttgctct gtccttggtc
     1021 ggtgctcgcg ctgccatccc atccacgatg acatccatct ttcttacgct ctatttcctc
     1081 tttctcgatt ttcggttcag aaaagggaca cacgcctgat acggtggatg catctgcggc
     1141 tcgaactctc gtaaacccga gactgacacg taagcgtgac gcttcttctc cacgatcagg
     1201 aatggatacg gcttgagagc gttacttgta cttgtctggg aaattctctt tcagcgtctc
     1261 gcggtcatgt ttgacccgtg gccaggcggt ttcaaagtca aactctttgg cggcgggatt
     1321 ctcgtccttg tgctccagcc catggcactc ccgacattgt ttctcggcgt ttgccaaggt
     1381 ctctgcctca gtctgtcctt tgagagggag aagccgcttt ttgactgcct cctggcggtt
     1441 cttcattgtc tttgagtctg agtagagcga gccgggaccg tggcatcgct cacaaccgat
     1501 tccctcggca cgctgtttct cggtcgtgaa actcggtgtt gtgacatgac acttgaggca
     1561 ctgcgccgac tgcgtgcagc gcgcctcgca cttgccgccg gcctcgccg
//
LOCUS       DAOQUC010001069         1608 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_103788_length_1608_cov_3.134578, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001069 DAOQUC010000000
VERSION     DAOQUC010001069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103788_length_1608_cov_3.134578"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..644)
                     /locus_tag="PKH07_15625"
     CDS             complement(<1..644)
                     /locus_tag="PKH07_15625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36416.1"
                     /translation="MKLGLKTRVYAGMNLRQSQSAFLAVCTQIIQKVSPSQVALRLGL
                     LPVDDEDSDRTESDDFILEDRDDDEQDSEDTDYGEISTATLCTPRSPSAVLDLNDIYA
                     IRVLVTNGRYKCIFPEPDWMKGLTDGPYESGATQIRELLRGIARWFQEHRQTFLEKTI
                     PENFVGQEGVRNFDRDDPLVTHRGFLSAIGVKDDDLDSMASALSRLLPHIWFVW"
     gene            complement(659..>1608)
                     /locus_tag="PKH07_15630"
     CDS             complement(659..>1608)
                     /locus_tag="PKH07_15630"
                     /inference="COORDINATES: protein motif:HMM:NF016859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36417.1"
                     /translation="PDTQGYDVKEINAILDQKTLESLKKIQSSAPAYGFAVLAVSGVR
                     GAGQARQSVTARGYLSPGFVGVGSGYRAEQFFFGRPLISGMKREEAFYASMNARSTTG
                     DKKLVTRQAGGLMRRLVLACWEWVVRASMKDDNGNCRYECESPIRTPAQCQWGRHQVI
                     CPTCYLCGDASLPGYEDLDGFPAGLIAAQSFGERGTQLAMRSYHTGQRDVSIGGIRTA
                     LGDPEYFMKMTQSDNFVRTLAGTVTYRDVHRRHLYLIWRVIHESGDKKLSRVFLEKRG
                     ILSGLVADDQWDFILSRIGNDWESDDSALSRIIRSVWIP"
BASE COUNT          394 a          447 c          363 g          404 t
ORIGIN      
        1 tcccaaacaa accatatatg agggagcaaa cgactcaacg cgcttgccat ggagtccaga
       61 tcgtcatctt tgacccctat cgctgaaaga aaacctctat gcgtgacaag cggatcatcc
      121 cgatcaaagt tgcgtacccc ctcctgtccg acaaagttct ctggaatcgt cttttcaaga
      181 aatgtctgtc tatgctcttg aaaccatcgt gcaattcccc ggagaagctc acggatctga
      241 gtcgcaccgg attcgtaggg tccatctgtc agtcccttca tccaatcagg ctctggaaaa
      301 atacatttgt agcgaccgtt ggtcactaac acacgaatgg cgtagatatc attcagatca
      361 aggacagcag atggtgaccg gggagtgcaa agagtagccg tcgagatctc gccgtagtcg
      421 gtgtcctccg aatcttgttc atcgtcatct ctatcttcca ggataaaatc atcgctttca
      481 gtgcgatcag aatcttcgtc atcaaccggc aagagaccga gtcgcaatgc aacttgactg
      541 ggggagactt tctggattat ctgtgtgcat actgccagaa acgccgactg actctgcctt
      601 agattcatcc ccgcatatac acgtgttttc agaccgagtt tcatagctat tctcacctct
      661 atggaatcca aacacttctg atgatgcgac tcaatgcgga gtcgtcagac tcccaatcgt
      721 tgcctatgcg actaaggatg aaatcccact ggtcatcggc aaccagtcca gacagaatac
      781 ctcgcttctc aagaaacacg cgtgacaact ttttgtctcc gctctcgtga atgactctcc
      841 atatcaggta caaatgtcgg cgatggacat ccctgtaggt tactgttcca gcaagtgtcc
      901 tgacaaaatt gtcggactgt gtcatcttca taaagtattc cggatctcca agagcggtgc
      961 gaatccctcc tattgagaca tcacgttgtc cggtgtggta tgaacgcatc gccagttgtg
     1021 ttcctctctc accgaaggac tgtgctgcga tgagtccagc gggaaacccg tccaagtcct
     1081 cgtatcccgg tagtgacgca tcgccacaca ggtaacaagt cggacagata acttggtgtc
     1141 tgccccattg gcattgagca ggcgttcgga tgggcgattc gcattcgtat cggcagttgc
     1201 cattgtcatc tttcatagat gctctgacca cccattccca acaagcgaga accagtcgtc
     1261 tcatcaatcc tcctgcctga cgtgtcacga gtttcttgtc accggttgta ctgcgtgcgt
     1321 tcatggaggc ataaaaggct tcttcccgct tcatcccaga tataaggggt ctcccgaaaa
     1381 agaactgctc tgccctatat cctgaaccaa ctccaacaaa cccagggctg agatagcctc
     1441 tcgccgtgac gctctgtctt gcttgccctg cgccacgcac gccgctaacc gcgagaacgg
     1501 caaaaccata ggcaggagca gatgactgaa tcttcttcag agattcaagt gtcttctgat
     1561 cgagaatggc gttgatctct ttgacatcat acccttgagt atctggca
//
LOCUS       DAOQUC010001070         1607 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_103918_length_1607_cov_2.377577, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001070 DAOQUC010000000
VERSION     DAOQUC010001070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1607
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103918_length_1607_cov_2.377577"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..779
                     /locus_tag="PKH07_15635"
     CDS             <1..779
                     /locus_tag="PKH07_15635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36418.1"
                     /translation="TRSDLIVLSERSHEFVVGKTVRCANYYLDPINDIDYYKVMYSDG
                     TFLVHDFSLRDNDFPWGMAYTGTGHDFTAAATLVAQDGTRHHVLAKGGFYTDETQPDN
                     GLIPTTDDTFDNQTNQNFTTTEISGEYRFNWDRLTSWKQRKPLAAVFFIGDGATSAHL
                     GSVSPISLKWWGDFQEVPGTPNTVAPVREVQMTTDWSYRCALLQSSRFFFKIGFTLAG
                     HSSDDSDFTYHRRPAVEGDLDKNFYGSIWEAAYLIGDENT"
     gene            854..1390
                     /locus_tag="PKH07_15640"
     CDS             854..1390
                     /locus_tag="PKH07_15640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36419.1"
                     /translation="MEETSELQWEPPIEGNPFSHILIRFAGEGAKPAVSLPRDSRRFL
                     IPRTAKVEVSTFNDISNIESDRVSISVNNGIWEDATSSTFGPILAWDLYYDLNDIDAN
                     LVITTTVTAARNRRLLVWVLQHASTLHGISFDSAVFGNSASKHCDAGMMLVMEFAGRF
                     GSSKWWPITLDLQIDPTA"
     gene            1387..>1607
                     /locus_tag="PKH07_15645"
     CDS             1387..>1607
                     /locus_tag="PKH07_15645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36420.1"
                     /translation="MKTLTLFFVSLIAYAQLQTPLTVLPGAGTAPGSIRLRERRSNGT
                     NYFALKASPSMASDVEIQAPPDYPATNGE"
BASE COUNT          377 a          463 c          420 g          347 t
ORIGIN      
        1 acacccgttc agacctgatc gtgttgtccg aacggtccca tgaattcgtt gtcggcaaga
       61 cagtccggtg cgccaactac tacttggacc cgattaacga cattgactac tacaaggtca
      121 tgtattcgga cggaaccttt ctcgttcacg acttttctct cagggacaac gatttcccat
      181 ggggcatggc atacactggc acagggcacg atttcaccgc cgctgccacg ctcgtcgcgc
      241 aggacggaac ccgccatcac gtcttggcta agggcggctt ctacacggat gagactcagc
      301 cagacaacgg gctcattcca acaaccgatg acactttcga caaccaaacg aatcaaaact
      361 tcaccaccac agaaataagc ggagagtacc ggttcaattg ggatcggctg acgagttgga
      421 agcagcgtaa gccattagcc gccgtgttct tcataggcga tggggcgacg agtgcgcacc
      481 ttggttcagt ctccccgatc tcccttaaat ggtggggaga tttccaggag gttccgggaa
      541 ctcccaacac agtagcgcca gttcgcgaag tacagatgac gacggactgg tcgtatcggt
      601 gcgcgttgct tcagtcgagc aggtttttct tcaagatcgg cttcacgctt gccggtcact
      661 cctcggacga ttcagacttt acgtatcatc ggcggccggc ggttgagggc gatctcgaca
      721 aaaacttcta cggctcaatc tgggaagccg cctatctcat cggggatgaa aacacgtgat
      781 cgaccctcgc cagtacgcga acaaccaacg cctaggcttt ggaagacctc cgagggtgcg
      841 tggtgttcgc tatgtggaag agacctcaga attgcaatgg gaaccgccga ttgaaggcaa
      901 cccgttctcc catatactga ttcgcttcgc tggcgagggt gcaaaaccag cggtatccct
      961 accgcgggat tctcgacgtt ttcttatccc acgcacggcg aaggtggagg tatccacctt
     1021 caacgacatt tccaatatcg aaagtgatcg ggtgtcgata tcggtcaaca acgggatatg
     1081 ggaggacgcc acaagttcca cattcgggcc gattctcgcg tgggacctct actacgatct
     1141 gaacgacatt gacgccaacc tggtcatcac caccacagtc acagcggcac gtaaccgtag
     1201 gcttctcgtg tgggtccttc agcacgcatc gaccctacac ggcatatcgt ttgacagcgc
     1261 cgttttcggc aattcggcca gcaaacactg cgatgccggt atgatgttag tgatggagtt
     1321 cgccggccgt ttcggatcat cgaaatggtg gccaatcact ttggatctac aaatcgaccc
     1381 aacagcatga aaacactcac gcttttcttt gtatcgctca tcgcatacgc tcaattgcaa
     1441 accccgctca cagtgctacc tggagcggga acggcacccg gtagtatccg gttgcgagag
     1501 cgccgttcga acggaaccaa ctacttcgcc ttgaaggcat ctccctccat ggcatctgac
     1561 gtggagatac aggcgccgcc ggactatccg gcgacgaacg gagaatg
//
LOCUS       DAOQUC010001071         1607 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_103919_length_1607_cov_2.355026, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001071 DAOQUC010000000
VERSION     DAOQUC010001071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1607
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103919_length_1607_cov_2.355026"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            33..>1607
                     /locus_tag="PKH07_15650"
     CDS             33..>1607
                     /locus_tag="PKH07_15650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36421.1"
                     /translation="MPRSEITEMFSVYVDESEANIQGTIPPIEDGPAEWASFEVFFLD
                     RGRGTIPNSNLQAFSEVLVFRRSVREWGPSDWDRARFNREDAILVTPVTSNPMLIDMR
                     TAGNDAVIYNQEADFVVAITTSSRAQNNDQFSIVLSNVFLSHFYPDPYLDKEATTKLI
                     TCVDSAGDLMPERDFITAAGSMDRKYKFFPPNYPDFAGQRHADGLDSLEDLEIPQVLP
                     SECTPTAVIGIEMAARRDRLRRVFLNFVSSMDGANSDNDYWLDDPVLPGDTLHHFGVY
                     DPHFDEIIQDNDNNGIFTPADTVLYPGANGRLDSKMTLFDDDMDGLVDEEELNGINDD
                     HDYWADENANSLYDPGVDEHYFDYDRDGLYSDATVLDERLDTDGTVTAGAGTMLDSIA
                     IGAALTPFQQADKVGYTVRDGNVFDENVDIVIFDMDGDGKYTFKPDRFVDSDGNDSIK
                     PGERNVPNGSPATAFRILDNVFADNLDDPIYVWLEVDYDDTYTSADSPILGRPAAGVT
                     VSGFNVADATWAFWDQN"
BASE COUNT          382 a          410 c          430 g          385 t
ORIGIN      
        1 tcaatgacct gaccatcgaa cgtcaaggaa tcatgccgcg cagtgagatc acggagatgt
       61 tctctgtgta cgtggatgaa agcgaagcca atattcaggg aaccatcccc cccatcgaag
      121 acggtcccgc tgaatgggcc tctttcgaag tattttttct ggatagaggg cgaggaacga
      181 ttccaaactc caaccttcag gctttcagtg aggtactggt cttccggcgc tcggtcaggg
      241 aatggggacc atccgattgg gaccgtgctc ggttcaaccg agaagacgcg attctcgtca
      301 ctcctgtgac atcgaaccca atgctcattg atatgcgaac tgcaggcaat gatgcggtta
      361 tttacaacca ggaagcggat ttcgtcgttg ccattacaac cagcagccgt gctcagaaca
      421 atgaccagtt ctctatcgtg ctgagcaacg tgttcctgtc gcacttctat ccggatcctt
      481 atctggataa ggaagctacg acgaaactga ttacctgtgt tgattcagct ggagacctta
      541 tgcccgagcg ggactttatc actgcggcgg gatccatgga tcgcaagtac aagttcttcc
      601 caccgaacta cccagacttc gctgggcaga gacatgcgga tggacttgat tcactggagg
      661 atttggagat tccccaggtg ctgccctcgg aatgcacacc aacagccgtg atcggaatcg
      721 agatggcggc acggcgagat cggcttcgcc gggtcttcct gaactttgtg tccagcatgg
      781 atggagccaa ctcggacaat gactactggc tcgatgatcc agtgcttcca ggtgacacac
      841 ttcatcactt tggcgtttac gatccccact ttgatgagat cattcaggac aatgacaaca
      901 acggtatctt cacgcctgcg gacaccgttc tttatccggg agccaatggt cgtttggaca
      961 gcaagatgac tctgtttgac gacgacatgg acggtctggt tgatgaggaa gaactcaacg
     1021 gaatcaatga tgaccatgac tactgggcgg atgaaaatgc caacagtctg tacgacccgg
     1081 gtgtggatga gcattatttt gactacgatc gcgatggtct ctacagcgat gcaacggtcc
     1141 tcgacgagcg gttggacacc gatggcaccg tgacagctgg tgccggaacg atgcttgata
     1201 gcattgccat tggcgctgcc ttgactccat ttcagcaggc tgacaaggtc ggatataccg
     1261 tacgggatgg aaatgtcttt gatgaaaacg tggacatcgt catcttcgat atggatggcg
     1321 atggaaagta cacgttcaag cctgaccggt tcgttgactc ggacggaaac gattcgatca
     1381 aacctggcga gcgcaacgtt ccaaacggct ctcccgcaac ggcattccgt attctcgaca
     1441 acgtgtttgc ggacaatctc gatgacccga tttatgtctg gttggaagtt gactatgacg
     1501 atacttatac ctccgcagac agccccattc ttggcagacc agctgcgggt gttaccgttt
     1561 ctggcttcaa tgttgcggat gcaacttggg ctttttggga ccagaac
//
LOCUS       DAOQUC010001072         1606 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_103967_length_1606_cov_3.900064, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001072 DAOQUC010000000
VERSION     DAOQUC010001072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1606)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1606)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1606
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103967_length_1606_cov_3.900064"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            709..1095
                     /locus_tag="PKH07_15655"
     CDS             709..1095
                     /locus_tag="PKH07_15655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36422.1"
                     /translation="MEVLHWGHNYETFSNCLKKHPDGKTLLIETEHLTGLAALQNALA
                     VHRAHLRLVVLAPLTSDELTLLLDLGLFLSGLAVIVVLPPGDGEMHNKVHRLHPRFVA
                     SGQNAWDDAVSVAVNILRKSESRVST"
     gene            1236..>1606
                     /locus_tag="PKH07_15660"
     CDS             1236..>1606
                     /locus_tag="PKH07_15660"
                     /inference="COORDINATES: protein motif:HMM:NF024140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCP four helix bundle domain-containing protein"
                     /protein_id="HNT36423.1"
                     /translation="MFKDMKVGTKIVLGFVLLILIAVALGGLAVVNMLSVKTTATDLA
                     HEKVPAVGLANNVERWSLNTMYEARGYGYTEETDYLDKARKNLAEVKKYLTDAKEHAK
                     KYDLKVLAENADKAEKAALEY"
BASE COUNT          418 a          357 c          456 g          375 t
ORIGIN      
        1 tgagatggga actgtcatgt tcatggcctc cttgcacctt tttcaggaca acacagcatt
       61 ccgtcaaaga gtaccgatgt caacctctcc aaaaggaacc cccggagatt gagtgaaacc
      121 taacagttcc agagcattcg gtcaaatggc ggcagggatg ccgagtatca cctcagttac
      181 acaatggcgg agcctggcac tccgaacgac tgaggcttgg ggaaaaagtg aggaatcttg
      241 cagatgcttc ggtagcaagg ctctgcgcgc tgccgaagca gggcgtgcga gtgacggggc
      301 ttcggcagcg ttgcaaccga agcatgcaga aaatcccccc tgccccttac cgaaggggga
      361 taagagcggt tctgcccccc ttaacaaggg gggatcaagg ggggattttt gggagagcat
      421 gcagaagcgt ctgtaagagt gggcatagcc cacacgcgtg caggtgctgg tctgcgcaga
      481 aggactcgtt ggagatagtc ttctttgtct tctgtctgat atttcagact gagaaatcag
      541 acatttcgag ggcaactcct ttcctctgtc atttccctat ccgacacaac atattgagtg
      601 gcaatgagtt aagtgaatct gcacgcctca ctacagagtt ggcctgtcta ttgctgtatg
      661 tatttccatc tcgtttcgat tttacaggca acttcgggag agagttcaat ggaagttctt
      721 cactggggac acaactatga gactttctca aattgtctga agaagcatcc tgatggcaag
      781 actctgctca ttgaaacgga gcatctgacc ggccttgctg cacttcagaa tgctcttgca
      841 gtgcatcgag cacacttgcg tcttgtagtg ctggcaccgc tgacgtcgga tgaactgact
      901 ttactgttgg atctgggtct ctttctcagt ggtcttgcag tcatcgtcgt tcttccgcca
      961 ggggatggtg agatgcacaa taaagtacat cggctccacc cgaggtttgt cgcatcagga
     1021 caaaacgctt gggacgatgc ggtctctgtt gcggtgaaca ttttgagaaa atccgagagc
     1081 cgtgtatcaa cctagaggga agcgagccgc tccaaagggt ttgaggagtt cattcctgga
     1141 aataacgaag ggaggtagga ggcagggaag agataaggtt gggagcacag tggaagactt
     1201 ggtttgaatg cttaaggaat aaggagtcga agaaaatgtt caaagatatg aaagtgggaa
     1261 cgaaaatcgt attgggattt gtattgctaa tcttgattgc ggtcgcattg ggtggtcttg
     1321 cggttgtcaa tatgctgtct gtcaagacga ctgccaccga cctggcccat gagaaagtgc
     1381 ctgctgtcgg tctggcgaac aacgtagaac gctggtctct taacacgatg tacgaggcca
     1441 gaggatatgg gtacacggag gaaacagatt atctcgataa ggcacggaag aacctcgcag
     1501 aagtgaagaa atacctgacc gacgccaagg agcatgcaaa gaagtacgat ctcaaagtgc
     1561 ttgctgagaa cgccgacaaa gcggagaaag cagctctgga atatga
//
LOCUS       DAOQUC010001073         1606 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_104025_length_1606_cov_2.530625, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001073 DAOQUC010000000
VERSION     DAOQUC010001073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1606)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1606)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1606
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104025_length_1606_cov_2.530625"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            214..828
                     /gene="coaE"
                     /locus_tag="PKH07_15665"
     CDS             214..828
                     /gene="coaE"
                     /locus_tag="PKH07_15665"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HNT36424.1"
                     /translation="MFVVGLTGGLGAGKSTLASFLVQQGATVIDADSLAHRFLEPGTS
                     QYRALLETFGENILTETGAVDRARLADRAFASPETVKMLNGIIHPALVAEIRRRIAEA
                     REVKKGVLVVDAALLLEWDLDDLFDVIVNVEVAGDIGLLRAGKRMAQSADRLRGRLQA
                     QLSAEERRRRSSLTIQNNLDKADLEKKARELWNRWTERPSESEA"
     gene            834..>1606
                     /gene="rho"
                     /locus_tag="PKH07_15670"
     CDS             834..>1606
                     /gene="rho"
                     /locus_tag="PKH07_15670"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012645774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HNT36425.1"
                     /translation="MEIAELKQKTVAELTKLAKKLKVEDYSGMRKQELIFNILKTQSE
                     TDGFMFGEGVLETLPDGFGFLRSPTYNYLPGPDDIYVSPSQIRRFGLRKGDIVTGQIR
                     PPKEGERYFALLKVQAINYENPEHARDVVLFDNLTPLYPNERFKLETRPESITSRIID
                     LITPIGKGQRGLIVAPPRSGKTVILQEIANSITANHPNVILIVLLIDERPEEVTDMER
                     SVKGEVVSSTFDEPPERHVQVAEMVLDKAKRLVEYKKDVV"
BASE COUNT          395 a          368 c          508 g          335 t
ORIGIN      
        1 ccgaagagga gcgcgaacgt ttgggtgctc tggtggtgga tggaatggag aaggcgatgg
       61 cgctgtctgt gccagtcaag gtggatctga agattggagc caactgggcg gaaatctgat
      121 ggatatgagg aacccgaagt ttacaggtgt tgtgcgtatt gaagagcctt gtgttcgttt
      181 cggccaacac cggggccagc gagaggacag attgtgttcg tagtgggtct gacaggtggg
      241 cttggagccg gaaagtcaac gctggcgtca tttctggtgc aacagggagc gacggtcatt
      301 gacgcggata gtctggcaca tcggttcctt gagccgggaa ccagtcagta tcgagcattg
      361 ctggagacct ttggagagaa catcctgact gaaacgggtg cggttgatcg ggctcgattg
      421 gcagatcggg catttgcttc gcccgagacg gtcaaaatgc tgaacgggat catccatcct
      481 gccctggtgg cagagattcg acgcaggatc gcggaagccc gtgaggtcaa aaaaggcgtt
      541 ctggtagtgg atgcggcgtt gcttctcgaa tgggatcttg atgatctctt cgatgtgata
      601 gtgaatgtgg aagtggcggg agatatcggt ttgttgcgcg ccggcaagcg aatggcacaa
      661 agcgccgacc gcttgcgcgg caggctgcaa gcccaactct cggctgagga aagaagaagg
      721 aggtccagtc tgacgattca gaacaatttg gataaagcgg atttggagaa gaaggcacgg
      781 gaattatgga accgatggac agaaagacca tcagaaagtg aggcatgaga aaagtggaga
      841 ttgcagagtt aaaacaaaag acggttgccg agctgacgaa gctggcaaag aagctcaagg
      901 tcgaggatta cagcggcatg aggaagcagg agttgatatt caacatcctg aagacgcagt
      961 ccgagaccga tggtttcatg tttggagaag gagtgttgga gacgcttccg gacggttttg
     1021 gattccttcg ttcgcccacc tacaactacc ttcctggtcc cgatgacatc tacgtttcgc
     1081 catctcagat tcggcgtttt gggcttcgca agggagacat tgtcaccgga cagatccgtc
     1141 ctccaaagga aggggagcgg tatttcgcgc tgctgaaggt tcaggcaatc aactatgaga
     1201 atcccgagca tgccagggat gtcgttctgt tcgacaacct gaccccactg tatcccaacg
     1261 agcgcttcaa actggagacc cgaccggaga gcatcacctc gcgaatcatt gatctgatca
     1321 ccccgattgg aaagggacag cgcggtctga tcgtcgcccc gccccgatcg ggtaagacgg
     1381 tcatccttca ggagatcgcc aacagcatta cggccaacca tcccaatgtc atccttatcg
     1441 tactgctgat agacgagcgg cctgaggaag tcaccgacat ggagcgaagc gtcaagggtg
     1501 aagtggtcag ttccaccttc gatgagccac ccgagcgtca cgttcaggtt gccgagatgg
     1561 ttctggacaa ggccaagaga ctggtggagt acaagaagga cgtcgt
//
LOCUS       DAOQUC010001074         1605 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_104106_length_1605_cov_3.129032, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001074 DAOQUC010000000
VERSION     DAOQUC010001074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1605
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104106_length_1605_cov_3.129032"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            167..>1605
                     /locus_tag="PKH07_15675"
     CDS             167..>1605
                     /locus_tag="PKH07_15675"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HNT36426.1"
                     /translation="MDKSRSSGLLFYLCILLYGALGIALQVFLLRRLLATLGGNELSV
                     VFGLAIWLLGAGLGSWISGNRSRFHKHNSTLCFLSLSAAFLFPICSLWSPFAVRGLTG
                     HVIGQAYTPSEMLLCSVVLIGLPCFLWGCLFPVLCRIGNAFGVQSSRVFLFEAVGAGI
                     GGLLYTFLFLPSFDEWTIALVLVAASVCQWIAMVWVVPGWRHRGSLSAGILGVALLAC
                     LMGSVGQLRNLALDANWQGFDVLEAKDSLYGSLVVTQQESQISFHENGTVLFSYPDEE
                     SYEDVIHIPLLQHPNPKTLLLVGGGPAAITEAGQHPLDEIHYVELDSSLVSLIKRYAA
                     GHEQSVLDDPRVTLSRADARREIKNARQRFDVIAVCLGDPTTLQTNRLFTEEFFSEAR
                     RCLAPGGILAFEVSSAENVLSDDLVRYLQLIRDTLSSVWEQVLCVPGLHGLFLASDQA
                     TGFETDVSRLYERHEQRAIQSQYLFYTHLP"
BASE COUNT          311 a          399 c          430 g          465 t
ORIGIN      
        1 taaaacataa tacagagtcg aggttctatc tgtcaaatga tttgaccgtg caccaagtcg
       61 gcatggcgag ataggtgcga atcttctcat ctgtgttgtc aagagatgac tccagttcta
      121 cactcaacta agttcgaaga cattcattgc ggtgcgttct atggagatgg acaagtctcg
      181 ctcatctgga ctgttgtttt atctgtgtat tctgttgtat ggagcattgg ggatagcact
      241 ccaggtcttc ctgctgcgaa gactgcttgc gacgcttggt ggcaatgaac tcagtgtggt
      301 attcggcctg gcgatctggc tgttgggggc tggactgggg agttggattt caggcaacag
      361 gagcaggttc cacaagcata attccacttt gtgctttctc tccttatcgg ctgccttcct
      421 gttccccatt tgctccctgt ggagtccctt tgcggttcgt ggtttgaccg gtcatgtgat
      481 aggccaggct tacactcctt ctgaaatgct gctctgttca gtggttctga ttggactgcc
      541 ctgctttctt tggggttgtc tttttccagt gctctgtcga atcggaaatg cctttggcgt
      601 tcaaagcagc cgggtttttc tgtttgaggc ggtcggtgct ggaatcggtg ggttgctcta
      661 cacctttctt tttctcccct cctttgacga gtggacgatt gcgctcgtgc tcgtagcagc
      721 atcggtctgc cagtggatcg ccatggtatg ggtggtacct ggatggcgtc atcgaggttc
      781 tctctctgct ggtatccttg gagttgctct gctggcttgc ctgatgggct ctgtcggaca
      841 gctcagaaat ctggctctcg atgcaaattg gcagggcttt gacgtgctcg aagccaagga
      901 cagtctgtac ggttcgttgg ttgttacaca gcaggaatct caaatatcct ttcatgagaa
      961 cggcactgtt cttttctcct atcccgatga ggagtcctac gaggatgtga ttcatattcc
     1021 acttctccaa cacccgaatc cgaaaacgct gcttttggta ggtggtggcc ctgctgccat
     1081 cactgaagcc ggccaacatc cactggacga gattcactat gtggaattgg attcgagtct
     1141 ggtttcattg atcaaacgtt acgctgctgg acacgagcag tcggtgctgg acgatccacg
     1201 ggttaccctt tcacgtgctg atgccaggag agagatcaag aacgcaagac aacgctttga
     1261 tgtcatcgct gtttgccttg gcgatccgac cacactgcag accaacaggc ttttcaccga
     1321 ggagttcttc tctgaagccc gacgatgtct tgcacctggg ggaattcttg cgttcgaagt
     1381 ctcatccgct gagaacgtcc tttctgatga tcttgtgagg tatctgcaac tgatccgaga
     1441 tacactctcg tccgtctggg aacaggtgct ttgcgttcca ggactgcacg gcctgttcct
     1501 ggcatcggat caggcaaccg gttttgaaac cgacgtgagc aggctttacg aaagacatga
     1561 gcagcgcgct atccaatcgc aatacctgtt ttacacgcac ctgcc
//
LOCUS       DAOQUC010001075         1605 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_104107_length_1605_cov_3.128387, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001075 DAOQUC010000000
VERSION     DAOQUC010001075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1605
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104107_length_1605_cov_3.128387"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1469)
                     /gene="fliD"
                     /locus_tag="PKH07_15680"
     CDS             complement(<1..1469)
                     /gene="fliD"
                     /locus_tag="PKH07_15680"
                     /inference="COORDINATES: protein motif:HMM:NF018852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HNT36427.1"
                     /translation="MAATQNGQDQTFFFGRKSSDFVAARETLITLQSAFGRLNNLLRQ
                     TLESKSLLGLSARSSEQAIRMSVNAGSKTGTTVFQLQSTAQTYRISSDVFGDPDSPLD
                     FTGALSIGGVRVTISPSDTLNSLAASLNQTEDVNGNSLLEDSEDRNRNGLLDFGNQNS
                     GVSAWVEDGRLFLESITAGSSPFSINSENGVAESLGLLKVVPNNTTHYKNIVHRGTDA
                     VIAHEGQIYVGDGNRFTNLPNTISLEINPQTLPFAPSEANPLAFTATVSASPENSARA
                     IREFVSTYNKVMAEFNDAVMYPGVLDSDVLLFTLRADMLEALNRKQAPNFSNDTPLQQ
                     IADMGIEMRSSQRLRIPQLALIQMRNRRMAGVDQKSAEAVDSVPGFLKSLQQIGVVDR
                     TDGLLEIDETTLHKALVENREQVYSLLASEDQGVLEAFRRLVAEASSQKSGRVNNQIQ
                     FYDNMVSRHERIPVERQRYFEFSAEQFTARKAMGELLDVR"
BASE COUNT          353 a          418 c          444 g          390 t
ORIGIN      
        1 cggacatcca ggagttcacc cattgccttt cgagcggtga actgctctgc cgagaactcg
       61 aagtagcgct ggcgttcgac gggaatgcgt tcatggcgtg aaaccatgtt gtcatagaac
      121 tgaatctgat tgttgactcg tccggacttc tgcgaagagg cttctgccac cagccttctg
      181 aacgcctcca ggactccttg atcctctgaa gccagaaggg aatagacctg ttctctattc
      241 tcaacaagtg ccttgtgaag agttgtctca tcgatctcca gcaggccatc tgttctgtcc
      301 acgactccga tctgctgcag gctcttcaga aatccgggta ccgaatcaac agcttccgca
      361 gatttctgat ccactcctgc catgcgcctg tttcgcatct ggataagcgc tagttgcgga
      421 atacgcaggc gttgtgaact gcgcatttcg attcccatgt ctgcaatctg ttgcaggggt
      481 gtgtcgttgg agaagttcgg tgcctgcttt cggttgagtg cctccaacat atcggcgcgc
      541 agagtaaaaa gaagcacgtc ggagtctaag acaccgggat acatgaccgc gtcattgaac
      601 tcggccatga ctttgttgta ggtgctcaca aattcccgaa tagccctagc agagttctcc
      661 ggacttgccg aaacagttgc ggtgaacgca aggggattgg cctcggacgg agcgaaggga
      721 agtgtctgtg gattgatctc caatgagatt gtgttgggaa gattggtgaa gcgattcccg
      781 tctccgacat agatctgccc ttcgtgagcg atcacggcgt ccgttccccg gtgcactatg
      841 ttcttgtagt gcgtcgtatt gttggggacg actttcagaa gccctagcga ttcagcgaca
      901 ccgttctcac tgtttatgga gaagggagac gacccggcag ttatagactc caagaacaga
      961 cgaccatcct cgacccaggc ggacacaccg gaattctgat ttccgaagtc caacaagcca
     1021 tttcgattcc gatcctcgct gtcctcaaga agtgaattgc cattgacatc ctcggtttga
     1081 ttgagcgagg cagccagcga gttgagtgtg tccgacgggg aaatggtcac acgtactccg
     1141 ccgatggaca gagcacctgt gaagtcgaga gggctgtctg ggtcgccgaa aacatcactc
     1201 gagatccggt aagtctgtgc ggtggattgg agttgaaaga ccgtggtgcc cgtcttgctg
     1261 cctgcattga ccgacatgcg gatcgcttgt tccgaggaac gagcagacag tccgaggagg
     1321 gacttactct ccaaagtctg cctcagcagg ttgttcaacc ttccgaaagc agactgaagt
     1381 gtgatgagcg tttctcgggc ggccacaaag tctgaggatt tgcgtccaaa gaagaaggtt
     1441 tgatcttgtc cgttctgtgt cgctgccaac aggtctctca atgctgaacg ggttacttgt
     1501 gccgagccca ttccgtcgaa gatttccagg acagggccga cctccctgtc cagacctaga
     1561 aaccatttcg agcctatctg tcgcatggat cggctgtgaa tctgg
//
LOCUS       DAOQUC010001076         1605 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_104110_length_1605_cov_3.050968, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001076 DAOQUC010000000
VERSION     DAOQUC010001076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1605
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104110_length_1605_cov_3.050968"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..582
                     /locus_tag="PKH07_15685"
     CDS             <1..582
                     /locus_tag="PKH07_15685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HNT36428.1"
                     /translation="INGDGRGDFVAPDGWLEAPENPWEGEWTWHPEFQLGSASVPILV
                     HDVDDDGLADLIVGGAHSYGLNWWKQTISSEGSRTWSRHEIDPNRSQYHDMILCDLDN
                     DGTLELITGKRYRAHNGMDPGANDPVGVYYFESDGGAFKRATLDYGHASSSSGVGIAF
                     WVEDVDANGWKDIIAPGKEGLYLFLNTGRRASE"
     gene            complement(593..1411)
                     /gene="dapF"
                     /locus_tag="PKH07_15690"
     CDS             complement(593..1411)
                     /gene="dapF"
                     /locus_tag="PKH07_15690"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HNT36429.1"
                     /translation="MNSRVLNFTKMSGLGNDFIVFDNRDGAIPPSEYPAIAKRLCPRR
                     ISVGADGILVLENHPEFDFRMHYLNPDGTEAMCGNGARCLVRFAHLLGVISDETVFLA
                     DDGAHRAFVRPEVIRLELTSPRDVRLNIALDLGGLGNFISHFVNVGVPHAVIFVNDLQ
                     AVDVNRVGRMIRYHQEFAPAGTNVNFVMVKDRGNIEVRTYERGVEDETLACGTGSTAS
                     ALVAGLLDLAEPPVHIFTRSGGSLSVDYTRDADAIRDVFLTGDATVVYKGEVSI"
     gene            1410..>1605
                     /locus_tag="PKH07_15695"
     CDS             1410..>1605
                     /locus_tag="PKH07_15695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36430.1"
                     /translation="MVLRRSIVVNRIVASVPQDGAETPAFHTLTGRQPDGSEQSCRLE
                     PLISDEHVAGRCRMDTVGCPD"
BASE COUNT          405 a          426 c          434 g          340 t
ORIGIN      
        1 atcaacggag atggacgtgg agattttgtc gcaccggatg gttggctgga ggcaccagaa
       61 aatccttggg aaggggaatg gacgtggcat ccggagtttc aacttggctc tgccagtgtg
      121 cccattctcg tccatgatgt ggacgatgac ggtctggccg atctgattgt cggtggcgct
      181 cacagttacg gcctgaactg gtggaagcag accatctcct cggaaggcag ccgcacgtgg
      241 tcacgccatg agatcgaccc gaaccgctct cagtatcatg acatgattct ttgtgacttg
      301 gacaacgatg gaacactcga actcatcacc ggcaagagat accgggcaca caacggcatg
      361 gatcctggcg ccaacgatcc ggtcggcgtc tactatttcg aaagcgatgg cggtgccttc
      421 aaacgcgcga ctctggacta cggccatgcg tccagcagca gcggagtcgg aatcgccttc
      481 tgggtggaag atgtagatgc caatggctgg aaggatatca tcgctcctgg caaggaaggg
      541 ctttatctgt ttctgaacac ggggagaaga gcaagcgagt agcagaggca gatcagatgc
      601 tcacttcccc tttgtagaca acggtggcat caccagtgag aaacacgtct ctgattgcgt
      661 ccgcatcacg ggtgtagtca actgaaagcg acccgccact tctcgtgaaa atatgcactg
      721 gtggttcagc gagatcgaga agacctgcga ccagggcact ggccgttgat ccagttccgc
      781 aagccaaagt ctcatcttca acgccccgtt cataggttcg aacttcgata ttgcctctgt
      841 ccttaaccat aacgaaattg acattcgttc ccgcgggagc aaactcctga tggtagcgaa
      901 tcatccttcc gactcgattc acgtcaaccg cttgcaggtc gttgacaaag atgactgcgt
      961 gaggaacccc aacattcacg aaatgggata tgaagttgcc caatccaccc aagtctaagg
     1021 caatattaag tcgaacgtct ctgggagatg tgagttcaag gcgaatgacc tcaggacgga
     1081 caaaagcccg atgagcacca tcgtcagcaa gaaaaaccgt ctcatcggag atgacaccca
     1141 acaaatgagc aaaccgaacc aggcatcggg ctccattgcc acacatggct tccgttccat
     1201 ccggattgag atagtgcatt cggaaatcaa attccggatg gttctccaac accagaatcc
     1261 catctgcacc gacactgatg cggcggggac agagtctctt cgcaattgca ggatactctg
     1321 aagggggaat cgctccgtca cgattgtcaa aaacaatgaa atcgttgccc aagcccgaca
     1381 ttttggtgaa attgagaacg cgactgttca tggtgttgcg acgctccatt gttgtgaatc
     1441 gaatcgtggc atcagtccca caagatggcg ctgaaacccc ggctttccat actctcacag
     1501 gcaggcagcc ggatggttca gagcagtcct gtcgccttga gccactgata agtgatgagc
     1561 atgtggccgg ccgatgtcgg atggacaccg tcggatgtcc agacc
//
LOCUS       DAOQUC010001077         1604 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_104193_length_1604_cov_3.910910, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001077 DAOQUC010000000
VERSION     DAOQUC010001077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1604
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104193_length_1604_cov_3.910910"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1333
                     /locus_tag="PKH07_15700"
     CDS             <1..1333
                     /locus_tag="PKH07_15700"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNT36431.1"
                     /translation="LGLGSLMALTRQAGHEVFFIDNYLQCSDFIQQRFLQENRVDCIG
                     IYANTICYRDTLRMFSEIEELREQGAWRGKMLVGGPHTSVALETIPEFVDHVVQGEGE
                     HALMDILQGRETERVIPGRRVDDLDSLPFQPWDVFAALPYDFTCPWIDASHVFTLNTS
                     RGCPFNCAFCSVGSIWGNRYVCQSPERVVSEMQMLMNDYGAQGFYFREDHFTLSLKRT
                     TSFCELLLSKNINTQWACETRVDRMTEELVRLMARAGCKAFYLGVESGSERILALLNK
                     QITLAQIENTIRWCKKYGIKTYCSLIVGVPGETYDDFLQSEALMQRLRPHSYSFNVFV
                     GIPKSPLYLQCLKDKSYEYMDDLGLLYLPGFDIKCGYFYGLDSSVLVDHKFRQRTAFD
                     RQLNARWRRRKIYWTVAGMIPPRLRQALRPLKNRVAKVLGSVSRPGQNNED"
     gene            1345..>1604
                     /locus_tag="PKH07_15705"
     CDS             1345..>1604
                     /locus_tag="PKH07_15705"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNT36432.1"
                     /translation="MRTENVAPSISVLMSVYNAMPYLPQAVESILGQTFGDFEFIVIN
                     DASTDESREVIDSYRDARIRLLDNKENLGLTRSLNRAFQHAQG"
BASE COUNT          371 a          393 c          439 g          401 t
ORIGIN      
        1 gttgggcttg ggaagcctca tggctttgac gcgacaggct ggtcatgaag tcttcttcat
       61 tgataactat ctccaatgca gcgacttcat ccagcagcgc tttctgcagg agaatcgggt
      121 tgactgtatt gggatatacg caaacacaat ctgctatcga gacacactga ggatgttcag
      181 tgaaattgag gaactacgcg agcagggtgc ctggcgtggc aagatgcttg tcggagggcc
      241 tcatacatcc gtcgctctgg agacaattcc cgagttcgta gaccacgtgg tgcagggaga
      301 gggagaacac gctctcatgg acatcctgca aggaagagag accgagcgag tcataccggg
      361 gcgccgcgtg gatgatctgg attcgcttcc gtttcagccg tgggatgtgt ttgccgcctt
      421 gccttacgac tttacttgtc cctggatcga tgcttcccac gttttcacgt tgaacaccag
      481 cagaggttgt ccattcaact gcgccttctg ttcggttgga tcaatctggg gcaatcgata
      541 tgtctgccaa agcccggagc gtgttgtgtc tgaaatgcaa atgctgatga acgactacgg
      601 ggctcagggc ttctattttc gggaggatca ttttacgctg agcctgaagc ggacgacttc
      661 attctgtgaa ctccttctct ctaaaaacat taacacccaa tgggcgtgtg agacgcgcgt
      721 ggaccgaatg actgaagagc tggtacgact catggcaagg gcagggtgca aagccttcta
      781 ccttggtgtc gagagcggca gcgaacgaat tctcgccctg ctcaacaaac agatcacact
      841 ggcgcagatt gagaacacaa ttcgttggtg taagaagtac gggattaaga cttactgcag
      901 cctcatcgtg ggcgtccctg gcgagaccta tgacgatttt cttcaatcgg aagccctgat
      961 gcagcgcctc agaccgcatt cctactcgtt caacgttttt gttggtattc caaagagtcc
     1021 gctgtacttg cagtgtctca aagacaagag ctatgagtat atggatgatc ttgggcttct
     1081 gtatcttcca ggctttgaca tcaaatgtgg ctacttctac ggtctggaca gctctgttct
     1141 ggtagatcac aaattccgtc agaggactgc ctttgaccgg cagctgaatg cacgatggcg
     1201 acgccgaaag atctattgga ctgttgctgg gatgatcccc ccgcgtcttc gtcaggcact
     1261 gcggcctctc aagaacagag tcgccaaggt acttggcagt gtttcccgac cgggtcaaaa
     1321 taatgaggat tgagtggtgt gggtatgaga actgagaatg ttgccccttc gatatctgtt
     1381 ctcatgtccg tgtacaatgc gatgccctac ctgccacaag cagtggagag tattcttggg
     1441 cagacgtttg gtgacttcga gttcatcgtc attaacgacg cttcgacaga tgaatcccga
     1501 gaggtcattg actcctatcg cgacgcaagg attcggctgt tggacaacaa ggagaatctt
     1561 ggtttgacgc gatcactgaa ccgagccttc caacacgcac aagg
//
LOCUS       DAOQUC010001078         1603 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_104294_length_1603_cov_4.051680, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001078 DAOQUC010000000
VERSION     DAOQUC010001078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104294_length_1603_cov_4.051680"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1603)
                     /locus_tag="PKH07_15710"
     CDS             complement(<1..>1603)
                     /locus_tag="PKH07_15710"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNT36433.1"
                     /translation="SRTVREKIIQVGKDLLTTEGESLGDLSRDDVTGIVLQSCCDLAQ
                     DYQRSGLHGKAAQILSLGREMVPDEPRLLEQMGDLYAEAGSIDRLAEVAEQLASLYRK
                     RGEIENIGDAYERLLSVDPGNLLGLERLVEWEQERGSLIGRFQMALSFANALSSKGLV
                     GRAAEILRQARSYDMDALSNLSDDDREAALQRILQATEKLASLNKARGEIREITLDRN
                     LLAGQLAAQQCHEQAIAIASEVVQDAPQESAAWLVLASSYHATGQLERALESYTRLAE
                     FFDTEATLTDRDSIRRAEDAYRTILALDPQALWAEENLARLLSRSGREREALDLLQAL
                     LEKIRKTKDVGRVISVARLIVSLDPNDRDALSVLKDTLTERGDTAGALGVLDSLVAIL
                     RRESSEKGKPSADVLDLLKQRIQLQPGRVEYRTELADFLAQTGEVEKATAEWSAVAEI
                     FISQGRADLAEKAFEKQIGLSPQSPRIREAFGGMLRSVGRLQDSAANYAIAARLYHEQ
                     NHVEESLKDVRTALQIDPWSEEAMQA"
BASE COUNT          342 a          479 c          419 g          363 t
ORIGIN      
        1 ctgcctgcat tgcttcttca gaccagggat cgatctgaag cgcggttcgg acatccttca
       61 gagactcctc gacatgattc tgttcatgat atagtctcgc agcaatagcg tagtttgcag
      121 cgctgtcttg aagacgtccc acagagcgca gcattccccc aaatgcttca cgaattctgg
      181 gcgattgtgg agaaaggcca atctgtttct cgaacgcttt ttcagcaaga tccgccctgc
      241 cttgcgatat aaagatctcg gcgactgcac tccattccgc cgtggccttc tcgacttcac
      301 ctgtctgagc cagaaagtcg gccaactcgg ttcggtattc gacccgacca ggctgcaact
      361 ggattctctg cttcagcaga tcgagtacat cggcagacgg tttccccttt tcagaggatt
      421 cacgccgcag aatcgccaca aggctgtcca acactccgag ggctccagca gtatcccccc
      481 gttccgtcaa agtgtccttc aggacagaga gcgcatcgcg atcgttggga tccagagaga
      541 ctataagccg tgcaacagag ataacacggc cgacatcttt ggtctttctg atcttttcga
      601 gcagggcttg gagaagatcg agcgcctcgc gttctctgcc cgatcgacta agaaggcggg
      661 caaggttctc ttcagcccac aaagcctgtg gatccaaagc cagaatcgtt cgataggcgt
      721 cctctgcccg tcgaatgcta tccctgtcgg tcagcgtggc ttcggtgtca aagaactccg
      781 cgagtctagt gtagctttca agggctcgct ccaattggcc tgtcgcatga tagctggagg
      841 caagcaccag ccaggcggct gactcctgcg gtgcatcctg gacaacctct gaagcgatgg
      901 cgatcgcctg ctcatggcat tgctgtgcag ccaattgacc agcgagcagg ttcctgtcca
      961 gtgtgatctc tcgtatctcg ccccgagcct tattcagaga ggcaagtttt tcggttgctt
     1021 gaaggatgcg ctgaagcgcc gcttcccgat catcgtcgga gagatttgac agggcgtcca
     1081 tgtcgtacga acgcgcctgc ctcagtatct cggcagcgcg tccgacaagc cccttggaac
     1141 tgagcgcatt ggcaaaggac aaggccatct ggaatcggcc gatcagagag cctcgttcct
     1201 gctcccattc aacaagccgc tccaatccaa gaagattccc tgggtcaaca ctcagaagcc
     1261 gctcgtaggc atcgccgata ttctcaatct caccccgctt gcggtacagt gaagcgagtt
     1321 gctccgcgac ttcggcaaga cgatctatgg aaccggcctc ggcatagagg tcgcccatct
     1381 gttccagaag tctcggctcg tcaggcacca tctcccttcc caaggagagt atctgagcgg
     1441 ccttgccatg caggcctgag cgctgatagt cctgcgccag atcgcaacag gactgcagca
     1501 cgatgccagt aacgtcatct cgggacagat cgccaaggga ttcgccctct gtggtcaaaa
     1561 ggtctttgcc gacttggatg atcttctctc gaacagttcg act
//
LOCUS       DAOQUC010001079         1601 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_104515_length_1601_cov_3.743208, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001079 DAOQUC010000000
VERSION     DAOQUC010001079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104515_length_1601_cov_3.743208"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /locus_tag="PKH07_15715"
     CDS             complement(<1..273)
                     /locus_tag="PKH07_15715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HNT36434.1"
                     /translation="MRITFCCPFVPMEWIAAHGIEANRIIPELSDRASIFELEGLCPY
                     VRCFLSDVLSDAEARPVLFTTTCDQMRRAPEILRMLSSEIPVALMHV"
     gene            complement(351..1154)
                     /locus_tag="PKH07_15720"
     CDS             complement(351..1154)
                     /locus_tag="PKH07_15720"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNT36435.1"
                     /translation="MKTINKIGLSDVQAVYSGPEGDLWELIMGQQIHIGGLTSSMDLS
                     EQAGIGSGMSGVDLCCCNGAGMRFLLRFRNVDRMTGVDATEKVIERGRWRCAEEGLSD
                     RINFVQADVCKTGLVSASVDFVWGEDAWCYVSDKAALVREASRLVRPGGIIAFTDWIE
                     GPQSLSAEEAERFMRFMKFPSVQDKFGYEALLKDVGCQILVSDYTGRFAPYIELYLNM
                     LEKQLTYDALKILAFNQELANGLLDEMRFAKQLAESGKLDQGLFVARRL"
     gene            complement(1196..>1601)
                     /locus_tag="PKH07_15725"
     CDS             complement(1196..>1601)
                     /locus_tag="PKH07_15725"
                     /inference="COORDINATES: protein motif:HMM:NF022677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3034 family protein"
                     /protein_id="HNT36436.1"
                     /translation="SIGIKDNDGLDYTLYASKMLSLCGRPLMLNAGLRSTEAAHIGLL
                     GFTDERRIVAEGSVCFMPLDRVVLAAEYRQKPNEYDRIPGLVEDEDDWWTLCAAYIFN
                     DHLSAAVGYAHFGNVLNHEANRSVGASIKYEF"
BASE COUNT          392 a          487 c          361 g          361 t
ORIGIN      
        1 cacatgcatc agagcaacgg gaatctccga cgacagcatc cgaagaatct ccggtgcacg
       61 gcgcatctga tcgcacgttg tggtaaacaa caccggtctc gcttcagcat cactcagaac
      121 atccgagaga aaacatcgaa cgtaagggca gagaccttcc aactcaaaaa tggacgctct
      181 gtcactcagt tcgggaatga tcctgttcgc ctcaattcca tgagctgcga tccactccat
      241 cggtacgaat gggcaacaga aagtgatcct catgcgttcc tacttctgaa aggacacgga
      301 cgctccctcc tcatcgaggg caacgggagc cgaatataac tgtcttatct tcacaagcgc
      361 cgcgcgacaa agagcccctg atccagtttg ccggactcag ccagttgctt cgcgaaacgc
      421 atctcgtcaa gcaatccgtt ggcgagttcc tggttgaacg ccagaatttt gagcgcatcg
      481 taggtcagct gcttctccag catattcaaa tacagttcaa tgtagggtgc aaaacgccct
      541 gtgtaatcgg agacaagaat ctgacagccg acatccttca gcagcgcctc atatccgaac
      601 ttgtcctgga cacttggaaa cttcatgaac ctcatgaacc gttccgcttc ctcggcagag
      661 agtgactgcg gtccttcaat ccagtctgtg aaagcgatga taccacctgg acgtaccaaa
      721 cgggatgcct cacgtaccaa tgcagccttg tccgatacat agcaccaggc gtcttcaccc
      781 cagacaaagt ccaccgatgc tgagacaagt ccagtcttgc acacatctgc ctgcacgaag
      841 ttgatccgat ccgatagacc ttcctcagcg caacgccatc ggcctctttc aatgactttc
      901 tccgtggcat ccacccctgt catccgatcc acgtttcgaa accgaaggag aaaacgcatt
      961 cccgctccat tgcagcaaca gagatccact ccggacatgc ccgaccctat ccctgcttgc
     1021 tccgacaaat ccattgaaga ggtcagcccg ccgatgtgaa tctgctgacc catgatcagc
     1081 tcccacagat cgccctcagg cccactgtac acggcctgaa cgtcactcaa tccaatcttg
     1141 tttattgttt tcatctcaat actcctggag atctcttttt cgcggtgtct tctcgctaga
     1201 actcatactt gatggaggcg ccgacggatc ggtttgcctc gtgattcagc acgttgccaa
     1261 aatgagcgta gccgacagcg gcactcaggt gatcgttgaa gatgtaggct gcgcacagtg
     1321 tccaccagtc atcttcatcc tcaaccaacc ctggaattct gtcgtattca ttgggcttct
     1381 ggcgatactc ggcagccaag acgacacggt cgagcggcat gaagcagaca cttccttccg
     1441 caacgattct gcgctcatcg gtgaatccca gaagaccaat atgcgccgcc tccgttgatc
     1501 gaagtcctgc gttgagcatc agcggtcttc cacagaggct cagcattttc gaagcataga
     1561 gcgtatagtc caagccatca ttgtccttga tgccgatgct t
//
LOCUS       DAOQUC010001080         1600 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_104707_length_1600_cov_1.972816, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001080 DAOQUC010000000
VERSION     DAOQUC010001080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1600
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104707_length_1600_cov_1.972816"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1600)
                     /locus_tag="PKH07_15730"
     CDS             complement(<1..>1600)
                     /locus_tag="PKH07_15730"
                     /inference="COORDINATES: protein motif:HMM:NF020121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase trimerization
                     domain-containing protein"
                     /protein_id="HNT36437.1"
                     /translation="RFFRLGHSAVGPDTFFCKDWNDGAWASTFESIEPGAEYCATVQV
                     KTQDALDYMVALNIAWTADNHAPSVEPLDATVDTGGEWRQLRLNATAPDWAQRGTLNL
                     VLKGSGTALWKQPSVRKVSSPLSGGDRHLKCGETDRELLVNPDFSVKRGQVDPLGWSK
                     TVWHGQFQHSLVRLKREKTRALSLSASAPECGYGNPSAVWYDFDTFSMESYAATMDHW
                     AVEIKKCDPSREVMHYLGFNLGLLGQWDDLSLTQRPDIFLANSPHTDVCGLQLCAGKG
                     DYHYATITLDLARKYGKPMVATDLQDFTHGLYVGFNSLNRTSLACVAHGMSGCYYYCW
                     EDPSPDYSWHPTWTKADTSRMVGNMRKALEFTKGARVKTDVAFVHPVQPYCEADPGGQ
                     KSDMLDCMGWYKAVQQAGLCADVYTPYELTRSNTPALDNYRLVVLPDCPMLPKEAASR
                     LVAYVERGGFLLLAGRTPLTDEAGRQLDFPTPPPQAKKDPLTGTVRWEYGSGTLVRKS
                     STEGKPIWDPCAAIGLPATHPLCFF"
BASE COUNT          354 a          458 c          423 g          365 t
ORIGIN      
        1 ggaagaaaca gagggggtgt gttgccggca acccgatagc ggcgcacggg tcccagatag
       61 gtttcccttc cgtggaactt ttacggacaa gggttcccga accatactcc caacgaacag
      121 tacctgtcag cgggtccttt ttggcctgtg gcggcggagt agggaaatcc agttgtcgtc
      181 cggcctcgtc tgttaatggt gttcgtcccg cgagcaacag gaagcctccc ctctcgacat
      241 aggcgacaag tcttgatgcg gcttccttcg gcagcatggg acagtcgggg aggacgacga
      301 gacggtagtt atccaacgct ggtgtgttgc ttcgcgtcag ttcgtaaggc gtgtacacgt
      361 cggcgcagag accggcctgt tgaacggctt tgtaccaccc catacagtcc agcatatccg
      421 acttctgtcc ccctggatcc gcctcgcaat agggctgtac cggatgaaca aaggcgacat
      481 ccgtcttgac gcgagcaccc ttggtaaact caagcgcctt tctcatgttc ccaaccattc
      541 gagacgtgtc cgcttttgtc cacgtaggat gccaactgta atcaggcgaa gggtcttccc
      601 agcagtagta gtaacaccca ctcatgccat gagcgacaca ggccagcgaa gtacggttca
      661 gagagttgaa gccaacataa aggccgtgcg tgaaatcctg aagatctgtg gcaaccatcg
      721 gcttcccgta tttcctggcg agatcgagcg taatggtggc atagtgatag tctcctttgc
      781 ccgcgcacag ttgaagccca cagacatccg tatgaggtga gttcgcaagg aagatatcag
      841 gacgctgcgt gagactcaga tcatcccatt gaccgagaag cccaaggttg aagcccagat
      901 agtgcatgac ttccctgctc ggatcgcact tctttatttc caccgcccag tgatccatcg
      961 ttgccgcgta tgattccatc gagaatgtgt caaagtcata ccagacagcc gagggattcc
     1021 cgtatccgca ttcgggtgca gatgcagaaa gggagagcgc tcgtgtcttt tcacgcttta
     1081 gtctcaccaa actgtgttgg aactgtccgt gccagacggt cttagaccaa cccaatggat
     1141 ccacctgtcc tcttttgacc gaaaaatccg gattgacaag aagttctctg tccgtttcac
     1201 cgcatttgag atgacggtct cctcctgaga gaggtgaaga tactttccga acggaaggct
     1261 gtttccacaa cgcggttcct gaccctttca gtaccaggtt gagcgttcct ctttgcgccc
     1321 agtcaggcgc agtagcgttc aaacgaagtt gtctccattc tccaccggta tcaaccgttg
     1381 cgtccagagg ctcgacagac ggtgcgtggt tgtccgccgt ccacgcaata ttgagcgcca
     1441 ccatgtaatc caacgcgtct tgtgtcttga cttgcaccgt cgcgcaatac tcggcacctg
     1501 gctcgatgct ctcgaacgtg ctcgcccaag cgccatcgtt ccagtccttg cagaagaatg
     1561 tatccgggcc aaccgcactg tgtcccagac gaaagaaccg
//
LOCUS       DAOQUC010001081         1599 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_104751_length_1599_cov_3.321891, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001081 DAOQUC010000000
VERSION     DAOQUC010001081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1599)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1599)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1599
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104751_length_1599_cov_3.321891"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..984
                     /locus_tag="PKH07_15735"
     CDS             <1..984
                     /locus_tag="PKH07_15735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36438.1"
                     /translation="TPKALSLVQEGLNSADAVVVDAAVRSLADWQNPDPMAKLKHLAA
                     NSDSQVHRVLAFRGYVRMIGMSDEQKRIALYQQAMTLAERPEDRKMILGAMAETRTKE
                     VLAELARYLDDEALRDEAIAAALKVCGSLGLKEGAEVVAVLKKIQEVSDQESTRNEAG
                     RIQRQIEEFEGYVTAWEIAGPYKEKGKEGPKGLYDVVFAPEKKGKVEWRKVTTDAPEK
                     PWLLDFNKICRESNAAVYARFQVSSPKKQKAQLELGSDDGVKVWINGKFVHGKNDARG
                     FNAGEDKVSIELAEGQNTLIFKVTQGGGDWNLGARIRAEDGNRLPDLKFAE"
     gene            996..>1599
                     /locus_tag="PKH07_15740"
     CDS             996..>1599
                     /locus_tag="PKH07_15740"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNT36439.1"
                     /translation="MSSLAVSRRRFLGTTLLGLGAPLVVPASALGLDGATAPSNRIAL
                     GCVGVGGMGMANLNSFLRFDDVQVVAVCDVDKAHRSAARERVQQHYADSSASGFHDGC
                     DSLEDFRELIARDDIDALMIATPDHWHAIPVIMAAQNGKDIYCEKPLSLTIPEGQAIV
                     NAVKRYGRVLQTGTWRRSRVNCRFGCELVRNGRIGKLHTIK"
BASE COUNT          382 a          394 c          475 g          348 t
ORIGIN      
        1 actccgaaag cattgagtct tgttcaggaa gggctcaatt ctgcagacgc tgttgtggtt
       61 gacgctgcgg ttcggtcgct ggccgactgg cagaatccgg atccaatggc caagctcaag
      121 catctggctg ccaattctga cagccaagta catcgggttc tcgcattccg tggttacgtc
      181 cggatgattg gaatgtcgga cgagcagaag aggatcgctc tctatcaaca ggccatgacg
      241 ctcgccgaaa gacccgaaga ccgaaagatg atccttggtg caatggctga gacacgcaca
      301 aaggaagttc ttgctgaact ggcaaggtat cttgacgatg aagccctgcg cgatgaagcc
      361 atcgctgctg cgctgaaagt ctgtgggtct cttgggctca aggaaggcgc tgaagtggtg
      421 gctgtactca agaagattca ggaagtctcc gaccaggaat ccactcgcaa tgaagcgggc
      481 agaatccagc gtcagattga ggaattcgaa ggctacgtca ctgcgtggga aattgccggc
      541 ccctacaagg agaagggtaa ggaaggaccc aagggcttgt acgatgttgt ctttgcgccc
      601 gagaagaagg gcaaagtcga atggcgaaaa gtcactacgg atgctcccga gaagccctgg
      661 ctgcttgatt tcaacaagat ttgtcgagag agcaatgccg ctgtctatgc ccgttttcag
      721 gtctcttcgc caaagaaaca gaaggcacaa ctcgaactcg gttccgatga tggggtgaag
      781 gtttggatca atggaaaatt cgttcatgga aagaacgatg cccgaggatt caacgcaggt
      841 gaagacaaag tctctattga gcttgccgag ggtcagaaca cactcatctt caaggtgacc
      901 cagggcggcg gggactggaa cttgggtgca cgaatccgcg cggaagacgg aaatcgcctt
      961 cctgatctca aattcgcgga gtaatctgag actcgatgag cagccttgcc gtatcgcgac
     1021 gacgttttct tggcacaacc ctgcttggat tgggtgcacc tcttgtggtg cctgcttcag
     1081 cacttgggct ggacggtgca acagcaccgt cgaaccggat tgcccttgga tgcgtcggcg
     1141 tgggcggcat gggaatggca aatctcaact cctttctgcg cttcgacgac gtccaggtcg
     1201 tcgccgtgtg cgatgtggac aaggcacatc gaagcgcggc gcgggagcgt gttcagcagc
     1261 actacgccga ttcttctgca tctgggttcc atgatggctg tgactcgctg gaggatttca
     1321 gagagctcat tgcgcgtgat gatattgatg ccctgatgat tgccacacct gaccactggc
     1381 atgccatccc cgtgatcatg gcggctcaga atggcaagga tatttactgt gagaagcctc
     1441 tatcgctaac cattccagaa ggacaagcca tcgtgaatgc ggtcaagcgc tacggacgtg
     1501 ttcttcagac aggaacatgg cgtcgttcgc gagtgaattg ccgctttggc tgtgaactgg
     1561 tgcgcaatgg aaggatcggg aaacttcaca cgatcaaaa
//
LOCUS       DAOQUC010001082         1599 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_104774_length_1599_cov_2.729922, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001082 DAOQUC010000000
VERSION     DAOQUC010001082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1599)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1599)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1599
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104774_length_1599_cov_2.729922"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1085
                     /locus_tag="PKH07_15745"
     CDS             <1..1085
                     /locus_tag="PKH07_15745"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNT36440.1"
                     /translation="LGSVRPWLGRATAEGLLDPIRETPDQKTLQQWLYQAMVALSQDG
                     ITAVRTPDGVAAFRAYQCLLQQDRLPVRIGFYYPDGYVDSLARTGVRAPFGNDRLAVC
                     GLKAFIDGALGSQTAEMFDCYEKAPDNLGISTMSDSEVLDLFTKATEAGLPVAIHAIG
                     DKANHRVLDVAERVRKSLPNIPWPRHSIEHAQLLTREDIARMAEVGLIASMQPLHVSG
                     DWKMADRHWGQRSKGAYAFRSLLNVRARLAFGSDCPVEEASPMLGMRAAVTRTDLDCQ
                     PEGGWYPEEKLTPMEALAAYTLQGSYAMGFESQIGSLAEGKKADLVVLDCDLLASDPF
                     EWSSERVVLTMMNGQITHHRGDLKLE"
     gene            1092..>1599
                     /locus_tag="PKH07_15750"
     CDS             1092..>1599
                     /locus_tag="PKH07_15750"
                     /inference="COORDINATES: protein motif:HMM:NF016350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00300 family protein"
                     /protein_id="HNT36441.1"
                     /translation="MQSKKIKLVGHIINNLSLSKVLDAILERGGDFRFDELEIGHSEK
                     DISHASLEVIAPDQETLDRILSVISKEGAQETDERSVQIRQTEKDGAVPEDFYSTTNL
                     ETEIFLNRKWIAVEDIEMDCIIVVSPNKVSARCVPIADIRRGDWVVVGHEGIRVHPLR
                     RDRPAVRGG"
BASE COUNT          369 a          419 c          459 g          352 t
ORIGIN      
        1 gtcttggctc agtgcgacca tggcttggta gagccactgc tgaaggtctg cttgatccaa
       61 ttcgcgaaac acccgaccag aagacgctcc agcagtggct ctaccaagcc atggtcgcac
      121 tgagccaaga cggcatcact gccgtcagaa cgcccgatgg cgttgcagcg tttcgcgcct
      181 atcagtgcct gctgcagcaa gaccgccttc ctgttcgcat cggtttctac tatcccgacg
      241 gatatgttga cagcctggcc aggacaggag tccgcgctcc gtttggaaac gatcggctgg
      301 cggtatgcgg tctgaaggcc tttattgatg gagctttggg ctctcaaacc gctgagatgt
      361 tcgactgcta tgagaaggcg ccggacaatc tcggcatcag caccatgagc gattccgaag
      421 tgctggatct tttcacaaag gcaaccgagg cgggtcttcc cgtcgcgata cacgcgatcg
      481 gagacaaagc caaccatcgt gtgctggatg tcgcggagcg cgtacgcaag tcgcttccca
      541 acattccctg gccaagacat tccattgagc acgcacagtt gctcacacgg gaagacattg
      601 ctcggatggc agaagttggc ctgattgcct ccatgcaacc tcttcatgtt tcgggcgact
      661 ggaagatggc cgatcgccac tggggacagc gatcgaaggg tgcctacgcc ttccgatcgc
      721 tgttgaatgt gagagcacgg cttgcttttg gatcagactg tccggtcgaa gaagccagcc
      781 ccatgctggg gatgcgtgcc gctgtcactc gaaccgactt ggactgccaa cctgaaggcg
      841 gttggtatcc agaggagaaa ctgacaccaa tggaagccct tgccgcgtat acactgcaag
      901 gttcttacgc catggggttc gagtctcaga tcggatccct ggcagaagga aagaaggctg
      961 accttgttgt tctggactgc gacctgttgg caagcgatcc gtttgagtgg tcgtcagaac
     1021 gggttgtgct caccatgatg aatggtcaga taacacacca tcggggcgat ctcaaactgg
     1081 agtaacacgt catgcagagc aagaagatca aactggttgg gcacattatc aacaatctga
     1141 gtctctccaa ggttctcgat gcgattcttg aacggggtgg agattttcgc tttgatgaac
     1201 tggagatcgg gcattcggag aaagacatca gccatgccag tctcgaagtg atcgctccgg
     1261 atcaggagac acttgatcgc atcctgagtg tcatttcgaa ggaaggcgca caggaaaccg
     1321 acgaacgatc ggttcagatt cggcagacgg aaaaggatgg tgccgttcct gaagatttct
     1381 actcgacgac aaatcttgag acggaaatct ttttgaaccg aaaatggatc gcagtcgaag
     1441 acatcgaaat ggactgcatc attgttgtca gcccaaacaa agtatcagcg cgctgtgttc
     1501 ccattgccga tatccgccgt ggagactggg tcgttgttgg tcatgaaggc attcgtgtgc
     1561 atccgctgcg ccgagatcga ccggctgtcc gaggcggtc
//
LOCUS       DAOQUC010001083         1599 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_104786_length_1599_cov_2.434585, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001083 DAOQUC010000000
VERSION     DAOQUC010001083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1599)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1599)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1599
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104786_length_1599_cov_2.434585"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            426..>1599
                     /locus_tag="PKH07_15755"
     CDS             426..>1599
                     /locus_tag="PKH07_15755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36442.1"
                     /translation="MNGIHLALAAVLITSMAQADSFRRWESNIEEHQDQLGLWTDDGL
                     LLVLDKADASARTMQIGSVSFPIHAPLIRFEEVRENPAAPNLLDGSLTETWSISEDII
                     EDSANGHSGEWIRVAGSDTERPRANLSLNQKEPQPLVVSGWCQAQVKGEAFGWWNRNL
                     AINARVRYSKDAGAMPEQSAYFGQYDHPPQFSKRIICPDRPIQDVELDLSVLGGDCVA
                     WYRDIQVQAAAYRKLIPTDPPERLDTTIQQLFTIADGSLRGTILFQPTSGFLKIQCLL
                     ESTEPVDRALTGYVTIPFDAVGGVWHDDIRNSREIQSGEIYRNSDWYGAGRDGFFSRY
                     PFACVETAEGSGLAIATSVLEPRVFQAEYDALNKQLRLRFDLGLSPDAGRWANRGSF"
BASE COUNT          360 a          389 c          469 g          381 t
ORIGIN      
        1 acctcattca cctgtggcag ccatttaaga actttacgag cgaagagttc agtgaactga
       61 gagattggtc taaaggaaaa ccgtgcgtgg ctgcggctgt cttcatgaag aaccgcttcc
      121 aggttaagca cctcaatgat aatgatttcg cttagcaatg ggaacgacga cgagatttcc
      181 gagcagcgat tggagccgtc aggggaactg ccgttcagaa aaggcgtcgg tccgcgtgag
      241 aggaaatgtt cgatcgctat cagaagtcta acgggttcat ttgtgcccgt cctgaggctt
      301 tagacagaca gtgtctcctg gtaatcagag gcacgttgcc tgccaatact atcggagtgc
      361 taagatgatc gtgaactcca tcttgggctg acgatttcgg atgacggtgc ttgtaaaggt
      421 ctgacatgaa cggaattcac ttggcacttg cagcggttct gattacttca atggcgcagg
      481 cggattcttt tcgccgttgg gaatcaaaca tcgaggagca tcaagaccag cttggtctct
      541 ggacagatga cggtctgctg ctggtgcttg acaaggcgga cgcatctgcc aggacgatgc
      601 agatcggatc ggtttcattt ccgattcatg cgccgctcat ccgttttgag gaagttcgcg
      661 agaatcccgc cgcgcccaat cttctcgacg gcagtctgac tgagacatgg agcatttccg
      721 aggacattat cgaggattca gccaacggtc actcaggcga gtggattcga gttgcgggtt
      781 cggacaccga gcgaccgcga gcgaacttgt cgctgaatca aaaagaacct caaccgctcg
      841 tggtttccgg ctggtgtcag gcgcaggtca agggagaggc gttcgggtgg tggaacagga
      901 atctggcaat caatgcccgc gttcgatatt ccaaggacgc tggagcgatg ccggagcagt
      961 cagcctactt cgggcagtat gatcatccgc cccaattcag caagcgcatc atttgtcctg
     1021 acagaccaat tcaggatgtg gagttggatc tctccgtact tggtggggac tgtgttgcct
     1081 ggtatcggga catacaggtt caggcagcag cctacagaaa gctgatccca accgatccac
     1141 ctgaacgtct cgatacaacc attcagcagt tgttcacaat cgccgatggc agtcttagag
     1201 gaacgattct gtttcaaccg acaagtggat tcctgaagat tcagtgcctg ttggagagta
     1261 cggagccggt tgaccgcgcc ttgaccggtt atgtgaccat ccctttcgat gcggttggcg
     1321 gtgtatggca tgacgatatt cgcaattccc gggagataca gtcaggcgaa atctaccgca
     1381 actccgattg gtatggcgct ggacgagatg gctttttttc gcggtacccc ttcgcgtgtg
     1441 tggagacggc ggaaggttcg gggctcgcca tcgcgacatc cgttcttgag ccgagagtgt
     1501 ttcaagccga atacgatgcg ttgaacaaac agttgcggct gcgttttgat ctggggctga
     1561 gtccggatgc aggtcgttgg gcgaatcgag gctctttca
//
LOCUS       DAOQUC010001084         1598 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_104902_length_1598_cov_2.457550, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001084 DAOQUC010000000
VERSION     DAOQUC010001084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104902_length_1598_cov_2.457550"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            158..1408
                     /locus_tag="PKH07_15760"
     CDS             158..1408
                     /locus_tag="PKH07_15760"
                     /inference="COORDINATES: protein motif:HMM:NF013151.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36443.1"
                     /translation="MAELTVHRFPFQMPNALADFDPAPFIEQSLKWVDSIVGSNEKVA
                     LSASGGVDSTTLAFLLKDVLGGRLRLFFINDGFRRIMDGREEYEVTADMFKDFPHFEV
                     IHTRDIVLPWFEGVQDGTMKREMFRSRYTITSNKHIASLGADWIADGTIAPDIVMTDQ
                     NRQIQHNVNLPYNMNKLEPLSSLYKPHVRRVAIHLGIPQDFAMRIPCPGPAQLLRVGG
                     AFSAAKLQISKQASDIVEQRVRSWCTHNWGASFHYDEATGVRTPFQYFGTCMDPEMEL
                     DADLSELASKALGSDVTCHRMKTQGMWIDPSVKEQARKLYAPIIWIQGPAIEHDSMIK
                     LTEIIWTQKKLPRVLYEVFDSKNSGFPVGIKIVESEDVKTAWPMDVDFEYLRSMGEEI
                     AAQTKASKVAFDISKRPPATIELF"
BASE COUNT          399 a          434 c          396 g          369 t
ORIGIN      
        1 ctccgctttg cttccgaagc atatcctggc tctatcgcca gtgtaactga gctggtacca
       61 cccccagtgc cgatgagcag gtgcctcttg ctatgcgctg accttccgtc tattatccgt
      121 ttattctgtg cattttcgag aaaggactca gacagacatg gcggaattga cagttcatcg
      181 cttcccattc cagatgccca acgcgcttgc agactttgat cctgccccct tcattgaaca
      241 gtctctcaaa tgggtggaca gcattgtcgg ctcgaacgag aaagttgctc tttctgcatc
      301 aggtggagtg gacagcacaa cacttgcttt tctcttgaag gatgtactcg gcgggagact
      361 ccgtctattc ttcatcaatg acggtttcag aaggatcatg gacggtcgag aggaatatga
      421 agtcactgct gacatgttca aagactttcc gcatttcgag gtgattcaca cacgggatat
      481 tgtgcttccc tggttcgaag gcgtccagga cggcaccatg aaacgagaga tgttccgcag
      541 tcggtacacc atcacttcaa acaaacacat tgcatcactc ggagccgatt ggattgcgga
      601 tggaaccatt gctccggaca tcgtcatgac cgaccagaac cggcagatcc agcacaacgt
      661 caatctccct tacaacatga acaaactcga gcctctctct tcactgtaca aaccgcatgt
      721 caggagagtt gccatccacc tcggaatacc ccaagacttc gccatgcgca ttccctgtcc
      781 cggcccggcg cagttgctcc gtgtgggcgg agcattcagc gctgcaaaac ttcagatttc
      841 aaagcaggcc tcagatattg tggaacagag agttcgatcc tggtgcacgc acaactgggg
      901 ggcttccttc cattacgatg aagccacggg agtccgaacg cctttccagt acttcggcac
      961 atgcatggat ccggagatgg agttggatgc cgacctcagc gagttggctt caaaggcgct
     1021 cggttccgat gtcacctgcc acaggatgaa aacgcagggc atgtggattg acccatcggt
     1081 caaagaacag gcgagaaaac tgtacgctcc aatcatctgg attcagggac ccgcgatcga
     1141 acatgactca atgatcaaac taactgagat catctggaca cagaagaagt tgccccgtgt
     1201 gctctatgaa gtcttcgact caaagaactc aggattccca gtcggcatca agattgtcga
     1261 aagtgaagat gtcaagaccg catggccaat ggacgtggat tttgagtacc ttcgttcgat
     1321 gggcgaggaa atcgctgctc agacaaaggc atcaaaggtt gcctttgata tcagcaaaag
     1381 accccctgca accatcgaac tattttgatt gctgcatccg aatggcagag tacggaaagt
     1441 cttcttaagc ccataagctc tacgtcaggc agaaggcgcc catcacccca gaagtcgggc
     1501 tctgcccacg gctgggtaca ttgttcggcc aaatcacgga ccgatgcgat gaatgtcacc
     1561 caagctggtt gacattcatc gcatcggtcc gtgatttg
//
LOCUS       DAOQUC010001085         1595 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_105181_length_1595_cov_2.873377, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001085 DAOQUC010000000
VERSION     DAOQUC010001085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1595
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105181_length_1595_cov_2.873377"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..717)
                     /locus_tag="PKH07_15765"
     CDS             complement(<1..717)
                     /locus_tag="PKH07_15765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthetase B"
                     /protein_id="HNT36444.1"
                     /translation="MTRIGKSFLLLGLVVAVFHPVAFAQMTLVPMDQTQTDHLKAYGL
                     AYWMLQAPRGVNVEWLLNYRGGSFLIKDASIAPQEATLRGVRTEIISESTANQIHQLI
                     EAENMDVVLLEKAPKIAIYIPPSAEPWDDAVTLALEYAKIPYTEIWDDEVLAGKLKEY
                     DWLHVHHEDFTGQFGKFYANFRNTSWYQEQVRIAQEAATRAGFTKVSQHKNAVTWEIV
                     KYITDGGFLFSMCSACDSFDI"
     gene            925..>1595
                     /locus_tag="PKH07_15770"
     CDS             925..>1595
                     /locus_tag="PKH07_15770"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HNT36445.1"
                     /translation="MPYSKLIFQSRRSPVFSVRGIVAASQPLAVQAGVSILEKGGNAA
                     DAAVATAAALNVTEPGSTGLGGDCFALFYDGVTGRMSALNGSGRAPKALTLELLSSQG
                     FGKELPKYHPHVVTVPGACAGWFDLIERHGTLSMRETLAPAIRLAEEGFPVAPITAHG
                     WQGSIKKLMDSSPGGRELTVEGRAPHVGEVFRNPTLSRTLRLIAEQGRKAYYEGEIAE
                     RIIKTL"
BASE COUNT          366 a          392 c          451 g          386 t
ORIGIN      
        1 gatgtcaaac gaatcacagg cagaacacat ggagaagaga aatccaccat cggtaatgta
       61 tttgacgatc tcccatgtga cagcgttctt gtgctgtgag actttggtga aaccggcgcg
      121 tgtggcagct tcctgggcga tgcgtacctg ctcctgatac caactggtgt ttctgaagtt
      181 tgcgtagaac ttgccgaact gtccggtgaa atcctcatga tgaacgtgca gccaatcgta
      241 ctccttcagc tttcccgcaa gcacttcatc atcccagatt tcagtgtagg gaatcttggc
      301 gtattccagc gcaagcgtga ccgcgtcatc ccaaggttcg gcactgggtg gaatgtagat
      361 ggcgattttg ggtgcctttt cgagaagcac aacatccatg ttttcggctt cgatgagttg
      421 gtgaatctga ttcgcggtgc tttctgagat gatttcggtg cgaacgcccc gaagcgttgc
      481 ttcctgtggg gcgatgcttg catccttgat caggaacgac ccgcctcgat agttcaggag
      541 ccattcgaca ttgacgcctc gcggagcctg cagcatccag taggcgagtc cataggcttt
      601 caggtgatcg gtctgcgtct gatccatcgg caccaaggtc atctgggcga atgcgacggg
      661 atgaaaaact gccacgacaa gacccagcaa cagaaacgac tttccaatac gtgtcattgc
      721 acttcctttt tgggcagacg agaggttctg cctcgtgaat gcagtgtgaa gccgcatcaa
      781 cgcttatatg acgtaagaat agcacaggga ttcattgtgg ccaaaactgg atgacgaaga
      841 ctttgcccca gctatcataa tggactttgt gttttgatgg gtagatgcta gatttcaggt
      901 atctaatttg caatggagac cgagatgcct tacagcaaac tcatctttca gtcgcgacga
      961 tcaccggtgt tttccgtcag gggaattgtg gcggcgagtc agccccttgc ggtgcaggcg
     1021 ggtgtttcta tcctggaaaa aggcggaaat gctgcggatg cggctgttgc cactgccgcg
     1081 gcgctgaatg tcacggaacc aggaagcact ggcttgggtg gtgactgttt tgcgttgttc
     1141 tatgatggcg taacgggacg gatgagcgct ctgaatggct ccggacgcgc tccgaaggct
     1201 ttgacgcttg aactgctctc aagtcaggga ttcggaaagg aactccccaa gtaccatcca
     1261 cacgtggtaa ccgttccagg agcctgtgcc ggttggttcg atctgatcga acgccatgga
     1321 acactttcaa tgcgcgagac tctggctcct gctatccggt tggccgagga aggctttcca
     1381 gtggctccga tcacggctca tgggtggcag ggttcaatta agaaacttat ggattcctct
     1441 cctgggggca gggaattgac agtagagggt cgagcacctc acgtcggtga agtgttcaga
     1501 aatccaacgc tttcccgaac gctcagactc atcgcggaac aaggtcgcaa agcgtactac
     1561 gaaggagaaa tcgccgaaag aatcattaag acgct
//
LOCUS       DAOQUC010001086         1594 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_105314_length_1594_cov_2.705653, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001086 DAOQUC010000000
VERSION     DAOQUC010001086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1594
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105314_length_1594_cov_2.705653"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..724
                     /locus_tag="PKH07_15775"
     CDS             <1..724
                     /locus_tag="PKH07_15775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015891723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HNT36446.1"
                     /translation="PVHVAVIMDGNGRWAKQRHLPRIEGHRAGVKSVQEVVSSAREMG
                     IGVLTLYAFSSENWKRPRTEVSALMLLLRQFMKSELKKMLDNDIRFNVIGRTHLLPDA
                     VQKGIEQTRKATENCRGMTLNLALNYGARSEIEDAFASIVSKIQAGQLQPTQVDADLI
                     SQHLYTGDFPDPDLLIRTSGELRISNFLLWQIAYAELWFTDVLWPDFRREHLAQAVLD
                     FQKRQRRFGDVVSKGGNEPTHE"
     gene            717..>1594
                     /locus_tag="PKH07_15780"
     CDS             717..>1594
                     /locus_tag="PKH07_15780"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HNT36447.1"
                     /translation="MNRLPASQSKGEASTQRKSSSLPKRILSSCAFVPILYGAMFTGW
                     GPEIHLIVASVIQGWAMWEFFTIAERKGVRVPLTILIILGVALQIVGYLGFRGVDVRA
                     PLTLLAVSVPSMLFIWQMSRGVDGALRDVSAALLGFVYASVPLGLLFAIRELPNGAML
                     IMLLLTANFCSDIGAFFVGSHYGKHKLCPTISPKKTVEGAFGAVAGALLGTLAMGFLV
                     QAINGEPGIFLGISRPGPTRYLHATALAVLLSIFGMIGDLGESLLKREAGVKDSGNLF
                     PGHGGILDVIDALLFNT"
BASE COUNT          334 a          395 c          462 g          403 t
ORIGIN      
        1 tcccgttcac gtggcagtga tcatggatgg caatggccgg tgggctaaac agaggcattt
       61 gcctcgtatc gaaggacatc gggctggggt caagtccgtt caggaggttg tcagctcggc
      121 cagagagatg gggatcggag tcctgacgct ctatgcgttc tcttccgaga attggaagag
      181 acctcgaaca gaggtttcag cgctcatgct tcttctgcga cagttcatga aaagtgagct
      241 gaagaagatg ctggataacg atatccgctt caatgtcatt ggtcgaacac atcttcttcc
      301 tgatgcggtt cagaaaggta tcgagcagac caggaaagcg actgagaact gtcggggaat
      361 gacgctgaac cttgcgctca actatggggc tcgatctgag atcgaagatg cgtttgcgag
      421 cattgtttcc aagatacaag cgggtcagct gcaaccgaca caggtggatg cggatctgat
      481 tagtcagcat ctttacaccg gtgacttccc tgatcccgat cttctgatac gtaccagcgg
      541 ggaactgcgt atcagcaact tcctgctctg gcagatcgcg tacgcagagc tctggtttac
      601 cgacgtcctc tggcctgatt tccgacggga gcatctggct caggctgtgc tggactttca
      661 gaaacgacag cgacgttttg gagacgttgt ctccaaggga gggaacgagc ccactcatga
      721 atagactgcc ggcgtctcaa tcaaagggag aagcatcaac tcagagaaaa tccagttctc
      781 taccaaagcg gatcctgagc agttgtgcat tcgttccgat cctctatggt gccatgttca
      841 ccggatgggg gcctgagatc catctcatcg tcgcctctgt gatccaggga tgggcgatgt
      901 gggagttctt cacaatcgcg gagcgcaagg gtgtgcgcgt tccactgaca atcctgatca
      961 tccttggtgt ggcacttcag atcgtcggat atcttggctt tagaggagtg gacgttcgag
     1021 cgccacttac tttgctggct gtttcggtgc cgagcatgct tttcatctgg cagatgtccc
     1081 gaggtgtgga tggggcgttg cgggatgtgt ccgcagcact cctgggcttt gtctatgctt
     1141 cggttccatt ggggctactg tttgccattc gggaattgcc caatggagcc atgcttatca
     1201 tgcttcttct tacggcaaac ttttgttcgg atatcggagc gttctttgtc ggaagccact
     1261 acggcaaaca caaactctgc ccaacgatta gcccgaagaa gacggtggaa ggcgcatttg
     1321 gagccgttgc aggcgctctg cttggcactt tggcgatggg ttttttggtt caggcgatca
     1381 acggcgaacc aggaatcttt ctgggcattt cccgaccagg gccgacccgg taccttcacg
     1441 cgaccgcgtt ggcggttctg ctttctatct tcggcatgat tggagatctg ggtgagtcac
     1501 ttctgaaacg tgaggcagga gtcaaggact ccggaaatct ctttccagga cacggtggga
     1561 ttctggacgt cattgacgct ctgcttttca acac
//
LOCUS       DAOQUC010001087         1592 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_105526_length_1592_cov_2.778139, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001087 DAOQUC010000000
VERSION     DAOQUC010001087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1592
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105526_length_1592_cov_2.778139"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            259..786
                     /gene="def"
                     /locus_tag="PKH07_15785"
     CDS             259..786
                     /gene="def"
                     /locus_tag="PKH07_15785"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012447791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HNT36448.1"
                     /translation="MADREIFCYGNPVLRRKAKEVKRLDRQLQRLIKTMFSTMYQAKG
                     IGLAAPQIGLSQQIIVVDTQEGKNQKLALINPRITACSADDVDTKKEGCLSVPEIEGQ
                     VTRPCCIQLVGLTADGQEVQVEAEGLLARVIQHEIDHLNGILFVDRIQGMDREILEGP
                     LKDLALKHGAGSGRK"
     gene            783..>1592
                     /gene="fmt"
                     /locus_tag="PKH07_15790"
     CDS             783..>1592
                     /gene="fmt"
                     /locus_tag="PKH07_15790"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HNT36449.1"
                     /translation="MNPSSAIIFLGTSDFAVPSLQRLVADGLPVSLVITRPDRPARRG
                     QRLSGSPILCCAESLGLPVWQPENLQDAESVHRIQEMKPSLLVLVAYGMRVPGDVLQA
                     GALGALNLHPSLLPKYRGAAPMQWALIHAEKMTGVTTMYMDEGLDTGDIVYQENAALV
                     PGETYGEVSSRLSVQGAALLSRTVQDVLNGTAPRKVQDNALATKAPLLKPEQERLRWD
                     QEATRVAGWICGLSPRPGAYTLDRERRLKLLRARPLEDVPELVSVALPEQFV"
BASE COUNT          366 a          416 c          454 g          356 t
ORIGIN      
        1 tacgggagga tactacgaaa tcctctggtg ggctctcagt ttcggcgaca aggcagaagt
       61 catcaaaccc gaaaagatgc gaacggaaat aaagaccatt cttggtcgcg ccctggggaa
      121 ttattgaact gtctagtcca gcatgcacag ctgtcgtcgt ggcatctgcg cctcatgaac
      181 gcctttctct tgacaacaca acggttaaga cgtttactac ctttgttgcg ttcatagtca
      241 ggagggtttg tttggagcat ggctgatcgc gagatattct gttacggtaa tccggtcttg
      301 cgtcgcaagg ccaaagaggt caaacgtctt gaccgacagc tgcagagact catcaagacg
      361 atgttttcga ccatgtatca agccaaaggg atcggcttgg ccgcaccgca aatcggtctg
      421 agccaacaga tcatcgttgt ggacacgcaa gagggcaaaa atcagaaact cgccttgatc
      481 aacccaagaa tcaccgcctg ttcagcggat gacgttgaca caaagaaaga aggttgtctc
      541 tccgtgcctg aaatcgaggg acaggtcact cggccttgct gtattcagct ggtcggcttg
      601 acggcggatg gccaggaagt ccaggtggaa gcggagggac ttcttgcccg cgtaattcaa
      661 catgagattg accacctcaa cggaatcctc tttgtagacc gaattcaggg catggacagg
      721 gaaatcctgg aaggtccgct gaaagacctg gcgctgaaac atggagcagg ctcaggtcgg
      781 aaatgaaccc ctcaagtgcc atcatcttcc ttggaacatc agactttgcc gttcccagtc
      841 tgcagcgact ggtggcggat ggtcttcctg tttctctggt cattactcga ccggatcgac
      901 cggcgcgtcg aggccaacgt ctgagtgggt ctcccatcct ctgctgcgca gaatcgttgg
      961 ggcttcccgt ctggcaacct gaaaatcttc aggatgcgga gtctgtccac cgaattcagg
     1021 agatgaagcc atccttgctt gtgcttgtcg cttacgggat gagagtccca ggcgacgtac
     1081 tccaggcggg ggctttggga gcactgaact tgcatccatc actgctgcca aaataccggg
     1141 gtgctgctcc catgcaatgg gcgttgattc atgctgagaa aatgacgggt gtgaccacca
     1201 tgtatatgga cgagggcttg gacacaggag acattgtcta tcaggaaaac gccgcgcttg
     1261 ttcctggcga gacctatggc gaggtgtcct cgcgcttgtc cgttcagggt gcagccttgc
     1321 tcagccgaac cgttcaggac gtgctcaacg gtacggcacc gcggaaagtt caggacaatg
     1381 cgttggcaac aaaggcacct ctcttaaagc cggagcagga acgactccgc tgggatcagg
     1441 aagccactcg tgttgctggt tggatttgtg gactctcacc cagacccgga gcctatacac
     1501 tggatcgcga acgtcggctg aaactgcttc gagcgcgccc gctggaggat gttcccgaac
     1561 tggtaagcgt tgcgcttccg gagcagttcg tt
//
LOCUS       DAOQUC010001088         1592 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_105547_length_1592_cov_2.439818, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001088 DAOQUC010000000
VERSION     DAOQUC010001088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1592
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105547_length_1592_cov_2.439818"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1404)
                     /locus_tag="PKH07_15795"
     CDS             complement(<1..1404)
                     /locus_tag="PKH07_15795"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HNT36450.1"
                     /translation="MTESSKRAFSSGQFCCQRILSFVLLLLGLVYSSPAHAQVLPGPY
                     ESLPFEEAYIIEAWYDLLSGTGIADWTGYTGTSWVSPNAYNGHTGTDYALETGTPIYA
                     PTSGQVAALVTNVPENSHPSGYGNYVRLAIDGTSPMGENLDLVLAHLLPNVPVSVGQR
                     VNRGDVVGYSDNTGNSTSEHLHFESKIRNGAYRCPFYYAHFKYPVMFNPNGIHQMGHI
                     VRIKIENTPIRSEMLETSVEISNAYQGQMFFSSYWQRGYYRVFIPNNRTYRFGWLKAT
                     EVQEVFAGSVLQTLPDDGEYVHTAHLANPYSIRADPTETSETIGQIFFGGARVVADET
                     QSGWYRIPVPDGSRWGWIKPDARMVVYPDLCSPEVDLSVLRRQDFPIRESFSTLGRSM
                     FGRPKFNRGVVKEFSPSSPNGDGKAVFITDATNSGNGVCESVIAGRVEHRNYFVQVDV
                     YLDYKPEQRVSDLAALRV"
BASE COUNT          418 a          431 c          380 g          363 t
ORIGIN      
        1 cacgcgtagc gcggcaaggt cgctgacgcg ttgctcgggt ttgtaatcga gatagacatc
       61 cacttgaaca aagtagtttc gatgctccac cctaccggca atgacagact cacagacccc
      121 gtttccggaa ttggttgcat cggtaataaa gactgccttt ccatccccat ttggggaaga
      181 gggactgaac tctttcacaa cacctcggtt gaacttgggt ctgccaaaca ttgaccgccc
      241 caaagttgag aatgactccc gaattggaaa gtcctgtctc ctaagaacgc tcaaatccac
      301 ctcaggactg caaagatccg gataaacaac catccgcgcg tccggtttga tccaccccca
      361 tcgggagcca tctggaacgg gaatcctgta ccaccccgac tgggtctcgt cggcaacgac
      421 acgagcacca ccaaagaata tctggccaat ggtttcggaa gtctctgttg gatcagctcg
      481 aatgctgtac gggttggcaa gatgcgccgt gtggacatat tctccatcgt ccggcaaggt
      541 ctgcagaacc gatcccgcaa aaacttcctg cacctcagtg gctttcagcc agccaaaacg
      601 atatgtccga ttgtttggaa tgaagactcg atagtaacct cgctgccagt acgaagagaa
      661 aaacatctga ccttgatacg cgttgctgat ctcaacgctc gtttccagca tctccgatcg
      721 gatcggcgta ttctcgatct ttatcctaac aatgtgaccc atttggtgta ttccgtttgg
      781 attgaacatg actggatact tgaaatgggc gtagtagaac ggacagcgat atgctccatt
      841 gcgaatcttg ctttcaaaat gcagatgctc ggaagtcgag ttgcccgtat tgtcagaata
      901 gccgacaacg tctcccctat tcactctttg ccccaccgaa actggcacat tcggaagaag
      961 atgtgccaac accagatcaa gattctcgcc cattggagac gtcccatcaa tggcgagcct
     1021 gacataattt ccatagcccg aggggtgcga attctctggg acattggtca caagcgcagc
     1081 aacctgcccg gacgtcggtg catagattgg agtgcccgtc tccaatgcgt agtccgttcc
     1141 ggtgtggcca ttgtaggcgt ttggagaaac ccacgatgtg ccagtgtaac ctgtccagtc
     1201 ggcaatccct gtgcccgaga gaagatcgta ccatgcctcg atgatatagg cttcctcgaa
     1261 tggaagagac tcatagggac ctggcagcac ttgagcgtga gcaggtgagg aataaactaa
     1321 tcccaagagc aatagaacaa aagacagaat gcgttggcaa cagaactgac cactcgaaaa
     1381 agcgcgcttt gaggattcag tcattttcac caccatagga gtttgttggc atctatgcca
     1441 cgacaagcct atcagacatt ccgtccaagg caaatgcgaa acaacttgac aattgttcac
     1501 tcgatatctg accatctacc cgacaacttg cctgacagga gaacactgca cctatgacag
     1561 agaaagatcg ccagaggctc gtcctcttct tt
//
LOCUS       DAOQUC010001089         1591 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_105622_length_1591_cov_3.125651, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001089 DAOQUC010000000
VERSION     DAOQUC010001089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1591
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105622_length_1591_cov_3.125651"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(107..1114)
                     /locus_tag="PKH07_15800"
     CDS             complement(107..1114)
                     /locus_tag="PKH07_15800"
                     /inference="COORDINATES: protein motif:HMM:NF012618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HNT36451.1"
                     /translation="MSADHSAFQKMMLLACFLLFWGAFAHGQPAGSAESDLALGQLTP
                     LHRLRVQDLTHVKSATSCLVTAGRAGVMIWDGNTGRLVKRISLPTEPYLASLDVSSDA
                     MLLGGGMSDGTALLWDLPSGQAVGSLAGHGGVVSRIAFSSDGQTVLTFGSLDRALKSW
                     GVTERNPIAASRGFSEGSECLAFSSDGKKCLMGEATGMARLCQTDSGRPLLALDGHKE
                     AVVCGSLSADGTRALTGSADATARVWDLSKGDESMTLCGHSGAVVACSFSPDGDLVLT
                     GGKDKIAKLWDAATGRQILELRGHTETLLHVGFTLDSSRAVTAGMEGVVLVWDISKVS
                     R"
     gene            1408..>1591
                     /locus_tag="PKH07_15805"
     CDS             1408..>1591
                     /locus_tag="PKH07_15805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde 3-phosphate dehydrogenase
                     NAD-binding domain-containing protein"
                     /protein_id="HNT36452.1"
                     /translation="MAVKVGINGFGRIGRLVLRAAMKNSAVQVVAVNDITDAKTLAHL
                     FKYDSVHGTYAGSVEAG"
BASE COUNT          410 a          477 c          372 g          332 t
ORIGIN      
        1 tcgacattga cacggagttg gatttgcttg tcgcggaaca actggctgcc cgattccccg
       61 acgcccttcc acgacccgtg acaaggtaga aagacgattt ttactgtcag cgcgacactt
      121 tgctgatatc ccaaaccagc acaacgcctt ccataccggc ggtgactgct ctggacgagt
      181 ccaatgtgaa acccacatgc aacagcgtct cagtatgccc gcgaagctcg agaatctgcc
      241 tgcctgtagc tgcatcccaa agtttggcaa tcttgtcctt gccccccgtc aataccaaat
      301 caccatcggg cgagaatgaa cacgcgacaa ccgcacctga atggccgcac agcgtcatgg
      361 attcatctcc cttcgacaaa tcccatactc ttgccgtcgc atccgcgctg ccagtcagtg
      421 ctcgagtgcc gtcggcggaa aggcttccac agacaacagc ctctttgtga ccatccaagg
      481 caagcagcgg tcttccggag tctgtctgac acaaccttgc catccctgtc gcctcaccca
      541 taaggcattt cttcccatca gatgagaatg ccaggcactc cgaaccttcg gagaaacctc
      601 tggatgctgc gattggattc ctttcagtca caccccacga cttcagagcg cggtcaaggc
      661 ttccaaaagt caggaccgtc tgtccgtctg atgagaatgc gatccgagag acgacacccc
      721 catgtccagc cagagagccc acggcctgcc ccgaaggcaa atcccacaag agagctgtcc
      781 cgtcactcat ccctccccca agcagcattg catccgaaga aacatccaga gaagcaagat
      841 aaggttctgt cggaaggctg attcttttga caagacgacc ggtgttgcca tcccagatca
      901 tcacacccgc ccttccggca gtcaccaggc aagaggtcgc agacttcaca tgcgtcagat
      961 cctgcaccct caaacgatga agaggcgtga gttgtccaag cgccaagtca ctctcggcag
     1021 accccgcagg ctggccatgc gcaaaagcac cccaaaacaa caaaaaacac gcaagaagca
     1081 tcatcttctg aaacgctgag tgatccgctg acattcttcc ttgtcctttt gtctgaatct
     1141 ttctgccgga tattctaaca catgcagact cttgatagaa atgagaccag ttgggatgct
     1201 ctggacaagc acccctacag gagtaggtgc accccgcaca ttcactggga aaaatgcctg
     1261 taccgcacaa ctatcaggcc tctttcaaaa aaactcacga aaaccattga acctcaaaag
     1321 gtaattgtgt tagtcttccc attcatttgg cacattgagt caatgacaaa tggcgaatgc
     1381 caaggaacat ttaggaggat gaaagcaatg gctgtcaaag ttggaatcaa tggatttggc
     1441 cgtatcggcc gtctggtgtt gcgcgctgcg atgaagaaca gcgccgtgca agtggttgcc
     1501 gtcaatgata ttacggatgc caagaccctg gcgcacctgt tcaagtacga ctcggtgcat
     1561 ggaacttacg ccggttcggt tgaagcaggc g
//
LOCUS       DAOQUC010001090         1590 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_105753_length_1590_cov_2.817590, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001090 DAOQUC010000000
VERSION     DAOQUC010001090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105753_length_1590_cov_2.817590"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..995)
                     /locus_tag="PKH07_15810"
     CDS             complement(<1..995)
                     /locus_tag="PKH07_15810"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNT36453.1"
                     /translation="MFDLLIRKGTIYDGSGRKEAIRTTDIGITDGKIKVIGNLADAEA
                     QEIIDASEKVVTPGFIDPHGHSDMSLLANPQAESLVRQGITAQISGQCGASPFPVTEK
                     AKEEHAKDLKELDLDLTWSSMGGYRKAIEYRGIAINSGILVGHGTVRACVCGYDAIKP
                     NESQMKEMVALVDREMSEGALGFSSGLQYSPGHYAEPWELIPLARAAAQKGGIYTTHM
                     RSEGNRLFESIEESIMVAREAGITLEIAHLKSSGKPNWGKMDHALEMIEAAEKEGIDI
                     AFDRYPYLAGSTSMSIYFSPAMLEGGREKMLERISDPKLRPEIRKHMDETVNRALG"
     gene            complement(1241..>1590)
                     /locus_tag="PKH07_15815"
     CDS             complement(1241..>1590)
                     /locus_tag="PKH07_15815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36454.1"
                     /translation="ETDFAVHVWEGSATPSQATYSQSADLLTNVSSPSGGVDVMDDGT
                     VLVGYDPEGWILGFDADHNYLGKLSGGTPTMTQPRGTAFTPNGSVLWIACKSGRVQRW
                     ERVSEIPNGWFVY"
BASE COUNT          329 a          471 c          391 g          399 t
ORIGIN      
        1 cccagtgccc ggttgaccgt ttcatccatg tgcttgcgga tttcaggtct cagtttgggg
       61 tcgctgattc gttccaacat cttctctcga ccgccttcca acattgcggg cgagaagtag
      121 atggacatgg aggtggaacc agcaaggtaa gggtagcgat caaacgcgat gtcaatgccc
      181 tctttctcgg cggcttcgat catctccagg gcgtgatcca tcttccccca gttgggtttt
      241 cccgaactct tcaggtgggc gatctccaga gtaatcccgg cttcacgagc aaccatgatg
      301 ctttcttcga tagactcgaa caggcgattg ccttcactgc gcatgtgggt agtgtagatg
      361 cctcccttct gcgcggcggc tcgggcgaga ggtattagct cccacggctc ggcatagtgt
      421 ccgggcgaat actgcagccc tgatgagaac cccaatgctc cttcgctcat ctctcgatcg
      481 acgagagcca ccatctcttt catctgactt tcgttgggct tgatcgcgtc gtaaccgcag
      541 acgcaggctc gcaccgtgcc atgacccacg agaatgcccg agttgatcgc gatgccccta
      601 tactcaattg cctttcgata gccccccata cttgaccagg tcaaatccag gtccaactct
      661 ttcagatcct tggcgtgttc ctctttcgcc ttctccgtga ctggaaacgg ggaggcaccg
      721 cactggccac tgatctgagc ggtgattccc tggcggacaa gactctctgc ttgcgggttg
      781 gcaagcagcg acatgtccga atgaccatga gggtcaatga atccaggtgt aaccaccttc
      841 tcactggcat caatgatctc ttgggcttcc gcgtcagcga gattgccaat gaccttgatc
      901 ttcccgtccg taattccaat gtctgttgtg cggattgctt ccttgcgacc gcttccgtcg
      961 taaatggttc cttttctgat cagcaggtca aacattttac tccttctctt ctcccagact
     1021 ttgtggctgg atcaactgtt tcttactgtc gggggattat gccacagatt gcccatggca
     1081 cgtcaagcag agcaatctct gcaccaaagc gaatgcactg gttggcagac tgccaaacct
     1141 cgcacagaag acaagtctca aaacacctga tgctgttcgg atgatctgtc cttcaccaag
     1201 aagtcatcgc aattccgtat cgcatcacct atgctcagca ctaatataca aaccagccat
     1261 ttggtatttc gctgactctc tcccaacgct gcactctgcc gctcttgcac gcgatccaga
     1321 gcactgagcc gttgggagta aaggcggtgc cacgaggctg agtcatggtc ggcgttccac
     1381 cggaaagttt tcccaggtag ttgtggtcgg catcaaatcc gagaatccaa ccttcgggat
     1441 cataacctac cagcacggtg ccgtcgtcca tgacatccac cccgccactt ggagaactga
     1501 cgttcgtcag caagtctgct gattgagagt aggtcgcttg agacggtgtc gcactgcctt
     1561 cccagacatg caccgcaaaa tctgtctctg
//
LOCUS       DAOQUC010001091         1589 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_105915_length_1589_cov_1.700782, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001091 DAOQUC010000000
VERSION     DAOQUC010001091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1589
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105915_length_1589_cov_1.700782"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..610)
                     /locus_tag="PKH07_15820"
     CDS             complement(<1..610)
                     /locus_tag="PKH07_15820"
                     /inference="COORDINATES: protein motif:HMM:NF016158.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cryptochrome/photolyase family protein"
                     /protein_id="HNT36455.1"
                     /translation="MVRRKKSVDSSKESVWILPDQLSLDISSLQGRSPNIPIVMVEFQ
                     DREGSVRMHKMRLVFLLSAMRHFRDRLISQGYRVDYYAADDPDEKRAALCREGYLACL
                     KDYVKRNGIERLFVTEPNLFSLDEQIRNSLADCLKIAVELVPTNQFLVSRAQFRNWEQ
                     MQGELVMENFYRMMRQNLDILMDGSDPQGGRWNYDIENRVPPM"
     gene            900..>1589
                     /locus_tag="PKH07_15825"
     CDS             900..>1589
                     /locus_tag="PKH07_15825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36456.1"
                     /translation="MKPKNAEQTSACPNRKTLLSWSLIRFLFVLAFWLTMISILWIRE
                     IQFEFFRNRAGTGNMASPVFSELLRYQYERETVGITALLILPVVEETKLGGEKSIPKG
                     ASWEIRQCGTLEIPLLGTRTPVALAGRMLLDSEFHLLRFDYSLEMPNGLFKTLGAISD
                     QGTLDVQYGFSGNVQTMSFPVPKNGMSMYLAATPLLVHRIPFKPGTKHRIEQFNPFTA
                     AMEEVRISFGQE"
BASE COUNT          378 a          427 c          372 g          412 t
ORIGIN      
        1 ccatgggggg cacacggttc tcgatatcgt agttccagcg gcctccctgg ggatcgctgc
       61 catccataag aatgtccaga ttctggcgca tcatgcggta gaagttctcc atgacgagtt
      121 cgccctgcat ctgctcccag tttctgaact gtgcccgact caccaggaac tggttggtcg
      181 gtaccaattc aacagctatc ttcaggcagt cggcgaggct gttgcggatt tgttcatcca
      241 gactgaacaa gtttggctcc gtcacgaaaa gcctctcgat gccattgcgt ttgacgtagt
      301 ccttcaagca cgccagataa ccttcccgac acagtgccgc acgtttctca tcggggtcgt
      361 cagcggcgta gtagtcaacc ctgtagcctt gactgatgag tctgtcccgg aagtgtctca
      421 tggcagacag aaggaaaacc aggcgcattt tgtgcatccg cactgaacct tctctatcct
      481 gaaactcaac catgacaatc gggatatttg gggatctgcc ctgcagagag gaaatatcca
      541 aactgagctg atccggaagg atccagacgc tttctttcga tgaatcaaca ctttttttac
      601 gacgaaccat gcttcaaaag gttccttgct tcctttgtcg tttgcctatc gtgaggcact
      661 cccacatccc aaatgcgttt gcttctatta cctatactga cgaatcaggg ttctgtcaag
      721 ccctctcatc cacttttcat tgtcattgct ttgtgtcacg gtgtattctt ctctcgaaaa
      781 caggatccaa agatggatcc acttttcacc agaaacgccc tcgaaggagc ggacatagcc
      841 catgtcatgt caggcaaccg ccggctcaaa cgatgcctca gacggacact tttgagacaa
      901 tgaagccaaa gaacgcagaa cagacatcag cctgtccaaa ccgtaagacg cttctgtcat
      961 ggtctctcat tcgatttctt ttcgtccttg ctttctggct gacaatgatc tcaattctgt
     1021 ggatccgaga aatccaattc gaattcttca gaaatcgcgc gggaaccggc aatatggcca
     1081 gtcctgtttt ctcggaactc cttagatatc aatacgaacg agaaacggtt ggaataactg
     1141 cccttctgat tcttccggtt gttgaagaga ctaagctagg tggtgagaaa tctatcccca
     1201 aaggtgcaag ttgggagatt cggcagtgcg ggacactgga aatcccactt ctgggaacca
     1261 gaacgcctgt cgctctggca ggtcgcatgc ttctcgattc ggagttccac ttgttacggt
     1321 ttgattattc tctggaaatg ccgaacgggc ttttcaagac ccttggagcg atcagtgacc
     1381 aagggacgct tgatgtccaa tacggtttct caggcaatgt ccagaccatg tcctttccgg
     1441 ttcccaagaa cgggatgagt atgtacttgg cggctacgcc tctcttggta caccgtatcc
     1501 ccttcaaacc agggacaaaa catcggatcg agcaattcaa tccatttacc gccgccatgg
     1561 aggaagtccg gatctctttc ggtcaagaa
//
LOCUS       DAOQUC010001092         1588 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_106023_length_1588_cov_2.150033, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001092 DAOQUC010000000
VERSION     DAOQUC010001092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1588
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106023_length_1588_cov_2.150033"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            182..>1588
                     /locus_tag="PKH07_15830"
     CDS             182..>1588
                     /locus_tag="PKH07_15830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36457.1"
                     /translation="MTFRSVLLGLFGAAFVCGFTYFNDAVIRQTFFVGNHMPFSVYGG
                     LVVFLLVGQPLLARVSKRLVLSSKEIAVILTLTLASCCIPSSALLRIFTPTLILPHRF
                     AKTDPAWERTKIVSLAPAEMLADVSRNENVVLDGFVLGLGEGDKHISPSRVPWYAWMR
                     TFAFWIPLIVTFWVALIALAVVVHRQWSRHDLLPYPIATFARSLLEPDPHDGISVFRK
                     RAFWIGLFVILAVHANNYLYAWFPSRTFQIKTWLDLHPLASLFPNFNRGGGAALLLWY
                     NRIFPTAVAVAFFLPADVSFSAGISPILFFTVSGVFARYGIPYGGGNYFQPNISRFVN
                     FGAFAGVFLVILYTGRHYYRCVLGRAVGLSGNTEAEPSSVWGMRVFLLAIAGFVLQLS
                     LVGLDWHVGLLFSLGAIVFFLVMGRVIAETGIFFFQPQWFPCVILTGLFGAQAIGLQN
                     LIIVSLVAVLIVTDGRETL"
BASE COUNT          279 a          434 c          408 g          467 t
ORIGIN      
        1 agactaccac tggagcgcct cagtctttct tagcctcgcc ttgcagtctt ctgccaaaca
       61 cagcaaacaa ggctagaaaa tccgcccgac caggctgatc gcttttgcgt tgacaaagaa
      121 tccgtcacca agcaggatta ctcgctgaca ctttatcctg gcgatgtcag gggcatccag
      181 cgtgactttt cgttcagtcc tccttggtct atttggcgct gcgttcgtct gcggcttcac
      241 ttatttcaat gatgcggtca tacgccaaac cttcttcgtg ggcaaccaca tgcctttttc
      301 cgtctacgga gggctggtcg ttttcctgtt ggtcggtcag cctttgctgg ccagagtctc
      361 caaacgactg gttctctcca gcaaagagat tgcagtcatt ctgaccctga cgctcgcatc
      421 gtgctgtatt ccaagttccg ccttgcttcg aatcttcaca cctactttga ttcttccaca
      481 tcggtttgcg aaaaccgatc cagcctggga acgaacgaaa atcgtttctc tcgcaccggc
      541 tgagatgctg gctgatgtca gccgaaacga aaacgtggtt ctggacggct ttgtgctggg
      601 tctgggcgag ggagacaagc acatatcccc ttcgagggtt ccctggtacg cctggatgag
      661 aacgttcgcc ttttggatcc cgttgatagt aaccttctgg gtcgcgctca tcgccttggc
      721 cgtggttgtg catcgccagt ggtcacggca cgatctgctt ccctatccca tcgcgacctt
      781 tgcccgatca ctcttggaac cagatcccca tgacggcatc tcagtttttc gcaagcgcgc
      841 cttctggatt ggtttgtttg tgatattggc agttcacgcg aacaactacc tgtacgcctg
      901 gtttcccagt cgcacgtttc agatcaagac gtggttggat ttgcatcccc tggcctctct
      961 ctttcccaac ttcaaccgag gcggaggcgc tgctctgctg ttgtggtaca accggatatt
     1021 tccgacagct gtggcagtgg cattctttct gcctgcggac gtctcgttct cggctggcat
     1081 ctctccgatt ctgttcttca cagtatccgg cgtcttcgcc aggtatggaa ttccctatgg
     1141 cggtggaaat tactttcaac cgaacatttc ccgatttgtg aattttggtg ccttcgcagg
     1201 ggtgtttctg gtcatcctct acacgggcag acactactac cgttgtgttt tgggtcgtgc
     1261 tgtcgggctt tcgggaaaca ctgaagcaga gccttcatcc gtttggggga tgcgagtctt
     1321 cttgttggcg atcgccggat ttgtcctgca actttctttg gttggtttgg actggcatgt
     1381 gggtttgcta ttcagcctgg gggctattgt gttctttctg gtcatgggta gggtcattgc
     1441 cgagacagga atcttctttt ttcagccaca atggtttccg tgcgtcattc tgacgggact
     1501 ctttggagca caggcaattg ggcttcaaaa cctgatcatt gtttctctcg ttgcggtgct
     1561 tatcgtcacc gacggtcgcg agacactg
//
LOCUS       DAOQUC010001093         1586 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_106235_length_1586_cov_2.548661, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001093 DAOQUC010000000
VERSION     DAOQUC010001093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106235_length_1586_cov_2.548661"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(75..524)
                     /locus_tag="PKH07_15835"
     CDS             complement(75..524)
                     /locus_tag="PKH07_15835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019365688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HNT36458.1"
                     /translation="MELASAPDLPDQPLQQHPEAPQIAGMQESPPEVSAPSPTEGSAY
                     SEEGKASWYHRKFTWRRTASSERYSPKEYTAAHRSLPFGTVVEVTNLNNKKTCTVKIN
                     DRGPRVKGRIIDLSEIAASDLGIIKQGVAPVRIRVIQEPESPVDKAK"
     gene            862..>1332
                     /locus_tag="PKH07_15840"
     CDS             862..>1332
                     /locus_tag="PKH07_15840"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HNT36459.1"
                     /translation="MIDRQILVKLLHVQDLPTLPEVMAEILETIADEASSASDLTVLL
                     EQDHAISARVLRLANSAFYGLRTRVDSIRRAVVVIGFDAVRHLALATTVFDALARRQQ
                     FALVPEDFWMHSLGAAKAAQIIAGRYCREASSDGCFTAGLLHDLGKYVLALVLKG"
     assembly_gap    1333..1342
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1370..>1586
                     /locus_tag="PKH07_15845"
     CDS             1370..>1586
                     /locus_tag="PKH07_15845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36460.1"
                     /translation="MQQELLQLKTTNAEVGQWLAEKWRFPGLISDPIGYRYRLSKYTG
                     DYRANVSVTAAADEISRIAEFGIAGDFN"
BASE COUNT          348 a          371 c          444 g          413 t
ORIGIN      
        1 aggagagtct atgagttgca gttgttgtgt agaagaatgg tttgaccgta tgcctctgaa
       61 gctgtacgta ttggctactt agctttatcc actgggctct cgggttcctg aatcactcgg
      121 atgcgaacgg gtgccactcc ctgtttgatg attcccagat cagaggccgc aatctcgctc
      181 aaatcaatga ttcgaccctt cactcgggga cctcggtcgt tgatttttac ggtgcatgtc
      241 ttcttgttat tgaggttggt cacttccacg acagttccaa aaggaaggct tcgatgtgcc
      301 gcagtgtatt ccttggggga atagcgttct gaggatgcag tcctgcgcca agtgaacttc
      361 ctatggtacc aggatgcttt gccttcctca gaataggctg aaccttcggt tggagacggt
      421 gctgagactt cgggcggaga ctcctgcatc ccggcaatct gtggggcttc aggatgctgc
      481 tggagcggtt gatccggaag atcgggggcg gaggcaagtt ccactcctga cttccttgag
      541 cccatcctta tgacaacccc tgttctgtag ggttttgtcg gcaatatggc acaggatgag
      601 gagataatcc ctacgataag tggtacgaag aaaagcgctt gatgtctcac tctcataggc
      661 tgtctttcct cttcatccga caagagagga agtctcattt gatctacctg ccatgtcaag
      721 agttatccgc tcccaacaaa gaaatgcggt ttttgacttg cattgaacca gaacaggacg
      781 tatccttcaa gaagaaggcg cgttatgtgt gtcttctgaa attggcaaaa gacgtagaga
      841 tgttgctagg acggcggggt aatgatagac agacagatac ttgtcaaatt gcttcatgtt
      901 caggatctgc cgactcttcc agaggtcatg gcagagatac tcgagactat tgccgacgag
      961 gcatcgtcgg caagtgactt gactgtgctc ctggagcaag accatgcgat ctcagcgcgc
     1021 gtgcttcgac tggcaaactc ggctttctac ggccttagaa ctcgtgtgga ttccatccga
     1081 cgtgcggtgg ttgtgattgg ctttgatgcg gtgaggcatc ttgccttggc gacaaccgtg
     1141 ttcgacgcac ttgcgagaag gcagcagttc gccttggtgc ctgaggactt ctggatgcac
     1201 tcgctcggtg cagcaaaggc tgcgcagatt atcgctggca gatactgccg ggaagcctca
     1261 agtgacggat gtttcacggc aggccttctt cacgaccttg gtaagtatgt cttggcgttg
     1321 gtactcaagg gtnnnnnnnn nntcagcacg ttccgacgga tgtgccctga tgcagcaaga
     1381 acttcttcaa ctaaagacaa cgaatgctga agtgggtcag tggcttgctg agaagtggcg
     1441 atttccggga cttatctccg atcctatcgg gtacagatat agactttcca agtatacggg
     1501 agactatcga gcaaacgtct cggtgaccgc agcggcagac gagatctctc gaatcgctga
     1561 gtttgggatt gctggggatt tcaatc
//
LOCUS       DAOQUC010001094         1584 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_106398_length_1584_cov_3.323741, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001094 DAOQUC010000000
VERSION     DAOQUC010001094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106398_length_1584_cov_3.323741"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1543
                     /locus_tag="PKH07_15850"
     CDS             <1..1543
                     /locus_tag="PKH07_15850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36461.1"
                     /translation="LERLNIEWVKIPIRWHNLDYSVLDSLIDHLYSRGLSVVGQLVGT
                     AREAASTQEDWVVGEIGPRFASGPPTDYALWEQFAADITKRYKERIRHWEIWTDPQGG
                     RGPNWRGDAEQYSLLVEHAVRGIKRMDPLAQIINPGLFDQPDDFAKIVTARTAGLTDV
                     VGFETDTLTRNLPAKLMEKWSKVAMEVPIWVTDSRGGNAAQVVQEYVQSRALGLKRLF
                     RYSYRDPEPTRSAGSFVSSDFVPTRAAVAYSGISHLLEETQFIGELDFPGDTVCYIFE
                     KDNMRYYVLWHEDPSLFGGWQWPVGIHLGQWLPDAYDALGDRTKIRLNWKGNTTLWIG
                     EEPFVVTAPKNTRVYPVAARATTVRIPSTEGFLVVEAEQAVARDSEWKVVAGTGLSSG
                     RAVSNQAVAEQYEDAAALEIPFNLERTGNYEVCFAGTPLSRVPWECSAFEWQIDGEKP
                     SPVMVPGIERRVYAEGKAALTPLGSLSLGSGQHVFRISLIQSPTSGSPTHDILIDCLI
                     LRPLP"
BASE COUNT          341 a          440 c          461 g          342 t
ORIGIN      
        1 gttggaacgg ctcaacattg agtgggtgaa gatccccatt cgatggcaca acctggatta
       61 ctccgttctg gactcactca ttgaccacct ctactccaga ggcttgtccg tggttggtca
      121 actggtcggc acagcgcgtg aagcagcctc cactcaagag gactgggtgg tgggtgagat
      181 tggtccacgc tttgcctctg ggccaccgac ggactatgcg ctgtgggagc aatttgccgc
      241 ggatatcacc aaacgctaca aggagcgaat tcgacattgg gagatttgga ctgatcctca
      301 aggaggacgg ggaccgaact ggcggggaga cgccgagcag tactcgctcc tggtggaaca
      361 cgccgtgcgc ggcatcaagc gcatggatcc gctggcacag attatcaacc cgggcctctt
      421 tgaccagccg gacgatttcg caaaaatcgt cacagccaga accgccggac tgacagatgt
      481 cgtcggtttt gagacagaca ctttgactcg gaaccttcct gcaaagctca tggagaagtg
      541 gagcaaggtc gccatggaag tccccatctg ggtcaccgac tcacggggag gcaatgcggc
      601 acaggttgtc caggagtacg tccagtcgag agcgttggga ctaaagaggc tctttcgcta
      661 ctcctacagg gacccagagc caacccgctc cgcaggcagt ttcgtctcca gcgattttgt
      721 tcccacccgt gctgctgttg cttactccgg catctctcat ctcctggaag agacccagtt
      781 catcggtgaa ctggattttc ctggagacac ggtgtgttac atcttcgaga aggacaacat
      841 gcggtactat gtactgtggc atgaagatcc gagtcttttc ggcggttggc aatggcctgt
      901 tggcattcat ctgggtcagt ggctgcccga cgcgtatgac gcgcttggag atcgaaccaa
      961 gattcggctg aactggaagg gaaacaccac gctctggatc ggtgaagaac cctttgttgt
     1021 caccgcaccc aagaacactc gagtctatcc tgttgccgcc agagcaacga ccgtcagaat
     1081 tccgtcaacc gaaggctttc ttgttgtcga agcagaacaa gccgtggcca gggattccga
     1141 gtggaaagtc gttgccggca caggtctgtc atcgggcaga gcggtgtcca accaggcagt
     1201 cgccgagcag tacgaagacg ctgcagcatt ggagattccc tttaacctgg agcgaacagg
     1261 aaactatgag gtctgctttg caggcacacc cttgtcccgc gtgccgtggg aatgctccgc
     1321 tttcgaatgg cagatcgatg gagagaaacc cagtccggtc atggttccgg ggatcgaacg
     1381 tcgggtctac gctgaaggca aagcggcact cacaccgctg ggttccctgt cgttggggtc
     1441 agggcaacac gtgtttcgga tctctttgat ccagtcacca accagcggtt cgcccacgca
     1501 tgatattctc atagactgcc tgattctgag gccgcttcct tgatcgtctg gaccgccagc
     1561 ggactccttt cggcgatctc ggcg
//
LOCUS       DAOQUC010001095         1583 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_106528_length_1583_cov_3.077880, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001095 DAOQUC010000000
VERSION     DAOQUC010001095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1583
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106528_length_1583_cov_3.077880"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1042)
                     /locus_tag="PKH07_15855"
     CDS             complement(<1..1042)
                     /locus_tag="PKH07_15855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36462.1"
                     /translation="MFGILCRFLTVFVSVVVLLSATETAQAKRVTLTFHVSTTGNDAW
                     SGTRATPNRKRTDGPFSSLERAFSEVCTAAAANSAYRMEAVIELQAGCYEIKDTLRIT
                     NEELGSEGNSLTIRGGKGAEVRLVGGKRVEGFEPVSDPEVLAQLDESARGNVVQRNLR
                     QLGIADLGEAGGGGLELFFNDQPMTVSRWPNEEFTKVTGLAGQSPIESHGIKGDSVGV
                     INYEGDRQSRWVAEKDPWAHGYWFWDWSEQRQRIESIDAEKHILTLAKPYHQYGYRLN
                     QWYYGFNLFCEIDSPGEWYLDRTTGMLYFWPPSPVEHGRAVVSVISTVFSFKDISHVT
                     IENLTIEACRGHA"
     gene            1184..>1583
                     /locus_tag="PKH07_15860"
     CDS             1184..>1583
                     /locus_tag="PKH07_15860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36463.1"
                     /translation="MKSRTLGSLSVMTLLLGGVEMAFAQDGWRSALRPAGESGTKVTL
                     ANNGRSSYRIVLPSKPTTQEEKAAEELQRWLGEMTGATLKIVRESGRLRLGPHYISIG
                     RTRMLEQANRQDDNGRHGGDHDGMGKQALIP"
BASE COUNT          366 a          431 c          418 g          368 t
ORIGIN      
        1 cggcgtgacc tcggcaggct tcaatagtca agttctcaat ggttacgtga gagatatctt
       61 tgaacgagaa taccgtcgag atgaccgaca caacggcgcg accgtgctca accggagaag
      121 gaggccagaa atagagcatc cctgttgtgc gatccagata ccattctcca ggtgagtcga
      181 tctcgcaaaa caggttgaac ccatagtacc actggttcaa gcgatagcca tattggtggt
      241 agggtttggc gagcgtgagt atatgctttt cagcgtctat ggattcaatt ctctggcgct
      301 gttctgacca gtcccagaac caataaccat gagcccaggg gtccttctct gcgacccagc
      361 gtgactgtcg atccccctcg tagttgatca ctccgacgct gtcgcctttg atgccatggc
      421 tttcaatggg cgattgtccc gccagtccgg tgacctttgt gaactcttcg tttggccacc
      481 ttgacacggt catcggttgg tcattgaaga acaactccag gccacccccg cctgcttcac
      541 ccaaatccgc aattccaagt tgcctgagat tcctctgaac gacattccct cttgccgatt
      601 catcgagttg cgcgaggact tcgggatctg aaacaggctc aaagccttcg acgcgcttgc
      661 cacccaccag gcggacttcg gctcccttgc caccacggat agttagagag ttaccctctg
      721 aacccagttc ttcgttggtg attcgcaggg tgtccttgat ttcatagcat cctgcttgaa
      781 gctcaatgac cgcttccatt ctgtatgcgc tgtttgctgc tgccgcggtg catacttctg
      841 aaaatgccct ttccagacta ctgaaagggc catccgttct tttgcgattt ggggtggcac
      901 gagttccgct ccaagcatca ttgcctgtag tcgagacatg gaaggtaagc gtcactcgct
      961 tcgcctgcgc cgtttccgtg gcagaaagaa gaacaacaac agatacaaaa actgtcagaa
     1021 acctgcacaa tatgccaaac atctgaacct ccatcaggaa ccaatctgat acaaccgcct
     1081 ctactgtatt ccattggcgg aagttgttca aaccgctgct tgacatcgag tcggcgatga
     1141 ctagactcat acgacagata tccactggag gagaacaatg agaatgaagt cacgcacgtt
     1201 gggctcgctg tctgtgatga ctcttttgct tggaggtgtc gaaatggctt ttgcacaaga
     1261 cggatggcgc agtgcgctga ggcctgcggg agaatcaggc acgaaggtga ctctggcgaa
     1321 caatggccgg agttcctatc gcatcgtcct tccgtccaag ccgaccacgc aagaggagaa
     1381 agccgcggaa gaactgcagc ggtggcttgg tgagatgacc ggtgcaactc tcaagattgt
     1441 ccgtgagagt ggtcgcttga gacttggccc ccactatatc agcatagggc gaaccaggat
     1501 gctggagcag gcgaaccggc aggatgataa tggccgtcat ggcggcgatc acgacggtat
     1561 gggcaagcag gcgttgatcc cct
//
LOCUS       DAOQUC010001096         1581 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_106753_length_1581_cov_3.669069, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001096 DAOQUC010000000
VERSION     DAOQUC010001096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1581
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106753_length_1581_cov_3.669069"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(95..757)
                     /locus_tag="PKH07_15865"
     CDS             complement(95..757)
                     /locus_tag="PKH07_15865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36464.1"
                     /translation="MQPFAPGPTALGENFSQDLLITHAAWLAKSTAPYVPLNGGMPPG
                     LMVAQFDTLTSLLFQYRTQGFSFYEGSPSIGPGRFDLYVSVSQTNPERNRGTPLNPAP
                     VPTRIEDIFVAGNPLARYMSVAGDLAVRKVPFSVMRSTAQERWASSEERNLAKYFAEK
                     IVEQAKEVAANKSAPPEVRKEARNILKDFSLARDPRRLTPMEEYVKSNKGRIIRKKTK
                     LH"
     gene            complement(1180..>1581)
                     /locus_tag="PKH07_15870"
     CDS             complement(1180..>1581)
                     /locus_tag="PKH07_15870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36465.1"
                     /translation="SDFRYTEEVAALTIPYLSLFLQIQGCPQYSLSDDLEPLELVARC
                     TAVQGNYDKFRPFILFDTDTLEGRIRLDRNFEELTESQPMSVFLTAHGLLLQCSNYNQ
                     LSVEKACALLESEFNIQVQRHLLPQDLEESL"
BASE COUNT          377 a          406 c          423 g          375 t
ORIGIN      
        1 gtcttggttc ccttctgaaa cggagtgaaa aattcgagat gagcttcggt ggggagagtc
       61 ggtcggtgca ggtgcatttg gggttcctca actatcaatg taactttgtt ttcttgcgaa
      121 tgatacgtcc cttattggat ttgacgtatt cctccatggg agtcagtcga cgaggatctc
      181 tggcaagaga aaaatccttc aagatgtttc tggcttcctt gcgcacctcg ggaggtgcac
      241 tcttgttagc cgcgacttct tttgcctgtt cgacaatctt ctccgcgaaa tatttcgcaa
      301 ggttgcgttc ttcagatgac gcccagcgct cctgcgcagt agaacgcatc acggaaaatg
      361 gcaccttgcg aaccgcaaga tcaccagcga cgctcatgta cctggccaag gggtttccag
      421 cgacgaagat gtcctcgatc ctcgtcggca cgggcgctgg attgagaggc gtgcctcgat
      481 ttcgttcagg gttcgtctga gaaacggaga cgtagaggtc aaatcgtcca ggtccgatgc
      541 tcggggaacc ctcgtaaaac gagaaccctt gggttctgta ctgaaacaac aacgaggtca
      601 gcgtgtcgaa ctgggcgacc atcagaccag gaggcatacc tccgttcaga ggtacatagg
      661 gtgctgtgga tttcgccaac cacgcagcgt gagtgatgag caagtcttgg ctgaaatttt
      721 ctccaagtgc ggtgggccct ggtgcaaatg gttgcatcgg gcgtcctgga gtccacgtga
      781 cgaaccaagt cacgttccca tctggaaaag accaaaatgg gctcaattgc tcttgttcgg
      841 tatacacagc ctccgaatcc aaatcggctt gtcgacgagt tccaatcacg atcgactggc
      901 gaagtccaac gagctgaccc aagtaaaaat cgggcacgta gatcgtcgaa aggtgtccga
      961 aataacgagt cgcagggctc ctggtgggtg cctctgggac aggcaagtat gcaaccccat
     1021 tgcccccgag cggatcggta ccaatccctt cccaccggac agtatccgtg gctttcgtaa
     1081 gactgttatc aagaacattt gcgcaacccg tcccagaccg ttttcgagtc aaattcatcg
     1141 gacttttcct tcggtggctc ttgcggacgt ggagacgaat cagagcgatt cttcaagatc
     1201 ttgtgggagc aagtgccgtt gaacttggat gttgaactca ctctccagaa gagcgcacgc
     1261 cttctccaca gaaagttggt tgtagttgct gcattggagc aacaagccat gcgcagtcaa
     1321 gaaaaccgac atgggttgac tctcggtcaa ttcttcgaag ttcctgtcga gtcggatccg
     1381 tccttccaac gtgtccgtat caaacaaaat gaacggacgg aacttgtcat agttcccttg
     1441 aacggcagtg catcgagcca ccaactccaa aggctccaag tcatcactca gagagtactg
     1501 aggacaacct tggatctgaa gaaataggct cagatacggg atggtcagcg ctgctacttc
     1561 ttcggtgtat cggaaatccg a
//
LOCUS       DAOQUC010001097         1578 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_107167_length_1578_cov_2.235062, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001097 DAOQUC010000000
VERSION     DAOQUC010001097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107167_length_1578_cov_2.235062"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..862
                     /gene="hemB"
                     /locus_tag="PKH07_15875"
     CDS             <1..862
                     /gene="hemB"
                     /locus_tag="PKH07_15875"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HNT36466.1"
                     /translation="QESINAMPGVERLSVDLLASWSKDVLCHAVLIFGVPDENEKEED
                     ASQAIAEGGLVPQAIAAIKTARPDITVITDICLCAYTSHGHCGVLCADSSVDNDRTLE
                     MLARMSEVHAAAGADMVAPSGMMDGQVAAIRERLEKSGFHRTSIMAYAAKYASCFYGP
                     FRDAAHSAPSFGDRRSYQIAPANRRQALRDALLDEAEGADWLMVKPAMPYLDVLSELR
                     RETTLPLAAYQVSGEYAMLKQASKAGAFDERSAVLESITCIKRAGADVIITYYANDIC
                     EWLRQDADKR"
     gene            885..1526
                     /locus_tag="PKH07_15880"
     CDS             885..1526
                     /locus_tag="PKH07_15880"
                     /inference="COORDINATES: protein motif:HMM:NF018038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HNT36467.1"
                     /translation="MKGKVKTLLFTGGGIHDSKGCGEVINEILQSNEALETTWIHDDM
                     TALEKERLEPFDLIVLYYTRGEISDSQKNGLLNHIALGKGFVGVHSATASFRECPEFH
                     AMLGGLFLTHPKPRPYQVSVADPKHPITEGLVEFMVEDEQYIMDYDPRVEVLCSALWQ
                     GKAAPVVWTKTWGKGRVYYLALGHTPRDCQHEMFSVLLNRGALWAVDLLEPKS"
BASE COUNT          368 a          390 c          458 g          362 t
ORIGIN      
        1 gcaagagagc attaacgcga tgcccggggt ggagcggctg tccgttgatc tgcttgcgtc
       61 ctggtcaaag gatgttctgt gtcacgccgt actcatcttc ggtgtacccg atgagaacga
      121 gaaggaagaa gacgcttctc aggccatcgc ggaaggcggt ctcgtcccac aggcaattgc
      181 agcgatcaag acagctcgtc cggacattac ggtcatcact gacatctgtc tctgtgccta
      241 cacgagtcat ggtcactgtg gagttctgtg tgcggattca tctgttgaca atgacaggac
      301 tcttgagatg ttggcaagga tgtccgaggt tcatgccgcg gcgggcgctg acatggttgc
      361 cccgtcgggg atgatggatg gtcaagttgc ggcaattcga gagcgtcttg aaaaatcggg
      421 gtttcacagg acgtctatca tggcctatgc cgccaagtat gcctcttgtt tctacggccc
      481 ttttcgggac gcggctcatt ccgcgccgag tttcggagat cggcgttcgt atcagatcgc
      541 gccagccaac cgccgacagg cacttcgcga tgcacttctg gatgaagccg agggcgccga
      601 ttggttaatg gtcaagcctg ccatgccata tctcgatgtc cttagtgaac tgaggcggga
      661 aacaactctc ccgcttgcgg cgtaccaggt cagcggagag tatgccatgc tgaagcaggc
      721 ttcaaaggcg ggcgcattcg acgagaggtc agccgttctg gagtccatca cttgtatcaa
      781 gagggcgggc gctgacgtga ttatcaccta ctatgccaat gacatctgcg aatggcttcg
      841 ccaggatgct gataagaggt aatcaggaac aggagaaatc aggaatgaaa ggcaaagtga
      901 agacgctgtt gttcaccggt ggtggaatcc atgattccaa gggctgtggt gaggtcatca
      961 atgagattct gcagagcaat gaggctttgg agaccacatg gattcatgat gacatgaccg
     1021 ctttggagaa agagcgttta gagcctttcg atctgatagt actctactat acacgaggcg
     1081 agatttcgga ttctcagaaa aatggcctgc tgaaccatat tgcgttgggc aaaggctttg
     1141 ttggtgtgca tagcgccacc gcttcttttc gcgaatgtcc agagtttcac gccatgttgg
     1201 gtggcctgtt cctgacacac ccaaagccaa gaccctatca agtaagcgtc gccgacccga
     1261 aacatcccat cactgaaggg ttggtcgagt tcatggttga agacgagcag tacattatgg
     1321 actacgaccc gagagtcgaa gtcctttgct ctgcactgtg gcagggaaag gccgccccgg
     1381 tagtctggac aaaaacgtgg ggaaaaggaa gagtctacta tctcgctctg ggtcacacac
     1441 ccagggactg ccagcatgag atgttctcag tgctcctgaa ccgtggcgca ctttgggcag
     1501 ttgatctgct ggaaccgaaa tcctgacggt gcactgcgtc aagagtagaa ttctcgaccg
     1561 acaaacttcc attaaagc
//
LOCUS       DAOQUC010001098         1577 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_107226_length_1577_cov_3.398817, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001098 DAOQUC010000000
VERSION     DAOQUC010001098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107226_length_1577_cov_3.398817"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1330)
                     /locus_tag="PKH07_15885"
     CDS             complement(<1..1330)
                     /locus_tag="PKH07_15885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36468.1"
                     /translation="MKIFPLAMTSAFILSAIAHNAVASPLGKPGTVASYELNADPADR
                     PEYERERQLTVIASEIQIGPVVKVAGRGAFQWYGLNWTRLNGERCQLWMLMDGWWTPE
                     KTPQVLQYLWKEADWDYTLRYIHEVSGAAVLPRISLWAYGLPQNPESLGDGTPAPAMK
                     TPERIMLHGYPFKRVSVVWKREIASPSVWREIRLNPDLLLGWTSKDRDTDGRPHYRLS
                     GGYRYEPKSDEDLIAHINAGSNFFVGRPESPEISVWLVQSPVYHGSLAYKIEDWPVQL
                     YRSNYWGEMNHHDEPALGNLGRKGTPTEVAQGLQKLLNEATRPTGINGRISSRFGVGN
                     LYFEEKDNPTWEVLWETAWYQMALPNVGGLVDEDTKMEGAGVLVDLYNMAFETQIPPT
                     IENSCAIRTAVLRGAARNFNKRWGVGFYEPDSVKIRSAEIPYFVNKETTYF"
     gene            complement(1365..>1577)
                     /locus_tag="PKH07_15890"
     CDS             complement(1365..>1577)
                     /locus_tag="PKH07_15890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36469.1"
                     /translation="SSRSPTPFALFERTFKKPCGLLRDEFTLFCDAGILPAWRLLIVL
                     HFLNGEIESHFSKSAHRIRRCLTECS"
BASE COUNT          357 a          443 c          407 g          370 t
ORIGIN      
        1 agaagtaggt tgtttctttg ttgacaaagt acggtatctc cgcagagcgt atcttgaccg
       61 agtccggttc gtagaatccg acaccccatc gcttgttgaa gttgcgagcc gcgccacgca
      121 acacggcggt gcgaatggcg caggagttct ctatcgttgg gggtatctga gtttcaaacg
      181 ccatgttgta gagatcgaca agcacccccg caccttccat cttcgtgtct tcgtcaacga
      241 gaccacccac gtttggaagc gccatctgat accaagcggt ctcccacaac acttcccaag
      301 tcggattgtc cttttcttca aaatacagat tccccacacc aaagcggctg ctgatgcgcc
      361 cattgattcc ggtcggtcgg gtcgcttcgt tgagtagttt ctgcaagcct tgagccactt
      421 cggttggggt tcccttgcgt ccaagatttc caagtgccgg ttcatcgtgg tggttcatct
      481 caccccagta gtttgagcgg tatagttgca ccggccagtc ctcgatcttg taagccagac
      541 tgccgtggta gacgggactc tgaaccagcc aaacggaaat ctccggtgac tccgggcggc
      601 ccacaaagaa gttgcttccc gcattgatat gagcgattaa gtcttcatca ctcttcggct
      661 cgtaccggta cccaccggac agacgatagt gaggtcttcc atcggtatcc ctgtctttag
      721 aagtccagcc cagaagcaag tccgggttaa gtcggatttc tctccagact gaaggggaag
      781 ctatctctct cttccaaacc acggagacac gcttgaaggg gtatccatgc agcatgattc
      841 gttctggggt cttcattgcc ggtgccggtg ttccatcacc caaactctca gggttctgtg
      901 gcagcccata agcccaaagc gaaattcgcg gcagaacggc agcaccactc acctcatgga
      961 tatagcgcaa ggtgtagtcc cagtcggctt ccttccacaa atactggagc acctgtgggg
     1021 ttttctccgg cgtccaccac ccatccatca gcatccacaa ctgacagcgt tctccgttga
     1081 gtcgtgtcca gttcagaccg taccactgaa acgcccctcg tccagcaacc ttcaccaccg
     1141 gtccgatctg aatctccgag gcaatgacag tcagctggcg ctcccgttca tactcagggc
     1201 gatctgctgg gtcagcattc aactcgtatg aggcaacggt tccgggcttg cccaacggag
     1261 aggcgacagc attgtgtgca atggcagaca aaatgaatgc agatgtcatc gcaaggggga
     1321 agatcttcat aggaacctcc gaagtttgtc tgaaatccaa cagttcaaga gcattcggtc
     1381 aaacagcggc ggattcgatg tgcgctcttt gagaaatggc tctctatttc cccgttgaga
     1441 aagtggagga cgattagcaa ccgccatgcg ggcaggatgc ctgcatcaca aaacagcgtg
     1501 aactcatccc gaagtagacc gcagggtttt ttgaaggttc tctcgaagag agcgaagggt
     1561 gtagggcttc tggatga
//
LOCUS       DAOQUC010001099         1574 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_107583_length_1574_cov_3.055300, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001099 DAOQUC010000000
VERSION     DAOQUC010001099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107583_length_1574_cov_3.055300"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..409)
                     /locus_tag="PKH07_15895"
     CDS             complement(<1..409)
                     /locus_tag="PKH07_15895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36470.1"
                     /translation="MKQLKSYILLVLIVVTCFFMPLVFVPILFLFNSVNPMQLAFITD
                     FTVENRSGEDVSFTPIGTSGREGNRARLPIFAVGFPALPSLRTGNYPLRHGEQRVILY
                     DWDDINLSEIAILSQQGQMYQLVLDPTPTLRQYH"
     gene            complement(507..764)
                     /locus_tag="PKH07_15900"
     CDS             complement(507..764)
                     /locus_tag="PKH07_15900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019490355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HNT36471.1"
                     /translation="MKAKYDFNKGKRGAVLPPSGEKVRITIRLDSDIIHWFRSKVENQ
                     GGGSYQTMINDALRAHMEHQDQSLEEVLRRVVREELRTTNL"
     gene            complement(748..1014)
                     /locus_tag="PKH07_15905"
     CDS             complement(748..1014)
                     /locus_tag="PKH07_15905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004073099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HNT36472.1"
                     /translation="MFEWDRSKARENEAKQGVSFADTFAVFDDPNAMTIEDLRENEQR
                     YVTIGMDAFSRVLVVVYTWRGESIRIISARKAVRFEVKQYEGQV"
     gene            1138..1398
                     /locus_tag="PKH07_15910"
     CDS             1138..1398
                     /locus_tag="PKH07_15910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36473.1"
                     /translation="MGYASGATLYEPGMRSSAAALRSARDWKEYGQDARASSESVSPW
                     TGWRTEGLVSGASLPFWHKLPSNSFQIRLSPGKEFESIPLIY"
BASE COUNT          386 a          397 c          407 g          384 t
ORIGIN      
        1 tatggtattg gcgaagagtc ggcgtcgggt caagaacgag ctggtacatc tgcccttgtt
       61 gtgaaaggat ggcaatctcc gaaaggttaa tgtcgtccca gtcgtacagg ataactcgct
      121 gctcaccatg tctcagcggg tagttgcccg tcctgagact aggcaaggcc ggaaatccga
      181 cagcgaagat gggaagtctg gccctgttgc cctcgcgacc cgaggttcca attggagtga
      241 atgaaacatc ctcaccggag cgattctcta cagtgaagtc cgtgataaag gccaactgca
      301 tgggattcac actattgaat aagaacagaa tgggcacaaa gactaacggc atgaagaaac
      361 aagtgaccac gatcagcaca agcagtatgt aacttttgag ttgtttcatc tccgaactca
      421 acgcccctgt taagaatctg cacagcgact gcactgcatc gcgggtgaca gaatgccctc
      481 acggctgcct acagatctgc tgccttctat aggttggtcg ttcgcagttc ctcgcgcaca
      541 acgcggcgga gaacctcttc caacgactgg tcttggtgct ccatgtgggc acggagagca
      601 tcattgatca tggtctggta acttccccca ccttggttct ccaccttcga cctgaaccag
      661 tggataatgt ccgagtcaag acgaatggta atcctgacct tctcaccgga cggtggaagc
      721 accgcaccgc gtttcccttt gttgaagtca tacttggcct tcatactgtt tcacctcgaa
      781 gcgtacggct ttgcgtgccg agataattcg aatactctct ccgcgccacg tatagacaac
      841 gaccaggaca cggctgaacg cgtccattcc gatcgtaacg tagcgctgtt cgttttccct
      901 cagatcctca atcgtcatag cgtttggatc atcaaacacc gcaaaagtgt cggcaaaaga
      961 gaccccttgt ttggcctcat tctctcgtgc cttcgatctg tcccactcga acatagcact
     1021 ctatgaatat gcacagttgt atgtacatgt caagtcattc ttcaagaaag gcattcaaga
     1081 tccgtccaac aaaccaaagt gccggttagc gtgtggaatg gcaaggagtt tgcaggtttg
     1141 gggtatgctt cgggtgcaac gctgtacgag ccgggaatga gatcgtcggc agcggcgcta
     1201 cgctctgccc gcgactggaa ggaatacgga caggatgccc gcgcatccag cgagtcagtc
     1261 agcccctgga caggatggcg cactgaaggt ctggtttcag gtgcttcttt gccattttgg
     1321 cacaaactcc cctctaactc attccagatt cgtctttctc ctggaaaaga atttgaaagt
     1381 attccactaa tctattgaat caaaaagact tctgactgaa ctgtgatttg agtttgtttc
     1441 tggcacaggg gttgcttgta attggtgaga tgaagaagtc tgattgtcgt catagaggtt
     1501 gggaagctgt tcgagttgga gggaaggaag agatgatgaa gactgcaaga tgcctgacaa
     1561 cggccctgat gatg
//
LOCUS       DAOQUC010001100         1574 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_107590_length_1574_cov_2.871626, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001100 DAOQUC010000000
VERSION     DAOQUC010001100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107590_length_1574_cov_2.871626"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1574
                     /locus_tag="PKH07_15915"
     CDS             <1..>1574
                     /locus_tag="PKH07_15915"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNT36474.1"
                     /translation="DRPTRRRAGNSEPTRAMIRPSHALQERALLSLIRVFDRTSWLAV
                     LTAAFLVPVLHIPACLNPFEIPKIATLGVLICVAYGLGLPVWLFEKRWRGQSFATSLP
                     VLVLTLLAVLATVFSIHPQTSWQGSFARQNGTRTLVLFVAWYFLVRFSLCHDEGRRSK
                     MLLWAFVLAGVPMCLYGVLQHFGLDLTTGFSFGERARPHGTLAYVNLLAGYLSFLLPL
                     IGLLLLNSKRGKTRTMLCVLFVAAWYLLLATYCRGAWLAVLLFCATALLGLLFWIRRR
                     VRWRRLAVVPALMVLGTLLFYGLPWLVPQSLQESADMAGVTQRTPAQRLASSFDFGEL
                     TVASRLHMWRIALDITAEYPWFGSGPETFVFLVPTRRTLQHVEYFGRRGLVENAHNEI
                     LHLAANTGLLAVAAYLWMLIAHFLHVVKGVRMTSDSKRKTTLGALALILIAYLTRSQV
                     NVNSVENSLFFWTVLGLFAAYNVREQKPIVCSKVSSYGWKFGALAACPVTAIVLICVL
                     IRPYQAGVLGHRAAKT"
BASE COUNT          260 a          410 c          472 g          432 t
ORIGIN      
        1 cagatcgccc gacgcgccgt cgagcaggca attcagagcc gacacgtgcg atgattcgcc
       61 cttcccatgc cttgcaggag cgggctctcc tttccttgat acgggtgttt gatcgaacga
      121 gttggctggc agtgttgacg gcggcttttc tcgtacctgt cttgcatatt cccgcttgtc
      181 tgaacccttt tgagattccc aagatcgcga ctctaggtgt cctgatctgc gtggcctacg
      241 gcttgggtct tcctgtctgg ctgtttgaga aacgatggcg gggacagagt tttgccactt
      301 cccttcccgt gcttgttctc acgctgttgg cggtgctggc aacagtcttc tcgatccatc
      361 ctcagaccag ttggcagggg agttttgccc gacagaatgg aacgcggacg ctggtccttt
      421 ttgtcgcctg gtatttcctc gtacggtttt ctctctgcca tgatgaggga cggcgctcaa
      481 agatgctcct gtgggctttt gtgttggcgg gcgttcccat gtgcctctac ggagtgctcc
      541 agcattttgg cttggatctg acaacgggat tctcgtttgg cgagagggca cgtccacatg
      601 ggacactggc ctatgtgaat ctcctggcgg gatacctgtc tttcctgttg ccgttgatcg
      661 ggctgctgct tctgaactcc aaacgaggca agacacgcac catgctttgt gtcttgtttg
      721 tcgcggcatg gtatctcttg ctggcgacct attgccgcgg tgcctggctt gccgtgttgc
      781 tgttttgcgc aacagcactg ctggggctgc tgttttggat cagaaggcgg gtgcggtggc
      841 gtcgtctggc cgtcgtgccg gcactcatgg tactgggaac gcttctgttc tatgggctgc
      901 cttggctggt gcctcagagt ctacaggagt cggcagacat ggctggcgtt acccagcgaa
      961 ctccggcgca gaggcttgcg tccagttttg actttggaga gttgaccgtg gcaagccgac
     1021 tccacatgtg gcgcatcgcc ttggacatca cagcggagta tccctggttc ggaagcgggc
     1081 cggaaacatt cgtattcctg gtaccgacca gacgcacgct tcagcacgtt gagtactttg
     1141 gccgccgagg tctggttgag aatgcgcaca atgagattct tcacttggct gccaacacgg
     1201 ggcttcttgc tgttgctgct tatctttgga tgttgattgc tcatttcttg catgtcgtca
     1261 agggcgttcg gatgacttcg gacagcaaga gaaagaccac gttgggagcg ttggctctta
     1321 ttctgatagc ctatctgacg cgcagtcagg tcaatgtaaa cagtgtcgag aacagcctgt
     1381 tcttctggac ggttctgggg ctctttgcgg cgtacaatgt tcgggagcaa aagccaatag
     1441 tttgttcgaa ggtgtcctcc tatggatgga agttcggcgc tctggccgca tgtcctgtca
     1501 cggcgatagt cttgatctgt gtgctgatca gaccctatca ggctggagtg ctgggtcatc
     1561 gagctgccaa gaca
//
LOCUS       DAOQUC010001101         1573 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_107703_length_1573_cov_2.878129, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001101 DAOQUC010000000
VERSION     DAOQUC010001101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107703_length_1573_cov_2.878129"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            241..1488
                     /locus_tag="PKH07_15920"
     CDS             241..1488
                     /locus_tag="PKH07_15920"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HNT36475.1"
                     /translation="MRTFKQELELAKAEPRLRIHSFHRYFGKLIPAIPRTAVRLYASS
                     SKDVVLDPFCGSGTTLVEAKALGRDCIGFDINPLAVLVSRVKTSFHDDEMLNFQLGKL
                     MYEALRDNGKDAMAEAPFCVNRDHWYRDEVIRDLVVMKRNIEKAVDEPYREFFRACLS
                     ATNRDVSNSDPKHVFPGYSKRLRKMDANGKRVVDVFQTFEQKAKSRIRAAYEYAHYCP
                     NSSDVRVEESDARSLPTDFEQVALVVMNPPYISSIRYLESMKLEMYWLGMLESTEHQS
                     EMDKRGVGTERYGIQEYRYYHRTGLPNVDELTDRLFSQGEKKMALTVARYFEDMERVF
                     AEMARVIRPKGHLVIKISNSNVRKISVPTADLFAGSLTARHGFNLLEQFDDPFVQSRS
                     LLTKRNTYSGRMDSDFMLVLQKT"
BASE COUNT          393 a          402 c          429 g          349 t
ORIGIN      
        1 gagtgttgcg gtgtttctga accgtacgag tcgttttgag gttcaggcac ttgcccatgc
       61 tggagagaaa atgccccaga aatccacgga tttctatccc aagattccca ctggattggt
      121 attctggaaa atcgaggact tctccaaagg ctgctccaac attgccggct gacagccgca
      181 cccttccgag ggtgatacaa tgacccgaac aagacctgac agaagagagg acaacgcgag
      241 ttgagaacat tcaaacagga attggaactg gcaaaagcgg aaccaagact tcgaattcat
      301 tcatttcatc ggtatttcgg gaaactcatc ccagccatac ccagaaccgc ggtacggctg
      361 tatgcctcat cttcgaaaga tgtggttctg gatcccttct gtggctcagg cacgacgctg
      421 gttgaggcga aagcgctggg acgtgactgt atcggttttg atatcaatcc gctcgcggtg
      481 ctggtgagtc gagtgaaaac atcctttcat gacgatgaaa tgctcaactt tcagttgggc
      541 aagctcatgt atgaagcgct acgcgataac ggcaaagacg ccatggcgga agcccccttt
      601 tgtgttaatc gagatcactg gtatcgcgac gaggtgatac gggatttggt cgtgatgaag
      661 cgcaatatcg aaaaagcggt ggacgaacca taccgagagt tcttccgagc ctgtttgtca
      721 gcgaccaacc gcgatgtgtc caactccgat cccaagcatg tctttccagg ttacagcaag
      781 agactgcgga aaatggacgc gaacggcaag cgagttgtgg acgtattcca gacgtttgaa
      841 cagaaggcga agtcccgtat tcgcgcggcc tacgaatatg cccattactg tcccaactcc
      901 tccgacgttc gtgtagaaga gtccgacgcc aggagcctcc ccacggactt tgagcaagtg
      961 gcacttgtgg tcatgaatcc gccgtacatc agttccattc ggtatctgga atccatgaag
     1021 ctggaaatgt actggctcgg catgctcgag agcacggaac accagtcaga gatggacaag
     1081 cgtggtgtcg gaacggaacg ttatgggatt caggaatacc gctactatca ccggacagga
     1141 cttcccaatg tggacgagtt gaccgaccgg ctcttctccc agggagagaa gaaaatggcg
     1201 ctgaccgtgg cgcgctattt tgaggacatg gagcgggtgt ttgctgagat ggcccgtgtc
     1261 atccgaccca aagggcacct ggtcatcaag attagcaact caaatgtccg aaagatttct
     1321 gttccaacgg cggacttgtt cgcaggctct ctcaccgctc ggcatggctt caatcttctg
     1381 gagcagttcg atgatccctt tgtccagagt cgctctctgc tgaccaaacg caacacctac
     1441 agcggcagga tggacagcga tttcatgctg gttctacaga agacctagca gatcagggaa
     1501 aggctgacgg gacctagtat tcgcaactca cggtgcagtt gccgagactg acaagtccgg
     1561 actgtccctc ggt
//
LOCUS       DAOQUC010001102         1572 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_107778_length_1572_cov_3.825313, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001102 DAOQUC010000000
VERSION     DAOQUC010001102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107778_length_1572_cov_3.825313"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1202)
                     /locus_tag="PKH07_15925"
     CDS             complement(<1..1202)
                     /locus_tag="PKH07_15925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008696350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BNR-4 repeat-containing protein"
                     /protein_id="HNT36476.1"
                     /translation="MSHEVGILRVHRARRRLLFVPSAFLVAVSCSCLIGCQKTQQTSF
                     EYSEKANSKEDNQLSDTQKPPLQQGRMDGYYGIWFTLGQYQMQYGDKYSGGLGTYTAK
                     HRPLAYYAPEANKTFFVYGGTLPGKRHLLAMVSYYDHETGMVPKPMIVHDKRGVTDPH
                     DNPSICLDDKGYVWVFVSGRGRGRPGFKYRSTEPYSIEEFDLIAEQEFTYPQPVFIKG
                     RGFLHCFTKYTAGRELYWETSPDGIQWSEDQKLAGFGGHYQFTEPCGDRVATAFNWHP
                     GGSVDKRTNIYFVQTDDFGQTWKNAAGEALQMPLDAADNLALVRNFAAEKRLVYIKDL
                     RFDHDGSPVILHVVSQGSEPGPQNGLRMWSTARWVDNAWHFRDITTSDHNYDTGSLYI
                     EPDGTWKV"
     gene            complement(1275..>1572)
                     /locus_tag="PKH07_15930"
     CDS             complement(1275..>1572)
                     /locus_tag="PKH07_15930"
                     /inference="COORDINATES: protein motif:HMM:NF041540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GC-type dockerin domain-anchored protein"
                     /protein_id="HNT36477.1"
                     /translation="PSPRAPILDLNPDDWIDELDLLRFLSGWHNRVYPGNGDHNQDGV
                     VDRLDVFAFVNDWKRRNQIRADFNKDGVVDQKDALLFIEAWHTNYVRDVGEPLE"
BASE COUNT          356 a          427 c          401 g          388 t
ORIGIN      
        1 atgaccttcc acgtaccgtc cggctcaata tagagagaac ctgtgtcgta gttgtgatct
       61 gaagtggtga tgtctcggaa atgccaggcg ttgtccaccc aacgcgcagt tgaccacatt
      121 cgaagtccgt tctgtgggcc gggttcggaa ccttggctga caacgtgcag gattactgga
      181 ctgccgtcgt ggtcaaacct cagatctttg atatagacga gccgcttttc tgcggcgaaa
      241 ttcctcacca aagcaagatt atccgcagcg tcaagtggca tctggagagc ttcacctgcg
      301 gcgttcttcc acgtctgtcc gaaatcatcc gtctggacga agtagatgtt cgtacgctta
      361 tccacactcc caccagggtg ccagttgaag gcagtcgcaa cccgatcacc gcacggctca
      421 gtaaactggt agtgccctcc gaatcccgcc agtttctgat cctcgctcca ctgaatcccg
      481 tccggactgg tctcccaata gagttcccga ccggcagtat atttggtgaa gcagtggaga
      541 aatcctctcc ctttgatgaa aactggttgt ggataggtga actcctgttc tgcaatgagg
      601 tcgaattcct ctatcgagta aggttcagtg ctgcgatact taaatccagg tcggccacgt
      661 cccctgccgc tcacaaagac ccaaacatat cccttgtcat cgagacagat gctcgggttg
      721 tcgtgaggat ccgtgacgcc acgcttgtca tggacaatca tcggcttggg caccataccc
      781 gtctcatggt catagtaaga aaccatcgcc agcaaatgtc gtttgcctgg gagtgtgccg
      841 ccgtaaacaa agaaagtctt gtttgcttca ggggcatagt atgccagagg tctgtgcttg
      901 gcggtgtagg tgccaagacc accggaatac ttgtccccgt actgcatctg gtactgacca
      961 agcgtgaacc agatcccgta gtatccgtcc attctgccct gctgaagtgg gggtttctgc
     1021 gtatcgctca actggttatc ctccttggag tttgccttct ctgagtattc gaatgatgtc
     1081 tgctgcgtct tttgacaacc gatgagacag gaacaagaaa ctgcaaccag gaaggctgag
     1141 ggcacaaaga gaagtctccg acgtgcccga tgaacgcgaa ggattccgac ttcatgactc
     1201 atcaagaggc ttgttctcct gtcacagcgt tcggattctg tctgcctttg gcgacggatg
     1261 cctgatccgc attgctattc caacggttcg cccacatctc tgacgtagtt ggtgtgccat
     1321 gcctcaatga acaacagtgc atctttctga tcaacaacgc catctttgtt gaagtcagca
     1381 cgaatctggt ttctgcgctt ccagtcattg acaaaagcga agacgtccaa gcgatccacg
     1441 acaccatcct gattgtggtc tccgtttcca ggatagactc gattgtgcca acccgagagg
     1501 aacctgagga gatccagttc atcgatccag tcatcagggt tcaagtccag aatgggtgcc
     1561 cgtggagagg gc
//
LOCUS       DAOQUC010001103         1571 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_107900_length_1571_cov_3.221636, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001103 DAOQUC010000000
VERSION     DAOQUC010001103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1571
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107900_length_1571_cov_3.221636"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="PKH07_15935"
     CDS             <1..229
                     /locus_tag="PKH07_15935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HNT36478.1"
                     /translation="AFLGAFMTSYARARAECLISRCDVGLAERPERIVLILAGLLFGV
                     MKPAIVILIVVSTLTVLQRIGHTFRQLRNGK"
     gene            219..>1571
                     /locus_tag="PKH07_15940"
     CDS             219..>1571
                     /locus_tag="PKH07_15940"
                     /inference="COORDINATES: protein motif:HMM:NF021134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HNT36479.1"
                     /translation="MGNRQGTFRFPIKLAVAVFVAAFCGVVLCEYSFLRQGCFLWCVY
                     LRPLMVLGSLWLVAIGVGSRLWSLLRLPGHEADSLGFESPRVTGRVSASEFLCFATLL
                     GFGGIAYLVAALCLLQLFSPATVLVALALLAVWSAKPLEHLLEFWWSRIHIENLQPRK
                     RHRIGNRFFFQIFIPMLVLAVLVPSLLAAIQPPCQSDALRYHLAVPEVYVRHNGWVEL
                     PHSSFSNFPFMVEMIYAVAILFKSPEATGLIHFGSLLLVLALVYLLGKRLCGARPGLL
                     ATALLATSPFLPILAGWAFIEVALAAYQLAAVHALTISEERWKDSSRNSHLRLAGVLL
                     GLCLGIKYTSVFFAAFLFIWVLYLVLRKREHARNDTRQTVRSLLTFCLVAFLVGMPWY
                     LKNLILFGNPIYPLLGESLGHGNWNALNTALYKFHMSLKGGLYQTGGLDFWSRTKDLL
                     T"
BASE COUNT          279 a          414 c          428 g          450 t
ORIGIN      
        1 cgctttcctg ggagccttca tgaccagtta cgccagagca cgtgcggaat gcctgatttc
       61 gcgctgtgac gttggtctcg cggagcggcc ggagagaatc gtactgattc tagctggtct
      121 gttgttcggg gtgatgaaac cggctatcgt tatcctaatt gttgtatcaa cactcaccgt
      181 cctgcagagg ataggtcaca cgtttaggca gttacgcaat gggaaataga caaggtacat
      241 tccggttccc aatcaagttg gctgtggcgg tcttcgtggc agccttctgt ggagttgtcc
      301 tgtgcgagta ctcctttctc aggcagggtt gcttcctctg gtgcgtctac cttcgaccgc
      361 ttatggtgtt gggctcactg tggcttgttg ccattggcgt ggggagtcgc ctttggagtc
      421 ttctgcggct gccaggccat gaggcggatt cccttggttt tgagtcacca cgggtaaccg
      481 gtcgagtctc ggcatcggag tttctttgtt ttgcaacact cctgggcttc ggtgggattg
      541 cctaccttgt tgctgcgctg tgtctgcttc aactgttcag ccctgcgacg gtgcttgtgg
      601 cgttggcact tcttgcggtc tggagcgcca aacctttgga gcacctgctg gaattctggt
      661 ggtcacgaat tcacatcgaa aaccttcaac cgcgaaaacg acaccgaatc gggaatcggt
      721 ttttctttca gattttcatc cccatgcttg ttctcgcagt cctggttccg agtcttctcg
      781 cagccattca gcctccttgc cagtcggacg cgctgcgcta tcaccttgct gtccctgaag
      841 tctatgtgag acacaacgga tgggtggaac tgccacacag ttcattcagc aatttcccct
      901 tcatggtgga aatgatctat gcggtagcga tcctgttcaa gtctcctgaa gccacagggc
      961 tgattcattt cggatctctt ctgctggttt tggcactagt ctacctcttg ggcaaaaggt
     1021 tgtgcggcgc tcggcctgga ctgctcgcaa ctgcgctgtt ggccacctca ccatttctac
     1081 ccatccttgc gggttgggca tttatcgaag ttgcgttggc tgcataccaa ttggcagcgg
     1141 tacatgccct gacaatttcg gaagagcgtt ggaaagactc ctcaaggaat tctcatctgc
     1201 gtctggccgg tgttctcttg gggttgtgtc ttgggatcaa gtacacttcg gttttctttg
     1261 cagcgtttct cttcatttgg gtgctctatc ttgtcctcag aaagcgcgag cacgcgcgca
     1321 acgacactcg ccagaccgta agatcgttgc tcaccttctg tctggtggct tttctcgtcg
     1381 gaatgccctg gtatttgaag aacctgatcc tgtttgggaa ccccatctat ccgcttcttg
     1441 gtgaatctct tgggcacggc aactggaacg cactcaacac agcactctat aagtttcaca
     1501 tgagtctgaa gggcggtctt taccagacgg ggggactgga cttttggagc cgaacgaagg
     1561 acttgctcac g
//
LOCUS       DAOQUC010001104         1571 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_107903_length_1571_cov_3.085752, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001104 DAOQUC010000000
VERSION     DAOQUC010001104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1571
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107903_length_1571_cov_3.085752"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..795)
                     /locus_tag="PKH07_15945"
     CDS             complement(<1..795)
                     /locus_tag="PKH07_15945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36480.1"
                     /translation="MGQLFVAFLLLFAGAVSSGAQEISSVPADVTWEFNGDGDFEGWT
                     YSTAQMTNVFVTQGRFLATTTGTDPRLFSPASLGIDAANYPFIEIRLKATAGTGCEIF
                     FRSPTDSVYSSTKKIAFSILSNSEYVTYALDMRTVGTWTGPIHQLRFDPTNASGAVVE
                     VDYFRLITTIPTATPTNSPTQTPRPATATNTPTQTPTGPSTSTPTRTPTATFTATATN
                     TPGQIGWEFNTNGDFEGWTTLNVSSATVTGGNLQGTSSNTDPRFISA"
     gene            1280..>1571
                     /locus_tag="PKH07_15950"
     CDS             1280..>1571
                     /locus_tag="PKH07_15950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36481.1"
                     /translation="MTVRAVFLGLLGSIAVCGFTYFNDAVIYQTLLVGNHMPFSVYGA
                     LIFFLLFIRPLLERTWKKWALSGSELAVILALVLASCCIPSSGFMRTLTPTLM"
BASE COUNT          350 a          422 c          410 g          389 t
ORIGIN      
        1 ggccgaaatg aagcggggat cggtgttgga actggttccc tgcaaattgc cacctgtcac
       61 tgtcgcggaa gagacgttga gcgttgtcca accttcgaaa tcaccgttgg tattgaactc
      121 ccatccaatc tgtcctgggg tgttggttgc tgttgcggtg aatgttgccg ttggagtacg
      181 ggtcggtgtg cttgtgctgg gacccgtggg ggtctgagtt ggagtattgg tcgccgtggc
      241 gggtcttggg gtctgagtcg gcgagttggt cggcgtggca gttggaatag tcgtaatcag
      301 acgaaagtag tccacttcca caaccgcacc ggacgcattg gtcggatcaa accggagctg
      361 gtgaatgggg cccgtccaag tgcccacagt tctcatatca agggcgtaag tcacatattc
      421 tgaattgctg aggatggaga aagcgatctt cttcgtagat gagtagaccg agtctgtcgg
      481 ggagcggaag aaaatctcgc atcccgtacc cgccgttgcc ttcagtcgaa tctcgatgaa
      541 gggataattg gcagcatcaa tccccaacga cgctggcgag aaaagacgcg gatcggtgcc
      601 cgtcgtcgtg gccaggaaac gtccctgtgt aacgaagaca ttggtcatct gagctgtcga
      661 atacgtccag ccctcaaaat ccccgtctcc gttgaattcc caagtgacgt ctgccggaac
      721 agaactgatc tcctgtgccc ctgaagacac agcccccgcg aaaagcagaa ggaaagccac
      781 aaataactga cccatgagac ggattccccg ctcacggctt gacactgaaa gaccgaaaaa
      841 ctctctcatc atcatatgcc tcccgttatc gttattcttc tctaatccca aggccctgac
      901 cgcagaacgg cctcctgcac tatttcagtc tcacccaaca taaagactca agggaagttg
      961 tccacttgag attgaaacca ttcttttgtg gaagctgatt gatgctcaaa ggtctcctct
     1021 atctcaatgc cttttcaggt atcccttcaa taagccaagc gccaagccac cggccactcc
     1081 gaccggcagc attggcgccc gtttgcatat cattctgaat aacttatatc cctgtctgcc
     1141 tcagatgtca aatgtttttt ttcgcttccc tgacaattgt gtgtgatagg atgcaccgcg
     1201 gtagactttc atggtcaggt ttgccatgag gattgcgcca caaaaattgc ctttcagcaa
     1261 gtaaggactg caaagggaaa tgacagttcg cgcggtattt cttggtctgc ttggatcgat
     1321 tgcggtctgc ggcttcacct atttcaacga tgcagtcatc taccagacgc tcctggtggg
     1381 caaccacatg ccgttctccg tctacggagc actgatcttc tttctgcttt tcattcggcc
     1441 actgctggag cgaacctgga agaagtgggc actgtccggc agcgagttag ccgtcatcct
     1501 cgcactggtg cttgcgtcct gctgcattcc cagctcagga ttcatgcgaa cgctgactcc
     1561 cactctgatg c
//
LOCUS       DAOQUC010001105         1571 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_107952_length_1571_cov_2.259235, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001105 DAOQUC010000000
VERSION     DAOQUC010001105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1571
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107952_length_1571_cov_2.259235"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..338
                     /locus_tag="PKH07_15955"
     CDS             <1..338
                     /locus_tag="PKH07_15955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36482.1"
                     /translation="SAVCARFHKGTLLSLLVGGGASHWREEISFIFEQGTLSVYGNKL
                     ILQRGGGQPQEEIALEPFAHTPTRNMLDHLTKGAEILVGPEAGLRVMQLTEPAWKAAE
                     TGLPQKVKM"
     gene            521..>1571
                     /locus_tag="PKH07_15960"
     CDS             521..>1571
                     /locus_tag="PKH07_15960"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNT36483.1"
                     /translation="MKPIFTRSQKPVSVNPDSDHGKQSETQPTKDLRGEKRLALFIGL
                     IALVLNLFIWVKPHFGGFEYLTTQTSQQMHIAALARNASRGLLSVDPVVDARASVQNK
                     VLQQASAEYGLFPWLVGLVFRLFDTTDFWALFPIWICYGLLLVVGFLFARLFLSRRWS
                     IVVVVLTAIAPVVIRGSTSLLPDLPASVLVLVFLLCLARGTNHWILGVVFGFVCWLKP
                     GFFPLLIPAVWLCNRISGRRNPKSIAMAFILGWLCAVWPMIIRNILAAGNPFHSMFQL
                     KWGVLPFLVPALVLLVHTGFGSRRRTVGFFRKAFSISVLCVYVVVCLVNFPPVVGVTE
                     DEDRLTTALIDFLDGH"
BASE COUNT          288 a          435 c          424 g          424 t
ORIGIN      
        1 actccgcggt gtgtgctcga tttcacaagg gcacgctcct ttcacttctg gtgggtggtg
       61 gggcatctca ttggcgcgag gagatttctt tcatcttcga acagggaacg ctctctgttt
      121 acggcaacaa actgatcctg cagcgcggtg gaggacagcc acaggaagag attgcacttg
      181 agccttttgc acacacaccg acccggaaca tgctagacca ccttaccaag ggggctgaga
      241 ttctcgtcgg tcccgaggcg ggacttcgtg tcatgcaact gaccgagcct gcctggaagg
      301 cagccgagac cggtctgcct caaaaagtca aaatgtgact ttgttctact ccgttgcgct
      361 gtgcctgtga ctttacgggc acagcgcagc ggcaccgaaa gacgtatgca tcccatctga
      421 cagctgccta ttgcacacag ccatcctttg ccttagtatg agaatgacct ttccgcctga
      481 cgactgagga aacgcaccaa aggagttgtg agctgaaccg atgaaaccca tcttcacccg
      541 atcgcaaaaa cccgtttccg taaatcccga ttctgatcat ggcaagcagt ctgagacaca
      601 gccgacgaag gatcttcgtg gagagaagcg gcttgccttg ttcatcgggc tgatcgccct
      661 tgtgctcaac ctgtttatct gggtcaagcc gcatttcggt ggtttcgagt atctgaccac
      721 tcagacttcc caacagatgc acattgccgc tctggcgcgc aatgcctcaa ggggactgct
      781 ctcggtcgat ccagtggtgg atgccagagc ctcagtccag aacaaggttc ttcaacaggc
      841 gtctgcggag tatggactat tcccgtggct tgtgggactt gtctttcgtc tcttcgatac
      901 aactgacttc tgggcgctct ttccgatctg gatctgctat ggacttctgc ttgttgtggg
      961 ctttcttttc gcgcggctct ttctgtcacg gcggtggagc atagtcgtgg tggttctcac
     1021 ggccatcgcc cctgtcgtga ttcgaggcag tacgagtctt ctgcccgatc ttcccgcctc
     1081 cgttctggtt cttgtcttcc tcctatgtct ggcacgagga acaaatcact ggattctggg
     1141 ggttgttttc ggttttgtct gttggctcaa accggggttc ttccctttgc tgattcctgc
     1201 cgtttggctc tgcaacagga tctctggcag gcgaaaccca aaatccatcg cgatggcgtt
     1261 cattctgggg tggctctgtg ccgtctggcc gatgatcatc cgcaacattc tggccgccgg
     1321 gaatcctttc cactcgatgt tccaactgaa gtggggagtg ctccctttcc tggttcctgc
     1381 tctggtgtta cttgtccaca cgggatttgg ctccagacga cgcacagtcg gattcttccg
     1441 caaggctttt tccatctcag tgctctgtgt ctacgtggtg gtttgtctgg taaatttccc
     1501 tccggtcgtg ggggttactg aggatgagga tcgtctcacg acagcgctga ttgactttct
     1561 ggatggtcac g
//
LOCUS       DAOQUC010001106         1570 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_108023_length_1570_cov_3.069307, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001106 DAOQUC010000000
VERSION     DAOQUC010001106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1570
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108023_length_1570_cov_3.069307"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1570
                     /locus_tag="PKH07_15965"
     CDS             <1..>1570
                     /locus_tag="PKH07_15965"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HNT36484.1"
                     /translation="LGFAEVWSVAFGAELVSKAYAELIEDSRKTGKTFIATPCPAVVA
                     YIEKYMPTLHDLLAPIVSPMIATARAIRQKHGDKVKVVFIGPCIAKKEEILDPCLGGD
                     VDDVLTFREMEIMLQEKGIVLSQAALSDFDGPKSFLGRSFPISGGLLKTACIGTDILD
                     NDLVTTEGKDRVLAVLDELAEGRPSARFFDLLFCEGCINGPRMLNDRGILERKELLAR
                     FVREQSKQISLESFQKSLEEYAGVDVHRDFLHEHIPLILPSEEDITEALVQMRKLTSA
                     DHLNCGACGYPTCRENAIAVCQGIAEPGMCLPYLVEELEDLLGELQQSNQELASTQQK
                     LVQTERLASMGQLSAGVAHELNNPLGTVLIYSHMILRHLSQDDPRREDLQMIVSEANR
                     CKTIVRGLLDFARQSRVSKAPAQLTALISEVVAIMTSRADNASVRIAWDHLSSLPAMI
                     IDAAQIKQVLVNLVGNAIDATPPGGTVTITARPIQGDSTIEISVSDTGQGIPAEHLDK
                     LFTPFFTTKELGKGT"
BASE COUNT          367 a          398 c          434 g          371 t
ORIGIN      
        1 acttggtttt gccgaagtct ggagtgttgc gttcggagcg gaacttgtct ccaaagccta
       61 cgccgaactt atcgaggact cacgcaagac gggtaagacc ttcattgcaa ctccatgtcc
      121 cgcagtcgtt gcctatattg aaaagtacat gccgacattg catgatctcc ttgcgcccat
      181 cgtgtctcca atgatcgcca ctgcacgggc gattcggcag aagcacggtg acaaggtgaa
      241 agtggttttc atcggtcctt gtattgccaa gaaggaagag atccttgacc cctgtctggg
      301 gggggatgtg gacgatgttc tcacattcag agagatggag atcatgttgc aggagaaggg
      361 catcgtgctg agccaggctg cgctctcgga ttttgacggt ccaaagagtt ttcttggacg
      421 ttccttcccc atctcgggtg gcttgctgaa gaccgcctgc attgggacag atattctcga
      481 caacgatctg gttacgacag aaggaaagga tcgagtgctg gcggtgttgg atgagttggc
      541 cgaaggacgt cccagtgccc gcttcttcga tctgttgttc tgtgagggat gcatcaatgg
      601 tcccagaatg ctcaatgatc ggggcattct ggagcgaaag gagttacttg ctcgattcgt
      661 tcgtgaacaa agcaaacaga tcagtctgga gtcttttcag aagtctcttg aggaatacgc
      721 tggggttgat gtacatcgcg attttctgca tgagcatatt cccctgattc ttccttctga
      781 agaggacatc accgaggcac tggtgcagat gagaaaactc acctcagcag atcacctgaa
      841 ctgcggggct tgtggatatc caacttgtcg tgagaatgcc attgccgtct gtcagggaat
      901 tgcggagcca ggtatgtgtc tgccgtatct ggttgaggaa ctcgaagact tgttgggaga
      961 gttgcagcag tccaatcagg aacttgcctc aacacagcag aaactggttc agactgaacg
     1021 actggcctcc atgggacaac tgtcggcggg agtcgcacat gagctcaaca atcccctggg
     1081 aacagtgctg atctactccc acatgattct gcgccatctc tctcaagatg atcctcgacg
     1141 ggaagatctc cagatgattg tcagcgaggc gaaccgctgc aagaccattg ttcgaggact
     1201 tttggacttc gcaagacaat cgcgtgtctc caaagcgcca gcccaactca cagcgctcat
     1261 cagtgaagtg gttgccatca tgacctcgcg ggcggacaac gccagtgtcc ggatcgcctg
     1321 ggatcatctg agcagccttc ccgcaatgat tattgatgca gcgcagatca agcaggtgtt
     1381 ggtcaacctt gtcgggaacg ccattgacgc gacaccgcct ggggggactg taaccatcac
     1441 tgcgagacct attcagggtg acagcaccat cgaaatctcc gtgtctgata cgggtcaggg
     1501 tattcccgcg gagcacctcg acaaactctt cacgccattc tttaccacga aggaacttgg
     1561 caaaggaaca
//
LOCUS       DAOQUC010001107         1569 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_108166_length_1569_cov_2.448481, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001107 DAOQUC010000000
VERSION     DAOQUC010001107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1569)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1569)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1569
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108166_length_1569_cov_2.448481"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..753)
                     /locus_tag="PKH07_15970"
     CDS             complement(<1..753)
                     /locus_tag="PKH07_15970"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HNT36485.1"
                     /translation="MNPLLYPFVSGNTFFIGAIVLAVCVALSYLSQELLVRVALRFIA
                     SIGVIGIAFSTTVVPGLLYSLWMIVSALWFVTMSLESAARSKAAHILRGLLILFSLLA
                     IAFQIPHYRHPSLPKESFDRFYLIADSISSGINQGETTWHELIRREHSVQVVNLSRPG
                     ETLRSCLPQAKQIPPGRALVVLEIGGNDLLNRSPLSEFEKDLEELFRLVCTPERVVVL
                     FELPAPPFMTGYCRVQRTLAKRHGVKLIPRRVF"
     gene            complement(850..1356)
                     /locus_tag="PKH07_15975"
     CDS             complement(850..1356)
                     /locus_tag="PKH07_15975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36486.1"
                     /translation="MAVTSPPSVNAVDYPQAHQLEIYWLGIERTSLAPLKRLHIGTET
                     VWQSEYHYLHRTGFRDLDDLLKRIFDKDPKRSYILYKFLMDMRQNLLEVRRVLKPSRR
                     YVIVLGDTRIRGAAVQTWKYLSEIAQQVGYTAEHTFGTEVLSRDEGHSRRRKSGIERV
                     LVLRNVKD"
BASE COUNT          395 a          414 c          421 g          339 t
ORIGIN      
        1 aaacacacgc cgaggaatca gcttcactcc gtggcgtttg gcaagagtgc gttgcacacg
       61 gcagtagcct gtcataaagg gtggtgcggg aagctcaaag agaacaacga cacgttccgg
      121 tgtgcagaca agccgaaaca gttcctccaa atccttttca aattccgaca acggggatcg
      181 gttcagaaga tcgttgccac cgatttccag cacaaccagc gctcttccag gcggaatttg
      241 ctttgcctgt gggagacagc tgcgaagcgt ctctccaggt ctcgacaggt tgactacctg
      301 aacggaatgc tcacgacgga tcaactcgtg ccatgtcgtc tcaccttggt tgataccgga
      361 acttatcgag tcggcgatca gatagaatcg gtcgaaagac tctttgggaa gactggggtg
      421 ccggtaatgg ggaatctgga aggctattgc caacagcgag aacaagatca gcagtcctct
      481 gagaatgtgc gctgctttgc ttctggccgc tgattcgagg ctcatggtca caaaccagag
      541 cgccgaaacg atcatccaaa gtgaatacaa gagcccaggg acgacggttg tcgaaaacgc
      601 aatcccaatc accccgatgg atgcgatgaa acgcagagcc actcttacga gaagttcctg
      661 agaaagatag gaaagggcta cgcagactgc caggactatc gctccgatga agaatgtgtt
      721 gccactcaca aacggataca agagcggatt catgcagact ccccttcacg ctatttcact
      781 ctccagccaa agccccattc atgtgtgtca ttcctggcca gagccgagaa gcgaaaaacc
      841 agaactgaat cagtctttta cgttgcggag caccagaacg cgttcgatcc ccgatttcct
      901 tcggcgtgaa tggccttcgt cccgggatag gacctctgta ccaaaagtgt gctccgccgt
      961 atagccgact tgctgagcga tctcactaag gtatttccac gtttgaacgg cagctcctcg
     1021 gatacgagta tctccaagca caatgacata tcgtcgcgac ggtttcagca cacgccggac
     1081 ttcgagcaaa ttctgccgca tatccatcag gaatttgtag aggatgtacg agcgcttggg
     1141 atctttgtca aagattcgct tcaggaggtc atccagatcg cgaaaaccgg ttcggtgaag
     1201 atagtgatac tcactttgcc aaacggtctc ggtgccaatg tgaagccgtt ttagtggtgc
     1261 cagcgaagtg cgctcgattc caagccagta aatctccagc tgatgagcct ggggatagtc
     1321 caccgcattg acagatggcg gcgaagttac ggccaagtca aaagtattgt ccggaaatct
     1381 aattgcccgg gcatcgttgt cgtcggggat gatcgtttaa tgcccttcgg gcagcgatgc
     1441 gcgaaatcca ccagccgagg aagttgggtg tccatagcct tccgaaagct atccagaacc
     1501 aagccgggag tggtctcctg acgccggtct ttgcgggcga ccgtctggat gcactgatca
     1561 tcggcactg
//
LOCUS       DAOQUC010001108         1568 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_108239_length_1568_cov_3.234633, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001108 DAOQUC010000000
VERSION     DAOQUC010001108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1568
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108239_length_1568_cov_3.234633"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..999
                     /locus_tag="PKH07_15980"
     CDS             <1..999
                     /locus_tag="PKH07_15980"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNT36487.1"
                     /translation="IPCSSEACRPSRQETMMNTPASETPEVVALITVWAEGRPEIKVP
                     LQVGQTLTIGRDPANGLALTNDKWVSRNHCHMKCEDGKVILIDNGSANGTFVENKRLA
                     PNDPVALLIPSWFIVGHTRLLITHSEHDSDETMKAQQTRVATEGNVLVPFRKVVQERE
                     QALLVTDVVGSSIVIKDDEVLLIQVVSAIGSFLENALWSQQDSFLQCTGDGFLACFEK
                     AEPALQCAIELGEHVRSTLGVKVRLSHALHWGQVLVTTTGDVSGKNVHGVFSLERVRR
                     EDPRIKDSFETDPSRDVIVLSEPFISHLTDWDKGRAAALGTKTLKGAGDVEVFCLL"
     gene            complement(1029..>1568)
                     /locus_tag="PKH07_15985"
     CDS             complement(1029..>1568)
                     /locus_tag="PKH07_15985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36488.1"
                     /translation="RVVERGQLESILLEHELHRSDLASVENAVRVGRLLLAEHVVILR
                     VSETEDEFNAAARLVSVQSQEILWTTTFKSSSSLATESESLGRGIALSLAMLFTIQDA
                     VILDSSAWPEVYVDKGFLDGLSEGVKVVSLNGERPRLLGRISEVQDCVAKAVFSVTET
                     VFEPGQSVQVFGYMNLPKP"
BASE COUNT          380 a          456 c          377 g          355 t
ORIGIN      
        1 atcccttgtt cctcggaggc atgtcgccca tcgcggcaag aaacaatgat gaatacccct
       61 gcatctgaga ctccagaagt tgtcgctctc atcaccgtct gggctgaagg acgccccgag
      121 atcaaggttc ccctccaagt cggtcagaca ctcaccattg gacgcgatcc cgccaatggc
      181 cttgcgctta cgaacgacaa atgggtgtcg agaaatcact gccacatgaa gtgtgaagac
      241 ggaaaggtca ttctcattga caatggatcc gccaatggca cctttgtcga gaacaaacga
      301 cttgcaccca acgatccggt tgcactcttg atcccttcgt ggttcatcgt cggtcacacg
      361 agacttctga ttactcattc cgagcacgac tcggacgaga cgatgaaggc tcaacaaacc
      421 cgagtcgcca cagagggaaa cgtgctggtt cctttccgaa aagtcgtcca ggaacgagaa
      481 caagctctgc tggtaaccga cgtcgtcggt tcttcgatcg tcatcaaaga cgacgaggtt
      541 ctcttaatcc aggtcgtctc agcaatcggt tcgtttctgg aaaatgcgct ctggagtcaa
      601 caggattcgt ttcttcaatg taccggagac ggatttcttg cctgctttga gaaggcagaa
      661 cctgcattgc agtgcgcaat agaactcggc gaacatgtcc gcagcactct tggcgtcaaa
      721 gtccgcctct cgcacgcatt gcactggggg caagttctcg tgacaaccac cggagatgtc
      781 tcaggcaaga atgtgcacgg agtcttttcc cttgagagag tgcgccgaga agatccaagg
      841 atcaaagatt ccttcgaaac agatccgtcc agggatgtca ttgtcctctc ggagcctttc
      901 atctctcatc tgactgactg ggacaagggt cgcgccgctg ctttgggaac caaaaccctc
      961 aaaggtgctg gtgatgtgga agtcttctgt ctcttatgat ctgaccccga cttcattcac
     1021 ttgcagtact agggctttgg caggttcatg tatccaaaga cctgtacact ctgtcccggc
     1081 tcaaagaccg tctcggtgac actgaagacc gccttggcaa cgcagtcctg tacctcggaa
     1141 atccgaccca gcagccgcgg tcgttctcca ttgagactga caaccttaac gccttcactc
     1201 aacccatcta aaaacccctt atcaacatac acctcgggcc acgccgagct gtccaggatc
     1261 acagcgtctt gaatggtgaa tagcatcgcc agactgaggg cgatgcctcg gccaaggctc
     1321 tcgctttcag tggcaagaga actgcttgac ttgaaggttg tcgtccacag aatctcctga
     1381 ctctggacac tgacgaggcg agccgcggca ttgaattcgt cttcagtctc ggacactcga
     1441 agaatcacca catgctccgc caacaggaga cgaccaacac gaactgcatt ttccacactc
     1501 gcaagatctg atctgtgaag ttcatgctcc aataggatac tctccaactg tcccctttcg
     1561 acaacccg
//
LOCUS       DAOQUC010001109         1568 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_108243_length_1568_cov_3.152016, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001109 DAOQUC010000000
VERSION     DAOQUC010001109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1568
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108243_length_1568_cov_3.152016"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..525
                     /locus_tag="PKH07_15990"
     CDS             <1..525
                     /locus_tag="PKH07_15990"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36489.1"
                     /translation="LEPRIAYNHRNLALALWRKGDSHQAAIHYEDSLRLDPNDTDVRL
                     RLAEIYKEHDKPEQVLSHYRTVLESHPELWNIAHDLAWFIVNLKDESLRDTAEAVRLA
                     EYAVKMAPEETPVLLNTLAAAYAEASRFDDAVKSIDKSIVLARSIGREDYVVKLMSTR
                     ERYVRGESIRGKAR"
     gene            581..1441
                     /locus_tag="PKH07_15995"
     CDS             581..1441
                     /locus_tag="PKH07_15995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36490.1"
                     /translation="MRTFAVFLFCMVLLSCARNSALVPDRLPLPESRGTVAGGSALAK
                     ILSELTPDEREEEILEYVKDGKMPSFLAMLQRVTVRETVGGSERELTFFVAPDYFALG
                     SDEDYFLCPLTPATAQEIADSLACLLPTRKIVDAIYAQATVKLEPAPIAPSERMTTVP
                     VFWQHNMTVWHQRQATLALYPLGTLVAGHKKDLVITPKLAKKPTSVAIYGWHRTDGNP
                     IQPLYLGHSSSYADYSHGIRLVSRRALLDQRPVDLLDVLRDKDLCILISDEGPVSEPR
                     CPARQADGPK"
BASE COUNT          355 a          389 c          449 g          375 t
ORIGIN      
        1 cttgagcctc gcattgctta caatcacagg aatctcgcac tggcactttg gagaaagggt
       61 gattctcatc aggctgcgat ccactacgaa gactcactgc ggttggatcc caatgataca
      121 gacgtgcgct tgagacttgc cgagatctac aaagagcatg ataagccaga acaggtgttg
      181 tcacactatc gaaccgtttt ggagagccat ccagagctat ggaacattgc gcatgacctt
      241 gcctggttca tcgtcaatct gaaggatgaa agtctccgtg atacagcgga ggccgttcgt
      301 cttgcagagt atgctgtgaa gatggctccc gaggagactc cagtcttgct gaacactctg
      361 gctgcggcgt atgcggaagc aagccgattc gacgacgcag tcaaatccat tgataagtct
      421 atcgtcctgg cacgttccat cgggcgggag gattatgttg tcaaacttat gtcaacgcgt
      481 gaacgctacg tccgtgggga atctattcga gggaaggctc gatagggttg atgtctctgc
      541 gtcagggcat tcgtatccca gagagtgaag gagaatggat atgaggacgt tcgctgtgtt
      601 tctattctgc atggtgctgc tgtcttgtgc gaggaattcc gctctcgttc ctgacaggct
      661 gccgcttcca gagagccgcg gcacagtggc gggtggaagc gcgttggcga agatactttc
      721 agaactgacc cctgatgagc gtgaagagga gatccttgag tatgtcaagg acggaaagat
      781 gccttccttc ttggcgatgc ttcagcgggt gaccgttcgg gagacggttg gcggttctga
      841 acgagaactg acgttctttg tggctcctga ttactttgca ctgggctctg atgaagatta
      901 ctttctctgc cccctgacac cggctacagc acaagagatc gctgattcgc tcgcgtgcct
      961 ccttcccacc aggaagattg tggacgcgat ttatgctcag gcgactgtca aactggagcc
     1021 tgctccaatt gccccgagtg agcgcatgac gacagttccg gtcttctggc agcacaatat
     1081 gacggtctgg catcagcggc aagcaacgct cgccttgtat ccgcttggaa cacttgtggc
     1141 ggggcacaaa aaagatctgg tgatcacacc aaagttggcg aagaagccga cttcagtcgc
     1201 aatctacggg tggcatcgaa ccgatggcaa ccctattcag cctctttatc ttggtcactc
     1261 cagttcgtat gcggactaca gtcatggtat tcgactggtc agtcggcgag cgctcctgga
     1321 tcaaagaccg gttgacctgc tggatgtgct gagagacaag gatctgtgta tcttgatcag
     1381 cgatgaaggg cctgtttcag agccacgatg ccccgccagg caggctgatg gcccaaaatg
     1441 atggttcctg gcttgcgagg actcagtcca gaatatgtgt gtcatctgcc gatcaagcac
     1501 gtaagggtgt gggcgtggtt gacgcgtccg aaagtgctaa cccaatcaac agacagttca
     1561 taaggtct
//
LOCUS       DAOQUC010001110         1568 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_108260_length_1568_cov_2.773959, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001110 DAOQUC010000000
VERSION     DAOQUC010001110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1568
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108260_length_1568_cov_2.773959"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..453)
                     /locus_tag="PKH07_16000"
     CDS             complement(<1..453)
                     /locus_tag="PKH07_16000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012597081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36491.1"
                     /translation="MGVARMCAVLTTILVSLGELKAEGGGASDALPNAIKSIRVHRCD
                     VNPLIDFTTSVSLGENINGPSVIRVPSWIEKPLGNYYMYFAHHGGQYIRLAYADALQG
                     PWRIHEPGALALSQAKGFWGHIASPDVHVDTEKQEIRMYFHGPATGGQK"
     gene            complement(630..1448)
                     /locus_tag="PKH07_16005"
     CDS             complement(630..1448)
                     /locus_tag="PKH07_16005"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNT36492.1"
                     /translation="MTPSLYVTQSGDLFGRVAGRVGFSAEEILSLNPTLDPARLKVGY
                     GFFVPVRDSTEQFSSWVPTQELVCGPKGFKRIALTFDAGGDGKYAGDLSDLLERQGIR
                     CSFFITGKFAEVYPDAVRIISASGHRVHNHSYSHPDFTTISEAAMVEQVQKAESILSE
                     LTGQPIKPWFRFPFGARNAQTARIVASLGYRSIYWTLDSLDSVGEEKTSEAIVSRIVT
                     AGGMKDSDSFLDGAIVLLHLGNPRTVQAVGEIIENLRGRGFEFVTVDDLIAYRP"
BASE COUNT          384 a          450 c          385 g          349 t
ORIGIN      
        1 cttctgcccc cctgtggccg gaccgtgaaa gtacatacgg atttcctgtt tctctgtgtc
       61 aacatggacg tccggcgacg caatgtgtcc ccaaaaacct tttgcctgac tgagagccag
      121 ggcgccgggc tcgtggattc tccaaggccc ctgcagggcg tccgcgtagg caagacgtat
      181 gtactgccct ccgtgatgcg cgaagtacat gtagtaattg ccaagaggtt tctcgatcca
      241 ggaggggact ctgatcaccg agggcccgtt gatgttttca cccagagata ccgaggtcgt
      301 gaagtcgatc aacgggttca cgtcacatcg gtgtacccga atggacttga tcgcattcgg
      361 aagcgcatca gacgcgccac ctccctcagc cttcagctcg ccaagtgaaa ccagtatcgt
      421 tgtcaagact gcacacatcc tagcgactcc cattttccaa ttctcctgcc aaacaatggt
      481 tatctggatc cgcataagcg cgcaggttgc gtcatgcacc ccacacaacg acgggacgtt
      541 ctccacgtcc atagcccaat tcacatgagg aattgaactt agacgtctca ggggtgtttg
      601 tcaaagagcg tgtgtcgcgc ttctctcagt cacggcctat aggcgatcag atcgtccacc
      661 gtcacaaact caaagccacg tcccctgagg ttctcaataa tctcaccaac cgcctgaacc
      721 gttcttggat ttccaaggtg aagaaggacg atagcgccat cgagaaaact gtctgaatct
      781 ttcatcccgc cagcagtgac aatcctcgat acgattgcct ctgatgtctt ttcctctcca
      841 acgctgtcga gactgtcaag agtccagtag attgagcgat agcccagact ggcgacaatt
      901 cgggcagtct gcgcattgcg cgccccaaat ggaaaacgga accatggttt gattggctgt
      961 ccggtcagct cgctcagaat gctctctgcc ttttgaacct gttccaccat cgctgcttcc
     1021 gagatcgttg tgaagtccgg atggctgtaa gaatgattgt gtacccgatg gcctgacgcc
     1081 gagatgatcc tgacggcatc ggggtacacc tcagcaaact tgcccgtgat gaagaaggaa
     1141 catcgaatcc cttgccgctc aagcaggtca ctcaagtcac ctgcatattt cccgtcacca
     1201 cccgcgtcaa aagtcaaagc gattctcttg aaacctttgg gtccgcagac cagttcctga
     1261 gtcggtaccc atgagctgaa ttgctcagtc gagtctcgca ccggaacaaa gaaaccatat
     1321 ccgactttta gccttgcagg atcgagagtc ggattgagcg agagaatctc ctcggctgaa
     1381 aagccaactc ttcctgccac acggccaaac aaatcacccg actgggtgac ataaaggctg
     1441 ggtgtcactg aaacaatctc ctgagactgt gcccaggaga cacagaaggg aaacagaaga
     1501 agtatggaaa gtgctttgtt catgatcgct agaaaaaccg attccaagcg tcacacacga
     1561 ggcgactc
//
LOCUS       DAOQUC010001111         1567 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_108422_length_1567_cov_2.264550, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001111 DAOQUC010000000
VERSION     DAOQUC010001111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1567
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108422_length_1567_cov_2.264550"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(68..658)
                     /locus_tag="PKH07_16010"
     CDS             complement(68..658)
                     /locus_tag="PKH07_16010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36493.1"
                     /translation="MRVLRVLSILVLGVMGIFISSILLTRVVVPEMDWAVYLSEEFPL
                     LAPPNEATKSVVCGIVILLCSWMLLQSLVERRPTEQYFALAGEDGDIVFRVRTKALEE
                     FLNDAVAKLPHIGDASARIRLAEGSAYKVTLRAKAILDGLTLPQIRTSLGEQVHRDLV
                     QMLGLPAPQEIVVNVEAIKPTLAAVTKDDSATYGTF"
     gene            complement(655..1044)
                     /locus_tag="PKH07_16015"
     CDS             complement(655..1044)
                     /locus_tag="PKH07_16015"
                     /inference="COORDINATES: protein motif:HMM:NF015721.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HNT36494.1"
                     /translation="MSIYFENEFGRVTIGHSIIKQVILPEVLASKCFRPVGFQAGMDA
                     TQVPVPRSLEKAIKINDENDRLDTTILLSVLYGTPIVDESMKLKKRIRQAVESATGLK
                     VDDLILSVEKVHLDSDAKGKKQQEATK"
     gene            complement(1087..>1567)
                     /locus_tag="PKH07_16020"
     CDS             complement(1087..>1567)
                     /locus_tag="PKH07_16020"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003200233.1"
                     /note="an AccC homodimer forms the biotin carboxylase
                     subunit of the acetyl CoA carboxylase, an enzyme that
                     catalyzes the formation of malonyl-CoA, which in turn
                     controls the rate of fatty acid metabolism; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HNT36495.1"
                     /translation="RIQVEHPVTELVTGFDLIKEQIRVAAGEPLSFRQEDVRLQGWAV
                     ECRINAENVERNFMPSPGKIIRSVFPGGPGVRVDSHIYSGYIIPPNYDSMVAKLITYG
                     RSRDEAIARMRRALDEFVIEGVHTTIDLQQVILNNRDFLEGKVHTKWLESLLKVSEM"
BASE COUNT          354 a          435 c          399 g          379 t
ORIGIN      
        1 caaaggagcc ggtttctttc gcaatcgagc actggtgttg cctgcgcgtc cgcagatacg
       61 aacctgctca gaatgtgccg taggtcgcag aatcgtcttt ggtcactgca gccaatgtgg
      121 gtttgatcgc ctcaacgttg acaacgattt cttgtggcgc gggaagccca agcatctgaa
      181 ccaggtcccg atgaacctgc tcgccgagac tcgtcctgat ctgtggaagc gtcaaaccgt
      241 caaggatcgc ctttgcgcga agggtcacct tatacgcaga gccttctgca agccgaatcc
      301 gcgcgctggc atctccgatg tgcggcagct tcgccaccgc gtcattcagg aattcttcca
      361 gtgcttttgt gcgtacgcga aagacaatat ccccatcttc tcccgctaga gcaaagtatt
      421 gctccgtggg acgtcgttcg accaaactct gcagaagcat ccacgagcac agaagaatca
      481 cgatgccgca gaccacgctc ttggttgcct cgttgggagg ggcaagcaac gggaattctt
      541 cactgagata aactgcccag tccatctccg gcacaaccac tcgggtgagc aggatcgagc
      601 tgatgaagat tcccatcaca ccaagcacca gaatggagag aactcggaga actctcattt
      661 tgtcgcctct tgctgcttct tccctttggc gtcggagtcc agatgcacct tttcgacgct
      721 caggatcaga tcatccacct tcagtccggt tgcagactcg acggcctgac gtatgcgctt
      781 tttgagtttc atgctctcat ccacaatcgg agttccatag agcacactca gaaggattgt
      841 ggtgtcaagc cgatcgttct catcgttgat cttgattgct ttttccagag atcttggaac
      901 gggaacctga gtggcatcca tccccgcctg gaagcctacg ggacgaaagc atttcgaggc
      961 aagcacttcg ggaaggatga cctgcttgat gatcgaatga cctattgtga cgcgaccaaa
     1021 ctcgttttcg aaatagatgc tcatctggtt agtcctcctc ctatttggtt ctacccatgg
     1081 atattactac atttcggaaa ccttaagcaa gctttcgagc catttggtgt gaaccttgcc
     1141 ttcgagaaaa tccctgttgt tcagaatcac ctgctggaga tcgatggtcg tatgaactcc
     1201 ctcaatgacg aactcgtcaa gtgcccgacg cattcgggca atagcttcat cgcgactgcg
     1261 tccgtaagtg atgagtttgg ccaccatgga atcgtagttg ggtgggatga tatatccgct
     1321 gtaaatgtga ctgtccactc ggacaccggg cccaccggga aacacggaac ggatgatctt
     1381 gccaggagaa ggcatgaagt tacgctccac gttttcggcg ttgatacggc attcgaccgc
     1441 ccacccctga agtctcacat cttcctggcg aaacgacagc ggctctccgg cagcaacccg
     1501 aatctgttcc ttgatgagat cgaatccggt gaccagttcg gtaacgggat gttccacctg
     1561 gattcgg
//
LOCUS       DAOQUC010001112         1566 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_108474_length_1566_cov_3.735936, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001112 DAOQUC010000000
VERSION     DAOQUC010001112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1566
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108474_length_1566_cov_3.735936"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            272..628
                     /locus_tag="PKH07_16025"
     CDS             272..628
                     /locus_tag="PKH07_16025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012610272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNT36496.1"
                     /translation="MGHYALFVLFGLLIGVAASFSGLGGGFLMVPLLLYMGFTHQKAV
                     GTSFLAILVISVSALLAHNKLANVDYKIGVLLGIGGIIGAQLGPHLTQIISTSNFKKV
                     FAVILVGLAAYMFAKS"
     gene            complement(680..>1566)
                     /locus_tag="PKH07_16030"
     CDS             complement(680..>1566)
                     /locus_tag="PKH07_16030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36497.1"
                     /translation="AHRRTTAMTYGVVELGKEIVPLLEQVLSNQTFVFALFSQRYLEG
                     REKKELFDHLMLTAVLTVCIARGLDPDFRDLSLASLAMTAGLIHDMEFGDYYEKVKEG
                     LAAVQESSHAEQAAQRGAEFGLAGEIAFPVRFHHSYTTEDLQLPEVDTDIEITLQCAL
                     ATAECFVTLIDFFKNGTREAEALFTTASYAHAGRLNRESVAALGALIRSRDVLQHVDQ
                     ISAMESQCAFGCACAHPKRLGSTPTELVCNGGPDSCPHIQRSRAQLIVVDAPYSNICK
                     ERFLTLSPGAYFKCQLGS"
BASE COUNT          391 a          415 c          389 g          371 t
ORIGIN      
        1 tgatcgtact gcccctgccg gatcatcacg gcaatctctt cgggcacttc agggaagatc
       61 tgcaaattct gcgctgcaac aatggatacc atgctgacaa agacccccat cagaagaatt
      121 gctttcatga cactgacctc catatcatct tctcaatagc tatcatcgtt gatatccgag
      181 gtcaagccaa cacatcgaaa gccttgacag aattcccaaa tcagggcata ccagaccgac
      241 ctgaaaccaa ctcaaggaga agtgagaaac aatgggtcat tatgcgctgt ttgttctctt
      301 cggtttgttg ataggtgttg cggcgtcttt ttccggactg ggtggcggct tcttgatggt
      361 gccgctcctg ctgtacatgg gctttacaca ccagaaggcg gtgggaactt cgttcctggc
      421 gattctggtc atctcagtat ccgctctgct tgcacacaac aaactggcaa atgtggacta
      481 caagatcggt gtgctgttgg gtatcggcgg gattatcggt gcccagttgg ggcctcatct
      541 cacacagatt atttccacct ccaatttcaa gaaggtcttt gcggtgattc tcgtcggctt
      601 ggcggcatat atgttcgcaa agtcatagcc tgttcagaat tggagccaag gaacagatac
      661 aggcagggag tgaaggacac taagatccca actggcattt gaagtacgcc cccgggctga
      721 gggtgagaaa acgttccttg cagatattgg agtaaggggc gtcaacaacg atcaactgtg
      781 ctctgctcct ctgaatatgg gggcaactgt ccggacctcc attgcagacc agttcggtgg
      841 gtgtactccc caggcgtttc gggtgcgcac aagcgcatcc aaaggcgcat tggctttcca
      901 tggcagaaat ctggtctaca tgctgaagaa catccctgct tcggatcaac gctccaaggg
      961 cagcaacaga ttcccgattc aaacgaccag catgcgcgta actggcagtg gtaaagagcg
     1021 cttccgcttc acgggttcca ttcttgaaga agtcaatgag cgtgacaaaa cactcggctg
     1081 tagccagagc acactgcaat gtgatttcta tgtccgtgtc cacctcaggc aactgcaaat
     1141 cctctgtggt ataggagtga tggaatcgaa ccggaaaggc aatctcccca gcaaggccga
     1201 attcggctcc cctttgggct gcttgctcgg catgagatga ttcctgcacg gctgccagac
     1261 cttctttgac cttttcgtaa tagtctccaa attccatgtc atgaatcaag ccagcagtca
     1321 tcgccaggga tgccaaagac aaatcccgaa aatccggatc gagaccccgc gcaatacaaa
     1381 ctgtcaacac agcagtcagc atcagatggt cgaacaactc tttcttctct cgaccttcga
     1441 ggtatctctg ggagaaaagc gcaaaaacga atgtctggtt ggacagcacc tgttccaaca
     1501 ggggaacaat ctccttgccc aactcaacaa cgccataggt catggccgtt gttcttcggt
     1561 gcgcga
//
LOCUS       DAOQUC010001113         1566 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_108487_length_1566_cov_3.284580, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001113 DAOQUC010000000
VERSION     DAOQUC010001113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1566
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108487_length_1566_cov_3.284580"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..665)
                     /locus_tag="PKH07_16035"
     CDS             complement(<1..665)
                     /locus_tag="PKH07_16035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36498.1"
                     /translation="MRSLFPAIVVLCVVGAGCSHPEKSVRPSTTSKTSAWLSGFSVSP
                     HWSEQIKEFTTDSGVRVHINAPSAKRFRPALPTKIVYYALPNGNSIEWTIGRAKADDL
                     DWHYFIQHIGAQTRRLREVMKDCNLVVAYLEADKKSWPHWRGQREDNSELIAALVQQI
                     QDEVGVRTPKLTLSGHSGGGSFLFGFLNAFEKIPDSVERICFLDSNYGFSSEDGHGTK
                     LLE"
     gene            799..>1566
                     /locus_tag="PKH07_16040"
     CDS             799..>1566
                     /locus_tag="PKH07_16040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36499.1"
                     /translation="MNFCLCPFGGNRSPVLVVAGALLLFALGAQQAAAVDLWGDELSI
                     TLDYTYATKYMWYGYDLLWNNDGAHQPSLELGYKGFFAGIWGSWADESGHVASTELDY
                     YLGYGRTFFEECPFALDAALTYTYFDYLKVNSDGDGQEIKLLLELPNLIPLGPANLVP
                     RYEAAYEFDGKQSHEGFDDGWIQLLGLTYAIPLPDVVPCQEDMTLDVDWSIVYNDGTF
                     GSDSGWSHTTLGLSTTATWGAFYFTPAVYKQWSFEDTV"
BASE COUNT          350 a          418 c          407 g          391 t
ORIGIN      
        1 cattccaaca actttgtgcc atgcccgtct tcagaactga acccgtagtt gctgtccagg
       61 aaacaaatcc gctccaccga atctgggatc ttctcgaagg cattcagaaa accgaaaaga
      121 aaacttccgc caccactgtg tccggacaga gtcagttttg gagttcgaac gcccacttca
      181 tcctgaatct gctgcaccag agcggcaatc agctcactgt tgtcctcccg ttggccgcgc
      241 cagtgtggcc agctcttctt gtccgcttcc aggtaagcga caactagatt gcagtccttc
      301 atcacttcgc gaagtcttcg tgtctgtgcg ccaatgtgct gaatgaagta atgccagtcc
      361 aaatcatccg ccttcgctct gcctatcgtc cattcgatgg agttgccgtt gggcagggca
      421 tagtagacta tcttggtggg aagtgccggc ctgaaccgct tggcacttgg agcgttgata
      481 tgaacacgga cacccgaatc ggtggtgaac tccttgatct gttctgacca atgcgggctg
      541 acactgaatc cactcagcca tgcagaagtc ttgcttgttg tcgaggggcg tacacttttc
      601 tctggatgcg aacagcctgc gccaacgacg cagaggacca cgattgcggg aaacagagat
      661 ctcatcgtta ttctccttcc atagggcatg ttcctatagt agcacactat tgacagaacc
      721 caatggctca aatatattcg gcctgtagga ttccaaaagc actgcattga tgcgaggtct
      781 ttttccagga gggtgatcat gaatttctgc ctttgtcctt tcggaggaaa tcgttcacca
      841 gtattggttg tcgctggagc cctgcttctg ttcgcattgg gcgcacagca ggcggccgct
      901 gtggatctct ggggcgatga actgtctatc actttggact atacctatgc gacgaagtac
      961 atgtggtacg gctacgactt gctctggaat aacgatggcg cccatcagcc cagcctggaa
     1021 ctcggataca agggattctt cgcggggata tggggttcct gggcagacga aagtggacac
     1081 gttgccagca ctgagctgga ttactatctc ggctacggca ggacgttctt cgaggaatgt
     1141 cctttcgcct tggatgccgc attgacatac acctactttg actatcttaa ggtcaacagc
     1201 gacggtgatg gtcaggagat caaactgctg ctggaactcc ccaatctcat ccctcttgga
     1261 cccgcaaatc ttgttccgcg atatgaagca gcctacgagt ttgatggcaa gcaatcacac
     1321 gaaggtttcg acgatgggtg gatccagttg ctgggactta cctatgcgat tccactgccc
     1381 gacgttgtgc cctgccagga agacatgacg ctggatgtgg actggagtat cgtctacaac
     1441 gatggaacat tcggctcaga ttccggctgg agccacacga cgttgggctt gtcaaccacg
     1501 gctacctggg gagcattcta tttcacacct gctgtctaca aacagtggtc gtttgaggac
     1561 acggtc
//
LOCUS       DAOQUC010001114         1565 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_108626_length_1565_cov_2.723841, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001114 DAOQUC010000000
VERSION     DAOQUC010001114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108626_length_1565_cov_2.723841"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..119)
                     /locus_tag="PKH07_16045"
     CDS             complement(<1..119)
                     /locus_tag="PKH07_16045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015731191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance protein TerY"
                     /protein_id="HNT36500.1"
                     /translation="MRRLPVYLLLDTSGSMCGEPIEAVKNGVQTLVSALRQDP"
     gene            complement(196..828)
                     /locus_tag="PKH07_16050"
     CDS             complement(196..828)
                     /locus_tag="PKH07_16050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HNT36501.1"
                     /translation="MAITLQKGQTINLEKSQYDLSQITVGLGWHVRKEEPKGFFKSLR
                     RAGRDEDYDLDAIAFLLNKNNKVGNLGSDRLVGGDVVFFNSLKHPSGTIWLTGDNRVG
                     GQGDDDEQIIVKLESMSPNVERILFLVTIYQGRQKGQHFGMVENAFIRAVDASGKEMT
                     RFNISSEPSFNNKCAVIFAEVYRKDGGWKFRAIGDPKDTDNFVDILRQYM"
     gene            complement(843..1400)
                     /locus_tag="PKH07_16055"
     CDS             complement(843..1400)
                     /locus_tag="PKH07_16055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009963942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HNT36502.1"
                     /translation="MAISLEKGQRIDIGLSKVRVGLGWDANPADTGSEFDLDASAFML
                     NGKRKIPQEGFFVFYNNPCSPDGAVSSSGDDRTGRSSEGDDETLTVDLTKISREVQEI
                     VFVATIHDAESRKQNFGQVPNSYIRIYDAMTNEEICKYELGEDFSVETGVEFGRLYMK
                     DGRWRFEATGTGYKGGLETFLGKYA"
     gene            complement(1414..>1565)
                     /locus_tag="PKH07_16060"
     CDS             complement(1414..>1565)
                     /locus_tag="PKH07_16060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004289453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HNT36503.1"
                     /translation="ICKYELGEDFSVETCIEFGRLYKRGGQWRFEAMGVGHRNGLQEL
                     IDRYR"
BASE COUNT          348 a          490 c          324 g          403 t
ORIGIN      
        1 tacggatctt gtcgcaaggc cgacacgagc gtttggacgc cgttcttcac tgcctcaatt
       61 ggctcgccac acatagaccc tgacgtatcc aacagcagat agacaggcaa tcttctcata
      121 gttcatctcc cttgatacga caacaacgaa agtgtcggat gatcaaacag gcgtacacct
      181 gcagatatcc aacaatcaca tatactgacg caggatatcc acgaaattgt cggtgtcctt
      241 cggatcaccg atggcacgga acttccaccc tccatctttt cggtacactt ccgcgaagat
      301 cacggcacac ttgttgttaa aagacggctc cgaggagatg ttgaatcgcg tcatttcctt
      361 gccactggca tcaaccgctc ggatgaacgc gttttccacc atgccgaaat gctggccttt
      421 ctgacgccct tgatatatgg tcacgaggaa aagtattctc tccacatttg ggctcatcga
      481 ttcgagctta acgatgatct gctcgtcatc gtctccctgc cctccgacgc ggttgtctcc
      541 ggtcaaccat atggttccac tggggtgctt gaggctattg aagaacacga catctccccc
      601 tacgagccta tcactcccca ggtttcccac cttgttgttc ttattcaaca gaaatgcgat
      661 agcatccaga tcatagtcct cgtcccgccc cgctctccgc agtgatttga agaatccttt
      721 gggctcttct ttcctcacgt gccagcccaa accaaccgtg atctgggaca gatcatattg
      781 gcttttctca aggttgatcg tctgaccctt ctgcaaagtg attgccatgg ctcttacctc
      841 ctttacgcgt acttccccaa gaaagtctcc aacccgccct tgtatcctgt tccggtggct
      901 tcgaatcgcc accggccgtc tttcatatag agtcgcccga actcaacccc cgtctcaacc
      961 gagaagtctt cgcctaactc gtacttgcat atctcctcat tggtcatcgc gtcatagatt
     1021 cggatatagg aattgggcac ctgtccaaaa ttctgctttc gcgactcagc gtcatgaatt
     1081 gtcgccacaa agacgatttc ctggacctcg cgactgatct tcgtaaggtc cacagttaac
     1141 gtctcatcgt ccccttcgct gcttcgcccc gtccgatcgt cgcctgagga ggacaccgcg
     1201 ccgtcgggcg aacacggatt gttatagaat acgaagaagc cttcctgagg gattttgcgc
     1261 ttgccattga gcatgaaggc tgaagcgtcc aaatcaaact ccgatcccgt atccgcagga
     1321 ttcgcatccc aacccaagcc cacgcgaacc ttcgacagac caatgtcgat tctctggcct
     1381 ttctccaagc tgattgccat gcctgttcct tcctcacctg tacctgtcta tcagttcctg
     1441 cagtccgttt cgatggccca cgcccatagc ctcgaaacgc cactgtcccc cgcgtttgta
     1501 caaacgcccg aattcaatgc atgtctcgac agaaaagtct tcgcccaact catacttgca
     1561 tattt
//
LOCUS       DAOQUC010001115         1564 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_108714_length_1564_cov_3.499006, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001115 DAOQUC010000000
VERSION     DAOQUC010001115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1564
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108714_length_1564_cov_3.499006"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            199..>1564
                     /locus_tag="PKH07_16065"
     CDS             199..>1564
                     /locus_tag="PKH07_16065"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNT36504.1"
                     /translation="MTEDSFPKPVEVYRAHNLEGQPLPSWKQYLVKLRIYLDDRPGML
                     SCAAKLIGRCGGNITLFDYNRSEDPNMVLMDVALRDPAALQSLLEALEQEGFLRPVAA
                     TTEDLQVTDTGAVLNLVVRLQHRPGALGDFADILKQEGANVIYMRYNEARNPESADIA
                     VALSKSSLVDDLLADINEHHYHYRVVFRGVDEERVQNAIGLKLTERFFLRLKRLMPHS
                     DVDQLKQLVQSSKEISDQLLEFAHESNKDLEAGEIFTQILLFASRSLGRTGEKFTCRE
                     QAPIQVSNQVHLRTFILPTGGNIYLLSADSESIMLDCGYGLYYNDFRRYLEEHGVRPE
                     QITAIYLSHPDADHAGMSGFFQDDYGMRVVLHSKGLSAINNDNRAWGSDSPLLRLNGL
                     FTRLVNAFTRFCPPKRPEFYKEQPIGKQGELNVIDRIQFRDVTIDVVEGLGGHVPSQV
                     YFLIP"
BASE COUNT          358 a          398 c          425 g          383 t
ORIGIN      
        1 ttcggggcaa agggcatcca acagaagaga caatgaactt gccatagcgt ctgccggatg
       61 atgtggttgt ttccagagat ggggttggct atactagcaa tcaagcccac acaagtattc
      121 cagctcttgt gggaataccc agactgggcg aggcagggtc atgaatgttt tggtgtgtca
      181 cgcgaatcgg gagatacgat gactgaggat tcctttccca aacctgttga agtctatcga
      241 gcacataatc ttgaggggca accccttcct tcctggaagc agtacctggt caagttgcgc
      301 atctacctgg atgatcgtcc tggaatgctc tcctgtgcgg cgaagctcat tggccgctgt
      361 ggcgggaata tcacgctgtt cgactacaac cggtctgaag atccgaacat ggttttgatg
      421 gatgttgctc tccgcgatcc agcggctttg cagagtcttc ttgaagcgtt ggagcaggaa
      481 ggtttcctca gaccagtggc tgctacaact gaggatttgc aggtcacgga cacaggtgct
      541 gtcctgaacc tcgtggttcg acttcagcat cgccctggag ctttggggga ttttgccgac
      601 attctgaagc aggagggtgc aaacgtcatc tacatgcgtt acaacgaagc gagaaatcct
      661 gagtcggcag atattgcggt ggcactctcc aaatcctctc tggtggatga tttgttggcg
      721 gacatcaacg agcatcacta tcactaccgc gttgtctttc ggggtgtgga cgaagaacgg
      781 gtgcagaatg ccatcggact gaagttgacg gaacggttct tcctcaggct caaacggctc
      841 atgcctcatt cggatgtgga tcagctcaaa cagttggtgc aatcgagcaa agagatcagc
      901 gatcaattgc ttgagtttgc ccatgaatca aacaaagacc tggaagcggg agagatattc
      961 acgcagatcc tgttgttcgc gtctcgatct ttgggacgga caggcgagaa attcacctgt
     1021 cgggaacagg ctccgatcca agtctccaat caggtccatc tgcgcacatt catccttccg
     1081 acgggtggca acatctatct gctttcggcc gattccgaat ccattatgct ggactgcggc
     1141 tatgggcttt attacaacga tttccgacgc tacctggaag aacacggtgt cagacctgaa
     1201 cagatcaccg cgatatacct cagccatccg gatgctgacc atgccggaat gagcggcttt
     1261 ttccaggatg attacggaat gcgcgtcgtg cttcattcga aaggtctttc agccatcaat
     1321 aacgacaacc gcgcctgggg aagcgattcg ccgctcttgc gactcaatgg tctttttaca
     1381 cgccttgtga acgccttcac gcgtttttgt ccccccaagc ggcctgaatt ctacaaggag
     1441 cagcccattg gcaagcaggg tgaactgaac gtcatcgatc gaatccagtt ccgcgatgtg
     1501 acgatagacg ttgtcgaagg tcttggcggg cacgttcctt cgcaggtcta cttcctgatt
     1561 ccgg
//
LOCUS       DAOQUC010001116         1562 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_108952_length_1562_cov_3.597877, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001116 DAOQUC010000000
VERSION     DAOQUC010001116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1562
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108952_length_1562_cov_3.597877"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..818
                     /gene="hprK"
                     /locus_tag="PKH07_16070"
     CDS             <1..818
                     /gene="hprK"
                     /locus_tag="PKH07_16070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HNT36505.1"
                     /translation="FRPERIQIFGSGEMAYLEQAENSPRVLENLERIFTFNVPCVIVS
                     NGLEAPRCLCELGVKYQTPILVSPHHTTKLYKRVWEALDLVFAPRTVMHGVLVDVFGV
                     GVLIVGKGGVGKSECALELVTRGHVLVADDLVETRCLSSVVLMGYANQTIPYHMELRG
                     LGIIDVNRVFGARAIRPSKRIRLVVMLEAWDEAKEYERLGIEERYCEILEVEIPCLVI
                     PVCPGRNISTIVEVGALQQKLKRMGIDVAKEMNEKLIQLTRRKATPASGQFQK"
     gene            933..1346
                     /locus_tag="PKH07_16075"
     CDS             933..1346
                     /locus_tag="PKH07_16075"
                     /inference="COORDINATES: protein motif:HMM:NF015567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HNT36506.1"
                     /translation="MVGIIILGHAKMSFGLLEAAKEIMAIKPGVVAIPYRSQGNISEF
                     EARIKHEIANIDEGEGVLVLTDIYGATPTNVVLKFINEMHIEVVTGVNLPMVLEAITH
                     RETLGLPHLAMKVEQRSKACIVNAGEYFRCHHVAK"
BASE COUNT          354 a          385 c          464 g          359 t
ORIGIN      
        1 gctttcgccc cgaacggatc cagatattcg gctccgggga aatggcctat ctggaacagg
       61 cggaaaacag cccccgtgtt ctggagaatc tcgaacgcat ttttacgttc aatgttcctt
      121 gtgtcatcgt cagcaacggt ttggaagcac ctcgatgcct ttgtgaactg ggtgtgaaat
      181 atcaaacccc gatccttgtt tctccccacc atacgaccaa gctgtacaaa cgggtttggg
      241 aagccttgga tttggttttt gccccaagaa cagtcatgca tggcgtgttg gtggatgtat
      301 tcggggtggg cgtcctgatc gtcggtaagg ggggcgtcgg caagagcgaa tgcgctctgg
      361 agttggtgac gcgcggccat gtgctcgtgg cagacgatct ggtggaaacc cggtgccttt
      421 cttccgtcgt gttgatggga tacgccaatc agaccattcc ctatcacatg gaactgcgcg
      481 gtctggggat cattgatgtc aaccgcgtgt tcggtgcccg tgccattcgt cctagcaaac
      541 gcattcgttt ggtggtcatg ctggaagcct gggacgaggc caaggaatat gaacggctgg
      601 gcattgagga acgctactgc gagatactcg aagtagagat tccctgcctt gtgatccctg
      661 tttgtcccgg acgaaatatc agcaccatcg tcgaggtcgg agcgctccag cagaagctca
      721 agcgcatggg cattgacgtg gcgaaagaga tgaatgagaa gttgatccaa ctgacccggc
      781 gcaaggcaac gcctgcttcc gggcagtttc agaaatagag tctgaaaggg tggattgcag
      841 cccttggctt caggcaggag tcgggtgggt tctttcccta tcctgccgac tgcatgcagg
      901 ttccggagcg tgaacctgag gagcgaagag agatggtcgg aatcatcatt ctgggtcatg
      961 cgaagatgtc attcgggctt cttgaggcgg ccaaggagat tatggccatc aagccaggtg
     1021 tggtggcgat tccttatcgt tcccagggca atatctcaga attcgaagcg cggattaagc
     1081 atgagatcgc caacattgac gagggtgagg gagtccttgt tctgacggac atctacggtg
     1141 ccactccgac gaatgttgtt ctgaagttta tcaatgagat gcacatcgaa gtggtaacag
     1201 gcgtcaatct tcccatggtt ctggaagcaa ttacacatcg ggagacactg gggttgcccc
     1261 accttgccat gaaggtggaa caacgaagca aagcctgcat cgtcaatgca ggggagtact
     1321 ttcgctgtca tcacgtagcg aaatgaagac cgtcctgtcg ggacgtctga cgcatcgtgt
     1381 gcgccaacgc tctccggaca gacatatcgg acgacactgc catgcagctg tccaagacat
     1441 ttatcatccg caatgaactg ggcgtccatg cgcggcctgc cgctctgatc gtccagaaga
     1501 tgagttcgat gaagtccgac gtgatgcttg aaagcgatgg agagcgcgtc aacgctaaga
     1561 gc
//
LOCUS       DAOQUC010001117         1561 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_109073_length_1561_cov_3.804781, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001117 DAOQUC010000000
VERSION     DAOQUC010001117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1561
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109073_length_1561_cov_3.804781"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(151..924)
                     /locus_tag="PKH07_16080"
     CDS             complement(151..924)
                     /locus_tag="PKH07_16080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020383857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HNT36507.1"
                     /translation="MKLGIVTFQIAKDWNLETVIRNCEQIGLQGVELRTQHAHGVEIA
                     LAQPDRVRVKEAFAASSVQLVGLGSIFEYHYEDSETLQAHIQGTKDCILLARDVGASG
                     IKVRPNTLPEGVPEDRTLKQIGEALSECGQYGAEHGIEVRLEVHGRHTARLHRIRKIL
                     DIAAHPNVKVCWNCNPDDLADEGFDANFDRVASDISLVHLHDLDDPAYPYQHLLRRLT
                     ESGFQGYCLAEIPSSPDPLRVLRYYKALFTALLELSSRT"
     gene            complement(960..>1561)
                     /locus_tag="PKH07_16085"
     CDS             complement(960..>1561)
                     /locus_tag="PKH07_16085"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HNT36508.1"
                     /translation="CRPGVMEWEVEARARKVMYEMGAEGTSYAIWVCSGPNTRNSLCR
                     STERILQDGDLVQLTFGARYKGYCGNMCRPLTLGKPKPEYERIMTVGLQALQQTIDMM
                     APGVDSRKVFQAFDDLLSRNGMQKWALYGPAHGTGTQECEGPWLDGKKPIILEPGMAF
                     NVDIWLSDGVQGIRYEDAVIITSSGLEQLTGDKREIIIK"
BASE COUNT          368 a          431 c          386 g          376 t
ORIGIN      
        1 ccttggcttg ttgacgaagt actcagatgc cttgatgaca atctgcccct cgtcggcaga
       61 gccgagcgca gggaatagag tcagaagcag aatcactaac cagttcttgg acacatttcc
      121 accctagcat tttcgtggat gatcgcagaa tcaagtccgc gatgaaagct ccagaagcgc
      181 cgtgaaaaga gccttgtagt accgcaacac ccgcagcgga tccggactcg agggaatttc
      241 cgccaggcag tatccctgaa acccactctc agtcaacctt ctaagaagat gctgataggg
      301 ataggctgga tcatcaaggt cgtgaagatg caccaatgag atatcagagg caactctatc
      361 gaaattggcg tcgaaacctt catcagccag atcatcggga ttgcagttcc aacagacctt
      421 gacatttgga tgtgcggcga tgtcaagaat ctttctgatc ctatgcaagc gagccgtatg
      481 tctgccgtgg acttcaagtc gaacctcgat gccatgctcg gctccatact gaccgcattc
      541 gctgagcgct tctccgatct gcttgagcgt tctatcttca gggactccct ctggaagcgt
      601 atttgggcgt accttgattc cagacgcacc tacatctcgt gcaagcagaa tacagtcttt
      661 ggttccttgg atgtgcgcct gcagcgtttc ggagtcctca tagtgatact caaaaatgga
      721 accgagtccg accaattgca cactgctggc cgcaaaggcc tccttgacgc ggacgcgatc
      781 gggttgggca agagcgatct cgacgccgtg cgcatgttgt gttctcagtt caacgccctg
      841 aagtccgatt tgctcacagt tgcgaataac tgtctcaaga ttccagtcct tggctatctg
      901 aaaagtaacg attccgagtt tcatctccac agatctcttt cgtgtgggag agtcatccat
      961 cacttaatga tgatctctcg tttgtctccc gtcaactgtt caagtcccga tgaggtgatg
     1021 atgaccgcat cttcgtagcg aatgccttga acgccatcgc tcagccagat atcaacatta
     1081 aaagccatac cgggctcaag gatgataggc ttctttccat ccagccacgg tccttcacat
     1141 tcctgggttc ccgtgccatg tgccggccca tagagtgccc atttctgcat tccatttctg
     1201 gagagcagat cgtcaaacgc ctggaagact ttgcgcgaat ccacgccagg agccatcata
     1261 tcaatggtct gctggagcgc ttgaaggccg acagtcatga tgcgctcgta ttccggcttg
     1321 ggtttaccaa gggtcagcgg gcgacacatg ttgccacagt aacctttgta tcgtgctcca
     1381 aaagtgagtt gaacgagatc gccatcttga agaatgcgtt ctgttgaacg gcacagagag
     1441 ttgcgagtgt tcgggccgga gcaaacccag atggcataac ttgtaccctc agcccccatt
     1501 tcatacatga ccttgcgcgc acgggcctcg acttcccact ccatcacgcc agggcgacag
     1561 a
//
LOCUS       DAOQUC010001118         1560 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_109206_length_1560_cov_3.091030, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001118 DAOQUC010000000
VERSION     DAOQUC010001118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1560)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1560)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1560
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109206_length_1560_cov_3.091030"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1560)
                     /locus_tag="PKH07_16090"
     CDS             complement(<1..>1560)
                     /locus_tag="PKH07_16090"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HNT36509.1"
                     /translation="NSAVAHGAGIYCNDGGSPSILDCTVELNISADFGGGIFCSNSAP
                     TVTNTTFRSNEAPHGAGVYCENTSNALFTSCLIENNRAYGDLPVPQRYGAGVFCYKNA
                     SPVFRECVIRMNVAPRGGGILSQSNASPAFHNCLIVGNTADTDYGGGVCCVDSAPTFF
                     HCTLTGNVAAANGAAVRANSLSLPSLLNCVVWDNGAKSLDGPMTIRYSCVQGGFAGIG
                     NIVEDPHFVEPWDGALANLDLQLSSPCIDWATGSPLNDEPMDFEGKPRPFDIPMRGFN
                     GQGQGFDMGAYEYSEPTNTPTFTPTPTPVNFPPSLQAAVISPKPAQVGHDIVATGIGF
                     FDLEGAAPDYRYRWFINGIELIGLQQDRLTPDLFRFGEFVSVVVYPFDGQVLGEPRSD
                     SVRVTDTSFDLTVVPGSLAVLAGGRCQALVNITPRLPQNMAVALSIIAGETHSLNLSF
                     DPQIVNLRADQSGSFSILTIEAAGTAEPDHRTLFVQGNNGFNIGAIALPLEVLGAGGK
                     TLTLSADRIYV"
BASE COUNT          378 a          408 c          434 g          340 t
ORIGIN      
        1 tggacgtaga ttcggtctgc actgagcgtg agtgtctttc caccagcacc gaggacttca
       61 agaggcaaag caatggcacc gatgttgaaa ccattgttcc cttggacgaa gagtgtgcgg
      121 tgatctggtt ctgctgttcc cgctgcttca atggtcagaa tcgagaaact cccagactgg
      181 tctgcacgca ggttgacgat ctgtgggtca aaagacaggt tcagagaatg cgtttcccca
      241 gcgataattg agagggcgac cgccatgttc tgtggcagtc ttggggtgat attcaccagc
      301 gcctgacaac gaccccctgc cagcacggca agcgagcccg ggacaacagt cagatcaaag
      361 gacgtatcgg tgacgcggac tgaatctgac ctgggctcgc ccaaaacctg tccgtcgaag
      421 gggtagacaa ccacagagac aaactcccca aatcgaaaca ggtcaggtgt cagcctgtcc
      481 tgctgtagac ctatcagttc aatgccattg atgaaccagc gatagcgata gtctggagcc
      541 gcgccttcca gatcgaagaa tccaataccc gttgccacga tatcatgacc cacctgagca
      601 ggctttggcg agattacagc tgcttgcagt gatgggggga agttcacagg agtgggcgta
      661 ggtgtgaagg ttggtgtatt ggttggctct gagtactcat aggcacccat gtcaaagccc
      721 tgtccttggc cgttaaatcc gcgcatcggg atgtcaaatg gccttggctt tccttcaaaa
      781 tccatcggct catcgtttag tggagatccc gttgcccagt ctatgcatgg ggaactaagc
      841 tggagatcaa gatttgcgag agcgccgtcc caaggctcaa cgaaatgcgg atcttcaact
      901 atgttcccga tgccagcaaa gccgccttga acacaggaat atcgtatggt catggggccg
      961 tcgagactct ttgcaccgtt gtcccaaaca acacagttga gtagagaagg aagactgaga
     1021 gagtttgcgc gtaccgcggc accgttggcc gcggccacat ttccggtcaa ggtgcagtga
     1081 aagaacgttg gcgctgaatc cacgcagcag acgcccccgc cgtagtccgt gtccgccgta
     1141 ttgccaacaa tcagacaatt gtgaaacgct ggagaggcgt tcgactgact cagaattcca
     1201 cctccgcgtg gagcgacatt catcctgata acgcactctc tgaaaaccgg agaggcgttc
     1261 ttgtagcaga acactcccgc tccatagcgc tgtggcacag gaagatcgcc gtaagcccta
     1321 ttgttctcaa taagacacga ggtaaacaat gcgtttgatg tgttctcaca gtagacaccc
     1381 gcgccgtgtg gtgcttcatt ggatcggaat gtggtattcg tcaccgttgg ggcggaattt
     1441 gagcagaaaa tgccaccacc gaagtctgcg ctgatattca gttcaacggt gcagtcaaga
     1501 atactcggag agccgccgtc gttgcagtag atgcctgcac cgtgtgctac ggcagaattg
//
LOCUS       DAOQUC010001119         1559 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_109316_length_1559_cov_3.442819, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001119 DAOQUC010000000
VERSION     DAOQUC010001119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1559
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109316_length_1559_cov_3.442819"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(187..900)
                     /locus_tag="PKH07_16095"
     CDS             complement(187..900)
                     /locus_tag="PKH07_16095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020262648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNT36510.1"
                     /translation="MTNEIWVLTATAFFIGSVHTLLGPDHYLPFIVMGQARKWSLWKT
                     SWITFLCGVGHVLSSVVLGLLGVALGVAVSRLESVESTRGELAGWAFICFGLIYMVWG
                     IRRAWKNKPHSHVHLHEDGSPHQHEHTHTQAHSHVHDASEKKSITPWVLFTIFVLGPC
                     EPLIPILMYPAAQKSLFGLLWVTTVFGVTTITTMMTIVILASKGLSLVRFGRIERYAH
                     AIAGGTIFLSGMAIQVLGL"
     gene            1056..>1559
                     /locus_tag="PKH07_16100"
     CDS             1056..>1559
                     /locus_tag="PKH07_16100"
                     /inference="COORDINATES: protein motif:HMM:NF014670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HNT36511.1"
                     /translation="MQWENLTSPDFAKAAKETGVCVLTMGVIEKHGDHLPLGTDFLNV
                     HKIACLAADKEKAVVFPPFYFGQIYEARCFPGTVTIKPTLLLELIEGVLDEIGRNGFK
                     KIIIYSGHGGNKALVQFVAQCTLWKQKSYSVYLYNEKLRDDLQQKVRRIVETMMAHAC
                     EWETSISL"
BASE COUNT          385 a          409 c          417 g          348 t
ORIGIN      
        1 ggacacggtc aacgacgaaa acgattggta cgccaccttc agtgtgggat acacattttg
       61 agcaggccag cgcctgcagg cacgtgggct atgcccactc cttcggacgc ttgctcaatg
      121 aatgatccgc acctttcggg ctttgtgtca cgagaagcag gcaggaaacc gctcgggcaa
      181 tcgcccctag agtcccaaga cctgtatcgc cattccggaa aggaagatcg tcccgccagc
      241 gatggcgtgg gcgtatcttt caatgcgccc aaagcgaacg agactcaaac cctttgaagc
      301 caggatcacg atggtcatca tggtggtgat cgtcgtgacg ccaaagactg tcgtcaccca
      361 gagcaaaccg aacaggctct tttgcgcggc cggatacatg agaatcggga tgagtggttc
      421 gcagggtccc agcacgaata tcgtgaagag aacccacgga gtgatagact ttttctccga
      481 tgcatcatga acatggctgt gcgcttgtgt gtgcgtgtgt tcatgttgat gcggactgcc
      541 gtcttcgtgc aggtgcacat ggctgtgggg cttgttcttc caggctcttc ggattcccca
      601 gaccatatag atcaggccaa agcagatgaa agcccagccg gccaattcgc ctcgtgtgga
      661 ttctacagac tccaggcgtg acacagcgac cccaagcgca acgcccagca gaccgagcac
      721 cacagaactc aacacatgcc ccacaccgca cagaaaggta atccaagagg tcttccagag
      781 agaccatttt ctcgcctgtc ccatcacgat aaatggcaaa tagtggtctg gcccaagcag
      841 tgtgtgcaca gatccgatga agaacgctgt tgccgtaagg acccaaatct cgtttgtcaa
      901 agcaatctcc ttgttatgat gatttcatat ttagtatgat tgggagacta ccatcgtttc
      961 cggaaatgtg caaaggcaaa ctggggagcc cgaagggatt tgacatttca ggccccctcg
     1021 ttgatatttc tgtacacaca aggaggcacg aatcaatgca atgggaaaat ctgacatcac
     1081 ccgactttgc caaggcggcg aaggaaaccg gagtttgcgt tctgacgatg ggcgttattg
     1141 agaagcacgg ggatcatctt cctcttggaa cggacttcct caacgttcat aagattgcct
     1201 gtctggctgc ggacaaggag aaggcagtcg tcttcccacc gttctacttc gggcaaatct
     1261 atgaggctcg ctgttttcct ggcacggtta ccattaagcc gactttgctt ctggagctga
     1321 tagaaggcgt tctggatgag atcggtcgca acggattcaa aaagatcatt atctacagcg
     1381 gtcacggggg caacaaggcc ttggtgcagt ttgtcgccca gtgcacgctc tggaagcaga
     1441 agtcctattc agtctacctc tacaacgaga aattgcgcga cgatcttcag cagaaggtgc
     1501 gtcgtattgt cgagaccatg atggctcacg cctgcgaatg ggagaccagt atatcactt
//
LOCUS       DAOQUC010001120         1559 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_109340_length_1559_cov_2.727394, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001120 DAOQUC010000000
VERSION     DAOQUC010001120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1559
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109340_length_1559_cov_2.727394"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..967
                     /locus_tag="PKH07_16105"
     CDS             <1..967
                     /locus_tag="PKH07_16105"
                     /inference="COORDINATES: protein motif:HMM:NF012475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GH32 C-terminal domain-containing protein"
                     /protein_id="HNT36512.1"
                     /translation="NNLRRVVFFLLATNLGEILTLAAALVIGLDLPLTAVMVLWVKHN
                     TARIFISDDLKHWEMASDFESPGFFECPDLFELPVEGQTDSTKWIIHDASFRYCIGDF
                     DGRRFVAETELIQGEFGSNFYAAQTWNNLPGRRVQIGWMRGGVYPEMPFNQQLSFPCT
                     LTLRNTVDGLRIHRMPVAGISSLYEEKAFEGSITLNKDPRKITEGSLFDIRLETQWQA
                     DMDFCVRCYGKEIRYSAVSEQLTCMNSTATVKARDGLVDLRILVDHTSIEVFANGGLV
                     VMSFCFIPETETTPLELCSENGSLDVRSLSVFKLRSIHKHPTRSQ"
     gene            complement(1100..1522)
                     /locus_tag="PKH07_16110"
                     /pseudo
     CDS             complement(1100..1522)
                     /locus_tag="PKH07_16110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513868.1"
                     /note="acts in conjunction with GvcH to form
                     H-protein-S-aminomethyldihydrolipoyllysine from glycine;
                     forms a heterodimer with subunit 1 to form the P protein;
                     incomplete; partial in the middle of a contig; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
BASE COUNT          355 a          414 c          408 g          382 t
ORIGIN      
        1 caacaacctg cggcgggttg tgttcttcct gctggccacc aaccttggag aaatcctcac
       61 cctggcggcg gccctggtca tcggcctgga tctgcccctg accgcggtca tggtgctctg
      121 ggtcaaacac aacacggcgc gaattttcat ctcggatgat ctgaaacatt gggagatggc
      181 ttccgatttc gaatcgccgg gatttttcga gtgccctgac ctgttcgaac taccggttga
      241 aggccagacc gattccacca aatggattat tcacgacgca tccttccgat actgtatcgg
      301 tgacttcgat gggagacgat tcgtcgctga gaccgaactg attcagggag aattcggatc
      361 gaacttctat gccgcacaaa cctggaacaa cctgccaggt cgaagggttc agattggctg
      421 gatgcgcggt ggcgtgtacc ccgagatgcc attcaaccaa caactgagtt tcccttgtac
      481 gctcacactc cgaaatactg ttgacggtct tagaatccat agaatgcctg ttgcgggaat
      541 ttccagtctt tacgaggaga aagccttcga aggcagtatc acgctcaaca aggatccccg
      601 gaagataaca gaaggatccc tcttcgatat ccggttggag actcagtggc aggcagatat
      661 ggacttttgt gtcagatgct atggcaagga gattcgatac agtgcggtct cggaacagtt
      721 gacctgtatg aactcaacgg caactgtgaa agcccgagac ggattggttg atctgcgcat
      781 tctcgtggat cacacatcca ttgaagtgtt tgccaatggt ggcctggtgg tgatgtcttt
      841 ctgttttatc cccgagactg agacaactcc gctggaactg tgctcggaga acggatcact
      901 tgatgtacgt tcactctctg tcttcaaact gcgttcaatt cacaagcatc ccactcgtag
      961 tcagtaagaa tcgcagcagt gaaagaaagc ctgaaccgct gattgcgcgg atttgtggag
     1021 ttcgatgaac ttccaacctc acggagtcca cggcttcaga cacatccctg gttcagatga
     1081 tcgcggcaca ccagccagat cattccaatg acgctacgtc gggttgacga gcagcacggg
     1141 tctcatcgag tttcccaacc actgttgtct tggggcatga gatgaccgct tcaggtgcct
     1201 cctgcgccag tttcgcgatt tcgatcatcg cctcgatgaa agtatccaac gtctcctgtg
     1261 actctgtctc cgtcggctcg atcatcatcg cttccggtac aatgagcggg aagtagaccg
     1321 tcataggatg gaagcctcga tcgatgagtg ccttggcgat gttgatcgcg tggacgcctt
     1381 cttgcgcctg gcgactcgct gagaaaacac actcgtgcat acacgtttgg tcatagggaa
     1441 gatcgtaata gggtttcagc ctctccttca cgtagttggc gttcagtaca gcattccccg
     1501 aaacacgctt cagacccaaa cgtgtatgca cgagtgtgtt ttctcagcga gtcgccagg
//
LOCUS       DAOQUC010001121         1558 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_109419_length_1558_cov_3.393214, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001121 DAOQUC010000000
VERSION     DAOQUC010001121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1558
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109419_length_1558_cov_3.393214"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            367..>1558
                     /locus_tag="PKH07_16115"
     CDS             367..>1558
                     /locus_tag="PKH07_16115"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNT36513.1"
                     /translation="MNVVLITLDTLRADHLSCYGYQRQTSPNLDRFAAKGVRFKNAFC
                     TIPCTLPSHASIMTGLHPYSLSLGNNNTHLTTEVSTLAEVLKAQGYTTAAFVSSFVLA
                     PETNISQGFGVYDATNDFVVEVLDRSSNVYPGRRHALQTTRRAVEWLRNHTTATQQPF
                     FLWVHYYDPHFPYVPPPEFDRFGTFAQSGTCDLYRRLESKEISMSDVFSIESGVVSDK
                     EIERGIDLYDAAILSMDYSLGILLDALHGSLSNTIFLIVGDHGESLGEHGPVFCHFLL
                     DDPCVRVPLIVVSEGRLPQGQERDDLCSTLDILPTLLSLLRVGQSSLPDSVERSGRDL
                     FAEPIIPPRAVFADDGVKLFMVRTLFSKTLLTEDGNVMTFFDLVASPDEGRFSDPMQS
                     SHDEE"
BASE COUNT          352 a          416 c          378 g          412 t
ORIGIN      
        1 ccctcgcata actgagccag tacatgcgat caacgttgtc agatatcccc aatgcgccac
       61 aggaatatgc tgcatctaat gctcgactct cgggaaacct gacgcttcgg ttgatgggaa
      121 cccttaagca ctctttactc gcgaactcca tgcagcatag agagagacaa agaaaaatca
      181 ccgcgtccac tcggacatag gctgactctg tggtaatatc gctgatgtgg tttacctgga
      241 tagcaaaaat gatcgcgatc cgatatatgc tatcggtaca cagaacggaa cctaatgact
      301 gaattcagga aggtcctgct cgtttttatc ctctttctct tcacaacatg tgggggcaag
      361 gagccgttga atgtcgttct tatcactctc gacacactgc gcgcggatca tctctcctgc
      421 tacggttacc aacgccaaac ctctccgaac ctggatcgct ttgcagcaaa gggagtgcga
      481 ttcaagaacg ctttctgtac catcccctgc accttgccat ctcatgcttc cataatgaca
      541 ggactgcatc cctattcgct gagtttgggc aacaacaata cacatctcac aactgaagtc
      601 tcgactctgg ctgaggttct gaaggctcaa ggctatacaa ctgctgcctt tgtcagttcg
      661 ttcgtccttg ctcctgagac gaacatctcg cagggtttcg gtgtctatga cgctaccaat
      721 gattttgtcg ttgaggtctt ggatcgttcg agcaatgtct atccaggacg acgacacgcg
      781 cttcagacca cgagaagggc ggtcgaatgg ctgaggaatc ataccacggc aacccagcag
      841 ccctttttcc tctgggttca ctattacgat ccacattttc cgtacgtccc cccgcctgag
      901 ttcgatcgtt ttggaacgtt tgcccagagt ggaacctgcg acctttaccg acgtctcgaa
      961 tcaaaggaga tctccatgag tgatgtcttt tcgatcgaga gtggagtggt ctctgacaag
     1021 gagattgaga gaggaattga cctttacgat gctgcgatcc tgtctatgga ctactcgttg
     1081 ggcattcttc ttgacgctct gcatggaagc ctcagcaata cgattttcct cattgtcggc
     1141 gaccatgggg aatctcttgg cgaacatgga cccgtgtttt gtcatttcct gctggatgat
     1201 ccctgtgtga gagttccgct cattgtagtc tctgaaggcc gcctgcccca aggtcaggaa
     1261 cgcgatgatc tctgttctac gctagatatt cttcctacgc tcctgagcct gcttcgtgtg
     1321 ggacagtctt ccttgccgga cagcgttgag agaagtggca gagatttgtt cgccgagccg
     1381 attatccccc caagagcggt ttttgccgat gacggtgtca agctcttcat ggtacgcact
     1441 ctgttctcaa aaacgcttct gacagaagac gggaatgtca tgactttctt tgatctggtg
     1501 gcttcacccg atgagggcag gttctccgat ccaatgcagt ccagccatga cgaggaag
//
LOCUS       DAOQUC010001122         1555 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_109815_length_1555_cov_3.124000, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001122 DAOQUC010000000
VERSION     DAOQUC010001122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1555
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109815_length_1555_cov_3.124000"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..672
                     /locus_tag="PKH07_16120"
     CDS             <1..672
                     /locus_tag="PKH07_16120"
                     /inference="COORDINATES: protein motif:HMM:NF019344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNT36514.1"
                     /translation="YSVVLMDEIEKAHPDVFNILLQVLEEGRLTDALGHKVDFRNAVV
                     IMTSNLGTKDLTTKGSLGFQPNGDGSQTFEDMKERLMAQLKKTFNPEFLNRLDDVVVF
                     HELNREQIREIVDVLMKRLNDRMKANGIYLQPTSAATEFIAREGYDPLYGARPLKRAI
                     QHYIENPLSEQVLRGKIPRGGRIIVDVGDEGLVFIPEPLDSEQCEGKQQQVETASPPS
                     PTGAE"
     gene            764..>1555
                     /locus_tag="PKH07_16125"
     CDS             764..>1555
                     /locus_tag="PKH07_16125"
                     /inference="COORDINATES: protein motif:HMM:NF018899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HNT36515.1"
                     /translation="MRGHRIAFIVVLGIQVLVAGRLYAETLPENRVSEVTVRGLIHAS
                     EDEVRKVITVQLGDDITRRETELKIRRDIGSITALKKFRNVSVADEDVPTGKRIIYQV
                     YEYPILVDATFTGNKKLRTRTLKEEVLPEKKHMVFLSPEMEKQYVEKLREYCQKKGYS
                     NIGIQHRTEVIGEEEATLHFTIEEGSRTKITEAYIDGNYLFTHEQVFKKIRSKPSWFI
                     FTRRYNKAQMDTDLATIEQMYRDNGYLDVKATATEPEPSYKRGGVS"
BASE COUNT          418 a          369 c          439 g          329 t
ORIGIN      
        1 tattcggtcg tattgatgga tgagattgag aaggcgcatc cggatgtgtt caacattctg
       61 ctgcaggttt tggaggaagg tcgtctcacg gacgctcttg gtcacaaggt agacttccgc
      121 aatgccgttg tgatcatgac ctcgaatctg ggtaccaaag accttaccac caaaggcagt
      181 ctgggcttcc agccgaatgg ggacggttct caaacttttg aggacatgaa ggaacggcta
      241 atggctcagt tgaagaagac attcaacccg gagtttctca atcgtctgga tgacgtcgtg
      301 gtgttccatg aactgaatcg ggaacagatc agggagattg tggatgtgct gatgaagagg
      361 ctcaacgacc ggatgaaagc caatggtatc tatctgcagc caacatcagc ggcaacggaa
      421 ttcattgccc gtgaagggta cgatcccctc tatggggcgc gccccttgaa gcgagcaatt
      481 cagcattata ttgagaatcc actttccgaa caggtgctca gaggaaagat cccccgggga
      541 ggacgcatca tcgttgatgt cggtgatgag gggctggtct ttatccctga gcctttggac
      601 tcggaacagt gcgagggtaa acagcaacag gtggagacgg cgtctccacc aagccctacg
      661 ggggctgaat aaaaaaacta cttcggtcgg aatccggcac ttgggaggag aggttccggt
      721 attttcgttc cgaagggact tagggggtca ctgtctctcg aacatgaggg gtcacagaat
      781 cgcttttatt gttgtcctgg gcattcaagt cctggtggca ggtcggctct atgccgaaac
      841 gctgccggag aatcgcgttt cggaagtcac cgtgagggga ctcatccacg cttccgagga
      901 tgaagtacgc aaggtaatca cggtacaact gggtgatgac atcacccgac gtgagacgga
      961 attgaagatt cgtcgtgata ttggttcgat cactgcgttg aagaaattcc gaaatgtcag
     1021 cgttgcggat gaggacgttc ccaccggcaa gaggatcatc tatcaggtct acgagtatcc
     1081 aattcttgtg gacgccactt tcacagggaa caagaaactc agaacccgaa cactaaagga
     1141 agaagtgctt cccgaaaaga agcacatggt cttcctcagt ccggaaatgg aaaaacagta
     1201 cgttgaaaaa ctgcgggagt actgccagaa gaagggctac agcaacatag gcatccagca
     1261 ccgaaccgag gtcatcggcg aggaagaggc aacgctgcac ttcaccatag aggaaggcag
     1321 ccgaaccaaa atcaccgaag cgtatataga cgggaactac ctttttaccc atgagcaggt
     1381 tttcaagaag attcgcagca agcccagttg gtttatcttc actcggcgct acaacaaggc
     1441 gcagatggat acggacttgg cgaccataga gcagatgtat cgggacaacg gctatctcga
     1501 cgtcaaggca acagccaccg aacccgagcc ttcctacaaa cgaggcggag tctcg
//
LOCUS       DAOQUC010001123         1554 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_109932_length_1554_cov_3.119413, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001123 DAOQUC010000000
VERSION     DAOQUC010001123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109932_length_1554_cov_3.119413"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            109..714
                     /locus_tag="PKH07_16130"
     CDS             109..714
                     /locus_tag="PKH07_16130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020249238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNT36516.1"
                     /translation="MKQNETHGFTLIELLIVVAIIGILAAIAIPNFLSAQVRSKVARC
                     KEDMFSLGTALEAYAVDNNSHPYRDMSWYAFNVPALPPELRPAAAISALTTPIAFIKS
                     APLNPFMNNTDQPGGPVHYRYFGPYWKALALLGGRPNVAKVWSIGSCGPDGISNCGEW
                     GIFGQTYLEAIPGNPPLWENGCLYDPTNGVTSKGDIMRFGP"
     gene            805..>1554
                     /locus_tag="PKH07_16135"
     CDS             805..>1554
                     /locus_tag="PKH07_16135"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNT36517.1"
                     /translation="MRGLGVSSLVQKALAALLIFLALCLLLFVKTPTSRDPGRVNITV
                     WNVTGADETEPVVPNWFNESQDEIYVTAAGVPFAEIERKFLTAVIGRVPPDVFEYFGS
                     VVQWSSRGALLPLDDFIEKDGFDRSALFPALWDEMMWEGETYAIPTGAACEAFYWNKT
                     HFREAGLDPDRPPMTWDELEQFATKLTLRDSRGELSRAGYIPGYWSPWGGPMFLFWAL
                     QKGADFLSHDGRTVTLTSTACVEALEWDAGLF"
BASE COUNT          350 a          402 c          413 g          389 t
ORIGIN      
        1 aacgtacgcc aatcggaatt tgcgcccact gaacctgacc tcagttgagg agtggatcca
       61 gtactgatga cggtttcaaa acctctctac gaaaggagga tggactcgat gaaacaaaat
      121 gagactcatg gtttcactct gattgaactc ctcatcgtcg tggcaattat tggaatcctt
      181 gccgctattg ccatccccaa tttcctcagc gcacaggttc gctcaaaagt ggctcgatgc
      241 aaagaggaca tgttctctct gggcactgca ctggaagcct atgcagtgga caacaactca
      301 cacccatacc gggacatgag ctggtatgcg ttcaacgtgc ctgccttgcc accggagctg
      361 cgtcccgcag cggcaatttc cgctctgacg actcccatcg ctttcatcaa gtcagctccg
      421 ctgaatccct tcatgaacaa caccgatcag cccggtggcc ctgttcatta tcgctacttt
      481 gggccttact ggaaggcttt ggccctcttg ggtggaagac ccaacgtcgc caaagtctgg
      541 tctatcggtt cctgtggacc cgacggcatt tctaactgtg gcgaatgggg gatttttggg
      601 cagacctact tggaggcgat acccgggaat ccgcctctct gggagaatgg ctgtctgtac
      661 gacccaacca atggtgtcac cagcaagggt gatatcatgc ggtttgggcc ttaggcgatt
      721 catcacaaat gggggagagg acttcggttc tctccccctc tgcattttag gaaaccgtaa
      781 gtcaccgatc gcaacaggag cataatgcga ggtcttggag tgtcgtctct tgttcagaaa
      841 gcactagcag cattattgat attcctcgcc ttatgtctgc tgctgttcgt caagacccca
      901 acttccagag atcccggccg agtcaacata accgtctgga atgttacggg agccgatgaa
      961 acggagcccg ttgtcccaaa ctggttcaat gaatcacagg acgagattta tgtgaccgca
     1021 gcaggtgtcc cattcgccga gatagaacga aagttcctca cagccgttat cggaagagtt
     1081 cctccggacg tttttgagta ttttggttct gttgttcagt ggtcatcacg cggtgcgctg
     1141 cttcctttgg atgatttcat tgagaaggac gggtttgatc gatctgcact tttccctgcc
     1201 ttgtgggatg agatgatgtg ggagggggaa acctacgcca taccgactgg agcagcatgc
     1261 gaagcgttct actggaacaa gactcatttt cgagaggcgg gacttgatcc cgaccgtcct
     1321 ccaatgacct gggatgagct ggaacagttc gcaacaaaac tcaccctgcg tgattcccga
     1381 ggggagctgt ccagagctgg gtacattcca ggatactgga gtccttgggg cggaccgatg
     1441 ttccttttct gggcacttca gaaaggagca gattttcttt ctcatgacgg aagaacagtg
     1501 accttgacct caactgcgtg tgttgaggca cttgaatggg atgcggggct tttt
//
LOCUS       DAOQUC010001124         1553 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_110039_length_1553_cov_3.801068, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001124 DAOQUC010000000
VERSION     DAOQUC010001124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1553)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1553)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1553
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110039_length_1553_cov_3.801068"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(44..706)
                     /locus_tag="PKH07_16140"
     CDS             complement(44..706)
                     /locus_tag="PKH07_16140"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HNT36518.1"
                     /translation="MTKQAKRVLPVRNIIFDLGGVLLRWDVDWIITQVFSEPRTCELV
                     RREIFQHPDWNELDRGSLTKEQAIRHFVERTALPVSDIRKVMEAVKRCLTPLPESMEL
                     LDRLKTEGFQLYCLSNMHRASFAFIRDRFDFWSKLKGILISAYVGRVKPEPDIFEYFL
                     ERFRLNREQTVFVDDHKPNVEASQELGIRSVLFTSAAECERQIHLLARERQATGDRRK
                     TP"
     gene            822..>1553
                     /locus_tag="PKH07_16145"
     CDS             822..>1553
                     /locus_tag="PKH07_16145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36519.1"
                     /translation="MQVKRDFALKSFAYTCLVVGSVLFSFPFVWMVSSSIKVDRELFP
                     ERIQIFPGTPNAISQSPFIDRSYYEADLSEAVEPHVETLKSVLRDFEFPYPETVDREL
                     LIETIVLGLGKKLDILLPPEVWQESEATVRARVRDLLTTDMVNGLVENVYRRMLIGQL
                     RIRSYGLEEQELGKGSTFSQRLQVKTPETLTLVDAQDRGTDCALLHYDLSKGDRWILE
                     GTFDTEFDVSDLHRVQLYLRPDDNWH"
BASE COUNT          345 a          416 c          405 g          387 t
ORIGIN      
        1 agacctggaa ccgtatccgg catgatgcat tgggtacagc gtttcatgga gtcttcctcc
       61 gatctcctgt ggcctgccgt tccctggcca gcaggtgtat ctgccgttca cattcagcgg
      121 ccgaagtgaa caggacagac cgaatgccga gttcttgcga tgcctctacg ttcggcttgt
      181 ggtcgtcaac aaacactgtc tgctctcgat tgagtcgaaa gcgctcaagg aagtattcaa
      241 aaatgtcggg ctccggtttg accctcccaa cgtaggcgga gatcaaaatc cccttcagtt
      301 ttgaccagaa atcaaatcgg tctcggatga atgcaaaaga cgcgcggtgc atgttcgaga
      361 gacaatacag ttgaaatcct tccgtcttga gccgatccag cagttccatg gattcgggca
      421 gaggggtcag acacctcttg actgcttcca tgaccttccg aatgtcagaa acgggcaggg
      481 cggtgcgctc aacaaaatgc ctgatcgcct gctccttggt gaggcttccc cgatccaatt
      541 cgttccagtc tggatgttga aagatctcac ggcgcactag ttcgcacgtc cgaggctccg
      601 agaaaacctg tgtgatgatc cagtccacgt cccagcgaag caggactcca cccaagtcaa
      661 agataatgtt gcggacgggc agtactcgtt tggcttgctt cgtcaaactt ccgttctttc
      721 ctcttgagtg ttgtggctgc tgcgggtaga ctctcccagt gtctctatcg gggtcttccc
      781 gaaacagcac agcacggaac tatgaaagaa gactttggaa aatgcaagtc aagcgtgact
      841 tcgccctgaa atccttcgct tacacctgtc tggttgtggg atctgttctg ttttcgtttc
      901 ctttcgtgtg gatggtttct tccagcatca aagtggatcg ggaattgttc cccgaaagga
      961 ttcagatatt ccccggtacg cccaacgcca tatcccagtc gccctttata gaccgctcct
     1021 actacgaggc tgatctcagc gaagcggttg agcctcacgt ggagacgctc aagagcgttc
     1081 tgcgggattt tgagtttccc tatcctgaga cggtggatcg ggaattgctt atcgaaacta
     1141 tcgtgctggg tcttggcaag aaactggata ttcttctccc ccctgaagtc tggcaggaat
     1201 ccgaagcaac tgtgcgagca cgagttcgcg atctgctgac gacggatatg gtcaacggat
     1261 tggtcgagaa tgtctaccgt cgaatgctta tcgggcagct ccgcattcgt tcctatggtc
     1321 tggaagaaca ggaattgggc aagggctcaa cgttctcgca aaggctccag gtgaaaactc
     1381 ctgaaacact gacgctcgtt gacgctcagg atcgaggaac ggactgtgcg ctcctgcact
     1441 acgatctctc caaaggcgac cgatggattc tcgaaggcac ctttgataca gagttcgatg
     1501 tttccgatct ccatcgggtt cagctctatc ttcgccccga tgacaactgg cat
//
LOCUS       DAOQUC010001125         1552 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_110178_length_1552_cov_3.102204, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001125 DAOQUC010000000
VERSION     DAOQUC010001125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1552
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110178_length_1552_cov_3.102204"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..848)
                     /gene="apgM"
                     /locus_tag="PKH07_16150"
     CDS             complement(<1..848)
                     /gene="apgM"
                     /locus_tag="PKH07_16150"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HNT36520.1"
                     /translation="MLDRQKYLVLVPDGMADVPLDELDGKTPMEYAKTPNMDRLVRAA
                     EVGTTCHVPDGMTPESSVANMSIMGYDPTVYFTGRGPIEAANMGLNLEGKIAYRCNIV
                     TVKDDIMVDFSSGHITIPEGAELMRSLQESLGNDRVTFYPGRSYRHILMTGPEGADCR
                     NMPPHDIVGQNISEHLPTGQASEMLRDLMERSKDVLRNHPVNLKRIQEGKNPATQIWP
                     WGQGPRPNLPLFQDLFGLTGAVISAVDLIQGIGRLIGFRVIEVPGATGFVDTNYEGKA
                     DAALKA"
     gene            complement(858..>1552)
                     /locus_tag="PKH07_16155"
     CDS             complement(858..>1552)
                     /locus_tag="PKH07_16155"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004976.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HNT36521.1"
                     /translation="KICLLASTAFGVRIPFKDIAVEGISRITPEDLQYAAEMGYKVKL
                     LAIARDLPSSVEIRVHPTMIPKDTILASVNGVYNGVLLRSEPLGATFLYGRGAGGDPT
                     GGAVLADVIQLARNLARGTTVVPFLRPDFPSKPIKPMAEVEMSYYLRFVAINQAGVLA
                     KISAILAKHRISIASVIQKESRFLEPDMVPIVLMTYQARERDVRMALTEIERLDVIKG
                     EPLLVRVEKQLN"
BASE COUNT          325 a          446 c          416 g          365 t
ORIGIN      
        1 aatgctttga gcgcagcgtc cgcctttccc tcgtagttgg tgtcaacaaa tccggttgcc
       61 ccgggtactt cgattacccg aaaaccgatg agtcttccaa tgccctgaat caagtctacc
      121 gctgagataa ctgctccagt cagaccaaaa agatcctgaa acaaaggcag atttggtctc
      181 ggcccctgtc cccacggcca aatctgcgtc gcgggattct tgccttcctg gattcgtttc
      241 agattcaccg gatgatttcg aagtacatcc ttggaacgct ccatcaagtc ccgcaacatt
      301 tcggatgcct gccccgttgg aagatgctcg gagatgttct gaccgactat gtcatgggga
      361 ggcatattcc gacagtccgc gccctcgggg ccggtcatga ggatatgccg atagctgcgg
      421 ccgggataga atgtgacgcg atcgttgcca agggattcct gaaggctccg catcagctca
      481 gccccttcag gaatggtgat gtgccccgag ctgaaatcca ccatgatgtc atctttgact
      541 gtcacgatat tgcagcggta ggcaatcttt ccctccaggt tcagtcccat gttcgcggct
      601 tctatcggcc ccctgccggt gaagtagacc gtgggatcgt aacccatgat ggacatgttg
      661 gcgaccgagc tctcgggtgt catgccgtct ggtacgtggc atgtggttcc tacttctgcg
      721 gcacgaacca acctgtccat gttcggagtc ttggcgtact ccatcggcgt cttgccatcc
      781 agttcatcca gcggaacgtc ggccattccg tcgggcacca agacgagata cttttgtctg
      841 tccagcatca atagccctca gttcagttgt ttttcgacac gcacgagaag tggctcacct
      901 ttgatcacat ccagccgctc gatttctgtc agtgccatcc gcacatctcg ctcccgtgcc
      961 tggtaagtca tcaatacgat gggaaccatg tccggttcga gaaaacggct ctctttctga
     1021 atcaccgaag cgatgctgat gcggtgcttg gcaaggatgg ccgagatctt ggccagcaca
     1081 cctgcctggt tgattgcgac aaaacgcaga tagtaggaca tctcgacctc tgccatcggt
     1141 ttgataggct tggacgggaa gtccggtctc aggaaaggaa ccacggttgt tccgcgtgcg
     1201 aggtttcgtg ccagttgaat gacatcagcc agaaccgcgc caccggtggg atcgcccccc
     1261 gcaccgcgcc cgtagaggaa cgtggccccc agaggttcac tgcgcagcag caccccgttg
     1321 tagacgccgt tcacagatgc caggattgta tccttgggga tcatggtcgg atgaactcga
     1381 atctccacgc tgctgggaag atcacgggca atggcaagga gtttgacctt gtaacccatc
     1441 tctgcggcgt attgaagatc ttcaggtgta atgcggctga ttccctccac cgcgatatcc
     1501 ttgaagggaa ttctgacgcc gaaagccgtg gacgccagca gacatatctt gt
//
LOCUS       DAOQUC010001126         1551 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_110296_length_1551_cov_2.935160, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001126 DAOQUC010000000
VERSION     DAOQUC010001126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110296_length_1551_cov_2.935160"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..386
                     /locus_tag="PKH07_16160"
     CDS             <1..386
                     /locus_tag="PKH07_16160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36522.1"
                     /translation="TDDLKTFTVTVDDIETMGIEGTAWLVPYDLGVRQNFRILVRPTG
                     DEDVCEMIIELQRGSGQVGTWQNLNKVFLADLRKQLLGWRKLKLERMLQYITASAEIA
                     GRQLDTLGMVSDAESTNSIPAGEDS"
     gene            389..>1551
                     /locus_tag="PKH07_16165"
     CDS             389..>1551
                     /locus_tag="PKH07_16165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide transporter"
                     /protein_id="HNT36523.1"
                     /translation="MREDKEFQEFRNLLQRPEVFEEGFGWRTVIMALFVGLLMTPAQM
                     YMQLVAGIEMGPAAQWVTVILYVEVARRAFTRLKRPEIFVLFYMCGAVIHSGGGLLWH
                     QFLVQSEEMRKLGIIEYIPQWFAPSDPAVLNQRDFFMRAWLTPLGLMMLMMFITRLDH
                     FGLGYVMFRLTSDVEELPFPMAPVGAMGMTALADASSQQNTWRWRTFSIGAMAGIAFG
                     FIYLAIPNITGAIFSESLRIIPMPFVDLTQYTERWLPAMPWMVSFDVTFLITGMVLPF
                     WAMVGSFIGLIFTMVANPILHRVGILHGWERGIGAIQTIQSNTMDFYFSFSLGLSLAV
                     AAIGFIHVFSSFRRKQKETPGGGKMQWSKLFKPPADRGDIPIWVGLAIYILSTS"
BASE COUNT          342 a          402 c          424 g          383 t
ORIGIN      
        1 caacggacga tctgaagact tttactgtca cggtggacga tatcgagacc atgggcatcg
       61 aaggaaccgc atggcttgtt ccgtatgatc tgggcgtccg gcagaacttc agaatcctcg
      121 ttcgtccgac aggcgatgaa gacgtttgtg agatgatcat tgaactgcag agaggttccg
      181 gccaagtggg cacatggcag aatctgaaca aggtctttct ggcggatctg aggaagcaac
      241 tgctcggttg gcgcaaacta aaactcgaac ggatgcttca gtatataacc gcctcggcgg
      301 agatcgctgg caggcaactc gatacactcg gaatggtgag cgacgccgaa tcaacgaata
      361 gtattccagc gggtgaggac tcctagatat gcgtgaggac aaagagttcc aagagtttcg
      421 caatctgctg cagcgccccg aggtattcga ggaaggcttc ggctggcgta ctgtgatcat
      481 ggcgctcttc gtggggcttc tgatgactcc ggcacagatg tatatgcaac tggttgctgg
      541 catcgaaatg ggtcccgcag cacagtgggt taccgttatc ctctacgttg aagtggcgcg
      601 aagggcattc actcggctca aaagacctga gattttcgtc ctgttctaca tgtgtggagc
      661 cgttatccat tccggaggcg ggcttctctg gcatcaattc cttgtgcaaa gtgaggagat
      721 gcgcaaactg ggaatcattg agtacattcc acagtggttt gcccccagtg accccgccgt
      781 cctgaatcag cgggacttct tcatgagagc ctggctcaca ccacttggct tgatgatgct
      841 catgatgttc atcacacgtc tggatcactt cggtcttggc tacgtgatgt tccgcctgac
      901 ctcggacgtc gaggaacttc ccttccccat ggctcccgtt ggtgccatgg gtatgaccgc
      961 actggcagat gcttcgagcc aacaaaacac atggcgctgg cgcacattca gtatcggcgc
     1021 catggcgggc attgccttcg ggtttatcta cctcgccatt ccaaacatca caggggctat
     1081 cttctcggaa tcacttcgca tcatcccgat gccatttgtg gatttgactc agtataccga
     1141 acgctggttg cccgcaatgc catggatggt gagttttgat gtaacgtttc tcatcacggg
     1201 catggttctt ccgttctggg caatggtagg cagttttatc ggtctgattt tcaccatggt
     1261 cgccaatccc attttgcatc gcgtcggaat ccttcatggc tgggagagag gcatcggcgc
     1321 aattcagaca atccagtcca acacgatgga tttctatttc agtttcagtc tggggctgag
     1381 ccttgccgtc gccgcgatcg gtttcatcca tgtcttcagc agttttaggc gaaagcaaaa
     1441 ggaaacccca ggcggtggga agatgcagtg gagcaaactc ttcaagcccc ccgcagaccg
     1501 tggagacatt ccgatctggg taggactcgc gatatatata ctctccacat c
//
LOCUS       DAOQUC010001127         1547 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_110690_length_1547_cov_4.169571, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001127 DAOQUC010000000
VERSION     DAOQUC010001127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1547
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110690_length_1547_cov_4.169571"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1267
                     /locus_tag="PKH07_16170"
     CDS             <1..1267
                     /locus_tag="PKH07_16170"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNT36524.1"
                     /translation="TLQFIDPLLEEGHIVHLVAMRITGLEESFQREVRVQKGNLTYIS
                     VDEVACFRSDEYLQCQHDEFQPDCIIGVNAFPSTRAVQIRTQCPVWADINGYSMGEAQ
                     TKAYREGDDLYVGHNWNQQKPALERADKFSSASDLQRHILVGELGTVGRLNRYTMGYN
                     FVHTVPEGRKETPIVLTDNNPRWTEFRPEDFLVLWSGGYNLWCDVDTMFAGLERAMAQ
                     ESRIHFVSTGGIIDGVDEKTYPHFQEMVSRSQFSDRFHLKGWIPFEQVPDYFLCSDIG
                     INVDAQCYESMYGARNRITDMMRAGLPILTTLGTEISRLVGEAQCGLTFPIGNAEALS
                     EALLYAAHHSDEMRLMGNAGKDYFQKYYTFRETIKPVLQWVSSPSHAPDWGKPKPLQE
                     PPQPVTSVPTSQTFWHRVLSKFKEKGYWA"
     gene            1264..>1547
                     /locus_tag="PKH07_16175"
     CDS             1264..>1547
                     /locus_tag="PKH07_16175"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-guanine transglycosylase"
                     /protein_id="HNT36525.1"
                     /translation="MTRIFDFNLVHRDGDARVGRILTSHGVVETPVFMPVGTQATVKA
                     MTPEELTQIGSTMILANTYHLHLRPGEQVVHAAGGLHSFMNWNGAILTDSG"
BASE COUNT          378 a          427 c          410 g          332 t
ORIGIN      
        1 gacccttcag ttcattgatc cacttcttga agagggtcat atcgtccact tggttgccat
       61 gcggattacc ggacttgagg agtccttcca acgagaagtc agagtccaaa aggggaatct
      121 aacctatatt tcagtggacg aggtcgcctg ttttcgtagc gatgaatacc ttcagtgtca
      181 gcatgatgag tttcagcccg actgcatcat cggggtcaac gcctttccaa gcacaagagc
      241 cgtgcaaatc agaacgcagt gtcccgtctg ggcggacatc aacggctaca gcatggggga
      301 agcccagacc aaggcctatc gcgagggaga cgatctttac gtcggccaca actggaatca
      361 acagaaacct gcgctggaac gtgcagacaa gttctcctcg gcttctgact tgcagcgtca
      421 tattctcgtc ggagaacttg gcacagtggg acggctcaac cgctacacga tgggatacaa
      481 cttcgtccac actgttcccg aaggcagaaa agagacaccg atcgtgctca cagataacaa
      541 tcctcggtgg actgagtttc gcccagagga cttccttgtc ctctggagcg gtggctacaa
      601 cctctggtgc gacgtagaca caatgtttgc cggactcgaa agagcgatgg cgcaagaatc
      661 tcgaatccat tttgtgtcaa ccggaggcat cattgacgga gtggatgaga agacctaccc
      721 gcactttcag gaaatggtat ctcgctcaca gttctcggat cgctttcatc tgaaaggctg
      781 gattcccttt gaacaggtgc cggactactt cctctgctcg gacatcggga tcaacgtgga
      841 tgcacagtgc tacgagtcca tgtatggcgc acgcaaccga atcacagata tgatgcgtgc
      901 cgggcttccg atcctgacca cgctcggtac cgaaatcagc cgtcttgtgg gagaagcgca
      961 atgtggtctg acttttccca tcggcaacgc cgaggctctt tccgaggcgc tgctctatgc
     1021 ggcgcaccat tccgatgaga tgagactaat gggcaacgcc ggcaaagact actttcagaa
     1081 atactacaca ttccgagaga cgatcaagcc ggtactccaa tgggtctcca gcccttctca
     1141 cgcaccggac tggggcaagc ccaagcctct ccaggaacca ccacagccag taacatcggt
     1201 gccaacgtcg caaaccttct ggcatcgagt cctctccaag ttcaaggaga aggggtattg
     1261 ggcatgacga ggatattcga cttcaatctg gttcatcgcg atggcgatgc tcgcgtcggt
     1321 cgaattctga catctcacgg agtcgtcgaa acccctgtgt ttatgccggt aggaactcag
     1381 gcaaccgtca aggcgatgac tcctgaggaa ctgacgcaga tcgggtcaac gatgatcctc
     1441 gcaaacacct atcacctgca tctgcgcccg ggagaacaag tggtgcatgc agcgggagga
     1501 ctgcattcat tcatgaactg gaatggtgcc attctaaccg acagcgg
//
LOCUS       DAOQUC010001128         1547 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_110754_length_1547_cov_2.280831, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001128 DAOQUC010000000
VERSION     DAOQUC010001128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1547
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110754_length_1547_cov_2.280831"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..869
                     /locus_tag="PKH07_16180"
     CDS             <1..869
                     /locus_tag="PKH07_16180"
                     /inference="COORDINATES: protein motif:HMM:NF024976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HNT36526.1"
                     /translation="MDGKPVMLVECKWCGTDLDQAEMSQLFRYFAVVEARIGILTNGI
                     IYRFYTDLEEPNKMDKKPFFEVNLLDVQEQSVAELKKLSRSSFNLQEVLSAACDLKYT
                     REIKRILADEMAQPSDDFARFFAERIHSGRITQNVKAQFVEITRKAFRSFLNDQITER
                     LKSAMATPEPPAGEPVAPDAVESEPVPESRVVTTEEELEAFYLVKSILRNVVDPSRIT
                     YRDYINACSVLLDENKRKVVVQFVFNERGKQIRFLTEDRTDYDKYPIEDLNDIYKHAD
                     RIIAEARKYEAK"
     gene            complement(943..1018)
                     /locus_tag="PKH07_16185"
     tRNA            complement(943..1018)
                     /locus_tag="PKH07_16185"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(983..985),aa:Ala,seq:ggc)
     gene            complement(1091..>1547)
                     /locus_tag="PKH07_16190"
     CDS             complement(1091..>1547)
                     /locus_tag="PKH07_16190"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HNT36527.1"
                     /translation="AEAWKRTAALLSEIGGKLLKEGIQLSYHNHNHEFKLFGAKHGLD
                     ILYENSDPKALHAEIDTYWVQYAGVDPATYMKKVANRLSLVHIKDMADDDMKSFAEIG
                     NGILDWDAIFAVAKEAGSKWLIVEQDSCKRPPLESAKISFENLKTMGAV"
BASE COUNT          375 a          424 c          415 g          333 t
ORIGIN      
        1 tgatggatgg gaagcctgtc atgctcgtgg agtgcaaatg gtgcggcaca gacctcgatc
       61 aggcagagat gtcccagctg ttcaggtact tcgccgtagt cgaggcacgt atcggcatcc
      121 tcacgaacgg tattatttac aggttctaca ccgatttgga ggaaccgaac aagatggaca
      181 agaagccttt ctttgaggtg aacctccttg acgtgcaaga gcaatcggtg gcagaactca
      241 agaaattgag tcgatcgtcg ttcaaccttc aggaggttct ctccgccgct tgcgacctca
      301 aatacacgag agagatcaag cggattcttg cggacgagat ggcgcaaccg tctgacgatt
      361 tcgcgaggtt cttcgccgag cggattcatt cgggcaggat cacgcagaac gtcaaagcgc
      421 agttcgtgga gatcaccaga aaggcgttcc gcagtttctt gaatgaccag atcacagaac
      481 gtctcaaatc cgccatggcg acccccgaac cccctgccgg cgaaccagtg gcgccggatg
      541 ccgtcgagtc ggagccagtc ccggagtccc gggttgtcac tacggaagaa gagttagaag
      601 cgttctacct cgtcaaatcg attttgcgaa acgttgttga cccgagcaga atcacctaca
      661 gggactacat caacgcctgc agcgtgcttc tcgacgagaa caaacggaaa gtggtcgtgc
      721 agtttgtttt caacgaaaga gggaaacaga tccgattcct cacggaagac agaacggatt
      781 acgacaagta ccccatagaa gacttgaacg acatatacaa acacgccgac aggataatag
      841 cggaagctcg aaaatacgaa gccaaatagc ctctgcggct atggaagaga ttgagaactg
      901 aaacacggga ctcacgattt ctcgcaagtc ccgtgttcaa cttggtggag ctgacgggga
      961 tcgaacccgt gacctcatgt ctgccagaca ggcgctctcc cagctgagct acagcccctg
     1021 gttctcaaag aatgccgcta aaaagatagg cacgaaaccc gtgtttggca agtgaaaacc
     1081 atccgggtag ctacaccgcg cccatcgtct tcaggttctc aaagctgatt tttgcgctct
     1141 ccagcggcgg gcgcttgcac gaatcctgct ccacgatcaa ccacttcgat ccggcttcct
     1201 tcgctacggc gaaaatcgcg tcccagtcga ggatcccgtt gccgatttcc gcgaacgact
     1261 tcatgtcgtc gtccgccatg tccttgatat gcaccagcga aaggcgattg gcgaccttct
     1321 tcatgtacgt cgccgggtcc actcccgcgt actgcaccca gtaggtgtcg atttcggcat
     1381 ggagcgcctt cggatcgctg ttttcgtaaa ggatgtccag cccgtgcttg gcgccgaaga
     1441 gtttgaattc gtggttgtgg ttgtggtagg aaagttgaat gccttccttc agcagcttcc
     1501 cgccaatttc tgacagcaga gcagcagttc gtttccacgc ctcggcg
//
LOCUS       DAOQUC010001129         1545 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_110947_length_1545_cov_3.551007, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001129 DAOQUC010000000
VERSION     DAOQUC010001129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1545
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110947_length_1545_cov_3.551007"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(167..862)
                     /locus_tag="PKH07_16195"
     CDS             complement(167..862)
                     /locus_tag="PKH07_16195"
                     /inference="COORDINATES: protein motif:HMM:NF016051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HNT36528.1"
                     /translation="MARSILRGKNVLVTAGSTRAPIDAVRFISNRSSGRLATEIAYEL
                     LRRGATVTFLHGIGSETPQEHATPIQKRHLNTVAVESFDELHHSVPTLMNATRFDCVI
                     HAMAVLDYLPTEKIPGKIKSGRRELILKLAPAPKILPLFRMYAPNAFLVGFKLEVDVS
                     TEELCQRAHKLIEQAGADMVVANLMPSEKPDLHTAYLVHPNVAHSVNVPEEIVGKKQI
                     AWTVVEEVTKAIT"
     gene            complement(883..>1545)
                     /locus_tag="PKH07_16200"
     CDS             complement(883..>1545)
                     /locus_tag="PKH07_16200"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNT36529.1"
                     /translation="PDGTMTLEYLVSQYDGAIRYFDDQFAKILESLKSRGIYDNTVIV
                     FVSDHGEEFMEHGALDHGWTLYQEQLSVPFIMKPAGNEKIQWNLHGRVPEIIDIYPTV
                     VAAAGLVCAESNLAGRNLLVEGEPEHAFAEADKKNSMASLTGEGFKYIRQTKAKKAQL
                     FNLEQDPSEQNDLRNGSLDAKWDESVFQRVSESKERSLKPEIATVREAELERLRSMGY
                     VN"
BASE COUNT          349 a          408 c          383 g          405 t
ORIGIN      
        1 tcccgcactg cgaaggagcc gcacaattga ggcggctttg aacgctgcga tactgcctgt
       61 gacaccatag aggatatggc tgtttgtgat ttcagtagac atcatctctt cctttaggcg
      121 aactcctcct gtaagtgcaa gaatcagaca ccattcaaag gtgcggtcag gttattgctt
      181 ttgtgacctc ttccacaaca gtccaggcga tctgtttctt gccaacgatt tcctctggga
      241 cgtttacgct atgggcaacg ttgggatgaa cgagatacgc tgtatgcaga tcgggtttct
      301 ccgacggcat cagatttgca acaaccatat ccgctcctgc ttgttcaatc agtttatgcg
      361 cacgctggca gagttcttcc gtggagacgt ctacttccaa cttgaaacca accagaaaag
      421 cattgggagc gtacattcgg aacagaggca gaattttcgg ggcgggtgcc agcttcagaa
      481 tcagttcgcg ccttcctgat ttgatctttc ctggaatctt ctcagtggga agatagtcca
      541 acacggccat cgcgtggatg acacaatcaa aacgagtggc gttcataaga gttggaaccg
      601 aatgatgaag ctcgtcaaac gattcgactg caacagtgtt gagatggcgt ttctgtatcg
      661 gggtggcatg ctcttgcggg gtctcacttc cgattccgtg cagaaaagtg accgtagcgc
      721 cgcgcctcag cagttcatag gcaatctctg ttgccagtct tcctgatgat cgattggaga
      781 tgaaccgcac cgcatcaatg ggtgcccttg ttgagcccgc tgtcaccagg acattcttgc
      841 cgcgcaggat cgatcgtgcc atgaaagtga cctcctgacc cgctagttca catagcccat
      901 gcttcggagt cgttccaatt ccgcttcacg aacagtggcg atctccggtt tgagcgatct
      961 ttccttcgat tctgagacac gctgaaaaac actctcgtcc catttggcgt ccagagatcc
     1021 gtttctcaga tcgttttgtt cggacgggtc ttgttcgaga ttgaacagtt gcgccttctt
     1081 cgccttggtt tggcggatgt acttgaaacc ctctccggtc aacgaagcca ttgagttttt
     1141 cttgtccgct tcggcaaaag catgttccgg ctcgccctca accagcaggt ttctgcctgc
     1201 gagattggac tctgcgcaca caagtcccgc cgcggcaaca acggttggat agatatcaat
     1261 gatctctgga actcgaccat gcagattcca ttgaatcttc tcgttgccgg cgggtttcat
     1321 gataaacgga acactcaact gctcctggta caaagtccat ccatggtcga gtgccccatg
     1381 ctccatgaac tcctcaccgt gatcagagac aaacacaata acggtgttgt catagatccc
     1441 tctgctcttg agagattcga gtatcttggc gaactgatca tcgaaatatc gaatggctcc
     1501 gtcgtactgt gagacaagat actccaaggt cattgtgccg tcagg
//
LOCUS       DAOQUC010001130         1545 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_110975_length_1545_cov_2.688591, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001130 DAOQUC010000000
VERSION     DAOQUC010001130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1545
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110975_length_1545_cov_2.688591"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..397)
                     /locus_tag="PKH07_16205"
     CDS             complement(<1..397)
                     /locus_tag="PKH07_16205"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNT36530.1"
                     /translation="MYRILDETRKRLGERMLRFAQDLVRTPSESLREADLAERIEKEM
                     KYLGYDEVFRDGAGNVIGLMRGRRGDGTVLLNSHMDTVCAEPSSSWEKDPLCGEIEGQ
                     ILHGLGAADCKCGLAAQIYTGAILKDSLLP"
     gene            589..>1545
                     /locus_tag="PKH07_16210"
     CDS             589..>1545
                     /locus_tag="PKH07_16210"
                     /inference="COORDINATES: protein motif:HMM:NF018239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNT36531.1"
                     /translation="MRAIDLQVTELLSFSEGAIDFQGRRLVLHSLNAFAHLRRDLVEM
                     LGWQQTRRILTRFGYFWGHADAAAMKRVFQWDSLEEWIRAGERLHMLQGVAHSQIKSL
                     TVPPEKPFEMEIVWRESGEAREHLLEIGRTDAPICWMLVGYASGYCSFCLGRNVYFIE
                     DKCLAKGDRVCTATGKEADRWGSVLKSHLPYFEADDIRGKIKTLTKQIRERNREVDAQ
                     RKKINALERQHLPPTVEARSAEFQQVLDLARRVARFDTSVLITGESGVGKEVMARMIH
                     QCSNRASSVFLPANCAALPETLLESELFGHKSGAFTGANEDRR"
BASE COUNT          373 a          398 c          393 g          381 t
ORIGIN      
        1 ggggcagaag actatccttg agaatggcac ccgtgtagat ctgggctgcc aatccgcatt
       61 tgcaatcggc ggcaccaagt ccatgaagaa tctgcccttc aatctctcca cacagagggt
      121 ccttttccca actcgatgaa ggctctgcgc agactgtatc catgtgggaa ttgagcaaga
      181 cggtgccatc accacgccgt cctcgcataa gtccgatcac gtttcccgca ccatcccgga
      241 atacttcatc atagccgaga tacttcatct ccttctcaat acgctccgcc agatcggctt
      301 ccctgaggct ctcactcgga gttcgaacca gatcctgcgc aaaacgcagc attctttccc
      361 ccagcctctt tcttgtttcg tccaaaattc gatacatttg atttctcctt ttcgcagtat
      421 ggcgtccaaa gaacgctgac tgaaactaga gaaacttccg tgccaacaat gagatagaac
      481 atcatcacat tgataatcat agacttaaat tccttgacct tatgatttcg ctatgcaata
      541 ctatttcata atgtaatagc ctatatttca tactgaaagg ttctgttcgt gcgagctatc
      601 gatctgcagg ttaccgagct tctttccttc tcagagggtg ccattgattt ccagggcagg
      661 aggcttgtgc tccattctct caatgccttt gcacatttgc gtcgtgactt ggtggaaatg
      721 cttgggtggc agcagacccg aagaatccta acacggtttg ggtatttctg gggtcacgcc
      781 gatgctgccg cgatgaagcg ggtctttcaa tgggacagtc ttgaggagtg gattcgagcg
      841 ggggagcgac ttcatatgct ccaaggggtg gcacattctc agatcaagtc attgacggta
      901 ccacccgaga aaccttttga gatggagatt gtctggcggg aatcggggga ggccagagag
      961 catcttctgg aaatcggccg tacagacgcc cccatttgtt ggatgcttgt cggctatgca
     1021 agcgggtact gctctttctg tctgggaagg aacgtctatt tcattgaaga caagtgcctc
     1081 gcaaagggtg acagggtttg caccgccaca ggcaaggaag ctgacagatg gggaagcgtc
     1141 ctgaagagcc atctccctta ctttgaagcc gatgatatcc gtggcaaaat caagacactg
     1201 accaagcaga tacgggagag aaaccgtgag gtagatgctc aacggaagaa gatcaatgcc
     1261 ctggagaggc aacatttgcc acccactgtc gaggcacgaa gcgcggaatt ccagcaggtc
     1321 ttggacttgg ctcgtcgtgt cgctcgattt gacacatctg tcctgattac tggtgaaagt
     1381 ggtgttggca aggaggtgat ggcacgcatg atccatcagt gctccaacag agcctcttct
     1441 gtattccttc cagcgaactg cgccgccttg cctgaaacac tcttggagag cgagctcttc
     1501 ggtcacaagt caggcgcatt caccggtgcc aatgaagaca gaaga
//
LOCUS       DAOQUC010001131         1544 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_111118_length_1544_cov_2.391538, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001131 DAOQUC010000000
VERSION     DAOQUC010001131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1544
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111118_length_1544_cov_2.391538"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..714)
                     /locus_tag="PKH07_16215"
     CDS             complement(<1..714)
                     /locus_tag="PKH07_16215"
                     /inference="COORDINATES: protein motif:HMM:NF012879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNT36532.1"
                     /translation="MNQPSDHRFQSPLIPWNCEVLPNGMSLITKEVHSAPIVAIDIWA
                     ATGSGDETILNNGISHFYEHLFFKGTEKYPVGALDKAIKSFGGYDNAETSFDFTHYYV
                     VLPSEHCFTALEILTDAYLNASLPFEEIDRERMVVYEEIRRKEDSPTGKLFSDFLSHL
                     FPGIPYGMEVLGTEESLSRITRDDLLCFKEDRYCPSKTSLVIVGDFQADAIRRKVHDL
                     FEGFTNGSSQCDADFSAKPS"
     gene            complement(796..1140)
                     /gene="secG"
                     /locus_tag="PKH07_16220"
     CDS             complement(796..1140)
                     /gene="secG"
                     /locus_tag="PKH07_16220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HNT36533.1"
                     /translation="MVMFLLAVYFIICVLLIVAILIQSNKGTSIAGVFGGGSDTFLGA
                     SSAVTWLNKATTYLAVAFLVASFLLMLIPTMRGASTIPLDIPAGSEQSGAAPSAPIPS
                     DGGTTQPSSDLP"
     gene            complement(1254..>1544)
                     /gene="pgk"
                     /locus_tag="PKH07_16225"
     CDS             complement(1254..>1544)
                     /gene="pgk"
                     /locus_tag="PKH07_16225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HNT36534.1"
                     /translation="YAAEIQKARTVIWNGPMGYFENSEFAAGTKAVAKALADSNAMTI
                     IGGGDSASAVKKAGLADKMSHVSTGGGASLEFLEGIDLPGVAALDNKCCCCC"
BASE COUNT          396 a          405 c          399 g          344 t
ORIGIN      
        1 cgaaggcttg gcggagaaat cggcatcgca ctgagaggag ccgttcgtga aaccttcgaa
       61 cagatcgtgt acttttctac ggattgcgtc tgcttggaag tcgccgacaa tgacgagaga
      121 agtctttgat ggacagtagc ggtcctcctt aaagcacagt aagtcgtccc gcgtgattct
      181 cgaaagactc tcctccgtgc cgagcacttc catgccgtac ggtatgcctg gaaacagatg
      241 actcaaaaaa tcggagaaaa gtttgccggt gggggagtct tccttgcggc ggatttcctc
      301 ataaacgacc attcgttcgc gatcaatttc ctcgaaaggc agggaagcgt tgagataggc
      361 atccgtcagg atttccagtg cggtaaagca gtgctcgctg ggcaacacaa cgtagtagtg
      421 tgtgaaatcg aaggaagttt ccgcgttgtc gtaccctccg aaagatttga ttgctttgtc
      481 cagagcgccg acagggtact tttcagttcc tttgaagaac aagtgctcgt agaaatgaga
      541 gattccgttg tttagaatgg tctcgtcacc cgaaccagtt gccgcccaga tatcaatggc
      601 gacgatggga gcactgtgga cttccttggt aatgaggctc attccattgg gaagcacctc
      661 gcaattccac ggaatcagag gagactggaa acggtggtca gatggttggt tcattttcct
      721 taagataaag caaccggctg ccctgaaaag acaaagcagc cggtgactcc atttgttcta
      781 aagtaaactc agttgtcaag gcaagtcgct ggatggttgc gttgtgccac catcactcgg
      841 aatgggcgca gacggtgctg ctcctgattg ctcgctgccc gctggaatat ccaagggaat
      901 cgtactggca cccctcatcg tgggaatcag catcagcagg aacgacgcga ccagaaaagc
      961 gaccgcaaga taggtcgtcg ccttgttgag ccacgtgacc gcactggaag cacccaggaa
     1021 cgtatcactg cctcctccaa aaactccggc gatacttgtt cccttgttgg actgaatgag
     1081 aatcgccaca atcagaagca cgcagatgat gaagtagaca gccaacagaa acattaccat
     1141 tttgatacct ctcagcactc ttaagaaacc ggactacagg aagcccaatt tctatctgaa
     1201 acaagcaatc aagagagtgg acagagccca cccaccagct ggcgcacacc agctcaacag
     1261 caacaacagc acttgttgtc cagagcggca acacccggaa ggtcaatgcc ttcaagaaac
     1321 tccagagacg ctccaccacc tgtggaaacg tggctcatct tgtctgccag gcctgccttc
     1381 ttgactgcgg atgcggaatc tccgcctccg atgatcgtca tggcatttga atcggcgaga
     1441 gcctttgcga ctgccttggt gccagccgca aattcggagt tttcaaagta ccccatgggg
     1501 ccgttccaaa tcactgtcct ggctttctga atctcggcag cata
//
LOCUS       DAOQUC010001132         1544 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_111141_length_1544_cov_2.062458, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001132 DAOQUC010000000
VERSION     DAOQUC010001132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1544
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111141_length_1544_cov_2.062458"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..561
                     /locus_tag="PKH07_16230"
     CDS             <1..561
                     /locus_tag="PKH07_16230"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HNT36535.1"
                     /translation="EVQSGEQVLIMGPGPIGLMAARLCQLRGVEDIVIVGRSHSKARL
                     KAARQMGATQVIRSDLGEMSSLRTRHFHKALITASPSVIPEIMSFMRFGAIMAFLGID
                     FGPGGDVKIPMNDFHFSRLQLRASHASPALFFPEVLHLLGSGLISAQPLVSHVFTLNQ
                     FAQALKTAAEDKTSAIKVMIQNPELA"
     gene            complement(585..>1544)
                     /locus_tag="PKH07_16235"
     CDS             complement(585..>1544)
                     /locus_tag="PKH07_16235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HNT36536.1"
                     /translation="HRLSQGNVAVARVLYDAGFSVLTISNTMHPEFMRKAASSNLPGF
                     SEPDVEDVRHALRAIRQSIKERYSHRIVREALVGVSLGGFLTTLVAVNDVESPEDERF
                     DRYVAINTPVSLYRGMLGLDEYFTSPNQWAAEERHDRMEKALMKMAQLDVDQLESEDG
                     LPFDPLEARFLIGFGFRLKLRDVIYESERQRKSGRFRSNPSWIRRDDSYHEMMRYSYN
                     DYFERLLIPYSQERNHAWTRDHVLQSSDLRTQRSAIAKLSNLRVINNRNDFLVSSEDN
                     EWLSRVLGPDRLVLFDFGGHMGNLHLPRVQSFIVDAVRDLLQE"
BASE COUNT          377 a          408 c          386 g          373 t
ORIGIN      
        1 gaggtgcaat ccggagaaca agtcttgatc atggggccgg gaccaatagg tttgatggct
       61 gctcggctgt gccaactgcg tggagtggaa gatattgtca tcgtcggacg ctcccactcc
      121 aaggcacgct tgaaggcagc gagacaaatg ggggcaaccc aagtcatacg atcagacctt
      181 ggtgagatga gttcattgag aaccagacat tttcacaaag cgcttatcac tgcttcgccg
      241 tctgttatcc ctgagatcat gtcattcatg agattcggtg ccataatggc ttttcttggc
      301 attgactttg gacctggagg cgacgtgaag attccaatga atgattttca tttctcacgg
      361 cttcagctgc gggcttccca tgcttcaccg gcactgttct ttccggaagt gcttcatctt
      421 ctagggagtg ggcttatctc agcacagcct ttggtctcac acgtcttcac tctcaaccag
      481 tttgctcaag ccctgaagac agccgccgaa gacaagacct ccgccatcaa agtcatgatt
      541 cagaatcccg aactggcata ggacaggcac tacgagtgtg ttcatcactc ctgcagcaag
      601 tcccgaactg catcaacgat gaaggactgg actctgggca gatgaagatt gcccatgtga
      661 cctccaaaat caaacaacac aagacgatcc ggcccaagaa cacgactcag ccattcgtta
      721 tcttcagatg aaaccaagaa gtcatttcgg ttgtttatga cgcgaagatt gctcagcttg
      781 gcgattgcac tccgttgggt tcgaagatct gaggactgaa gaacatgatc ccttgtccat
      841 gcgtgattcc tctcctgaga gtagggaatc agaagcctct caaaatagtc gttataggaa
      901 taccgcatca tctcatgata ggaatcgtca cggcggatcc atgacggatt cgagcggaag
      961 cgacctgact tcctttgacg ttcgctctca tagataacgt ctctcagctt cagcctgaaa
     1021 ccaaagccga taaggaagcg tgcttccagc ggatcaaaag gcagcccgtc ttctgattcc
     1081 aactgatcca catccaactg tgccatcttc atcagcgcct tttccattcg gtcatggcgt
     1141 tcctctgccg cccattggtt gggtgaagtg aagtattcgt ccagtccgag catgccacga
     1201 tagagactga ccggcgtgtt aatcgccacg tatcggtcaa agcgttcatc ttctggagat
     1261 tcgacgtcat tcaccgcaac aagtgtcgtc agaaagccgc ctagagagac accaacaagg
     1321 gcttcccgca cgatgcggtg cgagtaacgc tctttgatgc tctgacgaat cgcccttagt
     1381 gcatgcctga catcttcgac atcgggttcg gagaatccgg gcagattgct actggcagcc
     1441 tttcgcatga attcggggtg cattgtgttg ctgatggtca agactgaaaa gccagcatca
     1501 tagagaactc gtgcgacggc aacgttgccc tgactgagcc ggtg
//
LOCUS       DAOQUC010001133         1538 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_111798_length_1538_cov_2.742414, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001133 DAOQUC010000000
VERSION     DAOQUC010001133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111798_length_1538_cov_2.742414"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1538
                     /locus_tag="PKH07_16240"
     CDS             <1..>1538
                     /locus_tag="PKH07_16240"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HNT36537.1"
                     /translation="FATKGVHIEDVFVSPKLLEEKARVEIHLSRNLETSATLKCRVTS
                     PSEHEVAYHEVNLDKCETDSERWHVGEPLVFSVPIKSPQPWSPESPALYKLRVTVSDN
                     KERTLAEREVRFGMRDVSIEGSRLLLNGKPLYVRGVLHWGYYPDLFTMDPSEEQIRRE
                     FEDFRAAGFNVVKVCLFLFPKRFYEIADETGMLIWQEYPIWQTFPQQAQPVDFERFSR
                     EFAEWFRFDRNHPSVILRDLACEAHQPDPDLMKAIYEMGKRMTDGAPLEDNSAYLNQV
                     YTDWYDCHMYRELDQLYDYLPSLAEHLRNQNPIRPYMSGEDLDEDTYRDCQSIRAAFA
                     NGSQMPWWLTNNNFRQQESFEKGLSENALGDVPKELVRRQNLHAIAFRKACFEEFRRF
                     PELNGYVMTSVRDTPPTRPGFYDDLMQTKWAGSTWLPFNSNRVLILHSLRRSRCFRMD
                     ETIRIDLVLSNFGDRVVDEPYHWQLLVGSSVVQEGTGNASAEPGTVTAIDSPTLDLPE
                     VLANT"
BASE COUNT          376 a          410 c          384 g          368 t
ORIGIN      
        1 tttgccacca aaggagttca cattgaggat gttttcgtct ccccaaaact tctggaagaa
       61 aaggcacgcg tcgaaatcca tttatcgcga aatctggaga cctctgccac cctcaagtgc
      121 cgagttactt ctccttcaga gcatgaagtg gcgtaccatg aagtgaatct cgacaaatgc
      181 gaaacggact cagaaagatg gcacgtcgga gagcctctgg tcttttcagt cccgatcaag
      241 agtccccagc cgtggtctcc ggagagcccg gcactctaca aactcagggt cacagtctcg
      301 gataacaaag aacgcacttt ggcagaacga gaagtccgtt tcgggatgag agacgtatca
      361 atcgaaggct caaggcttct tctgaatggc aagcctctct acgtccgcgg tgttttgcat
      421 tggggctact atcccgatct cttcaccatg gatccttccg aagaacagat tcgaagagag
      481 tttgaagatt tccgtgccgc gggcttcaat gtagtgaaag tgtgtctgtt tctgtttcca
      541 aaacgcttct acgagatcgc cgacgagaca ggaatgctga tctggcagga atatccgatc
      601 tggcagacgt ttccccaaca agcccaaccg gttgactttg agcggttttc cagagagttc
      661 gccgaatggt ttcgtttcga ccgcaatcat ccatcagtga ttcttcgcga tttggcctgt
      721 gaagcacatc agccagatcc cgatctgatg aaggccatct acgaaatggg caagcggatg
      781 acggatggag cgcctcttga agacaacagc gcctatctga atcaggtcta taccgactgg
      841 tatgattgcc acatgtaccg agaactggat cagttgtacg actacctgcc ttccctggcg
      901 gaacacttgc gaaatcagaa tccgataaga ccgtatatgt ccggtgagga tctcgacgag
      961 gacacttacc gagactgtca gagcatccgt gctgcgtttg caaacggatc tcagatgccc
     1021 tggtggctca ctaacaataa cttcaggcag caggaatcgt tcgagaaagg tctctctgaa
     1081 aatgccttgg gcgatgttcc taaggaactt gtccgtcgcc agaatctgca cgccattgct
     1141 ttccgcaaag cttgtttcga ggagttcaga cgtttcccag aactcaatgg atatgtcatg
     1201 acgagtgttc gcgacacacc gccgacaagg ccgggcttct acgacgatct catgcaaacc
     1261 aagtgggctg gtagtacctg gctgcctttc aacagcaacc gtgttctgat cctgcattca
     1321 cttcgacgct cccgatgttt tcgcatggat gagaccatcc ggattgatct cgttctctcc
     1381 aatttcggag atagagtcgt tgacgaacct tatcactggc aactgcttgt cggttcctct
     1441 gttgttcagg aaggtacagg caatgcctcc gccgaaccgg gaacggtcac cgcgatagac
     1501 tcacccactc ttgacctgcc ggaagtcctg gcaaacac
//
LOCUS       DAOQUC010001134         1537 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_111906_length_1537_cov_3.166667, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001134 DAOQUC010000000
VERSION     DAOQUC010001134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111906_length_1537_cov_3.166667"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..374
                     /locus_tag="PKH07_16245"
     CDS             <1..374
                     /locus_tag="PKH07_16245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36538.1"
                     /translation="DRVMTKARYAQVWVQRFASEDMKLSLAESLPESVQSLNDQQRKL
                     LAHLHDYLLKDDRTDMEIHNEIYEEAERLGLRSKEAFGAVYRVFLGASRGPRVGWFLR
                     ALDPDFVRKRLEEALAATGTC"
     gene            587..1357
                     /gene="pssA"
                     /locus_tag="PKH07_16250"
     CDS             587..1357
                     /gene="pssA"
                     /locus_tag="PKH07_16250"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HNT36539.1"
                     /translation="MRKVEIFPNLLTMGNLCCGLLAIVHLFDATPESMTWAARLILAA
                     LVFDALDGHVARLTKSASPFGVNFDSLADLTTFGVAPALLAYRMVFPLGDQIGVGLVV
                     IYAGCTALRLARFNSTATTKGKKPDFVGLPCPAAAAAVASLSMCIMKFELGPISALEM
                     GRPIAVLFMLSLSGLMVSKIPYPTAHRLGMAGAKPFNYLVIALLLLGLAAKHPSIAVF
                     LGSMTYVGLGIKEELVRRHRRERRTETAEEPETAPTPR"
BASE COUNT          340 a          398 c          424 g          375 t
ORIGIN      
        1 gggacagggt gatgaccaaa gcacgctatg cccaggtttg ggttcagcgc tttgcctctg
       61 aggacatgaa actcagtctg gccgaatccc tgcccgaatc agtccagtct ctgaacgatc
      121 aacagagaaa actcctggct catctccacg attatctgct gaaggatgat cgaacggaca
      181 tggaaatcca caacgagata tacgaggaag ccgaacggct agggcttcgc tccaaggaag
      241 cctttggcgc ggtctatcga gtctttctcg gtgccagcag gggtccccga gtgggatggt
      301 ttctgagggc attagatcct gactttgtga gaaaacgcct ggaagaggcg ttggcagcga
      361 caggcacttg ttagtcggct gcacgtgagg tagtggtgta atcaccatat gaaagaaaac
      421 cggattttcg ttttcataat tccccctcga tcccccttta acaagagggg tatggggggc
      481 tcgtgcgaag acgaatctgc cgattctcca catctgcaga atagatcggt tctttttgca
      541 gcaggccact tctcgatctg cgcccctttc agaaaaggaa ggtaccatga gaaaagttga
      601 gatatttccc aaccttttga cgatggggaa tctttgttgt gggctgcttg caatcgttca
      661 tctatttgat gctacgccgg aaagcatgac ctgggcagca aggttgattc ttgccgcctt
      721 ggtttttgat gctctggacg gacatgttgc ccgactgaca aaatcggcat cccctttcgg
      781 agtcaacttt gactctcttg cggacttgac cacttttggg gtggcaccgg ccttgctggc
      841 ctatcggatg gttttcccgt tgggtgatca gatcggtgtg ggtttggtgg tcatctatgc
      901 cggatgcacg gccttgaggc tggcgcgttt caactcaacg gcgacaacga agggaaagaa
      961 gcctgatttt gttggactgc cctgtccggc tgctgctgct gccgttgcat cgctgagtat
     1021 gtgcatcatg aagttcgaac tgggacccat ctcggctctg gaaatgggac gtcctatagc
     1081 ggttcttttc atgctctctc tgtcgggact gatggtcagc aaaatcccct atccgaccgc
     1141 gcaccgactg ggaatggcag gggcgaaacc cttcaactat ctcgtcatcg cactgttgct
     1201 gctgggactg gcggccaaac acccatccat cgccgttttt ctgggcagca tgacttacgt
     1261 gggtttgggc atcaaggaag aactggttcg ccggcaccgg agagaacgac gcacggaaac
     1321 cgccgaagag ccagagacgg ctcctacacc aaggtaagag ttctacctct gcaaacgact
     1381 catggtcgtg atggggtagg attgcagcat tgaacatgaa actcaaagtc cttcttctca
     1441 atcccccagg cgaccaactg taccttcgcg attactactg cagtcacgcc tccaaagcgc
     1501 gctactactg gcatccgtat gatctgctgg tgcagag
//
LOCUS       DAOQUC010001135         1536 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_112049_length_1536_cov_2.792708, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001135 DAOQUC010000000
VERSION     DAOQUC010001135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112049_length_1536_cov_2.792708"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            46..>1536
                     /locus_tag="PKH07_16255"
     CDS             46..>1536
                     /locus_tag="PKH07_16255"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HNT36540.1"
                     /translation="MDPTWPVVDAQESHGTEMAGLALFGDQLPELLSGNQRVQLSHRL
                     ESVRMLHNQQPHSEETWGYVTQASLAKAEIQTAQRNRVACLAVTAEDYGRDHGRPSSW
                     SAAIDQHSSGQLDDFRRLYVVSAGNIRDIHSNQDYRYPDAPLASSIEDPAQSWNALTV
                     GAFTNQTQIRSAHFDGYQPVARRGGICPTSRTSYSWGDQAWPFKPDITMEGGNYVQSP
                     AGNIDGCEDLALLTTKLDATGRLLTCSGDTSAATAQAARYAAILMADYPHLWPETVRG
                     LLVHSAEWTQEMQRQLPGTRETDRHNRIRCFGYGVPNLDRARYTVENCVSLIHQGTIQ
                     PYKLDGTEAKTNHFTLHSLPWPKAALEQMHDHDVTVKVTLSYFIEPSPAGRGWGRKFR
                     YASHGLRFALRGPTETESQFRQRISKQEWEEEQEGRPSTSDPINWAVGVRLRTKGSIH
                     CDWWTPQGGAAELARCGQVAVFPVTGWWRERKHLGVVERQTRYSLII"
BASE COUNT          366 a          422 c          410 g          338 t
ORIGIN      
        1 catcctcttt tgcggcccct aatcactgac gaagacaccc agtcattgga ccctacatgg
       61 cccgtcgtgg atgcacagga gtctcatggc actgaaatgg caggactggc tttgttcgga
      121 gatcaattgc ctgagttgtt atccggaaat caacgtgtcc aattgagcca tcgtctcgaa
      181 tccgtacgca tgttgcacaa ccagcaaccg cattccgagg aaacgtgggg atatgttacc
      241 caggccagcc tggcaaaggc tgagattcag acagcccaac gtaatcgtgt cgcctgtctt
      301 gccgtaacag cagaagacta tgggcgtgac catgggcgcc cttcatcctg gtctgcggcc
      361 attgatcagc attcttccgg ccaactggac gactttcgca ggctctatgt ggtatccgcc
      421 gggaacattc gggatattca ctctaaccaa gactaccgtt accccgatgc accgctggca
      481 agtagcattg aagatccggc ccaaagctgg aacgcgttga cggtgggtgc atttaccaat
      541 cagacacaga tccgttcggc ccattttgat ggttatcagc cagtggcacg ccgaggtggc
      601 atatgcccga ccagtcggac atcctactcc tggggagacc aagcctggcc cttcaagccg
      661 gatatcacca tggagggcgg caactatgtg caatcaccag ccggtaacat cgatggatgt
      721 gaggatctcg ccctactgac aacgaagctc gatgcgaccg gtcgtttgct gacatgttcc
      781 ggtgacacca gtgcagcaac ggcacaggca gcccgctatg cagccattct catggcggac
      841 tatccacacc tttggcctga aactgttcgt ggcctgttgg ttcattctgc ggaatggact
      901 caggaaatgc agagacagct cccgggaacc agagaaacag accgtcacaa cagaatccgt
      961 tgttttggct atggtgtccc caatctcgac agggcacggt ataccgtgga gaactgcgtc
     1021 tcccttatac accagggaac catccaacct tataagctcg atggcacaga ggctaagacc
     1081 aatcatttca cacttcactc tcttccatgg cccaaagctg ctcttgaaca aatgcatgac
     1141 catgacgtca ccgttaaggt gacgctttct tacttcattg aaccgagtcc agcaggtcga
     1201 ggatggggca ggaagttccg ttatgcatcc catggtttgc gttttgcctt gcgaggtcct
     1261 acggaaacgg aatctcagtt caggcagcga atcagcaaac aggaatggga ggaggaacaa
     1321 gaaggacgcc cctcaaccag tgatcccatc aactgggccg ttggggtgcg tctacgtacc
     1381 aaaggttcga tccactgcga ttggtggacg ccccaaggag gtgcagccga attggcccgt
     1441 tgtggtcagg tcgcggtttt tccagtcact ggctggtggc gtgaacgaaa gcacttgggg
     1501 gtcgtagaga gacagactcg atattcattg attata
//
LOCUS       DAOQUC010001136         1535 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_112151_length_1535_cov_3.237162, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001136 DAOQUC010000000
VERSION     DAOQUC010001136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1535
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112151_length_1535_cov_3.237162"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..375)
                     /locus_tag="PKH07_16260"
     CDS             complement(<1..375)
                     /locus_tag="PKH07_16260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020122488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNT36541.1"
                     /translation="MTQTQIVIPECSLVFLIGTSGSGKTTFARKHFKQTEVLSSDFFR
                     GLIADDENDQTATQDAFEVLHLVAEKRMASGRTTVIDATNVRSDARKPLLDMALDYDF
                     TPIAIVLDLPLDVCLKWNSVRPD"
     gene            complement(479..>1535)
                     /locus_tag="PKH07_16265"
     CDS             complement(479..>1535)
                     /locus_tag="PKH07_16265"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNT36542.1"
                     /translation="EMRVRLQRDYSLLPGHRWFLPAHWLLGLNEVLAEEERITVLTGA
                     RCSEVLVDESGRVCGGVASCGGYTVTVKAKVSIDATGTGFFSEAAGCQTMYGRDARAT
                     FNESLAPEEADDQVQQVTQMYVSQRMPGASGFDMTQLEHVRLGVLVSEVGWFHQHKEK
                     AMEKDLGIHLHWGCAVKCRDTRDPVAIADAQREALVKLKGDLELLRQNRYAVHLAPKL
                     GVREVRRVVGEYVIAQDHLVAGAYPEDTIAICGYGLDIWGENTGAVHSKKYGIPLRAL
                     IPKGVSGLLVTGKCASGSHIAMSSYRVIPIVGSMGQAAGIAAALCSKLNVEPRNLEAE
                     KVRAHLIQPQHKVTLEL"
BASE COUNT          352 a          471 c          372 g          340 t
ORIGIN      
        1 gtctggccgc acactgttcc atttcagaca aacatccagt ggcagatcaa gaacgatcgc
       61 aatgggagtg aaatcgtaat caagtgccat atcaaggaga ggctttcgag catctgagcg
      121 cacattggtc gcgtctatga ccgttgttcg acctgaggcc atcctcttct ctgccaccag
      181 atgaaggact tcaaaagcgt cctgtgttgc agtctggtcg ttctcatcgt cggcgatcag
      241 cccccgaaag aaatccgaag agagaacctc ggtctgcttg aaatgcttcc gtgcaaaggt
      301 agtcttaccg gaaccggatg ttccaatcag aaagacgagt gaacactcag ggataacaat
      361 ctgtgtctga gtcatcgaat cggttcacac tcccttggtg aggtgccaag caaaccagca
      421 cgactaaact tttcgccagg aagaatcagc gccaacaaca agcactgcct gctccgaact
      481 acaattcgag cgttactttg tgctgtggct gtatcagatg cgcccgaacc ttctcggctt
      541 cgaggttcct cggttcgaca ttcagtttgg agcacagtgc cgccgcaatt cccgctgcct
      601 gtcccatgct ccctacaatt ggaatcacgc gataggagct catggcgatg tgactcccgc
      661 tggcgcactt gcccgtcacc aacaaaccgg aaacgccctt agggatcaac gcacgtagag
      721 gaatcccata cttttttgag tggactgcgc cggtattctc accccagata tccaaaccat
      781 agccgcagat ggcgatggtg tcctcaggat aagcccccgc aacaagatga tcctgagcaa
      841 tgacgtactc tccgacaaca cggcgcactt ctcgaacgcc aagtttcggt gccagatgga
      901 cagcatagcg attctgacga agcagttcga gatctccctt gagtttgaca agtgcttccc
      961 tctgggcatc agctatggca accggatctc gggtatcccg acacttcacc gcacatcccc
     1021 aatgcagatg aatgcccaga tccttctcca tggccttttc cttgtgctga tgaaaccaac
     1081 cgacttccga gaccaggact ccgagccgga catgttccag ctgagtcatg tcaaagccac
     1141 ttgcgcccgg catcctctga ctgacgtaca tctgggtgac ctgctgcacc tgatcgtctg
     1201 cctcttcggg tgcaaggctt tcattgaacg tcgcccgggc gtcgcgccca tacattgtct
     1261 ggcatcccgc tgcttccgag aagaaacccg ttcccgtcgc atcaatgctc accttggctt
     1321 ttacggtcac cgtgtatcca ccgcacgaag ccactccgcc acagacgcgc cctgattcgt
     1381 ccaccagaac ttccgagcag cgtgcccccg tcagaacggt gattcgttcc tcttcagcca
     1441 ggacttcgtt cagcccaagc agccagtgtg caggcaggaa ccatcggtgt ccagggagaa
     1501 ggctgtagtc ccgttgcaat cggactcgca tctcc
//
LOCUS       DAOQUC010001137         1535 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_112190_length_1535_cov_2.486486, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001137 DAOQUC010000000
VERSION     DAOQUC010001137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1535
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112190_length_1535_cov_2.486486"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..649)
                     /locus_tag="PKH07_16270"
     CDS             complement(<1..649)
                     /locus_tag="PKH07_16270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36543.1"
                     /translation="MQKSTLRFLIVFCLILSVVPVGFAQQSLQDVIEKGERQIRSKPR
                     ASSAVMEVGEPGSAREIRYEVAPAAMAAAAKSALVAIGQKTANGTFAATGMGFYAGPG
                     GLILTLDICIGADDPILVMTTEGQVLEADYVASDDRVGVAVLDTDEDSFYLPLGGSES
                     LSLMSRLMVMQPGGSDPQQWGTSSPAVQAVPCNILPPRPDQPQVDRPDFVEVDLAL"
     gene            complement(781..>1535)
                     /locus_tag="PKH07_16275"
     CDS             complement(781..>1535)
                     /locus_tag="PKH07_16275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36544.1"
                     /translation="ESSSGLSHVFHARSDWFAEMVDPNIEPGHGISLDISTSGTIHAV
                     FYDSSIRTLRYAWKERGEAWRFESIVSDASAAIFVPRVQVDTLGTVHVVYCHQAPAAD
                     GATVRYVFRRDGVWMPAVDLSAAGGITRLAKGQSNVPLTLDLDPSDRPWVAFEPLSGD
                     ISLAVWNSAFWESISFSRPGNHILSDYHVSMVVDRRGGIHLACSFSDNGGETKVYYFR
                     GILKKVPHCTDYNQDGFVDEQDLMMLLPGWGQ"
BASE COUNT          416 a          447 c          359 g          313 t
ORIGIN      
        1 cgagagccag atccacctcg acaaaatctg gcctatccac ttgaggctga tcgggtctgg
       61 gggggagaat gttacagggc actgcctgta cggcaggcga tgatgtcccc cactgctggg
      121 gatcagaacc gcccggctgc atcaccatca acctcgacat caaactgagg ctctcggaac
      181 cacccaatgg aagatagaaa gagtcctcat ctgtatccag gactgcaact ccgactcgat
      241 catcggaggc gacgtagtca gcttcaagga cttgtccttc ggtggtcatg acaagaatgg
      301 gatcgtccgc accaatacag atatccaagg tgagtatcag ccccccgggg ccagcgtaga
      361 atcccattcc tgtggccgcg aaggtaccgt tcgcagtctt ctgaccgatg gccaccaagg
      421 ctgatttggc agccgctgcc attgctgcgg gagccacctc atagcggatc tcacgagcac
      481 tccccggctc cccaacctcc atgactgccg aagaagcacg gggcttcgaa cggatctgcc
      541 tctctccctt ctcaataaca tcctgaaggc tctgctgggc aaagccgacc ggaacaacag
      601 acaggatgag acagaaaacg atcagaaacc gtagtgtgga tttttgcatt atcctatatc
      661 tcctttcagt gtcacaaaga actccatcca tattccttaa tgccgaagag tttacatttc
      721 agataatata ctacccgctc tgttgtggca acacgtttgc cgtacaccca agaaacagac
      781 ctattgcccc cagcccggaa gcagcatcat caagtcctgt tcgtcaacaa acccatcctg
      841 attgtagtcg gtgcagtggg gcaccttttt cagaatccca cgaaaatagt aaaccttcgt
      901 ctctccgccg ttgtcggaaa aggaacaggc cagatgaata ccacctctcc gatcaactac
      961 catggaaaca tgatagtccg acagaatgtg attcccaggg cgcgagaagg agatgctctc
     1021 ccagaaggca ctgttccaaa ctgcaagaga aatatctcca ctcaaaggct caaaggctac
     1081 ccagggacga tcactgggat ccaagtccaa tgtcagaggc acattgctct gtcctttcgc
     1141 aagacgcgta atccctccag cggcgctcaa gtcaacagca ggcatccaca ccccgtctct
     1201 gcgaaaaacg taccgaacag tggcaccatc agcagctgga gcctggtgac aatagacaac
     1261 atgaactgtt ccgagtgtat caacctgaac acgcggtacg aagatcgcag cactcgcatc
     1321 cgaaacaata gactcaaaac gccaggcttc cccgcgctct ttccaggcat acctcaatgt
     1381 tcgaattgaa gaatcataga agacagcatg tattgtgccg gatgtactga tgtccagaga
     1441 aatgccatgg cctggttcaa tgttcggatc gaccatctca gcaaaccaat cacttcgtgc
     1501 atgaaagacg tgtgacaagc cactggagga ctcat
//
LOCUS       DAOQUC010001138         1533 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_112399_length_1533_cov_3.203654, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001138 DAOQUC010000000
VERSION     DAOQUC010001138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1533
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112399_length_1533_cov_3.203654"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            199..708
                     /gene="flgN"
                     /locus_tag="PKH07_16280"
     CDS             199..708
                     /gene="flgN"
                     /locus_tag="PKH07_16280"
                     /inference="COORDINATES: protein motif:HMM:NF016985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FlgN"
                     /protein_id="HNT36545.1"
                     /translation="MEASSDRIPDQDNRESASSEALQVKAALLQGFGRQLVIYRELTT
                     LAQQLSVAISDGDHQRLLQLVAHKQERVESLALIDVSLACAKNSWAGLREQVSDGDRA
                     EIRNCVVQIERVLKELLSLEAANEQALSILAQQTQKDVQAAQTERRARSAYNLPPAGN
                     DPGVLDKRG"
     gene            836..>1533
                     /locus_tag="PKH07_16285"
     CDS             836..>1533
                     /locus_tag="PKH07_16285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNT36546.1"
                     /translation="MNGSDSSQGLGTVHAGDITALARLFQEFQASTDALQQAYDRLQK
                     RVAEIDAELEVKNAELEQANQALQRKMAELDDVRQYLANVLESMNSGVVTIGTDGRIT
                     MVNPTASCWFQGESSTLQGRPITDFFPIHFVPCVLNALSSGERFSDVEESLIALDGSE
                     RFIRTSAAPLFDATGNRIGALMIFNDITQLRLLQEKARRQDRLAALGELAAGVAHEMR
                     NPLTTIRGYIQILPT"
BASE COUNT          350 a          392 c          456 g          335 t
ORIGIN      
        1 tttcgagcag gcagcgcctg cgggcgcgtg ggctatgcct actcttgtgc catgcccgct
       61 tgcgaaagag actgcctcgt tcggagtctg gttttctggg agcaggccag cgcctgtcat
      121 aagagcgcta agtccgctct ccagtattcg tagaccgtgc tcttgtagag cgcaatcttc
      181 tctgtgcagg aggctctggt ggaggcttct tccgaccgaa tcccagacca agacaacagg
      241 gagagtgcat cctcggaagc ccttcaggtc aaggccgctt tgttgcaagg gtttggacgg
      301 cagctggtca tctatcggga attgacgacg ttggcgcaac agctcagcgt ggcaatctcc
      361 gacggggatc atcaacgact tctccaactg gtggcgcaca agcaggaacg cgttgagtcg
      421 ctggctctga tagatgtaag tctggcttgt gcaaagaata gttgggcggg gctgcgtgaa
      481 caggtttcgg acggggacag ggcagagatc aggaactgtg tggtgcagat cgaacgggtt
      541 ctcaaagaac tgctttctct ggaagcagcc aacgaacagg cgctctccat tctagcgcag
      601 cagacgcaga aggatgttca ggctgctcag actgaacgac gggcacgttc cgcctacaac
      661 ctgccgcccg caggaaacga tccgggggtc ctggacaaga gaggttgatg cgggaaatcc
      721 tgatgtcttt cggccagaca cagatcatgg agaaaatgca aactgaggat agtcggcaga
      781 gtagcgcaag gtgagcatga caaaccaagc aacaaaagag aattcttctg cttcgatgaa
      841 tggttccgac tcgtcacagg gtctgggtac agtccatgca ggggatatca ctgcgcttgc
      901 acggcttttt caggagttcc aggccagcac agatgctctc cagcaggcgt atgatcgcct
      961 tcagaagcga gtggcagaga ttgacgcgga gctggaagtg aaaaacgctg aactcgagca
     1021 ggccaaccag gctcttcagc ggaaaatggc cgagctggat gatgtgcgtc agtaccttgc
     1081 caatgtgttg gagagtatga acagcggcgt agtgaccatc ggcaccgacg gccgaatcac
     1141 catggtgaat cccacggctt cttgctggtt tcaaggagag tcctcaacgc ttcaaggtcg
     1201 cccgatcacc gactttttcc ccattcattt tgtgccgtgt gtacttaacg ccttgtcttc
     1261 gggtgagcgt ttctctgatg tggaagaatc gcttatcgcc ttagatggca gcgaacggtt
     1321 cattcgaacc agtgccgctc ccctttttga tgcgacaggc aacagaatcg gtgccttgat
     1381 gatattcaac gacattaccc agttaagact gcttcaggag aaagcgcgtc gccaggacag
     1441 gcttgcggct ctgggagaat tggccgcggg tgtggctcat gagatgcgca atccgctgac
     1501 gacgattcgc ggatacattc agattcttcc cac
//
LOCUS       DAOQUC010001139         1533 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_112414_length_1533_cov_2.910690, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001139 DAOQUC010000000
VERSION     DAOQUC010001139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1533
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112414_length_1533_cov_2.910690"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..239
                     /locus_tag="PKH07_16290"
     CDS             <1..239
                     /locus_tag="PKH07_16290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36547.1"
                     /translation="NEFVTALGQMYLHEPQTIMAEVDRKGKQESGYGQHFPENRRRKT
                     AGPGAPVKQRSDRSGAGKNRPRSALRKGAGVGPR"
     gene            223..549
                     /locus_tag="PKH07_16295"
     CDS             223..549
                     /locus_tag="PKH07_16295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36548.1"
                     /translation="MWDRGERVPVVSTRSPVEGRDWGPSTRAWVGCEWQKPQRYVLEG
                     VNGARRVHGAESGGAGSAAAPCVAFPGELWAAVRDVGIMTDYVKAGRQNRGRGQRTLA
                     WNHRNW"
     gene            763..>1533
                     /locus_tag="PKH07_16300"
     CDS             763..>1533
                     /locus_tag="PKH07_16300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36549.1"
                     /translation="MKKPWVYKRKSIKGWWCGWYEGGKRKAKALPSKALAEHFRHMKY
                     AQLNSDVFTSVVDFEWWHMVEEYQQFKQVGGRKEASIYEVLLTLRHFERLVGANSSRE
                     ITQQNLDAFVLSRGEEVGKNTLNKDIANLRAFLNWAVRNRFVGSGLEIRKVKVAQKPV
                     VALSVQQVRDLLTATAKYPTLRLRVLLAVTTGLRRGDIEVIRIGDIHFDRNTIATRNR
                     KAGKAMPERPMPEAIMTELSNYVVTLQDGQERLFSDRFS"
BASE COUNT          364 a          376 c          500 g          293 t
ORIGIN      
        1 ccaacgagtt cgtcacagct ttgggccaga tgtacctcca cgagcctcag actattatgg
       61 cggaagtcga ccgcaagggc aagcaggaaa gtggctacgg acaacatttt cccgagaaca
      121 gacggcggaa gacggcggga ccaggtgcac cggtcaagca gcggtcggac cgctctggag
      181 cgggcaagaa tcggcctcga agcgccctca ggaaaggggc aggtgtggga ccgcggtgaa
      241 agggtgccag tcgtttcaac gcgatcgccg gtcgaaggca gagattgggg gccatcaact
      301 cgtgcatggg taggctgcga atggcagaag ccccagagat atgtgctcga aggtgtcaac
      361 ggggctcggc gagttcacgg tgccgagagt ggaggggcag gttccgcagc cgctccgtgc
      421 gtcgctttcc ctggggaact ctgggctgct gtcagggatg tgggaatcat gacagactat
      481 gtgaaggcgg ggaggcagaa caggggcaga gggcagagaa cgctggcttg gaatcaccgc
      541 aactggtgag aggtgcacaa tgcctactgg atgagacgga gtattcggac gggttgttct
      601 atacccccga aaggtccacg ttcataagtc tagccagaat agaggcttat aatgccatga
      661 tccgtgtctg actggcgcat gtctccattt tgtcactgtc gggatcgcca tagcttcccg
      721 gcaatcgcct cggaagcgaa aactgccgat atgatggaga ctatgaagaa gccatgggta
      781 tacaaacgca agagcatcaa aggatggtgg tgtggctggt acgaaggagg caagaggaag
      841 gccaaagcac tgccgagtaa agcattggcg gagcactttc gccatatgaa gtacgcgcaa
      901 ctcaattcgg atgtgttcac gagcgtcgtt gactttgagt ggtggcacat ggtcgaagag
      961 tatcaacaat tcaagcaggt cggaggtcgg aaggaggctt cgatctacga ggtcctgctg
     1021 acgctgcgac actttgaacg actggttggc gccaattctt ctcgggagat cacgcagcag
     1081 aatctggatg cgttcgttct cagccgcggt gaggaggtgg ggaagaacac gctcaacaag
     1141 gacatcgcaa atctacgggc cttcctcaac tgggctgtca ggaatcgttt cgtgggttcg
     1201 ggcctggaga tcaggaaggt caaagtggca cagaaaccgg tggttgccct gtctgtccag
     1261 caggtgcgag atctgctcac ggcaacagcg aaatacccga cgctccgcct gcgggtattg
     1321 ctggccgtga ccacaggtct tcgacggggc gacatcgagg tcatccgcat tggcgacatc
     1381 cactttgacc ggaataccat cgcgactcgg aatcgtaagg cggggaaggc tatgccggaa
     1441 cggccgatgc ctgaggcgat catgacggaa ttgtccaact acgttgtcac cctacaagat
     1501 ggccaagaaa ggctcttttc ggatcggttc agt
//
LOCUS       DAOQUC010001140         1531 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_112653_length_1531_cov_3.039973, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001140 DAOQUC010000000
VERSION     DAOQUC010001140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1531
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112653_length_1531_cov_3.039973"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            742..1302
                     /locus_tag="PKH07_16305"
     CDS             742..1302
                     /locus_tag="PKH07_16305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36550.1"
                     /translation="MTIEQFIFLSPLFGFLCVLLGQTRMHLYAHTRARPFLYFGLTAM
                     ASGLWFYFLAFLTADILPFPVDVVFTVAVLLGAPVLAIAALVVTSNINVSGECRKVKR
                     NLSYIRYVFGIPQRQGISTDATDSQEGIVSQYRMLGIGLICLCTATAAMLMLRISLLA
                     LIYVLFGYWMLLKQWGCVPRWPWRNI"
BASE COUNT          317 a          347 c          415 g          452 t
ORIGIN      
        1 ggagggtgca gaggtacggg acggggaata acaaagaaac tgtgtttggt tattcttcaa
       61 cttactctga cgttcccagc agtacggagc agtatgtgta cgctgtggac ggttccagga
      121 atactgtcgg tgtgtggggc tctgatgggc ttgctattgg gaaggcgtct cttgaacagg
      181 atgcctttgg ctgtcagata aatgattcgt ggaatgtgca acacacaagg caccttgcaa
      241 gcctggaaca tgatgcggac attggtgcag tgcttctcgg cacacgttgg tactctccag
      301 aaaggggcat gtactttgtg catggaagcc cctatggcga gtttctttca ggctctcaca
      361 acagctatgt gcagacacat ctattgtaca tgaaggatgc acagtctggt tgtgtttctg
      421 gatcttggtt ttgtctctca aggcgaccgc caattagtag aaggatatac gattttctcg
      481 agaaacatca ccatttgtgt gatatcggtg ttcatcttct gtgtcatttc gcatgtcata
      541 cgttaggtgg aaacttcctc tgtttccccg cttgtttcgc cgttgctgca atcttgtgtc
      601 ctcccccgga cgcacatggc tgtcctgctc cggttccact cgaaggtgtt ccgagtccag
      661 ttcttcctca tgctctccat agagaagccc cacttggagt tttgcctttg aatgtagcca
      721 gtgttcactg aggatagtaa gatgacgatt gaacaattca tatttctttc accactcttt
      781 ggattcttgt gtgtcttgtt gggtcagacg cggatgcacc tttacgcaca tacacgcgcg
      841 agaccgtttc tttactttgg gttgactgct atggcaagcg ggctgtggtt ctacttcttg
      901 gctttcttga ccgccgacat cctgcccttt ccggttgatg tggtctttac tgtggcagtc
      961 ctactcggag ctcctgtcct ggcaattgcg gcattggtag tcacttcaaa tataaacgtc
     1021 tcaggcgagt gtcgcaaggt caagcgcaat cttagttaca tcagatatgt gtttgggatt
     1081 ccccaaaggc agggaatcag tactgatgca acggattcac aagaagggat tgtatctcag
     1141 tatcgaatgc ttggaattgg tttgatttgt ctttgtacag ctactgctgc tatgttgatg
     1201 ctccgaatat ctttgctcgc tctaatctat gtgctctttg ggtattggat gcttctgaag
     1261 cagtggggtt gtgtgccaag gtggccttgg cgaaacattt aggattgggt ggaacatcta
     1321 ccagtacaga ggtgctggac gtgttcggaa ctgtgtgagc cagcagaatg ggtgaaaccc
     1381 atcaattagc ggtgattttc taactgatgg gtcatgatcc atcctacgag ctcttgggaa
     1441 cgccgagcgt ctgctcggca gcgagtagcc cggtttcccc ggtgcgccgt ggaaaggcgc
     1501 gcactttcag tgggaggtag acccacctgg c
//
LOCUS       DAOQUC010001141         1530 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_112836_length_1530_cov_2.118644, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001141 DAOQUC010000000
VERSION     DAOQUC010001141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1530
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112836_length_1530_cov_2.118644"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1517)
                     /locus_tag="PKH07_16310"
     CDS             complement(<1..1517)
                     /locus_tag="PKH07_16310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36551.1"
                     /translation="MLITILTFYHALLYPVTYWDSLILYTHYGEMTYREQGFPVRVCA
                     QVGLGLGANYPHYYPLTSATLATFWGGWDDLYAQMFPPLAGLISVVLLYDLLLTLFES
                     RRTAILVTLLFRSFAYATCYFIWATDYALVMMLATAILWLCQRILNQFRETSIAVSRR
                     EMSLEMYVVLTVALVAFAMHINYLGIVLVIPLLVTLLHIPNRPVPAGVSAMPTATNVC
                     SLEEPGFFRVLFPRRLRASRTLFGFGILIACGLCLLGGSWYLRNWIVTGNPVYAFFPR
                     LLGGRYVDLDVLASCAQEWTSNGDGVARFGSTLQERILRSPEYFLLQTNWHWKFGPTL
                     FGLCIPGIFLIHTATKRVRSLMAVAGSLLLAGLVFEYFISGLYLYHILFVTPCLSCLS
                     GVAVHRLLKRRWSRRYAICLVLLIGLTPGLSMALVGPKLMSFDLKAFRCPGIDKDEFW
                     SMCFQRDQEMWRTINDRLPLNAGVLTHENRYHVFRRDIRIVHGTTGNSQGSTGSP"
BASE COUNT          448 a          392 c          390 g          300 t
ORIGIN      
        1 aaagggcttc ccgtagagcc ttgagaattc ccagtcgtcc catggacgat tcggatatct
       61 cttcggaaga catgatatct gttttcgtgt gtcaacacac ctgcattcag tggaagacga
      121 tcatttatcg tacgccacat ctcctggtct cgctgaaagc acatgctcca gaactcatct
      181 ttgtcgatcc ctggacacct aaatgccttc aaatcaaaac tcatgagctt cggacccaca
      241 agtgccatag aaagccccgg agtcagacca atcagtagaa caagacagat cgcatatctc
      301 cgtgaccacc gtctcttaag aagtctgtga actgcaaccc ctgacaggca ggaaaggcaa
      361 ggcgtaacga agaggatgtg gtacaggtag aggcctgaga tgaagtactc aaacaccagc
      421 cctgcaagga gaaggcttcc tgctactgcc atcagactgc gtactctctt cgtcgccgta
      481 tgaatgagaa agattccagg aatgcataga ccaaaaagag tcggaccgaa tttccaatgc
      541 cagtttgtct gcaggagaaa gtactcaggc gacctgagta tcctctcctg tagggtcgag
      601 ccaaatcggg caactccatc accattgctc gtccattcct gagcgcagga agcaagtacg
      661 tccagatcaa catacctgcc accaagcagt ctcggaaaga aagcgtagac aggattacct
      721 gtcacaatcc agttgcgaag gtaccagctg ccaccaagaa gacaaagtcc gcaggcaatg
      781 agtatgccga acccgaaaag agttctcgat gctcggagtc tcctgggaaa caagactctg
      841 aagaagcctg gttcttccaa tgaacaaacg tttgtagcag tgggcatagc cgaaacgcct
      901 gcaggcactg gcctgttcgg aatatgaagc agagtcacga gcaatgggat gacgagcacg
      961 attcccaggt agttgatgtg catcgcaaat gccacaagag caacagtcaa tacgacgtac
     1021 atctcaagtg acatctccct cctggagacg gctatcgagg tttcgcgaaa ctgattcagg
     1081 atacgttggc aaagccacag gatggccgtg gccagcatca ttaccaaagc gtagtcagta
     1141 gcccagatga agtagcatgt agcatacgca aaactcctga agagcaacgt aacgaggatg
     1201 gcagttcgcc gtgattcgaa cagtgtgaga agaaggtcgt agagaagaac aacgctgatc
     1261 aaaccggcca gcggaggaaa catttgtgca tagagatcat cccagccgcc ccagaacgtt
     1321 gccagggtcg ccgaagtcag gggatagtag tgagggtagt tggctcccaa gccgagcccc
     1381 acctgtgcac acacgcgaac aggaaagccc tgttcccgat aggtcatctc gccgtagtgc
     1441 gtgtagagaa tcaaggaatc ccaatatgtc accggataga ggagggcatg atagaacgtc
     1501 aaaatggtga tgagcattat cagagcccat
//
LOCUS       DAOQUC010001142         1529 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_112890_length_1529_cov_3.675034, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001142 DAOQUC010000000
VERSION     DAOQUC010001142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1529
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112890_length_1529_cov_3.675034"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1323
                     /locus_tag="PKH07_16315"
     CDS             <1..1323
                     /locus_tag="PKH07_16315"
                     /inference="COORDINATES: protein motif:HMM:NF019368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HNT36552.1"
                     /translation="YLPPPTAQLRHWGHFIKRPVTPVDIGQGPYATVKVEDESGRAVP
                     AQIVDGKLFPNGYRLKVQFVAESVPGCGLRTYYVTPEESGSAGSDGIVVRDTTIETPL
                     LKVVVDAKTGHLLRIYDKRQRRDVLGKNTRANVLRLCMEAPHGMTGWVIGPVNSVEDL
                     DKAESVQVVEQGPVRAVIQVERKWDRSTFTQRIIVYKDLPRVDFELDVRWFQLGNHEV
                     GVPMLRVAFPLNVTNGRFSCDTPFATVERPTTGQEVPAQKWTDLSGPNGGAALLNNSK
                     YGHRCEGKTLEMTLLRSSYDPDLYPDQGPHHIRYSLFPHAGSWDEAAVDLEGTSFNFP
                     LLAIEATPSEQGDLASGTSFLTLNPGNLLLSAIKRAEDGASLVVRFYESQGRKSHALL
                     RFNRAVKCVTRVNLLEEPLDGVPDATLSGKKVMVPVKPHEIVSLRVDF"
BASE COUNT          351 a          377 c          435 g          366 t
ORIGIN      
        1 tatttgccac caccgacggc tcagcttcga cattggggtc acttcatcaa gcgtcctgtt
       61 actccggttg atattggaca aggtccctat gcgactgtta aagtcgagga cgaatcggga
      121 cgcgctgtgc cagctcaaat cgtagacggg aaactatttc caaacggtta tcggctcaag
      181 gtgcagttcg tagccgagtc tgttcccggt tgtggtttac gcacgtacta tgtgactccg
      241 gaagagtcag gcagtgcagg gtctgatggg atcgttgtca gagatacgac catcgagaca
      301 cccttgctca aggttgttgt ggatgcgaag acaggacacc ttctgcgcat ctatgacaaa
      361 aggcaacgga gggatgttct tggaaagaac actcgagcga acgtcctgag gctttgtatg
      421 gaagcccctc acggaatgac tggctgggtg atcggtccag tcaactctgt tgaggatctc
      481 gacaaggcag aatcggttca agtggttgag cagggtccgg ttcgcgccgt aatccaggtt
      541 gaacgcaaat gggatcgctc aacgttcaca caacgcatca tcgtttacaa agatttgcca
      601 cgagtggatt ttgaactgga cgtccgatgg ttccaattgg gcaatcatga ggttggtgtt
      661 cccatgctca gagttgcctt cccactgaac gtcaccaatg gaagattcag ttgcgatact
      721 ccatttgcca cagtcgaaag acccacgaca ggccaggaag tgcccgcgca gaaatggaca
      781 gacctttctg gccccaatgg tggagcggca cttttgaaca acagcaagta tggtcatcgc
      841 tgtgaaggga agactcttga gatgacgcta ttgcggtcat cctacgatcc cgatctgtac
      901 cctgatcagg gtccgcatca tattcggtat agtctatttc cacatgccgg gagttgggat
      961 gaggcagccg ttgatcttga ggggacttcc ttcaacttcc ctctgctcgc gatcgaagcg
     1021 actccgtcgg agcagggcga ccttgcctca ggaacgagtt tcctgacact caatcccggc
     1081 aacctcttgc tgtcagcgat caagagagcg gaagacggcg cttcgcttgt tgtgcgattc
     1141 tatgagtcgc aggggagaaa atcccatgca cttctcagat tcaaccgagc cgtgaagtgt
     1201 gtgacacggg tgaatctcct ggaagagccc ttggatggag tgcccgatgc gacgctgtcc
     1261 ggtaagaaag tgatggttcc ggtcaagccg catgagattg tatctcttcg ggtagacttc
     1321 tgagcaggtt gacacctgca gacgcacagg ccatgccaag attcttaggt gcttgcttgg
     1381 tagatgacgc gcttcttcca ggactgcgcg ttgccaagac gtggcatgct cagcaggcaa
     1441 ctgcctagaa gttgaagttg gcgatgtttg cggcagtgaa acgagttggt ggaccgagaa
     1501 gcacttcgtt tcctttgacc tgaattttg
//
LOCUS       DAOQUC010001143         1529 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_112895_length_1529_cov_3.528494, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001143 DAOQUC010000000
VERSION     DAOQUC010001143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1529
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112895_length_1529_cov_3.528494"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1529)
                     /locus_tag="PKH07_16320"
     CDS             complement(<1..>1529)
                     /locus_tag="PKH07_16320"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNT36553.1"
                     /translation="QYQMGLYSESLQQTRRLLTLAPGERVAEIRVALCLFQLGNLDKA
                     REEFEALLAKYPGSYEVRAGLGSCLVHKARSADQQDRDVLLKRAEEEISQSLQIRPGS
                     LEGLLALAEVQFLSGQIQEASSTAAQVIALDAESTFAADLMGQAAMELGAYAQAEEFF
                     NQALRFRRNTTPTVSAMIRDHLGVAGYCQNKLQVSYDEWNRANQIDPSYHPSYDNNAC
                     LAMLRQKNNRSIELLTTGMQLLGFGSTTEISSSAVPSFQQKTNLLLRSACARLRLEQY
                     NDAASILEQLTRLAADQPFGFFELGNVQYLRGRREDSVRAYEQAWSLAVDVPVVGYNF
                     GSLLLEQGSYTRSQEVLSRVVQIDPSNLDAHLNLGDALLNARNVKGASEQFQKASEID
                     TGNALPLIRMGQTMELVGDLRRAEQAYLKGITLDPSNGRTYFFLGLLRRRLSGTDSLA
                     QAEKDLAKAIELEPSNDTARTELGYVFLKANKLMEAEEQFKTVLENPLYASYDKAYNG
                     LG"
BASE COUNT          356 a          418 c          402 g          353 t
ORIGIN      
        1 cccagaccgt tgtaggcttt gtcgtacgag gcatagagcg ggttctcaag aacggtcttg
       61 aactgctctt cagcttccat caacttgttc gctttgagaa agacgtatcc cagttcagtt
      121 cgcgccgtat cattggaagg ctccagttca atagctttgg caaggtcttt ctcggcctga
      181 gcaagactat cggtaccgga caacctgcga cgcaacaagc ccagaaaaaa gtaagttcgt
      241 ccgtttgagg gatcgagtgt gatgcctttc agataggctt gttcggctct cctgagatcc
      301 ccgacgagtt ccatggtttg tcccatccga atcagtggaa gagcattgcc ggtgtcaatc
      361 tctgaggctt tctggaactg ctccgaagca cccttgacat tgcgggcgtt cagaagcgcg
      421 tcccccaggt tgaggtgggc atcgagattg gacggatcaa tctgaaccac tcgcgatagc
      481 acttcctgcg agcgagtata tgagccttgc tccaggagca aactgccgaa attgtatccc
      541 acgactggaa cgtccacagc caggctccac gcctgctcat aggcgcgaac agaatcctca
      601 cgtcttcccc gaaggtactg aacgttcccc agctcaaaga aaccgaaagg ttgatccgct
      661 gccaggcgag tcaactgttc gagtatgctt gcagcatcgt tgtactgttc cagccgaagt
      721 cgagcgcagg cacttctgag caacaaattc gtcttctgct gaaaggaagg aacagcactg
      781 gacgaaatct ccgttgtgga gccaaatcca agcagttgca tccctgtggt caagagttcg
      841 atcgaacggt tgttcttctg gcgcagcatt gcaaggcacg cattgttatc gtatgaggga
      901 tggtaggatg gatcgatctg gttggctcgg ttccactcgt cgtaggacac ttggagtttg
      961 ttctgacaat accccgctac accaagatga tcgcggatca ttgcagaaac ggtcggagtg
     1021 gtgttgcgcc gaaatctcag cgcctggttg aagaattcct cggcctgagc ataagctccc
     1081 aactccatcg ctgcctgacc catcaaatcc gctgcaaaag tggattcggc atccagtgca
     1141 atcacctgtg cggcagtgga actggcttcc tgaatttgac cggacagaaa ctgtacctct
     1201 gccaacgcca agagtccctc aagggaaccg ggacgaattt ggagcgactg actgatttct
     1261 tcctctgcac gcttcagaag aacatctcgg tcttgttggt cggcagaacg tgccttgtga
     1321 accagacagc tgccaagacc cgcccggact tcatagctgc caggatactt cgccaatagg
     1381 gcttcaaact cttcccgagc cttgtcaagg tttcccagtt gaaagaggca gagtgcaacc
     1441 cgtatctcag caacccgttc gcccggcgca agagtcagca agcgtcgagt ctgctgcaga
     1501 gactctgagt agagtcccat ctggtactg
//
LOCUS       DAOQUC010001144         1528 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_113039_length_1528_cov_2.873727, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001144 DAOQUC010000000
VERSION     DAOQUC010001144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1528
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113039_length_1528_cov_2.873727"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1528)
                     /locus_tag="PKH07_16325"
     CDS             complement(<1..>1528)
                     /locus_tag="PKH07_16325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015752789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNT36554.1"
                     /translation="CYHPDQGAPAKAVRLLVEVTDALQKEKAASLVAAEHYMKFCDEF
                     LVGQMASLDKEIQEALQTSASSEVGVQSTITAEKLKERVTKTVLCNKVIDLGNLIRIG
                     TWQAIASRDPELFVQTEKMFEQVNKTLDDLKAITVQEVNLKQIEECRAAGKEYEGCMD
                     RFLSNWLSREDIAKKRLAAATTVLEAAQATSEFNMEETEKATEHAAEALGMASTVMII
                     GLSVGTVVGILLAFFITRSITGPLRRVIDGLNAGAEQTASAAGQVSQSSQQMAEGASE
                     QASSLEETSSSLEELTSMTKQNADNANQAKSLAGNANTSAQKGAEAMTKMSTAIADIK
                     KSADETAKIVKTIDEIAFQTNLLALNAAVEAARAGDAGKGFAVVAEEVRNLAQRSAEA
                     AKNTALMIEGSVKNAENGVQISQEVANALAEIAEVAGKVTSLVTEVAAASNEQTQGLN
                     QINTAVAQMDQVTQANAANAEESASASEELNAQAAEMKRMVQDLVAMVGGAGSAGAGT
                     V"
BASE COUNT          270 a          471 c          383 g          404 t
ORIGIN      
        1 accgtacctg cgcccgcgga accggcacca cccaccatgg ccacaagatc ctgaaccatg
       61 cgtttcatct ccgcagcctg agcgttcaac tcttcggacg ccgaagccga ttcctcagca
      121 ttggcggcat tggcttgcgt cacctgatcc atctgtgcca ctgctgtgtt gatctgattc
      181 agtccctgtg tctgttcatt gctcgccgcg gcgacctctg tgacaagcga ggtcaccttg
      241 ccggcaactt ctgcaatctc ggcaagcgca ttggccactt cctgactgat ctgcacacca
      301 ttttcagcat tcttgaccga cccttcgatc atgagagcag tattctttgc cgcttcggcg
      361 ctacgctgtg caaggtttcg aacctcttcc gcgaccactg caaagccttt gcccgcatca
      421 cccgcacgtg cagcctcaac tgcagcattg agcgcaagca ggttggtctg gaaagcaatc
      481 tcatcaatgg tcttgacgat ctttgccgtt tcgtccgctg atttcttgat gtcggcaatg
      541 gcagtgctca ttttcgtcat cgcctcggcg cccttctgag cgctcgtgtt ggcattgccc
      601 gccagactct ttgcctgatt ggcgttgtcg gcgttctgtt tggtcatcga ggtcagttct
      661 tcgagtgaag aagaggtctc ctcgagactg gatgcctgtt cgctggcacc ttccgccatc
      721 tgctggctgg actgggaaac ctgtcccgcg gccgatgccg tctgctccgc tccggcattc
      781 aaaccatcaa taactcgtcg aagcggtcct gtgattgaac gtgtgatgaa gaacgcaagc
      841 aggattccca ccacagtccc cacgctcagc ccaatgatca tcacagtgga ggccattccc
      901 agtgcttccg cggcgtgttc tgtcgccttc tccgtctctt ccatgttaaa ctcggacgtg
      961 gcttgtgccg cttcgagaac ggttgtcgca gcggcaaggc gtttcttggc tatgtcctcg
     1021 cgggagagcc agttgctcaa gaaccgatcc atacagcctt cgtattcttt gcctgccgct
     1081 cggcactctt cgatctgctt caggttgact tcctgaacgg tgattgcctt cagatcgtcc
     1141 aaggtcttgt tcacttgttc aaacatcttc tccgtctgaa caaagagttc gggatcgcgt
     1201 gacgcgatgg cctgccaagt gcctattcga atcaagttgc ctaaatctat gaccttgttg
     1261 cagagtaccg ttttggtcac tcgttcttta agtttctctg cagtgatggt tgactggacg
     1321 ccgacctcgg agctcgcgct cgtctgcaag gcttcctgga tttccttgtc cagtgacgcc
     1381 atctgtccca ccaagaactc gtcgcagaat ttcatgtaat gctctgcggc caccaaagaa
     1441 gcggcctttt ccttctgcag ggcatcggtc acttccacca gcagtcgcac cgccttggcc
     1501 ggtgctccct gatcggggtg gtagcagt
//
LOCUS       DAOQUC010001145         1527 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_113187_length_1527_cov_2.347147, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001145 DAOQUC010000000
VERSION     DAOQUC010001145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113187_length_1527_cov_2.347147"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1019)
                     /locus_tag="PKH07_16330"
     CDS             complement(<1..1019)
                     /locus_tag="PKH07_16330"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNT36555.1"
                     /translation="MSGRGAVFAFAVIVLILGSFFLYPLYKTVRGGFFDDDGHFTLEF
                     LKEVFRNPIYLEGLWNSFRLAVATTLVVLCLSVPLAFLSSRFLFPGKSWLAGIVLVPM
                     ILPPFVGAIGLRQFWGQMGVLNAILFRLGICSGRPIDWLGINQFWGVVIAQALHLYPV
                     MYLNATAALSNIDPAMDEAARNLGAGRLRRLFGIMLPLMMPGLFAGGTIVFIWAFTEL
                     GTPLMFDFNRVSSVQVFSAIKEVGDNPFAYVLVVVIMFSSVCTFLFGKFIAGRHSQVV
                     ATKAARASQEKRLVGSRAWMATLLFAGVSLLALIPHTCVVLMSLSRDWYSSILPSQWT
                     TEHYRA"
     gene            complement(1021..>1527)
                     /locus_tag="PKH07_16335"
     CDS             complement(1021..>1527)
                     /locus_tag="PKH07_16335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HNT36556.1"
                     /translation="QRFIVFTLSMDGQKLWGFRAGTPGGPSKYTLHRLPIRRDFYVSE
                     FMPYRSHPGENPFTLAESFTYHGEWTGKLFGITRLLIQSMCLNTGDELRLAWRAVLDA
                     GGPDASPRAMRFFEALPENASLERIAETNSEMRRKADEVRIVRLWGAFFQQQYRLARE
                     AALETKGI"
BASE COUNT          416 a          438 c          383 g          290 t
ORIGIN      
        1 gctcgatagt gttcggtcgt ccactggctg ggaagaatgc tggagtacca gtcacggctc
       61 aggctcatca gcaccacaca agtgtgcggg atgagcgcaa gcaatgagac gcctgcaaaa
      121 agaagtgtcg ccatccatgc tcggcttcca acaagacgct tctcttgact cgctcgagcc
      181 gctttggtcg caacaacctg gctgtgtctt ccagcaatga acttcccaaa gagaaatgta
      241 cagacagaag agaacataat tacaacaacc aacacgtagg caaacgggtt atctccgacc
      301 tccttgatgg cgctgaagac ctgaacgctg ctcacacggt tgaagtcaaa cataagggga
      361 gtgcccaatt ccgtgaacgc ccagatgaag acaatcgtgc caccggcgaa aagtccgggc
      421 atcatcagag gcaacatgat cccgaacagg cggcgcagcc gacccgcgcc cagattcctc
      481 gccgcttcat ccatcgccgg atctatgttc gaaagagctg cggttgcgtt gagatacatc
      541 actggataca gatgcagggc ttgagcaatc acaacgcccc aaaactgatt gatgccaagc
      601 cagtcaatgg gtcgtcccga gcagatgcct agtctgaaga gaatcgcgtt cagcaccccc
      661 atctgtcccc agaattgccg aagtccaatc gccccgacga aaggcggcag aatcatcggt
      721 acaaggacaa tccctgccag ccacgactta ccgggaaaca gaaatcgcga actgagaaac
      781 gccaacggaa ccgaaagaca aaggacaacc agagttgttg ccacagcaag acgaaacgag
      841 ttccaaagcc cttcgagata gatgggattg cggaagactt ccttgaggaa ctccaaagtg
      901 aaatgcccgt cgtcatcaaa gaaaccaccg cgaacggtct tgtataaggg atagagaaag
      961 aagcttccca gtataaggac gatcaccgca aacgcgaaaa ctgctcctct gcctgacatc
     1021 tcagatcccc ttcgtttcta acgccgcttc acgcgcaagt cggtactgct gctggaaaaa
     1081 ggctccccag agtcgaacga ttctgacttc atccgctttc cttcgcatct ccgaattggt
     1141 ctccgctatg cgctccagac tggcgttctc cggcaacgct tcaaaaaaac gcatcgcccg
     1201 cggggaggca tccggccctc cagcgtcgag gaccgccctc cacgccaaac gcagttcatc
     1261 acctgtgttg agacacatgc tttggatcag aagcctggtg atgccaaaca gtttccctgt
     1321 ccattcccca tggtaagtga aggactctgc cagcgtgaat ggattctcac caggatggct
     1381 tctgtacggc atgaattcag aaacatagaa gtctcggcgg atgggaagcc ggtggagtgt
     1441 atacttggaa gggcctcccg gtgtgccagc tcgaaagccc cagagtttct gaccgtccat
     1501 tgacaacgta aagacaataa aacgttg
//
LOCUS       DAOQUC010001146         1526 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_113251_length_1526_cov_3.493542, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001146 DAOQUC010000000
VERSION     DAOQUC010001146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113251_length_1526_cov_3.493542"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            12..215
                     /gene="rpsU"
                     /locus_tag="PKH07_16340"
     CDS             12..215
                     /gene="rpsU"
                     /locus_tag="PKH07_16340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HNT36557.1"
                     /translation="MSAIEVYVDENETIDSALRRFKRQCEQSGLKAEIRRHEYYEKPS
                     VRKKRKMENAKRNQRRRMRMHNG"
     gene            556..1407
                     /locus_tag="PKH07_16345"
     CDS             556..1407
                     /locus_tag="PKH07_16345"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HNT36558.1"
                     /translation="MPRETSYDDDRLVSVYLKEIGDIPLLTAEEEIDLATRARKEDTA
                     ARRRLILANLRLVVSTARKYNNKSVALLDLIEEGNIGLIKAVERFDPAKGCRFSTYAV
                     WWIRQAINRAILNQTSLVRLPSHKAENVNKVRKIFNSLQQELGRAPNEEELLEKVPLS
                     KKEAREAVRLYFMPSSLEYLTGLEDEGAYRADLEDTSIIPPDVQIEMRTRNERVLSLL
                     GSLQGREKQILRLRFGFEDGKPYTLEEIGEKMKLTRERIRQLEHRGLARLRTLARFQQ
                     GLDDSCL"
BASE COUNT          400 a          365 c          447 g          314 t
ORIGIN      
        1 ggtgttaggt tatgtccgcg atagaggtct acgtcgatga aaatgagacg atagacagcg
       61 ctttgcgtcg cttcaagcga caatgcgagc aatcaggcct caaagcagaa attcggcgtc
      121 atgaatacta tgaaaagccg agcgttcgta agaaacgcaa gatggaaaac gccaagcgca
      181 atcagaggag gcgaatgagg atgcacaacg gctgagaatg ccgtttgtct tcctgaacag
      241 gttggctgcc tgtatcccat cccgagcagg tgagcaccct tggacgcttg ctcgtcgaaa
      301 tgacacgctt cttctggact gagtgtcatc caggagcaac tgaccgcttg ttgatgcggt
      361 tgctcgaaaa aggaactggc agtttcttag gactcgaaaa gaaaccgagt gcctctgaag
      421 tctggttttt gggagagtgg ttcagaacct gaacgcgcag gcgcagaaga cgacggcgca
      481 ggcgaagatc aggtcgaaag gaacggtggt tccccgagga accgccctca tccgtaagat
      541 gacggagcca gtacggtgcc tcgagagacg agctatgacg atgatcgcct ggtttcggtt
      601 tatctcaagg aaatcgggga cattccgctc ctgacagcgg aagaagaaat tgatctcgcc
      661 actcgcgcaa ggaaagagga cacagcagcc cgaaggcggt tgatactggc gaatcttcgc
      721 ttggtggtca gtaccgcacg aaagtacaac aacaaaagtg ttgcccttct tgatctaatc
      781 gaggagggca acattggttt aatcaaggcg gtggaacgct ttgatccagc caagggatgc
      841 cgcttcagca cgtacgcggt ctggtggatc cggcaggcga tcaaccgtgc gattctgaac
      901 cagaccagcc tggttcgact tccttcgcat aaggcggaaa acgtcaacaa ggttcgtaag
      961 atcttcaact cacttcagca ggaactgggt cgtgcaccaa acgaagagga actccttgag
     1021 aaagttcccc tgtccaagaa agaagcgagg gaagcggtca ggctttattt catgccttct
     1081 tcgttggagt atctgacagg gcttgaagat gagggagcct atcgggcgga cttggaagat
     1141 acttcgatca ttcctcccga tgttcagatc gaaatgcgca cccgaaatga aagagtcctg
     1201 agcctgctgg gatctcttca gggcagagag aagcagattc ttcgtctgcg ctttggcttt
     1261 gaggacggca agccttacac cctggaagag atcggagaga aaatgaaact gacccgggag
     1321 cgcatccgtc agttggaaca ccgcgggctg gcacgactcc gaaccttggc gcgcttccag
     1381 cagggcttgg atgactcgtg tctctagtat tgcgttctga aagtagagtt gcatagagca
     1441 gctgctcagt ctgggagaca aggaagtctg aagaaccccc gcaacccctc ctattaaggg
     1501 gaataagggg gattaggacg accgga
//
LOCUS       DAOQUC010001147         1524 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_113496_length_1524_cov_3.773996, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001147 DAOQUC010000000
VERSION     DAOQUC010001147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1524)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1524)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1524
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113496_length_1524_cov_3.773996"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..618)
                     /locus_tag="PKH07_16350"
     CDS             complement(<1..618)
                     /locus_tag="PKH07_16350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HNT36559.1"
                     /translation="MSEKLPSYIQMAKPNPPENRAPWYKNTAPTYAGIFLWFVFWSGM
                     ASGGAKFAGGTLSQGVGVALLGLVLSALICHFLFYKIPGLLGMKTGLPLYVVGSSTYG
                     ANGGFLMPGFLMGVLQFGWLGVNAFFASILLKPLFGQGDLVITQKIIAIVWAALAAFL
                     GLKGIQYVAKVATYLPLIPFVILLVLLAKTLGGLGGFNAAELVQNA"
     gene            886..>1524
                     /locus_tag="PKH07_16355"
     CDS             886..>1524
                     /locus_tag="PKH07_16355"
                     /inference="COORDINATES: protein motif:HMM:NF015604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046559 - alpha-glucuronidase activity
                     [Evidence IEA];
                     GO_process: GO:0045493 - xylan catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucuronidase family glycosyl hydrolase"
                     /protein_id="HNT36560.1"
                     /translation="MLRIAIIVFAFMLLPFRVQAKEITLVKSGKTAYTIIVASDASPS
                     ELHAARELQDFVAQMSGAQMPILTDAYPLPEKAVVLGENRWSQTLVPDLNVKELAKEG
                     YIQKTVGEHLVITGGKPRGTLFGVYGILEDHLGCRWFTPDCSRIPKSKTIVLSELDER
                     IIPRLEYRDPFYSDGFDGDWVLRNRMNSSRAAGVESKGGKVQFSDLAFCHTFF"
BASE COUNT          378 a          405 c          380 g          361 t
ORIGIN      
        1 agcattttga accagttctg cagcattaaa gccgcccaaa ccacccaacg tcttcgcaag
       61 cagtacaagc agaataacaa atggaatcaa aggaaggtac gtcgccacct tggcaacata
      121 ctgaattccc ttcagcccca aaaacgccgc cagcgctgcc cagacgatgg caataatctt
      181 ttgagtgatg acaagatccc cttggccgaa aagcggtttc aatagaatgg atgcgaaaaa
      241 ggcgttgacg ccaagccacc cgaactgaag aactcccatg aggaagccag gcatcagaaa
      301 gccaccgttt gcgccatagg tcgaggaacc aacgacatag agcggaagac ccgtcttcat
      361 gcccaggagc ccagggatct tgtagaaaag gaagtgacag atcagagccg acagaaccag
      421 ccccaacagg gcaactccaa caccttgaga cagcgtccct ccggcaaatt tcgcaccacc
      481 gcttgccata ccgctccaaa agacgaacca gaggaagata ccggcatacg ttggcgcagt
      541 gttcttgtac cacggtgctc gattctcagg cgggttcggc tttgccatct gaatgtaact
      601 cggcagtttc tcgctcatac ttctgttctc cttttgaatg aaatacgtct ctcgccggtc
      661 agctcatcag ccgactcaat ggttcgtagc atacaccaaa tgacaaagtc ggcaaagagt
      721 tttctccgaa cgagcagatc aaggtgtgta gaacaagaat gctttctgat tcgttggagg
      781 cattttgttg gtcatcccca tttgcgcttt cgctcccatc ccgctagtct ctttctcaat
      841 gcgttcttgc gcacgatgtt gaagtcgcat tcagggaggc aaataatgct caggattgcc
      901 attattgttt tcgcgttcat gctgctgcct ttcagagtac aggccaagga gatcaccttg
      961 gtcaagtccg gcaaaactgc ctatacgatc atcgttgctt cggatgcttc gccctcggaa
     1021 cttcacgcgg cgcgagagct gcaggatttt gtcgctcaga tgagtggtgc tcagatgccc
     1081 atcctgacgg atgcataccc gctaccggag aaggccgtcg ttcttggtga gaacaggtgg
     1141 tctcagactc ttgtgccgga tttgaacgtc aaggaacttg ccaaggaagg atatattcaa
     1201 aaaaccgttg gtgagcatct tgtgatcact ggaggcaagc cccgcggaac gctgttcggt
     1261 gtgtatggca ttcttgagga tcatctgggt tgccgatggt tcacaccgga ctgcagcagg
     1321 attcccaagt ccaaaacgat tgtactctcc gaactggatg aacgcatcat tcctcgactt
     1381 gagtatcgtg atcctttcta ttctgatggc tttgacgggg actgggttct ccggaaccgc
     1441 atgaactcct cgcgcgccgc cggtgttgag tccaaggggg gcaaggttca attcagtgat
     1501 cttgcttttt gtcacacatt tttt
//
LOCUS       DAOQUC010001148         1523 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_113633_length_1523_cov_3.568801, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001148 DAOQUC010000000
VERSION     DAOQUC010001148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1523
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113633_length_1523_cov_3.568801"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(188..1174)
                     /locus_tag="PKH07_16360"
     CDS             complement(188..1174)
                     /locus_tag="PKH07_16360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011943376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNT36561.1"
                     /translation="MQVIATTGNEDVALVYIAKMSGGRPIEFAESIQPPIPREKKWVL
                     LVSTMFGCPIGCQICDAGGCYQGTLTKEELIEQLDFLVYRRFPDGQVPVKQFKIQFAR
                     MGEPSLNPAVLDVLDELPKRYRAPGLMPSLSTVAPLGRQEFFERLLANKQACYPQGRF
                     QLQFSIHTTDVELRDKIIPCGKWTFENIAEYGERFYAPGDRKISLNFALICESPVDPK
                     VLLRYFDPGKFLIKLTPLNPTYRARENKLIQYVSPVGGLDNCEMAQELRDAGFEVIVS
                     VGELEENCIGSNCGQYIRRHLLATEPIDDAYTYSIRNAMSEPHGSESDSTIM"
     gene            complement(1183..>1523)
                     /locus_tag="PKH07_16365"
     CDS             complement(1183..>1523)
                     /locus_tag="PKH07_16365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36562.1"
                     /translation="VLWERSQASSQISYHARTTVSTLSETASGGIALQCAAPAGSINL
                     YADYLIGAIGREPQLDFVDTQLLERTAELEEHGSLYRIGDVRNGIFRQTSIAVGDGVL
                     AAMSICRQQE"
BASE COUNT          321 a          440 c          397 g          365 t
ORIGIN      
        1 gagcaaccag tcgcgaacag cttcgcccac ttctattcct atcgtcctgc ctcggtggtt
       61 ggtcagccgt ctaacgcccc ggttcagcgg cgggccgcgc agcggaccgt ccgctgcaac
      121 cggttgttag gcgatactct cttgatctca tcctggctct gtagaccagc atgttgccgc
      181 catactctca catgatagtg ctatcagatt ccgaaccgtg tggctcactc atagcatttc
      241 tgatgctgta cgtataggca tcatcaatcg gctctgtcgc taacaggtgg cggcgaatat
      301 actgaccgca gttgctcccg atacagtttt cttccagttc accgacactc acaatcactt
      361 cgaatccggc atctcgcagt tcttgtgcca tttcgcagtt gtcaagtccc ccaaccggac
      421 tcacatattg gatcagtttg ttctctcgag cgcggtaggt gggattcaga ggcgtgagtt
      481 tgatcaagaa cttgcctggg tcgaagtatc gcagcagcac tttcggatcc actgggctct
      541 cgcagatcag ggcaaagttc aggctaatct tgcgatcgcc gggagcatag aagcgctcgc
      601 catattccgc tatgttttcg aaagtccatt tcccacacgg aatgattttg tcgcgaagtt
      661 cgacatcggt ggtatgaatc gagaactgca attggaagcg cccctgaggg tagcatgcct
      721 gtttgttagc gagtaatcgt tcaaagaact cttgccttcc caagggagcg acggtggaaa
      781 gcgatggcat tagaccgggt gccctatagc gtttcggcag ttcatccaac acgtccagga
      841 ccgccgggtt caaggatggt tcacccatac gggcaaactg tatcttgaac tgtttgacag
      901 gaacctgccc gtcaggaaac cgacggtata cgaggaagtc gagctgctca atgagttcct
      961 ccttcgttag cgttccctga tagcaaccgc ctgcgtcaca tatctggcac ccaatcgggc
     1021 agccgaacat cgtcgagacc agcaagaccc atttcttttc gcggggaatc ggcggctgga
     1081 tcgattccgc aaactcgatc ggccgaccgc cactcatctt ggcaatgtac accaacgcga
     1141 catcttcgtt tcctgttgtt gctatcacct gcatccttgc ggctactcct gttgtcgaca
     1201 aatgctcatg gcagccaaca ctccatcacc gactgcgatt gatgtttggc gaaaaatgcc
     1261 gtttctgacg tcaccgatcc tgtagagcga tccatgttct tccaattcag cggtgcgttc
     1321 cagtaactgc gtgtccacga aatcgagctg tggctcccgg ccaatggccc cgatcagata
     1381 gtccgcatag agattgatcg agccagccgg cgcggcgcac tggagggcga tccccccgga
     1441 ggcggtttca ctcagcgtgg agacggtcgt tcgggcatgg tagctgatct gcgatgaagc
     1501 ctggctccgc tcccagagca cag
//
LOCUS       DAOQUC010001149         1523 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_113674_length_1523_cov_2.714578, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001149 DAOQUC010000000
VERSION     DAOQUC010001149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1523
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113674_length_1523_cov_2.714578"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..718
                     /locus_tag="PKH07_16370"
     CDS             <1..718
                     /locus_tag="PKH07_16370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36563.1"
                     /translation="DVNQQQNGGVWVLLGTYAFASGSSGYIRLDNGPAGASVVIADAV
                     KLELASPAGPTNTPTNTPTRTPTNTPTRTPTNTPTQPPTNTPTNTPTRTPTPATTPVE
                     RIIDDGDSGYTKSGTWSYSTCSTSYALNGDYDWANSTTGASTAWAKWTPNLTAAGSYK
                     VYVMFRSGSNRATDAPYTVYYNGGNQTIDVNQTINGGTWVLLGTFNFASGTAGYVQLN
                     NGPAQASKAIIADGVKFTPN"
     gene            complement(826..>1523)
                     /locus_tag="PKH07_16375"
     CDS             complement(826..>1523)
                     /locus_tag="PKH07_16375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36564.1"
                     /translation="LEFSRDVEMMKGGYGDNYYMQMAANIRRFKGMPPAPKYSAPKSI
                     DISDSFEQWDDVLPEYRDHTMDTEPRDHPGCGGLHYTVDTGRNDFQTLKVTYDAHKLY
                     FHAKTRNRITPSTDPNWMWLLIDVRGDGLPDWEGFSFIVNRDRLSGNESSVEICTGGF
                     EWKPLGRADFRCEGDQIHIAVPRSLLRIGSSPFVIEFKWFDNVREPGNILDAYVNGDT
                     APDGRFRYRFEAF"
BASE COUNT          397 a          440 c          357 g          329 t
ORIGIN      
        1 ggacgtcaat cagcaacaga acggtggcgt gtgggttctt ttgggaacct atgcctttgc
       61 ctcaggcagc tccggataca ttcgactcga caacggtccg gcgggcgctt cggtcgttat
      121 tgccgatgca gtcaaactcg aactcgcttc accagcaggc ccaacgaata cgccaaccaa
      181 tacaccgacg aggactccga ccaacactcc aactcggact ccaacgaata caccgacaca
      241 accgccaaca aatacaccta caaacacacc gacacgaaca ccaactcctg caacaacgcc
      301 tgtcgagcgc attattgatg acggtgattc cggatatacg aagagcggca cttggtcata
      361 ctcaacatgc agtacatctt acgcactcaa cggcgattac gactgggcaa acagcacaac
      421 aggtgcctcg actgcttggg caaaatggac tcccaacctg acggcagcgg gaagttacaa
      481 ggtctacgtc atgttccgat cgggaagcaa ccgagcgacg gacgccccgt acacggtgta
      541 ctataatggt ggcaaccaga ctattgacgt gaaccagacg atcaatggtg gaacatgggt
      601 gctcttggga acgttcaact ttgcctcagg tactgcaggc tacgtccaac tcaacaatgg
      661 acccgcgcaa gcaagcaagg caatcattgc cgacggagtg aaattcactc caaactagca
      721 aatgctgaac ttgggggata tcttccccaa cacaatcggc tcaaacgaag gcagcggact
      781 gatcggtcag tccgctgctt ctctttccct caccgaaacg tagtctcaga acgcctcaaa
      841 gcgataccga aaacgtccat cgggtgcagt atccccattc acataggcgt caagtatgtt
      901 tccaggctct ctaacgttgt caaaccattt gaactcaatc acaaatggac tgctcccgat
      961 acgcaggagc gacctgggaa cggcgatgtg aatctgatct ccttcgcaac ggaagtctgc
     1021 cctgcccaga ggtttccact caaagccacc ggtgcatatc tcaacggagg actcattccc
     1081 gctcaatcta tctcgattca caatgaagct gaaaccctcc cagtcgggca gaccgtctcc
     1141 ccgaacgtca atcaggagcc acatccaatt cgggtctgta ctcggagtga tcctattcct
     1201 tgtcttggca tggaaataga gtttgtgcgc gtcgtaggtc actttgagag tctgaaagtc
     1261 attgcgtccc gtatcaaccg tgtagtgcaa accaccgcag cctggatggt ctctcggttc
     1321 tgtgtccata gtatggtctc gatattcagg aagtacgtca tcccactgct caaaggaatc
     1381 tgaaatgtct atgctcttgg gggcagagta ctttggggca ggtggcattc ctttgaaacg
     1441 gcgtatattg gctgccatct gcatgtagta attgtcgcca tatccaccct tcatcatctc
     1501 aacatcccga ctgaactcca ggt
//
LOCUS       DAOQUC010001150         1521 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_113910_length_1521_cov_3.036153, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001150 DAOQUC010000000
VERSION     DAOQUC010001150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1521
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113910_length_1521_cov_3.036153"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1398
                     /locus_tag="PKH07_16380"
     CDS             <1..1398
                     /locus_tag="PKH07_16380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36565.1"
                     /translation="LSPVWMALLAGLLFALMTVLYLVHRNPWRKDLGWMPAFVLLFSP
                     CFVFWSGSGLETAFFTFLLAVAVLRFIRECRSIEKCRAGESLLPPHFTSGVLLGLACL
                     TRMEGVLLLTVNLATLIILLRRGKIQAWLSVVWHLVSFGTVFGVWFLWHWWYYSHPFP
                     NTYYCKVWGGGYELWLRGLRHVTEFSLATICLPVFSVLSFVLSRDWRERYLAATILVL
                     IGGVVRVGGDHFALYRFLVPVLPLLALLVPLWFQRLLAPRSREDESRSPSRPLPSAPL
                     QDSFSFKAAFPRWRSTAAFALYLVISATVCLLSFENHRSARLILEGTRGWAKTGRWFA
                     FQTPPDESIATQAIGAIGYYSERSIIDILGLVDEHIAHLPVRVGRGFPGHEKHDAVYT
                     LSRNPTYIVLGHSGRVPLKRDRQTVVDIIFQGSDFLRGEQEIIASPGFDERYEFESIA
                     LGDGTFIECFRLRDR"
BASE COUNT          256 a          405 c          421 g          439 t
ORIGIN      
        1 ctctcgcccg tctggatggc tctgcttgcc ggtctgttgt ttgcactgat gacagtgctg
       61 tacctcgtgc accggaatcc gtggagaaaa gatctcggct ggatgcctgc gtttgtcctg
      121 cttttctcgc cgtgtttcgt tttttggtcg ggaagtggac tggaaaccgc ctttttcaca
      181 tttctgctcg ccgtcgccgt gcttcgtttc attcgggagt gtcgttccat tgagaaatgc
      241 agagcgggag agtcgctact accccctcac tttacgtccg gcgtcttgct gggtcttgcc
      301 tgcttgacgc gcatggaagg ggttcttctg ctcactgtga atcttgccac actgataatc
      361 cttcttcgcc gcggcaagat acaggcctgg ctatctgtgg tgtggcatct tgtctctttt
      421 gggactgtct ttggagtttg gtttctctgg cattggtggt actacagcca tcctttcccc
      481 aacacctatt actgcaaggt ctggggtggc ggttacgaac tctggttgcg tggtctgcga
      541 catgttacgg agttctccct ggcaaccatc tgccttcccg tcttttcggt tttgtcattt
      601 gtgttgagtc gcgactggcg tgagcggtat ctggcagcga ccattcttgt gctgattggc
      661 ggagttgttc gcgttggcgg cgaccatttt gctttgtacc gattcctggt tcctgttctt
      721 ccattgcttg ctctgctggt gcctctgtgg tttcagcggc tcttggcgcc gcggagtcga
      781 gaagacgagt cgagatcacc atccaggcct ctgccttctg cccctttgca ggactcgttc
      841 tcattcaagg ctgcttttcc tcgttggcgc tccaccgcgg cgtttgccct gtatcttgtg
      901 atttcggcta cggtgtgcct gttgagtttc gagaaccatc gttcagcacg tctcatcctc
      961 gaaggcaccc gtgggtgggc aaagacgggc agatggtttg ccttccagac gccacccgac
     1021 gaatccattg ccacacaggc cattggagcc atcggatact actctgaacg cagcatcata
     1081 gacattcttg gtcttgtaga tgaacacatc gcacacttgc ccgttcgggt gggtcgtgga
     1141 ttcccaggtc atgagaaaca tgatgcggtc tatacccttt cgaggaatcc cacgtacatc
     1201 gttctgggtc attcagggag agtcccactc aagagggata gacagacggt ggtagatatc
     1261 atcttccaag gcagtgattt tctcagaggt gagcaggaga ttattgcgtc gcccggattc
     1321 gatgagcgat atgagtttga gtccattgcc ctcggcgatg gcactttcat cgagtgcttc
     1381 agattaagag accgatagcg gtgtctcagc cgcagaacgc aacgcagact cgagtgtcga
     1441 agcgttccac tctgttcgga gagtcctccg aagtgcgtag acatctgtcg ttgcgggtgg
     1501 caacactgta tccatgccct t
//
LOCUS       DAOQUC010001151         1521 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_113932_length_1521_cov_2.716235, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001151 DAOQUC010000000
VERSION     DAOQUC010001151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1521
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113932_length_1521_cov_2.716235"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(78..>1063)
                     /gene="rpoB"
                     /locus_tag="PKH07_16385"
     CDS             complement(78..>1063)
                     /gene="rpoB"
                     /locus_tag="PKH07_16385"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247879.1"
                     /note="DNA-dependent RNA polymerase catalyzes the
                     transcription of DNA into RNA using the four
                     ribonucleoside triphosphates as substrates; beta subunit
                     is part of the catalytic core which binds with a sigma
                     factor to produce the holoenzyme; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HNT36566.1"
                     /translation="KITKDSIAKAEKISIREIFLEDSEDIERLIIDYESRMEDVKHEF
                     EKKIEYIKTGDNLPPGVIKMVKVYVAKKKKLSVGDKMAGRHGNKGVVSKVLPVEEMPF
                     LDDGTPVDIILNPLGVPSRMNVGQVMETHLGWAAQKKGKLVASPVFDGATYEDIVEEL
                     SSCDLPETGKATLYDGRSGEPFDQPVTVGHIYMMKLNHLVDDKIHARCIGPYSLVTQQ
                     PLGGKAQFGGQRFGEMEVWALEAYGSAHTLQELLTVKSDDITGRNKIYEAIVKGQDAK
                     PPGLPESFYVLIKELQSLGLGIEIESEEDVLGSPDDKAEMTTQEKLKILGF"
     assembly_gap    1064..1073
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1074..>1521)
                     /locus_tag="PKH07_16390"
     CDS             complement(<1074..>1521)
                     /locus_tag="PKH07_16390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011420648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HNT36567.1"
                     /translation="RDIPNVGEEALRNLDEDGIIRVGAQVKPGDILVGKISPKGKVQT
                     SNVEKLLRAIFGKRAEDVRDISLKAHPGTEGTVVDVKVFSRKVRSGKAKGEDERKIKK
                     LTAEMDQAKNNLETALEQKSHQIAIGKILKQDLIDYNTGEVIALKGE"
BASE COUNT          326 a          412 c          366 g          407 t
ORIGIN      
        1 tctcttcgct atgcgctgtc aaccaaactg ccaaatactt catctctcgg acgaggcgaa
       61 atgccggatc cgtcaaacta gaagccgaga atcttcagct tttcctgagt cgtcatctca
      121 gctttgtcgt cagggctgcc caggacgtct tcttccgact caatctcaat tcccaagccg
      181 aggctctgga gttccttgat gagaacgtag aaggactcag ggagtcctgg tggcttggca
      241 tcttgcccct tgacaattgc ttcgtagatc ttattcctac cggtaatgtc gtcagactta
      301 acggtcagca actcttgcag cgtgtgagcg gagccgtatg cttccagtgc ccagacttcc
      361 atttctccga aacgctgacc accgaactgc gcctttccgc ccagaggctg ttgggtaacc
      421 aaagaataag gaccgatgca acgggcatga atcttgtcat caaccaagtg gttcagtttc
      481 atcatgtaga tgtgaccgac ggtaacgggt tggtcaaaag gctcaccact gcggccgtcg
      541 tagagagtgg ccttaccggt ttcgggaagg tcacacgagg aaagctcttc gacgatgtcc
      601 tcataggtgg ccccatcgaa aactggagaa gcaaccagtt tccccttctt ctgagccgcc
      661 caaccgagat gagtctccat aacctggcca acgttcattc gggagggaac gccaagcgga
      721 ttcaggataa tgtcaacagg cgttccatca tcgaggaagg gcatttcctc aacgggaagc
      781 acctttgaaa ccacaccctt atttccgtgg cgtccggcca tcttgtcgcc aacggaaagt
      841 tttttcttct tggcaacgta gaccttgacc atcttgataa cccccggagg aaggttgtca
      901 cctgtcttga tgtactcgat cttcttttcg aactcatgct tgacatcttc catacgggac
      961 tcgtagtcaa tgatcaggcg ctcgatatct tcactgtctt cgaggaagat ctcacggatg
     1021 gaaatcttct ctgccttggc aatgctatcc ttggtgatct tctnnnnnnn nnntctcacc
     1081 tttgagggcg atgacttcac ccgtgttgta gtcaatgaga tcctgcttca agattttgcc
     1141 aatagctatc tggtgagact tttgttccaa tgcggtctca aggttgttct tggcctgatc
     1201 catctcagcg gtcagtttct tgatctttct ctcgtcttca cctttggctt tgccggaacg
     1261 gactttgcgc gagaagacct tcacatcaac gacggttccc tcagttccgg gatgagcctt
     1321 caaagagatg tcgcggacgt cttcagcacg ctttccgaag atggctcgaa gcagtttctc
     1381 cacgttggat gtctggactt tcccttttgg ggaaatcttg ccaacaagaa tgtcgccagg
     1441 tttgacctgg gcgccaacgc ggatgattcc gtcctcgtca aggtttcgca gtgcttcctc
     1501 gccaacattt ggaatgtcgc g
//
LOCUS       DAOQUC010001152         1521 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_113969_length_1521_cov_1.952933, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001152 DAOQUC010000000
VERSION     DAOQUC010001152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1521
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113969_length_1521_cov_1.952933"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /locus_tag="PKH07_16395"
     CDS             <1..371
                     /locus_tag="PKH07_16395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36568.1"
                     /translation="VIINHRISPCQGPGDPTLDRKEIANLFASEAAAYCSGLGFYSWN
                     EMVDVHMASNKNTSEDRPDAVMPVTPEQHERLYEIVSEINRAYHGIYKQNLKKVRVPQ
                     SPLGDKEKSTVYWLLRESDR"
     gene            complement(421..1212)
                     /locus_tag="PKH07_16400"
     CDS             complement(421..1212)
                     /locus_tag="PKH07_16400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013403615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HNT36569.1"
                     /translation="MSFNVLMVGDIVGRPGRKILTEMLPRFRADYSIHFAVVNAENAA
                     SGKGITKSIAEDFLSLGADVLTLGNHVWDNKDILGSLDNERLLRPANYPPVGVPGHGV
                     FRCQLPSGVKITVVHLMGRVFMTPIDCPFRTMDKILEEVAPGEIVLVDFHAEATSEKK
                     ALACYLDGRVSVLCGTHTHVQTADEQILPNGTAFISDIGMTGPHAGIIGMRSKEILER
                     FLTQMPAKYEVGEGDEKFQALFVQLNEQTGKAVTVERINFDVQQD"
     gene            complement(1299..>1521)
                     /locus_tag="PKH07_16405"
     CDS             complement(1299..>1521)
                     /locus_tag="PKH07_16405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017796740.1"
                     /note="protein from Staphylococcus aureus has
                     phosphodiesterase activity against 2'-3'-cAMP and
                     2'-3'-cGMP; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HNT36570.1"
                     /translation="LEEVANSFSGVERSFAIQAGRELRILVEPDEIDDLESVKLAREI
                     RNRIEQEMEYPGQIKISVIRETRAVEYAK"
BASE COUNT          369 a          414 c          376 g          362 t
ORIGIN      
        1 acgtgattat caatcatcga atctctccgt gtcaggggcc tggtgacccg acgcttgacc
       61 ggaaagagat tgcgaatctc tttgcctcag aggccgcggc ctactgcagt ggtttgggtt
      121 tctacagctg gaatgagatg gtggatgttc acatggcatc caataagaat accagcgaag
      181 acagacccga tgccgtgatg ccagtgacac cggaacaaca tgaacggctc tacgaaatcg
      241 tcagtgagat caacagagcc tatcatggta tctacaaaca gaatctgaag aaggtcagag
      301 tgcctcaatc cccattgggt gacaaagaaa agagcactgt ctattggttg ctccgagaat
      361 ctgacagatg aggcggacgc ttcagactgt acggcaacgg agaatcaggg tcaaaggtgc
      421 tcagtcctgc tgaacatcaa agttgatgcg ttcaacggtc accgccttgc cagtctgctc
      481 gttgagttga acgaacaggg cttggaactt ttcatcccct tcacccactt catatttggc
      541 cggcatctgt gtcaggaacc gttccagaat ctccttgctt ctcatgccga tgatcccggc
      601 atggggcccc gtcatcccga tgtcagagat aaaagccgtt ccgttcggga gtatctgttc
      661 atcggccgtc tgcacatggg tgtgtgtgcc gcagagcacg gacacgcgtc cgtccagata
      721 acaggcgaga gctttcttct cggaggtcgc ttccgcatga aagtccacca gaacaatctc
      781 acctggagcg acctcttcga gaatcttgtc cattgttcga aaggggcagt caattggagt
      841 catgaagacg cgccccatca gatgaacgac cgtaatcttg acgcctgaag gaagttggca
      901 gcgaaagacg ccatgaccgg gaactccgac gggaggataa ttggccggtc tcaaaagtct
      961 ttcattgtcc agcgagccga gaatatcctt gttgtcccac acatgatttc ccagcgtcaa
     1021 cacatccgcg ccaagagaaa ggaaatcctc tgcgatggat ttggtgattc cctttcccga
     1081 tgcggcattc tctgcgttga caactgcgaa atgaatcgag tagtcagcgc ggaacctggg
     1141 cagcatttcc gtaaggattt tgcgcccagg tctcccaaca atgtccccga ccatcaatac
     1201 gttaaaactc atggcactca tccctcaaaa caaagcattg cctggtttcg gccaagcgtc
     1261 atcaacaaca tcaatccgtt tcccgcacca gttctctgct acttggcgta ttcgaccgcc
     1321 cgagtttctc gtatcacgga aatttttatc tgtccaggat attccatctc ctgctcgatt
     1381 ctattgcgta tctctcgagc gagtttgacg gactccagat cgtcaatttc atctggctcc
     1441 accaggattc gaagttctct tccggcctga atcgcgaagc tgcgttcaac tcccgagaag
     1501 gagttggcga cctcttcgag t
//
LOCUS       DAOQUC010001153         1519 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_114139_length_1519_cov_2.665984, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001153 DAOQUC010000000
VERSION     DAOQUC010001153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114139_length_1519_cov_2.665984"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..803
                     /locus_tag="PKH07_16410"
     CDS             <1..803
                     /locus_tag="PKH07_16410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36571.1"
                     /translation="FVFCYTSSIMNGVESPLRNPTMGYRLDLGEPGLLRTAPASQSAL
                     RVTAQETRNLMRLKGQALREGQIILYADITYTNSRSGSYPALSAGHTRVISREPRVKP
                     LERLSEFEQPSPAQSGQAPEETDHSQTPAGDMTQTPPAELSAPDRYPDLPDDPQQLLL
                     KEVAIRASIGRMEQRQQQIEQQAESSENVGQKAQARVEVDRLETRRDTMEEELRKVRM
                     AQVAATQQQLLDLIIQNADQAASVARLGSTLGMTNPQSAGSGGLDVVV"
     gene            884..>1519
                     /locus_tag="PKH07_16415"
     CDS             884..>1519
                     /locus_tag="PKH07_16415"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HNT36572.1"
                     /translation="MKLYEHEGKRLFALSGIPVPKGIVVASKDEARDAFRKIGPCVLK
                     AQVLWGSRAKEQAIIACDTQQSLEEAFDRLFARKVHEETVECLLVEEQLRISREYYLS
                     ITYRDRTPTVLASAHGGSEVVERLETDPDAVLVEPINILKGMDGETARTILNKAGFRD
                     GDAVQAAGILEKLYDFFIRNDALLAEINPLAKTSSGEWYAADAKVEIDDDAL"
BASE COUNT          365 a          390 c          453 g          311 t
ORIGIN      
        1 tgttcgtctt ttgctatact tcctccataa tgaacggtgt tgaatcgccc ctcagaaatc
       61 cgacgatggg ttaccgcttg gacttggggg agcccggact gttgcgaact gctcccgcaa
      121 gccagagcgc tttgcgcgtt acggcgcagg agactcggaa tctgatgcgc ttgaaaggcc
      181 aggcactccg agaggggcag atcatcctgt atgccgatat tacctacaca aacagcagat
      241 caggatcata tcctgccctg agcgcaggac acactcgcgt catttcccgc gagccgcgcg
      301 tcaaacctct tgaaagactg agcgaattcg agcagccatc tccagcacag tcaggacagg
      361 cacccgaaga gactgaccac tcacagactc cggctggcga tatgactcaa actccacctg
      421 ctgaactctc ggcaccggat cgctatcccg atctgcccga cgatcctcag caactcctgc
      481 tgaaagaggt tgccattcgg gcgtccattg gccgaatgga gcagaggcaa cagcagatcg
      541 aacaacaggc tgaatcttca gaaaacgttg gtcagaaagc acaggcgcga gttgaggtag
      601 accgcttgga gacgcgtcgt gacacaatgg aagaggaact gcgaaaggtg cgtatggctc
      661 aggtggcggc cacacagcag cagctgctgg acttgattat ccaaaatgca gatcaggcgg
      721 cttccgtcgc ccggttggga agcacgttgg ggatgacgaa ccctcagtct gctggttcag
      781 gaggtttgga cgtcgtcgtt taggtcgcag catggttgac tgctgcggat gggtgtgtcc
      841 acagaagcgt gggattgtcc acagaaagtt ttttggagag attgtgaaac tctacgaaca
      901 tgaaggaaaa cgtctgtttg ccctgtcggg tattcctgtg ccgaaaggca tagtcgtcgc
      961 ttcaaaagat gaggcgcggg atgcgtttcg gaagataggt ccctgcgtgc tgaaggcgca
     1021 ggtactttgg ggcagtcgag caaaggagca agccatcatc gcctgcgaca ctcagcagtc
     1081 cttagaagag gcgttcgaca gactcttcgc acgaaaggtg catgaggaaa cggttgaatg
     1141 cctgctggtc gaggaacagt tgcgcatttc gcgtgagtac tacctttcaa tcacctatcg
     1201 ggatcgcacg ccaaccgtgc ttgcttctgc acacggggga agtgaggtag tggagcggct
     1261 ggagactgat ccggatgccg tgctcgttga acccatcaac atcctgaaag ggatggacgg
     1321 agagacagca cgcacgattc tcaacaaggc cggtttcaga gacggggatg ccgtccaagc
     1381 ggcgggcatc cttgagaaat tgtatgattt cttcatacga aacgacgccc tattggcaga
     1441 gatcaatccc ttggcaaaga cctcgtcggg ggagtggtat gcagcggacg ccaaggttga
     1501 gattgatgac gacgcgctg
//
LOCUS       DAOQUC010001154         1518 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_114248_length_1518_cov_2.767601, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001154 DAOQUC010000000
VERSION     DAOQUC010001154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1518
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114248_length_1518_cov_2.767601"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(172..1395)
                     /locus_tag="PKH07_16420"
     CDS             complement(172..1395)
                     /locus_tag="PKH07_16420"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HNT36573.1"
                     /translation="MKCDEFKNLFVDYLCGELSGKDYARAEAHLQSCPECRREVAAVA
                     ETLDIVRGTPSPEPSSTVVQSLLRAAAEQPLERSWRESVPRWVLRPAFGVALAAMLIV
                     GVFITNQSFYRQPVVSEDNLGLDQMVKERSKAASTVSDVGAPAVAEQEALEQKTRARG
                     ARVQEPSGRSAGVDALPVYDVPESVGDRVTEDSEARSPEQHDPAEAQPAQAGADTWMA
                     LARASQQAPHPEAGPREISKTEAERISEKEPIVLGEASAKRAFRRADVIEQDMSSDEA
                     LSFGAPSSSAEGVALDAVSSPAVLGAPLAVSSPDIPDATTAYVQARARQIGGEHEEAV
                     HLYDIVIRQDPRFSLEGDTSHALVRRAESLVVLKRYEEAIASYRQAQEVFPEEEDALE
                     LLIGHVKDVSDEWQR"
BASE COUNT          321 a          465 c          389 g          343 t
ORIGIN      
        1 agggaccccg agaattgact atcaaggaca tgaacaccaa cattcgtttt cttggggatt
       61 accgaatgct gattattgac gcagacggct gtcggtatta tgtccccaat ttccaacagc
      121 tcgactcaac cagtctgcgc tatctctcac gatatcttct ggtgtaacca tttacctctg
      181 ccattcatcc gagacatcct tcacatgtcc gatgagcagt tccaaagcgt cctcctcttc
      241 cggaaagact tcctgggctt ggcggtaact ggcgatggct tcttcgtaac gcttcaggac
      301 gaccagactt tccgcgcgtc gcaccaaagc atgggaagtg tctccttcga gggagaatcg
      361 gggatcctgg cgaatgacaa tatcgtacag atggacagct tcctcgtgct ctcccccaat
      421 ctgtcgagcg cgagcctgca cgtaagcagt tgtggcatca ggaatgtctg gtgaagaaac
      481 tgcaagaggc gcacccagaa cggccggtga actcacagcg tcgagtgcga caccctccgc
      541 tgaggaactt ggtgccccaa aactcaaagc ctcatcagag gacatgtcct gttcgatgac
      601 gtctgccctc ctgaaagcac gcttggctga ggcttctccc aaaacgattg gctccttctc
      661 agatatcctc tcggcctcgg tcttcgaaat ctcccggggt cccgcttcgg ggtgaggagc
      721 ctgctgagat gctcttgcca aggccatcca agtgtctgcg ccggcttgag cgggttgtgc
      781 ttctgctgga tcgtgctgct caggcgatcg cgcctcagaa tcctccgtca cacgatcacc
      841 tacagattcg ggaacatcgt atacgggaag agcgtcaaca ccagccgacc gccccgatgg
      901 ttcctgaact ctggcaccac gggctcgtgt tttctgttca agagcctctt gttccgccac
      961 ggcaggggct cccacgtctg ataccgttga ggccgctttg gaacgttctt tcaccatctg
     1021 atccagccca aggttatcct cggaaacaac gggttgtcgg tagaatgact gattggtgat
     1081 gaacactccc acaattaaca tcgccgcaag tgccactccg aaggccggtc gtagaaccca
     1141 tcgaggaaca gactctctcc aagagcgttc cagcggttgc tctgcagcgg cgcgaagcag
     1201 agactgcacg accgtcgaac ttggttccgg actgggcgtt cctcggacga tgtccagggt
     1261 ctctgccacg gctgcgacct cccgccgaca ttcaggacaa ctctgcagat gcgcctccgc
     1321 acgcgcgtag tccttccccg acagctcacc gcagaggtag tcaacgaaca ggttcttgaa
     1381 ttcatcgcat ttcatgagtc cggtacctcg tgcaacaaac ccttctgcgc cagtccctct
     1441 cgaagtgact ccaaagcgta gcgcattcgg cttttggctg tgttgaccgt gcagcccaca
     1501 atctcggcga tttccgca
//
LOCUS       DAOQUC010001155         1518 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_114251_length_1518_cov_2.674641, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001155 DAOQUC010000000
VERSION     DAOQUC010001155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1518
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114251_length_1518_cov_2.674641"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(49..>1518)
                     /locus_tag="PKH07_16425"
     CDS             complement(49..>1518)
                     /locus_tag="PKH07_16425"
                     /inference="COORDINATES: protein motif:HMM:NF024924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNT36574.1"
                     /translation="LAQRVWRLILVAIPTLGLALILGRGFCGWVCPMGTLVDILDRFI
                     TPRNRYLNDLPLQTGVRPSPEVLRNPKVKYLFLTAFATAILFGVQWVHLLDPIPLLSR
                     LIATGILPQFEWLTRFVFNVFYKTAGIRVVSEPVFALLRNHVLSFKQPYYEWHWLSLL
                     IVVGIALLSRFGRRTWCRVLCPLGGLLSLCSRFAPLRVTCGDACIHCGKCARECKMGA
                     IAPESAEEPFVPQINRHECILCTTCWNICPARTLKPQFAFKTTDQNVGVDLRRRHIIG
                     SMLVGGIAAPLTALKPLRGSETTVRAIRPPGAINESEFLALCIRCGACMKVCPQNALH
                     PSLLNAGIQGLFTPELIPRLGYCHYRCNLCGVVCPTGAIQRLHQETKQRLSIGLAQFR
                     KDRCLPYAQAKNCLVCEEHCPVGEKAIQSRERAVLLPHSGETILVKEPYLVAERCIGC
                     GNCENVCPLEGLAGVTVHPRSEQILLDAVSPQQSPTSPY"
BASE COUNT          403 a          397 c          412 g          306 t
ORIGIN      
        1 acgcgtgccc aagtacgagg agcacttccg taggttcaca ggccagagtc agtaaggact
       61 ggttggggat tgctgtggag aaactgcatc cagaaggatt tgctcagacc gtggatgcac
      121 cgtgactccc gcaagacctt ccagaggaca tacattctca cagttaccgc acccaataca
      181 gcgttcggca accaagtaag gctctttgac gagtatggtt tcgccactgt gaggcagaag
      241 aaccgctctc tcgcgagact gaattgcttt ctccccaaca gggcaatgct cttcacagac
      301 gaggcagttc tttgcctgag cgtaggggag acaacggtct ttccgaaact gagcaagtcc
      361 gatgctgaga cgttgttttg tctcctggtg cagtctctga atcgctcctg tcggacagac
      421 aactccgcag agattgcatc ggtaatggca gtatccgagg cgtggaatca actcgggcgt
      481 gaagagtccc tgaatacctg cgttcaagag ggacggatgc agagcattct gtggacagac
      541 tttcatgcac gcaccgcatc ggatacaaag tgccaggaat tccgactcat tgatcgcacc
      601 tggtggacga atcgcgcgaa cggttgtctc gcttcctctc aaaggtttca gagcggtcaa
      661 gggtgctgcg atcccaccga caagcattga tccgatgata tggcgacgtc gaagatccac
      721 gccgacattc tggtcggtgg tcttgaacgc aaactgcggc ttcagcgtcc tggcagggca
      781 gatattccag caagttgtac acaggatgca ctcgtgtctg ttgatctgag ggacaaatgg
      841 ttcctcggcg ctctcaggag caatagcccc catcttgcac tcacgagcac acttgccgca
      901 atggatacac gcatcgccgc atgtgactcg aagaggtgca aatcgcgagc acaatgacaa
      961 aagtcccccc agagggcaga gcacacggca ccatgttctt ctgccgaatc gagaaagaag
     1021 agcgatgcca acaacgatga gaaggcttaa ccaatgccat tcataatagg gttgcttgaa
     1081 actcagcacg tgattacgca gcaatgcaaa gacaggttca gagaccaccc tgataccggc
     1141 ggtcttgtag aacacgttga acacaaagcg tgtcagccat tcaaactgag gaagaatgcc
     1201 tgttgcgatg agtcgggaaa gcagtggaat cggatccagc aagtgaaccc attgcactcc
     1261 aaacaggatg gcagtggcaa atgctgtcaa aaagaggtac ttgacttttg gatttctcag
     1321 gacttccggt gaagggcgaa caccagtctg gagaggaaga tcattgaggt accggttcct
     1381 gggcgtgatg aaccgatcaa gaatgtcaac caaagtaccc atcgggcaaa cccagccgca
     1441 gaaccctctc cccaggatca aggcgagacc aagagtcgga atggcaacca ggatgagtct
     1501 ccaaaccctc tgagccag
//
LOCUS       DAOQUC010001156         1516 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_114459_length_1516_cov_3.336756, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001156 DAOQUC010000000
VERSION     DAOQUC010001156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114459_length_1516_cov_3.336756"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..970)
                     /locus_tag="PKH07_16430"
     CDS             complement(<1..970)
                     /locus_tag="PKH07_16430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNT36575.1"
                     /translation="MNDNRHDRESAGSERPVVSGTPYLHEYKGHVLVVDDDEWNCELL
                     SQGLSLEGLTCAVACNGQRALEMVQQHSFDLVLLDIAMPRMSGLQVLEILRKSYTLTE
                     LPVIMVTAQEGSEFVAEALRLGANDYVTKPLDMVVLLARTQSLLTLGRLERRLREEKY
                     LVDSVINSAHEMIISVDMNRRITVFNRAAERNFGYSREEVLHQHIDMLYADPNKGMEV
                     HRQSVRENGFAGEVLNRRRNGEEFVSGLSVSVLFDQQGNPTGLMGISTDITERKKLEL
                     ERENLGRLKDEFLRIASHDLKNPVAAIYGLSRVLQGFVPPGTEMTQE"
     gene            1136..>1516
                     /locus_tag="PKH07_16435"
     CDS             1136..>1516
                     /locus_tag="PKH07_16435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011309363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine biosynthesis protein"
                     /protein_id="HNT36576.1"
                     /translation="MNRTLSEINDRIKKGTVVVATAEELIDIVREKGTKEAARTVDVV
                     TTGTFGPMCSSGIYFNTGHSKPRIKMGGGKVLLNDVPAYTGLAAVDLFLGASALPEND
                     PRNRNHPGAFRYGGAHVIEELVSGK"
BASE COUNT          372 a          403 c          390 g          351 t
ORIGIN      
        1 gctcctgtgt catctcagtc ccgggaggaa caaagccctg aagaacacgg gagagtccat
       61 agatggcagc aactggattc ttcaaatcat gactggcaat tcgcagaaac tcgtccttga
      121 gtcgtccgag attttctcgt tccagttcga gtttcttccg ctccgtgata tcggttgaga
      181 tgcccataag accagttggg ttgccctgtt ggtcgaacaa cacggagaca gagagtcccg
      241 aaacgaactc ctctccattc ctgcgccggt tcagaacctc tcccgcgaaa ccgttctcgc
      301 gcacggactg tctatggact tccatgcctt tgtttggatc ggcatagagc atgtctatgt
      361 gctggtgaag cacttcctct ctgctgtagc caaaattccg ctcggcagcc cggttgaaaa
      421 ccgtaatcct tcggttcata tccacggaga taatcatctc atgggcacta ttgatgacgc
      481 tgtcaaccag atacttctcc tcgcgaagac gcctctccaa tcggcccaag gtcagcagag
      541 actgggttcg agccaagagg acgaccatgt ccaaaggctt ggttacatag tcattggctc
      601 caagtcgaag tgcttcggca acaaactcgc tcccttcctg agcggtcacc atgatcacgg
      661 gaagttcggt cagagtatag gacttgcgga gtatctcgag cacctgcaaa ccggacatcc
      721 gaggcatcgc aatgtccagc agcaccagat caaaggagtg ttgttgtacc atctccaaag
      781 ctctctggcc attgcaggcg acggcgcagg tgagaccctc aagcgaaagc ccttgagaga
      841 gtagttcaca gttccattca tcgtcgtcaa cgaccagaac gtgccccttg tactcgtgca
      901 ggtacggcgt tccagagaca acgggtctct cagatcctgc ggactctcga tcatgacgat
      961 tgtcgttcat gccattgctc catggatggt ctgaattcgc gaatcatggc aacttctgat
     1021 gagattaccc taaaagcgct caactgcaaa atcaatctct ttttcgttcg gaagtgcaac
     1081 tattgacgaa accgcttgcg ttgtgacata aatcttcctg gatcggagga tggacatgaa
     1141 caggactctt tcggaaataa acgatcggat caagaaaggt acagtcgtag ttgcaacggc
     1201 agaagaactc attgatatcg tacgggaaaa gggcaccaaa gaagcggcca gaacggtaga
     1261 tgtcgtcacc acaggcactt tcgggcctat gtgctcgtcg ggcatctact tcaatacggg
     1321 gcactccaaa ccgcggatta aaatgggggg cgggaaagtg ctcctgaacg atgttccagc
     1381 ttacacgggt ttggctgcag tggatctttt tctgggtgct tcggcacttc cagaaaacga
     1441 cccgcgaaac aggaaccatc ctggggcgtt tcgatacggc ggcgctcatg tcatcgaaga
     1501 actggtttcg ggcaag
//
LOCUS       DAOQUC010001157         1515 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_114607_length_1515_cov_2.721918, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001157 DAOQUC010000000
VERSION     DAOQUC010001157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1515
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114607_length_1515_cov_2.721918"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..880)
                     /locus_tag="PKH07_16440"
     CDS             complement(<1..880)
                     /locus_tag="PKH07_16440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002588289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HNT36577.1"
                     /translation="MRPKTLDEFVGQEHILGQSALLRKAIEKDRIPSVILWGPPGSGK
                     TTLAAVMAHSTGARFVRFSAVTSGLKDLREVIEQAKRAREEGLKTLLFVDEIHRWNKA
                     QQDAFLPHVEDGTVTLVGATTENPSFEVISALLSRCRVFVLKALEQEHLIQILRRAIE
                     DKENGLGQFPVEISQETLEFVAVSSDGDARTALNALDLAVRIEQARHQDEGAIQLTKE
                     IFADALQKGHLLYDKAGEEHYNLISALHKSLRGSDPDAALYWIARMIEAGEHPMYIAR
                     RMVRFATEDIGNADPQA"
     gene            complement(1018..>1515)
                     /locus_tag="PKH07_16445"
     CDS             complement(1018..>1515)
                     /locus_tag="PKH07_16445"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNT36578.1"
                     /translation="IGIDQIGIGGDFAGNRPLISPTLYRDQIVPEIRRCSKVIHQANL
                     WAINASDGDLWSVIDDFLLATEVDGYLEIDMQAGMDLRRLKQAYGNRITLYGNMDCGN
                     VLSFASAEEIRRQTIACLEAGFGNGGHIFCANIAITASVPIGNYLAMVGAYREFFSMP
                     PLHFA"
BASE COUNT          326 a          436 c          406 g          347 t
ORIGIN      
        1 gggcttgagg atccgcgttg ccaatgtcct ccgtcgcgaa tcgaaccatc cggcgcgcaa
       61 tgtacatggg gtgttcgcct gcttcaatca tgcgcgcgat ccagtacagc gccgcatccg
      121 gatcactgcc ccgcagactc ttgtgcagtg ccgagatgag gttatagtgc tcctcacctg
      181 ctttgtcgta aagaagatgt cccttctgca aggcgtcggc gaatatctct tttgtcagct
      241 gaatcgcacc ttcatcctgg tgtcgcgcct gttcaattcg aacggccaga tcgagcgcat
      301 tcagcgctgt tcgggcgtct ccgtcggacg agacagcaac aaactccagc gtttcctggg
      361 aaatctccac gggaaactgt ccgagaccgt tttccttgtc ctcgatggcg cggcgcagta
      421 tctgaatgag atgctcctgc tccaaagcct tcagcacaaa gacacgacag cgggacagaa
      481 gtgccgagat cacctcaaag gatggattct ccgtcgttgc tccgaccagg gtgaccgtcc
      541 cgtcttccac atgaggtaga aatgcgtctt gttgtgcttt gttccagcga tggatctcgt
      601 ccacgaacag aagtgttttg agaccttctt cacgggcgcg cttggcctgc tcgatcactt
      661 cgcgaaggtc tttcagacct gatgtgaccg cactgaaccg aacaaatcgc gcccccgtgg
      721 aatgcgccat gactgcagcg agcgttgtct tgccagaacc cggcggtccc cagaggatca
      781 ccgaaggaat ccgatccttc tcaatggctt ttcgcaacaa ggccgactga cccagaatgt
      841 gttcctgacc gacaaactca tcgagcgtct tgggtctcat tcgatcggca agcggagcac
      901 tcgggtcgct cttccacgac tcggctccaa gaggcgattg gtctgtggtg aaaaagtctt
      961 tctgacgcat aaggcctcgc ttcaatggga cgagtatcag agaaggcgga gtcgctgtca
     1021 agcgaagtgc aagggaggca tactaaagaa ctcccgatat gctccgacca tggcgaggta
     1081 gttcccaatg ggaacgctgg cggtgattgc aatgttggcg cagaagatgt gtcccccatt
     1141 gccgaatccc gcctccaggc atgcaattgt ctgacggcga atctcctctg ccgaggcgaa
     1201 actcagcaca ttgccacagt ccatgttccc atagagggtg atcctgttgc cgtaggcctg
     1261 cttcaagcgg cggagatcca tccctgcttg catgtctatt tccaagtacc catctacctc
     1321 ggttgcgagc aggaaatcgt caatgaccga ccagaggtct ccatcactgg cattgattgc
     1381 ccaaagattc gcctgatgta tgactttgga gcaccttctg atctctggca cgatctgatc
     1441 cctgtagagg gtaggagaga tgagaggacg gttgcccgcg aaatccccac cgattccgat
     1501 ctggtctatt ccaat
//
LOCUS       DAOQUC010001158         1508 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_115477_length_1508_cov_2.930489, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001158 DAOQUC010000000
VERSION     DAOQUC010001158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1508
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115477_length_1508_cov_2.930489"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(118..603)
                     /locus_tag="PKH07_16450"
     CDS             complement(118..603)
                     /locus_tag="PKH07_16450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36579.1"
                     /translation="MPFSDPKSVLEVLSRLSELELALSDLYLECARIWPERAELWDCL
                     GREEIEHAKYLAKIEEIVREQPQSFVVHRPFALSAIETFHAAIKRRLEQVRIRGLSLR
                     DILAIARDFESSIIEMRFVDYLQFKDAQNNALAKAIEAQTQQHRKKLTDAVQAMQSSR
                     G"
     gene            complement(650..>1508)
                     /locus_tag="PKH07_16455"
     CDS             complement(650..>1508)
                     /locus_tag="PKH07_16455"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNT36580.1"
                     /translation="FKGYRVDCLTDFALDYLRTRKGDKPFFLFLSYIEPHHQNDHKHY
                     EGPNGSKERFKDFIAPGDLVGAEGDWQQEYPDYLGCINSLDMNLGRIRAELARLGQAE
                     NTLILMTSDHGSHFKTRNGEYKRACHDGCTRIPMIGCGPGFSGGKVIEELVSLIDIPP
                     TILQSAGVPIPSTMKGRPLQQLVDGACQDWPKEVFIQISESQVGRAIRTREWKYSIRA
                     VDKKGSLDPDSDVYTEDFLYNLETDPHERENLVSDDRYKSVRAELAGILKQRMVEAGE
                     KEPLILPCA"
BASE COUNT          350 a          419 c          374 g          365 t
ORIGIN      
        1 tcggatgatg atggttgccg acttacgcat gatcagtgac actccaaggt ctttcgattt
       61 gacctgtcac agcctgaaga tattcagaaa cacgaaaagt ccgaaagcaa cagacactca
      121 gccacgggaa ctctgcatgg cttgaacggc gtctgtcagt ttctttcggt gctgttgcgt
      181 ctgggcttcg attgccttcg ccagagcatt gttctgtgcg tctttgaact gaaggtaatc
      241 aacaaagcgc atttcaatta tcgaactctc gaagtcgcgg gcgatggcaa ggatatcccg
      301 cagagacagg ccgcgaatgc gtacctgctc cagacggcgc ttgatcgctg catggaaggt
      361 ttcgattgcg gacaacgcaa acggacgatg aaccacaaaa ctctgtggct gctcccggac
      421 gatctcttcg atctttgcca gatatttggc gtgctcaatt tcctctctgc cgagacaatc
      481 ccacagttcc gctctctccg gccagattct tgcacattcg agataaagat cagacagtgc
      541 caattccagt tccgatagac gactcagcac ttcaaggaca gactttgggt cgctgaaagg
      601 catgccgaaa gcctcctgtg cttcacccgt gcgagagcgg acaaaacact cacgcacaag
      661 gaagaatgag cggttccttt tcccctgcct caaccatgcg ctgtttgaga atcccagcca
      721 actccgcccg aacactcttg taccgatcgt cgctgacaag gttttccctt tcgtgaggat
      781 cggtttccag gttgtagagg aagtcctctg tatagacgtc gctgtcggga tccagactcc
      841 ctttcttgtc cacggcgcgg atcgagtatt tccactctcg tgtgcggatg gcacgaccaa
      901 cctggctctc gctgatctgg atgaacactt cctttggcca gtcttgacag gcgccgtcca
      961 caagttgctg caagggtcgc cccttcatgg tcgaaggaat gggaacaccg gcactctgaa
     1021 gaatagtcgg cggaatatca atgagactca cgagttcctc aatcactttt cccccggaga
     1081 aaccggggcc acagccgatc atgggaatcc gagtgcagcc atcgtgacag gctcgtttgt
     1141 actcaccgtt tcgagttttg aaatgagaac cgtggtcact cgtcataaga atcagtgtgt
     1201 tctctgcctg cccaagtcgt gccaactcag cacgaattcg tccgaggttc atatccagac
     1261 tgttgataca acccagatag tcaggatact cctgctgcca gtctccctca gcgcccacaa
     1321 gatcaccggg tgcaatgaaa tccttgaacc gctccttgga cccattcgga ccttcgtagt
     1381 gtttgtgatc gttctgatgg tgtggttcga tatacgagag gaataggaag aacggtttgt
     1441 ctcccttgcg ggttcgcaga tagtccagcg caaaatcagt cagacagtcc actcgatatc
     1501 ccttgaac
//
LOCUS       DAOQUC010001159         1505 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_115837_length_1505_cov_3.600690, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001159 DAOQUC010000000
VERSION     DAOQUC010001159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1505
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115837_length_1505_cov_3.600690"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..711
                     /locus_tag="PKH07_16460"
     CDS             <1..711
                     /locus_tag="PKH07_16460"
                     /inference="COORDINATES: protein motif:HMM:NF017828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HNT36581.1"
                     /translation="LMHVPTTWESPESYRLYRAEVERLGHFLVNLGGCHPSREELAAV
                     MMDYDRRRAALRNSAGCLPAGDYARMLVEFGDPARVATTVRKSGKKIPIALLGGPLRR
                     KDEAILDYLEGLGTSIVLNGTTNGEGTLPSAFDWRRVREDPFGVLTESYFHGIADAFR
                     RPNTLLYQWLQSEMRTRRVAGLVFRHYPWCDTWKAEAHRLKEWAEVPVLILEASDEPG
                     LDSSTKSRLEAFLEVLSP"
     gene            708..>1505
                     /locus_tag="PKH07_16465"
     CDS             708..>1505
                     /locus_tag="PKH07_16465"
                     /inference="COORDINATES: protein motif:HMM:NF017828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HNT36582.1"
                     /translation="MSVAPAKITLKQWEERYALLQSEGLVESSFGGSLTRHVEQGGNL
                     RLTCMEYDNSVAALELWNFLLTEEDRLHEARDQGRIIVGTMKDLGTVPVMAYSLPNVT
                     AFYPDGAWWLPCAMQLSAGLLAVADSLGIDESFCPVRAMLGAFLTEAHFPIPDLLICS
                     VGAICDDFSAIAQCLNGLGYPIFWWEIPHRRQSDPLKHGEFADAALSSRTEADQTVGL
                     PGGFIAPREQVDFVKRQLERVAEVLETVSGEQLTEERIREGIREANRI"
BASE COUNT          322 a          395 c          443 g          345 t
ORIGIN      
        1 ctgatgcatg tgcccacgac ttgggagtct ccggagtcct accgtttgta ccgtgctgag
       61 gtcgaacgtc ttgggcattt tctggtcaac cttggtggat gtcatcccag ccgggaagaa
      121 ttggcagccg ttatgatgga ctacgaccgc cgacgagcag cgttgcgcaa ttcggcaggc
      181 tgcctgccag cgggtgacta tgcgcgaatg ctcgttgagt ttggagatcc tgccagagtg
      241 gcgacaacag tccggaaatc cggcaagaaa attcctattg cccttctcgg cgggcctctg
      301 cgccgcaaag atgaggcgat tctggactat ctggaaggtc ttggcacatc gatcgtattg
      361 aacggcacga caaacgggga aggcacactt cccagtgcct ttgactggcg aagagttcga
      421 gaagatcctt tcggcgtgct cactgaatcc tatttccacg gcattgctga cgctttccga
      481 cgtcccaata ctctgctcta tcaatggctc caatcggaga tgcggacaag gcgggtggcc
      541 ggcctggtct tccgacacta cccatggtgc gacacgtgga aggcagaggc gcatcggctc
      601 aaggagtggg cggaagtgcc cgtcttgatc cttgaggcat ccgatgagcc aggattggat
      661 tcttcgacca agtcgaggtt ggaagcattc ctggaggttc tgagtccatg agtgtcgctc
      721 cagccaaaat cactctgaag caatgggaag agcgctatgc tctattgcaa tcggaaggtc
      781 ttgtcgaatc cagcttcggc ggttcgctta cgcgccacgt ggaacaggga ggcaatctcc
      841 gcctgacgtg catggagtat gacaactcag ttgccgctct cgaactctgg aactttcttc
      901 tcacagaaga agaccgtctt catgaagcaa gagatcaggg aagaatcatc gtcggcacga
      961 tgaaagacct tggaaccgtt ccggtcatgg cgtactcact tccgaatgtg accgccttct
     1021 atcctgatgg agcgtggtgg cttccctgtg caatgcagct gagcgccggt cttctggctg
     1081 ttgccgactc cttgggaatt gatgagtctt tctgccctgt tcgagccatg ctgggtgcat
     1141 ttctgaccga agcgcatttt cccataccgg acttgctgat ctgcagcgtc ggcgcgatat
     1201 gtgacgattt ctccgccatt gcacaatgtt tgaacggact gggctatccg atcttctggt
     1261 gggagattcc ccatcgccga cagagcgatc cactcaaaca tggcgaattc gcagatgcgg
     1321 ctctcagctc acggacagaa gcggatcaga cggttgggct gccaggaggt ttcatcgctc
     1381 caagagaaca ggtggatttc gtaaagcgcc agttggagcg agtcgcagaa gtgcttgaga
     1441 cggtttccgg agagcaactg accgaggagc gtattcggga agggattcgg gaagccaacc
     1501 gcatt
//
LOCUS       DAOQUC010001160         1505 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_115858_length_1505_cov_2.864138, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001160 DAOQUC010000000
VERSION     DAOQUC010001160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1505
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115858_length_1505_cov_2.864138"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..220)
                     /locus_tag="PKH07_16470"
     CDS             complement(<1..220)
                     /locus_tag="PKH07_16470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36583.1"
                     /translation="MFGTAGTNVRDYSRRTLTPFGLSTFSLVRRLLSRKVFSATAPAA
                     AFDRAGLGATARSACFSRRGHGKDHARQS"
     gene            878..>1505
                     /locus_tag="PKH07_16475"
     CDS             878..>1505
                     /locus_tag="PKH07_16475"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HNT36584.1"
                     /translation="MMRTRVGNMLVVLLLFVAVCSVDARTVTIYRIQLGAYPTKVEAE
                     AKKASAEAEGFSPVYVIEKDGEFPWKVMTGDFAEYAQAKLKCNQNQSAGLNKGEGLVT
                     QVEMKSSEAESAGLPIAEGILDQVKKQLAETHDGLRAYKRNVAQERIQVGVFDEFENA
                     VGFRCQLMAKGAAPVYVLERDGRYAVVVGDFASGEAEAERERLVSKKVI"
BASE COUNT          338 a          365 c          474 g          328 t
ORIGIN      
        1 tgctctgacg tgcatgatct tttccatgtc cgcgtcggct gaaacaagcc gagcgtgctg
       61 ttgcgccaag accggcgcgg tcgaatgcgg ctgcgggtgc tgttgcactg aaaaccttgc
      121 gagacaggag tcttcggacg aggctgaagg tggagagacc gaacggggtg agggtacggc
      181 gcgaatagtc gcgaacgttt gtccctgcgg ttccgaacac gctcccgcga taccagtgtc
      241 cggcggttca ggggtctccc agtgttgtct tgcatcgctg acgctctggc gtcctgaagc
      301 gctttgttgg tcgaattttc ggctcacatc caaaacggca gacgatgaat acgcacagga
      361 gattgaccct ccaccgcctc ggaagtctcg ttctttcaat tccatccttc cggacgcttc
      421 agaacgtctg aggaacactc cgcgccgtca gcgtgcgggc cttgaaaccg cgttccatag
      481 ttccagtttc tcatatctct acacaacttc cgatgatgaa tctccttccc aaatagacct
      541 ttcgggaatc atcccgttcg caagacggat agaagcagtg tggcatctat gccgcatgat
      601 caggaaagga gtgggggcat cttgccgcaa tctgagatcc ctgtgggacg agacgcccca
      661 cctcctgaag atttgcaccg tctccgcaaa ggtatattcc gcgaaagaac gcctcacggc
      721 gttactgcga accaaaccgc tgcacaaatg ccgtcctaag cggacgctga aaggaggtgc
      781 cgagatgact tgatgcacca gacaatgcgg aaacgatctg agtagggcag gattcatcct
      841 gcgttttggg agtaaaatag agcatggagg attgttgatg atgagaacga gagttggaaa
      901 catgttggtg gtgttgcttc tctttgtcgc cgtgtgttca gtggacgctc gaactgtgac
      961 gatctatcgg attcagttgg gtgcatatcc gacgaaagtt gaggctgagg cgaagaaagc
     1021 gtcagccgag gctgaggggt tttcgcctgt gtacgtcatc gagaaggacg gcgagtttcc
     1081 ctggaaagtg atgacggggg acttcgccga gtatgctcag gcgaagctga aatgcaacca
     1141 gaaccaatct gcgggtttga acaaaggcga aggtttggtg acacaggtgg agatgaaatc
     1201 cagcgaagcg gagtccgctg gtctgccgat agcggagggc attctggacc aagtgaagaa
     1261 gcagttggcg gaaactcacg acggtctcag ggcatacaaa cgcaacgttg cgcaggagag
     1321 gattcaggtc ggcgtcttcg acgagtttga gaacgccgtt ggatttcgct gtcagttgat
     1381 ggcgaaggga gccgcccctg tgtatgtgct ggagcgtgac gggcgatatg ccgtcgtggt
     1441 tggggacttt gcgtcgggtg aagccgaggc ggagcgtgag aggctcgtca gtaagaaggt
     1501 gatcg
//
LOCUS       DAOQUC010001161         1504 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_115963_length_1504_cov_4.010352, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001161 DAOQUC010000000
VERSION     DAOQUC010001161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115963_length_1504_cov_4.010352"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..608
                     /locus_tag="PKH07_16480"
     CDS             <1..608
                     /locus_tag="PKH07_16480"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HNT36585.1"
                     /translation="QATLRIRDESFPLHPSSPFPIPIIAMTAHAMQEDQAQCLEAGMN
                     DYLSKPITPEALTQVLERWLPGAGAALAETRKASSVNQRLGRSSDLPVFDRQGMLRRL
                     MNDEELATAVMDSFLEDIPKQIQALQDYLKTGDAPSVELQAHTIKGASANVGGEALRA
                     VAWEMEQAAKSKDLESAERFMSELEKRWLDLKKAMQESLAG"
     gene            638..1237
                     /locus_tag="PKH07_16485"
     CDS             638..1237
                     /locus_tag="PKH07_16485"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNT36586.1"
                     /translation="MRILVVDDVGYNRIIVTKALQKHGFDVVEAASGDAALQMLSRDS
                     SIGLVIADYMMPGMNGIELFKKASLIVRGKDAAAVPCPPFILLTAAQDFRVLHDAKRA
                     GFKEVLAKPLDVERLLNAIRSVLGFPEESVSSSEVSHGDTKQGEPESKASKPISPMLQ
                     QAHTQIKQAISAHDAARLAEIKKELEGAINEIEKSGLLK"
     gene            1264..>1504
                     /locus_tag="PKH07_16490"
     CDS             1264..>1504
                     /locus_tag="PKH07_16490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014777242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNT36587.1"
                     /translation="MRILVAEDDLTSRRMLVAVLQKGGYEVIETTNGAEALECLQKSD
                     APNLAILDWMMPVMDGLSVVRRIRETNLPLPPYLIL"
BASE COUNT          363 a          345 c          443 g          353 t
ORIGIN      
        1 tgcaggctac actgagaata cgagatgagt cttttcctct tcatccctca tctccttttc
       61 ctattccaat cattgcgatg accgcccacg ccatgcagga agatcaggcg cagtgtcttg
      121 aggcggggat gaacgactac ctttcgaagc cgattacccc tgaggcgttg acgcaggtct
      181 tggagcgttg gcttccagga gcgggtgcag cgcttgccga aacgcggaaa gcttcatccg
      241 tcaatcagag actcgggaga tcttctgatt tgcctgtttt tgatcggcag ggtatgctgc
      301 gtcgtttgat gaacgatgag gaacttgcga cggcggtcat ggatagtttt cttgaagaca
      361 tcccgaagca gattcaagca cttcaggact atctgaagac tggggatgca ccgtcggtag
      421 aactgcaggc gcacacgatc aagggagcgt cagccaacgt tggcggcgag gccctccgtg
      481 ctgtggcatg ggagatggag caggctgcaa aatccaaaga cctggagtcc gccgagcgat
      541 tcatgtcgga actggagaaa agatggctcg atctcaagaa ggcaatgcag gagtctttgg
      601 ccggttgaat ctattttgtt tgatggagta tcgttagatg aggattcttg tggtggacga
      661 cgtaggctat aaccgaatta tagtcaccaa ggctttgcag aagcatgggt tcgatgttgt
      721 cgaggcagca tcaggcgatg cggcgctgca gatgctttcc agagactcga gcatcggcct
      781 tgtcatcgca gactacatga tgccggggat gaatggcatc gagcttttca agaaggcgag
      841 tctgatcgtt agaggtaaag atgctgctgc agttccatgt ccgccattta ttcttttgac
      901 tgctgctcag gattttcggg ttcttcatga tgcgaaaagg gctggattca aggaagtcct
      961 ggcgaagccg ttggacgtgg agagactgct gaatgccatc cggagtgtgc ttggatttcc
     1021 ggaggaatcg gtgtcatcct ctgaggtgtc tcatggcgac accaaacagg gagaacccga
     1081 gagcaaggca tccaagccca tctctccaat gcttcagcag gcacacactc agatcaaaca
     1141 ggcgatcagc gcacatgacg cagcacgtct tgcggagatc aagaaggaac ttgaaggggc
     1201 catcaatgag atagagaaaa gtggtctgct gaaatagggg atgcattgaa aggggactga
     1261 gacatgcgaa tcctggttgc agaggatgat ctgacatcac ggagaatgct ggtggcagtg
     1321 cttcagaaag gtggctatga agtgatcgag accaccaatg gagcagaggc cttggagtgt
     1381 cttcagaaat cggatgcccc aaatctggca atccttgatt ggatgatgcc ggtgatggat
     1441 ggtctgagcg tcgttcgccg cattcgagaa accaatcttc ctcttccccc ttaccttatt
     1501 cttc
//
LOCUS       DAOQUC010001162         1504 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_115986_length_1504_cov_3.044859, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001162 DAOQUC010000000
VERSION     DAOQUC010001162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115986_length_1504_cov_3.044859"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            409..1479
                     /gene="hydE"
                     /locus_tag="PKH07_16495"
     CDS             409..1479
                     /gene="hydE"
                     /locus_tag="PKH07_16495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HNT36588.1"
                     /translation="MTDLAFLLRWLRENDEALLEQLWQQADEIRKRSVGDEVHLRGLI
                     EISSFCVRRCTYCGLRADNSALERYRMSEDEIMECVHQAIQFGYGTVVMQSGEDYGIQ
                     SEWMSGIIQRIKKETSLAVTLSLGERPEEDLCAWRRAGADRYLLRFETSDPDLYRRIH
                     PARAGQIHDRLAMLNTLRELGFEVGSGVMIGIPGQSYDILARDILTFQSMDLDMIGVG
                     PYIPHPETPLAQATWETDIPAEEQVPNTELMTYKVIALTRLVCPEANIPSTTALATLN
                     LSQGRELGLSRGANIVMPNLTPVQFRSKYEIYPAKACIRESSSDCHLCMNRRINSIGR
                     TVGKGQGGRRRKPDMENTQPIR"
BASE COUNT          384 a          397 c          379 g          344 t
ORIGIN      
        1 gaattcacaa ttgatgaagc agagccacct ctaaagcatt ggcatctcaa accaacaaca
       61 cactacaatt tattgcttaa acgttatatt gtatcgagac ggtctgtcaa gcatttttct
      121 cgatccgcct gatccattct gttttgattt caaaagccat gccttgttgt gataggatcg
      181 cgactcaggg tacttgggat tttcagccaa gtcatcctga aaaggccagc gcctcgggta
      241 cgcatactat gcccagctct gcgtggagcc cgctcgtgaa ggaagccgac tcttccaaag
      301 tctggcttcc tttgagacag agaaactttg tgccgaaaga cctgtgttgg gcattcctgc
      361 actacgatca cgattgcgct tgtgcacttg gagttgggag gaaattctat gaccgatctt
      421 gcctttctcc tgagatggct gagagagaac gatgaggctc ttctggagca gctttggcaa
      481 caagcagatg aaatccggaa gcgatccgtt ggcgacgagg tgcacctgcg aggtctgatc
      541 gagatctcca gtttctgcgt gcgccgctgc acctactgcg gtctccgagc agacaattcg
      601 gctctggagc gctatcgcat gagcgaagat gaaattatgg aatgcgtcca tcaggccatc
      661 cagttcggat acggaactgt cgtgatgcag tcgggggagg actacggaat ccaatcggaa
      721 tggatgtcag gaatcatcca acgtatcaag aaggaaacct ctcttgccgt tacactcagt
      781 ctgggtgagc gtcccgagga ggacctgtgc gcctggcgtc gagcaggtgc ggatcgatat
      841 ctgcttcgtt ttgagacctc cgatccagat ctctaccgaa ggattcatcc tgcccgcgcc
      901 ggccagatcc atgatcgcct cgctatgctg aatactctca gagaactcgg ctttgaagtc
      961 gggagcggtg tcatgattgg tattccagga cagagctatg acattcttgc gcgtgacatc
     1021 ttgaccttcc agagcatgga tctggacatg attggagtgg gtccctacat cccccatcct
     1081 gagaccccac tcgctcaggc gacttgggag acagacatac cagccgaaga acaggttcca
     1141 aacacagaac tcatgaccta caaagtcata gcgttgaccc gtttggtctg ccctgaagcc
     1201 aacatcccaa gcacaactgc ccttgcaacg ctcaacctct ctcaaggcag agaactggga
     1261 ctcagccgcg gagcgaacat tgtcatgcca aatctgacgc ctgttcaatt ccgttccaaa
     1321 tacgagatct atccagcaaa ggcgtgtatc cgtgaatcct cttcagactg tcacctctgc
     1381 atgaatcgga gaatcaactc aatcggcaga accgttggaa agggacaagg tggaagaaga
     1441 aggaaaccag acatggaaaa cacacaacca atcaggtgag attcatctca accaaccgct
     1501 tagg
//
LOCUS       DAOQUC010001163         1504 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_116007_length_1504_cov_2.806763, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001163 DAOQUC010000000
VERSION     DAOQUC010001163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116007_length_1504_cov_2.806763"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..635)
                     /locus_tag="PKH07_16500"
     CDS             complement(<1..635)
                     /locus_tag="PKH07_16500"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNT36589.1"
                     /translation="MNSHAVHAKSQRILILSGTGYPLRSGMVKWIEQLGAALANAGCE
                     VRQIVASEMPTIGWTGCYQIQSLGDSRRRKQIGNHYLQGFRRFRMVHRIVAGMLTTGW
                     NPNLVISDSTPSMLLLAHRTALQGNVPRLVIAGGDFFREEPRWLLRNMVRHLYKRATG
                     IFVDGADLQAVLAKEGINQQSLHVIPHGVDTDVLTPGVSSELFFHFLGEKNL"
     gene            724..1497
                     /locus_tag="PKH07_16505"
     CDS             724..1497
                     /locus_tag="PKH07_16505"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNT36590.1"
                     /translation="MMSKEENGRTLRSIIHFNDRLGCVEAARSLDYFRCIEYLLVLDL
                     LDLKPDLQLLDLGVGKGPFSSYLASEKGIQVLALDIAPKAIGWQAQKAGRLRLKNSQF
                     VPIVGDSRSLSFADESFDRVLNLGSIEHVPGDGDRLTAKEMGRVLRTGGRAIFSIPYG
                     RRYTESDTSPHVRYFERRYDEKALQERIIQPSGLKEISRFYFGEPSLKWSRFWYSVPF
                     VLKLPFRRATRLMASRWLSIIPAEDKDSACGICFALAKE"
BASE COUNT          353 a          419 c          379 g          353 t
ORIGIN      
        1 agattcttct cgcccaggaa atgaaagaag agttctgaag agacacctgg cgtgaggacg
       61 tctgtatcaa caccatgcgg aataacgtga aggctctgct gattgatccc ttctttggca
      121 aggactgcct gaagatccgc cccgtcaaca aagatcccag tcgccctctt atagagatgt
      181 ctcaccatgt tcctaagcag ccatcgtggc tcttcccgaa agaagtcccc acctgcaatg
      241 acgagacgcg gtacattccc ttgtagcgca gtccgatgtg ccaagagtaa catgcttggg
      301 gtggaatcgc tgatgacaag attcggattc caacccgtcg tcagcatccc cgctacaatt
      361 cgatgtacca ttcggaacct acgaaacccc tgcaaataat gatttccgat ctgtttccga
      421 cgccgcgagt ctcccaacga ctgaatctga tagcaccccg tccaaccaat ggttggcatt
      481 tcagaagcaa caatctgtcg aacttcacat ccagcatttg ccagtgcggc ccccagttgc
      541 tcaatccact taaccatccc acttcggagc ggataacccg ttccactcag gatgaggatg
      601 cgctgtgatt tcgcgtggac tgcgtgagag ttcatccgag aatccgcccg cctaccaaag
      661 acgagagtgc ctccatcttt tgagtttcat actcacctaa cgtctgttat actccgaacc
      721 aatatgatga gtaaagaaga gaacgggaga acgctcagga gcatcataca cttcaacgac
      781 cgactgggat gcgttgaagc agcgcgatct ctggattatt tcaggtgcat tgaatacctt
      841 ctggtgcttg atctgctcga tctgaagccc gatctccagt tgctggattt gggcgttggc
      901 aaggggcctt tttcatccta cctcgcctcg gaaaagggaa tccaagtctt ggctctcgat
      961 atcgctccca aggcgatagg ctggcaagct cagaaggctg gacgactcag attgaagaac
     1021 tcccagttcg tgcccattgt tggagacagc cgttcccttt ccttcgccga tgaatccttt
     1081 gatcgagttc tgaatctggg atcgattgag catgtccccg gggatggtga cagactgact
     1141 gccaaggaga tgggcagagt tctccgcacg ggcggtcgtg ccattttctc catcccctac
     1201 ggcagacggt acacagaatc cgatacgagt ccccatgtca ggtacttcga aagacgatac
     1261 gacgagaagg cacttcagga gaggatcatc cagccctctg gtctgaagga gatatcccgg
     1321 ttctatttcg gtgaaccctc gctgaagtgg agcaggttct ggtactcggt tccctttgtg
     1381 ctcaagttgc catttcgccg agcgactcgc ttgatggcgt cacgctggct cagcatcatc
     1441 cccgctgagg acaaggatag cgcttgcggc atctgcttcg ccctggccaa ggagtgaagg
     1501 atgc
//
LOCUS       DAOQUC010001164         1504 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_116072_length_1504_cov_1.907522, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001164 DAOQUC010000000
VERSION     DAOQUC010001164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116072_length_1504_cov_1.907522"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            143..967
                     /locus_tag="PKH07_16510"
     CDS             143..967
                     /locus_tag="PKH07_16510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011404186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HNT36591.1"
                     /translation="MQGTLPELFLHLDPIAQAFWATLFTWFVTALGASPVLFTRRLNQ
                     KLLDGMLGLAAGVMIAASFWSLLNPAIEMAGGDWVPATVGFLLGAGFLYVVDKILPHL
                     HPGLEMSEAEGIKTSWQRSVLLVLAITLHNIPEGLAVGVAFGAVAHAADAIAARQMML
                     GGVALAIGIGLQNFPEGLAVSMPLRREGMRRGKAFFYGQISGAVEPIAGVLGAALVLA
                     MQPILPYALAFAAGAMIFVVVEELIPESQRNGFSDFATAGAIVGFAIMMTLDVALG"
     gene            1090..>1504
                     /locus_tag="PKH07_16515"
     CDS             1090..>1504
                     /locus_tag="PKH07_16515"
                     /inference="COORDINATES: protein motif:HMM:NF013183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HNT36592.1"
                     /translation="MTVQPSSRTAELVMVGSELLRGVYADTNGCFLARSFAAEGIAVT
                     RTTVVRDVEDEIAEVISRALSRVSLVVVCGGLGPTIDDLTREAIVQATGIPLALHQEI
                     VDAIRQRFASRGLTVSENNLRQAYVPECGGFFPNPN"
BASE COUNT          303 a          397 c          458 g          346 t
ORIGIN      
        1 tcaggaaggc gatgttggga gaattctcga acgtcaaggc aactggatcc ggatccggac
       61 tttggacaac aaggtcggct gggtcagcgc caactacgtg accgtggttg gccaatagtt
      121 cggcggactg ccaagagaga agatgcaggg aacgcttccg gaattgtttc tgcacctcga
      181 tccgatcgcg caggcgttct gggcgacctt attcacttgg tttgtcactg cactgggtgc
      241 ctcacccgtg cttttcaccc gccgtctgaa ccaaaaactc ctcgacggaa tgctcggctt
      301 ggctgccggt gtcatgatcg ccgccagctt ctggtcgcta ctgaatcctg ccatcgaaat
      361 ggcggggggt gactgggtac ccgccacagt gggttttctt ctcggcgctg gatttcttta
      421 tgttgtggac aagatcctgc cacatctgca tccgggactg gagatgtcgg aagcggaggg
      481 aatcaagacg tcatggcaga gaagcgtcct gctggtgttg gcgatcaccc tgcacaacat
      541 tcctgaagga cttgcggtgg gggtggcctt tggagccgta gcacacgcgg cggacgcgat
      601 tgccgcgcga cagatgatgc tcggcggtgt tgcccttgcc attggcatcg gactgcagaa
      661 cttccccgaa ggtcttgccg tctcaatgcc tcttcgacgt gagggaatga ggcgaggcaa
      721 ggccttcttc tacggacaga tatccggcgc agtggaacca atcgccggcg ttcttggtgc
      781 tgcgcttgtg cttgccatgc agccgatcct tccctacgct ttggcctttg cggcaggagc
      841 catgatcttc gtcgttgtgg aggaattgat tccggaatcc caacgaaacg ggttcagcga
      901 tttcgctacg gcgggtgcca tcgtgggatt cgccatcatg atgactctgg acgtggcgct
      961 ggggtgaacg agatcggctc gtcttgacgg gatcacatga cccgggctat tcctctcctg
     1021 taaggcacac gttcgtgata taagaatgcc tctcctgaag tcaagtttgc cgggagaaag
     1081 acaagacgaa tgacagtaca accctcttct cgaactgcag aactagtcat ggtcgggagt
     1141 gaactcctgc ggggtgtcta tgccgacaca aacggctgct tcctggcacg ctccttcgca
     1201 gctgagggga tcgccgtcac gagaacaacc gtggtcagag atgtcgaaga cgaaatcgct
     1261 gaagtcatta gtcgtgcctt gagtcgggtt tccttggtcg ttgtttgcgg aggtctgggg
     1321 cccacgattg atgatctgac ccgagaggca atcgtacagg ccacagggat tcctctggct
     1381 ctgcaccagg agattgtgga cgcgatccgg cagcgatttg ccagcagagg attgactgtc
     1441 tcagagaata acctcaggca ggcctatgtt ccagaatgcg ggggattttt tccaaacccg
     1501 aatg
//
LOCUS       DAOQUC010001165         1501 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_116374_length_1501_cov_2.983402, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001165 DAOQUC010000000
VERSION     DAOQUC010001165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116374_length_1501_cov_2.983402"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..928
                     /locus_tag="PKH07_16520"
     CDS             <1..928
                     /locus_tag="PKH07_16520"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNT36593.1"
                     /translation="PEMKRSLTELLQKHAFDTVQVDYYFMMQNVPESLSQPVLLQNIV
                     LDTGLYADCAVRARNPLVRLAYRRLARQCGAWELRGWKRADLNVAITPELSQQMRQLG
                     FRDQIEVQLPGLDMEYYQPGDAFEQGKEDLVFVGAMRYPPNVDAMLYFCREVLPLIRE
                     RRPNIRLWIVGQHPVPEIVALGKEPNVNVTGFVDDVRPYWNRGTVAVLPLRIGGGVRL
                     KMLEALAMGKAIVTTESGAEGSSAEPGKEFLQASSSEEMRDQILRLLDDGELRRTLQR
                     NARQAAVNRYSWEICGQRFEKMHEWLLTERTC"
     gene            948..>1276
                     /locus_tag="PKH07_16525"
     CDS             948..>1276
                     /locus_tag="PKH07_16525"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNT36594.1"
                     /translation="MNTESRDQPTRPDLSIVVVNYCRWDLTRNCLASIAEACKGITHE
                     VFLIDNASKEDHCDAIAKEFPNVTVRRMQENLGFAKGNNVGLRLSKGRYAMLLNNDTV
                     CFPESLT"
     assembly_gap    1277..1286
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1312..>1501)
                     /locus_tag="PKH07_16530"
     CDS             complement(1312..>1501)
                     /locus_tag="PKH07_16530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36595.1"
                     /translation="LSVVRSQSVKRYPVENRFLGKILEKRDFDENNAVANDGKLLPVC
                     CGAPFTSRSLQPPTPAFA"
BASE COUNT          371 a          346 c          430 g          344 t
ORIGIN      
        1 tccggagatg aagcgttctc tgacggaact gcttcaaaaa catgcgttcg acacagttca
       61 agtggattac tacttcatga tgcagaatgt gccggaatca ctcagtcagc ctgtcttgct
      121 tcagaacatt gttctggata caggcttgta tgccgactgc gccgtgcggg cgagaaaccc
      181 gttggttcgg ttggcatatc ggagacttgc aaggcaatgc ggtgcgtggg agttgagggg
      241 atggaaaagg gctgacctca acgtggccat caccccggaa ctttcccaac agatgaggca
      301 actcggtttc agagatcaga tcgaggttca gctgcctggg ctggatatgg aatactacca
      361 accgggtgat gcgtttgaac aaggcaagga agatttggtt tttgtcggtg cgatgaggta
      421 tcctcccaac gttgacgcca tgctctattt ttgccgtgaa gtactgcctc tgatccgtga
      481 gagacgtccg aacattcggc tttggatcgt gggacagcat ccagttccag agattgtggc
      541 tctcgggaaa gagccaaatg tcaatgtgac tggattcgtg gatgatgtgc gaccttattg
      601 gaataggggg actgtcgccg tgcttccgct gagaattggt ggaggagttc ggctcaaaat
      661 gctggaggcc ctggcgatgg ggaaagccat cgtgaccaca gagtctggcg cggaaggatc
      721 gagtgccgaa ccaggcaagg agtttcttca agccagttcc tctgaggaga tgcgcgatca
      781 gatactgaga ctgctcgacg acggagaact gcgaagaaca ctgcaaagaa acgctcggca
      841 agcggccgtg aatcgctaca gctgggagat ctgcggacag agatttgaga aaatgcacga
      901 atggttgctt actgaaagga catgttgacg tctatcttgg tgcggagatg aacacagaga
      961 gcagagacca acccacgcga cccgatttgt ccatcgtcgt cgtcaattat tgccgatggg
     1021 atctgactcg caactgtctg gcttcgattg ccgaagcatg caaagggatc acccacgaag
     1081 tgtttcttat tgataatgct tccaaagaag atcattgcga tgccattgcg aaggagtttc
     1141 cgaacgtaac ggttcgtcga atgcaagaga atcttggctt tgccaagggg aacaacgttg
     1201 gtctgaggct ctcaaagggg cgctatgcaa tgctcctgaa caatgacaca gtctgctttc
     1261 cagaaagcct gaccaannnn nnnnnnccaa agccgttcgc ttcatggaca gtcacgcgaa
     1321 agccggagtg gggggctgca aactcctgga tgtgaaggga gctccgcaac agacaggcag
     1381 aagttttcca tcgtttgcga cggcgttgtt ctcatcgaag tctctctttt caagaatttt
     1441 ccccaagaac cggttctcca caggatacct cttgactgat tgggatcgaa ctactgacag
     1501 a
//
LOCUS       DAOQUC010001166         1499 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_116657_length_1499_cov_3.365651, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001166 DAOQUC010000000
VERSION     DAOQUC010001166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1499
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116657_length_1499_cov_3.365651"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..725)
                     /locus_tag="PKH07_16535"
     CDS             complement(<1..725)
                     /locus_tag="PKH07_16535"
                     /inference="COORDINATES: protein motif:HMM:NF025104.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36596.1"
                     /translation="MPPKGPAHKFIAVDLFVMLAAVALAVVSWLGFPPNPPNVVLYKH
                     TLLVIVLIRLFLHYTYGLYDFRANNNAVDVLYNTFCAVSLGSVIELCVLQFAYIYYAN
                     GLDERSLAVGQSPVQLSRIVVVLTWVYSLIGFSTWRLLYLRRRRRWAYDLTTVLIVGA
                     GEFGETVRQEIEQYSRMGHRVVGFIDDEIDETQVTSSGQILGKLEDIRQIVQEHKVDE
                     IIVTSNRASRGELLAILSQCEQTR"
     gene            complement(729..>1499)
                     /locus_tag="PKH07_16540"
     CDS             complement(729..>1499)
                     /locus_tag="PKH07_16540"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNT36597.1"
                     /translation="RLLARCRTDRIICVSESDAASVKSCLRLTSSSDILSWVPNGIEY
                     ERFASAERDDDYRRRLGVEDGEIALGTVGRLRPQKALGTLVLLGRLLKEARWKFKILI
                     VGEGFLEQELRLRIRDCGLEEEVRLLGYREDIPRFLQALDVFLLVSLWEGFPIAVLEA
                     MAAGLPIVATRVPGTQELLEPVYGNRLAAVRDPDALMRLLGDWRTNREQWQTLAEEGR
                     MFVRANYTVEKMVASTEQIYQHLLEGTANPMPSRGHRG"
BASE COUNT          383 a          435 c          366 g          315 t
ORIGIN      
        1 cgggtctgtt cacactgcga gagaatcgca agcagctccc cacgactcgc tctgtttgac
       61 gtgacaatga tctcatcaac cttgtgttcc tggacgatct gtcgaatatc ttcgagtttt
      121 cccagaatct gacccgacga ggtaacctgg gtttcatcaa tctcatcatc aatgaagccg
      181 acaacacgat gacccatccg tgaatactgc tcaatttcct gacgaacagt ctctccaaac
      241 tctcctgcac cgacgatgag cactgtggtc aggtcatagg cccatcgtct tcgccgccgc
      301 aagtacagca gacgccaggt actgaatccg attagcgagt agacccacgt caaaacaaca
      361 acgatgcgag acagctgcac cgggctctgt ccgacggcca aagacctctc gtcgagtccg
      421 ttggcatagt agatataagc aaactgaagc acacacaact cgatgactga gccgagcgag
      481 acagcgcaga acgtgttgta gagaacatct acggcattgt tgtttgcgcg gaaatcataa
      541 aggccgtagg tgtagtgcag gaaaagacga atcaggacga tcaccagcaa agtgtgtttg
      601 taaagaacaa catttggtgg attgggcggg aaaccgagcc aggaaaccac ggccaaggcg
      661 accgctgcca gcatgacaaa gagatcgacc gcaataaact tatgtgccgg ccccttggga
      721 ggcatcagtt accctctatg gcctcgggaa ggcatcggat ttgctgtgcc ctccagtagg
      781 tgttgataaa tctgctccgt tgatgcaacc atcttctcaa cggtgtagtt ggctcgaaca
      841 aacattctgc cctcttcggc aagagtctgc cattgttctc tgttcgttct ccagtcgcca
      901 aggagcctca tcagcgcgtc agggtctcga accgccgcaa gcctgttccc gtagaccggc
      961 tccaggagtt cctgggttcc tggaactcgc gttgcaacga ttggcagtcc tgcagccata
     1021 gcctccagaa ccgcgattgg gaaaccttcc cacaaggaga ccagtaggaa aacgtccaac
     1081 gcttggagaa acctagggat atcctctcga tatcccagca gacgaacttc ctcctccagg
     1141 ccacagtccc gaatccgaag ccggagttcc tgctcgagaa aaccttcacc cacgatcaga
     1201 atcttgaatt tccatcgggc ttccttcaag agacgaccga gcagcaccag agtgcccagt
     1261 gccttctgtg ggcgcaatct tccgactgtt ccaagagcga tttcaccatc ctcaacaccg
     1321 agacgccgac ggtaatcgtc atcacgctca gcagacgcaa accgctcata ttcgataccg
     1381 tttggcaccc atgagagtat atcactcgac gatgtcaacc tcaaacagct ttttaccgat
     1441 gcggcgtccg actcggaaac gcagatgatg cgatctgtac gacatcgggc aaggagtcg
//
LOCUS       DAOQUC010001167         1499 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_116663_length_1499_cov_3.109418, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001167 DAOQUC010000000
VERSION     DAOQUC010001167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1499
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116663_length_1499_cov_3.109418"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(225..1196)
                     /locus_tag="PKH07_16545"
     CDS             complement(225..1196)
                     /locus_tag="PKH07_16545"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNT36598.1"
                     /translation="MLSRERVIAVINHQKPDRIPVYGWVSANLTEPITKAFGSVEAFE
                     DRYEFDFAHLFGGPSCFSNDELKQARQARGGSLTPKDILQIPMCDPNKSEDYQNIIDA
                     IRHHKHERGRFVYVQTPGIFEALNGGFGIEEHLRHLAEFEDDLHLVYKRQAEWNRDFA
                     NNCIDLGVDMVHVSDDWGAQHGLMFSPKTWWSLIYPYHKITCDLVKSRKTFVSVHSDG
                     NVNAVVDGIIKLGYDVVHPWQESAGMSLQQYKENFMSHFTVMGGLDVQTTIGFGKLDF
                     LRSEIQRVMGMFAEGGLLLCTTHFVQPHCTIEELTLAFDLIQELRTI"
     gene            complement(1230..>1499)
                     /locus_tag="PKH07_16550"
     CDS             complement(1230..>1499)
                     /locus_tag="PKH07_16550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HNT36599.1"
                     /translation="LARPGLVTAGIFVFIGLWNEYPLALVLLTSETSQTLPVGIANLT
                     MTQKYQADWGALFAGLTIAVIPTVLLYSVFQKQIQAGLTAGAIKG"
BASE COUNT          359 a          372 c          411 g          357 t
ORIGIN      
        1 cactgacggt gagtactcgg actcagagac ggtcacgata gcgattcaga gcagcacaga
       61 cattccgaaa gtgctctggg agtccgccaa gtagaccgca gcaggtggat gctgcgttga
      121 tcgtagtgta cctttgcgaa cccgtgaggc gagagcgctt cacgggttcg tcgttgagaa
      181 gaagagggat acctgtaaga cagcccacat cagagagcgc cagttcagat cgttctcagt
      241 tcctgaatca ggtcaaacgc aagagtgagt tcctcaatcg tgcagtgtgg ctgcacgaaa
      301 tgtgtagtgc agagaagaag tcccccttcg gcgaacatgc ccattacgcg ctggatttca
      361 gagcgcagaa aatccagttt gccgaagccg atggttgtct gtacatcgag tccgcccatg
      421 acggtgaaat ggctcatgaa gttctctttg tattgttgaa ggctcatacc ggcggattcc
      481 tgccacggat ggacgacatc gtagccgagt ttgatgattc catccacgac ggcgtttaca
      541 ttgccgtcgc tgtggacact cacgaaggtc ttgcgagact taaccaaatc acaggtgatt
      601 ttatgatagg gatagatcag actccaccag gttttgggac tgaacatgag tccatgctgc
      661 gctccccagt cgtcggacac atgtaccatg tccacgccta gatcaatgca gttgttggcg
      721 aaatctcggt tccattctgc ctggcgttta tacaccaggt gcaggtcgtc ttcgaactca
      781 gcgagatggc gcaggtgttc ttcgatgccg aacccaccgt tgagtgcctc aaaaatccct
      841 ggagtctgaa catagacgaa acgcccgcgt tcgtgcttgt ggtgtcgaat ggcatcaata
      901 atgttctgat agtcttcgga tttgttggga tcgcacatcg gaatctggag gatatctttg
      961 ggtgtaagtg agcctccgcg agcctgtctg gcttgcttca gttcatcgtt tgagaaacag
     1021 gacggtccgc caaaaagatg cgcgaaatca aattcatatc tgtcctcgaa cgcctcaaca
     1081 gatccgaatg ctttggtaat gggctctgta aggtttgcgg acacccagcc atagaccgga
     1141 atccgatcgg gtttctgatg attgatcacg gcaatgacac gttctctcga cagcatactg
     1201 ctttccctcc acaagtagaa atccggattt catcctttga tggctccggc cgtgaggcct
     1261 gcctggattt gtttctgaaa aactgagtag aggagaactg ttgggatgac cgcgatggtc
     1321 aagccggcaa acagggctcc ccagtccgcc tgatatttct gagtcatggt cagatttgcg
     1381 atgccgacag gaagagtctg ggaagtctcg gaggtcagca gcaccaaggc aagagggtac
     1441 tcgttccaca gtccgatgaa gacaaagatt cctgcagtaa cgaggcctgg ccttgccaa
//
LOCUS       DAOQUC010001168         1499 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_116725_length_1499_cov_2.167590, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001168 DAOQUC010000000
VERSION     DAOQUC010001168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1499
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116725_length_1499_cov_2.167590"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /gene="hisG"
                     /locus_tag="PKH07_16555"
     CDS             complement(<1..358)
                     /gene="hisG"
                     /locus_tag="PKH07_16555"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HNT36600.1"
                     /translation="MSRQVLLGVPKGSLQESTLALFAKAGFSFYGSSRSLWLSSNDQE
                     IKPVLLRPQEIPIYVANGSLDAGLSGWDWITETKVTDKVRILADLCYSKRTFRPVRWV
                     LAVAEDSPYQTVDDLRT"
     gene            complement(583..1158)
                     /locus_tag="PKH07_16560"
     CDS             complement(583..1158)
                     /locus_tag="PKH07_16560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36601.1"
                     /translation="MRKVVRVTWPGIFLLLFGCIPSLHPLYTEEDLIFDPALVGIWAN
                     EDSGESWTFTKTSEKEYKTVYIDNSKAKGEFSGHLVKSGDCIFLDLYPSKPELDENDF
                     YKIHLIPVHTFLLVRQVEPKLQMAILKPDALKKVLTANPDAIKHEKVEDGVLLTAQPK
                     ELQAFLVNLAKTAENWEDMDFLPRKVLETTE"
BASE COUNT          381 a          401 c          359 g          358 t
ORIGIN      
        1 tggttcgcag atcatcaact gtctgataag gcgagtcttc cgccacggca agcacccatc
       61 gcaccggacg aaatgtccgc ttcgaatagc acagatcggc caggattcgc accttgtccg
      121 tcaccttcgt ctcggtgatc cagtcccaac ccgaaagtcc cgcgtcaaga cttccattgg
      181 cgacatagat gggaatctcc tgaggtcgaa gcaacacagg ctttatctcc tgatcgttcg
      241 agctcagcca cagggatcgg ctcgacccgt agaaggaaaa acccgctttg gcgaaaaggg
      301 caagtgttga ttcctgaagg cttcccttcg ggacgcccag gagcacttgt cggctcatgt
      361 cagacgctcc tccgcttttg aaaatgaaga cagaacaccc cgcttggcag cagagcactc
      421 ctgtcggcaa accttattat caaataagaa tgccaaaggc ctgtcaagcc tttttgccga
      481 gaaatgggaa aacagaggcc aaaacctggg ctgaaacagg gtccgattac atctggcatc
      541 gtcgagccct gtccgcgagg tgaacaggct cggacgccct tatcattcgg ttgtctctag
      601 cacctttcgt ggaaggaaat ccatgtcttc ccaattctcc gcagtctttg caaggttcac
      661 cagaaaagcc tgcagttcct tgggctgcgc agtcagaaga acaccatctt ccaccttctc
      721 atgcttgatg gcatcgggat tggctgtcaa aactttcttg agtgcatctg gcttaaggat
      781 cgccatctga agttttggct caacttggcg cactagcaag aacgtgtgca ctgggatgag
      841 atggattttg tagaagtcgt tctcgtctag ttccggtttg ctcgggtaaa ggtccagaaa
      901 aatgcagtct cctgatttca ccagatgccc agagaactca cctttcgctt tggaattgtc
      961 aatgtaaacc gtcttgtact ccttctcaga ggtcttcgtg aaagtccaac tttctccact
     1021 gtcttcgttc gcccatatac cgaccaaagc ggggtcgaag attagatcct cttccgtgta
     1081 gaggggatgc aaagacggaa tgcacccaaa cagcaggaga aagattccag gccaggtgac
     1141 tcgtactact tttcgcatcg caatatcctt tctcagaaca gcagatatgc ccaaaactct
     1201 cgctatcaag aaagcattat agcactctct ccagcatgca acaagatcga gcgggaatac
     1261 ggatttagct ggacgtttca gccgaagaat gcagaatatc aggaaggttc cattgaccga
     1321 aggttcaaac caaactgacg ggatatatga cctatatcct ctcttggata ggaagacagg
     1381 gaacacgatg aaaccaggaa ttctgttggt agccacagta tgtcttgccg cctctccatt
     1441 cctgttcgtt tcaccaagcc acggagcttg agttcccgga agatcagtcc ggcttcgat
//
LOCUS       DAOQUC010001169         1497 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_116895_length_1497_cov_4.148405, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001169 DAOQUC010000000
VERSION     DAOQUC010001169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1497
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116895_length_1497_cov_4.148405"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /locus_tag="PKH07_16565"
     CDS             complement(<1..358)
                     /locus_tag="PKH07_16565"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNT36602.1"
                     /translation="MRRRVWILILILLVACGFRLYRVTNPLLDSHHWQQGTTASIAWH
                     YYFTDGNFFHALESGWGAHPKLFLNELPLFPYLVSMIYRIIGFQEIAGRLLSIFFGLL
                     ALVLLYLIASKYLGEAQ"
     gene            complement(593..1033)
                     /locus_tag="PKH07_16570"
     CDS             complement(593..1033)
                     /locus_tag="PKH07_16570"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HNT36603.1"
                     /translation="MEMPAKERPFDPKAQDSVATTTLGPDTEFTGNMKFGKTLKIEGK
                     FEGELTSEGTLIVASTGKVKATQIAVGSAIIEGKVEGPVVAKELIELRSRAEILGDIK
                     SKRLKIDDGVVLVGKCEVRPHGSEVSEPARPAAAPAPQQKPANK"
     gene            complement(1268..>1497)
                     /locus_tag="PKH07_16575"
     CDS             complement(1268..>1497)
                     /locus_tag="PKH07_16575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36604.1"
                     /translation="NLSAERGDLLDQYTHFAISTGLDADLGVVFGCNIRSGFAVAKAM
                     GLVRSHQNVSRRNKFIRMLLESSNHAFAHGT"
BASE COUNT          344 a          432 c          365 g          356 t
ORIGIN      
        1 cttgtgcctc accgagatac ttgctggcga tcaggtaaag aaggacaagg gcaagcaggc
       61 caaagaagat tgaaagaagc ctgcccgcga tctcctgaaa accgataatt cggtagatca
      121 tactgacaag gtaaggaaac agtgggagtt cattcagaaa cagtttcgga tgggcacccc
      181 atccgctttc aagggcgtga aagaaattgc cgtctgtgaa gtagtaatgc caggcaatgg
      241 acgctgttgt tccctgttgc cagtgatgag aatccagcag aggattggtg acccgataca
      301 gtcgaaaacc acaggcgacc agcaggatca agatcagaat ccaaacacgt cgcctcatct
      361 tcgtctcctg aaccgcacac tccagatcta cgtcatccac agattggcgg tctcgtgtgg
      421 gagttgtccc acgattccga acgagtcagt ttccgttgcg ggacgcctct catgattctt
      481 gcccttgcca gacgcagcgg gaaaatccgg cgtacgagtt caaccttcgg ggctgtctta
      541 tgaaaacacc cgtgaaggtt cctaatgcag gaaatggctg ccctggaaat tcctacttgt
      601 tggcaggctt ctgctgcggt gccggagctg cggcgggccg agccggttca gaaacttctg
      661 atccgtgtgg ccgtacttcg cacttgccca ccaaaaccac gccatcatca attttcagcc
      721 tcttggactt gatatcacca agaatctcgg cacggctcct gagctcaatc aactccttgg
      781 caaccacggg accttcgacc tttccctcaa tgatcgcgct gccaacggcg atctgggtcg
      841 ccttgacttt tccggtagac gccactatca gcgttccctc ggaagtgagt tctccctcga
      901 atttcccctc gatcttgagg gtcttcccga atttcatgtt cccagtgaat tccgtatccg
      961 gccccagtgt cgtcgttgcg acagaatcct gtgccttggg atcgaacggt cgttcttttg
     1021 ccggcatttc cataataatc cctgtctcct ttttctcaga gttctctaat cgcactcaga
     1081 cttctcaagg caagcctgag tttgttactc atccacctaa atatggcacg tgctgtccag
     1141 tcttccaagc gactcacaac ccgttcaggc cgacagcgtt gcccccgtgc cagagtccct
     1201 tataggaaac ttcgctcgcc cttgtcaact ggaatgcaga gcatgccatc gtgaatacca
     1261 agcctgctca tgttccgtgc gcaaaagcgt ggttgctcga ttccagcaac atacgaatga
     1321 acttgttcct tcgggaaaca ttctggtgtg aacgcaccag ccccatagcc tttgccaccg
     1381 cgaatccgga acggatattg cacccgaata cgacacccag atcggcgtcc aagcctgtgc
     1441 tgatggcaaa gtgcgtgtac tgatcgagca aatcacctct ttcggcgctg aggttgg
//
LOCUS       DAOQUC010001170         1497 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_116957_length_1497_cov_2.444521, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001170 DAOQUC010000000
VERSION     DAOQUC010001170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1497
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116957_length_1497_cov_2.444521"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1128
                     /locus_tag="PKH07_16580"
     CDS             <1..1128
                     /locus_tag="PKH07_16580"
                     /inference="COORDINATES: protein motif:HMM:NF012308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HNT36605.1"
                     /translation="ATVTGNNTFALDLYSILRKEEGNLFFSPYSLSSALAMTYAGARG
                     DTAAEIAKVLHFTQGSDGMHPALADLNKVFDTIQKAGHVRLHIANSLWPQKDHPFQPD
                     YLTLLKEHYGASITPVDYVTATEQAREKINKWVEVKTKEKITNLIPPGVLNHLTRLVL
                     VNAIYFKGDWKSQFDPINTRESEFTVSKHDEVKVSMMHQKGRFGYGETSGAQLLELPY
                     LGSDLSMVVILPQTVDHLPEIERQLTEANLHLWLSSLSKQEVLVWLPKFKVTWGVFSL
                     KEPLQYLGIRKAFDPLGADFSGMDGTRELFITQVLHKAFVEVNEEGTEAAAATAAVVG
                     RTMALHRTQTFKADHPFLFLIRDNVTGSILFLGRVLNPSKN"
     assembly_gap    1190..1199
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1331..>1497)
                     /locus_tag="PKH07_16585"
     CDS             complement(1331..>1497)
                     /locus_tag="PKH07_16585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013896394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HNT36606.1"
                     /translation="RLTTHNQGGSPHTSKYAPWRIVTAIRFDDDQRASEFERYLKSGS
                     GRAFANKHLW"
BASE COUNT          380 a          377 c          399 g          331 t
ORIGIN      
        1 gccaccgtta cgggcaacaa cacctttgct cttgacctgt actcaatcct caggaaggag
       61 gaaggaaacc ttttcttctc gccatacagt ctttcgtcgg cactagccat gacatacgca
      121 ggcgcgagag gcgacacagc tgcggagatc gctaaggttc tgcacttcac tcaaggctcg
      181 gatgggatgc atccggctct tgccgatctc aataaggtct ttgacacgat tcagaaggca
      241 gggcatgtgc ggttgcacat cgctaactct ctctggcccc agaaagacca cccatttcag
      301 ccggattatc tcacgcttct caaagagcac tatggtgcat ccataacgcc ggtggactac
      361 gtcacagcaa cggaacaagc ccgcgagaaa atcaacaagt gggttgaagt caagacaaag
      421 gaaaagatca cgaatcttat tcccccgggc gttctgaacc atctcacccg cctcgtgctg
      481 gttaacgcga tctacttcaa aggggactgg aaaagccagt tcgatccgat caataccagg
      541 gaatctgagt tcaccgtttc gaagcatgac gaggttaaag tctccatgat gcatcagaag
      601 ggtcggtttg gatatgggga aactagtggt gcccaactgc tggaactccc ttacttgggg
      661 agcgatctat ccatggtggt gattctgcct cagacagtgg atcatctccc tgagattgag
      721 cgtcagctga ctgaggctaa tctgcatctg tggctttcaa gtctttctaa gcaggaagtt
      781 ctcgtctggt tgccaaagtt caaggtcact tggggcgtgt tcagtctcaa agagccacta
      841 cagtacctcg gaattcggaa ggcattcgac cccttgggtg ccgatttctc cgggatggac
      901 ggcacaaggg aactcttcat cactcaggta ctccacaagg catttgtgga agtcaacgaa
      961 gaggggacag aggcagccgc tgcgaccgct gcggtggtcg ggcgtaccat ggctctgcat
     1021 agaacacaaa catttaaggc cgatcacccc ttcctgtttc ttattcgtga caacgtgacc
     1081 ggaagcattt tgttcctcgg tagagtcctg aatccaagca agaattgagc gaatatcaag
     1141 aaagcccgtc tacagaacgg tatttgatag acagagagga caagggaccn nnnnnnnnnc
     1201 aatctcactc aagcctgcat cacaaggctg cccaagtggc agagaaagcc aagaaggctg
     1261 gagagaaacc ggcagatcag tgatcagatg ccgacatgag gcatggggta gctcgagagc
     1321 aagggcttat tcaccaaagg tgtttgttag cgaaagcgcg gccggatccg gatttcaggt
     1381 aacgctcaaa ttcagatgcg cgttgatcgt catcgaaccg gattgcggtt acaatgcgcc
     1441 acggcgcata tttggacgta tggggtgagc ctccttggtt gtgagtggtc aatcgct
//
LOCUS       DAOQUC010001171         1494 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_117283_length_1494_cov_3.767199, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001171 DAOQUC010000000
VERSION     DAOQUC010001171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1494
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117283_length_1494_cov_3.767199"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..728)
                     /locus_tag="PKH07_16590"
     CDS             complement(<1..728)
                     /locus_tag="PKH07_16590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003200233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HNT36607.1"
                     /translation="MFKKILIANRGEIALRIIRACKEMGIGTVAVYSEADRESAHVHF
                     ADESVCIGPAPSADSYLNITRIISAAEISDAEAIHPGYGFLAENADFAELCSSCDLVF
                     IGPSPDAISRMGDKAKARETMMAAGVPVIPGSEGVVRSTQEALELAHRIGYPVIVKAS
                     GGGGGRGMRVAHTDMTLGNVIITAQQEAQRAFGNAEVYMEKYIEQSRHVEIQIMGDSH
                     GNAVHLGERDCSIQRRHQKVLEESP"
     gene            complement(857..1357)
                     /gene="accB"
                     /locus_tag="PKH07_16595"
     CDS             complement(857..1357)
                     /gene="accB"
                     /locus_tag="PKH07_16595"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007129721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HNT36608.1"
                     /translation="MDYNLIKRIVALVERSDIHELEVEDEEVRIKVSKVTPVLEAAPR
                     LYAQDIGAITHMTPTPSLPTTHRGLQAATGQELSVPEESSQRNTITINSPMVGTFYRA
                     PAPEAPPFVKIGDSVDPDTVVCILEAMKVMNEIKAEVRGRITEVLVENAQPVEFGQAL
                     FRVEPN"
     gene            complement(1407..>1494)
                     /locus_tag="PKH07_16600"
     CDS             complement(1407..>1494)
                     /locus_tag="PKH07_16600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009267865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HNT36609.1"
                     /translation="AVVQVPLFIENGDVLKIDTRTGEYVERV"
BASE COUNT          320 a          446 c          372 g          356 t
ORIGIN      
        1 ggggattcct caagcacttt ctgatgcctt cgctgaatcg aacagtcgcg ttctcccaga
       61 tgaacggcgt ttccgtggga atctcccatg atctggattt cgacatgccg tgactgctca
      121 atgtactttt ccatgtagac ttcggcgttc ccgaaagcgc gttgcgcctc ctgctgggcc
      181 gtgatgatga cattgcccag agtcatatcg gtatgcgcga cacgcattcc gcgtccacca
      241 ccaccaccgg aagccttgac aatcaccgga tagccgattc ggtgagccag ttccaacgcc
      301 tcttgtgtgg aacgaacaac cccttcgctg cccggaatga ccggtacacc cgccgccatc
      361 atggtttcgc gcgccttggc cttgtctccc attcgagaaa tggcgtccgg cgatggaccg
      421 ataaacacaa ggtcacagga ggaacacaat tcggcaaaat cggcattctc cgccagaaat
      481 ccatagcctg gatggatggc ttccgcatcc gagatttccg ccgcgctgat gatccgagtg
      541 atgttcaggt agctgtctgc tgaaggtgct ggcccgatgc agacactctc atcggcaaag
      601 tgaacgtggg cactttccct gtctgcctcg gagtagacgg caacagtccc aatacccatc
      661 tccttgcagg cacgaatgat tcgaagtgca atctcacctc gatttgcgat taatatcttc
      721 ttgaacatga gcacagtctc cgactaaaga ttatcctaac acccagaaac cagagcccca
      781 ctttcctatc ggacttctcc tgctcgagta ttcgaccagc caggcatcgt ccacgcaccg
      841 ttcatgagag ccactctcag ttgggctcga ctcggaacag tgcctgaccg aattcgaccg
      901 gctgagcgtt ttcgacaagc acttcggtga tccgaccccg cacttcggcc ttgatttcat
      961 tcatgacctt catggcctcc aggatgcaga caacggtgtc tggatccacg ctgtctccaa
     1021 tcttcacaaa gggtggagcc tctggtgcag gagctcggta gaacgttccg accatggggg
     1081 agttaattgt gatagtgttt ctctgagaag attcctcagg caccgacagt tcctgacccg
     1141 tagcagcctg aagtcctcga tgcgtggtcg gcagagaggg agtgggtgtc atgtgagtta
     1201 tcgcgccgat gtcctgggca tacaggcgcg gcgccgcctc caagaccgga gtgaccttgc
     1261 tgactttgat ccgaacctcc tcatcctcaa cttccagttc gtggatgtcc gatcgttcca
     1321 ccaaagcgac gattctcttg atcaggttat agtccatttt gatccaccta tccttgggct
     1381 ttgccccgct gagggcaggg gaaccctcag accctttcta cgtattctcc cgttcgtgta
     1441 tctatcttca gaacgtctcc attctcgatg aaaagaggaa cctgaaccac ggca
//
LOCUS       DAOQUC010001172         1494 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_117296_length_1494_cov_3.357887, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001172 DAOQUC010000000
VERSION     DAOQUC010001172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1494
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117296_length_1494_cov_3.357887"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1494
                     /locus_tag="PKH07_16605"
     CDS             <1..>1494
                     /locus_tag="PKH07_16605"
                     /inference="COORDINATES: protein motif:HMM:NF025925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HNT36610.1"
                     /translation="PQSEREQQLFAEMPKPDEWHLSCVTSLEQDCTIVVPPESTVISS
                     ADKRSLGQDIRYDDPVYTKQFAQITPPGLDNGPLRSHTEALAEPFGFDAGDILYSALM
                     DLPHLFPYFRNSWDVTVVSDGTSVFCIESGDTRSRFFGLAFDIGTTSLVVSLVDLASR
                     QILGEMCASNPQAIWGADVIARIGHIQNDPQGLDQLRSAVLESLAEMVQKICRQFDVN
                     PLEVYAATFVGNTIMEHTLLGISPEAIATLPFHPVFRHEWVGRAERIRLPINPHGRVY
                     VSPVVSGYVGGDTLAVILATDLDERDELTLAIDLGTNGELALGHKNRIYACAAAAGPA
                     FEGGRLRCGMRAVPGAISSVRLGDDVEVTVLGDVDPIGICGSGLLDAVAEMVRLGIVE
                     DSGAMARPSHGVSKSIADRVIGEQEHALEFLLTHDGQRPVRITQKDIREFQLAKGAVR
                     AGCEVMMEEMGCKPEDVQRVLVSGAFGTHLRAESIAYVGLLPKFPRE"
BASE COUNT          323 a          391 c          440 g          340 t
ORIGIN      
        1 tccgcagtcc gagcgcgagc aacaactgtt cgcagaaatg ccaaagcctg acgagtggca
       61 cctgtcatgc gtgacctcat tggagcagga ctgcaccatc gtagttcctc ccgaaagcac
      121 tgtcatctcc agcgccgaca aacgttctct gggacaggat attcgctatg acgaccctgt
      181 ctacaccaag cagttcgcgc agatcacacc acccggtttg gacaatggcc cattgcgctc
      241 acacacggag gctcttgcag agcctttcgg atttgacgcg ggggacatac tctattcggc
      301 gctgatggac ttgccccact tgtttcctta cttccgcaac agctgggatg tgactgttgt
      361 atcagacgga acgtcggtct tctgtatcga gtcgggagat acgcggagcc gcttctttgg
      421 attggcgttt gacatcggaa caacttctct ggtggtttcc ctggtagacc tggcttcacg
      481 ccagatcctc ggagaaatgt gtgccagcaa tccccaggca atctggggag ccgacgtaat
      541 cgcacgaatt ggccacattc agaacgatcc ccaaggcctg gatcaactgc gctccgcagt
      601 gctggaatca ctcgcagaga tggtgcaaaa gatttgtcgt cagtttgatg tgaatcctct
      661 cgaagtctac gcggcaacgt ttgttggcaa cacaattatg gaacacacgc ttcttgggat
      721 ttcgcctgaa gcgattgcta cgctgccttt tcatcctgtt ttccgccatg aatgggtggg
      781 tcgggcagag cggatcagac ttccgatcaa cccgcacgga cgagtctatg tgtctcctgt
      841 ggtttccggt tacgttggtg gggatacgct cgcagtcatc ctggcaacag atttggacga
      901 aagggacgag ttgaccctcg ccattgatct gggaaccaac ggggaactgg cactcggtca
      961 caagaacagg atctacgctt gtgccgccgc ggcaggaccg gccttcgaag gaggacggct
     1021 ccgctgtggc atgagagctg ttccgggagc gatcagtagt gtacgactcg gggacgacgt
     1081 tgaggtaact gtcctgggtg atgttgatcc aattggcatc tgcggttcgg gacttctgga
     1141 tgctgtagcc gaaatggtgc ggcttggaat tgttgaagat tccggagcga tggctcgacc
     1201 gagtcatggc gtatccaaga gcattgctga ccgtgtgatt ggcgagcagg aacatgctct
     1261 ggagttcttg cttacgcacg atggacagcg acctgtccgc attacccaga aggacattcg
     1321 ggagtttcag ctcgccaaag gagccgtgcg cgcgggatgt gaagtcatga tggaagagat
     1381 gggatgcaag ccagaagacg tgcagcgcgt tttggtctca ggtgcctttg gaacacacct
     1441 gcgcgcggag agcattgcat acgtcggact gctgccaaaa ttccccaggg agaa
//
LOCUS       DAOQUC010001173         1494 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_117375_length_1494_cov_2.129256, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001173 DAOQUC010000000
VERSION     DAOQUC010001173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1494
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117375_length_1494_cov_2.129256"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..809
                     /locus_tag="PKH07_16610"
     CDS             <1..809
                     /locus_tag="PKH07_16610"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNT36611.1"
                     /translation="LPMDHPVKTMDDWIKIKPWYEFSERRFGADWQAKATRIRNEDRV
                     VCVAIPGGFDEPRQLLGEENLCIAYYEQPELVHDILSTIGDTALKVLDRVSAAVPVDM
                     LCVHEDMAGRSGSLAGPKQIREFIAPYYRKIWNMLAERDARLFDQDSDGDMNSVIDAF
                     LEAGVNCMHPMEPAANMDIVGVRKKYGTRLAFYGGIDKHIIRQSKEDIVRELEYKIPP
                     MVKTGGCVLALDHRIPAGTPLENYKFYLRKAWEIMDRESSRCQSSRQCSV"
     gene            complement(818..>1494)
                     /locus_tag="PKH07_16615"
     CDS             complement(818..>1494)
                     /locus_tag="PKH07_16615"
                     /inference="COORDINATES: protein motif:HMM:NF036773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl hydrolase-related protein"
                     /protein_id="HNT36612.1"
                     /translation="QRNINWLVTKDNHEKHYPIQPMMHFLDLSDGDNSLALMTRGLHE
                     YEVIDDPRRTIALTLLRTHRAYMLANRGLMTPEEYERNKGQHCIGTVEMEYALMPHQS
                     DWQQAQITHQSEDFRTPWRIIQGVPKPGKEPVTQSLFEISPSDRIRISALCQSEDGKA
                     FLLRVWNCSGDSVQMKLKLPQTVSSVEKVHMDESKSLARLSKKDGRWMATLRSKEIAT
                     FRLQLS"
BASE COUNT          334 a          374 c          425 g          361 t
ORIGIN      
        1 cccttccgat ggatcatccg gtcaagacca tggatgactg gatcaagatc aagccgtggt
       61 atgaattctc ggagaggcgg ttcggtgctg actggcaggc caaagccaca cgcatccgca
      121 acgaagaccg tgttgtctgt gttgccattc ccggtgggtt cgatgagcca aggcagctgc
      181 tcggtgaaga gaatctttgc attgcctact acgagcaacc cgagttggtt cacgacattc
      241 tgagcaccat cggagacact gcgctcaagg tactggatcg ggtttcagcg gctgtccctg
      301 ttgatatgtt gtgtgtgcac gaagacatgg cgggccgatc ggggtcattg gctggcccga
      361 aacagattcg agagttcatt gctccctact atcgaaagat ctggaacatg ttggcagaac
      421 gtgacgcacg gctttttgat caagacagtg acggagatat gaattcggtg atagacgctt
      481 ttctcgaagc gggtgtcaat tgtatgcatc ccatggaacc agccgccaac atggacattg
      541 tcggggttcg caagaagtac ggcacacgct tggcattcta cggtggcatt gacaaacaca
      601 tcatccgtca gtcaaaggaa gacattgtca gggaattgga gtacaagatt ccacccatgg
      661 tcaagaccgg gggatgtgtt cttgccctgg atcaccggat tcccgccgga acgccgctgg
      721 agaactacaa gttctatctt cggaaagcgt gggaaatcat ggatcgggaa tcctcacgct
      781 gccaaagctc tcgccagtgt tccgtgtgat ggtgtcttca ggatagctgc agtcgaaacg
      841 tggcgatttc cttgcttcga agagttgcca tccatctgcc gtccttcttg ctgagtcgtg
      901 caagagactt tgactcatcc atgtgcactt tctcgaccga agagaccgtc tgcggcagct
      961 tgagtttcat ctggactgaa tcgccagaac agttccaaac ccgaagcaga aacgccttgc
     1021 catcttcgga ctgacagagc gctgaaatcc tgattcgatc ggatggcgaa atctcaaaga
     1081 gactctgagt gacaggctcc ttgccaggct tggggacacc ctgaatgatg cgccagggcg
     1141 tcctgaagtc ctctgactgg tgagtgatct gtgcctgctg ccagtcagat tgatgaggca
     1201 tcagagcgta ctccatctcg actgttccaa tgcagtgctg tcctttgttg cgttcgtact
     1261 cctcgggagt catcaagcct cggttggcca gcatgtaggc acggtgcgtt cggaggaggg
     1321 tcagggcgat ggtgcgtcgt gggtcgtcaa tgacctcgta ctcatgaagt ccccttgtca
     1381 ttagagcgag agaattgtcg ccgtcactca aatcgagaaa gtgcatcatc ggctggatag
     1441 gatagtgctt ctcgtgattg tcctttgtga caagccagtt gatgttccgc tgga
//
LOCUS       DAOQUC010001174         1491 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_117682_length_1491_cov_3.291086, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001174 DAOQUC010000000
VERSION     DAOQUC010001174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1491)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1491)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1491
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117682_length_1491_cov_3.291086"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1148)
                     /locus_tag="PKH07_16620"
     CDS             complement(<1..1148)
                     /locus_tag="PKH07_16620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HNT36613.1"
                     /translation="MIVSREQLEAWERSILMPCAQASADSLGRQHPEQEHPLRTRFQR
                     DKDRIIHSTAFRRLEYKTQVFVNHEGDYYRTRLTHTMEVAQIGRTIARSLRLNEDLVE
                     AVSLAHDLGHTPFGHSGEDILQELMKDHGGFEHNAQGLRVVDLLENRYPEFPGLNLTS
                     EVREGIIRHSTPWDRAALPAVGIPVSFPSGGRLLETQVVDIADEIAYNSHDVDDGLES
                     GLLSFEDLREVPLWQKIMEHVESRFADLSLKKKRHIYVRTMINFQVTDVIEESARKLE
                     SSSLQSVSDVRERGDSLVGFGQEMSLHNEQLKKFLWENLYKHQKVVRMADKAKRIISD
                     LFNLYVNKPDQLPPLFQERASKESVHRVVSDYIAGMTDRYAYEDYRDL"
     gene            complement(1158..>1491)
                     /locus_tag="PKH07_16625"
     CDS             complement(1158..>1491)
                     /locus_tag="PKH07_16625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007223360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HNT36614.1"
                     /translation="VDETIMTRHDVPKVAGICDGVNLKLEKCGGIEEGLAIIQTARAH
                     RLGVMIGCMVGSSVDITAAAHLTPLVDYADLDGHILTANDPYEGMTVQDGWMLIPDRP
                     GLGVVKRG"
BASE COUNT          323 a          446 c          375 g          347 t
ORIGIN      
        1 aacagatctc ggtagtcttc ataggcatag cggtcggtca tgccggcaat gtaatcggag
       61 accacccgat gcaccgactc cttgctggcg cgttcctgaa acagcggtgg caactggtcg
      121 ggtttgttca cgtagagatt gaacagatcg ctgatgatcc ttttggcttt gtccgccatt
      181 cgcacaacct tctgatgctt gtagaggttt tcccaaagaa acttcttcag ttgttcattg
      241 tgaagtgaca tctcctggcc aaacccaacc aacgaatccc ctcgctcgcg aacatccgaa
      301 acactctgga gactgctgct ctcgagtttc cttgcactct cctcgatgac atcggtcacc
      361 tggaaattga tcatcgttcg gacatagata tgtcgcttct tcttgagact cagatcggca
      421 aatcgggatt ccacatgctc catgatcttc tgccagagag gaacttccct gagatcctcg
      481 aaagacagaa gccccgactc caagccgtcg tccacgtcgt gtgagttgta ggcaatctca
      541 tcggcgatgt ccacaacctg cgtttccaga agcctgccac ctgaaggaaa cgagacgggg
      601 attcccactg cgggaagtgc cgcacgatcc caaggtgttg aatgccggat gattccctca
      661 cgcacttccg aagtgagatt caatcctggg aactccggat atcggttctc cagcagatcc
      721 acaactcgaa gcccctgtgc gttgtgttca aagccaccgt gatccttcat cagctcttga
      781 aggatatcct ctcccgaatg accgaaggga gtatgtccca gatcgtgcgc gagagaaacc
      841 gcctcaacca gatcctcgtt caaccgaagc gatcgggcga tcgtccgccc gatctgggca
      901 acttccatgg tgtgagtcag tcgggtgcga tagtaatctc cctcatgatt gacaaaaacc
      961 tgggtcttgt actccaaacg ccggaaggcg gtcgaatgaa tgatgcggtc tttgtcgcgc
     1021 tggaatcggg ttcgaagcgg atgctcctgt tccggatgtt ggcgtccgag gctgtcggcg
     1081 cttgcctgag cgcacggcat caggatcgag cgttcccatg cttccagttg ttccctgctg
     1141 acaatcaccg acatctctca gcctcgcttc acgacaccca gcccgggacg gtctggaata
     1201 agcatccagc cgtcttgaac cgtcatgcct tcatacgggt cgttggcggt caggatgtgt
     1261 ccatcgagat ctgcgtaatc caccagaggt gtcaggtgcg ccgccgcggt aatgtccacc
     1321 gaggaaccga ccatgcagcc gatcatcact ccaagcctgt gtgcccgcgc tgtctgaatg
     1381 atcgccagac cttcctcgat gccaccgcac ttctccagtt tcagattcac accgtcgcaa
     1441 atccccgcca ccttgggaac gtcatggcgc gtcatgatcg tttcatccac a
//
LOCUS       DAOQUC010001175         1491 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_117698_length_1491_cov_2.859331, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001175 DAOQUC010000000
VERSION     DAOQUC010001175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1491)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1491)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1491
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117698_length_1491_cov_2.859331"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..677
                     /locus_tag="PKH07_16630"
     CDS             <1..677
                     /locus_tag="PKH07_16630"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNT36615.1"
                     /translation="SIRHNTLALDGKRLYLIDRPLAERDRLDFKKDEQQEPPQQPGGE
                     LIALDIQTGKSVWQVAEDIYGTLLIVSAKHDALLMPYQITSFKLPSEVGGKMAAFRAS
                     DGTKLWDIQANYKSRPIVNDRTIYAQPDSWDLLTGERREMKFARSYGCGTLAGSTYLL
                     AYRSATLGYWDLRTGTETINYGGIRPGCWINAIPAGGLLLMPEASNRCTCSYLIKANV
                     ALQPAH"
     gene            704..1174
                     /locus_tag="PKH07_16635"
     CDS             704..1174
                     /locus_tag="PKH07_16635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36616.1"
                     /translation="MDYEKTVPFEGNLSKVIELATSVFTPFGLAIASRSDSSVEFTGA
                     NLWITSQNPFVAISRIRIQGTGTQLTLTADLKGLQRLFRFVGLMLCGMALAFVLVFGL
                     FFRQGDLLTRVVLPVLPFVPWIVLFPILKYVIRSRTCQALDILLQNLTIVGRSA"
     gene            1178..>1491
                     /locus_tag="PKH07_16640"
     CDS             1178..>1491
                     /locus_tag="PKH07_16640"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HNT36617.1"
                     /translation="MKDNRLAKGFLLMMGANVTFCVMSGLIRNASNIDAYKTTLFRFI
                     IGLALLGVAAIAGKIKLRFVNIRFLFLRGIFGGLAVFFFFLSIAKLGVGKGTVLNSLY
                     PI"
BASE COUNT          326 a          386 c          394 g          385 t
ORIGIN      
        1 attccattcg tcacaacacg cttgccctgg acggcaagag actctatctc attgaccgtc
       61 cactggcaga gagagatcgc cttgatttca agaaggacga acaacaggag cctccgcagc
      121 agcctggcgg ggaactcatc gcattggata tccagacggg caaatccgtc tggcaggtgg
      181 cggaagacat ctacggtacg ctactgatcg tcagtgccaa acatgacgca ttgcttatgc
      241 cgtaccagat cacaagtttc aaacttccct ctgaggtcgg tggcaagatg gcggcattcc
      301 gcgcttcgga tggaaccaaa ctctgggaca ttcaggcaaa ctacaaatcc cgaccgatcg
      361 tcaatgaccg aaccatctac gcacaacctg attcatggga tcttctgaca ggggaacgca
      421 gagagatgaa attcgcgcgt tcgtatggct gcggaactct cgctggctcc acctaccttt
      481 tggcttatcg ctcagcgaca ctgggctatt gggatctcag gacaggtacg gagacaatca
      541 attacggtgg catcagaccc ggatgctgga tcaatgcgat tccggcgggt ggtctgcttc
      601 tgatgccaga agcatccaat cggtgcacat gcagttatct gatcaaggca aatgtggcgc
      661 ttcagccagc acactaggct gtctgcgcct ggaggttgtc cggatggact atgagaaaac
      721 tgttcctttt gaagggaatc tgtcaaaggt catcgaactt gccacaagcg tgttcactcc
      781 ctttggcctt gccattgcca gcagaagcga ttccagcgtg gagttcaccg gcgcgaacct
      841 gtggatcacc agccagaatc catttgttgc gatctcgcgg attcggatcc aaggcactgg
      901 cacgcagctg acgctgaccg cggatttgaa aggtctccaa agactgttca ggtttgttgg
      961 attgatgctc tgcgggatgg ctctggcttt tgtcctcgtc tttgggttgt tttttcgaca
     1021 aggtgacttg ctgacaagag tggtgctgcc tgtgctgcct tttgtcccct ggatagtcct
     1081 ttttccgatt ctgaaatacg tcatccgatc tcgaacctgt caggcattgg atatcctgct
     1141 tcagaacctg actatcgtgg gaaggtcagc gtagaggatg aaggacaatc gccttgccaa
     1201 gggttttctc ttgatgatgg gggcaaacgt caccttctgt gtgatgtcag gtctcattcg
     1261 aaatgcttcc aacattgatg cgtacaagac gacactcttt cgtttcatca taggcctggc
     1321 tctgctgggc gtcgcggcaa tcgccggtaa gatcaaactg agatttgtca acattcgttt
     1381 tctgtttttg cgtgggattt tcggcgggct ggctgtgttt ttctttttcc tgtccatcgc
     1441 caaactcggc gttggcaaag gaaccgtgct caactctctc tacccgatct t
//
LOCUS       DAOQUC010001176         1490 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_117866_length_1490_cov_2.389547, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001176 DAOQUC010000000
VERSION     DAOQUC010001176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1490)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1490)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1490
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117866_length_1490_cov_2.389547"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..643)
                     /locus_tag="PKH07_16645"
     CDS             complement(<1..643)
                     /locus_tag="PKH07_16645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36618.1"
                     /translation="MSDPSSVSIQAGRTRFVFEPMTQRFDALCHGRTLTFQNLLPLFA
                     TEEGDSATEAESSDSTLFASGRDGNEYWFDYRSGINGASHEYRIGISVRDGSLRIRLS
                     ADGQAGRGVLAPRVESMGTWRRVETSQYGLAHGQTWYQGAWYFDDVRLWCWSFWDLDA
                     GNASSWGEQTMPNSGSGNVRMASSVLYQPNADGVYQPLRETLMIRVAERLWPAV"
     gene            complement(775..>1490)
                     /locus_tag="PKH07_16650"
     CDS             complement(775..>1490)
                     /locus_tag="PKH07_16650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HNT36619.1"
                     /translation="TYRNFLAQFLPLFRNFLIQEGIMDKSLFHLSDEPHGDEHLENYR
                     RARALLAELAPWMKVMDALSDARFAQQGLTDIPVASIGAAKSFREAGIPCPVYFCCGP
                     RGRYLNRFLDTPLSKIRMSGWLFYRLKALGFLHWGYNYWCKSQQQELVDPFTESSATF
                     WPRWTYGDPFVVYPGADGPIDSIRWEVFGESLQDFALLQTAGVDPDDPMLADIREYDD
                     FPKSESWIRQLREWILLDR"
BASE COUNT          392 a          444 c          356 g          298 t
ORIGIN      
        1 gaacggcagg ccagagtcgc tcagcgactc ggatcatcaa agtctcccga agaggctgat
       61 acacaccatc cgcattgggt tgataaagta ccgaggacgc catccgaaca tttcccgacc
      121 ccgagttcgg catcgtctgc tcaccccagg atgacgcatt ccccgcgtcc aagtcccaga
      181 aactccaaca ccaaagtcgc acatcgtcga aataccaggc tccctgatac caggtctggc
      241 catgcgcaag accgtactga ctggtctcaa cacgacgcca ggtccccatg ctttccactc
      301 ttggtgccag aactccgcgt cccgcttgcc cgtctgcact caaccggatc cggagtgaac
      361 catcacgaac ggatattcca atccggtatt catggcttgc tccattgata ccagaacggt
      421 agtcaaacca gtactcattg ccgtcccgtc ctgaagcaaa cagcgtggag tccgatgatt
      481 cggcctcggt tgcggagtcg ccttcttccg tcgcgaaaag tggaagcaga ttctgaaaag
      541 tcagcgttct gccatggcac agagcatcga aacgctgggt cataggttca aaaacaaacc
      601 ttgttcgtcc tgcctgaatt gaaacggaac tgggatcaga cattggttcc ccctttccga
      661 aggccacaca gcactgtagt gcaaaagcgg ccagtgcaag caaaaagaaa cgagatcggc
      721 aacacctgat catcaaccat ccaggaaagc aaagatttcc gacaagtttt gacatcatct
      781 gtcgagaaga atccactccc tgagctgtcg aatccatgat tcggatttcg gaaaatcatc
      841 gtactctcga atgtccgcaa gcatcggatc atccggatca accccagccg tttgaagcaa
      901 ggcaaaatcc tgcaaggact ctccaaacac ttcccaccga attgagtcaa ttggtccatc
      961 cgctcctggg taaacgacaa acggatctcc ataagtccag cgcggccaga acgtcgccga
     1021 agactccgtg aagggatcta ccagttcctg ctgctgcgac ttgcaccagt agttgtagcc
     1081 ccaatgaaga aaaccaagcg ccttgagccg atagaaaagc caccctgaca ttcggatctt
     1141 gctcagaggt gtgtcgagaa aacggttcag atacctgcct ctgggaccac aacagaaata
     1201 gaccggacag ggtatgccgg cttcccgaaa cgattttgcg gcgccgatgc tggcaacagg
     1261 gatgtccgtc aacccctgct gcgcaaagcg cgcatcactg agtgcgtcca taaccttcat
     1321 ccacggtgcc agttccgcaa gcagcgcccg tgccctgcgg tagttctcca agtgctcgtc
     1381 tccgtgaggc tcatcagaca ggtgaaagag cgacttgtcc atgattcctt cctggatcag
     1441 gaaattcctg aacaaaggaa gaaactgcgc cagaaaattc cgataggtgt
//
LOCUS       DAOQUC010001177         1489 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_117930_length_1489_cov_4.294979, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001177 DAOQUC010000000
VERSION     DAOQUC010001177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1489
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117930_length_1489_cov_4.294979"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="PKH07_16655"
     CDS             <1..204
                     /locus_tag="PKH07_16655"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNT36620.1"
                     /translation="LDNGQGMTDEVLGQLFQRGFSTKQGEKAGPGLHWCANVLRMLGG
                     SIRAESQGSGHGTCLHLVLPAAD"
     gene            243..1331
                     /locus_tag="PKH07_16660"
     CDS             243..1331
                     /locus_tag="PKH07_16660"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNT36621.1"
                     /translation="MGDHLRILAADDEPSVLSLYGEILSPSQASDGDDVMAALEAKIF
                     GVGQADVPVGPVFDLVLCSQGDEALEKIRSSIDDEDPFSAAFLDVRMPPGPDGVSVAE
                     SIRVLDPFINIVIVTGYSDVDPLEIARRVPPADKLLYVQKPFHVHEIRQFASALTAKW
                     LLERRLAIIMSELEQKIEERTKELIKVNQELVDTNKVLEKLSLTDDLTKLYNKRYFNK
                     KIAEWVTYSKRYKAPLSLIMFDIDHFKQYNDLKGHLMGDLLLEKLGQLVQHSLRRQID
                     TAFGGIESSYRFGGEEFAIILPRTDRSGAHIVAERLRSNVEKETGVTISVGVTDFDEE
                     KDDAASLVHKADLNLYEAKKRGRNCVVS"
BASE COUNT          343 a          385 c          408 g          353 t
ORIGIN      
        1 ctggataatg gtcaaggcat gacagacgag gttctgggac agttatttca gcggggattt
       61 tcgactaagc agggagagaa ggccggccct ggattgcatt ggtgcgccaa tgttctcagg
      121 atgcttggtg gttcgattcg agccgagagc caaggctcag gccacggtac ctgtcttcat
      181 cttgtgcttc ccgccgccga ctgacccaag cctggggtgc gcttggcata aggattgacc
      241 taatgggtga ccatctcagg atactggcag cggatgatga gccgtccgtg ctcagtctct
      301 acggtgagat tctctctcct tctcaagcca gtgacggtga tgacgtgatg gcagctctgg
      361 aggcaaaaat cttcggggtt ggccaagcgg acgttccggt gggtcccgtt tttgacctgg
      421 tcttgtgttc gcagggggat gaagcccttg agaagattcg gtcctctatt gacgacgaag
      481 atcccttctc ggcggccttc ctggatgtcc gaatgcctcc tgggccggac ggcgtttctg
      541 tcgccgagag cattcgcgtt ctggatcctt tcatcaatat cgtcattgtc acgggttact
      601 ccgatgtaga tcccctggag attgcccgtc gtgttccgcc agcggataag ttgctttacg
      661 tacagaagcc ttttcatgtg catgagattc ggcagtttgc ttctgccttg acggcaaagt
      721 ggctcctgga gaggaggctt gccatcatca tgtccgagct ggagcaaaag attgaagaac
      781 gcaccaagga attgatcaag gtcaatcagg aactcgttga caccaacaaa gtcctggaaa
      841 aactctcact taccgatgac ctgaccaaac tctacaacaa gcgatatttc aacaagaaga
      901 ttgccgaatg ggtcacctat tccaagagat ataaagcccc actttcactc atcatgttcg
      961 acattgacca tttcaagcaa tacaacgatc tcaaaggtca tctcatgggg gatctcctgc
     1021 tggagaaact gggtcagctg gtccagcact ccctgcgtcg gcagattgac acggcctttg
     1081 gtggaatcga atccagctat cgtttcggcg gagaggaatt cgcgatcatt ttgcctcgaa
     1141 ccgatcgcag tggagcccac attgtcgccg agaggcttcg ctccaatgtc gagaaagaga
     1201 ctggcgttac catcagcgtt ggcgtgaccg actttgacga ggaaaaggat gacgcagcaa
     1261 gcctggttca caaggcggac ttgaatctct acgaggccaa gaaacgtggc cgaaactgtg
     1321 ttgtttcctg agaagagcgg ccgcgagtca ctctcgattc atcccaatca cacactccaa
     1381 gcacgtccaa gcgtatccgc tcagttacac agggtatggt tgtcattctg aatccttcgg
     1441 cggagtctat cctgagcaaa gccgaaggac tcaggacagg ttccgcgac
//
LOCUS       DAOQUC010001178         1489 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_117968_length_1489_cov_2.955370, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001178 DAOQUC010000000
VERSION     DAOQUC010001178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1489
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117968_length_1489_cov_2.955370"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="PKH07_16665"
     CDS             <1..250
                     /locus_tag="PKH07_16665"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNT36622.1"
                     /translation="HLYVIRTPQREALREKLIGKQIGVAIHYPIGIHEQVAFRNYSKQ
                     QFSLPVTESVCKEVLSLPMYPELSAEQIEYVAACVTNP"
     gene            complement(330..1130)
                     /locus_tag="PKH07_16670"
     CDS             complement(330..1130)
                     /locus_tag="PKH07_16670"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNT36623.1"
                     /translation="MRLRMMIVGLVLLITTGCFAEVRDNMFSERFERRQGALCRGFRD
                     SKEMALVFTGGEFGEGLETILNELKSRNLKGAFFFTGAFFKIDAHQPLIRRLVAEGHY
                     LGPHSEEHLLLASWEEDKTLVTREQFIEDLDRNVARCVEYGFKREDIRYWIPPYEQTN
                     EEVSRWSQEHGLTMINFTRGTLSHTDYAPEDDKHFRPTEVVIQSIKDYPSKDPDGFNG
                     FLLLTHVGAGARKDKLHPHFGSIVDYLLGRGQIIVRVDELLDKGRGVQ"
     gene            complement(1152..1397)
                     /locus_tag="PKH07_16675"
     CDS             complement(1152..1397)
                     /locus_tag="PKH07_16675"
                     /inference="COORDINATES: protein motif:HMM:NF020967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HNT36624.1"
                     /translation="MNDEMKVVYQGKITDVDFVRMLLEDEGIETVLTNEGFSFYLDSA
                     CPGVPPGVAVQDTDAERATEIVADFVSKQRQPPKEDE"
BASE COUNT          322 a          427 c          371 g          369 t
ORIGIN      
        1 tcacctctac gtcatccgaa cgccccagcg cgaggcactc agggagaaac tgatcggcaa
       61 gcagatcggt gtggcgattc actatccgat cgggatccat gagcaggtgg cctttcgcaa
      121 ctactccaag cagcagttct cactgcccgt aacagagtca gtctgcaaag aggttctctc
      181 gctgcccatg tatccggaac tgagtgccga gcagattgag tacgttgcag cctgcgtcac
      241 aaatccctga cacgaaacga tgggattgtg ctgcaacgga agctggctgt ggtaagacct
      301 gcacgcgaaa gtcacgtgca ccccgcttct tactgaacgc ctcgtccctt gtccaggagt
      361 tcatctactc ggacgatgat ctgtcctcga cctagcaggt agtccacaat gctgccgaaa
      421 tgcggatgca gtttgtcttt tcgggcacct gcgccgacgt gcgtcagaag caggaatccg
      481 ttgaagccgt ccggatcttt gctggggtag tctttgatgc tctgaatcac cacttcggtc
      541 ggtcgaaaat gcttgtcgtc ttcaggagca tagtccgtgt gactcagagt gccccgtgtg
      601 aagttgatca tggtgagtcc gtgttcctga ctccatcggc tgacttcctc attcgtctgc
      661 tcatagggtg gaatccagta acggatatcc tcccttttga aaccatattc gacacagcga
      721 gcgacgttgc ggtccagatc ttcgatgaac tgctctcggg tcaccagggt cttgtcttct
      781 tcccagctgg cgagcaaaag gtgttcttca ctgtggggtc ccagataatg gccttctgcg
      841 accaaccgac gaatcagtgg ctgatgtgcg tcgatcttga agaatgcgcc tgtgaagaag
      901 aaagcccctt tcagattacg acttttcagc tcattcagaa tcgtctccag accttcaccg
      961 aattcaccac cggtgaacac aagcgccatt tccttggagt cccgaaaacc acgacacagc
     1021 gcgccctgtc ttctctcgaa ccgttcactg aacatgttat ctctcacctc cgcaaagcat
     1081 cctgttgtga taagaagcac caaaccaacg atcatcatcc tcagacgcat ctttggaaac
     1141 ctcctgagcg ttcactcatc ttctttcggt ggctgccttt gctttgacac aaagtccgcc
     1201 acgatttcgg ttgcccgctc ggcatcggtg tcttggactg ccacgcctgg tggcactcca
     1261 gggcaggcgc tgtcgagata aaaggagaac ccctcattgg tcaggactgt ttcgatccct
     1321 tcatcttcga gaagcatacg gacgaagtcc acatctgtga ttttgccctg atagacaacc
     1381 ttcatctcat cgttcacgag ttggggctcc agtctcgggt tttcccttgg gacttcggaa
     1441 gatgatttca tgaatcttca ctggcgagcg ctgatcccga gactggagc
//
LOCUS       DAOQUC010001179         1488 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_118094_length_1488_cov_2.865318, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001179 DAOQUC010000000
VERSION     DAOQUC010001179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1488
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118094_length_1488_cov_2.865318"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1369
                     /locus_tag="PKH07_16680"
     CDS             <1..1369
                     /locus_tag="PKH07_16680"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNT36625.1"
                     /translation="VTGCYGDPIVQTPNLDRLASQGIVFENAYTTSPLCVPARQSFTA
                     GKYASRVSAWNNHRMMPPDSPSLPRLLNACGYQSVLVGKMHYDQTCRYGFEELPTKSN
                     NGIMMGTGKRRLYDDTSTNPKSWESRAKSFVAGEDSRIISHDKDVTRQASEFLGKRKK
                     DDPPFFLLAGYLAPHFPLTVPEEYLSRYRGKVPMPNIPEGLIEQLPTNYKHIRMGFGV
                     VDTDPETVQLGRECYWAFVNWMDDQVGELLRALEQSEVGQDTVVIYMTDHGENKGDHG
                     MWWKSCMYEHAAGIPLIISCPGRLPQGQRRRGACSSVDVVQTIADIAGADSPADWDGD
                     SLLPWIEDPQFAWKDFALSEYYAHFILSGFTMVRQGKYKYVYHARWDEKRPPELELYD
                     MEQDPGELENLIGDPHYADTAASMHARMIKELGEHPDDIEKRAVKELAQGYNRPEKGK
                     AESEE"
BASE COUNT          367 a          397 c          416 g          308 t
ORIGIN      
        1 cgtcacgggc tgctacggag accctattgt gcagacgccc aacctggatc gtctggcatc
       61 gcagggaatc gtctttgaga atgcctacac gacctctccc ctttgcgtgc cggcacgtca
      121 gagttttacc gcgggcaagt acgcaagccg agtcagcgcc tggaacaacc accggatgat
      181 gccacccgac agcccttcac tgccacggct gctcaatgcc tgtggctatc aaagtgttct
      241 tgtcggaaag atgcactacg accaaacctg ccgttacggc ttcgaggaac tccccaccaa
      301 gagcaacaac ggaatcatga tgggcacggg gaaacgacgc ctgtatgacg acacttccac
      361 caatcccaag tcttgggagt ctcgtgcgaa gagttttgtt gccggagaag actctcgcat
      421 cattagccat gacaaggatg tgactcgaca ggcaagcgag tttcttggaa aacgaaagaa
      481 agatgatccg cccttctttc tgctcgcagg atatctggca cctcatttcc cgcttacagt
      541 tccggaggag tacttgtctc gctatcgagg caaggtaccc atgcccaata taccggaagg
      601 tctcattgag caacttccaa ccaactacaa acacataaga atgggctttg gagttgtgga
      661 caccgatccc gagacggttc agctgggacg ggagtgttac tgggcctttg tgaactggat
      721 ggatgatcag gttggcgaac tgcttcgagc actcgaacag tctgaggtgg gacaagacac
      781 ggtggttatc tacatgactg atcacggtga gaacaaaggc gatcacggca tgtggtggaa
      841 gagttgcatg tatgagcacg cggcggggat cccgctcatc atctcctgcc cggggcgtct
      901 cccacaaggt cagagacgtc ggggagcctg ctccagtgtg gatgtcgtgc agaccatagc
      961 ggacatcgca ggcgctgact ctcctgcgga ttgggatgga gactccttgt taccatggat
     1021 tgaagatcct cagttcgcgt ggaaggactt tgcactcagt gagtactacg cgcatttcat
     1081 cctctccggc ttcaccatgg ttcggcaggg caagtacaag tatgtctacc atgcgcgttg
     1141 ggatgagaag agaccaccgg aacttgagtt gtacgacatg gagcaggatc ccggtgaact
     1201 tgagaacttg atcggagatc cccactatgc agatactgcc gcctcgatgc atgcacggat
     1261 gatcaaagaa ctcggcgagc atcccgacga tatcgaaaag cgagccgtga aagaactcgc
     1321 ccaaggctac aaccgacctg agaagggcaa agccgagagc gaggaataag gcgcgtggct
     1381 cggcgtactg gttcagctac acgaggtgtc taagacactc gtcattcaga attcttcgaa
     1441 gactcaggac aggctccaca agtcttcgag cggagtgaag aatctctc
//
LOCUS       DAOQUC010001180         1485 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_118494_length_1485_cov_3.242657, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001180 DAOQUC010000000
VERSION     DAOQUC010001180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1485
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118494_length_1485_cov_3.242657"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            232..1320
                     /locus_tag="PKH07_16685"
     CDS             232..1320
                     /locus_tag="PKH07_16685"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNT36626.1"
                     /translation="MTKMRKATLIFTLFTITMGCGSPDDTVSAKAPQPNQPLSLETPT
                     PAGPAERDTDPRVVLKINGDPITMGEINTMAYAVLHQSGLSPYSPEGQKALLSIRPNI
                     LNTLLSLRLLKQGAKKLKVKMDPSLVEKHIEAVKSRYSSEKEFLRSLEKEGMTLEAYR
                     NNVTDEIIVQTVKDRIMGEGVVEPTDEEVEEFYNAHLKDFKKPEMIHLCLLMIGYPEN
                     PAMEQKSEAFSIINRLYFRILAGENFEELATTYSTGPNKEKGGDLGYKPREYVQKYLE
                     NAPEYQLAAGNVTPVLETPTGYVLLYPKEIVPKKDVSLEQASQEIRGILKDRQKARNL
                     QRWLNSQTESAKMEVVDPDLYKLVSVSQ"
     gene            1369..>1485
                     /locus_tag="PKH07_16690"
     CDS             1369..>1485
                     /locus_tag="PKH07_16690"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007936029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HNT36627.1"
                     /translation="MPAVVVVGLQWGDEGKGKIVDFLSREAQLVIRHQGGNNA"
BASE COUNT          409 a          353 c          412 g          311 t
ORIGIN      
        1 cggcatacac cgtcctttgg gcattctcgt ggcggccgca aagggagcgg tactggttga
       61 agaggttcag ccagagaata aaccgaaaat gaatgcatgt tcttacgcaa acgggtatga
      121 tattcgtctg agtcatattt ttgcctgaaa ccctcgtgct cgaaagggtc tgcttctgag
      181 gaaacagacg gagccaagga gattcaggaa gtgtgtaagg tgaatgtacc aatgacaaaa
      241 atgcgaaaag cgacgctgat cttcactttg ttcacgataa ccatgggttg tggcagcccg
      301 gacgataccg tcagcgccaa agccccccag cctaaccagc cgttatcgct ggagacgcca
      361 acaccagcag ggcctgccga gcgagacacc gatccaaggg tggtgttaaa gatcaatggc
      421 gacccgatca ccatgggcga aatcaacacc atggcgtatg cggtgctgca ccaaagtggc
      481 ctctctccct attctcccga gggacaaaag gcgctgttga gcattcggcc gaatatactc
      541 aacacgctgc tttccctgcg tcttttgaag cagggtgcca agaaactgaa agtgaagatg
      601 gaccccagtc ttgtcgagaa acacattgag gcggtcaagt cccgatattc cagcgagaag
      661 gagtttcttc ggagcttgga gaaggaagga atgacgctcg aggcctatcg caacaatgtg
      721 accgatgaaa tcatcgttca aacagtcaaa gacagaatca tgggtgaggg cgttgtggag
      781 ccgacagatg aggaagtcga ggagttctac aacgcacatt tgaaggattt caagaagccg
      841 gagatgatcc atctgtgcct tctgatgatc gggtatcccg agaatcccgc catggaacag
      901 aagagcgaag ccttctcgat catcaatcgt ctttactttc ggattctggc cggggagaat
      961 tttgaggaac ttgccacaac ctattcgacc ggaccgaaca aggaaaaggg tggagacctg
     1021 ggatacaaac cgcgagagta tgtccagaag tacttggaga acgctcccga gtaccagttg
     1081 gctgctggca atgtaactcc cgtgcttgaa acgccaaccg gctatgtact gctctacccc
     1141 aaggagatcg ttcccaagaa ggatgtttcc ttggaacaag cttcccaaga gattcgagga
     1201 attctcaagg atcgccagaa agctcgaaac cttcagcgct ggctcaacag tcagacggaa
     1261 tcggcgaaga tggaagtcgt tgatccggat ctctataaac ttgtaagcgt ctcccaatag
     1321 ctgagtgctc ttggagcagt cgcccgaatg agaaaggatg agttgaagat gcccgcagta
     1381 gtagttgttg gtctgcaatg gggtgacgaa ggaaaaggca agatcgtgga tttcttgagc
     1441 cgagaagctc agctggtcat tcgtcatcaa ggcgggaaca atgca
//
LOCUS       DAOQUC010001181         1484 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_118641_length_1484_cov_2.646606, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001181 DAOQUC010000000
VERSION     DAOQUC010001181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1484
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118641_length_1484_cov_2.646606"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(78..515)
                     /locus_tag="PKH07_16695"
     CDS             complement(78..515)
                     /locus_tag="PKH07_16695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36628.1"
                     /translation="MNLRHVGLFLHGLVTQINATDQAEQAMGRAVLLTLLLLLLLGSL
                     VVLVYFWIRSHRRHSALADLNKELVSFEAMRRSGLLSEEEHRKIASRLREQIALQTVQ
                     QTVKEGETRPSDAMLIEILAAEASKLEARKKTGGASPGQKTEE"
     gene            complement(531..965)
                     /locus_tag="PKH07_16700"
     CDS             complement(531..965)
                     /locus_tag="PKH07_16700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36629.1"
                     /translation="MNGISFWETVDPSTLWHGVLFLILCVCAYWGVQKLLVRAENRRQ
                     DMAFYALVDKIGLDAEEETRLVDLLEKQKIGRPARVLESLKEYDLVAERAISAILTAE
                     EVWPERLKAIDKIYSARSKVDQFLLLAEHREMAPLKPPLKLE"
     gene            complement(965..>1484)
                     /locus_tag="PKH07_16705"
     CDS             complement(965..>1484)
                     /locus_tag="PKH07_16705"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNT36630.1"
                     /translation="MKIKNPAVQGQIAQKIYDGLKAYFGVETVEPTRSKPVASAASTT
                     SLRYHKTVKGDSLWSVAQTYGVNVEDLCNTNVIPGRVLREGQVLVIPGGSGFRIASDS
                     EPIASSAPAPTTRPSVLSEQSYSVKPGDNLYSISKRYSVSIQQLRTRNNLSKRSVIHP
                     GDVLVIPERPNR"
BASE COUNT          336 a          432 c          360 g          356 t
ORIGIN      
        1 caagcgtgtt ggcagtcccc aaatcgatcc ccatatcccg ggagatcagt cggtaaagat
       61 atcggaagat cattcagtca ctcctcagtc ttctgaccag ggcttgcccc gcccgtcttc
      121 tttcgggctt ccagtttgga agcttccgct gccaggattt cgatcagcat cgcatcgctc
      181 gggcgcgttt cgccttcctt cacggtttgt tggacggtct gcaaggcaat ttgttcacgc
      241 agacgactgg caattttgcg atgctcttct tcagacaaaa gaccactgcg tctcatcgcc
      301 tcaaaagaga ccagttcttt attcaaatcc gccaaagcac tatgcctgcg atggctccga
      361 atccaaaaat acaccaagac aacaagactc cccaacagca acagcagcaa cagggtcaga
      421 aggactgccc tgcccattgc ctgctcggct tggtccgtcg cgttgatttg tgtgacgaga
      481 ccatgcaaga acagaccaac gtgcctcaga ttcactgttg tgctcccatt tcactcaagt
      541 ttgagaggag gcttcaaggg tgccatctct cgatgttccg ccaacaacag aaactgatcc
      601 actttgcttc gggcggagta aatcttatct atcgctttga gcctctcagg ccacacttct
      661 tccgcggtca ggatggctga aatcgcccgc tcagcgacaa ggtcatactc cttcaatgac
      721 tcgaggactc ttgccggtcg gccgatcttc tgtttctcca gcagatccac gagtctcgtt
      781 tcctcttccg cgtcgagtcc gattttgtca acaagcgcat agaaagccat atcctgtcgt
      841 ctgttctcag cacgaaccag cagcttctga actccccaat aggcacacac acacaggatg
      901 aggaaaagca ccccatgcca tagcgtcgaa ggatccaccg tctcccaaaa ggaaatacca
      961 ttcatcagcg gtttggcctc tcaggtatga ccaatacatc cccgggatga atcacacttc
     1021 gcttgctcag gttgttccgg gtgcgtagtt gttggatgga gacactgtag cgcttcgaga
     1081 tggaataaag gttgtctccg ggtttgactg aataactttg ctcagacaga accgaaggac
     1141 gtgtcgtggg cgcgggtgcg gagctggcaa tgggttcaga gtcagacgca attcggaaac
     1201 cacttcctcc aggaatgacc agcacctggc cttcccgcaa gactctgccc gggatcacat
     1261 tggtgttgca gagatcttca acgttcactc cgtaagtctg ggcaacgctc caaaggctgt
     1321 cgcccttcac cgttttgtgg tagcgaaggc tcgtggtgga tgcggcggac gcgaccggtt
     1381 tcgaacgggt cggctccacg gtctcaaccc caaaatacgc cttcagaccg tcgtaaatct
     1441 tctgtgcaat ctgtccctgc acagcggggt tcttaatctt catt
//
LOCUS       DAOQUC010001182         1481 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_119002_length_1481_cov_3.893408, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001182 DAOQUC010000000
VERSION     DAOQUC010001182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1481
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119002_length_1481_cov_3.893408"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1189
                     /locus_tag="PKH07_16710"
     CDS             <1..1189
                     /locus_tag="PKH07_16710"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNT36631.1"
                     /translation="DLRGANDELIQHRRRLKEEVYQTNRLLQQRVVELSTLYEIGKTL
                     VAELDLPRLLKQTVERVSSLLGAVKTSLMIYDKDTRHLHIEVAHGFDEGDFPLAVPLG
                     TEPSGYCALTRQPLLVENIDLDGRFRGSIAPSYLGRSFICVPLVARNELIGVLNIADK
                     TNGQSFIQQELDFVVTLASQAAIAIDNATLYDHVQKAYFNTVQSLMGALEKKDRYTMG
                     HSERVTRYCKILADHMGVKTIDSQMMVLASSLHDIGKIGISESILCKPGALTDEEYMI
                     IKQHPVMGEQILRPLDFLGEGIQMVRHHHEREDGAGYPDGIAKQDLTLPMRILIAADA
                     FDAMTSIRPYRRSLSLNQAISELQKHKGSQFDPSVSDCFVDLLHEKADEIVPECKMEQ
                     VVN"
     gene            1186..>1481
                     /locus_tag="PKH07_16715"
     CDS             1186..>1481
                     /locus_tag="PKH07_16715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNT36632.1"
                     /translation="MKSQVEYINDIAVIRFAGELTIHNAHKVRALIEGEIIKGYRKII
                     IELEQVTYMDSTALGMLVAIYKTIRNDNQKLFIAGVNENLRRLLTLTRLNTFFVV"
BASE COUNT          357 a          361 c          402 g          361 t
ORIGIN      
        1 ggatttgcgt ggcgccaacg atgagcttat acagcatcgg agacgtttga aggaagaagt
       61 ctaccaaacc aaccggctgc tccagcagag ggttgtcgaa ctgtccacat tgtacgaaat
      121 cggaaagacg cttgtggcgg agttggactt gcctcgcctg ctgaaacaga ccgtcgagcg
      181 ggtctcctct ttgcttgggg cggtcaagac gtctttgatg atctacgaca aagacacccg
      241 ccacctgcat attgaagtcg cgcacggctt tgatgagggt gatttcccac tggccgttcc
      301 tcttggaacc gagccgtccg gatactgcgc cttgactcga cagcctctgt tggtcgaaaa
      361 cattgacctt gatggccgtt ttcgaggttc cattgcgccc tcatacttgg ggcggtcctt
      421 tatctgtgtt cctctggtgg cgcgcaatga actgatcggt gttctgaaca ttgccgacaa
      481 gaccaacggg cagtctttta tccaacagga actggatttc gtagtgactc tggcctcgca
      541 ggcagccatc gccattgaca atgcgaccct ctatgaccac gtccagaaag cctatttcaa
      601 cacggttcag tccctcatgg gagcacttga gaaaaaagac cgttacacca tgggtcactc
      661 ggaacgtgtg acacgctact gcaagattct tgcagaccac atgggtgtca agaccattga
      721 cagtcagatg atggtgttgg catcgagtct gcacgatatt ggaaagatcg gcatcagtga
      781 gtccattctg tgcaagccgg gagcactgac tgatgaggag tacatgatca tcaagcagca
      841 tcccgtgatg ggtgagcaga ttttgagacc gttggacttt cttggagaag gcattcagat
      901 ggttcggcat caccatgagc gggaagacgg tgccggatat cccgatggca tcgccaaaca
      961 ggacttgact ctgccaatgc ggattttgat cgcggccgat gcttttgacg ccatgacgtc
     1021 aattcgtccc taccgtagga gtctgtcact gaatcaggca atttctgagt tgcagaaaca
     1081 caagggttcc cagtttgatc cctcagtgtc ggattgtttt gttgatcttc tgcatgaaaa
     1141 ggcggacgaa atcgtccccg aatgcaagat ggagcaggtg gtaaattgaa gtctcaagta
     1201 gaatacatca atgatatcgc agtcattcgg tttgcgggcg agttgaccat ccataatgcg
     1261 cacaaagtgc gtgctctgat cgaaggggag atcatcaagg gctatcggaa gattatcatc
     1321 gagttggagc aggtgaccta catggacagc accgccttgg gcatgctggt tgctatctac
     1381 aaaacgattc gaaacgacaa tcagaagttg ttcattgccg gagtgaacga aaacctgagg
     1441 cggctcttga ctctgacccg tttgaacacg ttctttgtcg t
//
LOCUS       DAOQUC010001183         1481 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_119021_length_1481_cov_2.981066, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001183 DAOQUC010000000
VERSION     DAOQUC010001183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1481
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119021_length_1481_cov_2.981066"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1481
                     /locus_tag="PKH07_16720"
     CDS             <1..>1481
                     /locus_tag="PKH07_16720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36633.1"
                     /translation="LPEDVADLNENGNTSELLPYDLNGFRRLVQDSATNDLGVGTAPF
                     VDAGAHEFQLDCNGNDHPDYLDIADSTSNDANLNEIPDECELTARPVVYVDINATGNN
                     TGASWTDAFIDLQSALIFASTNGKGVVEQVWVANGTYYPDRGTGQRMRSFALPENVAL
                     FGGFAGADSVEHPGGEVSLSQRNMDANKTILSGDIGASGVWGDNSYHVVISDSDTDSP
                     AIDGFTITKGNADGADDSGKGGGLLVLGGSLKVWNCSFQDCRAKQGAGIYSHLGEPQL
                     VNCVIRNCQASEEGGALLAYGETVFPLINCTIVYNSAANSGGVLVTGNSSVYLMNSIL
                     WANTAGSGTTEQAQVAVVAGQVSLDSCCVQGYTGTLPGNLSFGLNPRFHNDGVSLQMD
                     SPCVDAGSSYALPEDLYDLDDDGDTSEFLPVDIKGYRRLLDSPHAPNVGLGDPVVDIG
                     AAEAWFDCDGNGIPDYIDTEKNPERDSDGDGVLDDCEYMAFVD"
BASE COUNT          332 a          372 c          433 g          344 t
ORIGIN      
        1 tgttgcctga ggacgtcgct gatctgaatg agaatggaaa tacctcggaa ctgcttccct
       61 atgatctgaa tgggttccga cggcttgtcc aggattccgc caccaacgat ctgggcgttg
      121 gaacagctcc gtttgttgac gctggcgctc acgaattcca attggactgc aacgggaacg
      181 accatcctga ctacctggac atcgctgaca gcacaagcaa cgatgcgaat ctgaacgaga
      241 ttccagacga gtgcgagctt accgcccgac cggttgtcta tgttgacatc aacgcaacgg
      301 gcaacaacac aggcgccagt tggaccgacg cctttatcga tcttcagtcg gcgcttatct
      361 ttgcctccac aaacggcaag ggtgttgtag agcaggtctg ggtggccaat ggaacctact
      421 atcctgatcg aggtactggc caacgaatgc gcagctttgc gcttccagag aacgtggccc
      481 tgtttggtgg ttttgcgggc gcggacagcg tggaacaccc tggcggtgag gtcagcctgt
      541 ctcagagaaa catggatgcc aacaagacca ttcttagcgg tgacattgga gcctcgggtg
      601 tttggggcga caacagctat cacgtcgtta tatctgactc ggacacggac tctcccgcga
      661 ttgatggatt caccataacc aagggcaatg ccgatggcgc tgatgatagt ggcaaaggtg
      721 gcggcctgtt ggttctcggt gggagtctca aggtttggaa ctgctcattc caggattgtc
      781 gagccaaaca aggtgctgga atctacagtc acttgggaga accgcaactg gtcaactgcg
      841 tcatcagaaa ctgtcaggca agcgaagaag gcggagcctt gcttgcctat ggggaaactg
      901 tatttcctct gatcaactgc accattgtct acaattctgc agccaactct ggtggtgtac
      961 tcgtcactgg taactcttcc gtctacctga tgaatagcat cctttgggcg aacacggcag
     1021 gttccggtac caccgaacaa gcgcaggtgg cagtcgtggc gggacaggtg agcctggaca
     1081 gttgctgcgt gcagggttac actggaaccc tccccggcaa tctcagtttc ggactcaatc
     1141 ctcgattcca caacgatggg gtgtctctgc agatggactc tccgtgtgtg gatgcgggaa
     1201 gcagctacgc gcttcctgaa gacttgtatg atcttgatga tgatggcgac acaagcgagt
     1261 ttctgcccgt tgatatcaag ggataccggc gtctgttgga cagcccgcat gcgccgaatg
     1321 tcggattggg cgatcccgtt gttgatatcg gtgcggctga agcatggttt gattgcgatg
     1381 gaaacggcat acctgattac atagacaccg agaagaatcc ggagcgtgac tcggatggag
     1441 acggcgttct ggatgactgc gagtatatgg cttttgtgga t
//
LOCUS       DAOQUC010001184         1481 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_119031_length_1481_cov_2.772090, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001184 DAOQUC010000000
VERSION     DAOQUC010001184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1481
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119031_length_1481_cov_2.772090"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1481
                     /gene="rpoB"
                     /locus_tag="PKH07_16725"
     CDS             <1..>1481
                     /gene="rpoB"
                     /locus_tag="PKH07_16725"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF016448.2"
                     /note="DNA-dependent RNA polymerase catalyzes the
                     transcription of DNA into RNA using the four
                     ribonucleoside triphosphates as substrates; beta subunit
                     is part of the catalytic core which binds with a sigma
                     factor to produce the holoenzyme; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HNT36634.1"
                     /translation="LVQASIIPNRGAWLEFENDLKNIHFVLIDRKRKIPVTCLLRAFG
                     LSTDEQIMGCFAQAEQVNLTKRFSKKNLVGKMAGQTVADQTTGKVICERGDELSDDQV
                     EELKDSGNENILIVDGDESRLTFLKETLTKDPSHDQESALLEIYQHLRPGEPPVLKNA
                     QALVENLFSRSRRYDLSRVGRYRMNKKLGLDVSLDEVTLSLADLIGVVQYMLNLEEGR
                     GLADDIDHLGNRRVRTIGELLEGQIRIGLARMERSIREKMGILDLENIMPRNLINAKP
                     ITAAINEFFGRSQLSQFLDQINPLAELTHKRRLSALGPGGLNRERAGFEVRDVHHTHY
                     GRICPIETPEGPNIGLIFSLATFGQINPFGFIETPYRKVEKGKVAESVEFLTADDEDE
                     CIIAQANTPVEGNRLAEDTVLCRHRGEFPLMPPTKVDYMDISPMQLVSVSAALIPFLE
                     HDDANRALMGSNMQRQAVPLLKTEAPLIATGVEHKAAKDSGVV"
BASE COUNT          363 a          398 c          389 g          331 t
ORIGIN      
        1 ccttgtccaa gcaagcatta taccgaaccg gggagcttgg ctggagtttg agaacgacct
       61 gaagaacata cattttgtgc tcattgaccg aaagcgcaaa atccctgtga cctgccttct
      121 gagggcattt ggactgtcca ccgatgaaca aatcatgggc tgtttcgctc aagcagaaca
      181 ggtcaacctc acaaagcgtt tttcgaaaaa gaaccttgtg ggcaagatgg cgggtcagac
      241 ggttgccgac cagaccacag gcaaggtaat ctgtgagcgc ggtgatgaac tgtccgacga
      301 tcaggttgag gaactgaagg actcaggtaa tgagaacata ctcattgttg acggagatga
      361 gtcccgcctc accttcctga aggaaacgct caccaaggat ccctcacatg atcaggagag
      421 cgcactcctt gagatttacc agcatctccg accgggagag cctcccgtac tgaagaatgc
      481 acaggccttg gtggagaatc tgttctcccg cagccgccgg tatgatctct ctcgtgttgg
      541 tcgctaccgg atgaacaaga aactcggcct ggatgtttct ctggacgaag taaccttgtc
      601 cctggccgat cttattggtg tcgttcagta catgctcaac ctggaagagg gcagaggctt
      661 ggctgacgat attgaccatc tcggcaatcg tcgtgtgcgt accatcggtg aacttctgga
      721 agggcagatc cgcattggcc ttgcccgaat ggagcgatcc atcagagaga agatgggcat
      781 tcttgacctt gaaaacatca tgcccaggaa tctgatcaat gccaagccaa tcacagccgc
      841 catcaatgaa ttcttcgggc ggagtcagct ttcacaattc ctcgaccaga ttaaccctct
      901 ggccgaactg acccacaagc gccgactctc cgctttgggc cccggaggct tgaatagaga
      961 acgcgcgggc tttgaagttc gcgatgttca ccatactcac tacgggcgca tctgtcccat
     1021 tgagacacct gaaggtccaa acattggcct tatcttctct ctggcgacat ttggacagat
     1081 caacccattc gggttcatcg aaactcccta tcgaaaagtc gagaaaggta aagtggccga
     1141 gtcggttgag tttctgactg ccgacgacga agacgagtgc attatagccc aggccaacac
     1201 tcccgttgag gggaatcgcc tggccgagga tacggtgctt tgccgacatc gaggtgagtt
     1261 tccgttgatg ccacccacca aagtggacta tatggacatt tccccgatgc aattggtgtc
     1321 tgtttccgcc gcactgatcc cgtttctgga acatgacgat gccaaccgcg cactgatggg
     1381 aagcaacatg caacgtcagg cggtgcctct gctgaagacc gaagccccct tgattgctac
     1441 tggtgttgaa cacaaggccg caaaagactc cggtgtcgtt c
//
LOCUS       DAOQUC010001185         1480 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_119148_length_1480_cov_3.429474, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001185 DAOQUC010000000
VERSION     DAOQUC010001185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1480
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119148_length_1480_cov_3.429474"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            320..580
                     /locus_tag="PKH07_16730"
     CDS             320..580
                     /locus_tag="PKH07_16730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36635.1"
                     /translation="MTTLKASQARIPTEVFNRVVYKGEAIRVEHRSAGALFLISEDEY
                     KAFRDWEDMMDLKAFRKAKKEFLESGEKAIPWSQAKKELGLE"
     gene            577..837
                     /locus_tag="PKH07_16735"
     CDS             577..837
                     /locus_tag="PKH07_16735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HNT36636.1"
                     /translation="MTRYSISITASAQRSIARLEKPIQRRIQQAIDRLEETPRPFGAE
                     KMQGEPNGYRLRVGDYRILYEIGDRELLVLVVKVGHRREVYR"
BASE COUNT          371 a          341 c          416 g          352 t
ORIGIN      
        1 ttccatgtga agttcaagga acccggcacg gtgatgatga ccagcccata cacaggtcag
       61 acctacggat ttggtggtga gaacggctgg ctgtcacccg gaaccggcat cttgctcagt
      121 ctagggaaat aggcgaatgc ctctcagcac cgagtgctgt ctgtaacttg gctgtgcttc
      181 ccgtatccct gccagtcgcc tgaacgagat tctgctgggt gggctggcag cgtctttttc
      241 agttttccag ttgacataag gtaacaccct tcttattgta caattcgtac aattggatca
      301 attgaaagag gtgatggcaa tgacaacact gaaggcgtct caggcgcgaa ttccgacgga
      361 agtcttcaac cgtgttgttt acaagggtga ggcgattcgc gttgaacacc gaagcgccgg
      421 agccttgttc ctgatctccg aagacgagta caaggcgttt cgtgattggg aagacatgat
      481 ggatctgaag gctttccgaa aagcaaagaa ggaattcctc gaaagcggcg agaaggcaat
      541 accgtggagt caggcaaaga aagagctcgg tcttgaatga cacgctatag tatttctatt
      601 acagcctccg cacaaagatc tattgcccgt ctcgagaagc caatccaacg ccgaatacaa
      661 caggcaatag atagactgga agagactcct cgtccctttg gtgcagagaa gatgcaggga
      721 gagccaaacg gctaccgcct tcgcgtcggt gattaccgca ttctgtatga gattggggat
      781 cgggagctct tggtgcttgt tgtcaaggtt ggacatcgaa gggaagtata tcgttgatca
      841 gtccctgtct ttcagaagca gcagccacct cttgctcagt ctagggaaat gggcgaatcc
      901 gagattcccg atggtcaacg ttcacagtgt gttgcggggg acactttcgt ctttcttgtc
      961 tcctagtgat actcagtcca aggaaacgca tcatccgccg agtaggcatc caaggggggg
     1021 gcattgccca cacgcccgca ggtgctgtcc tgctcgaaag tgtgccggag ctgacacaat
     1081 ttcacgagtc tgttacaaaa gaaatgcatc atgggatgcc cagttcggtt gagtccgatt
     1141 tggctctcta gtgcttttga ggagtcaggg tctggcgtta gctcatgaag attgagattt
     1201 catagacagt atatgaagac aaggccaccc aggagtcctc agaaactgca taagaaccgc
     1261 agggtgagcg tgttttcgct cagtctttga gcctgaaagg caaattcctt ccggctcagt
     1321 ggaaagcgtc atcggaattc tgttgagatg gcactacata tccaggactt ccgtagtgaa
     1381 gtgcctgaaa aggaggattt tatcgtggca ggtatcgctg gaatagctga atccggaagg
     1441 cgacgcgaag ttgggcagat ggttgaaaga ctgagtcaca
//
LOCUS       DAOQUC010001186         1480 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_119157_length_1480_cov_3.164211, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001186 DAOQUC010000000
VERSION     DAOQUC010001186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1480
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119157_length_1480_cov_3.164211"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            19..>1480
                     /locus_tag="PKH07_16740"
     CDS             19..>1480
                     /locus_tag="PKH07_16740"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNT36637.1"
                     /translation="MRVLNRVNLLCAALLAGIAYLGAVFCLAQDTSPSVLDQFKTHYL
                     NGEYDQAEALLKQTADESLKNGNQKDASDLLYRLGFLYDRVGKFDQAMGAYGDSQQLA
                     RIVGDQSLVVKVAFNSAATSLSLGDAKRALAIYQGLLEAFEAAGAEEAKARCLQNRGA
                     VLMQQGELSPAYKDLTEALKIYESKNSQGRIAETVTNLGILLTREGLYDQAKDQFERA
                     AAIFTASSDQRSLATVLINQAELAQKQGDAATALQLLDKALQAKKATQDRMGEAAALQ
                     VIAQTLYSQGEHQKALDTLGQSLALRVELKDVLGEGSCHLLMGEWLFRIGGAENFQKA
                     EVHLSRAYDAFKNAGYKQYLQRVLYWQGRISDARGNVEQAEKKFNEAITELESLTYRL
                     PDDVQATKVVSTERSEPYEALVSLLIRQNRLPEALAYLDRSRDQNLAKDLIGGTSEEI
                     SYQSRIAALRERIEQAKSEKDTKLAEMLAKEAEGVQD"
BASE COUNT          364 a          374 c          432 g          310 t
ORIGIN      
        1 atgcgcagga gagccttagt gcgtgtcttg aatcgagtga atcttctctg tgctgcactc
       61 cttgctggta ttgcctatct gggagcggtc ttttgcctgg ctcaggacac gtctccatca
      121 gtcctggatc agttcaagac gcattacctg aatggtgaat acgatcaggc tgaagccctg
      181 ctgaaacaga cagcggatga gagcctcaag aacggtaacc agaaggatgc ctctgatctt
      241 ctctatcgtt tggggtttct ctatgaccgc gttggaaaat tcgatcaagc catgggagcc
      301 tatggtgatt cgcagcagtt ggcccgaatt gtcggcgacc agtcgctggt ggtcaaggtg
      361 gcgttcaaca gcgctgcgac gtcgctaagt ctgggggatg ccaaacgcgc tcttgccatc
      421 taccaaggtc ttctggaagc ctttgaggcg gcaggtgccg aagaagccaa ggcgcgctgt
      481 cttcagaatc gtggcgctgt gctgatgcag caaggtgaat tgtcccccgc ctacaaagat
      541 ctcaccgagg cactgaaaat ctacgagagc aaaaacagcc agggacgcat cgccgaaacg
      601 gtgactaatc tgggtattct cctgactcgg gaaggtttgt acgatcaagc caaggatcag
      661 tttgaacgtg ccgctgcaat cttcaccgct tcgagcgatc agcgctcttt ggcgacggtg
      721 ttgatcaacc aggcggaact ggcacagaaa caaggtgacg ccgccactgc gctgcaactg
      781 ctcgacaagg cactccaggc caagaaggcc acacaggatc gcatgggcga agccgctgcg
      841 cttcaggtca ttgcacagac cctgtacagt caaggagagc atcagaaggc actggacaca
      901 ctgggacagt ctcttgctct aagagttgag ttgaaggacg ttctgggaga aggttcctgt
      961 catctgctga tgggagagtg gctgttccga atcggtgggg cggagaattt tcagaaggcg
     1021 gaagtgcatt tgagtcgtgc ctatgatgcc ttcaagaatg ccggctacaa gcaatatctt
     1081 cagagagtcc tttactggca gggccggatt tcggacgcgc gaggcaacgt cgagcaagct
     1141 gagaagaagt tcaacgaagc catcacggaa ctggagtcgc tgacgtatcg gctgcctgac
     1201 gatgtccagg caacgaaagt tgtcagcacc gagcgctcag aaccttatga ggcgttggtc
     1261 agtctgttga ttcggcagaa ccgtctgcct gaagcccttg cctatctgga tcgcagccgc
     1321 gatcagaatc tcgccaagga tctcatcggc ggaaccagcg aagagatttc atatcaaagc
     1381 cgaatcgccg ctttgcgtga acgaatcgaa caagccaaat cggaaaaaga taccaaactg
     1441 gcagaaatgc tggccaagga agccgaaggg gtacaggacg
//
LOCUS       DAOQUC010001187         1479 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_119309_length_1479_cov_2.703652, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001187 DAOQUC010000000
VERSION     DAOQUC010001187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1479)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1479)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1479
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119309_length_1479_cov_2.703652"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..947
                     /locus_tag="PKH07_16745"
     CDS             <1..947
                     /locus_tag="PKH07_16745"
                     /inference="COORDINATES: protein motif:HMM:NF024045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HNT36638.1"
                     /translation="GVIGKAVQEGSNVLVQLEAAIDFPTEELDADASNTLTEKLQDAF
                     SSLSDLLSQARIRVNQLEGLRTPICGKRNVGKSSLLNRLLGRDRALVTPYPGTTRDTV
                     EEDTVLEGVLLHLSDTAGIGETEDPVELLGLQRTNEAIQDADIILFIVDISEPISRED
                     HQVFALIKEHFPKHLDERVLLLANKVDRLRDSDTRDAVLDSLRREFEPLSPLSISALT
                     GEGINTLCQRLAGIAHHEQSEVERSAAGARVRQRILLHEAVEAITRAIPLTHPPQDNP
                     ELAVIEIRTALNALEQLDGVNVCPDVLESIFSRFCIGK"
     gene            complement(984..>1479)
                     /locus_tag="PKH07_16750"
     CDS             complement(984..>1479)
                     /locus_tag="PKH07_16750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36639.1"
                     /translation="QWSFKALEQATSTPTETPTATPTFTQVPGYSVNIQSTTPSISLP
                     LTVGETYTISALAQCLVDNSGVGARFQASLWNNSGQFRVNPAQFLPSASTQQDLSTGF
                     SSVFVENNGPVVLKARIWDNELTTLLGEDTETFQVVTPTPTLIPTVTPTSPPSRVERW
                     ATYE"
BASE COUNT          340 a          401 c          405 g          333 t
ORIGIN      
        1 tcggtgttat cgggaaggct gttcaggaag gatcgaatgt cttggttcaa ctggaggcag
       61 ccattgattt cccaacagaa gaactggacg cagatgcctc aaacacactg accgaaaaac
      121 ttcaggacgc tttttcttcg ctctcggatc tgttgtcgca agccaggatt cgagtcaacc
      181 aacttgaggg acttcgaacg cccatttgcg gaaagcgcaa tgtgggtaag tcaagtttgc
      241 tcaatcggct cctgggacgc gaccgagccc tggtcacgcc ctatccagga acgacccgag
      301 acacggttga agaagacacc gtgcttgaag gagtactcct tcatctgagt gatacggcgg
      361 gcatcggtga gaccgaagat cctgtcgaac tcctgggact tcaacggacg aacgaagcca
      421 tccaggacgc ggatatcatc ttgttcatcg tggacatctc cgaaccgatc agccgcgaag
      481 atcatcaggt ctttgcgttg atcaaggagc atttccccaa gcatctcgat gaacgggttc
      541 ttctgttggc caacaaagtg gatcgcctca gagactcaga cacacgcgat gcggttctgg
      601 acagtctccg acgggagttt gaacctttgt cgccgctttc aatctctgcg cttacaggtg
      661 agggcatcaa cacgctgtgc cagagacttg cgggcatcgc gcaccatgag cagtccgagg
      721 tggaacgctc ggccgctggc gctcgtgttc gccagcggat tcttctgcat gaagcggtcg
      781 aggcgatcac gcgagccatt cccctcacgc acccgccaca ggacaacccg gaactggctg
      841 tcatcgaaat ccgaacggca ctgaacgctc tcgaacagct cgatggcgtc aacgtctgcc
      901 ccgacgtgct tgaatccatc ttctcgcgat tctgcatcgg gaagtagatg gacttcccaa
      961 gcacttatga gagaaaccca cacttattca taggtcgccc atctctcaac gcgtgagggt
     1021 ggagaagttg gggtcacagt tgggatcagt gtcggtgttg gagttacgac ctgaaacgtc
     1081 tccgtatcct ccccgagcag cgttgtgagt tcgttgtccc agattcgtgc cttcaaaaca
     1141 acaggaccat tgttttcaac gaacacgctt gagaaaccag tgctcaaatc ctgttgggta
     1201 gaagcgctgg gcagaaactg tgccggattc accctgaact gacctgagtt gttccacaat
     1261 gatgcctgga acctggcacc cactcccgag ttgtcaacaa ggcactgtgc caatgcgcta
     1321 atcgtgtatg tttctccaac agtcaaaggc aaagagatcg agggtgttgt ggactggata
     1381 ttcaccgagt aaccgggcac ctgtgtgaac gttggagtag ctgtcggcgt ttcggtcggt
     1441 gtgctggtcg cctgctccag tgctttgaat gaccattgg
//
LOCUS       DAOQUC010001188         1477 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_119545_length_1477_cov_3.234880, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001188 DAOQUC010000000
VERSION     DAOQUC010001188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1477)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1477)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1477
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119545_length_1477_cov_3.234880"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            275..955
                     /locus_tag="PKH07_16755"
     CDS             275..955
                     /locus_tag="PKH07_16755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36640.1"
                     /translation="MSRPAIPDVVTIVGSAYLQPIADLLDKLLKRPVSGVGPAGTSLH
                     ENGYSAALVVLLIAVLESYTARLRFVRNVEGVAGNLSTPDLLAKYFTALPTRDELVDV
                     FLVRNVLAHNHVWHLDVSDFEAVGSPTLATPKELGFQTNKHYEQVVDVPARQTRTLRL
                     NVSPTAVDRSDVRKVFDVVWRTLKFMNQENFSHTPLASCTVGFRGKHRQFEDLLKEIT
                     DEGVSHAP"
     gene            1090..1329
                     /locus_tag="PKH07_16760"
     CDS             1090..1329
                     /locus_tag="PKH07_16760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018267773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4160 domain-containing protein"
                     /protein_id="HNT36641.1"
                     /translation="MSPTVFREGGFRFYFFSREEPRMHVHVQGQNGEAKFWLEPVIEV
                     AQHTGLSRREISEAQRLVQEHEYDIRSAWHRHFPG"
     gene            1283..>1477
                     /locus_tag="PKH07_16765"
     CDS             1283..>1477
                     /locus_tag="PKH07_16765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020565568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HNT36642.1"
                     /translation="MNMTSAALGTDISPVEVTNVSQHGFWVLLDAEELFLPFSEFPWF
                     RDVAIGKILHVELPAPNHLYW"
BASE COUNT          303 a          398 c          401 g          375 t
ORIGIN      
        1 ggagcccgcg gatttttgtg acgccttcag gtcgattatg agtggatagt tttttgggag
       61 agcgcgatgg gtgaatggtc tgaatacttt gaagattttt ctgaggaaaa tacggcgaat
      121 tatgttggcg gacgtttcga ttcaaagcac tgttcccgcc ctgttgaaaa tcgtgctgct
      181 gaaaaccgtg gtctgtcccc gttttccgcg ttttccgcgt ttgtccccgt tttccgcgtt
      241 tcgcccagat aatccttagg gcgcaggagc agccatgtcc cgaccagcaa ttcccgatgt
      301 cgtcaccatc gtgggctctg cttatcttca gccgatcgca gatctactgg acaagcttct
      361 gaagcgacct gtatccggcg tgggtcccgc tggaacaagt ttgcatgaga acgggtattc
      421 ggctgctctc gttgtccttc tgatcgctgt gctcgagtcg tataccgcaa ggcttcgttt
      481 tgtgcggaat gtggaaggag ttgccggaaa cctcagcacg cctgaccttc tcgccaaata
      541 ctttacggct ctcccgacca gggatgaact tgtcgacgtt ttcctggttc gtaatgtgtt
      601 ggcccacaat catgtctggc acttggacgt ctcagacttc gaagccgttg gatctccgac
      661 tcttgctact ccaaaagagc tcggctttca gacaaacaaa cactacgagc aggtggttga
      721 cgtccccgct cgtcagactc gtaccttgag gctgaatgtc agtcccaccg ccgttgaccg
      781 atccgacgta cgcaaggtct ttgatgtggt gtggcgcacg ctcaagttta tgaaccagga
      841 aaacttcagc cacacaccac tcgccagctg tacagtgggc tttcgcggta agcaccggca
      901 gttcgaggac ctgctgaagg agatcacaga cgagggtgtc agtcatgcgc cctaaccaat
      961 catttcaccg gacctgtcgc gcacaaaata ggggtcagac ccctattttt tcctctgagc
     1021 gaaaaagccg cgcagcgccg gtgatttcta cgttgggcgg cgacaagctc tacgcgtatc
     1081 attccaccga tgagtccaac tgtttttcgt gaaggtggct tcaggttcta tttcttctcc
     1141 cgagaggagc cgaggatgca tgtgcacgtt cagggacaga acggtgaggc aaagttctgg
     1201 ctcgaacctg tgatcgaggt cgcacaacac accggcttgt ctcggcggga aatcagcgag
     1261 gcacaacgcc tcgtgcagga gcatgaatat gacatccgca gcgcttggca ccgacatttc
     1321 cccggttgaa gtaaccaatg tttcgcagca cggcttctgg gtcttgctgg acgcggagga
     1381 gttgttttta cccttctccg agtttccgtg gtttcgggat gtggcgattg gcaaaatcct
     1441 ccatgtcgaa ctgccggcgc ccaatcatct ctattgg
//
LOCUS       DAOQUC010001189         1476 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_119709_length_1476_cov_2.935961, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001189 DAOQUC010000000
VERSION     DAOQUC010001189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1476
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119709_length_1476_cov_2.935961"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1476)
                     /locus_tag="PKH07_16770"
     CDS             complement(<1..>1476)
                     /locus_tag="PKH07_16770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36643.1"
                     /translation="KRAQNQFLQAETLALLTILVVVLLVSLSKIDDYDIWFHTKSGEY
                     YWNTRSLPQTDPFLFGMDEGVRDVSEWLSQLLMFFIWSAGGGVALQLANSLVLIATFL
                     LLYKLIRLRECEQWLALAMTLIAIFVARRRWMPRPFQLVNLGIVILFWVVERWNREGR
                     NLPRAWVFALWFMLWTNLHPGAYSGLALLVLFWVSSQFSRKKMPLSVPLASGIGSLLN
                     VDLPGQLHKVLTCMTGGETIQNVLEHMPADMDWISGWFGVFALIVLATLPRIVGKRRW
                     LDALLVAGILLYSFRSRRYIDLLAIIASPPVAQCFSEDLKRIRWNAPMRFRKPAIGLL
                     IGTILAGGIFSMGIDRSYRAGFGVYGEQLPAEAVAKAQEFGFSGNCFCTYPWGGYLSF
                     YNYPKWKVYIDGRCLMYGSKRLNEYFDILRAENWQNIFDKYDVHFAVIDSVESGAGEG
                     DIKLPNIAGALFESPDWALVYWDDLSRLFVKRGCGYDPIIA"
BASE COUNT          401 a          417 c          371 g          287 t
ORIGIN      
        1 gcgcgatgat cgggtcatat ccgcaccccc tcttcacaaa aagccgactc agatcgtccc
       61 agtagaccaa cgcccagtcg ggggactcaa acagagcgcc tgcgatattg gggagtttga
      121 tgtccccctc tcctgcaccc gactccacgg aatcaatgac agcgaaatga acgtcatact
      181 tatcaaagat attctgccag ttctctgcac ggagaatgtc gaagtactca ttcaggcgtt
      241 tggaaccgta catcaggcaa cgaccgtcta tgtagacctt ccatttgggg tagttgtaga
      301 aactgagata ccctccccat ggataggtgc agaagcagtt cccggaaaag ccaaactcct
      361 gagccttggc aaccgcctct gctggcagtt gctcgccata cacaccgaaa ccggcgcgat
      421 agctccgatc tatgcccatc gagaagatac ctcctgcaag gatcgtcccg atcagcaatc
      481 caatggcagg ttttctgaat cgcatcggag cgttccagcg gatacgcttc aggtcttcac
      541 tgaagcactg tgccacgggg ggcgatgcaa tgatcgcgag aagatcaatg taacgtcggc
      601 tccgaaaaga atacaagaga atgcctgcga ccaggagcgc atcaagccat cgtctctttc
      661 cgacgatgcg gggcagcgtt gccaaaacga tgagagcaaa cactccaaac caaccgctga
      721 tccagtccat gtcggcaggc atgtgctcca gcacattctg gatcgtttcg cctcccgtca
      781 tacaggtgag aactttgtgc aactgtccag gcaagtccac gttcaacagg gaaccgatgc
      841 ccgaagccag aggaaccgaa agaggcattt tctttcgcga gaattggctg ctaacccaga
      901 acaggacaag cagcgccaaa cctgagtaag cgcccggatg caagttcgtc cagagcatga
      961 accagagcgc gaatacccaa gcacgtggaa ggttgcgtcc ttccctgttc catcgttcga
     1021 caacccagaa caggatgaca atgccgagat tcaccagttg aaagggtcgc ggcatccagc
     1081 gtcggcgcgc cacgaaaatt gcgatcagag tcatggcaag agcgagccat tgctcacact
     1141 cgcgaaggcg tatcaacttg taaagcaaca gaaacgtcgc gatgagaaca agtgaattcg
     1201 ccaactgaag ggcaacgcca cctccagcgc tccagatgaa gaacatcagc agctgagaga
     1261 gccattcgga gacatccctg actccttcgt ccattccaaa gaggaaagga tcagtctgag
     1321 gcaggcttcg ggtgttccag taatactcac cggactttgt atgaaaccag atgtcatagt
     1381 cgtctatctt gctcagactc acgagcaaaa cgacaacaag gatagtcaag agcgccagtg
     1441 tctcagcctg gagaaactga ttctgtgctc tcttcg
//
LOCUS       DAOQUC010001190         1472 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_120199_length_1472_cov_3.419195, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001190 DAOQUC010000000
VERSION     DAOQUC010001190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1472
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120199_length_1472_cov_3.419195"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(34..174)
                     /locus_tag="PKH07_16775"
                     /pseudo
     CDS             complement(34..174)
                     /locus_tag="PKH07_16775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328079.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
     gene            253..453
                     /gene="thiS"
                     /locus_tag="PKH07_16780"
     CDS             253..453
                     /gene="thiS"
                     /locus_tag="PKH07_16780"
                     /inference="COORDINATES: protein motif:HMM:TIGR01683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HNT36644.1"
                     /translation="MFLTVNGRKHEHQGNGTLLSLLGEIGAEAKRVAVVLNDEVVPRT
                     SFECTDLKPDDRVEILTFAGGG"
     gene            455..1246
                     /locus_tag="PKH07_16785"
     CDS             455..1246
                     /locus_tag="PKH07_16785"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011744613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HNT36645.1"
                     /translation="MSASKGLSLGTHTFESRLLLGTGKFSSVDIMIRAVKASEAQLVT
                     VALRRFNPQNLEDDLFTPLSQLDNVTLVPNTSGARDAKEAVRVAMLGRELSGSPFVKL
                     EIHPNPHHLMPDPIETYQAAQILVKEGFLVLPYISADPVLAKRLEDVGCAAVMPLGSA
                     IGSGQGLKTYEMIALIAQNSIVPVIVDAGLRAPSEAAAALEMGCDAVLVNSAIAAADD
                     PEQMAAAFAAAVRAGYQARHAGLMSRSDAAVATSPLTSFLSQEPQ"
     gene            1243..>1472
                     /locus_tag="PKH07_16790"
     CDS             1243..>1472
                     /locus_tag="PKH07_16790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36646.1"
                     /translation="MNDLLAVLESVESLTRLQSEADERSVEAALHCTHPDFRELAILL
                     SPAAGQHLEKMAQRAQALTQKHFGRTISLYVP"
BASE COUNT          320 a          402 c          413 g          337 t
ORIGIN      
        1 ctgtcaaggc tggcagtccg tcaagccctc cggcaaaccg gcaaccgaac ccaacccacc
       61 aacgccaacc atcagaacgc ttcccttgga gagtctttcc tgccccgact gtccgatctc
      121 tggcaacgcg gtctgtcgga catgacgcgt ttcgactgtg ttgctgcttt tcatttgaca
      181 acctcaggtc gccggatagt attcatagac gcgcatgtgc tgtgaaggca catagcagga
      241 gtatgataca gaatgttttt gacagtcaac ggaagaaaac atgagcatca gggcaacggg
      301 acgctgctct cgctgcttgg ggagatcggc gcggaagcca aacgtgttgc cgttgtgctg
      361 aacgatgaag tggttccaag aacctcattc gaatgtaccg atctcaagcc ggatgaccgc
      421 gttgagattc tcacttttgc gggaggcggc taggatgtcg gcaagtaaag gactttctct
      481 gggcacacat acttttgaat ccaggctgct cttgggaact gggaagtttt cgtctgtaga
      541 catcatgatt cgcgcggtca aggcgtccga ggcacaactg gttactgttg ccctgcggcg
      601 ctttaaccct cagaacctgg aagacgatct cttcacgcct ctttcgcagt tggacaatgt
      661 gacactcgtc cccaacactt ccggtgcgcg tgacgccaaa gaagccgttc gggtcgcgat
      721 gttggggaga gaactcagtg gcagcccatt tgtaaaactg gaaatccatc cgaaccctca
      781 tcatctgatg cctgacccga ttgagacata ccaagcggca cagattcttg tgaaagaggg
      841 gtttcttgtg cttccttaca tttccgcgga tcccgtcctg gccaagcgtc tggaagatgt
      901 tggctgtgcg gcggtcatgc ctctgggttc ggccatcgga agtggtcagg gtctgaagac
      961 gtacgagatg atcgctttga tcgctcaaaa cagcatcgtt cccgtcattg tggacgcggg
     1021 actgagagcg ccttcggaag ccgccgctgc gttggagatg ggttgtgacg ccgttttggt
     1081 caacagcgct atcgctgcgg cggatgaccc cgaacagatg gcagccgcat ttgcagctgc
     1141 cgttcgggcg ggataccagg cacggcacgc cggtctgatg agtcgaagcg atgctgctgt
     1201 cgcgaccagt cctctgactt ccttcctgtc acaggaacct caatgaacga tcttcttgct
     1261 gtgctcgaat ccgtggaaag tctcaccagg cttcagtctg aggcggatga gcgaagcgtt
     1321 gaggcagcac tccattgcac gcacccggat tttcgggaac tggccattct tctttcaccc
     1381 gcagcgggac aacatcttga gaagatggca caacgagcgc aggcgctcac acagaagcac
     1441 tttggtagaa cgatatcact ctatgtaccg tt
//
LOCUS       DAOQUC010001191         1471 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_120339_length_1471_cov_3.331921, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001191 DAOQUC010000000
VERSION     DAOQUC010001191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1471)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1471)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1471
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120339_length_1471_cov_3.331921"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /locus_tag="PKH07_16795"
     CDS             complement(<1..398)
                     /locus_tag="PKH07_16795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNT36647.1"
                     /translation="MKRIVAKEDVCLGCRLCEVHCVVQHSKSKRIVKAFREGKDTIVP
                     GVTVEESGSVSFAVQCRHCEEAPCLEACMTGGMHRDPQTGAVLNDPDKCVGCWMCIMV
                     CPVGAIQRGLDRKVASKCDLENLRQVSQQTA"
     gene            complement(404..>1471)
                     /locus_tag="PKH07_16800"
     CDS             complement(404..>1471)
                     /locus_tag="PKH07_16800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HNT36648.1"
                     /translation="EPSSDGEKSITEIPPQLEIQIPIMFAAMSYGSISYNASLALAQA
                     AAELGTFANTGEGGLHQDFRKFGKNLIVQVASGRFGVDVDYLNAGQAVEIKIGQGAKP
                     GIGGHLPGEKVDAAISETRMIPKGTDAISSAPHHDIYSIEDLRQLIYALKEATRYTKP
                     ISVKIAAVHNVAAIASGSVRAGADIVVIDGFRGGTGAAPTMIRNHVGIPIEFALASVD
                     SRLREEGIRNQASLVVSGGTRCSADIVKAIALGADAVNIGQAALVAMGCHMCQKCYTG
                     RCNWGIATQDPYLTKRLNPKVATRRLINLMRAWALEIKEMMGGMGVNAIESLRGNREH
                     LRGIGLTDRELNILGIKAAGE"
BASE COUNT          329 a          441 c          364 g          337 t
ORIGIN      
        1 gcggtctgct gggaaacttg acgcagattc tcgagatcgc attttgaggc aaccttgcga
       61 tccagtcctc gctgaattgc acctactgga caaaccatga tacacatcca gcatccgacg
      121 catttatcgg gatcattcag cactgctccg gtttgtggat ctcgatgcat tccgcctgtc
      181 atgcaagcct caaggcatgg tgcctcttca caatgccgac actgcactgc aaaggagacg
      241 gaaccggact cctcaacagt aacacctgga acaatggtgt ctttgccttc ccgaaatgct
      301 ttgacaatcc gcttggactt ggagtgctga acgacacaat ggacttcgca caaacgacag
      361 cccagacaga catcctcttt ggcaactatt ctcttcatcg aacctactct cctgcagcct
      421 tgatacccag gatgtttagt tccctgtccg tcagcccgat gccacgaagg tgctcacggt
      481 tgcccctcag actttcgatg gcgttgacac ccatgcctcc catcatctcc ttgatctcca
      541 gtgcccaggc acgcatgagg ttgatgagac gccgtgtagc tactttggga ttgagtctct
      601 tggtcagata gggatcctgc gttgcaatac cccagttgca tcgcccggtg tagcacttct
      661 ggcacatgtg acaacccatt gccaccaagg cggcctgtcc gatgttgacc gcatccgcac
      721 ccagggcaat cgctttgaca atgtcggcgc tgcaccgtgt gcccccactc acgaccagag
      781 aagcctggtt gcgtatccct tcttcacgca accgggaatc caccgaagcc aaggcgaact
      841 cgatggggat tccgacgtgg tttcggatca tcgtgggcgc cgctccggtg ccgcctcgaa
      901 aaccgtcaat gactacgata tctgcgccag cccgaacaga cccactggcg atggcagcaa
      961 cgttatgtac cgcagcaatc ttgacagaaa tcggtttcgt atatcgtgtc gcttctttca
     1021 aggcatagat cagttgccgc agatcctcga tggagtagat atcatgatgc ggcgcagatg
     1081 aaatggcatc ggtgcccttg ggaatcatac gggtctctga aatcgctgcg tccactttct
     1141 cgccaggcag atgaccacca atgcccggtt tggctccctg gccaatcttg atctcaaccg
     1201 cctgtcccgc gttgagatag tccacatcca caccaaaacg accggaagcc acctgaacaa
     1261 tcaggttttt gccgaacttg cgaaaatcct gatgcaagcc accttcgccg gtattggcga
     1321 acgtccccaa ctccgcggcc gcttgcgcca atgcaagaga ggcgttgtaa ctgatcgagc
     1381 cataggacat ggcggcaaac atgattggga tctggatttc caactgcggt ggaatctcgg
     1441 tgatggactt ttctccgtca gaggacggtt c
//
LOCUS       DAOQUC010001192         1471 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_120358_length_1471_cov_2.973870, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001192 DAOQUC010000000
VERSION     DAOQUC010001192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1471)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1471)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1471
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120358_length_1471_cov_2.973870"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..612)
                     /locus_tag="PKH07_16805"
     CDS             complement(<1..612)
                     /locus_tag="PKH07_16805"
                     /inference="COORDINATES: protein motif:HMM:NF015894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HNT36649.1"
                     /translation="MTAEEKKFKITVSPDRTQVLLDCTVEEGLLEDLVLEILEALQER
                     EIVQLPELEQLREAISQALGEDKRIKSLLVVKGQEPVAGNDGEIKWARDFFTTGFVFD
                     ERTGAIDYRQRKDSSEVQENELLCRVIPPLDGQPGTDVFGKLIPFKKGKPAKVFPGPN
                     VRRESEGDISNFYSESSGRVRWLGEKLSVDPVYVIQGSVGLETG"
     gene            complement(671..1201)
                     /locus_tag="PKH07_16810"
     CDS             complement(671..1201)
                     /locus_tag="PKH07_16810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012681851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HNT36650.1"
                     /translation="MALSKSVNQEQSGQEMGMSDFAGMYLTFQLGGETYGLEILKVQE
                     IIGMMNVTRVPRTPSFVRGVINLRGKVIPVVDLRMKFELASQEDTNRTCIIVVQVARG
                     NQSVTMGILVDEVSEVLDINGNQIEPAPKFGDNISTDFILGMGKVAQKVVMLLDVEKV
                     LSGGEIAVVDQIGKDN"
BASE COUNT          343 a          455 c          316 g          357 t
ORIGIN      
        1 cccggtctca agaccaacac ttccctggat aacatagacc ggatcgaccg acagtttctc
       61 cccaagccaa cgcactcttc cactcgattc tgaatagaag ttgcttatat cgccttcgga
      121 ctcacgacga acgttcgggc ctggaaacac cttggcgggt ttccccttct tgaagggaat
      181 gagcttgcca aacacatccg tcccgggttg accatccaga ggggggataa cgcggcacag
      241 aagttcattc tcctggactt cagaggagtc tttgcgctgc cggtagtcaa tggcaccggt
      301 gcgctcgtcg aagacgaaac cggttgtgaa aaagtctctc gcccatttga tctcaccgtc
      361 attgcctgcc acaggctcct gtcccttaac aactaagaga ctcttgatcc tcttgtcctc
      421 ccccagggct tgagagatcg cttctcggag ttgctccaac tcaggaagtt ggacaatctc
      481 tctttcctgc agtgcttcaa ggatctccag aaccaagtct tccagaagac cctcttcgac
      541 ggtgcagtcc aggagaacct gagtgcgatc aggggacacg gtgatcttga acttcttctc
      601 ttcagctgtc ataggctggt cctttgaacg tctcaagacg ttgactcagt cagacaccac
      661 cattgtcatc ttagttgtct ttgccaatct ggtcaactac ggcaatctcc ccgccggaaa
      721 gaactttctc gacatcgagt aacatgacaa ccttctgcgc caccttgccc atgccaagaa
      781 tgaaatcggt ggagatatta tccccaaact tcggggcggg ctcaatctgg tttccattga
      841 tatccagcac ctcagagacc tcgtccacca gaatgcccat ggtcacgctc tgattgccac
      901 gcgccacctg gacaacgatg atacaggttc ggttagtgtc ttcctggctc gcgagttcga
      961 acttcatgcg aagatccaca acggggataa ccttaccgcg aagattgatc accccccgaa
     1021 caaagctggg agtccgaggt acacgagtca cattcatcat cccgatgatc tcctgcacct
     1081 tgaggatttc cagaccgtat gtctcacccc cgagttggaa agtgagatac attccggcaa
     1141 agtcagacat acccatctcc tgacctgact gttcctgatt cactgattta ctaagcgcca
     1201 tctctctact ccttttctgc cttggatgtc tggcattcac atcagaccag accaagacga
     1261 atcaccgtcc gaaggctcta tccttgcacg ccaatctaat cctcccgagt atcgctccct
     1321 cctctcgatg ttcttccatg cgctgtatgc gcgagtttga caacaccacc aacgtccagg
     1381 atcaggccaa ctctgccatc aggcatgatc gcccctcccg caataccctg aacgctgcca
     1441 atgccacttc caagactctt tatgacaatt t
//
LOCUS       DAOQUC010001193         1471 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_120368_length_1471_cov_2.763418, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001193 DAOQUC010000000
VERSION     DAOQUC010001193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1471)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1471)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1471
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120368_length_1471_cov_2.763418"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1446)
                     /locus_tag="PKH07_16815"
     CDS             complement(<1..1446)
                     /locus_tag="PKH07_16815"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNT36651.1"
                     /translation="MCVNGAGTVVWYTDRGGRAVHRMVGTPATGYTEDSGFALNLEDG
                     SAAAGFRAIALSLDETRLFICDNRDQCILVVDSTTGSIQRQFGTSLLGYDLDLDEEGN
                     VYVSSYGTPGRVDKFRYVAPAGEILDNSDPGCTVQGTWSTDATQSYGPNRYTKAAGSG
                     TATVTWTASVTPGWYLVRFWMTSNTTFATNARYSITHRDGISVVVKNQQQTTSNWVHL
                     GGAYYFDGTATVTLNDQFTGGSSVVADAIQLTPVVTMIQMSDSHVGYPTGTANTTAVV
                     NELKTLGRVQLGTFGFEAPPPSFAIHSGDFTEYGQEYWDTLMSIFTGMPLPTYFVQGN
                     HDSTWSSCKERIRSLHGNTPYSFDHYLDGQRLHFACMNSPVLQSPRAGFSREELDWLA
                     ADLAALEPNTLIFISIHHPINGASDPKPFDTYHLLETLRPYRLAIIFYGHGHSFVAPT
                     FDRAQLLQGGSTYNNSTDLGSYAVIAITKDRI"
BASE COUNT          320 a          390 c          426 g          335 t
ORIGIN      
        1 gatccgatct ttcgtgatgg cgatcactgc gtaggaaccc aggtctgtgc tgttgttgta
       61 agtgctgccg ccctgaagca actgcgcacg gtcgaacgtc ggagcgacaa acgaatgacc
      121 gtgaccatag aagatgatcg caagccgata tggtcggagc gtttccagca aatgataggt
      181 gtcgaaaggt ttgggatctg atgctccgtt gatgggatga tgaatactga tgaagatcag
      241 ggtgttaggt tccaatgcgg ccaggtcagc cgcaagccag tcgagttcct ctcttgagaa
      301 tccagcgcga gggctctgca gcacgggtga gttcatacag gcgaaatgca gacgctgtcc
      361 gtccaggtag tgatcgaaag agtagggagt gttcccgtga agggaacgaa tgcgttcttt
      421 gcagctgctc caggtcgaat cgtgattgcc ctgaacaaag taggtcggca aaggcattcc
      481 ggtgaaaatg ctcataagcg tgtcccagta ctcctgaccg tattcggtga aatcccccga
      541 gtgaatggcg aaactgggtg gcggcgcttc aaagccaaaa gtccccagtt gaacccttcc
      601 gagagtcttg agttcattga caacagccgt tgtgttagcc gttccggttg ggtagccaac
      661 atgggagtca gacatctgaa tcatggtaac gacaggtgtg agctggattg catcagcaac
      721 gaccgatgac ccgcctgtaa actgatcgtt cagggtcacc gttgctgttc catcgaaata
      781 gtacgctcca cccagatgta cccagttgga tgtcgtctgc tgctgattct tcacgaccac
      841 gctgatgcca tcacgatggg tgatcgaata gcgggcgttg gtggcaaagg tggtgttgct
      901 ggtcatccag aaccgaacca gataccaccc tggagtcaca gaagcagtcc atgtgaccgt
      961 ggctgtacca ctgcccgctg ccttggtata tcggttggga ccgtaggatt gtgttgcgtc
     1021 tgtgctccag gtgccttgca cggtgcatcc cggatcggag ttgtccagaa tctctcccgc
     1081 cggagcgacg taccggaact tgtccactcg acccggagtg ccgtaggaac tcacgtagac
     1141 gttgccctct tcgtccaagt caagatcgta gcccaacagg cttgtcccga attggcgctg
     1201 aatcgagccg gttgtcgagt ctacaaccag gatgcattga tccctgttgt cacagatgaa
     1261 aagccgcgtt tcatccagac tgagagcaat cgccctgaag cccgccgccg ctgatccgtc
     1321 ttcaagattc agcgcgaaac cggagtcttc cgtgtatccg gttgcgggag ttcccaccat
     1381 tcgatggacg gctcttccgc cgcggtcggt gtaccagact accgtgcccg cgccgttgac
     1441 gcacattccc tcatgagcgt ctgcgggtgt t
//
LOCUS       DAOQUC010001194         1467 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_120882_length_1467_cov_2.888810, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001194 DAOQUC010000000
VERSION     DAOQUC010001194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1467)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1467)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1467
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120882_length_1467_cov_2.888810"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..851)
                     /locus_tag="PKH07_16820"
     CDS             complement(<1..851)
                     /locus_tag="PKH07_16820"
                     /inference="COORDINATES: protein motif:HMM:NF024930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36652.1"
                     /translation="MNTTRVADSSRLGSFHSGLLRLLAMTLLLVCTFESAFAIQSGAI
                     VGWGSQIVGAELGSGFVRVAAGDSHSLALKEDGSIVAWGRNDYGQCDVPSPNSDFIRI
                     AASCDNSFGIKSDGSVVAWGRSTPALNVPVPNTGFTEIAAGATGENVVALKEDGSLVC
                     WGWTFQGGWAPPSPNSDFIAIAAGREHGLALRSNGSIACFGYNSGGCCTVPLPNENFV
                     AVAAGRTHSLGMKSDGSVVCWGTDNSGWGQLSVPSPNSGFTVIGGGNFFSVGIREDTS
                     VAVWGANV"
     gene            complement(1047..>1467)
                     /locus_tag="PKH07_16825"
     CDS             complement(1047..>1467)
                     /locus_tag="PKH07_16825"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HNT36653.1"
                     /translation="VNLLRQLGPLAEKRGVTITVEPLNKAECNFITSLGEGATIVEQC
                     DHPNVALLADIYHMTQNGQGPEDIIQFGKHLRHVHIAEKEKRTAPGTMGDDFRPFFKA
                     LKHVGYDARISIEGRWDNLANQAAGSIGVLRQQWTRS"
BASE COUNT          362 a          424 c          348 g          333 t
ORIGIN      
        1 acatttgcac cccaaacagc gactgaagta tcttctcgaa tcccaacact gaagaagttg
       61 cctcccccaa taactgtaaa accagagttc ggagacggta cactcagttg cccccaaccg
      121 ctattatctg ttccccaaca cacgacggag ccgtcggatt tcatcccaag gctatgtgtt
      181 ctccctgcgg cgacggctac aaaattctcg tttggaagag ggacggtgca acatccacca
      241 gagttatagc caaagcaagc gatcgaacca ttggatctca gagcaagccc gtgttctctg
      301 cctgcggcga tcgctatgaa atccgagttg ggtgaaggtg gggcccatcc cccctgaaaa
      361 gtccagcccc aacaaaccaa ggaaccatct tccttcaaag ccactacatt ctcacctgta
      421 gcacctgcag caatctcagt aaaccccgtg ttcgggacag gaacgttcag ggcaggtgtg
      481 cttcttcccc acgccacgac tgacccgtct gacttgatgc cgaagctgtt gtcacaagac
      541 gccgcaattc tgatgaagtc agaattgggc gatggcacgt cgcattgtcc atagtcattt
      601 cgtccccaag ccactatgga tccgtcttct ttgagcgcca gactgtggga atctcctgca
      661 gcaaccctca caaaccccga acccaattcc gccccgacga tctgagaacc ccaaccgaca
      721 atcgcaccgg actgaatcgc aaatgcggat tcgaaggtgc agaccaacag caatgtcatt
      781 gccaaaagtc tcagtaaacc tgaatgaaaa ctacccagac gtgaagaatc tgcgacgcgt
      841 gtcgtgttca tgctttcctc cattgcactt cagcacagaa ttcagccaac atggactggg
      901 acaacaacag caagccacga gaggcgaaaa cactcaccaa ggttgtgcag cagagctccc
      961 tgtctacact gtaagagatc gctctccggc agcactatcc ccattccagt aacttacact
     1021 cagagtactt tggttgagca aagaagtcaa gatcgagtcc actgttgtcg gagcacaccg
     1081 atgctccccg ctgcctggtt ggcaaggttg tcccatcgtc cttcgattga gatgcgagcg
     1141 tcgtagccga cgtgtttgag tgccttgaag aagggtcgaa aatcatcgcc cattgtgcca
     1201 ggtgcagttc gtttctcttt ttcggcaatg tgaacgtgcc tgagatgttt tccgaactga
     1261 atgatgtcct ctggtccttg gccgttttgg gtcatgtgat agatgtccgc caggagagcg
     1321 acgttgggat gatcgcattg ctccacgatc gttgctccct ctcccagtga tgtgatgaag
     1381 ttacattcag ccttgttgag cggctcgacg gtgatggtga cccccctttt ctcggcaaga
     1441 ggtccaagtt gtctcagcaa attgaca
//
LOCUS       DAOQUC010001195         1467 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_120913_length_1467_cov_2.268414, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001195 DAOQUC010000000
VERSION     DAOQUC010001195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1467)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1467)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1467
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120913_length_1467_cov_2.268414"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..761
                     /locus_tag="PKH07_16830"
     CDS             <1..761
                     /locus_tag="PKH07_16830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36654.1"
                     /translation="KERLLQMILTVTVVSVCPVYAQQPPEPPKPDLYKTSQESIARLK
                     QIGESFRMLDKRLDEIRNDLEAYHERREGFGRGPESRDSLLPRIYERRQRGSGMAQLH
                     ELARERIRVAREKEELFKEARRLYEETLEGMDKEIADLQSQQPSPEQDHKIEKILQAK
                     RDERLKFEKAVQDIASEVISEATRQRLGPRLWQGERPSFERRDRESPDIARRVEELEN
                     RLDLLEDLLLEVAQKVGCATDFPARQRMVPGRMR"
     gene            complement(790..1236)
                     /locus_tag="PKH07_16835"
     CDS             complement(790..1236)
                     /locus_tag="PKH07_16835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNT36655.1"
                     /translation="MRTIRFSSIVNAPREKVFDFYEKVESLMQITPPKMAFWAESDHE
                     RMVKGAEIILHFRVLRLFSIRWVKKIVESMPPERFEDIQESGPLQYWHHKHLFVQHGI
                     QTEVIDEVDYELPLGFLGALIDLVCIRRVLNRMFRFRHRRTTEIFG"
BASE COUNT          389 a          379 c          384 g          315 t
ORIGIN      
        1 tgaaagagcg ccttctccag atgattctca cggtgacagt tgtttctgtg tgtccggttt
       61 atgcgcaaca accgccagaa ccacccaagc ctgatttata caagacatcc caggagtcca
      121 tcgccaggct caaacagatt ggcgaatctt ttcgcatgct ggacaagcgg ttggatgaga
      181 tccgaaatga tctggaagcc tatcatgaac gacgtgaggg attcggacgc ggccccgaat
      241 cccgagactc actcctgcca aggatctatg agcgtcgtca gaggggttcc ggaatggcgc
      301 agttgcacga actggcgcgt gaaaggatac gcgttgctcg cgaaaaggaa gagcttttca
      361 aggaagctcg gcgactttac gaagagacac tcgaaggaat ggacaaagag atcgccgatc
      421 tccagtcgca acagccttcg cccgagcagg atcacaagat cgagaagatt ctccaggcca
      481 agcgtgacga acgcctcaaa tttgagaaag cggtacagga cattgcgtct gaggtgatct
      541 ctgaggcaac aagacagagg ctggggccac ggctctggca gggagaacgt ccctcattcg
      601 agagacgaga tcgcgaatca cccgatatcg ccagacgggt ggaagaactc gagaaccgcc
      661 tcgatcttct cgaggatctg ctgctcgagg ttgcccaaaa ggtaggttgt gcaaccgatt
      721 tccccgctcg ccaacgcatg gttcccggta gaatgaggtg aagccggggt acttccccct
      781 cgcgcgtttc taaccaaata tctcagtggt tctgcggtga cgaaatcgaa acatccgatt
      841 caggacgcgt cggatacaga ccaagtcaat gagagcacca agaaaaccca gcgggagctc
      901 gtagtcaact tcatctatca cctcagtctg aatgccatgc tgaacaaaga gatgcttgtg
      961 atgccagtat tgcagtgggc cagactcctg gatatcctca aaccgctcgg gcggcatgct
     1021 ctcgacgatc ttcttaaccc atcgaatgga aaacagtcgc agaactcgaa aatgcagaat
     1081 gatctccgct cccttcacca tacgctcgtg gtctgattca gcccagaaag ccatcttcgg
     1141 tggtgtgatc tgcatgagag attccacctt ttcatagaaa tcaaagactt tctcacgtgg
     1201 agcgttgaca atgctcgaaa atcgaatcgt tctcatcact ggtacctgcc gattcagtgt
     1261 tttcggagag gttaagctga atcaggaaac cgtacaagcg caaacaacct gagtttccta
     1321 tcaaagattt tcgcggtaga ccgacagagg aatgtgcggt aaggttcttt cgtcggtgtt
     1381 ctttaccgaa gacaccacac aacatatacg aaccagagcc gatgctgcgg ttttggtttt
     1441 ctatcaaggg gctgtcacca tgaaagg
//
LOCUS       DAOQUC010001196         1466 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_120982_length_1466_cov_3.347271, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001196 DAOQUC010000000
VERSION     DAOQUC010001196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1466)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1466)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1466
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120982_length_1466_cov_3.347271"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..486
                     /locus_tag="PKH07_16840"
     CDS             <1..486
                     /locus_tag="PKH07_16840"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HNT36656.1"
                     /translation="STFDGVSLAWAIAEYIHNLRGKGVKTLFATHYHELADLPRKLSR
                     AANYRVLIAEKEGTVTFLYKIASGSTDHSYGIHVAELAGVPERVIKRARRILEDLEGH
                     ASVLRDKSEAQEAESLQMSLFSLIEEPIANRIRDLDLDAMTPLEALQFLSDLAREVRA
                     K"
     gene            574..1194
                     /locus_tag="PKH07_16845"
     CDS             574..1194
                     /locus_tag="PKH07_16845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36657.1"
                     /translation="MDVYVALTGLPACGKETFKDLLFLAATERGFQNHHISFTDVLRD
                     ECVRRGVECNRENYTNIANALRSEFGSDILAERIVEKVQGVQAQQQASRLFVLEAVRN
                     PREADLFRSRLGNGFALVGITAPRELLIERIRTRKRLDEQPGVMDSRAAAEALLDREW
                     GVNQPETGLRVGDCLAVSDFLIDNSGDLQSLEMQVRRLLARLRGEA"
     gene            complement(1211..>1466)
                     /locus_tag="PKH07_16850"
     CDS             complement(1211..>1466)
                     /locus_tag="PKH07_16850"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HNT36658.1"
                     /translation="DPAPIAVFKRFGESSLEFELRVFIGRMDHFLGTSHDLHMAIDKA
                     FREVGIEIAFPQRDLHVRSAKGLASLLGQSGLDIPSQGRK"
BASE COUNT          338 a          362 c          437 g          329 t
ORIGIN      
        1 agtacattcg atggagtgag tctggcatgg gcgattgctg agtatattca caacctgcgt
       61 gggaaagggg tcaaaacgct tttcgccacg cattaccatg aacttgcgga cctgcctcgg
      121 aaactctcca gagccgccaa ctatcgggta ctgattgcgg aaaaagaggg tacggtcact
      181 tttctctaca aaattgcgtc tggcagcaca gatcacagct acggcatcca cgttgccgag
      241 ttggcgggcg ttcccgaacg ggtgatcaaa cgggcgagac ggattctcga ggatctcgaa
      301 ggccatgctt cggtgttgcg ggacaagtct gaggcacagg aggcagagtc actccagatg
      361 tccctttttt ctttgatcga ggaaccgatt gccaacagga ttcgagattt ggatctggat
      421 gccatgacgc cacttgaagc gctgcagttt ctgtcagact tggcgagaga agtccgagcg
      481 aagtaaaagt cgcgagtcaa gtgactccat gtcacaggga ttctatccgt ggccaagggt
      541 agtttcggtg cggccatttt ggaggtgtgt ttgatggatg tgtacgttgc actgacaggt
      601 ttgcctgcat gtggcaagga aaccttcaaa gacctgctct ttctcgcagc taccgagcgg
      661 ggttttcaga accatcatat cagtttcacc gatgtgcttc gggatgagtg tgtccgtcgc
      721 ggggtggaat gcaacaggga aaactacacc aacattgcca atgcgctccg atcagagttt
      781 ggctccgata tcctcgccga acgcatcgtc gagaaggtgc agggtgttca ggcacaacag
      841 caggcatctc gtttgtttgt tctggaggcg gttcgaaatc cgagggaagc cgatctgttc
      901 cggtcacgtt tggggaacgg ctttgcactg gtcggcatca ccgcaccgcg ggaactactc
      961 atcgagcgta tccgaacaag aaagcgcctg gatgaacaac ccggtgtgat ggactcgcgc
     1021 gctgcggctg aagcactgtt ggatcgggag tggggtgtga atcaacctga gacgggtctg
     1081 cgcgttggag actgccttgc tgtctcggat ttcctgatag acaattcggg ggatctccag
     1141 tccctggaga tgcaagtacg acggctgctt gcgaggctca ggggcgaggc gtgatcagag
     1201 gattcgccga ctatttccga ccttgcgatg ggatgtccaa gccggactga cccaacaatg
     1261 aagccagacc ctttgcagat cggacatgca gatcccgctg cgggaatgca atctcgattc
     1321 ccacctctcg aaaagcctta tcaatggcca tgtggagatc atgggacgtt ccaagaaaat
     1381 gatccatgcg gccgatgaaa acacggagtt caaattccag actgctttcg ccaaaacgct
     1441 tgaagacggc gatgggggca ggatcc
//
LOCUS       DAOQUC010001197         1464 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_121345_length_1464_cov_2.224273, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001197 DAOQUC010000000
VERSION     DAOQUC010001197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1464)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1464)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1464
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121345_length_1464_cov_2.224273"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1464
                     /locus_tag="PKH07_16855"
     CDS             <1..>1464
                     /locus_tag="PKH07_16855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36659.1"
                     /translation="GGKGDTPISLDGSIIYALDMARAKEYPWQCSIIKQQHDLNLVCF
                     PCRAFTLVGLTDPRGYIVLKDLIILEAGTQSPPFEYGQSATDFFWGNPEESCVTLWAD
                     PTIRVRLMLGLGFQGKRLVLVDNDLENPIGNGFVLEQLDTIPSMVLQGARDMWNLDET
                     RLRKLEFNGVKNPRIKQIHLEAQQSLQQAEASLKAHDYRGYRSASERGWSLESKAYGE
                     ILATINNMIRGVLFYLALLMPFSYCLERLLFASNTIRKRIVLMGVIFVVSFTVLAMVH
                     PAFRFTMTPLIVLLAFVILALAGTVSTLIVTKMDGLLQERKQASIGVHEDTHNVGNVA
                     VRSVDLGVANIRRRPQRGILTGATIILVTFILLSFTSLVPVLNIAKVKHPEGETTYKG
                     LLVRDRKWLPLPSPLRSSLQRNYSENGAQQTQLPPDSIVAARAWFFSDYSGNLSQIDL
                     SPARLGEASNEISDAPQKDRKFFTAVSLLCMEHTEPAI"
BASE COUNT          334 a          395 c          372 g          363 t
ORIGIN      
        1 ggtggaaagg gagacactcc catctccttg gatggttcaa tcatctacgc cctcgatatg
       61 gcgcgtgcca aagagtaccc ttggcaatgc agtattatca agcaacagca cgatctgaat
      121 ttggtctgtt ttccctgccg ggctttcact ttggtggggt tgaccgatcc tcgtggatac
      181 atcgttctca aagatttgat catcctggag gcaggcaccc aaagccctcc ctttgaatac
      241 ggacagagtg caacggattt cttctgggga aatcccgagg agagttgcgt cacgctctgg
      301 gcggatccga ccatcagggt tcgtctgatg ctcgggttgg gatttcaagg caaacgcctt
      361 gttcttgtgg ataacgatct tgagaatcct atcggaaacg gctttgttct cgagcaactc
      421 gatacaattc cctcgatggt tctccaaggc gcacgcgaca tgtggaatct ggatgaaacg
      481 cgactgcgca aattggagtt caacggcgtc aagaatcctc gaatcaaaca gattcatctt
      541 gaagcccagc aaagtctcca gcaagctgaa gccagtctca aagcccacga ctaccgcggc
      601 tatcggagtg caagtgagcg tggatggtcg ctggagagca aggcctacgg cgagattctg
      661 gcgacgatca acaacatgat ccgaggagtg ctgttctatc tggctctcct gatgcctttt
      721 tcctattgcc tggagcggct cttattcgcc tccaacacca ttcgcaagcg tattgttctc
      781 atgggcgtta tcttcgttgt cagtttcacg gtgctggcga tggttcatcc tgcttttcgg
      841 tttacgatga caccactgat tgtgctgctt gcattcgtga ttcttgcttt ggctggaacc
      901 gtgagcactt tgattgtcac aaagatggac ggtcttctgc aggaacgaaa gcaggcgagt
      961 atcggtgtcc atgaagacac acacaacgtc ggaaacgtcg ctgttcgttc cgtagatctc
     1021 ggtgttgcca atatccgacg ccgcccacag aggggcatac ttacgggagc cacgattatc
     1081 ctcgtgacgt tcatcctcct ttccttcact tccttggtgc cagtcctgaa catagccaag
     1141 gtcaaacatc cggaaggcga aacgacgtac aaaggactcc tggttcgaga ccggaagtgg
     1201 ttaccactcc caagtcctct tcgatcgtcc ctgcagagaa actacagcga gaatggggca
     1261 cagcaaacgc aacttccacc ggactccatc gtcgctgcgc gcgcttggtt cttttcggac
     1321 tattctggaa atctctcaca aattgatcta tcccctgcac gacttggtga ggcctcgaat
     1381 gagatttcgg acgctccaca gaaagaccgg aagttcttca cagccgtctc gctactctgc
     1441 atggagcaca cagagcctgc aatc
//
LOCUS       DAOQUC010001198         1463 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_121416_length_1463_cov_3.222301, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001198 DAOQUC010000000
VERSION     DAOQUC010001198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1463)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1463)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1463
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121416_length_1463_cov_3.222301"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..395)
                     /locus_tag="PKH07_16860"
     CDS             complement(<1..395)
                     /locus_tag="PKH07_16860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019535429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar isomerase"
                     /protein_id="HNT36660.1"
                     /translation="MDQKWTKRYEAFAEHQAARGIDLQSVKKSILAHHIETPSWGYAD
                     SGTRFGTFSQAGAARTAQQKLADAAMVHRLTGVCPSVALHIPWDKVDDYAALSQYAAS
                     LGVTLGAINPNLFQETEYKMGSLCNPSEKV"
     gene            complement(710..>1463)
                     /locus_tag="PKH07_16865"
     CDS             complement(710..>1463)
                     /locus_tag="PKH07_16865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36661.1"
                     /translation="LSAEVADQFPNLYSPNGVAVDAASQNAVRMRVRMSAGDKVRVFY
                     RRQGETTYAGYREATLTSNASFVNCWMDFSQDVSYTGTIDRFRVAPTGAPNGAKLELD
                     FFRVLQPTYQFTWRWDFDEQGNSEFWTALKHITPLQVAGGILSATTTGDDSCLTSRAG
                     LNLDASTRQWVIIRMKVSAGTYADLFYKAPAEGFSAAKRRRFDLTGNTDFATYTLDMS
                     NAPGWSGTIDRLRLDPTVVSGATVEIDYVLVP"
BASE COUNT          331 a          394 c          390 g          348 t
ORIGIN      
        1 actttctcag atgggttgca gagactcccc atcttgtatt cggtttcctg gaaaagattc
       61 ggattgatgg caccgagcgt tacacctaag gacgccgcat actgtgacaa ggcggcgtag
      121 tcatccacct tgtcccaggg aatgtgcaag gcgacagaag gacaaacacc ggtcagtcgg
      181 tgaaccatcg ccgcatcggc aagcttctgt tgcgccgttc gagcggctcc agcttgcgaa
      241 aacgttccaa atcgggttcc ggaatcagca tatccccatg agggggtctc aatgtggtgt
      301 gcgaggatac tcttcttgac cgattgaagg tcaatgcctc gcgcggcctg gtgttctgca
      361 aaagcctcgt atcgtttcgt ccatttctga tccatggtca cagtcaaaaa cctcccaatg
      421 gcatgatatg tgtggatggt aacggtttga aggtgtgaga gtcaacagcg atttgccaca
      481 gcgtgaaacg ccacccgatc tcaaggaaca tcagcccctt gtccgcgttc gtatcattcg
      541 ggagttctct tgcagcacca cgtgttcgcg aggctcttcc catttgacag aatgccgaac
      601 aggcgctcaa cgatcccagc cagtgtggtc ttctgggatg gttccatggc cagtggttaa
      661 cgtctgatct gggcgtcttc atgaggagga agattgccct tctcaacgct tacggaacca
      721 gtacgtaatc tatctcgacc gttgcaccag agactacagt tgggtcaaga cgcaagcggt
      781 caatggtgcc tgaccaaccc ggagcgttgc tcatgtcgag agtataggtt gcgaagtccg
      841 tattgcccgt caggtcaaag cgccttctct ttgccgcgct gaatccttca gccggtgctt
      901 tgtagaaaag atccgcgtat gttcccgcgg agactttcat cctgatgatc acccattgcc
      961 tggtcgaagc atccagattc agacctgcac gagaggtcag gcaagaatcg tcacctgttg
     1021 tggttgcgga gaggatgcca cctgcaacct ggagcggagt gatgtgtttc aacgctgtcc
     1081 agaactcact gttgccctgt tcatcaaaat cccagcgcca cgtgaattgg taagtcggct
     1141 gaagcactcg gaagaagtcc agttccaact tcgctccatt gggagcacct gttggagcaa
     1201 cgcggaagcg atcgatcgtg ccggtgtaag aaacgtcctg agagaagtcc atccagcagt
     1261 ttacgaaact cgcattgctt gtgagagtcg cctctctgta acccgcgtat gtggtttcgc
     1321 cctgtctgcg atagaagact ctcactttgt caccagcgga cattctcacg cgcatacgaa
     1381 cagcgttctg tgaggccgca tccacggcaa caccatttgg ggaatagaga tttgggaact
     1441 gatcagccac ttctgcactc aac
//
LOCUS       DAOQUC010001199         1463 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_121467_length_1463_cov_2.526989, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001199 DAOQUC010000000
VERSION     DAOQUC010001199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1463)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1463)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1463
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121467_length_1463_cov_2.526989"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..780)
                     /locus_tag="PKH07_16870"
     CDS             complement(<1..780)
                     /locus_tag="PKH07_16870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36662.1"
                     /translation="MRIRITSLISALIVGLCLFTPLAWSQNDQMRISAEADFRISGTT
                     FADHEIAGTDLSGNWSNQLAAMTSLLPSQVGIDALDITSETEVYLSLDVDTQIGGTLY
                     ANEDVLRWDGVGLTMTWDGTANGLPTRANLSALHVISDSPWEMAFSLSATTQLSGLGL
                     VRPSDAVHYVEGSGFVGFFFQGQSAGLPAGVKVDMFQIWTADHYLISFDCAVRPTGHV
                     TTYRSADILSWDPDSGTLSASPFFSADAQSLPARIDINAAVA"
     gene            complement(887..>1463)
                     /locus_tag="PKH07_16875"
     CDS             complement(887..>1463)
                     /locus_tag="PKH07_16875"
                     /inference="COORDINATES: protein motif:HMM:NF016906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NosD domain-containing protein"
                     /protein_id="HNT36663.1"
                     /translation="TCTNNSGTGNGSGYGIYVSGDSTLSFNTCTSNNGQGENSSGIGI
                     RVHEGSTITNNTCTYNRGSYSSGSGSTGAGIVVTGAGNTISHNNCDANTGNGLQSSAG
                     IRITGNGNRIENNSLTRHSGGIGSYGLSVSGTRNHLLSNTFQGNTTNSIYISGSNNYY
                     AANIHPAAESISDGGTGNVNGTMPGSNVAVP"
BASE COUNT          320 a          394 c          387 g          362 t
ORIGIN      
        1 agccacagct gcattgatgt caattcgggc cggaagactc tgagcgtcgg cactaaaaaa
       61 aggcgatgct gacaaagtcc cgctgtccgg atcccaggaa agaatatcgg cgctgcggta
      121 ggttgtgaca tggccagtcg gtctcaccgc acaatcgaag gaaatcaggt aatggtctgc
      181 agtccagatc tgaaacatat ccaccttgac tcccgcaggc aaaccggcgc tttgaccttg
      241 aaagaagaag ccgacaaatc ctgatccctc aacatagtga accgcatccg acggtcgaac
      301 cagccccagg ccggacaact gagttgtggc gctaagggaa aatgccatct cccagggaga
      361 gtcactgatc acgtgaaggg cgctcaggtt tgctcgcgtc ggaagtccgt tggcagtgcc
      421 atcccacgtc atagtgagac cgactccatc ccatcggaga acgtcctcgt tggcgtacaa
      481 tgttccgccg atctgtgtgt ctacgtccag cgacagatag acttccgttt ccgacgtgat
      541 gtccaaggcg tctatgccca cttggctcgg cagcaatgaa gtcattgctg ccagttggtt
      601 gctccaattg ccagatagat cagttccagc gatctcatgg tctgcgaatg tcgtgcctga
      661 gatacgaaaa tccgcctcag ctgagatccg catttgatcg ttttgactcc aggccagcgg
      721 cgtaaacaaa caaagtccca ctatgagcgc gctgatgagg gaagttatcc gtattctcat
      781 gtttgtcgct tctcctgctg cattccccct gccagagtct tgtatcgtct cggcttctgt
      841 gtacccactt ggcttgtaat cgccacgttt gtccgacaat gttcgctcac ggtaccgcga
      901 cattgcttcc cggcatagtt ccgttgacat ttccagttcc cccgtcggag attgattcag
      961 cggctggatg gatgtttgca gcgtagtagt tgttgctccc gctgatgtag atactgtttg
     1021 ttgtgttgcc ctggaacgta ttgctgagaa gatgattgcg cgtccccgaa accgaaagtc
     1081 cataactgcc aatgccgccg ctgtggcgag tcaaactgtt gttttcaatg cgattcccgt
     1141 tccctgtaat acggatacca gcagaagact gcagcccgtt gcctgtgttg gcatcacagt
     1201 tgttgtggct gatcgtattg cccgccccgg ttaccacaat tccagcaccg gtggaaccag
     1261 atccggagct gtaactcccg cggttatagg tacaggtgtt gttggtgatg gtggagcctt
     1321 catgaactcg aatgccgatg ccggaagagt tctcgccctg accattgttg gatgtacacg
     1381 tgttaaagga gagggtgctg tcgccggaaa catatattcc gtatccactt ccattaccgg
     1441 ttccagaatt gttggtgcag gtg
//
LOCUS       DAOQUC010001200         1462 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_121587_length_1462_cov_2.697939, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001200 DAOQUC010000000
VERSION     DAOQUC010001200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1462)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1462)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1462
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121587_length_1462_cov_2.697939"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..854
                     /locus_tag="PKH07_16880"
     CDS             <1..854
                     /locus_tag="PKH07_16880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36664.1"
                     /translation="VRVANGVLWGTSIGTDPWYRTEPGLNLNADDVRWLEIKMKVTQG
                     KSADLFFKSASDPVFTSAKRIVLNIPRNDVSLVYRVDLSAVIEWKGVIDRLRLDPTNA
                     VDAVIHVDYIRFSSATAAPTYTPTGAATSTPTATPTVTPSVSPVVWEFNERDNFEGWS
                     ANAQLTDVRVAGGLLQGISTGIDPWYRTGTGLSLKASDYPWLEVRLKATAGTTAELFF
                     KSAEDLMFTAAKRVTLPVPTNDSYITYRVHLASFGGWMGVIDRLRLDPSNAVSNVYVD
                     YIRLLPN"
     gene            942..>1462
                     /locus_tag="PKH07_16885"
     CDS             942..>1462
                     /locus_tag="PKH07_16885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009596508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 domain-containing protein"
                     /protein_id="HNT36665.1"
                     /translation="MRLTGTFLDEISHDIPHQNWGKQEWDRDFAAMKAIGIDTVILIR
                     CGWKRWMTFPSKVLQEERSGFAPPVDLVRLFLDLSEKHGLSFYFGTYDSGEFWHQGKY
                     QEEVDLCRKVVDEVWCLYGDSPAFKGWYLSQEVSRKVHSIVDLYASLGRHCKAISGGL
                     PVLISPYIQGVKAV"
BASE COUNT          320 a          363 c          421 g          358 t
ORIGIN      
        1 atgtccgtgt cgccaacgga gtcctctggg gtaccagcat cggcacggat ccctggtatc
       61 ggacggagcc cgggctgaac ctgaacgctg atgatgttcg ctggcttgaa atcaagatga
      121 aggtgacgca gggcaagtct gccgatctgt ttttcaaatc tgcttcggat cccgtgttca
      181 catcggccaa acggatcgta ctaaacattc caaggaacga tgtttctttg gtctaccgtg
      241 tggacttgtc tgccgtgata gagtggaaag gagtcattga ccggctgaga ctggatccga
      301 caaatgccgt tgatgctgtc attcatgttg actacattcg tttctccagc gctacggcag
      361 cgcccaccta tacgccaacg ggagcagcga catccacacc caccgccacg ccaacggtga
      421 cgccatccgt ttcgccagtg gtgtgggagt tcaatgagag ggataacttc gaagggtgga
      481 gcgccaatgc ccaactgaca gacgtccgcg ttgccggtgg cctgcttcag ggcatcagta
      541 ctggcattga tccgtggtac cggacgggga ctggtctgag tttgaaggca tctgactatc
      601 catggcttga agttcggctc aaagccacgg caggcacgac ggctgagttg tttttcaagt
      661 ctgccgaaga tctcatgttc actgccgcaa agcgcgtgac tctgcccgtt ccgacaaatg
      721 attcctatat cacctaccga gtgcacctag cttcgttcgg cgggtggatg ggagtcattg
      781 accgattgcg tctcgatcca agcaatgccg tttcaaacgt ttatgttgac tacatacggc
      841 tcttgccaaa ctagccttgg atggggcaca atcccgcgag gttgttcttc cctggtgtct
      901 cacgacaccc agtgtgaatt ttttgggagg ttgcagagat aatgcgactg acaggcactt
      961 ttctggatga aatcagtcac gacattcccc atcagaactg gggaaaacag gagtgggatc
     1021 gcgattttgc agcgatgaag gccattggca ttgatacggt cattctgatc cgatgtgggt
     1081 ggaaacgttg gatgaccttc ccgtcaaaag tacttcagga agagcgttcg ggctttgcgc
     1141 caccggtgga tctggtgcgg ctgttcctgg acttgtccga aaaacacggt ctctcgtttt
     1201 actttggtac ctacgattcg ggagagttct ggcatcaggg aaagtatcag gaggaagtgg
     1261 atttgtgccg gaaagtggtg gacgaggtct ggtgtcttta cggagacagt ccggcattca
     1321 agggatggta tctgtcacag gaagtcagca ggaaagttca ttcgatcgtt gatctgtatg
     1381 ccagtctggg caggcactgc aaggccatct cagggggact tcccgtcctg atttcccctt
     1441 acatccaagg agtcaaggcg gt
//
LOCUS       DAOQUC010001201         1461 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_121677_length_1461_cov_4.376956, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001201 DAOQUC010000000
VERSION     DAOQUC010001201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1461
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121677_length_1461_cov_4.376956"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..617
                     /locus_tag="PKH07_16890"
     CDS             <1..617
                     /locus_tag="PKH07_16890"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNT36666.1"
                     /translation="EILLGSTPLSRMSRHQIAREIAVVPQDTSLGFPFSVLEVVLMGR
                     NPFTGMLGLESEQDYKLAFESMERLDIIHLAERRFDSLSGGEKQRTVLARALTQTPHI
                     LLLDEPGTHLDLSHQIVIHEILQNLSREGITVLSITHDLNLAAEFFPRMIAMKQGRLI
                     ADGLTEEVLTESCMQEVFGVRTFVDKNPISGRPRISILKQDSVA"
     gene            complement(699..1352)
                     /locus_tag="PKH07_16895"
     CDS             complement(699..1352)
                     /locus_tag="PKH07_16895"
                     /inference="COORDINATES: protein motif:HMM:NF014010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoU domain-containing protein"
                     /protein_id="HNT36667.1"
                     /translation="MFKEILSFWTRDDLLKEAYDEANQMLETVRDMFVTVSGSLLSGQ
                     KVEMDIHKRDKQVNEMERQVRRKVLEHLSINPKQDVAASLVLTSVVIDIERIGDYSKN
                     VKELVDLYQQEFADVQYMLDLRQMTRSVEDIFSLVAESFKNGDVEKARLAIGSHQAVK
                     NCCDSVIEHVFREQESDTKRAVVGVLYARYLKRVSAHLKNIATGVANPFDMIGYREK"
BASE COUNT          330 a          403 c          363 g          365 t
ORIGIN      
        1 gagaaatcct cctgggcagc actcccctgt cgcgtatgag ccgtcatcag atcgcccgcg
       61 aaattgctgt ggttccgcag gatacctctt tgggctttcc tttcagcgtg cttgaagttg
      121 tgctcatggg gagaaatccg tttacaggga tgcttggtct ggaaagtgag caggactaca
      181 aactcgcttt tgagtccatg gagcgcctgg acatcatcca tctggccgag cggcggtttg
      241 actcgctctc gggtggggag aagcaaagga cagttctggc gcgagcgctg acacagacac
      301 cacacattct gctacttgat gagccaggaa cgcatcttga tctgtctcat cagatcgtca
      361 tccatgagat tttgcagaat ctctcacggg aagggatcac ggttctgtcc atcacccacg
      421 atctgaatct tgcggcggaa ttcttcccgc gcatgatcgc gatgaaacag ggacggttga
      481 tcgccgatgg actgaccgag gaagtgctca ctgaatcttg catgcaggag gtttttggag
      541 tgcggacttt cgtggacaag aacccaatat cgggaagacc gagaatctcc attctcaaac
      601 aggattccgt cgcctgattc gctttcgtcg caaatcaacg tgttccgttt gaagcgctga
      661 tgcagattcg ttcagccaag gaaaccatgc aaggcgcact acttttcccg atagccaatc
      721 atgtcgaagg gattggccac tcccgttgcg atattcttca ggtgggcgct cactcgcttc
      781 agatatcgag cgtagagaac gccgacgacc gcgcgcttag tatcggattc ctgttcccga
      841 aaaacgtgtt cgatgacgct gtcgcagcag ttcttaacgg cctggtgtga acctatggcc
      901 agccttgcct tttcgacgtc tccgttcttg aagctctctg caacgagcga gaagatatct
      961 tccaccgagc gggtcatctg tcgcaaatcc agcatgtatt gaacatcggc aaattcctgc
     1021 tggtaaagat ccaccagttc cttgacattt ttcgaatagt ctccgatgcg ttcgatgtca
     1081 atgaccacgc tggtcagcac gaggctcgcc gccacgtcct gcttggggtt gatcgaaaga
     1141 tgctccagca ccttgcgccg tacttgccgc tccatctcat tgacctgttt gtcacgtttg
     1201 tgaatgtcca tttcgacctt ctgtcccgag agcagacttc ctgacaccgt gacaaacatg
     1261 tccctgactg tctccaacat ctggttggct tcgtcgtagg cttccttcag caaatcatcc
     1321 cttgtccaga aactcagtat ctctttgaac atactttttc acctacctgc ccaggagaaa
     1381 tatgatgaca cccggcaccg tgtagaaaac gacaataatg tagacaaacg cccacacgcg
     1441 attgcggtaa gcaagatccc c
//
LOCUS       DAOQUC010001202         1461 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_121760_length_1461_cov_2.345661, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001202 DAOQUC010000000
VERSION     DAOQUC010001202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1461
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121760_length_1461_cov_2.345661"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(175..>1461)
                     /locus_tag="PKH07_16900"
     CDS             complement(175..>1461)
                     /locus_tag="PKH07_16900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012924111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36668.1"
                     /translation="AATELQLMAGLTAQTPQIKAVNSDASRIVFGTAQGDPDTDLTPP
                     TILDLDVPQSAKYLSGAALTAQIMDQSEVADVTAAYMTGMTSCESEVQLLLLNATQDM
                     FRSPSILFSETGTWTFTVMASDVVGNLSKPLQAYCVVTPDNRLDASELFVLAGEIGNP
                     NSLIDFTGDGILNLDDILAFIGGWHSSRPLPARTPTQTPTTTPTATVTNTPTITPSAT
                     VTPTTTSTDTPTQTDTPTWTPTMTPSDSPTQTPTVTPTATFTLTMTPTRTPTASNTPT
                     VTPTPSDTPTQTPTPLSIIRDNADASTIVSSQWTLMSSQDGQYGANYLLADKGNGSET
                     VRYRFAVEKSGRYGLYEWHPWIYNSVYDAALTIQHAAGQSELIVNQRAPERAGKWNYL
                     ASFDFSVGTYDLVMSNKAGNGIVVVSDAFMLQIEED"
BASE COUNT          300 a          371 c          447 g          343 t
ORIGIN      
        1 aggcggagat tgactacttc ctggagaagg ttgcggaagg gtatccagtc caacgcgcga
       61 ctgccaagga ctcccgcctg gctgttgagg tctgtcttgc tgagatggaa tctgcaagaa
      121 aaagaacaac cgtcgtgctg taagagtccg cccgaatcag gtattcgcct gccttcagtc
      181 ttcttcgatc tggagcatga aagcatcgga gacaacgaca atgccgttgc ccgccttgtt
      241 gctcatgaca agatcgtacg taccgacaga gaaatcgaaa gaggcgagat agttccactt
      301 tcccgctcgc tcaggtgccc tctggttgac gatcaactcc gactgaccgg cggcatgttg
      361 aatggtcaat gccgcatcat agacgctgtt gtagatccac ggatgccatt catagagccc
      421 gtacctgccc gacttctcga cagcaaaacg gtagcgcacc gtttcgctgc cgttgccttt
      481 gtcagcgaga agatagttgg caccatactg cccgtcctgg ctgctcatga gcgtccactg
      541 tgaagataca atggtgctgg cgtccgcgtt gtcccgaatg atactcagag gcgtcggcgt
      601 ctgcgttgga gtatcggacg gtgttggagt caccgtcgga gtgttgctgg ccgttggtgt
      661 tcgtgtcggg gtcatggtga gagtgaatgt tgctgtcggt gtgacggttg gcgtctgcgt
      721 tggtgaatcg gagggagtca tggtcggagt ccaggttggc gtgtcggtct gagtcggagt
      781 gtctgtcgag gttgtggtcg gtgtgaccgt tgccgacggc gtgatcgttg gagtgtttgt
      841 caccgtcgct gttggcgttg tcgttggggt ctgcgttggc gtccgcgcag ggagaggtct
      901 acttgagtgc caacccccga taaacgccag aatgtcatcc agattcagaa tgccgtctcc
      961 cgtaaagtct atcagggagt ttggattgcc aatctcgccg gcaaggacaa agagttccga
     1021 agcgtccaat ctgttgtctg gagtgacaac gcaataagcc tggagaggct tggagagatt
     1081 gccgacaaca tcactggcca tcacggtgaa tgtccatgtg cccgtttctg agaagagaat
     1141 cgaaggactg cggaacatgt cttgggtggc attcaacaga agcagctgga cttcggactc
     1201 acacgacgtc attcccgtca tgtaggccgc tgtcacatca gccacttcag actgatccat
     1261 gatctgagca gtcagtgcag caccggaaag gtatttcgcc gattgcggga cgtcgagatc
     1321 caagatagtc ggtggtgtca agtcggtgtc tgggtcaccc tgcgccgtgc cgaaaacaat
     1381 acgcgacgca tcgctgttga ccgctttgat ctgcggagtc tgcgcggtca aacccgccat
     1441 cagctgcaac tctgtggccg c
//
LOCUS       DAOQUC010001203         1460 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_121844_length_1460_cov_3.595730, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001203 DAOQUC010000000
VERSION     DAOQUC010001203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1460)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1460)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1460
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121844_length_1460_cov_3.595730"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1460)
                     /locus_tag="PKH07_16905"
     CDS             complement(<1..>1460)
                     /locus_tag="PKH07_16905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015942422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HNT36669.1"
                     /translation="SRWALLTDREIFRRYKRIRAPRHAGLGIPVRDVLDLRPEDYVVH
                     EEHGIAQFVRLARLKVDEKEGEFIELRFADQGKLYVPVDNVHLIGRYVAGSEQPPKLS
                     KLGSKVWENTKRRAQEAIEEMSGELLELYARRSVAQGMAFSKDSPWQREFEASFVYNE
                     TPDQLRSIEEVKKDMESPKPMDRLLCGDVGFGKTEVALRAAFKSVMEGKQVAVLVPTT
                     ILAQQHYRTFAERMADYPIEIRDLSRFRSAAEQKETVRELAQAKADIVIGTHRLLSSD
                     VKFRDLGLLIVDEEQRFGVKHKERLKQMKTSVDVLTLTATPIPRTLYLSLSGLRDMSQ
                     LSVPPEDRLPVLTYVVDFKAPVIESAILREMARGGQIYFLHNRVQTIGSMANYVQKLV
                     PDARIAVAHGQMPEKQLERLMMEFVEGQYDVLVCTTIIESGLDIPNVNTILINRADMF
                     GLADLYQLRGRVGRERHQAYCYLLVPGTKAITPQAR"
BASE COUNT          328 a          423 c          360 g          349 t
ORIGIN      
        1 tcccgcgcct gcggggtgat cgccttggtg cccggcacca gcaaatagca ataagcctgg
       61 tgtcgctctc gaccgacacg ccctcgcagt tgataaagat ccgccagtcc gaacatgtct
      121 gcccggttga tcagaatcgt gttcacattg ggaatgtcca gacccgattc gatgattgtc
      181 gtacagacca gaacgtcata ctgcccctcg acaaactcca tcatcaatcg ctccagctgt
      241 ttctcgggca tctggccatg agccacggca atgcgggcat caggaaccag tttctgaacg
      301 tagttggcca tactcccaat cgtttgaacg cgattgtgca ggaaatagat ctgtccccca
      361 cgtgccatct ccctgagtat cgccgattcg ataacgggtg ccttgaaatc cacgacgtag
      421 gtcaggactg ggagccggtc ctccggcgga acgctcaact gactcatatc gcgcaaaccc
      481 gacaacgaga gatagagcgt tctcggaatg ggtgttgccg taagagtcag aacatccact
      541 gacgttttca tctgtttcag acgttccttg tgcttgacgc cgaaacgctg ctcttcatca
      601 acgataagca aaccgaggtc tcggaatttg acgtcggaag acaaaagccg atgcgttccg
      661 atgacaatgt ccgccttggc ttgtgcgagt tccctgaccg tttctttctg ctcggcggcg
      721 gaacggaaac gggacaaatc ccgaatttct atgggatagt ccgccattct ctcagcaaaa
      781 gttcggtagt gctgctgagc gagaatcgtt gttggcacca gaaccgcaac ctgtttgcct
      841 tccatcaccg acttgaacgc cgctcgaagt gccacttcgg tttttccaaa accgacatcg
      901 ccgcacaaaa gacgatccat cggcttgggc gattccatat ctttcttgac ttcttcgatg
      961 ctgcggagtt gatccggtgt ttcgttgtag acaaaggacg cttcaaattc acgctgccac
     1021 ggtgaatcct ttgaaaacgc cataccttgg gcaacgcttc gacgggcata caactccaga
     1081 agttccccag acatttcttc aatggcttcc tgggcgcgtc gtttggtatt ttcccagact
     1141 ttgctgccaa gttttgagag tttgggtggt tgctcggaac cggccacata tcggccaatc
     1201 agatgaacgt tgtccacagg cacgtagagc ttaccctgat cggcaaaccg aagttcgatg
     1261 aactctcctt ccttctcgtc caccttcaaa cgagcaagcc gcacaaactg cgcgatgccg
     1321 tgttcctcat gcaccacata gtcttcggga cgcaagtcca gcacatctcg aacaggaatc
     1381 cccagacccg catgtcgtgg tgcgcggatt cttttgtacc gtctgaagat ttcccgatct
     1441 gtgaggagtg cccaccggct
//
LOCUS       DAOQUC010001204         1459 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_122009_length_1459_cov_2.937322, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001204 DAOQUC010000000
VERSION     DAOQUC010001204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1459
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122009_length_1459_cov_2.937322"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1114)
                     /locus_tag="PKH07_16910"
     CDS             complement(<1..1114)
                     /locus_tag="PKH07_16910"
                     /inference="COORDINATES: protein motif:HMM:NF024589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNT36670.1"
                     /translation="MASGAVCPFCNATIEPEFPLCSSCGALLVGASVGVTHFDSTAGL
                     LPFVGRTDEMESIRRAAETALAGSKQILWISGRPGVGKTRLLSEAVSTLLSQFHVTRS
                     VCSSHGQYVPFRAFADLFRKAADIRLNDSVENNLKQIQKRLVVPEGFEVQHWDQRLVE
                     LLGWQLGNGQGIPATERRRQTILSAKTALLGLARTRPTCLILEDIQWMDASSWELLDG
                     ILNDFEDLPLLICLTSREPWQPARELPHECLTKIVLQPLDSEQTSDLLGAIFASHTLP
                     DFMLDSLKKDQEWTPLYIDQLVRKLISQNIVKEGKDDWMQETPLDDELIPRELVNLIQ
                     SRIAGLGEGIRQILEVGAVIGAPFTSDLLERLQICSE"
     gene            complement(1126..1314)
                     /locus_tag="PKH07_16915"
     CDS             complement(1126..1314)
                     /locus_tag="PKH07_16915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008933269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNT36671.1"
                     /translation="MDEKLMTLDNLAEYLNLSRRTVYRLLQEKDLPAYRIGSHLRFRR
                     GDIDDWLQTKRLDDSKLG"
BASE COUNT          360 a          398 c          375 g          326 t
ORIGIN      
        1 cttcggaaca tatctgcagg cgttccagca aatcggacgt gaacggagcc ccaatgacag
       61 cgcccacctc aagaatctga cgaatgcctt ccccaagtcc ggcaatacgc gattgaatca
      121 agttgacgag ttctcgtgga atcagttcat cgtccagagg cgtttcctgc atccagtcat
      181 cttttccctc tttgacgatg ttctgactga tcagttttcg caccaactga tctatgtaga
      241 ggggtgtcca ctcctgatcc tttttcaagg aatcgagcat gaaatcaggg agtgtgtgag
      301 aggcgaagat tgctccgagc aagtctgaag tctgttctga atccaatggc tgaagaacga
      361 tctttgtcag gcattcgtgt ggaagttccc tggcgggttg ccaaggttct ctggaagtca
      421 gacagatcag gagaggcaga tcttcaaagt cattcagaat tccatcgagc agttcccaac
      481 tcgatgcatc catccactga atgtcttcca ggatcaagca ggtcggtctg gttcttgcaa
      541 gaccgagcag agcagtcttg gcggacagga ttgtctgtcg cctgcgttcg gtggcaggaa
      601 tgccctgtcc gtttcccaac tgccaaccca ggagttccac aagccgttga tcccagtgtt
      661 ggacttcgaa tccctcagga acgacgagcc ttttctgtat ctgcttaagg ttgttctcga
      721 cggaatcgtt gagccgaatg tccgctgcct tgcggaacag gtcagcgaat gcacggaatg
      781 ggacgtattg accatgagag gaacaaacag atctcgttac atggaattgg ctcagaagag
      841 tggacacggc ttcggaaagc agtcgggtct ttcccacgcc aggcctgccg gagatccaga
      901 gaatctgctt ggaaccggca agagccgttt ccgcggcacg acgaatgctc tccatctcat
      961 cagtgcggcc gacaaaaggc aacagccccg cggtggagtc aaagtgagtc acgcccacag
     1021 aggctccgac caacaaagca ccgcaggatg agcacaaagg aaactctggc tcgatcgttg
     1081 cattgcagaa cggacaaaca gcacctgatg ccacccaaac agctcctagc ccagtttaga
     1141 atcgtccaac ctcttggtct gcagccaatc gtcaatgtcg ccccgcctga acctcagatg
     1201 actcccaatg cggtaggcgg gtaaatcctt ctcctgaagc aggcgataga ccgttcgccg
     1261 actcaggttc aggtactcgg caagattgtc caacgtcatg agtttctcgt ccataccatc
     1321 tgcctagccc agaccatctt ttgcgggcat ataatggcac acggtggcac actttgtcaa
     1381 cagagagata ccgttttcat tgggcgtgct gaacttgaga gcactgtcga cagctgcaca
     1441 catacagaac aggcaaaac
//
LOCUS       DAOQUC010001205         1459 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_122026_length_1459_cov_2.695869, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001205 DAOQUC010000000
VERSION     DAOQUC010001205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1459
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122026_length_1459_cov_2.695869"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="PKH07_16920"
     CDS             <1..309
                     /locus_tag="PKH07_16920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36672.1"
                     /translation="DMSEFLIRWAERPTPIPNSSIREFLGWVETGLMTEDRGYPSHSR
                     RFIEDCIGIMEGQINDMNLLIAQSVISGLRIAGTRFQRSRSERRALLRDALNNNFPEE
                     "
     gene            complement(422..>813)
                     /locus_tag="PKH07_16925"
     CDS             complement(422..>813)
                     /locus_tag="PKH07_16925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36673.1"
                     /translation="SGYSKSGSWTTVTAGGTEPLNGDYDYTATTTGAATAWARWTPNL
                     AASGNYTVSVRYRVISKAAADSPYTVYYNGGNQTVDVNQTVNGGTWVLLGTFNFSAGT
                     GGYVQLNNGPAQGKKNVCADAVKFELQ"
     assembly_gap    814..823
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<824..>1459)
                     /locus_tag="PKH07_16930"
     CDS             complement(<824..>1459)
                     /locus_tag="PKH07_16930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36674.1"
                     /translation="NTPDAQATNTPTNTPGGDVVVDNSDGGFSILSGSWSLGTSSTDK
                     YGVDYRYNTCGTGADVVQWSAPLVTGTYSVYAWWPQGTNRAADAPYTIYYAGGSQTID
                     VNQQVNGGNWNLLGTYSFTAGDWNVRLSDDGIDTAKVVMADAIKWVRQSDPVTNTPTN
                     TPSNTPTNTPTVLTNTPTDTPTDSGPTDTPTDILTNTPTNTPTDTPVAIII"
BASE COUNT          302 a          373 c          410 g          364 t
ORIGIN      
        1 gacatgagcg agttcctgat tcgctgggca gagcgtccga cgccaatccc gaactcatcg
       61 atccgggagt tcctcggctg ggtcgagacg ggcctgatga cggaagatcg aggctatccc
      121 agtcacagtc ggcggttcat tgaagactgt attggcatca tggaaggcca gatcaacgat
      181 atgaatcttc tcatcgctca gtccgtcatc tcgggactcc gaatcgcggg aacccgtttt
      241 caaaggtctc gttctgagcg tcgagccttg ctgcgggatg ctctcaacaa caatttccct
      301 gaagaatgaa tccagtttcc ctcacagaaa agtagtgcca gcgattgctt acgataccaa
      361 agagaaggga gcagaacgca ctgcatcctg ctccctgaag tgtgaaagcc tttcgtagtg
      421 attactgcaa ctcgaatttt acggcatctg cacagacgtt cttcttgccc tgtgctggcc
      481 cgttgttcag ttggacgtag ccaccagtgc cagcgctgaa gttgaatgtt cccaggagaa
      541 cccaggttcc cccattgacg gtctggttca catcaacggt ctgattgcca ccgttgtagt
      601 agaccgtata gggagaatcg gctgccgcct tggagatgac tcgataccga acagagacgg
      661 tgtagtttcc tgacgccgcc agattcggtg tccaacgtgc ccatgcggtt gccgctcctg
      721 ttgttgttgc cgtgtagtcg taatccccat tgagtggctc cgttccgcca gcggttacgg
      781 ttgtccacga acctgacttt gagtatccgg agtnnnnnnn nnncaataat gatggcaaca
      841 ggcgtatctg tcggggtgtt ggtcggtgtg tttgtcagaa tgtcggtagg tgtatctgtc
      901 ggtccgctgt cggtgggagt atcggtcgga gtgttcgtga gaacagtcgg cgtattcgtt
      961 ggggtattcg atggagtatt ggtcggagtg ttggtgacgg gatcagactg acggacccac
     1021 ttgatcgcgt ccgccatcac cacctttgct gtgtcaatgc catcatctga aagtcggacg
     1081 ttccagtcac ctgctgtaaa actataggtt ccgagcagat tccaattgcc accgttgacc
     1141 tgctggttga cgtctatcgt ctgcgatcca ccagcgtaat agatcgtata gggcgcgtca
     1201 gctgcgcggt tggtaccctg aggccaccag gcatagacgc tgtaggttcc cgtgacgaga
     1261 ggggctgacc attggaccac atcagctcca gtaccacaag tgttgtagcg atagtccact
     1321 ccatatttgt ccgtggaact cgtgcccagt gaccaggaac cacttagaat cgagaaacca
     1381 ccgtcgctat tgtccacaac gacatcgcca ccaggtgtgt tggttggggt atttgttgct
     1441 tgtgcgtcag gagtgtttg
//
LOCUS       DAOQUC010001206         1459 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_122031_length_1459_cov_2.648860, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001206 DAOQUC010000000
VERSION     DAOQUC010001206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1459
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122031_length_1459_cov_2.648860"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..513
                     /locus_tag="PKH07_16935"
     CDS             <1..513
                     /locus_tag="PKH07_16935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNT36675.1"
                     /translation="QENGGLALTTGQVGTAYGIVGLICLSVGGILGGFMAAKHGLKKW
                     IWWMALAINVPDAVYVYLSRVQPDNLFIINLCVGIEQFGYGFGFTAYMLYMIMVAEGK
                     HKTVHFAIATAFMAAGMMIPGMLSGWVQECIGYKAFFVWVLIATIPGFLMLLFIPLDA
                     EFGKKEKKAA"
     gene            540..>1459
                     /locus_tag="PKH07_16940"
     CDS             540..>1459
                     /locus_tag="PKH07_16940"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HNT36676.1"
                     /translation="MISKRALVCVTVVMVGCLCGCSEYRVQKKVTMEQWATWDRGPLV
                     IPLYAKNLKGLKICLDPGHGGDAHLKGYKAGPKTGVREAAVNWRVAVFLRQLLEEAGA
                     EVIMTRYGDEDVSLSRRVQIANENNADIFVSLHHNATGSDKVNYTTTWFHADPDSAPA
                     NLDLARQIQIGVEEELRLPQTHPRGLMSDYLMYPGAGFGVIRGLNCVACLCEASFHSH
                     AEEEKRLANAWYNQREAYGYFLGIARYVNGGMPFFRMTEPRDGDRVETGTPTLKFRIN
                     DGLSARGGWAADRNWILEESIQVSVNGKKVP"
BASE COUNT          328 a          328 c          464 g          339 t
ORIGIN      
        1 caggagaacg ggggcctggc attgacgacg ggacaggtgg gaactgccta cggtattgtc
       61 ggtctgattt gtctttccgt gggtggaatt cttggcggct ttatggcagc caagcacgga
      121 ctaaagaaat ggatctggtg gatggctttg gcgatcaatg ttcccgatgc ggtctatgtt
      181 tatctgtcac gggttcaacc ggacaatctg ttcatcatca acctgtgtgt tggtattgag
      241 cagttcggtt acggctttgg tttcacggcc tatatgctct atatgatcat ggttgctgag
      301 gggaagcaca agaccgtcca ctttgccatt gcaaccgcat ttatggcggc ggggatgatg
      361 attcctggaa tgctcagcgg gtgggttcag gagtgtattg gatacaaggc gttctttgtc
      421 tgggtcttga tcgcaaccat tccgggattc ctgatgctgc tgttcatccc tctggatgca
      481 gagttcggca agaaagagaa gaaggcggcg tagcggcggc ggttgaaagg atgcgcaaga
      541 tgataagcaa gagagcattg gtctgtgtga cggtcgtgat ggtgggttgc ctttgtggtt
      601 gcagtgagta ccgcgtccaa aagaaggtca cgatggagca gtgggcgacc tgggacagag
      661 gcccgctggt aatcccgctc tacgccaaaa acctgaaagg cctgaagatt tgtctggatc
      721 cgggtcatgg tggagacgcg cacctgaaag ggtataaggc aggccccaag acgggagttc
      781 gggaggcggc ggtcaactgg cgtgtggcgg tgtttctgcg tcagctgctt gaggaagcgg
      841 gtgcagaagt gatcatgacg cgctatggag acgaggatgt cagcctgagc cgaagagtgc
      901 agatcgccaa tgagaacaac gccgacatct ttgtgtctct gcaccacaac gcgaccggca
      961 gcgataaggt caactatacg acaacctggt ttcatgccga tccggattct gcccctgcaa
     1021 atctcgatct ggcgagacag attcagattg gggttgagga agaactgcga cttccgcaaa
     1081 cccatccgcg aggcttgatg tccgactatc tgatgtatcc aggagccggt ttcggcgtca
     1141 tcagagggct caattgcgtg gcctgtctct gtgaagcgtc ctttcattct catgcggaag
     1201 aggaaaagcg gctggccaac gcctggtaca atcagcgcga agcctatgga tacttcctgg
     1261 gtattgcccg gtatgtcaac ggtggaatgc cgttcttccg catgacagaa ccacgagatg
     1321 gtgatcgagt cgagaccgga acgccaactt tgaaatttcg aatcaatgat ggtttgtcgg
     1381 cacgaggcgg ctgggcggca gatcgaaatt ggattctcga agagagcata caggtcagcg
     1441 tgaatggcaa gaaggtgcc
//
LOCUS       DAOQUC010001207         1457 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_122326_length_1457_cov_2.328103, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001207 DAOQUC010000000
VERSION     DAOQUC010001207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1457
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122326_length_1457_cov_2.328103"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="PKH07_16945"
     CDS             <1..198
                     /locus_tag="PKH07_16945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013033196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HNT36677.1"
                     /translation="VLRARKLQRYLTQPFHVTAELTGIAGAAVPLADTLRDCEAFLRG
                     LYDGLPEDHCYMRGSMAEATA"
     gene            195..602
                     /locus_tag="PKH07_16950"
     CDS             195..602
                     /locus_tag="PKH07_16950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008937276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HNT36678.1"
                     /translation="MKPFTLLVQDATHLERFDDVGHFIGWDDSGAFGLLAGHERFMTI
                     LSWGLARFQTMDERWQFLALPGGLLYVRQNLVTISTRRFFRDDDVDRISRRLEDELSV
                     EEEGLRSIKESLSRLEEEMFKRFWKMGRGEWAS"
     gene            599..871
                     /locus_tag="PKH07_16955"
     CDS             599..871
                     /locus_tag="PKH07_16955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008937275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HNT36679.1"
                     /translation="MSLADNKLKERISRQTRRMKQAERDRPTLLGQTVYVGTLGLLMV
                     IPIVVGAYVGQWLDSLVEGYSIRWTIGLILIGVVIGAVNIYLFVKE"
     gene            875..>1457
                     /locus_tag="PKH07_16960"
     CDS             875..>1457
                     /locus_tag="PKH07_16960"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HNT36680.1"
                     /translation="MEVNPVFSLGPIGVTVPVVTTWGIMLALVTLSWLATRSLQQKPG
                     PTQVVLEGVIGAMDRAVREVLPNAATQVLPFIGTLWVFVVVANLVGIIPGLHSPTGAL
                     STTAALAVLVFISVHWFGIRSEGMSAYLKHYLAPNPIMLPFHLMSEVTRTIALAIRLF
                     GNIMSLEMTAMLVLLVAGFLVPVPLLMLHVIEAL"
BASE COUNT          279 a          360 c          460 g          358 t
ORIGIN      
        1 gtgcttcgag ccaggaagct gcaacggtat ctgacgcagc cgtttcatgt tacggctgag
       61 ttgacgggca tcgcgggagc tgcggttcca ttggcagaca ccttgcgaga ttgtgaggcg
      121 ttcctgcgtg gtctctacga cggtctgccc gaggatcatt gttacatgcg tggatcgatg
      181 gcggaggcga ctgcatgaaa ccgttcaccc tgctcgttca ggacgccact catcttgaac
      241 gattcgacga tgtcgggcac ttcatcggat gggacgattc aggtgcgttc ggcctgttgg
      301 ccggtcacga acggttcatg accattctgt cttggggact cgcacgattt cagacgatgg
      361 acgagcgctg gcaatttctc gcgctccctg gtgggcttct gtacgttcgg cagaatcttg
      421 tgacgatcag cacgcgtcgg ttttttcggg acgatgatgt ggaccggatc agtcgcaggt
      481 tggaggatga gttgtcggtt gaagaggaag gcttacgatc gatcaaggaa agcctcagcc
      541 gcttagagga agaaatgttc aaacggttct ggaagatggg acggggagag tgggcatcgt
      601 gagtttggcc gacaataagc tcaaggaacg catcagtcgg cagactcgtc ggatgaagca
      661 agccgaacgg gaccgcccga ccctactggg acaaactgtc tatgtgggaa cacttgggtt
      721 gctgatggtc atccccattg tggtcggcgc ctatgtcgga caatggctgg acagtcttgt
      781 ggaaggatat tcgatacggt ggacgatcgg cctcattctg atcggagtgg tgatcggggc
      841 ggtgaatatc tacctgttcg tgaaagagtg accgatggaa gtgaatcctg tcttctctct
      901 tggtccgatc ggggtgaccg taccagtggt gaccacctgg gggatcatgc tcgccctcgt
      961 gacgctgtca tggttggcga ccagatctct tcagcaaaag ccgggaccga cgcaagtcgt
     1021 tcttgagggt gtgatcggcg caatggatcg tgccgttcgg gaggtgttgc cgaatgctgc
     1081 gacgcaagtg ctgccgttca tcggtaccct ctgggtcttc gtggtcgtgg ccaatcttgt
     1141 cggtatcatt ccaggccttc attcgccgac gggagcgttg tccacgacgg cggctctggc
     1201 ggtccttgta ttcatctcgg tccattggtt cggcatccga tcggaaggca tgagcgccta
     1261 tctcaaacac tacctggcac cgaaccccat catgttgccg tttcacctca tgagcgaggt
     1321 cacgagaact attgcgttgg ccattcggct cttcggaaac atcatgagtc tggaaatgac
     1381 ggcgatgctg gtgttgctgg ttgctggctt tctggttccg gtgccgcttt tgatgttgca
     1441 cgtaatcgaa gcactga
//
LOCUS       DAOQUC010001208         1456 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_122434_length_1456_cov_2.936474, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001208 DAOQUC010000000
VERSION     DAOQUC010001208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1456
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122434_length_1456_cov_2.936474"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1456
                     /locus_tag="PKH07_16965"
     CDS             <1..>1456
                     /locus_tag="PKH07_16965"
                     /inference="COORDINATES: protein motif:HMM:NF023400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HNT36681.1"
                     /translation="TTRFSAPLTFRKNPKAGSVILGRFRDNASGSDVRTPFRIFRISD
                     LGISCKRSEKDTLIWVTSLRTGKPQADVSILAFDRHGFGYPLGKTNKQGVLYVADGSQ
                     ATRIDLRGTQERIVVEETALQAGDLTWVVAFSESDISYVWLQVEWLPVPSEFTENTSD
                     IKAVLFTERGVYRPGEKVFFKGTVRQWEEETVGLPQNATCSIEIVNPVGTALYNSSQR
                     YSKFGTVHGELEIPSYAKLGTYQIVLRNSKQVELTRRAFNVEEFRQPRHTVEILFSRE
                     TRTSTEYINLDREQHLLECRISSRYLAGGPVKHGKVRWKVSAVATDYPQSKHPTFQFG
                     HPTESDSDDFLETGESILDEQGEAVVRLPLGRAALGGVHGIKVDATVVDFDGRAATGK
                     GVYQVEPKYLVGLSEHPETVRAGDAQVLRVLVLNEKRLPVKKGSVQITVYQQGYYYHR
                     MRNQEGNMYWKWNEIWRQQYSADLTLLNGLAQFD"
BASE COUNT          338 a          368 c          426 g          324 t
ORIGIN      
        1 agaccacccg attctccgcc ccactgacgt tccgaaagaa tccgaaggcg ggcagcgtga
       61 tactgggtag attccgagac aacgcatcgg gcagcgacgt ccgtactccc tttcggatat
      121 tccgcatctc cgatctgggc atcagctgta aacgatcgga gaaggacact ttgatctggg
      181 tgacatcgct cagaacgggc aaacctcagg cggacgtctc cattctggct tttgatcgcc
      241 atggctttgg ttacccgctt ggaaagacca acaaacaagg cgttctgtat gtcgcggacg
      301 gcagtcaggc aacgcgaatt gacctgcgtg gcacgcaaga gcgcattgtc gttgaagaaa
      361 cggcgctgca ggcaggtgat ctgacttggg ttgttgcctt tagtgaatca gacatctctt
      421 atgtctggct acaagtggag tggctgcctg tcccatccga gttcaccgaa aatacttccg
      481 atatcaaggc agtgctcttt acggaaagag gggtctaccg tcctggggag aaggtctttt
      541 tcaagggcac tgttcgccag tgggaagaag aaaccgtcgg acttccccag aatgcgacct
      601 gttccatcga aattgtcaat cccgtaggga ctgcgctcta caactcttcc cagcgctact
      661 cgaagttcgg aactgtgcat ggcgaacttg agattccttc ctatgcgaag ttgggaacct
      721 atcagatcgt tctccgcaat tccaaacaag tggaactgac gaggagagcg ttcaatgttg
      781 aggaattccg tcagcctcgc cataccgttg aaattctctt cagccgggaa actcgaacgt
      841 ccacagagta tatcaacctg gacagggagc agcatctgct ggagtgcagg atctccagtc
      901 ggtacctggc agggggccct gtcaagcatg ggaaggtgcg ttggaaggtt tccgccgtcg
      961 ccaccgatta cccgcagagc aagcatccga cgtttcagtt tggtcatccg acggaatcgg
     1021 actccgacga cttcctcgag acgggtgaat ccattctgga tgaacaggga gaggcggttg
     1081 tgagactccc cttggggcga gctgccttgg gtggtgtcca cggcatcaag gtagatgcaa
     1141 cagtcgtgga ttttgacgga cgagccgcga caggcaaggg agtctatcaa gttgagccga
     1201 agtaccttgt cgggctgagc gagcatccgg agacggtgcg ggcaggcgac gcgcaggtgt
     1261 tgagggttct tgtcttgaac gagaaacgcc tgccagtgaa gaaagggtct gttcagatta
     1321 ccgtctatca gcagggttat tactaccatc gaatgcgcaa ccaggaaggg aacatgtact
     1381 ggaagtggaa tgagatctgg cggcagcagt attccgcaga cctgactctg ctgaatggtc
     1441 tggctcagtt tgattt
//
LOCUS       DAOQUC010001209         1455 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_122592_length_1455_cov_2.425000, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001209 DAOQUC010000000
VERSION     DAOQUC010001209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1455
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122592_length_1455_cov_2.425000"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            329..>1455
                     /locus_tag="PKH07_16970"
     CDS             329..>1455
                     /locus_tag="PKH07_16970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HNT36682.1"
                     /translation="MKKCFVYLSFVCCIAIVLAIAGCGQKPESKPGTAAPKQKAQQPA
                     QPVQEEPEAVEAPAAAEAEPAAEQPVDPKDVINLSYSIFFPEAHIQCKTAMAWAEEIG
                     KRTNGKVKITVYPGGSLTQAPQCYEGVMNNISDIGMSCFSYTRGRFPLLEGLDLPLGY
                     PDGIIATRIATQMVAKYMPAEVADVHVLYVHAHGPGILASKKPVRSLADMKGMKVRVT
                     GLSSKIVESLGGVPVAMPQPDTYEALQKGVVESTLCPIETLKGWKQGEVVNFITDSSA
                     IGYTTAMFVVMNLSKWNALPAEIQDIFNQVSSEWVDQHGQAWAQADQEGEAFVKELGH
                     ETISLDEAEVANWKQSVQPILDEYVAAAKEKGLPGEALLND"
BASE COUNT          368 a          353 c          423 g          311 t
ORIGIN      
        1 gcaagcgtcc gaaggagtgg gcatagccca cgtgcctgca ggcgttggcc tgctcagaag
       61 gatttacttt cctgacggga tatgttctaa tatgaatatg tttctagagg gatttggagc
      121 cgtcagagca ccaacgccct gaagacggaa aggagggagt aatctttagg gtaacagcca
      181 agacgctctg aacgtcgtcc ttcacgtggc agggatgaat ggacgcattc ataagagctc
      241 agagcgatgg cagaccgaga tgcagggctt ggctcggaag agagtggatc aagacaagtt
      301 ttaggggatt tttgaaaggg tggaaatcat gaagaagtgt tttgtctatc tatcgtttgt
      361 ctgttgtatc gcaatcgtgc tggcgattgc tggctgtggc cagaaaccgg aatcgaaacc
      421 tggtaccgct gctcccaagc agaaggcaca gcaacctgca caacccgtgc aggaagagcc
      481 ggaggcagta gaggcccccg ccgccgcgga agccgaaccc gctgcggaac aacctgttga
      541 tccgaaggat gtcatcaatc tctcctacag tatattcttc ccagaggcac acatccaatg
      601 caaaacggct atggcatggg cggaagaaat cggcaagcgc accaacggca aggtgaagat
      661 tacagtctat cccggtggct cactgactca ggctcctcag tgctacgagg gagtcatgaa
      721 caacatctct gacattggga tgagttgctt ttcgtatact cgaggccgat tcccgctgtt
      781 ggaaggactc gatttgccct tgggctatcc cgatggaatc atcgccacaa gaattgccac
      841 acagatggtc gcaaagtata tgccggccga agtggcggat gtgcacgtcc tgtatgtcca
      901 tgctcacggt cctggaatac tggcttccaa gaagcctgtt cgcagtcttg ccgacatgaa
      961 gggtatgaag gttcgagtga cgggactctc atcaaagatt gttgagagcc tcgggggcgt
     1021 ccccgttgcc atgcctcaac cggacactta cgaggcgctt cagaagggag tggtcgagtc
     1081 aactctgtgc ccgatcgaaa ccctcaaggg ctggaaacaa ggcgaagttg tcaactttat
     1141 caccgactcg tctgccattg ggtacacaac ggcgatgttc gttgtgatga atctctccaa
     1201 gtggaatgcc cttccggcag aaattcagga catcttcaac caggtcagca gtgaatgggt
     1261 tgaccagcat ggtcaggctt gggcacaggc agaccaagag ggagaagctt ttgtcaaaga
     1321 actgggacac gagactatct cccttgatga agccgaggtc gcaaactgga agcaaagtgt
     1381 tcagccgatc ctggatgagt atgtggccgc tgcaaaggaa aaggggcttc ctggagaggc
     1441 tctgctgaac gacat
//
LOCUS       DAOQUC010001210         1454 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_122663_length_1454_cov_4.343102, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001210 DAOQUC010000000
VERSION     DAOQUC010001210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1454
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122663_length_1454_cov_4.343102"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..934
                     /locus_tag="PKH07_16975"
     CDS             <1..934
                     /locus_tag="PKH07_16975"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNT36683.1"
                     /translation="MPVPLLNCIPVIADSAPGCYNSGPEQIEAVITPLTGAILFAHIG
                     GEPADVEGIMAVAKKHNLPVVEDCAQSHAAKVNGKYVGTFGDIAAFSTMFGKHFCCGG
                     QGGLVFTKREDLYWSIRRSADRGKPFGLPAGSTNCLGSLNFNMDELGATMGRAQLKKL
                     PMIVEKRRRVVEALKEGFRSLKSVSAPPQVSGAEPTYWFLRTRFHKEAVTCDKMTFCR
                     ALTAEGLPVNPEYRAALPHTMDWFVNRRVFGDSQYPWSSPEYKGDAKRQFPCPNVNQS
                     MDEHFHIQVHEGWSDKEVKDALAILRKVEDAFSR"
     gene            959..>1454
                     /locus_tag="PKH07_16980"
     CDS             959..>1454
                     /locus_tag="PKH07_16980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36684.1"
                     /translation="MMIIDFHSHPDWHKHDLKKFLANMDQFHIDQSVLLSWECPKDEY
                     DPGFNAATAASDGVGPIPFSRCLSYVERAPERFILGYAPDPRRPEAIDQMSAAMDIYG
                     VKVFGEMKVRMMYDNLDALRMYRFCGQRGVPVLVHLDYEFETTRKYPRPSYWYGGDIE
                     ALERA"
BASE COUNT          325 a          378 c          406 g          345 t
ORIGIN      
        1 gatgcctgtg cccttgctga actgcattcc tgtcatcgcc gactcggctc caggttgcta
       61 caactccggg cctgagcaga tcgaggcggt aatcacacct ctgacaggtg ccatcctgtt
      121 cgcccacatt ggcggagagc ctgccgatgt cgaaggcatc atggctgtcg ccaagaagca
      181 taatcttcct gtcgttgagg actgcgctca gtcacatgcg gcaaaagtca acggcaagta
      241 cgttggcact tttggcgata ttgcggcatt ctcgaccatg tttggaaagc atttctgctg
      301 tggcggtcag ggcgggcttg tctttaccaa gcgagaggat ctttactggt ccattcggcg
      361 cagcgcagat cgcggcaagc cgtttggact gcctgcaggt tcgaccaact gcctcggttc
      421 cctgaacttc aatatggatg aactgggtgc caccatgggc agagctcagt tgaagaaact
      481 ccccatgatc gtcgaaaaga ggcgtcgtgt cgtagaggca ctcaaggaag gattccgctc
      541 gctgaagtcc gtttctgcgc cacctcaggt ctcgggtgct gagccgacct actggttctt
      601 gcgcacgcga tttcacaaag aagctgtgac ctgtgacaag atgactttct gtcgcgcgtt
      661 gactgctgaa gggcttcctg tgaaccccga ataccgtgct gcattgcccc acacgatgga
      721 ctggtttgtc aatcgtcgcg tgtttggaga cagccaatac ccctggtcat cccctgagta
      781 caaaggtgat gccaagcgac agtttccctg tccgaatgtc aaccagagca tggatgagca
      841 cttccatatt caggttcatg aaggatggag cgacaaggaa gtcaaggatg cgttggcgat
      901 tctccgcaag gtggaagacg ctttttccag ataggttccc gaaacgcagt aaggtgaaat
      961 gatgatcatt gatttccaca gtcatccgga ctggcataaa cacgatttga agaagtttct
     1021 ggcgaatatg gatcagttcc atattgacca atctgttctt ctctcatggg aatgtccgaa
     1081 ggatgagtac gaccccggct tcaatgctgc aacggccgca tccgacggcg ttgggcctat
     1141 tcctttcagc cgatgcctga gttatgtgga gcgtgcaccc gaacgattca tcctcggcta
     1201 cgctcccgat ccacgccgac cggaagccat tgaccagatg agtgccgcca tggacatcta
     1261 cggggtgaaa gtgttcggcg agatgaaagt ccgaatgatg tatgacaatc tggacgctct
     1321 cagaatgtac cgattttgcg gccagagagg cgttccggtg ttggtgcatc tggactacga
     1381 atttgaaacg acgcggaagt atcctcgacc aagttactgg tacggtggcg atattgaggc
     1441 tctggaacgt gctg
//
LOCUS       DAOQUC010001211         1454 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_122692_length_1454_cov_3.098642, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001211 DAOQUC010000000
VERSION     DAOQUC010001211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1454
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122692_length_1454_cov_3.098642"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="PKH07_16985"
     CDS             <1..324
                     /locus_tag="PKH07_16985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36685.1"
                     /translation="DSEGGKRGESSFLIAFQSLGRIRGHVRVERTDVVCDLGVESEET
                     QKLIVKSRSDLFRACEQAGLDLKQFECFIVPSDRCEAFFSPFALHPDIQEPIPERVLR
                     IDLLA"
     gene            596..>1454
                     /locus_tag="PKH07_16990"
     CDS             596..>1454
                     /locus_tag="PKH07_16990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36686.1"
                     /translation="MTKRLLFLFMLVSVLPSGQSWAQDNQTYLLRHVFAPGMSATNEI
                     SIQLNGTMKMEQAPTFPYDATVNLVMPVNVLESTEENTSRLELRVRELSNEQRLGYEV
                     QKLLVRPGWMSVDDEIVFDRRTNPGPHPFQELLGTKVFITISPRGELKDFSSLDQLAG
                     MIPNADLPSQMAHGFVVFPEQPIKVGEAWRETNEMFLATNTKPVDSKTEYVLLRVDPN
                     DRGEPVAIIGVKRSAESDKVVIDGKLPSQPNPEPGQINVRKLVVKKMKQSFQGVIYFD
                     IKKGRVLRTE"
BASE COUNT          348 a          378 c          395 g          333 t
ORIGIN      
        1 gattcggagg ggggcaagcg aggcgagtcc tcttttctca tcgcatttca gtctctcggc
       61 cggattcgcg gacatgtccg ggtggagcga accgacgtgg tctgcgatct tggggttgaa
      121 tctgaagaaa cacagaagtt gatcgtcaag agccggtcgg atcttttcag agcctgtgaa
      181 caagccggtc tcgatctcaa gcagtttgag tgcttcatcg ttccgagcga ccgatgcgag
      241 gccttcttct cgccctttgc tctgcatcct gacattcagg aacccatacc cgagagggtt
      301 cttagaattg atctcctggc gtgatctcga gcgccgcctc ctgtgcctct atccacattg
      361 aaggctgcca aacttcactg acctcgtgaa ccggactcag acacgacagc tgcatcacgg
      421 cgagatcggg cgacttatcc gcacgaaaga cgattctctt gccaattatc agccaaaacg
      481 gcatattaca cacaaggcat ctggtacccg gacgtatcgg ttgcctgtta cgagagatgc
      541 ttgttttctt gcggagtcca gttgccttga gaacaggccg gtacggagta ttcggatgac
      601 aaaacgcctc ttgttcctat tcatgttggt gagcgttctt ccgtcggggc agtcttgggc
      661 acaggacaat caaacctatc tgctgcgcca tgtctttgct ccagggatgt ctgcgaccaa
      721 tgagatcagc attcagttga acgggacgat gaaaatggag caggctccaa cgttccccta
      781 tgacgcaacg gtcaatctgg tcatgcctgt gaacgttcta gagagtaccg aggagaacac
      841 ctcaagactg gaactgcgcg ttcgggagct ctcaaatgaa cagcgcttgg gctacgaagt
      901 gcagaaactt ctggttcgtc cgggttggat gtcggtggac gatgagatcg tttttgacag
      961 acgtaccaat ccaggccccc atccattcca ggaactgttg ggaaccaaag tgttcatcac
     1021 tatttcgccc cgaggagagc tgaaggattt ctcgtcgttg gatcagcttg ccggaatgat
     1081 ccccaatgcg gatctaccaa gccagatggc acacggattt gtcgtttttc ccgaacagcc
     1141 gatcaaagtg ggtgaggcgt ggagagagac caacgagatg tttctcgcta ccaacaccaa
     1201 gccggtggac tccaaaacag agtatgtcct cttgcgagtg gatccaaatg acagaggaga
     1261 acctgtcgcc atcatcggcg ttaagcgttc agcggaatcg gacaaggtcg tgattgacgg
     1321 caaactgccg agccagccta atccagaacc cggacagatc aacgtccgaa aactcgtcgt
     1381 gaaaaagatg aagcagagtt ttcagggagt gatctacttc gacatcaaga agggtcgtgt
     1441 ccttcgcaca gagc
//
LOCUS       DAOQUC010001212         1453 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_122837_length_1453_cov_3.226753, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001212 DAOQUC010000000
VERSION     DAOQUC010001212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1453
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122837_length_1453_cov_3.226753"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..757)
                     /locus_tag="PKH07_16995"
     CDS             complement(<1..757)
                     /locus_tag="PKH07_16995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydrofolate dehydrogenase/cyclohydrolase
                     catalytic domain-containing protein"
                     /protein_id="HNT36687.1"
                     /translation="MTAKIIDGKAIAEQMRLELKDEIAILRDKGIVPGLGVILVGEDP
                     ASVSYVSSKEKACRDIGIFSDDNRLPADTSEQELLALVDRMNKDPKIHGILVQLPLPK
                     HIDENKVLLAISPEKDVDGFHPINVGKMVVGEDAFLPCTPHGVIQLLLRSKVKISGAH
                     VVVVGRSNIVGKPVANMLVQKKDTGNATVTICHTRTQDISYFTKQADILIAAAGKPNM
                     ITADMVKDGVVVIDVGVNRVDDPTAKKGYRLVGD"
     gene            complement(832..>1453)
                     /locus_tag="PKH07_17000"
     CDS             complement(832..>1453)
                     /locus_tag="PKH07_17000"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010936446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HNT36688.1"
                     /translation="LLEKGCQNLTAHIETVKKSGVRPVVCINSFHTDTKAEVEAVRRI
                     AAEHGAYAAVSDHWLKGGQGAAELAETVVTACNEKNQFRFLYDEATSLTERIHRIATE
                     IYGADDVTYTETARQKVKDIESTSESRSLATCMVKTHLSLSHDPNLKGRPKGWVLPIQ
                     DILVYRGAGFVVPVAGDIKLMPGTSSDPAFRKIDVDLDTGTVTGLF"
BASE COUNT          311 a          394 c          366 g          382 t
ORIGIN      
        1 catcacccac caggcgatag cctttcttgg cggttgggtc atcaacacgg ttgacaccga
       61 cgtcaatgac gacaacacca tctttgacca tatccgcagt gatcatgttg ggctttccag
      121 cggccgcgat cagaatgtct gcctgttttg tgaaatagga gatgtcttga gttcgggtat
      181 ggcagatggt caccgtggcg ttgcctgtgt ccttcttctg gacaagcata ttcgccacgg
      241 gtttgccaac gatgttactg cgacccacga ctaccacatg ggctcctgat atcttgacct
      301 tgctgcgaag gagaagttga atcacgccgt gtggggtgca aggcagaaac gcatcctcac
      361 cgactaccat cttgccgacg ttgatcgggt gaaagccatc cacatccttt tcgggagaga
      421 tagccagcag gactttgttc tcgtcaatgt gcttcggcaa agggagctga acaagaatgc
      481 catgaatctt tggatctttg ttcatgcggt caaccaaagc cagcagttct tgttcagatg
      541 tatccgctgg aaggcgattg tcatctgaga agatgccgat gtctctgcat gccttctctt
      601 tgctggaaac ataagagacg gaggccgggt cttcgccgac aaggataact cccaagcctg
      661 gcacgattcc cttatctctg aggatggcga tctcgtcctt cagttccagg cgcatctgtt
      721 cggctattgc ctttccatca atgattttcg ctgtcatctt cactttcctc gctttgatgt
      781 ctgctgcgtt gcccaaaagg ctggtgcaaa gcacattccc ttgttcaata tctagaatag
      841 tcccgttacc gtgccagtgt ccaagtcaac gtcgatcttg cgaaaagccg gatctgagga
      901 cgtgcctggc atcagtttga tgtcgccggc aaccggaacc acaaaccctg ctcccctgta
      961 gacgagaatg tcctgaatcg ggagcaccca tcctttgggt ctccccttga gattgggatc
     1021 gtgtgaaaga ctcaagtggg ttttgaccat gcaggtcgca agactcctgc tttcagaagt
     1081 ggattcaatg tctttcactt tctgccgggc tgtttccgtg taagtaacat catctgcacc
     1141 gtagatctcg gtcgctatcc tgtgaattct ctccgtcaga gaagttgcct cgtcatacag
     1201 aaatcggaac tggttcttct cgttacaggc ggtcacaaca gtttctgcca attcggctgc
     1261 accttgaccc cctttgagcc aatggtcgga cacagccgca taggcaccgt gctcagcggc
     1321 gatgcgcctg accgcctcaa cctctgcctt ggtgtcagta tggaaggagt tgatgcagac
     1381 gacgggcctg acgccactct tcttgacggt ctcaatgtga gcagtcaaat tctggcatcc
     1441 cttttccaga agg
//
LOCUS       DAOQUC010001213         1453 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_122874_length_1453_cov_2.367668, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001213 DAOQUC010000000
VERSION     DAOQUC010001213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1453
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122874_length_1453_cov_2.367668"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..378
                     /locus_tag="PKH07_17005"
     CDS             <1..378
                     /locus_tag="PKH07_17005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012978494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNT36689.1"
                     /translation="LADTFVRRLAIATNDIEKPVGQLSGGTQQKVILARWLALEPRLL
                     ILDEPTRGIDVGAKAEIEEIVQDLCGKGMAILFISSELEEVARNAHRVVVLRDRHQIA
                     LLEGDRVSVTEIMKTIAHEGCAQ"
     gene            411..1406
                     /locus_tag="PKH07_17010"
     CDS             411..1406
                     /locus_tag="PKH07_17010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002631813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNT36690.1"
                     /translation="MVAKGHSKRLIWPIVALGMLLLLNMLFTPGFFRLEMKNGRLYGS
                     SVDILNRAAPVAIVCLGMTLVIATGGVDLSVGALMGISGSVAALLVTRTEHPLSVVVL
                     LSISLCVLAGLLSGALVSWLGVQPIVATLVLMVSGRGVAQLMTDGQIITFRHAGFEFI
                     GGGSLFCLPFTVTLVLAALLVTAVLTRKTVLGLFIEAVGDNPTASKYAGVAVHGVKVF
                     VYGFSGLCAAIAGLIVTSDIRAADASNAGLYLELDAILAVVIGGTALTGGRFSLIGSL
                     IGALIIQTLTTTILTRGVPVEHTLVVKALVVLAVCLLQSDRFRDLFVKRSRRNSP"
BASE COUNT          296 a          332 c          438 g          387 t
ORIGIN      
        1 cttgcagata cctttgtaag gagactggcc atagcgacta acgatattga gaaaccggtc
       61 ggccagctca gtggaggtac acaacagaaa gtcatcctgg ctcgctggct tgctctggag
      121 ccacggctgt tgatacttga tgagccgaca cggggtattg acgttggagc gaaagcggag
      181 atcgaagaaa tcgttcaaga cctttgtgga aagggaatgg ctattctctt tatctcatcc
      241 gagttggaag aagtggcgcg gaacgctcat cgtgttgttg tgcttcgaga cagacaccag
      301 atcgcgctat tggaggggga tcgggtcagc gtgactgaga tcatgaaaac gattgcgcac
      361 gagggttgcg cccagtaaga gaagacaggc gaaaggcaag cgttcgagcg atggttgcta
      421 agggtcacag caagagactg atctggccga ttgttgcctt ggggatgtta ctcctgctca
      481 acatgttgtt cacgcctgga ttcttccgct tggagatgaa gaacggccgt ctctacggga
      541 gctctgtgga tattctgaac cgggctgccc ccgtcgcgat cgtctgtctt ggcatgacgc
      601 ttgtcatcgc cactggtgga gtggatttgt ccgttggagc gctgatgggc atttcaggct
      661 ccgtggctgc tctgcttgtc actcgaacgg agcacccttt gtctgttgtg gtgttgcttt
      721 caatatcgct ctgcgtcctg gctggcctct tgagcggcgc gcttgtttcc tggcttggag
      781 tgcaaccaat tgttgccacg ctcgtgctta tggtttctgg acgtggcgtt gctcagttga
      841 tgacagatgg tcagatcatc acatttcgcc atgctggctt tgagttcatc ggtggaggtt
      901 ccttgttctg tcttccattt acagtgactc tggttcttgc cgcgctcttg gtcacggcag
      961 tgttgactcg aaagacggtt cttggacttt tcatcgaagc ggttggagat aaccctacgg
     1021 ccagcaaata tgccggcgta gcagtgcatg gcgtcaaagt gtttgtttat ggcttcagtg
     1081 ggctgtgcgc tgcgattgcc ggacttatcg tgacatcgga tattcgagcg gcagatgctt
     1141 caaatgcggg tctttatctt gagttggatg ccattctcgc tgttgttatc ggaggaactg
     1201 cgttgacagg agggcgattc tccttgatcg gatcgctcat aggggcgcta atcatccaga
     1261 cacttaccac gacgattctg accagaggag taccagtaga gcacacactc gttgtgaagg
     1321 ctttggttgt tctcgcagtg tgcttgcttc agtcggacag attccgggat ctctttgtga
     1381 agagatctag acggaactcg ccatagacag gaaacggttc gggagcgaag acggagaaag
     1441 atgacggtat cga
//
LOCUS       DAOQUC010001214         1450 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_123255_length_1450_cov_3.730466, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001214 DAOQUC010000000
VERSION     DAOQUC010001214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1450
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123255_length_1450_cov_3.730466"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..971)
                     /locus_tag="PKH07_17015"
     CDS             complement(<1..971)
                     /locus_tag="PKH07_17015"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HNT36691.1"
                     /translation="MLDKSVRIRNHLKSKSGLRSPMILRLRRPSNRAVLEALNMDYGD
                     KITRVLNLIQDLAARQHTKKELAEKYGVTERTILRDLDTIRRAGYELDEGTPFESGIE
                     KRYRLERRKPADQVCLTQDESILLAYCVALAAREVLPRNKTLLESVSDKIERYFPPDF
                     SQQMRRVGCLYVPLNRFRPNVEFKRDELGFLESGIVEQVWVGFRYQKSDGTSNDYVVA
                     PFAILSNAGRLYLYGWIDKHVDGEPALFDVSRIENVEILDDESDKAVKIPFPDKEIKP
                     DEIIGSSFGLWRDGPFDVEIIFSDDAAKSVQRNTFHPSQKIEMLEGN"
     gene            complement(1160..1444)
                     /locus_tag="PKH07_17020"
     CDS             complement(1160..1444)
                     /locus_tag="PKH07_17020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36692.1"
                     /translation="MADSSFVQWIYQIWDFSRIEGVDFSMDQMRGFAIQDAAAAEKNP
                     NQKIAIIPRRSDTSGLDNVFHILEDVWGGYESRTLPDVESARAWGCHASK"
BASE COUNT          318 a          398 c          359 g          375 t
ORIGIN      
        1 tgatttccct ccagcatttc gatcttctgg ctcggatgga aggtattgcg ctggactgat
       61 ttggccgcat catcgctgaa gatgatctcc acatcgaacg gaccgtcccg ccacagccca
      121 aaggacgagc cgatgatctc atcgggcttg atctctttgt ctggaaaggg gatcttcacg
      181 gccttgtccg attcgtcatc tagtatctcc acattctcga ttctggagac atcaaataac
      241 gcgggctccc cgtcaacatg cttgtcgatc caaccgtaga ggtacaaccg accggcattg
      301 gacagtattg caaatggtgc cacgacgtag tcgttggaag ttccgtcgct tttctgatag
      361 cgaaaaccga cccagacctg ttccacgatg ccgctctcca aaaaaccaag ttcgtcgcgt
      421 ttgaactcca catttggacg aaagcggttc agtggtacat agaggcaacc aacgcgcctc
      481 atctgctgtg agaagtctgg tgggaaatac cgttcgatct tgtcggaaac cgattccagt
      541 agcgtcttgt tccgtggaag cacttcccgc gccgcgagag cgacacagta agccaacagg
      601 atcgattcgt cctgggtcaa acacacttgg tctgcaggtt ttcttctctc taagcggtag
      661 cgtttctcga tgcccgattc aaagggcgtt ccttcgtcga gttcatagcc ggcgcgtcgg
      721 atggtgtcca gatccctgag gatggtgcgt tcagtcacgc catacttttc ggcgagttcc
      781 ttcttggtgt gctgcctggc tgccaggtct tggatgaggt tcagcacccg cgtgattttg
      841 tctccgtaat ccatgttgag cgcctccagt acggctcggt tcgagggtct ccgcaagcgt
      901 agaatcatcg gacttctcag ccccgatttg ctctttagat ggtttcgaat tcgaactgat
      961 ttgtcaagca tgacaactcc tgggtgtctt ggcttcacaa aacaccagga cgaaatgaga
     1021 ctgcgtcaaa cacatcctta cacagaggtc tgacgaaact tgtcatagct gggatactgg
     1081 tgaactgttg agggcaatgc ctcgcacaaa gtgatgtttc attccctcgt ccggcagagt
     1141 tccatccgaa caaggtcttc tactttgaag cgtggcaacc ccatgctctt gctgattcga
     1201 catcaggcag ggttcttgat tcgtagccgc cccaaacatc ttccaagatg tgaaaaacgt
     1261 tgtccaagcc gctcgtatcg cttctcctcg gaatgatcgc gatcttctgg ttggggttct
     1321 tctctgccgc cgccgcatcc tgaatcgcga acccccgcat ttgatccatg gagaagtcaa
     1381 cgccctcaat tcgtgagaag tcccatatct gatagatcca ttgtacgaat gaagaatctg
     1441 ccatcagtta
//
LOCUS       DAOQUC010001215         1448 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_123548_length_1448_cov_3.351759, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001215 DAOQUC010000000
VERSION     DAOQUC010001215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1448)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1448)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1448
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123548_length_1448_cov_3.351759"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="PKH07_17025"
     CDS             complement(<1..251)
                     /locus_tag="PKH07_17025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36693.1"
                     /translation="MTLRGNKMTKADYCQQQIVRILRDTLVILAISVALGCGKSGSQA
                     TGGHSTPGASTAHASGQINNESDPIPSNLPSVRVAVVEG"
     gene            complement(259..>1448)
                     /locus_tag="PKH07_17030"
     CDS             complement(259..>1448)
                     /locus_tag="PKH07_17030"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNT36694.1"
                     /translation="EISKQLITGGQIDLRYEDQRMENRLFRPDPEYYSGLNLLLTQPL
                     LDGFGRNSSLYRVRLSKKNREISSRVFEQTLLNVAYEVEAAYWDLVLAIRLVDIQKQN
                     LDLAQSLLSRNTERFDVGMLPSYEILENQSHVASRRENLLSVVNALEDANDRLATLMN
                     LLDSPAGGFSFLPLDDVVCEAFEPSASDSLAVAFERRPDLKQASLAIERSDLRLRYVR
                     NQSLPSLNLITGLGLHGFDHAYSAAHDDLRDDNTYDWRIGVVFEVPFGSRESRYQHRK
                     AVLLRQQAADQMRSLQQRVLTDIREAVRQIQTDQERIPAAMLAREFAQQRMQVEESLF
                     DQGMSTTHDLLEYQQTLALAQLDELRAQVDLRKAFSKLRFLEGRLLDEFGFEFVEPDI
                     WEE"
BASE COUNT          334 a          408 c          381 g          325 t
ORIGIN      
        1 tccccctcga caacggcaac acgcacactg ggcaagttgg atgggatggg atcggactcg
       61 ttgttgattt gcccgctggc gtgcgcggtg gatgcccccg gcgtggagtg cccccctgtt
      121 gcttgtgaac ctgatttccc gcagccgaga gcaacagaga tcgccagaat cacaagtgtg
      181 tccctcagta tgcgaacgat ctgttgttga caataatccg ctttcgtcat cttgtttcct
      241 cgcagtgtca tgttggtgtc actcttccca aatatccggc tcgacaaact cgaaaccgaa
      301 ttcgtcaagc aaacgccctt ccaaaaaccg gagttttgaa aaagccttcc gaagatccac
      361 ctgcgccctg agctcatcca gttgcgccag agccagtgtc tgctggtatt ccagcaggtc
      421 atgcgtggtg gacattccct ggtcgaagag actctcttcc acctgcattc gctgctgggc
      481 aaattcccgt gccaacattg ccgcagggat ccgttcctga tcggtctgaa tctgacggac
      541 ggcttcccga atatccgtga ggacgcgttg ttgaagcgag cgcatctgat cggctgcctg
      601 ctgccgaaga agaacggcct tcctgtgctg gtaccggctt tctcgtgagc caaagggaac
      661 ttcaaaaacc accccgattc tccaatcgta ggtattgtca tcccgcaggt catcatgagc
      721 cgcgctatat gcatgatcga aaccgtgaag cccgagcccg gtgatcagat tcagactggg
      781 aagactttgg tttcggacat atcggagtcg aaggtcggat cgctcgatcg cgaggctggc
      841 ctgtttcaga tcgggacgcc tttcgaaagc cacggcaaga ctgtcagacg cggaaggctc
      901 aaaggcttcg caaacaacat cgtcaagcgg aagaaacgag aagccacccg ctggcgaatc
      961 caggagattc atcaacgtcg caagtcgatc attagcatct tcgagcgcat tgacgacact
     1021 cagcagattc tcccttcggg aagccacgtg agactggttc tccagaatct cgtacgaggg
     1081 aagcattccc acgtcaaagc gctcggtgtt tcgactcaga agcgattgag ccagatccag
     1141 attctgtttc tggatgtcca caagtctgat ggcaagcacc aagtcccaat aggctgcctc
     1201 aacttcatag gcgacattga gcaatgtctg ctcgaaaacc cgtgaggaga tctcccgatt
     1261 cttcttgctc aagcgaactc ggtagaggga actgttgcga ccgaaaccgt ccagaagagg
     1321 ctgtgtcagc agaagattca gaccggaata gtactccgga tcgggacgaa agagtctgtt
     1381 ctccatccgc tgatcctcgt agcggagatc gatctgtcca cccgtgatca gttgcttgct
     1441 tatctccg
//
LOCUS       DAOQUC010001216         1448 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_123549_length_1448_cov_3.335966, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001216 DAOQUC010000000
VERSION     DAOQUC010001216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1448)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1448)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1448
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123549_length_1448_cov_3.335966"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="PKH07_17035"
     CDS             complement(<1..282)
                     /locus_tag="PKH07_17035"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HNT36695.1"
                     /translation="MNLVSVLSIVLALVMAPLLLGVINRTKAIFAGRHGAPLVQVYRD
                     IWKLLQKGFVYSGTTTWVFRISPLVGVACVVCAVMLIPTLGNRTLFAFQG"
     gene            complement(292..>1448)
                     /locus_tag="PKH07_17040"
     CDS             complement(292..>1448)
                     /locus_tag="PKH07_17040"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HNT36696.1"
                     /translation="GVLFALAQHDLKRLLAYHSVENIGIIALGLGIGMLGLSYGHRVM
                     ATAGFLGAILHVMNHAIFKSLLFMSVGSVLQATGTREMDHLGGLMKQMPVTAVTFLVG
                     SLAICGLPPLNGFVSEFIIYYASFKSLIGSQAIPVAGQSLCLLVIGSLALIGGLALAC
                     FTKAFGVVFLGESRSEHTAHAQETKWQMLVPMIVLALTCFAIGILSTFVLEIGLSPVH
                     IVVGQLADLHVWQADSLTDMMGFLQAIAVVASFFTFFFALLMALLFVRFRLLSKRKSA
                     KTVTWDCGYAAPTPRMQYTSSSFVQPIIQLFLPFLRTKHELRKPEGYFPESAEFSSHT
                     PDVYREHFYKPIFTAVESTLSKFHWIQHGRLNLYILSIVAALLVLLIWKL"
BASE COUNT          414 a          365 c          388 g          281 t
ORIGIN      
        1 gccctggaaa gcaaacaatg ttcgattgcc aagggtggga atgagcataa cggcacagac
       61 aacacaggca acaccgacca gagggctgat tctgaatacc caagtcgttg tgccactgta
      121 aacgaagccc ttctggagaa gtttccagat gtctcggtag acttgcacaa gcggcgcccc
      181 atgccgaccg gcaaagattg cttttgtcct gttgatgacg cccaggagaa gaggagccat
      241 caccagagca agcactatag acaagactga aaccagattc atctcttcgt cctacaattt
      301 ccagatcaga agaaccagca atgctgcgac aatggaaaga atgtatagat tcaggcggcc
      361 gtgctgaatc caatgaaact tcgacagagt ggattccacg gcagtaaaaa tcggtttgta
      421 aaagtgctca cggtacacgt caggcgtgtg actggagaat tcagccgatt cgggaaagta
      481 cccctcgggt ttcctcaact catgttttgt gcgaagaaaa ggcaggaaca gttgaataat
      541 gggttgaaca aaggatgagg aggtgtattg catccgaggt gtgggtgctg cgtagccaca
      601 gtcccatgtg accgtcttgg cgctctttcg cttggagagg agtcggaatc gcacgaacag
      661 taatgccatc agcaacgcga agaagaatgt gaagaaagag gcgacaacag caatggcctg
      721 caagaagccc atcatgtcgg tcagtgaatc cgcttgccac acatgcagat ccgccaactg
      781 cccaacaaca atgtggacag gcgacaggcc aatctccagg acaaacgtgc tcaggatacc
      841 gatggcgaaa caggtcagag caagtacaat catgggcacc agcatctgcc acttggtctc
      901 ctgcgcgtga gcagtatgtt ccgatcggct ctctcccagg aagacaacgc caaatgcctt
      961 tgtaaagcag gcaagtgcca agcctccaat cagggcaaga gatccaatga ccaagaggca
     1021 aagagactgc cctgcaactg ggatggcttg ggaaccaatg agcgatttga aagaagcgta
     1081 gtagatgatg aactcgctga cgaatccgtt cagtggcggc agtccgcata tcgccagaga
     1141 acccaccagg aaggtgacgg cagtcaccgg catctgtttc atcagtccgc caagatgatc
     1201 catttctcgg gttccggtcg cctgaagtac ggagccgaca ctcatgaaga gcagcgactt
     1261 gaaaatggcg tggttcatca catgaaggat ggctccgaga aagcctgcgg ttgccatgac
     1321 tctgtgacca tacgaaagcc caagcattcc aattcccaac ccgagtgcga tgatcccaat
     1381 gttttcaacg ctgtgatagg caagcagtct cttgagatca tgttgcgcaa gggcgaacaa
     1441 gactccca
//
LOCUS       DAOQUC010001217         1445 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_123983_length_1445_cov_2.836691, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001217 DAOQUC010000000
VERSION     DAOQUC010001217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1445
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123983_length_1445_cov_2.836691"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            194..>1445
                     /locus_tag="PKH07_17045"
     CDS             194..>1445
                     /locus_tag="PKH07_17045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36697.1"
                     /translation="MATCTPTRPPIHTPTMSVIPVCSLLDLSCDDCVDYDWVVSCTES
                     YCDKAIHRWTLVSDGMCTEPEGYPRWECSGGAFVGQMGENRNVGTSVKWKSPAEPGAY
                     TIRLIANDVPGGMYSLVPNGVIAGRRDDPETEVDSVVLTVVSAELTSIRFTSDHQSMN
                     DNNRDWDSSGNPYSQPEWVRETEVNNPISHTKGCSIEATVVVKVCPAGLTFHLAGVGP
                     EWLSFSSQGNVSTGQDQPLTLTAAETLPDLVATLEQPITWTTTVSGTEIHLWDSGPHK
                     IYVTYGTPSGSTVTERRIEKTSEWADTATGEEEVADGIHDNLGNADPPYEPSEKPSEG
                     SGWTLLEGNAYGECDEQADLMRYAVLMQGVTPAYLRFVRASTNAGAGNCLDYETRVCS
                     LHGQEWLLLDFGGAGYHGDMNQFEG"
BASE COUNT          349 a          358 c          413 g          325 t
ORIGIN      
        1 aactgattcc cctaccaaaa cgccaacccc gacgatcact tgtggttggg aagtcacatg
       61 gacgcctcga acaccaccca ctatcgttcc acctaccgaa acaccaccca actgcccggc
      121 ctttgaggca ccgcacagcg tttggacacc ggcgcctgga atcaatgtac gattcacgcc
      181 gaccgggacc cagttggcca cgtgtacgcc aacacgtcct ccgattcaca cgccaaccat
      241 gtcggtgatt ccggtttgct ctctgcttga cctgagttgt gatgactgcg tagactatga
      301 ctgggtggtg tcctgtaccg agagttactg tgacaaagcg attcatcgct ggactttggt
      361 atcagacggg atgtgcaccg aaccggaggg ctatccacgt tgggagtgtt cgggcggcgc
      421 gtttgtgggt cagatgggag agaatcgaaa tgtgggtact tccgtgaaat ggaaatcacc
      481 cgcagagccg ggtgcctata cgattcgtct cattgcgaat gacgtgcccg gtggtatgta
      541 cagtcttgtt cccaacggcg tcattgccgg caggcgcgat gatcccgaaa cggaagtgga
      601 cagcgtggtg ttgacagtgg tctcagccga gctcacgagt atccgtttca cttcggatca
      661 ccagtcaatg aatgacaata atcgggattg ggatagtagt ggaaacccat acagtcaacc
      721 cgagtgggtt cgtgagacgg aagtgaacaa tcctattagt cacacgaaag gttgctcaat
      781 agaagctacc gttgtcgtca aggtatgtcc tgcaggcttg actttccatc tggctggggt
      841 tggtccggaa tggcttagtt tctcgtccca gggaaacgtc tccacagggc aagatcaacc
      901 tctcactttg acggctgccg aaacattgcc cgatctagtc gccactctag agcagccaat
      961 tacgtggacg accactgttt cgggaacaga aatccacctt tgggatagcg gtccgcacaa
     1021 gatctatgtg acgtacggta ctccgtcggg cagtactgtg acagaacgtc ggattgaaaa
     1081 gaccagtgag tgggcagata cagcaaccgg agaggaagaa gtcgccgatg gaattcatga
     1141 taatcttgga aacgctgatc cgccttatga gccgagcgag aagccttcgg aaggctcggg
     1201 gtggacactc ttggaaggaa atgcatacgg agagtgtgat gaacaggctg atctcatgag
     1261 atatgccgta ctgatgcaag gtgtcacacc tgcgtatttg aggtttgtga gggctagtac
     1321 caatgcggga gcaggcaact gtctggacta cgaaacaaga gtctgttctc ttcacggtca
     1381 ggagtggctc ctattggatt ttgggggagc aggctatcat ggtgacatga accaatttga
     1441 gggat
//
LOCUS       DAOQUC010001218         1445 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_123986_length_1445_cov_2.756835, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001218 DAOQUC010000000
VERSION     DAOQUC010001218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1445
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123986_length_1445_cov_2.756835"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..380
                     /locus_tag="PKH07_17050"
     CDS             <1..380
                     /locus_tag="PKH07_17050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36698.1"
                     /translation="VGLGLEFLRHIERTVFLIFVLDLSMDKDGWPPAKAYAALRQEIG
                     LYRQEILRRPHLIVFNKVDLMQDEAEVQRLVSETLSTLDTPVEGSFVISAETGAGVQP
                     LRESLVEKAPVWLDCCQEEVQQT"
     gene            377..>1445
                     /gene="proB"
                     /locus_tag="PKH07_17055"
     CDS             377..>1445
                     /gene="proB"
                     /locus_tag="PKH07_17055"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HNT36699.1"
                     /translation="MSRSQASPKDRVEQLLKTTERIVVKVGTNLIDGQKGFNLPMLAG
                     LASDLADLRSQGKQVILVTSGAVGAGRRLIGTIQGYPKSMPERQACAALGQAQLMHLY
                     QELFQHYGCRVAQVLLTEDAVTDRERYVNARNTLEKLLEWGVIPIINENDTVATDELK
                     FGDNDRLSALVASKVHAQLLILLTDVDGLYDQPPQTGKGTLIEYVEHLSPEIESAAGH
                     KGSNYSIGGMKAKIVAVEISMRSGILAAIANGNRFGIVHTILQGEGPATWFLPSGKPL
                     SSKKAWLATSKRVCEGKILVDQGAQDALLGKGKSLLPIGVIQIKGRFKSGDTVSICDS
                     KGKELARGLVNFSQDELILIAG"
BASE COUNT          352 a          353 c          404 g          336 t
ORIGIN      
        1 gggttgggct tggtttggag tttcttcggc acattgagcg gacagttttc ctcattttcg
       61 ttctggatct ctccatggac aaagatgggt ggcctccggc aaaggcctat gccgcattac
      121 gtcaagagat tgggctttac agacaggaaa ttctacggcg gccgcatctg attgttttca
      181 acaaggttga ccttatgcaa gatgaggcgg aagtgcagag actggtcagt gaaactttgt
      241 caaccttgga tactccggta gagggaagtt ttgtcatctc cgcagagacg ggagctggcg
      301 tgcagccact tcgcgaatct ctcgttgaaa aagctccggt ttggttggat tgctgtcagg
      361 aagaggttca gcagacatga gtcgctcaca ggcttctcca aaggatcggg tcgagcagtt
      421 gttgaaaacg acagaacgaa tcgtcgtcaa agtcggcacc aatctcattg acggccagaa
      481 gggattcaac cttcccatgc tcgctggctt ggcatcggat ctcgccgatc tccgatctca
      541 gggcaagcag gtcattctgg tcacatccgg cgcggtcggt gccggtcgcc gcttgatagg
      601 aaccatccaa ggctatccca agtccatgcc tgagcgacag gcgtgcgcag cactcggaca
      661 ggcacagttg atgcatctgt accaggaact cttccaacac tatggctgtc gtgttgcaca
      721 ggtgctcctc accgaagatg ctgtcaccga ccgagagcgc tatgtaaacg cgcggaatac
      781 actggagaag cttctcgagt ggggagttat cccaattatc aacgagaatg acactgtggc
      841 aacggacgag ctaaagtttg gcgataatga ccgactttca gccttggtgg caagtaaagt
      901 tcatgcacag ctgctgattc ttcttacgga tgtggatggt ctatacgatc agcctcctca
      961 gaccggaaag ggaacgctca ttgagtacgt ggagcatctg tcgcccgaga tcgagtctgc
     1021 cgcagggcac aaaggctcca attactccat tggcggcatg aaagcgaaga tagttgctgt
     1081 cgagatttcc atgagaagcg gaatcctggc agccattgcc aacggcaatc gctttggaat
     1141 tgttcacacc atattacagg gagagggtcc tgcaacatgg tttctgcctt caggaaagcc
     1201 gttgtcaagc aaaaaggctt ggctggcaac gagcaagcgt gtctgtgaag gtaaaatact
     1261 tgttgaccaa ggagcccagg atgctcttct gggtaaagga aagagtctct tgcccatcgg
     1321 ggtcatccaa atcaaaggcc gattcaaatc tggagacacg gtctcaatct gtgactccaa
     1381 aggcaaggaa ctggcacgtg ggttggtcaa tttctctcag gacgaactca tcctgattgc
     1441 cggca
//
LOCUS       DAOQUC010001219         1445 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_124024_length_1445_cov_2.331655, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001219 DAOQUC010000000
VERSION     DAOQUC010001219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1445
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124024_length_1445_cov_2.331655"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1445)
                     /locus_tag="PKH07_17060"
     CDS             complement(<1..>1445)
                     /locus_tag="PKH07_17060"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNT36700.1"
                     /translation="GSRLRYCASGGAKLDPAITERLLDLGILMLEGYGLTETAPVISF
                     TPISKPKPGSVGLPLEGIEVRIDKPDTSGQGEICMRGPNLMKGYYQKEAATAEVIRDG
                     WFHTGDLGFLDKDGMITITGRAKEIIILPSGKNIYPEEVEARYESPLIKEICVAPIND
                     AEGVCQGLRAIVVPNVEELAARKAANIYERIRSALTIIGASLPSYMRVTELVLFDEPL
                     PRTRLGKLRRSNIDQMVRQKETAPTKTESVEISAEVQAVLNDPITKRFVKRLSELTDK
                     KEVILPSHDLELDLGLDSLTQVQLTMIVEEEFGVAIPDEDFANIRTVGDILHRIRHGA
                     NEPVTAETTVSWSERLQQQPEVSLESLFTVNRGWIKRILIALLRILVRLLMRFYFRAR
                     IEGLEYIPKDGALLICPNHQSYIDPVLVFSMLPERIMRRAFFVGFEDIFSRPPLSWAT
                     KIGRIILTGGASTWAESLKLSADVLRRKMVI"
BASE COUNT          330 a          397 c          371 g          347 t
ORIGIN      
        1 gatcaccatc ttgcgtctca gtacatcggc tgagagtttc agcgattctg cccatgtgga
       61 tgcccctcct gtcaggatga ttctgccgat ctttgtcgcc cacgacagag gtggacgcga
      121 gaaaatgtcc tcgaatccca caaagaatgc tcggcgcatg atgcgttccg gcaacattga
      181 gaaaaccagc actggatcta tgtagctctg atgatttgga cagatcagca acgcaccgtc
      241 tttcggaatg tattccagcc cttcaattcg cgccctgaag tagaaacgca tcaagagtcg
      301 caccaggatg cgcagcagag cgatcaggat acgttttatc catccccgat tcacggtgaa
      361 cagtgactcc aatgacacct ctggctgttg ctgaagccgt tcactccagg aaacggttgt
      421 ctccgctgta acaggttcgt tggcaccatg acgaattcgg tggagaatat ccccaacggt
      481 tcgaatgttc gcgaaatcct cgtctggaat cgcgacgcca aactcctcct caacaatcat
      541 ggtcagttgc acctgagtca aagaatccag accaaggtcg agctcgagat cgtgcgatgg
      601 caagataact tccttcttgt ctgtcagttc agagagtcgc ttgacaaaac gtttcgtgat
      661 ggggtcgttc aggactgcct gaacttcggc agaaatctcg acggactctg tcttggtggg
      721 agcagtctcc ttctgtcgaa ccatctgatc gatgttggag cgccggagtt ttccaagtcg
      781 tgtgcgcggc agcggttcgt caaaaagaac cagttcagtg accctcatgt aactgggcag
      841 agacgctccg ataatcgtta gcgcggaacg gattcgctcg tagatatttg ccgccttgcg
      901 ggcggccagt tcctcgacat tcggaacgac aatggcacga agaccctggc agacgccttc
      961 tgcatcattg atcggtgcga cgcaaatctc cttgatcaat ggactctcgt atcgcgcctc
     1021 gacttcttcc ggatagatgt tcttgcccga ggggagaatg ataatctctt ttgctctgcc
     1081 agtgatggtt atcatgccat ccttatccag aaaacccagg tccccagtgt gaaaccagcc
     1141 gtcacggatc acctcggcag tagccgcctc tttctgatag tagcccttca tgagattggg
     1201 acctctcatg catatttccc cctggcccga tgtgtccggt ttgtcaattc ggacttcgat
     1261 tccctcaaga ggaagcccga cgcttcccgg cttgggcttc gagattgggg tgaaggagat
     1321 cacaggagcg gtctccgtga ggccgtatcc ctcaagcatg aggattccca gatcgagaag
     1381 cctttcggtt attgccggat caagttttgc accaccagac gcacagtaac gaagcctgga
     1441 gccaa
//
LOCUS       DAOQUC010001220         1444 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_124092_length_1444_cov_3.336933, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001220 DAOQUC010000000
VERSION     DAOQUC010001220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1444
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124092_length_1444_cov_3.336933"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            447..1199
                     /locus_tag="PKH07_17065"
     CDS             447..1199
                     /locus_tag="PKH07_17065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36701.1"
                     /translation="MKVFGVSRRFLVILGLCAVLYIASGVLYYCMKAYIHPDVRLLLK
                     VRGLSVGETPMEEIATLLPESRQRRVEVSFIDGTALWLVSSRRITSQLAERIAQARGS
                     SSSIVEYMDLSYDLALISFDPKRVLNGYAFLGDTAGVFSEIEAESCPTNIEYSLKDIE
                     MLLPKSDYHRTEVTFPDGTLLWIVCSPKQANRILHRVENLRGKATSPLDYTSLSNDAV
                     LIAFDGEGSFIGGLQNEFTYGIFEQKVDERRP"
BASE COUNT          346 a          360 c          407 g          331 t
ORIGIN      
        1 caggcattcg tgccgaagat cttcaggggg gcgggattca tcccgcgcaa gaacgggatc
       61 gtgttaatga gtcgcgctat aaatgccgcc cgacaagact gcagatttga cagaccttcg
      121 cttcgaatcg tgggggtggt ttggtctgca ggggtagcgc ttgccctacc ctccggaaag
      181 ggcgcagcaa gcagcgcccc tacacgagca acgccgggat cagcacccgc acgttacgag
      241 tcgaagggtg cgggcgagcc gctccagctc ctgaagaatt tgaccgtgtt cctcaagtgt
      301 gagagacttg ccatcatgat gagttcatgg actcaacata aatcagcagc cctcagaacc
      361 gcttcgcgaa cctgagggct ggcacccaga gaagtcagtc ggagtatgag aagagagagg
      421 gcacccggca tggagaaact agacacatga aggtatttgg tgtcagccgc agattccttg
      481 taattctcgg cctctgtgct gttctgtaca ttgcttcagg tgtgctttac tactgtatga
      541 aagcatacat tcatcctgat gtgcgtctac tgctgaaggt gagaggtctc tccgtaggag
      601 agacgccaat ggaggagatc gcaacgctct tgccggagtc gcggcaaaga cgggttgaag
      661 tgtctttcat tgacggaact gcgctttggt tggtctcctc gcgacgcata accagtcaac
      721 ttgctgaaag gattgcacag gctaggggat ccagctccag cattgtagag tatatggatc
      781 tttcctatga cctggcactg atttcctttg atcccaagcg cgttttgaac ggatatgcgt
      841 ttcttggtga tacggcggga gtgttctcag aaatcgaagc cgagtcttgt cccaccaata
      901 tcgaatacag cctcaaggac attgagatgc tcttgccaaa gagtgactat catcggacag
      961 aggtgacgtt cccggacgga acgctgctgt ggattgtctg ctcgcccaaa caggctaatc
     1021 gcatcttaca cagagttgag aacttgagag ggaaagccac ttctccctta gactatactt
     1081 ccttgtccaa cgatgctgtc cttattgcct ttgacggtga agggagcttc ataggcggct
     1141 tgcagaacga atttacctac ggcatctttg agcagaaagt ggatgagcgt agaccgtagc
     1201 ctcattgtac ggcttcttgg attccttctc cccctgggag aaggacggga tgagggtaac
     1261 ggagcggggg catcttgctc ccgaatgaaa gaaatgtggg gcgagccgcc ccacctcctg
     1321 aggaatttga ccatgcgcct caaatatgag agacttggca acacgatgag ttcatgaatt
     1381 caaggtaaat cagcagccct cagaaccgct tcgcgaacct gaagtctggc acccagagga
     1441 gtca
//
LOCUS       DAOQUC010001221         1444 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_124129_length_1444_cov_2.591793, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001221 DAOQUC010000000
VERSION     DAOQUC010001221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1444
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124129_length_1444_cov_2.591793"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="PKH07_17070"
     CDS             <1..204
                     /locus_tag="PKH07_17070"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36702.1"
                     /translation="HHVVLYPPMDIGDPSEGEQQRVERVLNELNRLLESEIRSHPTEW
                     AWMHRRWRQKPSPETNGVTDVSL"
     gene            435..1073
                     /gene="lptC"
                     /locus_tag="PKH07_17075"
     CDS             435..1073
                     /gene="lptC"
                     /locus_tag="PKH07_17075"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HNT36703.1"
                     /translation="MSRDSLPTIVLVACVLFMVPWVTSKAREEPPPGMERWKERALEV
                     ESATAEAIPGIQLYIRNFVSTYFPVEATGSYWIISASEARFETMDNLELVRPMLRIVD
                     PEKDDAVTVIRSSSGKCSTNGEYVYLSGNVRTRTYTGDYLETEDLCYDPTQEMAIFSK
                     GPFVLTQASQREAIRGKGIYSDPRLSKMKFGSVQGRVSGGMKGLFQKKEEKK"
     gene            1090..>1444
                     /locus_tag="PKH07_17080"
     CDS             1090..>1444
                     /locus_tag="PKH07_17080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36704.1"
                     /translation="MVRRKGWVRVFELVGLACLLIVAAQAGAQVNLAEVDPLEYVNFV
                     RGDLTIENMNQPNEEILYSNPDDKGTVELEVKTQNSNVKVVAKNIRLTSNKTLVHATG
                     NVNIDDGTTSVRSDSA"
BASE COUNT          389 a          334 c          396 g          325 t
ORIGIN      
        1 catcatgttg tgctctatcc acccatggac atcggagatc ccagcgaagg tgagcagcag
       61 cgcgtcgaac gagtgctcaa tgaactgaac cggcttttgg aatcggagat ccgcagccat
      121 cccaccgaat gggcttggat gcatcgaaga tggcgacaga aaccatcgcc cgaaaccaat
      181 ggagtgacag acgtctcgct ctgaacaaag gccggttttt tcacgtcaga gagacagaga
      241 tgaagcctca ttgatgttgt gtgagcatct tttgaaggtt agttgaacga aatccttgaa
      301 atcacaggca caaagcgctt agaatcagcg caatggtcag gagagtccaa cggctctcca
      361 gcaagttcga gactcgttaa gaggtctctt tcctgattag accttgtttg tggaaagtct
      421 gggagaagga caggatgtca agagacagcc ttccgacaat agttctggtg gcctgtgtgc
      481 tcttcatggt accttgggtc accagcaaag cccgagaaga gccccctcca ggtatggaac
      541 ggtggaagga acgtgccttg gaagtggaat cggcaaccgc agaggccatc cctggaatac
      601 aactctatat ccggaacttt gtctcaactt atttccctgt tgaagcaacc ggatcttatt
      661 ggataatctc cgccagcgaa gcgcgctttg aaaccatgga caaccttgaa ctggtccgac
      721 ccatgcttcg catcgttgat ccggagaagg acgatgcggt gacggtcatc cgcagcagtt
      781 ccgggaagtg cagcaccaat ggagagtatg tctacctgag tggaaacgtc cgaacgagaa
      841 cttacacagg ggactatctt gagacagagg atttatgcta cgatcctacc caggagatgg
      901 cgattttctc caaaggcccg tttgtactca cacaggcctc gcagcgggaa gccatccgtg
      961 gcaaagggat ttacagtgat ccgcgtcttt cgaaaatgaa attcgggagc gttcaaggaa
     1021 gagtctctgg cggcatgaaa ggtctctttc aaaagaagga agagaagaaa tagttttgga
     1081 ggattacaga tggttcgtcg caaaggttgg gtcagggtgt ttgaacttgt cggtttggcg
     1141 tgtctgttga ttgtcgcagc tcaggcaggt gcccaagtca acttggccga ggtggatcct
     1201 cttgagtacg tcaacttcgt caggggagat ctgaccatcg agaacatgaa tcaaccaaat
     1261 gaggaaattc tttattcgaa tcctgacgac aagggaactg ttgagttgga agtcaaaacg
     1321 cagaacagta atgtcaaagt ggttgccaag aacatccgcc ttacttccaa caagactttg
     1381 gtgcatgcaa ctgggaacgt caacattgac gacggaacga ccagtgtcag gagtgactct
     1441 gcca
//
LOCUS       DAOQUC010001222         1443 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_124266_length_1443_cov_2.677233, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001222 DAOQUC010000000
VERSION     DAOQUC010001222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1443)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1443)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1443
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124266_length_1443_cov_2.677233"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1424)
                     /locus_tag="PKH07_17085"
     CDS             complement(<1..1424)
                     /locus_tag="PKH07_17085"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNT36705.1"
                     /translation="MADFSSPGSVQAFVNGACVSGAPSATYKSGGKDIPLYQVSDEVA
                     AVVPNPGNDVLPVRNDGLSLMKKSNRRQQTGTTFPVFVNGVTGKKQVLTDTLIVCLQS
                     PDVVAQLETYCAQNGAQAIRQLRGTEDQFLVKVAGDTLNAAVQFGAQAFVAWAEPDFV
                     SEYKPEYLPNDTYFGLQWPVWNTGQSGGVEDEDIDGAEAWDIAQGSGTVVAVLDDGVD
                     IDHADLAANVWTNGGEIPGNSTDDDSNGYVDDVQGWDFYDNDNNPRPIDPADNHGTAV
                     AGLIAAIGDNSAGIAGVSYQAKVLPLKIFRFNYYASASAIAEAIRYAADNAHVVNNSW
                     TGGGVSAALESAVDYANGNGLRGPLGSPVFFSSGDAGSGWRQYTQTGIPAGTHVFAWV
                     YFKDESGSAGEDSVWLDDITFPGYDMIDFEGGPLPGSWSNSGDEVWALAGDGVHYRSA
                     TQSIKAGAIGNSEASIISCELTVPG"
BASE COUNT          325 a          425 c          355 g          338 t
ORIGIN      
        1 ccaggcacag tgagctcaca cgatatgatg cttgcttcac tgttgcctat cgcgcccgct
       61 ttaattgatt gtgtggcgga tcggtaatgc acaccatcac ctgcgagcgc ccaaacctca
      121 tctccggagt tactccagct tcctggcaag ggaccgcctt cgaagtcaat catgtcatag
      181 cccgggaagg taatgtcatc cagccagacg gagtcttcgc cagccgaacc actctcgtcc
      241 ttgaagtaga cccatgcgaa gacgtgggtt ccagcaggaa tgcctgtctg cgtgtactgt
      301 ctccagcctg aacccgcatc gcctgatgag aagaacactg gagaaccaag aggtccccgc
      361 agaccatttc cgttggcgta gtcaacagcc gactcaagag cggcactgac gcctccacct
      421 gtccaactgt tgttgactac atgggcgttg tccgcagcgt agcggatcgc ttctgcgatg
      481 gctgaagcac tcgcatagta gttgaaacgg aagatcttga gtgggagcac tttcgcctgg
      541 taactcacac cggcgatacc agctgaattg tctccaatgg cagcgataag gcctgccacc
      601 gctgttccgt gattgtccgc tgggtcaatc ggacgtggat tgttgtcatt gtcatagaaa
      661 tcccatcctt gaacatcatc aacgtagcca ttgctatcgt catcagtgct gttgcccggg
      721 atttcaccac cgtttgtcca gacgtttgct gccagatcgg catggtcaat atcaacgcca
      781 tcatccagta ccgcaaccac cgtacccgaa ccttgggcaa tatcccaagc ttccgcaccg
      841 tcaatatctt catcttctac accgccggac tgaccagtgt tccaaacagg ccattgaagt
      901 ccgaaatagg tgtcgttggg aagatactct ggcttgtact cactgacaaa atcaggctca
      961 gcccaagcga cgaaagcctg cgcaccaaac tgaactgctg cgttgagggt gtcgccggca
     1021 accttgacca agaattgatc ctctgtacca cgcagttgtc gaattgcctg ggcaccattc
     1081 tgtgcgcagt aggtttccaa ctgagcaacg acgtctggtg actgcaggca gacaataagt
     1141 gtgtccgtca atacttgctt cttgccggtc acgccattca cgaaaacggg gaatgttgtc
     1201 cctgtctgct ggcgtctgtt ggacttcttc atcagactta gtccatcgtt tcgcaccggc
     1261 agaacatcgt tgcccggatt gggaacaact gctgccactt cgtcagacac ctggtacagc
     1321 ggaatgtcct ttccgccact cttgtacgtg gcgcttggtg cacccgacac acaggcaccg
     1381 ttgacaaatg cttgaaccga tcctggactg ctgaagtccg ccacgaactc cttcgcaata
     1441 ttg
//
LOCUS       DAOQUC010001223         1443 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_124274_length_1443_cov_2.604467, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001223 DAOQUC010000000
VERSION     DAOQUC010001223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1443)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1443)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1443
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124274_length_1443_cov_2.604467"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            211..684
                     /locus_tag="PKH07_17090"
     CDS             211..684
                     /locus_tag="PKH07_17090"
                     /inference="COORDINATES: protein motif:HMM:NF016160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HNT36706.1"
                     /translation="MCYCNPIMKENPALEEIGVVREISGDQVTVVLQRTDRCHSCTLC
                     KSGPEGMFLTVHDPIGVSVGDRVVIGRSSEALVRATLIVFVVPLITFFGGAFVAHQLS
                     QGGSELVLACGAGVGLIVGALGVWFYERTMKEGARQQFQPYIERVCERGSSTQDR"
     gene            743..>1443
                     /locus_tag="PKH07_17095"
     CDS             743..>1443
                     /locus_tag="PKH07_17095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015947027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNT36707.1"
                     /translation="MGTSRKRKHGLPLVTTSTDAILESISDGVFTVDQQWRVTSFNRA
                     AEDITGIPRDEAVGRLCSEVFRSSMCERECALRKTLDTGKPIIGKTGYIIDAEGARIP
                     ISVSTAVLRDDQGKVVGGAETFRDMSEVETLREELEGRHRIGDLFSRSPLMQRMLDVL
                     PAISASPSTVLILGETGTGKELLARTIHSLSSRKDAPFIAVNCGALPDTLLESEMFGY
                     KAGAFTGADRDKPGR"
BASE COUNT          335 a          339 c          416 g          353 t
ORIGIN      
        1 ctacctggcg gtagatttcg aatggatcaa tccctaatcc tgggctcgcc ggtggcgagg
       61 agtaataaaa tcaccaagtt ataccaacga tcacctctct tctttcgaac attcctgacg
      121 agaatgtaac aaattcggaa cccaatgtca agagaacatt cagaaattgt tggcaatgca
      181 agggatacca tatatcccaa ggctttgata atgtgctatt gtaatcccat catgaaggag
      241 aatcctgctc ttgaggaaat aggagtcgtt cgagagatct cgggagacca ggtcaccgtc
      301 gttctccaaa gaacggaccg ctgtcattct tgtaccctgt gcaagtccgg cccagagggg
      361 atgttcttga cggtgcatga tcccataggc gtttcggttg gagatcgcgt cgtcatcgga
      421 cgctcatcgg aagctcttgt aagagcgact cttattgtct ttgtcgtgcc gctgattacc
      481 ttttttgggg gagccttcgt cgcgcatcag ttgtcacagg gtggtagtga actggtgttg
      541 gcttgcggtg cgggagttgg tctcatcgtg ggggcactcg gcgtctggtt ctatgagcga
      601 accatgaagg aaggcgcgcg acagcaattt cagccctaca tcgaaagagt gtgcgaacgt
      661 gggagcagca cgcaagatag gtgatgactc cagacttgcc gttcgtcttt cgcatcaggc
      721 atgctatggt tcatctatga atatgggtac atcgcgaaaa cgaaaacacg gtctccctct
      781 tgtgaccacc tcgacggacg ccattctgga gagtatttcc gacggagtct tcacggtaga
      841 ccagcaatgg cgtgtgacct cattcaaccg agccgcagaa gacatcaccg gaatccccag
      901 agatgaggcc gtaggccgtc tgtgctctga ggtttttcgc agcagcatgt gcgaaagaga
      961 gtgcgctttg cgcaagacgc tcgatacggg caagcccatc atcggcaaga caggatacat
     1021 cattgatgcg gaaggagctc gcattccaat cagtgtctct acggcggttc tgcgagatga
     1081 tcagggtaag gtcgttggtg gtgcagaaac gtttcgagat atgtccgagg tcgagacttt
     1141 gcgtgaggag ttggagggac gacatcggat tggtgatctg ttcagccgaa gtccgctgat
     1201 gcaacggatg ttggatgtgc ttccggcgat ttcggccagt ccaagcacag tcttgattct
     1261 gggggagacg ggtactggaa aagaactctt ggcgagaacc attcattcac tgagttcgag
     1321 aaaagatgct cccttcattg ctgtcaactg cggcgcgctt ccggacacgt tgttggagtc
     1381 tgagatgttt ggctataagg ctggcgcttt caccggagct gatcgggaca aacctggacg
     1441 ttt
//
LOCUS       DAOQUC010001224         1442 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_124384_length_1442_cov_2.804614, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001224 DAOQUC010000000
VERSION     DAOQUC010001224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1442
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124384_length_1442_cov_2.804614"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /locus_tag="PKH07_17100"
     CDS             <1..246
                     /locus_tag="PKH07_17100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006001313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HNT36708.1"
                     /translation="DMEQVKLQFTDGAVNAVVDEALKRKTGARGLKAIMERVMLDIMY
                     ELPGSRTVKELVVSEDMVLAATEIWNTGEGSPKAQTA"
     gene            312..860
                     /locus_tag="PKH07_17105"
     CDS             312..860
                     /locus_tag="PKH07_17105"
                     /inference="COORDINATES: protein motif:HMM:NF014602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HNT36709.1"
                     /translation="MAFQIGDVVVKPSLGICKIRAKTTLTVEGKQQEFFVIYSGDVRV
                     LVPVEQAKKGTIRHVMSEKVQKKVLKDLQEPLRLPTDQHGDILEEAYRLDADQSNAII
                     KERDPEKLCGLIRLLFNKMKDHQQLEDKAEQSLFSEALSLLSEELAYLEKTIKGRMTS
                     QIKQMLAEARKKRKKEKAGSVD"
     gene            919..>1442
                     /locus_tag="PKH07_17110"
     CDS             919..>1442
                     /locus_tag="PKH07_17110"
                     /inference="COORDINATES: protein motif:HMM:NF012981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HNT36710.1"
                     /translation="MNWQDKSVLLIWNRKAGSSRVLSVQSKWIETLTQLCGRLDVRIP
                     NNPEQIESILQDVMPCARPDVVIAGGGDGTLHLVVRGLLKADSMRRASVAQLPFGSGN
                     DFLRQYGFHRLNSRNLFRFVQCSLESLDVGWCAEVPFVNGVGFGLAADTGRRFAEMAN
                     RSALNYLFASIHSIV"
BASE COUNT          365 a          338 c          401 g          338 t
ORIGIN      
        1 gacatggagc aggtaaagct ccagtttacc gatggagctg tcaatgctgt tgtggatgaa
       61 gcgctcaaga ggaagactgg tgccagaggc ttgaaagcca ttatggagcg agtgatgctg
      121 gatatcatgt atgagctccc tggttccagg acagtgaaag aacttgtggt cagcgaggat
      181 atggttctgg ccgccacaga gatctggaat acgggtgagg ggtctcccaa agcccaaaca
      241 gcatagaaat agcaagagat gagactggtc ttttcctatt ggggagaagg cagacacggg
      301 agttagttca catggcattt cagattggcg atgttgttgt caagccatct ttgggaatat
      361 gcaaaatccg ggcaaagaca accctcacag ttgagggcaa acagcaggag ttcttcgtca
      421 tctactcggg ggatgtccgt gttctcgtgc ctgttgagca agccaagaaa ggtaccattc
      481 gccacgtaat gtccgagaag gttcagaaga aggttctcaa ggatcttcaa gaacctctac
      541 gccttcctac cgatcagcac ggggacatcc ttgaagaggc ctatcggctt gacgccgatc
      601 agtcaaacgc catcatcaag gaacgagatc cggagaaact ctgcggactg atccgactcc
      661 ttttcaacaa gatgaaggat catcagcagt tggaggataa agcggagcag agtttgttct
      721 ccgaagcgct ttcgcttttg tcggaagagt tggcgtatct tgagaagact atcaaggggc
      781 gcatgacctc tcagatcaag caaatgcttg ctgaggccag aaagaagcga aagaaagaaa
      841 aggccggaag cgttgactag ccttttcgaa tctcactgtc ggtctctcct ctgattcctg
      901 cgccgtgggc tgcatgctat gaactggcag gacaagagcg tcttactcat ctggaatcga
      961 aaagctggtt cgagcagggt gctctccgta caatccaagt ggatagagac gttgacacaa
     1021 ctctgtggcc gtctcgatgt tcgaattcca aacaatcccg aacaaattga gtccatactc
     1081 caagacgtca tgccttgcgc caggccagac gtcgtaatcg ccggtggcgg cgatggaaca
     1141 cttcatcttg tggtgcgagg gctgctgaag gctgattcca tgagacgagc aagcgtcgcc
     1201 caattgcctt ttggcagtgg gaacgacttt cttcgtcaat atggttttca caggctcaac
     1261 tccagaaacc tcttcaggtt tgtccagtgc tcccttgaat cacttgatgt gggctggtgt
     1321 gcagaggttc cttttgtcaa cggcgttgga tttggacttg cggcggacac aggaaggcga
     1381 ttcgccgaga tggcgaatcg ctcagcgttg aactaccttt tcgccagcat tcactccata
     1441 gt
//
LOCUS       DAOQUC010001225         1442 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_124387_length_1442_cov_2.771449, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001225 DAOQUC010000000
VERSION     DAOQUC010001225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1442
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124387_length_1442_cov_2.771449"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="PKH07_17115"
     CDS             <1..232
                     /locus_tag="PKH07_17115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36711.1"
                     /translation="AGTKSVYRFAGYRRRYATLTRADSLSASIPNAKNACFQMHTRFC
                     RQMVSEPTTEASGEGLQLLRVSSTDGCRRDVG"
     gene            528..>1442
                     /locus_tag="PKH07_17120"
     CDS             528..>1442
                     /locus_tag="PKH07_17120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010873629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-directed DNA polymerase"
                     /protein_id="HNT36712.1"
                     /translation="MTLNATSLNWAIEFVAKHSDSDLFPRVLEIEAIHALRNTFVELV
                     EGKTLSDLEIGASRRFIVPKDEVSYRQATQLHPQDSIILSAVVHQFGRGIESRRLPAD
                     RVFSYRFSPTVDHGLYGSQTGWNKFWNAAGQRSFQCAAILYCDIADFYNQIYHHTVEN
                     QLIESEFPNQAAKWVIKLLESTTAGVSRGIPIGPHGTHLIAEATLIPVDNSMSSNGIA
                     FLRYADDIVVFCDSEKATRQALGTIASILDKQQRLTLQRHKTRFFKPNEFRALCSEMI
                     EDRPISQDEDDILQLIRKYSEDDPYKLVS"
BASE COUNT          352 a          399 c          362 g          329 t
ORIGIN      
        1 ggccggaacc aagagcgttt accgctttgc aggataccgg cggagatatg cgacactcac
       61 ccgagccgac tctctctccg ctagtatccc aaatgcgaaa aacgcttgct ttcagatgca
      121 cacaaggttc tgccgacaaa tggtttctga accgaccaca gaagcatctg gagaaggtct
      181 tcagttgttg cgagtcagca gcacagatgg ctgccgccgc gacgtgggtt gagtacagga
      241 gacgccggtc tcctggagaa caccgacgac gaaggtgcac tacctgccac aggtagcacc
      301 accacaagtg agtatggcgt ccccggaatt cgtcctttat tctggtcaca tgtcgccgcc
      361 gaatctgtga tctggcttga cttcccgccc acgtccggtc acagtctgtg caggattagc
      421 tcgcccgttg ccgccccgca aagaataaga ggcgatgcgg aaggccgttg tgagatctat
      481 gatatagtgc acctgacaca tgcgattcct tcttgagaga acctgcgatg actctgaatg
      541 ccacctcgtt gaattgggcg atcgaatttg tcgccaagca ttctgacagc gatctctttc
      601 caagggttct ggagattgaa gcgattcacg cccttcggaa tacattcgtt gagttggttg
      661 aaggtaagac attgtccgac ctggaaatcg gtgcgtctcg tcggttcatt gtgcccaaag
      721 atgaggtctc ttataggcag gcaactcagc ttcacccgca ggattccatt attctatcgg
      781 ccgtggttca tcaatttggc cgaggcatag aaagtagacg ccttcctgca gaccgtgtct
      841 tcagctatcg cttctcaccc actgtggacc atggcttata tgggagccag accggatgga
      901 acaagttctg gaacgctgct ggccagcgca gttttcaatg tgccgcgatc ctatactgcg
      961 atatcgctga cttctacaat cagatctacc accatacggt tgagaatcag ttgattgaat
     1021 cggagtttcc aaaccaagca gcgaagtggg taatcaagct ccttgaatcc actaccgcag
     1081 gcgtctcccg cggaatcccc atcggacctc acggtacaca tctaatagct gaagccacac
     1141 tcatccccgt tgacaacagc atgtcctcaa acgggatcgc gttcctacgc tatgctgatg
     1201 acatagtggt cttctgcgac tcagaaaagg ccacacgcca agcactcggg accattgcat
     1261 caattctgga taagcagcag agactgactc tgcaacgtca taagacacga ttcttcaaac
     1321 ccaacgagtt tcgggctcta tgcagcgaaa tgatcgagga ccgcccgata agtcaggatg
     1381 aggatgatat cctgcagttg atccggaagt actccgaaga cgacccgtac aagctcgttt
     1441 cc
//
LOCUS       DAOQUC010001226         1442 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_124468_length_1442_cov_1.687815, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001226 DAOQUC010000000
VERSION     DAOQUC010001226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1442
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124468_length_1442_cov_1.687815"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            58..1368
                     /locus_tag="PKH07_17125"
     CDS             58..1368
                     /locus_tag="PKH07_17125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36713.1"
                     /translation="MQTALTEHPQRLKEREAVDSVCRDLTIVSDSIGDVLQTLKSLPA
                     PIIMDAESPEKAVVDEIFAGSQAIVAELRGQLESLNMAWRSSQIPVKRNVRTWRTRHR
                     EHLGRYAKAEEESQEAREKLKAIEMLQNQAAAMQREIGQLKADLQKYEEVRDGFNEAW
                     ATWTEKHSERGSLLAGACAGLTEKSAGEIKAEIQRGADIDGPLEELKAALTGCNITQD
                     RWEKLREYLEADQGSPAKAWMKIMEQLRPLAELEDSDVSQGSTIPEVGDWDLTENMQR
                     RIIQRLNPQQWLEIALISLRDKPVFFYCPNPNERIPFDSASAGQQATALLKVLLGESS
                     GPLIIDQPEDDLDNCVIWEIAEAIWKAKECRQLIFSSHNANLVVNGDAELVVRFGYHD
                     ESERTKGKIVCEGAIDVREVREAITEVMEGGEKAFRLRYEKYGF"
BASE COUNT          386 a          310 c          473 g          273 t
ORIGIN      
        1 tctgttcaga ctcaggtgga cgccatcaag aagacattgc cgacggtatc tgacgctttg
       61 cagacggcat tgaccgagca ccctcagcgg ctgaaggaaa gggaggcagt tgactcagtc
      121 tgcagggacc ttacgatagt gtcggattcg ataggcgacg tccttcagac gttgaaatcg
      181 cttccagcac cgattattat ggacgcagag tcccctgaga aggctgttgt ggatgaaata
      241 tttgcgggat cgcaggcgat agtggcggaa ctgcgaggac agcttgaatc gctcaatatg
      301 gcgtggcgaa gcagccaaat acctgtcaaa agaaatgtgc gaacgtggcg gaccagacac
      361 cgtgaacatc tgggtagata cgcaaaggct gaggaggaat cacaggaagc gagggagaag
      421 ttgaaggcga ttgaaatgtt gcagaatcaa gcggctgcaa tgcagaggga gattggccaa
      481 ctcaaggctg atttgcagaa gtacgaggaa gtacgggatg gtttcaatga ggcatgggca
      541 acttggacag agaaacacag tgagcgaggc agtttactgg ccggagcatg cgctggattg
      601 accgagaaat cggcaggcga aataaaagcg gagatccaga ggggtgcaga tatagacggg
      661 cccctggagg aactcaaggc ggctctcaca ggatgcaaca tcacacagga tcggtgggag
      721 aagcttagag agtatcttga agcggatcag gggtcgcctg caaaggcctg gatgaagatc
      781 atggagcaac tgcggcccct ggcagaactg gaggacagtg acgtgtcgca aggcagcaca
      841 atccccgaag tcggagactg ggacctaacc gagaatatgc agaggcgcat aattcagcga
      901 ttgaacccgc agcaatggct tgagatagct ctcataagcc tgagagacaa gcccgtgttc
      961 ttctattgtc ctaatcccaa cgagcggatc ccattcgaca gtgcatcggc cgggcagcag
     1021 gcgactgccc tattgaaggt attgttgggg gagagcagtg gaccgttgat tatcgaccaa
     1081 cccgaagatg atctggacaa ctgtgtcata tgggagatcg cagaagcaat ctggaaggcc
     1141 aaggagtgtc ggcagttgat cttctcaagc cacaatgcga accttgttgt caatggcgat
     1201 gcggaacttg tcgtccgatt cggctatcat gatgaaagcg agaggacgaa ggggaagatc
     1261 gtgtgcgaag gtgcaattga cgtgcgagag gtcagggagg ctattacgga ggtcatggaa
     1321 ggcggtgaga aggctttcag gttgcggtat gagaagtacg ggttctgatc agttggaggt
     1381 gaatccctta cgcggggccg gacccctgtc tgccgggtga cagggcgtgg ccggcaaagc
     1441 gg
//
LOCUS       DAOQUC010001227         1440 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_124662_length_1440_cov_3.206498, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001227 DAOQUC010000000
VERSION     DAOQUC010001227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1440)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1440)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1440
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124662_length_1440_cov_3.206498"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            153..>1440
                     /locus_tag="PKH07_17130"
     CDS             153..>1440
                     /locus_tag="PKH07_17130"
                     /inference="COORDINATES: protein motif:HMM:NF015291.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HNT36714.1"
                     /translation="MSRFGRILLKTSGAVPTVRFQFACERPMVVQERLLRHIIRSNED
                     TAFGKEHHFDQIHSIADFQRQLPIGSYEYHEPYIQKMLQGEPRQLTAQPPILFAMTSG
                     TTGRRKYIPVTQESRVAKSKVMRVWLHLALREHPGILNGRILSVVSPEIEGYTSSGIP
                     YGAESGHAYKNLPLLIRQQYCVPHDVFTIKDYDSRYYVLLRMAVEQEVSTIATCNPST
                     IVLLCERMDLWKEDILKDIYAGTIRDDLVLPDDIRSRLQSHIEPNPDRARVLESCISR
                     NGGVLAPNCCWDKLVMVSCWKAGSVKLYLRDFPRFFPHQTAVRDWGYLASELRGSIPI
                     SDSGEGGVLTIDTNFFEFLPEEESESSQKNYLTVDQLSVGQRYYVYVTTLGGLYRYEM
                     NDLVEVTGFFRSSPEIQFIRKSQGFCSVTGEKLYEDQ"
BASE COUNT          349 a          357 c          390 g          344 t
ORIGIN      
        1 aggcttgggt gtacacgttc tcgaaaaacg tgtcaacgag tttgggcagt ttctcggtgc
       61 aatctgagca cgagcggaaa actttgcgtt gctcagagtg ccctagctga aactggctca
      121 aggaatagat ttttcgaagg gattcttaga aattgagcag gtttgggcga atacttctca
      181 agacatcagg tgctgttccc acggtgcgct tccagttcgc ttgtgagcgg ccgatggtcg
      241 tacaggagag actgcttcgg catattatcc gaagcaacga ggacaccgcc tttggcaagg
      301 agcatcattt cgatcagatt cactcgatcg ctgactttca gcgtcagctg ccaatcggtt
      361 cgtacgagta tcacgagcct tacattcaga agatgctgca gggcgaaccc agacaactga
      421 ccgcccaacc gcctatcctg tttgccatga cgagcgggac gacaggacga cgcaagtata
      481 ttccagtgac ccaggagtct cgtgtcgcga agtcaaaagt catgcgtgtc tggctccatc
      541 tggcgcttcg ggagcatccc ggaattctca acggaaggat cctgtccgtg gtcagtcctg
      601 agatagaggg ctatacctct tcgggcattc cctacggagc agaatcaggt cacgcctaca
      661 agaaccttcc gctacttatc aggcaacaat actgcgttcc acacgatgtg tttacaatca
      721 aggactatga ttcccgctac tacgtgctgc tcaggatggc ggtcgagcag gaagtcagca
      781 cgatcgcgac gtgcaacccg agcaccatcg ttctgctgtg tgaacggatg gatctttgga
      841 aggaagatat actcaaagac atctacgcgg gaacaattcg tgatgacctt gtgttgccgg
      901 acgacattcg ctcacgactt cagtctcaca ttgaacccaa tccggatcgc gcgagagttc
      961 ttgaatcctg tatcagcagg aatggtggcg tcctggcacc caattgctgc tgggacaagt
     1021 tggtaatggt cagttgttgg aaggctggca gcgtcaaact gtatctgcgg gatttcccac
     1081 gattcttccc tcatcaaacc gcggttcgag attggggcta tctggcaagc gaactcagag
     1141 gctccatccc gatctccgat tccggagaag gaggggtgct gaccattgat acgaacttct
     1201 ttgagtttct gcctgaggaa gagtcagaga gttctcagaa gaactatctg actgtagatc
     1261 aactctcggt agggcaacgg tactacgtct atgtcacaac tctgggcggc ttatatcggt
     1321 atgagatgaa cgatctggtc gaggtcacag gattcttccg aagttcacca gagattcagt
     1381 tcattcggaa aagtcaggga ttctgctccg tgactgggga gaaactctat gaagatcagg
//
LOCUS       DAOQUC010001228         1439 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_124823_length_1439_cov_2.995665, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001228 DAOQUC010000000
VERSION     DAOQUC010001228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1439
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124823_length_1439_cov_2.995665"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..983
                     /locus_tag="PKH07_17135"
     CDS             <1..983
                     /locus_tag="PKH07_17135"
                     /inference="COORDINATES: protein motif:HMM:NF039288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNT36715.1"
                     /translation="PKTAETLNLIGEKELAMMKKSVRLINCARGGIINEEALAKALQE
                     GKVAGAAVDVYTKEPPEPTNPLLKAPNCLCAPHLGASTTEAQVNVAIEVCEQVVDALT
                     GKGVRNAANIPYVDPDLLETMTPYLRLAEKMGLLASQIKRGAPQKITATFSGPVADLE
                     VKSLTSAIMVGIFQPHLEYSINMVNAPVIAKERGVQITEQKSTDTRDYADLIQLTLCT
                     SDGDVSLDGTVFAKNDARIVRIDGYHVDARPYGQMVLLRNKDLPGVIGSVGAILGKHN
                     INISDMTLGRIDRGGEAVTVLNVDEVVPEEALKDIGKIPSVLKVQQLRLS"
     gene            1081..>1439
                     /locus_tag="PKH07_17140"
     CDS             1081..>1439
                     /locus_tag="PKH07_17140"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HNT36716.1"
                     /translation="MFPTLFEIGSFRLGTYGVMMAIGALVSIKLASRESERAGMNKDQ
                     FYNFAVYCLLGSIAGARLLYIIVEWDEFLKAPLGMLFAREGFVFLGGFLTGVLVGWWY
                     VRKLRLGFLRTADFIAP"
BASE COUNT          324 a          381 c          405 g          329 t
ORIGIN      
        1 cacccaagac ggcggaaacg ctgaatctta tcggcgaaaa ggaactcgcc atgatgaaga
       61 agagcgttcg gctgatcaac tgcgctcgcg gcggtattat caacgaggaa gcgctggcga
      121 aggcattgca ggaaggcaaa gtcgcaggcg ctgccgttga cgtttacacc aaggagccac
      181 ccgagccgac caatcctctg ctcaaggctc ccaattgtct ttgtgcacca catcttggtg
      241 cgtctactac ggaagcccag gtcaacgtcg ccatcgaagt ctgtgaacag gttgttgacg
      301 ccttgacggg caaaggagtc cgcaatgcgg cgaacatccc ctatgtggat cctgatctgc
      361 tggaaaccat gaccccatat ctccggctgg cggagaagat ggggctgttg gcctcgcaga
      421 ttaagagagg cgctcctcag aagattaccg cgactttcag tggacctgtt gcggacttgg
      481 aagtcaaatc actgaccagc gcgataatgg tcggcatctt ccagccacat ctggaatact
      541 ccatcaatat ggtcaatgca ccggtgatcg ccaaggaacg cggcgtccag atcacggaac
      601 agaaatctac ggacacccgt gactatgccg atctcatcca actgacgctc tgcacctcgg
      661 atggggacgt ctctttggat ggcacggtct ttgccaagaa cgatgcccgg atcgtgcgaa
      721 ttgacggcta ccatgtggat gcacgtcctt acggtcagat ggtgcttctg cgcaacaagg
      781 atcttcccgg cgtgatcggg agcgttggcg ctattctcgg aaaacacaac atcaatatct
      841 cggatatgac actgggtcgt atagaccgag gtggcgaagc ggtgaccgtg ctcaacgtag
      901 acgaggtcgt cccggaagag gccttaaagg acatcgggaa aattccctcg gttctcaaag
      961 tgcagcaact gcgtctgagc tgatcccacg ggtgggctat gcccacttcg accggtgacc
     1021 tgatcgcaac ggaagacccg cacttcctcg gtgcgggttc ttcaatgagg attgcttttc
     1081 atgtttccaa cactctttga aatcgggtca tttcgcctgg gtacctatgg cgtgatgatg
     1141 gcgatcggtg ctctggtttc catcaagctg gcatcccgcg aatcggaaag agccgggatg
     1201 aacaaagacc agttctacaa ctttgcagtc tattgtctgc tgggatcgat tgccggtgcc
     1261 agactcctct acatcatcgt agagtgggat gagtttctca aggcacccct gggaatgctt
     1321 tttgcacgcg aagggtttgt ttttctcggc ggatttctga ccggtgtgct cgttggctgg
     1381 tggtacgtac gcaaactgag acttggtttt cttcgaacgg cggatttcat cgctccgta
//
LOCUS       DAOQUC010001229         1439 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_124829_length_1439_cov_2.913295, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001229 DAOQUC010000000
VERSION     DAOQUC010001229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1439
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124829_length_1439_cov_2.913295"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1439
                     /locus_tag="PKH07_17145"
     CDS             <1..>1439
                     /locus_tag="PKH07_17145"
                     /inference="COORDINATES: protein motif:HMM:NF012271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HNT36717.1"
                     /translation="VVAWQQNADAYCDGYELYRSNSLSGWTLLADKTVLAKTTTNYTV
                     TGLTSGMAYYFKLRAISSTGKPSVFSDVYGAVPTGNTPPVLIVEGYDRWDTQSGRGGS
                     FEGAYYHGAAIANHGMSFDTCDNAMVISGNVILSDYDLAVWVLGEESTTEETFSSTEQ
                     GLVSTYLDGGGNLFVSGAEIGWDLDRATGPTTADRAFYNNYLKADYTADDGGTLTAQG
                     FAGSIFASVGSFDFDFYYIAEWPDVMAPYGGSTVCMTYVGGTAGNAAVQYDGETFNVV
                     NMGFGFETINGESNRNAIMAEALDFLLKDTGPRDTPTDTPTGTITPTPTITPTPTDTL
                     SATPADLIVDNVDPGYSDSYTESEWLLGTSAAGRYGASYRYNLITAGGTDTATWQVTI
                     PTTGYYTVSVWYSVGTNRATAAPYTVNYTGGSQLFLVNQQSNGGQWNALATFYFEAGN
                     HTVLLQDNATYPSPGVVIADAIRWSWSSP"
BASE COUNT          327 a          379 c          390 g          343 t
ORIGIN      
        1 tgttgttgcc tggcaacaga acgccgatgc ctactgtgac ggctatgagt tgtaccgaag
       61 caactcactt tctggatgga cgttgcttgc tgacaagacg gttcttgcca aaacgacgac
      121 gaactatacc gtgactggcc tgacgtcagg aatggcttat tacttcaaac ttcgagcaat
      181 ctcgagcacg ggaaaaccca gcgttttctc ggacgtctat ggtgctgtgc caacggggaa
      241 tacgccgccc gtactcattg ttgaaggtta cgaccgctgg gatacccagt ctggaagggg
      301 tggctctttt gagggagcct actatcatgg ggcggccatc gccaaccatg gtatgtcatt
      361 tgacacatgt gataacgcca tggtcatctc cggcaatgtg attctttcag actatgatct
      421 tgcggtgtgg gtgctcggtg aggaatccac tacggaggag actttcagtt cgactgagca
      481 gggcttggtc agtacctatc ttgacggcgg cggaaatctg ttcgtttctg gagcggagat
      541 cggttgggat ctcgacaggg ctacgggtcc gactacggca gatcgggctt tctacaacaa
      601 ctacctgaaa gcggactata ctgccgatga cggggggact cttactgccc aaggctttgc
      661 cggaagtatc ttcgcgagtg ttggttcctt cgacttcgat ttctactaca tcgcggaatg
      721 gcctgatgtg atggctccct acggtggttc gacggtctgt atgacctacg tgggtgggac
      781 tgcgggtaac gcagcagtgc aatacgacgg tgaaaccttc aacgtcgtca acatgggttt
      841 tggctttgag accatcaacg gtgaatcgaa tcgaaatgcc atcatggcag aggcgctcga
      901 tttccttttg aaggataccg gtccaagaga cacgccgacc gatacaccaa cggggacaat
      961 aacacccacg ccaaccatca cacccacacc cacagatact ctgtctgcaa ctccagcgga
     1021 tctcatcgtg gacaacgttg atcccggtta ctccgacagc tataccgaaa gtgaatggtt
     1081 actgggaaca tctgcggctg ggcgctatgg agccagttac cgatacaacc tgatcaccgc
     1141 tggcggtaca gatacggcaa catggcaagt cacgattcct acgacgggct actacactgt
     1201 ctccgtctgg tactctgtcg gcaccaaccg agccacggct gcaccctaca cggtcaacta
     1261 cacgggtggc tcccaactgt ttcttgtgaa tcaacagtcc aacggtggtc aatggaatgc
     1321 tcttgccact ttctattttg aggcgggcaa ccatactgtg ctgttgcagg acaatgccac
     1381 ctatccttct ccaggagtgg ttattgctga tgcgataagg tggtcttgga gcagtccac
//
LOCUS       DAOQUC010001230         1437 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_125080_length_1437_cov_3.929088, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001230 DAOQUC010000000
VERSION     DAOQUC010001230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1437
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125080_length_1437_cov_3.929088"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..795
                     /locus_tag="PKH07_17150"
     CDS             <1..795
                     /locus_tag="PKH07_17150"
                     /inference="COORDINATES: protein motif:HMM:NF014196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliH/SctL family protein"
                     /protein_id="HNT36718.1"
                     /translation="HAEAKRISLSNRSKVLKDTLLHPQPVLVGERSVERLPDGMFSRE
                     DLLRQAQATLQQAQKEKQTILSNALAEKERILNQARLERDEIQRRAYEEGYEKGLQEG
                     LNKGTQQGRQEVLKVQKPIGDRLQGIVDAAEQAFSRVCTECEHDMIELIISVAEKVIH
                     QQIDQSNEIVLNMVRETIARSADKREVLLRVNPDDVELVREHQEVLSAEFDDMRVLRL
                     EPDPRITRGGCLLETSSGCVDGRVEAQLAETRKAFRPHPLKTHADV"
     gene            821..>1437
                     /locus_tag="PKH07_17155"
     CDS             821..>1437
                     /locus_tag="PKH07_17155"
                     /inference="COORDINATES: protein motif:HMM:NF014873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36719.1"
                     /translation="MDRQASLLRKSNQMSYSSVPKINWDYYRERLLRTDPIKNNGRIT
                     QVVGLVLESVGPAASVGELCHLCPEGGQAIPAEIVGFRGDRVLLMPLADTRGIRPGCQ
                     VIATGAPHLVRVGDGLLGRILNGLGEFMDDGPSIIADAEYPVYNTPPHPLRRQRICEP
                     IQTGIKAIDGLMTCGKGQRVGIFSGSGVGKRLLPTPLPDKMPTRCP"
BASE COUNT          346 a          361 c          424 g          306 t
ORIGIN      
        1 cacgcggagg cgaagaggat atccttgtct aatcgaagca aagttcttaa ggacacactt
       61 ctgcatcctc agccggttct tgtgggtgaa aggagtgtcg aacgattgcc cgatggcatg
      121 ttcagtcgag aggatctcct tcggcaggca caggccacgc ttcaacaggc gcagaaggaa
      181 aaacagacga ttctttcaaa cgccctggcg gagaaggaac gaatcctgaa ccaagctcgt
      241 ttggagcgtg atgagattca gcgccgcgcc tatgaggaag gctatgagaa gggacttcaa
      301 gaaggtctga acaagggcac gcaacaggga aggcaggaag tcctcaaggt tcagaaaccc
      361 atcggtgatc gacttcaggg catagtagat gcggcggaac aggccttctc gcgcgtctgc
      421 accgaatgtg agcacgacat gatcgaactg atcatctccg tagcagagaa ggttattcac
      481 cagcagatcg atcaatccaa cgaaatcgtc ctgaatatgg tacgggagac cattgcacgc
      541 tcggcagaca agagggaagt actgcttcga gtgaatcctg acgacgtaga acttgtgcga
      601 gaacatcaag aagtgctcag tgccgaattt gacgacatgc gagttcttcg cttggaaccc
      661 gatcctcgaa tcacacgggg agggtgcttg ctggagacgt cgtcgggctg tgtggatggg
      721 cgtgtggaag cacaattggc tgagacgcga aaggcttttc gaccccatcc cttaaagact
      781 catgcagatg tctgacgagt gaacgtgtcg gcaggagtct gtggatcgtc aagcgagtct
      841 tcttaggaaa tcgaatcaga tgtcatacag cagcgttccc aaaatcaact gggattacta
      901 tcgagaaaga cttctgcgaa ccgatcccat caagaacaac ggtcggatca cccaagtggt
      961 aggtcttgtc cttgaatcag tcgggccggc tgcttctgtg ggtgagttgt gtcacctttg
     1021 tcctgaaggc gggcaggcca ttcctgcgga gatcgtgggt tttcggggtg acagagtcct
     1081 gctcatgcct ttggcggata cgcggggcat tcgacctgga tgtcaggtca ttgcgaccgg
     1141 agccccccat ctggttcgag tcggtgacgg ccttcttggg cgaattctca acggcttggg
     1201 ggagtttatg gacgacggtc cctcgatcat tgccgatgcc gagtatcccg tctacaacac
     1261 gccgcctcat cccctgcggc ggcagcgaat ctgtgaaccc attcagacag gtatcaaggc
     1321 aattgatggc ctcatgacct gtggcaaggg acagcgcgtt ggcatctttt ccggcagtgg
     1381 tgttggcaag agactcttgc caacaccact gccggacaag atgccaacgc gctgtcc
//
LOCUS       DAOQUC010001231         1436 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_125264_length_1436_cov_2.897900, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001231 DAOQUC010000000
VERSION     DAOQUC010001231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1436
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125264_length_1436_cov_2.897900"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..745)
                     /locus_tag="PKH07_17160"
     CDS             complement(<1..745)
                     /locus_tag="PKH07_17160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36720.1"
                     /translation="MPPTPAEEKGKNLLYFLSPAIAALVFFLYALRPGYIFFWYDTSL
                     IHLPLKQFFHNSIASGDFPTWARHFLFGFPILGHGDAAYLYPLSAVLFSLFEPTRIYN
                     VFIVSHYLLAGLFCYCFLRALAVRRESAAFGAIIYALNGFSLGHLHHATILCGYAYLP
                     LLLWAVEIAARGNPFRGALIWALGLWLVTTASQPQMLLYCVCVSAAFWLWRALSQEKS
                     NRLLPTRILAMLVGTAAGAMLAALHIIPFL"
     gene            complement(726..>1436)
                     /locus_tag="PKH07_17165"
     CDS             complement(726..>1436)
                     /locus_tag="PKH07_17165"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNT36721.1"
                     /translation="RQYQVATWARMHGWDKDRAFQFVSNFRRNKVLAMACLETVQVEP
                     PTQQQAREFFDNNPELFQIPVEYDYLEFYVAAFKEKDYPSPADVFLANEGLKQAFSTV
                     HQRVLAGERVEDVSGEFANLKGRFWVLPRTSVSEKEQPQVMNDAFAALSIGEYSSVFE
                     TQRGFHFVQLERRSERSAMDWQQAEQQALKHCEMNARYQAQQSLIEEAYTSSAPTLNE
                     KAIARFEEEVVPSAPNSR"
BASE COUNT          378 a          372 c          373 g          313 t
ORIGIN      
        1 ccagaaatgg gatgatgtgt aaagctgcaa gcatggcacc tgccgcagtt ccgaccagca
       61 tcgcgagaat acgagttggc agaagccgat tggacttttc ctgactcaat gctctccaga
      121 gccagaatgc agcgctcaca cagacacaat agagaagcat ctgaggctgg cttgctgtcg
      181 tgacgagcca cagccccaac gcccagatta gtgcacctcg aaagggattt ccacgcgccg
      241 caatctcaac tgcccagagc agcaaaggca ggtaggcata gccacagaga atggtggcat
      301 ggtggagatg accaagactg aaaccattca gcgcatagat gatggctcca aaagctgcag
      361 attcacgacg aacagcaaga gcgcgaagaa aacagtaaca gaaaagaccg gcaagaagat
      421 aatgcgacac gatgaagaca ttgtagattc ttgttggctc gaacagggag aacaagactg
      481 cggacagtgg atagagatag gcggcatctc catgtccgag gattgggaat ccaaagagaa
      541 agtgacgtgc ccatgtcggg aagtcgcccg atgcgatgct attgtggaaa aactgcttca
      601 ggggcagatg aattagactg gtgtcatacc agaagaaaat ataccctggt cttagcgcat
      661 acaggaaaaa caccaatgcc gcgattgcgg gggacaggaa gtagagcagg ttctttcctt
      721 tttcctcagc gggagttggg ggcactcggg acaacctctt cctcgaatcg agcaatggct
      781 ttctcgttca acgtcggagc gctactcgta taggcttcct cgataaggga ttgctgtgcc
      841 tgatagcgag cattcatctc gcaatgcttc aatgcctgtt gctcggcttg ttgccagtcc
      901 atcgcactgc gttccgagcg tctttcgagt tgcacaaagt gaaaaccgcg ttgcgtctcg
      961 aaaacgctgg agtactctcc aatgctcagc gcagcgaagg catcattcat cacttgtggc
     1021 tgttccttct cggacactga tgttctcgga agaacccaga accttccttt caaattcgcg
     1081 aattccccgc tgacgtcttc taccctctct ccggcaagca ctctctggtg cacggtacta
     1141 aacgcttgct tcaaaccttc atttgccaga aacacatctg cgggagacgg atagtctttc
     1201 tctttgaagg cagccacgta gaactccaga tagtcgtact cgactggaat ctgaaaaagc
     1261 tccgggttgt tgtcgaaaaa ctccctggct tgctgctggg taggtggttc gacctgcaca
     1321 gtttccaggc aagccatcgc aagaaccttg tttcgacgga agttcgaaac aaattggaaa
     1381 gcccgatcct tgtcccaccc atgcatccgt gcccatgtag caacttggta ttgccg
//
LOCUS       DAOQUC010001232         1436 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_125309_length_1436_cov_2.302679, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001232 DAOQUC010000000
VERSION     DAOQUC010001232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1436
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125309_length_1436_cov_2.302679"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..348)
                     /locus_tag="PKH07_17170"
     CDS             complement(<1..348)
                     /locus_tag="PKH07_17170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011735830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HNT36722.1"
                     /translation="MKIAFSTSGKSLDALLDQRFGRAAGFLVYDLDANTYEVMDNKVN
                     LNAAQGAGIKSAETVVRAGAKAVVTGHCGPKAFRALAAAGVQIYNTGAATVAEALEQY
                     RAGKLEQSQSPDVE"
     gene            complement(612..1016)
                     /locus_tag="PKH07_17175"
     CDS             complement(612..1016)
                     /locus_tag="PKH07_17175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011026051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5320 domain-containing protein"
                     /protein_id="HNT36723.1"
                     /translation="MPRGDGTGPLGQGPMTGRGAGFCVGYAMPGYANPMPRRGFGMGY
                     GSGFGGRGSGGRGRGFRHMYYATGVPGWARFGGRTAPPMNPGGYQAFEPMESEQEILS
                     RQAEALHSELDSIKKRLAEIEQSKTEAKPAAE"
     gene            complement(1125..>1436)
                     /locus_tag="PKH07_17180"
     CDS             complement(1125..>1436)
                     /locus_tag="PKH07_17180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="dinitrogenase iron-molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HNT36724.1"
                     /translation="SFVAGDVNEVIQAWLSGKLGTDTYAMPGCGCQRGGRGWGSGGWR
                     GGNARGAVGAESGTAFLQDNGQQECICPACGYRAPHQRGVPCSRLRCPQCGAALRRGF
                     S"
BASE COUNT          330 a          443 c          337 g          326 t
ORIGIN      
        1 ttcgacatcc ggagactggg actgttcgag ttttcctgcc cgatactgtt ccaatgcctc
       61 agcaacagtg gctgcgcctg tgttgtatat ctgaacacct gcggcggcaa gcgcccgaaa
      121 tgctttggga ccacagtggc cggtcactac ggccttcgcc ccagcgcgaa ccaccgtttc
      181 tgccgacttg attcctgcgc cctgagccgc attcaggttg actttgttgt ccataacttc
      241 atacgtattg gcatcaagat catagacaag aaatcctgct gcacggccaa aacgttgatc
      301 gagcagagcg tccagcgatt tacctgaagt ggaaaacgca atcttcattc tgatctcctt
      361 agaaaaacaa gatagcctcc agttgagatt gagtcaaaga gacccaaacc atgcatcgag
      421 catgtgtcca agaggcaatt cataactcac tctgttgctg gcgctcgcct gcggggcgag
      481 actgtccctg ttctgtacaa aatccgaagc caccttggag cctgaagcac cacagcgtgg
      541 ctgcttcgga cataccgtgt ggcggttgcg aaagtcaaga gatctgacgc aaccgccaca
      601 aaatgacgcg attactcggc tgcaggcttc gcctctgtct tgctctgttc gatttctgca
      661 aggcgtttct taatggaatc aagttcagaa tgcaaagcct ccgcttggcg acttagaatc
      721 tcttgttcgg actccattgg ctcaaaagcc tgatagccac cgggattcat tggcggtgct
      781 gtccttccgc caaaacgagc ccaaccggga acacccgtcg catagtacat gtgtcgaaag
      841 cctcgacctc gaccacccga accgcgtccg ccaaagccgc ttccatagcc catgccgaat
      901 ccgcgccttg gcattggatt ggcatagcct ggcattgcat agccgacgca gaatcctgca
      961 cctcgacccg tcatgggacc ctgtcccaag ggacctgttc catctcctct tggcatgatt
     1021 ttgcctcctt tcacgctggc aactgccagc agacctgtgg gagataccca ctctgatgaa
     1081 atccttgttt cataccgaaa atccgttctt ttctattctg tcattcagga gaatcctctc
     1141 cgaagtgctg caccacactg aggacagcgc agcctgctgc atggaacacc gcgctggtga
     1201 ggtgcacgat agccacatgc gggacagata cattcctgct gtccattgtc ctgcaggaaa
     1261 gcagttccag attcagcacc gaccgcaccg cgagcgttgc caccccgcca accacccgaa
     1321 ccccagcctc ttcctcctct ctgacaacca caccccggca tggcataagt atccgttcca
     1381 agttttcctg agagccatgc ttgaatgacc tcattgacat cgccagcgac aaagga
//
LOCUS       DAOQUC010001233         1434 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_125559_length_1434_cov_3.047136, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001233 DAOQUC010000000
VERSION     DAOQUC010001233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1434
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125559_length_1434_cov_3.047136"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(5..670)
                     /locus_tag="PKH07_17185"
     CDS             complement(5..670)
                     /locus_tag="PKH07_17185"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNT36725.1"
                     /translation="MCQTTAVSKEGIRLNLQDITKRYPGVLALDRVSFALEMGRVHAL
                     VGENGAGKSTLIKILAGVLHPDEGRIVHQEADISISSPQRARQIGISVVHQHSHLIPN
                     LSIAENYALRVAYPRTSLGTISWGTVSHQAAVATQILIPSLDVQRLARSLSGGERQLV
                     ELSFAFASSPDVMILDEPTAVLPERETGLLFQRIKEFAHAGGTVLFVSHRLEENANSS
                     LFR"
     gene            748..>1434
                     /locus_tag="PKH07_17190"
     CDS             748..>1434
                     /locus_tag="PKH07_17190"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNT36726.1"
                     /translation="MRITIKDIARMGSTSIATVSRVLNGKPGVSQITRLRIQEIIDQT
                     GYRPSLVAQNLALRKSLHLGFVASNLVNSFYVDLFRQIEKLTRNEGYHLLLMDSEQDI
                     EHERENIKLMRQHQVQGLIIVPVHDYDESTDVDHLLRLKLSRFPFVIFGKVDGYGFDC
                     VTTEERDAAAQLTRHLLDYGHRRFAFVGDHPNNRCVRERIQGVRDALAERGLHLPQQA
                     AIPMDDIWFDR"
BASE COUNT          353 a          386 c          364 g          331 t
ORIGIN      
        1 acttttaccg gaacaagctg gaatttgcat tttcttccag gcgatggctc acaaagagaa
       61 cagtcccccc agcatgagcg aattctttga tcctctgaaa aagcagtcct gtttcacgtt
      121 caggcagcac ggcggttggc tcatcgagaa tcatgacgtc cggactggaa gcaaatgcga
      181 aggagagttc caccagttgc ctctctcccc cgctgagaga acgcgccaaa cgctgaacgt
      241 ccaatgaggg aatcaggatt tgtgtcgcta cggcagcctg gtggctcaca gttccccagg
      301 agatcgttcc cagagacgtc cgtggatagg ccacacgcaa agcatagttc tctgcaatcg
      361 agagattcgg aatgagatgg gaatgctgat gcacgacgga gattccaatc tgtcgtgccc
      421 gttgggggct gctgatggat atatccgctt cctggtgaac gattcgacct tcatcaggat
      481 gcagcactcc ggccaggatt ttgatcaggg ttgacttgcc cgccccattc tcaccgacaa
      541 gtgcatgcac ccgtcccatt tcaagggcga agctcacccg atcgagcgcg agcacgccag
      601 gatagcgctt ggtgatatcc tgaagattga gccgtatccc ctccttggag actgctgttg
      661 tttggcacat accagttgat cacactcatt ttccagagta tagttcaaaa atatgattat
      721 cacagtccag taaataagga aagatctatg cggatcacaa tcaaggatat cgcccgcatg
      781 gggagtacct caatcgccac ggtttcccgg gttctcaatg gcaagccagg tgtcagtcag
      841 ataacccgat tgaggattca ggagatcatt gatcagacgg gatatcgtcc gagtcttgtt
      901 gcccagaatc tcgctctcag aaagagcctc caccttggtt ttgttgcatc caatcttgtc
      961 aactctttct atgttgatct tttcagacag atcgagaaac tcacccgaaa cgagggctat
     1021 catctccttc tgatggactc tgagcaggac atcgaacacg agagggagaa catcaagttg
     1081 atgcgacagc atcaggttca gggcttgatc attgtcccag tacatgacta cgatgagagc
     1141 actgatgtag accatctgct tcgtctcaaa ctcagcagat tccccttcgt catttttggc
     1201 aaggtggatg gatacggatt cgactgcgtc accacagaag agcgagatgc tgccgcgcaa
     1261 ctgacacgac accttctgga ctacggccac cggcgatttg ccttcgtcgg cgatcacccc
     1321 aacaaccgct gcgttcggga gcgcatacag ggcgtcagag acgcactcgc agaacgtggc
     1381 ctgcacctgc cacagcaagc agcgatcccc atggatgata tctggtttga tcgt
//
LOCUS       DAOQUC010001234         1433 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_125774_length_1433_cov_2.145138, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001234 DAOQUC010000000
VERSION     DAOQUC010001234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1433
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125774_length_1433_cov_2.145138"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="PKH07_17195"
     CDS             <1..238
                     /locus_tag="PKH07_17195"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HNT36727.1"
                     /translation="IARAARYLAAGIAAVVCVLDPEMVVIGGGGANMGDVLLDPVREE
                     VKRRVYFSDAVDIPIVCAQLGEDAGFVGAAGLLF"
     gene            262..>1433
                     /gene="ispH"
                     /locus_tag="PKH07_17200"
     CDS             262..>1433
                     /gene="ispH"
                     /locus_tag="PKH07_17200"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HNT36728.1"
                     /translation="MEVIQAQTAGFCMGVRRAVDMVLEVSKRPTGRIVTYGPLIHNPQ
                     VLELLKIKGVEAISNPSDSAGATVVVRAHGISPQVRTTLQASSASVCDATCPHVLRAQ
                     HVIQKHASKGFFAVIVGDEGHAEVEGLLGCAGVNGAVVGSASEVETLPKDLDAVCVVA
                     QTTQNQETFDSVCQALRERYSQVKIFNTICSATHDRQEEVRSISKQVDAMVIVGGRNS
                     ANTLRLAEIAQANGVQTFLVESEEELDLETLSKFKKVGITAGASTPYWMIDRVVETVK
                     ALPMEKRGRAFLAARELLLILLKGNLMTALGAASVSYTIAALLFEKFDTIFCTIAATY
                     VLSMHIFNSYADRHSAMFNEPFMARFYSRYGFGLQSLGVIAAAISLTLAGFQGELT"
BASE COUNT          305 a          371 c          419 g          338 t
ORIGIN      
        1 cattgcaaga gcagctcgtt atctggcggc agggattgca gcggtggtct gtgttctgga
       61 tccggaaatg gtggtcatag gtggcggtgg agccaacatg ggggacgtgc ttctggatcc
      121 tgttcgtgaa gaagttaagc gcagagtcta cttttctgac gcggtggaca tcccaatcgt
      181 ctgtgctcag ttgggagagg acgcaggttt tgttggagca gcaggtttgc tgttctagtc
      241 agtcgcaatg gagagaattc catggaagtc atccaggcgc agacggcggg tttctgtatg
      301 ggggttcgcc gtgccgtgga tatggtgctg gaagtctcca agagaccaac cggccgcatc
      361 gtgacctacg gcccactgat ccacaatcct caggtcttgg aactgctcaa gatcaagggc
      421 gtagaggcca tctccaatcc atccgattct gctggggcaa ccgttgtggt acgcgctcat
      481 ggcatatcac cacaggttcg caccacattg caggcatcca gcgcatcggt ttgtgatgcg
      541 acctgtcctc atgtactgag agcgcagcac gtcattcaga aacacgcttc caagggattc
      601 ttcgcggtta tcgtcggaga tgagggtcat gcagaagttg agggtctgct cggctgcgct
      661 ggtgtcaacg gtgccgtggt aggaagtgct tcggaggtcg agacacttcc caaagatctg
      721 gatgctgttt gtgtcgttgc acaaacgacg cagaaccagg aaactttcga ctcggtctgc
      781 caggctttgc gggagcgcta ttcacaagtc aagatattca ataccatctg cagcgccacg
      841 catgaccgtc aggaagaggt gcgctccatc tccaagcagg tggatgccat ggtcatcgtc
      901 ggtggacgca acagtgccaa taccctgcgc ctggcggaga tagcccaggc aaacggggtg
      961 cagacttttc tcgtcgaaag tgaggaagaa ctggatcttg agaccctttc aaagttcaag
     1021 aaggtcggca tcacagcggg cgcttccacc ccgtactgga tgatcgaccg ggtggtcgaa
     1081 acggtcaaag cgcttcccat ggagaaacgg ggaagagcgt tcctggcagc acgagaactg
     1141 ctgttgatac tccttaaggg aaacctgatg actgccttgg gagcagcttc ggtttcctac
     1201 accatcgctg cgctgttgtt tgagaagttc gataccatct tctgcacgat cgctgccaca
     1261 tacgttctct ccatgcacat cttcaacagc tacgccgacc gccattcagc aatgttcaat
     1321 gagcccttca tggctcgatt ctacagccgc tatggctttg ggcttcagtc tcttggtgtc
     1381 atcgccgcgg cgatttcgct cacactggct ggttttcaag gtgagctgac ctt
//
LOCUS       DAOQUC010001235         1433 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_125788_length_1433_cov_1.952830, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001235 DAOQUC010000000
VERSION     DAOQUC010001235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1433
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125788_length_1433_cov_1.952830"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1167)
                     /locus_tag="PKH07_17205"
     CDS             complement(<1..1167)
                     /locus_tag="PKH07_17205"
                     /inference="COORDINATES: protein motif:HMM:NF015859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE domain-containing protein"
                     /protein_id="HNT36729.1"
                     /translation="MALLIRSKRLKWALALLVPVVLLGTVYAWNCVRDADRQMREDHL
                     RSARLIAQAFNVQVLLSLSGTEADLSLSEYLRLKNYLAASRTLVRGCRFVYLMGRKPD
                     GKIFFYADSEPVGSADESPAGQIYEEASEELIATFSTQTEAVEGPVADRWGTWVSAQV
                     PIQDPKTGRLVAVLGVDIAADDWQWMVARASSVPIVLSLILAGFVVGGQMLLSWRDRL
                     GAEHSPWLGHIEAWLAAMIGLVLTFAVVWQAHETDKRNRAEVFAQLASTKTAHVLESL
                     KRLRDVEVEGLAQFFEGSEDVTPQEFHAYARHLTKNPAIQAWLWVEAVPRENKEAFEQ
                     RSKQRGLSDYEIWESDSDGKKIRVLDRETYYPVLYVAPTEQNDRGLGYDLGSDPT"
BASE COUNT          349 a          424 c          369 g          291 t
ORIGIN      
        1 tgtggggtct gatccaagat cgtaacccaa acctctgtca ttctgttcgg tgggcgcaac
       61 gtagagcacc ggatagtatg tttctcggtc aagaacacga atcttctttc cgtcggaatc
      121 gctctcccag atctcatagt cagacaggcc ccgttgcttg gagcgttgct cgaaggcttc
      181 tttgttttca cggggaacag cctcaaccca gagccatgcc tggatggctg ggtttttcgt
      241 cagatgcctg gcgtatgcgt gaaactcctg tggagtcaca tcctcgctgc cctcaaagaa
      301 ctgcgccaaa ccctcaactt ccacatcccg aagtctcttg agggattcaa gaacatgggc
      361 cgtcttggtg cttgcaagtt gagcaaatac ctcagcacga ttgcgcttgt cggtctcatg
      421 cgcctgccag acgactgcaa aagtcaagac caacccgatc atggcggcaa gccacgcttc
      481 aatgtgcccc aaccatggtg aatgttctgc cccaaggcga tcgcgccagg acagaagcat
      541 ctgaccaccg acgacaaatc cggccagaat aagactgagc acgatgggca cagaagaggc
      601 gcgtgcaacc atccactgcc agtcgtccgc cgcaatatcc acacccagga ctgcaacaag
      661 cctgcccgtc ttcggatcct gaattggaac ctgagcgcta acccacgttc cccagcggtc
      721 tgcgacggga ccctccacgg cttctgtctg cgttgagaaa gtggcgataa gctcttcgga
      781 tgcctcttca tatatctgac ctgcaggaga ctcatcggcg gatccgaccg gctctgaatc
      841 ggcgtagaag aatatcttcc cgtcaggctt gcgacccatc aggtagacaa atcggcatcc
      901 gcgcaccagc gttcgagacg cagcaaggta attcttgaga cgaagatact ccgataaaga
      961 aaggtctgcc tcagtacccg aaagactgag caagacttgg acattgaacg cctgcgcgat
     1021 cagtctcgcg cttcggagat gatcttcacg catctggcga tctgcatctc ggacacaatt
     1081 ccatgcgtac acagtgccca acagcacaac aggcaccagt agagccagcg cccacttcag
     1141 tctcttgctt cggatcaaaa gagccacacg cctttctcca aactgcgtca catctcatca
     1201 gcgcagtgac atccgagggc gagtgcagcc cacaaagatg aggggatgac ccgctccaaa
     1261 ctctcggtga cagatcccag aagaccaggt caatccagat gataatccgc cttcaagtca
     1321 aagtaaagac gatgtgtact ggctcagtca tggaggggtg gaggtatgct tcggttgcag
     1381 aggctctgcg acgctgccga agcgggaaca gccagacagc gggcttcggt agc
//
LOCUS       DAOQUC010001236         1432 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_125850_length_1432_cov_3.294118, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001236 DAOQUC010000000
VERSION     DAOQUC010001236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1432
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125850_length_1432_cov_3.294118"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1191
                     /locus_tag="PKH07_17210"
     CDS             <1..1191
                     /locus_tag="PKH07_17210"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HNT36730.1"
                     /translation="VLLDELGAGTDPIEGGALGIAILDELRVRKATTLVTTHLDDLKV
                     YAHLTEGVINAAMQFDLETLQPTYRLTLGIAGESNALRIASRLGMPAALTDRAEAELA
                     GRETEEAGALYRRLVSEVQSAHQDRQKASQERNQVTRLAEEHRKMVQKLEKERHDILH
                     RARIQAQRMVEDARRKVKDAEREFEIQRQLLVQQQEKNLTLEPVRQALDQTEAELGLE
                     QEEPLLQEPEPVPIDLEALRAGDMVQVAGFMKPGKVLKIDRERGEVLLTLSSWEMTLP
                     LARLAKMVRALSPSEKEQVERQQKKTPQTSWAYEVKESPGMEIHLRQMRVHEGLRLLD
                     RYLEDAFMAGLDEVRVIHGIGTGAMLRAVHEALRDNHLVKKFRLGEPSEGGGGATIVT
                     FRER"
BASE COUNT          361 a          372 c          434 g          265 t
ORIGIN      
        1 gtactgctgg atgaactggg cgccggcacc gatcccatcg agggaggagc gcttggcatc
       61 gctattcttg atgaactgcg agtacgaaag gcgacaacgc tggtgacgac ccacctggac
      121 gatctcaagg tctatgccca tctgacggaa ggcgtcatca acgccgcaat gcagttcgat
      181 ctcgagactc ttcagcccac ttaccggttg accctcggca tcgcgggaga aagcaatgcc
      241 ctgcggatcg cctcaaggct cggaatgcct gctgcgctca ccgatcgggc agaggccgag
      301 ttggcagggc gggaaaccga agaggctggt gccttgtacc gacgcctggt ctcagaagtt
      361 cagtccgccc atcaggatcg ccagaaagcc tctcaggagc gcaaccaagt tacccgactg
      421 gcggaagaac accgaaagat ggtgcagaaa ctggagaagg aacgacacga catcctgcat
      481 cgcgcccgca ttcaggcaca aaggatggtc gaagacgctc gtcgcaaggt gaaggatgct
      541 gagcgggagt ttgagattca gagacaactc ctggtacaac agcaagagaa gaaccttacc
      601 ttggaacctg tccggcaggc gcttgatcag actgaagcgg aactcggctt ggaacaggag
      661 gaacctctgc ttcaggaacc tgaaccggtt cccattgatc tggaagccct cagggcgggt
      721 gacatggttc aggtcgcagg cttcatgaag cctgggaagg ttctcaaaat tgaccgagag
      781 cgcggcgagg tgttgctgac cctcagttcc tgggagatga cgctgccact ggcgcgtctg
      841 gcaaaaatgg ttcgcgcgct ttccccttcc gagaaagaac aggtggagcg gcagcagaaa
      901 aagactcccc aaaccagttg ggcgtatgaa gtcaaagaga gcccaggcat ggagatccac
      961 ctcagacaga tgcgcgtcca tgaagggctt cgattgctgg atcgctatct tgaggatgca
     1021 ttcatggctg gcttggatga agttcgggtc atccatggta tcggcacagg agcgatgttg
     1081 cgtgccgttc atgaggcact tcgggacaat caccttgtga agaaattccg actgggcgaa
     1141 ccctctgaag gtgggggcgg ggcaaccatc gttactttca gagagcgctg atacgtgtat
     1201 caagaaagaa tgtgtccgcg ttcagtcaac gaatggatga ccgtttcaac tgaacgaggc
     1261 cgccaaaacc gaatacagac aggagcggag ctccaaaatt ctcacgctgg agaagacgat
     1321 gacacagaag gttctttccg catcgcagac agaatcgcag cgaacggtaa ctgattgggg
     1381 ttgcttgcga tggcttgctg acggcaagaa gggcaacgct caaggactca ca
//
LOCUS       DAOQUC010001237         1431 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_126011_length_1431_cov_3.313953, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001237 DAOQUC010000000
VERSION     DAOQUC010001237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1431
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126011_length_1431_cov_3.313953"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..665
                     /locus_tag="PKH07_17215"
     CDS             <1..665
                     /locus_tag="PKH07_17215"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNT36731.1"
                     /translation="PFKLKRPVRLFHGGVDCEKFTPGRTGAGIRAEFAVGEEDCLICA
                     VGHLARVKGYETLLEALELARRANPAIKCLIVGMGSEEYRSSLQEQIEQLGLQEAVTL
                     TGFRSDVAEIMAASNFGVLSSVSSEGNSRTALELMACGLAIVATSVGCLPDLVLDNET
                     GYIIPPGDPASMAEKILVLSEHPALGAAMGEKGRERVLANYSEQTVAEEMEMLYREII
                     GL"
     gene            930..>1431
                     /locus_tag="PKH07_17220"
     CDS             930..>1431
                     /locus_tag="PKH07_17220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014448953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNT36732.1"
                     /translation="MFPLERIRNFSIIAHIDHGKSTLADRILEYTNSIPDRDMRDQVL
                     DTMDLERERGITIKAQTARFFYHARDGQEYLLNLIDTPGHVDFTFEVNRSLVACEGVL
                     LVVDASQGVEAQTIANLYKALDLNLEVIPVINKIDLPGADVVNTMAQIHDILGAEEHE
                     MLTSSAK"
BASE COUNT          330 a          350 c          409 g          342 t
ORIGIN      
        1 ctcccttcaa actgaaacgc cccgttcgtc tgtttcatgg gggcgtggac tgcgagaagt
       61 tcactccagg acgcacgggt gctgggatac gtgccgagtt tgccgtcggg gaagaagact
      121 gtctgatatg tgctgttgga catcttgcac gagtcaaggg atatgaaacg ctgctcgagg
      181 cgctcgaact ggcgcgaaga gccaatcccg cgatcaagtg tctgatcgtt gggatgggga
      241 gtgaggagta tcggagcagt ctgcaagagc agatcgaaca acttgggttg caggaggctg
      301 tcacattgac aggttttcga tccgacgttg cggagatcat ggctgcgtcc aatttcgggg
      361 ttctttcttc agtcagttct gagggcaata gccgtacagc actggaactc atggcttgcg
      421 gcttggcaat tgttgccaca tcggtcggat gtctgcccga tctggttctg gacaatgaaa
      481 cgggctacat catcccacct ggagatcccg cctcgatggc cgagaagatt ctcgttctgt
      541 cggaacatcc agcgcttggt gctgccatgg gtgagaaggg acgcgagcga gtcctggcca
      601 actactccga gcagacagtg gcggaggaaa tggaaatgct ctacagggag atcattggcc
      661 tgtaatccgc attggccgaa cgaaacgagg aatctctttt ctcaaccccc atttccctat
      721 gcaatgacag ttggcactgt actttgtcta tcgcgaatct ctctctttga atctgtcgtt
      781 acctcaacag gcaaattctg ctatatagga gtgctgcggt ttcaggtgaa ctcattgttc
      841 gcctggaaca ggacggactg ttcgctatca gttaagagtg tccgggagag gaacggtttg
      901 tctttgaaca cgaattggca ggagattgta tgtttccgct agaacggatt cggaattttt
      961 ccatcattgc acacattgat cacggcaagt ccacacttgc cgaccggatt ctcgaataca
     1021 ctaacagcat tccagaccgc gacatgcgcg accaggtgct ggatacgatg gatctggaac
     1081 gcgaacgcgg gatcacaatc aaagcgcaga cggctcggtt tttttaccac gctcgagacg
     1141 ggcaggaata tctgctgaat ctcatagata ccccaggaca cgtggatttt acctttgagg
     1201 tgaatcgcag cctggtggcc tgtgaagggg ttcttctcgt tgtggatgcc tcgcagggag
     1261 ttgaggcgca gaccatcgcc aacctctaca aggcactcga tctgaatctg gaagtgatcc
     1321 cggtgatcaa caagatcgat ctgcccggcg ctgatgttgt gaacacgatg gctcagattc
     1381 atgacatttt gggtgccgaa gagcacgaga tgctcacaag cagtgcgaaa c
//
LOCUS       DAOQUC010001238         1431 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_126029_length_1431_cov_3.055959, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001238 DAOQUC010000000
VERSION     DAOQUC010001238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1431
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126029_length_1431_cov_3.055959"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            282..902
                     /locus_tag="PKH07_17225"
     CDS             282..902
                     /locus_tag="PKH07_17225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011737177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HNT36733.1"
                     /translation="MCHRYLRRILLGVCVLITSGCQPRASEFRDGDIIFHTSRSAQSE
                     AVQRATRSKYSHMGIIFFKEDEPYVFEAVSPVKFTSLQKWIDRGKNGRYVVKRLVNAD
                     AVMSAAGLKKLRDSARQFEGKPYDLTFEWSDDRIYCSELVWKMYDRALGIEVGRLQKL
                     REFDLSEPLVKQKVGERYGARVPLDETVISPQAIFDSPNLVTIDRR"
     gene            994..1269
                     /locus_tag="PKH07_17230"
     CDS             994..1269
                     /locus_tag="PKH07_17230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36734.1"
                     /translation="MIKRILSAIIAIIYLSVAYLGGGSEGAIRCALFLFLPMACIWFS
                     EEMGSFTGVMRGHYINAETPGCLVALGGWFVLMLPFIIWIIEHFSAK"
BASE COUNT          347 a          339 c          400 g          345 t
ORIGIN      
        1 tcgaacaaca gcctgctctc gtattgctca cccgcgtgga cgcgggctcg caaacgtaga
       61 ggccggacgt tcgggcagcc aaggagccta gtgcatgatt cggcctccgc tacgcctaaa
      121 agacctggaa ctttgccagt tccaaggtct aagtgaggca agcaacgctc caaggcaagt
      181 ggcaagtatc tcggttctct cgaagacagg atcagatctg caccggaatc agaggtacct
      241 gagctgagtg catcctgagc tgcgcactga ggagttatgt tatgtgtcat aggtacttga
      301 gacggattct tctaggtgtc tgcgtcttga tcacttccgg gtgtcagcca cgcgcatctg
      361 agtttaggga cggtgacatc atattccata cgtcccgttc agcacagagt gaagcagtgc
      421 agcgcgccac ccgttccaag tacagccaca tgggcatcat ctttttcaaa gaagatgaac
      481 cctatgtttt tgaggccgtc agtccggtga agttcacatc tcttcagaag tggattgatc
      541 ggggcaaaaa cggtagatac gtcgtgaagc gccttgtcaa tgcggatgct gtcatgtcag
      601 ctgcaggcct caagaagctg cgggattccg cccggcagtt tgagggtaaa ccctacgact
      661 tgacgttcga gtggtcggac gatcgaatct attgttcaga actcgtctgg aagatgtacg
      721 atcgagcgct tgggattgag gtaggcagac ttcaaaagtt acgcgaattc gatttgtcgg
      781 aaccactagt gaaacagaag gttggagaac ggtatggagc gcgggttcct ttggatgaga
      841 ccgtcatatc cccacaggca atattcgatt caccaaatct cgttacgata gacaggcgtt
      901 gagtatgaca ctcaacgaga cgctgcaagt agaaaaccca agctcttcgc gctcgttctt
      961 gcgcttgaac tccaacgttc gacaggagaa gaaatgatca agaggatact ctccgcgatc
     1021 attgcgatca tctacctgtc tgtggcctac ttgggtggag ggtcagaagg cgcaatcagg
     1081 tgcgccctgt ttcttttcct gccgatggcg tgtatctggt tcagtgaaga aatgggatct
     1141 ttcacaggtg taatgcgagg gcattacatc aatgcagaga caccaggttg tttagtcgcc
     1201 ttaggcggtt ggttcgttct gatgctgcct ttcatcatct ggatcattga gcacttctcg
     1261 gcaaaatgaa gatgaacagg aaggaatctc cagcaagaga gtggagtact acatttcaca
     1321 cagcgctgcg ccgtgtagaa caagggtcac gcttgtcgtt tgtgtggaaa gagattgctg
     1381 cagattcaga cggctgctgg actgcgctac ccaattgaag gcagtcttgg g
//
LOCUS       DAOQUC010001239         1431 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_126061_length_1431_cov_2.461483, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001239 DAOQUC010000000
VERSION     DAOQUC010001239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1431
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126061_length_1431_cov_2.461483"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1431
                     /locus_tag="PKH07_17235"
     CDS             <1..>1431
                     /locus_tag="PKH07_17235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36735.1"
                     /translation="SSGGIGLYRDDGTRFGFLDSNDSPIALRQATWQVSSASRYLIGL
                     QPVYPEEVPDDDGIPDATAEMGPDYGDDWFVVIRTGENITLGDEFRFSIPDNGVDFRT
                     AELIPGVQNQYSWTANVPTFLSSTVAWNGSIAENSAATGVIGVRVGDIGSYSPVLQSI
                     NLDIFGSGITDVSDGSLGLNIDRQELLVFDHADTADWLVEFVTSEVQFRVQKTKVTEW
                     HRFYAGNINLKDVSIDPSTISFDPLFNERRITYEVVFSDSLSFILYRVLFDGTRTIGL
                     ASGNILTDFYYSAGGRRALWIRSEDWTGQTAVAGDRFRFSALATIDGFGAIGTDYRSN
                     TAEILLRSTAWLGREADLSEYFLLHVKNGQFTKNDLLPPATTGPSGVSLWQDVNGNGI
                     LDASDTQVQLTARPYLLGNGPYSVTLTPVGGISLPTLNTLDRRNDLLIAVQTAAGVGD
                     RVLFNVAIPDYSSGKGVVYSIGTCNE"
BASE COUNT          302 a          364 c          419 g          346 t
ORIGIN      
        1 gaagcagtgg tggcatcggg ctgtatcgtg atgatggaac gcggtttgga ttcctggact
       61 caaatgacag tccgattgcg ctgcgccagg cgacgtggca ggtcagtagt gccagccgct
      121 atctcatcgg acttcagccg gtctatccgg aagaagttcc tgacgatgac ggcattccgg
      181 atgccacggc cgagatggga cccgactatg gtgatgactg gttcgtcgtc atacgaacgg
      241 gtgagaatat cactctcggc gatgagttcc gattcagcat ccccgataac ggcgttgatt
      301 tccggacagc cgagctgatt cctggtgtgc agaaccagta cagctggacg gccaatgttc
      361 cgacatttct ttccagtacg gtggcttgga acggttcgat tgccgagaac tccgctgcca
      421 ctggagtcat cggtgttcgc gtgggtgaca tcgggagtta ttctcccgta cttcagagca
      481 tcaacctgga tatctttggc agtggcatca ctgacgtgtc agacggttcc ctcggtctga
      541 acattgaccg acaggaactc ctggtctttg atcatgccga tacggcagat tggcttgtcg
      601 agtttgtcac ttcggaagtt cagttccgag tccagaagac gaaggtgacc gagtggcatc
      661 ggttctatgc cggaaacatc aacctgaagg atgtctctat tgatccatct acgatcagtt
      721 ttgatccgct gttcaatgaa cgtcggatta cctatgaagt ggtcttctcc gactccttgt
      781 ctttcatcct gtaccgggtg ttgtttgatg ggactcgaac gattggtctc gcgtcgggca
      841 atatcctgac agacttctac tactcagcgg gtggacgccg tgcgctctgg attcgcagcg
      901 aagactggac aggtcagacg gcagttgctg gagaccgatt ccgattctca gcactggcga
      961 ccattgatgg atttggagcc atcggcacgg actaccgaag caacacagcg gagatcctgc
     1021 tccgatccac cgcctggctt ggcagggaag ccgatctgag tgagtacttc ttgctgcacg
     1081 tcaagaacgg gcagttcacc aagaatgatc tgctgccgcc ggcgacgact ggtcccagcg
     1141 gtgtatctct gtggcaggat gtgaacggta acggaattct ggatgcgagt gacacgcagg
     1201 tccaactgac cgccaggcct tatctgttgg gcaatgggcc atacagcgtc acactgacgc
     1261 ctgttggagg catcagtctg ccgactctga atactctgga tcgacgcaat gatctgttga
     1321 tcgcagttca aacggctgcg ggagttggtg acagggtgct gttcaatgtg gcgattcccg
     1381 actattcgag cgggaaggga gtcgtctact ctattggaac atgcaatgaa c
//
LOCUS       DAOQUC010001240         1430 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_126236_length_1430_cov_1.826909, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001240 DAOQUC010000000
VERSION     DAOQUC010001240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1430
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126236_length_1430_cov_1.826909"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            112..327
                     /locus_tag="PKH07_17240"
     CDS             112..327
                     /locus_tag="PKH07_17240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015220173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HNT36736.1"
                     /translation="MKLTAIIEREGDGYVSLCPELDIASQGNTVEQARDNLREALELF
                     FETASPEEVKQRLHGEIFITQVEVALG"
     gene            320..541
                     /locus_tag="PKH07_17245"
     CDS             320..541
                     /locus_tag="PKH07_17245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015170015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HNT36737.1"
                     /translation="MGKLRVLSGKEVCAILSRHEFAEIRRRGSHIIMQKRLAQGTITI
                     PVSDHNEIRIGTLQSIIRQSGLSRSEFEL"
     gene            631..1005
                     /locus_tag="PKH07_17250"
     CDS             631..1005
                     /locus_tag="PKH07_17250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014819756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36738.1"
                     /translation="MSKYWVVGASWGGTEHQDQKFVDQGYWMLGWKEEKQPDQYAKAS
                     EMKIGDRIAIKRMKGQGQTGIRIFHLGVIKGVILDADKVICTVDWVATDLQRDIEESR
                     GCFKSIHGPYEKDEWVEKVFCL"
BASE COUNT          401 a          287 c          405 g          337 t
ORIGIN      
        1 atctggctac gcatcagtcg catctggcgc ctcacagaca gtgattggta aatagagaag
       61 tctatggtaa ttttatacga agagcgatcc tagggagaca ggaggttacc aatgaaactt
      121 acagccatta ttgaacgcga gggtgacgga tatgtatcac tttgcccgga gttagatatt
      181 gcaagtcaag gaaacactgt cgaacaagcc cgggacaatt tgcgcgaagc attggagttg
      241 tttttcgaga ccgcatcacc cgaagaggtc aagcaacgtc ttcatggcga gatattcata
      301 acccaggttg aggtggcact tgggtaaact tcgagttctt tctggcaaag aggtgtgcgc
      361 catcttgtcc agacatgagt tcgcagaaat acgccgacgc ggtagccaca tcattatgca
      421 gaagagattg gcacaaggca cgataacaat tcctgtgtca gaccataacg aaatccgcat
      481 tggcacactg caatccatca ttcgtcagtc agggctgtct cggagtgaat ttgaattatg
      541 aacttttggc acctaacaac ggcatgcagc ggacggtggt gcgcaccgcc gctgatgctg
      601 agcgttatgt gctttaagga gtgaaaaacg atgtcgaagt attgggtcgt aggagcatcc
      661 tggggcggga ctgagcacca ggaccagaag ttcgtagacc aagggtattg gatgttgggt
      721 tggaaggaag aaaagcagcc tgatcaatat gcgaaagcgt cggagatgaa gatcggggac
      781 cgaatcgcca tcaagcgaat gaaagggcaa ggtcaaactg gcatccggat tttccatctt
      841 ggcgtgatta aaggcgtgat cctcgatgcg gataaggtca tctgtacagt cgattgggtg
      901 gctactgacc tgcaacgtga tattgaagaa agccgtgggt gtttcaaatc aattcatggt
      961 ccgtacgaga aagacgagtg ggtggagaag gtcttttgct tatagcggag tttacgcata
     1021 acacgtcgct ccagccgacg tcagtccgcg atgcggcctc ctcgactgag catggcgttg
     1081 gacataagga taaggaggag taaaccatga accgaacaag ttgggagcga atactgaagg
     1141 agtaatcact ctccatcgct gttggcacag aagtcattat catattcaag aatgcgcgag
     1201 atcacgtaag cggcgcgatc aaggacattg atgaggactt tcttattctg gaacacaagg
     1261 atggcaataa gatttcagcc attgtagaat tggatgaagt tgctggtttc agaacttgtt
     1321 gatcgtgcaa tactctgaga gatggttcca tgaatgagta tgagttttct gaacatgctt
     1381 acgacatgct gagggaacgg aacattcaag agttctgggt gaagttgagg
//
LOCUS       DAOQUC010001241         1428 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_126438_length_1428_cov_3.726875, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001241 DAOQUC010000000
VERSION     DAOQUC010001241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1428)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1428)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1428
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126438_length_1428_cov_3.726875"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1099)
                     /locus_tag="PKH07_17255"
     CDS             complement(<1..1099)
                     /locus_tag="PKH07_17255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36739.1"
                     /translation="MTQTPETISYFPKVVPADRLSTIRVAVLEPSPPFDSSDSYEVAA
                     YPMEETAGQLHAESKKAVKTWCADGSLFLSHEFISEQEHLLKIEKISACGRQPIGEIR
                     IYSLSKDLYALRPFKGDFHMHSSQSDGKEPPAHVAAACRRIGLDFMALTDHRKHAPSL
                     EAIAAFESVPLDIMMFPGEEVHSPGNRVHIINFGGRFGITPFFADEEAFRALVQQRGR
                     KLKNKLEPREKEQYAASVWCYDKIREAGGLAIFCHPYWVERERYNVSEPLIAQHFEDC
                     LFDAYEVIGGFHRHQIESNCLQVARYHEERAKGKKIPIVGVSDAHGCESGELFGWYYT
                     VLFAECLQLPDIVRSVKSLQSVAVEALPGEAP"
     gene            complement(1096..>1428)
                     /locus_tag="PKH07_17260"
     CDS             complement(1096..>1428)
                     /locus_tag="PKH07_17260"
                     /inference="COORDINATES: protein motif:HMM:NF016236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaA family lipoprotein"
                     /protein_id="HNT36740.1"
                     /translation="LWDPSTKLFKWPQYDEDFDQTLGVWGMGQGIFLTWPMIGPSSAR
                     ATLALPLDIISPYYAPFGLGMVFKINDASLGEADDYKTLKKMSIDPYLAFRDLYVQSR
                     LKKVHNRE"
BASE COUNT          325 a          381 c          381 g          341 t
ORIGIN      
        1 gaggcgcttc tccaggaagt gcttctactg ctacgctctg gagtgacttg acgctgcgga
       61 cgatatccgg cagttgcagg cattcggcga aaaggactgt gtaataccaa ccgaacaact
      121 cgccgctctc acagccgtgg gcgtcactga ctccgacaat gggaattttc ttgcctttgg
      181 ctcgttcttc gtgatagcga gccacttgca gacagttgga ttcgatctga tggcggtgaa
      241 atccaccaat cacctcatag gcgtcaaaga ggcagtcctc aaaatgctgt gcaatcagcg
      301 gttcggagac gttgtatctt tcgcgctcca cccagtatgg atgacaaaag atcgccagtc
      361 cacccgcttc ccgaatcttg tcgtagcacc agaccgaggc ggcgtactgc tccttctccc
      421 tgggctcgag tttgttcttc agttttctgc ctctctgctg caccaatgcg cggaaggcct
      481 cttcgtctgc aaaaaacggt gtgatgccaa aacgcccccc gaaattgatg atgtggactc
      541 tgttgcccgg agagtgaact tcctcgcccg ggaacatcat gatatccaag gggacgcttt
      601 cgaaagcagc aatggcttcc aacgagggag cgtgcttgcg gtgatcggtc agagccatga
      661 aatccagacc aatccgacga caggccgctg caacatgagc gggtggttcc tttccatcgg
      721 actggctgct gtgcatgtga aaatcccctt tgaagggtcg aagggcatag agatccttgc
      781 ttagagaata gatccggatt tccccaatcg gttgccttcc gcaagcagaa atcttctcga
      841 ttttcagaag atgctcctgt tcggaaatga actcgtggct gagaaagagg gaaccatctg
      901 cacaccacgt cttcacggct ttcttggatt ccgcgtggag ttggcctgcc gtttcttcca
      961 tgggataagc ggcgacctca taggaatcgg aggagtcgaa cggcggggat ggctccagta
     1021 ccgcaactcg aatcgttgac agcctgtccg cagggacaac cttggggaag tagcttattg
     1081 tctctggcgt ctgtgtcatt cgcgattgtg aacttttttc agtcggcttt gaacatagag
     1141 gtcccggaat gccagatacg ggtcaatgga catcttcttg agcgtcttgt agtcgtctgc
     1201 ttctcccaga gacgcatcat tgatcttgaa taccatgccc aaaccaaacg gggcataata
     1261 cggagagatg atatccaagg gcaacgccag cgttgcacga gctgaagacg ggccaatcat
     1321 gggccatgtc aagaagattc cctgtcccat gccccagact ccaagcgtct ggtcaaagtc
     1381 ctcgtcatat tgtggccatt tgaagagttt cgttgaagga tcccacag
//
LOCUS       DAOQUC010001242         1427 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_126578_length_1427_cov_3.521866, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001242 DAOQUC010000000
VERSION     DAOQUC010001242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1427)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1427)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1427
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126578_length_1427_cov_3.521866"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1427
                     /locus_tag="PKH07_17265"
     CDS             <1..>1427
                     /locus_tag="PKH07_17265"
                     /inference="COORDINATES: protein motif:HMM:NF013595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36741.1"
                     /translation="ALLFVRMRYLNTRKADEQTIPYAPSVSRTIAKDVGLLFAVLATI
                     IGVYSLLFTVFFTKAQGMTDGVHLYLSYWLKMHGKPRIPGPSTYYVARMILYEPLAML
                     GLVFALFYYPYRIGIRWFVRRDAELVEKYPYPSWFPIFFIIWSVFTLWIYSILEEKVQ
                     WLMYHQAMPCLVLAGYVVGELHERMRPGVMRKFLWTVVALLLAYQIRATTLCTHFEPD
                     DPRELLVYTQTNSDVTKVLKEIEGYSHILRGRGEKAIIQTQGETTWPFIWYLRDYETR
                     SGVVTGQDVPIILTDLEQKSRMKAVLEDKFIGRQYRLRAWWQPGQSWSDMSHSKDWFS
                     KLWRWFIYREPWDKDKYIGSTDFMFYVRRDLLPGATLPPETEMQDLTPINPTVSSSQP
                     AKLELLASWGGAGSGDGQMNLPRGLALSPDGNLYVADSRNHRIQAFDSSGKFLFQWGT
                     AGDGNSQFNEPSDVALASDGSVWVT"
BASE COUNT          324 a          375 c          373 g          355 t
ORIGIN      
        1 atgcgctgtt gtttgtgaga atgcgatatc tgaacaccag aaaggcagac gaacagacta
       61 tcccgtacgc tccaagcgtt tctcgaacga tcgccaaaga cgtcggactt ctcttcgccg
      121 tactggcgac aatcattggt gtctacagtc ttctcttcac agtgttcttc accaaggccc
      181 aaggcatgac ggatggagtc cacttgtatc tgtcctactg gctgaagatg catggcaaac
      241 caaggattcc agggccaagc acgtactatg ttgccaggat gattctctat gagcctttgg
      301 caatgttggg tctggtattc gcattgtttt actatccgta tcgtattgga atccggtggt
      361 ttgttcgtcg tgatgccgag ttggttgaga agtatcccta tcccagctgg tttccgatct
      421 tctttatcat ctggtcggtg ttcactctct ggatttattc cattctcgaa gaaaaggttc
      481 aatggttgat gtaccatcag gcaatgccct gcctggtgct ggcaggatac gtcgtcgggg
      541 aacttcatga gcgcatgaga cctggagtca tgcgcaaatt cctctggacg gtagtagcgc
      601 ttctattggc gtatcaaatc cgtgcaacca cgctgtgtac ccactttgag ccggacgatc
      661 cgcgcgaact tctggtctat acccagacca acagcgacgt gacaaaagtg ctcaaggaga
      721 tcgagggata cagccacatt ctcagaggtc gtggagaaaa ggcgatcatc cagactcagg
      781 gcgagaccac atggccattc atctggtacc tgcgggacta cgaaacgcga tcaggtgtgg
      841 ttaccggtca ggatgttccg attatcctga ccgacctgga acagaaatct cgcatgaagg
      901 cagttcttga agacaagttt atcggtcgtc agtatcgtct gcgcgcctgg tggcagccag
      961 gtcagtcctg gtctgacatg tctcattcga aagactggtt ttcgaaactg tggcgttggt
     1021 tcatctatcg agaaccatgg gataaagaca agtacatcgg ctccaccgac ttcatgttct
     1081 acgtgcggcg cgacctcctg ccaggagcca ctctcccacc agagaccgag atgcaggatt
     1141 tgacaccgat caacccaaca gtctcctcct cgcaacctgc aaagttggag ttgcttgcct
     1201 cctggggcgg cgcaggcagt ggcgacggac agatgaacct gcctcgggga ctggcactct
     1261 ctcccgacgg caatctctat gtagccgact ccagaaatca ccgcattcaa gcattcgact
     1321 catccggcaa gttcttgttc cagtggggaa ctgccggtga cggaaacagt cagttcaatg
     1381 aaccttctga tgtcgccttg gcttcagatg gttcagtctg ggtgacc
//
LOCUS       DAOQUC010001243         1425 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_126920_length_1425_cov_2.416058, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001243 DAOQUC010000000
VERSION     DAOQUC010001243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1425
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126920_length_1425_cov_2.416058"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..474
                     /locus_tag="PKH07_17270"
     CDS             <1..474
                     /locus_tag="PKH07_17270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36742.1"
                     /translation="GVFDPDFIQTFQGSEERPYFMPTQGLVMEAITECFVQWWKGVLE
                     FFPNILPDWISSSENEPIIFRSFRTPGAFLISGVLQGNQEYVKIECEKGGQLRFRIPA
                     RWRQGTLFDTSRSYLCTVPGEQIITTDTLPGEVFHLYRTSPCTHSTGFPNDIANN"
     gene            complement(464..1042)
                     /locus_tag="PKH07_17275"
     CDS             complement(464..1042)
                     /locus_tag="PKH07_17275"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNT36743.1"
                     /translation="MWPNTQRAFTLIELLIVVAIIGILAAIAVPNLLQAQTRSKVARV
                     KADHSAYCTAVESYMIDHNTYPLDGDDVPFFDPTLWYQKAQAARLTTPVAYIKTILDD
                     PFNTRPATSPGMFLLFPDSPPQPYAYMTLGGYPIHAGRPNRYGVVSVGPNGEFDSAAN
                     QGIDDTYDPTNGVVSRGDIIRYGPGGPGRFSY"
     assembly_gap    1172..1181
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1182..>1425)
                     /locus_tag="PKH07_17280"
     CDS             complement(<1182..>1425)
                     /locus_tag="PKH07_17280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HNT36744.1"
                     /translation="LYEGHLYGFDEADLKCIEWSTGTEKWSQRGLGKASLMIANDKLI
                     IMGERGDAVVAEATSDAYKEVSRSSVLEGRCWVVPVL"
BASE COUNT          348 a          365 c          349 g          353 t
ORIGIN      
        1 ggtgtctttg atcctgactt cattcagaca tttcaaggct cagaggaaag gccttacttc
       61 atgccgacac aaggccttgt gatggaagca atcacggaat gttttgttca gtggtggaaa
      121 ggcgttctgg agttctttcc caacattctg ccggattgga tctcctcttc agaaaatgag
      181 cctattattt tccggagttt ccgcacacca ggagcattct tgatttcagg cgtcttgcag
      241 ggaaaccagg aatacgtgaa gatcgagtgt gagaagggcg gtcaactgcg ttttagaatc
      301 cctgcaagat ggcggcaagg taccctcttc gatacaagtc gcagttatct gtgcaccgtg
      361 cctggcgaac agatcatcac cacggacacc ttaccaggtg aggtattcca tctctatcgc
      421 acctcaccgt gcactcatag tacgggtttc ccaaacgaca tcgctaataa ctgaatcgtc
      481 caggcccccc aggaccgtat cggataatat cgccacgact caccacccca ttggtcggat
      541 cataggtgtc atcaatgcct tggtttgcag cagagtcaaa ctccccattg ggaccgaccg
      601 aaactactcc atatctgttg gggcgtcctg catgaatcgg atatcctccc agagtcatgt
      661 aagcgtaggg ctgagggggc gagtcgggaa acaggaggaa cattccagga ctggtcgccg
      721 gacgcgtgtt gaatggatca tcaagaatgg tcttgatata ggcgacaggt gtagtgagcc
      781 ttgctgcttg agctttttga taccacagag ttgggtcaaa gaacgggaca tcgtctccgt
      841 cgagtggata ggtattgtga tcgatcatgt atgattcgac agcggtgcag tatgctgagt
      901 ggtctgcttt gacacgcgca accttggatc gagtctgcgc ctgaagaaga ttcggtacgg
      961 caatagccgc aagaatgccg atgatcgcca cgacgatcaa cagttcaatt agcgtaaatg
     1021 ctctttgagt gttcggccac atctgtctgt cactccttcg atacacagcc tggagaagac
     1081 ttccagatcc atatctcact ttctcaacta agactagtct ttccgcaaat caagacagac
     1141 caaatcccca ttgttgtttt tgcagtacaa annnnnnnnn naggactgga accacccagc
     1201 agcgaccttc cagaacgctg gagcgggaca cttccttgta ggcatccgaa gtggcctcag
     1261 cgacaacagc gtcgcctcgc tctcccatga tgataagttt gtcattggct atcatcaatg
     1321 aggctttgcc caagccacgc tgcgaccatt tctctgtgcc tgtgctccat tcaatgcact
     1381 tcagatcggc ttcatcaaat ccgtacagat gtccttcata gagaa
//
LOCUS       DAOQUC010001244         1423 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_127206_length_1423_cov_2.548246, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001244 DAOQUC010000000
VERSION     DAOQUC010001244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1423
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_127206_length_1423_cov_2.548246"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..883)
                     /locus_tag="PKH07_17285"
     CDS             complement(<1..883)
                     /locus_tag="PKH07_17285"
                     /inference="COORDINATES: protein motif:HMM:NF024120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HNT36745.1"
                     /translation="MNRDTRDTQGTPPEINRRQVLALSGMAAAGIPVSLAAIAGCVGN
                     PVCRQMTDINKNGEGVQMLVNVRDFGAVGDGVTDDHKAIQRSFQEGLTESGGIVFFPP
                     GRYRCGGRIVSEAKPVSVLGAGRGSSVICFTATEEDQVGFLFDQQVYTNTVELRSLTL
                     TTDQAEPGDALTVRYSSQDCRAVRIYERVYLENINIVGTDTACHGFRNGVCLEHVNSP
                     LLLNVSVSGRQPAAGLSNRTHTNACFKLIGGALQTGGIPVQSGFYRCSGYNARFGIHV
                     SGAHEGMIVHGGNFVECG"
     gene            complement(885..>1423)
                     /locus_tag="PKH07_17290"
     CDS             complement(885..>1423)
                     /locus_tag="PKH07_17290"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNT36746.1"
                     /translation="SEVYRLRDMVHEFFIREFELWSTTDVDAVMFMDDWGTQQALLIS
                     PEMWREFFKPLYRDYVSIAHSKKKFVFMHSDGMIEAIYPDLIEIGVDALNSQLFCMDI
                     ERLGREFGGKITFWGEIDRQRLLPFAAADEVSAGVRRVVTALGNFNGGLIAQCEWGSD
                     VRSVNVRAVFETFCEMGD"
BASE COUNT          365 a          447 c          312 g          299 t
ORIGIN      
        1 caccgcactc aacaaaattg cccccatgaa caatcatccc ttcatgcgcc ccggaaacat
       61 gaatgccaaa acgggcattg tagccagagc acctgtagaa ccccgactga acgggaatcc
      121 cgcctgtctg aagtgcgccg ccaatgagtt tgaagcaggc gttcgtatga gtccgattac
      181 tcagtcccgc tgctggctgt cggccggata cggagacatt caacagaagg ggtgagttca
      241 catgctccaa gcatactccg ttgcggaaac cgtgacacgc agtatccgtt cccacaatgt
      301 tgatgttctc aagatagacc cgttcgtaga tccgcaccgc ccgacagtcc tgagacgaat
      361 accgaacggt caaagcatcc cccggttcgg cctgatcggt ggtgagcgtc agactccgca
      421 gctccactgt gttggtataa acctgctgat caaaaaggaa accaacctga tcctcctccg
      481 ttgcggtaaa acagatcacg ctgcttcccc gtcccgctcc cagcaccgaa acaggtttcg
      541 cctctgagac aatccgccct ccacaccgat accgacctgg gggaaagaac acgattcccc
      601 cagactcagt cagaccttcc tgaaacgatc tctgaatcgc cttatgatca tcggtaaccc
      661 catctccaac ggcaccgaag tctcgcacgt tcacaagcat ctgtacgccc tctccattct
      721 tgttgatatc agtcatctga cgacagacag gatttcccac acaacctgcg atggcagcaa
      781 gactgactgg aatgcctgca gcagccatgc cactcaaggc aagaacctgc ctgcgattga
      841 tctccggagg ggttccttgg gtgtctctgg tatcccggtt catctcaatc acccatctca
      901 cagaaagtct caaaaaccgc cctcacgttg acggagcgca catccgatcc ccattcacat
      961 tgagcgatca agccaccatt gaagttgccc agtgcagtta caacacgtcg gacgcctgcg
     1021 ctgacttcat ccgccgcagc gaagggcaag agtctctgac gatcgatctc tccccagaac
     1081 gtaatcttac caccaaactc ccttcccagt cgctcgatgt ccatgcagaa aagttgtgaa
     1141 ttcagtgcat ccacaccaat ctcgatcagg tctggataga tggcttcaat catcccgtcg
     1201 ctgtgcatga acacaaactt tttcttcgaa tgggctatcg aaacatagtc ccgatagagt
     1261 ggcttgaaga actcacgcca catctctgga gagatcaaca gtgcctgctg tgttccccag
     1321 tcatccatga acatcactgc gtcaacatcc gtcgtagacc agagttcaaa ctcacggatg
     1381 aagaactcat gaaccatgtc acgcaaccgg tacacttcgg aga
//
LOCUS       DAOQUC010001245         1421 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_127444_length_1421_cov_3.217423, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001245 DAOQUC010000000
VERSION     DAOQUC010001245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1421)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1421)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1421
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_127444_length_1421_cov_3.217423"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..268
                     /locus_tag="PKH07_17295"
     CDS             <1..268
                     /locus_tag="PKH07_17295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36747.1"
                     /translation="TPEKQDQSWDEHVPIARIMESPTFVKNASDAVEQSDCHICGNRY
                     RIPESMRMPLAQWLQGSVLFGFAGLGAAEQVTSHISSSCHRNWS"
     gene            271..>1421
                     /locus_tag="PKH07_17300"
     CDS             271..>1421
                     /locus_tag="PKH07_17300"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HNT36748.1"
                     /translation="MNRTMSSWIRVLLSLMTGALVLSDAAVFAQEPVAVPALTAAPTV
                     DGIMEPEYRQGAQLVDFWDFAKSVVEARPASKDTRVWLGHFNRTLYVFCEASEPQTNL
                     LKTTVTLHDGSAWTDDSFELFLDIEGQRKNYFQFILSSNGTTADFFKGQKSWNAEYDV
                     AAKIWSSSWSAEWAIPFKAFGIASVDDINAEKRWTFNICRNDIVGDELLTWVPVNSAA
                     VRNFGTPALFAEMHLVLEGGAKTYSLRGTPLRPFEMVREQLTWKTTDPLFGELLSKKH
                     RLTPGHSGFFWTHPVGASQLRPMAVQYGVEYSLEETGDLFKSLGMHPIQVGQTIQKWV
                     ENADVPDRYPTAGAMYATNFKYARAEGMPTVESSGLTILFAPRNRERYL"
BASE COUNT          337 a          349 c          377 g          358 t
ORIGIN      
        1 cactcccgaa aaacaggatc agtcttggga tgaacacgtc ccaattgcaa ggatcatgga
       61 aagcccaaca tttgtcaaga atgcttcgga tgccgttgag caatcggatt gtcatatctg
      121 tgggaatcgg tataggattc ctgaatcaat gaggatgcct ttggcgcagt ggctccaagg
      181 atcggtcttg tttggatttg cgggtttggg tgcagcggaa caagtgacga gccacatttc
      241 ctcatcttgt cacagaaact ggagttgaac atgaatcgaa caatgagttc ctggatccgt
      301 gtcctgcttt ctttgatgac aggtgccttg gtactgtcgg acgctgcggt ctttgcacag
      361 gagccagttg ctgttcctgc gttgactgct gccccaacgg ttgacggtat catggagcct
      421 gaataccgac agggcgctca actggtggat ttctgggatt ttgccaagag cgttgtcgag
      481 gcacgtcccg catcgaaaga tacccgtgtt tggcttgggc acttcaacag gacactctac
      541 gtcttctgtg aagcttctga acctcagaca aacctgctca aaactacggt gacactccac
      601 gatggcagtg cctggacgga tgattccttc gaactgtttc ttgacatcga aggtcagcgg
      661 aagaattact ttcagttcat cctttcctcc aacggaacca cggcggactt tttcaaaggg
      721 cagaagtcct ggaacgctga atacgacgtc gcggcgaaga tatggagcag cagttggagt
      781 gctgaatggg cgattccttt caaggctttt ggaattgcct cggtggatga catcaatgcg
      841 gagaaaagat ggacattcaa catctgtcgt aacgatatcg ttggagacga actgctcacc
      901 tgggttcctg tcaattccgc ggctgtgaga aacttcggca ctccggcgct ttttgcagag
      961 atgcatctcg ttctcgaagg cggcgcaaag acctattcat tgcgggggac acctcttcgt
     1021 ccatttgaga tggttcgaga acaactgacg tggaagacca cggatccact atttggcgaa
     1081 cttctgtcca aaaagcacag attgacccca ggtcattcag gattcttctg gacgcatcct
     1141 gtgggtgctt cccaacttcg tcccatggca gttcagtatg gggtcgaata ctctcttgag
     1201 gaaacaggcg atctgttcaa atcccttgga atgcacccta ttcaagttgg tcagaccatt
     1261 cagaaatggg ttgaaaatgc cgatgtgcct gacaggtatc ctaccgctgg cgcaatgtac
     1321 gcgaccaatt tcaaatacgc gcgtgcagag ggtatgccca ccgtcgagag ttccggtctc
     1381 acgatcctct ttgcacccag gaatcgagag cgatatctgc a
//
LOCUS       DAOQUC010001246         1417 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_128121_length_1417_cov_2.581498, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001246 DAOQUC010000000
VERSION     DAOQUC010001246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128121_length_1417_cov_2.581498"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..454)
                     /locus_tag="PKH07_17305"
     CDS             complement(<1..454)
                     /locus_tag="PKH07_17305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014808677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I CRISPR-associated protein Cas7"
                     /protein_id="HNT36749.1"
                     /translation="MSELIKNRYEFMFLFDCENGNPNGDPDAGNAPRLDPEDMHGLVS
                     DVALKRRIRNYVQVAKDNQMPYSIFVEHATNLNRPITKAHEQTEGGLDTSGKGANKKK
                     VEMARQWMCDNFYDVRAFGAVMSIGPNAGQVRGPVQLAFARSVDPILAM"
     gene            complement(447..>1417)
                     /locus_tag="PKH07_17310"
     CDS             complement(447..>1417)
                     /locus_tag="PKH07_17310"
                     /inference="COORDINATES: protein motif:HMM:NF021243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas8c/Csd1"
                     /protein_id="HNT36750.1"
                     /translation="RLAGAKVIHWFDKKVPIEDDPFYFLAEPPEQEELNAQIVARQLL
                     ESIRSGKRVDLQRNHYYSMTLCGASGRVMVRDWMEGQFEELVQSVSAWFDDLAIVRRD
                     GTSLAPAPKFVAVLGATVRDLKDLNAPFVAKMWRVALRAEPIPSSAMAQALNRVKIDI
                     IQDSPFSHARMGLLKAYHIRKISNSGGLSMTEEMKPYLNEQHPSVAYQCGRLMAVLAA
                     LQRSALGDVGAGVVQRYYAAASTTPALVFGRLVRTSQFHLNKLEPGLAHWYEDRLANI
                     WSRIRDSVPKTLTLEQQSLFALGYYQQLAAPKGKQNNIEKGATTNE"
BASE COUNT          335 a          395 c          350 g          337 t
ORIGIN      
        1 ccatggcaag aatcggatcc accgaacgtg caaaggcaag ctgaacaggg ccacggacct
       61 gacccgcatt tggtccaatg ctcatgacag ctccgaaagc acgaacatcg tagaagttgt
      121 cacacatcca ttgtcgcgcc atctcgacct tcttcttgtt tgcacccttg cccgaagtgt
      181 ccaagccacc ttcggtctgc tcatgcgctt tggtgattgg tctgttgagg ttggttgcat
      241 gttcgacaaa gatcgaatag ggcatctgat tgtccttggc aacctgaaca tagttacgaa
      301 tccgacgctt caaagcgacg tcagacacca agccatgcat atcctcagga tccagcctcg
      361 gggcattgcc tgcgtcagga tctccattgg gattgccatt ctcacagtca aacagaaaca
      421 tgaattcgta tcggttctta atcagttcac tcattggtcg ttgctccttt ttcgatattg
      481 ttctgcttcc ccttgggggc ggctaattgc tgatagtatc caagtgcaaa gaggctctgc
      541 tgttccaagg tcaaagtctt tggaacgctg tcacgaatcc gagaccagat gttggccagc
      601 ctgtcctcat accaatgcgc gagacctggt tccagtttgt tgagatggaa ctggctggtg
      661 cgcacgagcc ttccaaaaac cagtgccggc gtcgtggaag cagcagcgta atacctctga
      721 accacgccag ctcctacatc acccagggca gaccgctgca aggcggccag aacagccatc
      781 agtcgtccgc attgatatgc aacgctcgga tgctgttcgt tcaagtacgg tttcatttct
      841 tcagtcattg ataaacctcc tgaattcgat atcttgcgaa tgtgataggc cttcaatagt
      901 cccatgcgag catggctgaa cggagagtct tggatgatgt caattttgac acggttgagc
      961 gcttgagcca tcgctgatga gggaattggc tccgcacgaa gagcgacacg ccacatcttc
     1021 gctacgaaag gtgcattcaa atccttcagg tcacgaacgg tcgcacccaa caccgcgacg
     1081 aacttcggag caggagccag ggaagtgcca tcccttctca caatggcaag gtcgtcaaac
     1141 catgcggaga cactctgaac cagttcttca aactgacctt ccatccaatc gcgcaccatg
     1201 actcttccac tggcaccgca gagggtcatt gagtagtagt gattgcgttg caggtcaaca
     1261 cgcttcccag agcgaatgct ctcgagcaac tgcctggcga caatctgagc gttcagttct
     1321 tcctgctccg gtggctcagc aagaaagtag aacgggtcgt cctcaatggg caccttctta
     1381 tcaaaccagt ggatgacttt ggcccccgca aggcgtt
//
LOCUS       DAOQUC010001247         1409 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_129290_length_1409_cov_3.645495, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001247 DAOQUC010000000
VERSION     DAOQUC010001247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1409)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1409)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1409
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_129290_length_1409_cov_3.645495"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1409
                     /locus_tag="PKH07_17315"
     CDS             <1..>1409
                     /locus_tag="PKH07_17315"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNT36751.1"
                     /translation="VRAADAVKKELTEIEGQLPSDMKIGISMDFSPMIKESIQTVKDN
                     AYQGAFLAAVVLLLFLRSWRSMLIILIAIPTSVVSTFFCMYLADMTLNLISLGGLALG
                     VGMVIDNSIVVIENIFRHRKNNPDQHPHDVAGNAASELALALTASTFTTVAVFFPIVF
                     VEGIAGEVFKDLALVIVFSILASLLVALTVVPCLGARLIARGDKPKSTVRAYVALCNS
                     FDRLMAWAPASFSAYGSGIKSVSRSTATRILTVLAAGVFSVAVFKYMQPSQAQFPKMD
                     QGMINVELEMPQGSNLKHTDQAALRLEKICMEVPEAQIVSSTVRGNKASVFISLGNVE
                     DRERSLWDVMDELRALSKDFAEGEVRVQEMSHGGGGEDKPVQIEIIGSNYTQIQNFAR
                     QIKARLQNVPGVWDVETSFEKGKPEIQIIIDRKRAADLGLSARTIAETIETYMTGTVA
                     GQFRVEGEEFDIRVRLQKE"
BASE COUNT          300 a          346 c          412 g          351 t
ORIGIN      
        1 ggttcgagcg gcggatgcgg tcaagaagga attgacggag atagaggggc agctgccctc
       61 tgacatgaag atcgggatct ccatggattt ctcgccgatg atcaaagagt ccattcagac
      121 tgtgaaggac aacgcctatc agggagcctt tctggccgct gttgtgctgc tcttgtttct
      181 gcggagttgg cgtagcatgt tgattatcct catcgcgatt cccacttccg tggtcagcac
      241 cttcttctgc atgtatctgg cggacatgac cctgaatctg atctcgctgg gtggtttggc
      301 tctgggagtg ggtatggtca ttgacaactc catcgttgtc atcgagaaca ttttccgcca
      361 tcggaagaac aatcccgatc aacaccccca tgatgtggct ggcaatgccg ccagcgaact
      421 cgcgcttgct ctcactgcgt ctacgtttac caccgttgcg gtgtttttcc cgatcgtgtt
      481 tgtggaggga attgccgggg aggtattcaa ggatcttgcc ctggtcatcg tgttctcgat
      541 tttggcgtca cttctcgttg cgcttaccgt tgtcccttgt cttggcgcaa ggctgattgc
      601 gcgaggtgat aagccgaaaa gcacggtgcg agcatatgtt gccctgtgca actcatttga
      661 cagacttatg gcatgggcac ctgcgtcgtt cagtgcctat ggttctggaa tcaaatccgt
      721 ctccagaagt actgcaactc ggattctcac cgtgttggcg gcgggagtgt tttccgtcgc
      781 ggtcttcaaa tacatgcaac cgagtcaggc gcagtttccc aaaatggatc aagggatgat
      841 caacgttgag ctggagatgc cccaggggtc caatttgaaa cacactgacc aggcggcgct
      901 tcgtttagaa aagatatgta tggaagtgcc ggaagctcag attgtctcct ccactgtacg
      961 cggcaacaag gcttccgtct tcatctcgct ggggaacgtg gaagaccgtg agcgctcgct
     1021 ctgggatgtc atggacgagc tgcgtgcgct ttcgaaggat tttgctgaag gggaagttcg
     1081 ggttcaggag atgagccatg ggggtggggg tgaagacaag ccagttcaga ttgagatcat
     1141 tggcagcaac tacacgcaga ttcagaactt cgccagacag atcaaagccc gactgcaaaa
     1201 tgttccggga gtctgggatg tggagacttc gtttgagaag ggcaaaccgg aaatccagat
     1261 catcattgat cggaaacgcg ccgccgatct tgggttgtct gcccgaacca tcgcagagac
     1321 catcgaaacc tatatgactg gcactgttgc gggtcagttc cgtgttgagg gcgaggagtt
     1381 cgacatccgt gtccgccttc agaaggagc
//
LOCUS       DAOQUC010001248         1408 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_129443_length_1408_cov_3.438285, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001248 DAOQUC010000000
VERSION     DAOQUC010001248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1408)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1408)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1408
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_129443_length_1408_cov_3.438285"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            42..>1408
                     /locus_tag="PKH07_17320"
     CDS             42..>1408
                     /locus_tag="PKH07_17320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006965544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNT36752.1"
                     /translation="MPESDLISAVKREQEGILIVGLAALFFAMVLAWFLSRRFSDPIA
                     QLTRAADQMASGDLHVQLPTAGSREIQSLSHSFDRMRSELQKQMNALREKSTELEIFF
                     SSALDLLCIADTQGNLIRVNKEWEKTFGFSVEQLEGMKFLDLVHPDDLQETLEAVAKL
                     ERQEQVLNFTNRYRCRDGSYRLIEWRSSPRGSLIYAAARDITERRRAEEELQLSRERL
                     DLGMDASNSGLWDWNVADNTVYFDPRYFTMAGYQPDEFPHAFAEWEKRVHPDDIDYCR
                     SEIDAYLSGRSAAFYAEFRFLRNDGSWMWIRGHGKAVERGENGAVRRMVGTHADITER
                     KQAEFERQKLQEQLAQAQKMESVGRLAGGVAHDFNNMLTVINGHTAMALEKAAGSHAI
                     YQDLLEIRRAAQRSADLTRQLLGFARRQVIAPSNLDLNKTVEGMLSMLRRLIGEDIHL
                     DWRPG"
BASE COUNT          317 a          353 c          428 g          310 t
ORIGIN      
        1 ttcgggtttc tccgcagcgg acactctggg taggtgttct ggtgccagag tccgacctta
       61 tcagcgctgt gaaacgcgag caggaaggca ttctgattgt agggctggcc gccctgttct
      121 ttgcaatggt gctggcatgg tttctctctc gccgattttc tgatcctatt gcacaattga
      181 cccgagccgc ggatcagatg gcgtcgggag atctgcacgt ccagctgccg actgccggtt
      241 ccagagagat tcagtctctt tctcattcct ttgatcggat gagaagtgag ttgcagaaac
      301 aaatgaacgc tttacgggaa aagtccaccg agttggagat cttcttctca tcagccctgg
      361 atctgttgtg cattgccgac acgcagggaa acttgatccg cgtcaacaaa gagtgggaga
      421 agacgtttgg tttcagtgtc gaacagcttg aggggatgaa attcctggat ctcgtccacc
      481 cggacgatct ccaggagaca ctggaggcgg tcgccaagct ggaacgtcag gaacaagtcc
      541 tgaatttcac caaccgctat cggtgtcgcg acggttctta tcggttgatc gaatggcgtt
      601 cgtcgccacg gggctctctg atctacgcgg cagctcggga catcaccgag cggcggcgtg
      661 ccgaagagga actacagctg agtcgtgagc ggctggatct cggaatggat gcctcaaact
      721 cagggctttg ggactggaac gttgccgaca acacagtgta tttcgatcca cgctatttca
      781 caatggcggg ctaccaaccg gatgaattcc cacacgcttt cgcagagtgg gaaaagcgag
      841 tgcatcctga tgacatagac tattgtcgct cggagatcga cgcgtatctt tcgggtcggt
      901 cggcagcgtt ctatgcggag ttccggtttc tccgcaatga tggctcgtgg atgtggattc
      961 gaggtcacgg gaaagccgtc gagcgaggtg aaaacggagc ggtgcgacgg atggtgggaa
     1021 cccatgctga catcaccgag cgcaaacagg ccgaattcga aagacagaaa ctgcaggagc
     1081 agttagcaca agcgcagaag atggaatccg tcggacggct tgccgggggt gtggcacacg
     1141 atttcaacaa tatgctcacc gtcatcaatg ggcatacggc gatggcattg gaaaaggcgg
     1201 ctggctctca cgcgatctac caggatctgt tggagatccg aagagcagca cagcggtctg
     1261 ccgacttgac acgacagctt ctgggttttg cgcgcagaca ggtgatcgcc ccaagcaacc
     1321 tcgatttgaa taagacggtc gaggggatgc tgagcatgtt gcgacggctc attggtgagg
     1381 acatccatct tgactggaga cccggcaa
//
LOCUS       DAOQUC010001249         1406 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_129795_length_1406_cov_2.818653, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001249 DAOQUC010000000
VERSION     DAOQUC010001249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1406)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1406)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1406
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_129795_length_1406_cov_2.818653"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1406)
                     /locus_tag="PKH07_17325"
     CDS             complement(<1..>1406)
                     /locus_tag="PKH07_17325"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNT36753.1"
                     /translation="MAVQAELASIAKSEFLANMSHEIRTPMNGIIGMTGLLLDTELTD
                     EQRRFAETVRTCGESLLTLINDILDFSKIEAGKLDLEILDFDLQNLLDDFAFTFALRA
                     HEKGLELLCSADPDVPSLLRGDPGRLRQILTNLVGNAIKFTHAGEVAVRVSLESETES
                     ETKLRFSVSDTGIGIPRDKLDIIFNKFAQVDASTTRQYGGTGLGLAISKQLAEMMGGE
                     IGVQSHLGQGSEFWFTARFVKQPGDRRRKSPKADLHGVKILVVDDNATSRSILSMRLS
                     SWGMRVSLAVDGTEALALLREAADGKDLFEVVVTDMHMPGMDGESLGLAIKADSRLSS
                     IRLIMLTSLGSRGDARRFEEIGFSGYLPKPMRHQELWAVLVTVLGSTVTSESAHDEGV
                     AVESKGIVTRHSAKECMDSAAGHGTGDALDFEASGARILVVEDNTVNQQVALGIIKKL
                     GLRAEAVANGEEALKTLE"
BASE COUNT          333 a          456 c          308 g          309 t
ORIGIN      
        1 ctcaagcgtc ttgagtgctt cctcgccatt ggcaaccgct tccgctcgca gacccagctt
       61 cttgatgatc cccaaagcca cctgctggtt gaccgtattg tcctcgacaa ccagaatcct
      121 cgccccgctg gcctcgaaat ccaacgcatc ccctgtccca tgtcctgcag ccgaatccat
      181 gcattctttc gcagagtgcc gagtgacaat accctttgac tccaccgcca cgccttcgtc
      241 gtgcgcactt tcagatgtga cagtggaacc caagactgtg accagcacag cccacaactc
      301 ctgatgcctc atcggcttgg gcagatagcc cgaaaagcca atctcctcaa atctccgagc
      361 atcacctcgt gacccaagag aggtcaacat gatcaaacga atacttgaga gcctcgaatc
      421 cgctttgatc gcgagaccca gcgattcccc atccatgcca ggcatgtgca tatctgtgac
      481 aaccacttca aaaagatcct tcccatccgc ggcctcacgc aacagagcca gggcttccgt
      541 tccgtccaca gccagactga ctctcatccc ccacgaactc aggcgcatac tgagaatgct
      601 gcgactggtg gcattgtcat ccacaaccag gattttgacg ccatgcagat cagcctttgg
      661 cgatttgcgc ctgcgatctc ccggttgttt cacaaaacgc gcggtgaacc agaactcgga
      721 accttgaccg agatggcttt gaacaccaat ctccccaccc atcatctcag ccagttgctt
      781 ggaaatcgcc aagcccaacc ctgttccgcc atactgcctg gtcgtggacg catccacctg
      841 cgcaaacttg ttaaagatga tgtccagttt gtctctcggg ataccaattc cggtatcgga
      901 aaccgagaac ctgagctttg tttcgctctc agtctccgac tcaaggctga ctcgcacagc
      961 aacttcacca gcatgagtga acttgatggc attgccgaca aggttggtca gaatctggcg
     1021 cagtcgtcca ggatcccccc gcaacaggga cggcacatca ggatcggcac tgcacaacag
     1081 ttccaagccc ttctcatgcg cccgcaatgc gaaggtaaac gcgaaatcat cgagcaggtt
     1141 ctgcagatca aagtccagaa tctccagatc cagttttcct gcctcgattt tcgagaagtc
     1201 gagaatgtca ttgatcagtg tcagcagcga ctcgccacac gtccgaactg tttctgcgaa
     1261 acgacgctgc tcatcagtca actccgtatc cagaagcaag ccggtcatac cgataattcc
     1321 attcatcggc gtgcgaattt catggctcat gttggcaagg aattcactct tggcaatgct
     1381 cgccagttcc gcctgcacag ccatct
//
LOCUS       DAOQUC010001250         1404 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_130072_length_1404_cov_3.817643, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001250 DAOQUC010000000
VERSION     DAOQUC010001250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1404
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130072_length_1404_cov_3.817643"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1404
                     /locus_tag="PKH07_17330"
     CDS             <1..>1404
                     /locus_tag="PKH07_17330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36754.1"
                     /translation="LLPEAFAVCREMARRIGQPDRWNSYNVPYNRLLPHFPVQLMGGM
                     ALFEGCIAEMATGEGKTIVATLPAYLIGITGEGCHIVTVNDYLARRDSEWMGPLYREL
                     GLTVGVIQMDMPPEDRRVAYGSDITYGTNNEFGFDYLRDNMAWTPEHCVQREHNYAIV
                     DEVDSILIDEARTPLIISGPAEESTDKYYVINSIIPKLSRGEHYTVDEKEHTVMLTEA
                     GIDRAQSLLGLENMFTEENLDLIHHINQAVRAHTLYQRDTQYIVQDGKVIIVDEFTGR
                     LMPGRRWSDGLHQAVEAKEGLRIERENQTLATITFQNYFRLYDRLAGMTGTADTEAAE
                     FHEIYGLPVRVIPTDKPLRRVNNPDVVYKTQREKFEAIIEEIVDCLERGQPVLVGTIS
                     IEVSELVSKMLKEGTYWNEQLRLASSAIQETLRESQETEVLQQVRTALKKLHEMQEAA
                     LDEVESLLNGSKKAGTIG"
BASE COUNT          353 a          355 c          401 g          295 t
ORIGIN      
        1 cctgcttcca gaagcctttg cggtctgtcg cgagatggca cgccgcatcg gacagcccga
       61 tcggtggaat tcatacaatg ttccgtacaa tcggctgttg ccacactttc ctgttcagct
      121 gatgggtgga atggcgcttt ttgagggctg cattgcggaa atggcgacag gcgaaggcaa
      181 gacgatcgtg gcgacactgc ccgcgtatct catcggaatc accggcgagg gctgccacat
      241 tgtgacggtc aacgactatc tggcgcgtcg tgacagtgaa tggatggggc ctctctatcg
      301 ggaactcggg ctgacagttg gcgtcattca gatggatatg ccgccggaag accgccgagt
      361 tgcctacggt tcggacatca cgtacggaac caacaacgaa tttggttttg actatcttcg
      421 cgacaatatg gcgtggactc ctgaacactg cgtgcaacgt gagcacaact acgccatcgt
      481 ggatgaagtg gacagcatac tcattgacga ggcacgaacg cctctgatca tctcagggcc
      541 tgcagaggaa tcaacggaca agtattacgt aatcaacagc atcatcccca aactcagtcg
      601 cggggaacac tacacagtgg atgagaaaga acacacagtg atgctgacag aggcaggaat
      661 cgatcgcgct cagagccttc tggggctgga gaacatgttt acggaagaga atcttgacct
      721 catccaccac atcaaccagg cagttcgagc gcatacgctt taccaaaggg acacgcaata
      781 tattgtccag gatggcaaag tgatcatcgt tgatgagttt accggtcgtc tgatgcccgg
      841 ccgacgctgg agcgatggac tccatcaggc tgtcgaagcc aaagagggtc tgcgaatcga
      901 gcgagagaat caaacgctgg cgacgatcac ctttcagaac tattttcgtc tctacgaccg
      961 actggcaggt atgacgggaa ccgcagacac agaggccgct gagtttcatg agatctacgg
     1021 tcttccagtt cgtgtcattc ccactgataa gcccctgcgg cgcgtgaaca atccggatgt
     1081 tgtctataag acccaacggg agaaattcga agcgatcatt gaggagattg ttgattgtct
     1141 cgaacgggga caacccgtgt tggtcggaac catctccatt gaggtatccg aactggtttc
     1201 gaagatgctc aaggaaggca cttactggaa cgagcagctg cgacttgcct cgtcagccat
     1261 tcaggaaacg ttacgcgagt cccaggagac cgaggtcttg cagcaggttc gaaccgcgtt
     1321 gaagaaactt catgagatgc aggaagccgc gctagatgag gttgagagtc tcttgaatgg
     1381 ttccaagaag gcggggacca tcgg
//
LOCUS       DAOQUC010001251         1404 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_130101_length_1404_cov_3.040771, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001251 DAOQUC010000000
VERSION     DAOQUC010001251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1404
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130101_length_1404_cov_3.040771"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1404)
                     /locus_tag="PKH07_17335"
     CDS             complement(<1..>1404)
                     /locus_tag="PKH07_17335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36755.1"
                     /translation="TINLAHANSVRDVHPGKEEDLRDLVRLGGHNTYSCPGSGEVQVN
                     GDKQSVNWITKVGDWIEAIPLFIHERVIRKSAMVAGQQVDVDVIETAVDQEAMEAFFR
                     AHAEYWVRNIDEVCDSFAVDIARTLCVERTPALNKAAQELQKKMGLMWGEAMRCVVGD
                     NPKMHRDIAIIAAAVLQSSQQCLPEIAKTVETKQVDRLQAMREVLLPCLSAGKSQGAS
                     CLLSEAAALCGKFSVKGFAEACLNASPKKNQKQLEGTVERLLPHIAKKTRKTPNLHIL
                     TTESAGMTEGYVQSWIEEEMALFNVIRGRGLEQKVAERMALYRDRISAIGQRVIDDFG
                     MSVVVDEVMAEQKLNRAQAALTVIYSYKIVSEEVGRLATLLEHFKTDTQGNLADYSEP
                     QEILDYLEERRPELEELCVPQVVENNGHAFQLKLADWMDVHPDTETSIAERAVIAGDP
                     DYEAELQSLVLSAARSR"
BASE COUNT          290 a          416 c          354 g          344 t
ORIGIN      
        1 cgcgagaacg cgccgctgaa agcaccaggc tctgcaattc cgcttcgtaa tcggggtcgc
       61 cagcaatgac cgcgcgctcc gctattgacg tttctgtgtc tgggtgaacg tccatccagt
      121 cggcgagttt gagttgaaag gcatggccgt tgttctcgac tacctgggga acacagagct
      181 cctcaagttc agggcgtcgc tcttcaagat agtccagaat ctcctggggc tccgaatagt
      241 ccgccagatt gccttgggtg tcggtcttga agtgctccag aagtgtcgca agacgtccaa
      301 cttcttcaga cacgatcttg taggaataga taacggtcag agcagcttgt gcgcggttga
      361 gtttctgttc tgccatgact tcatccacaa caacgctcat gccgaaatcg tcaatgaccc
      421 tctggccgat ggcagagatt cggtcgcggt agagtgccat gcgttctgca accttctgtt
      481 ccagccccct accacgaatc acgttgaaaa gagccatttc ctcttcgatc caactctgca
      541 cgtagccttc ggtcataccg gcagactccg tcgtcagaat gtgcaggttc ggagtctttc
      601 gggttttctt ggcgatgtgg ggcagcagcc tttcgacggt gccttccaac tgtttctgat
      661 tctttttcgg tgaggcattc agacaggctt ccgcaaagcc tttcacactg aactttccac
      721 acaatgcagc agcctcactc aagagacatg aagcgccttg gctcttgcct gcgctgaggc
      781 aaggcaggag gacttcacgc atcgcctgaa gccgatccac ctgtttggtt tcaacggttt
      841 tggcaatctc cggcaggcac tgctgactgc tctgcagaac cgctgcggcg atgatggcga
      901 tgtcacgatg catcttggga ttgtctccca caacgcatcg catagcctca ccccacatca
      961 gtcccatctt cttctggagt tcctgggcgg ccttgttcaa cgcaggcgtg cgctccacac
     1021 aaagcgttcg ggcaatatcc accgcaaagg aatcacagac ctcatcaatg ttgcgcaccc
     1081 agtattccgc atgggcgcgg aagaatgctt ccatggcttc ctgatccacg gccgtttcaa
     1141 tcacgtctac gtcaacctgc tgacctgcaa ccatggcgct ctttcgaatg acacgctcat
     1201 ggatgaaaag tgggatcgcc tcaatccagt cacccacctt ggtgatccag ttcacgctct
     1261 gcttgtcgcc attgacctga acttcgccac tgcctggaca cgaataggtg ttgtgtccac
     1321 ccaaacgaac cagatctcga agatcctctt cttttccggg atgcacgtcg cgaacgctgt
     1381 tggcatgtgc caggttgatc gtct
//
LOCUS       DAOQUC010001252         1404 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_130128_length_1404_cov_2.553002, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001252 DAOQUC010000000
VERSION     DAOQUC010001252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1404
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130128_length_1404_cov_2.553002"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1050
                     /locus_tag="PKH07_17340"
     CDS             <1..1050
                     /locus_tag="PKH07_17340"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNT36756.1"
                     /translation="CDVDPDTLQAVSTQYGVPGFSSHGELLSSGKVDAVVIATPHYFH
                     PPIGIDAFDAGMHVLTEKPMAVTIGAAEKFAAKARESKKVFTIMFQKRFSGAFQTAKK
                     LVEEKAIGELYRVSMIATGFRTQRYYDSGAWRATWLHEGGGVIINQASHELDFFTALV
                     GLPSQVCAKVRTRLHRIEVEDFGHCIMEFPNGAVGYLCISTDEAPGTHRIELCGDSGR
                     ILIDGNKILLSRLDTPSLKDFALQSDTVWGSPKAQTEEVAIPDGKANHGHVLQNFVNT
                     ILGKEELVIDGAVGLTELELGNAIHLSSHIGKAVELPLDRQQVEDHFAQLRRISKPKA
                     SISVARETDPQHLQK"
BASE COUNT          342 a          338 c          392 g          332 t
ORIGIN      
        1 tgtgatgtgg atccggatac tttgcaggct gtgtcgacac agtacggagt gcctgggttt
       61 tcatcccatg gggagttact ctcaagcggc aaggtagacg ctgtagtgat cgccactcct
      121 cactattttc atccgcccat tggcattgat gcctttgatg ccggtatgca tgtgttgact
      181 gaaaagccaa tggcagtcac gatcggcgca gcagagaagt tcgcagcaaa ggcacgagag
      241 tcaaagaagg tctttaccat catgtttcag aaacgctttt ctggagcgtt tcagacagca
      301 aagaagctcg ttgaagaaaa ggccatcggt gagctttatc gagttagcat gatcgctacc
      361 gggtttcgaa cgcagcgcta ctatgactcg ggtgcttggc gcgcgacctg gcttcacgaa
      421 gggggcggcg tcattatcaa ccaagcttcg cacgagctgg acttcttcac ggctctggtc
      481 ggtcttccct cacaggtatg tgccaaagtc agaactcgtc tacaccgcat cgaggttgag
      541 gatttcggcc actgcatcat ggagtttccc aatggcgctg ttggctatct ctgcatcagc
      601 accgatgaag ctccaggaac gcataggatt gaactgtgtg gtgactcggg tagaattctg
      661 attgacggca acaagattct cctaagcaga ctcgacacgc catcgctgaa ggattttgcc
      721 ctacaatctg acaccgtctg gggaagtcct aaggcgcaaa ctgaggaagt tgcgatccct
      781 gacggaaaag caaaccacgg acatgtcttg cagaatttcg taaacaccat ccttggtaaa
      841 gaggaacttg tgatagatgg tgcggtcgga ctgacggagt tggagctcgg aaatgctatc
      901 cacctttcct ctcacatcgg aaaggctgtg gagttgcccc ttgaccgtca gcaggttgag
      961 gatcacttcg cacagttgag aaggatctcc aaaccgaagg cgagcatctc tgttgctcgg
     1021 gagacagatc cacaacactt gcagaagtag ggcatcagag ggataggaca ccctctggct
     1081 caccggagtg ctccaacgcc ttgcttggct taggaagccc gatgggcttc ttcctgaatt
     1141 cggttctgtg tcttgatgac tattccctct tggaatgggc tgaacggctc acttcaagag
     1201 ggatttctct gagctctaga ggaatctgca ttttagggca gtgactgtgt gatgatattt
     1261 gtaagaaggg tttggacgag ccagaggccg aggatggcta cccaaggtga gaagtccatc
     1321 attccgacag cactccaagg gaagaaacgc cgtaccatct ggagaaaagg agatgcaagc
     1381 gcattgagga tttggactga ctca
//
LOCUS       DAOQUC010001253         1404 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_130161_length_1404_cov_2.232024, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001253 DAOQUC010000000
VERSION     DAOQUC010001253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1404
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130161_length_1404_cov_2.232024"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..311
                     /locus_tag="PKH07_17345"
     CDS             <1..311
                     /locus_tag="PKH07_17345"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HNT36757.1"
                     /translation="STSCSFILQVTDDKSPADYEADDVLRAAVERHFEIIGEAMNRIA
                     RYDSEIAMRITDYDRIISFRNVLIHGYDLVDDQAVWSVIRENVPELKKQVQELLKLVD
                     "
     gene            405..722
                     /locus_tag="PKH07_17350"
     CDS             405..722
                     /locus_tag="PKH07_17350"
                     /inference="COORDINATES: protein motif:HMM:NF024546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRL domain-containing protein"
                     /protein_id="HNT36758.1"
                     /translation="MKRVVMSLVVVLLCSGCFIAPVVPPVGIGFSSFKAPLDVENSQT
                     TVSARRGEASSVCVLFLFSFGEAGIEDAARAGGLRVVNHADYEYLNVLGIFQKYTTIV
                     YGE"
     gene            742..1098
                     /locus_tag="PKH07_17355"
     CDS             742..1098
                     /locus_tag="PKH07_17355"
                     /inference="COORDINATES: protein motif:HMM:NF024546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRL domain-containing protein"
                     /protein_id="HNT36759.1"
                     /translation="MKSFLTHVFIVILLLLVCQGCAVVRSAPVVPPQGVLFSRTRAPL
                     SVDFSETPICEKKGSASTLYVHDILLTGLSFAWDDAGIAKAAANGGLREVEYADYEML
                     TILGVFGRFTVHAHGR"
     gene            complement(1187..>1404)
                     /locus_tag="PKH07_17360"
     CDS             complement(1187..>1404)
                     /locus_tag="PKH07_17360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36760.1"
                     /translation="KAGGRVAVYSTLIPDGEMRQAIASGTDIFSRRTPMPPECQTQEQ
                     AIQQLLAQGLITEETARNALTRQGISN"
BASE COUNT          288 a          325 c          418 g          373 t
ORIGIN      
        1 tctccacatc ttgttcgttc atcttacagg tgacagatga caagagccca gccgactacg
       61 aggctgacga tgttcttcgg gcggctgtcg agagacactt cgagatcatt ggtgaggcga
      121 tgaatcgtat cgcacggtac gactctgaga tcgcgatgcg tatcacggac tatgaccgaa
      181 taatctcgtt ccggaatgtt ctcatccatg gttacgatct tgtggatgat caggcagtct
      241 ggagtgtcat ccgtgagaat gttcctgagt tgaagaaaca agtacaggag ttgctgaaac
      301 tcgttgatta gtgttcagta actctgtgat tagatggtag gttcgtggtt ttggtgctgg
      361 cgacgggagt tgtcggcaac gggtatcgaa aggagagcag aagtatgaaa cgggtggtca
      421 tgagtcttgt ggttgttctg ttgtgttcgg gctgtttcat tgcgccagtg gtaccaccgg
      481 tgggcatcgg cttctcgtca ttcaaagcgc cgctggacgt tgaaaatagt cagacgaccg
      541 tcagcgccag gcgaggggaa gcaagcagcg tctgcgtgtt gttcctgttt tcatttggag
      601 aagctgggat tgaagatgca gcacgtgcgg gtggtcttcg ggttgtcaac cacgcagact
      661 acgagtacct gaacgttctg gggatattcc agaaatatac gaccatcgtc tatggcgaat
      721 agccctgagc aggagcgaac aatgaagagt tttctaacac atgttttcat tgtgatcctg
      781 ttgctgcttg tgtgccaggg ttgtgcagtg gtcaggagcg cccctgtggt gcccccgcaa
      841 ggcgttctgt tctccaggac acgagcacct ttgtccgtgg acttctccga gactcccatc
      901 tgcgagaaga aaggctccgc atcaactctg tatgtccatg acattcttct caccgggttg
      961 agttttgcct gggatgatgc gggcattgcc aaggctgctg cgaacggtgg tctgcgcgaa
     1021 gttgagtatg cggactacga gatgctcacc attcttggtg ttttcggacg gttcacagtg
     1081 cacgctcacg ggcgttagag ttctgcaacg caggcatgcc cattacagtg cgttggtgca
     1141 tctaagaacc atatctgttc tgcatcgtgt ctgatgctga gggcttttag tttgatatcc
     1201 cctgtcgcgt gagcgcgttg cgagcggtct cttcggtgat aagcccttga gcaagcagtt
     1261 gctggatcgc ctgctcttgt gtctgacatt caggaggcat cggcgttctt cgtgagaaga
     1321 tatccgtgcc agaggcaatt gcctggcgca tctctccgtc aggaatcagg gtggaataga
     1381 cagcgacccg accgcctgct ttcg
//
LOCUS       DAOQUC010001254         1402 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_130426_length_1402_cov_3.643653, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001254 DAOQUC010000000
VERSION     DAOQUC010001254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1402
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130426_length_1402_cov_3.643653"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..825)
                     /locus_tag="PKH07_17365"
     CDS             complement(<1..825)
                     /locus_tag="PKH07_17365"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNT36761.1"
                     /translation="MMKQRDVVLECRNISHWFGPKRVLFEVNLKVIRGEIVSLVGPSG
                     CGKSTLLRAIIGTHLPCQGEVLIQRSDADTPEPVRRPGRDRGIVYQHYSLFPFLTALE
                     NVAFGLMFSQTSPPYRAFQYLRWRKLRKSHLAEATALLERLRLGDAIHQYPHEMSGGM
                     RQRVAIAQALIMKPEVLLLDEPFGALDEATREELQRMLLTLYLENCQAMKRGEAPPYT
                     FMIVTHELNEAIYVGDRVIGLSQYWDWKSEKHTTCPGATIVYDQVAPVELPDEERNY"
     gene            complement(822..>1402)
                     /locus_tag="PKH07_17370"
     CDS             complement(822..>1402)
                     /locus_tag="PKH07_17370"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNT36762.1"
                     /translation="RLFLGLLIAIVGAIVIGLLMGCFGFLEALLVPPLSLLAKIPPTA
                     ALAVFFVLVGTNMKMFAAMIAFGVLPTLAQSVYLSIRAVPNEMLYKGYTLGASHVEVV
                     WNIIVRIILPDLIDAVRLQIGPAMVYLIAAEMVCADVGFGYRIRLQSRLLNMNVVYPY
                     LGVLAAFGFGMDYGLRLLQRLCCPWFVSRERT"
BASE COUNT          347 a          394 c          369 g          292 t
ORIGIN      
        1 atagtttctc tcctcgtcag gcagttctac aggcgctacc tgatcgtaga caatggtggc
       61 acccggacag gtcgtgtgtt tttcactctt ccagtcccag tactgcgaca acccgatcac
      121 ccgatctccg acgtagatag cctcgttgag ctcgtgggtc acaatcatga aagtgtacgg
      181 tggagcttct ccccttttca tggcctgaca gttttccagg taaagggtca agagcatccg
      241 ctgaagttct tcgcgcgtgg cttcatcgag agcaccaaat ggctcgtcga gcaggagcac
      301 ttcaggcttc atgatgagcg cctgagcgat agcaacacgc tggcgcattc cgcctgacat
      361 ttcatgagga tactggtgaa ttgcgtcacc gagtcgaagt ctctccagaa gcgcggtcgc
      421 ctctgccaga tgggactttc tcagtttcct ccaacgcagg tactggaaag cccggtatgg
      481 cggggaggtc tggctgaaca tcaggccaaa tgccacgttc tcaagagccg tgagaaatgg
      541 aaagagagag taatgctgat agacaattcc ccgatcgcgt cccggccgtc tgaccggctc
      601 cggagtatcg gcgtcagagc gttgaatgag aacctcgcct tgacagggga ggtgcgttcc
      661 aatgatcgct ctgagcaagg tggatttccc acagccgctt ggccccacca gggagacgat
      721 ctcaccacgg atcactttca ggtttacttc gaagagcacc cgcttggggc caaaccaatg
      781 gctgatgttt cggcattcga ggacaacatc ccgttgtttc atcatgtcct ctccctgctg
      841 acaaaccatg ggcagcacag tctttgaagc agtctcagcc catagtccat cccgaaaccg
      901 aacgccgcca gaacacccaa gtaggggtag acaacattca tgttgagaag ccttgactga
      961 agtcgaatcc ggtatccaaa gcctacatcg gcgcagacca tctctgcggc aatgagatag
     1021 accatcgctg gaccaatttg aagacgcacg gcgtcaatga gatccggcag gataatccga
     1081 acaatgatgt tccagactac ttcgacatgc gaagcgccca aggtgtagcc tttgtagagc
     1141 atctcgttgg gaacggctcg aatcgaaaga tagaccgact gtgccagcgt tggcagcact
     1201 ccgaatgcaa tcatggcggc aaacatcttc atgttcgttc caaccaacac gaagaacaca
     1261 gccaacgccg cggttggcgg gatttttgcc agcagggata gcggtggaac cagcaacgct
     1321 tccagaaagc cgaagcaccc catcaacagt ccgataacga tcgccccaac aatggcgatc
     1381 agcagtccaa gaaagagtcg tg
//
LOCUS       DAOQUC010001255         1402 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_130438_length_1402_cov_3.406830, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001255 DAOQUC010000000
VERSION     DAOQUC010001255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1402
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130438_length_1402_cov_3.406830"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..633
                     /locus_tag="PKH07_17375"
     CDS             <1..633
                     /locus_tag="PKH07_17375"
                     /inference="COORDINATES: protein motif:HMM:NF017509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36763.1"
                     /translation="SVRRVILPDLNDELAKNVGPYETLADLKDDVRTRLAAQMKERNR
                     EQTKQNLIGKLLELHQFPMPESLVTSRFEYLSSLQESQFKRYGISQDTLGENREKIDK
                     ANRERAERQTRLSVILADVVERESLILTDDEFDERLEKVAHESGYEPDGFANYVFRKG
                     LDNYYKDLFIEDKALEFLLDKVTLTSSEATTPDSETPDSQTESTSKEAEA"
     gene            731..1342
                     /gene="clpP"
                     /locus_tag="PKH07_17380"
     CDS             731..1342
                     /gene="clpP"
                     /locus_tag="PKH07_17380"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HNT36764.1"
                     /translation="MLIPMVVEQTSRGERAYDIYSRLLKDRIVFLGSAIDDHVANLVI
                     AQLLFLQAEEPDKDIQFYVNSPGGSVSAGLAIYDTLQYIKPNVATTCMGLAASMAAVL
                     LAAGTKGKRYALPNSRILIHQPMGGFRGQATDIDIHAREILKTRENLNRVLAGHTGQS
                     VERIAQDTERDYFMSPEEALEYGIIDEIYRPQLKAKKDKEDKR"
BASE COUNT          370 a          345 c          372 g          315 t
ORIGIN      
        1 tctgttcgtc gggtcatact tcccgatctg aacgatgaac tcgccaagaa tgtcggcccc
       61 tacgagactc ttgctgatct gaaagatgac gtgcgcacga ggcttgcagc ccaaatgaag
      121 gagcgcaatc gcgagcagac caagcagaat cttatcggaa aacttcttga gcttcatcag
      181 tttccaatgc cggagtctct ggttacatca cggtttgagt atctcagttc tttgcaggag
      241 tcccagttca agagatacgg aatcagccag gataccctgg gtgagaaccg agaaaagata
      301 gacaaagcca acagagaaag agcagagcgt caaacacgac tttccgtcat tctcgcagat
      361 gttgtggaga gagaatcact tatccttact gacgatgagt ttgacgagag gctcgagaaa
      421 gtggcacacg aatcagggta cgagcctgac ggctttgcga actacgtttt tcgcaaaggc
      481 ttggacaact attacaagga tctttttatc gaggacaaag ccctcgagtt tcttctggat
      541 aaggtcactc tcactagttc ggaagcaacc actccggaca gcgagacgcc ggacagtcag
      601 acggagagca cctccaagga agctgaggca tagtctcatc ttggtaaacg acgggactat
      661 gggcaaccca tttgtgccca agttcaagac cagtttatca tcgagcggtc ctcaggagag
      721 aaggtaatat atgctgattc cgatggtcgt ggaacagacg agtcgtggag agcgtgccta
      781 tgacatctac tcgcggctct tgaaggatag gatcgtattt cttggcagcg ccattgatga
      841 ccatgtcgca aaccttgtta tcgcccaact gctgtttctt caggctgagg agcctgataa
      901 ggatattcag ttttacgtca acagtccggg gggatccgtc agtgcagggc tcgccatcta
      961 tgacaccttg cagtacatca aaccgaacgt cgccacgacc tgcatgggtc tggctgcaag
     1021 catggctgcc gtgctgttgg ctgccggcac caagggaaag cgctatgccc ttcccaactc
     1081 ccgaattctg attcatcagc cgatgggtgg cttccgtggt caggcaaccg atattgacat
     1141 acatgcccgg gagatcctca agacccgtga gaatctgaat cgggtcttgg caggtcacac
     1201 aggccagtct gttgagcgta ttgcccagga cacggagcgt gattacttta tgtctccgga
     1261 agaagccttg gaatacggca tcatagatga gatttatcgg cctcagttaa aggccaagaa
     1321 ggataaggaa gacaagcggt agtcctgctt cccaacaact agagcacgat ggagctgaca
     1381 tgccaaaaga tgattccgta aa
//
LOCUS       DAOQUC010001256         1402 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_130478_length_1402_cov_2.613215, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001256 DAOQUC010000000
VERSION     DAOQUC010001256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1402
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130478_length_1402_cov_2.613215"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1139
                     /locus_tag="PKH07_17385"
     CDS             <1..1139
                     /locus_tag="PKH07_17385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36765.1"
                     /translation="YSLEEDDVWWRRYDQLDRVDFGMDLEAPFTVFQWLRITPFTGYQ
                     GIFYGTPEQRWSLRNIHGERFDVTDPDDSYDRDFLRRYGDRYYFAGGLRPGWEYESNW
                     DDVMQNVWKLGTEFSTRLVQPLGSTRRFSQMRLVVEPTMTVVGYFASKDLDDEVPQEF
                     LNQTFYPTIPEIDEVDSYRRSARIVRTGLDLKLEGKDYDRVTHRLARLSLSAAKDFHS
                     SDDDDGEYEDFMMELSLTPNKYVEFKHFWLYDLDQSQTAAMWDGLTIIPDDRVKFTVG
                     YAQFEDTHNVMDRESYATFDMKLAMSKKWAMELRERYDLEQSYFRDSRCSFIRDLHDW
                     EAAFSVRNRKRLYRDDEFQITFGMQFKLPTQAAGGFPLADRFVR"
BASE COUNT          301 a          353 c          402 g          346 t
ORIGIN      
        1 aatacagcct tgaagaggac gatgtctggt ggcgtcgcta tgaccaattg gatcgggtgg
       61 atttcgggat ggatctggaa gcgcccttca ccgtgttcca atggcttcgc atcaccccct
      121 tcacgggata tcagggcatt ttctacggga ctcccgaaca gcgatggagt cttcgaaaca
      181 ttcatggcga acgatttgat gtcaccgatc cggacgacag ctacgaccgg gatttcctca
      241 gacgctacgg tgaccgatac tacttcgcgg gtggtcttcg tccgggttgg gaatacgaga
      301 gcaactggga cgacgtcatg cagaatgtgt ggaaactggg gacagagttc tccacacggc
      361 tcgtgcagcc cttgggttcg acacgccgct tctcacagat gcgactggtg gttgagccga
      421 cgatgaccgt cgtaggctat ttcgcgagca aggatttgga tgatgaggtt cctcaagagt
      481 ttctgaatca gactttctat ccgacgattc ccgagattga cgaggtggac agctatcgtc
      541 gttcggcacg aatcgttcga acagggctgg atctcaaact cgaaggcaag gattacgatc
      601 gcgtcaccca tcgtctggcc agactgagtc tttctgcagc caaggacttc cacagctccg
      661 atgacgacga tggtgaatat gaagatttca tgatggaact cagtctgaca ccaaataagt
      721 atgtggagtt caagcatttc tggctttacg atttggatca gagccagaca gcggccatgt
      781 gggatgggct gaccatcata cctgatgacc gagtcaagtt cactgtgggt tacgcccagt
      841 ttgaggacac tcacaatgtg atggatcggg aaagctacgc gacgttcgac atgaaactgg
      901 cgatgtcaaa gaagtgggca atggaactca gggaacgcta cgatctggaa cagtcctatt
      961 tccgtgattc acggtgcagc tttattcgcg atcttcatga ctgggaagcg gccttctccg
     1021 ttcgcaacag aaagcggttg taccgggatg acgaattcca gatcaccttc gggatgcagt
     1081 tcaaactgcc aacgcaggca gctggtggtt ttccacttgc tgaccgcttt gttcgctgaa
     1141 cgcacggcac tttcacgtag cacacattcc ggttgccagg tgcgttcctt tttcccacgc
     1201 ttccaacttg ctcccttggg tgccagccct caggttcgcg aagcggttct gagggttgct
     1261 cttgaaaaga agatcgcctg cccttaactg acccgctcac ggtgttggtg tttccgtcgc
     1321 ggattccgtc ggtgtcgctg ttcgggttgg tgtacgcgtg agagtcggag tccttgtcgg
     1381 ccgtgttggc gttggagtgt tc
//
LOCUS       DAOQUC010001257         1400 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_130782_length_1400_cov_2.947955, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001257 DAOQUC010000000
VERSION     DAOQUC010001257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1400)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1400)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1400
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130782_length_1400_cov_2.947955"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1400)
                     /locus_tag="PKH07_17390"
     CDS             complement(<1..>1400)
                     /locus_tag="PKH07_17390"
                     /inference="COORDINATES: protein motif:HMM:NF012618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HNT36766.1"
                     /translation="ETFYGYTGNVWSASFSPDGRRVVTGQEWPYGEGKVWDADTGIER
                     LTLSASAEYMVSSASFSPDGSRILGGLSSESILWNASTGEEVRRFSGGHPVAYSPKGE
                     TILVNTELYDVATGTRLHEFIGHQYGVTSVAFSPDGDYVLTGSRDQTAKLWKVETGEE
                     LRTFAGHTGGVSSVAFSPDGSKVLTGSADAKAKIWRTDSGSLERTLSGEISPIYAVAY
                     SPDSRFIATGGDAIPYGANVTIWDAVTGEPVRVLRGHSWHIYSVEFSPDGRKLLTGSA
                     DGTALVWEINPPRAIIVAGGGDYAGNGIAQQTNDLGAYAYQTLKRRGYEAQDILYLSA
                     FGPTDPSDSTKPFRDADGDGLNDVDGWATLGALQTALTGEFAQEAGRLMIIMIDHGFI
                     TNSGCLFQMSPTQVLPASTLDNWLDTLQTNSPVDVTLVVDACYSGGFVETCKGGVGGH
                     KRIVISSTTSNNVATFL"
BASE COUNT          328 a          400 c          352 g          320 t
ORIGIN      
        1 agaaaagttg ccacgttgtt ggaagttgtg gaagagatca caatccgttt gtgtcccccg
       61 acgccacctt tgcatgtttc cacaaaccca ccggagtagc aggcatccac caccagagta
      121 acgtcaacag ggctgtttgt ctgcagagtg tcaagccagt tgtccagtgt ggatgccggt
      181 aaaacctgag tgggactcat ctggaaaagg caaccgctat tggtgatgaa cccatgatcg
      241 atcatgatga tcatgaggcg tcccgcttct tgggcaaact caccagtcag ggccgtctgc
      301 aaagcgccca gcgttgccca tccatccacg tcattcagac cgtccccatc ggcatcgcga
      361 aagggctttg tggaatccga aggatcggtc ggtccaaacg cactcaaata cagaatatct
      421 tgcgcttcat acccacgcct cttgagtgtc tgatacgcat acgccccaag atcatttgtc
      481 tgttgggcaa tgccgttgcc cgcatagtca ccaccaccag cgacaatgat tgcgcggggg
      541 ggattgatct cccaaacgag cgcagtgcca tcggcagagc cggttagcag ttttcgaccg
      601 tcgggggaga actcaacaga atagatgtgc catgagtgac cccgcaaaac ccgaaccggc
      661 tcgcctgtta cggcatccca tattgtcaca ttggcaccgt atgggatggc gtctcctccc
      721 gtggcgatga acctgctgtc gggagagtac gcgactgcat agatagggct tatttcgcca
      781 gagagagtgc gttccagact tcccgagtcg gttctccata ttttggcctt tgcatcagca
      841 ctgcctgtca gaaccttgct tccatctggc gagaatgcta ctgaagagac gccgcctgtg
      901 tgaccagcaa atgtgcgcag ttcctcccct gtttcaacct tccacagttt cgcggtctga
      961 tctctgctgc ccgtcaagac gtaatctccg tcaggtgaga aggcaacaga tgtgacacca
     1021 tactgatgtc caatgaactc atgaagcctg gttcccgtag ccacgtcgta gagttccgtg
     1081 tttactaaga tggtctctcc ctttggagag tatgcaactg gatgcccgcc agagaatctg
     1141 cgaacttcct ctcctgttga tgcattccac aagatagact cagaactaag acctcccagt
     1201 atcctcgagc catccgggga gaaggatgcc gaagagacca tatattccgc agaggcagaa
     1261 agtgtcagtc gctcaattcc cgtatcggca tcccagactt tcccttctcc gtaaggccac
     1321 tcctgtccgg taacaactct tctgccatcc ggagagaagg aggccgacca gacgttgcct
     1381 gtataaccat agaaggtttc
//
LOCUS       DAOQUC010001258         1396 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_131374_length_1396_cov_2.805369, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001258 DAOQUC010000000
VERSION     DAOQUC010001258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1396
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_131374_length_1396_cov_2.805369"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1131
                     /gene="bshC"
                     /locus_tag="PKH07_17395"
     CDS             <1..1131
                     /gene="bshC"
                     /locus_tag="PKH07_17395"
                     /inference="COORDINATES: protein motif:HMM:NF021586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis BshC"
                     /protein_id="HNT36767.1"
                     /translation="GTLIGALPVGVVKFAELEKWFRENAQETEFLSEILSLLHETWSA
                     SSRYSEWFNRILHRMLPESGLVIIESALLETKDLTRHFWEPSLREEGLLHRLLAESEP
                     NLRALGIEPRLKCRSEDTGWFMVQNGERHAIHRNENGLAIAGQEVQLETLVADSQTQF
                     KPGAAFRPLIQDSLFPNLATVVGPHELEYHLQLDPLYRHHSVFRPKVLPRFSGAVVES
                     KVARLIKDCNIQPQDFWRPERELEKSLFRLGQGKQITQWADECRNLISEHFQLLAPAV
                     PPLGEDMAAPVEKKCQRMLQEIASLEDILIRRASQRDEILHNRLVRAKSCLFPNGQWQ
                     ERLISPFYFLAKYGLTFLSDLDRAVMQSCCQEPLFHFLEMSL"
BASE COUNT          299 a          382 c          369 g          346 t
ORIGIN      
        1 gggacactca taggagcgct ccctgttggc gtggtgaaat tcgcagagtt ggaaaaatgg
       61 tttcgtgaga acgcgcagga gaccgagttc ttgtccgaaa ttctctcact acttcacgag
      121 acttggtctg cttccagcag atattctgag tggttcaacc gtatcctgca tcggatgctt
      181 ccagagagtg ggttggtcat tatcgaaagc gcgctgttgg aaaccaagga tctgacgcgg
      241 catttctggg agccttccct cagggaagaa ggcttactgc atcgtttgct tgccgagtcg
      301 gaaccgaacc tgagagcgct cggtatcgag cctcgcctga aatgtcgttc cgaagacacc
      361 ggatggttca tggttcaaaa cggagagcgt cacgcaatcc accgaaacga aaatggactg
      421 gccattgccg gtcaggaagt tcagctggag acacttgtag cggattcaca aacgcagttc
      481 aagcccggcg ctgcattccg tccgctgata caggattcgc tgttcccgaa tctggccaca
      541 gtggtcggtc cgcatgagtt ggagtatcac ctgcaactcg atcctctgta tcggcatcac
      601 tctgtcttca gaccgaaggt actgccccga ttctcgggag ccgttgtgga atcaaaagtg
      661 gctcgtctga tcaaggactg caacattcag ccacaggatt tttggcggcc ggaacgtgag
      721 ttggagaaaa gcctctttcg gttgggacag ggcaaacaga tcacccagtg ggcggacgaa
      781 tgtcggaatc tgatatcaga acatttccaa ctcttggccc ccgcagttcc acccttgggt
      841 gaggatatgg ccgccccagt ggaaaagaaa tgccagcgca tgcttcagga aatcgccagt
      901 ttggaggaca ttctgatccg acgtgcctct caacgtgatg agattcttca caaccgcctc
      961 gttcgggcta aatcctgcct ttttccgaat ggtcaatggc aggagcgcct gatttcccct
     1021 ttttacttcc ttgccaagta tggactcaca tttctcagcg acctggatcg ggcagtcatg
     1081 caatcttgct gtcaggaacc cctttttcat tttctggaaa tgtccctctg agtacattcc
     1141 attcggcggt gtagtacgca cttttctgtc catggccatt catggagtga gcagcataga
     1201 gggtggaacg agtctgcttc ggcctccccc gactgcttag tcctttcgga cactcacacg
     1261 ggtttcctcc tgaataccgc tgcagaacgc attcatcctg cccgctgttg ttcgtctttt
     1321 tcgcgggcgg gacgcccgcg cccccttgcc ctcatcccga cctttttcga gaagatctcc
     1381 tgggaacgcc aagtgt
//
LOCUS       DAOQUC010001259         1395 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_131586_length_1395_cov_2.201493, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001259 DAOQUC010000000
VERSION     DAOQUC010001259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1395
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_131586_length_1395_cov_2.201493"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            141..1028
                     /locus_tag="PKH07_17400"
     CDS             141..1028
                     /locus_tag="PKH07_17400"
                     /inference="COORDINATES: protein motif:HMM:NF024790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HNT36768.1"
                     /translation="MMECVQQRRPVTQAVALALFVLPALYVSAILSTAVHEILGHGFC
                     AMLLGGEFSGFVLKWDAMGWAYSELPESAPLSHHIFFSASGIIANLVFGGMLLGFAFI
                     VSPKPHVQLMLLVVSFTCLIDGISYVLWNAYHPDPSGDIGRIIWLLWSGPQPPEDLAI
                     RWVLLVGGALIFFATVFFFSTAVFIRVEAVVLGPDRLKGRSRVLALLLFLVLPSLVGW
                     FSFDWNQLVPGIGLLPNMTGVLCEVTAATLLFWYRPRWTCGNSARPIPVGHILVSWMC
                     LTATVLVVALWATDGVSWR"
     gene            complement(1073..>1395)
                     /locus_tag="PKH07_17405"
     CDS             complement(1073..>1395)
                     /locus_tag="PKH07_17405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36769.1"
                     /translation="QNLTFTQTNEYDPNPRERIKLREWHLTAKTTETRQDMQLVTLYH
                     PYKIERKAEFQATLESSPQGYVLKATPCGSELTALLPADSSAAIKLRLERRGDSAQII
                     ELGE"
BASE COUNT          218 a          380 c          432 g          365 t
ORIGIN      
        1 ttaccatgga cactgaaaaa cgtgattttg acagagaggc gggtgtgcgg gacgagaatc
       61 ctacgcgggt caagctggcg ggtggccgtg agccccgaga tggacgccgc cgacctcggc
      121 tcaggttgta gaggctcgcc atgatggagt gcgtccagca aagacgcccg gtcacgcagg
      181 cggttgcctt ggctctattt gtcctgccgg cgctgtacgt ttcagcgatc ctgagcaccg
      241 ccgttcatga gattcttggg cacggattct gtgcaatgct cctcggtggg gagttctcgg
      301 gattcgtgct gaaatgggat gctatgggct gggcgtatag cgaattgccg gaatctgcac
      361 cgctttcgca ccacatcttc ttctcggctt ccggcatcat cgcaaatctg gtgtttggcg
      421 ggatgctcct gggattcgcc ttcattgtct cgccgaagcc tcacgtccaa ctgatgctgc
      481 tcgttgtctc cttcacttgt ctgatcgacg gcatctccta tgtcctctgg aacgcctatc
      541 acccggatcc ttcgggagac ataggcagga tcatttggct gctgtggagt gggccgcagc
      601 ctcccgaaga cctggcgatt cggtgggtac ttcttgttgg aggagccttg atcttcttcg
      661 ccacagtgtt cttcttcagt accgccgtgt tcatccgcgt ggaagctgtc gttctcggtc
      721 cagaccggct caaggggaga tcgagagtcc ttgcattgct gctctttctc gttctaccga
      781 gtcttgtcgg gtggttcagc ttcgactgga accaattggt gcccggaatt ggtttgctgc
      841 cgaacatgac cggcgttctc tgcgaggtga cggcggcgac gttgctcttc tggtatcgcc
      901 ccagatggac atgcggaaat tcggctcgtc cgattccggt cggtcacatt ctcgtctcat
      961 ggatgtgcct gactgcaaca gtcttggtag tggcgttgtg ggccacggat ggagtctcct
     1021 ggagatagag tacctctttt tctcgccaga tgaactcgga ttggcaccga gatcactcac
     1081 caagctcgat gatctgcgcc gagtcccccc gacgttcgag tctgagcttg atcgcggcgc
     1141 tgctgtcggc cgggagcaac gcggtcagtt cgcttccgca tggggtggct ttcagtacgt
     1201 agccctgcgg cgatgactcc agtgtggctt ggaattccgc cttcctctcg atcttgtacg
     1261 ggtggtagag cgtgacaagt tgcatatcct gccgcgtctc ggttgttttg gccgtcaggt
     1321 gccattcgcg gagcttgatg cgctcgcgcg ggttcgggtc gtattcgttg gtctgggtga
     1381 aggtgaggtt ctgcg
//
LOCUS       DAOQUC010001260         1395 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_131609_length_1395_cov_2.024627, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001260 DAOQUC010000000
VERSION     DAOQUC010001260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1395
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_131609_length_1395_cov_2.024627"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            94..837
                     /locus_tag="PKH07_17410"
     CDS             94..837
                     /locus_tag="PKH07_17410"
                     /inference="COORDINATES: protein motif:HMM:NF012874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HNT36770.1"
                     /translation="MVQFSRGVGFGIARNLDKTNQTIQTALSQLSSGKRLTSFSVDAS
                     GGSLATKLESAYRGLNEQIRAEEDRISMLQIQEGGLGSITDNLQRIRELQVQAGNDTL
                     NTQGQQAIQGEIDQLTQEIQKTLAETEFGGKQLLQAGPELEAFLTAGPAATGDVTATD
                     KVIEEVTAQRSTAGADINAAQSKVNNLQVAFENTLATYSRIADMDIAQGVTQLTNRDI
                     LQQMTMGTLKNFMNISRQKVLGLIGTLGG"
     gene            898..>1395
                     /locus_tag="PKH07_17415"
     CDS             898..>1395
                     /locus_tag="PKH07_17415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36771.1"
                     /translation="MLSAFKTGNGMPWWVSLSHQKGYALRGGSVAQSPKTRGDSVGCV
                     QRSSCQFPHAAHEDCGQNDTNLNLSPIVRKEGDGSSDSALVGQKSNAERTYHLTPREY
                     SPNPPSTFPDASSFVYVFSHEKHNGAIHRIQARSENWQIVSQDVRILTALSQESLALV
                     AVAGES"
BASE COUNT          352 a          353 c          397 g          293 t
ORIGIN      
        1 atcttcctaa gagccaattt ggttatcggt cagagatgaa aaagtcttga gcactttttt
       61 cagtactctg agcgaaaaag aggagggatg aagatggtgc agttcagtcg tggagtgggt
      121 tttggcatcg cgagaaacct cgacaagacc aaccagacca tccagacagc gctttctcag
      181 ctgtcgtctg ggaagagatt gaccagtttc tcagttgatg cgtcgggtgg ctctcttgcg
      241 accaagttgg agtctgccta tcgtgggctg aacgaacaga ttcgcgctga agaggatcgg
      301 atttccatgc tccaaatcca ggagggtggt ctgggctcga ttacggataa tctccagaga
      361 atccgcgaac tccaggtcca ggcgggcaat gataccctga acacgcaggg acagcaggcc
      421 attcagggcg agattgacca gttgactcaa gagatccaga agacactcgc tgaaaccgag
      481 ttcggaggca agcagctgct tcaggcaggg cctgaattgg aggcgttcct gacagcgggc
      541 cccgcggcaa caggcgatgt gacagccacg gacaaagtca ttgaggaagt gacagcgcag
      601 agatccaccg cgggcgcaga catcaacgca gcacagagca aggtcaacaa cctgcaagtg
      661 gcttttgaga acacccttgc tacttactcc cgaatcgcgg atatggatat cgcgcaggga
      721 gttactcagc tgaccaacag ggacattctt cagcagatga ccatgggaac tctgaaaaac
      781 ttcatgaaca tcagcagaca gaaggtcctg ggtctgattg gcactttggg cggctgagag
      841 cgttttgacc gagagactgt ttctcgatga aatcctagca ccgcatgagg tggtgccatg
      901 ttgtcggcat ttaagacagg caatggaatg ccttggtggg tatctttgtc ccatcaaaaa
      961 gggtatgccc tgcggggagg gagtgttgcc cagtctccca aaaccagggg agattctgtt
     1021 ggatgtgtcc agcggagcag ttgccaattt ccgcacgcag cacacgaaga ttgcggacag
     1081 aacgacacca acctcaacct ttcgccaatt gtccggaaag aaggtgacgg ttcctcggat
     1141 tctgctctcg ttggacagaa gagcaatgcc gaacgcacct accatctgac ccctcgcgag
     1201 tactcaccta atccgccaag cacttttccc gacgcaagct cgtttgtgta cgttttctcg
     1261 cacgagaagc acaacggcgc aattcacaga atccaggcgc gttcagagaa ttggcagatc
     1321 gtctcacagg atgtccggat tctgacggcc ctcagccaag agagtcttgc tctggtggcg
     1381 gtggctggtg agtca
//
LOCUS       DAOQUC010001261         1394 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_131661_length_1394_cov_3.439134, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001261 DAOQUC010000000
VERSION     DAOQUC010001261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1394
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_131661_length_1394_cov_3.439134"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            161..991
                     /locus_tag="PKH07_17420"
     CDS             161..991
                     /locus_tag="PKH07_17420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate hydrolase family protein"
                     /protein_id="HNT36772.1"
                     /translation="MAFTRQQVLERLRKRIAAGVPIIGGGAGNGISAKCQEAGGVDLL
                     IIYNSGRFRMNGRGSLAGCMPYGDANAIVMQMAGEVLTIVEDTPVLAGVCGTDPTRLM
                     DVFLKQVRDAGFSGVQNFPTVGLCDGQFRANLEETGMGYGLEVEMIRQARELDLFTTP
                     YAFNVEEAGAMAKAGADVVVAHMGLTTKGAIGAKTSLTLEQCPVAVQAICDVAKSVNP
                     EIIVLCHGGPISMPEDAEYVLKNTKGVDGFYGASSVERLPAEIAIKEQMQRFKAIRFR
                     "
     gene            complement(1002..>1394)
                     /locus_tag="PKH07_17425"
     CDS             complement(1002..>1394)
                     /locus_tag="PKH07_17425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HNT36773.1"
                     /translation="LFFWGVAWLLLGLVFEPYEGGIKKDRATMSYYFVTTGLAIFTLI
                     AFMIVIDVWNKKRWAQIFVHNGQNPMIAYVAIPCLLPPLLALTPLGHWFGYLARPPWL
                     GALGAALKTLLLALFVQWLTRKKVFWRT"
BASE COUNT          349 a          339 c          410 g          296 t
ORIGIN      
        1 aaaaggcagt tgagatgatg ttggagctca tccgacaggc gaaatcctaa tgtcgttccg
       61 aacgcagcga gtcttcgagc ggcttcagaa tccctcttgg attcacgcga tacagcgaca
      121 ggtgacaaat acatcgaatt atcacgaaag gagtagttga atggctttca cacgacaaca
      181 ggttttggag cgactcagaa agcgcattgc ggcaggagtg ccgatcatcg gtggcggtgc
      241 aggcaacggc atcagtgcca agtgtcagga agcaggcggc gtggacttgc tcatcatcta
      301 caactctgga cgatttcgga tgaacggcag aggatctcta gcgggctgca tgccctatgg
      361 cgacgccaat gcgattgtca tgcaaatggc tggggaagtg ctgaccatag tggaagatac
      421 gcccgtgctt gctggagtgt gtggaacgga tcccacacgt ttgatggatg tgtttctcaa
      481 gcaggttcgc gatgctggat tttctggcgt tcagaacttc ccgaccgttg ggctatgcga
      541 cggacagttt cgcgcgaacc ttgaagagac cggaatgggg tacggcttgg aagtggagat
      601 gattcgtcag gcacgagagt tggatctgtt cacgacaccc tatgcgttca acgtcgagga
      661 agccggggca atggcaaagg ccggagccga tgttgttgtt gcgcacatgg gcttgacaac
      721 caagggagcc atcggagcca agacgtcgct gacactcgaa caatgcccgg ttgcagttca
      781 ggcgatttgc gatgtggcaa aatcagtcaa tccggagatc attgttctct gtcacggcgg
      841 tccaatatcc atgccggaag atgctgaata cgtattgaag aacaccaaag gcgttgatgg
      901 gttctatggt gcatcaagtg tcgagcgact gcctgcggag atcgccatca aggagcagat
      961 gcagcgattc aaggcaattc gtttccgtta gaccatgggc gtcatgtgcg ccagaagacc
     1021 ttcttgcgcg tgagccactg cacaaacagt gccaagagca gcgttttcaa ggctgctccc
     1081 aacgctccca gccatggggg acgagccaga tatccgaacc agtgacccaa cggtgtgagt
     1141 gcgagcaaag gtggcaacag gcacgggatc gcaacgtagg caatcatggg attctgtcca
     1201 ttgtgcacaa atatctgtgc ccagcgtttc ttgttccaga cgtcaatgac tatcatgaaa
     1261 gcgatcaggg tgaagatcgc caagccagtt gtgacaaagt agtaactcat cgtggcgcga
     1321 tccttcttga tgccgccttc gtagggttcg aatacaagcc ctaacagcag ccatgcaacc
     1381 ccccagaaga aaag
//
LOCUS       DAOQUC010001262         1392 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_132022_length_1392_cov_2.325355, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001262 DAOQUC010000000
VERSION     DAOQUC010001262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1392
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132022_length_1392_cov_2.325355"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1307)
                     /locus_tag="PKH07_17430"
     CDS             complement(<1..1307)
                     /locus_tag="PKH07_17430"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009507862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HNT36774.1"
                     /translation="MPMNLGVQYYRAPFPEEKYWDDDFSKIRDSGMNTVQLWVLWGWV
                     EAKPGQFRFEDYDALVELADKKGLGVILSTIAEIQPYWIHREIAGSEMIDHMGRVVVS
                     SNRQECHFGITPGGCTDHPKVWERMSQFLEAVVKQYRNAPNLKGWDAWNELRWNVQSD
                     GRVCFCPHTLERFRRWLDVKYGGLDGLNRAWKRRYDAWDEVWAGKMTGRPYTEMMAFE
                     HFTTHRSDQHGIDRYKLMKPLDPNHPITVHAGAPAPLMPGWSECSAIDRGNDWFFADE
                     LDGVGTSSFPKWGNIDDADFGMRIECVYSAAQGKRVWLSEVQGGRASTAFTPHVPVDA
                     LSQQRWVWNGLACGADTILFWCWRDEVFGHESAGFGIDGLDGLAEERLAAMKVSGDLI
                     EKHEALLESYKPGKPEVGVLFSPQAYYYYWAQEASGGRAAQGL"
BASE COUNT          320 a          429 c          341 g          302 t
ORIGIN      
        1 tgaagcccct gagcagcgcg tcctccagag gcttcctgcg cccagtaata atagtacgcc
       61 tgcgggctga acagcacgcc gacttccggt ttgcccggct tgtaagactc cagcagtgcc
      121 tcgtgcttct cgataagatc tccggacact ttcatcgctg cgaggcgttc ctctgccaga
      181 ccgtccaagc cgtcaatgcc aaatccggcg gactcatgcc caaaaacttc atcccgccaa
      241 caccaaaaca gaatcgtatc cgcgccacac gcaagtccgt tccagaccca tcgttgctgc
      301 gacagtgcat ccaccggcac atgcggagtg aatgccgtcg aggcgcgacc gccctggact
      361 tcgctgagcc agacgcgctt cccctgagcg gcggaataca cgcattcaat ccgcattcca
      421 aagtccgcat catcaatgtt tccccatttt ggaaaactcg atgtgccaac gccgtccagt
      481 tcatcggcaa agaaccagtc gttgcctcgg tcaatggcgg aacactccga ccatcctggc
      541 atcaagggag cgggggctcc tgcatgaacc gtaatcgggt gatttggatc taggggtttc
      601 atgagtttgt atcggtcaat gccgtgttga tcggagcgat gcgtggtgaa gtgctcgaac
      661 gccatcatct cggtgtaagg tcgtccggtc atctttccag cccagacttc atcccaagcg
      721 tcatagcggc gcttccacgc acggttcagt ccatccaaac cgccgtactt cacatccagc
      781 caacgtcgaa atctctccaa cgtgtgcgga cagaaacaga cccgaccatc cgactgaacg
      841 ttccagcgca gttcattcca agcgtcccac cctttcagat tcggagcgtt tcggtattgc
      901 ttgacaacag cttcgagaaa ctgtgacatg cgttcccaga ctttgggatg atcggtgcag
      961 ccgccgggag tgatgccgaa gtggcattcc tggcgattgg acgagacgac aacgcgtccc
     1021 atgtgatcga tcatttcact gccagcaatc tctcggtgaa tccaataggg ctggatttcc
     1081 gcgatagtgc tgagaatgac accaagacct tttttgtccg ccaactcgac caaagcgtca
     1141 tagtcctcaa accgaaactg tcccggcttg gcttccaccc aaccccagag cacccacagc
     1201 tgaaccgtgt tcatgccgga gtccctgatc ttcgagaaat catcgtccca gtatttctcc
     1261 tcaggaaacg gagcgcgata gtactgaaca ccgagattca tgggcatgtt gtatagtcct
     1321 ttctcacaaa agaattctga caaacttcgg ggcttcttat atcacacatc gggcggacgg
     1381 gtcgagaacg ca
//
LOCUS       DAOQUC010001263         1391 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_132121_length_1391_cov_3.020958, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001263 DAOQUC010000000
VERSION     DAOQUC010001263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1391
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132121_length_1391_cov_3.020958"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            90..515
                     /locus_tag="PKH07_17435"
     CDS             90..515
                     /locus_tag="PKH07_17435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HNT36775.1"
                     /translation="MARFPGISDAASLALHALSLFAKKHAILLSTREVAELLDVSEAH
                     LAKVLQRLNRSGLVVSVRGPGGGFTLAKPAEKVTLLEVYEAIEGPMDQSTCLLRKPVC
                     PEGKCILGDLLKSMNAQVKDRLSQTTLADAAGSFGSERE"
     gene            518..1258
                     /locus_tag="PKH07_17440"
     CDS             518..1258
                     /locus_tag="PKH07_17440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005931734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNT36776.1"
                     /translation="MKRKIVRIDEDKCNGCGLCASACAEGAIEIVDGKAKLVGEILCD
                     GLGACLGTCPQDAITIEEREAEAFSEAAVHQRSRNNEQQSAVPKAGAGFVCPGRQAQQ
                     IERSGNRAQTSSSLVQPSELTNWPVQLKLVPAQAPYFDGCDLLVAADCTAFSYGDFHR
                     GFLKGRVLLVGCPKLDDVELYRDKLSQILAANDIRSIEVLFMEVPCCFGLTRLVDDAL
                     SLSGKDIPTKLTKISLRGEVLETRVREI"
BASE COUNT          361 a          311 c          407 g          312 t
ORIGIN      
        1 aaaagatcaa aaaaatgcga taccgagctt gacaacgaaa cgtaaatagg tatagtgata
       61 ccagaatact taaatagatg aggagtataa tggcacggtt tccaggaatc tcagacgcag
      121 caagccttgc gcttcacgcc ctgagtctgt tcgcaaagaa gcatgcgatt ctgctttcaa
      181 ctcgagaggt ggcggagctc ctggacgttt cagaggcgca tttggcgaag gtgctccaaa
      241 gactcaaccg ttccggtctt gtcgtctcag tgagggggcc tggtggtgga ttcactctgg
      301 caaagccggc cgagaaagtg actttgctgg aagtctacga agccattgaa ggaccaatgg
      361 accaaagtac ctgcctcttg aggaaaccag tctgcccaga agggaagtgc atcctgggcg
      421 atcttctgaa gtccatgaat gctcaagtga aggacagact gtcgcaaaca acccttgcag
      481 acgctgctgg gtcattcggg agtgaaagag aatgaagatc aagcgaaaga ttgtcagaat
      541 tgatgaagac aaatgcaacg gatgcggtct gtgtgcttca gcctgtgcgg aaggtgccat
      601 cgagattgta gacggcaagg cgaaactcgt tggtgagatt ctgtgcgacg gactcggtgc
      661 ctgtctggga acctgtcccc aagatgcaat taccatcgag gaacgagaag cggaagcttt
      721 cagcgaagcg gcggttcatc aacgctcgcg aaacaatgag cagcagtccg ccgtaccaaa
      781 ggcaggagcg ggttttgtct gtcctgggcg acaggctcaa cagatagagc gttcggggaa
      841 tcgcgcccaa acctcatcca gtttggtgca gccttccgag ttgaccaatt ggcctgttca
      901 actgaaactt gttcccgccc aggctccata ctttgatgga tgcgatcttc tggtcgcagc
      961 agactgtacg gcgttttcat atggagattt tcatcgagga tttctcaagg ggagggttct
     1021 gctggtcggg tgtcccaaac ttgacgatgt cgaactgtac cgggacaaac tcagtcagat
     1081 tcttgcagcc aatgacatca ggagtatcga agttttgttc atggaagttc cttgctgttt
     1141 cgggctgacg aggctcgttg acgatgccct cagcctttcc ggaaaagaca ttccgacgaa
     1201 actgaccaag atcagtttgc gtggtgaagt gttggaaacg agagttcgag agatctgaga
     1261 ggcgacgaaa ggagtctctt tggtgggtcg ttgagccacc ctgaatccca atgacagagg
     1321 tggcgcgaaa agatcgaaat ggaggacggt aagatgtttt gttatcagtg tgagcagacg
     1381 gcaggtggca a
//
LOCUS       DAOQUC010001264         1391 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_132133_length_1391_cov_2.764222, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001264 DAOQUC010000000
VERSION     DAOQUC010001264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1391
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132133_length_1391_cov_2.764222"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1192
                     /locus_tag="PKH07_17445"
     CDS             <1..1192
                     /locus_tag="PKH07_17445"
                     /inference="COORDINATES: protein motif:HMM:NF012903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HNT36777.1"
                     /translation="IETQAWVTTGGGAPTGGVDLKSIDDFNGSPIATGDTITVTGTVA
                     DGTAFTFSYTVASGADTLATFITALETAINGAVAASGDDVTITLLSGGELQIEENVLN
                     VGSQLAISAFTVAGNSVTSTTTAAAAAVTASNAITSTATAAAAGSAVVSVGGGPAQTV
                     AHSSTVTFYGPQPTNELDPTPQITIQFGILSAGTDTLAVVQQEWSGSLDGGKAVTFQN
                     GDQEVLFKNGTSAGFESGEYALINFGAALTAGTVQIAVENDGLNFHIGANEWQNITVG
                     IGDLRASNLGRLQNVGETVADINVKTVTGANLAIQIVDSALEQVSRQRSYLGAITNRL
                     EKTINNLGVSSENLTASESRIRDADVAFETTRFTRNQILLQAGTAILAQANIAPQSVL
                     QLLG"
BASE COUNT          305 a          391 c          410 g          285 t
ORIGIN      
        1 cattgagacg caagcttggg ttactactgg tggtggagct ccgacaggcg gagtggatct
       61 gaagtctatt gatgacttca atggttcccc gatcgcaacg ggtgatacga tcacggtcac
      121 cggcacagtg gccgatggca cggcgttcac cttctcgtac acggtggcga gtggtgctga
      181 cacactggca acgttcatca cggcacttga gactgcgatc aatggtgccg ttgccgcctc
      241 cggcgacgat gtgaccatca cgcttctcag cggtggtgaa ctgcagattg aagagaatgt
      301 cctgaatgtc ggctcacaac tggcgatcag cgccttcaca gtcgctggaa attcagtgac
      361 gtccacaacg actgctgcag cggccgcggt gaccgccagc aatgccatca cgtccacagc
      421 aacggcggca gccgcaggca gtgcggttgt gtccgtgggt ggtggccctg cccagacagt
      481 ggcacacagc tcaacagtaa ccttctacgg gcctcagcca acgaacgagt tggatccaac
      541 gcctcagatc accattcagt ttgggattct gagcgcaggc accgacacgc tggccgtggt
      601 tcagcaagag tggtctggtt cgctggacgg tggcaaggcc gttacattcc agaatggcga
      661 ccaggaagtg ctgttcaaga acggcaccag tgctggtttt gagtcaggcg agtatgcctt
      721 gatcaacttc ggtgctgccc ttacggcggg tacggttcag atcgctgtcg agaacgatgg
      781 cctgaacttc cacatcggtg ccaacgagtg gcagaacatc accgtcggaa tcggcgacct
      841 gagagcctcg aacctcggac gtctccagaa cgttggcgag accgttgcgg atatcaatgt
      901 caagacggtc accggagcca acctggcgat ccagatcgtg gactccgcct tggagcaggt
      961 ttcccgacag cgttcctatc ttggagccat caccaaccgg ttggagaaaa ccatcaacaa
     1021 cctgggtgtg tcttcggaga acctgacggc ttccgagtcc cgaattcgtg acgcggacgt
     1081 tgcctttgag acaacacgct tcacccgcaa ccagattctg ttgcaggcgg gtacggcaat
     1141 tctggctcag gcgaacattg cgcctcagtc agtgctgcaa ctgctcggat aatgactgtc
     1201 gcggcatagc ccgcacaggg ggatcggcat acaagatgac cgatttccat ccctggggag
     1261 agggatttcc ctctccccag ggagagtccc cgagcacggt ttgttagaac ttgaaaggaa
     1321 gtgattttca tgcagatgac gagtgtagaa acgcctattc gtccagcctc gcaggccgta
     1381 cctgcaatgg a
//
LOCUS       DAOQUC010001265         1391 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_132136_length_1391_cov_2.741766, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001265 DAOQUC010000000
VERSION     DAOQUC010001265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1391
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132136_length_1391_cov_2.741766"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1224)
                     /locus_tag="PKH07_17450"
     CDS             complement(<1..1224)
                     /locus_tag="PKH07_17450"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HNT36778.1"
                     /translation="MPRTQWSVLLKWLCTKYFRPYVPLLGLTFACMLMASVFNAGSLA
                     FIKPVLEVILIPEKAETAISENSTSDTLNESKEITPHKLRNPGKLDHLLRPLNQFIIE
                     HSKGRPFGMLCVVAVLLVFVFLLKALFTFLQDYFMRYISEGVIRRLREDVYRHILELP
                     LSHFTGTGTGKLMSFLNYDIVLCKDVVNVVFERAIFQPLQILALLGWAMFLNWQLTLA
                     SVVVIPVSGIVVSVVGKKLRRARFKSQQKLSDLNSILHETFSAVRIVRAFRMEDYERS
                     RFESKTREIFRIAIKMARVRSAAGPAVEWLGALGVGLILLLGGYIVIIRKSMDGSDFM
                     TYILALGMMYAPLKRLVKANSEFQEGLAGAERVHSLLHEEIPIRNDPSLPVLPPFEKG
                     ISYENVSFAYSANAEK"
BASE COUNT          366 a          350 c          352 g          323 t
ORIGIN      
        1 tttctcggca ttggcgctat aggcgaacga cacgttctca taggagatgc ctttctcaaa
       61 cgggggtaat actggaagac tgggatcatt gcgaatggga atctcttcgt ggaggaggct
      121 atgtacacgc tctgcaccag ccaaaccctc ctgaaactcc gaattggctt tgacaagacg
      181 tttcagggga gcgtacatca tccccaacgc cagaatatag gtcatgaaat ctgaaccatc
      241 catgctcttt cggatgatga cgatgtatcc ccccagaagc aggatgagac ctacaccaag
      301 agcgcccaac cactccaccg caggccctgc ggcggagcga acccgagcca tcttgatggc
      361 gattcggaat atctcacggg ttttggactc gaaacgactt cgttcgtaat cctccatccg
      421 gaatgctcga acgattcgca cggcagagaa tgtttcgtgg aggatagagt tcagatcgga
      481 gagtttttgt tggctcttaa agcgcgcacg tcgcagtttc ttcccgacaa cagaaacaac
      541 aatgccgctg accggaatca caaccactga ggccaacgtc agttgccagt tcagaaacat
      601 tgcccaacca agcaaggcca aaatctgaag aggctgaaat atcgccctct caaaaacgac
      661 gttgacgaca tccttgcaga gcacaatatc gtagtttaga aaactcatga gcttcccagt
      721 tcccgttcct gtaaaatggg agagaggcaa ctcgagtatg tgacggtaga catcctctcg
      781 aagtctccgt atgaccccct cactgatgta tcgcatgaag tagtcctgga ggaaagtgaa
      841 aagtgccttg agtaggaaga caaaaaccag aaggactgcc accacacaga gcatcccaaa
      901 aggacgcccc ttggagtgct cgattatgaa ctgattcaga ggcctcagga gatgatcaag
      961 tttgccagga tttcgaagct tgtgtggggt gatttctttg ctctcgttga gggtatccga
     1021 agtggaattc tcggatatcg ccgtctctgc tttctcgggt atgaggatga cctcaagtac
     1081 gggcttgatg aaagcaaggg aacctgcgtt aaagacagaa gccatcagca tgcaggcaaa
     1141 agtcagtccg agaagcggga catagggtct gaagtatttt gtgcagagcc acttgagaag
     1201 cacagaccac tgcgttcgtg gcacattcct ctcgcgaggt tccttcagtt tctttttagt
     1261 ggggcgaaat tcttccatag ccatacaatt gttctcaatc cgttgtcgtt cttcttcttc
     1321 ttcagcatcg tgacagcatg gacgcgggcg atcaactctt tcagggcttt tcgggaaaga
     1381 cccgaagctg c
//
LOCUS       DAOQUC010001266         1390 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_132256_length_1390_cov_3.626966, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001266 DAOQUC010000000
VERSION     DAOQUC010001266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1390
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132256_length_1390_cov_3.626966"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1150)
                     /locus_tag="PKH07_17455"
     CDS             complement(<1..1150)
                     /locus_tag="PKH07_17455"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNT36779.1"
                     /translation="MAEVTLVNPPLDEACRRLMPVPGIPIGLCQLAASLRLRGHKVAL
                     VDCQALDLDPQAGVEQILSLGSRYVGFTGNSLNIDSAAQMAGMCQAKGLKTILGGVHV
                     TAKPTETMFNYPQFDLALMGEGDVRLPDLIEALERGKPIASVPGAVYRDNGNINEIPR
                     GAFVRDLDELPLPAWDLLPDFAQYYQPPARWEIPRPSAILVTSRGCPLPCVFCNKSVF
                     GRQRRFHSPRHIINMIRHLISEHGIKHIAFEDDTLVINKHHVEQLCRGLSESKLGITW
                     SCRVKSDLLHPDLPAFMKRAGCCQVTMTHLSGSQRILDALNTKMTVEDIEEALGLLQK
                     SGIKVLGQFVVGGFEESEETMRESASLIQQVPFDDVDIQFFNPLPGCEA"
     gene            complement(1157..1327)
                     /locus_tag="PKH07_17460"
     CDS             complement(1157..1327)
                     /locus_tag="PKH07_17460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HNT36780.1"
                     /translation="MVKEELLKILACPVCKDKVEYDKKKNEIVCTVCGRRYPVEDGIP
                     IMLVERAEKGDS"
BASE COUNT          326 a          376 c          351 g          337 t
ORIGIN      
        1 gagcctcgca tccgggaagg ggattgaaga actggatatc tacatcatcg aaggggacct
       61 gttgtatgag tgatgcggat tcgcgcattg tttcttccga ttcctcgaag cctccaacga
      121 caaactgtcc cagtaccttg atgccacttt tctggagcaa gccaagagct tcttcgatat
      181 cctcaactgt catcttcgtg ttgagtgcgt cgagaattct ctgagagcct gagagatgag
      241 tcatcgttac ctgacaacag ccagccctct tcatgaatgc gggaagatcc ggatgcagga
      301 gatcggactt cacccgacaa gaccaggtga ttcccaactt gctctcagaa agccctctgc
      361 acaactgctc cacatgatgc ttgttgatca caagggtatc atcttcaaag gcgatgtgct
      421 tgatcccgtg ttcgctgatc agatgccgaa tcatgttgat gatgtgtctg ggactgtgaa
      481 agcgacgctg cctgccaaaa acactcttgt tgcagaatac acagggaaga gggcatcctc
      541 tcgatgttac aaggattgct gacggacgcg gaatctccca acgggcggga ggttgatagt
      601 actgggcaaa gtcaggcaat aaatcccatg cgggcagcgg cagttcatca agatctcgaa
      661 cgaaagctcc acggggaatc tcattgatgt tgccgttgtc gcgatagacc gcacctggaa
      721 ccgatgcgat gggttttcct cgctccaatg cctcgatcag gtcgggcagt cgcacatcac
      781 cctctcccat aagtgcgaga tcgaactgag ggtagttgaa catcgtctcg gtcggtttgg
      841 ctgtcacatg cacaccaccc agaatcgtct tgagtccctt ggcttggcac atacctgcca
      901 tctgggcagc agagtctatg ttcagggaat tgccggtgaa gccgacatat ctgctcccca
      961 gggacagaat ctgctcaaca cccgcttggg ggtctaagtc cagcgcctgg cagtcaacaa
     1021 gggcaacctt gtgccctcga agacgcaagg acgcagccaa ctgacataag ccaattggga
     1081 tgcctggcac cggcatcaga cgacggcacg cttcatcgag tggtggattg accagagtta
     1141 cctctgccat cgtagtctac gaatcacctt tctctgcgcg ttcgaccaac atgatgggaa
     1201 ttccgtcctc taccggataa cgtctgccac agacggtaca gacaatctcg ttctttttct
     1261 tatcgtactc gaccttgtcc ttgcagacgg ggcaagcgag aatcttcagc aactcttctt
     1321 tcaccatttt ctcaatctcc tcgtttcttt gtgagcgttt acattttgct ggcaaagcag
     1381 tcaaagttcg
//
LOCUS       DAOQUC010001267         1388 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_132594_length_1388_cov_2.645161, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001267 DAOQUC010000000
VERSION     DAOQUC010001267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1388
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132594_length_1388_cov_2.645161"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1388
                     /locus_tag="PKH07_17465"
     CDS             <1..>1388
                     /locus_tag="PKH07_17465"
                     /inference="COORDINATES: protein motif:HMM:NF012956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HNT36781.1"
                     /translation="RFTSQYNTSSWSAANIINGQLYGNSGQSWSSSGIGSNATMEFVI
                     DLAGDESVDINRVVFYNYGQGSSNYYSKDFEILVSDTDSANESFSLVIPGTLANVEGP
                     QEFVFPSTQKAKYVLLRILSGYRTDYYELGEFEVYAAGAVGEFYSKALGGSEITNFNV
                     AKWFSTVPAGTSLSLFLRSGNSLPPSDSAWSEWVQMDQGSPVPEELNAQMIQYRIKMT
                     ADSADRSPIFHEMNISSVGTYGTWSLPNVAIQSGVNEITAFAIDPDDRKSPESLPVEV
                     SLDVLQISVAVNPTMVEHDRDVNVAIQNLAYRTGKDDQSKDKRDLKQAQTFIAYFQML
                     DSNGAVVNAKPDGTLSNELLQVPLWFDDALPMSATQEGDFVWDGSLAYLSSLSHTASA
                     TTGLSWHGFTAAASTTFVPDIHSDIVQYVYLDSTLVPREIMIEFETLGGDREHRAFWG
                     RDLIALGTPNTA"
BASE COUNT          312 a          362 c          376 g          338 t
ORIGIN      
        1 gaggttcaca agccagtaca acacctcatc ctggtcggcg gccaatatca tcaacggtca
       61 gctctatggc aacagcgggc agagttggag ctcttcaggg attggttcta acgccacgat
      121 ggaatttgtc attgaccttg ctggagatga atccgtggat atcaaccgag ttgtcttcta
      181 caactacggc cagggttctt ccaactacta cagcaaggac tttgagattc tagtgtctga
      241 cacggattct gccaatgaga gtttcagtct tgtgattccc ggcactcttg cgaatgtcga
      301 aggacctcag gagtttgtct tcccctcgac acagaaagcg aaatacgtgc tcttgcgcat
      361 tctgagcggg tatcgaacgg actactatga gctgggagag ttcgaggtct atgctgccgg
      421 tgctgtggga gagttctact ccaaagcact gggcggaagt gagatcacca acttcaatgt
      481 tgccaagtgg ttcagtacgg ttcctgccgg aacgtctttg tctctattcc tgcgttccgg
      541 gaactctcta ccaccttctg acagcgcctg gagcgaatgg gtgcagatgg atcagggttc
      601 ccccgttcct gaggaactca acgcgcagat gatccagtac cggatcaaga tgaccgctga
      661 cagcgcggac agaagcccca tattccatga gatgaacatc tcatctgtag gaacctatgg
      721 aacgtggagt cttcccaatg tggcgataca gtctggtgtc aatgagatca cggcctttgc
      781 cattgatcct gacgacagaa agagccccga gtcgctgccg gttgaggtat ctcttgatgt
      841 gctccagatt tccgttgcgg tcaatccgac aatggtcgag cacgatcggg atgtcaacgt
      901 cgcgattcag aatcttgcct ataggacggg caaggatgac cagagcaagg acaaacgcga
      961 tctcaaacag gcacagacgt tcattgcgta cttccagatg cttgactcga acggtgcagt
     1021 tgtcaatgcc aagccggatg gaacgctttc gaacgaactg ctgcaagtgc cactctggtt
     1081 tgacgacgcg ctgccgatga gtgcaaccca ggaaggtgac tttgtgtggg atggttctct
     1141 ggcctatctg agcagccttt cgcacactgc ttctgcgaca actgggctgt cgtggcacgg
     1201 tttcaccgct gccgcttcaa cgacctttgt tcccgacatc cacagtgaca ttgtgcagta
     1261 tgtctatctg gattcaactc tggttccacg agagatcatg attgagtttg agactcttgg
     1321 tggcgaccgt gagcaccgcg ctttctgggg gcgcgacctc atcgcccttg gaactccgaa
     1381 cacggctt
//
LOCUS       DAOQUC010001268         1387 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_132758_length_1387_cov_2.578829, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001268 DAOQUC010000000
VERSION     DAOQUC010001268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1387
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132758_length_1387_cov_2.578829"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            287..>1387
                     /locus_tag="PKH07_17470"
     CDS             287..>1387
                     /locus_tag="PKH07_17470"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HNT36782.1"
                     /translation="MVCSSRWLVVLGLVMMLLAYLVPCVCQAGAPVVTNVVAYQRTTG
                     GKEVVVTYDLHDPDSDPCTVTLDFVLPTGEVIRPQLLTGQYGVGIASGTGKQIVWDAM
                     REQPGRYNENCRARVTADDGQGETNEMVYYLPNSQEPVALVRVPAGDFAMGSTVYTDE
                     QPVHTVYLDEYWIMKYPLTVGQWRVYAQANGLAMPSEPGWGGSYTGYMTDTNFNKYPI
                     VNVSWNDVQAFALWSGLKLPTEAQWEKAARGTDSRRYPWGAEEPDSGGLYRANLSGSG
                     DGYAYTSPVGTYGLGVSPYGAYDMAGNVWEWCADWYSATYYSQTPPGGWVNPLGPTSG
                     SYRVLRGGSWNNDAGSARCSARYNGTPTVRDDY"
BASE COUNT          308 a          328 c          449 g          302 t
ORIGIN      
        1 gcgaacaggg cagagaagta gcggacaaga tacccactcc cccttcgaaa acaggtgcga
       61 aaattcagta gtcacgaatc gcttgacaaa accaaatcct ctgtcgcaaa cttatacaca
      121 ctcttggctt ttctggaaaa cgtatttgtt tcacggaacg caaaggatag acgcagggtg
      181 gagacaacgt ggaattcagg aagtggtcgg atttgccctg cgccaaagac gggatggcgt
      241 tgaagaatcg cggcataaat gccaccgtac tataggaggt tcagaaatgg tttgttcatc
      301 gaggtggttg gtggtgttgg gtttggtcat gatgttgctg gcatacttgg ttccgtgcgt
      361 atgtcaggca ggtgcgccgg tggtgacaaa tgttgtggcg taccagcgga caacgggtgg
      421 caaagaggtt gtcgttacct acgatctcca tgatccggac agcgacccat gcacggtgac
      481 gcttgatttc gtgctgccga ctggggaggt catacggccg caactgctca cagggcaata
      541 cggtgttggg atagcgtccg gtaccggcaa acagatcgtc tgggatgcca tgagggagca
      601 acccgggcga tataatgaaa attgtcgggc aagggtgacc gccgatgacg gtcagggtga
      661 gacgaacgag atggtctact accttccgaa ttcccaggag cctgttgctc tggtcagagt
      721 tcctgccggg gattttgcga tggggtcaac tgtttatacc gacgaacagc cggttcacac
      781 ggtctatctg gatgagtact ggatcatgaa gtatcccttg acggtgggtc agtggcgcgt
      841 gtatgcgcag gcaaatggct tggcgatgcc ttcggaaccg ggctggggtg gatcatatac
      901 gggttatatg actgacacca acttcaacaa gtatcctatt gtcaacgtat cctggaacga
      961 cgtgcaggca tttgcgctct ggtcgggtct caagttgccg actgaggcac agtgggagaa
     1021 ggcggcacgt ggaacggatt cgaggaggta tccctgggga gcggaggaac cggattcagg
     1081 tggtctctat cgtgccaatc tgagcggttc aggcgacggg tacgcataca cctctcctgt
     1141 tggaacatac ggactgggcg tgagccccta tggtgcttac gacatggcgg gcaatgtttg
     1201 ggagtggtgt gccgattggt acagcgcgac gtattactcg cagacaccgc cgggcggttg
     1261 ggtgaacccg ttgggtccga cgagcggctc ctaccgcgtg ctccgcgggg gcagttggaa
     1321 caacgatgcg ggcagcgcgc gttgctcggc tcgctacaac ggcacgccca cggttaggga
     1381 cgactac
//
LOCUS       DAOQUC010001269         1387 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_132796_length_1387_cov_2.150901, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001269 DAOQUC010000000
VERSION     DAOQUC010001269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1387
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132796_length_1387_cov_2.150901"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1387)
                     /locus_tag="PKH07_17475"
     CDS             complement(<1..>1387)
                     /locus_tag="PKH07_17475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF499 domain-containing protein"
                     /protein_id="HNT36783.1"
                     /translation="VRADDEKGTSPGDALRELFNEYAPCLILIDEWVAYARQLHERSD
                     LPAGSFETQFTFAQALSESAKLSKQCLLVISLPASDTAGSPHTQADDSEVGGQRGREA
                     LERLRNVIGRIESSWRPASAEESFEIVRRRLFEPLVDPDQFKARDVIARAFADLYNTQ
                     HEEFPPESREPDYEKRLRAAYPIHPEIFDRLYTDWSTLVKFQRTRGVLRLMAVVIHSL
                     WEKQDRNPLILPSNIPIDEPLVQFELTRYLSDNWVPVIEKDVDGANSLPLRLDGEIPN
                     LGKYAACRRVARTIYMGSAPTMAAANRGLEDRRVKLGCVVPGESPAVFGDALRRLASA
                     ATYLYQDGTRYWYSTQPTVTKVAEDRAEQLKREPEKVSHEIEGRLRENLRKCGDFSRV
                     HPLPQSTAEVPDDTDTRLVVLSVDQPYSKEPNSAAQTAAEAILQYRGSTPRIYRNTLV
                     FLALDKTRLQDL"
BASE COUNT          284 a          381 c          397 g          325 t
ORIGIN      
        1 acaggtcttg aagtcgcgtt ttgtccagtg cgagaaagac caatgtgttt cggtagattc
       61 ttggagtgct tccgcgatac tgcaggattg cttccgcagc agtctgagcg gcgctattgg
      121 gttctttgct atagggttga tccacgctca gaacaacaag ccgtgtgtct gtgtcatcgg
      181 gtacttccgc cgtagactgg ggcagcgggt gaacgcgaga gaaatcaccg cattttcgaa
      241 ggttctctcg gaggcgtccc tcgatttcgt gtgaaacttt ctccggttcc ctcttgagct
      301 gttccgcgcg gtcttcagca actttggtga ccgttggttg ggtcgagtac cagtatcttg
      361 tgccgtcctg atataggtat gtggctgcgc ttgccaggcg tctgagagcg tcaccgaaaa
      421 ctgcagggga ttccccgggc accacgcatc ccagtttgac ccgacggtct tccaaaccgc
      481 ggttggcggc tgccatcgtc ggggcggaac ccatgtaaat agttcgggcg acacgacgac
      541 aggcggcata ctttcccagg ttcggaatct caccgtccag tcgcaggggc aacgaattag
      601 ctccgtccac gtccttctcg atgacgggaa cccagttgtc cgacagatat cgggtcaact
      661 cgaactgcac cagcggttcg tcaatgggga tatttgaggg cagaatcagc gggtttcggt
      721 cttgcttctc ccaaagactg tgaatcacca ctgccatcaa acgcagcacg ccacgtgtgc
      781 gctgaaactt caccagcgta gaccagtcgg tatagagccg atcgaagatt tcgggatgga
      841 tgggatatgc cgcccggaga cgcttttcgt agtcgggctc gcgagattca ggtggaaatt
      901 cttcatgctg tgtgttgtac agatcggcaa acgctcgggc gatgacatca cgagccttga
      961 actgatctgg gtcaaccaaa ggttcaaaca gcctccgtcg gacgatttca aaactctctt
     1021 cagcgctggc tggacgccag gacgattcga tccgtccgat gacatttctc agccgttcca
     1081 gagcctctct gccacgctgt ccgcccactt ccgagtcgtc cgcctgtgtg tggggtgaac
     1141 ccgctgtgtc tgatgcgggg agactgatca ccagcaggca ttgcttggac agctttgccg
     1201 attcggaaag cgcctgggca aaggtgaact gcgtttcgaa actccccgca ggcagatcgc
     1261 tccgctcatg cagttgacga gcataagcga cccattcgtc aatgagaatg agacaagggg
     1321 cgtattcatt gaacaactct cgaagggcgt ccccagggct ggttcccttt tcatcatcgg
     1381 cacggac
//
LOCUS       DAOQUC010001270         1386 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_132883_length_1386_cov_3.114951, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001270 DAOQUC010000000
VERSION     DAOQUC010001270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1386)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1386)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1386
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132883_length_1386_cov_3.114951"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1005)
                     /gene="gcvT"
                     /locus_tag="PKH07_17480"
     CDS             complement(<1..1005)
                     /gene="gcvT"
                     /locus_tag="PKH07_17480"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HNT36784.1"
                     /translation="MSTNIRETVFSSRHQQLGGRMVDFGGWKLPVQFDGVLAEHLHCR
                     SAAVVFDTSHMGQFLISGREAASQLSLLCTQDAETLRVGQCRYGFLLREDAGIIDDTI
                     LMRTADDEFLLVVNAATQDRDQHWVEEHLRGNSSFVNRSALGWAKIDLQGPESFDVLR
                     GLVDIDLRTLPYFSVAPARICEVSCVLSRTGYTGELGYEIMAEGNDLLTVFDRILEHP
                     RVKPAGLGARDSLRLEMGYPLYGHELSEERNPVEAAMLFFLKSQKPHVGFEALNQARW
                     NGTAQTLVAFRTDTRRRPSPSCEICSDGEVVGVVTSGAFCPSLDVSAGMGFVRSDLAR
                     Q"
     gene            complement(1002..>1386)
                     /gene="rnk"
                     /locus_tag="PKH07_17485"
     CDS             complement(1002..>1386)
                     /gene="rnk"
                     /locus_tag="PKH07_17485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015838220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HNT36785.1"
                     /translation="DLARLTKLLQESEATAYRGSEYLAKLSAELARAKVVAAEEISNK
                     VITMHSQVCLEDLETGDRETYTLVFPEEADVDQRRISILAPIGTAMLGYEVGDVFEWE
                     VPAGKRNLQVKEILYQPEAAGDYHL"
BASE COUNT          323 a          421 c          329 g          313 t
ORIGIN      
        1 ctgtcttgcc aaatccgaac gcacaaaacc catgcctgca gacacgtcga ggctcggaca
       61 gaacgcacca ctggtcacca cgccaacaac ttccccatcg ctgcaaatct cacaacttgg
      121 actgggacgt ctacgagtat ccgttcgaaa cgccacaagg gtttgagccg tgccgttcca
      181 cctggcttgg ttcaaagcct cgaaaccaac gtgaggcttc tgactcttca ggaaaaagag
      241 cattgccgcc tcgaccggat tgcgctcttc cgagagctca tggccgtaaa gaggataacc
      301 catctcgagc cgaagtgaat ctcgcgcacc cagcccagca ggtttgactc gaggatgttc
      361 cagaattctg tcgaatacgg tcaacagatc gttgccttct gccatgatct catagcccag
      421 ttcacccgta taacccgttc tgctgagaac gcaggacacc tcgcaaattc gggctggcgc
      481 gacactgaaa tagggaagcg ttctgaggtc aatatcaacc aatcctctca gtacgtcaaa
      541 agactccggc ccctgaagat cgattttcgc ccatcccaac gcactgcgat tgacaaagga
      601 actgtttcct ctgagatgtt cttcaaccca gtgttggtct ctgtcctgag tcgccgcatt
      661 cacgacgagc aggaactcgt catctgctgt tctcatcagg atcgtgtcgt caatgatgcc
      721 agcatcttcc ctgagcaaga agccatagcg acactgaccc acgcgaaggg tctccgcatc
      781 ctgggtacag agcagtgaca gctgcgaggc ggcttctctg ccactgatga gaaactgccc
      841 catgtggcta gtgtcaaaaa caaccgccgc acttcgacag tgaagatgct cagcgagcac
      901 accgtcaaac tgcacgggca gtttccaccc cccgaaatcc accatgcgac caccaagttg
      961 ctgatgtctt gaactgaaga ctgtctctct gatgttcgtg ctcaaaggtg atagtctcct
     1021 gcggcctctg gctgataaag aatctctttg acttggaggt ttctcttccc tgccggtacc
     1081 tcccattcaa aaacgtctcc aacttcatac ccgagcatcg ccgtgccgat gggtgccaga
     1141 atggagattc gtctctgatc cacatccgct tcctcgggaa agacgagcgt gtaagtctcc
     1201 ctgtcgccag tctccagatc ttcaagacag acctgtgaat gcatggtgat gaccttattg
     1261 gatatctctt ccgcagcaac gaccttcgca cgcgccagtt ccgcgctcag tttggcgagg
     1321 tattcacttc cgcggtacgc agtggcctca gattcctgga gcagttttgt cagtcgagcc
     1381 aggtct
//
LOCUS       DAOQUC010001271         1386 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_132937_length_1386_cov_2.279489, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001271 DAOQUC010000000
VERSION     DAOQUC010001271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1386)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1386)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1386
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132937_length_1386_cov_2.279489"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1242
                     /locus_tag="PKH07_17490"
     CDS             <1..1242
                     /locus_tag="PKH07_17490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36786.1"
                     /translation="PFGGTALGGQLLLVNSSGSSPLFVAIPTFEGEGASEVLERLCSM
                     VNEKDPFGWFEGSHFSGAPPYLEPVDGGLSLTGFLGEYFLAGTELGLNIPKPPTSVSA
                     VYDPHSNCVSVMWDNPSGDKPYDGVSLIINGSVMTVPRPVSRYDIDLNVSRSDPSAFS
                     IWVVGLVRDASSGAGGILNEDGSGCLYVGIPSGAGGIMLRNNTQEEIADLPFVGGVAP
                     NWTDWGNGDVILEEGTKSTHYDDAIRRPPPRIKKPEDKTRFQMLRSDSSGAVGGAYRR
                     FLGLTPGHTYRLTMRLSTLKAAETGDCALSAHAIADSSKSALLSFAQMEGSESLPDGR
                     EGQKAARFALLNRQHLTNGVWTEVGTAVTPKDAELETRDITLPESGDCIFVWLRLAGS
                     CPTGVGMDWIKLEDISETQTP"
BASE COUNT          303 a          359 c          405 g          319 t
ORIGIN      
        1 ccctttggag gtacggcgct tggaggtcaa ctgcttctcg taaactcctc tggctcatcc
       61 cccctctttg tggcgatacc aactttcgag ggtgaggggg caagcgaggt actggaaaga
      121 ctctgctcga tggtgaacga gaaggatcct tttggatggt tcgaaggttc ccatttctcc
      181 ggagccccgc catatctcga acctgtagac gggggactca gcctgacagg cttccttggc
      241 gagtatttcc tcgcggggac tgagctgggg ctgaacattc cgaagccccc tacgtcggta
      301 agcgccgtct acgaccctca cagcaattgc gtctcggtga tgtgggacaa tccgtctgga
      361 gataagccct atgacggagt atctctgatc atcaacggct ctgtgatgac agtaccgcgt
      421 ccggtgtcaa gatatgacat tgatctgaac gtcagccgaa gcgatccgtc tgcattcagt
      481 atctgggtcg tcgggctggt cagagatgca tcgtcgggag ctggcgggat actcaacgag
      541 gatggttccg gctgtttgta tgtcgggatc ccttccggcg caggtggtat catgttgaga
      601 aacaatacgc aggaggagat cgctgatctg ccctttgttg gcggtgtcgc gcccaactgg
      661 actgattggg gcaatggaga cgtaattctg gaggagggaa ccaaatccac ccactacgac
      721 gatgcaattc gcaggcctcc accacgaata aagaagccag aagacaagac ccgatttcaa
      781 atgctgcgga gcgactcctc aggtgctgta gggggtgcct acagaaggtt tctgggattg
      841 acgccaggcc atacatacag acttaccatg cggttgagca ctctcaaagc cgccgagacg
      901 ggtgactgtg cattgtctgc acacgcgatc gctgactcct caaaatctgc tctgctctcg
      961 tttgcccaga tggaggggag tgaatctttg cccgacggtc gggaggggca gaaagccgct
     1021 cgatttgcat tgctcaatcg gcagcatttg acgaacggtg tctggactga agtgggaacg
     1081 gccgtaacac ccaaagacgc agaactggaa actcgggaca tcactctacc cgagagtggg
     1141 gactgcatct tcgtctggct tagacttgcc ggttcgtgtc cgacgggagt tggcatggat
     1201 tggatcaaac ttgaggacat ctccgagaca cagaccccat aactaagcgg cttcagtagc
     1261 aaggcgttac gcgcttatga agcagggcat gatagagatc ggccttcaat agcactgatg
     1321 tgtctgccga tccatcttgc gagatttcgg agcgtttccc gtgttcgttc tattctcctt
     1381 cgtgtc
//
LOCUS       DAOQUC010001272         1386 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_132945_length_1386_cov_2.208866, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001272 DAOQUC010000000
VERSION     DAOQUC010001272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1386)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1386)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1386
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132945_length_1386_cov_2.208866"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..783
                     /locus_tag="PKH07_17495"
     CDS             <1..783
                     /locus_tag="PKH07_17495"
                     /inference="COORDINATES: protein motif:HMM:NF015728.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RAMP superfamily CRISPR-associated protein"
                     /protein_id="HNT36787.1"
                     /translation="LGWLGGHSGQGNGWFHLKEISEAFVNSPETLLQWLEGGLKFEAI
                     NVAKEQNNHSKVSEYALTITLDGASEGYGREGLLVGSSDSVRWLSPPTYLDMQKADES
                     IDIPFARSLKWSCSAGKYTDSLFIPGSAIRGPLRAAAERIVGMCGFSENEINSLFGTT
                     EGGGRLKIRDAFTTEQTKAVPIFRHAEDEVLRSTCDGALFSEETIFDGSFTTRLVIID
                     PKQEDTKILEALGSLAECGFLPVGAHGGTPKISIEKRNGGGQ"
     gene            780..1322
                     /locus_tag="PKH07_17500"
     CDS             780..1322
                     /locus_tag="PKH07_17500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36788.1"
                     /translation="MMTETTVYTHSIPNATEKDVLDLAEKVFGSYPFDIMGELFWEYG
                     HYLGLMEKGKNGGGAEEVLPGSFWRQITSAKLQDYLTKASELFLFDSMSQIHILADGE
                     GNYRCVAVSEQVINGGEPCSLSKAEMELMPTSDNGKFSYAAKTGALQTPSGAEKLVAI
                     QYRKDNQVIMQRLVRKGGTS"
BASE COUNT          363 a          304 c          413 g          306 t
ORIGIN      
        1 ctcggctggc tgggggggca cagtggtcaa ggcaacggtt ggtttcatct taaggagatc
       61 tccgaggcgt tcgtgaactc gccggagacc cttctccaat ggcttgaagg cggactcaaa
      121 ttcgaagcga ttaatgtcgc caaggaacag aataaccatt cgaaggtttc tgaatacgcc
      181 cttacaataa cactggacgg tgcgagcgaa ggctacgggc gagaggggtt attggtcgga
      241 tcatcggatt cggttcgttg gctgagtccc cccacatacc tagacatgca gaaagcggat
      301 gagtctattg acattccgtt tgcccgaagc ctgaagtgga gttgcagcgc aggcaaatac
      361 acggacagtc tgttcattcc tggttctgca attcgaggtc ctctcagggc tgccgcggaa
      421 aggatagtag ggatgtgcgg cttttcggaa aatgaaatca actctctctt tggtacgacg
      481 gaaggtggcg ggcgactcaa gataagagac gcgtttacca cagagcaaac caaggcagtt
      541 cctatcttcc gccatgcgga ggatgaggtt ctgcgcagca catgcgacgg agcgttgttc
      601 agtgaagaga cgatcttcga tggctcgttc acgacgcgcc tcgtaataat tgatcctaag
      661 caagaggaca ccaagattct tgaagcgctt ggaagtcttg ccgagtgtgg gtttcttccg
      721 gtaggtgctc atggtggcac gccgaagatc agcatagaga aaagaaatgg agggggacaa
      781 tgatgacaga gacaaccgtc tatacccata gcattccaaa tgccactgag aaggacgtgc
      841 tggatcttgc tgagaaggtg tttggttcat atccgtttga cattatgggt gagttgttct
      901 gggaatatgg gcactacctt ggtctgatgg agaaagggaa gaatgggggc ggggcagaag
      961 aggtcttgcc tggctccttt tggagacaga tcacttcagc taagttgcag gattacttga
     1021 caaaggcaag tgagttattc ctgtttgact ccatgtccca gatccatatt cttgccgacg
     1081 gtgagggcaa ctatcgttgt gtggcagttt cggaacaggt aattaacgga ggggagccct
     1141 gttccttgtc caaagccgaa atggaactga tgccaacaag tgacaacggt aagttctctt
     1201 acgcagccaa aacgggtgcg cttcaaacac ccagtggtgc cgagaaactc gtagcgatcc
     1261 agtataggaa agacaaccaa gtcatcatgc agaggctggt gcgaaaggga ggaacgtcat
     1321 gagaaataac aactatggcg acaggcgggg aacgcacact caaggtcgcg gacaaggcgg
     1381 aagaag
//
LOCUS       DAOQUC010001273         1384 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_133210_length_1384_cov_3.224229, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001273 DAOQUC010000000
VERSION     DAOQUC010001273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1384
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_133210_length_1384_cov_3.224229"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="PKH07_17505"
     CDS             complement(<1..181)
                     /locus_tag="PKH07_17505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002617664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HNT36789.1"
                     /translation="MIATRVPNFEHARQQMIDFQLRSRGIRSERVLGAMGRIPREEFL
                     PDSVRSEAYEDHPIPI"
     gene            complement(206..1030)
                     /gene="surE"
                     /locus_tag="PKH07_17510"
     CDS             complement(206..1030)
                     /gene="surE"
                     /locus_tag="PKH07_17510"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012648406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HNT36790.1"
                     /translation="MNRTVLLKMILLTNDDGIHARGLLTLAKTLREIDRLVIIAPDRE
                     RSAVAHSFTMNHPIRAHRLEEDVIMIDGTPTDCVMLGVLGYLAEKPTIVVAGINSGAN
                     MGDDVTYSGTVAAALEGTLLGLPSIAVSLENRMHRAHSPAEKTDLHYETAACFAGQLV
                     KRVMQTGLPKGILLNANVPNLPHDQIAGVRVTRLGKRIYNDRIERRVDPQGREYFWLA
                     GEIPGYHAEEGTDFAAVEANCISVTPLRFEMTSLDSIPQLQDQGYQDGWTFVISST"
BASE COUNT          298 a          371 c          392 g          323 t
ORIGIN      
        1 caatggggat cggatggtct tcataggctt ccgaccggac agagtcaggc aggaattcct
       61 cgcgcgggat gcgtcccatc gctcccagaa ctctctcgga acgaattccg cgagagcgca
      121 gctggaagtc aatcatctgc tgtcgggcat gttcgaaatt cggtactctc gtggcgatca
      181 tctccggcgc tcttgagaac ggccgttatg tggatgaaat cacaaacgtc cagccgtctt
      241 gatacccctg gtcttgaagt tgcgggatgg agtccagcga ggtcatctcg aatctcagcg
      301 gcgtgacgga aatgcagttg gcctctacgg cggcaaagtc tgttccctct tctgcgtggt
      361 atcctggaat ttcgcccgcg agccagaagt actcccgtcc ttgaggatca actcggcgct
      421 cgatacgatc attgtaaatg cgcttgccga gtcgtgtgac ccgaactcca gcgatttgat
      481 cgtgaggcag attgggaaca ttggcattga gtaaaatgcc cttgggaagt cccgtctgca
      541 tcacgcgctt taccaactga cctgcaaaac acgcagcggt ctcgtagtgc agatccgtct
      601 tctcagcagg ggagtgtgcg cggtgcatcc tattctccag ggatacggcg atcgaaggca
      661 agcccaggag cgttccttcc aaggcggctg ccacggtgcc tgaataagtg acatcatcgc
      721 ccatattggc tcctgagttg atgccggcca caacaatggt gggtttctcc gccagatagc
      781 cgaggacgcc aagcatcacg cagtcggttg gtgtgccgtc aatcatgatc acgtcttcct
      841 caagccgatg cgcccgaatt gggtggttca tcgtgaaaga gtgcgcaacc gcgctgcgtt
      901 cgcggtctgg agcgatgatg accagtcggt caatctcccg caacgttttg gccaacgtca
      961 gcagtccacg cgcatgaatg ccatcgtcat tggtcaacag gatcatcttc agcagcaccg
     1021 tccgattcat ctgattcaaa cacgggtatc ctaacctaac tccagcagtt tggtgtggat
     1081 ttttttgcag agacagcgat cgctctggcc tcgccctttt ttctctggac gatgtgtcat
     1141 cagtcagatt atgttatgga aacgacttga gggagaatca gtcaaggatt ctgcggattg
     1201 ggattggaat ctggcgtcca ccccaaccgt atgtggctcc tgtacaagga gtccgcttca
     1261 agcgcgtgag gagaggttgt atccatgaat tggcagaaga gaacccactg cggccaggtg
     1321 ggagaacctg atattggccg aaccatcgag ttgaccggtt gggtggatgc ccttcgagac
     1381 catg
//
LOCUS       DAOQUC010001274         1384 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_133238_length_1384_cov_2.699022, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001274 DAOQUC010000000
VERSION     DAOQUC010001274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1384
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_133238_length_1384_cov_2.699022"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="PKH07_17515"
     CDS             complement(<1..183)
                     /locus_tag="PKH07_17515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36791.1"
                     /translation="MRRFLTYLCVGCLLVTAIWVCEGNGELLEWIVAMVDGYPWWRIF
                     WRILLPVARPAIVAGTV"
     gene            complement(180..1349)
                     /locus_tag="PKH07_17520"
     CDS             complement(180..1349)
                     /locus_tag="PKH07_17520"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNT36792.1"
                     /translation="MTAESATYVTGSGGAIVRLLRRSKRTQTSDVTVSHFVENYPRPV
                     DLDDREGRNSELTAKQGYPLMETWSSEDGTRVPCHTLCVPVEIQGRVQGTVSVFRVGS
                     KESAYTSEERRLLGTLLSYAATCIENGRLFETVRRTERELRDAHQQLFLREKLAALGE
                     MAAGVAHELRNPLVSIGGYARRICKYVNNPDKVQECVSIITREVERLETLASDILKYV
                     SPREPSFAAVDLNVLFRDVFQMQREFANRKPVIHFQRDVSPEATYVWADREHLETALI
                     DLVRNAVEACVSGGSVIVRSEPHENDPTRIYIIVEDTGTGIPEETLQNIFTPFFTTKT
                     TGSGLGLATTKNVIDAHEGQIDVESRVGEGTVFRIALFRPPQQTDQQDNNGGSLQ"
BASE COUNT          317 a          414 c          339 g          314 t
ORIGIN      
        1 caccgttccg gcgacgatcg ccggccgtgc gacgggcagg agaatccgcc agaagattcg
       61 ccaccaggga tagccatcca ccatggcaac gatccactcc agcagttcgc cattgccttc
      121 acaaacccag atcgctgtaa ccagcaaaca tccaacgcac aggtaagtca ggaatctcct
      181 cattgtagtg aacctccatt gttgtcttgt tgatcggttt gttgcggggg tctgaaaagc
      241 gcaatccgaa acacggtccc ctcccccaca cgactctcca catcaatctg tccttcatgg
      301 gcatctatga cattctttgt ggttgccaga cccagaccgc taccagtcgt cttggttgtg
      361 aagaacggtg tgaagatgtt ttgaagcgtc tcttcgggaa tacccgttcc agtgtcttcg
      421 acgataatat agattcgtgt tggatcgttc tcgtgaggct cggaacggac tatgacagaa
      481 cctccggata cacatgcctc gacggcattt cgcaccaagt caattaacgc agtctcaaga
      541 tgctctcgat ccgcccagac gtatgtggcc tccgggctga catcccgttg aaagtgaatc
      601 accggcttcc gattggcaaa ttcacgctgc atctgaaata cgtctcgaaa caggacgttc
      661 aggtcaaccg cagcaaaaga aggctccctt ggcgaaacgt atttcagaat gtcgctggca
      721 agagtctcca aacgctccac ttctcgggtg atgatactga cgcactcctg caccttgtct
      781 ggattgttga catacttgca gattcggcgt gcgtatcctc ctatggacac cagtggattc
      841 cgtaattcgt gagctacgcc cgcggccatc tcacccaacg ccgcaagttt ctcacgaaga
      901 aacaactgct gatgggcatc gcgcagttcc ctctcagttc gcctcacggt ttcaaacagt
      961 cttccatttt cgatgcaggt tgcagcatac gagagaagcg ttccgagaag cctccgttcc
     1021 tccgaggtat atgctgactc tttggaacct actcggaaga cgctcacagt cccctgaacg
     1081 cgaccctgaa tctctacggg aacgcaaagc gtatgacagg ggactcgggt gccgtcctct
     1141 gaagaccacg tctccatcaa agggtagccc tgcttggcgg tcagttcgga gttccttcct
     1201 tcccgatcat ccagatcaac cggtctcggg tagttctcaa caaaatgaga gacagtcaca
     1261 tccgatgtct gagtgcgttt gcttcgtctg agaagccgaa caatcgcccc gcccgaaccg
     1321 gtcacatagg tcgcgctctc ggcggtcacc tacgcgaacg aggccaagac cgcggtcctc
     1381 ggcg
//
LOCUS       DAOQUC010001275         1383 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_133380_length_1383_cov_2.970633, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001275 DAOQUC010000000
VERSION     DAOQUC010001275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1383
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_133380_length_1383_cov_2.970633"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1383)
                     /locus_tag="PKH07_17525"
     CDS             complement(<1..>1383)
                     /locus_tag="PKH07_17525"
                     /inference="COORDINATES: protein motif:HMM:NF022112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HNT36793.1"
                     /translation="EGNPYAVERESIRSPQDALQALIAGPTPQEASLGLCTAFPSGTT
                     ILNLGVLQNGLHADFSKEILEGMDEVQVEWAFRQIYWTAAQFGLNESVRMSINGDDIS
                     KLLERRFSQESPSVDSLLEKTPLAGVGAGGIFRKRVMLSALGGYFWDGSDWRLQCAVV
                     EGHEPSDFVGVRLAERVKVYLESEGTDLSVARDMHVRQRVEGISGKPRWQEASYIWLS
                     QNGFPCIVYANSTGLCDTAQGATSRRNDDIRCAALAGQYDPNGATDITVLLSVQDLLG
                     DPHVVEPAPRVATFYGAQSASGYDQGSMRSRLLADAIQRSLTSSMAGAGFQWASSVPS
                     RGQGSFVGQVRISEQPSALVELSGSAADRIRDPFFQSCCAWAIYSGVCRYYGAVPRRE
                     MFSAEVVSHTFPETMIAGEARNAKVIYRNKSVLWDGEHGFALVAVDGSDPFAAPLHRI
                     PKGVTVETEE"
BASE COUNT          354 a          428 c          318 g          283 t
ORIGIN      
        1 tttcttcagt ttcaacagtc acgccctttg gaatgcgatg cagaggtgcg gcaaaaggat
       61 ccgaaccgtc cacggcaaca agtgcgaatc catgttctcc atcccagagt acactcttgt
      121 tgcggtagat taccttggca ttcctggctt cacctgcgat catcgtctcc ggaaaggtgt
      181 gagaaaccac ttcagcagaa aacatctctc gcctcggaac tgccccgtag taccgacaca
      241 caccgctgta aatagcccac gcacaacaag actgaaaaaa cggatcccga attcgatccg
      301 cggcagaacc actcaactcg accagagcgg aaggctgttc agaaatcctc acctgtccaa
      361 caaatgaacc ctgtccccga cttgggactg aagatgccca ctggaatcct gctccggcca
      421 tggatgatgt caacgaccgt tgaatcgcgt cggcaagaag tctgcttcgc atgcttccct
      481 gatcatatcc cgacgcagac tgagcaccgt aaaacgttgc gactcgcggc gcaggctcca
      541 caacatgcgg atcacccaga agatcctgga cgctcagaag gacagtgata tccgtcgctc
      601 cgttcggatc atactgcccc gccaatgcag cacaacgtat gtcatcgttg cggcgactcg
      661 ttgctccctg agccgtatca caaagacccg tggaattcgc ataaacgatg caggggaaac
      721 cgttctgaga aagccagatg taactcgctt cctgccatcg tggcttgccg gaaatgccct
      781 caacacgctg cctgacgtgc atatcccggg cgacactcag atcagttccc tcggactcga
      841 gatacacctt cacgcgctca gccagtctga cgccgacaaa gtctgaaggt tcatgtcctt
      901 ccacaacagc acattgcagt ctccaatccg agccgtccca gaagtacccg cctagagcag
      961 aaagcatgac acgtttgcga aaaatgcctc cagcgccaac tccggcaaga ggtgtcttct
     1021 caagcaaaga atccaccgat ggggattcct gagagaatct tcgttccagc agtttcgaga
     1081 tgtcgtcccc attgatcgac attcgcacag attcgttcaa cccaaactgc gctgcagtcc
     1141 agtatatctg tctgaatgcc cattcaactt gaacctcatc catcccctcc aaaatctctt
     1201 tcgaaaagtc tgcatgaagg ccattctgaa gaaccccaag attcagtatc gtggttcctg
     1261 aaggaaaagc ggtgcacaaa cccagagaag cttcctgggg cgttggcccc gcaatcaatg
     1321 cctgaagggc gtcctgaggc gaccgaatag actcacgctc cacggcgtac ggattgccct
     1381 ccc
//
LOCUS       DAOQUC010001276         1382 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_133558_length_1382_cov_2.628485, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001276 DAOQUC010000000
VERSION     DAOQUC010001276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1382)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1382)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1382
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_133558_length_1382_cov_2.628485"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            17..>1382
                     /locus_tag="PKH07_17530"
     CDS             17..>1382
                     /locus_tag="PKH07_17530"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNT36794.1"
                     /translation="MARHLLGLFVICALLTGGAAFGEEGVYVLDGRGGLYSAGTVSGM
                     PEGPYFEWNIARDLVLAPNGEGYYVLTGDGQIFEVGQVPDLDGQFFGWDIVRQLILAP
                     QGHYVLDGFGILHAFDGAPKLESTVLPEPVAQAFALAPDGRGGYILDRFGGIHPVGDV
                     EPVVGPYFGWDIARDLVLNEDGSFYVLDGYGGIHCEGSVFLNGPYFTFDTAKRLAVLP
                     TGDGAMVLDGQGGIHPVGNAPAIMADFSLRGNSAVDIELSSSGIQLGAIQGQVETDAP
                     APGGQMAPMPPAILQGLIARLEGTDFATNVAPDGFFKFNGIPEGVYALLIENPANPDL
                     KLRMARIRVRRGMTWDLGRICLRQTGRIIGTVQLEGEQMHMGTDVLLAGSSLLAKTDR
                     DGSFRLVFVPEGDYTVVIEHPGFVPVEVTDVQVSVGSVTDLGTILLRRLDPSLLGAVE
                     GVVLK"
BASE COUNT          278 a          351 c          415 g          338 t
ORIGIN      
        1 atttccaatg aaaaacatgg cgaggcatct gttgggtctt tttgtcatct gtgcactgtt
       61 gacggggggt gcagcctttg gtgaagaggg cgtctatgta ttggacggcc gtggaggact
      121 gtattcggcg ggaacggtca gtggcatgcc agagggcccc tactttgaat ggaacatcgc
      181 tcgggatcta gttctcgctc ccaatgggga gggatactat gtattgactg gagacggtca
      241 gatatttgaa gtgggtcagg ttcccgacct cgacggacag ttcttcggat gggatatcgt
      301 acgccaactg attcttgcac ctcagggtca ttatgttctg gatggctttg ggatcttaca
      361 tgctttcgat ggggctccca aattggaaag caccgtattg cccgagccgg tggcgcaggc
      421 tttcgcgctg gctccggatg gccgcggtgg ctacatactc gatcgctttg gtggaattca
      481 ccccgttgga gacgtggaac ccgttgtcgg gccctacttt ggatgggaca ttgcgcgtga
      541 cctcgtgttg aacgaggacg gttccttcta cgtgctggac gggtatggtg gaattcattg
      601 cgaaggctcc gttttcctga atggcccgta tttcacgttt gacacagcaa aacgtctcgc
      661 ggtacttcca accggagacg gcgcgatggt gctcgatgga caaggcggaa ttcacccagt
      721 gggaaatgca cctgccatca tggcagactt cagccttcgg ggaaactcgg cagtagacat
      781 tgaactcagt tcttctggca tccagctggg tgccattcaa ggtcaagtcg agacagacgc
      841 gccggcccct ggcggccaga tggcaccgat gccgcctgcc attcttcagg gactcatcgc
      901 ccgccttgag ggaaccgatt ttgcgacaaa tgtcgcgccg gatggtttct tcaagttcaa
      961 tggaattccc gaaggggtct acgcactact tatcgaaaat cccgccaatc cagatttgaa
     1021 actcaggatg gcacgcatcc gtgttcgtcg tggcatgacc tgggatctgg gtcgtatctg
     1081 cctgcgtcag accggtcgaa tcatcggaac ggtccaactg gaaggcgagc agatgcacat
     1141 gggaacggac gtcctccttg cggggtcatc gctcctggcg aaaacagatc gagatggatc
     1201 gttccgactc gtcttcgtgc ctgaggggga ttacaccgtt gtcatagagc atccagggtt
     1261 tgttcccgtt gaggtgacgg atgtccaagt cagcgtcggt tccgtgaccg atctcggaac
     1321 cattcttctg agacgcctcg atccaagtct tctcggtgca gtcgaaggag ttgttctcaa
     1381 ga
//
LOCUS       DAOQUC010001277         1378 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_134154_length_1378_cov_4.472411, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001277 DAOQUC010000000
VERSION     DAOQUC010001277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1378
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_134154_length_1378_cov_4.472411"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..840
                     /locus_tag="PKH07_17535"
     CDS             <1..840
                     /locus_tag="PKH07_17535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36795.1"
                     /translation="VEKPGYDYRYYPNEDRFSYRKGFIPIHVPDESNPVMFKIRKRAT
                     KPTFLLYSPYVRLDFNDSSGNEYGHDVLRADMGNSEMRDGWFDDFRVVANRTESATEA
                     VLVFSAGGEDAGFVASSEFLGMAPESGYSGEYVLCVKDGVDPQERYLYLRSRQPLLYS
                     VAELHGVRVGRRGVFVQIRLRTNPLGDRNLEMDEQFPIAARHHIQAKVREAYRDGVPL
                     DVTPLKELIEVARGTTEAESHRIADQVDELVKKVEREERRNFEIDTATLTEMYRKAKK
                     IKD"
     gene            846..1103
                     /locus_tag="PKH07_17540"
     CDS             846..1103
                     /locus_tag="PKH07_17540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36796.1"
                     /translation="MRHSCREKEREHLVPDRIRVGRDAPTPVPGAFFTSLRAASRRNT
                     GIRADDPLPWDRRASARQSGERTRAKWTLGVPRRASRRRLG"
BASE COUNT          350 a          326 c          405 g          297 t
ORIGIN      
        1 gtggaaaaac cgggttacga ctatcgttac tatccaaatg aagatcgatt cagctaccgc
       61 aagggcttca tacccattca tgttcctgat gagtcaaatc ctgtgatgtt caagatacgc
      121 aagagggcta ccaaaccaac gttcctcttg tatagcccct acgtgagact cgatttcaac
      181 gacagcagtg gtaacgaata cggccatgat gtgttacggg cagatatggg taacagcgaa
      241 atgcgcgacg gctggttcga tgactttcgt gtggttgcca accgaacaga atctgcaacc
      301 gaggctgttc ttgtttttag tgctggcggg gaagatgctg gatttgttgc atcgagtgag
      361 tttctgggaa tggctccaga atcaggatat tcaggagagt atgtcctttg tgtcaaagac
      421 ggtgtagatc cgcaggaaag atatctctac ctaaggagtc gtcagcctct gctctatagt
      481 gtggccgaac ttcacggtgt gcgcgtgggc agaagaggtg tctttgtcca gatccggctt
      541 cgcaccaatc ctctcggcga tcgcaatctg gagatggacg agcagtttcc cattgcagct
      601 cggcaccata tccaggcaaa agtaagagaa gcctaccgtg atggtgttcc ccttgacgtt
      661 acacctctca aggagctgat cgaggtggcg agaggaacaa ccgaagccga gagtcatcgg
      721 atcgcggatc aggtggatga actcgtcaag aaggtcgagc gcgaagagag aagaaacttc
      781 gaaattgata cggcaacatt gactgaaatg taccgcaagg cgaagaagat caaggattga
      841 gagcagtgcg gcattcatgc cgcgaaaagg agcgggagca tcttgttccc gacagaatcc
      901 gtgtggggcg agacgcccca actcctgtgc ctggtgcctt cttcacctcg ttgagggctg
      961 cttctcgacg taacacaggc attcgtgccg atgatcctct gccctgggat cgccgagcgt
     1021 ctgctcggca gtccggagaa agaacaagag ccaagtggac acttggcgtt cccaggagag
     1081 cttctcgaag aaggctggga tgagggcaac ggagcgggag catcttgctc ccgacagaat
     1141 ccgtgtgggg cgagacgccc cacctccaga agaatttgac cgggcgtttc gaatatgaga
     1201 gacttggcat catgatgagt tcatggattc aagataaatc agcagccctc agaaccgctt
     1261 cgcgaacctg aaggctggca cccagaggag tcagtcggag tatgagaaga gggagggtac
     1321 ccggcagcga agagtctctc tgtgcctttg cagacaggat tcggactgta agagagat
//
LOCUS       DAOQUC010001278         1378 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_134162_length_1378_cov_3.947090, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001278 DAOQUC010000000
VERSION     DAOQUC010001278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1378
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_134162_length_1378_cov_3.947090"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            92..1285
                     /locus_tag="PKH07_17545"
     CDS             92..1285
                     /locus_tag="PKH07_17545"
                     /inference="COORDINATES: protein motif:HMM:NF015418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HNT36797.1"
                     /translation="MIPVAEALNILDSTLAPLKRSSERVILANSLGRVLSEDQISTLD
                     LPPFNKSAMDGYAIGIGDDRDAYRVLGMIPAGSAPRERFAAGTCFKVMTGAPIPEGTG
                     KVIMVEDTIEEQSTVRILRRRDQSNICEKGEDVSAGDRILPAGTRLDAADIANLVACG
                     ICSAKCVPRPKVAVLSTGNELVSSVDNIEPGKIIDTNGPLLESLALTFGMNVTRRGLV
                     DDDAESTARWIESALDISDIVLASGGVSEGDFDTVCPALQALGATIHFSRVAMKPGKP
                     TVFASLGEKVIFALPGNPVSVHLTFHLFVLRAVSILTSSVPLFRSFVLAMQCDWRRRN
                     ADRAQFVPCRLTHDGRIEPVVFHGSAHLAAISVTDGYAHVPAGVEEVREGEKVEFLAV
                     KGLCK"
     gene            1282..>1378
                     /locus_tag="PKH07_17550"
     CDS             1282..>1378
                     /locus_tag="PKH07_17550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HNT36798.1"
                     /translation="MKDRYGRPITYLRISITDRCNLRCIYCMPAEG"
BASE COUNT          322 a          332 c          392 g          332 t
ORIGIN      
        1 agtcgtatag aaagacgacc cagagccttt gcgacttggc acaaggtcgc tttgaccagc
       61 tgtggaaaga gcaacaaagg acaacccaga gatgatacct gttgccgaag ctttgaacat
      121 cctcgacagc acattggctc cgctgaagag atcctctgag cgtgtcattc tcgccaactc
      181 cttaggccgt gtgctttcag aggatcagat ttcaactctt gacctgcctc cattcaataa
      241 gtcagccatg gacggctatg ctattgggat aggagatgac agagacgcat acagagtcct
      301 tgggatgatt cccgcaggaa gtgcaccgcg agagcgcttc gccgcgggga cctgtttcaa
      361 ggtgatgacc ggtgctccca ttccagaggg aacgggcaag gttatcatgg ttgaggatac
      421 catcgaggaa cagagtactg tcagaattct tcgacgtcga gatcagagca atatctgtga
      481 gaagggggaa gacgtatctg caggagacag gattcttccg gctggaacga gattggacgc
      541 agcagatatt gcgaatctag ttgcatgtgg catctgttct gcaaagtgtg tgccccgacc
      601 aaaggtcgcg gttctgagca cagggaacga actggtcagt tctgtggaca acatagaacc
      661 cggcaagatc atagacacca acggcccctt gctcgaaagc cttgctctaa ctttcggcat
      721 gaatgtcact cggcgcggtc ttgtggatga tgacgccgag tcaacagcac gatggatcga
      781 atctgctctc gacatcagtg atatagtcct ggcctcaggc ggtgtctccg agggggactt
      841 cgacacagtt tgtcctgcac tccaagcatt gggagctacg atccatttta gtcgagtcgc
      901 catgaaacca ggcaagccga cagtattcgc ctctctgggg gagaaggtca tctttgctct
      961 ccctgggaat cccgtatcag ttcatctgac atttcacctt tttgtgttgc gggcagtctc
     1021 gattctcacg agttctgtgc ctttgttcag aagtttcgtc ctggcgatgc agtgtgactg
     1081 gaggcggaga aatgcagaca gagcacagtt tgttccgtgt cgtcttactc acgatggaag
     1141 gattgagcct gttgttttcc atggatctgc gcaccttgcc gcgattagtg tgacagatgg
     1201 atatgcccat gttcctgcag gagttgagga agtcagggaa ggggagaagg tggagttcct
     1261 tgctgtgaag ggtttgtgca aatgaaggat cgttacggtc gcccgatcac ctatcttagg
     1321 atttcgatca ccgacaggtg taacctgcgc tgcatctact gtatgcccgc agagggcg
//
LOCUS       DAOQUC010001279         1378 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_134188_length_1378_cov_3.229781, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001279 DAOQUC010000000
VERSION     DAOQUC010001279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1378
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_134188_length_1378_cov_3.229781"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(91..>1378)
                     /locus_tag="PKH07_17555"
     CDS             complement(91..>1378)
                     /locus_tag="PKH07_17555"
                     /inference="COORDINATES: protein motif:HMM:NF021570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2298 domain-containing protein"
                     /protein_id="HNT36799.1"
                     /translation="LAGFVLVLKEIIHARSEAGTTWTQWLLAGLGRGVAFAVAVVGLA
                     YILYLPFFLKYSPPVQGAEGMLKTTLFRTDLSEYVFVFGTFLFLILAYLVAQLLLTIK
                     AHETTIGKVAFMVLVMLFVLGWGFEGYVLVPLMISLLLAFSYLLIRDLKDSPARVFAL
                     ALVLMALGIKVACERVYIVDGYGNDLQRMNTLFKLHLQAWVVLAVACPFLLSEIIRNR
                     SLALGARVGTVIALVVLCLVGFITTQSMARQVFDPADALEKQFSSLDIKTLDGQQYLK
                     FVRPDTVQRWPSARGPEGSDEYRAIQWLINNVQGQPVIAEATGDPYRYYGRVSANTGL
                     PTILGWFNHESVWRKDADGKLYEHLNERKADVEKIYKSTDIAEAAAILKKYNASYLYL
                     GPLEREAYPGKGLDKFQQAGWASAFSSQEVNIYRVP"
BASE COUNT          341 a          398 c          366 g          273 t
ORIGIN      
        1 cgcagtcctc tggaatggta gcagcgggca cagagcggaa acaactccgg ctccctcgcc
       61 cctttgtggg agtgggttgg ggtgaggggg ctaaggaact cgatagatgt tcacttcttg
      121 gcttgagaag gcgctcgccc atccggcctg ttgaaactta tccagtccct ttcccggata
      181 ggcttccctt tcgagaggac cgagatagag gtaactggca ttgtacttct tgagaatggc
      241 cgcggcctca gcaatgtccg tggacttgta aatcttctcc acatccgcct ttcgttcatt
      301 caggtgttcg taaagcttac cgtccgcgtc ttttctccag acggattcgt ggttgaacca
      361 gccgagaatg gtcggaagtc cggtgttggc agaaacgcga ccgtagtagc ggtagggatc
      421 gcccgttgcc tcagcaatga ccggctgtcc ttggacgttg ttgatgagcc attggatggc
      481 tcggtactcg tcagagcctt ccggtccccg cgcggatggc caacgttgca cggtatccgg
      541 cctgacaaac ttcaggtact gctgaccgtc cagggtcttg atgtccagcg atgagaactg
      601 cttctcaaga gcgtccgcgg gatcgaacac ctgtcgagcc atagactgcg ttgtgatgaa
      661 gccgaccagg cacagtacca ccagagcgat gaccgttcct actctggctc ccaacgcaag
      721 actccggttg cggatgatct cggaaagcag gaagggacag gcaaccgcca gcaccaccca
      781 cgcctgcaag tgcagtttga aaagcgtgtt catcctctgc agatcatttc catagccgtc
      841 cacaatgtag acacgctcac aggcgacctt gatccccagc gccatcagaa caagcgccag
      901 ggcaaacacc cgcgccggag agtccttgag atctcggatc aacaaatacg aaaacgcaag
      961 caacagagat atcatcagag gcacgagaac gtatccttca aaaccccacc ccagaacaaa
     1021 cagcatcacc aacaccatga aggcgacttt gccgatcgtc gtctcatgcg ccttgatcgt
     1081 gagaagcagt tgggcgacaa gataagccaa aatgagaaag agaaacgtcc caaagacgaa
     1141 gacatactcc gacaggtcag ttcgaaagag tgtcgtcttc agcattccct cggcgccctg
     1201 aaccggcggg ctgtatttca ggaagaaagg caggtagaga atatatgcca gaccaacaac
     1261 agcaaccgca aacgccactc cccttccaag gccggccagg agccattgcg tccacgtcgt
     1321 acctgcttct gatcgggcgt ggatgatctc tttcagcaca agaacaaaac cggccaac
//
LOCUS       DAOQUC010001280         1376 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_134528_length_1376_cov_2.968963, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001280 DAOQUC010000000
VERSION     DAOQUC010001280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1376
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_134528_length_1376_cov_2.968963"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..512)
                     /locus_tag="PKH07_17560"
     CDS             complement(<1..512)
                     /locus_tag="PKH07_17560"
                     /inference="COORDINATES: protein motif:HMM:NF040248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNT36800.1"
                     /translation="MVTYILKRIVLMIPTLILISIISFTIIQLPPGDYLTTYIANLAQ
                     TGEDADEALVASLRARYGLDQPLPMQYLKWMSGVLRGDFGQSFSMNKPVRELIWERLG
                     LTFIIALLSILLTYMIAIPIGIYSATHQYKASDYGITILGFIGMATPQFSLALLVMYF
                     SSEVLGVSVGG"
     gene            777..1211
                     /locus_tag="PKH07_17565"
     CDS             777..1211
                     /locus_tag="PKH07_17565"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNT36801.1"
                     /translation="MREKPLVLIADDSRSIVNIVSVVLKARNIDTLEAYDGRQAWNLF
                     EEHEPDLAVLDIVMPGRTGLELCSDIRSHPRLSHIPVLIMTSITKDSDLADGFWKLGT
                     SANDFVTKPFDPFDIADRIERLLRKAQQISDRKSSDSEAPKG"
     gene            1285..>1376
                     /locus_tag="PKH07_17570"
     CDS             1285..>1376
                     /locus_tag="PKH07_17570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011438813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HNT36802.1"
                     /translation="MNLLQAAELGAIQGLTEFLPVSSSGHLVIL"
BASE COUNT          345 a          332 c          373 g          326 t
ORIGIN      
        1 cccccaacgc ttacccccag cacttcggac gagaaataca tcacgagaag ggccagggaa
       61 aactgaggcg tggccattcc gatgaatccc aggatggtaa ttccataatc ggaggctttg
      121 tactgatgcg ttgccgagta gattccgatg ggaatggcga tcatgtaggt gagcagtatc
      181 gaaagcagcg cgatgatgaa tgtcagcccg agccgctccc agatcagctc ccgaacgggc
      241 ttgttcatgg agaacgactg accaaagtct cctctgagaa caccgctcat ccacttcaga
      301 tactgcatcg gcagaggctg atcaagaccg taacgtgctc tgaggcttgc cacgagcgct
      361 tcgtctgcat cctcaccggt ttgggcaaga ttggcgatgt aggtggtcaa gtaatcccct
      421 ggggggagtt gaattatggt gaatgagatg atggagatca ggatgagagt tgggatcatg
      481 agaacgatgc gtttcaaaat gtaggtaacc aattccgaag ccttccaatc ctgtcaggtc
      541 tgtgctcatg cagccaataa gcacaggctg aacgcattct catggatcat gctgattcct
      601 caaggcgagg ataacatacc atctgtttgg gacatggaca agtctcgaca gacgtcccgt
      661 tcagtgagtg atatactcgg cggcagaaat atcttgcggt agcatcgggt tttggggtaa
      721 atagagagca gtaaatacgc tcgaaagcag gagaccgaag gctgcaccag gacataatga
      781 gggaaaaacc gctcgttctt attgctgatg attcgcgtag cattgtcaat atcgtttctg
      841 ttgtgttgaa ggcgcgtaac attgacactc ttgaagcgta cgacggacga caggcgtgga
      901 atctctttga ggaacatgag cccgacctgg ccgttctgga cattgttatg cctggcagaa
      961 cggggcttga actgtgttcg gacatccgat ctcacccccg tctgagtcac attcctgtgc
     1021 tgatcatgac ttccatcacc aaggattcgg atttggccga tggattctgg aaactgggga
     1081 cttccgcaaa cgactttgtc acaaagccgt ttgatccctt tgacattgcc gaccgcattg
     1141 agcgcctgct cagaaaggca caacaaatct cggacagaaa gtcctctgat tcagaggcac
     1201 cgaaaggctg aatttgggta ggatcgccca tttgggactc caaaggttct ctttgtctgc
     1261 ccccggatcc aaagaggtta agatatgaat ctgttacagg cggctgaact tggtgcaatt
     1321 cagggactta ctgagtttct gcccgtgtcc agctcgggac accttgtcat cttgca
//
LOCUS       DAOQUC010001281         1375 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_134716_length_1375_cov_2.702273, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001281 DAOQUC010000000
VERSION     DAOQUC010001281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1375)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1375)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1375
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_134716_length_1375_cov_2.702273"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..775
                     /locus_tag="PKH07_17575"
     CDS             <1..775
                     /locus_tag="PKH07_17575"
                     /inference="COORDINATES: protein motif:HMM:NF021597.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2330 domain-containing protein"
                     /protein_id="HNT36803.1"
                     /translation="QQRAIIAWNGTEEILLLSTDQRATQRSAVLEVIPLPSRPSIKLG
                     SFATFEEAQKLVINKRMWVMAHGGKLAGNLQVPQQAARITFEKKMGAHDLTVAEVLEG
                     DAFVAFVQDYLKNKYGTQDAPIRPEFVKIIESYIEEGFKWFAFDVIVLGDKNQSREPI
                     EYRFASDRVFYPMRISTLERGETKVDLLVFTAQGANHFEGLPAKAIEREPQLSAVAED
                     FAGLEETWKKFFPAELPLKIDHWKVRGDIASFDKDILVR"
     gene            772..>1375
                     /locus_tag="PKH07_17580"
     CDS             772..>1375
                     /locus_tag="PKH07_17580"
                     /inference="COORDINATES: protein motif:HMM:NF024790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HNT36804.1"
                     /translation="MTRDPFYFSDDKRFTAEELAAWQDKEQCRERLARNRLKWYSLAL
                     QVGMFVLILLLWRLPFMMPIRLVVVLFHEMSHVIGAYLTGGVAFGIAIDPGGAGATLG
                     IGGNDVLVASAGYLGSLLIGVVLYCLSAAWEPKEAWAMLMALFCIALLFGWLNGFTAF
                     FGYLCIILMTMGFVLFRDGMKVFFIRVVSTTCCLFPIIDVM"
BASE COUNT          299 a          336 c          380 g          360 t
ORIGIN      
        1 gcaacagcgt gccatcatcg catggaacgg tactgaggaa atcctgctgt tgtccacgga
       61 tcaacgtgca acacagcgtt ccgccgtact ggaagtgatt cccctgcctt ccaggccgtc
      121 catcaaactg ggttccttcg cgacttttga ggaagcccag aaactcgtta tcaacaaacg
      181 aatgtgggtg atggcacatg gaggcaaact ggcgggcaat ctacaagtgc cacagcaggc
      241 ggcgcgaatc acttttgaga agaagatggg cgcccatgat ctgactgttg cagaggttct
      301 cgaaggggac gcctttgtcg ccttcgtgca ggactatctc aagaacaagt acggcaccca
      361 agatgctcca atccgtcctg agtttgtcaa aatcatcgaa agttacattg aagaaggttt
      421 caagtggttt gctttcgacg tcatcgtcct gggcgacaag aatcagagcc gagagccaat
      481 tgaataccgc tttgcctcag atcgagtgtt ctatccgatg cggatttcca cgttggaacg
      541 cggtgagacg aaggtggatc tccttgtgtt cacagcacag ggtgcgaacc actttgaagg
      601 acttccggca aaggccattg agcgtgaacc ccaactgagt gctgtggctg aggattttgc
      661 aggacttgaa gagacgtgga agaagttttt ccctgcggaa ttgcctctca agatagacca
      721 ttggaaagtc cgaggcgaca ttgcttcgtt tgacaaggac atcttggttc gatgacgaga
      781 gatcccttct atttcagcga cgacaagcgc ttcaccgctg aagaacttgc cgcttggcaa
      841 gacaaggaac aatgtcgcga acgactcgcg cgtaatcggc tgaagtggta ttctctcgca
      901 cttcaggtgg ggatgttcgt tttgatcctt ctcctatggc ggctcccctt catgatgccc
      961 atccggcttg tcgttgtcct ttttcacgag atgtcacatg tgatcggcgc gtatctgacc
     1021 ggtggtgttg cttttggcat cgccattgat ccgggtggag ctggagcaac actgggcatc
     1081 ggtggcaatg acgttctcgt cgcttcagcc gggtatttgg gttccctgct gattggagtt
     1141 gtgctctatt gtctgagcgc ggcctgggag ccaaaggaag catgggcaat gcttatggcg
     1201 cttttctgta ttgccttgtt attcggttgg ctcaacgggt tcactgcgtt cttcggctat
     1261 ctctgtatca tcctgatgac aatgggattt gtgctcttca gagacggaat gaaggtcttt
     1321 ttcatccgcg tggtctcaac aacctgttgt ctgtttccca tcatagacgt gatgg
//
LOCUS       DAOQUC010001282         1374 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_134878_length_1374_cov_2.705838, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001282 DAOQUC010000000
VERSION     DAOQUC010001282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1374
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_134878_length_1374_cov_2.705838"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..754)
                     /locus_tag="PKH07_17585"
     CDS             complement(<1..754)
                     /locus_tag="PKH07_17585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36805.1"
                     /translation="MHWSGWSVVMVALLFVVGEASAKQATREKYTHHGFWYGRVEMKT
                     VDAEDFLIMPWGWTAGDREVLEDIKDCGFNMAGFLAPEHVKLAVRVGLKCIVDDPNVS
                     RVVTDGQMSDEEIAKRVEKATARFKNNPDVYGYYVMDEPTAPLFPNLARWAAAITKAH
                     PGSTPYINLLPIGAQGPGSKDYEDYIEQFVSVIKPTYISYDHYTLMDDGSVRPSLYMN
                     LEVMRKASLNHGIPFWNIVLGNSHFHYAEVTQG"
     gene            complement(783..>1374)
                     /locus_tag="PKH07_17590"
     CDS             complement(783..>1374)
                     /locus_tag="PKH07_17590"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNT36806.1"
                     /translation="ENNTIVIFTSDHGEMLGAQGRRAKNIFYEEAVRVPFLMRWPERI
                     QSGRMSDVCLNTPDIMPTLLGLVDVPIPEKVEGMNLSHCALGQPGTEPEAALMQNTGA
                     CAVWEDGFEWRALRDKRYTYAVYRRDGKELLFDNQNDPYQMTDLAESPEHTTVMEHFR
                     RMLKRRMEELDDNFEASSWYRDHWTKDRIILIPRMR"
BASE COUNT          309 a          414 c          319 g          332 t
ORIGIN      
        1 cgccctgggt gacttccgca tagtggaaat ggctgtttcc caaaacgatg ttccagaaag
       61 ggattccatg gttcaaagaa gcctttcgca tgacttccag gttcatatac aacgacggac
      121 gtacggaacc atcatccatc aaggtgtagt gatcgtaact gatgtacgtc ggtttgatca
      181 cagacacaaa ctgctcgatg tagtcttcgt agtccttcga tccgggtccc tgagcgccaa
      241 tgggcaggag attgatgtac ggagtcgagc cgggatgcgc tttggtgatg gctgctgccc
      301 agcgcgcaag attcgggaaa agcggcgcgg ttggttcatc catcacgtag tagccataga
      361 catctggatt gttcttgaaa cgcgccgtgg ccttctcaac gcgtttggca atctcttcat
      421 cactcatttg gccatcggtg acgacacgcg agacgttcgg atcgtccacg atgcatttca
      481 gccccactcg taccgccaac ttcacatgct caggagctag aaatcccgcc atgttgaagc
      541 cacaatcctt gatgtcttcc aacacttcgc gatcaccagc agtccatccc caaggcatta
      601 tcaggaagtc ttccgcatca actgtcttca tttcaaccct cccataccag aacccgtgat
      661 gagtgtactt ctcccgcgtc gcctgctttg cggaagcctc tcccacaaca aacaacagag
      721 caaccatcac gacagaccaa ccactccagt gcatttcttg ctcctccttc gtgccacgtc
      781 tttcagcgca tccttgggat caaaatgatc ctgtctttcg tccaatgatc acgataccaa
      841 ctacttgctt cgaaattgtc gtctaactcc tccattcgcc tctttagcat cctgcgaaag
      901 tgctccatga ctgtagtgtg ttccggactc tctgccaagt cagtcatttg atacggatca
      961 ttctggttgt cgaaaagcag ttccttgcca tctcgacggt agacggcata cgtgtagcgc
     1021 ttgtctcgaa gcgcccgcca ttcgaagcca tcttcccaga ccgcgcaggc accagtgttc
     1081 tgcatcaacg ccgcttctgg ctcggtgccg ggctgaccca gagcacaatg gctcaggttc
     1141 attccctcaa ccttctcagg aatgggcaca tccaccagac cgaggagcgt cggcataatg
     1201 tccggagtgt tcaagcagac atcactcata cgccccgact ggattcgttc aggccaacgc
     1261 atcaggaacg gcacgcgaac cgcctcctcg tagaagatat tcttcgcgcg acgcccttgc
     1321 gcaccgagca tctctccgtg gtcagatgtg aagatcacga tggtgttgtt ctca
//
LOCUS       DAOQUC010001283         1373 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_135007_length_1373_cov_3.362671, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001283 DAOQUC010000000
VERSION     DAOQUC010001283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1373)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1373)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1373
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_135007_length_1373_cov_3.362671"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1282)
                     /locus_tag="PKH07_17595"
     CDS             complement(<1..1282)
                     /locus_tag="PKH07_17595"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNT36807.1"
                     /translation="MLTKTLLDDAIPHARYDLLFYIFIALVALHLGSTGLGIIKTRMA
                     TRVSCRVTYDLREQMFRQLQAMSVSYYDKNQVGSLMARITRDTEELQSFITQITTGIF
                     FNILTIIGVGCMLLYLDWRLCLYVVLPWPFVVFGTLRFWKNMVPRIKRLIYARWRISA
                     GLNSTLSGIRVVKAFAQEEHEEERFRNRNAMVFESNVDVQNYWNTYYPLITFFFPVGG
                     FLVWYLGGREAANNVITIGSLVAFISLLGRFQAPLMALTQTSNQMTRFLTAAQRIFEI
                     LDQTPDISDDPEAVELDAVQGEVEFKNVSFGYLPNQLILQDVSFRLRPGEMLGIIGPS
                     GAGKTTLINLICRFYDTDEGHVLVDGVDVRKITKDSLRKHIGLVLQEPFLFRGTIGEN
                     IAYGKPDATREEIIRAAKAANAHEFIVKLPEGYDT"
BASE COUNT          353 a          353 c          368 g          299 t
ORIGIN      
        1 gagtatcata tccttcagga agcttgacga tgaattcatg tgcattggcc gctttggctg
       61 cacggatgat ctcctctcgg gtcgcatccg gtttgccata ggcgatattc tctccgatgg
      121 tgccccgaaa cagaaaaggc tcctgcagaa ccagaccaat gtgtttgcga agtgaatctt
      181 ttgtaatctt tcggacatcc acaccatcta caagcacatg cccttcgtct gtatcataga
      241 accggcagat caggttgatc agcgtggtct ttccagcacc gctgggccca atgattccca
      301 gcatctctcc aggacgaagt cgaaaggaca catcctggag aatcagctgg ttgggaagat
      361 agccaaagga tacattcttg aactcgactt caccttgaac cgcgtccagt tccacggctt
      421 ccgggtcgtc actgatatca ggagtctgat ccaggatttc gaagatgcgt tgggctgcgg
      481 tcagaaatcg tgtcatctgg ttgctggttt gagtcaatgc catcagggga gcctggaacc
      541 gtcccagcag agagatgaag gcaaccaaag aaccaatcgt gatgacgttg ttggcggcct
      601 cgcgtcctcc gagataccag accaggaatc cgccgacagg gaaaaagaat gtgatcaggg
      661 gatagtaagt gttccagtag ttctgaacat ccacgttgga ctcaaagacc attgcatttc
      721 gattgcggaa tcgctcctcc tcgtgttctt cctgtgcaaa agctttcacg acccgaatcc
      781 cagacagggt ggagttcagg ccggcactga tacgccaacg cgcgtagatc agccgtttga
      841 tgcggggaac catattcttc cagaatcgaa gcgtgccaaa gaccacaaac ggccacggca
      901 gcactacata aagacacaga cgccaatcca gatacaacaa catgcagccg acgccgataa
      961 tcgtcagaat gttgaagaag atgcctgtcg tgatctgagt gatgaaactc tggagctctt
     1021 cggtgtcacg agtgatacgc gccataaggg aaccgacctg gttcttgtcg taatagctga
     1081 cagacatggc ctgcaactgg cggaacatct gttcccgcaa gtcgtacgtc acccgacagc
     1141 tgactcgcgt ggccattcgc gttttgatta tgccgagtcc ggtgcttccc aggtggagcg
     1201 cgacaagcgc aatgaagatg tagaaaagca gatcatatcg cgcatgggga atggcatcgt
     1261 caagaagagt cttggtcagc atgggaggaa ccagattcat ggcggtgctg gccaggagca
     1321 tgatcagaac cagcagcatc tgtggccaat acggtcggga gaccataaac agg
//
LOCUS       DAOQUC010001284         1372 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_135164_length_1372_cov_3.335611, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001284 DAOQUC010000000
VERSION     DAOQUC010001284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1372
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_135164_length_1372_cov_3.335611"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..512
                     /locus_tag="PKH07_17600"
     CDS             <1..512
                     /locus_tag="PKH07_17600"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HNT36808.1"
                     /translation="TFHVRGSGSAREWNDENHKAFLRSFARLADKGRDIGIPIMLENM
                     LPGRFTAREEELGALVEEFSSNDVGVCIDTGHAQLNGNLAGFVEQFASRAGVLHLQDN
                     SGGNGDEHLIPGKGSILWSDFVTGIKAGGFRGEKVVEVRSIGTLSETLAAIQQSIVET
                     DLHRMNNGL"
     gene            596..943
                     /locus_tag="PKH07_17605"
     CDS             596..943
                     /locus_tag="PKH07_17605"
                     /inference="COORDINATES: protein motif:HMM:NF025442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4258 domain-containing protein"
                     /protein_id="HNT36809.1"
                     /translation="MTNARKRESAEVTVRLQQQAFTDSIRVTVHGHQEMVAEEIAYQD
                     VREALLTCEVIEDYPDHQRGPCCLVCGRTSSGRFLHVVCTTTLKVIVIITVYEPKLPK
                     WPTPFERGGRHEM"
     gene            933..1157
                     /locus_tag="PKH07_17610"
     CDS             933..1157
                     /locus_tag="PKH07_17610"
                     /inference="COORDINATES: protein motif:HMM:TIGR03831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiT-type zinc finger protein"
                     /protein_id="HNT36810.1"
                     /translation="MKCSIQGCVGQYEPRQIVHTLQRGAEIFVFEHVPAEVCSVCGDT
                     LLSPQTINHLEDLLRTKSKPCRTAAVYEYS"
BASE COUNT          314 a          345 c          387 g          326 t
ORIGIN      
        1 tgacctttca tgtgagaggc tcgggttccg cccgcgaatg gaacgatgag aaccacaagg
       61 cttttctgag atcatttgcg cgacttgctg acaaaggcag agatattggc atccccatca
      121 tgcttgagaa catgcttccc ggtcggttca cggcgcggga agaagaactg ggcgcgctgg
      181 tggaagagtt ttcttccaat gatgtcggcg tttgtattga tacgggacat gcacagttga
      241 atggcaatct ggcaggcttt gttgagcagt tcgcttctcg ggcaggcgtg cttcatctgc
      301 aggacaacag cggtggcaac ggagacgaac atctcatccc tggcaaaggc tccatactct
      361 ggagtgattt cgtaacgggg atcaaggctg gcgggtttcg tggtgagaag gtggtggaag
      421 tccgatccat cggtacgctg agcgaaacgc tcgctgcaat ccaacagtcc atcgttgaaa
      481 cagatctgca tcgaatgaac aacgggttat gattctctgg cctggattgc cctcaaccca
      541 agctactgac ttgagattga ttgaccatac catgccaaag cggtaagatt cctgcatgac
      601 gaacgcacgg aagcgagagt cggctgaggt gaccgttcga cttcagcagc aggcattcac
      661 ggatagcatc cgcgtgacgg ttcacggtca tcaggaaatg gttgcggagg agatagccta
      721 tcaagacgtc cgcgaggccc ttttgacatg cgaggtcatc gaggattacc cagatcacca
      781 gcgcggtcca tgttgtcttg tctgtggtcg cacttcatcg ggacgttttc ttcacgtcgt
      841 gtgtacgact acgttgaaag tcattgtcat catcacggtg tatgaaccca aactacctaa
      901 gtggcccaca ccgtttgaga gaggaggccg ccatgaaatg tagtattcaa ggctgtgtgg
      961 ggcaatacga gcccaggcaa atcgtacata ccttgcagcg aggtgctgag atcttcgtct
     1021 tcgagcatgt tccagcggaa gtctgctccg tctgcggtga tacactcctt tctccacaga
     1081 cgatcaacca tctggaggac ttgctccgca cgaagtcgaa accgtgcaga acagccgcgg
     1141 tgtatgagta ctcgtagggt gtggccctga ttcgccggat gcacgagacg gaaaggttca
     1201 tagtgtgcgt tcctgagtcg tcgaaagacc gtagcgtctt tcttcggaat ggtgcgttcc
     1261 gacgtcttct ttgaatcacc aatggtagaa ctcgcagact tgttgctacc ccacccaagc
     1321 cggatgacac tttgcctgca ctggtcttcc ctttgcaaca gattgactga gc
//
LOCUS       DAOQUC010001285         1372 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_135207_length_1372_cov_2.545178, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001285 DAOQUC010000000
VERSION     DAOQUC010001285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1372
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_135207_length_1372_cov_2.545178"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            227..805
                     /locus_tag="PKH07_17615"
     CDS             227..805
                     /locus_tag="PKH07_17615"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNT36811.1"
                     /translation="MKKGFTLIELLIVVAIIGILAAIAIPNFLMAQIRAKVANTRAEQ
                     QTLGTALEAYRVDNSAYARDQPYWQWEHGVPATLISVKALSALTTPVSFIKSVPQNPF
                     GNKVDQENDLWAYFRYYSEYWKGFTSMTRPATPRIWSLTSCGPNAVTNFGEYLIYGEA
                     VVNSINIWGFRGCIYDPTNGVVSDGDIVRIGP"
     gene            1052..>1372
                     /locus_tag="PKH07_17620"
     CDS             1052..>1372
                     /locus_tag="PKH07_17620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNT36812.1"
                     /translation="MKRSHFQERGFTLIELLIVVAIIGILAAIAIPNFLQAQTRSKVA
                     RVKSDMTTIVTCMETYRVDNNEYPATQFSQESRYAFLTSPISYMRSIPADPFGSGVPG
                     TWVRD"
BASE COUNT          317 a          300 c          383 g          372 t
ORIGIN      
        1 aattatcttg ggcagtccgg tattcattgg gaaggcaagt ccaggcgggt tcctgttggt
       61 tggacaagaa gaattgcgtt catgcagtta ggagcaggct agaatctttg agtgtgcggg
      121 ctgtacccac tccatcggag gcctgctcgg tgaatgagga ttcttctttg actgagtgca
      181 aactggagag tgtttcagag agatcgtaag gaattggagg atgttcatga aaaagggttt
      241 tacgttgatt gaactgctca ttgtcgttgc catcatcgga atactggctg caatagcaat
      301 tccgaacttc ctgatggcgc agatccgtgc caaggttgcc aatacaaggg cggagcagca
      361 gaccttggga acggcgcttg aggcgtatcg agtggacaac tcggcatatg cgagagacca
      421 gccttactgg cagtgggagc atggagttcc ggcgactctg atcagtgtga aagctctttc
      481 tgcgctgacg acaccagttt cgttcatcaa gagcgttccg cagaatcctt ttggcaacaa
      541 agtggatcag gaaaatgacc tctgggctta ttttcgatac tactccgagt actggaaagg
      601 tttcacctcg atgacacgtc cggcaactcc gcggatctgg tctctgacca gctgcggtcc
      661 gaatgctgtc accaacttcg gagagtacct catctacggc gaagcagtcg tgaactccat
      721 caacatctgg ggtttccgag gatgcatcta cgatccgaca aatggtgttg tcagcgatgg
      781 cgatattgtg aggattggcc cataggacgg tctgctccat ttctcggtgc agtcaagagt
      841 cattgttttt acggttcatc ttggtgcccg tttttttgtc tgttcttctt tttctcttga
      901 cagtaaacgc tttcgttgta cattcactac aggcagattg gagtttgttg ggaaggctta
      961 gttatacggg ttccaagcat attgacgctt ggaaagatgt cgaggagaga actgctctca
     1021 ggatgatgag caggctgaat ggaggtaaga aatgaaacgt tcgcattttc aggagagagg
     1081 attcactctg attgaactac tgatcgttgt tgccatcatt gggattcttg cggcaattgc
     1141 tatcccgaac tttctgcagg ctcagacccg ttcaaaagtt gcgcgggtca agtcggatat
     1201 gacgacgatt gtcacgtgca tggagaccta tcgcgtggat aacaacgaat atcctgcaac
     1261 tcagttttcg caggagagtc gttacgcctt cttgacttcg ccgatttctt acatgaggag
     1321 tattccggcc gatccgtttg gcagtggggt tcccggaaca tgggtcagag ac
//
LOCUS       DAOQUC010001286         1369 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_135649_length_1369_cov_3.777778, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001286 DAOQUC010000000
VERSION     DAOQUC010001286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1369
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_135649_length_1369_cov_3.777778"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..904
                     /gene="dxr"
                     /locus_tag="PKH07_17625"
     CDS             <1..904
                     /gene="dxr"
                     /locus_tag="PKH07_17625"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006718253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HNT36813.1"
                     /translation="LAQLSEADLVLVATVGAIGMVPTIAALKAGKSVALANKEALVMG
                     GDLVMKEAGSVGPRLIPVDSEHSAIFQCIGQHPTHEVSRILLTGSGGPFRKWSDQDLQ
                     TVTVAQALNHPNWSMGNKITIDSATLMNKGLELIEAMHLFGLPADRIRVVVHPQSIVH
                     SLVEFQDRSVLAQMGLPDMRLPIQYSLSYPERWGAEYPCLDLFAAASLTFEEPDLERF
                     PCLRLAYEAARTGGTMPAQLSVCNEEAVHAFLKERIGFCDIARIIETVLERLSVEESE
                     SVEQLLDLEQLVRAQAREEIERFS"
     gene            928..>1369
                     /locus_tag="PKH07_17630"
     CDS             928..>1369
                     /locus_tag="PKH07_17630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36814.1"
                     /translation="MIHLKRLISCRALLALCCVCLCASGCRSLPTRSKGRYWLSGMNI
                     VWFTTSANTSRLVNAEAIRESFALCAKSGLTAVVIEMETRGYLTYPSQLGPHFSQMPN
                     SHFAPDYDLIATATQQAHELGLKCFVSYFGTHTSPQNDSCRFVHF"
BASE COUNT          283 a          359 c          398 g          329 t
ORIGIN      
        1 gttggcgcag ctctccgaag cggatcttgt tctggttgcc acggttggtg ctattggcat
       61 ggttccgacc atagccgcct tgaaggctgg caaatccgtg gcactcgcca acaaggaagc
      121 gctggtcatg gggggcgacc tggtgatgaa ggaagcaggc agcgtcgggc cgcggctcat
      181 ccccgtggac agtgaacaca gcgccatctt ccagtgtatc ggtcagcatc caacgcatga
      241 ggtttccaga attctgctca ccggatcggg gggtcctttt cggaaatgga gtgaccagga
      301 tctgcagaca gtcaccgtcg cgcaggcgct caaccatccc aactggtcta tggggaacaa
      361 gatcaccata gactctgcaa cactgatgaa caaaggtttg gaactgattg aggcaatgca
      421 tttgtttgga ctgcctgccg atcggattcg cgtggtggta catccgcagt ccattgtgca
      481 ttcactggtc gagtttcagg atcggtcggt gctggcacag atgggtctac cggatatgcg
      541 tcttccgatt cagtattcgc tgtcgtatcc agagcgctgg ggagcggagt acccgtgtct
      601 ggatcttttt gcggcggcaa gcctcacctt tgaagagcct gacctggagc ggtttccgtg
      661 cctgcgtctg gcttatgagg cggctcggac tgggggcacg atgcccgcgc agttgtccgt
      721 ctgcaacgaa gaggcagtcc acgcgttctt gaaggaacga attggatttt gtgatattgc
      781 acggatcatc gaaacggttc tcgaacggct ttcggtcgaa gaatccgagt ccgtggagca
      841 actgttggat ctcgagcagc tcgttcgagc acaagcccga gaggaaatcg agcggttttc
      901 ctagtcgagc gttggaagga tgtttgaatg attcatctca agagactcat ctcgtgccga
      961 gccttgttgg cgctctgttg cgtttgtctt tgtgcctcag gatgtcggag tctgcccaca
     1021 cgttcaaagg gacgatactg gctgtcggga atgaacattg tctggttcac cacgtcggcc
     1081 aacacgtccc gacttgtgaa cgctgaagcc attcgggaaa gttttgcgtt gtgcgcaaag
     1141 tccggtctga ccgcggttgt catcgagatg gaaacacgcg ggtatctgac ctaccccagc
     1201 cagttaggcc ctcatttcag ccagatgccc aattcccatt ttgcacccga ctatgacctg
     1261 atcgccaccg ccacacaaca agcccatgaa cttggtctga agtgcttcgt aagctatttc
     1321 ggaacgcata ccagcccaca gaacgacagt tgtcgttttg tgcattttg
//
LOCUS       DAOQUC010001287         1369 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_135659_length_1369_cov_3.420091, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001287 DAOQUC010000000
VERSION     DAOQUC010001287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1369
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_135659_length_1369_cov_3.420091"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1369
                     /locus_tag="PKH07_17635"
     CDS             <1..>1369
                     /locus_tag="PKH07_17635"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNT36815.1"
                     /translation="ELMATLKPFGCNLVKYLGDQGFVISFEQGADINRVKAQTGIQWI
                     VPLHSRIKMDSNLAAEGIIEDVPILVHLFPDASSGNVCKSIERLLPDQPVQVGRSRIG
                     FRCPRGKLLGVVSQIADLEDVFFVERDRGVVLLNRDAIRILQSGVYTGAAALFEKGLF
                     GQGQIAAVCDTGIDADSCFFRDPSGTLPPTNLAFGTVVEATRRKVIAVNFLYSADQPD
                     NSTHWDNQGHGTSVAGCIGGSHISDPTSSTNYNGMAPMAQFVIQDAGFQYDDCADLPG
                     LGCPVIDLAPVFEQTYVQGARLHNNSWGDRENDLPHNTYTARCQDVDLFTWNHKDFLV
                     VCAAGNEGTAFGAVTSPSVAKNALSVGGTNNGAQADSIIYFSSRGPASDGRIKPDLVA
                     PASVLTARSDRNVLTGNCDLNSTQGTSFASPLTVGAACLVREYLQKGYAPTGVPRQEN
                     RMDSAS"
BASE COUNT          300 a          376 c          379 g          314 t
ORIGIN      
        1 aggaactgat ggcgacactg aagccgtttg ggtgcaacct ggtgaaatac ctcggcgatc
       61 aagggtttgt gatctcattc gaacagggag cagacattaa ccgagtcaaa gcccagacag
      121 ggattcagtg gattgtgcct ctgcattctc gcatcaagat ggactcaaat ctggccgccg
      181 agggaatcat cgaggacgtg cccattctcg tacacctctt tcctgacgcc tcttcaggca
      241 atgtctgcaa gtctatcgag cggctgcttc cggatcaacc tgtccaagtt ggcagaagtc
      301 gcatcgggtt tcgttgtcct cgtggaaagt tgctcggagt agtctcccaa attgccgact
      361 tggaagatgt cttctttgtg gaaagggaca ggggtgttgt cctgctgaat cgggatgcca
      421 tccggattct ccaatcggga gtgtataccg gcgcggctgc tttgtttgag aagggactct
      481 tcggacaagg ccagattgcc gcggtgtgtg acacagggat agatgcggac tcctgttttt
      541 ttcgggatcc gtccggcact ctgcccccca caaatctggc ctttggaacc gttgttgagg
      601 caactcgaag aaaagtcatc gccgtgaatt tcctctactc ggcagatcag cccgacaatt
      661 cgacccactg ggacaatcaa ggtcacggga cttccgtcgc cggctgcatt ggtggaagtc
      721 acatttccga tccgaccagc agcaccaact acaatggaat ggcaccaatg gctcaattcg
      781 tgatccagga tgcgggtttt cagtatgacg actgcgctga tctgccgggt cttggatgtc
      841 ccgtcattga tcttgctccc gttttcgagc agacctatgt tcaaggagca cgactgcata
      901 acaacagctg gggcgacaga gagaacgatc tccctcacaa cacctacacc gcacgctgtc
      961 aggatgttga tctgttcacc tggaaccaca aggactttct tgtcgtctgc gccgcgggca
     1021 atgagggaac tgccttcggt gcagtgacca gcccgagtgt tgccaagaat gcgctcagcg
     1081 tgggtggcac gaacaacggc gctcaggccg attcgattat ctatttctcc agccgtgggc
     1141 cagccagcga cggcagaata aagcccgatc tggtcgctcc ggcttcagta ctcacagccc
     1201 gctccgatcg caatgtgctg acgggcaact gtgatctgaa ctcaacgcaa ggaaccagtt
     1261 ttgcatcacc gctcacggtg ggagccgcct gtcttgtgcg tgaatacctt cagaagggct
     1321 atgctccaac gggtgttccc aggcaggaga accgcatgga ttcagcctc
//
LOCUS       DAOQUC010001288         1367 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_135991_length_1367_cov_3.855183, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001288 DAOQUC010000000
VERSION     DAOQUC010001288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1367
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_135991_length_1367_cov_3.855183"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..688
                     /locus_tag="PKH07_17640"
     CDS             <1..688
                     /locus_tag="PKH07_17640"
                     /inference="COORDINATES: protein motif:HMM:NF014554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate--acyl-ACP acyltransferase"
                     /protein_id="HNT36816.1"
                     /translation="SLFELGRLEGVSRPALATIVASRKQPTVLVDVGANVDCKPQWLA
                     QFAIMARAYVQSVFQIENPTVGLLSIGEEAEKGNEQTLATYKILQDADISFIGNVEGH
                     DITLGKANVIVADGFVGNVILKLAEGLSEMILHTLKSELAELRQRVEFSPDDTLAHFL
                     KQLDYAEYGAALLLGVNGNSLIGHGKSSPKAVANALRQAYRSVHYDMNDQIVAEIKRC
                     NARMPVAGQA"
     gene            837..>1367
                     /locus_tag="PKH07_17645"
     CDS             837..>1367
                     /locus_tag="PKH07_17645"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase 3"
                     /protein_id="HNT36817.1"
                     /translation="MDNRVYTRIVGTGSCVPPKVLTNHDLEKIVDTSDQWIVERTGIS
                     ERRVTDESTAASDLAIEASKKALEMAGIAAEEVDLILVATVTPDMFFPSTGCIVHKAL
                     TSKPIPAFDLSAACSGYIYGLSVADAYIRSGMAKTILLIGADTLCKMLDWTDRNSCVL
                     FGDGAGAVVLEASEKPG"
BASE COUNT          313 a          358 c          383 g          313 t
ORIGIN      
        1 ttccctcttt gaactgggtc gtcttgaagg ggtttccaga ccggcactgg caacaattgt
       61 tgcatccagg aagcaaccaa ccgttctcgt ggatgtgggc gccaatgtgg actgtaagcc
      121 tcagtggctc gcccagtttg ccatcatggc gcgtgcctat gtgcagagtg tttttcagat
      181 tgagaatccg acggtcgggc tgctcagcat cggtgaggaa gcggaaaagg gcaatgaaca
      241 gaccttagcc acctacaaga ttctccagga tgccgacatt tctttcatcg ggaatgttga
      301 aggacatgac atcactttgg gcaaggcaaa cgtcatcgtg gcggatggct ttgtcggcaa
      361 tgtcattctg aaacttgcgg aaggcctttc cgagatgatt ctgcacacgc tcaagagtga
      421 actggccgaa ctgagacagc gggttgagtt ctcgccggac gacactctgg cgcattttct
      481 caaacaactc gactatgccg agtacggcgc tgctctgctt ctgggtgtca atggcaacag
      541 tctcatcgga catggcaagt cgtctcccaa ggccgttgcc aatgcgctcc gtcaggcata
      601 ccgatctgtt cactatgaca tgaacgatca gatcgttgca gagatcaaga ggtgcaatgc
      661 tcggatgccc gtggcgggac aagcctgatg cgaggggaca ttccatcagg cctcactcca
      721 aggcagtcgg cgggtacgtt gcagtcatcc aggccggact gtcggaaaac agagcagatc
      781 acagcacggg tgtagtagaa aacccaggct gtgttttttt caacgaggag aggattttgg
      841 acaacagagt ttatacacgg attgtcggca caggatcatg tgtgccccca aaggttctca
      901 caaaccatga tctggagaag atcgtggaca cttcagatca gtggatcgtg gaacgaacgg
      961 gcatctccga aagacgggtc accgatgaat ccacggccgc ttcggatctc gccatcgagg
     1021 cttccaagaa agccctcgag atggcgggca ttgcggcaga agaagtggac ttgattctgg
     1081 tcgcgactgt cactccggac atgttcttcc cttcaacagg ttgtattgtt cacaaggcct
     1141 tgacttccaa gcccattccc gcttttgacc tgtccgcagc atgttccggc tacatctatg
     1201 gcctctccgt tgccgatgcc tacattcgca gcggcatggc gaaaacgatt ctgctcattg
     1261 gagccgacac gctctgcaaa atgctcgact ggacagatcg aaactcctgc gtcttgttcg
     1321 gcgacggtgc tggtgctgta gtgctggagg cttcggaaaa acccggc
//
LOCUS       DAOQUC010001289         1367 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_136103_length_1367_cov_2.059451, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001289 DAOQUC010000000
VERSION     DAOQUC010001289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1367
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_136103_length_1367_cov_2.059451"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..753
                     /locus_tag="PKH07_17650"
     CDS             <1..753
                     /locus_tag="PKH07_17650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36818.1"
                     /translation="AVGSNGSVAHSADINTWTTLGSAATGTTETLNAIASSGGTLVAV
                     GNNGTLRYSTNGTTWTTPASISEERMGRPTPTVPPQIAQRREVRMPQQVAQPIVPEAP
                     RPTIRPGKVIRSNPSPIEADLKPVALASLIRSGSVTVQGWNADYFRTVDGDTTSLYRT
                     DQVNPLVITFAFEEAINLKTVRIFPSYSSYDWAVYTDASEQGLIIRGVPEEQWSRMDF
                     EQPLQTKSIRIEVLRVERDNYVHLNEIEIYVQ"
     gene            939..>1367
                     /locus_tag="PKH07_17655"
     CDS             939..>1367
                     /locus_tag="PKH07_17655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36819.1"
                     /translation="MRILIGVDDTDNETSIGTGRFARDLSEALVKAGLGTAHGVTRHQ
                     LLVDSRIPYTSHNSAAAILFETSDVDAAARCCRGFYADNFHSGSDPGLCIAEDACSCL
                     TEFGRAAQREILTKSQAFDLAYASGCILEEHGGTGDGIIGA"
BASE COUNT          311 a          379 c          390 g          287 t
ORIGIN      
        1 gcggtgggca gcaatggcag cgtggcgcat agcgccgaca tcaacacctg gaccaccctc
       61 ggcagcgccg ccaccggcac caccgaaacc ctcaacgcca tcgccagcag cggcggcacc
      121 ctggtggcgg tgggcaacaa cggcaccctg cgctactcca ccaacggcac cacctggacc
      181 accccggcct cgatcagcga agagcgcatg ggacgtccaa cgcccactgt cccaccgcag
      241 atcgcgcaga ggcgggaagt ccgaatgcca cagcaagttg cacaaccgat cgtccctgag
      301 gcaccgcgtc cgaccatccg gccgggaaaa gtgatccgaa gcaatcccag cccgatcgag
      361 gctgacttga agccggttgc cctggcaagc cttatccgct cgggatccgt gacggtgcaa
      421 ggctggaatg cggattactt caggacagtg gatggggaca caaccagtct ctatcgaacc
      481 gatcaggtga atcctctcgt gatcaccttt gcctttgagg aggcgatcaa cctgaagacg
      541 gtgcggatat ttccttccta ttccagttac gattgggctg tctacacaga tgcttcggag
      601 cagggcttga tcatcagggg tgttcccgag gagcaatggt cgcgcatgga cttcgaacag
      661 cccttgcaga ctaagagcat tcggatcgag gttcttcggg ttgaacgaga caactatgtt
      721 catctgaacg agattgaaat ctacgttcag tgaatcaagg ccggtatgcc tgtgctatcg
      781 cattgtcgca ctttgggcac cttgagatct gagatttcaa ccccttgaca aggggagaag
      841 ccacaggcat gatagaagaa tgagagtgtt tttttttcta atccgttatg caccttgacg
      901 cataacgaac gcatagagga ttctgtaggc gctttccgat gagaattctg atcggtgttg
      961 acgacacgga caacgagaca agtattggaa cgggtcgctt tgcgcgagac ctttccgagg
     1021 ctcttgtcaa agcaggactg ggaaccgcac atggggtgac ccggcaccag ttgcttgtag
     1081 attctcgcat tccatataca tctcacaaca gtgctgcggc catccttttt gaaacttctg
     1141 acgtggacgc agcagcgcgg tgctgccgcg gattttatgc cgacaatttc cattccggat
     1201 cggatcccgg gttgtgcatc gcagaagacg cttgttcttg tttgacggag ttcgggcggg
     1261 ctgctcagcg tgagattctg acaaaatccc aagccttcga cttggcgtat gcatcgggat
     1321 gcatcttgga agagcatgga ggaacgggag atggtatcat cggcgcg
//
LOCUS       DAOQUC010001290         1366 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_136208_length_1366_cov_2.790236, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001290 DAOQUC010000000
VERSION     DAOQUC010001290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1366
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_136208_length_1366_cov_2.790236"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1366
                     /locus_tag="PKH07_17660"
     CDS             <1..>1366
                     /locus_tag="PKH07_17660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HNT36820.1"
                     /translation="RLATGLTFLLLAAVVAHAGPLEFVRERVVANGSPAMASIGTGIP
                     SKGETVDLALTLANTGSAAARTVTARLTSVTPEFAQILTSTATFGLINAGAEKESLSN
                     VRILIDPLASDFSELTFSMVISESGGAQWPGQFSLSVLPSISVCPAVGNNQNPSYLAY
                     DRVANRLYCTNTQTGSVAVIDPQVNSAVAAFRLGTSPDIAAVDSLGKRLFVLDKNAAA
                     PAVHVVSTEDYSLITKINLPSLPSWITFNPDANKCYVSFDGTGTVRLLECGGYSLQDS
                     VAVGGKPAGSFVDSVSQTLQVVNYGQLAVHVIDLATRQILATLDLKQNIAGLWSGRNG
                     VYDADNRTLYLGASLVGSGLCIVRIRDGSSVDYLPFDGGGLTSIEGLAWDPSRSGLFV
                     VGAAETAFSRVVALAVEQGYVTPELNLQIEGLHSCAVHVPISRRVYIGNARKYRLDSL
                     DALNL"
BASE COUNT          280 a          355 c          372 g          359 t
ORIGIN      
        1 tagactcgcc acaggtctta cgttccttct attggctgcc gttgtagccc atgcggggcc
       61 gttggaattt gttcgagaac gcgttgtggc aaacggaagt cctgcgatgg ccagtattgg
      121 aacaggcatt ccaagcaagg gtgagacggt agatctggct ttgacccttg ccaataccgg
      181 ttccgctgct gctcgaacgg tgactgcacg tctaacttcc gtcactcccg aattcgcgca
      241 gattctgaca tcaacggcca cctttggact gatcaatgcc ggggctgaaa aggagtctct
      301 cagcaacgtt cggattctca ttgatccgtt ggcttcagac ttctccgaat tgactttcag
      361 catggttatc tcagagtcgg gtggtgcgca atggccgggg cagttctcac tctctgtcct
      421 gccctccatc agtgtgtgtc ctgcagtcgg caataaccag aacccgtcct acctggcata
      481 cgatcgagtc gccaacagac tctattgcac caacacgcag acgggaagtg ttgctgtcat
      541 tgatccccaa gtgaactctg cggtggctgc gttccgactt ggaacatcac cagacattgc
      601 agccgtggat agccttggaa agcgcctttt tgtgttggac aagaatgcgg ctgctccagc
      661 agttcatgtt gtcagcacgg aagattactc gctcatcacg aagatcaact taccaagtct
      721 gccttcatgg attacattca acccagacgc gaacaagtgt tatgtcagtt tcgatggaac
      781 aggcactgtg cgacttctcg agtgcggtgg ttactcattg caggactctg ttgcggtggg
      841 tgggaagcca gctggctcct ttgtggattc ggtctcgcag acacttcaag ttgtgaacta
      901 tgggcagtta gccgttcacg tcattgactt ggccactcga caaatcctcg caacactcga
      961 tttgaagcag aacatcgcag gcctttggag tggacgcaat ggagtctacg atgctgacaa
     1021 ccgtactctt tacttgggag cctctcttgt tgggtcgggt ctttgtattg tacgcatcag
     1081 ggatggttcc agcgtggact atctgccctt tgatggtggc ggattgacta gcatcgaagg
     1141 actggcctgg gatccaagcc gctccggtct ttttgttgtt ggcgctgctg agactgcatt
     1201 ttcgcgcgtc gtggcgctgg ctgttgaaca gggttatgtt acaccggaac tgaatcttca
     1261 gatcgagggt ctgcattcct gtgccgtcca tgtaccgatt tcgaggagag tctatattgg
     1321 caatgcacgc aagtatcgcc tggacagcct ggacgccctg aacctt
//
LOCUS       DAOQUC010001291         1365 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_136317_length_1365_cov_4.658015, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001291 DAOQUC010000000
VERSION     DAOQUC010001291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1365
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_136317_length_1365_cov_4.658015"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /locus_tag="PKH07_17665"
     CDS             <1..246
                     /locus_tag="PKH07_17665"
                     /inference="COORDINATES: protein motif:HMM:NF013286.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HNT36821.1"
                     /translation="KLTNLQKIALIQKVVQEEIRPVLAKDNGDLVLMDVVGNRVLVSL
                     RGSCAGCPVAPITLKSYVQEKLRELVSEDLVVEEVPS"
     gene            243..>1365
                     /gene="nifS"
                     /locus_tag="PKH07_17670"
     CDS             243..>1365
                     /gene="nifS"
                     /locus_tag="PKH07_17670"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011365756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HNT36822.1"
                     /translation="MNNIYVDNNATTQVAPEVFEEMKPYFCDLYGNPSSMHSFGGNVQ
                     RKVVEARERVADILGALPTEIVFTSCGTESDNAAIRSALECFPEKRHIVTTRVEHPAV
                     LNLCRHLEKHGYDVTYLSVDAEGRLNLDEVQDSLRSDTAIVSIMYANNETGVIFPIEE
                     IGLMVRQRGILFHTDAVQAVGKIPLNLRESTIEMLSLSGHKLHAPKGVGVLYVRRGTP
                     FRPFLIGGHQERGRRGGTENVASIIALGKACELAKDHLNEENTRVRALRDRLEKGLLE
                     NCPDARINGCTDQRLPNTLNISFEYVEGEAILLMINELGIAASSGSACTSGSLEPSHV
                     LRAMGIPFTCAHGSVRFSLSCYNTEQEIDYIVEKMPGIIR"
BASE COUNT          322 a          353 c          381 g          309 t
ORIGIN      
        1 aaactgacca accttcagaa aatcgccctc attcagaaag tcgtgcaaga ggagattcgt
       61 cctgtgttgg ccaaagacaa cggcgacctg gttttgatgg acgtggtcgg caaccgtgtg
      121 ctggtctccc tcaggggtag ttgtgcagga tgtcctgttg ctccgattac gctgaagtcc
      181 tatgttcagg agaagttgcg ggaactggtc tccgaagatc ttgttgtcga ggaggttccg
      241 tcgtgaacaa tatctacgtg gacaacaacg ctacgacgca agtggctccc gaagtcttcg
      301 aagagatgaa accgtatttc tgcgatctct acggaaatcc atccagcatg cacagttttg
      361 gcggaaacgt gcagcgcaaa gtggtggaag cccgagaacg ggtcgcagac attctgggcg
      421 cactgccgac agagatcgtt ttcaccagtt gcggaacgga aagcgacaac gccgccattc
      481 gtagcgccct ggagtgcttt cccgagaaga gacacattgt cacaacccgg gtcgagcatc
      541 ccgcagtgct gaatctgtgc aggcatcttg agaagcatgg ctacgatgtt acctatctct
      601 ctgttgatgc ggaaggtcgg ctgaacctcg atgaggtaca ggacagcctt cgcagtgata
      661 ccgccatcgt ttcaatcatg tatgcgaaca atgagactgg cgtcatcttt cccatcgagg
      721 aaatcggcct catggtgaga cagcgcggga ttctttttca caccgacgcc gtacaggccg
      781 ttggcaagat tcctttgaat ctgcgcgaga gcaccataga aatgctgtcg ctttcaggtc
      841 acaaactgca cgctcccaaa ggggtcggcg ttctgtacgt tcgcagggga acgccgtttc
      901 gaccttttct catcggaggg catcaggagc gtgggcgccg aggtggcacg gaaaatgtgg
      961 catccatcat cgctctgggc aaagcctgtg aactggccaa ggatcatctg aatgaagaaa
     1021 acactcgagt cagggcgttg cgcgaccgac tcgaaaaggg gcttctcgag aactgtccag
     1081 acgctcgcat caacggatgc accgatcaga gactccccaa cacgctcaat atctcttttg
     1141 aatacgtgga aggtgaggca atccttctca tgatcaacga gctgggtatt gccgcttctt
     1201 ccgggtcagc ctgcacttcg ggttctctgg agccgtcaca cgttcttcgg gcaatgggga
     1261 tacccttcac gtgcgcacat ggctctgttc gattcagtct gagctgctat aatacagagc
     1321 aggagataga ttatattgtc gagaagatgc ctgggatcat ccgac
//
LOCUS       DAOQUC010001292         1364 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_136495_length_1364_cov_3.592819, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001292 DAOQUC010000000
VERSION     DAOQUC010001292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_136495_length_1364_cov_3.592819"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..594
                     /locus_tag="PKH07_17675"
     CDS             <1..594
                     /locus_tag="PKH07_17675"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNT36823.1"
                     /translation="IKSLWRRFLEDEKTWHLFTLAPGGKRWHHAFLGGLLEHTCSVIK
                     LADFFARTYPAADRDLVLSGALFHDIGKVRELSYETSFDYTDEGRLLGHIVIGNEMVC
                     NYARSIEGFPEDKLLFLRHLILSHHDDLGGSPVVSMTKEAFLLHHADRLDSQFAAVCR
                     EIEEASAQPGGEWSGFINLLERQLYLGRSETSKDKER"
     gene            597..>1364
                     /locus_tag="PKH07_17680"
     CDS             597..>1364
                     /locus_tag="PKH07_17680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36824.1"
                     /translation="MHYRKLLRNTRHSIRRSFARQGLLALALTLLVLLGVLGALGLFD
                     PSREFVSVIFLLGLLLVVGLLIRFLVCPLLFPPSKRQLLLLLEESHPEFRGSLILADY
                     LETCPDEVEKLGYSTQLQKQILDFAHDLPEQLTKCVLPSRGSSSDTRRLFWIVLVADV
                     LLFSVRPGAFLLGWQRVHDVLRPSSVAITRILLDVEAPSRARRNGNVTIQARVAGSRS
                     VTPIVHFPSMDGQWNQVEMTSETPSRFSHEIARLVRST"
BASE COUNT          278 a          371 c          352 g          363 t
ORIGIN      
        1 atcaaatccc tctggcggcg attccttgaa gatgagaaaa cgtggcattt gttcactctg
       61 gctccgggtg gcaaacgctg gcatcacgcc ttcttgggcg gactcttgga gcacacatgt
      121 tccgtgatca aactggctga tttttttgca agaacctatc ccgccgcgga tcgcgatctt
      181 gttttgagcg gcgccctgtt tcacgacatc ggcaaggttc gtgagctcag ttatgagaca
      241 tcttttgact acaccgacga aggcaggctt ctgggacata ttgtcatcgg caatgagatg
      301 gtctgcaact atgctcgttc aattgaagga tttcctgagg acaaactcct cttcttgcgg
      361 catttgatct tgtcccatca cgatgacctc ggaggctccc ctgtcgtttc aatgacaaaa
      421 gaagcgtttc tgcttcatca cgcagacaga cttgattctc aattcgctgc ggtctgccgt
      481 gagattgaag aggcgagcgc acaacctggc ggagaatgga gcggcttcat caatctgctc
      541 gaacggcaat tgtatctggg tcgctctgaa acatccaaag ataaggaacg ctgacgttgc
      601 actacaggaa actgctccgc aacacccgac acagcatccg tcgctcgttc gcacgccaag
      661 gtcttttggc actggcgctg acgctgctgg ttctcttggg ggtgctcgga gcacttggcc
      721 tgttcgatcc gtccagagag tttgtctctg tgatctttct ccttggcctg ctgttggtcg
      781 taggattgtt gattcgattt cttgtctgtc ctcttctttt tccaccctcg aaaaggcaac
      841 tcctgctcct gttggaggaa tcacatcctg agtttcgtgg cagtctcatc ctggcggact
      901 atctggagac ttgtccggat gaagtggaga aactggggta ttcgacccaa ctccagaaac
      961 agattctcga ttttgcacac gaccttcccg aacaactgac gaagtgcgtc ttgccaagcc
     1021 gcggctcttc atccgatact cgacgactgt tctggatcgt acttgtcgcc gatgtcctgt
     1081 tgttcagcgt caggcctggg gcgttccttc ttggatggca acgcgtgcac gacgtcctgc
     1141 gtccctcaag tgtcgccata acccgcatct tgcttgacgt tgaggctccg agccgcgcgc
     1201 gaagaaacgg gaatgtgacc attcaggcgc gcgttgcagg aagcagatct gtcactccca
     1261 tcgttcattt tccttcgatg gatggacaat ggaatcaagt ggagatgact tccgagactc
     1321 ccagccgatt ttctcatgag atcgcgcgac ttgttcgttc caca
//
LOCUS       DAOQUC010001293         1364 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_136522_length_1364_cov_2.889228, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001293 DAOQUC010000000
VERSION     DAOQUC010001293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_136522_length_1364_cov_2.889228"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="PKH07_17685"
     CDS             complement(<1..329)
                     /locus_tag="PKH07_17685"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HNT36825.1"
                     /translation="MKIALISPYESTASVGVRTLSSVLRAAGFETKLFFIPLPFEVPY
                     DEVLMKGLSDLLNDCQVVGISLTSNYLDRAVQLTQWIRQTGSQRILWGGIHPTLEPQE
                     CLEHADFV"
     gene            complement(341..>1364)
                     /locus_tag="PKH07_17690"
     CDS             complement(341..>1364)
                     /locus_tag="PKH07_17690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36826.1"
                     /translation="LFEDLPENVWLTCLWGKSDSEIYVGLNNGQIRKYNGSAWSVEST
                     GTTSQIRAISGYGNYVYAVGDQSIAIYKSGNAWRSINGVENRSEYSLVAMTATADAAY
                     AVSTLYQGGSRIYKFLAGTATVELENLSDVFEDLCTTRSGIVYGMSSSGLVITTAPVP
                     ANRFTGVVNTDLTGWTNLGNSGVALRFTEHRGQQYAAAWRSASAPELFVTPGIADNVP
                     ASERWTVIQNMGYQNAHNLPPAQVQVKYSLDALPTGLSVEEAGLYRQDGNGWVGFPSR
                     SYPDQQLLQTETSSTLSVWTIARSAQFAQSLNPPRLINLIEDIARGEASYLDLFRRIP
                     TWMQSE"
BASE COUNT          310 a          365 c          366 g          323 t
ORIGIN      
        1 acaaaatcgg cgtgttcaag gcattcttgc ggttccagtg ttggatggat gccgccccac
       61 agaatccttt gcgatccggt ttggcgaatc cattgagtca gttgaacagc ccgatcgaga
      121 tagttcgatg tcaggctgat tccaacgacc tggcagtcgt tcaggaggtc ggagagcccc
      181 ttcatgagca cttcatcgta tgggacttca aaaggcaacg ggatgaagaa cagctttgtc
      241 tcaaaccctg ctgcacgcag cacggacgaa agggttcgaa ccccgactga ggcggtggac
      301 tcatacggag atatcaacgc tattttcatc ttgtcctatg tcactccgac tgcatccaag
      361 ttggaattct gcgaaacaga tccagatagg aagcctcgcc tctggcgata tcctcaatga
      421 ggttgataag ccttggggga ttcaatgatt gcgcaaactg tgccgaacgg gcgatcgtcc
      481 aaaccgagag agtgcttgaa gtctctgttt gaagcagctg ctggtctgga tacgatcgag
      541 aagggaagcc gacccagccg ttaccatcct gtcggtacaa gcccgcctcc tcgacagaga
      601 gccctgtggg caacgcatcg agcgaatact taacctgaac ttgggcgggg gggagattgt
      661 gagcgttctg atagcccatg ttctggatga ccgtccatcg ctctgaagcc ggcacattgt
      721 ctgcaatgcc aggcgttaca aacaattcag gcgcgctggc gcttctccac gcagcggcgt
      781 attgctgtcc tctgtgttcc gtgaacctga gagcaacacc tgagttgcca agatttgtcc
      841 agcccgtgag atccgtgttg accacacctg tgaaccggtt tgcaggcacg ggagccgtcg
      901 tgatcaccag tcctgaagag gacatcccat agactatccc ggaacgggtt gtgcagagat
      961 cctcgaagac atcggagagg ttctccagtt cgactgttgc tgtgcctgcg agaaacttgt
     1021 agattcggct gcccccttga tagagagtgc ttaccgcata ggcagcatcg gccgtcgccg
     1081 tcatggccac cagactgtac tcagaacggt tttcgacacc gttgatgctt ctccaggcat
     1141 tacccgattt gtagatagca atactctggt ctccaacagc gtagacgtag ttgccatagc
     1201 cggatattgc acgaatctgc gacgttgttc ctgttgattc cactgaccat gcactgccat
     1261 tgtattttct gatctgaccg ttattcagac cgacatagat ttcggagtcg ctctttcccc
     1321 aaaggcacgt gagccagacg ttctcgggta gatcttcgaa cagc
//
LOCUS       DAOQUC010001294         1363 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_136645_length_1363_cov_3.396789, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001294 DAOQUC010000000
VERSION     DAOQUC010001294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1363
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_136645_length_1363_cov_3.396789"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /gene="hslV"
                     /locus_tag="PKH07_17695"
     CDS             <1..330
                     /gene="hslV"
                     /locus_tag="PKH07_17695"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019867896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HNT36827.1"
                     /translation="KGNLKRAAVELAKDWRTDKILRHLEALLAAADRESSLLVSGSGE
                     VMEPDDGILAIGSGGPYALAAARALLDREDLSVKQIVERALQIAAGICIYTNEEIRVE
                     SLDDEIE"
     gene            354..488
                     /locus_tag="PKH07_17700"
     CDS             354..488
                     /locus_tag="PKH07_17700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36828.1"
                     /translation="MTADQNKPTQEEEFGWKFHRFPLIVSIVLTALFYIAIFIYVAME
                     "
     gene            607..>1363
                     /locus_tag="PKH07_17705"
     CDS             607..>1363
                     /locus_tag="PKH07_17705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36829.1"
                     /translation="MRVCTLFFSILGVFAMMAMHCSAEQGAARSEKTLAVYGVNLGKP
                     PLEGKTSEEAAGFLGDLGVNAVFGGYDSAEFVAACRSRGITVYASLGIFVGENYWNSH
                     PESHPVDALGNPIEKVQWYAGVCPNQPWLRAEKLTAIAKKAEEGLADGVWLDFIRYPV
                     HWEDGAPRPKQDETCFCPVCLAKFQKDTGLTFPEQIHSASETAEYLLTTRREEWFDWR
                     AEQIAEFVKEASATLHRANPKAKLGLFCVPWQPQ"
BASE COUNT          306 a          323 c          420 g          314 t
ORIGIN      
        1 aagggcaacc tgaagcgtgc cgcggtcgaa ctggcaaagg actggcgcac ggacaaaatc
       61 cttcgccatc tggaagcgct tctggcggcc gcggatcggg aatcatccct tctggtttcg
      121 ggaagcggtg aagtgatgga gcccgatgat ggcatccttg ccattggatc aggtggcccc
      181 tatgcgttgg ccgcggctcg ggcgctcttg gatcgagagg atctctcagt caaacagatt
      241 gttgaacgtg ccttgcagat cgctgcggga atctgtatct ataccaatga agagattcga
      301 gtggaatcac tcgacgacga gatcgagtga atcggaaggt ggttgagaag acgatgacgg
      361 ctgatcagaa caaaccaaca caagaagagg aatttggctg gaagtttcac cgttttcccc
      421 tgattgtatc catcgtgttg acggcgctgt tctacatcgc cattttcatc tatgtggcga
      481 tggagtagcg gaactccttg cggggcaagc gaatgccgca gatggataat acgatcaggc
      541 tgttgttgcc agattgcgac ctgggagaga cgtgaaggga ttcggttccg caggtaagtg
      601 cctactatga gagtttgcac attgtttttc tcgatactgg gggtttttgc catgatggca
      661 atgcattgct ctgctgaaca gggcgctgcg agatcagaaa agacgctggc ggtctatggg
      721 gtgaaccttg gcaaaccgcc tttggaaggc aagacgtctg aagaggctgc tggctttctt
      781 ggggatctcg gagtcaatgc agtttttggc ggctatgaca gcgccgagtt tgtcgccgcg
      841 tgcagatcaa gggggatcac cgtctacgcc agcctgggaa tctttgtcgg agagaactac
      901 tggaactctc atcccgagag ccatcccgtt gatgcattgg gcaatccgat tgaaaaagtg
      961 cagtggtatg cgggggtctg ccccaaccaa ccctggttac gagctgagaa actcactgcg
     1021 attgccaaga aagcggaaga aggtctggcg gatggtgttt ggctggattt catccgctat
     1081 ccggtgcatt gggaagatgg tgcgcctcgt cccaaacagg acgagacgtg cttctgtcct
     1141 gtgtgtcttg ccaagttcca gaaggatacc ggcctgacct ttccggagca gattcattca
     1201 gccagcgaaa ctgcggaata cctgcttacg acacggcgcg aggaatggtt cgattggcgt
     1261 gctgagcaga ttgcggagtt tgtaaaagaa gccagcgcca ccctgcatcg tgccaatccg
     1321 aaggcgaaac ttggactgtt ctgtgttccc tggcagccgc agg
//
LOCUS       DAOQUC010001295         1362 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_136810_length_1362_cov_2.794185, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001295 DAOQUC010000000
VERSION     DAOQUC010001295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1362)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1362)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1362
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_136810_length_1362_cov_2.794185"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1033)
                     /locus_tag="PKH07_17710"
     CDS             complement(<1..1033)
                     /locus_tag="PKH07_17710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36830.1"
                     /translation="MHLNRYGFQRPTYTPLPTPGYTPAVIIPEPSTPVPQGCPLAYLG
                     NTGTSNPHLHFEIRDQSTFTPTATQTMSPTSTYSPTVTPTQEPGSPTWTAAPTSSPTP
                     TNSPTPVPTVPSGTALPYDYMNVFDYLTVDNTQSCRTILESALVIPETGSKLEPISPL
                     DAVKYAPGLPGCLLLNLNGTTPLRFRLIGRCKFVIRAYDRITPDGNKLGLYWIGYRKE
                     GDPLLNYELEFDKVLYDERRDRLGDIYRLDQKFGTTLNPTQYWYKMYYERTATPTPFN
                     LKSHIDYGVFDSAMYEVGPEIRLLLEAQQYPYTGSPPLPVRQRLLVVHVDSDVSWVDC
                     DEGSDDNPGT"
BASE COUNT          312 a          319 c          386 g          345 t
ORIGIN      
        1 aggtcccagg attgtcatcg gacccttcgt cacaatcgac ccaagacacg tcactatcaa
       61 cgtgcacgac gagaagcctc tggcgcaccg gcaagggtgg agatccagta tagggatact
      121 gctgtgcctc gagcagcaga cgtatttcgg ggccaacctc gtacatcgcg ctgtcgaata
      181 cgccataatc aatgtgggac ttgagattga atggcgttgg cgtggcagtc cgctcgtagt
      241 acattttgta ccaatattgt gtcggattga gagtggtccc aaatttctga tcgagcctgt
      301 agatatcccc aagcctatca cgtcgttcgt catatagaac cttgtcgaac tccagttcat
      361 aatttagcag ggggtcgcct tccttacggt agccgatcca gtagagaccc agtttgtttc
      421 catctggcgt gatccggtca tacgcccgga ttacgaattt gcaccttcct atcagacgga
      481 aacgcaacgg ggttgtacca tttagattca gcaagaggca gcccggcagg cccggtgcat
      541 acttgacggc gtccagcgga ctgatcggct caagcttgga tccagtctcg ggaatgacga
      601 gcgccgattc aaggatcgtg cggcaggatt gagtgttatc gactgtaaga taatcgaata
      661 cattcatgta gtcgtagggt agtgctgttc cagaaggcac agtcggaaca ggtgttgggc
      721 tgttcgttgg agtcggagag gaagtcggcg cagcagtcca ggtcggagat cctggttcct
      781 gggttggtgt gaccgtcggt gagtatgtcg aagttggtga cattgtttgc gttgctgtcg
      841 gagtgaaagt actttggtca cgaatctcaa aatggaggtg cgggttgctc gttcctgtgt
      901 ttcccaggta ggcgagcgga caaccttggg gaaccggcgt gctcggctct gggatgataa
      961 ctgccggtgt atatcctgga gtaggcaacg gtgtatatgt gggcctttgg aagccatatc
     1021 tgtttagatg catataaaca gtcaccactt cgtcgttatg aatgatctcg tatgtgttac
     1081 ccccagaacc gagttggact ctcttttcat gtacggttcc gctatccgcg gcctgaacta
     1141 tagtatagcc gtcttgtcct ggaagatcga ttccaccatg gaaatggccg ccgctactgg
     1201 ggcggaattc tcccagagtt cctgcaatat ggtcaggttc tactggccat tgagcggtta
     1261 tccgctcttg ggacgaacaa gtatgcgaga ctgggtgaag tatgaaaaac agacaagaaa
     1321 acaaaggtaa aagtcgtttc atggcttcac atcctcaact at
//
LOCUS       DAOQUC010001296         1361 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_136925_length_1361_cov_3.691424, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001296 DAOQUC010000000
VERSION     DAOQUC010001296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1361
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_136925_length_1361_cov_3.691424"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1307)
                     /locus_tag="PKH07_17715"
     CDS             complement(<1..1307)
                     /locus_tag="PKH07_17715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNT36831.1"
                     /translation="MAVRKADQELLALVQRGLSQITDEERQRIDEEWFGVGWAESRQK
                     PAHLIWLFVFAIALVSVAMTAAFGRRWLKDLPNITAARDSLLNLWLVFLSLISPLLLI
                     ASLARALEFPWTSLQTTQICIAVILWATAIFRHRIAYSVRVFLLLGMFFLLGTGGLLT
                     YGLLGTTTITFFVFVIVATTLFGVRGSIFSITLSLLSIGSVGVAVGAGFWGYSIDTEA
                     YSVSAMLWTLTFIGFGLWSVVATLGIGEVYRHMEDALTRQRESEEHYRTLVQSANSII
                     LRLNHNGQITFFNEYAQKFFGFSEQEILGRDALGTIIPETESTGRDLRTMMDDLLAHP
                     DLYVLNRNENVRRNGERVWVSWTNRAFLDERGELDEVLCIGSDVTALKAAEDALRESE
                     SFFRGVFENSMAGIMTMDRKGIVRTANPRFAEMFGYSQHEIVG"
BASE COUNT          352 a          388 c          326 g          295 t
ORIGIN      
        1 ctgccaacaa tctcgtgctg tgaataaccg aacatttcag caaaccttgg gttggctgtt
       61 ctgacgattc cctttctatc catcgtcatt atgcctgcca tcgagttttc gaacacgccg
      121 cggaagaaac tttcactttc ccgaagggcg tcttctgcag ccttcagtgc agtcacatca
      181 cttccgatgc agagcacttc atccagctct cccctctcgt ccaaaaaggc tctgtttgtc
      241 caactgaccc aaaccctctc gccgttgcgt cgaacgttct cgttgcggtt gagaacatag
      301 agatctggat gtgccagaag gtcatccatc atagttctga gatcgcgtcc cgtgctctcc
      361 gtttcaggaa tgatggttcc gagcgcatct cttccgagga tttcctgctc actaaaaccg
      421 aagaactttt gcgcatactc attgaagaaa gtgatctgcc cgttgtgatt gagtcgcaaa
      481 atgatgctat tcgcactctg aacgagtgtg cggtagtgtt cttcactctc tctctgcctt
      541 gtgagtgcat cttccatatg tctgtagact tcaccgatac cgagcgttgc aacaacagac
      601 cacagaccaa agccgatgaa ggtgagcgtc caaagcattg ccgatacgga gtaagcctcc
      661 gtatcgatcg agtagcccca gaaccccgct ccaacagcca cacccacact gccgatagaa
      721 agcagactca gcgtgatgga gaaaatgctg ccacggactc cgaagagcgt ggtagcaacg
      781 atgacaaaga cgaaaaacgt tatcgttgtc gttcccagca gcccgtaggt cagaagtccc
      841 cctgttccga gcaggaagaa catcccgagc agcaggaaga ctcggacaga ataagctatc
      901 cgatgacgaa aaatggcagt tgcccacaga atcacagcga tacagatttg cgtcgtctga
      961 agagaagtcc aaggaaactc cagggctctc gccagagacg caatcagtaa aagtggggag
     1021 atcaaggaga ggaaaaccag ccagaggttg agcaaactat ccctggccgc cgtgatgttc
     1081 ggcaggtctt tgagccacct tctgccaaag gcagcagtca ttgcaacgga caccagcgcg
     1141 atcgcgaaaa caaataacca aatgagatga gcaggcttct ggcggctttc cgcccagccg
     1201 acaccaaacc attcctcatc gatcctctgt ctctcttcat cggtgatctg agagaggcct
     1261 ctctgcacca gcgcgagaag ttcctggtct gccttacgaa ccgccatccg aaggtctgtt
     1321 cgaaagaaag ggcgacccga gcgaagaaac cccttggctc c
//
LOCUS       DAOQUC010001297         1361 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_136928_length_1361_cov_3.571975, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001297 DAOQUC010000000
VERSION     DAOQUC010001297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1361
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_136928_length_1361_cov_3.571975"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(11..622)
                     /locus_tag="PKH07_17720"
     CDS             complement(11..622)
                     /locus_tag="PKH07_17720"
                     /inference="COORDINATES: protein motif:HMM:NF014025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HNT36832.1"
                     /translation="MDFNLLLENALYFLALINPASKIFLLSSIEPRLTGRELRALSLR
                     ATVVAFVILCCLSTLGNYLLHDIFRVEIYSLRVAGGIVLFLIGLTAVTKGRFYERSLQ
                     HSADISIVPLGAPLIAGPGTIAASVAFSSEHGVLLAITGFSLALILNLVLMLFSLQIG
                     ALIEKLNATGPLIRITGLIVATVAVQMVLTGLGEWISVVRVPM"
     gene            complement(625..>1361)
                     /locus_tag="PKH07_17725"
     CDS             complement(625..>1361)
                     /locus_tag="PKH07_17725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNT36833.1"
                     /translation="RWGQLTGMRLAFGVALFMAMLAAIMQQILIEDDRAGRKSGDESK
                     AEKNPLRLYREMSPGLRNLLVSDILIRFCEQIPYAYVVLWCLQRIISPVDEFQFGVLS
                     AVEMVTAMLVYIPVAYLADRGTKKPFVLMTFMFFTLFPLVLLLTQSFWFLVGAFVLRG
                     LKEFGEPTRKALIMDLAPEGKKAAMFGLYYLMRDVVVSIAAFGGAYLWMISPQVNFLT
                     AFGCGVAGTLWFALRGVDMPQRREVN"
BASE COUNT          392 a          391 c          336 g          242 t
ORIGIN      
        1 aaggcacaga tcacatgggc acgcgaacca cgctgatcca ctcccccagt cccgtcagta
       61 ccatctgtac cgcgacggtc gcaacgatga gaccagtgat tcggatgagc ggacctgtcg
      121 cattgagttt ctcgatcaat gcgccgatct gaagtgagaa caacatcaga acaagattca
      181 gaatcagtgc cagcgagaat ccagtgatgg caagcaagac accgtgctcg gaactgaaag
      241 caacagatgc ggcaatcgtt ccagggccgg caatgagcgg tgcgcccaga gggacgattg
      301 aaatgtccgc ggaatgctgc agactgcgct catagaagcg tcccttggtc accgctgtca
      361 gtccgatgag aaagagaacg atgccaccag cgactcggag tgagtagatc tccactcgaa
      421 agatatcatg caagagataa ttccccagag tactcaggca acagaggatg acgaaagcca
      481 cgacggtcgc tcgaagagac aaagcacgca attcccgccc tgtgagacgg ggctctatcg
      541 aagacagcag gaatatcttg ctggcagggt tgatcagagc cagaaaatag agtgcgtttt
      601 ccagcaacag gttgaaatcc atgtctagtt cacttccctg cgttggggca tatccacgcc
      661 tcgcaacgca aaccaaagcg ttccggcaac accgcaacca aaagcagtca ggaaattcac
      721 ctgaggactg atcatccaca gataagcgcc cccaaacgct gcaattgaga ccacaacatc
      781 ccgcatcaga tagtacaatc cgaacatcgc ggccttcttg ccttccggcg cgagatccat
      841 gatgagagcc ttccgagtcg gctctccaaa ctccttcagg cctctgagaa caaaagcacc
      901 taccaggaac cagaaagact gtgtcaacag aagaaccaac ggaaagagcg tgaagaacat
      961 gaaagtcatc agaacgaagg gtttcttcgt tccccgatct gccagatagg caacaggaat
     1021 gtagaccagc atcgccgtga ccatctccac cgcagacaga acgccaaact gaaattcatc
     1081 cactggcgag atgatgcgct gcaggcacca gagaaccaca tacgcgtatg gaatctgctc
     1141 acagaaccga atcaggatgt ctgataccaa cagattccga aggcctgggc tcatttccct
     1201 gtagagacgc aatggattct tctccgcctt ggactcgtca ccggactttc gacctgccct
     1261 gtcgtcttcg atcaagatct gctgcatgat ggcagcgagc atcgccataa acaacgccac
     1321 gccaaaggca agtctcatgc cggtcaactg cccccatcga a
//
LOCUS       DAOQUC010001298         1361 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_136976_length_1361_cov_2.575804, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001298 DAOQUC010000000
VERSION     DAOQUC010001298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1361
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_136976_length_1361_cov_2.575804"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..410
                     /locus_tag="PKH07_17730"
     CDS             <1..410
                     /locus_tag="PKH07_17730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36834.1"
                     /translation="KTCEDTILTLMEEVEQETRHLKLREQEILGEIKELDAKSAELSR
                     SLEQKKTELVQAETAAQEARNNVHNTLIGTFDKLYARNRGKAVVPVFGNSCSHCNVSV
                     TLQTLAQLQDGWDIIRCEGCSCILYMDKTEEAV"
     gene            complement(451..756)
                     /locus_tag="PKH07_17735"
     CDS             complement(451..756)
                     /locus_tag="PKH07_17735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013032508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative addiction module antidote protein"
                     /protein_id="HNT36835.1"
                     /translation="MKKKTSTSRYDVAEHLRTPEEMAAYLEACLEESEGDAAFVAKAL
                     GDIARAKGMAQVAREAGLSRESLYKALSGERTPTFDTILKVVHALGIQLHAAPLRSG"
     gene            complement(761..1057)
                     /locus_tag="PKH07_17740"
     CDS             complement(761..1057)
                     /locus_tag="PKH07_17740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007308199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HNT36836.1"
                     /translation="MTDIRKTDIFAKWIDGLKDIRARARIQARIERLALGNFGDVKPV
                     GKGVAELRVDYDPGYRVYFKRSGRTVIILLAGGDKRTQSRDIDTALRLARELED"
     gene            complement(1162..>1361)
                     /locus_tag="PKH07_17745"
     CDS             complement(1162..>1361)
                     /locus_tag="PKH07_17745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36837.1"
                     /translation="KCQEKFRQRSFDRRFNRMDLETDRTISTRRQLMGLATHPTVFCC
                     NSRRLWIGASALLDTPCTVSL"
BASE COUNT          328 a          375 c          351 g          307 t
ORIGIN      
        1 tcaagacctg tgaggacaca attctcacgc tcatggaaga agtggaacag gaaaccagac
       61 acctcaaact gcgagagcag gaaatccttg gcgaaatcaa agaactggat gccaagagcg
      121 ctgagctgag ccgctccctt gagcagaaga aaaccgaact ggtgcaagcg gaaacggcag
      181 cacaggaagc gcgcaacaac gttcacaaca cgctcatcgg cactttcgac aagctgtatg
      241 cccgtaatcg aggaaaggca gtggtgcctg tttttggcaa ttcatgcagc cactgcaatg
      301 tcagcgtcac actgcagact ttggcgcagc tgcaggatgg ctgggacatc atccgctgcg
      361 aaggctgctc ctgcattctc tacatggata aaaccgaaga agcggtttag cctgaagtgg
      421 gtctggtgac ccatgctaac acaatcctga ttatccgctg cgaagaggtg ccgcgtgcag
      481 ttggatgcca agtgcatgta ccaccttgag gattgtatcg aaagttgggg ttcgttcgcc
      541 ggagagcgcc ttgtagagac tctcccgaga aagtccggcc tcacgagcaa cttgtgccat
      601 gcccttggca cgagcgatat ccccgagcgc cttggcaaca aatgccgcat ctccttctga
      661 ctcttcaaga cacgcttcaa gataggcagc catttcctct ggagtcctca agtgctctgc
      721 tacatcgtag cgacttgtgc tcgtcttctt cttcattggt tcagtcctcc aactcacgcg
      781 cgaggcgcag cgccgtgtca atgtcccggc tttgggtacg tttgtcacca ccagccagaa
      841 ggatgatgac ggtacgacca gacctcttga aatagacccg atagccgggg tcgtagtcaa
      901 ccctcaactc agcgactcct ttgccaaccg gtttgacatc accgaagtta ccaagggcaa
      961 gcctttcgat tctggcctga atgcgagcgc gtgcccggat atccttcagt ccgtcgatcc
     1021 atttggcgaa gatgtccgtc ttccgaatgt ccgtcattat ctgcactgta gccaaaagga
     1081 tacagcacgt caagatgaat cctcttctgc tgggtactga ctcaggacgt taggatgggt
     1141 cgccagaccc atcagttgtc ttcagagtga caccgtacag ggtgtgtcca gcaaagcgga
     1201 cgcaccaatc cacagtcttc gcgaattgca gcagaagacg gtaggatggg tcgccagacc
     1261 catcagttgt cttcttgttg agatggttcg atcagtttcc agatccatcc ggttaaaacg
     1321 tctgtcgaaa cttcgctgcc gaaatttctc ttgacatttc t
//
LOCUS       DAOQUC010001299         1361 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_136983_length_1361_cov_2.534456, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001299 DAOQUC010000000
VERSION     DAOQUC010001299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1361
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_136983_length_1361_cov_2.534456"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="PKH07_17750"
     CDS             <1..181
                     /locus_tag="PKH07_17750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36838.1"
                     /translation="GATEHSLNFQRTEFMLVLIQDLRCSGRGIGQTKGTNRFSAQHLK
                     LPGMWAERLPSVEEI"
     gene            complement(549..878)
                     /locus_tag="PKH07_17755"
     CDS             complement(549..878)
                     /locus_tag="PKH07_17755"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HNT36839.1"
                     /translation="MTAQEVQDTIEKMAQRIAQQFDPDLIVLFGSYAKGIATLDSDLD
                     ILVVLPVEGSVRQTACAIDALLSDRVLPVDLIVLTPEQFERQKDRSGTIACEAVREGK
                     IIYERAA"
BASE COUNT          326 a          340 c          362 g          333 t
ORIGIN      
        1 tggcgcaacc gaacactcat tgaatttcca aagaacggag ttcatgttgg ttttgatcca
       61 agaccttcgc tgctcaggaa gaggaatcgg gcaaacgaag ggcacaaatc gcttcagcgc
      121 tcagcatctc aaacttcctg ggatgtgggc ggaaaggctt ccctcggtag aagagatctg
      181 acggcttcga taacgcgcat cgctgaccga agggagtctt cggcttcact ttgcgaaatg
      241 ggctcccatt cgccggggta tcgtgcttct actgaataac ggttgagttg tctcaggtcg
      301 tccctgagac tctcgagact tttgaacgca gcgcagtcga gtcggttcgc caagagtatc
      361 aggtcatgag ttctggggaa tggcttttgc agaaaagtga gaagcgcctt taggtatttc
      421 tctgcgacct gctgacagtg aaagcaaagg tttcggtcgg gcaggcgttc tccatagtca
      481 ggacatattc cgcattgcgc cggtcgttct ctgctttctc tatccatctc cggacttctt
      541 ggagacactc aggctgctcg ctcatagatg atctttcctt cccgaactgc ttcgcaggcg
      601 atggttccag aacgatcttt ttgccgttcg aactgctcgg gagtcagtac tatcagatcc
      661 acggggagaa cgcgatctga tagaagggca tcaatggcgc aggcagtttg acgcacggag
      721 ccttccacgg gcaagacaac caggatatcg agatcactgt cgagcgtagc aatacccttc
      781 gcatatgagc caaagagaac aatcaggtca ggatcgaact gctgtgcaat ccgctgtgcc
      841 attttctcaa tggtatcctg aacttcctga gcagtcatga tggacttcct ttctgtttcc
      901 tttctccctg cttattattg cttactgtat cttcgagagc aagaggcatc aagacctctg
      961 ttcgatttta ccgagcagga tatcgcgata gattccttgc ggcaagcaca cacccttctc
     1021 cggtgcaaac ggacactcat tatttgccag cctctcccga aaatcccccc agcccccctt
     1081 gttaaggggg gttccggaga atacccccct taacacaact acccattacc cacaagtagt
     1141 gggagtgtaa gaaggcacag tgaaaagagt atgggaaaga gacttgtaga gcaaggcgtg
     1201 gagcaaagaa cctgtttttg ggggaaaatg caggggacga tagagcgctg gagcctttca
     1261 cagtcaaaga gagtctgcat ttgtcggaag aaggaagttt gttggtgggg gaaccgtcat
     1321 tccctgagcg cttggatggt ggcgacaatg atctccaggt c
//
LOCUS       DAOQUC010001300         1360 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_137098_length_1360_cov_3.707280, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001300 DAOQUC010000000
VERSION     DAOQUC010001300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1360
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_137098_length_1360_cov_3.707280"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..148
                     /locus_tag="PKH07_17760"
     CDS             <1..148
                     /locus_tag="PKH07_17760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013277133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="twitching motility protein PilT"
                     /protein_id="HNT36840.1"
                     /translation="GIGYLDDGTMVVVDDGIQYLSQKTSVQVTSVLQTAAGRMIFAKP
                     VIGA"
     gene            153..629
                     /gene="ispF"
                     /locus_tag="PKH07_17765"
     CDS             153..629
                     /gene="ispF"
                     /locus_tag="PKH07_17765"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HNT36841.1"
                     /translation="MYRVGCGYDIHRLGEARPLILGGVHLEYPRGLVGHSDADVLIHA
                     IMDALLGAAGLGDIGQHFPPGDPAFLNADSRGLLRHVICIVSEAGFEVGNVDAVVVAE
                     EPKISPHIASMKANLAADMGIAESRIGIKATTNEGLGAEGRKEGISAHAVALLKEV"
     gene            632..>1360
                     /locus_tag="PKH07_17770"
     CDS             632..>1360
                     /locus_tag="PKH07_17770"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HNT36842.1"
                     /translation="MLEIKRISQLSKQERRAILNRSSARMAETMERIRPIVEKVAQEG
                     DSAVIEYTERFDGVRLKPEEFFVPQEQIEQAWGETDDELKIALSLCHSNIRNFHRDIK
                     THLASGWAKTYSTGSEKDRWVCGIMPTAVDRAAGYVPGGTAAYPSTALMVSVPAFAAG
                     VGDFVITTPPREAGTVPQAVLAAAKIAGVTRILRVGGAQAIAALAFGTETIPQVDVVV
                     GPGNVFVTAAKAYLSALGVVGIDFP"
BASE COUNT          297 a          356 c          416 g          291 t
ORIGIN      
        1 ggggatcggc tatctggatg acggcaccat ggtcgttgtg gatgacggca ttcagtacct
       61 gagccagaag accagcgttc aggtcaccag tgtgctgcaa accgcggcag gcaggatgat
      121 ttttgccaaa ccggtgatcg gagcgtaaaa gagtgtaccg tgttggctgt ggttatgata
      181 tccatcgcct gggagaagcg cgtccgctga tcctgggcgg tgttcatttg gagtatccgc
      241 gtggcctggt aggtcactcg gatgcggatg tgctcattca cgcgatcatg gatgcccttc
      301 tgggggcggc aggacttgga gatatcggac agcattttcc acctggagat cccgcctttc
      361 tgaacgcgga cagccgcggg ctgctgcgtc atgtgatatg catcgtatca gaggccggat
      421 tcgaggttgg aaacgttgat gcggtcgtgg ttgccgagga gcccaagatt tcgccacaca
      481 tcgcttcgat gaaggccaac cttgccgccg acatgggcat agcggaaagc cgcatcggga
      541 tcaaggccac caccaacgaa ggacttggtg cagaaggcag aaaagaaggc atctctgcac
      601 acgctgtggc actactgaaa gaggtctaga catgttggag atcaagcgga tttcccaact
      661 atcgaaacag gaacgcagag cgattctcaa tcggtcgtct gcacgtatgg cggaaacaat
      721 ggagcggatt cgccccatcg tagaaaaggt cgcccaggaa ggcgactcag ctgtcatcga
      781 gtacaccgaa cgttttgacg gtgttcgact gaaaccggaa gagtttttcg ttccgcaaga
      841 acagatcgaa caagcctggg gagagaccga tgacgaattg aagatcgccc tgagtctctg
      901 ccactcaaac atccgcaact tccatcgcga cattaagacg catctggctt ccggctgggc
      961 gaaaacctac tccaccggat cggaaaagga tcgctgggtt tgcggaatca tgcccaccgc
     1021 ggtagaccgt gcagcgggat acgttcctgg cggaaccgcc gcgtatccat ccacggcgct
     1081 gatggtttcc gtgccggcat tcgcagcagg tgtaggtgat tttgtcatca caacacctcc
     1141 ccgcgaggca ggcactgtac cccaagctgt tcttgcagca gcgaagattg cgggcgtgac
     1201 tcggattctc cgcgtcggtg gcgctcaggc gattgctgcc cttgcgtttg ggactgagac
     1261 gattccgcaa gttgacgtcg ttgtcgggcc tggcaatgtt ttcgttaccg cggcaaaggc
     1321 ctatttgagc gccctgggtg tggtcgggat agatttccct
//
LOCUS       DAOQUC010001301         1360 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_137108_length_1360_cov_3.349425, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001301 DAOQUC010000000
VERSION     DAOQUC010001301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1360
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_137108_length_1360_cov_3.349425"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            124..1098
                     /gene="trpS"
                     /locus_tag="PKH07_17775"
     CDS             124..1098
                     /gene="trpS"
                     /locus_tag="PKH07_17775"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013112579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HNT36843.1"
                     /translation="MKTLFSGIQPSGDIHIGNYLGAIRNWVRLLDKYQCIYCIVDYHA
                     ITIEYDVQEMLSRIIGAATVNIAAGLDPEKCILFRQSDVVQHTELAWVLNTVTPMGDL
                     ERMTQFKDKSRQHASNVNAGLFTYPVLMAADIMLYKAEAVPVGDDQIQHLELCREITR
                     KFNNRFGEVFPEAQPLLSESPRILGLDGKSKMSKSLGNTISLVDSPELIREKLATAVT
                     DENRKRRKDPGNPDVCNLFTMHKGFSTAEQLKWVDEGCRSASIGCLECKKLLADNMLA
                     DIAPIQERIAEFAKKPDLVRQILDEGARKCRVIAEKTMDEVHGVMGFK"
     gene            1115..>1360
                     /locus_tag="PKH07_17780"
     CDS             1115..>1360
                     /locus_tag="PKH07_17780"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNT36844.1"
                     /translation="MATESAKTVIDAIKCRRSIRHYKADALPKAAIEHLLDAARYAPT
                     ALGKQTCNFLVVTNKTVLKQVGAILQGRLEFGLRWRPI"
BASE COUNT          321 a          340 c          372 g          327 t
ORIGIN      
        1 ggaactgcat gcgcagcatg aaggtctctt gagacgtctg gatgttatcg gttagaatct
       61 gacggcagat ttctgtcgtc gtgatgcttt ttttatgcga cacgggacgg aggaatttgg
      121 ctcatgaaga cgctcttcag cgggattcag ccaagcggcg atattcacat cgggaattat
      181 cttggtgcca tccgcaactg ggttcgtctg ctcgacaagt atcagtgcat ttactgcatc
      241 gttgattatc acgccatcac cattgagtat gatgttcaag agatgctgtc tcggatcatc
      301 ggtgctgcca cggtcaatat cgcggctgga ctcgatccgg agaagtgtat tctctttcgg
      361 caatctgatg tggtacagca tactgaactg gcctgggttc tcaacaccgt gactccaatg
      421 ggtgacttgg aacgcatgac tcagttcaag gacaagtctc ggcaacatgc ttccaacgtg
      481 aatgcagggc tcttcactta tcctgttctg atggcagcgg acatcatgct ctacaaggca
      541 gaagccgtgc cagttggtga cgaccagatc cagcacctgg aactctgtcg ggagattacc
      601 cgaaagttca ataatcggtt tggcgaggta tttcctgagg cgcagccgtt gctttctgaa
      661 tctcctcgta ttctcggttt ggacgggaag tccaaaatga gcaaaagttt gggcaacaca
      721 atctcactgg ttgactctcc tgaattgatt cgagagaaac tggccacggc ggtcactgat
      781 gagaaccgca agcgccgcaa ggatccgggc aaccctgatg tgtgcaacct cttcacgatg
      841 cacaagggct tctccaccgc agaacaactg aagtgggtgg acgagggctg ccgttccgcg
      901 agcatcggct gtctggaatg caagaaactt cttgccgaca atatgcttgc cgacattgct
      961 ccgatccagg aacgcattgc tgagtttgcc aagaaacctg atcttgtgcg gcagattctc
     1021 gacgaaggcg ctcgaaagtg cagagtcatt gctgagaaga ccatggatga agttcacggt
     1081 gtgatgggtt tcaaatagcc aggaggctca gaccatggca acggaatcgg ccaagacagt
     1141 gatagatgcc atcaagtgtc ggcgctcaat tcggcactac aaagccgacg ctctgcccaa
     1201 agccgcgatc gagcacttgc tcgatgctgc ccgatacgct ccgacagctt tgggaaagca
     1261 gacctgcaac tttcttgttg ttaccaacaa gaccgttttg aagcaggtgg gcgccatcct
     1321 tcagggcagg cttgaattcg gtttgcgttg gcgaccgatc
//
LOCUS       DAOQUC010001302         1360 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_137158_length_1360_cov_2.550958, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001302 DAOQUC010000000
VERSION     DAOQUC010001302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1360
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_137158_length_1360_cov_2.550958"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..551)
                     /locus_tag="PKH07_17785"
     CDS             complement(<1..551)
                     /locus_tag="PKH07_17785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36845.1"
                     /translation="MRRRHAIGIINLALIGLATTPVVAESLQLVEMRLIEMSTRVKTV
                     KASMRIEDRLQIGNQLLPRRRTGTYEGARLNGITKSRETLEIVIGGGGRWTPPRNPAD
                     KGAKKKDDYLDYGYTPAKSDFALGYLLNGAINTVMGRDEEASDNYKEAATLNEDLVLL
                     IERLRSKRYNTLLIVDWGSGPEK"
     gene            complement(619..>1360)
                     /locus_tag="PKH07_17790"
     CDS             complement(619..>1360)
                     /locus_tag="PKH07_17790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36846.1"
                     /translation="ADGLPDSAEPTLNEVYEILRTQGLNEDKTVEAVVINEGVKRWKG
                     EPFEQALMYCYIGIQKAMRGEWGNARAAVAGSVFMLKDFAENTNEKDRRGSGLTTMEL
                     ATRACVAQVPEKNFGTATLIYNGDACVNVHEGPGGRRVTPSGRDAIDHVVGDEEFFLK
                     LHSDYDVSLLRNRTVNGEKAFVIQATVRKDKPAAGNRIVFYFSQATGVLLRRVIYMCD
                     GQVYNNTVFTDHQVNVEIPANRFDVDTK"
BASE COUNT          270 a          414 c          320 g          356 t
ORIGIN      
        1 atcttctccg gtccactgcc ccagtcaacg atcaagagag tgttatatcg tttgctccgc
       61 aatcgctcga tcagcagcac aagatcttcg ttgagagtgg cagcctcctt gtagttgtcg
      121 ctggcctctt catcacgacc catgacggtg ttgatggcgc cgttcaacag ataccccaat
      181 gcgaaatcgc tcttggccgg tgtgtagccg tagtccaggt agtcgtcctt cttcttcgct
      241 cctttgtcag cggggttccg cggcggtgtc catctgccac cccctcctat aacaatctca
      301 agtgtttcgc gcgacttggt tatcccgttc aaccgcgcac cctcataggt gcctgtacgc
      361 cgtcgtggca gcaactgatt tccaatctgc aggcgatcct caatcctcat gcttgctttg
      421 acagtcttga cccgtgtact catctcgatc agtcgcattt caaccagctg cagtgattca
      481 gccacaaccg gagttgtagc cagcccgatg agcgccagat tgataatgcc aattgcatgc
      541 ctgcgtcgca tggttgtctc ccggtaaggc tctctatgaa gccgcatccg cctgtcataa
      601 cggcaaccga ttctcacttc acttagtgtc cacgtcgaag cgatttgcgg gaatctccac
      661 atttacctga tggtcggtga acacggtatt gttataaacc tgaccatcac acatgtagat
      721 cacccgtctc agcaagacac ctgtcgcttg gctgaaatag aagacgatcc tattcccggc
      781 tgcgggtttg tcttttcgca cggttgcctg aatgacaaag gccttctctc cgtttacggt
      841 acgatttcgc agcaaactga catcgtagtc actatgtagt ttcagaaaga actcttcatc
      901 tccgacgaca tggtcgatcg cgtcacggcc cgatggcgtc acgcgtcttc caccaggccc
      961 ttcgtggacg ttcacgcacg catcgccgtt gtatattagg gtggcagttc cgaagttctt
     1021 ttcgggaacc tgagcaacgc acgcccgagt agcgagttcc attgtggtca gcccactgcc
     1081 cctgcggtcc ttctcgttgg tattctctgc aaaatccttt agcataaaaa cagaacctgc
     1141 cactgcggcc cgcgcattac cccattcgcc ccgcatcgcc ttttggatcc caatgtagca
     1201 atacatcaag gcctgctcga agggctcacc cttccatcgc ttcacgcctt cgttgatgac
     1261 gactgcttcg acggtcttgt cctcgttcag accctgggtt ctcaggattt catagacttc
     1321 gttgagcgtg ggctctgcgg aatctggaag gccatcggcc
//
LOCUS       DAOQUC010001303         1359 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_137289_length_1359_cov_3.080521, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001303 DAOQUC010000000
VERSION     DAOQUC010001303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1359
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_137289_length_1359_cov_3.080521"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="PKH07_17795"
     CDS             <1..195
                     /locus_tag="PKH07_17795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36847.1"
                     /translation="LLDQLIAREESKGVGGPALENARAARAAAESMIKSLTEYEQSGG
                     RYVDVRDEIAEAIEGLIAQE"
     gene            complement(530..>1359)
                     /locus_tag="PKH07_17800"
     CDS             complement(530..>1359)
                     /locus_tag="PKH07_17800"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNT36848.1"
                     /translation="VEGEIAISGDTERTLLARGTLLQDASGRTVGAVVVFSDVTRLRR
                     LENMRRDFVANVSHELRTPVTSIKGFVETLLDGAINDRDDAQRFLGIIARHADRLNAI
                     IEDLLTLSRIEQEVRRAEIEREVCRLRPILASVIEVCSLRAAEKSIRTELSCEDTLEC
                     KMNAPLVEQAVINLVDNAIKYSPSEATVQIAAQQTNDACTISVEDSGCGIPQEHLPRL
                     FERFYRVDKARSRNLGGTGLGLAIVKHIVQAHQGTVSVVSEMGKGSRFTIHIPKSLD"
BASE COUNT          310 a          378 c          370 g          301 t
ORIGIN      
        1 ctgctcgatc agctcatcgc ccgcgaggaa agcaaaggcg tcggtggccc cgcgctggag
       61 aacgcccgag ccgcgcgcgc agcagccgaa tcaatgatca agtcgctgac cgaatacgaa
      121 caatcaggcg gacgatatgt ggatgtccga gacgagatcg ccgaggccat cgaggggttg
      181 atcgcccaag agtagcccct cccgaagaca agcgctcatg tgcaataagg tttccggtta
      241 caaagtgagt aaagtctacg tacgcttggt ttccgtgctt gttggaaagt ctgattcgat
      301 aaatggtgat gcgttcggac agtctcttcg tgtggctcga gtcaagccaa gaacctgccc
      361 tcccttgtca gcgcagagat cccggccacc acggaataca tggaagacca acaaggccag
      421 caggctgcgg caaagaggcg gagttggtat gctgagattg gccacttgtc cagaaatgct
      481 tttccgaact gtagaggcga tgtgtggagg aagtccttcc gttcgccgtc taatcgaggg
      541 acttcggaat atgaatcgta aaacggctgc cttttcccat ctcacttact acggaaaccg
      601 ttccctgatg cgcttgcacg atatgcttca caatagccaa accaagcccg gttccgccga
      661 gattcctgct tcgggcttta tccacgcgat agaaacgctc gaacagtcgt ggcaaatgct
      721 cctgagggat cccgcatccg gagtcttcca cagagatcgt gcaggcgtcg ttggtctgct
      781 gagcggcaat ttggactgtt gcctctgatg gactgtattt tatggcattg tccacgaggt
      841 tgataacggc ttgctccacc aatggagcgt tcattttgca ttcgagggtg tcttcacagc
      901 tgagttcggt gcgaatactc ttctctgctg ctctgaggct gcagacctca atcacggaag
      961 caagaatcgg tcgcagtcgg cagacttccc gctctatctc agcccgcctg acttcctgtt
     1021 cgatgcgtga aagcgtgagc aagtcctcga tgattgcatt gagtcgatcc gcgtgccgag
     1081 cgatgatccc caggaaacgc tgagcatcgt cgcgatcgtt gatcgctcca tccaggaggg
     1141 tctcaacaaa ccccttgata gaagtaacag gagttcgaag ttcatgagaa acatttgcca
     1201 caaaatcccg cctcatattc tccaagcggc gaagtcgggt gacatcgctg aagaccacca
     1261 ccgcgcccac ggttctacca gaggcgtcct gaaggagcgt tccacgcgcc agcagggttc
     1321 tttcggtgtc tccactgatg gcaatctcac cctcgacag
//
LOCUS       DAOQUC010001304         1355 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_137922_length_1355_cov_3.357692, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001304 DAOQUC010000000
VERSION     DAOQUC010001304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_137922_length_1355_cov_3.357692"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="PKH07_17805"
     CDS             <1..313
                     /locus_tag="PKH07_17805"
                     /inference="COORDINATES: protein motif:HMM:NF013228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Rid family hydrolase"
                     /protein_id="HNT36849.1"
                     /translation="FREIQISGTAAIDEAGHSLYPGDFKKQVVRTFDNVEALLAQGAA
                     TLQDFCSGTAFIKRVGDSEEYGNILEELDLKSLPCVWVAADICRDDLLFEIDGIAAVA
                     K"
     gene            complement(332..811)
                     /locus_tag="PKH07_17810"
     CDS             complement(332..811)
                     /locus_tag="PKH07_17810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36850.1"
                     /translation="MLKKRWLALAVCLGLLAISCIYVRPPRLSVEERQKVAQRLQATT
                     VPSAKYSSAAILLLLNEPQDHDLVAEQLDRETDPALLASLIIALAHWGQQESAEQVVK
                     HLTDTRPVTCLLIDGKEGTTTVSEIAAKAMHALKRPRPRRNLPRPFPFAVKTDLIVK"
     gene            complement(827..>1355)
                     /locus_tag="PKH07_17815"
     CDS             complement(827..>1355)
                     /locus_tag="PKH07_17815"
                     /inference="COORDINATES: protein motif:HMM:NF017743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HNT36851.1"
                     /translation="ATSFLQVNPAIAARLYEAVDMIETSNRRLALDGYCGVGILTLML
                     AEKFESVIGLDSDRKAIRYAIQAAREQSVQNVSFYDEPLPRGLGKGQCDLASQSMVVL
                     DPPRGGCDEETLHRITLSGTDTIAMISCHPATLARDLSRLDAFEIEEVLPFDMFPQTY
                     HIESLVVLRRRNRST"
BASE COUNT          319 a          343 c          349 g          344 t
ORIGIN      
        1 cttcagagaa attcagattt ccggtactgc ggcgattgat gaggcaggac acagcctcta
       61 cccaggagac tttaaaaaac aggttgtaag gacttttgat aacgttgagg cacttctggc
      121 gcagggggcg gcaacgctcc aggatttctg ttctgggact gcttttatca agcgtgttgg
      181 agactctgag gagtacggta acatattgga agaattggat ttgaagagcc tcccttgtgt
      241 ttgggtggct gctgacattt gccgcgatga tcttcttttt gagattgatg gtattgcggc
      301 cgtggccaag tgaacggttt ctggcgtaga actatttcac gatcaggtca gtcttgacgg
      361 caaaaggaaa agggcgggga agattccttc tgggtctcgg cctcttgagg gcatgcattg
      421 ccttagcggc aatctccgaa acggtggtag tcccctcctt gccatcaatg agcaggcacg
      481 tcacaggtct ggtatcagta agatgtttca caacctgttc cgcggattcc tgctgtcccc
      541 agtgagcgag agcaataatg agcgaggcga gaagtgccgg atccgtctcc cgatccaact
      601 gttcggcaac cagatcgtga tcctgcggtt cgttcagcag caacagaatt gctgctgaac
      661 tatactttgc cgaaggcaca gtggttgcct gaagacgttg tgccaccttc tgtctttcct
      721 caacagaaag ccggggtgga cgtacataga tacatgatat cgccagtaac cccaggcaga
      781 ccgccagagc aagccatctc ttcttcagca ccaaaagcct ccgtcatcac gtacttctgt
      841 ttcgacggcg tagcaccacc aaagattcaa tatgatatgt ctggggaaac atgtcaaatg
      901 gcagaacctc ttcaatctca aaggcgtcga gtcgtgagag atcgcgcgca agagttgctg
      961 gatgacagga aatcatggct atcgtatccg ttccgctcag ggtgattcga tgcagagtct
     1021 cttcgtcaca tccccctctc ggcggatcca ggactaccat actttgactg gcaaggtcac
     1081 attgcccttt tccaagtccc ctcggcagag gttcgtcata gaaagagaca ttctgaacgg
     1141 attgctctct cgcggcttga atcgcatatc gaattgcctt ccgatctgag tccaagccga
     1201 ttacggactc aaatttctcg gcaagcatca acgtcaatat gcccacaccg cagtacccat
     1261 ctagtgccaa tcttctgttg cttgtttcga tcatatccac agcttcgtag agtcttgcag
     1321 cgatggctgg atttacctgg aggaaacttg tcgct
//
LOCUS       DAOQUC010001305         1355 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_138020_length_1355_cov_1.937692, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001305 DAOQUC010000000
VERSION     DAOQUC010001305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_138020_length_1355_cov_1.937692"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="PKH07_17820"
     CDS             <1..295
                     /locus_tag="PKH07_17820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007806967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36852.1"
                     /translation="QSNIAISGDAPSVYLKRIEDKQGISKQDLDDILRSHLIEPDYLR
                     SDDFDAFFSSRTKSLSSLITEAMGKPVVEEQGTNEEESEDSTKDSEIDEDGES"
     gene            365..919
                     /locus_tag="PKH07_17825"
     CDS             365..919
                     /locus_tag="PKH07_17825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36853.1"
                     /translation="MTDFQEEEEGVPLGFVSSPRHGQHFDLAKLAVRKLKSANLALKG
                     KNEREFEQAVVGHLQSSPTIRKNLITQVGTDEVDKITQASLFGFSHRPDASIGKDGTA
                     IEIKVISGGQSAREILGQSIAYRMQYRFVIIVLIDRSEGRQIVDLCSDKKSSEFSLFA
                     GLAESMNIFSVIGPDGPSSNIAFI"
     gene            1106..1324
                     /locus_tag="PKH07_17830"
     CDS             1106..1324
                     /locus_tag="PKH07_17830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36854.1"
                     /translation="MHITMEIAEPFERKGDDVIVNITKRIIDAGASPRTEHLFLRLKN
                     HADASDKEIEQLAWSAIMEHLEKIPEDR"
BASE COUNT          396 a          296 c          359 g          304 t
ORIGIN      
        1 acaatcaaac atcgcgatta gtggtgacgc tccgtccgtt tacctgaaga gaatagagga
       61 caaacaaggc atttcaaaac aagacttgga tgacattctg cgtagccact tgatcgaacc
      121 cgattatttg cgttcagatg atttcgatgc ctttttttca tcacgtacca agtctctttc
      181 atcccttatc acagaggcta tgggtaagcc agtcgtggag gagcagggta caaatgaaga
      241 ggaatccgaa gatagtacta aggactcaga gattgacgaa gatggagagt cctgattaca
      301 gctgctttct tgcggggggc gggccattgt ggcaagctgg tttttcctat aacgcagtgc
      361 tggaatgacg gatttccagg aagaggagga aggtgtgccc ctaggatttg tgagtagtcc
      421 gagacatggc caacattttg atctggcaaa acttgccgtc agaaaactga agagtgcgaa
      481 tcttgcgctg aagggaaaaa atgagaggga attcgaacaa gcagttgttg gtcacctgca
      541 gtcatcgcca accattcgaa agaatctaat cactcaggtg gggacagatg aggtcgacaa
      601 aataactcaa gccagcttgt ttggcttttc ccacagacct gatgcttcga ttggaaaaga
      661 cgggaccgcc attgagatca aggtgatatc gggtggacaa tcggctaggg agatcttggg
      721 tcagtcaatt gcctatcgca tgcaatatag gttcgttatc attgtgttga tagacagatc
      781 cgaaggcaga caaattgtag atctctgcag cgacaagaag agcagcgagt tctcattgtt
      841 cgcaggacta gccgaatcca tgaacatatt ttccgtgata ggcccggacg gaccttcaag
      901 caacattgcc tttatttgag gggcgacccc ggggcacttt cccactctgt cgataaaggc
      961 caagagactg atggcaggag aaccgatggg aagggaagag aactcgcgac ccggggcata
     1021 cgacccggcc cagacgatgg cggttacttc aggtcaagta tgttacctcg gcactaaacg
     1081 gtgctgacca ggaggtgctt caatagtgca tataacaatg gaaattgctg aaccattcga
     1141 acggaaaggc gatgatgtaa tcgtaaacat caccaagagg ataatcgatg caggagcatc
     1201 ccccaggact gaacatttgt tcctccgcct aaagaaccac gccgacgcgt ctgacaagga
     1261 gattgaacaa ctcgcttggt cagcaataat ggaacacctt gaaaagatcc cagaggacag
     1321 atgagttcag taaaccttcg ccaagagaat agaat
//
LOCUS       DAOQUC010001306         1354 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_138157_length_1354_cov_2.280216, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001306 DAOQUC010000000
VERSION     DAOQUC010001306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1354
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_138157_length_1354_cov_2.280216"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1245
                     /locus_tag="PKH07_17835"
     CDS             <1..1245
                     /locus_tag="PKH07_17835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36855.1"
                     /translation="SPAMVFYSRCYFQEMSLVFFTFATIALGWRFHRHRIATWAILSG
                     ICAGLMFVTKETCIIAYSAILLGTAINILWYRWHKKIDDWPVGHLSTWPVLVGTVAGV
                     VFSALFFSSFAKHPWGIVDSVKAYQTYCYRGYCGGDHVQPWTYYLQMLLLWEVGRGPV
                     WSEGLVLALAAVGVALSFRKRLPDGDPIFLRFLAFYAILMVAFYSAIPYKTPWCMLSF
                     HHALILLAGFGASALLRMTRQRLSKVVVFCLLLLGTGHLGWQSYLASYRFSTDTRNPY
                     VYAHTVPSLLKLVDRVQAVVDVSPKREDTVILVVSQDYWPLPWYFRGFRHVGYWENIR
                     DREYPESPLIITSVAFSHVVEQKFGTGYKSEFYGLRPDVMLVLHIRDDLWDAFMETRR
                     QVLPGAISWDICPPSHTQAEDS"
BASE COUNT          245 a          346 c          365 g          398 t
ORIGIN      
        1 tcaccggcaa tggtttttta cagccgctgc tactttcagg agatgagtct ggttttcttt
       61 acattcgcca ccattgccct tggatggcgg ttccataggc atcgaatcgc cacctgggcg
      121 atcctttcgg gcatctgcgc cggtctgatg tttgtgacca aggaaacctg catcattgcc
      181 tactcggcca tcctgttggg gacggcgata aatatccttt ggtatcgttg gcacaaaaag
      241 attgacgatt ggccggtcgg ccatttgagc acctggcctg tgcttgttgg gacggtggca
      301 ggagttgttt tctccgctct tttcttctcc tcctttgcga agcatccatg ggggattgta
      361 gattcagtca aagcctatca gacctactgc tacagaggat actgtggggg cgatcatgtc
      421 caaccgtgga cttactatct tcagatgctt cttttatggg aagtgggcag agggcctgta
      481 tggagtgaag gccttgttct cgcgcttgct gcagttggcg ttgctctcag tttcagaaaa
      541 cgcttgcctg atggtgatcc tattttcctg cgtttcctcg cgttctacgc gattctgatg
      601 gtggctttct actcggcgat tccgtacaag actccctggt gtatgctgag tttccatcat
      661 gcgctgatcc tgcttgcggg ttttggtgcc agcgcactct tgaggatgac ccggcagcgg
      721 ctgtccaagg ttgttgtgtt ctgtttgctg ctgttgggca ctggccatct tggctggcag
      781 tcatatctgg cttcctatcg tttctctaca gacacgcgca atccctatgt ctatgctcac
      841 accgttccca gtctgctgaa acttgttgat cgagttcagg ccgttgtgga tgtgtcgccc
      901 aaacgagagg atacggtaat actcgttgtt tctcaggact actggccttt gccttggtac
      961 ttccgaggat ttcggcatgt tgggtactgg gagaatattc gcgatcgaga gtatcccgaa
     1021 tcccctctga tcatcacatc agttgctttc tctcatgttg tcgagcagaa gttcggcacc
     1081 ggttacaaga gcgagttcta cggcttgaga ccagatgtca tgctggtgct tcacatccga
     1141 gatgacctct gggatgcttt tatggaaacc aggaggcagg ttcttcctgg ggcgatctca
     1201 tgggatatct gtcctccatc ccatacgcag gcggaggact cctgacatga agatcgatct
     1261 tcagggtgag gcagggattc cccgggacat ctgtctcgac gtgacatcat ctcatccaac
     1321 tgcctctctg atgcggtttg cctgcgacgc catg
//
LOCUS       DAOQUC010001307         1351 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_138583_length_1351_cov_4.165895, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001307 DAOQUC010000000
VERSION     DAOQUC010001307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1351
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_138583_length_1351_cov_4.165895"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..341)
                     /gene="mutL"
                     /locus_tag="PKH07_17840"
     CDS             complement(<1..341)
                     /gene="mutL"
                     /locus_tag="PKH07_17840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015376389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HNT36856.1"
                     /translation="MPLITVLPPDVASKIAAGEVVQRPASVVKEMVDNALDAKASRVQ
                     IEIDEGGKSCIAVIDNGVGMGADDVRVCVERHATSKIRTAEDLDSVITLGFRGEALAA
                     ISSVARFTLTPR"
     gene            complement(359..>1351)
                     /locus_tag="PKH07_17845"
     CDS             complement(359..>1351)
                     /locus_tag="PKH07_17845"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNT36857.1"
                     /translation="VGTDKGRSNRAGWIWGGCFLLGVVLLVGCADMHLYTKDELVGIS
                     LQEARKAKRLEEKGKNDEALEHYKRSLTISERPAVHCDMGELLSKMNRFDEAQEHFYA
                     ALASSPDFPRAKRGLERLDARKKLQALGKTPEEISAAMKRWDEEYVPSGEGAVARPDA
                     SETYREYLVRQGVAVDEVSVKLYERGVELARQGKTDQAIRMFREVLMENPNDARVYYN
                     LGNLYYQNDDLRRSMMQYQTAIECDKQYAAAYNNLGVVQEALGYSDYALDNYVAAIAI
                     GDYEPAHYNLAVLYTRFGKVEEAIKEFETYVNISPQGQFVESAKEQLGKLYRIR"
BASE COUNT          278 a          401 c          338 g          334 t
ORIGIN      
        1 cggggggtca acgtgaatcg ggcgacagat gaaatggcgg cgagtgcctc gcctcgaaac
       61 ccgagcgtga tcaccgaatc gagatcttcc gccgtgcgaa tcttgcttgt tgcatgtcgt
      121 tcgacacaga cgcgaacgtc gtcggctccc atgccgacac cgttgtctat gacagcgata
      181 caggactttc ccccttcgtc aatctcgatt tgtacccgcg aggccttggc gtcgagggcg
      241 ttgtcaacca tctccttgac gaccgaagcg gggcgctgca ccacctcgcc tgcagcaatt
      301 tttgaagcga catccggtgg cagaaccgta atcagaggca ttctggctcc gtgatctctc
      361 agcgaatccg atagagcttg cccaactgct ctttggcgga ttcgacaaac tgcccctgcg
      421 gactgatatt gacataggtt tcgaattctt tgatggcttc ttcgactttg ccgaaccgag
      481 tgtagagtac cgcgaggtta tagtgagccg gttcatagtc accaattgcg atcgcggcca
      541 cataattgtc cagagcatag tcggaataac ccagtgcttc ttgaacgact cccaagttgt
      601 tgtaagccgc tgcatactgc ttgtcgcatt cgatggcggt ctgatactgc atcatggatc
      661 ttctgagatc gtcattctga tagtacagat tgccgagatt gtaatagacg cgggcgtcgt
      721 ttgggttttc catcaaaact tcgcgaaaca tccgaattgc ctgatcggtc ttgccctgtc
      781 ttgccagttc gacaccgcgt tcatagagtt tgacactgac ttcatcaacg gccacccctt
      841 gtcggacaag atattcccga tatgtttcgc ttgcatctgg ccgtgccaca gcgccctcgc
      901 ccgatggaac atattcctca tcccatcttt tcatggcagc ggaaatctct tcgggagttt
      961 tcccaagcgc ctgaagcttc ttgcgggcat ccaggcgctc cagtcctctc ttcgcgcggg
     1021 ggaagtccgg ggatgaggcc agcgcggcgt agaaatgctc ctgagcctca tcgaaacgat
     1081 tcatcttgct gagcagttcg cccatgtcac aatgaaccgc aggacgctca ctgatggtca
     1141 gagatcgctt gtagtgctcc agcgcctcgt cattctttcc cttttcctca agcctcttgg
     1201 ccttgcgcgc ttcctggagc gaaatgccga ccagctcatc cttggtgtac aggtgcatat
     1261 cggcgcaccc gacaagcaac acgactccca gcaagaaaca ccctccccag atccatcctg
     1321 cgcgattgct tctaccctta tctgtgccaa c
//
LOCUS       DAOQUC010001308         1351 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_138592_length_1351_cov_3.627315, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001308 DAOQUC010000000
VERSION     DAOQUC010001308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1351
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_138592_length_1351_cov_3.627315"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..412)
                     /locus_tag="PKH07_17850"
     CDS             complement(<1..412)
                     /locus_tag="PKH07_17850"
                     /inference="COORDINATES: protein motif:HMM:NF012974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase N-terminal domain-containing
                     protein"
                     /protein_id="HNT36858.1"
                     /translation="MAEKTLPHNLDAERAVLGAMLRDTTALGTAFEILGQNSFYHEGH
                     LKIYLALQALYMRGQPTDLYSVAEELRQLGDLDKAGGTAYIASLLNSVATSANLEYHA
                     EIVRDKSVLRDLIQSCINTAEEGFQEHGSSSDLLE"
     gene            complement(848..1300)
                     /gene="rplI"
                     /locus_tag="PKH07_17855"
     CDS             complement(848..1300)
                     /gene="rplI"
                     /locus_tag="PKH07_17855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HNT36859.1"
                     /translation="MKVILRQDVDKLGAEGKVVDVKDGYACNFLIPRGMAVEATTQRV
                     KNINHERKLIEDRKRRETKEATTLAKKIEQVSCNIPVQVGEEDKIFGTVTTADIAASL
                     AEKGITVDRKKITLDEPIRALGVYNAKIKIAHEVTANLRVWVEKRRDD"
BASE COUNT          307 a          394 c          315 g          335 t
ORIGIN      
        1 gctccaggag atctgaggaa cttccgtgtt cttggaaacc ctcttcagcg gtgttgatgc
       61 aactctggat gagatcgcgc agaacagatt tgtcccgaac gatttccgcg tgatattcca
      121 gattcgctga agtagccaca ctgttgagaa gcgaggcgat gtaagcggtt cctcctgcct
      181 tatccagatc cccgagctgg cgaagttcct cggccaccga gtacaagtca gtgggttgac
      241 ctcgcatgta caacgcctgc agggcaagat agatcttgag atgaccttcg tgatagaaac
      301 tgttctgtcc cagaatctca aatgcggtgc ccagcgctgt ggtatccctg agcatggcgc
      361 caagcacagc acgttccgca tcgagattgt gtgggagtgt cttctctgcc atccctagtc
      421 catcctatca aacaaatatg cccgagatct ctagcaccga gctgaatcca ttcgaagcgc
      481 gtacgcaaga cctatcacag agacaatgct cacttgaacc aaacccgtct cgttgcgagc
      541 cgtgtctctg agtatgaatc agccaagacg atggagaaaa ccatgagcaa acctcggata
      601 actctctcgc cgttaccttc gcaaatccgg actccaagaa agtccggctt ctggttcgag
      661 aaggcttatg gggatgtggg catagccctc gatactgccc gatcctcaaa gagaaagcat
      721 cggcacaatc atgcccttgg tttgtctgaa caatgacgct tggatttcca gaatcccctt
      781 ccgtccacaa gaccttcgag cctccggatc cgcactccaa aaccaacgtc tgccaagagg
      841 cgtcagttca gtcgtcgcgt ctcttttcaa cccagactct gagatttgca gtcacttcgt
      901 gggcaatctt gatcttggca ttgtagacac ccagggctct tatgggttca tccagtgtga
      961 tcttcttgcg atccacggtg atccccttct ctgcaaggga agccgcgatg tccgcagtgg
     1021 tgaccgtgcc aaaaatcttg tcctcttcac cgacctgcac cggtatgttg caggacacct
     1081 gctcaatctt ctttgcaaga gttgtcgctt ccttggtctc gcgccgctta cggtcttcaa
     1141 tgagtttgcg ctcgtggttg atgttcttga cccgctgagt ggtggcttca accgccatac
     1201 ctctcggaat caggaaatta cacgcataac cgtccttgac atcaacaact ttgccctcag
     1261 ccccaagctt gtccacatcc tgtctgagaa tgaccttcac ccgtctgctc tccttactcc
     1321 actaattctg attacgaaag acctcgacca g
//
LOCUS       DAOQUC010001309         1351 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_138594_length_1351_cov_3.608025, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001309 DAOQUC010000000
VERSION     DAOQUC010001309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1351
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_138594_length_1351_cov_3.608025"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(141..809)
                     /locus_tag="PKH07_17860"
     CDS             complement(141..809)
                     /locus_tag="PKH07_17860"
                     /inference="COORDINATES: protein motif:HMM:NF014093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033178 - proton-transporting two-sector
                     ATPase complex, catalytic domain [Evidence IEA];
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E family protein"
                     /protein_id="HNT36860.1"
                     /translation="MSNSEQLAEVPQPAAVFEQQISQEADRERASILERARRTAEEIR
                     NKASQQVQQSLDAALQKARKQADVQREHLLSKTRLEIRRERLLVQESVFLKALAKVHE
                     HFETKRGSEQHKDLLVGLTLEAVYALRDSDIQIGIAARDREFFEVRCLPEILRRVQKS
                     RMDTLNVVVENLESVDARLSEDTIGVLVRSRDGRLEYDNTVGARMRRMDETLRAIAFE
                     VLLA"
     gene            complement(796..>1037)
                     /locus_tag="PKH07_17865"
     CDS             complement(796..>1037)
                     /locus_tag="PKH07_17865"
                     /inference="COORDINATES: protein motif:HMM:NF014092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HNT36861.1"
                     /translation="QTREDLVAHCQALDRVLREGQAGLIFITEKLAMQIREEIETLVL
                     GGKFPLVLEIPDRKGSLGQRTRIREIVGRAIGVQF"
     assembly_gap    1038..1047
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1139..>1351)
                     /locus_tag="PKH07_17870"
     CDS             complement(1139..>1351)
                     /locus_tag="PKH07_17870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit C"
                     /protein_id="HNT36862.1"
                     /translation="IVGWAFLSAAICTGLGCVGAGIAVAYVGAAALGLVGERPELLGR
                     ALIFVGLAEGIAIYGVLMAFYIVSRI"
BASE COUNT          300 a          416 c          321 g          304 t
ORIGIN      
        1 agctcctgaa gcgcgtcacg ttcgcgagcc agctcgacca ggtaatcctc cataaatccc
       61 cagtcgatct tctcacggcg tttcaggact ttactgataa aatcagtctt cggcacgttg
      121 gcgctcccaa agtcttgttg ctatgcgagc agaacctcaa acgcgatggc gcgcagtgtc
      181 tcgtccatac gcctcattcg ggcgccaact gtattgtcat attccagacg accatccctg
      241 gagcgcacca gcacgcctat cgtgtcttcc gacagtcgag catcaacact ttccagattc
      301 tcgacaacca cgttaagggt atccattctg cttttctgca ccctgcgcag aatctctggc
      361 aagcagcgga cttcaaaaaa ctcgcgatct cttgcggcga tgccgatttg aatatcgctg
      421 tcacgcaagg cgtatactgc ttccaaggtg agcccaacga gcaagtcctt gtgctgctca
      481 gagccgcgtt tggtttcgaa atgctcatgc actttggcaa gagccttcag gaaaacactt
      541 tcctggacga gcagacgctc acggcggatc tccaaacggg tctttgaaag cagatgctct
      601 cgctggacat ctgcttgttt gcgcgctttc tgaagtgcgg catcgagcga ttgctgaacc
      661 tgctgagacg ctttgttgcg aatctcttcg gccgttcggc gggcgcgttc cagaatggac
      721 gctctctccc tgtctgcttc ctgactgatc tgttgttcaa acacggcagc aggctgcggt
      781 acttcggcga gttgttcaga attggacacc gattgccctc cctacgattt cccgaatcct
      841 ggttctttga cccaaagacc ctttgcggtc ggggatttcc aacaccagtg ggaatttgcc
      901 ccccaagacc agagtttcta tctcttctcg aatctgcatc gcgagtttct cagtgatgaa
      961 gatcagtccc gcttggcctt cacgcagaac ccgatcaagc gcctgacaat gagccacaag
     1021 atcttcacgg gtttgcgnnn nnnnnnncat cccgcgtgcc agcaacactt cccggcacac
     1081 cgacgaggga aaagccgatg actgtctcct catctccgat aacgtgcaga ttcatggatc
     1141 agatcctgct gacgatgtaa aaagccatca gcactccata gatggcaata ccttctgcca
     1201 aaccgacaaa gatcagggcg cgtcccagca gctccggtcg ctcgccaacc agacccagtg
     1261 cggcagcgcc gacatacgcc acagcaatac ccgcacccac gcagcccaga ccggtacaga
     1321 ttgcggcgct cagaaatgcc cagccaacga t
//
LOCUS       DAOQUC010001310         1351 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_138638_length_1351_cov_2.680556, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001310 DAOQUC010000000
VERSION     DAOQUC010001310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1351
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_138638_length_1351_cov_2.680556"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..400)
                     /locus_tag="PKH07_17875"
     CDS             complement(<1..400)
                     /locus_tag="PKH07_17875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36863.1"
                     /translation="MRKAPQIAIVLLTLFVRSAVSQEPPSVIADGTWESLTLRVYSSV
                     AAINSGTLEMYGGYKADRISIDATSRIAVYAVSDSFQRSSEPSGSGGAYGGRGGGQFG
                     SATLRSAPDSLEISSDYYGALPWGTGGDRGK"
     gene            complement(469..>740)
                     /locus_tag="PKH07_17880"
     CDS             complement(469..>740)
                     /locus_tag="PKH07_17880"
                     /inference="COORDINATES: protein motif:HMM:NF017020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HNT36864.1"
                     /translation="AVLFSRGIEFKRTHDLAELTALLNHKGVETPIAETLLLCLNPYA
                     VIFRYDEIDVELVSLQDAFKWTVDIRTWAEQLVSKAEAEDDLRSR"
     assembly_gap    741..750
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(873..1193)
                     /locus_tag="PKH07_17885"
     CDS             complement(873..1193)
                     /locus_tag="PKH07_17885"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HNT36865.1"
                     /translation="MIEEQTLQQAVARIVAVAQPCRVILFGSYGRGDADEQSDVDLMV
                     VKPRVDNRGEEMIRLAQAVGRIGAGVDILVYSDEEYQRRSQVPGTALYWARKEGRVLY
                     ESPH"
BASE COUNT          315 a          390 c          305 g          331 t
ORIGIN      
        1 tcttaccacg gtcacccccc gttccccagg gtagggcacc gtagtagtca gaactaatct
       61 ccagggaatc aggtgcagag cgaagtgtcg cactgccgaa ttgcccccct cctctgccac
      121 catacgctcc cccactgccg cttggttcac tagatctctg gaaactgtcg ctaacggcat
      181 atacagcaat tcttgaggtg gcatcaatcg agatccgatc agccttgtaa cctccataca
      241 tttccagtgt ccccgagttg attgccgcaa cagatgaata cacccgaagc gttagcgatt
      301 cccaagttcc gtctgcgatt acgcttggcg gttcctgtga aacggcactt ctgacaaaga
      361 gagtcagcag aacaatggcg atttgcggcg cttttctcat agcattacct ccagattacc
      421 tttttccttg tggcacttgg cgcaagagaa atcgcccatt gagccattct atcggcttct
      481 gagatcgtcc tctgcttccg ccttgcttac cagttgttca gcccacgtcc tgatatcaac
      541 tgtccatttg aatgcatctt gaaggctgac cagttcaacg tctatctcgt cgtaacggaa
      601 aattactgcg tagggattca ggcacaacaa cagagtctcg gcaatcggag tttctacccc
      661 tttgtgattc agcaatgctg tcagttccgc caaatcgtgt gttctcttga actcaatccc
      721 acgactgaaa agaaccgctt nnnnnnnnnn atttctcaat ggcttgttga acatggaaga
      781 agatcactga ggggtggctg tcaggatcac cgttcaagac tttgaacgca tgtatgtcgc
      841 ggtcggcaag acgcagacaa cgacgcgcct cctcaatgtg gggactcata caacactctc
      901 ccctctttgc gcgcccaata taaagctgtc cccggaacct ggctgcgtcg ctggtattcc
      961 tcgtctgagt agacaagaat atccacaccc gcaccgatgc gacctaccgc ctgtgccaga
     1021 cgaatcatct cctcgcctcg gttgtccaca cgcggtttga caaccatcag atccacatcg
     1081 gattgttcat cggcatcacc ccgtccgtac gaaccgaaga ggatgacgcg acagggttgt
     1141 gccacggcta cgatacgcgc cacagcttgc tgtaatgtct gctcttcgat catatcatca
     1201 aactccgcct ggtttcgaga ctgtcttacg cttgcatctc acttatagcc tattctcaac
     1261 gaaacttgca agagagaatc gcgttgcaga gcatcagctc agttatgcag gtgatacagg
     1321 caaggtatgc ttcggaagca aagcggagtc t
//
LOCUS       DAOQUC010001311         1350 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_138759_length_1350_cov_3.743629, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001311 DAOQUC010000000
VERSION     DAOQUC010001311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1350
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_138759_length_1350_cov_3.743629"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1350)
                     /locus_tag="PKH07_17890"
     CDS             complement(<1..>1350)
                     /locus_tag="PKH07_17890"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNT36866.1"
                     /translation="PDPSADRVLIEAVQNSSGMMRIGMINSLANRKAKDATSLIASLL
                     KNEDMQTAEAAAFALGCIGGEQGVEAVSAALRDAQGSLRLACSQALLQCADQFAAQGQ
                     DDKAKEIYTRMYAKESEPVIRARAFGSLLSMTKDLNLMMQALGDADPKVQGRASQIGV
                     EMPGSKATARLSALLGKSTPETRSLLLRVLGDRGDRTALKKVRRWSQDENPVVRLAAL
                     EAVGKLGDAGCVPLFAKRLASADVDERNVIRKSLDGLSGDRVNRAIIRQLPKSKPKAQ
                     VELIQSLTVRDATESASLLLKMTDSPDRKVRVEAFKALSELGSTDDIPALSERVLHCD
                     ESDSKEAESALVRLVRRLGHQSSIGAVLQERFIREQEAGVRVSLIRLIGAMGDPGSLD
                     TLRDATKDRDESVRYEAIRALAAWPTSDPIDTLLELAKSADKETHQVIALRGFIDQI"
BASE COUNT          283 a          396 c          356 g          315 t
ORIGIN      
        1 cgatttggtc aatgaatcca cgaagtgcga ttacctggtg cgtttccttg tcggccgact
       61 tcgccaactc gagcagagta tcaatcggat ctgacgttgg ccatgctgcc aatgcgcgaa
      121 tcgcttcata gcgcactgat tcgtccctgt cttttgtggc atcgcgcagc gtatccaacg
      181 agccaggatc acccatcgca ccaataagcc gaatcaggga tacacgcaca ccggcttcct
      241 gttctcgtat gaagcgttcc tggagaactg ctccaatcga ggactgatgc ccgagacggc
      301 gaaccaacct gacaagggca ctctccgctt ctttgctatc ggattcatcg cagtgaagta
      361 ccctctccga aagtgctgga atgtcatccg tcgagccgag ttccgagagg gctttgaacg
      421 cttcgactcg gacttttcga tccggactgt ccgtcatctt tagcagaagc gacgcagact
      481 ctgtggcatc acgcaccgtg aggctctgga tcaactcaac ttgtgccttt ggcttactct
      541 tgggcaactg gcggatgatg gcgcggttga cccgatcgcc cgaaagaccg tcaaggctct
      601 tcctgatgac atttcgttca tccacatcgg cactggcaag cctcttggca aatagcggca
      661 cgcaaccggc atcaccgagt tttccaactg cctcaagagc ggccagacgt acgacggggt
      721 tctcgtcctg actccacctt cttacctttt tcagtgctgt tcggtcacca cgatctccca
      781 ggacgcggag cagcaatgag cgcgtttccg gagtggattt cccaagcagc gcagacaatc
      841 gggcagtggc cttcgaaccg ggcatttcga ctcctatctg tgacgcgcgg ccttgaacct
      901 ttggatctgc atctccaaga gcctgcatca tgaggttgag atccttggtc atggaaagaa
      961 gagaaccgaa tgcgcgtgcc cgaataacag gttcgctctc cttggcgtac attcttgtgt
     1021 aaatctcctt tgccttgtcg tcctgacctt gggctgcaaa ctggtcggcg cactggagca
     1081 aagcctgaga acaggcgaga cgcaatgatc cctgcgcatc tcgaagtgcg gcggagacag
     1141 cctcaactcc ttgttcccca ccgatgcatc ccaaggcaaa agcggcggcc tctgctgtct
     1201 gcatatcctc attcttaagt agcgaggcga tcaacgaggt ggcgtctttg gcttttctgt
     1261 tggccagtga gttgatcatc ccaatccgca tcatgcctga agagttctga acggcctcga
     1321 tgagcacccg atcggccgag gggtctggga
//
LOCUS       DAOQUC010001312         1347 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_139278_length_1347_cov_2.691950, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001312 DAOQUC010000000
VERSION     DAOQUC010001312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1347
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_139278_length_1347_cov_2.691950"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="PKH07_17895"
     CDS             <1..320
                     /locus_tag="PKH07_17895"
                     /inference="COORDINATES: protein motif:HMM:NF012693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36867.1"
                     /translation="SRCYLFENNRLVSMTCDDTLAGHGLTRVAGTKRLFFQRKDIELS
                     RHQLLLLTSDGIHGYMTDQEIGAICRKHSKQPADVVRSLIQASLQAGGMDNATAVAIL
                     INK"
     gene            complement(323..886)
                     /locus_tag="PKH07_17900"
     CDS             complement(323..886)
                     /locus_tag="PKH07_17900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36868.1"
                     /translation="MSITHLDPEPWRSLLESSTAGNIRIRFSSVGIPKPSEVNKGGIN
                     CLYIQRKAANIRSEVALWKEVFTALKDTAFPDSLFQDKAYPLRRVFFPSMPVYAPLVL
                     QYCYKLIADRESTLQRDTLTRALKMDSESQLIDVLKTELDDPSLPVSVVNVVKSIFGE
                     GKVPETDNAEEAKIYLKREIQSRTNGS"
     gene            complement(1057..>1347)
                     /locus_tag="PKH07_17905"
     CDS             complement(1057..>1347)
                     /locus_tag="PKH07_17905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36869.1"
                     /translation="MTDSTSEKYELGILLRSTDSSGSPYQRYGLRDKYTTKKKNGNLT
                     GVSLALDDFLYLRTRGISDCTPGYDEWSSLFEGTGNSALTHEFLECGVRIKW"
BASE COUNT          351 a          334 c          289 g          373 t
ORIGIN      
        1 attcgcgatg ctacctattt gagaacaacc gcctcgtctc tatgacctgt gacgatacac
       61 ttgccggcca tggactgacc cgggttgcgg gtacaaagag gttgttcttt cagagaaaag
      121 acatcgaact ctcaagacac caacttctcc ttctcaccag cgatggaata catggataca
      181 tgacagatca ggaaattgga gcaatctgta gaaaacacag caaacaacct gctgatgtag
      241 tccgttcatt gatccaagcc tccctgcaag cgggaggaat ggacaacgcc actgcggtag
      301 cgatcctaat caacaagtag aatcaactgc cattcgttct agattgtatc tctcgtttca
      361 gatagatctt cgcttcttca gcattgtctg tctccggtac tttcccctct ccaaaaatgc
      421 tcttgacaac gttcaccaca gatacgggca gagatggatc gtccagttcg gttttcagaa
      481 catcgatcaa ctgcgattca ctatccattt tcaatgctct cgttaaggta tctctctgta
      541 acgtagattc cctatcagca atgagcttat agcagtattg cagaacaagc ggggcataca
      601 ccggcataga aggaaagaag actcttctca aaggatatgc cttatcttgg aaaagagagt
      661 ctgggaaagc agtgtctttc aacgcagtga acacttcctt ccacagagca acttcgctcc
      721 taatgttagc cgccttcctt tgaatataga gacagttgat cccaccttta ttgacctcag
      781 acggtttcgg aattccaaca cttgaaaatc tgattctgat attacctgct gtagaggatt
      841 ccagaaggct tctccaaggt tctgggtcaa ggtgagttat ggacattgga tactcagata
      901 tctgctccac aatcttgcag gttggcagat ctcccgctgc gatctgcgct atgctcagac
      961 ttgcttctcc ggcggctcgt gtaatcgtat cccacagatc cgcggtgttt gcgtggtcat
     1021 agaaacaaat cgccagcgtt ttctgttcgt gtttttctac catttgatcc ttaccccaca
     1081 ttcgaggaat tcgtgtgtaa gagcagaatt cccagttcct tcaaaaaggg aactccattc
     1141 atcatatccg ggagtacaat ctgatatccc gcgagtcctg aggtacagga aatcatccaa
     1201 tgccaaactg acgcctgtga gattgccgtt tttcttcttt gttgtgtatt tatctcgaag
     1261 cccatagcgt tgatatgggc taccggaaga atctgttgac cgcaacagta tgcccagttc
     1321 gtatttttca gaagtagagt cagtcat
//
LOCUS       DAOQUC010001313         1346 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_139420_length_1346_cov_3.407436, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001313 DAOQUC010000000
VERSION     DAOQUC010001313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1346
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_139420_length_1346_cov_3.407436"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1005)
                     /locus_tag="PKH07_17910"
     CDS             complement(<1..1005)
                     /locus_tag="PKH07_17910"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNT36870.1"
                     /translation="MQTRSQSFLILFLVFGMCLNIGCGRFSEFVPRIVPDFMVRATAT
                     PTPEIADLIVEPTPEVAPVTEPAPGLMNETELFKAYAKASTLEFEGQLEAALAEYQSV
                     ARRAPESAFVQTRIGVLLQNLGRLEEAHEALVKALELAPEDHAANLAMGMVLYRSGKP
                     ELAEPHFEVATRRGSQRVKAYQEISTLFMAAGDYPKATDAYEKLLSLDPDAAVIYHYN
                     LGQCYLRLNQADKAAEHLEAVAQAQPGHKPIYMTLGQLYERLKQYDKAIDSYRRLLEF
                     DEGNLDARRRIAQIALAQGDHKTAFKEFTLVSEIDPTYLPAYHYLGLIYMQGSMFVQA
                     E"
     gene            complement(1016..>1346)
                     /locus_tag="PKH07_17915"
     CDS             complement(1016..>1346)
                     /locus_tag="PKH07_17915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36871.1"
                     /translation="APLRTARNACPPQRCPESPTPLLLTWEDRWALEIPDSAVVDMRS
                     EQSEVELQITVDGQEQYCLPAGHTLRIRKHPACFKLITSKRRTFYEILREKFNWGDVW
                     QMPSEKC"
BASE COUNT          284 a          387 c          350 g          325 t
ORIGIN      
        1 ttccgcctga acaaacatac tgccttgcat gtaaatcaga cccagatagt gataggcggg
       61 aagataggtt gggtcaattt cggatacgag tgtgaactct ttgaaagccg tcttatggtc
      121 accctgtgca agcgcaattt gagcgattcg tcttcgggca tcgagattgc cctcatcgaa
      181 ctccagaagg cgtcggtaac tgtctatcgc tttgtcgtac tgcttgagtc gctcgtacag
      241 ctgccccagg gtcatgtata tgggtttgtg gccaggctgc gcttgggcaa cagcctcgag
      301 gtgctctgcc gctttgtcag cctggttcaa cctcaggtag cactgaccaa gattgtagtg
      361 atagattacg gctgcatcgg gatccagcga aagcagtttt tcataggcat ccgtcgcctt
      421 gggatagtca cccgccgcca tgaaaagggt gctgatctcc tggtaggcct tgaccctttg
      481 cgaaccgcgt cgcgtggcaa cctcaaaatg cggctccgcc agttccggtt tgcccgatcg
      541 gtacaacacc atgcccatcg ccaaattcgc tgcatgatcc tcaggagcga gttccagcgc
      601 tttgaccagg gcttcatgtg cctcttccaa acgccccagg ttctggagca gcaccccgat
      661 tcgcgtttgc acaaaagccg actcgggtgc ccgtcttgcg acagactgat actcggccaa
      721 ggcggcttcc agctgccctt cgaattcaag agtgcttgcc ttcgcgtatg ctttgaacaa
      781 ttcggtctca ttcatcaagc ctggcgcggg ttccgttacc ggtgcgactt ccggcgttgg
      841 ctcgacgatg agatcggcta tctcaggtgt tggtgtggca gttgctctca ccatgaagtc
      901 aggcacgatc ctcggcacga attccgagaa tcgtccacat ccaatgttga ggcacatacc
      961 gaacaccaga aacagaatca gaaacgactg cgaccttgtc tgcattgtca caacctcaac
     1021 acttctccga tggcatctgc cagacatctc cccagttgaa tttctcgcgc agaatctcat
     1081 agaacgtcct tcttttcgac gtgatcaact tgaaacaagc agggtgcttc cggattcgaa
     1141 gcgtatgtcc cgcaggcagg caatactgct cctgtccatc tacggtgatt tgcagctcca
     1201 cttcggactg ctcggaacgc atgtccacca cagcgctgtc cggaatctcc aacgcccatc
     1261 tgtcctccca cgttaggagt aagggcgttg gtgattccgg acagcgctgt ggtggacatg
     1321 cgttccgagc agtccgaagt ggagct
//
LOCUS       DAOQUC010001314         1346 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_139524_length_1346_cov_1.959721, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001314 DAOQUC010000000
VERSION     DAOQUC010001314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1346
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_139524_length_1346_cov_1.959721"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(151..792)
                     /locus_tag="PKH07_17920"
     CDS             complement(151..792)
                     /locus_tag="PKH07_17920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36872.1"
                     /translation="MSQTSDTPRWICLELKSPLLLAGAVFCASTLLCSIYLYLYGKGI
                     PAYLMYERIVEVESVTEVEDILQCSEYEEAVARWEKRPADGRLLILTRTGGWGIRNIV
                     VEFAKDGRVIRVLHGNHDEMHLGPYPTALKDIAYGIRRLFIGYVVVLTLAFISLLAIV
                     WAFSGKSPQRRSVLLGLAYSVLITEVCLLTVMLTVKICAEMLFLFDHVLPLRF"
     gene            complement(811..>1346)
                     /locus_tag="PKH07_17925"
     CDS             complement(811..>1346)
                     /locus_tag="PKH07_17925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36873.1"
                     /translation="DSDVALYYFGGKRSWFSPERGMYLQHGSAYTLPVPPTFFPPGFR
                     VPEPPQRNPKPPLGIFKPCGPALVIVEPNGCLSCMIAVLSSQYLVTVDPNDKVRHCVA
                     SCELGKQCGGLSDLAFSVGKEIWDSIENLFGFDKEGWSWDDIKADILGIAAKNMQGSC
                     LCNCRRLVDESDLHPDQ"
BASE COUNT          358 a          367 c          313 g          308 t
ORIGIN      
        1 agtagtacag ggcaacgtcg gagtcgtatt cacaacccca gttgtgcttt ctcacgctcg
       61 aatctcccca actgccgttc atctccgctc caaacgaatc ctgctctgtt cttgaaccgt
      121 gcagcagcga gttaagcatc aaaaggtaca tcagaatcgc aaaggaagca cgtggtcgaa
      181 caaaaataac atctctgcac agatcttcac tgtgagcatc accgtgagta ggcaaacttc
      241 cgtaatgagc acactgtagg ctaagcctaa caaaactgaa cgacgttggg ggctctttcc
      301 agagaacgcc caaactatcg ccaatagtga tataaaagca agcgttagga caacgacata
      361 tccaataaac agccttcgta taccgtatgc aatgtcttta agcgccgttg gataaggtcc
      421 cagatgcatc tcatcatggt tcccgtgcaa aacgcggata acacgaccat ccttcgcgaa
      481 ttcaactacg atgttcctta ttccccaccc accggtcctc gtgagaatca gtaggcgtcc
      541 atccgccggt cgcttctccc atcgggcaac ggcttcttcg tactctgagc actgcaggat
      601 atcttcaact tcagtcaccg attctacttc aacaatcctc tcatacataa gatatgcagg
      661 aatacctttg ccatagagat acaaatagat ggaacagagg agggtactcg cacagaagac
      721 tgctcccgcc agcaacagtg ggctctttag ttccaagcat atccaccttg gtgtgtctga
      781 agtctgtgac attactgcat tctcctctga ctactggtct ggatgaagat ctgactcatc
      841 cacaaggcgt ctgcagttgc agagacagga accctgcatg ttcttggccg ctatacccaa
      901 gatgtctgct ttgatatcat cccaagacca accttccttg tcgaaaccga acagattctc
      961 tatcgagtcc cagatctcct ttccgacact gaatgctagg tctgagagac caccacactg
     1021 cttacccaac tcacaagaag ccacacaatg cctgactttg tcatttgggt ctacggtaac
     1081 gaggtattga gaactcaaga cagcaatcat gcagctcagg caaccgttgg gttcaacgat
     1141 gacaagcgcg ggtccgcacg gtttgaagat gcccaacggg ggtttggggt tccgttgtgg
     1201 aggctcaggt actctgaacc caggtgggaa gaacgtcgga ggaacaggca gcgtgtatgc
     1261 actcccatgc tgcaagtaca tgccgcgttc gggggagaac caggagcgtt tgccgccgaa
     1321 gtagtagagg gcaacgtcgg agtcgt
//
LOCUS       DAOQUC010001315         1345 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_139627_length_1345_cov_3.012403, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001315 DAOQUC010000000
VERSION     DAOQUC010001315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1345
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_139627_length_1345_cov_3.012403"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="PKH07_17930"
     CDS             complement(<1..239)
                     /locus_tag="PKH07_17930"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF026171.2"
                     /note="catalyzes the formation of L-threonine from
                     O-phospho-L-homoserine; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HNT36874.1"
                     /translation="MKYISTRGGVEPLSFQQVVLMGLADDGGLLIPESIPDARDRLQS
                     WRKLDYLQLAQEVFSLFATDIPTDVMRSLIERSYRG"
     gene            complement(332..1000)
                     /gene="hypB"
                     /locus_tag="PKH07_17935"
     CDS             complement(332..1000)
                     /gene="hypB"
                     /locus_tag="PKH07_17935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012173727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HNT36875.1"
                     /translation="MKEILVVEKILKANDAVAESNRLEFARRGILVINLMGTPGSGKT
                     ALLERTCVSLRDRTIGVSVIEGDIATTNDAQRLSDKGVQVVQINTDRFGNACHLDASM
                     VCSACCSLNLEDREILFIENVGNLVCPAGFDLGESFRVVVLSVTEGEDKPLKYPVIFR
                     SAHAVILNKTDLLPHLDVSVEQIKMNVLSVQPRCLVFPVSARTGDGFQRWVDWLVGSG
                     KSLG"
     gene            1161..>1345
                     /locus_tag="PKH07_17940"
     CDS             1161..>1345
                     /locus_tag="PKH07_17940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNT36876.1"
                     /translation="MPTYDYECTKCGARFEVFHAMGDQKARQCTKCGKSARRLISAGA
                     GIIVKGASGGLSCSAER"
BASE COUNT          347 a          370 c          323 g          305 t
ORIGIN      
        1 ccccgataac ttctctcaat gagacttctc atcacgtcag ttggaatatc cgtcgcaaag
       61 agagaaaaga cttcttgtgc cagttggaga tagtcgagtt ttcgccacga ctgcaatcgg
      121 tcgcgtgcgt ccggtataga ctcaggaatc agcaatcccc catcgtcagc aagacccatc
      181 aaaacgacct gctgaaaaga gagaggctcc acaccaccac gagttgaaat gtacttcatg
      241 tttcagtcct ttcggctatc acagcactcc aacaatgagt ctcatttata ccatgaaacg
      301 gaaaatggta aagcagtcgc caaccgcttc ttcatccaag agatttccca gagccaacca
      361 gccagtccac ccatcgctgg aatccgtcgc ccgttctggc ggagacagga aagacaagac
      421 atcgaggctg aaccgaaagg acgttcatct taatctgctc caccgaaacg tccagatgcg
      481 gcagcagatc cgtcttgttc aggatcaccg cgtgtgctga tcgaaagatc actgggtact
      541 tcaggggctt gtcttcgcct tcagtcacac tgagaacaac gactcggaac gactcaccca
      601 ggtcaaaacc tgcaggacag accagattcc ccacattttc tataaacaga atttctctat
      661 cctccaagtt cagggaacaa cacgcagagc acaccatgct ggcatccagg tgacaggcgt
      721 tgccgaaccg gtctgtgttg atctgtacca cctgaactcc tttgtccgac aatcgctgtg
      781 catcgttcgt ggtggcgatg tctccctcga taaccgaaac gccgatagtg cggtcacgaa
      841 gagaaacgca ggttctctcc agtagcgccg ttttccctga gcctggtgtc cccatgagat
      901 tgatgacgag aatgccccgc ctcgcaaact caagacgatt ggactccgcc actgcgtcgt
      961 tggccttcag gatcttctcg actacgagga tctctttcat ttgaactctg ccttcttagg
     1021 tctgactgaa cagaatgagc attcctgcca tgttccattg ctgcttgtca agtcagcaag
     1081 ctggaagtca agaatgactt ctggtatcaa tcccacgtac gagacatgat ggtgttcttc
     1141 gacagaaagg ttgagtgaaa atgccaacgt acgattatga atgcacgaaa tgtggtgcac
     1201 gctttgaggt ctttcatgcc atgggcgatc agaaggctcg ccaatgtacc aagtgtggca
     1261 agtcagccag gagactcatc agcgcaggag ctggaatcat cgtgaagggc gcctcaggcg
     1321 gactcagctg ctctgcggaa aggtg
//
LOCUS       DAOQUC010001316         1345 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_139714_length_1345_cov_1.749612, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001316 DAOQUC010000000
VERSION     DAOQUC010001316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1345
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_139714_length_1345_cov_1.749612"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..600)
                     /locus_tag="PKH07_17945"
     CDS             complement(<1..600)
                     /locus_tag="PKH07_17945"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNT36877.1"
                     /translation="MMTDKRDYTDRPVLGSSSLLWWTLLLALALRLVGIWHGLPYAYQ
                     IDEKYVVNHAVGFGTGDLNPHNFHWPGTTIMYLLFVEYGVLFVFGWLIRVFPSPESFA
                     ELFIINPTIFYLVGRVTIAFLGTANVFLGYEIGRRFYSAKVGYVAAFFMAVSSLLTGV
                     DHMVLPDTLLVFLGLMTLLLSHGMMQKGDMKYYLLSGLCI"
     gene            complement(572..>1345)
                     /locus_tag="PKH07_17950"
     CDS             complement(572..>1345)
                     /locus_tag="PKH07_17950"
                     /inference="COORDINATES: protein motif:HMM:NF024584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNT36878.1"
                     /translation="KIRYYSWGEPLLHPDICKMITYANERNIGTELSSNLQQFSKDGP
                     EGLVRSGLELLIASVDGATQATYSTYRVGGKLETVLGNLQALVRTRQAMKKRLPRIEL
                     QFLVMKHNQQDMEEMRVLAKQHGVDRIAFAPLVINTKDPEQVHNWLPDNEKYSRYEYA
                     SLQDRIFRARRRCPWLWRSAVINWDASVSPCCVFEGPKTDFGNLRDQKFADLWNGESY
                     QAARRVFSGRAGGAMEQPTICTRCKGYPEAYDDRQKGLY"
BASE COUNT          338 a          372 c          324 g          311 t
ORIGIN      
        1 aatgcagagt ccggagagga gatagtactt catgtctccc ttttgcatca tgccatgact
       61 taacaacagg gtcatcagac ccaggaagac cagaagtgta tccggtagaa ccatgtgatc
      121 cacaccggtc aacaaacttg aaaccgccat gaagaatgcc gcaacatatc cgactttcgc
      181 agagtagaat ctcctgccaa tctcataacc caagaacacg tttgccgttc ccaggaacgc
      241 gattgtcacc cgtcccacca gatagaaaat ggtcgggttg atgatgaaca gttcagcgaa
      301 ggattccgga gaaggaaaga cgcggatgag ccacccgaaa acgaagagaa ctccgtactc
      361 aacgaacaac aagtacatga ttgtcgttcc aggccagtgg aagttgtggg ggttcagatc
      421 gcctgttcca aatccgacgg catggttgac aacgtatttc tcgtcgatct gatacgcata
      481 ggggagcccg tgccagatcc ccacaagacg caacgcaagt gcaagcagaa gcgtccacca
      541 cagaagggag gatgagccca gaacaggtct gtcagtatag tcccttttgt ctgtcatcat
      601 aggcttcagg ataccctttg cagcgcgtgc aaatcgtcgg ctgctccatt gctccgccag
      661 cccttccaga aaacaccctc cgggcggctt ggtacgattc tccattccat aaatctgcaa
      721 acttttgatc tctcagattc ccaaagtctg tctttggtcc ctcgaataca caacaaggag
      781 agacactggc atcccagttg atgactgcac ttctccagag ccagggacat ctccgccgtg
      841 cccgaaatat cctgtcttgc agactggcat actcatagcg gctgtatttc tcattgtcag
      901 gaagccaatt gtgcacctgc tcggggtcct ttgtgttgat caccaaaggg gcaaacgcga
      961 tccgatcgac accatgctgt ttggccagta cacgcatctc ctccatatcc tgttggttgt
     1021 gtttcatcac cagaaactgc aattcgatcc gtggcaaccg cttcttcatc gcctgccgtg
     1081 ttcgaacaag ggcctgaagg ttgccaagga cagtttccag tttgccaccg actctgtagg
     1141 tagaataagt ggcctgcgtg gctccatcca ccgacgcgat aagaagttcg agtcccgagc
     1201 gaaccaggcc ttccggtcca tccttgctga attgctgaag gttagaactc aactcggtgc
     1261 cgatgtttct ctcattggca taggtgatca tcttgcagat gtctgggtgt agcagcggct
     1321 ctccccaact gtagtagcga atctt
//
LOCUS       DAOQUC010001317         1340 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_140496_length_1340_cov_3.035019, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001317 DAOQUC010000000
VERSION     DAOQUC010001317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1340
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_140496_length_1340_cov_3.035019"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1340)
                     /locus_tag="PKH07_17955"
     CDS             complement(<1..>1340)
                     /locus_tag="PKH07_17955"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HNT36879.1"
                     /translation="LEHLPASGSAPQIKLLLKPRTEPVTDQPKQPKRAGIYLDWLELK
                     YAREMILDSSTLVFQGSTAEETLLPAGSQGDGFYGYEIRLCASHSVVPFVFYLGADGQ
                     ASRIEPYLEDGMLCTPVRSSSYSDGTHTYKICLAAPEAFLPPQKMRLHQPAKLRSPNN
                     KADSIIITHEKFLGSVKRLADEQASKGRSVFVANVEDIYDEFIDGATNPYAIRRFLTF
                     ALRHWQKPAPETVLFFGDASWDYWGRFPNPVPNYVPGYRVDPSYASDQWYVCVSGEDD
                     MEDYYYGRIPVQEVGQAENAIEKIIGYSQGKERGEWEAKTLFFSDNEFEAGLEKAIEG
                     QVPPYLSKEHIRLRDFEFVDNFYMAEVITIQEKAKTSLKGTEYLVKKITEGCLIFEFF
                     GHGSPNVFCHERVFFGGGSKFSDVKKLKNGGRLPLMLLMTCDTGHFDYAEPKWN"
BASE COUNT          325 a          356 c          335 g          324 t
ORIGIN      
        1 ggttccactt tggctcggca tagtcgaaat ggccggtgtc acaggtcatc agaagcatta
       61 acggaagtct tcccccgttc ttgagtttct tgacatcaga aaacttcgaa cccccaccga
      121 aaaacactct ttcgtggcag aagacgttgg gcgaaccatg tccaaagaac tcaaaaataa
      181 gacagccctc agtaatcttc ttaacaaggt attcagtccc tttcaggctc gtttttgcct
      241 tctcctggat ggtgatgact tcagccatat aaaagttgtc tacaaactca aaatcccgca
      301 accgtatatg ttccttggaa agatagggtg gaacttgtcc ctcgatggct ttctcaagac
      361 ccgcctcaaa ctcgttgtcc gagaagaaaa gcgttttggc ttcccattcg cccctctctt
      421 ttccttggga gtaaccgatg attttttcaa tggcgttttc cgcttgcccg acttcctgca
      481 caggaatacg cccgtagtaa taatcttcca tatcatcttc tcccgagaca cagacatacc
      541 actggtcgct cgcgtaggaa ggatcaaccc gatagccagg aacgtaattg ggaacagggt
      601 tgggaaaacg tccccagtag tcccaactgg catctccaaa gaacagcact gtttcaggtg
      661 ccggtttctg ccaatgccga agcgcaaagg tgagaaagcg tcgaatcgca tagggatttg
      721 ttgctccgtc aatgaactca tcgtaaatgt cttcgacgtt ggcgacaaaa actgatctgc
      781 ccttggacgc ctgctcatcg gcaagacgtt tcacgcttcc aagaaacttc tcgtgggtga
      841 tgatgatcga atcagccttg ttgttggggg aacggagctt tgctggttga tgcagccgca
      901 tcttttgtgg tgggagaaaa gcctctggcg cagccagaca aatcttgtac gtgtgcgttc
      961 catccgaata cgaagaggat cgcacggggg tgcagagcat accatcttcc agatacggtt
     1021 caattcggct tgcttgccca tcggcgccca ggtagaacac aaaaggaacg accgaatgtg
     1081 acgcacagag gcgaatctcg tatccataga aaccatcgcc ctgcgatccc gcagggagca
     1141 gagtttcctc agcagtgctt ccctgaaaga caagcgtgct gctgtccagg atcatctcgc
     1201 gggcatactt gagctccagc cagtccagat agatccctgc gcgcttgggc tgtttcggct
     1261 gatcggtgac cggctccgtt cgaggcttca ggagtagttt gatctgcggc gcagaaccgg
     1321 aagcgggcag atgctccagt
//
LOCUS       DAOQUC010001318         1339 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_140711_length_1339_cov_2.538162, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001318 DAOQUC010000000
VERSION     DAOQUC010001318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1339)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1339)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1339
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_140711_length_1339_cov_2.538162"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(118..420)
                     /locus_tag="PKH07_17960"
     CDS             complement(118..420)
                     /locus_tag="PKH07_17960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012805666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HNT36880.1"
                     /translation="MNITSDIKSVTYLKSKAADLLDQINDTHRPVIITQNGEPRAVLQ
                     DAESYENMRNAIGILKILAQGESDIRAGAMRSQSEIFNTIENSLKARQRCAGSIVF"
     gene            complement(620..>1339)
                     /locus_tag="PKH07_17965"
     CDS             complement(620..>1339)
                     /locus_tag="PKH07_17965"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HNT36881.1"
                     /translation="GSFQMGSPDTERGRYSNESPVHTVSIGYDFYMGKWEVTQAQWQA
                     LMGTNPGIGYGVGDDYPVYLVSWDDIRGEDQFIEKLNALGQGTFRLPSEAEWEYACRA
                     GTSTRFYFGDSLGCSDESQDCAAGTLPGNRSDYVWYWYNYDNPTYGCKPVGTKLPNQF
                     GLFDMSGNVREWCEDDWRDHYSDAPVDGSARVYSPRDYYRLYRGGAWGKYFYESRSAS
                     RDYAYPDTKYYTLGFRVAWTP"
BASE COUNT          297 a          407 c          307 g          328 t
ORIGIN      
        1 agatgcttcg cttcgctcag catgacaacc caaagagtgt tttgagacac cctcctcact
       61 atctgaccag tctttccagc aggatatctt cgacgtttcg cctggaatca atgatggtca
      121 gaacacgata cttcctgcgc atcgctgtct tgctttcaag gagttctcga tggtgttgaa
      181 gatctcagac tgcgatctca tagcgccagc ccgaatgtcc gattctccct gcgcgagaat
      241 cttgaggatg cctatggcat tgcgcatgtt ctcatagctc tcagcatctt gcagaaccgc
      301 tcgcggttca ccgttctgag tgataatcac agggcgatgc gtatcgttga tctgatcgag
      361 caaatctgcg gctttggact tcaggtatgt aaccgatttg atgtcgctcg ttatgttcat
      421 atctctctcc ttcagtccaa acaacgtacc ataatcagac cgactttcaa gttcgtaacc
      481 cgcacgcccg tttcctgcgc ggcataaatg ccgtcataca agacccaact gatactcagc
      541 cgttgcgtcc gctccactcg acggacgctg gactactgag gctatcaaca gtgatttcgg
      601 aagtcctggg gctcgttcct tatggagtcc aagcgacacg gaatccgagg gtgtagtatt
      661 tcgtgtccgg gtaggcgtaa tcgcggctgg cagagcgtga ctcgtagaag tatttgcccc
      721 aagccccacc gcgatacaac ctatagtagt cgcgtggact gtacacccgc gcactcccat
      781 ccaccggagc atccgagtag tgatcacgcc aatcgtcctc acaccactcc cgcacgttcc
      841 cgctcatgtc gaacaggcca aactgattcg gcaacttcgt ccccaccggc ttgcagccat
      901 aggttggatt atcgtagtta taccagtacc agacgtaatc cgaccggtta ccgggcagag
      961 tccccgcggc gcaatcctga ctttcgtcgc tgcaacccag tgaatcccca aaatagaagc
     1021 gcgtacttgt tcccgcccgg caggcatact cccactccgc ttcagagggt aaccgaaaag
     1081 tcccttgacc cagggcattc agtttctcga tgaattggtc ttcgcctcgg atgtcatccc
     1141 acgaaacaag gtacactgga taatcgtcgc caacaccata acctattcca ggattcgttc
     1201 ccatcagggc ttgccattgc gcctgtgtca cttcccattt gcccatgtag aagtcgtagc
     1261 caatacttac tgtatggact ggagattcat tcgagtaccg ccccctctcc gtgtccggcg
     1321 atcccatctg gaaactccc
//
LOCUS       DAOQUC010001319         1338 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_140828_length_1338_cov_3.599376, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001319 DAOQUC010000000
VERSION     DAOQUC010001319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1338
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_140828_length_1338_cov_3.599376"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..653
                     /locus_tag="PKH07_17970"
     CDS             <1..653
                     /locus_tag="PKH07_17970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36882.1"
                     /translation="ALSRQFVGKDLYYNAFIGVHAELKWADWWSYDPSSLALWRESIQ
                     PPRPEWLADVAGDSVLLPDVPPVPQETTGTPDLDEASRAAIAFREHSWRAAVGRFTEA
                     IRLGDPNAKISSPLGESYRRFSASMSNLDYWGSSRGADQVVHSYDFFWHAGEESWLAG
                     ASVKAFQGITGLPTEFEFDGPSLLDQHGYTRLGLIAIGQSAAWAGAGLKVANFSYF"
     gene            697..1287
                     /locus_tag="PKH07_17975"
     CDS             697..1287
                     /locus_tag="PKH07_17975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36883.1"
                     /translation="MWNERDAVPSSVTPDKRATVLLFMSKWANYTYREKTEWLHDAQF
                     GVYRMFTQLGIPTRIICEDNLDEDLSGYRAIFRAFSPVELLSSSQRNKLEAVPLPTII
                     DVPSIPSFAANGKVISASGELGKVDISIEGPCPMPLDLSGFGSSYQHALSSTAGSQAA
                     HKPGVVILGYPIGLVYLKGRDRDAQLALLTWALAGM"
BASE COUNT          291 a          350 c          380 g          317 t
ORIGIN      
        1 ccgccctcag ccgtcagttt gtcggcaagg atctgtacta caacgctttt atcggagttc
       61 atgctgaact caaatgggct gactggtgga gctatgatcc gtcgagcctt gcgctctggc
      121 gtgagagcat tcagccaccc agaccggaat ggcttgcgga tgttgccggt gactcagtgc
      181 tgctgccaga cgtgccacca gttcctcaag agacaacagg gacgcccgat ttggatgagg
      241 caagtcgcgc tgccattgca tttcgagagc atagttggag agctgcggtt gggagattta
      301 ctgaagccat tcggctgggc gatccgaatg caaagatctc atcgccgctt ggagaaagtt
      361 atcggcggtt cagcgcttca atgagcaatc tggactactg ggggtcgagt cgtggggccg
      421 accaggtggt gcattcctac gactttttct ggcacgcggg agaggagtcc tggcttgcgg
      481 gagcttccgt gaaagccttt cagggcatca ctgggctgcc aaccgaattt gagtttgacg
      541 gtcccagcct gctcgatcag cacgggtaca cacgtctcgg tctcatcgcc atcgggcagt
      601 ctgccgcatg ggcaggagcg ggactcaagg tcgccaattt ctcctacttc tgagaatctc
      661 ccgtcacagc atgcggttct tctggacttg atcaaagtgt ggaacgaacg cgatgcggtg
      721 ccaagcagtg tgactcctga caagcgggca acagttctgc ttttcatgtc gaaatgggct
      781 aactacacct atcgtgaaaa aaccgaatgg cttcatgatg ctcagtttgg cgtgtaccgg
      841 atgttcacac agctcggcat tccaacccga atcatctgtg aggacaatct cgatgaggat
      901 ctgtcggggt acagagcgat ctttcgggca ttctcaccgg tggaattgct gtcctcatca
      961 caacgcaaca aactcgaagc agttcccttg cccacaatca tagacgttcc cagtatccct
     1021 tccttcgcag cgaacgggaa ggtgatcagt gcttcagggg aattgggaaa agtggatatc
     1081 tcaatcgaag gcccatgtcc aatgccattg gatttgagcg gattcgggag ttcctatcaa
     1141 cacgcactgt cgtccacagc tggttcccag gcagcccaca aacccggagt cgtcattctc
     1201 ggctatccca tcggtttggt ctatctcaaa gggcgtgaca gagatgccca actggctttg
     1261 ctgacttggg cattggcagg aatgtgagat tctgctgcgc caagtgttgg aacaggttcc
     1321 aggattgtgc cgatttga
//
LOCUS       DAOQUC010001320         1337 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_141025_length_1337_cov_2.794852, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001320 DAOQUC010000000
VERSION     DAOQUC010001320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1337
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_141025_length_1337_cov_2.794852"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..629)
                     /locus_tag="PKH07_17980"
     CDS             complement(<1..629)
                     /locus_tag="PKH07_17980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012112573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HNT36884.1"
                     /translation="MGMHSDFPGNPYKIIDPSHRWIPDAGHIIETAKHLRKSGADDTK
                     SVSSPSEDDLDTARRFLIPPLVNRIRQGVKAWRDSYYSGASETTRLLLNWWFNEEHVA
                     HAEQGDASFQYYFAQREAVESALWLYEIEKARDSHALLKYDELDMLSKSDFCGDWTRY
                     VFKLATGAGKTKVMSLLIAWSYFHKLYEEGSELSQNFLVVAPNIIVLERL"
     gene            complement(629..>1337)
                     /locus_tag="PKH07_17985"
     CDS             complement(629..>1337)
                     /locus_tag="PKH07_17985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012112571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HNT36885.1"
                     /translation="VDVLGFDFEMGLVPQAQNEASKKGVHLALKYIPKEVFDREAVRK
                     GHVKFYDAGYLDAALKVLPENKVTITLKGYSIFYQQDADPETLSSLKTGASKVIVENG
                     KVVRVSKDAKGEISKEILTKQWTDWVDYWAVDFNYESRKEIIRVVERNTLQDSHPSPL
                     PEGDKGNTENGIEVEKWTGGYIFENEWQSFRTRKDRTLELTSAPHQYPKKGKYKIAVK
                     VIDLFGNDTTKVLEVKI"
BASE COUNT          300 a          376 c          304 g          357 t
ORIGIN      
        1 agcctttcga gcacgataat gttgggcgcg acaaccagga agttctgtga cagttcgctg
       61 ccttcttcat agagtttgtg gaaatacgac caagcgatga ggagactcat gaccttggtc
      121 ttgcccgcgc ctgtggcaag tttgaacaca tatcgcgtcc agtccccaca gaaatcagat
      181 ttcgaaagca tgtccaactc atcatacttc agcagtgcat gggaatcccg cgctttctcg
      241 atctcgtaaa gccacaatgc agactctacc gcttcccgct gagcaaaata gtactgaaac
      301 gatgcgtcgc cctgctccgc atgagcgacg tgctcctcat tgaaccacca attcaagagc
      361 agccttgttg tctcactcgc ccctgaataa taagagtccc gccacgcctt gactccctgc
      421 cgtattctgt tcaccaacgg gggaatcaaa aaacgtctgg cagtgtccaa gtcatcctcg
      481 gatggactgg aaacgctctt tgtatcgtct gcgccggact tccgaaggtg cttggcagtc
      541 tcgatgatat gaccggcatc cggaatccaa cggtgtgacg gatctataat cttgtatgga
      601 ttccctggga aatcggagtg catacccatc agatcttcac ctccaggact ttggttgtgt
      661 cattgccgaa aagatcaatg accttgacgg caatcttgta tttgcctttc ttcggatact
      721 gatgcggagc ggatgtcagt tccaaagttc tgtccttgcg agtgcgaaaa ctctgccatt
      781 cgttctcgaa gatgtaacca cctgtccact tctcaacttc aattccattt tcagtgttcc
      841 ctttatctcc ctctgggaga gggctggggt gagagtcttg aagagtattc ctctccacca
      901 cccgaatgat ctccttgcga ctctcatagt tgaaatccac cgcccagtag tccacccagt
      961 ccgtccattg tttggtcagg atttccttgc ttatctcgcc tttggcatcc ttgctgacgc
     1021 ggacgacttt tccattctcg acgatgactt tcgacgcgcc tgtcttgagt gatgacaagg
     1081 tctccggatc ggcatcctgt tgatagaaaa tggaataccc tttgagtgtt atggtcactt
     1141 tgttttcggg caataccttc agtgcagcgt ccagataacc ggcgtcgtag aacttcacat
     1201 gacctttcct gaccgcctcc cgatcgaaga cttccttcgg gatgtatttc agcgccagat
     1261 gaacgccctt ctttgacgct tcattctgcg cctgaggaac caagcccatc tcgaagtcga
     1321 agccgagaac atcaacc
//
LOCUS       DAOQUC010001321         1337 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_141074_length_1337_cov_2.222309, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001321 DAOQUC010000000
VERSION     DAOQUC010001321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1337
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_141074_length_1337_cov_2.222309"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(54..129)
                     /locus_tag="PKH07_17990"
     tRNA            complement(54..129)
                     /locus_tag="PKH07_17990"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(94..96),aa:Gln,seq:ctg)
     gene            205..>1337
                     /gene="murJ"
                     /locus_tag="PKH07_17995"
     CDS             205..>1337
                     /gene="murJ"
                     /locus_tag="PKH07_17995"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HNT36886.1"
                     /translation="MSQKQSLLRSTTIVSAGTFISRILGFIRDSVMAYYFHKSLTDPF
                     YAALRIPNALRQLLAEGALSAAFIPTFTQTMEKEGKQEADDLARSLFVILAFVVSVIV
                     VLGIIFSPQLVRLITPGFSQSGEAKMKLTIHLTQIMFPYLLFVSLAALLMGSLHSLGH
                     FTMPALSPVSLNVCIIAFVVCGGFFVPREKLIYLAGFGFMLGGLTQLLIQYPPLFKMG
                     YYRHLPRLLFHPKIGLVGRLLLPATFGQAVTEVNIFVNLYFASRLIEGSVTYLYFAMR
                     LIQFPLGMFGVALATVSFPAFSRLALLEDRKPLVETVKRSVKTNLFLMIPSALGLVVL
                     GPQIIRLLFDRGQFHRDNSLMPTYYAMCCYLVGILAYSSLKLV"
BASE COUNT          246 a          378 c          333 g          380 t
ORIGIN      
        1 tcgttccacc ctacagggct gatttcccaa gggggtggat gaaaagttgg agatggctgg
       61 ggagcaaggg ctcgaacctt ggacctactg atccagagtc agtcgttctt ccaactgaac
      121 tactccccag tggaagctcg caaagaactt acccagacac aaaggccttg tcaagacctt
      181 ttgcaacacg cataatgccg agccatgagt cagaaacaga gtctgcttcg ttccacgacc
      241 atcgtcagtg cgggaacatt catcagtcgg attcttgggt tcattcggga ttcggtcatg
      301 gcgtattatt ttcataagag tctgacggat ccgttctatg ccgcgcttcg tattccgaat
      361 gccctgcgcc aactgctcgc cgaaggtgca ctttccgcgg cgttcatccc aactttcacc
      421 cagaccatgg agaaagaggg caaacaggaa gccgatgacc ttgcccgttc gctttttgtt
      481 attctggctt ttgtcgtttc cgtgattgtt gtgcttggaa taatcttctc gcctcaactg
      541 gttcgcctca ttacgccggg gttttcccag tccggagagg caaagatgaa actgaccatc
      601 catctgaccc agatcatgtt tccctatctt ctgtttgtca gcctggcagc gctgctgatg
      661 ggctctctgc acagtctggg tcatttcacc atgcccgccc tttctccggt ctccctgaat
      721 gtgtgcatca tcgcctttgt cgtctgcgga ggtttttttg ttccgcggga gaaacttatc
      781 taccttgccg gctttggttt catgctgggt gggctgactc aactgctgat ccaatatcct
      841 ccactgttca agatgggtta ctatcggcat cttccgaggc tgctgttcca tcccaagatc
      901 ggtctggtcg gccgtcttct tctgcccgcg acgttcgggc aggcagtcac cgaggtcaac
      961 atcttcgtca acctttactt tgcctcaaga ctcatcgagg gcagcgtgac atatctttac
     1021 ttcgccatgc gtttgatcca gtttcctttg ggcatgtttg gcgttgccct ggctacggtc
     1081 tccttcccgg ctttttccag gcttgccttg ctggaggatc gaaagccgct cgtcgagacg
     1141 gtcaagcgaa gcgtcaagac gaatctcttt ctgatgattc cgtccgctct ggggctcgtc
     1201 gtgctcggcc cgcagatcat ccgcctgctg tttgaccgag ggcagttcca tcgtgacaac
     1261 tctctgatgc ccacctatta cgcgatgtgc tgctatcttg ttggaatcct cgcatattct
     1321 tctctgaagc tggtgtt
//
LOCUS       DAOQUC010001322         1336 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_141199_length_1336_cov_2.734582, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001322 DAOQUC010000000
VERSION     DAOQUC010001322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1336
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_141199_length_1336_cov_2.734582"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1057
                     /locus_tag="PKH07_18000"
     CDS             <1..1057
                     /locus_tag="PKH07_18000"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HNT36887.1"
                     /translation="GWDDVMLTPEQRKKGLRRIEDVLKNDTPILVQMAKEPRGHKGCR
                     LTTKFSIPGRYLVLLPGTNRVMVSKKIVREKEHKRLKEIVTPLRKPGTGFIIRTVSEG
                     ISKSELCQDAKYLLRLWEMAQAEFKKGKKPQLLLQELGVVPRVLRDKWTKTCNEIIVD
                     SQEIRVQVLDTLSLMAPRTPLRKIVKLYEGTGSPFVGMGIEESIAQALSKKVSLQSGG
                     YLLFEEVESLTVVDVNSGRLTRGENIEEIALKTNLEAAEKIAHHMRLRNFGGIIIVDF
                     INLSSKREQEKVLKEFRKHTKADRLVTDISGFSDFGIVQVCRERTRETITKTFTVECP
                     NCQGAGRLPKYRDTGSA"
     gene            1085..>1336
                     /locus_tag="PKH07_18005"
     CDS             1085..>1336
                     /locus_tag="PKH07_18005"
                     /inference="COORDINATES: protein motif:HMM:NF036997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HNT36888.1"
                     /translation="MHAQVAFDIPLDQVFTYLVPESLTSQALEGCRVVAPLGRRERIG
                     FLVSLTEQEPETKVECKEILGVLDRTPPLSGHLLQLTRWV"
BASE COUNT          323 a          336 c          389 g          288 t
ORIGIN      
        1 cggttgggat gatgtgatgt tgactccgga gcaacgcaag aagggccttc gtcgaattga
       61 ggacgttctc aaaaatgaca cgccgatcct ggtgcagatg gccaaggagc ctcgcggtca
      121 caagggctgt cgtttgacga caaagttctc gatcccggga cgttatctcg ttcttctgcc
      181 gggtaccaat cgagtgatgg tttccaagaa gattgttcgc gagaaggaac acaagcgtct
      241 gaaggagatt gtcaccccac tgcgcaaacc gggaacgggc ttcatcatcc gaaccgtatc
      301 ggaaggcatc agcaagtcgg aactctgcca ggatgcaaaa tacctgcttc gactttggga
      361 gatggcacag gcagaattca agaaaggcaa gaagccacag cttctgctcc aggaactggg
      421 cgttgttccc cgagtgcttc gggacaaatg gaccaagacc tgcaacgaga tcatcgtgga
      481 ctcgcaggag attcgagttc aggtgctgga tacgctgtct ttgatggcac cccgaactcc
      541 gctgcgcaag atagtcaaac tctatgaagg aactggttcc ccgtttgtgg gtatggggat
      601 tgaggagagt atcgctcaag cgctcagcaa gaaggtcagt ttgcagtcag gcggttatct
      661 tctctttgaa gaggtcgagt ctctcacagt cgttgacgtg aactcgggac ggttgacccg
      721 tggagaaaac atcgaagaga tcgcactcaa gacaaatctg gaagcggcgg agaagattgc
      781 ccaccacatg cgactgcgca actttggggg gatcatcatt gtggacttca tcaacttgtc
      841 gtccaagagg gaacaggaaa aggtgctcaa agagttccgg aagcacacca aggcggaccg
      901 actggttacg gacatctccg gtttttccga tttcggcatc gtgcaggttt gtcgggaacg
      961 aacacgggag accattacca agacattcac tgtcgagtgt ccgaactgtc agggggcggg
     1021 acgtctcccc aaataccgtg ataccggttc cgcttagggt gggagttcca cacaccgtca
     1081 cagaatgcat gctcaagtcg ctttcgacat accgctggat caggtcttca cctaccttgt
     1141 gcccgagtcg ttgacttctc aggcgcttga agggtgtcgt gtcgtggctc ccttgggacg
     1201 aagggaacgc attggattcc tcgtctccct gaccgagcag gagccggaaa cgaaagtcga
     1261 gtgcaaagag atactcggtg ttctggatcg aaccccccca ttgagtggac acctgctgca
     1321 actgactcgc tgggtg
//
LOCUS       DAOQUC010001323         1335 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_141380_length_1335_cov_2.824219, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001323 DAOQUC010000000
VERSION     DAOQUC010001323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1335
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_141380_length_1335_cov_2.824219"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /locus_tag="PKH07_18010"
     CDS             <1..417
                     /locus_tag="PKH07_18010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36889.1"
                     /translation="CIGLPIIGIVENNENLQLGAFAFLGITLALIVVLLLLSKKHSWI
                     SKLVTWLFANLPGNKLACYSCERYVFALNSTNVNVFTNLGYLCPKCSHVVCSQCAAEA
                     AVKFGANRYMCPACGHELVNDAIIFHKKNMASEEDP"
     gene            complement(745..>1335)
                     /locus_tag="PKH07_18015"
     CDS             complement(745..>1335)
                     /locus_tag="PKH07_18015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36890.1"
                     /translation="SLEGGIGSREETERLTGYGANFGMLYRPLRWMSLGLVYKTAMSM
                     DMTSNLGFRPVKLDLTIPDTLGCGVAVFPNDRLRLAADVDTVHWSRFKDEDVNWLQRD
                     DVLRLHCGAEYLLAMNAEKQRAWFVRAGYMWEESNSIYANSKATAYERLKAPKEDPIN
                     HVTFGFGMALRRFQADIGVDLSRDQTDLIFSTVLYF"
BASE COUNT          316 a          345 c          351 g          323 t
ORIGIN      
        1 tgtatcggtc ttccaattat cgggattgtt gagaataatg agaatctcca gcttggcgca
       61 tttgcgtttt tgggcatcac tctggcgctg attgtcgtgc tgttgttact gtccaagaag
      121 catagctgga tcagcaagtt ggtgacgtgg ctatttgcca atcttcctgg aaacaaacta
      181 gcctgctatt cttgcgaacg ttatgtcttc gcgctaaatt caacgaatgt aaatgtattc
      241 acaaatcttg gctatctctg cccaaagtgc agtcatgttg tttgtagcca gtgtgccgca
      301 gaagccgcag tcaagttcgg ggcgaataga tacatgtgtc cggcatgtgg ccatgagttg
      361 gtcaacgatg caatcatctt ccacaagaaa aacatggcgt ctgaggaaga tccgtaggat
      421 gggtcgtgac ccatcagttg tccgaaatcg gcaggttcgt agtccagttg gttggaacaa
      481 gcgcggatgc tttttgacca ccttaaggaa aaacctgatg accgagcgcg ttccaacatc
      541 ttctttgaat cacgaatggt ggaactcgca cctcttgccc tcaccctagc cctctcccaa
      601 gggcgaggga acatgaaact ggcatgggca tcgtcggcgt tgctaccgac tcaccggtat
      661 agatgagaac ggataagtgg ggacgatgcg tcaagcgaac tgggctttgc gatagagcgg
      721 actcacctgc aggcgctgcc ctgctcagaa atacagtact gttgagaaga tgagatctgt
      781 ctgatctctg gaaaggtcaa cgccgatgtc tgcctgaaag cgtcggagcg ccatgccaaa
      841 tccgaacgta acatggttga tgggatcttc cttgggtgcc ttcaagcgtt cgtaggcagt
      901 cgctttgctg ttggcataga tcgagttgga ctcctcccac atataacccg cacgaacaaa
      961 ccacgcacgc tgcttttccg cgttcatggc aagcagatac tcggcgccgc agtgcagcct
     1021 taacacgtca tctcgttgaa gccagttgac atcttcgtcc ttgaatcgcg accaatgaac
     1081 cgtgtccaca tctgccgcca ggcgaagtcg gtcattggga aacacggcaa caccacaacc
     1141 cagggtatcg ggaatcgtca gatcgagttt caccggccgg aatccaaggt tgctcgtcat
     1201 gtccatggac attgccgttt tgtagaccag accaagagac atccatcgca atggacgata
     1261 cagcattccg aagttggctc catagccggt caggcgttcg gtttcctcgc gggagccgat
     1321 gcccccctca aggct
//
LOCUS       DAOQUC010001324         1335 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_141389_length_1335_cov_2.725000, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001324 DAOQUC010000000
VERSION     DAOQUC010001324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1335
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_141389_length_1335_cov_2.725000"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..944
                     /locus_tag="PKH07_18020"
     CDS             <1..944
                     /locus_tag="PKH07_18020"
                     /inference="COORDINATES: protein motif:HMM:NF027452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HNT36891.1"
                     /translation="AKWCQCAQCIRLGDPVERHALMVSQASGAIRRLKRSVKLECIAY
                     SSYVAPPAKRPVRKDILVDFCPINQCFEHQIDDATSERNADYVNHLREWRKRFAGDLS
                     VYSYYRKYAWNSLPILLPHYMQHDLKFYAAEGINGISSYAEPGDWATYELNHYILGAL
                     AWNPASDMDSLVEEFAICRFGEFADLGCEAYSLLESRARRFSSIPFTAAKSAEEYDGA
                     IAEFQGLLQRVKWAEEITDSSAVLRVLGRLRFGLEYLLLDIMLTKARVMGEGQTSRLA
                     ILSEMDDLLTKASDEGVFLTERLRSTRLASRYGLGDE"
     gene            937..>1335
                     /locus_tag="PKH07_18025"
     CDS             937..>1335
                     /locus_tag="PKH07_18025"
                     /inference="COORDINATES: protein motif:HMM:NF012691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36892.1"
                     /translation="MSRRKAMQGSGRVILAEGGACSELRSEAFGLIIFGASGDLTHRK
                     LIPALFSLYRRRLLHDSFFVLGCARSLMSDEVFRTKVRESLTSLPDGDFSCLDGFLAR
                     LHYVAGDYTVADLYPRLRVAVEGLEQRYGTE"
BASE COUNT          278 a          300 c          394 g          363 t
ORIGIN      
        1 gtgccaagtg gtgtcagtgc gctcagtgca tacggcttgg tgatccggtc gagcgtcatg
       61 cgcttatggt ttcccaggca agtggagcca ttcgacggtt gaaacgttca gttaaattgg
      121 aatgcatcgc ctattccagc tatgttgccc cacctgctaa gcgcccagtt cggaaggaca
      181 ttcttgttga cttctgtcca atcaaccagt gctttgagca tcagattgat gatgcaactt
      241 cggaaagaaa tgccgattac gtgaatcatc taagagagtg gcgcaagcgt ttcgcaggtg
      301 atctgagcgt ctattcgtat taccgaaagt atgcctggaa ctcgcttccg atcctgttac
      361 cacactatat gcagcacgat ctcaagttct acgctgctga aggaatcaat ggcatctcgt
      421 cctatgcgga gccaggagac tgggcgacat acgaactcaa tcactatatc ttgggcgcat
      481 tggcctggaa tcctgcttcc gatatggatt ctttggtaga agagtttgcg atctgccgct
      541 ttggagagtt tgctgacctt ggttgtgaag cgtactctct tcttgagtcg cgtgcgcggc
      601 gcttcagcag cattcctttt actgctgcca agagtgcaga ggaatacgac ggggcgatcg
      661 ccgagtttca gggtctcctg caaagggtga aatgggctga agagattacc gattcctcag
      721 cagtgctgcg tgtgctcggt agattgcgtt tcggtttgga gtacctcttg ttggacatta
      781 tgctgacaaa ggcgagggtg atgggtgagg gtcaaacctc tcggttggca atcttgtccg
      841 agatggacga tttgttgacc aaggcatccg atgaaggtgt cttcttgaca gagcgtttgc
      901 ggtctacaag actggcctct cgttacggtc tgggagatga gtaggaggaa ggcaatgcag
      961 ggctcaggaa gagtgattct tgcggaaggg ggagcgtgtt cggaattgcg ttccgaggct
     1021 ttcgggttga ttatctttgg ggcttctggg gacctaactc atcgaaagct cattccagca
     1081 ctgttcagtc tgtaccggcg caggcttctg catgatagct tcttcgttct gggctgtgcg
     1141 cgctctttga tgagcgatga ggtatttcga accaaggtga gggaatcact cacaagtctt
     1201 cctgatgggg acttcagctg tctggatgga tttcttgccc gtctccacta tgttgctgga
     1261 gactatacgg ttgcagatct gtatccccgg ctgagagtgg cggtcgaagg tcttgagcag
     1321 cggtatggta ccgag
//
LOCUS       DAOQUC010001325         1333 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_141717_length_1333_cov_2.785603, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001325 DAOQUC010000000
VERSION     DAOQUC010001325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1333
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_141717_length_1333_cov_2.785603"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(600..>1333)
                     /locus_tag="PKH07_18030"
     CDS             complement(600..>1333)
                     /locus_tag="PKH07_18030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36893.1"
                     /translation="LYVSLWTRSGLLKQNPKIFLSTSLTPKTYQTSISAIIPDDGPVT
                     LRVELIDRFWNTLASNSRVFAVATPTPTFTVTNTPSPTLTITNTPTNTPTITLTPTPS
                     EWFELDIKATTPESVFPMIVGATQEIAVTLDYRIVTAGSGARLHLSLWTTEGLLAQNP
                     AVFLSPVGNITEYRTSITAAIPDVSSLTMRAQLLDDVWTALAEDSLVFPVATVTPTGT
                     PTFTFTPLPTPTYPNSGVSVWDTYE"
BASE COUNT          334 a          313 c          349 g          337 t
ORIGIN      
        1 agaggagatt cggaagagca atggctgcga gaattccgat gatcgcaaca acaatcagga
       61 gttcaatcag agtgaagccc ttccagggct ccgtcggtcc gtctggtgag gaaagaagtt
      121 cggtgcctcg gtgcatattc atcgttctca gatactacgt acgaaacaca cagaggtcaa
      181 gagtctttct atcattgttc ggtctgttgg agacttcgga agcagggaca aacaacggcc
      241 gcaaaaaaaa tgaggccgaa gccatcttca ctcgcaaagg gggggaaacg tggggttcat
      301 gggggaaaga agatggcctc gaccccaaaa ttgtagttct tatctaatcg gtacttctta
      361 cctcttaatc tcttttgtct cggttatctt ggctcgttcc cagtaaccac ctgtaaaacg
      421 atgtaactct ttttgagtaa cgcaaccaca gaaaactttt ttttggttgc ccatccttcc
      481 tgtcaactct cttcgggtca gaaacccttt gcgagaacaa cgacgaagta tatatcactc
      541 tcgattgctc atttcaactc tttttttcaa aaactctcat ctttttttgt tcccaacaac
      601 tattcatagg tatcccatac agaaacccct gaattcggat aggttggcgt cgggagtgga
      661 gtgaatgtga acgttggcgt cccagtcggc gttacagtcg cgaccggaaa caccagactg
      721 tcctcagcca aggctgtcca aacgtcatcc agaagttgcg ccctcattgt cagagaactg
      781 acgtcaggga tggctgcagt gatgcttgtc cgatactctg tgatattgcc cactggagag
      841 agaaagacgg ctggattctg agccaacagg ccttcggttg tccagagaga caggtgcagc
      901 cttgccccgg aacctgctgt gacaatgcgg tagtccaagg taaccgctat ttcctgagtc
      961 gcgccgacga tcatcgggaa gacggactcc ggcgttgtag ccttgatatc cagttcaaac
     1021 cactcgctcg gtgttggagt caaagtgatc gtaggggtgt tcgtcggagt gtttgtgatc
     1081 gtaagtgtcg gggaaggtgt attggtgact gtgaatgtcg gtgttggtgt tgccacagca
     1141 aacaccctgc tgttcgaggc cagagtgttc cagaatctat caatcagctc gacacgcaac
     1201 gtaacaggtc cgtcgtcagg aataatggca gagatgcttg tctggtaggt cttcggagtc
     1261 agagacgtcg agaggaatat cttgggattc tgcttcaaga gcccactgcg agtccaaaga
     1321 gagacatata gat
//
LOCUS       DAOQUC010001326         1333 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_141782_length_1333_cov_2.032081, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001326 DAOQUC010000000
VERSION     DAOQUC010001326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1333
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_141782_length_1333_cov_2.032081"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(14..520)
                     /locus_tag="PKH07_18035"
     CDS             complement(14..520)
                     /locus_tag="PKH07_18035"
                     /inference="COORDINATES: protein motif:HMM:NF019450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stalk domain-containing protein"
                     /protein_id="HNT36894.1"
                     /translation="MSRVCRLGGLLLVALFVALGKSAAEQYEFSGPKGPIILQGVAVG
                     SQEYYDVFSLAEQAGMNVKWEPLLERLEITSPKGDQVVMIVGMGIYKFNNQAERLQPP
                     IRQEGQLLVNRAALERVGTALGIRVQWQVGHSPEATPTREPKLTLDEILQLLSSSATP
                     ASPLPESS"
     gene            871..>1333
                     /locus_tag="PKH07_18040"
     CDS             871..>1333
                     /locus_tag="PKH07_18040"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNT36895.1"
                     /translation="MNVRGSVRSALVLSVLLCLLPCSCGESFFAPQIKQAGEPVVFLP
                     LGSAFKGFDPAFAGDETSIAAQGLVFEALYQYNYLAEGYELEPCLAEEMGRFSEDGLT
                     YTVRLKQGVVFQDDPCFESTNGKGRPVTVEDFIYSFRRIADVKTRSTGYWIF"
BASE COUNT          289 a          359 c          346 g          339 t
ORIGIN      
        1 tggacgagat tcttcaactg ctctcaggca aaggactggc cggtgtagct gaagaggaga
       61 gcagttgaag aatctcgtcc aaggtcagtt tcggttcccg ggttggtgtg gcttcagggg
      121 aatggccaac ctgccattgt acccgaatgc cgagagccgt gccgactcgt tccaaggccg
      181 cacggttgac caacaattgt ccctcctggc ggataggagg ctgaagtcgc tcggcttggt
      241 tgttgaactt gtagattccc atgccgacaa tcatcacgac ctgatcccct ttgggagagg
      301 tgatctcaag acgttcgagc agaggctccc acttcacgtt catccccgcc tgttccgcca
      361 gcgagaacac gtcataatac tcctgagagc cgacggcgac gccttgcaga atgataggcc
      421 ctttgggacc tgagaactca tactgctccg cggcgctctt gcccagagcg acgaacaaag
      481 cgacaagaag caatccaccg agccgacaga ctcgactcac gctcctcccg tttgtgccag
      541 acaagtaagt catcctgaaa taccagaccc tatttctgac tgcactcaat atctgcatct
      601 cgttgtaacg cacgatgtcc gcaaaagaaa cttggattct ttcaccgatg cgagagcatc
      661 ttagccaacg cagtcgctcc atgcaaggca gatcatcagg acagagattg gagacaattt
      721 gcacgtcctt ttctcgcaga acacattctg tcatccaaat tctgatatgg tcgtccttca
      781 caactcctgg cttgggactc tctcattgta tatctgcgtg tcgcgtctca gagattgaca
      841 ttttgagagg cttcattaca ctttccagct atgaacgtac gcggttcggt tcgatcggct
      901 cttgtacttt cggtgctcct gtgtctgctg ccttgttcct gcggcgagtc gttctttgct
      961 ccgcagatca agcaggcggg agagccggtg gtctttctgc ctcttgggtc ggcgttcaag
     1021 ggatttgatc cagcatttgc cggagatgaa acctctatcg ctgcccaagg tttggtcttt
     1081 gaagcccttt accagtacaa ctatctcgcc gaaggatacg agttggaacc ctgtcttgct
     1141 gaagagatgg ggcgtttctc tgaggatggt cttacgtata ctgtgagact gaagcagggc
     1201 gttgtctttc aggacgatcc gtgtttcgag tccaccaacg gtaaggggag accagttact
     1261 gttgaggact tcatctactc ctttcggcga attgccgatg tcaagacacg ttcgaccggc
     1321 tactggattt tcg
//
LOCUS       DAOQUC010001327         1331 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_142068_length_1331_cov_2.835423, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001327 DAOQUC010000000
VERSION     DAOQUC010001327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1331
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_142068_length_1331_cov_2.835423"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="PKH07_18045"
     CDS             <1..215
                     /locus_tag="PKH07_18045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36896.1"
                     /translation="RCGTRFLIGILLIATVVLNMHQFLTVGWTQLTWYSAAGIIALWL
                     SCLVSSRLRKCGVDSKSDQTDSGERE"
     gene            256..1137
                     /locus_tag="PKH07_18050"
     CDS             256..1137
                     /locus_tag="PKH07_18050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011642153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HNT36897.1"
                     /translation="MKNSHLFTSVLILVGVALTCFCFPVQCQESATKETVMKSAPSQG
                     QLTVEQIVEQSNRVAYYQVKDGRARVQMKITDSQGRIREREFVILRWDQPALDGSDSD
                     EHCGNQKFYVYFRRPADVNKMAFLVWKYVDKDDDRWLYMPALDLVKRIASADKRTSFV
                     GSDFYYEDVSGRNINDDVHELVETTDTYYVLKNTPKSPGSVEFAHYKMWVHKETFMTI
                     KVEYFNVAQEKYREYQALKVDNIQGRNTVTRASMKDLRTGSETTVEYNDVTYDVGVPE
                     DIFSERYLRAAPDKYLR"
BASE COUNT          337 a          308 c          364 g          322 t
ORIGIN      
        1 gccgatgcgg gactcggttt ctcatcggaa ttcttctcat tgcgacggtt gtgctcaata
       61 tgcaccaatt tctcactgtg ggctggactc aattgacctg gtacagcgca gctgggataa
      121 ttgccctgtg gctgagctgt ctggtctctt cacgtctgcg caagtgtggt gtggacagca
      181 aatccgacca gacagacagt ggcgaaaggg agtaagggaa atctggaaga gaacgttgcg
      241 aaaggagcta ctgcaatgaa aaactctcac ttgttcacct cggttcttat actggttggt
      301 gtcgctctga cctgcttctg ttttccggtt cagtgtcagg agtctgcgac gaaggagact
      361 gtcatgaagt ccgctccgag ccagggacag ctgacagtgg aacagattgt cgaacagtcc
      421 aatcgtgtag cctattatca ggtcaaagat ggccgtgcac gggtgcagat gaagattacc
      481 gacagccagg gacgtattcg ggaacgggaa ttcgttatct tgcgttggga tcagcccgca
      541 ctggacggct ctgattccga tgagcattgt ggcaatcaga agttctatgt ctatttccga
      601 aggcctgccg acgtgaacaa gatggctttt cttgtctgga agtatgttga caaagatgac
      661 gaccgctggc tctacatgcc agcgctggat ctcgtcaagc ggattgcctc cgcggacaaa
      721 cgcacgagtt ttgttggctc agacttctat tatgaagacg tatccggacg caatatcaat
      781 gatgatgttc atgagttggt agagacaacg gatacttact atgtgctgaa gaacacccca
      841 aaatcccctg gctctgtgga gtttgcacac tacaagatgt gggttcacaa agagactttc
      901 atgacgatca aagtggaata cttcaatgtg gcgcaagaga aatatcgaga ataccaggca
      961 ctgaaagtgg acaacattca gggtcgcaac acagtgacca gagccagtat gaaggatctg
     1021 cgtacgggca gcgagaccac cgtagaatac aacgatgtca cctatgacgt cggagttcct
     1081 gaggatatct tcagcgagcg ctatctgcgg gcagctccgg acaagtactt gagatagaga
     1141 accacatttg acccaatgaa ggcaataaga gcattcagga aggcagtcag catcatgcca
     1201 cttctcccac tggatagtga gggcaggctg gcgttgtcaa taaaccttgg gatgatcttc
     1261 ctttcggctt tctcactgct tgttgcatcc ggaactgttg gaaatcttcc ggcgaggcgt
     1321 tacaaccgtt g
//
LOCUS       DAOQUC010001328         1330 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_142253_length_1330_cov_2.730980, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001328 DAOQUC010000000
VERSION     DAOQUC010001328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1330
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_142253_length_1330_cov_2.730980"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..570)
                     /locus_tag="PKH07_18055"
     CDS             complement(<1..570)
                     /locus_tag="PKH07_18055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013917142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNT36898.1"
                     /translation="MSDLTSPSRLSVVAPLLEMKGIVKMFPGVQALDHVDFTLQEGEI
                     HAVMGENGAGKSTLIKVLTGVYSNDGGSIVLQGQSIQPRSPVEAQKLGITSVYQEVNL
                     IPYLSVAENISLGREPVRLGCIDWNEVHRRAEYLLKRLDVEIDVTQPLNDYSVAIQQM
                     VAIARALGFKSRILVLDEPTSSLDAKEVGE"
     gene            complement(625..>1330)
                     /locus_tag="PKH07_18060"
     CDS             complement(625..>1330)
                     /locus_tag="PKH07_18060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013376238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNT36899.1"
                     /translation="KVDAIILAPVVETGWEPVLKEARDAGIPVVLVDRGVNVSDDSLY
                     ATLIASDFVEEGRLAAHWLYDKLDGKGSIVQIEGTPGSAPAIDRKRGFEEVVAGCPEL
                     KIIKSQSGDFLRAKGKEVMEAFLKAEGKNINAVYAHNDDMALGAIQAIIEAGMVPGQD
                     IVVVSIDGIKDALQAIVEGTLGASIECNPLLGPKAFDAVESILAGEAVPKKITVADQL
                     FDGLNTSMETVSGREY"
BASE COUNT          322 a          391 c          307 g          310 t
ORIGIN      
        1 ctctccaacc tctttcgcat ccaggcttga cgtcggctca tccaagacga ggattctgga
       61 cttgaatccc aatgcgcggg caatggcaac catttgctga atcgcgacgg aatagtcatt
      121 gagtggctgc gtaacatcga tctcaacgtc aaggcgcttc aacagatatt cagcccgacg
      181 gtgaacctcg ttccagtcaa tgcaccccag tcgaactggc tctcgcccca gggagatgtt
      241 ctcggcaact gatagatacg gaatcaggtt tacttcctgg taaacgctgg tgattccgag
      301 tttctgtgcc tcaacaggag agcgaggctg aatgctctgt ccctgaagaa ctattgatcc
      361 accatcgttg gagtagaccc ctgtcagtac tttgatcaaa gtgctcttcc ctgcgccgtt
      421 ctcccccatc acggcgtgga tctcaccttc ctgaagggtg aaatcaacat gatccaaggc
      481 ctgaacacca ggaaacatct ttacgatgcc cttcatctca agcaggggtg ccaccacgga
      541 aagacgggaa gggcttgtga gatcactcat ggatcggata gaccaactga cagcgattcc
      601 agaagattaa gccaatgccg cggctcagta ttcgcgtccc gaaacggtct ccatacttgt
      661 gttcaagcca tcgaacaact gatctgcaac cgtaatcttc ttgggaacag cttcacccgc
      721 aagaatgctc tcaacggcgt caaatgcctt cgggcccagc agtggattgc attcgatcga
      781 ggctccgagg gttccttcaa caatggcttg cagagcgtct ttgattccgt ctattgaaac
      841 aacgacgata tcttgtccag gaaccatccc cgcttcgatg atcgcttgaa tcgcgccaag
      901 agccatatca tcattgtgtg catagaccgc attgatgttc tttccctcag ccttcagaaa
      961 ggcttccatg acttccttcc ctttggctcg aaggaagtcc cccgactggg acttaatgat
     1021 cttcaattcg ggacaaccgg caacaacttc ttcaaagccg cgcttccggt caatagcggg
     1081 agccgaacca ggcgttccct cgatctgtac gatgcttccc ttcccatcca gtttgtcata
     1141 caaccaatgt gccgcaaggc gaccctcttc aacaaaatca gaagcaatca aggttgcata
     1201 gagcgagtcg tcactgacgt tcacgccacg atctacaagc acgaccggaa tgccagcgtc
     1261 tcgagcttcc ttgagcactg gttcccaacc cgtctccaca acaggagcca ggatgatggc
     1321 gtccactttc
//
LOCUS       DAOQUC010001329         1329 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_142397_length_1329_cov_3.232339, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001329 DAOQUC010000000
VERSION     DAOQUC010001329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1329
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_142397_length_1329_cov_3.232339"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /locus_tag="PKH07_18065"
     CDS             <1..367
                     /locus_tag="PKH07_18065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HNT36900.1"
                     /translation="SRRQGLAERVYARPGVADNIGRVALRRGPVVYCLEGVDNEVPLE
                     RVFLPDKAKLSAQYRDYLLYGTGTIVATALCADDSDWNDDLYRTKPVELKPIKITAIP
                     YCFWNNRGSGEMSVWIKNR"
     gene            364..>1329
                     /locus_tag="PKH07_18070"
     CDS             364..>1329
                     /locus_tag="PKH07_18070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36901.1"
                     /translation="MSQSMSSKDQQILRELAKRKLEIVHDPINVERRDAWYVHQSADE
                     SRPMILAEFAAIVDEKRPFLPELICQDELARHFEYALRSEIWRFEWLRDDYVLEPFFN
                     VSWDVTISDYGVQTKWETSSDAGVLGAKRWDGAIRQGQVGLLQPRTFSVSRETSLQRK
                     HIAEEAFGSILPVRFRGRHWWTMGMSQTAVYLIGLENLMLWMYDDPDGLHQLMRFLCD
                     DHLDFITWYEREGLLTLNNENDYVGSGSMGYTKVLPQPDWREGDPVRCKDCWVLLESQ
                     ETVGVSPELFDEFVLQYQREIGARFGLTYYGCCEPVHSRWEVLKTL"
BASE COUNT          308 a          322 c          386 g          313 t
ORIGIN      
        1 cagtagacga cagggcctcg ccgaacgcgt ctatgccaga cctggtgttg cggacaacat
       61 cggtcgagtc gcacttcgcc gaggccctgt cgtctactgt ctcgaaggcg ttgacaacga
      121 agttccactg gaaagagtct tccttccgga caaagcgaag ctgtccgctc aatatcggga
      181 ttacctgctg tacggtacag gcactattgt tgccacagca ctgtgtgccg atgacagcga
      241 ctggaacgac gatctctatc ggacaaaacc tgttgaattg aagccgatca agattaccgc
      301 gattccctat tgcttctgga acaatcgggg aagcggagag atgagtgtgt ggattaagaa
      361 ccgatgagtc aatcaatgag ctccaaggat cagcagattc tcagagaact ggcgaaacga
      421 aaactggaaa tcgttcatga cccaatcaac gtggaacgca gagacgcttg gtatgtacac
      481 cagtccgctg acgaatcgcg gccaatgatc cttgcggagt ttgcggcgat cgtggacgag
      541 aagcgtccgt ttctgcctga gttgatctgt caagatgaac tggctcggca ttttgagtac
      601 gccctgagaa gtgagatatg gcggtttgag tggcttcggg atgactacgt tctggagccg
      661 ttcttcaacg tgtcttggga tgtcacaatc agtgattacg gtgtgcagac gaagtgggag
      721 accagttcgg atgccggagt gctgggtgcc aagcgatggg acggggccat cagacaaggt
      781 caggttgggc ttcttcagcc tcgaaccttc tccgtttcca gagagacaag cctgcaacgc
      841 aaacacattg ccgaagaagc cttcggcagc attctgccgg ttcgatttcg ggggcgtcac
      901 tggtggacaa tgggaatgtc acagaccgct gtctacctca tcggccttga gaatctgatg
      961 ctgtggatgt atgacgatcc cgacggattg catcagctga tgcgttttct gtgcgacgac
     1021 catcttgatt tcatcacctg gtatgagcgc gagggactac tcacgctcaa caacgagaac
     1081 gactacgtcg gctccggttc catgggctat accaaggtgc ttccacagcc ggactggcga
     1141 gagggtgatc ccgttcggtg caaggactgt tgggtgcttc ttgaatcaca ggagacggtt
     1201 ggtgtcagtc ctgagctctt cgatgagttt gttcttcagt atcaaagaga aattggtgcg
     1261 cgattcggtt tgacttacta tggatgctgc gaacccgttc atagccgttg ggaagtgctc
     1321 aaaactctg
//
LOCUS       DAOQUC010001330         1329 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_142490_length_1329_cov_2.164050, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001330 DAOQUC010000000
VERSION     DAOQUC010001330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1329
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_142490_length_1329_cov_2.164050"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..550)
                     /locus_tag="PKH07_18075"
     CDS             complement(<1..550)
                     /locus_tag="PKH07_18075"
                     /inference="COORDINATES: protein motif:HMM:NF012998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase domain-containing
                     protein"
                     /protein_id="HNT36902.1"
                     /translation="MPDTPEAIRHLREQIDLLDAQIVALLNQRAELAVRIGEEKAKSD
                     LPVFVPSRESEVMRKVTACNIGHFSPESLEIVYREVISGCRCLEERLRVAFLGPEGTF
                     THQAVIKQFGRQVDLLPKQSEMEVFASVERGFADYGVVAAENSLEGVVARVMDLFLDS
                     PLKVCAEIYLPIRHCLLSRSTKE"
     gene            1089..>1329
                     /locus_tag="PKH07_18080"
     CDS             1089..>1329
                     /locus_tag="PKH07_18080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36903.1"
                     /translation="MESKIQALQQVYERVAFSPSGIMYSMLHYDGDAPRPFEPNDVVG
                     KFYFDGRVGDLHLDGPWDYLHGENSIANSGSYLHAQ"
BASE COUNT          297 a          371 c          345 g          316 t
ORIGIN      
        1 cctccttggt cgagcgtgag agaaggcaat gccgaatggg cagataaatc tccgcgcaga
       61 ctttcagtgg tgaatccaag aacagatcca tcacccgtgc aacgacacct tccagtgaat
      121 tctcggctgc aacaactccg tagtccgcaa agcctctttc cacagacgca aagacttcca
      181 tctcgctttg cttgggtagc aagtccacct gcctgccaaa ctgtttgatg accgcctgat
      241 gagtgaatgt cccctctggc cccagaaaag caacgcgaag acgctcttca agacagcgac
      301 atccggagat gacttcccga tagacaattt ccagggattc gggagagaaa tgtccgatat
      361 tgcatgcggt cactttccgc attacctcgc tctcgcggga gggcacgaaa acgggaagat
      421 ccgacttggc cttttcctct ccgatgcgaa cggcgagttc cgccctttga ttcagcaacg
      481 ccacgatctg ggcgtccaac agatcaatct gctctcgaag atgtcgaatc gcttcgggtg
      541 tatcaggcaa cctggaaaac ccctttcctt tagtcagcct gccagcaggc aggtctttgt
      601 ctgaaattca ttgtgaatcc atacacgttt tcacgttgga tttcaagcga agcgaatgtc
      661 cgttcggtat cccggcagtc tcgggtgtcg ctgactggta tgagtcggca gcgtagcgaa
      721 gtctcttcgg ctgtgctcat gatctctccc aacgagatcc gtgacggtca gagtagccgt
      781 tctgtctcca cctcgtggcg tcagtcacat cctgcgacat acatcctgcg actcgttgaa
      841 cgcgctgccg gtctgatcta tagtgcacct gctcttgcga tttgcagacc taacagaaca
      901 tttcggaggt atccgtgaag aaggagaaaa gcccgctcga acaaggagct ccattactct
      961 caagacgatc tgcacttttg gcaggcggag ccggtctggc gggggttctc ctttcaggtt
     1021 gtcgaacttc gaatcctgcc attgccactg gtgcgcctga aagactgtgc atgtctgagg
     1081 aatacagcgt ggaaagcaaa atccaggcgc tccagcaggt ctatgagcgt gttgccttca
     1141 gtccgtcggg catcatgtat tccatgctgc attacgatgg ggatgcgcct cgcccgttcg
     1201 aacccaatga tgttgtgggc aagttttact tcgatgggag agtgggcgac ctgcatctgg
     1261 acggtccttg ggactatctg cacggcgaga actcaattgc caattccggc agctaccttc
     1321 atgcacaat
//
LOCUS       DAOQUC010001331         1328 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_142645_length_1328_cov_2.375491, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001331 DAOQUC010000000
VERSION     DAOQUC010001331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1328)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1328)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1328
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_142645_length_1328_cov_2.375491"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..361
                     /locus_tag="PKH07_18085"
     CDS             <1..361
                     /locus_tag="PKH07_18085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020470131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HNT36904.1"
                     /translation="EPLAPTETDFINTAAEAVRMIHEIGHPRFRLHLDVKAMCSEAQP
                     VDELIRAHAKDTAHFHANDANLRGPGFGDVDYAPIAKALKGVDYQGFVSVEVFNYDPD
                     PETIAIESLAFLKRHLE"
     gene            500..>1328
                     /locus_tag="PKH07_18090"
     CDS             500..>1328
                     /locus_tag="PKH07_18090"
                     /inference="COORDINATES: protein motif:HMM:NF021507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HNT36905.1"
                     /translation="MTGRNPLTSKSVRLHRSIGILLFALLVLVSGFPRAHAQQTLADA
                     DWFEREVGVGVVWRQYLFDSLFNSKQTVSYLDVDLNNPNVSVQFPYLPSSRQRTSAMI
                     PSQFSGSVGGINGTYFDTSGTGGHVTYLRVNGSVIPPGGSLFSPWGYEGALALNASDV
                     ASIQRIPSGGWANNVTHPDIMACGPLLIVDGTIPSAYLTSIGSHCTSRHPRSAVGITA
                     DYHLILLVADGRTEMADGMTCEELAQVMAQLGCPNALNLDGGGSSTLWGAGEQYNGVL
                     "
BASE COUNT          285 a          351 c          362 g          330 t
ORIGIN      
        1 cgagccgctg gcaccaaccg aaacagattt catcaacacg gctgcggaag cggttcggat
       61 gatccacgag ataggccatc cccggtttcg gctccatctg gatgtgaagg ccatgtgttc
      121 agaagcacag ccagtggatg agttgattcg cgctcatgca aaagatacgg ctcactttca
      181 tgcaaatgac gccaatcttc gagggcctgg ttttggcgac gtagactatg cccccatcgc
      241 aaaagcactc aagggagtag attatcaggg ttttgtttcc gtcgaagttt tcaactacga
      301 tcccgatcct gagaccattg ccatcgaaag tcttgcgttc ctgaaacgtc atcttgagtg
      361 acgctccttc agtggttatg ctactctgta cgtacggaaa aaggagcagg ccggcatccg
      421 tgtgctgcgc tactcttctg ttggcttgct tgaatcagaa atcactcccc tttgtcaggg
      481 gtgtcccagg agccatgaga tgacgggaag aaatccgctt acgtcgaaat ctgtccgact
      541 gcacaggtca atcgggattc tcctctttgc actgcttgtg ctggtgtctg gatttcctcg
      601 tgcccacgct caacaaacgt tggccgacgc ggattggttt gaacgagagg ttggtgtggg
      661 ggtagtctgg cgtcagtatc tcttcgacag tcttttcaac tcaaagcaga ctgtcagcta
      721 ccttgacgtt gatctcaaca atccaaatgt ctcggtgcag tttccttatc tgccgagcag
      781 ccgacaacga actagtgcga tgattccgtc acagttctcc ggttcggttg ggggaatcaa
      841 cggaacctat tttgacactt cgggaacggg ggggcatgtc acctatctca gagtcaatgg
      901 atcggtgata ccacctgggg gcagtctctt ctcaccttgg ggttacgagg gagcgcttgc
      961 tctcaatgcc tctgatgttg catccattca gagaatcccc tctggaggct gggcaaacaa
     1021 tgtcacgcat cccgacatca tggcgtgcgg gcctctgctt atcgtagacg gcacgattcc
     1081 ctcagcatat ctgacctcta tcggctctca ctgtaccagt cgtcatccca gaagcgccgt
     1141 cggcatcacg gcggattatc atctcattct tctcgttgct gatggtcgca cggaaatggc
     1201 ggacgggatg acctgcgagg aactcgcgca ggtgatggca caacttggat gccccaatgc
     1261 gttgaatctg gatgggggtg gttccagcac cctgtgggga gcgggagagc agtacaacgg
     1321 tgttctca
//
LOCUS       DAOQUC010001332         1327 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_142801_length_1327_cov_2.814465, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001332 DAOQUC010000000
VERSION     DAOQUC010001332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1327
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_142801_length_1327_cov_2.814465"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..877
                     /locus_tag="PKH07_18095"
     CDS             <1..877
                     /locus_tag="PKH07_18095"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNT36906.1"
                     /translation="GISPPETPMTWEEFIPLAQRLTVRDESGRIKHFGFLFSFHMWNN
                     FVMQWGGRMYSEDGTRCVVDSPEAIRGIQFMHDLVYKYRVTPSPVEEAAMATQGGWGS
                     GNITWFGAGKGAMALGGRWWLCILRDYEGLRLGAFECPHGPNRVFLGYGKSVLINKNS
                     PRREEALNFLVYMSQKPYNDLINHQADALAPVKKFCYTDDYLHDPDFSEEDFNDVWRD
                     IMEYGVPEQISPFVNGNVASRIMTNQLDLVRNNQKPASEAMKTAAELINKEIEKAIER
                     DPSLKEKYEALRRTN"
     gene            879..>1327
                     /locus_tag="PKH07_18100"
     CDS             879..>1327
                     /locus_tag="PKH07_18100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNT36907.1"
                     /translation="MSRRTGRDNEIRTALFFLLPNFVGFVVFTAGPVLFCLAMSFSNW
                     NLQRTIPFQWVWFRNFGELLSDRDFWLYFINTGYLMLGLPVAVFGSLFLAILLNQQLR
                     GIVAYRTLFYLPSITSGVALMILWKVLYNPDFGPINAVLEGFFRHTG"
BASE COUNT          303 a          322 c          359 g          343 t
ORIGIN      
        1 tggaatcagt cctccagaga cacccatgac ttgggaagag ttcattcctc tggcgcaacg
       61 ccttaccgtc cgagatgaaa gtgggcggat taagcacttt ggctttcttt tttcatttca
      121 catgtggaac aactttgtga tgcagtgggg tgggcggatg tattctgaag atggaactcg
      181 ctgcgtggtt gacagccctg aagccattcg gggcattcag ttcatgcacg atctggtcta
      241 caaataccgt gtgactccca gtcctgttga ggaagcagcg atggcaactc agggcggatg
      301 gggatcgggc aacatcacct ggtttggtgc gggcaaaggg gcgatggctc taggcgggcg
      361 ttggtggctc tgcatccttc gtgactatga gggtttgcga ctcggtgcct ttgaatgccc
      421 acatgggcca aacagggttt ttctcggata tggcaagtcg gtgctcatca acaagaacag
      481 tccccggcgc gaagaggctc tgaacttcct ggtctatatg tcccaaaaac cttacaacga
      541 tctgatcaac catcaggcgg atgcccttgc tccggtgaaa aagttttgtt acacggatga
      601 ctacctgcac gatccggact tttccgaaga ggatttcaac gatgtctggc gcgacatcat
      661 ggagtacggt gtgccagaac agatcagtcc attcgtcaac ggcaatgtgg cttctcgaat
      721 catgaccaat cagctcgatc tggtccgaaa caatcagaaa cctgcatcgg aggccatgaa
      781 gactgcagcc gagttgatca acaaagaaat cgagaaagcc atagaacgtg atccgtcact
      841 caaagaaaaa tacgaagcgt tgaggcgcac aaactgagat gagtcgcaga accggaagag
      901 acaacgagat tcggacggca ctgtttttcc tgcttcccaa ctttgtcggc tttgtcgttt
      961 ttacggcagg tccggtgttg ttctgtcttg caatgagttt ctccaactgg aatctgcagc
     1021 gaacgattcc tttccaatgg gtttggttca ggaactttgg agaactgctt tccgataggg
     1081 atttttggct ctacttcatc aataccggat atctgatgct cggattgccg gtggcggtct
     1141 ttgggtcgct tttcctggcg attctcctga atcagcagtt gcgcgggatt gtcgcctatc
     1201 gaacgctctt ctacctgccg agcattacca gcggcgttgc gttgatgatc ctctggaagg
     1261 ttctttacaa tccagacttt ggtcccatca atgcggttct cgagggtttc ttcaggcaca
     1321 caggcat
//
LOCUS       DAOQUC010001333         1325 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_143169_length_1325_cov_2.614961, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001333 DAOQUC010000000
VERSION     DAOQUC010001333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1325
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_143169_length_1325_cov_2.614961"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            571..>1325
                     /locus_tag="PKH07_18105"
     CDS             571..>1325
                     /locus_tag="PKH07_18105"
                     /inference="COORDINATES: protein motif:HMM:NF012239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNT36908.1"
                     /translation="MKEYPLEKKRIVAMTAHNGAGKTSLVEAILFSNGVVERLGSVDS
                     GSAFTDFADEERERHYTIHDAIAEVAPKDHRIQIVDLPGYGDFVKNVRSGLRVADGAI
                     VVVCAVSGVEVETEKCWAYADEFKLPRLVFVNKMDREQADFMKALTQLRKLSETPVVP
                     IQVPIGSQANLQGVVDLVRLQAFYFDEKGKVTRQGEPPADMADMIEEARTMLLDSVAE
                     TDDELCMRYLEGETLKENEIASALGAAAKRGAIV"
BASE COUNT          327 a          334 c          365 g          299 t
ORIGIN      
        1 tccccggaat gttggcgttg agcagaccct ccagaccgtt gatcatcccg tagacttccc
       61 agccgcgctt ctgagcggca tctacggctc ccgcaacttc ccagtcaatg acgggcgttg
      121 gcccgcccga ctctcccagt ataactctct tcttcggcat tcctcaaaac ctctttctgt
      181 catccagaga tgacgagtcc ttatgtgatg aacaaaagca tccgctatcc aggaaacccg
      241 aatataccaa aacagcggag tatttccaga attttatcaa gacagggatc tgagttgcct
      301 gtcgctgcaa tgcgtggggc ttccaagcag gccagcgcct gcaggcgtct gcacagcttc
      361 cgagactgac ccgatactct ggacgggcgt aactgctctg gaccccgaat ctcacaacgt
      421 aatggatttc aatatgttat gatgccacag gtgacaacaa atttctattt acttttcaaa
      481 agagcttcag taatatcaca ttcttgtcaa tatgattgag tacctgaatg aattccgggg
      541 agcccagaat ctggaaagga gctatatgga atgaaggagt accccttgga gaagaaacga
      601 atcgtcgcca tgacggcgca caacggtgcg ggaaagacct cgctggtgga ggcaattctg
      661 ttcagcaatg gtgttgttga acgcttggga agtgtggaca gtggctctgc gttcactgac
      721 tttgctgacg aggagcgaga gcgccactac accatccatg acgccattgc agaggtggct
      781 cccaaagacc atcgcatcca gattgtggat ttgcccggct acggggattt cgtcaaaaac
      841 gttcgaagcg gtttgcgggt agcggatggt gctatcgttg tagtgtgtgc cgtatccggt
      901 gtggaagtgg agacagagaa atgctgggcc tatgccgatg aattcaaact gccccgtctg
      961 gttttcgtca acaagatgga tcgggagcag gcggatttca tgaaggcgtt gactcagctc
     1021 aggaaactgt ccgagacgcc agtcgtgccc attcaggtcc ccattggatc tcaggccaat
     1081 cttcagggcg tggtagactt ggttcgcctt caggcattct atttcgatga aaagggaaaa
     1141 gtgacccgac agggagagcc accggccgac atggcagaca tgatcgaaga agcccgaacg
     1201 atgttgctcg actcggtcgc cgaaaccgac gatgaactgt gtatgagata tctggaaggc
     1261 gaaacactca aggaaaacga gattgccagt gctcttggag ccgcggccaa gcggggggcg
     1321 attgt
//
LOCUS       DAOQUC010001334         1323 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_143535_length_1323_cov_2.605678, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001334 DAOQUC010000000
VERSION     DAOQUC010001334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1323
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_143535_length_1323_cov_2.605678"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="PKH07_18110"
     CDS             complement(<1..302)
                     /locus_tag="PKH07_18110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36909.1"
                     /translation="MSLVSKLVEVHLLNGNRFLGKVKEVDAYGVTVFCIPLTMLEGAV
                     EARNILDDLRIMRHTLFFPHANIEYIDIGGEPLGFDHLFRTWFGSVPLDEFFKSDVV"
     gene            538..>1323
                     /locus_tag="PKH07_18115"
     CDS             538..>1323
                     /locus_tag="PKH07_18115"
                     /inference="COORDINATES: protein motif:HMM:NF014074.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HNT36910.1"
                     /translation="MMKKGLQSCLAAICVMVVLPFATANVSPGDASEEMQPSQEQEFI
                     YTVPINGAVELALRNVIGRALREANRNNAKTIILEIDTLGGRLDAAVEISKMLSGVPQ
                     ETVAFVNTRAISAGALIALSCKRIVMSEGATMGAATPVQLSPTGDTQPTDEKAISYVR
                     AEFRSAAERNGHPPNLAEAMVDKDISIEGVTDEGKLLTLDARQALELRVAESLVENKQ
                     GLLSYLNKESAQEFVLEEYWMEQIARFFANPMISGFLMMLGLLS"
BASE COUNT          338 a          316 c          362 g          307 t
ORIGIN      
        1 accacgtccg acttgaaaaa ctcatccaga ggcacactgc cgaaccaagt acggaaaagg
       61 tgatcgaaac caaggggctc gccgccgata tcaatgtact cgatatttgc atggggaaag
      121 aacagagtgt gacgcatgat tcggaggtca tccaagatgt tgcgagcctc gaccgctccc
      181 tccaacattg tcagggggat gcagaagacg gtcacgccat aggcatccac ttccttcacc
      241 tttccaagaa accggtttcc attgagaaga tgaacttcga ccaacttgct tacgagtgac
      301 ataagaagat gcctccagtg ttcagcacaa ctagtatcaa cttagtgcaa acacgccatt
      361 ttgtccaaca aaacgaagca atggcctcgg agttgacaag tcttgcaggt tcacacagac
      421 taatctgcgt tgggtaacca gaaagggatt tggcgagtat gtccaaacgg attggctcat
      481 tggacggaac ttctgaatat ccctaaggtt tcgaggtatg attttctgga gatagcgatg
      541 atgaaaaagg gcttacagtc ctgtctggcc gcgatatgtg tgatggtggt tttgccattc
      601 gccactgcga acgtttctcc cggggacgcc agcgaggaaa tgcagccatc tcaggagcag
      661 gagtttatct atacggttcc gatcaatggg gcggtggagc ttgctctgcg taatgtgatt
      721 ggtcgagccc ttcgagaagc caatcgcaac aacgcgaaga cgatcattct tgaaatagat
      781 accttgggcg gacggttgga tgcagcagtc gagatctcca agatgctttc gggtgttccg
      841 caggaaacgg tggcatttgt caatacccga gccatttccg ctggtgctct cattgctctg
      901 tcctgtaagc gaatcgtcat gagcgaaggt gctaccatgg gggcggcgac gccggttcag
      961 ctctccccaa cgggtgacac tcaaccgacc gacgaaaagg cgatttccta tgttcgcgcc
     1021 gagtttcgat cagcggcaga gcggaatgga cacccaccaa atctggccga agcaatggtg
     1081 gacaaagaca tcagcattga gggtgtcaca gatgagggaa aactgctgac acttgatgcc
     1141 cgacaggctc ttgaactgcg tgtggctgaa tctttggtgg agaacaaaca aggtcttctc
     1201 agctatctga acaaggagtc cgcgcaggaa tttgtgcttg aggaatactg gatggagcag
     1261 attgcccgtt tcttcgcgaa tcctatgatc tcgggctttc taatgatgtt gggtctgttg
     1321 agt
//
LOCUS       DAOQUC010001335         1321 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_143855_length_1321_cov_3.396524, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001335 DAOQUC010000000
VERSION     DAOQUC010001335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1321
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_143855_length_1321_cov_3.396524"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1321)
                     /locus_tag="PKH07_18120"
     CDS             complement(<1..>1321)
                     /locus_tag="PKH07_18120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HNT36911.1"
                     /translation="SIVIARQVAADIPPATVTVDSTYPGYTSAVLTDGKWYEKGSENP
                     NEWGPDRLGNGGNTWVSTEGSVDHWICLEWSEPVLLDKVIVWWSLAEWYSRAFRLEHL
                     QDGQWTPVRFGEGCEGWLAANDRQTIIPLTSVRTKAIRLLQPARGGGSRAMLAAQEII
                     PLHCGDVSTDLVGARRLSPEAYSRLGSSPLARNIARLSDECTGASFPLVWSAKEKEQP
                     IPALADGDLHAKATDLTRGISAGIRWPVEHSIEGVAATFGETLTQQPSLAVEFHDGRR
                     WTPVRFRLKSHTDFPQRRLEWSFEPLATKAVRIRTPENEDSFALAEIEVYRNLPESKT
                     IWPERFTAPDGLKQEILALPEEPSFEVLSSCALSMRTARALLGLKDTRREVGIVLDGT
                     VDGRSRIEFRIGSEQYRLSEFQDALERVLIDGWRPGVEIRCTFKELEV"
BASE COUNT          300 a          364 c          358 g          299 t
ORIGIN      
        1 ggacttcaag ttctttgaag gtgcatcgga tttccacccc agggcgccag ccgtcaatga
       61 gaactcgttc aagcgcgtcc tggaattcgg aaagacgata ctgttcagat ccgatgcgaa
      121 attcaattct gctgcgtccg tccactgtcc catcaagaac tatcccaact tcgcgcctgg
      181 tatctttgag acccagaagc gcccgtgccg ttcgcatcga gagggcgcag gacgaaagca
      241 cttcgaatga gggctcttcg ggcagagcca ggatttcctg tttgagtccg tctggggcgg
      301 tgaaccgttc aggccagatc gtcttgctct ccgggaggtt tcggtaaacc tcgatttccg
      361 ccagagcaaa cgaatcctcg ttttcaggag tacgaatccg aaccgctttt gttgccagcg
      421 gttcgaaaga ccattcgagt ctcctttgtg gaaaatctgt gtggctcttc aggcgaaatc
      481 tgacgggtgt ccagcggcga ccatcatgga actcaactgc caacgatggc tgttgcgtta
      541 gggtttcgcc gaaggtggca gccacgcctt cgatgctatg ctccacaggc caacgaattc
      601 cagcggatat tcctctggtc aaatctgttg ccttggcatg cagatcgccg tcggcaagtg
      661 cgggaatggg ttgctccttc tctttggcag accagacaag ggggaacgat gcaccggtac
      721 actcgtctga caagcgtgca atgttcctgg ccagtggaga agaacccagg cgggagtacg
      781 cctcgggaga aagtctgcga gcaccgacca gatctgtgga tacatcaccg caatgcagtg
      841 gaatgatttc ctgagcggcc aacatggctc ttgaaccgcc accccttgcg ggttgaagaa
      901 gacgaattgc tttggtgcga acggaagtca gcgggatgat cgtctgacgg tcgttcgccg
      961 ccaaccatcc ctcacatcct tcaccgaatc ttactggcgt ccattggcca tcctgaaggt
     1021 gttccaaacg gaaggcacga gaataccatt cggcaaggct ccaccaaacg atgactttgt
     1081 cgagcaaaac tggctcagac cattccaaac aaatccagtg atccacggaa ccttcagttg
     1141 agacccaggt attgcctcca ttgccgagac ggtcaggacc ccattcattg ggattctcac
     1201 tccccttttc ataccacttg ccgtcagtga gtaccgcaga agtgtagcct ggataggtcg
     1261 agtctactgt aactgtggca ggtggaatat ctgcggcgac ctgtcgggca attacgatgg
     1321 a
//
LOCUS       DAOQUC010001336         1321 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_143927_length_1321_cov_2.411532, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001336 DAOQUC010000000
VERSION     DAOQUC010001336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1321
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_143927_length_1321_cov_2.411532"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /locus_tag="PKH07_18125"
     CDS             complement(<1..226)
                     /locus_tag="PKH07_18125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36912.1"
                     /translation="MATVPLLTIILFRFTGVFVTPSLEFEASLRADGVHLITRALASD
                     MPLMALHLVKDWTLLKQIRHLTHHLAPFGET"
     gene            538..>1321
                     /locus_tag="PKH07_18130"
     CDS             538..>1321
                     /locus_tag="PKH07_18130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36913.1"
                     /translation="MCWVERHSVFDDGSRLIVESSGLVSENSLRLVDRLGTVSALGLP
                     AGYSTAIPLLVARNNRLILIHAKTRSDIVNFSELGDLYVYDMEEERFRLRMERVGTAV
                     AKKVDSVAAVWCLTPKGSLCEIDLLSGETREHFQFPELVQHMRPRCLLTNADGRWLCA
                     VGAYPNVIAWLIDVETGSNFQLRLPEGLGSIDEVEFTSRGIALTVMDDDVRPVRMTLL
                     LGDVPDGEFSPSWSFSGGSRPRFDPRTNTLYFIKQRVKDKWWG"
BASE COUNT          320 a          290 c          382 g          329 t
ORIGIN      
        1 aggtctctcc gaatggtgca agatgatggg taagatgccg gatctgtttc agaagtgtcc
       61 agtctttaac aaggtgcagt gccataagtg gcatatcgct tgccaaagcg cgtgtgataa
      121 gatggacacc atctgcacgc agagatgcct cgaattcaag agatggtgtg acaaacacgc
      181 cagtgaatcg gaaaaggatg atggtaagaa gaggcacagt agccaatggt aattatctca
      241 aatagagcat tttctaggct gaaaggaacc ggggtgatct tggcacttgc tgtcttttcg
      301 tgcaacttcg cctctgcctc atctctccat tttcttctcc cgaggctgca cttgacagac
      361 ggcggaatcc tatcaggaga ccaaggccgc gtaattgaaa tgcttgcccc gaacagggga
      421 gatgatctct ggctgatcag actgaagacg ggcaactcga gtcttgcctt ctccgtaaca
      481 atggtggata agcagatcgg tacaagtaga gatttttacc ttcccaagga gtgggatatg
      541 tgttgggtgg agaggcacag tgtcttcgac gacggttccc gactcatcgt cgagtcaagc
      601 ggactggttt cagagaattc cttacgtctt gtagatagac tcggtacggt gagcgcgtta
      661 ggcttgccag caggttattc gacggcaatc ccgctgctcg tggcgcgcaa caacagattg
      721 attctgattc atgccaaaac tagatcagac atagtgaatt tctctgaact tggcgatttg
      781 tatgtatatg acatggagga agagcgattc cgcctgcgaa tggagcgcgt tggcacagcc
      841 gtggcgaaga aggtagactc ggtggctgcc gtttggtgtc ttacacctaa aggaagtctg
      901 tgtgagattg accttctgtc aggagagact cgcgagcact tccagtttcc ggagttggtg
      961 caacacatgc gtccaagatg ccttttgacc aacgcggacg gtcgctggtt atgtgctgtg
     1021 ggcgcatatc caaatgttat tgcatggctg attgatgttg aaactggcag taattttcag
     1081 ttgcggctgc ccgaaggttt gggaagcatt gacgaggtgg aattcacgtc cagagggatc
     1141 gcgctgacag tcatggatga tgatgtgcga cccgtcagaa tgacgctttt gctgggagac
     1201 gttccagatg gagagttctc tccgtcctgg tctttttcag gtgggagcag accacgtttc
     1261 gacccgagaa cgaacacatt gtacttcatc aaacagcgcg tgaaggacaa atggtggggg
     1321 c
//
LOCUS       DAOQUC010001337         1320 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_144054_length_1320_cov_3.094071, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001337 DAOQUC010000000
VERSION     DAOQUC010001337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1320
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_144054_length_1320_cov_3.094071"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(193..>1320)
                     /gene="dprA"
                     /locus_tag="PKH07_18135"
     CDS             complement(193..>1320)
                     /gene="dprA"
                     /locus_tag="PKH07_18135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HNT36914.1"
                     /translation="LHLVPGIGPRRFRRLLERFGSPERILGASESDLLDVVGVTPQLA
                     QSIRLTSNRKHQELLDRELHEIEEKGVRLYFWGDPDYPQSLAQISDPPCVLYVRGQIL
                     PRDEFSIAIVGSRRISNYGRTMSRTFASELARSGLTIISGFARGADSQAHWGALESGG
                     RTIAVFGNGLNVCYPRENKELLERIVESGALVSELPMNTGPLRENFPLRNRLISALSL
                     GTLVVEAPKKSGALITARYVAEHGKHLFALPGRVGESGSVGPHSLIREGAVLVQSPQD
                     IMRELESLLNRMQEEKGEKTITPVPESVDRPVDAAIPSDLSESERRVLSCISDEAMPM
                     DEVTRKAGISAHEAAGCLLALEMRGLVARLPGMRYILSRRC"
BASE COUNT          298 a          379 c          347 g          296 t
ORIGIN      
        1 gagaagaggt caaacggttg cgaatgcggg aagcgatggt cagagccatg ttcacttcct
       61 cgccgttgaa tccctgctcg ataagcgtgg cggatatctc gtcttccgtc tcttcccagt
      121 tggtggattc gatggctttt cggagcagaa agttgatgat atttatcatt ttcgacatca
      181 aatcggtgac ctctagcacc ttcgactaag aatatagcgc attcctggga gcctggcaac
      241 aagacctcgc atctccaagg ccaaaaggca tccggcggcc tcatgtgctg aaattccagc
      301 ctttcgggtc acttcatcca tgggcatggc ttcgtctgag atacaactca agacgcgccg
      361 ttccgactca ctcagatcgc ttggaattgc cgcgtctaca ggcctgtcaa cagactccgg
      421 cacaggcgtg atcgtctttt cgcccttttc ttcctgcatc cgattcagga gagattccag
      481 ttccctcatg atatcctgag gtgactgaac caacacagcg ccctcccgaa tcagactgtg
      541 gggaccgaca ctgccggatt cccccacccg tcctggaaga gcaaaaaggt gcttcccgtg
      601 ctcagccaca tagcgcgctg tgatcaaagc gccgctcttc ttgggtgctt caacgacaag
      661 agtgccgagg gacaaagcgc tgatcaaccg gttgcgcaga ggaaagtttt cacgcagtgg
      721 ccctgtgttc atcggcagtt ctgacaccag tgctccagac tcaacgatgc gttcgagcag
      781 ttccttattc tcacgcggat agcagacgtt caacccgttg ccaaagacgg caatcgtcct
      841 gccccctgac tccagcgctc cccagtgtgc ctggctgtcc gcacctcggg caaaaccgct
      901 gatgatggtc aagcccgatc gtgccagttc cgaagcgaag gttcgactca tggtgcgtcc
      961 atagttggag attcttcggg agccaactat cgcgatggaa aactcgtctc gtggcagtat
     1021 ttgccctcgg acatagagaa cacagggcgg atcagatatc tgtgccagag actggggata
     1081 atccggatcg ccccagaaat agaggcgaac gcccttttcc tcaatttcat gcagttctct
     1141 atccagaagt tcctgatgct ttctgttgga agtgagtcga atggactgag ccaactgggg
     1201 ggtgactccc acgacgtcca ggagatccga ttcagaggca cccaggatgc gctcgggaga
     1261 tccgaaccgt tctagaagac gtcgaaagcg cctcggaccg atccctggca ccagatgaag
//
LOCUS       DAOQUC010001338         1320 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_144092_length_1320_cov_2.613439, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001338 DAOQUC010000000
VERSION     DAOQUC010001338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1320
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_144092_length_1320_cov_2.613439"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..712
                     /locus_tag="PKH07_18140"
     CDS             <1..712
                     /locus_tag="PKH07_18140"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HNT36915.1"
                     /translation="GQPQLVMELGEKFGRLMNYGDGVYGGQFIGGMYAEAFFETNMER
                     LVEAGLRCIPPKSQYHECISDVLRWYREQPDDWQATWKRIDEKYQRNPQYRRFSCSRE
                     AFNIDAKINGAFVVMGLLYGKGDIERTIEISMRCGQDSDCNPSNATGILFTSLGFSRL
                     PDRYRSALDIQTRFSHTTYNFQELIAVCERLTKRAVSRSGGKIVADAQRRENFLIPMR
                     PPKVPSLEQSWNPVPISH"
     gene            949..>1320
                     /locus_tag="PKH07_18145"
     CDS             949..>1320
                     /locus_tag="PKH07_18145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012414574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HNT36916.1"
                     /translation="MAQTLAEKILSAHVGRAVKPGEIVVTPVDLCFLQDGTGPLAVKQ
                     LQKIGLEKCHNPKKTILFLDHAAPSPRKELSNDHMTLRAFAAKTGAVVCEIGRGVCHQ
                     VVAEHYAKPGEISIGADSHSCT"
BASE COUNT          317 a          364 c          354 g          285 t
ORIGIN      
        1 cgggcaacct cagttggtca tggaactggg cgagaaattc ggccgattga tgaactacgg
       61 cgacggcgtg tatgggggac agttcatcgg cggaatgtac gcggaggcct tctttgagac
      121 gaatatggaa cgcctggtgg aagcgggtct gcggtgcatc cctccgaaaa gccaatacca
      181 tgagtgtatc tcggatgttt tgagatggta tcgagaacag ccggacgatt ggcaggcaac
      241 gtggaagcgg atcgatgaga agtaccagag gaaccctcag tacaggcgct tctcgtgctc
      301 cagggaggcc ttcaatattg acgccaagat caacggcgct tttgtcgtta tgggacttct
      361 ctatggcaag ggagatattg aacgaaccat cgaaatctcc atgcgatgcg gacaggactc
      421 cgactgcaac ccttcaaacg ctacggggat tctcttcacg tctctaggtt tctcgcgact
      481 gcccgaccga taccgttccg cgcttgatat tcagacccgt ttttcgcata cgacgtataa
      541 cttccaagaa ctcatcgccg tctgtgaacg cttgacgaag cgagccgtga gccgttccgg
      601 aggaaagatc gtcgccgacg cgcaaagacg agagaatttc ctcatcccaa tgcgcccgcc
      661 caaggttcca tcgctggagc agagttggaa ccctgttccc ataagccatt gatggaatag
      721 ggactacaca ccattgacgg caacttcccc tccaactctc tgatgtgctc tacgtactcc
      781 agcgagtaac ccggtttccc cgaaaccggg atccagggtt ggtggcaacc caggctgcca
      841 actcacagga caaacaacaa catgattttc ctcttcccct gcaatggctg atccgctatt
      901 ctatcactgt cgcagatgac gccaaatgaa caagtcgagg tttcagatat ggctcaaacc
      961 cttgcagaga aaatcctcag tgcgcatgtc ggtcgggcag tcaagccagg cgagattgtt
     1021 gtcactccag tggatttgtg cttccttcag gacggaacag gtcctctcgc cgtcaaacag
     1081 cttcagaaaa tcggcttgga gaaatgccac aatccgaaga agacgatcct gtttctcgac
     1141 catgccgctc ccagtccgag aaaggaactc tccaacgatc acatgacgct tcgcgccttc
     1201 gcagcgaaga ctggcgcagt ggtgtgtgag attggacgtg gcgtctgcca tcaggtcgtc
     1261 gcggagcact atgccaagcc cggagagatt tccattggcg cggactctca cagttgcacg
//
LOCUS       DAOQUC010001339         1318 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_144404_length_1318_cov_3.403009, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001339 DAOQUC010000000
VERSION     DAOQUC010001339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1318
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_144404_length_1318_cov_3.403009"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1318)
                     /locus_tag="PKH07_18150"
     CDS             complement(<1..>1318)
                     /locus_tag="PKH07_18150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36917.1"
                     /translation="VSYRDSARRRRQAFHLLGASVSERDGRVTKDDDVEYFIDPDRSQ
                     YYFYKFFTNSLGTLFDSKRALKSWDSSAEVKTVTDELGWTAVMRIPFADFERSVPRDG
                     SEWGFNFGTQTGRTNFWVPIHGHETPEKLGTIVFGGENLRPAQLMKVPRIGIGENTLE
                     TQCDRGIRYSIEAMDGNKRCLFRRRGRVAEDGSIRFTLKSDQIDHVNIVLSDVHGNKH
                     LSFWSLFHSPAVTNRLPALLSKAKEVRRRLALFPEDAKIQAERLVRQIQNSQSWIPTD
                     LTQKEWTRFSETVQQFERQIGEAFVYAKTLQRVPGAQFGVGLESPMRKVMIRDLPFEG
                     YVADSYRVKLAGNEHEGVQVVVIPFHRDIENASVRISDVKHAVTGAAFNGKVTASLVG
                     HVMTEEPGLYNPGYTGWYPDPILDFQQSASVAKGEHVSFWIDVATQK"
BASE COUNT          304 a          355 c          334 g          325 t
ORIGIN      
        1 cttctgagtc gccacgtcaa tccagaacga aacatgttca cccttggcga cagaagccga
       61 ctgctggaag tcgaggatcg gatcgggata ccagcctgtg tagccgggat tgtaaagccc
      121 gggttcttca gtcatgacat gtcccaccag agacgcagtg acctttccgt taaacgccgc
      181 tccggtgacg gcatgcttga catcagagat tctcactgac gcattctcaa tgtctctatg
      241 aaatgggatc acgacaactt gaactccctc atgctcattg ccagccaatt tgacgcgata
      301 ggagtccgct acgtagccct cgaagggcag atcgcggatc atgaccttcc gcatggggct
      361 ttcaagtcca actccaaact gcgcaccggg aacccgttgc agagtcttgg cgtagacaaa
      421 ggcttctcca atctgtcttt caaactgttg aacggtttca ctgaagcgtg tccattcctt
      481 ctgtgtcaga tcggtgggaa tccagctctg agaattctga atctgccgaa caagtctctc
      541 cgcttggatc ttggcgtctt ccggaaaaag tgcgagacgc cgacggactt ccttggcttt
      601 gctcaacagt gcgggaagtc ggtttgtgac tgcaggggaa tggaagaggc tccagaagga
      661 aagatgtttg ttgccatgga catccgagag aacgatattg acatggtcaa tctgatcact
      721 tttgagtgtg aaccgaatgc ttccatcctc agcaactcgg cctcttcgcc tgaacaggca
      781 gcgcttgttg ccatccatag cctcgattga gtagcggatg cccctgtcac actgtgtctc
      841 cagagtgttc tcaccgattc caatacgagg tactttcatc agctgagcag gtctcagatt
      901 ctcaccacca aagacgattg tcccgagttt ctcgggcgtt tcgtgaccat ggatgggaac
      961 ccagaaattt gtccgacccg tctgggtgcc gaaattgaag ccccattcac ttccgtctct
     1021 cggtacgctc cgttcaaagt cagcgaaagg gattctcatg actgcagtcc atccgagttc
     1081 atcggtgact gtcttcactt ccgcactgga atcccaactc ttcaaagccc tttttgaatc
     1141 aaaaagcgtt ccgagtgagt tggtgaagaa cttgtagaaa tagtactgac tgcgatcggg
     1201 atcgatgaag tactcgacat catcgtcttt tgtaactctg ccatcgcgct cagaaacact
     1261 tgcgccgagg aggtggaaag cttgtcgcct gcggcgcgcg gagtcgcgat agcttacg
//
LOCUS       DAOQUC010001340         1318 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_144468_length_1318_cov_2.408551, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001340 DAOQUC010000000
VERSION     DAOQUC010001340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1318
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_144468_length_1318_cov_2.408551"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(170..>1318)
                     /locus_tag="PKH07_18155"
     CDS             complement(170..>1318)
                     /locus_tag="PKH07_18155"
                     /inference="COORDINATES: protein motif:HMM:NF024716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HNT36918.1"
                     /translation="GIYKGTITVSADGCKSVPLALEVEVWDFVLPDGTHLKTAFTYSE
                     ALSKRFYKKQWSDELSMKYYDFLLDHRLNIDSLYYSYERDPELMKYGKSRGMNAFNLF
                     YFKGNDTPKELVKEIRIRMERLEKAGVDDIVYFYGFDECDEDQFDKLREICDGVHESF
                     PNLPISTTSYDTSFGLSTGLRNAIDWWVPLTPKYDLEKARALRKEGKQMWWYTCCAPT
                     NPYANWFVEDPAIEARLLMGAMSFKYEVDGFLYFFTNLWELCDTPITSGPYTDWFPGS
                     GGKDRVYANGDGSIFCPGPDGPLTTIRMENIRDGLDDYEYLHMLSELVRKAEQAPDRA
                     SKAEDVRRAKELLAVPDSVVETMIRFTESPEILEDFRQEVAALIQQLK"
BASE COUNT          306 a          337 c          330 g          345 t
ORIGIN      
        1 ggcagcccat cctgttgcca ttcttggtgt ctttttgtct tagtggtgaa tatcctgctc
       61 tgcagtggac gtatggcgga actcatcctg cgcaaagaaa tgattggtat cgaaaagcac
      121 agcacaaatg ctgccgacat ctgcaaacca agaaccagat gctgcatatc tatttcagtt
      181 gttggatgag cgctgcaact tcttgacgga aatcctcaag aatttctggg gattccgtga
      241 accgaatcat cgtttccaca actgaatcag gaactgcaag gagttccttt gctctccgaa
      301 catcctctgc cttgctggct cgatcgggtg cttgttctgc ctttctgaca agctcagaca
      361 acatgtggag atactcatag tcgtccagtc catcgcgaat gttctccatg cgaattgttg
      421 tcagagggcc atcaggtccg gggcagaaga tgcttccatc gccgttggca tagactcggt
      481 ctttgccccc gcttcctgga aaccagtctg tgtaggggcc tgacgtgatg ggagtgtcac
      541 agagctccca gagatttgta aagaagtaca gaaagccatc cacctcgtat ttgaaactca
      601 tggctcccat gagcagccgc gcttcgattg cagggtcttc cacaaaccag ttggcatagg
      661 gattggttgg tgcacagcag gtgtaccacc acatctgttt gccttccttt cgaagagcgc
      721 gtgccttctc caggtcgtac ttgggcgtta gaggtaccca ccagtcaatg gcattgcgaa
      781 gaccggtgct gagaccgaat gaggtgtcgt aagacgtggt tgagatcggc agatttggaa
      841 aggactcatg gacaccgtcg caaatctccc tgagcttgtc gaactggtct tcgtcgcatt
      901 catcgaaccc gtagaaatag acgatatcat ccactccggc tttctcgagc ctttccattc
      961 gaattcgaat ctccttcacc aattcctttg gagtgtcgtt gcccttgaaa tagaagaggt
     1021 tgaacgcatt catccctctg gacttgccgt acttcatcag ttcgggatcg cgttcgtaac
     1081 tatagtaaag ggaatctatg ttaagccggt gatcgagtag gaagtcatag tacttcatcg
     1141 agagttcatc cgaccattgc ttcttgtaga atctcttgct caaagcctca gagtacgtaa
     1201 aggcggtttt caaatgcgtt ccgtccggca ggacaaagtc ccagacctcc acctcaaggg
     1261 caagcgggac ggatttgcag ccgtcagccg aaacggtgat tgtccctttg tagatgcc
//
LOCUS       DAOQUC010001341         1317 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_144627_length_1317_cov_2.716323, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001341 DAOQUC010000000
VERSION     DAOQUC010001341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1317
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_144627_length_1317_cov_2.716323"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1317)
                     /locus_tag="PKH07_18160"
     CDS             complement(<1..>1317)
                     /locus_tag="PKH07_18160"
                     /inference="COORDINATES: protein motif:HMM:NF025224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HNT36919.1"
                     /translation="IQFVYMTGHLDGSGLEGTLHSNNSIVRDYCAANGRTLYDFADIE
                     SYDPDGEYFLDRWADDACNYDGGNWAEEWCASNPGECDFGDSCSDCAHSHCLNCFQKG
                     QAFWWLMARLAGWDGVTGEPTLTPTLEPSETPTPTEHSADTPSETPAPVETETPTLYP
                     TSTNTPVVQVIDPPRADINEDGVVDGKDLLLIQWGWNSLQQGPADIDQNGYVDVRDVF
                     LIGRNWQIGVKFTSTPTCTSTIPPATDTPSSTPSDTTTLTPQESPTNTPTPTVGIVFF
                     PDSHLEQAVRSAINKPKGNILSTDLLELTHLDAGERSITNLEGLQFCINLSSLALYGN
                     HITSITPLLSLHSMEYLYLSDNQIADIGPLGGMSQLRELFLGNNQVVNVTALANKTQL
                     TVLDMPNNSVSDVTPLAGLSALTFLGLNENRVSDLVSLSNLTSLETL"
BASE COUNT          309 a          316 c          351 g          341 t
ORIGIN      
        1 agggtctcaa ggctggtcag gttgctcaat gacaccagat cactgactct gttctcattc
       61 agtccaagaa aagtcagggc tgacagtcct gcgagtggtg tgacatcgga aacactgttg
      121 ttgggcatgt ctaggactgt caactgcgtc ttgtttgcca aggcagtgac gttgacaact
      181 tggttgttgc cgaggaataa ctccctcaac tggctcatcc caccaagcgg cccaatgtcc
      241 gcaatctggt tatctgaaag gtaaagatac tccatcgagt gcaaagacaa gagaggggtg
      301 atacttgtga tatgatttcc gtagagcgcc agagaggaca gattgatgca gaattggagc
      361 ccttccaagt tcgtgatgct ccgctccccg gcgtccaaat gagtcagttc cagaagatca
      421 gtgcttagga tattcccttt cggcttgttg atcgcacttc gcactgcctg ctccagatgt
      481 gaatcgggga aaaagacaat acccactgtt ggcgttggag tgttcgtcgg actctcttgg
      541 ggagttagag tcgttgtatc ggagggtgtc gaagacggtg tatctgttgc cggaggaatt
      601 gttgaggtgc atgttggtgt tgaagtgaac ttcacaccta tctgccaatt cctcccgatt
      661 aagaagacgt cgcgcacgtc cacatatcca ttttgatcaa tgtctgctgg gccttgctgc
      721 agggaattcc aaccccattg gattaggagc aaatcctttc cgtctaccac accatcttcg
      781 ttgatatccg cccgaggggg atcaatgact tgaacaacgg gtgtgttggt tgatgttggg
      841 tagagtgtgg gtgtctccgt ttcaacgggt gcgggtgttt cagagggagt atcggctgaa
      901 tgctcagttg gtgttggagt ctccgagggt tcgagagtag gtgtgagcgt tggttctcct
      961 gtaacgccat cccaaccagc aagccgagcc atcaaccacc agaaggcctg acctttctga
     1021 aagcaattga gacaatgaga atgggcacag tcagaacagg aatcaccaaa atcacattct
     1081 ccgggatttg atgcacacca ttcctcagcc cagtttccac cgtcatagtt gcatgcgtcg
     1141 tctgcccatc tgtcgagaaa atactcccca tcgggatcgt agctttcaat atcggcaaag
     1201 tcgtaaagtg tcctgccgtt tgctgcgcag tagtctcgaa cgatgctatt gttactgtgg
     1261 agcgtacctt ccaatccgct tccatcaaga tggccggtca tatacacaaa ctgaatg
//
LOCUS       DAOQUC010001342         1316 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_144806_length_1316_cov_2.802538, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001342 DAOQUC010000000
VERSION     DAOQUC010001342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1316
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_144806_length_1316_cov_2.802538"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1316)
                     /locus_tag="PKH07_18165"
     CDS             complement(<1..>1316)
                     /locus_tag="PKH07_18165"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HNT36920.1"
                     /translation="ATGMNDGTSWYDAFNDLQDALQLARTDSAITEVWVADGSYSPAS
                     ATGARTAAFDLPDGVGVYGGFGGLDCAEYYNGEWERVQRDASIYEAILTGDLKSDDTA
                     GGDASENCYHVVTARLVGATTTLDGFTIMSGNANGLAASGDNTGGGILIDTASPILAR
                     LIVKNNKATGSGGAIYVRKNSDPNISDCVFLGNTSSLNGGGVYNLGNGTFKNCAFIGN
                     RSNGFGGGIINQDNTAVLLGCLFSGNRAGGAGGGSYNAGSGTKTYINCTFGSNIAVQD
                     GGGIATVMSTVGVSNSILWGNTDRSGTGEIAQLFRQGGNLTVDYSCIRDWTRGGTKNM
                     TDDPQFVLPLGVDGVAGTADDNLRLAPGSPCLDAGNTFALFFENTLEKDLDGAPRIQD
                     DPCAYNTGSSFFFGSPVVDMGAYEHFYPSTFTNKVVYVSALATGNN"
BASE COUNT          288 a          386 c          337 g          305 t
ORIGIN      
        1 tcattgttgc ctgtcgcaag tgcactgacg tacacgacct tattggtgaa cgtggacgga
       61 tagaagtgtt cataggctcc catgtcaaca actggcgatc caaagaagaa gctgctgcca
      121 gtattgtaag cacacgggtc gtcctggatt ctcggtgctc cgtccaaatc tttctcgaga
      181 gtattttcga agaagagggc gaaggtgttg cctgcgtcca ggcagggaga gccaggtgcc
      241 aggcgaagat tatcgtcagc cgttcctgcc acaccatcca caccaagggg cagcacgaac
      301 tgcggatcgt ctgtcatgtt cttggttcca ccacgtgtcc aatccctgat gcaggaatag
      361 tcaacggtca gattgccacc ctgcctgaac agctgcgcga tctcacccgt cccacttctg
      421 tcggtattgc cccacagaat cgagttgctg accccgaccg tgctcatcac agtggcgatg
      481 cctccaccgt cctgaacagc gatatttgag ccaaaggtac agttgatgta cgtcttggtg
      541 ccgctgccgg cattgtacga gccaccaccc gcaccaccag ccctattgcc gctgaagaga
      601 cagcccagta gtacagcggt gttgtcctgg ttaatgatgc ctccaccgaa accattggaa
      661 cggttgccaa taaaggcaca gtttttgaac gtcccgtttc caaggttgta gactccaccg
      721 ccattcagcg acgaggtgtt gcccaggaac acgcagtcgg aaatgttcgg gtcggagttc
      781 tttcgtacat agatggcacc accagaacct gttgccttgt tgtttttcac gataaggcga
      841 gccagaatcg gactcgctgt atcaatgaga atgccgccac cggtgttgtc gccgcttgca
      901 gcaagtccat tggcgtttcc actcatgatg gtgaagccat ccagagtcgt ggtagcaccg
      961 acaagccgtg ctgtgacaac gtggtagcag ttctccgaag cgtctcctcc tgctgtgtcg
     1021 tcgcttttca gatctcccgt caggatcgct tcgtatattg aagcgtctcg ctgaacgcgt
     1081 tcccattctc cattgtagta ctcagcacag tccaagccgc caaagccacc atagactccc
     1141 accccgtcgg gcagatcaaa cgccgccgtt ctcgcccctg tcgcagatgc aggagaatac
     1201 gatccgtccg caacccagac ttctgtgata gcagaatccg ttcttgccag ttggagagca
     1261 tcctgaaggt cgttgaacgc gtcataccag gaggttccat cgttcatccc tgtggc
//
LOCUS       DAOQUC010001343         1316 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_144831_length_1316_cov_2.467090, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001343 DAOQUC010000000
VERSION     DAOQUC010001343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1316
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_144831_length_1316_cov_2.467090"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(65..>1316)
                     /locus_tag="PKH07_18170"
     CDS             complement(65..>1316)
                     /locus_tag="PKH07_18170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36921.1"
                     /translation="KTPDSHGFVAPVGAMPDAVSLDDDNNYFFHLRQGDCDFGQHDCM
                     IESAAADLLFLSEYYLTTRDITALCVSLRKMENIVSLLESRRDESNMFRVGAGANLLA
                     PSYAGSRLDDGSFSWGYHSGVQVTYCAALRRMIEVQKLLNREDKVSEYTAYLNSALEA
                     LPKFLTQEGYLVNYIDQAGIHHGVYGAEQFGYMQTTCNHDAIAFGVFDLETSRKCYRQ
                     LASVTGLWVDGLIRNNYPALDDTYHHWGETETDEYLWRYGVWVNGGIWGTNVARMLLA
                     QFRLDEFADARKCLESFLAEFRQGIAPAIWKGSEFPSPGRPNELDSYAVMGAALRGLW
                     DCRYDATSLTIEPHVPTEIRHLKQKQPLRFGLAQIFVELFNEGTKTVSVTLNGVDLEP
                     KSQIVLPFDSLKKGQNQLVIRMGK"
BASE COUNT          304 a          353 c          366 g          293 t
ORIGIN      
        1 gcgcctcaag aactgtatca aagactgggg ctttgagcct gctggccagt tctgctgtca
       61 gggactactt gcccatcctg atgaccagct gattctgtcc ctttttcaga ctatcaaagg
      121 gcagaacgat ctgggatttc ggctccagat ccactccgtt gagcgtcact gagacagtct
      181 ttgtgccctc attgaagagt tcgacaaaga tttgagcaag gccgaaacga aggggctgtt
      241 tctgcttcaa gtgtcggatt tcggttggaa catgaggctc gatagtcaac gacgtggcat
      301 cgtatcggca gtcccagaga ccccgaagag cagcgcccat gaccgcatag gagtccagtt
      361 cgttgggtct gcccggactc ggaaattcag agcccttcca gatcgcaggg gcaatacctt
      421 ggcgaaattc ggcgaggaaa ctctccagac actttcgcgc atcggcaaac tcatccaggc
      481 ggaattgcgc aagaagcatt ctggcaacgt tggttcccca gataccgcca ttgacccaga
      541 ctccatatcg ccagagatac tcgtccgttt ccgtttcacc ccagtgatga taggtgtcat
      601 cgagtgcggg gtagttgttt cgaatcagac cgtccaccca cagaccggtg acggatgcga
      661 gctgcctgta acacttccgg gatgtctcca ggtcaaacac gccgaaggct atggcatcgt
      721 gattgcaggt tgtctgcata tagccgaact gctctgcgcc gtagacgccg tggtggatgc
      781 ctgcctggtc aatgtaattg acgagatagc cctcttgggt gagaaacttg ggcaaagcct
      841 caagcgccga gttcagatat gctgtgtact ctgacacctt gtcttcgcga ttcaagagct
      901 tctggacttc aatcattcgg cggagcgccg cgcagtaagt aacctgaacg ccggaatgat
      961 agccccaact gaaggaccca tcgtccagtc tggagccggc ataggaagga gcgagcaggt
     1021 tcgctccagc accgacacgg aacatattgg actcgtcgcg tcgggattcc agcaaggaga
     1081 cgatattctc catcttcctg agtgatacgc acagtgcggt aatgtcgcgc gtggtcagat
     1141 agtattccga caagaaaagc agatccgctg ccgcggattc gatcatgcag tcgtgttgtc
     1201 cgaagtcgca atcgccctgt ctaaggtgga agaagtagtt gttgtcatcg tccagcgaaa
     1261 cggcgtcagg catagcgccc acgggagcaa caaaaccgtg ggagtccggt gtcttt
//
LOCUS       DAOQUC010001344         1315 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_144971_length_1315_cov_3.165079, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001344 DAOQUC010000000
VERSION     DAOQUC010001344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1315
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_144971_length_1315_cov_3.165079"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(112..630)
                     /locus_tag="PKH07_18175"
     CDS             complement(112..630)
                     /locus_tag="PKH07_18175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015282687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HNT36922.1"
                     /translation="MVNGIDFRVFRDLSYGLYIVTSKDGDRLNGQLVNTVIQVTSEPP
                     RIAVIINKQNLTHEFIEKSRVFAACVLDESVTMKFLGPFGFRSGRDVDKFAGLSYNEG
                     VTGSPIVVEHVLSFAEAKVIDQMDLGTHTIFVGDVIGTGTLREGQPLTYRFYHENLKG
                     KTPPNAPSYKPQ"
     gene            814..1308
                     /locus_tag="PKH07_18180"
     CDS             814..1308
                     /locus_tag="PKH07_18180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36923.1"
                     /translation="MHRMVCFMTLVSGSTWAASALLAMVLILSVCSMGCATQKREGVV
                     GAQSVGPSGPIDPGQYFRITVVDEDTGRGIPMVRLTSCSNVDFYTDSAGVVAYHEPGL
                     MNLGVFFHVESHGYSIAKDWLGATGRNLKTEPGGSAVLKMKRENIAQRLYRIAQEYTV
                     GTDA"
BASE COUNT          289 a          338 c          370 g          318 t
ORIGIN      
        1 ggctctatcg gagtcgcgat agacctctga acggaggttg acgaaactcc ctccgagcag
       61 gcaagcgcct gctggcgcgt gggctatgcc cctgctcatg caaacgccct gtcattgagg
      121 tttgtaactc ggtgcattcg ggggtgtctt gcctttgaga ttctcatggt agaaccggta
      181 agtgagcggt tgtccttccc gaagcgtgcc agttcctatg acatcgccga cgaagatggt
      241 atgggtgcca agatccatct gatcgatcac tttcgcttcc gcaaacgaaa gaacgtgttc
      301 cacgacgatg ggggatcctg tgacgccttc gttgtaggaa aggccagcga acttgtccac
      361 atctcgtccg gatctgaaac cgaagggacc cagaaacttc atcgtgaccg actcatccaa
      421 aacgcaggcg gcaaaaacac gactcttctc gatgaactca tgggtgagat tctgcttgtt
      481 gatgattacc gcgatgcgtg gcggttccga agtcacctga atcaccgtgt tgaccaactg
      541 accgttgagc cggtctccgt cctttgatgt cacgatatac agtccgtaac tcagatctct
      601 gaaaacacga aaatcaatgc cgtttaccat cgaagccctc ctcgtagagc gttccaaatt
      661 tccgtatgct gtttcgagat gatgaaagag tcaagtggaa aaccatctcg cgccgctcca
      721 cccttctgcc gtgtttgtct ttgtgcggaa ggctgtatat actctcagga aagtcggaat
      781 tcgttccgca gtgttgacgg agggagtaac aaagtgcatc gtatggtttg tttcatgacg
      841 cttgtttcgg gaagtacgtg ggcagcctct gccctgttgg cgatggtgtt gattctctcc
      901 gtgtgctcca tgggttgcgc gacacagaag agagagggcg ttgttggcgc tcagtctgtc
      961 ggcccgagcg gtccgatcga tccaggtcag tactttcgca tcactgtggt ggatgaagac
     1021 accggtcgcg ggattcccat ggttcgactg acgtcatgca gcaatgtgga tttctacaca
     1081 gacagtgcag gcgtggttgc ctatcatgag cccgggctga tgaaccttgg tgtcttcttt
     1141 catgtggaaa gccatggtta ttccattgcg aaagactggc tcggcgctac gggaaggaat
     1201 ctgaagacag agcccggtgg cagcgccgta ctcaaaatga agcgtgagaa tatcgcccag
     1261 cgcctgtacc ggatcgccca agagtacact gtcggcacag atgcctgaac cggtg
//
LOCUS       DAOQUC010001345         1313 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_145429_length_1313_cov_1.899046, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001345 DAOQUC010000000
VERSION     DAOQUC010001345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1313
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_145429_length_1313_cov_1.899046"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..692)
                     /locus_tag="PKH07_18185"
     CDS             complement(<1..692)
                     /locus_tag="PKH07_18185"
                     /inference="COORDINATES: protein motif:HMM:NF022607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2939 domain-containing protein"
                     /protein_id="HNT36924.1"
                     /translation="MSDFKFNCPHCAQSLEAPEDMLGQTIECPACNGSIQLPKPEPQQ
                     KPQMQAPRKRIVVQRHSPPLQSGSTPSPSRRTPPSSKGKVVVPILIVALLFGALYFAS
                     PYWSLFRMRKAVENNDAVYVSDHVDFPQLRESLKATFKTQMAKEVATSDADGFEALGA
                     ALGAMMIGPMVDALVTPEGLIGMMQGKDLGEIDPASPGSSDGSHSTPSEAEMNIAEMG
                     YETLNRFVVKVG"
     gene            complement(799..>1313)
                     /locus_tag="PKH07_18190"
     CDS             complement(799..>1313)
                     /locus_tag="PKH07_18190"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HNT36925.1"
                     /translation="EIVLLYNQYTDTVSGSMPLTLPDTKTSTTYGSASAYGSGGYANA
                     YGSSYTTTYGTKTTYIPYNVRRYDYLATFWTRSKPLSFGAQFRDLTDEQRRQIQSNKG
                     VSVFVVVKGTPAFQNDVLEGDIIRQVNGVDILDVRHCLEVIGKNKGKTVSLTILRNGN
                     EIQKELKLND"
BASE COUNT          268 a          343 c          367 g          335 t
ORIGIN      
        1 tctccgactt tgacaacgaa ccggttcagg gtctcgtacc ccatttccgc tatattcatc
       61 tccgcttcag agggcgttga atgcgagccg tcacttgagc ctggggatgc agggtctatc
      121 tcgcccaaat ccttgccttg catcatcccg atcaggccct cgggagtcac cagcgcatcg
      181 accatcggcc caatcatcat cgcgcccagc gccgccccca gagcctcaaa accatccgca
      241 tcagatgtcg ccacctcctt ggccatttga gtcttgaatg ttgctttgag gctttcgcgg
      301 agttggggaa agtctacgtg gtccgacaca tataccgcgt cattgttctc taccgctttc
      361 cgcatccgga agaggctcca gtacgggctc gcgaaatata gagcgccgaa aagaagcgca
      421 acgatgagaa tcggtaccac aaccttgccc ttcgaggagg gtggggttct tcgagaagga
      481 gaaggtgttg atcccgactg caagggaggc gagtggcgtt gcaccacgat tcttttgcga
      541 ggcgcttgca tctggggttt ttgttgcggc tcgggtttgg gtaactggat agagccattg
      601 cacgccggac actcgatcgt ctggccgagc atgtcttcgg gcgcttcgag cgattgcgca
      661 cagtgtggac agttgaactt gaaatcgctc atcggctgac ctcttccttt tctctctcgc
      721 taacgccacc cctcaccgga cgctggaaga cgcgcaacgc gcggctttca gcggtacgag
      781 tgcaggggtt tgttctgctc agtcattcag tttgagttct ttttggattt cgttcccgtt
      841 gcgaagaatg gtcagagata cagtcttccc tttgttcttg ccgattacct caagacaatg
      901 gcgaacatca agtatatcca ccccattgac ttgacgaatg atgtcccctt cgagaacgtc
      961 attctgaaat gcgggagttc ctttgacgac aacgaacacg gaaactcctt tgttggattg
     1021 tatctgccga cgctgttcgt cggtcaggtc tcgaaactgc gctccgaagg agagcggttt
     1081 tgatcgagtc caaaaggttg cgagatagtc gtaccgacga acattgtatg ggatataggt
     1141 tgtcttggtg ccgtatgtgg ttgtgtagga tgagccgtat gcgttggcgt agccaccaga
     1201 cccataggct gaggcggaac cataggtcgt ggatgtcttt gtgtcgggaa gcgtgagagg
     1261 catgcttccc gatacggtgt ctgtgtattg attgtagagc agtacgattt cag
//
LOCUS       DAOQUC010001346         1312 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_145530_length_1312_cov_2.788385, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001346 DAOQUC010000000
VERSION     DAOQUC010001346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1312
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_145530_length_1312_cov_2.788385"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            128..>1312
                     /locus_tag="PKH07_18195"
     CDS             128..>1312
                     /locus_tag="PKH07_18195"
                     /inference="COORDINATES: protein motif:HMM:NF019556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HNT36926.1"
                     /translation="MMRSPCWIRRCLILLCHLFIIVASVASEEVKSMNEEMNFDTGLV
                     PPAFQPLPLGSIKPEGWLLRQLQIQAQGPSGHLDEFWASVGDSAWFGGTGDAWERGPY
                     WLDGLVPLAYVLDDELLKVKVKRFMDYILSSQQQDGWIGPVDYSKKQDQTFSYDPWPA
                     FVALKALTQYEEATGDERVIPAMQRFFKKLDALLDEKPLFSWGMFRWMDCVLSVHWLY
                     ERKPEPWLLELGSKIQEQGFDWQGHYADFKYKGKTLKDQTKMETHVVNNAMAVKQAGV
                     WFRQSKDMADRKAVDHCLDILDQYHGTAVGTFTGDEHYAGKHPSQGTELCAVVEYMFS
                     LENLISFFGDVAYCDRLEKIAYNALPAQFTPDMWGHQYDQQVNQVLCIRSDDHIWETN
                     GPE"
BASE COUNT          298 a          310 c          372 g          332 t
ORIGIN      
        1 ggttcgaatc cctgagccgc taccagtttt ccttctgaat catccttttt ctgccatgtg
       61 cgtttttctg atctttgatt ttcgtgtgtg atgtatatta cctgcaacct tttgggaagg
      121 aatcttgatg atgagatctc cgtgctggat tcgaaggtgt ctgattctgt tgtgtcattt
      181 gttcatcatt gtcgcctccg tggcgtcaga agaggtaaag agcatgaacg aagagatgaa
      241 tttcgatacc ggtcttgttc ccccggcctt tcagccgttg ccgttgggca gtatcaaacc
      301 tgagggctgg cttctgcgac aactgcagat tcaggcgcag ggtcccagtg gacacctcga
      361 tgagttctgg gcgtctgtgg gtgacagcgc gtggtttggt ggcacgggtg acgcctggga
      421 gcgaggccct tactggttgg acggtctcgt gcccttggct tacgtgctgg acgatgaatt
      481 gctgaaagtg aaagtcaagc ggtttatgga ctacattctg tcatcgcagc agcaagacgg
      541 ttggatcgga cctgtggact acagcaagaa acaggaccag acgttcagct atgatccctg
      601 gcctgctttc gtcgcactca aggcgttgac ccagtatgag gaagccactg gggatgagcg
      661 cgtgattccc gcaatgcagc ggttcttcaa gaagttggat gctttgctgg acgagaaacc
      721 gctcttcagt tggggtatgt tccgttggat ggactgtgta ctctccgttc attggctcta
      781 cgaaagaaag cctgaaccgt ggcttctgga acttggcagt aagattcaag agcaaggctt
      841 tgactggcag gggcactacg cagatttcaa atacaaaggg aaaactttaa aggatcagac
      901 caaaatggag acccatgttg tcaacaacgc catggcagtc aagcaggctg gggtctggtt
      961 cagacagtcc aaagacatgg ctgaccgcaa ggcggttgac cactgtctgg atatcctgga
     1021 tcagtatcat ggaacagccg ttggaacctt cactggtgac gaacactacg ctggcaagca
     1081 cccatcacag ggaacggaac tctgcgcggt ggttgagtat atgttctcat tggagaacct
     1141 gatcagtttc tttggtgatg tcgcctattg cgaccgtttg gaaaagatcg cctacaatgc
     1201 actgccagcg cagttcactc ccgatatgtg ggggcaccaa tacgaccagc aagtcaacca
     1261 ggtgctctgt atccgtagcg acgatcacat ctgggaaacc aatggcccgg ag
//
LOCUS       DAOQUC010001347         1310 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_145882_length_1310_cov_2.819124, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001347 DAOQUC010000000
VERSION     DAOQUC010001347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1310
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_145882_length_1310_cov_2.819124"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1005)
                     /locus_tag="PKH07_18200"
     CDS             complement(<1..1005)
                     /locus_tag="PKH07_18200"
                     /inference="COORDINATES: protein motif:HMM:NF016906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HNT36927.1"
                     /translation="MRPKFVLTALVILSVSSVASADYSTPGAGTSYTLADLAQIAPEV
                     VQQETDFNFRILDKLTIAASDELKIFDSFVTMATRQSVKLGWNSTDDFMPIIEIEGRI
                     QAGNTIFRADVSSEYENAPHALVLRTSQSDQPSGVIEDCTFERFFTAITARHPGQVFI
                     NSCRFNECYSIGVHAFQESHVEISDCVFYNCAAVSAEGTVKIRNCRAEGGGITLSSIG
                     QGSEIADNVLIGPGQFGLLVVGTVTPTVERCQISGFQYGLIVEGSGEAQFESNSIWGN
                     RYGAVLFTDYARIRMRRNQIVHNTAEILDAKGAVSMPAICIDLNATVDLGTTSSPGFN
                     I"
     gene            complement(1118..>1310)
                     /locus_tag="PKH07_18205"
     CDS             complement(1118..>1310)
                     /locus_tag="PKH07_18205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36928.1"
                     /translation="AQGNDRAKPASGWWLNGKVLRRRLLPRRMLDLYQFLVPLFRFIE
                     KVSGPPAGLSLVVVLKRKP"
BASE COUNT          314 a          359 c          328 g          309 t
ORIGIN      
        1 gatgttgaac cctggtgatg aggttgtgcc gagatccacg gtggcattga ggtcaatgca
       61 gatcgccggc atgcttacag cacccttggc gtcaagaatc tccgctgtgt tgtgcacaat
      121 ctgattcctt ctcattcgga tacgggcata gtcagtgaac aacacagctc catatcggtt
      181 gccccaaatg ctgttgctct caaactgcgc ttcacctgaa ccttcgacga tgagaccgta
      241 ttggaagcca gaaatctggc acctctcaac ggtcggtgtc actgtgccaa caacgagcag
      301 gccgaactga ccggggccta taaggacatt gtctgcgatt tcactccctt gtccgatgct
      361 ggatagagtt attcccccac cctctgctcg gcagttccgg atcttgaccg ttccttcagc
      421 agaaactgcc gcacagttgt agaaaacaca gtctgagatt tcgacgtggc tctcctgaaa
      481 cgcgtgaacg ccaatgctgt aacactcatt gaaccgacag gaatttatga agacctgacc
      541 tggatgccgt gccgtgatag cagtgaaaaa ccgttcaaag gtgcagtctt caatcactcc
      601 cgacggctga tcgctttgac tggttcggag caccaacgca tgtggagcat tctcatactc
      661 tgaggacaca tcagctcgaa agatcgtgtt gccagcctgt atgcgacctt ctatctcaat
      721 aatcggcatg aaatcgtctg tgctgttcca acccaatttc accgactgcc ttgtcgccat
      781 tgtgacgaac gagtcaaaga ttttcagttc atcgctggcg gcgatggtca acttgtccag
      841 aatgcggaag ttgaaatctg tctcctgctg gaccacctcg ggtgcaatct gtgccaagtc
      901 cgccagtgta tacgatgttc ccgctccagg cgtcgagtag tccgcagacg cgacagagga
      961 cacactcaaa atgaccaacg ctgtcaagac gaatttgggt ctcatggtct accccctgaa
     1021 attgtatcta taatctactg tcccacagat ccctcttctc tgtcaaagat tttctggggg
     1081 aagtaagagt tggaaagagg cagaaaagac actgtcccta aggttttcgc ttgaggacta
     1141 cgaccagtga gagtcccgcc ggaggaccgc tcaccttttc gatgaaccga aacaaaggga
     1201 cgaggaactg atagagatcg agcatcctgc gcggcaggag tctccgacga agtaccttgc
     1261 cattgagcca ccagccactg gcgggtttgg cgcggtcatt gccctgcgcc
//
LOCUS       DAOQUC010001348         1309 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_146043_length_1309_cov_3.337321, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001348 DAOQUC010000000
VERSION     DAOQUC010001348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1309
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_146043_length_1309_cov_3.337321"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /locus_tag="PKH07_18210"
     CDS             <1..371
                     /locus_tag="PKH07_18210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36929.1"
                     /translation="PTDTFTFTATHTPTGTLQPTETPTMTPTDSPTSAATPTPTESLS
                     PTDTPLTTPVETPTPIPTMKSADFNGDYRVDGMDLLTLLSNLGRLGNLQTDLNDDGRT
                     DSMDVLLFAQQWNRRYSPDQ"
     gene            complement(398..>1309)
                     /locus_tag="PKH07_18215"
     CDS             complement(398..>1309)
                     /locus_tag="PKH07_18215"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF013319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HNT36930.1"
                     /translation="ADPALVSKEARKRGRDQLGTLGAGNHFLEIQVVDEVYDAKAAEA
                     LGLQKGQVTVMIHCGSRGLGHQACTDSINLMRRSRNKFKFDLPDAELVCAPVSSDEGQ
                     EYLKTMNAAANFALANRQCIMHWVRETFEQIFGRGWQKLGMTLVYDVSHNIAKIEQHR
                     VGSVEKELCVHRKGATRCFPAGHPSVVPCYRSLGQPVIVPGNMGDASYVLLGQPEALL
                     KSFGSTCHGAGREMSRNQAIKAGRGRSIEHELGEKGIQVRAHSRETLSEEMPEAYKNI
                     DQVVSVVEGAGLSSKVVRLKPVGVIKG"
BASE COUNT          313 a          399 c          321 g          276 t
ORIGIN      
        1 cacccacaga caccttcact ttcacggcca cccacacgcc aacgggaaca ctgcagccga
       61 ccgaaacgcc aacgatgact ccaaccgatt caccgacgtc tgccgcaaca ccaacgccta
      121 cagaatcctt atcaccgaca gacaccccat tgacaacccc agtggaaaca ccgacaccaa
      181 ttccaaccat gaagtccgcg gatttcaatg gtgactacag ggtggacgga atggatctgc
      241 tgacattgct gtcaaatctc ggcaggcttg gaaacctgca gaccgatctc aacgacgacg
      301 ggagaactga ttcgatggat gttctgctct ttgcgcagca gtggaatcga agatacagcc
      361 ctgatcagtg acgacaaaga ggcgtgcaag gcgtctgcta ccccttgatg acaccgacag
      421 gtttcaagcg gacaaccttg gacgagagac ctgctccctc cacaacggag acaacctggt
      481 ctatgttctt gtatgcttcg ggcatttctt cggagagcgt ttcgcggctg tgggcacgaa
      541 cttgaatccc cttttctccc aactcatgct caatggatcg cccacgaccg gccttgatcg
      601 cctggttgcg gctcatctcc cgacctgcac cgtgacaagt ggatccaaaa cttttgagca
      661 gggcttccgg ctgtcccaga agcacatagg aggcgtctcc catgtttcca ggcacaatca
      721 ccggctgacc cagacttcga tagcagggca caacagaagg atgccctgca gggaaacacc
      781 gagtcgcacc tttccggtga acgcagagtt ccttttcgac cgatccaacg cggtgctgtt
      841 caatcttggc gatgttatgg gagacatcat agacaagagt catcccgagt ttctgccatc
      901 ctctgccaaa gatctgttcg aacgtctccc tgacccaatg catgatgcac tgacgattgg
      961 caagggcgaa gttcgccgct gcattcatgg ttttgaggta ctcctgcccc tcatcggaag
     1021 acaccggcgc acacaccagc tcggcatccg gaagatcaaa cttgaacttg ttgcgagaac
     1081 gtcgcatcag attgattgag tccgtgcatg cctgatgtcc cagaccacgc gatccgcagt
     1141 gaatcattac ggtgacttgc cctttttgaa gaccaagcgc ttcagcagct ttggcatcgt
     1201 agacttcgtc cacaacctgg atttcgagaa agtgatttcc cgcgccgaga gtgcccaact
     1261 gatcgcgccc gcgtttgcgc gcttctttgg aaaccagtgc cggatcggc
//
LOCUS       DAOQUC010001349         1309 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_146061_length_1309_cov_2.992026, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001349 DAOQUC010000000
VERSION     DAOQUC010001349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1309
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_146061_length_1309_cov_2.992026"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="PKH07_18220"
     CDS             <1..208
                     /locus_tag="PKH07_18220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007412788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNT36931.1"
                     /translation="LPDGVSAGGHGGSHGYLSHEFIMSILENRKPLIDVAWAMNMTVP
                     GIVAHQSALKDGELLKIPQYEFPG"
     gene            268..1101
                     /locus_tag="PKH07_18225"
     CDS             268..1101
                     /locus_tag="PKH07_18225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36932.1"
                     /translation="MNDPIETNANPFPLHKLEPLFNDLQLPTEPFDPQGTWKHTYQCY
                     TIAKNMMHNGTLAIYRSPSNNGRILLQIEFQKKLLQGDLQVLRAKMVCRCDDLTTPMH
                     WSFDVKTLGQSDDRCEPFDISKTAVLKEGHIEIAYQGNKRHLNLPKSYSSEWSLFDAV
                     QRLKADRNVVLQFNLLEDFDRFKPEQELSFIESQQISLGQTTAQEPNRPRRRQPVHSG
                     NATIEVEMFRQLGRGISPTFYWRDSFGRLLFVVAGIEAYVLSTHALGIPERKTRRNEQ
                     I"
     gene            1088..>1309
                     /locus_tag="PKH07_18230"
     CDS             1088..>1309
                     /locus_tag="PKH07_18230"
                     /inference="COORDINATES: protein motif:HMM:TIGR01409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HNT36933.1"
                     /translation="MNKSDARTATCKDGFTRRDFLKTSMLAGVGTLAVGCKTLSRTSS
                     TRTPERPNIVLVLVDQLSLDAISAHGCPYA"
BASE COUNT          316 a          313 c          361 g          319 t
ORIGIN      
        1 ccttccggac ggagtctccg caggtggaca cggcggatcg catggctatc tcagccatga
       61 attcatcatg tccattctgg agaatcggaa accgctgatt gatgttgcct gggcgatgaa
      121 tatgaccgtt ccgggaattg ttgcccatca gtcggcactc aaagatgggg aactgctcaa
      181 gattcctcag tatgagttcc ccggatagag tgatcttcag tttctgcggg atgccggctc
      241 tttagtggca gagtgaagga gggtcctgtg aacgatccga ttgagacgaa tgccaatccg
      301 tttcctttgc acaaattgga accgctgttc aacgatctgc aactgccgac ggagccgttt
      361 gatccgcagg gcacatggaa gcacacctat caatgctaca cgatagccaa gaacatgatg
      421 cacaacggaa cgctggccat ttaccgttcc cctagtaaca atgggaggat tctgcttcaa
      481 atcgagtttc agaagaagct gctgcaaggg gatcttcagg tgctgcgtgc taagatggtg
      541 tgtcgatgtg atgacctgac gacgcccatg cactggtctt ttgatgtgaa gacgctcggt
      601 cagtctgatg atcgatgtga accgtttgat atttcaaaaa cggctgttct caaagagggg
      661 cacatcgaga ttgcgtatca gggaaacaag cgacatctga accttccgaa atcctacagc
      721 agtgaatggt cgctttttga tgctgttcag cggttgaaag cggatcgcaa tgtcgtcttg
      781 cagtttaatc tcctcgaaga tttcgatcgg ttcaaacccg agcaggaatt gtctttcatt
      841 gagtcgcagc aaatcagtct tggacagacg acggcgcagg agccgaatcg tcccaggcgc
      901 agacagccgg tgcactcagg gaatgccacg attgaagtgg agatgtttcg gcaacttggg
      961 cgtgggattt caccaacctt ctattggaga gattccttcg gtcgtttgct gttcgttgtt
     1021 gcagggattg aggcctatgt gctcagtact catgcgcttg ggattcccga gagaaagacc
     1081 agaagaaatg aacaaatctg acgctcgtac agcaacctgc aaggatggat tcacccgaag
     1141 ggattttctc aaaaccagca tgcttgcggg agtgggaact cttgcagtgg gatgcaagac
     1201 actgagccgg acttccagta ctcggactcc ggaaaggccg aacattgtcc tggtacttgt
     1261 cgatcagctg agtctcgatg ccatatccgc ccatggttgt ccctatgcg
//
LOCUS       DAOQUC010001350         1307 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_146353_length_1307_cov_3.944089, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001350 DAOQUC010000000
VERSION     DAOQUC010001350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1307
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_146353_length_1307_cov_3.944089"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..247
                     /locus_tag="PKH07_18235"
     CDS             <1..247
                     /locus_tag="PKH07_18235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36934.1"
                     /translation="TLYRKFGDVISFCGGIDVRTLISNDRSLIDEELTRKITPVIKGG
                     GGYILHTDHSEPPEINYETMRYFIERAQQVANDAKAS"
     gene            complement(382..603)
                     /locus_tag="PKH07_18240"
     CDS             complement(382..603)
                     /locus_tag="PKH07_18240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013787627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HNT36935.1"
                     /translation="MLEKVKSALEEIRPKLQADGGDIELVDVQDDGTVNVRLKGACSG
                     CPFAVQTLKQGVEVFLKERVPSVKTVKSV"
     gene            complement(682..>1307)
                     /locus_tag="PKH07_18245"
     CDS             complement(682..>1307)
                     /locus_tag="PKH07_18245"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012414518.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     pyruvate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HNT36936.1"
                     /translation="PEVTECQVRAILEATAELIKEKKKAFPEIMIPVTCTKNELEDQK
                     ALVNRIHAEVCKKFNIKSVPHLYGTMIEIPRAALTANEMAETAEFFSFGTNDLTQMGF
                     GFSRDDIGGFLPDYLSKKILADDPFQTIDQVGVGQLIQYGIERGRATRPNLKVGICGE
                     HGGDYESVKFCHRVNMTYVSCSPFRIPIARLAAAHASIEDKKASKKK"
BASE COUNT          304 a          380 c          318 g          305 t
ORIGIN      
        1 gacgctttat cggaaattcg gtgacgtgat ttctttctgc ggcggaatag atgttcgcac
       61 actgatctcc aacgaccgct cgctcataga tgaggaactc accagaaaga tcacgccggt
      121 catcaaaggc ggcggcggtt acatccttca cacggatcat tcagagccac ccgaaatcaa
      181 ctatgagacc atgcggtatt tcatcgaacg tgcgcagcaa gtggcaaatg acgcgaaagc
      241 atcttagctg atagtcagaa accagagaga gacgctttcc gtccgagcaa gtgtcacccc
      301 ccatcgcgga caaagcacaa cggggtcaag cccatcagtg gacttgaccc cgtgatcctc
      361 tgttgagacg ctcgcctgag cctagacgga cttcaccgtc ttgacagaag gcacccgctc
      421 tttcaggaag acttcgacgc cctgcttcag agtctgaacc gcgaaaggac atccggaaca
      481 cgcacccttg aggcgcacgt tgaccgttcc atcatcctga acgtcaacca gttcgatgtc
      541 tccaccgtct gcctgaagtt tcggccggat ttcttccaac gccgatttga ccttctccag
      601 catcgtagtt gtttctgtca tgatactcct tgcgaggaat ggctccgtgt tcatgaaaca
      661 acacggagcc gtccttcgaa cttacttctt cttcgaggcc ttcttatcct cgatggatgc
      721 atgggctgcc gccagtcgtg caatcggaat ccggaatgga gaacaggaca cgtacgtcat
      781 gttcacacga tggcaaaact tgacggactc atagtcgcca ccatgctcac cgcagattcc
      841 aaccttcaga ttcggacgag ttgcccgacc gcgctcgata ccatactgga tcaactgacc
      901 cacacccacc tgatcgattg tctggaacgg atcgtctgcc agtatcttct tgctgagata
      961 gtcgggcagg aagccgccga tatcgtcacg ggagaagcca aagcccatct gcgtcaagtc
     1021 attggttccg aagctgaaga actcagccgt ctcagccatc tcgttggctg tcaaggccgc
     1081 gcggggaatc tcaatcatcg ttccgtaaag atgcgggacg gattttatgt tgaacttctt
     1141 gcagacttcc gcgtgaatcc gattcaccag cgccttctga tcttcgagct cattcttggt
     1201 gcaggtgacc ggaatcatga tctcgggaaa agcctttttc ttttccttga tcaactcggc
     1261 cgtcgcttcc agaatggctc gaacctgaca ttcggtcact tcgggat
//
LOCUS       DAOQUC010001351         1307 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_146392_length_1307_cov_2.835463, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001351 DAOQUC010000000
VERSION     DAOQUC010001351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1307
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_146392_length_1307_cov_2.835463"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..424
                     /locus_tag="PKH07_18250"
     CDS             <1..424
                     /locus_tag="PKH07_18250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36937.1"
                     /translation="SGLHSEVFNHGRMVALGVNVEMSNDFEGTEPTQVIGVNIQATHG
                     KRPMQYGMQIHTMENGNYFEKALGLNGIGDVGIEMNGTYKVGINAQNNTIRVNEGTCI
                     ELDGKGAIRLRYKDGRIEFLNGDKCFGHLDVNGEDHAL"
     gene            474..>1307
                     /locus_tag="PKH07_18255"
     CDS             474..>1307
                     /locus_tag="PKH07_18255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36938.1"
                     /translation="MFTLAVSVHCIEPVAEGILSGQRVEQDRAAKRQLLQDTRFTCGV
                     QHGIGNTYPDDLDEACRARWMKRVPSLAHAKWRFTEIAEKTYFAENPDTPTRNNALTY
                     ASPDASKQFIIAPDGLVRFVFDSAKEWRQGCRLDLPDEKGTMPRFGSPNWNWPHFLIN
                     QVIVDASDESHKVHLEKYDDIILSATVTLRDIAKTGQDCGDQVPDHAIFYFAFVLENQ
                     KGESPHTFYALVNALYTEDGSTHVSVAPWLGNDPINAAVFFCPGYPALAQGETTDYHI
                     SV"
BASE COUNT          343 a          334 c          354 g          276 t
ORIGIN      
        1 gtccggtctg cattcagagg tgttcaacca cggccgaatg gttgctctcg gtgtcaatgt
       61 tgagatgagc aacgactttg agggcaccga acccacccag gtcatcggag tgaacattca
      121 ggcgacgcac ggcaaacgcc ccatgcagta cggaatgcag attcacacga tggagaacgg
      181 aaactacttc gagaaagccc ttggtttgaa cggcatcggt gatgtgggaa tcgagatgaa
      241 cggcacctat aaagtcggaa tcaatgcaca gaataacacc attcgagtca acgaaggaac
      301 gtgcatcgaa ctggatggca agggagccat tcgacttcgg tacaaagatg ggagaatcga
      361 gtttctcaac ggagacaaat gctttgggca tctggacgtc aatggtgagg atcatgcgct
      421 gtaaaacggg cttcttgaga agatcggcag cgagtctctc tgcagtagga ctgatgttca
      481 cgcttgcagt gagcgtccat tgcattgagc cggttgccga aggaattctc tcaggacaaa
      541 gggtggaaca agatcgggca gcaaaaagac aactcctgca ggacaccaga ttcacctgcg
      601 gggttcagca cggtatcggt aatacctatc ccgatgatct ggatgaagca tgtcgggctc
      661 gctggatgaa gagagtccct tcgttggcac acgccaagtg gcgttttaca gagatcgctg
      721 aaaagacata cttcgcagaa aacccggaca ctccaacccg gaacaatgcg ctgacctatg
      781 cctctccgga tgcttccaag cagttcatca tcgcacctga tgggttggtt cgatttgtct
      841 ttgacagcgc caaagagtgg cggcaaggat gccggctgga cttacccgat gagaagggaa
      901 ccatgcctcg ctttggcagt ccgaactgga actggcctca ctttctcatc aaccaagtca
      961 tcgtagacgc cagtgatgag tcacacaaag tacatctgga gaagtacgac gacatcatct
     1021 tgagtgcaac ggtcaccctg agagacatag ccaagacggg tcaggactgt ggtgatcagg
     1081 ttccagacca cgcaatcttc tactttgcct tcgtgctgga gaatcagaaa ggtgaatccc
     1141 cgcacacctt ctacgccctg gtcaatgccc tctatacgga agatggatcc acacacgtca
     1201 gcgttgcccc ttggcttgga aatgacccta tcaatgccgc agttttcttc tgtcctggat
     1261 atcccgcact ggcgcagggt gaaacgacgg actaccatat cagcgtg
//
LOCUS       DAOQUC010001352         1304 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_146933_length_1304_cov_3.280224, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001352 DAOQUC010000000
VERSION     DAOQUC010001352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1304)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1304)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1304
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_146933_length_1304_cov_3.280224"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1237
                     /gene="rsxC"
                     /locus_tag="PKH07_18260"
     CDS             <1..1237
                     /gene="rsxC"
                     /locus_tag="PKH07_18260"
                     /inference="COORDINATES: protein motif:HMM:TIGR01945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HNT36939.1"
                     /translation="PTPSQLLIPFRQHIGAPCRPVINRGDSVVYGQLLAEEGGFVSTR
                     IHAAATGRIKLLNLRWHVNGRKELTAELDVAEDPGIPRTPDAVDWSVLDRLERKEILS
                     RIQEAGIVGMGGAGFPTHVKLQPPETKPIEIAILNGCECEPYLNADNRLMIERSELSV
                     FGFKAVMKVLGAKTGIIAIESNKPEAASLMKSACKGHNDLTISILQTKYPQGGEKMTI
                     LAATGRRVPEGGLPMDVGTVVSNIGTSVAIAEAVLWNRPLTHRVLTVAGSGVKKPGNL
                     LVPIGATIGNCIEFCGGLSPDACALIIGGPMMGLAITDLDVPVIKTTSGILVLSKEEY
                     GLPDESACIRCARCVDRCPLSLLPSQIYRYAQRQDWDVLKKDLHLLNCMECGSCAYVC
                     PAGIPLVQHIRVAKALLPK"
BASE COUNT          285 a          346 c          368 g          305 t
ORIGIN      
        1 ccccactccg agccaacttc tcatcccttt tcgccagcat atcggagcgc cgtgccgacc
       61 ggtgatcaat cgcggagaca gcgttgtcta tgggcaactg ctcgccgaag aggggggctt
      121 tgtctccaca cggattcatg ccgctgccac tggccggatc aaactgctga atctgcgctg
      181 gcatgtgaat ggccgcaagg agttgaccgc tgaactcgac gttgccgagg atccaggcat
      241 tccgcgaaca cccgacgcag tggactggtc ggttctggat cggctggaac ggaaggagat
      301 tctctctcgc atccaggaag cgggcattgt cggtatgggt ggggctggtt tccccactca
      361 tgtcaagctt cagccacccg agaccaagcc catcgaaata gccattctga acggatgcga
      421 atgtgaaccc tatctgaatg cggacaacag gctcatgatc gagcgttccg agctgagtgt
      481 tttcgggttc aaggcggtta tgaaggtact cggtgccaaa accggcatca ttgccattga
      541 gtccaacaag cccgaggcgg caagtctgat gaaatccgcc tgcaagggac acaacgatct
      601 tacgatttcc atacttcaga ccaaataccc gcagggcgga gagaagatga cgattctcgc
      661 cgctacgggg agacgggttc ctgaaggtgg actgccaatg gatgttggca ctgtggtaag
      721 caacattgga acgtcggttg ccatcgcgga agcggttttg tggaaccgtc cgttgactca
      781 tcgagttttg accgttgctg gttctggtgt caagaagcct gggaatcttc tcgttcccat
      841 cggagccacc atcggcaact gcattgaatt ctgcggcgga ctttctccag atgcctgtgc
      901 gctgatcatc ggcggaccga tgatgggctt ggcgatcacg gatctggatg ttccagtgat
      961 caaaacaaca tccggcattc ttgttctctc caaagaggaa tacggattgc ccgatgagtc
     1021 cgcctgcatt cgctgtgcac ggtgtgttga tcgctgtccg ttgtctttgt tgccgagcca
     1081 gatctaccga tatgcgcaga gacaggactg ggacgttttg aaaaaagatc tccacttgtt
     1141 gaactgcatg gagtgtggca gctgcgctta tgtctgccca gccggcattc cattggttca
     1201 gcacattcgg gttgcgaaag cattgttgcc aaaatgagtg atatcgagaa agtatctcaa
     1261 ttcatcctca agtcttcgcc acacgcccat tcgggtttga cgac
//
LOCUS       DAOQUC010001353         1302 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_147327_length_1302_cov_2.855654, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001353 DAOQUC010000000
VERSION     DAOQUC010001353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1302
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_147327_length_1302_cov_2.855654"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..254
                     /locus_tag="PKH07_18265"
     CDS             <1..254
                     /locus_tag="PKH07_18265"
                     /inference="COORDINATES: protein motif:HMM:NF016489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HNT36940.1"
                     /translation="MANKVTLDEVAGFLVTMFAIDPTLKTVAAGFFLFRFFDVAKLFP
                     ARLVERKAPGGWGITFDDIIAGLWAQLILRLLLHFGLLA"
     gene            complement(307..>1302)
                     /locus_tag="PKH07_18270"
     CDS             complement(307..>1302)
                     /locus_tag="PKH07_18270"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HNT36941.1"
                     /translation="QHGPGSEGCWVILDQQGKCNKNEYFWFSPGVPAVHEHARKVAVD
                     LCKRYDLDGIHLDRIRYPSAEDYNPTAVARFNVSGNPMGLDRLNWQLAEMNRMVANLS
                     SAVWAIKPEMKMTAAVWGIYDKTKIPGYGGFSTGLQQYCQDSLAWARLGLVDALCPMI
                     YWDLPDPKPNYDECLKQFLEGRGNRHVYGGFHAKFEPAEIMAQIEATRKLGGQGTVAF
                     STTSVSKAPRWEYFRTQIYPAKVKTPEMTWKTKPTEGSIVGYVKRSTDEPVVDAYVTI
                     EGQDGGVLSCADGYFSFLWLKPGKYTVTARIGGRQAKVADLEVKAGEFAVANLTI"
BASE COUNT          286 a          386 c          311 g          319 t
ORIGIN      
        1 ctatggcgaa caaagtcacc ttggatgaag tggctgggtt tctcgtcact atgttcgcca
       61 tagacccgac actgaagact gtcgccgccg gattctttct cttccgtttc ttcgatgtag
      121 ccaaactttt tccggcacgc ctggtggaga gaaaagcacc cggtgggtgg ggaatcacgt
      181 tcgatgatat cattgccgga ctgtgggcac agttgatctt acgactgctt ctgcacttcg
      241 gcctgctcgc atagaccact tgcctgcagg ttcagacctg ctcaatcgtc gtcggtcgcg
      301 accagatcat attgtcagat tcgccactgc gaattcaccg gctttgacct ctagatcagc
      361 cactttggcc tgtcgtccgc cgattctggc cgtcactgtg tacttgccag gcttcaacca
      421 caagaaggag aagtacccgt cagcacaaga caggacaccc ccgtcctgtc cctcaatcgt
      481 gacataggcg tcaaccacag gttcatctgt agacctcttc acgtatccaa caatacttcc
      541 ttcggtcggt ttcgtcttcc aggtcatctc gggcgtcttc actttggcag gatagatctg
      601 tgtccggaag tactcccatc gaggtgcctt tgagacagat gtggtggaaa aagcgaccgt
      661 accctgccca cccagttttc gggttgcctc aatctgtgcc atgatttccg cgggttcaaa
      721 cttcgcatga aaacccccat agacatgcct gttgcctcga ccctcaagaa actgtttgag
      781 gcattcatca tagttcggct tgggatccgg cagatcccag tagatcattg gacacaaggc
      841 gtccaccaac ccaagacgcg cccatgccag tgaatcctgg caatactgct gaagcccagt
      901 cgagaagcct ccgtagccag ggattttagt cttgtcgtag atgccccaaa cagccgcggt
      961 catcttcatc tctggcttga tcgcccagac cgctgatgag agattcgcaa ccatcctgtt
     1021 catttccgcc aattgccagt tgagtcggtc gagtcccatg ggattacccg aaacattaaa
     1081 gcgcgccacg gcggtcggat tgtagtcctc tgcactcggg taacgaatcc tgtccagatg
     1141 aatcccatca aggtcatacc tcttgcagag atcaacggca accttgcgag catgctcgtg
     1201 aaccgcagga actcctggac tgaaccagaa atactcgttc ttgttgcatt taccctgttg
     1261 atccaggatc acccaacagc cttctgaacc cggaccgtgc tg
//
LOCUS       DAOQUC010001354         1302 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_147328_length_1302_cov_2.833200, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001354 DAOQUC010000000
VERSION     DAOQUC010001354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1302
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_147328_length_1302_cov_2.833200"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..652)
                     /locus_tag="PKH07_18275"
     CDS             complement(<1..652)
                     /locus_tag="PKH07_18275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36942.1"
                     /translation="MKRTAERRLSWVAFCLVLALAPASVWPQAYYASKDRNLVIVSDV
                     QVKRSLEVTDTAVDYAYGALAPSGQYVIGSGLIERKSSYQPIEGTTLYLVSQDGKTRK
                     KIAEDVLDAFPASRLNRLAFITRDFRLHIFQDGKVTTASFPNTTNYVSWLPDADRLVL
                     TGRDADWSYDAMSSPRDTEEFLRMNTSNLFLYDISSDTLTALTDNDQANYNPVAAPD"
     gene            complement(812..>1302)
                     /locus_tag="PKH07_18280"
     CDS             complement(812..>1302)
                     /locus_tag="PKH07_18280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36943.1"
                     /translation="PSWESTRAAIFMKTDSRQPDSWLLEDVQLQCEMAAETLKKAIAL
                     GKTIRVPDTLSEDFNQTLTDLDGWRKRTLSYAYHLRETNLARMMRMHSEKGQEIPEKM
                     LSEMRAILQADLENQDGAEPCRKALELLEKDLAEFLRTYFVVPEPATDPHSLGPFTLT
                     SR"
BASE COUNT          306 a          356 c          326 g          314 t
ORIGIN      
        1 catcaggagc agccactgga ttgtagttcg cctgatcgtt gtccgtcaga gccgtaagcg
       61 tgtcgcttga aatgtcatag aggaacaggt tgctcgtgtt catccgaaga aattcctcgg
      121 tatcacgagg ggaagacatc gcatcgtaag accagtcggc atctctgccg gtgagcacca
      181 gacgatccgc atcgggcagc catgaaacat agttggtcgt attcggaaag gatgccgttg
      241 tgaccttgcc atcttgaaag atgtgaagtc gaaagtctcg ggtgataaac gccagtcggt
      301 tcagtcttga tgccggaaaa gcatccagaa catcctcggc gatcttcttt cgcgtcttac
      361 cgtcctggga gacaagatag agtgtggtgc cttctatggg ctggtaggat gacttgcgtt
      421 cgatgagccc ggaaccaatg acatactggc cactgggggc aagagcgcca taggcatagt
      481 caacagccgt gtccgtaact tcaagcgaac gctttacctg aacgtcggag acgataacga
      541 gattcctgtc cttggaggcg tagtaggctt gaggccagac agatgcaggc gcaagagcca
      601 acacaagaca aaacgcaacc caagagagtc ttctttccgc agtcctcttc acggttcccg
      661 tacctcctcc tcgtcagccc tttgcacctc ccctcggcca cacgtctatt tcctggcaat
      721 atagcccagc ctgcctggtc tgtcacccct gggaattgct ttgttccatc aagatcactc
      781 ctggagcgca gctgttctgc catcaagcct ctcagcgaga ggtcagtgtg aaaggaccaa
      841 ggctatgcgg atctgtcgct ggttccggaa caacaaaata agttcggaga aactcggcaa
      901 gatctttctc aagaagctcc aaagcttttc ggcaaggttc cgctccgtct tggttttcca
      961 gatcggcttg aagaatcgcc ctcatctcgc tcagcatttt ctcaggaatc tcctgccctt
     1021 tctcagaatg catccgcatc atccgagcaa gattagtttc acgaagatga taggcgtaag
     1081 agagagtcct ctttctccat ccatcaagat cggtgagagt ctggttgaaa tcttctgaca
     1141 acgtgtcagg aactcgaatt gtcttcccca gagcgatggc tttcttcaaa gtctccgctg
     1201 ccatctcaca ctgcaattga acatcttcca acagccaact atccggctgt cggctatcgg
     1261 tcttcatgaa gatcgctgcc cgtgtggatt cccaactggg cg
//
LOCUS       DAOQUC010001355         1301 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_147495_length_1301_cov_3.139647, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001355 DAOQUC010000000
VERSION     DAOQUC010001355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1301
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_147495_length_1301_cov_3.139647"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..740
                     /locus_tag="PKH07_18285"
     CDS             <1..740
                     /locus_tag="PKH07_18285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36944.1"
                     /translation="TKSSHKNILVLGIYLANTTNTVSDIVSVLSRSEKHTIVQKWVAL
                     NGQPPDEATARVTVKTLTGKRPKFQIINDLLAGDDLSRYDYVILCDDDVVLPEQFVDT
                     FIAMQSSLGLAIAQPARTLNSYIDHPIVEQHLGVHARQTLFVEIGPVVSFHTSVFDFV
                     FPFDLISPMGWGYENVWAHEIIQRGLKMGIIDAVCVDHSLRKPVANYNWEDADRQRSA
                     YLKKHDHLSLDECFKVINTYIIAEEST"
     gene            737..>1301
                     /locus_tag="PKH07_18290"
     CDS             737..>1301
                     /locus_tag="PKH07_18290"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HNT36945.1"
                     /translation="MKSVSSQPFISVIIPTYNRADLLSQSLESLVGQSLPKDQFEVIV
                     IDDGSSDNTSDVCRNFSQRLPLRYYRQKNSGISAAKNMGVFASCGPILLFFDDDDVAD
                     PDLLTEHIRTHSLHPEENIAVLGYTTWHPSLKISRVMDFVTNIGHFLFSYSNLKDGQE
                     LDFTYFWGGRSSCKKSLLVRHGVFNQLF"
BASE COUNT          331 a          355 c          302 g          313 t
ORIGIN      
        1 taacaaaatc gtcgcacaag aatattctcg ttctcggaat ctatcttgcg aacactacaa
       61 acacggtcag cgacatcgtt tccgtcctca gccgttcgga aaaacacaca atcgtacaga
      121 aatgggtcgc actcaacggg caacctcccg atgaggcaac ggcaagggtc accgtgaaaa
      181 cattgaccgg caagaggccg aagtttcaga tcatcaacga tctgcttgca ggagacgatc
      241 tatcgcgcta cgattatgtc atcctttgcg atgacgacgt ggtccttccg gaacaattcg
      301 tggacacttt catcgcaatg caatcttcgc ttgggttggc gatagcccag cctgccagaa
      361 ctctcaactc gtacattgac catcccatcg tggagcagca cctcggagtt cacgccaggc
      421 agactctatt cgttgaaata ggaccggtgg tcagttttca cacttcagtc tttgacttcg
      481 tctttccttt cgacctcatc tcacccatgg gatggggata cgaaaatgtc tgggcacacg
      541 aaatcattca aaggggtctt aagatgggta tcatcgatgc ggtctgcgtt gatcacagtc
      601 tgcgcaaacc ggtggccaat tataactggg aagatgccga tagacagaga agcgcatatc
      661 tcaagaagca tgatcacctt tcgctggacg agtgcttcaa agtcataaac acctatatca
      721 tcgccgagga gtcgacatga aatctgtatc ctctcagcct ttcatcagcg tcataatacc
      781 cacatacaat cgggccgact tgctgagcca atccctggaa agcctggtcg gacagagtct
      841 gccgaaagat cagttcgagg tgatcgtcat tgacgatggt tcttctgaca acacgtcaga
      901 tgtatgcagg aatttttccc aaaggctccc cttgcggtac taccggcaga aaaactctgg
      961 tatttcagct gcaaagaaca tgggtgtgtt tgcttcctgc ggcccgatcc tcttgttctt
     1021 tgatgacgac gacgtcgccg atcccgacct gctcacagag cacatccgca cccactccct
     1081 tcacccggaa gaaaatatcg cggtgctggg ctataccaca tggcaccctt cactgaaaat
     1141 aagccgagta atggatttcg tcacgaatat cggccacttc cttttctcct acagcaacct
     1201 taaagacggt caggagctgg atttcacgta tttctggggc gggcgttcat catgcaagaa
     1261 gtcgctgctg gtacgtcacg gggtcttcaa ccagctcttc a
//
LOCUS       DAOQUC010001356         1299 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_147871_length_1299_cov_3.471865, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001356 DAOQUC010000000
VERSION     DAOQUC010001356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1299
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_147871_length_1299_cov_3.471865"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..788
                     /locus_tag="PKH07_18295"
     CDS             <1..788
                     /locus_tag="PKH07_18295"
                     /inference="COORDINATES: protein motif:HMM:NF037272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lectin like domain-containing protein"
                     /protein_id="HNT36946.1"
                     /translation="GLATTASAVTNHAVTIVGWDDNQPYDRGHDGSIDGQGCWIVKNS
                     WGTGWGGTCGYGTEKGYFYIAYANAEIGTWASYIISHQNYDTSGGLLSFDDAGWGNLN
                     WGYSNTTCWALCRYAAPSNTQATAIEFWTNDVTTDVDVYLYNGFNPATTTLGTLLASS
                     LDNAFPEAGYHAVSITPTNIGPASNPEAVAVVKITNSTYTSPIPADYGNGSPPLETNR
                     CYISPNGNNGSWFQTSTHTTYPSDLGVRIRTHNPPAVNEWMQY"
     gene            complement(948..>1299)
                     /locus_tag="PKH07_18300"
     CDS             complement(948..>1299)
                     /locus_tag="PKH07_18300"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HNT36947.1"
                     /translation="QEELAPFKAPFDFTEWDIKSGRYVLITDKEKKLEIAKQISPITH
                     VTEDDPPTYIIHGDKDPIVPIQQSESMKAALEAVKVPVVLDIRKGQGHGWEGWLDDLG
                     LFAEWFDQHLQKAK"
BASE COUNT          299 a          355 c          342 g          303 t
ORIGIN      
        1 atgggcttgc caccacagca tctgctgtca ccaaccatgc ggtcacgatc gttggatggg
       61 acgataacca gccctatgat cgaggccatg acggcagcat tgacgggcaa ggttgctgga
      121 tcgtcaagaa cagctgggga acagggtggg gtggcacctg tggatatgga acggagaagg
      181 gctacttcta cattgcctat gccaatgccg agattggcac ctgggcttcc tacatcatca
      241 gccatcagaa ctacgacacc tccggtggac tgctctcctt tgacgatgcg ggttggggaa
      301 atctcaactg gggttattcg aacacaacct gttgggcttt gtgccgctat gcagcgccat
      361 ctaacacaca ggcaacagcc attgagttct ggacaaatga tgtcacgaca gacgtagacg
      421 tctatctcta caatggcttc aatcctgcaa ccacaacatt gggaacgctc ctggccagct
      481 ctctggacaa cgcattcccc gaagcaggct accacgctgt cagcatcact ccgacgaaca
      541 ttggacccgc gtccaatcca gaagctgttg ctgttgtcaa gatcaccaat agcacttaca
      601 cgtcacctat ccctgcagac tacgggaatg gttccccgcc acttgagacc aatcgctgtt
      661 acatcagccc caacgggaac aatggcagct ggttccagac gagcactcac acgacgtacc
      721 cgagtgacct gggcgtcagg attcggacac acaatccgcc ggcggtcaat gaatggatgc
      781 agtactaagc agtctggcac ctgcgggcgt tcgggttatg cccactctct tggatgcctg
      841 ctggaaggct gacaagtccc cttggactga gtgtcattga gaggtagata ccttacggtt
      901 gattcagttg aagtgaagaa ctgctcccga agttgcgaga cggttcttca cttcgctttt
      961 tgaaggtgct ggtcaaacca ttcggcaaac agcccgagat cgtccagcca accttcccag
     1021 ccgtgtccct gacccttgcg gatgtcgagc acgacaggga ctttcaccgc ttccaaggcg
     1081 gccttcatgg attcagactg ttgaatggga acaatgggat ccttgtcacc gtggataata
     1141 taggttggag gatcgtcttc ggtgacatgt gttattggcg aaatctgttt ggcgatctcc
     1201 agtttcttct ccttgtccgt gatcaaaacg tatctgccgc ttttgatgtc ccattcggtg
     1261 aagtcaaaag gcgctttgaa gggtgccagt tcttcctgg
//
LOCUS       DAOQUC010001357         1299 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_147888_length_1299_cov_3.091640, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001357 DAOQUC010000000
VERSION     DAOQUC010001357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1299
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_147888_length_1299_cov_3.091640"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(77..>1299)
                     /locus_tag="PKH07_18305"
     CDS             complement(77..>1299)
                     /locus_tag="PKH07_18305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36948.1"
                     /translation="PTLLAPFVKVFGEAYWYGRLLSTLGCLATALTVGLLVFGATGRK
                     MPSLLAALSLLSFYHYYEWGAYCRVDALAVAFVAVGLLLVDRGFPVCAYLPLFVAALF
                     TRQTMIAAPLAVIAMLWSTERRSEARRFALGLVCCVGVIGLALNSLTQGEFLLHTVVY
                     NANLFSIRDMFLYLLHLARYSGFYCAFGLTYFIFVVSEGRRSLALYFLIFSFLAGLMC
                     GKVGAAPNYWFEFIVALCWTLGLLWAECHEVQGRGGRVLSVILPVFMLLQIVANFHVP
                     HTRYDYSQTPTQESKAQQAMLLDYVKAVQGSVLCQDGGLNLLAGKEIPYQPFEFSQLL
                     YQGIWDESPILDALVAKQFDLLVLPFDLSTANLSQERFTPGFIQTARDNYGLVKRVGT
                     SFVHTPSKKNVFMD"
BASE COUNT          363 a          348 c          338 g          250 t
ORIGIN      
        1 gatggggaca tttctatttt gcagaaaaag ggacatttct actttgcgtt gacagagcga
       61 agggaattcg ttgtccctag tccatgaaca cattcttctt cgaaggtgtg tggacgaatg
      121 acgtgccgac tcttttgacg agaccgtagt tgtcgcgcgc tgtctggata aacccagggg
      181 tgaagcgttc ctgactgagg ttggctgtag acaaatcgaa aggcagcaca agcagatcaa
      241 actgttttgc gacaagcgcg tccaggattg ggctttcatc ccagattccc tgatagagca
      301 gttgactgaa ttcaaagggc tgatagggga tttcctttcc tgccaagagg ttcagccctc
      361 cgtcctgaca cagaacggag ccttgtaccg ctttcacgta atcaaggagc atcgcctgct
      421 gtgcctttga ctcctgtgtt ggcgtctgcg agtagtcata ccgagtatgg ggaacatgaa
      481 agttcgccac gatctgaagc agcatgaaca ccggcagaat cacgctcaag actcgaccgc
      541 ctctcccttg aacttcgtgg cattccgccc agaggagtcc gagtgtccaa cagagggcaa
      601 cgatgaactc aaaccagtag tttggcgcag caccgacctt accgcacatc aaacccgcca
      661 gaaaggagaa gatgaggaag tacagagcaa gacttcgccg accttcgctg accacgaaga
      721 taaagtacgt cagaccgaag gcacagtaga atcccgaata gcgcgccaga tggagcagat
      781 agagaaacat atcccgaatg ctgaacaggt tggcattgta gacaaccgta tgcaggagaa
      841 attctccctg ggtcagggag ttgagcgcca aacctatcac cccgacacag cacacaagcc
      901 ccaaggcaaa tcgccgtgcc tcagaacgtc gttctgtact ccagagcatg gcaatcacag
      961 ccaatggtgc tgcaatcatg gtctgacggg tgaacaacgc tgcaacaaaa agcggcagat
     1021 acgcgcagac aggaaatccc ctgtccacca aaagcagccc cacagcgaca aaggcaactg
     1081 cgagagcatc cacgcggcaa tacgctcccc actcgtagta gtgatagaac gacagaagcg
     1141 aaagggcagc cagcaggctt ggcattttgc gtcccgtggc tccaaagacc agcagtccga
     1201 ctgtcaaagc cgttgccaga caaccaagtg ttgaaagaag tctgccatac caataggctt
     1261 ctccgaacac cttcacgaaa ggcgctaaga gtgtcggaa
//
LOCUS       DAOQUC010001358         1297 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_148274_length_1297_cov_2.800322, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001358 DAOQUC010000000
VERSION     DAOQUC010001358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1297)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1297)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1297
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_148274_length_1297_cov_2.800322"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..432
                     /locus_tag="PKH07_18310"
     CDS             <1..432
                     /locus_tag="PKH07_18310"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNT36949.1"
                     /translation="GGIGGGLMGIGERMTWLAIASLIVCMVGISNAMLMSVTERFREI
                     ATLKCLGALDSFIMLTFVLEACCLGVVGAFVGALAGAVIGLLRMAVSFGGLLLSAIPL
                     QPMALAIALSVLLGVILAAVASVYPSLKAARLAPMEAMRIE"
     gene            522..1208
                     /locus_tag="PKH07_18315"
     CDS             522..1208
                     /locus_tag="PKH07_18315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005983609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNT36950.1"
                     /translation="MNNVVTEALRGVNVEIYTGEFITVMGPSGSGKSTFFNMIGGLDS
                     PTSGRVFIDEVDVAQLTAVELAFLRCRKIGYIFQDYNLIQYMTALENVTTPMAFGGVP
                     PDEARRRGMELLELVGLAERWFHRPIEMSGGQQQRVAIARSLANNPRILLCDEPTANL
                     DLRTGREILEILRKLNKQRGVTIICATHDYRMMDICDRLIWIRDGNIERIARRDEVSV
                     EVGTIDNETE"
BASE COUNT          276 a          341 c          380 g          300 t
ORIGIN      
        1 ggcggcatcg ggggcggcct gatggggatc ggcgaaagga tgacctggct tgccattgcc
       61 tctctgatcg tgtgcatggt gggtatctcc aatgcaatgc tcatgtccgt caccgagaga
      121 ttccgcgaaa tcgccacact gaagtgcctc ggcgctctgg acagtttcat catgctgaca
      181 tttgtcctcg aagcctgttg cctgggagtc gtcggtgcct ttgttggagc attggccgga
      241 gctgtcatcg gcttgcttcg aatggctgtt tctttcggag gacttctctt gtcggcaatc
      301 ccgttgcagc ctatggcctt ggccatagca ctgtctgttc tgttgggggt tatcctggcg
      361 gccgtcgcct cggtctatcc gtctctcaag gctgcccgac tggcaccaat ggaggccatg
      421 cgaatagaat gatcacggag gcaacaacgg tgcaacagtc ttctgaatca acgacgcgca
      481 gagtgatcat ccgaacgctt tatctcaaac gtcattacgt gatgaataat gtggtgaccg
      541 aagccctgcg cggggtcaat gtggagatct acaccggaga gttcatcacc gtgatggggc
      601 cgtcgggttc cggcaagagc acgttcttta acatgatcgg cggacttgac agtcccacga
      661 gtggcagggt cttcatagat gaagtggatg tcgcccagtt gaccgctgtg gaactggcgt
      721 ttctccgctg cagaaagatc ggttacattt ttcaggacta caatctgatt cagtacatga
      781 ccgctctgga gaacgtcacc acgccaatgg cttttggggg agtgccccct gacgaggcca
      841 gaaggcgcgg catggaactc ctggaactcg tcggcttggc ggagcgctgg tttcacagac
      901 ccatcgagat gtctggggga caacagcagc gtgtcgccat tgcccgatct ttggcgaaca
      961 atccccgaat tctgctgtgc gacgagccaa cagccaacct tgaccttcgc acaggcaggg
     1021 agatcctcga aatcctgcgc aaactcaaca agcagcgtgg tgtcactata atctgcgcca
     1081 cacacgacta ccggatgatg gatatctgcg acagactcat ctggattcga gacggcaaca
     1141 ttgagcgcat cgcacgccgt gatgaggtga gtgtcgaggt gggaacgatt gacaatgaga
     1201 cagagtgaag tgatggaaaa cacacatcgt ttaagacagg cagcatcgct ccttcgggct
     1261 ctctgctgtc ttggtctcct gctggttgca ggaaatg
//
LOCUS       DAOQUC010001359         1296 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_148551_length_1296_cov_1.976632, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001359 DAOQUC010000000
VERSION     DAOQUC010001359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1296
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_148551_length_1296_cov_1.976632"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..539)
                     /locus_tag="PKH07_18320"
     CDS             complement(<1..539)
                     /locus_tag="PKH07_18320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36951.1"
                     /translation="MNCTGNIPFIAIHERSIPEAYETALRRVWNEGIPVRTEYDRPDD
                     PPSRDATVAIAIEDPFCQPRFHRSFADSLGALAEYVMEVVHGAHDYWVKGRSELEKGS
                     ESSDTRWTYTYHGRLCAFEMDGETTDQLDLMCSRLAKSPHTRRAQAITWNTRIDPRTD
                     DPPCLQRVWCRVVETDDNKL"
     gene            complement(705..>1296)
                     /locus_tag="PKH07_18325"
     CDS             complement(705..>1296)
                     /locus_tag="PKH07_18325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020249000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36952.1"
                     /translation="LIVVAIIGILAAIAIPNFLMAQMRAKVARAQADMQASGTAIESY
                     LVDNNVYPLDSPAPGPHTYVDDTRLPNSLTTPIAYLKNVTQMQDPFRTEDPMPAGADE
                     NYYRRYEYWDFKSTYLDYSDAFWNTRGNIYINGNGSFVGYGRWTVSSYGPDKTYGPYE
                     LGTFGIRVLYDPSNGTVSPGEIIRCQKFAQMKNNHR"
BASE COUNT          288 a          334 c          355 g          319 t
ORIGIN      
        1 agtttgttgt cgtctgtttc tacaacgcgg caccagactc tctggaggca gggcgggtcg
       61 tcggttcgag ggtctatgcg cgtgttccaa gtgatggctt gtgcacggcg ggtatgagga
      121 ctttttgcca gtctcgaaca catcagatcc agttggtctg ttgtctctcc atccatctca
      181 aaggcgcaaa gcctgccatg gtaagtgtac gtccatcgag tatcggaact ctcagatccc
      241 ttctcgagtt ccgaacgacc tttcacccag taatcgtggg cgccgtgtac cacttccatc
      301 acatattctg ccagcgcgcc aaggctgtcc gcaaaagaac gatggaagcg cggttggcag
      361 aagggatctt caatggcgat ggcgacggtg gcatctcgac tgggtggatc gtccggacgg
      421 tcatattcgg ttcggacggg gataccctcg ttccagaccc ttctgagagc cgtctcatag
      481 gcttccggaa tgctccgctc gtgtatggcg atgaaaggga tgtttccagt gcagttcatt
      541 gtcttctcaa tctcccaaag agttaccaag atcaaaggac ctcatcactg tggattgaaa
      601 atacagcgga acaagacacg aagcaagtga agaaccgttc cggtcaccct ctgaccagaa
      661 cggttctctt ctgatatgtg tcgtcaaatc ccgccccaga aaaactagcg atggttgttc
      721 ttcatctggg caaatttctg gcaacggata atctcgcccg ggctgacggt tccattgctc
      781 ggatcgtaga gaactcgaat accgaaagtg cccaactcgt agggtccata ggttttgtcg
      841 ggcccgtagc tactgactgt ccagcgtcca tagccaacga agcttccatt gccattgatg
      901 tagatgttgc ctcgtgtgtt ccagaaagcg tcggagtagt caaggtaagt gctcttgaag
      961 tcccagtatt catagcggcg gtagtagttt tcatctgcgc cggcgggcat gggatcctct
     1021 gttctgaaag gatcctgcat ctgagtgaca ttcttcaaat aggcaatagg tgtcgtcaga
     1081 gagttcggca ggcgcgtatc atctacatag gtatgaggtc cgggggccgg cgagtccaat
     1141 ggatagacgt tgttatccac cagataggac tctatagcag ttcccgatgc ctgcatatct
     1201 gcctgcgcac gcgccacctt ggctctcatc tgtgccatca ggaagttggg gatggcgatg
     1261 gctgccagga tgccgatgat cgccacaaca ataaga
//
LOCUS       DAOQUC010001360         1294 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_148866_length_1294_cov_2.619855, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001360 DAOQUC010000000
VERSION     DAOQUC010001360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_148866_length_1294_cov_2.619855"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..618
                     /locus_tag="PKH07_18330"
     CDS             <1..618
                     /locus_tag="PKH07_18330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36953.1"
                     /translation="TFWLIMVKTARAGSQLSALVICCVMFASVVPASGESPVLLAPLD
                     GVSVTLGDVLNGRVTLDWSDVPLSASYEVDIVGSLGFRNIYPYTTQSMLLFNYTSIEG
                     RYDWRVRAWFVAGSASDYSDPRSFTVTGSVRPTPSNTPTVTNTPTVTPTPVNPADRNQ
                     DGEIDTHDLFGLSALWLRSTNDGDLNGDQRFDADDLLLFIKQWHR"
     gene            complement(599..>1294)
                     /locus_tag="PKH07_18335"
     CDS             complement(599..>1294)
                     /locus_tag="PKH07_18335"
                     /inference="COORDINATES: protein motif:HMM:NF014003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HNT36954.1"
                     /translation="ITHEITPHCVSEASFLLERSYRFLPPGSVHVVIVDPGVGGSRRP
                     IAVRADKHFFVCPDNGLLTHVLAGVSEFEARLLENKDFLLVKISNSFHGRDMFAPVAA
                     YISKGVPFEKFGPRIADPVMLPLDRPFTKQDCLIGTVVYIDHYGNLVTNIREQELSGF
                     LQGSAGLVQILKHQIPRVTSSYSATPVGEPLAIIGGFGMLEIAVNCGNAQQVLGASLN
                     SEVVVSRSTDATA"
BASE COUNT          290 a          331 c          362 g          311 t
ORIGIN      
        1 acgttttggt tgataatggt taagacagcg agggcaggca gtcaattgtc tgcccttgtg
       61 atttgttgtg ttatgtttgc ctccgttgtt cctgccagcg gtgaatctcc cgtattgctt
      121 gctcctctcg atggtgtcag cgtgactctc ggcgatgttc tcaacggtcg cgtcacgctt
      181 gactggtctg acgtgccgct gtcggcatcc tatgaggtgg atattgtcgg atctctaggg
      241 tttcggaata tctatcctta cacgactcag tcaatgctgt tgttcaacta tacatctatc
      301 gaaggacgat acgactggcg tgtgcgggct tggtttgtgg ctgggtctgc cagtgactat
      361 agcgacccgc gctcctttac tgtaactggc tctgtacgac cgactccgag taacacgccg
      421 accgtgacga ataccccaac agttactccc acacccgtta accctgcaga ccgcaatcag
      481 gatggtgaga tcgatacgca cgatctgttt ggactgagtg cgctgtggct ccgatccacg
      541 aacgacggtg atttgaacgg ggatcaaaga ttcgatgcgg acgacctgct cctgttcatc
      601 aagcagtggc atcggtagac cttgagacga caacttctga attcagtgaa gcgccgagaa
      661 cctgctgagc gttgccacag ttgaccgcta tctcaagcat tccgaaaccg ccgatgattg
      721 ccaaaggctc acccacgggt gtggcgctgt aggaggaggt aactctcggt atctgatgtt
      781 tgagtatctg caccaaacca gccgagccct gaaggaatcc agagagttcc tgttcgcgaa
      841 tgttggtgac aagattgcca tagtggtcaa tgtatacaac cgtgccgatc aggcagtcct
      901 gctttgtgaa tggacggtcc agtgggagca tgacaggatc ggcaatgcgc ggtccgaact
      961 tttcaaaagg aacccctttg ctgatataag cggccactgg ggcgaacatg tccctgccat
     1021 ggaacgaatt gctgatcttg acaagaagaa agtccttgtt ctcaaggagc cgcgcctcga
     1081 attcggagac tccagccagg acatgtgtca acaaaccatt atctgggcag acgaaaaagt
     1141 gcttgtcggc tctgaccgcg atgggtcttc tcgaaccgcc gacaccagga tccacgatga
     1201 cgacgtggac gcttcccggt ggcaggaaac ggtaagagcg ttcgagcaga aacgaggctt
     1261 cggacacgca gtgcggggtg atttcatgcg tgat
//
LOCUS       DAOQUC010001361         1291 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_149377_length_1291_cov_3.329288, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001361 DAOQUC010000000
VERSION     DAOQUC010001361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1291
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_149377_length_1291_cov_3.329288"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..457)
                     /locus_tag="PKH07_18340"
     CDS             complement(<1..457)
                     /locus_tag="PKH07_18340"
                     /inference="COORDINATES: protein motif:HMM:NF014148.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HNT36955.1"
                     /translation="MVQKKIACLGAGSLYFPRALGDFALTPELSCSEIVLYDIDSEKA
                     DMMAVLGRRLVAESGVALTVRSTAGLSDALDGADFALSSIGGSGAEITPNVYDSYYHT
                     ADVYIPAKYGIPQVIGDTCGPAGMMMGLRSVPAYLAICREMEKRCPDVIV"
     gene            637..>1291
                     /locus_tag="PKH07_18345"
     CDS             637..>1291
                     /locus_tag="PKH07_18345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009324471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNT36956.1"
                     /translation="MAKKPITAIIVGAGHRSLSYAGYAKHHPDELQIVGVADLIPLRR
                     ELVARLYGLSPEQCFATAEELAQRPRMADAIINGTMDHQHVATSVPLLEAGYDMLLEK
                     PFATNEEEMWQLVNTCRKHGNKVMICHVLRYAPFYAAIRQKVIDGEIGEIINCQFAEH
                     VSYHHMAVGYVRGKWNRKDRCGSSMLIAKSCHDLDLMTWMKSGIEPVRVSSFGSNMQF
                     "
BASE COUNT          330 a          359 c          311 g          291 t
ORIGIN      
        1 atacgatgac gtccggacat cgcttctcca tctcacgaca gattgccaga tatgcgggta
       61 cagagcgaag ccccatcatc atgccagccg gaccgcatgt gtccccaatg acctgcggga
      121 ttccatactt ggctggaata taaacatcgg ctgtgtgata gtaggaatca tagacgttcg
      181 gagtgatttc agcaccgctc ccaccgatgg acgacaaggc aaaatcggct ccatctaatg
      241 catcactgag tccagctgtt gaacgaacgg tcaacgccac acccgactcg gcgacaagcc
      301 gtctgccaag aacagccatc atgtctgcct tttctgagtc aatgtcgtaa agcacaatct
      361 cggagcaact cagttcaggg gtcaacgcga agtctcccaa cgccctcgga aaatacagac
      421 ttccggcacc aaggcaggcg attttcttct gaaccatctt tgttgccctc attcttcatc
      481 agatgttttt ggcatcatag tgaatcatgt cagcctcgac aatcaatttt ccattcccca
      541 tttgcgttga agtcctgtag tatccctaaa gtcacaacgc aacgggattc acgacggacg
      601 tgagttcatg agcatatata tgaggtgtac gtaccaatgg ccaagaagcc gatcacagca
      661 atcatcgtag gtgccggcca ccgatcgctc agttacgctg gatatgcgaa gcatcatccc
      721 gatgagctcc agatcgttgg agtcgccgat ctgatcccgt tgcggagaga actggttgcc
      781 cgactctacg gtctctcccc ggagcagtgc ttcgcaactg cagaagaact cgcccaacgt
      841 ccccgaatgg ctgacgcgat catcaatgga accatggatc atcagcacgt cgccacatcg
      901 gtgcctcttc ttgaggcggg ctacgatatg cttctggaga agcccttcgc caccaacgag
      961 gaggaaatgt ggcaacttgt caacacctgt cgaaaacacg ggaacaaggt catgatttgc
     1021 catgttcttc gctatgcccc tttctacgcc gcaataaggc agaaagtgat tgatggtgag
     1081 attggcgaaa taatcaactg tcagttcgct gagcacgtca gctaccacca catggctgtg
     1141 ggctacgttc gaggcaaatg gaacagaaaa gaccgatgtg gttcgtcaat gctcatcgcc
     1201 aaaagctgcc atgacctgga cttgatgaca tggatgaaat ccggcatcga accagtccga
     1261 gtctccagtt ttggttccaa catgcagttc c
//
LOCUS       DAOQUC010001362         1290 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_149583_length_1290_cov_3.106883, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001362 DAOQUC010000000
VERSION     DAOQUC010001362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1290)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1290)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1290
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_149583_length_1290_cov_3.106883"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..852
                     /locus_tag="PKH07_18350"
     CDS             <1..852
                     /locus_tag="PKH07_18350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36957.1"
                     /translation="WVQLGTDAYYFHQGFSGSVVMTNEDIDLSGNMFAGGVKFEYQTP
                     EAPDKNYTVNYLGTSDAKPVINGLVSAGEWDAAEAAGTGYVLHNNPAVPAVEDGSFRM
                     LFDDSHLYILFEMNNSYLAGYTTPPPSYGYFDLLGDRINFFLSPFGTSTEPFYRILFS
                     PNPSDGNCYVWSQASLIKTTDANVGRDWVAGGDIAYTHVSGQLTVEYRVPWSEFDYPG
                     ISVAMRPESGTVWGVQPCITNEISAGVWESVNWEPDDTPSYVYGVPFGGLQFNGGASS
                     LSGWELY"
     gene            complement(941..>1290)
                     /locus_tag="PKH07_18355"
     CDS             complement(941..>1290)
                     /locus_tag="PKH07_18355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36958.1"
                     /translation="AYLTSLPLDIFNRRQRYKYIRPGKGWANGVPTTLAIWVPRLFPN
                     DGGPEQDVPYFQPEPSPVKWALWSVGPPGPLTFYDSSMRNIPVPRRNWYDPTNGTCSQ
                     GILPRLSTGHTAP"
BASE COUNT          285 a          329 c          371 g          305 t
ORIGIN      
        1 tgggtgcagc tcggtacgga tgcctattac tttcaccagg gcttcagtgg ttctgtggtc
       61 atgacaaatg aagacattga cctaagcggc aacatgtttg cgggtggcgt caagttcgaa
      121 tatcagactc ccgaggcacc cgacaagaac tacaccgtca attatctggg aacttcggat
      181 gccaagcctg tcatcaacgg tctggtgagc gcgggagaat gggatgctgc cgaggcggca
      241 gggaccggtt atgtgcttca caacaatccc gctgtgccgg cagtggaaga cggctctttc
      301 cgaatgctct ttgatgactc ccacctgtac atcctctttg agatgaacaa cagctatctt
      361 gccggctaca ccacacctcc cccgtcctat gggtatttcg atcttttggg agatcgaatc
      421 aacttctttc tttctccctt tggcacgagc actgagccat tctacagaat cctcttctct
      481 cccaatccgt ccgatgggaa ctgctacgtt tggtcgcagg caagtctgat caagacaacg
      541 gatgccaacg ttggcagaga ttgggtggca ggaggagaca tcgcatacac ccacgtgagc
      601 ggacagttga cagttgaata cagggtcccc tggagcgaat tcgactaccc cggaatcagt
      661 gttgcgatgc gacccgaaag cggtaccgta tggggtgttc aaccgtgcat caccaatgag
      721 atcagcgcag gagtctggga atcggtcaac tgggaaccgg acgacacacc ttcgtatgtt
      781 tacggtgttc ccttcggcgg ccttcagttc aatggcgggg catcgagcct gagtggctgg
      841 gaactgtact gagatgtaac catctccgct gaatcagatg ggaaagtgaa ccgtttgcaa
      901 cagagaacgt ctgttgggtg tagtcgttgt tcgttcacct ctaaggagcc gtgtgacctg
      961 tgctcaggcg cggaagaatt ccttgtgaac acgtgccgtt tgtggggtcg taccagttgc
     1021 gcctgggaac gggaatgttg cgcatgctgc tgtcatagaa cgtaagtggc cccggcgggc
     1081 caacgctcca gagtgcccat tttacaggcg atggttcagg ctggaagtaa ggcacatcct
     1141 gttccggacc gccatcattt gggaagagcc ggggaaccca gattgctagt gtggtaggca
     1201 cgccgtttgc ccagcctttc cctggacgga tgtatttgta gcgttgcctg cgattgaaga
     1261 tatccaacgg caatgaggtt agataggcta
//
LOCUS       DAOQUC010001363         1288 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_149943_length_1288_cov_3.244120, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001363 DAOQUC010000000
VERSION     DAOQUC010001363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1288
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_149943_length_1288_cov_3.244120"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1288)
                     /locus_tag="PKH07_18360"
     CDS             complement(<1..>1288)
                     /locus_tag="PKH07_18360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36959.1"
                     /translation="GDLLLSWAQQQTVSKTRLENEAADCYRKALDTDPGNVMASVGLA
                     LFHLLRNTPDEALRLLEAVRKEYQDAHPDLPLPVALSYALNSAYKSKGFTLKQERSLL
                     QTAEGSGLASRLALVDLIDLWISQRAHSRAFEFAKTKLERGISLRDGSAYTALLRAFP
                     KESPERERILRTVLDFRPSDRSLFMNLGDSLASRKDWEDALKAYHYAAQLCPPDPKPW
                     EAMASLHQVRLASGETVTEASTEVVRCLTESLKRDQNPQIRDRLFCFQTANGHQPQKP
                     FHLLQRYDVSLDDVLKEQIPWHKYGLGSSALIVDTAVARLHRNGTREMLTHQSARILN
                     QQGIDAWAELTLPADESSTWVLAAHTRTEDGLQMLPGNVTRGTGARAVSLSGVQVGSV
                     VDFCYVETVSSPGTPGANFWTEEFFFGQKEDATYLCR"
BASE COUNT          291 a          354 c          360 g          283 t
ORIGIN      
        1 cggcagagat aggttgcgtc ttccttctga ccaaagaaga attcctctgt ccagaaattc
       61 gcgccgggcg ttcctggact tgataccgtt tccacgtaac agaaatcaac gacagaacca
      121 acttgaaccc cggaaagtga aacggcacgc gctcccgttc cgcgagtgac gtttcccgga
      181 agcatctgca ggccgtcctc agtacgggtg tgcgctgcca gaacccatgt ggatgactcg
      241 tctgccggta atgtcagttc cgcccacgca tcaatgcctt gttgattgag aattctggcg
      301 gactgatgcg tcagcatctc ccgtgtgcca tttcgatgca gcctggcaac agcggtatcc
      361 acgataagcg cacttgagcc cagaccgtat ttgtgccaag gaatttgttc cttaagaaca
      421 tcgtccaagg agacatcata gcgctgcaga agatggaaag gcttttgagg ttggtgtccg
      481 ttggcagtct gaaaacagaa gaggcgatcc ctgatttggg gattctgatc ccgcttcagc
      541 gactcggtca gacagcgcac cacctcagtc gaggcttccg ttaccgtttc gcccgaggca
      601 agccgcactt ggtgcagtga agccattgct tcccagggtt tcggatccgg tggacacagc
      661 tgggctgcat aatgataggc tttgagcgca tcctcccagt ctttgcgcga agccagactg
      721 tcgcccagat tcataaagag gctgcggtca ctgggacgaa aatcaagcac tgttctcaga
      781 atccgttccc gttctggaga ctcttttgga aaagcccgaa ggagtgccgt gtaggcggaa
      841 ccatcccgaa gggaaatccc ccgctccaac ttggtcttcg caaactcaaa ggctctggaa
      901 tgtgcgcgct gcgagatcca gagatcgatc agatcaacca gtgccaaccg ggaagcaagc
      961 cctgagccct cagcagtctg aagcagagac ctctcctgct tgagggtaaa gcccttgctc
     1021 ttgtaggctg agttgagggc gtaactcaga gcgacaggca gaggcaaatc gggatgtgca
     1081 tcctgatatt ctttgcgaac cgcttcgaga agtcgaaggg cttcgtcggg agtgtttcgg
     1141 agcagatgaa aaagcgccag accgacgctt gccatcacgt tgccgggatc cgtgtcgagt
     1201 gccttgcgat agcagtctgc tgcttcgttc tccagccgcg ttttggagac agtctgttgc
     1261 tgtgcccagg acagcaacag atcgcccg
//
LOCUS       DAOQUC010001364         1288 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_149952_length_1288_cov_3.040552, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001364 DAOQUC010000000
VERSION     DAOQUC010001364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1288
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_149952_length_1288_cov_3.040552"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..658)
                     /locus_tag="PKH07_18365"
     CDS             complement(<1..658)
                     /locus_tag="PKH07_18365"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNT36960.1"
                     /translation="MSKKFRVGFIGCGGIAGRHFNDLAEEKRAKVVAMCDPDPNRIKT
                     WKERRPELLKAAEYADYKTMLKKEELDVVYINSPHNVHYEQIMNSMKAGCNVIAEKPM
                     VCSIEHARKVCAASKRLGKAVIVAYQRRYQSGYRKVREIVDSGELGRLLAVTAYQLQG
                     WLNGTKGTWRQCKEMSCGGQLNDSGSHMIDILLFMINDVPKEVFCFLDNRGTEVDIDS
                     A"
BASE COUNT          288 a          342 c          335 g          323 t
ORIGIN      
        1 ccgcggagtc aatgtccact tcggtgcctc ggttgtcgag aaaacagaac acttccttgg
       61 gcacatcgtt gatcatgaaa agcaggatat caatcatgtg gcttccggaa tcgttcaact
      121 ggccgccaca ggacatctct ttgcattgtc tccaggttcc tttggtgcca ttgagccatc
      181 cctgcagctg ataggccgtc acggcaagca gacgtcccag ttctccactg tccacgatct
      241 cacgcacctt gcgatacccc gactggtaac gacgctgata ggccacgatg accgctttgc
      301 cgagacgctt gctggctgcg cagactttgc gcgcatgttc gatgctgcat accatcggct
      361 tttcggcaat gacgttgcag cccgccttca tgctgttcat gatctgttcg tagtgaacgt
      421 tgtgcgggga gttgatatag acgacatcca actcctcctt cttgagcatg gtcttgtagt
      481 cggcgtactc ggctgccttg agcagctcag gcctacgctc cttccaggtc ttgatccggt
      541 tcggatccgg atcgcacatc gcaacaacct tggctctctt ctcttcagcg aggtcattga
      601 aatgtcttcc cgcaattcca ccacatccga tgaacccgac tcgaaacttc ttgctcatag
      661 cgccctgttc ctcctgtcag tgcctttgtc tggatgattt ctcaactggg tggcaactga
      721 cgtaaattga aaagaaatct agcactccgg caggacttgt ccacaggaaa aaataaacca
      781 gtctccagtt tgcgatacac tctctgaaag ccggtgcgac tcaaccatgg aatacgcaga
      841 aggatgattc atcgctccgg attcagttgg atgaagcaaa gatcggcttg tccgaaagaa
      901 agggagagcc ccaaaccgtt tgaactcctc cggaatactt ggtacgctct gattcaatag
      961 ggcacctttc gaggattctt tgaaagatga tgggaagggg tatcgtatta ttgatcgatg
     1021 gtgttggcat ctctgccagc ccagatgaag ggatgcactg ggtgcaaagc gctttgtcat
     1081 ttcggacgag gtgacgctct gggagcgaag cccttgtcat tccgagtgca gcgacgctcg
     1141 gggagcgaag agaggaatct ctctgcaacc caacggagag atttctcgca gagcctcgaa
     1201 tgacaagaag cgggtttgtg gtgacactgt gaggcgtttt tgggacacgc ctttatggat
     1261 acactacggt cttatcctca ggagggtt
//
LOCUS       DAOQUC010001365         1286 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_150283_length_1286_cov_5.102356, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001365 DAOQUC010000000
VERSION     DAOQUC010001365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1286
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_150283_length_1286_cov_5.102356"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(422..>1286)
                     /locus_tag="PKH07_18370"
     CDS             complement(422..>1286)
                     /locus_tag="PKH07_18370"
                     /inference="COORDINATES: protein motif:HMM:NF023149.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="malectin domain-containing carbohydrate-binding
                     protein"
                     /protein_id="HNT36961.1"
                     /translation="IFSLLTGEPLNMTSPITGESLRWTWKRDYGCNYPIACENFVSFR
                     SAAAGYYDLENQSGTGHFGGFKSGCTSNLIAADGVLNAPDYTRTCSCSYQNQTSLAMV
                     HMPDNEAWTFTTLERGKEPIQRLGINLGAPGDRVSETGTVWLEYPIEGGPSPKIIVSV
                     SPEKVIYHRVHSSRVAEETMNWVFASGVTGLDRLSVQVGNKTPSAYSVRLFFCETRAK
                     STQNNSFDVALQGLTVLRDFDVLKESGGLNQGIVKEFAGVAIGENLEVTFASDDQKST
                     LLSGIEIVAEQ"
BASE COUNT          317 a          342 c          328 g          299 t
ORIGIN      
        1 atgcaacgta cgtcaccaag tcccagttgt tggaacatgc ggctctgcac cagaggcatc
       61 tgtgcccagt tgatcgccgc agagttcaac agcaagtaga taaacaacaa ccccgccaaa
      121 agctttctca ccgttgtgac ctcctcaaac cctgatccca gtctggaagc gactcacatc
      181 tgaactgcac cgggctgtcc atcggcgtcg tcatagtctc ttcgtagaca agacggcgga
      241 tgtcgaagaa tggtatggac atccttcatc tgcatatacg agaagatctc aagagatatt
      301 tccaagcgtc ttgggaaact tcctgatatt cagtaattta ggaagggatc agagaagatg
      361 caagcacatt ctgctatgta cgggcaacaa agcgacgacg cccttaatga cgttcgtctg
      421 ttcactgttc cgcaacgatc tcgatgcctg aaaggagtgt gctcttttga tcatcggatg
      481 caaaagtaac ctcgaggttt tctccgatgg caacaccggc gaattccttg acgatgcctt
      541 gattcaagcc acctgactct ttcaggacgt cgaaatccct cagcacggtc agcccttgga
      601 gtgcaacatc aaaggaattg ttctgggtgg attttgcccg ggtttcacag aagaacaggc
      661 gaactgagta agcacttggc gtcttgttcc caacctgtac actcagccgg tcaagtccag
      721 taacgccaga cgcaaatacc cagttcatcg tctcctcagc aactcgcgac gaatgaactc
      781 ggtgatagat cactttttca ggactgacgg aaacgatgat cttcggtgat ggaccgccct
      841 cgatgggata ctccagccag acggttcctg tctccgaaac tcggtctccg ggtgcgccca
      901 ggttgatgcc cagccgttgg ataggttcct tgccacgctc cagtgtggtg aatgtccatg
      961 cttcgttgtc cggcatgtga accatggcaa gcgacgtctg gttctgatac gagcaggagc
     1021 aggttcgtgt gtagtcggga gcattcaaca ctccatcggc agcaatgaga ttagaggtgc
     1081 agccggactt gaagcctccg aaatgtcctg tgccggactg attctccaag tcatagtaac
     1141 ctgcggcagc ggatcgaaat gaaacgaagt tctcacacgc aatggggtag ttgcagccgt
     1201 agtccctttt ccatgtccag cgcaacgatt cacccgtgat tggagaggtc atgttcagag
     1261 gctccccagt cagcagggag aaaatg
//
LOCUS       DAOQUC010001366         1284 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_150723_length_1284_cov_3.483320, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001366 DAOQUC010000000
VERSION     DAOQUC010001366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1284
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_150723_length_1284_cov_3.483320"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..308)
                     /locus_tag="PKH07_18375"
     CDS             complement(<1..308)
                     /locus_tag="PKH07_18375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36962.1"
                     /translation="MVIFAGVMILSILSVFATPVARANEAAKGSEVLGDGFFHHGVAT
                     PISNHRGTVATVDGDGRNVALVWLFDHRGGYALLMVDAETGRSEEFPMPFPPGGDCPY
                     A"
     gene            complement(509..>1284)
                     /locus_tag="PKH07_18380"
     CDS             complement(509..>1284)
                     /locus_tag="PKH07_18380"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNT36963.1"
                     /translation="FPKPLAYDASQYEILARLFESGADPRIRWSRDTNNHHLFDGAYF
                     IDYVSGADEWPGGDWLTRERIFQEHVNYQLGVMWFLQNSERIPEEYQTIFKKWGLPRD
                     IYLDTGGWSHQLYIREGRRMVSDYVMTEHNCMRREIPEDSIGLATYNMDSHHCQMTVI
                     DGAVQNEGNIEIRPKGPYPISFRAITPKSAECTNLLAPLSLSASHIAFGSIRMEPVFM
                     ILGQSAGVASCIAIDDQVRIQDVEYSKLREKLLAAGQKL"
BASE COUNT          296 a          368 c          318 g          302 t
ORIGIN      
        1 gcgtaagggc agtctccacc cggcgggaag ggcatcggga attcctcact cctgcctgtc
       61 tcggcatcca ccatcagcag ggcatagcca cctcggtggt cgaacaacca gacaagtgcc
      121 acgttccttc cgtctccatc aaccgtggca acggtgcctc ggtgattgct gataggagtt
      181 gccactccgt gatgaaagaa accatctccg agtacttcgc tccctttggc ggcttcattc
      241 gcccgagcca ccggtgtggc gaaaacagaa agaatactca agatcattac ccctgcaaaa
      301 atcaccatag acttaggtcc tttcgcctca tgattcttca atcgctttct ccttgaatgc
      361 agatcggagc ctggttcaaa cccgatgaga cagttctctg tttaccaggt cacccgatat
      421 tctcactcat tctctttccg tcagcgcttt gacacctgct ctttgaagcc aggtcacaac
      481 caaagacttg cccgccatgg aacgtctctc acaacttctg tccagccgca agcagtttct
      541 ctctcaactt agaatactcg acatcctgga tcctcacctg atcatcaatg gcgatgcagg
      601 aagcgactcc tgcggactgc cccagaatca tgaacaccgg ttccatccga atcgaaccaa
      661 aggcgatatg cgaagcggaa agactcaaag gagccagcag attggtgcat tcagcggatt
      721 tcggcgtgat cgctcggaag gagatcggat acggcccttt cggtctgatt tcgatgttgc
      781 cctcgttttg aacagcgccg tctatgacgg tcatctggca atggtgggaa tccatgttgt
      841 aggttgccaa accgattgag tcttcgggaa tctcacgcct catgcaattg tgttcagtca
      901 tgacatagtc gctgaccatt cgacgacctt cgcggatgta gagctgatgc gaccagcccc
      961 cagtgtctag gtagatgtca cgaggcagcc cccacttctt gaaaatcgtt tgatattcct
     1021 caggaatccg ctcgctgttc tgcagaaacc acatgacacc gagttgatag ttcacatgtt
     1081 cctggaaaat gcgctcgcga gtcagccagt cgccgcctgg ccattcgtcc gcgccgctta
     1141 catagtcaat gaaataggcg ccatcgaaaa ggtggtggtt gttcgtgtct ctgctccagc
     1201 gaatcctggg gtcggcgccg gattcgaaca agcgcgccag aatctcgtat tgggaagcgt
     1261 cataggcgag aggcttggga aaag
//
LOCUS       DAOQUC010001367         1283 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_150949_length_1283_cov_3.302117, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001367 DAOQUC010000000
VERSION     DAOQUC010001367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1283)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1283)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1283
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_150949_length_1283_cov_3.302117"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            14..>1283
                     /locus_tag="PKH07_18385"
     CDS             14..>1283
                     /locus_tag="PKH07_18385"
                     /inference="COORDINATES: protein motif:HMM:NF025229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HNT36964.1"
                     /translation="MTPKTDFSLSCNVWSASAEVDGSLTVRVRTLTDSSPLQVTKAFV
                     AREQPQRISVIGSAPGAERCKVYTIPVLSAGNYGVEVKLGTLPDSVVEWYADGQLVAL
                     DDDGGEGLGSRLVMHLEPGVYFALVRGWQIGQYGTFTIQFGGVETTQESTPLFPSSEL
                     SPSTANSYPYADNGSEPYTISGSVRGTNGLPVAGVQVIVPETTLGAETDERGRFLIRN
                     IPAGFSGQVAVEQTDGLVLPATRSFSAPTCLLDFDLLRSVGTLQSGERLEGSIEDFRS
                     LAEGFVVSATENSWIGSSDYALLVAEMDFRDFSPNEDGVRDFCTLTYQLSEDATTEVI
                     IRDLDGHLVRQLRSPTLANMGSHRVMWDGTDYLGESLPEDTYLIQIVVRDKESVQHSL
                     DFCVSINRSFFFHPANSADLANRLLLGAWFR"
BASE COUNT          277 a          321 c          366 g          319 t
ORIGIN      
        1 tcagcagtgg gcaatgactc ccaagacgga tttttcgctg tcgtgcaatg tctggtctgc
       61 atcggcagag gtggatggtt cgttgactgt tcgggtgcgc actctgacag acagttcgcc
      121 tcttcaagtg acgaaagcat tcgtcgctcg cgagcagcca caacgcatat ctgtcatcgg
      181 aagcgcccct ggtgcagaga gatgcaaagt ctacacaatt cctgtactga gcgctgggaa
      241 ctatggtgtc gaagtcaaac tcggaacgct tcccgacagc gttgtggaat ggtacgccga
      301 tgggcaattg gtcgctctcg atgatgacgg tggtgaaggt ctgggttcca gacttgtcat
      361 gcacttggaa cctggagtct atttcgcctt ggtgcgagga tggcagatcg gacagtacgg
      421 gacgttcaca attcagtttg gtggagtgga gacgacacag gaaagcactc cactgtttcc
      481 gtcctcagaa ttgtccccaa gcaccgcaaa cagttatcca tacgcggaca atgggtctga
      541 gccctatact atctccggat cagtccgtgg aacgaacggt cttccagtcg caggagttca
      601 ggtcattgtg cccgagacca cactcggagc agagactgat gagcgagggc gattcctcat
      661 tcgcaacatc cctgcaggtt tctctggaca ggttgctgtt gaacagacag acggcttggt
      721 gttaccagcc acgcggagtt tttcagctcc gacgtgcctt ctcgacttcg atctgctgcg
      781 ttccgttgga accttgcagt ctggagagcg acttgaaggc agtatcgagg atttccgttc
      841 gcttgcagaa ggttttgtcg tgagcgcgac ggagaattca tggattggat cgagcgacta
      901 cgcgcttctg gtggcggaga tggactttcg tgacttctca cccaatgaag atggcgttcg
      961 cgacttctgt acattgacct atcaactcag tgaagacgcc acgacagagg ttattatccg
     1021 cgatcttgat ggacacttgg tgcggcagct gagatcgcca acgctggcca acatgggatc
     1081 ccatcgcgtc atgtgggatg ggacagatta tctgggggag tccctgccgg aagacactta
     1141 tctgatacag attgtcgttc gtgataaaga gagcgtacag cacagtctgg acttttgtgt
     1201 ttccatcaac cgttcattct tctttcatcc agcgaacagc gccgatttgg caaatcggct
     1261 cctgttaggt gcctggtttc gct
//
LOCUS       DAOQUC010001368         1282 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_151133_length_1282_cov_3.129584, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001368 DAOQUC010000000
VERSION     DAOQUC010001368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1282
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_151133_length_1282_cov_3.129584"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1282)
                     /locus_tag="PKH07_18390"
     CDS             complement(<1..>1282)
                     /locus_tag="PKH07_18390"
                     /inference="COORDINATES: protein motif:HMM:NF027452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HNT36965.1"
                     /translation="LSNPDVLSIATKTVLKWIVEHPEVPIIDVSQNDGTGACECDSCV
                     AIVNEEGSQHGPILRFVNAIADEVAKKYPDKWIETLAYAYSTAPPRITKPRNNVIIRL
                     CHAGCFFHGFERCDLGSQHALWLEGWSKLTNRIFVWYYGTNFRHYLGPNPNLDGLARD
                     IKYYGDHRVNGVMVQCNYQGHGGELAELRQYLCAQLLWDPTQDPMKIREDFCRGYYGE
                     AWAVVMEFLAKMDEFARGDAHVFAVWEPQDIMSPEFVAEALTLLETAYSLSDTPAIRN
                     RIEKLMLPYWYLYLRYPTKYGLTKEEAGAYLKPVKRVIEANAIEFVCEGGEPNAQQWL
                     AQIEAEYGVLEEGVLYSLLSEKPVETVNCDDWRLNSVEREGRHVATILHHPPAEGDGT
                     ATFELRLPPNEKTALTFATVIAAPTVDGVRFSILV"
BASE COUNT          293 a          370 c          315 g          304 t
ORIGIN      
        1 acgaggatcg agaatcggac accatccact gtgggagccg caatgacagt agcaaaggtc
       61 aaagccgttt tctcgttcgg cggaagtctc aactcgaatg tggcggtgcc gtcgccctcg
      121 gctggcggat gatgaagaat cgtcgcgaca tgcctaccct ctctttcgac gctgttgagc
      181 ctccagtcgt cgcagttgac tgtctccacc ggcttctcgc tcaaaagact atacaagacg
      241 ccttcctcca ggacaccgta ctctgcctct atctgagcca gccattgctg agcgttcggt
      301 tcgcctccct cacaaacaaa ctcgatcgcg tttgcctcaa tgacccgctt gacgggcttc
      361 aggtaagcgc ctgcttcctc tttcgtcaag ccgtactttg ttggataacg cagataaaga
      421 taccaatagg gaagcatcaa tttctcgatc cgattccgga tcgccggagt gtcggaaagg
      481 gaataggcgg tctccaagag ggtcagcgct tcagcgacaa actcaggcga catgatatcc
      541 tgtggctccc agaccgcgaa aacatgggca tcgcccctgg cgaactcatc catttttgcg
      601 agaaactcca ttacgaccgc ccaggcttcc ccgtagtagc ctcgacagaa gtcttccctg
      661 atcttcatcg gatcctgtgt cgggtcccag agaagttgag cgcaaaggta ttgtcgcaat
      721 tccgcaagtt cacccccatg tccctgataa ttgcactgga ccatcactcc gttgactctg
      781 tgatccccat agtacttgat gtccctcgcc aaaccgtcaa gattcgggtt cggtccaaga
      841 tagtgccgaa aattcgttcc gtaataccaa acaaagatgc ggttggtgag tttgctccat
      901 ccttcgagcc atagtgcatg ttgcgaacca agatcgcacc gctcaaaccc gtggaagaaa
      961 caacctgcgt gacatagccg aatgataacg ttgttgcgtg gtttcgtgat tctcggcggg
     1021 gcagtggaat aggcataggc tagggtctcg atccatttgt cggggtactt cttcgccact
     1081 tcatccgcga tggcattcac gaaccgcaaa atcggtccat gctgtgagcc ttcctcgttg
     1141 acgatagcga cacaactgtc gcattcgcaa gccccagtcc cgtcgttctg tgacacatct
     1201 atgataggga cttcaggatg ctctacgatc cacttcagaa cggtctttgt ggcaatactc
     1261 aggacatcag gattgctcaa gc
//
LOCUS       DAOQUC010001369         1281 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_151318_length_1281_cov_3.601142, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001369 DAOQUC010000000
VERSION     DAOQUC010001369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1281
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_151318_length_1281_cov_3.601142"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1038
                     /locus_tag="PKH07_18395"
     CDS             <1..1038
                     /locus_tag="PKH07_18395"
                     /inference="COORDINATES: protein motif:HMM:NF018877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HNT36966.1"
                     /translation="VVSNDVYLWSQLRAIWTFSALFNRIEPRQEWLDIARDLFDFCRR
                     HGRDSEGRWCYRLSPQGEILDGPISIYADGFAIYGLCEYYKATQDERAWEIAFQTFRN
                     VQKRLAVPGSYQTAPYIIPQGAKAHAVAMIFSYFFHEMGKVFDSPEILQAGLTHAREV
                     MDHFLRPESGLVLEYVTLDGKTLPGAEGRIVVPGHAIESMWFQMQIFRDHDEDDMIRK
                     AVNAVQKHLEFGWDAEHGGLFLGGDSEGQAPVWPNWNKKIWWPHTEALYALIRAREYS
                     HEPWIDEWFSRIFDYCFTHFYDRKHGEWTQRLDRFGNKITEVVALPVKDPFHLPRALI
                     LSIESLERQMH"
     gene            1078..>1281
                     /locus_tag="PKH07_18400"
     CDS             1078..>1281
                     /locus_tag="PKH07_18400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36967.1"
                     /translation="MELEFKPDFERARKMWTSFWEESNLRPLVAIVRPKDGVEPAPAP
                     ATYEMTRGDLNLVLDKIVQWASTH"
BASE COUNT          304 a          340 c          345 g          292 t
ORIGIN      
        1 gttgtctcca atgatgtcta cctctggtcg caactgcgtg ccatctggac attctcggca
       61 ctcttcaacc gaattgagcc tcgacaggaa tggctcgaca tcgctcgaga cctgtttgat
      121 ttctgtcgcc gacatggcag ggattccgaa ggtcgttggt gctatcgcct cagcccacaa
      181 ggggagattc tcgatggccc catcagcatc tacgccgacg gatttgccat ctacggactg
      241 tgcgaatact acaaagccac ccaggatgag agagcctggg agatcgcctt ccagacattc
      301 aggaatgttc aaaagaggct tgcagtgcct ggaagctatc agacagcgcc ttacatcatc
      361 ccgcaaggcg caaaggctca cgccgtagcg atgatcttct cgtacttctt ccacgaaatg
      421 ggaaaagtct ttgactcccc agagattctt caagcaggtt tgacccacgc cagagaagtc
      481 atggatcatt ttcttcgccc tgaatccggt ctcgttttgg agtacgtcac gctcgacgga
      541 aagacgcttc ccggtgctga agggcgcatc gttgtgccgg ggcacgccat cgagtccatg
      601 tggtttcaga tgcagatatt tcgcgatcac gatgaggatg acatgatccg aaaggctgtc
      661 aacgccgttc agaagcatct ggaattcggt tgggatgccg aacacggtgg ccttttcctt
      721 gggggagata gtgagggaca ggcacccgta tggcctaact ggaacaagaa gatctggtgg
      781 ccgcatacag aggcactcta tgctttgatc cgtgctcggg aatactctca tgaaccgtgg
      841 attgatgaat ggttcagccg aatctttgac tactgcttca cgcacttcta cgacaggaaa
      901 cacggagaat ggacacaacg ccttgaccgg tttggaaaca agatcacgga ggtggtggct
      961 cttccggtca aagatccatt tcatctccca cgggcgctca ttctgtccat cgaatctctg
     1021 gaacggcaaa tgcattgagt cgtaaagcca cagaagaccg catggagtga ccacgctatg
     1081 gaactggagt tcaaacccga ttttgaacgt gcccgaaaga tgtggacttc gttttgggaa
     1141 gaaagcaatt tgcggccgct cgtcgccatc gtgcgtccta aagacggagt ggagcctgcc
     1201 cccgctcccg ccacctacga gatgacgcga ggagatttga acctggtgct cgacaagata
     1261 gttcagtggg cttcaactca c
//
LOCUS       DAOQUC010001370         1280 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_151673_length_1280_cov_1.706939, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001370 DAOQUC010000000
VERSION     DAOQUC010001370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1280
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_151673_length_1280_cov_1.706939"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1280)
                     /locus_tag="PKH07_18405"
     CDS             complement(<1..>1280)
                     /locus_tag="PKH07_18405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36968.1"
                     /translation="PLSTIGDLITEASVDLLGNDVADMAFMITNLAEQTLVARVPFEW
                     SGSIPTQQSTKVVEVYHPEFTTAPDGTTVPDALVPQNEACALTIPPRETRQIWIRFRS
                     QDIKSGSYHGSLSVVPLVLSQEVSTVEITLNVLPVSLPQSQRERVFVWDYQGDAERLG
                     LEPQYHQMLADHGVNVFIIHALHDLPRNAIADDGKFMEPLDFSRFRRMVSLKNPGMLY
                     LSMDIWEKQKVRSAFGQEYYNILWQQAFKEIMKQTLKELKSLKIEPTDVVVNPVDESC
                     DQRYIEIARLWREIDPNVKLIADASTKDASEFQAVDPYVDIWCPHMKTLGLPEYAEFY
                     NWVRATGKPVWAYWYSEGANEKARAPLEYRMRHWTAFGHDLQGLGYWTATQHYGDPWH
                     RYLSQAAYDPSLIYPGKTQAIPSRRWFAFHRGVL"
BASE COUNT          316 a          353 c          329 g          282 t
ORIGIN      
        1 tccagaacac cccgatgaaa agcaaaccat cttctggacg gtatggcctg tgtcttacct
       61 ggatagatca gcgaagggtc ataggccgct tggctcagat accgatgcca tggatctcca
      121 tagtgctgag tcgccgtcca gtagccaagt ccctgcaagt catgcccaaa agcagtccag
      181 tgtctcatgc gatactccag gggcgctcgc gccttctcgt tggcaccttc ggagtaccag
      241 tacgcccaga ccggtttgcc agtggctcga acccagttgt aaaactccgc atactcgggc
      301 agaccaagcg tcttcatatg aggacaccag atgtccacat agggatcaac agcctgaaac
      361 tccgaagcgt ccttggtgga agcgtctgcg atcaacttga cgtttggatc tatttcacgc
      421 cagagcctcg caatctcgat atagcgctgg tcacaggatt catcaaccgg attgacaacg
      481 acatccgtcg gctcgatctt gagcgacttc agctctttga gcgtctgttt catgatctcc
      541 ttgaatgcct gttgccagag aatgttgtag tactcttgtc caaaagccga acgaactttt
      601 tgcttttccc agatgtccat cgaaagatag agcattccag gattcttgag agagaccatc
      661 cggcggaaac gagaaaaatc caagggctcc ataaacttcc catcatccgc aatggcattg
      721 cgcggaagat catgcagcgc gtgaatgatg aagacgttga ctccatgatc agcaagcatt
      781 tgatggtact gcggctcaag tcccaatcgc tcggcatcac cctgatagtc ccacacgaag
      841 actctctctc gctgcgactg aggcaaagaa acgggaagaa cgttcaacgt gatctcgacc
      901 gtggagactt cctgggacaa gacgagtgga acaacgctca ggctcccatg gtaactgccg
      961 gacttgatgt cctgagaacg aaaacgaatc cagatctgcc gggtctctcg cggtggaatg
     1021 gtcagcgcac aggcctcatt ctgcggaacg agggcatcgg gaactgtcgt cccatcgggc
     1081 gcagtagtga actcgggatg gtagacttcc actaccttgg tgctctgttg cgtcggaatg
     1141 ctgcctgacc actcaaaagg aacccttgcc acgagtgtct gctccgccag attggtgatc
     1201 ataaacgcca tgtcggcaac atcattgcca agaagatcaa ccgaggcttc agtgatcaga
     1261 tcgccaatcg tgctgagggg
//
LOCUS       DAOQUC010001371         1279 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_151738_length_1279_cov_3.246732, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001371 DAOQUC010000000
VERSION     DAOQUC010001371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1279
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_151738_length_1279_cov_3.246732"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1279
                     /locus_tag="PKH07_18410"
     CDS             <1..>1279
                     /locus_tag="PKH07_18410"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNT36969.1"
                     /translation="AMVGLVSGRVTDALTGIPIPGAVVTISGGHHASTDSRGFYRIID
                     VPAGEAKVTYAESEGYLPVRTANVSVSVGATASVDFALTPSSFTALPPNVDKPQGDPT
                     EVFVSYTGGDLDKLVSVGRHSVFVNRDYALPLVGQNAAGRLRNPSAAVQQGILPESVV
                     VELSVLGLHHLDEIIDLHVNGFRIPRSLIQVVPSRYRWQRVRARFPTSANWLMFSREG
                     AIPNTLPNGAENQIYLELRRPALVDWASISLSVAGLPNTVGTITGAYQESVADANANW
                     QFDHLLIQLGLETQLRGECFAISRLESVSGSGVADAQVWLDLEAGDNLLPLYFPGHQI
                     HASAVNGPYRIHWLALYTADASATYQLDYTTKIYDFQDFEGVGLSSEATPTPPPTRKP
                     TPTSYPDFTGDYLVNSLDLFLFGRFWRQPSPRAP"
BASE COUNT          257 a          354 c          341 g          327 t
ORIGIN      
        1 gcaatggtgg gtctggtctc cggccgagtg actgatgcgt tgaccggcat acccatccca
       61 ggcgctgtgg tgaccatttc aggcgggcat cacgcctcaa ccgattctcg aggattctac
      121 cgcatcattg acgttcctgc cggagaggca aaagtgacct atgcggaatc ggaaggctat
      181 cttcctgttc gaacggcaaa cgtctccgtc tcagttggag cgaccgcttc cgttgatttt
      241 gcgctcacac cgtcatcttt cacagcgctt ccgccgaatg tggacaagcc tcaaggagat
      301 ccaactgaag tgtttgtttc atataccggt ggcgatctgg acaagttggt ctccgtcggg
      361 cgtcattcgg tttttgtcaa cagggactat gcattgcccc tcgtcggcca gaatgcggcg
      421 ggtcggcttc ggaatccttc tgcggcagtt caacaaggca ttctgcctga atctgtggtt
      481 gttgaactgt cggtgctcgg acttcatcat cttgatgaga tcattgatct tcatgtcaat
      541 ggatttcgca ttccgagaag cctgattcag gtggtgccgt cacgatacag atggcagcgt
      601 gttcgtgcca gatttccaac gtctgccaac tggctcatgt tttcgagaga aggtgccatt
      661 cccaacacgc tgcccaatgg ggcggagaat cagatctact tggagttgcg aaggcccgcc
      721 ttggttgact gggcaagtat ctcgctttct gttgcagggc ttccaaacac agtgggtaca
      781 ataacaggcg cctatcagga gtctgttgcc gatgcaaatg ccaactggca attcgatcac
      841 cttctcatcc aactcggctt ggagacgcag ctgcggggag aatgctttgc catctcacgg
      901 cttgaaagcg tttctggcag cggtgttgct gacgcgcagg tttggctgga tctggaggcg
      961 ggagacaatc tcctgcctct gtactttcct ggacatcaga ttcatgcttc ggccgtcaat
     1021 ggcccctatc gcattcactg gcttgctttg tacaccgctg acgcgagtgc cacctatcag
     1081 ttggattaca cgacgaagat ctatgatttt caggatttcg aaggggtcgg actttcctca
     1141 gaggcaactc cgacgccgcc accaacccga aaacctacgc ctacaagtta ccccgatttc
     1201 acaggggact accttgtgaa ctcgctggat cttttccttt tcggtcgctt ctggcgacag
     1261 ccctctccac gggcaccca
//
LOCUS       DAOQUC010001372         1279 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_151750_length_1279_cov_2.969771, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001372 DAOQUC010000000
VERSION     DAOQUC010001372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1279
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_151750_length_1279_cov_2.969771"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(341..754)
                     /locus_tag="PKH07_18415"
     CDS             complement(341..754)
                     /locus_tag="PKH07_18415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNT36970.1"
                     /translation="MIVVDTNVIAFSLIQGDKTKWAQQVRKFDSDWIVPGLWRSEFLN
                     VLATLGKNRILTTEQCLQLWATAETLFSPKERAVDMKDSLVLALKEGITAYDAQYVTL
                     ACSLQVQCVTEDRRLLRRIPSTTICMRDFCTTRHS"
     gene            complement(751..966)
                     /locus_tag="PKH07_18420"
     CDS             complement(751..966)
                     /locus_tag="PKH07_18420"
                     /inference="COORDINATES: protein motif:HMM:NF015807.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HNT36971.1"
                     /translation="MPDILIKNIPSEVHERLKVRARKNRRPMAQELLVILDSTLQQDT
                     RQFPAPVAGNKPLTQNLVTRGIRGGRL"
BASE COUNT          280 a          337 c          348 g          314 t
ORIGIN      
        1 tcctgattcg gtctgtgagc ggccgtctgt cattcatgcg atatcccatt ccctgagaac
       61 gaagttgttc tcaactggag aactctgaga agaatagttc atcctggcct ggcgttcaag
      121 cagtcaggcc aggattctgc catcaagacg aaaatggcct tcggatgacc cctccccttc
      181 cggggttcct tggcttactt ggccaggttc tcccgtacag ggaagtgcag ttcgtggtga
      241 cactgagtcg ggcgcttcaa gcaaagcgga gcctccttca gcttagagca ccctgcagcc
      301 tcgccacttg gcaactggta gcctgagttg gtcttgaaag ttaggagtgc cttgtggtgc
      361 agaaatctct catgcagatc gttgtcgagg ggattcttct cagtaagcgg cggtcttcag
      421 tcacacactg gacctgcaaa gagcaggcaa gggtcacata ctgagcgtcg tatgcggtta
      481 tgccttcctt caacgcgagc acaagcgagt ccttcatgtc taccgcacgt tcctttggcg
      541 aaaacagtgt ctcagccgtt gcccaaagct gaaggcattg ctcggtagtc aggattcgat
      601 tctttccgag agtagcaagg acattcagaa actcagatcg ccacagtcca ggcacgatcc
      661 agtcagagtc aaactttctg acctgctgtg cccacttggt cttgtcgccc tgaatcaggg
      721 agaatgcgat gacatttgtg tccacgacga tcataaccgt cctccccgga tacctcttgt
      781 cacaaggttc tgggtgagcg gcttgttgcc tgcgacaggt gcgggaaact gacgtgtgtc
      841 ctgctgcaga gtactgtcga ggatgacgag aagttcttga gccatgggac gacgattctt
      901 cctggccctg actttgagac gttcgtggac ttccgaaggg atgttcttga tgagaatatc
      961 tggcatggca aaggcctcca cagtactatt tcgacgcggt caatatatct cgggttggag
     1021 tgccggtcaa cgagtattga acgtgtattg tactggctca gttatgcgag gtgatgcaag
     1081 agtttgtagt cacgccgtaa ggcgtgatcc ggatatgccc ttacggacac actacaaacc
     1141 tatggcgcaa gcaagcttgc gcactccaag aatcccgttt gatatgagtc cgtagcagag
     1201 gcgagtcttc gagccgtcgc tgccgacgat ttactactcc cccgctttgg cagcgtcgca
     1261 aagcctctgc aaccgaagc
//
LOCUS       DAOQUC010001373         1278 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_151936_length_1278_cov_3.787408, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001373 DAOQUC010000000
VERSION     DAOQUC010001373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1278
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_151936_length_1278_cov_3.787408"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            28..>1278
                     /locus_tag="PKH07_18425"
     CDS             28..>1278
                     /locus_tag="PKH07_18425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36972.1"
                     /translation="MVLKAIPDEPVLKFNFGSSYSTQVTNKDDFLSYKGGGVNLLGRE
                     DRDIPSDGNFGGAAGLSSTHAPYDGKWSLAAGGKRQVGDITVGGFGSLFYERDSSSYR
                     DGIDDKYWVEHPGDPMTPQYVQGSPDQGDFKTQLFDVQQSAEEVKWGGLGTLGLEVED
                     HALTLLYMYTRTAEDVATLAEDTRGKEFYFPGYDPNDPRHPGNQERDAAPYLRMETLE
                     YTERTTQTLQLRGRHTLLDPDLEIKGGVSFLAPEFDWLVAQSSSGLLQPDKRQFGSLW
                     WAESYNPGYPPYLPPSTYPSLFRPFKPAANFTMGNFQRIWKDISEDSTQYQTNLKIPF
                     EQWSGDEGYLKFGIFKDQLKRSFQQDSFSNFNDNSAQYSGDWDDFWSDVFPDENHPVT
                     AAAMDVDYEGEQDISAWYVMEDVPL"
BASE COUNT          309 a          293 c          374 g          302 t
ORIGIN      
        1 ggtgatgctt cgggcggagc tgtcaatttg gttctcaaag caatccctga cgagccggtg
       61 ctgaagttca atttcggatc cagttacagc acacaggtga ctaataagga tgactttctc
      121 agttacaagg ggggaggagt caacctcctt ggccgggaag atcgagatat cccgtctgat
      181 gggaattttg gtggcgctgc cggactgagc agcacccatg cgccttacga tggaaagtgg
      241 tctttggcgg cagggggcaa gcgtcaggtt ggtgatatta cggtcggtgg ctttggcagc
      301 ttgttctatg aacgcgatag ttcttcctac agggatggta ttgatgacaa atactgggtc
      361 gagcaccccg gcgatccgat gacaccgcaa tatgtgcaag ggtctcctga tcagggggat
      421 ttcaaaaccc agttgtttga tgtgcagcag agcgcggagg aggtgaagtg gggcgggctc
      481 gggacactcg gtctcgaagt ggaagatcac gcattgacgt tactctatat gtatacccga
      541 accgcagaag atgtagcaac gcttgctgag gatacgcgag ggaaggaatt ctatttccct
      601 ggctatgacc ccaacgatcc aaggcatccc ggaaaccaag agcgagatgc agcgccgtat
      661 ctgaggatgg agaccttgga gtatacggaa agaaccactc agacactgca actgcgaggg
      721 aggcacactc tgctggatcc ggatcttgag atcaaggggg gggtgagttt cctggccccc
      781 gaattcgatt ggttggttgc acagagttct tccggactgc tgcagccgga caaacgtcag
      841 tttggttccc tgtggtgggc ggaatcgtac aatcctggtt atccgcccta tctgccccct
      901 tcaacctatc cctctttgtt tcgaccgttc aagccagcag ccaacttcac gatggggaat
      961 ttccaaagaa tctggaagga catctctgag gacagcactc aatatcagac gaatctgaaa
     1021 attccctttg agcagtggtc aggggatgaa ggctatctga aattcggcat tttcaaagat
     1081 cagctgaaac gatcgtttca acaagactct ttcagtaact tcaatgataa tagtgcacag
     1141 tattctggtg actgggatga cttctggagc gatgtctttc ctgacgagaa tcatccggtg
     1201 actgcggcag caatggatgt tgactacgag ggcgagcagg acatatcggc gtggtatgtg
     1261 atggaagacg tgcccttg
//
LOCUS       DAOQUC010001374         1278 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_151945_length_1278_cov_3.513491, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001374 DAOQUC010000000
VERSION     DAOQUC010001374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1278
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_151945_length_1278_cov_3.513491"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            159..>1278
                     /locus_tag="PKH07_18430"
     CDS             159..>1278
                     /locus_tag="PKH07_18430"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNT36973.1"
                     /translation="MQSWKVVLMIFGCLALAWLLAPSRTFEYDDEDVVEITYMGPGGP
                     IAGPMGDSVMAFELESRKAHEKDPSKPVYRVVSGQSGARDQVGDPTRFLISVAGGMPP
                     DVIYFDRFAVAEWAARGAFTPLDSFIENDLRRGDPDAVEPDRYYKAAWDEATYQGKVY
                     GIPISIDNRALLYNKDLFRRAGLVDESGEPVPPKNWTELREYAKKLTQRDRNGNVRVL
                     GFAPNYGNSWLYIYGWMNGARFMSEDGTQCLLNEERVVEALQFMVDIYDDAGGYEAVS
                     AYQAGFQGGTLDPLLTGKIAMKIDGYWQMSFLAQFGRDVDVGVAGAPTSERYFEESGQ
                     PAVSWTGGWSLAIPVNAQHKEAAWEFIRFLSSDRAWRIR"
BASE COUNT          274 a          324 c          395 g          285 t
ORIGIN      
        1 ggggagaaga ggactttttg gtgctccaat ccggacagtc cgtggctcgc gcgagcgact
       61 cgagaattct gactgccggg gatggcactt gaagtttgtg tagaatctcc tatcattcct
      121 gacaatacgt tttgattctt ctcttttcag gggctgcgat gcagagttgg aaagtagtgt
      181 tgatgatctt cggatgcctg gcgctggctt ggctcttggc gccgtcacgc acatttgaat
      241 acgacgacga agatgtggtg gaaatcacct acatgggccc gggcggaccg attgcaggac
      301 cgatggggga ctcggtcatg gcgttcgaac tcgaaagtcg aaaggctcat gagaaagatc
      361 cctccaaacc ggtctatcgc gttgtttctg ggcaaagtgg agcgcgcgac caggttggcg
      421 atccaacgcg cttcctgatc agtgtggcgg gtggtatgcc tcccgacgtg atctattttg
      481 accgatttgc cgtggcggag tgggctgccc gtggcgcgtt cactccactg gactccttca
      541 tcgaaaacga tttgcggaga ggagatcccg acgcggtcga gcccgaccga tactacaagg
      601 cagcgtggga tgaagccacc tatcagggca aggtctacgg catccccatc agcattgaca
      661 accgcgctct tctctacaac aaggacttgt tccgacgagc ggggcttgtg gatgagagcg
      721 gagaacccgt tcccccaaag aactggacgg agcttcgtga atacgccaag aagctgacgc
      781 agcgagaccg caatgggaac gttcgcgtac tgggtttcgc tcccaactat ggaaactcct
      841 ggctttacat ctacggttgg atgaacggtg cccgattcat gagcgaggac ggtacccaat
      901 gtttgctcaa cgaagagcgc gttgtcgaag cacttcaatt catggtggat atctatgacg
      961 atgccggtgg ctatgaggcc gtctccgcct atcaggcagg ttttcaggga ggcacactcg
     1021 atcctctgct gaccggcaag attgccatga aaattgatgg ctactggcag atgtcctttc
     1081 tggcacagtt cggcagggat gttgatgtgg gagttgccgg agcgccgaca tcggaaaggt
     1141 actttgagga gtccggacag cctgcagtct catggacagg cggatggtct ctggcgattc
     1201 ccgtgaacgc gcagcacaag gaagcggcat gggagttcat ccgattcctg agcagcgacc
     1261 gtgcctggcg gattcgga
//
LOCUS       DAOQUC010001375         1278 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_151952_length_1278_cov_3.331971, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001375 DAOQUC010000000
VERSION     DAOQUC010001375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1278
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_151952_length_1278_cov_3.331971"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..453)
                     /locus_tag="PKH07_18435"
     CDS             complement(<1..453)
                     /locus_tag="PKH07_18435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36974.1"
                     /translation="MAQRILGVLSCLVFLSFFGPSAQSELTATAGMQELMPLLPEATL
                     PVGWAQDGKPNWYDKENLFEHNNGDAERFIAYGFRQALVARFAAPQAATSAITVCMYD
                     MGNRENAFGLYSTFRSPDNNFISAGAQGFLASQSVVFWQKQYVVTLEAT"
     gene            complement(477..1277)
                     /locus_tag="PKH07_18440"
     CDS             complement(477..1277)
                     /locus_tag="PKH07_18440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltetrahydrofolate cobalamin
                     methyltransferase"
                     /protein_id="HNT36975.1"
                     /translation="MLIVAENINTSRKQVAPLVENKDKDAIQDIARKVTEAGAGFVDV
                     NCGTFVSQEVELLPWLVSVVQEVTDKPVCVDSPNPKAMEAALAVCRGQVMLNSITAET
                     ARYESLIPMVKKYRTKVIALCMDDAGMPETVEERLNVAKKLVGRLESDGVSRDDIYVD
                     PLVRPIGAVQGAGKMVLDTIWAVMHELKGVHTICGLSNVSFGLPVRKLLNQTFEIMCM
                     TAGLDSVIMNPLDKQQMAQMLACEALLGSDEYCMNYVSAHREGKLELV"
BASE COUNT          299 a          366 c          307 g          306 t
ORIGIN      
        1 cgttgcttcc agagttacga catactgctt ctgccaaaag acgacggact gggaagcaag
       61 aaatccctga gcaccggcac tgatgaagtt gttgtcgggc gagcgaaacg tgctgtacaa
      121 gccgaatgca ttttctctgt ttcccatatc gtacatgcag acagtgatgg cagatgttgc
      181 agcctgtggc gcggcaaatc gagcaacaag agcctgtcgg aatccatagg caatgaaacg
      241 ttctgcatcg ccgttgttgt gctcaaagag attctctttg tcataccaat tcggtttccc
      301 atcctgagcc catccgaccg gcaacgttgc ctcaggcagc aaaggcatta actcttgcat
      361 tcctgctgtt gctgtaagtt ccgactgtgc ggacggacca aagaacgaca gaaagaccaa
      421 gcacgacagg acaccaagga ttctctgtgc catttcacaa cctcccgagt atgacactat
      481 accaattcga gtttgccttc acgatgggca gacacgtagt tcatacagta ttcatcgctt
      541 cccaagagcg cctcacaggc aagcatctgt gccatctgtt gcttgtccag cgggttcatt
      601 atcactgaat ccagaccggc tgtcatacac atgatctcaa acgtctgatt gaggagtttg
      661 cgaacgggga gtccaaagga cacgttggac aggccacaaa tggtgtgaac gcccttcaac
      721 tcgtgcatga ctgcccagat agtgtccaac accatcttgc cagcgccctg aacggcaccg
      781 atgggtcgaa ccagcggatc tacgtagata tcatcacgtg acacgccatc ggactcaagc
      841 cgaccgacaa gtttttttgc aacattcagt cgctcttcga cggtctcggg cattcccgcg
      901 tcatccatgc acagtgcaat gaccttggtc ctgtatttct tgaccatggg aatcaggctt
      961 tcatatcgag ccgtttctgc ggtgattgaa ttgagcatca cctgtccccg gcacaccgcc
     1021 agcgccgctt ccattgcttt cgggttgggg ctgtcaacac acaccggctt gtcggttact
     1081 tcctgaacga cactcacaag ccagggaagc agttccactt cctggctgac aaatgtcccg
     1141 cagttcacat caacaaaccc tgctcccgcc tcggtcactt tgcgggcaat gtcctgaatg
     1201 gcgtccttgt ccttgttctc aacgagcggt gccacctgtt tgcggctcgt gttgatattc
     1261 tcggcaacga taagcatc
//
LOCUS       DAOQUC010001376         1277 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_152152_length_1277_cov_3.622750, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001376 DAOQUC010000000
VERSION     DAOQUC010001376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1277
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_152152_length_1277_cov_3.622750"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1255)
                     /locus_tag="PKH07_18445"
     CDS             complement(<1..1255)
                     /locus_tag="PKH07_18445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36976.1"
                     /translation="MTNLPHVALVNSGTDTRLWYATTTDTFASTGQHVISFTARMPYE
                     RRSNPVYSRITVSAGTDTDTTPIRAILAVDNTPNADLYESFSKLGPYAYSVYLDRFQD
                     SGGVHHPEWIEYYINPFIHSESDGYPSAAAVLNAIDAVGSDIGRLYVHLIGDSTGTGS
                     IEMASGDYLSASDLDLKLDAYQVRSVTGTVVLVVDCPYSGSFLSTCKATGSQKRVLMT
                     SGRSTDSALFSKSGQAVSFSQKILASAYQGNHLRNGFNSGLNFLTTFLKGGIEPQLDD
                     NGDGVYNKKTDGTFAQTLFLGRRYAYAGGEASDLPFVLNVVPAKVHAPGGSAVGFSAD
                     LMEGVEPSRVFAQVIASGVGSVTEPLTTLPEFDFVRDGIELWSWSGNIAVPASDGSYT
                     VAVYASYPDGATQEKISNPKFATILH"
BASE COUNT          297 a          365 c          326 g          289 t
ORIGIN      
        1 gatgcaaaat cgtcgcaaac ttcggattgc tgatcttctc ctgcgtcgct ccgtccggat
       61 aggacgcata gacagcaacg gtataggaac cgtcggatgc aggaacggca atgttgcctg
      121 accatgacca tagttctatc ccatcacgca caaagtcaaa ctccggaagc gtcgtcagcg
      181 gctccgtcac agaacctaca cccgacgcga taacctgcgc aaagactctc gacggctcca
      241 cgccctccat cagatccgca ctgaatccaa cggcactgcc cccgggtgcg tggacttttg
      301 caggaactac gttcagcaca aacggcaagt ccgacgcctc accgcctgca taagcgtacc
      361 ttcttcccag gaagagcgtc tgtgcaaaag tgccatcggt cttcttgttg tagacaccat
      421 ctccgttgtc atccaactgt ggctcgatgc cgcccttcag aaacgttgtc aggaagttca
      481 accctgaatt gaatccattg cggagatgat tcccctgata tgcagacgcc agaatcttct
      541 ggctgaaact caccgcctgc cccgacttgg agaaaagtgc cgagtccgta ctgcgacctg
      601 atgtcatcag tacccgtttc tgggaacccg tcgccttgca ggtggaaagg aatgatccgg
      661 aatacgggca gtccaccaca agtaccactg tccctgtcac tgagcgaact tgataggcgt
      721 ccagtttcaa atccagatct gaagcactga gatagtctcc agaggccatc tcgatggaac
      781 ccgtccctgt gctgtctcct atcagatgca catacagtct gccgatgtcc gacccgaccg
      841 catcaatggc gttcaggact gctgcagcac tcggatagcc atcactctca gagtggatga
      901 atgggttgat gtaatactca atccattcgg gatgatgaac tccgcctgag tcctgaaatc
      961 ggtcgagata aacgctgtac gcatacggtc ccaacttgct gaatgattca tagagatcgg
     1021 cgtttggggt attgtccact gccagaatcg ctcgaatggg cgttgtgtca gtatccgtcc
     1081 ctgccgacac tgtgatgcgg ctgtacactg ggttggagcg gcgttcatag ggcattcttg
     1141 cggtgaaaga aatgacatgt tgtcccgtcg aagcgaatgt gtccgttgtg gtcgcatacc
     1201 acaatcgtgt gtccgtgccg ctattgacca gcgcaacatg gggaagattc gtcacggcgt
     1261 ctccggaaat ggtgctt
//
LOCUS       DAOQUC010001377         1276 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_152349_length_1276_cov_3.384111, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001377 DAOQUC010000000
VERSION     DAOQUC010001377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1276
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_152349_length_1276_cov_3.384111"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            174..>1276
                     /locus_tag="PKH07_18450"
     CDS             174..>1276
                     /locus_tag="PKH07_18450"
                     /inference="COORDINATES: protein motif:HMM:NF014206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HNT36977.1"
                     /translation="MKFARWRETLTARTLCAFGVLLLFSMLGTQNALTQEPLTTTTEF
                     VSSKSLDASFSTRIKDLASVQGTNSLRLFGMGLVVGLAGTGDGQGTEFTIQALVNMLT
                     KRGLTVSADDVRVNNVAAVMVTADLPPFARRGSRVDVMVSTIGDCKSLEGGTLLYSVL
                     QAGDEEVYATAQGALTIGGYNVEGGGGSKVRKNHATTARLTSGGIVEQDFPGSLEENG
                     IVRLSLWRPDFTNASRVARAVDDHFGVAGLATAVDLTTVDVDLGRMTGLYRNLVNLIA
                     ELEQIPVRDDTTARVVINERTGTIVSGADMKLKRVALSHGSLAIYVRGQSVADMLGMP
                     GTSTGNVTVKDEAPRTQIIEEGTTIGEFVDQLAR"
BASE COUNT          296 a          325 c          386 g          269 t
ORIGIN      
        1 cattcgaccc gaagacgtgt cgtctgataa caccgtgcga tcaagcatga tcgccgatgc
       61 ccagatcact tacgacgggg atgggccgat taccgcaaaa gccaaacagg gcttcttgtt
      121 caaattcttt gactggcttc ccatatttta gggttgcgag agaggtacgt gagatgaagt
      181 ttgccagatg gcgagagaca ttgacagccc gaacgctctg tgcgtttggt gttcttcttc
      241 tcttttcgat gttgggcaca cagaacgcct tgacacaaga gcctctcacc accacgacag
      301 agttcgtttc atccaagagc ctcgacgcca gcttctcgac ccgaatcaag gatctggctt
      361 cggttcaggg gacaaacagt ctcagactgt tcggcatggg cttggtggta ggactcgccg
      421 gcactggaga cggtcaggga acggaattca caatccaagc tctggtgaac atgttgacca
      481 agcgcggctt gaccgtcagc gcagatgatg tgcgcgtgaa caatgtcgct gccgttatgg
      541 tcacagcgga tcttccgccg ttcgcacggc gtgggagccg agtggatgtg atggtttcca
      601 ccatcgggga ctgcaagagc cttgaaggag gcacactgct ctacagcgtt ctgcaggcag
      661 gcgacgaaga agtctatgcc acagcccaag gggcattgac catcggggga tacaacgtcg
      721 agggcggtgg cggaagcaaa gtgcgaaaga accatgccac cacggcacgt ctgacttccg
      781 gtgggattgt ggaacaggat ttccccggga gtcttgagga aaacggcatc gtccggctca
      841 gcctttggag accggatttt acaaatgctt cgcgtgtcgc tcgtgctgtg gatgaccact
      901 ttggtgtggc aggtttggcg actgctgtgg acttgacgac ggtggatgtg gacttgggac
      961 gcatgacagg actgtatcgg aaccttgtca acctcatcgc cgagttggaa cagattcccg
     1021 tccgcgatga tacgaccgca cgggtagtga tcaacgaacg aaccggaacc atcgtctccg
     1081 gagcggatat gaagttgaaa cgggtggcac tctcgcacgg cagtctggcg atctatgtca
     1141 ggggacaatc agtggcagac atgcttggaa tgcctggaac ctcaactggg aacgtcacgg
     1201 tcaaagacga agcacctcga acgcagatca tcgaggaagg taccacgatt ggggaattcg
     1261 tcgatcaact ggcgcg
//
LOCUS       DAOQUC010001378         1275 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_152598_length_1275_cov_2.671311, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001378 DAOQUC010000000
VERSION     DAOQUC010001378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1275
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_152598_length_1275_cov_2.671311"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(203..400)
                     /locus_tag="PKH07_18455"
     CDS             complement(203..400)
                     /locus_tag="PKH07_18455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36978.1"
                     /translation="MSATDQNDNQRKEHKAEYCQSCGACPAGQASSRGASTAANEEIE
                     ALVREVTREVLKHLKSVEMKP"
     gene            complement(397..969)
                     /gene="hpt"
                     /locus_tag="PKH07_18460"
     CDS             complement(397..969)
                     /gene="hpt"
                     /locus_tag="PKH07_18460"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018131747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HNT36979.1"
                     /translation="MSTKLMNIDPSGSLGEILFTEEQIASRVRELASQITQDYQGKSL
                     VVMSVLKGSVFFLTDITRHIDLPVELEFVKISTYKGTTQPQVQPRFEHGLPWVHGKNV
                     LLVEDIVDTGETLHYAREALLQNGVASVRICVLLAKEGYQSRGGCVPDYIGFCIPNLF
                     VVGYGLDYQERFRNLPCVAVLHQSGDGESK"
     gene            complement(980..>1275)
                     /locus_tag="PKH07_18465"
     CDS             complement(980..>1275)
                     /locus_tag="PKH07_18465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36980.1"
                     /translation="AQKVYSLLPAGEQGTVDSHLFLARLFYNQKQYTFAMTEARKVQE
                     IDKNNVHAINLKGMVFARRDQLILAIREFEESLSIQPDQPDIQQIVEVLKKSR"
BASE COUNT          281 a          367 c          292 g          335 t
ORIGIN      
        1 ggcatcggca gcctcgatgg cagccacaag acccttggtc tcgatcaatc ccaatgccct
       61 tccttgaatc tcttcagcca cgccttcata cctcctctgt ccgttgttcc ttaggttcag
      121 gaatcaatca cattccttta tggatcagcc tgctatttct gccatatttg tgcccaaact
      181 gtccagcaga aagtgctgtt gatcacggtt tcatttcgac actcttcaga tgcttcagca
      241 cctcacgcgt tacttctcga accaacgcct caatctcttc attggcggca gtgcttgcac
      301 cacggcttga ggcttgtcct gctgggcatg ctccacagct ctggcagtac tccgccttgt
      361 gctcttttct ctgattgtcg ttctgatccg tcgcgctcat ttactctctc catcaccact
      421 ctggtggagc actgccacac aaggcagatt gcgaaaacgc tcctgatagt caaggccata
      481 gcctacaaca aacagattcg gaatgcaaaa accaatgtag tcggggacac atccacctcg
      541 agactggtat ccttccttgg ccagaaggac gcatatacga acgcttgcca caccattttg
      601 aagcagtgct tccctggcgt aatgcagtgt ctctccggtg tccacaatgt cttccacaag
      661 cagcacattc ttcccatgca cccacggcag gccatgctcg aatcgaggtt gcacctgagg
      721 ctgggtggtt cctttgtagg tggaaatctt cacaaattcg agttcgacag gcagatcaat
      781 gtgacgggtg atgtccgtca agaagaacac cgatcccttc agtacgctca tcaccacgag
      841 cgactttcct tggtaatcct gagtgatctg actggcaagt tcacgcacac gcgaggctat
      901 ctgttcctca gtgaacagga tttcacccag agagccgctt gggtcaatgt tcatgagttt
      961 ggtggacact gattcccgtc tacctggatt ttttcagcac ttcaacaatt tgctggatat
     1021 caggctggtc aggctgaata ctgagcgatt cttcaaactc ccggatcgcg aggatcagtt
     1081 gatcccgacg cgcgaagacc atgcctttga gattgatggc atgaacgttg ttcttgtcga
     1141 tttcctgaac cttgcgcgcc tcagtcatcg cgaaggtata ctgcttctgg ttgtaaaaca
     1201 gacgcgccag aaacagatga ctgtccacgg tgccctgctc tccagccggc agcagggaat
     1261 agaccttttg ggctt
//
LOCUS       DAOQUC010001379         1274 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_152741_length_1274_cov_3.360131, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001379 DAOQUC010000000
VERSION     DAOQUC010001379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1274
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_152741_length_1274_cov_3.360131"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..692
                     /locus_tag="PKH07_18470"
     CDS             <1..692
                     /locus_tag="PKH07_18470"
                     /inference="COORDINATES: protein motif:HMM:NF013341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RIO1 family regulatory kinase/ATPase"
                     /protein_id="HNT36981.1"
                     /translation="WTPIMDKSDTQTVRWLLQPPERFKPWIGLLDHSGGVRILKDYSQ
                     KPWYVRKTVGAYALHRESRIYLKLNGIKGIPFFFGFHGSCGIVLEYIAGTVLNKFKGK
                     DLPKVFFDRLELLLQQMHQRGVVHGDLRFKNILVTQDMEPYLIDFATAVSFSDKTIGP
                     WRWFCRYMEKVDRRVLAKLKAKLAPHWLTETDQNLLSEKMFLLNSGRYLRRALSKWYK
                     GEGRKDRKKTS"
BASE COUNT          314 a          325 c          306 g          329 t
ORIGIN      
        1 aatggacacc gatcatggac aagagtgaca ctcagacggt tcggtggctg cttcaaccac
       61 ccgagagatt caagccctgg atcggccttc ttgatcactc tggcggggtg agaattctca
      121 aggactattc tcaaaagcct tggtatgttc ggaagactgt cggtgcctac gctctgcacc
      181 gagaatctcg aatctatctc aaactaaatg gaatcaaggg aataccgttc ttttttggct
      241 tccacggctc ctgtgggatc gtccttgagt acatagccgg tacagtcttg aataagttca
      301 aaggcaagga tcttcccaag gtctttttcg atcgccttga gctccttctt cagcagatgc
      361 accagcgtgg cgtggtacat ggagatttgc gtttcaagaa tatccttgtc acccaggata
      421 tggagcctta tctgatagat tttgccacgg ccgtgtcctt ttcggacaag accattgggc
      481 cgtggcgctg gttctgccgt tatatggaaa aggttgaccg acgcgtcctc gccaaactga
      541 aggcgaagct tgccccccat tggttgacag aaacagatca gaacctgctc tctgagaaga
      601 tgtttctgtt gaattcaggt agatacttgc gtagagcctt atcaaagtgg tataaaggag
      661 aaggtcgaaa agataggaaa aagacttcct gatcgaagaa ctcgaatcag tgagacagag
      721 aatcattgat gctgagcaaa caaacacaac aaggcacttg agctatatcc acctttgtgt
      781 ttgtctggtc agggaatcat agagacaagt cgggcacctt ggtacagtac ctcccgactt
      841 ccggtcctgt gaatttgaac aacccatcag gaccggaagc cgggccaatt gcaggaattc
      901 caaggagagg gctcgccggc cataagcctg gtcagcatct gaacgacgag cgcccatcca
      961 agttttccgt caacaaagtg tcaaggtgcc ttacttcttt ttcgcgactt tcttcgcgca
     1021 ggctttcttc gcacaagcct tcttcgccgc tttcttcacg accttcttgg cggctttctt
     1081 aaccgtcttc gcttttgctg ccacaaccac tcacctcctt ctctgcctga gttcaagcca
     1141 gagcagaatc cagcaatgaa gcaagattct gttttggaat caacccgacg ttgcgggaga
     1201 tttccttacc atccttgaaa atgatcagag ttggaacact catgattccg aatgtcgtcg
     1261 ccgtcttacg tgcg
//
LOCUS       DAOQUC010001380         1273 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_152966_length_1273_cov_3.095238, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001380 DAOQUC010000000
VERSION     DAOQUC010001380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1273
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_152966_length_1273_cov_3.095238"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1005)
                     /locus_tag="PKH07_18475"
     CDS             complement(<1..1005)
                     /locus_tag="PKH07_18475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36982.1"
                     /translation="MRTPRIGWQESQRCLLRIPAHILVISMVLMCLPEGISGFGGMGE
                     NIFSSPLLRVAFCLFMAGFLDLVWLGRIAQCHASQEPGFSTTYSDGNTGKHKPSASEL
                     SGSTEIVSMNDLISLQVDRLRMLAGADTDFAFQSSRHLFTVCVDSEEIRRAMDLMCRV
                     VVQGQQEFGRMIIRTQNVAVEQGFCTRNPWAKPGCYVLLTVSDLDADESRRQWGETSS
                     LDAPTCQFTDDQLARQIKSLFEVVAEHGGFMRIRGKLREGVEIGLFLPIAEALDRDIT
                     QYVERARSQSSGVILLAEDEDFIRELTVKFLKNAGYYVLVAHDGLEARKLFEKYADYI
                     D"
BASE COUNT          323 a          367 c          282 g          301 t
ORIGIN      
        1 atcgatgtag tcggcatact tctcaaagag ttttctggct tcgagaccat cgtgcgcaac
       61 cagaacatag taaccggcat tcttgaggaa tttcacggtc agttccctga taaaatcctc
      121 atcttcggcc agcaaaatca cacccgaaga ctgacttctg gcgcgttcga catactgggt
      181 aatatcccga tccagagctt cggcaatcgg caggaaaaga ccgatttcaa cgccctcacg
      241 caacttgcct ctgatcctca taaatccgcc gtgttcagca accacttcaa agaggctttt
      301 gatctgccgt gccagttgat catcggtgaa ctgacatgtg ggcgcatcga ggctcgatgt
      361 ctcaccccac tggcgccggc tttcatcggc atccagatcg gaaaccgtca acagcacata
      421 gcagcctggt tttgcccaag gattgcgggt gcagaagccc tgctcgacag caacattctg
      481 cgttcggatg atcattctgc caaactcttg ttgcccttga acaacaacac ggcacatcaa
      541 atccatggct cgacgtatct cttccgaatc aacgcagacg gtaaagagat gacgactgga
      601 ttgaaaagcg aaatccgtat cagcacctgc aagcatgcgc agcctatcca cctgcagcga
      661 aatgagatcg ttcatactca caatttctgt tgagccactc aactcgcttg ccgacggttt
      721 gtgtttgccg gtgtttccgt cggagtatgt cgtgctgaat cccggttcct gtgaagcgtg
      781 gcattgcgct atgcgcccca gccagaccag gtcaagaaag cctgccatga acaggcagaa
      841 cgccacgcga agcaacggac ttgagaagat attctccccc ataccgccaa agcctgagat
      901 gccttcagga aggcacatca gaaccataga aatgacaagg atatgcgctg ggatgcgcaa
      961 gagacatcgc tgactttcct gccacccaat ccggggtgtt ctcatttctg cttctccgta
     1021 agcagcccgc agggcaagaa tcgatcgttt tcatccgacc ttctctccag ccaacaacag
     1081 accccactgg ggggcaacaa caaagcaacc cgacttctat tcttgattct atatccgttt
     1141 tctgggttgt caacgccaaa gcacacaaac tcttacttct ccgcacaaac atactcatga
     1201 atctcctttt tccaggagcc gtgtcaactt ggatatatct catgaatatg ctatctacta
     1261 tgctcttggt ttg
//
LOCUS       DAOQUC010001381         1273 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_153053_length_1273_cov_2.155172, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001381 DAOQUC010000000
VERSION     DAOQUC010001381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1273
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_153053_length_1273_cov_2.155172"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1248)
                     /locus_tag="PKH07_18480"
     CDS             complement(<1..1248)
                     /locus_tag="PKH07_18480"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HNT36983.1"
                     /translation="MARGITLTLVERGIQASLIPHGTVNRPVNEVVPLLLDQKAVIDG
                     VFDFVAGRRELSCSYIKEIHAALTQHQRTVQAVDALGNRFDAEILRGTWKQTPNNPTT
                     ASGEIHEYCPPEHVQSEMDRLVQMHLAHIQRRVPPDVEAAWLHHRFTQIHPFQDGNGR
                     VARALASIVFLKSGWFPLALDRSRRSDYIDALEKADHQDLKPLQVLFGQIQKEAFLEA
                     LSLSTDTLREHASLNTVLQATVSRLKQKDSAISEQRKQVLSVAENLAVRTENYLQQVA
                     EDLTSVLRPMRPDSTVTVVRNKPDSTHWFFGQVLELARESGYFADVRTYHAWVRLKIN
                     ELRQAELVVSFHSVGQEFIGLLAASAFLEYREKAEDEETRIVGPYDVARESFQFSYQE
                     DPDLIKQRFDNWLRETIVIGLEQW"
BASE COUNT          316 a          333 c          326 g          298 t
ORIGIN      
        1 ccactgctcc aatccgatca caatagtctc tcgaagccag ttatcaaagc gctgcttgat
       61 gaggtcgggg tcctcctgat atgagaattg gaatgactct cgtgccacat catagggtcc
      121 cacaattcgg gtctcctcat cttcggcttt ctcgcggtat tcaagaaatg cggaagcggc
      181 aaggagaccg atgaactcct gcccaaccga atgaaaagat acaaccaact cggcctgtct
      241 gagttcattg atcttcaagc gaacccatgc atgataggtg cggacatctg cgaaataacc
      301 gctctctcga gccaactcaa gcacttgtcc aaagaaccag tgagtgctgt cgggtttgtt
      361 tcgaacaacc gttacggtgc tatcaggacg catagggcga agtacgctgg tcagatcttc
      421 ggcaacctgt tggagatagt tttccgtccg cactgcaaga ttctcagcga cggaaagaac
      481 ctgctttcgt tgttcactga tggcagagtc cttctgtttg aggcgggaga cggtagcctg
      541 gagaacagta ttgagtgacg cgtgctccct cagtgtatca gttgagagac tgagcgcctc
      601 cagaaaagcc tctttttgga tctggccaaa aagaacctgc agaggcttca ggtcctggtg
      661 gtcagctttt tcgagcgcat caatgtaatc ggaacgccgg gagcgatcca acgcaagagg
      721 aaaccaacca cttttcagaa agacaatgga cgcaagagct cttgcgactc gaccgtttcc
      781 atcttgaaaa ggatgaatct gagtaaaccg atggtgcaac caagccgcct ccacgtcagg
      841 aggaactctc cgctggatat gagcgagatg catctgaacc agacgatcca tctccgactg
      901 aacatgctca ggggggcagt attcgtgaat ctctcccgac gctgttgttg gattgtttgg
      961 tgtctgcttc cacgtccctc gaagaatctc tgcatcaaaa cggttcccca acgcgtcaac
     1021 tgcttgcacc gttcgttgat gttgcgtcag agcagcgtga atttccttga tgtaggaaca
     1081 gctcaactcg cgtctgccgg caacgaaatc gaagacacca tcaataactg ccttttgatc
     1141 gagcaagaga ggaacaacct cattgaccgg tctgttgacc gtgccgtgag ggatgagact
     1201 cgcctgaatt ccgcgttcaa caagggtcag ggtaatcccg cgggccatat cgtatagtcc
     1261 ttcgatgatt ccg
//
LOCUS       DAOQUC010001382         1272 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_153149_length_1272_cov_3.860312, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001382 DAOQUC010000000
VERSION     DAOQUC010001382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1272
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_153149_length_1272_cov_3.860312"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1272)
                     /locus_tag="PKH07_18485"
     CDS             complement(<1..>1272)
                     /locus_tag="PKH07_18485"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36984.1"
                     /translation="GKVSVLALLVLGTYFLIRPTTPYQAYRLQAGDVSSVEILAPMDI
                     EITDEQETQKKQEEARSRVLPVYIESPNVLQRGTEQLEELLNMADAWLQQSTEDQPRL
                     LKHFQESLGLVLSETDMLKLSNDAHQRDIREELPSLFEALYKDGIVESDEDLRSLQQR
                     ARNGLRIKVGANEYRLRVSFSGIRSLEQARDDLAQLIKDRFPARRDSGFLDLLTSVAV
                     SLLEPTLRYDVQDTQRQVEQRLRQVGEAVIRIRQGERIVSSHEQVTEEVMKKLEALKK
                     AYPSFGLINSVGWLMLVLIPYAAGAAYVYKFAPNFWQSGSALTSMALLSFITVALARV
                     ASILPRLGDAFSQSALAVPIGISGILLTVLVNGQLALFWTSVLCVLIAPLLGWDMREI
                     ATLYVGTTFGILSVSGVTKRTDLYKAGFWIA"
BASE COUNT          305 a          350 c          333 g          284 t
ORIGIN      
        1 aaagctatcc agaatcccgc cttatagagg tctgttcgtt tggtgactcc gctcacactc
       61 aaaatcccga aggtagtccc gacatagagc gttgcgatct cccgcatgtc ccaacccaaa
      121 agcggtgcga tcagcacaca aagcacagaa gtccagaaca gtgccaactg tccgttgacg
      181 agaacggtca gcagtatgcc tgatattcca attggcacag cgagggcaga ctgggaaaac
      241 gcatcgccca gccggggaag tatggacgct acgcgagcca aagcaacggt gatgaaggat
      301 agcagcgcca ttgaggtcaa tgcgctgccg ctctgccaga aattgggtgc gaatttgtac
      361 acataggcgg ctcctgccgc gtagggaatc agaaccagca tcaaccagcc aacagagttg
      421 atcaatccaa aagaaggata tgccttcttg agggcttcca gcttcttcat gacttcttcg
      481 gtgacctgct cgtgggaact gacaatgcgt tccccctgtc gaatgcggat gaccgcctcc
      541 ccgacttggc gcaaccgttg ctctacctgc ctctgagtgt cctgcacatc atagcgtagg
      601 gtcggctcaa gcaaagagac agcgactgac gtgagcaaat ccaagaagcc ggaatctctg
      661 cgtgcgggga atcggtcttt gatcagctga gccaagtcgt ctcgcgcctg ctccaatgaa
      721 cggatgccag agaaggaaac gcgaagacga tattcgttgg cacccacctt gatccgaagt
      781 ccatttcgcg cccgttgctg cagactgcgg agatcctcgt cagattcgac aataccgtct
      841 ttgtacaacg cttcgaaaag gctgggaagc tcttcacgaa tatcgcgctg atgcgcatca
      901 ttggacagtt tcagcatgtc cgtttcactc aagaccagac caaggctttc ctggaaatgc
      961 ttcagaaggc gtggctgatc ttcggtggac tgctgcagcc aggcatctgc catattcaga
     1021 agttcttcca gttgttcggt ccctctctgt agaacattcg ggctttctat atagactggc
     1081 agcacccggc ttcttgcctc ttcctgcttc ttctgggttt cctgttcgtc cgtgatctcg
     1141 atgtccattg gagcaagtat ttccacactg gagacatccc cagcttgcag acggtaggct
     1201 tgatagggag tggttggtcg aatgaggaag tacgttccca gaacgagaag cgccaaaaca
     1261 gagaccttgc cg
//
LOCUS       DAOQUC010001383         1272 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_153184_length_1272_cov_2.824158, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001383 DAOQUC010000000
VERSION     DAOQUC010001383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1272
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_153184_length_1272_cov_2.824158"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(943..1164)
                     /locus_tag="PKH07_18490"
     CDS             complement(943..1164)
                     /locus_tag="PKH07_18490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36985.1"
                     /translation="MVKIVSRQEAAMRVANLGLKYAHMLGINSDTDRIRRWTALQKSI
                     LQGDEQTIKWCLSKEALKEFDAEMNQVSI"
BASE COUNT          283 a          375 c          303 g          311 t
ORIGIN      
        1 cacaaccaag atgtgctgcc tctcatcggt tccttaaccg ccccagcgga cagcgtttcc
       61 aaaatccagt ttgtcaatac tgacgaaagg aaacgggaag agtgaaacca tgtagtagat
      121 gttcccatga tctagtacca gacgaacccg agaaagtggt tgtgctttcc cccacatgcg
      181 ttaacacccc caaatggggg caagaagatg atctctcgtt ccatctgtgt atgtcccctt
      241 gatgattcta cgatagaagt aagggtgggg gacactaatg tccctcacag tcacaaacca
      301 tgtaagtaca cctgattcgt tcacttcttg ggcagcgaaa cgcaaatacc gtttcatcgg
      361 tacagtttcc tgtatctcct gcccaccagg ccggttgatc tcagacttcc gcggctggta
      421 cgccacaaca tgatgtgagc ttcggtgaga agacactcgc ctttgaggag gacgtacccg
      481 aggcggcgat tgggttccac gacattcgac ggcaagtgca tattgtcgcc tgcctcgtct
      541 gtccacactg ccccgagttt cacttcggcc ccaaagaggt cgtcgggaac cgctgactag
      601 ccaaccatgc ctgcctgatc gtctccctaa gcgtcttcgg cttctctttt tgggcagccg
      661 tgtcctcccg gtacatttgc accaacgaac gtttgacggc ttccagttcc gcagtggacg
      721 gctcgggttt caactgttct tgcacccatt ccatgaacgc ttggccctga gcaggttccg
      781 ccgaacaacc acatggctgg aaatcggtcc gggagggacc aggaagtgcc tttgtgcttc
      841 ccatagcgaa cactgttgat gtctcaacat gccgagtaat gccttccacc ttggaagaac
      901 agtacggaca caaaaagtct ccgtctaccc catattcgac tttcatattg acacctgatt
      961 catctctgcg tcgaactctt tgagggcctc tttagacagg caccatttga tcgtctgctc
     1021 atcgccctgg aggatgctct tctggagtgc tgtccaacgc cggatccggt cggtatccga
     1081 gtttattccc agcatgtgag cgtacttcaa cccgaggttg gctaccctca tcgccgcttc
     1141 ttgacggctt acaattttga ccattcgatc ttccaatcgg gtgaccacgc cattacatca
     1201 ggcccgccta cgccaactgg cagaagcgca atctgggaac gaactacctc acggatgaat
     1261 ctccgtccgg tt
//
LOCUS       DAOQUC010001384         1272 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_153192_length_1272_cov_2.763353, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001384 DAOQUC010000000
VERSION     DAOQUC010001384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1272
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_153192_length_1272_cov_2.763353"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            128..>1272
                     /locus_tag="PKH07_18495"
     CDS             128..>1272
                     /locus_tag="PKH07_18495"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HNT36986.1"
                     /translation="MKNTMYTILLFGFLSCSTVCLHGDAAEVYMLTHKSSALQKETSL
                     NIILPNGLKQDERYPVLYLLHGAYGCYRDWAERTDIETYADGYEMIIVMPDGGQFGWY
                     VDSPLEATSAYETYITKDLIEAVDRLLPTIASRDARGICGLSMGGHGAMSLAAKHPDL
                     FSSASSLSGILRISAHPEKWHIAARLGSFETASAHWIANSVYDLAQRFLDTDVQLLFD
                     CGVDDTGTGAIQDARDCHERFNELGVQHIYNENPGAHSWQYWDDHIQEHLDFHADNFE
                     KRLGMFVSKRKKIELESNDKWLFLYRDRMAHFRAENQRLKRLFPERKTVVLLGSSTFV
                     AFNEAELLPGWLVLDRGISGDGLGVGDRGILHRLQESVFDCNPAHIF"
BASE COUNT          285 a          310 c          341 g          336 t
ORIGIN      
        1 tgcgttggtc atccgcgtgg ggatcagcca tgacattcac gtcccatgtt tatgagacgt
       61 catgatgtat tgtccggttc ggggcggtca actctggcct gacgcgacct aaggaggaat
      121 atttttgatg aagaacacca tgtatacaat actgcttttc ggttttctct cttgttcaac
      181 cgtctgcctc catggggatg ctgcagaagt ctacatgctc acacacaaga gctcagcgct
      241 gcagaaagag acatcgctca acatcattct gccgaatggt ctgaagcagg atgaacgata
      301 tcccgtgctc tatcttctcc atggtgccta cgggtgctac agagattggg ctgagcgcac
      361 tgacattgaa acctatgcgg atggctatga gatgatcatc gtcatgcctg atggagggca
      421 gtttgggtgg tatgtggaca gtccactcga agcaacatcc gcttatgaga cttacatcac
      481 gaaagaccta attgaagccg tggatcggct cctgcccaca attgcctcac gggacgcccg
      541 tggtatctgt ggcttgagca tgggtgggca tggtgccatg tcgttggctg ccaagcatcc
      601 ggatctattt tcatccgcgt cctcattgag tggtatcctg cgcatttcgg ctcaccctga
      661 gaaatggcat attgccgcgc gccttggttc ctttgagact gcaagtgcac attggatcgc
      721 caacagcgtt tatgatctgg cgcagcgatt ccttgataca gatgtccaac tgctttttga
      781 ttgtggtgtg gatgatacgg gaaccggtgc tattcaggat gctcgcgact gccacgaacg
      841 gttcaacgaa ttgggggttc agcatatcta caacgaaaat cctggtgccc acagctggca
      901 atactgggat gaccacatcc aagagcatct ggattttcat gcggacaatt tcgagaagag
      961 gctgggcatg tttgtgtcca aaaggaagaa aatagaactg gaatccaatg acaaatggct
     1021 tttcctctat cgtgatcgca tggctcattt ccgtgcggag aatcagaggc tcaagcgcct
     1081 cttccccgag agaaagactg ttgtcttgtt gggttcatcc actttcgttg ctttcaatga
     1141 agccgaactc cttcccggtt ggcttgtgct cgatcgaggg atttcgggag atgggctcgg
     1201 tgttggtgac aggggtattc tgcaccgact ccaggaatcg gtctttgact gcaacccagc
     1261 gcatatcttc at
//
LOCUS       DAOQUC010001385         1272 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_153239_length_1272_cov_2.354150, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001385 DAOQUC010000000
VERSION     DAOQUC010001385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1272
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_153239_length_1272_cov_2.354150"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..296
                     /locus_tag="PKH07_18500"
     CDS             <1..296
                     /locus_tag="PKH07_18500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36987.1"
                     /translation="IFATLPSLTTPVAYLTQLPKDPFLGSNYQYFSAVLHPGLETRFQ
                     KLYGDWILLSVGPDKDINLNAISGDLTVYDPSNGTISQGDIIRSQKEGQHGSH"
     gene            283..1197
                     /locus_tag="PKH07_18505"
     CDS             283..1197
                     /locus_tag="PKH07_18505"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNT36988.1"
                     /translation="MEAIEVINVTKTFKNFDAVKEASFCVRQGEIFGFLGENGAGKST
                     TIRMLLGLIWPTEGEVRLNGITVVPGTGEALRGVGAIVEQPCFYGNMTVEQNLRLTAQ
                     LSGGCSQPQIEELLVLLGLDSMRNKRAGIMSHGQKQRLGIAQALLPDNSLLILDEPQD
                     GLDPHWIRETRRLLSLLKQRGVTVLISSHRLNEIEQVCDRVGIIHRGRMLYEGRVEPL
                     LNATEYISISCEPMDKACTLLREHGKNVSPNGNGTLQVHAPTNEQAAQINEMLVSKAI
                     AVSEMSRIRLSLEDVFLNLTGKDAETRQ"
BASE COUNT          319 a          353 c          333 g          267 t
ORIGIN      
        1 gaatttttgc cacacttccc tctttgacga cgccagtagc ctatctgact caacttccca
       61 aggatccttt tctgggaagc aactatcagt acttctcagc agtccttcac ccgggactgg
      121 aaacgcgttt ccagaaactc tatggcgact ggattttgct ttcagtcgga cccgacaagg
      181 acatcaacct caacgccatc tccggcgatc tcactgtcta cgacccatcc aatggcacaa
      241 tcagccaggg ggacatcatc cggagccaga aggaaggcca gcatggaagc cattgaagtg
      301 atcaatgtca ccaagacatt caagaacttc gacgctgtca aagaggcttc cttctgtgtc
      361 cggcaaggcg agatattcgg cttcctcgga gagaatggcg cggggaaaag cacaacgata
      421 agaatgctgc tcggtttgat atggcctacc gaaggggaag tccgcctgaa cggcatcaca
      481 gttgttccgg gaactggaga ggctttgcgt ggcgttggag ccatcgtaga gcagccttgt
      541 ttctacggga acatgactgt cgagcagaat ctgcgtctga cggcgcagct gtctgggggg
      601 tgctctcaac cacaaattga ggaattgctc gtgttgcttg gacttgattc aatgcgaaac
      661 aagcgcgctg gcatcatgtc tcacggtcaa aagcagcgac tgggaatcgc tcaagccttg
      721 ctcccagaca atagcctgct gatcctcgat gaaccccagg acggtcttga tccccactgg
      781 atccgcgaaa caagacgtct gttgagcctt ctaaaacaaa gaggtgtcac cgttttgatc
      841 agcagccacc ggctgaacga gatcgagcag gtgtgcgatc gcgtcggaat tattcaccgg
      901 ggcagaatgc tctacgaagg ccgagtcgag cctctgttga atgccacgga gtacatatcc
      961 atctcctgcg agcccatgga caaggcttgc acactgctca gagaacacgg aaagaacgtc
     1021 tcgccaaatg gcaacggcac actccaggtt cacgccccca cgaatgaaca ggcagcacag
     1081 atcaacgaga tgctggtctc gaaggcaatc gctgtcagcg agatgagccg catacggctt
     1141 tcccttgagg acgtcttcct caatctcact ggaaaggatg ccgaaacgag acaatgagac
     1201 tcatccgcat ggaactgtgc aaactggtgc gacaccggcc actgctgctt tcaacgattg
     1261 ccctgctgct gt
//
LOCUS       DAOQUC010001386         1272 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_153281_length_1272_cov_1.958094, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001386 DAOQUC010000000
VERSION     DAOQUC010001386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1272
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_153281_length_1272_cov_1.958094"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="PKH07_18510"
     CDS             <1..299
                     /locus_tag="PKH07_18510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36989.1"
                     /translation="IRIFQQDAYFSLNFMEKQTQIYRKVLDPVSQKQTITREDADLTE
                     LLEQDALTTELASFVNCVRTRKPPLVGGQEGLTALEMALEITRLVHERLERINL"
     gene            complement(437..>1272)
                     /locus_tag="PKH07_18515"
     CDS             complement(437..>1272)
                     /locus_tag="PKH07_18515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36990.1"
                     /translation="YSRAIGLDIEQWLKCDLVDILTVGGYFRLNPWEYSVQLGHKYGV
                     PVYPSLDETRFQGEAGKIRGCKESYRARATNAWAAGMDGILLFNYYKGKTDPLYNELG
                     DPNRLVGLDKNYTTGARQTGMCKSYLKNGDRFLNCAPVSPENPRTIEPGESVAVELEV
                     GEDLKKSGKPQILLCLQFDGLDETGAVNVSVNENELSQGVIKKIQISGNQPSEEVSSV
                     TSTQRKSTDADGKPAGAGLLEIPLEPEIVTKGMNAIQVGLEASSHQKIELKDILLKVR
                     Y"
BASE COUNT          267 a          353 c          328 g          324 t
ORIGIN      
        1 agatccgcat ctttcaacag gacgcctatt tttctctgaa cttcatggag aaacagaccc
       61 agatttaccg caaggtgctc gaccccgttt cccagaagca gacaatcaca cgagaggatg
      121 ccgacctgac cgaactcttg gaacaggatg cactgacaac cgaattggca tcttttgtca
      181 actgtgtcag aacaaggaag cccccgttag tcgggggaca agaaggcttg actgccctcg
      241 agatggcttt ggagattacc cggcttgttc atgagcggct tgagaggatc aacctctgaa
      301 ccgattctgg tttgcagtct ttcttgtgcc gcagcacgga gttttgccac caaggcgcat
      361 cagcagagca tctcttggcg atacggctaa ttccctgtga ctttccgatc tgtgttggct
      421 gggacagccc cacggactaa tagcgcactt tgagcaggat gtctttgagt tcaattttct
      481 gatgagaaga tgcctccagg ccgacctgaa ttgcgttcat gcctttcgtc acaatctctg
      541 gttcgagtgg gatttcgagc aggccagcgc cagcaggctt gccgtcagcg tccgtgctct
      601 tgcgctgtgt gctcgtgaca gaactgacct cctcggatgg ctggttccct gagatctgga
      661 tcttcttgat caccccttgc gacagctcgt tctcattgac cgagacattg acagcgcctg
      721 tctcatcaag accatcgaac tgcaaacaga gcagtatctg tggctttcca gacttcttca
      781 gatcctctcc gacctccaat tccactgcca ccgattctcc gggctcgatc gttcgagggt
      841 tttcgggaga gacgggagcg cagttcaaga aacggtctcc gtttttcaag tacgacttgc
      901 acattccggt ctgccttgcg cccgttgtgt agttcttgtc cagtccgaca agcctgtttg
      961 ggtcgccaag ttcgttgtag agtgggtcgg tctttccttt gtagtagttg aacaggagga
     1021 tgccgtccat accggcggcc caggcgtttg tggcgcgagc gcgataactc tctttgcatc
     1081 cgcgaatctt ccccgcttcc ccttgaaacc gagtctcgtc caaagaaggg tagacgggaa
     1141 ctccatactt atgacccagc tgaacgctgt actcccatgg gttgagacgg aagtatccgc
     1201 ctaccgtaag gatgtcaacc aggtcgcatt tcagccattg ttcgatgtcg agacctatcg
     1261 cacgcgaata gt
//
LOCUS       DAOQUC010001387         1271 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_153362_length_1271_cov_3.242599, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001387 DAOQUC010000000
VERSION     DAOQUC010001387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1271
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_153362_length_1271_cov_3.242599"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /locus_tag="PKH07_18520"
     CDS             <1..417
                     /locus_tag="PKH07_18520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36991.1"
                     /translation="ALFDIAAGAPGMPALEETRGKGPSIAVAGGVLYGTSYGTDPAIA
                     SRSGLAIDAATRQWVIIRMKVSSGTYADLFYRAPSEVFSAAKRKRFDVTSNTEFVTYT
                     LDMSGAPGWTGTIDRLRLDPTAESGANVQIDYVLIP"
     gene            complement(536..>1271)
                     /locus_tag="PKH07_18525"
     CDS             complement(536..>1271)
                     /locus_tag="PKH07_18525"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNT36992.1"
                     /translation="TARMAKNAVACVVSSHFLKDFLATYNPRTFLIETGVDAEQFRPV
                     NHLERRDDRKVVFLWTGLVWGEDVLQSVLQILEAFQKTASRCPNVGLQIVGGGQRMPD
                     VQEKVRQEYSHLDIQFVGWTAPQDMPALLRQADVGLLPFAGDSKWFRSKSPTKLFEYM
                     ASGLAVVASAVGEITYVIDEGKNGFLVDGAHGFADRMIQLVENPELRAGVGAAAREKA
                     VQDYSLPRLAERLHDMLLQLRNKSLV"
BASE COUNT          319 a          366 c          315 g          271 t
ORIGIN      
        1 gcgctgttcg acattgcggc aggagctcca ggaatgcctg ctctggaaga aactcgtggc
       61 aagggtccaa gtattgctgt cgctgggggc gttctgtacg gaacaagtta tggaaccgat
      121 cctgccattg cctcgcgttc tggcctggca atagatgccg cgacacgtca gtgggtgatc
      181 atccggatga aggtctcttc aggcacctat gcggatctgt tctatagagc gccgtccgaa
      241 gtcttctctg cagccaagag gaagcgcttt gatgtcacca gcaacactga gttcgtgacg
      301 tacacgctcg acatgagtgg agcgcccgga tggacaggga caatagaccg actgcgcctt
      361 gacccaacag cagagtccgg tgcaaacgtc cagatagact acgttctgat tccgtaacga
      421 cgagggcaga atcagaactg cactcacaca gcggattcgt gatggaacaa aaagcccgaa
      481 aggttctacc cagacctttc gggcttttca tctgatccaa atctgcaaat gggctctata
      541 ccagactctt attccgcagt tgtagaagca tgtcgtggag tctctcagca agtcttggaa
      601 gtgagtagtc ctgaacggcc ttctcacgag ctgcagcgcc aacacctgcc ctgagttccg
      661 ggttctccac cagttgtatc atacggtctg caaatccatg agcaccatca acgagaaaac
      721 catttttccc ctcgtcaatg acataggtga tctcaccaac ggcactggcc accacagcaa
      781 gaccggaagc catatactca aaaagtttcg tcgggctctt tgaccgaaac catttagaat
      841 cacccgcaaa tggcaacagg ccaacatccg cctgacgtag cagggcaggc atatcctggg
      901 gtgccgtcca tccaacaaac tggatatcaa gatgagagta ctcttgacgc accttctcct
      961 gaacatccgg cattcgctgt ccccccccga caatctgaag tccgacgttc ggacagcgcg
     1021 aagcagtctt ctggaatgct tcgagaatct gcaaaacgct ttgcaggaca tcctcacccc
     1081 agacaagccc tgtccagaga aaaaccactt tgcgatcgtc cctgcgttcc agatggttta
     1141 ccggtctgaa ctgctcggca tccacgccgg tctcaatgag aaacgtccgc ggattatagg
     1201 tcgccagaaa gtccttcagg aagtggctcg acacaacaca ggcaacagca ttcttcgcca
     1261 tccgagctgt a
//
LOCUS       DAOQUC010001388         1270 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_153560_length_1270_cov_3.361317, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001388 DAOQUC010000000
VERSION     DAOQUC010001388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1270
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_153560_length_1270_cov_3.361317"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1270)
                     /locus_tag="PKH07_18530"
     CDS             complement(<1..>1270)
                     /locus_tag="PKH07_18530"
                     /inference="COORDINATES: protein motif:HMM:NF019325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HNT36993.1"
                     /translation="ARNVYVVFYDGNPAQGGVKFADGTFPVIPTGGQSLAQMNLGGFP
                     KGTHHIYVVIDPENTIPERDENNNVAFNTFVVSALRDLSIASSDITFSPLAPRVGDTV
                     TINATIHNAGSDPSDNVIIQFFVGSPEAGGVQIGSSVKIDRIVANGSGSAQAVWSNVP
                     SSGVFSIYVKVDATESIDESNEDNNMAFRSLAIAEQVDIQLSADGITFSNNSPVEGDV
                     VNIRAVVSNAGLNPASNVRVRFHDGEPGLVEQLWPIQTMGFVAGSAALVDVDQDSKVE
                     VIIGSGDGNLYLLRGDGSNYPGWPVTLGGIVWSSPAVGDINGDAKLDIVVGCVDKKIY
                     AYNLDGSLLSGWPKETNGAILSSPTLADVDGDGQLDVIIGTRGNALHVLKGDGNPVAG
                     FPASVPSWIDSSPTVGDLDGDGQVEIAVGCY"
BASE COUNT          327 a          379 c          296 g          268 t
ORIGIN      
        1 catagcagcc aacagcgatc tcaacttgtc catcgccatc caaatcaccc accgtaggtg
       61 aactgtcgat ccagctggga acgctggcgg gaaatcctgc cactggattg ccatcaccct
      121 tcagcacatg aagggcgttg ccccgtgttc cgatgatcac gtccagctga ccgtcaccat
      181 caacatcggc aagagttgga gaagagagaa tcgcgccgtt ggtctctttc ggccaaccac
      241 ttaacaaact gccatcaaga ttgtaggcat atatcttctt gtccacacaa ccgacgacaa
      301 tgtccaactt tgcgtcgccg ttgatgtctc ccacggcagg agatgaccac acaattccac
      361 ccagcgtcac aggccaacca ggatagttgg aaccatcacc acgcagcagg tacaggtttc
      421 catcgccact cccaatgatc acttccacct tggagtcctg atccacatcc acaagcgcag
      481 cagaaccggc cacaaagccc attgtctgga tcggccacaa ctgctccaca agacccggct
      541 ctccatcgtg gaaccgaaca cgaacattgc tcgcaggatt cagtccggca ttggagacaa
      601 ccgctcgaat gttcaccaca tcgccctcaa cgggactgtt gtttgaaaac gtgatgccgt
      661 cggcagacag ctggatgtcc acctgctctg caatggcaag tgacctgaat gccatgttgt
      721 tgtcttcgtt ggattcatca atgctttcag tcgcgtccac tttgacgtag atactgaaaa
      781 cacccgaaga aggcacattg ctccacacgg cctgagccga acccgaaccg tttgcaacaa
      841 ttctatcgat ctttacagaa ctgccgatct gcactccccc ggcttcggga gaacccacaa
      901 agaactgaat gatgacattg tccgacggat cgctgcccgc attgtgaatc gtggcgttga
      961 tggtcaccgt gtccccgaca cgtggtgcca agggggagaa agtaatgtcc gaagaagcga
     1021 tactcaaatc gcgtaatgct gaaaccacaa aggtattgaa ggcaacattg ttgttctcat
     1081 cacgctccgg aatcgtattc tccggatcaa tgacaacgta gatatggtgc gttcccttgg
     1141 gaaatccacc cagattcatc tgtgcaagag actgtcctcc ggttgggatc acaggaaacg
     1201 tgccatccgc aaacttgaca ccgccctggg caggattgcc gtcgtagaag acaacataaa
     1261 cgttgcgcgc
//
LOCUS       DAOQUC010001389         1270 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_153597_length_1270_cov_2.788477, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001389 DAOQUC010000000
VERSION     DAOQUC010001389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1270
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_153597_length_1270_cov_2.788477"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="PKH07_18535"
     CDS             <1..396
                     /locus_tag="PKH07_18535"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNT36994.1"
                     /translation="IADSIRFTGMVAHEEMPEYLAAADVGVATFEDTEAVSAKSPLKI
                     AEYLASGLPIVASRVGEVSRMVGEAGLLVPPGDSEAVAEAVLRILADSDTIESLGRAA
                     RQRAEAIYNWDQTASNLIQAYRRGIQDYE"
     gene            422..>1270
                     /locus_tag="PKH07_18540"
     CDS             422..>1270
                     /locus_tag="PKH07_18540"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNT36995.1"
                     /translation="MNLPPVLVALAVVQLVVKLLLLNWYRGEYTDAILQLTLFENDST
                     FFAPLFPALAKMTEFVAKDPIYAGRLVSILASTLTLIPIFFIGRRIWSEPAGLLACVC
                     YSVSAIHWRWSVRAMTDPLFALFFLMALLAFVQSFLGRDKMQLWYCLFWTGCTILTRY
                     QGIVLIPLIVFGWIRSRRSSCSSLFMIGGVVPWVFLIWWFFERGFGHTGQFQERAVGG
                     VGNVLMAYLTMAEGFIAYLPYALTYPLFVVMVIGIVKGMALPKARFFGFLTLYLFIPW
                     LVIHSAF"
BASE COUNT          224 a          309 c          368 g          369 t
ORIGIN      
        1 attgctgatt caataaggtt cactggcatg gttgcacatg aggaaatgcc agagtatctg
       61 gctgctgctg atgtgggtgt tgccactttt gaagacacag aagccgtgag cgccaagagc
      121 ccactcaaga ttgcggaata ccttgccagt gggcttccca ttgtggcaag ccgtgttggc
      181 gaggtttctc gcatggttgg cgaggccggt cttctcgtgc ctccaggcga cagtgaggca
      241 gttgcagagg cggtcttgag aattcttgcg gactcagata ctatcgaatc acttggccgt
      301 gcggcgcgac agcgtgccga ggccatttac aactgggatc agacggcatc gaatctgatc
      361 caggcatacc gtcgcggcat acaggactat gaataagcca tctctgcagg acgaccccag
      421 agtgaacctg ccacctgtgt tggtggcact tgccgttgtg caactggttg tcaaactgct
      481 acttctgaac tggtatcgcg gtgagtatac cgatgcgatc cttcagctga cgctgttcga
      541 gaacgacagc acttttttcg cgccactctt tccggctctg gcgaagatga ctgagtttgt
      601 tgccaaagac cctatctacg ccgggcgttt ggtttcgatt cttgcgtcca cactgacgct
      661 cattcccatc tttttcatcg ggcggcgaat atggtctgaa ccggctggtc tgcttgcctg
      721 tgtgtgttat tcggtgtcgg cgattcattg gcgctggtca gttcgcgcga tgaccgatcc
      781 actctttgca ctgtttttcc tgatggcgct gctggcattc gtgcagtcct ttttgggacg
      841 cgacaagatg caactctggt actgtctttt ctggacgggt tgcacgatcc tgacgagata
      901 ccaaggcatc gtcctgattc cactgattgt gttcgggtgg attcgttccc gacgcagttc
      961 gtgttcgtct ctgtttatga tcggtggtgt ggtgccgtgg gtctttctga tctggtggtt
     1021 ctttgagcga ggctttggac atacaggcca gtttcaggag agagcggtag gaggagttgg
     1081 caatgttctc atggcctatc tgaccatggc ggagggattc attgcgtatc ttccctatgc
     1141 gctcacgtat ccgctttttg ttgtcatggt tattgggatt gtcaagggga tggctctgcc
     1201 aaaggcgcgg ttctttggtt ttctgacgct gtatctgttc atcccttggt tggttatcca
     1261 ttcggcgttt
//
LOCUS       DAOQUC010001390         1270 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_153673_length_1270_cov_2.167901, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001390 DAOQUC010000000
VERSION     DAOQUC010001390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1270
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_153673_length_1270_cov_2.167901"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(184..840)
                     /locus_tag="PKH07_18545"
     CDS             complement(184..840)
                     /locus_tag="PKH07_18545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36996.1"
                     /translation="MMCNLTDEERSRIKGEELLRADIRRTIEASTKTESRPILMILNS
                     PFVVTFLGAALIAVLGHLWQQQSIKEQKSLARWEAVQKQKYDLLSSFVQDFESTAMVL
                     GTLYVHRLAFREAKDLQEKSPDDKEVVVKLKEARTAYEKTWSLYQAAPKFNAYTVRVK
                     ALYTDTAVHGALNRLDAAMRNVQKSVTIDDVEKNMGPVNKEFETLATAMGAEISHGDI
                     "
BASE COUNT          268 a          355 c          326 g          321 t
ORIGIN      
        1 caaaaggccg atgcgtaacg ggatccctgt tatcgatctc gcatagcagt tccgagggaa
       61 gaaatccgtg atgatcacgc gagtgatgga atcgtgctac tcgtagaaat cccgtgacgc
      121 ccaattctcg ccacagacct gatggagcga agcatggcta agactatctg ccgggaccga
      181 ctatcagatg tcaccgtgtg atatctccgc tcccattgcg gtcgcaagtg tttcaaactc
      241 tttgttgacc ggtcccatgt tcttctcaac atcatcgatg gtcacgctct tttgtacgtt
      301 cctcatcgct gcgtccaggc gattcagagc accgtggact gccgtatctg tgtagagagc
      361 cttgacgcga acggtgtagg cattgaactt aggcgctgcc tggtagagtg accaagtctt
      421 ctcgtaggcc gtacgggctt ctttgagttt caccaccact tccttgtcat ccggactctt
      481 ttcctgcaga tccttagctt ccctgaaggc aagccggtgc acgtacagcg tgccaaggac
      541 catcgcggtg gattcaaagt cctgcacaaa cgacgatagc agatcatatt tctgcttctg
      601 aactgcctcc caacgagcaa gacttttttg ctcttttatg gattgctgtt gccaaagatg
      661 gcccagaacc gcaatgagtg ctgcgccaag aaacgtaaca acaaaagggc tgttaagaat
      721 catcagaatt ggacgggact ccgtcttggt cgaggcttca atggtccgac gtatgtcggc
      781 tcgcaacagt tcctcccctt ttatcctgct tctctcttcg tcagtcagat tgcacatcat
      841 cgtctccttt gaagttgcag caatgatcat acagtctgcg tactcttcgg cgaggcatcg
      901 tggagggacg acgcgatgtt gcgtggggca gtggggatgc agaggaatcg aagccttcga
      961 caacacccaa aggcttcggc ggcatcttca tcaactcatg tgttcacata cacttgtacg
     1021 cacacacacc acgccctacc actcccggcg attcccatgt cccttcctca ccctgcgttt
     1081 cccggcgtcc ctgcgggcgc atgttccctc agttctctat caacaccagg gatggtcgta
     1141 ggggcccatt tcgtcgattg ggatgggcga ccatacgagg gatctgagtt gcggatcgtt
     1201 gcggatggcc ttctcgctgg acaacgcttg tgggtcgatg gagcgatctg tcactgcgtt
     1261 gatagctgtc
//
LOCUS       DAOQUC010001391         1268 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_153960_length_1268_cov_3.998351, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001391 DAOQUC010000000
VERSION     DAOQUC010001391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1268)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1268)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1268
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_153960_length_1268_cov_3.998351"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..954
                     /locus_tag="PKH07_18550"
     CDS             <1..954
                     /locus_tag="PKH07_18550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36997.1"
                     /translation="ARRIIEDAAEFSLKNDFPLPTQYNFWKSIFRYVPAEEKGTGIPF
                     GFDCAECHNGIEPSQVMDQLGDGTMSRRVLLCTKCRRKKKKCAGCGKLLLNYTSWRSY
                     SQKKHCVELFCRICTMDRVLNLLDKSLETEDFSLTLDDILTLIELSPSPNLMDAVGTI
                     LNRVLNVYPRDPQMPYSYIYERYTDILEKKGQYVQAAENFQKAFDEISAEDELPTKAN
                     IRRLAFLTILAGNKERGTKILTEEISVSPKDFISYETLVQGLMLTKHFREAEKYLNEA
                     FEVARKHGISKGRQKSLLKLKQTLYESFPADLLPRQDLLWE"
     gene            962..>1268
                     /locus_tag="PKH07_18555"
     CDS             962..>1268
                     /locus_tag="PKH07_18555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT36998.1"
                     /translation="MKIGFDALAADNRSGSGVYSRQLLHALARLDSVNEYHIFHTCRV
                     EQLGLGVLPLNFVTHRLLLRARPLRPLWVQTFLPLHAQRLGLDLLHVPQFVAPVWGRT
                     "
BASE COUNT          331 a          316 c          324 g          297 t
ORIGIN      
        1 gcgcgtcgaa tcattgagga tgctgcagag ttttcactca agaatgattt ccctctccca
       61 acgcaataca atttctggaa aagcattttc cgatatgtgc ccgcagaaga gaaaggcacc
      121 ggaatcccgt tcggatttga ctgtgccgaa tgtcacaacg gaattgagcc ttcccaggtc
      181 atggatcagc ttggggatgg gactatgtcc agaagggtac tcctctgcac aaagtgcagg
      241 aggaaaaaga agaaatgcgc cggctgtggc aaactgcttc tgaactatac gagttggcgg
      301 tcttactccc agaagaagca ctgtgtggaa ttgttctgcc gtatatgcac gatggatcga
      361 gttctcaacc ttctcgacaa gtcgctggag acggaagact tctctttgac gcttgatgac
      421 attctgacgc tgatcgagct gagtccttca cccaacctca tggacgctgt tggaaccatt
      481 ttgaaccgag tgctgaatgt ctatcccaga gacccacaga tgccctactc ctacatttac
      541 gaaagataca ccgatatcct cgaaaagaag gggcagtacg tacaggcggc agagaacttc
      601 caaaaagcat tcgatgagat atccgccgaa gatgagttgc ctacaaaggc gaatatccga
      661 agactcgcct ttctgaccat tttggcagga aacaaagaac gtggtacaaa gattctgacg
      721 gaggaaatca gcgtgagtcc aaaggatttc atctcctacg aaactttggt gcaaggactg
      781 atgctgacaa agcacttccg agaggcggag aaatacttga acgaggcttt tgaggtcgcc
      841 agaaagcacg gcatctcaaa agggagacag aagtctctgc tgaaactgaa gcagactctc
      901 tacgaatcct ttcctgctga tcttttgccg aggcaggatc ttctttggga atagagtacc
      961 cgtgaaaatc ggttttgacg cattggcagc agacaacagg agcggaagcg gggtctactc
     1021 acgccaactg cttcacgctc tggctcgcct ggacagtgtg aacgaatacc acatcttcca
     1081 cacgtgtcgc gttgagcagt tgggactcgg cgtcttgcct ttgaattttg tcacgcaccg
     1141 tcttctgttg agagcgagac cgcttcgacc actctgggta cagacatttc ttcccctgca
     1201 cgcacaacgc ctcggattgg acttgcttca tgtgcctcag tttgtggcac ccgtatgggg
     1261 tcgaacgc
//
LOCUS       DAOQUC010001392         1268 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_153978_length_1268_cov_3.441880, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001392 DAOQUC010000000
VERSION     DAOQUC010001392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1268)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1268)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1268
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_153978_length_1268_cov_3.441880"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..640)
                     /locus_tag="PKH07_18560"
     CDS             complement(<1..640)
                     /locus_tag="PKH07_18560"
                     /inference="COORDINATES: protein motif:HMM:NF013161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNT36999.1"
                     /translation="MSKAVREEKCDTTVDRSLYLPISCTLESREVLTPSETLFRLVRN
                     DGQPFGHKPGQFFQISVFGIGEAPISVSSSPTRGDYLDLGVRKAGKLTGMLHESLNPG
                     DEIGVRGPFGTFFDVDAMKGKDLLLISGGCGLAPMRALIQYVEDRSEDFGKVTMLYGA
                     KSPDDVLYKEELALWKKSRIMSCHYTVDSVPGGSCWDSNVGLITKLIPPLEVN"
     gene            complement(790..>1268)
                     /locus_tag="PKH07_18565"
     CDS             complement(790..>1268)
                     /locus_tag="PKH07_18565"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNT37000.1"
                     /translation="IQAGLTAVAAGTLMFVTSLFNGFGRIFWASLSDRIGRRNVFLLI
                     TGTQVPLMLVLPKITSPLVFGAICCYILLCYGGGFATMPSFTADTFGPKYMGRIYGKI
                     LLAWSAAGVVGPMLMEFVKKRTDSFATALTVASGLLVMGFILTLLYRKPQHKAEAA"
BASE COUNT          341 a          360 c          295 g          272 t
ORIGIN      
        1 tattcacttc cagcggagga atcaactttg tgatcaaccc cacattgctg tcccagcaac
       61 ttccacctgg aacagagtcc actgtgtagt gacaggacat tattcttgat ttcttccaca
      121 aagccagctc ctctttgtag agaacgtcgt caggagattt tgccccgtag agcatggtaa
      181 ccttcccaaa atcctctgaa cggtcttcaa cgtactgaat caaagctcgc atgggagcca
      241 ggccacagcc accggagatc agaagcagat cctttccctt catggcatcc acatcaaaga
      301 atgttccaaa gggacctcga acgccaatct catctccggg gtttagactc tcatgaagca
      361 tgcccgtaag tttgcccgct ttccgcacac ccaggtccag ataatcgccc cgagtcggtg
      421 acgaggaaac ggaaattggg gcttcgccaa tgccaaatac ggatatctgg aagaactggc
      481 ctggtttgtg cccaaagggc tgaccgtcat tgcgcacaag acgaaatagt gtttccgagg
      541 gagtcaacac ttcccgagac tccaaagtgc atgagatggg aagatacaga gaacgatcca
      601 ccgtggtatc acatttctct tcccgcacag ccttgctcat tgtttgtgct cctttcgcat
      661 tcaacacctg gtggaaccgt ctccagaaag caggtcaacg gctcagaaca accatcgcct
      721 tccagtgaag aaacatgaca caagtcccca ggtatcagaa tgcatttcgc gctcattgac
      781 agtgggctgc taagccgctt cagccttgtg ctgtggcttg cgatagagca gtgtgagaat
      841 gaaacccata acgagcaagc ctgacgcaac ggtgagtgcc gtcgcaaaac tgtctgtccg
      901 ttttttgaca aactccatca gcatcggacc aaccacgcct gccgcgctcc aagccagaag
      961 tatcttacca tagattcttc ccatgtactt tggcccaaag gtgtccgcgg tgaacgaagg
     1021 cattgtggca aacccaccac catagcaaag aagaatgtaa cagcagattg ctccgaagac
     1081 caacgggctg gtgatcttgg gaagcaccaa catcagcgga acttgcgtgc ccgtaatgag
     1141 aaggaacaca tttcgcctgc cgatcctgtc tgagagcgac gcccaaaata ttcgcccgaa
     1201 accattgaac agagaagtaa caaacatcag tgtaccggcc gcaaccgcag tcagccccgc
     1261 ctggattt
//
LOCUS       DAOQUC010001393         1266 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_154355_length_1266_cov_3.540875, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001393 DAOQUC010000000
VERSION     DAOQUC010001393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1266
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_154355_length_1266_cov_3.540875"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /locus_tag="PKH07_18570"
     CDS             <1..371
                     /locus_tag="PKH07_18570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37001.1"
                     /translation="VVSQFLLARIIVTRKLEKVPFALAGFVGALGIVLMWASNRDILP
                     MLLFNAYLLVLGIGAMRIGITEKSLGKANVGLLIMAILIIARFFDSHLSFTYRGMAFI
                     LIGVGFLVTNLIIKRRMATQ"
     gene            368..925
                     /locus_tag="PKH07_18575"
     CDS             368..925
                     /locus_tag="PKH07_18575"
                     /inference="COORDINATES: protein motif:HMM:NF025703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDYXXLXY domain-containing protein"
                     /protein_id="HNT37002.1"
                     /translation="MKTPKMLMLLVAVALVQLSVPATMIIRREIVLHTGKQFRFRTAP
                     VDPYDAFRGRYVWLSIMPNEAEYTGDRIGRGRKIFLTLKQGDDGFAEVSSVSLSRPKE
                     GDFIQVKSSYAARSNRIGITWPFTRYYMEESAAPEAERVYRDRARRDSSAASVTVRVR
                     RGFGVLEELYIEDLPIKEFLATQKQ"
     gene            complement(984..>1266)
                     /gene="wrbA"
                     /locus_tag="PKH07_18580"
     CDS             complement(984..>1266)
                     /gene="wrbA"
                     /locus_tag="PKH07_18580"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009181555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HNT37003.1"
                     /translation="PAGAFTSGSTMHGGQETTILSFMVTLFHHGMIVVGVPYSEARLL
                     TMKEVSGGTPYGASTIADHDGSRQPTENELAIARFQGKHVAEITRKLRA"
BASE COUNT          286 a          319 c          351 g          310 t
ORIGIN      
        1 ttgtcgtaag ccaattcctg cttgcccgca tcatcgtcac aaggaaattg gaaaaagtac
       61 cattcgcgct ggcaggattc gttggggcgt tggggatcgt gctgatgtgg gcatccaaca
      121 gagatatcct tccgatgctc ttgttcaacg cctacttgct ggttttggga ataggtgcaa
      181 tgaggattgg gatcacggag aagagtctgg gaaaagccaa tgttggtctg ctgatcatgg
      241 cgatcctgat catcgcccga ttctttgact cgcacctctc cttcacatat cgagggatgg
      301 ctttcattct catcggtgtt ggatttctgg tgaccaatct cattattaag cgaaggatgg
      361 cgacacaatg aaaactccaa agatgctgat gctgctggtt gccgttgctc tcgttcaact
      421 atcggttccc gcaaccatga tcattcggcg ggagattgtg ctccacacgg gaaagcagtt
      481 tcgttttcga acggcacccg tggatccgta tgatgcattt cggggtcggt atgtctggct
      541 gagcatcatg cccaatgaag ccgaatacac cggagatcgc attgggagag gaaggaagat
      601 cttcctgact ctgaagcagg gagacgatgg ctttgcggaa gtctcttccg tctcgctgag
      661 cagaccaaag gagggagact tcatccaagt gaaatcgtcg tatgctgcca gatccaatcg
      721 aatcggaatc acctggcctt tcacacgata ctatatggag gagtccgcag ctcccgaagc
      781 ggagagggtc tacagggatc gcgccagacg ggacagttct gccgcttcgg tgactgtaag
      841 agtcagaaga ggctttggag tactggaaga gctttacatt gaagacctgc cgatcaagga
      901 attcctggcc acacaaaagc aatagttcat acaagggagc gctgcgtcag tttccctttc
      961 tcgaaccttg ttcttgaagg ttgtcaggct cttagtttgc gggtgatctc cgcaacgtgc
     1021 tttccctgga accgtgcaat cgccagctcg ttttcggtcg gctgacggct gccgtcatga
     1081 tccgctatcg ttgatgctcc atagggcgtg ccgccagaaa cttccttcat tgtcaacagc
     1141 cgtgcttccg agtagggaac accaaccacg atcatgccgt gatgaaaaag cgtcaccatg
     1201 aaactcagaa tggtggtctc ctggccgccg tgcatggtgc tgcccgacgt gaacgcgccc
     1261 gcgggc
//
LOCUS       DAOQUC010001394         1266 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_154389_length_1266_cov_2.945500, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001394 DAOQUC010000000
VERSION     DAOQUC010001394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1266
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_154389_length_1266_cov_2.945500"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1266
                     /locus_tag="PKH07_18585"
     CDS             <1..>1266
                     /locus_tag="PKH07_18585"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNT37004.1"
                     /translation="VSITYWTGWTGDELRIQERLVQRFNETHPTIHVRIVTLAGVYQK
                     VRIAFAGEDTPDLLSCCWAQEMAGYAVRGVLTPLDAFIARDRPALKEEYFPGLWRSFQ
                     FEGQTMALAVTTNASFFYYNRDIFREVGLDPDWHPKTTDELLETIEKCTIRDPRGNYV
                     RYGYRPSNLEWWAYIFGGEWYDAKTHTVLADHPRNVEALRFLQDFARKHDIRKMQTFE
                     SSMGGFLSTQNPLFVGKAAMTTAGEWFQEHLEDYAPNLNYGYFAAPAPAGGRERCVTF
                     GGSFFVIPAASKHKHEAWQFLNWLTQPEQVKEFCLGIGNLPPLKSVMEEPEFRDNPVF
                     RFAGDLMAGENVFGPPGMPVWTRYVEEINPSEERCVFHNVDPLEDQLVVGDRTFAIDY
                     KNHVRPGAEVPAERLDLQSYIEVIESRIGK"
BASE COUNT          290 a          341 c          351 g          284 t
ORIGIN      
        1 gtttcaatca cgtactggac gggttggaca ggtgatgaac tgcggattca ggaaagactt
       61 gtacagcgtt tcaatgaaac ccatccgacg attcacgttc gcatcgtgac tctcgctggc
      121 gtctaccaaa aagtgcgaat cgcctttgcg ggcgaagaca cgccggattt gctctcctgc
      181 tgttgggcgc aggagatggc cggatatgct gtgcgtggtg tgttgactcc tcttgacgcg
      241 ttcatcgccc gagaccgacc tgcactgaag gaggaatact tcccgggact gtggcggagt
      301 ttccagtttg agggtcagac gatggcgctc gctgtcacca ccaatgcgtc atttttctat
      361 tacaaccgcg acatctttcg cgaggtcggc ttggatccag actggcatcc gaagacaacc
      421 gatgaactac ttgaaacaat cgagaaatgc acgattcgcg atcccagagg aaactacgtt
      481 cgatatggct accggccctc caatctggaa tggtgggcat atatcttcgg cggggaatgg
      541 tatgatgcca agactcacac cgtgcttgct gatcatcctc gaaatgtcga agcacttcga
      601 tttctgcagg attttgcccg caagcatgac attcggaaga tgcagacatt cgagagttca
      661 atgggtggat ttctgtccac acagaatcca ctgttcgttg gcaaagcggc gatgacgaca
      721 gcaggcgaat ggtttcagga gcatctggaa gattatgcgc caaacctgaa ctacgggtac
      781 ttcgccgcac ctgctcctgc gggtggccga gaacgctgcg tcacctttgg cggcagtttc
      841 ttcgtgattc ccgccgcatc caagcacaag cacgaggcct ggcaattcct caactggctg
      901 acccaacctg agcaggtcaa ggaattctgc ctgggcatcg ggaacttgcc gcctctcaaa
      961 tcagtcatgg aagaaccaga gtttcgagac aatccggtct tccgctttgc gggagatctc
     1021 atggcaggag agaatgtctt tggacccccc ggcatgccgg tctggacgcg ctatgtcgag
     1081 gagatcaacc caagtgagga acgctgtgtc tttcacaacg ttgacccgct ggaagaccaa
     1141 ctcgtcgttg gcgacaggac attcgccatc gattataaga accacgttcg tccaggagcc
     1201 gaggtcccgg ccgagcgcct ggacctgcag tcctacatcg aggtcattga atcgcgtatc
     1261 ggcaag
//
LOCUS       DAOQUC010001395         1264 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_154784_length_1264_cov_3.238213, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001395 DAOQUC010000000
VERSION     DAOQUC010001395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1264
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_154784_length_1264_cov_3.238213"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="PKH07_18590"
     CDS             <1..203
                     /locus_tag="PKH07_18590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37005.1"
                     /translation="DFLDPASDNLPVFPDFRRNFRLLPFFRQRISPQNNFSDAKRSKH
                     FCPNLSNEYRELIGSSQPRYLE"
     gene            complement(191..>1264)
                     /locus_tag="PKH07_18595"
     CDS             complement(191..>1264)
                     /locus_tag="PKH07_18595"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNT37006.1"
                     /translation="YSQRAFINDGDEFRFLVHAFVLSFIYVIFFFLGERRLRSSGGVV
                     KCLLILVGFALPLHYRRILQQSHVRSVLGTQIPKEMSRLFAGMETVGQIGHRMLFYTI
                     PGCDVPASLVFLFVLTCALIWLRRSATHRILAGGFAGSLASAFLGLSAALFPFSTGSM
                     ERSAVTLHFCFSGALLLYALYTLTWGKAYTDELTGQANRRALEEAIVRLGRKYSIAMV
                     DIDHFKKFNDTYGHQVGDDVLKFVASRLARARIGKVYRYGGEEFAIVVPRRSAQAVFP
                     LLDGLREGIASSRLVIRTSPRNPALRGTASSAKAQRVSITVSMGVAERTRALPNARAV
                     IESADKALYTAKKQGRNQVVYSK"
BASE COUNT          326 a          355 c          321 g          262 t
ORIGIN      
        1 aggatttcct cgaccccgcg agtgataatc tcccagtctt ccctgatttc cggcggaatt
       61 tccgcctgtt gccattcttt cgccaacgga tttctcctca aaacaacttt tcggacgcca
      121 aacggtcaaa acacttctgc ccgaatttgt ccaatgaata tagggaactg atcggctcga
      181 gtcaaccgcg ctatttggaa tagacgacct gattgcgtcc ctgcttcttg gcggtgtaca
      241 gcgccttgtc agcagattcg ataacggcac gggcattggg aagtgctctg gtgcgttcgg
      301 caactcccat actgaccgtg atcgacaccc tctgcgcttt tgcgctggat gccgtacctc
      361 ggagcgccgg atttcttggc gatgttcgga tgacaagccg gctggaagca atgccttccc
      421 gaagcccgtc aagcagaggg aagactgcct gagcgcttct gcgaggcacg acaatggcaa
      481 attcttcacc accgtatctg tagactttgc cgattcgcgc tctcgcaagc cgactggcga
      541 caaacttcag cacatcgtct ccgacttgat gaccgtaagt atcgttgaac ttcttgaaat
      601 gatctatatc caccatggca atgctgtact tgcgaccaag gcgcacgatg gcttcctcaa
      661 gagcgcgtcg gttggcttgt ccggtcagtt cgtcggtgta tgcctttccc catgtcagag
      721 tgtagagagc gtacagcagc aacgcaccgg aaaagcagaa atgcagcgtg accgcagaac
      781 gctccatcga acctgtcgaa aatgggaaga gggcagctga aagacccaag aatgctgatg
      841 ccagagaacc ggcaaaaccg cctgcgagaa ttcggtgagt cgctgaacgc ctaagccaga
      901 tgagtgcgca ggtaaggaca aacagaaaca caagggacgc tggcacatcg cagccaggaa
      961 tcgtatagaa caacatccga tggccgatct gtccgacggt ctccatgccc gcaaacagcc
     1021 tcgacatctc cttcgggatt tgggtcccca gtacactcct gacatgactc tgctgcagaa
     1081 tcctgcggta gtgcagagga agcgcaaatc cgaccaagat gagcaaacac ttgaccacac
     1141 caccagaact gcgcagacgc ctctcaccaa ggaagaagaa gatgacatag atgaaactca
     1201 atacaaatgc atgaacaaga aaccggaatt catcgccgtc gttgatgaat gcccgctgag
     1261 aata
//
LOCUS       DAOQUC010001396         1264 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_154920_length_1264_cov_1.928040, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001396 DAOQUC010000000
VERSION     DAOQUC010001396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1264
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_154920_length_1264_cov_1.928040"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            380..>1264
                     /locus_tag="PKH07_18600"
     CDS             380..>1264
                     /locus_tag="PKH07_18600"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNT37007.1"
                     /translation="MESSVGVAVVGLGFMGKKHLGVYFGNPRAKVVAVCDSDPEKLHG
                     DWSRCKGNIEDHSESVIDLSDVAKYTNYDSLLKDPNVQVVDITLPSHLHTDFVTRALD
                     AKKNVLCEKPMALTLKECDAMIAAAKANRRRLMVAHCIRFWPEFVVARNLIREGKFGR
                     VCAAHFRRYTGLPEWSWKSWLLDARASGSAVLDLHIHDVDFINSIFGRPLAVTAQGVP
                     TDKRLTGFKRISCLYQVKSGAVVTAEAGWDLPSIVPFEMSFTIVMERVVLTYSSSRQP
                     TLMAHYYDDNEEPLPLPVA"
BASE COUNT          279 a          328 c          342 g          315 t
ORIGIN      
        1 agaatgttgt tgaaatcatg ggcgatgcaa ctcaccaagg tctgcatgac ctcgattgga
       61 tctgccgact tctcgttctt catcgccaac acctttccac caactgctgc tatcgctccg
      121 ccaagttcgc ccattttcaa cttcgattca cagtctatca atttcgttca gacaaaacaa
      181 cagcacagga cgatttcaga ctgtgtcttg acacgactca gccaaacagg gtaagcatag
      241 agggctgcct tgggtgagac ggtctgtctc gatctcgccg ggagcgggtt tcaggcagtt
      301 ctactcggca gaacgggtgc caagagcaca ggcgtgagtg cccgtcttgt ttgcatcatg
      361 acacagggga ggtcttctga tggagagttc agtcggagtc gccgttgtcg ggcttggctt
      421 catgggcaag aagcatctgg gcgtttattt tgggaaccct cgtgcaaagg tggtcgctgt
      481 ctgcgattcg gatcctgaga aactccacgg agactggtca cggtgcaaag gcaatatcga
      541 agaccactct gaatcggtta tcgatctgag cgatgtcgcc aagtatacca actatgattc
      601 attgctgaaa gatcccaacg tgcaggttgt ggacatcact ttgccgagtc atctgcacac
      661 cgattttgtt accagagcgc tggacgcaaa gaagaacgtc ctttgcgaga agcccatggc
      721 actgacgctg aaggaatgcg atgccatgat cgccgccgcc aaagccaaca gaaggcgtct
      781 catggtcgcc cactgcattc gtttttggcc ggagtttgtc gtggctcgaa atctgattcg
      841 tgaagggaag ttcggcaggg tgtgtgccgc gcacttccga cgctacaccg gtctgccgga
      901 atggagttgg aagagttggc tcttggatgc gagggcatcg ggttccgctg tattggatct
      961 gcacattcat gatgtggatt tcatcaactc cattttcggt cgtccgcttg ccgtcacagc
     1021 gcaaggtgtt cccacagaca aacgactgac aggattcaag cgaatctcgt gcctctacca
     1081 agtcaagtct ggtgctgtgg tcactgcgga ggcgggttgg gatctaccca gtatcgtccc
     1141 gtttgagatg tctttcacaa ttgtcatgga gcgtgtcgta ctgacgtatt cttcctctcg
     1201 gcaaccaaca ctcatggctc attactatga cgacaatgag gaacccctgc cacttcctgt
     1261 ggct
//
LOCUS       DAOQUC010001397         1263 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_155018_length_1263_cov_3.445364, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001397 DAOQUC010000000
VERSION     DAOQUC010001397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1263
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_155018_length_1263_cov_3.445364"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..157
                     /locus_tag="PKH07_18605"
     CDS             <1..157
                     /locus_tag="PKH07_18605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020879061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HNT37008.1"
                     /translation="IGDKYVQISPGGSDVILENGDTIVETESAVDLEALISKYAFGKV
                     DKEDEQE"
     gene            168..845
                     /locus_tag="PKH07_18610"
     CDS             168..845
                     /locus_tag="PKH07_18610"
                     /inference="COORDINATES: protein motif:HMM:NF017319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNT37009.1"
                     /translation="MKKTSLGVLVLCFLASGALVVRAEDSAPAERIPPDQVLLDAINR
                     TLDIVRLPDYKAPEKRAEYRAQVREILMTMIDMRTVSMLVLADKKKAFTDAQFDEFVD
                     SFTRILFLSYVGHLDDYTDQQVQVDSVEEIKDSKVPRVRLKTQTTSRAKGTKTPVEYS
                     MTLTDNKWAAYDVRIEGLSFVSNYRSQFREILTSRKPDQLLKTMKEKADKLEKDNVQI
                     KVDEKIF"
     gene            829..>1263
                     /locus_tag="PKH07_18615"
     CDS             829..>1263
                     /locus_tag="PKH07_18615"
                     /inference="COORDINATES: protein motif:HMM:NF016236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaA family lipoprotein"
                     /protein_id="HNT37010.1"
                     /translation="MRRFSRNLGLFVTVSCILFYSSAVLAEEPPVTEEPTTEIEQDWT
                     DFQDEYTGEAATRIGDPLRGMNRTFYHFNDIMMVWFITPVSEVWGFILPQFARFRIQD
                     LVDNLGFPRRFLNTLFQGRPGRAGIELSRFVVNCSLGLGGLWD"
BASE COUNT          312 a          314 c          341 g          296 t
ORIGIN      
        1 tatcggcgac aagtacgtcc agatctcacc gggaggctct gacgtgatac tcgaaaatgg
       61 cgacaccatc gtcgagaccg aatccgccgt ggacttggag gcgttgatca gcaagtacgc
      121 gtttggcaag gtagacaaag aagacgaaca ggaatgaggt aaccctagtg aagaagacat
      181 ctttgggcgt tctggttctc tgctttttgg cgtcgggtgc tctggtcgta agggcggaag
      241 attctgctcc cgctgagcgt atcccacccg atcaggtact cctggatgcc atcaatcgga
      301 cactggatat cgtccggctg cctgactata aggcacccga gaagcgtgct gagtaccgtg
      361 cacaggtcag agagattttg atgactatga ttgacatgag aacggtctca atgcttgttc
      421 tggcagacaa gaagaaggcc ttcactgacg cacagttcga cgaattcgtg gacagtttca
      481 cccgtatcct tttcctttcc tacgtcgggc atcttgacga ctacaccgat cagcaggttc
      541 aggtggatag cgttgaggaa atcaaggatt ccaaggtgcc tcgcgtgcgc ctgaaaacac
      601 aaaccacttc ccgagccaag ggaacgaaga cccctgttga gtacagcatg acgctgacag
      661 acaataagtg ggcagcctac gatgtgagaa tcgaaggcct gagcttcgtc tccaactacc
      721 gatcacagtt cagggaaatt ctgacctcaa gaaaacctga tcagcttttg aaaacgatga
      781 aggagaaggc agacaaactt gagaaagaca acgttcagat taaggttgat gagaagattt
      841 tctaggaatc tcggcctatt cgtcacggtt tcctgcatcc ttttctactc ctccgccgtc
      901 ttggcagagg agccaccggt caccgaagag ccgaccacag agatcgaaca ggattggact
      961 gattttcagg acgagtacac gggtgaagcc gccacgcgta tcggggatcc actccggggg
     1021 atgaaccgca ctttttacca cttcaacgac atcatgatgg tctggttcat cactcctgtt
     1081 tctgaagtct gggggttcat acttccccaa ttcgcaagat tcaggattca ggatcttgtg
     1141 gacaatctgg gctttccgag aaggtttctt aacacgctgt tccaaggtcg cccgggacgt
     1201 gctggcatcg agctgagtcg ttttgttgtc aattgcagcc tcggtctcgg tggtctgtgg
     1261 gat
//
LOCUS       DAOQUC010001398         1262 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_155215_length_1262_cov_4.298260, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001398 DAOQUC010000000
VERSION     DAOQUC010001398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1262
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_155215_length_1262_cov_4.298260"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="PKH07_18620"
     CDS             complement(<1..282)
                     /locus_tag="PKH07_18620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37011.1"
                     /translation="MQVRQNRVVERMRAGKYVLFFGNTPFITPKLVEMAGLIGFDGVW
                     IDMEHQDYHYHDVSNMCLACRATGMEPMVRIRKSGDHAVYRAFEAGATGI"
     gene            complement(480..>1262)
                     /locus_tag="PKH07_18625"
     CDS             complement(480..>1262)
                     /locus_tag="PKH07_18625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37012.1"
                     /translation="AWTGWVGITDYSGLPYPYQRYYFSLARQAFNERPDRDSEALLHA
                     AKAAVVIPYGYTFAPYHMHRTIWLPLEKENDFGVTYRRVLANAVTEAERLLRLGIDFD
                     VAVNDPLFIPTGYEELIYAQEDGKIRVSQPERADEILDAPRFFERPDLGPAPSLSIEI
                     EGDVPPGPGEVTFKAVATIGTGDWSGEGEEPKIYWEIYDEESIIELKYSALWQTQGER
                     LTYEFESEGTYVVRASVCDIFGRPAVAYKAIVVGNSAVKETQ"
BASE COUNT          297 a          367 c          317 g          281 t
ORIGIN      
        1 aatgccggtt gcaccagcct cgaaagctct gtaaacggca tggtcaccgg atttcctgat
       61 gcgcaccatg ggctccatac ccgttgctcg acacgccaaa cacatattgg acacgtcgtg
      121 gtagtggtag tcctggtgct ccatgtcaat ccagacgcca tcaaaaccga taaggccagc
      181 catctcgacc aatttgggcg tgatgaatgg cgtattgcca aaaaacagaa catactttcc
      241 tgcccgcatt ctctcgacaa cccgattctg ccgcacttgc atcaacgtaa cttcctttca
      301 caacatgaat gatgactgtt gctgagtctc tgctcgacaa acacacgtca gagtcatgcc
      361 aacgcatggc gaggacacat cctatgacat gtgatgaaga agatatacca atgtccctaa
      421 gaagtccagc gacgcaacgg agtttccgtg cccgcgatcc tatcgcacgg cgtgctcgat
      481 cactgagtct ctttgacggc ggagtttcct accacgatag ccttgtaggc gactgccggt
      541 ctgccgaaaa tgtcgcatac cgaggcacgc actacatagg tgccttcaga ttcaaactcg
      601 taagtcaacc gctccccttg ggtttgccac agagcggagt acttcaattc gatgatgctc
      661 tcctcatcgt agatctccca gtagatcttg ggttcttccc cctctccaga ccagtcccct
      721 gtgcctatcg tggccactgc tttgaatgta acctccccag ggcctggtgg cacatcgcct
      781 tcaatttcaa tggaaaggct tggcgcgggc cccaggtcgg ggcgctcaaa gaaacgagga
      841 gcgtcgagaa tttcgtcggc tctttccggc tgcgagacgc gaatcttgcc atcctcctga
      901 gcgtagatga gttcctcgta acccgttggg atgaacaacg gatcatttac cgcgacgtcg
      961 aagtcaatgc ccagacgcag aaggcgttcc gcttccgtca cagcgttcgc cagaacccgg
     1021 cgataggtca ccccaaaatc gttctccttt tccagcggga gccaaatggt tcggtgcatg
     1081 tgatagggag caaaggtgta gccgtaagga atgacaacgg cagcctttgc agcatgcaac
     1141 agtgcttctg agtcacgatc cggacgctcg ttgaacgcct gacgtgccag tgagaaatag
     1201 tagcgctgat agggataagg aagtccggag tagtcggtga ttcccaccca tcccgtccaa
     1261 gc
//
LOCUS       DAOQUC010001399         1262 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_155274_length_1262_cov_2.610605, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001399 DAOQUC010000000
VERSION     DAOQUC010001399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1262
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_155274_length_1262_cov_2.610605"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1119
                     /locus_tag="PKH07_18630"
     CDS             <1..1119
                     /locus_tag="PKH07_18630"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HNT37013.1"
                     /translation="QLVAESVKVGYDVRPRRLDPLCEFQNGTLLAFVTDRADQGETLL
                     TMATDPLPEIVLRGVDDATMTVFLDRFYVEGKASSGQQIASLKINGREMMNESALEVH
                     FASVVDLQEATNTIQIAASDKNNVLSERTVQVLRVVPDAQSLGTRLSLTISPLEVMGE
                     ASQAGSLIEDRMMRAFVAPKRFRILDRASLDTILLERKLSATDLADPSTAAQMGRLQD
                     AEFILTGFVRHRVAESGLEIMGRLVETETGRVLFEHDVYDESVQGDGLSQMLEGLYLK
                     FARHFPLLGGPVLEAGRQGVSVELPYTGLARAGMRFVVYREGKQLVHPVTGRVLGAAT
                     TILGEAIIERTVENGAFMKVLDGVRVSSLSPGDRVIAR"
BASE COUNT          261 a          277 c          396 g          328 t
ORIGIN      
        1 caactggtcg ccgaatctgt gaaggtcggc tatgatgtgc gccccagacg gctggatcca
       61 ctctgtgaat tccagaatgg tacgcttctg gctttcgtga ctgatcgtgc agatcagggc
      121 gaaacattgc ttacgatggc aacggatccc ttgccggaga tcgttctgcg tggagtggac
      181 gacgcaacga tgaccgtgtt cttggatcgt ttttatgttg aaggtaaagc gagcagtggg
      241 cagcagattg cgagcctgaa gattaacggc cgggagatga tgaatgagtc tgcacttgaa
      301 gtccactttg ccagtgttgt cgatcttcaa gaggcgacca acaccattca gattgccgca
      361 tcggataaga acaacgtcct tagcgaacgg acggttcagg tgttgcgagt tgtgccggat
      421 gcccaatccc ttggaactcg attgagcctg actatttcac ctctggaagt catgggagag
      481 gcttctcaag cgggttctct cattgaggat aggatgatgc gtgcctttgt tgcaccgaag
      541 aggttccgaa tccttgatcg cgcgagtctt gatacgattc tcctggaacg aaagctttca
      601 gcaacggatc tggctgaccc ttcgactgcg gcgcagatgg gtcgtctcca ggatgccgag
      661 ttcatcctca caggttttgt tcggcaccgg gttgcagagt ctggattgga gatcatggga
      721 aggcttgttg agacagagac aggtcgagtg ctcttcgagc acgatgtcta tgatgaaagt
      781 gtgcagggcg atggactttc tcagatgctt gagggattgt atctgaagtt tgcccgacac
      841 ttccccttgc ttggcggacc ggtgcttgag gcggggcgtc agggagtgtc cgttgaactg
      901 ccttatacag gtttggcgcg tgctggaatg cgcttcgttg tctaccgtga aggcaagcag
      961 ttggttcatc ctgtcactgg gagagtcctt ggtgcagcaa cgacgattct gggtgaagcg
     1021 atcattgaga gaacggttga gaacggggct ttcatgaagg ttctggatgg ggtgagagtg
     1081 tcaagtctaa gtcctggcga tcgagtcatc gcgcgatagg aggctctggt gaagaggcat
     1141 cttctcttgt tctgttcagc atccattgcg ctgacgatgg ggctttggag ttgtgcagat
     1201 gagatgagtc agtcccactc ggatgaggag tgttcctcca ttgttgttga gaggcatttc
     1261 gg
//
LOCUS       DAOQUC010001400         1259 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_155866_length_1259_cov_4.009967, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001400 DAOQUC010000000
VERSION     DAOQUC010001400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1259
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_155866_length_1259_cov_4.009967"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            168..1226
                     /locus_tag="PKH07_18635"
     CDS             168..1226
                     /locus_tag="PKH07_18635"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HNT37014.1"
                     /translation="MATYKIAVIPGDGIGPEVAAEAIKVAQAAAKKYGCKLELTYFNW
                     GGDHYLATGELLPEDALDQLKKFDAMYLGAIGHPKVKTGVLEKGILLNLRFGLDQYIN
                     LRPVKLYPGVWTPLKDKGPKDIDFVVVRENTESLYVGIGGFFKKGTPDEVAIQEMVAT
                     RKGVERAVRYAFEFCRKNGRKKVTLCDKSNVLTYAHDLWQRVFWEVGEEYPALEKDHA
                     HVDAITMWMVKNPEWFDVIVTDNMLGDIITDLGAMIQGGMGIAAGANLNPEGLSMFEP
                     IHGSAPKYAGLNKANPLAAIASAGLMMENLGEKKAGDAIEQAIIKALGEGHIKTLSAG
                     GEKSTSEIGDLISEFVSE"
BASE COUNT          310 a          289 c          364 g          296 t
ORIGIN      
        1 gattcctcac tacgcgcgaa gacttgcttc gttcggaatg acggttcact tcatcgctcg
       61 cagaactgag ctggtacacc tcggcaaaga aaactcttcc ctgtaagggt gtgttttggt
      121 atctttccag ttcgactgaa aaacgaactg aatttttgga ggatgaaatg gcaacgtaca
      181 agatagcggt catacccgga gacggcatcg ggccggaggt tgctgcagag gcgataaagg
      241 tcgcccaggc tgcagcaaag aaatacggat gcaaactcga gttgacatat tttaactggg
      301 gtggcgacca ctatctcgca acgggcgagt tgcttcctga ggatgccctg gatcagctga
      361 agaagtttga tgccatgtat ctgggagcca ttggtcatcc caaggtcaag acgggcgtct
      421 tggagaaggg cattctgctg aatcttcgat ttggtctgga tcagtacatc aacttgcgtc
      481 ccgtgaaact ctacccgggc gtttggactc cgctcaaaga caaggggcct aaggatattg
      541 attttgttgt ggttcgtgag aatacggaga gcctctacgt cggcatcggc ggattcttca
      601 agaagggtac tcctgacgaa gtcgccattc aggagatggt ggcgacgaga aagggtgttg
      661 agcgcgccgt tcgttacgct ttcgagttct gccgaaagaa cgggcgcaag aaagtcacgt
      721 tgtgcgacaa gtcaaatgtc ttgacatacg ctcacgacct ctggcagcga gtcttctggg
      781 aagttggtga ggaatatccg gctctcgaga aagaccatgc ccatgtggat gcaatcacaa
      841 tgtggatggt caagaatccc gaatggtttg atgtcatcgt cacagacaat atgcttggcg
      901 acatcatcac cgacctgggt gccatgatcc agggtggaat gggcattgct gctggggcaa
      961 atctgaatcc tgaaggactg tccatgtttg agccgatcca tggctctgca ccaaaatatg
     1021 ctgggctgaa caaggcgaat cctctggctg ccattgcgtc cgctggtctg atgatggaga
     1081 accttggcga gaagaaagcc ggggatgcca tcgagcaggc gatcatcaaa gccctcggcg
     1141 aaggtcatat caagactctt tccgccggtg gcgaaaagag caccagtgag atcggtgact
     1201 taatttcaga gttcgtttcg gagtaaagat ccgattcggg ttgttcacct attcagaga
//
LOCUS       DAOQUC010001401         1258 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_156089_length_1258_cov_3.968412, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001401 DAOQUC010000000
VERSION     DAOQUC010001401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1258
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_156089_length_1258_cov_3.968412"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..623
                     /locus_tag="PKH07_18640"
     CDS             <1..623
                     /locus_tag="PKH07_18640"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HNT37015.1"
                     /translation="IWLAIKTPGLGVPEMGAIVCFALFFWGHTIAGLAGWESFLLFLV
                     GAVLLFIELFVTPGFGIVGLSGITLIVTSLVLTLAEHPINSAFFRFPDLLPPFYLLAW
                     SMFGAIVLGLALARFLPKTSFYQRLVLAHSEKRSEGYHSSEDKALLVPIGATGVSTSV
                     LRPSGMARFEQGRFDVVTQGDYIPSGVPVEVIRIEGRRLVVRRLQT"
     gene            623..1111
                     /locus_tag="PKH07_18645"
     CDS             623..1111
                     /locus_tag="PKH07_18645"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HNT37016.1"
                     /translation="MLWMIVVLMVLGLLFLFLEVFLPGGVTGIIGSVMLLVGVILSFV
                     HFDEVQAFSILVGTVLISVMIMVLGVKIFPRTRLAGRIILREGLTRDRGYTPSSEEHE
                     SLVGLSGVAISTLRPSGIADFDGRRVDVVTEGDFIREGERVVVVGADGNRVVVERQNS
                     VG"
BASE COUNT          227 a          265 c          371 g          395 t
ORIGIN      
        1 gtatctggct tgccatcaag actcccggac ttggtgtacc ggagatgggt gccatcgtgt
       61 gttttgctct cttcttttgg gggcatacca tcgccggcct tgcgggatgg gaatcgtttc
      121 tgctttttct cgttggggca gtgcttctat tcattgaact tttcgtcaca ccaggatttg
      181 gaattgtagg tttgtccggg atcactttga tcgtgaccag tcttgttctc acgctggcag
      241 agcatccgat caacagtgca tttttccggt tcccggatct tctgcctcca ttttatctcc
      301 tggcctggtc catgtttgga gccatcgtac tcggtttggc gctggcaagg ttcttaccca
      361 agacaagttt ctatcagagg cttgttctgg cgcactcgga aaagagaagt gaaggctatc
      421 acagttctga agacaaggcg cttctggttc cgataggcgc tacaggcgtc tccacaagcg
      481 tgcttcggcc ttctggaatg gcgagattcg aacagggccg tttcgatgtc gtcacacagg
      541 gcgactatat tccttcaggc gttccggttg aagtcattcg gattgagggt cgaagattgg
      601 tcgtacggag gcttcaaaca taatgttgtg gatgattgtt gtactgatgg tgctgggtct
      661 tctctttctc tttctggagg tgttccttcc cggtggcgta acggggataa ttggctccgt
      721 catgcttctg gtcggagtga ttctgagttt tgtccacttt gatgaggtgc aggcctttag
      781 catacttgtt gggacggttc tgatttcggt gatgatcatg gttctgggtg tgaagatttt
      841 ccccagaaca aggctcgcag ggcggatcat tcttcgtgaa ggattgactc gtgatcgagg
      901 ttacacccct tcaagtgagg agcatgagtc acttgttggg ctctcaggtg tcgcgatctc
      961 gacattgcga ccttccggaa tcgctgattt tgatgggcgc agggtggatg ttgtcaccga
     1021 gggggacttc attcgagaag gggaacgggt tgtcgttgtt ggggcagatg gaaacagagt
     1081 tgttgttgaa cgccagaata gtgtaggata gtacagatat tgaaaattgg ctttgggagg
     1141 ttcgaaaatg cctgttggat tcttcgctgc tgtatttgtg tttgtaatca tttttgcgtt
     1201 cctgtttttg atgtggttgg tacctttgcg gctttggatc gccgccatgg cgtcaggt
//
LOCUS       DAOQUC010001402         1258 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_156110_length_1258_cov_3.251870, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001402 DAOQUC010000000
VERSION     DAOQUC010001402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1258
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_156110_length_1258_cov_3.251870"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1040)
                     /locus_tag="PKH07_18650"
     CDS             complement(<1..1040)
                     /locus_tag="PKH07_18650"
                     /inference="COORDINATES: protein motif:HMM:NF021570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2298 domain-containing protein"
                     /protein_id="HNT37017.1"
                     /translation="MALQFLTALKMYLTVSFLGLLGIPLCFLVFSRIRGGAVLFARPA
                     MLLLITYLVWIASTIHLLPFTRLGIVIAALIILGATAPLLLKKREQLLERLREDLPII
                     WRGELLFLIVFLVAVFFKMHKSITYGTEKYMDLALLSSIFRTRWMPPADPWLSGHSIN
                     YYYGGYMIFAGLAKLAGVSAAVGYNLAIALTFALVAVCIYTLVYSLTKSHWLGLLGCV
                     LHSWIGNLEAARQVTRHLLTGFASQISSPTRIFVWFFSAFHPINLWKTTRVIVDATGG
                     ETINEYPLFTFYWGDLHPHLTSMPFVILVLALIWQLLKSPEKGTLTQRLKKDLPGSLF
                     LSLSLGGLALING"
     gene            complement(1068..>1258)
                     /locus_tag="PKH07_18655"
     CDS             complement(1068..>1258)
                     /locus_tag="PKH07_18655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37018.1"
                     /translation="EILHSARRLVEPVLSLRRILNDKCLKHPRLTEPAQRVFAGMCLS
                     GIDILSGKTVFYPRSVAA"
BASE COUNT          371 a          331 c          307 g          249 t
ORIGIN      
        1 ccattgatca gggcaagccc acccaaagag agtgaaagaa aaagagaacc tggcaagtcc
       61 ttcttcaacc gctgcgtgag agttcccttc tctggcgact tcagcaactg ccagatcagc
      121 gccagcacca aaatcacaaa tggcatcgaa gtcagatgag ggtgcaaatc cccccagtaa
      181 aatgtgaaca gaggatactc gttgatcgtc tcgccccctg tggcatccac aatgactctt
      241 gtggtcttcc agagattgat cggatgaaag gcagagaaaa accagacgaa gattcgcgtt
      301 ggactggata tctgtgaagc aaatccggtg agaagatgcc gagtcacttg tctggccgct
      361 tcaagattgc cgatccaact gtgaaggaca cagcccaaaa gccccagcca atggctcttc
      421 gtgagagaat agacaagcgt gtaaatacac actgccacaa gcgcaaacgt caaggcaatc
      481 gccaaattgt agccaaccgc tgcgctgaca ccggccagtt tcgccagacc cgcaaagatc
      541 atatacccgc catagtagta gttgatcgag tgtcctgaga gccaaggatc cgcgggcggc
      601 atccatcggg ttcggaatat ggaactcagc aatgccaaat ccatgtattt ctcggtgcca
      661 tacgtgatgc tcttgtgcat cttgaagaac acagcgacaa ggaaaacgat cagaaacagc
      721 agttcccccc gccaaatgat gggaagatct tcccgaagac gctcgagtaa ctgctcgcgc
      781 tttttcagga gcagaggagc agttgctccc agaatgatca gtgctgcgat cacgattccc
      841 aagcgtgtga aaggcagcag atgaatcgtt gaagcgatcc acacaagata ggttatcaga
      901 agaagcatcg ctggacgggc aaaaagcacc gcccccccac ggattcgaga aaaaacaaga
      961 aagcacaggg gtatccccaa caaacccagg aacgacacgg taaggtacat tttcaacgca
     1021 gtcagaaatt gaagagccat tctttccctc tgattccttt ttctgcttca ggctgcgaca
     1081 gatcgaggat agaatacggt tttcccgctc aggatgtcaa taccagacaa acacataccg
     1141 gcaaacactc tctgtgctgg ctcagttaaa cgggggtgtt tgagacactt gtcattcaga
     1201 atccttcgaa gactcaggac aggctccaca agtcttcgag cggagtgaag aatctctc
//
LOCUS       DAOQUC010001403         1257 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_156314_length_1257_cov_3.054908, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001403 DAOQUC010000000
VERSION     DAOQUC010001403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1257
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_156314_length_1257_cov_3.054908"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1235)
                     /locus_tag="PKH07_18660"
     CDS             complement(<1..1235)
                     /locus_tag="PKH07_18660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNT37019.1"
                     /translation="MTVFTESSDDSSVECWIGQWRERWAEAETSESLAHYRKARLSVA
                     HRIVSPENNYSQFSELVKRGLDVDIAILNAFIGAGSQGNDFELVGPYDVTSRTLKFPI
                     LEKPFCASQDPGKHLQRSRILSNRQFRITTNHAEVMARLKNKETPQDTHHVVLGYGDY
                     RPWQGVIDALHQHAEWVVCIDPNVDERLIVEKASNAQEIREIIGFGSGVGTHGGANYT
                     VSTEQFHFSDVLQKLVCSIAEIYPGWPKGAYPKVAESILQESKRLSGLSLVRATGDGS
                     HIHDFMAYSLTRKMLQGGGDVLCDQTVSLDAYRHWFDSAEDHSRPDLLWLVAKIGDDG
                     RLRLDMRLIECKLAIESVLHLGKAYEQIENGIRHLVPLFMPQIDCSSLEDERPDQRYW
                     WLQLHRLIASRAKIAGAEQ"
BASE COUNT          306 a          350 c          291 g          310 t
ORIGIN      
        1 ttctgctctg ctcctgcaat cttagccctg ctcgcgatga gccggtgcag ctgcagccac
       61 caatagcgct gatcgggtcg ttcgtcctca aggctactgc aatcgatttg aggcataaat
      121 agaggaacta agtgtcggat tccgttctct atctgttcgt acgccttgcc gagatgcaat
      181 acagactcta ttgcgagttt gcattcaatg agtcgcatat ctaagcgcag tcggccgtca
      241 tctccgatct tggcaaccag ccatagcaga tccggccgag aatgatcttc cgcagaatca
      301 aaccagtgcc gataggcgtc cagtgaaaca gtctggtcac agagcacatc accgccaccc
      361 tgaagcatct ttcgtgtaag cgaataagcc ataaagtcat gaatatgact cccatctcct
      421 gttgctcgca ccaaggacag tccggaaaga cgcttggatt cctgcaatat actctcagcg
      481 acttttgggt atgctccctt cggccaacca ggatagattt cagcgatcga gcagacgagt
      541 ttctgcaaga catcagaaaa atggaactgc tccgtggata ctgtatagtt ggctccacca
      601 tgtgttccga cacctgagcc gaagccgata atctcccgaa tctcttgtgc gttactagct
      661 ttctcaacga taagtcgctc atccacattg ggatcaatac agacaaccca ttccgcatgc
      721 tggtgaaggg cgtcaatcac tccttgccac ggtctatagt ctccataccc gagtaccacg
      781 tgatgggtgt cttgaggagt ctctttgttc ttcagacgag ccataacttc tgcatgattc
      841 gtcgtgattc gaaactgccg attgctcagt atgcgcgatc gttgaaggtg cttgccggga
      901 tcttgagaag cacagaaagg cttctccaag atagggaact tgagcgttcg actcgtgaca
      961 tcgtacggac caacgagttc gaagtcattt ccctgactcc cagcaccaat aaacgcattc
     1021 aggatagcaa tgtcaacatc caaaccccgc ttaaccaact cagagaactg actgtagtta
     1081 ttctcaggcg agacgatccg gtgagcaacg gacagacgtg cctttcggta gtgggctagt
     1141 gattctgacg tctctgcctc cgcccatcgc tctctccatt gaccgatcca gcattccaca
     1201 ctgctgtcgt cgctcgactc ggtgaaaact gtcacggcaa gcgcatattc cttaatg
//
LOCUS       DAOQUC010001404         1257 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_156430_length_1257_cov_2.136439, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001404 DAOQUC010000000
VERSION     DAOQUC010001404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1257
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_156430_length_1257_cov_2.136439"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..702)
                     /locus_tag="PKH07_18665"
     CDS             complement(<1..702)
                     /locus_tag="PKH07_18665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37020.1"
                     /translation="MTAPIGLEFLPNVGGEAEGLSDAGIETFRDRPFAAVARETGQNS
                     RDAIDDHSRPVRMAFDIVSIPSSEFCSVDEYRTAARLCLEKARASGNEKEIGFFTQAV
                     AALSSPRIQILRISDHNTRGVRGPCVEGQPFHTLAKADGVSTKEDRNSGGSFGIGKNA
                     AFALSDIQTAFFSTLYRDEGGTNRFLCMGKSLFISHTDGSGTQRRRKGYWGLRQEYMP
                     IEDPSDAPEWARRTEQ"
     gene            complement(699..920)
                     /locus_tag="PKH07_18670"
     CDS             complement(699..920)
                     /locus_tag="PKH07_18670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018858507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37021.1"
                     /translation="MGNKRTKFVSLAEARVTKACKTLRLIGNLSNRQAYEYSEDDVRK
                     IFRTLQRDLEAAKARFTGDGGASSGGFKL"
BASE COUNT          260 a          383 c          312 g          302 t
ORIGIN      
        1 ctgctctgtc ctccgcgccc attcaggggc gtctgaaggg tcttcgatag gcatgtactc
       61 ttggcggagg ccccagtaac ccttccttcg acgctgcgtc cctgatccat ctgtgtggga
      121 gataaaaagc gatttgccca tgcacaagaa tcggttcgtt ccgccctcgt cacggtatag
      181 agttgaaaag aaggccgtct gaatatccga cagagcgaag gctgcgttct tcccgattcc
      241 aaacgaacct cccgaattcc tgtcttcctt ggtgctcacc ccatcggcct tggcaagtgt
      301 atgaaacggc tgtccctcaa cgcagggtcc ccttacgccc ctcgtgttgt gatcggagat
      361 ccgcagaatc tggattctcg gggaggaaag ggcagccacg gcctgcgtga agaacccgat
      421 ctccttctcg tttccgctgg cacgggcctt ctccaaacac agacgggccg ctgtcctata
      481 ctcatcgacc gagcagaact ctgagctcgg gatcgagaca atatcgaaag ccattctgac
      541 tggtcgagag tgatcgtcaa ttgcatcacg gctgttctgg ccggtttccc tggccaccgc
      601 agcaaagggc cgatctctga acgtctcgat gcctgcgtcg ctcaggcctt ctgcctcacc
      661 accaacattt gggagaaact ccaacccgat cggtgctgtc acagtttgaa gcctccactg
      721 gatgcgccac cgtctcctgt gaaccgcgcc tttgctgctt ctaggtccct ctgaagggtc
      781 ctaaagatct tacgcacgtc gtcttccgag tactcgtagg cttggcggtt ggaaagattt
      841 ccaatcaatc gcagtgtctt gcaggccttc gtgacccgag cctcggccag cgacacaaat
      901 tttgtacgct tattgcccat agatgcgccc cttggtgcgc gttattctga tacatttttc
      961 caatattgtc aatatgttga caacgcagct tctgagcatg atggccaaat accacccgag
     1021 acatctcgta aacgcttgtt tatctttgac ttacagtcta ggggtgggag gccaatggtt
     1081 ggcagaccgg agcctgatcc tatttcaaga tccgcagaag ccgctgcttg agtcgaacag
     1141 gctccttgac ttcgcactgc cagatcacgg ctacggtcca ccccatctca accagtgaag
     1201 ccgccgtccg tcgatcacgg tccaagttct tgcggaactt ctcccgccaa aaaccct
//
LOCUS       DAOQUC010001405         1255 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_156713_length_1255_cov_4.019167, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001405 DAOQUC010000000
VERSION     DAOQUC010001405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1255
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_156713_length_1255_cov_4.019167"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(359..>1255)
                     /locus_tag="PKH07_18675"
     CDS             complement(359..>1255)
                     /locus_tag="PKH07_18675"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNT37022.1"
                     /translation="DPHRPFAGSRGEEAQEQKRGRQFPAPAQVYRPEEVTVPGFLPDI
                     PGVREEMSHYYTSARRCDETLGAVIRALDEAGVSEDTCVTFLSDNGISQPFAKTNCYL
                     SSTRTPWLVRWPGQVPMGVVDHEHMISGIDYASTVLEAANLEPMQGVDGHSFLPVLRG
                     KSQQGRDLLLTCMNQTSAKRDYPMRCLMTRRFGYIFNAWSDGKTEFQNEPCGGMAFAA
                     MREAAETDEAIAARVRFFKLRVPEEFYDFQKDPCALNNLIDEPALSEEISTLRRRLHQ
                     IMRTTQDPLADIFETRVVFGNR"
BASE COUNT          304 a          354 c          291 g          306 t
ORIGIN      
        1 ctccttcgca atattgctgc tccagccaga atgtactggc agcactgtga gcagcccaag
       61 aaccatcaag cccacgatcc aagacctctg ccctgttcta cacatcgttc gatccatctt
      121 catatcctcc ttgctcatct accatgcgat ggataacgaa accccagact gaaagacaat
      181 ccctggcgtg tactcaccaa gaccagatat cgcctcagtc ctccaacatg cctcctgaac
      241 aaacctacat aggaaaaaca aacactacca ccactccgcc aacagcgaat atgtctcacc
      301 gcatatacgt tgtgttatag ttgattttag cttttgtgtc aatagcgaac ggaaattcct
      361 aacggtttcc aaacacgact ctggtttcaa agatatcggc taaggggtct tgtgttgttc
      421 gcatgatctg atgaagccgt cttctcagtg tgctgatttc ttccgaaagc gccggttcgt
      481 ctatcaggtt gttcaaggca cacgggtcct tctggaagtc gtagaattcc tctggaaccc
      541 gcagtttgaa gaatctgacc cgagctgcta tcgcttcatc agtctctgcg gcctccctca
      601 tggcagcgaa ggccattcca ccgcaaggtt cattttgaaa ctctgtcttg ccatccgacc
      661 aagcgttgaa tatgtagcca aaacgtctgg tcatcaagca acgcatcggg tagtctcgtt
      721 ttgcagaggt ctggttcata catgttaaga gcaaatcccg accctgttga gattttcctc
      781 tgagaactgg aagaaaggag tgtccatcaa ctccctgcat gggttccaaa ttggccgctt
      841 cgagaacggt cgaagcatag tcaatgcccg aaatcatgtg ttcgtggtct acaacgccca
      901 tgggaacttg tcctggccag cgcaccagcc acggggttcg agtgctcgag agataacagt
      961 tcgttttggc aaaaggctgc gagatgccgt tgtcactcaa gaaagtcaca caggtatcct
     1021 ccgaaacgcc tgcttcatcc aaggcacgga tcaccgcgcc gagcgtctcg tcacatcgtc
     1081 gggctgatgt gtaataatgt gacatctcct cacgaacacc ggggatgtcg ggaaggaatc
     1141 ctggaacagt gacctcttct ggacgataga cctgagctgg ggcagggaac tgtcgccccc
     1201 ttttctgttc ctgtgcctct tcgccacggc tgcctgcaaa aggccgatgc ggatc
//
LOCUS       DAOQUC010001406         1255 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_156807_length_1255_cov_2.332500, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001406 DAOQUC010000000
VERSION     DAOQUC010001406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1255
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_156807_length_1255_cov_2.332500"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1255
                     /locus_tag="PKH07_18680"
     CDS             <1..>1255
                     /locus_tag="PKH07_18680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37023.1"
                     /translation="LFVVAVMGVSAFESREKDGTTFFLYQVPVSPIRMFCAKYLTGLY
                     LTVSSILCITVGLIMLKGTSFLTNVMLPETDVAVPIIWCVYTIAFLVSPMIASDISAL
                     GATSIGAALASCLFFSLTLKHPALVTLEETQFWKGSYWRIIFATDLLVTLMILCVAML
                     LFRRFRCLRLSFRQRAVVLMLLCTAASLWIFCNVFIDFHDLFYLLTTRTKVYVADMET
                     QREILHALAEKVDRYREERYGPSCYDKFLHIFGDYESRKKSPKYPIRKRHIYGTRRSI
                     EEMQWLYSKNTLEQQRIQEYLDGRFHWWEKLSEALSCGAMYQRTVEIVENPNSACLLG
                     LSDVQDCVRDLCTKVLYEYAHGQGDRGLSALGLALRLASECRVDSSSLGESKFFRAVM
                     RNCIEDILWRNPEAEWTATASRQTEA"
BASE COUNT          272 a          307 c          333 g          343 t
ORIGIN      
        1 gtctgtttgt cgttgccgtc atgggagtct cggcgttcga gagtcgtgag aaagacggca
       61 cgacgttctt cctctaccag gttcccgttt caccgataag gatgttttgt gcaaagtatc
      121 tcaccgggct gtatctcaca gtcagttcga ttctgtgcat caccgttggc ttgatcatgc
      181 tcaagggaac gagttttctg acgaacgtga tgctcccgga gacggatgtt gcagttccaa
      241 tcatctggtg cgtttacacc attgcgttcc tggtttcgcc catgatcgca tcggatatca
      301 gcgccttggg tgcaacttcc atcggagcgg cgcttgcctc ctgcttgttc ttcagtctga
      361 ctctgaaaca tcctgctctt gtcaccctcg aagagacgca attctggaaa ggttcgtact
      421 ggcggataat cttcgccacg gatctactcg tcacgctgat gattctatgc gtggcaatgc
      481 tcctctttcg acgttttcga tgcctgcggc tcagtttccg gcaacgcgca gttgttctca
      541 tgctgctgtg cactgcggct tcattgtgga ttttctgcaa cgtcttcatt gatttccacg
      601 accttttcta tctactgacc acccgaacga aagtgtatgt tgcggatatg gaaacacagc
      661 gtgagatact tcacgccctc gctgagaagg tggaccggta cagggaggag cgttacggac
      721 cttcatgcta tgacaaattc ttgcatattt tcggggatta cgagtcaagg aagaagagcc
      781 cgaaataccc gattcgaaaa cgacacatct atgggacgcg ccgtagtatc gaagaaatgc
      841 agtggttata cagcaagaac acgttggagc aacagagaat acaagagtat cttgatggtc
      901 gtttccattg gtgggagaaa ctgtcagaag cgttgtcgtg tggtgcaatg taccaacgca
      961 ccgtcgagat tgttgagaat cccaatagtg cctgcttgct tggactgtcc gacgtacaag
     1021 attgcgttcg tgatctctgc actaaggtac tctacgaata tgcgcatggt cagggtgatc
     1081 gtggtttgag tgcccttggt ttggcgctta gattggcctc cgagtgccgt gtagactcat
     1141 cctctctcgg agagagcaag ttttttcgag cggtcatgcg caactgcatt gaagatatcc
     1201 tatggcgcaa tcctgaggct gagtggactg cgactgcatc tcgacaaacc gaggc
//
LOCUS       DAOQUC010001407         1252 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_157424_length_1252_cov_2.431078, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001407 DAOQUC010000000
VERSION     DAOQUC010001407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1252
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_157424_length_1252_cov_2.431078"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..762)
                     /locus_tag="PKH07_18685"
     CDS             complement(<1..762)
                     /locus_tag="PKH07_18685"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNT37024.1"
                     /translation="MWRPFAAILVIVFCILLLGCWLWAPLNQTVSLRVPIPENNPDLI
                     LAGSSGDIPGRLASGIGVPSSGDTGAWPQFRGAHRDGVATGSPIREGFPEDGPEVLWR
                     ISVGEGHAGAAIHSGCVYLLDYDEQAREDVVRRLALWNGEDIWRYSYPVRIKRNHGMS
                     RTVPAVNDEYVVTIGPMGHVHCLKAASGELVWSKDLTRDYGTRIPEWYAGQCPLIEPD
                     RVILAPVGKKYLMTAIDLATEEAIWETPNDLGWEMT"
     gene            complement(853..>1252)
                     /locus_tag="PKH07_18690"
     CDS             complement(853..>1252)
                     /locus_tag="PKH07_18690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37025.1"
                     /translation="LSFFGMPFGLSVRDAELGWNWTYWRAVLKPLFAEIKPGSELYPV
                     LTAPDSSYPLRFTGAPQIEVMCRESGVYLYIFAAAREGKSKRVQFSGVEDGDVTVLFE
                     NRTLQASGGRFADRFDEHDVHIYRATRKGH"
BASE COUNT          318 a          366 c          300 g          268 t
ORIGIN      
        1 tgtcatctcc caaccgagat cattcggcgt ttcccaaatg gcctcttcgg tagcaagatc
       61 gatggcagtc atcagatatt tcttccccac tggagccaga atcacccgat ccggctcaat
      121 gagcggacac tgaccggcat accactcagg aatcctggtt ccatagtccc tggtcagatc
      181 tttgctccaa accagttcac cactggcagc cttgaggcag tgaacatgac ccatggggcc
      241 aattgtcacc acgtactcat cgttgactgc gggcacagtt cgggacatgc catggttgcg
      301 cttgatcctg actggatagg aatatcgcca aatgtcctcg ccattccaga gtgccagacg
      361 acgcacaacg tcttctctcg cctgctcatc ataatcgaga agatagacac acccgctgtg
      421 gatggcggct ccagcatgtc cttctccaac ggaaatccgc cagagtactt caggcccatc
      481 ctcagggaac ccttcacgaa tcggcgaacc ggtagcgaca ccgtcacgat gtgcaccccg
      541 aaactgtggc cacgcaccgg tatctcctga ggaaggaact ccgatccctg atgcaagtct
      601 gccgggaatg tctcccgatg agccagccaa aatgagatca ggattgttct cgggaattgg
      661 aacccgcagg gaaacagtct gattcaatgg agcccaaagc cagcaaccca ataagagtat
      721 gcagaagaca atgacgagta ttgctgcaaa aggtcgccac atgactccat tgctccgatc
      781 atcatacgaa gacttgtttc tcagaacgtt tggagagata cctcattaac gcattgatca
      841 ggcggccagg tcttagtggc cttttcgggt cgcccgatag atatgaacat catgctcatc
      901 gaatcgatcg gcaaatctgc ctcctgatgc ctgaagagtt cggttctcaa aaagcacggt
      961 gacgtcccca tcttcaacac ccgagaattg gacgcgtttc gacttccctt ctctggccgc
     1021 tgcaaagatg tagagataaa ctcctgactc ccgacacatc acctcgatct gcggcgcacc
     1081 ggtaaaacgc aacgggtaac tcgaatccgg cgctgtcagg acgggataga gttcactgcc
     1141 tggtttgatc tctgcaaaga gtggtttcaa gacagctctc caatatgtcc aattccagcc
     1201 cagttccgcg tcgcgcacag acagaccgaa gggcatcccg aaaaatgaaa gg
//
LOCUS       DAOQUC010001408         1252 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_157495_length_1252_cov_1.432749, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001408 DAOQUC010000000
VERSION     DAOQUC010001408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1252
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_157495_length_1252_cov_1.432749"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..938)
                     /locus_tag="PKH07_18695"
     CDS             complement(<1..938)
                     /locus_tag="PKH07_18695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37026.1"
                     /translation="MEPPPPECRGLVSESGGDPNPFVAYLNSLHSCSAASENALAESQ
                     CCNPFFSFIHVAHPLAETIRETLLDSPRRHVILTGHAGDGKSTIAVEVFKTLSGKSFS
                     EPLSKPLSRREDLSAEGREITLIKDFSEWSEEERSALMTEMLDASSPRFLLISNTGTL
                     LDAFKRYESRVGQDWVKIESDLLKSMDTSRSAPIEFHDTSFALINVAVFDNLVMAESI
                     LRRMLDAERWSACASVECRQCCPMYQNVLLMQRNLSVVCERMFLAYRRMYEYGTRLTL
                     RQLTAHMAYMITSGLQYSDVAKMFQKASRPCMSEFM"
     gene            999..>1252
                     /locus_tag="PKH07_18700"
     CDS             999..>1252
                     /locus_tag="PKH07_18700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37027.1"
                     /translation="MIIDSLERLAHLRFELRDLLLELLAGEAGDHLSLRDGLALQDFA
                     ADKVRGSVRPTSSPWSGWMATRPVSRTRLIGSGSRKNDAI"
BASE COUNT          304 a          353 c          315 g          280 t
ORIGIN      
        1 aacatgaact cggacatgca gggacgactc gccttctgaa acatctttgc aacatcagag
       61 tactgaagac ctgaagtgat catgtacgcc atgtgcgccg tcaactgacg caacgtgagt
      121 cgagtcccgt attcatacat ccttcgatac gcgagaaaca ttctctcgca gacaacagat
      181 aggttccttt gcatcagcaa gacgttttgg tacataggac agcactgccg gcattcaacc
      241 gaagcacatg cagaccatcg ttccgcgtca agcatccgtc gaagaatgct ctcagccatg
      301 acgagattgt caaataccgc aacgttaatc agtgcgaacg aggtatcgtg aaactcaatg
      361 ggggctgatc ttgaagtgtc cattgactta agcagatcgc tttcaatctt cacccagtcc
      421 tgcccaacac gtgactcgta gcgcttgaat gcatcaagca gagttcccgt gtttgagatc
      481 agcagaaaac gcggagaact cgcatcaagc atctcagtca tcaaagcgct acgttcttcc
      541 tcagaccact cactgaaatc cttgatcaaa gtgatctctc tgccctcagc agacaaatcc
      601 tctcttctgc tcaatggttt ggataagggt tcgctgaacg atttgcctga aagggtcttg
      661 aatacttcaa cggctatcgt tgactttcca tctcctgcgt gaccggtcaa aatcacatgc
      721 cgcctggggg agtccaagag tgtctccctt atcgtctctg ccaacggatg cgcaacatgg
      781 atgaagctga aaaacggatt acagcactgc gactcggcta gcgcattctc gcttgcagca
      841 ctacaactat gcagtgagtt aaggtaggca acaaaaggat tcggatcacc gccagattca
      901 gagacaagac cacggcattc cggcggaggc ggttccatct cagacgattc cgggagggag
      961 gaaactatct cgggttcctt cgttggaact ccctttcctt gatcatagac tctctggagc
     1021 gcctcgcgca tctccgattc gagcttcgtg atctcctcct ggagcttctg gctggcgaag
     1081 cgggcgatca cctgtccctt cgtgacggtc tggccctcca ggacttcgcg gcggacaagg
     1141 tgcgcggttc cgtgaggccc acgagctcgc cgtggtccgg gtggatggcc acgagacccg
     1201 tctcccgcac gcggctgatc ggcagcggca gcaggaagaa cgacgcgatc aa
//
LOCUS       DAOQUC010001409         1251 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_157529_length_1251_cov_3.413043, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001409 DAOQUC010000000
VERSION     DAOQUC010001409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1251
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_157529_length_1251_cov_3.413043"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..841
                     /locus_tag="PKH07_18705"
     CDS             <1..841
                     /locus_tag="PKH07_18705"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007290067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004365 - glyceraldehyde-3-phosphate
                     dehydrogenase (NAD+) (phosphorylating) activity [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HNT37028.1"
                     /translation="AGDGVIKVDGKEIKVLAIKEGPGAMPWGDLGVEYVVESTGIFTD
                     GEKANLHITKSGAKKVIISAPAKGGDVLTVVMGVNEDAYDPKKHNLLSNASCTTNCLA
                     PVVKILLDNFGIASGLMTTIHAYTNDQKILDLPHKDLRRARAAAMSMIPTTTGAAKAI
                     GEVIPQLKGKLHGYAMRVPCPNVSVVDLTVNLEKDATAEAINAAVKAASEKAPLKGYL
                     RYVDEPLVSIDFNGCAASSIFDAGQTMVLQSRFAKVLSWYDNETGYATRVVDLIQYMV
                     GKG"
     gene            929..>1251
                     /gene="pgk"
                     /locus_tag="PKH07_18710"
     CDS             929..>1251
                     /gene="pgk"
                     /locus_tag="PKH07_18710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HNT37029.1"
                     /translation="MKKKTIRDIDVQGKRVLMRVDFNVPLAEGKVANDQRISQALPTI
                     KYLLDKDARLVLMSHLGRPKGKVAEEFRMAPAAKRLEELLGKPVKTVSDCIGTEVEKA
                     AADLKP"
BASE COUNT          306 a          338 c          352 g          255 t
ORIGIN      
        1 agcaggcgat ggcgtgatta aggttgatgg caaggagatt aaggttctcg ccattaagga
       61 aggccctggc gcgatgccgt ggggcgatct gggtgtcgag tacgtcgtcg agtccaccgg
      121 tatcttcacc gatggtgaga aagccaacct gcatatcacc aagtcgggcg caaagaaagt
      181 cattatctcc gcgccggcca agggtggcga tgtcctgacc gtagtcatgg gcgtaaacga
      241 agatgcctat gatcccaaga agcacaacct gctctccaat gcatcctgca cgacgaactg
      301 tctcgctcca gtcgtaaaga tacttctgga caatttcgga attgcttccg gcttgatgac
      361 gacaattcac gcgtacacca acgatcagaa gattctggat ctgcctcaca aggatctgcg
      421 tcgcgctcgc gcggctgcga tgagcatgat tccgaccacc acaggcgctg ccaaggccat
      481 tggcgaggtc atcccgcagt tgaaaggcaa actgcatggc tacgcgatgc gtgtgccctg
      541 cccgaacgtc tctgtggttg atctgactgt gaacctggaa aaggacgcaa ccgcagaagc
      601 catcaatgcc gctgtgaagg ccgcttctga gaaagcccct ctgaagggtt acttgcgtta
      661 tgtggatgag ccacttgttt ccattgactt caatggatgt gccgcatcct ccattttcga
      721 cgcaggtcag actatggtgc tgcagtcccg cttcgccaag gttctctcct ggtacgacaa
      781 cgaaaccggt tatgccacgc gcgtggttga cctgattcag tacatggttg gcaaaggcta
      841 gcctttgtgg caacgagaat gaagccacgg ggggctctcg caaacccgag agccctccgt
      901 gaatctccag tgaaccgagg aagcaaagat gaagaagaaa acaattcgag atattgacgt
      961 gcagggcaag cgcgtcctca tgcgtgtgga tttcaacgtt cccctggcgg aggggaaagt
     1021 ggcgaacgat cagcgcattt cccaggcact gcccaccata aagtaccttc tggacaaaga
     1081 cgctcgtctg gttctgatgt cccacttggg aagacccaaa ggcaaggtcg ccgaagaatt
     1141 ccggatggca cctgcggcca agcgcctaga ggaacttctg ggcaaacccg tcaaaacggt
     1201 ctccgactgc atcggaacag aagtggaaaa agccgctgcg gatctaaagc c
//
LOCUS       DAOQUC010001410         1251 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_157552_length_1251_cov_2.856187, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001410 DAOQUC010000000
VERSION     DAOQUC010001410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1251
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_157552_length_1251_cov_2.856187"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1144)
                     /locus_tag="PKH07_18715"
     CDS             complement(<1..1144)
                     /locus_tag="PKH07_18715"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNT37030.1"
                     /translation="MSSEGRASGKHLLVLLLLCLSVWGWTYGLWDLWGADETRYTQVS
                     RELLTRNNWLVLTVFDRPYDQKPPLSFWIYALMLKLAGGRISVWHLRLAPILFAMATL
                     GMTYFLGRRVCGEKVGFLASLMVFTSTVFISEATEVKMDIMFTAWITLSLTLWLCRTK
                     GEPLGLLRAFGVWAALAAAFFTKGPLAILIVISSIATEAWSEHTWKVFRDARTVWGLL
                     GLGLLIAGWLHAQASAAGSEFVATQVRGETLNRLLHGAHDEPVWFYFNSLYQKIFTPW
                     VLLWIPLFLRLRSGQMRFPKQLLPFAGWFAIPFLILIIARGKRPPYMLPLLPAMAIVT
                     AWLLDRLIEERRFAPKLNRALCILFPILGVALLIAVIILQRFTQLHV"
BASE COUNT          350 a          337 c          332 g          232 t
ORIGIN      
        1 gaacatgcag ttgagtgaac cgctgaagga tgatgactgc aatgagcaga gcgacgccca
       61 agatcggaaa caagatgcac aaggctcggt tcagcttcgg agcgaatctt ctctcttcaa
      121 tgagtctgtc aagaagccat gcagtcacga tagccattgc cggaagcaac ggaagcatgt
      181 acgggggtct cttgcctcgc gcgatgatca gtatcagaaa tgggatggca aaccagccag
      241 cgaaaggaag aagctgcttg ggaaatctca tctgccctga gcgaagacgc agaaagagcg
      301 gaatccagag taagacccat ggagtgaata tcttctgata gaggctgttg aaatagaacc
      361 agactggctc atcatgagca ccgtgtagca gtcggttgag cgtttcgccg cgaacctgcg
      421 ttgccacaaa ctccgagccg gcagccgatg cctgagcgtg caaccaacct gcgatcaaca
      481 agcccaagcc gagaagaccc cacacagtgc gggcgtcacg aaagactttc catgtgtgtt
      541 ccgaccaggc ctctgttgcg atggaagaga taacgatcag aattgccaag ggacccttgg
      601 tgaagaaggc agcagccaac gctgcccaga ctccaaatgc acgaagaaga cccagaggct
      661 ccccttttgt gcggcaaagc cacaaggtca gcgaaagagt gatccaagcg gtgaacatga
      721 tgtccatctt cacctctgtg gcctcgctga tgaagactgt cgaagtgaag accatcagag
      781 aagcaaggaa ccccactttc tcaccgcaga cccttcttcc cagaaaatag gtcatgccga
      841 gcgttgccat ggcaaacagg attggtgcca ggcggagatg ccaaacggaa atccgccccc
      901 cagcgagttt gagcatgagc gcatagatcc aaaacgacag aggcggcttc tgatcataag
      961 ggcgatcgaa aacagtgaga acgagccagt tgtttcgggt cagcagttca cgagacacct
     1021 gcgtatagcg agtttcgtct gccccccaca aatcccagag accgtatgtc cagccccaga
     1081 ccgaaagaca aagaagcagc aacacaagaa ggtgtttccc ggatgcacga ccttcactgc
     1141 tcacctgagg acaccccatc cttaacatat tcagcgcttc ggcatgtgca tgaacttcga
     1201 cgttttcgct ctttcgttgc actagattaa gagtaggacg ctttctccag g
//
LOCUS       DAOQUC010001411         1250 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_157828_length_1250_cov_2.348954, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001411 DAOQUC010000000
VERSION     DAOQUC010001411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1250
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_157828_length_1250_cov_2.348954"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1250)
                     /locus_tag="PKH07_18720"
     CDS             complement(<1..>1250)
                     /locus_tag="PKH07_18720"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNT37031.1"
                     /translation="LAFNECHRWAAFGMILLTAVMFWHALTCDVGFGFFPEPDRGEFY
                     LKLEYPTDYDLQRTEQLVRQVEERLSDLPHLRHMLTTCGRVQGVVGQSSEGVYLAQLL
                     LRFNERTEREMTAFDLLSEIRRRVSDLPGQIATVTVASIIGGQSTPIEMEISGEDLTL
                     LDRLAERSAELAKGTGTMVDVDTTVRAGKPELRVLPKREVLSDLQTPARALGYTLRGN
                     IEGLNAGSFTSGDRNYDIVVKFAEKPGLQQVAAFELPGKAGRPIRLDTVARIESGVAP
                     IQIIRKNKMRIAKLYASLKEGVPLGTAVNSLSAAMDERGNLPPGYDYYFAGEYEVMHE
                     ANVGFLEALLLSILLVVLTLAAILESFRQPWLILVTLPLGLIGVLWSLALMGLSMDMF
                     AVMGLVMLIGIVVNNAILIMDQFN"
BASE COUNT          318 a          361 c          311 g          260 t
ORIGIN      
        1 cgttgaactg atccatgatg aggatggcat tgttgaccac aatgccaata agcatgacaa
       61 gacccatcac agcaaacatg tccatggaga gtcccatcaa tgccaaagac cacagaacgc
      121 cgatcaaccc aagtggcaag gtcacaagga ttagccacgg ctggcgaaag gactccagaa
      181 ttgctgcaag cgtgagcacc accagcagaa tggaaagcaa gagtgcctca agaaaaccaa
      241 cgtttgcctc atgcatgacc tcgtactcac ctgcaaaata gtagtcatat cccggtggaa
      301 gatttccccg ctcatccatc gctgccgaaa ggctgttgac tgcagtgccc agaggcacac
      361 cttccttcag actggcatag agtttggcaa tccgcatctt attcttgcga atgatctgta
      421 ttggagccac acccgactca atgcgggcga ctgtgtccag gcgaatcgga cgccctgcct
      481 tcccaggcag ttcaaatgcc gcgacctgtt gaagaccagg tttctcagcg aacttgacga
      541 caatgtcgta gttgcgatcc ccactagtga agcttccggc attcagacct tcaatgtttc
      601 ctcgaagggt atatcccagc gcacgagcgg gagtctgaag atcactgaga acttcacgtt
      661 tgggaagcac acgcagttcc ggcttgcctg ctctcaccgt cgtgtcaacg tccaccatgg
      721 ttccagtgcc ttttgccagt tcggcacttc gttctgcaag gcgatccagg agggtcaaat
      781 cctctcccga tatctccatc tcaatcggcg tgctctgacc accgatgata gaggcaactg
      841 taaccgtggc gatctgcccg ggtaagtctg agactcgacg ccgaatctcg ctgagcagat
      901 caaatgccgt catctctcgt tcagttctct cgttgaagcg caagagcaac tgagcaaggt
      961 agaccccttc ggaggactgt cccacgacgc cttggacgcg cccgcaagta gtcagcatgt
     1021 gacgtagatg gggcaggtcg ctcaaacgct cctcaacttg tcgaaccagc tgctccgtgc
     1081 gctgcagatc atagtctgtc ggatactcaa gcttgaggta gaactctcct cggtctggct
     1141 ccgggaagaa accgaaacca acatcacaag tgagcgcatg ccagaacatg actgccgtca
     1201 gaagaatcat cccaaaggca gcccaacgat gacattcgtt gaaggcgaga
//
LOCUS       DAOQUC010001412         1249 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_158027_length_1249_cov_2.306533, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001412 DAOQUC010000000
VERSION     DAOQUC010001412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1249
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_158027_length_1249_cov_2.306533"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..467)
                     /locus_tag="PKH07_18725"
     CDS             complement(<1..467)
                     /locus_tag="PKH07_18725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37032.1"
                     /translation="MVSISTEACPRVCQGDIVRDVEYFERVTEQEGIIELSKVVFPLA
                     IVLTQDCDLAQDHLFRTEEQATQDKWLISVLLAPIYNAEHVFAGEHLSEIGVKSQLIR
                     KNKTDGQRVVQNKDPRYHYLEFPGGVEVVPSIVDFKHYFACPVAYLETVKTNGG"
     gene            complement(620..>1249)
                     /locus_tag="PKH07_18730"
     CDS             complement(620..>1249)
                     /locus_tag="PKH07_18730"
                     /inference="COORDINATES: protein motif:HMM:TIGR04255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04255 family protein"
                     /protein_id="HNT37033.1"
                     /translation="PIEEPKPLVTQEMRLVGKEVKTTMKEMTEWNFHGRLRDKRLVIA
                     PKAFLISYTKYLQYEMLQKEFQAIADVFFSCIGNAQASRLGLRYVNELKIPGENVLDW
                     HDYVDARLLGMCTYSVEEAEPSRIFNIYEVVYPAQGFNMRFQFGLYNPDYPASIRRRV
                     FVLDYDAYFQGPIDPEDIASALDRYHRAIQSLFERSITEKTREALNERE"
BASE COUNT          291 a          356 c          279 g          323 t
ORIGIN      
        1 ccaccgttcg ttttcacggt ttccagataa gccacaggac aggcgaagta atgcttgaag
       61 tcaactattg atgggacaac ctccacccct cccgggaatt ccaggtagtg atatctcggg
      121 tctttgttct gcaccacacg ctgcccatcc gtcttgtttt tcctgatgag ctgtgatttt
      181 accccaatct cggacagatg ctccccagca aaaacatgtt ccgcattgta tatcggcgca
      241 agcagtacag agataagcca cttgtcctga gtcgcctgtt cctctgtacg aaacagatga
      301 tcttgtgcga ggtcgcaatc ctgcgtaaga acaatcgcca acggaaagac cactttagac
      361 agctcaatga tcccctcttg ctctgtgacc ctctcaaagt actcgacatc ccgtacgatg
      421 tcgccttggc aaactcgcgg gcacgcttcc gtactaatgc tcaccatgtg ttatcctcgc
      481 acgtatgata acccttcgcg gcttcccgct ttcatatcga gcatccacct gttcctgttc
      541 agggtattca ggggttggtt gtaacatctg aacacgcggc atagaggagg tgtcagtgcc
      601 gccttcactc gtgatctcat cactcgcgtt cattgagcgc ctcacgcgtc ttctcggtaa
      661 tgctgcgttc aaagagggac tgaatcgccc tgtggtacct gtctaaggca ctcgcgatgt
      721 cttccggatc gataggtcct tgaaaatagg catcatagtc caacacaaat accctccggc
      781 ggattgacgc cgggtagtcc ggattataga gtccgaactg aaagcgcata ttgaaccctt
      841 gagctggata cacaacctcg tagatgttaa atatacgcga cggttcagct tcctccacgc
      901 tgtacgtaca catccctagc agtctggcat caacatagtc gtgccaatca agtacattct
      961 cgccgggtat cttgagttcg ttgacgtagc gaagccccaa tctgcttgcc tgcgcgtttc
     1021 caatgcacga gaaaaacaca tctgcaattg cctgaaattc cttttgcagc atctcatact
     1081 gaagatactt cgtataggaa atgaggaacg ctttcggtgc aatcaccaga cgcttgtcgc
     1141 gaagccgccc atgaaagttc cactccgtca tctccttcat tgtggtcttg acttccttgc
     1201 cgacaagacg catctcctgc gtcacaagag gtttcggctc ttcaatagg
//
LOCUS       DAOQUC010001413         1248 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_158196_length_1248_cov_2.776194, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001413 DAOQUC010000000
VERSION     DAOQUC010001413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1248
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_158196_length_1248_cov_2.776194"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1010)
                     /locus_tag="PKH07_18735"
     CDS             complement(<1..1010)
                     /locus_tag="PKH07_18735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37034.1"
                     /translation="MRNSDETSHPPCQDMELAKIANELLGDHCNVPSDSSSQHAESAR
                     FDIEKSVGSVSTNHPDISNPYAQVRTVRRDIERPRVGCVKAEQLTDYHAACVFRLAGV
                     GPQASGSDTGPDPVCNPAFLARFLCSSHISNPIELRYVFFPRTKESPQRQLSTYLITH
                     VLDGLQEELPVRMNATVSTMASLLSTTRSGYDFEPVTDKVLYDHGLTPFPIQHIAEFL
                     RHEELIGVKGGGTRYCALPFFFARGGIGHLADLLVSHTHPLLLSMQIWTRHISEQTLE
                     YLRDSIVLVEGSDEDASLRFESNLMGGSSLDTSPQPVQQHLAAESYRALGFGRRLYIS
                     RIL"
BASE COUNT          290 a          325 c          341 g          292 t
ORIGIN      
        1 aggatcctgc tgatgtagag tcgtctgccg aaaccaagtg cgcgatagga ttcggctgcc
       61 aagtgctgct gaacgggttg aggggaggtg tccaaagacg atcctcccat cagattggac
      121 tcaaatcgca aagaagcatc ctcatcggaa ccctcaacga gaacaattga gtcccgcaaa
      181 tactccaggg tctgctctga aatgtggcgc gtccagatct gcatggacag aagcaaggga
      241 tgcgtgtgag aaaccagaag atcagcgagg tgaccgatcc cgcctctggc aaagaagaaa
      301 ggaagtgcgc agtatcgagt ccctccgccc ttaacgccaa tgagttcctc atgtcggaga
      361 aactctgcta tgtgttgaat cgggaagggg gtcaggccat ggtcatagag aaccttgtcc
      421 gtgacaggtt cgaaatcata acctgaacgg gtcgtactca ggagtgaagc catcgtactg
      481 accgttgcgt tcattctcac ggggagttcc tcttgcagac cgtccagaac gtgagttatc
      541 aggtaagtgc tcagttgtcg ctggggactc tccttggtgc gtgggaagaa gacgtaacga
      601 agttcgatgg gattggagat atgcgagctg caaagaaatc ttgcgagaaa ggcaggattg
      661 cagactggat ctggacccgt atcactgccg gaagcctggg gacctacccc ggcaagacgg
      721 aacacacagg cagcatggta gtcggtaagt tgctcagcct tgacgcaacc cactcgcggt
      781 ctttcaatat ctctacgaac cgttctgacc tgggcgtatg gattggagat gtctgggtgg
      841 ttggtggaga cagagccaac actcttttcg atatcgaacc tcgccgactc agcgtgttga
      901 gaggaggagt cggaaggaac gttgcagtga tcccccaaca actcattggc aatctttgcc
      961 aactccatgt cttggcaggg cggatgggaa gtctcgtcgc tattcctcat cgtctggctc
     1021 ctttctcaag gcactctctc tagtccttct gaatccctct cttggtatga ggcagattgg
     1081 ctccatgaac gggagtattc tgttgccagg atgtcccggt atccccgaat ccgattcagt
     1141 tgttcaaagg ctgcttctta cttctggata gcccccccct ctctaccaac tgcagatatc
     1201 tcttcccgaa tcctcttctc ggcattcctg cgatagcctc gccttcct
//
LOCUS       DAOQUC010001414         1246 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_158629_length_1246_cov_2.992443, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001414 DAOQUC010000000
VERSION     DAOQUC010001414.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_158629_length_1246_cov_2.992443"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            292..>1246
                     /locus_tag="PKH07_18740"
     CDS             292..>1246
                     /locus_tag="PKH07_18740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37035.1"
                     /translation="MSLRTRVRRFTESALELARMDSVDQMCCRAVELARSDLGFERCG
                     LFLRSRNDPNLFEGTYGTNAQGEIRDEHHLQYDIRNDEKLAEIVENRQKYPILSKGIS
                     CGDGEGIEKTADHAAHLIWGGNQNLGIIFTDTLLTGRSLTEEDMDMLMLYSSTVGHLI
                     GTLQTHESLRTQNRQLQAFRAVAETIVSSSSTEDLAERLSEIIRSLLPCDTFFLNLYD
                     PVVDSIIPVYHWNRSTDVPESVKGMMFSAALTSAIRSLLTTRQPLVLFRSEDHQDFLQ
                     ESIPSYSSQIYLPLLSAAKAAGYFSVQTDTPQAYSVEDINML"
BASE COUNT          297 a          321 c          327 g          301 t
ORIGIN      
        1 aaccgagagg ggagaacgtg tgtcaatatg aggacgtccg gatatgcgct catcgccctg
       61 ctgaaggccg aaagcagcct gcgcaacatc tggttggggc tgcacaacga tcccttcaga
      121 gatcagcgct ggatcgcacc gcataatctg atctggtaag gcgttttcat gaagaacggg
      181 ccaaaaacga gaacagaact tgtcgaggag aaccagcgtc tgcgtgctga gtgcgaccgg
      241 ttgcgagacg gttcttgttc gtctcatgaa ccggctgagg aagagcagcg catgtctttg
      301 cggacaagag tccgtcgctt cacggagtct gccctggaac ttgctcgcat ggattcggtt
      361 gatcagatgt gctgcagggc tgttgaactt gcccgatccg atctgggttt tgagcgctgt
      421 ggcctgttct tgcgaagcag aaatgatccg aacctctttg aaggcaccta tggaactaat
      481 gcccagggag agattagaga cgaacaccat ctgcagtacg acatccggaa cgatgagaaa
      541 cttgccgaga tcgttgagaa ccgacagaag tatcccattc tctcaaaagg aatctcgtgt
      601 ggtgatgggg aaggtatcga aaagaccgct gatcatgccg cgcatctcat ctggggagga
      661 aatcagaatc tgggtattat cttcactgac accctgctga ccggacggtc tctgactgaa
      721 gaagatatgg atatgcttat gctctattct tcgaccgtcg gtcatctcat cggaacgctg
      781 caaacccatg aatcactgag aacgcagaat cgtcaactgc aggctttccg tgcggttgcc
      841 gagacgatag tctcctcatc ttccacggaa gatctcgccg agagactgtc tgagatcatc
      901 agaagtctgc ttccttgtga cactttcttc ctgaatctct acgaccctgt ggttgactca
      961 ataattcctg tctaccattg gaatcgctca accgatgttc ctgaatcggt caaggggatg
     1021 atgttttcgg ctgccctgac aagcgcgatc agaagcctgc tcactacgcg acagcccctt
     1081 gtccttttcc gcagtgagga tcaccaggac tttttgcagg agtctattcc ctcttactct
     1141 tcccaaatct atcttccgct gctgtcggca gcaaaggcag caggttactt ctcggttcag
     1201 acggacactc ctcaagcata cagtgttgag gatatcaaca tgcttg
//
LOCUS       DAOQUC010001415         1244 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_159073_length_1244_cov_2.846930, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001415 DAOQUC010000000
VERSION     DAOQUC010001415.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1244
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_159073_length_1244_cov_2.846930"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1244)
                     /locus_tag="PKH07_18745"
     CDS             complement(<1..>1244)
                     /locus_tag="PKH07_18745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37036.1"
                     /translation="IQTWNANNDNPKFILATPEEFFEYLVANYDSQIPEHSGNWTSLW
                     DGGSMTEPKSEKIVKNAQDEVLATEKMWAIASEVGLGTYPHDEFTNAWDMMLTVDEHS
                     GGGGGWADYFTQEEVDANSEQHYGYALSASASAVLTRTTAEEALLDALAAPQGDSIVV
                     FNPLSWPRTDLVRVVLPSALFESEFTLQDSLGNVPYQKIPETGQIVFVADQVPSVGFK
                     RYWIAAGSPPATSSSLVVGAQSIENSRYRITVNANGHVTSFQDKVAARELINTGSSLN
                     FNRVVVATNIEHFFGTHRVVPDSVSTTISAEMTGPVAASLRIENASHPLKSVRIVLYD
                     QLDRVDLVNTVDRQQMEYAPLSVNSIYYALTYPLQLTGHKVRIDTAAGWLDPAVQSLP
                     GSYRGSHCIQHALDISESGYGV"
BASE COUNT          286 a          344 c          339 g          275 t
ORIGIN      
        1 caacgccgta gcccgattca gaaatgtcca gtgcatgctg gatgcagtgt gagcctcggt
       61 aggagccggg cagactctgt accgctggat cgagccaacc tgctgcggtg tctattctaa
      121 ccttgtgacc tgtcagctgc agtggatacg tcagcgcata gtaaatcgaa ttgacactca
      181 aaggcgcgta ctccatctgt tgacgatcca ccgtgtttac cagatctact cgatccagtt
      241 gatcgtagag gacaatcctc acacttttga gagggtgaga ggcgttttcg atcctgagac
      301 tggcagcaac gggacctgtc atctcagcgc tgatcgtcgt tgagacagaa tcgggcacca
      361 cacggtgcgt gccgaaaaaa tgctctatat tggtcgccac aacaacacgg ttgaagttca
      421 gagaagagcc agtgttgata agctctcgtg ctgccacttt gtcttgaaaa ctggtcacgt
      481 gaccattggc gttgacagtg atgcggtaac ggctgttctc gatgctctgt gctccaacga
      541 ccagcgaaga agaggtggcc ggcggtgaac ccgcagcaat ccaatagcgc ttgaagccaa
      601 ctgacggaac ctgatcggca acgaaaacaa tctgtcccgt ttcaggaatc ttctgatagg
      661 gcacgttgcc gagagaatcc tggagtgtaa attcgctctc aaagagtgct gaaggcaaca
      721 caacacgcac caggtctgtc cgaggccagg acagcggatt gaaaacgacg atggagtccc
      781 cctggggagc cgccagggca tccagcagag cctcctcagc agtggttcga gtcaagacgg
      841 cagaggccga cgcgctcagg gcgtacccat agtgctgctc actgttggcg tccacttctt
      901 cctgcgtgaa gtaatccgcc catcctccac cgccaccgga gtgctcatcc accgtgagca
      961 tcatatccca ggcgttggtg aattcatcat ggggataggt tcccaacccg acttccgaag
     1021 cgatcgccca catcttctcc gttgccaaga cttcatcctg agcgttcttg acgatcttct
     1081 cagacttggg ctccgtcatc gagcccccgt cccagaggga tgtccagttg ccagaatgct
     1141 cgggtatctg actgtcgtag ttggcgacca ggtactcaaa gaactcctcg ggcgtggcaa
     1201 ggatgaactt gggattgtcg ttgttggcat tccaagtctg aatc
//
LOCUS       DAOQUC010001416         1242 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_159560_length_1242_cov_2.286436, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001416 DAOQUC010000000
VERSION     DAOQUC010001416.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1242
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_159560_length_1242_cov_2.286436"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            35..>1242
                     /locus_tag="PKH07_18750"
     CDS             35..>1242
                     /locus_tag="PKH07_18750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37037.1"
                     /translation="MHIQVCHFATADALLVLFVTAALHSFIQVADRGSLWNYLCSAVI
                     TALVLATDPVLLPILLVFVGAFLLSLITENRFRTIKPYLGLALFLATFFVTLFVTHPF
                     AFLQNLPVSIGERAPGFSALWATVAPRLRILGSTDFWAGIRERVDWLSGLPSNATTLP
                     YLRTFPYLYPAANLCRWCLGWPLGLAVIAGLFYFTGKHLYGWDRSSLVLLGWTLPYLA
                     ITGRMTVKQPGTLMPVLPCLCLFAAVLLSDLIRLLGFSDERRPASVQMADLLGIRTLS
                     RRFCLWLGTFGLLGMLLVVCFSAYYSLQFVEIYRQPHTWMKASAWIYQNVPTEKKIVC
                     EQLDQPLPRDIAEHSADKFAYLINPVYDEPDWDTNKISLLAENLLKGDYLVLGSKRSY
                     AAMLRLPSRFP"
BASE COUNT          211 a          361 c          326 g          344 t
ORIGIN      
        1 ccttcttgca tccgtccttc ttggcgtttc agtcatgcac attcaagtgt gccacttcgc
       61 tacggccgat gcattgttgg tgctcttcgt aactgcggcg ctccacagtt tcatccaggt
      121 cgccgacagg ggatctcttt ggaactatct ctgtagcgct gtcattaccg cgctggtgtt
      181 ggcaaccgat ccggtgctgt tgccaattct gttggtcttt gttggtgcgt ttctgctgtc
      241 cctgatcaca gaaaacaggt tccgaacgat caagccttac ttgggacttg ctttgttcct
      301 ggcaacgttt ttcgttacgc tgtttgtgac tcatcccttc gcatttcttc agaatctgcc
      361 ggtaagcatt ggcgagcggg ctcccggatt ctctgcactc tgggcaacgg tcgcgccacg
      421 tcttcggatt ctgggcagca ccgacttctg ggctggcatc cgtgagagag tggattggct
      481 ctcaggactg ccgtccaacg cgaccacact cccctatctc cgaacgttcc catacctgta
      541 tccggctgcc aacctgtgcc gatggtgttt gggatggccc cttggtctgg ccgtgatcgc
      601 cggtctgttt tatttcacgg gcaagcatct gtacggttgg gatcgttcga gtctggtact
      661 gctggggtgg acgcttccgt atctggcaat caccggtcgg atgaccgtca agcagcccgg
      721 cacgctcatg ccggtgctgc cgtgtctgtg tctttttgcg gcggtgctcc tgtccgacct
      781 gatccgcctg ctgggtttct ccgatgagag aagacccgct tcggttcaga tggccgatct
      841 gctcggtatt cgcacgttat ccaggaggtt ctgtctctgg cttggcacgt tcggacttct
      901 gggaatgctt ctggtcgtct gcttcagcgc ttattactcg cttcagtttg ttgagattta
      961 cagacaaccc catacctgga tgaaggcatc agcgtggatt tatcagaacg ttcccaccga
     1021 gaagaagatc gtttgcgaac aactcgacca gccacttccc cgagacatcg cagagcactc
     1081 cgccgacaag tttgcctatc tcatcaaccc cgtctatgac gagccggact gggataccaa
     1141 caagatttca cttttggccg agaaccttct caagggcgac tatcttgttc ttggcagcaa
     1201 acgcagctat gccgcgatgc tccgacttcc gagccgattt cc
//
LOCUS       DAOQUC010001417         1241 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_159679_length_1241_cov_3.085160, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001417 DAOQUC010000000
VERSION     DAOQUC010001417.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1241
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_159679_length_1241_cov_3.085160"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..775
                     /locus_tag="PKH07_18755"
     CDS             <1..775
                     /locus_tag="PKH07_18755"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HNT37038.1"
                     /translation="YRDGNPTIEDCLIAGNRGGGVACYQSSPSLTNCVLTGNQAYQGG
                     GLFCQQGATPLVTNCTLAGNISSGGFTHAPGGAGVDSESSSPALFNCILSGNQGYGIR
                     QSNSHPPMTISNCLFDANSSGDYYDQDIGAVTGANTINMEVDGASNNREGDPSFLMGA
                     SGTWSASSVFDATTQKTTLTNASASYLPGALVGKTLQCLASFPYESVVVANTATQVTV
                     SGNLTNYNISDGSPYLFVDHHLTNGSSALDRAQLSTILV"
     gene            complement(734..>1241)
                     /locus_tag="PKH07_18760"
     CDS             complement(734..>1241)
                     /locus_tag="PKH07_18760"
                     /inference="COORDINATES: protein motif:HMM:NF033564.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HNT37039.1"
                     /translation="EQAFEDYAGECRKEQYAQDTSVDHGRIEERICRTLNAERLPEWV
                     PREDWVGLNTIIQIQSERTIMANGTVTQETRYYISSLRLSADRIASYVRGHWGIENQL
                     HYVLDVFWGEDGSRKRTACAAANFGTVLRIAHNLVKTHPENISIKRKRKKADRSDQYR
                     GQLGSIEC"
BASE COUNT          269 a          349 c          294 g          329 t
ORIGIN      
        1 ctatcgggac ggcaacccta cgattgaaga ctgtctcatc gctggcaaca gaggtggtgg
       61 tgttgcctgt taccagtcct ctcccagttt gacgaactgc gttctgacgg ggaaccaggc
      121 gtaccaaggt ggcgggctct tttgtcagca aggtgcaacg ccgttggtca ccaattgtac
      181 tcttgcaggc aatatctcct ccggcggttt cacacacgct ccaggaggcg ctggtgttga
      241 ctctgaatcg tcctctccag cgctgttcaa ctgcatcctt tcaggcaatc agggctatgg
      301 cattcgacag tcaaactcac acccgcccat gacgatatcc aactgtcttt ttgatgccaa
      361 ctccagtggc gactactacg accaggatat tggcgctgtt acgggtgcca acaccatcaa
      421 catggaagtt gacggggcga gcaacaaccg agaaggagat ccttcgttct tgatgggggc
      481 atctggaaca tggagtgcct cctccgtctt cgatgccaca actcagaaga cgacactcac
      541 gaatgcgagc gcctcctatc ttcctggggc tttggtcggt aagacacttc agtgtctggc
      601 gtccttccct tacgagtctg ttgtagtggc aaacacagcg acgcaagtca ctgtcagtgg
      661 aaatctcacc aactacaaca tctctgacgg aagtccctac ctcttcgttg accatcatct
      721 gacgaatggc tcttcagcac tcgatcgagc ccagttgtcc acgatattgg tctgatcgat
      781 ctgccttttt tcgtttccgt ttgatactga tgttttcggg gtgtgttttg accagattat
      841 gagcaattct gagtactgtg ccaaaattag cagcggcgca agcagttcgt tttcggctcc
      901 cgtcttcgcc ccaaaacaca tctaacacat aatgcaattg gttttcaatc ccccaatgcc
      961 ctctgacata cgatgctatg cggtcggcag acaggcgaag gctgctgatg taatatcttg
     1021 tttcttgagt gacagtgcca ttggccataa tcgttcgttc gctctggatt tggatgatcg
     1081 tatttagtcc gacccagtcc tccctcggaa cccactccgg cagcctttcg gcgtttaaag
     1141 tccggcaaat gcgctcctca atacgaccat gatctaccga agtatcctgt gcatattgct
     1201 ctttccggca ttcgcccgca taatcttcaa aagcctgttc t
//
LOCUS       DAOQUC010001418         1240 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_159882_length_1240_cov_3.349367, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001418 DAOQUC010000000
VERSION     DAOQUC010001418.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1240
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_159882_length_1240_cov_3.349367"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..910)
                     /locus_tag="PKH07_18765"
     CDS             complement(<1..910)
                     /locus_tag="PKH07_18765"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HNT37040.1"
                     /translation="MINRRQALQAISLGIAGSLGCAQGVLNPVGLGKGSQRSAKEKPN
                     ILFAIADDWSWPHAGVYADRVVKTPVFDRIAHDGILFTNAYCAAPTCTASRGAVLSGQ
                     AIHRLGEGANLHSTLTPDIPLYTDLLEANGYAVGMTGKGWAPGNFQAGGRTRNPAGTA
                     YKDFGSFLDQKQPDQPFCFWFGSINPHRPYDRDSGVRSGMSPDAVEVPPFLPDTPEVR
                     RDILDYYYEVQAFDTEVGRLLDTLEARGLSDNTLIVMTSDNGMPFPRAKTTLYGSGTH
                     MPLAIHWPAGIQNPGRVVDGLISFTDF"
BASE COUNT          282 a          341 c          315 g          302 t
ORIGIN      
        1 cgaaatcagt gaaactgatc agaccatcaa ctacccgtcc gggattctga atgcctgccg
       61 gccagtgtat tgccagaggc atgtgggttc cagaaccata gagcgttgtc ttggcacgtg
      121 ggaacggcat tccattgtcg ctggtcatca cgatcagagt gttgtcagac aggccacgcg
      181 cttccagagt gtccaggagt cgccccacct cggtgtcgaa cgcctgaact tcatagtagt
      241 agtcaaggat gtctcttcgc acctctgggg tatccggtag aaacggtggc acctcgaccg
      301 catcgggtga cattcccgaa cgaacccctg aatccctgtc atagggtcgg tgaggattta
      361 tgctgccaaa ccagaaacag aaaggctgat caggctgctt ttgatcaaga aaagagccga
      421 agtctttgta agccgttcct gcaggattcc gtgttcgccc gcccgcctgg aaattccctg
      481 gtgcccaacc ctttcctgtc atgccaacgg cgtagccgtt cgcctcaagc aaatctgtgt
      541 acagcgggat atctggagtg agtgtactgt gaaggttggc accctcccca agcctgtgaa
      601 ttgcctgtcc agacagaact gcacctctgg atgcagtaca ggttggtgca gcgcaatacg
      661 catttgtgaa aaggatccca tcatgagcga tacggtcgaa aacgggcgtc tttacaacgc
      721 gatctgcata gactccagca tgtggccaag accagtcatc tgctatggca aacaggatgt
      781 ttggtttttc ctttgcagat ctttgagaac ccttccccaa accaaccgga ttcagaacgc
      841 cctgagcaca accgagactt ccggcgatcc ccaaggatat cgcctgaagt gcctgtcttc
      901 tgttgatcat gactgtccac ctttattgcc cttttatcga atggggtagt ctgaaatctg
      961 atcaagccat tgtcaagaga gtttggactg acttaagcat cctgcctgct gcaattgaca
     1021 caatccagac gcgtaaccgg aatcgtgata ttgttccgac tgaccgttga cacgtcacgc
     1081 aagtcaacgt cacaggaggt ctcactccat ggcacctttc agaggcattt acctcactgg
     1141 ctttctggct gtacttggca tggcggttgc gctgccggtt cacgccagcc cgatctggac
     1201 tcatgaatct tctggtgatg cggtactctg tcttacgtcg
//
LOCUS       DAOQUC010001419         1238 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_160392_length_1238_cov_2.643280, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001419 DAOQUC010000000
VERSION     DAOQUC010001419.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1238)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1238)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1238
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_160392_length_1238_cov_2.643280"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..355
                     /locus_tag="PKH07_18770"
     CDS             <1..355
                     /locus_tag="PKH07_18770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37041.1"
                     /translation="LTSRFRAGEFEMGMLDINIMTPDPDSALYWFQHTNTSLNLGYDN
                     PRVDELLDNARSDQDQEARKAMYEEVLRNVLDDAPYIYTCHRNMAWAYNRNLTGFLTP
                     KWQGMINLAPVHWTA"
     gene            724..>1238
                     /locus_tag="PKH07_18775"
     CDS             724..>1238
                     /locus_tag="PKH07_18775"
                     /inference="COORDINATES: protein motif:HMM:NF040248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNT37042.1"
                     /translation="MISNILSRVVEILSSLLVISVLVFLLIQIVPGDPIVQMLGENTT
                     PTIVEEMRSYYGLDRPLHVQYLSWLQRVLHGDLGRSIRTRLEVSKEIMERFPSTFLLA
                     ISATALSTVLAVPIGILSAYRMNSLVDYAVRATSMVGVAMPGFVVAVLLILILSVKLD
                     LLPISGAPAML"
BASE COUNT          280 a          312 c          343 g          303 t
ORIGIN      
        1 cttgaccagc cgcttcagag ccggggagtt cgagatgggg atgcttgaca tcaacatcat
       61 gacaccggat ccggattctg cactctattg gttccagcac actaacacta gcctcaatct
      121 tggctatgac aatcccagag tagatgaact cctggataat gcacgatcgg accaagacca
      181 agaagccagg aaagccatgt atgaggaggt tctccgaaac gtcctcgacg acgctccgta
      241 catctacact tgtcaccgaa acatggcatg ggcctacaat cggaatctga ccggcttcct
      301 taccccgaaa tggcagggga tgataaacct cgccccggtt cattggacag cataagggga
      361 taaactcacg tagccatgtc tgggcatggg cattgtatcg cagcgcccag acatggctac
      421 aggtgcttct gattcggagc gtgcaggctt tctgcaaaca gctttggggt tttcggtgag
      481 catctgatcg gctgcgccag cgtctcgatg ctaaccacgc ctgtctttga ttctgcggga
      541 aggtcagcga cggcaatctg ggtgtcactg atgttggatt acgcgcactg tatccaagag
      601 gatgaaacgc gtggcggtgc tggtggcatg cccgcattca gatgtacagt aacgtatcac
      661 ggttgctctt gtccctccaa tgagagcgat gacacacctt gaagacggag agatagccgc
      721 caaatgataa gcaatatact gagcagagtg gttgagattc tgtccagcct gctggtgata
      781 tcagtccttg tcttcctatt gatacagatc gttcctgggg atccgattgt gcaaatgctg
      841 ggtgagaata cgacaccgac gatagtcgaa gagatgagga gctactacgg actagaccga
      901 cctctacacg tgcagtactt gtcgtggcta cagcgcgtct tgcatgggga tcttggtcgt
      961 tccatccgca cacggctcga ggtcagcaaa gagatcatgg aacgatttcc ttcgacgttc
     1021 ctgctggcga tcagtgcgac agcgctctct acggtacttg cagtgccgat tgggattctc
     1081 tcggcatata gaatgaactc gcttgtggac tatgctgtcc gtgccacgtc catggtcggt
     1141 gttgcgatgc cagggtttgt ggtggcggtt ctgttgattc tgattttgtc cgtcaaactg
     1201 gaccttttgc cgatttctgg ggcacctgca atgctgaa
//
LOCUS       DAOQUC010001420         1237 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_160574_length_1237_cov_3.148900, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001420 DAOQUC010000000
VERSION     DAOQUC010001420.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1237
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_160574_length_1237_cov_3.148900"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..493)
                     /locus_tag="PKH07_18780"
     CDS             complement(<1..493)
                     /locus_tag="PKH07_18780"
                     /inference="COORDINATES: protein motif:HMM:NF019342.2"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HNT37043.1"
                     /translation="MERPVIGISTKTRFVGGTTGTSANTEIQYSPLEYMRSVWNAGGN
                     PMLLPLISDESAIDAWLERLDGVILSGGMDVDPIHYGQEPSKLFGGIDGPKDGMELIL
                     GKRLLETGLPTFAICRGIQLVNVVAGGTLFQDVSMAAETPLKHSQSTTDERPTHSITI
                     EKGS"
     gene            complement(569..>1237)
                     /locus_tag="PKH07_18785"
     CDS             complement(569..>1237)
                     /locus_tag="PKH07_18785"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-rhamnosidase C-terminal
                     domain-containing protein"
                     /protein_id="HNT37044.1"
                     /translation="LLPKSVQPLTVRLLLKDIEERDGHLSTGFVGLPILLKTLTKFGH
                     ADVAYKLLLNDTFPSWGYEIKHGATTIWERWDGWTQEKGFQDPGMNSFNHYAFGAVGE
                     WLFATVAGIDLEEPGFKKIHIRPCPDGRISSVKATYRSINGEIVSEWRQTQDKFALSV
                     TIPPNTEAAVSIPAASPSHVRENGIVLEESEGVHSVEAGDGVVIVRVGSGRYRFVSKR
                     KRSQ"
BASE COUNT          307 a          364 c          275 g          291 t
ORIGIN      
        1 gacttccctt ctcaatggta atgctgtgag tcggacgctc atccgtcgtg ctttgtgagt
       61 gctttagagg agtctcagcc gccatactca cgtcctgaaa aagtgtaccg cctgccacaa
      121 cattcaccag ctgaatcccc ctgcagatgg caaacgttgg aagtccggtc tccagaagtc
      181 gtttccccag aatcagttcc atcccatcct tcggaccatc aataccccca aagagtttcg
      241 acggttcctg accatagtga atcggatcca catccatacc gccagaaagt atcactccgt
      301 ccaatcgctc aagccatgca tctatggcgc tctcgtccga gatcaaagga agcagcatcg
      361 ggtttccacc tgcattccac actgacctca tgtactccaa aggagagtat tgaatctccg
      421 tgtttgcgga agtccctgtg gttccaccaa caaaacgcgt cttcgttgaa atgccgataa
      481 caggtcgctc catcaaatcc tccgttgact ctctctatgc tagagcccgg aactaaatat
      541 ccgttctcct ttgttccggc ttcataatct actgggaccg cttcctctta gagacaaacc
      601 ggtaacgtcc cgatccaacc ctgacgatca cgacaccgtc ccctgcctcc acagaatgaa
      661 ccccctcaga ttcctccaac acaataccgt tctctcgcac gtgagaagga gaagccgccg
      721 gaatgctcac cgcagcctca gtgttggggg gaatggtgac agagagggca aacttgtctt
      781 gggtttgtct ccattcagag acgatctcac catttatcga gcggtacgtg gccttgactg
      841 aagagattct cccgtcagga caaggacgga tatgaatctt cttgaaacct ggctcttcaa
      901 ggtctatgcc cgcaaccgtg gcaaagagcc attctccaac agcaccaaag gcgtagtggt
      961 tgaaggagtt cataccagga tcctgaaaac ctttctcctg ggtccagcca tcccatcgtt
     1021 cccagatcgt tgtggcacca tgctttatct catagcccca cgacgggaaa gtatcattga
     1081 gcagtagttt gtacgcaaca tctgcgtgac caaacttcgt cagggtcttc aggagaattg
     1141 gaagtccgac aaaaccggtc gaaagatgcc catcgcgttc ctcgatatcc ttgagcaaga
     1201 gtctgacggt aagaggctga acactctttg gcaacaa
//
LOCUS       DAOQUC010001421         1236 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_160793_length_1236_cov_3.580017, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001421 DAOQUC010000000
VERSION     DAOQUC010001421.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1236)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1236)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1236
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_160793_length_1236_cov_3.580017"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..597)
                     /locus_tag="PKH07_18790"
     CDS             complement(<1..597)
                     /locus_tag="PKH07_18790"
                     /inference="COORDINATES: protein motif:HMM:NF013067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37045.1"
                     /translation="MKILITNVGSTSLKFKLFLMEEGKEEILLQGGMERIGQQGEGVY
                     KWRSASGKKFEGTKVLANYSEAIALLLEILAENGMKDLTTLAGIGFKVVHAKDVTGCV
                     VLDENVLKAMEAFNTIAGAHNPPYILAIRTFAQIVPQVPLVGLFETAFHVNIPEHAYT
                     YGLPYDLAKKHGIRRYGFHGASHRYVSERMLAQIPQRPL"
     gene            complement(695..>1236)
                     /locus_tag="PKH07_18795"
     CDS             complement(695..>1236)
                     /locus_tag="PKH07_18795"
                     /inference="COORDINATES: protein motif:HMM:NF039902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37046.1"
                     /translation="RSNTPADLWHFWKPFVLDAGLAEVVERTEGVLEGVYLCPSQWGE
                     KAEFRPAEWVFEVMQTLQPRAKTLVEMTQRARFYFVKDIQFDEVAVAKNLKPEAKVPM
                     QDLIQGLEGLSEPWQQADLENLFGQIIERHGIKMVKLAMPVRVGLTGESVSPGIFEIV
                     KLLGRALSLERLQKAMERI"
BASE COUNT          282 a          351 c          289 g          314 t
ORIGIN      
        1 taagggacgc tgaggaatct gagcaagcat cctctcgctg acgtatcggt gcgatgcacc
       61 gtgaaaacca tagcggcgta tgccatgctt cttcgccaag tcgtagggca agccataggt
      121 gtatgcgtgc tccggaatgt tgacatggaa tgctgtctcg aaaagtccga ccagtggcac
      181 ttgaggaacg atctgagcaa acgtccgaat ggcgaggatg tacggaggat tatgcgctcc
      241 cgcgatcgtg ttaaaagctt ccatggcttt cagcacattc tcatccagaa caacgcatcc
      301 tgtgacatct tttgcatgaa ccaccttaaa gccgattcct gccagcgtcg tcaagtcttt
      361 cattccgttc tcggccagaa tctccagaag cagcgcgatg gcttcgctat agttggcaag
      421 cacctttgtg ccttcgaact tcttccccga tgcggagcgc catttgtaga cgccctcacc
      481 ttgctgtccg atgcgttcca tacctccctg gagaagaatc tcctctttgc cttcttccat
      541 gagaaagagt ttgaacttca aagacgtgct tccaacattg gtgatcagga ttttcacagt
      601 ttcatcttcc tttccgttta gtaactcgct atgaaccttc agggttacag gctgatattc
      661 agcaacaaga gcgacgaaaa gtccagcaga aaaatcagat tcgttccatc gctttctgaa
      721 gtctttcgag tgatagggct ctccccagca atttgacgat ttcaaagatt cccgggctga
      781 ccgattcccc cgtcagtcct actcgcacag gcattgccaa cttgaccatc ttgatcccgt
      841 gtctttcgat gatctgtccg aacaggttct ccaaatccgc ctgctgccat ggttcgctca
      901 aaccttcaag accctgaata aggtcttgca ttggaacctt ggcttcgggc ttgaggttct
      961 ttgcgaccgc aacttcgtcg aattggatat ctttgacgaa atagaaacgt gcccgctgag
     1021 tcatctcgac aagcgtcttc gctcgcggtt gcagtgtttg catcacctca aagacccact
     1081 ccgcagggcg gaattccgcc ttctctcccc actgtgaggg acagagatag acgccttcga
     1141 gcaccccctc tgtgcgctcc acgacctctg ccagccctgc atcaaggaca aacggtttcc
     1201 aaaaatgcca caggtctgcg ggagtattgg accgga
//
LOCUS       DAOQUC010001422         1236 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_160832_length_1236_cov_2.633362, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001422 DAOQUC010000000
VERSION     DAOQUC010001422.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1236)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1236)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1236
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_160832_length_1236_cov_2.633362"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..421)
                     /locus_tag="PKH07_18800"
     CDS             complement(<1..421)
                     /locus_tag="PKH07_18800"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF014726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HNT37047.1"
                     /translation="MNWRVEVTAKKVALDSEGKDVLSNARDLSLKGLDSIRTGWIYVL
                     DDTLDASKVALLADRLLSDPIEHQYVILEGDDCDSALVPGSRWEVEVHYKKGVTDTTA
                     ESLARGAEDLGITNLKGIRTGRIYWLFGTISLDDANRL"
     gene            complement(593..1147)
                     /gene="purE"
                     /locus_tag="PKH07_18805"
     CDS             complement(593..1147)
                     /gene="purE"
                     /locus_tag="PKH07_18805"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010868758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HNT37048.1"
                     /translation="MSKQTTKQAINPQVGIIMGSDSDLETVVHAAQTLDNFGVPSDLV
                     VASAHRSPALVTKTLRSWEKAGVQVVIAAAGCAAHLPGVVASQTYLPVIGVPLAAPPF
                     NGMDSVLSILPMPGGIPVATMAVGKPGVINAALFAVRILALHNPILGTKLASHIKDME
                     RSVVEKNKKLKSLGAKAFLAQRGK"
BASE COUNT          275 a          365 c          301 g          295 t
ORIGIN      
        1 ctaaccgatt ggcatcgtcc agggagattg ttccaaaaag ccagtaaatt ctgcctgtgc
       61 gaatcccttt gaggttggtg attcccaagt cctcagctcc gcgcgcaagg ctttccgctg
      121 tcgtatcggt cacgcctttc ttgtagtgga cttccacttc ccagcgtgag ccaggaacaa
      181 gagcgctgtc gcagtcgtca ccttccagta tgacatattg atgctcgatg gggtcggaca
      241 gaagccgatc cgccagcaat gcaaccttgg atgcgtccaa agtgtcatcc agaacataga
      301 tccaaccggt gcgaatggaa tcgagtccct ttagactcaa atctcgggcg ttggaaagaa
      361 cgtccttgcc ttcactgtcc agtgccactt tcttggctgt gacttcaact cgccagttca
      421 ctgttgtccg tcctgatgct tctttgaaaa ccagacctga gaagcatctg atttctacct
      481 gaaacgggcc cctgcatggg caggcgcagc ctgatgatca tgcacgttct tgtccgtatg
      541 ggaaaacacg cttctccgcg ttcgtgtctc ccaatagagg aatgcgcatt ccctactttc
      601 ctcgttgtgc gagaaacgcc tttgccccaa ggctcttcag tttcttgttc ttctcgacga
      661 ctgaacgctc catatcctta atatgtgagg ccaacttcgt tcctaagatc ggattgtgta
      721 gcgccaggat gcgaacggca aagagcgccg cgttgatcac tcctggcttt ccgaccgcca
      781 ttgtcgccac gggaatccca ccgggcatgg gcaatatcga aagcaccgaa tccatcccgt
      841 tgaaaggtgg tgctgccaaa ggtactccga tgacgggcag ataagtctga ctggccacaa
      901 ctccgggaag atgcgcggca caacctgccg cagcaatcac aacctgaact cccgctttct
      961 cccaagaccg aagggtcttg gtgaccagag ccggtgagcg gtgcgcggac gccacgacta
     1021 aatccgatgg caccccaaaa ttgtccagag tctgagcggc atgcacaaca gtctccagat
     1081 cgctgtcgct tcccatgatg atcccaacct gaggattgat cgcctgcttt gtagtctgct
     1141 ttgacactga ttccaactcc cctgctatcg cgatgattca aggaatatcc gacgatagac
     1201 ttcctcgtaa gactcctcga ctttgcccag atcccg
//
LOCUS       DAOQUC010001423         1234 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_161291_length_1234_cov_2.542833, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001423 DAOQUC010000000
VERSION     DAOQUC010001423.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1234)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1234)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1234
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_161291_length_1234_cov_2.542833"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..913
                     /locus_tag="PKH07_18810"
     CDS             <1..913
                     /locus_tag="PKH07_18810"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNT37049.1"
                     /translation="GLARLPDVLRDCLRIALVRGGECTDQPLDISGTDVRGSMLSLRM
                     STRLIRDSQGTLQGVVAIFEDLSALRHAEEQLWRSERLISVGQLAAGLAHELGNPIGI
                     IKSCAEYLRRKLPPDSQLQEDLQVIDTESDRCQALIKQLLSLASQDEIHLESADLSIV
                     VERALPLVRYGRQAESLHFELVLHKTPIPVCIDENLFIQSLVNVLLNAVQASKEGGTI
                     RIRTSLDEGEVPSALVSISDEGCGIDEETQKRIFDPFFTTKETGSGLGLSITHRIIER
                     LGGRIQVRSQLHQGTTLEILIPVHQQA"
     gene            complement(986..>1234)
                     /locus_tag="PKH07_18815"
     CDS             complement(986..>1234)
                     /locus_tag="PKH07_18815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37050.1"
                     /translation="RIGKERWNMLIPEYHFHLFTAKSLGDLMKDTGFTAILTRTVSGS
                     GEEQGLRRWTALSKELGLSVFRLGNALLVLSRTVERAG"
BASE COUNT          283 a          324 c          320 g          307 t
ORIGIN      
        1 aggcctggcg cggcttcctg acgtgttgcg tgactgtctg cggattgctc ttgttcgagg
       61 cggcgaatgc acggatcaac ctttggacat ttccggaacc gatgtccgag gaagcatgct
      121 ctccctgcgg atgagtacca ggctcatccg ggattctcaa ggcactctgc agggcgtcgt
      181 ggcgattttc gaagacctct ctgcacttcg ccatgcggaa gagcaactct ggcgctcaga
      241 gagactcatc tcggtcggtc agttggcagc aggccttgcc catgaactgg gtaacccaat
      301 tgggatcatc aaatcctgtg ctgagtacct gagaagaaaa ctgcctccag acagtcagtt
      361 gcaggaagac ctgcaagtga tagacactga atctgaccga tgtcaggctt tgatcaagca
      421 attgctctca ctggcatctc aagatgagat tcatctggaa tcagcggatc tgagcattgt
      481 tgtcgagcgg gcgcttcctt tggttcgcta cggccggcag gcggagagtc ttcacttcga
      541 actggttttg cacaagacgc ctatacctgt ttgcattgat gagaacctct ttatccagtc
      601 tcttgtcaac gtccttctga atgccgttca ggcatcaaaa gagggtggaa cgatccgcat
      661 ccggacatct cttgatgagg gtgaagtccc cagtgccttg gtttccatct ccgatgaagg
      721 atgtgggata gatgaggaaa ctcagaagcg catttttgat ccatttttta cgaccaagga
      781 gaccggatcg ggactcggtc tttcgattac acatcggatc attgaacgcc tgggcggtcg
      841 catccaagtc cgcagtcaac tccatcaggg aaccactctg gagatcctta tccctgtcca
      901 tcagcaggca tgactcgaac tgaccctcaa gggaaatcgg gtctgaatca gctacacctg
      961 ctcaagagta ttctttgatg gcgatttatc cggcacgttc gactgtgcga gacaggacta
     1021 acagtgcatt ccccagacgg aagactgaca gccccagttc cttggaaaga gctgtccagc
     1081 ggcggagacc ctgttcttca ccacttccac tcaccgtcct ggtcagaata gctgtaaatc
     1141 ctgtgtcttt cataaggtct ccaagactct tggctgtgaa gagatgaaaa tgatattcgg
     1201 gaatcagcat attccatcgt tctttcccga ttcg
//
LOCUS       DAOQUC010001424         1232 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_161753_length_1232_cov_2.451997, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001424 DAOQUC010000000
VERSION     DAOQUC010001424.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1232
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_161753_length_1232_cov_2.451997"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="PKH07_18820"
     CDS             <1..372
                     /locus_tag="PKH07_18820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013953143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HNT37051.1"
                     /translation="YIAQANREIFVVAQIEDVEALDTLDDIAATPGIDILFLGAQDLS
                     QSMGIPCQFDHPRIVQARKRVAEAARKYNKWWGCPAGVEKAEILYKEEGAQFFAGCAA
                     IWFVLDGLKQVRAEFTKRLGD"
     gene            503..>1232
                     /locus_tag="PKH07_18825"
     CDS             503..>1232
                     /locus_tag="PKH07_18825"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37052.1"
                     /translation="MQATAVVKGLGTLDFSVMAIYLVLVFALGALFARQQHSTRDFFL
                     AGRSMGILPIALSLIATIFSAISFIAGPAAVCIYGLAMLGGTFVIFLMIPIVTKVFIP
                     FYSRLKVYSAYEYLERRFDVRVRCLASGMFIVWRLFWMGLALYSPCLALHSAMGSESQ
                     QILYVMIITLGLVATAYTVMGGMKAVIWTDVVQFCIFFGAIGLAIIYTATHVDGGVAG
                     IWAFAREKGRTRLTVDPSAHAEFRS"
BASE COUNT          231 a          302 c          359 g          340 t
ORIGIN      
        1 tacattgccc aggcaaacag agagatattc gttgtcgctc agattgaaga cgtcgaggct
       61 ctggatacct tggatgacat cgccgcaacg ccgggcattg acattctttt ccttggcgca
      121 caggacttgt cgcagtccat gggaattccc tgccagtttg atcatccgcg catcgttcaa
      181 gcgcgcaagc gggttgccga ggcggcgcgc aagtacaaca agtggtgggg ttgtccagca
      241 ggcgtcgaaa aggcagagat cctctacaaa gaggaaggcg ctcaattctt tgctggttgt
      301 gcggcgatct ggttcgtcct ggacggtctg aagcaggttc gcgcggaatt caccaaacgc
      361 ctgggagact gacgttatgc gtcggcttcg atagcagaca tttgctgagg tcactccctg
      421 acagggggtg acctcggttg tttcgaagtt gtgatctgaa ctgtcttgag agttctgagt
      481 tcgggcagaa gggggtatgg acatgcaggc aaccgcagta gtgaaagggc ttggaacgct
      541 ggatttcagt gtgatggcca tctacctggt gttggttttc gccttgggcg ctctgtttgc
      601 aaggcaacag cattccacca gggatttctt ccttgccgga cgttcgatgg gcatccttcc
      661 catcgcgctc tcactcattg ccacaatctt ctcggcgatc tcattcattg ctgggcctgc
      721 cgcagtctgc atctacggac ttgccatgtt gggtggaacc tttgttatct ttctcatgat
      781 tccgatcgtc accaaggtct ttatcccgtt ctactcacgg ctgaaagttt acagtgccta
      841 tgaatatcta gagcgacgtt tcgatgtccg ggttcggtgt ctggcgtctg gcatgttcat
      901 tgtttggcgt cttttctgga tgggactggc attgtactct ccctgtctcg ccttgcactc
      961 tgcaatgggt tcggagagcc aacagattct ctatgtgatg atcattactc tggggcttgt
     1021 ggcaacagct tatacggtca tgggtggtat gaaagcggtt atctggacag atgtggttca
     1081 gttttgcatt ttcttcggcg cgattggtct ggcgatcatc tacaccgcca cgcacgttga
     1141 tggcggggtt gccggaattt gggcatttgc cagagagaag ggcaggactc gcctgacggt
     1201 cgatccctct gctcatgcgg agtttcgttc gg
//
LOCUS       DAOQUC010001425         1230 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_162141_length_1230_cov_3.517447, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001425 DAOQUC010000000
VERSION     DAOQUC010001425.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1230
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_162141_length_1230_cov_3.517447"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            236..>1230
                     /locus_tag="PKH07_18830"
     CDS             236..>1230
                     /locus_tag="PKH07_18830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37053.1"
                     /translation="MKTLKVKPQFYELQDPREPNLYRDQFPYDRVPMTVFDGVMEIPE
                     LPEDFWITDTTFRDGQQARLPFQVEQVVAIYNMLHRLGGPNGVIRQSEFFLYSPKDRE
                     AVEKCLEKNYPFPQVTGWIRATKSDFKLVKDMQLKSTGILTSASDYHIFLKLKKSRRE
                     ALESYLSIVKEALSNGLSIRCHLEDITRADIYGFCVPFAQELMKLQQESGLKVIIRLC
                     DTMGYGVPLPGVALPRSVPKLVQAMVRDAGVPSECLEWHGHNDFHKVHVNSICAWLYG
                     CSGVNSALIGLGERTGNSPLEAMVIEYMSLTGSTNGMEPKVITELAEYLSQIDCP"
BASE COUNT          307 a          317 c          324 g          282 t
ORIGIN      
        1 tcttattccg atagtcctaa gatagcacaa gacggttttt cgcagaaatg attctgcggg
       61 tggagggcac tggctcagta cgactcacga aacaccaggt tcacggcaga acaaacttga
      121 tatttgtgcc actttggtct atgtatccaa gacctgtatt caggcaggct tacgagcttg
      181 aataaccatg cctcgcgtcg aaacggcctc acgcgggcag gcaaggagag aaagaatgaa
      241 aactttgaag gtcaaaccgc aattctacga gctccaggat ccacgagagc cgaatctcta
      301 tcgagatcaa ttcccctacg atagagtgcc aatgaccgtc tttgatggtg tcatggaaat
      361 acccgagctt ccagaagatt tctggattac cgatacaaca ttccgagatg gacaacaggc
      421 gcgccttcct ttccaggttg agcaagtcgt ggcgatctat aacatgttgc atcgcctggg
      481 tggacccaac ggcgtcattc gtcagagcga gttcttcctc tatagtccaa aagaccgcga
      541 agctgttgag aaatgccttg agaagaacta tccattcccg caagtcaccg gctggatccg
      601 agccaccaag tccgacttca aattggtgaa ggacatgcag ttgaagagca cggggattct
      661 gacttctgct tccgattatc acatcttcct caaactcaag aaatcgcgtc gcgaagcctt
      721 ggaatcgtac ctcagcatcg tcaaagaagc gctcagtaac ggcctgagca ttcgatgtca
      781 tcttgaggat atcacgcgcg ccgacatcta cggtttttgt gttccgtttg cgcaggaact
      841 gatgaaactc cagcaggaaa gcggcctgaa ggtaatcatt cgcctgtgcg acacgatggg
      901 ctacggtgtt ccgctgcctg gtgtcgcgct tcctcgaagc gtgccgaaac tggttcaggc
      961 aatggtgcgg gatgctggcg ttccctcgga atgtctggag tggcatggac acaatgactt
     1021 ccacaaagtc catgtcaact cgatctgcgc gtggctctat ggctgttcgg gagtcaactc
     1081 agcgctcatc ggtctgggtg agcgaacagg aaattcgcct cttgaggcga tggtcatcga
     1141 gtacatgagc ctgaccggtt ccaccaatgg gatggagccg aaggtgatca cggaacttgc
     1201 cgaatacctg agccagatcg actgcccgat
//
LOCUS       DAOQUC010001426         1229 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_162359_length_1229_cov_3.450596, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001426 DAOQUC010000000
VERSION     DAOQUC010001426.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1229
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_162359_length_1229_cov_3.450596"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..487)
                     /locus_tag="PKH07_18835"
     CDS             complement(<1..487)
                     /locus_tag="PKH07_18835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020585991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNT37054.1"
                     /translation="MIRNIVTLNILAVVCASQAYAQESFESDVIATASGEIKITFLGH
                     GTLMFDWGGKTIHVDPYSQVADYAKLPKADMVLITHEHRDHLDLAALEKIRTEKTVIV
                     GTKNAAEKIQGIRIMNNGDKVTLDGIPVEAVPAYNIVHKRDNGQPFHPKGDGNGYILT
                     FG"
     gene            complement(590..>1229)
                     /locus_tag="PKH07_18840"
     CDS             complement(590..>1229)
                     /locus_tag="PKH07_18840"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HNT37055.1"
                     /translation="GKSIRQEFSASFLCAVPCPQPPTVDGDLSEWQVAAAFHLDREEF
                     SNGTFVGRWTPDDLSASVYLMWDKNNLYVGARVKDQTFNQNYSGESVWMQDSIQLALA
                     PGLDGPTYEFGLALTPTGEQIVQWLPDIKQVTKGTLKVLLESGQQTYEAAIPWSAMPK
                     IGPVKEGIRLRYSLLVNDDDVLVGRRYLESPGGGIAHDKSLENFMEIVLLGQ"
BASE COUNT          294 a          345 c          296 g          294 t
ORIGIN      
        1 ctccaaaggt gagaatatac ccgttgccgt cgcctttggg gtgaaagggc tgtccgttgt
       61 cacgcttgtg aacgatgttg taagccggta cggcttcgac agggatgcca tccagagtca
      121 ctttatctcc gttgttcatg atacggatgc cctgaatctt ctcagcagca ttcttcgtcc
      181 caacgatgac tgtcttttca gtccgaatct tctccaacgc ggcgagatcg agatggtctc
      241 gatgttcgtg agtgattaag accatatcgg ctttgggcag tttggcgtaa tccgccactt
      301 ggctgtaggg gtcaacgtgg attgtcttac caccccagtc gaacataagt gtaccatgcc
      361 cgagaaaggt aatcttgatc tcaccggatg ccgtcgcaat cacatccgac tcaaaagatt
      421 cctgcgcata agcctgactc gcgcacacaa ccgccaagat gttcaaagtg actatgtttc
      481 ttatcatgat gcactcctcc tgcaattatg gtaggaactg cccgaaaaag agtcaagcgt
      541 gtcggcagag gctcgaacgc ctctctcaat gcgtgacaag gtcacacaat cactgcccaa
      601 gcagaacaat ttccatgaag ttctcaaggc tcttgtcatg tgcgatgcca ccaccagggc
      661 tttcaagata acgtctcccg accagcacat catcatcgtt gaccaacaat gaatacctca
      721 gacgtatgcc ctctttcacc ggcccaatct tgggcatcgc agaccaggga atcgctgctt
      781 cgtaagtctg ctgaccgctt tcaagcaaga ctttgagagt gcctttggtg acttgcttga
      841 tgtcaggcag ccactgaaca atctgctcac ctgtgggtgt cagagcaagc ccaaactcat
      901 acgtcggccc gtccaagcct ggagccaacg caagttggat gctgtcctgc atccagacgg
      961 actcacccga atagttctgg ttgaatgtct ggtctttcac cctcgctccg acatagaggt
     1021 tgtttttgtc ccacatcagg tagaccgagg cgctcaaatc atccggagtc cagcgcccga
     1081 caaaagtccc gttggagaac tcttcgcgat ccaggtgaaa tgctgcggca acttgccact
     1141 cggaaagatc accgtctacg gtaggtggct gcgggcatgg aacagcacag agaaaactcg
     1201 cgctaaactc ctgtcgaatt gacttccca
//
LOCUS       DAOQUC010001427         1229 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_162361_length_1229_cov_3.373083, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001427 DAOQUC010000000
VERSION     DAOQUC010001427.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1229
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_162361_length_1229_cov_3.373083"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..346)
                     /locus_tag="PKH07_18845"
     CDS             complement(<1..346)
                     /locus_tag="PKH07_18845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020148159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNT37056.1"
                     /translation="MMKAKDIPRRRFLKSAAVGAIGAALVASTGFGNETKRPNFVILM
                     TDDQRWDAMSCAGNKILRTPNMDRLAKEGVRFENAFVTNSLCTPSRATVLTGLYSHTH
                     GVIDNKGHQIEAD"
     gene            complement(421..>1229)
                     /locus_tag="PKH07_18850"
     CDS             complement(421..>1229)
                     /locus_tag="PKH07_18850"
                     /inference="COORDINATES: protein motif:HMM:NF041540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GC-type dockerin domain-anchored protein"
                     /protein_id="HNT37057.1"
                     /translation="KAGWQHGATVGTDDWGIISFAGANTGSYAWFKNNPTTTSDTWLQ
                     VPAVNLEGKSQLSFWHTFEFEEGGYDGGVLEISTDGGTTWQDLESRIIQNGYNGVISD
                     FGGNPLGVRPAWVSGTIGSMMQVLVDLTDFAGTNRLVRFRFASDSSFGGEGWYIDDII
                     VSSYHQCAEGTPTNTPTTTPTDMPITETPTLTPIDTPTDTPVSETPTDTPTWALSLYD
                     LNSDGAVDAEDLLLVIDLFGTINIQADFNNDGIVDERDLFLFSTHWKENP"
BASE COUNT          276 a          345 c          312 g          296 t
ORIGIN      
        1 ggtcggcctc aatctgatgc cccttgttgt caatgactcc atgcgtatgc gaatagagcc
       61 ccgtcaatac cgttgcacga ctcggagtgc agagggagtt ggtcacaaac gcattctcaa
      121 atcgcacacc ttccttcgca aggcgatcca tgtttggagt acgaagaatc ttgtttccag
      181 cacaactcat cgcatcccag cgttgatcgt ctgtcatcag gatcacgaag ttcggacgtt
      241 tggtttcgtt tccgaagcct gtggaggcaa caagggctgc gccaatcgca cccacggccg
      301 cagatttcag aaatcgccga cgcggaatat cctttgcctt catcatactc gctctcatgt
      361 tgcccagtgc gtccctatgc ttgagctcct ctatcaggaa accacgcaac gaagcgcggt
      421 ctacggattt tccttccagt gtgttgagaa caggaacaga tcgcgttcat caacaattcc
      481 gtcgttgttg aaatctgcct ggatattgat cgtcccaaag agatcgatca ccaacagaag
      541 gtcttcggca tccaccgcac cgtcagagtt cagatcgtag agactgagag cccacgttgg
      601 cgtgtcagtc ggtgtttccg agacaggagt gtccgttggc gtatcaatcg gcgtgagtgt
      661 tggcgtctcc gtaatgggca tgtcagttgg tgtagttgtc ggagtattgg tgggagttcc
      721 ctcagcgcac tggtgatagg aactgacaat gatgtcatca atgtaccatc cttctccgcc
      781 gaatgaactg tccgatgcaa aacggaagcg aaccagccgg ttggtgcctg caaaatctgt
      841 caggtcaacg agcacttgca tcatgctgcc aatcgtccct gacacccatg cagggcgcac
      901 tccaagggga ttcccaccaa agtcagagat gactccgttg taaccgttct ggatgatacg
      961 gctttccaag tcctgccaag tcgtcccacc gtcagttgag atctcaagta caccgccgtc
     1021 atagccacct tcctcaaact cgaacgtgtg ccagaaggac agttgcgact ttccttccag
     1081 attcaccgcc ggcacttgca gccaggtgtc cgaagtggtg gtgggattgt ttttgaacca
     1141 ggcataggaa cctgtattcg ctcccgcaaa ggagatgatt ccccagtcat ccgtaccgac
     1201 ggtcgctccg tgttgccaac ccgctttcg
//
LOCUS       DAOQUC010001428         1227 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_162927_length_1227_cov_2.086177, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001428 DAOQUC010000000
VERSION     DAOQUC010001428.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1227
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_162927_length_1227_cov_2.086177"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            493..>1227
                     /locus_tag="PKH07_18855"
     CDS             493..>1227
                     /locus_tag="PKH07_18855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37058.1"
                     /translation="MRSTLRAIVLASKMGFKATDEDDAIEALSELTGTDAKVSTSAIL
                     HLKDELNILEWDERFKEFVILADAVPRPLFLAWLRQKSATYSESGKAELFAGMSRKPE
                     WSDLLGDIDCDFAEQKKITTKEWRYQGVCTNRENVDVQIKLAADRWEKAIGVDEPRGT
                     IIYCYLARGANPDADSQSIIDRLRDVAKAAATPALPILVVLLYDKEGALGQALAEIAV
                     LDESVGAEERARFGNLIPAHREKMCQI"
BASE COUNT          317 a          272 c          317 g          321 t
ORIGIN      
        1 gttcacctat ctgtcaatct ggaaacactg attgcgaatc tcatcgaaaa gagacagccg
       61 gatctacttg atgaatggtt tgacacgact cgagcaaaag acgtttccat ttccaccatt
      121 aagaatctta gcagatggtt tccgcagtca aaggatcata ggctttggtg cgattcggag
      181 caatttcacc aagttatccg caagggatgt tggccactct ctccattttc cacatggttt
      241 ctatttcatc tagccgcatc tggaaagcac ttacaggagc gttctgcact ttctctgtta
      301 gccgatacgt ttaggcggtt tgaagacgat ttggtagact cttctgctcc atttgcgctt
      361 gcattttcag acttgtggtc tgagtccctt gcgcaagaac tgatgctatc cgaagaggca
      421 ggtcttcagg gctctgtcgc gcatgcatac gtctcagtag aatcaactca cggtgcaaac
      481 ctgacagagg aattgcgaag tacacttcgt gccattgttc tcgcgtcgaa gatgggattc
      541 aaagcaacgg atgaggacga tgctatcgag gctctctctg aacttacagg aacagatgca
      601 aaagtgtcga cctcagcgat tctacacttg aaggatgagc taaacattct tgaatgggat
      661 gagagattca aggagtttgt tattcttgcc gatgccgttc cacgtccact cttcctggca
      721 tggctccggc aaaaatctgc tacctacagt gaatctggta aggcagaatt gtttgcaggc
      781 atgagcagaa aacctgagtg gtctgatctt ctcggagata ttgactgtga ttttgctgag
      841 caaaagaaga tcaccacgaa ggaatggcgc tatcaagggg tgtgtacaaa tcgtgaaaat
      901 gtagatgtgc agatcaaact tgctgcagac cgatgggaaa aggcaattgg ggttgacgag
      961 ccccgaggaa caattatcta ctgttatctt gcgcgaggag cgaatccgga cgctgattct
     1021 caaagcatta ttgatcgtct gagagatgtt gcgaaggctg ccgccacccc ggcattgcca
     1081 attcttgttg ttcttctgta tgacaaagaa ggtgctctcg gacaggcgtt ggcagaaatc
     1141 gcggtgctcg atgagtctgt tggcgcagaa gaacgggccc ggtttggaaa tctgataccg
     1201 gcacatagag agaagatgtg ccagatc
//
LOCUS       DAOQUC010001429         1226 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_163075_length_1226_cov_2.839453, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001429 DAOQUC010000000
VERSION     DAOQUC010001429.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1226
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_163075_length_1226_cov_2.839453"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..524
                     /locus_tag="PKH07_18860"
     CDS             <1..524
                     /locus_tag="PKH07_18860"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNT37059.1"
                     /translation="SGELKAPQWLLSTQNLLGVDIEHVRLTLKQWGVGARDAIIIVGV
                     WTAIGGNNMLLYLAALSNVPQELHEAAQIDGAGRCAAFWNVTWPQLAPTTFFILVMSC
                     IGGLQGGFEQARVMTAGGPAGTTTTLSYYIYRKAFEEFEIGYASAVSWILFVLILVVT
                     LVNWRFGSKEVSY"
     gene            521..>1226
                     /locus_tag="PKH07_18865"
     CDS             521..>1226
                     /locus_tag="PKH07_18865"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HNT37060.1"
                     /translation="MKRGNLASHLFLGFLALTMLLPFLWMVLASFKSLSEVEQLNPFP
                     TVWKLSNYPDVFEQVPFARYYFNSVFIALWVTFLQVLTSSLAAYSFARLRWRGRDVVF
                     RLYLATLMIPGVVTIIPNYALMVKLHLLDSYIGLIVPAAFTAFGTFLLRQFMLTIHPS
                     LDESASIDGATHWQIFWDIIMPLARPGIITLAILTFMGTYGSFLWPLIMIKSEHLRTL
                     PVGMLYFDTIYGRQTNL"
BASE COUNT          249 a          308 c          330 g          339 t
ORIGIN      
        1 gatctgggga gctcaaggcg ccgcaatggc tgctctcgac tcagaacctc ttgggagtgg
       61 atattgagca cgttcgtttg accctgaaac aatggggtgt tggtgcgcga gacgcgatca
      121 tcatcgtggg agtctggact gccatcggtg gcaacaatat gctcctgtat cttgcagcgt
      181 tgagcaatgt tcctcaggaa ctccacgaag cggcgcagat tgatggggca gggagatgtg
      241 cagcattttg gaatgtgact tggcctcagt tggcgccgac gacattcttc attcttgtga
      301 tgagttgcat cggtggactt caggggggat tcgagcaagc tcgggtcatg acggcgggcg
      361 gtccagcagg aacgacaacc acgctcagct actacatcta ccgaaaagca tttgaggagt
      421 tcgaaatcgg ctatgcctca gcggtttcat ggattctgtt tgtgctgatt cttgtcgtga
      481 cgcttgtcaa ctggcgcttc ggcagcaagg aggtcagcta ttgaagaggg gaaatcttgc
      541 cagtcatctg ttcttgggct ttttggcgct gaccatgctg ttgcctttcc tctggatggt
      601 gctggcaagt ttcaagtctc tcagtgaagt cgagcaactc aacccatttc caactgtctg
      661 gaaactgagc aattatcctg acgtgtttga gcaggtccct tttgcgcggt attatttcaa
      721 cagcgtcttc atcgcgctgt gggtgacatt tctgcaagtg ttgaccagct cattggcggc
      781 atattccttc gcgcgtcttc gatggagagg gcgcgatgtt gtctttcggc tctatctggc
      841 aacgcttatg attccaggtg ttgtgacgat cattcccaac tacgccctta tggtcaaact
      901 gcatcttctt gattcctata tcggtttgat cgttcctgct gcttttaccg cgttcgggac
      961 atttctactc aggcagttca tgctgacgat tcatccatca ctggacgagt ccgcctcgat
     1021 agatggcgcc actcactggc agatattctg ggatatcatc atgcccctgg cgagacccgg
     1081 gatcattacg cttgcgattc taacatttat ggggacatac ggatccttcc tctggcctct
     1141 catcatgatc aagagcgaac acctgcgaac gcttccggtc ggtatgctgt atttcgacac
     1201 aatctacggc agacagacca accttc
//
LOCUS       DAOQUC010001430         1224 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_163538_length_1224_cov_2.497861, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001430 DAOQUC010000000
VERSION     DAOQUC010001430.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1224)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1224)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1224
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_163538_length_1224_cov_2.497861"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1095)
                     /locus_tag="PKH07_18870"
     CDS             complement(<1..1095)
                     /locus_tag="PKH07_18870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37061.1"
                     /translation="MPKPFPSITPKTSAKANEDRNPGIQLLGRRFFSDQTVPELLLEM
                     LLVATSAKRIGDRNLPSDTLLPERDVLLGWPEGAVLEYAPKARLNLKMFAFLSASKLE
                     TRHESHRQHYRNLIQMMGDSSKMSVSGGVESGEVIKTLENLFLGFQSVGGQRTWCAAS
                     FLPVARALVAAESIWRQTRSLTSAVKEWSDALPLFSQTQHLFLARGGELLYLQLCNAL
                     RQDAEVLKKWSRDADLSLSEREGDPVQLNLALGKSLASALDACPETVGKLAEFIDAGI
                     ESETSRHTDFEGSQKKPRFAECGWCPEESWQEGALFAVELMRLCESVIDPIERLEMLQ
                     IACAMQLLRSLCAQSVRYTEHSKCSVPGCPL"
BASE COUNT          302 a          370 c          272 g          280 t
ORIGIN      
        1 caggggacac ccaggtaccg aacacttgct atgttccgta tagcgtacgc tctgagcgca
       61 gaggcttctc agcagttgca tggcgcaggc gatctgaagc atctccaggc gctcgatggg
      121 atcaatcacg gactcacata acctcatcaa ttccactgcg aacaaagccc cttcttgcca
      181 actctcttcc ggacaccatc cacattcggc aaatcgaggc ttcttctgac tcccctcaaa
      241 gtctgtgtgc ctcgaagtct cggattcgat tccggcatct atgaactcgg caagcttccc
      301 aactgtttct ggacaggcgt ccagtgctga agcaagagat ttccccaacg ccaggttcaa
      361 ctgaacggga tcaccttccc gctcagaaag actcaggtcg gcgtctcttg accacttctt
      421 caacacctcg gcatcctgtc gaagtgcatt acacaactgc aagtacaaaa gctctccacc
      481 acgcgcaaga aataggtgct gcgtttgaga gaatagagga agcgcatcgc tccattcctt
      541 gacagcagaa gtcaaagacc ttgtctgacg ccagatactc tccgccgcaa ccagagccct
      601 cgcaactggt aggaaactag ctgcacacca agttcgttgt cctccaacgc tttgaaaacc
      661 taagaacaga ttctcgagtg tcttgatcac ttcaccgctt tctactccac ctgaaacact
      721 catcttagat gagtcaccca tcatctggat caagttgcgg taatgctgac ggtgactttc
      781 atgccgcgtt tccaacttcg acgcgctcag aaaggcaaac atcttcagat tgagtctggc
      841 tttcggcgca tattcaagga cggctccttc cggccatccc agcagcacat ccctctcggg
      901 caacagagta tctgaaggca gattcctatc cccaattctc ttcgcagacg tcgcaaccaa
      961 cagcatctcg agcagcagct ccggaaccgt ctgatctgag aagaaacggc gtcccagaag
     1021 ctgaatccca ggattgcggt cctcgttagc ttttgctgac gtcttaggtg tgatcgacgg
     1081 aaagggctta ggcatcagtc acctccaagc gaccttctcg aacggcatac acctgccgtc
     1141 ggaaagtgtg attcgtcctc agtctgacca gcatgatgtc atcaccgagt ttgttcattg
     1201 aaaaccggat caactgaccc ttga
//
LOCUS       DAOQUC010001431         1223 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_163747_length_1223_cov_2.826199, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001431 DAOQUC010000000
VERSION     DAOQUC010001431.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1223
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_163747_length_1223_cov_2.826199"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..777)
                     /locus_tag="PKH07_18875"
     CDS             complement(<1..777)
                     /locus_tag="PKH07_18875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37062.1"
                     /translation="MTRSVGVAYLLFALLILVSASFVSAEDRIYQTESEAHAAALEAA
                     QLDLQKQIYKARVDSVTPMDIYLTTQWKLRDQVQDVMSEAIVAQESSAEEGQVVVILE
                     VPIDSIPLRIRGFYREYGLYVRGQGVALCPRTRTTMPDLDSSQFVWQDQILSAMGEGK
                     ARDDTSDTEGLQQAFHAARAEAMRNLGAMMESLSLSGDRTLRDLISTNQAVRYRVHSL
                     IGRATPTKVDFVSEDVCQVTLELPLSLLPAALAVESASEEA"
     gene            complement(848..>1223)
                     /locus_tag="PKH07_18880"
     CDS             complement(848..>1223)
                     /locus_tag="PKH07_18880"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007504125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNT37063.1"
                     /translation="VENFCGLAKKGFYNGLTFHRVIRGFVAQGGCPKGDGTGDPGYKI
                     PSEFNSRKHVRGTVAMARSQDPDSAGCQFYICFGAIPHLDNQYTVFGQVVRGMEVVDK
                     FAVTEPGRGSVGDKMKTIRIIE"
BASE COUNT          298 a          357 c          307 g          261 t
ORIGIN      
        1 ggcttcctca ctggcagact caacagccaa tgctgcagga agaagggaca ggggcagttc
       61 gagcgtgacc tgacagacat cctctgacac aaaatccact ttcgtcggag tcgccctgcc
      121 gatcaatgag tgaactctgt aacggactgc ctggttggtg gatatcaaat cgcgcaacgt
      181 tcgatcgccg gaaagggaga gtgattccat catcgcgcca agattcctca tggcttctgc
      241 acgcgctgca tgaaaagcct gttggagtcc ctcagtatcg gaggtatcgt ccctggcctt
      301 accctcaccc atggcgctga gaatctgatc ctgccagaca aattgtgacg agtccagatc
      361 cggcatggtc gtcctggttc ggggacagag tgccaccccc tgaccacgaa catagagtcc
      421 atactcgcga tagaagcctc ggattctgag cggaatcgag tctatcggca cttcaagaat
      481 gacgacaacc tgaccctctt ccgccgaaga ctcctgcgca acgatcgctt cggacatgac
      541 atcctgaacc tgatccctca gtttccattg cgttgtgaga tagatgtcca tgggagtcac
      601 agaatccacg cgagctttat atatctgctt ctggagatcg agttgtgccg cttccaaagc
      661 agcggcatgc gcctcagatt ccgtctgata gatcctgtct tcagcgctga cgaacgaagc
      721 ggagacaagg atcaacagag cgaacagaag ataagcgaca cccaccgaac gcgtcatcgg
      781 atcgaaacct ttcccgggct catctcccga gctcccgcaa acaggcttaa gtcgtacgct
      841 gcttacgcta ttcgatgatt cggatcgttt tcatcttgtc accgacggaa ccgcgccctg
      901 gctcggtaac agcaaacttg tccacaactt ccatcccacg gacgacctgg ccgaacacgg
      961 tatactggtt gtcaagatgc ggaatcgcgc cgaagcagat gtaaaactga catccggcgg
     1021 aatcaggatc ctgcgatcgc gccattgcca cggttccccg aacgtgcttg cggctgttga
     1081 attccgaagg gattttgtag ccgggatcgc ctgtgccatc ccctttcggg caaccgcctt
     1141 gagcaacaaa tccacgaatc actcgatgaa aggtcaagcc attgtagaaa cctttcttgg
     1201 caagaccaca gaaattctcg aca
//
LOCUS       DAOQUC010001432         1222 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_163958_length_1222_cov_2.765210, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001432 DAOQUC010000000
VERSION     DAOQUC010001432.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1222
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_163958_length_1222_cov_2.765210"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(421..1222)
                     /locus_tag="PKH07_18885"
                     /pseudo
     CDS             complement(421..1222)
                     /locus_tag="PKH07_18885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011743985.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
BASE COUNT          249 a          350 c          310 g          313 t
ORIGIN      
        1 tcccgaacct tcccaggatt tgtcgtcgct acgagaattg cctttgaaca aaccacttca
       61 cgaacctctg agtagaatca tactggacgg cgaagttcta ctgccctgtt cagcaatgct
      121 gcagccagca actccaattc gtctggctca ccctctatgt gaaggctctt tgcggccgga
      181 tgggctctgc gccacaagaa tctcccggct gcctcggagc tggtcaagtt cttctgcact
      241 gaggtcaatc aggtgaggcc cttcgctgtt tctgtatttg atctcgattc tcatttggct
      301 ctccctatcg catagacctg tatcacaccc gatagcattt cagaaccggc gagggaattg
      361 tccctccgcc gtgttccgga ctgccgcgca gcgagcccgc catcatttcg atgccaggct
      421 gattctcgat ccaaactgaa gcgtcacagg gtcgatcttc ttgttgacct gtaccgggtc
      481 tctgaaaacg tacggaagac gattcaccct ctggcggaag tcaacatggt cggcgtcttg
      541 ctttgtgaac tcgaaattgg catctgcaaa caggggtttc gcatcaatcc ccactttcct
      601 gcggatgact ccaatgaatt ccgggttgat ttcgtaccca actgagttcc tgccgaggtt
      661 tcgcgccgcc agacatgtcg tcccacttcc cagaaacgga tcgagaacga catcttcaac
      721 aaaactgaac atccggatca accgcctggg gatttcttcc gggaacatag ccaggtggtc
      781 gtcttgtttc tcgccgggga tattccagtg tccggcgaag aactggttcc attcctcggt
      841 ggtcatccgc gattgttctt tgatctcctt gctgacggcc ggggccgttc cctgtttctt
      901 gaagatcagg atgaactcgt agtccagctt gaggattccg ttccgtggat acgggaatga
      961 gcccatgatc gtggcgccgc cggtggtgtt gcaggtggtt accttctgcc agatcacggc
     1021 acccatgtag tcgaagccca tcgtctcaca gaacttgata atctccgtgc ggatgggaat
     1081 caccttgtat cgtccgtaat agaccgaccg ggcgaactgg tcgccgatgt tcacacacag
     1141 ccggcaaccc ttggtcaata ctctgtggca ctccgaccag acgagattga ggttgttgat
     1201 ataggtctcg tagctgtcat tg
//
LOCUS       DAOQUC010001433         1222 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_164021_length_1222_cov_2.347044, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001433 DAOQUC010000000
VERSION     DAOQUC010001433.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1222
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_164021_length_1222_cov_2.347044"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /locus_tag="PKH07_18890"
     CDS             <1..384
                     /locus_tag="PKH07_18890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37064.1"
                     /translation="SLILALGCSLVGTGCFYTRVKDRAYFSYCYCGATEVDARTVLEH
                     ADRLARLNGLDSPRFFLPWKHSADPRVWSVQGHSKDTPFLVSVAFKDAIEVVLEIHAG
                     RTPKSWTKITKSIEDFQRENWTLKQ"
     gene            complement(603..>1222)
                     /locus_tag="PKH07_18895"
     CDS             complement(603..>1222)
                     /locus_tag="PKH07_18895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37065.1"
                     /translation="DDGDSGYSTTGTWGYSTGSSQAYNNDYNWASTQASETLSARWTP
                     TFPASGQYDVYVRYQDGSNRASNAPYTVYYSGGSQSFSIDQRTGGGTWVLLGRFAFDA
                     GSTGYVKLSNNTGATGSVVIADAVRFTPVVSTPTNTPTRTPTVPTSTPTQTPTRTPTW
                     TPSRTPTWTPTRTPTRTPTPQAATSTPVATATPHTSSANLWYLYE"
BASE COUNT          267 a          324 c          339 g          292 t
ORIGIN      
        1 tcactcatct tagccctagg gtgttctctt gttggaacag gctgcttcta cacccgtgtg
       61 aaggatagag catatttcag ctactgttat tgtggcgcaa cagaagtcga cgctcgaacc
      121 gtgcttgagc atgcggatag gctggcacga cttaacggac tggactctcc tcggtttttc
      181 ctgccgtgga agcatagtgc cgacccacgg gtatggtcag tacaagggca ctccaaagac
      241 accccatttc tcgtcagtgt cgcttttaag gacgccattg aagtcgtact agagatacat
      301 gcaggtcgca cacccaaatc atggacgaag ataaccaaat ctatcgagga ttttcagcga
      361 gagaattgga cactgaaaca gtaactatct tgtgctgggc ggagcgtgtc gcccggtttc
      421 ccccgaaacc ggcatcctgg gctgagggca acccagtcca cttcctgcac agaacaagag
      481 atgggtctgg tgatcaatcc tacgcagctg aaagatcgaa gcataaatgc tgctctaccg
      541 ccggcaaaga acaacgaccc ctcctgttcg agggcgccgc agtcccatag aatgattcag
      601 cctcactcat agagatacca gagattcgcg ctactggtat gcggtgttgc tgtggcaaca
      661 ggtgttgatg ttgccgcctg tggcgttggt gttcgtgttg gcgtacgtgt tggtgtccac
      721 gtcggcgttc gactcggagt ccaggtcggt gttcgcgttg gtgtttgtgt cggtgtactg
      781 gtcggaacag tcggtgttcg agtcggtgtg ttagttggag tcgagacgac cggagtgaaa
      841 cgcacggcat cagcgatgac aaccgaacca gtggctcctg tgttgttgct cagtttcacg
      901 tagccggtgc ttccggcatc aaacgcaaat ctccccagta acacccaagt tcccccgccc
      961 gtcctttgat cgatactgaa cgactgagat ccaccactgt agtagacagt atagggagcg
     1021 ttgctcgctc ggttcgaacc atcctgatat cgaacataga catcgtactg acccgaggcg
     1081 gggaaggttg gagtccagcg cgcactgagc gtttccgacg cctgagtgct tgcccagttg
     1141 tagtcgttgt tatatgcctg ggaacttccc gttgagtagc cccaagtccc cgtggtcgaa
     1201 tatcccgaat ccccgtcgtc aa
//
LOCUS       DAOQUC010001434         1221 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_164188_length_1221_cov_2.937393, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001434 DAOQUC010000000
VERSION     DAOQUC010001434.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1221
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_164188_length_1221_cov_2.937393"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..864
                     /locus_tag="PKH07_18900"
     CDS             <1..864
                     /locus_tag="PKH07_18900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37066.1"
                     /translation="NTPTFTPGAPYEVIVDDGDVWYSDGGNWGYTNDGNAESYNGDYL
                     WKSTTETETNWAKWQPYLNGTNYDVYVRYRAGSNRVTDARYTVTFAGGATQSIAVDQT
                     VNGGVWVFIGTFRFDTGVNGSVVLSNQSSQVGGRVVIADAVRFVLTPPVTRTIDDGDA
                     GFSLNGAWTYVTNGGLEPINGDYYYASTASSETKSAIWRPDLVQDGYYRVSVRYRQGA
                     NRSNQAPYTVYYNGGWQTISVNQQVNGGTWFVLGTWHFKSGTSGYVKLGNGPAPTGKI
                     VLADAVLFEPQ"
BASE COUNT          296 a          267 c          332 g          326 t
ORIGIN      
        1 aatactccaa ccttcactcc cggagcgcct tatgaggtaa ttgttgacga tggagatgtt
       61 tggtattccg acggaggcaa ttggggctat acaaatgacg ggaatgcaga gtcttacaat
      121 ggtgactatc tctggaaaag cactacggaa actgagacga actgggctaa gtggcaaccc
      181 tatctgaatg gcaccaacta tgacgtctat gtcaggtacc gcgctggctc aaatcgcgtt
      241 acggacgctc gttacacggt tacatttgcc ggcggagcca cacagagcat cgccgtcgat
      301 cagactgtca acgggggtgt ttgggttttc atcggaacat tccgtttcga tactggagtg
      361 aacggttctg tcgtgcttag caaccagtcg tcacaggtcg gcggaagagt tgttatcgct
      421 gacgcggttc ggtttgtgtt gacaccacct gtcacaagaa caatcgacga tggcgatgct
      481 ggcttcagtc tcaacggggc ttggacatat gtcactaatg gtggtttaga accaatcaat
      541 ggtgactatt actatgcttc gaccgcctca tcggaaacca agtcggccat ttggaggccg
      601 gatctcgttc aggacggata ctaccgcgtc tccgtcaggt atagacaagg tgctaaccgt
      661 tcgaatcagg caccgtatac cgtctactac aatggtggtt ggcagactat ctctgtcaat
      721 cagcaggtca atggtgggac atggtttgtt ctcggcactt ggcacttcaa gagtgggaca
      781 agtggttatg tcaaacttgg caatggtccc gctccgacgg gtaagatcgt tctggcggat
      841 gctgttctgt tcgaacctca gtaggagctg cgtgccttct ggagtgtgct tctgtctttc
      901 agtatcttgt ttggggctga tcgagaatca gccccttgtt cttagtagtt ctgacaagac
      961 tcatcagtga gaatgcttgg tgtctgcttt tgtcggattc ctgcaatctc aatgagatcc
     1021 aggaagtgga acctaactga ggagaaaatc agcagatcat tgacagaaga tacctgtttg
     1081 agagaatata caaaagttct tacagacggt atctgtttgg caaaggcaga ggcaataccg
     1141 agtagttaaa catttttcag aaaggggtga tctgagtgaa gaagaacttg gtcgttctga
     1201 ttatcctgct gattgtgctt g
//
LOCUS       DAOQUC010001435         1221 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_164195_length_1221_cov_2.810463, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001435 DAOQUC010000000
VERSION     DAOQUC010001435.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1221
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_164195_length_1221_cov_2.810463"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(210..>1221)
                     /locus_tag="PKH07_18905"
     CDS             complement(210..>1221)
                     /locus_tag="PKH07_18905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37067.1"
                     /translation="DFPSALPWLEQLIGQIAESVENAWDTRRPAVLKGMVGKAPGISW
                     CRRIITRDGRIVPYRNRPPDDEVLKEPRDENVPVVLVEGDGWNGVLVNFACHATTVQV
                     QPLVSADFPGVACRLVEETLGAEACLFFQGTCGNVNPLRGTSCFDDVDLYGQALADEV
                     LRCVRQLQQQSSSPMPSLVNVVRETVALERRTLPDRATLERAVAEGKKAIDAARTLEE
                     KTEAIAAYREAAEPLRLVQLGSGPVFCEVQVIRLGDILIVGIEGEVFVEYGLRIHAKS
                     PAPITLVCAYSNGYQGYIVTPETYEEGGYEVSLGPWSRVTRNAGDVLIGRIEELIKTV
                     SS"
BASE COUNT          270 a          346 c          341 g          264 t
ORIGIN      
        1 gtcaccgagg ttcctgaaga ggagcctccg atgccagccg gtcctggtgg cggaatgcct
       61 ggtggaatgc ccggtggcat gggtggaatg ggcggcatgg acgggatgta ctgagccgtc
      121 ggaaaccgtc tggatcgaag acggcgttgt gcaacagata cggaatacag ggtgtcaatc
      181 ggattcggtt ggcacccttt tacttctact tatgaagaga ccgtcttgat gagttcttcg
      241 atacggccaa tcagcacgtc gcctgcattg cgggtaactc tcgaccatgg gccaagggaa
      301 acctcatagc ccccttcctc ataggtctca ggggtgacga tgtagccttg atagccattg
      361 ctgtaggcgc agaccagagt gattggtgcg ggtgattttg catggatgcg cagtccatac
      421 tcgacaaaga cttctccctc aataccgaca atcagaatgt ctcccaggcg aatgacctgc
      481 acttcacaaa aaacggggcc actgcccaac tgcaccaaac gcaggggttc ggccgcttct
      541 cgatacgcgg cgatggcctc ggtcttttct tcgagcgtcc gagcagcgtc aatggccttc
      601 ttgccttcgg cgactgcgcg ctccagagtt gcccgatcgg ggagcgttct tctctcaaga
      661 gcaaccgtct ctcttacaac attcaccaag gatggcatcg gagaagatga ctgctgttgc
      721 agttgtctga cgcatcgcaa cacttcgtcg gcaagtgcct ggccgtagag atccacatcg
      781 tcaaagcagc tggtgccacg aagtggattc acatttccac aggttccttg aaagaagaga
      841 cacgcctcag ctcccaaagt ctcttcgacc aaacggcagg ccacacccgg gaagtctgcg
      901 gataccagtg gctgaacctg aaccgttgtg gcgtgacagg caaaattcac aagaacccca
      961 ttccagccgt cgccttcgac caagacgaca ggaacattct catcccgagg ttctttgagc
     1021 acctcgtcgt caggcggccg atttcggtaa ggaacaattc tgccgtcgcg agtgataatt
     1081 cttcggcacc atgagatccc tggcgctttg ccgaccatgc ctttgagcac cgctggccgg
     1141 cgagtgtccc atgcattctc gacagattcg gcgatctgtc cgatgagttg ttcgagccac
     1201 gggagcgcgg aagggaaatc c
//
LOCUS       DAOQUC010001436         1217 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_165095_length_1217_cov_2.719449, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001436 DAOQUC010000000
VERSION     DAOQUC010001436.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1217
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_165095_length_1217_cov_2.719449"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(109..564)
                     /locus_tag="PKH07_18910"
     CDS             complement(109..564)
                     /locus_tag="PKH07_18910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37068.1"
                     /translation="MKDNSSDLESVQMEMQPGVLTLEGFLGTDSRSLAEIVQADDAEV
                     QRLGLTHERIAERLTQLTQMALQGFGTPQKGDEFSVVCSEAIGGWVRCPFRDKGRFRK
                     GEIVLTDKNSGRMLTWTPLSVHLIEEHGFYQGRGSHYRLEPSLLKEMLF"
     gene            complement(573..>1217)
                     /locus_tag="PKH07_18915"
     CDS             complement(573..>1217)
                     /locus_tag="PKH07_18915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNT37069.1"
                     /translation="QGAEPVRFKAGQYAQFRIPSYGASPEPVYRAYSISSAPTDDRAL
                     EFVIRRVPSGIATTYIFDHLKRGDVLSVNGPYGDFYLRDTDAEIVFIAGSTGIAPIKS
                     ILHTMTEQGINRKARFFFGAVQKRDLFYREEMAEFERNLPNFHYIPALSRKLPEDQWD
                     GEEGLITEVLERHLTDGPNVEAYLCGSPGMIDACVKVLMTKGVTADRVFYDKFA"
BASE COUNT          289 a          343 c          300 g          285 t
ORIGIN      
        1 cccccttgat ccccccttgt taaggggggc aggcttgttc ttacccccct tcggtaaggc
       61 aggggggatt ttcggagaga gcatgtgtct ctctccagaa gcatatcctc aaaacaacat
      121 ctccttcaag agggacggct caagccgata gtgtgatcct cttccctggt agaaaccatg
      181 ctcttctatg agatgcaccg acagtggtgt ccatgtcaac atcctgccac tgttcttatc
      241 agtcagaaca atttcccctt tgcggaaacg tcctttgtct cggaacggac agcgcaccca
      301 accgcctatg gcttcgctgc acacgaccga gaactcatcg cctttctgcg gagtaccaaa
      361 tccctggagt gccatctggg tcaactgcgt aagtctttca gcgatccgct cgtgtgtgag
      421 tccgagtctt tggacttctg catcatctgc ctgcactatc tccgcaagag agcgcgaatc
      481 ggttcccaga aagccttcca gagtaagcac tccaggctgc atctccatct gtacggattc
      541 aaggtctgac gaattatctt tcatgatgca cctcaggcaa acttgtcata gaaaacgcga
      601 tccgcggtaa caccctttgt catcaggact ttgacacaag catcaatcat ccctggactt
      661 ccacacagat aggcttccac attgggacca tcggtcagat gacgttccag aacctctgtg
      721 atcagaccct cttcaccgtc ccactgatcc tcaggcagct tgcgggaaag cgcagggatg
      781 taatggaaat tcggaagatt cctttcgaac tcggccatct cctcacgata gaacaagtcc
      841 cgcttctgga cagcgccaaa gaaaaagcgc gccttgcggt tgatcccctg ttcggtcatc
      901 gtgtgcagaa tggacttgat gggagcaatt cccgtcgagc cggcaatgaa gacgatctcg
      961 gcatcggtgt ccctgagata gaaatctcca tagggtccat tgactgaaag aacgtctccc
     1021 cgtttgagat ggtcgaagat gtaagtagtg gcaatgcctg aagggacacg ccgaatgaca
     1081 aattccaaag cccgatcgtc tgtgggtgcg cttgaaatcg aataggcgcg gtagacaggt
     1141 tcaggactgg caccgtaaga gggaattcgg aattgtgcat actgtcccgc tttgaaccga
     1201 acgggctcag ccccctg
//
LOCUS       DAOQUC010001437         1216 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_165304_length_1216_cov_2.764858, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001437 DAOQUC010000000
VERSION     DAOQUC010001437.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1216
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_165304_length_1216_cov_2.764858"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..812
                     /locus_tag="PKH07_18920"
     CDS             <1..812
                     /locus_tag="PKH07_18920"
                     /inference="COORDINATES: protein motif:HMM:NF019297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37070.1"
                     /translation="TFSHGDAADVETTALAAIAFLRSGKYPETTGKALTYLIQKKGAQ
                     GHWGSTQATILALKALMLSLGSRTEEVDASIVVSLNDETISELKVTPEDSDVMRLVDL
                     GEKTKEGVSNVKLSLKGEGSLLYQIVGRYYLPWTQKAAPEEPMTITVNYDRTELAVND
                     IAAVNVKVKNNRPVAAQMIIVDVGTPPGFQVQAADLESLVEKKVFSKYEMTPRQVIAY
                     FEKIEGNQTVEFSYTIQAKFPLRAKTPQSKVYEYYNPEVSNIAAPLDLKVE"
     gene            1018..>1216
                     /locus_tag="PKH07_18925"
     CDS             1018..>1216
                     /locus_tag="PKH07_18925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37071.1"
                     /translation="MNGVSPLRGFGGQPERYYRCPQCLSIVLVPLPTGRKLNAFFEES
                     EGVQSLIESESKRRTYFNHRLN"
BASE COUNT          303 a          302 c          350 g          261 t
ORIGIN      
        1 tgaccttctc acacggagat gcggcggatg tggaaaccac agcacttgcg gcgatcgcct
       61 ttctgaggtc gggcaaatac ccggaaacca ccggcaaagc gctgacgtac ctcatccaga
      121 agaagggagc acaaggacat tggggttcaa ctcaggcgac aattctcgct ctgaaggcct
      181 tgatgctgtc gcttggcagc agaaccgaag aggtggacgc ctccattgtg gtctccctga
      241 acgatgaaac gatttcggaa ctgaaggtga caccagagga ttccgatgtg atgcgactgg
      301 tggatcttgg cgagaagacc aaggaaggcg taagcaacgt gaaactgagc ctgaagggcg
      361 aggggagcct gctctatcag atcgtcggac gatactacct tccctggaca cagaaagccg
      421 cgccggaaga gccgatgacc attacggtca actatgatcg gacggaactg gcggtgaacg
      481 acattgcagc ggtcaacgtc aaggtcaaga acaacagacc ggtcgccgca cagatgatca
      541 ttgtggatgt tggaacgcct ccaggatttc aggttcaggc ggcggacttg gagtctcttg
      601 ttgagaagaa ggtgttcagc aagtatgaga tgactccacg acaggtgatc gcgtactttg
      661 aaaagatcga agggaatcag acggtcgagt tttcctacac gatccaggca aaattcccac
      721 ttcgtgcaaa gacgccgcag tccaaggtgt atgagtacta caatccggaa gtctccaaca
      781 ttgcagcacc tcttgatctg aaggtggagt agactggatg ctatccttgg gagcgccgag
      841 tacttgctcg gcgctcccaa cggtgtgttg ggcactgagg cgagatacct tgcgatgcct
      901 atcagtctcc tgccggagaa gttgaacccg aatctcctgt cgaagtctcc attgtgtacg
      961 aatccgtgac aactatggta gtttgttctc atgaactgca ctatctgcgg agcaagtttg
     1021 aacggtgttt ctcccttgag gggcttcggg gggcaacccg aacgttacta ccgatgtccg
     1081 caatgtctgt ctatcgtgct cgttccgctg ccaacgggaa ggaaactcaa cgcgttcttt
     1141 gaagagtctg agggtgtcca atcgttgatt gagtcggaga gcaagcggag aacctacttc
     1201 aatcatcggc tcaacc
//
LOCUS       DAOQUC010001438         1216 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_165424_length_1216_cov_1.784668, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001438 DAOQUC010000000
VERSION     DAOQUC010001438.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1216
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_165424_length_1216_cov_1.784668"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(183..>1216)
                     /locus_tag="PKH07_18930"
     CDS             complement(183..>1216)
                     /locus_tag="PKH07_18930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37072.1"
                     /translation="MWASFWEESNSRPLVAIVRPKDGVEPSPAPATYEMTRGDLDLVL
                     DKIVQWASTREFLADAIPCFCVSFAADHFAALLGGDFKWHPDSAETFWPVPIIEDWSE
                     VNIQFRRDNPWWQRTVETIRRCRQRLDGRMIVSGCHLQGGLDCLAALRGVNRLLLDLL
                     DHPEEVAKALQQIDDALEQVRDALDDEFGVKTFGSMTRHGLYSPGSSDVPQCDFSAML
                     SPDMFQQFGLPSLKRQCEQLDHSTYHLDGQEAIKHLEAVCSIEKLGVIQWQPGAGAAQ
                     TKDWSFLRERIDSLGKGQILGGSHETIKHLWQTFRSRRLFFGARAESRDDAERFLEEL
                     ERLEKPAGR"
BASE COUNT          290 a          362 c          312 g          252 t
ORIGIN      
        1 ggatcctggg ttgggggcaa cccaggctac gggcagcaat ccacaaaagg gcgaatcatg
       61 atccgcccct acaaagacaa ctacgtagtt attcaggagt gcggtagctt tctaccgctg
      121 agcacctttc acgaaaccaa ggctctgcac tcgcttctac tggagtagcg tagactatca
      181 tgtcagcgac ccgctggctt ctccaaccgc tcgagttcct cgagaaatct ttcggcatca
      241 tccctggatt cagcgcgagc accgaagaac agtcttcgag aacgaaatgt ctgccagaga
      301 tgcttgatcg tttcgtggct gccaccgaga atctgtccct ttcccaggga gtcaattctc
      361 tctcgcagaa acgaccagtc cttggtttgt gctgcgcccg ctcctggctg ccactggatc
      421 acgcccaatt tctcgatgga gcagacggct tcgagatgtt tgatcgcctc ttgcccgtcg
      481 agatggtagg ttgaatggtc aagctgttcg cactgccgtt tcagggaagg cagaccgaac
      541 tgctgaaaca tgtcggggct gagcatggcc gagaagtcac actgaggaac atcagaactt
      601 cctggagaat aaagcccgtg acgggtcatg ctgccaaaag tcttcacccc gaactcatca
      661 tccaacgcat cccgtacttg ctcgagcgca tcgtctatct gctgcaatgc ctttgcgacc
      721 tcctccggat gatcaagcaa atccaggaga agtctgttga caccgcgcag cgccgccaga
      781 cagtccaagc caccttgaag atgacagcct gaaacgatca tcctgccgtc gagcctctgc
      841 cgacagcgcc gaatggtctc cacggtccgc tgccaccagg gattgtctct gcggaactga
      901 atgttgacct cactccagtc ctcaatgatc ggaacaggcc aaaaagtctc cgcactgtca
      961 ggatgccatt tgaagtcgcc ccccaacaat gccgcaaaat gatcggctgc gaaggagacg
     1021 cagaaacagg gaatggcatc agcaagaaac tctcgagtcg aagcccactg aactatcttg
     1081 tcgagcacca agtccagatc tcctcgcgtc atctcgtagg tggcgggagc aggggaaggt
     1141 tccacaccgt ctttgggacg cacgatggcg acgagcggtc gggaattact ctcttcccaa
     1201 aacgaagccc acatct
//
LOCUS       DAOQUC010001439         1215 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_165496_length_1215_cov_3.795690, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001439 DAOQUC010000000
VERSION     DAOQUC010001439.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_165496_length_1215_cov_3.795690"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="PKH07_18935"
     CDS             <1..249
                     /locus_tag="PKH07_18935"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HNT37073.1"
                     /translation="YIGFKIAIGCRDLSGRLAAAGIITCFGLQAVMHLFVVTTLMPIT
                     GVALPLMSYGGSSLITILAGMGILMSIARYTGHFELEA"
     gene            258..>1215
                     /gene="murG"
                     /locus_tag="PKH07_18940"
     CDS             258..>1215
                     /gene="murG"
                     /locus_tag="PKH07_18940"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HNT37074.1"
                     /translation="MRLAIAAGGTGGHIFPAVALIEGLLARKDSAALVFGRSTGMERN
                     ILGNRHDIEYVGLQIRPFLRSRLTVWPIALAELVQSVFFACRTLRQWKPDAVLGMGGY
                     VSAPVVMAALLTRLPVAVADQNALPGRANRFLGRFATRVFCGCEESLPFFSKRKRRLT
                     GIPLRVGATMACERPPFETFGLKQGLFTLLVFGGSQGALALCEAVRQMLPVLDQHGVP
                     YQILLQVGARNSEWIENAQMPNHVKTVEMIEDMSAAYACADAVVCRAGAVSLAEMAVQ
                     GKPAILIPYPFAKDDHQTKNAKAWGDVGAARVIEQSLLTPESL"
BASE COUNT          248 a          302 c          376 g          289 t
ORIGIN      
        1 tatatcggat tcaagatcgc catcggatgt cgtgatctgt ctgggaggct ggcagcggca
       61 ggaatcatca cctgttttgg tcttcaggca gtcatgcatc tttttgtggt cacaacattg
      121 atgccaataa cgggggttgc tctgcccctg atgtcttacg gaggatcttc tctgatcacg
      181 attcttgcag gcatggggat tctgatgagt atcgcccgat atacgggaca ctttgagttg
      241 gaggcgtagg cgacacgatg cggttggcga tagcggcggg tggcacaggc gggcacatct
      301 ttccggcggt ggctctcatt gaggggctgc tcgcgcggaa ggactctgcc gcgttggtgt
      361 ttggtagatc cacgggaatg gaacgaaaca ttctcgggaa ccgccacgac attgaatacg
      421 tcggtctgca gatcaggcct ttcctgagaa gccgcctgac agtttggcca atcgctcttg
      481 cggaacttgt gcagtccgtt ttcttcgcat gcaggactct ccgacagtgg aaacccgatg
      541 ccgtgttggg aatggggggg tatgtctctg caccggtggt catggcagca ctgttgacga
      601 ggcttcctgt agcggtcgca gatcagaatg ctcttccagg tcgggcaaat cggtttcttg
      661 gccgctttgc cactcgggtc ttctgcggtt gtgaggaaag ccttccgttc tttagcaaac
      721 gaaagagacg cttgacgggc atacctttgc gcgtcggggc aaccatggcc tgcgagagac
      781 caccgttcga aacgtttggt ctgaagcagg ggcttttcac cctgttggtg ttcggaggca
      841 gtcaaggtgc tctggcgctc tgtgaggcgg tcagacagat gctgcctgtt ctcgatcaac
      901 atggagtgcc gtaccagatt ctccttcagg ttggagcaag aaactccgag tggatcgaaa
      961 acgcgcagat gcccaatcac gtaaaaacag tcgagatgat tgaggatatg tcggctgcct
     1021 atgcgtgcgc cgacgcggtc gtgtgccgcg caggagcagt ctcgctggca gagatggcag
     1081 ttcagggcaa gcccgcgatt ctgattccct acccttttgc caaagacgat catcagacga
     1141 agaatgcaaa agcatgggga gatgttggcg cagcgcgtgt tatcgaacag agcctactca
     1201 cgccagaaag tctcg
//
LOCUS       DAOQUC010001440         1215 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_165557_length_1215_cov_2.719828, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001440 DAOQUC010000000
VERSION     DAOQUC010001440.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_165557_length_1215_cov_2.719828"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1215)
                     /locus_tag="PKH07_18945"
     CDS             complement(<1..>1215)
                     /locus_tag="PKH07_18945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37075.1"
                     /translation="EYDLVVVCLVENRIENPFRAIANLVKHCMKIGGSILFVHEEGDT
                     SASPRRGAANRHAFLREFCLAMSMSSTGTYPSKPSVSSDSTLAHYLYTKHSETQNSWC
                     NLADWRSRMVSWNIEDVYEPGYTLERYNERLVNEITSLLKKTECSCTGSFDESPVRGI
                     YYIDTTRFLNGIADPFVKTAAADRSEILATVGGNEFTNLKIAVLPLIAAREGSSPIKM
                     RFEIDSVRKFLGTPEHIQITLIPLEPECSDLMVNSESQEYLLTNNCLRLLAADARSVF
                     GEMPMVLYFFLSEGVDIGTASVFLFCCNQWLSPCSFGAVFEAFYVYLWNLQAFLMHHN
                     AMLLQRANLLESTRASIERLRDLLNEAREPLANLNNLLDPASSLTFGGASVDIFYDPN
                     RQTVALSETIGT"
BASE COUNT          338 a          289 c          291 g          297 t
ORIGIN      
        1 ctgtcccaat agtctcactc aacgcaacgg tttgtcggtt tggatcatag aagatatcca
       61 ctgaagcacc accaaaggtc aatgaacttg cagggtcaag aagattgttc agatttgcca
      121 aaggttctct cgcttcattc aggagatcac ggagtcgttc tattgaagcg cgagtggatt
      181 cgaggagatt ggcgcgctgc agaagcattg cattatggtg catgagaaat gcttggagat
      241 tccataggta gacgtagaaa gcctcaaata cagcgccgaa actgcaagga gaaagccact
      301 ggttgcagca gaagagaaaa actgaagcag ttccaatgtc cactccctct gaaaggaaga
      361 agtacaagac cataggcatt tcgccaaaaa cacttctcgc atcagccgca agaagtctga
      421 ggcagttatt ggtaagcaaa tactcctggc tctcagaatt caccatcagg tcggagcatt
      481 ctggttcaag cggaattaga gtaatctgaa tgtgttccgg tgtgcccaga aacttcctga
      541 cggaatctat ctcgaacctc attttaattg gactactgcc ttcccgagcc gcgatgagag
      601 gaaggacagc aatcttcaaa tttgtgaact catttccgcc aacagtcgct aatatctccg
      661 agcgatcggc agcagctgtc ttcacaaagg gatcagcaat gccgttaaga aatcgagttg
      721 tgtctatgta gtagattccc cttactggtg attcgtcaaa gctccctgta caggagcact
      781 ccgtcttctt cagcaaggaa gtaatctcat tgactaatct ctcattgtag cgctcaagag
      841 tgtatccagg ctcgtagaca tcttcgatat tccaactgac cattctggaa cgccagtcag
      901 caagattgca ccaagagttc tgggtttctg aatgcttcgt gtacaggtaa tgtgcaagcg
      961 ttgaatcgga ggacaccgag ggttttgacg gataggttcc cgtcgaactc atggacattg
     1021 cgagacagaa ctcccttaga aaagcatgac ggtttgcagc accccgcctt ggcgatgcac
     1081 tagtgtcccc ttcttcatgg acaaagagaa tgctgccacc aatcttcatg caatgcttga
     1141 cgagattcgc aatcgctcgg aagggattct caatacggtt ttcgacgaga caaacgacga
     1201 ctagatcgta ttcgc
//
LOCUS       DAOQUC010001441         1214 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_165713_length_1214_cov_3.855910, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001441 DAOQUC010000000
VERSION     DAOQUC010001441.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1214
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_165713_length_1214_cov_3.855910"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            39..680
                     /locus_tag="PKH07_18950"
     CDS             39..680
                     /locus_tag="PKH07_18950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37076.1"
                     /translation="MSRCALSSILTCFVFFGQSVCLRGASAQTITPHAETATPTPTRT
                     PFPFNPDLNGDLYVDEADLLLLRLAWHQYLGDATETASPTATCTMTPTPSATYTPTST
                     RTPTRTPTISPTPISLVGLWTGFGVSDGGVGNPSITMTVLDAETATVKLGGTFISTYS
                     FQPRVVGGSFDFSGYNGNYLVLQGTVQSLNSINGRYSLSGSVNDTGTFTVARQ"
     gene            787..>1214
                     /locus_tag="PKH07_18955"
     CDS             787..>1214
                     /locus_tag="PKH07_18955"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014828090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HNT37077.1"
                     /translation="MRIVIAPNAFKECLSPAQVSRAIERGVKRASASAKTVLVPLADG
                     GDGTVEALVTATQGRIVEALVSDPLGRSIQARYGILGNGETAVIEMAEASGLKRIASE
                     ERNPLITSTRGTGDLVLDAMRNGVRSIVLGIGGSATVDGGT"
BASE COUNT          273 a          308 c          324 g          309 t
ORIGIN      
        1 cttagcactg tatctcctgt gcagaaggag gaaacttgat gtctagatgt gctctgtcaa
       61 gtattttaac ttgctttgtc ttctttggtc agtctgtatg tttgcgaggc gcttctgccc
      121 agaccattac tccccatgct gagacggcta caccgacacc cactcgaact ccctttccat
      181 ttaaccctga tctaaatgga gatttgtacg ttgacgaagc ggatctactg ctgcttcgct
      241 tggcctggca ccagtattta ggggacgcta ctgaaaccgc atcgccaacc gcaacatgca
      301 ccatgacgcc aaccccatcg gcaacataca ccccaacttc tacccgaaca cccaccagaa
      361 cgcctacgat atccccgact cctatctcct tggtcgggct ttggacgggc tttggagtca
      421 gcgatggtgg ggttggaaac cccagcatca cgatgacggt tctggatgcg gagacggcga
      481 ccgtgaaact ggggggaaca tttatctcga cctactcttt tcagccaaga gttgtaggag
      541 gttccttcga tttctcgggt tacaatggta attaccttgt tttgcaggga acagtacaga
      601 gtctgaactc gataaatggg cgctatagcc tgagcggttc tgtcaatgac acaggcacat
      661 tcactgtggc aaggcaatag aaccagccgc tcacttgcgc tcccaggtgg taacctgctt
      721 gtgaaggtgc ttgcttccaa gaagaaacga cttttgtgga gtcagctttc ccaggaggta
      781 gaatagttgc gaattgtcat tgctcccaac gctttcaaag aatgtctgtc tccagcacag
      841 gtctctcggg caattgaaag gggtgtcaag cgggcttccg cttctgcaaa gaccgttctt
      901 gtccctttgg cagacggtgg agatggtact gttgaagcgc ttgtcactgc aacgcagggt
      961 cgaattgtgg aagcacttgt ctcagatccc ttgggcagga gcattcaggc acgctatggg
     1021 atccttggca atggtgagac ggccgtcatc gaaatggctg aggcttctgg tctgaagaga
     1081 attgcttctg aggaacggaa tcctctgatc acgagcactc gtggaactgg cgatttggta
     1141 cttgatgcga tgcgaaacgg tgttcgctca attgtcctcg gcattggtgg aagtgccaca
     1201 gtggacggtg ggac
//
LOCUS       DAOQUC010001442         1214 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_165764_length_1214_cov_2.729077, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001442 DAOQUC010000000
VERSION     DAOQUC010001442.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1214
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_165764_length_1214_cov_2.729077"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..400)
                     /locus_tag="PKH07_18960"
     CDS             complement(<1..400)
                     /locus_tag="PKH07_18960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020676442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNT37078.1"
                     /translation="MPVFVWEGKLANGNIKRGEIEADSKASVQLLLKRQKILPTKIKS
                     KPKQIVLFEQKIKTKEIVIFTRQFSTMINAGLPLVQCLDILSSQQSNPTFKKVLSQIK
                     TDVEGGSTFADALSKHPKIFDNLYVNLVAAG"
     gene            complement(417..>1214)
                     /locus_tag="PKH07_18965"
     CDS             complement(417..>1214)
                     /locus_tag="PKH07_18965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012469712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HNT37079.1"
                     /translation="IPFDIIPLETLGLPNVVGRLTTLPRGLVLVTGPTGSGKSTTLAS
                     FLDKINTERRDHIVTIEDPIEFVHKHKGCIVNQREVGSDTKNFTNALKYILRQDPDVV
                     FIGEMRDLETMQAALTIAETGHLVFATLHTNSAVQTVNRVVDAFPAHQQSQIRAQMSF
                     VLEAVISQQLLPRADGKGRVLAAELMISTPAIRNLIREDKIHQIYSQMQVGQGKHGMQ
                     TMNQALFALFTRKLISLDDAMGWSMDPEELRQMMASPNQSRGMSPNR"
BASE COUNT          237 a          314 c          356 g          307 t
ORIGIN      
        1 ctcctgcggc cacgaggttc acatacaggt tgtcgaagat cttcggatgc ttagacaaag
       61 cgtcggcgaa tgtgctgcct ccctccacat cggttttgat ctgggagagt actttcttga
      121 aagtgggatt tgactgctgg gacgagagaa tatcgagaca ctgcaccagg ggcaggcctg
      181 cattgatcat ggtcgagaac tgcctggtga agatgacgat ttccttggtc ttgattttct
      241 gctcgaagag aacgatctgc ttcggcttgg acttgatctt ggtgggcagt atcttctgcc
      301 gcttgagcag gagctgtact gatgccttgg aatcagcttc gatctctccc cttttgatgt
      361 ttccgttggc gagtttgcct tcccaaacaa aaaccggcat gatctatctc ctttccctag
      421 cggttggggg acatccctct ggactggttg ggggaggcca tcatctgcct gagctcctca
      481 gggtccatgg accatcccat ggcatcgtca agggatatga gtttgcgcgt gaaaagagcg
      541 aacagggcct ggttcatcgt ttgcatgccg tgtttgccct gaccgacctg catctgggaa
      601 tagatctggt ggatcttgtc ttcccggatc aggtttctga tggcgggcgt cgagatcatc
      661 agctccgcgg ccagaactct gcccttgccg tccgccctcg gcagcagctg ctgcgagatc
      721 accgcctcca gaacgaagga catctgggcc cggatctggg actgctgatg cgcagggaat
      781 gcatccacga ctcggttgac agtctgaacc gcggaattgg tgtggagagt cgcgaatacc
      841 agatgccctg tttccgcaat ggtgagcgca gcctgcatgg tttcaaggtc gcgcatctcg
      901 ccgatgaaca cgacatcagg gtcctgcctg aggatatatt tcagcgcatt ggtaaagttc
      961 ttggtatcgg agccaacctc tctctggttc acgatgcacc ctttgtgctt atgtacgaat
     1021 tcaatgggat cctcgattgt aacgatgtgg tcgcgccgtt ctgtattgat cttgtcgagg
     1081 aacgacgcaa gcgttgtgga tttcccgctc cccgtaggcc cggtgacgag cacgagccct
     1141 ctgggcagtg tggtcagcct gcctacgaca ttgggaagcc cgagggtctc aagggggatg
     1201 atgtcgaagg gaat
//
LOCUS       DAOQUC010001443         1214 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_165819_length_1214_cov_2.204487, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001443 DAOQUC010000000
VERSION     DAOQUC010001443.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1214
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_165819_length_1214_cov_2.204487"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..838
                     /gene="flgA"
                     /locus_tag="PKH07_18970"
     CDS             <1..838
                     /gene="flgA"
                     /locus_tag="PKH07_18970"
                     /inference="COORDINATES: protein motif:HMM:TIGR03170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HNT37080.1"
                     /translation="GDIADVSGSQAALLSKVLLQSAPLTGRTQSIKAPYVLLKLRQSG
                     FDTDSLELTGAQAVKMSTPGNLVPSHELAQRFEDGLRERLGADADRCEISLNRAPTPL
                     FVPDGTYEWEVRPSSANLLGTVPVQLLAKNPESGQVVASTRGCAQVRRFEEVLVSTRE
                     IARGSTITDKDLNTERKEITNLLQLRDLITDPKAAVGLKARRFIALGETLKTSSLERE
                     KVVAYGQTVLIEIEGTGFVITAPGVARQAGSMGDQIRVKSTTGGQEMLTQVIGPGKVR
                     AF"
     gene            895..>1214
                     /locus_tag="PKH07_18975"
     CDS             895..>1214
                     /locus_tag="PKH07_18975"
                     /inference="COORDINATES: protein motif:HMM:NF014195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HNT37081.1"
                     /translation="MATKMLFVFLLTLQTAAPSNNTRMRTIDLYSDNRAYRVNDIVTV
                     LITESSSASEEAKTVTGKKNSLQAKVSALWNNNIAGKIFGKAGDSVDYPSMDITSDMS
                     FDGNG"
BASE COUNT          292 a          331 c          345 g          246 t
ORIGIN      
        1 cggagatatc gccgatgtgt cgggaagtca ggcggctctg ttgtcaaagg ttctgcttca
       61 gagcgcccca ttgaccggac gcactcagtc catcaaggca ccctacgtcc tcctcaaact
      121 gagacagtcc ggtttcgaca cggattccct ggagctgacc ggcgctcagg cagtcaagat
      181 gagcacaccg ggaaacctgg tgccatcgca cgaattggca caaaggttcg aggacggctt
      241 gcgggaacgc cttggagcgg atgcagatcg ctgtgagatc agcctcaacc gagctcccac
      301 acctcttttt gttcctgatg ggacttatga atgggaggtg cgaccctctt ctgcaaacct
      361 cttgggaacg gtgcccgtcc aactgctggc aaagaatccg gaatcaggcc aagtagttgc
      421 ttccacgcga ggctgtgccc aggttcgtcg gtttgaggaa gttctcgtat ccactcgcga
      481 aatcgctcga ggatcaacga tcacggacaa agatctgaac accgaacgga aggagatcac
      541 caatctccta cagcttcgcg atctcattac ggatccgaag gctgcggttg gactgaaggc
      601 aagacggttc atcgctttgg gcgagacgct caagacctct tccctggaac gggagaaggt
      661 tgttgcgtac ggtcagactg tacttattga gatcgaagga acgggatttg tcatcacggc
      721 tcccggcgtt gcccgccagg cagggagtat gggagaccaa atccgggtca agagcaccac
      781 aggcggacag gagatgctga cacaggtgat tggccccggt aaggtgcgcg ctttttgagg
      841 agattgcgcc agagggctgt ttcgttcagc ccattcaaat cgaaaggatt tctgatggcc
      901 accaaaatgc tctttgtgtt tctgctgacg ctgcagacgg cggcaccctc caacaacaca
      961 cgcatgagaa ccattgatct ctatagcgac aaccgagcct accgcgtcaa tgacatcgtg
     1021 acggtgctga tcaccgaatc ctccagcgcc tctgaagagg ccaagacagt cacgggcaag
     1081 aagaactcac ttcaggcaaa agtgtcggct ctctggaaca acaacattgc tggcaagata
     1141 ttcgggaaag cgggagattc ggtggactat ccctcgatgg acatcacctc ggacatgagc
     1201 ttcgacggca acgg
//
LOCUS       DAOQUC010001444         1214 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_165838_length_1214_cov_2.077653, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001444 DAOQUC010000000
VERSION     DAOQUC010001444.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1214
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_165838_length_1214_cov_2.077653"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..487
                     /locus_tag="PKH07_18980"
     CDS             <1..487
                     /locus_tag="PKH07_18980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004074674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNT37082.1"
                     /translation="NVHSEVGKGTTVKVYLPRCEEEAVPMKREDVEGEILGGTETILL
                     VEDEPAILNLAQMMIERWGYRVLAASTPTEAMQLAQEHAGGIHLLITDVIMPEMNGRD
                     LARSLHSSQPEIRTLFMSGYNADIIAHHGVLDRGVYFIQKPFMMDELAAKIREALAPD
                     I"
     gene            573..>1214
                     /locus_tag="PKH07_18985"
     CDS             573..>1214
                     /locus_tag="PKH07_18985"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HNT37083.1"
                     /translation="MSQVVAVIMAGGKGERFWPFSRRDQPKQMLPLTTDKPLLVETVN
                     RVLPITDFDHIFIVSNERLAPNVLQSLPDLPPQNSLAEPFGRDTAPCLAFSAHVIQQR
                     FGADTIMLVLSADHHVEHQERFRSYLRQAICVAEADLLCTIGLEPDRPEIGYGYLELG
                     EPVAQLGSDVYRVVRFTEKPDEPTAQKMLDSGNYLWNGGMFCWKTGVFLNAMKR"
BASE COUNT          290 a          326 c          327 g          271 t
ORIGIN      
        1 caacgttcac agcgaagtgg gcaaaggaac gaccgtcaag gtctaccttc cccgatgtga
       61 agaagaagcc gtcccaatga agagagagga tgtggagggg gagatcctcg gtggcaccga
      121 aaccatactg cttgtggagg atgaaccggc gatcctgaat ctcgcgcaga tgatgatcga
      181 acgctggggt tacagggtgc tggccgcttc cacgccaaca gaggcgatgc aacttgccca
      241 ggagcacgct ggggggatcc atcttctgat caccgatgtg atcatgcccg aaatgaacgg
      301 acgcgatctg gcaaggagcc ttcattcatc gcaaccggaa atcagaacct tgtttatgtc
      361 tggatacaat gcggatatca tcgcccatca cggtgtgctg gatagagggg tctatttcat
      421 ccagaagcca ttcatgatgg acgaactggc agccaaaatc cgtgaggctc tcgctccgga
      481 catctgagca gactccgcaa agaaggctgg tcgtcttgac ttttcgtggt gttctcctac
      541 cattcctcta tatgttccag gaggtttgat gcgtgagtca agtcgttgcc gtcatcatgg
      601 ctggaggcaa gggagagaga ttctggccat tcagccgtcg ggatcaaccc aagcagatgc
      661 tccctttgac aacggacaaa ccgcttctgg ttgaaacagt caacagagtg ctccccatca
      721 cggacttcga tcacattttt atcgtctcca acgagaggct tgcccccaat gttctgcaat
      781 ctctgcccga cctgccaccg cagaacagcc ttgcagaacc cttcggtcgt gatacggcac
      841 cctgtctggc tttttcagcg cacgtgatcc agcagcgatt cggtgccgat accatcatgc
      901 tggtactctc ggcagaccat cacgtagagc atcaggagcg attccgaagc tatctccgcc
      961 aagccatctg tgtcgccgaa gcagatctcc tgtgcacgat cggtctggag ccagaccgtc
     1021 ctgagatcgg atatgggtac cttgaactcg gagagcctgt ggctcaactt ggatccgatg
     1081 tctatcgtgt tgttcgcttt accgaaaagc cagatgaacc aaccgcacag aaaatgcttg
     1141 acagtggcaa ttacctctgg aatggaggca tgttctgttg gaagacagga gtctttctca
     1201 atgccatgaa gcga
//
LOCUS       DAOQUC010001445         1213 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_165960_length_1213_cov_3.014680, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001445 DAOQUC010000000
VERSION     DAOQUC010001445.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1213
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_165960_length_1213_cov_3.014680"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(163..849)
                     /locus_tag="PKH07_18990"
     CDS             complement(163..849)
                     /locus_tag="PKH07_18990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008100199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37084.1"
                     /translation="MKRGFWTKSALMVLMLAVICPVISAQDESDGKAPLEIKLPKPMF
                     VGTPKNIQSTNLEKPTGAKRPPFMAPQGTKNVALGKAVSGSDEDPVIGDMQQITDGDK
                     EAVDGSYVEFGPGLQHIQVDLGAEHLLYAIAFWHYHQQARVYKDVVVQIADDPDFIMN
                     VRTLFSNDHDNSSGLGVGKDKEYIETYEGKLIDAKGEKARYVRLYSDGNTANDMNHYI
                     EIEVYGKPAK"
     gene            complement(939..>1213)
                     /locus_tag="PKH07_18995"
     CDS             complement(939..>1213)
                     /locus_tag="PKH07_18995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37085.1"
                     /translation="DKVDHVKTVPLDEAIRKGTVAVSIIFTDNGQKANVTLTRQEGHG
                     GFHVRITQGLHNLPSGGRTVQVRADSELTLNLATSSIATASLAVAR"
BASE COUNT          267 a          341 c          291 g          314 t
ORIGIN      
        1 tatgcattgt cacaccaaac aatgtttttg gactgaccga tatttcagaa cgcttccccc
       61 ttcaaaagga gaacggccga ttggaaggct gaaaacacat tccgatggcc gttcagaaat
      121 agacctatcc agtcatccaa acagtatgac ccgtggttga gactacttgg ccggctttcc
      181 gtagacttcg atctcgatgt agtgattcat atcgtttgcc gtgttgccat cgctgtagag
      241 acgaacgtag cgcgccttct ctcctttggc gtcaatgagt ttgccctcgt aggtctcaat
      301 gtattccttg tccttgccca cgcccaaccc agacgagttg tcgtggtcgt tgctgaacag
      361 cgttcgaacg ttcatgatga aatccggatc atcggcaatc tgcaccacga cgtctttgta
      421 gactcttgcc tgctggtggt aatgccagaa ggcgatggcg tacaacagat gctcggcacc
      481 caaatctacc tgaatatgct ggagtcctgg gccgaactca acgtaagaac cgtcaacggc
      541 ttccttgtca ccatcggtaa tctgctgcat gtctccgata accggatcct cgtcactccc
      601 gctgaccgct ttgcccaagg caacattctt ggttccctga ggagccataa aaggaggcct
      661 tttggctccc gtgggttttt caaggttggt ggactgaatg ttcttaggag ttccaacaaa
      721 catcggcttg ggcaacttga tctccagagg ggctttgccg tcgctttcat cctgcgccga
      781 gatgacaggg cagatgaccg cgagcatcag aaccatcagt gcactctttg tccaaaatcc
      841 tctcttcatt tcatatcctc cttcaccaat ctgaatctgt aaccgagtca gcgatcagga
      901 tctcatcatc gtcccgatcc actgatcatt gcttctctct atcgagccac agccaaggag
      961 gccgtcgcta ttgatgaggt tgccaggttg agcgtaagct cagagtccgc gcgaacctga
     1021 acggttctgc ccccagaggg caaattgtgc aacccctgcg tgatccgaac atgaaagcca
     1081 ccatggcctt cctgtcgtgt cagtgtgaca ttggccttct gcccgttgtc cgtgaagatg
     1141 atggacacag cgacagtccc ctttcgaatc gcttcgtcaa ggggcactgt cttgacgtga
     1201 tccactttgt ctg
//
LOCUS       DAOQUC010001446         1211 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_166437_length_1211_cov_3.296713, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001446 DAOQUC010000000
VERSION     DAOQUC010001446.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1211
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_166437_length_1211_cov_3.296713"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..281
                     /locus_tag="PKH07_19000"
     CDS             <1..281
                     /locus_tag="PKH07_19000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37086.1"
                     /translation="FAGGRRISPETNQYHYVNWPYAIEKVGWPVFSDLHREYGAYRLH
                     SRGPNLIGPETGIPYDPTNGTNSDGDIIYSPKTGFAVKDISYLDPKNQ"
     gene            complement(293..>1211)
                     /locus_tag="PKH07_19005"
     CDS             complement(293..>1211)
                     /locus_tag="PKH07_19005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37087.1"
                     /translation="KHAATEVPAAVTLEECWELVETAEKQHRHCVMMENCCYDRTEML
                     ILNMVRQGVLGELIHAECGYLHDLRRHKLTNVYEGQWRLKHSIERNGDLYPTHGIGPV
                     AQCMNINRGNQFETLVSMSSKSRGLQLYASDQFGADSPLARQEYALGDVVITLIRTVN
                     GETIVITHDTDSPRPYSRNILVQGTKGIIRKYPEELIHIQGRSAGHDWEPLDAYREQY
                     DHPIWKALADKSRGFGHGGMDYIEDYRLVQSLREGTPTDMDVYDAAAWSAISALSERS
                     IARRSRSLDFPDFTRGAWKDREPLGIISI"
BASE COUNT          264 a          334 c          319 g          294 t
ORIGIN      
        1 ccttcgcggg tggcagaaga atctctcctg agacaaacca gtaccactat gtcaactggc
       61 cttacgctat cgagaaagtt ggatggcctg tgttctcaga tcttcacagg gagtatggtg
      121 cgtaccgctt gcacagccgt ggccccaatc tcatcggacc cgagacaggc attccttacg
      181 atccaaccaa tggaaccaac agcgatgggg acatcatcta ctcgcccaag acgggctttg
      241 ctgtcaagga catttcatat ctggatccaa agaaccagta gactgcgttc catcagatac
      301 tgatgatacc gagaggctct ctgtccttcc atgcgcctcg cgtgaagtca gggaaatcca
      361 gagaccggct tcgtcgggct atggaacgct cactcagcgc ggagatggcg ctccacgcgg
      421 cggcatcgta gacatccatg tcagtgggag ttccttctcg aagagactga accaagcggt
      481 agtcttcgat gtagtccatt cccccgtggc cgaatcctct ggacttgtct gcaagggctt
      541 tccagatcgg atgatcgtac tgctcccgat acgcgtcgag aggctcccaa tcatggcccg
      601 cactgcgtcc ctgaatatga atcagttcct cagggtactt ccgaatgatg cccttggtgc
      661 cttggacgag aatgttgcgg ctgtagggtc gcggtgaatc ggtatcgtgg gtaatgacga
      721 tcgtctcccc gttgactgtt cggatgagcg tgatcacgac atcacccaga gcgtactcct
      781 gtctcgccag cggactatca gctccaaatt gatccgaggc atacaactgg agtcctcttg
      841 acttggaact catggagacg agcgtttcga attgattacc tcggttgatg ttcatacatt
      901 gtgcgactgg cccgatgccg tgtgtgggat acagatcgcc gttgcgttcg attgaatgct
      961 tcagtcgcca ttgcccctca tagacattcg tgagtttgtg ccgacgcaga tcatgaaggt
     1021 aaccgcattc cgcatggatg agctcaccaa gaacaccttg gcgaaccatg ttgaggataa
     1081 gcatctccgt tcggtcgtag cagcagtttt ccatcatcac gcagtgtcta tgctgcttct
     1141 cggcggtttc aaccaattcc cagcattcct cgagtgtcac cgcagcagga acttctgtgg
     1201 cagcgtgttt g
//
LOCUS       DAOQUC010001447         1211 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_166610_length_1211_cov_1.811419, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001447 DAOQUC010000000
VERSION     DAOQUC010001447.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1211
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_166610_length_1211_cov_1.811419"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..458)
                     /locus_tag="PKH07_19010"
     CDS             complement(<1..458)
                     /locus_tag="PKH07_19010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HNT37088.1"
                     /translation="MRAFVFVVLAFGTLFLSAKVSLAASQASATEAAESLGWRLGMQA
                     YSFNRFTFFEAVEKTAALGMKWIEAYPGQKMSQEKPDSKVHHTMSQEDMDAVLAKLKA
                     EGVQLVNYGVVGLGNDEAECRKVFEFAKKMGIETITSEPEEGALDLIDRL"
     gene            complement(516..>1211)
                     /locus_tag="PKH07_19015"
     CDS             complement(516..>1211)
                     /locus_tag="PKH07_19015"
                     /inference="COORDINATES: protein motif:HMM:NF039847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNT37089.1"
                     /translation="GDYTGIQPPMPIPEGFNYDFWLGPAPWAPYTAGRCHFNFRWLMD
                     YSEGYISDWGAHYYDVAQWGNGSDLTSPVLVQGTGVFPQDGLYDAAVEHHIEYTYANG
                     VKMISSTDLPGAVRFEGTEGWVHVDAGPTGLETFPESLKQTTIAPNEIHLYDSTDHHR
                     NFIDCVKTRRETAAPVEVGHRSAALCHLGSIAVVVGRPLKWDPEKECFPEDAEAQRLL
                     ARSMRSPWNLQAL"
BASE COUNT          282 a          347 c          303 g          279 t
ORIGIN      
        1 cacaagcggt caatgagatc cagtgcgccc tcttcaggtt ccgacgtgat cgtctcgata
       61 cccatcttct tcgcaaactc aaagaccttg cggcactccg cctcgtcgtt tccaagtcct
      121 accacgccgt agttgaccag ctgcactcct tcggctttga gcttagccaa aacggcatcc
      181 atatcttctt gagacatcgt gtggtgaacc tttgagtcgg gcttctcctg actcatcttc
      241 tgccctggat aggcttcgat ccacttcatg ccaagcgctg ccgtcttttc aactgcttca
      301 aaaaacgtga agcggttgaa tgaatatgcc tgcattccca gtcgccatcc aagcgactca
      361 gccgcttccg ttgccgatgc ttgggacgcc gcaagggaga ctttggcgga aagaaacaaa
      421 gtgccgaatg ccaatacgac aaacacaaat gctctcatca ggatgacctc tttcagacta
      481 tcatgttcag tccattgcgg gactatttcc caaacttaca gggcttgaag attccaaggg
      541 ctgcgcatgc tccgggcgag gagcctctga gcttccgcgt cttcagggaa acattccttc
      601 tcgggatccc acttcagagg gcggccgacg acgacggcga tacttcccag atgacacaga
      661 gccgcagaac ggtgtccgac ctcaacgggc gcagctgtct cgcgtctcgt cttcacacag
      721 tcaatgaagt tccgatggtg atcggtgcta tcgtaaaggt gaatttcgtt gggagcaatc
      781 gtagtctgct tcagggactc ggggaaagtc tcaagtccgg ttggtcctgc gtcaacgtga
      841 acccaacctt cagtaccttc aaaacggacg gcacctggaa gatcagtgga agagatcatc
      901 ttcacgccgt tggcataggt atattcaatg tgatgctcca ccgctgcgtc atacaaaccg
      961 tcttggggaa agactcctgt gccctgaacc agcacaggcg aagtcaaatc agatccattt
     1021 ccccattgcg ccacgtcgta atagtgcgcg ccccagtcgg agatgtaacc ctctgaatag
     1081 tccatcagcc atcggaaatt gaaatggcag cgtcctgctg tatagggtgc ccacggagcg
     1141 ggaccgagcc agaaatcgta attgaaacct tctggaatcg gcatcggagg ttggatgccc
     1201 gtgtaatcgc c
//
LOCUS       DAOQUC010001448         1210 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_166710_length_1210_cov_2.986147, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001448 DAOQUC010000000
VERSION     DAOQUC010001448.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1210
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_166710_length_1210_cov_2.986147"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..511)
                     /locus_tag="PKH07_19020"
     CDS             complement(<1..511)
                     /locus_tag="PKH07_19020"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNT37090.1"
                     /translation="MVLNYQPESRDFERPDMALVPEGEFLFGGAYGLFHERFSLFAKA
                     ILIDMNFHEDMVVAPEWICLEQFAIGTCEVTNGEYLAFVDSGDYRVPAYWEPFAYEVK
                     MKQGFPPPISLDVSAIERQERLWSVRPVYGASYDDCLAYCSWLSAKTGVVYRLPTEQE
                     WEKAVCWNPT"
     gene            complement(648..>1210)
                     /locus_tag="PKH07_19025"
     CDS             complement(648..>1210)
                     /locus_tag="PKH07_19025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37091.1"
                     /translation="DSDVALYYFGGKRSWFSPERGMYLQHGSAYTLPVPPILFDPNVT
                     TPLASCPLLEKSVDGSSNFAGIEPVLRDVYVYPVPNFNEDPYQCAMRIYNKAEKTRGH
                     NDHWRHCVVSCQISRECILGFILSGACGIADEIRDAFRELDPGEFSLGDLWADAYGAL
                     AASMSGFSCEDACESEFPGKKRDCYF"
BASE COUNT          316 a          330 c          288 g          276 t
ORIGIN      
        1 atgttggatt ccagcatacg gccttctccc attcctgttc agtgggaagt cggtacacaa
       61 ctcctgtctt tgcactcaac catgagcagt acgcaaggca atcatcataa cttgcaccat
      121 aaactggccg aactgaccac aagcgctcct gtctctcaat cgcgcttacg tccagagaaa
      181 tgggtggtgg gaaaccttgc ttcatcttca cttcataggc gaaaggttcc cagtaggcag
      241 gaacccggta atcacctgaa tccacaaaag ccaggtactc cccattcgtg acctcgcatg
      301 ttccgatggc aaactgctcc aggcaaatcc attcaggggc aaccaccata tcctcatgaa
      361 agttcatatc aatgagaatc gccttcgcga aaagtgagaa tctctcgtgg aaaagaccat
      421 aggcgccgcc aaagaggaat tcaccctctg gaacaagagc catatctggg cgttcgaaat
      481 cccgagattc cggttggtag ttgagaacca tgagcctgag ccacctttgg ctagacacat
      541 tgaccaactt caggacgaca aacatcagga gcgcaatgca gcagaccaaa aggaactttg
      601 gcaccttctt ggttttgtga atgtgtacct tgcctggatg tgaattgtta gaagtaacag
      661 tcacgcttct tccccggaaa ctcactttcg catgcatctt cacagctgaa accggacatt
      721 gaggccgcga gtgcgccgta tgcatccgcc catagatctc cgagactgaa ttcccctgga
      781 tccagctccc taaaagcatc acggatctca tctgcaatac cacacgcccc ggaaaggatg
      841 aaccccagta tacactctct cgagatctga caactcacga cgcagtgtct ccaatggtca
      901 ttatggcccc tagtcttctc agccttgttg tagattctca tcgcgcattg atatggatct
      961 tcattgaagt ttggcacagg atagacgtag acgtctcgaa ggacaggctc tattcccgcg
     1021 aagttgctag atccgtctac agacttctcc aacaacgggc aagaagctag tggagtagtg
     1081 acgttggggt cgaagagaat tggcggtacc ggcagtgtgt atgcgctccc atgctgcaaa
     1141 tacatgccgc gttctgggga gaaccaggag cgtttgccgc cgaagtagta cagggcaacg
     1201 tcggagtcgt
//
LOCUS       DAOQUC010001449         1210 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_166761_length_1210_cov_2.432900, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001449 DAOQUC010000000
VERSION     DAOQUC010001449.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1210
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_166761_length_1210_cov_2.432900"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..634)
                     /locus_tag="PKH07_19030"
     CDS             complement(<1..634)
                     /locus_tag="PKH07_19030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011937090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HNT37092.1"
                     /translation="MSHSENLYKPMLMEIQKVIPETPDVRTFELVFKDSADRDAFRFR
                     AGQFAEYSAFGLGESTFCIASSPTRMDHIECCFRKTGRVTSELFRLNEGDTIGLRGPY
                     GNSFPIEDCFGKNLYFVAGGIALPPLRTLIWNCLDWRDKFGEITIVYGARTVSDLVYK
                     RELDEWQKRSDIKLVKTVDPGGQTPDWDGSVGFVPTVLKEVGPSPSNSVAF"
     gene            complement(647..>1210)
                     /locus_tag="PKH07_19035"
     CDS             complement(647..>1210)
                     /locus_tag="PKH07_19035"
                     /inference="COORDINATES: protein motif:HMM:NF028488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNT37093.1"
                     /translation="DVFMLRQSDGSYLVEAVTEKGEALLKQTSKSLELSEAQPNADAE
                     DIVPRRFDLNRVKPWLDEHFDDDFWKKMSLKCIGCGTCTFLCPTCHCFDLVDETRQGA
                     GVRRKNWDSCQFSLFTLHASGHNPRKTQSERYRQRIMHKFKYYQEKFGKTLCVGCGRC
                     VRSCPVGHSLLEYLTEIDRLSREAQPK"
BASE COUNT          295 a          338 c          293 g          284 t
ORIGIN      
        1 caaaagccac tgaattgctg ggactgggac ccacttcttt gagaaccgtt ggcacaaagc
       61 cgacggaacc atcccagtca ggcgtctgtc caccgggatc cacggtcttc accaacttaa
      121 tgtcggaacg cttctgccac tcgtcaagtt cacgtttgta gacgagatcg ctgacagttc
      181 gagcaccata gacgatagtg atctctccaa acttgtcgcg ccagtccagg cagttccaga
      241 tgagcgttct caaaggcggc aatgcaatac caccggccac aaagtagagg ttcttgccaa
      301 agcagtcttc aataggaaat gaattcccgt agggaccgcg aaggcctatg gtgtctcctt
      361 cgttgagacg aaacagttcc gaagtcacac gtcccgtctt gcgaaaacag cattcgatgt
      421 gatccatgcg tgtgggcgaa ctggcaatgc aaaacgtgct ctcgcccagt ccaaacgcgg
      481 aatactctgc aaactgaccg gcacgaaaac gaaaggcatc gcgatctgca gagtctttga
      541 agaccagttc gaacgtgcgc acgtcgggag tctcagggat cactttctgg atttccatga
      601 gcattggttt gtagagattc tctgagtgcg acatcggatt ctccatctat ttcggctgcg
      661 cttcgcgtga gagtctgtct atttctgtga ggtattccaa cagactgtgt cccaccggac
      721 aggagcggac acatcgccca catcccacgc aaagagtctt gccaaacttc tcttgatagt
      781 atttgaactt gtgcatgata cgctgccgat atctctcgct ctgagtctta cggggattgt
      841 gtcccgaagc gtgcagcgtg aagagactga actggcaact gtcccagttc ttgcgccgaa
      901 cccctgcacc ctgccttgtc tcatccacca gatcaaagca gtgacacgtc ggacagagga
      961 aagtgcaggt tccgcatcca atgcacttga gtgacatctt cttccagaaa tcgtcatcaa
     1021 aatgctcatc caaccacggt ttgacgcgat tgagatcgaa tcgtctcgga acgatatcct
     1081 cggcatctgc gttcggctgc gcctcagaca gctcaagtga ctttgatgtc tgcttcagaa
     1141 gggcttcccc cttctccgtg actgcctcaa caagataact gccatcagat tgtctcagca
     1201 tgaagacatc
//
LOCUS       DAOQUC010001450         1208 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_167164_length_1208_cov_3.665221, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001450 DAOQUC010000000
VERSION     DAOQUC010001450.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1208
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_167164_length_1208_cov_3.665221"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            257..703
                     /gene="aroQ"
                     /locus_tag="PKH07_19040"
     CDS             257..703
                     /gene="aroQ"
                     /locus_tag="PKH07_19040"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HNT37094.1"
                     /translation="MRILVIHGPNLNLLGCREPAIYGRETLEDIDTSLQALAEELGVQ
                     LRTVQLSSEGAIIDYLIENAQWADGLLINPAAYTHTSVAIADTIKGLELPTIEVHLSN
                     VHAREEFRRHSYIAPVSIGQICGMGSNSYLLGLRGLAAWLTKTKLI"
     gene            896..>1208
                     /locus_tag="PKH07_19045"
     CDS             896..>1208
                     /locus_tag="PKH07_19045"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNT37095.1"
                     /translation="MKAVILAAGKGTRMKYLTADKPKPMVDVGKELILERILGTIRDA
                     GICDFIVVTGYFGNIIEEHFKDGRDFGMNIRYVRQEVQDGTGSALHLTRDAVGDEPFY
                     MS"
BASE COUNT          283 a          290 c          335 g          300 t
ORIGIN      
        1 ctatcatcac caaagcataa ggaagttcta cctgaaacac tggcacaggt ctttcctctg
       61 tgatattctg gttacgttga gcatctggat tcgctgtgcg gttcttgtca tcatcgcggt
      121 gattggtggc ttctttcgag caggttagca cctgcgggcg tgtgggctat gcccactccc
      181 tcgaacgctc gctcggtaga tgatacgcct gttccgaact gagtgtcact tgaaaaggag
      241 gttaggagga aaaagcatga gaatccttgt cattcacggt ccgaatctga atcttttggg
      301 ctgtcgggaa ccggccattt atggcaggga gacgcttgag gatattgaca ccagtcttca
      361 ggcgctcgcc gaagaactcg gtgtgcaact gcgcactgtc cagctgtctt ccgagggagc
      421 gatcattgac tatctgattg aaaacgcgca gtgggcagat gggctgttga tcaatccggc
      481 agcatacacg cacacatctg tcgccatcgc ggatacgatc aagggtttgg agttgcctac
      541 catcgaagtg catctctcca acgttcacgc gcgtgaagag ttccgaaggc attcgtatat
      601 tgcccctgtt tccattggac agatatgcgg aatgggttct aacagctacc ttctcggcct
      661 gcgtggactg gcagcctggc tgacaaagac aaaattgatc taatctggag cctctatgcg
      721 tttctgagcg cgtatgctga tcgtgcctac aagatcaagc ggctgattca ttcgtgggtg
      781 gtgcatacct tacggttgag aatgagtccc atctcgtaaa ccggaatccg tttgacaaac
      841 aggtcatctt ttgtacgatt ccgagaaacg tcttttcgta tggagaaagg ttggcatgaa
      901 ggcagttatc ttggccgcgg gcaaaggcac ccgcatgaag tatctgacgg cggacaagcc
      961 gaaacctatg gtagatgttg gcaaagaact cattctcgag aggattctcg gaacaatacg
     1021 tgacgcgggg atttgtgatt tcattgtggt gacaggctac ttcggcaaca tcattgaaga
     1081 acatttcaag gatggacgag acttcggcat gaacatccgg tacgtccgcc aggaagtgca
     1141 ggacggcacc ggcagcgccc ttcatctgac acgagacgcg gtgggagatg agccgttcta
     1201 catgtcct
//
LOCUS       DAOQUC010001451         1208 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_167206_length_1208_cov_2.876843, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001451 DAOQUC010000000
VERSION     DAOQUC010001451.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1208
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_167206_length_1208_cov_2.876843"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            39..>1208
                     /locus_tag="PKH07_19050"
     CDS             39..>1208
                     /locus_tag="PKH07_19050"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNT37096.1"
                     /translation="MNGVGAARSLGYTGQGVKIAIIDSGVDFAHPDLFETQARVEDAN
                     SPYYGWPICFDGRSMAAYSYYGSTEDTWYADTSTIPDVTFEGDTGTAQFATVIDGAPV
                     VKTYTFNKESVSGKYHFGLHPDTYLRDEVWGGRTAAILVVDHPDVENRGAGYNTVYVD
                     LNNNYDFRDDKPCYRGNEVSYLDFWDATAWDYGTDGFPDISGGMVYFIGDGVNPIPAS
                     DWLFDGDIPIPGNGDLVAFMLNNPDESGGNHGTFCASAAVGRGIINGDAPAIKPTYAG
                     AGDGMVQGPAPNAKIIAVGNYYQGGFEADFYLFSAFGYDGVAGTGDEPNIVSMSYGDG
                     AIDNDGWDYWSRYLDYLAKNVAPEVTFVISSGNGAPGFATVNSPGPTSAIKVGAST"
BASE COUNT          275 a          309 c          347 g          277 t
ORIGIN      
        1 caaaacttct acaaagcgac agtccatggc ttgatcagat gaatggagtc ggtgctgctc
       61 gttcactcgg ttacacgggc caaggcgtca agatcgccat catagattcg ggcgttgatt
      121 ttgcacatcc ggacttgttc gaaacgcaag cccgagttga agacgcaaat tcaccgtact
      181 atggatggcc aatctgtttc gatgggcgtt cgatggctgc ttattcgtat tatggcagca
      241 ccgaagatac gtggtatgcg gacaccagca cgattccgga tgtcactttc gaaggagaca
      301 ccggaaccgc acaattcgca acggtcatag atggcgctcc ggtcgtgaag acctacactt
      361 tcaacaagga gagtgtctct ggcaagtatc atttcggact tcacccagat acctatctgc
      421 gggatgaggt ctggggaggc cgcactgccg ccattctggt ggtggatcat ccggatgtgg
      481 aaaacagggg agcaggctac aacacagtct acgtggactt gaacaacaac tatgatttca
      541 gggacgacaa gccctgctat cgtggcaacg aggtctcata tctggacttc tgggatgcca
      601 cagcatggga ctacggcacg gatggatttc ccgacatctc gggcggcatg gtctacttca
      661 tcggcgatgg agtcaatccg attcccgctt ccgattggct ttttgacggt gatataccga
      721 ttccggggaa cggcgatctg gtcgccttca tgctgaacaa tcccgatgag agcgggggca
      781 atcatggaac gttctgcgcc tcggcggctg tggggagagg catcatcaat ggagatgcac
      841 ccgcgatcaa accgacctat gccggagcgg gtgatggcat ggttcaaggg cctgctccta
      901 acgccaagat catcgctgtg ggcaattact atcagggtgg tttcgaggcc gatttctact
      961 tgttctccgc gttcggctac gatggagttg ctggcaccgg tgatgagccg aatatcgtca
     1021 gcatgagcta tggcgatgga gcaatagaca atgacggctg ggactattgg tcgcggtatt
     1081 tggactatct cgccaagaat gttgctccgg aagtcacctt cgtcatctct tcgggcaatg
     1141 gagccccagg ttttgcgaca gtcaactctc ctggaccgac gagcgcaatc aaggtcggcg
     1201 catccacc
//
LOCUS       DAOQUC010001452         1208 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_167211_length_1208_cov_2.792715, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001452 DAOQUC010000000
VERSION     DAOQUC010001452.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1208
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_167211_length_1208_cov_2.792715"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..367)
                     /locus_tag="PKH07_19055"
     CDS             complement(<1..367)
                     /locus_tag="PKH07_19055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003235420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HNT37097.1"
                     /translation="MTITRINNNIAAINATSNLNRTTFDLEKSLERLSSGLRINRASD
                     DASGLTISEKLRSQIRGLNQALKNAEDAVSLVNTAEGALDETTSILQRIRELAVKAAN
                     TGGLDINAIQAAQDEIDSAI"
     gene            complement(784..>1208)
                     /locus_tag="PKH07_19060"
     CDS             complement(784..>1208)
                     /locus_tag="PKH07_19060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37098.1"
                     /translation="CTAHALVSGTAMGAVKGGFRAVGGAFEMALAPLPPYGPVMEPPY
                     PRPLVFNPPCTPAPAMDKVAACGMAAGCQQACPMQMSRCPKAGKCKMNCGAAKCRGWV
                     EGRRGACQKACPKSEREPMKKQQKDQKPQGAGCCPMSK"
BASE COUNT          260 a          338 c          310 g          300 t
ORIGIN      
        1 gaatggccga gtcaatttcg tcctgggctg cctggatagc gttgatatcc aaacctccgg
       61 tgttggctgc cttgaccgcc aattcacgga ttctctgaag aatggatgtg gtctcatcca
      121 acgcgccttc tgccgtattc accagggaca cggcgtcctc cgcgttcttg agagcctgat
      181 tgagaccgcg aatctgactg cgcaacttct cggaaatcgt cagacccgaa gcgtcatcag
      241 aagcgcggtt gattcgcaac cctgaggaca accgttccag ggatttctcc aggtcgaacg
      301 ttgtccgatt tagattactc gtcgcgttaa tcgcggcgat gttgttgttg attcgagtaa
      361 tcgtcacaat tttcctcctt gaagattttg ctcctcagca tccgtgctga ggttccgcca
      421 caaaccccat ccatgcggtt catagtcgga tcttattccg gattcctaca gacgtgcctg
      481 ttcaccttcg aggttcccaa cctctgtgcg aaggatcgct cgggagaaaa cactcccctt
      541 tcccaaatga aggatattta ggcaactcgg aagtaactta gcggaaccaa attggcggaa
      601 ccaagcatca acttatctgc tttgtagcat cggatgattc cgagaaagac ttgagtgatt
      661 ttttctgatg tcctggaaat gaggtggaag tgaagaagaa aagcacccgc agaagagcat
      721 agtccctatg ttgatctgcg ggtgcctatc ctgtctggtc aacttaatcc ggaagcagag
      781 gccttatttg gacatcggac agcagccagc gccctgtggc ttctggtctt tctgctgttt
      841 cttcataggt tcccgttcgc tttttgggca agccttttgg caggctccgc gacgtccctc
      901 gacccacccc ctgcatttgg cagcgccaca gttcatcttg cacttgcctg cctttggaca
      961 ccgggacatc tgcatgggac atgcctgctg acatcctgca gccatgccgc acgccgccac
     1021 cttatccatg gccggtgctg gggtacacgg cggattgaat accagtggtc tggggtacgg
     1081 tggttccatc actggaccat agggaggcaa tggagcaaga gccatctcaa aagctcctcc
     1141 aaccgctcgg aaaccgcctt tgacagcgcc cattgctgtg cccgacacga gagcatgagc
     1201 ggtacaga
//
LOCUS       DAOQUC010001453         1207 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_167433_length_1207_cov_3.360243, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001453 DAOQUC010000000
VERSION     DAOQUC010001453.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1207
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_167433_length_1207_cov_3.360243"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..898)
                     /locus_tag="PKH07_19065"
     CDS             complement(<1..898)
                     /locus_tag="PKH07_19065"
                     /inference="COORDINATES: protein motif:HMM:NF012662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HNT37099.1"
                     /translation="MFENLQTRLSNIFRQMTGKHRLSPENIKEALGEVRLALLEADVN
                     YRTVRSFIRAVEEKAVGQEVLQSLEPGQQVIKIVHSELIRLLGEQEAELQFQSDQRAI
                     IMMVGLQGSGKTTTSAKLSLNLKQEKNRRPGLVAADVYRPAAIQQLQTVGQQVGVPVF
                     TIPGEMDPVRICREALRWADENRVDTLLLDTAGRLHIDQDMMDELVRLKKALQPNYTL
                     LVLDAMTGQDAVRQAATFQETLGIDGGIMTKLDGDSRGGAALSFKSIVDKPIYYAGVG
                     EKITELERFRPDRLASRVLGMGD"
     gene            complement(962..>1207)
                     /locus_tag="PKH07_19070"
     CDS             complement(962..>1207)
                     /locus_tag="PKH07_19070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37100.1"
                     /translation="AVNKGKRSLGKYEKHLHLNENPPSGPAVQQADLAGKSQEIIRSI
                     RFDLEETPLYDNSKVLKQLRDLEELIQTTVNGTGSSS"
BASE COUNT          241 a          349 c          308 g          309 t
ORIGIN      
        1 cgtcacccat tccgagaact ctcgacgcca gacgatcggg acggaatcgc tccaattcgg
       61 tgatcttctc acccacaccg gcatagtaga ttggcttatc tacgatggat ttgaacgaaa
      121 gggcagcgcc accgcgactg tctccgtcca acttggtcat gatcccgccg tcaatgccaa
      181 gtgtttcctg gaacgttgct gcctggcgaa ccgcatcctg accggtcatc gcgtccagaa
      241 ccagcagtgt atagttgggc tgcaacgcct tcttcagccg aaccagttcg tccatcatgt
      301 cctgatcgat gtgcaggcga ccagcggtat ccagcagcaa tgtgtccacg cggttttcat
      361 ctgcccaccg aagcgcctct cgacagattc gaaccggatc catctcccct gggatggtga
      421 agacagggac accgacctgc tgtcccaccg tctgcagttg ttgaatcgct gcggggcgat
      481 agacatctgc cgcaaccaat ccagggcgac ggtttttttc ctgcttcagg ttcagtgaga
      541 gttttgctga agtggttgtc ttgcctgaac cttgaagacc aaccatcatg atgatggcac
      601 gttggtcaga ctgaaactgg agttcggctt cctgttcccc aagcagccga atcaactcgg
      661 aatggacgat cttgatgacc tgttggcctg gctccagact ctgcagaact tcctggccaa
      721 ccgccttctc ctcgacggca cggatgaaac tccttaccgt tcggtagttg acatctgctt
      781 ccagaagcgc caggcggact tctccaagtg cttccttgat attctcaggg ctgagacggt
      841 gcttgcccgt catttgccgg aagatgttgc tcagtctcgt ttgtagattc tcgaacacgt
      901 ggtcttccca aactccggag cagatggccg atggcgatct gctcgagctc tactccattt
      961 cctatgatga actgccggtg ccattcaccg ttgtttgtat gagttcttcc aagtcgcgca
     1021 actgcttgag taccttcgag ttgtcgtaca ggggcgtctc ctcaaggtca aatcttatgc
     1081 ttcggattat ctcctgcgac ttcccagcaa gatcagcttg ctgcacggcc ggacctgaag
     1141 gtggattctc gttgagatgg aggtgtttct catacttccc aagtgagcgt ttgcctttgt
     1201 tgacagc
//
LOCUS       DAOQUC010001454         1206 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_167700_length_1206_cov_2.409209, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001454 DAOQUC010000000
VERSION     DAOQUC010001454.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1206
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_167700_length_1206_cov_2.409209"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            41..>1206
                     /locus_tag="PKH07_19075"
     CDS             41..>1206
                     /locus_tag="PKH07_19075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37101.1"
                     /translation="MAQMATGLAKPVEDGVWIRPAFETDSEPYWAIKNGIGISLWPRT
                     PVRGLIGIHAPYLGFPEGRVVNFVAIEPLVRGRRGYSEMERSGLDDRQGLRLWSANEL
                     NESPEISVPHFPAKGVLSELDGVQTLSFYVYAEPFGNGARPIVQVVLRQDRPHEVTFR
                     TFVTTDSAPMDACILTATMGNYGRLRRLWLKDEIVLSTDLWPQFAPENERFAPHHQWP
                     LEKIRVFDDEAWVAASPNESDLVEVDYVEAVQWWWKYTGTPATHYWRKPQPHSSLMTR
                     VNGRSNYYGGRGSRIPGGIAYENFELQESFTKGAEFSFGVTPETPEALGFVSGIAPSQ
                     EDRSRMSNTKENFVAAYIHLQRCYSASDEFEKREQSIGKQLDLLKETGIRIVMP"
BASE COUNT          298 a          306 c          324 g          278 t
ORIGIN      
        1 tggaaacatg aacatcccaa gcatagtggt tacaattctc atggcacaaa tggcaactgg
       61 tctggcaaaa cctgtcgagg atggcgtctg gattcgtcct gcttttgaga cggattcaga
      121 accttactgg gcaatcaaga acggtattgg catatcactc tggcctcgca ctccggtcag
      181 aggtctgatc ggcatccatg caccctatct gggctttccc gaagggaggg ttgtcaattt
      241 cgttgccatc gaacctttgg tcagaggaag gcgaggttac tccgagatgg aacgaagcgg
      301 actcgacgac agacaaggac tccgcctgtg gtcggccaat gaattgaacg agtctccgga
      361 aatcagcgtg cctcatttcc ccgcaaaggg agttctgagc gaattggatg gggtacaaac
      421 actgtctttc tatgtctacg ctgagccttt cggaaatggt gccagaccga ttgtacaagt
      481 tgttctgcgc caggatcgcc cacatgaggt cacgttcaga acctttgtga caacggacag
      541 cgctcccatg gatgcctgca ttctaacggc tacgatgggc aactacggaa ggcttcgccg
      601 cctctggctc aaggatgaga tcgtactttc aaccgatctc tggcctcagt tcgctcctga
      661 aaacgagaga tttgcacctc atcatcaatg gccgttggag aagattcgcg tcttcgatga
      721 tgaggcctgg gtagccgctt ctccgaacga gtccgatttg gtcgaagtgg actatgttga
      781 agcggttcag tggtggtgga agtatacagg cactccagca acgcattact ggcgcaagcc
      841 acaacctcac tcaagtctga tgacacgtgt gaacggacga tccaattact acggtggacg
      901 tggttccagg atcccaggtg gaattgccta tgagaacttt gagctccagg aatcgttcac
      961 caaaggcgcc gagttcagtt ttggagtgac acccgagact ccggaagcct tgggcttcgt
     1021 ctctggcatt gcaccatcac aagaagatcg ctcaaggatg tcgaacacga aagagaactt
     1081 cgttgccgca tacattcacc tgcagcggtg ttacagtgcc tcggatgaat ttgaaaagcg
     1141 cgagcagtcc atcggcaaac agttagacct tctcaaagaa acgggcatac gaattgtgat
     1201 gcccta
//
LOCUS       DAOQUC010001455         1203 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_168418_length_1203_cov_2.620209, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001455 DAOQUC010000000
VERSION     DAOQUC010001455.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1203
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_168418_length_1203_cov_2.620209"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            59..>1203
                     /locus_tag="PKH07_19080"
     CDS             59..>1203
                     /locus_tag="PKH07_19080"
                     /inference="COORDINATES: protein motif:HMM:NF015377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine peptidase family C39 domain-containing
                     protein"
                     /protein_id="HNT37102.1"
                     /translation="MKERLLLDFDRLILEHPEDSFLASLAKVRKASVVRELAFVGRRT
                     YEQALAEAEAVLSDYPEAHPDVRGLATILIVENLVDLGRYEDALQQGEYFLSCKPEET
                     GQTRQAAATHFFMAKAWIALGCYDRAAELLEALLDRYEPSQVFKTELPRALETVQTVY
                     LDWGTRRSAAGDHAGASRAYTELLLRHTDGEMAARARLELAKEQYRLGEYRTAGLLFR
                     DLAVLSPGTRESDEADEWIAKMFRVVPEANQDAMLDRLTFERYGICGPFALQRILEVF
                     EVRERLQTIVERARTDESGTSIRDMVAACLQSGLRAQVVLVDRAKWALIPVPAIGLSD
                     SGSYFAVLGVKGALGLMDIYDQFGRRTAPAESVMSDWDGLAILVGET"
BASE COUNT          267 a          276 c          397 g          263 t
ORIGIN      
        1 caagacaagg gtcaataata tcaattggcg catcagcgag atggagaagg accccgattt
       61 gaaggaaagg cttctcctgg atttcgaccg gctgattctg gaacacccgg aagatagttt
      121 tctggcatcg ctggcgaagg tgcgaaaagc gtcggtggta cgagagttgg cgtttgtggg
      181 acggcgaacc tacgaacagg cgcttgcaga agccgaagca gtgttgagcg actaccccga
      241 ggcacatcct gacgtcagag gtctggcgac gatcctgatc gttgaaaacc tcgttgattt
      301 gggacggtat gaagacgccc tgcaacaggg tgagtatttc ctttcatgca aacccgagga
      361 gacaggtcag actcgacagg cagcggcaac gcacttcttc atggcaaagg catggatagc
      421 tctcggatgc tacgataggg cagcggaatt actcgaggca ctcttggata ggtatgagcc
      481 gagtcaggtt ttcaagactg agttgcctcg cgctttggaa acggtgcaga cagtgtacct
      541 ggattgggga acgaggcgct cggccgcggg agaccacgcc ggggcatcca gggcatatac
      601 cgaactgctg cttcggcaca cggacggtga gatggctgcg cgagcgagac ttgagttggc
      661 caaagagcag tatcgtttgg gtgagtacag aacggcgggt ttgctgtttc gggatctcgc
      721 tgtactgtca cccgggacac gtgaatctga cgaggcggac gaatggatcg caaagatgtt
      781 ccgggttgtg ccggaggcca atcaggatgc catgctcgac agactgacat tcgaacgcta
      841 tgggatttgc ggtccattcg cactacagcg gatactcgag gtttttgagg ttcgggagcg
      901 acttcagaca atcgtggaac gagcgcggac ggacgagtca gggacatcca ttcgggatat
      961 ggtcgccgcg tgtttgcagt ctggtctgag agcccaggtg gtcttggttg atagggcgaa
     1021 gtgggcgttg attcctgtgc ctgcgatagg tctttcggac tcaggatcgt attttgcagt
     1081 tctcggtgtg aagggtgctt tggggctgat ggacatctac gatcagttcg gcagacgaac
     1141 tgcccccgct gaatcagtca tgtccgactg ggacggcttg gcgattctcg ttggggagac
     1201 aga
//
LOCUS       DAOQUC010001456         1202 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_168619_length_1202_cov_3.482999, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001456 DAOQUC010000000
VERSION     DAOQUC010001456.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1202
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_168619_length_1202_cov_3.482999"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1202)
                     /locus_tag="PKH07_19085"
     CDS             complement(<1..>1202)
                     /locus_tag="PKH07_19085"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNT37103.1"
                     /translation="RLAFFSPLPPVHSGISDYSKELIPHLLPHFDMTLFATQRDVDLK
                     GLESLDIRPIKSFRKSHRERPYDHLLYQMGNGLDHEEIYETALRFPGIVVLHDFILHH
                     FIAGITLDRGRYDAYEAELCDEYGLQQGRELARRMVAPGTSAQDRFRLFMQHPLNRKL
                     LERSSGVICHSHYVENLVQNLVPNKPVRHIPMGIVIPAESVLDKAQARSLVKVDRGDF
                     IVASFGRITPSKRLEPALRAFARFRKQHPNSRYLLVGEAEPDYPIEAIVKSMGLSDCV
                     RFVGFSTLDDFERYIRASDVCLNLRYPTAGETSAGLLRIMAAGRPVLVSDCGSFSELP
                     DSVALKVPVGHQGREEELIFRYLTLLAEQPQIADAIGRNTRQHVLQEHPLDLAASRYR
                     EFISSLEQA"
BASE COUNT          308 a          326 c          314 g          254 t
ORIGIN      
        1 gcctgttcca gacttgagat gaattcgcgg taccgactgg cggccaggtc aagaggatgc
       61 tcctgaagaa cgtgctgtct cgtatttctg ccaatcgcgt ccgcaatctg tggctgctcg
      121 gcaagaagcg tcagatagcg aaagataagt tcctcttccc tgccctgatg gccgacgggt
      181 accttcaacg ccacagagtc cggaagctca ctgaaggagc cacagtcaga gaccagaacc
      241 ggccgacctg cagccatgat tcgaagaagg ccagccgaag tttctccggc ggtcggatac
      301 cgaagattca ggcagacatc cgaggctctt atgtaccgct caaaatcatc cagtgtgctg
      361 aatccaacaa atcgaacaca gtcactgaga cccatggact taacgattgc ctcgatagga
      421 tagtccggtt ccgcttcccc aacgagcaaa tacctcgaat tgggatgctg ttttcggaat
      481 cgggcaaaag ctcgaagcgc tggctccagc ctcttggatg gcgtgatgcg accgaaactg
      541 gcgacgatga aatctccgcg atcaactttc accagagacc gcgcctgcgc cttgtcgaga
      601 acactttctg cagggattac aattcccatg gggatgtgcc gcaccggttt gttcggaacg
      661 agattctgaa caagattctc gacatagtgg ctatggcaga tgacacccga acttctctca
      721 agcagtttcc ggttcagcgg atgctgcatg aaaagacgaa accgatcttg cgcggatgtc
      781 cctggtgcca ccatgcgccg agccagttcg cgtccttgtt gcagaccgta ctcatcgcac
      841 aactccgcct cgtaagcatc ataccgtcct cgatccagcg tgatgccggc aatgaagtga
      901 tgcaggataa aatcatgcag aaccacgatt ccaggaaaac gaagggctgt ctcgtagatt
      961 tcctcatgat ccaaaccatt gcccatctga tagagaagat gatcgtaagg ccgttcgcga
     1021 tgcgattttc taaaactctt gatcggccga atgtccagag actccaaacc tttcaagtcc
     1081 acatcgcgct gggtggcgaa aagcgtcatg tcaaagtgtg gcagaagatg ggggatcagt
     1141 tccttactgt aatcggaaat gcctgaatgc acagggggaa gcggactgaa aaacgcaaga
     1201 cg
//
LOCUS       DAOQUC010001457         1202 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_168678_length_1202_cov_2.655623, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001457 DAOQUC010000000
VERSION     DAOQUC010001457.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1202
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_168678_length_1202_cov_2.655623"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1033)
                     /locus_tag="PKH07_19090"
     CDS             complement(<1..1033)
                     /locus_tag="PKH07_19090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37104.1"
                     /translation="MASKALDKFLRPSSKTGQYTFICSPLRPPEGYVQQFREKLLKEC
                     SRTPGLLSLYEDRRTFYSGIGVLRLIGIFDPKLVNPSRGSLSQALETADPEGHIGRQV
                     LCVTPELFQSLRRIDCLLEPEKFWGQKYPIQALPDEQKRYQYVIRLMDLFVAGHLETM
                     VRAAVERVVNTADFLARSSQMCLFLDLVKDILREPSIPEWDEFQRGFQDLATKWNSRH
                     LKRYMDIMPWVPKALEIILDALERMDGYFVKSNIVNFKPGADAEAPQAAFVTENQAVA
                     FLCPWSPSRGLEVMLRLMKEHETFVDVLPATFALQLYEYCKGSGGFHKYVKSCFKMEA
                     MTGHMERPNL"
BASE COUNT          282 a          331 c          306 g          283 t
ORIGIN      
        1 tgaggttggg acgttccatg tgccctgtca tggcttccat tttgaagcag ctcttgacgt
       61 atttgtgaaa cccgccgctg cccttgcagt actcgtacag ttgaagcgca aaggttgccg
      121 gtagaacgtc cacaaaagtc tcatgctctt tcatcagtcg cagcatgact tccaaaccgc
      181 gagagggact ccaagggcag agaaaggcca cggcctgatt ttctgtgaca aaggccgctt
      241 gaggtgcttc cgcgtcggct cccggcttga aattgacgat attggacttg acgaagtagc
      301 cgtccattct ttccaaagcg tccaggatga tttcaagagc tttgggaacc catggcatga
      361 tatccatgta gcgtttcagg tgccgactgt tccacttggt ggccaggtct tgaaatcctc
      421 tctgaaactc atcccactca gggatggaag gctcacgcag aatgtccttg acgagatcga
      481 gaaacagaca catctggctg cttctggcaa ggaaatcggc agtattgacc acgcgctcca
      541 ccgctgcacg caccattgtt tccagatggc ctgccacaaa aagatccatc agacggatga
      601 catactgata tcgtttctgc tcatcgggaa gcgcctgaat cggatatttc tgtccccaga
      661 acttttcggg ttccagaaga cagtcaatcc ggcgaagcga ctgaaaaagt tccggggtca
      721 cacacaagac ctgacggcca atgtgacctt ctggatcggc tgtctccaat gcttgcgaga
      781 ggcttccacg cgacgggttg accagtttcg gatcgaatat cccgatcagg cgaagtactc
      841 cgatgccgct gtaaaaggtg cggcgatcct catacagaga caacagccct ggagttcgcg
      901 agcattcttt gagcagtttc tctctaaact gctgcacgta gccttcagga ggcctcagtg
      961 gcgagcagat gaatgtatac tgccccgttt tgctgctggg tcgcaggaat ttgtccagag
     1021 ccttggacgc catagttcgc cttcgattcc cttccgcctt cttacgccac taagccaatt
     1081 gaagatgacc gatggtcacc ctcagcgact ctcccatgtc tgaagcccac ctgtgtcgaa
     1141 gttgaaatca atgatgcgaa caccggattc ctcaagaacc cgacgcacgc ggtgagctga
     1201 at
//
LOCUS       DAOQUC010001458         1201 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_168828_length_1201_cov_3.872600, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001458 DAOQUC010000000
VERSION     DAOQUC010001458.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1201)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1201)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1201
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_168828_length_1201_cov_3.872600"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..420)
                     /locus_tag="PKH07_19095"
     CDS             complement(<1..420)
                     /locus_tag="PKH07_19095"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HNT37105.1"
                     /translation="MKVVVLSDTHLPAFGVRLPDEAIRAIGESDLILHSGDIVQKSFL
                     DELSGRKEVRAVLGNMDMEPLASLLPETRIEELEGVRIGMTHGFGAPQGIEQRVQTRF
                     RKEDNLDVLIFGHSHVPMQRMQGNLLLLNPGSATDRVY"
     gene            complement(433..810)
                     /locus_tag="PKH07_19100"
     CDS             complement(433..810)
                     /locus_tag="PKH07_19100"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNT37106.1"
                     /translation="MVDNIEGKSISRQLPKRSPALFSLTISVSEPSNSLLVRYEYREK
                     SAVPIYEYDCLKCRKRFDVFCARVGGESAGEKCPDCGSRRTRKALSLFGMSVRGSSNQ
                     PGSSGSKGRSCAGCAATSCATCR"
     gene            866..>1201
                     /locus_tag="PKH07_19105"
     CDS             866..>1201
                     /locus_tag="PKH07_19105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37107.1"
                     /translation="MLRKAFCGFRFLFIVGSVLSLVGIGLAGQSVCAETAIGFTDLGQ
                     VLLPDISAEQNPVWVSDQVYGVFHRYVQSLGAGLEPVGTDSLALIQSPADDPIGETPT
                     ITPTPQDTET"
BASE COUNT          285 a          354 c          282 g          280 t
ORIGIN      
        1 atacacccga tccgtcgcac tgccgggatt gagcaacaac agattcccct gcatcctctg
       61 catcgggacg tgtgaatgcc cgaagatgag cacgtccaga ttgtcttcct tccgaaaacg
      121 ggtctgaacc cgttgttcga taccttgcgg agcgccaaaa ccgtgcgtca tcccgattcg
      181 gactccttcc aactcttcaa ttcgagtctc aggaagcagt gatgcaagcg gctccatatc
      241 catattaccc aacaccgccc gaacttcctt cctgccactg agttcatcca gaaagctctt
      301 ctgcacaata tcgccgctgt gaagaatcaa atccgactct ccaatggctc ggattgcttc
      361 atcaggaagc ctgacaccaa aagccggcag atgggtgtcc gaaagcacaa ctaccttcat
      421 ggagatcccc gcctaacgac atgttgcgca gctggttgcg gcacatccgg cacaactgcg
      481 acctttggag ccggaactcc ctggttggtt gctcgaaccg cgaacactca tcccgaacag
      541 agacaatgcc ttacgcgtcc gacgactccc gcaatccgga cacttctccc ccgctgattc
      601 tccacccaca cgggcacaga agacatcaaa acgctttctg catttcaggc agtcgtactc
      661 gtagatcggc acagcactct tctcccgata ttcgtacctg accaagagag aattactagg
      721 ttcagacacc gaaattgtca aggaaaataa cgccggagag cgttttggaa gttgacggga
      781 gatgctcttt ccctctatat tatccaccat gtaggctgcg gtggttcgag gcgaacctgc
      841 ttgtcccaga acaggagaga tttcaatgtt gagaaaagcc ttttgtggat ttcgattttt
      901 gttcatcgtc ggatctgttc tttcccttgt gggcattgga ctcgccgggc agtccgtctg
      961 cgctgagaca gcgatcggtt tcacagacct cggacaggtc ttgcttcccg atatctcggc
     1021 tgaacagaac ccggtttggg tgtctgatca ggtatacgga gttttccaca gatatgtcca
     1081 gtcccttgga gcaggattag agcctgtagg gactgattcc ttggcactta tccagtcacc
     1141 agcggatgat cctattggcg agactcccac aataacgcca acccctcagg acaccgaaac
     1201 a
//
LOCUS       DAOQUC010001459         1201 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_168922_length_1201_cov_2.473822, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001459 DAOQUC010000000
VERSION     DAOQUC010001459.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1201)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1201)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1201
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_168922_length_1201_cov_2.473822"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1201)
                     /locus_tag="PKH07_19110"
     CDS             complement(<1..>1201)
                     /locus_tag="PKH07_19110"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HNT37108.1"
                     /translation="DYFVLGAVTDFYASQQTTASDALGRQRQKSALTMTVDARIVNTK
                     TGEILAANTVQIDEPKIVGSPNDALESRIEKMRQDMVRKAAKQLAFNVVNAVYPISIA
                     KVSGDKIYLNRGASLGMGVGDTLTVYTQGEAIMDPDTKRPLGYEETIVGDIVVDEVQE
                     RLAIASTISGTVKEGMLCRPVASSSESGVSEEADQEKGRGTLAILGFTKLDGISAEVV
                     EVVQTYFYTELGNRGQYPDLQVVERQQLEKLKLEMDLSEEGYLSPATAMRIGELTGAQ
                     YCLVGSIHQYAEESKESVLREDLRKTITEISFSMDCRVLETEKGEVIDTAIASERVKA
                     QSNVDEARSAVLRSAVRKACADLFGGVVSDNSAPVAEAGASDQTVSNDMNPEQGVPGA
                     FLVVSAFA"
BASE COUNT          265 a          344 c          294 g          298 t
ORIGIN      
        1 ccgcaaacgc gctgacaacc agaaaggctc cgggaacacc ctgttcggga ttcatatcgt
       61 ttgaaacagt ctgatccgag gctcctgcct cagcaacggg ggcagagttg tcggatacca
      121 caccaccaaa cagatcagcg cacgcttttc gaacagcgct tcgaagaacc gcagaccgcg
      181 cctcgtccac gttgctttgt gccttgacgc gctcggaagc gatcgccgta tcaatgactt
      241 cgcccttttc cgtctcaagc acccggcagt ccatggagaa ggaaatctcc gtaatcgtct
      301 tcctgagatc ctctctgaga acggattcct ttgactcttc ggcgtattga tgaatggagc
      361 cgacaaggca gtattgagcg cctgtgagtt caccgattct catggctgtt gcaggtgaga
      421 gatagccctc ttcactcaaa tccatctcca gtttgagttt ttccagctgc tgtcgttcga
      481 caacctgcag atcggggtac tggcctctgt tgccaagttc cgtgtagaaa taggtctgaa
      541 cgacttcgac gacctcggca gaaatgccat cgagtttcgt gaatcctaga attgccagcg
      601 ttccccgtcc cttttcctga tcggcttctt ctgacacacc ggattcagaa gaggaggcaa
      661 caggtcggca gagcattcct tccttgactg tgccggagat ggttgaggcg atcgccaaac
      721 gttcttgaac ttcatccaca acgatgtcgc ccacaatcgt ctcttcatag cccagaggcc
      781 tcttggtgtc cggatccatg atggcctctc cctgcgtgta gactgtcaat gtgtctccaa
      841 cgcccattcc aagacttgcc ccacggttca gatagatctt gtcgccgctg actttggcaa
      901 tagaaatcgg atagacggcg ttgacgacgt tgaatgccag ctgcttggct gcctttcgaa
      961 ccatatcctg acgcatcttc tcaattcggc tctcaagagc atcgttgggt gaaccaacga
     1021 ttttcggttc atctatctga accgtattgg cagcaagaat ctcccctgtc ttggtgttga
     1081 cgattcgagc atccacagtc atggtcaacg cagacttctg cctctgtctg cccagagcat
     1141 cggatgccgt cgtctgctgt gaagcgtaga agtcggtaac agcgccaaga acgaaataat
     1201 c
//
LOCUS       DAOQUC010001460         1199 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_169331_length_1199_cov_3.868881, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001460 DAOQUC010000000
VERSION     DAOQUC010001460.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1199)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1199)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1199
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_169331_length_1199_cov_3.868881"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..549)
                     /locus_tag="PKH07_19115"
     CDS             complement(<1..549)
                     /locus_tag="PKH07_19115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="catalyzes the reversible isomerization of glucose
                     6-phosphate to fructose 6-phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoglucose/phosphomannose
                     isomerase"
                     /protein_id="HNT37109.1"
                     /translation="MLQKVERFPRMLEEAYKIGADFDASQFGEIRQAVLFGMGGSAVG
                     GDFLRCCAYDQARIPISVVRGYTVPNSVDCHTLAILCSYSGNTEETLSCYEQAIQKKA
                     QILCITSGGKVEKLAKEHGHPLLKIPGGLPPRSALGYSFAPLLRVFERLGLTPNQENS
                     LREAVDVLDECVNLFGGTVLSDN"
     gene            842..>1199
                     /locus_tag="PKH07_19120"
     CDS             842..>1199
                     /locus_tag="PKH07_19120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37110.1"
                     /translation="MMALSVLLVLLMSPELGTAQQQSPAAFLSILYTGMAEANIEPCA
                     CSGQNLGGVVSRSLEIENVRSEGIPTLLLESGQAFASGTELDRARTEFYLEMLDRMGY
                     DAMNIATQDLVLGPEFL"
BASE COUNT          275 a          317 c          318 g          289 t
ORIGIN      
        1 gttgtcagac agcaccgtgc caccgaaaag attgacacac tcatcaagaa cgtccacggc
       61 ttcgcgcaag gagttctcct ggttgggagt cagaccaagc ctttcgaaaa ctctcagcaa
      121 tggggcaaat gaatatccca atgctgaacg aggtggcagt cctccaggaa tcttgagcag
      181 aggatgcccg tgctccttgg ccagtttctc caccttgccc ccgctggtga tgcagaggat
      241 ctgcgccttc ttctgaattg cctgctcata gcagctgagc gtctcttccg tattgccgga
      301 gtaggaacag aggattgcca gagtgtggca gtctacactg ttcggcacag tgtaccctcg
      361 aacaacggat atcggaatcc tggcctgatc ataggcacag cagcggagaa agtcgccccc
      421 gacggctgat ccgcccatgc caaagagaac cgcctggcgg atctctccga actgcgaggc
      481 atcaaaatca gcgccaatct tataggcctc ttcaagcatt cggggaaaac gctcaacttt
      541 ttgaagcatc cccgaaggat ctgcttcctt cattttttgg atatcgtcca aacaactctg
      601 atgcatgact gactatgacc tccgcatgat tggtacaaag acaaacccat actctccagg
      661 atgagccact tggctggacg tagtctagca tcaggggctt gtttgtcaaa gagattatgg
      721 gatggtcggt acccgagctt gctcgcagct tgcgctcaag tttcgcccca tgatataaga
      781 tgctctgaat gcggatgctg aggaaaccgg atgagttgtt tcctctgagg gtatttggaa
      841 aatgatggcc ttgtcagtgc ttctcgttct gctgatgtcg cccgagttgg gaacagcgca
      901 gcaacagtct cctgccgcat tcctgagcat tctctatacc ggcatggcgg aagccaacat
      961 cgagccttgt gcctgttcgg gtcagaacct ggggggtgtc gtctcgcgat cactggaaat
     1021 tgaaaatgtt cgaagcgaag gcattcctac actgcttctg gagtctggcc aggcttttgc
     1081 ctcggggact gaacttgacc gagctcgcac tgaattctat ctggagatgc ttgaccggat
     1141 gggatatgac gcgatgaaca tcgcaacaca ggatcttgtg cttggtcccg agtttcttg
//
LOCUS       DAOQUC010001461         1199 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_169368_length_1199_cov_2.770105, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001461 DAOQUC010000000
VERSION     DAOQUC010001461.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1199)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1199)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1199
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_169368_length_1199_cov_2.770105"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1199)
                     /locus_tag="PKH07_19125"
     CDS             complement(<1..>1199)
                     /locus_tag="PKH07_19125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37111.1"
                     /translation="QAIDLLQQLLTVIPGQRDASMLLAETLAGKGMVGEAARIFIALG
                     EQARSEKKLLEAVEYFKRVAVLTPDDAEIQLLLGSVLCDVGNREAAAEHWSVGLDRLL
                     HAQDWEGLFAALCPMRMLAEHNIQLSRKITASLEEIVSKWPQANKKPEVLKVWMDARL
                     NLLALLGNDIARVDVAQEIRALAARLVTEEEACAQAWEWLGRASETTDPKKASSLLKA
                     AALYGAQGEGERTRSLCDEVRALSCVSLQDRETLVALLFGAGEAEQAQREQLALAEGL
                     LDAGEVEAATAQFEKSLEHNPNQPLVLERLFDLYVQRSLTEKARQVAQMLSEHWEQEK
                     NILQAIAWQKRVLLPGREEKDEQQLMVTALTGLVKAELDCRIGQVLRLASLFESAGMH
                     LEKKATI"
BASE COUNT          256 a          377 c          276 g          290 t
ORIGIN      
        1 gatcgttgcc ttcttctcca gatgcattcc ggcagattca aagagagacg ccagcctgag
       61 tacctgacca attcgacagt caagttctgc cttcaccaaa ccggtgagag cagtgaccat
      121 gagttgctgt tcgtccttct cctctcgccc cggaagaagc actctcttct gccaggcaat
      181 ggcctgcagg atattcttct cctgttccca gtgctctgag agcatctggg caacctgcct
      241 cgctttttca gtaagactcc tctgcacata cagatcaaac aggcgttcca acaccaaagg
      301 ttgattggga ttgtgctcca gggatttctc aaactgagca gttgccgcct caacttcccc
      361 ggcgtcaaga agcccttccg ccagcgcgag ttgttccctc tgtgcttgtt ccgcttcacc
      421 tgcaccaaac aagagcgcaa caagcgtctc tcgatcctgc agtgatacac aggaaagggc
      481 acggacttca tcgcaaagag aacgtgttct ctcgccttcc ccctgtgcgc catagagtgc
      541 tgcggctttc aggagtgatg atgccttttt ggggtccgtc gtctcgctgg cacgtccaag
      601 ccattcccaa gcttgagcgc aggcttcttc ctcggtcaca agccgggctg caagcgccct
      661 tatctcctgt gccacgtcaa cacgtgcaat gtcatttccc agcaacgcaa gtaggttgag
      721 ccgagcatcc atccagacct tcaagacttc gggtttcttg ttcgcctgag gccatttgct
      781 cacaatctct tcaaggcttg cggttatctt gcggctgagt tggatattgt gctctgccaa
      841 catacgcata ggacagagcg ctgcaaacaa cccttcccag tcctgtgcgt gaagcagcct
      901 gtcgagacca acactccagt gttccgcggc cgcttccctg tttccaacat cacacaacac
      961 cgagcccaaa agcaattgaa tctcagcatc gtccggagtg agtacagcaa ctcttttgaa
     1021 gtactccact gcctcaagca gttttttctc cgatcgcgcc tgctctccaa gcgcgatgaa
     1081 aatcctggcc gcctctccaa ccatcccctt ccccgcaaga gtttcggcca ggagcattga
     1141 ggcatctcgc tgcccgggaa tcactgtcag caactgctgc agaagatcaa tggcttgtt
//
LOCUS       DAOQUC010001462         1199 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_169488_length_1199_cov_1.812063, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001462 DAOQUC010000000
VERSION     DAOQUC010001462.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1199)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1199)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1199
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_169488_length_1199_cov_1.812063"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..600)
                     /locus_tag="PKH07_19130"
     CDS             complement(<1..600)
                     /locus_tag="PKH07_19130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37112.1"
                     /translation="MNRQWIVPTEKTLLVIVLILLSLPANVYAAAPNDEDYNDDAGFT
                     SSSSQFTLDGIKYTLTGTPDTVVTNQGAGSGGSSLGNDGADYYISIYANSVKIEAADG
                     SYFKLNGFTMDVYADANVTIAPSSGTPLTRTCNGSILVENVNVSGNTDFQYITSVTFS
                     GSNMLLNLDDLDFSPPVPPTYTVTYNGNGNTGGSAPTDGS"
     gene            complement(852..>1199)
                     /locus_tag="PKH07_19135"
     CDS             complement(852..>1199)
                     /locus_tag="PKH07_19135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37113.1"
                     /translation="GLVYYGCCDPLDGKMNEVRMIPKVRKVSMSPWVNEERGAEEIAT
                     EFVYSRKPSPALLAWDHFNVDCVREDLLATRTVCDKYHCPLEFILKDISTVRYEPERL
                     TQWCKTAMEVVEG"
BASE COUNT          294 a          305 c          316 g          284 t
ORIGIN      
        1 actgccatct gtcggagcag accctcccgt gttgccattg ccattgtatg tgactgtgta
       61 ggttggtggg acaggtggtg agaaatcaag gtcgtccaga ttgaggagca tgttagaacc
      121 ggaaaaggta acgctggtga tatattgaaa atccgtattg cccgacacat ttacattctc
      181 aaccagaata cttccattgc aggtacgggt cagaggtgtc ccactactgg gtgcaatggt
      241 tacattggcg tctgcataga cgtccatggt aaaaccattc aacttgaagt agcttccgtc
      301 tgcggcttcg atcttgacgc tgttggcata gatggagatg taatagtccg ctccatcatt
      361 cccgagcgac gaaccccctg aacctgcgcc ttgatttgtg acaaccgtat ccggagtccc
      421 cgtcaaggta tacttgatcc catcgagagt gaactgagat gacgaactgg taaatccagc
      481 gtcatcattg taatcctcat cattgggcgc tgcagcgtag acgtttgccg gcagggacag
      541 aaggatcagc acaatcacaa gcagagtctt ctcggtcgga acaatccatt gtctgttcat
      601 cttcttactc ctaaatgggt catcaatata tcgcattgta tatcatgccg gcaggatctg
      661 cacaatgaat ccctatccca gactcctcac cagtcccaca agcaggggtt ttggagaacc
      721 tgagaaggga agaaacgtaa ccagaggagg ctgaccggct aagtcggaaa cggacagccg
      781 ggaggaagga gtgatagtcg tgggaacgac gaaaggagtt ctttgaatgg gcaccccgag
      841 acgagcagtt ctcatccttc aaccacttcc atcgccgtct tgcaccactg cgtaaggcgt
      901 tcgggttcat agcgtacggt gctgatgtcc ttgaggatga actccagtgg gcagtgatac
      961 ttgtcgcaga ctgtgcgagt tgccagaaga tcctcccgaa cacagtcaac gttgaagtga
     1021 tcccaggcga gcaatgcagg actgggcttg cgtgagtaaa cgaactctgt tgcgatctcc
     1081 tcagcgcctc gttcttcgtt cacccatggg ctcatggaga ccttgcgcac cttggggatc
     1141 atgcgcactt cgttcatctt tccgtcgagc ggatcacagc aaccatagta gaccaggcc
//
LOCUS       DAOQUC010001463         1198 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_169593_length_1198_cov_3.125984, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001463 DAOQUC010000000
VERSION     DAOQUC010001463.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1198
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_169593_length_1198_cov_3.125984"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(41..1039)
                     /locus_tag="PKH07_19140"
     CDS             complement(41..1039)
                     /locus_tag="PKH07_19140"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNT37114.1"
                     /translation="MQYVVFGNTGMLVSRFTLGGMTLGTRFEPDQATQVVDEAIDHGV
                     NFIDTADSYRDSEECIGRALTPEKRDKVFLATKVFRTFCRNGKVGRNSRVNIINSLER
                     SLKVLKTDYVDLYQLHHPDEQTPVEETLSTLDQMVKQGKIRYVGVSNHYAWQMALMLG
                     ESKANHWESIVSFQASYSILDRQIEMESLPFCTKLNIAIMCYGPLCGGILTGKYQGLK
                     EIPEDSRAAGNRRTRDYLETADVRKIVERLEEIARDNDLKLNQLAILWLLGKSHATTV
                     ILGGSKSEHFTQIYEIADRTLPECVVKEIDDLSSCRIYCPFANQPFRMGPGLRKTF"
BASE COUNT          270 a          302 c          305 g          321 t
ORIGIN      
        1 ggagtgggca tagcccacat gcctgcaggt gctaacctgc tcagaaggtt ttcctcaggc
       61 ctggtcccat tcggaagggt tgattggcaa aaggacaata gattcggcat gaggaaagat
      121 cgtcaatctc cttgacaaca cactccggaa gagtcctgtc agcgatttca tagatctgag
      181 taaaatgttc ggatttggag ccacccagaa tgactgttgt cgcatgtgat ttgcccaaca
      241 accagagtat tgccaactgg ttgagtttca gatcattgtc ccgcgcaatc tcttcgagac
      301 gttcgacgat cttccggacg tctgcagttt cgagataatc acgggttctt ctgttgcctg
      361 ccgctcggct gtcttccgga atttccttaa gtccctggta cttgccggtg agaatgccgc
      421 cacacaaggg tccgtagcac atgatcgcaa tgttgagctt tgtgcagaaa ggcaatgact
      481 ccatttcaat ctgtcgatcc aggatgctgt aactggcctg aaatgaaacg atagattccc
      541 aatgattggc tttgctttcg ccaagcatca gagccatctg ccaggcgtag tggttggaga
      601 cgccgacata gcggattttg ccttgcttga ccatttggtc aagcgtggag agagtctcct
      661 caactggggt ctgctcgtca ggatgatgaa gttggtaaag atccacataa tccgtcttca
      721 gcactttcag actgcgttcc agggagttga tgatattcac gcggctgttt cttccgacct
      781 tgccattgcg gcaaaaggtt ctgaagactt tcgtggcaag gaaaaccttg tcgcgctttt
      841 caggcgtcag tgccctgccg atacactctt cactgtcgcg gtaactgtct gccgtatcaa
      901 tgaagttgac tccatggtca attgcctcat caacaacttg tgtggcttga tccggttcaa
      961 agcgtgttcc caaggtcatt ccaccgagag tgaatcttga gacaagcatc cctgtattgc
     1021 cgaagacgac gtactgcatt tccgttactc ctaagagact gcctgcggtt ttatgtactc
     1081 cctgctgagt ctatcagact tggacgatcg ccttgatcac tccgtcgctg taggacgagg
     1141 cggtggtcaa gagtctttca gaatcttcga aacgcccctg gtggctgacc agggaact
//
LOCUS       DAOQUC010001464         1198 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_169604_length_1198_cov_3.006124, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001464 DAOQUC010000000
VERSION     DAOQUC010001464.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1198
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_169604_length_1198_cov_3.006124"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..684)
                     /locus_tag="PKH07_19145"
     CDS             complement(<1..684)
                     /locus_tag="PKH07_19145"
                     /inference="COORDINATES: protein motif:HMM:NF027401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4815 domain-containing protein"
                     /protein_id="HNT37115.1"
                     /translation="MMSCGLVTVVCFCCFGLLSVSAFADTQKISTVGVNPFVLEGLDL
                     TVEGRQLTVSCGICRVGRKKVVVKEPAVFTVDPPDDVQVSGEKLTLRLEQPQGWFRGT
                     RLKEFIAGGTSLPGCFSPGSLILKLEDGSILKESEDYLLDSEWGMLGRTENGRIADTT
                     ALSADYRYSLMRLDTLFVDENGVVSLKQGESRMTCPHPPEKRPDATALANIFMPYRTK
                     VVEPWQVFVI"
     gene            932..>1198
                     /locus_tag="PKH07_19150"
     CDS             932..>1198
                     /locus_tag="PKH07_19150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37116.1"
                     /translation="MNWRRFIPWILVVIVNILLAIYLFQSYRASHTTEDMTSGEHRAE
                     ESQISTSPQSQLPQVTPTQAAQIQTADSDTDQDGRSGQRASLAKA"
BASE COUNT          280 a          344 c          297 g          277 t
ORIGIN      
        1 tatcacaaaa acttgccatg gctcaaccac tttggttcgg tagggcatga agatattggc
       61 cagtgccgtt gcgtcagggc gcttctctgg cggatgcgga cacgtcatcc tcgactctcc
      121 ttgcttcaaa gaaacaacac cgttctcatc aacgaagagc gtatctaggc gcatcaggct
      181 gtaacggtaa tcagcagaga gtgccgtcgt gtcggctatc cttccgttct cggttcgacc
      241 cagcattccc cactcggagt ccagaagata gtcctctgat tccttcagaa tggagccgtc
      301 ctcgagtttc agaatgagcg agcccggtga aaaacaaccc ggcagggatg ttccaccggc
      361 gatgaattcc ttgaggcgag tccccctgaa ccacccttgg ggctgttcca gtcgcaatgt
      421 aagtttctcg ccgcttacct gaacgtcatc gggagggtct acagtgaaaa cagcgggttc
      481 tttcactaca accttcttgc gacccactcg acatataccg caactgacgg tgagttgcct
      541 gccctcgaca gtcagatcaa ggccttcgag aacaaagggg ttgactccta cggtggatat
      601 cttctgcgtg tctgcgaacg ccgagacact cagaagaccg aaacagcaga aacacacaac
      661 ggtgaccaga ccgcaactca tcatcgaact cctttgggac actgacacct gtcaaggcat
      721 catgaactgg tacgggaggt actatcccac aggtgaggtt ggaggacaaa tccatccgcc
      781 atcctgtcgc tttgtctttg ttttcataag ttgccctctg ggttcgtgtg cgtataatag
      841 attagggtca gaggtcgcgt ccgttttgtc cgctgcgtct gtccatcttg cagaaacagc
      901 atgacccaat ccgacctgga ggtacccaga aatgaactgg cgacgtttta tcccttggat
      961 tctggttgtt atcgtcaata tcctgttggc catctacctc tttcagagct atcgtgcttc
     1021 acacaccaca gaggatatga cgagtggtga gcaccgcgcc gaagagagtc agatttccac
     1081 ctccccacaa agccagcttc cccaagtcac tccaacgcag gcggctcaga ttcagaccgc
     1141 agattccgat accgatcagg atggccgttc cggccaacgt gcatccttgg ccaaggcg
//
LOCUS       DAOQUC010001465         1198 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_169613_length_1198_cov_2.919510, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001465 DAOQUC010000000
VERSION     DAOQUC010001465.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1198
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_169613_length_1198_cov_2.919510"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..864)
                     /locus_tag="PKH07_19155"
     CDS             complement(<1..864)
                     /locus_tag="PKH07_19155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37117.1"
                     /translation="MSLFISPVQQNLLFFVIATLWLFVSLPAEADINVALSENGAIAM
                     ADSQYADFGVVKAIDGQWISFGDPAQNNRWHSSLSEPHPHWVWIQFKQKARINRVVVR
                     PADPIDVPIDLAGEYRVLGELSMREIFHARNLKPEPDKYFYEFTFEPVVTNNFRLRID
                     RSSHEPYPNSAQLSEIAVFGSFEEDHRPELSQKFSELKPCLAPTDIKQLRIVQSDSEV
                     VFRSDWIQVNLSREQARMDYLCWDSLGKGKLDTNLVKPGADGGVQISLPSVPALTLDR
                     CHVEGNVLRYEM"
BASE COUNT          291 a          315 c          301 g          291 t
ORIGIN      
        1 catttcatat cgcaacacat tgccttctac atggcagcga tcgagcgtca gagcaggaac
       61 ggaaggaaga gaaatctgaa ctccaccgtc agcgccgggc ttgacgagat ttgtgtccag
      121 cttccctttg cccagactgt cccaacaaag atagtccatt cgggcttgct ctcgcgacag
      181 gttcacctgt atccagtctg agcgaaaaac cacttccgaa tcactctgca ctattctcag
      241 ttgtttgatg tccgtcggcg ccaaacacgg tttcaactca gagaacttct gactcagctc
      301 cggccgatgg tcttcctcaa acgaaccgaa gactgcgatt tcggacaatt gtgcgctgtt
      361 gggatacggc tcgtgagacg atcggtcaat tctgagcctg aaattgtttg tcacgacagg
      421 ctcgaaggtg aactcataga agtacttgtc cggttccggc ttcaggttcc ttgcatggaa
      481 aatctcgcgc atcgagagtt cgcccaagac cctgtattcc cctgccagat caatgggcac
      541 atctatcgga tcagcagggc gcaccaccac ccgatttatg cgtgctttct gcttgaactg
      601 aatccagacc cagtgaggat gcggttcgga caatgacgaa tgccaacggt tgttctgtgc
      661 aggatcgccg aaggaaatcc attgcccatc tattgccttc acgacaccaa aatccgcata
      721 ctgagagtcc gccatggcga tggctccatt ttcgctcaga gcaacgttga tatctgcctc
      781 tgctggcagg gatacaaaca gccagagtgt cgcgatcaca aagaacagta gattctgttg
      841 aacgggagag atgaagaggg acatatgctt gcctccaatc gtggctccga aggatctgtg
      901 gcgtgcagtg tatcacgctg gatatggact gcaattgcgg gcagtttttg tttgaaagga
      961 aaggcggagt cttcgtgggc atgtcttgtg cctcgcaatc gctgccagag cagcagcggt
     1021 tacaagaatc gcaaccagag cacaagaaag gtggaatcct gctaagctat ctatcgctcc
     1081 tttcactccg cttcacactt gcgtttctat cggtgacttt cgcaacaatg acaatccgat
     1141 tagcgtccgt cagatcaaga atgcgcctag tgttcatgaa ttcggtcgga tttacgta
//
LOCUS       DAOQUC010001466         1198 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_169642_length_1198_cov_2.681540, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001466 DAOQUC010000000
VERSION     DAOQUC010001466.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1198
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_169642_length_1198_cov_2.681540"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..829)
                     /locus_tag="PKH07_19160"
     CDS             complement(<1..829)
                     /locus_tag="PKH07_19160"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HNT37118.1"
                     /translation="MGASEQTLLIDADFPGGNIVVERIEGDDCFVHQDLRDTTRDWFY
                     WCFRVRNAQGRRLTFHFTQSNVLGVEGPAVSLDQGVTWRWLGDSCIQEKAFSYSFGLD
                     EREVRFSFGMPYLEEHLARFLTDHRQNQFLRTETLCTSRKGRTVARIHIGCIHREPVA
                     RVLLTCRHHCCEMMASYSLQGLMQAVISNSEEFAWLRNNVEFLVVPFVDKDGVEDGDQ
                     GKMRHPHDHNRDYLGEPADSIYPEVRALREFVPKWSGGLLRVGLDLHCPYIRGAHNEV
                     "
     gene            complement(951..>1198)
                     /locus_tag="PKH07_19165"
     CDS             complement(951..>1198)
                     /locus_tag="PKH07_19165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNT37119.1"
                     /translation="SSLISDEEKEKILAPDNSEADNIAQDPKAVGIRGHMIQIQDLVN
                     AVNENRDPMVTGEQARSAVDLILAIYKSSQTGREADV"
BASE COUNT          285 a          342 c          304 g          267 t
ORIGIN      
        1 tgacttcatt gtgagcgccc cgaatgtaag gacagtgaag atccagtcca acacggagca
       61 gaccgcctga ccactttggg acgaactccc tcagtgcgcg cacttccgga tagatgctgt
      121 cagcaggctc acccaagtag tccctgttgt gatcgtgagg atgacgcatc ttgccctggt
      181 cgccgtcctc cacgccgtcc ttatctacaa acggaacaac gaggaactct acgttgttgc
      241 gcagccaggc gaactcctcg ctgttggaga tcacggcctg cataagacct tgcaggctgt
      301 aacttgccat catctcacag cagtgatggc gacatgtcag cagaacacgg gcaacgggtt
      361 ccctatgaat gcatccgatg tgaatgcgcg cgacggttct tcccttgcga cttgtgcaaa
      421 gtgtttccgt cctgaggaat tggttctgcc tatgatccgt gagaaaccgc gccagatgct
      481 cttcgagata gggcattcca aaactgaaac gcacctctct ttcatccaga ccgaaggagt
      541 aggagaaggc cttttcttga atgcaggagt ctccgagcca gcgccaggtc actccttgat
      601 ccaaactcac cgccgggcct tcgacaccga ggacattgct ttgtgtgaag tggaaagtca
      661 gtcgccgccc ctgcgcgttg cgaactcgaa agcaccagta gaaccaatcc cgtgttgtgt
      721 cgcgtaagtc ttgatggacg aagcaatcat ctccttcgat gcgttcgaca acaatgtttc
      781 cgccggggaa atcggcgtca ataagaagag tctgttcgga agcacccatt gaactctcac
      841 aagaagaacc ttcctgctgt cttagggacg cagaaaaaca acccactaac gtggagacaa
      901 gaaccgatat ccaaaacaca ccgccgaacc caaccgacga gcgcaatggt ctagacgtct
      961 gcttcgcgtc cggtctgcga agatttgtag atcgccagga tcaagtcaac ggcactgcgt
     1021 gcctgctcgc cagtgaccat tgggtcacgg ttctcattga cagcgttgac caagtcctga
     1081 atctggatca tgtgaccgcg aattccaacc gcctttggat cctgagcgat gttgtctgcc
     1141 tcactattgt cgggagcaag tatcttctct ttctcctcgt ccgagatgag ggaactac
//
LOCUS       DAOQUC010001467         1197 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_169875_length_1197_cov_2.801226, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001467 DAOQUC010000000
VERSION     DAOQUC010001467.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1197
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_169875_length_1197_cov_2.801226"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..887
                     /locus_tag="PKH07_19170"
     CDS             <1..887
                     /locus_tag="PKH07_19170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37120.1"
                     /translation="SFREAICKGKDYIEGSVLAEVFWYHTRYVVRSALFQAIKGLNLQ
                     RNAQGCLPVRILMLGGDDLLLVLKGSDAFRFIVAFAQKAKEMSNKSKEAWKPLNSGIA
                     EKLPEYAFGAGIALCPLNYPFHLAHQLAEQLLKSAKRMSREFSTVDWEVVVGSMVGDL
                     DDYRHNVYCRKMNKEQLKLIKRPLAVLPSNGHFDLKTLNDDVGELDKVCGEGEGKVAR
                     SKLRTFMDELGKGRRHAEWTARDFFGEHMGKPIIKKHFHAVWTESASKKYETQCFDLI
                     QMMEFIAKAEEAKDVQHA"
     gene            871..>1197
                     /locus_tag="PKH07_19175"
     CDS             871..>1197
                     /locus_tag="PKH07_19175"
                     /inference="COORDINATES: protein motif:HMM:NF015728.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RAMP superfamily CRISPR-associated protein"
                     /protein_id="HNT37121.1"
                     /translation="MSSTHKTIILKLKVETLEPFHIGDGMNLPGLYDSGQVCDSEGYP
                     MVPDDTVRGLVRDAASRLKNRLADFGIGSQETSFKWYSSEEGVDRVFNEIFDFLSGDG
                     MLLTSWR"
BASE COUNT          325 a          255 c          326 g          291 t
ORIGIN      
        1 agtcctttcg agaagccatc tgcaaaggca aggattacat cgaaggatca gtcctggcag
       61 aagtcttctg gtaccataca cgctacgtgg tgaggagtgc cctctttcag gcaatcaagg
      121 ggctgaatct gcagcgaaat gcgcagggtt gcctgcctgt gcgaattctc atgctcggcg
      181 gtgatgatct tctccttgtc ctgaagggat cagacgcgtt cagatttatc gttgcatttg
      241 ctcagaaagc aaaggagatg agcaataaga gcaaagaagc atggaaacct ttgaattctg
      301 gcattgccga aaaactccct gaatatgctt ttggcgctgg aatcgcctta tgtcctttga
      361 actatccctt ccatctcgca catcagctag ccgaacagtt gctgaaatct gccaagcgaa
      421 tgagccgtga attcagcacg gtggactggg aggttgttgt cggaagcatg gtgggagatt
      481 tggatgatta ccgtcacaac gtctattgca ggaagatgaa caaagaacaa ctgaaactga
      541 tcaagaggcc tcttgccgtt ctaccgagca atggacattt cgatctaaag acactgaacg
      601 atgacgttgg cgaattggac aaggtttgcg gagaaggtga aggaaaggtt gctcgttcca
      661 agctcagaac attcatggat gaactcggaa aagggcgtcg tcacgcagaa tggacggcaa
      721 gggatttctt cggagagcat atgggcaaac caatcatcaa gaagcacttt catgcagtgt
      781 ggacagagtc tgcatcgaaa aagtacgaaa cacagtgttt cgacttaata cagatgatgg
      841 agttcatcgc aaaggcggag gaggctaaag atgtccagca cgcataaaac gatcattctc
      901 aagctgaagg tagaaactct tgaaccgttc cacattgggg atggcatgaa tcttcctggt
      961 ctctatgatt ccggacaggt gtgcgattcc gagggctacc caatggttcc ggatgacact
     1021 gttcgcggac ttgtgcgcga tgcagcgtct cgactcaaga ataggttggc agattttggc
     1081 attggatcac aggaaacctc cttcaaatgg tattcgtccg aagaaggggt tgatcgtgtc
     1141 ttcaatgaga tctttgattt cctatcaggt gatgggatgc tcttgacaag ttggcgg
//
LOCUS       DAOQUC010001468         1197 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_169970_length_1197_cov_2.028021, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001468 DAOQUC010000000
VERSION     DAOQUC010001468.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1197
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_169970_length_1197_cov_2.028021"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..869
                     /locus_tag="PKH07_19180"
     CDS             <1..869
                     /locus_tag="PKH07_19180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37122.1"
                     /translation="LYERHEQRAIQSQYLFYMHLPVRLDPMRLHAFKSILQTPHPHRI
                     NRDLHPLAFYHAMLLWSKQMQQVVSRRLTVLLAHREAFLSSLAVATLLAGLLARTWGN
                     RRIRTVLIVMGFGLCEMGFSVLLILAFSAQLGYVYSRIGLLSTAFMFGTGLGAWIQTR
                     SSSVSSKASSVLIRNCCLFAVMIVLTPLVIKFGETRLSTMLHEIVFYALSFAAGLLGG
                     QVFPLALRSVSGAHGNDAEPQAGLFYGADLVGSALGAFGVSLLLLPLGGIWIVCLGMA
                     LVLGICVWVQRV"
     gene            complement(893..>1197)
                     /locus_tag="PKH07_19185"
     CDS             complement(893..>1197)
                     /locus_tag="PKH07_19185"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNT37123.1"
                     /translation="GNVMVGLVCPTSDVSGGGAKVISAMEMQIGTELPLSLKGLLRAI
                     GAKVSKAEANGIVRRCEQMGNQWAYGLQFDKLDGEIRREIMKWCLSGSWEEVPIVE"
BASE COUNT          221 a          342 c          317 g          317 t
ORIGIN      
        1 ggctttacga aagacatgag cagcgcgcta tccaatcgca atacctgttt tacatgcacc
       61 tgccggtgag actcgatcca atgcgtctgc acgcgttcaa aagcattctg caaacacctc
      121 atccacatcg gatcaacaga gatcttcatc ctctggcttt ctatcatgcc atgctcttgt
      181 ggtcgaagca gatgcagcaa gttgtctcca ggagactaac tgttctcctg gcgcatcggg
      241 aagcctttct gtcctctctt gcggttgcga cactgctcgc cgggctgctg gccagaacat
      301 ggggcaaccg cagaattcgg accgtgctga tcgtcatggg ttttggtttg tgtgagatgg
      361 gcttctccgt attgctgatt ctcgccttca gcgcccaact cggctacgtc tacagtcgaa
      421 tcggactgct cagcacagcc tttatgtttg gcacggggct tggcgcttgg atccagacca
      481 ggagcagttc cgtttcttct aaagcttcca gcgttctgat caggaattgt tgcttgtttg
      541 cggtgatgat tgttctgacg cccttggtga tcaagtttgg cgaaacccgt ctttcaacga
      601 tgctgcacga aatcgtcttc tacgcgcttt cattcgcggc cggcctgctg ggaggtcagg
      661 tcttcccgct ggctctgagg agcgtgtctg gcgcacacgg caatgacgct gaaccgcagg
      721 caggactctt ctacggtgcg gatctcgttg gatcagccct tggagcgttt ggcgtcagcc
      781 tgctcttgct gcctctcgga ggaatctgga tcgtctgttt ggggatggca cttgtcttgg
      841 ggatctgtgt ctgggttcag agagtctagt cagagacaca atggcgtctt cgtcattcga
      901 cgatgggaac ctcttcccag ctcccggaca gacaccactt catgatctct cgcctgatct
      961 ctccgtcaag cttgtcgaac tgcaggccgt aagcccactg gttgcccatc tgctcacatc
     1021 tccggacaat tccgttggcc tccgccttgg acaccttggc tccaatggct cgaagcagcc
     1081 ctttgagaga cagtggcagt tctgtgccga tctgcatttc cattgcggat atgactttgg
     1141 cacctcctcc actgacgtcc gatgtggggc agaccaaacc taccatgaca ttgccag
//
LOCUS       DAOQUC010001469         1197 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_169980_length_1197_cov_1.936077, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001469 DAOQUC010000000
VERSION     DAOQUC010001469.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1197
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_169980_length_1197_cov_1.936077"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..753
                     /locus_tag="PKH07_19190"
     CDS             <1..753
                     /locus_tag="PKH07_19190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37124.1"
                     /translation="HDYWTRRLRECRALQEHPLLGLLVRVDWLYRLGDYTNSAVLLCS
                     CVDSFRMELVLVYLNCKNTPGGGIKGHFCEWQKTVIQKALSEANKKKEKSKRQFAQDD
                     LLRSPVAVDSILNSWKDNWSHKKEMRSWPESPDDVQKSLAFAAELRDFRRQQGHLDTL
                     AEHRNLYIHTGKPFGRDEIDKALQYDGLMGGTFPSPWYSSLVRRFLSDPCVWRGSQAP
                     QGGEWQKKPWTEALASEIRGKLHEYGWLEAMR"
     gene            809..1177
                     /locus_tag="PKH07_19195"
     CDS             809..1177
                     /locus_tag="PKH07_19195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37125.1"
                     /translation="MNELSQAEFLSGLFQSDSELKDVKIFSYRRDFSFLWGLDASSEF
                     DLKTAKGLFVVVWDGMPESDSRGEVIDTAQYLTPFDWAVSAYLHAGEVPPDWKILIYK
                     TINDDESAPAFDVLGHFSGQ"
BASE COUNT          285 a          267 c          354 g          291 t
ORIGIN      
        1 cacgactact ggacgagaag gctcagggaa tgcagggctt tgcaggagca tcctctgctt
       61 ggactcttgg tgcgtgtgga ctggctctat cgtctgggag actacaccaa cagcgcggtt
      121 cttctgtgca gttgcgttga cagtttcagg atggaactcg ttttggtcta tctcaactgc
      181 aagaacactc ctggtggagg gatcaagggt catttctgcg aatggcaaaa gacggttatc
      241 caaaaggctc tttccgaagc aaacaagaag aaagagaaat caaagaggca gttcgctcag
      301 gatgatctgt tacgctcacc tgttgctgtg gacagtattc tcaactcgtg gaaggacaac
      361 tggtcacaca agaaggagat gcgatcatgg cctgagtctc cagatgatgt tcagaagtct
      421 cttgcttttg cggcggaact tcgtgatttc cggcgtcagc aggggcacct ggacaccctt
      481 gcggaacatc gcaatcttta catccacact ggaaagccct tcggacggga cgagatagat
      541 aaggctctgc aatatgacgg attgatgggt gggacgttcc cttcaccgtg gtactcttca
      601 ttggtcagaa ggttcttgag tgatccgtgt gtctggcggg ggagtcaagc accccagggc
      661 ggggagtggc aaaagaagcc gtggacagaa gctttggcga gcgaaatccg agggaaactg
      721 catgaatatg gctggctgga ggcgatgcga tgagggagat cccgcgcaac acactcgaaa
      781 ggagagattg tgaaaaggag ttgtcctgat gaatgagttg tcccaggcag agttcctctc
      841 gggattgttt cagtcagaca gcgagctgaa agatgtgaag atcttctcct acaggcgcga
      901 tttcagtttc ttgtggggac tggacgcttc ttcagaattt gacctgaaga cagcaaaggg
      961 tctatttgtc gtcgtctggg atggaatgcc ggagtcggat tctcgcggtg aagtcattga
     1021 cacggcacag tatctgaccc cttttgattg ggctgtttca gcctatctcc acgcgggtga
     1081 agttccgcct gactggaaga ttcttatcta caagacgata aacgatgatg agagcgctcc
     1141 cgctttcgat gtcctggggc atttctccgg ccaataattt ggccgtgaag atcggtt
//
LOCUS       DAOQUC010001470         1196 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_170071_length_1196_cov_3.355828, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001470 DAOQUC010000000
VERSION     DAOQUC010001470.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1196
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_170071_length_1196_cov_3.355828"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            151..510
                     /locus_tag="PKH07_19200"
     CDS             151..510
                     /locus_tag="PKH07_19200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013221038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF190 domain-containing protein"
                     /protein_id="HNT37126.1"
                     /translation="MELPKDAALLRVFIGESDQHSRRPLYQAIVEKAREYGMAGATVV
                     RGIMGFGKNTRIHTARILRLSEDLPIVVEIVDKPERIQAFLPELDTMVGDGLVTLEHI
                     QVITYRHRPDSSGSKKA"
     gene            859..>1196
                     /locus_tag="PKH07_19205"
     CDS             859..>1196
                     /locus_tag="PKH07_19205"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HNT37127.1"
                     /translation="MSVVRFPKRVPLSIVPTQIHHLPRLTRLLGGPEIFMKRDDLTGC
                     AMTGNKVRKLEFLCAEAIRRGATTLMTCGGVQSNHARTTAVAAARLGMKSILFLRGQC
                     PQTTQGNLLL"
BASE COUNT          294 a          310 c          322 g          270 t
ORIGIN      
        1 caaccttcgt ctcggaaaca agccaattcc ttatggagac ggaagtgctc ctcgcccttg
       61 ccaacatggc cattcaggtt cttctcggaa ttgccgtctt ttacctcggt ctgtccgttg
      121 gacgcatcat ctgagaaagg agcacaggcg atggaacttc caaaggatgc agcattgctt
      181 cgagtattca taggagagag cgatcagcac tctcgtcggc ccttgtacca ggcaattgtt
      241 gagaaggcgc gcgagtatgg gatggcggga gcaacggtcg tacgaggcat catgggcttt
      301 gggaaaaaca cgcgcatcca tacagcgcgg atactccggc tgtcggaaga tctgcccata
      361 gttgtggaga ttgtggataa acccgaacgc atccaagcct ttcttccgga actggacaca
      421 atggtcggag atggtttggt taccctggag cacattcagg tcatcacgta caggcacaga
      481 ccggactcct caggatccaa aaaggcgtag aatgttctca gagcattgcc gtgctcaatc
      541 ttagaaacaa cacagattgc gatccaaggc caaagtttga agaggaaatg caaccctgca
      601 aagatggctc ctagtgacac aaagtccgaa aggcgcgtat cattcattga gcaagcgtcc
      661 gaaggagtgg gcatagccca cacgcctgca ggtgctaacc tgctcggaat gatgaagggt
      721 tggtgttcag gacttctctc aaacgtcctg ctgccttctt tacccgagga acccattgtg
      781 ctacaaagaa accaaaacac ggcattgttt tccgtgtgaa tgagttttca aaccaggtta
      841 gtcgcaggag aagtgccgat gagcgtggtg cgattcccca aacgtgttcc cctctctatt
      901 gttccaactc agattcacca tctgcctcgc ttgacgagac tccttggtgg tccagagatt
      961 ttcatgaagc gtgacgatct gactggctgt gcgatgacag gcaacaaggt ccgaaagctg
     1021 gaattcctgt gcgcggaagc gattcgtcga ggtgccacca ctctgatgac gtgcgggggt
     1081 gttcaatcga accatgcccg aacgacggcg gtcgcagcgg cacgtctggg catgaagtcc
     1141 attctctttc ttcgtggaca atgcccccaa acgactcagg ggaatcttct cctgga
//
LOCUS       DAOQUC010001471         1196 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_170082_length_1196_cov_3.117441, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001471 DAOQUC010000000
VERSION     DAOQUC010001471.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1196
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_170082_length_1196_cov_3.117441"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..456
                     /locus_tag="PKH07_19210"
     CDS             <1..456
                     /locus_tag="PKH07_19210"
                     /inference="COORDINATES: protein motif:HMM:NF014232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HNT37128.1"
                     /translation="KHISVHLCHECAEQQGESGSPSALLMNYLSLASLAQEKVKVATG
                     QKKVKPAQPSQACTGCQKTLEDFQETGRLGCAQCYKTFEKPLGEIIEKVQKSKQHRGK
                     VPEISSTTYRTQQELSECKHRLRQAVGNEEFEEAARLRDRIRGMEESLL"
     gene            560..>1196
                     /locus_tag="PKH07_19215"
     CDS             560..>1196
                     /locus_tag="PKH07_19215"
                     /EC_number="2.7.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0046314 - phosphocreatine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP--guanido phosphotransferase"
                     /protein_id="HNT37129.1"
                     /translation="MFPEQVQFNANKWLRATGDLADVVLTSRIRLARNLQDVPFTRWA
                     LPDDLASVTARFRRVASGTAELAGMAVIDFPRLDALEKEFLCERHLISREFVEGKKES
                     LLTLNSDETISIMVNEEDHLRIQAIRSGVDLQAAWEELNAIDDVLEKGMRFAFDERWG
                     YLTACPTNIGTGMRCSVMLHLPGMVLTQRIDKVLSAAAQLNLAVRGFFGEGS"
BASE COUNT          294 a          296 c          339 g          267 t
ORIGIN      
        1 aaacacatct cagtccacct ttgccatgag tgtgccgaac aacagggaga atccggttct
       61 ccaagcgcgc ttctgatgaa ttacctttcg ctagcctcac tggctcagga aaaggtcaag
      121 gtggctaccg gacagaagaa agtcaagccg gcgcaaccgt cacaggcttg tacaggctgc
      181 cagaaaactc ttgaggactt ccaggaaacc ggccgactcg gctgtgccca atgctacaag
      241 acttttgaaa aaccattggg cgagattatc gagaaagtcc agaaatcgaa acagcacagg
      301 ggaaaggttc cggagatcag cagcaccaca taccgaacac agcaggaact ctctgagtgc
      361 aaacaccgcc tgcgacaggc ggttggaaat gaggagtttg aagaagccgc gcgccttcga
      421 gaccgaattc ggggcatgga ggaatcactc ctctgaagtt cccttcccct acaagagctg
      481 tgaagatgtg taaggggtcc aggccgctgc aaagagttcg attatcatac gagttgtctg
      541 tttcttcgag gtcattttga tgtttccaga gcaggttcag ttcaacgcca acaaatggct
      601 tcgtgccaca ggcgatctgg ccgatgttgt tcttaccagt cggattcgtc tggctcgcaa
      661 tcttcaggac gtgccgttca cacgatgggc tctgccagat gaccttgctt cagtgaccgc
      721 ccgttttcga agagtggcaa gcgggacggc agagttggct ggcatggcag tcattgactt
      781 tccacgcctg gatgctctgg agaaggagtt tctctgtgag aggcatctga tcagccgtga
      841 gtttgtcgaa ggcaagaagg aaagcctgct gacattgaac tcagatgaaa cgatcagcat
      901 catggtcaat gaagaggatc acctgagaat acaggcgatt cgttcaggcg tggatctaca
      961 agccgcctgg gaggaactca acgccataga tgacgtgctc gaaaagggga tgcgcttcgc
     1021 tttcgatgag cgatggggtt atttgactgc ctgtcccaca aatatcggca ccggtatgcg
     1081 ctgttctgtg atgcttcatc tgcctggaat ggtcttgacg caacggatcg acaaggtgct
     1141 ctccgccgcg gcacagttga atctcgccgt gcgtggtttt tttggggaag gcagtg
//
LOCUS       DAOQUC010001472         1196 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_170083_length_1196_cov_3.095530, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001472 DAOQUC010000000
VERSION     DAOQUC010001472.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1196
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_170083_length_1196_cov_3.095530"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..549)
                     /locus_tag="PKH07_19220"
     CDS             complement(<1..549)
                     /locus_tag="PKH07_19220"
                     /inference="COORDINATES: protein motif:HMM:NF040244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37130.1"
                     /translation="MKDLLIEALKKSTADYAEIRIELQQRTWLGYRGREMESAGSGSH
                     CGGIVRACFKGGWGLVRFDSLEGLGDQVREACHCARLVGHEKTQLAETIQVDQEKPAK
                     MKRDFRGISLDDKLKLITRYNDIVLKSAPNVRSSYVSYSDSFRTVHFASSRGVYFMEE
                     RPLVACYVSATAADGSLVQRATE"
     gene            complement(687..>1196)
                     /locus_tag="PKH07_19225"
     CDS             complement(687..>1196)
                     /locus_tag="PKH07_19225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37131.1"
                     /translation="VYFLLEKERSRRDTWMYFPEPNLVFGRVCEVRNYSPFAVPKGKT
                     VLCAEVASPPERVSRHDLVARVEDDMVRVGLFQSHEVENILCVDLPYAYPLYELGYQA
                     HLRRVLEFLSPMGQLVTTGRQGLFAYNNLDHSLDIGRRAAEALLRGPDAVRAFYDQRD
                     KLCEYQIVD"
BASE COUNT          292 a          343 c          289 g          272 t
ORIGIN      
        1 ctctgtggca cgctgcacca ggctgccgtc cgctgccgtt gcagagacat aacacgcgac
       61 caacggcctt tcctccatga aatagacacc tcggctggat gcgaagtgaa cggttcgaaa
      121 agagtccgag taagatacgt aggaactcct cacattgggt gccgacttaa gaacgatatc
      181 attgtatcgc gtgatcagtt tcagcttgtc atccagggag atcccgcgaa aatcccgctt
      241 catctttgca ggcttctcct gatccacctg gatcgtttcc gccaattgcg tcttttcatg
      301 accaaccagt cgtgcacaat ggcatgcctc gcgaacctga tctccgagac cttccagaga
      361 atcgaatcga acaagtcccc atcccccttt gaagcaggct cgaacaatcc ccccacaatg
      421 ggaaccggat ccggcagatt ccatctcccg gcctcggtag ccgagccatg tgcgttgctg
      481 caactcgatc cgaatttccg cgtaatcagc agtgctcttt ttcaacgctt cgatgagaag
      541 atctttcatc ccaaatccct ttccgaaaag cgttaggtac cacttcttct agcagattca
      601 agggtgtttt gccaatcccg gcgacactgc gttgcagaga cgagccattg cctttgcctt
      661 gtggcaacaa gaacaactct ctctggtcaa tccacaatct gatattcgca gagtttgtcc
      721 cgttgatcgt agaacgctcg aacggcgtcc ggccctctca gaagtgcttc tgcggcacgc
      781 cgcccaatgt caagagagtg atccaggttg ttgtaagcga aaagcccctg tctgcctgtg
      841 gtgaccagtt gtcccatggg cgacagaaac tctagaactc gtctcaggtg ggcttgatac
      901 cccagttcat agagcgggta agcgtaaggc agatcaacgc acagtatgtt ttcaacctca
      961 tgggactgga aaagccctac acgaaccata tcatcttcga cacgagcgac gagatcgtga
     1021 cgggaaacac gttcgggcgg agaagcaacc tcggcgcaca ggacggtctt acctttggga
     1081 actgcaaaag gagagtaatt cctgacttca cacactctgc cgaaaacaag gttgggttcg
     1141 ggaaaataca tccaggtgtc tctccgtgag cgttccttct ccaggagaaa gtacac
//
LOCUS       DAOQUC010001473         1196 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_170146_length_1196_cov_2.393514, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001473 DAOQUC010000000
VERSION     DAOQUC010001473.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1196
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_170146_length_1196_cov_2.393514"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..520)
                     /locus_tag="PKH07_19230"
     CDS             complement(<1..520)
                     /locus_tag="PKH07_19230"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HNT37132.1"
                     /translation="MLEIGRNDKLLFIGDSITDCGRRNDPEGMGSGYVRMIRDWLLAS
                     QPADAPIVLNKGIGGHKVTDLEARWQTDVLDEKPDILSIKIGVNDVWHAYAATPSGVT
                     PGRYASVFQSILKQVRENLPNCRIVLCEPSIIWPPAPEGANEAMKPYLQSVHVLAQEF
                     NAVCVVPLHKAFT"
     gene            670..>1196
                     /locus_tag="PKH07_19235"
     CDS             670..>1196
                     /locus_tag="PKH07_19235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37133.1"
                     /translation="MTGKKAFCFAVFVGVLWVGMCHAEKIELIKRPEPTEASLGNLLP
                     EDAQEAASSSALESILRRAQNAQTNRSYEEAIQLYQQAEKMPGFEESPQKIPARISMA
                     EALESVGAFEESLKCYQQVLAAIAKSESPSDKRLRNSLLMRMARVYTLTGNQMKSRDV
                     YFQLIQSLEGQERTQA"
BASE COUNT          291 a          299 c          332 g          274 t
ORIGIN      
        1 cggtgaaggc tttgtgcagg ggcacgacac acacggcgtt aaactcctgc gccaacacat
       61 gcacagactg caaatacggt ttcattgcct cattggcacc ttctggagcc ggtggccaga
      121 taatactcgg ctcgcagaga acgatgcgac agtttggaag attctcccga acctgcttca
      181 ggatggactg aaacacagag gcatagcggc ctggggtgac gccacttgga gttgccgcat
      241 aggcatgcca gacatcattc acaccgatct tgatggaaag aatgtccggt ttctcgtcga
      301 gcacatcggt ctgccaacgt gcttcgagat cggtaacctt atgtcccccg attcccttgt
      361 tgagaacaat gggggcatcc gcaggttggc tggcaagcag ccaatcgcga atcatccgaa
      421 cgtagccact acccattcct tccggatcgt ttctgcggcc acagtctgta atgctgtcgc
      481 cgatgaagag gagtttgtcg ttgcgtccaa tctcaagcat cttgtgcaaa agtcctttct
      541 catgaatgat cttggactaa agaaaagcac ttgcggcgaa gaaatgtcaa gcggcgatgc
      601 cggatacttg cctcacgttt caaattggga atgaatgtgt tacgcttcgc tagcgtaaaa
      661 ggtgttgata tgacaggaaa gaaagcattc tgttttgcgg tttttgttgg agtcttgtgg
      721 gtcggcatgt gccatgccga gaagatcgag ttgatcaagc gtccggaacc aaccgaagca
      781 agcctgggga atctccttcc tgaggatgct caagaggcgg cctcttccag tgcgcttgaa
      841 tccatcttgc gcagagccca gaatgcgcag accaatagaa gttacgaaga ggcgattcaa
      901 ctctaccaac aagccgagaa gatgcctggg ttcgaggaga gtccgcagaa gatacccgca
      961 cgcatttcga tggctgaggc cttggagtct gtgggtgcgt tcgaggagtc cctcaagtgc
     1021 tatcagcagg tgctggctgc tattgccaaa agtgagtctc cctccgataa gcgactgaga
     1081 aactcgcttc tgatgcgcat ggcgcgggtt tacactctga cgggaaacca gatgaagtcc
     1141 cgcgatgtct atttccagtt gattcagagt ttggaaggac aggaacgcac acaggc
//
LOCUS       DAOQUC010001474         1195 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_170326_length_1195_cov_3.146491, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001474 DAOQUC010000000
VERSION     DAOQUC010001474.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_170326_length_1195_cov_3.146491"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..681)
                     /locus_tag="PKH07_19240"
     CDS             complement(<1..681)
                     /locus_tag="PKH07_19240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37134.1"
                     /translation="MVLKPACVLIPFLAIVLLQWAPSAFSRDISPLKSCHVWMPMTGV
                     TLEQMNDARRAGYDTMMLKIHPPLAADGKSVDFSIHESRLEQAKAQGFKLVLPILGWV
                     GLGNGLFWDTEEGGGKIMNRLDPFWPEAMEQLEWYFTEVIEHYKQDPAVVAFAPTWGI
                     YGEAGFTSFSAGRSQHALARFNEWRLEQGLSPLNELPTLNDGLNTEYNRFIRFRYLYI
                     EKMFDALIQ"
     gene            complement(707..>1195)
                     /locus_tag="PKH07_19245"
     CDS             complement(707..>1195)
                     /locus_tag="PKH07_19245"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNT37135.1"
                     /translation="AVRDYDTNTKIKPPLRSERDRDAVRQGLKDGTIDAIATDHAPHT
                     IVDKECEYKFATFGTIGFETAFPVIYTHLVEPGIITLNNAVKAMTCTPASVLGINRGT
                     LKVGAQANIMAFDPNLEMTYEQSHIVSKSKNSVFLGQKLKGFPVLTLCCGKVIMSDRK
                     MV"
BASE COUNT          270 a          319 c          331 g          275 t
ORIGIN      
        1 ctgaatcagg gcatcgaaca tcttctcgat gtacagatac cgaaatcgga tgaagcggtt
       61 gtattcggtg ttgagcccgt cattcaacgt cggcagctcg ttcagtggtg atagcccttg
      121 ttccagtcgc cactcattga aacgagcaag agcgtgttgg cttcgtcctg cggaaaaaga
      181 ggtgaatccc gcctcgccgt agatacccca cgtcggcgca aaagccacaa cagcaggatc
      241 ctgcttgtag tgctcaatga cctcggtgaa gtaccactcc agttgttcca tggcctccgg
      301 ccaaaaggga tccaaccggt tcatgatttt cccgccgccc tcttcagtat cccagaaaag
      361 accgttcccc agaccgaccc aacccaggat ggggagcacg agtttgaagc cttgagcctt
      421 ggcttgctcg agtcttgact cgtgtatgct gaaatcaacg gactttccat ctgcagcgag
      481 aggagggtga atcttgagca tcatggtgtc gtagccagca cgacgagcgt cgttcatctg
      541 ttcgagcgtg acgccggtca tcggcatcca gacatggcag cttttcagtg gtgagatatc
      601 gcgagaaaag gcactgggcg cccactgaag caggacgatt gccaagaagg gaatcagaac
      661 gcaagcgggt ttcagcacca tagaggaatt cccgcaaaca gaaacctcag accatctttc
      721 gatcggacat gatgaccttc ccacagcaga gggtgagtac tggaaagccc ttcagcttct
      781 gcccgagaaa gacggagttc ttgctcttgg acacgatatg agactgctca taggtcatct
      841 cgagatttgg gtcaaacgcc atgatattgg cctgggcgcc gactttgagt gtgcctcggt
      901 tgatgcccag aacgcttgcc ggtgtgcagg tcatcgcctt gacggcgttg ttgagagtga
      961 tgatcccggg ttcaaccaga tgcgtataga tgactgggaa cgccgtctcg aatccgatcg
     1021 ttccaaaggt ggcgaacttg tactcacatt ccttgtccac gattgtatgc ggcgcatgat
     1081 cggtggcgat ggcgtcaata gtgccatcct tcagcccttg tcgaaccgca tcccgatctc
     1141 gctctgagcg aagaggcggt ttgatcttgg tgttggtgtc gtagtctctg accgc
//
LOCUS       DAOQUC010001475         1193 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_170817_length_1193_cov_2.741652, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001475 DAOQUC010000000
VERSION     DAOQUC010001475.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_170817_length_1193_cov_2.741652"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..673
                     /locus_tag="PKH07_19250"
     CDS             <1..673
                     /locus_tag="PKH07_19250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37136.1"
                     /translation="GSISRLVPLMESDAVVVTTGAARNTDLDVLAYEAKGIDAAFHLP
                     RCNASEIQSLQAEQVTLIGSDFTESLPTNSLLSERQWQLVDRFLDRDLRFVYVPGVLL
                     PQAFVKWTEQFRDKKTSIEFVVEDPASVLAGINPALVLDAIGAARSTRVRLSCRRPVP
                     LLSLLINPFYPKEKSRGHSYEAAYVDANLLRSRIDSGVNVPVVNVLHDGISELLDAML
                     RVKPS"
     gene            823..>1193
                     /locus_tag="PKH07_19255"
     CDS             823..>1193
                     /locus_tag="PKH07_19255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37137.1"
                     /translation="MMKRLFAAVFVFSVALYSAGSTFCAESPQSAQAVRLSPDLRQLA
                     VSGKSQEVPVFVFLSGKADFKHLVKDAKTCRLAGETIVRGRVSVADLGKLSTLPGVAY
                     VSGSGSRQAPVPPEPEITGKNP"
BASE COUNT          249 a          325 c          311 g          308 t
ORIGIN      
        1 cggttcgatc agccgactgg tgccgttgat ggaatcagat gcggttgtcg tgacgacagg
       61 agccgccaga aacaccgatc tggatgtgct tgcgtatgaa gccaagggca ttgatgcggc
      121 atttcacctc cctcgatgca acgcctcgga gattcagtcg ctccaagcgg aacaggtgac
      181 cttgattgga tcggatttca cggagtccct tccgaccaat tccctccttt cagagcgaca
      241 atggcagctc gtggatcgct ttcttgacag agatcttcgt tttgtctacg tccccggagt
      301 tcttctgcca caggcatttg tgaagtggac ggaacaattc agagacaaga agacttccat
      361 tgagtttgta gtcgaagatc cggcatcggt gctcgctggc atcaaccccg cgcttgtgct
      421 ggatgctatc ggagcagcac gttcgacgag agtgaggctc tcctgtcggc gcccggttcc
      481 tctgttgtcg ctgctcatca acccattcta tcccaaggag aaatcacggg gacattccta
      541 cgaagccgcc tacgtggacg caaacctcct cagatcgcgt atagacagtg gtgtcaatgt
      601 tcctgtcgtc aacgtccttc acgatggcat ctctgaactg ctcgacgcga tgctccgtgt
      661 caagccctct tgaccgttct cgctgcgttt cttttcattg aagtcagcaa caccgctgca
      721 gtctgcattt cggtgctatg ctttcctctt tgcttattga ctgttttgat tccggcatga
      781 taacaatcgc gcagatattc ttgatgagcg aggcatattt ctatgatgaa aagattgttt
      841 gcagccgtgt ttgtgttctc cgtcgccctg tactctgcgg gatcgacctt ctgcgcagaa
      901 tctcctcaga gtgcacaggc agtcagactc agtcccgatc tgcgccaatt ggcagtcagc
      961 gggaaatctc aggaagtccc tgtttttgtc ttcctctccg gaaaggcaga tttcaagcat
     1021 ctggtcaagg atgcaaaaac atgcagactt gctggagaga caatcgtaag aggcagagtc
     1081 agcgttgccg atctgggcaa actctcaacc ctgcctgggg ttgcctacgt ctctggttct
     1141 ggttccaggc aggcaccggt tccgccggag cctgaaatca ctgggaagaa tcc
//
LOCUS       DAOQUC010001476         1193 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_170897_length_1193_cov_2.094903, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001476 DAOQUC010000000
VERSION     DAOQUC010001476.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_170897_length_1193_cov_2.094903"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..587)
                     /locus_tag="PKH07_19260"
     CDS             complement(<1..587)
                     /locus_tag="PKH07_19260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37138.1"
                     /translation="MIKTTIFASVFCSLLLLGRGEPPAHYKNWDLRHGKFFEDNEWVF
                     LKIGKPLWNFADANVVARLTRDLDIIQGKGYNCLEINCYWNYFETDGDGVPDKSLEPL
                     KGFIDSIYDRGMIPCLSVETYGVGGGQIPKGFWTRHPDAVAINNLGMPVTDTEYGTNN
                     PVPSIFSQEYREAARRFIKSIALGVDTKKILYFETT"
     gene            797..>1193
                     /locus_tag="PKH07_19265"
     CDS             797..>1193
                     /locus_tag="PKH07_19265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37139.1"
                     /translation="MVLDQPKRITLRNIALVLALTVHFCVSAQATLSLVPAPKHVSRD
                     TTSPLDVRSQSVAIVLGDKASEPEVIAAETLASNIGKSFSLEWHIRRESESFDARILI
                     HLGQKNTSSEIETFLSTRNIKVNANEDSLD"
BASE COUNT          298 a          313 c          295 g          287 t
ORIGIN      
        1 gtcgtctcga agtagagaat cttcttggtg tccactccaa gcgcaatact cttgatgaaa
       61 cggcgcgctg cttctctgta ttcctgcgag aaaatggaag gaaccgggtt gtttgtgccg
      121 tattccgtat ctgtaacggg catgcccaga ttgttgatgg caacggcatc tgggtggcgc
      181 gtccagaagc ccttcggtat ctgtcccccg ccaactccat aggtttccac agacagacag
      241 ggaatcatgc ctctgtcata gatagagtct atgaatccct tgagcggttc gagagacttg
      301 tcaggaactc catcaccgtc tgtctcaaaa tagttccaat agcagttaat ctccaggcag
      361 ttgtatccct tgccctgaat gatatccaag tctcgtgtga gtcgagcaac gacattggcg
      421 tctgcaaagt tccagagagg ttttccaatc ttcaggaaga cccactcgtt gtcctcgaag
      481 aacttcccgt ggcgcaagtc ccagttcttg tagtgggctg gaggctctcc tctcccaagc
      541 aggagaagag agcaaaacac cgaagcgaag atcgttgtct taatcatttt tcccattcca
      601 atcgcatggt ctggcgaaca ctggatgtga agttaccgga gcaactgctt tctgacaagt
      661 cagaagtccg ttcgaaagag caacagggat ggacaactcc caagacgata gataccatct
      721 cgaaaatgac cttgctggtg gcttgtgagc aacgcttccc agcgggtaag acttgcataa
      781 cgctcaggag gctatcatgg tccttgacca gccaaagaga atcactctcc ggaatatcgc
      841 gcttgtgctt gcgctgaccg ttcatttttg cgtctccgca caagccacgc tgagccttgt
      901 tcctgctcca aagcacgtca gcagggacac gacaagccca ttggatgtac ggtctcagag
      961 tgtggccatt gtcctgggtg acaaggcttc agaacctgaa gtcattgctg ccgagacgct
     1021 tgcttcaaac ataggcaaga gtttctctct tgaatggcac atccgcaggg aatccgaatc
     1081 ctttgacgca cggatactga ttcacctcgg gcagaagaac acaagttctg aaatcgagac
     1141 gtttctcagc acccgtaaca tcaaagtcaa tgccaacgaa gacagcttgg atg
//
LOCUS       DAOQUC010001477         1192 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_171065_length_1192_cov_2.639402, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001477 DAOQUC010000000
VERSION     DAOQUC010001477.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1192
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_171065_length_1192_cov_2.639402"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..444)
                     /locus_tag="PKH07_19270"
     CDS             complement(<1..444)
                     /locus_tag="PKH07_19270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007193276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37140.1"
                     /translation="MRDEKIIVLGARQNSLKGFDLEIPLRKLTVVTGVSGSGKSSLVF
                     DVLYAEGQRRYVETFSAYARQFLDRMDRPLVEEIHHIPAAIAIDQKNPVKTSRSTVGT
                     MTGISDYLRLLFARGAQLFCDGCGRLVRQESPQTIYEDLCEHHRSD"
     gene            complement(453..>1192)
                     /locus_tag="PKH07_19275"
     CDS             complement(453..>1192)
                     /locus_tag="PKH07_19275"
                     /inference="COORDINATES: protein motif:HMM:NF020772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1957 domain-containing protein"
                     /protein_id="HNT37141.1"
                     /translation="WRITSPKADLADKEMYSPQAAQERIPSHADHFVKLVKSTLQKHY
                     EEHGRAGLVCAPFDTELFGHWWWEGPQWLKAVLRGFASTDEVSLVTGSEYLEKSSPSV
                     IVSLPEGSWGKGGFHFIWLNEDTKWTWRHVYEAELEMIRLARKFDATDDPDLQRILKQ
                     MARENLLLQSSDWQFLISTWSARDYAELRFDFHLNAFRKLRDFAERKASGKHLTHADW
                     TAIEELETRDQVFPDIDVSWWKSLDYP"
BASE COUNT          274 a          327 c          312 g          279 t
ORIGIN      
        1 gtccgagcga tggtgttcac aaagatcttc atagatggtc tgtggggatt cctgacgcac
       61 caagcgtcca cagccatcgc agaatagttg tgctcctcgg gcgaagagga gcctcaggta
      121 gtctgagatc cctgtcatcg taccaactgt cgagcgtgac gtcttgacag gattcttctg
      181 atcgatggca attgcagcgg gaatgtgatg gatctcttcc accagaggac gatccatacg
      241 gtcaaggaat tgtcgagcgt atgccgaaaa ggtctccacg tagcgccgct gaccctcggc
      301 gtagagaacg tcgaacacaa gagatgattt gccggatcca ctgactccgg tgacaacggt
      361 cagtttgcga agaggaatct caaggtcgaa gcccttgagg ctattctgcc tggctcccag
      421 aacaattatc ttctcgtcac gcatctcgtt gcttaggggt agtcaaggct cttccaccac
      481 gaaacgtcaa tgtctgggaa tacttgatcg cgggtctcca actcctcaat cgccgtccag
      541 tcggcatggg taaggtgctt tccagaagcc ttcctctcag caaaatcccg aagcttcctg
      601 aaggcgttca gatggaaatc gaagcgcagc tcggcgtagt cgcgagcgct ccaggtgctg
      661 ataaggaact gccagtcgct gctttgtagc aggagattct ctctggccat ttgtttcaga
      721 atccgctgaa gatccgggtc gtctgtggca tcgaatttcc gcgccaaacg gatcatttca
      781 agttccgcct catacacgtg acgccatgtc cacttggtgt cttcgttaag ccagatgaaa
      841 tgaaaaccgc ccttgcccca ggaaccttcc ggcagggaca caatgacgga aggcgatgac
      901 ttttcaagat attcactgcc ggtgaccagt gagacctcat ccgtggaagc aaaccctctc
      961 agaactgcct ttagccactg aggaccttcc caccaccagt gtccaaacaa ctcggtatca
     1021 aagggggcac agaccagtcc agcacggcca tgttcttcgt agtgcttctg aagggtgctc
     1081 ttgaccagtt tcacaaaatg gtcggcatgt gagggaatcc tttcctgtgc ggcctgaggg
     1141 ctgtacatct ctttgtccgc cagatctgcc ttcggagatg tgatccgcca at
//
LOCUS       DAOQUC010001478         1190 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_171574_length_1190_cov_2.828194, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001478 DAOQUC010000000
VERSION     DAOQUC010001478.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1190
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_171574_length_1190_cov_2.828194"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1190)
                     /locus_tag="PKH07_19280"
     CDS             complement(<1..>1190)
                     /locus_tag="PKH07_19280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37142.1"
                     /translation="PSVTPTPKTLAAFVMIQSASGVTLLNRNNIQNLFIEDISPATEF
                     STKKQIRTIELCAAQNGELLQGSHETGLVYLQRGLRWIPDYRIRLDGKGNAFVSLQGT
                     IINELADLENVKMNLVDGVPSFVMQGTLSPMALRDSPPQLGPYFPQLGGSPTNQRDLF
                     SNMMMSQSAGRIQEARFEREGTPESEPPEQGQQEDLFLYQQSNLSLKKGERAQVSLLV
                     VDVTYEDIYTWDIPVTPPMEMWQQIGGDQRRLVEALTAPKAMHQVRLNNTGNEPWTTG
                     VATLFKEGTPLGQQMLLYTSAGNTVDVPVTVATDIQTDKIETEIGRDLNALSLQGYSY
                     ARVRLNGTLTARNFKNRPVRLIVKRKTIGKATSADNDGVIRLSNAPEDQSFGTRASVF
                     SWNW"
BASE COUNT          242 a          309 c          322 g          317 t
ORIGIN      
        1 ccagttccat gagaagacag acgcacgcgt tccgaacgac tgatcctctg gagcgtttga
       61 gagtcggatc acaccgtcat tgtccgccga tgtggctttt ccaatagtct tgcgcttgac
      121 aatcaaacga acgggtctgt tcttgaaatt gcgcgcggtc agagtgccgt tcagacgcac
      181 tcgggcatag ctgtatcctt gcagggacaa tgcgtttaga tcacggccga tctcggtttc
      241 aatcttgtcg gtctgaatgt cggtcgcaac agtgaccgga acgtccactg tgttgccagc
      301 ggacgtgtag agaagcatct gttggccgag cggcgttcct tccttgaaca gagtcgccac
      361 gcctgtcgtc cacggttcgt ttccggtgtt gttgagtcgt acctggtgca ttgccttggg
      421 tgcggtgagt gcttcgacca gtcttctctg gtctcctccg atctgttgcc acatctccat
      481 tggaggagtg accgggatat cccacgtgta gatatcctca taggttacat ccacaaccag
      541 cagactcact tgagcacgct cccctttctt caaggaaaga ttgctttgtt ggtagaggaa
      601 caagtcctcc tgctgacctt gttccggagg ttcggattcg ggagtgcctt ccctctcaaa
      661 gcgtgcctcc tggattctcc cagcggactg tgacatcatc atgttgctga acaggtcgcg
      721 ttgattggtt gggctgcctc ccagttgtgg aaaatagggt ccgagttgag gtggactgtc
      781 tcgaagcgcc atcggggaca gtgtgccctg catgacaaaa ctgggaacac cgtccacgag
      841 gttcattttg acattctcca aatctgccaa ctcgttgatg atcgtccctt ggagcgaaac
      901 aaacgcattc ccctttccgt ctagacggat ccggtagtca ggaatccatc tgagacctct
      961 ctgaagatag accagtcctg tctcatggga gccctgcaaa agttctccat tctgggcagc
     1021 acaaagttct atggttcgaa tctgtttctt tgtgctgaat tcagtcgctg gactgatgtc
     1081 ttcgatgaag agattctgga tgttgttgcg gttcagcaga gtcactcctg atgcagactg
     1141 aatcatcaca aaggctgcaa gtgtcttcgg tgtcggagtg actgagggcg
//
LOCUS       DAOQUC010001479         1190 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_171632_length_1190_cov_2.286344, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001479 DAOQUC010000000
VERSION     DAOQUC010001479.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1190
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_171632_length_1190_cov_2.286344"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="PKH07_19285"
     CDS             complement(<1..312)
                     /locus_tag="PKH07_19285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37143.1"
                     /translation="MSHEYVNGRMHPLEPTSWSWFLRCPVCLDEVTIDQDVHCHRCGR
                     RFPIAHGIPYLSVVDKRWRDHLREIINYFYFYRKIMSRSVEQDRLESDAEHQKLTFQV
                     MA"
     gene            581..>1190
                     /locus_tag="PKH07_19290"
     CDS             581..>1190
                     /locus_tag="PKH07_19290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37144.1"
                     /translation="MSQRVVPERFVQDQFAIGFWVDPPLDNRASERYREIAEANFTTV
                     IGGFGATTPEAVKKQLDLCQKNHLKALVSLPGYRQGAQHGASALEAVRESDKFPDHPA
                     CWGFLTKDEPVASEFADLAFITAHLRKTRPGRLTYINLFPNYANMAQLGAPNYDEHVR
                     RFVAEVTPDVLSMDHYPMMSPHGDGRSGYCENLQSMRDSSLQA"
BASE COUNT          305 a          285 c          321 g          279 t
ORIGIN      
        1 cgccatgacc tgaaaagtca gtttctgatg ctccgcgtcc gattcgagcc gatcctgctc
       61 gacagaacga ctcatgatct tgcgatagaa gtagaaatag ttgatgatct ctctgaggtg
      121 atctctccat cgtttgtcca caacagaaag ataggggatt ccgtgggcaa taggaaagcg
      181 gcgtccacag cggtgacaat gcacgtcctg atcaatagtc acttcatcca gacataccgg
      241 acatcgaaga aaccaactcc aggaagtcgg ctcaagcgga tgcattcgtc cattgacata
      301 ttcatgcgac atcgtgggag tattctaggg acaaaacgca tacagaggca agaacaatca
      361 gccagaagga aagggagtgt tgttgaactc gctgcatagg ggcttagctg tcctgaagtt
      421 ccagagccac aacagtgaga gcggaattcc cgattggaaa ttgctaaaac aaatcgctat
      481 gttgttcggg gagagtcaaa cacatgggag gcagaagaac cgcatgatga agaattccat
      541 tgttctcgta ttctgtgctt ttcttgttgg aggttgtgcc atgagtcaac gtgttgtgcc
      601 ggagagattt gtgcaggatc agtttgcgat cggcttctgg gtggatcctc ctctggataa
      661 tcgcgcctcg gagagataca gagagattgc cgaggcaaac ttcacaacag tcattggcgg
      721 atttggagcc acaacaccgg aggctgtcaa gaagcaactg gacttgtgtc agaaaaatca
      781 cttgaaggca cttgtgtcat tgccaggcta tcgtcaggga gcacagcatg gtgcctcggc
      841 acttgaggct gttcgtgagt cagacaagtt ccctgatcat cccgcctgct ggggttttct
      901 aaccaaggat gaaccggtgg cttctgaatt cgcggacctg gcgtttatca cagctcacct
      961 gcggaaaacg cgacctggca gactgactta catcaatctc ttcccgaact atgcaaacat
     1021 ggcgcagctg ggagcgccca actacgatga gcatgtgcgt cgtttcgtgg ccgaagttac
     1081 tcccgacgtt ctcagtatgg atcactatcc gatgatgtcc cctcatggtg acggccgatc
     1141 tggttactgt gagaatcttc agagcatgcg agacagttcg ctgcaagcag
//
LOCUS       DAOQUC010001480         1188 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_172107_length_1188_cov_2.261253, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001480 DAOQUC010000000
VERSION     DAOQUC010001480.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1188)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1188)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1188
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_172107_length_1188_cov_2.261253"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(645..>1188)
                     /locus_tag="PKH07_19295"
     CDS             complement(645..>1188)
                     /locus_tag="PKH07_19295"
                     /inference="COORDINATES: protein motif:HMM:NF012622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dockerin type I domain-containing protein"
                     /protein_id="HNT37145.1"
                     /translation="AGAVIEFDGRPAGVVESVNGKGAVGYLDALESETVTETPTNTPS
                     TTPSRTPTGTRLPTSTPTHTPSYTRTPTSTPTWTQITVTPTVTPSFTPSRTPTGVPTA
                     TPTKSVKSADFNTDGIVDSKDALLLVEHFMTTETQYDLNSDGRVNARDQFLFSASWQK
                     NPSPCPDPERSELQAGMPVR"
BASE COUNT          259 a          304 c          361 g          264 t
ORIGIN      
        1 gacgttagtg agattgacgt ttggcaggca ttctgcattc atccgactcg tgtgctgaac
       61 aggctcaaca gagtcaaaag agttgccagt agaggctgct attgatgagc agaatggcac
      121 tgagccacag aggacggatc gcgtggcagg tgccggcctt caagtttgcg aagcggttcc
      181 gagtgctgca actgtagccc agatgggata tctggacacg aagttggact ggatcacaga
      241 tcccgcctgc aacctctctg cggacaggat gtccgcgtac ccagggagag atcctcatcc
      301 ggcgaagaac gcagacctct cccagaggga gaggtaaaag ccaggaatgg tgggacctgc
      361 gcttgacagt catccatggg gtctactact gatggacaga aggtgaaggg cttgatcatc
      421 tgtcgtgatc atgatccgaa actgacctat gccttggaaa tgacaaacaa cattgacgtg
      481 cgatactaca atgtgtcgtt caaattgagg gagaatccct aaccagagcg tggagcatga
      541 cttcgggtta gccttaccac ttcactctac ggccaacctg acgtagctca cgctcgccgt
      601 tccccatcga gttccagagg tgggtgagcc agagcaagat tcatctaccg cacgggcatt
      661 cctgcctgca attcactgcg ctcaggatct ggacacggag aggggttttt ctgccacgag
      721 gccgagaaca ggaactgatc ccgggcattg acgcggccat cactgttcag atcgtattgt
      781 gtttcggttg tcataaagtg ttcaaccaga agcagggcat ctttggagtc aacaatgccg
      841 tccgtgttga agtcagcaga tttcacgctt ttggtcggag tggcggttgg cacacctgtc
      901 ggcgtcctgc tcggcgtgaa cgaaggcgtc actgtcggag tcaccgttat ctgtgtccaa
      961 gtgggtgtag acgttggcgt tcgagtgtag gaaggcgtgt gagtcggcgt cgaagtggga
     1021 agacgggttc ccgtcggagt tcgagacggt gtcgtactgg gggtattcgt cggcgtttcg
     1081 gtgacggtct ccgactcgag cgcgtcgagg tatcccacgg cacctttccc gttgacactc
     1141 tccacgactc ccgcgggtcg tccgtcaaac tcgatgaccg cgccggcg
//
LOCUS       DAOQUC010001481         1188 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_172136_length_1188_cov_2.041483, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001481 DAOQUC010000000
VERSION     DAOQUC010001481.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1188)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1188)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1188
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_172136_length_1188_cov_2.041483"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..415
                     /locus_tag="PKH07_19300"
     CDS             <1..415
                     /locus_tag="PKH07_19300"
                     /inference="COORDINATES: protein motif:HMM:NF014841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNT37146.1"
                     /translation="LSSHDEVIRVADILSLHMNLSETTRGLINRARLASMKKGAIIIN
                     CARGGIVDQQALGEALISEAIAGYGCDVLEHEPPEPDNPLIGLENVVVTPHIGSRTYE
                     SVARQAEMATRNLILVLNKKPPLARFQLESKELAK"
     gene            412..>1188
                     /locus_tag="PKH07_19305"
     CDS             412..>1188
                     /locus_tag="PKH07_19305"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNT37147.1"
                     /translation="MSLDRMLERLSQAPAKDVSRQRQISVWKGEPVDRLPILFGVPTP
                     GLEGGIHFDMKECYEDREKMLFETLYSMISQAESPSDSLPGIRANTGTGTLPCCFGLK
                     QEIFPDKMPWLKEHLTKEQIMHLEPDDFDDVTGKGIMPQVLDYQRYFKSQLNGSGWVF
                     LSDTQGPMDIAHLVRGDDIFFDMYDDPPFVHHLMELALAVYISATKAMKANLDEPYDQ
                     GPHVDGVFVANGAVRVCEDTPVILSPHLIERFVVPYTKRAL"
BASE COUNT          287 a          296 c          317 g          288 t
ORIGIN      
        1 cttgtcctct catgatgagg tcattcgagt tgccgatatc ctctcactgc acatgaatct
       61 gtctgagacg actcgaggac ttatcaacag agccagactt gcttccatga aaaagggtgc
      121 catcattatc aactgcgcca gagggggcat tgttgatcaa caggctcttg gagaagcgct
      181 gatatcagaa gccattgcag ggtatggatg cgatgtcctg gagcatgaac caccagaacc
      241 ggacaatcca ctgatcggtt tggagaatgt tgttgtcact ccgcacatcg gttcgcgaac
      301 ctatgaaagt gtcgcaagac aggccgagat ggcaacgcgc aatctcattc ttgtgttgaa
      361 taaaaaacca cctcttgcaa gatttcaact tgagtccaag gagttggcaa aatgagtttg
      421 gataggatgc tcgaaagact ctcacaggct cccgcaaagg acgtctcgcg acagcgccag
      481 atttcggtgt ggaagggtga gcctgttgac cgactgccga tcctgtttgg ggttccgact
      541 cccggactgg aaggcggaat tcattttgac atgaaagagt gctacgaaga ccgggagaag
      601 atgctcttcg agacactgta ttcgatgatc tctcaggcgg agtcaccttc agacagcctt
      661 cccggcattc gtgccaacac cggcacgggc actcttccat gctgtttcgg actgaaacag
      721 gaaatcttcc ccgacaagat gccttggctc aaggagcatc ttaccaaaga acagatcatg
      781 caccttgagc ccgatgattt tgatgatgtg acggggaagg gcatcatgcc acaggtgctt
      841 gactaccagc gttatttcaa gagtcagctg aacggttcag gttgggtctt tctttcagac
      901 acacagggcc cgatggacat tgcccatctg gtcagggggg acgatatctt ttttgacatg
      961 tacgatgatc caccgtttgt ccatcatctg atggaactgg cgcttgcagt ctacatctca
     1021 gccacgaagg ccatgaaagc caatctcgac gagccctatg accaggggcc tcatgtggat
     1081 ggtgttttcg ttgccaatgg tgcggtgcgg gtctgtgaag acacaccggt gattctgtca
     1141 ccacacctga ttgagcgttt tgttgtgccc tataccaagc gggcgctt
//
LOCUS       DAOQUC010001482         1187 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_172234_length_1187_cov_4.453180, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001482 DAOQUC010000000
VERSION     DAOQUC010001482.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1187
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_172234_length_1187_cov_4.453180"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1187)
                     /locus_tag="PKH07_19310"
     CDS             complement(<1..>1187)
                     /locus_tag="PKH07_19310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37148.1"
                     /translation="TVPPQAGAGGGRDAGGADNLFVSMGQPDDTLGFDTTIQFGPDGT
                     TFDPPATLRMQFDPDSMPAGKSPKDMHVYKWIPVGEGGYWQDMGLPSLDYLTNTATVS
                     INSFSSYTLGILLPGGPLAVNTSTLVDNGAGVISTFWATLRNNGYGTLYSVEAQLSEQ
                     HRSEPTPTPTCYTDINTRTSADTVRDFRWSGNNWIPAGKAGAMSKAYRFEIKDSNHGE
                     IPFRLINVSYYGAPPAEDKAAGGTQLLDLSLFTETPLVKEASATIGTAGGILMPAAGG
                     IYSGHQLLVGSGAVNTQTLFEISHPGGEFIPASVRLGPGGIVFTGTVSLTLEYKDTDV
                     PNPPSGDTPENKMALHVWNGASWDLVQGVQTLNTTLNTVQVPLNAFSQEAIYGALPDV
                     VPP"
BASE COUNT          270 a          333 c          323 g          261 t
ORIGIN      
        1 ctggtggaac aacgtcaggc aacgccccgt agatagcctc ttgagagaac gcattcaaag
       61 gaacctgaac ggtattgagc gtggtgttca gagtctggac tccttgcaca agatcccagg
      121 aagcgccatt ccacacatgc aatgccatct tgttctcggg agtatcacca ctcggtgggt
      181 tcggcacatc cgtgtccttg tattccaacg tcaatgagac cgtcccagtg aacacgattc
      241 cgccaggacc caaacgcact gatgccggaa tgaactcacc acccggatgg gagatctcaa
      301 agagcgtttg tgtattgact gctccggaac ccacaagcag ttgatgtcct gagtagattc
      361 cccctgcagc aggcatcagg atgccccccg cagtaccgat agtcgcactt gcttccttga
      421 ccagtggcgt ttcggtgaac agagacaaat ccaacaactg agttccaccc gcggctttgt
      481 cctcagcagg tggggcaccg tagtaagaaa cattgatcag tcgaaatgga atctcaccat
      541 ggttcgaatc cttaatctca aaccgatagg ctttggacat ggctcccgct ttgcccgcag
      601 gaatccagtt gtttcccgac cagcgaaaat ccctgacagt gtcagcagac gttcgcgtgt
      661 tgatatctgt gtagcaggtt ggtgtaggag taggctcgga acggtgctgc tcacttagct
      721 gcgcctcgac cgagtagaga gttccatagc cgttgttgcg aagcgttgcc cagaacgtgg
      781 agatcacacc tgcgccattg tcaaccaggg tgcttgtgtt gaccgccaag ggaccgcctg
      841 gcagcaggat gcccagcgtg taactggaaa aagagttgat ggagacggtg gcagtatttg
      901 tcaggtaatc caaagacggc agtcccatgt cctgccagta gccaccttct ccaaccggaa
      961 tccacttata gacatgcata tctttggggc tctttccggc aggcatacta tcggggtcaa
     1021 actgcatccg cagagtggcc ggtgggtcga aggttgtccc gtcggggccg aattgtattg
     1081 tcgtgtcaaa gccaagtgtg tcgtcgggct gccccatgct gacaaacagg ttatccgccc
     1141 cgcccgcatc tctcccgcca ccggcaccag cctgtggggg aacggtc
//
LOCUS       DAOQUC010001483         1187 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_172248_length_1187_cov_3.521201, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001483 DAOQUC010000000
VERSION     DAOQUC010001483.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1187
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_172248_length_1187_cov_3.521201"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            133..246
                     /gene="rpmJ"
                     /locus_tag="PKH07_19315"
     CDS             133..246
                     /gene="rpmJ"
                     /locus_tag="PKH07_19315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HNT37149.1"
                     /translation="MKVRSSVKAMCEKCKIIRRRGVVRVICKIKKHKQRQG"
     gene            283..654
                     /gene="rpsM"
                     /locus_tag="PKH07_19320"
     CDS             283..654
                     /gene="rpsM"
                     /locus_tag="PKH07_19320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HNT37150.1"
                     /translation="MARIIGVDLPRDKRVEIGLTYVYGIGPAMAIQILETAGVNPDTR
                     VRDLTEEEIGKISTVIQQNYIVEGDLRREINANIKRLMDIGCYRGLRHRRGLPVRGQR
                     TRSNSRTRKGPRRTVGAKKKA"
     gene            750..1148
                     /gene="rpsK"
                     /locus_tag="PKH07_19325"
     CDS             750..1148
                     /gene="rpsK"
                     /locus_tag="PKH07_19325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HNT37151.1"
                     /translation="MAQAARSAGKGKKKVKKDVPRGVAHIQATFNNTIVTITDMQGNV
                     ISWSSAGAMGFKGSRKGTPFAAQMTAEETAKKAMEHGLRQVEVYVNGPGSGREAAIRS
                     LQGIGLSITVIKDVTPIPHNGCRPPQRRRV"
BASE COUNT          320 a          258 c          382 g          227 t
ORIGIN      
        1 cttatgacct gacgcgcggt cgaattacgt accgccagat caaaaagtag actggcagtg
       61 gagactattg tgcctgctat ggcggcacag gagtctcaac tgagtgcgca gtaggaagcg
      121 gagtgttgag aaatgaaagt tcgtagttcc gtgaaggcaa tgtgtgagaa atgcaagatt
      181 atccgcaggc gaggcgttgt ccgcgtgatc tgcaagatca agaagcacaa gcagcgtcaa
      241 ggataaaggg ttcagagcac tggtctaggg aggtgacgga tagtggcgcg tattattgga
      301 gttgacctgc cccgcgacaa gcgagtggag attggtctga cgtacgtcta tggcattggt
      361 cccgcgatgg ccatacagat tcttgagacg gcgggagtta atccggacac tcgagtcaga
      421 gatttgaccg aggaggagat cggaaagata tccacagtga ttcagcagaa ctacattgtg
      481 gaaggtgatc tgcgtcgtga gatcaatgcc aatatcaagc gcttgatgga cattggctgc
      541 taccgaggtt tgagacaccg aaggggtctc ccagtgcgag gacagagaac ccgttccaac
      601 tcgcggacgc ggaaaggacc gcggcgcaca gtgggtgcga agaagaaggc ttagagcaag
      661 acaaacgtct cagcagtggt tcaacctttg cttggtttct gacaggctga acagcgacag
      721 gggacggaac agaaagggaa agcgaagcga tggcacaagc agcccgttcg gcgggtaaag
      781 gtaagaagaa ggttaagaag gatgtaccca gaggggtggc gcacatccag gcgactttca
      841 acaacaccat tgtcaccatt acggatatgc aaggcaacgt gatttcctgg tccagcgctg
      901 gagcgatggg cttcaagggc tccaggaaag gcaccccttt tgccgcgcag atgactgcgg
      961 aagaaacagc caagaaagcc atggaacatg ggctgaggca agtggaagta tacgttaacg
     1021 ggcctgggtc agggagagag gcagcaattc ggtcactgca ggggattggt ttgtccatca
     1081 cggtcatcaa agatgtgacg ccgattccgc acaatggctg tcgtccccct cagagacgcc
     1141 gcgtgtaggg acaacaacgg aggtattact gtgggaaggt atttggg
//
LOCUS       DAOQUC010001484         1187 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_172263_length_1187_cov_3.223498, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001484 DAOQUC010000000
VERSION     DAOQUC010001484.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1187
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_172263_length_1187_cov_3.223498"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..890)
                     /locus_tag="PKH07_19330"
     CDS             complement(<1..890)
                     /locus_tag="PKH07_19330"
                     /inference="COORDINATES: protein motif:HMM:NF021446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2157 domain-containing protein"
                     /protein_id="HNT37152.1"
                     /translation="MSRRNVEWLYGQLPDLVENQIVPEDVAEKIRTYYEPQSQQKPRS
                     VALIICGVLGSLLIGLGIILLLAHNWNQLSRPVRTVICFAPLLLGQALAAWTLLKQRG
                     SIAWQESSSTFLMAAIGASIALIGQTYNIPGDMESFILVWMLLTIPIVYLLGASVPAV
                     FYLIGSVCWAGYARWGAGHAMFYWLLLVPVLPHIYLAYRRSPGGFRSLSLFWVFTITL
                     IIGTGMALEYYGSQQWILAYLSLFSSLYLLSDRVFSRSLFALKNPLFLGGTIGVAVVS
                     LILTYRFPWSHLFATYSYFE"
BASE COUNT          336 a          297 c          316 g          238 t
ORIGIN      
        1 tcttcaaaat aggagtaagt tgcaaacagg tgactccagg gaaaacggta ggtcagaatg
       61 agagatacaa cagcaacgcc gatagttccc cccaggaaca agggattctt cagtgcaaaa
      121 agtgacctgg aaaacactct gtccgacaga agatagagcg atgagaacaa gctgagatag
      181 gcaagaatcc attgctgtga accgtaatat tccagagcca tacccgttcc aatgatcagt
      241 gtgatggtaa aaacccagaa cagcgaaaga ctccgaaaac cccccggact tctacgatag
      301 gcgagataga tgtgcggaag aaccggaacg aggagaagcc agtaaaacat cgcatgtccg
      361 gcaccccagc gcgcgtagcc tgcccagcat accgatccaa tgaggtagaa cacagcgggc
      421 acgctcgcac caagcaggta aacaattgga atcgtcagaa gcatccatac caggatgaaa
      481 ctctccatat cgccggggat gttgtacgtt tgtccgatca gcgcgatgct ggcaccaatg
      541 gccgccatca ggaacgtgct ggaactctct tgccaggcaa tggaaccacg ctgcttcagc
      601 aaagtccagg cggccagtgc ttgacccaga agcaatggcg cgaagcagat gaccgttcga
      661 acgggtcggg agagttgatt ccagttgtgc gccaacagca ggattattcc cagaccgatg
      721 agaagagaac caagcacacc acagatgatc aacgcaacag accttggctt ctgttggctc
      781 tggggttcat agtatgttct tatcttctca gcaacgtctt cggggacgat ctggttctcc
      841 acgagatcag gaagctgccc gtagagccat tccacatttc tgcgactcat aagagacctc
      901 cagtactgag agagtgcggc gttgatctat atgaaacaat gagttcaaca ttctccaccc
      961 ttgatgactg gagcataaga taacacgcca tgcagagagt gtcaaaatgc tcccagatgc
     1021 agggagtttt caatgctgtt gtggagtgtc catctgtggc tccaggacaa atccatcaag
     1081 gggatagttg cgtggctttc gttgggtgag ttccagaatt gttggataca cgtccaccgt
     1141 tcgtgcgcac gggatctgag cgttgggaac cccagggcct gtgagca
//
LOCUS       DAOQUC010001485         1184 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_172996_length_1184_cov_3.123118, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001485 DAOQUC010000000
VERSION     DAOQUC010001485.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1184
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_172996_length_1184_cov_3.123118"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1184)
                     /locus_tag="PKH07_19335"
     CDS             complement(<1..>1184)
                     /locus_tag="PKH07_19335"
                     /inference="COORDINATES: protein motif:HMM:NF020076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT domain-containing protein"
                     /protein_id="HNT37153.1"
                     /translation="DQVLQRCYVVRPARGTRFRDESENQSETVREYRSLYYRLTGDPF
                     NSVPKPKTTRAPSFLFPERDWTEDWDAETVADSEVGGSAVAGRVRGLSLYSSALDVNL
                     SGDEDGTAGPSVAYMEWTLQFQNDSAREREARAQIRLPHNAVCSRLTLWVDGEEREAA
                     FGKRAQVREAYKDVAVVQRRDPALITTTSPDSILLQCFPIPPEQRMKVKIGITAPLEI
                     RLGEALLSLPHFSERNFTILPDFKHSVWIECPSPYETRSQQRVSDNAAVSTNQILLTD
                     QEMQDPSHSVLRLSRPPLGKVFLEGQACLTFRQLPSADKSNAKVCIVLDGSSRMGEVL
                     INWSRILNAFVPGTQFAAVCSGPEVSVWSEGFSTDTSALLAWLSGRTFEGGCDPVPAL
                     GRA"
BASE COUNT          279 a          340 c          306 g          259 t
ORIGIN      
        1 gctctgccga gcgctggaac tggatcacag ccaccctcaa acgtccttcc ggaaagccac
       61 gccaagaggg cacttgtgtc tgtagaaaaa ccctcgctcc aaacggagac ttctggacct
      121 gagcagaccg cggcaaattg tgtcccagga acaaacgcgt tcagtattcg gctccaattg
      181 atcagtacct cacccatacg ggatgaaccg tccagaacaa tacacacttt cgcgttgctc
      241 ttgtccgcgg aaggcagttg acggaaggtg agacaagcct gaccctcaag gaacaccttg
      301 cccaatggag gtcgggacaa tcgaagcacg gaatggctgg gatcttgcat ctcctggtcg
      361 gtcagaagaa tctgattcgt ggacactgca gcattgtcag acactctctg ctgagacctt
      421 gtttcgtacg gcgatgggca ttcgatccag acactgtgct taaaatccgg cagaatggta
      481 aaattacgct ccgaaaaatg gggcagactg agaagcgcct cacccagacg aatttccaac
      541 ggtgccgtga ttccaatctt gaccttcatt ctttgttctg gcggtattgg aaagcattgg
      601 agcaggatac tgtcagggct ggtggtggtg ataagagcgg gatcccggcg ttgcacaact
      661 gcaacatctt tgtaggcttc ccgaacctga gcgcgcttgc cgaaagcggc ctcgcgctct
      721 tcgccatcca cccaaagtgt gaggcgacta cagaccgcat tgtgtggcag acgaatctgt
      781 gcacgggctt ctctctccct tgctgagtca ttctgaaact gcagcgtcca ttccatgtag
      841 gcaacagaag gccccgctgt tccgtcttcg tctcctgaga ggttcacgtc cagtgcactt
      901 gagtacagac tcaaaccccg aacacgccca gcgacagcgc ttccaccaac ttcactatct
      961 gcgactgtct cggcatccca atcctcagtc cagtccctct ctgggaacag gaaagaaggc
     1021 gccctcgtag tctttggctt tggaacgctg ttgaaggggt cccccgtcaa acgatagtag
     1081 agagaccggt attcgcgaac agtttcagac tgattctcgg attcgtcccg aaaccttgtg
     1141 cctcgtgcag gccgcacaac gtaacagcgc tgaaggactt gatc
//
LOCUS       DAOQUC010001486         1183 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_173253_length_1183_cov_2.791667, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001486 DAOQUC010000000
VERSION     DAOQUC010001486.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1183
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_173253_length_1183_cov_2.791667"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..453)
                     /gene="pth"
                     /locus_tag="PKH07_19340"
     CDS             complement(<1..453)
                     /gene="pth"
                     /locus_tag="PKH07_19340"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR00447.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HNT37154.1"
                     /translation="MAGQHVQTEGQKLQAVIGLGNPGRGYSRTRHNVGYWVVDALARK
                     LGSQPKTRSAVSCAVSESYWDEAGPVVLAKPLTYMNESGKAVKGMISHYKLEPDQFTV
                     VYDDLNLPLGTLRLRAKGSAGGHNGIKSIIETLGTDQFQRVRVGLGGPS"
     gene            complement(463..1116)
                     /locus_tag="PKH07_19345"
     CDS             complement(463..1116)
                     /locus_tag="PKH07_19345"
                     /inference="COORDINATES: protein motif:HMM:NF004128.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HNT37155.1"
                     /translation="MQQVDLNVEIRNGIGKGSSHRLRADGRVPGVLYGRGQDNLNLSV
                     SRKELYNLVHGEGAGNTVVKILVAGQDSSQNEQISIVRDVQYDPVTEKIIHVDFQRIS
                     LDEPIHAEIPIHFLGTSIGVKSGGVLEHIIRTLEVECLPLDVPNFVEVDISSLTIGQS
                     IHVRDITAPERVTIKTSQEQTIATVAAPTVVAEPVAEAAPAEGATAEEGKTEEQAKE"
BASE COUNT          240 a          356 c          295 g          292 t
ORIGIN      
        1 actgggtcct cccagcccca cacggactct ctgaaactga tctgttccga gcgtctcaat
       61 aatggacttg attccgttgt gccctcccgc acttcctttg gcacggagcc tcagggtgcc
      121 gagaggcaga ttcaagtcgt cgtaaaccac cgtgaactgg tccggttcca gtttgtaatg
      181 ggagatcatt ccctttacag cctttccgga ctcgttcata tatgtcagag gcttggcgag
      241 aacaacagga ccagcctcat cccagtaact ctccgacacg gcgcaggaca cagccgaacg
      301 agtcttgggt tggctcccca gttttctggc aagagcatca acaacccaat agccaacatt
      361 gtgcctggtt cgggaatagc ccctcccggg gttacccaac ccaatgaccg cttgaagctt
      421 ttgcccctca gtctgcacgt gttgtcctgc cacctttgag ggctattcct tggcttgctc
      481 ttcggtcttg ccctcttcgg cggtcgcacc ttcagcaggc gcggcttcag ccacgggctc
      541 cgcgaccaca gtcggtgcgg cgacagtggc aatcgtctgt tcctgactgg tcttgatggt
      601 cacgcgctcg ggagcagtga tatcccgcac atggatgctc tgcccaatcg tcagactgct
      661 gatatccact tcgacgaagt tcgggacatc caagggcaga cattccacct caagtgtacg
      721 gatgatatgc tcaagcacac cgccggattt gacgccgata ctcgttccga gaaagtggat
      781 cgggatttcc gcatgaatcg gctcatccag cgagatacgc tgaaaatcaa catggatgat
      841 tttctcggtc acagggtcat actggacatc tcgaacgatt gatatctgtt cgttctgaga
      901 actgtcttgt cccgcgacca ggatcttcac gaccgtgttt ccggcacctt ctccgtgaac
      961 cagattatac agttccttcc tggacacact gagattgagg ttgtcctgtc ccctgccata
     1021 gagaacgcca gggacgcggc catcagcgcg caggcgatgc gatgagcctt tgccgatgcc
     1081 gtttcgaatt tccacattca gatcgacttg ctgcattttc ctactctcac tccttgtctg
     1141 ttcggcttca gaatccgact cagatctttc tgggacggcg gca
//
LOCUS       DAOQUC010001487         1181 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_173699_length_1181_cov_3.404973, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001487 DAOQUC010000000
VERSION     DAOQUC010001487.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1181
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_173699_length_1181_cov_3.404973"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(178..858)
                     /locus_tag="PKH07_19350"
     CDS             complement(178..858)
                     /locus_tag="PKH07_19350"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNT37156.1"
                     /translation="MHDSLILIPAFNAEDTLRGVLDSVKPFCENVLVVNDGSIDKTAD
                     VVRSCSWVRLCEHERNKGKGAALATGMTLAKQLGYRTVITMDADGQHCPSEIPGFLQE
                     YAQTGSHIIVGSRFHENSHSEDSVPLVRLISNRLSSALLSWRIGSRITDGQSGFRLYD
                     LSVADLFEGLNPGFVWETQILVRAAQSGLKIAEIPITRRYPDGTKTSHYRSIRDSAQI
                     IKAIFSKH"
     gene            complement(864..>1181)
                     /locus_tag="PKH07_19355"
     CDS             complement(864..>1181)
                     /locus_tag="PKH07_19355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37157.1"
                     /translation="DLRTYHAWVRLKIVELRQTELVISFHSVGQEFVGILAACAFLEY
                     REKSEDKETKDKETRIDGPYRIARESFQFSYQEDPDSIKQRFDNWLRETIVIGLEQWR
                     QQL"
BASE COUNT          279 a          311 c          294 g          297 t
ORIGIN      
        1 acctgcggat cgacccgagc acagccgttg gtgccacagt cgagatagac tatgtcttga
       61 ttccgtagac aacgaggcat gactgctcgt ctccatgtcg gcatgaggtg cggaattcat
      121 ccggtaggag aaacgctgtg ctctcggccg cttccaagcc atatcgcaaa tgctcgatca
      181 gtgttttgag aagattgcct tgatgatctg agccgaatcg cgaatgcttc ggtaatggct
      241 tgtcttggta ccgtccggat agcgtcgtgt gatcggaatc tctgcaatct tcagtccgct
      301 ttgagctgcc cgaaccagaa tctgcgtctc ccagacaaat cctggattca acccttcaaa
      361 gagatctgca acgctcagat cgtacaggcg aaaaccgctt tggccatcgg taatccttga
      421 tccgatacgc caggaaagca gcgctgatga gagtctattt gagatgaggc gaacaagcgg
      481 aacggaatct tcgctgtggg agttctcatg aaaccgtgaa cccacgatga tgtgagaacc
      541 agtctgcgca tactcttgca ggaatcctgg gatctccgag gggcaatgct gtccgtctgc
      601 atccatcgtg atgacggttc tatagcccag ttgtttcgcc agcgtcattc ctgtagcgag
      661 tgcagcacct ttgcccttgt ttcgttcgtg ctcacagagt cgaacccatg aacaggagcg
      721 gacgacatca gcagtcttgt ctatacttcc atcgttgacg acaagaacgt tctcgcaaaa
      781 tggcttgacc gagtccagca caccgcgaag ggtgtcctcg gcattgaagg cggggatgag
      841 aatcaaagaa tcgtgcatct tctttacagc tgttgtctcc actgctccaa tccgatcaca
      901 atagtctctc gaagccagtt atcaaagcgt tgtttgatag agtctggatc ctcttgatac
      961 gagaactgaa acgattcccg tgctatccta tagggtccat cgatccttgt ttctttgtct
     1021 tttgtttctt tgtcttcaga tttctcgcgg tattccaaga aagcgcaagc tgcgagaatc
     1081 cccacaaact cctgtcccac tgaatgaaaa gaaataacca actccgtctg cctgagctcc
     1141 acaatcttca ggcgaaccca ggcgtggtag gtgcgaaggt c
//
LOCUS       DAOQUC010001488         1181 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_173716_length_1181_cov_3.162522, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001488 DAOQUC010000000
VERSION     DAOQUC010001488.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1181
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_173716_length_1181_cov_3.162522"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1172)
                     /locus_tag="PKH07_19360"
     CDS             complement(<1..1172)
                     /locus_tag="PKH07_19360"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNT37158.1"
                     /translation="MLRETLQKEAEQIAQREQLFPPEWHPSDQLPIPGDILPVDQQGL
                     ADRGVFPEKPGIWPSPETDLLPDDQAREFDRKVVEIAQSVGQEKLENRWRRPPSTDRG
                     MEPYGIGLGDGMSGGGKAGDLPAKPLGGRDIAPINIEASGFSPTVPTGVIALDRSEQG
                     VPLSGSRVPVAVSPVGQKDLSRHESIDQFLEAFVETYHDAQGQGYFRVILRPNAKSRA
                     MKVLPKDIVYVLDASGSIGRRVFQEMKTAVIQAIMRLPSTDRFNVLGFQSDVEFLWPG
                     LVEASDVNKALAASFVQKLRPSGMTNIYRSLSSVGDLGRSAERPFLVALFSDGRANIG
                     VTNNREIINDLSTKTGSYTSIFSFGVGSRVNHYLMDLLSYRNKGRFDYAQQEETIER"
BASE COUNT          262 a          335 c          312 g          272 t
ORIGIN      
        1 cgttcgatag tctcctcctg ttgcgcgtag tcgaacctgc ccttgtttcg atacgacagc
       61 aagtccatca gatagtggtt gactcgcgat ccgacgccaa aggaaaagat tgatgtgtag
      121 gaacccgtct ttgtcgaaag atcgttgatg atttccctgt tgttggtgac accgatgttt
      181 gctcttccat cgctgaacag ggcaacgaga aatggcctct cggcacttct tcccaaatct
      241 ccgacgctgg aaagtgaccg atagatgttg gtcatccccg atggacggag cttctgaacg
      301 aaagaggcgg ccaatgcctt attgacatca ctggcttcga cgagtccagg ccagagaaac
      361 tcgacatcac tctggaatcc gagaacattg aaccggtctg tcgagggcag ccgcatgatc
      421 gcctgaatga ctgccgtctt catctcctga aagactcttc ggccaatgct gcccgaagcg
      481 tccagaacat aaacaatgtc cttgggaagc accttcatgg ctcggctctt tgcgttgggt
      541 cgcagaatca ctcggaaata gccctgcccc tgagcgtcgt gatacgtctc gacaaaggct
      601 tccaggaatt gatcgatgga ctcgtggcgc gacaaatctt tctggccaac tggcgaaaca
      661 gcaacaggaa cgcgggaacc tgacaatgga acgccttgct ctgatcgatc cagagcgatc
      721 acaccggtcg gtacggtcgg agaaaagccg gacgcctcaa tgttgatggg tgcgatatct
      781 ctgccaccca atggtttggc gggcagatcg cctgccttgc cgccaccgga cattccatcg
      841 ccgaggccta ttccgtaagg ctccatcccc ctatcggtac tcggtggacg acgccatcgg
      901 ttttccaact tctcctgacc gacgctctgg gcgatttcaa ccaccttgcg atcaaactcc
      961 ctggcttgat cgtcaggcag taagtccgtt tcgggagaag gccagatgcc aggcttctcg
     1021 ggaaacacgc ctcggtcggc aagaccctgc tggtctacgg gcaaaatgtc tccaggaatt
     1081 ggaagttgat ccgaaggatg ccattctggt ggaaagagtt gctcgcgttg cgcaatctgc
     1141 tctgcttcct tttgcagtgt ctcgcgcagc acatcctgac t
//
LOCUS       DAOQUC010001489         1181 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_173819_length_1181_cov_2.184725, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001489 DAOQUC010000000
VERSION     DAOQUC010001489.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1181
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_173819_length_1181_cov_2.184725"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(91..>1181)
                     /locus_tag="PKH07_19365"
     CDS             complement(91..>1181)
                     /locus_tag="PKH07_19365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37159.1"
                     /translation="LKCGVIFWYEDGTPTHHDFPLTELTEDWKSFQFVVEPRERPMAF
                     SVRVESPTWTEDSTALWIDNVSFMAQTEHGTRELLINGGFEEAELVPCRLDGVYLDVI
                     ECYEANLNYRREHWQYAEDPLTFDWGREPALHQLFSHAEYVRHMAQWLRPQNRFVFGN
                     CTPKTPFVTPHLDILGNEVFWKTGEQWTPWSDSQCNYARLMAAQKPYLLLQYGDMSRE
                     EQTRYMKRCLFYGFYPSNQAAPSGGWYWAYPPVVERHRDIFAEYVPLIKNVAEVGWEP
                     LTLASSDNKDVWIERFGSGKNLYLTIYNSASSHQTTLVTLDERLGATPRSRVMNLSTG
                     KRIRWTERGKSFRLTLDSENVTVFNIRR"
BASE COUNT          290 a          326 c          295 g          270 t
ORIGIN      
        1 cagatgcaag cattgccgag ttctgcaatc tcatgtccca cctctttgga tgttcattcg
       61 agatgtatct atcgcagagt ctagatcaca tcagcgacgg atattgaaga ccgtgacgtt
      121 ctctgagtca agtgtcaaac gaaaactctt gccacgctct gtccagcgaa tgcgcttgcc
      181 cgttgacaga ttcatgactc tgcttcgagg cgttgcacca agacgctcat cgagagtcac
      241 cagcgtggtc tgatgtgatg atgcggagtt gtaaatagtc aggtagaggt tcttcccaga
      301 accgaagcgt tcgatccaga catccttatt atccgaactc gccaacgtga gcggttccca
      361 accgacttcg gcgacgttct tgatcagcgg gacatactcc gcgaaaatat cccgatggcg
      421 ttcgaccaca ggcggataag cccaatacca accgccggaa ggcgctgcct gattggaagg
      481 gtaaaaacca tagaacagac atcgtttcat gtagcgcgtc tgttcttcac gactcatgtc
      541 tccatactgg agcagcaaat acggtttctg tgccgccatc agtcgcgcgt aattgcattg
      601 gctgtccgac cacggcgtcc actgctcacc ggtcttccaa aaaacctcgt tgccgagaat
      661 gtcgagatgt ggagtgacaa agggagtctt cggggtacag ttgccaaaca cgaaacgatt
      721 ctgaggacgg agccactgtg ccatgtggcg cacatactct gcgtgagaga acagttgatg
      781 gagcgctggt tcacgacccc agtcaaacgt cagaggatcc tcagcatatt gccagtgctc
      841 gcgacggtaa ttcaggttag cttcatagca ctctatgaca tcgagataga caccatccaa
      901 acgacagggc accaattctg cctcttcaaa gccaccgttg atgagtagtt cgcgtgttcc
      961 atgttccgtc tgtgccataa aagagacatt gtcaatccaa agagcggtcg aatcctccgt
     1021 ccacgtcggg ctttccaccc gaacgctgaa cgccatcggt cgctcccgtg gctccacaac
     1081 aaactgaaac gacttccagt cctcggtcag ttcggtcaga gggaaatcat gatgtgtggg
     1141 cgtgccatcc tcgtaccaga agattacgcc gcatttcagc g
//
LOCUS       DAOQUC010001490         1181 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_173825_length_1181_cov_2.150089, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001490 DAOQUC010000000
VERSION     DAOQUC010001490.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1181
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_173825_length_1181_cov_2.150089"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..847
                     /locus_tag="PKH07_19370"
     CDS             <1..847
                     /locus_tag="PKH07_19370"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNT37160.1"
                     /translation="ATRKSLHAILLVCARGLRNWPSDQISLLATVGRQLSLVCENIEL
                     IRERMQSERLAAVGQAISDISHTIKNLVGGLSASTFLMEDAIRSNEVERYPRLLSVLK
                     DVTNRVGTFMQDLLSFSAGTQELTLASCDILNLLQEVVSIHRDQAHNKKVAIRLEASP
                     DIGYQDLDRKAILSALSNLIVNALDATAQSGSADGFVELKATRDPDETLSISVTDNGT
                     GISPAQLERIWEAFYTTKGSKGTGLGLSLVRRIIEHHGGTISASSEVGKGSVFRISLP
                     RVQSA"
     gene            888..>1181
                     /locus_tag="PKH07_19375"
     CDS             888..>1181
                     /locus_tag="PKH07_19375"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011914846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HNT37161.1"
                     /translation="MKSGVIAIIPARGGSKGVPRKNVRLLGGRPLIAHSIEAALQANC
                     VDRVVVSTDDEEIAQVALNAGAEIPFMRPPELALDHIPVMPVIQELMRKVDPQQ"
BASE COUNT          267 a          320 c          334 g          260 t
ORIGIN      
        1 tgcaacccgc aaatcgctgc atgccatact gcttgtgtgt gcccgaggct tgaggaactg
       61 gccttccgat caaatctcac tgctggcgac cgttggaagg cagttgtccc tggtttgcga
      121 gaacatcgaa ctgatccgcg agagaatgca gtccgagcgt cttgccgcgg tgggacaggc
      181 gatctccgac atcagccaca ccatcaagaa ccttgtcggt ggcctctcgg catccacctt
      241 tctaatggag gatgcgatcc gatcgaatga ggtcgaacga taccctcgcc tgttgtctgt
      301 tctgaaagac gttaccaatc gcgttggcac gttcatgcag gatctgctgt cgttctccgc
      361 aggaacacag gaactcactt tggcctcctg cgacatcctg aatcttcttc aggaagtcgt
      421 cagcattcat cgggatcagg cacacaacaa gaaggttgcg atccgtcttg aggcttctcc
      481 cgacatcgga tatcaagact tggatcggaa ggcaatcctc agtgccttgt caaatctgat
      541 cgtgaatgcg ctggatgcaa cggcgcagag cgggagtgcc gacggctttg ttgagttgaa
      601 ggcaacccga gaccctgacg agacactgag tatctccgtt accgacaatg gaacaggcat
      661 ctcccccgct cagttggagc gaatctggga agccttctac acaacaaagg gttccaaggg
      721 cacggggctc gggctttctc tggtgcgtcg catcatcgag caccatgggg gcacgatcag
      781 tgcctccagc gaagtgggca aaggctcagt cttcagaatc tctctcccga gggtccagag
      841 cgcgtagaga ggtctccgaa cagattacgg aaaggatttc aacagcgatg aaaagtggtg
      901 tcattgcgat tatcccagct cgaggtggct cgaagggcgt cccacggaag aatgtccgcc
      961 tgttgggtgg aagaccgctt attgcccact cgatcgaggc ggcacttcag gccaactgtg
     1021 tggatcgagt tgttgtttca acggatgacg aagagattgc tcaggtggct cttaatgcgg
     1081 gagccgaaat cccgttcatg aggccacctg agttggcctt ggatcacatt cccgtcatgc
     1141 ctgtcatcca ggagctcatg cgaaaagtcg atccgcaaca g
//
LOCUS       DAOQUC010001491         1180 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_173985_length_1180_cov_2.844444, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001491 DAOQUC010000000
VERSION     DAOQUC010001491.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1180
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_173985_length_1180_cov_2.844444"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1180
                     /locus_tag="PKH07_19380"
     CDS             <1..>1180
                     /locus_tag="PKH07_19380"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1925 domain-containing protein"
                     /protein_id="HNT37162.1"
                     /translation="LMTGGYFEPILPMIPDSDKIGQILKLSDYLRERFGVNPIGMWVA
                     ERVWEPHLAKAVNEAGVKYICLDDSHFRMAGLQSSQLYGHYMTEENGCRLAVFPIDMQ
                     MRYMVPFMVPEKTLDYLQSIATPYRERVVVLADDGEKFGVWPGTSKSVYGERWLERFF
                     EMLQANSDWVEMVLFREYLENHPPLGSVYLPTASYTEMMEWALPAKETARIEDIQEEI
                     KTTGREDLERFIKGGFWRNFFVKYPESNNMHKKMLHVHAKIDALSGQERSEPLNKLWA
                     GQCNCSYWHGVFGGLYLNHIRTAVYSNLIEAENLANKAIHGQKDFLDARVEDFYCDGT
                     QSLMCSNRDMNALFHLSRGAALFEWDIRGKCFNVLNTLSRREEAYHRKVLKLASIQKQ
                     T"
BASE COUNT          291 a          273 c          330 g          286 t
ORIGIN      
        1 cttatgactg gcggttactt cgaaccgatc cttccgatga tccccgattc cgataagatt
       61 ggtcagattc tcaagctgtc cgactacctt cgtgagagat ttggtgtcaa ccctattggg
      121 atgtgggttg cggagagggt ttgggagcct cacctcgcca aagcggtcaa cgaggcgggc
      181 gtgaagtata tctgtctcga tgactcacat tttagaatgg ccggccttca gtcatcacag
      241 ctgtatggac attacatgac agaggagaac ggctgcaggc ttgccgtatt tccgattgac
      301 atgcagatgc gctacatggt tccgttcatg gttccggaga agactctgga ttatttgcag
      361 agcattgcga ccccctatcg tgaaagagtt gtggtgcttg cggatgacgg cgagaagttc
      421 ggtgtctggc ctggaaccag caagtcagtc tatggggaac gttggctgga gcgcttcttt
      481 gaaatgcttc aggcaaactc cgattgggtg gagatggttc tattccgtga atacttggag
      541 aatcatccac ccttgggctc tgtctatctt ccaactgcat cctacacgga gatgatggaa
      601 tgggctcttc ctgcgaaaga gactgcgaga attgaggata ttcaagagga gatcaagacc
      661 accggacgag aggacttgga acgattcatc aaaggtggct tctggagaaa tttctttgtg
      721 aagtatcccg agagcaacaa tatgcacaag aagatgctcc atgttcacgc gaagattgac
      781 gcgctctccg gacaggaacg atctgagcca ctcaacaagt tgtgggcggg gcaatgcaat
      841 tgctcgtatt ggcacggggt gttcggtggc ctctacctca accacattag aaccgcagtc
      901 tacagcaatc tcattgaggc cgagaatctc gcaaacaaag caatccacgg tcagaaggat
      961 ttcctggacg cacgtgtgga agacttctac tgtgacggca cgcagtctct gatgtgttca
     1021 aacagggata tgaacgctct gtttcatctg agtcgtggtg ctgcgttgtt cgagtgggac
     1081 attcggggaa aatgcttcaa tgtactcaac acactaagcc gtcgagagga agcctaccac
     1141 aggaaggtgc tgaaactcgc ctcgattcag aagcagacgg
//
LOCUS       DAOQUC010001492         1179 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_174235_length_1179_cov_2.906584, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001492 DAOQUC010000000
VERSION     DAOQUC010001492.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1179
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_174235_length_1179_cov_2.906584"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            71..>1179
                     /locus_tag="PKH07_19385"
     CDS             71..>1179
                     /locus_tag="PKH07_19385"
                     /inference="COORDINATES: protein motif:HMM:NF002674.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tm-1-like ATP-binding domain-containing protein"
                     /protein_id="HNT37163.1"
                     /translation="MKQICLIGAFDTKGAEYAFVREQILARGHRVITVNTGVMGTTDL
                     FTVDIEADEIAKAGGGELLKIRENKDRGEAMKVMCSGAPVVLRSLHDQGRIDGVLGMG
                     GTGGTTVVSAAMRALPLGLPKVCVSTTASGDVSMYVGTTDITMIPSIVDVAGINRISR
                     IIFARAAGAVCGMVEAQIAKTEADKPVITASMFGNTTTCVNACMGKLAEKGYEVLVFH
                     ATGTGGKTMESLVWEGLVDAVLDITTTEWADTLCGGVFDAGTQRLDAPGQMGVPHLIV
                     PGCVDMANFGPMSTVPEAYRKAKRVFYEWNPSVTLMRTNKEENAKMGTIFAEKANAAV
                     GPVAFLIPLKGVSILDGEGQPFCDREADQAMFDAI"
BASE COUNT          279 a          270 c          367 g          263 t
ORIGIN      
        1 aaagtttggc gggagaatca ggtgggtgct tttccaggcg gcagctcatg caagtaagga
       61 aggagtcgag atgaaacaaa tatgcctcat tggagcattt gacaccaaag gcgctgagta
      121 cgcttttgtg cgcgaacaga tccttgcgag aggtcatcga gtgatcaccg tcaacaccgg
      181 agtgatggga acgaccgatc tcttcaccgt tgacatcgaa gcggacgaga ttgccaaagc
      241 cggtggtggg gagcttctca aaatacgtga gaacaaggat cgcggcgaag caatgaaggt
      301 catgtgttcc ggtgcgcctg tcgttctcag atcactccat gaccaaggcc gaattgacgg
      361 ggtcctcggc atgggtggaa cggggggaac cacggttgtc agcgccgcca tgagagcatt
      421 gccattgggg ctgccgaaag tctgtgtgtc cacaactgcc tcgggcgatg tgtccatgta
      481 tgtcggaacg acagacatca cgatgatccc ttccattgtg gatgttgccg gcatcaaccg
      541 catttctcga atcatctttg cgcgggctgc gggtgcggtg tgtggcatgg tggaagcgca
      601 gattgccaag acggaagcgg acaagccggt gatcacggcg tccatgtttg gtaacacgac
      661 cacctgtgtc aatgcgtgta tgggaaaact tgctgaaaag ggatatgagg ttctggtttt
      721 ccatgcgact ggaacgggtg gaaagaccat ggaaagcctt gtctgggaag gacttgttga
      781 tgcggttctg gacatcacca ctaccgaatg ggcggataca ctgtgtggcg gagtttttga
      841 tgcgggcact cagcgtcttg acgcacctgg acagatgggc gttcctcatc tgatcgttcc
      901 tgggtgtgtg gatatggcga attttggccc gatgagcacg gttccggaag cctatcgaaa
      961 ggccaagcgt gtcttctacg aatggaatcc atccgtcacg ctgatgagaa cgaataagga
     1021 agaaaatgcg aagatgggaa cgatttttgc agaaaaggca aacgctgcag tggggcctgt
     1081 tgccttcttg attcctctca aaggcgtctc gatcctggat ggagagggac aacctttctg
     1141 tgaccgcgaa gccgatcagg caatgttcga cgccatcaa
//
LOCUS       DAOQUC010001493         1179 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_174269_length_1179_cov_2.545374, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001493 DAOQUC010000000
VERSION     DAOQUC010001493.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1179
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_174269_length_1179_cov_2.545374"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            224..>1179
                     /locus_tag="PKH07_19390"
     CDS             224..>1179
                     /locus_tag="PKH07_19390"
                     /inference="COORDINATES: protein motif:HMM:NF012965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydrofolate dehydrogenase/cyclohydrolase
                     catalytic domain-containing protein"
                     /protein_id="HNT37164.1"
                     /translation="MRKGFLGSTFVCFVALIVLVPCLPAPLLADEEPEDVLTQTKVSG
                     QRTWRVPSPLFQELLSENAPQLPGEAGFLWTHPFMADRMRSIGVQFGVEYSLDETGQL
                     MADLNFHPIQESVNMGKVFAEKRYPQAKPLVKLNWYRARGEGMPTVSSDGQTMLFAPK
                     NRQKYLEETEELLKQYGNDIWGVFAGDEMVLKQRKAIFAILESGVEKHPYIKDIEADI
                     RQRFGLGTYGIPSGQDDPEPYKWLAMSLWLDEQVCLLVADLRKIIDRVAPHVVLVGDD
                     PASHAYVRSTVKKCAQHGIAARRSDLPANTSTDTLLALLGELA"
BASE COUNT          251 a          282 c          356 g          290 t
ORIGIN      
        1 cggcctgcgc ctgcgggtgc gtgggctatt tccccacccc cttggacacc tgctcagtca
       61 aacatggaaa acacctgttg actatcatta gtgaacatgg atacattaga gtgaacatgt
      121 tcgctttact ttccgcgaac cgtgcacctc tctttgcaga ggggggcatg agattctgtg
      181 agagttgtat gtcgaacgta atacgaggat aggagatatt gagatgagaa aagggttcct
      241 tgggtctacc ttcgtgtgct ttgtggcttt gatcgttctt gtgccgtgtc tgcccgctcc
      301 tttgctggct gatgaggagc cagaagatgt gctcacccag accaaggttt caggtcaacg
      361 aacttggcgg gtcccaagtc ctctctttca ggagttgctc tcggagaatg ctcctcagct
      421 tcctggggaa gcaggttttc tctggacgca ccctttcatg gcggatcgga tgcgttccat
      481 cggtgttcag ttcggtgttg agtactcact ggacgagaca gggcagttga tggcggatct
      541 gaactttcat cccattcagg aatccgtgaa catggggaag gtctttgcag agaagaggta
      601 tccacaggcc aaaccgcttg tgaagttgaa ttggtaccga gcccgcggag aggggatgcc
      661 gactgtcagc agcgacggtc agacaatgct gttcgcgccc aagaatcgtc agaagtatct
      721 ggaggagacc gaggaacttc tcaagcagta cggcaacgac atctggggtg tttttgccgg
      781 tgatgagatg gttctgaagc agagaaaggc gatctttgcg attctggaaa gtggtgtaga
      841 gaagcatccc tatatcaagg atattgaggc ggacattcgt cagcgttttg gccttggcac
      901 gtatggcatt ccgtcgggac aagacgatcc agagccgtac aaatggcttg ccatgagcct
      961 ttggctggat gagcaggtct gtcttctggt tgccgacttg cggaagatta ttgaccgtgt
     1021 tgcgccacat gtcgtgctgg tgggtgacga tccggcttcg catgcctatg ttcgatcgac
     1081 ggtgaagaag tgcgcgcagc acggcattgc cgcgcggcga agcgacctgc ccgcgaacac
     1141 ctcgactgac acactgctgg cacttctggg ggaactggc
//
LOCUS       DAOQUC010001494         1179 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_174318_length_1179_cov_2.274021, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001494 DAOQUC010000000
VERSION     DAOQUC010001494.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1179
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_174318_length_1179_cov_2.274021"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="PKH07_19395"
     CDS             <1..223
                     /locus_tag="PKH07_19395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37165.1"
                     /translation="PGEAPRVHGPMRLAKLAHFLLREIFPAHDTLCKTEGDLMMAFYQ
                     GDKTAASQLAQLQGQTKDYMDRVWGSEEK"
     gene            220..1131
                     /locus_tag="PKH07_19400"
     CDS             220..1131
                     /locus_tag="PKH07_19400"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNT37166.1"
                     /translation="MTPTAIVLLLISAASHATWNLLSKRRSSTASFFLLANLFGALFL
                     TPVLWVRWEIPLTMPTSVWFLLAVTGFFQALYYISLARAYCSGDMSIAYPIARSLPVI
                     LVLIVTLLIGQASQVSLLCFAGVILIVAGCFVLPMKHYRQFKIKNYWNPTCLWALSAA
                     VGTTGYSMVDDRALRILKASLEQSEGTQSLPVIVYALFEAISSSFWLSLFVLVPKQTR
                     KPTLQVFRTLKGQTALTGLCIYATYVLVLISMGYVKNVSYVVAFRQLSIPLGAIMGIV
                     LLREDRPLPKIVGVLTMFAGLILVGFS"
BASE COUNT          244 a          321 c          298 g          316 t
ORIGIN      
        1 tcctggagaa gcgcctcgag tgcatggccc catgcggctg gcaaaacttg ctcattttct
       61 tcttcgcgag atattccccg cacacgatac actctgcaag acggaaggcg atctgatgat
      121 ggctttctac caaggggaca agacggccgc ctcacagttg gcgcaacttc aaggccagac
      181 gaaagactat atggatcgtg tatggggatc tgaagagaaa tgaccccaac cgcgatagtt
      241 cttctgctga tctctgcggc aagtcatgca acgtggaatc tcctgagcaa gagacgtagt
      301 tcaacagcct cattctttct tctggcaaac ctcttcggag ctctcttcct gacacctgtt
      361 ctttgggtaa gatgggagat tcctttgaca atgccgacat ctgtctggtt cttgctggca
      421 gtgactggtt tttttcaggc actctactac atctctctcg ccagggcgta ctgctcgggt
      481 gatatgtcca tcgcctatcc catcgcccgt tcattgccag tgattctggt cttgatcgtt
      541 acgcttctca tcgggcaagc cagtcaggtg agtcttctct gcttcgctgg tgttatcctg
      601 attgtcgcag gatgtttcgt gcttccgatg aagcactacc gccaattcaa gatcaagaac
      661 tattggaacc cgacctgcct ttgggctttg tcggcagccg tgggaaccac agggtactcg
      721 atggtggacg acagagcctt acgaattctg aaagcatcgc tggagcaatc ggaaggcact
      781 cagagccttc cagttatcgt ctacgcactg tttgaagcca tcagttcctc gttctggctg
      841 tctctgttcg ttctcgtgcc caaacaaacc agaaagccaa cgctccaggt atttcgaacc
      901 ctcaaagggc agacagcgct cacgggactg tgcatctatg ccacttacgt gcttgtgctc
      961 atttccatgg gctatgtgaa gaacgtcagc tacgtcgtcg cctttcgtca gctcagcatt
     1021 cctctgggcg cgattatggg cattgtttta ttgcgggaag accgtccgct tcctaagatt
     1081 gtaggggttc tgaccatgtt tgcagggcta atcctggttg gcttcagcta ggttcattta
     1141 ctgcccggct tcgggagcgc gcagagcctc tacaaccga
//
LOCUS       DAOQUC010001495         1177 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_174673_length_1177_cov_3.553476, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001495 DAOQUC010000000
VERSION     DAOQUC010001495.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1177
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_174673_length_1177_cov_3.553476"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            228..>1177
                     /locus_tag="PKH07_19405"
     CDS             228..>1177
                     /locus_tag="PKH07_19405"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HNT37167.1"
                     /translation="MNQLRLFEEESTNLISVREAATWASEYLQRQITISNISYLIQYG
                     RIHKYGGSGNPLVDVSELRKYYDSDDRQLRWKSQLGEDLNWRLSFVEYKESERTKHVH
                     RLHPYKGKFIPQLVEYFLDSHTDEFKKNQYFRAGDIVLDPFCGSGTTLVQANELGIHA
                     IGIDVSAFNALISNVKVARYDLQRVAEAMQYMTGRLREFQRMRSNSAFEEHLLAELAT
                     FNSRYFPAHVYRKKVRQGEIDEDQYGMAREKEFLPVYWELVKRYGLQISQTNGDSFLG
                     KWFLYPVREEIECLFEALKGIEDKNVKKILAVILSRTVRS"
BASE COUNT          319 a          267 c          315 g          276 t
ORIGIN      
        1 gttccgcctt cgatcgcggt cggttggttg ttgatggaca actacatcat cacagggaat
       61 caactgtcca tcaacaacca accgaccgcg atcgaaggcg gaacacattc gattgtgaag
      121 gacaatctct aggaaagacg ctgggtgctg gcttgacttc agccgcgcgt cggggtagaa
      181 ggagacttgc ttggagtcgc ggagacgacc taggtttggg agcacgcatg aatcagctta
      241 ggctatttga agaagagagc acgaatctga tcagcgtccg tgaagcagcc acttgggctt
      301 ccgagtacct gcagagacag atcacgatta gcaacatatc ctatttgatt cagtacggaa
      361 gaatccacaa gtatggcggt tccggaaatc ctcttgttga tgtttcggaa ctccgcaaat
      421 actacgactc agatgacagg cagttgcggt ggaagagcca gcttggtgag gatctgaact
      481 ggcgcttatc ttttgtggag tataaagaat cagagagaac aaagcacgtt cataggctgc
      541 atccctataa aggcaaattc atccctcaac tggtggagta tttcctcgat tcccacacag
      601 atgaattcaa gaagaaccag tacttccgtg ctggcgacat tgtcttggat cctttttgtg
      661 gcagcgggac aactttagtt caggcaaatg agctgggaat ccacgcgata ggcattgatg
      721 tatcggcttt caacgcactc atcagcaatg taaaagttgc gagatatgac ctccaacgcg
      781 ttgctgaggc catgcaatac atgacgggca ggctgaggga attccagcga atgcgttcca
      841 acagcgcttt tgaagagcat ctgcttgccg aactggccac gttcaactca agatacttcc
      901 cagcccatgt gtacagaaaa aaggtcagac aaggtgagat tgatgaggac cagtatggca
      961 tggcacgcga gaaagagttc cttcctgtgt actgggaact tgtgaagcgg tatggtctac
     1021 agatctctca gaccaatggg gattcctttt tgggcaagtg gttcctgtac ccggtgcgag
     1081 aagagataga gtgtcttttc gaagcgctca aaggcatcga ggataagaat gtcaaaaaaa
     1141 tactggccgt tatcctaagc agaaccgttc gctcctg
//
LOCUS       DAOQUC010001496         1177 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_174680_length_1177_cov_3.447415, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001496 DAOQUC010000000
VERSION     DAOQUC010001496.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1177
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_174680_length_1177_cov_3.447415"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1177)
                     /locus_tag="PKH07_19410"
     CDS             complement(<1..>1177)
                     /locus_tag="PKH07_19410"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNT37168.1"
                     /translation="LVQSGFLSKEFAVEALDAMVTRMPYNEAAKVLSEKQFDALFFLT
                     GAVSWRKDFAFIQDVIQDRNVCIIATGDFLLTQGQKVMSEFPFLDAVLLDLTSSAVVE
                     YLRGDLTGQPRQNLHYRHEGNLILGERKLESKTFTLPLPRYDLFPYKRYRIPHGKGKP
                     FASILTDYGCPFRCSFCIGGKLGYKTREISNVMDELRYVHGLGIRELWVKDLTFGCHR
                     RHSIEFCQALIDSGMRFDWVTLSRVDVMDEEILSLMARAGCHTIQLGVESANETILES
                     ISKGISKDRILQVFRTCRRLGIRTLAHFIIGLPGETEQSALETIAFAKQLDPDFASFN
                     VATPRIGTDLRESAIAKGYTSEDLDVLDNSLSMPVMETETLTSSKLWELRNRAIREFH
                     "
BASE COUNT          291 a          324 c          294 g          268 t
ORIGIN      
        1 gtgaaactcc cgaatcgcgc ggttcctgag ttcccagagt ttgctgctgg tcaacgtctc
       61 ggtttccatc acgggcatgg aaagcgagtt gtccagcaca tccaggtctt cgctcgtgta
      121 gcctttggca atggcactct cccgaaggtc ggttccaatg cggggggtgg caacattgaa
      181 cgaggcaaaa tccggatcca actgcttcgc gaacgcgatg gtctccaaag cactctgctc
      241 cgtttcaccc ggcagaccga tgatgaagtg cgccagcgtc cgaatgccta accttctgca
      301 cgtcctgaat acttgaagga tccggtcttt cgagatgcct tttgagatgg attcgaggat
      361 tgtctcattg gcgctctcaa cacccagttg aatcgtatga caacctgcgc gcgccatcag
      421 ggaaagaatc tcttcatcca tgacatccac acgtgacaat gtaacccaat cgaaacgcat
      481 cccggaatca atgagcgcct gacagaattc aatgctgtgc cttcgatggc atccgaacgt
      541 caagtccttg acccagagtt cgcggatacc caaaccgtgg acgtaccgaa gctcatccat
      601 gacgttcgag atttcccgtg tcttgtagcc cagtttcccg ccgatgcaaa acgaacaccg
      661 aaacggacag ccataatcag tcagaatgga agcgaacggc ttccctttcc cgtggggaat
      721 tcgatagcgt ttgtatggaa agagatcgta gcgcggaaga gggagcgtga atgttttgga
      781 ctcaagtttt ctctcgccca atatcaagtt cccctcgtgc cggtagtgaa ggttctgcct
      841 cggctgaccg gtcaagtcac ctcggagata ctcgacgact gccgaagacg ttaagtccaa
      901 gagtacggca tccaggaacg ggaactcgct catcaccttc tgaccctgtg tcagcaaaaa
      961 gtcgccagtg gcgatgatgc agacatttcg atcttggatg acatcctgaa tgaatgcaaa
     1021 atcctttcgc caggagacgg ctcccgtgag aaaaaacagc gcatcgaatt gtttctctga
     1081 aagaaccttt gctgcctcat tgtaaggcat tcgggtaacc atggcatcga gcgcttcaac
     1141 cgcaaattct tttgagagaa aaccactctg caccagc
//
LOCUS       DAOQUC010001497         1177 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_174711_length_1177_cov_2.841355, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001497 DAOQUC010000000
VERSION     DAOQUC010001497.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1177
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_174711_length_1177_cov_2.841355"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..397)
                     /locus_tag="PKH07_19415"
     CDS             complement(<1..397)
                     /locus_tag="PKH07_19415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37169.1"
                     /translation="MRSPIIWRLVTGTVVLAAVVLVSFVSPAFAGGDEEEFLIQHNER
                     KHFWFPQDARSLGMGGTFVAVPGDTMSVYGNPAGLAAIENLQLRASYFWDKVSGNLPT
                     GEPSPPVRPSVEERLHGPSFLVAVPNPFFP"
     gene            complement(454..>1177)
                     /locus_tag="PKH07_19420"
     CDS             complement(454..>1177)
                     /locus_tag="PKH07_19420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37170.1"
                     /translation="YGEVRRFTLPGRPEIPDLGQKLLQSNLARDIAVVAPGGCATSNG
                     AYILIGTGLVQTLGPITNYGGTMFGSYWWPLDLGVDLEVTKTQCGYYMLDAMGRVYVF
                     GDALWHGHVEFDYDLARDLELDPLGNGYVILDGFGALHPYGEAPFVDRINEIVAGVAA
                     AGKLDLYFFGWDIARAFELESNASGQVTGFLVLDGYGVVRRHELVAPMLSNSSIITSV
                     TYPIDIARDLETGNQTGTLPSE"
BASE COUNT          306 a          345 c          281 g          245 t
ORIGIN      
        1 ttgggaagaa agggtttggc actgcaacga gaaaagaggg accatgcagg cgttcttcga
       61 ctgagggccg aactggaggt gaaggctctc ccgtaggaag attcccactc accttatccc
      121 agaaataact cgcccggagc tggagattct cgatggcagc aagacccgct gggttgccat
      181 agacgctcat cgtgtccccg ggaacagcaa caaaagtccc gcccattccc aagctcctcg
      241 catcttgtgg gaaccagaaa tgcttccgtt cgttgtgctg aatcagaaac tcttcttcgt
      301 caccacctgc aaatgcaggt gagacaaaag aaaccagcac aacagcggca agaaccactg
      361 taccggttac cagtcgccag atgatcggac ttctcatgtc gatcctccaa tctcactcgt
      421 tatcgaatcg tctctactcc aaaagcctca gcgtcactcg ctgggcaaag taccagtttg
      481 attgcccgtt tccaagtctc gcgctatgtc gatcggatat gtcacactcg tgataattga
      541 cgagtttgac aacatgggcg ccaccaattc gtggcgtcga acaacaccat atccgtcaag
      601 aacaaggaat ccagtaacct gaccactcgc gttcgactcc agttcaaagg ctcgggcgat
      661 atcccacccg aagaaataca gatcgagttt ccctgccgca gcaacacctg caacaatctc
      721 gttgatgcga tcgacgaagg gtgcctcgcc gtagggatga agtgctccga agccgtcaag
      781 gatcacgtag ccgttcccca atggatcaag ttcaaggtct cgcgctaagt catagtcaaa
      841 ttcgacatgg ccgtgccaca gtgcgtcgcc aaagacgtac actcgaccca tcgcatccag
      901 catatagtaa ccacattgag tcttcgtcac ttcgagatca actcccagat ccagtggcca
      961 ccaataggag ccaaacatcg ttccaccgta gttggtgatt gggccgagag tctgaacaag
     1021 acctgttcct ataagaatat aggcaccgtt gctcgtagcg cacccaccag gagccacaac
     1081 tgcgatgtct ctggcaaggt tagactgaag cagtttctgt cccaagtcag gaatctccgg
     1141 ccgtcctggc agggtgaacc gccgcacttc cccatag
//
LOCUS       DAOQUC010001498         1176 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_174955_length_1176_cov_2.859054, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001498 DAOQUC010000000
VERSION     DAOQUC010001498.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1176
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_174955_length_1176_cov_2.859054"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            340..>1176
                     /locus_tag="PKH07_19425"
     CDS             340..>1176
                     /locus_tag="PKH07_19425"
                     /inference="COORDINATES: protein motif:HMM:NF015891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HNT37171.1"
                     /translation="MSVLRACLCLTVLLMLILAMPVWAQEEAAAEEVAAEEVAMQPAD
                     ESGSTEEIEAKLDQMVGPLTIAGVKWRDFLGVLSRTTGITFIPSPKVTANVDINFPQK
                     VSLRSILDEFLPLHGLVYRVTPTAIRVMTKEEEPLPEVELQERIFTVLYTTVEKITNA
                     IQTLKTPNGQIVSDPATKQIIVRDTPEALAEMEALVMKLDVQTQMRIFDIKYVDTATV
                     VTQIRDLLSGEKGSIQEDPKTGKIIITDIPDVLERVAQIIAEIDMKTTLTFFKLNFAD
                     PKQ"
BASE COUNT          297 a          294 c          318 g          267 t
ORIGIN      
        1 gggtttggac ggccagaccc agagcaagga gtagtgctga gcaggtcagt gcgtgcaaac
       61 atgtggctat gctcactcct gtgcaatgct tgctcgctga ggaaaccgcc tcttctggag
      121 tccggctccc tgtgagcggt acctgctgaa gcataggcgc tgtccgattt cgcggactga
      181 atctcaggga tagttcgacc cgatcatcgg gtttgggctc ccatgaggac tgaaaatgca
      241 tcttgtaaaa gggcggtaag tatggtatcc actagacaat gtgtttatga ccaaaagcaa
      301 ccagatatct gggggactaa tatgagccga ggagttgcca tgagtgttct aagggcttgt
      361 ctctgtctca ctgtccttct gatgctcatt ctcgccatgc cagtctgggc gcaggaagaa
      421 gcggctgcag aagaagtggc tgcagaagaa gtggcgatgc aacctgcgga tgagtctggg
      481 agcacggaag agattgaagc caaattggat caaatggttg gccctttgac aattgcgggt
      541 gtcaagtggc gcgacttcct cggtgtgctc tcccgaacga cgggcattac gtttattcca
      601 tcgcctaaag ttaccgccaa tgttgacatc aatttcccac agaaggtcag cttgcgttcc
      661 attctggatg agtttctgcc cttgcacggt ctggtctatc gtgtgacccc aaccgcaatt
      721 cgtgtcatga ccaaagaaga ggaacctttg cctgaagtgg aactgcagga gcggattttc
      781 acagttctct acacgaccgt cgagaagata accaatgcca ttcagactct gaagacgccc
      841 aacgggcaga tcgtctcgga tccggccacc aagcagatca tcgtccgaga cacacccgaa
      901 gcacttgcgg aaatggaagc gctggtgatg aaactcgacg tgcagacaca gatgcgaatt
      961 ttcgatatca aatatgttga cacggcaacg gttgtcactc aaatccgcga tctactcagt
     1021 ggcgaaaagg gctccattca ggaagatccc aagacgggaa agattatcat cacggatatt
     1081 ccagacgtgc tcgaacgtgt ggctcagatt atcgccgaga tcgatatgaa gaccacgctg
     1141 actttcttca aactcaactt cgcggacccc aaacag
//
LOCUS       DAOQUC010001499         1176 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_175007_length_1176_cov_2.423729, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001499 DAOQUC010000000
VERSION     DAOQUC010001499.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1176
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_175007_length_1176_cov_2.423729"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..620)
                     /locus_tag="PKH07_19430"
     CDS             complement(<1..620)
                     /locus_tag="PKH07_19430"
                     /inference="COORDINATES: protein motif:HMM:NF024909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HNT37172.1"
                     /translation="MPKRFLDPDLIRALDCVAAVVRKIHLGEMQGGHWSHRPGFGLDF
                     KDHRPYVQGDDPRRVDWNLYVRLGRLFVKVFHDEEDVDIHLVLDSSASMRCGKPTKHY
                     YSSQVAASLAYIGLTSQERVGLWTFNNGTCVGIPPSRGNDHFCRIFDQLASLEPEGTT
                     GMKEALAELALRTPRGGVFILLSDFVDCPHWEYSLKMITNRPRSEL"
     gene            695..>1176
                     /locus_tag="PKH07_19435"
     CDS             695..>1176
                     /locus_tag="PKH07_19435"
                     /inference="COORDINATES: protein motif:HMM:NF025841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 domain-containing protein"
                     /protein_id="HNT37173.1"
                     /translation="MSQLMNRRRWLETAASVCLAACSCHSHSGGEQSDVSLSSKIRGT
                     LWWIDSNLDEASLRNEVYLQRNAGFDLLWLTGTQHLIKSPAGTSALEICLDQADRLAM
                     KVLFETYTTPDWYARWNLAEEVSLNRQFMTQVWERFRHHKSLYAWYIGHEIYLVWEKQ
                     "
BASE COUNT          293 a          303 c          318 g          262 t
ORIGIN      
        1 tgaagctcac ttcggggacg gtttgtgatc atcttgaggc tatactccca atgtggacag
       61 tcaacgaaat cagacaaaag gataaacacg cccccccggg gggtgcgaag agccaattcg
      121 gcaagcgctt ctttcatccc agtagttcct tcgggctcca acgacgccaa ttggtcgaat
      181 atcctgcaga aatgatcatt gccgcggctg ggtgggatgc cgacacacgt tccgttgttg
      241 aaagtccaga gtccgacacg ttcctgtgat gttaggccga tataggcaag cgacgcggcc
      301 acttggctgc tgtagtagtg cttcgtcggc ttgccacaac gcatggaggc acttgaatcc
      361 agcaccaggt gaatgtcaac gtcctcttca tcatggaaga ccttaacgaa caagcgaccc
      421 agacgcacgt agagattcca gtcaactcga cgcgggtcgt cgccctgaac ataagggcga
      481 tgatccttga agtccagacc gaaaccaggg cggtgcgacc agtgccctcc ctgcatctcg
      541 ccgagatgga tcttgcgaac cactgcggcg acacagtcca gtgcacgaat caaatccgga
      601 tccagaaatc tctttggcat acagaagccc ttttctgtaa tatcgtcatt gagactagca
      661 ttttcacccg cagagtaaaa gacaggagcg aaccatgtca caactgatga atcgaagaag
      721 gtggctggag acggcggcat cggtgtgcct ggctgcttgc agttgtcaca gtcattcggg
      781 cggagagcag tctgacgtga gtctgtcttc gaaaatcaga ggaactctat ggtggataga
      841 ctcgaatctg gatgaagcct ctttgagaaa tgaagtgtat ctccaacgta atgcgggatt
      901 tgatctgcta tggcttaccg gcactcagca cttgattaag agtcctgcag gcacgagcgc
      961 attggaaatc tgcctggatc aggcggatcg tcttgcgatg aaagttctct ttgagaccta
     1021 caccaccccc gactggtatg cacgatggaa tcttgcggaa gaggtctctc tgaatcggca
     1081 gttcatgact caagtgtggg agcggttccg ccatcacaag tcgctctacg cctggtacat
     1141 cggccatgaa atctacctgg tttgggaaaa gcaaag
//
LOCUS       DAOQUC010001500         1175 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_175168_length_1175_cov_3.730357, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001500 DAOQUC010000000
VERSION     DAOQUC010001500.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1175
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_175168_length_1175_cov_3.730357"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(87..>1175)
                     /gene="purH"
                     /locus_tag="PKH07_19440"
     CDS             complement(87..>1175)
                     /gene="purH"
                     /locus_tag="PKH07_19440"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: protein motif:HMM:NF002049.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HNT37174.1"
                     /translation="LSMLGKGEVSELMRLTLAAKAFRCTAHYDSAIFTYLQKVNEGAD
                     KKFPNVLFQEFEMQSTLRYGENPHQDAAIYASVPAESVSLRNARQIQGKELSYNNYVD
                     LESALSCAREFDEVSAVILKHNNPCGLAVAKTLAEAYQQAHDCDALSAYGGIVGLNRF
                     VDAETGKKLAEARFLECILAPGYAPEVLEMLAAKKNLRVLELPGMDKPRRQHALAFRG
                     IVGGLLVQDDDISIPSYDLKVVTEKRPTEEDLRSLLFAFRVCKLVKSNAIVLVQGTHT
                     VGIGAGQMSRVDSSELAAKKAGLKAQGAVLGSDAFFPFRDAVDAAAKAGARAIIQPGG
                     SMRDEEVIQAANEHGIAMVFTGRRHFRH"
BASE COUNT          270 a          342 c          297 g          266 t
ORIGIN      
        1 accgaagcaa acctagcaaa aagcactcga atccgtcaag aacttccgat gaattctttg
       61 tgtcttggtg gtgagacact cccagatcag tgccggaagt gtcttcgacc agtaaacacc
      121 attgcgatgc cgtgttcgtt ggctgcctgg atcacttcct cgtcacgcat ggaaccaccc
      181 ggttggatga tcgcacgcgc gcccgctttc gccgcagcgt caaccgcgtc acggaatggg
      241 aagaacgcat ccgagccgag tacagcacct tgcgctttga gccctgcctt cttggccgcc
      301 agttctgagg aatccacacg actcatctga cctgcaccga tgccaacagt atgcgttccc
      361 tgaaccagaa cgatggcatt ggatttgacc agtttgcaga cacggaacgc aaacagcagc
      421 gaccgaagat cctcttcagt cggtctcttc tctgtgacaa ccttcaggtc ataggatgga
      481 atactgatgt cgtcatcctg caccaacaac ccgccgacaa ttcctcgaaa tgccagagca
      541 tgctgccttc tcggcttgtc cattccagga agttccagca cacggaggtt cttcttggca
      601 gcaagcatct ccagaacttc cggagcgtaa cccggagcca gaatgcactc cagaaaccgt
      661 gcttcagcga gtttcttgcc tgtctctgcg tccacaaagc gattgaggcc gacaattcct
      721 ccataggctg acaaggcatc gcagtcatgc gcctgttgat aggcttctgc aagtgtcttc
      781 gcgacggcaa gcccgcaggg attgttgtgc ttgagaatca ccgcagacac ctcgtcaaac
      841 tctcgggcac aggaaagcgc ggattcgaga tccacgtagt tgttgtacga caattccttg
      901 ccctggatct gtcgggcatt tcgaagactg accgactcag caggaactga cgcatagatc
      961 gccgcgtcct gatggggatt ttcgccatag cgcagtgtag actgcatctc gaactcttgg
     1021 aacaggacgt tcgggaattt cttgtccgca ccttcattga ccttctgaag ataggtgaag
     1081 atcgccgaat cgtagtgggc agtgcatcga aacgcttttg ctgccagagt cagtcgcatc
     1141 agctcggaga cctctccttt tccgagcatc gagag
//
LOCUS       DAOQUC010001501         1175 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_175195_length_1175_cov_2.880357, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001501 DAOQUC010000000
VERSION     DAOQUC010001501.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1175
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_175195_length_1175_cov_2.880357"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1152)
                     /locus_tag="PKH07_19445"
     CDS             complement(<1..1152)
                     /locus_tag="PKH07_19445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37175.1"
                     /translation="MHYATMGADTWTLSTECPFPRSPTNTWHGQSSDAIQDAHLVTPA
                     LNIPPGCVLSFWHTYKFESTFDGGVIEISLDGGITWTDLGEHIISQNGYNAVISTDYM
                     SPIAGRTAWSGGTLGPMVEVRVDLSPFAGLLRLVRFRLASDAGNYREGWFIDDLKVEY
                     GEHCLSGNGRLAWDADGYSCQSEARLTLSDLDLAGAGVWTVSVFSTTEPSGEEIQMTE
                     TDDLGVFAAAVPLVGSSTPTEQDGLIQVSDFDTLTAFYLDARSLDGTANVPRTATASL
                     DCTPPTISDLSSYAVDESAVQISWKTDEPATSIVHYGLTEEAFDLQRVSPYLRSNHSI
                     LISGLTECTDLYFRVASSDSVGNTTVDDNAGANYSISTWEKANVFEDMLV"
BASE COUNT          275 a          336 c          312 g          252 t
ORIGIN      
        1 gacgagcatg tcctcaaaga cattcgcctt ctcccacgtg gagatgctgt agttcgctcc
       61 ggcgttgtca tctacggttg tgttcccaac gctgtccgaa gaagcgactc ggaagtagag
      121 gtcggtgcac tcggtgagac ccgagatcag aatgctatgg tttgacctca ggtacgggct
      181 gactcgctga agatcgaacg cctcttcagt caatccgtag tgcacgatgg acgtagccgg
      241 ctcgtccgtc ttccaggaga tctgaacggc cgactcatcc accgcatagg aggagagatc
      301 gctgatggtt ggaggcgtgc agtccaaaga tgccgtcgct gtccttggaa cgttggcggt
      361 tccatccaga gaccgcgcat ccagatagaa tgctgtcaac gtgtcaaaat ctgagacttg
      421 aatcaggcca tcctgctccg ttggtgtgct tgaaccgact agaggaaccg ctgcggcaaa
      481 gacgcccaga tcgtcggtct cggtcatctg aatctcctct cccgaaggtt ctgttgtact
      541 gaagaccgaa accgtccaga ctcccgcacc agccagatcc aagtcggaca aagtcaaccg
      601 tgcttcagac tgacaggaat acccgtccgc atcccaagcc aatcggccgt tgcccgagag
      661 acagtgctcg ccatactcga ccttgagatc atcaatgaac caaccttcac gatagttgcc
      721 tgcgtcactg gccagtcgga atcgaaccag acgaagcaag cccgcgaacg gactcaagtc
      781 aactcgaact tccaccatgg ggccgagcgt gccacccgac catgccgttc tgcccgcgat
      841 gggactcatg taatccgtgc tgatgacggc attgtaacca ttctggctga tgatatgctc
      901 accgagatct gtccatgtaa tgccgccatc aagcgagatt tcgatcactc caccatcaaa
      961 ggtggattca aatttatagg tatgccagaa actcaaaaca catcccggag gaatgttcag
     1021 agcaggtgtg acaaggtgag catcctggat ggcgtcgctg ctctgaccgt gccacgtgtt
     1081 tgtcgggctt cgtgggaacg ggcattcagt actcaatgtc catgtatctg cgcccatcgt
     1141 agcatagtgc atccatccgg acaccggagt gggat
//
LOCUS       DAOQUC010001502         1175 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_175233_length_1175_cov_2.479464, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001502 DAOQUC010000000
VERSION     DAOQUC010001502.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1175
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_175233_length_1175_cov_2.479464"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..775
                     /locus_tag="PKH07_19450"
     CDS             <1..775
                     /locus_tag="PKH07_19450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37176.1"
                     /translation="YRLLISSCADGVFFWWYPGGFRVGENSDYGIINADGTDREITRI
                     IRNNAKPFLNGPSRKPTDLWIEFDRDAYPEGVTGAYDVVKDVFWEAIEDGRTPGLRTV
                     GTGTTSANCPLLAVGNTKCTGTNPPKYLDGAFDLVEIQDANGKWVRVTKGSEIQVGKE
                     NPVTARVTITNLSEATWITEKTAKRTHVGTGAVSLIIDGHKSHKVPLSRDLPRHETLK
                     IRKVSLTERPITKPVDIVLLFEAEGRTRFGERFAIKLVP"
     gene            899..>1175
                     /locus_tag="PKH07_19455"
     CDS             899..>1175
                     /locus_tag="PKH07_19455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37177.1"
                     /translation="MKEKGKKKGRPAETILNGEQPTDSTPAKPLTNFKITSRSDREKR
                     VRIDSAWSRSNSLCERWFESSDALLARPLRWILWGIFYLFPNQSDSVR"
BASE COUNT          282 a          278 c          336 g          279 t
ORIGIN      
        1 ctatcggctg ttgatatcca gttgtgctga tggtgtcttc ttctggtggt acccaggagg
       61 ctttcgagtt ggggagaaca gcgactacgg gatcatcaac gcggatggaa cggatcggga
      121 gatcacgcgg atcattcgca acaacgccaa gccatttctc aatggccctt cccgaaagcc
      181 gaccgatctc tggattgagt ttgatcgcga tgcctatccc gaaggagtga ccggagccta
      241 tgacgttgtt aaggacgtgt tctgggaagc aatagaggat ggtcgaacgc caggcttacg
      301 gactgtgggt acgggcacca cttcagccaa ctgcccgctt ctggcggtcg ggaatacaaa
      361 gtgtacgggc acgaacccac cgaagtatct cgatggtgcg tttgacttgg tggagattca
      421 agatgccaat gggaaatggg tcagagtgac aaaaggttct gaaatccaag ttggcaagga
      481 gaatccagtc actgcgcgtg tgacgataac taacttgagt gaggcgacat ggatcaccga
      541 gaagacggcg aagagaacac acgttggcac gggtgcagtt tctctcatca tagacgggca
      601 taagagtcac aaggttcctc tgtccaggga tttgccacgt catgagacac tcaagatcag
      661 gaaggtttcg ctgaccgagc ggccaatcac aaagcctgtt gatatcgttc tgctgttcga
      721 agcagaagga agaacccgat ttggcgagag gtttgcaatc aaactggttc cctgagtcac
      781 ggtgcaggca tcttgtctgt tcacaactgt tcccgctgtg ttctcggtac acttgcacgg
      841 aacggccttt tgtgggatat atacgtccga ctgtttgaat gcatttggag gtttctcatt
      901 gaaggagaag ggcaagaaaa agggcaggcc tgccgagacg attttgaacg gtgaacaacc
      961 gaccgattcc acgccggcaa agccactgac aaatttcaag ataacatccc gttctgatcg
     1021 ggagaagaga gtccgaattg attcggcttg gtctcgctcc aactctttgt gtgaacgttg
     1081 gttcgaatcc tctgacgcct tgttggcaag gccgcttcgg tggattctgt gggggatctt
     1141 ctacctcttc ccgaaccagt ccgattccgt tcgtt
//
LOCUS       DAOQUC010001503         1174 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_175583_length_1174_cov_1.842717, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001503 DAOQUC010000000
VERSION     DAOQUC010001503.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1174
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_175583_length_1174_cov_1.842717"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..347
                     /locus_tag="PKH07_19460"
     CDS             <1..347
                     /locus_tag="PKH07_19460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37178.1"
                     /translation="TLMKLTSTGVYRVPRQSNSRQILSDTPIRAITDGDYLIGTFRHE
                     DGRRAILINNYDFSYTEWPTVTFDVPDDKVMEIDQQTGKEVPAIDDSPAVPGLQLSLD
                     SGAGRLFLLPPR"
     gene            376..>1174
                     /locus_tag="PKH07_19465"
     CDS             376..>1174
                     /locus_tag="PKH07_19465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HNT37179.1"
                     /translation="MPEYIVALDQGTTSSKAVLFDGQGKLLDIAQKEFEQIYPQPGWV
                     EHDPLEIWSSQREVFAHLLRSNQLHASQIRAIGITNQRETAIVWDRTTGQPVCNAIVW
                     QDRRTAPICQSLEQGGLSEYIRQNTGLVIDAYFSASKVKWILDNVEGAREKAQRGELL
                     FGTVDSWLVWNMTSGKVHATDYSNASRTMVYNIRERQWDTKLLEVLGIPLSMLPEVRD
                     SSGDFGEFEYDGSRIPIRGVAGDQQAALFGQTCLEDGMTKNTYGTGCF"
BASE COUNT          296 a          305 c          314 g          259 t
ORIGIN      
        1 ataccttgat gaaactcacg agcacgggtg tgtatcgggt gcctcgacaa agtaactcgc
       61 gacagatact ttcagatacc cccattcgag cgatcaccga tggagactat ctcatcggca
      121 ccttccgaca tgaggatgga cggcgagcca tattgatcaa caactatgat ttcagctaca
      181 cagagtggcc aacggtaact tttgatgtac cggatgacaa agtgatggag atcgatcaac
      241 agaccggcaa ggaagttccc gcgatagatg acagtccagc tgtcccaggg ctgcaactgt
      301 cgctagactc aggtgccggc agactctttc tactgccacc gagataatct gcggactgct
      361 caaaggactg aagtcatgcc ggaatacatc gtcgccttag accagggaac cacgagttcg
      421 aaagccgttc tgtttgacgg ccagggcaaa ctgctggaca tcgcccagaa agagtttgag
      481 cagatctatc cgcaacctgg ttgggtggag catgaccctc tcgagatatg gagttctcag
      541 cgtgaggttt tcgcgcatct tcttagatca aaccagttgc acgcctctca gattcgagcc
      601 atcgggatta ccaaccaaag ggagaccgcg atcgtctggg atcgaacaac gggtcagccc
      661 gtgtgcaacg ccatcgtgtg gcaagaccgt cgcaccgctc ccatctgcca gtctctcgaa
      721 caaggggggc tttccgagta catccgccag aataccggtt tggtcattga cgcctacttt
      781 tctgcaagca aggtcaaatg gattcttgat aacgtagaag gagcacgaga gaaggcgcaa
      841 agaggggaac tgctctttgg aactgtggac tcctggcttg tctggaatat gacttcaggg
      901 aaagtacacg ccaccgacta ctccaatgct tctcgaacga tggtctataa cattcgtgag
      961 agacaatggg acacaaaact tcttgaggtc ttgggtattc cactttccat gttacctgag
     1021 gtgcgagact catccggaga cttcggtgag ttcgagtacg acggcagccg gattccaatt
     1081 cgtggtgtcg ccggagatca gcaggctgct ctcttcggtc agacttgtct cgaagatggg
     1141 atgaccaaga acacctacgg cacgggatgc ttcc
//
LOCUS       DAOQUC010001504         1172 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_175900_length_1172_cov_3.236347, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001504 DAOQUC010000000
VERSION     DAOQUC010001504.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1172
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_175900_length_1172_cov_3.236347"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            35..>1172
                     /locus_tag="PKH07_19470"
     CDS             35..>1172
                     /locus_tag="PKH07_19470"
                     /inference="COORDINATES: protein motif:HMM:NF013662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37180.1"
                     /translation="MAIQAIIQGAIKGEVVESLLFRESKESLPLRCQRDLPFFRSQVR
                     SILGRNEKVDPVQIETYVAAGGYRALCCVLEKGNPRWVVEQIKTSGLRGRGGAGFPTG
                     LKWEMLAAQRNGSGKFLVCNADEGDPGAYMDRSVLEGNPNAIIEGMLIGAFGTGANLG
                     IIYVRNEYPLAIENLTLALQQARQLGVLGERILGTDFCFDISIVKGAGAFVCGEESAL
                     IRSIEGKTGEPRQRPPFPVERGIEGKPTAINNVETLANIPRIIEDGAESFAQTGAAKN
                     SGTKIFSLVGKIRNTGLVEVPMGIRIRDIVYDIGGGSATGSAIKAIQTGGPSGGCIPA
                     SQFDLSIDYDSLSQAGAMMGSGGMIVMDERTCMVDVAKYFMNFLK"
BASE COUNT          268 a          274 c          363 g          267 t
ORIGIN      
        1 gcctgagggt atcttctatc caaaggtccc gcgagtggcg atccaggcca tcatccaggg
       61 agcgatcaaa ggtgaagtcg tcgaaagcct tcttttcaga gaatccaagg agagtctgcc
      121 acttcgctgt cagagagatc tgccattctt caggagccag gttcggagca tccttgggag
      181 aaacgagaag gttgatcccg tccagataga gacctacgtc gctgctggtg gatatcgtgc
      241 cctgtgctgt gtacttgaaa aggggaaccc ccgttgggtg gtggagcaga tcaagacttc
      301 agggctgcgc ggtcgtggag gtgctggatt tccgacgggt ctcaaatggg agatgcttgc
      361 tgctcagcga aacggttcag gaaagtttct cgtctgcaac gcggatgagg gagatcctgg
      421 ggcatacatg gatcggagcg tcctggaggg gaatccaaac gccatcattg aagggatgct
      481 cattggtgct ttcggtaccg gtgccaacct ggggatcatc tatgttcgaa atgagtatcc
      541 gcttgcgatc gagaatctga ctctggcttt gcagcaggca aggcaactgg gcgtgcttgg
      601 tgagaggatt cttggaacag acttctgttt cgacataagc attgtcaagg gagcaggtgc
      661 tttcgtttgc ggcgaggaat ctgctctgat tcgttcgatc gaggggaaga ctggtgaacc
      721 acgccagcgt ccacctttcc ccgttgagag aggcattgaa gggaaaccaa ctgccatcaa
      781 caacgtggag acgttggcga acattccaag gatcatcgaa gacggtgccg aatcttttgc
      841 ccagaccgga gcagcgaaga actccggcac caagatattc agcttagtcg gcaaaatccg
      901 aaacacgggt ttggttgaag tacccatggg gattcggatt cgagatatcg tctacgacat
      961 tggggggggc tctgcgacgg gcagtgccat caaggcgatc cagaccggtg gaccctccgg
     1021 agggtgtatt ccggcttcgc agttcgatct tagcattgac tatgacagtc tttcccaagc
     1081 aggtgcgatg atgggatcgg gtggcatgat cgtcatggat gagcgcacct gcatggtgga
     1141 cgttgccaag tacttcatga actttctcaa gg
//
LOCUS       DAOQUC010001505         1170 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_176432_length_1170_cov_2.792825, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001505 DAOQUC010000000
VERSION     DAOQUC010001505.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1170
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_176432_length_1170_cov_2.792825"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..597)
                     /locus_tag="PKH07_19475"
     CDS             complement(<1..597)
                     /locus_tag="PKH07_19475"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HNT37181.1"
                     /translation="MVKDRPFVHLHVHSDFSVLDGALRISDAVAKASEWQMPALALTD
                     HGNLHGAISFYRTARKAGVKPIIGMEAYITPGRMSDKTKTEGRGGENLYHLMLLAENE
                     VGYRNLIALSSQAYLEGFYYKPRLDKELLALHADGLIAMTACLKGEVPSRIAESEFDK
                     ARTVCGQYCDIFGRDSLYLEVQENGIPDQRVVNEGLIKL"
     gene            complement(714..>1170)
                     /locus_tag="PKH07_19480"
     CDS             complement(714..>1170)
                     /locus_tag="PKH07_19480"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNT37182.1"
                     /translation="LVAFDTLQSAIQSNDTEAVRKKLEGDVDVNSQADNEQGFTALMY
                     AAGMGNQEIVSLLLAKNADPNISALDGSVPLHFAARAGNLEIVQMVLDKGADANAQDK
                     SGRTALAWAQKEQHDAVVTLLQGRTTAEAQKPEQVEETEEIKEPEQTEQ"
BASE COUNT          236 a          343 c          297 g          294 t
ORIGIN      
        1 gagtttgatc aaaccttcat tgaccacccg ttgatccgga attccgttct cctgcacttc
       61 cagatagagg ctgtctcttc cgaaaatgtc gcagtactgg ccgcagacgg ttctcgcttt
      121 gtcgaactcg gactcagcga tgcgagaggg aacctcgccc ttcaggcagg cggtcattgc
      181 aataagtccg tccgcatgaa gcgccagcag ctctttgtcc aggcggggtt tgtagtaaaa
      241 tccctcaaga taggcttgac tggacagagc aatcaggttt cggtatccaa cttcgttctc
      301 cgcaagcagc atcagatgat agaggttttc cccaccccga ccttccgtct tggtcttgtc
      361 gctcatcctg cccggtgtga tgtaagcttc catcccgatg atgggtttga ctcccgcctt
      421 gcgggcagtt cgatagaagg agatggctcc atgcaggttt ccgtggtcgg tcaaggccaa
      481 agcgggcatc tgccactcag aggccttggc gaccgcatca gaaatgcgca gcgctccatc
      541 cagaacactg aaatcactgt gcacatgcag atgaacaaaa ggtctgtctt tgaccatatc
      601 tcatcaaatc agagatgaaa tcctcgtctc tgatgctcta gaatcaaagg tcacggccag
      661 atcgcttccg acctgaccct cggttggcac tgcttgcctt ctacaagaaa aggctattgc
      721 tcagtctgtt ccggttcctt gatttcttcg gtttcctcta cctgttcggg cttctgagct
      781 tcagctgttg tcctgccctg caggagagtg accactgcat catgctgctc tttttgtgcc
      841 caagccagag ccgtgcgccc cgatttgtcc tgagcgttcg cgtccgcccc cttgtccaag
      901 accatctgga cgatctccag attgcccgca cgggcggcga agtgtagggg aacagaacca
      961 tccaacgcgc tgatattcgg gtcggcgttc tttgccagga gcagggaaac gatttcctgg
     1021 ttgcccatgc ccgcggcata catcaaagca gtgaagccct gctcattgtc cgcctgtgaa
     1081 ttcacgtcca cgtcaccttc cagcttcttt ctgacggctt cggtatcgtt gctctgaatc
     1141 gcgctctgga gcgtgtcgaa ggctaccaag
//
LOCUS       DAOQUC010001506         1170 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_176573_length_1170_cov_1.812556, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001506 DAOQUC010000000
VERSION     DAOQUC010001506.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1170
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_176573_length_1170_cov_1.812556"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..972)
                     /locus_tag="PKH07_19485"
     CDS             complement(<1..972)
                     /locus_tag="PKH07_19485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37183.1"
                     /translation="MTVAFAGSIVMQAEDAVLKTERSEIVEQASFNGGKGVSLKEGET
                     SNVGSLGTKADVVFRVRAANAGRYVIQTHAATDAKGTEIMKNAPSKHESMRLMIAVGE
                     ARPTSRVVFVPWSRPKSCYQTLGKFNLSGEEEEIRVWLPEGVRLDYVGINAYVALAVP
                     EAAAHYQPRVVPPSSRPRLWVNEKSLPQIRANLTKGENAPLWEEVKKQAAVPFEFSPE
                     PGVELSYDPALEKAVTLKAFVRLMSGDDELGREAISLVKDYLAAVEFGNLLDITREIG
                     RAIYAGAQVYDWCYDLMTEEERETIRLNLMRLADDMEIGWPPFKQMIV"
BASE COUNT          274 a          310 c          309 g          277 t
ORIGIN      
        1 gacgatcatc tgcttgaacg gaggccagcc gatctccatg tcatcggcga ggcgcatgag
       61 gttgagacgg atcgtctccc gttcttcttc cgtcatcaga tcgtaacacc agtcatatac
      121 ttgagcgcca gcgtagatgg ctcgtccgat ctcgcgcgtg atgtccagaa ggttgccgaa
      181 ctcgacagcg gcaagatagt cctttacaag cgaaatagcc tccctgccaa gttcgtcatc
      241 accgctcatg aggcgaacaa aggctttgag agtaactgct ttctcaagag cagggtcata
      301 gctgagttca acgccaggtt ccggagaaaa ctcaaatggc accgccgcct gtttcttgac
      361 ttcctcccag agtggcgcat tttcaccctt tgtgaggttg gctcgaatct ggggaagtga
      421 cttctcgttg acccacaggc gtggtcgaga tgaaggcgga acgactctag gttgatagtg
      481 agccgctgct tccgggacag cgagggcaac gtaggcgttg attccgacgt aatcgagcct
      541 aacaccctca ggaagccaaa ctcgaatctc ctcttcttct ccactcagat tgaacttgcc
      601 caatgtctga tagcagctct tgggacggct ccaaggcacg aagacaactc gactggtcgg
      661 acgtgcttca ccgacggcaa tcatcagtcg catggactca tgtttggatg gcgcattctt
      721 catgatctca gtccctttgg cgtctgttgc cgcgtgggtc tggatcacat accgtccggc
      781 attggcagca cggactctga agaccacatc tgccttcgtg ccgagcgaac cgacgttgga
      841 tgtttcaccc tctttcaacg aaacgccctt gccaccgttg aatgatgcct gttcaacgat
      901 ctccgagcgt tccgtcttga gaacagcatc ttccgcctgc ataactattg atccggcaaa
      961 cgcaacggtc attgatggct cctgggctgc gggggatgtt tgaactacga tgacaagcag
     1021 cagtgaattg agaagaatcg gtaagtgttg tttcatggag agagaatgcc agacggctga
     1081 agaaagatca agaattcgag acacagatgt ggctaagtta cttcccgttt gacaggaaca
     1141 tgataggcgt catagaattc cctgaatgat
//
LOCUS       DAOQUC010001507         1168 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_176946_length_1168_cov_2.572327, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001507 DAOQUC010000000
VERSION     DAOQUC010001507.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1168
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_176946_length_1168_cov_2.572327"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1168
                     /locus_tag="PKH07_19490"
     CDS             <1..>1168
                     /locus_tag="PKH07_19490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37184.1"
                     /translation="SVGIFRIQPVSIFMMDYYGQFHYDKVGYQPNVPLNFGWDIARSV
                     KVDPGQSNGYYVVDAFGGLYAFGLPNLGSLYFSQDGVKDIEIVEGGGYYILNGYGLVD
                     CVGGAKFYGDPPFTGQDRYLDLEPTVTGRGYYVLDVAGGIHTFGDATPFAAPLMQQGA
                     TAVDIELTPDGTGMYLLDSRGAVHALGTAEYHGEVYMGLDVARDLLVTSEGEGYVVLD
                     AYGSTYRFGVAPMVQLVTEELVPPAAKDLEFGRGYIAPDRRVIARLDSQYAQPDVSAD
                     YVVWSANNGAQGKDLFLYDIVLDATRLLVSATKDQLNPAINGDKVVYTTIQTGRKEIY
                     GVTIPSGTQEAVPFVIATSGVQQEQPDISADYVVWSAYGGVLANRSFGGAQVSRT"
BASE COUNT          231 a          298 c          358 g          281 t
ORIGIN      
        1 ctccgtcggc atcttccgta ttcagcctgt ctccatcttc atgatggact actacgggca
       61 gttccactac gacaaggtcg gttatcagcc aaatgtgcct ctgaactttg gctgggacat
      121 tgcgcgaagc gtgaaagtgg atccgggtca gagcaatggc tattacgtgg tggatgcgtt
      181 tggcggcctg tatgcgtttg gtctgccgaa tctcggttcc ctttacttct cccaggatgg
      241 agtcaaagat attgaaatcg tagagggtgg tggttactac attctcaacg gctacggact
      301 cgttgactgt gtgggtggcg ccaagttcta cggggatccg ccattcacgg gtcaagatcg
      361 atacttggat ctggagccaa cagtgacggg acgaggctac tatgttctgg atgtggctgg
      421 tggcatccac acctttggtg atgcgacccc gtttgcagcg ccgctgatgc agcagggagc
      481 aactgctgtg gatatcgaac tgacaccgga tggtacagga atgtatctct tggacagccg
      541 tggtgcggtt catgcgttgg gtacggctga atatcatggc gaagtctaca tggggctgga
      601 cgtggctcga gacctgctgg tgacctcaga gggcgaaggg tacgttgtcc ttgatgccta
      661 tggcagcacc tatcgctttg gcgttgcccc gatggttcaa ctggtcactg aggagttggt
      721 gccgcctgcg gcgaaggatc tcgagtttgg tcgcggctat atcgctcctg acagaagagt
      781 catcgccagg ttggacagtc agtatgccca gccggatgtg tcggcagact atgttgtctg
      841 gagcgccaac aacggagccc agggcaaaga tctgttcctg tatgacattg tgcttgatgc
      901 cacccgcttg ctggtgtcgg cgaccaagga tcagctgaat ccggcgatca atggtgacaa
      961 ggttgtctac accacgattc agacgggcag gaaagagatc tatggtgtga cgatcccctc
     1021 cggcacgcaa gaagcagttc cattcgtcat tgcgacatct ggcgttcagc aggaacagcc
     1081 cgacatttcc gccgactatg tggtctggag tgcttacggc ggcgtcttgg ccaaccgctc
     1141 cttcggcggc gcccaggtgt cccgcacc
//
LOCUS       DAOQUC010001508         1167 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_177205_length_1167_cov_2.702338, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001508 DAOQUC010000000
VERSION     DAOQUC010001508.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1167
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_177205_length_1167_cov_2.702338"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            106..528
                     /locus_tag="PKH07_19495"
     CDS             106..528
                     /locus_tag="PKH07_19495"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HNT37185.1"
                     /translation="MIQNMPVEMKLGELSERSGIPARTIRLYVSKGLLPGPTRPGPGA
                     TYTREHLQLLDRIRQLQRQGMTLVEIRGRLCVREPEKSIAKAEAWMVYDLDSDVRVMV
                     RAESSPWRLRKLQRALGRFQKEIKDDENGNAERDGRDL"
     gene            485..>1167
                     /locus_tag="PKH07_19500"
     CDS             485..>1167
                     /locus_tag="PKH07_19500"
                     /inference="COORDINATES: protein motif:HMM:NF020076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT domain-containing protein"
                     /protein_id="HNT37186.1"
                     /translation="MMRTGMQNVMEEICNNYCVRNPKSGEECKLAMQELWLTGRLLPL
                     GARLVVRHVFRSGETKPMEMVYSFALPRDASLSRFKVTGKSFSVESELKDTQQAVKDY
                     EQGVEEGHLGVMARSYRDGVVNLTLGNILPDEEVVVLLEMVAGVQLQDDGYRFRFPFT
                     LAPTYHAQARTVVNENGMGEMELPEEFGDVILPVWSQDASALHRVGFDLSVEACGDLL
                     EVSSPSHTV"
BASE COUNT          268 a          267 c          353 g          279 t
ORIGIN      
        1 gtgtcactag gagaacctca gactcgaatt gtgttggctt ttctctaaga attatcttga
       61 cactgtcaaa tagtgttata tgctcctgcc tggaggtgaa ggactatgat tcagaacatg
      121 ccagtggaga tgaaacttgg cgagctttcc gaacgttcag gcatcccagc aagaacgatt
      181 cgtctctatg tgtccaaagg cctgttgcca ggacccacac gacctggccc tggagcgacc
      241 tatacacgtg agcacctgca actgttggat cgaatcagac agctccagcg gcagggcatg
      301 acgctggttg agattcgcgg gcggctgtgc gttcgggagc cggagaagag tattgccaag
      361 gcggaagcat ggatggtcta tgaccttgat tcggacgtca gggtcatggt tcgagcagag
      421 agcagtccgt ggcgcctcag aaaactgcag cgggcgctcg gaagatttca gaaggagatc
      481 aaagatgatg agaacgggaa tgcagaacgt gatggaagag atttgtaaca actactgcgt
      541 caggaatccg aagagtgggg aagaatgcaa actcgcgatg caggaacttt ggctgacagg
      601 gcgtttgctc cctttgggtg cccgtcttgt tgttcggcat gtgtttcgtt cgggtgagac
      661 caagccgatg gagatggtct acagttttgc gctccccagg gatgcatccc tcagccggtt
      721 caaagtcacc ggcaagtcct tctcggtcga gtctgagttg aaagacacac agcaggcggt
      781 caaggactat gaacaaggcg tcgaagaggg tcatcttggg gttatggcgc gttcctacag
      841 agacggagtt gtgaatctga cgcttggaaa cattcttcct gacgaggaag ttgttgtgct
      901 ccttgagatg gttgctggag ttcaattgca ggatgacggg taccggtttc gattcccctt
      961 tacgctggct cccacctatc acgcacaggc acggactgtg gtgaatgaga acggtatggg
     1021 tgagatggaa ctccccgaag aattcggaga tgtgatcctg cctgtctggt ctcaagatgc
     1081 ttcggcactg catcgggttg gttttgatct gtctgtcgaa gcctgtggag atctcctgga
     1141 agtttcatcg ccgtcgcaca ccgttca
//
LOCUS       DAOQUC010001509         1166 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_177587_length_1166_cov_2.015302, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001509 DAOQUC010000000
VERSION     DAOQUC010001509.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1166
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_177587_length_1166_cov_2.015302"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1166)
                     /locus_tag="PKH07_19505"
     CDS             complement(<1..>1166)
                     /locus_tag="PKH07_19505"
                     /inference="COORDINATES: protein motif:HMM:NF014788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HNT37187.1"
                     /translation="EMEVIAQTWSEHCKHKVFSSMIEYQEQGKDSELIDGIFHQYIAQ
                     ATRTVRQAKGSDDYCVSVFLDNAGVISYDKDCCVAFKVETHNHPSALEPYGGAGTGIG
                     GVIRDVLGTGLGARPCFNTDIFCFAPPDWPYDKLPEGVLHPRRILRGVVSGVRDYGNR
                     MGIPTVSGGIQFDEKFLGNPLVYCGTAGVIPTKRMKKGCRAGDMLVAIGGRTGRDGLH
                     GATFSSLDLHKDTPTSTVQIGNPIVEKKVTEALVRARDEGLLRDVTDCGAGGFSSAIG
                     EMAKEHGAVVFLEKAPLKYSPLEDWEIFLSESQERMVAAIAPEHFNQLKKICQIEEVE
                     VVSLGQVTNSHRLVVWHDGQVVGDLAMSFLHKGVPRQKRKAIWQPKQLPRCEVV"
BASE COUNT          267 a          353 c          283 g          263 t
ORIGIN      
        1 tcaccacttc acatcgcggc agttgtttgg gctgccagat cgccttgcgc ttctgccgcg
       61 gcactccctt gtgaagaaac gacatggcaa gatcaccgac cacctgaccg tcatgccaga
      121 ccaccaggcg atgtgaattg gtcacctgac caagcgaaac cacttcaact tcctcaatct
      181 gacagatctt cttcaactgg ttgaagtgct ccggtgcaat ggcagcaacc atccgctcct
      241 gagattccga gaggaatatc tcccagtctt ccagtggaga gtatttcaga ggtgccttct
      301 ccagaaagac gacggcaccg tgctcttttg ccatctcacc gatggccgaa gagaatcccc
      361 ctgcaccaca gtcagttaca tcacgcagga gtccttcatc ccgagcacgg acaagagcct
      421 cggtgacctt cttctccaca atcgggttcc cgatctgaac ggtgcttgtc ggcgtgtcct
      481 tgtgcaggtc gagcgaggag aatgttgctc catgcagacc gtctcttcct gttcttccac
      541 cgatggcgac cagcatatca ccggcacgac aacccttttt catgcgtttg gtgggaatga
      601 cccctgccgt tccgcagtaa accagaggat tacccaagaa cttctcatcg aactgaatgc
      661 caccgctgac ggtgggaatc cccattcgat tcccatagtc gcgaacaccg gagacgacgc
      721 ctcgaagaat gcggcgcgga tgcaaaactc cttcagggag tttgtcgtat ggccagtcgg
      781 gcggcgcgaa acagaaaatg tctgtattga agcacggcct cgctcccaga cccgttccca
      841 acacatcccg aatcacacca ccaataccgg ttcccgcacc accatagggc tccagcgcgg
      901 aaggatggtt gtgggtttcc accttgaacg ccacgcagca gtccttgtcg tacgaaataa
      961 caccggcatt gtcgagaaat accgaaacac agtagtcatc agatcccttt gcctgtcgaa
     1021 cagtgcgggt tgcctgggca atgtattgat gaaagatgcc gtcaatgagt tccgagtcct
     1081 tgccttgttc ctgatattcg atcatggaac tgaagacttt gtgtttgcag tgctctgacc
     1141 acgtctgcgc gatgacttcc atctcc
//
LOCUS       DAOQUC010001510         1164 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_177976_length_1164_cov_3.131650, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001510 DAOQUC010000000
VERSION     DAOQUC010001510.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1164)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1164)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1164
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_177976_length_1164_cov_3.131650"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1164
                     /locus_tag="PKH07_19510"
     CDS             <1..>1164
                     /locus_tag="PKH07_19510"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNT37188.1"
                     /translation="LENLRHNPHSNFSIWDTILYEKLHYAPNVTLLLNCSCLDADMKG
                     RRIKSITGWQLTTQMYHQVSASIYADCSGDAILAPLTKAPFRMGREARSEYNESIAPE
                     QADNKTMGMTCLFMARECDTPQPFEPPSWAYRYDNEEDIPRGAKGHGWWRMGYWWIEL
                     GGEFHSIHDTEMLRDELLKTVYGVWDHIKNRGDHGADNWTLEWIQFLPAKRESRRYIG
                     EHVLTQNDIESEGKFEDIVAYGGWAMDDHHPAGFRAAKLGAPATIFYPTPSPYGIPYR
                     SLYSKTVENLMFAGRCASCTHSAMSSSRVMGTGCSMGQAVGTAAAMAVSKNCPPKGLT
                     GECIRQLQQTLLVDDCYLPGIVQQLPTATLRAQLVASQGDPEPVRDGINRQVG"
BASE COUNT          278 a          318 c          320 g          248 t
ORIGIN      
        1 tctggagaat ctccgacaca atccgcattc gaatttctca atctgggaca ccattctcta
       61 tgagaaactc cactatgcac ccaatgtgac gcttctgctg aactgctcgt gtctggatgc
      121 cgacatgaag ggtcgtcgaa tcaagagcat cacgggctgg cagttgacga cgcagatgta
      181 tcatcaggtc tccgcgtcca tctacgcgga ctgttcggga gatgccattc ttgctccctt
      241 gaccaaagcc cctttccgaa tgggtcggga agccaggagc gaatacaacg aatccattgc
      301 acccgaacaa gcggacaata agaccatggg catgacgtgc ctgttcatgg cgcgggaatg
      361 cgatacgcct cagccatttg aaccgccatc gtgggcgtac cgttatgaca acgaagagga
      421 tattccacgc ggagccaaag gccatggctg gtggcgaatg ggttattggt ggattgaact
      481 gggtggtgag tttcactcaa ttcatgacac cgaaatgctt cgtgacgaac ttctgaaaac
      541 cgtctacggc gtttgggatc acatcaagaa tcgaggcgac cacggagcgg acaactggac
      601 gctggaatgg attcagtttc tccctgccaa acgagaaagc cgccggtaca ttggcgagca
      661 tgtgctcacg cagaacgata tcgaatccga aggcaaattt gaagacattg ttgcctacgg
      721 tggttgggca atggacgacc accatcctgc tggattccga gccgccaaac tgggcgctcc
      781 ggcaaccatc ttctatccca ctccatctcc ctatggaatt ccctaccgat cgctttattc
      841 aaagaccgtc gagaacctca tgtttgcggg acgttgtgcc agttgcaccc actcggccat
      901 gagttcgtcg cgtgtcatgg gtaccggttg cagcatgggt caggcggtcg gtactgcagc
      961 agcgatggca gtctcgaaga actgcccacc aaaagggctg accggagagt gcatcagaca
     1021 acttcaacag acgctcctgg tggacgactg ctatcttccc ggcatcgttc agcaactccc
     1081 gacagctacc ctgagggctc agttggtggc gagtcaggga gatccggaac cggtgcgtga
     1141 tggaatcaac cgacaggttg gcga
//
LOCUS       DAOQUC010001511         1161 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_178723_length_1161_cov_3.627486, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001511 DAOQUC010000000
VERSION     DAOQUC010001511.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1161
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_178723_length_1161_cov_3.627486"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1161
                     /locus_tag="PKH07_19515"
     CDS             <1..>1161
                     /locus_tag="PKH07_19515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37189.1"
                     /translation="TFLDDDGNASFHLRQGDCEVARHDAMLECAAADIVAMCEYLLTT
                     RDRVEIFKNIRLLERSADFLDSRRDPDNGLFRAGPASNLLAPSYAGTRMEDGSYSWGY
                     LAGLQVNYCAALSRLIEVEKLAQQADAVKRYSARLDAALKALPQLMTEGGYLVKYIDQ
                     FGVKHGVYGAEKFGYMEGTCNVDAIAFGVLDLQASHRAYAAMKAVAGLWYKGLIRNNY
                     PILDDTYDHWGTKEPKELLWRYGFWVDGGVWGTNVARMVLAQYRLNDFDGVWASLSTI
                     MAEMRQGTANAIWISTDMGTSGRPNELDTYAVPGAALRGLWECRYDAESLTIVPHVPP
                     GIKHFRQKQPLRFGKKRIYLEIENEGSRLLSAKVNGDVASVTDRLRLPYVSL"
BASE COUNT          251 a          303 c          350 g          257 t
ORIGIN      
        1 gacatttctc gatgacgacg gcaatgccag ttttcacctc cgacagggag actgcgaagt
       61 ggcacgccat gacgccatgt tggagtgcgc cgccgcggac attgtcgcca tgtgtgagta
      121 tcttctcacc acgcgggacc gcgttgagat attcaagaat atccgtcttc tggagcgttc
      181 ggcggacttc ctcgattccc gaagagaccc ggacaacggg ctctttcggg caggcccagc
      241 atcaaacctt cttgccccat cgtatgcagg aacccgcatg gaggacggtt cttacagttg
      301 ggggtatctg gcagggctcc aggtaaacta ttgcgccgcg ctttctcgac tcattgaagt
      361 tgagaagttg gcgcaacagg cggacgctgt gaagaggtat tccgcacgct tggacgccgc
      421 cctgaaggct ctcccacaac tcatgaccga gggcggctat ctcgtgaagt atatcgacca
      481 gttcggagtg aaacacggtg tctacggtgc tgagaaattc ggttacatgg agggtacctg
      541 caatgttgac gccatcgctt tcggcgttct tgatctccaa gcgtcgcata gggcatacgc
      601 ggcgatgaaa gccgtcgccg gtctttggta caaggggctg atccgcaata actatcccat
      661 cctggatgat acctatgacc attggggaac gaaggagccg aaagaactcc tgtggcgcta
      721 cggattctgg gtcgatgggg gagtctgggg gaccaatgtg gctcggatgg ttctggcaca
      781 gtatcggttg aatgacttcg acggcgtgtg ggcttcattg agcaccatca tggcggagat
      841 gcggcaggga actgcaaacg ccatctggat ttcgaccgac atgggcacat caggaagacc
      901 aaatgaattg gacacctatg ctgttcctgg cgcggctctg cgagggcttt gggaatgtag
      961 gtatgacgct gaatcgctga cgatcgttcc ccatgtaccg cccggcatca agcattttcg
     1021 tcagaagcaa cctctgcgtt ttggcaagaa gcggatctat ctggagattg agaacgaagg
     1081 gagcaggctg ctctccgcga aggtgaacgg cgacgtggcc tccgtgacgg acagacttcg
     1141 cctgccctac gtttctctaa g
//
LOCUS       DAOQUC010001512         1160 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_179072_length_1160_cov_2.502262, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001512 DAOQUC010000000
VERSION     DAOQUC010001512.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1160
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_179072_length_1160_cov_2.502262"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..>1160
                     /locus_tag="PKH07_19520"
     CDS             2..>1160
                     /locus_tag="PKH07_19520"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HNT37190.1"
                     /translation="MSKKLHVAFLWHQHQPYYRLPHSNTLTQPWVRLHGLKDYLDMPL
                     RLEEFPRIHQTFNLVPSLLEQLDSYAGNKVTDPFLELSRKRASMLEDEDKLELLKTHF
                     SGNVKTMIAPFPRYFSLHRRISSRTFEESLSRLQESDYRDIQVWFNLAWLDPMFRDVD
                     DVPRSLVQKGEDFSEEDKQRLFDYEDSILRRIIPTHKDLQEQGLIEVSTTPFFHPILP
                     LLCDFRSALESEPHAVLPSVEFRHPEDALAQIQMGIELYRSYFGRPPLGMWPSEGSVS
                     PQAVRLIADAGINWIATDEEILSLSLKKPFRRDSNGLLESPTLLYRPYLLDDTQGRLT
                     CIFRDHTLSDLVGFQYYHMEPKKAAKDLVQRLENIHDQGLTDEHLVSIVLDG"
BASE COUNT          275 a          307 c          281 g          297 t
ORIGIN      
        1 tgtgtcaaag aaacttcatg ttgcctttct gtggcatcag catcagccgt actaccggtt
       61 gccacattcg aacaccctca ctcaaccctg ggttcgcctt catgggctga aggactattt
      121 ggatatgccg ctccgacttg aggagtttcc tcgaatccat cagaccttca acctggttcc
      181 atctttgctc gagcaactcg attcctatgc gggcaacaaa gtcactgatc cctttcttga
      241 gctttctcgc aaaagagcaa gcatgcttga ggacgaggac aaactggaac tcctcaagac
      301 gcatttcagt ggaaatgtca agacgatgat cgctccgttt ccccgatatt tctcgctcca
      361 cagacgaatc agcagccgca cttttgagga atccctgagc cgactgcagg agtctgatta
      421 cagagatatt caggtctggt tcaacctcgc ctggttggat ccaatgtttc gtgatgttga
      481 cgacgtaccg cgctctctgg ttcagaaagg cgaagatttc agcgaggagg acaagcagag
      541 gctttttgat tatgaggact ccattcttag gagaatcata ccaacgcaca aagatctcca
      601 ggaacagggt ctcattgaag tctcgacaac ccccttcttt catccaatcc ttccgctttt
      661 gtgcgacttc cgttctgcat tggagagtga gccgcatgca gtcctgccat cggttgagtt
      721 tcgacatccc gaagatgctc tggcacagat tcagatgggc atcgaattgt atcggtcgta
      781 ctttggaaga ccaccacttg gcatgtggcc atcggagggg agtgtcagtc cgcaggccgt
      841 gcgtcttatc gcagatgctg gcatcaactg gattgcaacc gatgaggaga ttctctcact
      901 gtctctcaaa aaaccgtttc gaagagatag taacgggttg ctcgagtccc ccacgcttct
      961 ctaccgtcct tacttgctcg atgacacgca gggcagactt acctgtatct tcagagacca
     1021 tactctgtct gatcttgtcg gttttcagta ctaccacatg gagcccaaga aggctgcgaa
     1081 ggatttggtt caaagactgg agaacataca cgatcaggga ctcacagacg agcatcttgt
     1141 cagcatcgtt ctggacgggg
//
LOCUS       DAOQUC010001513         1158 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_179608_length_1158_cov_2.407072, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001513 DAOQUC010000000
VERSION     DAOQUC010001513.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1158
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_179608_length_1158_cov_2.407072"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1158
                     /locus_tag="PKH07_19525"
     CDS             <1..>1158
                     /locus_tag="PKH07_19525"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HNT37191.1"
                     /translation="DPEFRAMWVSRFEWPNSNRTTCQNKINEIMTNLADHNFNAVVFQ
                     IRGQMDTLYPSPHEPWSALLGSLGSDPGWDPVQYAIDAAHSRGLEFHAYINYSVCWQS
                     STNSPPAHTTPEHPFWLHCNTSGTSDWLLANSSGTQVVYGSDGYVWVANGVPSFMEWI
                     RKVTMHVVNTYDVDGIHYDRIRFPANTYSYDTISNARRAGPGNPHSLGFKDWQRDQIT
                     RSLTDIYGSIMKVKPWIKVSNAPFGIYDRSRFAGYSGYTDAYDIWLQESQHWSEVGCM
                     DAVIPMIYWDIATAPRYDILIQDWVDHNYGRHIYGGMSASKYTFPELGNEMLENRNRG
                     AQGHCPFSYGSISASEFDTFLSTYFQTKVDTPSMPWKDNPTTGIIQGIVRDG"
BASE COUNT          265 a          307 c          303 g          283 t
ORIGIN      
        1 gatcctgagt ttcgggcgat gtgggtatct cgttttgaat ggccaaacag caacagaacc
       61 acctgccaga acaagatcaa cgagatcatg accaatctgg cagatcacaa tttcaacgct
      121 gttgtctttc agattcgcgg gcagatggat acgctttacc cttcgcccca cgaaccttgg
      181 tcggccttgc tgggaagtct cggatcggat ccaggctggg atcccgtgca atatgccatt
      241 gatgcagcgc attcaagggg tttggagttt catgcataca tcaactactc ggtctgttgg
      301 cagtcttcca ccaacagtcc gcctgcccac acgacgccgg agcacccctt ctggcttcac
      361 tgtaacacat ctggaacgtc ggactggctt ttggccaatt catcgggaac tcaggttgtc
      421 tatggttctg acggctacgt ttgggttgcc aatggtgtcc cttcattcat ggagtggatt
      481 cgtaaggtga cgatgcacgt cgtcaacact tacgacgtgg atggcattca ctatgaccgc
      541 attcgatttc cggccaacac gtactcctat gacaccatca gcaacgcgcg tcgggcgggt
      601 cccggaaatc cccacagcct tggtttcaag gactggcagc gggatcagat cactcggagt
      661 ctcactgata tctatggttc gatcatgaag gtcaaaccat ggattaaggt cagcaatgca
      721 cccttcggta tctatgatcg atcgaggttt gcgggttatt cgggttacac ggatgcctac
      781 gatatctggt tgcaggagtc ccagcactgg tcagaggtgg gttgtatgga tgctgtcatc
      841 ccgatgatct actgggacat tgcgacagcc cctcgctacg acatcctcat tcaagactgg
      901 gtggatcaca actacggacg ccatatctat ggtggaatgt ccgcctccaa gtacaccttc
      961 ccggaacttg gaaatgagat gcttgaaaac cgaaaccgtg gtgctcaagg acactgtccg
     1021 ttttcctatg gtagcatcag cgcgtcggag tttgacacct tcttgagcac ttatttccag
     1081 acgaaggtgg acacgccgag catgccttgg aaagacaatc cgaccacagg catcatccag
     1141 ggaattgtgc gggatggc
//
LOCUS       DAOQUC010001514         1157 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_179778_length_1157_cov_3.529038, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001514 DAOQUC010000000
VERSION     DAOQUC010001514.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1157
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_179778_length_1157_cov_3.529038"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            452..>1157
                     /locus_tag="PKH07_19530"
     CDS             452..>1157
                     /locus_tag="PKH07_19530"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNT37192.1"
                     /translation="MLKKIAVANQKGGVGKTTTSVNLACYLAMEGRRVLLVDLDPQGN
                     STSGLGLDRHSLQKTVRNVLSESARIADVLCLTPIRGLDLVASNNDTTEAEVFLLRQP
                     EGKSRLKEALDEYSVSVQSKSSPNSSEANVDYVIMDCPPSLGLLTVNALMAADSLLLP
                     VQCEYYAMEGLTDLLRTFTAIKQEFNPALRLEGIVLTMADARTNLSKEVGEEIRHHYG
                     RHVFETAIPRSVRLSEA"
BASE COUNT          263 a          309 c          315 g          270 t
ORIGIN      
        1 ggctcgatta cgatccaggc gcataatctc accgagcaga tgatcgtcac ccacttcaac
       61 cagttccaac atgtgcgctt gggtcacacc ctcagcctgc acaacaggcc cactgacccg
      121 gctcactgtt ccaatgatct gcttcaaatt ccatctccca ttgtggagtc gcttctctgt
      181 tcatgaatag attccggcaa aggacttgtg cccttttaga gaagaatagc acacttgcgc
      241 gtccggggga aacggaacaa gagttgacgt ggcattcgtg atagccatca gaatctactc
      301 attgggtcat tctgggcagg ccagtgcgtg caggcgtgac agttggcact gtcacaaggc
      361 ctatggcaaa taccgctacc ctcatttgcg cttgctcgcc aaacgtgcta tctgatagcg
      421 tttctcggat attgttgagg agtctcgaat tttgctcaaa aagattgctg tcgcaaacca
      481 gaagggtggc gtcggaaaga cgaccacaag tgtcaacctc gcctgctatc ttgccatgga
      541 aggaaggcgt gtgctcctgg tggacttaga tcctcagggt aactcgacca gcggactggg
      601 cttggacagg catagccttc agaagaccgt tcgcaacgtg ctgagcgaat ctgcccgaat
      661 tgccgacgtg ctgtgtctga cgccgattcg ggggctcgat ctggtggcgt ccaacaatga
      721 cacgaccgag gcagaggtct ttcttctcag acagcccgaa ggaaagtctc gcctgaaaga
      781 agcgctcgac gagtactcgg tttcggttca gtcgaagtca tcacccaatt catctgaagc
      841 gaatgttgac tacgtcatca tggactgtcc gccctctctg ggactgctga ctgtgaatgc
      901 actgatggct gccgacagtc tgcttctgcc ggttcaatgc gaatactacg ccatggaagg
      961 gctgaccgat ctgcttcgga catttacggc gatcaagcag gagttcaacc cggcgcttcg
     1021 tctggaggga attgtgctca cgatggcaga tgcccgcacc aacctttcca aggaagttgg
     1081 cgaggagatt cggcatcact atgggcgtca tgtttttgag acggctatcc caaggagtgt
     1141 tcggctgagt gaggcaa
//
LOCUS       DAOQUC010001515         1157 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_179873_length_1157_cov_2.528131, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001515 DAOQUC010000000
VERSION     DAOQUC010001515.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1157
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_179873_length_1157_cov_2.528131"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..611
                     /locus_tag="PKH07_19535"
     CDS             <1..611
                     /locus_tag="PKH07_19535"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HNT37193.1"
                     /translation="GLYYFHARWYDPEVGRFVSKDTGLFSKNLYSFASGNPVLFYDPD
                     GRETVLGLYIPSYVTLSGSFLQNKNRKPCPCNRSDLPLINDYIRFYQDMVQWYKNPEK
                     HPEPKSPLGGEPGRAAHTDCTGGPTEGMATVYIDAPFEKDCFNMCLLKHEIAHAEQCH
                     SMGMTQYYATYRKRTGEATMETAAYNSQISCLQGEKNRILGR"
     gene            608..952
                     /locus_tag="PKH07_19540"
     CDS             608..952
                     /locus_tag="PKH07_19540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37194.1"
                     /translation="MNQWTVTPQFRRVARILVSSCVVVLVLMAIPLLVLELLTKPNTV
                     VTAFPVGGFYVGIGFCSKWQQTYWHSSSGLPVTYLGYQGWVHLQQGRYMWSKWGVTIF
                     VYDDIIFLGDLE"
     gene            964..>1157
                     /locus_tag="PKH07_19545"
     CDS             964..>1157
                     /locus_tag="PKH07_19545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37195.1"
                     /translation="MPSLSRVAAFNSLRAASRCDTGIRARDLLFCDTDKSVCFTLTWV
                     RRASVRESVCGAGILPAKCFS"
BASE COUNT          279 a          263 c          305 g          310 t
ORIGIN      
        1 ccggtctcta ctacttccac gcccgctggt acgatccgga agtggggagg tttgtatcaa
       61 aggacactgg gctctttagc aaaaaccttt acagttttgc cagtggaaac ccggtgctgt
      121 tctatgatcc agacggtcgc gagactgtgc ttggtttgta cataccgagt tatgtcactc
      181 tcagtgggtc attcttgcag aataagaacc gcaagccttg tccatgcaat aggagtgatc
      241 ttccgttaat taacgactac atccgattct accaggatat ggtacaatgg tataagaatc
      301 cagagaaaca tccagagccc aagagcccgc tagggggaga acccggcaga gcagctcaca
      361 ccgattgcac aggaggtccg actgaaggca tggctacagt ctacattgat gcaccatttg
      421 agaaggattg tttcaatatg tgcctattaa agcatgagat tgcgcatgcc gaacaatgcc
      481 acagtatggg aatgacacaa tactatgcaa cctataggaa gaggacggga gaagcgacca
      541 tggagaccgc tgcatacaac agtcagatct cctgtttgca gggcgagaag aataggattc
      601 tgggaagatg aaccagtgga cagtcactcc gcaattcaga cgcgtcgcac gaatcttagt
      661 gagttcctgt gtggttgttc ttgttctcat ggcaattcct ttattggttc tggaactcct
      721 tacaaagcca aatacggtag tgacggcgtt tcctgtaggt gggttctatg ttgggattgg
      781 cttctgttcc aagtggcaac agacatattg gcactcctct agtggcttgc cagtgactta
      841 cttaggctat caaggttggg tgcatctaca gcaaggaaga tatatgtggt caaaatgggg
      901 tgtgaccatt ttcgtctatg acgacattat cttccttggc gatttggaat gatagtgtgc
      961 caggtgccct ctctttcccg tgtggcagcc ttcaactcgt tgagggctgc ttctcgatgt
     1021 gacacaggca ttcgtgcccg tgatcttctg ttctgtgaca cagacaagag tgtctgtttc
     1081 acactgacct gggttcgcag ggcgtctgtt cgggaatctg tttgtggtgc aggcatcttg
     1141 cctgcgaaat gcttctc
//
LOCUS       DAOQUC010001516         1156 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_180040_length_1156_cov_4.237057, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001516 DAOQUC010000000
VERSION     DAOQUC010001516.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1156
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_180040_length_1156_cov_4.237057"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1156
                     /locus_tag="PKH07_19550"
     CDS             <1..>1156
                     /locus_tag="PKH07_19550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37196.1"
                     /translation="GDALNGKIYELLANNVINNYGLQDRYVAFGSPNAIYSTTKMDWA
                     LFGRAATATLAPSTMALIGKIDWTSEIRRDLEEKYGKQLSQEYINRLATDFPFLAMNL
                     FAGKTDIDGVNDFMSKFNSVTYEQHEIGLQMYDERLKWANLVATGKQMGLTDDECYAV
                     LQMVRDSYSDQDRAKFDEWMSSGSFENDMKIAATLLDPLGGIAPLTRDPELQSSVMAG
                     TMIAGALAGFAMGGPAGAVMGGLMGVFAGSEPFNLFNTNVFAQRELDKAKNFGIADPA
                     SDLKAFEDRSRDLQQLYKAHLERGDLDAAHKIALSGVELTNQIAEWALENKYALQQAG
                     VLNMIRQDIANSHERWEASTIGDSLERDFVPRKLELPPEFTPGKDIMTLDG"
BASE COUNT          346 a          269 c          339 g          202 t
ORIGIN      
        1 gggagatgcc ctgaacggga agatctatga gctgctagcg aacaacgtga taaacaacta
       61 cgggctacag gacagatacg ttgcgtttgg aagcccgaac gcgatctact ccaccacgaa
      121 gatggattgg gcattgtttg gacgagcagc gacagcgacg ctagcaccaa gcacaatggc
      181 cctaataggg aaaatagact ggacatcaga gatccgaagg gatttggaag agaaatacgg
      241 gaagcagctg tcacaggagt acatcaacag gttggcaacg gatttcccat tcctggcaat
      301 gaacctgttt gcagggaaaa cggacatcga cggagtcaac gatttcatgt caaagttcaa
      361 cagcgtaaca tatgaacaac atgagatcgg attgcagatg tacgacgaaa gactgaagtg
      421 ggccaatctg gttgcaaccg ggaaacagat gggactcact gatgacgaat gctacgccgt
      481 gttgcagatg gtgcgggatt catactcaga tcaggatagg gcgaaattcg atgagtggat
      541 gtcgtcagga agcttcgaaa acgatatgaa gattgcagcg acgctgctag acccgctagg
      601 agggatcgcc ccactcacga gagaccctga gctgcagtca agcgtaatgg ccggcacgat
      661 gatcgccgga gcgttggcag ggtttgcaat gggaggtcca gcaggcgcag taatgggtgg
      721 actgatggga gtgtttgccg gaagcgagcc gttcaacctt ttcaatacaa acgtgttcgc
      781 tcaacgagaa ctcgataaag caaagaactt cgggatcgca gacccggcaa gcgacctgaa
      841 agcgtttgag gaccggagcc gagacctaca acagctgtat aaggctcacc tggaacgtgg
      901 cgatctggat gccgcgcaca agatcgcatt gagcggagtc gaactgacca accagatagc
      961 agaatgggca ctggaaaaca agtacgcact acaacaagca ggcgtactga acatgatccg
     1021 acaggacata gccaactcac acgagaggtg ggaggcatca accatcggag acagtctgga
     1081 acgagacttc gtgccccgga aactggaact accaccagag ttcacgccag gaaaggacat
     1141 catgaccctg gatggt
//
LOCUS       DAOQUC010001517         1156 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_180060_length_1156_cov_3.552225, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001517 DAOQUC010000000
VERSION     DAOQUC010001517.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1156
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_180060_length_1156_cov_3.552225"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..693
                     /locus_tag="PKH07_19555"
     CDS             <1..693
                     /locus_tag="PKH07_19555"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNT37197.1"
                     /translation="SYWIHREVDAYFVACERSRRELALRHVPLEKIFVTGIPVNPDFA
                     HREDTRQIRTRLGLDENRKTVLMMGGGLGLVAYDKLVRSILKTESNAQIVSIVGADGK
                     RLDSLRSRLPSERVTVLGYVNNVHDWMNASDVIVSKPGGLTCAEVLARGLPFVMTSPL
                     PGQEEVNVHYMLSSGAAVYAESPRQVAEWVNTLLNSENAKEYLTSKALAFGRPNAVRD
                     IVTLAVQNVGGA"
     gene            943..>1156
                     /locus_tag="PKH07_19560"
     CDS             943..>1156
                     /locus_tag="PKH07_19560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37198.1"
                     /translation="MKQEKAVKKKAGKSVSEAKSGFCTDFPVSEFILRNYKNFNARVT
                     RDALLAYWQHIERGGKMFWAVAGAMSS"
BASE COUNT          259 a          285 c          328 g          284 t
ORIGIN      
        1 tcatactgga ttcatcgcga agtggatgct tactttgtcg cctgtgagcg cagcagacgc
       61 gaacttgccc tgcgtcacgt cccgctggag aaaatcttcg tgacggggat ccccgtcaac
      121 ccggactttg ctcatcgcga agatacgagg cagatccgaa ccagactcgg cttggatgag
      181 aaccgcaaga ctgtgcttat gatgggggga gggcttgggt tggttgccta tgacaaactg
      241 gtgcgttcga tccttaagac cgaaagcaac gctcagattg tctccattgt gggagctgat
      301 gggaaacgtc ttgacagcct tcgttctcgc cttccatcgg aacgcgtcac agtgcttggc
      361 tacgtcaaca acgtccatga ctggatgaat gcatccgatg tcattgtcag caagccgggt
      421 ggcttgacgt gtgccgaagt gcttgcacga ggactgccct ttgtcatgac gagtcccctg
      481 ccgggtcagg aagaggtcaa tgttcattac atgctaagca gtggtgctgc ggtctatgcc
      541 gaatcgccaa ggcaggttgc cgaatgggtc aatacactcc tgaattcaga gaacgcaaag
      601 gaatatctta cctccaaggc tttggctttc ggtcgtccca acgccgtgcg cgatatcgtt
      661 actcttgccg ttcaaaacgt gggtggtgcg taatgtggga tttcgttcag ggctcttcga
      721 ttgcgtacgg catgggtgcc acggtcatat cacctcagat catacacatg ccgtctccaa
      781 aacaacacgc ctgggattgc ggagtgtccg ctcggcagac gtcctctccc gccgagatga
      841 agcagcgtca tcatgatgtc tgatgacaat gattgttgac ccattccatg aatctggcat
      901 acagtcacac gttattgtta ctcgattgag tgaggttttc tgatgaagca agagaaggca
      961 gttaagaaaa aggcgggcaa gtctgtgtct gaggccaaat ctggtttctg caccgatttt
     1021 cctgtcagcg agttcattct tcggaactac aagaatttca atgcacgagt gacgcgagat
     1081 gccttgttgg cgtactggca gcatatcgaa cgagggggaa agatgttctg ggctgtggcg
     1141 ggagcgatgt cctccg
//
LOCUS       DAOQUC010001518         1155 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_180331_length_1155_cov_3.324545, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001518 DAOQUC010000000
VERSION     DAOQUC010001518.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1155
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_180331_length_1155_cov_3.324545"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..380
                     /locus_tag="PKH07_19565"
     CDS             <1..380
                     /locus_tag="PKH07_19565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37199.1"
                     /translation="PVVTQQFIGTLNSAYSFLPNCLTTPVAHITKAAMTDVFTENRYD
                     DIHNRIFWQNCMWLHIHYNTAGWQDPLFSTTFFPAYGFWKTGSLGPDCDYDGGFNIYD
                     PTNGVISAGDIYRTQKRPLGSVD"
     gene            complement(606..>1155)
                     /locus_tag="PKH07_19570"
     CDS             complement(606..>1155)
                     /locus_tag="PKH07_19570"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNT37200.1"
                     /translation="DVADALIEKMKQNGAVVVTSSQTAQLEKVVLNEEGHPHRDYIGR
                     DASVILDAIGVKVPRETRIILAEVPQSHPFVQEELMMPLLPFVRVANFEEGLEKAKEA
                     EHGFRHTAMLHSRNMDRVTRFAREMNVTMLIVNGMCGNVLASQGEGYAAMTIAGTTGE
                     GITTPRSFCKMRRLTFKDQLNV"
BASE COUNT          278 a          340 c          275 g          262 t
ORIGIN      
        1 cccctgtcgt cacacagcag ttcatcggca ctttgaattc agcttattcc tttctgccca
       61 actgcctcac aacacctgtt gcccacataa caaaggcagc gatgactgat gtcttcactg
      121 agaaccgata cgatgacatc cacaaccgca ttttctggca gaactgcatg tggctgcaca
      181 tccactacaa cacggccgga tggcaggatc cacttttcag tacgactttc tttcccgcct
      241 atggtttctg gaagaccggc tcactcggac ctgactgtga ttacgacggg ggattcaaca
      301 tctacgaccc gaccaacggt gtcatcagtg cgggagatat ctacaggact cagaaaaggc
      361 ctctcggatc ggtggactga gctcagcaac ggcaccgatg caggacattt gcctgtccac
      421 attccgcctt ccacgacatc gcagaaaagg cacatcctct cgtgggtgca gaacgtagga
      481 agatgcgccc agactgagaa cagccgtatc tccgaaacac ggtcgtctgc aagatagtgg
      541 catctggggt gatacaccgt cactcttttc cccaagacca actaagcgaa cgtcggtggc
      601 atgcttcaga cgttcagttg gtccttgaag gtcaagcggc gcattttgca gaagctgcgc
      661 ggtgtggtga tgccttcgcc cgtagtgccg gcaatggtca tcgcggcgta tccctcaccc
      721 tgagaggcaa ggacattccc gcacatgccg ttgacaatga gcatcgtcac attcatctca
      781 cgagcaaatc gcgtaacgcg atccatattt cgcgaatgaa gcatcgccgt atggcgaaat
      841 ccatgctcgg cttccttagc tttttccaaa ccttcttcga agttggccac acgaacaaaa
      901 ggcaagagag gcatcatcaa ttcttcctga acaaaagggt gactctgcgg aacttcagcc
      961 aggatgatac gcgtttcccg aggcaccttg actccaatgg cgtccagaat caccgaggca
     1021 tctctgccga tatagtcacg atgcggatgc ccttcttcgt tgagaacaac cttttccaac
     1081 tgcgcggtct ggctcgatgt gacgacgacc gccccatttt gcttcatctt ctcaatgagc
     1141 gcatcggcaa catca
//
LOCUS       DAOQUC010001519         1155 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_180375_length_1155_cov_2.773636, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001519 DAOQUC010000000
VERSION     DAOQUC010001519.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1155
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_180375_length_1155_cov_2.773636"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..556
                     /locus_tag="PKH07_19575"
     CDS             <1..556
                     /locus_tag="PKH07_19575"
                     /inference="COORDINATES: protein motif:HMM:NF036518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycerol-3-phosphate ABC transporter ATP-binding
                     protein"
                     /protein_id="HNT37201.1"
                     /translation="QSTVIYVTHDQMEAMTLGNRIVVLKDGLIQQVGAPLEIYDHPIN
                     KFVGGFIGTPPMNFLEGKVVQRDGACYFDQGSFAIRLPDNFERVLDGSKERGVIFGIR
                     PEHLVDIAVVQEKDPESFITANVEVVEPIGAESLWYVKVGTSSLVARVEAHCRAKVNE
                     VKTLQVDVAKAHLFDSVTEQTLLV"
     gene            582..>1155
                     /locus_tag="PKH07_19580"
     CDS             582..>1155
                     /locus_tag="PKH07_19580"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HNT37202.1"
                     /translation="MPFRKKRQDIQEDERFVIEYDLGEGLKRVRTTVHARTDKVLKIL
                     APLRNGIPLPLPKGLKMRASVVRDDALYLFDSLIIDREAGNVPVLVIPVPDELERVQR
                     RQSYRLSTEIPVELIVPAGDTATARQRHSIDLSGTGLRIAGEPLPLGMIVHLKMHLPN
                     RNTPIFAVAEVVRVESSEVGEFSRCLALKFV"
BASE COUNT          264 a          271 c          321 g          299 t
ORIGIN      
        1 gcagtccacg gttatctatg tcacgcacga ccagatggaa gcgatgactc ttggaaacag
       61 aatagtggtt ctgaaagatg gcctgataca gcaggttggt gctcctctgg agatttacga
      121 tcatcccatc aacaagtttg ttggtggctt tatcgggacg cctccaatga actttctcga
      181 gggaaaggtt gttcagcgtg atggagcgtg ttatttcgat cagggctcct ttgcgattcg
      241 gctgcctgac aatttcgaga gagttctcga tggttccaaa gagcggggcg tgatttttgg
      301 gatcagacct gagcatctcg ttgatattgc tgttgtgcag gaaaaggatc cggaatcctt
      361 cattacggcg aatgtagagg ttgtcgagcc cattggggcg gaatcccttt ggtatgtgaa
      421 ggttggcaca tcgagcttgg tggctcgagt tgaagcgcat tgccgtgcca aggtcaatga
      481 ggtcaagacg cttcaggttg atgttgccaa agcgcacctc ttcgattctg tcacagagca
      541 gactttgctt gtgtaatctc ctgtgctgac aagagagttc catgccattt cgaaagaagc
      601 ggcaagatat ccaggaagat gaacgcttcg tcatcgagta tgatctgggt gaagggctca
      661 agagagttcg taccacagtt catgcgagga cggacaaggt tctgaaaatc ctggcacctc
      721 tgcgcaacgg aatccctctt cctcttccca agggactcaa gatgagggct tcagtagttc
      781 gggacgatgc cctatacctc ttcgattcat tgatcataga ccgcgaagcg gggaatgttc
      841 cagtcctggt tatcccggtt cctgacgaac tcgagcgtgt tcagagaaga cagtcctacc
      901 gcctttccac agaaatccct gtcgaactga tcgtccctgc cggtgacacg gcgacagctc
      961 gacagagaca ctcaatagat ctcagcggta cgggtcttcg aatcgctggc gagcccctcc
     1021 ctttgggaat gattgtccat ctcaagatgc atcttccaaa tcggaataca cccatttttg
     1081 cggtggcgga agttgttcga gtggagtctt ctgaagtcgg agagtttagc cgttgtctgg
     1141 ctctgaaatt cgtgg
//
LOCUS       DAOQUC010001520         1155 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_180496_length_1155_cov_1.930909, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001520 DAOQUC010000000
VERSION     DAOQUC010001520.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1155
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_180496_length_1155_cov_1.930909"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1012)
                     /locus_tag="PKH07_19585"
     CDS             complement(<1..1012)
                     /locus_tag="PKH07_19585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37203.1"
                     /translation="MMPDTKSLSDKGLSMATLSPVSPWLGASLIILFTLCAHYPVLLA
                     DYVWDDDGVIVNNEELRDIDGLWRIWMNPSSMEQEHHYWPLTYSTFWLEYQIWGARPL
                     GYHVNNLVLHTLNALLFWWLLARMRVVGNWFAAALFAVHPVHVESIAWVTERKGLLAG
                     LFFLLAFHCFVRFRREGLRRFYLATCVLFVLGLLSKSSVFALPLVLAVWLWFDNATDL
                     GKRLLGLVPIGFVGLGMGLADWWFFRDRYSVDLGFSLFQRLVIAGRVIWFYLSKIVVP
                     FPLLAVYPRWELQHGLLLQCLYPTALLLVAWILWWQRKRTGRAPLAAVASFVALLLPV
                     LGF"
BASE COUNT          358 a          328 c          287 g          182 t
ORIGIN      
        1 taaaacccaa gacaggcagc agcaaggcaa cgaaagaagc aactgctgcc aagggtgctc
       61 tacccgtccg ctttctctgc caccagagaa tccatgcgac gagtagtagg gctgtgggat
      121 acaggcactg caggagaaga ccgtgctgga gttcccatcg cgggtagaca gcaagcagtg
      181 gaaagggcac aactatcttg ctgagataaa accagatcac tcgcccggca atgaccaacc
      241 gctgaaagag gctgaaaccg agatcaacac tgtaacgatc tcgaaagaac caccagtctg
      301 caagtcccat ccccaacccg acaaaaccga tgggaacaag cccgagaagt cgctttccaa
      361 ggtcagtcgc attgtcaaac cacagccaga ccgccagaac gaggggcaat gcaaacacgg
      421 aagacttgct cagcaggcca agcacgaaca atacacaagt cgcaaggtag aaacgcctca
      481 acccctccct tcggaagcgc acaaagcagt gaaaagcaag aaggaagaac agaccggcaa
      541 gaagtccctt gcgttctgtc acccaagcga ttgattcaac gtgtaccgga tgcaccgcaa
      601 agagcgccgc cgcaaaccag tttcccacaa ctctcatcct cgccaggagc caccagaaaa
      661 gcagcgcgtt aagagtgtgc aggacaagat tgttgacatg atatcccaga ggtcgcgctc
      721 cccagatctg atactccagc cagaatgtcg agtaagtcaa tggccaatag tggtgctcct
      781 gctccatgct cgaaggattc atccagatac gccagagacc atcaatatcc cgcaactcct
      841 cgttgttgac aatgacacca tcgtcatccc aaacataatc ggcaagaagc actggatagt
      901 gtgcgcagag agtgaacagt ataatcagcg aagccccaag ccacggagaa acgggagaaa
      961 gagtcgccat ggacagtccc ttgtcagaaa gtgacttggt atcgggcatc atcaacatcc
     1021 ttgctgcata caggcaaagg gctcctgtct caagcagagt cttgcatgca agataggtca
     1081 gatgccgtcg gcatgcaaag cagaaaagcg acgaggcgtc cgaccagagc cagacgcctc
     1141 acagcacaat cgtaa
//
LOCUS       DAOQUC010001521         1150 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_181674_length_1150_cov_2.919635, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001521 DAOQUC010000000
VERSION     DAOQUC010001521.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1150
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_181674_length_1150_cov_2.919635"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..893)
                     /locus_tag="PKH07_19590"
     CDS             complement(<1..893)
                     /locus_tag="PKH07_19590"
                     /inference="COORDINATES: protein motif:HMM:NF015604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046559 - alpha-glucuronidase activity
                     [Evidence IEA];
                     GO_process: GO:0045493 - xylan catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucuronidase family glycosyl hydrolase"
                     /protein_id="HNT37204.1"
                     /translation="MATGSVTADQLMIFEGGKAHVSIVCPSSRSLTEDSAVQQLRYFL
                     QEMTGSEFVVADHPHGPCIVVGEHSSLRGLLPAKKAMNLVPESFNILIHNGSVFIVGA
                     DDRGVLYGVYRFLEKLGCRWLAPAFTYYGDDHSFVPRVKSLVLNLDASCVEKPALKYR
                     KLYVEEGHSHTAENLLQLIDWMPKRRFNTLVVPLDYGGRGRVRWDKWRDALTVELKKR
                     GIIIEVGGHGYENFLNAKMEEGKIFETHPEWFGMNEEGLREAKQNRVFCTSNDGARRY
                     VLGKLLEYLEAHPEIEIFDFWP"
     gene            complement(868..1017)
                     /locus_tag="PKH07_19595"
     CDS             complement(868..1017)
                     /locus_tag="PKH07_19595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37205.1"
                     /translation="MTIIVLVGYYSVPEESGDSLAVEQVDWNVSETWIHHRGCYVHGY
                     WICHG"
BASE COUNT          312 a          332 c          252 g          254 t
ORIGIN      
        1 ggccagaaat cgaatatctc tatctcagga tgcgcctcca gatactccaa gagtttgccc
       61 agaacatacc gtctcgcacc atcgttggat gtgcagaaaa cacggttctg cttcgcctcc
      121 ctcagacctt cttcgttcat gccaaaccat tcaggatgcg tttcaaatat cttgccttcc
      181 tccatcttgg cattgaggaa gttctcataa ccatgaccac ccacctcgat gatgatgccc
      241 cgcttcttca gctcgacggt cagtgcgtct cgccacttgt cccaccgaac gcgtccacga
      301 cctccatagt ccaatggaac gacaagagta ttgaagcgcc ttttcggcat ccaatcgatc
      361 aactgaagaa gattctccgc tgtgtgagag tgcccttctt caacatagag ctttcggtat
      421 ttgagagcag gtttctcgac acaggacgca tccagattga gaaccagaga tttcaccctg
      481 ggcacaaatg aatgatcgtc tccatagtag gtgaacgccg gagccagcca gcgacagcca
      541 agcttttcaa gaaatctgta gacaccgtag agcacaccac gatcatcggc accaacgata
      601 aaaacactcc cgttgtgaat caatatgttg aacgactccg gcacaagatt catggccttc
      661 tttgcaggaa gcagacctct cagggaactg tgctctccca caacaatgca gggaccatga
      721 gggtgatccg ccacaacgaa ctcggaaccg gtcatctcct gcaggaaata cctcagctgc
      781 tgaactgcag aatcctcagt caaagaacgg ctgctcgggc agacaatgct gacatgagcc
      841 ttgcctcctt caaagatcat caactgatca gccgtgacag atccagtagc catgtacata
      901 acacccacga tgatgaatcc aagtctccga aacattccaa tcaacctgct ccacagcaag
      961 tgaatcacca ctctcctcag gtacgctgta atagccaacc aacacgatga tagtcatgca
     1021 atcggtctgc tgtcaaagcc gcagaggtgc gagcaccctt gacagtttga gtgtgctggg
     1081 gctagtatcc gagagtgatt acagaggcaa caacaggaga aggcatcatc tcgctattgt
     1141 ctatggtcgc
//
LOCUS       DAOQUC010001522         1149 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_181917_length_1149_cov_3.675503, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001522 DAOQUC010000000
VERSION     DAOQUC010001522.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1149
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_181917_length_1149_cov_3.675503"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(399..>1149)
                     /locus_tag="PKH07_19600"
     CDS             complement(399..>1149)
                     /locus_tag="PKH07_19600"
                     /inference="COORDINATES: protein motif:HMM:NF019368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HNT37206.1"
                     /translation="HRLRALFDSGFHPKNSVASGAFDVLTRNVALPDTSTWTEPITGT
                     NPHYGLVAVENGKRGVAVLSHGLTEYEVFDTDSGTIALTLLRAFSYPKMSGLALENRV
                     ARVGNDNSQCFGKHAYALALCFYSGSWDKSGIVDQMQQHQYPPMTVHHSPYKGEGLSR
                     CESFFRLEPSVLSLSAIKQSEDGTMTIVRFFNPLDEPVQGHFWSRTAVKSVALLNLDE
                     TIASKGTVTRKHHIALTVPAKKIVTLGLTFE"
BASE COUNT          257 a          306 c          335 g          251 t
ORIGIN      
        1 tggattcaag ataaatcagc agccctcaga accgcttcgc gaacctgaag gctggtatcc
       61 agagaagtca gtcggagcat gagaagaggg agggcacccg gcaactcctt ctcctgcggg
      121 agaaggtcgg gatgagggtg tcggagagat tccttgctga agactcgctg gaaacaagtt
      181 cctcacaaaa cctggtgtct ttgtgtcttg gtggtgaatc ttcttctccg agtgtgggga
      241 gcgttttgcc cgtggggaac aggagtccac gaccgagacg gtcgtgtggc gactgcagaa
      301 cctcatccac agcgtttctg ccgactcaca gggaaatagc cttagacggc ttgatggcac
      361 acctggctgc gcgctgtgcg gatcggaatc aagaggcgct actcaaacgt caggcccagt
      421 gtaacaatct tcttcgcggg tacggtcagc gcaatatggt gttttcgagt caccgttccc
      481 ttgctggcaa tcgtttcatc gagattcagc aatgcgacgc tcttgaccgc ggttctcgac
      541 caaaagtgtc cttgcaccgg ctcgtcgagt gggttgaaga aacgcacgat agtcatggtg
      601 ccgtcttcgc tttgtttgat tgcggagaga ctgagcaccg acggttcgag acggaaaaat
      661 gactcacagc gcgagagtcc ttcgcccttg taggggctgt gatgcaccgt catgggcgga
      721 tattgatgct gctgcatctg atccacaatg cccgacttgt cccatgagcc ggagtagaaa
      781 cacagcgcga gagcgtatgc gtgtttgcca aagcactggg agttgtcatt gccgactcgc
      841 gccacgcgat tctccagcgc caagccggac atcttcggat agctgaatgc ccgaagcagc
      901 gtcagcgcaa tagttcccga atccgtgtcg aagacttcat attccgtcaa gccgtgtgaa
      961 agcaccgcaa caccgcgctt tccgttctcc acagcaacca gaccgtagtg aggattggtt
     1021 cccgtgatcg gttccgtcca ggtagatgtg tcggggagcg caacgtttcg ggtgagcaca
     1081 tcaaatgctc ctgaggcaac cgagttcttc gggtgaaagc ccgaatcaaa caacgcccga
     1141 agcctgtga
//
LOCUS       DAOQUC010001523         1149 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_181920_length_1149_cov_3.618830, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001523 DAOQUC010000000
VERSION     DAOQUC010001523.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1149
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_181920_length_1149_cov_3.618830"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1149)
                     /locus_tag="PKH07_19605"
     CDS             complement(<1..>1149)
                     /locus_tag="PKH07_19605"
                     /inference="COORDINATES: protein motif:HMM:NF019298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HNT37207.1"
                     /translation="TLLFEPALITDAKGQATLAIDMADSITTWRMTAMASSKAGALGS
                     TEHPIRVFQDFFVDIDLPVALTKNDRVSIPVVLYNYLESSQKVRIKFDVEPWFELDGD
                     AEQVIELEKEEVRSMYFPIQVKELGKHRLTVWAYGEKMSDAIRREIEVLPDGEETNVT
                     FSDRLSSEVQQTIVIPTEAIDGASKILVRVYPGVYSQIVDGLDSMLQMPFGCFEQTSS
                     TTYPNILIVQYMKATKQINPETQMKAEGFINAGYQRLLSYEVSGGGFSWFGDAPANKV
                     LTAFGLKQFHDMSKVHEVDPAVIERTRAWLLSKQESNGAWKPDENYLHAESWSGIQKS
                     DVLVTAYIVDAILSSEGTGAGVDKAIDYLRANWKDAGEAYVVSLVANAL"
BASE COUNT          247 a          332 c          292 g          278 t
ORIGIN      
        1 cagggcattg gccaccagag agacgacata agcctctcca gcgtccttcc agttggcgcg
       61 aagataatca atcgctttgt ccactcctgc gcccgtgcct tcgctgctga gaatcgcgtc
      121 aacgatgtag gctgtcacaa gcacatcgct cttctgaata cccgaccagg actctgcgtg
      181 aaggtagttc tcatcaggct tccatgcacc attggattcc tgtttggaga gaagccatgc
      241 tcgcgttcgt tcgatgacag cggggtccac ttcatgcacc ttcgacatat catggaactg
      301 cttgagtcca aacgctgtca aaaccttgtt tgcgggcgcg tcaccaaacc atgagaaccc
      361 gccaccactg acctcatacg acagaagcct ctgatagccc gcgttgatga atccttcggc
      421 tttcatctgt gtttctgggt taatctgctt ggttgccttc atgtattgca cgatcagaat
      481 gttggggtag gtcgtcgaac tggtctgctc aaagcacccg aagggcatct ggagcatgct
      541 gtccaaaccg tcaacgatct ggctgtacac accggggtag acgcgaacca ggatcttgct
      601 tgcaccgtca atggcttcgg tcgggatgac tatggtctgt tgcacttcac tggaaagtcg
      661 gtcgctgaaa gtgacattgg tctcctcgcc gtccggcagc acttcgatct cccgacgtat
      721 cgcgtcactc atcttctcac cgtaagccca gacggtcagg cgatgcttgc cgagttcctt
      781 gacctggatc gggaaataca tggaacgaac ttcttccttc tccagttcga tgacctgctc
      841 ggcatctcca tccagctcaa accaaggctc gacgtcaaac ttgatgcgga ctttctgtga
      901 gctttcgaga tagttgtaaa gcaccacggg gatcgaaacg cggtcattct ttgtcaatgc
      961 cacaggcaga tcaatgtcca caaagaagtc ctgaaatacg cggatcggat gctccgtgct
     1021 tccaagggct ccagccttcg aggacgccat cgcggtcatt cgccacgtcg tgatgctgtc
     1081 cgccatgtca atggcaagcg ttgcctgtcc cttggcatcc gtgatgagtg ccggctcgaa
     1141 cagaagcgt
//
LOCUS       DAOQUC010001524         1149 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_181937_length_1149_cov_3.233090, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001524 DAOQUC010000000
VERSION     DAOQUC010001524.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1149
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_181937_length_1149_cov_3.233090"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="PKH07_19610"
     CDS             <1..276
                     /locus_tag="PKH07_19610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37208.1"
                     /translation="DTNSDGSTDHFVTVIGYGEDGTSQYYGFLDTWDTQIHWQKFQAI
                     ASGRPWGIWGGTRFAIQDVSTPTPTNTPLATPTPLHTGMSPTLWDFD"
     gene            307..>1149
                     /locus_tag="PKH07_19615"
     CDS             307..>1149
                     /locus_tag="PKH07_19615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37209.1"
                     /translation="MARIAICLLSLVILPFACYANPSVVNFNAEGIAMVDGKPFFPIG
                     IFTYDLNSHVMADIHEQRFNTILHGFEPQQLDYIHQHGLMAVCYSDDKWFDAARNHPA
                     LLAWYLTDEPESRGFTPESERQRYLELKEKDPNHPIGLCHFLFEALDKFKNGCDFTMT
                     DVYPITWDRDVPIVNVGIHIDQARAVHHPNWPNWTYIQVFGGQETDGGKWAQPLPHEV
                     RCMAFMALVHRSTGILYFSYWPKAPQTWASIGQVNRDIYRILPWLLASGTEVPAQCTD
                     EPVQV"
BASE COUNT          275 a          315 c          278 g          281 t
ORIGIN      
        1 gacacaaact cggacggctc tacggatcat ttcgtgacag tcattggcta cggagaggat
       61 gggaccagtc aatactacgg attcctggac acatgggaca cccagattca ctggcagaag
      121 tttcaggcca tcgcttcagg caggccttgg ggcatctggg gaggaacgcg atttgccatt
      181 caggatgtct ctactccaac gcctacgaat actccccttg ccacacccac cccactacac
      241 actggcatga gtccaacttt gtgggacttt gactagataa tccgatctca gaaaggtctt
      301 gactccatgg cgagaatagc aatatgcctt ctgtcgttgg tcattcttcc ctttgcctgt
      361 tatgcaaatc cctctgtcgt caatttcaat gcggagggta ttgccatggt tgacggcaaa
      421 cccttctttc ccattgggat attcacctac gatctgaaca gccatgtgat ggcggacatc
      481 cacgagcagc gattcaacac aattctacac ggatttgagc ctcagcaact tgactatatc
      541 catcagcacg gtttgatggc tgtttgctac agcgacgaca aatggttcga cgccgctcgg
      601 aatcacccag cactgctggc ctggtacctg acagatgaac ccgagagccg aggtttcaca
      661 cccgaatccg aaaggcagcg ttatctggaa ctgaaggaga aggatcccaa tcatcctatc
      721 ggactttgtc actttctctt cgaagcattg gacaagttca agaacggttg cgatttcact
      781 atgaccgatg tctatccaat cacttgggat cgcgatgtcc ccatcgtcaa cgtaggtatc
      841 cacattgacc aagcccgtgc tgtacatcac ccgaactggc ctaattggac ttacattcag
      901 gtctttggtg gtcaggaaac cgacggaggc aagtgggcac agcccttgcc gcacgaagtg
      961 cgttgtatgg cgttcatggc attggtacat cgttcgacgg gaatcctcta tttctcctat
     1021 tggccgaaag cgccccagac ttgggcttcc atcggacaag tcaaccggga tatctatcgg
     1081 attttgcctt ggctgctggc atcgggcact gaagtacctg cccaatgcac agacgagcca
     1141 gttcaagtt
//
LOCUS       DAOQUC010001525         1149 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_182054_length_1149_cov_2.077697, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001525 DAOQUC010000000
VERSION     DAOQUC010001525.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1149
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_182054_length_1149_cov_2.077697"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(290..601)
                     /locus_tag="PKH07_19620"
     CDS             complement(290..601)
                     /locus_tag="PKH07_19620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005993614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HNT37210.1"
                     /translation="MVKKLYVGNLPFSATEDEIRDLFSEHGTVHSVRLITDRETGRPR
                     GFGFVEMDGDAAEAAASALNGQDMGGRALKVNEARDREGGGGGDSRGRDRDRRPPRRD
                     Y"
     gene            complement(866..>1149)
                     /locus_tag="PKH07_19625"
     CDS             complement(866..>1149)
                     /locus_tag="PKH07_19625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HNT37211.1"
                     /translation="EAIRLSAGLLKSSYVDYEFRTTIHPSLIHPDDILQIAREIRGAK
                     AYIVQPFYLTTCLDPTLCIPPDGFVTEFEEALQSVQSYFGRVQIRGTQA"
BASE COUNT          274 a          324 c          305 g          246 t
ORIGIN      
        1 cctgaatatg gacttcaggc gaatgcagtt tcccagttga gcacagacac ctccacgagc
       61 aggcatgagc cttagaccga caaacacagg cccactctcc agagcagatc tgcacctgca
      121 ggtgtgcggg ctatgcccac tcccttggat gcttgctcgg caaatgacac gcttcttctg
      181 gactgagtgt cattgggaag attgacttca caaagacccg atctctttgc agaaaaggtc
      241 acctgcaaaa gcaggcgtaa cccttcttac gccgaagagc gcaggacgct cagtaatctc
      301 ggcgtggggg tctgcgatct cggtctcgtc cgcggctgtc tccgccaccg ccaccttcgc
      361 gatcgcgcgc ttcgttgacc ttcagggcac gaccacccat gtcttggcca ttgagagcgg
      421 atgcggcggc ttccgcagcg tcaccatcca tctcaacgaa gccaaagcca cgcggacgac
      481 cggtttcccg gtctgtgatc agccggactg agtggactgt tccgtgttcc gaaaacaggt
      541 cgcgaatctc atcctcagta gcactgaacg gcaggttccc aacgtacaac ttcttcacca
      601 tctcgactcg actcccaaaa gtaacaatag tctccacctg tccttcagtg atacactgac
      661 tgtacaaagt gggccaaatg tggtgcttta gcagggcttg atgcccttct agcaccgctc
      721 ggaactatta ccagaagccc ccatcttagt caaatgaaat aatcacgtct gtggtagcaa
      781 agggaaaatg tcccctgtgg aaaacggaga attgccgcag acgacggcgg aggaacgcaa
      841 gagatgaagt tggttggcca gatggtcagg cttgtgtgcc gcggatctga actcgcccga
      901 agtatgactg aaccgactgc agggcttctt cgaattcagt gacaaatccg tcaggcggga
      961 tacagagagt tggatccagg caggtagtca gatagaaggg ttgaacgatg taggctttcg
     1021 cccctcgaat ctcccgcgca atttgaagga tgtcatcggg atgaatcaga ctcggatgaa
     1081 tcgtcgtgcg gaattcatag tccacgtaac tgctcttgag cagtccggcg ctcaggcgaa
     1141 tcgcttcca
//
LOCUS       DAOQUC010001526         1146 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_182703_length_1146_cov_4.120073, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001526 DAOQUC010000000
VERSION     DAOQUC010001526.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1146
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_182703_length_1146_cov_4.120073"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..729
                     /locus_tag="PKH07_19630"
     CDS             <1..729
                     /locus_tag="PKH07_19630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37212.1"
                     /translation="RALALRRLQRVGTLCPKRPRTPCSEDLTSGHAQRALYLDSYCER
                     YFLVRANSENLLYIEIMSQFTDAAGQIYETEEDATKQVHTERAEQDQGFSGASLFFTG
                     VISFVLGGIVVAYAAKQTVARGTDFRYLADRLASDPRMPDAKFKKVVRDPEFGNLLRV
                     NGFDVVASSGPVFAWLDDVSDPPVIRAANLFTGQEYPKTFKTQAALETNFDTVVVPYL
                     DPEVSPFPEFSHSEEEELERHSST"
     gene            777..>1146
                     /locus_tag="PKH07_19635"
     CDS             777..>1146
                     /locus_tag="PKH07_19635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNT37213.1"
                     /translation="MQASVLPADTVGIFCQRHGLSREALFALNPHLNMRSFYIESGIL
                     TAVLDEGEQLRTSVGVGAAPAVTYCQTNEGQGGWQKMDGRWYYANHTGLTLSALANRY
                     GTDVMTIWKGSRSAGTIPTTW"
BASE COUNT          250 a          294 c          318 g          284 t
ORIGIN      
        1 cgggcgctgg cgcttcgacg gcttcagcga gttggtacct tgtgcccgaa gcgtccacga
       61 actccatgct ccgaggatct cacgtcaggc cacgcgcagc gagcgcttta tttggatagc
      121 tattgtgaga gatactttct tgttcgtgcg aactctgaaa atctcttgta catagagatc
      181 atgtcccaat tcaccgatgc tgctggccaa atctacgaga ccgaagaaga tgctacgaaa
      241 caagttcaca cagaacgtgc cgaacaggac caggggttct ctggggcttc tctgttcttc
      301 actggagtga tttcttttgt tttgggagga attgtggtgg cttatgcagc aaagcaaacg
      361 gttgctcgtg ggacggattt tcgatacttg gcagaccgat tggcctctga tccgcgtatg
      421 cctgatgcca agttcaagaa agtggttcgt gaccccgaat tcgggaactt gttgcgagtc
      481 aacggcttcg atgtcgttgc ttcgagtggc cccgtgtttg cgtggcttga tgatgtgtcg
      541 gatccgcctg tcatccgtgc tgccaacctt tttactggtc aagagtaccc aaaaacgttc
      601 aaaacccaag cggcgcttga aactaatttt gacacggtgg tggtacccta cttggatcct
      661 gaagtgagtc cttttccaga gttttcccat agcgaagaag aagaattaga gagacactcc
      721 tcgacctaaa gagaagtcgc tggcgcgctt cttggtgctt ggggtaccat tggctcatgc
      781 aagcatccgt cctccctgcc gatacggtcg gcattttctg ccagcgccac ggcctctcgc
      841 gagaggcgtt gtttgcgctc aatccacatc tgaacatgcg atccttctac attgaaagcg
      901 gcattctcac agccgttttg gatgaaggcg agcaacttcg tacgtcggtc ggagtcggcg
      961 ctgcaccggc tgtcacttat tgccagacga acgaaggtca gggtggttgg caaaagatgg
     1021 atggccgctg gtactacgcg aaccatacgg gattgaccct cagcgccctg gcaaatcgct
     1081 atggcacgga cgtgatgacg atctggaagg gatccaggtc ggctggaacc attccaacga
     1141 catggc
//
LOCUS       DAOQUC010001527         1145 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_182978_length_1145_cov_4.867890, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001527 DAOQUC010000000
VERSION     DAOQUC010001527.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1145
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_182978_length_1145_cov_4.867890"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            189..602
                     /locus_tag="PKH07_19640"
     CDS             189..602
                     /locus_tag="PKH07_19640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013044007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNT37214.1"
                     /translation="MGRFRDLSADDGNETGIDISPLMDCVFILLIFFIVTTTFVDETG
                     VEVDKPQAASSVRLEKTSILIALTDKGEVVYGGREIGISGVQPLVKRMLQKEEVPVII
                     QADAAVQSGLLIRVLDEAKLAGATKVSVATRNRAA"
     gene            617..>1145
                     /locus_tag="PKH07_19645"
     CDS             617..>1145
                     /locus_tag="PKH07_19645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37215.1"
                     /translation="MRTRRRESFWIVRRLFHHLLVIVGSVGLTLVFFLVLPLMQTLTK
                     PLTPDLLVQSVDTGKLEAPEPPPEEPVEKQPEPEEEPPKLIEDTQPLDLSQLEMALNP
                     GVNEGLLAGDFVVKLNSVVSESTDMDALFSLSDLDQKPRVIYQPAPVLTAELRKKAPG
                     TVYLVFVVDQQGRVEE"
BASE COUNT          270 a          278 c          328 g          269 t
ORIGIN      
        1 gcgctgataa cgacagaaac aggcctggta atcgccctgc ctggactgtt cttccagtat
       61 ctgctggttc gcaaactgga gcgatacaag gcgtttttga cgcatttgga gactgtgtgt
      121 acgcagaagt tctacaagag gcttcgcaga gataagaagc aatagccaat cctaaatagg
      181 aggagatcat gggacgtttt cgagatttgt ctgccgatga tggcaatgag acgggtattg
      241 acatatcgcc gctgatggat tgtgtgttca tcctgctcat ctttttcatt gtcacaacca
      301 cttttgttga tgagacaggt gtcgaagtgg ataagccaca ggctgcctct tcggttcgcc
      361 tggagaagac cagcattctc atcgcattga ccgacaaagg tgaggtggtt tacgggggtc
      421 gcgagatcgg tatcagtggc gtacagcctc tggtgaagag aatgctgcag aaggaagagg
      481 ttcccgtcat cattcaggca gatgcggcgg tgcagtcggg tcttctgatc cgagttcttg
      541 atgaagccaa acttgcggga gccaccaagg tcagtgtggc gacgagaaat cgcgcggcat
      601 gacaacgcag gattagatga ggacacgcag acgagaatca ttctggatcg tccgacgact
      661 gttccaccat ctgctggtca ttgtgggctc tgtcggtttg acgcttgtct ttttcctggt
      721 cttgccgctg atgcagacgc tgaccaagcc cttgacaccg gacttgttgg tgcagtcggt
      781 ggacacagga aagctcgagg cgccggaacc tccacccgaa gaacccgtgg agaagcaacc
      841 cgaaccggaa gaggaaccgc ccaaattgat cgaggatacc cagcctttgg acttgtcaca
      901 gttggaaatg gctttgaatc caggtgtcaa tgaaggtctg cttgctggtg actttgtggt
      961 gaaactcaac agcgttgtct ctgaaagcac ggacatggac gccttgttct ccctctccga
     1021 tctcgaccag aagccacggg tcatctacca gcctgccccg gtgttgaccg ctgaactcag
     1081 aaagaaagct cctgggacag tctacctggt ctttgttgtc gaccagcaag gtagagtcga
     1141 agaac
//
LOCUS       DAOQUC010001528         1145 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_183060_length_1145_cov_2.672477, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001528 DAOQUC010000000
VERSION     DAOQUC010001528.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1145
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_183060_length_1145_cov_2.672477"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..427
                     /locus_tag="PKH07_19650"
     CDS             <1..427
                     /locus_tag="PKH07_19650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37216.1"
                     /translation="AKMRVIDHLGDCSSRLIKSNDLYGDCGTTIGFLFDSERQARQFI
                     KLTAERALHCSTPADSGRHIYSNWEVVMNRRGAHSPLLNPFTLPENRGSKVKYTAGMC
                     PQTLDVLKRTVFLGVSPSWTATDTKRIAQICKQISESVT"
     gene            502..>1145
                     /locus_tag="PKH07_19655"
     CDS             502..>1145
                     /locus_tag="PKH07_19655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37217.1"
                     /translation="MANRILNALCIFSFLVLMATPCSAQTKDFPFKGSESLPPRALST
                     SGPTRPAELNTAEPASLKTLLSSTVISDVSAYLWWDGCGPTAVGMVVGYYDTCGYPDL
                     VPGDAMTDLPTVRQMIASHGSPSNPQHYEDYSEPIDSYGNIKSDKSELPTGDEHTSNC
                     LADFMHTSWSREGLPYGWGYSDMISVGWLQYVNLVTSNYQATAENYWPSKPITWS"
BASE COUNT          276 a          319 c          275 g          275 t
ORIGIN      
        1 tgccaagatg cgagttatcg atcatcttgg ggattgctcc tcgcgcctga tcaagtctaa
       61 tgatctgtac ggagactgcg gaacaacaat cggctttctg tttgattccg aaaggcaggc
      121 cagacagttc atcaaactga cggcagaacg tgcgctccat tgttcaacac ctgctgactc
      181 aggcaggcac atctactcca actgggaagt tgtcatgaat cggcgaggcg cacacagtcc
      241 tctccttaat cccttcaccc ttccggagaa tcgaggttcc aaggtcaagt acaccgcagg
      301 tatgtgtcct caaacgctcg atgtgctcaa aagaactgta ttccttggcg tctccccatc
      361 atggactgcc accgatacaa agagaatcgc ccagatatgc aaacagatca gtgaatcggt
      421 aacataggtt gccacagaag cccgggaagg ctatatttat gttttgatgt tgtgagtttc
      481 cttgatgaag ggatgcgact gatggctaac agaatcctga acgcactttg catcttcagt
      541 ttcctggtgc tcatggcaac accctgctcc gcacaaacca aggactttcc ttttaaggga
      601 tctgagagtc ttccgcctcg cgccctcagc acatccggcc cgactcgtcc tgccgagttg
      661 aacactgccg agcctgcttc cctaaaaacc cttctttcga gcacggtcat ctcagacgtg
      721 tctgcatatc tctggtggga tgggtgtggc ccaacagccg tcggaatggt ggttggatat
      781 tacgacacct gtggctatcc cgatctcgtc cctggtgacg caatgacaga tctccccacc
      841 gtacgccaga tgattgcctc tcatggttcg ccgtcgaatc cacagcacta cgaggactat
      901 agcgagccca ttgacagtta tggcaacatc aagtcggaca aatcggaact gccgacaggc
      961 gatgagcaca ccagcaactg tcttgcagat ttcatgcaca cttcgtggag ccgtgaaggt
     1021 cttccctacg gttggggcta ttcagatatg atctcggtgg gttggcttca atatgtgaac
     1081 cttgtcacct ccaactacca ggcaacagca gaaaactatt ggccttcaaa accgatcacg
     1141 tggtc
//
LOCUS       DAOQUC010001529         1144 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_183320_length_1144_cov_3.132231, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001529 DAOQUC010000000
VERSION     DAOQUC010001529.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1144
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_183320_length_1144_cov_3.132231"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1144)
                     /locus_tag="PKH07_19660"
     CDS             complement(<1..>1144)
                     /locus_tag="PKH07_19660"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNT37218.1"
                     /translation="RTLSDDRGRFSLDDLEQGKAQIRSVTQGFMTTRLADVTTGSENV
                     VIVLEKGGSLTGSVRLENGEVVSGATVELTPKTALDDWAKRGNLQVITDEKGSHLLQG
                     LEPGTYDAVASTTEMFSDVQKEIEILAGKETPLDFVLKKGVRISGRVLAETHADDQPV
                     AGANLKCQLSHYGMTRSATSSAIGEFEFERVPSGLATVNLNAPSGYIPKSDSRQVVVG
                     DEDVTDLEFRVLLGGKISGIVLGPEGSGISGARVEIKKSGFLGMGFNHPESVSDATGF
                     YLIDGLEEGQDYVLHGSATGYAPAKSEPFALKKGEERHDFNLVFALGGSISGKVTDAS
                     GEPLEGIAVRAGSTIFEMDRVGAGISKTNELGFYRIEHVPEGQVTVM"
BASE COUNT          267 a          361 c          260 g          256 t
ORIGIN      
        1 catcaccgtg acctggcctt cgggaacatg ctcgatgcga tagaagccca gttcattggt
       61 cttggaaatg ccggcaccta cgcgatccat ttcaaaaatc gtgctgccgg cgcgaacagc
      121 gatgccttcc agaggctctc ccgaagcatc tgtcaccttg ccggaaatcg agccgccaag
      181 cgcaaagaca agattgaagt cgtgcctttc ctcacccttc ttcagtgcaa agggttcact
      241 cttggcgggt gcatatcctg tcgcggaccc atggagcaca tagtcttgtc cctcttccaa
      301 accatcaata aggtagaagc cggtcgcatc ggacaccgat tccggatgat tgaaacccat
      361 tccgagaaaa ccagacttct tgatctcaac tcgggctccc gagattccag acccttccgg
      421 acccagaaca attccggaaa tcttgccccc caacagaacg cgaaactcaa gatcggtgac
      481 atcctcatca ccgaccacca cctgccggga atcagatttc ggaatatatc ccgatggtgc
      541 gttcagattc accgtagcca gaccggacgg cacacgctca aactcaaact ctccgatcgc
      601 ggatgatgtg gcacttcgcg tcattccgta atggctcaac tgacatttca gattggcacc
      661 tgccacaggc tgatcgtctg catgcgtttc agccagcaca cgacctgaaa tacgaacacc
      721 cttcttgaga acaaaatcga gtggtgtttc cttccccgca agaatctcga tttccttctg
      781 aacgtcactg aacatttcgg tcgtgctggc aactgcgtca taagttcccg gctcgagtcc
      841 ctgcagaaga tgggaacctt tttcatccgt gataacttgc aggtttcctc gttttgccca
      901 gtcgtccaaa gccgtctttg gtgtcagctc gacggtagcg cccgatacca cttccccatt
      961 ctcaaggcgc acagagcccg tcagcgaccc acccttctca agaacgataa caacgttctc
     1021 ggagcccgtc gtcacgtccg ccaaccgggt tgtcatgaag ccttgcgtca cagatcgaat
     1081 ctgtgccttc ccctgctcga ggtcgtcaag agagaagcgt cctcgatcat cggacagcgt
     1141 cctc
//
LOCUS       DAOQUC010001530         1143 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_183619_length_1143_cov_2.970588, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001530 DAOQUC010000000
VERSION     DAOQUC010001530.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1143
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_183619_length_1143_cov_2.970588"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1143
                     /locus_tag="PKH07_19665"
     CDS             <1..>1143
                     /locus_tag="PKH07_19665"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HNT37219.1"
                     /translation="ELGPGLLGNIYDGVQRPLEALRNASGHFIQRGTQAPSLDRERRW
                     HFVPRADKGQAVSKGTLLGVIPETHLIEHRVLVPPDMTGTLLSVAVEGDYRVEDTIAE
                     VQTQAGVQKISLIQRWPVRVSRPSAGRLRPDTPLVSGQRVIDTLFPIAKGGAVAIPGG
                     FGTGKTMTQHAIAKWCDAEVIVYIGCGERGNEMTDVLMEFPKLQDPRSGRPLMERTIL
                     LANTSNMPVAAREASIYTGITLAEYYRDMGYDTAIMADSTSRWAEALRELSGRMEEMP
                     AEEGFPAYLPTRLAEFYERAGRFETLSGQKGSITIIGAVSPPGGDLSEPVTQHTKRFI
                     RGFWALDRELANARHYPAISWMDSYSEYVDEVTHWWEENVASDWHEN"
BASE COUNT          250 a          290 c          347 g          256 t
ORIGIN      
        1 gaactaggcc ccggactttt gggcaacatc tatgacggtg tgcaaagacc tttggaagcg
       61 ctcagaaatg cgagtggtca cttcattcag cgtggcactc aggctccttc cctggaccgt
      121 gagaggcgct ggcattttgt tcctcgtgca gacaaagggc aagccgtctc caaaggaacg
      181 ctccttggag tcataccgga gacgcatctg atcgagcacc gagtcttggt gcctcctgac
      241 atgacgggaa cgctgttgag cgttgctgtc gagggagact accgggttga ggacacgatc
      301 gcggaagttc aaacgcaggc gggcgtgcaa aagatcagcc tcatccagcg gtggcctgtt
      361 cgcgtcagca gaccctctgc ggggcggctt cgtcccgaca ctcctttggt ctccgggcag
      421 agagtcattg acacgttgtt cccaattgcc aagggtggtg cggttgctat tcccggtggt
      481 tttggaaccg gcaagacgat gacccaacat gccattgcca aatggtgtga tgctgaagtc
      541 atcgtttata tcggctgtgg tgaacgtggc aacgagatga cagatgttct gatggaattc
      601 cccaagttgc aggatcctcg ttccggtcgt ccgctgatgg agcgaacgat tctgctggca
      661 aatacctcga atatgcctgt tgccgcccgt gaagccagta tctacacagg catcactctt
      721 gcagagtact acagggacat gggctatgac accgcgatca tggcagattc gacttcgcgt
      781 tgggctgagg cgttgcgaga gctctcaggt cgaatggaag agatgcctgc cgaggaaggc
      841 tttcctgcct atctgcccac gaggttggcg gagttctatg agcgcgcggg cagatttgag
      901 actctttcgg ggcagaaggg atccatcacg atcattggtg ctgtttcacc cccaggtgga
      961 gatttgtcag agccggttac acagcacacc aagcgcttca tcagaggctt ctgggctttg
     1021 gatcgggaac tggcaaatgc acgacactat ccggcaatca gttggatgga cagctacagt
     1081 gaatacgtgg atgaagtcac ccattggtgg gaagagaacg ttgcgtcgga ctggcatgag
     1141 aac
//
LOCUS       DAOQUC010001531         1142 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_183933_length_1142_cov_2.628335, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001531 DAOQUC010000000
VERSION     DAOQUC010001531.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1142
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_183933_length_1142_cov_2.628335"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            252..635
                     /locus_tag="PKH07_19670"
     CDS             252..635
                     /locus_tag="PKH07_19670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37220.1"
                     /translation="MELSPLELERVPILSGADIVTYTWETHEIELTEPARERVARLEV
                     PMTTGVPFVVCVGDERIYGGAFWVSSSSMSFQGIVIDTLFAKMDGHPIRIQLGYPESP
                     ERFEGEDLRSDPRILRSLTGSGKLW"
     gene            complement(618..>1142)
                     /locus_tag="PKH07_19675"
     CDS             complement(618..>1142)
                     /locus_tag="PKH07_19675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37221.1"
                     /translation="TAHFRTFLTSSARAPLAWRKRRVNGNFLTSELSTLPIAGLAQHH
                     RALQAEIKWCPIRPKWCPVLCPTVAEWCPICRLFVTPRVQSALVHCTHAVASLVGLAV
                     GGVLERRCCPRLVQTLTGKVPQTTMTSARSAQLHGQRRPLVGRYSKDNLRAVGQNIRA
                     QTAKNELASSPQLT"
BASE COUNT          236 a          309 c          347 g          250 t
ORIGIN      
        1 aggggcggct gatcgtgatc gtgggcgatc atccggacgt gcagcgtgcc ctggaggcca
       61 ttctggggcc gcagttcgcg ggagggtagg agcacatgaa atttaggcac ctggtgtcac
      121 gaaccactag tctgtggatt gtcatagccg tcacctccgg ttgcgcaggc gcagcgtcag
      181 agccagatac aatgggagac ttctctatct atttggtcaa acaaggcatc tctgctcagc
      241 agatgatgga gatggaactg tccccgctcg agcttgagag agttccgatt ctgtctggcg
      301 ctgacatcgt cacgtatacc tgggaaacac acgagattga gctgacagaa cctgctcgtg
      361 agagagttgc acgcctcgaa gtcccgatga cgaccggagt gccctttgtg gtctgtgtcg
      421 gagatgaacg catatacggt ggggcattct gggtttcctc gtcatccatg agcttccagg
      481 gtattgtgat cgataccctc ttcgccaaga tggacggcca tcccatccga atacagcttg
      541 ggtaccctga atctccagag cgtttcgagg gagaagacct acgatctgat cccaggatac
      601 tgcggtcact tacgggttca ggtaagttgt ggtgaacttg ccagttcgtt cttggcggtt
      661 tgggcgcgga tgttctgccc aacagctctt aagttgtcct tggagtaccg cccaacaagc
      721 ggccggcgct ggccatgcaa ctgcgccgac ctggcggagg tcatcgttgt ttggggcacc
      781 ttgccagtca acgtttggac aagccttggg cagcacctcc gctcaagaac tccgcccacc
      841 gccagtccga ccagtgacgc caccgcatgt gtgcagtgta ccagcgctga ctggacacgt
      901 ggtgtcacaa agagacggca aatgggacac cattctgcca cggtgggaca caaaacggga
      961 caccattttg gccgaatcgg acaccatttg atctcggctt gtagtgcgcg atggtgctgg
     1021 gccagacccg ctattgggag tgtgctgagt tccgaagtca gaaagttccc gttcacgcgc
     1081 ctctttcgcc aggccagcgg cgcgcgcgca gatgatgtca gaaaggtgcg gaaatgggcc
     1141 gt
//
LOCUS       DAOQUC010001532         1141 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_184246_length_1141_cov_2.302947, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001532 DAOQUC010000000
VERSION     DAOQUC010001532.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1141
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_184246_length_1141_cov_2.302947"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..188
                     /gene="nadA"
                     /locus_tag="PKH07_19680"
     CDS             <1..188
                     /gene="nadA"
                     /locus_tag="PKH07_19680"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006558712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HNT37222.1"
                     /translation="SRVIFFLIPTDWLLCPNMKKMTLESIRNALETMQPVVRIESDIA
                     DRARLALTRMLQVVGGS"
     gene            complement(281..>1141)
                     /locus_tag="PKH07_19685"
     CDS             complement(281..>1141)
                     /locus_tag="PKH07_19685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37223.1"
                     /translation="DDILAEFVRRGVRLGYVDQNLLEIVDLTSEGGLIRAPVNWQDAK
                     RVQIVAPLFLGLGFLLLRTTALFRFPRRMLLSGWRLFRLVGVRNRQARKELYRLDEMS
                     LLVSKDKLVLLADGFKSAIATSSANLFKLNRVVHIVHQMKEDQGILNALDEREQEGTL
                     HSRGRMHRERLQRRLALYRAKHADIAHLFSDTSNPTLHQIRSALAWVKQQSRPLRKWF
                     WSASPNQLRWLQGILGQWVDSASQDVVWRRSVANSAWAILAVSTAGCWFLRSLSLPWI
                     YIVVRWINGA"
BASE COUNT          272 a          304 c          297 g          268 t
ORIGIN      
        1 tttccagagt catcttcttc ctgattccaa ccgattggct cctgtgcccg aatatgaaga
       61 agatgactct ggaaagcatc cgaaatgcac tggagacgat gcaaccggtt gttcgcattg
      121 agtcagacat tgccgatcgt gcccgactcg ctttgacccg aatgcttcag gtagtgggtg
      181 gatcgtagac aggtctttcg gtggctcggg tcaccatgct tctcggattc tccaactttc
      241 gcgtgattgc gttggcgtcg ttcagtcttc cctggcgggt ctatgctccg ttgatccatc
      301 gcacaacgat gtagatccac ggcagagaga gcgaccgtag aaaccagcaa cctgccgtgg
      361 acactgcaag aatcgcccat gctgaattcg ccacagatct tcgccagacg acatcctgtg
      421 acgcagaatc tacccactga ccaaggatgc cctgaagcca tctcagttga tttggactgg
      481 cactccagaa ccatttgcgc agaggtcggg attgttgttt cacccatgcc aatgcggaac
      541 gaatctggtg cagtgttgga tttgaagtgt ccgagaaaag atgtgcaatg tcggcatgtt
      601 ttgctctgta caaagccaga cgacgctgga gacgttctcg atgcatcctg cctcgggaat
      661 ggagcgttcc ttcttgctcg cgttcatcga gggcgttgag aatcccttgg tcttctttca
      721 tttgatggac aatatgaaca actcggttca gtttaaacag gtttgccgat gaagtcgcga
      781 tagcagactt gaagccatct gccaagagaa cgagtttgtc cttgctgacc agcagggaca
      841 tctcatccag ccggtagagt tctttgcggg cttgacgatt ccgcacacca acaagtcgga
      901 acagtctcca accactcaag agcatccgcc tcggaaaacg aaagagggcg gttgttctga
      961 gcaagagaaa accgagtcca aggaaaagtg gggcaacgat ttggacgcgc ttggcatcct
     1021 gccagttcac aggcgctcgg atcagcccac cttcactggt gagatccaca atctcaagaa
     1081 ggttctgatc aacatatccc agtcggacgc cacggcgcac aaactcagcc agaatgtcgt
     1141 c
//
LOCUS       DAOQUC010001533         1140 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_184415_length_1140_cov_3.639631, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001533 DAOQUC010000000
VERSION     DAOQUC010001533.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1140
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_184415_length_1140_cov_3.639631"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1140
                     /locus_tag="PKH07_19690"
     CDS             <1..>1140
                     /locus_tag="PKH07_19690"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HNT37224.1"
                     /translation="GSDTTRSRVLVLGMDGLEWDVINELMAEGKLPNFTRLIEQGTSG
                     KFKSIRPILSPVIWTSIATGKSPSKHEITFFTVDNPKTGEQHPITSDFRKTKAVWNIL
                     SDNDRSVGVIGWWATWPAERVNGFMISDHVNYHFLHAKESEKTVITGNTYPPELYQKI
                     HEHLAKPMEVDAKDVREFIHLEDFPSDGVFSFSDPVDHFRWAYATSESHRILGLNALK
                     EQTLDALMIYFEGPDSVSHLFGQYYRPDPERAKTLDAQKLEQFGRVVEGFYVYMDSII
                     GQFLEIMPQDMNLIVCSDHGFDLKEGTGVASGGAMELKEISERAHKEYGVLFLFGPDF
                     KMGHQIMGAHVYDIAPTVLHLLGLPIPEDMEGRLLSEAFRNQVRQV"
BASE COUNT          280 a          279 c          307 g          274 t
ORIGIN      
        1 ggcagcgata ccacccgcag tcgtgtcctc gtgctgggca tggacggtct ggagtgggat
       61 gtcatcaatg agttgatggc agagggaaag ttgccgaact tcacacggct gattgagcag
      121 ggcaccagtg gcaaattcaa gagtatcagg ccgattctct ctcctgtcat ctggacgtct
      181 atcgctacgg gaaagtctcc atccaaacac gagatcacct tcttcacagt ggacaatccc
      241 aagaccggtg agcagcatcc aattacttct gactttcgaa aaacgaaagc agtctggaac
      301 atcctttcgg acaacgatcg gagtgtcggt gttatcggtt ggtgggcaac ctggcctgct
      361 gagcgggtca acggcttcat gatcagcgac cacgtcaact atcattttct ccatgccaaa
      421 gagtcggaaa agaccgtgat aacaggcaat acgtatcctc ccgaactcta tcagaagatt
      481 cacgagcatc tcgccaaacc gatggaagtt gacgccaagg atgttcggga gttcatccat
      541 cttgaggact ttcccagtga tggggtattt agtttctccg atccggttga tcacttccgc
      601 tgggcttatg ccacatcaga gagccatcga atcctagggc tgaacgctct gaaggagcaa
      661 acgctcgatg ccctgatgat ctactttgaa ggtcccgaca gtgtctcgca cctttttggt
      721 cagtactacc gacctgatcc agaaagggcg aaaacgctgg atgcgcagaa actcgaacag
      781 tttggacgag tggtggaggg attctatgtt tatatggaca gcatcatcgg tcagttcctg
      841 gagataatgc ctcaggatat gaacctgatt gtctgttcgg atcacggttt tgatctcaaa
      901 gaaggcaccg gtgtggcaag tggtggcgcg atggaactga aagagatcag cgagcgtgcc
      961 cacaaggaat atggtgtgct gttcttgttc ggtcccgatt tcaagatggg tcatcagatc
     1021 atgggagctc atgtctatga tattgctccg accgtgcttc acctgctcgg cctgccgatt
     1081 cccgaagata tggaaggcag actgctctca gaagcctttc ggaatcaagt acgtcaagtg
//
LOCUS       DAOQUC010001534         1140 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_184420_length_1140_cov_3.496774, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001534 DAOQUC010000000
VERSION     DAOQUC010001534.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1140
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_184420_length_1140_cov_3.496774"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            145..>1140
                     /locus_tag="PKH07_19695"
     CDS             145..>1140
                     /locus_tag="PKH07_19695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37225.1"
                     /translation="MKNTNTRVIVTILLSVCCHGAFAATPLERCGYQTLRIVMEQLPN
                     TSDWLSWYNENNDAMWVYNQHMDIYGYVAADGSWGQNYKNEFGGWPSDTELHNLWGYN
                     WGDAVAMTVWVWESQCGRIKETDIIFNPSYTWTTDPLAAEGNSSVLYYTSILYHEMGH
                     SWGLQTRNETYDYDVPTVMHYYKSSMVQDVHTIHTPDAKMLRENYSNQTSAPDRLDMG
                     VHSKNAQGTWQNASTNKRNYIQGEPLSVYGMLVENVGNKGASNVRLRFYLSENRTISV
                     SDYLIDEWSWGSFAKATWSVFNFENMTIPSDIPAGNYYVGALISVDGYSQDDLYDN"
BASE COUNT          296 a          304 c          284 g          256 t
ORIGIN      
        1 gcccgaacgt gttgcaggtt ccccttcccc aaattccagg gtttccatca tggaaccgaa
       61 tctggcgaag tcgcaacaac ccttgacact gaaacaactg ctcaaaaccg ttgaagcgct
      121 tgccaccacg aaaggagaac aacagtgaaa aacacaaaca cccgggtcat cgtcacaatt
      181 ctcctttcgg tctgttgcca cggggcattt gcggcgaccc cgcttgaacg ctgtggctac
      241 cagacgctgc gaattgtcat ggaacagctg cccaacacat cggactggct ttcctggtac
      301 aacgagaaca atgacgccat gtgggtctac aaccaacaca tggacatcta tggatatgtg
      361 gccgcggatg gttcatgggg acagaactac aagaatgagt ttggtggctg gccgtcggac
      421 acggaactgc acaacctttg gggttacaac tggggagatg ctgtcgcaat gacggtttgg
      481 gtctgggagt cgcagtgcgg ccgaatcaag gaaacggata tcatcttcaa tcccagttac
      541 acttggacaa ccgatccact cgcagcagaa ggcaattcct ccgtgctgta ctacacatcc
      601 atcctttatc acgagatggg acactcttgg ggacttcaga ctcgcaatga gacttacgac
      661 tacgatgttc caacggtgat gcactactac aagtcctcca tggttcagga tgtgcacacc
      721 atccatacgc cggatgccaa gatgcttcgg gagaactatt ccaaccagac gtctgctcca
      781 gacagactcg acatgggagt tcactccaag aatgctcaag gtacctggca gaacgcctcc
      841 acgaacaagc gcaactacat tcagggtgag ccgttgtctg tctacggaat gttggtcgag
      901 aacgttggga acaaaggcgc atcgaatgta cggcttcggt tctatctttc ggagaaccgc
      961 accatatcag tttcagacta tctgatagat gaatggtcat ggggttcctt tgccaaagcc
     1021 acatggagcg ttttcaactt tgagaatatg acgattccct cggacatacc cgctgggaat
     1081 tactacgtgg gtgctctcat cagcgttgat ggctattccc aggatgatct ctacgacaat
//
LOCUS       DAOQUC010001535         1140 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_184431_length_1140_cov_3.348387, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001535 DAOQUC010000000
VERSION     DAOQUC010001535.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1140
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_184431_length_1140_cov_3.348387"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            715..>1140
                     /locus_tag="PKH07_19700"
     CDS             715..>1140
                     /locus_tag="PKH07_19700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat pathway signal protein"
                     /protein_id="HNT37226.1"
                     /translation="MPKQSENPSMIWAYLLHLGYNMWSDRENPGVGDGHFGCRPYLRC
                     DKSLWDDVLKKMVDSGVNMVVIDLGEGVRYDSHPEIGIRNAWSPAKLKRELVKMRKMG
                     LEPIPKLNFSTCHDTWLGTYHRMISTPKYYEVCKNLIAEV"
BASE COUNT          282 a          314 c          283 g          261 t
ORIGIN      
        1 tatagccagt ctcccgcata aggacaagat cacttgagca gacaaagcaa tgacctcgtg
       61 caagaatatg gtaatagacg ctccaaccta cagaaggacc taggatgatg cccgactccc
      121 gaggctgttc tacctcaaag ggaccgctgt agaaaaacgc ccttttgact ctcggacgcc
      181 cttgggacgt ccgaggtggg tccggcggga gtgcccagta ttcttcagct tggcgcacaa
      241 caagtatgaa acaatcttcc ttttcaaacg tgcgatccga aaggcttttg atctcgacct
      301 caaaggcaag gggaccccga atctcgacga acggaacaca cgaaccagcc caagtagata
      361 ggaggcatga cacaagaaca gccaaccttt ctgatttccg catggtcctg tgccgggtgc
      421 cctctttctt ctcatgctcc gactgacttc tctgggtgcc agcccttcag gttcgcgaag
      481 cggttctgag ggctgctgcc ttatcatgga tccatgaact caccacagtg ccaagtctct
      541 catatatgag gtatacggtc aaatccttca ggaggtgcgt ctcgcccaca cagatttcgt
      601 cgggagcaag atgctcccgc tcctctgacc cacccacact tcccgcttgc gtcccttttt
      661 ggtttcgact accgtttgca gtattgactt gttcccgaaa aggagaaaag caccatgcca
      721 aaacaatctg agaatccatc aatgatctgg gcgtatttgt tgcatctggg ttacaatatg
      781 tggtctgacc gtgagaatcc gggtgtgggt gacggacatt tcggatgccg accttatctg
      841 cgttgcgaca agagtctctg ggatgacgtc ctcaagaaga tggtggactc cggcgtcaac
      901 atggtagtca tcgaccttgg cgaaggcgtt cgatacgaca gccatcccga gattggaatt
      961 cgcaacgctt ggtcgcccgc caaactcaag cgggaacttg tcaagatgcg caagatgggt
     1021 ttggaaccaa tccccaaact aaacttctcg acctgccatg atacctggct gggaacctat
     1081 caccgaatga tctcgacgcc gaaatactat gaggtctgca agaatctcat tgcggaagtc
//
LOCUS       DAOQUC010001536         1140 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_184463_length_1140_cov_2.872811, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001536 DAOQUC010000000
VERSION     DAOQUC010001536.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1140
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_184463_length_1140_cov_2.872811"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(4..540)
                     /locus_tag="PKH07_19705"
     CDS             complement(4..540)
                     /locus_tag="PKH07_19705"
                     /inference="COORDINATES: protein motif:HMM:NF014614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HNT37227.1"
                     /translation="MSSSLVQVYFGDGKGKTTAAVGQAVRAAGQGMRVCFVQFMKDVS
                     FPIGERNSLKSLSRNIRVIVHEYPEDLYVDFRVTDLERFAEHVNHFLETVELIARSGQ
                     FDLLVLDELGAVLKLGLVSEARVLDLLKSRSSELDVVLTGRNFPQEIPDFADLVTEFK
                     KIKHPFDRGLPARKGIEY"
     gene            complement(656..>1140)
                     /locus_tag="PKH07_19710"
     CDS             complement(656..>1140)
                     /locus_tag="PKH07_19710"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HNT37228.1"
                     /translation="YKKTFGKFAKAAEDNGVKIAIENCPHGRKNLGFSPPMWEKMFNE
                     VPSPAIGLEFDPSHLVWMFIDWLPALYQFGDRVYMFHAKDTEIRHEVLSREGITAPGW
                     WRYRLPGWGQVDWKKVISALHDIGYKGDMSIEHEDPVYGGALTDKGLRMGLSHLRSLI
                     "
BASE COUNT          266 a          333 c          287 g          254 t
ORIGIN      
        1 cgcctagtac tcaatcccct tgcgtgcggg caaaccgcga tcgaacggat gtttgatctt
       61 tttgaactcc gtgacaagat cggcgaagtc gggaatctcc tgtgggaaat tccgccccgt
      121 cagcaccaca tcgagttccg acgaccgtga cttcagtagg tcaagaaccc gcgcctcaga
      181 aaccagaccc aacttcagca ctgcacccag ctcatcgaga accagaagat cgaactgacc
      241 gcttcgggcg attaactcca ctgtttccag aaagtgattc acatgctcgg caaagcgctc
      301 cagatcggtc actctgaaat ccacgtaaag gtcttctggg tactcatgga cgatcacgcg
      361 gatgttgcga ctgaggctct tgagcgaatt gcgttctccg atgggaaagg aaacatcctt
      421 catgaactga acaaagcaga cgcgcattcc ctgcccggcg gcacgaactg cctggccgac
      481 ggctgccgtc gtctttcctt tgccgtcccc aaagtatacc tgtaccaagc tcgatgacat
      541 gggcttgttc ctttcaacga tgcatgtttc tctcagtacc agagcgtttg gcagaggctg
      601 tcttacggtc tttctcggcg caaagccgag atgcagactg agacggacac agcactcaga
      661 tcaaggagcg cagatgcgac agccccatcc tgagtccctt gtcggtcaag gctccgccgt
      721 agaccgggtc ttcatgttca atggacatat cgcccttgta gccgatgtca tgcagtgcgg
      781 agatgacttt cttccagtcc acctgtcccc agcccgggag ccgatagcgc caccaaccgg
      841 gagcggtgat cccttcccga gagagcactt catgacgaat ctcggtatcc tttgcatgaa
      901 acatgtagac acgatctccg aactgataga gtgccggcaa ccagtctatg aacatccaga
      961 ccagatgaga aggatcgaat tccagcccaa tggcaggtga tggaacctcg ttgaacattt
     1021 tctcccacat cggtggagag aagccaagat tcttgcgtcc gtggggacag ttctcgatag
     1081 cgatcttcac cccattgtcc tcggcggctt ttgcgaactt gccaaaggtc ttcttgtagt
//
LOCUS       DAOQUC010001537         1139 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_184755_length_1139_cov_2.606089, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001537 DAOQUC010000000
VERSION     DAOQUC010001537.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1139
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_184755_length_1139_cov_2.606089"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(25..663)
                     /locus_tag="PKH07_19715"
     CDS             complement(25..663)
                     /locus_tag="PKH07_19715"
                     /inference="COORDINATES: protein motif:HMM:NF025840.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DarT ssDNA thymidine ADP-ribosyltransferase
                     family protein"
                     /protein_id="HNT37229.1"
                     /translation="MSEKRLTNHADFPRLMKPQWREFRSAVERHQISALYHCTPGANL
                     ETIFRVGGLLSRSEQLRLGIRPAKYHGWGKKWTSLADYVCLGFVSPRHIRDESDSNVV
                     LRISPEIIWLDGVVFCPCNSAKEWYSPEEIGARDDLDAFEYLFRNEHSMRLKRQDAEI
                     LVPKAVALDWVNAILFLDDKLLRAVRWRCLRSAPRRFFTRPSLWRRFMVGAH"
     gene            complement(685..>1139)
                     /locus_tag="PKH07_19720"
     CDS             complement(685..>1139)
                     /locus_tag="PKH07_19720"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar nucleotide-binding protein"
                     /protein_id="HNT37230.1"
                     /translation="QGMIGWLKGCLDKGEPVTLFTDEYRTPIYLPDLIAIVDEALRKD
                     LQGVYHVASHPRISRYDLGVLFAEVFGYDRNLIVPGSLKDYEGAKRPPDVSLNTRKLL
                     DKLDYRPLGVEEAIKHLKEEFDGKPSDSSLQKRRSDLYWGGPDRLPER"
BASE COUNT          282 a          313 c          289 g          255 t
ORIGIN      
        1 catagaaaga ggccgaaaca tggctcaatg tgctcctacc ataaatcttc gccagagaga
       61 aggcctcgtg aaaaagcgac ggggtgcact tctcaagcac cgccatcgca ccgctcggag
      121 taacttgtcg tcgagaaaga gtatggcgtt cacccaatcc aaggcaacgg ctttgggaac
      181 aagtatctcc gcatcctgtc gcttgagacg catactatgt tcattccgga atagatactc
      241 aaacgcatcc aagtcatctc gggcaccaat ctcttcaggt gaataccact ccttagcact
      301 gttgcaggga cagaaaacga ctccatccaa ccagatgatc tccgggctga tccgcagaac
      361 cacgttgctg tcgctctcat cccgaatatg gcgcggggac acaaagccca gacaaacgta
      421 gtcggcaagg gaagtccact tctttcccca tccatgatat ttggccggac gaatgccgag
      481 acgtaactgc tcggaacggc tcagaaggcc tccaactcgg aagattgtct ctagatttgc
      541 gccaggcgta caatggtaca gtgcggagat ctggtgtcgt tcaaccgcag atcggaattc
      601 acgccattgt ggcttcatga gccgggggaa gtccgcatga tttgtcagcc tcttttcact
      661 catggaatca gtcggttgct gagactacct ttcgggcaga cggtcaggtc ctccccagta
      721 gagatcggaa cgccgtttct gaagagaaga gtctgaaggc tttccgtcga actcctcctt
      781 cagatgcttg atggcctcct cgacgccaag cggacggtag tcaagtttgt caagaagttt
      841 tctggtattg agtgagacgt ccggaggacg ttttgcgccc tcatagtcct tgagagagcc
      901 gggcacaatg agattcctgt catagccgaa tacctctgca aacaaaacgc caaggtcata
      961 gcggctgatt cgaggatggc ttgccacgtg atacacaccc tgcaaatcct ttcgcagtgc
     1021 ctcatccaca atcgcgatga gatccggaag gtagatcgga gtacgatact cgtccgtgaa
     1081 gagtgtgact ggttccccct tgtcaagaca tcccttgagc cagccgatca tcccttgat
//
LOCUS       DAOQUC010001538         1138 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_184946_length_1138_cov_3.834718, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001538 DAOQUC010000000
VERSION     DAOQUC010001538.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1138
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_184946_length_1138_cov_3.834718"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(158..>1138)
                     /locus_tag="PKH07_19725"
     CDS             complement(158..>1138)
                     /locus_tag="PKH07_19725"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNT37231.1"
                     /translation="GYYLATQVLTTRRRILLLLVACLLSSGLLAVYGIATQAVQGSFR
                     AYQEIASPFFRNHCIYAAFLALSLSVAIAFALGCCHAGRRGLLYAFIGLFAGAIMLSF
                     VRGAWLSLGGMLLFNAWVFRRRIDYRVVTAIFLAGILLVVVLVGVGVGTKILERLGTI
                     ADTGYSANFDRLDRWAAAWRMYQDHPLAGVGYGTYADRRKEGYIHYRSAYSSRIRMGA
                     HNLYLEILAETGILGLFAFLWLMWRYFVGAARSLRHDSDPFRVSLIVGSVGAVITFLT
                     HAFVNNLGPSDKMFITFWLLIALVPITSRLRGTEEASRASSPAVPEVSGP"
BASE COUNT          306 a          318 c          297 g          217 t
ORIGIN      
        1 caggtcggat gtcgaggtgt ccatttgacc acgcgaccag attcaatcca ttgcgtgggt
       61 gtcaacagat gcaagtccac cccttcaacg cacgccaact ccatgcatct ctcatgtgct
      121 gaatccatca cacccgtctt gtcaacaata agagctttca tggtccagat acctcaggaa
      181 ccgcaggaga cgatgctcgt gacgcttcct cggtcccccg aagacgtgat gtgatcggaa
      241 cgagggcgat cagaagccag aatgtgatga acatcttatc agacgggccg aggttattga
      301 caaatgcgtg ggtgaggaac gtgatcacgg ctccgacaga tccgacaatg agagagaccc
      361 gaaatgggtc gctgtcgtga cgaaggctcc gcgctgctcc cacaaagtac cgccacatca
      421 accaaagaaa ggcgaacagt cccaagattc ctgtttccgc aaggatctcc agatagagat
      481 tgtgcgcgcc catccgaatt ctacttgaat acgcagacct atagtgaatg tagccttcct
      541 ttcttcggtc agcatacgtt ccatagccca ctccggccag agggtgatcc tggtacattc
      601 tccacgctgc agcccagcgg tcgagtcggt caaagttggc agagtatcct gtgtctgcaa
      661 ttgtgccgag tctctcaagt atcttcgtgc caacaccgac cccgaccaga acgacaacga
      721 gaagaattcc agcgaggaaa attgctgtga ccacccgata gtcaatgcgt cttcgaaata
      781 cccaggcatt aaagagcaac attcccccaa ggctcaacca ggctccccga acgaaagaga
      841 gcatgatcgc tccggcgaac agtccgatga acgcatagag aagtcccctt cgtccggcat
      901 ggcaacagcc caaagcgaag gcaatggcca cggagagcga cagagcaaga aaggcggcgt
      961 agatacagtg attcctgaag aagggagagg caatttcctg ataagctctg aaggatccct
     1021 ggacagcctg tgtggcaatg ccatagacgg ccagcagtcc actgctgagc aggcaggcga
     1081 cgagcagcaa gaggatacgt cttcgagtcg tcagcacttg agttgccagg tagtaacc
//
LOCUS       DAOQUC010001539         1137 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_185255_length_1137_cov_3.482440, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001539 DAOQUC010000000
VERSION     DAOQUC010001539.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1137)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1137)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1137
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_185255_length_1137_cov_3.482440"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="PKH07_19730"
     CDS             <1..271
                     /locus_tag="PKH07_19730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37232.1"
                     /translation="NSSDKYFVDRLWSAYLSRVPEREQVKVLSELLERNNIPPSDMKA
                     LFLPYLIHPVNERRDSEDQREPSDRDTPHHEGEVKAVNPCKSRLA"
     gene            318..866
                     /locus_tag="PKH07_19735"
     CDS             318..866
                     /locus_tag="PKH07_19735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012469521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNT37233.1"
                     /translation="MMRHKERSGFTMLELMVVVTILGILVAIAIPNFLACEVRAKVSS
                     VHASQGMLSIALETYFDDHNTYPLNSEKGALQGKDLSVLTRPIAYFTSLPTDPFDTSD
                     PRLRGRTLLGYLNYDQFPEALEGGEFLLEGQTLRPRYVLQSNGPDFEKDFSTGGKPWE
                     ILLYDPTNGTTSKGDVCRLGPQ"
     gene            888..>1137
                     /locus_tag="PKH07_19740"
     CDS             888..>1137
                     /locus_tag="PKH07_19740"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNT37234.1"
                     /translation="MRTRLGLQELIILTRQIGAALKMKLPLHGAFESESVQAPGRIRP
                     ILSDLARDLAKGERLSLALSRYPRYFPKYYRDAVDAGEK"
BASE COUNT          280 a          289 c          308 g          260 t
ORIGIN      
        1 gaattcctca gacaagtact ttgttgatcg cctctggtca gcctatctgt cccgagtacc
       61 tgaacgcgaa caggtgaagg tactcagtga gttgctggaa cggaacaaca tacctccctc
      121 tgacatgaag gctctctttc ttccgtacct gattcatcca gtcaatgaga ggcgagattc
      181 ggaggatcag cgtgagccgt ccgacagaga taccccgcac cacgagggag aagtgaaggc
      241 agtcaacccg tgcaagagcc gtctggctta aggaatagga agcaatgagt gtaggcaagt
      301 tctaaagaga ggaacaaatg atgagacaca aagaacgaag cggttttacc atgctggagt
      361 tgatggtagt ggtaacgatt ttggggatct tagttgccat tgcaatcccg aactttcttg
      421 cctgtgaggt gcgggcgaaa gtgtcttcag tacatgccag ccaggggatg ctgtccattg
      481 ccttggaaac atattttgac gaccacaaca cctatcccct gaattcagag aagggggcac
      541 tgcagggcaa ggacctgtct gttctcacaa ggccgattgc ctatttcact tcacttccga
      601 cagatccatt tgacacctca gacccgcgcc tcagagggag aacgcttctg ggttatctca
      661 actacgatca gtttcctgaa gcgttggaag gcggtgagtt tcttttggaa ggccagactc
      721 tgcgtcccag atatgtcttg cagagcaacg gtcctgactt tgagaaggac ttttccaccg
      781 gtggcaagcc ctgggagatt cttctctacg atccgaccaa tggaaccaca tccaagggcg
      841 atgtttgtcg cctggggccc cagtaagaga acaggaggca aacgcccatg agaacccgac
      901 ttggtttgca ggaattgatc atcctcaccc gacagatcgg tgcggcgctg aagatgaagt
      961 tgcctttgca cggtgccttt gaaagcgaat cggtgcaagc gcctggacgc atccgaccca
     1021 tcctttcgga tctggccagg gatctcgcaa agggcgagag actctctctg gcactttcaa
     1081 ggtatccccg atatttcccc aagtactacc gtgacgccgt tgatgctggt gagaagg
//
LOCUS       DAOQUC010001540         1137 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_185447_length_1137_cov_1.987061, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001540 DAOQUC010000000
VERSION     DAOQUC010001540.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1137)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1137)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1137
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_185447_length_1137_cov_1.987061"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..913
                     /locus_tag="PKH07_19745"
     CDS             <1..913
                     /locus_tag="PKH07_19745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014793190.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HNT37235.1"
                     /translation="ESPLYRSLNRYLQERFGQRVYKVTIDAGFTCPNRDGTIGTGGCI
                     YCNNQGFSPATALRSGSVGEQIASGVERLRRRYGATKFLAYFQPFTNTYAPVDELKSR
                     YEQALQFPEVVGMCIGTRPDCVADEVLSLLQSYAQHKEIWLEYGLQTIHDRTLALIQR
                     GHDFKTFLNTLERTRNRGIRICVHVILGLPGETLEMMMETIDAVAQMGVQGIKFHVLH
                     VLCETPLEQMYQEGNLLLLSEDAYVSLVSDSLERLPADMVIHRLASDAPREWLVAPEW
                     CLHKNQTISKIQQGLMSRGTGQGCRRQ"
BASE COUNT          266 a          296 c          318 g          257 t
ORIGIN      
        1 ggagtctcca ctctacagaa gtctcaatcg gtatcttcaa gagcggtttg gacagagagt
       61 ctataaggtc accattgatg cggggttcac ctgtccgaac cgcgatggaa ccatcggcac
      121 cggtggctgt atttactgca acaaccaggg attttctcct gcaactgcgc tgcgctcagg
      181 tagtgttgga gaacaaattg cgtctggtgt cgagcgactc aggcggcgtt acggtgcgac
      241 aaagtttctc gcctactttc agcctttcac caacacctat gcccctgttg acgaactgaa
      301 aagccgttac gaacaagcct tgcagttccc cgaagtcgtt ggaatgtgca ttgggacacg
      361 tcctgactgt gtggctgacg aggtgctgag tctgctgcag tcctacgcgc agcacaagga
      421 aatctggctc gaatacggac tgcagactat ccatgatcga acgcttgcgc tgatacagcg
      481 tggtcatgat ttcaagacct ttctgaacac ccttgaacga accaggaatc gaggcatccg
      541 gatatgtgtc catgtcatcc ttgggctgcc tggagaaact cttgagatga tgatggagac
      601 cattgacgct gtggcacaga tgggagtgca gggaatcaag tttcatgtgc tgcacgtcct
      661 gtgcgaaaca cccttggagc agatgtatca ggaaggaaat ctcctcttac ttagcgaaga
      721 tgcctatgtc tctctggtct ccgacagcct tgaacgatta cccgcggata tggtcatcca
      781 tcgtcttgct tccgacgcac cacgagagtg gcttgtcgcg cccgagtggt gcctgcacaa
      841 gaatcagacc atctcgaaga ttcagcaggg actcatgtct cgtggcacag ggcagggatg
      901 ccgacgccag tgattgacaa ctccttctcc tgcgggagaa gtccccagtg acacagagtc
      961 cgagaggggc gtatcatttg ttgagtaagc atccaataga gtgggcatag cccacaggcc
     1021 tgcaggtgct gacctgctcg ggatgagggt ctggcaatcc cccgccgaaa tcacgcgtca
     1081 tttggagccc gccaaaaaac tcaaaatagt tcttgacaaa gcggcggggg ggggggt
//
LOCUS       DAOQUC010001541         1136 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_185612_length_1136_cov_2.887142, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001541 DAOQUC010000000
VERSION     DAOQUC010001541.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1136
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_185612_length_1136_cov_2.887142"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="PKH07_19750"
     CDS             <1..294
                     /locus_tag="PKH07_19750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M24"
                     /protein_id="HNT37236.1"
                     /translation="EKGVEAYKAVGFPDEWKLHHQGGATGYEGRDFKGSFTSHQTVQP
                     NQAVAWNPSITGTKSEDTFLCTESGNVLVTQAKNWPMVSVSGFEFERPGILCL"
     gene            320..1108
                     /locus_tag="PKH07_19755"
     CDS             320..1108
                     /locus_tag="PKH07_19755"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNT37237.1"
                     /translation="MTIVALDSISLRRDQRTILDNVSWSIEKGQHWAVLGANGSGKTS
                     LLKVTIGDEWPTEGTVEVLGERFGACSLHDLRKKVGWVSSALEHKIHGWDTALDITLS
                     GFEASLGLYREFTDEELGLAMHSLSLVRAADYADAEYGKLSQGEQQRVLIARALVNRP
                     ALMILDEPCAGLDPVARAVFLGDLQKLAGRADSSAMVLVTHHIEEIDDWITHVLLLKA
                     GRAIASAPKKDILGSAELLSDVFGAKCTVTRADGRYFLHVQNTH"
BASE COUNT          263 a          288 c          331 g          254 t
ORIGIN      
        1 gaaaagggcg tggaggccta caaagccgtc ggttttcctg acgaatggaa actccaccat
       61 caggggggcg caacggggta tgagggacgt gatttcaaag ggagtttcac atcccatcag
      121 accgttcaac ccaatcaggc tgtcgcatgg aatccgagca tcacaggaac caagagtgaa
      181 gatacgttcc tctgcaccga gtcggggaat gttctggtca ctcaggcgaa gaactggccg
      241 atggtgtctg tttcaggctt cgaattcgag cgcccaggga ttctgtgcct ctgaaccaca
      301 ggaggttgac tacgctgaca tgacaatagt agcactggat tcgatctcac ttcgtcgcga
      361 tcaacggact attctggaca acgtctcatg gtctatcgag aagggacagc actgggctgt
      421 tcttggggca aatggttcgg gaaagacatc tcttctgaag gtgaccattg gcgatgaatg
      481 gccgacagag ggaaccgtcg aggttcttgg agagcgtttt ggagcgtgca gtcttcatga
      541 tcttcgcaag aaagtcggtt gggtgagttc ggctttggaa cacaagatcc acggctggga
      601 cacggccttg gacatcacgc tgtccggatt tgaagcctca ctggggctct atcgggagtt
      661 cacagatgaa gaacttggcc tggcaatgca ttcgctgtcg cttgttcgtg cggcagacta
      721 tgcggacgcc gagtacggaa aactctctca aggtgagcag caaagggttc tcatcgcccg
      781 agcgctggtc aaccgtcctg cgctgatgat tctggacgaa ccctgcgctg gactggatcc
      841 tgtcgcccgg gctgtattcc ttggagatct ccagaaactg gcgggacgag cggattcttc
      901 cgccatggtt cttgtcacgc atcatatcga ggaaatagac gactggatca ctcatgtcct
      961 gctattgaaa gcaggccgag ctattgcctc ggctccgaaa aaggacattc tgggatcggc
     1021 agaactccta agcgatgtct ttggagcgaa atgcaccgtc acccgcgctg acggcagata
     1081 ttttctccac gtccaaaata cccattgaca caccagcggg tgttctccca aggggg
//
LOCUS       DAOQUC010001542         1136 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_185708_length_1136_cov_2.167438, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001542 DAOQUC010000000
VERSION     DAOQUC010001542.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1136
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_185708_length_1136_cov_2.167438"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="PKH07_19760"
     CDS             complement(<1..222)
                     /locus_tag="PKH07_19760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37238.1"
                     /translation="MKSSVAVAMIVCGTILIAMPYIHNMIAMQQVAAAMVALNTTVNL
                     TADIPKHADEFCMFGGIVMIVVGAVAGLRN"
     gene            complement(435..>1136)
                     /locus_tag="PKH07_19765"
     CDS             complement(435..>1136)
                     /locus_tag="PKH07_19765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37239.1"
                     /translation="TGCFGQAFDAVQTPKGHSRKIGGRTLEYFDRSAGKISLERQQTV
                     TSGLEQFLASGAPELPKQESFRFGAEDGDITTVLRCSGEPLLSIDRKEQIIFIHGSLF
                     AGACFDPDRQPPALFGSADASCNEHDPWGPYSASHPQNGFTQKLFQNILDYAKVDYRI
                     PNPEPRTLAPYLGDHMETVSISANIVYNNMPETRSIVVRTPFPPKGYSSTKTKGRYET
                     TVTVPAFEYVALEGF"
BASE COUNT          246 a          305 c          305 g          280 t
ORIGIN      
        1 gttgcgcagg ccagcgaccg ctccgacgac tatcatgacg ataccaccga acatacagaa
       61 ctcatcggcg tgctttggga tgtcggccgt caagttcacg gttgtgttca gggcgaccat
      121 ggcagccgca acctgctgca tggcaatcat gttgtgaatg tacggcatcg cgatcagaat
      181 cgttccgcag acgatcatgg caactgcaac tgatgatttc atgagtctcc ttcctttcat
      241 ccaggaaaag tgcttgcgtt ttctcttcag aaactttggg ctgcacctca cgaattctga
      301 atcatatcgt atgcctctcc cgctcgttgt caagccttcg tgacattgtc tcgacctatg
      361 gcagggaata gggtcaattg acgagatgcg tcaattgagc agtggggctt tggaagatcg
      421 gcatctttcc tctgtcagaa cccttccagt gccacatatt cgaacgctgg cactgtaact
      481 gtcgtctcat atcgcccctt ggtcttggta ctgctgtagc cttttggggg gaacggagtt
      541 ctaacaacaa tagagcgtgt ttcgggcatg ttgttgtaga cgatgttcgc cgagatggaa
      601 acagtctcca tgtgatcacc cagataaggc gcgagggttc tcggctccgg atttggaatg
      661 cggtagtcca ccttggcata gtcgagaatg ttctgaaaga gtttctgggt aaagccgttc
      721 tgcggatgac tggcactgta cggtccccag gggtcgtgct cattgcagga cgcgtctgca
      781 gaaccgaaga gcgcaggcgg ctgtcggtcg gggtcgaaac aggcacccgc aaaaagcgaa
      841 ccatggatga agatgatctg ttcctttcgg tcaatgctca acagcggttc acccgaacat
      901 cgcaggacgg ttgtgatgtc cccgtcttct gctccgaatc ggaaagactc ctgctttgga
      961 agctccggtg cgcctgacgc gagaaactgt tcaagcccac ttgtcacggt ctgttggcgc
     1021 tcaagactga tcttccccgc cgaacgatca aagtattcga gcgttcgacc accgatcttt
     1081 cgactgtgtc ctttcggcgt ctgcaccgca tcaaacgcct gcccaaaaca accggt
//
LOCUS       DAOQUC010001543         1136 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_185808_length_1136_cov_1.203515, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001543 DAOQUC010000000
VERSION     DAOQUC010001543.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1136
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_185808_length_1136_cov_1.203515"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..533)
                     /locus_tag="PKH07_19770"
     CDS             complement(<1..533)
                     /locus_tag="PKH07_19770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37240.1"
                     /translation="MSDIKLACPDCKQHIAAPEEMAGQSVNCPTCNKILQIPERSTVI
                     PVSQNPMPLSDVAPSGSPQTAPTTDAGVGDTRKNQVATNLTEMAKTTIGAAQHLADQG
                     TSIAKNKWKDVGGTEGVKQKAKTYVEAVKHGFAPDEGAKGVGQVTSRVKNLWRSSKYG
                     KVTIIAASLILCWLLFGG"
     gene            complement(643..>1136)
                     /locus_tag="PKH07_19775"
     CDS             complement(643..>1136)
                     /locus_tag="PKH07_19775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37241.1"
                     /translation="AQTKEKRINEQYHHSLDFLMQSLTRSGKLDAALAVRDEMNQVAK
                     TLGMTNATVVSKIGGLQEVSKTVEAERRLVSLMKPYPSSYKDAPTDRMQVQYAIIELG
                     MQVGLQYDFNQSAKNTDNVCRQYVTPKIKSISFQAAMREILNPLGLTYDLRDGTTIVL
                     KKK"
BASE COUNT          223 a          291 c          289 g          333 t
ORIGIN      
        1 ccgccgaaaa gcaaccaaca gaggataaga gatgcagcga tgattgtgac cttgccgtat
       61 ttgctgctcc tccaaagatt cttgactctg ctcgtgactt gcccaacacc ctttgcgcct
      121 tcatctgggg caaacccatg cttcacagcc tccacatagg tcttggcctt ctgcttgacg
      181 ccttcagttc caccaacatc tttccatttg ttcttggcaa tactcgtacc ttggtctgcg
      241 agatgttggg ctgcgcctat ggtggtcttc gccatttctg tgaggtttgt tgccacttgg
      301 tttttccgcg tgtctccgac tccagcatca gtagtcggtg ctgtctgggg agaaccagag
      361 ggcgcaacat cagatagtgg catgggattc tgagatacag gaatgacagt cgatctctcc
      421 ggtatctgga gaatcttgtt gcatgtgggg cagttcactg actgtcccgc catttcttct
      481 ggagccgcaa tgtgttgctt gcagtctgga cacgctagct tgatgtcgct catctggtgt
      541 cttcctctct cttgcgaacg tcagcgacca ccgaacccgt gagccgaagg cgaacgaggg
      601 ttcggtgtgt cgccttgttc gcacattttg gcatcgagta actcacttct tcttgagcac
      661 aattgttgtg ccgtcgcgaa ggtcataggt caatcccagt ggattgagta tttctctcat
      721 cgccgcctga aaacttatgc ttttgatttt tggagtcaca tattgacggc aaacgttatc
      781 ggtgttcttg gccgattggt taaagtcata ttgcaggccg acctgcatcc cgagttcaat
      841 tatggcgtac tgaacttgca ttctgtccgt tggagcatct ttgtaggagg atggataggg
      901 cttcatcaga gagacaagtc ttcgctcggc ttcaactgtc ttggatactt cttgaagacc
      961 accgatcttg ctcacgaccg tcgcgttggt catgccaagc gttttggcaa cctgattcat
     1021 ctcatcccga acagcaagtg ctgcgtcaag ttttccactc cgcgtgaggg actgcatgag
     1081 gaaatcaagc gagtggtggt actgttcgtt gatgcgcttt tctttagtct gtgcca
//
LOCUS       DAOQUC010001544         1135 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_185898_length_1135_cov_2.799074, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001544 DAOQUC010000000
VERSION     DAOQUC010001544.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1135
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_185898_length_1135_cov_2.799074"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            94..>1135
                     /locus_tag="PKH07_19780"
     CDS             94..>1135
                     /locus_tag="PKH07_19780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37242.1"
                     /translation="MDDAFVESAAYILSKRSDSQLMAIDLRGFVGLQSKLAESIVAVE
                     RDSRSANWVIKNRELIESLPDSKLLYSLPIRVHRLLGEACVLEPDVATVRQGMGTFND
                     FRFLRLRHEVSVADIHRSGWEPLAKGGAFSFYYSDIHLLLNWKDDGAELSAVNIAHNG
                     QDAQVRQASEYWRRSGLTYSKRSQKGFSARALPAECIIAGKGPAILSQSDMSAAYLLG
                     WVNSRFIRWIIEVQANDHEYNTGIVKKVPWVQPACADHELTERTRDVVCRLQYARSMD
                     ETNSCFCPVFDAGSLRKSLEKWSSLNRDAQVALSNIMAEWDRFIDDLYGVDSQCLQID
                     DSDGDAPKEEEDE"
BASE COUNT          283 a          240 c          304 g          308 t
ORIGIN      
        1 cgttcattta tgacaatctc acggctacga cagtaccgtc ggaagattat tctgcctcaa
       61 atttctttgt tagccgatct cgggtcctcc gtgatggacg acgcctttgt ggaaagtgct
      121 gcgtacatcc tgtcgaaacg atctgatagc cagttaatgg ctattgattt aagaggattc
      181 gtggggcttc aatctaagtt ggcggaaagt attgttgctg ttgaaagaga cagtcgttcc
      241 gcaaactggg tgattaagaa tcgagaattg atagagagtc ttccagacag taaactgctc
      301 tattctcttc ctattcgtgt acacaggctg ctgggcgaag cctgtgttct tgagccagat
      361 gtagcgacgg taaggcaagg gatggggaca ttcaatgact ttcgttttct gaggcttcgc
      421 cacgaagttt cggttgctga catacatcgg agtggttggg aacccttggc aaagggtggc
      481 gctttttctt tttactactc cgacatccat cttcttttaa actggaaaga cgatggtgcg
      541 gaactgtccg ctgtgaacat cgcccataac ggtcaagatg cccaggttcg tcaagcttcg
      601 gagtactggc gaaggtccgg attgacatat tcgaaaagga gccagaaggg cttcagtgca
      661 agggcattgc cagcagaatg cattatcgcc ggcaaaggac cagctattct ctcgcaatct
      721 gatatgtcgg ctgcatattt gcttggttgg gtcaactctc gcttcatccg ttggattatt
      781 gaggtacaag cgaatgacca tgaatataac acaggcattg tgaaaaaagt accatgggtt
      841 caaccggcgt gcgctgacca cgagttgaca gagcgaacaa gagatgttgt gtgcagactc
      901 caatatgcaa ggtctatgga cgaaaccaat tcatgttttt gtccagtttt tgacgctggt
      961 tcccttcgga agtctctcga aaaatggagc tccctcaacc gcgatgccca agtggctttg
     1021 tctaatatca tggcggagtg ggatagattc attgatgatc tgtatggtgt agattcccag
     1081 tgcttgcaga ttgatgattc agatggggat gcacccaagg aggaggaaga cgaag
//
LOCUS       DAOQUC010001545         1134 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_186125_length_1134_cov_3.801668, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001545 DAOQUC010000000
VERSION     DAOQUC010001545.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1134
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_186125_length_1134_cov_3.801668"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..658
                     /locus_tag="PKH07_19785"
     CDS             <1..658
                     /locus_tag="PKH07_19785"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNT37243.1"
                     /translation="WVLDLCEAMDRDVPGLRWICNSRVDAVDEEILAAMKKAGCLLIS
                     YGLESGSQSILDSCRKGISLDDSRRAIHLTRRAGILAFGYFILGLPEETWETVRQTID
                     FALDVEPDYALFHIATPFPGTRLFDEAVEKGLLLNQDWDLCDEETAGILRTVALSAED
                     LAKARRIAMREFYLRPKVIARELTRTASVGSIVSRTEAAARMLLNWAGKKHPCKGLTR
                     "
     gene            655..>1134
                     /locus_tag="PKH07_19790"
     CDS             655..>1134
                     /locus_tag="PKH07_19790"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HNT37244.1"
                     /translation="MTEKDKSVVIKAKLQDIKDLPSLPLAADSITRAVLDDKSTAQTI
                     ATAISKDPSFASRILRVVNSAYYGFYRQITSIKEAVALLGTNEIRMMAMAITVYDLFK
                     AKEATRFNRKNLWMHGVAVAYMADYLQRTLRKGLDEAYVAGLLHDIGKIVLDQFFPEE
                     "
BASE COUNT          269 a          284 c          323 g          258 t
ORIGIN      
        1 gtgggtattg gatctctgcg aggccatgga tcgtgatgtt cccggccttc gctggatctg
       61 taacagccgc gtggacgctg tggacgagga gatcctggcc gcaatgaaga aggcaggctg
      121 cctgctgatc tcctacggtc tggaatctgg aagccagagc atcctcgaca gttgtcgcaa
      181 ggggatttca ctcgatgatt ccaggcgagc catccacttg acccgacgag caggaatttt
      241 ggctttcggc tacttcatcc tggggcttcc ggaagagact tgggagacgg ttcgtcaaac
      301 gatagacttt gcgctggatg ttgagcccga ctacgcgctc ttccatatcg ccactccttt
      361 cccgggcact cggctgtttg atgaggcggt ggagaagggg ttgttactca atcaggattg
      421 ggacttgtgc gatgaggaga cggcaggtat actcagaacg gtcgcattat cggcggagga
      481 tctcgccaag gcgagaagaa tcgcaatgag agaattctat ctacgtccca aagtcatagc
      541 aagagaactc acgcgaactg cttcggtggg cagcatcgtt tccagaactg aggcagcggc
      601 gagaatgttg ctgaattggg caggcaagaa acatccatgt aaggggttga ctcgatgacc
      661 gaaaaagaca agtcagtagt aatcaaagcg aaacttcagg acatcaagga tctgccatcc
      721 ttgccgttgg cggcggacag catcacccgt gctgtcctgg atgacaaatc caccgctcag
      781 accatcgcaa cggcgatctc aaaggatccg tcctttgcct ctcggattct tcgcgtggtg
      841 aattcagcct actatggttt ctacagacag atcacctcga tcaaggaagc ggtggctctt
      901 ctgggaacaa acgagatcag gatgatggcc atggccatta ccgtctacga tcttttcaag
      961 gccaaagaag ccactcgatt caaccgaaag aatctctgga tgcatggtgt tgccgttgcc
     1021 tatatggcgg actaccttca gcggacactc cgaaaaggcc ttgatgaagc ttacgtggcc
     1081 ggcttgttgc acgacatcgg caagattgtt ctggatcagt tcttccctga ggag
//
LOCUS       DAOQUC010001546         1132 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_186730_length_1132_cov_2.897864, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001546 DAOQUC010000000
VERSION     DAOQUC010001546.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1132
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_186730_length_1132_cov_2.897864"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..710
                     /gene="pdxA"
                     /locus_tag="PKH07_19795"
     CDS             <1..710
                     /gene="pdxA"
                     /locus_tag="PKH07_19795"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:TIGR00557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HNT37245.1"
                     /translation="SIKVAVDMAMHGLVQGIVTGPICKQSLQLAGCPHAGHTELLAEL
                     GKVKRTVMMLANAKLRVVLVTTHIPLKDVAKEITCDHVLETIEITTQTFQRFGWGTPR
                     LGVLGLNPHSGEQGTIGTEEDVEIIPAVTEARRRGCVCLGPIPADTAFHRALNGEFDV
                     VVAMYHDQGIGPLKTLSFSDVINVTLGLPFIRVSPGHGTAFDISGTGTANPASMKLAL
                     ETAVDLCARMPTESSDK"
     gene            901..>1132
                     /locus_tag="PKH07_19800"
     CDS             901..>1132
                     /locus_tag="PKH07_19800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37246.1"
                     /translation="MKRYAFLTGLIASVVACCSLAGESFGQAEGVSEPLAGMCSLSGI
                     VRDASDTAIADAKLSLFIFEAPEHEWKAQTPAD"
BASE COUNT          274 a          289 c          319 g          250 t
ORIGIN      
        1 agtccatcaa agtggctgtg gatatggcca tgcacggtct tgtgcaaggg attgtgacgg
       61 ggccaatctg caagcagtca ctccaactgg caggatgccc ccacgcgggt cacacggaac
      121 tgctggcgga actgggcaag gtgaagagaa cagtgatgat gttggccaat gccaaactga
      181 gagttgtgtt ggtcacgacg catattcccc tcaaagatgt cgccaaggag atcacctgcg
      241 accacgttct ggagacaatc gagatcacga cacaaacgtt tcagcggttt ggatggggaa
      301 cgccccgact gggggtactt ggcctgaatc cacactccgg agagcaggga accattggta
      361 cagaggaaga tgtcgagatc atcccagcgg tgaccgaggc ccgtcgaagg ggatgcgtgt
      421 gtcttggtcc cattcctgcc gacaccgctt tccatcgtgc cttgaatggg gaattcgatg
      481 tggttgtagc catgtaccac gaccagggaa taggcccgct caagacgctt tcgttttcgg
      541 atgtcatcaa cgtcacgctc ggactgcctt ttatccgagt ttctcccgga catggcaccg
      601 cgttcgacat ctccggtacg ggaaccgcaa atccggcaag catgaaactg gctttggaaa
      661 cggcggtgga tctgtgcgcg aggatgccaa cggaatcctc agacaaatag agccaaccgg
      721 tcgcagcatc ccagaaacaa acgactcatc accagagatg gcgagacata gtatcgaaga
      781 tgatctattt gcagatctct ttgaatactg acagaataat ctgagtagta agactacaga
      841 gccgtgtgta tcgaaatgct ttccgtcatt ggaaccgttc gtagaaagag gtactcaccc
      901 atgaaacgct atgccttcct cactgggctg atcgcatcag ttgttgcctg ttgttctctc
      961 gccggggagt cttttggtca agcagagggg gtctctgagc ctttggcggg catgtgttcc
     1021 ctgtcaggga tcgttcgaga cgcttcggac actgcaatag cagatgcaaa actgtctctc
     1081 ttcatctttg aagcgcctga gcacgaatgg aaggcacaaa cacctgcgga tg
//
LOCUS       DAOQUC010001547         1131 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_187054_length_1131_cov_2.380112, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001547 DAOQUC010000000
VERSION     DAOQUC010001547.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1131
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_187054_length_1131_cov_2.380112"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1131)
                     /locus_tag="PKH07_19805"
     CDS             complement(<1..>1131)
                     /locus_tag="PKH07_19805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043163 - cell envelope organization
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HNT37247.1"
                     /translation="WNGTPLKQKVAGADLLPLFCERAAQKQHRVFLLGGTEGVPERAA
                     NNLCSQYPGLRVVGTYSPPLQFEQDPIQNEKAISLVRKASPEILFVALGTPRQEKWLY
                     RNLEQLRVPVCIGVGAGIDFAAGEQKRAPLWIQRHGLEWLYRFIQEPRKIWRRVLISI
                     PLFIAMFIDLRTYSTQKRSIGMIVTSVKLVFVAALILLSYCFSVWIRHSNAPPPFLPP
                     WTGFYVPYQNLMPIVILSYLFACLHFGFFRSKSGEGPLEILKSSFMASSLGTVAVLMS
                     SFLFKEIYIHEITGFSRISIGLTWFISGILFFAWRITVWLIAKALARNNLLMERILIV
                     GTAPESARFIRGLCHDRFCRVKAIGIVVEKEQEAEYYEGLPIL"
BASE COUNT          325 a          293 c          269 g          244 t
ORIGIN      
        1 agaatcggca gaccttcgta gtattctgct tcctgttcct tctcgacaac aattccaatc
       61 gccttcacac gacagaatcg gtcatgacac agtccacgaa tgaatcgagc agactccggt
      121 gctgttccta cgataaggat ccgctccatc aaaagattat ttctggcaag agccttggcg
      181 atgagccaca ctgtgatgcg ccaagcaaag aagagaatgc cactgataaa ccaagtcagc
      241 ccaatactaa tgcgtgaaaa gccagtgatc tcatggatat agatttcctt gaatagaaaa
      301 ctgctcatga gaaccgccac cgtgcccagg gaacttgcca taaaactgct cttcagaatc
      361 tccaacggtc cttccccact cttgctccga aagaatccaa agtgaaggca ggcaaagaga
      421 tacgacagga tcactatagg catcaggttt tggtagggaa cgtaaaaccc agtccaaggg
      481 ggcaagaaag gaggaggggc gttgctatgt cgaatccaca ctgaaaagca atacgacaga
      541 agaatcaagg ccgcgacaaa caccaacttc acggaggtga caatcatccc gattgagcgc
      601 ttctgcgttg agtaggttct gagatcgata aacatcgcaa taaagagagg gatcgagatc
      661 agtacgcgac gccagatttt gcgaggttcc tggataaacc ggtagagcca ttccaagcca
      721 tgacgctgga tccacagagg tgcccgcttc tgctctccag cggcaaagtc aatcccagca
      781 cccactccta tacaaactgg aactctcaac tgctcaagat tcctgtagag ccatttctcc
      841 tgtcgcggtg tgccgagagc aacgaaaagg atttcaggac ttgctttgcg aacgagagag
      901 atggccttct cattctgaat gggatcctgc tcaaactgta gtggaggact ataggttcca
      961 acgactctca aaccagggta ttgagagcag agattgttgg ctgcacgttc tggcacgcct
     1021 tccgtaccac ccagaaggaa tactctatgt tgcttttgtg cagcccgttc acagaaaaga
     1081 ggaagcagat ccgcaccagc cactttctgc ttcaggggag ttccattcca t
//
LOCUS       DAOQUC010001548         1130 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_187336_length_1130_cov_2.266047, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001548 DAOQUC010000000
VERSION     DAOQUC010001548.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1130
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_187336_length_1130_cov_2.266047"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..862
                     /locus_tag="PKH07_19810"
     CDS             <1..862
                     /locus_tag="PKH07_19810"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF006054.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HNT37248.1"
                     /translation="TVRRAAQCLLERGFPAPRVGVIAGSGISDAITELLVDPRRADAA
                     EIPGVPSVQVLGQSNHWTAGFVQEIPILVFGGRKHLYEQVPIHEVIFPILLLAELGAD
                     GVILTNAAGAVNPNFHVGQMMLIRDHIDSSFGQFADELWQVLQGFEPMGSSGCPYDVE
                     WLSAAAAEARSQHVDCAEGIYCFNRGPFYETPAEVIACRHMGADAVGMSTVPEAIAAH
                     ALGLRVLAVSLLTNMATGMTSQAHSHDEITRQAAKAHRDIAQLLDSLLKHRLTGAAID
                     TADGFQTHNT"
BASE COUNT          260 a          280 c          323 g          267 t
ORIGIN      
        1 gactgtccga cgggcagcac agtgtcttct tgaaagagga tttcctgctc cgcgagtggg
       61 agtgattgcc ggttcaggga ttagcgacgc gatcacggaa cttctcgtcg acccacggag
      121 ggctgatgcc gcagagattc caggggttcc gtcagtccag gttctcggac agtctaacca
      181 ctggacggct ggcttcgtgc aggagattcc gattctggtt tttgggggca ggaaacatct
      241 ctacgaacag gtgcccatcc acgaggtgat ctttcccatt cttttgcttg cggagttggg
      301 tgcggatggg gtcattctga ccaacgcagc aggagcagtg aatccgaatt ttcatgtcgg
      361 tcagatgatg ttgatcagag accacattga ctcatcgttt gggcaatttg cggatgaact
      421 ctggcaggtg cttcaaggtt tcgaaccgat gggctcgtct ggttgtccct atgacgtcga
      481 gtggttgtca gcagcggcgg cagaggctcg ttctcagcac gttgactgcg ccgaagggat
      541 ttactgcttc aaccgagggc ctttctatga gaccccggct gaggtcatcg cctgtcgcca
      601 catgggtgcg gatgccgtgg gcatgtccac cgttcctgaa gcgatagcag cgcatgcgct
      661 gggcttgaga gttcttgcgg tatcccttct gacaaatatg gctaccggca tgacctctca
      721 agcacacagc cacgatgaaa tcacccgaca agcagcgaaa gcgcacagag atattgccca
      781 acttctggac agcctgctga agcacagact gaccggagct gcgattgaca cagcagatgg
      841 atttcaaacc cacaacactt gagcgaactc attgacggat gatttgggat caactacatt
      901 gatcttacgg tacgtacctg ttgtgaaaca atcctcttgg tgcactcttt cgtcattggg
      961 gagttcgcca aggctagaga cagagcactt cacgcaacag agcgattgtc ggggtactca
     1021 tatgtgaaga tagcaataat ctttgcccat gttctgaagc agttaccata cggagacaga
     1081 ggaggaggca gagatgccga tgcattcgat aaacttcgat tacgggaata
//
LOCUS       DAOQUC010001549         1128 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_187821_length_1128_cov_2.758621, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001549 DAOQUC010000000
VERSION     DAOQUC010001549.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1128
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_187821_length_1128_cov_2.758621"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="PKH07_19815"
     CDS             complement(<1..312)
                     /locus_tag="PKH07_19815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37249.1"
                     /translation="MRETNASMKIGLARVDVTPPLTIPYLSFHPRQTPFKGVRDRLHA
                     RALVAESDSGRIALVSVDALGFSRSILGPGRDFIEEVRQEVQRRTGIPAGNVLLASTH
                     AH"
     gene            complement(793..>1128)
                     /locus_tag="PKH07_19820"
     CDS             complement(793..>1128)
                     /locus_tag="PKH07_19820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37250.1"
                     /translation="RGVVFLCPDTEIHQDPLCVYRPEKSKRPVIRNGWFKGDDGREQP
                     WSIGLEVALPPSSLDIDLGICPEQIFDFVLGFVTLDPLADDRVGLDEEDLTLWFKQNE
                     DWPEKRKCD"
BASE COUNT          318 a          298 c          281 g          231 t
ORIGIN      
        1 atgtgcgtgc gtcgaggcca agagcacatt cccagcggga atgcccgtcc gacgctgaac
       61 ttcctggcga acttcttcta tgaaatcgcg acccggaccg agaatgctac ggctgaaccc
      121 aagagcgtcc acagaaacaa gggcgattcg tccactgtca gattccgcca ccaacgctcg
      181 ggcatgaagt ctatcgcgaa cgcctttgaa tggagtctgt ctggggtgaa aactaagata
      241 aggaatcgtc agtgggggag tgacatccac ccgagcaaga ccgatcttca tgcttgcatt
      301 tgtctctctc atcaaaactc ctgggaaacc agactctgaa gaagtccgtt tcttccgatg
      361 agcaagggtc tgtaagagtg ggcacagcgc acacgcctgc aggcgctagc cagctccgta
      421 gtaaccaagg tagcaatggt aacctgagcg ttcatagaag cacttgcatc ggaacgccag
      481 atatgacagg cgtgcacctg caaagcagca ctccctgttc agattcactt tgttaagaga
      541 acataagccg gccaaatcgt caagtcaatt gcgtcgcaga acctatagag gagaacaagc
      601 aaatccccag ctcatgttga agggagtgag gtcttcaatc caagccgaaa tcggcttgga
      661 ttgaagacct cggttcaaag aaagacgtcc ctcttttatc tatctgacac cgatcaggac
      721 agaaatcctc acgggcgact gaggaaaagg ttgtgccaaa gtcctcaatg aagaagacgg
      781 caaccgagat gatcagtcac actttctttt ctcaggccaa tcctcgttct gcttgaacca
      841 aagagtgagg tcttcctcat cgagaccgac tcgatcatct gccagaggat ccaaggtcac
      901 aaagccaaga acgaaatcga aaatctgctc ggggcaaatg ccgagatcta tgtccaaaga
      961 cgaaggggga agagcgacct caagaccaat gctccaaggc tgctcccgac catcgtcgcc
     1021 tttgaaccaa ccatttcgga tgaccggtct tttggacttc tcgggacgat aaacacaaag
     1081 gggatcctga tggatttcag tatctggaca aaggaaaact actcctcg
//
LOCUS       DAOQUC010001550         1127 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_188060_length_1127_cov_3.447761, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001550 DAOQUC010000000
VERSION     DAOQUC010001550.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1127
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_188060_length_1127_cov_3.447761"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..609)
                     /locus_tag="PKH07_19825"
     CDS             complement(<1..609)
                     /locus_tag="PKH07_19825"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNT37251.1"
                     /translation="MQEILNVLITDDEPGMRLAVSRVLRDYRFTLAEVGSEIAFAVSE
                     AETGEQALDLLRQTPVDILLLDCRLPGRSGLDVLTELSEHPRDLITVMITAYASIEAA
                     VTATKKGAFDFLAKPFTPDELKNVIQKAARQLILQRQTRRLLEEKNQVRFQFISVLAH
                     ELKAPLAAVEGFLKIIRDRTAGNDEQIYRQMIDRSLIRLDGMR"
     gene            complement(615..1022)
                     /locus_tag="PKH07_19830"
     CDS             complement(615..1022)
                     /locus_tag="PKH07_19830"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNT37252.1"
                     /translation="MSSQERMSGTILIVDDDPDFRLQERIQLEAVGFTVLEAETESEA
                     RRIFQERTPDLAIVDLMLENMDDGFMLCHFIKRKFPSVPVIMVSAVTSETGIGFDAST
                     DEERSWIKADAFLSKPLRFEQMFREIKRLLADE"
BASE COUNT          283 a          327 c          252 g          265 t
ORIGIN      
        1 tcgcattccg tccagacgaa tcagactccg atcaatcatc tgccggtaga tctgctcatc
       61 gttccctgcc gttcgatcac ggatgatctt taggaatcct tcaaccgccg caagtggcgc
      121 tttcagttca tgagcaagaa cagagatgaa ctggaagcga acctgattct tctcttccaa
      181 aagccttctc gtctgccttt gcaggatcag ctgacgtgcc gccttttgga tcacattctt
      241 gagttcatcg ggtgtaaatg gctttgccag aaagtcaaag gcgcccttct tggtagcagt
      301 cacagccgct tcaatcgagg cataggcagt gatcatcacg gtgatcaagt ctcttggatg
      361 ctcgctcaac tcagtcaata catccagacc gcttcttccg ggaagtcggc agtccagaag
      421 aagaatgtca acaggtgtct gacgcaacaa atccagcgcc tgctcccctg tctccgcttc
      481 ggagaccgca aacgcaattt cggatcccac ctcagcaagg gtgaagcgat agtcccgaag
      541 aacgcgactg accgccaggc gcatgccggg ctcgtcatcg gtgatcaaga cattcagtat
      601 ctcttgcatg gcactcactc atccgccaag agacgtttga tctcacgaaa catctgctcg
      661 aaacgcagag gcttgctcag aaaggcatcg gccttgatcc acgatcgttc ttcatcggtt
      721 gaggcatcaa aaccgatgcc cgtctcactc gtcacagcac tgaccatgat gacaggaact
      781 gaggggaact tcctcttgat gaaatgacag agcatgaaac cgtcatccat gttttccagc
      841 atcaaatcta cgatcgccag atctggtgtg cgttcctgaa agatccgacg cgcttcagac
      901 tcagtttccg cctcgagcac agtaaaaccc accgcttcca actggatgcg ctcctgcaac
      961 ctgaaatcag gatcatcatc cacaatcaga attgtgccac tcatccgttc ttgtgatgac
     1021 atcgtccttg tcaaaccttt ctgaactcaa cgaccatggg cgtgcaaaag tctaaaggat
     1081 tctctcatga ccatccctat caaagcgcat cagaacgctt gtgatag
//
LOCUS       DAOQUC010001551         1127 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_188066_length_1127_cov_3.190299, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001551 DAOQUC010000000
VERSION     DAOQUC010001551.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1127
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_188066_length_1127_cov_3.190299"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1127)
                     /locus_tag="PKH07_19835"
     CDS             complement(<1..>1127)
                     /locus_tag="PKH07_19835"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNT37253.1"
                     /translation="EIAWKQVQSDSKVGTLTLENARKGIQSLTYLESAARILSARGHQ
                     RESIDLCLSILQKTEWLLSESEKEDSVWASVQSDTLEKETAVRSLVAPLLQSVGRESE
                     AIEIDLEAARRFFEKGQLDSADSVLERVLTSAPDQIQARLLMIEVLTQQYLNEQTQTQ
                     DQSMYTGSVLMLRTPSENEIEVQHRIAEHRLALARVYLDKGDRTQAIQQYEILLQIAP
                     SDGDAREELISLYLERGEMEKGVAQLRSLANTYREKGLLGNVLEIYHRALDIDPNNHD
                     LAIELAKVYGEQGLTDKAIRLLRRVSDTEWAKGELGQATQSLKQAVEIEPTNVTLLGK
                     LSKGLLMLGDLDGAREAYTSLMEVYCERGLFARAAQMIDKD"
BASE COUNT          259 a          310 c          275 g          283 t
ORIGIN      
        1 gatccttgtc aatcatctgt gcggctcggg caaacaaacc tcgctcgcaa tagacttcca
       61 tgagggaagt gtaggcttca cgcgctccat ccaagtctcc gagcatgaga agtcccttgg
      121 acagtttgcc aagcagtgtc acgttggtcg gttcgatttc gaccgcctgc ttcagacttt
      181 gtgtggcctg gccaagctcc cctttcgccc attccgtatc cgaaacacgt cgaagaagtc
      241 ggatggcttt gtctgtaagc ccctgctctc cgtagacctt ggccagttcg atggcaagat
      301 catgattgtt gggatcaatg tctagagcac ggtgatagat ctccagcaca ttgcccagca
      361 atcctttctc gcggtaagtg tttgcaagac tcctgagttg ggcaacaccc ttctccattt
      421 ctccacgttc caggtagaga gaaatcagct cttcccgagc atcaccatca gacggagcga
      481 tctgaagaag tatttcatac tgctgaatcg cctgcgttcg gtcgccttta tccagataga
      541 ctctggccag agccaaacga tgttctgcga tccgatgctg gacttcaatc tcattctccg
      601 agggggtgcg aagcatcaac acagaacccg tgtacataga ctgatcctga gtttgggtct
      661 gctcattcag gtactgctgg gtcagaacct ctatcatgag aaggcgtgcc tgaatctggt
      721 caggcgcact ggtgagaact cgctccaaga cggagtccgc actgtcgagt tgtccctttt
      781 caaagaaacg tctggccgcc tccagatcaa tctcaatggc ttcagattca cgaccgacag
      841 actgaagcaa gggtgcaaca agtgaccgaa cagcagtttc cttctcaaga gtgtcactct
      901 ggacggatgc ccagacagaa tcctccttct cggattctga aagcagccat tccgtcttct
      961 gcaaaatgct aaggcagagg tctatggatt cgcgctgatg tcctcttgca gaaaggattc
     1021 tcgcagcaga ttcaaggtat gtcagtgact gaatcccttt gcgcgcattt tccagagtga
     1081 gtgtaccgac tttcgaatcc gactgcactt gcttccaggc aatctca
//
LOCUS       DAOQUC010001552         1125 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_188576_length_1125_cov_4.035514, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001552 DAOQUC010000000
VERSION     DAOQUC010001552.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1125
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_188576_length_1125_cov_4.035514"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..389)
                     /locus_tag="PKH07_19840"
     CDS             complement(<1..389)
                     /locus_tag="PKH07_19840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37254.1"
                     /translation="MNMRRFFILGCLMIVALPLVQAEEGTGVNIGTGTILLLRKVERY
                     GAIVPREIDMSTGRFAFDWYLLPGDEKNFGSQDVQSGYNIVYLEDGKAAVSFATFALR
                     LVQGPGGQLLLRSATKPGLHTNNIYFS"
     gene            complement(389..>1125)
                     /locus_tag="PKH07_19845"
     CDS             complement(389..>1125)
                     /locus_tag="PKH07_19845"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="rRNA adenine dimethyltransferase family protein"
                     /protein_id="HNT37255.1"
                     /translation="ARQVFAVELDDGYCDFMVERFGAPCVAVQDLRDAAKPIQGHIVV
                     IRGSAMSFDPGWVAEERRGIVVYGNLPYCETTPILVHLLEHRRHIDRMLLLVQKEVGE
                     RIVAHPGAKDKAYGKLSVMVQTYFEPALLMDISPTVFLPRPEVRSAFLSLVTRENPFG
                     HLGSPLPDDFPEFLHRVVFQAFAKRRKMLRTLMPEGWPSVSAEDWNRVFADMGIDSSR
                     RGESLSPQMFHRMALTLYGIVYGSER"
BASE COUNT          289 a          328 c          273 g          235 t
ORIGIN      
        1 aaagaaaaat agatgttgtt ggtgtgtagc ccaggcttgg tggcggagcg cagcaagagt
       61 tgaccaccag gcccttgcac gagtcgcaac gcaaacgtgg cgaaactgac cgcagccttg
      121 ccgtcctcaa gatagacaat gttgtaaccc gactgaacgt cctgcgagcc gaagttcttc
      181 tcgtcaccag gcagcagata ccaatcaaag gcgaaccggc cggttgacat atctatctcc
      241 cgaggcacaa tcgcgccata gcgctccact ttgcgcagca gcagtatcgt tcctgtaccg
      301 atattgacac cggtaccctc ctctgcctgg acaagaggca aagccacgat catcaaacaa
      361 cccaggatga agaatcttct catgttcatt accgttcact cccatagaca attccgtata
      421 aggtcagcgc catccgatga aacatctgag ggctgagcga ttcgcctcga cggctgctgt
      481 caatgcccat atcggcaaaa acccgattcc agtcctctgc cgacacagac ggccaacctt
      541 cgggcatcag tgttcttagc attttccgac gttttgcgaa agcctgaaag acaactcgat
      601 gcagaaattc gggaaaatcg tctggcaagg ggctccccag atggccaaag ggattctctc
      661 gggtcaccag acttaagaac gccgatcgca cttcgggtct gggcaggaag acagttggac
      721 tgatatccat caacagtgct ggttcgaaat acgtctgaac cattacgcta agtttgccat
      781 aggctttgtc ttttgcccct gggtgagcaa caattcgttc tccgacttcc ttctgcacca
      841 aaagcagcat tcggtcaata tgacggcgat gctccagaag atgcacgaga attggtgttg
      901 tctcgcaata gggcaggttg ccatagacca caatgccacg acgctcttcg gcaacccatc
      961 ccggatcgaa actcatggcg ctgccacgga taacgacaat gtgaccctgt atcggcttgg
     1021 cagcgtcccg caaatcctga accgccacac acggcgctcc aaatcgctcg accatgaagt
     1081 cacagtagcc atcgtcgagt tccacggcga aaacctgtcg agcac
//
LOCUS       DAOQUC010001553         1124 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_188914_length_1124_cov_2.490178, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001553 DAOQUC010000000
VERSION     DAOQUC010001553.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1124
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_188914_length_1124_cov_2.490178"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..638)
                     /locus_tag="PKH07_19850"
     CDS             complement(<1..638)
                     /locus_tag="PKH07_19850"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HNT37256.1"
                     /translation="MFQKKMVFAFVSAVFAFASFTLRKVLSEHRKRRCLFAIGALFAF
                     LCLLSTSEADRSKIVVGTGDAFRPMVFLDENGLPAGRDVALWQLWSRKTGIEVEFRLT
                     EWSEVIPALLREEVDVVSGASYSPERGQLLAFTDPYQVLSAYAFFDQNTRPVSRLLDM
                     QGMTVGVLGGSTIEESLRQQVPEAILAPFPDYETMVSSIRGGRVNVLLGEEP"
BASE COUNT          286 a          298 c          287 g          253 t
ORIGIN      
        1 atgggctcct cacccagcag aacgttaaca cgtccacccc gtattgacga caccatggtt
       61 tcatagtcgg gaaagggtgc gagtattgcc tctggtactt gttggcgaag cgattcctcg
      121 atcgtgcttc cccccaacac accaacggtc atcccttgca tgtccagcaa tcggctcacg
      181 ggccgggtgt tctggtcgaa gaaagcgtaa gcggaaagta cctgataggg atcggtgaag
      241 gcgagcaact gcccgcgctc cggactgtat gaggcaccac tcacgacgtc cacttcttcc
      301 ctgagcaacg ccggaatgac ttccgaccac tctgtcaggc gaaactcaac ctcgatgccc
      361 gtctttcgag accaaagctg ccaaagggct acgtctcgac ctgcaggcag gccattctcg
      421 tccaggaaaa ccataggccg aaaagcatca cccgtaccca caactatctt gcttcggtct
      481 gcctcggatg tggacagcag acacaagaaa gcgaaaagcg ctccaatggc aaagagacat
      541 cttctctttc tgtgttcact caatactttc ctgagggtga aggatgcgaa ggcaaatacc
      601 gcagagacga aagcaaacac cattttcttc tgaaacatcc tgagactcct tccaagatat
      661 cttggcgagt tttgacatgc tttttactat agcgtctcaa gagttcctag gcaaaacaac
      721 tgggagaggc agctcagtaa cggactaagt tggtgcacaa gtactgagag agagaccttg
      781 ggctgcagaa aagtagatgt cagaagatcc gaggtgctct atggaaaaca gacagtcgaa
      841 tgagaggtgt catgcgacct gatccgcttg ctgtcgaagt agacccatgt gcaaggcatg
      901 ctcgaactgg acaggactgc tatagtccaa actggcgtgc attcggatag gattatggaa
      961 catttcgata tatctgaata tctctttcct ggttctggat caaggcgcct ggttcgtggt
     1021 ggttcttgga gcctcgtacc gagggactgc tgttccgcga acggggacga cgtcgccccg
     1081 gacaaccggt tattcctccc gaaaattcga atatcttgta tttc
//
LOCUS       DAOQUC010001554         1124 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_188929_length_1124_cov_2.414406, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001554 DAOQUC010000000
VERSION     DAOQUC010001554.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1124
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_188929_length_1124_cov_2.414406"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(109..543)
                     /gene="gcvH"
                     /locus_tag="PKH07_19855"
     CDS             complement(109..543)
                     /gene="gcvH"
                     /locus_tag="PKH07_19855"
                     /inference="COORDINATES: protein motif:HMM:NF002270.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HNT37257.1"
                     /translation="MKVREYEMPDDLYYHSEHTWLRVEDGKVRVGLNDWAQAAAGDIT
                     YVDLPFEGDSVEAGETCGKVQSSKWVGKLVAPVSGEIAEVNSELENDATLINQSCYGD
                     GWIMVIEPSSLEEDLGNLMQGSAAAEWLESEIQKVEKEKQGE"
     gene            732..>1124
                     /locus_tag="PKH07_19860"
     CDS             732..>1124
                     /locus_tag="PKH07_19860"
                     /inference="COORDINATES: protein motif:HMM:NF018613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37258.1"
                     /translation="MKVIIFYSKQHSGHHKAAEALAEGFRRWIPESTVVVQNSAEFFD
                     VRIGDLIEKAYMFTIRQMPWLWSWMYDNDRLKSRVKHVKRLNLFMNKRRLLRFIRDTE
                     PDLILCTEAVPCEMLCILKARGALSVPVI"
BASE COUNT          247 a          315 c          271 g          291 t
ORIGIN      
        1 ggagagccgc atcgtaaccg tagcagataa gatcctccag gtcggtataa acgcagcccg
       61 gactgagact gttccgaatc tgttcgatca gggactgctg catcatgatc attctccctg
      121 cttctctttc tccacttttt gaatctccga ctcaagccat tcggcggcag cgctgccctg
      181 catcagattc ccgagatcct cttccagact gcttggctcg atcaccataa tccagccatc
      241 gccgtaacag gactggttga tgagcgtcgc atcgttttcc agttcggaat tgacctcggc
      301 gatctctccc gacacgggcg caacgagttt tcccacccac ttgctggact gcactttgcc
      361 gcaggtctca cccgcttcaa cgctatcgcc ttcaaagggg agatcgacgt aggtgatatc
      421 tcccgcagcg gcttgtgccc agtcgttaag acctacgcga accttcccat cctccacgcg
      481 aagccacgtg tgttcactgt gatagtacaa atcatccggc atctcgtatt cacgaacttt
      541 catagccatg ctccttccgg cacaatctct tctgcacgca cggagcgtgc cccagcagtg
      601 tagttttcct gtattgtttg agaatcgtca agagaatttc gctgacactc gttttcgact
      661 gttgatcttc ccgctgaaca tgcagatatg ttatgttggt aggcacttag cgacggagag
      721 cagccaaaga gatgaaggtc atcattttct attccaagca gcattcaggc catcacaagg
      781 cggcagaggc gcttgcagaa gggtttcgac gatggattcc agagtcaacc gttgtcgtac
      841 agaattcggc tgaattcttc gatgtgcgaa ttggcgatct tatcgaaaag gcgtacatgt
      901 ttaccattcg acagatgcct tggctttggt cgtggatgta tgacaacgat cggttgaaat
      961 ctcgcgtcaa gcatgtcaag agactcaatc tgttcatgaa caagcggcgg cttcttcgat
     1021 tcatccgcga cacagaaccc gatctcattc tctgcactga ggcggtgcca tgtgagatgc
     1081 tttgcattct caaggcgcga ggcgcactgt ccgttcctgt gatc
//
LOCUS       DAOQUC010001555         1124 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_188944_length_1124_cov_2.308700, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001555 DAOQUC010000000
VERSION     DAOQUC010001555.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1124
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_188944_length_1124_cov_2.308700"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /locus_tag="PKH07_19865"
     CDS             complement(<1..278)
                     /locus_tag="PKH07_19865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HNT37259.1"
                     /translation="MVITDSLAKFPANPVETRWFTYENVTGEKGSAGKANYGRKGAPS
                     VALPSGSSLVLTDVKGSGTIRRIWMTLWDHSSPEALRGLKIEMYWDDA"
     gene            complement(340..>1124)
                     /locus_tag="PKH07_19870"
     CDS             complement(340..>1124)
                     /locus_tag="PKH07_19870"
                     /inference="COORDINATES: protein motif:HMM:NF016816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HNT37260.1"
                     /translation="YMAVDLEGISGVCVFDQTREPTTTHYQDARRLLMADINAAVEGC
                     FEGGATEIVVEDEHAGGFNFVAELIDPRVVYLTGKSRPKMSQRESIYTGFDGAILLGY
                     HSMAGTSDGILNHTQSSLRGNRYWYNGRESGEIAQAALRLGHCGVPVVMVSSDDAGCR
                     EAREFLGEDIVTVSVKRGVTIEYGFLIPPKRAHEMLQEGACKAMRLIGKREPFKMDLP
                     IQGCLRFPDKSTADAYRPVRGKRVDDHTFEAIFDRGIDVLEF"
BASE COUNT          267 a          338 c          251 g          268 t
ORIGIN      
        1 tccgcatcat cccagtacat ctcgatcttg agtcctcgaa gagcttcggg ggatgaatga
       61 tcccagaggg tcatccagat ccgtcgaata gttccgctcc ccttcacatc tgtcagaaca
      121 agtgatgaac cagatggaag tgcaacgctc ggagcacctt tgcgcccgta gtttgccttt
      181 cctgcagaac ccttctcacc tgtgacattt tcataagtga accatcgtgt ttcaaccgga
      241 ttggccggaa acttcgccaa actatcagtg atgaccatct ctgcgttctc ccaatcttga
      301 atactgtctc ggacagcaca agcacctttg cgtgcgtgtt cagaactcca aaacgtctat
      361 gcctcgatca aagatcgcct cgaaagtgtg atcatccaca cgcttgcctc gaacgggtct
      421 gtaagcatct gcagtactct tgtctggaaa tcgaagacat ccttggatcg ggagatccat
      481 tttgaatggc tctcgctttc cgataagacg catcgccttg caggctccct cctgcagcat
      541 ctcatgcgct cgtttgggtg gtatgagaaa accgtattcg atcgtcacac cgcgtttgac
      601 cgaaacagtg acaatgtctt cacccaggaa ctcacgcgcc tcgcgacatc cggcatcgtc
      661 cgaagaaacc atcacaacag ggacaccaca gtgccccaaa cgaagagctg cctgtgcaat
      721 ctctccgctt tcacgcccat tgtaccaata gcggttgccc ctgagggaac tttgcgtgtg
      781 attcaggatc ccgtcggaag tgcctgccat ggaatgatag cccagcagga tcgctccatc
      841 aaaccccgtg tagatactct cccgctggct catcttggga cggctctttc cggtaagata
      901 caccactctt ggatcgatca actcggcaac aaagttgaat ccaccggcat gttcatcttc
      961 gacaacaatt tccgttgctc caccttcgaa gcagccctca acggcggcgt tgatgtccgc
     1021 catgagaaga cggcgtgcat cctgatagtg tgtcgtcgtc ggttcacgcg tctggtcaaa
     1081 aacacaaact ccgctgattc cctccaaatc cacagccata taaa
//
LOCUS       DAOQUC010001556         1119 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_190241_length_1119_cov_3.468045, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001556 DAOQUC010000000
VERSION     DAOQUC010001556.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1119
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_190241_length_1119_cov_3.468045"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..638)
                     /locus_tag="PKH07_19875"
     CDS             complement(<1..638)
                     /locus_tag="PKH07_19875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HNT37261.1"
                     /translation="MSTTVSHKININSEMQQSYLDYAMSVIVSRALPDARDGLKPVQR
                     RILYAMYDMGIRDDTPHKKSARIVGEVLGKYHPHGDSAVYDAMARMAQDFSERYLLVD
                     GQGNFGSVDGDPPAAMRYTEARLSSPAIEILRQLDMDTVDYIDNFDGTLKEPEVLPSA
                     IPNMLVNGASGIAVGMATNIPPHNLGEVVDALVMMLENWDGIEEITVEDIMR"
     gene            complement(756..>1119)
                     /locus_tag="PKH07_19880"
     CDS             complement(756..>1119)
                     /locus_tag="PKH07_19880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008402036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37262.1"
                     /translation="ASLSTDSFLSASSFQHTIKVLAGAAIASKEDPLYGLKENVIIGK
                     LIPAGTGFVPGRFEDQVMEAEPGNPDIAVKQLDIFNEELDQVESLDDELEESEVGRFD
                     LYDDENFEDEVDLMDDDE"
BASE COUNT          248 a          313 c          254 g          304 t
ORIGIN      
        1 taccgcatga tgtcttcaac ggtgatttct tcgatgccat cccagttctc caacatcatc
       61 accagggcat ccactacctc ccccagatta tggggcggaa tgttcgttgc catgcctacc
      121 gcgatgcctg atgcgccatt cacaagcatg ttgggaatgg ccgaaggcag cacttccggt
      181 tctttcagag tcccgtcaaa attgtcaatg taatcaactg tgtccatgtc cagttggcgc
      241 aggatctcga tggctggcga tgaaagcctc gcttccgtgt aacgcatcgc cgccggcggg
      301 tcgccatcca cgctgccaaa gttcccctgc ccatctacaa gtaaataacg ttctgagaaa
      361 tcctgagcca ttcttgccat agcgtcatag accgcagagt cgccgtgcgg gtggtactta
      421 cccagaacct caccaacgat gcgtgctgac ttcttatgcg gtgtgtcatc ccggataccc
      481 atatcgtaca tggcatacaa gatacgcctt tgaactggct tcagtccatc ccgagcgtcg
      541 ggcaatgccc gcgacacgat gacactcatg gcataatcca gataggattg ctgcatttcg
      601 gagttgatat tgatcttatg ggagacggtt gtgctcataa tccgctcttt ctctggctca
      661 gtcgttgttc ttttcaattt ttaccaacta tagcataaaa agacccggag tgactgtcca
      721 cttcgggtct ttattttcaa tttctccgcg ttggactact cgtcatcgtc catcagatct
      781 acttcgtctt cgaagttttc atcatcgtag agatcaaatc taccaacttc gctttcctct
      841 aattcgtcgt ccaagctctc aacctgatcg agttcttcgt taaagatatc cagttgtttc
      901 acagcgatat ccgggttacc gggttctgct tccattacct ggtcttcaaa acgaccagga
      961 acaaagcctg tcccagccgg gatcagcttt ccgatgatga cgttctcctt gagcccatac
     1021 agtgggtctt ccttggaagc gatagccgcc ccagccaata ccttgatggt gtgttggaag
     1081 gaggatgccg acaggaacga atctgtgctg agtgatgcc
//
LOCUS       DAOQUC010001557         1117 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_190967_length_1117_cov_2.083804, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001557 DAOQUC010000000
VERSION     DAOQUC010001557.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1117
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_190967_length_1117_cov_2.083804"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..416
                     /locus_tag="PKH07_19885"
     CDS             <1..416
                     /locus_tag="PKH07_19885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37263.1"
                     /translation="KNQVNLWVYLAFGKEECEKKMKVIAESYPDEATLKKAYRHLQRV
                     SQNGVLNVAHALDEWRRAGFADMSLQAALQIFEELSLTFLVDQGTLVRLADSPEKRSL
                     EESSTYCYARLQRESFEQWSRWLLTSPSPEVARKL"
     gene            413..>758
                     /locus_tag="PKH07_19890"
     CDS             413..>758
                     /locus_tag="PKH07_19890"
                     /inference="COORDINATES: protein motif:HMM:NF014047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HNT37264.1"
                     /translation="MTTNSTNCVVRIQGLSSAGQGVARAEDGKVVFIEGALPDETCLI
                     RRHYHKRTFDRGTLLEIVTPSPDRIEPRCPHYSECGGCQLQHLRYSEQLVFKHTRIRE
                     AFSRIAGFSDVPI"
     assembly_gap    759..768
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <769..>1117
                     /locus_tag="PKH07_19895"
     CDS             <769..>1117
                     /locus_tag="PKH07_19895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37265.1"
                     /translation="WEYRNKLALTLEGTDKSSLMGMMKSASHETVEISDCLIASPAIR
                     KVIRPLKQFQQKLTPFFEKIHIRLLGDSLVLVFDARRKLTNLDVAESAASKAAQGLGA
                     QGFCINSLGSALDP"
BASE COUNT          271 a          289 c          290 g          257 t
ORIGIN      
        1 gcaagaacca ggtcaatctc tgggtctacc tcgcgttcgg aaaagaagag tgcgagaaaa
       61 agatgaaggt catcgcagaa tcctatccgg acgaggcaac gctcaaaaag gcctaccgtc
      121 atcttcaacg ggtgtcccag aatggggttc tgaatgtcgc ccatgcattg gatgagtgga
      181 ggagagcagg ctttgcggat atgagccttc aggccgctct tcagatattt gaggaactat
      241 cccttacctt cttggtagat caaggcacac tcgtgcgtct ggctgattca cctgaaaagc
      301 gttcattgga agagtcttcc acatattgct atgcgagact ccaacgcgag tcttttgaac
      361 aatggtcaag gtggcttctg acatctcctt cgccagaggt ggcacgaaaa ctgtgacgac
      421 aaactcgacc aattgtgtcg ttcgtatcca aggactttcc tcggctggac aaggtgtcgc
      481 tcgagccgag gatggtaagg tcgtcttcat cgaaggtgca ctgcccgacg aaacctgcct
      541 catacgcagg cactatcaca agaggacgtt tgatcgaggt acacttctcg agatagtcac
      601 tccttcccct gaccggatcg agccccgctg ccctcactac tctgaatgcg gcggctgcca
      661 acttcagcat ttgagatact cagagcagct ggtgttcaag cacaccagga ttcgagaggc
      721 cttcagccga atcgcgggtt tcagtgacgt ccccattcnn nnnnnnnngt gggaatatcg
      781 gaacaagctg gcactcactc tggaaggtac cgacaagtca agtctaatgg ggatgatgaa
      841 gtctgcttcc cacgagacgg tcgaaatctc cgattgcctt attgcgtctc cagcgattcg
      901 caaggtcatt cgccccttga aacagtttca gcaaaaactt accccattct ttgaaaagat
      961 acacatacga ctgctcggcg acagtctggt tctggtattc gacgccagga gaaagttgac
     1021 aaaccttgat gtggcggaat ctgcggcgtc gaaagcggct cagggtcttg gcgctcaagg
     1081 attctgcatc aacagccttg ggtcagcttt ggatccg
//
LOCUS       DAOQUC010001558         1116 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_191171_length_1116_cov_2.707823, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001558 DAOQUC010000000
VERSION     DAOQUC010001558.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1116
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_191171_length_1116_cov_2.707823"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..971)
                     /locus_tag="PKH07_19900"
     CDS             complement(<1..971)
                     /locus_tag="PKH07_19900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015942713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HNT37266.1"
                     /translation="MGKRQDIDKVMIIGSGPIVIGQACEFDYSGTQACKALRRLGYKI
                     VLANSNPATIMTDPGMADFTYIEPLNVKTMEAIIEKERPDALLPNLGGQSGLNLCSEL
                     AKAGVLDKYGVEVIGVKADAIERGEDRLAFKQTMEKLGIEMPKSEIAYSVEEAEKIAS
                     VLGYPVVIRPAYTLGGTGGGLVYNVDELRLVGSRGLSASLVGQILVEESVLGWEELEL
                     EVVRDAKNQMITVCFIENVDAMGVHTGDSFCTAPMLTIDEALQKKLQKYSYDIVESIE
                     VIGGTNIQFAHDPKTDRVVVIEINPRTSRSSALASKATGFPIAYVSSL"
BASE COUNT          277 a          320 c          241 g          278 t
ORIGIN      
        1 agtgacgaaa cataggcaat cgggaacccc gtcgccttgg aggcaagcgc tgacgaacgt
       61 gaggttcttg ggttgatctc gatcaccaca acacgatcag tctttggatc atgcgcaaac
      121 tggatgtttg tcccaccgat gacctcgatt gattccacaa tgtcatagga gtatttctgc
      181 agtttcttct gaagcgcttc gtcaatggtc aacataggcg ctgtgcagaa cgagtcacca
      241 gtgtgtacgc ccatcgcatc tacgttctca ataaagcaga cggtgatcat ctggttcttg
      301 gcatcgcgaa caacttcgag ttcaagttct tcccaaccca aaacagattc ctcgaccagg
      361 atttgtccta caaggcttgc agaaagtccc cggctgccaa ccaggcgaag ttcatccacg
      421 ttgtagacca gaccaccacc cgtcccaccc aacgtgtatg caggacgaat aactaccgga
      481 tagccaagta cagaggctat cttctcggcc tcttcaactg aataggcgat ctcgctcttg
      541 ggcatctcaa tgcccagttt ctccatggtc tgcttgaaag caagcctgtc ctcgccacgt
      601 tcaattgcgt ccgcctttac accgataact tcaactccgt acttatcgag cactcctgct
      661 tttgcgagtt cagaacacag attcagaccc gactgcccac caagattcgg cagaagagca
      721 tcggggcgtt ccttttcaat aatcgcctcc atggtcttta cattcagagg ttcgatgtag
      781 gtgaaatccg ccatgcctgg atcggtcatg attgtggccg gatttgaatt tgcaagaaca
      841 atcttgtacc caagtctccg aagcgccttg cacgcttggg ttccggaata gtcaaactca
      901 cacgcttgac cgataacaat aggaccggaa ccgatgatca tcaccttgtc aatatcttgc
      961 cttttgccca tcttgcctac acctctcctc tgaatttgga cgatctctca aacctgcaat
     1021 ccctgagtaa cttgactggg aatctcagtc tttccgaatg cacaaactcc cgcacaacag
     1081 ggatctccct gaaaccagag caatgcattc ttaggg
//
LOCUS       DAOQUC010001559         1114 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_191767_length_1114_cov_2.954674, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001559 DAOQUC010000000
VERSION     DAOQUC010001559.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1114
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_191767_length_1114_cov_2.954674"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..651)
                     /locus_tag="PKH07_19905"
     CDS             complement(<1..651)
                     /locus_tag="PKH07_19905"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HNT37267.1"
                     /translation="MAVLRSNWLYALQRLSFSPWFPVLLGILCGCFVGLLLSVGALDS
                     YEYVLYDWRFNQRAPIAPSRSVNVVYINDATLKNMADSLSRWPWSRNVWAAFQQEILN
                     PIQAKTVVYDIVFDLPDSEPEADQAFASVIRNSKNILMTSLFRDAREGFISLAERQAY
                     QEGWQQPPEEMQRLALGVDLSQRRWEVRYVPDYMTTDLPLSDFLTAAAGSGATTYEP"
     gene            complement(639..>1114)
                     /locus_tag="PKH07_19910"
     CDS             complement(639..>1114)
                     /locus_tag="PKH07_19910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37268.1"
                     /translation="DQDARTAEGRKMEGVDRQDRLPEVGAQVTGSLTSMTAVRVGYQF
                     AENRSSGAFYRHVSHQVYGMVFQELTEKLSAYAYLERQWKFYEKQIAYSDPLGTLSPN
                     GIRDDRRWLALFGLQYQFCPRLSLVGEYSRISNHSNDDTSPYRGNQYSLGMIWQF"
BASE COUNT          263 a          297 c          300 g          254 t
ORIGIN      
        1 aggctcgtag gtggtggcac ccgagcccgc tgcggcggtc aggaaatccg acaggggcaa
       61 atccgtggtc atgtagtccg ggacataccg gacttcccat cgtctctgac tcagatcaac
      121 tccaagtgcc aggcgctgca tttcctccgg tggttgctgc cagccttcct ggtatgcctg
      181 gcgttccgcg agtgatatga atccttcccg tgcgtctcga aagaggctgg tcatcaggat
      241 attctttgag tttcggatga ccgaagcaaa cgcctgatcg gcctctggct ctgagtcggg
      301 aaggtcgaaa acaatatcat agaccacagt cttggcctgg atgggattca atatctcctg
      361 ctgaaaggcc gcccaaacat ttcggctcca aggccagcgc gaaagtgagt ctgccatgtt
      421 cttcaaagtt gcatcattga tgtagaccac attgacggat ctgcttgggg caatcggcgc
      481 tctctggtta aaacgccagt catagaggac atactcgtaa gagtcgagag caccaacgga
      541 gagaagcagt ccaacaaaac aaccgcagag tatacctaac agtacaggaa accaaggtga
      601 gaaactcagc ctttggagcg catagagcca gttcgatctc agaactgcca tatcattccc
      661 agcgagtatt gattgcccct gtaggggctg gtgtcatcgt tggagtgatt cgagattcgg
      721 ctgtactcgc cgacaagtga caagcgcgga cagaattgat actgcagacc gaacagcgcc
      781 agccaacgcc ggtcgtctcg gattccattc gggctgagag ttcccagagg atccgagtag
      841 gcgatctgtt tctcatagaa cttccattgg cgctcaaggt atgcataggc ggagagtttt
      901 tctgtcaact cctgaaatac catcccatag acctgatggc tcacatgtct atagaatgct
      961 ccagaagagc gattttccgc gaactggtag ccgactcgaa ccgcggtcat tgaggtgaga
     1021 gagcctgtga cctgagcacc aacctcgggc agacgatctt gtcggtcaac tccttccatc
     1081 tttcggccct ctgctgttcg agcgtcttgg tcgt
//
LOCUS       DAOQUC010001560         1114 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_191774_length_1114_cov_2.826251, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001560 DAOQUC010000000
VERSION     DAOQUC010001560.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1114
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_191774_length_1114_cov_2.826251"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            458..>1114
                     /locus_tag="PKH07_19915"
     CDS             458..>1114
                     /locus_tag="PKH07_19915"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HNT37269.1"
                     /translation="MDRSLKCLVVFKSLYLVIMLLFGMGPHVGHAAWYDTYDFSGLGV
                     TGFYPLDGNANDVSGNGLNGTAYNCSSATGHFAECYDFNGVMGSSGSRVWLPPFPHCE
                     ALSIAAWVYMEEYAPGTTYLEHYTIFLRRQDYADKSLMIDKNGYLHLAEIRGSGNPSS
                     VVQSTSTVPLNGWHHVAGTLDQNYIRVYIDGILAGESASAGPLYWGGSYYCTHIGMGS
                     D"
BASE COUNT          271 a          232 c          331 g          280 t
ORIGIN      
        1 ccagttcatc ttctcaaccc tccattccat gagcatggta ggaagttcac gggggtaagt
       61 caagcgagga tgggtgaaac ccatcagttt gggaagactc agttggtggt tcgagactcc
      121 atctccactc gggagaaggg tggggatgag ggcaaaggag tgggggcatc ttgcccccgt
      181 cgaaatcggt gtggggcgag acgccccagc tcctgcggaa agggtaagaa tgtcagagaa
      241 gattggcagg aagggtgaaa gccatcagtc gagtgagact taactggtgg gtcacgaccc
      301 accctacagg cttagatgaa tattgagaaa ggagcgtgga ggggatgaga gaaagtactt
      361 cgtaaaggat tgtgatgagc agtttttgac aactttgttg atcctgagga acacgatctg
      421 gttttccagg tctgaagaca cgaagaggag gaatatcgtg gatcgatcat tgaaatgtct
      481 tgttgtcttc aagagtctgt atctggtcat tatgctgctg tttggtatgg gtccgcatgt
      541 gggacatgcc gcatggtatg acacatacga cttcagcggg cttggggtga ctggattcta
      601 tccccttgat gggaatgcaa acgacgtgag tgggaatggt ttgaatggca cggcttacaa
      661 ctgctcatca gcaactgggc atttcgccga atgctatgat ttcaatggag tcatgggatc
      721 ttccggttca agagtatggc ttccgccttt ccctcactgt gaggcgttat ccatagccgc
      781 ttgggtgtac atggaagagt acgcccccgg aacaacatat ctggaacatt atacgatatt
      841 cttgagaagg caggactacg cagacaaatc tttgatgata gacaagaatg gttatcttca
      901 tctcgccgag attcgcggtt cggggaatcc gtcaagcgtt gtacaaagca catcaactgt
      961 gccgctgaac ggctggcatc atgtagctgg aacgctggat cagaattata tccgtgtcta
     1021 tattgatggt atcctcgctg gggagtctgc ttccgctggc ccgctgtact ggggaggttc
     1081 ttactattgc acccacatcg ggatgggtag tgat
//
LOCUS       DAOQUC010001561         1114 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_191790_length_1114_cov_2.703494, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001561 DAOQUC010000000
VERSION     DAOQUC010001561.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1114
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_191790_length_1114_cov_2.703494"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..239
                     /locus_tag="PKH07_19920"
     CDS             <1..239
                     /locus_tag="PKH07_19920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37270.1"
                     /translation="STARILVLAIMTSLSVLNGIAEDKANITEPQPYQLWACIGETQE
                     DVLKKCGEPKWIPFFDYATVGEYRNGDPRCRCYF"
     gene            complement(784..1008)
                     /locus_tag="PKH07_19925"
     CDS             complement(784..1008)
                     /locus_tag="PKH07_19925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37271.1"
                     /translation="MREKTLVDEVVDWLTGKGNYDHDEYVTQPPENCVLNVAGSRESK
                     ADGIQDLVMAIMVDVLVRGNPECGKYYPLA"
BASE COUNT          304 a          281 c          260 g          269 t
ORIGIN      
        1 gatcaacggc gagaatctta gtgctggcga tcatgaccag cctgtcagtc ctgaatggca
       61 ttgcggaaga caaagccaac atcacagaac cgcaacctta ccagttatgg gcttgcattg
      121 gtgaaaccca agaagacgtg ctcaagaaat gcggcgagcc aaagtggatt ccattcttcg
      181 actatgcaac ggttggtgaa tatcgaaacg gggacccacg ttgcagatgt tatttttaac
      241 gggacaacgc cttgtccatc tggaaaacag cataataccg cttgccagaa aatatgatgt
      301 gcgcaggttt cggatcgtcg atagtcactg aaccaaggga aaagaagaaa gcgatttcct
      361 tggggatgcc ggttccatga tgcaggaacg aggacttgtc catctgctta acagcaacag
      421 tatctgacaa aacctcccac gaataggctt ataaagcttg gcatgcctca cctccctcag
      481 ccaattctcg cttcgttctc ttcacatacc agtctaggct caagacgagc aacatgatat
      541 aagacaatgt ctcaaatttt ctctcaagca atggtcacga gtcctaagat tatgggaaaa
      601 caccccagta agacgtgttg cccggaagag cactgaagac gctcttgatt gctgtatctg
      661 tctgggtgta ataagagtca aagacagatg aaagcttttg gacaatcgtg actacatcag
      721 gtttactgag cgttggcttt attttgcacc ataggttact tcagttttgc cttcctccgc
      781 ctcctacgcc aacggataat acttcccgca ctcaggattc ccccggacaa gaacatcgac
      841 catgatggcc atgacaagat cctgaatccc gtcggccttg gactccctgc tgccagccac
      901 attcaaaaca cagttctcag gcggctgtgt gacatactcg tcatgatcgt aattgccttt
      961 accagtgagc cagtcaacga cttcatcgac gagcgttttc tccctcaacg cccactcgga
     1021 ggtttacacg taaactacgg cggcgtatat tcattggcgt gaaaggcaag atgcattttt
     1081 gagttttgcg cgcaaggtaa ggtggcaaca gggg
//
LOCUS       DAOQUC010001562         1113 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_192287_length_1113_cov_1.614367, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001562 DAOQUC010000000
VERSION     DAOQUC010001562.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1113
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_192287_length_1113_cov_1.614367"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(182..493)
                     /locus_tag="PKH07_19930"
     CDS             complement(182..493)
                     /locus_tag="PKH07_19930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HNT37272.1"
                     /translation="MKILDEIRGYIMENILFGDEPKLEMDTLFHEGGILDSTGFLEII
                     TFVEERFEIKIADSEVIPENLGTLRRISNFVETKLREKTAVARLERQVPASVGNTPCG
                     G"
     gene            complement(536..>1113)
                     /locus_tag="PKH07_19935"
     CDS             complement(536..>1113)
                     /locus_tag="PKH07_19935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37273.1"
                     /translation="GLAHALIGRNLDPESKIIEFTRVMESGLGEKVDIAFFKFCFVDF
                     GQTSNPEAIFTSYCNAMDSLNARFPRVTFMHVTVPLCGPAKKAKGMLKASVKRLIGRP
                     PVLAENLVRSRYNALLRERFSGKEPLFDLALYESTDLEGLQHYGLRSGQEVPLLVCSY
                     TDDGGHLNATGRRHVAEQLLIQLANLPGGVP"
BASE COUNT          230 a          352 c          283 g          248 t
ORIGIN      
        1 agttgtcgca tagtcgagac ggtttgacga ccacgaatcg ggcgactacg ttgctcatga
       61 tctgatgcat ctcggtggag gccccagtcc aatcgtcgag gtagacgctc gtctcatcac
      121 gaccccggcg ccccggcccg ccaaccgacg gcacccttcg ttcccggcct ccagatgccg
      181 cctatccccc acatggagtg tttccaacag aggcggggac ctggcgctcc agtcgtgcga
      241 ctgctgtctt ctctcgcagc tttgtctcca caaaattgga tatcctgcgc agtgtgccga
      301 gattctcggg gatgacttcg ctgtccgcaa tcttgatctc gaacctctcc tccacgaatg
      361 tgatgatctc cagaaaaccg gtcgaatcga gaatgccccc ttcgtggaac aacgtgtcca
      421 tctctagttt cggttcatcc ccaaaaagga tgttctccat gatgtaccca cgaatttcgt
      481 ccaggatctt catagtaccc cgcttccgcc aatctacata gtactgttcg ccccatcacg
      541 gcacgccgcc tgggagattc gccaactgga tcaacaactg ctccgccaca tgtcgtcggc
      601 ctgtcgcgtt gagatgacct ccgtcgtccg tgtatgaaca aacaagaagt gggacttcct
      661 ggccactcct gagaccatag tgctgcagcc cctccaggtc ggtactctcg tacaacgcca
      721 agtcaaacag aggctccttt ccggagaacc gctcccgcaa gagggcattg tatcgagatc
      781 gcaccaggtt ctccgccaga acaggcggcc ggccgatgag tcgtttgacg cttgccttca
      841 gcattccttt ggccttcttg gccggcccac acaaaggcac tgtcacgtgc atgaatgtca
      901 cgcgaggaaa gcgggcgttc agcgaatcca tggcgttgca gtatgaggtg aagatcgctt
      961 ccggattcga ggtttggccg aaatccacga agcagaactt gaagaatgcg atgtctactt
     1021 tctctcccag cccgctttcc atgaccctgg tgaactcgat gatcttcgac tccgggtcga
     1081 gattgcggcc gatcagcgca tgggccaacc ccg
//
LOCUS       DAOQUC010001563         1112 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_192372_length_1112_cov_3.145695, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001563 DAOQUC010000000
VERSION     DAOQUC010001563.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1112
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_192372_length_1112_cov_3.145695"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..472
                     /locus_tag="PKH07_19940"
     CDS             <1..472
                     /locus_tag="PKH07_19940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37274.1"
                     /translation="VKDGYYEKQMCQQGGLCMADERPGDDNVWGQDPDKVVLPNQPYR
                     LDFVMLPAAQVVVHLKNDEGKPIPNQRIEVYGPGYPSTSVFAQVDTDETGTARIANVP
                     LKKYDFTLRLNKEGSTCSPVALEFSEPVLYEIDMVFVQGQNDSNALEVKVLSSP"
     gene            648..>1112
                     /locus_tag="PKH07_19945"
     CDS             648..>1112
                     /locus_tag="PKH07_19945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008860226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37275.1"
                     /translation="MKKKRALISVYDKTGVVEFATGLHKLGWEILSTGGTSKALKEAG
                     IPVLSVSDVGQFPEILDGRVKTLNPRIFGGILARQNDPKHMKELDEQGIVPLGLIVVN
                     LYPFEATIAKPDVTLAEAQEKIDIGGVSLLRAAAKNYPSVAVVCDPEDYSEVL"
BASE COUNT          258 a          273 c          315 g          266 t
ORIGIN      
        1 cgtcaaggat ggttactatg aaaagcagat gtgccagcag ggtggtctct gcatggcgga
       61 cgaacgccca ggcgatgaca atgtctgggg acaggatccg gataaggttg tcctgccgaa
      121 tcagccctat cgtttggatt ttgtcatgct gccggcggcg caggtcgtcg tccacttgaa
      181 gaacgatgaa ggcaagccaa ttccgaatca gcgcatcgaa gtgtatgggc ctggatatcc
      241 ttctacgtcg gtctttgcgc aagtggatac cgacgaaacg ggcacggcac gaattgcgaa
      301 cgttccgctc aagaaatatg acttcacgct gaggctcaac aaagaaggct cgacttgcag
      361 tcccgtcgct ctggagttta gcgagccggt gttgtacgaa attgacatgg tattcgtgca
      421 gggtcaaaac gactcgaatg cgcttgaggt caaggtgctt tcttcgccat agccttctct
      481 ctcgtacgcc ctgcttcggc agcacacaga gccgtgctac cgaagtatgc gaaggcggga
      541 ctttcttttc ttcgaatcct tctcttgctt tcctgatgcc aaggatacta tagtactgca
      601 ttcttaaaga atgagttaat cctttgggag gaggtttcat cttggagatt aagaaaaagc
      661 gtgctttaat cagtgtttat gacaagaccg gtgtggtgga atttgccacg gggttgcaca
      721 aactgggttg ggagattctc tccaccggtg gcacatccaa ggcactcaag gaagcgggaa
      781 tacccgttct gagcgtgtca gatgtggggc aatttccgga aatcctcgac gggcgcgtca
      841 agacgctaaa cccaagaatc tttgggggca ttctcgcccg ccaaaacgat cccaagcaca
      901 tgaaagaact cgatgagcag ggcattgtgc cgttgggtct cattgttgtg aatctctatc
      961 cgtttgaggc gacgattgcc aaaccggacg tgacgctcgc agaagcccag gagaagatag
     1021 acatcggtgg cgtgtcgctc ttgcgggctg ctgccaagaa ctatccttcc gtcgcggtcg
     1081 tctgtgatcc cgaggattac tccgaagtgc tc
//
LOCUS       DAOQUC010001564         1111 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_192637_length_1111_cov_4.232008, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001564 DAOQUC010000000
VERSION     DAOQUC010001564.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1111
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_192637_length_1111_cov_4.232008"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..561
                     /locus_tag="PKH07_19950"
     CDS             <1..561
                     /locus_tag="PKH07_19950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37276.1"
                     /translation="WEKQSDYCRRLFQDIVSTSKAVSPEMRVCISPFFILDRNRILGD
                     FRFAEPAEYAQWWQTTLKQTGITWLMLQDSGEHASFTSVAEKEPFIAAYSEACKRSGV
                     EFWLNVEIAEHDVPDYQTQRLDRDKPFNEQKWRAVPEERLREKLALASKFSPSAVSWG
                     WDWWRPTSEQSSPERYAAFQNLNRGT"
     gene            complement(632..>1111)
                     /locus_tag="PKH07_19955"
     CDS             complement(632..>1111)
                     /locus_tag="PKH07_19955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthetase B"
                     /protein_id="HNT37277.1"
                     /translation="DYSKCLAFQNFTLYTDPMVYEYADIDIHPNMVDQILPKDEHFVL
                     FEFSAKLDPVPSMLTQCHTARIEEFRGQTTAFWKDKVKNRVVIMAEFPGRNLVKYLHG
                     NWGQGTFTYFAGHDPEDVEHLVGEDPTDLSLHKNSPGYRLILNNILFPAARKKEKKT"
BASE COUNT          252 a          276 c          308 g          275 t
ORIGIN      
        1 tgggaaaagc aaagcgatta ttgccgccga ctcttccagg atattgtctc gacgtccaag
       61 gcggtctctc ctgagatgcg cgtttgcatc tcgccgtttt tcattctgga tcggaacagg
      121 atcctgggtg acttccgatt tgccgagccg gccgaatatg cgcaatggtg gcagacaacg
      181 ctcaagcaga cgggaatcac ctggctgatg cttcaagact ctggagagca cgcttccttc
      241 accagcgttg cagagaagga gccgttcatc gccgcatact cggaagcctg caaacgcagt
      301 ggagtggagt tctggctcaa tgtggaaatt gccgagcatg acgttccgga ctatcagaca
      361 caacgccttg acagagacaa gcccttcaac gagcagaaat ggcgtgccgt gcctgaagag
      421 cggctgagag agaaacttgc ccttgcatcc aagttctcgc cgtcggctgt ctcatggggt
      481 tgggactggt ggcgtccgac gtcagagcag agttcccccg aacggtatgc agcgtttcaa
      541 aatctgaacc gtgggacttg atgtctgtcg atacggcttt tccatgcttc gataacagat
      601 gctttgcggc gctgccgaat ctgcgatccg tctaggtctt cttctctttc tttcttgccg
      661 caggaaagag gatattgttg aggatcaacc ggtagccagg ggagttcttg tgaaggctca
      721 gatcggtggg atcttcaccg acgagatgct caacgtcttc gggatcatgt ccggcaaaat
      781 aggtgaatgt accctgaccc cagttgccat gaaggtattt gacgagattg cgaccgggga
      841 actctgccat aataacgact cgattcttga ccttgtcctt ccagaaggcg gtcgtttgtc
      901 ctctgaattc ctcaattcgt gccgtgtggc attgcgtcaa catgctggga accggatcga
      961 gttttgccga gaactcgaag agtacgaaat gctcatcctt ggggagaatt tgatccacca
     1021 tgttggggtg aatgtcaata tccgcgtatt catagaccat cggatcggtg tatagcgtaa
     1081 aattctggaa tgcaagacac ttgctatagt c
//
LOCUS       DAOQUC010001565         1109 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_193218_length_1109_cov_3.520873, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001565 DAOQUC010000000
VERSION     DAOQUC010001565.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1109)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1109)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1109
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_193218_length_1109_cov_3.520873"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            465..>1109
                     /locus_tag="PKH07_19960"
     CDS             465..>1109
                     /locus_tag="PKH07_19960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37278.1"
                     /translation="MMKSLLSRMFCILFVGLVLGGTLISAAGADEIPVAIARDGSVVL
                     KSLDTHQGLSIVNTATGKEIKISDAPGAGYFASLSPDAKKVCFKMITVEPDGSRSFVP
                     AIYSVATGKTIALSEPSVDAGTPVVSADGNIAFTVGKTLTVLDAKMNVLGTFDLGHAV
                     NLLGLSPDGSQIAYNNKEEQIALCNLTSGESRVLTDGSGAFWNPQFSPDGNSLLV"
BASE COUNT          237 a          268 c          304 g          300 t
ORIGIN      
        1 gtctctttcg cagaatcggt tagtcgtgtt ggctcaattc tgcaggtgct atggccagga
       61 tatgcttcgg agcagcgcta tcgaagccgc tgtctttctg ttcccgcttc ggcagcgtcg
      121 cagagcctat gcaactgaag catacctcca ccctctccgc aacagcgcta atacttcctc
      181 accatgaaaa gtcattgaca acgtcccaag agggggcgta acatcctaga tactgatttg
      241 taatgaagac ctgcgtgaat ggcctgttag ggcggttctt ttatgtgctt gggaagcggt
      301 ttcttgatgt gtaagaattc ttcagggaga gacacatgct ggcatgtaag tgtcgcctgt
      361 gttcataatc acccgtatcg gaagaaggga gggatccact gccggactct ggttctcgtt
      421 gcgtaggttt gggcattgtg tatgtttcga agaaaggaaa tccgatgatg aagtctttgc
      481 tttctcgaat gttttgcatt ctgtttgttg gtctggtctt ggggggaact ctgatttccg
      541 cagcgggcgc cgatgagatt cccgttgcca tcgctcgaga tggttccgtt gttttgaagt
      601 ccctggatac ccatcagggg ctgtcaattg tcaacaccgc tactggtaaa gagatcaaga
      661 tttcggatgc tcctggggca ggatacttcg cgtcgctttc tcctgacgcc aagaaggtct
      721 gcttcaagat gatcaccgtt gagccggacg gaagtcgctc gtttgttcct gcgatttaca
      781 gtgtcgcgac aggaaagacg attgcgttgt ccgaaccaag tgtggacgct ggaacaccgg
      841 ttgtctcagc cgatggcaac atcgccttta ctgtcggaaa gactctgacg gtgcttgacg
      901 ccaagatgaa cgttctgggt accttcgatc taggtcatgc ggtgaatctg ttggggctct
      961 cgcccgacgg ttctcaaatc gcctacaaca acaaagaaga acagattgcc ctctgcaatc
     1021 tgacgtctgg agaaagccga gttctaactg acggcagtgg tgccttctgg aatcctcagt
     1081 tctccccaga tggaaactca ctgttggtg
//
LOCUS       DAOQUC010001566         1109 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_193304_length_1109_cov_2.464896, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001566 DAOQUC010000000
VERSION     DAOQUC010001566.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1109)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1109)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1109
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_193304_length_1109_cov_2.464896"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(152..>544)
                     /locus_tag="PKH07_19965"
     CDS             complement(152..>544)
                     /locus_tag="PKH07_19965"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNT37279.1"
                     /translation="PPTSFQAESFDLVLGLSIFTHLNEQDQFAWLSELRRLSCKGALI
                     LVSVHGEPAICRLGEPSALWTYRASSGFLDLGANHDLKGILPESSYRSIYHSPTYIRD
                     NWSRYFTVLRVIPGYFWGYQDLVVLRND"
     assembly_gap    545..554
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<555..1047)
                     /locus_tag="PKH07_19970"
     CDS             complement(<555..1047)
                     /locus_tag="PKH07_19970"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNT37280.1"
                     /translation="MNGVAFDQVEYPLECEPVRKAFWYVPQAGDCVFRCRTPFNPNRD
                     LSDPFVEIQFVRKDSNVPFRPSQASYYCFGDHEPLPPLELRRRVHRGDDAFSFNSVGA
                     TTFMQFQQVLKSLGFSGYQAFAHILDWGCGCGRSTRYFRHIRGASVTGIDIDAEALAW
                     CREN"
BASE COUNT          303 a          290 c          287 g          219 t
ORIGIN      
        1 cagtcaaagg cgaccagaaa tgaacacgcg ttcagccagc tgcctacagt ctgattgcac
       61 aggcagaggt ctgtcaaacg cctgtgagag caagaagcac tcttgtgcgc ttcgttgaga
      121 tggtggcggt gtcgctgaag cgagttgctg gtcaatcgtt gcgaagaacc accaggtctt
      181 gatatcccca gaagtaacct ggaatcacac gtagaacggt gaaatagcga gaccagttgt
      241 ctcgaatgta tgtcggcgag tgatagatgc tgcggtaaga ggactctggc aggatgccct
      301 tcagatcatg attggcaccg aggtcgagaa aaccacttga tgcccggtac gtccagagtg
      361 ctgaaggttc acccaaccga cagattgccg gttccccgtg aacactgaca aggatcaatg
      421 cacccttgca cgacaaacga cgcagttccg aaagccaggc aaactgatcc tgctcattga
      481 gatgagtgaa gatcgagaga ccgagaacca aatcaaaaga ctccgcttgg aaagatgtcg
      541 gtggnnnnnn nnnnggttct cccgacacca ggcaagcgcc tcggcatcaa tatctattcc
      601 cgtaaccgaa gcacccctta tgtgacggaa ataacgtgtc gagcgtccac aaccgcaacc
      661 ccagtccagt atatgagcga atgcctggta gcctgagaaa cctaggctct tgaggacttg
      721 ctgaaactgc atgaaagttg tcgcccccac cgagttgaaa gagaacgcat cgtctccgcg
      781 atgaactctc cttctgagtt ccaacggagg caaaggctca tgatcgccga agcaatagta
      841 ggatgcctgg ctgggtcgga agggaacatt tgaatctttg cgaacaaact gaatctcgac
      901 aaatgggtcg gacagatctc gattcgggtt gaacggagtt cggcatcgaa acacgcaatc
      961 tcccgcctga ggaacatacc agaaagcctt tcttaccggc tcacactcaa gagggtattc
     1021 gacttgatca aacgcaacgc cgttcaaggt gaaactgact cccgatactt cctcctgagg
     1081 cggaaaagcc catccctcga cttccagaa
//
LOCUS       DAOQUC010001567         1109 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_193359_length_1109_cov_2.115750, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001567 DAOQUC010000000
VERSION     DAOQUC010001567.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1109)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1109)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1109
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_193359_length_1109_cov_2.115750"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..403
                     /locus_tag="PKH07_19975"
     CDS             <1..403
                     /locus_tag="PKH07_19975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37281.1"
                     /translation="GAYANNARSALAPAADGTAITVNFMNSDAEGMSNTSYTDYYVDY
                     YQDGAYVCLANATRFSRLDNATSNTTWVAATQFSGTIGYKEDTATDYTANQAALIVRL
                     VGANGLPYPAGSRVDNIVVDDGSPVEDWYLY"
     gene            578..>1109
                     /locus_tag="PKH07_19980"
     CDS             578..>1109
                     /locus_tag="PKH07_19980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37282.1"
                     /translation="MFSRGKRGFVSICPIVVFISICLAGCRPSESEHIAQWQERFVSR
                     DELRVYVQEFRVSDLTPRWEYNSQGGAKRAIADMMAEQWLAAKAMDSGLDKDSSVQAQ
                     LAFHDHKELATHCVRKLLQQDDFSEEDLKEYYEQHSEEFHQEETVKFQDLFVRVVSKE
                     EEQSARTKIESLHQRAL"
BASE COUNT          270 a          265 c          306 g          268 t
ORIGIN      
        1 gggtgcctat gccaacaacg cacgtagtgc tttggctcct gcggcagacg gtactgcgat
       61 tacggtcaac ttcatgaaca gtgatgcgga gggtatgagc aacacctcct acactgatta
      121 ctatgttgac tactatcagg acggtgctta cgtttgtttg gctaatgcca cccgtttcag
      181 caggttggat aacgcgacgt cgaacacaac atgggtagcg gcaacgcagt tctccggcac
      241 gatcggatac aaagaggata ctgccactga ttacacagcg aatcaggcag ccctcatcgt
      301 tcgtctcgtg ggcgctaacg gtcttcccta tccggctggt tcgcgtgttg ataacattgt
      361 tgttgacgat ggcagcccgg tcgaagactg gtatctctac tgagattcca ctttgagcga
      421 agatctttga tgaagagcgt cgagaaatcg gcgctctttt ctattcgtgg gctgacgttc
      481 cagtgtccaa gcggcctgaa tggaaccttt tgacaaggat ttctggagca tgatacgttc
      541 actgcgagag tgaaatctgt gcagtttgga gatgccgatg ttttccagag gtaagagggg
      601 atttgtcagt atatgcccta ttgttgtttt catttctatc tgtcttgccg gatgccgccc
      661 gagcgaatct gaacacattg cacagtggca ggaacggttc gtgtcccgcg acgaattgag
      721 ggtctacgtt caggagttcc gagtctcgga tctcacgccg cgctgggagt acaactccca
      781 ggggggtgcc aaaagagcca ttgccgacat gatggcagag cagtggctgg cagccaaggc
      841 gatggactca ggtctggaca aggattcatc tgtgcaggca caactggcat ttcatgacca
      901 taaggaactt gccacccatt gcgtcagaaa gctgctgcag caggatgatt tctccgaaga
      961 agatctgaag gaatactacg agcagcattc ggaggaattt caccaggaag aaacggtgaa
     1021 gtttcaagac ctctttgtcc gagttgtttc aaaagaagaa gagcaatccg cccgcaccaa
     1081 gatcgaatct ctgcatcaga gagcactga
//
LOCUS       DAOQUC010001568         1108 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_193488_length_1108_cov_3.422602, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001568 DAOQUC010000000
VERSION     DAOQUC010001568.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1108
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_193488_length_1108_cov_3.422602"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1108
                     /locus_tag="PKH07_19985"
     CDS             <1..>1108
                     /locus_tag="PKH07_19985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37283.1"
                     /translation="WVQVEVATQSGQTYTAREHFTVLSVDLAVDSDNNGIIEPADDAV
                     ETLNPGNLICVNRDDDNINDLQDRDEDEDFVYGEDELSPVILTWQGPRVGVIRLSLLD
                     TEMSERSAVYTNSFKGDINRLDLPVEWNLRNCTPPGVLYIEGVDRVRNDLHVAWKVDN
                     QTICEDVIRFTVFDMNIAMDGNRDGTIDFNNPEDDKYVFWVNDDRDVRYHDESPDYMC
                     WVEDDLQGGTSDCDDDRIGTDTSPFVRGCRRDLEDFTRVHLFVDDVAVSCENLNFYLR
                     FKRGTPSPTVNLFSAVDGGSTRYLTNPEKADEQKEETRLLTVTSSEIQLPSGYPKTGG
                     APFHFILEGKSPGKGQFCLIAKAGEDHRAEAIVDL"
BASE COUNT          290 a          253 c          324 g          241 t
ORIGIN      
        1 gttgggttca ggtcgaagtg gcaactcaga gtgggcagac atacacagcg agggagcact
       61 tcaccgtgct ctcggttgac ctggctgtgg acagcgacaa taatggcatc attgagccag
      121 cggatgacgc agttgagacc ctcaatcccg ggaacctgat ttgcgtgaac cgagatgatg
      181 acaatatcaa cgatctgcag gacagggatg aggacgaaga ctttgtctat ggcgaagacg
      241 aattgtcgcc tgtcatcctg acatggcagg ggcctcgtgt cggggtgatc aggctttctc
      301 ttctggacac ggagatgagc gaaaggagcg ccgtgtacac caattctttc aagggagaca
      361 tcaaccgttt ggatctccct gtggagtgga atttgagaaa ctgtacacct cccggagttc
      421 tctacatcga gggagttgac cgggtgcgca atgatctcca tgtcgcatgg aaggttgata
      481 accagacaat ctgcgaagat gtcattcgct ttacggtgtt cgatatgaac atagcaatgg
      541 acggcaaccg agacggaacg atagacttca acaacccgga ggacgacaag tacgttttct
      601 gggtcaatga tgaccgggat gtgagatacc acgacgaaag tcctgactat atgtgttggg
      661 tcgaggatga cctacaggga ggaacatcag actgcgatga cgacagaatt ggcacggaca
      721 caagtccttt cgtccgtggc tgccgacgag atctggagga tttcactcgt gtgcatctgt
      781 ttgttgatga tgttgcggtc agctgtgaaa acctgaattt ctacctgaga ttcaagagag
      841 gaacccccag ccccacggtg aatctattca gcgcagtgga tggtggtagc acaagatact
      901 tgaccaatcc tgagaaagct gatgaacaga aggaggagac acgtctcttg acagttacga
      961 gcagcgaaat acagttgccg tcgggttatc cgaagacagg cggagcacct ttccatttca
     1021 tcctggaagg caaatcacct ggaaaaggcc agttctgcct gatcgccaaa gcgggcgaag
     1081 accatcgggc ggaggcgatt gtggatct
//
LOCUS       DAOQUC010001569         1108 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_193545_length_1108_cov_2.702754, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001569 DAOQUC010000000
VERSION     DAOQUC010001569.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1108
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_193545_length_1108_cov_2.702754"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..870
                     /locus_tag="PKH07_19990"
     CDS             <1..870
                     /locus_tag="PKH07_19990"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNT37284.1"
                     /translation="GVPFALWTKELDFLDVDPYNCLRDWLMLLPRHFKSWVLSSSRRL
                     SDRIKSESDAYLAFSEMAKEHIVSKGGKPGRIVQICNSIDMERFLARISESRQGDKLQ
                     DFRKQRGWEGKTVLLSLSYLLRRKGIHILLEAFSRLQVQDTVLAIVGDGPFRATLQET
                     ARRLGLQNVEFHPYTHDSHLYYLSSDALILPTLGPEPWGLTVNEALLCGIPAIVTTNV
                     GAREIIPHHELVVTPGDSEALCRAMQFIISDVALRESYRKLGPKIVHEECSVEQAGRG
                     LAEAFRLAVESSC"
     gene            complement(894..>1108)
                     /locus_tag="PKH07_19995"
     CDS             complement(894..>1108)
                     /locus_tag="PKH07_19995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37285.1"
                     /translation="AASVCWLAMPLFGTPNSVPGKIAMLATLCGLGGAVYVCFLLILK
                     EQTTLSLLGEIRKRLSSRKQERQTRA"
BASE COUNT          256 a          278 c          305 g          269 t
ORIGIN      
        1 ggtgttcctt ttgcactgtg gactaaggaa ttggatttcc tggatgtaga cccttacaac
       61 tgtctccgag actggctgat gcttctccca aggcatttca aatcatgggt gttgtcttcg
      121 agtcggcgtc tctcagaccg aatcaaaagc gaatcagatg cctatttggc ctttagtgag
      181 atggcaaagg agcatatcgt ttccaaaggt ggcaagcccg gccgcattgt tcagatatgc
      241 aacagcattg acatggaacg gtttctggcg cgaatctcag agtcccgcca gggagacaaa
      301 ctgcaggatt tccgaaagca gcggggctgg gagggcaaga ctgtcttgct ttctctctca
      361 tatctcctga ggcgaaaggg aatccatata ctgctggagg ctttctcacg acttcaggtt
      421 caggacactg ttctggcgat tgtcggtgac ggccctttca gagcaacgct tcaagagacg
      481 gcaagaagac ttggtcttca gaacgttgag ttccacccgt atacgcacga ttcccatctg
      541 tactatctct cttcagatgc attgatactg cccactctcg gcccagagcc ttggggcttg
      601 acggtgaatg aggcgctgct gtgcgggatt cccgccattg tgacaacgaa tgttggagcg
      661 agagagatca tccctcatca tgagctggtt gtcacaccgg gagactctga ggcgctctgt
      721 agagcaatgc agttcatcat ctcagacgtg gctctcagag aatcctatcg gaaactaggc
      781 cccaaaatag tgcatgaaga atgctcagtg gagcaagcgg ggcgcgggtt ggcggaggca
      841 tttcgacttg cagtcgaatc ctcgtgttga tttcgctggc attggagtca aagttacgct
      901 ctggtttgac gttcctgttt tcttgaagac agcctcttcc ggatctctcc cagtagagaa
      961 agagttgtct gctccttgag tatcaacaag aagcagacgt agacagcgcc accaagtcca
     1021 cagagcgttg cgagcatggc aatctttccc ggcacggagt ttggggtgcc gaacagcggc
     1081 atcgccagcc aacagacgct agcagcga
//
LOCUS       DAOQUC010001570         1108 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_193547_length_1108_cov_2.689459, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001570 DAOQUC010000000
VERSION     DAOQUC010001570.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1108
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_193547_length_1108_cov_2.689459"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            412..>1108
                     /locus_tag="PKH07_20000"
     CDS             412..>1108
                     /locus_tag="PKH07_20000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37286.1"
                     /translation="MDQLREPVTIATLIERFRKEYGRYSCKKIYQTVVHLLKRTFLDE
                     KSQRLLSQNPDFAPFIPKPKMRRLCPWSPPLVLQFLLNPIAKFLGTVPGLVFMGLSLG
                     ITFLMLMLFPGELEYLEVKGSYFMGALCWLCSLMIIAWLKNLFRFCLLKSRGLETPNS
                     GVLFQWGIPIFDVHDGDILTLGWAGVLRFHLACFLFTGLIGSLGIFGMLHGNVIATIW
                     VLSGLVTVFLDLSP"
BASE COUNT          237 a          271 c          283 g          317 t
ORIGIN      
        1 agggatagaa aaaggctgct tctccagtcc cgctgacatt aaggctttct ctagattata
       61 tcctgctcaa gacgtgtcaa gtgcaatgtc gaaatggcga aaatcagttg atctacgagc
      121 aggcagcatg tacgattcag atgggagtgg ctttgcgctg atgttatcgt gtggctggga
      181 gccataggtg atgagtttcg ttctgtgaac atgtgatttg atggactgat gatacggagc
      241 ccagattccg aggttctggg cgaggcgtca atgagacaac acgacagctt agaacagaat
      301 accgaaattc ggatcaagcc gtatgaggtt taccctttct cacgggactt tcttgcgaag
      361 aatcctgccg agaagaccta tctcttcgtt gactccgagg agctcttcct cttggatcag
      421 ctccgtgagc cggtgaccat tgccacgctg atcgagcgct tcaggaagga gtatggcagg
      481 tattcctgca agaagattta ccagacggta gtccatcttc tgaagcgcac atttctcgat
      541 gagaaatctc agcggttgct gtcgcagaat cccgatttcg ctccattcat acccaaaccg
      601 aaaatgaggc gactttgccc ctggtcacca cctctcgtgc tgcaatttct cctcaatccg
      661 atcgcaaaat tcctcggaac agttccggga cttgttttca tgggactatc tctgggcata
      721 acgtttctaa tgttgatgct ctttcctggc gaactggaat acctggaagt gaagggctcc
      781 tattttatgg gagccttatg ttggctctgc tctctcatga tcatcgcctg gctcaagaat
      841 ctttttcgct tttgccttct gaaatcccgg ggccttgaga caccgaactc gggcgtgttg
      901 ttccaatggg gcattccaat cttcgatgtg catgatggag atattctcac tctgggctgg
      961 gccggggtgc tccgatttca tctggcgtgc ttcctgttca caggacttat tggctctctg
     1021 ggcattttcg gcatgttgca tgggaatgtc attgccacaa tctgggtatt gagcggcctt
     1081 gtgactgtct ttctcgatct ctctccct
//
LOCUS       DAOQUC010001571         1106 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_194165_length_1106_cov_2.971456, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001571 DAOQUC010000000
VERSION     DAOQUC010001571.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_194165_length_1106_cov_2.971456"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(20..823)
                     /locus_tag="PKH07_20005"
     CDS             complement(20..823)
                     /locus_tag="PKH07_20005"
                     /inference="COORDINATES: protein motif:HMM:NF015013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HNT37287.1"
                     /translation="MNTPLNTQMSRRRLAAQTLLLTGGTFALKILAYVKLAILASFFG
                     TTSQNDAYVFALLLPSLIANVMTNSAAPALLPLFADLLFQGKREDAWRMAWTMVVSVT
                     LILTLAFLGVAVFYGRVVAPLASGFSPETRQICTQLFYIAGPSMILASVSACLRSLFQ
                     CLERFAVMTITQIVGLIVGIISLIALKPAYGIHAAALAVTLNYLAEVLFMIPFLWKEG
                     AFRFFHIGVSSRVVEASKLFLMYMVSSAVMQINTTIDRWLASSLAESSI"
     gene            complement(820..>1106)
                     /locus_tag="PKH07_20010"
     CDS             complement(820..>1106)
                     /locus_tag="PKH07_20010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37288.1"
                     /translation="GFLGVAGFLWTCLGSIWPLYRKPDQFGELGALGRSLFVGLVAML
                     GTGLTYQAFQWGTLITPLLVLSGCGLVLRRISQEEPFTKPSPEEAIQEVQ"
BASE COUNT          301 a          270 c          315 g          220 t
ORIGIN      
        1 tgctggcgtg attgatatac tagatgctgc tttccgcaag cgatgaagca agccatcgat
       61 ctatggtggt gttgatctgc atgactgctg aactgaccat gtacatcaga aaaagtttgg
      121 atgcctccac aactctgctc gaaacgccaa tgtggaagaa ccgaaaggct ccttctttcc
      181 aaagaaaggg gatcatgaag agaacttctg caaggtaatt gagggtcacg gcaagtgcgg
      241 cggcatgaat tccataggca ggcttcaggg caatcagaga gatgattccc actatgagtc
      301 cgacgatctg agtgatcgtc atgaccgcga agcgctcaag gcactgaaag agcgagcgca
      361 gacaagcgga aactgaagcc aagatcatgc tgggccctgc gatatagaac aattgggtgc
      421 atatctgtct ggtttccgga gaaaagccgg aagcaagtgg ggctacgact cttccataaa
      481 acacggccac gcccaaaaag gcgagcgtca gaatcagagt taccgagacg accattgtcc
      541 acgccattcg ccaggcatcc tctctcttgc cttggaagag cagatccgcg aacaagggca
      601 ggagtgcggg agccgctgag ttggtcatga cattggcgat cagggaaggg agcaagaggg
      661 caaacacata ggcatcattc tgggacgttg ttccaaagaa ggaggccaag atcgccagtt
      721 tcacgtatgc aagaatcttc agggcaaagg ttccacccgt cagcagtaag gtctgagcag
      781 ccagcctgcg cctggacatt tgggtgttga gaggagtatt cattggactt cctgaatggc
      841 ctcttcagga gaaggttttg tgaaaggttc ttcctggctg atgcgtctga gtacgagacc
      901 gcatcccgag agtaccagaa gtggggtgat gagcgttccc cattggaatg cctgataggt
      961 gagccccgtt cccagcatgg ccacaagacc gacaaacagg gaacgtccaa gtgcaccgag
     1021 ttcaccaaat tgatcgggct ttcggtaaag aggccagatt gagcccagac acgtccagag
     1081 aaacccagct acgcccagga atcccc
//
LOCUS       DAOQUC010001572         1105 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_194415_length_1105_cov_3.998095, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001572 DAOQUC010000000
VERSION     DAOQUC010001572.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1105
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_194415_length_1105_cov_3.998095"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            67..753
                     /locus_tag="PKH07_20015"
     CDS             67..753
                     /locus_tag="PKH07_20015"
                     /inference="COORDINATES: protein motif:HMM:NF036525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNT37289.1"
                     /translation="MAIGSTDLESERSDRRTRRRQKMCEDILEAAEKVIAQRGLEKAS
                     MEEIADQAAIAKGTIYLYFKNKNDLLLDLVRRNHEEIEAEVALILEQERTPGEQIRAV
                     VRQMLKYRREKFEVHRVLFTQYLRADDAQDPVIREMKQAMEKRVQMVMDIYREGVRQK
                     EFRDLDPLFAVEMLSGMFHQFLLRDLSKPGGCPPEEQGDQILDLFFHGVLMPSEKTKS
                     NSTARSHAKR"
     gene            750..>1105
                     /locus_tag="PKH07_20020"
     CDS             750..>1105
                     /locus_tag="PKH07_20020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37290.1"
                     /translation="MKKYSLLVSVALLFGQTPDVFAQHVPAPAGIPSVSRSLGLRECV
                     LQSLENNLQVRVQRYVPLLDQADIEIEKAFFDPVFRAGASQTGYQHRERIIWAPSGAQ
                     LNRDRAEVFYSEISKQL"
BASE COUNT          275 a          273 c          313 g          244 t
ORIGIN      
        1 gagttagata tatgaccaga cggtcatatt gtgaccgagt ggtcatagcg aggaggaatt
       61 gacaccatgg cgatcggttc aacggatctg gagtccgagc gatctgaccg acgcactcgg
      121 cgaaggcaaa agatgtgcga agacatcctg gaagccgccg agaaagttat tgcgcagcga
      181 ggtcttgaga aagccagcat ggaagagatt gccgatcagg cagccatcgc caaggggacg
      241 atctacctct attttaagaa caagaacgac cttcttctcg atcttgtccg ccgaaatcat
      301 gaagagattg aagcggaagt cgcactcatc cttgagcagg aacggactcc cggtgaacag
      361 attcgagcag tggttcgaca gatgctcaag tatcgtcgtg agaagtttga agtgcatcgc
      421 gtgcttttca cgcagtacct gcgagcagac gatgcccagg atcccgtcat tcgagagatg
      481 aaacaggcca tggagaagcg tgtccaaatg gtcatggata tctatcggga aggggttcgg
      541 cagaaagagt ttcgcgatct ggatccactt tttgcagtcg aaatgttgtc cggcatgttc
      601 catcagtttc tcctgagaga tctcagcaaa ccgggtggct gtccaccgga ggaacagggc
      661 gatcaaatac tcgatctttt cttccatgga gttctgatgc cctcggagaa aacgaagtcc
      721 aattccacgg caaggagtca cgccaaacga tgaagaagta ctcgttgctt gtctctgttg
      781 ctcttctgtt cggacaaact ccggatgtgt tcgcgcaaca cgttccggct ccagcgggga
      841 ttccgtccgt atcccgctct ctgggtcttc gcgagtgtgt gctgcaaagt ttggagaaca
      901 atcttcaggt ccgagtgcag cgctacgttc cccttctgga tcaagcagac atagagatag
      961 aaaaggcctt ctttgatccg gtgtttcgtg ccggagcgag ccagacagga tatcaacatc
     1021 gcgagcgcat catctgggcg ccgagcggcg cacagttgaa ccgcgaccgc gcggaggtct
     1081 tctactcgga gataagcaag caact
//
LOCUS       DAOQUC010001573         1105 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_194428_length_1105_cov_3.551429, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001573 DAOQUC010000000
VERSION     DAOQUC010001573.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1105
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_194428_length_1105_cov_3.551429"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="PKH07_20025"
     CDS             <1..229
                     /locus_tag="PKH07_20025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013643702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="branched chain amino acid aminotransferase"
                     /protein_id="HNT37291.1"
                     /translation="GLRIEQHALPRESLYIADEVFLTGTAAEITPVTTIDNITIADGE
                     RGPITKAVQDEFFAILKGTKEDKHNWLSHVR"
     gene            complement(288..875)
                     /locus_tag="PKH07_20030"
     CDS             complement(288..875)
                     /locus_tag="PKH07_20030"
                     /inference="COORDINATES: protein motif:HMM:NF018701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1318 domain-containing protein"
                     /protein_id="HNT37292.1"
                     /translation="MERAKMSVFWCGVVLAFMVSCFPITINVYFPETKIESAAAQIES
                     EIRGDTGQQGQGTSWRLPRMRLAWGIPQAFAQEPDLKVDTPAIQEIKNTRKARFPQID
                     ELLTKAFVGEGKDGLLKEKDSGVLQLKELAQARKLVKEENADRLRLYKEIATENGFPE
                     EVSRIQEIFGRENRKQIKIGQYYEDDDGTWKQKKE"
     gene            complement(889..>1105)
                     /locus_tag="PKH07_20035"
     CDS             complement(889..>1105)
                     /locus_tag="PKH07_20035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37293.1"
                     /translation="RARLEKNIISMVGTVPQRGQDYVMLGAGWWTPLNIVFHSSERVA
                     FKEFVERMKMHFENVQNESEGISIRTN"
BASE COUNT          251 a          322 c          242 g          290 t
ORIGIN      
        1 gggactgcgc atcgaacagc acgctcttcc ccgtgaatct ctctatattg ctgatgaagt
       61 cttcctgact ggcactgccg ctgagattac cccggtcacg accatagaca acattacgat
      121 cgccgatggt gagcgcggac cgattaccaa ggcggttcaa gatgagtttt tcgcgattct
      181 gaagggaaca aaagaagaca aacacaactg gctgagtcac gtccgatagc aatgccaagg
      241 gtctcccgga gacgagaaca agttcgggag accctgtggc aatttctcta ctctttcttc
      301 tgcttccacg taccgtcgtc gtcttcgtag tactgaccaa tcttgatctg ctttcggttt
      361 tcacgaccaa agatctcctg aatacgggag acttcttcgg ggaaaccatt ctcggtagcg
      421 atctctttgt agagcctcag gcgatcggcg ttctcttctt tgacgagttt cctcgcctgg
      481 gcaagttctt tcagttggag aacaccactg tccttttctt tgagaagtcc gtctttgcct
      541 tctccgacaa aggcctttgt cagcagttcg tcaatctgag gaaaacgtgc cttgcgagta
      601 ttcttgatct cctgaattgc cggagtatcc accttcagat ccggttcctg cgcgaaagct
      661 tgcggaattc cccaagccaa tctcattctg ggaagtcgcc aggaagtgcc ctgcccctgt
      721 tgccctgtgt caccccgtat ctcagactca atctgcgctg cagcactttc aatcttcgtc
      781 tccggaaagt acacattgat cgtgatcgga aaacagctga ccatgaaggc caagactacg
      841 ccacaccaga aaacactcat ttttgctcgt tccatagcag attcctcctc agttcgtccg
      901 aatggaaata ccttcgcttt cgttctgcac gttctcaaaa tgcatcttca tcctctccac
      961 aaactccttg aatgccactc gttcgctgga gtggaagacg atattcaacg gcgtccacca
     1021 tcccgctccc aacatcacat agtcctgacc acgctgtgga actgtaccga ccatgctgat
     1081 gatattcttt tccagacgcg cacgc
//
LOCUS       DAOQUC010001574         1105 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_194475_length_1105_cov_2.728571, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001574 DAOQUC010000000
VERSION     DAOQUC010001574.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1105
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_194475_length_1105_cov_2.728571"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /locus_tag="PKH07_20040"
     CDS             complement(<1..358)
                     /locus_tag="PKH07_20040"
                     /inference="COORDINATES: protein motif:HMM:NF021882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37294.1"
                     /translation="MALGSAERRGELNQGLSNKETLDLLSSSDTIAAPATPRGKSAIG
                     IVRLSGSQSPVIADRIFRPSQGTLKPSERQERKLIHGSIVDPQTGEFVDEALLVLMKA
                     PHSYTGEDMCEIQAHGS"
     gene            complement(429..1007)
                     /locus_tag="PKH07_20045"
     CDS             complement(429..1007)
                     /locus_tag="PKH07_20045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37295.1"
                     /translation="MEQPNGTSPATLTLQTREVRLLARVRDLLEAMGPMSADRIAVRL
                     KEKHILVLSLLDKAKAAGELYCDKQGRFALNLEQCVEETEQQTVSSETEPEANAESRL
                     LQARTFLKLWRILKADSRLTVTVGKSVDACRSGVLVARVRVEESAIPMVELWEPSRSS
                     DSGQQRTQVFRIWNKPQATVAIKALKERLGNL"
BASE COUNT          227 a          312 c          289 g          277 t
ORIGIN      
        1 cgcttccatg cgcttgtatt tcgcacatgt cctcgccggt gtaggagtga ggggctttca
       61 tgagaaccag caaggcttcg tcaacaaact ctcccgtctg aggatcgaca atgctcccgt
      121 gaatgagttt tcgctcttgt ctctcggatg gtttgagcgt gccttggctg ggtcggaaaa
      181 tgcgatcggc aatgacaggg gactgtgaac cactgagtcg aacaatcccg atggcgctct
      241 tgcctctcgg tgtggcaggc gcggcaatgg tgtcactgct tgaaagaaga tcaagtgttt
      301 ccttgttcga aagtccctgg ttcaattctc cgcgcctctc tgcgcttccc aaagccacaa
      361 ctctgtctcc ccatgaacga aagggaaacc ttcacacacc acatccgatc cacgaatgac
      421 gcgaatcgtc agagattgcc aagtcgttcc ttgagggcct tgattgccac agtcgcttgc
      481 ggtttgttcc agattcgaaa aacctgagtc ctctgttgtc cactatcgct gctgcgggaa
      541 ggctcccaga gttcaaccat gggaatagcg gattcttcga ctcgaacacg agccacgaga
      601 acgccactgc ggcacgcatc cacgctcttg ccaacggtca cagtcaaacg ggagtccgcc
      661 ttgagaatgc gccacagttt gaggaatgtt cgtgcttgga ggagacgact ctccgcattg
      721 gcctcaggct ctgtctcact ggaaaccgtc tgttgttctg tttcttcgac acattgctca
      781 agattcaggg caaagcgccc ctgcttgtca cagtagagtt caccagcagc ctttgccttg
      841 tcgagcagtg acaggacaag tatgtgtttt tctttcaacc tcacggcgat cctgtccgca
      901 gacatgggac ccatcgcttc gagcagatcg cgaacacggg caagcaggcg tacttcccgc
      961 gtctgtagtg tcagtgtggc tggactcgtg ccgtttggtt gttccatctg tgatgttttt
     1021 cggctcgtgc tcaaggggcg atcaggatca tatcagcaat tccctccagc acgattccct
     1081 ccttctcgat cttgaggacg cgatg
//
LOCUS       DAOQUC010001575         1104 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_194740_length_1104_cov_3.948522, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001575 DAOQUC010000000
VERSION     DAOQUC010001575.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1104
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_194740_length_1104_cov_3.948522"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..912
                     /gene="cysK"
                     /locus_tag="PKH07_20050"
     CDS             <1..912
                     /gene="cysK"
                     /locus_tag="PKH07_20050"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010907957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HNT37296.1"
                     /translation="ITELVGNTPLVFLNRVTEGSHARVAAKLESFNPISCVKERIGLA
                     MIEAAEKAGLIKPDTIILEPTSGNTGIGLAFVCAARGYKLVLTMPESMSEERRRILRA
                     FGAEIVLTPAAKGMTGAVEQAKEMAAQDSRYFIPQQFENPANPEIHRKTTAEEIWRDT
                     DGKVDIFVAGVGTGGTITGVGELLKERKPAVRIIAVEPVESPVLSGGKPGAHGIQGIG
                     AGFVPKVLNRAILDEVIQVETNQARAMGRRLAQEEGIFAGVSSGAAVEAAVTVARRPE
                     NAGKLIVVILPDTGERYLSTPLFAETS"
     gene            965..>1104
                     /locus_tag="PKH07_20055"
     CDS             965..>1104
                     /locus_tag="PKH07_20055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37297.1"
                     /translation="MIRESDNAVAVLMSGGVDSSVAAFLLKQAGCGPVGVTLFLGSTS
                     GM"
BASE COUNT          246 a          280 c          344 g          234 t
ORIGIN      
        1 atcacagaac ttgttggcaa cacccctcta gtttttctga accgtgtcac tgaaggaagc
       61 catgcgcgtg ttgccgccaa actcgaatcc ttcaacccaa tctcctgcgt caaagaacgc
      121 attggtctgg cgatgattga ggcggcagag aaagcgggct tgatcaaacc ggacactatc
      181 attctggaac cgacttcggg aaacacaggc attggactcg ctttcgtctg cgccgcgcga
      241 ggatataaac tggtgttgac gatgcccgag agtatgtctg aggaacgccg aagaatcctt
      301 cgagcgtttg gcgcagagat cgtcttgacc cctgcggcga agggcatgac aggcgccgtt
      361 gaacaagcca aggaaatggc ggctcaggat agtcgttact tcattcccca gcaattcgag
      421 aatccggcaa atccggagat ccaccgcaag accacagctg aggagatctg gcgggacacg
      481 gacggaaagg tggacatctt cgtggcgggc gtcggaaccg gtggaacgat caccggggtt
      541 ggagaactac tcaaagagcg gaagcccgca gtgaggatca ttgccgttga gccagtcgag
      601 tcgcccgttc tcagcggtgg caagccaggg gcgcacggca tccaaggcat cggtgcggga
      661 tttgttccca aggttctcaa ccgcgccatt ctcgatgagg tgattcaggt tgaaaccaat
      721 caggcgagag ccatgggacg acgtcttgct caagaggaag ggatcttcgc aggagtctct
      781 tccggtgcgg ctgtcgaagc ggcggtgaca gtggcacgca gaccggagaa cgcaggcaag
      841 ttgatcgttg tcattcttcc ggacacggga gagcggtatc tttcaacacc gttgtttgct
      901 gagacttcct gattccctgg ctccttcgga gtgactgtgt cctttgaacg tcaaggatgg
      961 cacgatgatc cgagagagtg acaatgccgt cgccgttctg atgagtggcg gtgttgacag
     1021 tagtgtggct gcgtttctgc tgaagcaggc agggtgcggg ccggttggtg tgacattgtt
     1081 ccttggttcc acatccggca tgga
//
LOCUS       DAOQUC010001576         1102 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_195338_length_1102_cov_3.960840, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001576 DAOQUC010000000
VERSION     DAOQUC010001576.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1102
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_195338_length_1102_cov_3.960840"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1102)
                     /gene="glgP"
                     /locus_tag="PKH07_20060"
     CDS             complement(<1..>1102)
                     /gene="glgP"
                     /locus_tag="PKH07_20060"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HNT37298.1"
                     /translation="QYLNNDGWQMETYPENDWYNMPVRIERSQDGQPVEISVQMDGYD
                     IHAQVWLAEVGLTRLYLLDTNIPKNPPHLRNITHRLYGGDRERRMQQELLLGIGGVRA
                     LKTLGIQPTVYHMNEGHSAFLVLERFREAMKEKTLSFEEARELIWASSVFTTHTPVPA
                     GNERFSLDLMTKYVKPLVDDLGLDWHSFLALGKVNPHDKEETFCMTVLALKLSSLNNG
                     VAKIHGRVSREMWKGMWPDLPVSEVPIRSITNGIHTPTWISHDLRELLASYLGPSLQE
                     RPWDLKVWDAAERIPDIEFWRTHQRRRERLVFFARKRLRQQFERQGLRSEAVERAEGI
                     MNPHALTLGFARRFATYKRGNLLFTDPDRLYRL"
BASE COUNT          259 a          304 c          269 g          270 t
ORIGIN      
        1 caaacggtaa agtcgatcgg gatcagtaaa aaggagattg ccacgcttgt aggtggcaaa
       61 acgccttgca aagccgagag tcaatgcatg ggggttcatg attccctcgg ctctttcaac
      121 tgcctcggaa cgcaatccct gccgttcgaa ctgctggcga agacgcttgc gagcaaagaa
      181 gaccaaacgt tcccttcttc gctgatgcgt tcgccaaaac tcaatgtccg gaatccgttc
      241 tgccgcgtcc caaaccttca aatcccatgg tctctcctga agactcggtc caagatacga
      301 agccagaagt tcccggaggt catggctgat ccaagtcgga gtatggatac cattcgtgat
      361 gcttcgaatg ggcacctcgc tgacgggcag gtcaggccac atccccttcc acatttctct
      421 tgagacccga ccatgaatct tcgccactcc attgttcagt gatgagagtt tcagggcgag
      481 aacagtcatg cagaatgttt cttccttgtc atggggattg actttgccca aagcgagaaa
      541 ggaatgccag tcaagtccca gatcgtctac cagtggtttc acatacttgg tcataagatc
      601 caaggagaat cgttcgttcc cagcaggaac cggcgtgtgt gttgtgaaga cagaacttgc
      661 ccagatcaat tctcgggctt cttcaaaact cagagtcttt tccttcatcg cctctcggaa
      721 tctctccagc acgaggaacg ccgagtggcc ttcgttcata tgatagacgg tgggctgaat
      781 tcccagagtc ttcaacgcac gtaccccgcc gatgccgagc aacaattcct gctgcatccg
      841 cctttctctg tcccctccgt agagccgatg ggtgatattg cgaagatgcg gtggattctt
      901 gggaatgttt gtgtcgagca gatacagtcg agtcaatcca acttcggcga gccagacttg
      961 agcatggatg tcgtatccat ccatctgaac agaaatctca accggttgcc cgtcctggct
     1021 tcgctcaatc cgaaccggca tattgtacca gtcattttca ggataggtct ccatctgcca
     1081 cccatcgttg ttcaggtact ga
//
LOCUS       DAOQUC010001577         1102 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_195395_length_1102_cov_2.698185, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001577 DAOQUC010000000
VERSION     DAOQUC010001577.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1102
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_195395_length_1102_cov_2.698185"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..241)
                     /locus_tag="PKH07_20065"
     CDS             complement(<1..241)
                     /locus_tag="PKH07_20065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37299.1"
                     /translation="MAPPREMRYGCLLFLCLFCAFPQIAGCVEFKVDPSLTLESWMTY
                     DDNIYLTTRRDLEAEIDDPLRYRPGTSDVFSRLVGD"
     gene            complement(322..>1102)
                     /locus_tag="PKH07_20070"
     CDS             complement(322..>1102)
                     /locus_tag="PKH07_20070"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HNT37300.1"
                     /translation="LMILATVGISTSPAQESGNANVVSVSGEASMQMPPATEWQAVEA
                     GASLPQGTQIRTEPAASVLIEFKPGHRSQIAGGSFVTLSELSYAEEQGPEKTTVDLAV
                     GKILSNVKKLRTTNSEYSVRTPTASAAVRGTVYEVVYDPGTRHTQVKVLEGLIHLESR
                     IGTRLSGEINEREKSGVDASGALSPSEPMSEQETNEMQQASQQAVTSQAGTSEVQGQT
                     GQLDATQSEVTGRLEEIQERLSEENRANRIDSLREIDDDDD"
BASE COUNT          247 a          301 c          285 g          269 t
ORIGIN      
        1 ggtcaccgac gagtctcgaa aaaacgtctg acgttcccgg gcgatagcga agaggatcgt
       61 caatttctgc ttcgagatct cttcgggtgg taaggtagat gttgtcatcg taggtcatcc
      121 acgactccaa ggtgagcgat ggatcaacct taaactcaac gcaccccgcg atctgcggga
      181 acgcacagaa cagacacaaa aacaacaaac acccatacct catctcgcga ggtggtgcca
      241 tatcaaaacc ccttcaaagt tgtttctagg cctgcagaaa gccgtttatc aaactgcaca
      301 tgtcacgaag tcatgacatg gctaatcatc atcatcgtca atttctcgaa gagagtcaat
      361 ccggttcgct cgattttcct cagaaagtct ttcctggatc tcttccagac gaccggttac
      421 ctcggactga gttgcatcca actgtccggt ctgaccttga acttccgaag ttccagcctg
      481 agaagtcacg gcctgttgtg aggcctgttg catctcattg gtttcctgct cggacatcgg
      541 ttcagatggg gagagcgctc cagaggcgtc cacgccagac ttctcccgtt cgttgatctc
      601 gcccgaaagg cgtgtgccga tgcgagactc cagatggata agtccttcta gaaccttcac
      661 ttgtgtgtgt cgagtgcccg ggtcgtagac aacttcgtag actgttccac gaaccgcagc
      721 agaagccgtt ggcgttctga cagagtactc cgaattggtg gtgcggagtt tcttgacgtt
      781 gctgagaatc ttgccaactg ccaggtccac cgtggtcttc tcgggacctt gttcttcggc
      841 ataggacaat tctgaaagcg taacaaaact cccacctgcg atctgactgc gatggccggg
      901 tttgaactcg ataaggacgc ttgcagcagg ttcggtgcga atctgagtgc cttggggcag
      961 cgatgctcca gcctcgactg cttgccactc cgtcgccggt ggcatttgca ttgaagcttc
     1021 ccccgatacg ctgaccacat tggcattgcc ggattcctga gcaggtgatg tgcttatgcc
     1081 gaccgtggct aagatcataa gg
//
LOCUS       DAOQUC010001578         1102 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_195408_length_1102_cov_2.594078, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001578 DAOQUC010000000
VERSION     DAOQUC010001578.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1102
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_195408_length_1102_cov_2.594078"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1102)
                     /locus_tag="PKH07_20075"
     CDS             complement(<1..>1102)
                     /locus_tag="PKH07_20075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012530332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HNT37301.1"
                     /translation="TIMVNSNPETVSTDYDTSDRLYFEPLTFEDVLNIYEAEKPLGVI
                     LQFGGQTPLNLALSLQEAGVNVLGTDPKNIHKAEDRKHFAKMLDDLKIPYPPAGTARS
                     LDETREIVSRIGYPVLLRPSYVLGGRAMVIVHQEEELERCFNLALEASPEHPVLIDKF
                     LDESIEVDVDAICDGESVYIGGIMEHIEYAGVHSGDSACVLPPRTLSEETIQTIRDYT
                     TKIAFAFEVRGLMNIQYAVSPSSSPSGKPTVYVLEVNPRASRTVPFVSKATGVPLAKV
                     AARVMLGQTLKKQGVVGIRHMPYLAVKEAVLPFVKFPGVDIILGPEMKSTGEVMGIGK
                     QFGTAFAKAEAGAWCSLPDPRIASAQGKKVFLS"
BASE COUNT          254 a          299 c          292 g          257 t
ORIGIN      
        1 cacttaagaa gaccttcttg ccttgcgcag aagcgattcg aggatcagga agcgaacacc
       61 aggctcccgc ttccgccttg gcgaatgccg tcccaaattg ctttccgatg cccatgactt
      121 cgcccgtgga tttcatctcg ggtccgagga tgatatccac gcctggaaat ttaacaaagg
      181 gcaacacagc ttccttgact gccagatagg gcatgtgtcg gattccgaca actccctgct
      241 ttttcaatgt ctgtcccaac atgacccggg cggcaacttt cgcaagaggc actcctgttg
      301 ctttgctgac aaagggaacc gttcgagacg cccgagggtt gacttccagc acatagacgg
      361 ttggtttgcc tgaagggctg gaagaaggag agacggcgta ctggatattc atcaaacctc
      421 ggacttcaaa ggcaaaggcg atcttggtgg tgtaatcccg aatcgtctgg attgtctcct
      481 cactgagcgt tctgggtggc aacacacagg cactgtcacc cgaatgaacg ccggcgtatt
      541 cgatatgctc catgattcca ccgatgtaga cgctctcgcc gtcgcagatg gcatccacat
      601 ctacctcgat agattcatcc aagaacttgt cgatcaagac agggtgttcc ggtgaggctt
      661 cgagcgccag attgaaacag cgctccagtt cctcttcctg atgcacgatg accatggcac
      721 gaccaccaag aacgtaactg ggacgcaaca aaacgggata tccgattcgg ctcacgatct
      781 cccgggtctc atcgagactt cgcgctgttc cagcaggcgg gtaagggatc ttcaaatcgt
      841 caagcatttt ggcgaaatgt ttacggtctt ccgccttgtg aatgtttttg gggtcggttc
      901 ccagaacatt gacacctgct tcctgcaggg acagagccag gttcagaggg gtctgtccgc
      961 caaactgaag aatgacaccc aacggcttct cggcttcgta gatgttcagc acatcctcaa
     1021 aggtcagcgg ctcgaagtag agccgatccg aggtgtcata gtcggtgctg acggtttcag
     1081 gattgctgtt gaccatgatg gt
//
LOCUS       DAOQUC010001579         1101 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_195619_length_1101_cov_4.462715, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001579 DAOQUC010000000
VERSION     DAOQUC010001579.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1101
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_195619_length_1101_cov_4.462715"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            100..>1101
                     /locus_tag="PKH07_20080"
     CDS             100..>1101
                     /locus_tag="PKH07_20080"
                     /inference="COORDINATES: protein motif:HMM:NF016991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HNT37302.1"
                     /translation="MMIWGFWKSYQARKRLEAAQYELADRLIREASSLVPSDESESDW
                     ALIGGRRSQWGLSRDLWTEEHRQRVQDRAYELYRTNEHARSVIRNLERYIIGPKVAFT
                     PLDDNPVVLQWWQEFESRNRFILKQKEIVRRTFRDGECFIRFFENSDTGRMTLRFIEA
                     QHIQDPMHRFSHGIHTEADDHPDGDVEEIIGYVVSADGTSCTEIPAGQIMHIKVFADS
                     NEKRGNSIFELLGTSIEAYQRWLRDRMVLNRIRSFFGIHRKISHATPGQVASLAAQSA
                     VNRTADRQGRNRQEPMKAGSIITTSDNVELVPIAANLQASDAQYDGRNILLTIAAGCG
                     "
BASE COUNT          269 a          290 c          312 g          230 t
ORIGIN      
        1 gcactggcac ggacgtcggc attcgattgt attgagcaac ccgatatggc gggagacatt
       61 ttcttgtgaa agcaaccatc acctatagag gaggttcgaa tgatgatatg gggattttgg
      121 aagagttatc aggctcgaaa acgattggag gctgcccagt acgaactggc agatcgcctc
      181 attcgagagg catccagtct ggttccaagc gacgaatcgg aatctgactg ggcgttgatc
      241 ggcggacgcc gctcccaatg ggggctgagt cgggatctct ggactgagga acaccggcag
      301 agagttcagg acagagcgta tgaactctac cgaaccaatg aacatgcccg ttccgtgatc
      361 cgaaatcttg agcgctacat cattggtccg aaggttgcct tcacgccgct ggacgacaac
      421 cctgtcgtgc tgcagtggtg gcaggagttt gagagtcgca accgttttat cctgaaacag
      481 aaagagattg tgcgccggac attccgtgat ggagagtgct tcatccggtt cttcgaaaac
      541 tcggataccg gtcgcatgac gctccgattc atcgaagccc aacacatcca ggatcccatg
      601 catcggttca gtcacggcat ccatactgag gcagacgacc atcccgatgg agatgtggaa
      661 gagatcatcg gctacgttgt ctcggcggat ggcacgtctt gcactgaaat tcccgcggga
      721 cagatcatgc acatcaaggt ctttgccgac agcaatgaga aacggggcaa ttcaatcttc
      781 gagctcctgg gaaccagcat tgaggcatac cagcgatggc tgcgtgatcg gatggtgctc
      841 aatcgaatac gctcattctt cggaattcac agaaaaatct cacacgcaac gcctggtcag
      901 gtcgcctcac ttgcagcgca gtccgcggtc aaccgaactg cagaccggca gggtagaaac
      961 cgacaggagc cgatgaaggc gggttccatt atcactacct cagacaacgt tgaactggtg
     1021 cccatcgctg ccaaccttca ggcgtccgat gcacagtacg atgggcgcaa catcttgttg
     1081 accatcgccg cggggtgtgg c
//
LOCUS       DAOQUC010001580         1101 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_195693_length_1101_cov_2.814532, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001580 DAOQUC010000000
VERSION     DAOQUC010001580.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1101
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_195693_length_1101_cov_2.814532"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(255..650)
                     /locus_tag="PKH07_20085"
     CDS             complement(255..650)
                     /locus_tag="PKH07_20085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37303.1"
                     /translation="MFRGEKNGQGLSGRVVIAIPYILAWFHRTACRGFGLVALRQGVR
                     IEADVRILRGATGHIRIWESEVVPQMMGSTTVFRKRDGLLTGTSEAVVRRECSALWPG
                     ADELDDVAVRVLGEDQVAVATEVVVGGEA"
     gene            complement(830..>1101)
                     /locus_tag="PKH07_20090"
     CDS             complement(830..>1101)
                     /locus_tag="PKH07_20090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37304.1"
                     /translation="KPVEAPSDFAATDNPISRLYQSARTDAERQYADRWDWLFVTKMW
                     LADDMGTTEASKRERRISLVVQTHPQVRYVTESKSRLDRSEFGEP"
BASE COUNT          266 a          351 c          261 g          223 t
ORIGIN      
        1 ggcggcgaca gccacctgat cctcgtcaag gacccggacg gcaacatcat cgagctcatc
       61 ggccccaggc tataacgcaa gacattctct ccgcaggacg cacgacgaag cagagcaaga
      121 aggtgagaag atcagaaggt cggaaggggc agacaacgaa acgcaagaac ggaccggcaa
      181 cggaaggtga gcgggcagga aaggcacctg ggaagcggcg acagcggtag ggtgggctcc
      241 gcccaccgtt ctgatcacgc ttctcctcct actacaacct cggtggcgac agccacctga
      301 tcctcgccaa ggactcggac ggcaacatca tcgagctcat cggccccagg ccataacgcg
      361 gagcactctc tccgcacgac cgcttcggac gtgcctgtca gaaggccgtc tcgtttccgg
      421 aaaacggtag ttgaccccat catttgcggg acaacctctg attcccaaat ccggatgtgc
      481 ccagtagcac ccctcagaat ccgaacatcc gcttcaatcc gaacaccttg tctaagagcc
      541 acaagcccaa acccacgaca agcagtccga tgaaaccaag ccaaaatgta aggaatagcg
      601 ataacaactc gtcccgaaag cccttgccca ttcttctccc ctcgaaacaa ggattcttct
      661 ctcttcgtga tttcactatc atgatacttc ccatatgacc tttgcccagc agtcattgtc
      721 acgcgaaatc agaaccagcg acggctcccg gacaatctat agttcccaat tcaggatgtg
      781 tcccctggtt tccctcaagc ctgatccttt ctctctcgtc cgtgacgcct cagggttcac
      841 cgaactcgct cctatcaagc ctagacttcg actccgtaac ataacggact tgcgggtgtg
      901 tctgaacaac cagagagatt ctccgttccc tcttggatgc ctctgtcgta cccatgtcat
      961 cagcgagcca catctttgtg acgaacagcc agtcccaccg gtctgcatat tgcctctctg
     1021 cgtcggtacg tgcactttga tagagcctgc taatcggatt gtctgtcgct gcaaagtccg
     1081 aaggcgcttc tacgggctta t
//
LOCUS       DAOQUC010001581         1100 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_195991_length_1100_cov_3.014354, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001581 DAOQUC010000000
VERSION     DAOQUC010001581.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1100
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_195991_length_1100_cov_3.014354"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1100
                     /locus_tag="PKH07_20095"
     CDS             <1..>1100
                     /locus_tag="PKH07_20095"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNT37305.1"
                     /translation="RDSDALQAVGEDARAEDEAVRLAALYALSEIGEVSSKEILAEAA
                     ETITGFHRVEATHFYLTFAQRLAEKGHTEEALAVCRDLFESKTAPDETAVQCAALHVM
                     SQALGENAIPDLLQAMTNDKEQVRAQAMNITNSFSGNRITRGLMEFYQTAFPDLQAEL
                     IRLLARRGDNKALPLLFAGLDSSEKVVRLAAIESVSTLAGSASLNALLDVFSNEDKEE
                     VEAAKAALTMMPGTQATADIAAGVTDPHPAIRLGSIEILVARGAKDQIQKLCLAGRDD
                     DEDIRLAALKGLGALGDETVAADLVALLLEASSVKERKEAEKALNLVLERSKDRSSAE
                     RPIVEGIESGVSSEIRSALLTALKYVGTPKAL"
BASE COUNT          255 a          285 c          316 g          244 t
ORIGIN      
        1 tgcgggattc agacgcgctt caagcggttg gcgaagatgc cagggcagaa gacgaagccg
       61 ttcgcctggc cgcactctat gctctgagtg aaatcggcga ggtttcctcc aaagagattc
      121 tcgctgaagc ggcggaaacc atcaccggat ttcatcgtgt ggaagccacc catttctatc
      181 tgacgttcgc gcaacggctt gcggaaaagg gacataccga ggaagccctc gcggtgtgtc
      241 gtgacctttt cgaatccaag actgcccctg atgaaaccgc agttcaatgc gcagcgctcc
      301 atgtcatgtc tcaggcgctt ggagagaatg cgattccaga tctcctccag gcgatgacca
      361 atgacaaaga acaggttcgt gcacaggcca tgaacatcac aaactctttc tcaggcaacc
      421 gaatcacgag aggcctgatg gagttctatc aaaccgcatt ccccgatctg caggcggagt
      481 tgatccgcct gttggctcgt cgcggtgaca acaaggcact gcctttgctt tttgctggac
      541 tggacagttc cgaaaaagtt gtccgcttgg cggccattga atcggtgagt acgttggcag
      601 gcagtgcatc cctgaatgct ttgttggatg tcttttccaa cgaagacaaa gaggaagtcg
      661 aggctgccaa ggctgcgctg acaatgatgc cgggcacaca agccacggca gatattgctg
      721 ctggtgtgac cgatccccat ccggcgattc gacttggctc catcgaaatc cttgttgctc
      781 gtggcgcaaa ggatcaaatt cagaaactct gccttgcagg ccgagatgac gatgaggaca
      841 ttcgactcgc ggcactcaaa gggcttggtg ctctgggtga tgagactgtc gcagcggatc
      901 tcgtggctct gttgcttgag gcctcgtctg ttaaggagcg aaaggaagcg gagaaggcgc
      961 tcaatctcgt gctggagcgt tcaaaggaca gaagttccgc cgaaagaccc atcgttgagg
     1021 gaatcgaatc gggtgtttcc agtgagattc gcagcgctct tttgacagca ctcaagtatg
     1081 ttggcactcc gaaagcattg
//
LOCUS       DAOQUC010001582         1100 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_195998_length_1100_cov_2.884211, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001582 DAOQUC010000000
VERSION     DAOQUC010001582.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1100
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_195998_length_1100_cov_2.884211"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1100
                     /locus_tag="PKH07_20100"
     CDS             <1..>1100
                     /locus_tag="PKH07_20100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37306.1"
                     /translation="ADATGKAPDTLLMQLDTQLQAQLSQTKKIPLVERADDRYLDELF
                     FGTKDGWVDPERIPKTGKLVGAKYYLSTLVTKYSASAWGDNAALPNVYENSWSVSVEV
                     TGKVINVETGQLLETILVTESSSGKSEDKEKMTSVPNLNGILGSLAQKATGEIVDQLL
                     VILFPALIADVSETEIFLNQGEATGVKVGDRYTVFSQGKAIVDPQTGRTLGNREQKIG
                     DIEIVEVRREYSTAKSADVALSEFAPGMVCRPVTGAKKSEPVVKDSQPSVSSSGATSL
                     GDTPKTSKPKEETAPKEPKGRVFCLAEMLNSTSAPRRVEQAIHGYLSGMIRQAKDVTL
                     VTRSERELMEIGQEFLRSKSRLMDKETALKTV"
BASE COUNT          297 a          279 c          300 g          224 t
ORIGIN      
        1 gcggacgcaa cgggcaaagc accagataca ctgctgatgc agctcgatac tcagttgcaa
       61 gctcagttgt cacaaaccaa gaagattccg cttgtcgaaa gagcggatga ccgatacctg
      121 gacgagctgt tctttggaac caaagacggc tgggttgacc ccgaacgtat tcccaaaaca
      181 gggaaacttg tcggtgcgaa gtactacctc tccacactgg taacgaagta ctccgcctcg
      241 gcttggggtg acaatgctgc gctgcccaat gtctatgaga acagctggag tgtttctgtt
      301 gaggtgacgg gcaaggtaat caatgttgag acgggacaac ttctcgaaac catactcgtc
      361 accgagtctt catcaggcaa gtctgaagac aaggaaaaga tgacttccgt gcccaatctc
      421 aatggcattc tgggttcact cgctcagaaa gcgacaggcg aaattgtaga ccagctgctg
      481 gtcattctct ttccggcgtt gattgccgac gtctctgaaa cggaaatctt cctcaatcag
      541 ggcgaagcca ccggtgtcaa agtgggagat cgttacacgg tcttctctca gggcaaagcc
      601 atcgtcgatc cacagaccgg aagaactctt ggaaaccgag agcagaaaat cggtgacatt
      661 gagatcgtcg aagttcgcag ggagtattcc acggcaaagt cggccgacgt ggcactgagt
      721 gagtttgccc caggcatggt ctgccgaccg gtcaccggag caaagaagtc cgaacctgtg
      781 gtgaaggatt cccagccaag cgtctcatcg tcaggtgcga catcacttgg tgatacgcca
      841 aagacatcga agcccaaaga ggaaactgct ccaaaggaac ccaaaggaag agtgttctgt
      901 ttggcggaaa tgctgaactc aacctcagcg ccaaggcgag ttgaacaggc catccatggt
      961 tacctttctg gcatgattcg acaggcgaaa gacgtcacac tggtcactcg ttccgagcgt
     1021 gaactgatgg aaattggcca ggaatttctg cgcagcaaga gccgtcttat ggacaaagag
     1081 acggctctga agacggtcaa
//
LOCUS       DAOQUC010001583         1100 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_196026_length_1100_cov_2.722488, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001583 DAOQUC010000000
VERSION     DAOQUC010001583.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1100
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_196026_length_1100_cov_2.722488"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..673
                     /gene="fabG"
                     /locus_tag="PKH07_20105"
     CDS             2..673
                     /gene="fabG"
                     /locus_tag="PKH07_20105"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HNT37307.1"
                     /translation="MRLAQEGARVVLSDVMEDALQATAEEFRSKGYTILAKKANVTEA
                     AEVTELVDAAVSEFGSLDILVNNAGITRDNLLLRMKEEDWDLVLKINLKGAFLCTKMA
                     LRPLMKSAGCIVNIASVIGIMGNAGQANYSASKAGLIGLTKSTAKELSSRGVRVNAVA
                     PGYIDTEMTRALPDNVREAILKNIPLARMGTPEDVANVVCFLAGDAAAYVTGQVIPVD
                     GGMVM"
     gene            677..>1100
                     /locus_tag="PKH07_20110"
     CDS             677..>1100
                     /locus_tag="PKH07_20110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNT37308.1"
                     /translation="MKENVRVVILAGGLGTRLKPYTTVFPKPLMPVGGRPILEIVIRQ
                     LQDQHFKEIVLSVGHLAELIEAYFLRAADMGLKVRISYEREEEPLGTAGPLRRIPGLD
                     QGTFLVLNGDLLVTGFDFRAFLESHKKSGAVLTIARFRR"
BASE COUNT          242 a          261 c          345 g          252 t
ORIGIN      
        1 cctgcgactt gctcaggaag gcgctcgggt ggttctttcc gacgtcatgg aagacgcact
       61 gcaggcaact gccgaggagt tccgctccaa gggatacacc attctggcca agaaggcaaa
      121 tgtgactgag gcagccgaag tgaccgagct ggttgacgct gctgtttccg agttcggttc
      181 gttggacatt ctcgtcaaca atgccggcat cacacgcgac aacttgttgc tacggatgaa
      241 ggaagaggac tgggatttgg tgttgaaaat caatctcaag ggagccttct tgtgcacaaa
      301 gatggcgctc aggccgttga tgaagtctgc aggctgtatt gtgaatatcg catcggtcat
      361 cggcatcatg ggcaacgctg gacaggccaa ctactctgcg agcaaggcag gtctgatcgg
      421 cttgaccaag agcacggcca aggaattatc ttctcgtggc gttcgggtga atgccgttgc
      481 tcccggctat attgatacgg agatgactcg ggcgttgcct gacaatgttc gggaagccat
      541 tttgaagaat ataccgcttg cgcgaatggg aacacccgaa gacgtggcaa acgtggtgtg
      601 ttttctggct ggagacgccg cggcatacgt cacgggtcag gtgattccgg tggatggcgg
      661 gatggtgatg tagcaagtga aggaaaatgt ccgcgtggtg atcctggcag gaggtttggg
      721 aactcggcta aagccgtata caaccgtatt ccccaagcct ctgatgccgg tcggcggtcg
      781 tccgattctg gagatcgtca tccggcagtt gcaggatcaa cacttcaagg aaatcgtgct
      841 ttcagtcggc catctggcag agctgataga ggcctatttc cttcgagctg cggatatggg
      901 actgaaggtg cgaatctcct atgagcgcga agaggaaccg ttgggcacgg ctggccctct
      961 tcgaaggatt cccggactag accagggaac ctttcttgtg ctaaatggag atcttcttgt
     1021 gacaggattt gacttcaggg cgttcctgga gtctcacaag aagagcggcg cggtgctgac
     1081 gattgcgcgg tttcgacgcg
//
LOCUS       DAOQUC010001584         1100 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_196105_length_1100_cov_2.193301, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001584 DAOQUC010000000
VERSION     DAOQUC010001584.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1100
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_196105_length_1100_cov_2.193301"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..486)
                     /locus_tag="PKH07_20115"
     CDS             complement(<1..486)
                     /locus_tag="PKH07_20115"
                     /inference="COORDINATES: protein motif:HMM:NF012475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HNT37309.1"
                     /translation="MADRLYREKYRPQFHFTAKGGWINDPNGLVFHEGEYHLFFQHVP
                     PDAPAGSLTWGHAVSTDLVHWSQLPNAIVPYDGGTIFSGSAVVDHNNSSGFQHGTEKA
                     LVAFFTHAREPFGQAAAYSVDRGRTWTLYSSKEYVVPNQGMDPGERDPRVFWHDPTRK
                     WV"
     gene            complement(499..>1100)
                     /locus_tag="PKH07_20120"
     CDS             complement(499..>1100)
                     /locus_tag="PKH07_20120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="Tat pathway signal protein"
                     /protein_id="HNT37310.1"
                     /translation="HDTWLGPYHRMISTPKYYEVCKNLIAEVTEIFDRPRFFHLGMDE
                     ETPQHQKHFDYVLVRQFDLWWHDFFSLVKQVEKNGARPWIWSDYVWWHPETFYERMPT
                     TVLQSNWYYRPEFTKKILAVRSYLDLEQHGYDQIPTGSNWTCQENFENTVKYLRRRID
                     RKRLMGFFMTVWRPSLEVCRERHMNALDLVAETRKHSRL"
BASE COUNT          241 a          296 c          290 g          273 t
ORIGIN      
        1 gacccatttt cgggtcgggt catgccagaa gactctcgga tcacgctctc caggatccat
       61 tccctggttc ggcacaacat attctttgct tgagtagagc gtccaagtgc gacctcggtc
      121 tacactgtag gccgcggctt gtccgaacgg ttcccgcgca tgggtgaaga aggccaccag
      181 agccttctcc gtaccatgct gaaaccctga ggagttgttg tgatccacca cggctgagcc
      241 tgagaagatc gttcccccat catacggcac gattgcgttt gggagttggc tccaatgcac
      301 cagatctgtg ctcacggcgt gtccccaagt cagactgcct gcaggagcat cgggtggcac
      361 atgttgaaaa aagaggtgat attctccctc gtggaacacc agaccgttgg ggtcattgat
      421 ccagccaccc ttcgcggtga agtgaaactg gggacggtac ttttctcgat agagtctgtc
      481 cgccatgaca ccatccatct acagtcggga atgcttacgg gtttctgcta cgagatcaag
      541 agcgttcatg tgtctttctc gacagacttc aagagatggt cgccacacgg tcatgaaaaa
      601 gcccatgagt cgcttgcgat caatgcgtcg tcgcagatat ttcaccgtgt tttcgaagtt
      661 ctcctggcat gtccagttgc ttcctgtggg aatctgatcg tagccatgct gttccagatc
      721 aagatagctg cgcacggcaa ggatcttctt ggtgaactct ggccgatagt accagttgct
      781 ctgaaggaca gttgttggca tcctctcgta gaatgtttcc ggatgccacc agacatagtc
      841 cgaccagatc cacgggcgcg cgccgttctt ttcaacctgc ttaaccaggg agaagaaatc
      901 gtgccaccac agatcgaatt gccttaccag gacgtagtcg aaatgtttct ggtgctgagg
      961 cgtctcttca tccatgccaa gatggaagaa tctcggtctg tcgaaaatct ccgtgacttc
     1021 cgcaatgaga ttcttgcaga cctcatagta tttcggcgtc gagatcatgc ggtgataggg
     1081 tcccagccag gtatcatggc
//
LOCUS       DAOQUC010001585         1098 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_196564_length_1098_cov_3.558965, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001585 DAOQUC010000000
VERSION     DAOQUC010001585.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1098
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_196564_length_1098_cov_3.558965"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="PKH07_20125"
     CDS             <1..181
                     /locus_tag="PKH07_20125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HNT37311.1"
                     /translation="IGKEKIGGGFVTVMVRGDVGAVKAATDAGAAAAKKVGEIVSVHV
                     IPRPHSDVDEILPKG"
     gene            212..874
                     /gene="pduL"
                     /locus_tag="PKH07_20130"
     CDS             212..874
                     /gene="pduL"
                     /locus_tag="PKH07_20130"
                     /EC_number="2.3.1.222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HNT37312.1"
                     /translation="MNTNLVQAITARVLEEIRGGGSSSGSTTPSRLLVPVGISVRHLH
                     ISQEDLETLFGHGAQLHRRNDLYQPGEYAAQESVTVIGPKLRAIERVRILGPTRPKTQ
                     VEISRTDAITLGVSPPVRDSGHLVNSESIVIVGPKGAIHLREGLILAGRHFHCPTDLA
                     TRHGLKDRQIVRIRIVGEKAMMLENALMRVRDSFKPEVHLDTDDGNAAGLVCGDLVEI
                     LP"
     gene            884..>1098
                     /locus_tag="PKH07_20135"
     CDS             884..>1098
                     /locus_tag="PKH07_20135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37313.1"
                     /translation="MSIDPKRVAQIVREEIEQYLKGRTFPVASPVSQASPIQESVSFK
                     ESLLAIFLGSTRGLDSVTLALRNLARSG"
BASE COUNT          238 a          285 c          321 g          254 t
ORIGIN      
        1 gattggcaag gaaaagatcg gtggcggttt tgtaaccgtc atggttcgcg gcgacgttgg
       61 tgcggtgaaa gccgcgactg acgctggcgc ggcggctgcc aagaaggttg gcgagatcgt
      121 atccgtccac gtgattccga gaccgcacag cgatgtggac gagattctgc cgaagggcta
      181 gagcagtctg aagcactaca cgagttgacg catgaacaca aacctcgttc aggcgattac
      241 ggctcgcgtg ttggaagaga ttcggggtgg tggctcctcg tcaggatcta ctaccccatc
      301 ccgtcttctg gtgccggtgg gaatttctgt tcgccatctg cacatcagcc aggaagatct
      361 ggaaacactc ttcggacatg gagcccaatt gcatcgtcgc aatgatttgt accagcctgg
      421 ggaatacgcg gctcaggagt ctgtgaccgt tatcggtccc aaactccgtg ccattgagcg
      481 cgtgcgcatt cttggtccga cgcgtccaaa gacccaggtc gagatctccc gaacagatgc
      541 catcactttg ggtgtgtctc ctccggtgag agattccggc catctggtca actctgagtc
      601 tatcgtcatc gtgggtccca aaggggcaat ccatctacgc gaagggctta tccttgcggg
      661 acgccatttt cactgcccca cggatctcgc tacacgacac ggtctgaaag acagacagat
      721 cgttcggatt cgcattgtgg gcgagaaggc catgatgctt gaaaacgcac tgatgcgcgt
      781 tcgggacagt ttcaagcctg aagtgcatct agacaccgat gatggcaacg ctgcaggctt
      841 ggtctgcggt gatctagttg agattctacc ataggtcaac gaaatgagca ttgatccaaa
      901 acgtgtggca cagattgttc gggaagaaat cgaacagtat ctgaagggaa ggacattccc
      961 tgttgcctct ccggtttccc aggcgtctcc gatacaggag agtgtttcct tcaaggaaag
     1021 cctgctggcg atctttcttg gcagcacgcg aggcttggat tccgtgactc tggcacttcg
     1081 gaatcttgct cgttcggg
//
LOCUS       DAOQUC010001586         1098 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_196577_length_1098_cov_3.190796, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001586 DAOQUC010000000
VERSION     DAOQUC010001586.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1098
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_196577_length_1098_cov_3.190796"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1098)
                     /locus_tag="PKH07_20140"
     CDS             complement(<1..>1098)
                     /locus_tag="PKH07_20140"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNT37314.1"
                     /translation="AHDFNNILSPILGYSQLAWYDLPQDSPVRAYLDHVLDAGKRAQA
                     LVRQFQEFSGKAVFELQPMAIQPVVDECLVALLDAVPSNVEIAAVMDSQCGLVLANEA
                     PIREVVMNLCDNALDAMKDTAQTPAEKQEKCVLRIELGEIEVTPDKSASFQDLKAGRY
                     VRFVVSDTGRGIDEETRQHLFEPYFTTKPKGEGTRGLGLAVVYGIVKSLDGAIVVQSE
                     LGAGSRFEVFLPVCAQPKREKDVCQEERILGGTGRLLFVDDEESILEVSKEFLERIGY
                     QVTTCISPVEALRIFRETPEEFDLVMTDQTMPAMTGIELATALSGIRKDIPIVLCTGF
                     GETLSREEARSRGISQFMEKPVVLPDLAREVR"
BASE COUNT          256 a          326 c          270 g          246 t
ORIGIN      
        1 acgcacctct cgagccaagt cgggaaggac gactggcttt tccatgaact gactgattcc
       61 tctgcttctg gcttcttccc gagagagcgt ctctccaaac cccgtgcaaa ggacaatggg
      121 gatgtccttt cgaataccgc tcagcgcggt ggccaactca atgcccgtca tcgcaggcat
      181 ggtctgatcg gtcatgacga ggtcgaactc ttccggtgtt tctctgaaaa tcctcagtgc
      241 ctcaacggga ctgatacacg tggtcacctg atagccaatt cgttccagaa attccttgct
      301 gacctcaaga atggactctt cgtcatccac aaagagcaga cgacctgtcc cccccaggat
      361 tctttcttcc tggcatacat ccttctccct tttgggctgt gcgcaaacgg gaaggaaaac
      421 ttcgaatcgg gaacctgccc ctagctcgct ctgaacgaca atcgctccgt caaggctctt
      481 aacgatacca tagacaaccg ccaaaccaag tccgcgagtt ccttccccct tgggcttcgt
      541 tgtgaagtaa ggttcgaaca ggtgctgtcg tgtttcctca tctatcccac ggccggtatc
      601 tgaaaccaca aaacggacat agcggccggc tttcagatcc tgaaacgacg cggatttgtc
      661 cggcgtgacc tcgatctcac ctagttcgat ccgcaagaca cacttctcct gcttttccgc
      721 tggagtctgc gccgtgtcct tcatcgcatc gagtgcgttg tcgcagagat tcatcaccac
      781 ttcacgaatc ggagcctcat tggccaagac caaaccacat tgggagtcca tcaccgcagc
      841 gatctcaacg ttggagggga cggcgtcgag cagcgcaaca agacactcat ccacaacggg
      901 ctgaatggcc atcggctgca gttcaaagac agccttcccg ctgaactcct gaaattgacg
      961 aaccagcgct tgagcgcgct tgccagcatc gaggacatga tcaagatagg ctcgtacagg
     1021 ggaatcctgt ggcagatcat accaggccag ttgggaatag ccgagaatgg gactgagaat
     1081 gttgttgaaa tcatgggc
//
LOCUS       DAOQUC010001587         1097 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_196995_length_1097_cov_2.382917, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001587 DAOQUC010000000
VERSION     DAOQUC010001587.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1097
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_196995_length_1097_cov_2.382917"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="PKH07_20145"
     CDS             complement(<1..282)
                     /locus_tag="PKH07_20145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37315.1"
                     /translation="MMKKVSQFSSRNGKIEGIVLGVLLVLMLISNRVRSDTGLVGRGL
                     YPEEIDVSNRISMNLDSIEADKMIADLLSGELKGLHHIGTIVEYDSTKKE"
     gene            complement(296..>1097)
                     /locus_tag="PKH07_20150"
     CDS             complement(296..>1097)
                     /locus_tag="PKH07_20150"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HNT37316.1"
                     /translation="IGAPMKEVVYEYASNASSVPSATDSLYYHTDHISNVVGLTNAGG
                     ISSADSGYFEQDAFGNEMNGTWGSFSRHLTAKEYDADCEMYFFSARWLEPRLGRFPSS
                     DPIGFVTNYAYAMNCPTLLIDPTGLYEAIPCDPGKVCGEWTTGVKYGDRYIRTDTKVL
                     VPWHLVSMHFTLIDAGPPAGSLTCVAQRVLRKTEIREKYKVTTRSRICSQGNTIFVET
                     GIPKEESLGYTRKTWKETEEQTVTVLTPPWLDWVRFERICSNFVSSLR"
BASE COUNT          272 a          290 c          247 g          288 t
ORIGIN      
        1 ctccttttta gtggaatcat attcaacaat tgtacctata tgatgaagtc ccttaagctc
       61 gcctgaaaga agatctgcaa tcatcttgtc ggcttcaata gagtcgagat tcatggaaat
      121 gcgattggag acatcaatct cctcgggata cagaccccta ccaactaaac cagtatcaga
      181 tcttaccctg ttggatatga gcatcagaac gagaaggacg cccaaaacaa tcccttctat
      241 cttcccattt ctcgaggaaa actgagacac tttcttcatc accaatctcc ttcttctaac
      301 gcaggctgct tacaaaattg ctgcatatcc tctcgaatct cacccaatca agccaagggg
      361 gtgtaaggac agtgacagtc tgctcctccg tctctttcca ggttttgcgg gtgtacccaa
      421 ggctctcctc tttgggtatt ccggtttcaa caaatatcgt attaccctgc gagcagatcc
      481 gacttctggt agtcactttg tatttctcgc gtatttctgt tttgcgaagt acgcgttgtg
      541 caacacaagt aagcgaacct gcaggtgggc cagcatctat aagtgtaaag tgcatgctga
      601 ctaagtgcca aggtaccaga acctttgtgt cggtacgaat atacctatcc ccgtacttga
      661 ctcccgttgt ccactccccg caaaccttgc ccggatcaca cgggatcgcc tcgtagagtc
      721 cggttggatc gatgaggagg gtgggacagt tcatagcata ggcgtagttt gtgacaaaac
      781 ctattggatc agaagacggg aaccttccga gcctcggctc aagccaacgt gcgctgaaga
      841 agtacatctc gcagtctgca tcatattcct ttgcggtcag atgccgggag aatgagcccc
      901 aagtaccgtt catctcgttt ccgaaggcgt cctgctcgaa gtacccactg tctgctgatg
      961 aaatcccgcc agcattcgtc agaccaacca cattgctgat atgatcggtg tgatagtaga
     1021 gactgtccgt cgctgaagga actgaagacg cattgctcgc gtattcgtag acgacttcct
     1081 tcattggtgc gccgatc
//
LOCUS       DAOQUC010001588         1095 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_197578_length_1095_cov_2.774038, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001588 DAOQUC010000000
VERSION     DAOQUC010001588.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1095
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_197578_length_1095_cov_2.774038"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..954
                     /locus_tag="PKH07_20155"
     CDS             <1..954
                     /locus_tag="PKH07_20155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37317.1"
                     /translation="EIGNEPELGGIGTYIQNHYVSPTDFKVRYKAIAEAMKAVDPSVK
                     LGPCTLTPNNTTTQEYLAHLLSDPEIPVNFLGFHPYYGTLYYVYLYNWGSPDVLEQLE
                     SGLRGLKTYQENVASTILGFSASNGFDTSIPWIASEWNSSDWRGTWTPLGKCMTHALG
                     VAEGIFVFANLGYLGAHYWEQPSVGPQGYKMFEVLQDHMGDHLINSYTDGYNFRLYTT
                     RFRNWSKIVLWGINFSDSENKELDIGFTHFPFSVRRVTKMQLGVVGADSGLKSSAGVN
                     WTSTDITGIDLRDFSVTFEDATVTAIVIETEGASVRWLDYD"
BASE COUNT          270 a          283 c          274 g          268 t
ORIGIN      
        1 gagatcggca atgagcctga actgggaggc atcggaacgt atattcagaa ccactatgtc
       61 agcccgacag acttcaaggt gcgctataaa gcaattgccg aggcgatgaa agcagtggat
      121 ccttctgtca agttagggcc gtgcaccttg actcccaaca acacaaccac tcaggaatac
      181 cttgcccatc ttctgtctga tccagagatc cctgtgaact tccttggttt tcatccatac
      241 tatgggactc tctattacgt atacctttac aactggggga gtcccgacgt cttggagcaa
      301 ctggagagcg ggcttcgagg gctgaaaacc tatcaggaga acgttgccag caccattctt
      361 ggcttttctg cgtccaatgg cttcgacaca tctattccat ggattgcaag cgaatggaac
      421 tccagcgact ggcgaggaac atggacgcca ttgggtaaat gtatgaccca tgctcttgga
      481 gtggcggaag gaatctttgt gtttgcaaac cttgggtatc tcggggcgca ctattgggag
      541 cagccttcag tgggcccaca ggggtacaag atgttcgaag ttctacagga tcacatggga
      601 gatcatctca tcaacagtta caccgacggg tacaatttcc gtctctacac aacacgattc
      661 aggaactgga gcaagatcgt cctctgggga atcaacttct cggattcaga aaacaaggaa
      721 ctggacatcg gtttcacgca cttcccattt tccgtccgaa gagtaaccaa gatgcaactt
      781 ggtgtcgttg gcgcagatag cggtctcaag agttcagccg gagtcaactg gacgtctaca
      841 gacatcacag gaattgacct tcgcgacttt tccgtcactt tcgaggatgc aacggtcacc
      901 gccatcgtca ttgagacgga aggcgcgagt gttcgctggc tggactatga ctgatgctgt
      961 tggcacctca gacgcctcag agcatccctg atttcctgag cctctgttct ggagtattcc
     1021 cacagacgaa cttgcgcaat tgccgcattc catcttcgcc ttgacagact gcagtacaga
     1081 atctattttg tcatt
//
LOCUS       DAOQUC010001589         1095 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_197608_length_1095_cov_2.540385, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001589 DAOQUC010000000
VERSION     DAOQUC010001589.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1095
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_197608_length_1095_cov_2.540385"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            150..908
                     /locus_tag="PKH07_20160"
     CDS             150..908
                     /locus_tag="PKH07_20160"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNT37318.1"
                     /translation="MDTSTPSLSERLEYLQTIFLLIESSLKQNKTPELSPENWGEMRD
                     ALGAVLQITQRPGSLQERLQQVEKERDVVRELLEVTNLRMEQQVDQLSLLRLIYDVVA
                     QAVLLNDPFRFLLQQIVTVTNAENGSIMLLDESKEKLILQAAAGTKDFAPQGVCFNVG
                     EGIAGLVAQRGQAQVISDTLKDPIYKGLGHHQESIKSLICMPLICHREILGVLNLSSS
                     RVDAFSQNMYRFLQVVAGYLAMLVIETRENTSEA"
BASE COUNT          266 a          272 c          299 g          258 t
ORIGIN      
        1 gacgatccgc ccgagagcag tcacctgagt gagagccttc aggggctggg actcgatgag
       61 gagagacttg aaagcctcat gggtaccctg agagaaggat tcaagaaact ggaggctttc
      121 ctggcagcca actagaggag agcattgaga tggacacttc gacacccagt ctatccgaaa
      181 gactcgaata cttgcagacg atctttttgc tgattgagtc atctctcaag cagaacaaga
      241 ctccggagct gtctccagag aactggggtg agatgagaga tgctctgggt gccgtgctgc
      301 agatcactca aagaccgggt tcactccagg aacgtctgca gcaagtggag aaggaacggg
      361 atgtcgttcg cgaactgctc gaagtgacca accttcggat ggagcagcag gtggatcagt
      421 tgtcactgct gcgtctgatc tacgatgttg tggcgcaggc tgtactgctc aacgatccct
      481 ttcgttttct gcttcagcag attgtgaccg tcacaaacgc cgagaatggt tccatcatgc
      541 tcctggatga gagcaaggaa aaactgattc tgcaggcggc agcgggaacc aaagacttcg
      601 ctcctcaagg cgtgtgtttc aacgttgggg aaggcattgc cggactggtc gcacaacgag
      661 gtcaggcaca agtcatctcg gacacgctca aagatcccat ctataaaggc ttgggacacc
      721 atcaggaatc catcaagtcc ctgatctgta tgccactgat ctgccatcgg gaaattctcg
      781 gtgtgctgaa tctgagtagt tcccgggtgg atgcctttag ccagaacatg taccgctttc
      841 tacaagtggt cgccggatat cttgccatgc tggttataga gacacgcgaa aacacatcag
      901 aggcgtaaac cgatatcctt ttcctctact ggctgcggag tccagtcctg tccttgcatt
      961 cagatcttct acaaagaaaa acctctcgtg aagagaggtt tatttgccgg ttattttgaa
     1021 caagcctctt gcgagggtgc tcttgctctg ggtcggctgc ccttcgcgtc tttccttgtt
     1081 atggaaagaa ccgca
//
LOCUS       DAOQUC010001590         1094 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_197831_length_1094_cov_3.259865, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001590 DAOQUC010000000
VERSION     DAOQUC010001590.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1094
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_197831_length_1094_cov_3.259865"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1094)
                     /locus_tag="PKH07_20165"
     CDS             complement(<1..>1094)
                     /locus_tag="PKH07_20165"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HNT37319.1"
                     /translation="IARLILAASPEEVQEYLTRILSLDLSESQRAQASQLFGLMDTMQ
                     QYLTDWEVSGVYESDGKGASLLFDIAFDPECVTGAPVIWKPMQAGLNPDAPWFMDLEK
                     SLGAANNRVAYLRTELVKSESGRIMLEAGSDDGIKVWVNGELVLVNNAIRGYSPDTDK
                     VPVQLKQGTNEILLKVTQEHSGWGASLRLRELDEHGALKDVVPPVESPLIFRRIKISD
                     ERFEAASSFDVNKDGHLDIVSGEYWYQGSGFKKRHKICDVKPEGEYYDDFGDFPMDVN
                     GDGYTDIVTGGFWGCTVRWRENPKGRTTLWRVHDVDVCGNIETLRCWDVDGDGCPEVV
                     PNAGGNIVAYRLIRDQEGKGTGSFEKHVIK"
BASE COUNT          252 a          331 c          253 g          258 t
ORIGIN      
        1 tgcttgatga catgcttctc aaaagagccc gtgcccttgc cctcttgatc gcggataagc
       61 ctgtacgcaa caatgttccc gccggcgttt ggaaccacct cgggacatcc gtcgccgtcc
      121 acatcccaac atcgcagtgt ctcgatgttg ccacacacgt ccacatcatg aacgcgccag
      181 agtgtggtgc gtcccttcgg attctcgcgc cagcgcacgg tgcagcccca gaaaccacca
      241 gtcacaatat ccgtgtatcc gtcaccgttg acatccatcg ggaaatcgcc aaagtcgtcg
      301 tagtactcac cttccggttt gacgtcacag attttgtgtc tctttttgaa gccggagcct
      361 tgataccagt actctccgga gacgatatca agatggccat ccttgttgac atcaaatgat
      421 gatgccgcct caaatcgctc atcagagatt ttgatgcgac gaaagatcag aggggattct
      481 acgggtggca caacatcctt cagcgcacca tgttcatcca actctcgcaa acgcaacgag
      541 gctccccaac cggaatgctc ctgggttacc ttgaggagaa tctcattggt gccctgtttt
      601 aactgtaccg gcaccttgtc tgtatcaggg ctgtatcctc gaattgcatt gttcaccagc
      661 accaactctc cattgaccca cactttgatg ccatcgtcac ttccagcttc cagcatgatc
      721 cgaccacttt cacttttgac caactcagtg cggagatagg ccactcggtt attcgcagca
      781 cccaatgact tttccaagtc catgaaccaa ggtgcgtcag gattgagccc agcctgcatc
      841 ggcttccaga tgacgggggc tcccgtcacg cactcgggat caaaagcgat atcaaagaga
      901 agtgatgccc cttttccatc gctttcgtac acgccggaga cttcccagtc ggtgagatac
      961 tgctgcatcg tgtccataag accgaacaac tggctggcct gagcccgttg tgactcggac
     1021 aagtccagac tcagaatgcg agtgagatac tcctgaactt cttccgggct ggcagcaaga
     1081 atcagtcgag cgat
//
LOCUS       DAOQUC010001591         1093 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_198170_length_1093_cov_2.933526, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001591 DAOQUC010000000
VERSION     DAOQUC010001591.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1093
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_198170_length_1093_cov_2.933526"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(111..>1093)
                     /locus_tag="PKH07_20170"
     CDS             complement(111..>1093)
                     /locus_tag="PKH07_20170"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HNT37320.1"
                     /translation="GVVLEGQIPSYTMPSVKNAAGYYADDDMDLVDLFIGSEGTLGIV
                     SEVELELIRVPSCVWGLMAFFESEGKAVAFVRKVREAAPPHPDSHSVARLAAVEFFSG
                     GALRLLRDAITTNAAFASIPSPDLRHQTAIYLEIHGNDEDCVGDLMMTASEAMVECGG
                     KEEDTWMASESHEIQRLKDFRHAVPEAVNLVIDQRRKAEPKLVKLGTDMSVPDDHLER
                     VLSLYNSQLIEANLEYVIFGHIGNNHLHVNILPRSLEEYEKGRELYLNWAHEIVELGG
                     SISAEHGVGKLKTNLLKTMYGANGIEEMRSVKSVFDPHNRLNPGNLFERI"
BASE COUNT          258 a          291 c          268 g          276 t
ORIGIN      
        1 gctgacgaag ggaatgctcg acaagtagaa actgaagatg gatagatggc tcatgtcgtg
       61 gggcattcct gaagagtgcc ccacttcact ttttagggtt ctttccagaa tcagattctc
      121 tcaaaaagat tacctggatt gagtctgttg tgaggatcga aaacgctctt gaccgatctc
      181 atctcttcga ttccgtttgc gccatacatc gtcttaagaa gatttgtctt gagttttcca
      241 accccgtgtt ctgcagagat gcttccacct aattccacga tttcatgcgc ccaattgagg
      301 tagagttccc tgcccttctc atattcctca agagatctcg ggagaatgtt gacgtgaagg
      361 tgattgttgc cgatatgacc aaagatgaca tattcaagat tggcctctat caactggcta
      421 ttgtagagac tgagaactcg ttccagatga tcgtcaggta ccgacatgtc cgtgccaagt
      481 ttgaccagct tcggctctgc cttccgccgc tgatctatga ccagattgac ggcctcaggc
      541 accgcatggc ggaaatcttt gagcctctgg atttcgtgag actcgcttgc catccaggtg
      601 tcctcttcct tgcccccgca ctcgaccatt gcttccgatg cagtcatcat gagatcgccg
      661 acgcagtctt catcgtttcc atggatctca agatagatcg ccgtttgatg cctcagatcg
      721 ggagagggga tggatgcaaa ggcggcgttg gtggtgatcg cgtctcgaag aagtctcaaa
      781 gcaccgccac tgaagaactc gacggcagcc aggcgagcca cagaatggga atcaggatgg
      841 gggggggctg cttcacgaac tttcctcaca aaggcaaccg ctttgccctc tgactcaaag
      901 aaggccatga ggccccagac acaacttggg actcgaatca gttccagttc aacttccgag
      961 acaatgccaa gtgtcccttc acttccgatg aacagatcaa caagatccat gtcatcgtcg
     1021 gcgtagtatc ccgctgcatt cttcaccgag ggcatcgtat aacttgggat ctgcccttcc
     1081 agaacaacgc ctg
//
LOCUS       DAOQUC010001592         1091 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_198759_length_1091_cov_2.710425, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001592 DAOQUC010000000
VERSION     DAOQUC010001592.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_198759_length_1091_cov_2.710425"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..721)
                     /locus_tag="PKH07_20175"
     CDS             complement(<1..721)
                     /locus_tag="PKH07_20175"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HNT37321.1"
                     /translation="MPGSAFSDDYQVSLLLSRNSVNVGEQFYLQVEVKGTSRDIPSPS
                     LPDVDGLDFFSGGTTSSFQIINGNVQSSKTFAYSVFARKEGTYTLDSVQVEIGGKVYK
                     AQPATITVSAPAGQPGQGAPQPGQPHASQGQPSPQETPQSPPTESIMLKAIPENTTVY
                     ANQGIVLQYILYKHPQIQLGGNIQFENLQSTLFQGFWQEEIPMQPRSVGTQNIGNAPY
                     DVIELKKYVLFPLKPEAVQIEP"
     gene            complement(762..>1091)
                     /locus_tag="PKH07_20180"
     CDS             complement(762..>1091)
                     /locus_tag="PKH07_20180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37322.1"
                     /translation="KQEEQPTPEQTQTNDDQAQQTPTPQEGEQQQSEESTPTPQQLPE
                     PSTETPTPSDAQQANEGDQSAPTETPTPVDREQFERLLDRLDQKELEIRKKLQGTPPP
                     IHVEKDW"
BASE COUNT          218 a          254 c          314 g          305 t
ORIGIN      
        1 ccggttcgat ctgcaccgcc tccggtttga ggggaaacag aacgtacttc ttgagttcga
       61 tgacgtcata aggagcgttg ccgatgttct gagtgccaac gcttctgggc tgcatcggaa
      121 tctcctcttg ccagaagcct tgaaagaggg tggactgaag attctcgaac tgaatatttc
      181 cacccagctg aatctgagga tgtttgtaaa gaatatactg aaggacgatt ccctgattcg
      241 cgtagactgt cgtgttttct ggaatcgcct tgagcatgat tgattcggtt ggaggtgatt
      301 gaggggtctc ctgtggagaa ggttgaccct gtgatgcgtg aggctgtccg ggctgtggtg
      361 ctccttggcc gggttgtcca gccggagcgc tgaccgtgat cgttgcgggc tgtgccttgt
      421 agacttttcc gccaatctcg acctgcactg aatcaagggt ataggttcct tccttgcggg
      481 cgaaaacgga gtaggcgaag gtcttgctgg actgcacatt gccattgata atctgaaatg
      541 aagaggtcgt gcctcccgag aagaagtcca gaccgtccac gtcgggaaga cttggagaag
      601 ggatatctcg tgacgttccc ttaacttcga cttgaagata gaactgctcg cccacattga
      661 ccgagttgcg actcagcagc agtgaaacct ggtagtcatc cgaaaaagcc gaaccaggca
      721 tcaggatgac tgccagaatc cacagagaga ataaacttgt tctaccagtc tttttcaaca
      781 tgaatcggtg gcggagttcc ctgtaatttc tttctaatct cgagttcttt ctgatcaagc
      841 ctgtccaaca ggcgttcaaa ttgctcacga tctacgggag ttggtgtttc agtcggtgcg
      901 ctctgatcac cctcattggc ttgctgggca tcagatggtg tgggggtctc tgtggatggc
      961 tcaggcagct gttgaggcgt cggcgttgat tcctccgact gctgctgttc tccttcctga
     1021 ggggttggcg tctgttgtgc ctgatcgtcg tttgtctgtg tctgttcagg agttggttgt
     1081 tcttcttgct t
//
LOCUS       DAOQUC010001593         1091 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_198777_length_1091_cov_2.619691, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001593 DAOQUC010000000
VERSION     DAOQUC010001593.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_198777_length_1091_cov_2.619691"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="PKH07_20185"
     CDS             complement(<1..196)
                     /locus_tag="PKH07_20185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37323.1"
                     /translation="MKLKDRWLFYVDIIGGIALTLFVLWVFVFNPGRIGRPNVVLILV
                     DALRADYLGCYGHRTPTSPTL"
     gene            complement(200..>1091)
                     /locus_tag="PKH07_20190"
     CDS             complement(200..>1091)
                     /locus_tag="PKH07_20190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013150831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TSCPD domain-containing protein"
                     /protein_id="HNT37324.1"
                     /translation="VLDGEKLVEFHPRFLQRAKREGFHSDALLERVAELGSVQGLKEI
                     PRVVRRVFVTAHDISPDWHVKMQAAFQKYTDNAVSKTVNFPTQSTREDVAHVYKLAFK
                     HRCKGVTIYRHGSRDEQVLNLGAPKEGEQPSPRVPRPRQPLTRGVTEKIRIGCGNLYV
                     TVNADNKGICEVFTNLGRAGGCPSQSEATSRLISLALRSGVDVSAIIEQLRGIRCLST
                     VARKGREGLTVLSCPDAIGRALVRSQIQMETESPQPPPVESNEPLTAATTEACPDCGS
                     NVEFDGGCMVCTSCGYSKCG"
BASE COUNT          252 a          322 c          282 g          235 t
ORIGIN      
        1 cgagcgtcgg tgaagtcgga gttcgatgac cgtagcaacc gagatagtcc gcccggagcg
       61 catccaccag gatcagcact acgttcggcc tgccaatgcg cccagggttg aagacaaaga
      121 cccagagcac aaaaagcgtc agggcgatgc ccccgatgat gtcaacgtag aacaaccaac
      181 gatccttcaa cttcatcgtc tacccacatt ttgaatagcc acaggaagtg cagaccatgc
      241 agcctccgtc aaactcaacg ttggagccgc agtcgggaca ggcttcggtg gtcgccgcgg
      301 tcagcggttc gtttgactct actggcggag gttgcggtga ctctgtttcc atctgaatct
      361 gagagcgtac caatgcgcgt ccgatggcat caggacagga gagcaccgtc agtccttcac
      421 gcccctttcg agccacagtc gaaagacacc gaatccctcg cagctgttca atgatcgccg
      481 agacatcaac tcccgaccgc agagcaagcg agatcaagcg gctggtggct tcagactgtg
      541 aaggacaccc accagcgcga cccagattgg tgaacacttc gcagatgcct ttgttgtccg
      601 cattgacagt cacatacaga ttcccacatc caattcggat cttctcggtt acaccacggg
      661 tcaatggctg acggggacga gggactctgg gagatggttg ctcgccttct ttgggagcgc
      721 caaggttcaa cacttgttca tcgcggcttc cgtgccgata gatcgtcact cccttgcatc
      781 tgtgcttgaa tgcaagtttg tacacatgcg ctacatcctc acgtgttgac tgtgttggga
      841 agttgacggt cttcgatacg gcattgtcgg tgtatttctg aaaggccgcc tgcattttga
      901 catgccaatc gggagaaata tcatgggctg ttacaaacac tcttctcacg acacggggga
      961 tctctttcag cccctgaaca ctgcccaact ccgcaacccg ctccaaaagc gcgtcgctgt
     1021 ggaaaccttc ccgcttggca cgctgcaaaa atcggggatg aaactccacc agtttctctc
     1081 cgtccagaac c
//
LOCUS       DAOQUC010001594         1091 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_198789_length_1091_cov_2.547297, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001594 DAOQUC010000000
VERSION     DAOQUC010001594.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_198789_length_1091_cov_2.547297"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..403)
                     /locus_tag="PKH07_20195"
     CDS             complement(<1..403)
                     /locus_tag="PKH07_20195"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNT37325.1"
                     /translation="MSRLCVKTILSELVACPSVNPRRLVPTDSPFGEERLQNLVAGWL
                     SEWGAKTEMQSVTEGRCNLIARFRGLDSSRSLMLEVHADTVQADDMTIAPFEPQIREG
                     RLYGRGSCDDKGPMAAVLAAIKTVLDEEGMPP"
     gene            complement(423..>1091)
                     /locus_tag="PKH07_20200"
     CDS             complement(423..>1091)
                     /locus_tag="PKH07_20200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002644717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HNT37326.1"
                     /translation="IHHLGQGVLSDMGAVYTLGPSPGTTVSNNVIHDVYAFSYGGWGL
                     YNDEGSTGILMENNLVYNVKTGTYHQHYGRENVLRNNILAFSESGQIQRTRAEEHLSF
                     TIENNIILWKTSPLLTGNWSGTNYILKNNLYCNISGEPISFAGKTFEQWQQDGHDAGS
                     GIADPGFVDVENRDFRFEGESPAFSIGFKPFDFDRAGVYGDPKWVDKPKGFVHRPRVS
                     FDAR"
BASE COUNT          255 a          307 c          261 g          268 t
ORIGIN      
        1 cgggaggcat gccctcttca tccagaacgg tcttgatggc tgcaagcact gctgccatcg
       61 gccccttgtc atcgcaggaa cctcttccat agagacgccc ttcccgaatc tgcggttcaa
      121 atggtgcaat ggtcatgtcg tctgcctgaa ccgtgtccgc atggacttcc agcatcaacg
      181 aacggctgga gtccagtccg cgaaaacgtg cgatcaaatt gcatcgtcct tccgtcacgg
      241 attgcatctc tgtcttcgca ccccactctg agagccagcc ggcaacaaga ttctgaagtc
      301 tttcctcacc gaaaggggaa tctgtcggaa caagtcttct cggattgacg cttggacaag
      361 cgacaagttc cgaaaggatg gtcttcacgc aaagacggct catatctacc tctccttatc
      421 ttttatcggg cgtcaaaaga cactctcggt ctgtgcacaa agcctttcgg cttgtccacc
      481 cacttaggat cgccatagac tcccgcccgg tcgaagtcaa acggcttaaa gccaatggaa
      541 aaagcaggtg actccccttc aaatcgaaag tcccgattct ccacatcaac aaaacccgga
      601 tcggcaatgc cggagccagc atcgtgtccg tcctgttgcc attgctcaaa cgtcttcccg
      661 gcaaaagaga ttggctctcc ggagatgttg cagtacaggt tatttttgag aatgtagttt
      721 gtgccgctcc agttgcctgt caacaacgga ctcgtcttcc acaagatgat attgttctcg
      781 atggtgaagg agagatgttc ttcggcgcga gtgcgctgaa tctgaccgga ctcgctgaag
      841 gcaaggatgt tgtttcgcag cacattctct cttccatagt gttgatggta ggttccagtc
      901 ttgacgttgt agacaaggtt gttctccatc aggatgcccg tgctgccttc atcgttgtag
      961 agtccccaac caccgtatga aaaggcatag acatcatgaa tgacattgtt gctgaccgtc
     1021 gtgccagggg acggacccaa tgtgtatacc gcacccatat cactcaacac accttgtccc
     1081 agatgatgaa t
//
LOCUS       DAOQUC010001595         1090 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_199103_length_1090_cov_2.623188, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001595 DAOQUC010000000
VERSION     DAOQUC010001595.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1090
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_199103_length_1090_cov_2.623188"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..512
                     /locus_tag="PKH07_20205"
     CDS             <1..512
                     /locus_tag="PKH07_20205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37327.1"
                     /translation="RRLADIADFTLRLQFNVTKCQVAPSLTVNPSLHTAQISAYWTVH
                     DIDEQGRSRDMIWLGVPLFDARFDIPPPHHALDVSKDDATLKFIYLIDGHEFWDEPIR
                     VGKWHYLERDIKPFLEGALSKSKKEGHLLDASMDSLALTSFNLGWEVPGPYDCTVEIR
                     RLDVVTTKR"
     gene            673..882
                     /locus_tag="PKH07_20210"
     CDS             673..882
                     /locus_tag="PKH07_20210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37328.1"
                     /translation="MSQEHNTISCTTTCRTEPELRAWRKRLLRRAMFLCILGIPIGLL
                     MKLPAVWILGAIGVVVGWVKLKQMG"
BASE COUNT          262 a          257 c          300 g          271 t
ORIGIN      
        1 cccgccggct tgcggatatc gcggacttca cgcttcgtct gcagttcaac gtgacaaagt
       61 gccaggtcgc accctctctg accgtgaatc caagtctgca cacggcgcag atttccgctt
      121 attggactgt gcacgacatt gatgagcagg gtcgttcccg ggatatgatc tggctgggag
      181 ttcctctgtt tgatgctcgt ttcgatattc caccgcctca tcatgccctt gatgttagta
      241 aggacgatgc cacgctcaag ttcatctatc tcattgacgg acatgagttc tgggatgaac
      301 caattcgagt gggcaaatgg cattatttgg aacgcgacat caagcctttt ctcgaaggtg
      361 cactcagcaa atcgaagaag gaaggacatt tgctggatgc aagcatggat tccctggcac
      421 tgacatcttt caatctaggg tgggaggttc cgggtcccta cgattgtacg gttgaaatcc
      481 gccgattgga tgttgttacg acgaaacggt agcactcatg atacagcgtg catcctttcg
      541 gacgccctga ggatctaagg ctgatgccga gatgacccgg ctccggcaga aacgcaattt
      601 gcagaagaga agtgaatatc ttctgtactg ccgtgtagta aggatcagac tgcaattgaa
      661 gaggagatct caatgtcaca ggagcacaac accatttcct gcacaaccac ttgtcgtacg
      721 gagccggaac tccgagcgtg gagaaagaga ctgctgagac gtgcgatgtt tctctgcatt
      781 ttaggcatcc caattggctt gttgatgaaa ctgccggcag tctggattct gggggcgatc
      841 ggggttgtgg ttggatgggt gaaactcaaa cagatgggtt gaagtgggtt gcgatgatgc
      901 tgctgaatcc ctgaaaagga acgaaactcc tcttccgagg gatttgcgat agatatagta
      961 cagtgccaag tgtcattctg aacggagcaa agcggagtga agaatctctc tgattgcacc
     1021 agccttatgt tgggaagaga gattcctcac tccttcgcca agcctcagtc gttcggaatg
     1081 acagtctccg
//
LOCUS       DAOQUC010001596         1090 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_199153_length_1090_cov_2.302415, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001596 DAOQUC010000000
VERSION     DAOQUC010001596.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1090
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_199153_length_1090_cov_2.302415"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1090)
                     /locus_tag="PKH07_20215"
     CDS             complement(<1..>1090)
                     /locus_tag="PKH07_20215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="RND transporter"
                     /protein_id="HNT37329.1"
                     /translation="TILRNTGRFTYKNSKLVLAFVVLCVVVAGYGISQIQINDNPTRW
                     FAPSHPIRVADRVLNSHFGGTYTAYLALEPSDEHSDLPEYVSDLGNRLYDKGTELESS
                     YPGSHEIFRIVREKLSMLVASVQAKHQLLDALSMDVNSQLDTAPDELYDAWDQASLFL
                     EIEKQRDEVFKQPEALAYISRLQEALLETGIVGKSNSLSDIVKTVYRELLSGKDEDFR
                     IPESPNGVAQCLITYQNSHRNQDLWHFVTPDHRKSVLWIQLKSGDNKDMTKVVSAMEQ
                     FIAANPAPFGITHHWFGLTYINVIWQEKMVTGMLQSFFGSFFIVLLMMIFMFQSGLWG
                     LLSMIPLTVTIALIYGVIGLVGKDYDMPV"
BASE COUNT          284 a          282 c          276 g          248 t
ORIGIN      
        1 caccggcata tcatagtctt tccctaccag tccgatgaca ccatagatca aggcaatagt
       61 gaccgtgagg ggaatcatag acagaagccc ccacaaacct gactggaaca taaatatcat
      121 catcagaagg acgataaaga aactcccaaa gaacgactga agcattccag tgaccatctt
      181 ctcctgccag atgacattga tgtacgtcag cccaaaccaa tggtgcgtga tcccaaaagg
      241 agctggattg gcagcaatga actgctccat cgccgagaca actttggtca tgtccttgtt
      301 gtccccgctc ttgagctgaa tccacagaac actcttccga tgatccggcg ttacaaagtg
      361 ccagagatcc tggttgcgat gactgttttg gtacgtgata aggcactgcg ccacaccgtt
      421 tgggctttcg gggatccgga aatcctcatc cttgccggac aaaagttcgc gatagaccgt
      481 cttcacgatg tctgagaggg agttggactt tccaacaatg cccgtctcca gcagtgcttc
      541 ctgaagcctt gagatatagg caagcgcttc aggttgtttg aaaacttcat cccgttgttt
      601 ttcgatctcc aggaacaggg aggcctgatc ccaggcatcg tagagttcat cgggagccgt
      661 atccaactgc gaattgacat ccatagaaag ggcatccagc aactggtgct tggcttgcac
      721 acttgccacc agcatggata gcttctctcg cacgatgcgg aagatctcat ggcttccggg
      781 gtaggatgac tccagctccg tgcccttgtc gtacaaacgg tttcccagat cgctgacgta
      841 ttccggaaga tcggagtgtt catccgaagg ctccagagcc agataggcag tgtatgttcc
      901 gccaaaatgt gagtttagaa cgcgatcggc cactcggatc ggatgagaag gcgcaaacca
      961 gcgcgtaggg ttgtcattaa tctgaatctg gctgatgccg tagccggcga caacaacaca
     1021 gagaaccaca aacgcaagaa ccaatttgga gttcttgtag gtgaaacgtc cggtgttgcg
     1081 aagaatggtt
//
LOCUS       DAOQUC010001597         1090 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_199169_length_1090_cov_2.235749, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001597 DAOQUC010000000
VERSION     DAOQUC010001597.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1090
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_199169_length_1090_cov_2.235749"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..704
                     /locus_tag="PKH07_20220"
     CDS             <1..704
                     /locus_tag="PKH07_20220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37330.1"
                     /translation="SSPVQCRFEVHLGSDGSVAQNAWNLWLFPKSDRATMAKSLTVWN
                     YSGAESSETLHGIDCTTLKWDDVKQQWIALDREREIVVNDWSNAVVATDRLDTNITAT
                     LQDGARVLYIPYQDSPMDIPRQDAPFWREMTIWLPTGHPALGDFPHESFTDLQFLDMT
                     QRRPFALGKSRDSVNSLIWGTNARNPGANLYDYVYETSVGKGKMIACCLNLRGIDNVA
                     GHHLLRCLAEYLISL"
BASE COUNT          242 a          302 c          275 g          271 t
ORIGIN      
        1 catcctctcc agtgcagtgc cgttttgagg tgcatctcgg ctctgatggc tctgtggctc
       61 aaaacgcctg gaatctctgg ctgtttccaa aatccgatcg agccacgatg gccaaatccc
      121 tcaccgtatg gaactattcc ggagcggaat cgtccgagac tcttcatgga attgactgca
      181 cgactctgaa gtgggatgac gtcaaacagc aatggattgc ccttgatcgc gaaagagaga
      241 ttgttgtcaa tgactggagc aatgctgttg tcgccacaga cagattggac acgaacatca
      301 cggccactct tcaggatggg gcacgtgtgc tctacatacc atatcaggac tctccaatgg
      361 acattccccg ccaggatgca ccattctggc gcgaaatgac aatctggctt ccgacaggac
      421 atccggcact cggtgatttc cctcatgaga gtttcactga ccttcagttt ttggatatga
      481 cgcaacgccg accctttgct ctgggtaagt ctcgcgattc ggtcaacagc ctgatatggg
      541 gaaccaatgc aaggaatccg ggtgcaaacc tctatgacta tgtgtatgaa acttctgtgg
      601 gaaaaggcaa gatgatcgct tgttgtctga acttgcgtgg tattgacaat gtggcaggac
      661 accatctact cagatgtctg gcggaatatc ttatcagcct ttagccgcgt agacacgacg
      721 ctgtattcgg tgtcgaggtt tccgtccatc ttcgtcacca gattcaaacg cttgtcatcc
      781 gatatgtagt gcggcattca tgccgcggtt ttgtccgtgg ttacgccgta aggcgtgatc
      841 ctctcctgtt cgtagtttcc ccgtcagggg tgatccagat atgccctcac gggcacacta
      901 cgaacctgcc gatacaagac aaccgatggg tcacgaccca tcctacggat cactcgcgca
      961 agcaagcttg cgcactccaa aatcttcttc cccgaagtgc ggtagcttgt gctgccgcgt
     1021 tctgtcttta tgcttcggtt gcgctcaggg caggcctcgg agcaccgcta ccgaagcccc
     1081 gtcactacca
//
LOCUS       DAOQUC010001598         1089 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_199396_length_1089_cov_2.691489, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001598 DAOQUC010000000
VERSION     DAOQUC010001598.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1089
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_199396_length_1089_cov_2.691489"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..759)
                     /locus_tag="PKH07_20225"
     CDS             complement(<1..759)
                     /locus_tag="PKH07_20225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37331.1"
                     /translation="MAKFVTADKNQSSITLRAVIIAFACILGISIAGCVSAFLRYDLI
                     GTGHLPRCALYPILVLMLLNYILRRFTRYDGLKKHELLFIYCTILVMTGIPGQQFATY
                     LYFGLVGPIYYATPQNQYAKLFHKYIPEWLVPSRDPDAPVIKWLFEPPEDLGFMDIPW
                     SLWIRPMAVWLPFCLVVFLVTLAIAALLRKQWSDNERLLFPLARVPMDIVDTGQRKEN
                     NLFRNKIMWVFCLIPVIVYFINGMHEYFPAVPRIN"
BASE COUNT          292 a          247 c          304 g          246 t
ORIGIN      
        1 gttgattctt ggcaccgcgg gaaagtattc atgcatcccg ttgatgaaat agacgatgac
       61 cggaatgagg cagaagaccc acatgatctt gttgcggaag agattgttct ctttgcgctg
      121 tcccgtatcc acaatgtcca tgggaacgcg ggcaagagga aaaaggagtc gctcgttgtc
      181 cgaccattgt tttctcaaca acgccgcgat cgccagtgtg accaggaaca ccaccaggca
      241 gaacgggagc cagaccgcca tcggtcgaat ccagagactc cacgggatgt ccatgaatcc
      301 gagatcctcg ggtggttcga aaagccactt gatgaccggt gcgtcgggat ctcttgaggg
      361 taccagccat tctggaatgt acttgtgaaa aagtttggca tactgatttt ggggtgttgc
      421 gtagtagatg ggtcccacaa gtccaaagta aagataggtg gcgaactgct ggccgggaat
      481 gcctgtcatg accagaatgg tgcagtagat gaagagcagt tcatgtttct tcagaccgtc
      541 gtatcgggtg aaacggcgca gaatgtagtt gagcagcatg agaacgagga tcggataaag
      601 cgcacaacgg ggcagatgac cagttccgat caggtcgtaa cgaagaaagg ccgagacgca
      661 tcctgcgatg gaaattccaa ggatgcaggc aaaggcgatg atcacagccc gcagtgtgat
      721 gctgctttga ttcttgtcag cagtcacaaa ctttgccaca aagactcctt tcgacaaagg
      781 ttcggcacta gactccttgt tcccgaacgg acgactcaat gtcagatcac cagtcaatgc
      841 cctttgagac gctccaagtt ctggagttga tgaactttat gaaacttgaa gcgcggcgtc
      901 aagtcttggc gcagctgtaa ttttgcgtcg acttgtgtgc gggatacagg gatagattcg
      961 agatggtctg aataacttag actaactgga atgcttagcg gtgagatcgg tcaagaggtc
     1021 gaaagtaacc agcaatgcaa tctgagtcac gaacacaaat ctgcacatgc gttcttttca
     1081 taaagccag
//
LOCUS       DAOQUC010001599         1088 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_199660_length_1088_cov_3.529526, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001599 DAOQUC010000000
VERSION     DAOQUC010001599.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1088
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_199660_length_1088_cov_3.529526"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..843)
                     /locus_tag="PKH07_20230"
     CDS             complement(<1..843)
                     /locus_tag="PKH07_20230"
                     /inference="COORDINATES: protein motif:HMM:NF015867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspK"
                     /protein_id="HNT37332.1"
                     /translation="MIVLWVVIILSTLVVSLAYQSKMDIKMMGYQMSEVRARYVARAG
                     LARALVYIREDVLKDNDDFPEDREPINEILSEDEGAIMPYDAYIEAWHNNPDNYDNVE
                     FAGGTLDVKVWDESSRLNLNSSYVLANQDVVKGLLLFLGHDEDTAQEVSAAIIDWIDT
                     DTEPSDGGDQADWGDEQSEDTYYNPDQDPDDILEFGPEYVNKNGPLDSVEELLLVRGV
                     TPLILNGEDANGNGELDDNEDDGDANAPSDNEDGDLQLGLKDFVTVHSNRYVNLNTAP
                     WQVIA"
BASE COUNT          239 a          312 c          262 g          275 t
ORIGIN      
        1 tgcaatgacc tgccacggag ccgtgttgag attgacatac cgattcgagt gcacggtcac
       61 aaaatccttc aaacccaact gcagatctcc gtcttcattg tctgatgggg cattggcatc
      121 gccgtcatct tcgttgtcgt caagttcccc gttcccattg gcatcctcac cattgaggat
      181 caacggggtg acgccacgaa ccaagagcag ttcctcaaca ctgtccagag gaccgttctt
      241 gttcacgtac tcaggaccaa actccagaat gtcatccgga tcctgatcgg gattgtagta
      301 ggtgtcttcc gactgctcat cgccccaatc ggcttgatcc cccccgtcgc tgggctcggt
      361 gtctgtgtcg atccagtcta tgatcgctgc agagacctct tgcgctgtat cctcatcatg
      421 tcccaaaaag agaagtaatc ccttgaccac gtcctggttg gcaagcacat aggatgagtt
      481 caggttgagc cgactggatt catcccacac cttcacatcg agggtgcctc ctgcgaactc
      541 gacattgtcg tagttgtctg gattgttgtg ccatgcctct atgtaggcgt catagggcat
      601 gatcgcccct tcatcctctg aaaggatctc gttgattggt tctcggtctt cgggaaagtc
      661 atcgttgtcc ttcagaacat cctctcggat atagaccagc gcccgtgcca ggcctgcgcg
      721 tgccacatac ctcgccctga cttccgacat ctgatagccc atcatcttaa tgtccatctt
      781 tgactgatag gccaaagaaa caactagcgt cgaaaggatg atgaccaccc aaagaacaat
      841 catcagggca actccacgct gactttcccg acatactccg ccaccgtggc aaggaagagc
      901 attcttccgg ctgtcacagg gtgtgcggtc gtcagatgtc agtggccact gttgttttct
      961 gatcatgtat ccaagcctca tcgcatatta ccggcttgat ccagtgcatc ccgataggga
     1021 gtctcgttca gagtgctcga cgggatttct gtcgtggtca acagcaagga gttgtacgag
     1081 agcatgaa
//
LOCUS       DAOQUC010001600         1087 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_200004_length_1087_cov_2.776163, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001600 DAOQUC010000000
VERSION     DAOQUC010001600.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1087
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_200004_length_1087_cov_2.776163"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="PKH07_20235"
     CDS             <1..240
                     /locus_tag="PKH07_20235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37333.1"
                     /translation="SPAWRAKSRTGICLLRWNLLMEQKHCNREECVCYLEDDDFLDEA
                     NHPVSNCTFDKFVKFDPDAPCPYYRLDWKKASRKK"
     gene            256..>1087
                     /locus_tag="PKH07_20240"
     CDS             256..>1087
                     /locus_tag="PKH07_20240"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNT37334.1"
                     /translation="MSHTGRRRIRAFTLLEILVVVSLTAIIALITIPTLRSHLQKAKV
                     NKTRLLISTLENSIWSYYADTGQFPPSGSDFLQECLLHGLVNTDKDETRIEAGRTSNP
                     HWNGPYITLDNDQLLKPGDLEKKMPRFLDGTPMISSSARSGSSFIVDSWGTPFLYVNS
                     ETMLWAAMNGNGQGYQGDGNVGPYYPAWHENEDGQLSISPPRNRDEVKDYHNYSAGKT
                     AAGGSKAFQLYSVGADGKTRSGDGGPGTLSIHDGRDNDGDGLIDEKDNDRSTRNGTPP
                     E"
BASE COUNT          271 a          284 c          293 g          239 t
ORIGIN      
        1 agccccgcct ggcgcgccaa gtcccgaact gggatttgcc tgttgaggtg gaatttgctt
       61 atggagcaga aacattgcaa ccgagaagaa tgcgtttgct atcttgaaga tgatgacttt
      121 cttgatgagg cgaaccatcc tgtcagcaat tgtacctttg acaagttcgt gaaattcgac
      181 cctgatgctc cctgccctta ctaccgtctg gactggaaga aagccagcag gaaaaagtag
      241 gaaggagatg catctatgag ccatacagga aggcgtcgaa ttcgggcctt cacgctcctg
      301 gagattctgg ttgttgtttc cctgacagcg atcattgctt tgatcaccat tccaaccctg
      361 cgttcccatc tgcagaaggc gaaggtcaac aaaaccagac tgcttatcag cacactggaa
      421 aactcgatct ggagttacta tgccgatacc gggcagtttc cccccagtgg atcggatttc
      481 cttcaggagt gtcttttgca tggcctggtc aacacggata aggacgaaac acggattgag
      541 gctggtcgca cctccaatcc acactggaat ggcccctaca tcactctgga caacgatcag
      601 ctgctcaagc cgggagactt ggaaaagaag atgccaagat tcctggatgg aactccgatg
      661 atttcctcga gtgcgcgttc gggatcttca ttcatcgtag actcctgggg caccccgttt
      721 ctctacgtga acagtgagac catgctgtgg gctgcgatga acggaaatgg acagggctac
      781 cagggcgatg gcaacgtggg tccttactac cccgcgtggc atgagaatga agatggccaa
      841 ctgagcatct cccctccaag aaatcgcgat gaggtcaagg actatcataa ctactctgcc
      901 ggcaaaaccg ccgctggtgg atcgaaggct tttcagcttt actcggttgg cgcagatgga
      961 aagacccggt cgggcgacgg cgggcctgga actctttcga ttcacgatgg cagagataat
     1021 gatggggacg gcctcattga tgaaaaggac aacgaccgct ccacccggaa tggaacccca
     1081 cccgaag
//
LOCUS       DAOQUC010001601         1085 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_200684_length_1085_cov_2.652427, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001601 DAOQUC010000000
VERSION     DAOQUC010001601.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1085
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_200684_length_1085_cov_2.652427"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1085
                     /locus_tag="PKH07_20245"
     CDS             <1..>1085
                     /locus_tag="PKH07_20245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HNT37335.1"
                     /translation="AGAQRAIRKLKGEGLLTEPVRVIVAQGDFKLSETLVFTPEDSGT
                     KECPIIFEAAPGATPVFSGGRRIKGFKKDRKGIWKTTIPEVKRGEWYFEQLFVNGRRA
                     LRARTPNQSAFRMMQPLGKGTDPLTGEETDLSHRGFIGSATDLKVLKNFEDAWLVVYH
                     AWEISHSRIASLDSKSGTLITTAPACWEMFKWDKQPRYHLENFREALDEPGEWFLDRK
                     GTLYYIPLPGEDPKTAEVIAPVLERFVEIQGVASEGRFVENLTFRGLSFVHGEYRLPE
                     SGYQCPQAAFSLSAAVLADGARDIHFEDCEIAHIGHYAVWFRNGCRDCSIEKCLIHDM
                     ASGGIRIGEPQIRESEKEHTSHITLDN"
BASE COUNT          261 a          284 c          313 g          227 t
ORIGIN      
        1 tgccggcgca cagagggcga tccgaaagct caaaggcgag ggactgctca cggaacctgt
       61 tcgagtcatc gtggcacagg gggacttcaa gctgagcgag actcttgtgt tcacacccga
      121 agacagcggg acaaaggagt gtccgattat ctttgaagcc gcaccaggag caacgccggt
      181 cttttccggc ggtcgtcgaa tcaaaggttt caagaaagac cgaaagggaa tctggaagac
      241 caccattcct gaagtcaaaa gaggggaatg gtatttcgag caactgttcg tcaacggccg
      301 tcgagctcta cgtgcgagaa cacccaatca gtcggcgttt cgcatgatgc aaccgttggg
      361 taaaggcacg gatcctctga caggggaaga gaccgacctt tcacatcgag gattcattgg
      421 cagcgcaacc gacttgaaag tactgaagaa ctttgaagat gcctggttgg ttgtgtatca
      481 cgcctgggag atctcccaca gccgcatcgc ttcgctggac agcaaatccg gaaccctcat
      541 cacgacagcc cccgcctgct gggagatgtt caaatgggac aaacaacccc gctatcatct
      601 ggagaacttt cgggaagcgc tggacgaacc tggggagtgg tttctggatc gcaagggcac
      661 gctgtactat attccactgc ccggtgagga tccgaagact gcggaagtga ttgctccagt
      721 gttggagcgc tttgtcgaga ttcaaggcgt tgcttcggaa gggcgatttg tcgagaacct
      781 taccttcaga ggactctcct ttgtccacgg agagtaccgc ttacccgaaa gtggctacca
      841 atgtccgcag gctgcctttt cgctgtcggc agccgtgctt gccgatgggg cacgtgacat
      901 tcactttgag gactgtgaga ttgcacacat cgggcactat gcggtctggt ttcgcaacgg
      961 gtgccgtgac tgttcgatcg aaaagtgcct catccacgac atggcatccg gcggtattcg
     1021 cattggagag cctcagattc gggagtccga aaaggaacac accagtcaca tcaccttgga
     1081 caaca
//
LOCUS       DAOQUC010001602         1084 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_200943_length_1084_cov_3.447036, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001602 DAOQUC010000000
VERSION     DAOQUC010001602.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1084
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_200943_length_1084_cov_3.447036"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..380)
                     /locus_tag="PKH07_20250"
     CDS             complement(<1..380)
                     /locus_tag="PKH07_20250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020574272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HNT37336.1"
                     /translation="MNIPRPEYPRPQFVRSEWLCLNGEWEFEIDAGDSGLARGLLNKP
                     LKDRIVVPFCPESPLSGIGNEDFLNVVWYRRTVRIPKEWSGKDVLLHFQAVDYDTTIW
                     VNGTEVNRHRGGFTPITCDLSRVA"
     gene            complement(463..>1084)
                     /locus_tag="PKH07_20255"
     CDS             complement(463..>1084)
                     /locus_tag="PKH07_20255"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018195327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNT37337.1"
                     /translation="TTTVPSLSQEICAAALAKDAFALDAPVSGGDVGARNATLSIMVG
                     GDQQTFQAVLPLLERLGKSIVYQGQSGAGQHTKMCNQIIITGTMIGVCESLIYGTKAG
                     LDLETVLKSISSGAAGCWTLTNLAPRILKRDFNPGFFVEHFIKDMGIALDEARKMNLS
                     LPGLALVHQLYLALQAQGHGRCGTQALALALEQLSNVKIEHKPVAL"
BASE COUNT          256 a          316 c          273 g          239 t
ORIGIN      
        1 tgcgcaacgc gactcaaatc gcaggtgatc ggagtgaatc cgccccgatg tcggttgact
       61 tcggttccat tgacccagat cgttgtatcg tagtcaacag cctgaaagtg caggagaaca
      121 tctttgccag accattcctt gggaatgcga acagtgcggc gataccagac gacgttgagg
      181 aaatcctcgt tgccgatacc cgaaagaggg gattccggac agaacggaac aacgatgcga
      241 tccttcaagg gcttgttgag cagtccgcga gccagaccac tgtctccagc atcaatttca
      301 aattcccact caccattcaa acaaagccac tccgaacgaa caaactgagg tctgggatac
      361 tcgggacgag ggatgttcat gacagctgat tcctccaata caactccgtg ttctctgccc
      421 atcggcgaga taccaatggg atcatctcta cccattgcgc cttcagagcg caaccggttt
      481 gtgctcaatc ttcacgttgg acaattgttc cagggctagt gccaatgcct gtgttccaca
      541 gcgtccatga ccctgagcct gaagtgccag atacaactga tgtacaagcg ccaacccagg
      601 aaggctcagg ttcatcttcc gagcttcgtc gagcgcgatg cccatatcct tgatgaagtg
      661 ctcaacgaaa aagcctgggt tgaaatccct tttcaggatt cgcggggcaa ggtttgtcag
      721 tgtccagcac cccgcagcgc cggaactgat agacttcaat accgtttcca gatcaagtcc
      781 cgccttcgtt ccatagatca ggctctcaca caccccgatc atcgttcctg tgatgatgat
      841 ctggttgcac atcttggtgt gttgccccgc acccgactgc ccttgataga caatgctctt
      901 gccgagtcgt tccagcaggg gcaacactgc ctgaaaagtc tgttggtcac caccgaccat
      961 gatggaaagc gtggcgttgc gagcgccaac gtcaccaccg gataccgggg cgtccagcgc
     1021 gaacgcatcc tttgccaatg ctgcggcaca aatctcctgc gacaggcttg gaaccgtggt
     1081 ggtc
//
LOCUS       DAOQUC010001603         1083 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_201272_length_1083_cov_3.567121, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001603 DAOQUC010000000
VERSION     DAOQUC010001603.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1083)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1083)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1083
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_201272_length_1083_cov_3.567121"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            143..391
                     /locus_tag="PKH07_20260"
     CDS             143..391
                     /locus_tag="PKH07_20260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37338.1"
                     /translation="MRNLDEYDVGWLDLEVRSVPVRAYSEKRLTASLQTESDWNSVST
                     NNSWLKTDGIGIIFSHRRFRLPDRPHPPVELDDEDVQC"
     gene            477..>1083
                     /locus_tag="PKH07_20265"
     CDS             477..>1083
                     /locus_tag="PKH07_20265"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HNT37339.1"
                     /translation="MDRLGNVATLTDQTGSAVHTFEQDAYGNAIESIVTGIWQTSYGG
                     LHLNGREHDSDTSLYFFKHRAYDPTIGIFVSKSPLAPFREHPYAYVHSSPTNFVDPYG
                     LLADIPRIGPKPAPTPEITPMPTPTPGPPRPPYPDISYGNWCGPEWPPKEAKPRPDPR
                     DALDKCCMEHDLCYEREGVVWNDPSTWLPGKFACDTDLCRCL"
BASE COUNT          280 a          279 c          256 g          268 t
ORIGIN      
        1 cccgaattct gagatatcaa atttgagatc tcaaatcgga atacctgagt gctcatacct
       61 catcgctcat ttccgattcg ggttgaacac tttgggcgaa atacggtata tttgagaaga
      121 cgttgcaaga cggggtttgt agatgagaaa tcttgatgaa tatgatgttg gctggctgga
      181 tttggaggtt cgtagtgtgc ccgtaagggc atattccgag aaacgcctta cggcgtcact
      241 acaaaccgaa tccgactgga actcagtcag cacgaacaac tcctggctga aaaccgacgg
      301 catcgggatc atcttcagcc accggcgatt ccgacttcca gatagacctc atccgcctgt
      361 cgaactcgat gacgaagatg ttcaatgtta aaactcctgg gaatgccagc aagatatcaa
      421 ataggcattt gttcgaagaa tatggcactt cccgtcactc aatggtatgg ttgcatatgg
      481 acagactcgg aaacgttgcg actctaacgg atcaaacagg ttctgccgtt catacatttg
      541 agcaggacgc atacggcaat gctattgaaa gtatcgtaac tggaatatgg cagacctcct
      601 acggtggatt acatttgaac ggacgagagc atgattccga cacgtccctc tatttcttca
      661 aacacagagc atacgatcca acgattggta tctttgtctc gaagtcacca ctggctccat
      721 ttcgggagca tccttacgcc tatgttcatt cttctccgac gaatttcgtt gatccgtatg
      781 gtcttctggc ggatattcca agaatcgggc cgaaacctgc cccaacgcct gaaatcacac
      841 caatgccaac tcctactcca ggtccacctc ggcctcccta tcccgacata agttatggca
      901 actggtgcgg cccggagtgg cctcccaagg aagcgaaacc cagacccgac cctcgcgatg
      961 ctcttgacaa atgttgcatg gaacacgatc tgtgctatga acgggaaggc gtcgtctgga
     1021 acgatccttc tacttggcta cctggaaagt ttgcgtgtga cacggatctc tgcagatgct
     1081 tgc
//
LOCUS       DAOQUC010001604         1082 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_201611_length_1082_cov_2.630964, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001604 DAOQUC010000000
VERSION     DAOQUC010001604.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1082
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_201611_length_1082_cov_2.630964"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1082
                     /locus_tag="PKH07_20270"
     CDS             <1..>1082
                     /locus_tag="PKH07_20270"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNT37340.1"
                     /translation="EDLIGAMVGRSVSFERDPDCVPCDGQRLECRETGAEIATLQVKE
                     GEIFGIYGLVGSGQPELCQALFGLRDTPRMSISLNAKEIGNLVTSDRVRAGLGYVPAD
                     RLEQGLFYQMTVGENMSLASLSGLTRLGFINESQEQRINDDLIQKLRVKTQGIEQRVN
                     QLSGGNQQKVLLARWLESQPQLLILEEPTQGVDVGAKGEIHKIITSLAKSGVSILLIS
                     SEIPELMALSHRIGIMRDGKLVAELDATSATEEEILREALPNSVSQMKSDSAMQEHRE
                     RHPLSRLGRKIFEQRELSIALFIGLIIVVFGVTTPGFATWRNLSDVLVNNSIVLIGAL
                     GIMFVIISGGIDISVGAIMGLAAVAT"
BASE COUNT          244 a          241 c          333 g          264 t
ORIGIN      
        1 tgaagatctg attggtgcca tggtcgggcg ctcggtttct tttgaacgcg atccggattg
       61 tgttccgtgt gatggtcagc gactcgaatg tcgggagacg ggagctgaga tagccacgct
      121 ccaggtcaaa gaaggagaga tttttggcat ttacggtctg gtgggttctg gtcaacccga
      181 actctgccag gcattgtttg gccttcgcga tacgcccagg atgagtattt cgttgaacgc
      241 gaaagagatc ggaaatctgg tgacatctga cagggtgaga gcgggtcttg gctatgttcc
      301 cgcagaccgc ctggagcagg gacttttcta ccagatgact gtcggggaga acatgagcct
      361 tgcttcgctg agcggtttga ctcgattggg gttcatcaac gagagtcagg aacaacggat
      421 caacgacgat ctgattcaga aattgcgagt caagacgcaa gggattgagc aaagggtcaa
      481 ccaactgtcc ggtgggaacc agcagaaggt cttactggct cggtggctgg agagtcaacc
      541 gcagttgttg attcttgagg aaccaactca gggtgtggat gtgggtgcca aaggcgagat
      601 tcacaagatc atcaccagcc ttgccaagtc gggtgtctca atcctgctga tcagttccga
      661 gattcctgaa ctcatggcac tttctcatcg aatcggcatc atgcgggacg gaaaactggt
      721 tgccgaactt gacgcgacca gcgcgacgga agaagagatt cttcgagagg ctctgccaaa
      781 ttcggtgagc cagatgaaaa gcgattcggc gatgcaggaa cacagagagc ggcatcctct
      841 gtcacgtttg ggtcggaaga ttttcgaaca gcgggagctg agcattgccc tgtttatcgg
      901 tctgatcatc gtcgttttcg gtgtcaccac tcccggattt gcgacttggc gcaatctgag
      961 tgatgtgctt gtcaacaact ccattgtgct cattggggca ttggggatca tgttcgtgat
     1021 tatctcagga gggatagata tctcggtagg tgcgatcatg ggacttgcgg ctgttgctac
     1081 gg
//
LOCUS       DAOQUC010001605         1082 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_201616_length_1082_cov_2.547225, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001605 DAOQUC010000000
VERSION     DAOQUC010001605.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1082
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_201616_length_1082_cov_2.547225"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..606)
                     /locus_tag="PKH07_20275"
     CDS             complement(<1..606)
                     /locus_tag="PKH07_20275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HNT37341.1"
                     /translation="MKDFRATSFTVCCALVMMGFLSVTCHSTEPVGCMGGAAEPAVAL
                     LQTHPTLDQLQKAFANAAQEFSVPVEIVKAVAYKESAWVHSGPTVDYGYGIMHLVDNE
                     YCQTLREAAALVGLDPETLKRDPIANIRGGVALIAKYAKETIGKPKTLEDYWDVLKKF
                     SGLLGDDVQAIQAEEYFALIHDGFSAQNTLGMQIKVAGTPVD"
     gene            complement(852..>1082)
                     /locus_tag="PKH07_20280"
     CDS             complement(852..>1082)
                     /locus_tag="PKH07_20280"
                     /inference="COORDINATES: protein motif:HMM:NF027688.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcineurin-like phosphoesterase C-terminal
                     domain-containing protein"
                     /protein_id="HNT37342.1"
                     /translation="RPMQQFTGHDPFFQQLKELEATHSLPGKKLPDPARTEHLWKASL
                     PESLTTGAHLIRVRTVDMFGHQYQASRSVFVR"
BASE COUNT          270 a          302 c          280 g          230 t
ORIGIN      
        1 gtccacaggg gttcctgcca ctttgatttg cattcccaaa gtgttttgtg ccgaaaagcc
       61 atcatggatc agggcaaagt actcctcggc ttgaattgcc tgaacgtcgt ctcccaaaag
      121 accgctgaac ttcttgagca catcccaata gtcttccagc gtcttgggtt ttccaatggt
      181 ctccttggcg tatttggcaa tcaaggcgac accgccacgg atattggcaa tcggatccct
      241 tttcagtgtt tctggatcga ggccgacaag cgctgcggct tctctcagag tctggcagta
      301 ctcgttgtcc accagatgca tgatgccgta gccatagtcc acagtcggcc cactatgaac
      361 ccaggcggat tccttgtaag cgacggcttt gacgatttcc accggaacgc tgaactcctg
      421 agctgcgttg gcaaaggctt tctgcaactg atccagagtc ggatgagtct gcaagagcgc
      481 aacggcaggc tcggcagccc cacccataca tccgaccggt tccgtcgaat ggcacgtcac
      541 tgagagaaag cccatcatca cgagagcaca acaaacggtg aaactggtgg cacggaagtc
      601 cttcatagca gatctccaac tcatacaaga aatggctctg gactggccgt tgcgaggcaa
      661 tatccaaagc agtcggggca aaacgccaag ccagcgggat ggacacactt tcgcaaccca
      721 ggacacagac aaaacagatc ggtcaaacca ctcatttcaa aaagcctgtc catctctcga
      781 caaaacagat cctgtccaca ccaatgtaac gtagcacagc atcgcacaag cgtcaaaaga
      841 agcgccactc ctcagcggac gaaaacggat cttgacgcct gatactgatg accgaacatg
      901 tccactgttc gcacacggat cagatgtgcg ccagttgtga gggattcggg caatgatgcc
      961 ttccagaggt gctcggttcg tgcgggatcg ggcagtttct tgcccgggag agaatgggtg
     1021 gcctccagtt ccttgagttg ctggaaaaag ggatcatggc ctgtgaactg ctgcatggga
     1081 cg
//
LOCUS       DAOQUC010001606         1081 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_201834_length_1081_cov_3.678363, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001606 DAOQUC010000000
VERSION     DAOQUC010001606.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1081
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_201834_length_1081_cov_3.678363"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..410)
                     /locus_tag="PKH07_20285"
     CDS             complement(<1..410)
                     /locus_tag="PKH07_20285"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNT37343.1"
                     /translation="MNLAIDYDGTIADTNSEKIRWIRENLGREDVPHWLCNHTECAPI
                     IGEADYRAMGSYVYERESTLAATEVPGALDALRTLSESGHRLLLLTARPVRRQEYSME
                     WLRAHGVEHLFADALSSHDSTKSAVCEQIGAQML"
     gene            complement(674..994)
                     /gene="ilvN"
                     /locus_tag="PKH07_20290"
     CDS             complement(674..994)
                     /gene="ilvN"
                     /locus_tag="PKH07_20290"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006323570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HNT37344.1"
                     /translation="MPALETTNQERHNTPRAILEVTVRNHPGVMSHVCGLFSRRAYNM
                     EGILCMPVGDGTTSRMWLLVKDDVRLEQMVKQIHKLEDVLSIRRHGADHAVFVGLEDF
                     FQEQ"
BASE COUNT          243 a          302 c          271 g          265 t
ORIGIN      
        1 ataagcattt gggcaccgat ctgttcgcag actgctgatt tggtggagtc gtgagaactg
       61 agagcatcgg caaacagatg ctccacccca tgggcgcgga gccattccat cgaatactct
      121 tgtcttctga caggtctggc ggtcaagagc aagagtctgt gcccggattc cgaaagggtg
      181 cgcagtgcgt ccaatgcacc cggcacttca gtggccgcga gcgtggactc tcgttcatat
      241 acgtaacttc ccatagccct atagtcagct tcgccgataa ttggtgcaca ttcggtatgg
      301 ttgcacagcc aatgaggcac atcctcgcgc cccagattct ccctaatcca tcgaattttt
      361 tcggagttgg tgtccgcgat ggttccatca tagtcaattg ccagattcac tctcatccac
      421 tcctaccatg tgttctgcgc ctgactgtca gcagcagcca ttttccatca cgatagatca
      481 tgacttgatg atagtattcc atgattcgca cagaccgtcc atgtcgaatg tatgggcaaa
      541 ccttccggtc tccaactcag ggcaaaaaac tctggaacag gttagcgcct acaagcgtgt
      601 ggactacgaa caatctcaca gatacttgct cgttgacatc tctgccccag tcaggaagca
      661 ggaacgctct cgttcactgt tcttgaaaga agtcttccag cccgacgaag acggcatggt
      721 cagcaccatg gcgacgaatg gaaagcacat cctcaagttt gtgaatctgc ttgaccatct
      781 gctcaagacg aacgtcgtcc ttgaccagca gccacatccg actcgttgtt ccgtctccca
      841 cgggcataca gagtatgcct tccatgttgt acgcgcgtcg cgagaagaga ccgcacacat
      901 gagacatgac tcccgggtga ttgcgcaccg tgacttccag aatcgcccga ggtgtgttgt
      961 gtcgttcttg attcgttgtt tcaagcgcag gcatttgcat ggcgttcacc tccgatagta
     1021 tctctgttgg cagctccagg gggcaccatg ggataaacgt tgtcgttggg ctccaggggc
     1081 a
//
LOCUS       DAOQUC010001607         1081 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_201852_length_1081_cov_3.201754, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001607 DAOQUC010000000
VERSION     DAOQUC010001607.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1081
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_201852_length_1081_cov_3.201754"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="PKH07_20295"
     CDS             <1..291
                     /locus_tag="PKH07_20295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37345.1"
                     /translation="DNDLEQYDDILIEEWAIRFDQAKDSLPVDPSSDDQIASGKKVFQ
                     WVEAEAHIPIRPSCQESFITRGTYQMLANRLRVGWHPEFQSRLVAQSAEDNL"
     gene            288..782
                     /locus_tag="PKH07_20300"
     CDS             288..782
                     /locus_tag="PKH07_20300"
                     /inference="COORDINATES: protein motif:HMM:NF039805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6521 family protein"
                     /protein_id="HNT37346.1"
                     /translation="MKEWANRPPEIAALLNPAFCGFLMSTGLDAYTQNVNEGAPYAFP
                     FVMLPLVLHKPTRQAFPRSSRTAFSTWITNADTAIAKVGFAERAKNMAPYVKEALAFA
                     MQHNSIYVTESGRLKTSNPAPKSFPNATQEVNECARAALMCGKWFSMVGDFKTAMALL
                     GVKP"
     gene            779..>1081
                     /locus_tag="PKH07_20305"
     CDS             779..>1081
                     /locus_tag="PKH07_20305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3732 domain-containing protein"
                     /protein_id="HNT37347.1"
                     /translation="MKFQILNILVYGANGQIRSIELKPDAVNVVTGQSKTGKSALIHI
                     VDYCLGRRECNVPAGVIRKHVKWYAVKLQTSSGEVFIARRNPEPGKESSEDIYIERG"
BASE COUNT          268 a          281 c          280 g          252 t
ORIGIN      
        1 gataatgatc tggagcaata tgacgacatc cttatcgaag aatgggcgat ccgattcgac
       61 caagcaaagg attccctgcc tgtagatcca tcatctgatg atcaaatagc ttcagggaaa
      121 aaggtttttc agtgggtgga agcggaggcc cacatcccaa ttcgcccctc atgtcaggaa
      181 tctttcataa ccagaggcac gtatcagatg ctcgcgaacc gcctacgggt cggctggcat
      241 cctgagttcc agtcgcgcct ggttgctcaa tctgcggagg ataatctatg aaggaatggg
      301 cgaacagacc tccagagatt gcagcgcttt tgaacccggc attttgcggg tttctgatgt
      361 caaccggtct ggatgcatac acacagaacg tcaacgaggg tgcgccgtat gcttttccct
      421 tcgtgatgtt gcccctggtc ctgcacaagc cgacaagaca ggcattccca cggtcctctc
      481 gcacggcatt ttcaacttgg atcaccaatg cagataccgc aatagccaaa gtaggctttg
      541 cagaacgcgc aaagaacatg gctccctacg tcaaggaagc actggccttt gccatgcagc
      601 acaacagtat ctatgtcacg gagtccgggc ggctcaagac ctcgaatcca gctccgaagt
      661 catttcccaa tgcgacccag gaggtcaatg agtgtgcccg tgccgctttg atgtgcggca
      721 agtggttttc catggtcggt gacttcaaga ctgcaatggc gctactggga gtaaagccat
      781 gaaatttcag attcttaata ttcttgtcta cggggcgaac ggccaaattc gctccattga
      841 gctcaagcca gatgctgtca atgtagtaac cggacaatcg aagacgggca aatctgctct
      901 tattcacatc gttgattact gccttggtcg gagggaatgc aacgttcctg ctggggtcat
      961 ccgtaagcat gtcaagtggt acgcagtgaa gctccaaacc tcgtcgggag aggtctttat
     1021 tgctcggcgc aatcctgagc ctggcaaaga atcctctgaa gacatctaca tcgagcgtgg
     1081 a
//
LOCUS       DAOQUC010001608         1081 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_201882_length_1081_cov_2.824561, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001608 DAOQUC010000000
VERSION     DAOQUC010001608.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1081
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_201882_length_1081_cov_2.824561"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1081)
                     /locus_tag="PKH07_20310"
     CDS             complement(<1..>1081)
                     /locus_tag="PKH07_20310"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HNT37348.1"
                     /translation="ANQGSREQRLHDFISAVRTIYASTLSEEALNYRARREMLEQDEQ
                     MALLVQRVSGVVQGTLFFPHVAGVGFSYNPYVWNREIDPKAGVLRLVYGLGTRAVDRT
                     DDDYTRLVALNAPDRRPVAEIEEVYRYAQKRVDVLDLQANQLVSERFDDVVRQSQDPR
                     VDLVATKDETLAEQSSGKTGTEIFPWILTFDPLLQETDYVATMKRMLEALEQAYGCHV
                     DVEFTTNFFKQNQYRINLVQCRPLQVKGEGVVSRPPDSIPAEDLILEAAGAVIGQSRQ
                     TLIERIIQVVPAVYGQLPIGDRYSVARLIGRIMHLRDRVVPRNIMLIGPGRWGTSTPF
                     LGVPVKFGEINNVAVLVEVVEMRDNL"
BASE COUNT          259 a          324 c          263 g          235 t
ORIGIN      
        1 caggttgtca cgcatctcga cgacttcgac aaggacggca acattgttga tctcgccaaa
       61 cttgaccggc actcccagaa agggtgtgga tgtgccccaa cgccctggac caatcaacat
      121 gatattcctg ggcacgactc gatctctgag atgcatgatt cggccaatca ggcgcgcaac
      181 cgagtaacga tctccaatgg gtaattgacc atagactgca ggaacaacct ggatgatcct
      241 ctcgatcaaa gtttgccgac tctgaccgat caccgcccct gccgcttcga ggatcaaatc
      301 ctctgcggga atcgagtcag gcggtcggct gacgactccc tcccccttga cctggagagg
      361 acgacactgc accaggttga tccgatattg gttctgcttg aagaagttgg ttgtgaactc
      421 aacatccaca tggcatccat aggcctgctc cagtgcctca agcatgcgct tcatggtggc
      481 aacataatcg gtttcctgaa gcaggggatc gaaagtcaat atccatggaa agatttctgt
      541 accagttttg cctgaactct gctccgccag tgtttcatcc ttggtcgcca ccaaatccac
      601 tctgggatcc tggctctgcc tgacgacatc atcaaaacgc tccgagacaa gttgatttgc
      661 ctgcaagtcc agaacatcta cgcgcttctg agcgtagcga tagacttcct cgatctcggc
      721 aaccggccgg cgatccggag cattcagagc aaccagccga gtgtaatcat catcagtgcg
      781 atccacggcg cgagttccca gcccgtacac caggcgaagc acacccgcct ttggatcaat
      841 ctcccgattc cagacataag ggttgtatga gaatccgaca cctgccacat ggggaaaaaa
      901 caatgtgccc tgtaccacac cggagaccct ctgcaccagg agcgccatct gttcgtcctg
      961 ttcgagcatc tcgcgccgcg cccgatagtt caaagcctct tcactgagtg tactggcata
     1021 gatggttcgc accgcagaga tgaaatcatg cagacgctgt tcacgcgatc cctgattggc
     1081 a
//
LOCUS       DAOQUC010001609         1080 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_202379_length_1080_cov_1.471220, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001609 DAOQUC010000000
VERSION     DAOQUC010001609.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1080
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_202379_length_1080_cov_1.471220"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /locus_tag="PKH07_20315"
     CDS             complement(<1..269)
                     /locus_tag="PKH07_20315"
                     /inference="COORDINATES: protein motif:HMM:NF013698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNT37349.1"
                     /translation="MTTSTENYVGIDVAKDKLDVAMLGQRATSEVSNTKKGITALVKK
                     MRQLDPRLIVVEATGGYEEVLVLSLFEAGLPVALVSPQRVRQYAKA"
     gene            complement(302..>1080)
                     /locus_tag="PKH07_20320"
     CDS             complement(302..>1080)
                     /locus_tag="PKH07_20320"
                     /inference="COORDINATES: protein motif:HMM:NF012364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase domain-containing protein"
                     /protein_id="HNT37350.1"
                     /translation="AELFKARASALKWLLVVCFGYLGYKNARFGRIESHEAVTAISRE
                     LMLQAKEVAEDMGFTVLHMYVDCLFVQQEGFHRPSDFTKLMNAIEEKTSIPIALEGVF
                     KWVAFLASKRDARVPVPNQYFGAFQDGTLKYRGIELRRRDTTLWVRKIQLKALEVLAQ
                     ADSPQEFADYVPEVMKFVEHAKQELRSGRVPIDELIIHQKLSRGVEAYKTPSPASKIE
                     KDTADRCPLVPQRTPCSTGRRVLCAVELEQLAGLEDRLLA"
BASE COUNT          246 a          321 c          234 g          279 t
ORIGIN      
        1 gcttttgcgt attggcgtac tcgttgcggg ctgaccaagg caacaggtaa gcctgcctca
       61 aacagactca acaccaacac ttcttcatac ccgcctgtgg cttccaccac gatcaacctc
      121 ggatccaact gacgcatttt cttcaccaag gctgtgattc ccttttttgt gttggacact
      181 tcgctggtcg ctctctgtcc caacatggcc acatccaact tatctttcgc cacatcgatc
      241 ccaacatagt tttccgtaga ggttgtcatt tgctactcct gtccgtataa tagaaacgag
      301 ctcacgctag taatcggtct tcgagacctg ccaactgttc gagctcaacc gcacacagga
      361 cacggcgacc cgtgctgcac ggcgttctct gtggaacgag tggacatcga tctgccgtgt
      421 ccttctctat cttactagct ggagatgggg tcttatatgc ttcaactccc cggcttaact
      481 tctggtggat gatcaattca tctattggaa ctcgccctga tcgaagttcc tgttttgcat
      541 gctccacgaa cttcatcacc tctgggacat aatctgcaaa ttcctgaggt gagtctgcct
      601 gtgccagaac ctccaacgcc ttcaattgaa tctttctcac ccacagggtc gtatcccttc
      661 gacgcaactc gatgccgcga tatttgagcg tgccatcctg gaaggcgccg aagtattggt
      721 tgggaacagg cacacgtgca tcccgcttcg aagccaggaa agcaacccac ttgaagactc
      781 cttccaatgc aatgggaata ctcgttttct cctcgatggc attcatgagt tttgtaaagt
      841 cggacggtct gtgaaagcct tcctgttgaa caaacagaca atcgacatac atatgcagaa
      901 ccgtaaagcc catatcttcc gccacctcct tggcttgcag catcaattcg cggctgatgg
      961 cggtcacggc ttcatgcgat tcgatcctgc caaagcgggc gttcttatat cccaaatagc
     1021 caaaacacac caccaacagc catttgagcg ccgaggcccg cgctttgaag agttctgccc
//
LOCUS       DAOQUC010001610         1075 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_203698_length_1075_cov_4.242157, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001610 DAOQUC010000000
VERSION     DAOQUC010001610.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1075
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_203698_length_1075_cov_4.242157"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1051)
                     /locus_tag="PKH07_20325"
     CDS             complement(<1..1051)
                     /locus_tag="PKH07_20325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37351.1"
                     /translation="MPLALLSVCAQRFRVLSHWLPAYGSLVVSALGTAAACLYFRMNR
                     LKPAIILLLFMLACYWILWLDDRRLLRIPTRKSLIAFTRSHVVPLFLLGSLVVAFLVR
                     RALPVALPEWVFATLLIPIFLLFERSYSPDLPSVTSNAAADNVGGRPWRPWISWCLVL
                     LSCLCAALCSYLGWKGSIELEFRFLGLVAPVLWFAGVLTIARVSRKPILWSGILLSVL
                     LQLGAASMWWSKKHPNAALLPFFLSWIPALVATVWIDRLRGLKPSKPIAPRWRIWEVV
                     ALVLILGVASYFRYWRLLEIPYGIWFDEAREAMDGMAPFEGKPYSPLAYYAWSGTLSF
                     NAYLLALCYKVFGFGL"
BASE COUNT          312 a          306 c          288 g          169 t
ORIGIN      
        1 ccagaccaaa tccgaatacc ttgtagcaca acgccagcag atatgcgttg aacgagagcg
       61 ttccactcca ggcatagtat gcaagtgggc tgtacggttt gccctcaaag ggagccatcc
      121 cgtccatcgc ctcacgcgct tcatcgaacc agattccata gggaatctcc agaagtctcc
      181 aataacgaaa gtacgaagcc acaccgagga tgagcacaag cgcgacaact tcccaaatcc
      241 gccaacgagg cgcaatgggt ttggaaggct tcagcccgcg caggcgatcg atccagaccg
      301 ttgccaccag ggcgggaatc caggagagaa agaacggcag caacgccgca ttcgggtgct
      361 tcttgctcca ccacatagaa gcagcgccca attgcagcag tactgataac agaatgcctg
      421 accacagaat gggcttgcga ctcactctcg cgatggtcaa gaccccggcg aaccagagaa
      481 caggcgcgac aagccccaag aatcggaact caagttcaat cgaacctttc catcccaggt
      541 aggaacacaa cgcggcacag agacacgaca gcagtaccag gcaccaggaa atccatggtc
      601 tccagggacg cccacctacg ttgtcagcgg cggcattgct cgtgacactc gggagatcgg
      661 gactgtagct tctctcgaaa agaaggaaaa tcggtatcaa aagagttgcg aagacccact
      721 ccggcagtgc cacaggcaga gcccttcgga cgagaaaagc caccacgagg gaacctagaa
      781 ggaacagagg caccacatgc gatcttgtga aagcgatcaa ggattttcgt gtgggaatcc
      841 tcaaaagtct tctgtcatcc agccagagaa tccagtaaca cgcaagcatg aacaggagaa
      901 ggatgattgc cggtttgaga cggttcatcc tgaagtacag acaggccgct gccgttccca
      961 aggctgatac aacaaggctt ccatacgcgg gcagccagtg actgagaacc cggaagcgtt
     1021 gggcgcaaac gctcagcagt gcgagaggca tgacaggcag catccagaag atcag
//
LOCUS       DAOQUC010001611         1075 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_203707_length_1075_cov_3.831373, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001611 DAOQUC010000000
VERSION     DAOQUC010001611.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1075
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_203707_length_1075_cov_3.831373"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..597)
                     /locus_tag="PKH07_20330"
     CDS             complement(<1..597)
                     /locus_tag="PKH07_20330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37352.1"
                     /translation="MGDDSVKIHRGSLQEWESFANKSPQASFFHTPLWSHILTKAGLA
                     HTPEFLIFEFAGDLKAAVPALSVPHWAGLLQSWCSMPLGTYGGIMTNRHCSDSEWKNV
                     TAKVLQNLAPGIRQPSLCLDLPPALSIPSESLPDRAVVVDLSTHLLPLQEDFQSLWDS
                     RFKSNNRWSTRKAEKTGFQITELQGKEDMELFQMLYDGA"
BASE COUNT          249 a          268 c          287 g          271 t
ORIGIN      
        1 ggctccgtcg tacagcattt gaaagagttc catgtcctct ttcccctgca actcggtgat
       61 ctgaaatccg gtcttctctg ctttgcgtgt gctccagcgg ttgttgctct tgaagcgact
      121 gtcccaaagg gactggaaat cttcttgcag tgggagaaga tgtgtggaca aatccacgac
      181 gacagctcgg tctgggagcg actctgaggg aatcgagaga gcaggtggca aatccagaca
      241 caaagacggc tgacggattc cgggagcgag gttctgaagc actttcgctg tgacattctt
      301 ccattcagaa tcggagcagt gacggttagt catgatacca ccataagttc ccaggggcat
      361 ggagcaccac gactgaagca gtccagccca gtgcggaact gacaatgccg gtacggctgc
      421 cttgagatcg cctgcaaatt cgaagatcag gaactcggga gtgtgggcaa gtcccgcctt
      481 cgtcaagatg tggctccaaa gtggtgtgtg aaagaaagag gcctgtggac ttttgttggc
      541 aaaagattcc cattcctgaa gacttcctcg gtgaatcttt actgaatcgt ctcccatggg
      601 atacctctcg gctcacgaag cgcacaaccc gttctgaagc gaagtctcaa agtcgcctcc
      661 ttggaaaaca agtttcaaaa gaggctgtct ctgttgcgag cacacaccac acaactggcc
      721 tggcgtattt gccaagacgg tgctggcctg ctcatgagta cctgaattta tgtcacgaat
      781 cggcgtggaa agcaaatcta gccgtcattc ctgttctgtg gcttggtctt cttgacatcc
      841 agacggggta agactacctt tgcttggcat gaaggttgcg cactcaaagt gcgctcctga
      901 atgccgaatc gttttttcgg aggagcaaac ttgggatttc tttcgcgttc atatattctt
      961 tggcctctca ttttgttggt gatgctgctc ttggtgttca acatcaagac tgagcgccca
     1021 caggatatca gctacgacga attcctgacc aaactcaatg ctggtgatgt ggccg
//
LOCUS       DAOQUC010001612         1073 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_204488_length_1073_cov_2.156189, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001612 DAOQUC010000000
VERSION     DAOQUC010001612.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_204488_length_1073_cov_2.156189"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..744)
                     /locus_tag="PKH07_20335"
     CDS             complement(<1..744)
                     /locus_tag="PKH07_20335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HNT37353.1"
                     /translation="MQQETLFDSHAHLCHPKIWDHLDAIVQRADQSGVKGILCAGYDL
                     PSSERSLQAASRRSGVWAAVGFSPHDVSDLSESDWVTLESLLQQPKVVAVGETGLDYH
                     YMRSPASRQQDALRRHIRLAHKHGKPIILHLRESEDDLFSILDEEGIPEAGGVLHCFT
                     GTPETAELAVEKGLMVSFSGILTFSKSDEIVEALKRVPMERLLIETDCPYLAPVPYRG
                     KNCEPSMLPAVAEKVSEILGVPLVEVAERV"
     gene            complement(777..>1073)
                     /locus_tag="PKH07_20340"
     CDS             complement(777..>1073)
                     /locus_tag="PKH07_20340"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNT37354.1"
                     /translation="NNLPIKALMIEESRKGFELDPVRIRHELTESITLFGNLDTVSTL
                     RFGTPEQVRQETITQLQALSHGRFVMANGSPIAPETPRENVHTMIETTRGYRVP"
BASE COUNT          241 a          281 c          300 g          251 t
ORIGIN      
        1 gactctttct gcaacttcga cgaggggtac ccccaggatt tcggacactt tttcagcaac
       61 ggcgggaagc atggatggct cgcagttctt cccgcgatag ggaacaggag caaggtaagg
      121 gcagtcagtt tcgatcagca gtctctccat gggaacgcgt ttgagcgcct caacgatctc
      181 atcggacttg gagaatgtca ggataccgga gaaggacacc atcaggccct tctcaacagc
      241 caattcggcg gtctccggcg ttccggtgaa acaatgcagc actccacccg cctcgggaat
      301 tccttcctca tccagtatcg agaagagatc gtcttcagat tcgcgcaggt gcaagatgat
      361 cggcttgccg tgcttgtgtg cgaggcgaat gtgccggcga agagcatcct gctgtcgtga
      421 ggcaggggat cgcatgtagt ggtagtcaag cccagtctcc cccacggcga caaccttggg
      481 ttgctggaga agggattcca gcgtcaccca atccgattcg gaaaggtcgg aaacatcatg
      541 tggtgagaat ccgacagccg cccagacacc ggatcgtcgg gatgcagcct gcagactgcg
      601 ctcagaactg ggcagatcat atcccgcgca gaggatacct ttgacgcctg actgatcggc
      661 acgttggaca attgcatcga gatgatccca tatctttggg tgacaaagat gcgcatggga
      721 atcgaaaagc gtttcctgtt gcatgtgtga gaacctagtc agtcatactc gcgctgtcaa
      781 gggactcgat atcctcttgt tgtctcgatc atcgtatgca cgttctcgcg tggcgtttcg
      841 ggtgcaatgg gcgaaccgtt tgccatgaca aaccgaccat ggctgagtgc ctgaagttgc
      901 gtgatcgttt cttgtctgac ctgctcaggg gttccgaaac gaagcgttga caccgtatcg
      961 agattaccga acagcgttat tgactccgtc aattcatggc ggattctgac tgggtcaagt
     1021 tcaaaaccct ttcgactctc ttcgatcatc aaagccttga ttggtagatt gtt
//
LOCUS       DAOQUC010001613         1072 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_204624_length_1072_cov_4.408063, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001613 DAOQUC010000000
VERSION     DAOQUC010001613.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1072
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_204624_length_1072_cov_4.408063"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..641)
                     /locus_tag="PKH07_20345"
     CDS             complement(<1..641)
                     /locus_tag="PKH07_20345"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNT37355.1"
                     /translation="MKINKNIFTMDSRWRDTQWYLRQGNLFRHTLNRFRWYWYPRLRY
                     VARFPDHIDIEASSQCQLKCPMCFRHRLSISEGSMDFDLYRKLVDEAASFRIYSIKLS
                     WRGEPLLNPRLPEMVAYAKDKGIREVAFLTNGERMTADLAQKLIDAGTDWITFSFDGL
                     GSKYEEIRAPAKFDEAVERIRTMRQLRDKSKRTKPLIKVQTIWSAIQDNPGKY"
BASE COUNT          264 a          266 c          261 g          281 t
ORIGIN      
        1 ttgtatttgc cggggttgtc ctggattgcg gaccagatcg tttgtacctt tatcagaggc
       61 ttggtgcgtt ttgatttgtc gcgaagttgt cgcatggttc ggattcgttc aacggcttcg
      121 tcgaattttg caggcgcacg aatctcttca tacttgctgc caaggccatc gaaagaaaac
      181 gtaatccagt ctgtacccgc atcaatgagc ttctgggcaa gatcggccgt catgcgctct
      241 ccattggtta gaaatgccac ctctcgaatg cccttgtcct tggcataggc aaccatttcg
      301 ggaagtcgag gattgagcag tggctcaccg cgccaactga gtttgatgga gtagattctg
      361 aaagaggccg cttcatccac cagttttctg tacagatcaa aatccatgct tccttccgag
      421 atactcaggc gatgtcgaaa acacattggg catttcaact ggcattgaga tgatgcctca
      481 atgtcaatat gatcgggaaa ccgtgccacg tagcgcagcc tcggatacca ataccaacgg
      541 aaacgattca gagtgtgtcg gaacaagttc ccctggcgaa ggtaccactg tgtatcccgc
      601 catcgggaat ccatggtgaa gatgttcttg ttaatcttca tttgtgctca ctccgttctg
      661 aggctgatta aacacagcgt ggcaaaatgt acatagctga acagacccag actatcggac
      721 actgtcttca gcatgagaca ctctccaacg actgaaccaa tctcatagca ttaagataca
      781 acaactcaag gacacgacgc aactctggcg tcataaggac agcatcttca tggttcctat
      841 tcctctcttc gaccagcctt caaagaacct tgagtctgag tcattcaaag gctgggtttg
      901 cgttgacttc catcgttgaa ggaatatact tccagcgtgt tgtccagagt ggagaaactc
      961 aagaggcccg tgcctgcgaa caatatgatc cggaggttcg gtgaatatgc gagcagtccg
     1021 tcttagtggt gagaaagcgt ttgtcttgga gactttgcct gatccgactc cc
//
LOCUS       DAOQUC010001614         1072 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_204665_length_1072_cov_3.088496, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001614 DAOQUC010000000
VERSION     DAOQUC010001614.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1072
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_204665_length_1072_cov_3.088496"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..661)
                     /locus_tag="PKH07_20350"
     CDS             complement(<1..661)
                     /locus_tag="PKH07_20350"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HNT37356.1"
                     /translation="MKELRKKAAWKKRRIIYDNDGNEPVYFCEEATPESLLECRMKGL
                     IGTQVDTVLYCTWSSGFGYFTHDTKVGQVFDCTEEKFSNNRTREFLDKGLDPLTIVVN
                     FCHANNLEVFWSFRVNDTHDAAKTWYGPLMVPQLKKDHPEYLLGSKESRPPFGQWSSM
                     NFGLPEVRELAFRFVEEVCLNYDIDGVHLDFFRHAMFFRNPSMGLEASQEERDQMTEL
                     MG"
BASE COUNT          255 a          275 c          275 g          267 t
ORIGIN      
        1 gacccatcag ctcggtcatc tggtctcgct cttcttggct ggcttccagc cccattgagg
       61 gattcctgaa aaacatcgca tgtcgaaaga aatccagatg gactccgtca atgtcgtaat
      121 tcagacagac ttcctcgaca aaacggaagg ccagttcgcg gacctctggt aggccgaaat
      181 tcatggacga ccactgccca aagggaggcc tgctttcctt tgatcccaag aggtattcgg
      241 ggtggtcttt tttcaattgc ggaaccatca acggaccgta ccatgttttg gcagcatcgt
      301 gtgtgtcatt gaccctgaat gaccagaaca cttccagatt gttggcgtga cagaaattaa
      361 caacgattgt caggggatcg agtcctttgt cgagaaactc tcgagtcctg ttgttggaga
      421 acttttcttc agtacagtca aagacctgac ccacttttgt gtcgtgggtg aagtagccaa
      481 atcccgagct ccaagtgcag tagagaacag tgtccacctg cgtaccgatc agtcctttca
      541 ttcggcattc cagcaggctt tctggagttg cctcctcgca gaagtagacg ggttcattgc
      601 catcgttgtc atagatgatt cgacgtttct tccacgctgc tttctttcgc agttctttca
      661 tgggattgcc tccatacgat ttcggcggaa actctgcttg agaggaactc agccccgttg
      721 ccggagaact ttcccgcaag gcatggacag attccgtgca gacgacagat gagaccaatg
      781 ccagcgcaaa gagtatcccc aaccgaagtg cgcgtggttg aaaatgagga atcatggata
      841 tcatctgcat aacccttatt cttggactga accgagatac gataggacac tgaggacaag
      901 gggtcaagga tgtcaacggt ggcaggttgt tggctcagac actgttctgg cgcgcattcc
      961 aggtgcagac gcgacatcaa cctgtagcca gtttgagaac tccacgacga atactgccca
     1021 ggaatttccc gatggtggct tgccagaatc gggggcggat cgttcccatt tc
//
LOCUS       DAOQUC010001615         1072 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_204719_length_1072_cov_2.545723, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001615 DAOQUC010000000
VERSION     DAOQUC010001615.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1072
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_204719_length_1072_cov_2.545723"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1072)
                     /locus_tag="PKH07_20355"
     CDS             complement(<1..>1072)
                     /locus_tag="PKH07_20355"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF014156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HNT37357.1"
                     /translation="LEFLQRYAEEGIQLDNWWLDAGWYESEGSWVRTGTWVVDKKRYP
                     KGIREVSDYAKSLGIKTITWFEPERVHSGTWLAQERPEWVHGGKDGGLLKLGDSECRG
                     WMVDRIDKIVNEEGIDIYRQDFNIDPLVFWRANDTQDRQGITEIRHVEGYFKMWDELL
                     RRHPGMLIDSCASGGRRNDLETLRRAVPLLRSDYIFDAVGEQCHTYGLSFWIPFNGTG
                     FIEIEPYLVRSMMAAKFTLGSDVRRKDLNYNLLRKLIAEWREISDYFLGDYYPLTRYS
                     LAEDAWMAWQFNRVDLASGVVQAFRRKESTAFGSQFKLHDLEPEATYVVKNFDNDGRT
                     EEYSGAELMGSGLRIVIDERPAA"
BASE COUNT          249 a          315 c          239 g          269 t
ORIGIN      
        1 ctgcagcagg cctctcatca atgacgatgc gcaagccact tcccatcaac tctgcgcctg
       61 agtattcctc agtgcgtccg tcgttgtcga aatttttaac gacataggtc gcctctggtt
      121 ccaggtcatg cagtttgaac tgggatccga aggctgtgct ttccttgcga cgaaacgctt
      181 gaacaacgcc cgaggccaag tcaacgcgat tgaactgcca agccatccaa gcgtcttcgg
      241 cgaggctgta gcgtgtcaaa ggatagtagt cacccagaaa gtagtccgat atttctctcc
      301 attcggcgat cagcttcctt aggagattgt agttcagatc tttccttcgg acatcggaac
      361 ccagggtgaa tttcgctgcc atcatgcttc gaacaagata gggttcaatc tcaataaaac
      421 ctgtgccgtt gaagggtatc caaaaggaca gtccatacgt gtgacactgt tctcccactg
      481 cgtcaaaaat gtagtcgctt cgaagcagcg gaaccgctcg tctcagcgtt tccagatcat
      541 tgcgtctgcc accggatgcg caggagtcaa tgagcattcc gggatgacga cgcaacagtt
      601 catcccacat cttgaagtaa ccctcgacat gacggatctc tgtaatacct tgtctgtctt
      661 gtgtgtcatt ggcacgccaa aagacaagag gatcaatgtt gaaatcctgc cggtagatgt
      721 ctattccctc ttcattgaca atcttgtcaa ttcgatcgac catccagcct ctgcactcag
      781 aatcccccag tttcaggagc cctccatcct ttccaccgtg aacccattcg ggacgctctt
      841 gtgcaagcca tgtacccgaa tgcacacgct ccggttcgaa ccaggtgatg gtctttatgc
      901 caagcgactt tgcgtagtcg ctcacctctc tgatgccttt cggatatctc tttttgtcaa
      961 caacccaggt gcctgtgcga acccacgaac cctctgattc ataccacccg gcatccagcc
     1021 accagttgtc cagctgaatg ccctcttcgg cataccgctg cagaaactcc ag
//
LOCUS       DAOQUC010001616         1071 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_205006_length_1071_cov_2.858268, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001616 DAOQUC010000000
VERSION     DAOQUC010001616.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1071
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_205006_length_1071_cov_2.858268"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(470..1018)
                     /locus_tag="PKH07_20360"
     CDS             complement(470..1018)
                     /locus_tag="PKH07_20360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37358.1"
                     /translation="MRRLALVLLIASALGGVCYAFRQNLTEKYSRARYEIQCLFFLGT
                     PFRELTDAESFAVVECFENAIGLDVGLQREKTLGAHFGGKDLVRCIALLGCSDETLTI
                     LLSRMLATQDGDTPSLPEIREGSKYMLFDTGGEPDWWKPMDLTAPRYFKIYTQKDPPS
                     MSGYHLIISEKDRIVYFMQWST"
BASE COUNT          283 a          284 c          262 g          242 t
ORIGIN      
        1 caggacaaaa acctgcccct actttctaat ccctacgaga atccggcgac atgcaaacct
       61 ctccaatcga agggagagat tgagatttgc gtaagccgag caaacgctcg gcgttcccag
      121 gatccgcggt caccctcgcc ctttgggtga gggatgcggt gagggaaatc agtggcgttg
      181 gcatccctgc cgacgacgtg agcacagagg agatcatcgg caagaatgcc tgtgttacat
      241 ccagaagcag tcctcaacga ggagggtgtc tcaaaaccca ttagagcaac accttctgtc
      301 attctgaacg gagcaaagcg aagtgaagaa tctcgattct atggggcttt cagcgatcag
      361 agagatgctt cgcttcgctc agcatgacaa cccaaagagt gttttgagac accctccgag
      421 ttgaaggctg ccacacggga aggagaggga acccggcaca ctcgactcac tacgtgctcc
      481 attgcatgaa ataaacaatc cggtcttttt cggagatgat aagatgatac ccgctcatag
      541 atggaggatc tttctgagtg tatatcttga agtacctggg ggcagtaaga tccatgggtt
      601 tccaccaatc aggctcaccg cctgtgtcaa ataacatata cttgctgcct tctctgatct
      661 caggaagact tggagtgtcc ccatcctgtg tggcaagcat gcgactcaga agaattgtga
      721 gcgtttcgtc cgagcacccc aataacgcga tacagcgcac cagatccttt cctccaaaat
      781 gtgcaccaag tgtcttttct cgctgtagac caacatccaa tccgatggcg ttttcgaagc
      841 actcaacaac cgcgaaagat tctgcatcag tcagctccct gaaaggcgtg cccaggaaga
      901 acaagcactg gatctcatat cttgcgcgac tgtacttttc tgtaaggttc tgacgaaaag
      961 catagcacac tccgcccagt gctgaggcaa tcaataagac gagtgccagt cttctcatgg
     1021 tctggtcatt ctatcgcgaa agatatcgta caccgtctcg gaatctgtag c
//
LOCUS       DAOQUC010001617         1071 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_205021_length_1071_cov_2.727362, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001617 DAOQUC010000000
VERSION     DAOQUC010001617.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1071
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_205021_length_1071_cov_2.727362"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1071
                     /locus_tag="PKH07_20365"
     CDS             <1..>1071
                     /locus_tag="PKH07_20365"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HNT37359.1"
                     /translation="MQDVAFRVGVDARPLHEPPCGIRRYLQNLLRALLKLDNATGYVL
                     FTNRPLPELSANNEERVQISTLGGSRQALMRPWWEVLTLPRAIRKSSVDLFFSPMGIV
                     PARCLVPSVVTVHDLAFMKYSGIQPWNYRMYWNWVMKRVPNADAIIAVSHSTKSDIEQ
                     LLPVQPERVHVIWEGLDESFRSPTTHEERQSLRIKYGLKGDFVLFVGTREPRKNLNTL
                     VKAMSVLNETRTDPVPLILVGPEGWGNQGDPQESGTWVRVLQPLSDGELKTLYSCATV
                     FAFPSLYEGFGLPLLEAMSCGAAVIASNVASIPELAGDSALLVNPTDVAEWTRGLALL
                     LKDGQLRSSLVQKGHSQVERFA"
BASE COUNT          235 a          296 c          283 g          257 t
ORIGIN      
        1 ggatgcagga cgtggcattt cgtgttggcg ttgacgcacg acctctgcat gaacctcctt
       61 gcggcatcag acgctacctt cagaaccttc tcagagccct tctgaaactc gacaacgcaa
      121 ccggctatgt gctcttcacc aatagaccgc ttcccgagct ttcggccaac aacgaagaac
      181 gcgtccaaat aagtaccctc ggtggatcgc gacaggcact catgcgcccc tggtgggaag
      241 tgctcacact gcctcgggcg atcagaaaga gttctgttga tctttttttc tctccgatgg
      301 gaatagtccc tgcccgctgt ctggttcctt cggtcgtgac cgtccatgat cttgcgttca
      361 tgaagtattc aggcatccag ccttggaact acagaatgta ctggaactgg gtcatgaaaa
      421 gagttccaaa tgcggatgcg atcattgctg tgtctcattc cacaaagtct gacatcgagc
      481 agctgttgcc agtgcagccc gaacgagttc atgtcatctg ggaaggtctg gatgagtctt
      541 tcaggagccc aacaactcat gaagaaaggc aatcccttcg tatcaagtac ggcctcaaag
      601 gggattttgt tcttttcgtc ggcacccgag agccacgcaa gaacctgaac actctggtca
      661 aagccatgtc tgtcctgaat gaaacacgca ccgatccagt acctttgatc cttgtgggtc
      721 ctgagggttg gggcaatcag ggcgacccgc aagaatctgg cacctgggtg agagtgctgc
      781 agccactctc agacggcgaa ctcaagacac tttattcctg tgcaacagtg tttgcgtttc
      841 cctccctcta cgaggggttc ggtcttcctt tgctggaagc gatgagctgt ggcgctgcag
      901 taattgcatc caacgttgca tcaatccctg aactggccgg tgattccgct ttgctggtca
      961 atccgacaga tgtcgcagag tggacacggg ggctggcact cctgctgaag gacgggcaac
     1021 tgcgcagttc gcttgtgcag aaagggcact cccaagtcga gcgcttcgct t
//
LOCUS       DAOQUC010001618         1071 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_205069_length_1071_cov_2.350394, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001618 DAOQUC010000000
VERSION     DAOQUC010001618.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1071
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_205069_length_1071_cov_2.350394"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            13..174
                     /locus_tag="PKH07_20370"
     CDS             13..174
                     /locus_tag="PKH07_20370"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37360.1"
                     /translation="MLEILGSAYSRGCNLLLNTAPLPDGSLDPEDAEVLLEVGRRLHN
                     GELSQFLRD"
     gene            complement(156..>1071)
                     /locus_tag="PKH07_20375"
     CDS             complement(156..>1071)
                     /locus_tag="PKH07_20375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37361.1"
                     /translation="MGLVERSAELHTRDTKTPHLLNIDNTYKPENWYMYAQLPDVLCA
                     DPYFQGELESACFEKPERLESHSKPTYVLAASTICAQSCAPKPLHIILQSCRHERSDG
                     KVFRFPTPEEKRVELYYALLAGAKGFSYWWYTPHNEFHGCGTDLPEAKPLYEEIGRLG
                     AEVRSVGDLITRSCPAHLETIAPRDVKTAVLLVGMDTMILLCVNENIRCHLMGTDVTP
                     VEDVKIECRIPKWLKDPVAFEVTWQGTKDVAFENNQDAMRLELGVLSLTRMVVITNNQ
                     QLRSEIQSRYLEEFAEKIALLTAQSLRN"
BASE COUNT          232 a          303 c          289 g          247 t
ORIGIN      
        1 accgttgacg aggtgcttga gatactgggt tcggcataca gcagaggctg caacctgctt
       61 ctcaacacag ccccactccc cgacggcagc cttgacccag aggatgccga ggtactgctc
      121 gaagtcggca gaagacttca caacggagag ctgtctcagt tcctcaggga ctgagctgtg
      181 agcaaggcga tcttctcagc gaattcttcg agataccgac tctgtatctc actgcgcagc
      241 tgctgattgt ttgtaatcac aaccatccgt gtgaggctca acacaccaag ttcgagcctc
      301 atagcgtcct ggttgttctc gaatgccacg tctttggttc cctgccaggt cacctcaaat
      361 gcaaccggat ccttcagcca cttggggatg cggcactcga tcttgacatc ctcgactggg
      421 gtgacgtcgg ttcccatcag gtgacagcgg atgttctcat tgacacacag aagaatcatc
      481 gtgtccatac cgacaaggag cacagccgtc ttgacgtctc tgggagcgat ggtctccaag
      541 tgcgcaggac agctccttgt gatcagatct ccgacactcc tgacttccgc tcccaagcga
      601 ccgatttcct catagagtgg cttggcttca ggcaagtcag ttccgcaacc gtgaaactca
      661 ttgtgcggcg tataccacca gtaagagaat cccttcgccc ctgccaggag cgcgtagtag
      721 agttcaacgc gcttctcctc aggagtgggg aatcgaaaca ccttgccgtc agaacgttcg
      781 tgacggcatg actgcaggat gatatgcagt ggcttggggg cacaggactg cgcgcagatc
      841 gtcgaggctg ccaagacgta agtgggcttt gaatgacttt cgagacgctc tggcttttcg
      901 aaacacgctg actccagttc cccttggaaa tagggatctg cgcagagcac gtccggcagc
      961 tgtgcgtaca tataccagtt ctcgggcttg taagtgttgt caatgttcag cagatgaggc
     1021 gtcttggtgt cccgggtatg cagttccgcg ctgcgctcta caagacccat t
//
LOCUS       DAOQUC010001619         1070 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_205310_length_1070_cov_3.291626, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001619 DAOQUC010000000
VERSION     DAOQUC010001619.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1070
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_205310_length_1070_cov_3.291626"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            23..>1070
                     /locus_tag="PKH07_20380"
     CDS             23..>1070
                     /locus_tag="PKH07_20380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37362.1"
                     /translation="MSLHGALSLGYFLFSAWLFSLQSVWLGAKRIRGSAVEDVPTQQD
                     DPGQADSQRDLDFPLAMLAGFVGVFVAMAFSPKPYCALTASLLATLVSMMALGVGHWI
                     PYAAAGVPLVWAHWQLWMLVETPQVVWTNAVVTIAITIGATLALSLFGHSGRVGSQRS
                     LIAVEKFLHVLWLLTLQAAFFKTLDPHQYFLACSVISLAALLFSLLPALKNLTDFSFL
                     PMLCCLFSLAWNEFLKLCKEIGYTRLSIRLDTDFLEAHWALWLATFIAFGYACAAAHI
                     KRLGNNRAFLRESNAYQGLHILLAMGIGLYCFASVYKGENLVLCLGLASVIVALLSRW
                     PGIRQALLLSDLYLI"
BASE COUNT          205 a          287 c          272 g          306 t
ORIGIN      
        1 gttcaaggaa cctggtgata tcatgagcct tcatggagca ctctccctgg gctattttct
       61 cttttctgcc tggctcttca gtcttcaatc agtatggctc ggcgcaaaac gcatccgagg
      121 atcagcggtt gaagacgtgc cgacgcagca agatgatcca ggacaggcag atagtcagag
      181 agacttggat tttcctctgg caatgctggc cggatttgtc ggagtttttg tcgccatggc
      241 tttctctccc aaaccctact gcgccttgac tgcaagcctt ctggctaccc ttgtgtcaat
      301 gatggctttg ggcgtgggac actggatccc ctatgcggct gcgggagtgc cactcgtttg
      361 ggcacattgg caactgtgga tgctggtcga aacaccgcaa gtggtatgga caaatgccgt
      421 cgtaacaatc gcgatcacga ttggcgctac gctcgcactg agtctgttcg gccattcggg
      481 aagagtgggt tctcagagat cactcatcgc tgtggagaag ttcctgcatg tcttgtggct
      541 tctgacactt caggccgcct tcttcaaaac gcttgatcct catcagtact tcctggcttg
      601 ttccgtgatc agtcttgcag cccttctctt ttcactgctc cccgctttga agaatctgac
      661 cgatttttcg ttcctgccca tgctttgctg tctgttttcc ctggcgtgga atgagtttct
      721 gaaattgtgc aaggagatcg gatatacccg tttgagcatt cgcctggata ccgatttcct
      781 ggaagcgcac tgggcactct ggttggcaac cttcatcgcc tttggatatg cctgtgcggc
      841 cgcccacatc aaaagacttg gaaacaacag agcctttctt cgagagtcaa atgcctatca
      901 aggtctgcat atcctgctcg caatgggcat cggtctttac tgttttgcgt ctgtctacaa
      961 gggcgagaac ctcgtactct gccttggact tgcctctgtc atagtggctc tgctcagccg
     1021 ttggccagga attcgacaag cactgttgtt gtcagacctc tatctgattc
//
LOCUS       DAOQUC010001620         1070 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_205424_length_1070_cov_2.443350, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001620 DAOQUC010000000
VERSION     DAOQUC010001620.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1070
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_205424_length_1070_cov_2.443350"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..708
                     /locus_tag="PKH07_20385"
     CDS             <1..708
                     /locus_tag="PKH07_20385"
                     /inference="COORDINATES: protein motif:HMM:NF025888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37363.1"
                     /translation="DPKSLFLRCVILVGEPWDGGIQREVMQRLLVKVYGAYGLDEVFN
                     PGIAFEDGEQHCLLVNEDQFLLEIVDTKEGTVLGEGQQGELLITTLTKEALPLIRYRT
                     GELGCLQYVTDNNMQRLALKLLGKRRDDLVHFNGAKFYPPQLGEVLRTVIGKEIPFSV
                     VLTRSEKGDRAELWVEMCELAFAREMVGITHIKDRLESMLFLRLGVTVHVRWMEQGAL
                     QGVPAIQDNRTAFAIPQ"
     gene            complement(824..>1070)
                     /locus_tag="PKH07_20390"
     CDS             complement(824..>1070)
                     /locus_tag="PKH07_20390"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNT37364.1"
                     /translation="GNEIWSVQADVGRNPFSPTESNGKVYVCGNKGLLQCFQASDGLK
                     LWEYQTTPQLYVYSSVTVEDEIAYVSGMDGTVVAVKP"
BASE COUNT          244 a          269 c          294 g          263 t
ORIGIN      
        1 gatccgaaat cactgttctt gcgttgcgta atccttgtcg gagaaccttg ggatgggggt
       61 atccaaaggg aagtgatgca aaggcttctc gtcaaggtct atggtgccta tggtcttgat
      121 gaggtcttca acccaggaat agcgttcgag gatggggagc aacactgcct tctggtcaac
      181 gaagatcagt ttctgcttga aatcgtcgac accaaagagg gtacagtcct gggggaaggg
      241 cagcagggcg aactgctcat aacaaccttg accaaagagg ctcttccgct gatccgctat
      301 cgtacgggtg agcttggctg cctgcaatac gtgaccgaca acaatatgca gagacttgcc
      361 ttgaaacttc tcggcaagcg tcgggacgat ctggttcatt tcaacggagc caagttctac
      421 ccgccccagt tgggagaagt actacggaca gtcattggca aggaaatccc gttctccgtc
      481 gtgcttactc ggagcgagaa gggagaccgc gcagagttgt gggttgagat gtgcgaactg
      541 gcttttgctc gtgaaatggt gggtatcacg catatcaagg atcggcttga gtccatgctt
      601 ttccttcggc ttggtgtgac ggttcatgta agatggatgg agcagggcgc tctccaggga
      661 gttccagcga tccaggataa ccgcactgcg tttgcgatcc ctcaatgagc atcgcccaag
      721 ggagaggctc acgttctgct ctctgtctga gacctggttc ttcggcgaga ctatctatgt
      781 tgatcgaaga gtctcgcctc attcggcatg atgttcgaac gccttacggt tttactgcaa
      841 caacggttcc gtccattcct gaaacatagg caatctcatc ttctactgtc accgacgaat
      901 acacgtagag ttgcggcgtt gtctgatact cccacagttt cagaccatcc gaagcctgga
      961 aacactggag caagcccttg ttgccgcaga cataaacttt gccgttgctt tcagtcggag
     1021 aaaatggatt gcgaccgacg tctgcctgta ccgaccagat ctcattcccg
//
LOCUS       DAOQUC010001621         1069 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_205657_length_1069_cov_2.947732, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001621 DAOQUC010000000
VERSION     DAOQUC010001621.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_205657_length_1069_cov_2.947732"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..558)
                     /gene="pyrE"
                     /locus_tag="PKH07_20395"
     CDS             complement(<1..558)
                     /gene="pyrE"
                     /locus_tag="PKH07_20395"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012268739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HNT37365.1"
                     /translation="MSHNDLLSLLSQTGAWCKGHFLLSSGRHSDQYIQCARLFENPLH
                     GEIFGTALAREFQNCVVDVVVGPALGGILLAYDVGRSLGKRVLFAERADGKMSFRRGF
                     CIKPGEKVLVVEDVVTTGGSVQEVAELVRQSGGNVVGIGSIVLRRSPGVDLDFSVKTL
                     LEFEVPSYAPDSCPLCSQGIPITKPG"
     gene            770..>1069
                     /locus_tag="PKH07_20400"
     CDS             770..>1069
                     /locus_tag="PKH07_20400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37366.1"
                     /translation="MQMASNEDATPSRWDSFILALLVLISGSAFFIFVTRPPEFWLAF
                     PSLLRIFLEHDWLGKWPAASFWTTLLCSVVGYVCYAAVGSNLIRSLHLTRSRSVFL"
BASE COUNT          245 a          292 c          257 g          275 t
ORIGIN      
        1 tcccggcttt gtgatcggaa taccctgact gcaaagaggg caagagtcgg gtgcatagga
       61 cggcacctca aattcgagaa gtgtcttgac cgaaaaatca agatccacac ccgggcttct
      121 tcttaggacg atggagccaa tgccaacaac gtttccgccg gactgcctta ccaactcagc
      181 aacttcctgc acggaacctc ccgtggtgac cacatcctcg accaccagaa ccttttcacc
      241 gggtttgatg caaaaacctc ttctgaacga catctttcca tcggcgcgtt cggcaaacaa
      301 gactctcttg ccaaggctcc ttccgacatc gtatgccaac agaattccac cgagggctgg
      361 cccaacgacc acatctacta cacagttttg gaactccctc gccagtgcgg taccaaatat
      421 ctctccatgt aacggatttt cgaacagccg agcacactga atgtactgat cactgtgccg
      481 tccggaactc agaagaaaat gccccttaca ccaagcgcct gtttgactca agagactcaa
      541 gagatcattg tgactcaagg ctgatatcct cccaaaatga ttcttcggca acgacaaatc
      601 tacgggtagc cccgcgggtt tgcgtagcaa catctctgac tggttttgat tatggtggaa
      661 tcgaaaggct tcggcaagta gaatcactgc ggtttttggc gttgaacgcg gatcgcattt
      721 ggtctagcgt tgccaaatgg tattaacgaa ctgggaaggt ttgcactgaa tgcagatggc
      781 aagcaacgag gatgctactc cttcgcgctg ggattcattc atcctggctc tgctcgtgct
      841 gatttctgga agtgcgtttt tcatctttgt gacaagacct cctgaattct ggttggcttt
      901 cccctcgctg cttcggatct tcctcgaaca cgattggctg gggaaatggc cggcagcgtc
      961 cttttggaca acgttgctct gctccgttgt tgggtacgtg tgctatgcgg cagtgggttc
     1021 gaatctgatt cggtctttgc atctgactcg ttccagatcc gtattcctt
//
LOCUS       DAOQUC010001622         1069 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_205770_length_1069_cov_2.091716, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001622 DAOQUC010000000
VERSION     DAOQUC010001622.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_205770_length_1069_cov_2.091716"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1069)
                     /locus_tag="PKH07_20405"
     CDS             complement(<1..>1069)
                     /locus_tag="PKH07_20405"
                     /inference="COORDINATES: protein motif:HMM:NF019368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HNT37367.1"
                     /translation="QNHGHDSIAGCSRDIVHEDMMFRFRQVREISTCVTERAMMDIVG
                     CIDLSAWSSEDMAVVVFNPATFTRSETIKTVLEIPLEWKATGFEIVDEKGGKVPLQIT
                     DQQSPFHQIVQSPNDVANNFPSTRYHALAKFDGVPPLGYRTFRVKPLHSAKKPKQPES
                     MLTGTQSMENEFLSVTINANGTLDILHKETGRLFKNLGYFRDSSERGNPWEHHAVPND
                     VILTTLSHPTRIALVRDGSLETSFRVSIDWALPEGRSADEKTRGETFRRYPITNTVTL
                     RKGQPWVEVVTELDNTVEDHYLQVCFPTETVTDKVHVQGHFDVVERPVKKPDYSKYDE
                     EPMTEHPTNSFVDLSDGLHGFA"
BASE COUNT          232 a          271 c          291 g          275 t
ORIGIN      
        1 ggcgaacccg tgcagaccat cactcaagtc cacgaaggag ttcgtcggat gctccgtcat
       61 aggttcttcg tcgtacttgc tgtagtcagg tttcttgaca ggcctttcca caacgtcaaa
      121 atgaccctgc acatgaacct tgtctgtgac agtttccgtt gggaagcaga cctgcaggta
      181 gtgatcttcg acggtgttgt ccaattcggt gaccacttca acccagggct gccccttgcg
      241 aagtgttacc gtgttggtga tgggatatcg tcggaaggtc tcacctcggg tcttctcatc
      301 ggctgaacgc ccttctggaa gcgcccagtc aatgctcact cggaatgagg tctccagact
      361 cccgtctcga accagggcga tccgtgtcgg gtgactgaga gtggtgagga tgacgtcgtt
      421 gggaacggca tggtgttccc agggattgcc tctctcactt gaatcgcgga agtatccgag
      481 gtttttgaag aggcggccgg tttctttgtg caggatgtcc aatgtgccat ttgcgtttat
      541 tgtcacactc agaaattcat tttccatgga ctgtgtgccg gtcaacatgg attcaggttg
      601 tttcggcttc tttgccgaat gcagaggctt gactcggaac gtccggtagc cgagtggggg
      661 aacaccatca aacttggcca aggcgtggta tcgggttgaa gggaaattgt tggcaacatc
      721 gttgggactc tgaacgatct gatgaaacgg ggactgttga tcggtgatct gaagtggcac
      781 ttttccgccc ttttcatcca cgatctcgaa acccgttgcc ttccattcca aaggaatctc
      841 aagcacagtc ttgatggtct ctgagcgagt gaaagttgcc gggttgaaaa cgactacggc
      901 catatcttca gacgaccagg cacttagatc aatgcaacca acgatatcca tcatggcacg
      961 ctctgtgacg caagtactga tttcccgcac ctgccgaaat cgaaacatca tgtcctcatg
     1021 gacaatgtcg cgactgcaac cggcaatcga gtcgtggcca tggttttga
//
LOCUS       DAOQUC010001623         1068 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_206080_length_1068_cov_2.114511, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001623 DAOQUC010000000
VERSION     DAOQUC010001623.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1068
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_206080_length_1068_cov_2.114511"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..365)
                     /locus_tag="PKH07_20410"
     CDS             complement(<1..365)
                     /locus_tag="PKH07_20410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase"
                     /protein_id="HNT37368.1"
                     /translation="MTEKPLGIYVHVPFCRRKCFYCDFLSWAERESDLERYVSCLEDE
                     IVRKHLWQGVAGASVASVYFGGGTPSLLTPQQIEVLLKCIRGHFSLQSGCEITVEVNP
                     ESASQRFLEMAHQLGVNRL"
     gene            complement(362..>1068)
                     /locus_tag="PKH07_20415"
     CDS             complement(362..>1068)
                     /locus_tag="PKH07_20415"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNT37369.1"
                     /translation="SVWKQAQDNGDMRPFQNQIMGWWWTPTRPDQVGINFTHINFIDG
                     TKAEDLTRATIEGRKQAYECIEVFRKYVPGMEECYMISTPNTVGVRETRRILGDYLLT
                     KEDLLAKKTFSDSIGYGSFFIDIHCVSGPGMDQKTYRPEPGFKYQIPYRILLPQKVEN
                     LLVAGRCASCTHEALGSLRVMPQCGVMGQAAGVSAALSLRTSVSPRQVDVGSLQSLLR
                     EQDCIVEESDIVKANT"
BASE COUNT          263 a          293 c          264 g          248 t
ORIGIN      
        1 ctcaaccggt tgacccccaa ttgatgagcc atctcaagga aacgttgtga ggcgctttcc
       61 ggattgacct ccacggtgat ctcacagccg ctttgaagtg aaaaatgccc gcgaatgcac
      121 ttcagaagca cttcgatctg ttgtggtgtc aaaagactgg gagtaccgcc gccaaaatag
      181 accgaagcaa ccgaagcgcc tgcgacaccc tgccacaagt gcttgcgaac aatctcatct
      241 tcaaggcagg aaacatatcg ctccaagtcg ctttcgcgtt cagcccacga gagaaagtca
      301 cagtagaagc acttccttcg gcagaaagga acatgaacgt agatccccag aggcttttcc
      361 gtcatgtgtt cgccttgaca atgtcggatt cttctacgat gcagtcctgc tcgcgaagca
      421 atgactgcag agaacccaca tcaacctgtc gtggggacac gcttgttcga agcgacagag
      481 ctgcggaaac tcctgcggcc tgccccataa caccgcactg aggcatgacg cggagactgc
      541 ccaaggcttc atgggtgcag ctggcgcagc gaccggcgac cagaaggttc tcaaccttct
      601 ggggcaacag aattcggtaa ggtatttgat acttgaatcc tggttccgga cgatatgtct
      661 tctgatccat gcctggccct gagacacaat ggatatcaat aaagaatgaa ccataaccga
      721 tggaatcgga gaaagtcttc ttggcgagca gatcttcttt tgtcagcaga tagtctccca
      781 gtattcttcg ggtctcacgg acaccgaccg tgttgggtgt agagatcata taacactctt
      841 ccatgcccgg gacatatttg cgaaagacct caatgcactc atacgcctgt ttgcgtccct
      901 cgatggttgc acgagtcaaa tcctctgcct tggtaccatc aatgaagttg atatgcgtga
      961 agttgattcc gacctgatcc ggtcgtgtgg gtgtccacca ccatcccatg atctggttct
     1021 gaaaaggtct catgtcgcca ttgtcctgcg cctgcttcca gacggatt
//
LOCUS       DAOQUC010001624         1064 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_207282_length_1064_cov_2.838454, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001624 DAOQUC010000000
VERSION     DAOQUC010001624.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1064
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_207282_length_1064_cov_2.838454"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..997)
                     /locus_tag="PKH07_20420"
     CDS             complement(<1..997)
                     /locus_tag="PKH07_20420"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNT37370.1"
                     /translation="MLVQTREAMSEAGLHRLLGKQASWKAVTPAPKACVFSVQTQSGE
                     SLSSLITHFRNQPEVLYAEPNFIYRLAETTPNDTYFASEQWALRNTGQKGGISGEDID
                     ATEAWDTQTGSPDVVVAVIDTGLALEHSDIRNGTGNVWLNTDEIPGNLLDDDNNGYVD
                     DWRGWNFFEENNNPNDQHSHGTHVSGTIGAIGNNGQGVTGVCWQVKIMPLAAFNPRTG
                     EATNEDLVAAIQYAVNNGAKVINASWGDFVHSQILEDAVRTARDAGVLFVAAVGNNSL
                     LDLEHASFYPAGYQLENIVSVASSNSSARISSFSNQGNYSVDLFAPGESIFNLSLN"
BASE COUNT          239 a          282 c          287 g          256 t
ORIGIN      
        1 tgttcagact gagattgaag atagactccc ccggtgcgaa cagatccact gagtaattgc
       61 cctggttaga gaaagaggaa atccttgccg acgagttgga ggaagccacg gagacaatgt
      121 tctccaactg gtatcctgcc gggtagaatg aggcgtgttc caaatcgagc agagagttgt
      181 tcccaacggc ggccacgaag agcactcctg catcgcgagc agtgcggact gcgtcctcca
      241 gaatctgtga gtggacaaag tccccccaac tggcgttgat caccttggct ccattgttga
      301 cggcgtattg gatggctgcg accaggtctt cgtttgttgc ctcacccgtc ctgggattga
      361 aggctgccaa aggcatgatc ttgacctgcc agcagacgcc ggtcacaccc tggccgttgt
      421 ttcctattgc accgatggtg ccggaaacat gggttccatg cgagtgttga tcattgggat
      481 tgttgttttc ttcaaagaag ttccatcctc gccagtcatc cacatagcca ttgttgtcgt
      541 catccagcaa gttgccagga atctcatcag tgttcagcca tacgtttcct gttccgttgc
      601 gtatgtctga atgttccaat gccaggcctg tgtcaatgac agcgaccaca acatcgggtg
      661 atcctgtctg cgtatcccag gcctctgtgg catcaatatc ttcgcccgat attccaccct
      721 tctgacctgt gttgcgaagt gcccactgtt ctgaggcaaa ataagtatcg ttgggagtgg
      781 tctcagcgag gcgatagatg aagttgggct cagcgtagag cacctcaggt tgattcctga
      841 aatgagtgat cagacttgac aaactctccc ctgactgtgt ttgtacagag aagacgcatg
      901 ccttgggagc gggggtaacg gctttccagg aagcctgctt tcccaaaagc cggtgaagac
      961 ctgcttcaga catcgcctca cgggtctgca cgagcactga gccttctttc cacgctcctg
     1021 tggcagtgga ggcaccaaga aggaacaagc caaagagcag cagc
//
LOCUS       DAOQUC010001625         1063 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_207752_length_1063_cov_1.799603, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001625 DAOQUC010000000
VERSION     DAOQUC010001625.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1063
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_207752_length_1063_cov_1.799603"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(435..>1063)
                     /locus_tag="PKH07_20425"
     CDS             complement(435..>1063)
                     /locus_tag="PKH07_20425"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNT37371.1"
                     /translation="ISLGGIGVDPRQVWGGRGDRFRQKYRISSPIVLQLSKQIIDKGS
                     QHTVEAMKLLWSRGCDATLVLAGQVTENFDTYWFKQPAEVLERTVLMDYVTQEEKKDL
                     LDACDVFVMPSKAESFGLVYCEAWLYKKPVIGAFAGGVPALIEDGKDGFLVPYGDAHF
                     LGETILGLLERPALRQALGEVGYRKTLRDYTWEVQYQNVLRVYEYLTS"
BASE COUNT          283 a          331 c          210 g          239 t
ORIGIN      
        1 agcgtcagac ccatcgcata gtacgatcgg ccgattcgga acatggcatc ctcagcatag
       61 gctccattgg gacgctcact caggtatgcc cgataccgaa gaaccgcacg accatgccga
      121 ccctcattgt aaagatcgtc ggctcctttc aggggatggg tcatgcaacc caatccgaac
      181 aagcacagca acaaatacgg agaaagtctc ctcatatcct gttcaccaat ccagactgca
      241 tattcttaac aaccatctca ttcgtatctc aactctgaag actatctcaa attctgacac
      301 gacaatcaaa cctaaaatcc acaaaacccc taaacggcgt cctaaactct cgacgggggc
      361 tttcggggga ttattcactc cattccatct tttcagaaaa aaaaagcgta tccgcacaca
      421 aaaggggtct tttgtcatga agtcagatat tcatagacac gcaagacatt ctgatactgc
      481 acttcccaag tgtagtcccg tagcgtcttt cgatacccca cttctcccag cgcttgcctg
      541 agtgcaggcc tctccagcaa gccgaggatc gtttcaccga gaaagtgggc atctccgtag
      601 ggaacaagaa atccatcctt tccatcttca atcagcgctg gcacaccccc tgcaaaggct
      661 ccaatgaccg gcttcttgta aagccacgcc tcgcagtaca ccagtccaaa agactccgcc
      721 ttggaaggca tgacaaacac atcacacgca tccaagagat ccttcttctc ttcctgcgtg
      781 acatagtcca taagaactgt cctctcaagg acttcggctg gctgtttgaa ccagtaagta
      841 tcgaagttct cagtgacctg ccccgccagg acaagggtcg catcgcagcc ccgactccac
      901 aggagtttca tcgcctcaac cgtatgctga ctccctttgt caatgatctg tttggacaac
      961 tgaagcacga tcggactcga tattcgatac ttctgacgga atctatctcc cctgccgccc
     1021 cagacctgcc tgggatcaac gccaattccc cccagactga tct
//
LOCUS       DAOQUC010001626         1061 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_208189_length_1061_cov_3.193837, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001626 DAOQUC010000000
VERSION     DAOQUC010001626.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1061
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_208189_length_1061_cov_3.193837"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..876
                     /locus_tag="PKH07_20430"
     CDS             <1..876
                     /locus_tag="PKH07_20430"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNT37372.1"
                     /translation="EMSAGVSIYRRPSGLMQSANTLGLYLLCLLPLSLSALFWTGSIF
                     ARLFCLGTFAAGTMALIITYSRGSWLGFIFGSLVFFLWLLRKKLITYRRHFHLLALFA
                     LLVMMILVIMWPKIMDRLLHTPSIPIHTRTFLTRLCLKVCATHPLLGVGLNTSAETAT
                     RMILTVRDPHNIIDYVMANPLVHNLYLLMLSEIGLLGLSVFLCLLVALFRQALKAIQI
                     QDRLAMSVGVAIYSALSGFLVAELFDFSYRLDQVFYLFWALSGLIVAINRRQTHLDST
                     ASTDAEEQYAVTPSG"
BASE COUNT          212 a          290 c          259 g          300 t
ORIGIN      
        1 gagatgagcg cgggagtttc catatatcgc agacccagcg gactgatgca gagtgcaaac
       61 actcttggac tatatctgct ctgtcttcta ccattgagtc taagcgcgct tttctggacc
      121 ggaagcattt tcgccaggct gttctgtctt ggcacttttg ccgcaggcac catggcgctg
      181 atcatcactt actctcgggg cagctggctg ggcttcatct ttggctcttt ggttttcttc
      241 ctgtggctgt tgcggaaaaa actgatcacc tatcgaagac atttccattt gcttgcgctc
      301 ttcgcgttgc tggtgatgat gatcctggtc attatgtggc ccaagatcat ggatcgcctt
      361 ctgcatacgc cgtccatccc cattcataca cggacattcc tcactcggct ttgcctgaag
      421 gtgtgcgcaa cccatccatt gctgggtgtt ggtttgaaca cttcggcgga gacggcaact
      481 cgtatgatct tgacggttcg ggatccgcac aacatcatag attacgtcat ggcaaatcct
      541 ctggtgcaca atctctacct tctcatgctg agtgagattg gcctattggg gctttctgtc
      601 tttctttgcc tcttggtggc cttgttcaga caggctctga aggccataca gatccaagat
      661 cgactggcca tgtcggtggg tgttgcgatc tattccgccc tctcaggatt cctggttgca
      721 gaactttttg acttctccta ccgcctagac caagtcttct acctgttctg ggcgctcagt
      781 ggcttgatcg tcgcaatcaa caggcgacag actcatcttg atagtactgc atcaacggat
      841 gctgaggagc aatacgctgt taccccctca ggataacgcg cgacagaacg aagttgaaac
      901 tcaacaacag agaattgcgc tgcgatctct gccagttcgc gcttggcttg ccttcacctc
      961 gcgctgtaat ctcaattgcc tgcactgtcc ccgtgggagt gtttcagatc gtcctcgctc
     1021 agaagtggac atacagccat ccctgtttca gaaaatcgag a
//
LOCUS       DAOQUC010001627         1060 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_208586_length_1060_cov_2.610945, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001627 DAOQUC010000000
VERSION     DAOQUC010001627.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1060
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_208586_length_1060_cov_2.610945"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..193)
                     /locus_tag="PKH07_20435"
     CDS             complement(<1..193)
                     /locus_tag="PKH07_20435"
                     /inference="COORDINATES: protein motif:HMM:NF016205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HNT37373.1"
                     /translation="MNEDLLLDPERVESRLKWSKLLDIYGQLLPDKQKVFLALHYDED
                     LSLSEIAEMHHITRQAVHDA"
     gene            complement(328..>1060)
                     /locus_tag="PKH07_20440"
     CDS             complement(328..>1060)
                     /locus_tag="PKH07_20440"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNT37374.1"
                     /translation="KWKYCHGADHYIAISNASARTVLAHGVTEDCITVVPSCYASEPD
                     MRLKGTNLLKAQLHLPDQMRVIAAIGGLSRIKGHEILLQAYAKITETHDDSVLVLAGD
                     GPEGEKLRAQIEQLSLSKRVFLLGFVKDLTPVYSDTDLFVLPSFFEGLNTSLLQAMAW
                     GIPCVATNVGGVPEAITDGVEGVLVPPGDADALARAIAGVWSNISTTTEMAQAARQKA
                     VAHFSKQQMVRGTESVYRDVLSKAY"
BASE COUNT          260 a          265 c          277 g          258 t
ORIGIN      
        1 cagcatcgtg taccgcctga cgagtgatgt gatgcatttc tgcgatctcg gacaatgaca
       61 gatcctcatc atagtgaagc gcaagaaaga ccttctgctt gtcgggcagc aactgcccgt
      121 aaatgtccaa gagcttcgac cacttcagtc gcgactctac ccgttctggg tcaaggagta
      181 agtcttcgtt catagcactc tttacgaatt ttgtgcctgt aaaggcaaaa cgctttacag
      241 atgagagcac aaatcagaga actttgtcaa ggtctgtttt ggataattgg agaagtgagg
      301 cgagtgcaga tggtgatttg ccatctccta ataggcttta ctgaggacgt cacgatagac
      361 agattcggta cctcgaacca tctgttgctt cgagaaatgg gcaacggctt tctgtcgtgc
      421 tgcctgcgcc atctcggtcg tcgtcgagat gtttgaccaa acaccggcta tcgctctcgc
      481 aagagcatcg gcatcccctg gtgggacaag aaccccttcg acaccatccg tgattgcttc
      541 cggaacacca cccacgttgg tagcgacaca ggggattccc catgccattg cctggagcag
      601 cgatgtgttc aggccttcaa aaaaagaagg gaggacaaag agatcggtat ctgaatagac
      661 cggagtgaga tctttcacaa aaccgagaag gaagactctt ttcgaaagag agagttgctc
      721 aatctgtgct ctgagcttct ctccctctgg accgtctcca gcaaggacga ggacagaatc
      781 atcatgtgtc tcagtgatct ttgcatacgc ctgcaggaga atctcatgac ccttgattcg
      841 ggacagccct ccgatggcgg ctatgactcg catttggtct gggagatgca gctgggcttt
      901 gagaaggtta gtgcctttga gtctcatgtc aggctcactg gcgtagcaac tgggtacaac
      961 cgtgatgcaa tcctcagtga cgccgtgagc caacacagtt cgggcagaag cattcgatat
     1021 ggcgatatag tgatcagccc cgtggcagta tttccatttc
//
LOCUS       DAOQUC010001628         1060 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_208660_length_1060_cov_2.188060, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001628 DAOQUC010000000
VERSION     DAOQUC010001628.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1060
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_208660_length_1060_cov_2.188060"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..451)
                     /locus_tag="PKH07_20445"
     CDS             complement(<1..451)
                     /locus_tag="PKH07_20445"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNT37375.1"
                     /translation="MQENLTIVHVDCGKTWGGGQAQVFMLLEGLRKIGHRNILVCRED
                     CPLAKRASEAGVASVAKARFGTLTMPRVVRILDKVTRDEGAHVLHLHDSRAHSIGALF
                     AAQTPERPPIVVSRRVAFDGRPTILSKWKYRHGADHYIAISNASARTV"
     gene            complement(519..>1060)
                     /locus_tag="PKH07_20450"
     CDS             complement(519..>1060)
                     /locus_tag="PKH07_20450"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNT37376.1"
                     /translation="RDIRQVQLNSLRTQIGLVPQSVILFNDTIRSNIAYGCDNARQED
                     VEKAARLANAHDFITGFPDGYDTMVGERGAQLSGGQAQRISIARALFKDPPILIFDEA
                     TSSLDSESELLIRKAMDNLILNRTTFIIAHRLSTVLHADQIIVLDKAKVAGIGKHEQL
                     LESNPIYRRLYELQFKPER"
BASE COUNT          236 a          269 c          287 g          268 t
ORIGIN      
        1 acacagttcg ggcagaagca ttcgatatgg cgatatagtg atcagccccg tggcggtatt
       61 tccatttcga gaggatcgtt ggcctgccgt caaaggcgac ccttcgggag acgacgatag
      121 gtggacgttc aggtgtctgg gcggcgaaaa gtgctcctat cgaatgggct ctggaatcgt
      181 gaagatgcag aacgtgagct ccttcgtccc gggtcacttt gtcaagaatg cgaacaacgc
      241 gaggcatggt gagcgttcca aaacgtgcct ttgcgacgga agcgacgcct gcttccgaag
      301 cgcgttttgc caaggggcag tcctctcgac agacaaggat gtttcgatga cctatcttac
      361 gaagaccctc aagaagcatg aaaacctgag cctgtccgcc cccccaggtc ttgccgcagt
      421 caacatgaac tatggtcaga ttctcctgca ttcaatgctc cttggtcgtc tctgaggttc
      481 tgatctcatc acctgtctac tggaacagtg tcttgctcct aacgctcagg tttgaactgg
      541 agctcgtaga gccttctgta gatagggttg gactcaagta gttgctcgtg tttcccgata
      601 cctgccacct ttgccttgtc caacacaatg atctggtcag cgtgaagcac tgtggagaga
      661 cggtgcgcaa tgataaaagt ggttctattc agtatcaggt tatccatggc ttttcggatc
      721 aacaactcgg attccgagtc gagagacgag gtggcctcat cgaatatgag gatgggcgga
      781 tctttgaaca aggctctggc gatcgagatg cgctgcgcct gaccgcccga aagttgggct
      841 cctctttcgc ctaccatcgt gtcgtatcca tccggaaatc ccgttatgaa atcgtgagca
      901 ttggctagcc gagcggcctt ttcaacatct tcctgtcttg cattgtcaca tccgtaggca
      961 atgttacttc gaatagtgtc gttgaaaaga atcacgcttt gaggcacaag gccgatctgt
     1021 gtgcgcagag aattgagttg cacttgtcgg atatccctgc
//
LOCUS       DAOQUC010001629         1059 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_208861_length_1059_cov_2.975100, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001629 DAOQUC010000000
VERSION     DAOQUC010001629.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1059
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_208861_length_1059_cov_2.975100"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="PKH07_20455"
     CDS             <1..364
                     /locus_tag="PKH07_20455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37377.1"
                     /translation="EFVHAVSWLKEAGFAASEIGAYVFVGYPGQTPEEAARACLFAHR
                     QGILVKQTQYAPTPQSAIWERGFPDFRFDPGEDPLLQNCTLMPWRSDTFPASVYFRLR
                     ERINRWNALAQRGCRCDE"
     gene            516..>1059
                     /locus_tag="PKH07_20460"
     CDS             516..>1059
                     /locus_tag="PKH07_20460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNT37378.1"
                     /translation="MANVEFRNVTKIFPGNVLAVDKFSLEIKDREFIVLVGPSGCGKS
                     TTLRMVAGLEEISEGEIYIDGRLVNDVPPKDRDIAMVFQNYALYPHMTVYNNMAFGLK
                     LRKYPKDEIRQRVQEAAEILGITELLNRKPKALSGGQRQRVAVGRAIVRKPKVFLFDE
                     PLSNLDAKLRVQMRAEIARLH"
BASE COUNT          248 a          259 c          292 g          260 t
ORIGIN      
        1 ggagtttgtc catgcggtgt cctggctcaa agaggctggt tttgcggcat cggagattgg
       61 ggcctacgtc tttgtggggt atccagggca gactccggaa gaggcggcac gtgcctgcct
      121 ttttgcacat aggcagggaa tccttgtcaa gcagacacaa tatgcaccca cgcctcaaag
      181 cgcgatctgg gaacgcggat ttcctgattt tcgttttgat cccggagagg atccactgct
      241 gcagaactgc actctgatgc cttggagatc ggacacattt ccagcctcgg tgtacttccg
      301 attgcgtgag agaatcaacc gctggaatgc tctcgcgcaa agggggtgcc gatgcgatga
      361 gtgagcaagc tctgccgacc gatattgtcg tggggctttt ggatcgctct gacagaaaac
      421 caggatcgtt ccgttcaacg ttgaatcctg ttgttttttg tgtatacttc tttggtactt
      481 tgcagggcag aatgatgaac ctcgggagag agtagatggc gaatgttgaa tttcgaaatg
      541 tgacgaagat attccccggt aatgttctcg cggtagacaa attcagtctg gagatcaaag
      601 accgggaatt cattgttctg gtaggccctt ccggatgcgg caaatccacc acacttcgga
      661 tggtcgcagg tctcgaagag atttctgagg gggagattta cattgacggc cgccttgtca
      721 acgacgttcc tcccaaagac cgagatatcg ccatggtgtt tcagaactac gccctctatc
      781 cacacatgac cgtgtacaac aacatggcgt ttggtctgaa acttcgcaaa taccccaaag
      841 acgagattag acagcgggtt caggaagcgg ctgaaattct tggaattact gaactgttga
      901 accgaaaacc gaaagctctt tctggggggc agcgtcagcg tgtggccgtc ggacgagcca
      961 ttgtcagaaa gccgaaagtc tttcttttcg acgagccact ctcaaacctc gatgccaaac
     1021 tccgagttca gatgcgcgcc gagatcgctc gtcttcaca
//
LOCUS       DAOQUC010001630         1059 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_208911_length_1059_cov_2.603586, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001630 DAOQUC010000000
VERSION     DAOQUC010001630.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1059
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_208911_length_1059_cov_2.603586"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="PKH07_20465"
     CDS             complement(<1..186)
                     /locus_tag="PKH07_20465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37379.1"
                     /translation="MSQAETISLLDDRLFDRGMQVTATSVFERPLYGKTVLARDATGT
                     PRWNLCQWASRFSLADAT"
     gene            complement(365..>1059)
                     /locus_tag="PKH07_20470"
     CDS             complement(365..>1059)
                     /locus_tag="PKH07_20470"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HNT37380.1"
                     /translation="VFLSCVMPVSAESEKVVPDTPCFVPLFDGVSLDGWKVSEGAKDH
                     WTVVEQRIVHDGQRSPGGDPNLWTEKAYRDFILKVQWRTVREPDMREVAVILPDGTYD
                     LDEEGKQKQVSVAFSVDSGIYLRGSSKSQINIWQWPIGSGEVYGYRTDPEMSPEVVRG
                     VTPKVCADKPLGEWNDFTIVMIGDRLSVRLNGQIVLENAQLPGVPQEGPIALQHHGDP
                     IEFKDLFIFEIE"
BASE COUNT          249 a          324 c          254 g          232 t
ORIGIN      
        1 cgtagcatcg gccaatgaga agcggctcgc ccactgacag aggttccagc gaggagtgcc
       61 cgttgcatcc cgcgccaaga ctgtcttccc gtacaacggt cgttcaaaga cgctcgtcgc
      121 cgtgacctgc atgcctctgt caaataagcg atcatccagc agtgagattg tttctgcttg
      181 actcattctg ctcccccaga ttacagacaa gaacacgagc atccagcgac atcctgctgc
      241 tccactgaca acgctccttc ttctgaaacg gaattcactg gcatgcatcc gtgcctcatt
      301 gctcctgctc gctgagcctc gaacgcagtc tcaggcaagg cgctaaggct taggcaaagc
      361 ccgcctattc aatctcaaag atgaagagat ccttgaactc gattgggtcg ccatggtgtt
      421 gaagcgcgat ggggccctcc tggggaactc cgggaagttg tgcattctcc agaacgatct
      481 gaccattgag tcttacggac aaccggtcgc cgatcatgac gatggtgaag tcgttccatt
      541 cgcccagagg cttgtcagcg cagactttcg gtgtcacacc ccgaacaact tcaggcgaca
      601 tctcgggatc agttcgatag ccgtagacct caccgctacc gataggccat tgccagatat
      661 ttatctggct cttggaactg cccctgagat agatgccact gtccacggag aaagcaacag
      721 aaacctgctt ctgtttcccc tcctcatcca aatcgtaagt tccgtcgggg aggatgactg
      781 ccacttcacg catgtcaggc tctctgactg tccgccattg gactttaaga atgaaatccc
      841 gataggcctt ctccgtccag agattggggt cgcccccggg actgcgctga ccatcatgga
      901 cgatgcgttg ttcgaccaca gtccaatgat ccttggcccc ttcagaaacc ttccaaccgt
      961 caagactgac tccgtcaaac aaaggcacga aacacggtgt atccggaaca actttctctg
     1021 actccgcaga gaccggcatc acgcaactca ggaacacaa
//
LOCUS       DAOQUC010001631         1057 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_209484_length_1057_cov_3.991018, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001631 DAOQUC010000000
VERSION     DAOQUC010001631.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1057
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_209484_length_1057_cov_3.991018"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1041)
                     /locus_tag="PKH07_20475"
     CDS             complement(<1..1041)
                     /locus_tag="PKH07_20475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNT37381.1"
                     /translation="MEELTGVSEEDILGRGDFEYAIPFYGHRRPLLVDLVNLSLHDTR
                     ELYSSIRREGETLIAETYAPSLKGGCHLWCKATPLYDSTGQTIGAIESVRDVTDALTA
                     KEALQDSEEKYRTLVENVNDGIAINQAGKFIYFNEQFASMLGYTQDELLMKDYREIFT
                     QEGLDLLEKRQEQRNMGRVVPNRYETKMLRKDGTEIDVEANVKIINYKGESTTFAVCR
                     DVTQRKKFEAQIQQSQKMESLGVLAGGIAHDFNNLLVGILGNASLSLKEIPTHSPARR
                     CLEQIERSAQRAADLTNQLLAYSGRGQIALELVNLTELVEEMIGLLEMSISKQAVLHH
                     SFAREMPSVEGDA"
BASE COUNT          219 a          285 c          273 g          280 t
ORIGIN      
        1 cgcatcaccc tcgacggatg gcatctcacg cgcaaaactg tgatgcaaga cggcctgctt
       61 ggagatcgac atttccagaa ggccaatcat ctcttcgacc agttcggtca aattgaccaa
      121 ttccaaggcg atctggcctc ttcccgaata cgccagcaac tggttggtca gatcggcggc
      181 gcgttgcgca gaacgctcaa tctgttccaa acaacggcga gcaggggaat gcgttggtat
      241 ttccttcagg gagaggctgg catttccaag gatacccacc agaaggttgt tgaaatcgtg
      301 ggcgattcca ccagcgagca ctcccaggct ctccatcttc tgagactgct gaatctgcgc
      361 ctcgaacttc ttgcgctgtg tgacatcgcg acaaacggcg aacgttgtgg actctccctt
      421 gtagttgatg attttgacat tggcttcaac gtcgatctcg gtgccatcct tgcgcagcat
      481 cttggtctcg tatcggttcg gcacgactcg tcccatgttt ctttgttctt ggcgtttctc
      541 tagaagatca aggccttcct gagtgaatat ctcacggtag tctttcatca gaagttcgtc
      601 ttgtgtgtag cctaacatgg aggcgaactg ctcattgaag tagatgaact tccctgcctg
      661 gttgatcgcg ataccatcgt tgacgttctc cacgagggtt ctgtatttct cttcggaatc
      721 ctggagtgct tcttttgcgg tcaacgcgtc tgtgacatcc cttactgatt ctatagcgcc
      781 gatcgtctgg cctgttgagt catagagggg tgttgctttg caccaaagat gacatccgcc
      841 tttcagagac ggagcatagg tctcggcgat caaggtttcc ccctcgcggc ggatcgagga
      901 atagagttcc cttgtgtcat ggagcgagag attcaccaag tcaacaagca gcggccttct
      961 gtggccatag aaggggatcg cgtactcaaa atcacctctt cccaagatgt cttcttccga
     1021 gacgcctgtg agctcctcca tggcgtgatt ccaggcg
//
LOCUS       DAOQUC010001632         1056 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_209965_length_1056_cov_2.346653, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001632 DAOQUC010000000
VERSION     DAOQUC010001632.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1056
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_209965_length_1056_cov_2.346653"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="PKH07_20480"
     CDS             <1..181
                     /locus_tag="PKH07_20480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37382.1"
                     /translation="LWQVMKASDGRKRNCRLDIMAVVVCQSTRTDGVMCTPASTYGDL
                     ELQAVLFGSILHGHT"
     gene            168..>1056
                     /locus_tag="PKH07_20485"
     CDS             168..>1056
                     /locus_tag="PKH07_20485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37383.1"
                     /translation="MDTLEYWEERLAVLPTTIDCSEMHILGEDRQAPVFAGPGHIDIK
                     SENDIDFTMYGTSTDGSDAIQRLCRAYENPYQVADQFVLEATDYRGTQWNCGWTHPEY
                     KDSTGSSYLLMGTVNSLVTLARGHAVSKTSSIELIFPPDLWMPMDKTMVSVSSIDGAE
                     VAREYRPGQQAVNVLDSEIKFFYRPPSNSLWLTAATSDTLVHPYAESWVCEPLRILLG
                     QLVYPRLVARNFGDGTAHVWLRRSARLFSGLGIASLLSDDPDMRRCFWELYARLLQLI
                     AKPKIASSLASLELHPITRF"
BASE COUNT          234 a          274 c          291 g          257 t
ORIGIN      
        1 actttggcag gtcatgaagg cctcggatgg gcgcaagagg aattgccgtc tggatatcat
       61 ggctgtcgtg gtgtgtcaaa gcacaagaac agatggcgtg atgtgcactc ctgcgtcgac
      121 ttacggtgat ctggaattgc aggcagtact ctttggaagt attcttcatg gacacacttg
      181 agtattggga agaacgtctg gctgtgctgc caactaccat tgattgttcg gagatgcaca
      241 tcttgggcga agaccggcag gcacctgtct ttgctggccc tggccacatc gatatcaaaa
      301 gcgaaaacga cattgacttc acaatgtatg gaacatctac ggacggctca gatgccatcc
      361 agcgactctg tcgtgcatac gagaacccgt atcaggttgc tgaccaattc gttctggagg
      421 caacggacta tcgaggaacc caatggaact gtggttggac gcacccggaa tacaaggaca
      481 gtacgggttc cagttatctg ctcatgggca cagtcaattc gctggtcacc ctcgcacgag
      541 ggcacgcggt atcgaagaca tcgagtatag aactcatctt cccgcctgat ctctggatgc
      601 cgatggataa gacgatggtg agtgtttcgt caatagacgg tgcggaggtc gcacgcgaat
      661 atcgccccgg ccaacaggca gtcaacgtac ttgattctga gatcaagttc ttctatcgac
      721 ctccgagtaa ctcgctctgg ttgactgccg caacaagcga cacgctcgta cacccttatg
      781 cagaaagttg ggtctgtgaa cctctgcgta ttctgcttgg acagcttgtc taccctcgcc
      841 ttgttgcgcg gaacttcggc gatggaacgg cacatgtgtg gcttcgtcgg tcggctcgct
      901 tgttctccgg gttgggtatt gcctcgctgc tcagcgatga tcccgacatg aggcgctgct
      961 tttgggagct ctatgcgaga ttactccagt tgattgccaa acccaagatt gccagcagcc
     1021 ttgcgagcct ggaattgcat ccgatcaccc gcttct
//
LOCUS       DAOQUC010001633         1054 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_210549_length_1054_cov_2.756757, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001633 DAOQUC010000000
VERSION     DAOQUC010001633.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1054
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_210549_length_1054_cov_2.756757"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1054)
                     /locus_tag="PKH07_20490"
     CDS             complement(<1..>1054)
                     /locus_tag="PKH07_20490"
                     /inference="COORDINATES: protein motif:HMM:NF020121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="beta-galactosidase trimerization
                     domain-containing protein"
                     /protein_id="HNT37384.1"
                     /translation="GYCRIGAPERWTAFEETLRLVRQMPEVRLPAETKTMVLVEPSSH
                     AALPITEMGSHAEFPTCFDFLGSHAQSWFKFVHGGQLERGEVSLSDYRIIYLPMAKYF
                     KSSLAGQILEFANNGGTVICADPEVFSFASDKDKLPPDARQILGLEVEPVTVTRSRMT
                     IVDNTFFPSLPTSESFMLSKEVQSYRGKGDGSWKTLATFEDGTPAIARKTLGKGFWIQ
                     FAFQPFQPKLLETPELRALFKSLQQYLGEPTDLDIWRFRFPTPENLPQPPEGKCLTGN
                     AVFWKQNEPVSVGGDGVKGKTVWTKNPVGVSQSEIAFETSRLFDRRKAVQAPNDKTHA
                     AACQNWAYQWNGTEAFD"
BASE COUNT          250 a          297 c          268 g          239 t
ORIGIN      
        1 atcaaatgcc tccgtgccgt tccactggta agcccagttt tgacatgccg ccgcatgcgt
       61 cttgtcattg ggagcctgta ccgctttgcg tcggtcgaaa agtctgctgg tctcaaatgc
      121 aatctctgac tgagaaacac caacaggatt ctttgtccag accgtcttcc ctttgacacc
      181 atctcctcca acggaaactg gctcgttttg cttccagaag acggcattgc ccgtcagaca
      241 cttgccctct ggcggctgcg gaagattctc cggcgtcggg aaacgaaacc gccagatgtc
      301 caaatcggta ggctccccaa ggtactgctg aagagacttg aacagcgcac gcagttcggg
      361 agtctcgaga agttttggct ggaatggctg aaaggcgaac tgaatccaga agcccttgcc
      421 aagcgtcttc ctcgcaattg caggagtgcc gtcttcaaac gttgccaatg tcttccaact
      481 cccgtcaccc ttccctcggt atgactgcac ttccttggac agcatgaatg attcgctggt
      541 cggcagtgac ggaaagaagg tgttgtcaac gatggtcatt ctgcttcgag tcacggtcac
      601 aggctccact tccagcccca gtatctggcg tgcatcagga ggaagtttgt ccttgtccga
      661 agcaaacgaa aacacctcag gatcagcaca aatgaccgtt ccgccattgt ttgcgaattc
      721 cagtatctga ccagcgagag atgacttgaa gtattttgcc ataggaaggt agatgattct
      781 gtaatcactc agcgaaactt cgcctcgctc cagctgaccg ccatgcacga acttgaacca
      841 ggactgggca tgagagccaa gaaagtcaaa gcaggtgggg aactccgcat gggatcccat
      901 ctccgtgatt ggcaatgcag cgtgactgga cggctcaacg agaaccatgg tctttgtttc
      961 ggcaggcagt cgtacttctg gcatctgacg aactaaccga agagtctcct caaaagccgt
     1021 ccaacgctcc ggcgcaccaa tgcggcagta accg
//
LOCUS       DAOQUC010001634         1051 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_211635_length_1051_cov_2.355422, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001634 DAOQUC010000000
VERSION     DAOQUC010001634.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1051
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_211635_length_1051_cov_2.355422"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            121..552
                     /locus_tag="PKH07_20495"
     CDS             121..552
                     /locus_tag="PKH07_20495"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HNT37385.1"
                     /translation="MRIYMDNCCFNRPFDDQTLLRIRIETEAKLYVQELLRQKAIELA
                     WSYVLDFENDANPFKERREAVESWRTLSVVDVDERPEVVQLADRYTLFGLHPRDSLHI
                     ACAVVADCDAFLTTDRKLLAKHHQIAEIRLIDPPAFLREMT"
     gene            552..788
                     /locus_tag="PKH07_20500"
     CDS             552..788
                     /locus_tag="PKH07_20500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002708881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37386.1"
                     /translation="MKTDTEIRTLGFRALVKAMGTLEAERFVTMLLREPFDYTEWRQT
                     LWDGMDVETISRAAMSARKSKEEESPGANQAAEP"
BASE COUNT          270 a          259 c          287 g          235 t
ORIGIN      
        1 tttgcacata ggggacatta ctactttgct gcgacaaggc aagcacgggg attgacttga
       61 tcagcgtatc ttgcgagaat cgcgcagtag gtatagattg ccgtgtgcaa ggaagaatga
      121 atgcgaatct acatggacaa ctgttgcttc aaccggccgt ttgacgacca gactcttttg
      181 cgtattcgca ttgagaccga agcaaaactg tatgttcagg agttgttaag gcaaaaagca
      241 atcgaactgg cttggtccta tgttcttgac ttcgaaaacg acgccaaccc cttcaaggag
      301 cggcgtgaag ccgtcgagtc gtggcgaacc ctgtccgtcg ttgatgtgga tgagagaccg
      361 gaggtcgtcc aactcgcaga tcgctacaca cttttcggac ttcatccgcg cgattccttg
      421 catatcgctt gcgctgttgt tgcagactgt gatgctttct tgaccacgga tcgaaaactt
      481 cttgccaagc atcaccagat cgccgagatt cggcttatcg atccaccggc ttttctgagg
      541 gagatgacat gatgaaaacc gataccgaga tcaggactct gggctttcgc gcactggtaa
      601 aggctatggg aactctcgaa gccgagagat tcgtgacgat gttgctccga gaaccgtttg
      661 actatacgga atggcgccag acactttggg acggaatgga cgtggaaacg atcagccgtg
      721 cagcgatgtc agcgcgaaag tcaaaggaag aagaatcgcc tggagcgaac caggccgctg
      781 agccgtaacg ttctccatcg agatctgaac atgcgtaact cagaatgaac tccaatgacc
      841 acacagggag ccatgccccg ttccagactt caggttcgcg aagcggctct gaggactgtt
      901 tttctggcga gtaactcggc ttcccccgaa atctgaaacc gttcttgaca tccgtacacg
      961 ggctactact cagcgcggga catgagtcgt cgcgaacgca aggagtatga aaatgcggaa
     1021 aaaagcgctg aagaagatac cacggtttga g
//
LOCUS       DAOQUC010001635         1049 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_212237_length_1049_cov_2.965795, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001635 DAOQUC010000000
VERSION     DAOQUC010001635.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1049
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_212237_length_1049_cov_2.965795"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(45..>1049)
                     /locus_tag="PKH07_20505"
     CDS             complement(45..>1049)
                     /locus_tag="PKH07_20505"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HNT37387.1"
                     /translation="AIDKKASDLRDKVVFAVTQDKIQKLDIRYPEAHGVVVSGTESEK
                     WRIREPFDAVADWNDLNSFMGRISRLRIKEFVEESPSDLGAYGLVGDVTRLAVFEADR
                     AEPVTLLIGRYDSAQSAYYAKHDNKPNVFSVEAPFVNALPNTAADWRPDKPLPMWDYQ
                     ARTVRVERGDGTVSLELGKDESQNWILKEPIQSASAELDTNKVREFVNALQEYSIVRY
                     ATETLDPSVTGLGSPDYTVTVSGEVSGATKTYQLQIGNKVPDELLVYGKRSSDDEVFM
                     GSISILAQVHITHEDLVKQVTAPNFSESESESEDSEMEPEPGSLEVLPGVNLKQAEPE
                     "
BASE COUNT          204 a          285 c          293 g          267 t
ORIGIN      
        1 gaaggtaggg gcaggtttgt gccctgcccc tacgcggagt gtgatcattc gggttcggct
       61 tgcttcagat tgacgcccgg aaggacttcc aacgatccgg gctccggttc catttccgag
      121 tcttccgatt cagactctga ctctgagaag ttcggggcag tcacttgctt gaccagatct
      181 tcatgagtta tgtgaacctg tgcgaggatg gaaatgcttc ccatgaagac ttcatcatcc
      241 gacgagcgtt tgccgtaaac cagaagttca tcgggaactt tgttcccgat ttgaagttga
      301 tatgttttgg ttgcgccgct gacttcaccg ctgactgtga ccgtgtaatc gggcgagccc
      361 aaaccggtca cactcggatc gagtgtctcc gttgcatagc gaacaatgct gtattcctgg
      421 agggcattga cgaattcccg caccttgttg gtatccaact cagcacttgc agactgaatc
      481 ggttctttca gaatccagtt ctgggattcg tccttgccaa gttccagaga aaccgttccg
      541 tcgccacgct cgacccgaac tgttctcgcc tggtaatccc acatcgggag cggcttgtcc
      601 ggtcgccagt ccgcggctgt gttcggcagc gcgttgacaa agggtgcttc gactgagaag
      661 acgttgggct tgttgtcatg tttggcgtag taggcactct gggcgctgtc gtaacggcct
      721 atcagcagtg tcacgggttc agcgcggtcg gcttcgaaca cggccagccg tgtcacatcg
      781 ccaaccaggc cgtatgcgcc aaggtctgag ggtgattcct cgacaaactc cttgatacgc
      841 aggcgggaga tgcgtcccat gaaactgttg agatcgttcc agtctgcaac cgcatcgaag
      901 ggttcccgta tacgccattt ttccgattcc gttcctgaaa ccacgactcc atgtgcttcg
      961 ggatagcgga tgtcgagttt ctggatcttg tcctgggtca cagcgaagac gaccttgtca
     1021 cggagatccg aggccttctt gtcaatggc
//
LOCUS       DAOQUC010001636         1048 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_212754_length_1048_cov_1.988922, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001636 DAOQUC010000000
VERSION     DAOQUC010001636.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1048
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_212754_length_1048_cov_1.988922"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            35..>1048
                     /locus_tag="PKH07_20510"
     CDS             35..>1048
                     /locus_tag="PKH07_20510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37388.1"
                     /translation="MTGGCEESKQCIIDQIVNGINEMGAQMGFSAIQSSSDTFRIESQ
                     SLPFSKCLQSDYEMDLPYGMGLPLTLGCDVNNMCSAQIGDETQPLWRGYGFHVISETL
                     PTPTFTPTSTPTMKPVNPDAFHVLTSGQVVSFESISDASPDILDLSPWQNQDAIFSDI
                     TAYQDGRIGLSDSSTGHGFAFRDVFDSSPIVHDLPGMYGVSDLDDTDENTVIYLDPGM
                     PKVAVLDVPGQWIHELYLTIGYPASPRDIAYLGEDRFAVLVEAGDIGANPTGRIDIFD
                     IPVGQIYQVTPSLSLPLAFPASGCRMDASPDGSLFVLNPSANSLFAYGYPYDVTFTKV
                     IPQA"
BASE COUNT          237 a          272 c          276 g          263 t
ORIGIN      
        1 gctcttgtct gttgaggtct ggtgtgaaaa ctgcatgacc ggcggatgtg aggaatcaaa
       61 gcagtgcatt attgaccaga tcgtcaatgg catcaatgag atgggtgccc aaatgggctt
      121 cagcgccatt caaagcagca gcgacacgtt ccgcattgaa agtcagtctc tgccgttcag
      181 caagtgtctg cagagcgact acgagatgga cttgccctac ggcatggggc tgccactgac
      241 cttgggatgc gacgtcaaca atatgtgcag tgctcagatt ggtgatgaga cccaacctct
      301 gtggcgcggc tatggtttcc atgtcatttc tgagacgctg ccaactccga cgttcactcc
      361 aaccagtact ccgacaatga agccggtaaa tcctgacgct ttccatgtac tcaccagtgg
      421 tcaggtggtc tcattcgaga gtatctcaga cgcaagtccc gacatactcg atctgagtcc
      481 ctggcagaac caggacgcca tcttcagtga catcacggcc tatcaggatg ggaggatcgg
      541 attgtcagac agttcgactg gacatggctt tgccttcaga gatgtattcg attccagtcc
      601 gatcgtccat gatctgcctg gaatgtatgg tgtctcagac cttgacgaca cggacgagaa
      661 cactgtcatc tatctggatc ctgggatgcc caaagtggca gttctggatg ttccaggaca
      721 gtggattcat gagttgtact tgaccatcgg atatcctgct tcccctcggg atattgcata
      781 tcttggcgag gatcgctttg cggtgctcgt cgaggcaggc gacatcgggg caaacccaac
      841 aggacgcata gacatctttg acattcctgt gggtcagata taccaagtga caccgtctct
      901 gagtctgccg ttggctttcc ctgcttccgg atgtcgaatg gatgcatcgc cagatggatc
      961 actgtttgtc ctgaatccca gtgcgaacag tctttttgcc tacggctatc cgtatgacgt
     1021 cactttcaca aaggtcattc cacaagcg
//
LOCUS       DAOQUC010001637         1046 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_213311_length_1046_cov_2.709384, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001637 DAOQUC010000000
VERSION     DAOQUC010001637.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1046
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_213311_length_1046_cov_2.709384"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..426)
                     /locus_tag="PKH07_20515"
     CDS             complement(<1..426)
                     /locus_tag="PKH07_20515"
                     /inference="COORDINATES: protein motif:HMM:NF018155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase
                     (2,3-diphosphoglycerate-independent)"
                     /protein_id="HNT37389.1"
                     /translation="MAKAVLAAYARGDEDEAMEPLALLGADGLPTGRILPGEPVIFYD
                     IRGEREIELTQSFTDPAFSHFVTSGGTSEFVTMIQYDPSLDVRVAFPPEEHLAQTLSA
                     TVSQAGLRQLKVAESEKAVHLSYFFNGKSQEAEPGEERIT"
     gene            complement(547..>1046)
                     /gene="nuoB"
                     /locus_tag="PKH07_20520"
     CDS             complement(547..>1046)
                     /gene="nuoB"
                     /locus_tag="PKH07_20520"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015774789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HNT37390.1"
                     /translation="TREYRLARTSREDLVVVNAQRERHAAIEDLKQRLFRRSLRLRQV
                     SAGGCNACEADSNVLGTLAWDIGRFGMQFVASPRHADGLLVTGPVSENMKLALLKTYE
                     AVPDPKIVIATGSCAISGGPYIDHDEVCNGVTDLLPVDLFIPGCPPHPLTILDGLLFF
                     LGLVP"
BASE COUNT          238 a          275 c          294 g          239 t
ORIGIN      
        1 ggtgatccgc tcttctccag gttccgcttc ctggcttttt ccattgaaga agtaagagag
       61 gtgaacggcc ttttctgact cggccacttt tagttgacgt agaccagcct gcgatactgt
      121 tgctgagaga gtttgtgcga gatgctcttc aggtggaaag gccactcgaa catccagaga
      181 cgggtcgtat tggatcatcg tcacaaactc ggatgttcca ccgctggtca caaaatgcga
      241 gaaggctgga tccgtgaaac tctgcgtgag ttcgatctcc cgctcgccac ggatgtcgta
      301 aaagatgacc ggttctccag ggagaatacg ccctgttggc aaaccgtctg ctcccaggag
      361 tgccagtggc tccatcgcct catcttcgtc acctcgggca tacgccgcaa ggactgcttt
      421 tgccatcgga taatcagttg gtttgggttc tgtcataatg agggggagaa tagaccagac
      481 atcgcctcaa cgtcaagaaa gggattcagc gcggattctg ggtcagagcg gagaaaccgt
      541 cgagactcag ggaaccagac caagaaagaa caacaagccg tcgagaattg tcagaggatg
      601 aggcggacat cccgggatga acaaatccac aggcaggaga tcagttactc cgttgcatac
      661 ttcgtcgtgg tcaatatagg gtccaccgga gatggcgcaa gagcctgtgg cgatgacgat
      721 cttgggatca ggaactgcct cataggtctt cagcaaggcc agtttcatgt tctctgagac
      781 cggtccggta acgagaagtc catcagcgtg tcgtggcgac gcgacaaact gcattccgaa
      841 acgaccgata tcccacgcca gggttccgag gacatttgaa tccgcctcgc aggcattgca
      901 gccccctgcg ctgacctgtc ggagtctcag cgatctcctg aagagtctct gtttcaaatc
      961 ttctatcgcg gcatggcgct cacgttgtgc gttgacgacc acgaggtctt ctctgcttgt
     1021 tcttgcgagt cggtactcgc gggtga
//
LOCUS       DAOQUC010001638         1046 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_213419_length_1046_cov_2.175580, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001638 DAOQUC010000000
VERSION     DAOQUC010001638.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1046
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_213419_length_1046_cov_2.175580"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..907
                     /locus_tag="PKH07_20525"
     CDS             <1..907
                     /locus_tag="PKH07_20525"
                     /inference="COORDINATES: protein motif:HMM:NF012618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37391.1"
                     /translation="LAGGGFVGNFRTLFYDLVRDRFLDTEARGFPVAFTEDKSELVTA
                     DGNTVSGEWMVSRWAPTDSKPLWEARNPGIGGSPSCFPVACEDRKLAAQGFYGGDVAV
                     INYAEGRMLKSWRAHDSGVSTLAFSPDGQLVVTGESTPTPDTCSVVVWSCSDLESIEP
                     RHSLERHQQPVHAAIFTRDGRLLATGDSDGRIILWDVNTGGFRCELYGHKQDIYTLAF
                     SREGKTLASASHDGTVKLWHVATGRELITFALESCGTSVAFSPDGNTLVATSGPNPYE
                     PDRLYIWRVSSLKEIEGAMEIAVQE"
BASE COUNT          227 a          257 c          334 g          228 t
ORIGIN      
        1 tctggcaggg ggcggttttg tcgggaactt caggacgctc ttctacgatc tcgtgcgtga
       61 tcgtttcttg gacacggagg caagaggttt tcccgttgcg ttcactgagg acaagagcga
      121 gcttgtcact gccgacggga ataccgtctc cggggagtgg atggtgagta ggtgggcccc
      181 gactgattcc aagcccctct gggaagcgag aaacccaggc attggcgggt ccccatcatg
      241 cttcccggtc gcttgcgagg accgcaagct tgcggctcag ggcttctatg gcggggatgt
      301 tgccgtgatc aactacgcag aaggcagaat gctgaagagt tggagggcac acgatagcgg
      361 agtcagcacg ctcgcgttct cgccagatgg ccagttggtt gtgaccggtg agtctacgcc
      421 cacgccggac acatgttcgg tggtggtttg gtcgtgctcc gatctggaga gcattgaacc
      481 gcgacatagc cttgagagac accagcaacc tgtgcacgcg gcgatcttca cgcgtgacgg
      541 gagactcttg gctacggggg attcagatgg ccgaatcata ctctgggacg tgaacacagg
      601 tggatttcgc tgtgaactat atggtcacaa acaagacatc tataccctcg ccttttcccg
      661 tgaagggaag accttggcgt cggcaagtca tgatggaacc gtgaagcttt ggcacgtggc
      721 gacgggacga gagctgatca cctttgctct ggagtcttgt ggaacatctg tcgcattctc
      781 tcccgatggg aacactctcg tggcaacgag cggtccgaat ccttatgagc ctgaccgact
      841 atacatctgg cgagtttctt cgttgaagga gatcgaagga gcgatggaaa tcgcggtcca
      901 agagtgatgc ggttcgtttg aaatgaatca tcaggtgaca ggtaggatag aatcacaatg
      961 gcatcgaaga agaatgccga agagatcttc aacatggcgg tcgagctatc ggatgccgcg
     1021 gagcgggcgg cattcctgga cgaggc
//
LOCUS       DAOQUC010001639         1045 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_213708_length_1045_cov_2.402020, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001639 DAOQUC010000000
VERSION     DAOQUC010001639.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1045
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_213708_length_1045_cov_2.402020"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1045)
                     /locus_tag="PKH07_20530"
     CDS             complement(<1..>1045)
                     /locus_tag="PKH07_20530"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HNT37392.1"
                     /translation="PIICRHSGVYVKGIGDAIMARFAKPVEAVEAACAMLRSLVAHNT
                     ACEDRQQLHVRIGIATGRALIEPEDVNGHVVNVSARLCKQAPQDGILVCEETHASLDS
                     YLSCRCVLEAPLAVRGFETDTLRAYRVIWRDDVPVRVPSFDEEFLVLEVTSDASKLRL
                     SLHSGGKGQGTVSRYEELKYDLTVVQAACARIQHALMQATLNASSAQLEMLQDAGKTL
                     FDVLLTVDLKKRLSEATAEHLVLQIQDSLVSIPWELLHDGTDFLCRRFCVGRRVQSRE
                     QVAVAIRPPVLKPHVSILSCSWPDLPAAEREAERVYERLVSHTRVTVAMRKSATRDYL
                     RDQLRHSEIVHYCG"
BASE COUNT          250 a          309 c          265 g          221 t
ORIGIN      
        1 gaccgcagta gtgaacaatc tcggaatgcc tcagttgatc tcggagataa tcgcgggttg
       61 ctgatttccg cattgccacc gtgacgcgcg tatgcgaaac cagcctttcg tagactcgtt
      121 ccgcttctcg ctctgcggcg ggcaaatctg gccaagagca acttaggatg ctgacgtgag
      181 gcttcagaac cgggggacgg atggcaaccg cgacctgctc gcgactttga acacgacgcc
      241 caacgcagaa acgtcggcag aggaaatccg ttccgtcatg gagcagttcc cacggaatac
      301 tcaccagtga atcctgaatc tggagcacca gatgctccgc cgttgcttca ctcagacgtt
      361 tcttcaagtc cacagtcaga agcacgtcaa acaaggtctt gccagcatcc tgaagcatct
      421 ccaactgagc agagctggcg ttcaaagttg cctgcatcaa ggcgtgttga attcgcgcgc
      481 aggctgcctg aaccaccgtc agatcatact tgagttcctc gtatcggctt actgtgcctt
      541 gtcccttgcc ccctgaatga agggaaagcc tcaacttgga tgcatctgaa gtgacttcca
      601 aaacaagaaa ctcttcatca aatgaaggta ctctgacggg tacgtcatcc cgccagatca
      661 cccggtaagc gcgaagcgta tctgtctcaa agcctcgaac cgcgagaggc gcttccagaa
      721 cgcaccgaca tgagagatag gaatccagac tcgcatgggt ttcctcacag accagaattc
      781 cgtcctgagg tgcttgcttg cacaaacggg cgctgacgtt taccacatgc ccattgacat
      841 cttccggttc gatgagagcg cgacccgtag cgatcccaat tcgaacatgc aactgctgtc
      901 tatcttcaca agcggtgttg tgtgcgacaa gacttcgcaa catcgcgcag gccgcttcca
      961 ccgcctcaac gggcttggca aaccgtgcca taatcgcgtc gccaatgccc ttgacataga
     1021 cccctgaatg ccggcagatg atggg
//
LOCUS       DAOQUC010001640         1045 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_213720_length_1045_cov_2.311111, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001640 DAOQUC010000000
VERSION     DAOQUC010001640.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1045
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_213720_length_1045_cov_2.311111"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..594)
                     /locus_tag="PKH07_20535"
     CDS             complement(<1..594)
                     /locus_tag="PKH07_20535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37393.1"
                     /translation="MSVSETLDPSIQNLRISRCRFTIRAVDELRLCSLPGVQFSEGAG
                     RFLNHLWCSLPELAAEFDRGQACQFCILQRSCPYVRYWRSLGSDIRSHVVEAIGPAVV
                     AQGDAFEFDLLLFGETIDSLGAWIIALNDLGVHGIYRPGENEDRRRFSVLKVVERLPG
                     GCSSEIYDGDNSRMSALPVGASLQQWCESLPAVEGLIL"
     gene            complement(705..>1045)
                     /locus_tag="PKH07_20540"
     CDS             complement(705..>1045)
                     /locus_tag="PKH07_20540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37394.1"
                     /translation="IIGNVGTWILFRLGIRDARVLNEALGEQYDVARLANLPNYQTFV
                     RPMVHGKPLDPFFCLSLPPLPENRDNKSKALISKTLKTLGRPRVEVNDQILKTILNLS
                     EKKTERDNGL"
BASE COUNT          279 a          283 c          266 g          217 t
ORIGIN      
        1 cagaatgaga ccctccacag caggaagcga ctcacaccat tgctgcagag aggctccgac
       61 gggcagagcg ctcattcgag aattgtctcc atcatagatc tcgcttgagc atccgccggg
      121 caggcgttca accaccttaa gcacagagaa cctcctccga tcctcattct ctcccggtct
      181 gtagatgccg tgaacgccga gatcgttcaa ggcaatgatc caagcaccca ggctgtctat
      241 cgtctcccca aagaggagta gatcaaactc gaaggcatca ccctgagcaa caactgctgg
      301 accaattgct tcgacaacat gggaacggat atctgaaccc agcgagcgcc aatacctcac
      361 atacggacac gacctttgaa gaatgcagaa ctgacaagcc tgtcctcgat cgaattcagc
      421 tgccaactca ggaagagaac accagagatg attgagaaac cgccccgccc cttccgaaaa
      481 ttgaacaccg ggaagagaac acagccgcag ttcatccacc gcccgaatag tgaatcggca
      541 gcgtgatatc cgaaggttct gaattgaggg atcaagcgtt tccgatactg acaagagcat
      601 actccacgag ttcggtttgt ctccactacg cggaggaatg ctagattctc ggcaggcagg
      661 ctgtcaaatg gaggaaagca gggtgagaac aggcacactc ggtgtcacaa tccattgtct
      721 ctttcggtct tcttctcgga gagattgagg attgtcttga ggatctgatc gttgacttcg
      781 accctcggac gaccaagcgt cttaagcgtc tttgagataa gggctttgct cttgttgtct
      841 ctgttttccg gaagcggagg aagagagaga cagaagaaag gatcaagagg ctttccgtga
      901 accatgggcc gcacaaacgt ctgatagttg gggaggtttg ccagacgcgc aacatcgtat
      961 tgctcgccaa gcgcttcgtt gagcactctg gcgtcccgaa taccaagacg gaaaagtatc
     1021 cacgttccga cattcccgat aattg
//
LOCUS       DAOQUC010001641         1045 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_213725_length_1045_cov_2.306061, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001641 DAOQUC010000000
VERSION     DAOQUC010001641.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1045
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_213725_length_1045_cov_2.306061"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..482)
                     /locus_tag="PKH07_20545"
     CDS             complement(<1..482)
                     /locus_tag="PKH07_20545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37395.1"
                     /translation="MNPMAGAKRAEHVHSSCLQITAAHSQWSQRDPEHSRHLAAQTVF
                     LDITETLGTTFVPDLFAALERTPAYLETAWELFKDAVDLEVLDAQTKHIVALAITTNR
                     SGTYQIAAFPHAFRLSPVGSRRCETILSLIEMFQAFSRYLSDAVLVRHETNELGPGFA
                     "
     gene            complement(482..724)
                     /locus_tag="PKH07_20550"
     CDS             complement(482..724)
                     /locus_tag="PKH07_20550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37396.1"
                     /translation="MRRVDFYFHDGCLSQPSLLSLAKDIEAAYPTWTVTIHPLSADEV
                     NAMGFTILPTITINNVPTAFGTPCREWLLEAIRMCE"
BASE COUNT          241 a          285 c          294 g          225 t
ORIGIN      
        1 caagcgaatc ccggacccag ttcgttcgtc tcatggcgaa ccagcaccgc gtctgagagg
       61 tatcggctga acgcctgaaa catctcaatc agagacagaa tggtctcgca tctccttgaa
      121 cccacaggac tcagccgaaa ggcatgtggg aatgcggcaa tctggtacgt gccactccta
      181 ttcgtggtga tggccagtgc cacgatatgt ttggtttgag catcaaggac ctcaagatcg
      241 accgcatcct tgaagagttc ccatgctgtc tcgagatagg ccggtgttct ctccagtgcg
      301 gcaaacaagt cgggcacgaa cgtcgtgccc agggtctcgg taatgtccag aaagaccgtc
      361 tgcgcggcca aatggcgtga atgttcagga tcgcgttgcg accattgcga atgtgcggct
      421 gttatctgca aacatgagga gtggacgtgc tctgccctct tggctcctgc catcgggttc
      481 attactcaca catccgaatg gcctctaaca gccattctct gcatggtgtg ccgaaggcag
      541 tcggcacatt gttgatagtg atcgtcggca gaatcgtaaa gcccatcgcg ttcacttcat
      601 ccgctgagag cggatgaatc gtcacggtcc acgtgggata cgccgcctca atgtcctttg
      661 cgagtgagag aagcgagggc tgtgacaggc acccgtcatg gaaataaaag tcaactcgcc
      721 gcatacgcgc agcataggtc agccggtcca aaggaatctg taagctaggt tacaaggaag
      781 aaattgttgc tggaattcat gcaggcacca gcttcctccg ctggagcaca gtgcaccgga
      841 aaaccctaga ggggggagcc gggatcacgg aatgttcgat cagatgactg aattgccgtg
      901 gttcatccat gaccgctgtc ttgccgagac gctcacccgc aggatccgtt tcctccctgc
      961 tgcataaagc agggaggaga cgatggccac cagcaatcgc tcagtctatc cgcgcgttcg
     1021 gctgaaggtt tctccagagg atcgt
//
LOCUS       DAOQUC010001642         1044 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_213979_length_1044_cov_3.082912, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001642 DAOQUC010000000
VERSION     DAOQUC010001642.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1044
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_213979_length_1044_cov_3.082912"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            130..705
                     /locus_tag="PKH07_20555"
     CDS             130..705
                     /locus_tag="PKH07_20555"
                     /inference="COORDINATES: protein motif:HMM:NF025210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HNT37397.1"
                     /translation="MNYKVPVCVLSSFEEVPGTLVTEEAEDMERVMVRGVTSIPEAKV
                     SLVGVPDKPGVAAKIFGDIAARNINVDMIIQNVGSDDHNDISFTVSRSDLKAVIAIAN
                     DLVKEIHAQKVQSDDKVVKVSAVGVGMRSHSGVAGKMFSALANKGINIQLISTSEIKI
                     SCIIAEEYAELAVRALAEEFELENESKELEQ"
     gene            702..>1044
                     /locus_tag="PKH07_20560"
     CDS             702..>1044
                     /locus_tag="PKH07_20560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010868468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HNT37398.1"
                     /translation="MTRPRNVRLYDTTLRDGCQAEGVSFSVREKVMIAHKLDEFGIHY
                     IEGGWPGSNPKDIEFFQEIYKDPPKNAIITAFGSTRRAKVTADKDPNLCAIVESKVRA
                     AAIFGKCWVRHV"
BASE COUNT          266 a          244 c          294 g          240 t
ORIGIN      
        1 cgcgtctgaa gtacctttga tccgaggctt gccagctcca acatctcttc atatgagatc
       61 ctctccagca ttttggagct ggcaagcctc ggatcaaagg tacttcagac gcgatctgtc
      121 gagtttgcca tgaattacaa ggttcccgtc tgtgtgttga gcagctttga agaagttccg
      181 ggaacgctgg tcacggagga ggcagaggat atggaaagag tgatggtacg tggtgtcacc
      241 tcaattcctg aggcgaaagt gtctcttgtc ggagttcccg acaagcctgg tgttgccgca
      301 aagatttttg gagatattgc cgccaggaac atcaacgtag acatgatcat ccagaacgtc
      361 ggcagcgacg atcacaatga catctcgttc actgtcagcc gaagcgatct gaaggcagtc
      421 atagccattg ccaatgatct cgtgaaggag attcacgcac aaaaggttca atcggatgac
      481 aaagtcgtca aggtatccgc cgtcggagtc ggaatgcgtt ctcactcagg ggtggctgga
      541 aagatgttct cggcgctggc caacaaaggg atcaacattc agttgatcag cacctccgag
      601 ataaaaatat cctgcatcat tgctgaagag tacgcggaat tggcggtgcg ggcgctggcg
      661 gaagagtttg aattggagaa tgaatcaaag gagttggagc aatgacaaga cctagaaatg
      721 tacgtctgta tgatacgaca ttgagagacg gttgtcaggc cgagggtgtt tctttctcag
      781 ttcgtgagaa ggtcatgatc gcccacaaac tcgatgagtt tggcattcac tatatcgagg
      841 gcggatggcc gggatccaac cccaaggaca ttgagttctt tcaggaaatc tacaaggatc
      901 ctcccaagaa tgcgattatc acggcatttg gcagcacccg acgggcgaag gtcactgcag
      961 acaaagaccc caacctctgc gcgatcgttg agtccaaggt tcgtgccgcg gcgatctttg
     1021 gcaagtgctg ggtgcgtcac gtcc
//
LOCUS       DAOQUC010001643         1041 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_215032_length_1041_cov_3.185598, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001643 DAOQUC010000000
VERSION     DAOQUC010001643.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1041
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_215032_length_1041_cov_3.185598"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /locus_tag="PKH07_20565"
     CDS             <1..272
                     /locus_tag="PKH07_20565"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNT37399.1"
                     /translation="YLEKPAQVHWVSMGIYAYQPKALEYISQGEKLDFPDLVLRLLRA
                     GEKVHSYHDEKATWLDIGRPEDYEQAQNQAETLLESYLTNSKGGE"
     gene            265..519
                     /locus_tag="PKH07_20570"
     CDS             265..519
                     /locus_tag="PKH07_20570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008287891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HNT37400.1"
                     /translation="MSEGMGVQEQVIEIIVDKLKVDASEVSPEKHFTRDLGADSLDTV
                     ELVMEFEEKFGIDEIPEDEAEKIQTVGDAIAYIKEKTGQQ"
     gene            698..>1041
                     /locus_tag="PKH07_20575"
     CDS             698..>1041
                     /locus_tag="PKH07_20575"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HNT37401.1"
                     /translation="MYKRVVITGMGVITPVGSTLRDFWGALLAGKSGIGLLDRFDTTN
                     FSSKIAAQIKGFDPTQFIPEKEMTRIDPFVQYALGASQMALDDSGLDLSKVDPDNVGV
                     LVGSGIGGLKTI"
BASE COUNT          263 a          240 c          297 g          241 t
ORIGIN      
        1 attatctcga gaaacccgcc caggtgcatt gggtcagcat gggcatctat gcctatcaac
       61 ccaaggcctt ggagtatatc tctcaaggag agaagctgga ttttcctgac ctggttctgc
      121 ggttgctcag ggcaggtgaa aaggtgcatt cctatcacga cgaaaaagca acctggctgg
      181 acatcggacg acctgaagat tacgaacagg ctcagaacca ggcagaaaca ttgttggaaa
      241 gttatctgac caactccaaa ggaggtgagt gagggcatgg gcgttcaaga acaggtgatc
      301 gagatcatcg ttgacaagtt gaaggttgac gcgagtgagg tgtctcccga gaagcacttc
      361 acacgagacc tcggcgcgga ttccctggac accgtcgaac tggtgatgga gttcgaggag
      421 aagttcggta ttgatgagat tccggaagac gaagccgaga agattcagac cgttggcgat
      481 gccatcgctt atattaagga aaagacggga cagcagtgat cgttctctta acgggtttgg
      541 gtagtcagat tcgaatacat ttcgcatctg actacccgat cccaatggga gtgccatctc
      601 cggacagacc cggacgttcg gattgttcac actgggcatg tgaaggttcc agtgtcacat
      661 gtgcagttat tcagattcag gagatgcgag gaaccagatg tataaaagag tagtcattac
      721 cggtatgggc gtcatcacgc ccgttggctc taccctgcga gacttttggg gcgcgctttt
      781 ggcgggcaag tcaggaatcg ggcttcttga tcgcttcgat accaccaatt tctcctccaa
      841 gatcgcggca cagatcaaag ggtttgatcc cacccagttt attccggaaa aggaaatgac
      901 acgcattgac cccttcgtgc agtatgcttt aggggcaagc cagatggctc tggatgactc
      961 gggcttggat ctgtccaaag tggatcccga caatgtcggt gtcctggttg gctctggaat
     1021 cggtggactg aaaaccattg a
//
LOCUS       DAOQUC010001644         1039 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_215747_length_1039_cov_3.255081, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001644 DAOQUC010000000
VERSION     DAOQUC010001644.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1039
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_215747_length_1039_cov_3.255081"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1013)
                     /gene="hcp"
                     /locus_tag="PKH07_20580"
                     /gene_synonym="priS"
     CDS             complement(<1..1013)
                     /gene="hcp"
                     /locus_tag="PKH07_20580"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HNT37402.1"
                     /translation="MCGKRPDVAALQDLLIHAAEGISMYAHRAVQLGVRDREIDLFVI
                     EALFSTVTNVDFDPERLQTFLIKAGRLKNKAKALYEDACKKAGKPVESLEGPASWVPE
                     GDIPGLIQQGNRVSLADQNQRIGADRGGLYHVILFGIKGAAAYADHAQILGQEAEDLY
                     AGFHEVLNFLASEDATVDDLLKMALRVGAINLKAMELLDTANTGAYGHPVPTKVRIEP
                     VKGKAILISGHDLKDLEELLKQTDGKGINVYTHGEMLPAHGYPKLKKFKHLVGNYGGA
                     WQDQRDEFQKFPGAILMTTNCIQKPNDDYKGRIFTSGLVAWPGVVHISDGNFAPVIQA
                     ALEA"
BASE COUNT          225 a          315 c          258 g          241 t
ORIGIN      
        1 gcttcgagtg ccgcctggat gacaggagcg aaattcccgt ccgaaatatg aacgacacca
       61 ggccatgcta ccagtccgct cgtgaagatt cgtcccttgt agtcgtcgtt gggcttctgg
      121 atgcagttgg tggtcatgag gatcgcccct gggaacttct gaaactcgtc acgctgatcc
      181 tgccaggcac caccatagtt acccacaagg tgtttgaact ttttcagttt cggatagcca
      241 tgggcgggga gcatctcacc atgggtgtag acattgatcc ccttgccgtc cgtctgcttg
      301 agcagctctt caaggtcttt cagatcatgc ccggagatga gaattgcctt tcccttgacc
      361 ggctcgatgc gaaccttggt cggaacgggg tgtccatagg ctcctgtgtt ggctgtatcc
      421 agaagttcca tggctttgag attgatcgct cccacgcgca gcgccatctt caagaggtcg
      481 tccacagtgg catcctcact ggccaggaaa ttcaagactt catgaaagcc ggcgtacaga
      541 tcctctgcct cctgccccag aatctgggca tgatcggcat acgccgcggc ccccttgatc
      601 ccaaacagga tcacatgata gagcccacca cgatctgctc cgatgcgctg attctgatcg
      661 gccagagaga cgcggtttcc ctgctggatc aagccaggta tatcgccttc aggaacccag
      721 gaagcaggac cctcaagact ctcgacaggt ttccccgcct tcttacaggc atcttcatag
      781 agcgccttcg ccttgttctt cagacgtcct gctttgataa ggaaggtctg aagtctctca
      841 ggatcgaagt ctacgtttgt cactgtcgag aacaatgcct caatgacaaa aagatcaatc
      901 tcgcgatccc tgacacccag ttgaaccgcc ctgtgggcgt acatggaaat gccttccgcc
      961 gcatggatca acaaatcctg cagcgcagcc acatccggcc tcttgccaca cacgccactg
     1021 acagcgcatc ccttgccac
//
LOCUS       DAOQUC010001645         1038 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_216105_length_1038_cov_3.398779, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001645 DAOQUC010000000
VERSION     DAOQUC010001645.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1038
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_216105_length_1038_cov_3.398779"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..969)
                     /locus_tag="PKH07_20585"
     CDS             complement(<1..969)
                     /locus_tag="PKH07_20585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37403.1"
                     /translation="MTAKYMIRMCLCVVLFLSAFPILSMAKQPSLRAKAGAYYFEGWT
                     GKTFHLTERLKNEFFDREPVWGWNDDSRRIMEKQIKYAADNGLSFFAFDWYYPEEENK
                     ETPLNNGLEHYLRARSRKRMEFCLLVANHAGFRIGPKEWDIVSEKWIELFKQPTHLKV
                     NGKPLLIFFAPNELYKAFGGPEGVKQAFDSLRSRAVEAGLPGVMIAACTVPGPDDNRD
                     ILTGLEASGYDLLTGYNYHGHPARPGAPNLRPFAEMVEGHIGVYDRFAAKAKLPYIPV
                     ITVGWDKRPWEEYGLPEESQSVYYPDTTPHHRQLSHHPPGGPLDRPL"
BASE COUNT          229 a          288 c          259 g          262 t
ORIGIN      
        1 tagcggtcga tccaggggtc cacctggcgg gtggtgcgat aactgccgat ggtgaggcgt
       61 cgtgtcggga tagtagacag actggctctc ctctggaagt ccgtactctt cccagggtct
      121 cttgtcccaa ccgaccgtga tgacagggat gtaagggagt ttggcctttg ccgcaaagcg
      181 atcatagacc ccgatatgtc cctcgaccat ttcggcgaac ggtctcagat tgggagcgcc
      241 cggtcttgca ggatgtccat ggtaattgta tccggtcagg agatcgtaac ccgacgcctc
      301 aaggccagtc aggatatctc gattgtcatc aggaccaggt accgtgcagg ctgcaatcat
      361 gacaccagga agccccgctt caacggcgcg gctcctgagg ctgtcaaagg cctgtttgac
      421 cccctccggg cctccaaacg ctttgtaaag ttcattgggc gcaaaaaaga tcaacagcgg
      481 ttttccattg accttcaggt gcgttggctg cttgaacagt tcgatccact tctccgaaac
      541 gatatcccat tccttcggtc caatccgaaa tccagcatgg tttgccacca gaagacagaa
      601 ctccattctc tttcttgaac gcgcacgaag atagtgctcc agaccgttgt tgagaggcgt
      661 ttctttgttc tcttcctcag ggtagtacca atcgaacgca aaaaatgaca ggccgttgtc
      721 ggcggcatac ttgatctgct tctccataat cctgcgcgag tcgtcgttcc aaccccacac
      781 gggctcgcgg tcaaagaact cgttcttcag tctctcagtc agatgaaacg tctttcccgt
      841 ccatccttcg aagtagtagg cgcctgcttt ggcccttaga cttggttgtt tggccatcga
      901 aagtattggg aaagcactca ggaatagaac cacgcataga cacatccgaa tcatgtactt
      961 ggcagtcatt ggacagtctc cttctcaaga ctcttcttct tgatcgagtt tcttggctct
     1021 atcctaacac aaatgttg
//
LOCUS       DAOQUC010001646         1038 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_216107_length_1038_cov_3.357070, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001646 DAOQUC010000000
VERSION     DAOQUC010001646.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1038
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_216107_length_1038_cov_3.357070"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(46..600)
                     /locus_tag="PKH07_20590"
     CDS             complement(46..600)
                     /locus_tag="PKH07_20590"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HNT37404.1"
                     /translation="MGESILEAQGCHCSQMESGDLERICAIADRHVGSRGALIAMLES
                     IQQEQGYLPEGDLRLLSQVTGIALVDIYGVATFYRSFSLKPRGKHLICVCLGTACHVR
                     GGAAVVEELSRVLDIPVGETTLDGEFTLETANCLGACALGPIVVVDGQYFSKVKVTDA
                     KDIVAQFRDKADVIEPATEACTAA"
BASE COUNT          245 a          312 c          241 g          240 t
ORIGIN      
        1 aaatgctgaa atggatgtct gtcgttgcac gagtggttct tatccttatg ccgcagtgca
       61 ggcttcggtt gcaggctcaa ttacgtctgc cttgtcccgg aactgggcaa caatgtcttt
      121 ggcatcggtc acttttactt tggagaaata ctgcccatcc accacaacga tggggcctaa
      181 ggcacacgcg ccaagacagt tggcggtctc tagggtgaac tccccatcta atgtggtttc
      241 tcccacaggg atatccagga ctcgggacag ttcttccaca acggctgccc ctccccgcac
      301 gtgacaggct gttccaaggc aaacacaaat cagatgtttc cccctgggct tgagactgaa
      361 agagcgatag aaggtggcca ccccatagat gtcaacaagt gcaatacccg taacctgtga
      421 cagcagcctc aggtcgcctt ccggcagata gccttgttcc tgttggatgc tttccagcat
      481 ggcgatcaag gccccacggc tcccaacgtg ccgatcagcg atggcacaga tccgttccaa
      541 atcaccactt tccatttggc tgcaatgaca gccctgtgct tcaagtatac tttctcccat
      601 tgccccgact cctcaatgaa tggggatagc ccacgctcct gcgtgcacca cccgcctatc
      661 ggtactcctc acagtgccat actccgccat cactcttctg aaccagagag caaccagagc
      721 ggttttcgca atcaaaacac aacccgagat acctcttctc actaccacaa tcagtgtctg
      781 taagagcatg agaggtggcg gacgcgcgtc cctccctttg ggatgctcct cccgaagagg
      841 caggttcgac ctcaaatagt tcgcagaaca gcagaggcct catgctgcga ctctcaagaa
      901 gacactgcat ggaattccga caattgcagc aaagagtgaa gtcctttata ttcatcatgg
      961 tcttctgact ccttcccgtc tccacattgg cattgtttcg cagtaagtgt gccaggaagc
     1021 acttcggcag aagatcac
//
LOCUS       DAOQUC010001647         1038 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_216116_length_1038_cov_3.244151, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001647 DAOQUC010000000
VERSION     DAOQUC010001647.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1038
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_216116_length_1038_cov_3.244151"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..623)
                     /locus_tag="PKH07_20595"
     CDS             complement(<1..623)
                     /locus_tag="PKH07_20595"
                     /inference="COORDINATES: protein motif:HMM:NF012361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HNT37405.1"
                     /translation="MDYKDTLRLPQTDFPMKASLPQREPQIAETWTRLGLDSLPTEKD
                     KDKPRFYLHDGPPYANGHIHVGTALNKILKDIVLKGKALEGYSVPYIPGWDCHGQPVE
                     HAVVSKLGKEARSLSPSKIRSLCKDYALKFMDIQRQEFKRLGVLGDWDHPYLTLAPSY
                     EAQIYRLVARLVPYIYRSLKPVQWCRRCQTALAEAEVEYEDKSSPAI"
     gene            complement(658..>1038)
                     /locus_tag="PKH07_20600"
     CDS             complement(658..>1038)
                     /locus_tag="PKH07_20600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37406.1"
                     /translation="SMTYAGVKSYIYAGLTRDDPRVKAAYDWILTHYTVEENPGMGQA
                     GLFYYYHTFAKTLAVLGDLEITFPSGEKRVWGTDLSEKLLSLQKPEGYWINEQSSRWW
                     ENNKVLATCYAVLALVETSRAYGW"
BASE COUNT          220 a          285 c          274 g          259 t
ORIGIN      
        1 tggatcgccg gtgaagattt gtcctcgtat tcgacttccg cttctgcgag cgctgtctgg
       61 catcttcgac accactgaac cggtttgagg cttcggtaga tgtaaggaac cagccgtgcc
      121 acaagacgat agatctgcgc ttcataggaa ggcgcaagag tcaggtaagg atgatcccag
      181 tcgcccagta cgccaagcct cttgaactcc tggcgttgga tgtccataaa cttgagcgcg
      241 tagtccttgc acaggctgcg gattttggaa ggggacagag aacgggcttc cttgccaagt
      301 ttggagacga cagcgtgttc aacaggctga ccgtgacagt cccagccagg gatgtacggt
      361 acagagtatc cctcaagggc ttttcccttg agaacgatgt ccttgagtat cttgttcagc
      421 gctgtgccga cgtgaatgtg cccattggca tagggcggac catcgtgcag gtaaaacctt
      481 ggcttgtcct tgtccttctc tgtcggcaga gagtccagtc ccagtcttgt ccatgtctcc
      541 gcaatctgag gttcccgctg cggaagactt gccttcatgg ggaaatccgt ctggggtaac
      601 cgcaatgtgt ctttgtaatc caccgttcaa tcctcttctg tcaatagtac atcccgtcta
      661 ccaaccgtag gcgcgagacg tctccaccaa agccagcacc gcatagcagg tcgccagcac
      721 tttgttgttt tcccaccaac gactactctg ctcgttgatc caataccctt ccggtttctg
      781 aagtgacaac agtttctcgg aaagatctgt gccccatacg cgtttttcac cactgggaaa
      841 cgtgatttcc aaatcgccaa gcacggcaag agtcttggca aaagtgtgat agtagtagaa
      901 gagtcccgcc tgacccatgc cgggattttc ctctaccgta tagtgggtca gtatccagtc
      961 gtaggctgcc ttcactctcg ggtcgtcgcg tgtcagtccc gcgtagatgt aggacttcac
     1021 gccggcgtag gtcatgga
//
LOCUS       DAOQUC010001648         1036 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_216819_length_1036_cov_4.212029, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001648 DAOQUC010000000
VERSION     DAOQUC010001648.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1036
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_216819_length_1036_cov_4.212029"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(184..>1036)
                     /locus_tag="PKH07_20605"
     CDS             complement(184..>1036)
                     /locus_tag="PKH07_20605"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011305841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HNT37407.1"
                     /translation="QGIQNFTGEEADRMRGVDPDCATRDLHEAIARGEFPSWKLEVQI
                     MTPEQAKNYRFDPFDITKVWPHADFPPITVGRMVLNRNPENYFAEVEQAAFSPGNFVP
                     GIGPSPDKMLQGRLFSYHDTHRHRLGTNYHLLPINAPKACPMNSYQRDGAMRPDNNGG
                     DGPNYWPNTFGGAEPDPKLAPPPIDVSGMAARHAYQLGDVDFVQAGDLYRKVMSPTER
                     KHLVKNIVSHLKNAQKRLQFRQTAIFYKADSDYGQRVAEGLGIDLQQVKKLAAMSQED
                     RVKATLK"
BASE COUNT          219 a          273 c          278 g          266 t
ORIGIN      
        1 gttctcagtg ctcctgaacc gtggcgcact ttgggcggtt gatctgctgg aaccgaaatc
       61 ctgacggtgc actgcgtcaa gagttgaatt ctcgaccgac aaacttccat taaagcgaca
      121 acgggctgca cggaaatgca gcccgttgtt ctggagaacc aggagatttc actcgcctga
      181 gatctacttg agtgttgcct tcactctgtc ctcttgagac attgcagcaa gttttttgac
      241 ttgctgcaag tcaatgccaa gaccctctgc aacacgttgt ccatagtctg aatctgcctt
      301 gtagaatatt gccgtctgtc tgaattgaag acgtttctgt gcattcttga gatgggagac
      361 aatattcttc accaagtgtt ttcgttccgt gggtgacatg acttttcgat acaagtcgcc
      421 cgcctgtaca aagtccacat ctcccagttg gtaagcatgt cgagcggcca ttccggaaac
      481 gtcaatgggt ggcggcgcca gttttggatc cggctccgcc ccgccaaaag tgtttggcca
      541 gtagttcgga ccatccccac cgttgttgtc aggacgcatg gcaccgtccc gctgatagct
      601 attcatggga caggctttgg gcgcgttgat tgggagcaga tgatagttgg taccgagtct
      661 gtgccgatgt gtgtcgtggt aactgaacaa acgtccctga agcatcttgt cgggcgaagg
      721 cccgatgccc ggaacaaagt tccctggtga gaacgccgcc tgctccacct cggcgaaata
      781 gttctcagga ttgcggttca gaaccattct tccaacagtg atgggcggga agtctgcgtg
      841 tggccagacc ttcgtgatat caaaggggtc aaaacgatag ttcttggctt gttccggagt
      901 cataatctgc acttcgagtt tccaggacgg gaattcccca cgagcaatgg cctcgtgcag
      961 atcacgcgtg gcgcagtcag gatccacccc tcgcattcgg tcggcttcct caccggtgaa
     1021 attctggatt ccctgt
//
LOCUS       DAOQUC010001649         1036 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_216840_length_1036_cov_3.314985, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001649 DAOQUC010000000
VERSION     DAOQUC010001649.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1036
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_216840_length_1036_cov_3.314985"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1036
                     /locus_tag="PKH07_20610"
     CDS             <1..>1036
                     /locus_tag="PKH07_20610"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HNT37408.1"
                     /translation="KGPFEDASAPLIKDASSYIDAHVYFDETSGRHYFYVCRMGNGPG
                     AIWSARLSEDLLSLDTELTMVFSPDQPYDFGWVEAPFIVKHRDTYYMTYSRNGFSSSL
                     YGVCYATAPSPMGPWTKFDRNPILTRTHEVSGPGHNCLVNSPDGRELFIAYHTHLSFA
                     GGGHRQLAIDRLEFVPNGDQPDRLQLANGIPSLNPQPLPSGSVPMRVGRSDDFESSDL
                     DWTRWSIYFHEPGKWRLNDGNLIIETVEGDTHRERSDAANIFLQYAPHKDFRIRTKVN
                     FTPLENHEQAFLMIWQDCNNYIRFSSLYANGQNLEIGCELNGVYDAFLIPNELLPEVF
                     LQIEKKGTRYL"
BASE COUNT          243 a          290 c          261 g          242 t
ORIGIN      
        1 aaaggtccct ttgaggacgc atccgcaccc ctgatcaaag acgccagcag ttacattgat
       61 gctcatgtgt atttcgatga gacgagcggg cgacactact tctacgtgtg tcgtatggga
      121 aacggtcctg gcgccatctg gtctgcacgg ctcagcgaag accttctctc tctggatacg
      181 gaactcacga tggtcttcag tccggatcag ccttacgact ttggatgggt ggaagcgccc
      241 ttcattgtca agcatcgaga cacctattac atgacctatt cgcgaaatgg ctttagctcg
      301 tctctttacg gggtctgcta cgccacagca ccttctccca tgggaccgtg gacgaagttt
      361 gatcgaaatc ccattctgac tcgaacgcat gaggtctccg gacccggaca taactgtctg
      421 gtcaactctc cggatggcag ggaactcttc atcgcctatc acacgcattt gtcatttgcc
      481 ggtggcgggc atcgtcaact tgccatagac cgtctggagt tcgttcccaa cggagaccaa
      541 ccggatcggc tgcaactggc aaatgggatt ccttcgctga atccacagcc gcttccctct
      601 ggaagcgtgc cgatgcgagt cggacgctcg gatgatttcg agtcttcaga cttggattgg
      661 acacgctggt caatctactt ccacgagcct ggcaaatggc ggctgaatga cgggaatctc
      721 atcatcgaga cggtggaagg agacacccat cgggaacgaa gtgacgccgc gaatatcttt
      781 cttcagtacg ccccacataa ggatttccga atccgaacca aggtcaactt cacacctttg
      841 gaaaaccatg agcaggcgtt tctgatgatc tggcaggact gcaacaacta cattcgattc
      901 tcatccctct acgccaacgg tcagaacctg gagatcggct gcgaactcaa cggcgtctat
      961 gatgcctttc tcattccgaa cgaactgctg ccagaagtct ttctgcagat cgagaagaag
     1021 ggaacaagat acctgt
//
LOCUS       DAOQUC010001650         1036 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_216849_length_1036_cov_3.214067, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001650 DAOQUC010000000
VERSION     DAOQUC010001650.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1036
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_216849_length_1036_cov_3.214067"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..473
                     /locus_tag="PKH07_20615"
     CDS             <1..473
                     /locus_tag="PKH07_20615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004338265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37409.1"
                     /translation="RALSTSHLYSSAFYVEKELGANGLPSKFRLHGAGWGHGVGLCQI
                     GAAVMAHRGYDFREILAHYFAGTTLVGLYGTASQDAPDRDLLIAPHAGLKDLLCWEVL
                     NCYDAKECSVALQGLGARCWEQPGTRCQAEARSKGLEPLSVCSQCCFYRMKHEN"
     gene            526..>1036
                     /locus_tag="PKH07_20620"
     CDS             526..>1036
                     /locus_tag="PKH07_20620"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HNT37410.1"
                     /translation="MNSITVVALLCRENQDLLEGLKTVLAQDVAELDVLVMDLCDSAA
                     SKALCAQAQQIDPRVRVIAGKRNNLAQACNAAREASKADYLVNLPANTRFYPGMLKKY
                     VDILAGNTNLAFAYSNFESQKGEEKQLKKLLEDIGDNTEMADFGHVKMFRTACLKEVG
                     GYREDFNHAE"
BASE COUNT          251 a          250 c          305 g          230 t
ORIGIN      
        1 gacgagcact ttcgacatcg catctttaca gttcggcatt ctatgtggag aaggaattgg
       61 gtgccaacgg cctgccgtca aagttccgtc ttcacggtgc cggatgggga cacggagtgg
      121 gactgtgtca gatcggggcg gcagtgatgg cacatcgcgg atacgatttc cgtgagattc
      181 tggctcatta ctttgccgga acaacgcttg tcggtctgta cggcacggcc agtcaggatg
      241 ctcccgatag agaccttctc attgcccctc atgccggtct gaaggatttg ctatgctggg
      301 aagtgctaaa ctgctatgat gcgaaagaat gttctgtcgc gttacagggc ttgggtgccc
      361 gatgctggga gcaaccggga acccggtgtc aagcggaagc acgctccaaa ggactggagc
      421 ctttgagtgt gtgcagtcaa tgttgttttt atagaatgaa gcacgaaaac tgaactcccg
      481 aacccagttt gggagacacc aaaagcagga aggataggaa agaacatgaa ctcgattact
      541 gtggttgcgt tgctatgtcg agagaaccag gatctgctgg aaggtctcaa gactgttctg
      601 gcacaggatg ttgcggagtt ggacgtgctc gtgatggatc tgtgtgactc cgcagccagc
      661 aaagcgcttt gcgcacaagc ccaacagata gatccgcgag ttcgggtcat tgccggaaaa
      721 cgcaacaatc ttgcccaggc ttgcaacgcc gctcgggaag cctccaaggc agactatctg
      781 gtcaacctgc ctgccaacac ccgcttctat ccgggaatgt tgaagaagta cgtggacatt
      841 ctggctggca atacgaattt ggctttcgcc tactcgaatt tcgaatcgca gaagggcgag
      901 gaaaagcaac tcaagaagct ccttgaggac atcggtgaca acacggaaat ggctgatttc
      961 ggtcatgtca agatgttccg cacggcgtgt ttgaaagaag tcggcggcta ccgcgaggac
     1021 ttcaaccatg ccgagg
//
LOCUS       DAOQUC010001651         1036 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_216886_length_1036_cov_2.727829, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001651 DAOQUC010000000
VERSION     DAOQUC010001651.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1036
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_216886_length_1036_cov_2.727829"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..908)
                     /locus_tag="PKH07_20625"
     CDS             complement(<1..908)
                     /locus_tag="PKH07_20625"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNT37411.1"
                     /translation="MLHKEDPSEKKINNYLEKAVAAIKSGEFDFARQYVQHALKLQPD
                     HPEARTLLERIPIERARRWNAVSRLIQGATVLLKVVFRSYESSVGVAEALAKSRPGHV
                     FWFRIFGYCCIKLARWTDAIEAYETVVRHKSTDIRALVQLGEMYVMTGRYAEAESTYA
                     SAVRLRPQDQNLIRAHKNATAQLYSKTGVPERLTEKRALEEKGKIEEQKEREAKHQET
                     LLKLRESCTNDPKDFRSRARLGRMLAEEEQFAEAAKFLAEASLLSPKDIKILRQLADC
                     QERSHQYKAALDTWQLLLREDPEDSQA"
BASE COUNT          206 a          273 c          274 g          283 t
ORIGIN      
        1 gcctgagagt cttcgggatc ctctctcagt aagagttgcc atgtatccaa ggcggctttg
       61 tactgatgcg agcgttcctg acaatcggca agttgtctca atatcttgat gtcctttgga
      121 cttaagaggg atgcttcagc aaggaacttc gccgcttcgg caaactgctc ttcctcagca
      181 agcattctgc ccaagcgcgc gcgactgcgg aaatccttcg ggtcattggt gcaggattcg
      241 cgcagtttca gaagggtctc ctgatgcttg gcttcccgtt ccttttgttc ctctatcttg
      301 cccttttctt cgagtgctct cttctcagtc agcctttccg gtacaccggt cttcgaataa
      361 agctgtgccg tggcattctt gtgggcgcgt atgagattct gatcttgtgg acgaaggcga
      421 accgcggaag cataggtgct ctccgcttcg gcatatcggc ctgtcatgac gtacatttca
      481 cccaactgta ctaaggcgcg gatgtccgtt gacttatgtc tgacaactgt ttcgtaggct
      541 tcgatggcat ctgtccagcg ggctagcttg atacagcagt agccaaaaat ccgaaaccag
      601 aagacatgtc cagggcgact ttttgccagc gcttccgcga caccaactga ggactcatag
      661 gagcgaaaga cgaccttgag aagaacggtc gcgccctgta tgaggcgact cacggcgttc
      721 caacgtcgag cacgctcgat ggggatgcgc tccaggagtg tccgcgcttc agggtgatca
      781 ggttgaagtt tgagagcgtg ctgaacatat tgacgagcga aatcgaactc ccccgacttg
      841 atcgccgcta cggccttttc cagatagttg ttgattttct tctcagaagg atcttctttg
      901 tgaagcacgg tttgtactcc cttgctcagc gatttgcagc ctgcctgaca taggctgtga
      961 tcctttccat cgtgttctgc atgtctgagt tcttgccgag gctgctctca atctttcggc
     1021 aggagtatag gacagt
//
LOCUS       DAOQUC010001652         1035 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_217367_length_1035_cov_1.933673, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001652 DAOQUC010000000
VERSION     DAOQUC010001652.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_217367_length_1035_cov_1.933673"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..489)
                     /locus_tag="PKH07_20630"
     CDS             complement(<1..489)
                     /locus_tag="PKH07_20630"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNT37412.1"
                     /translation="MNALIKHCLVRAMAVLSDAFPKSTVSLYRLLWREKPGRFHLIHW
                     AAFTDRLQMVDALVQRDCDINAVDFRGMTPLCYAADNDSLATMAYLIQHGAELDKTDM
                     CGNTPLHHAALRGRSAAAKMLIERGADVNALHEDHWTPLHFAAEAKHTLLVRLLVAAG
                     ADI"
     gene            complement(509..>1035)
                     /locus_tag="PKH07_20635"
     CDS             complement(509..>1035)
                     /locus_tag="PKH07_20635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37413.1"
                     /translation="TERDREYGPGDVIERVKDLPPEIEDRQEFDQDLLDAGVSIEPGE
                     KVLGYSEGSRRPIHVTTDHLEIPKTLIHERTHQLSHPAARERLGAPLHEGITEDIAIL
                     NLGSEPIEGIPQCYPEERAIARQLRLLCGEEAINEAYFRGDLQRLRERLESHSGKGAL
                     DKLRRRMEKLDNIE"
BASE COUNT          229 a          292 c          254 g          260 t
ORIGIN      
        1 gatatccgct ccggcagcga ccaggagtct cacgagcaga gtatgttttg cttcagcggc
       61 aaagtgcaga ggcgtccagt gatcctcatg aagggcattg acgtctgctc ccctttcgat
      121 gagcatcttc gccgcggcgc tgcgtcctct tagagcagca tggtgcaaag gggtattccc
      181 gcacatatcc gtcttgtcca actcagcccc atgctgaatc aggtaagcca tcgttgcgag
      241 agaatcgttg tcggcagcat agcagagtgg agtcattccc cgaaagtcca ctgcattgat
      301 gtcacagtct ctttggacaa gcgcatcaac catttgaagg cggtctgtga atgcagccca
      361 atggatcaga tgaaagcgtc ctggcttctc acgccatagc agacgataca aagacacagt
      421 agatttagga aatgcgtcgg atagaacagc catggctctc acaagacagt gtttaatcag
      481 cgcgttcatt actgcaacct ccctgaattc attcgatgtt gtctaacttc tccatccttc
      541 tgcgaagttt gtcaagagcc cccttacctg aatggctctc aagcctctct ctcagtctct
      601 gcagatcccc cctgaagtaa gcctcattga tggcttcctc accgcacagg aggcggagct
      661 ggcgcgcaat ggctcgttct tcaggatagc actgtggtat tccctcaatc ggctcagagc
      721 ccaggttcag gatcgcgatg tcctcggtga ttccctcgtg caaaggagcg ccaagacgct
      781 cacgtgccgc tggatgcgag agttgatggg tcctttcgtg aatgagtgtc tttggaatct
      841 ccaaatgatc tgtagttaca tggataggtc ttcggcttcc ctcggagtaa ccaagcactt
      901 tctcccctgg ttcaattgag acacccgcat ccaacaggtc ttgatcaaat tcctgacggt
      961 cttcgatttc cggaggcagg tctttcactc gttcaatgac atcccctgga ccgtactccc
     1021 tgtcacgctc tgtca
//
LOCUS       DAOQUC010001653         1034 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_217586_length_1034_cov_2.637385, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001653 DAOQUC010000000
VERSION     DAOQUC010001653.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1034
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_217586_length_1034_cov_2.637385"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..777)
                     /locus_tag="PKH07_20640"
     CDS             complement(<1..777)
                     /locus_tag="PKH07_20640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37414.1"
                     /translation="MSLSRKNHCVHTTFLALLIALPYIPALLGKARLVGGDTVNQFIP
                     WIQFFAHEVRDGRFPRWDPHSFCGTPFFANAQVAFFYPFTWLAFLVSVETFLPLYYLA
                     HGIGAGVAMYCWLHRRLRLPQAAFVGSLMWAWCGIAAGYRFAGFMAWAGATVYIPLAF
                     LLFDLWNERHQIVHGLLLTAVLALQLHAGHPQPCLFTFYFLLAYACWQALSSRSQTLK
                     ARQRLTMISIVPLCFAGALLLAAVILLPLVEVSLFTAARQG"
BASE COUNT          299 a          259 c          285 g          191 t
ORIGIN      
        1 accttgtcgt gccgcggtaa acagagagac ttccaccaag ggaagcagaa tcactgccgc
       61 aagcagcaaa gctcccgcaa agcagagagg aacgattgag atcatggtca aacgctgtcg
      121 ggctttcaag gtttgacttc tcgaagagag agcctgccag caggcgtacg cgagcagaaa
      181 gtagaaagtg aacagacagg gttgtggatg accggcatga agttgaagag caagcactgc
      241 agtcaacaac agaccgtgga caatctgatg gcgttcgttc cacagatcaa agagcaggaa
      301 agccagtgga atgtagaccg ttgcgccagc ccacgccatg aagcccgcga atcggtagcc
      361 cgcggcgatg ccacaccacg cccacataag cgaaccgaca aaggcggctt gcggaagccg
      421 gagtcttctg tgcagccagc agtacatcgc gacacccgct ccaatcccat gcgccagata
      481 gtaaagaggc aggaacgttt ctaccgagac cagaaaagcg agccaggtaa atgggtagaa
      541 gaacgcgacc tgcgcattcg cgaaaaaagg cgttccgcag aaggaatgtg gatcccaccg
      601 cggaaaccgc ccatcgcgca cttcgtgggc gaagaattgg atccagggga tgaactgatt
      661 taccgtatcg ccgccgacga gtcgcgcttt ccccaagagc gctggaatgt aaggcagtgc
      721 aatgagcaat gcaagaaagg ttgtatgaac acaatgattc ttcctgctca gactcacaga
      781 cggcaactcc tgaatctgcg tccaatcaaa gcgattctgc gcctgtcttc tcctcggaca
      841 tcgttatcct ataggttcgt gtatgtggaa cgcaagcgaa tcataagaaa agtattgaca
      901 gcatgtttta gataccctat attacctata ggaaataagg agtatgaaga gaagtgctca
      961 gtttatcctg gcattgagcc tgcaggggct gtagtatctg ccaagatggg cgctgaatcc
     1021 aaagggacag attt
//
LOCUS       DAOQUC010001654         1034 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_217730_length_1034_cov_1.854954, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001654 DAOQUC010000000
VERSION     DAOQUC010001654.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1034
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_217730_length_1034_cov_1.854954"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1034
                     /locus_tag="PKH07_20645"
     CDS             <1..>1034
                     /locus_tag="PKH07_20645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37415.1"
                     /translation="PSNILDFSPKPVSHPLPLESDPAITRQALKVIDCVLDAPEQQDS
                     PFVNAFGDISDDLLLEIIQMVLFAEPSPAMEASACLSLLPTRKVDIMLDTLLRSPNID
                     PVRRCILETIVKERNEAYKLLGQEGFALNLPENPEVALRRLIERTEEIDLPIEEAWAL
                     LSEEAMVSCIDLGYQATIPAFGRLLAHVADLVPTSVALKILQKMTQVSQKHLSLLVRA
                     LAMHPDERVRNALRAQEKWLTKHGISLNEILFCESKQPEVQVYLCSTEASGQYTMLVN
                     QRSSLQRLRSVFFHIDTWLQGLCLTQGTSCAMVSDDVAKMRVAEFLEDQGVHAVEQTD
                     ISLARARRII"
BASE COUNT          254 a          273 c          265 g          242 t
ORIGIN      
        1 cgccttcaaa catacttgat ttcagtccca aaccagtttc acatcctctc cctctggaat
       61 ccgatcctgc aatcacgcga caggcgttga aggttattga ctgcgttttg gatgcgcccg
      121 agcaacagga ttcccctttt gtgaacgcct ttggggatat ctcagatgac ttgttgctcg
      181 agatcatcca gatggtgctt tttgccgaac cgagtcctgc gatggaggcc tcagcgtgcc
      241 tgagtcttct tccgacgagg aaagtggata tcatgctgga cacgctcctg cgttcaccga
      301 atatagatcc ggtcaggcgg tgcatcctgg agacaattgt caaggagcgg aatgaggcct
      361 acaaactact gggacaagaa ggttttgccc tgaatctgcc cgaaaatcca gaagtagcgt
      421 tacgacggct gatcgagagg accgaagaga tcgatctgcc catcgaagag gcatgggcac
      481 tgctctccga ggaagccatg gtctcctgca ttgacttggg gtaccaagcg acaataccag
      541 ccttcggaag actcttggcg catgtggcag atcttgtacc cacatccgtc gcgctgaaga
      601 tactgcagaa gatgacacaa gtctctcaga agcacctcag cctgttggtt cgtgctctgg
      661 ccatgcatcc agacgaaaga gtccgaaacg ctctccgagc ccaggagaaa tggttgacga
      721 agcacggcat ctctctgaat gaaatcttgt tttgtgaatc caaacaacca gaagttcagg
      781 tctatctctg cagcacagag gcatcgggac agtacaccat gttggtcaac cagagatcat
      841 ctctgcaaag gctccgaagc gttttcttcc atattgatac ctggcttcag ggtctttgcc
      901 tcacccaagg gacttcatgt gcgatggttt ccgacgacgt tgccaagatg agagttgctg
      961 aattcctgga agatcaggga gtacacgctg tcgagcaaac cgacatctca cttgccagag
     1021 cgcgtcgaat catt
//
LOCUS       DAOQUC010001655         1031 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_218559_length_1031_cov_4.247951, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001655 DAOQUC010000000
VERSION     DAOQUC010001655.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1031
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_218559_length_1031_cov_4.247951"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..467)
                     /locus_tag="PKH07_20650"
     CDS             complement(<1..467)
                     /locus_tag="PKH07_20650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37416.1"
                     /translation="MEELAQIVVFLIAGAVYLFAQIEKQKKAKQRQDEARKPKQVPRV
                     PKQSGSGGRRPTQSREQLSPIEVVKRHVAQGQIPVRVTNVHGPKRTEDPAEWREPVKP
                     KPHPVQRRDKRAAAPSKQKGDFLVFNDNALMKAIVLSEILQPPLAKRVPRQAG"
     gene            complement(494..>1031)
                     /locus_tag="PKH07_20655"
     CDS             complement(494..>1031)
                     /locus_tag="PKH07_20655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flotillin-like FloA family protein"
                     /protein_id="HNT37417.1"
                     /translation="LDRLVGGATEETIIARVGEGIVTTVGSAQSHKQVLENPDLISRS
                     VLQKGLDAGTGFEILSIDIADVDVGANIGAALQIEQANADKEIARAKAEERRAMAVAE
                     EQEMKARTQEMRAKVVEAEAEIPQAIAEAFRSGHLGIMDYYNMKNVISDTQMRQSIAD
                     SQTSHKSENSGGSADSHV"
BASE COUNT          213 a          298 c          248 g          272 t
ORIGIN      
        1 aatccagcct gtcttggaac acgctttgca agtggtggct gaaggatttc actcagcaca
       61 atcgccttca tcagggcatt gtcgttaaag acaagaaaat cccccttctg ttttgatgga
      121 gccgctgctc gtttgtcacg cctctgaaca ggatgtggtt tgggtttgac aggctcacgc
      181 cattctgcgg gatcctctgt ccttttgggc ccgtgaacat tagttacgcg taccggaatc
      241 tgaccctgag ccacgtgccg cttcacgact tcgatcggac tcagttgttc cctcgactga
      301 gtagggcgtc tacccccgga accagattgc ttgggaactc ggggaacctg cttcggtttt
      361 cgagcctcat cttgacgctg tttcgccttc ttctgcttct cgatctgagc aaaaagataa
      421 accgccccag cgatgagaaa tacgactatt tgagccagtt cttccataga gttctttgtt
      481 ctcgaccttc ggcctagacg tgagaatccg cactgcctcc agaattttcg ctcttgtgag
      541 atgtctggct gtcggcaatg gactggcgca tctgtgtgtc agagataacg ttcttcatgt
      601 tgtagtagtc cataatccca aggtggccac ttctgaacgc ctcagcgatg gcttggggga
      661 tctccgcctc tgcctcgaca actttggcac gcatctcctg agttctggcc ttcatctcct
      721 gctcctcggc gacggccata gcacggcgct cttctgcctt cgcgcgagca atctccttat
      781 ccgcattcgc ctgctcaatt tgaagtgccg ctccaatgtt ggcacccacg tctacatcgg
      841 caatgtcaat ggacaaaatc tcaaaaccgg ttccggcgtc caaacccttc tgaagaacag
      901 aacgcgatat cagatcggga ttctcaagaa cctgcttgtg agactgagcg gaaccaacgg
      961 ttgtcacgat tccttcaccg acgcgggcaa tgattgtctc ttcggtggca ccaccgacaa
     1021 gtctatccaa a
//
LOCUS       DAOQUC010001656         1029 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_219439_length_1029_cov_2.456879, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001656 DAOQUC010000000
VERSION     DAOQUC010001656.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1029
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_219439_length_1029_cov_2.456879"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..717
                     /locus_tag="PKH07_20660"
     CDS             <1..717
                     /locus_tag="PKH07_20660"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNT37418.1"
                     /translation="IGRLAAGIAHEVNNPLTAVLTFAHLLRESPKADDDQKQDLDVIV
                     RETTRVREIVRGLLDFARESPSRRQILDINEILQRTLSLLKSQKDFGKIKIDLCLQDS
                     LPPVMGDRNQMQQVFLNLSLNACEAMPEGGVLTVTTRQEESTVRVYFADTGCGISQEF
                     LTQIFDPFFTTKPVGKGTGLGLSVSYGIIQQHGGQLLVNSTPNEGSTFTVVLQVCTDP
                     VYLIEDEPEADRGAATPQQG"
     gene            725..>1029
                     /locus_tag="PKH07_20665"
     CDS             725..>1029
                     /locus_tag="PKH07_20665"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNT37419.1"
                     /translation="MNDPAQKSSDSSQRILVIDDEAIICLSCERILASDGHKVDFRND
                     AREGLETAIKGNYDIILLDLMMPDLGGLELLDLLRQAGVTSEVIIITGHAAVQTAVEA
                     "
BASE COUNT          249 a          245 c          287 g          248 t
ORIGIN      
        1 atcggacgac tggccgcggg tattgcccat gaggtcaaca atccattgac ggcagtcctg
       61 actttcgccc atctgcttcg ggaatcgccc aaagccgatg acgatcagaa acaggatttg
      121 gatgtcatcg tcagagagac cacgcgcgtt cgggagattg ttcgtggcct tcttgatttt
      181 gcaagggagt cgccttcgcg aagacagatt cttgacatca atgaaatcct tcaaagaacg
      241 ctgagtctgc tcaagagtca gaaggatttt ggaaagataa agatcgatct ttgcctgcag
      301 gacagtcttc cacctgtgat gggtgaccgc aatcagatgc agcaagtttt tctgaatctg
      361 tctctgaatg cctgtgaagc gatgcccgaa ggaggcgtcc ttaccgtgac gacgagacag
      421 gaggagtcta ctgttcgagt gtattttgca gatacgggct gtggcatttc ccaagagttc
      481 ttgacccaga tcttcgatcc attcttcacg acaaaaccgg tggggaaggg aacgggcttg
      541 gggctgagcg tcagttatgg cattatccag cagcatggag gacagctatt ggtgaacagc
      601 actccaaacg aaggttcgac attcacggtt gtgctccagg tttgcaccga tccggtgtat
      661 cttatcgaag atgagccgga agccgatcgg ggtgctgcga ctcctcaaca gggctgagtt
      721 tctcatgaat gatcctgcac aaaaaagttc tgatagcagt caacgaattc tcgtcattga
      781 cgatgaggcc attatctgtc tcagctgtga acgcattctc gcgagcgacg gacacaaagt
      841 ggatttccgt aacgatgccc gtgaagggct tgagactgcc atcaagggca actatgacat
      901 cattctgctt gatctgatga tgccggatct gggagggttg gaactgctcg acctgctccg
      961 tcaggctggg gtcacctcgg aagtgataat catcacgggt catgctgctg tacagaccgc
     1021 agttgaagc
//
LOCUS       DAOQUC010001657         1028 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_219730_length_1028_cov_3.084275, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001657 DAOQUC010000000
VERSION     DAOQUC010001657.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1028
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_219730_length_1028_cov_3.084275"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..441)
                     /locus_tag="PKH07_20670"
     CDS             complement(<1..441)
                     /locus_tag="PKH07_20670"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HNT37420.1"
                     /translation="MECACAHILVCTGSGCIASGALEVKAALERAIEEHGLGSECKVI
                     ETGCLGPCAVGPVMVTHPDGVFYQEVKAEDAEEIVKEHLLKGRIVDRLVHKEPARGES
                     VPAIQEIGFFKRQVKIVLRNCGVIDPTSIEEYIARDGYQALSKAL"
     gene            complement(468..839)
                     /locus_tag="PKH07_20675"
     CDS             complement(468..839)
                     /locus_tag="PKH07_20675"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HNT37421.1"
                     /translation="MVQKITNPADLLALQKKAQADIELRSGQKDMRITVHMGTCGIAA
                     GARDVLAYFVTELSRAQVQSVSLQRSGCAGLCDQEPMITLADKAGNEFRYGKMDKDKV
                     REIVDAHVVAGSPVTKYLIQT"
     gene            complement(842..>1028)
                     /locus_tag="PKH07_20680"
     CDS             complement(842..>1028)
                     /locus_tag="PKH07_20680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002568593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HNT37422.1"
                     /translation="TVGETTEDRVFTLEVARCFGSCGLAPAIMIDEDVHQRVKPARIE
                     AILEPYREKEVQSKERQ"
BASE COUNT          217 a          319 c          242 g          250 t
ORIGIN      
        1 cagtgctttg gaaagtgcct ggtatccgtc ccgagcgatg tattcctcaa tggatgtggg
       61 atcaatcaca ccgcaattgc gcaaaacgat cttgacctgt cttttgaaga atccgatctc
      121 ctggatggcc ggaacactct caccacgggc aggctctttg tgaaccagac gatccacaat
      181 tcgacccttg agcagatgtt ccttgacgat ctcctcggcg tcttcggctt tgacttcctg
      241 ataaaagacg ccgtcgggat gtgtcaccat cacggggcca accgcacaag gccccagaca
      301 accggtctcg atgactttgc attcgctgcc aaggccgtgt tcttcaatgg cacgctccag
      361 cgccgccttg acttccaagg ctcctgaagc gatgcagcct gagcctgtgc agacgagaat
      421 atgagcacat gcgcattcca tcgtttgctt ttccttcctg tatcgctcta agtttgaatc
      481 agatactttg tcaccggaga accggcaacc acatgcgcgt ccactatctc gcgcaccttg
      541 tccttgtcca tcttcccata gcgaaactca ttgcctgcct tgtcggccaa ggtgatcatc
      601 ggttcctgat cacacagtcc ggcgcaaccc gaacgctgca aggagacact ctgaacctgc
      661 gctcggctga gttcggtcac aaaataggcc agcacatctc tcgctcccgc agcaatgcca
      721 caggttccca tgtgaaccgt gatccgcatg tctttctgac cggatcgcag ctcaatgtcc
      781 gcctgagctt tcttctgcag tgccaggaga tctgccgggt tggtaatctt ctgaaccatg
      841 gtcattgcct ctctttcgac tgcacttctt tctcccgata gggctcaagg atggcttcaa
      901 ttcgcgcagg tttgaccctc tggtggacgt cttcatcaat catgatcgcc ggtgccagac
      961 cacaggagcc aaaacatcga gccacctcaa gtgtgaagac ccgatcctcc gtggtctcgc
     1021 caacagtc
//
LOCUS       DAOQUC010001658         1027 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_220067_length_1027_cov_3.238683, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001658 DAOQUC010000000
VERSION     DAOQUC010001658.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1027
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_220067_length_1027_cov_3.238683"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(221..523)
                     /locus_tag="PKH07_20685"
     CDS             complement(221..523)
                     /locus_tag="PKH07_20685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006933140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase N-terminal
                     domain-containing protein"
                     /protein_id="HNT37423.1"
                     /translation="MLNEFVAKMDWWALFGLFAQAAFTARFLIQWIASERAQKSIIPI
                     SFWYLSIAGSTGLLIYAIARTDPVFALGYLFNWIIYLRNLILIRRERSTTPSLSRS"
     gene            complement(513..>1027)
                     /locus_tag="PKH07_20690"
     CDS             complement(513..>1027)
                     /locus_tag="PKH07_20690"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNT37424.1"
                     /translation="TSALDCALRRARGKYIASLDADLQNDPKDIPTMLVPIVSGKCDM
                     VNGWRKDRRDPWIRLVSTKIANGVRNWLTHETIRDSACGLKVFRRECIERVKLYKGMH
                     RFLPTLVKMEGFRVIEVPVNHRPRTAGKTKYSIGNRLFRALRDAFAVRWMQSRTVLYQ
                     YEEWEREDAE"
BASE COUNT          253 a          288 c          254 g          232 t
ORIGIN      
        1 cccaaggacg ccttccttct gtgcgccatc gggagaggtt gcaccggctt tttcgctgag
       61 attctccacc tctgtgtgat accagagcac tccaagcact ccgaggatca ggagaagtaa
      121 taccaccatg attttgccca tagcgaacac ctttatgtgg cagcagccgt gaacgtcaac
      181 cgctcaaaca acttgggtct atcacggcaa ccaatggatg tcaagaacgc gaaaggctgg
      241 gggtggtact gcgttctcga cggatgagga tgagattcct caggtagata atccagttga
      301 acagatagcc caaggcaaag accggatccg ttcgcgcgat ggcgtagatg agaagtccag
      361 tacttcccgc aatgctcagg taccagaagg agatcgggat gatgctcttt tgggcgcgct
      421 ccgaggcgat ccattgtatg agaaatcgtg cagtgaaagc cgcctgcgca aacagaccaa
      481 acaatgccca ccagtccatt ttggcgacaa actcattcag catcttcccg ctcccattct
      541 tcgtattggt agaggacagt ccgactttgc atccatcgaa cagcgaaggc atcacgcaag
      601 gctcgaaaca gtcggttgcc aatactgtat ttcgtcttgc ctgcagttct tggacgatga
      661 ttcacaggca cttcgataac ccgaaagccc tccatcttga ccagtgtcgg cagaaaccga
      721 tgcatgccct tgtacagttt cacccgttca atgcactcgc gacggaagac cttcagtccg
      781 cacgcggaat cacgaattgt ctcatgtgtc agccaattgc gcacaccgtt ggcaatcttt
      841 gtggaaacca atcgaatcca tggatcgcgt cggtcttttc gccaaccatt taccatgtcg
      901 cattttccag acacgatcgg gacaagcatg gtcgggatat ccttggggtc gttctgcaaa
      961 tccgcatcca aactcgcgat atactttcca cgtgcccgac gcagggcaca gtccagtgcg
     1021 gaagtct
//
LOCUS       DAOQUC010001659         1027 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_220203_length_1027_cov_2.110082, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001659 DAOQUC010000000
VERSION     DAOQUC010001659.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1027
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_220203_length_1027_cov_2.110082"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1027)
                     /gene="gcvPB"
                     /locus_tag="PKH07_20695"
     CDS             complement(<1..>1027)
                     /gene="gcvPB"
                     /locus_tag="PKH07_20695"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018125193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HNT37425.1"
                     /translation="DVGEKANIRESLLREKPPALPELSELDVVRHFTELSRRNFGVDN
                     GFYPLGSCTMKYNPKVTEKIAQYPGFAYLHPLMPQLPGGDMMVQGALEVLHEMDRLLR
                     EITGMKAFTMQPLAGAHGELTGVMLMAAYHKDKGNKKTTVLIPDSAHGTNPASAAIAG
                     YSVQTVPSDSSGVMSVEALRQALGTHVAGLMLTCPNTLGLFNPRVREMCDLVHGVDGV
                     IYCDGANLNALLGNCRPGDIGFDIVHVNLHKTFATPHGGGGPGAGPVGVSERLVDFLP
                     VSVIYEKADGSYGLDYDRPKSIGYIAPFYGNFSIILRAYAYILMLGKEGLKRVSGNAV
                     LNANYVK"
BASE COUNT          257 a          292 c          258 g          220 t
ORIGIN      
        1 tccttcacgt agttggcgtt cagtacagca ttccccgaaa cacgcttcag accctccttg
       61 ccgagcatga gaatataggc ataggcgcga aggatgattg agaaattacc atagaatgga
      121 gcgatgtacc cgatagactt ggggcgatca tagtccaaac cgtaggaacc atcggctttt
      181 tcgtagatta ccgagacagg aagaaagtcc acaagccgtt cggaaacacc gaccggtccg
      241 gcaccaggtc caccgccacc atgaggcgta gcgaaagtct tatgcagatt cacatgaaca
      301 atgtcgaaac cgatgtctcc cggacgacaa ttgcccaaca gggcattcaa gttggcacca
      361 tcgcagtaga tgacgccgtc cactccatga accagatcgc acatctcccg aacgcgtgga
      421 ttgaacaagc cgagcgtatt gggacaggtg agcatcaaac cggcaacatg agttcccaag
      481 gcttgtctca aggcctcaac actcatcacg ccgctgctgt ctgacgggac agtctgaacg
      541 ctgtaacctg caatggccgc actggctggg ttggttccgt gggcactgtc gggaatcagg
      601 acagtggttt tcttgttgcc cttgtccttg tgataggcgg ccatgagcat aactcccgtc
      661 agttccccgt gtgccccggc gagaggttgc atcgtaaaag ccttcattcc tgtgatctca
      721 cgaagcaacc gatccatctc gtgtagcact tccaaagcac cctgaaccat catgtcccca
      781 ccgggaagct gaggcatcag cggatgcagg taggcaaacc ctggatactg cgcgatcttc
      841 tcggtgacct tcggattgta tttcatcgtg catgaaccca gaggatagaa accgttgtcc
      901 actccaaaat tccggcgact caactcggtg aaatgccgta caacatccaa ctcggacagt
      961 tcgggtaaag cgggtggctt ttcacgcaac agtgactctc gaatattggc cttctctcca
     1021 acgtcac
//
LOCUS       DAOQUC010001660         1026 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_220558_length_1026_cov_2.213182, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001660 DAOQUC010000000
VERSION     DAOQUC010001660.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1026
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_220558_length_1026_cov_2.213182"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1005)
                     /locus_tag="PKH07_20700"
     CDS             complement(<1..1005)
                     /locus_tag="PKH07_20700"
                     /inference="COORDINATES: protein motif:HMM:NF040068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HNT37426.1"
                     /translation="MLTFPDLYDIGTSHLGLKILYSILNGLHNVSCERAFAPWVDFEK
                     ELRVRGACLCSLESQQPLSSFDVVGFTLQHEMLYSNVINMLDLGGIPLHSRDRAEKDP
                     LILAGGPCAVNPEPLADFIDAFVIGDGETAVVTLSETLLQCKADALDRKATLDRLARI
                     PGVYVPQFYEPVWNADGTLSELAPVDPAYPNRVKSLKCTLRGLPYPVEQVIPLVDSVH
                     DRASIEIRRGCTRSCRFCQAGYISRPIRERDPDEIAYLSKKTLENTGFDEVSLMSLSS
                     GDYSQILPLLNVLQSELQSVRGHVVVPSLRLDRVPESFLNGLSSVKQTSLTFAPEAGS
                     S"
BASE COUNT          270 a          261 c          273 g          222 t
ORIGIN      
        1 cgagctgcca gcttccggag caaacgtaag gcttgtctgc ttgacagacg acaaaccgtt
       61 gagaaaagac tcggggacac ggtctagtcg gagcgacgga acgaccacat gccctcgaac
      121 tgactggagt tcagactgca gaacattcag aagaggaagg atttgggaat aatcaccgct
      181 ggagagcgac atcagagaga cttcatcaaa tcctgtattc tcaagtgttt ttttactcaa
      241 gtatgcaatt tcatcgggat cgcgttcccg aatcggtctg gaaatgtacc ctgcttgaca
      301 gaatcggcag ctgcgagtgc accctcttct gatttcgatg gatgctctgt cgtggactga
      361 gtccaccaag ggtataacct gttccacggg atagggaagg cctctgagtg tacattttag
      421 agacttgacg cggtttggat atgcgggatc aaccggtgcc agctcgctca acgtgccatc
      481 tgcattccag acaggctcat agaattgggg aacgtagacg cctggaatgc gcgcgagccg
      541 gtcgagagtc gcctttctgt caagcgcgtc ggccttacac tgaagaagtg tctctgacaa
      601 ggtgacgact gctgtctccc catcgccgat cacaaaggcg tcaatgaaat ccgccaaagg
      661 ctccggattc accgcacagg ggcctccagc gaggatcagg ggatccttct cggcgcgatc
      721 gcgggaatgc agaggaattc cacccaaatc caacatattg ataacgttgg agtaaagcat
      781 ctcatgttgg agtgtgaacc cgacaacgtc aaaactcgac agaggctgct gactctccag
      841 agaacacaga caggcacctc gaactcgtag ttctttctca aaatccaccc aaggggcaaa
      901 agcacgttca caggagacat tgtgaagtcc attcagaatg gaataaagaa tcttcagacc
      961 caaatggctc gttccgatgt cgtaaaggtc ggggaatgtc agcacccatc tcagtcttga
     1021 gtttgg
//
LOCUS       DAOQUC010001661         1025 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_220830_length_1025_cov_2.565979, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001661 DAOQUC010000000
VERSION     DAOQUC010001661.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1025
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_220830_length_1025_cov_2.565979"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            97..648
                     /locus_tag="PKH07_20705"
     CDS             97..648
                     /locus_tag="PKH07_20705"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNT37427.1"
                     /translation="MRDLSLHVLDLMENSVRAGATHIDLLCDIDTHVNRLVLRIQDNG
                     AGLLVSHEQALDPFYTTKDGKKTGLGLSLFQAAAEAANGNLELSPSPLGGLSVQATME
                     WDHLDRSPVGDLVESIFSLLCTEPDLDVTFQVRFDDRTFSVRVSEVVQTLSAEQRNSF
                     EALIHAKEALREVYRVIPELYGA"
     gene            complement(636..>1025)
                     /locus_tag="PKH07_20710"
     CDS             complement(636..>1025)
                     /locus_tag="PKH07_20710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37428.1"
                     /translation="HRTGITGVYAKDLTREGVNEALRSRRCFAATGVPIVVDFQANGH
                     IMGEEYETAVPPKFEVGIQGVSALQYVTLVRNNEDIFQHGGDSTDARFTYIDKDIAPG
                     ISYYYIRVLQRDNEMAWSSPIWVNYAP"
BASE COUNT          238 a          271 c          260 g          256 t
ORIGIN      
        1 acattggtcg ctctcgaact gttttgagga tggagaatcc gagtttcgag gagctgcgac
       61 tgtgtctcca tggagaacaa ggacggagcg ttcaacatgc gtgacctatc gctgcatgtt
      121 ctcgatctca tggaaaactc cgttcgcgct ggagccactc acatcgatct gctttgtgat
      181 attgacaccc acgtgaaccg tcttgtcctt cgcatccaag acaacggagc cggcctgctg
      241 gtgtctcacg aacaggcgtt ggatccgttc tacactacaa aggatggcaa gaaaaccgga
      301 cttgggctga gtctttttca ggctgctgca gaggcagcca acgggaatct ggaactgagt
      361 ccttctccac tgggagggct ttctgttcag gcgaccatgg agtgggatca tctggatcga
      421 agtcccgttg gagatttagt tgaatcgatc ttttcgctgc tgtgtaccga gccggatctg
      481 gatgtgacat ttcaggttcg attcgatgat cgaacctttt ccgtccgcgt ttcagaggtt
      541 gttcaaacgt tatcagccga gcaacggaat tccttcgaag cactgatcca cgcaaaagaa
      601 gcacttcgag aagtctacag agtcatccca gaactctacg gagcatagtt cacccagatg
      661 ggggatgacc atgccatctc gttgtccctc tgtaacaccc gaatgtagta atacgaaatc
      721 ccaggggcaa tatccttgtc aatgtatgta aacctggcat ctgtcgaatc accaccatgc
      781 tgaaagatgt cctcgttgtt gcgcaccagc gtgacatact gcaatgcgga tacgccttgg
      841 atgcccacct caaacttggg tggcactgct gtctcgtact cttcacccat gatatgtccg
      901 ttcgcctgaa agtctacaac aatgggaaca ccggttgccg cgaaacaacg ccgtgaacgc
      961 agggcttcat tcaccccttc gcgagtcagg tctttggcgt aaactccagt gattcccgtt
     1021 cgatg
//
LOCUS       DAOQUC010001662         1025 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_220914_length_1025_cov_2.136082, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001662 DAOQUC010000000
VERSION     DAOQUC010001662.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1025
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_220914_length_1025_cov_2.136082"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..993)
                     /locus_tag="PKH07_20715"
     CDS             complement(<1..993)
                     /locus_tag="PKH07_20715"
                     /inference="COORDINATES: protein motif:HMM:NF021351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HNT37429.1"
                     /translation="MYSVPALIVKCGANKERTRSEKEEDITNAIIKVTHTEEKSLYFL
                     TGHGERDINGETDAFGMGIIKRLLEDKRYSVKEFEMSRQQGIPEDCAVLVIAAPQTDL
                     FEHELTALERYLNLGGNVIVLLEPETAPQLAAWLAKYGLKVGNEIICEAEVQADIASL
                     LQGKLSGRLSVSTAPVIGKRNYGTHEITRDFDLATQYPSSRSVSVTDTLPAGITADKI
                     LETKGELENAPDASIPGSWAETDLEMLRQQQAQYDEGVDMKGPVTLAALVTIDRHNFE
                     PAKAAEAAVASATDIQRPDKSRLLVFGDSDFATNSGVRYDRGVLFTNCINYLIGA"
BASE COUNT          187 a          288 c          294 g          256 t
ORIGIN      
        1 ggctccgatg aggtagttga tgcagttggt gaacagcaca ccgcgatcgt agcgaacccc
       61 ggagttggtc gcaaaatccg aatctccgaa caccagaagt cggctcttgt ccggtctctg
      121 aatgtccgta gcagaagcga cggcggcctc tgcggccttg gcaggttcaa agttgtggcg
      181 gtcaatggtc accagtgccg caagcgtcac ggggcccttc atgtccaccc cttcatcata
      241 ctgagcctgc tgctgtctga gcatttccag atcggtctca gcccagctgc ctggaatgct
      301 cgcatccgga gcattctcca actcgccttt ggtttcgagt atcttgtcag cggtgatccc
      361 tgccggaagg gtgtccgtca cggacacgga gcggctggaa ggatactggg tggcgagatc
      421 gaaatcgcgt gtgatttcat gagtgccata gtttcgtttg ccaatgaccg gtgccgttga
      481 aacggagagc ctgcctgaca gtttgccctg aagcagggag gcgatatctg cctgaacttc
      541 ggcttcacaa atgatttcgt tgccgacttt caagccgtac ttggcaagcc atgctgccag
      601 ctgcggtgcg gtctcaggct ccaacaggac gatcacgttg ccacccaggt tcaggtatcg
      661 ctccagtgcg gtcaactcgt gttcgaacag atcggtctgt ggtgcggcga tgaccaagac
      721 ggcacagtct tctggaatcc cttgctgccg agacatctcg aattccttga ccgagtaacg
      781 tttgtcttcg agcagccgtt tgatgattcc catgccgaag gcatccgtct caccgttgat
      841 gtcccgctcg ccgtgtcccg tcaggaagta gaggctcttt tcctcggtgt gggtcacctt
      901 gatgatggcg ttggtgatgt cctcttcctt ctcgcttctg gttcgttcct tgttggctcc
      961 acatttgacg atgagcgcgg gaacggagta caactcgttg cggaactcct gcgccttgcc
     1021 gtgtt
//
LOCUS       DAOQUC010001663         1024 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_221227_length_1024_cov_2.474716, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001663 DAOQUC010000000
VERSION     DAOQUC010001663.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1024
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_221227_length_1024_cov_2.474716"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1005
                     /locus_tag="PKH07_20720"
     CDS             <1..1005
                     /locus_tag="PKH07_20720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNT37430.1"
                     /translation="CSLHCRHCRAAACADRNPLELDTESVRATIQSIARHYSPIIILT
                     GGEPLLRPDLFEIIQESVKAGLRTVMATCGVGLDESLAQRLKQAGIARLSFSLDGDDA
                     VTHDAFRQVPGAFEACLNAMRSAREVGLSFQVNTTVTRTNMSRLDQIWRKAVDLGAAA
                     FHPFLLVPTGRGKEIRDEVLSAAEYERVLHHIAQLSLKSPIPFKPTCAPHYCRILGQV
                     NHGVSALDSMTRGCLGGIGFAFISHIGKVQICGFLEEEAGDLRTTGFDFGQIWETSPL
                     FLSLRDKSNYRGKCSRCEFWHRCGGCRARAFAMTGDWLASEPCCDYIPAPETTRGTPA
                     "
BASE COUNT          225 a          261 c          303 g          235 t
ORIGIN      
        1 tgttcgttgc actgtcggca ttgccgtgcg gcagcctgtg cagatcgaaa cccattggaa
       61 ctggacacag agagtgtccg cgcgacgatc cagagtatcg ccagacatta cagcccgatt
      121 atcatcctta ccggaggtga gcctctcctg cgccccgatc tctttgagat cattcaggaa
      181 tccgtgaaag caggtctgag aacggtgatg gcaacctgtg gtgttggact ggatgaatcc
      241 cttgcacaac gtttgaaaca ggccggcatt gcgcgtctca gtttcagtct cgacggtgac
      301 gatgccgtga cgcatgacgc ttttcgacag gtgcctggag cttttgaagc gtgtctgaat
      361 gccatgcgaa gtgccagaga agtcggtttg tcttttcagg tcaacacaac tgttacccga
      421 accaacatga gtcgcctgga tcagatctgg cgaaaggcag ttgatctggg cgctgcagcg
      481 ttccatccgt ttcttctggt gccgactgga agaggaaagg aaatccgcga cgaagtgctg
      541 agtgcggcgg aatatgagcg tgtcctgcat catatcgccc agttgagcct gaagtccccc
      601 attccgttca agccaacctg tgcccctcac tattgccgga ttctcggcca ggtgaatcac
      661 ggagtgagcg cactcgacag catgacacgt ggatgcctgg gcggaatcgg atttgcattc
      721 atcagccaca tcggcaaggt gcagatatgt ggttttctgg aagaggaagc gggcgatctt
      781 cgcacgacgg ggttcgattt tggacaaata tgggagactt ctcccctgtt tctcagtctg
      841 cgggacaaga gtaactatcg agggaagtgt agccgatgcg agttctggca tcgctgtggc
      901 ggctgtcgag cgcgagcctt cgcaatgaca ggagactggc tggcatcgga gccctgctgc
      961 gattacattc cggcacctga gaccacgaga ggaacaccgg catgaaagag ttgaatggtt
     1021 ttct
//
LOCUS       DAOQUC010001664         1023 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_221577_length_1023_cov_2.827479, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001664 DAOQUC010000000
VERSION     DAOQUC010001664.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_221577_length_1023_cov_2.827479"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            411..>1023
                     /locus_tag="PKH07_20725"
     CDS             411..>1023
                     /locus_tag="PKH07_20725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37431.1"
                     /translation="MMNASQTTSSQLRNPLLNFIGEGIWGFQASFVASATVLTILLQD
                     FGASERLIGSITAIEGVLTSLPSVIGVYLFRSERTRKRGLLLWHAVAMVPFLFVSGAL
                     VSYSPHLSARVVRLGLLASFACFMASLGVIVGVWMDWLAHLYDIRIRGTVLGAGFFAS
                     ATMGSAGGLIAGWILRSYPGRTTFAGFYYLAGVLALLSILCFCF"
BASE COUNT          186 a          277 c          272 g          288 t
ORIGIN      
        1 agaacaattc cttttcccaa gacatattcc cacctctccc ttgaatgttt cctctcggcg
       61 gaaagttcaa ccctccgata tgtgtacccc gacatatccc cacgagaaac gcactcgatt
      121 tctcagagca atccctatat accatgatcg gtttggctgg gcaagtcttt gaagtcaaac
      181 gggtttgcct ctgagcaggt tggcacctgc gggcgtgcgg gccatgccaa agcctgtgtc
      241 gtgatagctc atgaaggaac tcaccgctct tggagtcgag ctcacccacg ggaagaggcg
      301 tatgccttga cgtccgctgt ggttcatgtg acgatccagg aacagattca cctgagcgca
      361 ggcacgggag attcacagat gtataatgga caacggacgg gactcctttg gtgatgaacg
      421 catcccagac aacttcttcc caactacgaa atcctctgct gaacttcatt ggcgaaggca
      481 tctggggatt tcaggcttct tttgttgctt cggcaaccgt gttgactatt ttgcttcagg
      541 atttcggcgc gagtgagagg ctcatcggat ccatcaccgc tatcgagggc gttctcacct
      601 cactccccag cgtcatcggt gtgtatctct ttcgctctga gcgcacacgc aaacgcggtc
      661 tcctgctctg gcatgcggtg gcaatggttc cttttctgtt tgtcagtggc gcactggttt
      721 cttactcgcc ccatctgagt gcgcgtgttg tgcgcttggg tcttctggct tcctttgcgt
      781 gttttatggc atccttgggg gtgattgttg gcgtctggat ggactggctc gctcatcttt
      841 acgacatccg gattcgggga acagttctgg gtgccgggtt tttcgcctca gcaacaatgg
      901 gatcggcagg tggcttgatc gctggatgga ttctgagatc gtatcccggg cggacaacct
      961 tcgctggctt ttactacctt gcgggagttc ttgcgctcct gtccattctt tgtttctgct
     1021 tca
//
LOCUS       DAOQUC010001665         1023 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_221620_length_1023_cov_2.517562, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001665 DAOQUC010000000
VERSION     DAOQUC010001665.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_221620_length_1023_cov_2.517562"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1023
                     /locus_tag="PKH07_20730"
     CDS             <1..>1023
                     /locus_tag="PKH07_20730"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013885896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HNT37432.1"
                     /translation="FEAVHAVAVQIEGPIIAGLSRCMEKDIQCCWDAVKPSKHPRIHT
                     FIATSDIHMEKKLRKPPEAVLQMAIEGTKFARGLCEDVEFSCEDSTRTRPEFLAKVVQ
                     AVIEAGASTVNIPDTVGYTNPWEYERLIRYLFEHVKNIEKAIISVHCHNDLGLGVPNS
                     LAAIIAGARQVECTVNGLGERAGNASLEEIVMNLKVRADFFKIPTTVDTTRLYPTSRL
                     VSNLTGIAVQRNKAIVGQNAFAHEAGIHQDGMLKERETYEIMRPEDVGVPGSSMVLGK
                     HSGRNAFRTRLKDLGYDLDDEMLNKAFEQFKVLADKKKDIFDEDLQVIVEQEARASAE
                     IFHLDYV"
BASE COUNT          245 a          270 c          297 g          211 t
ORIGIN      
        1 tttcgaggca gtccacgccg tggcggtgca gatcgaaggc cccatcatcg caggtctttc
       61 ccgctgcatg gagaaagaca ttcagtgctg ctgggatgcc gtaaagcctt ccaagcatcc
      121 gcgcatccac accttcatcg cgaccagtga catccacatg gagaagaaac tgcgcaagcc
      181 gcctgaagcc gtgctgcaaa tggccatcga gggcaccaaa ttcgcccgag gcctttgtga
      241 ggatgtggag ttttcttgtg aggactctac gcgaacacgt ccggagtttc tggccaaggt
      301 ggtgcaggca gtcatcgagg caggtgcctc gacagtgaac attcccgata cggtggggta
      361 caccaacccg tgggagtatg agcgactgat tcgctatctg tttgagcatg tcaagaacat
      421 tgaaaaggcg atcatcagtg ttcactgcca taacgatctg ggcttggggg ttccgaactc
      481 cctggcagcc atcatcgcgg gggcccgaca ggtggaatgc actgtcaacg gtctcggaga
      541 gcgcgccggc aacgcgtctc tggaagagat cgtcatgaat ctgaaggttc gagccgattt
      601 cttcaagatt cccacgacgg tggacacgac tcgtctctat ccgacaagcc gcttggtgag
      661 caacctgacc ggtattgcgg ttcagcgcaa caaggccatc gtcggccaga atgccttcgc
      721 tcatgaagcg ggcattcatc aggacggcat gctcaaagaa cgagaaacct atgagatcat
      781 gcgtcctgag gatgtcggtg ttccaggctc atccatggtt ctgggcaagc actccggtcg
      841 aaacgcgttt cggacgcgcc tgaaagacct gggttacgac ctggacgatg agatgttgaa
      901 caaagcgttc gaacagttta aggtactggc agacaaaaag aaggatatct tcgatgaaga
      961 ccttcaagtg atcgtagagc aggaagcccg cgcgagtgcg gaaatcttcc atctggacta
     1021 cgt
//
LOCUS       DAOQUC010001666         1023 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_221643_length_1023_cov_2.442149, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001666 DAOQUC010000000
VERSION     DAOQUC010001666.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_221643_length_1023_cov_2.442149"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1023
                     /locus_tag="PKH07_20735"
     CDS             <1..>1023
                     /locus_tag="PKH07_20735"
                     /inference="COORDINATES: protein motif:HMM:NF020926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1998 domain-containing protein"
                     /protein_id="HNT37433.1"
                     /translation="DQFIVQHPEYFFGSTPESGIVNPNNLAILISHLKCAAFELPFDD
                     HEEFGMKDISPVLGFLEEEFVLRHRGNRWYWSSEVYPAEEISLRRATMENFIILNVSN
                     KNQTLGEVDRPSAPLLIHPNAVYMHQSETFLIENLDWDNRIAYARKSDVDYYTDAIAK
                     MDLRVLRVEDRIDFTPPEGEPPLKTLLPSPLERPLPEPGQEYPLVAGQFDQDQSIAPE
                     QPGRKFGEVAVTILATKFKKIKFETHESVGYGEIHLPEENLQTEAYWVDLPMDLVAGL
                     EERDLGGALYGLATALGSVVPVYVLCDPKDLRTVPQVRSPFSNLPALFVFDNYPGGIG
                     IASKVYD"
BASE COUNT          231 a          261 c          280 g          251 t
ORIGIN      
        1 gaccagttta tcgttcaaca tcccgaatac ttctttggca gcacacccga aagtggcatc
       61 gtgaatccca acaatctggc gattctcatc agtcatctga aatgcgccgc cttcgaattg
      121 ccttttgacg atcatgagga attcgggatg aaggacatct ccccggtgct cggatttctc
      181 gaagaggagt ttgtcctgcg ccatcgggga aatcgttggt actggtcgag cgaagtctat
      241 cctgcggaag agatcagcct tcgacgtgcc acgatggaga acttcatcat cctgaatgtt
      301 tccaacaaga atcagacatt gggcgaggta gaccgaccca gcgcgccgct tctgattcat
      361 cccaacgcag tctacatgca tcagagcgag acttttctca tcgaaaacct cgactgggac
      421 aatcgaattg cctacgcgag aaagtcggac gtggactact atacggatgc cattgcaaaa
      481 atggatttgc gggtgttgcg ggtggaagat cgaattgact ttactccccc tgaaggcgag
      541 ccgcctttga agacgctgct ccccagtccc ttggagcgtc cgcttcccga accagggcag
      601 gagtatcccc ttgtggcggg tcagttcgac caggatcaat ctattgcgcc ggaacaacca
      661 ggtcggaaat tcggtgaggt ggcggtgacg atccttgcca ccaagttcaa gaagatcaag
      721 tttgagactc acgaatccgt tggctacggc gagattcatc tgcctgaaga gaatcttcag
      781 acggaagcct attgggtgga tctgcccatg gacttggttg ccggactgga agagagagac
      841 ttgggtggag cgctctatgg tttggcgaca gcgcttggca gtgttgttcc ggtctatgtg
      901 ctctgcgatc cgaaggatct gcgtacggtg cctcaggttc gttctccgtt ttcgaatctt
      961 ccggcactgt tcgtatttga caactaccct ggtggaattg gtattgcctc caaggtctat
     1021 gac
//
LOCUS       DAOQUC010001667         1022 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_221930_length_1022_cov_4.217166, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001667 DAOQUC010000000
VERSION     DAOQUC010001667.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1022
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_221930_length_1022_cov_4.217166"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..414
                     /locus_tag="PKH07_20740"
     CDS             <1..414
                     /locus_tag="PKH07_20740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37434.1"
                     /translation="DLVQHFGSRTDLAANTYCVTRCSVGHPWQAPVITCFADYFHLSP
                     CGKKAIDAHEQVHVRQFLDRCSSYMDCLALSRFFADSLECEAWMDTETAIKRLQKDPC
                     CQSSRDQQDLRQLLRATIHENIPYFCGWRKIVPIL"
     gene            426..788
                     /locus_tag="PKH07_20745"
     CDS             426..788
                     /locus_tag="PKH07_20745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37435.1"
                     /translation="MFILFSFLVVLWRIILLFLGVWTFPRLLTKTEPTKASTAGILHG
                     FIFYGLPLLLEPATTPPQFTVLLPLLGVLIYSPLSGIVLRLLRFGTFWEGMKTSVYTI
                     GIGSCIAAALEVAVRVIP"
BASE COUNT          210 a          256 c          261 g          295 t
ORIGIN      
        1 gacttggttc agcactttgg atcgaggaca gatcttgctg ctaatactta ctgtgtcact
       61 agatgctccg tcggacaccc atggcaagct cctgtgataa cctgttttgc ggactacttt
      121 catctctccc cgtgcggcaa gaaggctatt gatgcacatg agcaggttca cgtcaggcag
      181 tttctcgaca ggtgcagtag ctatatggac tgtctagcct tgagcaggtt ctttgctgat
      241 tcacttgagt gtgaggcgtg gatggatacg gagacagcga tcaagaggtt gcaaaaagac
      301 ccgtgttgcc aaagcagcag ggaccagcaa gacttgaggc aactgctccg cgccacgatt
      361 catgagaaca ttccctactt ctgtggctgg cgcaagatcg tcccgatcct ctaggagcac
      421 ttctcatgtt cattctcttt tctttcttgg ttgttctatg gagaatcatt ctgctgtttc
      481 ttggagtctg gacgtttcct cgtcttctca ccaagactga accgacaaag gctagcactg
      541 ctggcatcct gcacgggttc atcttctatg gcttgccctt gctgttggaa cccgctacga
      601 cgccacctca gtttactgta cttctcccgc tcctgggtgt actcatctac tcgccacttt
      661 ctgggattgt tcttcggttg ttgcgattcg gtaccttctg ggagggtatg aagacttcag
      721 tatatactat tggtattggc tcgtgcattg cggctgcact agaagtcgca gtgcgtgtca
      781 taccatgata tctttgactg ccatggttga tctatggcag caagcgaagt cgttttggcg
      841 accgaatgcg ttccactttg gaagcaacga gcctatcgag aaggagtatt ctgtatctat
      901 gatgtcttca tcgcttcaat atgagtctga agacgtgata gctacgcttg attcctatgg
      961 gaatacaact cattggcagc aagacgcctt cgggcgggct gtgcccgcgt ccacgccttg
     1021 gg
//
LOCUS       DAOQUC010001668         1022 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_221951_length_1022_cov_3.282316, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001668 DAOQUC010000000
VERSION     DAOQUC010001668.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1022
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_221951_length_1022_cov_3.282316"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..316
                     /locus_tag="PKH07_20750"
     CDS             <1..316
                     /locus_tag="PKH07_20750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009438832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HNT37436.1"
                     /translation="NPTHYAVALAYDRAVMLAPTVLAKGRGIIAQRIRQIAEESGIPI
                     VEDPELARALYHSVEIGMRIPEVLYQAVARVLAYVYRMRERHKRRLPARKASAPLVGA
                     SG"
     gene            442..>713
                     /locus_tag="PKH07_20755"
     CDS             442..>713
                     /locus_tag="PKH07_20755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
                     /protein_id="HNT37437.1"
                     /translation="MADATGKKSRLGGPTDLLVAVGILGILGILVIAMPPSLLSLLLI
                     LNLAISLLILLVSFYILEPLQFSTFPALLLIVTMFRLAMNVASTKL"
     assembly_gap    714..723
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <724..>1022
                     /locus_tag="PKH07_20760"
     CDS             <724..>1022
                     /locus_tag="PKH07_20760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
                     /protein_id="HNT37438.1"
                     /translation="ILGESQVGGDLKGKAGAVIQFFGQVVARDDPVVGFVVFLIMVLI
                     NFIVITKGAGRIAEVAARFTLDAMPGKQMAIDADLNAGLITEQQARDRRSTISREA"
BASE COUNT          204 a          264 c          304 g          240 t
ORIGIN      
        1 caacccgacg cactacgcgg ttgcgcttgc ttacgatcgt gccgtcatgc tggcaccaac
       61 ggtactggct aaaggcagag ggatcatcgc tcagcgaatt cgtcagatcg ccgaggaaag
      121 tgggattccc atcgtggaag atcccgaact ggcgcgagcg ttgtatcatt cggttgagat
      181 cggaatgcgg attcctgagg ttctctatca ggcagttgcc cgtgttctgg cttacgtgta
      241 tcgaatgcgg gagcgacaca aacgaagact tccggccaga aaggcgtcgg ctccattggt
      301 gggagcgtca ggatagtgag caggagacag ctgggagtga atcggcgctc ttgacgcggg
      361 tgtccgaaaa agaaaaagac cttcccccgc cgtccttggt aaggacgggg aatgtgaggg
      421 gatggtttct cagaggcaaa gatggcagac gcgacaggca aaaagtccag actcggcggt
      481 cccacggatc ttctcgtggc ggtcgggatt ctgggtattc tgggcatact ggtcatcgca
      541 atgccccctt cgctgctgtc gctgctcctg atattgaacc ttgctatctc actgctgatt
      601 ctccttgttt cattctacat tctcgagccg cttcagttct ccacatttcc tgccctgctg
      661 ctcattgtga cgatgtttcg gctggcgatg aatgttgcct cgaccaagtt gatnnnnnnn
      721 nnnattcttg gagagtctca ggtgggtggc gatctgaaag gcaaggccgg agcagtcatc
      781 cagttctttg gtcaggtggt ggcgcgagac gatccggtcg ttggttttgt ggtctttctg
      841 atcatggtgc tcatcaactt catcgtcatc acaaagggtg caggccgaat cgcagaagtc
      901 gcggcgcgct tcaccctgga tgccatgcct ggcaagcaga tggccattga cgcggatctg
      961 aacgctggtc tcatcaccga acaacaggcg cgcgatcggc gttcgaccat ctcccgtgag
     1021 gc
//
LOCUS       DAOQUC010001669         1022 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_221964_length_1022_cov_3.136505, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001669 DAOQUC010000000
VERSION     DAOQUC010001669.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1022
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_221964_length_1022_cov_3.136505"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..259
                     /locus_tag="PKH07_20765"
     CDS             <1..259
                     /locus_tag="PKH07_20765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37439.1"
                     /translation="HGTNDVPAMGNVWDSISPETIEAMIYHKPDDQTDADRNGSLSGE
                     VFYIPYSKMPETPGHVDVNWSGRVDLDDLLLIQQNWHQMQE"
     gene            complement(410..>1022)
                     /locus_tag="PKH07_20770"
     CDS             complement(410..>1022)
                     /locus_tag="PKH07_20770"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNT37440.1"
                     /translation="LLGRFGYSLRSPNFRAFLIGRILAVVGFCILPFITVYYRSTLGG
                     ALPEHQLIWCSTAMTAATALTFLFLGLLGDRRGHRIGVIVGTLAQVASLSVLLMSSGL
                     WSCMAAYFCAGIAIGAGFLSHVNMLFETCPHDHRLSHITVANLVLAMPLLTAPLLAGV
                     LAEWAGLRTLFGVCLVFSVLSALWLTFRVRDPRHIEAYGIPSL"
BASE COUNT          297 a          274 c          280 g          171 t
ORIGIN      
        1 ccacggaacg aatgatgttc cggcgatggg aaatgtctgg gacagtatca gtcctgagac
       61 aatcgaggcc atgatctacc acaaacctga cgatcaaact gacgcagacc gcaatggtag
      121 tctgtctggt gaggtcttct acattcccta ctcgaagatg cccgaaaccc ctggccacgt
      181 tgacgtgaac tggagtggca gagtggatct ggatgatttg ctcctgattc aacaaaactg
      241 gcaccagatg caggaatgac ctgaaaggtg catgtgtcat cgcgacgccg aaaggccttt
      301 ccggaatatg ccctcacagg aacacgacaa acagaagaac ctagagggac gccctcagtc
      361 acagaggttt cttcgcttga atactcagaa agacaatgtg cgtcctgcat cagagcgacg
      421 gaatgccgta ggcctcaatg tgcctggggt cgcgcacacg gaaagtcaac cagagagccg
      481 acaacacgct gaagacaaga catacgccaa agagtgtccg aaggcctgcc cactccgcca
      541 agactcccgc cagcagaggt gctgtgagaa gaggcatggc caaaaccaag ttggcaacag
      601 tgatatggga cagacgatga tcgtggggac aagtctcgaa aagcatgttg acgtgcgaca
      661 ggaaacctgc cccaatggca atccccgcgc agaagtaggc ggccatacaa ctccaaagtc
      721 cgctgctcat caacagaacc gagagagagg caacttgtgc gagcgttcca acgatgactc
      781 caatgcgatg gcctcgcctg tctcccaaga ggccaagaaa cagaaaggtc aacgccgttg
      841 ccgcggtcat ggcagtgctg caccagatca gttggtgctc gggcaaagcg ccaccaaggg
      901 ttgagcggta atagaccgta atgaaaggca gtatacagaa cccgacaacc gcgagaatac
      961 gaccgatcaa gaaggctcga aaattggggc ttctcaggct gtatccaaaa cgccccaaga
     1021 ga
//
LOCUS       DAOQUC010001670         1020 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_222783_length_1020_cov_2.524352, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001670 DAOQUC010000000
VERSION     DAOQUC010001670.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1020
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_222783_length_1020_cov_2.524352"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="PKH07_20775"
     CDS             <1..288
                     /locus_tag="PKH07_20775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNT37441.1"
                     /translation="ETEVTLRTSWSPAQTLNPGDYDAVFREHAVILAAGRYQLVIGLS
                     SYERTFHYVRNAGILEVAGFAEGIDLVRVSNTALVLNPMTVDIMPTKGGSL"
     gene            285..986
                     /locus_tag="PKH07_20780"
     CDS             285..986
                     /locus_tag="PKH07_20780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018284471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HNT37442.1"
                     /translation="MRFARLKRYLPWGKPRFYRRSYAQSGEDVILDYLLNWIGVAKPS
                     YLDIGAHHPKHLSNTFFFYQKGCHGVVVEPDPILYALIKKVRNRDICLHCGVGGEDQE
                     DAEFYVMNPQTLNTFSRKEMEEFVASGKYSLQETLRIPMLSVNTIIERYFDSAPDILS
                     LDVEGLDWQILQSLDFTRFRPRTVCVETVTHIQQTKIQEILDWMCDRGYTVYADTFIN
                     TIFVERQAWAGRRRG"
BASE COUNT          238 a          253 c          276 g          253 t
ORIGIN      
        1 gagaccgaag tgacgttgcg cacttcatgg agccccgccc agacgctcaa cccgggtgat
       61 tacgatgcgg tgttccgtga gcatgctgtc atccttgccg ctgggcgtta ccagctggtc
      121 atcgggctgt cctcctatga gcgtacgttc cactacgttc gcaatgcggg cattcttgaa
      181 gtggccggat ttgccgaagg aatagacctt gtccgagtga gtaacaccgc cttggttctg
      241 aatccgatga ccgttgatat aatgccgacg aagggggggt ctctgtgagg tttgccaggc
      301 tcaagagata tcttccctgg gggaaaccca ggttctacag acgcagttat gcacagagcg
      361 gcgaagacgt catccttgac tacctcctga actggattgg tgttgccaag ccctcatacc
      421 tggacattgg tgctcaccat ccaaaacacc tgagcaacac gttcttcttc tatcagaagg
      481 gatgccatgg tgttgttgtc gaaccggatc ccatactgta tgccctcatc aaaaaggtcc
      541 gcaatcgcga tatctgcctg cattgcggtg tcggtggtga agaccaagag gatgccgagt
      601 tctacgtgat gaaccctcaa acgctcaaca ctttttcgcg gaaagagatg gaggaatttg
      661 tcgcctctgg aaagtacagt cttcaggaga cgcttcgtat tccgatgctc tcggtcaaca
      721 cgatcatcga gaggtacttt gatagcgctc cggatattct ttctctcgat gtggaagggt
      781 tggattggca aatacttcaa tctctcgatt tcacgcgttt tcgtcctcga acagtctgcg
      841 ttgagacggt gacacacatt caacagacta agattcaaga gattcttgac tggatgtgcg
      901 accggggtta caccgtttat gctgatactt tcatcaacac aatcttcgtt gagagacagg
      961 cttgggctgg ccgacgaaga ggatagcaag accatgaaga tgagggcgat ggtgcagaga
//
LOCUS       DAOQUC010001671         1019 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_223174_length_1019_cov_2.324689, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001671 DAOQUC010000000
VERSION     DAOQUC010001671.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1019
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_223174_length_1019_cov_2.324689"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="PKH07_20785"
     CDS             complement(<1..202)
                     /locus_tag="PKH07_20785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37443.1"
                     /translation="MLILRILILLSCSSLCVLLMVWHSAQYRGKQTTLFFFTTCTVYA
                     ILRGHAVRYITTQQWGRAAPYTF"
     gene            complement(252..>1019)
                     /locus_tag="PKH07_20790"
     CDS             complement(252..>1019)
                     /locus_tag="PKH07_20790"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNT37444.1"
                     /translation="RGRCFSPPELDALSSLLVGNPRYENKSSRDLIFRLRRALTAFEK
                     PEFKSFLEHPLYLSAATMATDWYLARQYCHAKASGKGAPTHQQLQDYYSQYANTYSTK
                     PARRATVLLLPITSVSTSSPVDIHYTTQKNLEKALAIVEKARQGTPLAELARGQGVAG
                     DVSEVTSVWLQEPSFYEADTTLAKMQPGEISDPVRSSNGHYIFQLHETRPSEPIPFDE
                     VRDRVLSDYLWAREIEAWDELLLQAEESLDLKPCESQ"
BASE COUNT          226 a          250 c          291 g          252 t
ORIGIN      
        1 cgaaagtgta cggggcggct cttccccatt gctgcgtggt gatgtaacgg acggcatgcc
       61 ctctgagaat ggcgtagacg gtgcaggtgg tgaagaagaa aagtgtggtt tgtttccctc
      121 gatattgtgc cgaatgccag accatcagaa gtacgcagag cgatgaacat gacaggagaa
      181 tcagtatacg aagtatcaac atgattcaga tatctcccat cttgttttcg gcgtcagagc
      241 ctaattgctg actattgcga ttcgcagggc ttgagatcaa gggactcttc cgcttgcagg
      301 agcagttcat cccacgcttc gatctcccgc gcccagaggt aatcagaaag aactctgtcc
      361 cgaacctcat caaaggggat cggttcagag ggacgagttt cgtgaagttg gaaaatatag
      421 tgaccgttgc tggaacgaac cggatcactt atctcgcctg gttgcatctt tgccagcgtt
      481 gtgtctgctt cgtaaaagga gggttcctgg agccaaaccg atgtgacttc agatacgtca
      541 ccagccactc cctggcctct tgcgagttca gcgagaggtg ttccctgacg cgctttctcg
      601 acgatagcca gagccttttc cagattcttc tgagtggtgt agtgaatgtc cacggggctt
      661 gacgtggaaa ccgatgtgat cggcaacaag agcacggttg ctctgcgtgc cggctttgtg
      721 gaataggtgt tggcgtattg actgtagtag tcctgaagtt gctggtgcgt gggtgctcct
      781 ttcccggaag ccttggcgtg acaatactgc ctggcaagat accagtccgt cgccattgtc
      841 gccgcactta gatagagcgg atgctccagg aagcttttga actcaggctt ctcaaaggcg
      901 gtcagggctc ttcgaagcct gaagatcaga tcgcgtgaag acttgttttc atacctggga
      961 ttgcctacca gaaggctgct cagtgcgtcc agctcaggcg ggctaaaaca tcgccctcg
//
LOCUS       DAOQUC010001672         1019 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_223263_length_1019_cov_1.946058, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001672 DAOQUC010000000
VERSION     DAOQUC010001672.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1019
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_223263_length_1019_cov_1.946058"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1019)
                     /locus_tag="PKH07_20795"
     CDS             complement(<1..>1019)
                     /locus_tag="PKH07_20795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37445.1"
                     /translation="FNPIMPCIGSAVLNVNGPVLSGSGGLHVSVALRYHSVNDVTMSG
                     DFFGVSETQENSKWALSVDAFLQRNWDNNDLVFYRGGEYERSMRYEDGIWYDEVHCAT
                     ADLIWSSGKAYYRLWMPSGARIRYASDTGRIDRHISSFGTRVDYVYDTKEDRGLLVEI
                     KMPSIDGRSVQFQYDEAKPTRLISIVYPDSGVLSLEYDSVSGVLDGVFLYRGVRLWDN
                     DFEYAYGLMDKVYSYDMSSQPPTRHLIADYEHQYDQEEEEWVVTGMKKAGDSAYLQEV
                     QNEIEDASPGSFRRIRPDGSIYEVKITADGEDQVTRRRFYDAQETPVGPVSYQRQEPV
                     YHTIQ"
BASE COUNT          246 a          289 c          243 g          241 t
ORIGIN      
        1 ctggatggtg tggtacaccg gctcctggcg ctgataggac acgggaccga ctggcgtctc
       61 ctgagcgtca tagaatcttc gtcttgtcac ctggtcttca ccgtcggcgg tgatcttgac
      121 ttcgtagatc gatccgtcgg gtcttatcct acggaacgaa cctggagagg catcctcgat
      181 ctcattctga acttcttgaa gatatgcgga gtcaccggct ttcttcatgc cagtgacaac
      241 ccattcttcc tcttcctgat cgtactggtg ttcgtagtcg gcgatcaggt gccgagtcgg
      301 gggctggctc gacatatcat aggaatagac tttgtccatg agcccgtatg cgtactcaaa
      361 gtcattgtcc cagaggcgca caccacgata caagaacacg ccatccaaaa cccccgaaac
      421 gctgtcgtat tccagagaga gtaccccact gtcggggtac acgatggaaa tcagacgagt
      481 tggcttggcc tcgtcatatt gaaactggac gctccttcca tcaatagatg gcatcttgat
      541 ctcaaccagc agaccgcgat cttctttggt gtcgtagaca taatctactc gtgtgccaaa
      601 ggaactgatg tgacggtcta ttcgacctgt atccgaagcg tatcgaatcc tcgccccaga
      661 aggcatccaa agccgataat aggcttttcc agaagaccag atcaggtctg ccgttgcaca
      721 gtgtacttca tcataccaaa ttccatcctc ataccgcatg gagcgttcgt actccccacc
      781 ccgatagaag acaaggtcgt tgttgtccca attcctttgc agaaaggcgt ccacagatag
      841 tgcccacttc gaattctcct gtgtctctga aacgccaaag aagtcaccgg acatggtcac
      901 gtcattcacg gaatgatacc tgagagcaac gctgacgtgc agcccacccg accccgacaa
      961 tacaggaccg ttgacattca gaaccgcgct cccgatacag ggcataattg ggttaaaga
//
LOCUS       DAOQUC010001673         1019 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_223340_length_1019_cov_1.544606, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001673 DAOQUC010000000
VERSION     DAOQUC010001673.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1019
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_223340_length_1019_cov_1.544606"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            571..1008
                     /locus_tag="PKH07_20800"
     CDS             571..1008
                     /locus_tag="PKH07_20800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37446.1"
                     /translation="MKNCITFSVLFGVMLVASTALAQFNETWESYGTISEAVNSGGWV
                     RQGVSSHTIDSVTTASLPRPGAPEPTNKVLRAYNGDENLVHTFDPRIGTDASPLIVQV
                     WMYDLGGSSTKGVSLIRTLDGGLSSSFHCGIHRQDSGWRRIPQ"
BASE COUNT          252 a          245 c          264 g          258 t
ORIGIN      
        1 ccttcgtaag agttcacgct ttagcgtgtc tttttccgga gcaacctgaa ggtcgaactc
       61 ttacgaagag cccatcgccc aaaggtttgt agtgtgcccg taagggcata tccggatcac
      121 ccctgacggg gtaactacaa acaggaaaag atcacgcttt atggcgtaac gacgaacgaa
      181 atcgcggcat gaatgccgca ctacaactct tgctactacg gctgtgatct tggttttttg
      241 tcaaacaatt cggagaggct atatctgagg cgatcgcaga gaacttcgag cgtttccaga
      301 gtaacgttct gctcaccgat ctcgattctg ttcagggaag acttcgatat gcccagtttc
      361 cgggcgaaat gcacctgtgg catgtcccct cgaatctctc tgagtctttg acccaataga
      421 agcgccaatc ttcccatttg tgggaaaaaa tacttgatct tatttcccac atgtgggata
      481 ttccaaaatg acggtttctg tcgatctgtg atgctgaaat atgatctctt tagcggtggg
      541 aaatccagag cggattcagg aggatttgag atgaaaaact gtatcacttt tagcgtctta
      601 tttggggtta tgctcgttgc aagcaccgcg cttgcgcagt tcaatgagac ttgggaatcc
      661 tatggaacga tttcagaagc ggtcaactcg ggggggtggg taagacaggg agtatcgagt
      721 cacacaattg actcggtaac aacagcgtct ctaccccgcc ccggagcgcc agaaccgaca
      781 aacaaggttc ttcgtgctta caacggtgac gagaatcttg ttcacacctt tgatcccagg
      841 ataggaaccg atgccagtcc gctgatcgtc caagtgtgga tgtacgatct gggtggatct
      901 tccaccaagg gagtcagtct gattcgaaca ctggacggcg ggctgtcgag cagttttcat
      961 tgcggtattc accgccagga ttccggttgg cggcgaatac cgcaatgaaa actgctcga
//
LOCUS       DAOQUC010001674         1018 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_223431_length_1018_cov_3.140187, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001674 DAOQUC010000000
VERSION     DAOQUC010001674.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1018
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_223431_length_1018_cov_3.140187"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..548)
                     /locus_tag="PKH07_20805"
     CDS             complement(<1..548)
                     /locus_tag="PKH07_20805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015337784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNT37447.1"
                     /translation="MTLSPEEACHFAEQASKGVGIAPAPAGASKHTIGSEPGIGRWAT
                     MEQNVPHKDGANSKTQVMRLTEIGIALSAELNLDALLEKIVHHARELTDADAGTLYLL
                     AGDCLHFKILQNASLDIFQGGTTGRQIDLKPVPLDRANVSAYAALEQKTVNIEDVYES
                     EEFDFSGPRRYDSVTGYRSKSML"
BASE COUNT          236 a          279 c          255 g          248 t
ORIGIN      
        1 agcatggact tcgagcggta tcccgtcaca ctgtcatacc gcctgggacc cgagaagtcg
       61 aattcctctg actcatagac atcctcgatg ttgacggtct tctgttccag cgcggcatag
      121 gcggagacat tcgcccgatc cagaggaacc ggtttgagat caatctgacg ccctgtcgta
      181 ccgccttgaa agatatctag acttgcgttt tggagaattt tgaaatgaag acagtcgcca
      241 gccagaagat acaatgttcc cgcatccgca tcggtcaact cacgagcgtg gtggacgatc
      301 ttctcaagca atgcatcaag attcagctcg gcagacagag caatgcctat ctccgtcaaa
      361 cgcatgactt gagtcttgga attggctcca tccttatgag gcacattttg ctccatggtc
      421 gcccaccttc cgattccagg ctccgagcct atagtatgct tgctagcacc tgcaggcgcg
      481 ggggcaatgc ccactccctt ggatgcttgc tcggcgaaat gacacgcctc ttctggactg
      541 agtgtcatta ggatttagat tcgttggctc acctcataaa gtcaagatga ttcttttcgt
      601 cagacccttg ccgcaaatag ccaagagtgc catgatagga gatactcagc ggaaacgagt
      661 cgccggaatc cagaatcagg gcggaggctg agtggtatca ggaggtgttc gtgtgcacag
      721 tgcccgtctc tcttgcacat ttcggatcgt gcagtgtggt ctcattcttc tacttctcat
      781 cccctctgtc cctgaagccg caggcatgtt ctctcgcgga cgggagccgt ctgtctcttc
      841 gatcaaggag actcatacgg tgaaagcaaa gaccattgac ttctacagtg gcaatctgag
      901 cgtcggctac ttcatgcact actggggcac ggcaaagcaa gagccattcg atcgtctcaa
      961 gcacgaacag gtcatcctga atcaatctct gatcgaattg ctccagagaa atcgctgc
//
LOCUS       DAOQUC010001675         1017 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_223856_length_1017_cov_2.641372, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001675 DAOQUC010000000
VERSION     DAOQUC010001675.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1017
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_223856_length_1017_cov_2.641372"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(124..330)
                     /locus_tag="PKH07_20810"
     CDS             complement(124..330)
                     /locus_tag="PKH07_20810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37448.1"
                     /translation="MSERICCANCAFSDEPFHDESSHEHKAGLICRRNAPEPLLRVRR
                     TEKQVHVQWPEVRPDDWCGEFKLR"
     gene            complement(453..>1017)
                     /locus_tag="PKH07_20815"
     CDS             complement(453..>1017)
                     /locus_tag="PKH07_20815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37449.1"
                     /translation="TWNWGGFRNCQAFTSVFPSPRISCIVTAAPDVVKMAFLDNLYLN
                     VMPRKTESVNGSDWIYNYPAMSEALKECAALRSQFLEYFTEGRFIGDNLLSESCPNAH
                     VAAYIRPNKALLLLLNRAGKRSVEFKCNLAPWIDSKDGKYIVKGFDSNGKQTQTRHIE
                     SASWTDEVSSMEPLGIALFEISSDGNR"
BASE COUNT          245 a          275 c          254 g          243 t
ORIGIN      
        1 ctctgattat ccacagatag cacagataga cacagattgt ttcctgtctt ccgtcctatc
       61 agcggtagtc tgcggaatct cgctttctga gaccgtcagc ccggaaggca agagaagacc
      121 tgttcagcga agtttgaact ccccacacca gtcgtcggga cgcacttcag gccattggac
      181 atggacttgc ttttcagtac ggcggactcg gagcaggggt tcaggagcat tgcgtcggca
      241 gatcagtccc gctttgtgct cgtgcgagga ctcatcatga aaaggctcat ccgaaaaagc
      301 acagttggca caacaaatcc gctccgacat gacgattctc ccttagtgca tgatgccgcc
      361 ttacacctga actctcattg cccaggaaag actccacaca atgagactgc aagacaactc
      421 ttggatcttg atacttcccg caactctgaa tctcaacggt tcccgtcaga actgatctcg
      481 aacaaggcga ttccaagagg ctccattgag gaaacttcgt cagtccaact cgcactctcg
      541 atgtggcgcg tctgagtctg tttgccgttg gagtcaaatc ccttcacgat gtacttgcca
      601 tctttcgaat ctatccacgg agcgagattg catttgaact cgacagagcg tttgcccgct
      661 cgattgagaa gtagcaacaa cgctttgttc ggtctgatgt acgctgccac atgagcgttt
      721 ggacacgatt cgctgagcaa attgtcaccg atgaaacgcc cttcggtgaa gtattcaaga
      781 aactgactgc gtaaggcggc gcattccttg agcgcctcgc tcatcgctgg atagttgtat
      841 atccaatcag agccattcac agattcggtc ttgcgaggca tgacattgag atagaggttg
      901 tccaggaagg ccatcttcac cacatcaggg gctgccgtga cgatgcaact gattcgggga
      961 ctggggaaga cgcttgtgaa ggcctgacag tttcgaaatc ccccccagtt ccaggtg
//
LOCUS       DAOQUC010001676         1017 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_223877_length_1017_cov_2.495842, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001676 DAOQUC010000000
VERSION     DAOQUC010001676.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1017
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_223877_length_1017_cov_2.495842"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            5..>1017
                     /gene="fliG"
                     /locus_tag="PKH07_20820"
     CDS             5..>1017
                     /gene="fliG"
                     /locus_tag="PKH07_20820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011971744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HNT37450.1"
                     /translation="MKERSGSREKSLSGRRKAAILLVELGPEKSAEVYKNLSDEEIEE
                     ITIEIATLGTVNKDSSSVVVEEFYHTALAKQYINQGGIDLAKDILERAVGSKRAEEIV
                     GRLQGALQVSPFDFLKKVDPAHLLSFIQSEHPQTIALILAHLDYDPASKILGALPSET
                     QAEVASRLATMDQTTPEIINEVERILERKIASVYSQEFSAAGGVEALAELLNRVDRST
                     EKLILETLEERNQELADEIKKLMFVFEDVIQLDDKSVQQVLKEIDQKDLATALKGASE
                     QVREKIFHNMSQRAAESIKEDMEFMGPVRLRSVEEAQQRVVSAVRRLEEAGEIVISRG
                     GEE"
BASE COUNT          263 a          235 c          299 g          220 t
ORIGIN      
        1 gccgatgaag gaacgttccg gaagtcgtga aaagagtctt tccggccgac gcaaggccgc
       61 cattcttctg gtggaattgg ggcccgagaa gtctgcggaa gtatacaaga atctttccga
      121 tgaagagatt gaagagatca ccatagagat tgccactctt ggtactgtga acaaagattc
      181 cagttccgtt gtggtcgagg agttctacca caccgcactt gccaagcagt acatcaacca
      241 gggcggcatt gatttagcca aggatattct ggagcgtgca gtcggatcca agagagcgga
      301 agagatcgtg ggacggcttc agggagcctt gcaggtcagt ccctttgatt ttctgaagaa
      361 agtggatccg gcgcatctgc tgagttttat ccaaagtgag catcctcaga ccatcgcgtt
      421 gattctggcg caccttgact atgatccagc gtccaagatt cttggagcac tgccatcgga
      481 aactcaggcc gaagtggcat cacggttggc cacgatggat cagaccacac ccgaaatcat
      541 caatgaagtt gagcgaattc tggagcgcaa gatcgcctct gtctactctc aggagttctc
      601 tgcggcgggc ggagtcgaag ccttggcgga actgttgaac cgcgttgacc gaagcaccga
      661 aaaattgatt ctggaaacgc tggaagagcg aaatcaggag ttggcagacg agatcaagaa
      721 actgatgttt gtgtttgaag acgtcatcca actcgatgac aagagcgtac agcaggtact
      781 caaagagatc gatcagaagg atcttgccac ggcactcaaa ggcgcttccg agcaggttcg
      841 ggagaagatc ttccacaaca tgtctcaacg tgctgccgag agtatcaaag aggatatgga
      901 gttcatgggc cctgtcaggc ttcgaagtgt tgaggaagcc cagcagcggg tcgtttctgc
      961 ggtgcgtcgt ttggaggaag ccggtgagat tgtcatctca cgcggaggcg aagagga
//
LOCUS       DAOQUC010001677         1017 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_223942_length_1017_cov_2.145530, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001677 DAOQUC010000000
VERSION     DAOQUC010001677.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1017
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_223942_length_1017_cov_2.145530"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..277
                     /locus_tag="PKH07_20825"
     CDS             <1..277
                     /locus_tag="PKH07_20825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37451.1"
                     /translation="KGDAYAWCKGLVCKIGIEQEEDSCVTPDGTVWAKRSGLVQEIGV
                     LAREDTHHDLDGNAYRWCGDAVNYIRIQQEGDVNVTSDRRIWQRTNG"
     gene            347..>1017
                     /locus_tag="PKH07_20830"
     CDS             347..>1017
                     /locus_tag="PKH07_20830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37452.1"
                     /translation="MNKNNDVIYISPEAGRKLLKRMLFSLLTIFLVISLLWYGSNYWT
                     KLRTARQVAENTRKEVAGSLGYESYEKMVETLTAPCLNFWSGVAMQESKFGYVTSEKL
                     IPLRAIASRPNVYLFETYETISPMGVTGVVGTMNVWYPYFVFERRDNGILVGPSLRTP
                     ESERRWTPIGLCFCWTTRECLNLERDLPTYDSLEDARLEANGKPYMYRFADHFIKDSS
                     KKDVAT"
BASE COUNT          271 a          200 c          287 g          259 t
ORIGIN      
        1 gaaaggcgat gcatacgcat ggtgcaaggg tttggtctgc aagattggga ttgaacagga
       61 agaggattcc tgtgttactc ctgacggtac tgtctgggca aagcgttctg gcttggtgca
      121 ggaaatagga gtgcttgcaa gagaagatac tcatcatgat cttgacggca atgcttatcg
      181 atggtgtggc gatgctgtca attacattcg aattcagcaa gaaggtgacg taaatgtaac
      241 aagcgatcgc cggatatggc aacggaccaa tggatgagta gtggacacag gggtgtcctt
      301 ggcaaagtat gcccaattgc aagatttctc cggaggaatg tggggagtga acaagaataa
      361 cgatgtcatc tatatatcgc cagaggcggg aagaaaactc ctcaaaagga tgctattttc
      421 tctcctaact attttcctgg tcatttctct gttatggtat ggttcgaatt actggaccaa
      481 actgagaact gctaggcaag tggccgagaa cacgcgcaaa gaagtggctg gtagcctggg
      541 ttacgaatcg tatgagaaga tggttgaaac tctcacggct ccctgtctga atttttggag
      601 tggtgttgcc atgcaagaaa gtaaattcgg ctatgtgacc tcggagaaac tgataccgct
      661 gcgggccatt gccagtcgtc ctaatgttta tctctttgaa acatatgaga ccataagtcc
      721 catgggtgtt acgggcgtcg tgggcactat gaacgtctgg tatccgtatt tcgtttttga
      781 aagacgtgac aatgggatac ttgtcgggcc ttctctgcgc acacctgaaa gcgagcgacg
      841 atggacgccg ataggcttat gtttctgctg gacgacgaga gaatgcctta acctggagag
      901 ggatttgccg acatatgata gtttggaaga tgcacgactg gaggcgaacg gcaagccgta
      961 tatgtatagg ttcgccgatc acttcattaa agactcttcg aagaaagatg tagccac
//
LOCUS       DAOQUC010001678         1015 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_224600_length_1015_cov_2.465625, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001678 DAOQUC010000000
VERSION     DAOQUC010001678.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1015
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_224600_length_1015_cov_2.465625"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..636)
                     /locus_tag="PKH07_20835"
     CDS             complement(<1..636)
                     /locus_tag="PKH07_20835"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNT37453.1"
                     /translation="MITRTEIASLPVAAEVDVLVVGGGPAGVAASLSAARLGVRTMIV
                     EQFNCLGGIATAGGHAHICLYSEWGSPRRVVGGIPFETAQRVTSAGYGVHNNHEADFE
                     IEGMKKVLEEMAEQAGVTLRYYTLFAETIVEDTRVVGVVVQSKSGREVILARRTVDCT
                     GDGDVAASAGCKFLMGAEDSGPCQPATLMFTIGGVDYDRVRKFRGTDYKLKS"
BASE COUNT          233 a          290 c          243 g          249 t
ORIGIN      
        1 ggatttcaac ttgtagtcgg tgccacggaa tttcctgact cgatcatagt ccacaccacc
       61 gatggtgaac attagcgttg ccggctgaca gggacccgaa tcctcggctc ccatcagaaa
      121 cttgcagcct gcacttgcgg cgacatctcc gtcgccggtg cagtcaactg ttcttcgggc
      181 aagaatgact tccctgccac tcttgctttg aacaaccaca cccaccaccc tggtgtcctc
      241 aacaatcgtc tccgcgaaaa gcgtatagta acgaagtgtg acacccgcct gctctgccat
      301 ctcttcaaga accttcttca ttccctcgat ctcaaagtcc gcttcatggt tgttgtggac
      361 tccgtagccg gctgaggtca cccgctgtgc tgtctcgaaa ggaatgccac ccaccaccct
      421 tctgggagat ccccactcgg aatacaagca gatgtgagcg tggccacccg cagtggcaat
      481 gccacccagg cagttgaact gctcaacaat catcgtccga acccctagcc gcgcagcgga
      541 aagtgacgca gcaaccccag cggggccccc acctacaaca agcacatcca cctcagcggc
      601 aaccgggagt gaagcgattt ctgttcgagt aatcatccta tgtctcctta atttcaaaag
      661 gctttgaggc tcttggggca tgttctaatc tgtccggatt caagattttg gaccgaaaat
      721 ctcactctct cgatcatagt gtttgctgga tggaaagcaa gcgggaacag agtcatgagg
      781 gattcaggca gcagaaaatg ttcgtgccgg tatgaaagtt gccaaaaatc cttgcatttc
      841 tatacaggat tgatattttg cagagcatgt tggaaccatt ttctgttgtg atgatcgcgc
      901 ttatcctgac catgaccagt acagttggag ccatgatggg gtatggcatg gctgtgatcg
      961 cgcttccatt ctgcgccttc tttatggact tgaagatgct gccccctctg tttgg
//
LOCUS       DAOQUC010001679         1014 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_224903_length_1014_cov_2.986444, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001679 DAOQUC010000000
VERSION     DAOQUC010001679.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1014
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_224903_length_1014_cov_2.986444"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1014)
                     /locus_tag="PKH07_20840"
     CDS             complement(<1..>1014)
                     /locus_tag="PKH07_20840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37454.1"
                     /translation="KLNSIQLSFTGTWTQYDLEALALLDGGLALYRDDGNGVFGTGDL
                     AIPVVLASIPTWTGSNPYSVNLIPASPIDIPDIQDNLTDLYVVVRLSDTCVYGHTIGA
                     CINPQGLKFSTGDSLKTLCTKSLGIQVNTAPTITVLTPPAQGALADNSYLIQWTDDDP
                     DGNASISLYYDTTNSGLMGTLIASGIQEDLDGINDTYTWNTSSIPEGDYYIYAVINDG
                     HNTAHSYSKGVVRIRHSYVVTVNDLIQPNALGVQWIDADSQPTAVLGLNISDRGARAS
                     LQSVKLFVEDVSVFPGSFETTDLEQVRGETEYGVMVYRDDGTLEGVFDQTDTPLAVGP
                     ATWN"
BASE COUNT          245 a          276 c          259 g          234 t
ORIGIN      
        1 attccaagtc gctggaccca cggcaagagg cgtgtctgtt tggtcaaaca ctccctcaag
       61 agttccatcg tcacggtaga ccatcacacc gtattcggtc tcacctcgaa cttgctccaa
      121 gtccgttgtc tcaaaagaac cagggaacac cgaaacgtct tcgacaaaca gtttgacaga
      181 ctgcagtgac gcacgcgctc cccgatcgct gatattgaga cccagcactg ctgtcggttg
      241 cgaatcagca tcaatccact gcacgccaag cgcattgggt tgaatcagat cgttgactgt
      301 cactacatag gagtggcgaa tgcgcacaac gcctttggag taactgtggg cagtgttgtg
      361 accgtcgttg atgactgcat aaatgtagta gtcaccctcc ggaatgctcg aggtattcca
      421 tgtgtaggta tcattgattc cgtcgagatc ttcctgaatg ccactggcaa tcagcgtccc
      481 catcaacccg gaattggttg tgtcataata gaggctgatg ctggcattgc cgtcgggatc
      541 atcatccgtc cattggatga gataggaatt gtccgccagc gctccttgcg cgggcggagt
      601 caggacggta atcgtcggcg cagtgttcac ctgaatgccg aggctctttg tgcagagggt
      661 cttcagagaa tcccccgtac tgaacttcag tccttgagga ttgatgcacg ctccgatcgt
      721 atgcccgtag acacaggtat cactcaagcg caccaccaca taaagatcag tgaggttgtc
      781 ctgaatatcc ggaatatcaa tcggcgatgc cggaatcaga ttcacgctgt atgggttgct
      841 gcccgtccag gtgggaatgg aagccaagac caccggtatc gccagatcgc cggttccaaa
      901 gacaccattc ccatcatccc gatagagtgc caaaccaccg tccagaagcg ccaacgcctc
      961 aagatcatac tgcgtccagg ttcctgtaaa ggagagttga atcgagttta gttt
//
LOCUS       DAOQUC010001680         1014 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_224927_length_1014_cov_2.774765, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001680 DAOQUC010000000
VERSION     DAOQUC010001680.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1014
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_224927_length_1014_cov_2.774765"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..500
                     /locus_tag="PKH07_20845"
     CDS             <1..500
                     /locus_tag="PKH07_20845"
                     /inference="COORDINATES: protein motif:HMM:NF013978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bis-aminopropyl spermidine synthase family
                     protein"
                     /protein_id="HNT37455.1"
                     /translation="RTPLPRNLGSFDTFATDPPYTVAGCRLFVSRALERMTKDGTGMG
                     FLCFGQSRSDLLAEVQKSLCGMGLVVQEMIPMFNRYLGCSVLANSSALFHLSVSTDAA
                     PLIRDVYEGSLYTRDSKGKAKKYHCMKCKGEYEVGPGHPIQTIEALKEHGCSSCGGRK
                     FKAYG"
     gene            493..867
                     /locus_tag="PKH07_20850"
     CDS             493..867
                     /locus_tag="PKH07_20850"
                     /inference="COORDINATES: protein motif:HMM:NF014705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine decarboxylase"
                     /protein_id="HNT37456.1"
                     /translation="MAEPSSRKHVLVDWWIRDVQWLEHSGPMRTELISATQLFGFNVV
                     HDHFHDFDPVGYTGFLLLSESHLSVHSWTDEGYLALDIFSCRTGNLSALVEYLRNRLS
                     PYRECFLERPRGVIERLGGRNR"
BASE COUNT          238 a          237 c          265 g          274 t
ORIGIN      
        1 tgcgtacgcc attgcctaga aatctgggga gtttcgacac ctttgcgact gatcctcctt
       61 acacagtcgc aggctgccga cttttcgtga gtcgtgcctt agaacggatg accaaagacg
      121 gaacggggat gggatttctc tgctttggac agtctcgttc cgatctactt gcagaagttc
      181 aaaaatcact ctgtggcatg gggctcgttg ttcaggagat gattcccatg ttcaacagat
      241 atctgggatg ctcggtgctg gccaactcaa gtgccttgtt tcatctttct gtttcgactg
      301 atgctgcccc cttgattcga gatgtctacg aaggaagtct ctatacgagg gactccaagg
      361 gtaaggccaa gaagtatcac tgcatgaaat gcaaaggcga atacgaagtc ggtccgggac
      421 acccgattca gacaattgag gcactcaaag agcatggatg ttcctcttgc ggtggaagaa
      481 agttcaaggc ttatggctga gccctccagc cgaaagcatg tcttggttga ctggtggatt
      541 cgggatgtcc aatggcttga gcattctgga cccatgcgaa cagagttgat ttcagcaaca
      601 caactgtttg ggttcaatgt tgtccatgat catttccacg atttcgaccc cgttggttac
      661 acgggttttc tgcttctgag cgaatcgcat ctttccgttc acagttggac tgacgaagga
      721 tatctggctc ttgacatatt ctcctgtcgg acgggaaacc tatcggcact ggttgagtat
      781 ctaaggaaca gactgtcacc ttaccgcgag tgttttcttg aaagacccag aggggtgatc
      841 gagagattgg gaggaaggaa ccgatgaagt tacgagcaat tattgttgtt tcagccgcta
      901 ttgttcttct cgtgttgggg catctcttcg tcttcgcccc actggcatca caatggtcgc
      961 aatccacaga caagttcagc aataatgttc aacaggtgca acgaatcatc cggg
//
LOCUS       DAOQUC010001681         1013 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_225224_length_1013_cov_3.540710, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001681 DAOQUC010000000
VERSION     DAOQUC010001681.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1013
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_225224_length_1013_cov_3.540710"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..554)
                     /locus_tag="PKH07_20855"
     CDS             complement(<1..554)
                     /locus_tag="PKH07_20855"
                     /inference="COORDINATES: protein motif:HMM:NF040466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdjY domain-containing protein"
                     /protein_id="HNT37457.1"
                     /translation="MLTRNDPVDRCCTVHGGRSWVWLFSLVFVAFMAVNADAQLPQNV
                     QKAEPPRDRLQEALQSADVKPLGEGKFQIGAVVIDQQEKCVRVPGRVNMTQGPIELLA
                     CAPGGRTHESVLVLDVEPIHVQTGLLLIGLQPGQSVRIEGDPVVPKGSAVVVDVTWEL
                     QGVKSEYRGEDLVLEVKSKETMPRT"
     gene            complement(652..>1013)
                     /locus_tag="PKH07_20860"
     CDS             complement(652..>1013)
                     /locus_tag="PKH07_20860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37458.1"
                     /translation="TKRRWKTGVRKILALVGVLLFVYSSGIGRATAKNSAFAVFEFQS
                     VSGLQSRLYLVYLASRAPFEIHVPPGQIPWFPDPVEASRVLEQVRVSPDNGMVTLVPR
                     IAGARAMFICQPVGTGT"
BASE COUNT          245 a          300 c          250 g          218 t
ORIGIN      
        1 gttcgaggca tggtctcctt ggacttgact tccagaacaa gatcttcacc gcggtactcg
       61 gacttgactc cctggagttc ccaagtcaca tccaccacaa cggcacttcc cttgggtaca
      121 accggatcgc cttcgatccg aacgctttga ccgggctgca gaccgatcag aagcagtccg
      181 gtttgcacat ggatcggctc gacgtccaac accagcacag attcgtgagt tcttccacca
      241 ggagcgcagg caagcagttc tatgggacct tgggtcatgt tgacgcgccc tggaacgcga
      301 acgcacttct cctgctgatc gatgacaacg gctccaatct gaaacttgcc ttctcccaac
      361 ggcttcacgt cggcggactg cagagcttcc tgaagtcggt cgcggggagg ctctgccttc
      421 tgaacattct gtggcagctg cgcatcagca ttcacagcca taaacgccac aaacacaagg
      481 gaaaacagcc acacccaaga gcgaccacca tgaacggtac aacacctgtc aacagggtca
      541 tttcttgtga gcattgtctg cacctctcat cagagtccgc tctctatcag cggcaaatga
      601 ttttcaactt atactcttct ggccattggg gatcaagcgg gcaccaaggg ttcatgtccc
      661 tgtgccgacc ggttggcaga tgaacatcgc tcgagcgcct gcaatgcgtg gcaccaaggt
      721 gaccattccg ttgtctggag ataccctcac ctgctcgagc actcgagatg cttccactgg
      781 gtcgggaaac cacggtatct gtcctggtgg aacatggatc tcgaaaggcg ctcttgaagc
      841 aagatatacc agatacaagc gggactgtag tccggaaaca gattggaatt caaacacagc
      901 gaaagccgag tttttggcgg ttgctctgcc aatcccagag gaataaacaa acaacaagac
      961 acccaccaag gccagaattt ttcgtacacc ggtcttccat cgcctcttgg tcg
//
LOCUS       DAOQUC010001682         1013 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_225360_length_1013_cov_2.374739, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001682 DAOQUC010000000
VERSION     DAOQUC010001682.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1013
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_225360_length_1013_cov_2.374739"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1001)
                     /locus_tag="PKH07_20865"
     CDS             complement(<1..1001)
                     /locus_tag="PKH07_20865"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNT37459.1"
                     /translation="MLRRIEVTGTLESESFVEIMPEIPGTVVERRAEEGDRAQAGQIL
                     AVLDKTEIEIEVKRAQANLQQRQAELSQLLAGNRTEDIAVARAARDRAKAGHDNMLRN
                     RERYQALHEKKIFTDKDWDDFIAQLDQARENLEAAEAELSKAQTGPRREEIEKARASV
                     AAASADLALSRKRLSDTEIKAPFNGIISKRYLEVGERVETNRPVFSIYDDTALRVSVT
                     VPENYIGVLAQGQRCEITVPAYPGEIFEGTVRVVGEQLDVTTRSLPVKVIVKSKEHRL
                     LPGMFARVTVEMGWIDQAVVIPRDAVRFREGMPIAFVVKDGRAYERALDLGQSSMDQ"
BASE COUNT          199 a          315 c          244 g          255 t
ORIGIN      
        1 atttgatcca tgcttgactg tcccaagtcc agggcacgtt cataggcacg accgtccttg
       61 acaacaaaag caataggcat cccttcgcgg aaacgtacag catctcgtgg tattaccacg
      121 gcctgatcga tccaacccat ttcaacggtc acccgcgcga acattccggg aaggagcctg
      181 tgctctttgg atttcacaat aaccttcact ggcaaactgc gtgtggtgac atccagttgc
      241 tcgccgacga ctcggacagt gccttcaaag atttctccag gataagcagg tacagtgatc
      301 tcgcagcgct gcccctgagc cagaacacca atgtagttct ctggtactgt aacactgact
      361 cgcagtgcag tgtcgtcgta gatcgagaaa accggtcggt tggtctccac tctttctcca
      421 acttcaagat acctcttgga gatgataccg ttgaaaggag ccttgatctc ggtgtcactc
      481 aggcgtttcc ggctgagcgc cagatcggca ctggctgcgg ccacagaggc gcgtgccttc
      541 tctatttcct cccgccgtgg accagtctgt gccttgctca actccgcttc ggcagcttcc
      601 agattctccc tcgcctgatc caactgagca atgaaatcat cccagtcctt gtcggtgaat
      661 atcttcttct cgtgcagagc ctgatatcgc tcgcggttac gaagcatgtt gtcgtgtcca
      721 gcctttgccc tgtctctggc tgctcgggca acagcaatgt cttccgttcg attgcccgcc
      781 agcagttgag acagttctgc ctgacgctgc tgaagattcg cctgggcgcg tttgacttct
      841 atctcgatct cggtcttgtc cagcacggcg agaatctgcc ctgcttgtgc ccggtcgcct
      901 tcctccgccc tccgttcaac gactgttcca gggatttccg gcatgatctc gacaaacgac
      961 tccgactcaa gcgttcctgt gacttcaatg cgccgaagca tgcgatcccc ctc
//
LOCUS       DAOQUC010001683         1013 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_225411_length_1013_cov_2.151357, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001683 DAOQUC010000000
VERSION     DAOQUC010001683.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1013
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_225411_length_1013_cov_2.151357"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1013)
                     /locus_tag="PKH07_20870"
     CDS             complement(<1..>1013)
                     /locus_tag="PKH07_20870"
                     /inference="COORDINATES: protein motif:HMM:NF019908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NPCBM/NEW2 domain-containing protein"
                     /protein_id="HNT37460.1"
                     /translation="SLLRFHLSKGTVTVSLQDVLNNGAVYVPDYGLFISSLPEKQTLS
                     DYLKSIESRKTVLEQVCELPEQTRQQAMAKTHYNPALDTGRMMLSLACGNDKFITQRD
                     GTITFVEDYTRDERGRQPTLHLASVFGRQSLLGFTQGWGNPGINKAAHSLPDLALPMQ
                     IKDKHYSAGLGHHANGEILIGLQGRYSRFQAEVGLQWQGGNTPGSVVFEVLLDEKTVF
                     NSGIMREHDDPKSIDLDVTGVQRLMLRVGDAGDGIGFDAANWAEARLTSADPSDADEF
                     LTEFFEKVPEYTRHLSGGWLPAPVVTTVMGDLFYSQRTFVVPYGADLEQTSPEWFSPQ
                     ALC"
BASE COUNT          240 a          285 c          254 g          234 t
ORIGIN      
        1 acagagcgcc tgaggactga accattcagg agacgtctgc tccagatctg caccataggg
       61 caccacgaat gtcctctgac tgtaaaacag atcgcccatc accgttgtca cgactggtgc
      121 tggaagccat cccccactga gatgacgcgt gtattccgga actttctcga agaactctgt
      181 caggaactca tcggcatccg aggggtctgc agacgtcaga cgtgcctctg cccagttggc
      241 agcatcaaag ccgataccgt caccagcatc tccaacacgt aacatgagac gctgcacacc
      301 cgtcacatca agatctatgg acttgggatc atcatgctct ctcatgatcc ctgagttgaa
      361 aaccgtcttc tcgtccagca gcacttcaaa gacaacactt cctggagtat tgccaccctg
      421 ccattgaagc ccgacttctg cctgaaatcg ggagtacctg ccttgaaggc caatcaggat
      481 ttcaccgttg gcatgatggc caagacccgc cgaatagtgc ttgtccttga tctgcatcgg
      541 caatgccaga tcgggtagtg agtgggcagc cttattgatt cccgggttgc cccatccctg
      601 agtaaatccc agcaggctct gtctgccaaa aacagacgca aggtgcaacg tcggctgccg
      661 tccccgttca tctcgagtgt aatcttcgac gaaggtaatg gttccatccc gttgtgtgat
      721 gaacttgtca ttgccacacg caagagagag catcattcga cccgtgtcca acgccgggtt
      781 gtagtgtgtc ttcgccattg cctgctggcg ggtttgctca gggagttcgc aaacttgttc
      841 gagcacggtt ttccgtgact cgatcgattt gagataatca gagagcgtct gcttttctgg
      901 cagactgctg ataaacagac catagtctgg gacgtagaca gcaccgttgt tgagaacatc
      961 ctgaagcgac acagtaacgg tacctttgga cagatggaac cgaagcagcg att
//
LOCUS       DAOQUC010001684         1013 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_225524_length_1013_cov_1.696242, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001684 DAOQUC010000000
VERSION     DAOQUC010001684.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1013
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_225524_length_1013_cov_1.696242"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(154..>1013)
                     /locus_tag="PKH07_20875"
     CDS             complement(154..>1013)
                     /locus_tag="PKH07_20875"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNT37461.1"
                     /translation="ADDEPTDRILSFRRTWHQQHENPPADVEPRDYVSLRRRNIRGYY
                     GNITHADWAIGQILSCLEQEGIVEDTIIVYTTDHGDIACEHGILEKAPGINSDSVCRI
                     PYIWSWPGHLPQGVERSQLVQAIDMWPTLTSLAGLSEMSMWDGRNITDCLMDERKKVH
                     EAVFTENPFLKGITTERWRMIRVPEGMFPGDPIRGELYDRCKDPWERTNLFHSPTYSS
                     IVQELTEKMLDWLITSHRPISALPVLPHPSVTWADDPMNDPKPHPEDGKTSPNQIWDC
                     IRKGIDNYL"
BASE COUNT          217 a          260 c          280 g          256 t
ORIGIN      
        1 atgacgggtt gcacaaagat gatatagcac agcgtgacaa atgtggtcgt gcccgcgatg
       61 atctcagtgc gaacacaggt tcgattttct ttcaggtgga agagtctctc aagcattcct
      121 ggttcctccc tgtccaagcc gccgaagagg atcctacagg tagttgtcaa ttcccttgcg
      181 gatgcaatcc caaatctggt ttggcgatgt cttgccatct tcaggatgcg gcttgggatc
      241 attcattgga tcatcagccc aggtgacgga cgggtgtggc aacaccggca acgcactgat
      301 cggacgatga gacgtaatga gccagtcgag cattttctcg gtcagttcct gaacaatgct
      361 ggaataggtg ggactatgaa acagattggt gcgctcccag ggatctttgc atcggtcata
      421 gagttctcct cggatgggat ctcctggaaa catgccttcg ggcactcgga tcatccgcca
      481 gcgctcggtt gtgatgccct tgaggaaggg gttttcggta aagacggctt cgtgtacttt
      541 cttgcgttca tccatcaggc agtcggtgat gtttctgccg tcccacatgc tcatctcgga
      601 aagcccggcc agactcgtca gcgtcggcca catgtcaatg gcctgaacca gttggctgcg
      661 ctcgactccc tgtggaagat gcccaggcca tgaccatata tagggaattc tgcagacgga
      721 atccgagttg atgccaggtg ccttttccaa gattccatgt tcacaggcga tgtcgccatg
      781 atccgtggtg taaacaatga tggtatcttc gacaattcct tcctgttcga gacaagaaag
      841 aatctgacca attgcccagt cggcatgtgt gatgttcccg tagtagccgc ggatattgcg
      901 acgtctcaag gatacgtagt cccgcggctc cacatctgcc ggtggattct catgctgttg
      961 gtgccaggta cgtcggaagg aaagaatcct gtccgttggt tcatcgtccg cgc
//
LOCUS       DAOQUC010001685         1012 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_225639_length_1012_cov_3.533960, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001685 DAOQUC010000000
VERSION     DAOQUC010001685.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1012
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_225639_length_1012_cov_3.533960"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..211)
                     /locus_tag="PKH07_20880"
     CDS             complement(<1..211)
                     /locus_tag="PKH07_20880"
                     /inference="COORDINATES: protein motif:HMM:NF019845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HNT37462.1"
                     /translation="MKAAVYVSKDTIEMQDLPIPKPGVGEILLKVTACGICGTDCHIL
                     AGEVPLAKPPVVLGHEICGTVAELGK"
     gene            complement(304..>1012)
                     /locus_tag="PKH07_20885"
     CDS             complement(304..>1012)
                     /locus_tag="PKH07_20885"
                     /inference="COORDINATES: protein motif:HMM:NF024584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HNT37463.1"
                     /translation="VLTNIRQFMQLRAERGSPLPYPRLSFVMLRDNLHEIPGCADFAK
                     SLGVEFIAVMDPVFYSQETAERFSFARGEGEEAIEELRKRCVQLGVFLDYYEDSPSIY
                     FDSKGMPTCPGKTPPALRYQDDPSPLFCPMLYTTLFIRGDGKVAPCCYLYEKVVGDLT
                     VSSITEIWRGDKLAQMRSAIRERNFPPGCRICQRLLLLRRREVMTKGRVFVHRTWQAS
                     RERRQTGYAAQVQASII"
BASE COUNT          242 a          293 c          253 g          224 t
ORIGIN      
        1 cttttccaag ttcggcgacc gtgccacaaa tctcgtgccc gaggacaaca ggaggctttg
       61 ccaatggaac ctcccccgcc aggatatggc agtccgttcc gcaaatgcca caggcggtca
      121 ccttgagcaa tatctccccc accccaggtt tggggatcgg caagtcctgc atttcaatgg
      181 tatctttact gacgtaaact gctgctttca tgatcatacc tcccggactg gtctcagtat
      241 tccagcccaa gcctaagcgt atcacaatcg tcttggcttg ctcaacaggg aacagtggga
      301 ggactagatg atcgaagctt gtacttgagc agcgtatcct gtctggcgtc tttcacggga
      361 agcctgccat gttcgatgaa cgaaaacccg tcctttcgtc atcacctccc gtcgcctcag
      421 gagcagaaga cgctgacaga ttcgacatcc cggggggaag ttgcgttccc gaatggcgct
      481 ccgcatttga gccagtttgt cgccacgcca tatttcggtg atgctggaga cagtcagatc
      541 tccaacaacc ttctcataga ggtaacagca tggcgccacc tttccatcgc ctcggatgaa
      601 aagagttgta tagagcatag ggcagaaaag tggtgaagga tcgtcctggt atcgaagcgc
      661 gggaggcgtc tttcctgggc aggtcggcat ccccttggag tcaaaataga tggagggact
      721 atcctcatag taatcaagaa acacacccag ctgaacacag cgcttccgca gctcttcgat
      781 cgcctcttcc ccttctccac gggcgaacga aaaccgttcg gccgtctctt gcgagtagaa
      841 gacaggatcc atcactgcaa taaactccac acccagcgac ttggcaaagt ctgcacaccc
      901 cggaatctca tggagattgt cacgtaacat cacaaaactc agccgaggat acggcagtgg
      961 gctgcctcgt tccgcacgca gttgcatgaa ctgtcggatg tttgtcagaa cc
//
LOCUS       DAOQUC010001686         1010 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_226647_length_1010_cov_2.047120, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001686 DAOQUC010000000
VERSION     DAOQUC010001686.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1010
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_226647_length_1010_cov_2.047120"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..319)
                     /locus_tag="PKH07_20890"
     CDS             complement(<1..319)
                     /locus_tag="PKH07_20890"
                     /inference="COORDINATES: protein motif:HMM:NF015728.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RAMP superfamily CRISPR-associated protein"
                     /protein_id="HNT37464.1"
                     /translation="MAETSCKECLHLFRAELVLDTALHIGGDEEEYLFGPAPCLMDEG
                     TPYIPGSSIAGALIDLILSSGRAQGDIKALSCKNQKPEDFIASAVSVRSAYPIASKEI
                     RFQV"
     gene            complement(312..>1010)
                     /locus_tag="PKH07_20895"
     CDS             complement(312..>1010)
                     /locus_tag="PKH07_20895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37465.1"
                     /translation="KDISLVDKGWQESGVLRGFSPMGGLPRMPARAVLRGSCFAVQID
                     DQSKKEEVRKVLFDAWRKGLGERTVEGLGRFHIVIPGDSTSQTSKTVTDGGTDKKNQT
                     ESVPETIADLKEQVLQELDGLFEKELKGLVKNGPSKSQWASFAALLLSTKDDDSVNEA
                     IEVLNKRKDKKSGKKWAEKVAGKELPMWMKGKFAEWNRHCQLGRHFLLMLARRAVQIQ
                     KNDSTQDRTEGKNG"
BASE COUNT          233 a          296 c          201 g          280 t
ORIGIN      
        1 tgacctggaa acgtatttcc ttggaagcaa tcggataggc agagcgcact gaaaccgccg
       61 atgcaatgaa atcctccggc ttctgattct tgcatgacaa ggctttgata tccccttgcg
      121 ctctaccaga actcaatatc agatcaatca gagcgcctgc gatactgctt ccgggaatat
      181 acggagttcc ctcgtccatg agacagggtg ccggaccaaa aaggtattcc tcttcatcac
      241 caccaatgtg cagtgcggtg tccaatacca actccgcgcg aaaaaggtgc agacattcct
      301 tgcaactcgt ttcagccatt tttcccctcc gttctgtctt gggtagagtc attcttctga
      361 atctggacag cgcgcctcgc cagcatcaga agaaaatgac gccccaattg gcaatgccta
      421 ttccactcag cgaacttacc cttcatccac ataggaagct ccttgcctgc aaccttctca
      481 gcccatttct tcccgctctt cttgtccttt cttttgttga gaacttcaat tgcttcgttg
      541 actgaatcat catccttcgt agacaacaag agcgctgcaa acgaagccca ctgtgatttc
      601 gaaggtccat tcttgacaag tcctttcagt tctttttcga acagaccatc aagttcctga
      661 agcacttgct ctttcagatc tgcgattgtc tccggaacac tttctgtttg attcttctta
      721 tcggttccac catcggtaac ggtttttgaa gtttgcgatg tcgagtcgcc tggtatgaca
      781 atatgaaatc ttcccagacc ttccactgtt ctctcgccaa gacccttgcg ccaggcatcg
      841 aacaacacct ttcgaacctc ttctttcttg ctttgatcgt ctatctggac agcaaaacag
      901 gagcctcgaa gcaccgctct cgctggcatc ctcggcagac cacccatggg tgagaaacct
      961 cgcaagacgc cggattcctg ccatcctttg tctacgagtg atatatcctt
//
LOCUS       DAOQUC010001687         1007 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_227679_length_1007_cov_2.765756, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001687 DAOQUC010000000
VERSION     DAOQUC010001687.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1007
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_227679_length_1007_cov_2.765756"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..968)
                     /locus_tag="PKH07_20900"
     CDS             complement(<1..968)
                     /locus_tag="PKH07_20900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37466.1"
                     /translation="MKRAVFILILPLTLFSVRVFAVPNEIESFDGTGAYSGFDLPSWS
                     TVGASGSFSVPAGQYKISSSGAPVDVGLERFVGQGDFESTLYVSNFTGWDTSMWQDGL
                     RWQFWDSYMQDGISVVLDSARYYPNKALWVDKYVSGVKTTTTQLNLGSTITSLKWRVL
                     WTETDKKWEVFYRINGGPAEPADVPAPDMPLTGYVLPSQSNRLTKILAYENISSTGVT
                     AWLDNYIMAPRFGETPTPTNTVTPTFTVNFDTPTPTPVGLSAPEVYVSQSYGNDVTGD
                     GTIDNPWASIVYALSQVNADSNRHVNIRVSQGVYIGQVQMKSWTNL"
BASE COUNT          254 a          270 c          253 g          230 t
ORIGIN      
        1 atcagattcg tccacgactt catctgaacc tgaccgatgt agactccttg agacacacgg
       61 atgttgacgt gcctgttact gtccgcattg acctgactca gtgcgtagac aatggacgcc
      121 cagggattgt ctatcgtccc gtcaccagtg acgtcatttc cataggactg ggatacatac
      181 acttccggag cagacagacc cacaggcgtt ggggtcggcg tgtcaaagtt cacggtgaac
      241 gttggggtga ccgtgtttgt cggtgtcgga gtctccccaa atctcggcgc catgatgtaa
      301 ttgtccagcc aggcagtcac ccccgtcgag gagatgttct cgtaagcgag gattttcgtc
      361 agtcggttgc tctgagaagg gagtacataa cccgttaagg gcatatccgg tgcagggaca
      421 tccgctggtt ctgctggccc accgttgatc cggtagaaga cttcccattt cttgtcggtc
      481 tctgtccaga gaacccgcca tttcagagag gtgatggttg agccaagatt caactgtgtg
      541 gttgttgtct tgactcccga tacgtatttg tccacccaaa gggctttgtt gggatagtat
      601 cgagcagagt ccaaaacgac cgagatgcca tcctgcatgt aggaatccca gaactgccat
      661 cgaagaccat cctgccacat tgatgtatcc caaccggtga agttgctgac atagagggtg
      721 gactcaaaat ccccctgccc aacaaatcgc tcaagaccaa cgtctaccgg agcgccgctt
      781 gaactgatct tgtactgacc tgcggggaca gagaacgagc cagaagcacc aaccgtagac
      841 caacttggta gatcaaagcc actgtaggca cccgtcccat caaacgactc gatttcgttt
      901 ggcacggcaa aaacacggac agaaaacagc gttagaggaa gaatcagtat aaagactgcc
      961 cttttcatga tcaaactccc ttcaaaagaa ataacactcc tctgatc
//
LOCUS       DAOQUC010001688         1007 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_227783_length_1007_cov_2.283613, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001688 DAOQUC010000000
VERSION     DAOQUC010001688.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1007
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_227783_length_1007_cov_2.283613"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            15..>1007
                     /locus_tag="PKH07_20905"
     CDS             15..>1007
                     /locus_tag="PKH07_20905"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNT37467.1"
                     /translation="MFGLQNQEQMVGRRVADFLVPRHKDYVDDLNVRRQKGEDVPSQY
                     QVLCTHADGSVFEVEVSASTFRYKERLASIALLRDISVRLKAEQALRESEERFRTFVE
                     HAAEGICVIQDAVIKYLNPHACELIDCLPEEAIGMPFDHFVEPADKQKVGLSYQKHLS
                     GDESYQVLQMNVLRKDGQRVEVEISLGPVTFDGRPGTLIFVRDLTERKRLEETIRQAQ
                     KTQALAVLAAGISHNFNNLLYIIRGNTEMLAHELDPANKKASEYLSEILTAVERASDM
                     VSQIVTFGRQTEQQFMPFLIQPILKESLRLIRNSKPPGVVLHQDIAPNCPPILGD"
BASE COUNT          242 a          250 c          288 g          227 t
ORIGIN      
        1 ctgcaatgct aagaatgttc ggattgcaga accaggagca gatggtcgga cgccgtgtgg
       61 ctgactttct cgtcccaaga cacaaagact atgtggatga tctgaacgtt cgaaggcaaa
      121 agggcgagga tgttcccagc cagtatcagg tactctgtac gcacgcagac ggttctgtgt
      181 ttgaagtgga agtgtccgcc agcacgtttc gctacaagga acgactggcc tcgatagcct
      241 tgcttcgaga catctcggtg cggctgaagg cagaacaggc cttgcgggaa agcgaagaac
      301 gctttcgaac tttcgtggag catgcggcgg agggaatctg tgtcattcag gatgcggtga
      361 tcaagtatct caatccacat gcttgtgaac tcattgactg cctgcccgaa gaggctatcg
      421 ggatgccgtt cgatcacttt gttgaacccg ctgacaagca gaaggttgga ctgtcctatc
      481 agaagcatct ttcgggcgac gagtcgtatc aggttctgca gatgaatgtg ctgcgcaagg
      541 atggccaacg agtggaagtg gagatcagtc tgggtcccgt cacgtttgac ggcaggcccg
      601 gaacgctgat tttcgtacgc gacttgacgg aacgaaaacg ccttgaggag acgatacggc
      661 aggcacagaa aacgcaggcg cttgccgtct tggctgcagg gatttcccac aacttcaaca
      721 acctgcttta catcatacgg ggcaacacgg agatgctcgc acacgagttg gatcctgcca
      781 acaagaaggc ttcggagtat ctcagcgaga tcttgactgc cgtggaaagg gcatctgaca
      841 tggtgtcaca gattgtcacg ttcggtcggc agacagaaca gcagttcatg ccattcttga
      901 tccagcccat tcttaaagag tcgcttcggt tgatcaggaa ttccaagccg cctggagtcg
      961 ttcttcatca ggatattgcc ccgaattgtc ccccaattct cggtgac
//
LOCUS       DAOQUC010001689         1006 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_228120_length_1006_cov_2.691903, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001689 DAOQUC010000000
VERSION     DAOQUC010001689.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1006
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_228120_length_1006_cov_2.691903"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..592)
                     /locus_tag="PKH07_20910"
     CDS             complement(<1..592)
                     /locus_tag="PKH07_20910"
                     /inference="COORDINATES: protein motif:HMM:NF019908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NPCBM/NEW2 domain-containing protein"
                     /protein_id="HNT37468.1"
                     /translation="MSLVRQWTSAKIEGVSVPAEPLAGLVVLANHDPVQRNVRAGRPL
                     KIGQQEFTRGLYCHATSRIHVRLPQPARAFTALVGVDHNSDTSGGRGSVVFSVSVGGQ
                     EKFHSSVMRGGEPGVAVNVDLDGASDFLLDISDAGDGIACDQADWADAKVTLSDGTDL
                     WLGEMALIDEQEAPISTDPYFSFVYDGEPSATLLSHW"
     gene            complement(838..1002)
                     /locus_tag="PKH07_20915"
     CDS             complement(838..1002)
                     /locus_tag="PKH07_20915"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNT37469.1"
                     /translation="MAVGRGIGAEIRNGVGVASVGGILVSGVLTLFLLPVIYDLFTRR
                     QRAKQTAEPR"
BASE COUNT          253 a          299 c          237 g          217 t
ORIGIN      
        1 cccagtggct gagcagagtt gcggaaggtt ctccatcata gacaaacgaa aagtatggat
       61 cggtgctgat gggggcttcc tgctcatcaa tgagagccat ctcacccagc cagagatctg
      121 tcccgtcaga aagagtaacc tttgcgtccg cccaatctgc ctgatcacag gcaatgccat
      181 cgccagcgtc tgagatgtcc aggaggaagt cactcgcacc gtctagatcc acgttcaccg
      241 ccacgccagg ctctccgcct cgcatcaccg acgagtgaaa cttctcttgt ccgccgacgc
      301 ttaccgagaa aacgacactt cctcgaccgc ctgaagtatc agagttgtga tctactccaa
      361 caagagcggt aaatgccctc gccggctgcg gaaggcgcac atggatcctg ctggtcgcat
      421 ggcagtaaag tccacgagtg aactcttgtt ggccaatctt gagtggccga cccgctctga
      481 cgtttctctg aacaggatcg tgattggcaa gaacgacaag gcctgcaagt ggttctgcgg
      541 ggacggacac tccttcaatc ttggcacttg tccactgacg aacgaggctc atctcctcct
      601 cagtcggctt caaagaaggt aaggcagttg cattttttga catcaggctg actcccagta
      661 tgaatagaag agctttgaat cgcatcaagg ctatcctgaa tctcaagtct gcgcaatctt
      721 tttgccactt cattaagcac ctcccaaaac cacagacagc atctttggtt acaggtcgtc
      781 ccgcactgag actcctgaca agtatacagc gcagaagacc atttccagag attcacccta
      841 tcgcggttct gcagtctgct ttgcccgctg ccttcgagtg aacaagtcat agatgacagg
      901 cagcaagaac aaggtcagaa ctccagaaac gagaatgcca ccaacactgg caaccccaac
      961 accatttctg atctcagcac caatccctcg accgactgcc agtggc
//
LOCUS       DAOQUC010001690         1005 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_228565_length_1005_cov_2.477895, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001690 DAOQUC010000000
VERSION     DAOQUC010001690.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1005
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_228565_length_1005_cov_2.477895"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..501
                     /locus_tag="PKH07_20920"
     CDS             <1..501
                     /locus_tag="PKH07_20920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37470.1"
                     /translation="RRYRMMRNLVLTFVILGCLCGSGFAEEVKQLEGMSNGAKAALLY
                     LPNRLMDFVDIFRLNVGVGEGWGANLRATNFLTGGVSDYDTIRFGMRGREMPRYEEAV
                     DEECIGLFGLQIGKFDRDPYELAFTGHVAYAGLEAGLDLDEIVDFLLGLACVDYREDD
                     MGPRVW"
     gene            584..>1005
                     /locus_tag="PKH07_20925"
     CDS             584..>1005
                     /locus_tag="PKH07_20925"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNT37471.1"
                     /translation="MGISRQDLFLDYRMMELTTLNEIAKVVNSTLDLQEVLDEIVRLT
                     TARMRADACAVFLLENQTMVLRAARGLNMELVNAFSLALGEGLTGYAAKVGKPLTEKD
                     IQSAGGFQLSDELGETDYVSLLSVPLIAKGRIVGVINV"
BASE COUNT          202 a          222 c          317 g          264 t
ORIGIN      
        1 aggaggtaca ggatgatgcg aaacctggtg ttgacgttcg ttattctcgg gtgtctctgc
       61 ggcagtggat ttgctgagga agtcaagcaa cttgagggca tgagcaatgg agccaaggca
      121 gcactgctgt accttccgaa tcggctcatg gattttgttg atatcttccg gctgaacgtg
      181 ggtgtgggtg aaggatgggg tgcaaatctt cgtgccacca attttctcac gggaggtgtt
      241 tccgactatg acaccatccg ctttgggatg agggggcgcg agatgcctcg ctatgaggag
      301 gctgttgacg aagaatgtat cggcctgttc gggctgcaga ttgggaagtt tgaccgggat
      361 ccttacgagt tggcgttcac cggacatgtc gcctacgccg gcttggaggc aggattggat
      421 ttggacgaga ttgttgactt cttgcttggc ttggcctgtg tggactatcg ggaagacgat
      481 atgggtccaa gagtctggta atccgcgttg actcttcgct acatccctgg gaacttacac
      541 tcaggaagag gcggtttctt ggagaagaca gggcgctttc tctatgggca tttcacgaca
      601 agacttgttt ttggattacc gaatgatgga gctgacgact ctcaacgaga tcgccaaggt
      661 cgtcaattcc actctggatc tgcaggaagt tcttgatgag atcgttcggc tgaccactgc
      721 ccgtatgcgg gcggacgcat gtgctgtctt tctcctggag aaccagacga tggttcttcg
      781 ggcggcgcgt gggctgaaca tggaactggt gaatgccttt agtcttgctt tgggcgaagg
      841 tctgactggc tatgctgcaa aggttggcaa gcccttgaca gagaaagata tccagtcggc
      901 gggaggcttc cagctttcag atgaattggg tgagaccgat tacgtctccc tcttgtccgt
      961 gccgcttatt gccaaggggc gcatcgtcgg cgtgatcaat gtcta
//
LOCUS       DAOQUC010001691         1004 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_228914_length_1004_cov_2.782929, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001691 DAOQUC010000000
VERSION     DAOQUC010001691.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1004
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_228914_length_1004_cov_2.782929"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="PKH07_20930"
     CDS             complement(<1..330)
                     /locus_tag="PKH07_20930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37472.1"
                     /translation="MFSQATTQYRGFDGISVVHLSILLSLSFLFTYPCCIQAAVVFNG
                     LEISEQGFREIPLESDTLQQRPSDAVQDTFQRPGTGTSVGSVSSSSLKERRFVVESGL
                     SAQELMAT"
     gene            555..>1004
                     /locus_tag="PKH07_20935"
     CDS             555..>1004
                     /locus_tag="PKH07_20935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37473.1"
                     /translation="MRVFVTVVLLCLCLVVGAQAEIKTLTFQQGLNGYDGVQDTFLYA
                     PDSVANVNYGNHPTVATGINRWGENLVTLIRFDLSDLPAEAKVLRASLFLFDTSKEWP
                     NDQISVSACLVAPANAGWIAGHNEGVRIPQQGTSCWNWMAYNTRPWAG"
BASE COUNT          243 a          254 c          261 g          246 t
ORIGIN      
        1 tgtcgccatc agttcctggg cgctcaggcc gctttccaca acgaaccggc gttctttgag
       61 gcttgatgat gaaacacttc caaccgacgt tcccgttcca ggtctctgga acgtatcctg
      121 cacggcatca cttggccgtt gctgaagcgt gtcggattcg agtggtatct cacggaatcc
      181 ctgctcagag atctccagcc cattgaaaac aaccgccgcc tggatacagc aaggatacgt
      241 gaagaggaat gacaacgaga gcagaatcga aagatgaacg actgaaatcc cgtcgaaacc
      301 tctatactgt gttgtagctt gtgagaacat tctactatcc tctcgggtgc acatttcctg
      361 agatcgtacc gcgatttcat ctctgtaaca acgtcagaat gatctggggt caaagagtgc
      421 aaagcccaat aatcgaaacg ttggtacacg cccatctcct gaacatgcct ttgacgatgc
      481 ggatctacag gagtatgttt tgatagttgt gcagctgata gcccggtcgt aacccaacaa
      541 ttggagagcg gaaaatgcga gtcttcgtca ctgtagtctt attgtgcctt tgtttggttg
      601 tcggtgctca agccgagatc aagacgctga cctttcagca gggactgaat ggctatgacg
      661 gtgtgcagga tacctttctc tacgctcctg actctgtcgc caatgtgaac tatgggaacc
      721 atccgaccgt cgcgacgggc ataaatcgtt ggggagaaaa tcttgtcacg cttatccgat
      781 tcgatctttc cgatctccca gcggaggcga aagtgcttcg ggcaagtctt tttctgtttg
      841 acacctccaa ggagtggccg aatgaccaga tttcagtgag cgcctgtctt gttgctccag
      901 cgaacgcagg atggattgct ggacataatg aaggggtgcg cattccccaa caaggaacaa
      961 gttgctggaa ctggatggca tacaacacgc gcccttgggc agga
//
LOCUS       DAOQUC010001692         1003 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_229307_length_1003_cov_2.995781, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001692 DAOQUC010000000
VERSION     DAOQUC010001692.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1003
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_229307_length_1003_cov_2.995781"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            99..>1003
                     /locus_tag="PKH07_20940"
     CDS             99..>1003
                     /locus_tag="PKH07_20940"
                     /inference="COORDINATES: protein motif:HMM:NF024909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HNT37474.1"
                     /translation="MIYAKPDILWLLLLMPLIWIVHFRGRPLPSLSVSSVTFWRGLRA
                     ETTASKRKRTIHWLAVLVETLFLSTFILAWSEPTLRQSERLPEMSDVIIVVDNSLSMS
                     SMESQKEIRTAYARLLDTLAPDSRVNVVSWSDRVIFVANGTPSNAHVRRVLEQIAPVR
                     ASNRMDRLTDYVHAFRTAHPESHCILLSDGRFQIDGSTDVPEFLYCPIQSNGDNIALT
                     EVSAKADTLENVLLHVHCKSFSEQTVTGTLRVLSKSRTVASVSVTIPAGSESITAIEP
                     SVPLSEISHISLDVTDTLGLDNVLS"
BASE COUNT          233 a          279 c          236 g          255 t
ORIGIN      
        1 tgtgccttat ttccttgcat tcacagagtc ctctgtcacg acaacactga tcgagtttct
       61 cagacagaag gggatgctcc gctagtgcat ggagaacaat gatctacgcc aaacctgaca
      121 tactctggct cctccttctg atgccgctga tctggattgt gcatttcaga ggcagaccct
      181 tgccgtcttt gtccgtctcc tctgttacat tctggagagg actgcgagca gagacaacag
      241 catcgaaacg aaagcgaacg atacactggc ttgccgtact cgtagagaca ctctttctgt
      301 ccacttttat tctggcgtgg agtgagccga ctctgagaca gtcagaacgc ttgcctgaga
      361 tgtcagatgt tatcattgtg gtggacaatt ccctgagcat gtcgtcaatg gagtctcaga
      421 aggagattcg cacagcctac gcccgtcttc tggacaccct ggctcctgac tcccgagtca
      481 atgttgtctc ctggtcggac agagtgatct tcgtcgccaa tggaactcca agcaacgcac
      541 acgttagaag ggttctcgaa cagatcgctc ccgtgcgtgc gtcgaacagg atggatcgcc
      601 tcaccgacta tgttcatgcc ttccgaacgg cgcaccctga gtctcattgt attctgctct
      661 cggatggaag attccaaata gatggctcca cagatgtccc agagttcctg tattgcccga
      721 tccaatccaa tggcgacaac atcgctttga ctgaggtgtc cgctaaggca gacactttgg
      781 aaaacgtcct tctgcatgtt cactgcaaga gtttctcgga acagaccgtc acaggaacac
      841 ttcgcgtcct ttccaaatcc agaacggtcg caagcgtctc agtaacaatc cccgccggat
      901 cagaatctat caccgctatc gagccgagtg ttccattgtc ggaaatctca catatcagtc
      961 tggatgtgac ggacacactt ggtcttgaca atgtactctc ttt
//
LOCUS       DAOQUC010001693         1002 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_229688_length_1002_cov_3.616684, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001693 DAOQUC010000000
VERSION     DAOQUC010001693.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1002
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_229688_length_1002_cov_3.616684"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="PKH07_20945"
     CDS             complement(<1..214)
                     /locus_tag="PKH07_20945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37475.1"
                     /translation="MNTKEPTLVLIDLIRVVKVTLLYFYLANNIRTREEIRLVVWSLI
                     AGASIQGCVTLVQAYLGRPIGLHLFGE"
     assembly_gap    225..234
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<235..650)
                     /locus_tag="PKH07_20950"
     CDS             complement(<235..650)
                     /locus_tag="PKH07_20950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37476.1"
                     /translation="MTHFGKGKQLVARLAVVTFLGVSIGLALSIVLGLQLRYLLVGLF
                     VVLVPLTVQMTGRWKQFFLIALMLGIPLQIKKTIFPYSITHVGGPGGIDVLFADGALI
                     ALYLHWALMTITRRQERAIRITGIDLLVALLGAICLL"
     gene            complement(718..>1002)
                     /locus_tag="PKH07_20955"
     CDS             complement(718..>1002)
                     /locus_tag="PKH07_20955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNT37477.1"
                     /translation="YCGKLEETRISEALDLERISNVTVIEPAIVPFEPIRIIPFLPTR
                     VLHILAGVIVGLLSALGYAFLTEQMNHTFSSREDVDRMLNIRCLVSIPKE"
BASE COUNT          253 a          263 c          259 g          217 t
ORIGIN      
        1 cttctccaaa aaggtgcagc ccgatcggac gtcccagata cgcctgcacg agtgtgacgc
       61 atccctggat gcttgcgccg gcgatgagtg accacacgac aaggcgaatc tcctctcgtg
      121 tgcgaatatt gttggcgagg tagaaataga gcagagtgac cttcacgaca cggataaggt
      181 caataagaac gagggtcggc tctttcgtgt tcactaatga aagcnnnnnn nnnnagcaga
      241 caaattgcgc ccagaagtgc gaccagcaaa tcaatgccgg tgatacggat tgcccgctcc
      301 tgccttctcg tgatcgtcat cagcgcccaa tgcagataaa gcgctatcag tgcaccgtcg
      361 gcgaaaagaa cgtcaattcc acccgggcct ccgacatgtg ttattgaata gggaaagatc
      421 gtcttcttga tctgcagcgg gatgccgagc atcaaagcga taaggaaaaa ctgcttccat
      481 cgtccagtca tctgtacggt caaaggaacg agcaccacga acagcccaac caggagatat
      541 ctcagttgca gtccaagcac aatcgataga gcaagcccga tcgacacgcc taggaacgtg
      601 acgactgcca gacgagcaac aagctgtttt cccttcccga aatgggtcaa gattgtgttt
      661 ctcgactttt catggcttct gcaggttggt tggtcagcga actgcctccg tcactgctca
      721 ctccttcggt atcgagacca ggcatcggat gttgagcatt ctgtccacgt cttccctcga
      781 gctgaaagta tgattcatct gttctgtcag aaacgcgtat ccaagtgcgg agagcagtcc
      841 gacgatcacg cctgcaagga tatggagcac acgagttggg aggaatggaa tgatgcgaat
      901 gggctcaaat gggacaattg ccggttcgat caccgttaca ttggagatcc gctcgagatc
      961 cagtgcctcc gatattcgag tctcttccag tttgccgcag ta
//
LOCUS       DAOQUC010001694         1002 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_229696_length_1002_cov_3.463569, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001694 DAOQUC010000000
VERSION     DAOQUC010001694.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1002
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_229696_length_1002_cov_3.463569"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..970
                     /locus_tag="PKH07_20960"
     CDS             <1..970
                     /locus_tag="PKH07_20960"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNT37478.1"
                     /translation="GVDPSDVPMGLVPVCLSPELPQAQMDYRSGSAREIRFIQGGIFW
                     PWQDSSIFLNRILNVLERRSSGRVQLFGGHHPHHQIKGEKYLDPAERLKPGSRWEVLP
                     MRSAEDLFADYARGGVAVDLMATNVERELSSAIRNVSYLWCGLPLICFDYSYLASDIS
                     SYQAGWVLSRDGQGLEELVESLLKQPDEIVRRGECAQQLVREKYTWTTAANSLLSFCD
                     AARVRSKRPTFMQASSTMLEQQERLIHEYTSRLQITEKDIARRDEEISKKDWIIAKRD
                     EEIMKLHQKLGECEARASGAERDLHTIRSKFVFRLYKRIVKLLGGE"
BASE COUNT          239 a          230 c          285 g          248 t
ORIGIN      
        1 cggtgttgac ccgagtgatg ttccgatggg tctggtgcct gtttgccttt cccctgagct
       61 tccccaagcg cagatggact accgttccgg cagtgccaga gaaatccgtt tcatccaagg
      121 cgggatattc tggccctggc aagattcctc gatttttcta aaccgtatcc tgaatgttct
      181 ggagcggcgg agttccggtc gagtgcaact ttttggaggt catcatccgc atcatcagat
      241 caaaggcgag aagtatcttg atccagccga aaggctgaag ccgggatcgc gctgggaagt
      301 tctgcccatg cgttcggcag aggatttgtt tgccgactac gcacgcggtg gagttgctgt
      361 ggacttgatg gcgaccaatg tcgaacggga actgtcttct gcgattcgaa atgtgagcta
      421 tctctggtgt gggctgccgc tcatctgttt cgactacagt tatctggcat cggacatctc
      481 ttcttatcaa gccggatggg ttctgagtcg ggacggtcag ggtttggagg agttggtcga
      541 gtctctgctc aagcaacctg atgaaatagt ccgacgtggt gaatgcgccc aacaacttgt
      601 tcgtgagaaa tacacttgga cgaccgctgc caacagtctt ctttcatttt gtgatgctgc
      661 cagagtgcga tccaaaagac cgactttcat gcaggcaagt tcgacaatgc tggaacaaca
      721 agagcggctg attcacgaat acacgtctcg tcttcagatc acagagaaag acatagccag
      781 gcgggatgag gagatcagca agaaggactg gatcatcgcc aagagagatg aagaaatcat
      841 gaagttgcat cagaaacttg gcgagtgtga ggcacgggcg agtggagctg aaagagacct
      901 acacacgatc cgaagcaagt ttgttttcag actgtacaaa cgtattgtga aacttcttgg
      961 tggggagtag ttttgcaccg ccattgacgt actttgcaga ta
//
LOCUS       DAOQUC010001695         1001 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1124
            NODE_230142_length_1001_cov_2.802326, whole genome shotgun
            sequence.
ACCESSION   DAOQUC010001695 DAOQUC010000000
VERSION     DAOQUC010001695.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852467
            Sequence Read Archive: SRR4236649
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:28:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,194
            CDSs (total)                      :: 4,157
            Genes (coding)                    :: 4,116
            CDSs (with protein)               :: 4,116
            Genes (RNA)                       :: 37
            tRNAs                             :: 35
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 5 of 41
            Pseudo Genes (incomplete)         :: 34 of 41
            Pseudo Genes (internal stop)      :: 2 of 41
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_230142_length_1001_cov_2.802326"
                     /isolate="AMR_MDS_1124"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..739)
                     /gene="fdhD"
                     /locus_tag="PKH07_20965"
     CDS             complement(<1..739)
                     /gene="fdhD"
                     /locus_tag="PKH07_20965"
                     /inference="COORDINATES: protein motif:HMM:TIGR00129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HNT37479.1"
                     /translation="MLNETAKDSCTWNVVRLTDCGQNRLGEDSCLAIEDVLTLEIQDV
                     GSYTLMCTPSEVLALAVGFAFTEGLIETIDDIGTLSQCPDEPLTIRMQRVGSRSVSET
                     PRNLIVSTACGMCGGRKAVEEILSGMNKVSETLSLSTALFPVIMEEMRKRQTLYLKTG
                     GAHAAAIFDKDGQIVSVAEDVGRHNALDKAIGKSLLARIPLCGKGVALSGRVSLEMVS
                     KAARAGLELIAAVSAPSSLGVESADRCN"
     gene            complement(759..>1001)
                     /locus_tag="PKH07_20970"
     CDS             complement(759..>1001)
                     /locus_tag="PKH07_20970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor synthesis protein"
                     /protein_id="HNT37480.1"
                     /translation="APDVVTDFCEKLIPGIMDHIRLKCGADSPNSLISRSLAGVSGKM
                     LVFALPGSMKAVREYLDEILTLLEHLLLMVRGIDAH"
BASE COUNT          257 a          293 c          229 g          222 t
ORIGIN      
        1 tgttacagcg atcagcactc tcgacaccca gggatgacgg cgccgagacc gcagcaatga
       61 gttcaagtcc agcacgagcc gccttggaga ccatctcaag actcacgcgt cccgaaagtg
      121 caacgccctt accacaaaga gggattcgtg caaggagaga tttgccaatt gccttgtcca
      181 gagcgttgtg cctccccaca tcctcagcga cggagacaat ctgaccatcc ttatcgaata
      241 tcgctgccgc gtgtgcgccc cccgttttga gataaagggt ttgccttttg cgcatctcct
      301 ccataatcac tggaaacagg gctgtactga gagagagcgt ctccgagact ttgttcatgc
      361 cggagaggat ctcttcaacc gcctttcttc caccgcacat gccacaagca gttgagacga
      421 tcaggtttcg cggtgtttcc gaaacgcttc gagaacccac cctttgcatc cgaattgtca
      481 aaggctcatc gggacattgt gaaagcgtac caatgtcgtc tatcgtttcg atcaagccct
      541 cagtgaaagc aaagccgaca gcgagcgcga gaacctcgct tggcgtacac atcagtgtat
      601 atgaacccac gtcctgaatc tccagagtca aaacatcttc aatggcaaga caagagtcct
      661 caccgagtct gttttggcca caatcagtca gtcgcacgac gttccatgtg catgaatcct
      721 tggctgtctc gttcaacaca aattgcgtcc tccccaggtc aatgagcatc aattccacgc
      781 accatcaaga gaaggtgttc gagaagcgtc aatatctcat ccaggtattc ccgaacagct
      841 ttcatactcc ctgggagagc aaagacaagc atctttccag aaactccggc cagactccga
      901 ctgatgagcg aattcggact atctgcacca cacttgagtc gaatatgatc catgatccct
      961 gggatcaact tctcgcagaa atcggtgacc acatctggag c
//
